LOCUS       DATGEM010000001         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1484149, whole genome shotgun sequence.
ACCESSION   DATGEM010000001 DATGEM010000000
VERSION     DATGEM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484149"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VKQ27_00005"
     CDS             complement(<1..594)
                     /locus_tag="VKQ27_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87340.1"
                     /translation="MSSTIPALLPRGPGHQFVVYGDACSGVPGAPHERSFAEVNAVIR
                     RLWPQPEFIIFTGDEIRGLTADPDDLRCQWRHWLDHEMAWLDRRATPLWHVTSNHTAY
                     DPPSEAIFREMLDLPRNGPPGQEGLSYWVRRDDLLLVFVHTLWSGLGGEGHVEIAWLD
                     AVLHQHADARTKLVVGHHPAFPVNGFAGAYERDIGPEH"
     gene            675..2171
                     /locus_tag="VKQ27_00010"
     CDS             675..2171
                     /locus_tag="VKQ27_00010"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLY87341.1"
                     /translation="MREIGHFIAGKPVAGTSGKFGDVFNPASGELSARVALADTSEVN
                     KAVAAAAAAWPAWAATPPLRRARVMFKLKELLERDRKEISAIITAEHGKVLSDADGEV
                     QRGLEVVEFACGIPHLLKGEFTEAVGTGIDAWSIRQPLGVVAGITPFNFPLMVPLWMI
                     PVALACGNCFVLKPSEKDPSAALKLAALLAEAGIPDGVFSVVQGAREAVEAIMAHPDI
                     AAISFVGSTAIAETIYRGGAAHGKRVQALGGAKNHLVVMPDADIDDAVDALMGSAYGA
                     AGERCMAVSVAVAVGTAGDRLMQKLEPRVRALKIGPGTDPDAEMGPLVTREHREKVQG
                     YIDLGVKEGAKLVVDGRGLALQGYENGFFLGGSLFDNVGTDMRIYKDEIFGPVLGVVR
                     TPDMAGAVRMVNAHEYGNGVAIFTSDGGAARDFAAGINIGMVGVNVPIPVPMAFHSFG
                     GWKHSLFGDHHIYGPEGVRFYTRYKAVTQRWPTGARRGAEYVMPTLGR"
     gene            2557..>3871
                     /locus_tag="VKQ27_00015"
     CDS             2557..>3871
                     /locus_tag="VKQ27_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87342.1"
                     /translation="MTRTLWIMAILLTFGGFFDGYAIGLIGALGPGLFKAGIFTPTTV
                     SFFGFSGFASFVAALFAGFFLASLFVSYVADHLGRRAIFAYSLLWFGIANFIMGMQTT
                     ADGVNLWRFISALGVGLELVTVDAYLSELVPKKTRGAAFAFLQAMSAIAFLVSYFLSW
                     QLIPLAPFGYDGWRWVTWVGSIAAIVIWWIRLGLPESPRWLAQQGRMAEAERVMEHIE
                     GRVAADYGRPLPQPETPHYQDPRQGSIWEIFGPRYLKRTIMLMLFNFFQTVGFYGFVA
                     WTPTLLIAKGIHITQSLEYSTIINVAAVAFPLLIMLFADKIERKWHCCLSCLCIALFG
                     IWFASAEGSWALIALGACLAMAAQWLSYSLHNYQAELYPTRIRARGVGFVYSWSRLSG
                     ILTPFFIAFFLRDFGPTGVFLFVAGCMAVVIVSVAALGPATRRLEV"
BASE COUNT          626 a         1094 c         1385 g          766 t
ORIGIN      
        1 gtgttccggc ccgatgtccc gctcataggc gcccgcgaag ccattcacgg gaaacgctgg
       61 gtggtgaccg acaacgagtt tggtgcgcgc gtcggcatgc tggtgcagca cggcatcgag
      121 ccaggcgatc tcgacgtgac cctcgccgcc gaggccgctc catagggtat ggacgaagac
      181 gagcagcaga tcgtcgcgcc ggacccagta ggacaatccc tcctggccag gcggcccatt
      241 gcgcggcagg tcgagcattt cgcggaagat cgcctcgctg ggcggatcgt aggcggtgtg
      301 attgctggtc acatgccaca gaggtgtggc gcgccggtcg agccaggcca tttcgtggtc
      361 gagccagtgg cgccattggc agcggaggtc gtcgggatcc gccgtcaggc cgcggatctc
      421 atcacctgtg aagatgatga attcgggttg tggccacagg cggcgaatga ccgcgttgac
      481 ttccgcgaag ctgcgttcgt gtggtgcgcc tggcacgcct gagcacgcgt caccatagac
      541 cacgaactgg tggcctggac cgcgtggaag cagcgccggg atcgtggagg acatggccgc
      601 tgaattagca ggagtctggc gggctggcac gatccgtcct aagtccctga gtggatcgac
      661 gggagggatg caacatgcgc gaaatcggcc atttcatcgc gggcaagccg gtggcaggca
      721 ccagcggcaa gtttggcgac gtgttcaatc cggcctcggg cgagctgtcg gcgcgcgtgg
      781 cgctggccga taccagcgaa gtgaacaagg cggtggccgc tgctgccgcg gcctggccgg
      841 cctgggctgc gacaccgcca ctgcggcggg cgcgcgtcat gttcaagctg aaggaattgc
      901 tggagcggga tcggaaggag atttcggcga tcatcaccgc cgagcacggc aaggtgctgt
      961 cggacgctga cggcgaggtg cagcgcggat tggaagtggt ggagttcgcc tgcggcatcc
     1021 cgcacctcct gaaaggcgag ttcaccgagg ccgtcggcac aggcatcgat gcgtggtcga
     1081 tccgtcagcc tctgggcgtg gtggcgggga tcacgccgtt caacttcccg ctgatggtgc
     1141 cgctgtggat gatcccggtg gcgctcgcgt gcggcaactg cttcgtgctg aagccgtcgg
     1201 agaaggatcc ctcagcggcc ctgaagctgg cggcgctgct ggccgaggcg ggaattccgg
     1261 acggcgtatt cagcgtggtg caaggcgcgc gcgaggcggt ggaggcgatc atggcgcatc
     1321 ccgacatcgc ggcgatcagc ttcgtcgggt ccacggcgat tgccgagacg atctatcgag
     1381 gcggtgcggc acatggcaag cgcgtgcagg cgctgggtgg ggcgaagaac cacctggtgg
     1441 tgatgccgga tgccgatatc gacgatgcgg tggatgcgct gatggggtcc gcgtatggcg
     1501 cggcgggtga gcgctgcatg gcggtgtcgg tcgcggtggc cgtggggacc gcgggcgatc
     1561 ggttgatgca gaagctggag ccgcgggtgc gggcgctgaa gatcgggccg gggacggatc
     1621 ccgatgctga gatgggcccg ctggtgacgc gcgagcatcg ggagaaggtg caggggtata
     1681 tcgacctcgg cgtgaaggag ggtgcgaagc tggtagtgga tggccgtggg ctggcgctgc
     1741 aaggttatga gaacggcttt ttccttggcg ggtcgctgtt cgacaatgtc gggacagaca
     1801 tgcgtatcta caaagacgag attttcgggc ccgtgctcgg cgttgtgcgc acgcccgaca
     1861 tggccggcgc ggtgcggatg gtgaatgcac atgagtatgg gaatggcgtg gcgatcttca
     1921 cttcggacgg tggtgctgca cgggatttcg cggcgggcat caatatcggc atggtggggg
     1981 tgaatgttcc gatccccgtg ccgatggcgt tccattcgtt cgggggatgg aagcattcgc
     2041 tgttcgggga tcaccatatt tatgggccgg agggcgtgcg gttctacaca cgatacaagg
     2101 cggtcacgca gcggtggcca acgggggcac ggcgcggcgc ggagtatgtg atgccgacgc
     2161 tggggaggta ggcagggtcc ggttggacca gcgcatcctt ggcaaatgcg ctcagggtgc
     2221 gctcgccggt cgagattgct tcgcccgctc gcaatgacag gggaggcgtt cgcgtcgcgt
     2281 gtgaatgcgg tgcgctttac acgacgcggg atattggctc agtctcaata gtggctcgcg
     2341 aatagagctg gactacggct cacggcacgc aacggcgtcc ggaacccgtg gcatccgaat
     2401 aagaccaggg actggaccaa ggaaacgaaa atgacacccg catcagatgc tgcggcagcc
     2461 gcaaacggag agcctctagc cttcggcgaa gacggtctct cctcccgcac tgctacagca
     2521 gccgaacgac tgacggcccg gctggaccgc ctgccgatga cccgcacctt gtggatcatg
     2581 gcaattctgc tgactttcgg agggttcttc gatggctatg cgatcggcct gattggcgca
     2641 ctcggcccgg ggctgttcaa ggccggtatc ttcactccga cgactgtgag cttcttcggc
     2701 ttcagcggct tcgcgagttt cgttgccgcg ctgtttgccg gcttcttcct cgcgtcgctg
     2761 tttgtttcct atgtcgccga ccatctgggc cggcgcgcga tattcgccta ctccctgctg
     2821 tggtttggca tcgccaattt catcatgggg atgcagacga cagcggacgg cgtcaacttg
     2881 tggcgcttca tcagcgcgct tggtgtcggc ctcgaactgg ttactgtcga cgcctatctc
     2941 tccgagctgg tgccaaagaa gacacgtggc gcggcctttg cgtttcttca ggctatgagc
     3001 gccatcgcgt tcctggtgtc ctatttcctg tcctggcaac tgatcccgct ggcaccgttc
     3061 ggctatgacg gctggcgttg ggtgacgtgg gttggttcga ttgcggccat cgtcatctgg
     3121 tggatcaggc tgggcctacc ggaaagcccg cgctggctgg cgcagcaggg gcggatggcg
     3181 gaagccgagc gcgtcatgga acacatcgag gggcgggtcg ctgccgacta tgggcggccg
     3241 ctgccgcagc cggagacgcc gcactatcag gacccgcggc agggcagcat ctgggagatc
     3301 ttcggcccgc gctacctgaa gcgcacgatc atgctgatgc tgttcaactt cttccagacc
     3361 gtcgggttct acggttttgt ggcgtggacg cccactctgc tgatcgccaa gggcatccat
     3421 atcactcaga gcctggagta cagcaccatc atcaatgtcg ccgcggtcgc tttcccgctg
     3481 ctgatcatgc tgtttgctga caagatcgag cgcaaatggc actgctgcct gtcgtgtctt
     3541 tgcatcgcgc tgttcggcat ctggttcgcc agcgcggaag gctcgtgggc actgatcgcg
     3601 ctgggtgcgt gtctggcgat ggcagcgcaa tggctctcct actcgctgca taattaccag
     3661 gcggaactgt atccgacgcg gatccgtgcg cgtggggtgg ggttcgtcta ctcctggagc
     3721 aggctcagcg gcatcctgac gccgttcttc atcgcattct tcctgcgtga ctttggcccg
     3781 acgggcgtct ttctgtttgt cgcgggatgc atggcggtgg tcatcgtgtc ggttgcggcg
     3841 ctagggccgg cgacgcggcg gttggaagtg g
//
LOCUS       DATGEM010000002        10506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_682252, whole genome shotgun sequence.
ACCESSION   DATGEM010000002 DATGEM010000000
VERSION     DATGEM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682252"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1599)
                     /locus_tag="VKQ27_00020"
     CDS             complement(37..1599)
                     /locus_tag="VKQ27_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLY87343.1"
                     /translation="MPKRGISAVLLGSTAAVLLFAGTARADDMADLKAQIKALSDRLG
                     QIEAERAAAAKVPSVQTTTGAPPQTSVPATLRKDESGRTPGTPSSTAVAVDTSVPATL
                     QKDQGGNAIGILNNPVMLYDDKNTSVRLYGLIEATLGIANNQNAKGSLAVGYQTAWFS
                     GNRLGFDAFHALSFGDDIGLPGLKVVSKLETEFESPTGNADTPGVIFNRDAWVGLESD
                     DLGKITLGRQNTLTRDFTQNWGDAYGTPEVTMKEGGYTNVNNFKQLIFYSASSTSTRN
                     NSGIEWKKHFGDHWLVGVGYAFGSGGVGGSGNGGPGQFNAGGGGTPGQFTLGSNQAVS
                     VAYNDLAIGPGKANFNVSYNRANNHDLINQAVLAGGNYSIGPFRVNAGYIHYTGEQGV
                     NNSAGTRTDNAWTVSGSYWPAPKIETDIGYVRMDGTHAGNCGGNTLLPFLQDASCVTR
                     ATVANGGKGTVFGSLIFHADKQTDFYLASDYMKVDGGWSVGDAQGNVDGIGHGRAHSD
                     ELEVATGLRFRF"
     gene            1872..2210
                     /locus_tag="VKQ27_00025"
     CDS             1872..2210
                     /locus_tag="VKQ27_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLY87344.1"
                     /translation="MHDHLSQSIAREVLRLALQIAPTLGQVPPSDADATTRPKDLSLQ
                     LYRQRLLALDRGLTGREIDVCARSLSGMTAEGIGLELEIKKSSVVTYRKRAYERLGIS
                     SQHELFRMLA"
     gene            2312..2983
                     /locus_tag="VKQ27_00030"
     CDS             2312..2983
                     /locus_tag="VKQ27_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87345.1"
                     /translation="MRILIVEDNRKLSDWIAMLLRRSKYVIDCVYDGEDADYALRTQD
                     YSLVILDLTLPLLSGTEVLSRLRARGSTTPVLVLTANDSVPSRVSALDGGADDYLAKP
                     FNIEELEARIRARLRRRHEPMSPTAHYGILSLDRNSGQFFLGGTDLVLTPREHVVLES
                     LILKEGATVSKTSLMESVFGFGDGGNVNSIEIYVHRVRKKLEGSGVGIVTFRGLGYAL
                     RQDPC"
     gene            2977..4356
                     /locus_tag="VKQ27_00035"
     CDS             2977..4356
                     /locus_tag="VKQ27_00035"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87346.1"
                     /translation="MLSSSLHLQLLRWILAPLFVLVLFNAWQSYSNARLVAGIVTDRT
                     LAGSAHAIAEQIKVVDGLAEAIVPPSALEMFASGFQDRVVYRVTNADGTLLAGYSDVP
                     GAAQATENLEPRYFDAMFRGQPIRAVALSQPLIGGGDRRFALVIVGQTLRGHRRMIAD
                     LWVAETERQLALVVIAAALAWFGLNRGLAPLARLSEEVAARTPDTLDPIATDRVQKEL
                     RPLVAALNHSIERVRNQIVVQRRFIADAAHQLRTPLTLLKTQIFYGLSTVDLDEKNKS
                     LSAVDESVGQMVHLTNQLLMLAKIEPGALSIVREPVDLAASARQVLEAVADRALDRNI
                     ELSFDAAPVRVLANRAMLHELIMNIVDNALRYSPTGSAINAAVSAGDGVGILRVADQG
                     PGVPVHERERVFDRFYRILGTDTEGSGLGLSIVREIAWRYGGTVTLADSPLGPGLVVE
                     VTLPAASEA"
     gene            complement(4366..5364)
                     /locus_tag="VKQ27_00040"
     CDS             complement(4366..5364)
                     /locus_tag="VKQ27_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87347.1"
                     /translation="MKSSRFRMVLAMLLSTVTTAAHGGDRVTLMVGGIEKLIYLPAAL
                     GEQLGAFQEQDLDVEIRSEPVGNEAEDELLAGAVDGVVGFYDHAIDLQARGKFIESIV
                     LFGQTPGEVELIPARLATEIRSPADFKGRTLGVTGLGSSTDFLTQYLAVKHGVPLRDI
                     SLLPIGAGDTFIAAMLDGRIDAGMTTEPTASRLLKTGGAEILVDMRTPDATREALGGT
                     YPAACLYVQSSWVRGHRDLAQRLANAFVKTLRFIDTHSAAEIADHMPSEYFQGDKSGY
                     IQLLAQSKSRFTPDGKMPIDGPPTVLAVLSGFSQNLRGKTIDLSKTYTDEFVNAAK"
     gene            complement(6798..7508)
                     /locus_tag="VKQ27_00045"
     CDS             complement(6798..7508)
                     /locus_tag="VKQ27_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87348.1"
                     /translation="MDFRDRHVVITGGTGALGRAVVGALLAAGAHCHVPYRQEQEKEG
                     FPTGALSLIPVPSLADEAAVVKLYGSLPSLWASIHVAGGFALAPLTDTSGELLRRLID
                     DNLVNCFLCCREAVVAMRRGKAGGRIVNVAARQALEPRTGATMSASTAAKAGVAALSE
                     ALGEELAGEGILVNAVAPSIIDTPTNRRNFPKANFDAWPKVEEVAATILFLASPENKV
                     SRGGVLPVYGASFQSAAS"
     gene            complement(7874..8629)
                     /locus_tag="VKQ27_00050"
     CDS             complement(7874..8629)
                     /locus_tag="VKQ27_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87349.1"
                     /translation="MKIVVIGGTGLIGAKTVAILRQGGHEAVAASPNTGVNTITGEGL
                     KEALAGAEVVIDLANSPSFEDKAVLEFFETSERNLLPAEAAAGVRHHVALSIVGADRS
                     PDIGYFRAKVAQERLIEAAGIPYTIIHATQFMEFLGGIADSGADGNMVRISPGLFQPI
                     AADDVAAIVAELALAAPRNGFVEIAGPERAPFSEIVARYLKAVGDPRQVVSDPEARYF
                     GGRVEEHSLVPLGEARLGRIGLDEWLRRSKAGA"
     gene            8763..9119
                     /locus_tag="VKQ27_00055"
     CDS             8763..9119
                     /locus_tag="VKQ27_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87350.1"
                     /translation="MEFLGGIADSAMDGNKVRLSPGLFQPIAADDVAAIVAEVALAAP
                     RNGIVEIAGPERAPLDEIIARYLKAVGDPREVVCDPEARYWGGRVEERSLVPLGEARL
                     GRIGLDEWLRRSQARA"
     gene            9505..>10506
                     /locus_tag="VKQ27_00060"
     CDS             9505..>10506
                     /locus_tag="VKQ27_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLY87351.1"
                     /translation="MVESDTVARRELKSDELSKTRRPQKRHEFDCIILLLQGGGALGA
                     YQAGVYEALAEADLHPNWVAGISIGSINAAIIAGNPPDKRVEKLREFWEGITNQPFDL
                     IGGELGSVMARGEVARTSLNQLSAAVALLTGASGFFRPRILNPWFHPTGTIEATSYYD
                     TIQLKATLESLVDFDRVNSNNANPRLSLGAVNVRSGNLIYFDSSRQAIQPEHVMASGA
                     LPPGFPAIEIEGEHYWDGGLVSNTPLQWVAMTQRPPDALAFQVDLWSARGEFPRNLAE
                     VATRQKEIQYSSRTRAFTDYFKRVIKLRNTVAELLEELPEEMRQSEKVKFLTANVERH
                     "
BASE COUNT         1887 a         3337 c         3340 g         1942 t
ORIGIN      
        1 cgggtttcga tatgaccggg cgtgaagccc ggccgctcag aatctgaagc gcaggccggt
       61 cgcgacctcg agttcgtcgc tatgcgcccg gccgtgcccg ataccgtcga cgttgccctg
      121 ggcgtccccg accgaccagc cgccgtcgac tttcatgtag tccgaggcca ggtagaaatc
      181 ggtctgcttg tcggcgtgga agatgagcga gccgaacacg gttcccttgc cgccgttggc
      241 gacggtcgcg cgggtcacgc acgaggcgtc ctgcagaaac ggcagtagcg tgttgccacc
      301 gcaatttccg gcatgcgtgc cgtccatgcg gacgtagccg atgtccgtct cgatcttcgg
      361 cgctggccaa tagctgcccg agactgtcca ggcattgtcg gtgcgcgttc cagcactgtt
      421 gttgacgccc tgttcgcccg tgtagtggat gtagccggca ttcacccgga aaggcccgat
      481 cgaataattg ccgccggcca atacggcttg gttgatgaga tcgtgattgt tggcccggtt
      541 gtaactcacg ttgaagtttg ccttgccagg gccaatcgcg agatcgttat aggcgaccga
      601 cacggcttgg ttgctgccga gcgtgaactg tccgggcgtg ccaccgccgc cggcgttgaa
      661 ctggcctgga ccaccattgc cgctgccgcc gacgccgccg gagccgaagg cgtagccgac
      721 gccaaccagc caatggtcgc cgaagtgctt cttccactcg atcccgctgt tgttgcgcgt
      781 gctggtcgaa ctcgccgagt agaagatcag ctgcttgaaa ttattgacgt tggtgtagcc
      841 accttctttc atggtcacct cgggcgtacc gtaggcgtca ccccagttct gggtaaaatc
      901 gcgggtcagc gtgttctggc ggcccaaagt gatcttgccg agatcatcgg actcgagacc
      961 gacccaggca tcccggttga agatcacgcc gggggtgtcg gcattgccgg tcggggattc
     1021 gaactcggtc tcgagcttgg aaaccacctt gaggcctggc aggccgatat cgtcgccgaa
     1081 gctcagggca tggaaggcat cgaagcccag gcggttgccg ctgaaccagg ccgtttgata
     1141 gccgaccgcc aaactgcctt tggcgttctg gttgttcgcg atgccgaggg tcgcctcgat
     1201 caggccgtag agacgcacgc tcgtattttt gtcgtcgtac agcatgaccg ggttgttcag
     1261 gatcccgatc gcattgccgc cctggtcctt ctgcaaggtc gcggggaccg acgtgtcgac
     1321 agccaccgcc gtgctgctcg gcgtgccggg cgttctgccg ctctcgtctt tccgcagggt
     1381 tgccggcacc gaagtctgcg ggggcgcccc cgtcgtcgtc tggaccgacg gcaccttggc
     1441 cgccgcagcg cgctccgctt cgatctggcc gaggcggtcg ctcaaggcct tgatctgcgc
     1501 cttaagatcg gccatgtcgt ccgcgcgagc agtgccggca aacagcagaa ccgccgcagt
     1561 agatccaagt aaaaccgccg atatgccacg cttgggcatg tatcttcccc cctgaatttt
     1621 tttaaaaacg ggcccgccct gaaaaacgac gttttccagc caacgtgcga tgctacattt
     1681 ccagtgaatg gtcgcatttc accgcgacct aatctgacca tttcaagggg gacaggctgt
     1741 ccccacaggt ggggaaacag ggctatgcct tgaaagcaaa ctgaaagatt ggaagtgcta
     1801 gagttcggca agcgtttcga ccggaaggcc ggcggctcgc cagagaaata taaaaattta
     1861 gggagcggag aatgcacgat cacttgtcac agtcgatcgc ccgggaagtg cttcgactgg
     1921 cgcttcagat cgcgccgacc ttgggacaag tgccaccgag cgacgcggac gccacgacgc
     1981 gtcccaaaga cctgtcgctc caactttacc ggcaacgtct gctggcgctg gatcgcggcc
     2041 tcaccggccg cgagatcgac gtttgtgccc gctcgctttc cgggatgacg gcggaaggca
     2101 tcgggctcga actcgagatc aagaagagca gcgtcgtcac ctaccggaag cgggcgtacg
     2161 agcggctcgg catttcctcg cagcacgaac tgttccggat gctcgcctga gtcatgctcg
     2221 gtcgtgagcg tcgcgttctg ccgaacggca gtgtaccgtc gtcgatggga gacggcggcc
     2281 gtacccccgg gcacggtccg agagggacga gatgcggatt ctgattgtcg aggataatcg
     2341 gaagctgtcc gactggatcg ccatgctgct gcgccgcagc aaatacgtga tcgattgcgt
     2401 ctatgacggc gaagatgccg actatgccct gagaacccag gactattcgc tggtcatcct
     2461 cgacttgacc ctgcctctgc tgagcggtac agaggtactg agccgcctca gggcgcgcgg
     2521 cagcacaacg ccggtgctgg tcctcaccgc caacgacagc gtgccgagcc gtgtgtcggc
     2581 gctcgacggc ggggccgacg attatctcgc gaaaccgttc aacatcgagg aactggaagc
     2641 acgcatccgc gcacgcctgc gccggcggca cgaacccatg agcccgaccg cgcactacgg
     2701 catcctctcg cttgatcgga acagcgggca atttttcctg ggcggcaccg atctcgtgct
     2761 caccccgcgc gagcatgtcg tgctggagag cttgatcctc aaggaaggtg caacggtgag
     2821 caagaccagc ttgatggaga gcgtcttcgg cttcggcgat ggcggcaacg tcaattcgat
     2881 cgagatctac gtccaccggg tacgcaagaa gctcgaaggc agcggtgtcg gcatcgtcac
     2941 gttccggggc ttgggttacg cgcttcgcca ggatccatgc tgagcagcag cctgcacctg
     3001 cagctgctgc gctggatcct ggcgccgctc ttcgtcttgg tgttgttcaa cgcctggcag
     3061 agttattcta atgcccggct cgtcgccggc attgtcacgg accgcacgct ggccggctcc
     3121 gcgcatgcca tcgccgagca gatcaaggtc gtcgacgggc tggccgaggc catcgtgccg
     3181 ccctcagcgc ttgagatgtt cgcctcgggc ttccaggatc gggtggtcta tcgcgtcacc
     3241 aacgccgacg gcacgttgct tgcgggctat tccgacgtcc ccggggcggc gcaggccaca
     3301 gaaaatctcg agccccgcta tttcgacgcg atgtttcgag ggcagccgat ccgggccgtg
     3361 gcgctcagcc agccgctgat cggcggcggc gaccggcgat tcgcgctcgt catcgtcgga
     3421 cagacgctgc gcggccatcg gcgcatgatc gccgacttgt gggtggcgga gacagagcgt
     3481 cagctggcgc tcgtcgtcat cgcggcggcg ctcgcctggt tcggcctcaa tcgcggcttg
     3541 gcaccgctcg cccgcttgag cgaagaggtc gcggcgcgca ccccggacac gctcgaccca
     3601 atcgcgaccg atcgggtgca aaaagagctc cgtccgctgg tggcggccct caatcattcg
     3661 atcgagcgtg tgcgcaacca gatcgtcgtg cagcggcgct ttatcgccga tgccgcgcat
     3721 cagctgcgca cgccgctgac tctcttgaag acacagatat tctatggcct gagcacggtc
     3781 gatctggacg agaagaacaa gtcgctctcg gcggtcgacg agagtgttgg ccagatggtc
     3841 catctcacca accagctgct gatgctcgcc aagatcgagc ccggcgccct gtcgatcgtt
     3901 cgcgagccgg tcgatctcgc cgcgtcggcg cggcaagtgc tcgaggcggt ggcggatcgg
     3961 gccctcgacc ggaacatcga gctcagcttc gatgcggcgc ccgtccgggt gctggccaat
     4021 cgcgccatgc tccacgagct catcatgaac atcgtcgaca atgcgcttcg ctacagcccg
     4081 accggcagcg ccatcaatgc ggcggtctcg gccggcgacg gcgtcggtat tctacgtgtc
     4141 gccgaccagg ggccgggcgt tcccgtgcac gagcgcgagc gcgtgttcga ccgcttctat
     4201 cggattcttg gcaccgacac cgagggtagc ggtctcggcc tctcaatcgt tcgggaaatc
     4261 gcctggcgct atggcggcac ggtgacgctc gccgattcgc ccctcggacc aggccttgtc
     4321 gtcgaggtga cgctgcccgc agcatccgag gcgtaggacc atcactcatt tcgcggcgtt
     4381 gacgaactcg tcggtgtagg tcttcgacag atcgatcgtc ttaccccgca aattctgcga
     4441 gaacccgctc aggaccgcga ggacggtggg cggcccgtcg atcggcatct ttccgtcggg
     4501 cgtgaagcgc gacttgctct gcgcgagtaa ttggatgtaa cccgatttat cgccctggaa
     4561 atattcgctc ggcatgtggt ccgcgatctc ggcggcgctg tgggtgtcga tgaagcggag
     4621 cgtcttgacg aaggcattgg ccaggcgctg cgccaaatcc cggtggcctc gtacccagga
     4681 ggattgaaca taaaggcagg ccgccggata tgtgcccccg agcgcctccc gggtcgcgtc
     4741 cggcgtccgc atgtcgacga ggatctcggc accgccggtc ttgagcagac gcgaggccgt
     4801 gggctccgtc gtcattcccg catcgatccg gccgtcgagc atagccgcga tgaacgtgtc
     4861 accggcgccg atcggcagca gagaaatgtc gcgcaacggc acgccatgct tcaccgccag
     4921 atactgcgtc aggaaatcgg tcgacgagcc caggccggtg acgccgagag tacgcccctt
     4981 gaaatcggca ggcgatctga tctccgtggc caggcgcgcg ggaatcagtt cgacctcgcc
     5041 gggcgtttgg ccgaagagaa cgatcgactc gatgaatttt ccgcgcgcct gaaggtcgat
     5101 cgcgtgatcg tagaagccga cgacgccgtc gaccgctccg gccaacagct cgtcctcggc
     5161 ctcgttgccg acgggctctg aacggatctc cacgtccaga tcctgctctt gaaacgcgcc
     5221 gagctgctcg ccgagagccg ccggcagata gatgagcttt tcgatgccgc caaccatcag
     5281 cgtgaccctg tcgccgccat gagcggcggt cgtcaccgtc gacagaagca tcgccaggac
     5341 catgcgaaac cgactggatt tcattgtagc cccctttaga gccgctaacc gaccggtcgg
     5401 aagcctcatg cgaaggcgcc cgcctccatc gcgcaccatc gatcgggcac aaggttgggt
     5461 cccggcttcg gcgagcgaaa cgcggtccat ggagcgtcga tcgagacgga catggggact
     5521 cgccgaatag cgtgatttga gcggaaattt caagctgcga gctgccgaga ttctgcaatc
     5581 gtccgatctt tcggcgcgat cgcactgctg ggcgagttga ttgctcgcag ggcgaaccgt
     5641 tcgcagcatg ggcagtctcc cggcagccgt gtcggccgct tatcgttctt cgatctcatc
     5701 gtagggcctg aaacctttca cgaagctgtc gagcggccgt attgccgctg tggcaactcg
     5761 cgacggctga cgagcgtcag ggcttgagga gcatgcgccc gaaagccttg tgctcgagaa
     5821 tgaagcgata ggcctgcgcc gcttcggcga gcgggaacac gcgatcgatc agaaccttcg
     5881 ggtcgccctt ggcgacgtcg tcgatccggc ccgcgaccgc ggtgaagctg cggttatgct
     5941 cctgagggaa cggttcggat gggtaaccat tagtgcgtgg tcttacccgg cgttatagct
     6001 cagctgaacc ggcgatccaa ggcgctccga cgggggcgga gcgggccggt cgaacttccg
     6061 ccttcgagaa ctgaaccatt tcggctggac gcccgaaatg ggcgcagagc ggaagtcttg
     6121 cgctgcttgg agataccgat tgggtggtgt gtgacccgac tgagccgcgg atgggtgcgc
     6181 gtgtctctaa ctgtaacggc ttgcaatcat ccgcgctcgg gatcgcaatc atcgcgccgg
     6241 cgggctcaca ttgattgcaa caggccgaaa cggccaggct caggatggtt gcaactgggc
     6301 tcacgatcat tgcaacggga tcgcaatcat cggccgctgg ctcacgctca ttgcaactgg
     6361 aaaccgggtt gcggagcgct gccgacgggg gtcgaaatta tcgccggcgc tgtcggcaca
     6421 gcgccagagc tgttcgcggg tgccgtcacg aacttcgctt gtcgactaaa gtcggtcggc
     6481 agcagcccga tcaggcgacg ccggaaccgg acattcaccc tctccctacc tcggcggcat
     6541 gttattcgga tccgaatccg agcgcctttc tctgtgacag agcatttcat cagatgcaca
     6601 acgaacacaa gcgcgagcat gacaacggcc ggcacccaga acacatcgaa ggaggcgagc
     6661 gaggatgcct gttgctgccg caggtttgcg agcgcctgca ctgccagttg ctgcgcggcg
     6721 acgggatcgc cggtctgctg caggagccag cgaggtccct acactgcagc cctcattgcg
     6781 caggaggctc ggagcgacta cgacgctgcc gattggaacg aagccccgta gaccgggagc
     6841 accccgcccc ggcttacctt gttctcgggc gaggcgagga agaggatcgt cgccgccacc
     6901 tcctccacct tgggccaggc atcgaaattg gccttgggaa agttgcggcg gttggtgggg
     6961 gtatcgatga tggagggtgc tacggcgttc accaggattc cctcccccgc cagttcctcg
     7021 cctagcgcct cggagagtgc ggcgacgccg gccttggcgg cggtagaggc gctcattgtc
     7081 gccccggtgc gcggctccag ggcctggcgc gccgccacgt tgacgatgcg cccgccggcc
     7141 ttcccacggc gcatcgccac caccgcctct cggcagcaga ggaagcagtt cacgagattg
     7201 tcgtcgatca accggcgcag cagctccccg ctggtatcgg tcagcggggc taaggcgaag
     7261 ccgccggcga catggatcga ggcccagagt gacgggaggc tgccatagag cttcaccacc
     7321 gccgcctcat ccgccagact cggcacgggg atcaggctca gcgccccggt ggggaaaccc
     7381 tccttctcct gctcctgccg atagggcaca tggcaatggg cacccgccgc cagcaatgcg
     7441 cccaccaccg cgcgccccag ggcgccggtt ccgccggtta tcaccacatg acgatcgcga
     7501 aaatccatgg gatcctcctg tttcctcagc ctcgatccgg cggtgttcgc gctgctcatc
     7561 ctgccgtctt cctctccagc ggcgccagga gcctgacgga tcgatgagtg ctcactcgtg
     7621 gaagcccttg ccgatggtcg ccttcttgtc ccggtactcc accttgaagc gcagcgtgcg
     7681 gatcaccacg cgagcttggg cggtcattcg catgaccacg ggcggcagcg cgggccacga
     7741 tgacactcgg gccgcagtaa gggaccggct atccgtcagc cccgcctgcg gtcgttctgc
     7801 cattattctc tcgctctcgc atgcgcgccc acccggccgc ccgcctagag gctcttcggc
     7861 tggctgtttc ggatcaggct cccgcctttg atcggcggag ccattcgtcg agaccgatgc
     7921 ggccgaggcg cgcttcgccc aacggcacga gcgagtgctc ctcgacgcgg ccgccgaagt
     7981 atcgggcctc ggggtcactc acgacctgac gcgggtcgcc gaccgccttc aggtagcggg
     8041 cgacgatttc gctgaacggg gcccgttccg ggccggcgat ctcgacgaag ccgtttcgtg
     8101 gcgcggcgag cgccagctcg gcaacgatgg cagcaacgtc gtccgccgcg atcggctgga
     8161 acaggccggg cgaaatcctg accatgtttc catctgcacc tgaatcggcg atgccgccga
     8221 ggaattccat gaattgggtc gcgtggatga tggtgtaggg gatgccggcg gcctcgatca
     8281 gcctctcctg ggcgaccttg gcgcggaaat agccaatgtc gggcgaccgg tcggctccga
     8341 cgatggatag cgcgacatgg tgccggacgc ccgctgcggc ctccgccggg agaaggttgc
     8401 gctccgaggt ctcgaagaat tccaacaccg ccttgtcttc gaatgagggc gaattggcga
     8461 ggtcgatcac cacctcagca ccggccagag cctccttgag cccctcgccg gtgatggtat
     8521 tgacgccggt attgggcgag gccgcgacgg cctcgtggcc gccctggcgc agaatggcga
     8581 cggtcttcgc gccgatcagg cccgtgccgc cgatgacgac gatcttcatg gcttgccctc
     8641 ccttgtcgac gacaggagcc ccttaaatgg gttgggatgc aagcgaggaa aatatctggg
     8701 cctcggcgta tcgcacctgc cttgagccga gcgtctgatt ccagtgcctc gcgacccagt
     8761 tcttggaatt cctcggcggc attgccgatt cagcgatgga tggaaataaa gtcaggcttt
     8821 cgcccggcct gttccagccc atcgcggcgg acgacgttgc cgccatcgtc gccgaggtgg
     8881 cgctcgcggc gccgcgaaac ggcatcgtcg agatcgccgg cccggaacgg gcaccgctcg
     8941 acgaaatcat cgcccgctat ctgaaggcgg tcggcgaccc gcgcgaggtg gtgtgtgatc
     9001 ccgaggcccg atactggggt ggccgggtcg aggagcgttc gctcgtaccc ttgggtgaag
     9061 cgcgcctcgg ccgcatcggt ttggacgaat ggctccgccg ctcacaggca agagcctgag
     9121 cccgcatccg ccaaccgaaa gatacggccc catgaaacgc gtcctctatg cgctgcttct
     9181 cgcctggcca cacgcatgcg aaatccgcgt tcatctacgc gaccgcgctt gagggggcga
     9241 tccgcagtca ggtcaacgac ggaccggtga cgacctataa ggcctgaccc gtcgagacaa
     9301 ccgaaacgcg gggtacgaat ggcggcgagg cagcccatcc cggtcgattg atcgatgaga
     9361 tcgcgagcac tttccgcctt ccggagcaga cggagtggga gcatggtcag gcaggtcccg
     9421 ctcatctttt ttatcttgac ggcatcactg tcggcgcgag ccgcaggatg agctcgttct
     9481 agacccaata aggaggatgc gtcgatggtc gagagtgata cggtggcgcg gagggagctc
     9541 aaatctgacg aactcagcaa aacacgcaga ccgcagaagc ggcacgagtt cgattgcatc
     9601 atcctgctac ttcaaggtgg cggtgccctc ggtgcttacc aggctggggt ctatgaggcg
     9661 ctagcggagg cagacctcca tcctaattgg gtcgccggga tctcaatcgg gtctatcaac
     9721 gccgcgatca ttgctggcaa ccctccggac aaacgcgtcg aaaaattgag ggagttctgg
     9781 gagggcatca ccaaccagcc cttcgacctc atcggcggcg aactgggctc ggtgatggcg
     9841 agaggggaag ttgcacgcac atcccttaat caactgagcg ccgccgtcgc tctccttacc
     9901 ggcgcttccg ggttcttccg accgcggatc ctgaatccgt ggtttcatcc gaccggcacg
     9961 atcgaggcga caagctacta cgacacgata cagctcaagg cgacgctcga gagcctcgtc
    10021 gatttcgacc gagtgaactc taacaacgcc aatcctcgtc tcagtctcgg tgccgtcaac
    10081 gtgcggagtg gtaatctgat ctatttcgac tcgtcgaggc aggctatcca accagagcat
    10141 gtgatggcga gcggcgcgct gccgcccggc tttcccgcga tcgagatcga gggcgagcac
    10201 tattgggacg gcgggctcgt ttcgaatacg ccgcttcagt gggttgctat gacgcaacgg
    10261 ccccccgacg cgctcgcctt ccaggtcgat ctctggagcg caaggggcga gtttccgcgc
    10321 aacctcgcag aggtggcgac ccggcaaaag gagatccagt attcgagccg gacccgcgcc
    10381 ttcaccgatt acttcaagcg tgtcattaag ctgcgtaaca ccgtcgcgga gctcctcgaa
    10441 gaactgccag aggaaatgcg gcagagcgaa aaagtgaaat tcctaaccgc gaacgtggaa
    10501 cggcac
//
LOCUS       DATGEM010000003         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_114116, whole genome shotgun sequence.
ACCESSION   DATGEM010000003 DATGEM010000000
VERSION     DATGEM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114116"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VKQ27_00065"
     CDS             complement(<1..419)
                     /locus_tag="VKQ27_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY87352.1"
                     /translation="MSFAANHPASRDQAKVMNRFDITKRMVARLKHDEAVIGGIGYTN
                     FDLWAAGRRPQNFYMLGSMGLAVPIALGVALAQPQRKVIALEGDGSILMQLGALATVA
                     ARGQKNLAIAIMDNGTYQITGGQLTSTEQGADIVAIAR"
     gene            complement(428..958)
                     /locus_tag="VKQ27_00070"
     CDS             complement(428..958)
                     /locus_tag="VKQ27_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY87353.1"
                     /translation="MDEEPSRTWHEIVRDVLKRHQVKLVTYVPDNVLRPLIEGVHADP
                     FFTAFSCAREEEAVGIVSGAWMGGLRGIVLMQTSGFATLPNAIASLAVPFQIPVLMMV
                     SERGTLGEFNIGQSLVARTMRPVLDCLAIEHHTMTRLDEAEFIVDRSITQAVATQAPC
                     VFILSPLLTGGKVFAK"
     gene            1070..2560
                     /gene="tcuA"
                     /locus_tag="VKQ27_00075"
     CDS             1070..2560
                     /gene="tcuA"
                     /locus_tag="VKQ27_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HLY87354.1"
                     /translation="MADAGPYDVIVVGAGNAALCAALAAQEQGAKVLVLEKASEEERG
                     GNSTFTAGGFRFVHHGVDDLRKDVLIDLSDAEAKQMLIPPFTTETYTEDLMRATEYLS
                     EPDLIDLLVGRSRETVVWMREKGVRWIPMFKRQSYMVNGVQHFFGGMAVEAVGGGWGL
                     VDFLFKSAERYGVTVRYDTGMRKLIQNRKGQVTGVTVFGPDGYEDISAKSVVLACGGF
                     EADPEMRVRYYGPGWELARVRGTRHNTGDGIKAALEIGAAAFGGWSTCHAVQWDLSAP
                     PFGDRVVLDNYQKHSYPIGIMVNANGERFVDEGADYRNYTYAKYGREVMKQPQRMAAQ
                     IFDNKTNHMTRDEYRIRQVTKAEANTIEELAKKLEISPEGLVKTVKEFNAACQPGEFN
                     PAVLDGKCTKGIAPPKSNWAIPIDEPPFHGYAVTCGITFSFGGLKITNEGEVEDTTGR
                     TIPGLFAGGELVGGIFYQNYLGGAGLMSGSVFGRIAGRSAGKFAKG"
     gene            complement(2561..>2817)
                     /locus_tag="VKQ27_00080"
     CDS             complement(2561..>2817)
                     /locus_tag="VKQ27_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87355.1"
                     /translation="GAGLDVWEDEPPPHDHRLMAFDNVLVSPHTAGATVESRDNMARF
                     AAEQLIDILDGKRPPRLINPEVWPAYRERFERILGVKPEG"
BASE COUNT          505 a          865 c          973 g          474 t
ORIGIN      
        1 cgcgcgatcg ccacgatgtc ggcgccttgc tcagtggagg taagctggcc gccggtgatc
       61 tgatacgtcc cattgtccat gattgcgatc gccaggttct tctggccacg cgcagcgacg
      121 gtggccaacg cgcccagctg catcaggatc gaaccgtcgc cctcgagcgc gatcaccttg
      181 cgctgcggct gcgccagtgc aacgcccaac gcgatcggca cggcgagccc catgctgccc
      241 agcatgtaga agttctgcgg ccggcgaccg gcggcccaca ggtcgaaatt ggtgtagccg
      301 atgccgccga tcacggcctc atcgtgcttc agtcgggcga ccatgcgctt ggtgatgtcg
      361 aaccggttca tcaccttggc ctggtcgcga ctggccgggt ggtttgctgc aaaggacatg
      421 gcggcgctca cttcgcaaaa actttgccgc cggtcagcag cggcgacagg atgaacacgc
      481 acggcgcctg ggtggcgacc gcctgggtga tgctgcgatc gacgatgaac tccgcttcat
      541 cgagccgcgt catcgtgtgg tgctcgatcg ccaggcaatc gagcacgggg cgcatggtgc
      601 gcgcgaccag cgactggccg atgttgaatt cgccgagcgt gccacgttcc gacaccatca
      661 tcagcaccgg aatctggaat ggcaccgcga gcgaggcgat cgcgttcggc agcgtcgcga
      721 agcccgacgt ctgcatcagc acgatgccgc gcagtccgcc catccaggcg ccggagacga
      781 tgcccaccgc ctcttcctca cgcgcgcatg agaacgccgt gaagaacggg tcggcgtgga
      841 cgccctcgat cagcgggcgc agcacgttgt cgggcacata ggtcaccagc ttcacctggt
      901 ggcgcttcag cacgtcgcgc acgatctcgt gccatgtgcg cgatggttct tcgtccattg
      961 agactcactc cgtgggagat ggccgggtca agcccggcca cgactgtgcg acgccttgcg
     1021 cccggcggca accgttcgta aggaggcggg atagaccagg agacccaaga tggctgatgc
     1081 cggcccgtat gacgtgatcg tggttggcgc gggcaacgcc gcgctgtgtg ccgctctggc
     1141 cgcccaggag cagggcgcca aggtgctggt gctggaaaag gccagcgagg aagagcgcgg
     1201 cggcaactcc accttcaccg cgggcgggtt ccgcttcgtc caccatggcg tggacgacct
     1261 gcggaaggac gtgctgatcg atctgtcgga cgccgaggcg aagcagatgc tgatcccgcc
     1321 cttcacaaca gagacctata ccgaggacct gatgcgggcg accgagtatc tctcggagcc
     1381 tgacctgatc gatctgctgg tgggccgttc gcgcgagacc gtggtctgga tgcgcgaaaa
     1441 gggcgtgcgc tggatcccga tgttcaagcg ccagtcgtat atggtgaacg gcgtgcagca
     1501 cttcttcggc ggcatggcgg tagaggctgt cggcggcggc tggggcctgg tggacttcct
     1561 gttcaagtcg gccgagcgct acggcgtcac ggtccgctac gacaccggca tgcggaagct
     1621 gatccagaac aggaagggcc aggtgacggg cgtgacggtg ttcggcccgg acggctacga
     1681 ggacatcagc gccaagtccg tggtgctggc gtgcggtggt ttcgaggccg atccggaaat
     1741 gcgggtgcgg tattatgggc cgggctggga gctggcgcgc gtgcggggca cgcggcacaa
     1801 caccggcgac ggcatcaagg cggcgctgga gatcggtgcc gcggcgttcg gcggctggtc
     1861 aacctgccat gccgtgcaat gggatttaag cgcgccgccc ttcggcgatc gggtggtgtt
     1921 ggacaactac cagaagcact cctaccccat cggcatcatg gtgaacgcca acggcgagcg
     1981 cttcgtcgat gagggtgcgg attatcgcaa ctacacctat gcgaagtacg gccgcgaggt
     2041 gatgaagcag ccacagcgca tggcggcgca gatcttcgac aacaagacca accacatgac
     2101 gcgcgacgag tatcgcatcc gccaggtgac gaaggcggaa gcgaacacga tcgaggaact
     2161 ggcgaagaag ctcgagatca gcccggaagg gttggtgaag acggtgaagg agttcaacgc
     2221 tgcgtgccag ccgggcgagt tcaatccggc ggtgctcgat ggcaagtgca ccaagggcat
     2281 cgcgccaccg aagtcgaatt gggcaatccc gatcgatgag ccgccgttcc acggctatgc
     2341 ggtgacctgc gggatcacct tcagcttcgg cgggttgaag atcaccaacg agggcgaggt
     2401 ggaggacacc accggccgca ccatccccgg cctgttcgcc ggcggcgagt tggtgggcgg
     2461 catcttctac cagaactatc tgggtggcgc ggggctgatg tccggctcgg tgttcggccg
     2521 gatcgcgggg cgttcagcgg ggaagtttgc gaagggctga tcagccctcc ggcttgactc
     2581 ccaaaattcg ctcgaagcgc tcgcgatagg ccggccagac ctcggggttg atcagccggg
     2641 gcgggcgctt gccgtccagg atgtcgatca actgctcggc ggcaaaccgt gccatgttat
     2701 cgcgagactc gaccgtcgcg ccggccgtgt gcgggctgac cagcacgttg tcgaaggcca
     2761 tcaaccggtg gtcgtgcggc ggtggttcgt cctcccacac atccagaccc gcgcctg
//
LOCUS       DATGEM010000004         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1027444, whole genome shotgun sequence.
ACCESSION   DATGEM010000004 DATGEM010000000
VERSION     DATGEM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027444"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VKQ27_00085"
     CDS             complement(<1..1410)
                     /locus_tag="VKQ27_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87356.1"
                     /translation="MMSLPPCRTGGAWRVWRLLLLLMLLGCLSLAPALADDQPPIPVR
                     LGTHPDFGRVVFDLSAKSDYRLMRDGQHVTIEFDKTGKIASAARGTRNVQSVAGGDGK
                     AELIIAPGAVLREQRFGGRVVIDVLDPPAGGLLPAPLQPALPPAAQPAAEAPPASQPA
                     ASPKPSQAALPMAMVVPEPSAPASSSKPAESPPAPPSQATIADATPAGPAAAQDAKAD
                     EKPQPSTLVVPFGGGGPVGAAAFRRGATAVVVFDRPIALDLSVLHDDPAFSTATAQTL
                     PAATVVVLRLNPATSLSVTSVPGAWRIAATAGEPEFKPIGATVADRRLLLSASAASTV
                     VTISDPINGGTLLVGTQRHSGQGMPVRRRTVEFSLLPTWLGVVVAPNSDMVTLRTVQD
                     GFLVINDPGGLALSPPSEMADELLHAAGQTHHFDFLEQPRDVALQRLSRLVVDDATAP
                     PLARGQRRQDVARALLSLGM"
     gene            complement(1407..2039)
                     /locus_tag="VKQ27_00090"
     CDS             complement(1407..2039)
                     /locus_tag="VKQ27_00090"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HLY87357.1"
                     /translation="MVDDTGDVLAQRLASIRARYPVSEPELVADVVRAVLMTMSGDLS
                     AQETSLLAEVEELGKTISSAKAEIAALRVDDITQAHIPFATDELDAIVDHTATATNAI
                     LSSCETLDEVAGSLSGEPAAKLQDATTRIYEACSFQDITGQRITKVVGTLKTIESKVA
                     QIIAAFGSGGPPIETPTEEAVGEAALLNGPQHPAVAMDQSDIDRLLASFE"
     gene            complement(2032..2418)
                     /locus_tag="VKQ27_00095"
     CDS             complement(2032..2418)
                     /locus_tag="VKQ27_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HLY87358.1"
                     /translation="MPIDKSMNVLIVDDYKTMLRIIRNLLKQIDFNNVEEATDGADAL
                     AKLRAGNFGLIISDWNMAPMTGLQLLQEVRADARLKGTPFIMVTAESKTENVVAAKQA
                     GVSNYIVKPFNAETLRDKIEKVLGHG"
BASE COUNT          459 a          858 c          801 g          459 t
ORIGIN      
        1 catgccgagc gacagcaaag cgcgcgctac atcctgacgt cgctgtccgc gagcgagcgg
       61 cggcgccgtc gcgtcatcca ccaccagcct gctgaggcgc tgcaacgcga cgtcgcgcgg
      121 ttgctcgagg aaatcgaagt ggtgggtttg gccagcggca tgcaagagtt cgtcggccat
      181 ctccgagggc ggcgatagcg ccagcccacc cggatcattg atcaccagaa acccatcctg
      241 caccgtccgc agggttacca tgtcgctgtt tggcgccacc accacgccaa gccaggtagg
      301 caacagcgaa aactccacgg tacgccgccg taccggcatc ccctgtccac tgtgccgctg
      361 cgtgccgacg agcaacgttc caccattgat cgggtcggag atggtaacaa ccgtgctcgc
      421 tgcagatgcc gatagcaaaa gacgccgatc ggcaaccgtc gctccgatcg gcttgaattc
      481 cggctcgcca gcagtcgccg cgatccgcca tgcacctggg accgaagtaa ccgacagaga
      541 cgtggccgga ttaaggcgga gcacgaccac ggtcgcagcc ggcagcgtct gggcggtcgc
      601 tgtgctgaac gccggatcgt cgtgcaaaac ggaaagatcg agtgcgatgg gccggtcgaa
      661 tacgacgacg gctgtggcgc cgcggcggaa tgcagctgcc ccgaccggac cgccgccgcc
      721 gaacggcacg accagcgtcg agggctgtgg cttctcgtca gccttggcat cctgcgccgc
      781 ggcgggtcct gccggagtcg cgtcggcgat ggtcgcctgc gacggcggtg caggtggcga
      841 ctctgcaggc ttcgacgacg atgccggcgc agaaggttcc ggcaccacca tcgccatcgg
      901 caacgctgcc tgcgatggct tgggagacgc cgcgggctgc gatgcgggag gcgcttcggc
      961 agcaggctgc gctgcgggag gcagcgcagg ctgtaacggc gcaggcaaga ggccgccggc
     1021 ggggggatcc aggacgtcga tcaccactcg gccgccaaag cgttgctcgc gcaacacagc
     1081 acctggcgcg atgatgagct cggccttgcc atcgccgccc gccacactct gcacattgcg
     1141 ggtgccacgc gcggccgaag caatcttgcc agtcttgtca aattcgatcg tgacgtgttg
     1201 accgtcccgc atcaggcggt agtcggactt tgccgacaga tcgaacacca cccggccgaa
     1261 gtccggatgc gtaccgaggc gcacggggat cggcggctga tcatcggcca atgccggggc
     1321 gagcgacagg cagcccaaca acatcagcaa cagaagcaga cgccagacac gccatgcacc
     1381 gcccgtcctg caaggcggca gcgacatcat tcgaagctgg ccagcaaccg gtcgatatcg
     1441 gactgatcca tcgcgaccgc cggatgctgc ggaccgttca gcagggccgc ctcgccaacc
     1501 gcttcctcgg tcggagtttc gatgggcggt ccgccagagc caaacgccgc aatgatttgc
     1561 gccactttcg attcaatcgt cttcaacgtc ccgaccacct tggtgatgcg ctggccagtg
     1621 atgtcctgga agctgcacgc ctcgtagata cgcgtggtgg catcctgcag cttcgcggca
     1681 ggctcgccgc tcaggctgcc tgcgacttcg tccagcgtct cgcaactgct caggatggcg
     1741 ttggtagccg tcgcggtatg gtcgacgatc gcatccagtt cgtcggtggc gaaggggatg
     1801 tgagcctggg tgatgtcatc cacgcgcagc gccgcgatct cggccttcgc actgctgatc
     1861 gtcttgccca gctcttccac ctcggcgagc aaggacgtct cctgcgcgga cagatcgcca
     1921 ctcatcgtca tcaacacagc gcgaacgacg tccgcgacca gctccggctc cgacaccgga
     1981 tagcgtgcgc gaatgcttgc cagccgttgc gcgagcacgt cgcccgtgtc atcaaccatg
     2041 tccaagcacc ttctcgatct tgtcgcgcaa cgtctccgcg ttgaacggct tgacgatgta
     2101 gttggaaacg ccggcctgct tcgccgccac cacgttctcg gtcttgctct cggccgtcac
     2161 catgatgaac ggcgtgccct tcagtcttgc atcggcgcgc acttcctgca gcagttgcag
     2221 tccggtcatc ggcgccatat tccagtcgct gatgatcagc ccgaaattgc cagcacgcag
     2281 cttggccaag gcgtccgccc cgtcggttgc ttcctcgacg ttattgaagt cgatctgctt
     2341 cagcagattg cggatgatcc gcagcatcgt cttgtaatcg tcgacgatga gcacattcat
     2401 ggatttgtct atcggcatgc ttgttcaatc ctttcaaccg ccacctgggt cattgcgccg
     2461 tgtgcgcatc tcggcgagtt ttgtggtgag gtcgcgagcc ttgtcgggct gcatggccgc
     2521 cagcaccggc gcggccttcg cctctttcat gcgatcgacg acctgcagca gcaccgg
//
LOCUS       DATGEM010000005         7199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_911555, whole genome shotgun sequence.
ACCESSION   DATGEM010000005 DATGEM010000000
VERSION     DATGEM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911555"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1876)
                     /locus_tag="VKQ27_00100"
     CDS             complement(<1..1876)
                     /locus_tag="VKQ27_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HLY87359.1"
                     /translation="MTKRMMIDATHAEETRVVVLDGNRLEDFDVETSTKRQLKGNIYL
                     AKVVRVEPSLQAAFVEYGGNRHGFLAFGEIHPDYYQIPVADRQRLLDMQAEDARLEEE
                     EEDRAAEQAAAEQAEGDSEPDGVEDIDSGVSPADEAFPTDSVVAAPATGEAPAPEAPA
                     EGPDVLAAPAEPAPAEAADQHAPHEPVAARPAEIVTEFVSAPTLFEVEETYEPATEPH
                     QPASEAEPVEATIEVLEAKPSTDELANEESDDTGILVEPILEQPPPETVGGDGDSNEY
                     NRERRIPPRFLRNYKIQEVIKRRQILLVQVVKEERGTKGAALTTYLSLAGRFSVLMPN
                     SPRGGGISRKITSATDRRRLKEIMAELDIPQGMGMIVRTAGANRPKPEIRRDCEYLLR
                     LWDDIREHTMKSVAPALIYEEASLIKRAIRDVYSRDIDEILVDGEDGWRAAHEFMRML
                     MPTHAKKVQLWRNGGQPLFSKYQVEAQLDAMLLPTAQLRSGGYLVINQAEALVAIDVN
                     SGRSTRERGIEETALRTNLEAADEVARQLRLRDLAGLIVIDFIDMESRKHNGMVERRL
                     KEALKNDRARIQVGHISHFGLMEMSRQRLRPSLAETSFIPCPHCGGTGHVRSTETAAV
                     H"
     gene            2473..3348
                     /locus_tag="VKQ27_00105"
     CDS             2473..3348
                     /locus_tag="VKQ27_00105"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLY87360.1"
                     /translation="MSSGSPISRRLLLGRLGSGSGIIGTFLLPGWMGSALAAPLQRTP
                     PARPVVKAPLPVVMLDPGHGGKDPGAIGVSGTYEKQVALAAAFELRRQLEATERYRVV
                     LTRTRDAFIPLEERVAKAQERGASLFVSMHADAIADRAVRGASVYTLSNHASDAQTAA
                     LAERENSADRFGGPEWHNAPPEVAHILAGLVRQETRVGSGQLARSLVGSLDRDVPMLP
                     NPERHAGFVVLKAADIPSVLVEMGFMSNPADEAELRRPQHRAMVAEAMKRAVEAYFAA
                     TAHVAELHHAERMAG"
     gene            3398..6049
                     /locus_tag="VKQ27_00110"
     CDS             3398..6049
                     /locus_tag="VKQ27_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HLY87361.1"
                     /translation="MSGPLTAGDKLAPPSPDNRKRRRQPTQPPRKPRRGVIRRIWRGF
                     LGAVLGVIVLVGAVGGVTGYVLYQRFSANLPDVDGLRNYRPPVMSRVYAGDARLMAEL
                     AAERRIFVPFTAIPDVVKQAFVSAEDQNFWTHPGVDPLAIARAGLFDVVHMGQGRRPL
                     GASTITQQVAKNMLLDAEVSLSRKAREAILAMRIEKALTKQRILELYLNEIYLGMGSY
                     GVAAAAQSYFNKPLDKLTLSEAAFLAALPKAPNNYNPFKYADAAKIRRDWVLDRMAED
                     HVITTQQASAAKAEPIVPAEFHRPPPIPGANWYVEEVRRELIARFGADVTTEGGLMVR
                     TSLDPALQAAAERSVRDGLMAYDRKLGGWRGPVSHIDAGGDFAKNWASALAQVTAPPG
                     MLPDWRLAVVLEETDGQARLGWLDPATGPAAGAPPQHLGVMSLSDVAWARPMKDGKLG
                     AVPHKMTDVVQPGDVVMVEPGGATGVAAKGAKPAPGAVSQPAPSQLALRQIPQVQGAL
                     VSLDPTTGRVLALVGGWSFEGSQFNRATQAQRQPGSSFKPFVYLTALEKGISPSQRFL
                     DAPIVMDTADGRWRPNNYEMEFGGPTPLRIALEKSLNLVTLRVAQTIGMEPIAQTAIA
                     FHEVENMPRVLPAAIGAVQTTVLREAGAYASIDEGGRLVEPTFIDSVQDPDGHVVWRP
                     NAIQCSNCGTAGQPPQLIDQRQQIADPASDFQIVTMMQGVTTRGTGAPVVAGLGHPIA
                     GKTGTTQDFTDAWFSGFTPDLVTTVWVGYDQPQSLGNNQTGAQVAGPIWHDFMAVALK
                     NRPVLQFQQPPGVTMAQWDTGSGVVTDAFKPDQVPGASRPIGSGLVSSTSTQDGGAVP
                     ASSDQSAQPQAGGNGGGGRDTGLGGLY"
     gene            6057..7188
                     /gene="prfB"
                     /locus_tag="VKQ27_00115"
     CDS             join(6057..6125,6127..7188)
                     /gene="prfB"
                     /locus_tag="VKQ27_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004873.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HLY87362.1"
                     /translation="MSAESDALNEQIKQSVALLRRHLDWDVAEGRLAELNARAEDPNL
                     WNDAAAAQKVMRERNQIAGQMEGVSKLEREVADTLELIGLAEADGDAAMAEEGLTALR
                     ALADEAKRREIESLLSGEADANDAYVEINAGTGGTEAQDWAEMLERMYSRWAEQHDYK
                     VEWIEESEGEQAGIKSATLRVNGPNAYGWLKTEAGVHRLVRISPFDSNARRHTSFASV
                     WVYPVVDDNIDIEVNENDLKVDTYRASGAGGQHVNKTESAIRITHVPSGIIVACQTDR
                     SQHRNRATAMAMLKARLYEAELQKREAAAAAVENAKTEIGWGHQIRNYVLAPYQLVKD
                     LRTGVEKGNPDAVLDGDLDAFMAAALAARAGATRSEASAQAR"
BASE COUNT         1196 a         2146 c         2586 g         1271 t
ORIGIN      
        1 catgcaccgc ggcggtttcc gttgagcgta catgccccgt gccaccacaa tgcgggcagg
       61 gaatgaagct ggtctcggcg agagagggac gcagccgttg gcggctcatt tccatcaggc
      121 cgaaatggct gatgtggcct acctggatgc gcgcgcggtc gttcttcagc gcctctttca
      181 ggcggcgctc gaccatgccg ttgtgctttc tcgactccat gtcgatgaag tcgatgacga
      241 tgaggccggc gaggtcgcgc aggcgcaact ggcgcgccac ttcgtcggcg gcttcgaggt
      301 tggtgcgcag cgcggtttcc tcgatgccgc gttcgcgggt cgagcggccg gagttcacgt
      361 cgatcgcgac aagggcctcg gcctggttga tcaccaggta gcctccagag cgaagctgcg
      421 ccgtgggcag cagcatcgca tcgagctggg cctccacctg gtatttgctg aacagcggct
      481 gcccgccgtt gcgccagagc tgcaccttct tcgcatgcgt cggcatcagc atgcgcatga
      541 actcgtgcgc ggcgcgccag ccatcttcgc catcgaccag gatttcgtcg atgtcgcgcg
      601 aatacacatc acggatggcg cgcttgatca ggctggcttc ctcgtagatc agcgcgggcg
      661 cgaccgactt catcgtgtgt tcgcgaatat cgtcccagag gcgcagcaga tattcgcagt
      721 cgcgtctgat ctccggcttc gggcggttgg cgccggcggt gcggacgatc atgcccatcc
      781 cttgcgggat atccagctcg gccatgatct ccttgaggcg gcggcgatcg gtagccgagg
      841 tgatcttgcg cgagatgccg ccgccgcgcg gtgagttcgg catcagcacg ctgaagcggc
      901 cggcgagcga gaggtaggtg gtgagcgctg cgcccttcgt gccgcgctct tctttcacca
      961 cctgcaccag caagatctgc cggcgcttga tgacttcctg gatcttgtag ttgcgcagga
     1021 agcggggagg gatgcggcgt tcgcggttgt attcgttgct gtcgccatcg ccgccgacgg
     1081 tttccggcgg gggctgttcg aggatcggct cgaccaggat gccggtgtcg tcgctttctt
     1141 cgttggcgag ttcgtcagtc gagggtttgg cctccagcac ctcgattgta gcttcgaccg
     1201 gttcggcttc ggaggccggc tggtgcggct cggtggcggg ctcgtaggtc tcttccacct
     1261 cgaacagggt gggtgcggag acgaattcgg tgacgatctc tgccggccgt gctgcgacgg
     1321 gttcgtgggg cgcgtgttga tcggcagctt cggcgggcgc aggttcggcg ggtgcggcca
     1381 aaacgtccgg accttcggcg ggtgcttccg gcgcaggtgc ctcgccggtg gcgggtgcag
     1441 caacgacgga atccgtcggg aacgcttcgt cggccggcga cactccgctg tcgatgtcct
     1501 cgacgccatc gggctccgaa tcaccctcgg cttgctccgc tgcggcctgt tcggcggcgc
     1561 ggtcttcttc ctcctcctcg agccgggcgt cttcggcctg catgtccagc aggcgctgcc
     1621 tgtcggcaac ggggatctga tagtagtcgg ggtggatttc gccgaaggcg aggaagccgt
     1681 ggcggttgcc gccatattcg acgaacgcag cctgcaggct tggttctaca cggactacct
     1741 tggcgaggta gatgttgcct ttgagctgtc gcttggtcga ggtttcaacg tcgaagtctt
     1801 cgaggcgatt accatccagc accacgacgc gggtttcttc cgcgtgcgtg gcatcgatca
     1861 tcatgcgctt tgtcatctag aactgaactc cggtaagccg ccccagggca cggcacggat
     1921 cccggtggga tgcgcgccgg cgggcagctg aataggcgtg tggaaagggg atggcccgtc
     1981 cgctcaagga tcggaggctg gtccggccga acgttacggc tgcttatcat cccgcatcta
     2041 aagatgggga tgaacgccga attggttaga gggtgccggc cgagcatcat gagttctgtc
     2101 gcaatctggg tccctggctt ccgggccgac gagggtccgg gggtgacgaa aactgcctga
     2161 cagcccgatc gcggcagccg aacccggcgc gctgggagcg cggcgcgggc gatgacgcgg
     2221 cagcggcgga ctccgaccct ccccggacct catggcgcca aactgcgtgt tcaccgcgca
     2281 cgagggccgt cgcctccgcc ggattccgtg cccaggagcg ggttggcccg ctttgccctg
     2341 agtgcgtcac cggagcaggc cagaccatga agcaaaccgg cgcgctccgc aagcgatggt
     2401 tggattaacc attaattagc cttaatcaac gcaaaaggtg caattctgca tccaatgtgt
     2461 taaagaaccc tcatgtcgtc cggttcgccg atctcccgcc gcctgttgct gggccgcctc
     2521 gggtccggat ctggcatcat cggtacattc cttctgcctg gctggatggg ttccgccctg
     2581 gcagcgccgt tgcagcgcac gccgccagcg cgtcctgtcg tgaaagcgcc gctccctgtg
     2641 gtgatgctcg atccggggca tggcgggaag gatccgggcg ccatcggcgt gtccggcaca
     2701 tacgaaaagc aggtggcgct cgctgcggca ttcgagttgc gacggcagtt ggaggcgacg
     2761 gaacgctatc gggtcgtgct gacccgcacg cgcgacgcct ttatcccgct ggaggagcgg
     2821 gtcgccaaag cgcaggaacg cggagcatcg ctgttcgtct cgatgcatgc ggatgcgatc
     2881 gccgaccgcg cggtgcgtgg cgcgagtgtg tatacactgt cgaaccatgc gtctgatgcg
     2941 cagacagcgg cgttggcgga gcgcgagaac agtgcggatc gattcggcgg gccggagtgg
     3001 cacaatgcgc cccctgaggt ggcgcatatc cttgccggcc tggtgcgcca ggagacgcgg
     3061 gtgggctcag ggcagttggc acgcagcctg gttgggagcc tggaccggga cgtgccgatg
     3121 ctgccgaatc cggagcggca tgcgggcttt gtcgtgctga aggccgcgga cattccgagc
     3181 gtgctggtcg agatgggctt catgtccaac ccggcggacg aagcggaact gcgccggccg
     3241 cagcatcgtg cgatggtcgc cgaggcgatg aagcgtgcgg tggaggcata tttcgccgcg
     3301 accgcacatg ttgctgaatt gcatcacgcg gaaaggatgg cgggctagcg tactgcacgc
     3361 tggtcgcgcc gcgaaaaacg tcccatatgg ctggcgcatg agcggtcccc ttactgccgg
     3421 cgacaagctg gcgcctccct cgccggataa ccgcaagcgc agacggcaac cgacgcagcc
     3481 accgcgcaaa ccacggcggg gcgttatccg ccgcatctgg cgcgggttcc tgggcgcggt
     3541 actcggggtt atcgtgctgg tgggggctgt cggcggcgtg accggttacg tcctctacca
     3601 gcgcttcagt gcgaatctgc cggatgtcga tgggctgcgg aactatcggc cgccggtgat
     3661 gagccgcgtc tatgcgggcg atgcgcgatt gatggccgag ctggcggcgg agcgccgcat
     3721 ctttgtgccc ttcacggcga ttcccgatgt ggtgaagcag gccttcgtgt cggccgagga
     3781 tcagaatttc tggacgcatc ctggggtcga tccgctggcc atcgcgcggg ctggcttgtt
     3841 cgacgtggtc catatggggc agggacgacg acctcttggt gcgtcgacca tcacccagca
     3901 ggtcgccaag aacatgctgt tggacgccga ggtgtcgctg tcgcgcaagg cgcgcgaggc
     3961 gattctggca atgcggatcg agaaggcgct gaccaagcag cgcatcctcg agctctattt
     4021 gaacgagatc tacctgggga tgggttccta tggcgtcgct gccgcggcgc aatcgtattt
     4081 caacaagccg ctcgacaagc tgaccttgtc ggaggcggcg ttccttgcgg ccctgccgaa
     4141 ggcgccgaac aactacaatc cgttcaagta tgcagatgcc gcgaagatcc ggcgggactg
     4201 ggtgctggat cgcatggcgg aagaccatgt gatcacgacg cagcaggcat cggccgcgaa
     4261 ggctgagcca atcgtgccgg ccgaattcca tcggccgccc ccgatccccg gcgcgaactg
     4321 gtatgtcgag gaagtgcggc gggagctgat cgcccggttt ggcgctgacg tgacgacgga
     4381 aggcgggctg atggtccgca ccagccttga tcccgctttg caggcggccg ccgagaggtc
     4441 ggtgcgcgac gggctgatgg cctatgaccg caagctgggc ggctggcgcg ggccggtctc
     4501 gcacatcgat gcaggcgggg actttgcgaa gaactgggcc agcgcattgg cccaggtgac
     4561 ggccccgccg gggatgctgc cggactggcg gctcgctgtt gtgctggaag agacggatgg
     4621 ccaggcccgg ctgggctggc tggacccggc caccgggccc gctgcggggg ctccgccgca
     4681 gcacctgggg gtgatgtcgc tctcggatgt cgcgtgggcg cggccgatga aggacggcaa
     4741 gctgggcgcc gtgccgcata agatgacgga cgtggtgcag ccgggcgatg tggtgatggt
     4801 agagccgggc ggcgcaacgg gcgttgcggc gaagggcgcc aagcccgcgc cgggtgccgt
     4861 atcgcagcct gcgccctcac agcttgcact gcggcaaatc ccgcaggtgc agggtgcgct
     4921 ggtgtcgctc gatccgacca ccgggcgcgt gctggccttg gtgggcggct ggagctttga
     4981 aggcagtcag ttcaaccgcg ccacccaggc gcagcggcag ccgggctcca gcttcaagcc
     5041 cttcgtgtat ctgacggcgc tggagaaggg gatttcgccg agccagcggt ttctcgatgc
     5101 gccgatcgtc atggatacgg cggatggccg ctggcggccg aacaattacg agatggagtt
     5161 cggtgggccc acgccgctgc ggatcgcgtt ggagaagtcg ctcaatctgg tgacgctgcg
     5221 cgtggcgcag accattggca tggagccgat cgcccagacg gcgatcgcgt tccatgaagt
     5281 ggagaacatg ccgcgtgtgc taccggcggc gatcggcgcc gtgcagacca cggtgttgcg
     5341 cgaggccggg gcgtatgcgt cgattgacga gggcggccgg ctggtcgagc cgacattcat
     5401 cgacagcgtg caggacccgg atggacatgt cgtctggcgg ccgaatgcca tccaatgctc
     5461 caattgcggc actgccggac agccgccgca gttgatcgat cagcgtcagc agatcgctga
     5521 tccggcgagt gacttccaga ttgtgacgat gatgcagggc gtcaccacgc gtggcaccgg
     5581 cgcgccggtg gtggccgggc tgggccaccc gatcgcgggc aagaccggca cgacacagga
     5641 tttcaccgac gcttggttct cgggcttcac gcctgatctg gtgacgaccg tctgggtcgg
     5701 ctacgaccag ccgcaatcgc tgggcaacaa ccagacaggc gcgcaggtcg ctgggccgat
     5761 ctggcatgac ttcatggcgg tggcactgaa gaaccgcccg gtgctgcaat tccagcagcc
     5821 gccgggcgtg acgatggcgc agtgggacac aggttcaggt gtagtgacgg atgcattcaa
     5881 gcctgaccag gtgccgggcg cgtcgcggcc gattggaagc ggattggtga gcagcacatc
     5941 cacgcaggac ggcggcgcgg taccggcctc atcggatcag tcggcgcaac cgcaggccgg
     6001 cggcaacggt ggcggcgggc gtgacaccgg gctgggcggg ctatattagg cgcgtcatgt
     6061 cagccgaatc agatgctctg aacgaacaga tcaagcagtc ggtcgcactg ctgaggaggc
     6121 atctttgact gggatgtcgc tgaaggccgg ctcgccgaac tgaacgcacg cgccgaggat
     6181 cccaacctct ggaatgatgc ggccgccgcg cagaaggtga tgcgcgagcg caatcagatc
     6241 gccggccaga tggagggcgt gagcaagctg gaacgcgagg ttgccgacac gctggaactg
     6301 attggccttg cggaggccga cggcgatgcg gcgatggccg aggaagggct gacggcgctg
     6361 cgtgccctgg cggacgaggc gaagcggcgc gagatcgaaa gccttctgtc gggcgaggca
     6421 gacgccaacg acgcctatgt ggagatcaac gccggcactg gcgggaccga ggcgcaggac
     6481 tgggccgaga tgctggagcg catgtattcg cgctgggcgg agcagcacga ctacaaggtg
     6541 gagtggatcg aggagagtga gggcgagcag gccggcatca agtcggcgac actgcgcgtc
     6601 aacggcccga atgcctatgg ctggctgaag accgaagccg gtgtgcatcg gctggtgcgg
     6661 atcagcccgt tcgattccaa tgcgcggcgg catacgagct ttgccagcgt gtgggtgtat
     6721 ccggtggtgg atgacaacat cgacatcgag gtcaacgaga acgatctgaa ggtcgatacc
     6781 tatcgtgcgt cgggcgcggg tgggcagcac gtcaacaaga ctgaaagcgc aatccggatc
     6841 acgcatgttc cgagcggcat catcgtcgcg tgccagacgg accgcagcca gcaccgcaat
     6901 agggcgaccg cgatggcgat gctgaaggcg cggctgtacg aggccgagtt gcagaagcgc
     6961 gaggcggccg cggcggcggt ggagaacgcc aagaccgaga ttggctgggg acaccagatc
     7021 cgcaattacg tgctggcgcc gtatcagttg gtgaaggacc tgcggaccgg ggtcgagaaa
     7081 ggcaatccgg acgcggtcct ggacggcgat ctggacgcgt tcatggcggc ggcgcttgcg
     7141 gcgcgcgccg gtgccacgcg cagcgaggct agcgcgcagg cacgatagct cttccgccc
//
LOCUS       DATGEM010000006         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_911545, whole genome shotgun sequence.
ACCESSION   DATGEM010000006 DATGEM010000000
VERSION     DATGEM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911545"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1530
                     /locus_tag="VKQ27_00120"
     CDS             121..1530
                     /locus_tag="VKQ27_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HLY87363.1"
                     /translation="MLFPTLDFLLFFVAVLAMLAPCTGLHEVRKLLLTAASYFFYAQW
                     NWHYCLLLAGSSLLTYAGGLAIDRASVQRTRQWSVGVTVGGHLLLLSTFKYLDFLVGS
                     ANHLLHAFGTGSELPFMEIILPVGISFFTFHGISYLVDVYRGDVAVCRRFVDILLYLS
                     FFPQLVAGPIVRASFFLPQLARPPLERVPIAEPVLLILGGLFKKVVIATYLATDLVDP
                     VFFDPSRYRPLDLVLAAYGYAVQIYCDFSAYTDMAIGFATLMGYRFPINFNQPYRAQS
                     LREFWQRWHISLSSWLRDYLYKPLGGSRGSRWFTARNLMITMLLGGIWHGAAWKFVAW
                     GALHGAGLVVERCLKPWDGNWARTRFGRLVAVLIVFHFVCLGWILFRADSFQTVVIYL
                     QTMVSGQGDESQVTPFSLALIVLGLWLHAVPRDMPARLARWFVAAPGWAWGVSAGVGI
                     AMIDALGPEGVAPFIYFQF"
     gene            1626..1868
                     /locus_tag="VKQ27_00125"
     CDS             1626..1868
                     /locus_tag="VKQ27_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87364.1"
                     /translation="MRVSPRHLLMLALLACVVAATLGAKPLAAWVDASLVSGTLVQGA
                     VDEWSDAMQRIGMTRPYDMLRHAIRDAEAARFPGRD"
     gene            2111..2368
                     /locus_tag="VKQ27_00130"
     CDS             2111..2368
                     /locus_tag="VKQ27_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87365.1"
                     /translation="MNDGPSAYTIPNLLHVRETVRGRTVYWLLPPHHEYARVAIRTVA
                     ARFGDRLIDCAPEAGPDGLHPTGAGYRTLGSLIQKPVRRAS"
BASE COUNT          348 a          676 c          793 g          559 t
ORIGIN      
        1 ctacgccgcg ccggatcacg tgcacatgtt tgcgccaggc tatcaggcaa cggcagaggc
       61 gctgttccgc agcatcatgg atggctacgc gcgctaccgg tcgttgttcc ctggtgcctg
      121 atgctgttcc cgacgctcga cttcctgctg ttcttcgtcg cggtgctggc gatgctggcg
      181 ccatgcacgg gactgcacga agtgcgcaag ctgctgctga ccgcggccag ctatttcttc
      241 tacgcgcagt ggaactggca ttactgcctg ctgctcgcgg ggagttcgct gctgacctat
      301 gcgggcggcc tggcgatcga tcgcgcatcg gtgcagcgaa cgcgccagtg gagcgttggc
      361 gtgacggttg gcgggcattt gctgctgctg tccacgttca agtatctcga tttcctggtg
      421 gggtcggcga atcatctgct gcatgcgttc ggcaccggca gcgaattgcc gttcatggaa
      481 atcatcctgc ccgtcggcat ctcgttcttc accttccacg gcatttctta tctggtggac
      541 gtgtatcgag gcgacgtcgc ggtgtgtcgg cggttcgtgg atatactttt gtatctatcg
      601 ttctttccgc agctggtggc cgggccgatc gtgcgggcgt cgttcttcct gccgcagctt
      661 gcacgtccgc cgctggaacg ggtgcccatc gccgaaccgg tgctgctgat cctgggcgga
      721 ttgttcaaga aggtggtgat cgcgacgtat ctagcgaccg atctggtcga tccggtgttc
      781 ttcgatccgt cgcgctaccg gccgctggat ctggtgctcg cggcatacgg ctacgcggtg
      841 cagatctact gcgacttttc cgcctatacc gacatggcga tcgggttcgc gaccttgatg
      901 ggctatcgct ttccgatcaa cttcaaccag ccttaccgcg cgcagagcct gcgcgagttc
      961 tggcagcgct ggcatatctc gctgtcgtcg tggctgcgcg attatctcta caagccgctg
     1021 ggcggcagcc gggggagccg gtggttcacc gcgcgcaatc tgatgatcac catgctgctg
     1081 ggcggaatct ggcatggggc ggcatggaag ttcgtcgcat ggggtgcatt gcatggcgct
     1141 gggctggtgg tcgagcgttg cctgaagccg tgggatggca actgggcgcg gacacggttc
     1201 ggccggttgg tggcggtgtt gatcgtgttc cacttcgtct gtctgggctg gatcctgttc
     1261 cgggccgaca gctttcagac cgtggtgatc tacctccaga cgatggtgtc ggggcaaggc
     1321 gatgagagcc aggtgacacc gttcagtctg gcgctgatcg tgctggggct gtggttgcat
     1381 gcggtaccgc gcgatatgcc ggcacgattg gcccggtggt ttgtcgctgc gccgggatgg
     1441 gcgtggggtg tctctgctgg ggttgggatc gcgatgatcg atgcgctggg acctgaaggt
     1501 gtggctccgt tcatttactt ccagttctga tgatgcatac gtcggtgcgg tcccctcctt
     1561 cccccagccc ccgggtcaag cccggggcgg ccagttccgc cccccaaggg agtggagatg
     1621 ggaatgtgcg cgtatcgcca agacacctgc tgatgcttgc gctgcttgcc tgtgtcgtgg
     1681 ccgcaacgct tggcgcgaag ccgctggccg cgtgggtgga tgcgtcattg gtcagcggca
     1741 cgttggtgca gggcgcggtc gatgagtggt cggatgctat gcaacggatc ggtatgacgc
     1801 gtccgtatga catgttgcgt catgcgatcc gtgacgccga ggccgcgcgg tttcccggtc
     1861 gtgattagat gttccttcac ctcgctgtga tacccaacac gcagcacgac gcggcgacgt
     1921 tttgtgtcgg ttgcttcaaa gggaggtcgg gttgattccg tgtttagcca ttggtgacag
     1981 tattgcaatt ggtgtcggtc atacgctccc tgaatgtcgc gtagaggcac gtgtcgggat
     2041 caattcgaag caattcatcc acgagatcct aacgccgcag caggcggata aggtagtcat
     2101 cagccttggc gtgaatgacg gcccgtcggc ttacacgatt ccaaatctgc tgcacgtgcg
     2161 ggagacggta cgtggccgca ccgtgtattg gctgttgccg ccgcaccatg aatatgcgcg
     2221 tgtcgcaatc cgtacagtcg cagcgcgctt cggcgatcgc ctgatcgatt gcgcgccgga
     2281 ggctggtcca gatggtctgc atccgaccgg tgcgggctat cgcacgctcg gttcgctgat
     2341 tcagaagccg gtccgccgcg ccagctagtc tattcc
//
LOCUS       DATGEM010000007         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1368421, whole genome shotgun sequence.
ACCESSION   DATGEM010000007 DATGEM010000000
VERSION     DATGEM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368421"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3159)
                     /locus_tag="VKQ27_00135"
     CDS             complement(<1..>3159)
                     /locus_tag="VKQ27_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87366.1"
                     /translation="IIPGQPPAGGLDLGGVLLGLIDGIGGQLAPSTAFATPLDHSATA
                     NPPVPAFVISEAQPLAGGSVTITNRSRDGGGAPLAAVLTVGDQASQALAANQSVSVTL
                     PTGITGVGIELATTDASGVRLSVVRSVAFGVPPTVTATPTSTNTPTATATVTPTATAT
                     PTMTPTLGPFTISRGDVLVGTFGGAVEIHAPDGSLKGIMHDPNPSFPEEMTGMAVDAD
                     SSVYVTEFESQRLIKFDHAGQFLGYIGNWSELGEPESIAFDAAGNAYVGVADGPGTIT
                     KLDPAGNVAMQFSAAEEERGTDWVDLEDDQCTLLYTSEGAFIKRFNVCANIQVADFNT
                     IPLSAPVFGGGAAAYALRILRDGGALVAHGTSILRLDSAGNVIQSYATPSVNNWYALS
                     LDPDCTSFWSGDFDSGNVYHFDIQSGAILSTFVIGDRFQRVTGVGVASECTAARSTPG
                     SVATVTPVPTPSQTPTPTATPTVTPTATTTPTATSVPPDFNLLVAPNTQGLPPGGSAA
                     MTVSLGSTDGFVSPVSLSVSGLPAGVTGTFDPPIVTPSGLSTLQLSAAADAPGTTGAV
                     PFTIAATGGGVTHSTQPSISVNFGLVPICYGAFTGVVTDASTGLPIPTAEVSLNYQFG
                     QPVDANGHFTFTNVALSAANGPMSYTIAADATNYKSDSANSATAVASCGSVTTVNFQL
                     HPQLFGSIGGTVFEGIPNPNDLSRTRTVTSSGTPLSGILVGGSQLTSADGGYLLQHLF
                     IFNDIFNVFGISASDTTNTFWPAQQVNVTVYPNQTTQHDFYLVRKCYGSLTVTLLDQA
                     TGQPIVGAFIQTGAQDGAPNPSSIQTDANGVARFTNAPLGYNNSQTTYGVSFGYDGIH
                     ESVSGQVNLVHCGDFAALTLLAKTVPPTPTPTATPVHFIATLQGTAYDLDTGAPISGL
                     GVTVARVFNSTCSSGLAGPTFTDSNGHYSLAVDLGTFSNTVLACAMGEPGLNDPPDLY
                     YFAYEAPDGSPYPAQVSPQLTLTAGATSTADIHIMKRRFGSVGGTVQDAATHQGVPFA
                     SVSISSQALYGNAGLDGSYRID"
BASE COUNT          611 a          921 c         1022 g          605 t
ORIGIN      
        1 gatcgatgcg gtagctgccg tccaggcctg cgttaccgta cagcgcctgc gatgatatcg
       61 acaccgaagc gaagggcacc ccctggtggg ttgcggcatc ctgcacagtg ccaccgacgg
      121 agccaaagcg acgtttcatg atgtggatat ccgccgtgct ggtcgcaccg gcagtgagcg
      181 tgagctgggg cgaaacctgc gccgggtatg ggctgccgtc gggcgcctcg tatgcgaagt
      241 aatacaggtc tggcggatcg ttcaggccag gctcgcccat cgcgcatgcg aggacagtat
      301 tgctgaatgt gccaagatca accgccagac tgtaatggcc attgctatcg gtgaacgtcg
      361 gtcccgccag gccgctgctg cacgtgctgt tgaagactct ggcgacggtg acacccagtc
      421 cagaaattgg cgcgcctgtg tccaggtcgt acgcagtgcc ctgcagcgta gcgatgaagt
      481 ggaccggcgt ggcagtcggc gtcggcgtcg gtggaacggt cttcgccagc aacgtgagag
      541 ccgcgaagtc gccgcagtgg accaggttca cctgtccact cacagattca tggataccgt
      601 cgtagccaaa ggagacgccg taggtcgttt gcgagttgtt atagccaagc ggcgcgttgg
      661 tgaacctggc gacgccattg gcatctgtct gtatggagga cgggtttggt gctccgtctt
      721 gtgcgcccgt ctgaatgaaa gcaccgacga ttggctggcc ggttgcctgg tcgagcagtg
      781 tgactgtgag cgatccgtag cacttgcgaa ccagatagaa atcgtgctgt gtcgtctggt
      841 tagggtagac cgtcacgttc acctgctgcg ccggccagaa cgtgttggtc gtgtcgctcg
      901 cgctgatccc gaacacgttg aagatgtcgt tgaagatgaa caggtgctgg aggaggtaac
      961 cgccgtcggc actggtcagc tgacttccgc ctacgagaat tccagacagc ggcgtgccgc
     1021 tggaggtcac ggtgcgcgta cgcgagaggt cgtttggatt ggggatgcct tcgaacaccg
     1081 tcccgccgat actgccgaac agttgcggat gcaattggaa attcacggtc gtgacgctgc
     1141 cgcagctcgc caccgcggtc gcagagttcg cgctgtcgga cttgtaattg gtcgcatcgg
     1201 cggcgatggt gtagctcatc ggaccattcg cagccgaaag agctacatta gtgaacgtga
     1261 agtgtccatt cgcatcgacc ggttgcccga actggtaatt cagcgaaacc tcggcggtgg
     1321 gaattggcaa accagtgctc gcatctgtga ccacaccagt gaatgcgccg tagcagatcg
     1381 ggacgaggcc gaagttgacg gagatactgg gctgggtgct gtgcgtgaca ccgccgcccg
     1441 ttgccgcgat ggtgaacggc accgcgccgg tggtgcctgg agcatccgcg gcagcactca
     1501 gttgcagcgt tgacaatccc gacggagtga ctattggagg atcgaacgtt ccggtcactc
     1561 cagcgggcag gccgctcacc gacaagctca cgggtgacac aaacccatcc gtggaaccga
     1621 gcgacacagt cattgccgcg ctgccaccag gtggaagccc ctgtgtattc ggcgcgacga
     1681 gcaggttgaa atcgggcgga acactcgttg cggtgggggt agtcgttgcg gtaggtgtaa
     1741 ccgtcggggt cgccgtaggc gtgggcgttt gagacggcgt aggcaccggc gtcacggtgg
     1801 cgacgctacc cggtgttgat cgcgctgccg tgcactcgct cgcgacccca acgccggtaa
     1861 cccgctggaa acggtcaccg atgacgaaag tcgagagtat ggcgccgctc tgaatatcaa
     1921 agtggtacac gttgccggaa tcgaaatcac cgctccagaa tgacgtgcag tcgggatcga
     1981 ggctgagcgc gtaccagttg ttcacagacg gcgtcgcgta ggactggatc acattgccgg
     2041 cggagtccag ccgcaggatg ctcgtcccgt gcgccaccaa cgcgccacca tcccgcagga
     2101 tgcgcaaggc gtatgctgcc gcacccccac caaacacggg agcgcttaat ggaatagtgt
     2161 tgaagtctgc tacctggata ttcgcgcaga cgttaaagcg cttgatgaaa gcaccttctg
     2221 acgtgtagag cagcgtgcac tggtcgtcct ccaggtcaac ccagtcagtg ccacgctcct
     2281 cttccgcggc actgaactgc atcgccacgt ttccggccgg atcgagtttg gttatggtcc
     2341 ctggaccatc ggcgacgcca acgtacgcat taccggccgc gtcgaacgcg atcgattcgg
     2401 gctcacccag ctcggaccaa tttccgatgt acccaagaaa ctggccagca tgatcgaatt
     2461 tgataagtct ctgcgactcg aactcggtga cgtagacact ggaatccgcg tccacagcca
     2521 tgccggtcat ttcctcgggg aagcttggat tcgggtcgtg catgatgccc ttcaaacttc
     2581 catccggtgc gtgaatttcc accgccccgc caaacgtccc caccagcaca tcacctctgc
     2641 tgatcgtgaa tggcccaagc gtgggcgtca tggttggagt agccgttgcg gttggagtga
     2701 cggtggctgt tgccgtgggc gtattggtcg atgtcggcgt tgcagttacg gttggaggga
     2761 cgccgaatgc cacactgcgc accaccgaaa gacgcacacc ggacgcatcc gtcgtcgcga
     2821 gttcaatacc gacaccggtg atgcctgtag gcaatgttac tgagacgctc tgattcgccg
     2881 caagtgcctg ggatgcctgg tcacccacgg ttagcaccgc cgcgagcggg gcgccgccgc
     2941 catcgcgcga gcggttggtg atcgtcactg acccgccggc gagcggttgc gcttccgaaa
     3001 tgacgaacgc gggcaccggt ggattggcag tggcggagtg atcgagaggc gtcgcgaacg
     3061 cggttgacgg agcgagctgc ccgccaatgc cgtcgatcaa cccgagcaac acaccgccga
     3121 ggtcgagccc gccggccggc ggctggccgg gaatgatcg
//
LOCUS       DATGEM010000008         6910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1598754, whole genome shotgun sequence.
ACCESSION   DATGEM010000008 DATGEM010000000
VERSION     DATGEM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598754"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VKQ27_00140"
     CDS             complement(<1..913)
                     /locus_tag="VKQ27_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HLY87367.1"
                     /translation="MLALGEEQCTRLFSMMHEDEIKDISSAMSQLGAIKADVVERLCV
                     DFVDHMGAAGNLVGSYESTERLLLKSLPRDRASQIMEEIRGPAGRTMWDKLGNVHEAV
                     LANYLKNEYPQTVAVVLSKVKPDHAARVLSLLPDSFAMEVVMRMLRMESVQKDVLDGV
                     ERTLRMEFMSNLARSTRRDAHEMMADIFNNLDRQAETRFLAALEERNREAAERIKALM
                     FTFDDLLRLTPQAVQVLLRAVEKDKLPIAMKGASERLRDMFFKNMSERAGKILKDDIE
                     ALGPVRLRDVDEAQASIVVVAKELAAQG"
     gene            complement(972..2624)
                     /gene="fliF"
                     /locus_tag="VKQ27_00145"
     CDS             complement(972..2624)
                     /gene="fliF"
                     /locus_tag="VKQ27_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HLY87368.1"
                     /translation="MKALLEGLRALGPARLAAMGAVTIGMLGMLALMMLRTGTEPMAL
                     LYGDLDLRDSAQVVDQLQRQHIPYRVAGNGSQILVAANQVADARLGLAKAGLPNSGSV
                     GYEIFDRSDGFAATEFQQQINATRALEGEIARTLTAMHGVRSVRVHLVLPRREPFARD
                     RQDAQASVMLTMAGAARLDREGVQAILNLIAAAVPGLRPQNIAIVDSRGDLLARAGEP
                     VGAAATAMSTEEVRRGTELRLARAVEEMLERSVGAGHVRAEAAVRMSFDKVNETQERY
                     DPDGQVTRSTQTVNSTSKSTEPAPTVSVQNNLPNADAGQQSAGSQEGRQEETTNYEIS
                     KSVRTLIKDQPQIDRISLAVIVDGTASVGPDGKQAWQPRSAEELDRIASLVKTAIGYD
                     EKRGDHVEVASMRFVTEVTAPPEVNGILAMKLEKADLMHLAQTVLFGLIGIVALLLVL
                     RPMVLRITAAAPGAIGGGGGAFAALAGPGGMPAVAGAGVPSALAGPALLPPLLEDESM
                     VKIAQIEGQMRASSLRRITELVEKHPEETLAIVRGWMVQESG"
     gene            2823..4100
                     /locus_tag="VKQ27_00150"
     CDS             2823..4100
                     /locus_tag="VKQ27_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HLY87369.1"
                     /translation="MKSSRMTTYILVAMLLGIGIGGIIHDEFAAPATQKLIASYFSLG
                     SMIFLRLIKMIIGPLVFSTLVVGIGHMSDAGTVGRVGGKAMLWFVCASIVSLALGLVL
                     VNAFDPGFGVHKVVAGVNSGIDAHAMTLDGFIKHVFPDSAVRPLVENEILQIVVFAIF
                     FGVACAAMGERARIVVDTIEGVSHIILRVTGYIMKLAPFAVLCAMASTIATNGLGILV
                     NYAKFMGEFYFGLLLLWILLVVVGFVFLGPSIKRLILLVREPFLLAFSTASSEAAYPK
                     MLEQLAKFPISRNISSFVLPLGYSFNLDGTMMYCTFASVFIAEAYDIPLSWGTQIAML
                     LTLMLTSKGVAGVPRASLVVIAATLNQFSLPEEGLLLILGIDTFLDMGRSATNVIGNS
                     LATAVVAKWEGQLIADLDTIEMIEEMEVEPVPT"
     gene            4097..5023
                     /locus_tag="VKQ27_00155"
     CDS             4097..5023
                     /locus_tag="VKQ27_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HLY87370.1"
                     /translation="MIRLLLAAALALSAACGSARADDDTALTGTLKTINARGTILIGY
                     RTTSLPFSFLNKAGQPIGFSVDICHGIAEDVARALHRDLIEADAPAWQTGVRIVYVPV
                     AADERLPKVVSGAIDLECGSTTANAERGKTVAFSPVFFLSGTKLLVPAKSGITSYRGL
                     AGRTVVVGAGTTNADVMRKLANRVSPPLNVVEAPNLDAAYAMLVAGKADAFASDDILL
                     AGYVATHPEGHGFTIAGDYLSFEPYAITLRKDDPAFSDLVKDSFTRMASEGTLGRLYD
                     RWLIHRLPTGETMGVPMNPYLTEMYRALGQPD"
     gene            5246..6112
                     /gene="dapA"
                     /locus_tag="VKQ27_00160"
     CDS             5246..6112
                     /gene="dapA"
                     /locus_tag="VKQ27_00160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLY87371.1"
                     /translation="MLQGVWLPLITPFHNGAVDEASLARLAAHFATEVDGFILAATTG
                     EGMVLDDDETERVIRTAAKEIGGRRPVFLGLCGSDTRRLEQRLRATVEWPVDGYLITC
                     PYYSRPSQEGMFRHFSLLADATDRPVLVYNIPYRTGVNLANATLLRLAAAMRNIVGVK
                     DCCADMAQTHDLLHHRPADFAVLTGEDAQFFQAMAAGANGGILASAHTVPAAFAAVAN
                     AIMDGRLSDARAQWTRLIDLVRLLFAEPNPAPIKHLLWRRGLIASPELRLPMTGISTP
                     LADRLEAWVAPD"
     gene            6178..6735
                     /locus_tag="VKQ27_00165"
     CDS             6178..6735
                     /locus_tag="VKQ27_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87372.1"
                     /translation="MLDLDRFFADCQAAVAADPSHRLVKDVVTRAVSDPGGVMAAVGE
                     PERAGINVIFRAPNLTILNLIWGPGMTIMPHDHRMWAVIGIYTGREDNMFWRRLPSDA
                     GRDIEAAGAKSLCRGDCTPLGADIIHSVTNPIQRLTGAIHVYGGDFLTQERSEWDPES
                     LHERPYDAAKARKLFEDSNRILEAA"
BASE COUNT         1198 a         2307 c         2064 g         1341 t
ORIGIN      
        1 cgccttgcgc cgcaagctcc tttgccacca ccacgatgct ggcctgcgct tcgtccacat
       61 cgcgcagcct taccgggcca agtgcctcga tatcgtcctt caggatcttg ccggcacgct
      121 ccgacatgtt cttgaagaac atgtcgcgca gcctctctga tgcacctttc atcgcaattg
      181 gcagcttgtc tttctccacc gcgcgcagca gcacctgcac agcctgcggc gtgagccgca
      241 gcagatcgtc gaaggtgaac atcagcgcct tgatgcgttc cgccgcctcg cggttgcgtt
      301 cctccaaggc ggcgaggaaa cgcgtctcgg cttgacggtc caggttgttg aagatgtccg
      361 ccatcatctc atgcgcatcg cgccgcgtgc tgcgcgcaag attggacatg aattccatgc
      421 gcaatgtgcg ctcgaccccg tcgagcacgt ccttctgcac actctccatg cgcagcatgc
      481 gcatcacaac ttccatggcg aacgagtctg gcagcaaact cagcacgcgc gccgcatggt
      541 ccggcttcac cttggacaga acgaccgcga ctgtctgcgg gtattcgttc ttaaggtagt
      601 tcgccagcac tgcctcgtgc acattgccga gcttgtccca catggtgcgt ccggcgggac
      661 cgcggatttc ctccatgatc tgcgacgcgc ggtcccgcgg cagagacttc agcaacagcc
      721 gctcggtgct ttcatagctg ccgacaagat tgccggcggc acccatatgg tcgacgaaat
      781 cgacgcacaa ccgctccacc acatcggcct tgattgcgcc cagctgcgac atcgcggacg
      841 agatgtcctt gatctcatcc tcatgcatca tggagaacag ccgcgtgcac tgctcctcgc
      901 ccagtgcgag catcagtatt gctgccttct gcgcaccgct gatgccgcga tagtcctcgg
      961 atttcagcac ctcaaccgct ctcctgcacc atccagccgc gcacgattgc caaagtctct
     1021 tccggatgct tttccaccag ttcggtgatc cggcgcagtg aggacgcacg catctgtccc
     1081 tcgatctgcg cgattttcac catgctttcg tcttccagca gcggcggcaa cagtgccggt
     1141 ccggccaacg ccgacggcac gcccgcacct gccaccgctg gcattccgcc ggggccagca
     1201 agcgccgcga atgcgccacc gccaccgccg atggctccag gcgctgccgc ggtgatccgc
     1261 agcaccatcg gccgcagcac cagcagcaac gcaacgatcc cgatgagccc gaacagcacc
     1321 gtctgcgcga gatgcatcag gtctgccttt tccaacttca tggcgagaat gccgttgacc
     1381 tcgggcggag ccgtcacctc ggtgacgaag cgcatgcttg ccacctcgac atggtcgccg
     1441 cgcttctcgt catagccgat ggccgtcttg accaggcttg cgatgcgatc cagttcctcc
     1501 gcggagcgcg gctgccaggc ctgcttgccg tcggggccaa cgctggcagt gccgtccacg
     1561 atgactgcga gactgatgcg atcgatctgc ggctgatcct tgatcagcgt acggactgat
     1621 ttgctgattt cgtaattggt ggtttcctcc tgccgtcctt cctgggagcc cgcactctgc
     1681 tggccggcat cggcattcgg cagattgttc tgaaccgaaa cggtcggcgc aggctctgtg
     1741 ctcttcgacg tgctgttcac cgtctgcgtg ctgcgcgtca cctggccgtc ggggtcgtaa
     1801 cgctcctgcg tttcattgac cttgtcgaag ctcatccgca cagccgcctc ggcacgcaca
     1861 tgcccggcgc ccacactgcg ctccagcatc tcttcgacgg cgcgtgccag ccgcagttcg
     1921 gtgcctcgcc gcacttcctc ggtggacatc gcggtcgcag ccgcacccac gggttcgcca
     1981 gcacgcgcca gcaggtcgcc gcgcgagtca acgatcgcga tattctgtgg ccgcaggccc
     2041 ggcaccgcag cggcaatcag attgaggatc gcctgcacgc cttcgcgatc cagccgcgcc
     2101 gcgcctgcca tggtcagcat cacgctggcc tgcgcgtcct gtctgtcacg ggcgaacggc
     2161 tcgcggcgcg gcagaaccag atgcacccgc acagagcgta ccccatgcat ggcagtgagg
     2221 gtgcgcgcga tttcgccctc gagcgcccgt gtcgcattga tctgctgctg aaactccgtg
     2281 gcggcgaaac catccgagcg atcgaaaatc tcgtagccaa cggagccgct gttgggcaga
     2341 ccggccttcg ccagaccaag ccgtgcgtcc gcgacctgat tcgccgccac taatatctgg
     2401 ctgccattgc ccgccacacg atatgggatg tgctggcgct gaagctggtc aaccacctgg
     2461 gccgaatcgc gcagatcaag gtctccgtaa agcagcgcca tcggctcggt gccggtgcgc
     2521 agcatcatca gcgcaagcat gccaagcatg ccgatcgtca ccgcgcccat cgcggcgagc
     2581 cgtgctggcc caagggcccg cagtccctcc aggagggcct tcattgttca gacacctcta
     2641 cgttgaaaaa gagaatggcg catcgcgctt accccgccgt taacaggcgc ggcagaatct
     2701 gccgggtggc gagattacgc gcgacgatcc ggccctcagc cggagcaaaa ttaccattgc
     2761 gtgtctacgt ggttccccgc aacataatcg tcttacgcgt cgcagcaggg gagccgcacg
     2821 taatgaagtc cagtcgtatg accacgtata tcctcgttgc catgctgctc ggcattggca
     2881 tcggcggcat cattcatgac gagttcgctg ccccggcaac ccagaagctt atcgccagct
     2941 acttctcgct cggttcgatg atcttcctgc ggctgatcaa gatgatcata ggcccgctgg
     3001 tcttctcgac gctggtcgtc ggcatcggcc acatgtcgga cgcgggaacc gtcggtcgcg
     3061 tcggcggcaa ggcaatgctg tggtttgtct gcgcctcgat cgtgtctctg gcactgggcc
     3121 tggtcctggt gaacgccttt gacccgggct tcggcgtgca caaggtcgtc gccggagtca
     3181 attctggcat tgacgcgcat gccatgacgc tcgacggctt catcaagcat gtcttccccg
     3241 attcggccgt ccgtccgctg gtggaaaacg agatcctcca gatcgtcgtg tttgcgatct
     3301 tcttcggggt tgcctgcgcc gcgatgggcg agcgtgccag gatcgtggtg gacacgatcg
     3361 agggcgtatc gcacatcatt ctccgcgtca ccggctacat catgaagctg gcgccgttcg
     3421 cggtgctgtg cgccatggcg tccacaatag ccaccaacgg cctcggcatc ctggtcaatt
     3481 acgccaagtt catgggcgaa ttctacttcg gcctgctgct gctgtggatc cttctcgtcg
     3541 tcgtcggctt cgtcttcctc ggacccagca tcaagcggtt gatcctgctg gtgcgtgagc
     3601 cgttcctgct cgcgttctcc accgcgagct ccgaggcagc ctatccgaaa atgctcgaac
     3661 agctcgccaa gttcccgatc tcgcgcaaca tctccagctt cgtgctgccg cttggctatt
     3721 ccttcaacct cgacggcacg atgatgtact gcaccttcgc cagcgtgttc atcgccgagg
     3781 cttacgatat cccgctgagc tggggcacgc agatcgcgat gctgctgacc ttgatgctga
     3841 cctcgaaggg cgtcgccggc gtgccgcgcg cctccctggt cgtgatcgcg gccacgctga
     3901 accagttcag cctgccggag gaagggctgc tgctgatcct tggcatcgac acgttcctcg
     3961 acatgggccg gtcggccacc aacgtcatcg gcaactcgct ggcgaccgcg gtggtggcga
     4021 agtgggaagg ccagctcatc gcggatctgg atacgatcga aatgatcgag gaaatggagg
     4081 tggagccggt gcccacatga tccggctgct tctcgccgcg gccctggcat tgtcggccgc
     4141 atgcggcagc gcgcgggcgg acgacgacac cgcgctgacc ggcacgctga agacgatcaa
     4201 cgcgcgcggc acgatcctga tcggctatcg cacgacctcg ctgccgttct cctttctgaa
     4261 caaggcaggc cagcccatcg gcttctcggt cgatatctgc cacggcatcg ccgaggacgt
     4321 ggcgcgcgcg ctgcaccgtg acctgatcga ggcggatgca cccgcatggc agaccggcgt
     4381 gcgcatcgtc tatgtgccgg tcgccgcaga tgaacggctg ccgaaggtcg tatccggtgc
     4441 gatcgacctg gaatgcggct ccaccacagc gaatgccgaa cggggaaaga ccgtcgcttt
     4501 ctcgccggtc tttttcctga gcggcaccaa gctgctggtg ccggccaaga gcggcatcac
     4561 atcctatcgt ggcctggcgg ggcggacggt ggtcgtcggc gccggcacga ccaacgccga
     4621 cgtgatgcgc aagctggcga accgcgtgtc accgccgctc aacgtggtgg aggcaccgaa
     4681 cctggatgcc gcctatgcga tgctggtggc aggcaaggcg gatgcgttcg cctccgacga
     4741 catactgctc gccggctatg tcgccaccca ccctgaaggc cacggcttca ccatcgcggg
     4801 cgactacctg tcgttcgagc cctacgccat cacgctgcgc aaggacgatc ctgcattctc
     4861 cgatctcgtg aaggattcct tcacccggat ggcgagcgag ggcacgctcg gacgcctcta
     4921 cgatcgctgg ctcatccaca ggctgccgac aggcgaaacc atgggtgtgc cgatgaaccc
     4981 ctatttgacc gagatgtatc gcgcgctcgg gcagcccgac tgataccagt cggttgacat
     5041 ccgcatcggc acacgacagc atcgttgcgt gtacagtggc acctccttca tgggacgaaa
     5101 gtctggctcc tccggggccg aggaacgctg cgtgcactga gcgcgtggac taatcactca
     5161 ccaatggccc cgccaactcg gacggggcct ttttcttatc tgcttcttcc gaaccagcgg
     5221 cgtcgcaaca aaggaagaag cacaaatgct gcaaggagtt tggctgccgc tgatcacccc
     5281 gttccacaat ggcgcggtgg acgaggcatc tctggcgcgt ctggccgcac atttcgcaac
     5341 cgaggtggat gggttcatcc tggccgcgac caccggcgaa ggcatggtgc tcgatgacga
     5401 cgagaccgag cgcgtcatca ggactgcggc aaaggagatc ggtggacgca ggccggtctt
     5461 tctggggttg tgcggcagcg acacgcgccg gctcgagcag cggctgcgtg ccaccgtcga
     5521 gtggccggtg gacggctatc tgatcacctg cccgtactac tcgcggccct cgcaggaagg
     5581 gatgttccgg catttcagcc ttctcgccga tgccaccgat cggccagtgc tggtctacaa
     5641 cattccctat cgcaccgggg tcaatctggc gaatgccacg cttctgcggc tggccgcagc
     5701 gatgcgcaac atcgtgggtg tcaaggactg ctgcgccgac atggcgcaaa cccatgacct
     5761 gcttcaccac cgtcccgcag atttcgcggt gctgaccggt gaagacgccc agttcttcca
     5821 ggccatggcc gccggcgcga atggcggcat cctcgcgtcg gcgcatactg ttcctgcggc
     5881 gtttgccgcg gtcgccaatg ccatcatgga cggacggctg tccgatgcac gggcacagtg
     5941 gacccggctg attgatcttg tgcgcctgtt gttcgccgaa ccgaacccgg cgccgatcaa
     6001 gcatctcctg tggcggcgcg gcctgatcgc ttccccggag ctccgcctgc cgatgacagg
     6061 gatcagtaca cccctggccg accggctgga ggcctgggtc gcaccagatt gatcttgctc
     6121 ccgaagtggg aatcgaggat agttttcttc catcgattac ctcacaggga gaagactatg
     6181 ctcgacctcg accggttctt tgccgattgc caggcggccg tcgcggccga tcccagccat
     6241 cggctggtaa aggacgtggt gacgcgggcg gtcagcgacc ctggtggcgt gatggcggcc
     6301 gtgggggagc cggagcgtgc cggcatcaat gtgattttcc gcgcgccgaa cctgacgata
     6361 ctgaacctga tctggggacc cggcatgacg atcatgccgc acgaccatcg gatgtgggcg
     6421 gtgatcggta tctacaccgg ccgcgaggac aacatgttct ggcgccgcct cccctccgac
     6481 gccggccgcg acatcgaggc agccggtgcg aagtcgctct gccgtggcga ctgcacgccg
     6541 ctgggcgccg acatcatcca ctcggtgacc aatcccatcc agcgtctcac cggcgccatc
     6601 catgtctacg gtggtgactt cctgacgcaa gagcgcagcg agtgggatcc ggagtcgctg
     6661 cacgagcgcc cgtatgacgc ggcgaaagca cggaagctgt ttgaggacag caaccggatc
     6721 ctcgaagccg cgtgaccgcg cagctccccg cataaggctg ccaaactcag acgaagaact
     6781 tgctgacctg ggtggcgaac tgcaccagtt cgttcagcaa cctgagaaac tgctggatct
     6841 cctgcagtgt atccagtttg ctcctgatct cgttggtcac agcgtcgagc ttcttctgaa
     6901 tgtcggcgac
//
LOCUS       DATGEM010000009         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_342193, whole genome shotgun sequence.
ACCESSION   DATGEM010000009 DATGEM010000000
VERSION     DATGEM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342193"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..426)
                     /locus_tag="VKQ27_00170"
     CDS             complement(85..426)
                     /locus_tag="VKQ27_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HLY87373.1"
                     /translation="MIKVVGLLTRKPGLTHEQFVKHWLEIHGPLAHAVPGVRRYVQSH
                     IIGTRTRPDIPETDVEVDGIAELWYDDEAALTRAAATPEMKRLTDDGALFIGQIKSYV
                     IEEKQIIPPQR"
     gene            complement(423..1316)
                     /locus_tag="VKQ27_00175"
     CDS             complement(423..1316)
                     /locus_tag="VKQ27_00175"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87374.1"
                     /translation="MTASIRAIDPVARPFFAGEVSGIDITQPLSHAEAAAIAAGMDQY
                     GVLVFRDQHFTDETQLAFSRNFGALEQATGDIAQGSERRLSMDVNDISNLNKNNEILA
                     RDDRRRLFGLGNRLWHSDSSFKAVSAKYSLLSARRIPSAGGNTEFADMRAAYDALDDE
                     TKAECENLICEHSQLFSRAVLGFSDFTDEERQKFAPVRQRLVRRHPVTGRRSLFLASH
                     AGAILGWPVPEARAFLRDLTEHATQRKFVYSHVWKQWDLVMWDNRVTMHRARPFDNAE
                     VRDMHRTTVACELSTMDHQAA"
     gene            complement(1375..>2859)
                     /locus_tag="VKQ27_00180"
     CDS             complement(1375..>2859)
                     /locus_tag="VKQ27_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87375.1"
                     /translation="RPLNEIIGAGVRIHAPYTHVPYHERIDDGYPNFVNNDHCLLSAR
                     ATVNLAKMVPQDLAMLPMAQTIWYIPSGLDIWNQKIDKAPGHYTRMRGKLGTVEKPPE
                     PVVYWPDQEPLRQDGPLKERLNNWMTLVHRGQVIDAYRMFLGLMEDVPNRKEVLAELV
                     FAGLIDVQDRMLFNRSYTTGHKAYRARSTVEIGNAIGWDDPAAHHVLYAGALDIAVGP
                     RWYSTYEMACNLVKMHIEGEALHAVPYGGVSEAEVALLRNSKEPLNQEESAGLIDVLL
                     HGQEPDNLHYVTKLLKAGKDPRRILDAMQIAASQIVLQTTEPNDFSMPHHCYEYCNTL
                     AWFFDNFDHPRRLRLIYLLASYLNRVAGHQKGINDVHSVATKPPSGADKLTAGQLLER
                     VDAAVCALNGPESLAWTQAYLDNVADRGPLVQRIALAATKLGNDPHNQEIAQCMLMDF
                     GTNRHADRDRLLLAAAYHTAMHRKYGDPLEPARRFGDAMGVALQ"
BASE COUNT          505 a          886 c          892 g          576 t
ORIGIN      
        1 gcgagaactc gacgcggctg ctgcgcatcg agggcctcaa catggacaag gtgaagcggt
       61 tcccatacga ggctgtcgat acagtcaacg ctgcggcgga atgatctgct tctcttcgat
      121 gacgtagctc ttgatctggc ctatgaacag cgcgccgtcg tccgtcagcc gcttcatttc
      181 cggcgtggcg gcggcgcgcg taagagccgc ttcatcgtcg taccaaagtt cggcaatacc
      241 gtcgacttcg acgtccgtct ccggaatatc gggtcgtgtt cgcgtgccga tgatgtggga
      301 ctgcacgtag cggcgcacgc ccggcacggc atgcgcaagc gggccgtgga tttccagcca
      361 atgcttcacg aactgctcgt gagtgagccc gggcttgcgc gtcagcaggc caacgacctt
      421 gatcatgccg cctggtggtc catcgtcgaa agctcacagg cgaccgtggt ccggtgcatg
      481 tcgcggacct cggcattatc gaacggacgc gcccggtgca tggtgacgcg attgtcccac
      541 atcacgaggt cccactgctt ccatacatgc gaatagacga acttgcgctg cgtcgcgtgt
      601 tcggtgagat cgcgcaggaa ggcgcgtgct tccggcaccg gccaaccaag gattgcgccg
      661 gcgtgtgacg ccaggaacag cgacctgcgt ccggtcaccg gatggcgacg caccaggcgc
      721 tgcctaaccg gcgcgaattt ctggcgctcc tcatcggtga agtcggaaaa gcccagcaca
      781 gcacgcgaga acagctggct atgttcgcag atcaggttct cgcattcggc ctttgtctca
      841 tcgtccagag catcgtacgc tgcgcgcatg tcggcgaatt ccgtgttgcc gccggcggac
      901 ggaatgcgcc gcgccgacag caacgaatat ttcgccgaca ccgccttgaa tgaactgtct
      961 gagtgccaca gccggttgcc cagcccgaac agccgccgcc gatcatcgcg cgcgaggatc
     1021 tcgttgttct tgttcaggtt ggaaatgtcg ttcacgtcca tcgacagacg acgttcggaa
     1081 ccttgtgcga tgtcgccggt ggcctgctcc agcgcgccga agttgcggct gaaagcgagc
     1141 tgggtctcgt cggtgaagtg ttggtcgcgg aagaccagca ctccatattg atccatgccg
     1201 gccgcgatcg ccgcagcttc cgcgtgcgac agcggttgag tgatgtctat gccagatacc
     1261 tcaccggcga agaaaggacg cgcgaccgga tcgatcgcgc ggatcgatgc ggtcatgcat
     1321 agatccgggc gcaagcccgg ccttccgtcg atatggacgg gcttgacccg gccatcactg
     1381 cagcgccact cccatcgcat caccgaagcg ccgtgccggc tctagcgggt cgccgtactt
     1441 ccggtgcatg gcggtgtgat acgccgctgc cagcagcagc cggtcgcggt cggcgtgccg
     1501 gttggtgccg aaatccatca gcatgcactg cgcgatttcc tgattgtgcg gatcgttgcc
     1561 cagcttggtt gcggccaacg cgatgcgctg caccagtgga ccacgatcag ccacgttatc
     1621 cagataggct tgcgtccacg ccaggctctc aggtccgttc agtgcacata cagccgcatc
     1681 gacacgttcg agcagttgcc cggcggtgag cttgtccgcg ccgctcggcg gcttggtcgc
     1741 caccgaatgc acgtcattga tgcccttctg atgtcctgca acgcgattca ggtaggacgc
     1801 cagcaggtaa atcagccgca agcgccgagg atgatcgaaa ttgtcgaaga accaggcgag
     1861 cgtattgcag tactcgtagc agtggtgcgg catggagaag tcgttcggct ctgtcgtctg
     1921 cagcacgatc tgcgaggcgg cgatctgcat cgcgtccagt atacggcggg gatccttgcc
     1981 ggctttcagc agtttcgtga cgtagtgaag gttgtcgggt tcctggccgt gcagcagcac
     2041 atcgatcagg ccggcgctct cctcctgatt gagcggctcc ttgctgttgc gcagcagcgc
     2101 aacctcagcc tcgctgacgc caccgtaggg tacggcgtgc agcgcctcgc cttcgatgtg
     2161 catcttcacc aagttgcagg ccatctcgta cgtggagtac caacgtggcc caacggcgat
     2221 atccagggcc ccggcgtata gcacatgatg cgctgccgga tcgtcccagc cgatggcgtt
     2281 gccgatctcg acggtggaac gggcgcgata ggccttatgg ccagtcgtgt aggagcggtt
     2341 gaacagcatt cggtcttgca cgtcgatcag gccggcgaac accaattcag ccaacacttc
     2401 cttgcggttc ggcacgtcct ccatcaggcc gaggaacatg cgatacgcgt cgatcacctg
     2461 gccgcgatgt actagcgtca tccagttgtt caggcgctcc ttcagcgggc cgtcctggcg
     2521 cagcggctcc tggtcaggcc agtacaccac cggctccggt ggcttctcca ccgtgccgag
     2581 cttgccgcgc atcctggtgt aatgacctgg cgctttgtcg atcttctggt tccagatatc
     2641 gagcccgctc gggatgtacc agattgtctg cgccatcggc agcatcgcca gatcctgcgg
     2701 caccatcttc gccaggttca ccgtggcgcg ggcgctcagc aggcagtgat cattgttgac
     2761 gaagttggga tagccgtcgt cgatacgctc gtgatatggc acatgcgtgt agggcgcgtg
     2821 gatgcgcacg ccggcgccga tgatttcgtt cagtgggcg
//
LOCUS       DATGEM010000010         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1597482, whole genome shotgun sequence.
ACCESSION   DATGEM010000010 DATGEM010000000
VERSION     DATGEM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597482"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..631
                     /locus_tag="VKQ27_00185"
     CDS             161..631
                     /locus_tag="VKQ27_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HLY87376.1"
                     /translation="MEDILASIRRILSEDETTPPAEASPPHKPDVEPSDGVLVLDPRM
                     MVPEPTRVVEETPVAEPPMQSEHPAVPVVVPPPTGLVAPEAAAAAASSVWSLVQTLAA
                     ERYSQVRSGGPTIEDLVREEIRPLLKEWLDVHLPPLVERLVRAEIERVVGRVVP"
     gene            689..3346
                     /locus_tag="VKQ27_00190"
     CDS             689..3346
                     /locus_tag="VKQ27_00190"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLY87377.1"
                     /translation="MLDKTYNPAEIETRLYEGWEQRGSFACDPDSNATPYAIMIPPPN
                     VTGSLHMGHALTFTVQDTLIRWQRMKGLDVLWQPGTDHAGIATQMVVERLLADQGVDR
                     RELGRDKFIERVWQWKAESGGTITRQLRRLGASLDWPRERFTMDDGLSRAVRKVFVDL
                     YRQGLIYRDRRLVNWDPKLQTAISDLEVENLEVKGSLWHIRYPIEGTDRFISVATTRP
                     ETMLGDTAVAVHPSDERYSDLLGKFAILPLVGRRIPIIADEYSDPEKGTGAVKITPAH
                     DFNDFAVGQRHSLPMPSILDRQARVTLAEVADVLAPDAAFVRTLEGVDRSAARKAIVA
                     ELERLGLLEKAEPYTHQVPHGDRGGVPIEPMLTTQWYANAEVLAKPAIEAVETGKTVF
                     VPKQWENTFFAWMRDIQPWCISRQLWWGHRIPAWYGPDGAVFVAFDEEEAKAEARAKY
                     GRDEELTQDEDVLDTWFSSGLWPFSTLGWPDKTKELARYYPGDVLVTGFDIIFFWVAR
                     MMMQGIHFMDDVPFRTVYIHGLVRDERGQKMSKSKGNIIDPLELIDRYGADALRFTIC
                     ALTGPGRDVKLGAARVESYRRFVTKLWNAARFCEMNGITPDASFDPSRATLPLTRWIL
                     DALNRAIAEASAALEAYRFDEYAAACYRFTWDTFCDWFVEFAKPVLAEPEVRQTAAHV
                     LGTILRLLHPAMPFVTEELWDRFGYGEPYSLIRTAWPETVAVSDAGVARAELDWVVRL
                     ISEVRSVRTEMNVPPGTLTPILLRDASAETLARGQRWVEAIRRLARASELRGLDGAMP
                     KGAAQVVLDEATVVLPLADVIDVGAERARLGKERDKTAGEARKVAQKLDNADFVRRAP
                     EEVVEENRERLAAMQADIARLEAALQRIE"
     gene            3416..4150
                     /locus_tag="VKQ27_00195"
     CDS             3416..4150
                     /locus_tag="VKQ27_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLY87378.1"
                     /translation="MIFVLICVAGVVIGTAIGATGIGGVLLVPFLTLSLGIDVKQAIG
                     AALLSYLPSCVVAVWLYGRRGSIPWREAWFLCIAALPMAWLGARAVQYAPARLLMAAI
                     GILLLAGGIYSLLPPRMGGHRRLGKPTLLMLGGVTGFLAALTGAGGAFVLLPILLLLD
                     MPVLPAIGLGMIIALPIAGLASIANLLSGTLDIWLAVMLGVTLTIGIAIGTPIAHALP
                     QRLLRRLLGSAVVIAGAAMLLRTAFS"
     gene            4167..>5098
                     /locus_tag="VKQ27_00200"
     CDS             4167..>5098
                     /locus_tag="VKQ27_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY87379.1"
                     /translation="MSSTTIRGADIVMRTLERAGHRTIFTLSGNHIMSLFDAAIETRH
                     RLVHTRHEAAAVHMADAWGRLTGEPGIAMVTGGPGHANAAAALMTAQGQESPLVLLSG
                     HTETNQLGRGGFQELRQAEVAAPMAKASWTATATETLGADVAKAIRIARSGRPGPVHL
                     SLPSDLLDATVGVDTVVWPDADAFTAAPVTLSDAAAEAILGIVAAAERPFVLAGPALA
                     NRTGRELLARLEAAMKVPTAIMESPRGFNDATLGAFAAAIRRADVIVLLGKALDFTLK
                     FGEAPAIDAGCRWVVIDPEAALVDRAIRERGALVA"
BASE COUNT          842 a         1423 c         1885 g          948 t
ORIGIN      
        1 aaactgccta ctatgaagcc gtcaagaaca agtgggctgg acttggtgac tacgccaccg
       61 accagcctaa ccgctaacgg ggcgtgccac catgagcgga gcgcaatcac cccagggtgc
      121 ggccggcgcg gccggatcga cgactgcagg cgatccgtcg atggaagata tccttgcctc
      181 gatccggcgg atcctgagcg aagatgaaac cacgccgccg gcagaagcct cgccgccgca
      241 caagccggat gttgagccga gtgatggcgt gctggtgctg gatcccagga tgatggttcc
      301 ggagccgacg cgtgtggtcg aggagacgcc agtggctgag ccgccaatgc aatccgagca
      361 tccggccgtg ccggtggtgg ttcctccgcc gactggcctg gtggcgcccg aagccgccgc
      421 cgctgcggct tcctcggttt ggagcctcgt gcagacgctg gcggcggagc gatactcaca
      481 ggttcgtagt ggcggaccca ccatcgagga tctggtgcgc gaggaaatcc ggccgttgct
      541 gaaggagtgg ctggatgtgc atctgccgcc cctggtcgag cgcttggtgc gggccgagat
      601 cgagcgcgtc gtaggccgcg tcgtaccctg agtcgtattg ccatcgtcgg ccttctgcgg
      661 catccgcgtg aggccgcagg agagcgagat gctggacaag acgtataacc ccgccgagat
      721 cgagacccgc ctgtatgaag gctgggaaca gcgcggttcg ttcgcctgcg atccggattc
      781 caatgcgacg ccctacgcga tcatgatccc gccgccgaac gtgacgggta gcctgcacat
      841 gggtcatgcg ctgacgttca ccgtgcagga cacgctgatc cgctggcagc ggatgaaggg
      901 gctcgacgtg ctgtggcagc cgggcaccga tcatgccggc atcgcgacgc agatggtggt
      961 ggagcggctg cttgcggacc aaggcgtcga tcggcgcgaa ttggggcgcg acaagttcat
     1021 cgaacgtgtg tggcaatgga aggcggagtc cggtgggacg atcacccggc agttgcgccg
     1081 gctgggggcg tcgctggatt ggccgcgcga gcggttcacg atggacgacg ggttgtcgcg
     1141 ggccgtgcga aaggtgttcg tcgatctgta ccggcagggg ctgatctatc gcgatcggcg
     1201 gctggtgaac tgggatccga agctgcagac agcaatttcg gacctcgagg tggagaatct
     1261 cgaggtgaag ggatcgctct ggcacatccg ctacccgatc gaaggcactg accgattcat
     1321 ttccgttgct acgacccggc ctgagacgat gctgggtgat acggccgttg cggtgcatcc
     1381 gtctgacgag cgttatagtg acctgctggg caagtttgcg attctgccgc tggtgggacg
     1441 gcgcattccg atcatcgcgg atgagtattc cgatccagag aagggtaccg gcgccgtgaa
     1501 gatcacgccg gcgcacgact tcaacgattt cgctgtgggg cagcggcact cgctgccgat
     1561 gccttcgatc ctcgaccgtc aggcgcgggt gacgctggcc gaggtcgctg atgtgctagc
     1621 accggatgcg gcgttcgttc gtacgctgga gggtgtggac cgcagtgcgg cgcgcaaggc
     1681 gatcgtggcg gaactggagc ggcttggcct gctcgagaag gccgagccgt atacacatca
     1741 ggtgccgcat ggcgaccggg gcggcgtgcc gatcgagccg atgctgacga cgcagtggta
     1801 cgccaatgcg gaggtgctgg cgaagccggc gatcgaggcg gtagagaccg gcaagaccgt
     1861 gttcgtgccg aagcaatggg agaacacgtt cttcgcctgg atgcgcgaca tccagccgtg
     1921 gtgtatctcg cgccagctct ggtgggggca tcggatccca gcatggtacg ggccggatgg
     1981 tgcggtgttc gtggcatttg atgaggagga ggcgaaggct gaggcgcggg cgaagtatgg
     2041 tcgcgacgag gagctgacac aggacgagga tgtgctggat acctggttca gctctggcct
     2101 gtggccgttc tcgacgctgg gctggccgga caagacgaag gaactggcgc gatactaccc
     2161 gggcgacgtc ctggtgaccg gattcgacat catcttcttc tgggtcgcgc ggatgatgat
     2221 gcagggcatc cacttcatgg acgacgtgcc gttccgcacc gtgtatatac atggcctggt
     2281 gcgcgacgag cgcggacaga agatgtcgaa gtcgaagggc aacatcatcg atccgctgga
     2341 gctgatcgat cggtatgggg cagatgcgct gcgcttcacc atctgcgcgc tgaccgggcc
     2401 agggcgggat gtgaagctgg gggccgctcg cgtggagagc tatcgccggt tcgtcaccaa
     2461 gctgtggaac gcggcgcggt tctgcgagat gaacgggatc actccggatg catcgttcga
     2521 tccgtcacgg gcgacgttgc cgctcacgcg gtggatcctg gatgcgctga accgggcgat
     2581 tgcggaggcg agcgcggcgc ttgaagcgta ccgattcgat gagtatgccg cggcctgtta
     2641 ccgcttcacc tgggacacgt tctgcgactg gttcgtggaa ttcgccaagc ctgtactggc
     2701 ggaacccgaa gtgcggcaga cggcggcgca tgtcctgggt acgatattgc gcctgctgca
     2761 tccggcgatg ccattcgtca ccgaggaatt gtgggaccgg ttcgggtacg gcgagccata
     2821 tagcctgatc cgaacggcgt ggccggaaac cgtggcggtg tcggatgcag gagtcgcgcg
     2881 cgcggagctg gattgggtgg tgcggctgat ctccgaggtg cgctcggtgc ggaccgagat
     2941 gaatgtgcca ccggggacgc tgacgccgat cctgctgcgg gatgcatcgg cagagacact
     3001 ggcacgcggc cagcggtggg tggaggcgat ccgtcggttg gctcgcgcgt ccgagttgcg
     3061 cgggctcgat ggcgccatgc cgaaaggagc ggcgcaggtg gtgctggatg aggcgaccgt
     3121 cgtgctgcct ctggcggatg tgatcgacgt gggcgcagaa cgtgcccgcc tgggcaagga
     3181 acgcgacaag actgccggcg aggcgcgcaa ggtcgcgcaa aagctggaca acgcggattt
     3241 cgtgcgtcgc gcgccggaag aagtggtgga ggagaaccgc gagcgtctcg ctgcgatgca
     3301 ggcggatatc gcacggctgg aagcggcgct gcagcggatt gagtagattg acggtgagcc
     3361 ggcgaggccg tcttgccctt ctcccttacc gcaaggagaa gggtagttta gcgggatgat
     3421 ctttgtcctg atctgcgttg cgggcgttgt catcgggacg gcgatcggcg ccaccggcat
     3481 tggaggagtg ctgctggtgc catttcttac gctgagcctc ggcatagatg tgaagcaggc
     3541 gatcggggcc gcgctgctga gctatctgcc gagctgcgtc gtcgctgttt ggctctatgg
     3601 caggcgtggt tcgattccat ggcgcgaggc gtggttcctt tgcatcgcgg cattgccgat
     3661 ggcttggcta ggggcgcggg cggtgcagta tgcgccggct cggttgctga tggccgccat
     3721 tggaattttg ttgctggcag gcggaatcta ttcattgctg ccaccgcgca tgggtggtca
     3781 ccgtaggttg ggcaagccaa cccttctgat gcttggcggg gtcaccgggt tcctggcggc
     3841 gctgaccggc gccggcggag ccttcgtgct gttgccgatc ctgctgctgc tcgacatgcc
     3901 ggtgctgcca gcgatcgggc tggggatgat catcgcattg cccatcgcgg ggctggccag
     3961 catcgcgaat ctgctgtcgg gaactctgga tatctggctc gcggtgatgc ttggcgtgac
     4021 cctgaccatc gggatcgcga ttggcacgcc gatcgcgcac gccttgccac agcgtcttct
     4081 gcgtcgtttg cttggttcgg ctgtggtgat cgccggagcc gcgatgcttc tgcgcaccgc
     4141 cttttcgtga acggaaacca aaacacatga gcagcactac catccgcggc gccgacatcg
     4201 tgatgcgcac gctggaacgc gcaggccacc gcacgatctt cacgctgtcc ggcaaccaca
     4261 tcatgtcgct gttcgatgcg gcgatcgaga cgcggcatcg gctggtgcat acgcgacatg
     4321 aggctgcggc ggtgcatatg gcggacgcct gggggcggct gaccggcgag cctggcattg
     4381 cgatggtcac cggtggtccg gggcatgcca atgccgcggc ggcgttgatg acggcgcagg
     4441 ggcaggaatc gccactggtg ctgctgtcgg ggcatacgga aaccaatcag cttgggcgcg
     4501 gcgggttcca ggaactgcga caggcggagg tggcggcgcc gatggcgaag gcgtcgtgga
     4561 ccgcgaccgc gaccgagacg ctgggagctg atgtggcgaa ggcgatccgg attgcacggt
     4621 cggggcggcc ggggcccgtg cacctgagct tgccgtccga tctgctggat gcgacggttg
     4681 gtgtggatac ggttgtgtgg ccagatgctg atgcattcac ggcggcgcca gtaacgctga
     4741 gcgatgcggc ggcggaggcg attctcggga ttgtcgcagc ggccgagcgg cctttcgtgc
     4801 tggcggggcc ggcattggcg aaccgcactg ggcgagagct gctggcgcgc cttgaggcgg
     4861 cgatgaaggt tccgaccgcg atcatggaaa gtccgcgcgg tttcaatgat gccacgctgg
     4921 gagcgttcgc cgctgcgatc cggcgcgccg atgtgatcgt gctgttgggg aaggcgctgg
     4981 attttacgtt gaagttcggc gaggcgccgg cgatcgatgc tggttgcaga tgggtcgtga
     5041 tcgacccgga ggcggcgttg gtagatcgcg ctatacgcga gcgcggcgcg ctggtcgc
//
LOCUS       DATGEM010000011         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1025146, whole genome shotgun sequence.
ACCESSION   DATGEM010000011 DATGEM010000000
VERSION     DATGEM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025146"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VKQ27_00205"
     CDS             complement(<1..332)
                     /locus_tag="VKQ27_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY87380.1"
                     /translation="MRLLRLTLTDFRSYAALTWRPAARIAVLFGPNGSGKTNLLEAVS
                     LLAPGRGLRNARNVDLPRHGGAGGWAVAGRFATADGEVDIGTGSAPESLRSGPGTDPS
                     HRRVFRLDG"
     gene            857..>2186
                     /locus_tag="VKQ27_00210"
     CDS             857..>2186
                     /locus_tag="VKQ27_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLY87381.1"
                     /translation="MGDTLLQGSILADRYLHTSYRASATELTAWLARFGDQPEATGIR
                     DLLERLAPGAAISTIAAAGFAGRVASTARKGVRPAQVRALFVRNHDDQVIRETSALLA
                     GAEPANAESLFIGGLASWRQEQNAAATAFFDAAYRAATSSSLRAASAFWAGHVQQRLG
                     NRGGFAVWMRRAALEGDAFYAMVARRALGPSPACAAGGAIGNADVEALVATPQGKRAF
                     ALVQIGEKRLAEAELRALWVDTAQDGVFDRSLALTAKALGFGQFAAEIERSSAARPDT
                     GMPTRLQPAGGFIVDPPLVYALVRHESNFHPAAVSAYGARGLMQIMPRTAHAVAGRAA
                     ERLQDPAVNLSIGQQYLLTLADDDAIDGDLVRLLAGYGQGQGGLRKWVDDVRDNGDPL
                     MFIEAIPSGSTRAFVRDALVYSWRYAAAMAIPATSLDALASGQYPRLIRAT"
BASE COUNT          361 a          669 c          752 g          404 t
ORIGIN      
        1 ccatcaaggc ggaacacccg tctgtggctg gggtcagtcc cgggcccgga cctaaggctc
       61 tcaggcgcac tgccggtacc gatatcgact tcgccatcgg cagtggcgaa gcgtccggcg
      121 acggcccaac cgccggcacc gccgtgacgg ggcagatcaa cattccgcgc gttacggagg
      181 cctcggccag gtgccagcag ggagactgct tcgagcaggt tggttttgcc gctgccattc
      241 gggccgaaca gcaccgcgat gcgtgccgcc gggcgccatg tcagggcggc ataactacgg
      301 aaatccgtca gcgtcaggcg gagcaggcgc atggccggat gtgcgacgac ggcaccccaa
      361 aggtcaattg ccgcagtgtg cgaataccac ggcggttaac ggtccaggcc gaaaagttct
      421 tagcaatata gtattcgcaa ataatactat tacgtcgcat cataattgat ccaactggtt
      481 gccaacgcat gtagatacgc aggcggtttg ccatgattcc gtcagatttc caggtagtgt
      541 tgccaaccag gacttgggtg tgtattggac gagcatgcaa gggcgagggg cacttcggac
      601 ccgtgctctg gcggtttggg cggggatggt cggtgttgtt cttgcaacat cggtgcacta
      661 ttcttttgcc caaacgccgg gaacgggcga aacgggcgac cagacagcat tcgcggtgcc
      721 acggctgggt atgcacggcg cggcaggcgt tggaatccca caaccgcttc cgccgagcga
      781 ggctgctctc gcgcggaaga ttttctctct ccaagattcg ggttcgctgg cggaagcggc
      841 tcgtgaatac tcgcgattgg gggataccct tctacaagga tcgatcctgg cggatcgcta
      901 cctgcacact tcatatcgtg cctcggcgac ggagctgacg gcctggctcg cccgtttcgg
      961 cgaccagccc gaggcgaccg gcatccggga tctgctggaa cggcttgcgc ctggtgccgc
     1021 gatttccacg atagcggcgg ccgggtttgc cggccgggtg gcttcgactg cccgcaaggg
     1081 cgtgcggccc gcccaagtgc gcgccctgtt cgtgcggaac catgacgacc aggttatccg
     1141 ggagacttcc gcgctgcttg ctggcgcgga accggccaat gccgagtcac tgttcatcgg
     1201 cggactggct tcctggcggc aggaacagaa cgccgccgcc acggcctttt ttgatgctgc
     1261 ctatcgtgcc gccacatcgt cgtccctgcg cgcggccagc gccttctggg cgggccatgt
     1321 gcaacagcgc cttggcaacc gcggcggctt tgccgtctgg atgcgccgcg ccgcgcttga
     1381 gggtgatgcg ttctacgcca tggttgcccg ccgcgctctg gggccatctc cggcctgcgc
     1441 ggccgggggc gcgatcggca atgcggacgt tgaggctctg gtggcgacgc cgcaagggaa
     1501 acgggcgttc gccctcgttc agattggcga gaagcgtctg gccgaggcgg aattacgtgc
     1561 gctctgggtc gatacggcac aggacggcgt gttcgaccgg tcgctggcgc tgaccgccaa
     1621 ggcgctgggc tttggccagt ttgccgcaga gatcgagcgc agcagtgcag ccaggcccga
     1681 caccgggatg cccacgcggc tgcagccggc tggcggcttc attgtcgatc cgccgctggt
     1741 ttatgcgctg gtaaggcatg aatcgaattt ccatcccgcg gcggtgtcgg cgtatggcgc
     1801 gcggggcctg atgcagatca tgccgcgtac tgcccatgcg gtcgccggcc gggcagcgga
     1861 gcggctccag gacccggcgg taaacctgtc gatcgggcag caatacctgc tgacgctggc
     1921 ggatgacgac gcgatcgacg gcgacctggt ccgcttgctt gccggctatg gccaggggca
     1981 gggggggctg cggaaatggg tcgatgacgt tcgagacaac ggcgatccgc tgatgttcat
     2041 cgaggcgatc ccaagcggca gtacccgagc attcgtgcga gatgcgctgg tgtattcgtg
     2101 gcgctatgcc gcggcgatgg cgattccggc gaccagcctg gacgccttgg cgagcggcca
     2161 gtatccgcga ttgatccggg ccaccg
//
LOCUS       DATGEM010000012         8877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_111994, whole genome shotgun sequence.
ACCESSION   DATGEM010000012 DATGEM010000000
VERSION     DATGEM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111994"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VKQ27_00215"
     CDS             complement(<1..891)
                     /locus_tag="VKQ27_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87382.1"
                     /translation="MPAAPVRRSGAVRDTSIRTYRAKRDFTKTHEPAPSKRSAKNGSM
                     FVVQKHQAHRAGLHWDFRLEHDGVLWSWAVRKGPSLDPADKRIAVHVEDHPLDYANFE
                     GSIADGQYGAGHVELWDRGTWQPLIDPDAGMRDGEIKFVLDGQRLHGRFTLVRLKPRP
                     GERGRQDNWLLIKGHDDDERKGADAPTIEQKAPAPGARDEAPAEGARRARLPKHVTPE
                     LASVVEEPPDGKGWISEIKFDGYRLLCWKDGSEARIMTRNGLDWTDRLPAVARTVARL
                     NAHSAIVDGELVSLDKSGVSS"
     gene            complement(897..1403)
                     /locus_tag="VKQ27_00220"
     CDS             complement(897..1403)
                     /locus_tag="VKQ27_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HLY87383.1"
                     /translation="MRKLTTSCLAAGLIGLVAGQASAELSSADRNFATKAASGGLAEI
                     QTAQMAKQRAESPQVKAFADRMISDHTQANNDLQQIAQQENLTLPSEPSKQERSAAQR
                     VGGMTGAAFDRTYAQQEVRDHQQHIALFQQEANSGRDPALKQFAQKTLPTLRQHLQMA
                     QALTTDQQ"
     gene            complement(1425..3626)
                     /locus_tag="VKQ27_00225"
     CDS             complement(1425..3626)
                     /locus_tag="VKQ27_00225"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY87384.1"
                     /translation="MGIIGQPIDRVDGRLKVTGRAKYAAEFAVPGVVHAVLVQSTIGA
                     GSITGFDLSEAQSTPGVLAIITPQNAPKLTMKGGTQQTVRAPLLQDMAIYYNGQHVAV
                     VVADTLERASDAAARVKVRYQAAEPVTSMDAVLDQAYMPKNFRNGERPADSQRGNPDL
                     SFSAGAANVDATYITPMEHHNPMEPHATIAQWDADRLTVWTATQGISGTQTTLASMFG
                     LDPSNVRVICPYVGGGFGCKGSTWPPVTLAAMAAKVVGKPVKLVLTRAQMYTSNGYRP
                     RTIQKLRFAADDQGHLVSMRHDGFSQMSQAPLGEFAEPVGLATEMLYDCPNVAVTHRL
                     VATNAGLPTYMRAPGLASGNFALESAIDELAVASRMDPLEFRLRNYAETDEHLDKPYA
                     SKALRACYQQGAELFGWSRRSHEPRSMRDGNMLTGWGMATSTYPTHRLPASARVRLGA
                     DGTALVQVGTQDIGTGTYTVMSQVAADTLGMDVRRIQFELGDSAYPHAPVSGGSMTVA
                     SVGPAVLAACQAMRDKLFDMALDDQRLSWSGTDRASLQLRGEAVIGPKGRIPVAELLS
                     HQRQDFVETSSNVKPGEEEKRYAMHAFGAQFIEVRVDPDLGEIRVSRIVAAFDGGRIL
                     NAKTAHSQLIGGIVFGLGMALLEETHVDGETGRVVNANVAEYLLPVNADVPDITAILV
                     KNDELVSNPLGAKGIGELPMVGVAAAVANAVYHATGVRVRKLPIRIEDLLV"
     gene            complement(3640..4617)
                     /locus_tag="VKQ27_00230"
     CDS             complement(3640..4617)
                     /locus_tag="VKQ27_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HLY87385.1"
                     /translation="MQAFSLHRPRSAADAIAAAQSQGSKYIAGGSDLMQLMKDNIEVP
                     TRLVDLEHVGLTGIHSDSKGLRLEAMARMSDVASHPAVRERWPVISEALLASASPQVR
                     NMGTIGGNLLQRTRCGYFRDSGFNCNKREPGSGCPAIKGESRMLGVLGVSYNCIAPHA
                     SDLAVALMALDAALELRGASGARRQVPLAEFYRLPGDTPHIETVLAPGEMIEAVIVAE
                     SPAARNSHYLKVRDRTSFEFALVSAAVVLDTDGGKIRDIRVAAGGVGTRPWRLPEVEA
                     ALHGATLTDGALREASARAGVGARPTEQNQFKLTLLRRTVLRALQTASA"
     gene            complement(4621..5082)
                     /locus_tag="VKQ27_00235"
     CDS             complement(4621..5082)
                     /locus_tag="VKQ27_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY87386.1"
                     /translation="MEHVELQVNGQRLPLTLDPRTSLLDALREHAGLTGTKKGCDRGA
                     CGACTVHVDGRRVVSCLTLAARCEGKAVTTIEGLSAHGELHPVQAAFLHHDGFQCGYC
                     TPGQIMSAAALIREGHTKSDGEIREWMSGNICRCGAYPNIVTAIRDAAKEG"
     gene            complement(5298..6086)
                     /locus_tag="VKQ27_00240"
     CDS             complement(5298..6086)
                     /locus_tag="VKQ27_00240"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY87387.1"
                     /translation="MSVPTVRNVAREKLEQGQLSLGCGVRLTRSVEIGVAMKAAGFDW
                     LFLDLEHGTMSIDAAAQIATAALDAGIAPLARVPNGEYSIATRLLDNGVLGIVMPHVD
                     SADEAREVVEKLKYPPIGHRSMGGTGPHYNLRSASTGDAAAALNAANLTVVMLETPKA
                     IENAEAIAAVPGVDVLLIGTNDLCAELGIHGDFGNDRVAEAYRIMIAAAHKHGKFPGM
                     AGIYNEPLMQRYIAMGARFILSGNDANFLVAAAGQRTAFLRKTL"
     gene            complement(6083..6976)
                     /locus_tag="VKQ27_00245"
     CDS             complement(6083..6976)
                     /locus_tag="VKQ27_00245"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY87388.1"
                     /translation="MLTKSQIDEYNEVGAIVVPDILTGNEVSRLRQVTSEFVDRARGV
                     TAHNEIYDLEDTHTPENPRVRRIKTAHLHHPEYARLMRHPKIVAVLQDLWGPDIRFDT
                     AKLNLKSAGFGAPVEWHQDWAFYPHTNDDLAAVGVMFDDMEMANGPLMIIPGSHRGPI
                     FDHHVEGVFVGAMSPDNQEVDFSSAIPLTGKAGSITVHHVRAVHGSAPNISDMDRRLL
                     LFQFRCADAWPLLGFRDGIEKYKELMVCGQHREPRLADAPVRMPLPPAKLQGSLYENQ
                     KGMKSRFFTVPNAAREPEPVK"
     gene            complement(7024..7488)
                     /gene="mscL"
                     /locus_tag="VKQ27_00250"
     CDS             complement(7024..7488)
                     /gene="mscL"
                     /locus_tag="VKQ27_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HLY87389.1"
                     /translation="MALPTNLGKLKPSWVDEFRAFIMRGSVVDLAVGIIIGAAFTGVV
                     NSLVKDVFNPVLGLATGGIDFSNVFWALNGQHYTTLADAQHAGAPTLNLGLFINTIIN
                     FVIVAFAIFWVVKALTRMHLREDAATGPKAEAILLEIRDILKDQSAQRPAAG"
     gene            complement(7571..8191)
                     /locus_tag="VKQ27_00255"
     CDS             complement(7571..8191)
                     /locus_tag="VKQ27_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLY87390.1"
                     /translation="MSHDLFEIMRSTRSMRRLKPDPIPDDVLRQVLEAGTTAANGGNM
                     QTWRFLVIRDPATKAAAGAWYRKSWQEIVGPRYRSAAPAPGQSAEQFARMLDAAQYLA
                     DHIHEAPVWIVPCAPGGRNAGSSIFPAVQNILLACRALGLGCTLTTLHLNFEKDVEAA
                     LGLPDDVHSYAILPIGYPMGKFGPVRRSPLEHVVYQDRWGEDYRSA"
     gene            8388..>8877
                     /locus_tag="VKQ27_00260"
     CDS             8388..>8877
                     /locus_tag="VKQ27_00260"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87391.1"
                     /translation="MSITVTPTGAALGAEIGGLDLRSITDADFAQVYRAWLDHSVLLI
                     RGQVLTDDDLIAFSLRFGQLDFAPIQENGRRFVDGHPEIYVVSNVIENGVAIGSLGAG
                     EAVWHTDMSYLEDPPKASMLYAIEVPPSGGNTGFTSMYRAYELLPDELKRRLVGLRVK
                     HDG"
BASE COUNT         1530 a         2945 c         2721 g         1681 t
ORIGIN      
        1 gctcgagacg ccactcttgt cgagcgacac caactcgcca tcgacgatcg ctgaatgggc
       61 attcaggcgg gcgaccgtgc gggccacggc gggcaggcga tcggtccagt caaggccatt
      121 gcgcgtcatg atgcgcgctt cgctgccgtc cttccagcaa agcaggcgat agccatcgaa
      181 cttgatctcg ctgatccagc ctttgccgtc cggcggttcc tccaccacgg aagccagttc
      241 gggcgttacg tgcttcggca gcctggcgcg cctcgcccct tccgccggcg cttcatcgcg
      301 cgctccaggt gccggcgctt tctgctcgat cgtcggcgca tcggcacctt tgcgctcgtc
      361 gtcgtcgtgc cctttgatca gcaaccagtt gtcctgccgg ccgcgctctc ctggccgagg
      421 cttcagccgg accagcgtga acctgccatg cagccgctgc ccatccagca cgaacttgat
      481 ctcaccatcg cgcatgcccg catcggggtc gatcaatggc tgccatgtgc cgcggtccca
      541 cagttccaca tggccggcgc cgtattgccc gtcggcgatg gacccctcga agttggcgta
      601 gtcgagcgga tggtcctcga catgcacggc gattcgtttg tcagccggat ccagtgacgg
      661 ccccttccgg accgcccagc tccacaacac gccgtcatgc tccagccgaa aatcccagtg
      721 caggccagcc cgatgtgcct ggtgcttctg caccacgaac atcgacccgt tcttcgccga
      781 ccgcttcgag ggcgccggct catgcgtctt ggtgaagtcg cgcttcgcgc gataggtgcg
      841 gatcgaagta tcgcgtacgg cgccggatcg ccgcaccggc gccgctggca tgctgattac
      901 tgctgatctg tcgtcagagc ctgcgccatc tgcaggtgct gacgtaaggt tggcagcgtc
      961 ttctgtgcga actgcttcag cgcaggatca cgcccggaat tcgcctcctg ctggaacagg
     1021 gcgatgtgct gctggtgatc gcgcacctct tgctgggcat aggtgcgatc gaacgccgcg
     1081 cccgtcatac cgccgacgcg ctgtgccgcc gaacgctcct gcttactcgg ctctgacggc
     1141 aatgtcagat tctcctgctg cgcaatctgc tgcaggtcgt tgttagcctg cgtgtggtcg
     1201 ctgatcatcc gatcggcgaa cgcctttacc tggggcgatt ccgcccgctg cttcgccatt
     1261 tgcgctgttt ggatttccgc aaggccacca gacgcggcct tcgtggcgaa gttgcgatca
     1321 gcgctggata gctcggcgct cgcctgtccg gcgaccagac cgatcaggcc ggcggcgagg
     1381 caactcgtcg tcagcttgcg catgatgtgt ctccctaaac cagcttagac caataagtct
     1441 tcaatccgta tcggcagctt acgcacccgc acaccggtgg cgtgatagac cgcattggcg
     1501 acagccgccg cgacacccac catgggcagc tcgccaatcc ccttggcacc gagtgggttc
     1561 gaaacgagct cgtcattctt caccaggatg gccgtgatgt cgggcacgtc agcattcacc
     1621 ggcagcaggt attcggcgac attcgcgttg accacacggc cggtttcgcc atcaacgtgc
     1681 gtttcctcca atagtgccat gccgagcccg aacacaatgc caccaatcaa ctgactgtgc
     1741 gcggtcttgg cattcagtat acggccgccg tcgaaagcgg ccacgatgcg actcacgcga
     1801 atctcgccga gatcggggtc gacccgcacc tcgatgaact gcgcgccaaa cgcatgcatg
     1861 gcgtaacgct tctcttcctc gccgggtttc acgttgctgc tggtctcgac gaaatcctgt
     1921 cgctggtgtg acagcagctc cgccaccggg atgcggccct tcggcccgat caccgcttcg
     1981 cctcgcagct gcagcgatgc gcgatcggtg ccgctccagc tcagccgctg gtcatccaat
     2041 gccatgtcga acagcttgtc cctcatcgcc tggcacgccg ccagcacggc cggcccgacg
     2101 cttgccaccg tcatcgagcc accggacaca ggggcgtgcg ggtaggcact gtcaccgagc
     2161 tcgaactgga tgcggcgcac gtccataccc agcgtatcgg cagcgacctg cgacatcacg
     2221 gtataagtgc cggtgccaat atcctgggtg ccgacttgca ccagggccgt gccgtcggcg
     2281 ccaagccgca cccgtgcgga cgccggcagc cgatgcgtgg ggtaggtgct ggtcgccatg
     2341 ccccaaccgg tcagcatgtt gccgtcgcgc atggaccgag gctcgtgcga acgccgcgac
     2401 cagccgaaca gttccgcccc ctgctggtag caggcacgca atgccttgct ggcatagggt
     2461 ttgtccagat gctcatcggt ctcggcgtag ttgcgcagcc ggaactccag cggatccata
     2521 cgcgacgcaa ccgccagctc atcgatcgcc gactccaacg cgaagttgcc gctggcgagt
     2581 cctggcgcgc gcatatacgt cggcagcccc gcgttggtcg ccaccagccg atgcgtcacc
     2641 gcaacgttgg ggcagtcgta cagcatctcg gttgccagtc cgaccggctc ggcaaattcg
     2701 ccaagcggtg cctgcgacat ctgcgagaac ccgtcatgcc gcatcgacac cagatgccct
     2761 tgatcgtcgg cggcaaagcg cagcttctgg attgtgcgcg gccggtatcc gttcgaggtg
     2821 tacatctgcg cccgcgtcag aaccagcttc accggcttac cgaccacctt cgccgccatc
     2881 gcggccaacg ttaccggcgg ccaggtgctg cccttgcagc caaagccacc gccaacatag
     2941 gggcagatta cccgcacgtt cgacggatcc agaccgaaca tggaggcaag tgttgtctgc
     3001 gtgccggaga tcccttgcgt tgccgtccat acggtgagcc gatcggcgtc ccactgcgcg
     3061 atggtcgcgt gcggctccat cggattgtgg tgctccatcg gcgttatgta cgtggcatcc
     3121 acattagcgg cgccagcgga aaaactgagg tcggggttgc cacgttgtga atcggccggc
     3181 cgctcgccgt tgcggaagtt tttcggcata taggcctgat cgagcacggc atccatcgac
     3241 gtgaccggct ccgcggcctg gtaccgcacc ttgacacgtg ccgccgcatc gctggcgcgt
     3301 tcaagcgtat ccgccaccac gaccgcaacg tgctggccgt tgtagtagat ggccatgtct
     3361 tgcagcagcg gcgcgcgcac ggtttgctgc gtgccgccct tcattgtcag cttcggcgcg
     3421 ttctgcggcg tgatgatcgc caatacgcct ggcgtcgatt gcgcctcgct gagatcgaag
     3481 cctgtgatcg agccggcgcc aatcgtactc tgcaccagca cagcatgcac gacaccaggt
     3541 accgcgaact cggcggcata tttcgcgcgg cctgtcacct tcaggcgccc gtccacacga
     3601 tcgattggtt gtccgatgat tcccatcaca cgcctcccgt caggcggacg ccgtttgcaa
     3661 cgcgcgcaga actgtgcgcc gcagcagggt gagtttgaac tggttctgct cggtcggacg
     3721 cgctccgacg ccagctcgcg ccgacgcctc acgcaacgcg ccatcagtga gcgtcgcgcc
     3781 gtgcaacgca gcctctactt ccggcagtcg ccatggccgc gtgccgacgc caccagccgc
     3841 gacgcgaatg tcgcggatct tgccgccgtc ggtatccagc actacggcgg cagagaccag
     3901 agcaaactca aagctcgtac gatcacgtac cttcaggtag tgcgaattgc gcgcggcggg
     3961 gctttccgcg acgatcaccg cctcgatcat ctcgcccggt gccagcacgg tttcgatgtg
     4021 cggtgtatcg ccaggcaggc gatagaactc cgccaggggc acctgccgcc gcgcgccact
     4081 cgcaccacgc agttcgagcg ctgcatccag tgccatgagc gctaccgcaa ggtcagacgc
     4141 atgaggcgcg atgcaattgt aactgacacc gagaaccccc agcatgcggc tctcgccttt
     4201 gatcgccgga cacccgcttc caggctctcg cttgttgcaa ttgaaaccgg agtcccggaa
     4261 gtaaccgcag cgcgtacgtt gcagcaggtt gccgccgatg gtccccatat tgcgtacctg
     4321 cggcgatgcc gaggcaagca acgcttcaga gatcaccggc cagcgctcgc gcaccgcggg
     4381 atgcgacgca acgtcgctca tccgtgccat cgcttcgagc cgcaggccct tgctgtcgct
     4441 gtggatgccg gtcagcccga catgttccag atcgaccagc ctcgtcggca cctcgatgtt
     4501 gtccttcatc aactgcataa ggtcgctgcc gccggcgatg tacttgctgc cctgtgattg
     4561 tgccgcggcg atggcatcag ccgccgaacg cggccgatga agcgaaaagg cctgcatggc
     4621 tcagccctcc ttcgcggcgt cacggatcgc cgtgacgatg ttgggatagg ccccgcagcg
     4681 acagatgttg ccgctcatcc actcgcggat ttctccatcc gattttgtgt gcccctcgcg
     4741 gatcagggcc gcggccgaca tgatttggcc tggtgtgcag tagccacact ggaatccgtc
     4801 gtgatgaagg aaggcagcct gcaccgggtg cagctcacca tgagccgaca ggccttcgat
     4861 cgtcgtgacc gccttgccct cgcagcgcgc cgccagcgtc aggcaagaca ccacacggcg
     4921 accatcgaca tgcaccgtgc aggcgccgca cgcgccgcga tcgcagccct tcttcgtgcc
     4981 agtcaggccg gcatgttcgc gcagcgcatc gagcagcgac gtgcgtgggt cgagcgtcag
     5041 cggcaggcgc tggccgttca cctgcagctc gacgtgctcc atctgatcgg gggtcgcgcg
     5101 tgccgtcggt gatggtgcgg caacgtccgc acgactgtgg tgcgaacgaa gcgccagacc
     5161 acccgccgtg ccggccaaca gcaaacggcg ggtcaaattt ggttcatcca tgtccccaat
     5221 tccccggctg actcgccggg ttacgtgggg agcgttgctt tacccgcggc tcacatcggc
     5281 gtgcgagcgc ggccgcgtta caatgtctta cgcaggaaag ccgtccgttg cccggccgcg
     5341 gcgaccagga agttcgcatc attgccggaa aggatgaagc gcgcgcccat cgcgatgtaa
     5401 cgctgcatca gcggctcgtt gtagatgcca gccatccctg ggaatttgcc atgcttgtga
     5461 gccgcggcga tcatgatccg gtaagcttct gccacgcgat cgttgccgaa atcgccgtgg
     5521 atccccagct cggcgcacag gtcgttggtg ccgatcagga gcacatcaac cccaggaacc
     5581 gctgcgatcg cctcggcgtt ctcgatcgcc ttcggtgttt ccagcatcac cactgtgaga
     5641 ttggcggcat tcagcgctgc cgcagcatcg ccggtgctgg cgctgcgcag gttgtagtgc
     5701 ggcccagtgc cacccatcga gcgatgcccg atcggtggat atttcagttt ctccaccact
     5761 tcgcgcgctt catcggcact atcgacatgt ggcatgacga tgccgagcac gccgttatcc
     5821 agcagccgcg tcgcgattga gtattcgccg ttcggcacgc gcgccagcgg cgcgatgccg
     5881 gcatcgagtg ccgccgtggc aatttgcgcc gccgcgtcga tcgacatcgt gccatgctcg
     5941 aggtcgagga acagccagtc gaagccggcc gccttcatcg cgacgccgat ctcgacgctg
     6001 cgcgtcaggc gcacgccaca gccaagcgaa agctgcccct gttccagctt ctcgcgcgcg
     6061 acattccgta cggtggggac gctcatttca caggctccgg ttcgcgtgcc gcattgggca
     6121 cggtgaagaa gcggctcttc atgcctttct ggttttcgta gagcgaccct tgcagtttcg
     6181 ccggcggcag cggcatgcgc acgggcgcgt cagcgagccg cggctcgcga tgctgcccgc
     6241 acaccatcag ctccttgtac ttctctatgc cgtcacggaa ccccagcagc ggccacgcat
     6301 cggcgcaacg gaactggaac agcagcaggc ggcgatccat atcggagatg tttggcgccg
     6361 aaccgtgcac cgcgcgcaca tggtgcacgg tgatcgaccc cgcctttccg gtcagcggaa
     6421 tggcggacga aaaatccact tcctggttgt cggggctcat cgcaccgacg aacacgcctt
     6481 ccacgtgatg atcgaagatt ggcccgcgat ggctgcccgg gatgatcatc agcgggccgt
     6541 tcgccatctc catgtcatcg aacatcacgc cgacggcggc gagatcgtcg ttggtgtgcg
     6601 ggtagaatgc ccaatcctga tgccattcca ccggcgcgcc aaaccccgcg gacttcaggt
     6661 tcagtttggc ggtatcaaag cggatgtcgg gcccccaaag gtcctgcagc accgcgacga
     6721 tcttcgggtg ccgcatcagc cgggcatatt ccggatgatg cagatgcgcc gtcttgatcc
     6781 gccgcacgcg cggattctcc ggcgtgtggg tgtcttccag gtcgtaaatc tcgttatgtg
     6841 ctgtcacgcc gcgcgcccga tcgacgaact cgctggtgac ctgccggagc cgtgacactt
     6901 cattgccggt caggatgtcc ggcacgacga ttgccccgac ttcgttgtat tcgtcgatct
     6961 ggctcttggt tagcatagcg tcctccgtgc cggcattata gcgcgaggct gcaaaaccgc
     7021 taatcagccc gcggcagggc gctgtgcgga ctggtccttc aggatgtcgc ggatctccag
     7081 caggatggcc tcggctttcg gaccggtcgc tgcgtcttcg cgcagatgca tccgggtcag
     7141 cgccttcacc acccagaaga tggcgaacgc gacgatgacg aagttgatga tagtgttgat
     7201 gaacaggccg agattcagcg ttggcgcccc ggcatgctgc gcgtcagcca gcgtggtgta
     7261 gtgttgcccg ttcagcgccc agaagacgtt ggagaagtcg atcccgccgg tggcaagccc
     7321 aagcaccggg ttgaacacat ccttcaccag gctgttcacc acaccggtga acgctgcgcc
     7381 gatgataatg ccgacggcaa gatcaaccac gctgccgcgc atgatgaagg cacggaactc
     7441 gtcgacccag gagggcttga gcttgccgag gttggttggg agagccatgc gggtttcctc
     7501 ttcctatccg ttgcaattgg tgaattcagg ggtgcgaagt ggtctaggtc aatgtcggga
     7561 tgtcagagtg tcacgccgat cggtagtcct caccccagcg atcctgatac acgacatgct
     7621 ccaaaggtga tcgccgcacc ggcccgaact tccccatcgg atagccgata gggaggattg
     7681 cataggaatg cacgtcgtca ggcagtccga gcgcagcttc cacatccttc tcgaagttca
     7741 ggtgcagcgt ggtcaacgtg cagcccaggc caagcgcccg acacgccagc agaatgttct
     7801 gcaccgccgg gaagatggac gagcctgcat tcctcccccc cggcgcgcac gggacgatcc
     7861 acaccggcgc ctcatggatg tgatcggcca gatactgcgc cgcgtccagc atgcgggcga
     7921 actgctcggc cgattgccca ggcgccggtg ccgccgaacg atagcggggt ccgacaatct
     7981 cctgccacga cttgcgatac catgcgccgg ccgcggcttt ggtcgccgga tcgcggatca
     8041 ccaggaagcg ccatgtctgc atgttgccgc cattggcggc cgtggttccg gcctccagca
     8101 cctggcgcag cacatcgtca gggatcggat ccggcttcag cctgcgcatg gagcgggtcg
     8161 agcgcatgat ctcgaacagg tcgtgtgaca tgggaaagtg ctcctggttg gctacggcgg
     8221 cggtctgccc agatatcatc cgttgcattt gcggcagacg ctcggttgca cgccgaacac
     8281 ctgcgcgaac atgctgcgga acgcagccgt gcttgtatag ccgccacatt tgccgggtgc
     8341 cgccggagac ggtatcgatg atgcaccaca cggccaggga gggtcgcatg tccattaccg
     8401 tgacgccgac aggtgccgcg ctcggcgccg agattggcgg gctcgatctg cgtagcatca
     8461 ccgatgcgga tttcgcccag gtgtaccgcg cctggctcga tcattcggtg ctgttgatcc
     8521 gcggccaggt gctgaccgat gacgatctga ttgccttcag cctgcggttc ggccaactcg
     8581 atttcgcgcc gatccaggag aatggccgcc ggttcgtcga tgggcacccg gagatctacg
     8641 tggtgtccaa cgtgatcgag aacggtgtcg cgatcggctc gctgggggca ggcgaggcag
     8701 tgtggcacac cgacatgtcg tacctggagg acccacccaa ggcgagcatg ctgtatgcga
     8761 tcgaggtgcc accttcgggg gggaataccg ggttcacctc gatgtatcgg gcctatgagc
     8821 tgctgccgga cgagttgaag cggcggctgg tggggctacg ggtgaagcac gacggta
//
LOCUS       DATGEM010000013         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1596580, whole genome shotgun sequence.
ACCESSION   DATGEM010000013 DATGEM010000000
VERSION     DATGEM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596580"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VKQ27_00265"
     CDS             <1..529
                     /locus_tag="VKQ27_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLY87392.1"
                     /translation="LVLMADHELNASTYATRVVASTRAPLGACVLGGLAALVGPLHGG
                     VTNELRAFLADPAVVADPAGAVAIRLARGERVPAFGHPLYPDGDPRADALIARLPPRG
                     PALRLLEAMTAATGVPPNIDFALVVLEQRLHLPAGAAFALFAIGRSVGWIAHALEQWQ
                     SGKLIRPRALYPGDG"
     gene            complement(526..1095)
                     /locus_tag="VKQ27_00270"
     CDS             complement(526..1095)
                     /locus_tag="VKQ27_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLY87393.1"
                     /translation="MRHYAVLAAAGLVAAGMLAPVSGWAQSNPSSNEILKSLTPTGTS
                     GATRGIRVGPSAGGSTGGGSSGASAQAAKPPAVNLTVQFATGSADLTPAARQVLDNLG
                     KAITDKTLTTYHFRVEGHTDTVGTKEGNQALSQHRAQAVVDYLATNFHVDTSKLQPIG
                     MGEDGLLIHTPDQTNEPRNRRVEVVNIGS"
     gene            complement(1195..2997)
                     /locus_tag="VKQ27_00275"
     CDS             complement(1195..2997)
                     /locus_tag="VKQ27_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HLY87394.1"
                     /translation="MRSLGEFLKDAYRLSQPYFTRSNEKWSAWGLLIAIIVMNLSLVG
                     MSVILNFWHREFYNALQDKDWRAFVELLTWYRFTPSGLMPGFCELAAVWIAIAVYSVY
                     MNQWLQIRWRNWMTTHFLDEWLSDRAYYHISLGTDRAAIGTDNPDQRIAEDLRDFTRS
                     TLVLGLDLLSNVVTLFSFLSILWVLSGSISIFGISIPGYMVWVAVIYAVAGSWLTHLV
                     GRPLAALNFRQQRVEADFRYALVRVRENVEGIALYGGEDEEKGLLKHRFSAVIGNWWA
                     IMRRLRWLNALTSGYAQLASIFPIVVAAPRYFFGSMPLGGLMQTVGAFGRVQDALSWF
                     INQYAANNPNEVSLVRYRSIVERLTTFDRAVEITRAAAEKLVLSDSPDGSVRMRDLTI
                     SLPTGAKLLSDAEVILQPGHSVVITGRSGSGKSTLFRTLAGIWPFGSGQVQRPIARSM
                     FLPQRPYIPLGTLRHVVSYPSEPNVYGRDAIAAALADVGLPSLIPRLDQEENWPQLLS
                     GGEQQRLALARALLAKPDWLFLDEATASLDPEAEDELYRMLRERLPGTTLVSIAHRPS
                     VAAFHERRLVFQREEGKSGTLVAMELAPVSGGNG"
     gene            complement(3005..3487)
                     /locus_tag="VKQ27_00280"
     CDS             complement(3005..3487)
                     /locus_tag="VKQ27_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HLY87395.1"
                     /translation="MTPFAWVLGLFLPACGASGAAGLPPPRPIDMSHLDRPASPNTSL
                     AAPAGFTAAPDVVTPQYAVSAQRLYQDVLAVAASQPRTFLASNDQPLLQAQFVVRSAW
                     LNFPDLIVAQVNPAGPDASTLVLYSRSVYGYGDFGVNRARVTTWLADLNRTLTPAKER
                     "
     gene            3575..>4093
                     /locus_tag="VKQ27_00285"
     CDS             3575..>4093
                     /locus_tag="VKQ27_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87396.1"
                     /translation="MSASTCVLKHPVFTTLREPLFRLAETDGTPVMVVKLGERDAVIP
                     LQSLQREFSIPDESDDGRMIALIAQSLDFVAGLRMGDALPPEVLDGGASWTPDEMHLR
                     IAKARLQWQLVTWLNSGTGGDEAELKLDDLVQLTEDPVRRQQVQMAFAKAADSLGLPS
                     RDAVVQLAEELAQ"
BASE COUNT          732 a         1248 c         1368 g          745 t
ORIGIN      
        1 gctggtgctg atggccgatc atgaactgaa tgcctcgacc tatgccaccc gtgttgtcgc
       61 ctcgacccgg gctccgcttg gggcgtgcgt gctgggtggc ctagcggcgc tcgtcgggcc
      121 actgcacggc ggggtcacca acgagttgcg tgcattcttg gccgatccgg cagtcgtggc
      181 cgatcctgca ggcgccgtcg cgatccggct ggcgcgcggc gagagggtgc cggcgttcgg
      241 ccatccgctc tatccggatg gcgacccgcg cgcggacgca ttgatcgcgc gtcttccgcc
      301 acgtggccct gcgttgcggc tgctcgaagc aatgacggcg gccacaggtg tgccgccgaa
      361 cattgacttc gccctggtgg tattggagca gcgcctgcat ctgccagcag gcgccgcctt
      421 tgctctgttt gcgatcggac gctcggtcgg ctggatcgct cacgcactgg aacagtggca
      481 gagcggcaag ctgatccgcc cgcgggcgct gtatccgggc gacggttagc tgccgatatt
      541 caccacctcg acgcgccgat tgcgtggctc gttcgtctgg tcgggtgtgt ggatcagcag
      601 gccatcctca cccatgccaa tcggctgcag cttgctggta tcaacgtgga agttggtcgc
      661 caggtagtcc accacagctt gcgcccggtg ttgcgacagc gcctggttgc cttccttggt
      721 gccgactgtg tcggtgtgtc cctcgacgcg gaagtgatag gtcgtcaggg tcttgtcggt
      781 gatcgccttg ccgagattgt cgagcacctg cctcgcggct ggcgtcagat cggcagagcc
      841 ggtggcgaac tgcacggtca ggtttaccgc agggggcttc gcggcctgcg ccgaggcgcc
      901 gctgctgcca ccaccggtgg acccgccagc ggaggggccg acgcggatgc cgcgtgtggc
      961 gccgctggtg ccggtggggg tcagcgactt caggatctcg ttcgaacttg gattgctctg
     1021 cgcccacccg gacactggag cgagcatgcc ggcggcaacc aggcctgcgg cagccagaac
     1081 ggcgtagtga cgcatgaata ttcctcccac atgagcgagg cgcccgtata ccatgaagca
     1141 cgccgccgtg ggcgcggata tgcactggca aattccggcg gaatcagcgg gctttcatcc
     1201 atttccgccg ctgactggcg ccagttccat cgcaaccaga gtgcctgact tcccttcctc
     1261 gcgttggaat accagccgcc tttcgtggaa cgcggcaacc gatgggcggt gcgcgatcga
     1321 caccagtgtc gtgcccggca gccgctcccg cagcatgcgg taaagttcgt cttccgcctc
     1381 gggatccagg cttgctgtgg cctcgtccag gaacagccag tctggcttgg ccagcagagc
     1441 gcgtgccagc gccaaacgct gctgctcacc gccggacaga agctgcggcc agttctcctc
     1501 ctgatcgagc cgagggatca gtgatggcag cccgacatcg gccagcgccg cagcgatcgc
     1561 gtcacgtcca taaacgttcg gttcgctcgg atagctcacc acgtgacgga gcgtgccgag
     1621 cgggatgtat ggccgctgtg gcaggaacat cgaccgtgcg atcggcctct gcacctgtcc
     1681 gctgccgaac ggccagatgc cggcgagtgt gcggaagagc gtggatttgc cggagccaga
     1741 gcgaccggtg atcaccaccg aatggccggg ctgcagtatg acctcggcat cggacagcaa
     1801 tttcgcaccg gttggcaggc tgatggtcag gtcacgcatg cgaaccgaac catccggtga
     1861 atcgctcagg accagctttt ccgcggcggc gcgggttatt tcgacagcgc gatcaaaggt
     1921 ggtcagacgc tcgacgatgg agcggtagcg aaccagtgac acttcgttcg ggttgttggc
     1981 ggcatactgg ttgatgaacc acgacaatgc atcctgtact cggccgaatg caccaacagt
     2041 ctgcatcagc ccccccagtg gcatcgagcc gaagaaatag cgcggggcgg cgaccacaat
     2101 ggggaagatg ctggccagct gcgcgtagcc ggaggtgagc gcattcagcc agcgcagccg
     2161 ccgcatgatc gcccaccagt tgccaatgac agcgctgaac cgatgcttca gcagcccttt
     2221 ttcttcgtcc tcgccaccat acaatgcgat gccttcgaca ttttcgcgca cgcgcaccag
     2281 ggcatagcgg aagtcggctt cgacgcgctg ctggcggaag ttcaacgcag ccaacggacg
     2341 gccaaccagg tgggtcagcc aactgcccgc caccgcataa atgaccgcta cccagaccat
     2401 gtagccgggg atgctgattc caaagatcga tattgaaccg gacagcaccc aaaggatgct
     2461 gaggaagctg aacagcgtaa ccacgttgga gagcagatcg aggccgagca ccagtgtgct
     2521 tcgggtgaag tcgcgcagat cctcggcgat acgctgatcg gggttgtccg tgccgatcgc
     2581 ggcccggtcg gtacccaggc ttatgtggta gtaagcgcga tcggacagcc actcatcgag
     2641 gaagtgggtt gtcatccagt tgcgccagcg gatctggagc cactggttca tgtacaccga
     2701 gtaaaccgcg atcgctatcc agaccgcggc gagctcgcag aacccgggca tcaggccgct
     2761 tggcgtgaag cggtaccagg tcagcagttc cacgaaagcc cgccagtcct tgtcctggag
     2821 cgcgttgtag aactcgcggt gccagaaatt gaggatcacg gacatgccga cgaggctgag
     2881 attcatcacg ataatggcga tcagcaggcc ccaggccgac catttctcgt tggaacgggt
     2941 gaaatagggc tgggacagcc ggtaggcgtc cttcagaaac tcgccgagac tgcgcatgcg
     3001 ggcgtcatct ttccttggct ggggtgagcg tccggttcag gtccgcgagc caggtggtta
     3061 ccctggcccg attcacgccg aagtcgccat agccgtacac gctgcgcgaa tacagcacga
     3121 gggtgctggc gtctggtcct gccgggttca cttgggcgac aatcaggtct gggaagttga
     3181 gccaggcgct acggacgacg aactgcgcct ggagcagcgg ctggtcattt gacgcgagga
     3241 aggtgcgcgg ctggcttgcg gccacggcga gcacgtcttg gtagagacgc tgtgcggaca
     3301 cggcatattg cggcgtaaca acatcggggg cggccgtgaa gccggccggg gcggctagag
     3361 acgtattggg gctggccggg cggtcaagat ggctcatatc aatggggcgc ggcggcggca
     3421 gccccgctgc gccggaggca ccgcaggccg gcagaaacaa tccaagcacc caggcgaacg
     3481 gcgtcatgct tcctggatgg ccccgatcag gttacgcggc aagtttctga acgcttacgg
     3541 tctgttcacc ggcttgtgtt ctcatggcat tgccatgagc gcatcgacct gtgtgctgaa
     3601 gcatccggtg ttcaccacct tgcgggagcc cctgttccgc ctcgcggaaa ccgatggcac
     3661 gccggtgatg gtggtgaaac tcggcgagcg agatgcggtg atcccgctcc agtcgctgca
     3721 acgggagttc tcgatcccag atgagtcgga cgatgggcgc atgatcgcgc tgatcgccca
     3781 atcgctggac ttcgtggccg gactgcggat gggtgatgcg ctcccgcctg aggtactgga
     3841 cgggggagca agctggacgc ccgacgagat gcacctgcgc atcgccaaag cacggctgca
     3901 gtggcagctc gtcacgtggc tgaattcggg cacgggtggt gatgaggcgg aattgaagct
     3961 ggatgacctg gtgcaactga ccgaggatcc ggtacgccgt cagcaggtgc agatggcctt
     4021 tgccaaggct gccgacagcc tcggcctgcc gtcgcgcgat gcggtggttc agcttgctga
     4081 ggaactggcc cag
//
LOCUS       DATGEM010000014         6258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1133428, whole genome shotgun sequence.
ACCESSION   DATGEM010000014 DATGEM010000000
VERSION     DATGEM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133428"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VKQ27_00290"
     CDS             complement(<1..1279)
                     /locus_tag="VKQ27_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLY87397.1"
                     /translation="MAEADPRHSEKSALEPQTLHDSAAGETPVATNPRRRKALGGVAA
                     AVLLVGGGYGGWYALVGSHHVSTDDAYVGADTAQITPMVAGQVAAVEVEDTQMVRRGQ
                     VLVRIDDSDARIALANAEAQLATTKRKFGQTSATSDALAADVQARADAIASAHAQLSA
                     AQAAFDKASIDLDRRRKLQPSGAVSADELTSAINSYAAAKGDLDHARASLSQAQSNQG
                     SARGSLAANQALIAGTTVATNPDVLAAQAKVDQARLDLSRTVIRAPVDGVVTSRLVQV
                     GQRLAPGADVMKLVPVNQLYVDANFKEGQLTHVRPGQQVTLKSDLYGGNVVYHGRVLG
                     SSGGTGAAFALIPAQNATGNWIKIVQRLPVRVALDPQELKAHPLRVGLSMTVTVDIHD
                     TSGALIASQVRSQPIPVQDSLGHDPEVEARIAQI"
     gene            complement(1272..2768)
                     /locus_tag="VKQ27_00295"
     CDS             complement(1272..2768)
                     /locus_tag="VKQ27_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLY87398.1"
                     /translation="MSKLIRRAPWPAAALTLLLSGCASVPDLGPRPQMREAASFASGQ
                     TLGSDIKASVAVQPTVWPDGRWWLAYNDSQLTSLIDEGLSNAPDLAAAVARLHMAEGY
                     AQQAGAALLPQVDLVGQVNEAKQSYNNGVPKANVPHGWNDTGKVGASFSFDLDLWGKN
                     RAALKAATSDRDAAQVDLAQARLLLATNIVSAYADLARLYAEHDVQMQAVAQRSDIQK
                     LVANRVAIGLDTRAELKQADSAVSAEQATLTAIEESIGLTHNLIAALLGKGPDRGRSI
                     TRPANTSTGRGVPTDVTTNLLGRRPDIVSARERVLAAASRIKVARADFYPSVSLNALI
                     GFQSLGLSNLLTKGSFSGQAGPAVSLPIFHGGAISGRYRVARATYDEAVANYDRTVAD
                     AFHEVADAVTSRAALDTRLGQSRQSLTDAQEAYDIARKRYEGGLSTYLDVLTAENGVL
                     TARRNVADLQARAFTLDVALVRALGGGAISQQTQSALADATSKETPRG"
     gene            2761..3504
                     /locus_tag="VKQ27_00300"
     CDS             2761..3504
                     /locus_tag="VKQ27_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY87399.1"
                     /translation="MDIIGFRTQKYGFNLEIEAACQLKIVPYSTIMKLPPLPAPAAGK
                     REQRKAERRAAIIEIAARSFLEHGYDGTSMSAIAEEMGGSKGTLWSYFTSKEQLFEAF
                     IDTSTSALRAELTAMLDPTGHPRDTLEAFARRFLDRITAPDALRLYRLVIGESERFPQ
                     LGRIFYERAPGTTEPLLADFISHYMDTGALRRDNANHAARMIMSLCAGGLHMRALWRV
                     AEVDAGDIAAEAVRIVDLFLRTYAPAASA"
     gene            complement(3915..4973)
                     /locus_tag="VKQ27_00305"
     CDS             complement(3915..4973)
                     /locus_tag="VKQ27_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLY87400.1"
                     /translation="MPILEFDMLSMRRRGKAQNWYIRGNVTLGGRSVEVAEYSTGTCD
                     KAAASHLMAMREQELREQLLFGPRATAAKAVIADAFEAYLTKPKPPNSTDVVRVGVMN
                     ERIGAMSLGDPKAAWHAFREAYLLNHAPAGQDRYRSLLQAAINVYHDRHGLPACKLKT
                     IAFDNQRIRFLSHADRDRLIGSYAPHVQPIATMFAFQGPRTQEALQLIWGAGGVDMER
                     GTIFFARTKTGNPRTVEIHPRVEALLRPLWLKRGRPSSGHVFLNRKGQPYTDTRDLVV
                     QGGNPLKTAHTNACARAGIADFTVHDWRHHWASHCVMAGIDLITIMRLGGWKSLRMVQ
                     RYAAVDTAHMKEAVMRLR"
     gene            complement(5316..5390)
                     /locus_tag="VKQ27_00310"
     tRNA            complement(5316..5390)
                     /locus_tag="VKQ27_00310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5356..5358),aa:Val,seq:gac)
     gene            complement(5453..5830)
                     /locus_tag="VKQ27_00315"
     CDS             complement(5453..5830)
                     /locus_tag="VKQ27_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87401.1"
                     /translation="MIRILLAEDDRSMREYLARALDKVGYSVVAVDCGTAALPLLEAE
                     RFDLLLTDIVMPEMDGIELAQRAARMAPDMRVMFITGFAAVALKGGITHPNAKVLSKP
                     FHLRDLVMEVDRLFGVGTMAGKP"
     gene            6012..>6258
                     /locus_tag="VKQ27_00320"
     CDS             6012..>6258
                     /locus_tag="VKQ27_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HLY87402.1"
                     /translation="MAENPTEPNVYARLGQIGARSPLIIAVPHAGRFYPPEMLELARL
                     PQHALETIEDRYADLLITDAVAAGAVAIVARTARAYVD"
BASE COUNT         1124 a         2048 c         1993 g         1093 t
ORIGIN      
        1 cgatctgcgc gatgcgcgcc tcgacctccg ggtcatggcc caggctgtcc tgcaccggga
       61 tcggctggct gcgcacctgc gaggcgatca gcgcgccgga ggtgtcgtgg atgtccacag
      121 tcacggtcat cgacaggccg acgcgcagcg gatgcgcctt cagctcctgc ggatcgagcg
      181 cgacgcgcac cggcaggcgc tgcacgatct tgatccagtt gccggtcgca ttctgtgccg
      241 ggatcagcgc gaaggctgcc ccggtaccgc ccgaggagcc gaggacgcga ccgtgataga
      301 caacattgcc gccatagaga tccgacttga gcgtcacctg ctggccgggg cggacatggg
      361 tgagttgccc ttccttgaaa ttggcgtcga catagagctg gttcaccggc accagcttca
      421 tgacgtcggc gcccggcgcg aggcgctgtc ccacctgcac cagacggctc gtcaccaccc
      481 cgtcaacggg cgctcggatc acggtacgcg acaaatccaa gcgcgcctga tccaccttgg
      541 cctgcgcggc cagcacgtcg ggattcgtgg cgacggtcgt gccggcgatc agcgcctgat
      601 tggccgccag actgccgcgc gcgctgccct gattggattg ggcctgcgac aagctggccc
      661 gcgcatggtc aaggtccccc ttcgcagccg cataggagtt gatcgcggaa gtcagttcgt
      721 cggctgagac tgccccgctc ggctgcagct tacggcggcg gtcgagatcg atgctcgcct
      781 tgtcaaaggc agcctgtgcg gccgaaagtt gcgcgtgcgc gctggcaatg gcatcggctc
      841 gcgcctgcac atcggcggcc aaagcatcgc tggtagccga tgtctggccg aacttgcgct
      901 tggtcgttgc aagctgcgcc tcggcattgg ctaatgcgat gcgggcgtcg ctgtcatcta
      961 tgcgcacgag cacctgaccg cgacggacca tctgcgtgtc ctccacctcg accgctgcca
     1021 cctggccggc aaccattggg gtgatctgcg cggtatcggc gccgacgtaa gcgtcgtcgg
     1081 tgctgacatg atggctcccg acgagcgcat accagccgcc atagccgccc cccaccaaca
     1141 gcaccgcggc agccacgccg cccaacgcct tgcgccggcg gggattggta gcgacgggag
     1201 tttcgccggc cgcactgtcg tgtagcgtct gcggctctaa tgcggatttt tcagagtgac
     1261 ggggatcggc ttcagccacg aggggtctcc ttgctggtcg cgtctgcaag cgcggactgc
     1321 gtctgttggg aaatggcacc gccgcccagc gcgcgtacga gcgcgacatc gagcgtaaag
     1381 gcgcgagcct gaaggtcggc gacgttgcgc cgggcggtaa gaacgccatt ctctgccgtg
     1441 agcacgtcga gataggtgga aagcccgccc tcatagcgct tgcgcgcgat gtcataggct
     1501 tcctgtgcgt cggtgagtga ctggcgcgac tggccaaggc gtgtgtcgag cgccgcacga
     1561 ctggtcaccg catcggccac ctcgtggaaa gcgtcagcga cggtacggtc gtaattcgcc
     1621 accgcctcgt cgtaggtggc gcgcgcgaca cgatagcggc ccgaaatcgc gccaccgtgg
     1681 aaaatgggca ggctgaccgc cgggcctgct tgaccggaaa acgatccttt ggtcagcaaa
     1741 ttgctgaggc ccagtgactg aaagccaatc agcgcgttca gactgactga gggatagaaa
     1801 tccgcccgcg ccaccttgat gcgcgaggct gcagccaata cccgctcgcg ggctgatacg
     1861 atgtcgggcc gacgtcccag gagattggtc gtgacgtcgg tgggaacgcc gcgccctgtg
     1921 gaagtgttcg ccggacgggt aatcgaccga ccacgatcag ggcccttgcc caacagcgcc
     1981 gctatgaggt tgtgggtgag accgatcgac tcttcgattg cggtaagcgt cgcctgttcc
     2041 gccgaaacag ctgaatccgc ctgcttcagc tcagcacgag tgtcgagccc gatcgcgaca
     2101 cggttcgcaa ccagcttctg gatgtcgctg cgctgcgcca ctgcctgcat ctgcacgtcg
     2161 tgctcggcat agagccgggc cagatcagca taggcactga cgatattggt cgccagcaac
     2221 agccgcgcct gagcgagatc cacctgcgcg gcgtcgcggt ccgaggtcgc cgccttcagc
     2281 gccgcgcggt tcttgcccca caggtcgaga tcgaagctga agcttgctcc gactttaccc
     2341 gtatcgttcc agccgtgcgg gacgttcgct ttgggaacgc cgttattgta gctctgcttg
     2401 gcttcattca cctgtccgac gagatcgact tgcggcagaa gcgcagcgcc ggcctgttgg
     2461 gcatagcctt cggccatatg cagccgcgcg accgccgccg ccaggtcggg cgcgttggag
     2521 agcccctcat cgatcaggga ggtgagctgg gagtcattat aggcgagcca ccaccggcca
     2581 tccggccaga ccgtcggctg cacggccacg cttgccttga tatccgagcc cagggtctgc
     2641 cctgaggcga aggacgccgc ctctctcatt tgcggacgcg gcccaagatc aggcacggag
     2701 gcacatccgg acaacagcaa ggtcagcgcg gcagcgggcc agggcgcgcg ccgaatcaat
     2761 ttggacataa tcggtttccg tactcagaag tacggcttta atctggaaat agaggccgct
     2821 tgtcaattaa aaattgtacc ctatagtacg attatgaaat tacctcccct tcccgcgcca
     2881 gcggccggca agcgcgagca gcgcaaagcc gagcgccgcg ccgccatcat cgagatcgcg
     2941 gcacgatcct tcctcgaaca tggctatgac ggcacttcga tgtcggcgat cgccgaggaa
     3001 atggggggat cgaagggaac gctctggagc tacttcacct ccaaggaaca gctgttcgag
     3061 gcgttcatcg acacttcgac ctcggctctg cgcgcagaac tgacggccat gctggatccc
     3121 accggccatc cacgagacac gctggaggcg tttgcgcggc gctttctaga tcgcatcacc
     3181 gcgcctgatg ccctgcgcct ctatcgcctc gtgatcggcg agagcgaacg ctttccgcaa
     3241 ctgggccgca tcttctacga gcgcgcgccg ggtaccaccg agccgctgct ggctgatttc
     3301 atcagccatt acatggatac tggagcgcta cgacgggaca atgccaatca cgcggcacgg
     3361 atgatcatga gcctgtgtgc cggcggcttg catatgcgtg ctctgtggcg cgtggccgag
     3421 gtcgatgccg gcgacattgc cgccgaggcc gtccgcattg tcgatctctt cctgcgcacc
     3481 tatgcgccgg ccgcatccgc ctgagatcgg tatcggaaac tcagcgcggg catgccccct
     3541 ggtcaggacg cggaaagttg ctcgatcgcg cccgcacaga aggcatcgga cagttcccag
     3601 tcacccgccg ttgtcaggaa ggcagaggta aaccagcgcc gggactgagc cggtcatcac
     3661 cgccgttccg cgcaagcgca cgggcccgat atgatcatcc caaccgcgcg acgaccccct
     3721 gttcgccgaa ctgttggcaa taaccatccg gtcaagccgc tcgggagcat gcagcgccag
     3781 ccattggcca accatgccgc ccagcgccgg acaagtccgt ggaacagggc ggccgcgaga
     3841 tgcgcgagga tcaggagcag gaagccgtag gccagcacgc ggtcacggcg ctcgatatct
     3901 gcgcccgtag acgcctaacg gagcctcatc actgcctcct tcatgtgggc ggtgtcgaca
     3961 gcggcgtagc gttggaccat ccgcagcgat ttccatccgc cgagccgcat gatggtgatg
     4021 agatcgatcc ccgccatcac gcagtggctg gcccagtgat gacgccagtc gtggacggtg
     4081 aagtcggcga ttccggcccg cgcgcaggca ttggtgtgcg cggtcttgag cggattaccg
     4141 ccctggacga ccaggtcgcg ggtatcggta tagggttggc ccttgcggtt gaggaagaca
     4201 tggcctgaac tgggtctgcc gcgcttgagc cagagcgggc gcagcagcgc ctcgacgcgg
     4261 ggatggatct cgacggtgcg cgggttgccg gtcttggtgc gcgcgaaaaa gatcgtgccg
     4321 cgctccatgt cgacaccccc tgccccccag atgagctgca acgcctcctg ggtgcgcggt
     4381 ccctggaagg cgaacatcgt cgcgatcggc tggacgtggg gcgcatagct gccgatcagc
     4441 cgatcgcggt cggcatggct gaggaagcgg atgcgctggt tgtcgaacgc gatggtcttg
     4501 agcttgcatg ccggcaggcc atggcggtca tgatagacgt tgatcgccgc ctgcagcagc
     4561 gagcggtagc ggtcctgacc ggcaggcgca tggttgagga gataagcctc gcggaaggcg
     4621 tgccacgcag ccttcggatc gccgaggctc atcgccccga tccgctcgtt catcacgcct
     4681 acccgcacca catcggtcga gttgggcggc ttgggcttgg tcagataggc ttcgaacgcg
     4741 tccgcgatca ccgccttcgc cgccgtcgcc ctgggcccga acagcagctg ctcgcgcagc
     4801 tcctgctcgc gcatcgccat caggtgcgat gccgcagcct tgtcacatgt ccccgtacta
     4861 tattctgcga cctcgacgct ccgtcccccg agcgtcacat tcccgcgaat gtaccagttc
     4921 tgcgccttcc cgcggcgccg catgctgagc atatcgaatt ccaagatcgg cacggtgcga
     4981 tctcgacgga gcgcgaagct gcgccccgga aatcgctccc agggttcaga atgacgacca
     5041 ttcccgcagg acggtcgagc gtgtcgaagg aggaccatca ggtcgcgacc actgtgccgc
     5101 cgagtggggc ggatcattcc ccgccctgac gcgcaggacc gcccactcga ttcgagcccc
     5161 ggaggacacc tgcccagccc gtgcccagaa tatgcccaaa catagtggca aaagggggcg
     5221 aaatccggcc cgatatggca cattccggca ccggaaccgg tccattccgg ataaagaaaa
     5281 gccccggaaa actgagcgtt ttccggggct tatcatggtg ggcgtggcaa ggattgaact
     5341 tgcgacccct gcgatgtcaa cacagtgctc taccactgag ctacacgccc gccgtgggag
     5401 gggcctctac ccagcatgcc cgaggatgac aagcaccttg tgacagtttt tttcagggtt
     5461 tccccgccat cgtgcccacg ccgaacagcc gatcgacctc catcaccagg tcgcgcaggt
     5521 ggaaaggctt ggagagcacc ttggcattgg ggtgcgtgat gccacctttc agcgccaccg
     5581 ctgcgaagcc cgtgatgaac atcacgcgca tgtcgggcgc catgcgggct gcgcgttggg
     5641 ccagctcgat gccatccatc tcgggcatca cgatgtctgt gagcaggagg tcgaagcgct
     5701 cggcctccag cagcgggaga gcggcggtgc cgcaatcgac ggcgacgacc gagtagccca
     5761 ccttatccag cgcacgcgcc agatattcgc gcatcgatcg gtcgtcttct gccagcaata
     5821 tcctgatcat cttatcccac ttatcccggc tggcactcac tggcgcgact ggaacgagcg
     5881 actgcggccc gaagctccgt gtggttgcga gagcgacccc gatcgggggg accaccatcg
     5941 cgctccggca ctgcgattcg gtaagcagat gggccattgc ccgccgctca ccgactgctt
     6001 agcagaatag catggccgag aaccctacgg aaccgaacgt ctatgccagg ttgggacaga
     6061 tcggggcgcg ctcgccgctg atcatcgccg tcccgcacgc cgggcgattc tacccgcccg
     6121 agatgctgga gctcgcccgc ctcccgcagc acgcgctgga aacgatcgag gaccgttatg
     6181 ccgacctgct gatcaccgat gcggtggcag ccggcgcggt ggcgatcgtc gcgcggaccg
     6241 ccagagccta tgtcgacc
//
LOCUS       DATGEM010000015         6836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1595935, whole genome shotgun sequence.
ACCESSION   DATGEM010000015 DATGEM010000000
VERSION     DATGEM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595935"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VKQ27_00325"
     CDS             complement(<1..1784)
                     /locus_tag="VKQ27_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY87403.1"
                     /translation="MSAQGRSSDIWYDRWPGYVLAIGAVTIATAVRAVLEPTLGQRSA
                     YLLFCPAILVTAGIGGSGPGITAIAVSTAAASLFAENLNSQASLIETLLFVGTAAGIV
                     LFAAQISVWRQHASDSRSAALASHDRAIQLSRELELLIDGALNYAIYMLDPSGRVAIW
                     NLGAERILGWSEGEILGRHSSIFYQHDEIVAGKPEGDLTRALAEGRVEEEGWRLRKDG
                     GGFLASVTITALFNGDGHHVGFGKVVRDVTEARASEALIEASEMQLRSILATMPEAMV
                     VIDRKGAITSFSVAAEQLFGYSQVDVIGRNVRMLMPEPYRSDHDGYVARYRETGERRA
                     LGMRRRVMGLRRDGTTFPLELSIGESFGGGQQVFTGFIRDLSLQEETEARLQSLQSEL
                     VHVSRVSAMGTMASTLAHELNQPLTAVTSYVEASRDLFQTNRAEDRALLGETLDQAVG
                     QALRAGSIVRRLREFVARGDTDKAVEDVPALVTEAAALGFAGAAESGVRHECIWPDGL
                     GPVLADRIQIQQVLINLMRNAVEALVPRGGGTVTIRASQSAENIHIIVADDGPGLDPK
                     IASRLFGAFHTTKRTGMGLGLSICRTIV"
     gene            2313..2486
                     /locus_tag="VKQ27_00330"
     CDS             2313..2486
                     /locus_tag="VKQ27_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLY87404.1"
                     /translation="MIMNLWKDESGAAAAEYALILAIVGTGIAAAAFLLGTTISNAMN
                     NASNCIKSTTGTC"
     gene            2550..2723
                     /locus_tag="VKQ27_00335"
     CDS             2550..2723
                     /locus_tag="VKQ27_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLY87405.1"
                     /translation="MIIQLWKDEAGAAAAEYALILAIVGTGIALAAFTLGKTISNAMN
                     NASNCIKSTTGTC"
     gene            2846..3718
                     /gene="cpaB"
                     /locus_tag="VKQ27_00340"
     CDS             2846..3718
                     /gene="cpaB"
                     /locus_tag="VKQ27_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HLY87406.1"
                     /translation="MLAFAIVFGLAATFIANTYISGVEHRASAARVGFVNVIAARVPM
                     DFGTEITPDNVRMISLPTNVIPQGAFNSLLQLLPGSTRRVALRPISANEPILSSKISG
                     QGGRASISAVLRPDMRATAVRVSDVAGVGGFVLPGDTVDVLITREPNGAPGQSHDQIT
                     DVLLQGVRVLATDQNANQSANQPTVVKTATLEVTQLDAQKLALAQQVGTLSLVLRNVA
                     EKTEDQVKTVNIADLRDGVAHGAPTPVAQPARPRPSRPTVHVHHAVSAPALQTIQIVR
                     GVAPTSYEVSSHVE"
     gene            3708..5180
                     /locus_tag="VKQ27_00345"
     CDS             3708..5180
                     /locus_tag="VKQ27_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HLY87407.1"
                     /translation="MSNDTMRRRALAVAILAASAAFAPAPGALLRALPVAGALQPDSV
                     EVPLNKSQVVNAQRPFTRAMVGSSDIADILPLSAQSLYVLGKKMGTTSVTLYDRSGTV
                     IAVIDVAVGPDVVSLRRQLSELLPNEPISARISNDSVVLSGTVSNAPAIDRAKQIAAT
                     YAGDKVVNMMSVGGSQQVLLEVRFSEMNRNVGKQIGFNHSFLSNKGNAQGSLGDAASS
                     SALLTNQNGKPTIDLKSILNTFGIVAGSYSLGGLNVFSAMDLLEQKGLVKTLAEPNLV
                     ALSGETASFLAGGEFPIPVAQNASVGGGGNGSTGTAITIEFKPFGVSLGFTPTILADG
                     VINLVVTPEVSSLDPSASVTVNGLVIPGLKTRRAKTTVELHDGQSFAIAGLLRSDFQD
                     TVKQIPILGSVPIIGTLFRSTGFQRGETELVMIVTPHIVKPMREADVRLPTDRVGDPK
                     EADLFLKGRTDSAVRNPFDIKGNDPARPATGLQGPVGHEF"
     gene            5190..5465
                     /locus_tag="VKQ27_00350"
     CDS             5190..5465
                     /locus_tag="VKQ27_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87408.1"
                     /translation="MRYLPFLPLILLPLGGCTPIDATLGGALRQNVALQVVNPDPGLP
                     DTWNGSGDHAALAMKRFRTGTVLEPQGITTTSGIRGSSSAGSGSGGY"
     gene            5467..>6836
                     /locus_tag="VKQ27_00355"
     CDS             5467..>6836
                     /locus_tag="VKQ27_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HLY87409.1"
                     /translation="MRLRLGTLGALAKATEGAVAPTIALSLFGLLAAGGIAFDYSRMA
                     SLDTELQDAADHAALAAASQLDGRPNACARARSAATSLVTNGTRFANDGKGSPITITS
                     GSGCGVSSGIPDGILLYQDNAKTIAATSDANAHFVQVTVNTRTANFALTPVVSVFSSG
                     ALSATAFAGVASSICLVPPVMICNPQETGTNTSFNVSGLVGAGLRLVSVGNGGSWAPG
                     NFGYLDTGAGSGASVLREVLGWSVPPGDCVPTTGVTTKPGATTTVTDALNTRFDIYNQ
                     GQSCPSGGTCPASSNSTKDVVRKNGNGKNSCTLGPQGWQVSTKPYLPSSATTPLTLAQ
                     AQGVDSIGYPRDMCHAVSSTGSCAAGKVGDGVWDRNAYFYVNYGWGNASYGGSPTWQT
                     LTGLGASTTRYQAYQWELAHAGQTVGGKKILDPAGRAVAGGNTSYAPAVCASSVITPG
                     PTNVDRR"
BASE COUNT         1250 a         2295 c         2136 g         1155 t
ORIGIN      
        1 tcgacgatcg tccggcaaat ggaaaggcca agccccatgc ctgtccgctt ggttgtatgg
       61 aaggcaccga acagccggct ggcgatcttg gggtcgagcc cgggcccgtc atccgccacg
      121 atgatgtgga tgttttcggc ggattgcgac gccctgatcg tgacggtgcc gccgccacgc
      181 ggaaccagag cctccacggc gttgcgcatg agattgatga gcacctgctg gatctggatg
      241 cgatcggcca gcaccgggcc caggccatcg ggccagatac attcgtggcg caccccgctt
      301 tccgcggccc cggcaaagcc aagcgcggca gcttcggtga cgagcgccgg cacgtcctcg
      361 acagccttgt cggtgtcgcc gcgcgccacg aattcacgca gtcggcggac gatgcttccc
      421 gcgcgcaggg cctggccgac cgcctggtcg agcgtttcgc cgagcagggc gcgatcctcg
      481 gcccggttcg tctggaaaag atcccgcgaa gcctcgacat aactcgtgac cgccgtcagc
      541 ggctggttga gctcgtgcgc cagcgtggag gccatcgtgc ccatcgcgct cacgcgggag
      601 acatggacca gttcggattg cagggactgg aggcgggcct ccgtctcctc ctggagcgat
      661 agatcgcgga tgaagccggt gaagacctgc tgcccaccgc cgaacgattc cccaatcgac
      721 aactccagcg ggaaggtcgt tccgtcacgg cgcaggccca tcacccgacg gcgcatgccg
      781 agcgcccgcc tttctcccgt ctcccggtag cgggcgacat agccgtcatg gtcggagcga
      841 tagggctcgg gcatcagcat gcggacattc cgtccaatca catctacctg cgaatagccg
      901 aacaattgct cggcggcgac gctgaacgac gtgatcgcgc ctttgcgatc gatcaccacc
      961 atcgcctccg gcatggtggc aaggatcgat cgaagctgca tctcgctcgc ctcgatcagc
     1021 gcttcggagg cacgtgcctc ggtgacgtcg cgaaccacct tgccgaagcc gacatggtgt
     1081 ccgtcaccat tgaacagggc ggtgatcgtc acgctcgcaa ggaagccgcc gccatccttg
     1141 cggagccgcc agccctcttc ctcgacccgg ccttcggcga gcgcccgggt gaggtcgcct
     1201 tcgggttttc cggcgacgat ctcgtcgtgc tggtagaaga tcgagctgtg gcgaccgagg
     1261 atttccccct ccgaccagcc cagaatgcgc tcggcaccca gattccagat ggcgacccgg
     1321 cccgatgggt cgagcatgta gatcgcgtag ttcagcgcgc cgtcgatcag cagctccagt
     1381 tcccgtgaga gctggatcgc gcgatcatgg ctggccagcg ccgccgatcg gctgtccgat
     1441 gcatgctgcc gccacaccga gatctgcgcc gcaaacagca cgatgccggc cgccgtcccc
     1501 acgaacagca gagtctcgat cagggaagcc tgactgttga gattctccgc gaacagcgag
     1561 gcggctgcgg tgctgaccgc aatggccgtg atgcccggtc ccgagccgcc tatgcctgcg
     1621 gtcaccaata tcgcggggca gaagagcaaa taagcggaac gctgcccaag cgtcggctcc
     1681 agcacggcgc ggacggccgt ggcaatcgtc accgcgccga tcgcgaggac atatcccggc
     1741 cagcggtcgt accaaatatc gctcgatcgc ccctgcgccg acatccggcc cccgatccgc
     1801 atgagcggga atagtagatc gaattccgtt cgggtgaaat acgtccgcca cccgttcccg
     1861 ccacgacacc ggccgaagga cgatgccgcg tctgtgacct gggactatgg aatctcacct
     1921 agtccaaaat gactagtggc tcatgttgca ggctctgtct cgatcggtgc gcgcctatcc
     1981 ctctggttgc gtgaagcccc catctcccgt tcggcacgag gagtggcatg atcaaatgag
     2041 agcccagcaa tcacaaataa acaaataata acaagaagtt ggtctacgta ttattacgta
     2101 ctggcccatt aggcagcatt ccttccgaaa tggtgatgcc ccaaattgaa ccagtcctgg
     2161 agcggctttg cgccgtaccc acaaagactc tctgtccaag tatagggtgg tgtgaccgta
     2221 atagccccgc cgttccgacc aatggtttcg gaatggttat cacatcgtcg aggctcgtct
     2281 cggcgaatag tgggggaaat tccgtgacca agatgatcat gaacctgtgg aaggacgagt
     2341 ccggcgcagc cgcagccgaa tatgcgctca tcctggcgat tgtcggcacc ggcatcgcgg
     2401 ccgctgcgtt tctgctcggc accacgatca gtaacgccat gaacaacgcc tcaaattgca
     2461 tcaagagcac gaccggcact tgttaagcgc caagtgacgc tcatcgggac cggttgatcg
     2521 caacaagtgg gggaaattcc gtgaccaaga tgattatcca actctggaaa gacgaagccg
     2581 gcgccgccgc cgcagaatat gcgctcatct tggcgatcgt cggtaccggc atcgctctag
     2641 cggcgttcac gctcggaaaa acgatcagca acgccatgaa caacgcctcg aattgcatca
     2701 agagcacgac gggcacttgt taagcgctag gtgacgttca tcgggaccgg ctgacttaaa
     2761 gcgaagccgg tcccacttct cagggggcat ctcctgattt ctatcctccg gggggaggga
     2821 tatggtccaa ggtcgctcac tcatcgtgct cgccttcgcc atcgtcttcg gactggcggc
     2881 gacattcatt gccaacacct acatcagcgg cgtcgagcat cgggccagcg cggcgcgcgt
     2941 cggcttcgtc aacgtcatcg cggcgcgcgt tccgatggat ttcgggaccg agatcacccc
     3001 cgacaatgtg cggatgatca gcctgccgac gaacgtgatc ccgcaaggcg ccttcaactc
     3061 gctgctccag ctcctgccgg gcagcacgcg gcgcgtcgcg ctgcggccaa tctcggcgaa
     3121 cgagccgatc ctcagcagca agatttcggg ccagggcgga cgggcgagca tctccgccgt
     3181 gctgcgcccc gacatgcggg ccaccgccgt ccgcgtgagc gacgtcgccg gcgtcggcgg
     3241 ctttgtgctg cccggcgaca ccgtggacgt gctgatcacc cgcgagccga acggcgcgcc
     3301 ggggcaaagc cacgaccaga tcaccgacgt gctgctccag ggcgtgcgcg tgctggccac
     3361 cgaccagaac gccaatcaga gcgccaacca gccgacggtg gtcaagaccg ccacgctcga
     3421 ggtgacacag ctcgacgccc agaagctcgc gttggcccag caggtcggca cgctcagcct
     3481 ggtgctccgc aacgtcgccg agaaaacgga agaccaggtc aagacggtaa acatcgccga
     3541 tctgcgcgat ggcgtggcgc atggtgcgcc gacgcctgtc gcgcagcccg cgcggccaag
     3601 gccaagccgg cctaccgtcc atgtccatca tgccgtttcg gcgcccgccc tgcagacgat
     3661 ccagatcgtc cgcggcgtcg ccccgaccag ctacgaggtc agcagccatg tcgaatgaca
     3721 ccatgcgccg gcgcgccctc gcggtcgcga tcctggccgc ttccgccgcc ttcgcgcccg
     3781 ctcccggcgc gctccttcgg gccttgcctg tcgccggtgc cctccagccc gactcggtcg
     3841 aggttcccct caacaagagc caggtggtga acgcccagcg ccccttcacc cgggcaatgg
     3901 tcggcagcag cgacatcgcc gacatcctgc cgctctccgc tcaatcgctc tatgtgctcg
     3961 gcaagaagat ggggacgacg agcgtcacgc tctacgatcg ttcgggcacc gtgatcgcgg
     4021 taatcgacgt cgcggtcggc cccgatgtcg tctcgctccg gcgtcaactc tccgagcttc
     4081 tgcctaacga gccgatcagc gcgcgcatct ccaacgactc ggtggtgctg agcggcaccg
     4141 tgtcgaacgc gccggcgatc gatcgcgcca agcagatcgc cgccacctat gcgggcgaca
     4201 aggtggtcaa catgatgtcg gtcggcggat cgcagcaggt gctgctcgag gtccgcttct
     4261 ccgagatgaa tcggaatgtg ggcaagcaga tcggcttcaa ccactccttc ctgtccaaca
     4321 agggcaatgc ccagggctcg ttgggtgatg cagcatcgtc gagcgcgctg ctgaccaacc
     4381 agaacggcaa accgacgatc gatttgaaat cgatcctcaa caccttcggt attgtcgccg
     4441 gcagctattc gctggggggg ctgaatgtct tctcggcaat ggacctgctc gagcagaagg
     4501 gactggtgaa aacgctcgcc gagcccaatc tggtcgcgct gtcgggcgaa accgcctcct
     4561 tcctcgccgg cggcgagttc ccgatcccgg tggcgcagaa tgccagcgtc ggcgggggcg
     4621 gcaatggctc caccggaacg gcgatcacca tcgagttcaa gccgttcggg gtcagcctgg
     4681 gcttcacgcc cacgatcctg gccgacggcg tgatcaacct ggtggtcacc ccggaagtca
     4741 gctcgctcga tccgtcggcc tcggtcacgg tcaatggcct ggtcatcccg gggctcaaga
     4801 cccgccgggc aaagaccacc gtggaactgc acgatggcca gtccttcgcg atcgcagggc
     4861 tgctgcgcag cgatttccaa gacacggtga agcagatccc gatcctgggg tcggtgccga
     4921 tcatcggcac gctgttccgt tcgacgggct tccagcgcgg cgagaccgag ttggtgatga
     4981 tcgtcacccc ccacatcgtg aagccgatgc gcgaggcgga tgttcgtctg ccgaccgacc
     5041 gggtgggcga tcccaaggaa gccgacctct tcctcaaggg ccggaccgac tccgccgtcc
     5101 gcaacccctt cgacatcaag ggcaatgatc ccgcccggcc cgccaccggc ctccagggcc
     5161 cggttggcca cgaattctag gagatgacga tgcggtatct tccgttcctt ccgctcatcc
     5221 tcctgccgct tggcggttgc acgccgatcg acgcgacgct gggcggtgcc ctccgccaga
     5281 atgtcgcgct gcaggtggtc aatcccgatc ccggcctgcc ggacacgtgg aacggcagcg
     5341 gcgatcatgc cgcgctggcg atgaagcggt tccggaccgg gaccgtactc gaaccccagg
     5401 gcatcacgac gacgtcggga atccgcggga gtagcagcgc cggctcgggg tcgggaggct
     5461 attgagatgc gcctgcgcct cggcacgctc ggcgcgttgg cgaaggcgac cgagggcgcg
     5521 gtcgcaccga cgatcgcgct gtcgctcttc ggcctgctcg cggcaggcgg gatcgccttc
     5581 gactattcgc gcatggcctc actcgacacc gaattgcagg acgcggccga tcatgccgcg
     5641 ctggcggcgg caagtcagct cgatggtcgc cccaacgcct gcgcccgcgc gcggagcgcg
     5701 gcgacatcgc tggtcaccaa cggcacacgg tttgccaatg atggaaaagg ctcgccgatc
     5761 acgatcacta gcgggtcagg ctgtggcgtt tccagcggca tccccgacgg catcctcctc
     5821 tatcaggaca atgccaagac gatcgccgcc accagcgatg ccaatgcgca tttcgtgcag
     5881 gtgacggtca acacgcggac ggccaatttc gcgctgacgc cggtcgtctc ggtattctcg
     5941 tcgggggcgc tgagcgcgac cgccttcgct ggcgtggcgt cgtcgatctg cctggtgccg
     6001 cccgtcatga tctgcaatcc gcaagagacc ggcaccaaca ccagcttcaa tgtgtcgggg
     6061 ctcgtgggtg cggggctccg cctggtttct gtcggcaatg gcggaagctg ggcaccgggc
     6121 aatttcggct atctcgatac cggcgcgggc agtggcgcca gtgtgctgcg cgaggtgctg
     6181 ggctggtctg tgccgccggg cgactgtgtg ccgaccacag gtgtaaccac caagccgggc
     6241 gcgacgacga ccgttaccga tgcgctcaac acccgcttcg acatctacaa tcagggccag
     6301 agctgtccga gcggcggcac ctgtccggca tcgtccaaca gcaccaagga tgtcgtgcgc
     6361 aagaacggca acggcaagaa tagctgcacg ctcggtcccc aaggctggca ggtttcaaca
     6421 aaaccctacc taccgtcgag cgcaaccacg ccgctgacgc tggcgcaggc gcagggagtc
     6481 gactcgatcg gttacccgcg cgacatgtgc cacgcggtga gcagcaccgg cagctgcgcg
     6541 gccggcaagg tcggcgacgg tgtgtgggat cgcaacgcct atttctacgt caactatggc
     6601 tggggcaatg cgagctatgg cggcagcccg acctggcaga cgctgaccgg gctcggcgcg
     6661 tccaccacgc gctaccaggc ctatcagtgg gagctcgccc atgccggcca gacggtcggt
     6721 ggcaagaaga tcctcgatcc cgccggccga gcggtggcgg gcggtaacac gtcctatgcg
     6781 cctgcggtgt gtgccagcag cgtgatcacg cccgggccca ccaacgtcga ccggcg
//
LOCUS       DATGEM010000016         8090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1249738, whole genome shotgun sequence.
ACCESSION   DATGEM010000016 DATGEM010000000
VERSION     DATGEM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249738"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VKQ27_00360"
     CDS             complement(<1..344)
                     /locus_tag="VKQ27_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87410.1"
                     /translation="MDLGLKGKKAIVCAASKGLGRACAMSLAREGVDVVITARTAVEL
                     EKTADEIRKATGAKVTAVPGDITTDAGRAAALAACPAPDILVNNAGGPPRGDFREWGR
                     DDWIKAVDANMLT"
     gene            complement(381..863)
                     /locus_tag="VKQ27_00365"
     CDS             complement(381..863)
                     /locus_tag="VKQ27_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87411.1"
                     /translation="MIMMHAWRSRRIIGVIAAIGSLMIALTASERTEAANPDGRPAII
                     LPDVLVGSVQGADAPIDVRFVTEADTQVDLGTLHVRAHKFVGWIDITQRLLQHPRARV
                     GVWGIHLDGGVLPAGQHLVRLSLHDMKGRAVEAIQTIRITTTHGGCSCGAGIASPRGR
                     "
     gene            1046..1534
                     /locus_tag="VKQ27_00370"
     CDS             1046..1534
                     /locus_tag="VKQ27_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HLY87412.1"
                     /translation="MKRNVLLSGLAALAIAAGLSGAPASAQTTTGVKVGALTCDVASG
                     WGFVFGSTRDLKCTYSDNNGSVERYTGHVNKFGADIGYHAGGAVVWAVIAPTSNLAKG
                     ALAGNYGGVTGGAAIGIGAAANVLVGGSGQTVSLQPVSFEGITGLNVAAGIEQVVLTA
                     SE"
     gene            2326..3654
                     /locus_tag="VKQ27_00375"
     CDS             2326..3654
                     /locus_tag="VKQ27_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HLY87413.1"
                     /translation="MPTVTVAGAHGQIVSLSFDTAANAVLAQKLAGAITAGVDNGSIV
                     PAVDSDGTPPPVPSGKTGEWVQTHPGTTILPTGYTAVVDTAPDSTIFGSGDNGESILS
                     SIGNMTFFASSGSGTVVTGGGDNRIVLESSDNGAWQINTGEGDDTIIAQNTGNDTISA
                     GVGKNSIMLGGGSDLVLSTGDDRIVASTGRETISATGMHDVLVYGNASNVFFVTDLGA
                     ATVVGGSGSDTFFGGKGTDLVYGGTGGNNFLMAGTGAATLFGGGNGDQLFGAGSVTQA
                     LHAGAGNETLFGGMGGDTFYGSSGSTQIFGGFGADTFVAGTGAATITSGFTNNLFEFT
                     NGQAGGSESIQGFVSGRDVLDLQGYGKNEVTKALKSQHLTAGGDVITLSDHTTITFAG
                     ITNLTANDFVTSGGSAAGGNPGDGDHHSHGGDGDADDHSRHIRDGIIGHH"
     regulatory      3748..3827
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3878..5092
                     /gene="metZ"
                     /locus_tag="VKQ27_00380"
     CDS             3878..5092
                     /gene="metZ"
                     /locus_tag="VKQ27_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HLY87414.1"
                     /translation="MTNNPSNYRTATRLVHGGVQRSHHGETAEALYLTSGYVYDDAEQ
                     AEATFAGTIEHYQYSRFANPTLTMLEDRLCQIEGAEACRTTATGMAAVNSALLAHLKA
                     GDRVVASRALFGSCHWIVSTLLPKFGIATEFVDCADLGQWRAALSKPAQLVLLETPSN
                     PMLELVDLRGVADLAHAAGAVVVVDNVFATPLLQQPLKFGADVIVYSCTKHIDGQGRV
                     LGGAVLGSKKWINETLQPFIRNTGPAMSPFTAWLLLKGLETLALRVDASCRSAVTLAD
                     FLATQPQVTRVWYPTRPDHPQHALAQKQMTAGGTVVTFEVAGGKDAAFSVMNAFGLIA
                     VSNNLGDSKSLATHPATTTHMRIGAEERAKLDITDGVIRLSVGLEDVDDLKDDLAHAL
                     ATISTATARAAE"
     gene            5158..5562
                     /gene="apaG"
                     /locus_tag="VKQ27_00385"
     CDS             5158..5562
                     /gene="apaG"
                     /locus_tag="VKQ27_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HLY87415.1"
                     /translation="MAENYTKTTRDVRVSVRSFFLADQSEPDESHFVWAYRVKIENRG
                     REPVQLLRRTWQITDARGRTQHVHGAGVVGEQPLLEPGESFEYTSGTPLDTPSGFMVG
                     AYHMVVPSSGENFDVAIPAFSLDSPHQSGRVH"
     gene            5596..6234
                     /gene="folE"
                     /locus_tag="VKQ27_00390"
     CDS             5596..6234
                     /gene="folE"
                     /locus_tag="VKQ27_00390"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLY87416.1"
                     /translation="MNIVTPVARAATDATIDRPTREEAEEAVRTLLRWAGDDPTREGL
                     VDTPGRVARAYEEFFAGYDVDPVGLLERTFEETDGYDEIVLLRDIRLETHCEHHMVPI
                     IGRAHVAYLPYRRVVGISKLARVVEAYAKRLQIQEKLTAQIANTIQEVLEPRGVAVVI
                     EAAHQCMTTRGVHKPGVTMVTSRMLGAFRDNPTTRREVLAMIGNGRGFSAES"
     gene            complement(6315..7370)
                     /gene="prfA"
                     /locus_tag="VKQ27_00395"
     CDS             complement(6315..7370)
                     /gene="prfA"
                     /locus_tag="VKQ27_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLY87417.1"
                     /translation="MNLAPKLDRIAARADELRDMLTQGLSGDAYAKASRELADIEPVV
                     ERIGDLRAAERAQHDAETLMADPEMRELAEAELRELKERIPTLEHELRISLLPKDAAD
                     ERAAILEIRPAAGGDEAALFAADLFGMYQRYAEARGWRWEILEYNDTELGGLKEGIAE
                     ITGRGVFARLKYESGVHRVQRVPTTESQGRIHTSTVTVAVLPEAEEVDVQIDEGDLRI
                     DVYRASGAGGQHVNKTESAVRITHVPSGIVVAMQEEKSQHKNRAKAMKILRARLYEQQ
                     RASLHATRAADRKAQVGTGDRSERIRTYNFPQGRVSDHRINLTLYKIDRVMAGELDEF
                     IDALTAEDQAARLAAEE"
     gene            complement(7367..>8090)
                     /locus_tag="VKQ27_00400"
     CDS             complement(7367..>8090)
                     /locus_tag="VKQ27_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HLY87418.1"
                     /translation="ESRQAYRDALVAYFTEHQGALSEDSLARLDRNPMRILDSKDPGD
                     RKLVADAPTITAYLTEAAAGFYDGVKRYLTQFGVPFRENPRIVRGLDYYGHTAFEFIT
                     TKLGAQDAVGGGGRYDGLVAEMGGPQVAAVGWALGVDRLALLLDEPPPAPAGVAVVPI
                     GETEEKALEIVQSLRRAGVRAEMAYRGNLRRRMERANRTGARAAVIIGEDDVAHGVAQ
                     VKDLRTGEQEAVALGDIAARLR"
BASE COUNT         1456 a         2818 c         2397 g         1419 t
ORIGIN      
        1 gtcagcatgt tggcatcgac cgccttgatc cagtcgtcgc ggccccactc acggaaatcg
       61 ccgcgcggcg gtccacccgc attgttcacc aggatgtccg gcgccggaca cgccgccagc
      121 gccgccgcgc gtcccgcatc ggtggtgatg tcacccggca ccgcggtgac cttcgcgccc
      181 gtcgccttgc ggatctcgtc cgccgtcttt tccagttcaa ccgcagtgcg cgccgtgatc
      241 accacatcga cgccctcccg cgcgagcgac atggcgcagg cgcggccgag gcctttgctg
      301 gcagcacaga caatggcttt cttgcctttg agtccgagat ccatgggtgg ggcttccttc
      361 cgtatcgata ccccacgctg tcatcgcccg cgcggcgagg caatccccgc cccgcagcta
      421 catccgccgt gcgtagtcgt gatgcgaatg gtctggatcg cctcgacagc gcgccccttc
      481 atgtcgtgca gtgacagccg cacaaggtgc tggcccgccg gcaggacacc accgtcgaga
      541 tggattcccc aaacgcccac acgcgcccgc ggatgctgca gaagccgctg agtaatgtcg
      601 atccagccga cgaacttatg ggcccggaca tgcagtgttc cgagatccac ttgcgtgtcg
      661 gcctcggtca caaaccgcac atcgatcggc gcgtcggcgc cctgaacgct gcccacgagc
      721 acatccggca ggatgatcgc cggcctgccg tcagggttgg ccgcctcggt ccgctcgctg
      781 gcggtaagtg cgatcatcag cgagccgatc gcggcaatca ctccgataat tcgtcgggaa
      841 cgccacgcat gcatcatgat cataagccat cctggctggc taaatgggat ctatccacgt
      901 cccctccttt gatccaggtc aatgcgccgc aacgcgcgca cttctagtca tttgccgtcg
      961 atgcgcgggg ggccgacttc aacggcaccc gcaaactggt caggcccatc ggggggctgg
     1021 cattttggga aacaggagcg gcgacatgaa acggaatgta ttgctgagtg gtttggcggc
     1081 gcttgctatc gcagcggggc tgtcgggcgc gcccgcatcg gcccagacca ccactggcgt
     1141 gaaggtaggc gcgctgacct gtgacgttgc aagcgggtgg ggcttcgtgt tcggatcaac
     1201 tcgcgatctg aaatgcacct actccgataa caacggctcg gtcgagcgct acaccggcca
     1261 tgtcaacaag ttcggtgccg acattggcta ccatgctggc ggcgctgtcg tctgggcggt
     1321 gatcgcgcca acaagcaacc tcgccaaggg cgcgctggct ggtaactacg gcggcgtgac
     1381 cggtggcgcg gccatcggca tcggcgccgc ggccaacgtg ctggttggtg gctccggcca
     1441 gacggtctcg ctgcagcccg tgagctttga gggcatcact ggcctcaacg ttgccgccgg
     1501 tatcgagcag gtcgtgctga ctgcctctga ataaggccgt caagcggctt tgcgccggta
     1561 gctgagatgg ggacctcgct tactgtcggc ggaggtcccc atcgcccgct gctcaatgca
     1621 aaactccgtg cagcgcggta gaggcgcatc cgaagttcac atcacgcagc ggaaatgcca
     1681 ttgacgcgac agcacggttt tatttaacgc cgattacgat aaatagagcg gcgtatccgt
     1741 ctggccgagc tcgccgggac ctttggctcc gctattccgt ctcgcacagc cggcccaaag
     1801 ggttaaccca catctcattg agcgggaccg atcgatgctc ccgtcatgcc gggcggaagg
     1861 cacggcagcc acctcgcctc gctgatttcc tgctgaccgg gccgcggcac gcagcaatac
     1921 tcaggcccat cgcggaagcc cgccacagcc cattgcgtgc cgggcttggc tcaatcggcg
     1981 aatgcttcgg ctcgcccgga tgctgttgta tatacccgcc gccctgccca accgacgcgc
     2041 gaagcctcgg acggttcgcc cgcgcgcgat ccatcgacag ggtcgcatgc ccaagctggc
     2101 cattaatgtg ttgttaatgg attcctgcgt taaacacccc aggaagtacc cacctcgccg
     2161 tcacacccaa ttgacgaacg ttattttgac gcggcgcaca agaagcgcag cgcagtacta
     2221 cctaattttt agcgaagttt cccatgcgaa tgacgcattg ctcctggtta aagtcgaacg
     2281 gcgaggcagg ttacttcccg aaccgcaact aggggagacc aagccatgcc gacagttacc
     2341 gtcgctggtg cgcacggcca gatcgtcagc ctcagcttcg atactgcggc caatgcggtg
     2401 ctcgctcaga agctcgccgg cgccatcact gccggcgtgg acaacgggtc catcgtccct
     2461 gcggtcgata gcgatggcac gccgccgccg gtaccgagtg gcaagactgg tgaatgggtc
     2521 cagacgcatc ctggtacgac gatactgccg actggttaca ccgcggttgt cgacaccgca
     2581 ccggactcga caatcttcgg ttccggcgac aacggcgagt cgatcctgtc cagcatcggc
     2641 aacatgacat tctttgccag tagcggttca ggaacggtgg tcacgggcgg cggcgacaac
     2701 cgcatcgtgc tggagagcag cgataacggc gcatggcaga tcaacaccgg cgagggtgac
     2761 gacacgatca tcgcccagaa caccggcaat gacacgatca gcgccggcgt cggcaagaat
     2821 tcgatcatgc tcggcggcgg cagcgacctg gtgctgtcca ccggcgacga caggatcgtg
     2881 gcctcgaccg gccgggaaac catctcggcg accggcatgc acgatgtcct cgtctacggc
     2941 aacgcgagca acgtcttctt cgtcacagat ctgggggccg ccacggtcgt cggcggctca
     3001 ggcagcgata cattcttcgg cggcaagggc accgacctgg tctacggcgg caccggcggc
     3061 aacaacttct tgatggcggg cacaggcgcc gcaaccctgt tcggtggtgg caacggcgac
     3121 cagctctttg gcgccggcag cgtcacacag gcgctgcacg caggcgccgg caacgagacc
     3181 ctgttcggcg gcatgggcgg tgacaccttc tacggcagct ccggctccac ccagattttt
     3241 ggcggctttg gcgccgatac cttcgtcgcc ggcactggcg ccgcgaccat caccagcggg
     3301 ttcaccaaca acctgttcga gttcaccaat ggccaggccg gcggctccga atcaatccag
     3361 ggcttcgtca gcggccgcga tgtgctcgac ttgcaggggt atggcaagaa cgaggtgacc
     3421 aaagcattga agtcgcaaca cctgaccgca ggcggcgatg tgatcacgct atctgaccac
     3481 acaacgatca cctttgccgg catcaccaat ctgacggcca atgacttcgt caccagcggc
     3541 ggcagcgccg ccggcggcaa tcctggcgat ggcgatcatc attcccatgg tggcgacggc
     3601 gacgcagatg atcacagccg tcatattcgc gatggcatca tcggccacca ttgatgcatg
     3661 aagggccata cggagaccct ggcaccttcc gtatggcccc tttcttgacg ccgcccctga
     3721 cccggacgta gtttccgctc cgtcggcatt gccgaccctc gtgatttgtc cggccggatt
     3781 gcggcgaggt gaaacaagcg cgctaaagag gccagcgagc agcgacaggc tgcaagctgc
     3841 tccatcggtc gggccaactg cttgaggggc ccagacagtg accaacaatc cttccaacta
     3901 ccgcaccgca acccgcctcg ttcacggcgg cgtccagcgc agccatcacg gcgaaaccgc
     3961 cgaggcgctc tatctcacct ccggctacgt ctacgacgac gccgaacagg ccgaggccac
     4021 cttcgccggc acgatcgagc actaccagta ttcacgcttc gccaacccca ccttgacgat
     4081 gctcgaggac cggctttgcc agatcgaggg cgccgaggcc tgtcgcacca ccgccaccgg
     4141 catggcggcg gtgaactccg cgctgctcgc acatctgaaa gccggtgacc gcgtggtggc
     4201 gtcgcgcgcg ttgttcggct cctgccactg gatcgtttca acgctgctgc ccaagttcgg
     4261 aatcgccacc gagttcgtcg attgcgccga tctcgggcag tggcgcgcgg cactctcgaa
     4321 gcccgcacag ctcgtgctgc tggagacgcc gtcgaacccg atgctggaac tggtcgatct
     4381 gcgcggcgtc gccgacctgg cgcacgccgc tggcgcggta gtcgtggtcg acaatgtgtt
     4441 cgccacgccg ctgttgcagc agccgttgaa attcggcgcc gatgtcatcg tctattcatg
     4501 caccaagcac atcgacggcc agggccgcgt gctcggcggc gcggtgctgg gcagcaagaa
     4561 gtggatcaac gagacgcttc aaccgttcat ccgcaatacc ggtccggcaa tgtcgccgtt
     4621 caccgcatgg ctgctgctga aaggcctcga gacgctggca ctccgcgtcg atgcatcatg
     4681 ccgcagcgcg gtgacgcttg ccgacttcct cgccacccag ccccaggtga cgcgcgtgtg
     4741 gtatcccacc cgcccagatc atccgcagca tgcgctcgcg cagaagcaga tgacggctgg
     4801 cggcactgta gtgacgtttg aagtggcagg cggcaaggac gcggcattca gcgtgatgaa
     4861 cgcattcggc ctgatcgccg tttccaacaa cctcggcgat tccaagtcgc tcgcaacgca
     4921 tccagccacc accacgcata tgcgcatcgg tgccgaggaa cgcgcgaagc tcgacatcac
     4981 cgatggcgtg atccgcctgt ccgtcgggtt ggaagacgtc gacgacctga aggacgacct
     5041 cgctcatgca ctcgcaacga tcagtacagc gaccgcgcga gccgcagaat agcccgcact
     5101 ggacaacgca cacgctctcg ccttgaattc acccactcaa ggctactcgg ttgtgccatg
     5161 gcggaaaact acaccaagac cacgcgtgac gtgcgtgtct ccgtccgttc gttctttctg
     5221 gccgatcagt cggagccaga cgaaagccat tttgtctggg cgtatcgcgt gaagattgag
     5281 aaccgcggcc gcgagcccgt tcagctcctt cgccgcacct ggcagatcac cgacgcgcgc
     5341 ggccgcacgc agcatgtgca cggcgcgggt gtcgtcggtg aacagccgct gctcgaaccg
     5401 ggcgaaagct tcgaatacac gtcaggtacg ccgctcgaca cgccctctgg atttatggtg
     5461 ggcgcctacc acatggtggt accgtcgtcc ggcgagaact tcgacgtggc cattccggcg
     5521 ttcagtctgg acagccctca ccagagcggt agggtgcact aaaggcccca gcaaacccgg
     5581 ggagggagta ctaacgtgaa catcgtcacg cccgttgccc gcgcggcaac ggatgccaca
     5641 atcgatcgac cgacgcgtga ggaggcggaa gaagcagttc gcactctctt gcgctgggcc
     5701 ggcgatgatc caacgcgcga aggtctcgtg gatacaccag gccgggtcgc gcgcgcctac
     5761 gaggagttct tcgccggcta cgatgtcgat ccggtcggat tgctggaacg cacgttcgag
     5821 gagaccgacg gctacgacga gattgtcctg ctgcgtgata tccgcctgga aacccattgc
     5881 gagcaccaca tggtgcctat catcggccgc gcgcacgtgg cgtatctgcc ctatcggcgc
     5941 gtcgtcggca tcagcaagct ggcacgtgtc gtggaagcct atgcgaagcg cctgcagatc
     6001 caggaaaagc tcaccgcgca gatcgccaac acgatccagg aagtcctgga accgcgtggc
     6061 gttgccgtcg tgatcgaggc cgcccaccag tgcatgacca cacgcggcgt ccacaaaccc
     6121 ggcgtgacga tggtcacaag ccgcatgcta ggcgccttcc gcgacaatcc caccacccgc
     6181 cgcgaagtgc tggcgatgat cggcaacggc cgcggcttca gcgcagaaag ctgaacgact
     6241 gtcattgcga gcgctgcgaa gcaatctcga acgagtttac ttcgcgtgcg ccccgagccg
     6301 acatcagcgc cccatcactc ctccgccgcc agccgtgccg cctgatcctc agcggtcagc
     6361 gcatcgatga actcgtccaa ctcgccagcc atcacccgat caatcttgta cagcgtgaga
     6421 ttgatccgat gatccgacac gcgcccctgc ggaaaattgt aggtacggat ccgctctgac
     6481 cgatcccccg tgccgacctg cgccttgcgg tccgccgccc gcgtcgcatg caggctggcg
     6541 cgttgctgct cataaagccg cgcccgcagg atcttcatcg ccttcgcacg atttttgtgc
     6601 tggctcttct cctcctgcat cgccaccacg atgccactcg gcacatgcgt gatacgcacc
     6661 gcgctttccg tcttgttgac gtgctgtccg ccggcacccg acgcgcgata cacatcgatc
     6721 cgcaggtcgc cctcatcgat ctgcacgtcc acttcttccg cctcgggcag caccgcgacg
     6781 gtgactgtgg atgtgtgtat acgcccttgg ctctccgtcg tcggcacccg ctgcacgcga
     6841 tgcacgcctg actcatactt cagccgcgcg aacacaccgc gcccggtgat ctcggcgatg
     6901 ccttccttca gcccgccgag ctcggtgtcg ttgtattcca gtatctccca gcgccagccg
     6961 cgcgcttccg cataacgctg gtacatcccg aacaggtccg ccgcgaacaa cgccgcctcg
     7021 tcgcctcccg ccgccggccg tatctccagg atggccgcac gctcgtccgc cgcatccttc
     7081 ggcagcagcg agatacgcag ctcatgctcc agcgtcggga tgcgctcctt cagttcgcgc
     7141 aactcggcct cggccagttc ccgcatttcc ggatcggcca tcagcgtctc ggcatcgtgc
     7201 tgcgcccgct cggccgcgcg cagatcgccg atgcgttcca ccaccggttc gatatccgcc
     7261 agctcgcgcg aggccttcgc ataggcgtcg cccgacagcc cttgcgtcag catgtcccgc
     7321 aactcgtcgg cccgcgccgc gatgcggtcc aacttcggcg ccaggttcat cgcagccgcg
     7381 cggcgatatc ccccagcgcc accgcctcct gttcgcccgt ccgcaggtcc ttcacctggg
     7441 ccacgccatg cgcgacatcg tcctcgccga tgatcaccgc ggcccgcgcg ccggtccggt
     7501 tggcacgctc catgcgccgc cgcagattgc cgcgataagc catctcggcc cgtaccccgg
     7561 cgcggcgcag cgactgcacg atctccagcg ccttctcctc cgtctcgccg atcggcacca
     7621 ccgcgacgcc cgcgggcgcc ggcggcggct catccagcag cagcgccagc cgatcgacgc
     7681 cgagcgccca gccgacggcc gctacctgcg gcccgcccat ctccgccacc aacccgtcat
     7741 accgcccacc accacccacc gcatcctggg cgccaagctt ggtggtgatg aactcgaacg
     7801 ccgtatgacc gtagtagtcc agcccgcgca cgatccgcgg gttctcccgg aacggcacgc
     7861 cgaactgcgt cagataccgc ttcaccccgt catagaaccc cgccgcggcc tcggtcaggt
     7921 acgccgtgat cgtcggtgca tccgccacca gcttgcggtc gccgggatcc ttgctgtcca
     7981 ggatgcgcat cgggttgcga tccagccgtg ccaggctgtc ctccgacagc gcgccctggt
     8041 gctcggtgaa atacgccacc agcgcgtcgc gataggcctg ccggctttcc
//
LOCUS       DATGEM010000017         6179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_678199, whole genome shotgun sequence.
ACCESSION   DATGEM010000017 DATGEM010000000
VERSION     DATGEM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678199"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VKQ27_00405"
     CDS             <1..706
                     /locus_tag="VKQ27_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HLY87419.1"
                     /translation="TGGNLVQRTRCPYFYDTVTPCNKRQPGSGCSAIGGHNRMNAILG
                     TSEACIAVHPSDMCVALAALDATVHVAGHVAGQNDSRAIAFGDFHRLPGATPHIETNL
                     QRNEIIVAVELPAQGFATNYSYLKIRDRLSYAFALVSVAAALQLDGGRIREARLALGG
                     VAHKPWRDTSAEAALVGQAPDRAAFARAADMVLRDAKGLEHNAFKIDLAHRAIVRTLT
                     QAASGRPQSQSNKTIQ"
     gene            738..2960
                     /locus_tag="VKQ27_00410"
     CDS             738..2960
                     /locus_tag="VKQ27_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY87420.1"
                     /translation="MTAYIGTAVSRVDGRAKVTGTAKYAGEFSDASLAFGAVVTSRIA
                     KGRLKHIDTHEALAVDGVLAVLTHLDRPHMASEDSAYKDDVAPEVGSPFRPLYDDKIL
                     FSGQPIAVVIAEESEIARFAASLVHVAYEEAAHITDIFSRREGDVVVEPGSNMFAPPP
                     SRGTPDQALAAAKLRHHGEYYVPIEHHNPMEPYASTAIYEADGSLTIHDKTQGVQNVQ
                     RYMTHVLEMDPSQVRVLSPFVGGAFGSGLRPQYQVVLAALGALALKRSVRVVLTRAQI
                     YALGFRPAVIHKIGLGASTEGALDTVSHEAITITSRNEDFHRQETAWSGLLYTSAHAK
                     YAHSMTRLDLPTPCDMRAPSAAPSVYALESAMDELAIALKVDPVELRLRCYSDRDQHS
                     GHPYSSKALRDCYQRGATAFGWEKRTAEPRSMRDGSELIGWGMASGVWDAWQVPITVR
                     IVLTANGHAEVSCATSDIGTGTYTIMAQVAAEMLGLPLESVTIRLGDSTLPQSPVEGG
                     SWMAASVANGIAHTAGFIRDELLRMAKQMPNSPLAGTESTDVVLVDGALASRRDSARS
                     VSIADAMRHGAVDRIDLEKTVTFEDDGSCAHNTHSAVFAEVKVDEEIGVVRVTRVVSA
                     VAAGRILNTKTASSQILGGVVWGIGMALHEQTLIDHRFGRIMNADFAEYHVPVHADVH
                     DIKVIFVDEPDEQINPMGIKGLGEIGIVGVAAAIANAVYHATGVRVRDLPITLDKLHR
                     "
     gene            complement(3063..4382)
                     /locus_tag="VKQ27_00415"
     CDS             complement(3063..4382)
                     /locus_tag="VKQ27_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY87421.1"
                     /translation="MKDAQGHTLTGATAESAELFDLALRAFNLSCGDPVALLDAACEA
                     APDFTMAHIAKAWLFILARDPVLAERIDALIDTVAALAMNERERTHLAALQQAANHAL
                     SAAVLILDRHLMQYPFDFIAQPVALFLDLYQGRVRWMRDRTARALPRWSKDLPGYGSM
                     LAYHGFGLEECGDYDRAEDESRAAAELEPASQFAHHTVAHVMEMTGRPEDGLGWMLAR
                     EALWSTPEHLNQAHIWWHRALFHVELGQYSAALALYDGPIHATQRPIALSMTNALALL
                     WRLTMLGCDVGGRWAEVSSLWQGHADGRTLVFHDMHAVLAELGGGQPALVEHRLAAMR
                     ETAAGNDEAATLYREVGVPLTEGLMAFWCGRHADAVALLQPVRFQLWRIGGSHAQRDV
                     VDWTLTEAALRGRMRDVALSLAHERLGARPRSVVNRQFLQRAEALQA"
     gene            4463..5383
                     /locus_tag="VKQ27_00420"
     CDS             4463..5383
                     /locus_tag="VKQ27_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87422.1"
                     /translation="MLPTPGFHHLHLNSVDPDAAIDWYVRQFPSGSKGKWAGIPALLA
                     PNNVMVLFSKVATPPVSQPQSAIWHFGWHVKDARKTLATFQARDEVTLLPLYTSDEGG
                     SVLISSDTWPNVGNLLGLTQAQIAEARAAGAKPKGGGGFVYFTGPDDALAEFAGDYPA
                     ERFNHVHLWQEHPLCAQLWYQKHLNAPARASFDEVPVTAADCQVPRGADLTWPALKRE
                     GMYRTPRAGVEFGDVVLTWYANQGEAPLVSPRGQLQDHFALSVDDLDAWVTKLRGEGV
                     VVLEEPYRLGETRAVMIEGPSKEALELVEV"
     gene            5471..>6179
                     /locus_tag="VKQ27_00425"
     CDS             5471..>6179
                     /locus_tag="VKQ27_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87423.1"
                     /translation="MATQKVLILGASYGSLLASKLLFAGHEVTLICLPNEVEAINSDG
                     IRVKMPIRGRSAPVEIHSKALPGKVRAAGPAGINPADYDLVALAMQEPQYRSAGVREL
                     LDAVAKSRKPTMSIMNMPPLPYLKRIPGLNTDSLHDCFTDASVWANFEPGCLTLCSPD
                     PQAIRMPDDNINVLTVTLPTNFKAARFDSDASTNIMRQFQREIEAIRFNTGDAQIDLP
                     VKLKVHDSIFVPLAKWAM"
BASE COUNT         1166 a         1820 c         2070 g         1123 t
ORIGIN      
        1 gacgggcggg aatctggtgc aacgcacgcg gtgcccgtac ttctacgaca ccgtgacgcc
       61 ttgcaacaag cgccagcccg gcagcggttg ttctgccatc ggtggccata accgcatgaa
      121 cgcgatcctt ggcacaagcg aggcgtgcat tgcggtgcat ccgtccgata tgtgcgttgc
      181 gttggcagca ctggatgcca cagtacatgt ggctggacat gtggctggac agaatgactc
      241 gcgtgcgatc gcgtttggcg acttccatcg tttgcccggc gctacgccgc acatcgagac
      301 caatctgcag cggaacgaga tcatcgtggc ggtggagcta ccggcgcagg gatttgccac
      361 gaattacagc tacctgaaga tccgcgaccg tctttcgtat gcgttcgcgc tggtatccgt
      421 tgcggcggca ttgcagctgg acggcggcag gatcagggag gctcggctgg cgctgggtgg
      481 cgtcgcgcac aagccgtggc gcgacacatc ggctgaggcg gcgctggtgg gacaggcccc
      541 cgaccgcgca gcattcgctc gtgcggcgga tatggtgctg cgcgacgcga aaggcctcga
      601 gcataatgcg ttcaagatcg atctggcgca ccgcgccatt gttcgcacat tgacgcaggc
      661 ggcgtcgggc agaccgcagt cgcagtccaa taagaccatc cagtgatatc ggctcattcg
      721 ataggagctt acccagcatg accgcttata tcgggactgc cgtttcccgg gtcgacggcc
      781 gtgccaaagt caccggtacg gcaaaatatg ccggcgaatt cagcgatgca tcgcttgcgt
      841 tcggcgccgt cgtgacatca cggattgcca aaggccgcct caaacacatc gacacgcacg
      901 aggcgctggc tgtcgatggg gtgcttgcgg tgctgacgca tctcgatcgg ccgcacatgg
      961 cgagcgagga cagcgcctat aaggacgacg tggcgccaga ggtcgggtcg ccgttccggc
     1021 cactatatga tgacaagatc ctgttcagcg gccagccaat cgcggtggtg atcgccgaag
     1081 agtcggaaat cgcacggttt gcagcgtcgc ttgtgcacgt ggcctacgag gaggccgcgc
     1141 atatcaccga catattcagc cggcgcgagg gagatgttgt agtagagccg gggagcaaca
     1201 tgtttgctcc gccgccttcg cgaggcacgc cggatcaggc gctcgccgcg gccaagctcc
     1261 gccaccatgg tgaatactac gtgccgatcg agcaccataa tccgatggag ccatatgcat
     1321 cgaccgcgat ctatgaagcg gacggttcgc tgacgatcca tgacaagacg caaggcgtgc
     1381 agaacgtcca gcgttacatg acgcatgtgc tcgagatgga cccaagccag gtgcgcgtgc
     1441 tgtcgccgtt cgttggcgga gccttcggct cggggctgcg gccgcagtat caggtggtac
     1501 tggcggcgct gggggctctc gccctgaagc ggtcggtgcg cgtggtgctg acacgcgcgc
     1561 agatttatgc gctgggtttt cggccggctg tgatccacaa gatcgggctt ggcgccagca
     1621 cagagggagc gctggatacc gtctctcatg aagcgatcac catcacgtcg cggaacgagg
     1681 actttcatcg gcaggagact gcctggtccg ggctgctcta tacgtccgcc cacgcgaagt
     1741 acgcgcacag catgacccgg ctggatctgc ccacaccgtg cgacatgcgc gcaccaagcg
     1801 cggcaccctc ggtctacgcg ctggaatccg ccatggatga gctggcgatt gctctcaagg
     1861 tcgatcctgt ggaactgcgc ttacgctgct attcggatcg cgaccagcac agcggccatc
     1921 cgtacagcag caaggctctg cgcgactgtt atcagcgggg cgcgaccgcg ttcggctggg
     1981 agaagcgcac agccgaaccg cgttccatgc gcgacggcag cgagctcatc ggctggggca
     2041 tggcaagtgg cgtgtgggat gcctggcagg taccgatcac cgtgcggatc gtgctgacgg
     2101 cgaatggcca tgcggaagtt tcgtgtgcaa cttccgatat tggcacaggc acgtacacga
     2161 tcatggcgca ggtcgccgcc gagatgctgg ggctgccgct ggagagcgtg accattcggc
     2221 tgggcgattc gacactgcca cagtcgccgg tggaaggcgg ttcctggatg gcggcctcgg
     2281 tagcgaacgg tatcgcgcac actgcaggct ttattcgcga cgaactgcta cggatggcga
     2341 aacagatgcc aaactcgcct ctggctggta cggagagcac agacgtcgtg ctggtggatg
     2401 gcgcactcgc aagccgccgt gattcagcgc gctcggtgtc gatcgccgat gcgatgaggc
     2461 atggcgcggt ggatcggatc gatcttgaga agaccgtcac gttcgaggat gacggttcgt
     2521 gtgcgcacaa cacccactct gctgtgtttg ccgaagtgaa ggtggacgag gagattggtg
     2581 ttgtccgtgt aacccgcgtg gtgagtgcgg tggctgccgg acggatcctg aacaccaaga
     2641 ccgcaagcag ccagatcctg ggcggtgttg tatggggcat cgggatggcg ctgcatgagc
     2701 agacattgat cgatcaccgg ttcggccgca tcatgaacgc cgatttcgcc gagtatcatg
     2761 tgccggtgca tgccgacgtg cacgacatca aggtgatttt cgtcgacgag cccgatgaac
     2821 agatcaaccc gatgggcatc aaaggcctcg gtgagatcgg cattgttggc gtggcggcgg
     2881 cgattgccaa cgccgtttac cacgcgaccg gcgtgcgggt gcgcgatctg ccgatcacgt
     2941 tggacaagct gcaccgatag gccctgtgta aaccaatgtc gttgcgagcg gagcgaagca
     3001 atctcgtcgg gattgcttca ctccgctcgc aatgaccgca acagggggga ctaccgcacc
     3061 cgtcaggcct gaagggcttc ggctcgttgc aggaattggc gattcaccac gctgcggggc
     3121 cgggcgccca ggcgttcgtg cgccagcgag agggcaacgt cgcgcatgcg gccgcgcagg
     3181 gcggcttcgg tgagtgtcca gtccaccaca tcacgttgcg cgtgactgcc gccgatacgc
     3241 cagagttgga aacgcactgg ttgcagcagc gcgacggcgt cggcgtggcg gccgcaccag
     3301 aatgccatca ggccttccgt cagagggaca ccgacttcgc ggtaaagggt ggccgcttca
     3361 tcgttgccgg cggcggtctc gcgcatcgcc gcgagccggt gttccactag cgcaggctgg
     3421 ccgccgccaa gctcggccag cacggcgtgc atgtcgtgga acacgagcgt tcggccgtcc
     3481 gcgtggcctt gccacagcga tgacacttcc gcccaccgtc caccgacatc gcagccgagc
     3541 atcgtcagcc gccacaatag cgccagcgca ttggtcatgc tcagcgcgat cggtcgttgc
     3601 gtcgcgtgga tgggaccgtc gtagagcgca agcgctgcgc tgtactggcc aagttccaca
     3661 tggaacagcg cgcgatgcca ccagatatgc gcctggttga gatgctcggg cgtggaccag
     3721 agagcctcgc gcgccagcat ccagccgagg ccgtcttcgg ggcggccggt catttccatc
     3781 acatgcgcaa ccgtgtggtg ggcaaactgg ctcgcgggtt ccagttcggc ggccgcgcgg
     3841 gattcgtcct cggcgcgatc gtagtcgccg cattcctcga ggccgaagcc gtgataggcg
     3901 agcatcgacc cgtaaccggg cagatccttc gaccatcgcg gcaatgcacg cgcggtacga
     3961 tcgcgcatcc agcggacacg gccttgatac agatcgagga acagcgcgac cggctgggcg
     4021 atgaagtcga acgggtactg cataagatgc cgatcgagga taagcacggc tgcgctcagc
     4081 gcatggttcg ctgcctgttg cagggcggcg agatgggtgc gttctcgctc gttcatggca
     4141 agcgcagcaa cggtgtcgat gagcgcatcg atgcgctcgg cgaggacagg atcgcgggcc
     4201 aggatgaaca gccaggcttt cgcaatatga gccatcgtga aatcgggtgc ggcctcgcac
     4261 gccgcatcga gcagtgcgac gggatcgccg caggacagat tgaacgcacg cagcgcaaga
     4321 tcgaacagct cagccgattc cgctgtggca cctgtcagag tatggccttg cgcgtccttc
     4381 atcggttgcc tccctgtcca cgcgagcgtg gacgcatcgc ggcaacggtg caatcgtagc
     4441 gtgaacggcg ggagggacgc gcatgctgcc gacacctggg ttccaccatc tgcatctgaa
     4501 ctcggtcgat cctgatgcgg cgatcgactg gtacgttcgg cagtttccaa gcggctcgaa
     4561 gggcaaatgg gcaggaattc ccgcgttgct ggcgcctaac aatgtgatgg tgctgttcag
     4621 taaggtggcc acaccgccgg tgagccagcc gcagagcgct atctggcatt tcggctggca
     4681 cgtgaaggac gctcgcaaga cgctggcgac gttccaggcg cgggacgagg tgacgttgct
     4741 gccgctgtat acatccgatg agggtgggtc ggtactgatc agcagcgaca cctggccgaa
     4801 tgtcggcaac ttgctgggtc tgacgcaggc ccagattgcc gaagcgcgcg cggccggggc
     4861 gaagccaaag ggcggtggtg ggtttgtcta tttcactggg ccggatgatg cgctggccga
     4921 gttcgcaggc gattatccgg ctgagcggtt caaccacgtg catctgtggc aggagcatcc
     4981 gttatgcgcg cagctttggt accagaagca cctgaacgcg ccagcccggg cgagctttga
     5041 cgaggtgcca gtgaccgcgg ctgactgtca agtgccgcgc ggcgcggatc tgacgtggcc
     5101 ggcgctgaaa cgcgagggca tgtatcggac gcctcgagcc ggcgttgagt tcggcgacgt
     5161 ggtgctgacc tggtacgcga accagggcga ggcgccgctg gtgagtcctc ggggccaatt
     5221 gcaggatcat ttcgcgctaa gcgttgacga cttggatgcg tgggtgacga agctgcgcgg
     5281 cgaaggtgtg gtcgtgttgg aggaaccata ccggctcggc gaaactcgtg ccgtgatgat
     5341 cgagggtccg agcaaggagg cgctggaact ggtcgaagtc tgacccccca gattcccatt
     5401 ttctcctggc tgtgccacac ttaccggcag ggcgggcgca aagcaccgtc tgctagggag
     5461 tgaatgttca gtggccacac aaaaagtgct cattctcggc gcgtcctatg gatcgctgct
     5521 tgcaagtaaa ctgctcttcg ccggacacga agtcacgctg atctgcctgc ccaacgaagt
     5581 tgaggcgatc aacagcgacg gaatccgcgt gaagatgcca atacgcggtc gtagcgcgcc
     5641 ggttgaaatc cactcgaagg cactccccgg caaggtaagg gccgcggggc cggctgggat
     5701 caatccagcc gactatgatc tggtggcact ggcgatgcag gagccgcagt accgttcggc
     5761 gggcgtgcgg gagttgctgg atgcggtggc gaagtcgcgc aagccgacga tgtcgatcat
     5821 gaacatgcct cccctgccct acctgaagcg cattccgggg ctgaataccg acagcctgca
     5881 cgactgcttc acggatgcga gcgtatgggc gaatttcgag cctggctgcc tcacgctttg
     5941 cagccccgat ccgcaagcga tccgcatgcc ggatgacaac atcaacgtgt tgacggtgac
     6001 gctgccgacg aacttcaaag cggcccggtt cgattcggat gcgtctacca acatcatgcg
     6061 gcagttccag cgggagatcg aggcgatccg cttcaacaca ggcgacgccc agatcgattt
     6121 gccggtgaag ctgaaggtgc atgactcgat cttcgtgccg cttgcgaaat gggcaatgc
//
LOCUS       DATGEM010000018         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1248445, whole genome shotgun sequence.
ACCESSION   DATGEM010000018 DATGEM010000000
VERSION     DATGEM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248445"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VKQ27_00430"
     CDS             <1..192
                     /locus_tag="VKQ27_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87424.1"
                     /translation="IRQHKALGQPEAGLVEAPLQARLASGCRRGMACALTGSVTDRHS
                     DKINNFAVAGATCEGVDEN"
     gene            complement(189..845)
                     /locus_tag="VKQ27_00435"
     CDS             complement(189..845)
                     /locus_tag="VKQ27_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87425.1"
                     /translation="MDLAERKPEGWTLIGAQMVALHGMEHGKLPPRSSEDLDILVNIR
                     LITHGTEEFSRLLLEIGMDLDGSSPTGLAHRFVGRGTKVDILAPDGAGERAHLTTIPP
                     GQTVMVPGGTKALAHTERVQVRLGDRIGEIPRPDMLGALVIKSCAVDIDDAPENQRRD
                     LVFLLSLVGDPRVTAAMLDTKERAILRRRSDLLDRNAPAWRSIDNADDAHLALRILAG
                     "
     gene            complement(896..1630)
                     /locus_tag="VKQ27_00440"
     CDS             complement(896..1630)
                     /locus_tag="VKQ27_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY87426.1"
                     /translation="MAITTDMLTANEAARRLGVTQKRVYSLVASGALSARRLGNRFLL
                     DPSEVEARKMQGIVTGRPLDQTSAWAVLWLASARDKRDLGPWGAYLARNTRWRVRQKL
                     GEGPLAAELAALAPRLRRRGRLVHMRAHPSDLPRLLDEPDVVRTGISAAADVGADIVA
                     PADLEAYAPAPRFDELRGRYFLEESNAPNVWLRLIHEPWPFPSGTRVAPAAAVALDLL
                     DSADERTRRAGRELLDAIDQYGRDPT"
     gene            complement(2385..>2676)
                     /locus_tag="VKQ27_00445"
     CDS             complement(2385..>2676)
                     /locus_tag="VKQ27_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87427.1"
                     /translation="LLGFVGDRPRLYPRWTPIFVCITWMLDLDDRAGEFAKQVAMVEA
                     TRVFREISGFVDAANLVKPETPIVGRDALHTFLAWAEELTGRLAAGDEAVFR"
BASE COUNT          522 a          817 c          820 g          517 t
ORIGIN      
        1 attcgacagc ataaggccct cggacagccc gaagcggggc ttgttgaagc gccacttcag
       61 gcgcgactag caagtggctg tcgacgcgga atggcttgtg ctttaactgg ttctgtcacc
      121 gatcgacact cagacaaaat caataatttc gctgttgccg gcgctacctg cgaaggtgtc
      181 gacgagaatt agccggccag gatgcgcagt gctaggtggg catcatctgc attatcgata
      241 ctccgccatg ccggcgcatt gcggtccagc aggtcggagc gcctccgaag aatcgcacgt
      301 tccttcgtgt caagcatagc ggccgtaaca cgcggatcgc cgaccaggct caacaagaac
      361 acgaggtctc ggcgctgatt ctcaggcgcg tcgtcaatgt cgacggcgca cgacttgatg
      421 actaacgctc cgagcatgtc tggtcgtggg atttctccga tgcggtcgcc taaccgaacc
      481 tggacccgtt cggtgtgtgc aagagccttg gtcccgccgg gaaccatgac tgtttggccg
      541 ggcggaatgg tcgtcagatg tgcgcgttct cccgccccat caggtgcaag aatgtcaacc
      601 ttcgttccac ggcctacgaa acgatgggcg agaccggttg gactcgagcc atcgagatcc
      661 atgccgattt cgagcagcag gcgactgaac tcctcggtcc cgtgagttat caagcggata
      721 ttcaccagaa tgtcgagatc ctcgcttgac cttggcggca gcttcccgtg ctccatgcca
      781 tgcagcgcca ccatctgggc gccgatgagc gtccaacctt cgggcttacg ctccgccagg
      841 tccatggcaa gattccaagt actgtgcagc gttggaggag cttgacccaa gtcgatcacg
      901 tcggatcacg gccgtattgg tcgatggcat cgaggagctc ccgaccggcc cgccgcgtac
      961 gttcgtccgc tgagtcgagc aggtcgagtg ctacagcagc tgcgggtgcg acgcgcgtgc
     1021 cagagggaaa cggccatggc tcgtggatca ggcgtaacca aacgttgggc gcgttacttt
     1081 cctcgaggaa atagcggcct cgcagttcat cgaagcgtgg tgcgggagcg taggcttcca
     1141 aatcggcggg cgccacgatg tcggcgccga catcagctgc ggcacttatc cctgtccgca
     1201 ccacgtccgg ctcgtccagc agccgtggta agtcgctcgg gtgcgcacgc atgtgcacaa
     1261 gacgaccacg gcgtctgagc cgcggcgcga gcgccgccaa ttcagctgcc agcggacctt
     1321 cgcccaactt ctggcgaacg cgccatcgcg tgtttctggc tagataagct ccccatggtc
     1381 cgagatcgcg cttgtcacga gccgaggcaa gccacaggac ggcccaggca ctcgtctggt
     1441 cgagcggacg accggtgaca atgccctgca ttttgcgcgc ctcaacttcc gatggatcga
     1501 gcaggaagcg attcccgagg cgacgagcgg acagagctcc cgatgccacg agcgaataca
     1561 cgcgtttctg ggttacccca agccggcgcg ccgcctcgtt agccgtcaac atgtcggtcg
     1621 tgattgccat gcaccagaac tatagacgat cgtcgctagt tgtggggttc ggtctgattg
     1681 gcgttcttgg cccccaccgt caactgtgtg cgtgctgcgg tgcgccagaa ctatagacaa
     1741 tcatcactaa ttgtgaggat tgaagtgcga tagttggtct caactggggt agccgcccgg
     1801 ccggaatcgt ggcgattctt gacgagctct ggcgaacgat tgggtgaaat tttgaacgtg
     1861 agcgcgaacg attctaatct tcaaatggcg gctaccgggg tcagaaggcc caacgcttaa
     1921 tcgcttggtt ctcggttcga gtcggaggga ctcacttccc agcccatggc agcaccgaaa
     1981 tcgtctccgc cgatatccgc ccgagatgcg cgaacgcgcc gtccgcatgg tccaagagac
     2041 aattgccgaa agcggcaagc gccagggcag cgtgcgcaat tacctggcgc gtgccaagcg
     2101 ggccggtctg agctggcaat tacccgagca gcttgacggc gcggttgcgc atgtgttcgt
     2161 cagcgtgttg ggcgcgagcg gctacctgtg cgcggaagcc atgctcgggt cgcccacggc
     2221 cgagtcgagc tcttcattcg gacctcgtgg ccttcgccac ccgccgcctg actccaattc
     2281 gatcggtgta cgtccgcgac ggttgctacg atcgctgcga gcgcccggcg aagctcgcga
     2341 ttggcaccaa agagacacgg cttcgggatc ccaccgcgtc tgaactaccg aaagacggct
     2401 tcgtcaccgg cggcaagtcg gccagtgagc tcttcggccc aggccaagaa tgtatgcaac
     2461 gcgtcgcgcc ccacaattgg cgtttcaggt ttcacgaggt tcgccgcgtc aacaaatccg
     2521 ctgatctcgc ggaaaacacg ggtggcttcg accatcgcca cttgcttcgc gaactcgccg
     2581 gctcggtcgt cgagatcgag catccacgtg atacagacga agatcggagt ccatcgcggg
     2641 tagagccttg ggcggtcgcc gacaaaaccg agcagg
//
LOCUS       DATGEM010000019         4425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1707397, whole genome shotgun sequence.
ACCESSION   DATGEM010000019 DATGEM010000000
VERSION     DATGEM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707397"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VKQ27_00450"
     CDS             <1..1155
                     /locus_tag="VKQ27_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87428.1"
                     /translation="FAGLFVGTIAFSYFADRFGRRAIFTFSLVWYSIATLILSFQNSA
                     DMVNLWRFIGGIGIGVELVTIDTYISELVPKRVRGRAFAYQQGIGFCAVPLIALLAWL
                     LVPTDPLGISGWRWVVVIGSVGALFIWWLRLGLPESPRWLAQQGRFEDAERVMQAIEA
                     KVASETGQALPPPELAPPENPRAGSYWEVFSPAYRGRAIMMIVANFCSTIGFYGFANW
                     VPTLLIAKGIHVTQSLQYTFIMAFAYPLFALFSTTFADRMERKWQVVAASIGMAVCGV
                     IFSMQDAAAALIALGFIQTMMNTWISFSFHNYQAELFPTRMRARAVGFVYSWSRFSTI
                     FTGFFIAFFLKNFGVEGVFGFIAVAMAIVAISIGGFGPRTNHRPLEEISH"
     gene            complement(1170..2342)
                     /gene="rmuC"
                     /locus_tag="VKQ27_00455"
     CDS             complement(1170..2342)
                     /gene="rmuC"
                     /locus_tag="VKQ27_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HLY87429.1"
                     /translation="MVELLAGAAMLAAIAALLVVSRQASSFERLRLLSEQSLANTRAE
                     AETTRAALSREQGQQRQEITLHFDRARAMLEGKLRELREGNETKLAEIQKTVNEQLHA
                     AVEKQMNESFNRVIDQFTAVQKAMGDVQAVTAQIGDIKRLFSNVKTRGGWGETQVRAM
                     LDDILPPGEYEANCKVRADSNDVVEFAVRMPMKGEDRPLLPIDAKFPVEDYERLLAAS
                     DAGDAEAERTASRMLERRMRDEARKIAAKYINPPVTVEFAVLYLPTDALYTEVARIPG
                     LIDDIGRDCRVLVMGPSLFPALLRTIHLGFITLALEQKADQIRDLLGATRTEMLKMDE
                     VLDRLAKQAGSFSNTIESARRRTRAVSRKLRGVEAIETERAEQLLELDVDAELTED"
     gene            2488..3339
                     /gene="ehuB"
                     /locus_tag="VKQ27_00460"
     CDS             2488..3339
                     /gene="ehuB"
                     /locus_tag="VKQ27_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033294 - ectoine binding [Evidence IEA];
                     GO_function: GO:0033295 - hydroxyectoine binding [Evidence
                     IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="HLY87430.1"
                     /translation="MRRCLGILCLVVSMLALTGQNAGAQSTLERAKKDGIIRMGFANE
                     APWSFAQTDGTIVGADHDLAVLVFSRLGIPKIEGVLTKFGSLIPGLKADRFDVIVAGL
                     YIRPERCRQVAFSEPTAAVGDAVVVLAGNPKKITGFKSVVDDPSAKLGGVVGAATAQN
                     ARTAGVPDSQITMFPDFVSAVVALKAGRVDGALQTAITAQTTVDADPALARASPFQQA
                     IINGKPAVNYAAFAFRPEDHDLVQVFNAELAKILGTPEYLAMVAKYGLTANEVPKGVT
                     TEQLCGQ"
     gene            3364..4032
                     /gene="ehuC"
                     /locus_tag="VKQ27_00465"
     CDS             3364..4032
                     /gene="ehuC"
                     /locus_tag="VKQ27_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="HLY87431.1"
                     /translation="MSPEAVTGVVSLILAGTAVTLRIFLLSAMIAGPLAFLLGVLRTA
                     QSARVRVLTTMWVEFFRGTSTVVQLFWLYFALPFLGVSLSAQQAAVLGLGLVHGAYAS
                     EVVRGAILAVPRNQWETAQALALPRLATLRKVILPQALPSMLPPLGNAAIFLLKGTSI
                     ASIITVPELTFQSSLIVTRTMAIFQVMGCTLVIYYVLALLLVRLVRMLEARSGAWRPA
                     RQTP"
     gene            4029..>4425
                     /locus_tag="VKQ27_00470"
     CDS             4029..>4425
                     /locus_tag="VKQ27_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY87432.1"
                     /translation="MTTRFDWALTIEIMPKLLRAAEITVSITVLAYLLCLVGGGLLLA
                     LRRARTHSIARCAEWFVDFVRSTPLLPQLYFFYFALPSLHIDPSPYVIGVVAVGLHYS
                     CYMSEVYRAGLEAVPKGQWEAAEALGLSRF"
BASE COUNT          700 a         1321 c         1466 g          938 t
ORIGIN      
        1 ttcgccgggc tgttcgtcgg caccatcgcg ttctcgtact ttgccgaccg gttcgggcgg
       61 cgtgcgatat tcacgttctc actggtctgg tacagcatcg cgacgttgat cctgtcgttt
      121 cagaattcgg ccgacatggt gaacctatgg cggttcatcg gtggcatcgg catcggcgtg
      181 gagctggtga cgatcgatac ctacatctcc gagcttgtgc cgaagcgcgt gcgcggccgg
      241 gccttcgcct atcagcaggg catcggcttc tgcgcggtac cactgatcgc gctgcttgca
      301 tggctgctgg tgccgacgga tccgttgggc atatccggct ggcgatgggt cgtcgtgatc
      361 gggtcagtcg gcgcgttgtt catctggtgg ttgcggcttg ggctaccgga atcgccgcgc
      421 tggctggcac agcaggggcg gttcgaggac gctgaacgcg tgatgcaggc gattgaggca
      481 aaggtggcct cggaaaccgg tcaggcactg ccaccgcccg aactggcacc acccgaaaat
      541 ccgcgcgccg gttcctattg ggaggtgttc agtccggcgt atcgcggtcg tgccatcatg
      601 atgatcgtcg cgaacttctg ctcgacgatc gggttctatg gcttcgccaa ctgggtgccg
      661 acactgctga tcgccaaggg catccatgtg acgcagagcc tgcaatatac cttcatcatg
      721 gcgttcgcat atccattgtt cgcgctgttc agcacgacgt tcgccgaccg gatggagcgg
      781 aagtggcagg tggttgccgc atccatcggc atggcggtgt gcggcgtgat cttctcgatg
      841 caggacgcgg cggcggcatt gatcgccctg ggcttcatcc agacgatgat gaacacctgg
      901 atctcgttct cgttccataa ctaccaggcg gaattgttcc cgacgcggat gcgtgcacgc
      961 gctgttggct tcgtctattc atggagccgg ttcagcacga tcttcaccgg gttcttcatt
     1021 gcgttcttcc tgaagaattt cggtgtggag ggtgtgttcg ggttcatcgc ggtggcgatg
     1081 gcgattgtcg cgatctccat cggcgggttt gggccacgca cgaatcaccg gccgctggag
     1141 gagatttcgc actgagcagg ctgcggctgt cagtcctctg tcagctccgc atcgacgtcc
     1201 agctccagta actgttcggc gcgttccgtc tcgatcgcct cgacgccgcg cagcttgcgg
     1261 ctgacggcac gggtgcggcg acgggcagac tcgatcgtgt tgctgaagct gccggcctgt
     1321 ttcgcgaggc gatccagcac ctcgtccatc ttcagcatct cggtgcgggt ggcaccgagc
     1381 aggtcgcgga tctgatcggc cttctgctcc agcgcgagtg tgatgaagcc gaggtggatg
     1441 gtgcgaagca gggctgggaa caggctgggt cccatcacca gcacacggca gtcacggccg
     1501 atgtcatcga tcaggccggg gatgcgcgcg acctcggtgt aaagcgcatc ggtgggcaga
     1561 tagagcacgg cgaactccac cgtcaccggc gggttgatgt atttcgcggc gatcttgcgg
     1621 gcttcgtcgc gcatgcgccg ctccagcatg cgtgacgcgg tgcgttcagc ttctgcatca
     1681 ccggcatccg aagcggcaag gaggcgctcg tagtcctcga ccgggaactt cgcgtcgatc
     1741 ggcagcagag ggcggtcctc gcccttcatc ggcatgcgga cggcgaattc caccacgtcg
     1801 ttgctgtcgg cgcggacctt gcaattcgcc tcgtactcgc cgggcggcaa gatgtcgtcg
     1861 agcatcgcgc gcacctgggt ttcgccccag ccgccgcgcg tcttcacgtt tgaaaacagg
     1921 cgcttgatgt cgccgatctg cgcggtgaca gcctgcacgt cgcccatcgc cttctgcacc
     1981 gcggtgaact ggtcgatcac gcggttgaag ctttcgttca tctgcttctc gaccgcggcg
     2041 tgaagctgct cgttcaccgt tttctggatt tccgccagct tcgtttcatt gccttcacgc
     2101 agctcgcgca gcttgccttc aagcatggcg cgtgcgcgat cgaaatggag ggtgatctcc
     2161 tggcgctgct ggccttgctc gcgtgagagc gcggcgcggg tggtttccgc ctcggcgcgg
     2221 gtgttggcga gcgactgttc ggagagcagg cgcaggcgtt cgaacgacga cgcctgccgc
     2281 gacaccacga gcagtgcggc gatggcggcg agcatggcgg cgccggccag cagttcgaca
     2341 atcatgacgc acacctgaag ctatcgggcc ggtagcttag caccgccggc gattcgcggg
     2401 cggcccgcac ggcctgatcg aaaatacgca tgtcccgctg caaagtgcca tgctaccgtt
     2461 cgcgggatat ccacggggag gcgaaagatg cggcgatgcc tcggcatcct gtgcctggtc
     2521 gtatcgatgc ttgcgctgac cgggcaaaac gcgggcgcgc agtccacgct ggagcgggcg
     2581 aaaaaagacg gcattatccg catgggattc gccaatgagg ctccctggag cttcgcacag
     2641 actgacggca cgatcgtcgg ggccgatcat gatctcgccg tgctggtatt cagtcgcctc
     2701 ggcattccca agatagaggg ggttctcacc aagttcggat cgctgattcc gggactgaag
     2761 gcggatcgct tcgacgtgat cgtcgccggg ttgtatatac gacccgagcg gtgcaggcag
     2821 gtggccttca gcgagccgac ggcagcggtt ggcgatgcgg tggtggtgct ggccggtaac
     2881 ccgaagaaga ttaccggctt caaatcggtg gtggacgatc cctcggcaaa gcttggcggc
     2941 gtggttggcg cggcgacggc acagaacgcg cgcacagctg gcgtgccgga cagccagatc
     3001 accatgtttc cggacttcgt ttccgcggtt gtcgcgctga aggccgggcg ggtcgatggc
     3061 gcactgcaga ctgcgataac cgcgcagacg acggttgatg cggatccggc gttggcgcgt
     3121 gctagcccct tccagcaggc gatcatcaac ggcaagccgg cggtgaatta tgcggcattc
     3181 gccttccggc cggaggatca tgatctggtg caggtcttca acgccgaact cgcgaagata
     3241 ctgggcacac ctgagtatct tgccatggtc gcgaaatacg ggctgacggc caacgaagtt
     3301 cccaaaggcg tcacaacgga gcagctttgc ggccagtgat ttcgaccgcc gcccaggttt
     3361 gcgttgtcgc ctgaggcggt gacgggcgtc gtatcgctga tactggcggg caccgccgtc
     3421 acgctgcgaa tttttctgct atcggcgatg atcgccggac cactggcctt tctgctgggt
     3481 gtgctgcgca ctgcgcagtc cgcccgcgtg cgcgtattga cgacgatgtg ggtcgagttc
     3541 tttcgtggca cctcgacagt cgtgcaactg ttctggctct atttcgcgct gccgttcctg
     3601 ggcgtatcgc tgtcggcgca gcaggcggcc gtgctcggtc tcggcctggt gcatggtgcg
     3661 tacgcgtccg aggtggtgcg tggggcgatc ctcgccgtcc cgcgcaacca gtgggagact
     3721 gcacaggcat tggcgctgcc gcgattggct acgctgcgaa aggtcatact gccccaggcg
     3781 ctgccatcga tgctgccgcc tttggggaac gcagcgatct tcctgctgaa gggcacgtcc
     3841 atcgcatcga tcatcaccgt gccagagctg acctttcagt cgtcactgat cgttacgcgc
     3901 accatggcga ttttccaggt catgggctgc accttggtca tctactacgt gctcgcgctg
     3961 ttgctggtgc gactggtgcg gatgttagag gcccggtcgg gagcgtggcg gccagcccgg
     4021 caaacgccat gacaacgcgg ttcgactggg cactcaccat cgagatcatg cccaaattgc
     4081 tgcgggccgc ggagatcacg gtctcgatca ctgtgctggc ctatctcctg tgtctggtgg
     4141 gtggcggctt gctgctggca ctgcgccgag cacggacgca ttccatcgcg cgatgcgccg
     4201 agtggttcgt ggatttcgtg cgcagcacgc cgctgctgcc ccagctgtat tttttctatt
     4261 tcgccttacc gtcattgcac attgatccga gcccatacgt catcggcgtg gtggcggttg
     4321 gcctgcacta tagttgctac atgagcgagg tgtaccgggc gggacttgag gccgtcccca
     4381 aggggcagtg ggaagccgcc gaggcgcttg ggctgtcccg gtttc
//
LOCUS       DATGEM010000020         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_675038, whole genome shotgun sequence.
ACCESSION   DATGEM010000020 DATGEM010000000
VERSION     DATGEM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675038"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VKQ27_00475"
     CDS             <1..1615
                     /locus_tag="VKQ27_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLY87433.1"
                     /translation="VMFTVTMALGSKELAQRGVLVTRLSAAEDAATMSVLCVDKTGTI
                     TLNQLAVMDVIPLGDSTDTEVLIAGALASQEANQDPIDLAFLAAAKARNVFDEFAPVV
                     PVSFAPFDAMNRRTEAVVQQGGRRLRFMKGAVRTIAAACGQQPGAMDLLEQRVRESAA
                     KGYRTLAVARGAETGTPILLGLVSLNDPPRPDAKRLIADLQNLGVAVKMVTGDALAVA
                     QDTGRNLGLPNIRRASELKARRLEAGAAQADFLAGVDGFAEIYPEDKFVVVKQLQALG
                     RVTGMTGDGVNDAPALRQAEVGIAVSVATDVAKGAASVVLTEPGLTNIVTLIDQGRSI
                     YQRILTWIINKISRTILKAGFVAIAFVVTGKFVVSAFAMLLLVFMTDFAKIALATDHV
                     HPSRRPETWNIDGFVAVSVVLGVAMVAETLLLLWFSWSRYGLSVHDDALFTFSFLVLL
                     YFAVFSVISARERRGFWTTVPSKTLMAALVADALTGTVLTYLNIPGLRPLPLRQTGGI
                     FVYAIAACLGVNDAIKVAMIKWRVPTAIA"
     gene            complement(1720..2547)
                     /gene="dapD"
                     /locus_tag="VKQ27_00480"
     CDS             complement(1720..2547)
                     /gene="dapD"
                     /locus_tag="VKQ27_00480"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HLY87434.1"
                     /translation="MYDTLRATIEAAYERRAQITPQSTTAEVGNAVETIVDALDAGTL
                     RVAERRDGAWVVHEWLKKAVLLSFRIAPNVPQSAGALAFYDKVPLKYSGWDEPRFAAA
                     GVRVVPPATVRRGAYVAANVVLMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLS
                     GGVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVVVETGAVISMGVFIGQSTRIYYR
                     ERDEILYGRVPAGAVVVPGTLPAANGKYGLACAVIVKRVDAQTRAKTSLNDLLRNTD"
     gene            complement(2615..>4312)
                     /gene="glnD"
                     /locus_tag="VKQ27_00485"
     CDS             complement(2615..>4312)
                     /gene="glnD"
                     /locus_tag="VKQ27_00485"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HLY87435.1"
                     /translation="SLLNEMLLQLFREAILTQSAPPTPINPRFQIRNDYLEVTHPEVF
                     ARYPSALLEMFVLLQQHPELRGVRAETIRLIGTHVGLIDEEFRQNPRHHRLFIEILCA
                     PVGVTHELRRMNLYGVLGRYIPSFGRVVGRMQYDLFHAYTVDAHTLFVVSNLRRLAIP
                     KFNDEFPRLSQIMQSLPRQELAYLAALFHDIAKGRGGDHSELGAVDAEAFCLEQGLGR
                     YEARLVAWLVRNHLILSITSQKKDISDPDIIQAFARQVGDQVHLDYLYVLTVSDVRGT
                     NPKLWNAWKARLFEEFYERTKRALRRGLETPIDQDELIRETQAHAREKMTNIPDARIA
                     AVWSQWTEAYFLRYTPEEIAWQTSVLAGRDPSDESPFVAIRQLADRGGTVVLTYTPYR
                     LHSFARTTAVLDQMGLNVVDARLITSANGFSLETYVVLEDNGAVISDPERLEEIERGL
                     WRNLKQPEDSPLAVTRRAPRQVRMFSTPTQVNFSLDSRNDRTILELIAADRPGLLSEV
                     GKVFKAEQISINGAKIMTVGERAEDVFYITDSQDRTLGDDACRRVQDALVRALDRRN"
BASE COUNT          750 a         1314 c         1422 g          826 t
ORIGIN      
        1 tgtgatgttt acggtcacga tggcgttggg atcgaaggaa ctggcgcagc gtggggtgtt
       61 ggtcacccgc ctgagtgctg ccgaggatgc tgcgacgatg agcgttctgt gcgtcgacaa
      121 gaccggaacg atcacgttga atcagcttgc cgtcatggac gtaataccgc tcggcgactc
      181 gaccgacacc gaggtgctga ttgccggtgc gctggcgtcg caggaggcga atcaagatcc
      241 gatcgacctg gcgtttcttg cggccgcgaa ggccagaaac gtgttcgacg agttcgcgcc
      301 cgtcgtgccc gtctcattcg cgccctttga tgcgatgaac cgccggacgg aagccgtggt
      361 gcaacagggt ggccgtcgcc tgcgcttcat gaagggcgcc gttcgcacga tcgctgctgc
      421 ctgcggtcag cagccagggg cgatggatct tttggaacaa cgcgtcagag agtcagccgc
      481 gaaggggtat cggacgctgg ccgtcgcgcg gggtgccgag acaggcaccc caattttgct
      541 gggattggta tccctgaacg atccgccgcg gccggacgcc aagcgattga tcgccgatct
      601 gcagaacctc ggtgtcgccg tgaagatggt cacgggggac gctctagccg tggcgcagga
      661 taccggacgc aacctgggtt tgcccaacat ccgccgtgca tctgaactca aggcgcggcg
      721 cctggaagcg ggcgccgcgc aagcggattt tctggcggga gtcgatgggt tcgcggagat
      781 ctatccggaa gacaagttcg tcgtggtgaa gcagcttcag gcgctcggcc gggtcaccgg
      841 gatgacgggt gatggagtca atgatgcgcc cgcgctgcgc caggccgaag ttggcatcgc
      901 cgtgagcgtc gcgaccgatg tggcgaaggg cgccgcgagc gtggtcctga cggaaccggg
      961 gttgacgaat atcgttacct tgattgacca ggggcgaagc atttaccaac ggattctgac
     1021 ctggatcatc aacaagataa gccggaccat cttgaaagcg ggattcgtgg cgattgcatt
     1081 cgtggtgacc ggcaaattcg tggtgtccgc ctttgccatg ctgctgctgg tgttcatgac
     1141 ggacttcgca aagatcgccc tcgccaccga tcacgttcac ccgtccaggc ggccggaaac
     1201 ctggaatatt gacggcttcg tcgccgtttc cgtggtgctc ggtgtggcga tggtggcgga
     1261 gacgttgctt ctactttggt tcagctggtc acgctatgga ttatccgtcc atgacgatgc
     1321 tcttttcacc ttcagttttt tggtgttgct ctactttgcc gtgttctcgg tgatatccgc
     1381 gcgcgagcgc cggggattct ggacaaccgt accgagtaag acgctgatgg ccgccctggt
     1441 cgcagacgcg ttgaccggca cggtcctcac gtacctcaat atcccgggcc tcaggcccct
     1501 gccgttgcgg cagacaggtg gcatcttcgt ctacgccatc gcggcgtgtc tgggggtgaa
     1561 cgatgccatc aaggtcgcca tgatcaagtg gcgcgtcccc actgcgatag cgtagcttca
     1621 cagcttgatt ccataagcgg agatcagcgc tccgggaaca tccaacccag gcgagtgcga
     1681 tgcccgcggc gggcaaggcc gcggcacccg tcccgcgcct cagtcggtgt tgcgcagcaa
     1741 gtcgttcagc gaggtcttgg cgcgggtctg cgcgtccacg cgcttgacga tcacggcgca
     1801 ggccaggccg tatttcccat tggccgccgg caaggtgccg ggcacgacga ccgcgccggc
     1861 cggtacccgg ccatagagga tctcatcgcg ctcgcgatag tagatgcggg tgctctggcc
     1921 gatgaacacg cccatcgaga tcacggcacc ggtctcgacg accacgccct cgacgatttc
     1981 ggagcgggcg ccgatgaagc agttgtcctc gatgatggtg ggtgcagcct gcagcggctc
     2041 gaggacgccg ccgatgccga ctccgccgga caggtgtacg ttctttccga tctgcgcgca
     2101 ggagccgacg gtggcccagg tatcgaccat ggtgcccgag tccacatacg ctccgatgtt
     2161 cacatagctc ggcatcagca ccacgttggc cgcgacgtat gccccgcgcc ggaccgtggc
     2221 cggcggcacc acgcgcacgc cggccgccgc gaaccgcggc tcgtcccagc cgctgtattt
     2281 gaggggcacc ttgtcgtaga aagccagggc gccggccgac tgtggcacgt tcggggcgat
     2341 ccgaaacgac agcagcacgg ccttcttcag ccactcgtgg acgacccacg cgccatcgcg
     2401 gcgctcggcg acccgcaatg tccccgcgtc gagcgcgtcg acgatggtct cgacggcgtt
     2461 tcccacctcg gccgtggtgg attgcggcgt gatttgcgcc cgccgttcat aggcggcctc
     2521 gatggtcgcg cgtagcgtgt cgtacaattg ctggtctccg ggaatcactt gatggtcgat
     2581 cgaagattgc tgcgcaactt taggctccgg gcaatcaatt gcggcgatcg agggcgcgaa
     2641 ccaaggcgtc ctggacccgc cggcaggcat cgtcgcccag cgtccggtcc tgcgaatcgg
     2701 tgatgtagaa aacgtcctcg gcgcgctcgc ccaccgtcat gatcttcgcg ccgttgatgg
     2761 agatttgctc cgctttgaac accttcccca cctcggacaa aaggccggga cggtccgcgg
     2821 cgatcaattc gaggatcgtc cgatcattgc gggagtccag gctgaaattg acctgcgtcg
     2881 gcgtcgagaa catgcgcacc tggcgcggtg cgcggcgggt cacggcgagc ggcgaatcct
     2941 cgggttgctt gaggttgcgc cataggccgc gctcgatttc ttccaggcgc tcgggatcgc
     3001 tgatcaccgc gccgttgtcc tccagcacca cgtaggtttc gaggctgaag ccattggcgc
     3061 tggtaatgag gcgcgcatcg acgacgttga ggcccatctg gtcgagaacc gcggtggtgc
     3121 gggcgaagct gtgcaggcga tatggcgtat acgtgagaac gaccgttccg ccgcgatcgg
     3181 cgagctgccg gatcgcgacg aaaggcgatt cgtcgctcgg gtcgcggccg gcaagcacgc
     3241 tcgtctgcca ggcaatctcc tcgggcgtgt agcgcagaaa gtacgcttcg gtccattgcg
     3301 accacacggc cgcgattcgc gcgtccggta tgttcgtcat cttctcgcgc gcgtgcgcct
     3361 gcgtctcgcg gatcagctcg tcttggtcga tgggcgtttc gagcccccgg cgcaacgcgc
     3421 gtttggtgcg ttcgtagaac tcctcgaaca gccgcgcctt ccaggcattc caaagttttg
     3481 ggttcgtgcc gcgcacgtcc gagacagtca gcacatacag atagtccaag tggacctgat
     3541 cgccgacctg gcgcgcgaac gcttggatga tgtccgggtc gctgatgtcc tttttctgcg
     3601 aggtaatgga caggatgagg tgattgcgca ccagccaggc gaccagccgc gcttcatacc
     3661 ggccgagtcc ctgctccagg cagaaggcct cggcgtcgac ggcgcccaat tccgaatgat
     3721 caccgccgcg gcccttggcg atatcgtgaa aaagcgcagc caggtaggcg agctcctggc
     3781 gcggcagcga ctgcatgatc tggctcaacc tcgggaactc gtcgttgaac ttcggaatgg
     3841 ccaggcggcg cagattgctc acgacgaaca aggtgtgtgc atcgaccgtg taggcatgaa
     3901 acaggtcgta ctgcatgcgg cccaccacgc ggccgaaaga cgggatatag cggccgagca
     3961 caccgtagag gttcatgcgg cgcagttcgt gcgtcacgcc gacgggcgcg cacaggatct
     4021 cgatgaacag gcggtgatgg cgcggattct ggcggaactc ttcgtcgatc agccccacgt
     4081 gcgtcccgat cagccggatc gtttcggcgc gcacgccgcg cagttccgga tgctgctgca
     4141 gcagtacgaa catttccagc agagccgacg ggtagcgcgc gaacacctcg ggatgcgtca
     4201 cttccagata atcattgcgg atctggaagc gcggattgat gggcgtcggc ggcgcgctct
     4261 gcgtgaggat tgcctcgcgg aacagctgca gcagcatttc gttgagcagg ct
//
LOCUS       DATGEM010000021         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1361780, whole genome shotgun sequence.
ACCESSION   DATGEM010000021 DATGEM010000000
VERSION     DATGEM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361780"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VKQ27_00490"
     CDS             complement(<1..909)
                     /locus_tag="VKQ27_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY87436.1"
                     /translation="MSAERILIAMHDFAGGGTERIAVRLANEWSRRGRIVRIFCGHEH
                     GPSRDLVSAKVEVGEADPVIPRSLLSRGTLGVSLRQEALAWQPDVVFGTGNFHLPVFA
                     AYNAAGGSGAKTVCKLSNPLGLAGFVRLLAPAHSLGLRMLTKDIDALVAMSPALRNEA
                     AHILKRPDLLMAPEPILGGTPAARLAAWQGQDPSPLIVCAGRLERQKNFSLALETFAA
                     LPPSLDARLAILGEGSQRRLLERQARRLGIADRVHMPGHVPDVATWLRRATAFLSTSH
                     YEGYPAVLVEAIAAGIPVITTDCSPAI"
     gene            1277..2287
                     /locus_tag="VKQ27_00495"
     CDS             1277..2287
                     /locus_tag="VKQ27_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87437.1"
                     /translation="MTRLAVLFAALAASSTPALAVAAPAYRVAGSIAVPDGGWDYASV
                     DPATNRLFIARSGSVTAISLGSDRKVTSFGTIARGHAVVPLPDEHLAVTSGKDDSLRL
                     FGQEGGEQTGTVTVGSGPDAAIADGMTGNVLVMNAHGGTVSVVDPRAQKVVRSIALQP
                     GLEFAALDRHDTLFVNNEDLNHIDVVTPATGAVAAPIALTGCEGPSGLAYDAQTDRLI
                     AACANGKAAVIDAARRRLVGLLDIGKGPDAVLLDTKRRLAFIPCGRDGTLVEIALDGT
                     IGPRVIATIPTEIGARTGALDPRDGAIYLPTARFGPPPAAGLRPAPIPGSAHVLVVRP
                     AR"
     gene            2307..>2611
                     /locus_tag="VKQ27_00500"
     CDS             2307..>2611
                     /locus_tag="VKQ27_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87438.1"
                     /translation="MRILVAEDDAETARFIEHGLGQIGHVVGVVGDGHDALREATTHH
                     WDVIVLDRMLPSVDGVDIVRRMRTDEVTTPVLLLTALGRIEDRVEGLEAGADDYLVKP
                     "
BASE COUNT          478 a          841 c          840 g          452 t
ORIGIN      
        1 gatcgcagga gagcaatcgg tcgtgatcac cggaatgccg gccgcgatcg cctcgaccag
       61 aaccgccgga taaccttcgt aatgcgatgt ggagaggaag gcagtcgccc gccgcaacca
      121 ggtggccaca tccggtacat gccccggcat gtgcacgcgg tctgcgatac ccagtcgccg
      181 cgcttgcctt tccagcaacc gccgttgcga tccttccccg aggatcgcga gccgggcatc
      241 aaggctcggc ggcagcgctg cgaaggtttc gagcgccagt gaaaaattct tctgccgctc
      301 caggcggccg gcacacacga tgagtgggga aggatcctgt ccttgccatg ccgccagacg
      361 tgccgctggc gtgccaccga ggataggctc gggggccatg agcagatcag ggcgcttcag
      421 gatgtgggcg gcctcgttcc gaagggctgg actcatcgcc acaagtgcat cgatgtcctt
      481 ggtcagcatg cgcaaaccga gcgaatgcgc gggggcaagc aaccgcacaa agcccgcaag
      541 cccaagcgga ttgctcagct tgcaaaccgt tttggcaccg cttccgcctg ccgcgttata
      601 ggcagcgaac acagggagat gaaaattgcc cgtgccgaat acgacatcag gctgccatgc
      661 cagtgcctcc tgccgcaaag agacccccag cgtgccgcgc gatagcaggc tgcgcgggat
      721 gacaggatca gcttcgccta cctcgacctt tgccgagacc aaatcgcggc tcggcccatg
      781 ttcatggccg cagaaaattc gaacaatcct tccgcgtcgt gaccactcat tggccagccg
      841 aacagcaatc cgctcggtac cgccgcccgc gaaatcgtgc atggcgatga ggatacgctc
      901 cgccgacatc cgatgcatgc tcctgaaatt attactgaag tgacgggatc aaacgggacg
      961 acgtcccgtt agctgggctt ggtttcaatt ttgacgtggt ggtcgcgggc ggttctggct
     1021 gatcgaaaag gacgcattcg taacgagacg tcaccaaaac ctcgtcgccg cagtcctcgg
     1081 attatttgag ctcatcagcc tgtcgtgcca gccgtccaga cgagcgcgta acggccccgg
     1141 cgcgggaggt gataaggaag ggtcggctcc aaccggcgga atcatacaat ttcccaatcc
     1201 gccattcccc gctggcgcct aggcgccagc atggccatgc ttccattcca ttccattctg
     1261 atcggagcgt gtgcctgtga cccgtctcgc cgtcctgttc gcagcgctag ccgcttcctc
     1321 cactccggct ctggccgtcg ccgcaccggc ctatcgcgtt gcagggagca ttgcggtgcc
     1381 ggacggcggc tgggactatg cgagcgtgga tccggcgacg aaccgcttgt tcatagcgcg
     1441 cagcggcagc gtgacggcga tctctctcgg cagcgaccgg aaggtgacga gcttcggcac
     1501 gattgcgcgt gggcatgcag tcgtgccgct gccggacgag cacctcgcgg ttaccagcgg
     1561 caaggacgac agcctgcgcc ttttcggaca ggagggaggg gagcagaccg gtacggtgac
     1621 ggttgggagc ggccccgacg cggcgattgc cgacggtatg acgggcaacg tgctggtgat
     1681 gaatgcccac ggcggcaccg tttctgtcgt cgacccgcgc gcgcagaaag tggttcgctc
     1741 gatcgcgctc cagcctgggc tcgaattcgc ggcgctcgat cggcacgaca cattgttcgt
     1801 caacaatgaa gatctgaacc atatcgacgt ggtgaccccc gctaccggcg cggtcgcagc
     1861 gccgattgcg ctcaccggtt gcgaagggcc cagcggtctc gcttatgatg ctcagacgga
     1921 tcggctgatc gccgcctgcg ccaacgggaa ggccgcagtg atcgatgccg caagacggcg
     1981 cctcgtgggc ctgctcgata tcggcaaagg accggatgcc gtgctcctcg ataccaagcg
     2041 ccgactggcc ttcatacctt gtggccgcga cggcacgctg gtcgagattg cactggacgg
     2101 caccatcggc cccagggtca tcgcgacaat cccgaccgaa atcggcgcgc gcaccggggc
     2161 gctcgatcct cgcgatggtg cgatctacct gcccaccgcg cgctttggcc cgccgcccgc
     2221 cgctggcctg cggcctgcac cgatcccggg tagcgcgcat gtgctggtcg tgcgcccggc
     2281 gcggtgagat tggcgtggcg aactccatga ggatcctggt cgcggaagac gacgccgaga
     2341 cagcccgctt catcgaacac gggctgggcc agatcggcca tgtcgtgggt gtcgtcggcg
     2401 acggccatga cgccctccgc gaggccacca cgcaccactg ggatgtgatc gtactggatc
     2461 ggatgctgcc cagtgttgat ggcgtcgaca tcgtccgtcg gatgcgcacg gacgaggtga
     2521 cgacgccggt gctgctactg accgcgctcg gccgaatcga ggatcgcgtg gaagggctgg
     2581 aggcaggtgc cgacgactat ctcgtcaagc c
//
LOCUS       DATGEM010000022         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1246269, whole genome shotgun sequence.
ACCESSION   DATGEM010000022 DATGEM010000000
VERSION     DATGEM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246269"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VKQ27_00505"
     CDS             complement(<1..1152)
                     /locus_tag="VKQ27_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLY87439.1"
                     /translation="MMQQYLRIKAQHPDVLLFYRMGDFYEMFYDDARRAAQLLDIALT
                     QRGASAGAPIPMAGVPAVTLDSYLAKLVRKGQSVAICEQRGEPGKTKGPMEREVVRIV
                     TPGTVTDEALLDERRDNLLASVCGAGGRYGLAWLDLSAGRFSVMELGGLAALEAEIER
                     LRPAEILAPDGAQPALAGSSGSGSGSGSGLQDRPWRLRAPWHFDTESSSRALAEQFHT
                     RDLAGFGCADKPLAIAAAGALLAYVRETQKASLPHLLSITTEERDAALIMDPATRRNL
                     ELDESLAGRPELTLAGVFDHTATAMGGRLLRRWLHRPLRDHAVLKLRYHAVATLMDGA
                     RHVALAALLRSIGDLERILARVALRSARPRDLTQLRTALGALPNVHECLR"
     gene            complement(1369..1692)
                     /locus_tag="VKQ27_00510"
     CDS             complement(1369..1692)
                     /locus_tag="VKQ27_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87440.1"
                     /translation="MRLLLDESVPSRLRRSLTDHSDRTVVEAGWSGVKNGELLAFAAA
                     DFDAFVTVDKNLPFQQNLATLPIALVVLDSVSNELPALLPLVPALLRELASLKPRSYT
                     LVKAA"
     gene            2003..>2338
                     /locus_tag="VKQ27_00515"
     CDS             2003..>2338
                     /locus_tag="VKQ27_00515"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HLY87441.1"
                     /translation="MYHSTRNPVLVRTPLTRLRPTQFTVGYAEVEIKARQWGRLGKKE
                     RKIELERHVFPAVRGLGKDYFIVDHHHLGVALLEEGMKEVFVAVLDDLSWLKPPVFWR
                     TLEYRSWTHP"
BASE COUNT          446 a          717 c          774 g          401 t
ORIGIN      
        1 gcgcagacat tcgtggacgt tcggcagcgc gcccaacgcc gtgcgcagct gcgtgaggtc
       61 ccgcggccgc gcggaacgca gcgcgacccg cgccaagatc cgctcgagat cgccgatcga
      121 tcgcagcagc gccgccagcg cgacgtggcg ggcgccgtcc atgagcgtcg cgaccgcgtg
      181 atagcgcagc ttgaggacgg cgtgatcgcg cagcggccga tgcagccagc ggcgcagcag
      241 ccgcccgccc atggcggtgg cggtgtgatc gaacacgccc gcgagcgtga gttccggcct
      301 gccggccaag ctctcgtcga gctccagatt gcgccgggtt gccgggtcca tgatgagcgc
      361 ggcatcgcgc tcttcggtgg tgatggaaag caggtgcggc agtgacgcct tttgcgtctc
      421 gcgaacgtag gccagcaacg cgccggcggc ggcaatggcg agcggcttgt cggcgcagcc
      481 aaagccggcc agatcgcggg tgtggaattg ctccgccaag gcgcgcgagg atgattcggt
      541 atcgaaatgc cagggtgcgc gtagccgcca ggggcgatcc tgaagacctg agcctgagcc
      601 tgagcctgag cctgagctgc cggccagcgc cggctgggcg ccgtcgggag cgagtatttc
      661 cgccggccgc agccgctcga tttccgcttc cagcgccgcc aatccgccga gctccatgac
      721 gctgaagcgg ccggccgaca agtcgagcca ggcgagtccg tagcggccgc cggcgccgca
      781 gacgcttgcc agcaagttat cgcgccgctc gtcgagcaac gcctcgtcgg tgacggtgcc
      841 cggtgtgacg atgcgcacca cctcgcgctc catcgggccc ttcgtcttgc cgggttcgcc
      901 gcgctgctcg caaatcgcca ccgactggcc cttgcgcacc agcttcgcca gataggagtc
      961 gagcgtgacc gcgggcacgc cggccatcgg aatcggcgca ccggccgagg cgccccgctg
     1021 cgtgagcgca atgtcgagca gctgagcggc gcggcgcgcg tcgtcgtaga acatctcgta
     1081 aaaatcgccc atgcgataga acagcagcac gtcgggatgc tgcgccttga tgcgcaggta
     1141 ctgctgcatc atgggggtgt gcggcgacgc agtgtcatcc gcgggctctg cagaggtgga
     1201 gggagagttt gtgtcttgca tgtttaaaaa aataggttgc gtctatctta ccctcggctg
     1261 gttgcgaagg atcatgcatg tgatcgaacg ggtgcccatt tgcgagacga ctcgtcggct
     1321 tctttcaata tcggaggccg ggtagaccag gttccaccgc agggactgtc aggctgcctt
     1381 gaccaaggtg taagacctgg gcttaagtga cgcgagctcg cgcaacagcg cgggaacaag
     1441 tgggagcagt gccggcaatt cgttggatac ggagtcgagc accaccaacg caatgggtaa
     1501 cgtggccagg ttctgctgaa acggcaggtt cttgtccaca gttacgaatg catcgaaatc
     1561 agcagcggca aatgcaagca actcaccgtt cttaacgccg gaccagcctg cctcaacgac
     1621 cgttcgatcg gaatgatcgg tcagcgatcg acgcagccgg cttgggaccg actcgtcaag
     1681 cagcagtcgc atccagcgcc aaccgttccc ttgctgcctc gagtacggaa accgcagtcg
     1741 ctcgggagat tgaggggaag tcctcaagaa agtcctcgag cgtagacgac ccttcgagat
     1801 aatcgaagag atttttcacg ggcactcgcg taccccggaa acaaagcgcc ccactcatga
     1861 cttcagggtc gcggctaaca agtacatcat ccattttgcg ctcctgtgcc catagtttac
     1921 acgaagcgcc aaacggctgg agcagaccga gtgttccgcg cgttaagatc gcgggagcac
     1981 agttcatcca ggaggtagga gcatgtatca ctcgacccgg aatcccgtac tggtacgcac
     2041 gccgctgacg cggttgcggc cgacgcaatt caccgtcggc tatgcggagg tggaaatcaa
     2101 agcccggcaa tgggggcggc tcggcaagaa ggagcgcaag atcgaactcg agcgccatgt
     2161 atttccggcg gtgcggggac tcgggaagga ctacttcatc gtcgatcacc atcatctcgg
     2221 ggttgccctg ctcgaggagg gcatgaaaga ggtgttcgtg gcggtgttgg acgatctgtc
     2281 ttggctcaag ccgccggtat tctggcgcac gctggagtac cggtcgtgga cacatccc
//
LOCUS       DATGEM010000023        20560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_903519, whole genome shotgun sequence.
ACCESSION   DATGEM010000023 DATGEM010000000
VERSION     DATGEM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 20560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20560
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903519"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..668)
                     /locus_tag="VKQ27_00520"
     CDS             complement(411..668)
                     /locus_tag="VKQ27_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87442.1"
                     /translation="MLRQSICLACAALVATLPVNAAPPPVIGPEAEPIIIQIVDGFAT
                     RYRTAPNGMAGYTCGRRGSDMTHGLYLYGVPQLLGASGLGA"
     gene            complement(728..907)
                     /locus_tag="VKQ27_00525"
     CDS             complement(728..907)
                     /locus_tag="VKQ27_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87443.1"
                     /translation="MSVSDGDKLAAVILAAARCAATDQHEVKDYMQQYEKFLHNFEEA
                     RKKVSKMRQVKITGF"
     gene            complement(1156..1518)
                     /locus_tag="VKQ27_00530"
     CDS             complement(1156..1518)
                     /locus_tag="VKQ27_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HLY87444.1"
                     /translation="MGFLISLIENYVVVKVILVFVTFCGVLSLVGPATAILLNDPAAK
                     NRSAEFKGGGLGNLERTIAAKEQQMAALGAAILSAEAGRNETAEAARIHHSGETGLLM
                     AGVSALQASTRAALAFAT"
     gene            complement(1536..2030)
                     /locus_tag="VKQ27_00535"
     CDS             complement(1536..2030)
                     /locus_tag="VKQ27_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLY87445.1"
                     /translation="MQRSIEAPRGPYVIYGIYEKQTNYLIYIGQTHDLDERWAQHRSR
                     TRWARNGARSHLYAMMSKHGIENYRIEPIQTVPTLAEAYALERSLIEKFGTFVNGCNM
                     TEGGETPSPEACRAYAREIWQRPEFQELRKRNARRRRRARLAAVRREAKAAAAAMTND
                     RVGT"
     gene            complement(2631..2706)
                     /locus_tag="VKQ27_00540"
     tRNA            complement(2631..2706)
                     /locus_tag="VKQ27_00540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2671..2673),aa:Lys,seq:ctt)
     gene            complement(2754..4463)
                     /locus_tag="VKQ27_00545"
     CDS             complement(2754..4463)
                     /locus_tag="VKQ27_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87446.1"
                     /translation="MDWDYIIVGGGSAGSVLANRLSARSANKVLLLEAGRDTPHGKVP
                     AAILDSYPGTAYLNPAFTWNKLKVSTEVIPHNRPNEPRPPLRTYEQARVLGGGSSING
                     QLANRGAPTDYDEWNARGAAGWNWDNVLPFFKKVERDMDFDGPWHGNEGRIPVRRIFP
                     DLWPEHAKAAGETFKAAGYKFLIDQNAQFEDGYFPITISNVYERRVSAAIGYLDPGTR
                     MRENLTISTDTQVSTLLFEGMRCIGVKAQVGGRETEFRGREVILSSGAIHSPAHLLRA
                     GIGPAAHLRDVGIEVRAAVPGVGQRLQDHPAVAVAAFIKPFARIVNDYTRRHIYLALR
                     YSSKLDGIPPGDMFTVVTNKTSWHAVGEQIGSFIITVYKTYSETGEVKLASRNWSDEP
                     IVDFNLLSDQRDLERMMDGVRRFGAMHLTPTMQSVTADPFPASYSDKVRQVGLVSKKN
                     KWITDALAKALDGPAALRSYLIRTLVMEGVTLEDLMRDDEALETFVRKAAVGVWHASC
                     TCRMGSEDDPMAVTDPAGRVRAVEGLRVVDASIFPVVPCANTNFPVLMTAEKCADAIL
                     AGA"
     gene            complement(4468..4839)
                     /locus_tag="VKQ27_00550"
     CDS             complement(4468..4839)
                     /locus_tag="VKQ27_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY87447.1"
                     /translation="MPTDLRDIEAVLQTYFDGMYEGDADKLASVFHEVAHLFGVTDGK
                     LTDWPRQYWLDLVRSRKSPEQQDLKRSDWVMQIDRSSPTTAFAKVLCQNPPRYFTDYL
                     TFVKLAEGWRIVSKTYHTETR"
     gene            5041..5745
                     /locus_tag="VKQ27_00555"
     CDS             5041..5745
                     /locus_tag="VKQ27_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87448.1"
                     /translation="MNPPSNSFCFAPMFNHPPHKDEAGVVHASYDATGAFHPYMSAYE
                     KLYSKTGSVTTLKFNNHAHASDEFDAIMGQINHAVSVADAQLDAIVYFGHGWPTGLVS
                     ADIYTGKLVAFAKLIRANCVPGVTIVLYACLCGQRNTQGGCFAARLANELTDMQATVY
                     AHETAGHTTTNPYVYRFTGNQPAVAVAPRGKLRAFTKLLKSESIDAKPRSNTAFWARM
                     PFMTDEEISSEVSGYS"
     gene            complement(5763..6476)
                     /locus_tag="VKQ27_00560"
     CDS             complement(5763..6476)
                     /locus_tag="VKQ27_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HLY87449.1"
                     /translation="MANKLPVAIVFDTFGTIVDWRGSLIAELTAFGRERGVDADWVAL
                     VDAWRAAYHPSMDRVRKGQQPWTTLDNLHRSSLDKLVADFGIKGLTEEDLSHINLGWH
                     RLKPWPDAIPGLTRLKRKFIIGPLSNGNVALLTKMAKNGGIPWDVVFGSDLFGHFKPD
                     PETYLGVAKLLDLAPGEVMLGAAHNNDLANARKCGLMTGFFPRPTEYGPHQKRDFAAD
                     QEWDVVATDVEDMAAKLGC"
     gene            6574..7590
                     /locus_tag="VKQ27_00565"
     CDS             6574..7590
                     /locus_tag="VKQ27_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HLY87450.1"
                     /translation="MANRLRLAFLAAMLAGALLVPRVSHAAASREALVIGNGTYQSLA
                     PLPACLLSAHAVAAALHGAGFDVVEREDASSGATDAAIGEFAKHVADAPGTAAVIYIC
                     GYATSFNNRPFLLPVSAKIARPADVLTQGVLAKSVISALARGVIGASLAAIDVVPTPD
                     APASLGTDALTQGALPDGLGLIVASQAKLPEAPSQLATALAAALKAPQVQVGPVVTTL
                     QQQAGAAAVTVRQPVTPGYLVGSPPPPPSSPPPAPVAAAPAAPQATPAPVVTLPADDE
                     MTDADRRTVQTALTKLGYYDGKVDGIFGPDTRAAIRRYQHELGADMTGKLSAAQASKL
                     VGGK"
     gene            7610..8482
                     /locus_tag="VKQ27_00570"
     CDS             7610..8482
                     /locus_tag="VKQ27_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HLY87451.1"
                     /translation="MGLKGKFNVVMLVAFVVGLALAAGLSWHITHDNARREVLQEANI
                     MMAMASAIRDYTNNEIEPLLADQIKTRFLPHTVPSWAAQTNLRALAKQFPDYSYKEPA
                     LNPTNPADKATDWESDIIGEFNHNPSLAEFTTTRDTASGPILSFSRPIRITDKACLTC
                     HSTPSAAPASMIDLYGSSNGFGWKLGDVIGAQVVSVPMSVALDRANGAFKVNLFVLIA
                     VFAVTILLMNLLLHYVIVKPIRRMSAIASEVSLGKMDAPEFEERGKDEIASLAGSFNR
                     MRRSLANAMKLLES"
     gene            8523..9722
                     /locus_tag="VKQ27_00575"
     CDS             8523..9722
                     /locus_tag="VKQ27_00575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLY87452.1"
                     /translation="MERMLGRGAMGVIYQAHDPVIDREVAIKLIRTDLLSSDDQEDYL
                     TRFQHEARAAGRCAHPNIVAIYDFAVYEGNPFLAMEYVDGRNLTQVMAQSGRFTPAAA
                     IAVIGQVLDALACAHGFGVVHRDVKPANILLLADGRVKVTDFGISRLDNSVLTQSGSV
                     IGTPSYMSPEQCRGDVVDARSDLFSTGVVLYELLSGERPFAGRSLTEVAYRLTNEAPP
                     DLGAKYPELPPPLVAAVHRSLAKDPRARFASAAEMAATLRQLPLTDVDRTVVAPRAPA
                     EFSADTLSTIERKLAERIGPLARHLVQSAARQASSLEELHTIIARRMESSAVATGAGS
                     ARTGVNPDLLQRAERELTRYVGPIARILVKRAAGTAGSAEELWRALAAHIERDADRQG
                     FMKSLNG"
     gene            complement(9810..10574)
                     /locus_tag="VKQ27_00580"
     CDS             complement(9810..10574)
                     /locus_tag="VKQ27_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLY87453.1"
                     /translation="MRRILCLCVAALLLGSAAPSSEVVLAATPISRMQLRWWKDRYEA
                     KLDELRADHPALVFYGDSITQDYEVSGPPEWRDFAPVWQHFYGDRHAINLGFNGDATS
                     HLLWRIENGEASGISPKVAIILIGANNMGRLHWSAEDTVTGIGAIINQLHQKLPHTKL
                     LLLGVLPSDRSAWATETTIAINRMLAARYGHGSEVTYLDIGHIFMKDGRLDRSLFFDP
                     LLKSPSAPLHPTSEGQRMMAAAIEPTLASLLGDHRH"
     gene            10650..11546
                     /locus_tag="VKQ27_00585"
     CDS             10650..11546
                     /locus_tag="VKQ27_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLY87454.1"
                     /translation="MRNDLPAWRSLLFVPVTAEKFVRTGADRGADGVILDLEDAVAPA
                     EKERARTLIADAIPQVARKGGDVLVRVNRPWRLLVRDLEAAVVPGVAALMLTKVDSAE
                     HVVAVAEIVEELEKERGLPAGKLQFVVLVETAAGFFRIESIAKSHPRVVALSLGTEDF
                     TADVGMLPDPEGLLYPKQHTLFAARAAGILPMGFIGSIADFRDQEAFRAIVRRSKRFG
                     FVGASAIHPLQVPVLNEEFSPSEAEVERARRMIAAYDAAYAQGLGAVQFEGAMIDVPV
                     VQRAQNVLARAAAIAARAKVSV"
     gene            11561..12334
                     /locus_tag="VKQ27_00590"
     CDS             11561..12334
                     /locus_tag="VKQ27_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY87455.1"
                     /translation="MQANPMRAALERGELQIGTWVNMVRNPAILPLLKAAGVDFARVD
                     MEHTGADMGEIAQLALLSRALNFPIAVRPPKANREWITRLLDAGVWNLHCPQVENAAH
                     AAEIVAASRYAPRGLRGNAGHSPGTDFDMTGSAAERRAFANRQVFVTVMFETAAAFAD
                     LDAIAAMDGIDALTIGPADLAQDLGVFGTPDQARVLDEKRDLVLAAARKHGKVCAMLC
                     SSYEQAQQWKAAGALLLAYSSDSEVLHSAYAATMARIKG"
     gene            12418..14184
                     /locus_tag="VKQ27_00595"
     CDS             12418..14184
                     /locus_tag="VKQ27_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLY87456.1"
                     /translation="MHGFASLATSDRMPDGVAPSARGDIPHALQSQIDRFDVALNNLT
                     QGVCFFDGNRCLILANRRYADIYQLPHEAIRPGMTLEDIVDLRFAAGTCADMTRSEYL
                     DWRATIQVSDEPTDTIVELKSGQIISIHHRPMPDGGWVATHEDITERRLAEQQLAHMA
                     RHDPLTGLSNRAAFREAADRESARRLPGNSVAVLCLDLDHFKHVNDTFGHATGDALLC
                     AVASRLRGAIRASDMAIRLGGDEFAIVQVGVDQPSQAADLAARLIDILSHPFDLGVHQ
                     VIVGTSVGIAYSQAYGADTDALLKSADMALYRAKADGRGTYRFFEPEMNEQAQARHAL
                     ERGLRTALTNNELELFFQPILNARTQALSKFEALIRWRHPQRGLVQPAEFIPLSEDIG
                     LIVPFGQWVLQEACRVAAKWPDSISVAVNLSPVQFKEPNLVHVVGEALQASGLSPDRL
                     ELEITETVLLKNTTAVMHTLRRLRDLGVRISLDDFGTGYSSISYLRTFPFDTIKIDRS
                     FVHDIGSGTSSLAIVNAIVTLGEALGMSVTAEGVETCEQLRTLRAERCTDVQGYLFSA
                     PVPSADVPALIKRLSPADRVAA"
     gene            14301..14834
                     /locus_tag="VKQ27_00600"
     CDS             14301..14834
                     /locus_tag="VKQ27_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87457.1"
                     /translation="MAQMAYDKEGPAIGTLHEVRARFRDPDAMERAIEQLEVSGFNRA
                     DLSLPEARPPLARATPEYGAKAPSDESDARQARTLHTSGAAAAVGMAAAGVVIATGGA
                     AAPAVAAAVVGGGLAGGAAYALSSAANNGEQEDRERKAASGTLILSVHAVSEEKRGEA
                     AAILQAAGGTDVEVVDR"
     gene            14831..15055
                     /locus_tag="VKQ27_00605"
     CDS             14831..15055
                     /locus_tag="VKQ27_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87458.1"
                     /translation="MNDDRHDRTVEDSFPASDPPANTGVTGPRAAKRRPAPHKRDDDS
                     RPKGTPNHDRHATETAYQWEHEERPGKKGR"
     gene            complement(15273..16244)
                     /gene="gcvA"
                     /locus_tag="VKQ27_00610"
     CDS             complement(15273..16244)
                     /gene="gcvA"
                     /locus_tag="VKQ27_00610"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HLY87459.1"
                     /translation="MTPLPCYESSAPHRLTAARIVTIIPHIMSEFSSHRLPSLHALRS
                     FEAAARHANFSRAAAELHITHGAISHQMKALERELGVTLFHRRSRGVELTAQGRDLAA
                     TMHEALDRIARGVTALRAQPHRALTVSMLPAFATHWLIPRLADFNRKHPEIDINIRAS
                     QTLTDFATDDVDLAVRYGGGHWPHLHAEQLAQEDVFPVCSPRFNNGHPPATLADLAKA
                     RLLHTPLQPWDEWFRALGVHTNLPRRGMTFSETDILLSAAIDGLGVALSRRLLAQPAL
                     DTGRLVRPVPHSVRAERSYFIVYPQQLAPAPRLLIFRDWLLMQARKT"
     gene            16243..16776
                     /locus_tag="VKQ27_00615"
     CDS             16243..16776
                     /locus_tag="VKQ27_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLY87460.1"
                     /translation="MTRAFTALAFTPSVRAAQLRHGSRERMEKLAAREPDRATLPEEM
                     QERLRRANSIIVGTASLEGWPHIQHRGGPPGFIKVLAPDELVFGDYRGNRQYITVGNL
                     AENSRMFLLLMDYAAGTRLKIWGRGTVVEDDPALLERVRDPDYPAAVERVIRMHVEAW
                     DTNCDSHIPKLVPAEAA"
     gene            16778..17989
                     /locus_tag="VKQ27_00620"
     CDS             16778..17989
                     /locus_tag="VKQ27_00620"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HLY87461.1"
                     /translation="MADAVAGVVAVRRTTHLGVVAGLAMGPAVGLGLGRFAYALLLPA
                     MRSDLGWSYAQAGAINTANAAGYLVGALIAAPIAARIGDKRAFLVGLLLATASLLANG
                     LSADYAVLAVLRAVAGAAGALSLITGGALASAAGSGGDRGRPALALGVYFGGAGLGML
                     VSAAVLPVLVAEVGWRSGWLALGLLGVSAVVVALPALARAPEVALREVVRGPALSLSS
                     IRGLQAILVSYVLFGAGYIAYTTFIVAYLRNRLGFAAADVTLFWGCAGLAATGAGFVW
                     GPVLSRLSGGRGVALANAVVMVGAVLPVLLPDRAAVYVSAVVFGGSMLIVPTSVTAFV
                     RKAVAARMWTGAIAALTVGFAIGQCIGPWVSGVVSDASYGVAGGLLLSGGVLAGAALT
                     ALMQREPCAAS"
     gene            complement(18331..18621)
                     /locus_tag="VKQ27_00625"
     CDS             complement(18331..18621)
                     /locus_tag="VKQ27_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLY87462.1"
                     /translation="MTKTLTLREANQGFSRCIREMEAGEEFVITRNGTSVARLSPVAT
                     RRVLTPEQQAALERTRKRMEQGWDIGAGALDRDAGRTAPHNRPGHHQSGGAG"
     gene            18897..19913
                     /locus_tag="VKQ27_00630"
     CDS             18897..19913
                     /locus_tag="VKQ27_00630"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLY87463.1"
                     /translation="MSLQHPTVAIVGATGAVGAELIGCLERRNFPLERLRLFASGRSA
                     GRKLLFRGQGIVVEELNEDAFPGVDIALFSAGSGVSRRFAPMAVRQGAVVVDNSSAFR
                     MEPGVPLVVPEVNAEAIGRHMGLIANPNCVAIVAVVALWPLHRHTPIRRVIAATYQAA
                     SGAGAAAMEELRESTAAYLAGTEYERRVLAHPYAFNLFSHDTRVDPASGYNEEETKVM
                     RELRKIMSEPEMRIGITCVRVPVLRAHSMALTVEFERPVAPDAARAVLCEAQGLRIVD
                     DVAENHFPMPNEASGRDEVLVGRIRTDVSDPSGCSLALFVAGDQLLKGAALNAVQIAE
                     RLVG"
     gene            complement(19972..>20560)
                     /gene="aqpZ"
                     /locus_tag="VKQ27_00635"
     CDS             complement(19972..>20560)
                     /gene="aqpZ"
                     /locus_tag="VKQ27_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HLY87464.1"
                     /translation="MAYAVGHISGGHFNPAVTVGLWAGGRCNSADVVPYVIAQVVGAI
                     AAAAALYWIASGKPGWVPAGFAANGYGDLSPGKYSLGACLAMEVLMTFFFLLIIIGCT
                     SKGAAVGFAGIPIGLALTLIHLVSIPVTNTSVNPARSTGPALFAGGEYVAQLWLFWLA
                     PIVGALIAGFVTRWLHEPVVITETVMVIEESERTA"
BASE COUNT         3576 a         6220 c         6954 g         3810 t
ORIGIN      
        1 ccggcgtctt ggcgccgcgt ctccagggca gccaccatga cgatgggaaa caggagcgtt
       61 ccgtagaata ccacgccgag ttttgcgact atcgggaagc gattgaacac ctcggcgacc
      121 acaaatgacg gttgcgtgcc cagtgcgcca tctaatgcaa ccactctcgc gtcatacacg
      181 tgcggataca cacaaatcgc ctgctggccg ttttgccacc gaagtccgtc gctagccaaa
      241 taccactgcg caggtgctac agtgacaacg agtgtcgcgc ggtatcggcg gatccctcgg
      301 tactgtcgcc ctctcccagc gtggttgccc tggctgattg cgcttgtgct caccagttgt
      361 gccataccgc ccccgcgcaa tgacgacact ggcccctatc acatcacgaa ctacgccccg
      421 agtccgctcg cacccaacaa ttgtggcacg ccgtacaaat ataagccgtg cgtcatgtcg
      481 ctcccacgac ggccacaggt gtacccggcc atgccatttg gagcggtgcg gtagcgagtg
      541 gcgaacccgt ccacaatttg gatgatgatc ggctctgcct ccggcccgat gaccggcgga
      601 ggcgcggcgt tgacaggcaa tgttgccacc agcgccgcgc atgccagaca gattgattgc
      661 ctcagcatcc tcccttcctc cgtgaacgca tggcacaagg gcgccattcc gactaatcaa
      721 cgagaggcta gaagcccgtg atcttcactt gacgcatctt ggacaccttc ttcctcgctt
      781 cctcgaagtt gtgaagaaat ttctcgtatt gctgcatgta atccttgacc tcatgttggt
      841 cggttgcggc acatcgcgct gccgccaaga ttactgccgc caatttgtcg ccatcgctta
      901 cagacatcga cttgcctccg tttctgtcca cctacaccac cgaaacacgc tgcccttgca
      961 ggtgcgcaac ctcatcgcca acgacggatt tctgcggcag cagatcgctg ttctgcatcg
     1021 cttgcaacga gctggcgggc gccagggcgc cggacctgat aagcttgcac cagccccagc
     1081 aggcctgagg aggctagacg agcagatatg aagtcattgt taagctcgcc ttccactgcg
     1141 ccggacgtgc cttcattagg tggcgaacgc caatgcagcc cgcgtgctcg cctgcaacgc
     1201 ggagacccca gccatgagca gcccggtctc gcccgagtgg tggatgcgtg ccgcttcggc
     1261 cgtctcattc cgtccggcct ccgcactgag gattgcggcc cccaacgctg ccatctgctg
     1321 ttccttcgcc gcgatggtgc gttccaggtt cccgaggccg ccgcccttga actcggcaga
     1381 cctattcttc gcagccgggt cattgagtag gatggctgtt gcggggccaa ccagcgagag
     1441 gacaccacaa aacgtcacaa acacgagtat gactttgaca acgacatagt tttctatcag
     1501 tgaaataaga aaacccacgg aagaaggggc tcaggtcatg ttccgacacg atcattcgtc
     1561 atagcggctg cggcagcctt cgcctcgcgc ctcactgccg ccaaccgggc tcgacgccgt
     1621 cgtcgcgcgt ttcgctttcg tagttcctga aattctggcc tctgccaaat ttccctcgcg
     1681 taagctctgc acgcttccgg gctaggagtt tcgccacctt cggtcatatt gcagccgtta
     1741 acgaacgtgc cgaacttctc gattaggctg cgttccaggg cataggcctc ggcgagggtc
     1801 ggaacggttt gaatcggctc tatgcgatag ttctcgatgc cgtgcttgga catcatggcg
     1861 tagaggtgac tgcgcgcacc gtttcgagcc cagcgcgtcc tgctccggtg ttgggcccag
     1921 cgctcatcta aatcatgtgt ttggccgatg taaatcagat agttggtttg cttttcatag
     1981 atgccataaa tgacatatgg ccctctaggc gcctcaatgg atcgctgcat cacgatagac
     2041 tccgctcgcc tccgggagag cggcctccta tagaggcatc tctccacatt cccatggagt
     2101 cggcggacgc ttttgcgagg cgaccaaatt gcgccgtatg gtccgcccga tgtctggcga
     2161 tgatacagac caagctataa tcaatcttaa acgctaccga cgccccagca catcgattca
     2221 tcggcctaat gcaagcgcca tgcgcggcta cgcacagcag tatggcgtgg cctccagggc
     2281 ggcgctcaat ttttggtcga cgtcattcgt cactaggtca acttatggct gacatgcccg
     2341 gagacttgcg gttccgggcc ctacgggcca cagcaatgac cgagatgggc ggcgcggggg
     2401 ttggcgtgat cgccttcggc gcgcactctg gccatctgac gcctcagatg gtccggcggg
     2461 atcagcgccg caccacagag caatgtgtcg cggcggcgag acagcgccag gagcatcccc
     2521 aggcgcgaga cagcgctgca tcctggcgtt gagtcgaggg aacaggactg aaacgcctag
     2581 ggcagccgtc ggggaagtcg gggaagccac ctcgactaag tcattgatat tggtgggcgc
     2641 acaaggactc gaaccttggg cccgctgatt aagagtcagc tgctctacca actgagctat
     2701 gcgcccgacc aaggggcggg cctatagcaa cagccgcccc ccttggaaac cccctacgcc
     2761 cccgcgagga tcgcgtcggc gcacttctcg gcggtcatca gcaccgggaa attcgtgttc
     2821 gcgcacggca ccaccgggaa aatcgaggca tccaccacgc gcagcccctc aaccgcgcgc
     2881 acccgcccgg cgggatcggt gaccgccatc ggatcgtcct cgctgcccat cctgcatgtg
     2941 caggacgcat gccacactcc caccgccgcc ttgcgcacga acgtctcaag cgcctcgtcg
     3001 tcgcgcatca ggtcctcgag cgtgacgccc tccatcacca gggtccggat caggtagctg
     3061 cgcagtgccg ccggtccatc caatgccttg gccagcgcat cggtgatcca cttgttcttc
     3121 ttgctcacga gcccgacctg gcggaccttg tcgctgtagc tggcggggaa aggatcggcg
     3181 gtcaccgact gcatcgtcgg tgtcaggtgc attgcgccga accggcgcac gccatccatc
     3241 atccgctcca ggtcgcgctg atccgacagc aggttgaaat cgacgatcgg ctcgtccgac
     3301 cagttgcgcg aggcgagctt tacctcgcct gtctcggaat acgtcttgta gaccgtgatg
     3361 atgaatgagc cgatctgctc gcccaccgcg tgccacgacg ttttgttggt caccacggtg
     3421 aacatgtcgc ccggcgggat accgtcgagc ttcgacgaat agcgcagcgc cagatagata
     3481 tgccgccgcg tatagtcgtt gacgatgcgc gcgaacggct tgatgaaggc ggcgacggcg
     3541 accgcgggat gatcctgcaa ccgctgtcca acgcccggca ccgccgcgcg cacttcgatc
     3601 cccacgtcgc gcagatgcgc cgcgggcccg atgccggcgc gcagcagatg cgccggcgag
     3661 tggatcgcgc ccgacgacag gatgacctcg cggccgcgga attccgtctc ccgcccaccg
     3721 acctgcgcct ttacgccaat gcagcgcatc ccctcgaaca gcagcgttga gacctgggtg
     3781 tccgtcgata tcgtcaggtt ctcgcgcatc cgggtgcctg gatcgagata gccgatggcc
     3841 gccgacacgc gccgctcgta gacattggaa atggtgatcg ggaagtagcc gtcctcgaat
     3901 tgggcgttct ggtcgatcag gaacttgtag cccgcggcct tgaacgtttc ccccgcagcc
     3961 ttcgcgtgtt ccggccacag atcagggaag atgcggcgca ccgggatgcg gccttcgttg
     4021 ccgtgccatg ggccgtcgaa gtccatgtcg cgctcgacct tcttgaagaa gggcagcacg
     4081 ttgtcccagt tccaccctgc ggcgccgcgc gcgttccact catcgtagtc tgtcggcgcg
     4141 ccgcgattgg cgagttgccc gttgatcgac gaacctccgc ccagcacgcg cgcctgctca
     4201 taggtgcgca gcggtgggcg aggctcgttc gggcgattgt gcggaatgac ctcggtgctg
     4261 actttcagct tgttccaggt gaacgccgga ttcaggtagg ccgtgccggg ataggaatcg
     4321 aggatcgccg ccggtacctt gccatgcggc gtgtcccggc cggcttccag cagcagcacc
     4381 ttgttggccg accgcgcgga gaggcgattg gccagcactg atccggcgga gccgccgccg
     4441 acgatgatat aatcccagtc catcggttca gcgtgtctct gtgtgatagg tcttggagac
     4501 gatgcgccat ccttcggcca gcttcacgaa ggtcaggtaa tcggtgaagt agcggggcgg
     4561 gttctggcac agtaccttgg cgaaggccgt ggtggggctg gacctatcaa tctgcatcac
     4621 ccagtcagac cgcttgaggt cctgctgctc gggcgatttg cggctgcgca ccaggtccag
     4681 ccagtattgg cgcggccagt cggtcagctt cccatccgtc acgccgaaca gatgcgcgac
     4741 ctcatggaag acgctggcga gcttgtcggc atcgccttcg tacatcccgt cgaaatacgt
     4801 ttgcagaaca gcttcgatgt cacgcagatc ggtcggcatg gtgtttcccc tcggcggcgt
     4861 ggacccgagc atcacgcgat ccgcggcatg cggcaaggga tgctccgccg cccttcacaa
     4921 tagcaggagg agtccctggc ccgccgatcc tgtgaaccgc gtcacttcga tgcaggggtg
     4981 catcaggtag ccgtgggtcc ggggaacaaa cggcacatcg ttcggcaaga ggtcacgtag
     5041 atgaatccgc caagcaacag cttctgcttt gcacctatgt tcaatcatcc tccccataag
     5101 gatgaagcag gtgtcgtgca cgcctcatac gacgcgacgg gggcattcca cccgtatatg
     5161 tcggcatacg agaagctcta ctcgaagacg ggtagtgtca cgacgctcaa attcaacaac
     5221 catgcccatg cgtctgacga gtttgatgcg atcatggggc agatcaacca cgcggtcagc
     5281 gtcgcggatg cacagttgga cgcgatcgtc tacttcggac acggctggcc cacgggcttg
     5341 gtgtctgcag acatctatac gggcaaactt gtcgcgttcg caaagctgat cagggccaac
     5401 tgcgttccgg gtgtgacgat cgtgctctac gcgtgtctgt gcggacagag gaatactcag
     5461 ggcggatgct ttgctgcacg tttggctaat gaactgaccg atatgcaagc gacagtgtat
     5521 gcacacgaga ccgccggaca cacgacgacg aatccgtatg tctatcggtt caccggcaac
     5581 cagccggcgg tcgcagtcgc accccgtggc aagctgcgcg cgttcaccaa gctgctgaaa
     5641 tcagagagca tcgacgcgaa gccacggagc aataccgcgt tctgggcccg catgccattc
     5701 atgaccgacg aggagatcag cagcgaagtg agtgggtaca gctagttcat cgcttaccgg
     5761 tatcagcacc ccagcttcgc agccatgtcc tccacgtcgg tcgccaccac atcccattcc
     5821 tgatcggccg cgaagtcacg cttctggtgt ggcccgtact ccgtcgggcg cgggaagaag
     5881 ccggtcatca ggccgcactt ccgcgcgttc gccaggtcat tgttgtgcgc cgcccccagc
     5941 atcacttccc ccggcgccag atcgagcagc ttcgccaccc ccagatacgt ctccggatcc
     6001 ggtttgaagt gcccgaacag atcggagccg aacaccacat cccacggaat gccgccgttc
     6061 ttcgccatct tggtcagcag ggcgacgttg ccgttggaca gcgggccgat gatgaacttc
     6121 cgcttcagtc gcgtgaggcc tgggatcgca tccggccacg gcttgagccg atgccagccc
     6181 aggttgatat ggctgagatc ctcctcggtc aggcccttga tcccgaaatc ggctaccagc
     6241 ttgtcgagcg acgaccggtg cagattgtcc agcgtggtcc acggctgctg gcccttacgc
     6301 acgcggtcca tagaagggtg ataggcggcg cgccaggcat cgaccagcgc gacccagtca
     6361 gcatccacac cgcgttcccg gccgaacgcg gtcagttccg cgatcaggct gccgcgccag
     6421 tccacgatgg ttccgaacgt atcgaacacg attgcaactg gcagcttgtt ggccatggcg
     6481 tttcctccgg acggataggc ccagccaagc ggggcagggc catgtggtca agctgttggc
     6541 cctcggcgcg ccgtcctgct aactgaaggc atgatggcga acagactacg tctggcgttc
     6601 ctggccgcca tgttggcggg cgcacttctg gtgccgcgtg tgtcacatgc ggcagcttcg
     6661 cgcgaggcct tggtcatcgg caacggcact tatcagtcgc tggcgcctct gccggcctgt
     6721 ctgctgtcgg cgcatgccgt cgctgccgca ttgcacggag cgggcttcga tgtcgtggag
     6781 cgcgaggacg cttccagcgg cgcgaccgac gctgccatcg gcgagttcgc caagcatgtt
     6841 gccgacgcgc caggcacggc agctgtcatc tacatttgcg gctacgcaac cagtttcaac
     6901 aaccggccgt tcctgttgcc ggtctccgcg aaaatcgccc ggcccgccga tgttctgacc
     6961 cagggtgtgc tggcgaaatc agtgatcagt gctctggcac gcggtgtaat cggagcctcg
     7021 ctcgcggcaa tcgatgtcgt tccgacgccg gatgctcccg ccagtctcgg cacagacgcg
     7081 ctgacgcaag gcgcgctgcc ggacggtctg ggcctgatcg tcgcgagcca ggcgaagctg
     7141 ccggaagcac cctcgcagct tgcaacggct ctggcagccg ctttgaaggc gccgcaggtc
     7201 caggtaggcc cggtggtcac cacattgcag cagcaagccg gcgccgcggc ggtgactgtg
     7261 cgccagcctg tcacgcccgg ctatctggtt ggttcgccac cgccgccacc gtcttcgcct
     7321 ccgccagccc ctgtggctgc tgcccccgcc gctcctcagg cgaccccggc gccggttgtg
     7381 acgctgccgg ccgacgatga gatgacggac gccgatcggc gcaccgtcca aacggcattg
     7441 accaagctcg gctattacga tggcaaagtg gatggcatat tcggtcccga cacccgtgcc
     7501 gccatccgtc gctatcaaca cgaactcggt gccgacatga caggcaagct cagtgccgcc
     7561 caggcgagca agctggtcgg cggcaagtga acttgcgcag gagatccgga tgggcctgaa
     7621 gggcaagttc aacgtcgtga tgctggttgc cttcgtcgtc gggctcgcgc tcgcggccgg
     7681 cctctcctgg cacatcacgc acgacaatgc gcggcgcgag gtgttgcagg aggccaacat
     7741 catgatggcg atggcctccg ccatccgcga ttacaccaac aacgagatcg aaccgctgct
     7801 ggccgaccag atcaagacac ggttcctgcc gcataccgtg ccgtcgtggg cagcgcagac
     7861 caatctgcgt gcattggcca agcagttccc cgactacagc tacaaggaac ccgcgctgaa
     7921 cccgacaaat ccggccgaca aggcgacgga ctgggaaagc gacatcatcg gcgaattcaa
     7981 ccacaatccc tcgctcgccg aattcaccac cacgcgcgac acggcgagcg gcccgatcct
     8041 gtccttctcg cgaccgatcc gcatcaccga caaggcctgt ctgacctgcc actctacgcc
     8101 atcagctgcg cctgcctcga tgatcgatct ttatggcagc tcgaacgggt tcggctggaa
     8161 gttgggcgac gtgatcggcg cgcaggttgt ctcagtgccg atgagtgtcg cgctcgatcg
     8221 cgccaacggg gcgttcaagg tcaatctgtt cgtgttgatc gcggtgtttg ccgtcaccat
     8281 cctgctgatg aacctgctgc tgcactacgt aatcgtgaag ccgatccgcc gcatgtcggc
     8341 gattgccagc gaagtaagcc tgggcaagat ggatgcaccg gagttcgagg agcgcggaaa
     8401 ggacgagatc gcctcgctgg caggatcgtt caaccgcatg cgccgtagcc tggcgaacgc
     8461 gatgaagctg ctggagagct gacatttccg aggcgctgcc tgccgagatt ggccgttatc
     8521 gcgtggagcg catgctcggc cgcggcgcca tgggcgtgat ctaccaggcg cacgatccgg
     8581 tcatcgaccg cgaagtggcg atcaagctga tccgcaccga tctgctcagc agcgacgatc
     8641 aggaggacta tctaacacgg ttccagcatg aggcccgggc cgcgggtcgc tgtgcgcatc
     8701 cgaacatcgt ggcgatctac gacttcgctg tgtacgaggg taatccgttc ctcgcgatgg
     8761 aatatgtcga tggccgcaat ctgactcagg tgatggcgca gtccggccgt ttcacgcccg
     8821 cggccgcaat cgcggtgatc ggccaggtgc tggacgcgct ggcctgcgcg cacggctttg
     8881 gtgtcgtgca tcgcgatgtg aagcccgcca acatcctgct gctggcggat gggcgggtga
     8941 aggtgacgga tttcggcatt tcccgcctcg acaattccgt gctgacgcaa agcggctcgg
     9001 tcatcgggac gcccagttat atgtcgcccg agcaatgccg tggcgacgtg gtggatgccc
     9061 gcagcgacct gttttccacc ggtgtcgtgc tgtacgagtt gctgagcggc gagcggccgt
     9121 tcgcggggcg cagtctgacc gaggtcgctt accggctcac caacgaagcg cctccggatc
     9181 ttggcgcgaa gtatcccgag ttgccgccgc cgctggtcgc ggccgtgcac cgctcactgg
     9241 cgaaggatcc gcgcgcccgg ttcgcatcgg ccgcggagat ggcagccacg ctgcggcagc
     9301 tgcccttgac cgacgtggac cggaccgtcg tggcaccacg cgcgcccgct gaattcagtg
     9361 ccgacacgct cagcacgatc gaacgcaagc tggctgagcg cattgggccc ctcgcgcggc
     9421 atctggtgca gagcgccgcg cgccaggcga gctcgctgga ggagctgcac acgatcatcg
     9481 cccggcgcat ggagagttcc gctgtggcga cgggggccgg gtcagcgcgt accggtgtca
     9541 atccggatct tttgcagaga gccgagcgcg aactcacgcg ctatgtcggg ccgattgcgc
     9601 ggatcctggt gaagcgggcg gcaggcaccg ccggttcagc cgaggaactc tggcgcgccc
     9661 tggcagcgca tatcgaacgc gatgccgatc gtcaggggtt catgaagtcg ttgaacggct
     9721 gacatccggc cttgcggatc gcgaagcaat cccgtccgag attgcttccc cccgccttcg
     9781 cgggggccgc gatgacactg ggaggcgggt cagtggcggt ggtcgccgag cagtgaggcg
     9841 agcgtgggtt cgatcgctgc ggccatcatg cgctggcctt cggaggtggg atgcagcggg
     9901 gcgcttggtg acttcagcag cgggtcgaag aagaggctgc gatccagccg gccgtccttc
     9961 atgaagatgt ggccgatgtc gaggtaggtg acctcgctgc cgtgtccgta acgggcggcg
    10021 agcatgcggt tgatcgcaat cgtcgtttca gttgcccagg cggagcgatc ggatggcagc
    10081 acgccgagca gcagcagctt ggtgtgcggc agcttctggt gcagctggtt gatgatggcg
    10141 ccgatgccgg tgacggtgtc ctcggccgac cagtgaaggc ggcccatgtt gttggcgccg
    10201 atcaggatga tcgcgacctt gggggagatg ccgctcgctt cgccgttctc gatgcgccag
    10261 agcagatgcg aggtcgcatc gccgttgaag ccgagattga tcgcgtggcg gtcgccatag
    10321 aaatgttgcc ataccggtgc gaagtcgcgc cattcgggcg ggccgctgac ctcgtagtcc
    10381 tgggtgatgg agtcgccata gaacaccagg gcggggtggt cggcgcgcag ttcgtcgagc
    10441 ttcgcctcgt accggtcctt ccaccagcga agttgcatgc gcgatatcgg cgtcgcggcc
    10501 agcacgactt cggaggacgg tgcggcgctg cccagcagca gggcggcaac gcacaggcag
    10561 aggatacggc gcatgctggc gggtccatcg cgattcgtgg gcggcgcctc tatagtcggg
    10621 cggatcaaac gtcgaaaggg ctttgctcga tgcgcaatga tctgcctgcc tggcgctccc
    10681 tgctgtttgt tccggtgacg gcggagaagt tcgtgcgcac cggcgcggat aggggcgccg
    10741 acggggtcat cctcgatctg gaggatgcgg tggcgccggc ggagaaggaa cgtgcccgca
    10801 cgctgatcgc cgatgccatc ccgcaggtgg cgcgcaaggg cggcgacgtg ctggtgcggg
    10861 tgaaccgtcc gtggcggctg ctggtgcgcg acctggaggc ggcggtggtt cccggcgtcg
    10921 ccgcgctgat gctgacgaag gtggacagcg ccgagcacgt ggtcgcggtc gcggagattg
    10981 tcgaggagct ggaaaaggag cgcggactgc cggcgggcaa gctgcagttc gtcgtgctgg
    11041 tggagacggc ggcggggttc ttccggatcg agtcgatcgc gaaatcgcat cctcgggtgg
    11101 tggcgctgag tctggggacc gaggatttca ccgctgatgt cggcatgctg cccgatccgg
    11161 aagggttgtt gtatccgaag cagcacacgc tgttcgcggc gcgggcggcg ggaatcctgc
    11221 cgatggggtt catcggctcg atcgctgact tccgcgacca ggaggcgttc cgcgcgatcg
    11281 tgcgacgttc caagcgcttc ggatttgttg gcgccagcgc gatccatccg ctgcaggtgc
    11341 cggtgctgaa cgaggagttc tcgcccagcg aagcggaggt ggagcgggcc aggcgcatga
    11401 tcgccgcgta tgacgctgcg tatgcacaag gccttggtgc ggtgcagttc gagggcgcga
    11461 tgatcgatgt gccggtcgtg cagcgcgcgc agaacgtgct ggctcgcgcg gcggccattg
    11521 cggcaagggc gaaagtttca gtgtgaaggt ggagggagcg atgcaagcca acccgatgcg
    11581 cgcggcgctg gaacgcggtg aattgcagat tggcacctgg gtcaacatgg tgcgcaatcc
    11641 ggcgatcctg ccgctgctga aggcagcggg agtggatttc gcgcgcgtcg acatggagca
    11701 cacgggcgcc gatatgggcg agatcgcgca gctggcattg ctgtcgcgcg cgctgaattt
    11761 cccgatcgcg gtgcgcccgc caaaggccaa tcgcgagtgg atcacgcggc tgctcgatgc
    11821 gggcgtgtgg aacctgcatt gcccgcaggt agagaacgct gcgcatgcgg cggagattgt
    11881 cgcggcgtcg cgttacgcgc ctcgggggct gcgcggcaat gccgggcata gtcccggcac
    11941 ggatttcgac atgacgggca gtgccgcgga acggcgcgcc tttgccaacc gccaggtgtt
    12001 cgtgactgtc atgttcgaga ccgcggcggc gtttgctgat ctggacgcga tcgcggcgat
    12061 ggatgggatc gatgcgctga cgatcgggcc ggcggatctg gcgcaggatc tgggtgtgtt
    12121 cggcacgccc gatcaggcgc gcgtgctgga cgagaagcgg gaccttgtac tggcggcggc
    12181 acgcaagcac gggaaggtgt gcgccatgct gtgctccagc tacgaacagg cgcagcagtg
    12241 gaaagcggcc ggcgcgttgc tgctggccta ttccagcgac agcgaggtgt tgcacagcgc
    12301 ctatgccgca accatggcgc gcatcaaggg atgatctgaa accgttctga tactagcggt
    12361 catgcatagc ggtcccgttg cccgcattca cgcggggggt gtccgggacg ttttctaatg
    12421 catggattcg cctcacttgc gacgtctgat cgcatgccgg acggtgtagc gcccagtgca
    12481 cgcggcgaca taccgcatgc cctgcaaagc cagatcgacc ggttcgatgt ggcgctcaac
    12541 aatctgaccc aaggtgtctg cttctttgat ggcaaccggt gcctgattct ggcgaaccgg
    12601 cgctatgcgg acatctacca gctaccccat gaggccatcc ggccgggaat gacactggag
    12661 gatattgtcg atctgcgttt cgcggcgggg acctgcgccg atatgacgcg gagcgaatat
    12721 ctggactggc gcgcgacaat acaggtctcg gatgagccta ccgacacgat tgtcgaactg
    12781 aaaagcggcc agatcatatc gatccatcat cggccgatgc cggacggcgg ctgggtcgcg
    12841 acgcacgagg acataacgga gcgccgtctc gccgagcagc aattggcaca tatggcgcgc
    12901 catgatccgc tgaccggcct gtcgaaccgg gcggcattcc gcgaagctgc ggacagggaa
    12961 tctgcacgtc gcctgcccgg caacagcgtg gccgtgcttt gcctcgatct tgaccacttc
    13021 aagcatgtca atgacacctt tgggcatgcg acaggcgatg cactgctgtg tgccgtcgcc
    13081 tcgcggcttc gaggtgcgat tcgcgcttct gacatggcta tccgtctcgg gggcgatgaa
    13141 tttgccatcg tccaggtcgg ggttgatcaa ccaagccagg cggcggacct ggcggctcgt
    13201 ctcatcgata tactgagcca tcccttcgat ctcggggttc accaggtgat cgtcgggacc
    13261 agtgtcggga tcgcctacag tcaggcctat ggtgccgaca ccgatgcatt gctgaagagc
    13321 gcggatatgg cgctctatcg cgccaaggcg gacggccgag gcacatatcg cttcttcgaa
    13381 cccgagatga acgaacaggc gcaagcccgg catgccctgg agaggggctt gcgcacggcg
    13441 ttgacgaaca acgagctgga gctgttcttc cagccgatcc tgaatgcccg cactcaggca
    13501 ctgagcaagt tcgaggccct gatccgctgg cggcatccgc agcgcggcct ggtacagccg
    13561 gctgagttca tcccgctgtc ggaagatatc gggctgatcg tgccattcgg ccaatgggtg
    13621 ctgcaggaag cctgcagagt ggcggccaag tggccggact ccatctcggt ggccgtcaat
    13681 ctttcgccgg tgcagttcaa ggagccgaac ctggtccatg ttgtgggcga ggcgctgcaa
    13741 gcgtccggct tatcgccaga ccgcctggag ctcgagatca ccgaaaccgt gctgctgaag
    13801 aacacgacag cggtcatgca caccctgcgc agactgcgtg atcttggggt tcgcatttcg
    13861 ctggacgatt ttggaaccgg ctattcgtcg atcagctacc tgcgcacttt cccattcgat
    13921 acgatcaaaa tcgaccggtc ctttgtgcat gacattgggt ccggaacgag ctcgctggcg
    13981 atcgtgaacg ccattgttac gcttggcgag gcgcttggca tgtcggtcac ggcggagggc
    14041 gtcgaaacct gcgagcagct caggacgctg cgggcggagc gatgcacgga tgtgcaaggg
    14101 tatttgttca gcgcaccggt gccctcggcg gatgtgcctg ccttgatcaa gaggctgagc
    14161 ccggcagaca gggtggccgc gtaggttcta ggcaaccagg cggatcgccc tcccctgagt
    14221 ttgcatatgc tcggccgggc aatgatggaa cggcccgcac aatagcgcgt ttcagttcgc
    14281 tgcgagtgaa ggagcggacg atggcgcaaa tggcctatga caaagagggg ccggcgatcg
    14341 gcacactgca tgaggtccgg gcgcggtttc gtgaccctga cgcgatggag cgtgcgatcg
    14401 agcaacttga ggtgtccgga ttcaatcgcg ccgatctgag cctgccggag gcgcggccgc
    14461 cgctggcgcg ggccacgcca gaatatggcg cgaaggcgcc ttcggacgag tcggatgcgc
    14521 gccaggcgcg cacgctgcac acgagcggcg cggcggcggc tgtcggtatg gcggccgctg
    14581 gcgtggttat cgcgacagga ggggctgctg cgccagccgt ggccgcagcg gttgtgggtg
    14641 gcgggctcgc cggtggagcc gcgtatgcgc tgagcagcgc cgcgaacaac ggtgagcagg
    14701 aggatcgcga gcggaaggcg gcatcaggca cgttgatcct gtccgtgcat gcggtgagcg
    14761 aggagaagcg cggcgaggca gccgcaattc ttcaggctgc cggcgggacg gacgtggagg
    14821 tcgtggaccg atgaacgacg acaggcacga ccggacggtc gaggatagct ttccagccag
    14881 tgatccgccg gcgaacaccg gcgtgacggg gcctcgcgcg gcgaagcggc ggccggcgcc
    14941 gcacaagcgg gacgatgact cgcgacccaa ggggacgccc aatcacgatc ggcatgcgac
    15001 ggagacggcg taccagtggg aacacgagga gcggccgggg aagaagggac ggtgagccat
    15061 cgggtggctg tggtgcggtg aagttgaatg gaaattataa cacggattac attgatattc
    15121 gcacggattt cgcggagtta ttattggggt taaagttgtg aggttcagtt tgaagtctgt
    15181 atattttggg attaatattt gccatcaacg acacgagatt gctccgcttc gctcgcaatg
    15241 acagggggta ggttctgctc cgctgatatc ggctaggttt tgcgcgcctg catcagcagc
    15301 caatcgcgga agatcaacag gcgcggtgcg ggggcgagct gctgcggata gacgatgaaa
    15361 tacgaccgct cggcacgcac gctgtgcggt accggacgga caaggcgccc ggtatcgagc
    15421 gcgggctggg ccagcaggcg acgggagagc gcaacgccga gcccatcgat cgcggcactg
    15481 agcaggatgt cggtctcgct gaatgtcatg ccgcggcgcg ggaggttggt gtgaacgccg
    15541 agcgcgcgga accattcgtc ccagggttgt agcggtgtgt gcagcaggcg cgctttcgcg
    15601 aggtcggcga gagtcgcggg cgggtgcccg ttgttgaaac gcgggctgca gaccgggaat
    15661 acgtcctcct gagccaattg ctcggcatgg aggtggggcc agtggccgcc accatagcgg
    15721 accgcgagat cgacatcgtc cgtggcgaaa tcggtgaggg tctgactggc gcggatgttg
    15781 atgtcgatct cagggtgctt gcggttgaag tcggcgagcc gcgggatcag ccaatgcgtg
    15841 gcgaaggcgg gcagcatgct gacggtaagg gcgcgatgcg gctgggcgcg cagggcggtg
    15901 acaccgcggg cgatgcgatc gagcgcctcg tgcattgtgg cggcgaggtc gcggccttgc
    15961 gcggtcagtt ccacgccccg gctgcggcga tggaagaggg tgacgccgag ctcgcgctcc
    16021 agcgccttca tctggtggct gatggcgccg tgggtgatgt gcagttccgc tgcggcacgg
    16081 ctgaagttgg catggcgcgc ggcggcctcg aagctgcgca gcgcgtgcag ggatggcagg
    16141 cggtgtgaac tgaattcact cattatgtga ggaatgatcg ttacaatccg ggctgcggtg
    16201 agcctatgtg gtgcgctcga ctcatagcaa gggagaggcg tcatgacccg ggctttcacc
    16261 gccttggcgt tcacgccttc ggtgcgtgct gcgcagcttc ggcatggctc gcgcgagcgg
    16321 atggaaaagc tggcggcgcg cgaaccggat cgcgccacgc tgcctgagga aatgcaggaa
    16381 aggctgcggc gcgccaacag catcatcgtt ggcaccgcga gcctggaggg ctggccgcat
    16441 atccagcatc gcggcgggcc acccgggttc atcaaggtgc tggcgccgga cgaactggtg
    16501 tttggcgatt acaggggcaa ccggcaatac atcaccgtcg gcaacctggc ggagaacagc
    16561 cggatgttcc tgctgctgat ggactatgcg gcgggcacgc ggctgaagat ctgggggcgc
    16621 gggaccgtgg tggaggatga tccggcgctg cttgagcgtg tgcgcgatcc ggattatccg
    16681 gcagcggtgg aacgggtgat ccgcatgcat gtcgaggcgt gggacaccaa ctgcgacagc
    16741 catatcccga agctggtgcc ggcggaggcc gcgtgacatg gccgatgccg tggcaggcgt
    16801 agtggcagtg cggcgtacga cgcatctcgg ggtagtcgcc gggttggcga tgggaccggc
    16861 ggtcgggctg gggctggggc ggttcgccta tgcactgctg ctgccggcga tgcgctcgga
    16921 ccttggctgg agctatgcac aggcaggggc gatcaacact gccaatgcgg cggggtatct
    16981 ggtgggcgcg ctgattgcgg caccgattgc cgcgcggatt ggcgacaagc gcgcgttcct
    17041 cgttgggctg ctgctggcga cagcgtcgct gctggcgaac gggctgagcg ctgactatgc
    17101 cgtgctggcg gtcttgcgcg ccgtcgcagg ggcggcgggg gcgctgtcgc tcatcaccgg
    17161 cggcgcgctg gcctcggcgg cgggcagtgg cggtgaccgt ggacggccgg ccctggcgct
    17221 gggcgtttat tttggcggcg ctggacttgg catgctggtg tcggcggccg tgctgccggt
    17281 gctggtggca gaagtgggct ggcgcagcgg gtggctggcg cttgggctgc ttggcgtgtc
    17341 agcggtggtc gtagcattac cggctcttgc gcgtgcgccc gaggtcgcgt tgcgcgaggt
    17401 ggttcgggga ccagccttgt cgttgtcgtc gatccgcggc ctgcaggcga tcctggtcag
    17461 ctacgtgctg ttcggtgccg gttatatcgc ctacacgacg ttcatcgtcg cgtatctgcg
    17521 caatcgattg gggttcgccg cggcggatgt gacgctgttc tggggctgcg ccggccttgc
    17581 ggcaaccggc gccgggttcg tgtggggacc tgtgctgtca cgactctccg gcggacgtgg
    17641 tgtcgcgctg gcaaacgctg ttgtcatggt gggggcggtg ctgccggtac tgttgccgga
    17701 tcgggccgcg gtctatgtct cggcggtggt gttcggtggg tcaatgctga tcgtaccgac
    17761 ttcggtgacg gcgttcgtgc ggaaggcggt ggcagcgcgg atgtggaccg gggcgatcgc
    17821 tgcactgacg gtggggttcg cgatcgggca gtgcatcgga ccgtgggtca gcggagtggt
    17881 gtctgacgcg agctatggtg tcgcgggcgg gctgctgctg tcgggcggcg tgctggcggg
    17941 tgcggcgctg acggcactga tgcagaggga gccttgtgct gccagttgac tgctgccagc
    18001 agggcggccg cttccgggca cagttctcgt tcgccgaacg ggttgacgat gcttaccccg
    18061 agaagcgttg tgttgtgatc gaggtcctcg gtgaggattg cggaacatcc tacctcggcg
    18121 gcggtggcga cgagcaaggc atcccaatag gacgtgcgcg ccgaggcggc catggtcagc
    18181 gcggtatcta ctgcattcgc cgaggctgtc gccgtctgga acatgctcag ccaatccatc
    18241 gcctgatcac gtgccttatc cggggcaacg acgcgtttgc gagtggcggc gtagaagaac
    18301 tccgagatcg attgcagtgt cagccagcaa tcaaccagca ccgcccgatt gatgatgtcc
    18361 tgggcgatta tgtggcgctg tccggcccgc gtcccggtcg agtgcaccgg cgccgatgtc
    18421 ccagccttgt tccatgcgct tccgagtgcg ctcaagcgcc gcctgctgtt ccggcgtcag
    18481 tacccggcgc gtggctacag gcgacaggcg cgccacggat gtgccgttgc gggtgatgac
    18541 aaattcctcg ccggcttcca tctcgcggat gcagcgggag aagccctgat tggcttcgcg
    18601 gagggtgagc gtcttggtca tacggcttat gcatgaacgc gaagtgctac ttctcaagcg
    18661 atccggtcgt cggagaaatg atggtttacc gcctcgctgg ccgctgctat gaaggtggca
    18721 tgttctgtcg cgtaaacaag cgtacgtgga tcatcacgaa gcccttcggt ggggcgtcgt
    18781 gggcgtacgc atgttgatgg cagcgacata gcgtcacccc aaggccccgc cggaaacgga
    18841 cggggccttt gagtttcagg cacctcgtcc tccggcctaa cgaacggagc gagtgcatga
    18901 gcctgcaaca tcccactgtc gccatcgttg gcgccaccgg cgcggtcggc gccgaactca
    18961 tcggctgcct ggaacggcgg aactttccac tggaacgatt gcggctgttc gcatcgggcc
    19021 ggtcggccgg gcggaagctg ctgttccgcg ggcagggcat cgtggtggag gaactgaacg
    19081 aggacgcatt ccctggcgtg gatatcgcgc tgttctccgc cggcagtggc gtgtcgcgtc
    19141 ggttcgcgcc gatggcggtg cggcagggag cggtggtggt ggacaattcc tcggcgttcc
    19201 ggatggagcc gggggtgcca ctggtggtgc cggaggtgaa cgccgaggca atcggcaggc
    19261 acatgggcct gatcgccaac cccaactgcg tggcgatcgt ggcggtggtg gcgctgtggc
    19321 cgctgcatcg gcacacgccg atcaggcggg taatcgcggc gacctaccag gcggcgtcgg
    19381 gtgccggggc ggcagcgatg gaggaattgc gcgagtccac ggcggcatat ctggcgggga
    19441 ccgagtacga gcgccgcgtg ctggcccatc cctatgcgtt caacctgttc agccacgaca
    19501 cgagagtgga tccggcgagc ggctacaacg aggaagaaac gaaggtcatg cgtgagctgc
    19561 gcaaaatcat gtccgagccg gagatgcgga tcgggatcac ctgcgtacgg gtgccggtgc
    19621 tgcgagcgca ctcgatggcg ctgacggtgg agttcgagcg gccggtggca cccgatgcgg
    19681 ctcgggcggt gctgtgcgag gcgcaggggc tacggatcgt ggatgacgtc gcggagaacc
    19741 actttccaat gccgaacgag gcctcggggc gtgatgaggt tctagtcgga cgcatccgta
    19801 cggatgtgag cgatccgtcg gggtgctcgc tggcactgtt cgtcgcaggt gatcagctgc
    19861 tgaagggcgc ggcgctgaac gcggtgcaga tcgccgaacg gctggtcggg tagtggcgct
    19921 tctctatgat ggtcgggtga ccgacccggc catcacgggg cttctgagtt ttcaggcggt
    19981 gcgctcggac tcctcgatca ccatgacggt ctcggtgatc acgacgggct cgtgtagcca
    20041 gcgcgtcacg aacccggcga tcagtgcgcc gacaatgggt gccagccaga acagccagag
    20101 ctgcgccacg tattcgccgc ctgcgaacag ggccgggccg gtgctgcgcg ccgggttcac
    20161 cgaggtattg gtgaccggga tcgacacgag gtggatcagc gtcagcgcca gcccgatggg
    20221 tatgcctgcg aagcccaccg cggcgccctt cgacgtgcag ccgatgatga tgagcaggaa
    20281 gaagaaggtc atcaggacct ccatggcgag acaggcacca aggctgtact tcccggggct
    20341 caggtcgcca tatccgttcg cggcaaagcc cgccgggacc cagccgggct tgccggatgc
    20401 gatccagtaa agcgcggcgg cggcggcgat ggcgcccacc acctgggcaa tcacataggg
    20461 gaccacgtca gccgagttgc agcgtccgcc ggcccatagt ccgacggtga ctgcggggtt
    20521 gaagtggccg ccggagatgt gcccgaccgc ataggccatc
//
LOCUS       DATGEM010000024         4094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1018958, whole genome shotgun sequence.
ACCESSION   DATGEM010000024 DATGEM010000000
VERSION     DATGEM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018958"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VKQ27_00640"
     CDS             complement(<1..692)
                     /locus_tag="VKQ27_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87465.1"
                     /translation="MTSMTGVSRAGWTAASLATDPDWTFSLTDPSRRDLTDAVLRAQD
                     PAKTLFDYRRADFDLGAAKETLAAAFNEAKHGRGIALVRGLPRDTLDEAQFELLTWAI
                     GLHEGVARPQGKASQYISAVRDAGTTYRSASGRGYSSNAELDFHTDSADVVALTCFNR
                     AKAGGRSMVTSSVAAYQRLEMEHPDLAEALREPFYFSRQAEQAADEAPFYPNPIFDTC
                     EGRLFSKWNRNRL"
     gene            complement(735..1172)
                     /locus_tag="VKQ27_00645"
     CDS             complement(735..1172)
                     /locus_tag="VKQ27_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY87466.1"
                     /translation="MSDSYLPDGLPLPAAGDALSAPYWEGTRQGKLRIQKCRGCGAWQ
                     WGPEWICHRCHSFDLAWQDVAGHGRIYSYQRPHHPVHAALSGHGPYIVVVVELPDAGN
                     VRMVGNLLGDPRQVVQFGAAVQAVFEPHDGADPPFTLVQWRTT"
     gene            complement(1169..2353)
                     /locus_tag="VKQ27_00650"
     CDS             complement(1169..2353)
                     /locus_tag="VKQ27_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87467.1"
                     /translation="MTERTLRGRTAIVGIGETTYYRHGQSPDAEFKLALQAVVNACKD
                     AGISPHDIDGFASYGNDRSEAGRLAAALGTNELRFSNMFWGGGGGGVCGAVGNAAAAV
                     ATGMADCVVAFRSLAQGQFARYGRSGGAPIAAGDDQFLFPYGVMSPAQRFAMKVTRFM
                     HDHGIRQEALRAISLACYRHAQNNPNAVMRGRPLDATKYDDSRWIIEPFFHLYDCCQE
                     NDGAAAVIVVPAERARDFPNKPAMVLACAQGCDHRSAAPVHNMPNYPSSHFAQLAPRL
                     YDMAKLRPADMGIVQCYENFTGGVLMSLVEHGLVRAEEANEFLVPENLYAPDGGMPLN
                     TSGGNLAECYMHGLELVIEAVRQIRGTAINQVKRNDAAIIIGGPMVTPVSSLILGSEA
                     VL"
     gene            complement(2353..3585)
                     /locus_tag="VKQ27_00655"
     CDS             complement(2353..3585)
                     /locus_tag="VKQ27_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87468.1"
                     /translation="MDFDDTPEEAEFRTEARAFLSANAARKQRSSGAHRYGDTDAAEI
                     ARAKTWQAKKADAGFAGITWPTEWGGRGASPMQQIIWNQEEADYDVPHNVFLIGLGMC
                     IPTMIAYARPEQLQRYVRPALRGEEIWCQLFSEPAGGSDVAGLRTRAEKRGDEWLISG
                     QKIWTSGAHYSDYGLLLTRSDPNVPKHAGLTAFFIDMRASGVEVRPIHQMSGASNFNE
                     VFFTDLRVPDEQRLGAPGAGWKVAITTLMNERLAVGEIRRPDLTDLIELARAVEIDGR
                     TAISDSSVRERIAEWHARSQGLTYTRFRILTALSRGETPGPENSIAKVVNAAKLQEIA
                     SFGVDLLGMAGGVMDPTLAPMHAMFQDALLSSPSHRIAGGTDEILRNIIAERVLGLPG
                     DVRVDKDVAFNQLPSGSR"
     gene            complement(3599..>4094)
                     /locus_tag="VKQ27_00660"
     CDS             complement(3599..>4094)
                     /locus_tag="VKQ27_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87469.1"
                     /translation="AAEPLNTAGTGWALVERLLDRAAILIAFEQIGGAQACLDMARLY
                     AQERYAFGRSIASFQAIKHKLADVYVATELARSNAYYGAWALSVAAADLPLAAATARV
                     SASDAFFLAAKENIQVHGGVGFTWDYDCHLYYRRAKLLALSLGSPRYWKDRVVSELET
                     RNAA"
BASE COUNT          740 a         1366 c         1256 g          732 t
ORIGIN      
        1 agccggttgc ggttccattt gctgaagagg cgtccctcgc acgtatcaaa gatcggattg
       61 ggatagaatg gggcctcatc agccgcctgc tcggcctgac gactgaaata gaaaggttcg
      121 cgcaacgcct cggcgaggtc agggtgctcc atttcgagcc gctggtaggc agcgacagag
      181 ctggtgacca tgctgcgccc tcccgccttt gcacggttga agcaggtgag cgccacgaca
      241 tcggcactgt cggtatggaa atcgagttcg gcattggacg aatatccgcg cccactggca
      301 ctgcgatacg tggtaccggc gtcacgtacc gccgagatgt actggctggc cttgccctgc
      361 ggccgcgcta cgccctcatg cagcccgatc gcccaggtga gcaactcaaa ctgcgcctca
      421 tccaacgtgt cacgtggcag ccctcggacg agagcgatcc cccgaccgtg cttcgcctcg
      481 ttgaatgccg cggcaagtgt ttcctttgcc gcgccgagat cgaagtcggc acgccgatag
      541 tcaaacaagg tctttgcggg gtcctgagcg cgcagcacgg catcggtcag gtcacggcgc
      601 gacggatcgg tcaggctgaa ggtccagtca ggatccgtcg cgagcgaggc cgccgtccaa
      661 ccggcgcgcg acaccccagt catcgacgtc atgtcttatc tcccggatgg atggcgcatt
      721 cttcactgat ggcgctacgt cgtccgccat tgaaccagcg tgaacggtgg gtcagcgcca
      781 tcatgcggct cgaacactgc ctggaccgcc gcgccgaact gcaccacctg ccgcggatcg
      841 ccgagcaggt tgccgaccat gcgcacgttc cccgcatcgg gcagttcgac caccacgacg
      901 atatagggtc cgtggccgct cagcgcggca tgcaccgggt gatgcgggcg ctggtagctg
      961 tagatacgcc cgtgccctgc cacgtcctgc catgccagat cgaaggaatg gcagcggtgg
     1021 cagatccact caggtcccca ctgccaggcg ccgcaaccgc ggcacttctg aatccgcagc
     1081 ttgccttggc gcgtgccttc ccagtaaggc gcgctcaacg catcgccggc agccggtagt
     1141 ggcaggccat cgggcagata agagtcgctc atagcactgc ctccgagccg agaataagtg
     1201 aactcaccgg cgtcaccatc gggccgccga tgatgatcgc cgcgtcattg cgcttcacct
     1261 ggttgatcgc ggtaccgcgg atctggcgca ccgcctcgat gaccagttcc agtccgtgca
     1321 tgtagcattc cgccaggttg ccgccgctgg tattcagcgg cattccgccg tcgggcgcat
     1381 acaggttctc aggcaccagg aattcgttgg cctcctcggc gcgcaccagc ccgtgctcca
     1441 ccaggctcat cagcacgccg cccgtgaagt tctcgtagca ctgcacgata cccatgtctg
     1501 ccggacgcaa tttagccata tcgtacaaac gcggcgccag ctgggcgaaa tgcgagctgg
     1561 gatagttcgg catgttgtgc acgggtgcgg cactgcgatg gtcgcagccc tgcgcgcacg
     1621 ccagcaccat cgcgggcttg ttgggaaagt cgcgagcacg ctccgccggc actacgataa
     1681 ccgcggcagc gccatcgttc tcttggcagc agtcatagag gtggaagaac ggctcgatga
     1741 tccagcgcga atcatcgtat ttcgttgcat ccagcggccg gccgcgcatc accgcgttgg
     1801 gattgttctg cgcatgccga tagcaggcga gcgaaatagc ccgcaatgcc tcctgcctga
     1861 tcccgtggtc gtgcatgaag cgcgtcacct tcatcgcgaa gcgctgcgcc ggagacatca
     1921 ccccgtaagg aaacaggaac tggtcatcgc cggcggcgat cggcgcgcca ccgctgcggc
     1981 cgtagcgcgc gaactggccc tgcgccaggg agcgaaacgc cacgacacag tctgccatgc
     2041 cagtggccac tgccgcagcc gcattgccga ccgcgccaca cacgccaccg ccgccaccgc
     2101 cccagaacat gttggagaag cgcagctcgt tggtgccgag cgcggcggcg aggcggccgg
     2161 cctcactgcg gtcgttgccg taggaggcga agccgtcgat gtcatgcggg ctgatgccgg
     2221 cgtccttgca ggcattcacc acggcttgca acgcgagctt gaattcggca tctggcgact
     2281 ggccgtgacg ataataggtg gtctcgccga tgccgacgat cgctgtgcgg ccgcgcagag
     2341 tgcgctcggt catcatcgac tcccgctcgg cagttgattg aacgcgacat ccttgtcgac
     2401 gcgcacgtcg ccgggcagac ccagcacacg ttcggcaatg atgttgcgca ggatttcgtc
     2461 ggtgccgccg gcgatacggt ggcttggcga ggacagcagc gcgtcctgga acatcgcatg
     2521 catcggcgcc agcgtcgggt ccattacgcc acccgccatg ccgagcaagt caacgccgaa
     2581 cgacgcgatc tcctgcagct tcgccgcgtt caccaccttg gcgatggagt tctcggggcc
     2641 gggcgtttca ccacgtgaca gcgctgtcag gatacggaag cgtgtatacg tcagaccctg
     2701 gctgcgcgca tgccattcgg caatgcgctc acgcaccgaa gagtcgctga tcgcagtccg
     2761 tccatcgatc tccaccgcgc gtgcaagttc tatcagatcc gtcaagtcgg gacggcggat
     2821 ttcccccacc gccaggcgtt cattcatcag cgtggttatc gccactttcc atccggcgcc
     2881 aggcgcgccg agcctttgct catccggcac ccgcaggtct gtaaagaaca cctcgttgaa
     2941 atttgaggcc ccggacatct ggtggatcgg ccgcacctcc acgccagacg cacgcatatc
     3001 gatgaagaac gcagtcagcc cggcatgctt cggcacattc ggatcgctgc gcgtcaacag
     3061 cagcccgtaa tcggaatagt gcgcacccga cgtccagatc ttctgcccgc tgatcaacca
     3121 ctcgtcacca cgcttctcgg cgcgcgtacg caggcctgcg acatccgatc cgcccgcggg
     3181 ttctgaaaac agctggcacc aaatttcctc accgcgcagc gcgggccgca catagcgctg
     3241 caattgctcc ggcctggcgt aggcaatcat ggtgggaatg cacatgccga gaccaatgag
     3301 gaagacgttg tgcggcacgt cgtaatccgc ttcttcctgg ttccagatga tctgctgcat
     3361 ggggcttgcg ccacggccgc cccattccgt cggccacgtg atgccggcga aaccagcatc
     3421 cgccttcttc gcctgccacg tcttggcgcg cgcgatctcg gcggcgtcgg tgtcgccata
     3481 gcggtgcgcg ccgctgctgc gctgcttccg cgcggcgtta gcgctcagga acgcgcgagc
     3541 ctcagttcgg aactcggcct cttctggtgt atcgtcgaaa tccatcggag tgctccaact
     3601 aagcggcgtt gcgggtttct agttcggata ccacccgatc tttccagtaa cgcgggctgc
     3661 cgagcgacag cgccagcagc tttgcacggc gatagtacaa gtggcagtcg tagtcccagg
     3721 tgaaaccgac accgccatgc acctggatat tctccttcgc cgccagaaag aacgcatcgg
     3781 aagcggagac gcgcgcggtg gccgcggcta gcggcaggtc ggctgcagcc accgacagtg
     3841 cccaggcacc gtaatacgcg ttggaccgcg ccagttcggt cgcgacatag acgtcggcca
     3901 gcttgtgttt gatcgcctgg aacgaggcga tcgaccggcc gaaagcgtag cgctcctggg
     3961 cgtagaggcg agccatgtcg aggcaggcct gcgccccgcc aatctgttcg aacgcgatca
     4021 ggatcgcggc gcggtccagc aggcgttcga cgagcgccca ccccgtccct gccgtattca
     4081 gcggttcggc cgcg
//
LOCUS       DATGEM010000025         5914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_447750, whole genome shotgun sequence.
ACCESSION   DATGEM010000025 DATGEM010000000
VERSION     DATGEM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447750"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VKQ27_00665"
     CDS             <1..419
                     /locus_tag="VKQ27_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HLY87470.1"
                     /translation="VGTLHIAFAERLQGPWRPHPGNPVRRDRASTRPGGTPQVIDGRV
                     MLPVQDCSHTYGGAIRPLFFDRLDPTRVETEAGSLIAIPADFAPYVEGMHTLAAAGDV
                     TLIDVKRTELSAHGLSIELMREVRKRLLSGAGSRIR"
     gene            complement(435..656)
                     /locus_tag="VKQ27_00670"
     CDS             complement(435..656)
                     /locus_tag="VKQ27_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87471.1"
                     /translation="MGRKAGTTIWGTSVLLAGLGFIAAGASVPNEQTVALGFFLLLPA
                     AAVLLGTYWPKPQPSNLISMAAYRSRHHL"
     gene            1144..2064
                     /gene="cyoA"
                     /locus_tag="VKQ27_00675"
     CDS             1144..2064
                     /gene="cyoA"
                     /locus_tag="VKQ27_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HLY87472.1"
                     /translation="MNERTLRRLGSRLAWLAPMITLGGCSWDLLDPQGNIGVQNLSTL
                     TWATLLMLIVVIPVIILTIAFAWRYRSSNLAADYAPDWSHSTKIEIVVWLVPALIVAS
                     LAVIAWKTTHDLDPYRPIASKEKPIEVEVVSLDWKWLFIYPEYGVASVNEMALPVGRP
                     VHFKITSDTVMNSFFIPQLGSQIYSMAGMQTQLNLVADKAGSYKGISANFSGNGFADM
                     RFEAKAMDQKGFDAWIAAAKASPASLDMAGYAALEKASEHDPVRFYGAVTPKLFDAVL
                     MKDMHDNAPAEGKAHSQITSMQMPAPAQQD"
     gene            2070..4052
                     /gene="cyoB"
                     /locus_tag="VKQ27_00680"
     CDS             2070..4052
                     /gene="cyoB"
                     /locus_tag="VKQ27_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HLY87473.1"
                     /translation="MDMSILFGRLTLDAIPLHEPIIMGATAFMGLVGLGVVYAIHSNR
                     FWVPLWKDWLCSVDHKKIGIMYIILALVMLLRGFSDAIMMRSQQAMAAGGTQGYLPPE
                     HFDQVFTAHGVIMIFFMAMPFVIGLMNVVVPLQIGARDVAFPFLNSLSFWLTVSGALL
                     INASLAIGSFARTGWLAYPPLSELAYSPDVGVDYYIWALQISGVGTTLAGVNFIATIL
                     KMRAPGMDLMKMPIFTWTTLCTNMLIVAAFPVLTGTLALLSLDRYLGMHFFTNDHGGN
                     PMNYVNLIWMWGHPEVYILMIPLFGAFSEIIATFSGKPLFGYKSMVYATMGIAGLSFL
                     VWLHHFFTMGSGANVNTFFGITTMIISVPTGVKIFNWLFTMYKGRVRMTVPVMWTIGF
                     MLTFTIGGMTGVMLALPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAAMNYWFPKAFG
                     FTLNETLGKAAFWCWFIGFYLAFMPLYALGFMGATRRLQHYADLAWQPYFIVAACGAA
                     VILLGIVFQVAQLVVSIRDREQNRDLTGDPWNGRTLEWATSSPPPFYNFAKTPKVEAL
                     DAWHDMKKKGISPLPDTFAPIHMPRNTWAGFVIGIAGVPLGFALIWHIWWLAGVSFIG
                     MVGAAIVHSFNEDRDYYVPADEVAEIEGRIALAKVA"
     gene            4054..4674
                     /gene="cyoC"
                     /locus_tag="VKQ27_00685"
     CDS             4054..4674
                     /gene="cyoC"
                     /locus_tag="VKQ27_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HLY87474.1"
                     /translation="MNSITMGVPHHGHHDDHEHHDQFGTVQLGFWIYLMTDCVLFCTL
                     FATYAVMSTAFDGGPNPHTIFELPYVAVETALLLFSSLTYGIAMIGAHQGKRGMTMGW
                     LLVTAMFGLGFLGMEIHEFSKLIAEGAGPDRSGWLSSFFTLVGTHGLHVTSGLVWMTV
                     MLVQLAKSGLNETTIRRLTCLSLFWHFLDIVWIGVFSFVYLMGVSA"
     gene            4671..5015
                     /gene="cyoD"
                     /locus_tag="VKQ27_00690"
     CDS             4671..5015
                     /gene="cyoD"
                     /locus_tag="VKQ27_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HLY87475.1"
                     /translation="MSAHSHSDAHASHGGLKPYAIGFVLAVLLTALPFGAVMKGMVSG
                     VQAVELIMGTAVVQILVHLHFFLHIDRKTDRWTLQALFFTIFVLGVIVSGSLWVMFHL
                     NHNMMPMMPIDG"
     gene            complement(5085..5870)
                     /locus_tag="VKQ27_00695"
     CDS             complement(5085..5870)
                     /locus_tag="VKQ27_00695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87476.1"
                     /translation="MDLQLKGKTALVTGSTAGIGLAIAERLAAEGVEVTIAGRNQAKL
                     DEAAARIAKLGKVNAVLADPATAEGAATLIAATPEVDILVNNLGIYEAKAFTDISDAD
                     WHHFFEVNVVSGARLARHYFPKMLEKNWGRVIFISSESSLMIPGEMIHYGMTKTAQLS
                     ISRGLAAQTTGTGVTVNSVLPGPTRSEGIVDFMRSTVSRGDAPQDEVERLFFEELRPQ
                     SLIRRLIEAEEIGNMVAFLSSPLAAATNGAAIRAEGGILPTIA"
BASE COUNT          994 a         1887 c         1871 g         1162 t
ORIGIN      
        1 aggtgggcac gctccacatc gcctttgccg agcggttgca ggggccgtgg aggccccatc
       61 ctggcaaccc ggttcgtcgt gatcgggcga gcacgcgccc gggcggaacg ccgcaggtga
      121 tcgacggccg cgtgatgctg cccgtacagg attgctcgca cacctatggc ggcgcgatcc
      181 gtccgctgtt cttcgatcgc ctcgatccca cgcgggtcga gacggaggcg gggtcgctga
      241 tcgcgattcc cgccgacttc gcgccctatg tcgaagggat gcacacgctc gccgccgccg
      301 gcgacgtgac gttgatcgac gtcaagcgca ccgagctttc ggcgcacggg ctctcgatcg
      361 aactgatgcg cgaagtgcgc aagcgcctcc tcagcggagc cggcagccgg atccgctgag
      421 gagggcgagc gtcgctagag gtggtggcgg ctgcggtaag cggccatcga aatgaggttc
      481 gacggctgcg gcttgggcca ataggtaccc agcagcaccg ccgcggcggg gagtagcagg
      541 aagaaaccga gtgcaaccgt ctgttcgttc ggaacggacg cacctgcagc aataaagccc
      601 agtcccgcca gcaggacgga ggttccccag atggtagtgc cagccttgcg acccacagcc
      661 ttctcctctc cttatgagcg cggccgctct tcgtcggacc atctactcgg ggattagtct
      721 cggataattt gtcgtgactg tcacctgcgg caggaacggc acaaaagtgc caaatcttgt
      781 tcgaggccag aaacgaatcg tcggaccatg atcgtcccgc gcgcggcggt gtagccggag
      841 gggaaaagag gtggaaagca tccatctttt tgcaacccgc ccgcctcaaa agagcaccga
      901 acgccgcgtg aggagccggc aagtgcaaat ggcgcacgct gcagtgcaga aagttaaatt
      961 gaatttcggg ggaaatgcag ccacggcgcg cgccatggaa ccagtgcgcc agagccgcgc
     1021 cggcagtcgc atgcttgccg cgccatagcc gcttgcccgt ttgtggcaac ggcctctggg
     1081 ccgccccggg cgagtgcagc cgcgcatcgt cctcccgatc aacacttctt gcagatagtg
     1141 gacatgaacg aacggacttt gcgcaggctt ggtagccggc tggcatggct ggcaccgatg
     1201 ataacgctgg gcggctgctc ctgggatctg ctcgatcccc agggcaacat cggtgtccag
     1261 aatctttcca cccttacctg ggcgacgctg ctcatgctga tcgtggtgat cccggtgatc
     1321 attctgacga tcgcattcgc gtggcgctac cgctcgtcca acctcgcggc tgactatgcg
     1381 cccgactggt cgcactcgac caagatcgag atcgtggtct ggctggtgcc ggcgctgatc
     1441 gtcgcctcgc tggcggtgat cgcctggaag accacgcacg atctcgatcc ctaccgcccg
     1501 atcgcctcca aggagaagcc gatcgaggtc gaggtcgtct cgctcgattg gaagtggctc
     1561 ttcatctacc ccgaatatgg cgtcgcctcg gtcaacgaga tggcgctgcc ggtcggccgg
     1621 ccggtgcatt tcaagatcac gtccgacacg gtgatgaata gcttcttcat cccgcagctc
     1681 ggcagccaga tctattcgat ggcgggcatg cagacccagc tgaacctcgt cgccgacaag
     1741 gccggcagct ataagggcat ctcggccaac ttcagcggca acggctttgc cgacatgcgc
     1801 ttcgaagcca aggcgatgga ccagaagggc ttcgacgctt ggatcgctgc tgccaaggcg
     1861 agcccggcca gcctcgacat ggccggctat gccgcgctcg agaaggccag cgagcatgat
     1921 ccggtccgtt tctacggcgc ggtgacgccc aagctgttcg acgccgtgct gatgaaggac
     1981 atgcacgaca atgcacctgc agagggcaag gcccactcgc agataacctc catgcagatg
     2041 ccggccccgg cgcagcagga ttgatcccca tggatatgag tattctcttc ggcaggctga
     2101 cgcttgacgc catccccctc cacgaaccga tcatcatggg cgctaccgcg ttcatggggc
     2161 tggtcggcct cggcgtggtc tatgcgatcc acagcaaccg cttctgggta ccgctgtgga
     2221 aggattggct gtgctcggtc gatcacaaga agatcggcat catgtacatc atcctcgcgc
     2281 tggtgatgct gctgcgcggc ttctcggacg cgatcatgat gcgcagccag caggcgatgg
     2341 ccgctggcgg cacgcagggc tacctgccgc ctgagcattt cgaccaggtg ttcaccgcgc
     2401 acggcgtcat catgatcttc ttcatggcga tgcctttcgt gattggcctg atgaacgtgg
     2461 tcgtgccgct ccagatcggt gcgcgcgacg tggcgttccc gttcctgaac tcgctgagct
     2521 tctggctcac ggtgtcgggt gcgctgctga tcaacgcgtc gctcgcgatc ggcagcttcg
     2581 cgcgcacggg ctggctggcc tatccgccgc tgtccgagct cgcctactcg cccgacgtcg
     2641 gcgtcgacta ttatatctgg gcgctgcaga tctcgggtgt cggcaccacg ctcgcgggtg
     2701 tcaatttcat cgccaccatc ctcaagatgc gcgcgccggg catggacctg atgaagatgc
     2761 cgatcttcac ctggaccacg ctctgcacca acatgctgat cgtcgccgcc ttcccggtgc
     2821 tgaccggcac gctcgcgctg ctgtcgctcg accgttacct cggcatgcac ttcttcacga
     2881 acgatcatgg cggcaacccc atgaactacg tgaacctgat ctggatgtgg gggcaccctg
     2941 aggtctacat cctcatgatc ccgctgttcg gtgccttctc ggagatcatt gcgaccttct
     3001 cgggcaagcc gctgttcggc tacaagtcga tggtctacgc gaccatgggt atcgccggct
     3061 tgagcttcct cgtgtggctg caccacttct tcacgatggg ttcgggcgcc aacgtcaaca
     3121 ccttcttcgg catcaccacg atgatcatct cggtgccgac gggcgtgaag atcttcaact
     3181 ggctgttcac catgtacaag ggccgcgtcc gcatgaccgt gcccgtgatg tggacaatcg
     3241 gcttcatgct gaccttcacc atcggcggca tgaccggtgt gatgctggcg cttccgggcg
     3301 cggacttcgt gctccacaac tcgctgttcc tgatcgcgca cttccacaac gtgatcatcg
     3361 gcggcgtggt gttcggctgc ttcgcggcga tgaactactg gttcccgaag gccttcggct
     3421 tcacgctcaa cgagacgctg ggcaaggctg ccttctggtg ctggttcatc ggcttctacc
     3481 tggcgttcat gcccctctac gcgctgggct tcatgggtgc gacgcgccgt ctgcagcatt
     3541 atgcggacct cgcctggcag ccttacttca tcgtcgccgc ctgcggcgcc gcggtgatcc
     3601 tgctcggcat cgtcttccag gtggcgcagc tcgtcgtctc gatccgcgac cgcgagcaga
     3661 atcgcgacct gaccggcgat ccgtggaacg gccgcacgct ggagtgggcg acctcgtcgc
     3721 cgccgccctt ctacaacttc gccaagaccc ccaaggtcga ggctctcgat gcctggcacg
     3781 acatgaagaa gaagggcatc tcgccgcttc ccgacacgtt cgcccccatc cacatgccgc
     3841 gcaacacctg ggcgggcttc gtgatcggca tcgcgggcgt gccgctgggc ttcgcgctga
     3901 tctggcacat ctggtggctg gccggcgtga gcttcatcgg catggtcggc gcggccattg
     3961 tccactcgtt caacgaagat cgcgactatt acgtccccgc cgatgaagtg gcggagatcg
     4021 aaggtcgcat cgcgctcgcc aaggttgcct gacatgaaca gcatcaccat gggggtgccc
     4081 catcacgggc accacgacga ccatgagcat cacgaccagt tcgggacggt tcagctcggt
     4141 ttctggatct acctgatgac ggactgcgtg ctgttctgca cgctgttcgc cacctacgcc
     4201 gtgatgtcga ccgcgttcga cggcggcccg aacccgcaca ccatcttcga gctgccctat
     4261 gtggcggtgg agacggcgct gctgctgttc agctcgctga cctacggcat cgcgatgatc
     4321 ggcgcgcacc agggcaagcg cggcatgacg atgggctggc tcctcgtcac cgcgatgttc
     4381 ggcctgggct tcctcggcat ggagatccac gaattctcca agctgatcgc cgagggcgcg
     4441 ggcccggatc ggtcgggctg gctgtcgtcc ttcttcacgc tggtcggcac gcacggtctg
     4501 cacgtgacct cgggtctggt gtggatgacg gtgatgctcg ttcagctcgc caagagcggc
     4561 ctcaacgaga ccacgatccg ccgactgacc tgcctcagcc tgttctggca cttcctcgac
     4621 atcgtctgga tcggggtgtt cagcttcgtc tatctgatgg gagtttcggc atgagcgcgc
     4681 attcgcactc tgacgcacat gcgtcgcacg gcgggctgaa gccctacgcg atcggtttcg
     4741 tgctggcggt gctgctcacc gcgctgccct tcggggcggt gatgaagggc atggtgtcgg
     4801 gcgtgcaggc ggtcgaactc atcatgggca cggccgtggt gcagatcctc gtgcacctgc
     4861 acttcttcct ccacatcgat cgcaagaccg atcgctggac gctgcaggcg ctgttcttca
     4921 cgatcttcgt gctgggcgtg attgttagcg gctcgctgtg ggtgatgttc cacctcaacc
     4981 acaacatgat gccgatgatg ccgatcgacg gctgatcgcc ccgatcgacg catataaaaa
     5041 agggcctcgc tccaacccgg agcggggccc tttctctttt gcgatcaggc gatggtaggc
     5101 agaatgccgc cctcggcccg gatcgcggcg ccgttggtgg cggcggcgag cgggctcgac
     5161 aggaaggcca ccatgttgcc gatctcctca gcctcgatca ggcggcggat cagcgactgc
     5221 gggcgcagct cctcgaagaa cagccgttcg acctcgtcct gcggcgcgtc gcccctggag
     5281 acggtggagc gcatgaagtc gacgatgcct tccgagcggg tcggccccgg cagcaccgag
     5341 ttcaccgtca cgccggtgcc tgtggtctgg gcggcgaggc cgcgcgaaat cgagagctgg
     5401 gcggtcttgg tcatgccgta gtggatcatc tcgccgggga tcatcagcga gctttccgac
     5461 gagatgaaga tcacgcggcc ccagttcttc tcgagcatct tcgggaaata atggcgggcg
     5521 aggcgggcgc cagagaccac gttgacctcg aagaagtgat gccagtcggc gtccgaaatg
     5581 tcggtgaagg ccttggcttc gtagatgccg agattgttga cgaggatgtc cacctcgggc
     5641 gtggcggcga tcagcgtcgc ggcaccctct gcggtcgccg gatcggcgag gacggcgttc
     5701 accttgccga gcttggcgat gcgggcggcg gcttcatcga gcttggcctg attgcggccg
     5761 gcgatggtga cttcaacgcc ctcggcggcg agccgctcgg cgatggcaag gccgatgcca
     5821 gcggtcgatc cggtgacgag tgcggtcttg cccttcaatt gcagatccat gcgggaactc
     5881 ctggttgatg ccgccgccga acatgggagg gagg
//
LOCUS       DATGEM010000026         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_902882, whole genome shotgun sequence.
ACCESSION   DATGEM010000026 DATGEM010000000
VERSION     DATGEM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902882"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4466)
                     /locus_tag="VKQ27_00700"
     CDS             complement(<1..4466)
                     /locus_tag="VKQ27_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87477.1"
                     /translation="MSERNVASGRASRVGRLKLSTALGGGAAAILLLAPAVSAAPVSP
                     YRIVAPTLPGATAKAAAPADSGLDFVNAPQDLTISSPVRATASVGDVVITDGDITTTG
                     DFQPGITSTSTGSTTITAGNITTSGKHSDGVDASGPGPIAITVGNVSTSGNDSAGIHA
                     AGGGDIAVTTGDVTTTGGRSSGIYAHSSGGNVTVNAGNVYAVGSVQSWGIDASGHDVT
                     ITAGNVKGGAIGISARGYGQDAAVDLTVTGTVSSYSFTGHGISAISDGSVTIHDSGSI
                     FSTSITSIGFGEGIYAHGGGDVLVDSSGRIKTYGRQIGIAGFSDSGNVTIDASVVDAY
                     GSRSIGIEAFAAGKATIVADDIVMANNQSGGIVAGGASVDVTLNGMIDAKGQSTNGII
                     AIAYSGDAAIHNNGAITVGNLAGYGIQVTAAGNITIDGKGSVATGRYHGYGIVAFTAG
                     GSISITQGDITANNYGVMATAQTQYYQKGDPSRGITIDVGNVKTTGTGVTATGIVAND
                     RNVYSTVAINAASVSTIGDYAAGIRANAAGGTVTVTGGSITTRGNHAAGVSLIGNDYD
                     SVTLNSITTTGSYHSSGIEIQTNPFHYGTITVDVGSISTGGYLSHGLVALSSGSADIK
                     VGSITTTGTRGFGIYALGSTDVSINVGSIATTGEGSTALIGLSRGGTLSISESSSITT
                     QGDNALAVIAETGGNGAIDLTLDGAISTKGKASAGVVVVAGSGAIQIKAGSISTAGVG
                     APALAAYSFYGSANVDIGSASTGNVKSPAVLVVAGTDATVTAGQLSTSGDNSNALVIR
                     AGSYGVGGEGVVNVNTIVTSGRQSAGIDALAQGDAKHDASISLTAGSITTSGIDSPGI
                     SAFADYGALNIQAGSIKTTGYESTGIMAFGGKDIGIQVGSVDTAATSIYASGSGTGTI
                     TLGVTGKVASSGGHGAVLINSGGATNVTVASTGSIAGAGDALQIQTGTGQVTITNAGT
                     ITGGSGYAIDVSGLPAPMLAFAKLGQASVAAATPGAQIVNSGTIVGAVSLTGGDDVLT
                     NSGTFVATKDSDFGAGNDLFVNSGKLVVQPGTKPGNITLLGLETFQNSGLVDLRNGVA
                     GDTLTLPGNYIASGKAALGLDLGANGIADKLIVNGTVTGTTSILINATAGNATLLATP
                     VTLVQAGGTSMAANAFSIANQSVGFVTYGLSYNAATNSFQLTAQAGAPVYRFAKVNEA
                     AQAIGQQVGTAWRSHMAELRDMDEPARRVWGQAYGQVDSRHEDRTVAVAGSTAQNYDL
                     GYRQDYFGAQAGVDLGGKRAENGNGLLFGITGGYLSSHMNFRAGADRVRFDTVNAGGY
                     AGVRAGRLFANLLGQYVHYRTTAFNGVEQWSDKFSGNGYGVQGEVGARLGSDTLFAEP
                     VASLAWQKTDLGTLNALGQSLDFGHDSGLTGSFGARFGGSVAMAGGGKAVFYAKASYV
                     HAFSGKGGVLFTSGGTSEDISGTKLGDYGQAAVGADFLTTGRVSGFIEANADV"
     gene            complement(4549..>6801)
                     /locus_tag="VKQ27_00705"
     CDS             complement(4549..>6801)
                     /locus_tag="VKQ27_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY87478.1"
                     /translation="AGLDLPAGPLSQAIAALAERYGVSIGAADSRLLAIPLPPLHLRG
                     SPAEMLARIDRVAKVSAVRLAPGVWRIVRLPPVPRPITPPSPVMTTIIVEASKRRERL
                     EDDPVEIVSLRGSDLGRYALSPDTGALSGLVPILISTDWGAGRNKLFLRGIADSSFTG
                     SSPALVGEYLGDQRLTYSAPDPDLRLYDVSRVEVLAGPQGTLYGAGSLGGLIRIEPNS
                     PLPGQFGGAGWLGLSATAHGGVGGDFGGVINVPLAGDRLALRAVGYAASDAGYIDDPG
                     RGLKNINRTRTRGGRLALRWAMGDGWSVDLIGVAQRIDNRDAPYADPDGPPLTHFSMI
                     AQPSYNLFLSGSVVVAGHIGGLSVRSTTGIVHQSLGERFAALRPGAIINLFDEREHPS
                     LLSQETRISGASDHANWVLGAAYLSNINSEARDYGRPDGPTPIARLRERMREFTVYGQ
                     VTRRLVGPLSATIGGRYAHDRLSGETFDNAMPFIRLLAPNIRAVSTEHHLVPSVSLSY
                     AMSGGAIVFLRYETGFRPGGLTIASTAQRYAGDHIATTELGVRRGASGDDRFAFSLTA
                     AISRWHDIQADILDGIGMPKIANIGDGRVDSLDATCDVRIMQGLRLGLAGFVARSRLS
                     PTEDLAAGGDATTLPNVAKNGGTVTLDYRHDDSRNRSWQAGVRVQHVGPSVLGVGPAL
                     ARRQSDYTTMALDAGVQILGTELSLGITNLLDSRKYVFAVGTPFAALIERDVTPLRPR
                     TVRLALGYKF"
BASE COUNT         1228 a         2236 c         2143 g         1194 t
ORIGIN      
        1 acatcggcat tggcctcgat gaagcccgac acccggccgg tggtcaggaa gtcggcgccg
       61 accgccgcct ggccatagtc gccaagcttg gtgccggaga tatcctcgct ggtgccgccg
      121 ctggtgaaca gcaccccgcc cttgccggag aaggcgtgga catagctcgc cttggcatag
      181 aacaccgcct tgccgccgcc ggccatcgcc acgctgccgc cgaagcgcgc cccgaagctg
      241 ccggtgagcc cgctgtcatg cccgaagtcg aggctctggc cgagcgcgtt gagcgtgccg
      301 agatcggtct tctgccaggc caggctcgcc accggctcgg cgaacagcgt gtcgctgccc
      361 agccgtgccc cgacttcgcc ctgaacccca tagccgttgc cgctgaactt gtccgaccat
      421 tgctcgaccc cgttgaaggc ggtggtgcgg taatggacat actgaccgag caggttggcg
      481 aacagccgac cggcgcggac ccccgcatag cccccggcat tgaccgtgtc gaagcgcacc
      541 cggtcggcgc cggcgcggaa gttcatatgg ctcgacagat agccgccggt gataccgaac
      601 agcaggccat tgccattctc ggcgcgcttg ccgcccaagt cgacaccggc ctgggcgcca
      661 aaataatcct ggcgatagcc gagatcatag ttctgcgccg tgctgccggc gaccgcgacg
      721 gtgcgatcct catggcggct gtcgacctgg ccataggcct gcccccacac ccggcgggcg
      781 ggctcgtcca tgtcgcgcag ctcggccatg tggctgcgcc acgccgtgcc cacctgctgg
      841 ccgatcgcct gcgccgcctc gttgaccttg gcgaagcgat agaccggcgc gccggcttgc
      901 gcggtgagct ggaagctgtt ggtcgccgcg ttgtacgaga ggccataagt aacgaagccg
      961 accgactggt tggcgatcga gaaggcgttg gccgccatgc tcgtgccgcc cgcctgaacc
     1021 agcgtcaccg gcgtcgcgag cagcgtcgca ttgccggccg tcgcgttgat caggatcgag
     1081 gtggtgccgg tgacggtgcc attgacgatg agcttgtcgg cgatgccatt ggcaccgagg
     1141 tcgagaccca gcgccgcctt gcccgaagcg atataattgc ccggcaaggt cagtgtgtcg
     1201 ccggcaaccc cgttcctgag atcgacgagg ccactgttct ggaaggtctc gagaccgagc
     1261 agcgtgatgt tacccggctt ggtgcccggc tgcaccacca gcttgcccga gttcacgaac
     1321 agatcgttgc cagcgccaaa gtcgctgtcc ttggtcgcga cgaacgtgcc cgagttggtc
     1381 agcacatcgt cgccaccggt cagcgagacc gcaccgacaa tggtgccgct attgacgatc
     1441 tgcgcacccg gcgtcgccgc cgcgacgctg gcctggccaa gcttggcgaa agccagcatt
     1501 ggcgcgggca gaccactcac gtcgatcgca taaccgctgc cgccggtgat cgtaccggcg
     1561 ttggtgatcg tgacctgccc ggtgccggtc tggatctgca gcgcgtcacc ggcgcccgcg
     1621 atgctgccgg tcgaagccac ggtgacgttg gtagcgccac cgctgttgat cagcaccgcc
     1681 ccatgaccgc ccgacgacgc caccttgccc gtcacgccca gggtgatcgt gcccgtaccg
     1741 cttccggatg cgtaaatcga cgtggctgcg gtatcgaccg acccgacctg aatgccgatg
     1801 tccttaccgc cgaaggccat aatgcccgtc gactcatagc cagtcgtctt gatcgagccg
     1861 gcctggatat tcagggcgcc ataatctgcg aaggccgaaa tgccggggct atcgataccg
     1921 ctcgtcgtga tggaacccgc cgtcagcgaa atcgatgcgt cgtgcttcgc gtcgccctgg
     1981 gcaagcgcat cgatacccgc gctctgtcgg ccactggtca cgatggtgtt gacgttgacc
     2041 acgccctcac cgccgacccc gtagctgccc gctcgaataa caagtgcgtt ggagttgtcg
     2101 ccactcgtcg aaagttgccc ggctgtgacc gtggcatcgg tgccggcaac gaccaacaca
     2161 gccggcgatt tgacgttgcc cgtgctcgcc gagccgatat ccacgttcgc cgatccgtag
     2221 aaggaatagg cggccaatgc cggggcaccg acgccggcag tgctgattga gcccgccttt
     2281 atctggatcg cgccagatcc ggccacgaca acgacgccag ccgatgcctt gcctttggtc
     2341 gagattgcac cgtcgagtgt caggtcaatt gcgccgttcc cgccggtctc ggcgattaca
     2401 gccagtgcgt tgtcgccttg agtagtgatc gagctgctct cggatatgct cagcgtgccg
     2461 ccgcgcgaca acccgatcag cgcggtcgag ccctcgccgg tcgtcgcgat cgagccaacg
     2521 ttgatggaga catccgtgct gcccagggca tagatgccga agccgcgcgt gcccgtcgtg
     2581 gtgatgctgc ccaccttgat gtcagctgac ccactcgaga gcgctaccag cccatgcgag
     2641 agatatccgc cggtcgagat cgacccaacg tccaccgtga tcgtaccgta gtggaacggg
     2701 ttggtttgga tctcgatgcc gctggaatga taggagcctg tcgtggtgat cgaattcagc
     2761 gtgacgctgt cataatcatt gccgatcagc gacacacccg cagcatgatt gccgcgggtc
     2821 gtgatgctgc cgccggttac cgtgacagtc ccgccggctg catttgccct gatcccggcg
     2881 gcatagtcgc cgatcgtgga cactgaagcc gcgttgatcg cgaccgtgct gtacacgtta
     2941 cgatcgttcg cgacgatgcc ggtcgcggtg acgccggtgc ccgtcgtctt cacattgccg
     3001 acatcgatcg tgatgccgcg gctgggatca cctttttggt agtactgggt ctgggcggtc
     3061 gccatcacgc cataattgtt cgccgtgata tcaccctgcg tgatcgagat ggatccgccc
     3121 gcggtgaagg cgacgatgcc ataaccatga taacgacccg tcgcgaccga tcctttgcca
     3181 tcgatggtga tattgcctgc ggcggtcacc tggataccgt agccggcaag attgccgacc
     3241 gtgatcgcgc cgttgttgtg gattgccgcg tcgcctgaat aggcgatggc aatgattccg
     3301 ttggtgcttt gccccttggc atctatcatg ccgttcagcg tgacgtcgac cgacgctccc
     3361 cccgcaacga tacctcccga ttgattgttt gccatgacga tgtcgtcggc cacgatggtc
     3421 gctttgccag cagcaaaagc ctcgatgccg atcgagcggc tcccgtaggc gtcgacgacg
     3481 ctggcatcga tggtgacgtt gccgctatcg ctgaacccgg cgatcccgat ctggcgaccg
     3541 taggttttga tccgaccaga gctgtcgacc agaacgtcgc cgccgccatg ggcgtaaatg
     3601 ccttcgccga agcctatgct ggtgatgctc gtgctgaaaa tcgaaccgga atcgtgaatc
     3661 gtcaccgacc catcggagat ggcgctgatg ccgtgcccgg tgaagctgta ggacgaaacg
     3721 gtgccagtga cggtcaggtc aacggctgca tcctgaccat agccccttgc agaaatcccg
     3781 atcgcaccac ccttcacgtt gccggcggta atggtgacat catgcccact cgcatcgatc
     3841 ccccacgact gcacgctgcc gacggcgtaa acgttgcccg cattgacggt aacgttgccg
     3901 ccgctggaat gggcgtatat tccggaggaa cgaccaccgg tcgtggtgac atcaccggtc
     3961 gttacggcga tgtccccgcc gcctgcggcg tggatgccgg cagaatcatt tccgctcgtg
     4021 ctgacattgc cgaccgtgat cgcaatcggt ccggggccgc tcgcgtccac gccatccgaa
     4081 tgcttgccgc tcgtggtgat gttgccagca gtgatcgtcg tgctgccggt tgacgtagag
     4141 gtaataccgg gctggaaatc gcccgtggtc gtgatgtccc cgtcggtgat gaccacgtcg
     4201 cccaccgagg cggtcgcccg caccggactg ctgatcgtga gatcctgggg agcattcacg
     4261 aagtcgaggc cactgtcggc cggcgcggcc gcctttgccg tcgctccggg cagagtcggg
     4321 gccacgatac gatagggcga caccggagcg gccgacaccg caggtgcgag caacaggatc
     4381 gctgccgcgc ccccgccaag ggcggtgctc aatttcagcc ggccaacccg cgacgcccga
     4441 cccgatgcaa cgttccgctc actcatcaaa ccgcccctaa aatatcgttg tccgaaggaa
     4501 ggacaccggg gggggccagg agccgtaatc gctacgacgg aaatttttct aaaatttgta
     4561 acccagtgcc aaacgcaccg ttcgaggacg gagcggggtg acgtcgcgct cgatcagcgc
     4621 cgcgaacggg gtgcccacgg cgaagacata ttttcggctg tcgagcaggt tggtgatgcc
     4681 gagcgacagt tccgtaccga gaatctgcac ccccgcatcg agcgccatcg ttgtatagtc
     4741 gctttgccgc cgagcgagcg ccggcccaac tccaagaacc gaagggccga catgctggac
     4801 gcgcacgcct gcctgccacg accgattccg cgaatcgtcg tgccgatagt cgagcgtcac
     4861 cgtgccgccg ttcttcgcga cattgggcag ggtcgtcgca tcgccacccg ccgcgagatc
     4921 ctcggtcggg ctcagcctgc tgcgggcgac gaagccggcc aggcccagcc gcaaaccctg
     4981 catgatgcgc acatcgcagg tcgcatcgag gctatcgacc ctgccgtcgc caatgttggc
     5041 gatcttgggc atgccgatcc catcgaggat atcggcctgg atgtcgtgcc accggctgat
     5101 ggccgcggtg agcgaaaaag cgaaacgatc gtcgcccgag gcaccacgcc gaacgcccag
     5161 ttccgtcgtc gcgatgtgat cgccggcgta acgctgggcg gtgctggcga ttgtcagtcc
     5221 acccgggcgg aagccggttt catagcgcag gaagacgatc gcgcccccgc tcatcgcata
     5281 agacagcgaa accgaaggca cgagatgatg ttcagtgcta accgcgcgga tgttcggagc
     5341 cagaagccga atgaacggca ttgcattatc gaaggtttcg ccagagagcc gatcatgcgc
     5401 atagcgcccg ccgatcgtgg ccgagagcgg cccgaccaga cgccgtgtca cctgaccgta
     5461 gaccgtaaac tcgcgcatcc gctccctcaa tcgagcgatg ggcgtcggcc catccggccg
     5521 gccataatcg cgtgcctcgc tgttgatatt gctgagatag gctgcaccca atacccagtt
     5581 ggcatgatcc gaagcccctg aaatccgggt ttcctgggac aacaggctcg gatgttccct
     5641 ttcgtcgaac aggttgatga tcgcccccgg acgcagcgcc gcgaagcgct cgccgagcga
     5701 ctgatggacg atcccggtgg ttgaccggac ggagagccca ccgatgtgcc ccgcaacgac
     5761 cacgctgccc gacaggaaca ggttgtagga tggttgcgcg atcatgctga agtgggtgag
     5821 cggcggcccg tcaggatcag catagggcgc gtcgcgattg tcgatccgtt gcgcgacacc
     5881 gatcaggtcg acgctccagc cgtctcccat cgcccaacgc aaggcgagtc ggccgccgcg
     5941 agtccgggtt cggttgatgt ttttcagtcc acggccagga tcgtcgatat agccggcgtc
     6001 actggccgcg tagccgaccg cccgcagcgc aagtcgatcg ccagcgagcg gaacgttgat
     6061 cacgccacca aaatctccgc cgacacctcc gtgcgcggta gcggacaggc ccagccaccc
     6121 ggcaccgccg aattggcccg gcaacgggga attaggctcg atacggatca gtccgccaag
     6181 cgaaccggcg ccatagagcg tgccctgcgg cccagcgaga acctctacgc gcgacacatc
     6241 gtaaaggcgc agatcgggat cgggcgcgct ataggtcaag cgttgatcgc caagatattc
     6301 gccgacaagt gcagggctcg aaccggtgaa gctgctgtcg gcgataccac gcaggaacag
     6361 cttgttgcgg ccggctcccc agtcggtcga aatcagaatg ggcaccaggc cggacagcgc
     6421 tccggtatcc ggcgacaatg cgtaacggcc gaggtcgctc ccccggaggc tgacgatttc
     6481 tacggggtcg tcctccagcc gctcgcgccg cttggaagcc tcgacgatga tcgtggtcat
     6541 gaccgggctc ggcggcgtga tcgggcgcgg cacaggcgga aggcggacga tccgccacac
     6601 gccgggagca aggcggaccg cgctaacctt ggccacacgg tcgatgcggg ccagcatctc
     6661 ggccggagag ccgcgaagat ggagcggcgg cagcgggatc gccagcaggc gagaatcggc
     6721 ggcgccgatg ctgaccccgt atcgctcggc cagcgctgcg atagcttgcg acaaagggcc
     6781 cgcgggcaga tcgagcccgg c
//
LOCUS       DATGEM010000027         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1017850, whole genome shotgun sequence.
ACCESSION   DATGEM010000027 DATGEM010000000
VERSION     DATGEM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017850"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="VKQ27_00710"
     CDS             complement(<1..1362)
                     /locus_tag="VKQ27_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLY87479.1"
                     /translation="MSDYEVVETDVLVVGAGGAGLRAAIAAAESGARVLVICKSLLGK
                     AHTVMAEGGVAAAMHNVAYQDSWEAHFADTMKGGKLINDWHMAEIHAREAPERVLELE
                     RWGAVFDRTWQGRIHQRPFGAHTYPRLAHIGDRTGLELLRTLQDRAVHTPGMGVHMEV
                     TVFKLLKSEGHIAGALAYRRSDGSLILYRCGAVLLATGGAGKMYRITSNSWECTGDGS
                     ALAYDAGAQLRDMEMVQFHPTGMVWPAGVRGILVTEGVRGEGGVLRNKEGERFMERYD
                     HERMELSSRDVVARAINSEVAAGRGSPHGGVFLDITHRKPDFIKNKLPSMYEQFLKLA
                     NVDITKQPMEVAPTIHYAMGGVRVEAETCAASVPGLFAAGEVASGLHGANRLGGNSLS
                     DLLVFGKRAGEAAAEAARKGAKPKSVDERDIDDAIGVLMAPLTRAGGESPYKIMAEIQ
                     DVMT"
     gene            complement(1359..2120)
                     /locus_tag="VKQ27_00715"
     CDS             complement(1359..2120)
                     /locus_tag="VKQ27_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87480.1"
                     /translation="MALSQPVTRPAKARQAVRNPYSVLPNWLYPTFVVGALSAFAVYA
                     VWIVFFQPAGYFAPYLSPFDSPLVKVGPIPPGVWVAWAPFVFRLTCYYYRKAYFRSFF
                     GHPRSCATPERRRNRYRGETVFPWVLNNFHRFAFYATVVQVAFLWFDAIIALDFGGRF
                     GIGIGSLLMVINVALLSAYTFGCHAFRHLAGGGLDCYSCSAVSRARFRLWRGITVLNV
                     RHDRWAWASLASVLLTDIYVRLLMNHALIDVRWIS"
     gene            complement(2194..2790)
                     /locus_tag="VKQ27_00720"
     CDS             complement(2194..2790)
                     /locus_tag="VKQ27_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87481.1"
                     /translation="MEAALVVVFAAAAGFMFYQGSFITQQVNDELVSQKISFPAATEI
                     KTGGALDPAVFPQEIRDLAGQQVDNGYRARIYAQDFIRIHEQKIANGETYSTMAGKIA
                     AVNAQIAATPKTDPAYADLQKQLATMNGQRDTLFKGDMLRGALLNSYGWWTFGTYMVY
                     GALAALAAAVLALGALTFEVLFAARKPKTVKIAQRATA"
     gene            3038..>3388
                     /locus_tag="VKQ27_00725"
     CDS             3038..>3388
                     /locus_tag="VKQ27_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLY87482.1"
                     /translation="MSAPVISIAPEATIKAAARLLVSCGISALPVIDAKGALVGIVSE
                     ADLIPIETRPDPRSQATPLSPAAGTAPRIVADVMTRNVVTVPTDCEVSQAARLMIEAE
                     VKRLPVMDGQRLAGI"
BASE COUNT          601 a         1093 c         1085 g          609 t
ORIGIN      
        1 cgtcatcacg tcctggatct cggccatgat cttgtacggg ctctcgccgc ccgctcgggt
       61 cagcggcgcc atgagaacgc cgatcgcatc gtcgatgtca cgctcgtcga cgctcttcgg
      121 cttcgcgccc ttcctggcgg cctcggccgc ggcctcgccc gcgcgcttgc cgaacacgag
      181 caggtcgctc agcgagttgc cccccaggcg attggcgccg tgaaggccgg acgctacctc
      241 gccggcggcg aacagccccg gcacgctcgc cgcgcatgtc tccgcctcca cgcgcacgcc
      301 gcccatcgcg tagtggatcg tgggcgcgac ctccatcggc tgcttggtga tgtccacgtt
      361 ggcgagcttc aggaactgct cgtacatcga cggcagcttg ttcttgatga agtcgggttt
      421 gcggtgcgtg atgtccagga acacgccgcc gtggggcgag ccgcgcccgg cggccacctc
      481 ggagttgatc gcgcgggcga cgacgtcacg cgacgaaagc tccatccgct cgtggtcgta
      541 gcgctccatg aagcgctcgc cctccttgtt gcgaagcacg ccgccctctc cgcgcacgcc
      601 ttcggtgacg aggatgccgc ggacacccgc cggccacacc atgccggtgg ggtggaactg
      661 gaccatctcc atgtcgcgca gctgcgcacc ggcgtcataa gcgagcgccg atccgtctcc
      721 cgtgcactcc caggagttgg aggtgatgcg atacatcttg ccggcgcctc ccgtggcgag
      781 gagcacagca ccacatcggt agaggatcag cgatccgtcg gatcgccgat aagcgagcgc
      841 accggcgata tggccctcgg atttcaagag cttgaacacg gtgacttcca tgtggacacc
      901 catgccaggc gtgtgcacgg cgcggtcttg cagcgtccgc agcagctcga gcccggtgcg
      961 gtcgccgata tgcgccaggc ggggatacgt gtgtgcgccg aacggtcgct gatggatgcg
     1021 gccctgccac gtgcgatcga agacggcacc ccagcgttcc aactccagaa cccgctcggg
     1081 cgcttccctg gcgtgaatct cggccatatg ccagtcgttg atcagcttgc ctcccttcat
     1141 ggtgtctgcg aaatgggcct cccagctgtc ctggtaggcg acgttgtgca tcgccgccgc
     1201 aacgcccccc tcggccatca ccgtgtgcgc cttaccaagc agcgatttgc agatgaccag
     1261 cacgcgagcg ccggattcgg ccgccgcgat cgcggctcgc aggccggcgc cgcctgcacc
     1321 gacgaccagc acgtccgttt caacgacctc gtaatcgctc aagagatcca cctcacgtcg
     1381 atgagggcgt gattcatcag cagcctgacg tagatgtcgg tgagcaggac cgaggcaagg
     1441 cttgcccagg cccagcggtc gtggcggacg ttcaggaccg tgatgcccct ccacaatcga
     1501 aatctcgcgc gcgaaacggc tgagcacgaa tagcagtcga gtccgcctcc cgcgaggtga
     1561 cggaaggcgt ggcacccaaa cgtgtaagcg gagagcagcg caacgttgat gaccatcagc
     1621 aggctcccga tcccgattcc gaaccggccg ccgaaatcca aagcgatgat cgcgtcgaac
     1681 cacagaaatg caacctgcac gaccgtggcg tagaaggcga aacgatggaa gttgttcaag
     1741 acccagggaa agacggtctc gccgcgatat cggttgcgcc ttctttctgg agtcgcgcac
     1801 gaccggggat gcccgaagaa ggagcggaag taggccttgc ggtagtagta gcaggtcagc
     1861 cggaagacga aaggcgccca ggcgacccag acgccgggtg ggattggtcc aaccttcacc
     1921 agcggcgagt caaacgggct gaggtaaggg gcgaagtagc cggcaggctg gaagaagacg
     1981 atccagacgg catagaccgc gaaggcgctg agcgctccga ccacgaaggt cggatacagc
     2041 cagttcggca ggacgctgta gggattgcgg acggcctgcc gggctttggc aggccgggtc
     2101 accggttggg aaagcgccat ctgagcctgc taatcctcca tcaaaaaaag agctccggca
     2161 gatcgatcct gccggagcat gccgtccgta ctttcacgca gtagcgcgct gcgcgatctt
     2221 gactgtcttg ggtttccggg ctgcgaagag gacctcgaac gtcagggcgc cgagtgcgag
     2281 caccgcggcc gcgagggccg ccagcgcacc gtagaccatg taggttccga acgtccacca
     2341 gccgtagctg ttcagcaatg ctccgcgaag catgtcgccc ttgaagagcg tgtcccgctg
     2401 accgttcatg gtcgcgagct gcttctgaag atccgcgtag gcgggatcag tctttggtgt
     2461 ggcagcgatc tgggcgttta ccgccgcgat ctttccggcc atggtcgagt aggtctcacc
     2521 gttggcgatt ttctgctcgt gaatccgaat gaagtcctgc gcgtagatcc tggccctata
     2581 gccgttgtcg acctgctggc ccgcgaggtc gcggatctcc tgcgggaaga cagcggggtc
     2641 gagcgcaccg ccggtcttga tctcggtcgc ggctgggaac gagatcttct gtgacaccag
     2701 ttcgtcgttg acctgctggg tgatgaagct gccttggtag aacatgaagc ccgctgctgc
     2761 ggcgaaaacc accaccaggg cggcctccac aatcgcgatc ggtccgaaaa ttcctctttt
     2821 cacgttacgt ctccttcggg ttccattccc tgatggggat ttgatttcag ttgagccccg
     2881 gacggctcat gcgcttagtc aacagctgtt gcgagctcgc cacgaccgcc gttcgaccct
     2941 ttgcgagggg gcgtcggacc cttcctgaaa aggaccattg gatctttctc gcggttgggg
     3001 ccctccgcac gctatgggca tgaaagtcgc ggcggtgatg tcggcgccgg tgatcagcat
     3061 cgctcccgag gcgacgatca aggccgctgc gcgcctgctt gtgtcgtgcg gcatctcggc
     3121 tctgccggtc attgatgcca aaggcgcgct cgtcggcatc gtttcggagg ctgatctgat
     3181 tccgatcgag acgcgccccg accctcgctc gcaggcgacg ccactcagtc ccgcggcggg
     3241 aaccgcgccg cgcatcgtcg cagacgtgat gacccgaaat gtcgtgaccg tgcccaccga
     3301 ctgcgaggtc tcgcaggctg cgcgcctgat gatcgaggcc gaggtcaagc ggctcccagt
     3361 gatggatggt caacggctgg ccggcatt
//
LOCUS       DATGEM010000028         6881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_913099, whole genome shotgun sequence.
ACCESSION   DATGEM010000028 DATGEM010000000
VERSION     DATGEM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913099"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1231)
                     /locus_tag="VKQ27_00730"
     CDS             complement(128..1231)
                     /locus_tag="VKQ27_00730"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HLY87483.1"
                     /translation="MFTRLFALSILTLAAVPLAQAERIKDMAQVQGVRTNQLIGYGLV
                     VGLDGSGDQTSQAPFTVQSLKNMLAQLGVTVPANVNPQLKNVAAVSVHAVMPAFVKPG
                     QTIDITVNSIANAKSLRGGSLLVTPLHGLDGQIYAIAQGNLIVSGFGVEGTDGSKLTV
                     NVPSSGRVPNGATIERAVATQITQGDTITLNLNTPDFTTATRLVESVNRGVGEGSATI
                     VDGGSVLVRAPHDATQRIAFLSMVENLQVDPGSAAARVIINSRTGTVVISSNVTVSPA
                     AVSHGSLSVTIKEEQSVSQPAPFSNGETKVVSNSTIKVDQGSNRMFLFKPGVELEQIV
                     RAVNEVGAAPGDLVAILEALREAGALQAELIVI"
     gene            complement(1265..1969)
                     /gene="flgH"
                     /locus_tag="VKQ27_00735"
     CDS             complement(1265..1969)
                     /gene="flgH"
                     /locus_tag="VKQ27_00735"
                     /inference="COORDINATES: protein motif:HMM:NF001304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HLY87484.1"
                     /translation="MNNLEPGLLSALRFTGGAVLCAMMLAGCGMLPAKERKPEPVVTR
                     ALPPPTPRTDGAIYQAGQQMELFADLKARRIGDILTVKLNESTAASKNAETKTTKATA
                     VANTGPTIFGKTITTKGVPILNTSMTGSDSFDGAGAVTQSNSLAGSLTVTVTDVQANG
                     NLVVQGDKTLHLNQGDEYVHIAGVIRTADIATDNTVTSDKIADAHISYSGKGVVDGTN
                     RMGWLARFFNSPFAPF"
     gene            complement(1969..2499)
                     /locus_tag="VKQ27_00740"
     CDS             complement(1969..2499)
                     /locus_tag="VKQ27_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HLY87485.1"
                     /translation="MLQTQGNLDMSITGLGFFQVTMPDGTIAYTRDGAFSMDSQGNVV
                     NSSGNPISPAITIPANAQSVSVGTDGTVSVTTAGSAKATSVGQIQLASFINVQGLQPV
                     GNNLLTESASSGAPQVGTPGTGGLGNLSQGSLETSNVNTVTELVNMIQCQRAYEMNSK
                     AISTTDQMLQYLTQNV"
     gene            complement(2688..2753)
                     /locus_tag="VKQ27_00745"
                     /pseudo
     CDS             complement(2688..2753)
                     /locus_tag="VKQ27_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2785..3525)
                     /gene="flgF"
                     /locus_tag="VKQ27_00750"
     CDS             complement(2785..3525)
                     /gene="flgF"
                     /locus_tag="VKQ27_00750"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HLY87486.1"
                     /translation="MDRGLYVAMTGAKQIMQAQAVNNHNIANSSTTGFRADGVAFTSE
                     PIYGPGYATRVNAVAGDAGTDFSSGVLMNTGSPLDIAVNGKGFIAVRGADGKEAYTRA
                     GDLQVTALGAVITSTGFPVLSESGPLTVPPSTQVTIGTDGTVSVVPLGLSAAAQSQVD
                     RIKLVNPSTKDLQKGPDGLLRLKSGGQAKTDETVTVVSGELESSNVNAAQSLVNMIEL
                     QRLYELQIKSISSTDQNEQSAQRMMMAA"
     gene            complement(3587..4810)
                     /gene="flgE"
                     /locus_tag="VKQ27_00755"
     CDS             complement(3587..4810)
                     /gene="flgE"
                     /locus_tag="VKQ27_00755"
                     /inference="COORDINATES: protein motif:HMM:NF004238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HLY87487.1"
                     /translation="MAFSTALSGLTAASSDLDVTANNIANANTIGFKDARAQFADVYA
                     AGAVNLNNSVIGQGVRLSSTRQQFTQGNITTSSSNLDLAISGDGFYTLKSANGLVYSR
                     NGQFGEDKSGNVISSTGEALQVYPPLVNGGFNTGTLSNLNLQTAQSAPLATTTGTVIL
                     NLPANTTPPSVAPFNPANSLTYNQSTSTTVYDSLGNAYPATMFFSQQAAPANTWSVDM
                     TVNGVSAGPTQTLTFGSSGQVTAPVGGVLNFNGFTPTSGAQPMSLAFNFGQTTQYGGQ
                     FGVTSITQNGYATGQLSTVAIDPTGIVSAVYTNGRSTQLGQLAMANFPNPQGLKSLGD
                     TNWSETFTSGTVVSGVAGSAGFGSIQSGALESSNVDLTTQLVDMITAQRAFQANAQVI
                     TTANQESQTIINIGH"
     gene            complement(4831..5535)
                     /locus_tag="VKQ27_00760"
     CDS             complement(4831..5535)
                     /locus_tag="VKQ27_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HLY87488.1"
                     /translation="MTSSVLFPNPSYTGATSTSSASGSSGSAPAAGAATLGGTNFLTL
                     MLAQLKNQDPTSPVDSNQFLNQLASLSTVQGITQLNTSFGALSSSLVSSQALQASSLL
                     GHQALVSSKTANLTANGSVSGAIEVPQTTSQAVVNISDSSGALLRQINLGAQSNGLAN
                     FSWDGKLQDGSQAPAGQYTLSAQFNGGASGATAATTYVNGIVQSVTMGAGQTGLTLNV
                     SGLGSVPFSGVKQISN"
     gene            complement(5555..5965)
                     /gene="flgC"
                     /locus_tag="VKQ27_00765"
     CDS             complement(5555..5965)
                     /gene="flgC"
                     /locus_tag="VKQ27_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HLY87489.1"
                     /translation="MFNVAGSALNAETIRLNTTASNLANAESVNGDESKVYRARHPVF
                     QTMMDAGQAGFDGEAAAAGVRVVGVVESKAPSAMRYQPDHPLANKDGYVFMSNVNSIE
                     EMTNMISANRSFATNVECINTARDLLLKTIAMGH"
     gene            complement(6021..6410)
                     /gene="flgB"
                     /locus_tag="VKQ27_00770"
     CDS             complement(6021..6410)
                     /gene="flgB"
                     /locus_tag="VKQ27_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HLY87490.1"
                     /translation="MFDNIFGIHERALLLHGQRIGVLAANMANADTPNFKARDIDFSE
                     VLAGTDASARLPMSTTQASHIALNDAEEGGGELKYRNPFQASLDGNTVEMPVEQAAFS
                     ENNVRYQASLMFINSRIAELQFAIAGQ"
BASE COUNT         1086 a         2071 c         2263 g         1461 t
ORIGIN      
        1 gccgggggtt cgccaaccgt ggcgaacccc gccgtcgcat gcgacggccc tgtttttcac
       61 gatccggaaa acacctgtcc aatccggcct cctctcgagc tttcccgtcg ttcatgaaaa
      121 actcggctta gatcacgatc aattcggcct gcagggctcc ggcctctcgc agagcttcca
      181 ggatggccac caggtcgccg ggcgccgctc ccacctcgtt gacggcacgc acgatctgtt
      241 ccaattcgac gccgggcttg aacaggaaca tgcggttcga gccttgatcg accttgatgg
      301 tcgaattgct cacgaccttc gtttctccgt tggaaaaagg cgccggctga ctgacgcttt
      361 gctcttcttt gatggtgacg gacaaggacc catgggacac ggccgccgga ctgaccgtca
      421 cgttgctgct gatgaccacc gtgccggtcc gcgaattgat gatgacgcgc gccgcggcgc
      481 tgccgggatc cacctgcaag ttctccacca tcgacagaaa ggcgatgcgc tgcgtggcgt
      541 cgtgcggagc gcgcaccagg acggagcccc cgtcgacgat ggtggctgag ccctccccca
      601 cgcctcggtt gacgctctcg accaggcgcg tcgcggtggt aaagtccggc gtgttgagat
      661 tcaaggtgat ggtatcgccc tgcgtgatct gcgtggccac cgcgcgttcg atggtggcgc
      721 cgttcggaac ccggccggaa ctcggtacgt tgacggtgag cttcgagccg tcggtgcctt
      781 ccacgccgaa gccgctgacg atgaggttgc cctgcgcaat ggcatagatc tgaccatcca
      841 gaccgtgcag gggtgtcacc agcagcgagc cgccgcgcag gctcttcgcg ttcgcgatcg
      901 agttcacggt gatgtcgatg gtttgtcccg gcttgacgaa tgccggcatg acggcatgca
      961 cggagaccgc ggcgacgttc ttcagctgcg gattgacgtt cgcgggcacg gtcaccccca
     1021 gctgcgccag catgttcttc agggactgta cggtgaacgg cgcctggctg gtttgatcgc
     1081 cgctgccgtc caagccgacc acgaggccgt agccgatcag ttggttggtg cgtaccccct
     1141 gcacctgcgc catgtccttg atgcgctcgg cctgcgcgag cggcacggct gccaaggtca
     1201 ggatcgatag cgcgaatagt cgggtgaaca tgatgctggg cctcgtgcct ggactttgag
     1261 ccgcttagaa cggcgcgaac ggtgagttga agaaccgcgc gagccagccc atgcgattgg
     1321 tgccgtcgac cactcccttg cccgagtagc tgatgtgggc gtccgcgatc ttgtcggagg
     1381 tcacggtgtt gtcggtggcg atgtcggccg tacgaatcac gcccgcgatg tgcacgtact
     1441 cgtcgccctg gttgaggtgc agggtcttgt caccctgcac caccagattc ccattggcct
     1501 gcacgtcggt cacggtcacg gtcaggctgc ccgccagact gttgctttgc gtcaccgcgc
     1561 cggcaccgtc gaagctgtcg gagccggtca tcgacgtgtt gagtatgggc acacccttgg
     1621 tggtgatggt cttgccgaag atggtgggtc cggtattcgc gaccgccgtc gccttcgtgg
     1681 tcttggtctc cgcgttcttg ctggcggccg tggattcgtt cagcttgacg gtcaggatgt
     1741 cgccgatgcg gcgcgccttc aggtccgcaa agagttccat ctgctgtccg gcctgataga
     1801 tggccccgtc ggtgcgcggc gtgggcggcg gcagcgcccg cgtcaccacc ggttcgggtt
     1861 tgcgttcctt ggccgggagc atgccgcagc ccgccaacat catcgcgcag agaacggcgc
     1921 cgccggtgaa ccgcagagcc gagaggagac cgggttcgag attattcatc atacgttctg
     1981 cgtcaggtac tgcaacatct gatcggtggt gctgatggcc ttcgagttca tttcgtaggc
     2041 ccgctggcat tggatcatgt tgaccagttc cgttactgtg ttcacgttcg atgtttcgag
     2101 cgaaccctgc gaaagatttc cgaggccgcc ggtacccggc gtgcccacct gcggcgcgcc
     2161 gctcgatgcg gattccgtca acaggttgtt gccgacgggc tgcaggccct gcacgttgat
     2221 gaagctcgca agctggatct gacccacgct ggtggccttg gccgaacccg ccgtggtcac
     2281 cgacaccgtg ccgtcggtgc ccaccgagac cgattgcgca ttcgccggga tggtgatcgc
     2341 gggggaaatc ggattcccgc tggagttcac cacattgcct tgcgaatcca tcgaaaaagc
     2401 gccgtcccgc gtgtaggcga tcgttccgtc cggcatggtg acctggaaga agcccagacc
     2461 ggtgatcgac atgtccagat tgccctgggt ctgcagcacg ctgcctgtgt gtagttcttt
     2521 tcggtggccg cgatgcgcac cccggtgccc agggagagcc cggtggagta ctgcgtgttt
     2581 tgcgtgctct gtgcgcccac ctgctgtacg ttctggtaca tcagatcctg gaatgccgct
     2641 cggtcggcct tgaagccggt ggtgttggta ttcgcgagat tgttcgcgat caccgacatc
     2701 tgggtgtttt gtgcgtcgag gccggttttt gcggcccata gtgcgagatt catggtggat
     2761 tcctttgtct cttggctctt gttctcaagc tgccatcatc atgcgctgag cgctttgttc
     2821 gttctggtcc gtggagctga tgctcttgat ctgcaattcg tacagacgct gcagttcgat
     2881 catgttgacc aatgactggg cggcgttgac gttgctcgat tcaagctcgc cggaaacgac
     2941 ggtcaccgtt tcgtccgtct tggcctggcc gccgctcttg aggcgcaaga gaccgtcggg
     3001 tcctttctgc agatccttcg tggagggatt caccagcttg atccggtcga cctgcgattg
     3061 ggccgcggcg gaaaggccga gcggcactac cgacacggtg ccgtcggtcc cgatggtcac
     3121 ctgggtcgag ggaggcaccg tcaagggacc ggactccgac agcacgggga accccgtcga
     3181 ggtgatgacc gcgcccaacg cagtcacctg cagatcgcct gcccgggtgt aggcctcttt
     3241 gccgtcggca ccgcgcaccg cgatgaaccc cttgccgttg accgcgatat cgagcggact
     3301 gccggtgttc atcaggacac cgctcgagaa atccgtgccc gcatcgccgg cgacggcgtt
     3361 gacgcgggtg gcgtaccctg gtccgtagat cggctcgctg gtgaacgcga cgccgtccgc
     3421 acggaagccc gtggtgctgg aattcgcgat gttgtggttg ttgacggcct gcgcttgcat
     3481 gatttgcttc gcgccggtca tcgccacgta cagccctctg tccatattta aagtcctatc
     3541 ggtacgagtc gattcgatct agacgttcgc ggtgttactc gcgccatcaa tggccgatgt
     3601 tgatgatggt ctgcgattct tggttggccg tcgtgatgac ctgcgcattg gcctggaacg
     3661 cccgctgcgc cgtgatcatg tcgaccagct gcgtggtcaa atcgacgttg gaactttcca
     3721 gcgctccgga ctgaatgctg ccgaagcctg cggatcctgc gactcccgaa acgaccgtgc
     3781 ccgaggtgaa ggtttccgac cagttcgtgt cgcccagcga cttcaagccc tgcgggttcg
     3841 ggaaattcgc catcgccaat tggccgagct gcgtggatcg gccgttggtg tagaccgccg
     3901 agacgatgcc ggtcgggtcg atcgccacgg tgctgagctg acccgtcgca tatccgttct
     3961 gggtgatgga ggtgacgccg aattggccgc cgtactgcgt cgtttgtccg aagttgaagg
     4021 ccaatgacat gggctgcgcg ccgctggtcg gggtgaagcc gttgaaattg agcactccgc
     4081 ccactggggc ggtcacctgt cccgagctgc cgaaggtcag tgtttgcgtc ggtccggccg
     4141 agacaccgtt caccgtcata tccaccgacc aggtattggc gggtgcagcc tgctgcgaga
     4201 agaacatggt ggccggatag gcgttgccca gcgagtcgta caccgtggtc gaagtcgact
     4261 ggttgtaggt gagcgagttg gccgggttga acggcgcgac cgagggcggc gtggtattcg
     4321 ccggcaggtt caggatcacc gtaccggtgg tggtggccaa cggcgcgctc tgcgcggttt
     4381 gcaggttgag gttcgacaga gtgccggtgt tgaagccgcc gttgaccagc ggcgggtaca
     4441 cctgcagcgc ctcgcccgtg gaactgatga cgttgccgga cttgtcctcg ccgaactggc
     4501 cgttgcgcga gtagaccagg ccgttggcgc tcttcagcgt gtagaagccg tcgccgctga
     4561 tcgcgagatc gaggttcgag gaggacgtgg tgatgttgcc ctgcgtgaac tgctgccgcg
     4621 tgctcgacag gcgcacgcct tgtccgatga cggagttgtt gaggttcacg gcgccggcgg
     4681 cgtagacgtc ggcgaattgc gcgcgcgcat ccttgaagcc gatggtgttc gcgttggcga
     4741 tgttgttcgc ggtgacgtcc agatccgaac tggcggccgt caatcccgat agtgctgtgc
     4801 tgaatgccat ggaagtagtc ctttagtggt tcaatttgaa atttgcttga cgccgctgaa
     4861 cggcacgctg ccgagtccgc tcacgttcaa ggtaagtccg gtctgtccgg cgcccatcgt
     4921 gacgctctga acgatcccgt tcacatacgt ggtggccgcg gtggcccccg atgcgccgcc
     4981 gttgaactgc gcgctcaagg tgtactggcc ggcgggcgcc tgcgagccgt cctgcagctt
     5041 tccgtcccag gagaaatttg cgagcccgtt cgattgcgcg ccgagattga tctggcgcag
     5101 cagcgcgccg ctgctgtcgg agatgttgac caccgcctgc gaagtggtct gcggcacctc
     5161 gatggcgccg gatacggatc cgttcgccgt gagattggcc gtcttactgc tgaccagtgc
     5221 ctgatgaccg agcagcgaag acgcctgcag cgcctggctc gacaccagcg aactcgacaa
     5281 cgcgccgaat gaggtgttca gctgcgtgat gccctgcacc gtgctcaggg aggcgagctg
     5341 gttcaggaac tggttgctgt cgaccgggct ggtcgggtcc tgattcttga gctgcgcgag
     5401 catcagcgtc aggaaattcg tgccgccgag ggtcgccgcg ccggcggccg gggcgctgcc
     5461 ggagctgccc gaagcggagc tcgtgctcgt cgcgccggtg tagctcgggt tggggaatag
     5521 tacggaagaa gtcatgaata attcctagtg tttatcaatg tcccatcgcg atggtcttga
     5581 gcagcaggtc gcgcgccgtg ttgatgcact cgacgttggt cgcaaaggag cggttggccg
     5641 agatcatgtt ggtcatctct tcgatcgagt tgacgttcga catgaagacg tagccgtcct
     5701 tgttggcgag cggatggtcc ggctgatagc gcatggcgga gggcgccttg ctctccacca
     5761 cgccaaccac ccgcacgccg gccgcggccg cctcgccgtc gaatccggct tggccggcgt
     5821 ccatcatggt ttggaatacc ggatggcgcg cccggtagac cttgctttcg tcgccgttga
     5881 cgctttcggc attggcgaga ttgctggccg tggtattgag ccgtatggtc tcggcattga
     5941 gggcgctgcc ggcgacgttg aacacattga acagagacat cttctttctc cttggcggcg
     6001 tgccggttac cggcccgcgc ctattgaccg gcaatggcaa actgcagttc ggcgattctg
     6061 ctgttgatga acatgaggct cgcctggtag cgcacgttgt tctccgagaa ggctgcctgt
     6121 tcgaccggca tttccaccgt gttgccgtcc agggacgcct ggaacgggtt gcggtatttc
     6181 aattcgccgc cgccctcctc ggcatcgttc agggcgatat gcgaagcttg cgtggtgctc
     6241 atgggcagcc gcgcggacgc gtcggtgccg gcgagcactt cgctgaaatc gatgtcgcgc
     6301 gccttgaagt tcggcgtatc ggcattggcc atgttcgctg cgaggactcc gatgcgttgt
     6361 ccgtgcagca gcagcgcccg ctcatgaatg ccaaaaatgt tgtcgaacat gtcgttgatc
     6421 tcctcgggtg gggataaagc aagtgctgtg ccaagcgctg ataacgggcg attggctgcc
     6481 cggccggctg attcccgcaa catggcagac gccggtggcc gcggcggcgg caaggacttg
     6541 ccgcgggcgg cgagcgggcg gcagccggcg ggccggcggc agttgagcct gggagcctgt
     6601 ctgagtattt tccgtgcggg tggcctgccg ccccgcaccg gcgcatcgca agcgcgatgt
     6661 gctcgtcctt tccgtgggcg atccaggagt ggaagcggcg gtcacggcac gtggcttgcc
     6721 cctcatcagc catcaccggc gcagtcgcaa gcgcgacggt gcccgtcctt gccgtgggcg
     6781 atcccatgaa aggacctggc ggacggtcat ggcacgtggc ttgcacctca taatcccatc
     6841 ttgcgcaaac cgtcaaaatt ccgtcgggcc gccctcctat t
//
LOCUS       DATGEM010000029         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_446508, whole genome shotgun sequence.
ACCESSION   DATGEM010000029 DATGEM010000000
VERSION     DATGEM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446508"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VKQ27_00775"
     CDS             complement(<1..324)
                     /locus_tag="VKQ27_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87491.1"
                     /translation="MRAHQNRQRTASHTREYSEAQIQSADVLVVRAAQPAHKKPGPVI
                     RMSVNRGRRHLPTLPTAFDHDVDCRIAKTAKVVVKLSAGGPASNPGTEQQRQRNETER
                     RDRRVG"
     gene            442..810
                     /locus_tag="VKQ27_00780"
     CDS             442..810
                     /locus_tag="VKQ27_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLY87492.1"
                     /translation="MLWAVGFIFAFTIGGVTRVVLANAGIVQILHNTHYVIAHFHFVL
                     SLGSVCGIFAGFYFWIGKMSGRQYPEFWGWPGCRGDIRIIPRCSPVGIRVVDRRVHRC
                     GRVGCVPVCRAPHVDGRRTG"
     gene            complement(880..2322)
                     /locus_tag="VKQ27_00785"
     CDS             complement(880..2322)
                     /locus_tag="VKQ27_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HLY87493.1"
                     /translation="MARALIGRTIRRLRADRRLTQQALAVKLGISASYLNLIEHDQRA
                     VTASLLIKLGELLGVDLATLSGRTERQLEVALREAFADPLLSADAVPDAEIAELAAAA
                     PNAAQAVLALYRAWRVAREDAGGIALPSGRRVLLPNEEARDFFDDRANHFAPLEIAAE
                     AIAAQLGASPAEMNHAIAERLRRTHGLAVTVQLLEGALRRYDPGARLLVLSETLPRES
                     RGFHMAFQLALLEAREPVETVLAEAAPSSPEAAMLIRVGLLNYVAGALLMPYAGFLQA
                     ATTLRHDVEALASRFGVSFEQACHRLSTLQRPGARGVPFFFVRVDPAGNVSKRFSAAG
                     FPFARYGGSCPRWIVHTAFSQPGQMQVQIAELPDGAAYVCFARTVMRPVRAWGEPPPT
                     HVVAMGCALTHADAVAYADGLDLERAKIGIGLSCRLCDRPDCRSRAFPPLEHRLALDP
                     NSAGLAPYRFETRQRSAGSANVQRTRGSSA"
     gene            2464..>3208
                     /gene="aceA"
                     /locus_tag="VKQ27_00790"
     CDS             2464..>3208
                     /gene="aceA"
                     /locus_tag="VKQ27_00790"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HLY87494.1"
                     /translation="MHLIPDPCRFDGIRRDYTQQDVDRLSGRFRVRHTLAEMGATRLW
                     QLLNTEPFVPTLGALTGNQAVQQVKAGLQAIYLSGWQVAADANTAGTMYPDQSLYPVD
                     SVPNVVRRINNALRRADQIARLEDERGTYWMAPIVADAEAGFGGALNAFELMKAMIEA
                     GAAGVHFEDQLASEKKCGHLGGKVLVPMQQHIRTLNAARLAADVEGVPTVLLCRTDAH
                     SAQLLTSDVDERDWPFISGERTPEGFFRMK"
BASE COUNT          562 a          977 c         1086 g          583 t
ORIGIN      
        1 gcctacacgg cgatcgcggc gttctgtctc gttccgctga cgttgttgtt ccgtcccggg
       61 attgctggcc ggcccgcccg ctgacagttt gacgacaact ttagcggttt tcgcgatgcg
      121 acaatcaaca tcgtggtcga aagccgtggg gagggtcgga agatgtcggc gaccgcgatt
      181 cacgctcatg cggatcacag gcccgggttt tttgtgcgct ggttgtgcag cacgaaccac
      241 gaggacatcg gcactctgta tttgtgcttc gctgtattcg cgggtgtggc tggcagtgcg
      301 ctgtcgattc tgatgcgcgc gcaatgcagc atccggccgg cacgattgtc acatcgggcc
      361 aggagtggaa cacgatcgtc acggcccacg gcccgacgat gatcttcttc accgtaatgc
      421 cggcattccg atcgagcgcc gatgctgtgg gcggtcggct tcatcttcgc attcaccatt
      481 ggcggtgtga ctagagtggt attggcaaat gcgggcatcg tccaaatcct gcacaatacc
      541 cactacgtga ttgcgcactt ccattttgtg ctgtcgctcg gttcggtatg cgggattttc
      601 gccggatttt acttctggat cggcaagatg tcgggcaggc agtacccgga attctggggt
      661 tggccgggat gccgcggcga tatccggatt atcccgaggt gttcgccggt tggaattcgt
      721 gtcgtcgatc ggcgcgtaca tcgctgcggc agggttggtt gtgttcctgt atgtcgtgct
      781 ccgcacgttg atggccggcg aaccggttga cgccaattat tgggggatgg agcgacaacc
      841 ttggagtgga ctgtgtccct cgcccccgcc gttccacacc tatgccgagc tgccgcgggt
      901 gcgctgaacg ttggcgctac cggctgatcg ctggcgggtc tcgaagcggt agggcgcaag
      961 accggccgaa ttcggatcaa gagccagacg atgttcgagc ggcgggaatg cgcggctgcg
     1021 gcaatcgggg cgatcgcaga gccggcagga cagaccgatc ccgatctttg ccctttcgag
     1081 atccagccca tcagcgtacg ccacggcgtc ggcatgggtc agcgcacatc ccatcgccac
     1141 gacatgcgtg ggaggcggct cgccccaggc acgcaccggt cgcatcaccg tccgcgcgaa
     1201 gcagacatac gcggcgccat ccggcagttc ggcgatctgc acctgcatct ggcccggctg
     1261 ggagaaggcg gtgtggacga tccagcgcgg acaggagccg ccatagcgcg cgaatggaaa
     1321 gccggccgcg gagaagcgtt tcgacacgtt cccggcgggg tcgacacgca caaagaagaa
     1381 cggcacgccg cgcgcgccgg gccgctgcag cgttgagagc cggtggcaag cctgttcgaa
     1441 cgacacgccg aagcgcgagg ccagagcctc gacgtcatga cgcaaggtgg tggctgcctg
     1501 caggaatcct gcgtagggca tgagcaacgc ccctgcgacg tagttgagca gaccaacgcg
     1561 gatgagcatg gctgcctcag gcgaagacgg cgcagcctcg gccagcacgg tctccactgg
     1621 ctctctcgcc tccagcaggg cgagctggaa cgccatgtgg aagccgcgac tctcgcgcgg
     1681 cagtgtttcg gaaagcacca gcagccgcgc gcccggatcg taacggcgca atgcaccctc
     1741 caacagctga accgtgacag ccagcccatg ggtccgccgc aggcgttcgg cgatcgcatg
     1801 gttcatctcg gcgggcgatg cgccgagttg cgccgcgatc gcctcggctg cgatttccag
     1861 aggcgcaaaa tgattggccc tgtcgtcgaa gaagtcgcgg gcctcttcat tcggcaacag
     1921 cacgcggcgc cctgacggaa gcgcgatgcc gcccgcatcc tcgcgcgcca cccgccaggc
     1981 gcgatatagc gcgagcacgg cctgcgctgc attcggcgca gctgcagcga gttcggcgat
     2041 ctcggcgtcg ggcaccgcgt cggcggagag cagcggatcc gcgaaggcct cgcgcagcgc
     2101 gacctcgagc tggcgctcgg tacggccgga caacgtggcg aggtctacgc cgagcagctc
     2161 ccccagcttg atcagcagcg atgcggtcac tgcgcgctga tcgtgctcga tcaggttcag
     2221 atagctggcc gagatgccga gcttcaccgc aagcgcctgc tgggtcagcc ggcgatcggc
     2281 tcgcaggcgg cggatggtgc ggccaatgag cgcacgcgcc actgatttac atcctttaca
     2341 aacttgcggc cttcactgtg aaggctgtga cagcatttcc gggcagtttg gcagatttca
     2401 ggtgcacttc gcatctgcaa tgtcaattat tgacgcgttg attgtgcaaa caggagagcg
     2461 caggtgcatc tgatccccga tccctgccgg ttcgatggaa tccggcgcga ctacacccag
     2521 caggacgtcg atcgcctgtc cggacggttc cgagtccgcc acacgctggc ggaaatgggc
     2581 gcgacccggc tgtggcagct gctgaacacc gagccgttcg tgccgacgct gggggcactg
     2641 accggcaacc aggcggtgca gcaggtgaag gcggggctgc aggcgatcta cctgtcaggc
     2701 tggcaggtcg cagcggacgc caataccgcc ggcacgatgt acccggacca gtcgctgtat
     2761 ccggttgatt cggtgcccaa cgtggtgcgg cggatcaaca acgcgctgcg ccgtgccgat
     2821 cagatcgccc ggctggaaga tgagcgcggc acgtattgga tggcgccgat cgtggcggat
     2881 gccgaggcgg gatttggcgg cgcgctgaat gcgttcgagt tgatgaaggc gatgatcgag
     2941 gccggtgcgg cgggcgtgca tttcgaggac cagctcgcgt cggagaagaa gtgcggccat
     3001 ctcggcggca aggtgctggt gcctatgcag cagcatatac gcacgctgaa tgccgcgcgg
     3061 cttgcggcgg atgtcgaggg cgtgccgacc gtgctgctct gccgcaccga cgcacattcg
     3121 gcacagttgc tgaccagcga tgtcgacgag cgggattggc cgttcatttc cggtgaacgc
     3181 acgccggagg gcttcttccg gatgaaac
//
LOCUS       DATGEM010000030         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1136296, whole genome shotgun sequence.
ACCESSION   DATGEM010000030 DATGEM010000000
VERSION     DATGEM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136296"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VKQ27_00795"
     CDS             complement(<1..514)
                     /locus_tag="VKQ27_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HLY87495.1"
                     /translation="MAAIILSREALMPYPTHLLPTTVVGSYPQPDWLVNRSALEKHGV
                     PRTHAQDIWRIPPDLLEQAQDDGTILAIRDMERAGIDIITDGEIRRESYSNRFALALE
                     GIDAEHPGEVRSQQGRVTPVPRVVGKIRRRSAVEVRDVQFLLRQTDRATKITLPGPFT
                     LSQQAKNEFYK"
     gene            557..1633
                     /locus_tag="VKQ27_00800"
     CDS             557..1633
                     /locus_tag="VKQ27_00800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87496.1"
                     /translation="MEFGIFHEFPSLPGRSHADAFAQSFELVDGAERWGLDAMWLGEL
                     HFDPGRSVLSAPLCIASAIAMRTQRMKIGLAVQVLPLCHPLRLAEEAATVDQLSGGRL
                     IFGVGRSGVARTYEAYGVPYGESRERFAEILDIVRKAWTQEKVSYQGKFHHFEDVAVV
                     PKPFQQPVPEIRVAATSADTFVENGRRGLPIFVAVRYETAAEIAPHIADYRTALAAAG
                     HAGGGVFLRVPAYIAPTDAQARAEAEPSLIHFFRQQAALLADSAKRAGVDGADRRAQT
                     ARRLEKMTYEEALQGTILIGSPDSVARRLEELRQELGLDGVLMELNCGGRIPHDNVMS
                     ALRLLCQEVMPHFAAPDGGKRTIN"
     gene            1689..4550
                     /locus_tag="VKQ27_00805"
     CDS             1689..4550
                     /locus_tag="VKQ27_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY87497.1"
                     /translation="MSRTAPSPEGLRASGGQPAKDVVSFGPFQLSPSARLLERDRKPV
                     QIGGRALDILIALVERAGEVVSKADLFAKVWADVVVDEGSLRFHVSALRKALGDGKSG
                     ARYIVNAPGRGYSFVAELERSEMGAATPAPAASPSLPTQLTRMVGRADTVSRLSDELA
                     RYRFVTVVGPGGIGKTTVAVTVAHGQIAAFDGAICFMDFGALRDPRLVAGALASTLGL
                     MVSSEDPVPGLLAFLRERRMLLILDSCEHVIESLAPLAESICKAAPQVHLLATSRESL
                     RVEGEHIHRLFPLEFPPPAAGVTATETLVYPAVQLFVERVAASAGGYELTDVDAPVVA
                     EICQRLDGIALALELAAGRVAAYGIQGTARLLNSRLSLLWQGRRTAVPRHQTLSAALG
                     WSYDLLSPAEATVLRRLSVFAGPFTLEATIAVGVSGDVAQSDVIEALASLAAKSLVAV
                     DHVKGAVRYRLLDATRAYVQDKLAESGETGEIALRHAQYFADFLTRVGRDAPDDFPVC
                     AEHLSNVRAALEWCFSAAGDPTLATALAAVATQLLLEVSLLTECYRWAERAMSALDAS
                     VIGTRQELQLQAAVAVSAMFTQGSTDQVRNAFVRGIELTRLLSDPHFELRLLRGFHIY
                     LTRIGDYRRALELSERAVIVARTVDDRADRLTADWMLGVAQHLKGDQAEAVVHCGSAL
                     SAALPAHQRNVLRLGYDDRIIALVALARALWLAGSPDRAVEAARFTISEAERLDQPVT
                     LGIALIWMTYVFLWCGDWVGAEQLIERLIGHAAKHSLGPYNAVGLGLKGDLLVRRGEP
                     AEGVDLLRRGLATLHATRHKILVGAFSIALAEGLARIGQIDEALIVVEEAIQQVVHEN
                     ETFDMPEVLRLKGVLLHARPRSDVAVAEAWLRQSLDLAREQSALSWELRTATSLARVL
                     ADSGRADEARTLLRPVYERFAEGHATADIRDAKAFLASH"
BASE COUNT          701 a         1385 c         1688 g          865 t
ORIGIN      
        1 ccttgtaaaa ctcgttcttc gcctgctgcg acagggtgaa cggcccgggc agtgttatct
       61 tggtcgcgcg atcggtctgg cgcagcagga actggacatc gcgcacctcc accgcggagc
      121 gccggcggat cttccctacc acgcggggca ccggtgtcac ccggccctgc tgcgagcgca
      181 cctcacccgg atgctcggcg tcgatgccct caagtgccag cgcgaaccgg ttcgaatagc
      241 tttcgcggcg gatctcgcca tcggtgatga tgtcgattcc ggcgcgctcc atgtcgcgga
      301 tcgcgaggat cgtgccgtca tcctgcgcct gttccaacag gtctggcgga atgcgccaga
      361 tgtcctgtgc gtgcgtccgc ggcacgccgt gcttctccag cgccgagcgg ttcaccagcc
      421 aatcgggctg cggatagctg ccgacaacgg tggtgggcag caggtgcgtc gggtagggca
      481 tgagggcctc gcgtgacagg atgatggcgg ccattctagg ctttccgcca aggttttgaa
      541 cgggagggcg tctggcatgg aattcggtat cttccacgag ttcccctcgt tacccgggcg
      601 atcccacgcg gacgccttcg cgcagtcgtt cgaactggtg gatggtgccg aacgctgggg
      661 gctcgatgcg atgtggctcg gcgagctgca tttcgatccg ggccggtcgg tgctgtcggc
      721 gccgctgtgt attgcgagcg ccatcgcgat gcgtacacag cggatgaaga tcggtctggc
      781 ggtgcaggtg ctgccattgt gccatccgct gcgtctcgcc gaggaagccg cgacggtgga
      841 ccagctcagc ggcgggcggc tgatattcgg cgttggccgc agcggggttg ctcgcaccta
      901 tgaggcgtat ggcgtgcctt atggcgaaag ccgcgagcgg ttcgcggaga tcctcgacat
      961 cgtgcgcaag gcgtggacgc aggagaaggt ctcgtatcag ggcaagttcc atcacttcga
     1021 ggacgtggcg gtggtgccga agccgttcca gcagccggtt ccggagatcc gtgtcgcggc
     1081 aaccagtgcc gatacgtttg tagagaacgg ccggcgcggg ctgccgatct ttgtcgcggt
     1141 acgctacgag acggcggcgg agatcgcgcc gcatatcgcg gactatcgca cagccctggc
     1201 cgcagcgggg catgcgggtg gcggcgtgtt cctgcgcgtg ccggcctata tcgcgcccac
     1261 tgacgcgcag gcccgcgcgg aagcggagcc gagcctgatc cacttctttc gtcagcaggc
     1321 agcactgctt gccgattccg ccaagcgcgc gggagtcgat ggtgcggacc gacgcgcgca
     1381 aacggcacgg cggctggaaa agatgaccta tgaggaggcg ctgcaaggca ccatcctgat
     1441 cggctcgccg gactcggtgg cgaggcgatt ggaggagctg cggcaggaac tcggactgga
     1501 cggcgtgttg atggagctga actgcggcgg ccggattcct catgacaacg tgatgagcgc
     1561 gctgcgcctg ctgtgccagg aggtgatgcc gcactttgcc gctccagacg ggggcaagcg
     1621 caccatcaac tgattggtcc accgcatccc catgcccaag tggcgcgggg tccggtatgc
     1681 tgcggatcat gagtcgcacg gccccatcgc cggaaggttt gcgcgcgtcc ggtggccagc
     1741 cggcgaagga cgtcgtttcg ttcggtccgt tccagttgtc gccatcagcg cgattgctgg
     1801 aaagggaccg aaagcccgtc cagatcgggg ggcgggcgct ggatatcctg atcgccctgg
     1861 tcgagcgcgc aggcgaggtt gtcagcaaag cggatctgtt cgccaaggtc tgggcggatg
     1921 tcgttgtcga tgaaggcagc ctgcggttcc atgtttcggc gctgcgcaag gcgctggggg
     1981 atggaaaatc gggcgcgcga tacatcgtca acgcacccgg tcgcggctac tcgttcgtgg
     2041 cggagctgga gcgatccgaa atgggggccg caacgccggc tcctgcagcc tcgccgagcc
     2101 tgccaacgca gctcaccaga atggtgggcc gcgcggacac tgtctcgcgc ctgtcggatg
     2161 agctcgcgcg gtatcgtttt gtgaccgttg tcggcccggg cggcatcggc aagaccacgg
     2221 tcgcggtgac ggtcgcgcat gggcagatcg ccgcgttcga cggcgcgatc tgtttcatgg
     2281 atttcggcgc gctgcgggat ccgcggctgg tggcgggagc gctcgcctcg accctcggac
     2341 tgatggtgag ttccgaagat ccggtgcccg gcctgctcgc gttcctgcgg gagcggcgga
     2401 tgctgctgat cctcgacagc tgcgagcacg tcatcgaaag cctggcgccg ctggctgaaa
     2461 gtatctgcaa ggcggcgccg caggtgcatc tgctggcgac cagccgggaa tcgctgcgcg
     2521 ttgagggcga acacatccat cgcctgttcc cgctggaatt cccaccgccg gccgccggcg
     2581 tcacggcgac ggagaccctc gtgtatcccg ccgtgcagct tttcgtggag cgcgtggcgg
     2641 catcggccgg cggttatgag ctgaccgatg tcgatgcgcc ggttgttgcg gaaatctgcc
     2701 agcggctcga cggaattgct cttgcactgg agttggcggc tggccgggtc gctgcttatg
     2761 ggatccaggg caccgcacgg ctgctcaaca gccggctctc gctgttgtgg cagggccggc
     2821 gaacggcggt gccgcggcat cagacgctca gtgccgccct tggttggagc tacgacctgc
     2881 tgtcgccggc cgaggccacg gtgctgcgac ggctttccgt cttcgccggt ccgttcacgc
     2941 tggaagccac cattgcggtc ggtgtgtcgg gagacgtggc ccagtcggat gtgatcgagg
     3001 cactcgccag cctcgccgcg aagtcgctgg tagcggtgga tcatgtgaag ggggcggtgc
     3061 ggtatcggct gctcgatgcg acccgcgcct acgtgcagga caagctggcc gaaagcggcg
     3121 agaccggcga gatcgcgctg cggcacgcac aatatttcgc tgacttcctc acccgggtcg
     3181 gacgcgatgc gcccgacgat ttcccggtct gcgcggaaca tctgtccaac gtgcgagcgg
     3241 cgctggagtg gtgcttttcc gccgcgggtg atcccacgct tgcgactgcc ctggcggcgg
     3301 tcgccacaca gcttttgctc gaagtctcgc tgctgacgga gtgctatcgc tgggcggaac
     3361 gcgcgatgtc cgcgctcgat gcatctgtca ttggcacgcg ccaggaattg cagttgcagg
     3421 cagcggttgc ggtatcggcg atgttcactc agggcagcac cgatcaagtg cggaatgcgt
     3481 tcgtccgcgg catagagctc acccggttgc tcagcgatcc gcatttcgag cttcggctgc
     3541 tgcgcgggtt ccacatctac cttacgcgga tcggcgatta tcgccgtgcg ctggaattga
     3601 gcgaacgcgc cgtgatcgtc gccaggacag tggatgatcg tgccgatcgc ctgacggcgg
     3661 actggatgct gggggtcgcg cagcatctga agggcgatca ggcggaggcg gtcgtgcatt
     3721 gcggcagcgc gctcagtgcg gcgttgccgg cgcatcagcg caatgtcctg cgcctgggct
     3781 acgacgacag gatcatcgct cttgttgcac tggcgcgcgc gctttggctc gcgggctcgc
     3841 cggatcgtgc ggtggaggca gcgcggttca ccatcagcga agcggagcgg ctggatcagc
     3901 ctgtcacgct ggggatcgcg ctgatctgga tgacttatgt ctttctctgg tgcggcgact
     3961 gggtgggggc cgagcagctt atcgagcggc tgatcggcca tgcggcgaag cattcgcttg
     4021 gtccctacaa tgccgttggc cttggcttga agggagatct tctggtcagg cggggtgagc
     4081 ccgcggaagg cgtcgatctg ctgcgccgag gcctcgccac gctgcacgcg acgcggcaca
     4141 agatcctggt gggtgcgttc tcgatagctt tggcggaggg gctggcgcgg atcggccaga
     4201 tcgatgaggc gctgatcgtg gtggaggagg cgatccagca ggttgtgcac gaaaacgaaa
     4261 cgttcgatat gccggaggtg ctgcggctga agggtgtgtt gctgcatgcc cggccgcggt
     4321 cggatgtcgc ggtggccgag gcctggctcc gccagtcgct cgatcttgcg cgcgagcaat
     4381 cggcattgag ctgggagctg cgcacggcga cgagcctggc acgcgtgctt gcggacagcg
     4441 gtcgcgcgga tgaggcgcgc actctgctgc ggccggtgta tgagcggttc gcggaagggc
     4501 acgcgactgc cgatattcgg gatgcgaagg ctttcctggc gtcacattag tgggtcaggg
     4561 cggggcggtg gcgatggcgg cggccgggtc gagcgggttg accgggggct cgccgatgaa
     4621 gaagccgagg acctggcgg
//
LOCUS       DATGEM010000031         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_445438, whole genome shotgun sequence.
ACCESSION   DATGEM010000031 DATGEM010000000
VERSION     DATGEM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445438"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VKQ27_00810"
     CDS             <1..1221
                     /locus_tag="VKQ27_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLY87498.1"
                     /translation="KEGGKIMNALYKGVIVSGVLAAVAFYFITNRMMGDSPLGAMHLF
                     YASLVGLVLTALMVVITEYYTATEYAPVRHVAAASQTGHATNIIAGLGVSMKSTALPV
                     LAVCAAIWAAYSFAGLYGIAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMAGLPAK
                     IRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHNLEALHKLGPDTLVKGFE
                     LSDPAVIIGLFIGGLVPYLFGAMAMEAVGRAAGAVVNEVRRQFREIKGIMEGTAKPDY
                     SRAVDMLTKAAIKEMLIPSLLPVLVPVVVAFGMNWLMGPGAGIRALGGLLIGTIVTGL
                     FVAISMTTGGGAWDNAKKYIEDGHYGGKGSDAHKAAVTGDTVGDPYKDTAGPALNPLI
                     KIINIVALLLVPLL"
     gene            complement(1297..1692)
                     /locus_tag="VKQ27_00815"
     CDS             complement(1297..1692)
                     /locus_tag="VKQ27_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87499.1"
                     /translation="MNPALRRSIGILLVAGLYGGAYADDKSTAAADADLARLRNDITT
                     LIGAAKCSNLVNCRIAALGVNACGGPTEYIAYSWLSTDKADLETRIAEYNFALEDAQK
                     LGVPVPACVALPEPVAACINGRCVIPAGR"
     gene            1874..>2292
                     /locus_tag="VKQ27_00820"
     CDS             1874..>2292
                     /locus_tag="VKQ27_00820"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HLY87500.1"
                     /translation="MERGYYRDHTLTVARHPSETDERMMVRLLAFALNADETLVFGAG
                     LSTDDEPDLWRKDLTGSIETWIDVGLPDEKRIRRACGRANAVFVYSYGGHAAELWWQQ
                     VGDKLARSHKLTVIDFPQAGTRALAGLAARNMQLQVT"
BASE COUNT          435 a          753 c          690 g          414 t
ORIGIN      
        1 aaggaaggcg gcaagatcat gaacgcgctc tacaagggcg tgatcgtgtc gggcgtgctc
       61 gccgccgtcg ccttctactt catcaccaac aggatgatgg gtgacagccc gctgggcgcg
      121 atgcatctgt tttacgcctc gctcgtcggc ctggtcctca ccgcgttgat ggtggtcatt
      181 accgagtact acaccgccac cgaatatgcg ccggtgcgcc acgtcgccgc tgcctcgcag
      241 acgggccacg cgaccaacat catcgccggt ctgggcgtct ccatgaaatc caccgcactt
      301 cccgttctcg ccgtttgcgc cgcgatctgg gcggcatact cgtttgccgg actttacggc
      361 atcgccatcg ccgccaccgc catgctgtcg atgaccggca tgatcgtcgc actggatgcc
      421 tacggcccca tcaccgacaa cgccggcggc attgcggaaa tggcaggctt gcctgcgaag
      481 atccgcgaca tcaccgaccc gctcgacgcg gtgggcaaca ccaccaaggc ggtgaccaag
      541 ggctatgcca tcggctcagc cggccttgcc gcgctggtgc tgttcgccga ctacacccat
      601 aacctggaag cgctgcacaa gctcggaccc gacacgctgg tcaagggctt cgaactgtcc
      661 gacccggcgg tcatcatcgg cctgttcatc ggcggactgg tgccttacct gttcggcgcc
      721 atggcgatgg aagccgtggg acgcgctgcc ggtgcggtgg tgaacgaagt ccgccgccag
      781 ttccgcgaga tcaagggcat catggaaggc acggccaagc ccgactactc gcgcgcggtg
      841 gacatgctga ccaaagccgc catcaaggaa atgctgattc cgtcgctgct gccggtgctg
      901 gtaccggtgg tggtcgcctt cggcatgaac tggctgatgg gtccgggtgc gggcattcgc
      961 gccctcggcg gcctgctgat cggcaccatc gttaccggcc tgttcgtcgc catctcgatg
     1021 accactggcg gcggcgcctg ggacaacgcc aagaaatata tcgaagacgg ccactacggc
     1081 ggcaagggct ccgacgcgca caaggcagcg gtgaccggcg acacggtagg cgatccctac
     1141 aaggatacag ccggcccggc gttgaacccg ttgatcaaga tcatcaacat cgtcgcgctg
     1201 ctgctggtgc cgttgctgta agcacaacag cgagtcaaaa gaaagtcccg cagcgcgcaa
     1261 gccctgcggg atttttttcg gccgcggtat tgccgctcaa cggccggcag gaatcacgca
     1321 gcggccgttg atgcatgcag ccaccggttc cggcagtgca acgcatgcgg ggacgggaac
     1381 tcccagcttc tgcgcatcct ccagcgcgaa gttgtattcg gcgattctgg tttcgagatc
     1441 ggccttgtcc gtcgacagcc acgaatacgc gatgtattcg gtcgggccgc cgcaggcgtt
     1501 cacgccgagc gcagcaatcc ggcaattcac caggttcgag cacttcgccg ctccgatcag
     1561 cgtggtgatg tcgttgcgca atcgtgcaag gtccgcgtcc gctgccgccg tggatttatc
     1621 atccgcgtac gccccgccgt acagaccggc cacaagcagt atgccaatcg accttcgaag
     1681 tgccggattc atgcatacct ccccgacttg tcggctgcca ctatttcacg tgcgggacag
     1741 ggggcgcaag ccgccggatt cgactcggtc accgctttct gagacagtac cgacccttca
     1801 gactatagca ccctacgaca atccaatggc ccttaaggca accatcttca aagcggagtt
     1861 gcacatcgcc gacatggagc gcggctacta ccgcgaccac acactcaccg tcgcgcgtca
     1921 tccatcggaa actgacgagc gcatgatggt gcggttgctg gcctttgcgc tgaacgccga
     1981 tgagaccctg gtgttcggcg ccggactgag cacggacgac gaaccggacc tgtggcgcaa
     2041 ggacctgacc ggcagcatcg aaacctggat cgacgtcggc ctgccggacg agaaacgcat
     2101 ccgcagggcc tgcggccgcg ccaatgcggt gttcgtttac agctacggcg gccatgccgc
     2161 agaactctgg tggcagcagg tcggcgacaa actcgcgcga tcgcacaagc tcacggtcat
     2221 cgattttccg caagcaggaa cgcgagctct tgccgggctt gctgcccgca atatgcaatt
     2281 gcaggtcacc at
//
LOCUS       DATGEM010000032         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_900431, whole genome shotgun sequence.
ACCESSION   DATGEM010000032 DATGEM010000000
VERSION     DATGEM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900431"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..3310)
                     /locus_tag="VKQ27_00825"
     CDS             complement(128..3310)
                     /locus_tag="VKQ27_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY87501.1"
                     /translation="MGIVRFALKYPHTFYVLAGLMLFLGISAIVVAPKDIFPAIDIPV
                     VTVIWQETGLTPDQMEQRVTTYSEYSISSNVSDIRNMESQTLSGLTVQKIYFQPNVNI
                     DLAISQIASATNAIRALMPAGIQPPIIVQYSASSVPVLQLSLSSDTLNEQQLYDYGIY
                     QIRQALAPIPGITLPTPYGGKYRQIMVDLDPDALRARGITPADVLTAVNAQNLTLPSG
                     DAKLGERQMIVRVNAMPLTIAALNRVPIKQVGQTTVYLSDVAHVRDGWAVQQNIVHAD
                     GKRSVLLTIIKNGDASTLDVVNRVKAALPAIRKAAPPGMRINLLFDQSVFVTDAITSV
                     VREGVIAAGLTALMILVFLGSWRSTLVVMVSIPLSILTSLAVLTALGQTINTMTLGGL
                     ALAVGILVDDSTVTIENTHRLLEDGTEFDTAVLNGAAGIAVPTLISTLAICCVFVSVF
                     FLQGAARYLFTPLAMAVVFAMLASYGISRTLTPIVIRLLLRSEHENHGAAPGWLGRFH
                     VRFNAGFDRFKDFYDWLLAGIMRRRILTPAVACLVVAGATVLSLNVGRDFFPQVDAGL
                     IQLHVRAPARTRIERTEQIFASVEQAIRQQIPPKDLGLVLDNIGLPQRVYNMAFTDGT
                     TIGVNDGQILLQLNEGHAPTADYIRQLRRVLPVQFPDLQFYFQPADLATQVLNFGVPS
                     QIDVQVQGRDRAANIAIAGNLRQQLANIPGLVDVHLQQELNAPELSYTIDRTRAQQLG
                     LSLQQVANDLNISLSSSEQVSPNFWTDPKNGIPYYFAVQTPEYRLATKNDLDNTPIGG
                     SLSDGAVVPDTFGNVASAQRELVESVYNHSNIQPVYDVYASLQSRDLGSAAAEIQRVV
                     HSLQGQLGPGDRIVIRGQIESMNSAFAQLTIGLLFAAVFVYALMVVNYQSFLDPLAVI
                     LALPGAGSGIVLMLYVTGTTLSVPSMMGAIMSVGVASANSILLVTFAREQRQIGMSAF
                     DAAMSAGTTRLRPVLMTAAAMMVGMVPMAIGGPGEEQNAVLARAVIGGVVVGTMTTLL
                     FVPYLYSVVGQFERRRAELPRSLMQHGAST"
     gene            complement(3703..>4438)
                     /locus_tag="VKQ27_00830"
     CDS             complement(3703..>4438)
                     /locus_tag="VKQ27_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLY87502.1"
                     /translation="AGAIREGLVGAFVDKSDAYDPVLWTTRHELLGSFLSMGLALAIR
                     RTSVKASGGITVCVAFLAGAADPWLVPFVAGTGLALLVCNRRMTLGWLSTAASIGVGL
                     FLFGYLEPAGAYSGMAVIQDGAGYRYDRILVHTLAAVLMMFGIIANEGAGRVLNARPL
                     LFLGRLSFPIYLFHFPLLCSLGCGLFLALREVTSYGGSTIIVAVIYAAAVLMVGVLLT
                     RVDEAWGAWLSRMTDSLFMPETRISK"
BASE COUNT          884 a         1374 c         1422 g          758 t
ORIGIN      
        1 agcgccgcaa taccaagccc gagccagcgc gcggtccgcc ggcgatggcg ttcgcgctgc
       61 ggatcggctg ctgtcgcctg ctcgctgtcg cgctgcaagg catgtggcgg cgtgggagat
      121 tgttcgttca tgtcgaggcc ccgtgctgca tcaggcttcg cggcagttcg gcacgccgcc
      181 gctcaaattg tccgacgacc gaatagaggt agggaacgaa cagcagcgtg gtcatggtgc
      241 cgacgaccac gccgccgatg accgcgcgcg cgagcacggc attctgctcc tcgcccgggc
      301 cgccgatggc catcggcacc atgccgacca tcatcgccgc ggccgtcatc agcactggac
      361 gcagacgcgt ggtgccggcc gacatcgcag cgtcgaaggc gctcatcccg atttggcgtt
      421 gttcgcgcgc gaaggtcacc agcaggatcg agttcgccga ggccacaccg accgacatga
      481 tggcacccat catcgacggc acgctcagcg tggtgcctgt cacgtacagc atcaagacga
      541 tgccactgcc cgcaccaggc agcgccagga tgacagcgag cggatcgagg aagctctggt
      601 agttcaccac catcagcgca tagacgaaca ccgccgcgaa cagcaggccg atggtcagtt
      661 gggcgaaggc gctgttcatg ctttcgatct ggccgcggat gacgatgcgg tcaccggggc
      721 cgagctggcc ttgcaggctg tgaacgacac gctggatctc cgccgcggca ctcccaagat
      781 ctcgactctg caggctggca tacacgtcgt agactggctg gatgttggag tgattgtaga
      841 cggactcgac taattcgcgc tgtgcggacg ccacgttgcc gaacgtgtcg ggcaccaccg
      901 cgccatcgga cagcgaaccg ccgatcggcg tgttgtcgag atcgttcttg gtcgcgagcc
      961 ggtattccgg cgtctgcacc gcgaagtagt acgggatgcc gttctttgga tcggtccaga
     1021 agtttggtga aacctgctcg gacgaactta ggctgatgtt caggtcgttg gcgacctgct
     1081 gcaggctcag gccgagctgc tgagcccgcg tgcgatcgat cgtgtaggac agctccggcg
     1141 cattgagttc ctgctggaga tgcacatcca ccaggcccgg aatgttcgcg agctgctgac
     1201 gcagattgcc ggcgatggcg atattggcgg cgcgatcgcg cccttgcacc tgcacatcga
     1261 tctgcgacgg cacgccgaag ttgagcacct gtgtcgcgag atcagccggc tggaaataga
     1321 actgcaagtc aggaaactgc accggcagga cccggcgcag ctgcctgatg tagtccgcgg
     1381 tcggtgcgtg accttcgttg agctgcaaca ggatctggcc gtcattcacg ccgatcgtgg
     1441 tgccatcggt gaacgccatg ttgtatacac gctgcggcag cccgatgttg tccagcacca
     1501 ggccaaggtc cttgggtggg atctgctgac ggatggcttg ctccaccgag gcgaagatct
     1561 gctcggtgcg ttcgatgcgg gtgcgcgccg gggcacggac gtgaagctgg atcaggccag
     1621 cgtcgacctg agggaagaag tcgcgtccga cattcagtga cagaactgtc gcaccggcga
     1681 cgacaaggca cgcgacggca ggcgtcagga tgcgccgccg catgatgccc gcgagcagcc
     1741 agtcgtagaa atccttgaac cggtcgaacc cggcattgaa ccgaacgtgg aagcggccaa
     1801 gccagccggg cgcagcaccg tggttttcgt gctcgctgcg cagcagcaga cgaatgacga
     1861 tgggtgtgag cgtgcgcgat atgccgtagg aggcaagcat cgcgaacacc acggccatcg
     1921 ccagtggcgt gaacaggtag cgcgccgcac cctgcaggaa gaataccgag acaaacacac
     1981 agcagattgc cagcgtggag atcagcgtcg gcaccgcgat gcccgcggca ccgttcaata
     2041 ccgcggtgtc gaactcggtg ccgtcctcga gcagccggtg ggtgttctcg atcgtgacgg
     2101 tcgagtcatc gaccaggatg ccgacagcga gcgccaggcc gccgagtgtc atcgtgttga
     2161 ttgtctgtcc gagcgctgtc agcaccgcga gcgaggtcag gatcgacagc gggatcgaca
     2221 ccatgaccac cagcgtcgaa cgccacgaac caaggaacac caggatcatc agcgcggtca
     2281 gcccggcggc gatgacaccc tcgcgcacaa cgctggtgat ggcatctgtc acaaagaccg
     2341 actgatcgaa cagcagattg atccgcatgc cgggcggcgc ggctttccgg atcgcaggga
     2401 gtgctgcctt cacgcgattc accacatcca gcgtggaggc gtcgccgttc ttgatgatgg
     2461 taagcagcac cgagcgcttg ccgtcggcgt ggacgatgtt ctgctgcacc gcccagccat
     2521 cgcgcacatg cgccacatcc gacagataaa ccgtggtctg tcccacctgc ttgatcggca
     2581 cgcgattcag cgcggcaatc gtcagcggca tggcgttgac ccgcacgatc atctggcgct
     2641 cgcccagctt ggcgtcgccg gaaggcagcg tcaggttctg cgcgttcacc gcggtaagca
     2701 catcggcggg cgtgatgcct cgtgcccgca acgcgtcggg gtcgagatcg accatgatct
     2761 gccgatattt cccgccatat ggcgtgggca gggtgatccc ggggatcggc gccagcgcct
     2821 ggcggatctg gtagatgccg taatcataca gctgctgttc gttcagcgta tcggagctga
     2881 ggctgagctg cagcaccggc accgacgatg cactgtattg cacgatgatc ggcggctgga
     2941 tgcccgccgg catcagcgcc cggatggcgt tcgtggcaga ggcgatctgg ctgatcgcga
     3001 gatcgatgtt gacgttgggc tggaagtaga tcttctgcac cgtcagaccc gacagcgtct
     3061 ggctttccat gttgcggata tcgctgacgt tcgagctgat cgagtactcg ctgtaagtgg
     3121 tgacacgctg ctccatctga tcaggcgtaa ggccggtctc ctgccagatg acggtgacga
     3181 cggggatgtc gatcgccggg aaaatatcct tcggcgcaac cacgatggcg ctgatgccga
     3241 ggaacagcat caggcccgcc aacacataga aggtgtgagg atatttcagg gcaaatcgga
     3301 cgatacccaa gatgagcgct ccagaggcaa ggtcgtgccg atatcagcga ccggcttcgc
     3361 gaacgttttt tgacaatgcc cctcggacgc tgcgtgatct ccccgcagcg gccggggatc
     3421 gcgcggctcg gtatgaagtg gtgccgatgg ttcaaggcgc aagcttgggc aggcgccgaa
     3481 tgcgcacccg cgtgcccata ggtttcatgc gcggagcggt gaaatggcgc gatgcggccc
     3541 ggcgctatcg ccccgtcatc gtggccacgg caccggaaaa ggcacgcgcc accgccccga
     3601 gatgatgcaa cgcgggctgg ctagttcgaa gcgtacttca cttcgacaaa ggcatcgacg
     3661 cgcccagcgc agcagcgccg ctgaccgcaa gcgctgtccg tttcatttgg atatccgtgt
     3721 ctccggcatg aacagggaat ctgtcatgcg gctgagccag gcaccccacg cttcatctac
     3781 tcgcgtgagc aatacgccaa ccatgaggac cgccgcagcg tagatcaccg caacgatgat
     3841 cgtgctgccg ccataggacg tgacttcgcg cagcgcgagg aacaggccgc agccgagcga
     3901 gcaaagcaac ggaaaatgga acaggtaaat ggggaacgat aacctgccaa ggaagagcag
     3961 cggtcttgcg ttcagcacgc ggcctgcgcc ctcattcgca atgatcccga acatcatgag
     4021 caccgcagcc aaagtatgca ccaggatccg gtcgtatcgg tagcccgcac cgtcttggat
     4081 gaccgccatg cctgagtagg ctcctgctgg ctcgaggtaa ccgaacagga atagccccac
     4141 accaatgctg gcggcggtcg acagccaccc aagggtcatt ctccggttgc agacaagcag
     4201 agccagcccc gtcccagcga caaatgggac cagccaagga tccgcagccc ccgccaggaa
     4261 cgcgacacac actgtgatgc cgccggatgc cttcacggat gtgcggcgga tagccagcgc
     4321 caggcccatg ctgaggaaac tgccaagcag ttcgtgacgc gttgtccaga gcaccggatc
     4381 ataggcatcg gacttgtcga cgaacgcccc gaccaggccc tcacggatcg ctcctgcc
//
LOCUS       DATGEM010000033         9474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_796459, whole genome shotgun sequence.
ACCESSION   DATGEM010000033 DATGEM010000000
VERSION     DATGEM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796459"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VKQ27_00835"
     CDS             complement(<1..761)
                     /locus_tag="VKQ27_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY87503.1"
                     /translation="MERASVCTLDCPDTCSLSVTVEDGRVTKVRGSDALPYTEGVICN
                     KVAREMGAFVHGPQRILHPLRHTGPKGAGAFERITWTEALDTIYERFTAVIDRWGPQA
                     VAPLNYAGPHGMLAGDSMSSRFFHKLGASQLYRRSMCGGVRSEAWSGTYGAVPGCPPD
                     FAANANLNVVWGNNATVTNLHLVRNVRRAMRKGGRLVVVDPLRTKIAEQADLHLAPLP
                     GTDVLLAWALATELERMGAFDTAFIERHVLGFEEF"
     gene            872..1720
                     /locus_tag="VKQ27_00840"
     CDS             872..1720
                     /locus_tag="VKQ27_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLY87504.1"
                     /translation="MTNWTETYRGAVPPWQCDVTEHFTIAYYFDRLEEAEASLADDLG
                     LNELPRRTGLIRRIDARFARELREGASFHVESAALDADGGLRLGHRFVDSANGEVVTW
                     FDEHLELPAPLAPQRRATIETRLAVWDGPTTEKRPEVQVTDSFIPTARGRVKPGDVNA
                     AGNFALGAIVHRFSNASGQLGAAIGADAAYLQQQRRGFSTFELILRTSGELPLDAPYL
                     VRTGIAHLGNSSLRMVHCMTNAQTGAEVARLSQFGVNLDLDARRPATWPEDLRHRAAA
                     LVVPAG"
     gene            complement(1724..2614)
                     /locus_tag="VKQ27_00845"
     CDS             complement(1724..2614)
                     /locus_tag="VKQ27_00845"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87505.1"
                     /translation="MAITVRQLHPLFCAEIGGVDTGKPMDDATFADIRAALDEYSVLV
                     FHDQSLDDERQIAFSRRFGPLEMAGKANPAVGTYFARQSNLDIATGDVIPVEDRRMIY
                     QKGNYLWHSDSSFKPVPSLCSLLSARVVPSGGGNTEFATMRAAYAALPSVLARQLAAL
                     VAEHSLVYSRGTVSTGSLTAEMKAELPGAWQSLIRVNPINGRKAVYAGAHASHVIGWP
                     REQGRAFVQWLTEFATQPQFRYSHAWRQGDLVIWDNRAVLHRATAYDTLRQKRLMQRT
                     TVRDSGPTAAQPEQYELAAE"
     gene            2735..3493
                     /locus_tag="VKQ27_00850"
     CDS             2735..3493
                     /locus_tag="VKQ27_00850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87506.1"
                     /translation="MATSEKCAVITGAGSGIGRATALALHADGFSVVLAGRRRDMLEQ
                     TAALAKATPSGMLVVPTDVTDPSSIAALFDTVKATYGRIDLLFNNAGTSTRNIPIEDL
                     TYEQWSTVVATNLTGAFLCAQHAFRMMKSQTPRGGRIINNGSVSAHVPRRNSTPYVAT
                     KHGMTGLTRSLSLDGREFDIACGQIDIGNAATTRNEDTARGRLQATGRVEAEARIDVN
                     DVARGVAYMASLSLEANVQFMTVMATKMPYIGRG"
     gene            3564..4730
                     /locus_tag="VKQ27_00855"
     CDS             3564..4730
                     /locus_tag="VKQ27_00855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87507.1"
                     /translation="MDHSLPTDPHAEIRVEVAKLCARFPGEYWRKLDRERAYPTEFVQ
                     ALTEAGYLAALIPEEYGGAGLPLSAAAAILETIHAEGCNGAACHAQMYIMGTILRHGS
                     PKQKQEYLPKIATGELRLQAFGVTEPTSGTDTTSLRTFARRDGDHYVVNGQKVWTSRA
                     EHSDLMLLLARTTQRDQVKRRTDGLSTFIVDMRKSVGHGMTIRPLRTMMNHNSTEIFF
                     DNLMVPADNLIGEEGKGFRYILDGMNAERLLISAECIGDARWFLKKATDYARDRQVFG
                     RPIGQNQGIQFPLARAYAQMRAAELMIREGCAKFEAGLTPGEEANMAKLLGADASWAA
                     AEACLQTHGGFGFAEEYDVERKYRETRLYQVAPISTNLILSYIAEHVLGLPRSY"
     gene            complement(4748..5443)
                     /locus_tag="VKQ27_00860"
     CDS             complement(4748..5443)
                     /locus_tag="VKQ27_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLY87508.1"
                     /translation="MQRFWVFALMLIAVAGPVRAEPVAATGAPQGLLCRAAVAAAEHA
                     NGIPAHLLAAISRVESGRPDPETGNLHPWPWTANAEGQGYFYDTKAQAVAAVRDMQTH
                     GVQSIDIGCGQVNLMHHPNAFPSLDVALDPQANAAYAGKFLKELFDQTGDWTKAVAMY
                     HSATPNIGDDYRKKVLAAWPEEVRMPNAVSRSPLARAWGATIAAPAGRSLGAHRAMPV
                     RLAMQASPTRIGK"
     gene            complement(5680..6555)
                     /locus_tag="VKQ27_00865"
     CDS             complement(5680..6555)
                     /locus_tag="VKQ27_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HLY87509.1"
                     /translation="MDIPFTTTAIASTDPVVVRRPLRQTAPIVFASPHSGRCYPDELL
                     AASRLDPLSLRRSEDSFVDELFIAAPEYGAPLIAATFPRAWCDANREAWELDQAMFAD
                     RLPGWVNTTSARVGAGLGTIARVVASGEAIYRDKLLFIDAEQRIRSCWQPFHDTLEGL
                     IAETQATFGGCLLIDCHSMPTQGHVSRSGVKPADFVLGDAHGTACSPLVTRLVEGVLA
                     AQGFSVRRNDPYAGGYITRHYGRPREGIQALQIEIARDLYMDEARIERLTGFADVQAA
                     VTRLIASLTERLPLP"
     gene            6643..6717
                     /locus_tag="VKQ27_00870"
     tRNA            6643..6717
                     /locus_tag="VKQ27_00870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6675..6677,aa:Val,seq:gac)
     gene            complement(6737..6943)
                     /locus_tag="VKQ27_00875"
     CDS             complement(6737..6943)
                     /locus_tag="VKQ27_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87510.1"
                     /translation="MIDILERHSMMRVISTAPSRRLDASELVLSDRLITLAEDADRAG
                     YIDTAEMLLNLACSVFDEAPRKPH"
     gene            complement(6965..7285)
                     /locus_tag="VKQ27_00880"
     CDS             complement(6965..7285)
                     /locus_tag="VKQ27_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HLY87511.1"
                     /translation="MTVGYQSTGSGSRENWVESDDRVSAAGRRSLAGLIKRSFSLAGH
                     RTSVALEEEFWAALGHIAAERQQTITALVAATDAGRSAGQPLASALRVLALREFFPVP
                     LTSS"
     gene            complement(7343..8398)
                     /gene="queG"
                     /locus_tag="VKQ27_00885"
     CDS             complement(7343..8398)
                     /gene="queG"
                     /locus_tag="VKQ27_00885"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLY87512.1"
                     /translation="MGAADPKPLIRERALALGFDAVGFCRAELGPLARERLRDFLASG
                     QHGDMGWLAARSEARGDPTQLWPEARSVIALGLTYAPEDDPLASLTLRDRGTISVYAR
                     NRDYHAVVKGMLKHLAQFVVSRFGPEVKVFVDTAPVMEKPLAMRAGLGWQGKHTNLVS
                     RAYGSWLFLGEIYTTLAIAPDEPHSDRCGTCSRCLSVCPTDAFPAPYRLDATRCISYL
                     TIEHNGPIPHELRPLIGNRIYGCDDCLAACPWNRFARGTTHDKLRAREDMTAPRLAEL
                     ALLDDAGFRALFSGSPIKRIGRDRFVRNVLIAIGNSGEGSLLPIAERLQHDPHPVVAE
                     AAAWAFQRLSAAMVTLP"
     gene            complement(8385..8849)
                     /gene="queF"
                     /locus_tag="VKQ27_00890"
     CDS             complement(8385..8849)
                     /gene="queF"
                     /locus_tag="VKQ27_00890"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HLY87513.1"
                     /translation="MTDPAYAGLTQLGHHVEQPASPDVAKLERVANPSRGKHYVVRFT
                     CPEFTSLCPITGQPDFAHIVIDYIPCDWLVESKSLKLYLTAFRNHGAFHEACTMAIAD
                     KLIALLDPVWLRIGGYWYPRGGIPIDVFWQTGAPPDGAWIPPQDVAPYRGRG"
     gene            complement(8846..>9474)
                     /locus_tag="VKQ27_00895"
     CDS             complement(8846..>9474)
                     /locus_tag="VKQ27_00895"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLY87514.1"
                     /translation="MRWAERSRDAFRQRDGYGLFGIVQGSIYPDLRAASARALTTIGF
                     DGYAVGGLAIGEGQATTFTVLDATVPHLPTDAPRYLMGVGTPDDLIGAVQRGIDMFDC
                     VIPTRAGRTARAYTSRGVFNLRNARFADDAAPLDTACFCLACTRHSRAYLHHLFKAEE
                     ILGPMLLTWHNVHYYQDLMRGLRAAIASRGLEAFASATRAGWAPEELS"
BASE COUNT         1736 a         3124 c         3017 g         1597 t
ORIGIN      
        1 atgaactcct cgaagccaag cacgtgccgt tcgatgaacg cggtatcgaa cgcccccatc
       61 cgttccagct cggtggcaag cgcccacgcc agcagcacat ccgtgccagg caaaggtgcc
      121 agatgcagat ccgcctgctc ggcgatcttg gtgcgcagcg gatccaccac caccagccgg
      181 ccgcccttgc gcatcgcgcg gcgcacattg cgcacgaggt gcaggttggt gacagtcgcg
      241 ttgttccccc acacgacatt gaggttcgca ttggcggcaa agtccggcgg gcagccgggg
      301 acagcgccat acgtgccgct ccatgcctcg ctacgcacgc cgccgcacat ggaccggcgg
      361 tagagttgcg acgcgcccag cttgtggaag aagcgcgagg acatgctgtc gcctgccagc
      421 atgccgtgtg gcccagcgta gttgagcggc gccacagcct ggggacccca gcgatcgatc
      481 acggccgtga agcgctcgta aattgtgtcg agcgcctcgg tccaggttat gcgttcgaag
      541 gcgccggcgc ccttcgggcc ggtgtggcgc agcggatgca ggatgcgctg cggaccatgc
      601 acgaaggcgc ccatctcgcg cgccaccttg ttgcagatga caccctcggt atacggcagc
      661 gcgtcagagc cgcgcacctt ggtgacgcgg ccgtcctcga cagtgacgct gaggctgcac
      721 gtgtccgggc agtcaagtgt gcatacgctg gcgcgttcca tggcaagact cctctggctc
      781 gaccgtatcg caaggacgtg cggccgtcag tgccgattac cgctggcgat gctagaccaa
      841 gcgcccgaca cagcaggacc gaattcgaac gatgaccaac tggaccgaaa cctatcgcgg
      901 cgcggtaccg ccctggcaat gcgacgtcac cgagcatttc accatcgcct actactttga
      961 tcggctggag gaggcggaag ccagtctcgc cgacgacctg ggcctgaatg agctgccgag
     1021 gcgcaccggc ctcatccgcc ggatcgacgc cagattcgca cgcgaactgc gcgaaggcgc
     1081 gagctttcac gtcgagagtg cggcactcga tgcggatggc ggcctgcgcc tcggccatcg
     1141 cttcgtcgat tccgccaacg gcgaggtggt gacctggttc gacgagcatt tggagttgcc
     1201 cgcgcccttg gccccgcagc ggcgggccac gatcgagacc cgcctcgccg tctgggatgg
     1261 cccgactacc gagaagcgtc ccgaagtcca ggtgacggac agtttcatcc ccaccgcacg
     1321 cgggcgggtc aaaccgggcg atgtcaacgc ggccggcaat ttcgcacttg gcgccatcgt
     1381 gcatcgcttc tccaacgcat ccggccagct tggtgccgcg atcggcgcgg atgccgcata
     1441 cctgcaacaa cagcggcgcg gcttctcgac cttcgagctg atccttcgca catcgggcga
     1501 actgccactc gacgcgcctt acctggtgcg caccggaatc gcgcatctcg gcaactcgtc
     1561 gctgcgcatg gtgcattgca tgaccaacgc acaaaccggt gctgaggtag cccggctcag
     1621 ccagttcggt gtgaacctcg acctcgatgc gcggcgcccg gccacttggc cggaggacct
     1681 ccgccaccgt gccgctgcgc tcgtggtacc ggcaggctga acgtcattcg gcggccagct
     1741 cgtattgctc gggctgagct gccgtcggcc cggagtcgcg caccgtcgtg cgttgcatca
     1801 gccgcttctg ccgcagcgtg tcgtaggcgg tcgcgcggtg cagcacggcg cgattgtccc
     1861 agatcaccaa gtcgccctgc cgccacgcat gagagtagcg gaactgaggc tgcgtcgcga
     1921 actcggtcag ccactggacg aatgcgcggc cctgttcgcg cggccagcca atcacgtgcg
     1981 aggcatgtgc gccggcatag accgccttgc gcccgttgat cggattgacg cgtattaggc
     2041 tctgccaggc gccaggcagc tcggctttca tctctgccgt cagcgaaccg gtggataccg
     2101 tgccccgcga atagacgagg ctgtgttccg caaccagagc cgcgagttgc cgtgccagca
     2161 ccgacggcag cgcggcatac gccgcgcgca tcgtggcgaa ttcggtgttg ccgcccccag
     2221 acggcacgac acgcgccgac agcagcgagc acaatgacgg caccggcttg aacgaactgt
     2281 ccgagtgcca gaggtagttg cccttctgat agatcattcg ccgatcctcg accggaatca
     2341 catcgcccgt ggcaatgtcc aggttcgatt gacgcgcgaa ataggtgccc actgcgggat
     2401 tggccttgcc ggccatctcc agcggaccaa agcgccggct gaatgcgatc tggcgttcgt
     2461 catccaggct ctgatcgtgg aataccagga cggagtactc gtccagcgcc gcacggatgt
     2521 ccgcgaaggt cgcatcatcc atcggcttgc cggtatccac gccaccgatc tccgcacaga
     2581 acagcgggtg cagttgccga accgtgatcg ccatcgccgc aggtcctcat gcgatttgcc
     2641 gcgagcgtac tgcctacggt ccgcgctgcc cagcggttgc cctggttcga cccggcggtg
     2701 accatggagg cgaagcgaga ggagacatcc agccatggca acgtcggaaa agtgcgcggt
     2761 gatcaccggt gcaggcagcg gcatcggacg ggccacggcg ctggcgctgc atgcggacgg
     2821 gttttcggtg gtgctggcgg gacgccgtcg ggacatgctg gaacagaccg ccgcgctcgc
     2881 caaggcgaca ccgtccggga tgcttgtggt gccgacggat gtgaccgatc ccagctcgat
     2941 cgcggcgctg ttcgacacag taaaggcgac ctacggccgc atcgacctgc tgttcaacaa
     3001 cgcgggtacc agcacccgca acattccgat cgaagacctg acctacgaac aatggtcgac
     3061 cgttgtcgcc acgaacctga ccggtgcgtt cctgtgcgcg cagcatgcat tccgcatgat
     3121 gaagagccag acgccacgtg gcgggcgcat catcaacaac ggatcggtct ccgcccatgt
     3181 gccgcgcagg aactctacgc cctatgtcgc gacgaagcat gggatgaccg gcctgacccg
     3241 atcgctatcg ctcgacggcc gcgaattcga catcgcctgc ggccagatcg acatcggcaa
     3301 tgccgcgacg acacgcaacg aggacacggc acgcggccgg ctgcaggcca ccggccgggt
     3361 ggaagcggaa gcgcggatcg acgtgaacga tgtcgctcgc ggcgtcgcct acatggccag
     3421 cctgtcgctt gaggccaacg tgcagttcat gaccgtgatg gcgacgaaga tgccgtatat
     3481 tggccgcggc tgaggttcac gccaccttct tgcaccccct cccccatccg cctaacacga
     3541 aatgccgctg cgggagttgc gccatggacc attcgcttcc aaccgaccct cacgctgaga
     3601 tccgcgtcga ggtcgccaag ctctgcgccc gcttccccgg cgaatactgg cgcaagctgg
     3661 atcgtgagcg cgcgtatccc accgagttcg tgcaggcgct gaccgaggcc ggctacctcg
     3721 ccgcgctgat ccccgaggag tatggcggag ccggcctgcc gctgtccgcg gcggctgcca
     3781 tcctcgaaac gatccacgca gaaggctgca acggtgctgc ctgtcatgcg cagatgtaca
     3841 tcatgggcac catcctccgg catggctcac ccaagcagaa gcaggagtat ctgccgaaga
     3901 tcgccacggg tgagctccgc cttcaggcgt ttggtgtcac cgagccgacc agcggcaccg
     3961 acaccacctc gctgcgcacc ttcgccaggc gcgacggcga ccactacgtg gtcaacggcc
     4021 agaaagtctg gaccagtcgc gccgagcatt ccgacctgat gctgttgctg gcgcgaacca
     4081 cgcagcgcga ccaggtgaag cggcgtactg atggcctctc caccttcatc gtcgatatgc
     4141 gcaagtcggt cgggcacggc atgaccatcc ggccgctgcg caccatgatg aaccacaact
     4201 ccaccgagat cttcttcgac aatctgatgg tgcctgctga caacctgatc ggcgaggaag
     4261 gcaaaggatt ccgctacatc ctcgacggca tgaatgctga gcggctgctg atctcggccg
     4321 agtgcatcgg cgacgcccga tggttcctga agaaggccac ggactatgcc cgagatcgcc
     4381 aggtgttcgg ccggccgatc ggccagaacc aggggatcca atttccgctt gcccgtgcct
     4441 acgcgcagat gcgcgccgca gagctgatga tccgcgaggg ctgcgccaaa ttcgaggccg
     4501 ggctcacacc gggcgaggaa gccaacatgg ccaagctgct cggtgctgat gcctcatggg
     4561 ccgccgcgga ggcttgcctg cagacccatg gcggcttcgg ctttgccgag gaatacgacg
     4621 tggagcgcaa ataccgcgag acccggctgt atcaggtggc acccatcagc accaacctga
     4681 tcctttccta tatcgcggaa cacgtgctgg gcctgccccg gagctattga gctgagtcag
     4741 ggggtgctca cttcccgatc cgggttggcg atgcctgcat ggccagcctg accggcatcg
     4801 cccggtgggc accaaggctg cggccagccg gcgctgcaat cgttgctccc caggcccgcg
     4861 ccagcggaga tctgctgaca gcgttcggca tccgtacctc ctcgggccat gcggccagga
     4921 ccttcttgcg gtagtcgtcg ccgatattgg gcgtcgctga atggtacatc gcgaccgcct
     4981 tggtccagtc accggtctgg tcgaacagct ccttcaggaa cttcccggca taggcggcat
     5041 ttgcctgtgg atcgagcgcc acatcgagac ttgggaaggc gttcggatgg tgcatcaggt
     5101 tcacctggcc gcagccgatg tcgatcgact gcacaccatg cgtttgcatg tcacgcaccg
     5161 ccgcgacggc ctgggccttc gtgtcgtaga aatagccctg cccttcggca ttcgctgtcc
     5221 acggccatgg atgcagattg ccggtctcag ggtcgggtcg cccgctctcc acccggctga
     5281 ttgcggcgag caggtgagcc gggatgccgt ttgcgtgttc agccgccgcc accgcggcac
     5341 ggcagagcag cccctgtggc gcgccggtgg ccgccacagg ctcggcacgg accggccctg
     5401 ccacggcaat cagcatcaga gcgaagaccc agaaccgctg catgccgcca tcctgcgccc
     5461 gagggtttag tgaactgttg ccgccggcga ccctgggaag ccaaaaattt tgggcaatgc
     5521 tgcattgcaa aaaatcgctt gcatgcgaat gaccgatccc ctagattgtg catcgcagca
     5581 atgcggcccc tggccgcgct gtctgctttc ttggacgttt cctccctaaa cttggcggcc
     5641 cgcaagggtc gccaattttt ttgtccgggc gagcggacct cagggaagcg gcaaccgctc
     5701 ggtcagactc gctatcagcc gcgtcaccgc agcctgaacg tcagcgaacc ccgtgagccg
     5761 ttcaatccgc gcctcatcca tatagagatc gcgggctatc tcgatctgca gcgcctgtat
     5821 gccttcacgc ggccggccat aatgccgcgt gatgtagcca cctgcgtagg gatcgttccg
     5881 acgcaccgag aatccctgtg cggccagcac cccctccacg aggcgggtaa ccaagggcga
     5941 acacgcggtt ccatgcgcgt cgcccaacac gaaatcggct ggcttaactc ctgagcggct
     6001 gacgtgtcct tgcgtgggca tcgagtggca gtcgatgagc aggcaccctc cgaatgtcgc
     6061 ctgcgtctcg gcaatcagcc cttccaacgt gtcatggaac ggctgccaac agctgcggat
     6121 gcgctgttcg gcatcgataa acagcagctt gtcccggtag atcgcctccc ctgaggcaac
     6181 gacccgggcg atcgtcccga gcccggcacc gacacgcgca cttgtcgtgt tgacccagcc
     6241 gggcagtcgg tcggcgaaca tggcctggtc cagctcccac gcctctcggt tcgcgtcgca
     6301 ccaggcccgc gggaaggtcg cagcaatcaa cggcgcacca tattctggcg cggcaatgaa
     6361 gagctcatca acgaaactgt cctcgctgcg ccgcaggctc agcgggtcga ggcgcgaggc
     6421 tgccaatagc tcatccgggt agcaccgtcc cgagtgcggc gaggcaaaca cgattggcgc
     6481 cgtctgtcgc agcggacgcc gcaccaccac cggatcagtc gaagcaatcg cggtggtggt
     6541 gaatggtatg tccatggccc tatagaagcc gccgccgcgc gcggtgtcac gaccctaacc
     6601 ttgcccgccg cgctcagcac gcgtattttt tccccggcgg atgggcgcgt agctcagcgg
     6661 gagagcatcg ccttgacatg gcgggggtca caggttcaat ccctgtcgcg cccaccatcc
     6721 gctctgcacg gcacgatcag tgcggcttgc gcggtgcctc gtcgaacaca ctgcaggcca
     6781 ggttcaacag catttcagct gtatcaatat agccggcacg atccgcgtct tcggccaggg
     6841 tgatcagccg gtcggacagc accagttcgc tggcatccag acggcgcgat ggcgccgtac
     6901 ttataacgcg catcattgaa tgcctctcta aaatgtcgat cacagcattt ttgcaacctg
     6961 tggtttagga cgaggttaac ggcactggga aaaattcacg cagcgccaac acccttaatg
     7021 cagaggccag tggctgaccg gcgctgcggc cagcgtcagt ggcagcgacc agggcagtga
     7081 tcgtctgctg gcgctccgcg gcgatatggc ccagcgccgc ccagaactcc tcctcgaggg
     7141 cgacactggt gcgatggcca gcgagcgaaa agcttctctt gatcagccca gccagagacc
     7201 gcctcccggc agcgctgact ctgtcgtcac tttcaaccca gttctcccga gaacccgaac
     7261 cagtcgattg gtatccgacc gtcatacctg atcggcccgc gcgaagaatg tcacgagctt
     7321 gcgcggtcgc gctggagttt tgtcacggta acgtgaccat agcggcggaa agccgctgaa
     7381 atgcccaggc cgcggcctca gccacgaccg ggtgcggatc gtgttgcaac cgctctgcta
     7441 tgggaagcag cgagccctcg cctgaattgc cgatggcgat cagcacattg cgcacgaaac
     7501 ggtcgcgccc gatccgcttg atgggcgagc cactgaacaa cgcgcggaat ccagcatcgt
     7561 ccagcaaggc aagctcggcc agacgcggtg cggtcatgtc ctcgcgcgcg cgcagcttgt
     7621 catgcgtcgt gccacgggcg aagcggttcc atgggcaagc cgccaggcag tcatcacagc
     7681 cgtagatacg attgccaatc agcggccgca gctcatgcgg gatcggtccg ttatgctcga
     7741 tcgtcagata ggaaatgcag cgggtcgcat cgagccggta gggcgccggg aaggcatcgg
     7801 tcgggcagac gctgaggcag cgcgagcagg taccgcagcg atcgctgtgc ggttcgtcag
     7861 gagcgatcgc cagggttgtg tagatctcgc ccaggaacag ccatgagccg tacgcgcgcg
     7921 aaaccaggtt ggtgtgcttg ccttgccatc ccaggccagc acgcattgcc agaggtttct
     7981 ccatcaccgg cgcggtgtcg acgaacacct tcacctctgg cccgaagcgc gagaccacga
     8041 actgggccag gtgcttcagc atgcctttca ccacggcatg gtagtcgcga ttgcgcgcat
     8101 agaccgagat cgttccgcgg tcgcggagcg tcagagatgc cagcggatca tcctccggcg
     8161 cgtacgtcag gccgagcgca atgacgctgc gcgcctctgg ccacaattgc gttgggtcgc
     8221 cacgcgcctc tgagcgggcg gcaagccagc ccatgtcgcc atgctgccca gaggcgagga
     8281 aatcgcgcag ccgttctcgc gccaggggac cgagctcggc acggcagaag ccaaccgcat
     8341 cgaagccgag cgcgagggcg cgctcgcgga tcagcggctt cgggtcagcc gcgcccacga
     8401 tacggcgcca cgtcctgcgg cgggatccag gcgccatccg gcggagcgcc ggtctgccag
     8461 aacacgtcga tcgggatgcc gccgcgcgga taccagtaac cgccgatgcg aagccacacc
     8521 ggatcgagga gcgcgatcag cttgtcggca atcgccatgg tgcaggcctc atggaaggcg
     8581 ccgtgattgc ggaatgcggt gaggtagagc ttcagcgact tgctttcgac cagccagtcg
     8641 caggggatgt agtcgatgac gatgtgcgcg aagtccggct ggccagtgat cgggcagagc
     8701 gaggtgaatt ccgggcaggt gaagcgcacc acgtagtgct ttccccgtga agggttggcc
     8761 acacgctcaa gcttcgccac gtcggggctg gcgggctgtt cgacgtgatg tcccagttgc
     8821 gtcaggccgg cataggcagg atcggtcatg acaattcctc gggcgcccag ccggcgcgcg
     8881 tggcgctggc gaaagcctcc agtccgcgac tggcaatggc ggcgcggaga ccgcgcatca
     8941 ggtcctgata gtagtgcaca ttatgccagg tcagtagcat cgggccgagt atctcctcgg
     9001 ccttgaacag atgatgcaga taggcacggc tgtggcgcgt gcaagccaga cagaagcagg
     9061 ccgtatcgag cggcgccgcg tcgtcggcga agcgggcatt gcgcaggttg aacacgccgc
     9121 gcgacgtata ggcacgcgcg gtgcggcctg cgcgggtggg tatgacgcag tcgaacatgt
     9181 cgatgccacg ctgcactgca ccgataaggt catccggcgt gcctacgccc atgagatagc
     9241 gcggagcatc cgttgggagg tgcgggacgg tcgcgtccag caccgtgaag gtggtggcct
     9301 ggccctcgcc gatcgcgagg ccgccaacgg cgtagccgtc gaaaccgatg gtcgtgagcg
     9361 ctcgcgcgga ggcggcccgg aggtccgggt agatgctgcc ttggacgatg ccgaacagac
     9421 cgtagccatc gcgctgccgg aatgcgtcgc gcgaccgttc agcccagcgc atgg
//
LOCUS       DATGEM010000034         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_444822, whole genome shotgun sequence.
ACCESSION   DATGEM010000034 DATGEM010000000
VERSION     DATGEM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444822"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VKQ27_00900"
     CDS             <1..777
                     /locus_tag="VKQ27_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY87515.1"
                     /translation="LLPEALENAGRGGADVFVRIDKAVAYADINAAVMPGLRGVVLPD
                     PESAGDVEDVDAMLGEREREAGIPQGELEILVLLGTARGIWNVREIVHASARVASVAI
                     DEARLCQSMRIVPTPELDPLSFSRGRVIVEALAAVRLPLGIGHPLGALPREVGEHELS
                     GLADRARNTGFKGALCPYPSWVAACNTAFTPSDEQVAYYREVRLAFAKGVARGTAAVP
                     FPGGRMIDVPVDERAKLVIDWWERCQRRDAEKAAVARRLL"
     gene            886..>2313
                     /locus_tag="VKQ27_00905"
     CDS             886..>2313
                     /locus_tag="VKQ27_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY87516.1"
                     /translation="MEQQRLLRSGLSRRELLTALGITGASFVAAACTPAATPAPAATS
                     APPAPAATAAKPAAPAATTAPAAPAATAAKPAAPAATTAPAAAKPSITGKITYLDQDD
                     DPASVAWHDVYHKDFMTANPGVTIEDTHYASADYQQKLTTSMATGAQLDFLFWDTTNI
                     PQLWADGRLLPLNDLLENIYTDIGGKDKMSAEALALFTGPNGEIQGIPYYEEPLVWWF
                     RQDLLQAAGLTPPADHWDWQFLLNAVKATHKPPDMYGIGFPTARKTGTLFPIIPFIMN
                     NGGHFVSPDLKDVMFDSQEVRDAYELMKELAQYLPPGTGDWANPQQVDAIAHGTVATG
                     HYYGRVFQNVSLQNKPLIGKLNNTLIPYNKAQPVNWGNWGAHCVLKTAANPDGAKAMI
                     RFSFKKDENIGYLVSVPGLYLPSVPGLAADPAFLNDPTLKLFDSKMVATMTRGAANTA
                     NMTQEGPTWKINQKGGTIYSSLVLAD"
BASE COUNT          451 a          722 c          768 g          372 t
ORIGIN      
        1 ctcctgccgg aggctctcga aaacgcggga cgaggtgggg cggacgtgtt cgtgcgcatc
       61 gacaaggcgg ttgcgtacgc ggacatcaac gccgcggtga tgcccggctt gcggggtgtc
      121 gtcctgcccg atcccgagtc ggcgggcgat gtcgaagacg tcgacgccat gctcggggag
      181 cgcgagcgcg aggcggggat accccagggt gagcttgaaa ttctggtatt gctcggcact
      241 gcgcgaggta tctggaacgt acgcgagatc gtgcatgcca gcgcgcgtgt ggccagtgtg
      301 gccatcgacg aagcacgttt atgccagtcg atgcgcatcg tcccaacccc cgaactcgat
      361 cccctgagtt tttcgcgcgg acgcgtcata gttgaggcgt tagccgcggt caggctgccg
      421 ctgggcatcg gccatccgtt gggtgcgctg ccgcgggagg tgggcgagca cgagctcagc
      481 gggctggcgg accgcgcgcg aaatacgggt tttaaaggcg cgctatgtcc atacccatcc
      541 tgggtggccg cctgcaacac ggcgttcacc ccgagcgacg agcaggtggc gtactaccgc
      601 gaggtcaggc tggcgtttgc caaaggcgtg gcgcgcggaa cggcggcggt gcctttcccg
      661 ggcggacgca tgatcgacgt gcccgtagac gaaagggcaa aactggtgat tgattggtgg
      721 gaacgctgtc agcgccgcga cgcggagaaa gccgcggttg cgcggaggtt gttatgatag
      781 ccgcacgacg attcgtggga gtcacggcgg catcagggcc gggcggtgcg atcttgcgcg
      841 atgggaggga catagcggat gtcggagacg ggtgatgtcc ggagtatgga gcagcagcgt
      901 ctgctgagaa gcggcttgag tcgccgcgaa ttgttgaccg cgctggggat caccggggcg
      961 agttttgtcg ctgcggcgtg caccccggca gcgacccctg cgccagccgc aacgagcgca
     1021 ccgccggcgc cggcagcgac ggcggccaaa ccggccgcgc cagcggcaac aactgcaccg
     1081 gccgcgccgg cggcgacggc cgccaagccg gccgcgccgg cagcaactac tgcaccggcc
     1141 gcggccaaac cgtccattac gggcaagatc acctacctgg accaggatga tgatccggcc
     1201 tcagtggcct ggcacgacgt gtaccacaag gatttcatga cggcgaatcc tggggtcacg
     1261 atcgaggaca cccactacgc ctcggcggac taccaacaaa aactgaccac gtccatggcc
     1321 acgggcgcgc agttggattt cctcttctgg gataccacca acatccccca attgtgggca
     1381 gacggcagac tcttgccgct caacgacctg ctggagaaca tctacacaga catcggcggc
     1441 aaagacaaga tgtcggccga agctctggcg ctgttcaccg ggcccaacgg tgagatccag
     1501 gggattccgt actacgagga gccgctggtc tggtggttcc gccaggatct cctccaggcc
     1561 gccggcctga ccccacccgc cgaccactgg gactggcagt tcctgctcaa cgcggtcaaa
     1621 gccacccaca agccgccgga tatgtatggc attggcttcc ccaccgcgcg caagacgggc
     1681 acgctgtttc cgatcatccc cttcatcatg aacaacggcg gccactttgt gtcgcccgat
     1741 ctgaaggacg tgatgttcga ctctcaggaa gtgcgcgatg catacgagct gatgaaggag
     1801 ctggcgcagt acctgccacc gggcaccggc gactgggcga atccgcagca ggtcgacgcg
     1861 attgcgcacg gcacggtcgc caccgggcac tactacggcc gcgtgttcca gaatgtgagc
     1921 ctgcagaata agccgctgat cggcaaactg aacaacaccc tcatcccgta caacaaggcc
     1981 caacccgtca actggggcaa ctggggcgca cactgcgtgc tcaagacggc ggcgaacccg
     2041 gacggggcca aggcgatgat ccggttcagc ttcaagaaag acgagaacat cggctacctg
     2101 gtgtcagtac cggggctgta tctgccgtca gtgccgggtc tcgccgccga tccggcgttt
     2161 ctgaacgatc cgaccttgaa gttgttcgac tccaagatgg tcgccacgat gacgcggggc
     2221 gccgcgaaca ccgccaacat gacccaggaa gggccaacgt ggaagatcaa ccagaagggt
     2281 ggcacgatct acagctcgtt ggtccttgcc gac
//
LOCUS       DATGEM010000035         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_670678, whole genome shotgun sequence.
ACCESSION   DATGEM010000035 DATGEM010000000
VERSION     DATGEM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670678"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VKQ27_00910"
     CDS             <1..364
                     /locus_tag="VKQ27_00910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLY87517.1"
                     /translation="VIVTTDEDTPVPSVHTVWPVATWWEREAWDLFGIVFSDQPDLRR
                     ILTDYGFEGHPLRKDFPLTGYVEVRYDEDRKAMVYEPVHLTQDFRTFDFMSPWEALTT
                     LPGDEKVHTERAEEGSKP"
     gene            361..1569
                     /locus_tag="VKQ27_00915"
     CDS             361..1569
                     /locus_tag="VKQ27_00915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HLY87518.1"
                     /translation="MSDVEARPTATKVVEIDSHAINFGPQHPAAHGVLRLILEMDGEV
                     VERADPHIGLLHRGTEKLIEYKTYMQAVPYFDRLDYVSPMCEEHAFALATEKLLGIEA
                     PERAQWIRVLFAEITRVLNHLLNVTTYALDVGAITPSLWGFEEREKLMEFHEAASGAR
                     LHANYFRPGGVSKDLPAGLEQRIAAWAETFPKFVDDLEELLTGNRIWKQRTVDIGVMD
                     AKQALAWGFTGPCLRASGVPWDLRRSQPYDKYAEVDFQIPVGRHGDCYDRYLIRLAEM
                     RESVKIVKQALAKMRPGPIKVQDRKFTPPHRAEMKRSMEALIHHFKLYTEGYHVPAGA
                     TYTVVESPKGEFGVYLVADGTNKPYRCKIRSTGFAFLQATEVMARRHMLADTVAIIGS
                     LDIVFGEIDR"
     gene            1566..2189
                     /locus_tag="VKQ27_00920"
     CDS             1566..2189
                     /locus_tag="VKQ27_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HLY87519.1"
                     /translation="MSDFVQPEHFAFDAESEASIPPLLAKYPAGRQASAVIPMLYLVQ
                     NQMKRQTGSAWVPTAGMNEVARRLDMPPIRVYEVATFYFMFNLKPVGKWHLQVCTTTP
                     CWLRGSDEVTATCRAVTGIKDWGETSADGLFTMTEVECVGACVNAPVVQVNDDLYEDL
                     DAETTKQLLDALRRDEPPKVGSMKGRHTSAPEGGPNTLTTLQFGAES"
     gene            2194..>3441
                     /gene="nuoF"
                     /locus_tag="VKQ27_00925"
     CDS             2194..>3441
                     /gene="nuoF"
                     /locus_tag="VKQ27_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HLY87520.1"
                     /translation="MLEDKDRIFTNLYGLHDPYLKGARARGDWDGTKAILEKGRDVIV
                     EEVKASGLRGRGGAGFPTGLKWSFMPKQSDRPCYLIVNADESEPGTCKDRDIMRHDPH
                     KLVEGCLVAGFAMGACAAYIYIRGEFANEAKVLQAAIDEAYEAGLIGKDACGSGYSFD
                     VYLHRGAGAYICGEETGLLESLEGKKGMPRLKPPFPAAVGLYGCPSTVNNVESIAVAP
                     TIMRRGAAWFNSLGRPKNSGPKVFCISGHVNKPCNVEEELGIPLRELIDKYAGGVRGG
                     WDNLLAVIPGGASVPVLPKSICDTVLMDFDSLREARSGLGTAAVIVMDKSTDIIKAIA
                     RLSKFYMHESCGQCTPCREGTGWMWRVMTRMVEGRAEVPEIDTLEQVTRQVEGHTICA
                     LGDAAAWPIQGLIRHFRPEMEARI"
BASE COUNT          643 a          910 c         1264 g          624 t
ORIGIN      
        1 cgtgattgtc actacggatg aagatacgcc ggtgccatct gtgcatacgg tgtggccggt
       61 cgccacgtgg tgggagcgtg aggcgtggga cctgttcggc atcgtgttct ccgatcagcc
      121 ggacctgcgg cgcatcctga cggattacgg gttcgagggg catccgctgc gcaaggactt
      181 cccgctgacc ggctatgtcg aggtgcggta cgacgaggac cgcaaggcga tggtctacga
      241 gccggtgcac ctgacgcagg acttccgcac gtttgatttc atgtcgccgt gggaggcgct
      301 gacgacgttg ccgggtgacg agaaggtgca cacggagcgg gccgaggaag gcagcaagcc
      361 atgagcgacg tcgaagcccg cccgaccgcg accaaggttg tcgagatcga cagccatgcg
      421 atcaatttcg gcccgcagca tccggccgcg catggtgtgc tgcggctgat cctggagatg
      481 gacggcgagg tggtggagcg cgccgatccg catatcgggc tgctgcatcg cggcaccgag
      541 aaattgatcg aatacaaaac gtatatgcag gcggtgccgt atttcgaccg gctcgattac
      601 gtctcgccga tgtgcgagga gcatgcgttc gcgctggcga ccgagaagct gctgggcatc
      661 gaggcgccgg agcgggcgca atggatacgc gtgctgttcg ccgagatcac ccgcgtactg
      721 aaccacctgc tgaacgtgac gacctatgcg ctggatgttg gggcgatcac gccgagcctg
      781 tgggggttcg aggagcgcga gaagctgatg gagttccatg aggccgcgtc cggtgcgcgg
      841 ctgcatgcga actatttccg tccgggcggt gtgtcgaagg atttgccggc ggggctggag
      901 cagcggatcg cggcgtgggc ggagacgttc ccgaagttcg tcgatgacct ggaagagctg
      961 ctgaccggca atcgcatctg gaagcagcgc accgtcgata tcggcgtgat ggatgcgaag
     1021 caggcgctgg cctgggggtt cacgggccca tgtctgcgcg cgagcggcgt gccgtgggat
     1081 ctgcggcgct cgcagccgta cgacaaatat gcggaggtcg atttccagat tccggtcggt
     1141 cgtcatggcg attgttatga ccgctatctg atccggctgg cggaaatgcg tgagagcgtg
     1201 aagatcgtca agcaggcgct ggcgaagatg cggcctgggc cgatcaaggt gcaggatcgg
     1261 aagttcacgc cgccgcaccg tgcggagatg aagcgctcga tggaggcgct gatccatcac
     1321 ttcaagctgt ataccgaggg ctatcacgtg cccgccggcg ccacctatac cgtggtggaa
     1381 agcccgaagg gtgagttcgg tgtgtacctg gtggcggacg gcaccaacaa gccgtatcgc
     1441 tgcaagatcc gctcgaccgg cttcgcgttc ctgcaggcga cagaggtgat ggcgaggcgc
     1501 cacatgctgg cggataccgt ggcgatcatc ggatcgctcg acatcgtttt tggcgagatc
     1561 gatcgatgag cgatttcgtg cagccggaac actttgcgtt cgatgcggag agcgaggcat
     1621 caattccgcc tctgctggcc aaatatcccg cgggcaggca ggcgagcgcg gtgatcccga
     1681 tgctgtatct ggtgcagaac cagatgaagc ggcagacggg cagcgcatgg gtgccgacgg
     1741 cgggtatgaa cgaggtggcg cggcggttgg acatgccgcc gatccgtgta tacgaggtcg
     1801 cgacgttcta tttcatgttc aacctgaagc cggtcgggaa gtggcatctg caggtctgca
     1861 cgacgacgcc gtgctggctg cgcggttcgg atgaagtgac cgcaacatgc cgcgcagtga
     1921 cgggcatcaa ggactggggc gagaccagcg ctgatggtct gttcaccatg acggaagtcg
     1981 agtgcgtcgg cgcatgcgtc aatgcgccgg tggtccaggt gaacgacgat ctgtacgagg
     2041 acctggatgc cgaaaccacg aagcagctgc tggatgcgct gcggcgggat gaaccgccga
     2101 aggtgggctc gatgaagggg cggcacacct cggcgccgga aggcgggccg aataccctga
     2161 ccacgttgca atttggtgcg gagagttgat ccgatgttag aggacaagga ccgcatcttc
     2221 accaatctct atgggctgca tgatccctat ctgaagggcg cgcgggcacg gggcgattgg
     2281 gatggcacga aggctattct tgagaagggc cgcgatgtca tcgtggagga agtgaaggca
     2341 tcggggctac ggggccgtgg tggggcgggt tttcccaccg gcctgaaatg gtcgttcatg
     2401 ccgaagcagt cggatcggcc gtgctatctg atcgtgaacg ccgacgagag cgagcctggc
     2461 acctgcaagg atcgcgacat catgcggcac gatccgcaca agctggtgga aggctgcctg
     2521 gttgccggtt tcgcgatggg tgcttgtgca gcgtatatat acatccgcgg cgagtttgcc
     2581 aacgaggcga aggtactgca ggcagcgatc gatgaggcgt acgaggcggg gctgatcggc
     2641 aaggatgcgt gcgggtcggg ctactcgttc gatgtgtatc tgcatcgcgg cgccggtgcg
     2701 tatatctgcg gcgaagagac cgggctgctg gagagcctgg agggcaagaa ggggatgccg
     2761 cggctgaagc cgccgttccc tgcggcggtt ggtctatacg gttgtccatc cacggtgaac
     2821 aacgtcgaga gcatcgcagt ggcgccgacg attatgcggc gcggtgctgc gtggttcaat
     2881 tcgctggggc ggccgaagaa ctccgggccg aaggtgttct gtatttcggg gcacgtgaac
     2941 aagccgtgca atgtcgagga agagctgggc attccgttgc gcgagctgat cgacaaatac
     3001 gcgggcggcg tgcgcggcgg ttgggacaat ctgctggcgg tgatcccggg cggggcgtcg
     3061 gtgccggtac tgccgaagag catctgcgac acggtgctga tggatttcga ttcgctgcgc
     3121 gaggcgcgca gcgggttggg gaccgcggcg gtgatcgtga tggacaagtc caccgacatc
     3181 atcaaggcga tcgcgcggct gtcgaagttc tacatgcatg agagctgcgg acagtgcacg
     3241 ccttgccggg aaggcaccgg ctggatgtgg cgcgtgatga cgcgcatggt ggagggaagg
     3301 gcggaagtcc cggaaatcga tacgctggag caggtcacgc ggcaggtcga aggacacacg
     3361 atctgtgcgc tgggcgatgc ggcggcatgg ccgatccagg ggctgatccg gcatttccgg
     3421 cccgagatgg aagcgcggat c
//
LOCUS       DATGEM010000036        10143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_332360, whole genome shotgun sequence.
ACCESSION   DATGEM010000036 DATGEM010000000
VERSION     DATGEM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332360"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..544)
                     /locus_tag="VKQ27_00930"
     CDS             complement(35..544)
                     /locus_tag="VKQ27_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLY87521.1"
                     /translation="MQQPRRTRLTEADRALWAIYTGSVRPMRGRAPIPARREQIVVSP
                     DAQRPAAPALVVRVPLAPLSVGAQPGGVDTASWQRLRTGKLSPTRTLDLHGHTAQRAH
                     RALSSFLRTAQAEGVRCVEIVTGQGAVLRRELMLWLNQPEFRGLVLAAAHPHAANPGA
                     VRLLLRRTR"
     gene            complement(517..1701)
                     /locus_tag="VKQ27_00935"
     CDS             complement(517..1701)
                     /locus_tag="VKQ27_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87522.1"
                     /translation="MPLWPLIATLAAQTLATMALFSLPAIAPAVARALDVPGELVGVF
                     VSVAYGVGIVSALLSPGFIHRYGGVRVLQGVLVATAAMLALAAGGSVMWLAIAAVVLG
                     LGYGAAAPASTHLLVPHTPPAVFNMVMSLRQIGVPLGGVLGSLLLPPLVVHVGWREAL
                     LAELPGVLLLTVLMEIPRRRWDVDVHPTRRLLGRALLAPFALLRDWPMLRLSIASFFY
                     SGTQLCFVAFTTTQLTTVAGFGLIEAGRALAIYQITGAVTRPIWGWIADRWLTPSHML
                     AVHGFGMAAASVAAGLFSPRWSHPAVLLVAAVAGSTGGGYTGVAYAEYAHLGGARRTE
                     ATGLGTAVMFAGVLLIPSSFGVTVAATGGYAFAYSVLAALALVSAVLLCLDTCSSRAA
                     PV"
     gene            1990..3264
                     /locus_tag="VKQ27_00940"
     CDS             1990..3264
                     /locus_tag="VKQ27_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87523.1"
                     /translation="MATIAQTVPWWREPTNEQWHAWIAAWLGWTLDAFDFTIFLLIML
                     PIAQSFNVPLTAVTAVFTLTLWMRLVGATAAGWLSDRIGRKKPLMISILWYSICNLLA
                     GLAPSFGLLFLFRALLGIGMGAEWPCGAALAMETWPQRSRGLMAGILQASWGIGALLS
                     SLAYALLYDAIGWRGLLVLGVAPALAVVYIRRYVREPELWAQNRAIQREQQREVRVPL
                     LNIFKRDVLGNTLNACWWMASGFIVAYSIGSLFPTHLQKDLGLSPSMVALPVMLQSFL
                     FFVSGFIWGWVADNLGRRWALILPAIGGMILTPLYLFTQDYTMIVVFFGLQGMFAGGG
                     MYATNPSYLAERFPTEVRATAAGFCYHQGAIFGGLTAPIIAYFAINWHIGFAIPMAIG
                     TLAGAVSFIIALALGPETRGKHLSAELGVATS"
     gene            complement(3327..4496)
                     /locus_tag="VKQ27_00945"
     CDS             complement(3327..4496)
                     /locus_tag="VKQ27_00945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87524.1"
                     /translation="MKAWHFSENAYPYLPPADEYESIRVSLPNRIYDPEKGAALYDRF
                     LDEWLIAEDEGMEIMLNEHHQTATCVDPAAPLVLAALARLTSKSRLLILGNPIANRRQ
                     PVRVAEEMAMIDVLSRGRLEAGFVRGVPYEVLPANSNPVRMNERQWEALDLIVKAWTT
                     HDGPFSHEGRFFHYRAVNIWPRPYQQPHPPIWISTTSPSGALRVGAHGYVQATFLTGF
                     DGTRAIYDAYRRGWREAGRGADVPVHRLAYAALVYAAPNEADARAGAEKLLWYITSNK
                     VPPHIANPPGYVPVGASVAMMRGAEHPLSAFRKAATVDGAIEAGIMFAGTPDQVYRQM
                     KRLYDHVGGFGHLLIMGQAGFLEHDETVLGIRTFAREVYPRIKEEFPDAAVSGVA"
     gene            complement(4493..5278)
                     /locus_tag="VKQ27_00950"
     CDS             complement(4493..5278)
                     /locus_tag="VKQ27_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY87525.1"
                     /translation="MQRHVIAIAGVELELFEAGEGPPILFLHGANGFAPEHPYVAPLA
                     ARHRLIAPSHPGFGKSSLPDWIDTPDDIAYIHLELLDRLGLHQVDVIGCSLGGWIACE
                     LATKVPELVRRMVLVGPVGVKTGPVDRLDIPDIFAQPQDKLNALLFHDPARMRMDPSR
                     LTDEQLAISVRNRETLALLTWEPYMHNPKLPHRLHRIAAPALFVRGESDGLVSAPYIE
                     SYARLLPNARVATIAVAGHAPQLEQTDEFARMALAFLEAGDVR"
     gene            5327..6316
                     /locus_tag="VKQ27_00955"
     CDS             5327..6316
                     /locus_tag="VKQ27_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87526.1"
                     /translation="MSKLQLSIAIGDYDRVRPLVDGSVQMDGVDPVFMLLEPEEIFFR
                     AFRHADFDICELSMSSFTVKTAAGDCPYVGVPVFPSRAFRHNSIYIRGDHGIARPEDL
                     RGKRIGVPEYQVTANVWTRAVLEDEYGVRPSDVTWVRGGYDQPGRIEKISLNLPPDVR
                     IEDAPDGATISGMLAAGEIDAVIGPRAPSCFGHHPQVRWLFPDPAVAAADWFRKTRIF
                     PIMHILGIRRSLADRHPWLPFAALKAFEQAKARAMEKLATVAASKVTLPFIDEQLHAA
                     RRLMGDDFWSYGLEANRHVLEAFLRRHHVEGLSARVLQPEELFHPSTREAHRI"
     gene            complement(6410..7858)
                     /gene="amrB"
                     /locus_tag="VKQ27_00960"
     CDS             complement(6410..7858)
                     /gene="amrB"
                     /locus_tag="VKQ27_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HLY87527.1"
                     /translation="MMNAIRQPAVAGTFYPAQRDVLERQLTQFLSEAANDAPAAASPK
                     AIIGPHAGYIYSGSVAARAYARLAGARGRIKRVVLIGPSHYIAFRGIAVDASEAWAMP
                     SGTVTIDREAFDLLRAMPMVGVLDAAHEREHALEVHVPFLQQVLGDFRLVPIVTGDAP
                     PEAVAAVIEALWGGPETLIVISTDLSHYLDYAACQQIDQATADAIERFDPDVLTPTHA
                     CGSLATKGLLRAAKARGMAIERLDLRNSGDTAGPRDRVVGYGAWALYERGAVDDESAK
                     IDAIGPMLIDLARRSILFGLDTGFPPQVITDASLPPVLAAPGAAFVTLRRPDGSLRGC
                     IGSVIATRPLAVDVVQHAFNSAFRDWRFPRLSWLELAGLSLSVSVLTPPTPMQFEGEA
                     DLLAQLQPRIDGVIIEDMGLRSLFLPSVWEEIPDKRHFVTALKLKAGMQADHFSPQFQ
                     AQRFRSIEVKGAMEAGLGQSNGPLGWKVLPPS"
     gene            7857..8963
                     /gene="amrS"
                     /locus_tag="VKQ27_00965"
     CDS             7857..8963
                     /gene="amrS"
                     /locus_tag="VKQ27_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HLY87528.1"
                     /translation="MPRLPHMETALEGTLFPGRYWHSLPDGRVQCDVCPRFCKLHEGQ
                     RGLCFVRARQDDAIVLTTYGRSSGFCIDPIEKKPLNHFLPGTPVFSFGTAGCNLTCKF
                     CQNYDISKAREVDRLNQDATPQMIAAAARQLGCRSVAFTYNDPVIFLEYAVDTARACH
                     ELGIKTVAVTAGYITPDARREFYAHMDAANVDLKGFTEPFYRNLCGGRLADVLDTLVW
                     IKANTNVWLEVTTLLIPGENDSDAEIGALSAWFAAELGRDVPLHFSAFHPDWRMREHP
                     ATPPATLTRARAIAMSHGLRYVYTGNVHDSAGGSTYCHACGSLLIGRDWYELTGWDLD
                     ANGCCRRCGAAAAGVFEPTHGTWGRKRQPIQFRC"
     gene            complement(8965..9951)
                     /locus_tag="VKQ27_00970"
     CDS             complement(8965..9951)
                     /locus_tag="VKQ27_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87529.1"
                     /translation="MSRLRVAIRVPVGRPLPEVAAFVARCEDAGFDGVGIHDHPSSGR
                     DAYLALALAAQQTQRLRLFPATSSPLVRHPLVLASLAHSLEEIAPGRALLTVAPGFIS
                     TRSIGQPRAGVAVMREAIGDLRRLLIGEPVAFGQTSTKLRNLSATPTPVYLLAAGPRM
                     IELAGEVADGVVLFVGLHSASIRAAMRHLQEGANRTGRSLDGFPVIFAVTLGLGDEDV
                     GARWVRSWFAPGQPFLEYPSAANLRWLHEAGFDVARGQDPATVAEARHIADAFGLFGS
                     PERCADRLLQVHGDAGVKDVFLFPAHDLPGGYTMPETEVRAFEQVIRPRLGR"
BASE COUNT         1803 a         3231 c         3351 g         1758 t
ORIGIN      
        1 gctcgaaggg cccgaagcct ggctccgaat ggcgtcaccg ggtccgcctg agcaggaggc
       61 gtaccgcgcc gggattggca gcgtggggat gtgccgccgc caaaaccagt ccacgaaact
      121 ccggctggtt cagccagagc atcagttctc gccgcaacac ggcgccctgg ccggtgacga
      181 tctcgacgca tcgcacgcct tcggcctgtg cggtgcgcag gaagctggac aacgcgcgat
      241 gggcgcgctg ggcggtatgg ccatgaagat cgagcgtccg ggtgggcgat agcttgccgg
      301 tgcggaggcg ctgccacgaa gcggtgtcca ccccgcctgg ctgcgcgccc acggacagcg
      361 gcgccagcgg aacccgcacc actagggctg gagccgccgg gcgctgggca tccggcgaca
      421 cgacgatctg ctcccgacgt gccggtattg gcgcccggcc acgcatgggc cggacagacc
      481 ccgtgtagat cgcccagagc gcgcggtcgg cctcggtcag acgggtgcgg cgcggctgct
      541 gcatgtgtcg agacagagca gaacggcgct gaccaaggca agggcggcaa gcaccgagta
      601 ggcaaaagca tagccgccgg ttgccgccac ggtgacaccg aagctcgagg ggatcagcag
      661 cacgccggcg aacatcaccg cggtgccgag gcctgtcgcc tcggtccgtc gcgcaccgcc
      721 gaggtgagcg tattcggcat aggcgacgcc ggtgtaaccg ccgccggtgc tgccggcgac
      781 ggcggcgacg agcagcaccg cgggatgcga ccagcgcggg ctgaacaagc cggcggctac
      841 cgatgcggcc gccataccga agccatgcac ggcgagcatg tgacttggcg tcagccagcg
      901 atccgcgatc catccccaga ttggccgggt gaccgcgcct gtgatctggt agattgcgag
      961 cgcgcgccct gcctcgatca gaccgaaacc cgccaccgtg gtgagttgcg tcgtggtgaa
     1021 ggcgacgaag cacagttgtg tgccggaata gaagaagctg gcgatggaca gtcgcaacat
     1081 cggccagtcg cgcagcagag cgaacggcgc cagcagggcg cggccgagca gccggcgagt
     1141 gggatgcacg tcgacgtccc agcggcgtcg cgggatttcc atcagcacag tgagcagcag
     1201 cacgccaggc agctcagcca gcagcgcctc gcgccagccg acgtgcacca ccagcggcgg
     1261 caggagcaac gagccgagta cgccgcccag cggcacgccg atctggcgga gcgacatcac
     1321 catgttgaac actgccgggg gcgtatgcgg gaccagcaga tgcgtggagg ccggcgccgc
     1381 ggcgccatag ccgaggccca gcaccacggc ggcgatcgcc agccacatca cgctgccgcc
     1441 ggcggccagc gccagcatgg ccgcagttgc gacaagcaca ccctgcagca cacgcacgcc
     1501 gccgtagcgg tggatgaatc cgggcgagag cagtgccgat acgataccga caccatatgc
     1561 gacggagacg aacacgccga cgagttcgcc cggcacatcg agggcccgcg ccaccgccgg
     1621 ggcaatcgcg ggcaaggaga agagtgccat ggtcgccagc gtttgcgctg ccagcgtggc
     1681 gatcaaaggc cagaggggca tgatgcaggg atactgccct atcagggatg gttgtgcgag
     1741 gtacggccac agacatcacg gctcaccccg ccgtgtgtcg aggaatacta tgacatttct
     1801 gtatcgtaat atttatagtc ggtattcatc gaaaacgccg cagtaaacgg cgcttggcca
     1861 tcccttgcgc gcgctgcgct gcaccaaaat accttcgaat gcaagtaaag tttccgcgac
     1921 gaacgcggcg cggcgcgcaa ttcagctatg ctttcggtga agcttcacac ccaagggaag
     1981 gagttggcga tggcgacgat cgcacagacg gttccgtggt ggagagagcc gaccaacgaa
     2041 caatggcacg cctggattgc agcgtggctg ggatggacgc tcgatgcgtt cgatttcacc
     2101 atcttcctgt tgatcatgtt gccgatcgcg cagtcgttca acgtaccgct cactgcggtc
     2161 actgccgtgt tcaccctcac actttggatg cggctggtcg gtgccaccgc ggccggctgg
     2221 ctgtctgatc gcatcggtcg caagaagccg ctgatgatct cgatcctgtg gtattccatc
     2281 tgcaacctgc tggcggggct ggcacccagt ttcggcctgc tgtttctgtt ccgcgcgctg
     2341 ctcggtatcg ggatgggggc cgagtggcca tgtggtgcag cattggccat ggagacgtgg
     2401 ccacagcgct cacgcggtct catggcaggg atcctgcaag cgtcgtgggg catcggcgcc
     2461 ctgctatcca gtctcgcgta tgccttgttg tatgacgcga ttggctggcg tggcctgttg
     2521 gtccttggcg tcgcgccggc actggctgtc gtctacattc gccgttacgt cagggagcca
     2581 gagctctggg cacagaaccg ggcgatccag cgggagcaac agcgcgaagt ccgggtgcca
     2641 ctgctcaaca tcttcaagcg ggatgtcctt ggcaacacgt tgaatgcctg ctggtggatg
     2701 gcaagtggct ttatagtcgc ctattcgatc ggctcactgt ttccgaccca tctgcaaaag
     2761 gacctgggct tgagtccgag catggtggcg ttgccggtta tgctgcaaag cttcctgttc
     2821 ttcgtgtccg gcttcatctg gggctgggtt gccgataacc tcggccggcg ttgggcactg
     2881 atcctacccg ccattggcgg catgatcctg acgccgctct atctgttcac ccaggactac
     2941 acgatgatcg tggtgttctt cggcctgcaa ggcatgtttg ccggcggcgg catgtatgca
     3001 acgaatccca gttacctggc cgaacggttc cccacagaag tgcgcgcaac ggcagccggg
     3061 ttctgctacc atcaaggcgc gatcttcggt ggattgaccg cgccaatcat cgcttacttc
     3121 gcgatcaact ggcatatcgg ctttgccatc ccgatggcga ttggcacgtt agcgggcgcc
     3181 gtgagcttca tcattgcgct ggcccttggc cccgagacgc gtggcaagca cctttctgcc
     3241 gagcttggtg tggcgacgag ctgagacgtg gatgtcggcg ggcgcatcga tcatcgatac
     3301 gcttcgccga ctttgcgagc accggactac gccacgcccg agaccgccgc gtcggggaat
     3361 tcctccttga tgcgtggata gacctcgcgc gcgaaggtgc ggatgccgag caccgtctcg
     3421 tcatgctcca ggaagccggc ctggcccatg atcagcaggt ggccaaagcc tccgacatgg
     3481 tcgtacaggc gcttcatctg gcgatacacc tggtctggcg tgccggcgaa catgatgccg
     3541 gcctcgatcg cgccatcgac cgtggcggcc ttgcggaacg cgctgagcgg gtgctcggcg
     3601 ccgcgcatca tcgcgacgga tgcaccgacg ggcacgtagc cgggcgggtt ggcgatatgc
     3661 ggcggtactt tgttcgaggt gatgtaccac agcagcttct ccgcgcccgc gcgcgcgtcg
     3721 gcctcgttcg gtgcggcata gaccagagcc gcataggcca acctatgcac cggtacgtcc
     3781 gcgccccgcc ccgcttcacg ccagccgcgg cgataggcgt cgtagatcgc gcgcgtgcca
     3841 tcgaatccgg tgaggaacgt cgcctgcacg tagccatgcg ccccgacgcg tagcgcccca
     3901 gaggggctgg tggtgctgat ccaaatcggc ggatgcggct gctggtaggg acgcggccag
     3961 atgttcaccg cgcggtagtg gaagaagcga ccctcgtggc tgaacgggcc atcatgcgtg
     4021 gtccaggcct tcacgatcag gtcgagcgcc tcccactgcc gctcgttcat gcgcacgggg
     4081 ttgctgttgg cgggcagcac ttcgtatggc acgccgcgga cgaacccagc ctccagccgg
     4141 ccgcgcgaca gcacatcgat catcgccatc tcttccgcca cgcgcaccgg ctggcgacgg
     4201 ttggcgatcg gattgccgag gatcagcagc cgcgacttgc tggtgagccg ggcgagcgca
     4261 gccagcacca gcggcgcggc gggatcgacg caggttgccg tctgatggtg ctcgttgagc
     4321 atgatctcca tgccctcgtc ctcggcgatc agccattcgt cgaggaaacg atcgtacagc
     4381 gcggcgccct tctcggggtc gtagatacgg ttcggcaagg acacgcggat cgactcgtac
     4441 tcgtctgccg gcggcaggta ggggtaggcg ttctcgctga aatgccaggc cttcatcgca
     4501 catctcctgc ttccaggaac gccagtgcca ttcgggcgaa ttcatctgtc tgttccagtt
     4561 gcggcgcgtg gccagccacg gcgatcgtag caacgcgcgc attcggcagc aggcgggcgt
     4621 acgactcgat atacggcgcc gagaccagac catcactttc gccgcgcacg aacagtgccg
     4681 gtgcggcaat acggtgcagg cgatgcggca gcttcggatt gtgcatgtac ggttcccatg
     4741 tcagcagcgc cagcgtttcg cgattgcgca ccgaaatcgc cagctgctca tcggtcagcc
     4801 gcgacgggtc catgcgcatg cgggcaggat cgtggaacag cagcgcattc agcttgtcct
     4861 gcggctgcgc aaaaatgtcc ggaatgtcga ggcgatcgac cggtcctgtc ttcaccccca
     4921 ccggccccac gaggaccata cggcgcacaa gctccgggac tttggtggcg agttcgcatg
     4981 caatccagcc cccgagcgag cagccgatca catccacctg atgcaggcca agtcgatcca
     5041 gcaactccag atggatgtag gcgatatcgt ccggcgtatc gatccagtcc ggcaggctgg
     5101 atttgccaaa gcccggatgc gatggcgcga tcagccgatg cctcgcggcg agcggggcca
     5161 catacggatg ctccggggcg aagccgttcg cgccgtgcag gaacaggatc ggggggcctt
     5221 cgccagcctc gaacagctcg agttcgacgc cggcgatcgc aatgacgtgc cgttgcaagg
     5281 gacgtcctcc tgtattttga atgagaggct atgccgggag ggaaccatga gcaaacttca
     5341 gctatccatc gccatcggcg actacgatcg agtccgtccg ctggtcgatg gcagcgtgca
     5401 gatggatggc gtcgatccgg tgttcatgct gctggagccg gaggagatct tcttccgtgc
     5461 attccgtcac gccgatttcg atatctgcga actgtcgatg agcagcttca ccgtaaagac
     5521 cgctgccggt gattgcccgt atgtcggcgt gccggtgttt ccatcgcgcg cgtttcgtca
     5581 caactcgatc tacatccgcg gcgaccatgg catcgcgcgg cctgaggacc tgaggggcaa
     5641 gcgtatcggc gtgccggaat atcaggtcac ggcaaatgta tggacacgcg cggtgctgga
     5701 ggatgagtac ggcgtaaggc cctcggacgt tacgtgggtg cgcggcggct acgatcaacc
     5761 ggggcgcatt gaaaagatct cgctgaacct gccgccagac gtccgcatcg aggatgcgcc
     5821 ggacggcgcc actatctccg gcatgctggc ggccggcgag atcgatgcgg tgatcggccc
     5881 gcgcgcgcct tcatgctttg gccaccatcc gcaggttcgc tggctgtttc ctgatccggc
     5941 ggtggccgcg gcggactggt ttcgcaagac acgtatcttt ccgatcatgc atatcctggg
     6001 cattcgccgt tcattggcgg accggcatcc ctggctgcct ttcgccgcgc tgaaggcgtt
     6061 cgagcaggca aaggcgcggg cgatggagaa gctggcaacg gtggcggcgt cgaaggtcac
     6121 cctgccgttc atcgatgaac aactgcatgc cgcgcggcgg ctgatgggcg acgacttctg
     6181 gtcgtacggc ctggaggcaa accgccacgt gctggaggcg ttcctgcgcc ggcatcatgt
     6241 ggaagggctg tcggcgcgcg tgctgcagcc ggaggagctg tttcatccgt cgacgcgtga
     6301 ggcgcatcgc atctaggagg tggatctgtg agtgcgtgga gctgatggac gaagctgtcc
     6361 cggtcgggat tgcttcgtcg gctacgcctc cttgcaatga cagagagggc taggaggggg
     6421 gcagcacttt ccatccgagc gggccattgc tttggccaag acccgcctcc atcgccccct
     6481 tcacctcgat ggaccggaac cgttgcgcct ggaactgcgg cgaaaaatgg tcggcttgca
     6541 tgccggcctt caatttcagc gccgtgacga agtggcgctt gtcggggatt tcctcccaca
     6601 cgctcgggag gaagagcgag cgaaggccca tgtcctcgat gatcacgccg tcgatgcgtg
     6661 gttgcagttg cgccagcaga tcggcctcgc cttcgaactg catcggcgtg ggcggcgtca
     6721 gcacagagac cgacagcgac agtccggcca gctccagcca gctcagccgc gggaaccgcc
     6781 agtcgcggaa tgccgaattg aaggcgtgct gcaccacatc gacggccagc ggccgcgtcg
     6841 caatgaccga cccgatacag ccacgcagcg aaccatcggg ccgccgcagg gtgacgaatg
     6901 cggcgcctgg cgcggcgagc accggcggca ggctggcatc cgttatcacc tgcggcggga
     6961 agccggtatc cagcccaaac aagatcgaac gccgcgccag gtcgatcagc atcggaccga
     7021 ttgcgtcgat cttcgcgctc tcgtcatcga cggcgccgcg ttcgtagaga gcccaggcgc
     7081 cgtagcctac gacacggtcg cgcgggccgg cggtgtcacc cgagttgcgc aggtcgagac
     7141 gctcgatcgc catgccacgt gctttcgcgg cgcgcagcag ccccttcgtc gccagcgagc
     7201 cgcacgcatg cgttggtgtg agcacgtcgg gatcgaagcg ctcgatggcg tcggcggtgg
     7261 cctggtcgat ctgctggcag gcggcgtagt ccagatagtg cgacagatcg gtggagatca
     7321 cgataagtgt ctccggcccg ccccacagcg cttcgatcac cgcggccacg gcttcgggcg
     7381 gcgcgtcgcc ggtgacgatg ggcacgaggc ggaaatcgcc cagcacctgc tggaggaacg
     7441 gcacgtgcac ttccagtgca tgctcgcgct cgtgtgcggc gtccagcacg cccaccatcg
     7501 gcattgcgcg cagcagatcg aatgcctcgc ggtcgatagt gacggtaccg ctgggcatcg
     7561 cccacgcctc cgacgcatcg acggcaatgc cgcggaacgc gatgtaatgt gatgggccaa
     7621 tcagtacaac gcgcttgatc ctgccgcgtg caccggcgag tcgggcgtag gcgcgagcag
     7681 cgacgctgcc cgaatagatg tagccggcgt gtggtccgat gatcgctttc ggtgacgcgg
     7741 cggcgggtgc gtcgttggcc gcctcggaca ggaactgcgt gagctgccgc tccagcacgt
     7801 cgcgctgcgc tggatagaag gttccggcca cggcaggctg gcggattgca ttcatcatgc
     7861 ccagattacc acacatggag acggcactag aaggcacact cttccccggc cgctattggc
     7921 attcgctacc cgacggccgc gtgcagtgcg acgtatgccc gcggttctgc aagctgcatg
     7981 aaggccagcg cgggctgtgc ttcgtccgcg cgcggcagga cgatgcgatc gtactgacca
     8041 cctatggccg gtcgtcgggc ttctgcatcg acccgatcga aaagaagccg cttaaccatt
     8101 tcctgcccgg cacgccggtg ttctccttcg gcaccgctgg gtgcaacctg acctgcaagt
     8161 tctgccagaa ctacgacatc agcaaggcgc gcgaggtcga tcggctgaac caggacgcca
     8221 caccgcagat gatcgccgcc gccgcgcgcc aactcggctg ccggtctgtt gcattcacct
     8281 acaacgatcc agtcattttc ctggaatatg ccgttgatac agcacgtgcc tgtcatgagc
     8341 ttggcatcaa gacggtcgcg gtgaccgccg gctacatcac gccggacgca cgccgcgaat
     8401 tctacgcgca tatggatgcg gcgaacgttg acctcaaggg ttttaccgag ccgttctatc
     8461 gcaatctttg tggcggacgg ctggccgatg tgctcgacac gcttgtatgg ataaaggcaa
     8521 atacgaacgt gtggttggaa gtcaccacgc tgctgattcc cggcgagaac gacagcgatg
     8581 ctgaaatcgg cgcgctatcg gcctggtttg ccgccgagct tggccgcgat gtgccgctgc
     8641 atttctcggc gttccatcct gattggcgca tgcgcgagca tccggccacg ccgccggcga
     8701 cgctcacccg cgcccgcgcg atcgcgatga gccatgggct gcgctacgtg tataccggca
     8761 atgtgcacga cagtgccgga ggcagcacct actgccacgc atgcggctcg ctgctgattg
     8821 gccgcgactg gtatgaactg accggctggg acctcgacgc gaacggatgc tgccgcaggt
     8881 gcggcgccgc ggctgccggc gtcttcgagc ccacgcatgg cacctggggg cgcaagcgcc
     8941 agccgatcca gttccggtgc tgagtcaccg gccgaggcgt gggcggatta cctgctcgaa
     9001 cgcgcggact tcggtttccg gcatggtgta gccgcccggc aaatcgtgag ccgggaacag
     9061 gaacacatct ttaacgccgg catctccgtg cacctgcaac agccgatccg cacaacgttc
     9121 cggcgatccg aacagcccga atgcatccgc aatgtgacgt gcttcagcga cagttgccgg
     9181 atcctgtcct cgcgcgacat cgaaccccgc ttcgtgcagc cagcggagat tggcggcgct
     9241 cggatactcc aggaacggct gccctggggc gaaccagctt cgtacccagc gcgctccgac
     9301 gtcttcgtcg ccgaggccca gcgttacggc gaagatgacc gggaagccat cgagtgaacg
     9361 gcccgtgcga ttggcacctt cctgcaagtg gcgcatcgcc gcgcggatcg aggcggaatg
     9421 cagcccgacg aacagcacca cgccatctgc cacttcaccg gccagttcga tcatccgcgg
     9481 gcctgccgcc agtagataca ccggcgttgg cgtagcactt agattgcgca acttcgtcga
     9541 tgtttgcccg aaggctaccg gctccccgat cagcaaccga cgcagatcgc cgatcgcttc
     9601 gcgcatcacg gcgacgcctg ctcgcggttg gccgatgctg cgcgtcgaga tgaaaccggg
     9661 cgcgactgtc agaagcgcgc gacctggggc gatctcttcc aggctgtgcg cgagcgatgc
     9721 cagcaccaga ggatgccgca ccagcgggct ggatgtggcc ggaaagagtc gcaatcgttg
     9781 ggtctgctgc gcggccaggg ccagggcgag ataggcatca cgcccgctgg atggatggtc
     9841 gtggataccg acgccgtcga agccggcatc ctcgcaccgc gcgacgaatg ccgcgacctc
     9901 cggcagtggc cggcccacgg gaacgcggat cgctacgcgc agtcgggaca tgctggatct
     9961 ctccatccaa tgcggcacgg tagcgcgcgc ccggcctgcg acatatcctg gcccgggcgg
    10021 gacgaggaga gttgaccaca tgcatgccgg tgaacggctc gctgaattcg tcgcgacgtc
    10081 gcaatggggc tctgtgccgg aacaggtccg gcatgagggc aagcggtcgc tgctgaactt
    10141 tct
//
LOCUS       DATGEM010000037         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_785541, whole genome shotgun sequence.
ACCESSION   DATGEM010000037 DATGEM010000000
VERSION     DATGEM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785541"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..760
                     /locus_tag="VKQ27_00975"
     CDS             152..760
                     /locus_tag="VKQ27_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87530.1"
                     /translation="MMALLEAGMGIIPCYEPSLFPDQCIVRADRNDTIVFPHVDSCMA
                     VTVFQGRGEKFWAIGGHCPMFDEAPELSAAGTLVRFCERMCEKESIRASQIEKIIFVG
                     GNKRKKLTQYEHYSISQALAVFNLSKQSNAANWAIVNVRNCENGVDVFVDLPKQRLSI
                     QQFRKDPSLEAFKPPPLRPDAFYSEAIHTIVGKIIRPPDSFF"
     gene            complement(781..2226)
                     /locus_tag="VKQ27_00980"
     CDS             complement(781..2226)
                     /locus_tag="VKQ27_00980"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY87531.1"
                     /translation="MDPTFLPAWRLAELVRGGAIGCVELLDHFIARVEVLDGKTNAVV
                     VRDFERAREQARTLDKQRAAGRAAPLLGVPMTVKESFGVAGLRSTWGFEEHRNHVAHE
                     DALAVKRLKAVGAVIFGKTNVPVGLQDWQSYNPIYGTTNNPWDAGHTPGGSSGGGAAA
                     TAAGLTGLEIGSDIGGSVRVPAHYCGVFGHKPTWGLCPTRGHALVVSAAMTDISVIGP
                     LARSAADLTLVLDAIAGPDEAETAAHAPLPPPRAMRLSDLRVAVWSSEKGQETDDEVT
                     AAIEAVADFLESKGAKVSRIARPEFDATEAYHIYLKLLATALSARHSEETVQLWREGK
                     AHYPADDMSANAIQVRTVDMTHREWLRLNERRFQLRRAWGAFFHEWDVLLCPPIATAA
                     LPHRQDGETWERRITINGREVAYNDMLFWPGVTCGFHLPASVAPIGLTKAGLPIGVQI
                     VGPLYGDRTTIHVAGLLEQHWRQFVAPPGWG"
     gene            complement(2226..>2647)
                     /locus_tag="VKQ27_00985"
     CDS             complement(2226..>2647)
                     /locus_tag="VKQ27_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87532.1"
                     /translation="AVIDGMIERKFGRIVNITSASVKSPIPELGMSNGARAGLTGFVA
                     GLARQVAKHNVIINNILPGPFLTDRLRSGFQEAAKRTNRSVDEVVADRLQTNPAGRAG
                     DPAEFGEACAFLCSAKVSYIVGQNLLLDGGAFNSSST"
BASE COUNT          493 a          956 c          732 g          466 t
ORIGIN      
        1 gacaaagtca ttactgagct tcgatcgtcg acgcgattac agcctccggc gatgccggcg
       61 ccacatgatt tatataagtc tcagttattt cggccctttc cgggctgatg atgcaatcaa
      121 tacttgaact atcattttgg atttgcttat tatgatggcg ttattggagg ccggcatggg
      181 catcataccg tgttacgaac cttccctgtt tcccgaccaa tgcatcgtgc gggccgatcg
      241 caatgacacg atcgtttttc cgcacgtcga tagttgcatg gcggtcaccg tcttccaggg
      301 gcgtggcgag aagttctggg cgatcggcgg gcattgcccc atgttcgatg aggcaccgga
      361 actgtcggcc gccggcaccc tcgtgcgctt ctgcgagcgg atgtgcgaga aggagagtat
      421 tcgcgccagc cagatcgaga agatcatctt cgtcggcggc aacaaacgca agaagctcac
      481 ccagtacgag cactattcca tcagtcaggc actcgcggtc ttcaatctta gcaagcagag
      541 caatgcggcc aactgggcca tcgtgaacgt gcgcaactgc gaaaatgggg tcgatgtctt
      601 cgtggatctt ccgaagcaac gactatccat tcaacaattc agaaaagacc cgagcctgga
      661 ggcgttcaag ccgccaccgt tgcgtcccga cgcgttctac agcgaagcga tccatacaat
      721 tgttggcaaa atcatcagac ccccagactc atttttctga ggcagggtga caaaagcccg
      781 tcacccccac ccgggcggcg ccacgaactg ccgccaatgc tgctccagca gtcccgcgac
      841 atggatcgtc gtccggtcac catacagcgg cccgacgatc tgcaccccga tcggcaaccc
      901 cgccttcgtc aacccgatcg gtgccacgct ggccggcaga tggaatccgc acgtcacgcc
      961 aggccagaac agcatgtcgt tgtacgcgac ttcacgcccg ttgatcgtga tccgccgctc
     1021 ccacgtctcg ccatcctgcc gatgcggcaa cgcagccgtc gcgataggcg gacacagcag
     1081 cacatcccac tcatggaaga acgcgcccca cgcgcgccga agctggaacc ggcgctcgtt
     1141 cagccgcagc cactcgcgat gcgtcatatc gacggtgcgc acctgtatcg cattggcgct
     1201 catatcgtcc gccggataat gcgccttgcc ctcccgccag agctgcaccg tctcctcgct
     1261 gtgccgcgcg ctcagcgccg tcgccagaag cttcaggtag atatggtatg cctcggtcgc
     1321 atcgaactcc gggcgcgcga tgcggctcac cttggcgccc ttgctctcca ggaaatcggc
     1381 caccgcctcg atcgcagcgg tgacctcatc gtccgtctcc tgccccttct cgctcgacca
     1441 aacggcaacc cgcagatcac tcagccgcat cgcccgcggc ggcggcagag gcgcgtgcgc
     1501 cgccgtctct gcctcatccg gcccggcgat cgcatccagc accagcgtca gatcggcagc
     1561 cgagcgcgcc agcggcccga tcaccgagat atcggtcatc gccgcactga ccaccagcgc
     1621 atggccgcgt gtcgggcaca gcccccacgt cggcttatgc ccgaacaccc cgcaataatg
     1681 cgccggcaca cgcacagaac cgccgatgtc gctgccgatc tccagcccgg tcagcccggc
     1741 cgccgtcgcc gccgcaccgc cgccagacga cccccccggc gtatgccccg catcccacgg
     1801 attgttcgtc gtgccgtaga tcgggttgta gctctgccag tcctgcagcc ccaccggcac
     1861 gttggtcttc ccaaagatca ccgcgccgac cgccttcagc cgcttcaccg ccagcgcgtc
     1921 ctcatgcgcg acgtgattgc gatgctcctc gaagccccag gtactgcgca gcccggcaac
     1981 gccaaagctt tccttcaccg tcatcggcac gccaagcagc ggcgcggcac gccccgccgc
     2041 ccgctgcttg tccagcgtcc gtgcctgctc gcgcgcacgc tcgaagtcgc gcaccaccac
     2101 cgcgttggtc ttgccgtcga gcacctcgac ccgcgcgatg aagtggtcca gcagctcgac
     2161 gcagccgatg gcgccgccgc ggaccaactc cgcgaggcgc catgccggaa ggaaggtcgg
     2221 atccatcagg tgctggacga gttgaacgcg ccgccatcca gcagcaggtt ctgcccgacg
     2281 atgtagctga ccttcgccga acacaggaac gcgcaagcct cgccgaactc ggccggatcg
     2341 cccgcgcgcc ccgccggatt ggtctgcagc cgatcggcca cgacctcatc gaccgaacga
     2401 ttggtccgct tcgccgcctc ctggaagccg ctgcgcagcc gatcggtcag gaatggcccc
     2461 ggcaggatgt tgttgatgat gacgttgtgc ttcgccacct gccgcgccag gcccgccacg
     2521 aaccccgtca gtcccgcgcg cgcgccattg ctcatgccaa gctcggggat cggcgacttc
     2581 accgacgcgg aagtaatatt gacgatccgc ccgaacttcc gctcgatcat cccatcgatc
     2641 accgcct
//
LOCUS       DATGEM010000038         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1357052, whole genome shotgun sequence.
ACCESSION   DATGEM010000038 DATGEM010000000
VERSION     DATGEM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357052"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VKQ27_00990"
     CDS             complement(<1..1315)
                     /locus_tag="VKQ27_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY87533.1"
                     /translation="MSANYAAAAPAPALTGGRKAITAAALALGTFMQVLDTSIANVSI
                     PTIAGNLGVSADQGTWVITSFAVANGVSVPLTGWLMQRFGVVRTFVISVALFTIASLL
                     CGLAWSLPSLIAFRVLQGAVSGPMIPGSQALLIEVFGPSKRNAALAVWSLTTLVAPIA
                     GPLLGGYISDNYVWPWIFLINVPVGVFCTLLCWNNLKSRETPTRKLPIDRVGLILLFV
                     WVGALQILLDKGKDLDWFNSPVIVALALITAVAFAAWLIWELTEQHPIVDLSLFRSRS
                     FSLGTAALCLGYAVFFGNIVLMPLWLQSFLGYTATWAGLVSAPSGVTAVLTSLMIGRL
                     MRRFDPRLLAATSFGLYAVSYFMRAGLTADAGFIAYMAPQLVQGFAMGLFFVCMLAVI
                     FDGLPPNKIPAASGLSNFLRITAGSFATSLTTTFWDRREALHQARL"
     gene            complement(1312..2607)
                     /locus_tag="VKQ27_00995"
     CDS             complement(1312..2607)
                     /locus_tag="VKQ27_00995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLY87534.1"
                     /translation="MSTPELSVTGGSKVTSESELPSEPNSPQPAGRSRRLGLFGLGAV
                     VVLGGFGYLGYWFLDGRYYESTDDAYVNGDVVQVTSEIPGTVIALNVDDTQAVHAGQP
                     LLQLDPADAKIAAANAEADLARAVRQVRGLFAQGKELRAQIDQREQAERTADEDLRRR
                     GGLIADGAISAEELSHARDAVTTTRANVAAARQQLSQTVAQIDGTTIADHPQVLAAAA
                     AVRNAALALHRTALSSPVTGLIAKRSVQVGQRVAAGSPLLAVVPLDDVWIDANFKEGQ
                     LERMRAGQPVTVRTDLYGRGVTYHGHVVGIAAGSGNAFALLPSQNASGNWIKIVQRLP
                     VRILLDPQELKAHPLRVGLSTNVRVDLHDTSGPLMTMAVRNEPQPTQPSAGDDPAVDG
                     RIAEIIADNAGPKAHEPARLSRKNKVGTESGSTVLAAGS"
     gene            complement(2604..>3944)
                     /locus_tag="VKQ27_01000"
     CDS             complement(2604..>3944)
                     /locus_tag="VKQ27_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLY87535.1"
                     /translation="AEPAAEGWWDSFQDPQLDRLIRLGLKDSPTLTEAQARVSGALAQ
                     AQAAQAGLRPSVNLDASALYQRAPQNYVIPPPLAGHSFWMGQAGASLGWDLDFWGRQA
                     DAVRRAQNLVQSARFDVDYARLMLAGAITQAYVELYREYALVDIAERSEAQRQNIVDI
                     TRRRVAAGLDTQLELREAEGQLPQARVARAQAQAAAELAVHQLAQLTGQGAESYASIQ
                     RPVLHVEAALPVPRALPINLLARRPDVLSSRLTVEAADAERLAAKAAFYPDVSLRALA
                     GIGAFGMSNLVQWSARGYGAGPLISLPLFDGGRLRAEYQGSEAQLDAAIAGYNDTVLR
                     AVQQTGDQISNLDALDRERLDQQQTLEATEAAYRLAEERYRAGLASYLSVLNAETQVL
                     TARRSMVDILTSQVVSRVTLLLAVGGSFDPRASGALADASHTDTSSGTLTRTNP"
BASE COUNT          710 a         1264 c         1328 g          642 t
ORIGIN      
        1 ccagccgtgc ctgatgcagc gcttcgcgcc ggtcccagaa ggtcgtggtc agcgaggtgg
       61 cgaagctgcc tgccgtgatg cgcaggaaat tcgacaagcc ggacgccgcg ggtatcttgt
      121 tcggggggag accatcgaag atcacggcaa gcatgcagac gaagaacaat cccatggcga
      181 atccctgcac cagttgcggc gccatgtagg cgatgaagcc ggcatcggca gtgagcccgg
      241 cgcgcatgaa ataggagacg gcgtaaaggc cgaacgaggt tgcggccagc aggcgcggat
      301 cgaagcggcg catcaggcgc ccgatcatga gcgaggtcag caccgcggtc acaccgctcg
      361 gagcggagac caagccggcc caggtggccg tgtagcccaa aaagctttgc agccacaagg
      421 gcatgaggac gatattgccg aagaaaacgg cgtatcccaa gcacagtgcc gcggtgccga
      481 gcgaaaagct gcgcgacctg aagaggctca agtcgacgat gggatgctgt tcggtcagct
      541 cccagatgag ccacgcggcg aaggcaacgg cggtgatgag agccagggcc acgatgaccg
      601 gcgagttgaa ccagtcgaga tccttgccct tgtcgagcaa gatctgcagc gcgcccaccc
      661 acacgaacaa caggatcagg ccgactcggt cgatgggcag cttcctggtc ggtgtttccc
      721 gggatttgag attgttccag cacagaagcg tacagaacac gcctacaggt acgttgatga
      781 ggaagatcca cggccacaca tagttgtcgg agatgtagcc gccgagcagc ggacccgcaa
      841 tgggcgccac cagcgtggtg agcgaccaga cggctagcgc ggcgttgcgc ttggaggggc
      901 cgaacacttc gatcagcagc gcctggctgc cggggatcat ggggccggac accgcaccct
      961 gcagcacgcg aaacgcgatc aaggacggga gactccaggc caatccgcac agcagcgacg
     1021 caatcgtgaa cagcgcgacc gaaatcacga aggtgcgcac gacgccgaag cgctgcatga
     1081 gccaccccgt gagcggcacc gatacgccgt ttgcgaccgc gaaggaagtg atcacccacg
     1141 tgccctgatc agcgctcact cccaagttgc cggcgatggt cggaatggac acgttcgcga
     1201 tggaggtgtc gagcacctgc atgaacgtgc ccaaggccag ggccgccgcg gttatggctt
     1261 tgcggccgcc ggtcagagca ggcgccgggg ccgcggcggc gtaatttgcg ctcacgagcc
     1321 ggcggccagc accgtcgagc cgctctcggt gcccaccttg tttttccggc tgagccgcgc
     1381 gggctcgtga gccttggggc ccgcgttgtc ggcgatgatc tccgcgatcc gcccatctac
     1441 cgccggatca tcgcccgcgc tcggttgcgt cggctgcggc tcattgcgca ccgccatggt
     1501 catgagagga cccgaggtgt catgcagatc cactcggacg ttggtcgaca ggccgacgcg
     1561 cagcggatgc gctttgagct cctggggatc gagaagaata cgtacgggga ggcgctgtac
     1621 gatcttgatc cagttccccg aggcgttctg cgaaggcagc agcgcgaaag cattgccgct
     1681 gcccgcggcg atgccgacca catggccgtg gtaggtcacg ccacgcccgt acaaatcggt
     1741 gcgcacggtg acgggctggc ctgcgcgcat gcgctcgagc tggccttcct tgaaattggc
     1801 gtcaatccac acatcatcga gcggcacgac cgccagcagc ggcgatcctg ccgccacgcg
     1861 ctgtccgacc tgcacggagc gcttggcgat caggccggtg accggcgagc tgagcgcggt
     1921 gcgatgcagg gcgagtgccg catttcgaac agcggccgcg gcggccagca cctgcgggtg
     1981 atccgcgatg gtggtaccgt cgatctgcgc taccgtctga ctcagttgct ggcgggcggc
     2041 ggcgacgttc gcacgggtgg tggtgaccgc gtcccgagcg tgggacagct cttcagcgga
     2101 aattgcgccg tcggcgatga gtccgccgcg ccgccgcagg tcttcgtccg cggttcgttc
     2161 cgcctgttcc cgctgatcga tctgggcgcg taattccttg ccttgcgcga acaggccacg
     2221 cacttgccgc acggcacggg ccagatccgc ttccgcattc gctgcggcga tctttgcatc
     2281 cgccgggtca agctgcagca agggctgccc ggcatgcacc gcctgcgtgt catccacatt
     2341 gagagcgatt accgtacccg gaatttcgct ggtcacctgc acgacgtcgc cgttgacgta
     2401 agcgtcgtcg gtggactcgt aatatcggcc gtcgaggaac cagtatccca agtagccgaa
     2461 gccgccgagc acgacgacgg cgcccagtcc gaacagtccc aggcggcggg aacggccggc
     2521 tggttggggg gaattgggtt cgcttggcaa ctcggattcg ctggtcactt tggatccacc
     2581 ggtgactgaa agttctggcg tgctcatgga tttgtccgcg taagggttcc tgaagaagtg
     2641 tctgtatgtg aggcatcggc gagcgcgccg gacgcacggg gatcgaaact gccgccgacc
     2701 gcgagcagca aggtcaccct cgatacgacc tggctggtca ggatgtccac catggaccgg
     2761 cgcgcggtga gcacctgcgt ttccgcgttg agaacggaca gatagctggc aagcccggcg
     2821 cgatagcgtt cctcggccag ccgataggcg gcctcggtgg cctcgagcgt ctgctgctga
     2881 tcgagccgct cgcggtccag tgcatccagg ttgctgattt ggtcgcccgt ctgctgcacg
     2941 gcgcgaagta cggtgtcgtt gtagcccgcg atcgctgcgt cgagctgcgc ctcgctgccc
     3001 tgatattccg cgcgcaggcg gcctccatcg aagagcggca gcgagatgag cggaccggca
     3061 ccgtaacccc gcgcgctcca ctgcaccaga ttactcatgc cgaatgcgcc gatgccggcg
     3121 agcgcgcgta aactcacatc cggatagaac gccgccttgg cggccagacg ctctgcgtcg
     3181 gccgcttcca cggtcaaccg tgacgacagt acgtcgggcc ggcgcgcgag cagattgatc
     3241 ggcagcgccc tcggcaccgg cagtgccgcc tccacgtgga ggaccgggcg ttggatcgac
     3301 gcataggact ccgccccctg cccggtcagc tgggcgagct gatggaccgc cagttccgca
     3361 gccgcctgcg cctgggcgcg cgccacccgt gcctgcggca attggccctc cgcctcacgc
     3421 aattcgagct gcgtatcgag ccccgcggca acacggcggc gagtgatgtc gacgatgttc
     3481 tgacgctgcg cctccgagcg ctcagcgatg tccaccagtg cgtattcgcg atagagctcg
     3541 acgtaggcct gggtgatggc gccggcgagc atgagccggg cgtagtcgac atcgaatcgt
     3601 gcggactgaa cgagattctg cgcgcgacgg acggcgtccg cctggcgtcc ccagaaatcg
     3661 agatcccacc ctaaactggc gccggcctgc cccatccaga agctgtgccc cgcgaggggc
     3721 gggggaataa cataattctg cggggcgcgc tggtacaggg cactggcgtc caggttgacg
     3781 ctcggccgca atccggcttg cgccgcttgt gcctgcgcga gcgctccgct cactcgggcc
     3841 tgggcctcgg tcagggttgg gctgtctttg agcccgaggc gaatcaggcg atcgagctgg
     3901 ggatcttgaa acgaatccca ccatccctct gcggccggct ccgc
//
LOCUS       DATGEM010000039         7025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_213111, whole genome shotgun sequence.
ACCESSION   DATGEM010000039 DATGEM010000000
VERSION     DATGEM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213111"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VKQ27_01005"
     CDS             <1..285
                     /locus_tag="VKQ27_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLY87536.1"
                     /translation="VDGMRSALLTALANWLDIWDRDGFAAVRSSWLARAHPPGTTLRV
                     VVGDRTIEGRFADLAPDGALLLDTAEGRKRYVAGDVSLSAAVGEEACPPA"
     gene            545..1144
                     /gene="phaR"
                     /locus_tag="VKQ27_01010"
     CDS             545..1144
                     /gene="phaR"
                     /locus_tag="VKQ27_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HLY87537.1"
                     /translation="MSGTVQAEPKTKPADTQPVVVKKYANRRLYNTESSSYITLDNLA
                     EMVRQGRDFVVYDAKTGEDITRSVLTQIIVEEEGKGRHLLPTAFLRQLIGFYGDQMQS
                     LVPRFLEQAMGAFAQQQEQVRAAMQKTMGSMGTLFPFGNMEEVSRQNMAMMERAFSLF
                     TPFYRGPDAGAPAQSEEIAALRAEVESLRRQLEEAHNKS"
     gene            complement(1150..2280)
                     /locus_tag="VKQ27_01015"
     CDS             complement(1150..2280)
                     /locus_tag="VKQ27_01015"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002295.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HLY87538.1"
                     /translation="MTRVKICGINDPVAFDTTIAAGADWLGFNFFPPSPRYVTPAQAA
                     QLSARSTGGAARVGLFVDPSEEDIAAALDVVPLDVLQLYGALDFAALHARFGLPVWRP
                     VGIETERDLPASAGGADRLLVEAKAPPGATRPGGNAVSFDWSLLRGWTAPSPWILAGG
                     LTAETVAEAVRVSGASAVDVSSGVERSRGVKDPALIRAFIANARAAGVVYRVATLADT
                     DALGVVHVQAWHEAYTGLIPDEVLAGLDPLRRAAMWHGLIGGRGGVQIAEQGGAIVGF
                     GSAGPQRDASLPFSAEIGALYVLRAAQRRGVGRRLMAMMARDLLDRGHASASLWVLEA
                     NAPARRFYEALGGRVITIREEERDGFSSVGAAYGWEDLRRLL"
     gene            complement(2277..2567)
                     /locus_tag="VKQ27_01020"
     CDS             complement(2277..2567)
                     /locus_tag="VKQ27_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HLY87539.1"
                     /translation="MRLLIGIPLLLVMVLFALSNRTPVTLVLWPTDFSLELPLSLAIL
                     AAMAIAFLVGGLLVWFSALAQRRRARRAEQEVRLLEAEVQSLKARLPSLPPA"
     gene            complement(2581..2880)
                     /locus_tag="VKQ27_01025"
     CDS             complement(2581..2880)
                     /locus_tag="VKQ27_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HLY87540.1"
                     /translation="MTKSELIAELAASNPHLRGADIELIVATIFDQITSALSQGERVE
                     LRGFGAFTVKRRNARTGRNPRTGEAVPVDEKAVPFFKAGKELRERVNKGRPARTG"
     gene            complement(2904..3752)
                     /gene="sppA"
                     /locus_tag="VKQ27_01030"
     CDS             complement(2904..3752)
                     /gene="sppA"
                     /locus_tag="VKQ27_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLY87541.1"
                     /translation="MLAVLAALLVTLRGAHVAFRGARVERVVVSGIITQDRKLNDAID
                     KLADNTDVKAVIVSVDSPGGSVAGGESLHDAIARVAAQKPVVTVMGGVAASAGYMISV
                     PAARIFASQATLTGSIGVLLETGDISGLLGKLGIASDPIVSGPLKDQPSFTKPLSPEG
                     RQVLQGLVMDMYDQFVSMVAQGRHMDPDKVRALADGRAYTGRQALNLGLVDQIGGEHD
                     ARAWLAKEKGVSASLPTEDLTTGGITSRVLSSSLGWIVDDLWKSVLSQGVTLDGAQAL
                     WQRSGD"
     gene            complement(3840..5591)
                     /gene="rpsA"
                     /locus_tag="VKQ27_01035"
     CDS             complement(3840..5591)
                     /gene="rpsA"
                     /locus_tag="VKQ27_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HLY87542.1"
                     /translation="MASSATVARTAPPPAPMQDHFKGENFASLLDETLGQDTGFDGSV
                     VSGRVLRLTDEFAIVDVGLKSEGRVALKEFGPPGTKPEVKPGDVVELYVERYEDKDGA
                     IILSREKARREEAWTNLEKAFETQQRVNGRIYGRVKGGFTVDLNGAVAFLPGSQVDIR
                     PVRDVGPLMESPQPFQILKMDRARGNIVVSRRAVLEETRAEQRTELIQGLKEGQILDG
                     VVKNITDYGAFVDLGGVDGLLHVTDIAWRRINHPSEALQIGQQVKVQVIRFNPDTQRI
                     SLGMKQLESDPWDGVSAKYPVSAKFTGRVTNITDYGAFVELEPGVEGLVHVSEMSWTK
                     KNVHPGKIVSTSQEVEVMVLDVDASKRRISLGLKQVQRNPWEQFVDEHAIGTEVEGEI
                     RNITEFGLFIGLSPDIDGMIHMSDISWDEPGELAIARYEKGQMVKAKVLDVDVEKERI
                     SLGIKQLKDDPAASVLATVNKGDVVTCVVTGIMPNGIEVKVNDVLTGFIRRAELARDK
                     ADQRPDRFAEGEKVDAKVVAVDRAARKLSLTIKGKEVEEEKQAMAEFGSSDSGASLGD
                     ILGAAIRRRNQQAQSEG"
     gene            5945..6607
                     /locus_tag="VKQ27_01040"
     CDS             5945..6607
                     /locus_tag="VKQ27_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HLY87543.1"
                     /translation="MTNPRLHPPAWADDPDVPDVPVPPGPEPREPDENKALGTPINEL
                     ESRLLRQRKVLVFGAIDDKVARDVTGRLLALAADAEPIDVYVNSPGGHVESGDTIHDM
                     IRFVDTIAPVRVIGTGWVASAGALIFLGAAKDRRFCLPNTRFLLHQPMGGVRGPATDI
                     NIEAIEIVKMRERINRIIARETGQPLEKVESDTDRNYWMSAEEAIAYGMVARIITTLK
                     DL"
     gene            complement(6627..>7025)
                     /locus_tag="VKQ27_01045"
     CDS             complement(6627..>7025)
                     /locus_tag="VKQ27_01045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLY87544.1"
                     /translation="QYVAPIQKAAWSRKTFEYGTWGGEFRSEFTPRPGEIVAQEHWCS
                     SGFANTDLDLQLKRHGIQQLIAIGLIAHTCVEATVRFAAELGYDVTVVKDATASYSER
                     EMHAALEVNLPNYARAIATASEVVASIASL"
BASE COUNT         1240 a         2554 c         2082 g         1149 t
ORIGIN      
        1 gtcgacggca tgcgcagcgc cctgctgacc gcccttgcca actggctgga catctgggac
       61 cgcgatggct tcgctgctgt ccgctccagc tggctcgcac gcgcccatcc ccctggcacg
      121 acgcttcgcg tagtcgtcgg cgaccgcacc atcgaaggcc gcttcgccga tctcgcgccc
      181 gacggcgctc tcctgttgga cacggccgag ggccgcaaac gctacgtcgc gggcgacgtg
      241 tccctctccg ccgctgtggg cgaagaggcc tgccccccgg cttgacccgg ggggtcaggt
      301 gaggcgggga accactccga caggtttcca cccgcgaaaa cagcccggtg cgtgctaaac
      361 cgcaaccatg cagtccacgc gctccgttat ccggtggatg gtgtcgccat tctgtgtcgc
      421 actcgtcctg tccgccgcgg gtgccgcagc accggatgtc cgcgccgcgc cgtgtcacgc
      481 agaaaaggct ttcgcaggtg cggcataagt tccatgctgc gcccaacgca gaacagaggc
      541 acgcatgtcc gggaccgtcc aggctgaacc caagaccaaa cccgccgaca cccagccggt
      601 ggtagtaaag aaatacgcca accgccggct gtacaatacg gaaagctcca gctacatcac
      661 gctggacaac ctggccgaga tggtccgcca aggccgtgac ttcgtggtct acgacgccaa
      721 gacaggcgag gacatcaccc gctccgtcct gacccagatc atcgtcgaag aggaaggcaa
      781 aggccgccac ctgctcccca ccgcgttcct ccgccagctc atcggcttct acggcgacca
      841 gatgcaaagc ctggtgccgc gcttcctgga gcaggccatg ggcgccttcg cccagcagca
      901 ggagcaggtg cgcgcggcca tgcagaagac catgggctca atgggcacgc ttttcccatt
      961 cggcaacatg gaggaggtca gccgccagaa catggcaatg atggagcgcg cgttcagcct
     1021 gttcacgcca ttctaccggg gccccgacgc tggtgccccc gcccagtccg aggaaatcgc
     1081 cgccttgcgt gccgaagtgg agtccctgcg ccgccagttg gaagaagcac acaataagtc
     1141 ctgatcagcc tacagcagcc gtctcaggtc ctcccacccg tacgcagccc caacagagct
     1201 gaacccgtcc cgctcttcct cacggatggt gatcacgcgc ccacccagag cctcgtagaa
     1261 ccgccgcgcc ggcgcgtttg cctccagcac ccagagcgac gccgatgcgt gtccccgatc
     1321 cagcagatcg cgcgccatca tcgccatcag ccgtcgccca actccccgcc gctgcgccgc
     1381 ccgcaacaca tagagcgcgc caatctccgc ggaaaacggc aaggaggcgt cccgctgcgg
     1441 ccccgccgaa ccgaagccca cgatcgcccc gccctgttct gcaatctgca cgcccccccg
     1501 accgccgatg agcccgtgcc acatcgccgc ccgccggagc gggtcgagcc cagccagcac
     1561 ctcatcgggg atcaggccag tatatgcctc gtgccacgct tggacatgga ccacgccaag
     1621 cgcatcggtg tcagcaagcg tggcaacccg atagacgacc cccgccgccc gcgcattcgc
     1681 gatgaaggcc ctgatcagcg ccggatcctt gaccccccgg ctccgctcta ccccggaaga
     1741 aacgtcgact gccgatgcgc cgctcacacg caccgcctcc gccacagtct ccgccgtcaa
     1801 tccgcccgcc aggatccagg gcgaaggggc tgtccagccg cgcagcaggg accagtcgaa
     1861 gctgaccgcg ttgcccccgg gccgggttgc tcctggcgga gccttcgcct cgaccagcag
     1921 ccggtccgca ccacctgccg aagccggcag gtctcgctcc gtctcaatcc cgaccgggcg
     1981 ccacaccggc agcccaaagc gcgcatgcag cgcggcgaag tccagcgccc cgtagagttg
     2041 cagcacgtcc agcgggacga cgtcgagcgc cgcggcgata tcctcctcag acgggtcgac
     2101 gaacagcccg acccgcgccg ccccgcctgt tgaccgcgct gacaactgag ccgcctgcgc
     2161 cggcgtcaca taccgcggcg atggcgggaa gaaattgaac cccagccagt cagcccccgc
     2221 agcgatggtc gtatcgaacg ccaccggatc attgatcccg cagatcttga cccgggtcat
     2281 gccggcggca gcgagggcag gcgcgccttc agtgactgca cctccgcctc cagcagccgc
     2341 acctcctgct cggcccgccg cgccctgcgg cgctgcgcga gggcggagaa ccagaccagc
     2401 agcccgccca ccaggaatgc gatcgccatc gcagccagaa tggccagcga tagcggcagc
     2461 tccaaagaaa agtccgtcgg ccagagcaca agcgtcaccg gtgtccgatt ggacagcgcg
     2521 aacagcacca tcaccagcag cagcggaatt ccaatcagca ggcgcattgc ggcgcacccc
     2581 tcaacccgta cgcgccggcc gtcccttgtt gacccgctcg cgcaattcct tgcctgcctt
     2641 gaagaatggc acggccttct cgtccacggg caccgcctct cccgtgcgag gattccgccc
     2701 cgtgcgcgca ttccgccgct tcacggtgaa cgcgccaaat ccgcgcaact cgacccgctc
     2761 gccctgcgac aacgcactgg tgatctggtc gaatattgtt gccacgatca actcgatgtc
     2821 cgcgccccgc aggtgcgggt tgctggcggc cagttccgct atgagttccg atttggtcat
     2881 gcacccggcc agcgacggac aattcagtcg ccggaacgtt gccagagcgc ctgcgccccg
     2941 tcaagcgtaa ctccttggga caaaacactt ttccaaaggt catcaacaat ccacccgagg
     3001 ctcgacgaca acacccggga cgtaatgccg ccggttgtga ggtcctcggt gggcaaactg
     3061 gcggacacgc ccttctcctt ggcaagccac gcgcgtgcgt cgtgctcacc gccgatctgg
     3121 tcgaccagcc cgagattcag cgcctggcgc ccggtatatg cccggccatc ggccagcgcc
     3181 cgcaccttgt ccgggtccat atggcgcccc tgggcgacca tgctgacgaa ctggtcgtac
     3241 atatccatga ccaatccctg cagcacctgc cgcccctccg gcgacagcgg cttggtgaag
     3301 ctcggctggt ccttcagcgg ccccgagact atcggatcgg acgcgatccc gagcttgccc
     3361 agcaacccgg agatatcgcc ggtttccagc agcaccccga tggacccggt cagcgtcgcc
     3421 tggctcgcga agatgcgcgc ggcgggaacc gagatcatat agcccgccga agccgccacc
     3481 ccgcccatca ccgtgaccac cggcttctgg gcggccaccc gcgcaatcgc gtcatgcagc
     3541 gactcgccgc ccgccacgct gccgccgggg ctgtcgaccg agacgatcac cgccttgacg
     3601 tccgtattgt cggccagctt gtcgatcgca tcgttgagct tgcggtcctg ggtgatgatc
     3661 ccgctcacca ccacccgctc gacccgtgcc ccccggaacg cgacatgtgc gccacgcagc
     3721 gtcaccagca gcgcggccag caccgccagc acagcaaagc tccgccagaa aaacaggcgc
     3781 cgcttgaggc ggcgcctgtc caacagaaga tcggtctcga ggctcacagc cggctgacct
     3841 tacccctccg actgggcctg ctggttgcgg cggcggattg cggcgccgag gatatcgccc
     3901 agcgacgcac ccgaatcgga cgaaccgaac tcggccatcg cctgcttctc ttcctcgacc
     3961 tccttgccct tgatggtcag gctgagcttg cgcgccgccc ggtccactgc aaccaccttg
     4021 gcatccacct tctcgccctc ggcgaagcga tccggacgct ggtccgcctt gtcgcgcgcc
     4081 agttcggccc gccggatgaa gccggtgagc acatcgttga ccttcacctc gatgccgttc
     4141 ggcatgatgc cggtcacgac gcacgtcacc acgtcgccct tgttcaccgt cgccagcacg
     4201 gacgccgccg gatcgtcctt gagctgcttg atgccgaggc tgatccgctc cttctcgaca
     4261 tcgacgtcga gcaccttggc cttcaccatc tggccttttt cgtagcgcgc gatggcaagc
     4321 tcgcccggct catcccagga gatgtcggac atgtggatca tgccgtcgat atccggcgac
     4381 aggccaatga acagcccgaa ctccgtgata ttgcggattt ctccctcgac ctcggtcccg
     4441 atcgcatgct cgtcgacgaa ctgctcccag ggattccgct gcacctgctt caggccgagc
     4501 gagatgcggc gcttcgaggc gtccacatcc agcaccatca cctccacctc ctggctggtg
     4561 gagacgatct tgccgggatg cacgttcttt ttcgtccacg acatctcgct gacatgcacc
     4621 aggccttcga cgcccggctc aagttccacg aacgcgccgt agtcggtgat gttggtcacg
     4681 cgcccggtga acttcgcgct caccggatac ttggcggaca cgccatccca cggatccgat
     4741 tcaagctgct tcatgcccag gctgatgcgc tgcgtgtccg gattgaaccg gatcacctgc
     4801 accttgacct gctggccaat ctgcaacgcc tcggacggat ggttgatccg ccgccatgcg
     4861 atatcggtca cgtgcagcag cccgtccacg ccgcccagat ccacgaaggc gccgtagtcg
     4921 gtgatgttct tcactacgcc gtccaggatc tggccttcct tcagcccctg gatcagctcg
     4981 gtgcgctgct cggcgcgggt ctcttccagc accgcgcggc gcgataccac gatattgccg
     5041 cgcgcccggt ccatcttcag gatctggaac ggctgcggcg actccatcag cggcccgaca
     5101 tcgcggaccg ggcggatatc gacctggctg cccggcagga atgccacggc gccgttcaga
     5161 tcgacggtga agccgccttt cacccggcca tagatccggc cgttgacccg ctgctgggtc
     5221 tcgaacgcct tctcgaggtt ggtccacgcc tcctcacgcc gcgccttctc gcgcgacagg
     5281 atgatggcgc cgtccttgtc ctcgtagcgc tcgacataca gctcgacgac atcgccgggc
     5341 tttacctcgg gcttggtgcc cggtggaccg aattccttca gcgcgacgcg gccttcggac
     5401 ttcaggccta catcgacgat ggcgaattca tcggtgagcc ggaggacccg accagacacg
     5461 accgacccgt cgaagccggt atcctggcca agggtctcat cgaggaggga ggcgaaattc
     5521 tcgcctttga agtggtcctg catcggggca ggtgggggag cggtgcgggc aacagtggca
     5581 gatgaagcca tgtggtggtt gttttccgtt acgggtggtc cgggcgggac gcccggggtt
     5641 gaccgttggt gtgcgcgttc cacgcaggaa acacgtcttg cccgcgggtt gtggccgcgg
     5701 gccgggttgc aattacgtca agtcgccgga gagatacgct ccattccgct caagtcaagc
     5761 caggagttgg cttcctcccc gcgatgcgct gccgttcact aaccagacag caatgatcgt
     5821 ggctgcaatt gcagggagtg attatagctg ccttaggctg ttgcggatca actcggcggc
     5881 gttcggccaa ctggacctgc gcccgccccg cacctagatt ccgctcctga cccggagaca
     5941 caccatgacc aatccccgcc tgcacccccc tgcctgggcc gatgatccgg acgtccccga
     6001 cgtccccgtc ccgcccggcc cggaaccgcg ggagccggac gagaacaagg cactcggaac
     6061 gccgatcaac gagctggaaa gccgtctgct gcgccagcgc aaggtgctgg tcttcggtgc
     6121 catcgacgac aaggtggcgc gcgatgtcac cggccgcctg ctggcgctcg cggccgacgc
     6181 cgaaccgatc gacgtctacg tcaattcgcc cggcgggcac gtcgagagcg gcgacacgat
     6241 ccacgacatg atccgcttcg tcgataccat cgcaccggtg cgggtgatcg gtaccggctg
     6301 ggtcgccagc gccggcgcgc tgatcttcct tggcgccgcc aaggaccgcc gcttctgcct
     6361 gccgaatacc cgcttcctgc tgcatcagcc gatgggcggg gtgcgcggcc cggccaccga
     6421 catcaacatc gaggcgatcg agatcgtcaa gatgcgcgag cgcatcaacc gcatcatcgc
     6481 ccgcgaaacc ggccagccgc tggaaaaggt cgagagcgac accgaccgca attattggat
     6541 gagcgcggag gaggccatcg cctacggcat ggtcgcgcgg atcatcacca ccctgaagga
     6601 cctgtagcct cgggccagcc ggccggtcag agcgaagcga tcgaagccac gacttcactc
     6661 gcggtggcga tcgcacgggc gtagttcggg agattgacct cgagcgccgc gtgcatctcc
     6721 cgctctgagt agctcgcggt ggcgtccttc acgaccgtga catcgtaacc aagttcagcg
     6781 gcgaagcgta ccgttgcctc gacgcacgtg tgcgcgatga gcccgatggc gatgagctgt
     6841 tggatgccgt gccgcttcaa ctgcagatcg agatcggtgt tggcgaagcc actggagcac
     6901 cagtgctcct gagccacgat ctcgcctggc cgcggagtga actcgctgcg gaactcgcca
     6961 ccccaggtcc cgtattcgaa ggtcttgcgc gaccaggccg ccttctggat cggggcgaca
     7021 tactg
//
LOCUS       DATGEM010000040         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_784753, whole genome shotgun sequence.
ACCESSION   DATGEM010000040 DATGEM010000000
VERSION     DATGEM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784753"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VKQ27_01050"
     CDS             complement(<1..555)
                     /locus_tag="VKQ27_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLY87545.1"
                     /translation="MATVLLIDVGAVMSLTNSKWQDQALLFQLRTSFRIALSRVSERT
                     ERSRTMSLSIEGIDVPDSKLAHEITELVRDTETPLLFHHSRRVYCWGALTGRRSRLSF
                     DAELLYAGAMFHDMGLTSAHSSASERFEVDGANAARAFLRSHGIAQQNIDLVWTAIAL
                     HTTPGIPQHMHPIVALVTAGVEMDV"
     gene            536..1498
                     /locus_tag="VKQ27_01055"
     CDS             536..1498
                     /locus_tag="VKQ27_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLY87546.1"
                     /translation="MSRTVAILAMPGVQLLDVSGPLDVFAEANVQASRNLYRLQVIGG
                     ASSEIRSSSGTRLVADRCVGEPAGEAIDTLLVAGCPNLGAARSPAAVIAWLREVAPRT
                     RRFGSVCTGAFLLAEAGLLDGKRITTHWAVADRLAAAYPAINVDADAIHVCDGKLRTA
                     AGVTAGLDLALALIEEDLGRDIAMRVARQLVMFFKRPGGQMQFSARGEPVPAARSVLQ
                     EILRFVAANPGADHSVGSLALRANLSPRHFARLFHGEIGVTPAAWIETARVSAARRLL
                     EMGREAPKQVAVRCGFGNADVLRRAFARHVGITPAEYRRRFATG"
     gene            1558..>2250
                     /locus_tag="VKQ27_01060"
     CDS             1558..>2250
                     /locus_tag="VKQ27_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87547.1"
                     /translation="MILTASSDIARQTRRKTANGWHTTFIGENRNTLKEGQAAPEPGV
                     LYPMAFLVEKEPHAVVKPHFHQADQYQVVVQGGGRLGTHDVGTVAVHYTDAWSAYGPI
                     VAADEGIAWFTLRNAWDSGARYMPAARDQLRAARQRNFAHREATAPAMPVLPTGELAR
                     AGQIECMAALEQTADGMASWRYRLPGCASLAGPAPGDGGGQFWIVLSGSAAAGGAADL
                     PANSCLFVGPDDG"
BASE COUNT          380 a          662 c          798 g          410 t
ORIGIN      
        1 gacatccatc tccacgcctg ccgtcacgag cgcaacgatt ggatgcatgt gctgcgggat
       61 gccgggcgtc gtatgcagag caatcgccgt ccatacgagg tcgatattct gctgcgcaat
      121 cccgtgactg cgcagaaacg cgcgggcagc gttcgcgcca tcgacctcga agcgttcgct
      181 ggcgctgctg tgcgccgatg tcagtcccat gtcatggaac atcgcaccag catagagaag
      241 ctcggcgtcg aacgagaggc ggctcctgcg gccggtcagt gcgccccagc aatagacgcg
      301 tcgcgaatgg tgaaacagca gcggcgtttc agtgtctctg accaactccg tgatttcatg
      361 cgcgagtttg ctgtcaggaa cgtcgattcc ttctatcgat agcgacatcg ttctgctcct
      421 ctcggtgcgt tctgatacgc gcgataaggc gatccgaaac gatgttctca attgaaataa
      481 aagcgcctga tcctgccatt tcgagttggt gagagacatg accgcaccga catcgatgag
      541 caggacagtc gccattctgg caatgccggg cgtccagttg ctggacgtgt ccggaccgct
      601 tgacgtgttc gccgaagcga atgtgcaggc gagcaggaac ctttatcgtc tgcaggtgat
      661 tggcggtgca tcaagcgaaa tacggagttc gtctggaacg cggcttgttg ctgaccgctg
      721 cgtgggagag ccagcgggcg aggcgatcga cacgctgctt gttgcaggat gcccgaacct
      781 tggggcggca cgctcgccgg ctgccgtgat tgcgtggctg cgagaggttg cgccgcggac
      841 gcggcgcttt ggctcagtgt gcaccggagc tttcctgctc gccgaggccg ggctgctgga
      901 tggcaagcgg atcaccaccc attgggcggt ggccgatcgg ctcgccgcgg cctatccggc
      961 gatcaacgtg gatgccgatg cgatccatgt gtgtgacgga aagctgcgca ccgccgcggg
     1021 cgtcacggcg ggcctcgatc tcgcactggc gctgatcgag gaggatctgg ggcgcgacat
     1081 cgctatgcgc gtcgcgcggc aactcgtgat gttcttcaag cgcccgggtg gtcagatgca
     1141 gttcagcgcc aggggagagc cggtgccggc agcacggtcg gtcctgcagg agatcctgcg
     1201 ctttgtcgcg gccaatccgg gagcggacca ctccgtcggc agtcttgcct tgcgagcgaa
     1261 cctcagccca cgacatttcg cgcggctgtt ccatggcgag atcggggtca cgcccgcggc
     1321 ctggattgaa acggcgcggg tgtcggcggc gcgacggctg ctggagatgg ggcgcgaagc
     1381 gccgaaacag gtggccgttc ggtgtggctt cggcaacgcc gacgtgctgc gccgggcttt
     1441 cgctcgccat gtcggcatta cgccggccga gtatcgcagg cggttcgcga ctggctgagg
     1501 cggcggcttg gcgcgatagc ggcgggcagc gtagactcgg cagcggagga actttccatg
     1561 atcctgaccg caagcagcga catcgcccgc cagacgcgcc gcaagaccgc caatggatgg
     1621 cacaccacct tcatcggcga gaaccggaat acgctgaagg aagggcaggc tgcacccgag
     1681 cccggcgtcc tgtatccgat ggcgttcctg gtggagaagg agccgcatgc ggtggtgaag
     1741 ccgcatttcc atcaggctga tcagtatcag gtggtggtac agggcggcgg ccggctcggc
     1801 acccacgacg tgggtacggt ggcagtgcac tatacggatg cgtggtcggc gtacgggccg
     1861 atcgtcgcgg cggacgaagg gatcgcctgg ttcactttgc ggaacgcctg ggattctggg
     1921 gcgcggtata tgccggcagc gcgggaccag ttgcgcgcag cccggcagag gaattttgcc
     1981 catagggagg cgacagctcc ggcgatgcca gtcctgccga caggcgaact ggctcgcgca
     2041 gggcagatcg agtgcatggc ggctctcgaa cagacggcgg atggcatggc ttcctggcgg
     2101 tatcggctgc ccgggtgcgc cagcctggcg gggccggccc cgggtgacgg cggcggccag
     2161 ttctggatcg tgctgtccgg ttcggcagcg gctggcggtg cagcagattt gccggcgaat
     2221 tcgtgcctgt tcgtcggacc ggatgacggg
//
LOCUS       DATGEM010000041         6028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_212768, whole genome shotgun sequence.
ACCESSION   DATGEM010000041 DATGEM010000000
VERSION     DATGEM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212768"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VKQ27_01065"
     CDS             <1..248
                     /locus_tag="VKQ27_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87548.1"
                     /translation="VLGLGGACRAENAHLGGPPLLTAQTSGTTPFRLSSHVGDVGHML
                     IVGGAVDRARWSDPLCADRRRPTPLQRPPSKGYEEEP"
     gene            complement(394..1644)
                     /locus_tag="VKQ27_01070"
     CDS             complement(394..1644)
                     /locus_tag="VKQ27_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HLY87549.1"
                     /translation="MGPLFRRVGQALRRDGYGVYKINFNGGDRLFWRLPNGIDYRGSV
                     EEWPRALQQIVGDLKITDVVLFGDCRPMHMAAIAVCKAIQIPVHVFEEGYIRPDWVTL
                     ELGGVNGHSSLPRDPAWYRAEAAKLGPAPEHHAVPSSFRRRALEGVAYNAADVLTRWH
                     YPFWENHRPWHPLVEGMGWLRRLGRRKAAAARSETLIAALDAERPPYILFPLQLDSDA
                     QIRLHSSFAGIADALRTVIQSFAAHARPSLRLVVKEHPLDNGVRDWRGETAALAALYG
                     IADRVDYLESGDIVPVARNAQGVVTINSTSGTLALALGVPVIALGQAVYDIEGITFQG
                     SLDDFWHAPGKPDCETFAAFRRVLIDRCLVAGGFFSEEALVKVTDGVIRRLKEASPLV
                     QRVLAHSEDNSRNVPQQRLVPDQI"
     gene            1977..3494
                     /locus_tag="VKQ27_01075"
     CDS             1977..3494
                     /locus_tag="VKQ27_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLY87550.1"
                     /translation="MTNSVISFLAMLSGSLLLDRTVRPRAGLNWSWQGLLLHLLVLTA
                     TFGLVLAGSGSVVVAAVLAIALMALFSTASNAKHAMLGEPILFSDLALFAGLFRHPRF
                     YWTAISVWQRWLLAIGAVALVSALALLFVPRLVPHLTGLGLLLFATGTLFLLLDSRSF
                     RALAPAPDVDADLMRHGLIATLMLYWLRWRYTPDPAACPALEEEDKGDAWPDLVVIVQ
                     CESFADPVELTGNPEHALPGLTRARTSAWQWGDLGVSGFGAYTMRTEYGVLFGRSEAA
                     LGFRRYDPFLTAHGETSYALSARLSGRGYHCSFVHPHDMRFYGRDQLMPATGFDRLIG
                     EESFPPVSPDSGRYVDDRTLGASMGELIDRAVERTFLYAVTMENHGPWDKDRLTGSSG
                     GLDAYLRHLRNSDTMLTDLIERLSGAGQSALLVFFGDHRPSIPGVTEPGGDRHTPYVM
                     LRFASDGRILTGANRRADLAPDALHHAILRCVQGRDRDGMMPGVAAAVSPPAASG"
     gene            complement(3469..4260)
                     /locus_tag="VKQ27_01080"
     CDS             complement(3469..4260)
                     /locus_tag="VKQ27_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87551.1"
                     /translation="MKSNNSQAGVPTRILVTGASGGLGEAIAHHYAAPGRTLLLWGRN
                     QERLVEVAEACRSAGAQAITRSLDLTDSAAAIAALEEEDDSAPIGLAVFAAGLGDIRP
                     PDRLVEDAAQIIRLGLVNFVAPSAMAATLASRMAIRRGGRIALIGSAAAFHALPFAAA
                     YAGSKAGLARFAEALRLGVAVHGVSVTLVSPGFIDTAAGRKVSGPKPFLMQPSSVAAR
                     IAKATERGQAHLVLPWPFALLRVVERLLPGLLRDRLLLSLTPPAD"
     gene            4384..4995
                     /locus_tag="VKQ27_01085"
     CDS             4384..4995
                     /locus_tag="VKQ27_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLY87552.1"
                     /translation="MIKDRSRPQRLRLFRDERMEQLTLISPLGFATTWLVVLMLALVA
                     SWGRASVTVSLGLVLLGILIWTLFEYAMHRFLFHLTPKSQLGQWLIFLMHGNHHVDPG
                     DRYRNIMPPIVSLWVIGGFWGVSVLLLGPVGSVLSLGFAIGYVVYDGIHYACHQFPMR
                     SPVLRQLKRHHIRHHYAKQEGNYAITAIFWDRVFRTDVRVKGR"
     gene            complement(5018..>6028)
                     /locus_tag="VKQ27_01090"
     CDS             complement(5018..>6028)
                     /locus_tag="VKQ27_01090"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLY87553.1"
                     /translation="KALRFLDAMEGRWTSEGGNVEQRSLTEVLPSLLGLLPERPRGGG
                     GVYVQASPHHLTRPTLVRRILEREGARFLCLVHDLIPIEYPEYARPNGAALHRKRIET
                     VTALANGVIVNSTATGESLKPWLEKAGRKVDMRVALLGTHEIPLPTAPKEEPARPYFV
                     CIGTIEARKNHLLLLNLWRHMAETQAPDTIPHLIIVGRRGWENEQVVDMLERCAALQG
                     HVEELNGCSDQRMHELLRGARALLLPSFAEGYGMPVAEALSVGTPVLCSDLPALREAG
                     GVVPDFLDPLDGPGWKAHILDYCQAGPRYAAQLQRLPYWHRPSWADHIRIVCEAIDDL
                     QR"
BASE COUNT         1024 a         1892 c         1915 g         1197 t
ORIGIN      
        1 ttgtcctcgg tctgggcggg gcctgccgtg ccgagaatgc gcatctgggc ggaccgccgc
       61 tgctgacggc gcagaccagc ggcacgacgc cgtttcgtct gtcgagccat gtcggcgatg
      121 tcggtcatat gctgatcgtc gggggagcgg tcgatcgtgc ccgatggtcg gatccattat
      181 gtgccgatcg acggcggcct acgccccttc agcggcctcc ctcgaagggt tacgaagagg
      241 agccgtaaga gtgatccgat agacgaccgt ctatagcgtt ttgtatctct gtggtaaccg
      301 ccggcaggtc cattaaagct tcacaattct ctgccagccg ctcagcggag gccggaggtc
      361 acattcgaat tgttacgtct acccattcga acctcaaatt tgatcgggta caaggcgttg
      421 ttgagggaca ttgcgactgt tatcctcgct gtgcgccaaa accctctgga ccaatgggct
      481 agcctctttc aggcgccgga taacgccgtc cgtcaccttg accaacgctt cttccgagaa
      541 gaatccgcca gccaccaggc accgatcgat gagtacgcgc cggaaggcgg cgaaggtttc
      601 gcagtccggc tttcctggcg cgtgccagaa gtcgtccaga gatccctgga aggtaatacc
      661 ctcgatgtca tatacggcct gacccagcgc gatcaccggc acgccaagag ccagcgccaa
      721 cgtgccgctc gtgctgttga tggtcaccac gccctgtgca tttcgggcga ctggaacaat
      781 atcgccgctt tccagatagt ccacacggtc agctataccg tagagggccg cgagagccgc
      841 ggtttcaccg cgccaatcgc gtacgccgtt gtctagcgga tgttccttga ccaccagccg
      901 tagcgaggga cgtgcatggg cagcgaagga ttggatgacg gtccgcaacg cgtcggcgat
      961 accagcaaag gaggagtgca ggcggatctg cgcgtcggaa tcgagttgta gcggaaagag
     1021 tatataagga ggacgctcgg catccaaagc ggcaatcaat gtttcggatc gcgccgcggc
     1081 agccttgcgt cggcccagcc tcctcaacca gcccatacct tccaccagcg gatgccaagg
     1141 gcggtgattt tcccaaaagg ggtaatgcca tcgggttagg acatccgcgg cgttataggc
     1201 caccccctcc agcgcgcgcc gtcggaagga cgaggggaca gcatgatgct ccggcgcggg
     1261 accaagcttg gccgcttccg cgcgatacca ggcgggatct cgcggaaggc tcgaatggcc
     1321 gttcacgcct cccagttcga gcgtcaccca gtccggccgg atatagcctt cttcgaagac
     1381 gtgaacgggg atctgtatgg ccttgcacac cgcgattgcc gccatgtgca tggggcgaca
     1441 atcgccgaac aggaccacat cagtgatctt gaggtcacca acgatttgct gcagcgccct
     1501 gggccactcc tccactgaac ctcggtaatc gattccattg ggcaggcgcc agaatagccg
     1561 gtcgccgcca ttgaaattga tcttgtagac tccgtagccg tcacgtcgaa gcgcttggcc
     1621 gacccggcgg aagaggggcc ccatcagtcc ctgcagcagc aagatattgc gctgcgcgag
     1681 tggctgcgcc gcacctggag ccgcaacctg gggcgcgggc accgaagttt cggcgaacga
     1741 attgggcatc cgactttcct tcgtcgacat gttacgcaca gggcaacggc gggtggaaac
     1801 gttccgctac gccgtcaccg gatgaacact taggccgcta gatatattcc gcgaccggag
     1861 caagcttgtc cggcggtgac agcttcccgc ctcctcgcta tagccgcgcc atcccttacc
     1921 cttcggcaca ttgatgccgg agtgacctta atccaggctg acaacgcggc ggaactttga
     1981 cgaacagcgt aatttccttc ctcgcgatgc tgtctggctc gctgttgctg gaccggaccg
     2041 ttcggccgcg cgctggactg aattggtcct ggcaggggct tctgctccat ctgctggttc
     2101 tgacagcgac gtttggactc gttctcgccg gttctggaag cgtggtggtc gccgcggtgc
     2161 tggcaattgc gctgatggca ctgttttcca ccgcctccaa tgccaagcat gccatgctgg
     2221 gcgaaccgat ccttttctcg gaccttgcgc tgttcgcggg gcttttccgc catcctcgtt
     2281 tctattggac ggcgatctcg gtgtggcagc gctggctgct ggcgatcggg gcggtggccc
     2341 tggtatcggc attggctctg ttgttcgtgc cccgcctcgt gccgcatctc acggggctgg
     2401 ggctgttgct gttcgcgacg gggacattgt tccttttgct cgacagtcgg tcattcaggg
     2461 cgctggcgcc agcgccggat gtcgatgcgg acctcatgcg tcacggcctg atcgcgacgc
     2521 tcatgctcta ttggcttcgc tggcgctaca cgcccgatcc cgccgcctgt cccgcgctgg
     2581 aggaagagga taagggcgat gcgtggcccg atcttgtcgt gatcgtgcag tgcgaatcct
     2641 tcgccgatcc tgtcgagctc acgggcaatc ccgagcatgc tctgcccgga ctgacgcgcg
     2701 cgcgcacgtc agcctggcag tggggtgatc tcggcgtcag cggctttggc gcctatacga
     2761 tgcgcaccga atatggcgtg ctgttcgggc gcagcgaagc ggctcttggc tttcgccgct
     2821 atgatccgtt cctgactgca catggcgaga cctcctatgc cttgtcggcc aggctgagcg
     2881 ggcgcggtta tcactgttcg ttcgtccatc cgcacgacat gcgcttctac gggcgggacc
     2941 agctgatgcc ggcgaccggc ttcgatcgct tgatcgggga ggagagcttc ccgccggtat
     3001 ctccggacag cgggcgctat gtcgatgaca ggacgctcgg cgcatccatg ggcgaactca
     3061 tcgacagggc tgtcgagcgg accttcctct atgccgtgac catggaaaat cacggcccct
     3121 gggacaagga tcgtctcacc ggttcgtccg gtgggctcga cgcctatctg cgccatttgc
     3181 gcaacagcga taccatgctg acggatttga tcgagcggct ttccggtgcc ggccaatctg
     3241 cgctgctcgt ctttttcggg gatcaccggc cgagcattcc gggcgtgaca gagcccggcg
     3301 gtgatcgtca cacgccctat gtgatgctgc gcttcgcgtc tgatggacgc atcctgacgg
     3361 gcgccaatcg gcgggcggat cttgcccccg atgcactcca ccatgccatt ttgcggtgcg
     3421 tgcaaggccg cgatcgggac gggatgatgc cgggcgtggc agccgccgtc agtccgccgg
     3481 cggcgtcagg ctgagcagca gccggtcgcg cagcaggccg ggtagcaggc gctccaccac
     3541 cctcagcagc gcgaagggcc atggcaggac gagatgggcc tgcccgcgtt cggtggcttt
     3601 ggcgatgcgc gccgcgacag aggacggctg catcaggaag ggcttgggcc ccgacacttt
     3661 ccgcccggcg gcggtgtcga tgaagccggg cgagacgagc gtgacggaga ctccatgcac
     3721 ggccacgccg aggcgcaagg cttctgcgaa ccgggcgagg cctgccttcg atcccgcata
     3781 ggcagccgcg aagggtaggg catggaaggc cgctgccgat ccgatcagcg cgattcgtcc
     3841 cccgcgtcgg atggccatgc ggctggcgag cgtcgctgcc atggcgctgg gggcgacgaa
     3901 attgaccagc cccagccgaa tgatttgcgc ggcgtcctcg accagcctgt cgggaggccg
     3961 gatgtcgccc aggccagcgg cgaacaccgc caggccgatc ggcgctgaat catcctcttc
     4021 ctcgagtgcg gcgatcgccg cggcactgtc ggtgaggtcg agcgagcgag tgatcgcttg
     4081 tgcacccgcg ctccggcagg cttccgcgac ctcgaccagt cgttcctggt tgcggcccca
     4141 gagcaacaat gtcctgcctg gcgctgcgta atgatgagcg atcgcctcgc ccagaccgcc
     4201 cgacgcaccg gtgacaagga ttcgcgtggg tactcctgcc tggctattgt tacttttcaa
     4261 cacttcgaat ccactttgtt tggtcacggc aattcgcgac aatgcggctt gacagggtgc
     4321 accccgctct ggccatagcg gagtcatgag tatgctttcg cgccgtgggg acgggaacgg
     4381 ttgatgatca aggaccggtc acgtccgcaa cgattaaggc tttttcgcga cgagcgcatg
     4441 gagcagctta cgctgatttc tccacttggc ttcgcgacga cctggcttgt ggtgctcatg
     4501 ctcgcgctgg tcgcgagctg gggaagagcg agtgtcactg tctctctggg gctcgtcctg
     4561 ctcggcatcc tgatctggac gctgttcgaa tatgcgatgc accgcttcct cttccacctg
     4621 acgccgaagt cgcagctggg acagtggctg attttcctga tgcatggaaa ccaccatgtc
     4681 gatccgggcg atcgttatcg caacatcatg ccgccgatcg tcagtctctg ggtgattggc
     4741 gggttctggg gtgtttccgt gctgctgctc gggcccgtgg gatcggtgct ctctctaggc
     4801 ttcgctatcg gttatgtggt ctatgacggc atccattatg cctgccatca attcccgatg
     4861 cgcagtcccg tgctgcggca attgaagcgc caccatatcc gccatcacta tgccaagcag
     4921 gaaggcaatt acgcgatcac cgcgatcttc tgggatcgtg tgttccgtac cgacgttcgc
     4981 gtcaaagggc gctgatcttc gtatcgacct gcggtgatca ccgctgcagg tcgtcaatcg
     5041 cctcgcacac aatccggata tggtcggccc aggacggccg atgccaatat ggcaggcgtt
     5101 gcagctgcgc ggcgtagcgt ggaccggcct ggcaatagtc caggatatgc gccttccatc
     5161 ccggcccatc gagcggatcg aggaagtccg gcaccactcc gccggcttcg cgcagcgcag
     5221 gcaggtcgct gcagagaacc ggcgtcccca ccgacagcgc ctcggcgacc ggcatgccat
     5281 agccctcggc gaaggacggt agcagcagcg cgcgcgcgcc acggagcagt tcgtgcatcc
     5341 gttgatccga gcagccgttc agctcttcga catggccctg cagtgcggcg cagcgctcca
     5401 gcatgtcgac gacctgctca ttctcccagc cgcgacggcc cacgatgatc aggtggggaa
     5461 tggtgtccgg agcctgcgtt tccgccatgt ggcgccacag gttgagcagc agcaggtggt
     5521 tcttgcgagc ctcgatcgtg ccgatgcaga cgaaataggg tctggccggc tcctctttcg
     5581 gcgccgtcgg caggggaatc tcgtgcgtgc cgagcagggc tacgcgcatg tcgaccttgc
     5641 ggccagcctt ctccagccag ggcttgagcg attcccccgt cgcagtggag ttgacgatga
     5701 cgccattggc cagagcggta acggtttcga tccgcttgcg gtggagtgcg gcgccgttgg
     5761 gacgcgcata ttcgggatat tcgatcggga tcagatcgtg caccaggcat aggaaccggg
     5821 cgccttcgcg ctccaggatg cgccgcacga gtgtcggcct ggtcagatga tggggcgagg
     5881 cctgcacata gacgccgcct ccgccacgcg gccgttccgg caggagccca agcagcgacg
     5941 gcagcacctc ggtaagcgag cgttgctcga cattgccgcc ctcactcgtc cagcgcccct
     6001 ccatcgcgtc gagaaagcgc agggcctt
//
LOCUS       DATGEM010000042         9467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_669125, whole genome shotgun sequence.
ACCESSION   DATGEM010000042 DATGEM010000000
VERSION     DATGEM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669125"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1176
                     /locus_tag="VKQ27_01095"
     CDS             145..1176
                     /locus_tag="VKQ27_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLY87554.1"
                     /translation="MGQYVGLDVSMKETAISIRQDGKRIWRGKCASDPQLLATVIRKR
                     APQAQRVVFETGPLSVWFYHALVAEGLPAICIDARHAKAALDMAANKTDANDADGLAQ
                     LAEVGFYREVRVKGFDSMLIRTLVSARRQLLKMRLQISNQIRGLMKTFGLVVPKGAGS
                     VFERNVRALLQDYEKLGRIVLPLLQAWSDLRHRTAELHKQLVMMAGEDSRCRLLMSVP
                     GVGTVTATAFAAAVEDPANFKNSRAVGAWVGLTTRRFQSGEVDYDGHISRRGDTQLRH
                     LLFEAAAVMLNRSRDTSTLRTWALGLKERLGFKRAAVALARKLAVIMHAMLRTGELFD
                     PHTGTPTAA"
     gene            complement(1821..3191)
                     /locus_tag="VKQ27_01100"
     CDS             complement(1821..3191)
                     /locus_tag="VKQ27_01100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HLY87555.1"
                     /translation="MEGRRTRPLAIGPVENTYPLRSMMQHHERPEPALVSAILEAVDH
                     LHGEQIAFLTRLVQCSSLRGAEDGAQLLVEDELARRGYGVTRVPTCRIAHDARFSPGE
                     IDYSRTWNLLARRHEGGNGRSLILNAHVDVVPEGPADRWTHAPFGGTVEGDWMYGRGA
                     GDMKAGLAAIVFALDALERAGVQPAGQVQIHAVVEEEITGNGSASLLAQGHMADAVLI
                     PEPTDEQLVRANSGVIKFRVTLQGSPAHPREPESGHSAIDLAVYLATRLKELEARWNA
                     ERVDHPGFEGFSNPAALTIGTISGGEWIASLPASCAFEGRVGFYPGDLPAERQREFER
                     FVAHVADTSDEFRGCRPPLVEWVGVCQPGYLLAPGGTAEVALDVAQRMAVETNSALRD
                     FVMPCYLDSTLYANYAGIPSLVYGPIVQNIHGFDERVSLSSLARVTKTIALFIAMWCG
                     VRRETP"
     gene            complement(3385..3852)
                     /locus_tag="VKQ27_01105"
     CDS             complement(3385..3852)
                     /locus_tag="VKQ27_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLY87556.1"
                     /translation="MDLDGIDLRILRALQSNNRITSEQLGNLAGLSPTACQRRLKRLR
                     EEKVIEGDVSIVSPRAVGRHFTTLVQVSLERERADIIDRFKASIRGAPEIMMGFYVTG
                     DVDFLLVITAKDMEDFEQFTRRFFYENPDIKGFKTMVVIDRVKAGFALPIEIP"
     gene            4158..5561
                     /locus_tag="VKQ27_01110"
     CDS             4158..5561
                     /locus_tag="VKQ27_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLY87557.1"
                     /translation="MSRTLSEIAQLDRGSVLHPFTQLKDYATGNAGDPTIVVGGEGIR
                     IKDATGREYIDGFAGLYCVNIGYGRTEVAEAISRQAHKLAYYHTYAAHTTDELAILSD
                     RLVRMAPGKMSKVFYGMSGSDANETQAKLVWYYNNLRGKPNKKKIIARERGYHGCSVV
                     SGSMTGMSFYHDHMDLPVRGILHTGVPHHYWGAEPGETEEQFSARRAKELEELILRED
                     PETVGAFIAEPVLGTGGITPPPDGYWEAIQAVLRKYDILLIADEVVTGFGRTGSLFGS
                     HHYGIEPDLITVAKGLTAAYIPLSASIVGEKVYSVMESEADRIGSFSHGYTYSGHPIG
                     AAAANAVLDIVEKEDLPGNARRVGGYFQQRLKEVFSQLPVVGEVRGVGLMGALEFVAD
                     RGKKTRFDGSLKVGARISRAARDRGLIARAMPHGDILGFAPPLVITKEEVDTIVGIAE
                     EAVRQVMDELAAEKAFA"
     gene            5743..7149
                     /locus_tag="VKQ27_01115"
     CDS             5743..7149
                     /locus_tag="VKQ27_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLY87558.1"
                     /translation="MTVNVPLLTFASEIAVASPFDGSFIASIPETPASAIGEILARAR
                     RGAVLAREMPRHQRAAILEDAAGRVGAAHEEFARLITREAGKAIRQARMEVARCINTL
                     KLSAEEAKRGAGEMIPFDSYAGSETRIGWFTREPMGIIAAITPFNDPLNLVAHKLGPA
                     IAAGNGVILKPSELAPLSALRLVDALVAAGLPAEVITTAVGGADLGAAIASTREIRMI
                     SFTGGFRTGEAIARSAGLKKLAMDLGGNAPVIVMADADLDKAVEGCVSGAFWAAGQNC
                     IGTQRILIERSVYDAFRDDFVAQAARLVAGDPSEDTTDVGPMITEAAARRAETTVAQA
                     VEAGARVLLGHKREGAVYSPTVLENVPHGTALWCEEAFAPVVTLEPFDKFDEALDLAN
                     AIDFSLHAGIFTSNLDRAMEAATRIEAGGVMINDSSDYRFDAMPFGGFKYGSLGREGV
                     RFAFEEMTQPKVVCIKRN"
     gene            complement(7622..8215)
                     /locus_tag="VKQ27_01120"
     CDS             complement(7622..8215)
                     /locus_tag="VKQ27_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY87559.1"
                     /translation="MGDERSIRSRQIVKAAIGVFLRYGYARTTMGDIAQAAGLTRPTL
                     YLSFPDKDSIFQVVVETLVADKLAEIRQGMAARETLHDKLGFACEAWGAEGFDLVRAH
                     PDAKDMFDLGFGAVCAGYGAFEEVLAELVAEPLAAAGLTMTAAELAHVLVFAIKGFKD
                     IARDGVEIRRMITMQVAIVAAALDAGGIIRDRRQGPP"
     gene            8330..9163
                     /locus_tag="VKQ27_01125"
     CDS             8330..9163
                     /locus_tag="VKQ27_01125"
                     /inference="COORDINATES: protein motif:HMM:NF006114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87560.1"
                     /translation="MADTMKVWFITGAGRGFGRLWAEAALRRGDKVAASARDPKALEP
                     LAANAGDRLLPLALDVTDRPAVFDAVSRAAAHFGRLDVVLSNAGYGVLGAVEETSFED
                     ARAIFETNVLGTLSLIQAALPILRAQRSGHILSVTSAGGLLAFPLMAVYEATKFAIEG
                     LGEALAQEVAEFGIKVTLIEPGPYKTDFMSGTSLKQSNALPVYQAMRERFATMLPAEI
                     FGDPAATADAILKVVDAESPPLRLLLGRMLPLVKQAYSERLTTWEAWDAVAQAAHGAA
                     P"
BASE COUNT         1776 a         2968 c         2955 g         1768 t
ORIGIN      
        1 acgaacgaac atgaagtaat cgcgggagcg atggccacgt agcgtggcgg tgatgttcgg
       61 atggggccgg tggctgtccc ccgataggat gaagcaacgg gctcacgatc gacaccgggc
      121 ccaggcatct agcggaggac ggccatgggg caatatgtcg gtctcgacgt atccatgaaa
      181 gagacggcaa tttcgatccg gcaggacggt aagcggatct ggcgcggcaa gtgcgcgtcg
      241 gacccgcaac tgctggcgac ggtgattcgc aagcgcgctc cgcaggcgca acgggtcgta
      301 ttcgagacgg gaccgctgtc ggtgtggttc taccatgcgc ttgtggcgga gggcttgccg
      361 gcgatctgta tcgacgcacg acatgccaag gccgccctcg acatggccgc gaacaagacc
      421 gatgcgaacg atgccgatgg tctggcgcag ttggccgagg tgggcttcta ccgagaagtc
      481 cgggtcaaag gcttcgacag tatgctgatc cgcacgctgg tctccgctcg gcgccagctt
      541 ctcaagatgc gattgcagat atccaaccag atccgcgggt tgatgaagac attcggtctc
      601 gtcgtaccca agggtgccgg tagcgtgttc gagcgcaacg tccgcgccct tttgcaggac
      661 tatgagaagc tggggcgcat cgtcctgccg ctccttcagg cttggagcga tctccggcac
      721 cggacagccg agctccacaa gcagttggtg atgatggctg gcgaggattc gcgctgccgc
      781 ctgctgatgt cggtgcccgg tgtcgggacc gttaccgcca ccgcctttgc cgcggccgtg
      841 gaggatccag cgaacttcaa gaactctcgc gctgtcggcg catgggtggg cctgaccacc
      901 aggcgcttcc agtccggcga ggtcgattac gatggccaca tctcgcgtcg tggtgacacc
      961 cagttgcggc acctgctctt cgaggcggcc gcggtcatgc tgaaccgatc gagggacaca
     1021 agcacattgc gaacctgggc gttggggctg aaggaacggc ttggcttcaa gcgagctgcc
     1081 gtggcccttg cacgtaagct ggcggtgatc atgcacgcga tgctcaggac gggcgagctg
     1141 ttcgacccgc acacaggaac gccgaccgca gcctgatctt ggtgtcagcg cctggcaagc
     1201 gctcgcctct aacgaacttc atcaggccgc tccaccgata gcgccccttt gggacgcgcc
     1261 gagccgtccc tgccgggacg tggttgagat cattccgcga agcgggaagc aactgcctcg
     1321 tttagcagat tgcgtcacga acataggaag atctcacctg cggaactcat cctgcggcga
     1381 tccgcttcgc atcgaccgcg tagacgaccc tgtacccggc atacgatcca acatcggctg
     1441 gaatgagtaa cttggacctt cctcttgacg gcggagcgat tagacgaccc gcttcgcggg
     1501 atgggtgccg cggttgggtc ggcacgtatt cgccgaggtc ttgagcgggt gagatagtct
     1561 tttgagcatg gagtttcctt catcttgaga ggaacaccga actcagcaga gcgtgtaatc
     1621 cataggtaat cgcaagcttc accggatggc tacgttgcgg cacgccgcat cgagatcaat
     1681 agcagggtaa ccggacgccg agtgagttcg gatttgtgtt cttgcgcggg aaaacatagt
     1741 ggcgcgcgcc tcggtcaatg ccctccgcgg cgggatagac tggtggggaa ggcagccgga
     1801 ccgcattggc tcgacggcga tcaaggggtc tcgcgtcgca ccccgcacca catggcgatg
     1861 aacaatgcga tggtcttcgt gacgcgggcc agcgacgaga ggctgacgcg ttcgtcaaag
     1921 ccgtgaatgt tctgaacaat cggcccatag acgagcgagg ggatgcccgc atagttggca
     1981 tagagcgtcg agtccagata gcagggcatc acaaagtccc gcagggctga atttgtctcc
     2041 acggccatcc gctgcgcaac gtcgagcgct acctcggctg taccgccggg ggcgagcaaa
     2101 tagccgggtt gacagacccc cacccattcg accagcggcg ggcggcagcc gcggaattcg
     2161 tcactcgtat ctgcgacgtg ggcgacgaaa cgttcgaatt cgcgctgacg ctcggcaggc
     2221 agatcgcccg gatagaagcc gactcgcccc tcgaacgcac aggacgcagg caacgacgcg
     2281 atccattctc cgcccgatat cgtgccgatg gtgagcgctg ctggattgct gaagccctca
     2341 aaccccggat ggtcgacgcg ttcggcattc cagcgcgcct caagctcttt caggcgtgtc
     2401 gccagataga cggcaagatc gatggcactg tggccgcttt caggttcgcg cggatgggcg
     2461 ggggaaccct gaagcgtcac gcggaactta atgacgccgc tgttggcacg gacaagctgt
     2521 tcatccgtcg gctcggggat cagtaccgca tccgccatgt ggccctgcgc cagcaggctg
     2581 gccgagccgt tgccggtgat ctcctcttca actacggcgt ggatctggac ctgccccgcc
     2641 ggttgaaccc ccgctctctc cagcgcgtcg agcgcgaaga cgatggccgc cagccccgcc
     2701 ttcatgtcgc ccgcgccgcg gccgtacatc cagtcgcctt cgacggtgcc gccgaagggg
     2761 gcgtgcgtcc aacggtccgc cggcccctca ggcacgacat cgacatgggc attgaggatc
     2821 agcgagcggc cgttgccgcc ctcgtgccgt cgggccaaaa ggttccaggt ccggctgtag
     2881 tcgatctcgc cgggtgaaaa gcgggcgtca tgcgcaatcc ggcacgtcgg cacgcgcgtg
     2941 acgccataac cgcgccgcgc cagttcatct tctacgagaa gctgggcgcc atcttcggcg
     3001 ccccggagag agctgcattg caccagccgc gtcagaaatg cgatctgctc accgtgcaga
     3061 tgatcaacag cttcaaggat cgcggaaacg agagcgggtt cgggccgttc atggtgctgc
     3121 atcattgacc tcagtgggta cgtattttcg acaggcccga tggctagtgg tctcgtccgg
     3181 cggccctcca tcgcggagac aaggatctga tttctgaaac gttcgccgag ctaaccttgg
     3241 ccttggcgct gataaatcta gcgccaagcc cgtcataaat ccggcaaatg tcgcccttct
     3301 gacgcagtat tctcgcgcaa attgtcacga aattgcaggg agtacgcgca tggccagcgt
     3361 cgagctgcga attgcgcaaa ccgctcaagg gatctcgatg gggagggcga agccggcttt
     3421 gacacgatct atgacgacca tggtcttgaa gcctttaatg tcggggttct cgtaaaagaa
     3481 gcgccgggtg aactgctcga aatcttccat gtccttggct gtgatcacca gaaggaagtc
     3541 gacatccccg gttacataga accccatcat gatttcgggc gcgccgcgga tcgaggcctt
     3601 aaaccggtcg atgatgtccg cccgctcgcg ttccagcgag acctgaacga gcgtcgtgaa
     3661 atggcgtccg acggcgcgcg gtgagacgat ggaaacatca ccctcgatca ccttctcctc
     3721 ccgtagccgc ttgagtctgc gctggcatgc cgtgggcgac aatccggcga ggttgcccag
     3781 ctgttcgctg gtgatccggt tgttactctg caatgcccgc agaatccgca gatcaatgcc
     3841 atcgagatcc attgcgcccg aatccttcat gtttagctgc aacattgcag gattttcgca
     3901 taattgagcc gaattccgcc gtcaatcgta cccggcacaa tcgtattttc cttgccaggc
     3961 gggcaccaac ggccgccgcc tgacaacagt gcggacaatg aactcggcaa tacatgtgat
     4021 cggcggcgag caagatcaga ctactgctgc ctgctccatc gagtccgctt ctgaggttga
     4081 gcgacgcggc gacgggtctg tggcctcggt cccagatggc agccgcgcca gaaagcaacg
     4141 ttagccagga gctgctcatg agccgtaccc tctcagaaat cgcccagctg gaccgcggca
     4201 gcgtcctgca tcccttcact cagttaaagg actacgccac cggtaatgca ggcgatccga
     4261 ccattgtcgt gggcggcgaa ggcatccgca tcaaggacgc gaccggacgc gaatacattg
     4321 acggcttcgc gggtctttac tgtgtgaaca tcggctacgg ccgtaccgaa gtggccgagg
     4381 cgatcagccg tcaggcccac aagctagcct actatcatac ctatgccgcg cacacgaccg
     4441 atgagctcgc aatcttgtcg gaccgcctcg tccgtatggc gccgggcaag atgagcaagg
     4501 ttttctacgg catgtccggc tcggacgcca acgaaaccca ggcgaagctg gtgtggtact
     4561 acaacaacct gcgcggcaag ccgaacaaga agaagatcat tgcccgcgag cgcggttatc
     4621 acggctgcag cgtcgtgtca ggctcgatga ccggcatgag tttctatcac gaccacatgg
     4681 atctgccggt gcgcgggatc ttgcacacag gcgtgccaca tcattactgg ggcgcggagc
     4741 ccggcgagac cgaggagcag ttctcggccc gtcgtgcaaa agagctggaa gagctgattc
     4801 ttcgcgaaga tccggaaacc gtcggcgctt tcatcgccga gccggtcctg ggcacgggtg
     4861 gcatcactcc tccgccggac ggttattggg aagccatcca agccgttctg aggaaatacg
     4921 acatcctgct gatcgcggat gaggtcgtga cgggcttcgg ccgtaccggc tcgctcttcg
     4981 ggtcgcatca ctatggtatc gagcccgacc tgatcaccgt cgccaagggc ctgaccgccg
     5041 cctatattcc gctctcggcg tcgatcgtcg gtgagaaggt ctattcggtg atggaatccg
     5101 aagcggaccg catcggctcc ttctcgcacg gctacaccta ttcgggtcac cctatcggtg
     5161 cggcggccgc gaatgccgtg ctggatatcg tagagaagga agacctgccg ggcaacgcga
     5221 ggcgcgtcgg cggttacttc cagcaacgtc tgaaggaagt cttttcccag ctgcctgtcg
     5281 tcggcgaagt gcgcggcgtg ggcttgatgg gtgcgttgga gttcgtcgcc gatcgcggca
     5341 agaagacccg cttcgacggc agcctgaaag taggcgcgcg catttccaga gccgcgcgtg
     5401 accgaggcct aattgcccgc gccatgccgc acggggatat cctgggcttc gccccgccgc
     5461 tggtaatcac caaggaagag gtcgacacga tagtcggcat cgccgaggaa gccgtccgtc
     5521 aggtgatgga cgaacttgcg gccgaaaagg cattcgcctg aagccaaaga cgggagggcg
     5581 ggtggccccg ccgtcccgat cgatatcata cgaccgaacc ccggcgagcc ggcctcaacg
     5641 ccgtcgtcca tcccgcccct ctgtcttcgc ctgtcggcgc agagcccggt cggcttgtcc
     5701 cctcaccttg cttcaaggtc cgcctaccga ggagagaccg acgtgacggt taatgtcccc
     5761 cttttgactt ttgccagcga gatcgccgtg gccagccctt tcgatggatc gttcatcgca
     5821 agcattcccg aaaccccggc ttcggcgatc ggagaaatac ttgcccgcgc tcgccgtgga
     5881 gccgtgctgg cgcgcgaaat gccccgtcat cagcgcgctg ccattcttga agacgcggct
     5941 ggccgggtcg gcgccgcgca tgaggagttc gcccgcctga tcactcggga agccggaaag
     6001 gctatccgtc aggctcgcat ggaggtcgca cgctgcatca acacgctgaa gctgtcggcc
     6061 gaggaagcca aacggggcgc gggcgaaatg atcccctttg attcttacgc cggctccgag
     6121 acccgcatcg gctggttcac ccgcgaaccg atgggcatca ttgccgcaat cacgcccttc
     6181 aacgacccgc tgaatcttgt cgcgcacaag cttggcccgg ccatcgcggc cggtaacggc
     6241 gtgatcctga agccgtcgga actcgccccg ctatcggctc ttcgtctggt cgatgcactg
     6301 gtcgccgccg gccttcctgc ggaagtgatc accaccgccg tgggaggggc cgatctgggt
     6361 gccgcaatcg cctcgacccg cgaaatccgc atgatctcgt tcacaggggg cttccgcacg
     6421 ggcgaagcca tcgcccgatc ggcggggctg aagaagctgg cgatggacct tggcggcaac
     6481 gcgcccgtca tcgtgatggc cgacgccgat ttggacaaag ctgttgaggg ctgcgtctcc
     6541 ggcgcgttct gggctgcggg tcagaattgc attggcacac agcgcatcct gatcgagcgt
     6601 tcggtctacg acgcgtttcg cgatgatttc gtcgctcaag ccgcgcgctt ggttgcaggc
     6661 gatcccagcg aagacacgac cgatgtcggc ccgatgatta ccgaagccgc ggcccggcgc
     6721 gctgaaacga ccgtcgcgca ggctgtcgag gcgggcgcgc gcgttctgct gggccacaaa
     6781 cgcgagggcg ccgtgtattc gccgaccgtg ctggagaacg tgccgcacgg gaccgcgcta
     6841 tggtgtgagg aagccttcgc cccggtcgtc acgctggagc cattcgacaa gttcgacgaa
     6901 gcgctggacc tcgccaacgc catcgacttc agtctgcacg caggcatctt cacctcaaac
     6961 cttgaccgag cgatggaagc tgctactcgc atcgaggcgg gtggcgtaat gattaacgat
     7021 tcctccgact accgcttcga cgcaatgccc tttggcggct ttaagtacgg cagccttggc
     7081 cgcgagggcg tgcgtttcgc cttcgaggaa atgacccagc ccaaggtcgt ctgcatcaaa
     7141 cgaaactgat ggctgatcgc ggcgccgtcg tttcggcggg ggcgaggccc tgcgttccgc
     7201 ctgcgccacc ctgtcaccgt gtgcatagcc tgggggaatg atgagccaac ctgctcaccg
     7261 cattgaacac gaccttctgg gcgatcgcga gcttccgggc agcgcttaca ccaccacggt
     7321 cggcacggct ttcctacaaa aacgattacc tcagaaaccc ggaaaatgcc gccagatcaa
     7381 tcgcctatcg attgatgacg ggcgacattg tagttgtcga tcctttcgac cgcttgcccc
     7441 tatgggtagc gctgtctcgc accaccgatc cgatgcgcgt cgccagcaat ctcaggatct
     7501 tcgatttcga gctcaatgcc gccaaaatgg cggcaattca tggtcttgcc cgggccagca
     7561 gccgcatctt cgatccctcc ggccttgccc cggtgtggga ccccacatcg tccgtcagcc
     7621 gtcagggggg gccttgcctc cgatccctga tgatgccgcc ggcatcaagg gcagcagcca
     7681 cgatggcgac ctgcatcgta atcatgcggc ggatctcgac gccgtcccgg gcgatgtcct
     7741 tgaagccctt gatcgcgaag acaaggacat gcgccagctc cgctgcagtc atcgtgagtc
     7801 ctgcggcggc aagcggctca gccaccagct cggccagcac ctcctcgaat gcgccatatc
     7861 cggcgcagac cgcgccgaat cccagatcga acatgtcctt cgcatccgga tgtgcgcgca
     7921 cgagatcgaa tccctcggcg ccccaggcct cgcaggcgaa gcccagcttg tcatggagcg
     7981 tctcgcgcgc ggccatgccc tgtcggatct cggcaagctt gtccgcgact agcgtctcta
     8041 cgacgacctg gaagatgctg tccttgtccg ggaaagagag atagagcgtc ggccgcgtca
     8101 gcccggccgc ctgcgcaatg tcgcccatgg tggtgcgcgc atagccgtag cgcagaaaga
     8161 caccgatcgc tgctttaaca atctgccggc ttcgtatcga gcgctcgtcc cccatgccgg
     8221 catcctgaca cattgactct gtgtgtcaat gcgacgacca ttgacgaatt gacgcaatat
     8281 gtcagctcgt agccaaagca cattcgatcc ttaatcgagg aggttcatca tggccgatac
     8341 catgaaggtt tggttcatca ccggcgcagg gcggggattc ggccgtcttt gggcagaggc
     8401 cgcattgcgt cgcggtgaca aggtcgcggc gtccgcgcgc gatccgaagg ctctggaacc
     8461 gctggccgcc aatgccggcg accgtctcct cccgctcgcg ctcgacgtga ccgaccgtcc
     8521 cgccgttttc gatgcggtga gccgggcggc tgcgcatttc gggcggctcg atgtcgtcct
     8581 aagcaatgcc ggctatggtg ttctgggcgc ggtcgaggag acgagcttcg aagacgcccg
     8641 cgccattttc gagacaaatg tcctgggcac gctgtcgctg attcaggctg ccctgcccat
     8701 cctccgcgca caacggagcg gccacatcct ctcggtcacg agcgccggcg gcctactggc
     8761 ttttccgctg atggccgttt acgaggcgac gaaattcgca atcgagggtc tgggcgaggc
     8821 cctggcccag gaggtcgcgg aattcggcat caaggtgacg ctgatcgagc ccggccccta
     8881 taagaccgac ttcatgtccg gtacctccct gaagcagtcg aatgcgctcc ccgtttatca
     8941 agcgatgcgc gagcgcttcg cgaccatgct gccggccgaa atattcggcg acccggcggc
     9001 cacggctgac gcgatcctca aggtcgtcga tgcagagtcc ccgccgctgc gcctgctgct
     9061 cggccggatg ctgccgcttg tgaagcaggc ctatagcgag cgcctgacaa cctgggaggc
     9121 ctgggacgcc gtcgcgcaag ccgcacacgg cgccgcgccc tgacgcagga ctcgtgagcg
     9181 atcggcgacg gcagcgggtt ggtggaacgg tggaacatac cgtacttggg ctcgccgccg
     9241 agatcggccg ctattcccgg aaagcgaggg ccggagcggg tcggcttcgg ccctggactt
     9301 ggcaaaagcc atcgcgattg gaatctacta gcactacttt ggcactttag gtttcgcggc
     9361 ctccgcaagg ggcatgtcac agtgcggaca tcgtcggcgt ggagatcgtg cgaagaggtc
     9421 gaggatcgct tgcctgatgg ccggcatgct cggttgtggt ggtggtc
//
LOCUS       DATGEM010000043        13031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1240438, whole genome shotgun sequence.
ACCESSION   DATGEM010000043 DATGEM010000000
VERSION     DATGEM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240438"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..802
                     /locus_tag="VKQ27_01130"
     CDS             56..802
                     /locus_tag="VKQ27_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87561.1"
                     /translation="MGFEFSNKRAVVCGGSRGIGRSIALGLAQAGAAVSICARGVETL
                     QATRAEIAAFGHTSHAGVCDLGDGAAIRRYIADAAQTLGGIDILVNNASGFGSSDDEA
                     GWAVSVAVDMMAIVHATQAALPFLKEAKGNVVNTSSISAMRTSARQPPYGAIKAAVIH
                     YTMTQAAMYAKDGIRVNGVAPGSIEFPGGVWDRRKSDNPNLYNAILRSIPFGRLGHPE
                     EVANAVLFLASPLASWITGQTIVVDGGQLL"
     gene            complement(759..1688)
                     /locus_tag="VKQ27_01135"
     CDS             complement(759..1688)
                     /locus_tag="VKQ27_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87562.1"
                     /translation="MPIRLSENFRALFYAPFYAAHAIGAYEAEDVDVTLVSSPDPART
                     ATSLHDGEVDVMWGGPLRVMLTHARDVGADSVCFCDVIARDPFFVIGREPRPEFQLAD
                     LASMRFASVAEVPTPWLCLQDDLRRAGIDPASFNRIHGPTMAENAAALRTGRLDAVQL
                     FQPYAEELILSGEGHVWYAAASRGLTAYTTLVTRRAMLQSRSEELLRMTRAMARVLRW
                     IEATPGSEIAGALADYFPSVPGNIFAAAMDRYRALGLYATDPVMPPEGFERLQAAMRS
                     GGALDRAIPFEDCVDNTLALIATGRHPPLSSGR"
     gene            complement(1800..3179)
                     /locus_tag="VKQ27_01140"
     CDS             complement(1800..3179)
                     /locus_tag="VKQ27_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87563.1"
                     /translation="MSSSTAHDGSRAAASDAVNHGAQLRRAVIASAIGTTIEWYDFFL
                     YSTVTGLVFAKLYFPKADPLVGTLEAFAVYAVGFAARPIGAAIFGHWGDRLGRKSALI
                     ATLILMGVATFLVGIVPTYASIGIWGAVLLTLLRFIQGIGVGGEWGGSVLLSMEWART
                     DHHRGFVASWPQFGVPAGLFLANLAVLVFSAMSGDQFSSWGWRVPFLLSIVLVAIGLW
                     IRLGILETPVFRRLIEEDRIERAPVAEVFRLQAKEVFLSAFVRMAEQAPFYIFTAFVF
                     AYGVGTLKVSRDLLLTAVLVASVVSFFSIPIAGHLSDRFGRRRVYIVGAIATGVFGWI
                     YFALLNTLVAFWIFAAIVVSLIPHDAMYGPQAALIAESFTGRLRYSGASIGYQLASII
                     AGGPAPLIAAALLASYGSWVAIAVYISICAVISVIAAALMQDYTGKDISAEYAETDVI
                     ASAAGESRM"
     gene            3281..3790
                     /locus_tag="VKQ27_01145"
     CDS             3281..3790
                     /locus_tag="VKQ27_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87564.1"
                     /translation="MNLRIVVVNDRMQNGYRYECVAPMGRNFDPGFTPDLTPREMLEL
                     GVFCGKYMTDTCDEFPASWFRHARLSPERRDCSLNFFGIDASQPLSIWRAKGWIHPDD
                     PRGWFQWYCRYYMGRRTPQEDARQIARWKAIRRHVGQIKRNCEPGDLDCRKRQRQALL
                     HWAYDSRKI"
     gene            complement(3794..5002)
                     /locus_tag="VKQ27_01150"
     CDS             complement(3794..5002)
                     /locus_tag="VKQ27_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87565.1"
                     /translation="MTRNARLITFVNAAHTVTHYSLLILPTAVLAMATPGGRFGGAYG
                     PILALATGMFVLYGLFSLPQGWIAQRVGRPALMTVFFVGTGLSMVATAFASSPVTLAL
                     TLAATGLFAAIYHPIGTAMLVDAAGDKPGRAIGINGVFGNVGVALAPVATAFLASQVG
                     WRAAFLVPGLVCVGLGIAWLRLPAPEATAYHAARVFPDIPRHLVRRAVIVLLLIAVVS
                     GLVFNAFTLLLPKLIEERLGNDPRMLPLVGSAAFAATLCGALTQFTVGRMIDRMTLRR
                     VFLPISVLLVPGLVAVSYAQGWVVVPMAALVSAAIFGQVTVNETMTARYISPALRTRM
                     YSVRFFVGFLGAAAAAPVVAWLHERTGSLTLVTLVMAGFGVITLGCALAFPDRREELH
                     PELWAVVPAE"
     gene            5094..5846
                     /locus_tag="VKQ27_01155"
     CDS             5094..5846
                     /locus_tag="VKQ27_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY87566.1"
                     /translation="MRPFEEAPRPLVGYAYDYASAHDTNWHQHPRAQLLHATSGVMRV
                     ATQSALFIVPPGTGLWMPARTPHVTRMPTGLAMRALFLREDAARAGPDMVTVVSVSPL
                     LRELILAACEQPVMWDEDGPVRHVVALALHEIGHAATRPIAVPACRDPRLMRVTEALL
                     ADPSDTRGLEAFAETAAASSRTLARLFRKETGMSFQEWRRQLRLTEALASLAQGATPA
                     RAAATVGYTSGPAFGAAFRGAFGITPGQSRRG"
     gene            complement(5832..7679)
                     /gene="recQ"
                     /locus_tag="VKQ27_01160"
     CDS             complement(5832..7679)
                     /gene="recQ"
                     /locus_tag="VKQ27_01160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HLY87567.1"
                     /translation="MLQSLLHVQTRDEPADVLRRVFGFPGFRGQQEEVVRHVVAGGDA
                     LVLAPTGGGKSICYQVPALCRRGTAVVISPLIALMEDQVAALRQLGVAAGALHSELDP
                     SEARSVARDLIEQELDLLYVSPERLLANGMLDRLSRLPLALFAIDEAHCVSQWGHEFR
                     PEYRALACLADRFAGVPRVALTATADPRTRADILQALRMDTAGVFISSFHRPNLQIGA
                     QPKVGETAQLLELLSRHHGACGIVYCATRAKTERVAARLVDKGLPAVAFHAGLDAEHK
                     RAALTRFRSGEPIVVVATIAFGMGIDRPDVRFVVHLDMPDSPEAYYQQIGRAGRDGDP
                     SETLLLYGGQDIAQARHWLAQSAAPESQKRVMRTKLEEMIALTEAVTCRTRSLLACFG
                     EDLPEPCGHCDNCAVPPRQVDATVEAQKVLSAVYRTDQLFGAIHIIAVLRGEMTEGVV
                     RHAHDRLQTFGVGADHPAQYWRGLIRQLIALGALDVDTAGHGGLFLVQEKARPILRGE
                     TRVVLREEGRRPRDVLRPTRTAAAKDDSGAEVQTLFEALRSWRAAEAKAQRIPPYVIF
                     HDTVLREIAAVRPVSVEELGQIKGVGASKMQRYGTAVLGVIGGLALAPS"
     gene            complement(7683..8072)
                     /locus_tag="VKQ27_01165"
     CDS             complement(7683..8072)
                     /locus_tag="VKQ27_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLY87568.1"
                     /translation="MRRLGLLLLLLFAATTSTCAADPPVQKFVVFFGDWSAAIDNNAQ
                     TVIGNAAAWVKSHPGNVAHVNGFADPTGSKEANILLSELRAQVVVDQLQTDGVAPGEV
                     QQTGHGSVQFALTSQESRRVEISISRP"
     gene            complement(8170..9786)
                     /gene="cydC"
                     /locus_tag="VKQ27_01170"
     CDS             complement(8170..9786)
                     /gene="cydC"
                     /locus_tag="VKQ27_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HLY87569.1"
                     /translation="MNDVLRILALWRGRAVWLAIGLFVSLAALASGVGMMAVGGALLA
                     GGVLAASIALRGLGSTRVVLRYAERVSTHDATFRALADLRVWFFRNLARGAAGGLGFR
                     QAGDVLARLVTDIEALDGIYLRILVPLAGAVVLLPVLIVVIGLHSVGLALGIGVLFAV
                     SAFVLPWFAARMAARAAARLAGAIAALRITALDALTGLREVRAFGAEGRMLAAVQARE
                     ASLLTTQHELASRSALANAGAFLCGQMAILAVLVAAGMNPAAAVIAAFLVVASFEAVG
                     GLPRAGVLAGHAAAAAHRVLQAAEAPVAVPDPPQPGVLPRGFALRLEAVHFRWQPDRA
                     PVFEGLSLEVPQGARVALLGPSGAGKSTLAALALKVVAPQQGKVTLGGIDIATLGAAD
                     VRGRFGWLSQATHLFDDTIRANLKLASPDADDAVLWAALDAAHIGEMVRALPDGLDTW
                     VGEGGARFSGGQGRRLALARALLSPAPILILDEPCAGLDAETERAFLATLNEVAADRS
                     VILITHRLIGVERLDRIWRLSGGKAIAAAA"
     gene            complement(9783..11468)
                     /gene="cydD"
                     /locus_tag="VKQ27_01175"
     CDS             complement(9783..11468)
                     /gene="cydD"
                     /locus_tag="VKQ27_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HLY87570.1"
                     /translation="MSSEHGKAASRAWMRSEQKIGARAARPVMLLNLLGVALAIGQAW
                     FAGSVLAAALTRHAGKTAPFMVGFAMLALVRAAVSIVAEKAAFNAGAAARRRLRTDAL
                     ARLLHAGPSQLRVQHTGALTATVVDRIEALDGLYARWLPTAMLAIAGPVLVALAALLV
                     DPFAALVLFLCGLMVPVAMAAAGIGAAAASRHQFLALARLQARFLDRVRGIATIVLYG
                     QAEAEARSLATAADELRRRTMRVLRVAFLSSAALDVAAALAFVVLAIRYGSQLLAGGL
                     AQPAAALFVLLLVPEFFAPLRAFAAAYQDRLHAAGAAEALTELPPAPEPQPQREIRTV
                     AAQGVTVAFEDVYLTWDPSRGPALNGLSFRLPAGETLVLAGPSGAGKSTAIEILLGFV
                     RPDRGRVTINGADIADLVPQALARLTAWLGQRPMLFSGTIRDNIRFARPEATDAEIEA
                     AARSASVSEFAAALPDDLDTQVGEGGYGLSGGQAQRVAIARAFLKNAPLLLLDEPTAH
                     LDPATEADVLDSLRRLALGRTVILASHSSAAHGFGGRRLDIRDGRATPARGAA"
     gene            complement(11549..12295)
                     /locus_tag="VKQ27_01180"
     CDS             complement(11549..12295)
                     /locus_tag="VKQ27_01180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HLY87571.1"
                     /translation="MQLDGQIALVTGGSRGIGRATALAFAAEGADIAFCHLNDGAKAD
                     DTAAEIRALGRRAMHRSLDVVDIAASRGFAQEVVAAFGPIDILFNNAGINIRKKFEDY
                     TEADYDRILDVHMKGMFFMAQAVYPAMAARGRGCIINVASQRGLKGAPNSAPYCAAKA
                     AIIGFTRALACEAAPKGVRVNAVAPGPIVTDLTATMEPADRQAFIDALPVHRFGRPEE
                     IAATALLLAGPHGAFYVGATLSPNGGDVMY"
     gene            12428..>13031
                     /gene="panC"
                     /locus_tag="VKQ27_01185"
     CDS             12428..>13031
                     /gene="panC"
                     /locus_tag="VKQ27_01185"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLY87572.1"
                     /translation="MDLARTIDDLHSACTALRQRFGALALVPTMGALHKGHIALVRAA
                     AASGAAVVTSIFVNPLQFGATEDLTRYPRDEAGDLAILRRVGCHLVWLPDVATMYPAD
                     AVTTIDLAGPAEQWEGTARPGHFRGVATVCAKLFGQVGAGRAYFGEKDWQQLQVVTRM
                     VADLHLPLEIVGVPTVRDPDGLALSSRNRFLTQQERARAPR"
BASE COUNT         2536 a         4448 c         4053 g         1994 t
ORIGIN      
        1 cacgcggcgg gtctttgcag actcccagcc cgatcgcaac gagggaaagt gtcccatggg
       61 gttcgaattc agcaacaagc gtgccgttgt gtgcggcggc agccgcggca tcgggcggtc
      121 catagccctc gggcttgccc aggcaggcgc cgccgtctcg atctgcgcgc gcggcgtcga
      181 gacgttacag gccacgcgcg ccgagatcgc ggcatttggc cacacgtccc acgccggtgt
      241 gtgtgatctg ggcgatggag cggcgatccg gcgctacatc gcagacgcgg cacagacgct
      301 gggaggcatc gatatcctgg tgaacaacgc gtccggtttc ggctccagcg atgatgaggc
      361 gggctgggcc gtcagcgtcg ccgtggacat gatggcgatc gtccacgcca cccaggcggc
      421 tctgccgttc cttaaggaag cgaagggcaa cgtggtgaat acatcgtcga tctcggcgat
      481 gcgcacgtcg gcgcggcagc caccctatgg tgcgatcaaa gcggcggtga tccactacac
      541 gatgacgcag gcagcaatgt acgcgaaaga cgggatccgc gtgaatggtg tggcacccgg
      601 ctccatcgag tttcccggcg gcgtctggga tcggcgcaag agcgacaatc cgaatcttta
      661 caacgctatc ctgcgctcga ttccattcgg caggctgggg cacccggagg aagtagcgaa
      721 cgctgtgctg ttcctcgcgt caccgctcgc gtcgtggatt accggccaga cgatagtggt
      781 ggatggcggc cagttgctat gagcgcgagc gtgttgtcca cgcagtcctc gaacgggatg
      841 gcgcggtcca gtgcgccacc cgaccgcatc gccgcctgta gccgctcgaa gccttcgggc
      901 ggcatcactg ggtcggtcgc atacagaccg agcgcacgat agcgatccat ggccgcggcg
      961 aagatgttgc ccggaaccga cgggaagtaa tctgccaggg caccggcgat ctcgctgcct
     1021 ggcgtggcct cgatccaccg cagcacgcgt gccatcgccc gcgtcatgcg cagtaactct
     1081 tcgctgcggg actgcagcat ggcgcggcgg gtgaccaggg tggtgtaggc ggtcaggccg
     1141 cggctggccg cggcatacca gacatggccc tcgcccgaga ggatcagctc ttcggcataa
     1201 ggctgaaaaa gctgcacggc atcaagccta cccgtccgca gtgccgcagc gttttctgcc
     1261 atggtcgggc cgtgaatgcg attgaatgag gcgggatcta tgcccgcgcg gcgaagatca
     1321 tcctgcaggc aaagccatgg cgtcggcacc tcggcgacag acgcgaaacg catgctggca
     1381 aggtcagcca actggaattc ggggcgaggc tcacgaccga tgacgaagaa tggatcgcgc
     1441 gcgatcacgt cgcagaagca gacgctgtcg gcgccgacgt cccgcgcgtg cgtcagcatc
     1501 acccgcagcg gccctcccca catgacgtca acctctccgt catgcagcga tgtggctgtg
     1561 cgtgcggggt ccggcgaaga taccagcgtg acgtcgacgt cctctgcctc gtacgcgccg
     1621 atggcgtggg cagcatagaa cggcgcgtag aacaaagcgc ggaagttttc cgacagtcga
     1681 atgggcatga ggaatgctgt cactgtcgcg gagacgacgc aaatccgcgg agccaggggt
     1741 cgttagctcg ctcctgtcat tgcaacgccg aagtcgctgg ggaagcaaac ttgggccagc
     1801 tacatccggg attcccccgc cgcgctcgca atgacatcgg tctcggcgta ttcagccgat
     1861 atgtccttcc cggtatagtc ttgcataagc gctgccgcga ttacgctgat gaccgcacat
     1921 atactgatat agacggcgat cgccacccac gatccgtacg atgcgagtag cgccgcggcg
     1981 atcagtggcg caggccctcc agcgatgatt gacgcgagct ggtagccgat cgacgcacct
     2041 gaataacgta accgcccggt aaagctttcc gcgatcaagg ctgcctgcgg cccgtacatc
     2101 gcgtcgtgcg gaatgagcga caccacaatc gcggcgaaaa tccagaatgc caccagcgta
     2161 ttgagcagcg cgaagtagat ccaaccgaac acgcccgtgg cgatcgctcc tacaatatag
     2221 acgcgccgcc gcccgaaccg gtcggacaga tggccagcga tcgggatgga gaagaaggac
     2281 accaccgacg ccaccaacac tgccgtcagc agcaggtcgc gcgacacctt cagcgtgccg
     2341 acaccatagg cgaagacgaa ggcggtgaag atatagaacg gcgcctgctc ggccatgcgc
     2401 acgaacgcgc tgaggaacac ctcctttgcc tgcaggcgga acacctcggc gacgggagcg
     2461 cgctcgatgc ggtcttcctc gatcaggcga cggaacaccg gcgtctcgag gatcccgagc
     2521 cgtatccaca agccgattgc caccaggacg atgctcagca ggaagggcac gcgccaaccc
     2581 cagctgctga attggtcgcc agacatcgcg ctgaacacca ggacagcgag attggcgagg
     2641 aacagccccg ccggcacacc gaactgcggc caggaggcga cgaagccgcg gtggtggtcg
     2701 gtgcgcgccc actccatgga cagcagcacc gagccgcccc attcgccgcc cacgccaatt
     2761 ccctggatga agcgcagcag agtgagcagg accgcgcccc agatgccgat gctggcatag
     2821 gtcggcacga taccgacaag gaacgtcgcc acacccatca ggatcagcgt agcgatcagg
     2881 gccgacttgc ggccgagccg atcaccccaa tggccgaaga ttgccgcgcc gattggccgc
     2941 gcggcaaacc cgacggcata aaccgcgaac gcttccaacg tcccgaccag cggatcggct
     3001 ttcgggaaat acagtttggc gaataccagg ccggtcactg tgctatacag gaagaagtcg
     3061 taccactcga ttgtcgtgcc aatggcgctg gcgatgacag cacgccgaag ctgggcacca
     3121 tgattcaccg cgtcggacgc ggccgcccgc gatccatcat gtgccgtgct gctcgacatg
     3181 gtgtcacctc gagtgccgtg gggtaaaatg ggctagtgaa cagaaacgcc caaggcaacc
     3241 cgatgcacat catccgacac cgtcactttg tcgcgacgac atgaacctcc gcatcgtcgt
     3301 tgtgaatgac cggatgcaga acggctatcg ctatgaatgc gtggcgccga tggggcggaa
     3361 tttcgatccc gggttcacgc ccgacctgac accacgcgag atgctggaac tgggcgtgtt
     3421 ctgtgggaaa tacatgaccg acacgtgcga tgaattcccg gcaagctggt tcagacatgc
     3481 caggctatcg ccggagcgcc gcgactgttc gttgaacttc ttcggcattg atgccagcca
     3541 gccgctctcg atttggcgag caaaaggctg gatccatccc gacgatccac gcggctggtt
     3601 ccagtggtac tgtcgttact acatgggccg ccgcacgcca caggaagacg cacggcagat
     3661 cgcccgttgg aaagcgatcc gccggcatgt cggccagatc aagcggaatt gcgaaccggg
     3721 cgacctggat tgccgcaagc gccagcggca ggcgctgctg cattgggcct acgacagccg
     3781 caaaatctga ttgctactcg gcgggcacca cggcccacaa ttccgggtgc agttcctcgc
     3841 ggcgatccgg gaacgccaac gcgcaaccca gtgtaatgac accgaacccc gccatcacca
     3901 aagtgaccag cgtcaggctg cccgtacgct catgtagcca cgcaacgaca ggtgccgcgg
     3961 cggcggctcc aaggaacccg acgaagaacc gcacggaata catgcgggta cgcagtgcgg
     4021 gcgaaatata gcgtgccgtc atcgtctcat tcacagtcac ctggccaaag atcgctgcag
     4081 acaccagcgc ggccatcggc accaccaccc atccttgcgc gtaggaaaca gcaaccaggc
     4141 ctggcaccag cagaacgctg atcggtagga acacgcgcct caacgtcatc cgatcaatca
     4201 tgcgcccgac ggtgaactgc gtcagtgcgc cgcacagcgt agccgcgaac gcggctgaac
     4261 ccaccaacgg caacatgcgc ggatcgttgc ccaggcgctc ctcgatcagt ttcggcagca
     4321 gcagcgtgaa ggcattgaac accaggccag aaacgaccgc gatgagcagc agcacgatca
     4381 cggcgcggcg caccaggtgc cgcggaatat cgggaaacac gcgcgccgcg tggtacgcgg
     4441 tggcctcggg cgcaggtaga cgcagccacg caatgcccag gccaacacag accaggcctg
     4501 ggacaaggaa cgccgcgcgc catccgacct gactcgccag gaacgcagtg gcgacaggtg
     4561 ccagcgccac gcccacattg ccgaacacgc cgttgatgcc gattgcgcgc cctggcttgt
     4621 cgccggcagc gtccaccagc atcgcggtgc cgataggatg gtagatcgcc gcgaacagtc
     4681 ccgtcgcagc cagcgtaagt gccagagtga ccggggaact ggcgaatgcg gtcgccacca
     4741 tcgagaggcc tgtgccaacg aagaacaccg tcatcaatgc cggccgcccc acccgctgcg
     4801 cgatccaccc ctgaggcagc gagaacagcc cgtacagcac gaacatcccc gtcgcgagcg
     4861 ccaggatcgg cccatacgcg ccgccgaacc gcccgcctgg cgttgccatg gcgagcacgg
     4921 ctgtcggcag gatcagcagc gaataatgcg tcaccgtgtg agcggcgttg acgaaggtga
     4981 tcaggcgggc atttcgggtc atggacgagc ctccggcatg caatgtagac tgtcgcatgg
     5041 acgacgttca gacatctttt gtctcaaatc ggacaccatt ggaccatgag cggttgcgcc
     5101 cgttcgaaga ggctccccgt cctctcgtcg gctatgcgta cgactacgcg tcggctcatg
     5161 acaccaactg gcaccagcat ccccgcgcgc aattgctcca cgcgacctcc ggcgtgatgc
     5221 gggtggctac acagtcagcg ctgttcatcg tgccaccggg cacgggcctg tggatgcctg
     5281 caaggacgcc gcacgtgacc cggatgccaa caggtctcgc catgcgcgcg ctgttcctgc
     5341 gcgaggacgc ggcgcgtgcc ggcccagaca tggtcaccgt cgtgtccgtg tcgcctttgc
     5401 tccgtgaact gatcctggca gcctgcgaac agccggtgat gtgggatgag gacggccccg
     5461 ttcgtcacgt cgtcgcgctg gcgctgcacg agatcggaca tgccgcgaca aggccgatcg
     5521 ccgtgccggc ctgccgcgat ccgcggctga tgcgtgtcac cgaggcattg ctggcagatc
     5581 ccagcgacac gcgtggcctc gaggcattcg ccgagacggc ggccgcttca tcgcgcacac
     5641 ttgcgcggct gttccgcaaa gaaaccggca tgagctttca ggaatggcga cggcaactcc
     5701 gcttgaccga ggcgctcgca tcattggctc agggtgcgac gccagcgcgc gcggcagcca
     5761 ctgtcggcta tacgagcggt ccggcgtttg gcgctgcgtt caggggagca ttcggcatca
     5821 cgccggggca gtcacgacgg ggctaatgcc aatccgccaa tgacgcccag cacggccgtc
     5881 ccatagcgct gcatcttcga tgctccgacg cccttgatct ggccgagttc ttccacactg
     5941 accggccgca cagctgcgat ctcgcgcagc acggtgtcgt gaaagatgac atagggcgga
     6001 atgcgctgtg ccttggcctc cgcggcacgc caggaacgca gcgcttcgaa cagggtctgg
     6061 acctcggcac cggaatcgtc tttcgccgcc gcggtacgcg ttggccgcaa aacatcccgt
     6121 ggccgcctgc cctcctcacg caacaccacg cgggtctcgc cccgcaggat cgggcgcgcc
     6181 ttctcctgca ccaggaacaa gccgccgtga ccggccgtat caacatccag cgcaccaagc
     6241 gcgataagtt ggcggatcag cccgcgccag tactgcgcgg gatgatcggc acccacgcca
     6301 aacgtctgca accggtcatg cgcatgacgc acaacgcctt cggtcatttc cccacgcagc
     6361 acggcgatga tatggatcgc gccgaacaac tgatcggtgc ggtacacggc ggacagcacc
     6421 ttctgtgcct ctacagtcgc atctacctgg cgcggcggca cggcgcaatt gtcgcaatgt
     6481 ccgcagggct ccggcaggtc ctcgccaaag cacgcaagca aggagcgcgt gcggcaggtc
     6541 accgcctcgg tcagcgcgat catctcttcg agcttggtgc gcatcacacg cttctgcgat
     6601 tctggcgcgg cggattgcgc cagccagtgc cgcgcctgcg cgatgtcctg tccgccatac
     6661 aacaacaatg tctcggaagg atcgccgtcg cggccggcgc gaccgatctg ctggtaatag
     6721 gcctccgggc tgtcgggcat gtcgaggtgc acgacaaaac gcacgtccgg ccgatcgatg
     6781 cccatgccga aggcgatcgt ggcgaccacc acgatcggct cgcctgatcg gaaccgtgtc
     6841 aacgccgcgc gtttgtgttc agcatcaagt ccagcgtgga acgcgaccgc cggcagtccc
     6901 ttatccacca accgcgccgc gacgcgttct gtctttgcgc gcgtcgcaca atagacgatg
     6961 ccgcatgcac cgtgatggcg cgacaacaat tccagaagct gcgccgtctc accaaccttt
     7021 ggctgcgcgc cgatctgcag attcggacga tggaagctcg atatgaacac accagcggtg
     7081 tccatacgca gcgcctgtag aatgtcggcc cgggtgcgcg gatcggctgt tgcggtcaga
     7141 gctacacgcg gcacgccggc gaaacggtcc gccaggcagg caagcgcacg gtattccggg
     7201 cgaaactcgt ggccccattg ggacacgcaa tgcgcctcat cgatcgcgaa aagcgcgagt
     7261 ggcaggcgcg acagccgatc gagcatgcca ttcgccagta accgctcagg cgagacgtac
     7321 aacagatcaa gctcctgctc gatcagatcg cgcgccaccg agcgcgcctc gctcggatca
     7381 agctcggaat gcagcgcgcc agccgcgaca cccagctggc gcagtgccgc cacctgatct
     7441 tccatcaatg cgatcagtgg cgagatcacc actgcagtgc cgcgccggca aagcgccggc
     7501 acctggtagc agatgctctt gccgccgccg gtcggcgcga gcaccagcgc atcgccaccg
     7561 gcgacaacgt gccgtaccac ctcttcctgc tggccgcgaa accccggaaa gccgaatacg
     7621 cggcgcagca catccgccgg ttcgtcgcgg gtctgcacgt gcagcaatga ctgcaacacg
     7681 acttatggcc ggctgatgct gatttccacg cgccggctct cctgcgaggt cagcgcgaac
     7741 tgcaccgagc cgtgacctgt ctgctgaacc tcgcctggag cgactccgtc ggtctgcaac
     7801 tggtcgacca cgacctgcgc gcgcagctca gacagcagga tgttcgcttc tttgctgccg
     7861 gtcggatcag caaatccatt gacatgcgcg acgttgccgg gatgcgattt gacccaggca
     7921 gcggcgttac cgatcaccgt ctgggcattg ttgtcgattg cggccgacca atccccgaaa
     7981 aaaaccacga atttctgcac cggcgggtcg gctgcgcatg tgctggtggt cgccgcaaac
     8041 aacagcagaa gcaataagcc gaggcggcgc atgcccgatt cccttcgtgc gcaggatagc
     8101 cattgtcctg ccgactggtt aacttgccgt caaccgcgat ggcgtgtcgt tcgtgcacga
     8161 aattgcagct caggcggcgg ccgcgatcgc cttgccaccc gacagccgcc agatgcgatc
     8221 cagccgttcc acgccgatca gccggtgcgt gatcaggatc acgctgcgat ccgcggccac
     8281 ctcattcaac gtcgcaagga acgctcgctc tgtctctgca tccaatcccg cgcacggttc
     8341 gtccagtatc aggattggcg cgggtgacag cagagcacgc gccagagcga gtcgccgccc
     8401 ctgaccgccg gagaaacgcg cacccccttc ccccacccag gtatccagtc catctggcag
     8461 cgcgcgcacc atctcgccga tatgcgccgc atccagcgcg gcccatagca ccgcatcatc
     8521 tgcatcgggg ctcgcaagtt tcaggttcgc gcggatcgtg tcgtcgaaca gatgcgtcgc
     8581 ctgcgacagc cagccaaagc gcccccgtac atccgccgca ccaagagttg cgatatcgat
     8641 cccgcccagc gtcaccttgc cctgctgtgg cgcaaccact ttcagagcca acgcggccag
     8701 cgtcgacttc cctgcgccgg acgggccgag cagtgcgacc cgcgcgcctt gcggcacttc
     8761 gagcgacaga ccctcgaata ccggcgcccg atcaggctgc cagcggaagt gcaccgcttc
     8821 gagccgcaag gcaaaaccgc gcggcaaaac gcccggttgc ggcggatcgg gcactgcgac
     8881 tggtgcctct gccgcttgca gcacgcgatg cgccgccgcc gcggcatgcc cggcaagcac
     8941 gccggcacgg ggcaacccgc ccaccgcctc gaaggacgca accacaagaa aagcagcgat
     9001 tacggcggcc gcagggttca tgccggcagc gaccagtacg gcgaggatcg ccatctgacc
     9061 acacagaaac gccccggcgt tggccagcgc cgagcgtgat gccagctcgt gctgcgtcgt
     9121 cagcaaggac gcctctcgtg cttgcacggc ggcaagcata cggccttccg cgccgaaggc
     9181 gcgcacctcg cgcaacccgg tcagcgcatc caatgccgtg atcctcaatg ccgcgatggc
     9241 tccagcgagc cttgccgccg cgcgcgccgc catccttgca gcgaaccatg gcaatacaaa
     9301 cgcggataca gcaaagagaa cgccgatgcc cagcgccagg ccgacgctgt gcagcccgat
     9361 caccacgatc agcaccggca acagcaccac tgcgccggcc agcggcacga ggatgcgcag
     9421 gtaaattccg tccagcgctt cgatatccgt gacgagccgc gccaacacat cgccggcctg
     9481 gcggaacccc agccctcccg cggcgccgcg cgccagattg cgaaagaacc acacccgcag
     9541 atcggccagc gcgcgaaacg ttgcatcatg cgtgctcaca cgttcggcgt agcgcaacac
     9601 gacccgcgtg ctgccgagcc cacgcagcgc gatggatgcc gcaagcacgc cgccggccag
     9661 cagcgcgccg cccaccgcca tcatccctac accggatgcc agtgccgcga gggaaacgaa
     9721 aaggccaatg gcgagccaca ccgcgcggcc gcgccacaag gcaaggatgc gcagcacgtc
     9781 gttcatgcgg caccacgtgc cggcgtcgcg cgtccgtcac gaatgtccag ccgacgcccg
     9841 ccaaatccat gcgctgccga cgagtggctg gccaatatca ccgttcgtcc cagcgccagg
     9901 cggcgcaagc tgtccagcac atctgcctcg gtcgccggat cgagatgtgc ggtgggctca
     9961 tcgagcagca ggagaggcgc attcttcaga aatgcgcgcg caatcgccac gcgttgcgcc
    10021 tggccgcccg agagaccata gcctccctcg cccacctgcg tgtccaaatc gtccggcaaa
    10081 gcagcggcga attcgctcac gctcgcgctt cgggcagcgg cttcgatctc cgcatcggtt
    10141 gcctcaggac gcgcaaagcg gatgttgtcg cggatcgtgc ccgagaacag catgggacgc
    10201 tggccgagcc atgcggtcag gcgcgccaaa gcctgcggca ccagatcagc gatgtcggcg
    10261 ccgttgatcg tgacgcgccc acgatccggc cgcacaaagc cgagcaatat ctcgatcgcc
    10321 gtcgatttgc ccgcgccgga cggaccggcc agcaccagcg tctcacccgc cggcagccgg
    10381 aaggacagcc cgttcagcgc cgggccacgc gacggatccc aggtgagata gacgtcctcg
    10441 aacgccacag tgacgccctg cgccgcaaca gtgcgaatct cccgctgcgg ctgaggctcc
    10501 ggcgcgggcg gcagttcggt cagcgcctca gccgcgccgg cggcatgcag cctgtcctga
    10561 taagcggcgg cgaaggcccg caatggcgcg aaaaactccg gcacgagcag cagcacgaac
    10621 agcgcggcgg ccggctgtgc cagtccgcca gccagcagtt gcgagccata tcggatggcc
    10681 agcacaacga atgccagcgc ggcagccacg tcaagcgcgg cagacgagag aaacgcgacg
    10741 cgcagcaccc gcatcgtccg gcgacgcaac tcgtcggccg ccgtggcaag tgagcgcgcc
    10801 tcggcctcgg cctggccata taggacgatg gtcgcaatgc ctcgcacgcg atccaggaac
    10861 ctcgcttgca ggcgcgccaa cgcaaggaac tggtggcgcg acgctgctgc cgcgccgatc
    10921 ccggcggcgg ccatcgcgac cgggaccatc agaccgcata gaaacaacac cagagcagcg
    10981 aacggatcga ccagcaacgc ggccagcgcc accagaaccg gccccgctat ggccagcatc
    11041 gctgtcggca gccagcgcgc gtacaaaccg tccaatgcct cgatgcgatc gaccacagtc
    11101 gccgtgagcg cgccagtgtg ctgcacgcgc agttgcgagg ggccggcgtg aagcagccgc
    11161 gccagcgcat ccgtgcgcaa ccgccggcgc gcagctgctc cagcgttgaa cgctgccttt
    11221 tcggccacga tcgacacagc ggctcgcacc aacgcgagca tcgcgaaccc gaccatgaat
    11281 ggcgccgtct tgccggcgtg gcgggtcagc gcggcggcga gcaccgaacc ggcgaaccat
    11341 gcctgcccga ttgccagggc gacgccgagc aggttgagca gcatgaccgg ccgcgcggcg
    11401 cgggccccaa tcttctgctc gctgcgcatc caggcgcgcg aggccgcctt gccgtgctcc
    11461 gatgacatcc cgccgcccga ttagcccaca ccgtcggcgg tggacagggc agcaccgtta
    11521 aaagttggca atcgggcgct cggcgcgatc aatacatgac atcaccaccg tttggagaca
    11581 gtgtggcgcc gacgtagaat gcaccgtgcg gacccgccag cagcaatgcg gtcgcggcga
    11641 tctcctccgg gcgaccgaat cgatgaaccg gcagcgcatc gatgaacgcc tgccgatcgg
    11701 ccggctccat cgtggcggtc agatcggtga caatgggccc cggtgccacc gcgttgaccc
    11761 gcaccccctt tggcgccgcc tcacaggcca gcgctcgcgt gaacccgatg atcgcggcct
    11821 tcgccgcaca atagggagca gagttcggtg cgcccttcag acctcgctgt gatgcgacgt
    11881 tgataatgca gccgcgcccg cgcgcggcca ttgccggata gaccgcctgc gccatgaaga
    11941 acatgccctt catgtgcacg tcgaggatcc ggtcgtagtc cgcttcggtg taatcctcga
    12001 acttcttccg gatattgata ccggcgttgt tgaacaggat gtcgatcggc ccgaacgccg
    12061 ccacgacctc ctgcgcgaag ccacggctgg cggcgatatc gaccacgtcg agcgaacgat
    12121 gcatcgcccg ccgcccaagc gcgcggatct cggccgccgt atcgtcagcc tttgcaccat
    12181 cgttcagatg gcagaacgcg atatcggctc cctcggcagc gaacgcgagc gccgttgcgc
    12241 gtccgatgcc acgcgagccc ccggtcacca gagctatctg tccgtccagc tgcattatgc
    12301 ttcctcctcg atccattcgg tccacgcacg gtgtgcgtcg gtccatgcca ctgatcgtcc
    12361 ctcaacggcc agcagcattg tccgtgcgca tgttgcgctg tcggtgcagg ggcaccaagg
    12421 tcacggcatg gatctcgccc gcacgattga cgacttgcat tccgcctgca ccgccttgcg
    12481 ccagcgcttc ggggcattgg ccctggtacc gacgatgggc gcactgcata aagggcatat
    12541 cgcgctggtg cgcgccgcag cggcgagcgg tgcagcggtc gtgacgtcga tcttcgtcaa
    12601 cccgttgcag ttcggcgcca ccgaagacct gacgcgctat ccgcgggatg aggctggtga
    12661 cctcgcgata ttgcgccgtg tcggctgcca cctcgtatgg ctgcccgacg tggcgacgat
    12721 gtatcccgcc gatgccgtca ccacgatcga cctggcaggt ccggccgagc aatgggaagg
    12781 cactgcgcgg ccgggccatt ttcgtggcgt ggcgacagta tgcgccaagc tgttcggcca
    12841 ggtaggagcc ggccgcgcct atttcggcga gaaggactgg cagcagctgc aggtcgtcac
    12901 gcggatggtg gcggatctgc atttgccgtt ggagattgtc ggtgtcccga cagtgcgcga
    12961 tcccgatggg ctggcgctat cttcacgcaa ccggtttctg acgcaacagg aacgtgcgcg
    13021 cgcgccgcgc c
//
LOCUS       DATGEM010000044         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1012769, whole genome shotgun sequence.
ACCESSION   DATGEM010000044 DATGEM010000000
VERSION     DATGEM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012769"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VKQ27_01190"
     CDS             <1..370
                     /locus_tag="VKQ27_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87573.1"
                     /translation="YALGAVAIAVIALVALPYALLLLVSFVAGMLITGSQTGANGTCG
                     KLYPARMRTTGMGWALGIGRLGGIAAPALGGWLLAIGVAPPRIFLSACIFALIAAAAT
                     GLLVFRGNRVPMASIEEVTT"
     gene            complement(378..1409)
                     /locus_tag="VKQ27_01195"
     CDS             complement(378..1409)
                     /locus_tag="VKQ27_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY87574.1"
                     /translation="MWTVAFVLFVAVSGSALAQDAGDYAPQNIGHTYGHLKWLCVLHA
                     LCPVSDKALGVIQGAMANDRSAEYLLGLTLLLGDDLPTDRKAGVIWIARAAELGEPAA
                     ARDIQSRLRNGEAIEVDETKVASALKVQAEAGDAESMRALGPMYIGGRGVARDVAAGI
                     DLLDRAAEKGSTGAELDLSQLYLNGAPGVPKNRPEAMKWLATSAGHGNVKAMVNLGYM
                     AVNGSADAPADLVLGYCWLVRGALVGQPDAQDKLASLFSDGAKDGRGAVIEPDLVQAD
                     LWFRVASRNPYHDNPQIRARIEPQMTTDQLNQAKRLFEAWQPRLVKDITTLTIQLPPP
                     AHGECPPMH"
     gene            1553..>2124
                     /locus_tag="VKQ27_01200"
     CDS             1553..>2124
                     /locus_tag="VKQ27_01200"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HLY87575.1"
                     /translation="MKKLLNDPQLYVEQMLEGLVLAHPQYYERAGDDGRVIKRPGPPR
                     QGKVGIVSGGGSGHLPIFTGYVGKGLLDACAIGEVFSSPSVDQMAAAMRVANAGAGVL
                     RLYGNYGGDCMNFDMAGDIMEMEDVRSTTVLLADDVASAPPAEKQKRRGVAGLVYAFK
                     IAGAAAEAGASLDEVTRIAQKAADSVRSIGA"
BASE COUNT          379 a          719 c          646 g          380 t
ORIGIN      
        1 ctatgcgctc ggcgccgtcg cgattgcggt catcgcattg gtcgcgctgc cctacgcact
       61 gctcctgctg gtgagcttcg tcgccggcat gttgataacc ggcagccaga cgggtgccaa
      121 cggcacgtgc ggcaaactgt atccggcgcg catgcgcacc acgggcatgg gatgggcact
      181 cgggatcggc aggctcggcg gcattgccgc gccggcattg ggaggctggc tgctggctat
      241 cggcgtcgcc ccgccgcgca tcttcctcag cgcctgcatc ttcgcgctga ttgccgctgc
      301 tgcgacggga ctgctggtgt tcaggggcaa tcgcgtgccg atggccagca tcgaagaggt
      361 gacaacctag ccgtgcttca gtgcatcggc ggacattcgc catgcgccgg cggcggcagt
      421 tggatcgtca gcgtcgtgat gtcctttacc aagcgcggct gccacgcctc gaaaagccgc
      481 tttgcctggt tgagttggtc tgtggtcatc tgcggctcga tgcgtgcccg tatctgcgga
      541 ttgtcatgat acggattgcg tgacgcgacc cggaaccaca gatcggcctg caccagatca
      601 ggctcgatga cggcgccacg accgtctttt gccccatcgc tgaacaggct ggccagcttg
      661 tcctgtgcgt caggctgccc taccagcgcg ccccgcacca gccagcagta tcccagcacc
      721 agatcggccg gtgcatcagc cgaaccattc accgccatgt agccaagatt gaccatcgcc
      781 ttaacgttgc catgtcccgc cgacgtggca agccacttca tggcctccgg acgatttttg
      841 ggtacgcccg gtgcaccgtt cagataaagc tgcgacagat cgagttccgc gcctgtcgag
      901 cccttctccg ccgctcggtc aagcagatcg atgcctgctg ccacgtcgcg cgcaacaccg
      961 cggccgccga tgtacatggg tcccagcgcg cgcatcgatt ccgcgtcgcc agcttcggcc
     1021 tgcaccttga gagcactggc gactttcgtc tcgtccacct cgatcgcctc gccattgcgc
     1081 agccgggact ggatgtcacg ggccgcagca ggctcgccaa gctccgcagc ccgcgcgatc
     1141 cagatcacgc cagccttgcg atccgtcggt agatcatcac cgaggagcag cgtcagcccc
     1201 agcagatatt cagccgaccg gtcgttagcc atggcaccct ggatgacgcc aagcgccttg
     1261 tccgataccg ggcaaagagc atgaagcacg caaagccact tcaaatggcc gtacgtgtgc
     1321 ccgatgttct gcggcgcgta gtcgccggcg tcctgcgcca gcgcactgcc ggaaacagcg
     1381 acgaacagca cgaacgctac ggtccacaac cgaaccctca tcatgcgcat ggtctgaacg
     1441 cgcttgcccc cctcgggatt ctgcctaggc taagccaaat ccctgacccc tttcgagttt
     1501 attagtttgc tttgtaccgt ctaccggcca tcggcgccca caggaggaac gcatgaaaaa
     1561 gctgctcaac gatccgcaac tttatgtcga gcagatgctg gaaggcctcg tcctggcgca
     1621 cccgcaatac tacgagcgag ccggcgacga tggccgcgtg atcaaacggc caggcccgcc
     1681 gcgccaaggc aaggtcggca tagtctccgg cggaggatcc ggccatctgc cgatattcac
     1741 cggctacgtc ggcaagggac tgctggacgc ctgcgcgatc ggcgaagtgt tctcctcccc
     1801 atcggtcgac cagatggccg ctgcgatgcg cgtggcgaac gccggggccg gggtgctgcg
     1861 cctctacggc aattacggcg gcgactgcat gaacttcgac atggccggcg acatcatgga
     1921 aatggaggac gtgcgctcca ccaccgtcct gctggccgac gacgtcgcca gcgcgccgcc
     1981 ggcagaaaag cagaaacgcc gcggcgttgc cggcttggtc tatgcgttca aaatagctgg
     2041 cgcggccgca gaagccggtg ccagcctcga cgaggtcacc cgcattgcgc agaaggccgc
     2101 tgactcagtg cgcagcatcg gcgc
//
LOCUS       DATGEM010000045         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_558768, whole genome shotgun sequence.
ACCESSION   DATGEM010000045 DATGEM010000000
VERSION     DATGEM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558768"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="VKQ27_01205"
     CDS             complement(<1..1504)
                     /locus_tag="VKQ27_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013634951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY87576.1"
                     /translation="MILAERHARLTAEANAAAAATNAAVARAEAANAQADLTSSEALI
                     AHLKLAIEKLRRELYGTRSERTARLLDQMELQLEDLEAAATEDELAAKNAAGRTQTVK
                     SFERKRPARKAFPDHLPRERVVIAAPESCPCCGSSKLSRLGEDITETLEVIPRRWKVI
                     QTVREKFSCRACETITQPPAPFHVTPRGFAGPNLLAMILFEKFGQHQPLNRQSERYAR
                     EGIDLSVSTLADQVGACAMVLQPLHALIEAHVLAAERLHGDDTTVPILAKGKTVKGRI
                     WTYVRDDRPFGGRDPPAALYYASRDRRHEHPVRHLRAFAGILQADAYDGYNELYEPSR
                     SPGPITSALCWAHARRQFFELADIAANARRGNNAGAISPVALEAVRRIDVLFDIERGI
                     NGLAADERLRIRKDRSAPVLVELESWLREQRSRMSRSASVAEPIDYMLKRWNRFTRFI
                     EDGSICLTNNAAERALRGFALGRKSWLFAGSERGADRAAAMATLIMTAKLN"
     gene            complement(1600..1947)
                     /gene="tnpB"
                     /locus_tag="VKQ27_01210"
     CDS             complement(1600..1947)
                     /gene="tnpB"
                     /locus_tag="VKQ27_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HLY87577.1"
                     /translation="MIPLPAGVRVWLATGYTDMRKGFPSLALQVQEILQRDPLSGHLF
                     CFRGRSGNLLKVIWHDGQGACLFTKRLERGRFLWPSAADGVVTISSAQLGYLLSGIDW
                     RHPQETWRPTSVG"
     gene            complement(1944..2354)
                     /locus_tag="VKQ27_01215"
     CDS             complement(1944..2354)
                     /locus_tag="VKQ27_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY87578.1"
                     /translation="MDSDKRSTQVERLEVVDTGRRRRWTEDEKFKIVLESLQTPRQIS
                     ATARRYGISRSLLIRWRRAFRAERNDGIEQQHGFVPAMVTPESEAAPAPVGSACRGAI
                     EIEFVAGVRMRITGAVDAATLTAAVAALVDGRPR"
BASE COUNT          481 a          868 c          796 g          458 t
ORIGIN      
        1 cattgagctt tgccgtcatg atcagcgtgg ccatggccgc ggcccggtcg gcaccgcgct
       61 ctgagccggc aaagagccat gacttgcgtc ccagtgcaaa tccgcgcagg gcgcgctcag
      121 ccgcgttgtt cgtgaggcag atgctgccgt cttcgatgaa ccgcgtgaac cgattccagc
      181 gcttgagcat gtagtcgatt ggctcagcaa ccgaggccga acgcgacata cgggaacgct
      241 gctcacgtag ccagctttcc agttcgacca gaacaggcgc actccggtcc ttgcggatac
      301 gcaggcgctc atcggcggca aggccgttga tgccgcgctc gatatcgaac agcacgtcaa
      361 tgcgccggac tgcctccagt gcgaccggcg agatcgcccc ggcgttgttg ccgcgccgcg
      421 cattggcggc gatatcggcc agttcgaaga actgccgcct ggcgtgcgcc cagcaaagcg
      481 ccgacgtgat tggccccggc gagcgggaag gctcgtagag ctcattgtac ccgtcatagg
      541 cgtcggcctg cagaatgccg gcgaaggctc gaagatgccg gaccggatgt tcatgccgcc
      601 ggtcccgcga ggcataatac agcgcggccg gagggtcgcg gcccccaaat ggccggtcgt
      661 cgcgcacata ggtccagatg cgccccttga ccgtcttgcc cttcgccagg atcggaaccg
      721 tggtgtcgtc gccgtgcagc cgctcggccg ccaggacatg agcctcgatc agggcatgga
      781 gcggctgcaa gaccatggcg caggcaccga cctggtcggc cagcgtcgat acgctgagat
      841 caatgccctc gcgggcatag cgctcgctct ggcggttcaa cggctggtgt tgcccgaact
      901 tctcgaacag gatcatggcc agcaggttcg gcccggcaaa gccgcgcggc gtcacatgga
      961 agggcgccgg cggctgcgtg atcgtctcac aagcccggca cgagaacttc tcccgcaccg
     1021 tctggattac cttccatcgc cgcgggatga cctccagcgt ctcggtaatg tcctcaccca
     1081 gcctggacag cttcgacgac ccgcagcagg ggcagctctc aggtgctgcg atcaccacgc
     1141 gctcgcgggg cagatgatcc gggaacgcct tgcgcgccgg ccgcttgcgt tcgaacgatt
     1201 tgaccgtctg ggtccgcccc gccgcattct tggccgccaa ctcgtcctcg gtcgccgccg
     1261 cctccagatc ttcgagttga agctccatct gatccagcag ccgagccgta cgttccgagc
     1321 gcgtcccgta gagctcacgc cgcagtttct cgatcgccag cttgagatgc gcgatcagcg
     1381 cttcgctgct ggtaagatcc gcctgtgcat tggccgcttc ggccctggcg accgcggcat
     1441 tggtcgcggc agctgcagcg ttggcttcag ccgtgaggcg cgcatggcgc tccgcgagga
     1501 tcatcgcatg cgcggcggcg agatcggacg gaagcgattc agcagcgtct ttcatgcgac
     1561 gatggaatca catccgccgt gatccgcaag caaaacctgt tagccgacag aggtcggacg
     1621 ccaggtttcc tgcggatgac gccagtcgat acctgagagc aggtaaccga gctgtgcaga
     1681 agagatcgtc accacgccgt cggctgcgct cggccacagg aagcggcccc gctccagccg
     1741 cttggtaaac aggcatgcgc cctggccgtc gtgccagatc accttcagaa ggttgccgct
     1801 gcgtccacgg aagcaaaaca gatggccgct cagcgggtcg cgctgaagaa tctcctggac
     1861 ctgcaaggcc aacgacggaa agcccttccg catgtcggta tagccagtcg caagccacac
     1921 ccgcacgccg gccggcagcg ggatcatcgt ggccgtccat cgaccagtgc agccacggct
     1981 gctgtcagcg tcgctgcgtc gaccgcgccc gtgatccgca tccgaactcc ggccacaaac
     2041 tcgatctcga tggcgcctct acaggccgag cccaccggtg caggcgccgc ctctgattcc
     2101 ggggtcacca tcgccggcac gaagccgtgc tgttgctcga tgccatcatt acgctcggca
     2161 cgaaacgctc gtcgccatcg gatcagcaac gaccgcgata tgccatatcg ccgcgccgtt
     2221 gccgagatct gccggggcgt ttgtaagctc tccaggacta tcttgaactt ctcatcctcg
     2281 gtccaacgac gacgccggcc cgtatccacc acttcgagcc gttcgacctg ggtactgcgc
     2341 ttatcactgt ccataaggac agttctcaac actgagcctc acctgacaag gcggccttca
     2401 gcgaagggat acgaaagtca cgagggccac cggcttcaaa aacaaaaacg ccgctctttc
     2461 cgaaagggaa gagcggcgtt ttgttcgatc gtgacacgca aagaggaatt gccttgtcct
     2521 tggcaggcct ggcagcgacc tactctccca gggcttaagc catagtacca ttggcgctga
     2581 ggagtttaac ggccgagttc ggg
//
LOCUS       DATGEM010000046         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1354790, whole genome shotgun sequence.
ACCESSION   DATGEM010000046 DATGEM010000000
VERSION     DATGEM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354790"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VKQ27_01220"
     CDS             complement(<1..1121)
                     /locus_tag="VKQ27_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87579.1"
                     /translation="MPDTGVTPPRVASIESRASWMAATLTLAILSISYGAPLLVVVGL
                     KPIQDSLGTDRSVIALAGALVWVGTGLGGIMMGWLADRIGIRATASIGAVMMAAGLAL
                     SSTGSIWALYVGHGLLIGLVGNGAIYAPLVVYVSRWFDRRRGSALAFISSGQYIAGVV
                     WPSVFERGIAQYGWEMTMLAYAAVVVVVILPLTTLCLRPSPEAHAIHAAASSPRKGDR
                     VLGMNPNLAQTLICVAAFFCCIPMAVPNGHLVAFCTDLGIAPAQGAAMLSVLLACAFL
                     SRQFWGMLADRIGGLRAVMVGSACQAVAIGAFLLTQNEIGLFAVSAAFGLGFSGIIPS
                     YVVAIRELFPASEASWRVPTFFFLGMSGMAFGSWLAGAL"
     gene            1202..2422
                     /locus_tag="VKQ27_01225"
     CDS             1202..2422
                     /locus_tag="VKQ27_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY87580.1"
                     /translation="MSDPIDAQPTGPLRGLRVFDLTRVLAGPTCVQMLADLGADVIKI
                     ERPGAGDDTRGFAPPYMPGTKESAYFVGVNRNKRSVTLDMAQPEGQEIARKLIAQCDI
                     LVENFKVGALAKYGLGYEQLHAAYPRLIYCSITGFGQTGPYAPRPGYDSLIQAMGGVM
                     SLTGEPDGLPQKVGVPVADLFAGLYGCIGILAALRHRDATGVGQQIDIGMLDTHAAWL
                     ANQGMNYLATGENPARLGNQHPNIVPYQVFPTADGHIVLSIGNDPTFKRFCEAFALTH
                     LLEDARFATNAARVENRQLVTDTLTPVMQGQNTGWWVEKLEALKIGCGPINKLSEVFA
                     DPHVVARGVVREMAHSSGETVKVIANPVRLSETPVDYRIAAPLLGEHTEQVLGERLGM
                     SRDELAALRTKGVI"
     gene            complement(2514..3320)
                     /locus_tag="VKQ27_01230"
     CDS             complement(2514..3320)
                     /locus_tag="VKQ27_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87581.1"
                     /translation="MEHSSTNEPVASLPDPPQDHPDTTAPSNIEVFIHAVGILLTYGR
                     HLIDTVRHRATAPKFNAIAAGFGTGNLSTILAHLNRGILRAAALERMLLARVAAGKDI
                     DMVQRRTRTPQEDAAPTDDRPAQPASAETPTQKRAPHPPRPAGWNNPELFMPTPEELE
                     RQVRRRPVGRTIFDICLDLAVVPMFCHSAFWNELFELMTFFGGSIDKLMREKARRREA
                     FANEQDRKRDSNWDWLRLKRDDLRQVLGFFIGEPPIDPFDPAAAIATAPP"
     gene            complement(3467..4096)
                     /locus_tag="VKQ27_01235"
     CDS             complement(3467..4096)
                     /locus_tag="VKQ27_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY87582.1"
                     /translation="MPDPTHAYSFRPGTDTARPPLVLLHGSGGDERDLMPLADELAPR
                     AAHLGIRGTVAIDGGYAFFHRFPDRRVDQEDVAARAPLLAGFIAAACGRYSLDQQPIA
                     IGFSNGAIMTAALLLTYPNSLAAAVLFRPLSPFTHDMPHRLDNKPVLIIDGDKDSRRS
                     PGDGSRLAERLVRAGAVVTHHVLPVGHAITGEDRRIAREWLQLNPSVPS"
     gene            4166..4885
                     /locus_tag="VKQ27_01240"
     CDS             4166..4885
                     /locus_tag="VKQ27_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HLY87583.1"
                     /translation="MTWSIVAHDPATGAFAVAVATKAFAVGASCPFARSGVGAVSTQS
                     FTNRYLGPAILDAMARGLAPDAAIESALAGDEGRGIRQVHAVDRHGRTAAWTGQNCVE
                     WCGSVAGPGISVAGNMLAGEPTIGATLAAWQANGALHMPERLMASMEAGEAAGGDRRG
                     RQSAAMVMTTTEDFPDLNLRVDDHVAPLEELRRLLGIWRVERAPGLSAQPSRANPSGF
                     TDLDTIEAAWMLKGLDLRFRR"
BASE COUNT          862 a         1371 c         1915 g          816 t
ORIGIN      
        1 tagagcgcgc cggccagcca gctgccgaac gccatgcccg acatgcccag gaagaagaag
       61 gtcggcacgc gccatgatgc ttcggaggcg gggaagagtt cgcggatcgc cacgacgtag
      121 gacgggatga tgccggagaa gccaagaccg aaagcggcgg aaacggcgaa caggccgatc
      181 tcgttctggg taaggaggaa cgcgccgatt gcgacggcct ggcaggcgga gccaaccatc
      241 accgcgcgca ggccgccgat acgatcagcg agcatgcccc agaactggcg gctgaggaac
      301 gcgcaggcca gcagcaccga gagcatcgcc gcgccctgag ccggggcgat gccgagatcg
      361 gtgcagaagg cgacgagatg gccgttcggc acggccatag ggatgcagca gaagaaggcc
      421 gcgacgcaga tcagcgtctg cgccaggttg gggttcatgc cgagcacacg atcgcctttg
      481 cggggtgagc ttgctgcggc gtgaatggcg tgagcctcgg gtgacgggcg caggcacagc
      541 gtggtcagcg gcaggatcac caccaccacg accgccgcgt aggccagcat cgtcatttcc
      601 cagccgtatt gggcgatgcc gcgttcgaat actgaaggcc agacgacacc ggcgatgtac
      661 tggccggagg agatgaaggc gagcgcggag ccacggcggc ggtcgaacca gcgggagacg
      721 tagacgacga gcggcgcgta gatcgcgccg ttgccgacta ggccgatgag caggccgtgg
      781 ccgacataga gcgcccagat gctaccggtg gaggagaggg cgaggccggc cgccatcatc
      841 acggcgccga tgctggcggt ggcgcggatg ccgatacggt cggcgagcca gcccatcata
      901 atgccgccga ggccggtgcc gacccagacc agtgcgccgg ccagcgcgat caccgaacga
      961 tcggtgccga gcgagtcctg gattggcttc aggccgacca cgacgagcag aggggcgccg
     1021 taggagatcg acaggatggc gagtgtcagg gttgccgcca tccacgaggc acggctctcg
     1081 atactggcca cgcgcggcgg cgtgacgcct gtgtccggca ttgccggcat ctcctcccgg
     1141 ggtataagcg atcgagccgg tatggacctg cgatgacgcc ccggcaagct ggagagacgg
     1201 catgagtgac cccattgacg cgcaaccgac cggaccactt cggggcctgc gcgtgttcga
     1261 tctgacccgg gttctggcgg gaccgacctg cgtgcagatg ctggccgatc tcggcgcgga
     1321 cgtgatcaag atcgagcggc cgggggccgg ggacgacacg cgcgggtttg cgccaccgta
     1381 tatgcccggg acgaaggaga gcgcgtactt cgtcggggtg aaccggaaca agcggtcggt
     1441 gacgctggat atggcgcagc ccgaggggca ggagatcgcg cggaagctga tcgcgcagtg
     1501 cgacatcctg gtggagaact tcaaggtcgg tgcgctggcg aagtacgggc ttgggtatga
     1561 gcagctgcac gcggcgtatc ctcggctgat ctattgctcg atcaccgggt tcggacagac
     1621 ggggccgtat gcgccgcggc cgggatatga ctcgctgatc caggcgatgg gcggggtgat
     1681 gagcctgacc ggcgagccgg acgggctgcc gcagaaggtg ggcgtgccgg tcgcggatct
     1741 gttcgcggga ctctacggct gtattggcat ccttgccgcg ctgcggcatc gcgatgcgac
     1801 cggggtaggg cagcagatcg atatcggcat gctggatacg cacgcggcgt ggctggcgaa
     1861 ccaggggatg aactacctgg cgaccggcga gaacccggcc cggctgggga accagcatcc
     1921 gaacatcgtg ccgtaccagg tgtttccgac ggcggatggg cacatcgtgc tgtcgatcgg
     1981 caatgatccg accttcaagc ggttctgcga ggcgttcgcg ctgacgcatc tgctggagga
     2041 tgcacggttc gccaccaatg cggcgcgggt ggagaaccgg cagctggtga ccgacacgct
     2101 gacgccggtg atgcaggggc aaaacaccgg ttggtgggtg gagaagctcg aggcgctgaa
     2161 gatcggctgc ggaccgatca acaagctgtc cgaggtgttc gctgatccgc acgtggtcgc
     2221 gcgcggcgtg gtgagggaga tggcgcacag ctcgggcgag acggtgaagg tgatcgccaa
     2281 tccggtgcgg ctttcggaga cgccggtgga ttaccggatc gccgcgccgc tgctggggga
     2341 gcacaccgaa caggtgcttg gcgagcggct tgggatgagc cgcgacgaac tggcggcgtt
     2401 gcggacgaag ggcgtgatct gaagggcaag gaaaatggaa ccgcagacca cgcagatggc
     2461 ggccggcgag ggcgggttgg acctgcgctg gaaggcgctt ttgccgacgt gcgtcagggc
     2521 ggggcggtgg cgatggcggc ggccgggtcg aacgggtcga tcggtggctc gccgatgaag
     2581 aagccgagga cctgacgaag gtcatctcgt ttcaggcgta accaatccca gttgctgtcg
     2641 cgcttgcggt cctgctcgtt ggcgaatgct tcgcgacggc gggctttttc gcgcatcagc
     2701 ttgtcgatgc tgccgccgaa aaaggtcatg agctcgaaca gttcgttcca gaaggcgctg
     2761 tggcagaaca ttgggacgac ggcgagatcg agacagatgt cgaagatggt gcggccgacg
     2821 ggacggcggc gtacctggcg ttcaagctcc tccggtgtgg gcatgaagag ttcgggattg
     2881 ttccagccgg cggggcgggg tgggtgaggt gcgcgcttct gcgtcggggt ttcggcagag
     2941 gcgggttgcg cgggccggtc gtcggtcggg gcggcatctt cctgcggcgt gcgggtgcgg
     3001 cgctggacca tgtcgatgtc tttgccggct gcgacgcggg cgagcagcat gcgctcgagt
     3061 gcggcggcgc ggaggatgcc ccggttcagg tgagcgagaa tggtggagag gttgccggtt
     3121 ccgaagccgg cggcgatggc gttgaatttc ggggcggtgg cgcggtggcg gacggtgtcg
     3181 atcaggtggc ggccgtaggt gagcaggatg ccaacggcgt ggatgaagac ctcgatgttg
     3241 ctgggcgcag tggtgtcggg gtgatcctgc gggggatcgg gaagggaggc gaccggttcg
     3301 ttggtactgc tgtgctccat tgggtattta cgctaacgga cctgggggta gttgagcaag
     3361 gaatattttc atggatctcg tcagtttgtc ggggcgacgt gccgcgacgg atggccgggt
     3421 cgagccccgg ccatgacggg gggacgggga gccggaggag gggcgatcag gatgggacgg
     3481 aggggttcag ctgcagccac tcgcgggcta ttcgcctgtc ctcgccggtg atcgcgtggc
     3541 cgaccggcag cacgtgatgc gttactaccg cgccggcgcg gaccagccgc tctgccagtc
     3601 gcgagccgtc gcctggtgat ctgcggctgt ccttgtcgcc gtcaatgatc agcaccggct
     3661 tgttgtcgag gcggtgtggc atatcgtggg tgaatgggga gaggggacga aacaggactg
     3721 ctgccgccaa cgaattgggg taggtcagca gcagggccgc cgtcatgatt gctccgttgg
     3781 aaaagccgat tgctatgggc tgttggtcga ggctgtagcg gccgcaggcg gctgcgatga
     3841 agccggccaa caagggtgct ctggcggcga catcctcctg gtccacgcgg cgatccggaa
     3901 aacgatggaa gaaggcgtag ccgccgtcta tcgcaaccgt gccacgaatg ccgagatgtg
     3961 ccgcccttgg tgccagttca tcagccaacg gcatgagatc gcgttcatcg ccgccggaac
     4021 catggagcag aacgagtggc ggcctggcgg tatcagtgcc aggacggaag ctgtaagcat
     4081 gtgtcgggtc aggcatggca gcaagccaac cggttttgtg cgatgtggat tgagcttata
     4141 gccccttgtt cccggtggag gtccaatgac ctggtccatt gttgcccatg atcccgcaac
     4201 cggtgcattc gcggttgcgg ttgcgaccaa ggcgttcgca gtgggggcgt cgtgtccgtt
     4261 cgcgcggtcg ggtgttgggg cggtatccac ccagtcgttc accaatcgct atcttggtcc
     4321 cgccatcctg gatgcaatgg cgcgaggcct ggcaccggac gcggcgatcg agagtgcgtt
     4381 ggctggtgat gaaggccgtg ggatccgcca ggtgcacgcg gtggatcggc acgggcgcac
     4441 ggcggcctgg accgggcaga actgtgtcga atggtgtggg agcgtggccg ggcccggcat
     4501 cagcgtggcc ggcaatatgc tggcggggga gccgacgatc ggggcgacgc tggctgcttg
     4561 gcaggccaat ggtgcgttgc atatgccgga acggctgatg gcgtcgatgg aggcgggcga
     4621 ggcggcaggc ggcgaccggc gagggcggca atcggcggcc atggtgatga ccacgaccga
     4681 ggatttcccg gacctgaacc tgcgcgtcga tgatcatgtg gcgccgctcg aggagcttcg
     4741 gcggctgttg gggatctggc gcgtggagcg tgcgccgggc ctgtcggcgc agccgtcccg
     4801 ggccaatccg tcagggttca ccgatcttga tacgatcgaa gcggcctgga tgctgaaggg
     4861 gctggatttg cggttccgcc gctagcgggg ggcgtcaacc tctggcagat tgaatattgc
     4921 gtgaccggcg gcggaggccc gcctaatcat gcggttagac actg
//
LOCUS       DATGEM010000047         6651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_792608, whole genome shotgun sequence.
ACCESSION   DATGEM010000047 DATGEM010000000
VERSION     DATGEM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792608"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1197
                     /gene="dnaJ"
                     /locus_tag="VKQ27_01245"
     CDS             49..1197
                     /gene="dnaJ"
                     /locus_tag="VKQ27_01245"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLY87584.1"
                     /translation="MAKQDFYATLGVARDAGTEELKKAYRKLAMQFHPDRNPGDKQAE
                     AKFKEVNEAYDILKDDQKRAAYDRYGHAAFEQGGQGGFGGGFDFQAAGGMGDIFEQMF
                     GEFMGGRRGGGAGRTRAGNDIRAAVEIELTEAFAGTKANVRVPTRVVCDACSGTGSED
                     KNRAADTCSTCKGTGKLRTQQGFFLVERTCPTCGGAGRTIRNPCKVCHGVGTVGRERT
                     LQVQIPPGVEDGTRIRLAGEGEAGGQGAPPGDLYVHVAIRPHAIFQRDGSNILMRVPL
                     RMTQAALGGDVEVPSIDSSKAKVKIPPGTQTGDQFRLRGKGFSVLRSAVRGDMYIQVS
                     VETPQHLTRRQRELLEEFELEAEGHGKGSPESEGFFSKVKEFFEGGRG"
     gene            1262..3439
                     /locus_tag="VKQ27_01250"
     CDS             1262..3439
                     /locus_tag="VKQ27_01250"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLY87585.1"
                     /translation="MPTSPRLARIIGGTRLLAHLLQHAVRPRPAVSLQTRLAMVPLDK
                     APLDQPVTIHWDDHHIPFIDAATDHDAATALGIVHAHLRLGQLEVLRRLSHGRLSEMI
                     GAAGAELDRQLRALDPGRAVPGMLAMMPAATREWLDAFARGINHNLMRTPRLPPEFSL
                     FALQRETWTPADVLTVGRLMSADVNWLVWLRLLQFRSDPDWPALWRKLAEADSLSFDS
                     AELLGAPIRSGSNSLVIGPARTQSGAPMIASDPHLPLVLPNPWLIAALKSPSYNMVGL
                     MLPGLPFVALGRNSWIAWGGTSLHAASSDVVAVAASDIEHAEQQIKVRWGKPRTVQVP
                     NSPWGPVMSDTLALRWMGHRPSDEVTAMLAVNRARNWDEYRAALDGFSVPGQNMLYAD
                     TEGHIGKLMAVHLPRRNEFPSDDLVLSPDRQQGWDDPVTGSELAHEFDPVHGFIASAN
                     ERPPECGVFIGHQFSRPDRRRRLDHMLSATNNATVAQAIDIQRDVHWEQGHAQCRQLL
                     RWMPPVPAGRQQRFINLLASWDGNYNVNSRGALAFELLSNALARLLVTKRRLAAYSLA
                     WGTRRLVWSEIVAAPPDRRQRALRIAVRQAARTLRPRASWGSQHRLRLGHPLTALPVI
                     GRRWRFTDLPVGGGNDTLFKTAHGLTDRIHGCGYGSTARHISDLSDPDRNLFALAGGQ
                     DGWPGSTTFIDQVHSFLCSDYITVPLRAETARATFPHRMDLTP"
     gene            3436..4983
                     /locus_tag="VKQ27_01255"
     CDS             3436..4983
                     /locus_tag="VKQ27_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HLY87586.1"
                     /translation="MRRVPFDATFLLRQYARRRSAKLAALDSVAEQQHQLLRLVRRAG
                     ATRFGHDHHFAGVRSVDEFQAHVPLRRYEDMWQTYWQPGFPRLVDCTWPGTIPFFALT
                     SGTTSGTTKYIPCSADMNRSNARAALDILVHHLANRPGSRVLGGKSFMLGGSTDLTEL
                     APRIRAGDLSGIAAQQTPWWARPYTFPSRDLALLTDWEEKVERLARACRDEDIRVISG
                     TPSWLLIFFHYLPQGLLHAFPNLELLVHGGVNFAAYQDQFAELLRGGRAELREVYPAS
                     EGFIAIADRGSGDGMRLQVDNGIFYEFVPVGDIDADRPARHWLANVATGVDYAIAVST
                     NAGLWSYLVGDIVRFVDLAPPRLLVTGRLSYFLSAFGEHLTGEDVDAAISHAATATCA
                     NVTDYAVGAVFPDASDSRGGHRYIIEFATPPSDAFRFAQMLDAELCRRNDDYRAHRSA
                     GFGLRDPGILPVRSGTFAAWMKQRGQLGGQHKVPRVITNAALFESLCRFAASVQYERD
                     EGGSHGT"
     gene            4973..5740
                     /gene="dapB"
                     /locus_tag="VKQ27_01260"
     CDS             4973..5740
                     /gene="dapB"
                     /locus_tag="VKQ27_01260"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLY87587.1"
                     /translation="MAPNIGIAGVTGRMGQLLVEEVRAAGAEVVGGVSRNKSAPQGIA
                     FFPDISALAASSDVVIDFTNAATAQPTAAAMAKSGKAWVLGTSGLSESDEAAVAEAAH
                     RIPIVYASNFSAGVNLVLALAERMGAALPADSYDAEIVEMHHRQKVDAPSGTAVGMGR
                     AVAKGRDVALSDVMESGRHGHTGARKTGAIGFAALRGGQVVGEHTLIFASGTEHIALT
                     HRAFDRRAFATGAVRAALWAAGRKPGLYSMMDVLGMA"
     gene            5737..6327
                     /locus_tag="VKQ27_01265"
     CDS             5737..6327
                     /locus_tag="VKQ27_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87588.1"
                     /translation="MNEGAARFAARYPVVWHVIEADGAGDWLVETGLMPTAELMRMAG
                     IIDDGANRNDFRTLSLGRGRIAVIRPQLMPDDRLRPTLDGPFRDLPNTWRRHINSHVF
                     FWADPRRRDAFLRACQRFRPSAATPVVLEFDTASLLAGHAACAFWSRINTGSTVRGGA
                     RTRRNENTLQSVRSYRSGPVAELAVRGQVTVTRNRP"
     gene            6388..>6651
                     /locus_tag="VKQ27_01270"
     CDS             6388..>6651
                     /locus_tag="VKQ27_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87589.1"
                     /translation="MRDTGEFLFTSESVSEGHPDKVADRLSDTVLDAYLSADPYSRVA
                     CETLVTTNRVVLAGETRGPDTITPEYLTHLARMAIHDIGYDQDG"
BASE COUNT         1167 a         2363 c         1991 g         1130 t
ORIGIN      
        1 cctggaaggc gggcgcgatt ccattcggtg ccagagagaa gggctgagat ggcgaagcag
       61 gatttctatg ccacgcttgg cgtggcccgc gatgcaggta ccgaagagct caagaaggcg
      121 tatcgcaagc tcgccatgca gttccacccc gatcgcaatc cgggcgacaa gcaagcggaa
      181 gccaaattca aggaagtcaa cgaagcttac gacattctca aggacgacca aaagcgcgct
      241 gcctatgacc gttacggcca cgcggcgttc gagcaaggcg gacagggcgg cttcggcggt
      301 ggcttcgatt tccaggccgc tggcggcatg ggcgacatct tcgagcagat gttcggcgaa
      361 ttcatgggcg gccgccgcgg cggcggtgcc ggccgcaccc gcgccggcaa cgacatccgc
      421 gctgccgtcg agatcgagct caccgaagcc ttcgcgggca ccaaggcgaa cgtgcgggtt
      481 ccgacacgcg tggtgtgcga tgcctgctcc ggaaccggct ccgaggacaa gaaccgcgcc
      541 gccgatacct gctccacctg caagggcacc ggcaagctgc gcacgcaaca gggcttcttc
      601 ctggtcgagc gcacctgtcc cacctgcggc ggcgctggtc gcaccatccg caatccctgc
      661 aaggtctgcc atggtgtcgg tactgtcggc cgcgagcgga cgctgcaggt gcagatccca
      721 cccggtgtgg aggacggcac ccgcatccgc ctggccggcg aaggcgaggc tggcggccag
      781 ggtgccccgc ccggcgatct gtatgtgcac gtggcgatac ggccgcacgc gatcttccag
      841 cgcgacggct ccaacatcct gatgcgcgtg ccgctgcgca tgacccaggc cgcgttgggc
      901 ggcgacgtgg aagtgccctc gatcgacagc agcaaggcca aggtgaagat cccgcccggc
      961 acgcagaccg gcgaccagtt ccgcctgcgc ggcaagggat tctccgtgct gcgcagcgcg
     1021 gtgcgtggcg acatgtatat acaagtctcg gtcgagacgc cgcagcattt gacgcgccgc
     1081 cagcgcgagc tgcttgagga attcgaattg gaagcggaag gccacggcaa aggcagcccg
     1141 gagagcgaag gctttttctc caaggtcaaa gaattcttcg agggcggccg cggctagcct
     1201 cgacaaacca tccctctccg ccccttgggg gcggagaggt taggagaggt gggggccgcc
     1261 cgtgcccaca tcaccacgcc tcgcgcggat cattggcggc acacgcctgc tggcccactt
     1321 gctccagcac gccgtccgcc cccgccctgc cgtaagcctg cagacccgtc tcgcgatggt
     1381 gccgctagac aaggcgccac tcgatcagcc agtgacgatc cactgggacg atcaccacat
     1441 ccccttcatc gatgcggcca ccgaccatga cgccgcaacg gcattaggaa tcgtccatgc
     1501 ccatctccgc cttgggcagc tcgaggtact gcgccgcctc tcccacggcc gcctctccga
     1561 gatgatcggt gcggcgggag ccgaactcga tcgtcagctt cgcgcgcttg atccaggccg
     1621 cgccgtcccc ggcatgctgg caatgatgcc cgcagccacg cgcgaatggc tggatgcctt
     1681 cgcgcgcggc atcaatcata atctgatgcg cacgccgcgc ctgcccccgg aattttcgct
     1741 attcgctttg caacgcgaaa cctggacccc cgccgacgtg ctgaccgtcg gccgcctgat
     1801 gagcgcggac gtcaactggc tggtctggct gcgactgctg caatttcgca gcgatccgga
     1861 ctggcccgcg ctgtggcgca aactcgccga ggccgactcc ctgtcgttcg attctgccga
     1921 acttctcggt gcgccaatcc gctccggcag caactcgctg gtgattggcc cagcacgcac
     1981 gcaaagcggc gcaccaatga tcgcaagcga tccgcatctg ccattggtgc tacccaatcc
     2041 gtggctgatc gctgcgttga agtcgccgtc ctacaacatg gttggcctga tgctgcccgg
     2101 cctgcctttc gtggcactcg ggcgcaattc gtggatcgcc tggggcggca ccagtctgca
     2161 cgcggccagc agcgatgtgg tcgcagttgc cgcctccgac atcgaacacg ctgaacagca
     2221 gatcaaggtg cgatggggca agccccgaac agtgcaggtg cccaactcgc cctggggtcc
     2281 agtaatgagc gataccctgg ccttgcgctg gatgggccac cggccaagcg acgaggtgac
     2341 agccatgctt gccgtcaatc gcgctcgcaa ctgggatgag taccgcgccg cgctcgatgg
     2401 cttctccgtg cccggccaga acatgctcta cgcggacacc gaagggcata tcggcaagct
     2461 gatggccgtc cacctcccgc gccgcaatga gttcccttca gacgatttgg ttttgtcgcc
     2521 ggaccgccag caaggctggg acgacccggt caccggcagc gaactggcac acgagttcga
     2581 tcccgtgcat ggcttcatcg cttcagccaa tgagcgcccc cccgaatgcg gtgtcttcat
     2641 cggccaccag ttctcgcgcc ccgatcgcag gcgccggctc gaccacatgc tgtcggccac
     2701 caacaacgcc acggtggcgc aagcgatcga tatccagcgt gacgtccact gggagcaagg
     2761 ccacgcgcaa tgcaggcaac tgctgcgctg gatgccaccg gtgcctgccg ggcgtcagca
     2821 acgcttcatc aacctgctcg catcctggga cggcaactac aacgtcaact cccgtggcgc
     2881 actcgccttc gaactcctga gcaatgcgct cgcacgcctg ctcgtaacca agcgccgtct
     2941 cgcagcctac agcctcgcct ggggaacacg ccgcctggtc tggagcgaga tcgtcgcggc
     3001 cccaccagac cgtcggcagc gggctttgcg gatcgcagtg cgtcaggccg cgcgaaccct
     3061 gcggccgcgc gccagctggg gcagccagca tcgcctgcgt ctcggtcatc cgctgaccgc
     3121 gcttcccgtt atcgggcggc ggtggcgctt caccgatctt cccgtcggcg gcggcaacga
     3181 cacgctgttc aagaccgcgc acggcctgac cgaccgtatc cacgggtgtg gctacggctc
     3241 taccgcccgc cacatctcgg atctgtccga cccggatcgc aacctgttcg cgctcgccgg
     3301 cggccaggat ggctggcccg gcagcaccac cttcatcgac caggtccact cattcctgtg
     3361 cagcgactac atcaccgtac cactccgcgc cgaaacagcg cgcgcgacat tcccgcatcg
     3421 catggacctg acgccgtgag gcgcgtgcct ttcgatgcga ccttcctgct acgccaatac
     3481 gcccgccgac gttcggccaa actcgccgca ctggattccg tcgccgaaca gcagcaccag
     3541 ctgctgcgat tggtgcgccg agccggggcg acacgcttcg gccacgatca ccactttgcc
     3601 ggcgttcgca gtgtcgatga attccaggcc cacgtccctc tgcgacgcta tgaggacatg
     3661 tggcagacct actggcaacc aggctttcca aggctcgtcg attgcacctg gccaggcacc
     3721 atcccgttct tcgccctgac ctccggcacg acaagtggca cgaccaaata catcccatgc
     3781 tcagccgata tgaatcgctc gaacgcacgc gccgcactcg acattctggt acaccatctc
     3841 gccaaccgcc ccggcagccg cgtgctgggc ggcaagagct tcatgctcgg cggcagcact
     3901 gatctcaccg agcttgcacc tcgtatccgc gccggcgatc tcagcggtat cgccgcacag
     3961 cagacgccat ggtgggcgcg accctacaca tttccgtcac gcgacctcgc cctgctcacc
     4021 gactgggagg agaaagtcga acgattggca cgtgcatgcc gcgacgaaga catccgcgtc
     4081 atcagcggca cgccaagttg gctgctcatc ttcttccact acctgccgca gggcctgctc
     4141 cacgcgtttc ccaacctcga attgctcgtt catggtggtg tgaacttcgc ggcctaccag
     4201 gatcagttcg ccgaactact gcgcggtggc agggccgaat tacgcgaagt ctatcccgcc
     4261 agtgaaggtt tcatcgccat cgccgacagg ggatcgggcg acggcatgcg gctgcaagtg
     4321 gacaacggca tcttctacga attcgttccg gtcggcgata ttgatgccga ccggccagca
     4381 cgccattggc tcgccaatgt ggcaacaggt gtggactacg cgatcgctgt ctccacgaat
     4441 gcaggcctgt ggtcctatct cgtcggcgac atcgttcgtt tcgtcgatct cgcgccgcca
     4501 cgtctgctgg tcaccgggcg cctctcctat ttcctctccg ctttcggcga acatctgacc
     4561 ggcgaggacg ttgacgctgc gatcagtcat gccgccacag caacctgcgc gaacgtcacc
     4621 gattatgccg tcggcgcggt gttccccgat gcatcagact cgcgcggtgg ccatcgctac
     4681 atcatcgaat tcgccacacc cccgtccgac gcatttcggt ttgcgcaaat gctagacgcc
     4741 gaactgtgcc gccggaacga tgactaccgc gcccatcgtt cggccggctt cggcttgcgc
     4801 gacccgggta ttctccctgt ccgctccggc acttttgccg cctggatgaa gcagcgcgga
     4861 cagctgggcg gacagcacaa agtccctcgc gtgatcacca acgcggcact tttcgagtcg
     4921 ctatgccgat ttgccgcgag cgttcagtat gaacgtgatg agggagggag ccatggcacc
     4981 taatatcggc atcgcaggcg tcaccggacg aatgggccaa ctgctggtgg aggaagtccg
     5041 cgctgccggt gccgaagtcg tcggcggtgt cagccgcaac aaatcggcgc cccagggcat
     5101 cgcctttttc cccgacatct ccgccctcgc agcgtcatcc gacgtcgtga tcgacttcac
     5161 caacgccgca actgcgcagc ccaccgccgc cgccatggcg aaatcgggca aggcctgggt
     5221 gctcggcacc tccggcctct ccgagtccga cgaagccgcc gtcgccgaag ctgcccatcg
     5281 catccccatc gtctacgcct ccaacttctc cgccggcgtt aacctggtgc tggcgctcgc
     5341 cgaacgcatg ggcgccgccc tccccgccga ctcctacgac gccgagatcg tggaaatgca
     5401 ccaccgccag aaggtggacg ccccctccgg caccgccgtc ggcatgggcc gagccgtcgc
     5461 caagggccgc gacgtcgccc tgtccgacgt gatggagagc ggtcgccacg gccacaccgg
     5521 cgcgcgcaag acgggcgcca tcggctttgc cgcactgcgc ggcggccagg tcgtgggcga
     5581 gcacacgctg atcttcgctt ccggcactga gcacatcgcg ctcacccatc gcgccttcga
     5641 ccgccgcgcc ttcgccaccg gtgccgttcg cgcggccctc tgggcggcag gccggaagcc
     5701 tggcctctac tccatgatgg atgtgctggg gatggcttga acgagggtgc cgctcgattc
     5761 gccgcgcgct accccgtcgt ctggcacgtt atcgaagcag atggcgcagg tgactggctt
     5821 gtcgagacgg gcttgatgcc caccgccgag ctgatgcgaa tggccggcat catcgacgat
     5881 ggcgccaacc ggaatgactt ccgcacgctg tcgctcggtc gagggcgcat agccgtcatc
     5941 cggccgcaac tcatgccaga cgaccggttg cggcccacgc tggacggccc cttccgcgac
     6001 ctgccgaaca catggcgccg gcacatcaac tcacacgtgt tcttctgggc cgacccccgc
     6061 cgccgcgatg cgtttctgcg cgcctgccag cgtttccgcc cgtcggccgc aacacccgtg
     6121 gtgcttgaat tcgacacagc cagcctgctt gctggccatg ctgcctgcgc gttctggtcg
     6181 cgcataaaca cgggctcaac agtgcggggc ggcgcccgca cccgccgtaa cgagaacacg
     6241 ctgcaatcgg tgcgcagcta ccgctctggc ccggtcgcgg aactagcggt gcgaggacag
     6301 gtgacagtca ctcgaaaccg cccttgaccc ccccaaacgt ttccgccaca ccccggcgat
     6361 cctcccgcga caagagagaa accattcatg cgcgatacag gcgagttcct gttcacctcc
     6421 gagtcggtct ccgagggcca ccccgacaaa gtcgccgatc ggctcagcga tacggtactg
     6481 gacgcttatc tctcggccga cccatattcg cgcgtcgcct gtgagacttt ggtcaccacc
     6541 aaccgcgtgg ttcttgctgg cgagacccgt ggcccggata ccatcacccc ggaatacctc
     6601 acgcacctcg cgcgcatggc gatccacgat atcggctacg accaggacgg c
//
LOCUS       DATGEM010000048         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_781767, whole genome shotgun sequence.
ACCESSION   DATGEM010000048 DATGEM010000000
VERSION     DATGEM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781767"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VKQ27_01275"
     CDS             complement(<1..498)
                     /locus_tag="VKQ27_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HLY87590.1"
                     /translation="MKFDIAHPDVRRAVEAALAEDVGAGDITTDATVPADLQAEARFV
                     ARENMTVAGVELLSLLFDQPVFDVSSGSRLQRGDEIALIRGPARLLLTRERVALNFLQ
                     RLSGIATLAARFVKAVEGTGVRIQDTRKTTPGLRVLEKMAAAAGGVANHRMGLYDAVL
                     IKNNHI"
     gene            complement(495..>2620)
                     /locus_tag="VKQ27_01280"
     CDS             complement(495..>2620)
                     /locus_tag="VKQ27_01280"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLY87591.1"
                     /translation="AGVSCDWSRERFTLDPGLSRAVREAFVRLYEKGLIYQGDYIVNW
                     CPRCQTALSDLEVEHEDTEGSLWHIRYPVIGSDESIVVATTRPETMLGDTAVALNPRD
                     KRAAALHAKAVRLPLMDREIPIVLDEMADPEFGSGAVKITPAHDPNDFEAGKRHDLPS
                     IQVIGEDAKMTAAAGRFAGLDRFEARKRVVEELKALGLLEKIEPYKLSVGKCHRCKTV
                     VEPLVSKQWWMRMKPLAKPAIEAVEDGRITFVPANWAKTYFEWMYNIRDWCVSRQLWW
                     GHRIPAWHCGDCKAVTVARETPTACSKCGSKNLKQDPDVLDTWFSSGLWPFSTLGWPD
                     DTADLRTFYPTTLMVTGFDIIFFWVARMIMLGMEMMGDVPFRQIHIHGLVRDAERQKM
                     SKTKGNVIDPLVLNEKYGTDAVRFGLLVAAAPGNDIALSEEVIARGRNFANKIWNASR
                     LLFSKPQSEPVRESLADRWIGHRLNWCAEQMNRAFKDHRYHEAADAIWTFFWDEFCDW
                     YLEFKKGESDWGYAYEVHEKALLLLHPLMPFLTEELWHRRGHTTSIALELYPQFNSSN
                     SDALAANVMQVIQAIVTEIRQGRASNNVAKGQILTAELSAQGSAYARALANKEAIERL
                     GSVTLTVKEGAESPYALKLSIPVDRVRLEGENEQLEKVILGTSGQLGNQDFITKAPAK
                     VVDAMHAKLAEYRARLAKNKAALGE"
BASE COUNT          476 a          814 c          815 g          515 t
ORIGIN      
        1 aatgtgattg ttcttgatca gcacggcgtc gtagagtccc atgcgatgat tcgccacgcc
       61 acccgcggct gcggccattt tctcgagcac acggaggccg ggcgtagtct tgcgcgtatc
      121 ttgaatgcgg acgccggtgc cttcaacggc cttgacgaag cgcgcggcca gcgtggcgat
      181 gccggagagg cgctggagaa aattgagcgc gacgcgctcg cgcgtgagca gcaggcgcgc
      241 gggaccgcgg atgagggcaa tttcgtcgcc acgctgaagg cggcttccgc tcgacacgtc
      301 gaacacaggt tgatcgaaaa gcagcgagag cagctcgacg ccggcgacgg tcatgttttc
      361 tcgagcgacg aaccgcgctt ccgcttgcag gtcagcaggc acagttgcgt cggttgtgat
      421 atcgcccgcg ccgacatcct cggccagcgc ggcctcgacc gcgcggcgaa cgtcgggatg
      481 agcgatgtcg aatttcattc gccgagcgcc gctttgttct tggcgagccg cgcccggtac
      541 tcggccaact tcgcgtgcat ggcatcgacc accttggcgg gcgccttggt gatgaagtcc
      601 tggttcccta gctggccgct ggtgccgaga atcacctttt cgagttgctc gttttcacct
      661 tccagccgga ctcggtcgac gggaatactc agcttcagag catacggaga ctcggcccct
      721 tccttcaccg tgagcgtcac acttccaaga cgctcaatag cttctttgtt cgccagcgct
      781 ctcgcgtacg cggagccctg tgcgctgagc tctgcggtga ggatctgacc cttagcgaca
      841 ttgttagagg cccggccttg gcggatctcg gtcacgatag cctgaatcac ttgcatgacg
      901 ttagcagcca gggcatcact gttactcgaa ttgaattgag gatacaactc aagcgcaatg
      961 gaagtcgtgt gtcccctccg atgccacagt tcctcagtga ggaatggcat cagaggatga
     1021 agcagcagaa gtgccttctc gtgcacttcg taggcgtagc cccagtcgga ctcgcccttc
     1081 ttgaattcga gataccagtc gcagaactcg tcccagaaga acgtccagat agcgtcagcg
     1141 gcctcatgat aacggtggtc tttgaatgca cgattcatct gctcagcgca ccaattgagg
     1201 cgatgtccga tccagcgatc cgccaatgac tcgcgcaccg gttcactttg cggtttgctg
     1261 aacaacagcc gcgaggcgtt ccagatcttg ttggcgaagt tgcggccgcg agcgatgacc
     1321 tcttcgctca gcgcgatgtc gttgccgggg gcggcggcca ccagcaggcc gaagcgcacc
     1381 gcgtcggtcc catatttttc gttgagcacc agagggtcga tcacgttgcc cttggtcttg
     1441 gacatcttct ggcgctcggc gtcgcgcacc aggccgtgga tgtgaatctg gcggaaggga
     1501 acgtcaccca tcatctccat cccgagcatg atcatgcgcg ccacccagaa gaagatgatg
     1561 tcgaagccgg tgaccatcag cgtggtgggg taaaacgtgc gcaggtcggc ggtatcgtcc
     1621 ggccagccga gcgtcgaaaa gggccacagg cccgaactga accaggtgtc gaggacatcg
     1681 gggtcctgct tgagattttt cgagccgcat ttcgagcacg ccgtcggcgt ctcgcgcgcc
     1741 acggtgaccg ccttgcaatc gccgcagtgc cacgccggaa tgcgatggcc ccaccacaac
     1801 tggcgcgaaa cgcaccagtc gcggatgttg tacatccact cgaagtaggt cttggcccaa
     1861 ttggcgggaa cgaaggtgat gcggccgtcc tcgacggcct cgatcgcggg cttggcgagg
     1921 ggcttcatcc gcatccacca ttgcttggag accagcggct cgaccacggt cttgcagcga
     1981 tgacatttgc cgacgctgag tttgtacggc tcgatcttct cgagcagccc cagcgccttc
     2041 aattcctcga cgacgcgttt gcgcgcctcg aaccggtcca gacccgcgaa acgcccggcg
     2101 gcggcggtca ttttcgcgtc ttcgccgatc acctgaatgc tgggcaggtc gtggcgcttg
     2161 ccggcctcaa aatcgttggg gtcgtgggcg ggcgtgatct tgacggcgcc cgagccgaat
     2221 tcgggatcgg ccatttcgtc gaggacaatc gggatctcgc gatccatcag cggcagccgg
     2281 acggctttgg cgtgcagcgc cgccgcgcgc ttgtccctgg gattgagcgc cacagccgtg
     2341 tcgccgagca tcgtctcggg ccgcgtggtg gcgaccacga tggactcatc gctcccgatc
     2401 acgggataac ggatgtgcca cagactgccc tcggtgtcct cgtgctcgac ttcgagatcg
     2461 gacagcgccg tctggcagcg cgggcaccag ttgacgatgt aatcgccctg gtagatgaga
     2521 cccttttcat acaggcgcac gaaggcttcg cgcaccgcgc gcgacaaccc gggatcgaga
     2581 gtgaagcgct cgcgcgacca gtcgcagctc accccggcgc
//
LOCUS       DATGEM010000049        12472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1353007, whole genome shotgun sequence.
ACCESSION   DATGEM010000049 DATGEM010000000
VERSION     DATGEM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353007"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..2889
                     /gene="ileS"
                     /locus_tag="VKQ27_01285"
     CDS             82..2889
                     /gene="ileS"
                     /locus_tag="VKQ27_01285"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HLY87592.1"
                     /translation="MNDAPTETAQRDYRDTVFLPQTSFPMRGDLPKREPQILARWEQM
                     DLWSKIRAASKGRPKFMLHDGPPYANGNIHIGTALNKIHKDVINRTRQMAGYDADYIP
                     GWDCHGLPIEWKIEEEYRARKKDKDAVPVLDFRAECRTYAEHWLAVQMAEFRRLGVEG
                     NWRERYATMDFPSEAAIAAEICKFLLNGALYRGLRPILWSSVEKTALAEAEVEYHDHT
                     SHTVWVRFPVVTASRPVLDGASLVIWTTTPWTLPGNRAIAAGADFDYAVVHVNGVADG
                     SLARVGEKLVVALELLPQVCKDVGIAAHHIDHVLKGSELPGTVCAHPWRGRGYDFDVP
                     VLLGDFVTTEAGTGLVHIAPNAGADDFELGREHGLEVADTVGDDGTYNGWVPMFAGQH
                     VYKAHDPVTAALQQAGALLARGKLVHSYPHSWRSKAPLIFRATPNWFIRLDGPERIRE
                     KALDAIAATHFVPEAGRNRLGSMVASRPDWCISRQRAWGVPITVFVDKRTGEPLRDPA
                     VVERIVAAFESEGADSWYSSPPSRFLGNDYNPNDYEQVKDIVDVWFESGSTHAFTLEA
                     RGLPWPADLYLEGSDQHRGWFQSSLLEAVGTRGVAPFKAVLTDGFVMDEQGRKMSKSL
                     GNVTAPQEVADKYGVDILRLWVMMSDTSEDLRIGPEILKQQVELYRRLRNTLRWVLGS
                     LAGFEESERVPYAEMPELEKWVLHRLAEFDARVRASVQSYDWTGVYPELHNFCATDLS
                     AFYFDVRKDAIYCDRPDSLRRRAARTVLDHLHRCLTTWLAPVLCFTAEEAWCARFGDA
                     DSVHLQQFAELPAEWRDDVLAARWETIRAIRRRITVPIEEARRANTLGSSLQAAVALP
                     LNPEHEVLLSEEQWAEIAIVSDVRIVSDPEEPAAGISAAPGEKCARCWRVLTEVGKQA
                     GHPTLCVRCTDAVDSGLVCRG"
     gene            2923..3423
                     /gene="lspA"
                     /locus_tag="VKQ27_01290"
     CDS             2923..3423
                     /gene="lspA"
                     /locus_tag="VKQ27_01290"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLY87593.1"
                     /translation="MPRNRLTLLGVVAALIVLAVDQASKWWILYKLDLPGVGQVVVLP
                     VLNFTMVWNRGVTFGLLNGFGGWSYLVLAGVAFAVVVALGMWLRRAESRVVAASLGAI
                     VGGALSNVLDRLRYGAVVDFIQAHIGAWSWYVFNVADAAIVCGVVTLLLESLLRRPDQ
                     ARSSEG"
     gene            3505..4053
                     /locus_tag="VKQ27_01295"
     CDS             3505..4053
                     /locus_tag="VKQ27_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HLY87594.1"
                     /translation="MAACAAALLGGCGSGSGQSMGQQLGHTFGFIRDAPDEFTVTTRA
                     PLAMPPAFALPPPRPGAARPQEQSQASKAEEALVPQMALGGPPASGSPGQEALVQAAG
                     PSVSNDIRAQVDAQALKDRPSESVWQKLRFWQTPAQPGIVVDPTKEAQRLRENAALGQ
                     SPESGNTPIVQPKQHGFLQGIF"
     gene            4058..5200
                     /locus_tag="VKQ27_01300"
     CDS             4058..5200
                     /locus_tag="VKQ27_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87595.1"
                     /translation="MDAPHYIQVGAVPVFTGTGVGDLTCRCGQSVLIKGYLPGNYVGI
                     RIRCFRCGEVMEMPGLPEGEILPQWAALVAPQEVPAVQTLGVAPGAVVACAAATARTY
                     ELTRPRTLPDVPLGMTRDLVEEAAAAYDRLTSGQLIAQIEASASASGDDPGAYPFAWS
                     VHRLRGLIDKPDWSWLHQNDDALAAIHVVAMHNLVQVWGHHPLFARLAARAGEAGEFL
                     RTICTFATAKLLYETGNRVGFSDDFGLHFSTALDEPLSLALLEPPLLQWRERERRNPA
                     TLKDAVIDAIGSVRSRVNRLRPGLVVLAVSIWHPDFDQMLVDAMQAALHEVGRRHRGV
                     AAVAAIMPKGLPAGALDRIGFGYAFYPIINPRFFGENPIKVGPWRN"
     gene            5266..6390
                     /locus_tag="VKQ27_01305"
     CDS             5266..6390
                     /locus_tag="VKQ27_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HLY87596.1"
                     /translation="MSGTQSLAGTTTTELKAAASAAGLRYVSDRNPGITRVRVGNSFG
                     YRHPDGSKVVDEETLTRIRKLAIPPAYEKVWICPRADGHLQAVGRDARGRKQYRYHPR
                     WREVRDEGKYGKLLVFGKVLPKVRERVEQDLSLRGLPREKVLAAIVRLLETTLMRVGN
                     EEYARTNKSYGLTTLRNRHVKVEGSSRVRLDFRGKRGTEYHIDLRSKRLAAIVRRCQE
                     LPGQELFQYLDEAGAPRTVSSEDVNEYLRAITGEEITAKDFRTWAATNLATLALRELQ
                     AFDSELPAKKHVLQAVEAVSKMLGNTPAICRKCYIHPAIFDGYLDGSLREALKRRADA
                     KLANTSSGLRAEEAAVMAFLSRSLARTGRGRGKGAMRLAA"
     gene            complement(6484..7755)
                     /locus_tag="VKQ27_01310"
     CDS             complement(6484..7755)
                     /locus_tag="VKQ27_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87597.1"
                     /translation="MAYAVPLAIVVGISTTAYADDNDSRGDEQVVMKKQTINVGGQGL
                     GAFDISFVDPRLELYILADRTNASVDLVDSEKARFLGRIGAKCPTGNPAPHFCFQGVV
                     LKPDGTANNALSGPDGDVIVDHKEIWAGDGDSRIKVIDLATQSFETTISTGGQFRVDE
                     MAYDSRDHLLAAANNADTPPFVTVFDTNAKTIKGKLVFQTGSANANVDAENGIEQPQW
                     SRDTGMFYVSVPQVGTDASVGGVSVIDPKTLAVTGTFLVKNCSPAGLAIGPNHEALLG
                     CSAAFGTPATTQSVIIDLTSTDFTHVDGAVVAKVPIGGSDEVWYDAGTNHYFLAARSN
                     TTAKGTPAPILGVVDARSHRLDQSEPSSTSSHSVAADRFSHLVFLPVGLVPANATTPD
                     PNNPCPKTGCIAVYKAHPDQNHSNEFAGMDH"
     gene            complement(8001..8798)
                     /locus_tag="VKQ27_01315"
     CDS             complement(8001..8798)
                     /locus_tag="VKQ27_01315"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HLY87598.1"
                     /translation="MKHSIRQRGFALLIVLWTLALLALLGTQVLQVSRQDTQLARNLL
                     DAAALEMTADGAVQQAIWHVLDSSTARWSADGLVHLIRIGDARVAVRIDNEADKVNPG
                     VASPELLQALLLQVGADPVSAANLAASIVQWRLGSGSAARRNTTYLRYQRAGMDYAPS
                     GAPLASIDELGSVIGMTQDLLTRLRPHLTVFTDDDPGAATRDPTVARALVLAGESEAA
                     SEMGEQFVTVTADARGADNGRIVVRVVVRINRLPQGSLFKILAYERL"
     gene            complement(8795..9382)
                     /locus_tag="VKQ27_01320"
     CDS             complement(8795..9382)
                     /locus_tag="VKQ27_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLY87599.1"
                     /translation="MMARSRERHAGGFTLLEVLVAVAVISLLIVSLVQGIHFGLLAWD
                     VEARLAGKSDGFGTVDDVLRRIVEGANAGYQDAPAPFVGRSNQLQCITALPGTGHGPS
                     LVMLEVASGHRLVLRWRPYVHAILLKSAPPVNETELLSGVSQIELSYWRSDSGWVSTW
                     QSPELPTLVRFRLVFAAGEDRHWPDIIAAPLLDPR"
     gene            complement(9379..9825)
                     /locus_tag="VKQ27_01325"
     CDS             complement(9379..9825)
                     /locus_tag="VKQ27_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLY87600.1"
                     /translation="MERGVARQNVSGPGSGFTLLEVLIALVIATMALGVLFHAGLSGL
                     RAEQTASRYEQAVSRARSRLTAAIHASPLIGGDWRGDDGDGFTWHLHVAPLASTTVRP
                     AYAATPGRLSGFPVTLYSVAVVIAWHEGSTVRQARLETQRIGREAR"
     gene            10085..11776
                     /locus_tag="VKQ27_01330"
     CDS             10085..11776
                     /locus_tag="VKQ27_01330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLY87601.1"
                     /translation="MSHRPTVYGTRHAVSAGHYLAAAAGFSILEAGGNAIDAGCAAGI
                     ALGVLQPDLVNVAGVAPIMIRLANGHVESIAGLGWWPKKLPADIFMREHGGKIPDGVL
                     RTVVPAAPDAWITALERHGTMRFADVASYAIRYAGEGFAVYPLLATSIASHEHEYRRW
                     QSNTAIFLPGGRVPKSGEKFVQSDLARTLQYMADQDRAAGPDRKAGLRAAHDAFYRGD
                     IAREIVRFQEAEGGYLAMADLDEYRSPIEPVVRRKWREHEVITCGPWCQGPALHEALA
                     LLERVGIAGLAHNSATYLHRIAECVNLAMADREYHFGDPAFVDVPIDYLLDPATIARR
                     AAAVRDDHAFGEMPAPLDLSNRSYQASAAELPKVEADTSYCCVVDRWGNAFSATPSDG
                     SGNVPVVPGLGITPSGRGSQSRPDPKHPAGVAPGKRPRLTPNPAMVVTGAGGVMPFGT
                     PGGDVQTQAMLQVLLNIFQHGMEVQDAIEAPRVASYSFPSSFAPFEYFPGRLAVEGRI
                     DPAVRADLAGRGHEVHAWPEWTWLAGSVEAVLSDPATGLIASGADPRRPAYAIAI"
     gene            12004..>12472
                     /gene="mutL"
                     /locus_tag="VKQ27_01335"
     CDS             12004..>12472
                     /gene="mutL"
                     /locus_tag="VKQ27_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLY87602.1"
                     /translation="MATIRILPPHTVNRIAAGEVIERPAAVVKELVENALDAGASRIA
                     VALDGGGIDRIEVTDDGCGMDAAGLALSVLRHATSKLADEDLVRIATLGFRGEALPSI
                     GAAARLAIVSRPREAAHASEISVEGGAVSYVAPAAGAVGTRVVVRDLFFATPGR"
BASE COUNT         2053 a         3652 c         4475 g         2292 t
ORIGIN      
        1 gcccggtggc tcgctgagcc gccggcacac tgacgcgccc cgaccaccac tgaagcggct
       61 ttggccgcca ccggacagac aatgaacgac gccccgaccg agactgccca acgggattac
      121 cgcgacacgg tgttcctgcc acagacctcc ttcccgatgc gcggcgacct gccgaagcgg
      181 gagccgcaga tcctcgcgcg gtgggagcag atggatctgt ggtcgaagat ccgcgccgca
      241 tcgaaggggc ggccgaagtt catgctgcat gacgggccgc cgtatgcgaa cggcaacatc
      301 catatcggca cagcgctgaa caagatccac aaggacgtca tcaaccgcac gcggcagatg
      361 gcggggtacg acgccgacta catcccgggc tgggactgcc acgggctgcc gatcgagtgg
      421 aagatcgagg aagagtatcg cgccaggaag aaggacaagg acgcggtgcc ggtgctggat
      481 ttccgcgctg agtgccgcac gtatgccgag cactggctgg cggtgcagat ggcggagttc
      541 cggcggcttg gcgtggaggg caactggcgc gaacgctatg ccacgatgga ctttccgtcc
      601 gaggccgcca ttgccgcgga gatctgcaag ttcctgctga acggcgcgct gtatcgcggg
      661 ctgcggccga tcctgtggtc ctcggtggag aagaccgcgc tggccgaggc cgaggtcgag
      721 taccacgacc atacctcgca cacggtgtgg gtgcggttcc cggtggtgac ggcgagccgg
      781 ccggtgctgg atggggcgtc gctggtgatc tggaccacca cgccgtggac gctgccgggg
      841 aaccgggcga tcgcggcggg ggcggacttc gattacgccg tggtgcatgt gaacggcgtg
      901 gcggatggct cgctcgcgcg cgtcggcgag aagctggtgg tggcgctgga gctgctgccg
      961 caggtctgca aggacgtggg gatcgccgcg catcacatcg accatgtgct gaaggggagc
     1021 gaactgccgg gcacggtgtg cgcgcatccc tggcgcgggc ggggctacga cttcgacgtg
     1081 ccggtgctgc tgggggactt cgtcacgacg gaggcgggca ccgggctggt gcatatcgcg
     1141 ccgaatgcgg gcgccgatga tttcgagctt gggcgcgaac atgggctgga ggtggcggac
     1201 accgtggggg atgacggcac ctacaacggg tgggtgccga tgttcgccgg tcagcatgtc
     1261 tacaaggcgc atgatccggt gaccgcggca ttgcagcagg cgggggcgct gctggcgcgg
     1321 ggcaagctgg tgcactcgta tccgcattcc tggcggtcga aggcgccgct gatcttccgg
     1381 gcgacgccga actggttcat ccggctggat gggccggagc gcatccggga gaaggcgctg
     1441 gacgcgatcg ctgccacaca tttcgtgccg gaggcggggc gcaaccggct gggctcgatg
     1501 gtggcgagcc ggccggactg gtgcatcagc cgccagcgcg cctggggcgt gccgatcacg
     1561 gtgttcgtcg acaagcgcac cggcgagccg ctgcgcgatc cggcggtggt ggagcggatt
     1621 gtcgcggcgt tcgagtccga gggggcggac agctggtact cgtcgccacc gtcccggttc
     1681 ctcgggaacg actacaatcc gaacgattac gagcaggtga aggacatcgt ggacgtgtgg
     1741 ttcgagtcag gctcgacgca tgcgttcacg ctggaggcgc ggggactgcc ctggccggcg
     1801 gacctgtatc tggaagggtc ggatcagcat cgcgggtggt tccagtcgtc gctgctggag
     1861 gcggtgggca cgcgcggggt ggcgccattc aaggcggtgc tgaccgatgg gttcgtgatg
     1921 gatgagcagg gacgcaagat gtcgaagtcc ctgggcaacg tcacggcacc tcaggaggtc
     1981 gccgacaagt acggcgtcga tatcctgcgg ctttgggtga tgatgtcgga tacgtcggag
     2041 gatctgcgga tcgggccgga gatcctgaag cagcaggtgg agctgtaccg gcggctgcgg
     2101 aacacgctgc ggtgggtgct ggggagcctg gccgggttcg aggagagcga gcgggtcccg
     2161 tatgccgaga tgccggaact ggagaagtgg gtgctgcacc ggctggcgga attcgatgcg
     2221 cgggtgcggg catcggtgca gagctatgac tggaccgggg tctatcccga gctgcataat
     2281 ttctgcgcga ccgatctctc ggcgttctac ttcgatgtgc ggaaggacgc gatctactgc
     2341 gaccggccgg attcgctgcg gcggcgggcg gcgcggacgg tgctggatca tttgcaccgc
     2401 tgcctgacga cgtggctcgc gccggtgctg tgcttcacgg cggaagaggc ctggtgcgcg
     2461 cggttcggcg atgcggacag cgtgcatctg cagcagtttg ccgagctgcc ggcggagtgg
     2521 cgggatgatg ttctggcggc ccggtgggag acgatccggg cgatccggcg gcggatcacc
     2581 gtgccgatcg aggaggcccg gcgggcgaat acgctggggt cctcgctgca ggccgctgtg
     2641 gctttgccgc tgaacccgga gcacgaggtg ctgctgagcg aggaacagtg ggcggagatc
     2701 gccatcgtgt ccgatgtgcg gatcgtgtcg gacccggaag agccggcggc aggcatcagc
     2761 gcagcgcccg gcgagaagtg cgcgcggtgc tggcgggtgc tgacggaggt ggggaagcag
     2821 gccgggcatc cgacgctgtg cgtgcgctgc accgacgcgg tggattcggg gttggtttgc
     2881 cggggttgac gggcgggagt tgctccgcca tcgcacgcca tgatgccccg taatcgcctt
     2941 accctgctcg gcgtcgtggc ggcgctgatc gtgctggccg tggatcaggc gtcgaagtgg
     3001 tggatcctgt acaagctgga tctgccgggt gtcggccagg tcgtcgtgtt gccggtgctg
     3061 aacttcacca tggtctggaa tcgcggcgtc accttcgggc tgctgaacgg attcgggggg
     3121 tggagttacc tggtgctggc cggggtcgcg ttcgccgtgg tggtggcgct cgggatgtgg
     3181 ctgcgccggg ccgagtcgcg ggttgtcgcg gcctcactgg gggccatcgt cggcggcgcg
     3241 ctgagcaatg tgctcgaccg gctacgctat ggcgcggtgg tggacttcat ccaggcgcat
     3301 atcggggcgt ggtcgtggta cgtgttcaac gtggcggacg cggccattgt gtgcggcgtg
     3361 gtgaccctgc tgctggagag cctgttgcgc cggccggacc aggcgcgttc atcggagggg
     3421 tgagattctt gcggctgacg ctccactccg gctaataggc taacgatgcc gttatctttg
     3481 atccgccgga cgacaccgtt gctagtggct gcctgcgctg ctgccctgct gggcggctgc
     3541 ggttcgggct caggccagag catgggccaa cagcttggcc atacctttgg tttcattcgc
     3601 gacgcgcccg acgaattcac agtcacgacg cgcgccccgc tcgccatgcc gcccgctttc
     3661 gcgctgccgc cgccgcggcc gggggcggcg cgcccgcagg agcagtcaca ggcttcgaag
     3721 gcggaagagg cgctggttcc gcagatggcg ctgggtgggc cgccggcgtc cggcagtcca
     3781 ggccaggagg cactggtgca ggcagcgggg ccttcggtat cgaacgacat ccgcgcccag
     3841 gtggacgcgc aggcgttgaa ggaccgtcca tcggagagcg tctggcaaaa gctgcgcttc
     3901 tggcagacgc cggcgcagcc tggaatcgtg gtggatccga cgaaggaagc gcagcggctg
     3961 cgcgagaacg cggcgctggg gcagagcccg gagagcggca acacgccaat cgtgcagccc
     4021 aaacagcacg gattcctgca ggggatcttc tagcggcttg gacgctccgc attacatcca
     4081 ggtgggggcg gtgccggtgt tcacgggcac cggtgtcggc gatctgacct gccgttgcgg
     4141 gcagtcggtg ctgatcaagg gctacttgcc ggggaattat gtcggcatcc ggatcaggtg
     4201 cttccgatgt ggcgaggtca tggagatgcc cggcctgccg gaaggcgaga tcctgccgca
     4261 gtgggccgcg ctggtcgcgc cgcaggaggt gccggcggtc cagacgctcg gagtggctcc
     4321 cggtgccgtt gttgcgtgcg ccgcggccac ggcgcgcacg tatgagctga cgcggcctcg
     4381 gaccttgccg gatgtgccgc ttgggatgac gcgggatctg gttgaagagg ccgccgctgc
     4441 ctatgaccgg ctgaccagcg gccagctgat tgcgcagatc gaggcgtccg ccagtgcttc
     4501 aggcgacgat cccggggcgt atccatttgc ctggtcggtt catcgattgc gggggctgat
     4561 cgacaaacct gactggagct ggctgcacca gaacgatgac gcgctcgcgg cgatccatgt
     4621 cgtggcgatg cacaatctgg tgcaggtttg ggggcatcat ccgctgttcg cgcggttggc
     4681 ggcgcgggcg ggtgaggcgg gggagttcct gcgcacgatc tgcacgttcg cgacggcgaa
     4741 gctgctgtat gagacgggaa atcgcgtcgg gttttccgat gatttcgggc tgcatttctc
     4801 caccgcgttg gatgagccat tgtcgctcgc gctgttggag ccgccgctgc tgcaatggcg
     4861 ggaacgcgag cgacgcaatc ccgcgacgtt gaaggatgcg gtgatcgacg cgatcggatc
     4921 ggtgcggagc agagtcaatc gcctgcggcc tgggctggtg gtgctcgcgg tgtcgatttg
     4981 gcatcccgat ttcgaccaga tgctggtgga tgcgatgcag gcggcattgc atgaagtggg
     5041 acggcggcac cgcggtgtcg cggcggtggc ggccatcatg ccaaaggggc tgccggcggg
     5101 cgcgctggat cggatcggct tcggttatgc gttctatccg atcatcaatc cgcgattctt
     5161 tggcgagaat ccgatcaagg tcgggccgtg gcgcaattag gcggctaatt ctgtcgcgcg
     5221 gttgtcacta catttttggg gacggcgatc cacatcgtgt gtcccatgtc cggcacacag
     5281 tcgctcgcag gtaccaccac cacggaactg aaggctgcgg caagtgccgc cggattgcgc
     5341 tacgtcagcg atcgcaatcc tggcatcacg cgcgttcgcg tgggcaacag ctttggctat
     5401 cggcatccgg acggcagcaa ggtcgtcgat gaggagacgc tgacgcggat ccgcaagctc
     5461 gcgattccgc cggcatatga aaaggtgtgg atctgtccga gggccgatgg acatctgcag
     5521 gctgtgggtc gtgatgcgcg cgggcgcaag cagtatcgct accatcctcg ctggcgcgaa
     5581 gttcgcgacg agggcaagta cggcaagctg ctggtgttcg gcaaggtcct gcccaaggtc
     5641 agggagcggg tggagcagga cctgtcactc cgtggcctgc cgcgggagaa ggtgctcgct
     5701 gcgatcgtgc ggctgctgga aacgacgttg atgcgcgtcg ggaacgaaga atatgcgcga
     5761 accaacaaga gctacggcct gaccacgctg cgcaaccggc acgtgaaggt tgaggggtca
     5821 agccgggtcc ggctcgactt ccggggcaag cgaggcaccg agtatcacat cgatctgcgg
     5881 agcaaacgtc tggccgctat cgtgcggcgg tgccaggaac tgccgggcca ggagctgttc
     5941 cagtatctcg atgaggcggg cgcgccgcgc accgtgagtt cggaggatgt caacgaatac
     6001 ctgcgggcca tcaccggcga ggagatcacc gcgaaggact tccgcacctg ggcggcgacc
     6061 aatcttgcca ctctggcgct gcgcgaattg caggccttcg actcggagtt acctgcgaag
     6121 aagcatgtgc tgcaggcggt ggaggcggtg tccaagatgc tcggcaacac gccggcgatc
     6181 tgccgcaagt gctacatcca tccagcgatc ttcgatggct acctcgatgg ttcgctgcgg
     6241 gaggcgctga agcggcgggc ggatgccaag ctcgcgaata catcgtccgg gctgcgggcg
     6301 gaggaggccg cggtgatggc gtttctgagc cggagtttgg cgcgcacagg ccgcggtcgc
     6361 ggcaaaggtg cgatgcggct ggcagcttag cgtcgctgtt gttttagcgg acgtgtgaat
     6421 ggtgttttgc gctgaccgac gggttccact gccgtgcagc agtggaaccc gtcaggtcag
     6481 acctcagtga tccatgccag cgaactcatt actgtgattt tggtcaggat gcgctttata
     6541 gacggcaatg catccagtct ttgggcacgg attattggga tcgggcgtcg tcgcattcgc
     6601 gggcactaaa cctacgggca ggaataccag gtgcgagaac ctgtcagctg caacggaatg
     6661 cgacgaggtg gaagacggtt ccgactggtc aagccgatgg cttcgcgcat cgaccacgcc
     6721 aaggattggc gcgggtgtgc ccttcgcagt cgtgttgctg cgtgcagcca ggaagtagtg
     6781 gttggtaccg gcatcgtacc atacctcgtc agacccgccg atgggcacct tggcaaccac
     6841 cgcgccatcc acgtgggtga agtcggtact ggtgaggtca ataatgaccg actgcgtcgt
     6901 cgccggtgtg ccaaacgccg cgctacagcc aagcagtgcc tcgtggttcg gcccgatcgc
     6961 aaggccggca ggtgagcagt tcttcaccag gaacgtaccc gttaccgcca acgtcttcgg
     7021 atctatgacc gacacgccgc ccacggaagc atcggtccca acctgtggca ccgacacata
     7081 gaacatcccg gtgtcgcgcg accattgcgg ctgttctatc ccgttttccg catcgacgtt
     7141 ggcgtttgcc gaccccgtct ggaacacaag cttgcctttg atcgtcttgg cattggtgtc
     7201 aaataccgtg acgaagggtg gtgtatcagc gttattggcc gctgccagca aatggtctcg
     7261 cgaatcgtat gccatctcat cgacccggaa ctggccaccg gtcgatatcg tggtttcgaa
     7321 cgactgcgtg gcgaggtcga tgaccttgat cctgctgtca ccgtctccag cccagatctc
     7381 cttatggtcc acgatgacgt cgccatcagg acccgaaagt gcattgtttg ctgtgccgtc
     7441 cggcttcagc acgacacctt ggaagcagaa gtggggggcc ggattacccg tggggcactt
     7501 ggccccgatg cgtccgagga agcgggcctt ctccgagtcg accaaatcga ctgaggcatt
     7561 ggttctgtcg gccaggatat agagctcgag ccgtggatca acgaagctga tatcgaatgc
     7621 cccaagcccc tgaccgccca cattgatcgt ctgcttcttc atgacgacct gctcatcgcc
     7681 acggctgtca ttatcgtccg cgtaagcggt agtcgagatg ccgacgacga tagccaacgg
     7741 caccgcatac gccatcaggc gtttacgacg actcatttcc tgccctttcc ctagtgttga
     7801 ttggtcgacg cggttcggag cggcagtctt ttatccgttt ccaccccgag atcagtgaat
     7861 aggcgatttt ggccttccgc aaatcacttt atggacattt catcatgcat tcaggcatgc
     7921 cgcgacaacc ttccggccga gaaatcagat atcattttgc aaggtaaatc tccgctgatg
     7981 ataccgttgc ttgctgtgtt tcagagccgt tcgtatgcga ggattttgaa caggctgcct
     8041 tggggaagcc gattgatccg gacgacgacg cggacgacga tgcggccgtt gtccgccccg
     8101 cgggcgtcgg ccgtcacagt cacgaactgc tcgcccatct cgcttgctgc ctcggactcg
     8161 ccggccaaga cgagagctcg ggccactgtc ggatcgcgtg tcgcggctcc cgggtcatcg
     8221 tcggtgaaaa ccgtgaggtg cggcctcaag cgcgtcagca ggtcctgcgt cataccaatg
     8281 acagacccga gttcgtctat actggcgagt ggcgcgccgg acggcgcata atccatgccc
     8341 gctcgctgat aacgcaggta agttgtgttc cgccgcgctg cggaaccgct gcccaggcgc
     8401 cattgcacaa tcgacgcggc caaattcgcg gcggaaaccg gatccgcgcc aacctgcaac
     8461 aggagcgcct gcaacagttc gggcgaagcg acgccagggt tcaccttgtc ggcttcattg
     8521 tcgatacgca ccgcgacgcg tgcatcgcct atcctgatca gatggacgag gccatcggcg
     8581 ctccagcgcg ctgtcgagct gtcgagcaca tgccagatcg cctgttgcac ggcgccatcc
     8641 gctgtcatct ccaaggcggc cgcgtcgagc agattacggg cgagctgcgt gtcctggcgg
     8701 cttacttgca gcacttgcgt gccgagcagt gcaagcaacg caagcgtcca taggacaatc
     8761 agcaatgcga agccgcgctg acgaatgctg tgcttcatcg gggatccagc aatggcgctg
     8821 ctatgatatc gggccagtgg cgatcctcgc ccgcggcaaa cacaagccga aagcgcacca
     8881 gggtgggcaa ttcgggcgac tgccatgtgc tcacccagcc actgtcggac cgccaatacg
     8941 acaattcgat ttgagaaacc ccgctcaaca actcggtttc attgaccggg ggggcggact
     9001 tgagaaggat cgcgtgcaca taagggcgcc agcgcaacac cagccgatgg ccgctggcga
     9061 cctccaacat gaccaggctt ggtccatgtc cggtgccagg cagggctgtg atgcattgaa
     9121 gctgatttga acgtccgaca aacggtgccg gggcatcttg gtaacccgcg tttgcgcctt
     9181 ccactatgcg gcgcagcaca tcgtcgaccg tgccaaaccc atcgctcttg ccggcgagcc
     9241 gggcctccac gtcccacgcg agcaggccaa aatgaatgcc ctgtaccagg ctgacgatca
     9301 gcagcgagat caccgctacc gcgaccagga cttccagcaa ggtaaacccg ccggcgtgtc
     9361 tttcgcggga gcgagccatc atcgggcctc ccggccgatc cgctgagttt cgaggcgagc
     9421 ttgccttaca gtgctgcctt cgtgccacgc gatcaccacc gcgaccgagt aaagggtcac
     9481 aggaaagccg gacaaacggc ctggcgtcgc ggcatacgcc gggcggacgg tggtgctcgc
     9541 cagtggcgcc acgtgcaggt gccaggtgaa gccgtcgcca tcgtcgccgc gccagtcacc
     9601 gccgatgagg gggctggcgt gtattgcggc cgtcagtcgc gaccgggcgc gggacacagc
     9661 ctgttcatac cttgatgctg tctgctcggc tcgcaagccg ctcagcccgg catggaaaag
     9721 gacgccaagc gccatggttg ctatgacgag ggcgatcagc acctcgagga gggtgaagcc
     9781 ggaccccgga ccgctgacat tctggcgcgc gactcctcgc tccatgaaag gcctcttgat
     9841 cgcatcgtgc ggattggtcg acgcacgcag cgtgccccag ggacgttcct tttgcacgcg
     9901 ctgttctggc gcaacctgcc gcgcctcggg ggcagcccga ccaccggcat cgggatgctc
     9961 tcagcctatg cgcaagctgg cgacggctct ctcagccacc acattgggtc atgggcgacg
    10021 ctggacctgt gttccgcttt gccgcacgct tcgctgctga gccattcgcc gcaggagctt
    10081 cgccatgtct catcgcccta ccgtctatgg cacgcgtcat gccgtttccg ccggccatta
    10141 tctggcggcg gcggcggggt tctcgatcct ggaggcggga gggaatgcga tcgatgcggg
    10201 gtgcgcggcg ggcatcgcgc tgggggtgct gcagccggac ctcgtgaacg tggctggtgt
    10261 ggcgccgatc atgatccggc tggcgaacgg gcatgtggag agcatcgccg gcctgggatg
    10321 gtggccgaag aagctgccgg ccgatatctt tatgcgagag catggcggaa agatcccaga
    10381 cggggtgctg cggacggtgg tgccggcggc gccggacgcg tggatcaccg cactcgagcg
    10441 gcatggcacg atgcggttcg ccgatgttgc ttcctatgcg atccggtatg ccggggaggg
    10501 gttcgcggtc tatccgctgc tggcgacgtc gatcgcatcg catgagcatg agtaccggcg
    10561 gtggcagtcg aataccgcga tcttcctgcc gggcgggcgg gtcccgaagt ccggcgagaa
    10621 gttcgtgcag tcggatctgg cgcggacgct gcaatacatg gcggatcagg atcgcgccgc
    10681 gggaccggac cggaaggccg ggctgcgggc ggcgcatgat gcgttctacc ggggagatat
    10741 cgcgcgggag attgttcggt ttcaggaggc cgagggcggt tacctcgcta tggcggacct
    10801 ggacgaatac cggtcgccga tcgagccggt ggtgcggcgg aagtggcgcg agcatgaggt
    10861 gatcacctgc gggccctggt gccaggggcc ggcgctgcat gaggctctgg cgttgctgga
    10921 gcgggtgggg atcgcggggc tggcgcataa ctcggccacg tacctgcatc ggatcgccga
    10981 gtgcgtgaac ctggctatgg cggatcggga ataccatttc ggcgatccgg cgtttgtgga
    11041 cgtgccgata gattatctgc tcgatccggc gaccattgcg cgccgggcgg cggcggtgcg
    11101 cgacgatcac gcgtttggcg agatgccggc gccgctcgat ctgtcgaaca ggtcgtatca
    11161 ggcgagtgcc gcggaactgc cgaaggtgga ggcggatacg tcctattgtt gcgtggtgga
    11221 ccggtggggg aatgcgttct cggccacgcc ttcggatggg tcggggaatg tgcctgtcgt
    11281 gcccgggctg gggatcacgc cgtccgggcg agggtcgcag agccggcctg atccgaagca
    11341 tcctgcgggg gttgcgccgg gcaagcggcc tcggctgacg ccgaatccgg cgatggtggt
    11401 gaccggggcc gggggcgtga tgccgttcgg cacgccgggc ggggatgtgc agacacaggc
    11461 gatgctgcag gtgctgctga acatcttcca gcatgggatg gaggtgcagg acgcgatcga
    11521 ggcgcctcgg gttgcgtctt attcgttccc gtcctcgttt gcgccgttcg agtatttccc
    11581 ggggaggctg gcggtggagg ggcggatcga tcctgccgtg cgggctgatc tggccggacg
    11641 ggggcacgag gtgcacgcct ggccggaatg gacgtggctg gctgggtcgg tggaggcagt
    11701 gctttcggac ccggcgacgg ggttgatcgc ttctggggct gatccgcggc ggccggctta
    11761 cgcgatcgct atctgagcct ttttggcaac gccggcgcgg ttggtcctgc tggattctta
    11821 acacaaagat cgcaaaggac gcgtagaaca caaagaaggc gtttatttag gcgcttcgcg
    11881 ccggctgctg ccaaggtctg cgcgaggcgc tgaaaatacc tttgtgttct ctgcggtccc
    11941 ttgtggcctt tgtgttgaag ccggtctttg ttcacgctgc gtccagcgga ttagtattgg
    12001 gcgatggcca cgattcgcat cctgccgccg cataccgtca atcgcatcgc cgccggcgag
    12061 gtgatcgagc ggccggctgc ggtggtgaag gagctggtcg agaatgcgct cgacgcgggg
    12121 gcgtcgcgga tcgctgtagc gctcgatggc ggcggtatcg accggatcga agtcaccgac
    12181 gacggatgcg gcatggatgc cgcggggctg gcgctttcgg tgctgcggca tgccacgtcc
    12241 aagctggccg acgaggatct ggtgcggatc gcgacactcg ggttccgggg ggaggcgctg
    12301 ccctcgatcg gggcggcggc gcggctggcc atcgtgtcac ggccgcgcga ggcggcgcat
    12361 gcttcggaga tctcggtcga gggcggggcg gtgtcgtatg tggcgccagc ggcaggcgct
    12421 gttggcaccc gggtggtggt gcgcgacctg ttcttcgcca cgccggggcg gc
//
LOCUS       DATGEM010000050        30810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1352593, whole genome shotgun sequence.
ACCESSION   DATGEM010000050 DATGEM010000000
VERSION     DATGEM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 30810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30810
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352593"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1613)
                     /locus_tag="VKQ27_01340"
     CDS             complement(144..1613)
                     /locus_tag="VKQ27_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLY87603.1"
                     /translation="MRSESAGRSAIWAVLGQGVGQALSLLIFLVIARFVAQASFGIVA
                     VSLASIEFIRRALLDPITFVVTAKPKVEERDFSVCFSILVVLSVAATSLLLVCAPLVA
                     WLIGSPQAGSTLRSVAVMLLGFGVAGSQGAWLVRGMQFRALALRSIASVVAGGIVGIT
                     MALNGFDLWSLVAQQVTINLVGAAILWLTSGWRPHFTLSWADARDSFRRARHISMSAI
                     WTSIANDADLFFASAFFGPTAAGVYNAGKRILLTANLLLVSTISAVTLPILANLEHDR
                     ERGASFLDGLRMASVGTAPAFIGLAVASQDLVHIILGPHWMAVGPIIAAMAFSGYAFS
                     IGQFATSVLLVAQRAHLDSRTSAISALLNVVAFVLVVRFGPVALAATVSITTLCVVPI
                     RVRFALKELDLHWPQVFEALLPSIIASSAMAISVLSLRLLLPADLAAILRLTASISYG
                     IIVYALWLRIFAPNLFRMTMAASSEAIGIRAPSRATEAD"
     gene            complement(1996..2223)
                     /locus_tag="VKQ27_01345"
     CDS             complement(1996..2223)
                     /locus_tag="VKQ27_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87604.1"
                     /translation="MTAPPFVVVVRRSDGTTQTVFRDADLRRAASEAFLVTDKLCRTP
                     ERPSRSPARAVTIECDGRLELSITVTPGRPL"
     gene            complement(2220..2807)
                     /gene="radC"
                     /locus_tag="VKQ27_01350"
     CDS             complement(2220..2807)
                     /gene="radC"
                     /locus_tag="VKQ27_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLY87605.1"
                     /translation="MVDILQPLEPVLASRIAETLIDRFGSLAAVLAADPTARARAVPD
                     HPVAMAHLQTMHDAVLHALRTEMESAPLLTSMKATVDYLRLLIAQELAEQFRVLFLDT
                     HGRLIREEMISRGSVHEAPVYPREIMRRALELGATILILVHNHPSGDPAPSQQDLRIT
                     QRLINAGHELDIAIHDHIIVTRCGWASLRALGAIQ"
     gene            complement(2759..3235)
                     /locus_tag="VKQ27_01355"
     CDS             complement(2759..3235)
                     /locus_tag="VKQ27_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87606.1"
                     /translation="MTHLEVFYALLGLCTGYALLCGGAPERIVALLFLGATALMPFVL
                     SGQHGRYSSVEAGALTVDAVMYVALLWVALRAERYWPIWMSAMQGVQLLGHGIGLLGF
                     KLLNLSYAIIDHTICYPMMLLLAISVRRHQIRRARSGVDASWSTSSSRWNPFSLHA"
     gene            complement(3279..3623)
                     /locus_tag="VKQ27_01360"
     CDS             complement(3279..3623)
                     /locus_tag="VKQ27_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87607.1"
                     /translation="MRNEKMAMIRQVRDDLLPAEVSFDETLGGMGKLLQTICITRLDG
                     GLPMYVGQEAIGHLTAAAAMLGEARGRIVSAHQCFAKDRSILVPEVAWGDGTDCPSQA
                     SAEPLVPLRAVG"
     gene            complement(3683..4234)
                     /locus_tag="VKQ27_01365"
     CDS             complement(3683..4234)
                     /locus_tag="VKQ27_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY87608.1"
                     /translation="MGNDRFDKLTERQRECLRLVHHGHEVKEIARELGVGPSAVVERL
                     RAARKTLGVESSRLAARMLAHEEGNATYIRHVDMPSWLADEASAAALLAPSRAEGEGE
                     AHRDRVMEAAAMFEPLPIFLPARSFPWPLRTREQASNDLTSAERLVLSVSLTVVFTIA
                     ASLSVIAVILLMQFLIELAKHGG"
     gene            complement(4318..5295)
                     /locus_tag="VKQ27_01370"
     CDS             complement(4318..5295)
                     /locus_tag="VKQ27_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY87609.1"
                     /translation="MTTPISPLRMGAREWALLLMLSALWGGSFFFFKVLVAEVPPFTV
                     VLGRVGIAAVALNAYLLLRRDHMPWDAGIWRGFLMLGLLNNVIPFSLIVFGETRISSG
                     LASILNAMTPVFTVLAAHALTSKEKLNGTKAAGVLCGLGGVAVLVGPAAFGQSGGADL
                     VGEACCLLAALSYAFAGIFGRRFRGIPPIKVATGQITASTLILIPLSLLVDRPWTLAA
                     PSPHAWAAFFSIALLCTALAYLIYFRILATAGATNISLVTFLLPVSALILGALVLHER
                     ITAGALIGMGLIGVGLAAIDGRLPAAIWRKVRKAIVGRILSPSSRVGGV"
     gene            complement(5367..6023)
                     /locus_tag="VKQ27_01375"
     CDS             complement(5367..6023)
                     /locus_tag="VKQ27_01375"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLY87610.1"
                     /translation="MVDRQSRLHDMVTRQIVARGVRDAAVIAAMRAVSREAFVDPAQA
                     AFAYDDTPLSIGAGQTISQPYIVAVMLEAARIATTDVVLEVGAGSGYLAALMGRLAAR
                     VIAIERQPRLATAARQRLADMGYTNVEILTGDGTEGCPGAAPFDAIIVSAGGPAIPLP
                     LREQLAVGGRLVMPIGRGADQNLIRLTRSNVDVFDEEEFGAVRFVPLIGSHGWPASDL
                     "
     gene            6632..7378
                     /locus_tag="VKQ27_01380"
     CDS             6632..7378
                     /locus_tag="VKQ27_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY87611.1"
                     /translation="MQAQLNVAIVSQNEIEREGLRRILTEQAFSVIGVHRDSGELDLD
                     IFDGAADAIPLVIVDSTKEDDGLDACRFIHARWPDCKIVVMAADCQSQMVAEAFHAGI
                     DGYVGKHISCASLTEMLKLVALGEKMIPSQVVFDLASLKSEKGMSQLKVSTGEANLSD
                     REVEILRGLIRGEPNKVISRRLTVTEATVKVHVKSILRKLGVMNRTQAAIWGMSRGLT
                     DMSPGPVRAEGNVEKRPSAETFEASRAYMA"
     gene            complement(7392..9443)
                     /locus_tag="VKQ27_01385"
     CDS             complement(7392..9443)
                     /locus_tag="VKQ27_01385"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLY87612.1"
                     /translation="MDGNGHGPRAIALVGPAGAGKTSLAEALLFAAGAIDRLGSVGSG
                     SSIGDASPEARARGSSTELNLMRLEWMGEPFVLIDSPGSVGFAADAATAIATADLALV
                     VVDPDPNRAPLVEPTLRWLESLGLPHALFVNKIDQARGSVAELLAALQPMSTAALVAR
                     QIPINDGNGIAGFVDLALERAYHYRPGRPSEPIPLASDLQAEEMAARNHMLELLADHD
                     DTLLEQLISDEMPSLETIFRDLARETQAGWVVPVLFGSASNGFGVRRLLKMLRHDAPS
                     AATTAARLGLDQPAVRVVKISHGNAIGRLALARVFGAPLGEGADLAVPAGPSARAGAL
                     FTLQGGTTTKVNRVEAGDVVGIAKTDQLLRGDIAGIGCTPSGTIAEIELPPDNCALAI
                     AAKEHKDEVRLSTALNRLAEEDPGLHWGQDPGSRETLLHGVNDEHLAVALDRLKRRYG
                     VVVSAHRPHVAYKESVRKPTSQRGRHKKQSGGHGQFGDVVIELRPLERGQGFQFEDKI
                     SGGAIPRQWIPAVEQGVRDALAKGPLGFPVVDVAVALVDGSFHSVDSSELAFRTAGRI
                     AMTEALAAAVPYLLEPVSHVVIHAPGSAASRVNSAIATRRGQMLGMVPKDGWSRWDTI
                     EVLLPEAELQGFEAELRSLSQGMARYEARFDHLAEVSSRLAGEIVLRARDPCARETAG
                     A"
     gene            complement(9582..11048)
                     /locus_tag="VKQ27_01390"
     CDS             complement(9582..11048)
                     /locus_tag="VKQ27_01390"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HLY87613.1"
                     /translation="MVTALPLAATLAAAALSSAAAPDLVLTGAIDGKNHQTYQEAPFS
                     VPADVTRVTIRFDYDKSNHTVIDLGLLDPQRFRGWSGGSKALLTLSPSDATPSYLPGP
                     VVAGRWRLLMGIPNARPGSHSAYTASIWFDRGTGHAASDAATPPPLRSGPGWYRGDFH
                     MHSAHSDGSCLSQSGVRVPCPAFRTVESAATAGLDFIALSDHNTVSHLNDLRELQPYF
                     DKLLLIPATEITTFHGHIQAIGLDRFIDFRLGTPVVPNPTALADDAARAGAILSINHP
                     NSPSGEMCMGCGWSAPDTDYDRIQAIEAVNGRIADGSLSGIPFWYARLNEGHHLTGIG
                     GSDSHDPDAPDGKPPVGHPTTVVHAANLSQAAILDGIRAGDVFVDTQGTRDRLLEVEA
                     HAGNAHVPMGGTIDLHGPLTLDVRVAHADGARIELVHRGETLAALSALVTGPDVHQSL
                     VLSPEQSCGWIAINVRDAQGAALLVGNPVYVRCAAETH"
     gene            complement(11051..13792)
                     /locus_tag="VKQ27_01395"
     CDS             complement(11051..13792)
                     /locus_tag="VKQ27_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY87614.1"
                     /translation="MRYQGLVAVSAIALACAAQPASAQTRSFDVPAQAAVTAIPAFAK
                     QAGVQIVAPAAHLKGRTTQAVKGTMDVDAGLRQLLSGTGLIVGARTGQTISLRAEEPT
                     TSDATPPSSLAEAGSAGDNSIVVTGRAGAGGRTKAQTSYAITTLTADALRTRAPSSVT
                     EALKSVPGFWVEASGGEGSGNIRARGIPVDGFGSINLEEDGLPVQHDPALGYLNADQA
                     FRIDESIDRVEVVRGGPSSIFTSNAPGGVVNFITRKPTDEFSGLIKYEYGPTSNLNRF
                     DGWVGGPLGAGWKFDIGGFYRNETGVRDPGFTGNRGGQIRADLTHDLGHRGHVAFSFK
                     RVDDRAIFYTGIPLTKDANGDIVAVNGFDGHYGTTASPETAHLTLRNGNGGVVNFDNT
                     DGTFVKLNQYSAMADYEIFDGWTLTNKARYRTSDTVRNGVYPSALQKASDYVASMQGG
                     LLAAYPGATSVQLRYTDSGQPFDIANQNGNGLILQNSARPVTVNEKEFLDDLRLTHEF
                     EIGGMRHNIALGGYFAHINESFGRYSAATLQDVSDNSRLLDLVALNAAGQVVGSLTQN
                     GVTRYGSEFANGSGKQDTFAFYASDEWQVTRQLRIDGGLRYEHVNTTGSSEGSKTVNL
                     GDPSTLADNSALVGNGVFTPFDRDFHHLGWTIGADWQFTPKAGVFARYTSTSRLPSVS
                     DFITNPTNSQVVVQKIKMWEGGVKYASDPFSVYATAFDTDYGSYAIGNYIFNPVTGII
                     TQTLEHANTRTYGVELETVVRPVHWFELALNGTVQQPKFRDLRYTDSAGVFHDFAGNQ
                     LLRVPKLSLRATPAVTLLNDRLRAQVDIEHYGSRYADAANTQKLPSYNVINASVRFAV
                     SPKVTLYAYGDNLNNSLGLTEGNPRAGELSSGQAGNQFFIGRPILGRSFRFAAMYQF"
     gene            complement(13885..14901)
                     /locus_tag="VKQ27_01400"
     CDS             complement(13885..14901)
                     /locus_tag="VKQ27_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLY87615.1"
                     /translation="MKDDRLPTTGREPAQNIDETAAVWTARLDRGLTSEERAALDQWL
                     AGDSRRIGALARARAVWTHADRVRSLGPAATQDPPEPSFWERIDRRTAMLGGGAIAAS
                     LVAGVTIPRLLDRGRRLKTATGEIRRVPLGEGSVVTLDTDSVVETAFSKERRDVRLLS
                     GVAFFEVAHDSARPFVVEARDLLVRAVGTAFSVRNLPGQPVSVVVSEGRVALSRTIGQ
                     GSATPLELGANAQAIVLPGSRLDAGGVAHALTPDDLTRSLSWRDGMLAFEGETLAQAA
                     QRFRRYGGPAIVIDDAALARQPISGLFAASDPRGFAQAIAVSFDAHVAEDSGKVHLKS
                     ERRR"
     gene            complement(14849..15454)
                     /locus_tag="VKQ27_01405"
     CDS             complement(14849..15454)
                     /locus_tag="VKQ27_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY87616.1"
                     /translation="MAHAPDDRSLWLATNVLPHERSLRDWLARFAFLDEDRVDDLVQE
                     TYAVLATRAEVASIRAPRAYAFQVAKSILLQDLRRSRVVAIGSVADLEALDHAADLPS
                     PEQHAIGRDELNRVARAIAAMPKQTRRAFQLRRIEGLSQREVAQVLGLSENTVEKHIV
                     RGIKLLMQQFGRGGKASSDASMPRDEGRPFADDRARTRAEY"
     gene            complement(15513..16028)
                     /locus_tag="VKQ27_01410"
     CDS             complement(15513..16028)
                     /locus_tag="VKQ27_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLY87617.1"
                     /translation="MPELTPIPQHEFVDAVHDLAGQLTQADWRPSFLIGIGRGGLVPA
                     VFLSHAIGLPTLSVDYSSQVPDFAAEPLLKLARRTQQGEHLLFVDDINDTGRTIVHLR
                     AVLAEAGAVQSNIRFATLIDNIRSLARVEYAARVIDRAVTKDWFVFPWEAVAPDATIL
                     EDAAAVPERTA"
     gene            complement(16021..17061)
                     /locus_tag="VKQ27_01415"
     CDS             complement(16021..17061)
                     /locus_tag="VKQ27_01415"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HLY87618.1"
                     /translation="MTDLDAFITGLPKAELHLHIEGSLEPELMFALAERNKLSIPFTS
                     VEEVRAAYQFSRLQDFLDIYYAGADVLRTERDFQDLAEAYFDRAAADGVVHAEIFFDP
                     QTHTARGIPFGVVAEGLLAGMAAAQARHGLSSQLILCFLRHLDEADALATLAEAEPWL
                     DRIAGVGLDSSELGHPPSKFARVFARAADMSLKRVAHAGEEGPAAYVWEALDLLGVDR
                     LDHGNRSLDDPRLVARLAREAMTLTVCPLSNVKLCVVDDIAGHPIDRMLDAGLRATIN
                     SDDPAYFGGYIADNYRAVAKGRGIGRDRLAMLARNSLLGSFLDEAALTQHLAGLDAYL
                     AASAPAKERAHA"
     gene            complement(17312..17521)
                     /locus_tag="VKQ27_01420"
     CDS             complement(17312..17521)
                     /locus_tag="VKQ27_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87619.1"
                     /translation="MRGASHQVFGAKRDADEVIDRGHGLLRVVEADLSEREWLAAELR
                     DYWIVAQPAHGPCPNGTSGGTSGPA"
     gene            17789..19192
                     /locus_tag="VKQ27_01425"
     CDS             17789..19192
                     /locus_tag="VKQ27_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLY87620.1"
                     /translation="MTYRLLIDGALVDGAVTVEVVNPATGRPFAIAPRADVVQLERAV
                     AAAKRAFPTWSRTRWAERQALLAAIADVLADKGEEFARLLTQEQGKTLTEARGEMEGT
                     IAAFRYFATLELSPRVLRDTATDRIVEYRHPLGVVAAIVPWNYPLLMLALKMAPALLA
                     GNTLIAKPAPTTPLATLRLGEIMAEMVPAGVFQILIDENDLGPLLAAHPDIAHVSLTG
                     STATGRMVMGAASGTLKRLVLELGGNDAALLLDDADIASVAPKIFESAMANAGQVCFA
                     IKRVYAPRALVGPLCDALTELARAAVIGDGMIEGTTMGPVHNRAQFDKLAALLDKTRG
                     EGRIVAGGEVLPGEGYFIAPTVVRDLGDDALVVREEQFGPILPVLGYDNLDDAIARIN
                     ASEYGLGGSVWTRDVERGIAVAAWIESGTVWVNKPIDLPFDIPVGGVKQSGMGRQQGM
                     AGLEEFTQPRIVNAALG"
     gene            complement(19200..20495)
                     /locus_tag="VKQ27_01430"
     CDS             complement(19200..20495)
                     /locus_tag="VKQ27_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLY87621.1"
                     /translation="MVGVVLLGILGWMVGQPGPTAFAKGNPVDLAQYRGSSPVGVPAE
                     LANADPLTRGKYLTDAADCQGCHTTKGGTPFAGGRPFTLPFGTLYTPNITPDPDTGIG
                     KWSDTDFLKAVHKGIAPDGTRLYPAFPYTSYTMLTDQDVLAIKAYLFSLKPVRQEAKP
                     NTFGFPYNQRWLMAIWSTLFNSDKRFQPIPEQSPQWNRGAYLVEAASHCGECHTPRNL
                     LQAMNTRQKFAGGQAEGWNAYNITPDRVSGTGAWSAQDLAAYLAKGHALGRGTASGPM
                     REAVDLSLSKLTPGDIGAMVTYLQSVPPIHSSQLPQLAGPAPVAPKAGPTDNPVGKRL
                     FEGNCASCHAWNGNGALIQEAQLTGNRAVNDVTAINVAQMILNGAGTPNGGRPYMPSF
                     AVAYTDREIASVANYVTARFGSKPSTITPKQVAELRAQN"
     gene            complement(20531..22747)
                     /locus_tag="VKQ27_01435"
     CDS             complement(20531..22747)
                     /locus_tag="VKQ27_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY87622.1"
                     /translation="MLHDRPDPSPASTGGSRGLSRRTLLGAGAAVGGGLLLGIELIGG
                     RSAEAKDPRPQGGATFNAFVRIARDGAITMIMPAVEMGQGTYTMMATLIAEELDVDLA
                     QVTVEHAPPDQKDYGNPIFVAQITGGSTSTMGWYLPLRKAGATARAMLVGAAAAGWNV
                     DPASLRTESGVVHHDASGRSAPYGRLADRAAAIPAPKEPILKDPKSFRLIGKPLKRID
                     TPDKVNGKVQYGIDVMLPGMKFGTLAACPVFGGKVRHVDDSQARAVPGVRQVVVLDDL
                     VAVVGDHMWAAKQGLAALAIDWDYGPNAHAMQEDLYAAQAKAANGSGVTAQKVGDAPA
                     HLKDGTVYEDEFELPYLAHAPMEPINGTAHFKGDSCEIWVGTQAPVMAQEGAARALGL
                     KPEQVTINNHLIGGGFGRRLEVDGVVKVARIAQHVEGPVKIVWSREEDIQQELFRPIY
                     HDRLRARVENGRITAWHHRVTGPSIMARWLPPAFQKGIDPDAVDGAIEQPYDFDHILI
                     EYIRHETPQVPVAFWRGVGPNSNIFSAERFINHIAHQTGVDPVEFRRGMLKKSPRALA
                     VLNLAAEKAGWGQPLAAPSGGGRAGRGIALLAGFGSFLACVAEVVVPDDGDVQIKRIV
                     CAADVGKMINPNTLEAQVQGGTIFGMSAILHGEITIDHGRVLQSNFHDYRVLRIDEMP
                     RIETYFVPSEEKPGGIGEPGTVVVQPAIANAVFDATGVQLKRMPINRALLAKGAKA"
     gene            complement(22728..23183)
                     /locus_tag="VKQ27_01440"
     CDS             complement(22728..23183)
                     /locus_tag="VKQ27_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY87623.1"
                     /translation="MTILKINGQERTVDVPADMPLLWVLRDVIGLTGTKFGCGIAQCG
                     ACTVHLDGSPVRSCQLPVGVIGDRAITTIEAIGDTPAGAKIQKAWLDLEVVQCGYCQS
                     GQIMSAAALLASNPSPDDADIDTAMAGNICRCGTYVRIREGIKHAARSA"
     gene            23410..24549
                     /locus_tag="VKQ27_01445"
     CDS             23410..24549
                     /locus_tag="VKQ27_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLY87624.1"
                     /translation="MTGFAFQYGPRLLAGPGVSTRIGELVSARHVLVVTDGQVRALAL
                     ADAMLDVLRCEGRSVTIFDAVEADPSRSTLEAAVEVGWGAEAVIGFGGGSPMDVAKLT
                     AYLLGSGDDLDAIWGVGNAAGTGLPLVLVPTTAGTGSEATPVAIISLPGGEKRGVSSS
                     ALIAHHAVLDPTLTLGLPRHVTAATGIDAMVHAIEAYTSAHLKNPVSDMLAKEALGLL
                     SSHLLRACEVPDDIDARGAMLLGAHLAGVAFSNAPVAGVHALAYPLGGRFHVPHGLSN
                     ALMLPHVLGHNMQAAMPLYAELAPIVDPSLALLGGQARAQGLVEALRGLSMAAGMPQR
                     LREVGIGVEHLEMLAADAMQQERLLRNNPCPIELADAQRLYEAAL"
     gene            24546..24977
                     /locus_tag="VKQ27_01450"
     CDS             24546..24977
                     /locus_tag="VKQ27_01450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLY87625.1"
                     /translation="MNREPLRPRSAYRHWTRISTRWHDNDVYGHVNNTVYYGWFDTAV
                     NAWLVEAGLLDVERGDPIGLVAETGCRYAAPVAFPQPVEVGLTLARLGSSSVTYHLGV
                     FAEGTDHAAAEGHFTHVYVERESRRPAPLPEEWRHLIETLA"
     gene            complement(25074..25886)
                     /locus_tag="VKQ27_01455"
     CDS             complement(25074..25886)
                     /locus_tag="VKQ27_01455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87626.1"
                     /translation="MMVKPNHGTALITGASTGIGAIYADRLARRGHDLILVARNQEKL
                     EALARKLREETGRIVEVAAADLGRKDDLARIETLLKTDERIGVLVNNAGVGATAPLLS
                     ADVDEMEAMITLNVTALTRLTYAAAPGFVSRGGGTIINIASIVAIGPEVLNGVYGGSK
                     AFVLALTQSLHHELADKGVRVQAVLPGATATDFWDIAGTPIHYLPGEIVMMATDMVDA
                     SLVGLDMGELVTIPSLPDAADWNAYEAARQILKPNLSHAQPADRYMARSVAA"
     gene            26037..26978
                     /locus_tag="VKQ27_01460"
     CDS             26037..26978
                     /locus_tag="VKQ27_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLY87627.1"
                     /translation="MMSLAAASVFEFANVQSGEALYDIRILSETGGPVRSSTGIIVDS
                     TPLGAARLDTMLVCGGVGIPDASPALLAFIRGSTSAVRRLASICTGAFVLAQAGLLDG
                     RKATTHWLLARELQAGFPGVRVEDDRIFIRDGSIWTSAGMTAGVDLALALLEADQGEE
                     LARAVARKLVLYHRRAGGQSQFSTLLELEPKSDRIQHALAYARSHLKAPLSVEELADV
                     ANLSPRQFSRAFRSETGQSPAKAVEHLRVEAARVMMEQGRHPVDVVARETGFADRERM
                     RRAFLRAFGQPPQAVRRNARGGMAPSPDAVADSVHSM"
     gene            27094..28221
                     /locus_tag="VKQ27_01465"
     CDS             27094..28221
                     /locus_tag="VKQ27_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87628.1"
                     /translation="MTYAIDRLVFVPDDVDLQRSPLAGQIPAETYVLGAFNPGLARLA
                     NGNLLLMVRVAEALRTPNFDGHVHAIRWDGEQGRYTLDAWPLDLADTADPRKFFLRGG
                     GWPIIALTSLSWLLPVEFSPDGLERVAIHYDRAIAPQYSFQCYGIEDARISRFDLQYL
                     MTTCSVSPERQATTLYSSTDAVDWRFEDIVLDHQNKDMLIFEGRIGGQYWAQTRPLGD
                     VYFAYPPGSVWRSGPSINLATSPDAHHWKPWREPGIRPRTDSLATARIGGGAPPILTR
                     DGWLSLWHGVEPQEIIGIYRTYWSIMDRDDPSRIVRSGSAPLIEPAPELTRPIEHQMY
                     LSDVVFTTGIADGGEHYIVASGEADLACRITHVPKSAFGLD"
     gene            complement(28281..30089)
                     /locus_tag="VKQ27_01470"
     CDS             complement(28281..30089)
                     /locus_tag="VKQ27_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87629.1"
                     /translation="MRLTAADRPAATVAPESRRGTFRGLLGLFAAAPVPRWTTLTLVV
                     LGLISSLAETLGITLVVVFFYSAMGQIDQAVPVQGLLGGPLREATRWFASSTEIAAVI
                     LLLIIARGGLSFLHGLLSARISEQISEDVRNRVHRQYLRVAYPFIQRHEQAHLMEVLG
                     TETWLISGAHSSLTRIVVNICSILVFTAALISLSWRITLVAVLGSLLISIGLRYLSRS
                     ARQLGIRVKYVHQLLGEHMLMTLQGMRTIRAYGQEALHQERFEHASADARRTAIALSR
                     LMAQLHPLTELGYIGVLCVIIALADRGQTGFPITLAAIALLYRLQPHLRELESNLLGL
                     AQIEPRLASVRAMLATDDKDYPSPGHRPIRTLASNIRFENVGFLYDNAGDAALTDISF
                     EIPAGKTTALVGASGAGKTTIVNLLLRLYQPTAGTILIDGVPLEDIRRTDWLDLLAIA
                     GQDVDLIEGTVIDNIRMANGAASDDDVRSALRVAGIADTINALPDGYSTWIGQQGLRF
                     SGGQRQRLGLARAILRDPDFLILDEAMSALDKRLEDGIRHGIDRRFADRTRLLITHRT
                     ETLYDVDHVVCLDQGRVVAEGPPDEVFARPSVRSLP"
     gene            complement(30080..>30810)
                     /locus_tag="VKQ27_01475"
     CDS             complement(30080..>30810)
                     /locus_tag="VKQ27_01475"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87630.1"
                     /translation="DADDLWAPTKIERQLDALLAGGDRVGLVYTWYQLIDGEGRVLHH
                     GGCPLHEGDVLDDIFSGNFIGNGSAALVRRQTLIDARGFESGLRTADAQGCEDLLFYC
                     RAAERHGFAVIPDYLVGYRYLPDNMSSNLPRMLRSWMLVKDEMEERHPDRHAALDVGL
                     RNYGAWLLFKALTTGRLAYALSLIDLLVRQEPRIAVSVLFFRLPLTLLNAVRWRLQAR
                     LRRLLCPPERRSPALFINEPGQCA"
BASE COUNT         5426 a         9598 c        10376 g         5410 t
ORIGIN      
        1 aacttgggat ataatcgtcg ccatgttcgc ggagatccta ccgccccgaa ctatgacgac
       61 tttactgcga tgagcggggt tcgattctct ctctgagagg ccggcgcgcg cgaccttgtt
      121 tggctgtgtt gacctgccag gggtcagtca gcttcggtcg cgcgtgatgg agcacgtatc
      181 ccgatcgctt cgctgcttgc agccatcgtc atccggaaga gatttggtgc gaaaatgcgc
      241 agccacagcg catagacgat gatcccatag ctaatgctcg cggtgaggcg cagtattgct
      301 gcaaggtcgg ccgggagcaa aaggcgaagg ctgagtacac tgatggccat ggccgaggat
      361 gcaatgatcg atggcagcag cgcctcgaaa acttgaggcc agtgcaggtc gagttccttg
      421 agagcaaagc gcacccggat gggcaccacg cacaaggtcg tgatcgacac ggtcgcggcg
      481 agcgcgactg gcccgaagcg aacgactagg acaaaagcca ccacgttgag aagggccgag
      541 atcgcggagg tccgtgagtc cagatgcgcg cgctgggcaa ccagcaggac ggacgttgca
      601 aattgaccga tcgagaaagc gtagccgctg aaggccatgg ccgcgataat agggccgacc
      661 gccatccaat gcggccccag aataatgtgc acaaggtctt gcgaggccac cgccagccct
      721 atgaaggctg gcgctgttcc cacgctcgcc atgcgcaagc cgtcgagaaa cgaagcgccg
      781 cgctcgcggt catgctccag attggcgagg atcggcagcg tcacggcgga aatcgtgctc
      841 acgagcagca gattggcggt caggagaatg cgcttgcccg cattgtagac gcctgcggcg
      901 gtggggccga aaaaggcgga ggcgaagaac aggtcggcat cgttggcgat cgatgtccag
      961 atcgcgctca tggagatgtg ccgcgcacga cgaaaactgt cgcgagcatc cgcccaggac
     1021 agcgtgaaat ggggccgcca tcccgacgtc agccagagga tcgcggcgcc gaccagattg
     1081 atcgtgacct gctgggcgac aaggctccat aggtcgaagc cgttgagcgc catggtgatt
     1141 ccaacgatcc cacccgccac gaccgacgcg atcgagcgaa gcgccagcgc gcggaattgc
     1201 atcccccgta ccagccatgc tccctgcgag ccggcgacac cgaaccccag gagcatgact
     1261 gcgaccgacc tcagtgttga gccggcctgt ggacttccaa tgagccaggc aacgagtggt
     1321 gcacagacca ggagcaaact ggtcgccgcc accgacagga caacgagaat gctgaagcaa
     1381 actgagaaat cgcgctcctc aaccttaggc ttggcggtga cgacaaaggt gatgggatcg
     1441 agcagcgcgc gtcgaatgaa ttcgatcgac gcaaggctta cggcgacgat gccgaaactg
     1501 gcctgcgcca caaagcgcgc gatgacgaga aagataagga gggacagcgc ctggcctacc
     1561 ccctggccaa gcactgccca aatcgcgctc cgccccgcgc tctcgcttct catgtgcact
     1621 gcgatattac ctcaccaaat tgcagctgat tgtttgctgc cgcggtggga gagggcagac
     1681 atggagtggg ccataagggc tcaaaccatt ttaaagcccg caatcgtgtc gatggatcac
     1741 gcatgagtgg ccgtgatgtg cggccgtggc cgaaccagca tgtacatctc ccaatgcccc
     1801 ttgttctcgc gtgtgggccc cgtgcggcga ggacaagctg acatccttaa ggaaatgcct
     1861 gtccgatctg actggttatt tgggatgaca gccacccgcg caggccgcgg tgcctatggg
     1921 ggggcgcgct agtgcggttt acagccatat tgatgggccg aaagcgccgg tatctgcgtt
     1981 ggagcgcagt ccaatttaga gcggcctgcc cggagtgacc gtgatgctga gctccagccg
     2041 tccgtcgcat tcgatcgtca cggcgcgcgc cgggcttcgc gaggggcgct cgggtgtccg
     2101 gcagagcttg tccgtgacca ggaaggcttc gctggctgcc cggcggagat cggcatcgcg
     2161 gaacacggtc tgcgtggttc cgtcgctacg gcggacgacg acgacgaagg gcggtgcagt
     2221 cattgaatgg ctcccaatgc tctcaggctg gcccagccgc agcgggtgac aatgatgtgg
     2281 tcgtggatcg cgatatcgag ctcatggccg gcattgatca ggcgttgtgt gatacgcaga
     2341 tcctgctggc ttggcgccgg gtcaccgctg ggatggttgt ggaccaggat caggatggtg
     2401 gcccccagct cgagcgcgcg tcgcatgatc tctcgcgggt agaccggggc ttcatgcacc
     2461 gacccgcgtg agatcatttc ctcgcggatg agccgtccgt gcgtgtccag gaagaggaca
     2521 cggaattgct cggcaagctc ctgggcgatc aggagacgca gatagtcgac ggtcgccttc
     2581 atgctggtga gcaggggagc gctctccatc tcggtccgca aggcatgcag caccgcgtca
     2641 tgcatcgtct gaagatgcgc catcgcgacg ggatgatcgg gaaccgcgcg cgcccgcgcg
     2701 gtcggatcgg cggcaaggac cgccgcgagc gacccgaacc gatcgatcag cgtctcggct
     2761 atgcgtgaag cgagaacggg ttccagcggc tggaggatgt cgaccaggaa gcgtcgacac
     2821 cgctcctcgc ccgccgaatc tggtgccggc gcacgctgat cgccaacagc aacatcatcg
     2881 gatagcagat cgtatgatcg atgatcgcat atgacaggtt gagcagcttg aagccgagca
     2941 ggccgatgcc gtgcccgagc aactgcactc cctgcatcgc gctcatccat atcggccaat
     3001 acctttcggc acgcaaagca acccaaagca gcgcaacata catgactgcg tcgaccgtca
     3061 gcgcaccggc ctccacagag gagtagcgac catgctggcc tgacaagacg aacggcatca
     3121 gggccgtggc gccgagaaac agcagcgcca caatccgttc cggcgcaccg ccacacagca
     3181 gcgcgtagcc ggtacagagg cccagaaggg cgtagaaaac ctccagatgc gtcatgccct
     3241 cgcagaggga tcacgatgcc cttccggggt caagatggtc atcccaccgc gcgcagcggc
     3301 acgagcggtt cggcgctggc ttgcgatggg caatcggtgc cgtcacccca cgccacttcc
     3361 ggcacgagga tcgagcgatc cttggcgaag cactggtgcg ccgaaacgat ccggccgcgg
     3421 gcttcgccca gcatcgccgc cgcggcggtg agatggccga tcgcctcctg cccgacatac
     3481 atcggcaggc caccgtcgag gcgggtgatg cagatcgtct gcaggagctt gcccattccg
     3541 cccagcgtct catcgaacga gacttcggcc ggaagcagat catcgcgaac ctggcggatc
     3601 atcgccatct tttcgttacg catgattccc tcctctccgc gaggcactcc ccgcggttct
     3661 agatgcagcc atggggttga tctcagccgc cgtgcttcgc gagttcgatc aggaactgca
     3721 tcagcaggat gacggcaatt accgacaatg acgccgcgat agtgaagacc accgtcaggc
     3781 tgacggacag caccaacctc tcggccgacg tcaggtcatt gcttgcttgc tccctcgttc
     3841 ggaggggcca ggggaagctt cttgccggga ggaagatggg gagcggctcg aacatggccg
     3901 ctgcttccat cacccgatcc cgatgggctt ctccctcgcc ctccgctctc gatggagcaa
     3961 gcaatgccgc cgccgaggcc tcgtcggcaa gccagctcgg catgtccaca tgccgtatgt
     4021 aggtggcgtt tccttcctcg tgagcgagca tgcgggcggc cagccgactg ctttccacgc
     4081 ccagcgtctt gcgggcggct cgcaggcgtt cgacgacggc gcttggcccg acgccgagct
     4141 cgcgcgcgat ctccttcacc tcgtggccgt ggtgcacgag gcgaaggcat tcccgttgcc
     4201 gctcggtcag cttgtcgaag cggtcgtttc ccactcgcgc atcgccctta tcccacccga
     4261 gcgtgaacct gtgcgaaagc ggtagggaaa tgcaaccgga atggcgacca aagtgtctca
     4321 gaccccgccg acgcgactcg agggggacag aattcggccg actatggcct tgcgcacctt
     4381 acgccagatc gccgcgggca ggcgaccatc gatcgccgcc aggccgacac cgatcaggcc
     4441 catgccgatc aacgcgccgg cggtgatgcg ctcatggagc acgagggcgc ccaatatcag
     4501 cgcgctcacc gggagcagga aggtgacgag cgaaatattg gtcgcccccg ccgtcgccag
     4561 gatccggaag taaatcagat aggcgagcgc ggtgcacaac agggcaatgc tgaagaaggc
     4621 cgcccatgcg tgggggctgg gcgcggcgag cgtccacggt cggtcgacca gaagagagag
     4681 gggaatgagg atcaacgtgc tggccgtgat ctggcccgtc gcgaccttga tcggcgggat
     4741 gccgcggaaa cgccggccga agatgccggc aaaggcatag ctcaaagcgg cgagcaggca
     4801 gcaggcctcg ccgaccagat cggccccgcc ggattggccg aatgcggccg ggcccaccag
     4861 gacggcaacg ccgcccaatc cgcagagtac accggcggct ttggtgccgt tcagcttttc
     4921 cttgctggtc agcgcatggg ccgcgagcac ggtgaagacc ggagtcatgg cattgagaat
     4981 cgaggcgagg ccgctggaga tgcgggtctc gccgaacacg atcagcgaga aagggatcac
     5041 gttgttgagc aggcccagca tcaggaatcc ccgccagatc ccggcatccc aaggcatatg
     5101 atcgcgccgg agaagcagat aggcattgag agcgacggcg gcgatgccga cccggccgag
     5161 tacgacggta aagggcggga cttcggccac cagcaccttg aagaagaaga atgagccacc
     5221 ccacaaggcg gagagcataa gcagcagagc ccattcccgg gcccccatcc tcaacggtga
     5281 aatgggcgtc gtcatgcagg cctcgctccc ttgtcggcgc gcaccatagg cggcgcgcgg
     5341 gaccggcagc tacccgtatc ttgcggtcaa agatctgacg ctggccagcc atggctgccg
     5401 atcaacggca cgaaacggac cgcgccgaat tcctcctcgt cgaagacgtc aacgttcgat
     5461 cgggtgaggc ggatcaggtt ctgatccgcg ccgcgtccga tcggcatgac gagcctgccg
     5521 ccgacggcga gttgttcgcg cagcgggagc ggaatggcgg ggccgcccgc cgacacgatg
     5581 attgcatcga acggagcggc acccgggcag ccttcggtgc cgtctcctgt caggatctcg
     5641 acattggtat agcccatgtc ggcgagtcgc tggcgggctg cggtcgcgag ccgtggctgg
     5701 cgttcgatgg cgatcacccg cgccgccagt cgccccatca gcgcagcgag atatcccgat
     5761 cccgctccga cttcgagcac cacgtctgtc gttgcgatgc gtgctgcttc gagcataacc
     5821 gccacgatgt agggctgcga gatggtctgc cccgctccga tcgacagggg ggtgtcgtca
     5881 taggcaaagg cggcctgcgc tggatcaacg aaggcttctc gcgacacggc ccgcatcgcg
     5941 gcgatcacgg cggcatcacg gacaccacgg gcgacgatct gccgggtcac catgtcatgc
     6001 agacgggatt gtcgatcgac catgttcacc ctcggcgtct ccgcttgcgc cgaaaatagg
     6061 aggcgcaaat tggaactgcc agccgcagcc tgcgatacca cgttggctgt tcctcgcggg
     6121 gcaattttgt cgtccggatc gacctgctga tggtccgcgt gtcgggctag gtccgggcac
     6181 gaacgccatg cttcggacgt tcagaccggg caggtttgcg gcgatccctg taagctggtg
     6241 gacagttgcg tgatcatcaa ttctcggcca ggagaatatt aacatagtta acgtggatga
     6301 ctatccaaag gaatagcaac gttggcgccg cgatgcttct cctataatcc ttttgtatgt
     6361 cgtggcgata tcccgatgaa cgcctacccc gcttgcctgc aagctgtacg atatcgcgtt
     6421 gctaagttat cgaattcggg tcgctcgtcg aatatctgga cgatcggctt gttgttagct
     6481 gtactgctca acaggatcat accgtagggg acgatgcatc tggatggata ggtcacgtgc
     6541 ctgcttcgct ggcgcgggcc gattggtcgg atgcctctcg cgagcactat gctctgcgcc
     6601 gccgcctatg gtgaaaccgg ggggacatgt tttgcaggcg caactgaatg tcgcgatcgt
     6661 cagccagaac gagatcgagc gtgaagggct ccgtcgcatc ctgacggagc aggccttctc
     6721 ggtcattggt gtccatcggg attctggcga gcttgatctg gatatcttcg acggcgcagc
     6781 cgacgccatt ccgctcgtga tcgtcgattc gaccaaggag gatgatgggc ttgatgcctg
     6841 ccggttcatc cacgcccgct ggccggactg caagatcgtc gtgatggcgg ccgactgcca
     6901 aagccagatg gtcgccgagg cgtttcacgc cggaatcgac ggttatgtcg gcaagcacat
     6961 atcctgcgcc agcctgaccg agatgctcaa gctcgtcgct ctgggcgaga agatgattcc
     7021 ctcccaagtg gtgttcgatc tggcttcact gaagtccgag aagggcatga gccagctcaa
     7081 ggtttccacc ggcgaagcca atctctcgga tcgagaggtt gagattctgc gcgggctcat
     7141 ccggggagaa ccgaacaagg taatttcgcg gcggctgacc gtcaccgagg caaccgtgaa
     7201 ggttcacgtc aaatcgatcc tgcgcaaact gggcgtgatg aacaggacgc aggccgcgat
     7261 ctggggaatg agccgggggc tgacggacat gtcgcccggt cccgtcaggg ccgaagggaa
     7321 tgtcgagaag cggcctagtg ccgaaacgtt cgaagcgagc cgcgcctaca tggcgtgact
     7381 gttggagcat gtcaggcccc cgcggtctcg cgggcgcagg gatctcgtgc gcgcaggacg
     7441 atctcgccgg cgagccgcga gctgacctcg gcgagatggt cgaaacgcgc ttcgtaacgg
     7501 gccatgccct ggctcagcga ccgcaattcg gcctcgaacc cctgaagctc ggcttcggga
     7561 agcagcacct cgatcgtgtc ccagcgcgac cagccgtcct tcggcaccat gccgagcatc
     7621 tgaccgcgtc gtgtggcgat cgcggagttg acccgtgagg cggcgctgcc cggcgcatgg
     7681 atcacgacgt gcgaaacggg ttcgagcaga tagggaacgg cggccgccag cgcctcggtc
     7741 atcgcgatac gccccgccgt gcggaaggcc agttcggaac tgtcgacgct gtggaaggag
     7801 ccatcgacca gcgccaccgc cacatccacc accgggaagc cgagcggccc cttggccagt
     7861 gcatcgcgca cgccctgctc gacggccggg atccattgcc ttgggatcgc gccgccgctg
     7921 atcttgtctt cgaactgaaa accctgccca cgctccagcg gccggagttc gatcacgaca
     7981 tcgccgaact ggccatgacc gccagattgc ttcttgtggc gcccacgctg gctcgtcggt
     8041 ttccggaccg attccttgta ggcgacatgg ggccggtgcg ctgagacgac cacgccatag
     8101 cgccgtttga ggcggtcgag ggctacggcc aaatgctcgt cattgacgcc gtgcaggagg
     8161 gtttcgcgcg atccggggtc ttgtccccaa tgcaggccgg gatcctcctc ggcgagccgg
     8221 ttgagcgcgg tcgagagacg aacctcgtcc ttgtgttcct tcgctgctat cgcgagcgcg
     8281 caattgtcgg gcggcagctc gatctccgcg atggtgcctg aaggcgtgca gccaatcccc
     8341 gcgatgtcgc cgcgcagcag ttggtcggtc ttggcgatgc cgacaacgtc gccggcttcg
     8401 acgcggttca ccttggtcgt ggtgccgccc tgcagcgtga acagtgcgcc ggcccgcgcc
     8461 gaagggcccg ccgggacggc cagatccgcg ccttcgccga gaggtgcgcc gaacacgcgc
     8521 gcgagggcga gccgtccgat cgcgttacca tgcgagatct tgacgacgcg aaccgccggc
     8581 tgatccagtc cgagccgggc cgccgttgtc gcggcggacg gagcatcatg ccgcagcatc
     8641 ttgagcagcc tgcgcacgcc gaacccgtta gacgcggatc cgaacagcac ggggacaacc
     8701 catccggcct gcgtctcgcg cgccagatct ctgaagatcg tctccaggct cggcatctca
     8761 tcggaaatga gctgctcgag cagggtgtcg tcatggtcgg cgagcagctc cagcatatgg
     8821 ttgcgggcgg ccatctcctc ggcctgcagg tccgatgcga gcgggatcgg ctcggacggt
     8881 cggccgggcc ggtaatgata ggcacgttcc agcgcgagat cgacgaagcc cgcgatcccg
     8941 ttgccatcgt tgatggggat ctggcgtgcc acgagtgcgg cggtgctcat cggctgaagc
     9001 gcggcgagca gctcggccac gcttccccgc gcctggtcga tcttgttgac gaacagcgca
     9061 tgagggaggc ccaggctttc gagccagcgc agcgtgggtt cgacaagggg ggcgcggttc
     9121 ggatcgggat cgacgaccac gagcgcgaga tcggcggtgg cgatcgcggt cgccgcatcg
     9181 gcggcgaaac ccactgatcc ggggctgtcg atcaggacga acggctcgcc catccattcg
     9241 aggcgcatca ggttgagttc ggtcgagctc ccgcgtgcac gcgcttcagg gcttgcgtcg
     9301 ccgatgctgc tcccgctgcc gaccgagccc agccggtcga tcgcgccggc tgcgaagagc
     9361 agcgcttcgg caaggctggt cttgcccgca cctgcggggc cgaccagcgc gatggcccta
     9421 gggccatggc catttccatc cacagcctca ctcctctcct gccaggacgg ttcgcccgtt
     9481 cgtgactaga gagtcggacg ttggcggccg gaacgcaagg gtgcggatgg aagaccgttt
     9541 ggggagtgag cccgtcctgg ggcaatgtcc cagtcggaga gttagtgagt ctccgctgcg
     9601 caacggacat agaccggatt gccgaccagc agcgccgctc cctgtgcgtc gcgaacgttg
     9661 atcgcgatcc atccacagga ctgttcgggc gacagcacga ggctctgatg cacgtccggg
     9721 cctgtcacga gcgcggacag cgccgccagc gtctcgccac gatgaacaag ctcgatccgc
     9781 gcgccatcgg catgggcaac gcggacgtcg agcgtcaacg gtccgtgaag atcgatcgtc
     9841 ccgcccattg gcacgtgggc gttgccggca tgtgcttcga cctcgagcag ccgatcgcgc
     9901 gtcccctgcg tatcgacgaa cacgtcgccc gcgcggatgc cgtcgagaat ggcggcctgc
     9961 gagaggttgg cggcatggac gacggtcgtc gggtggccga cgggtggctt gccgtcagga
    10021 gcatcggggt catggctgtc gctgccgccg atgccggtga ggtgatgccc ctcgttgagg
    10081 cgcgcatacc agaaagggat tccggagagg gaaccgtcgg cgatgcggcc gttgacggct
    10141 tctatcgcct ggatgcggtc atagtccgtg tccggagcgc tccatccgca tcccatgcac
    10201 atctcgcccg aaggcgagtt agggtggttg atcgacagga tcgcgccggc gcgcgccgcg
    10261 tcgtcagcca gcgcggtggg gttgggcacc accggcgtcc cgaggcggaa atcgatgaag
    10321 cggtcgagcc cgatcgcctg gatatgaccg tggaaggtcg tgatctcggt cgccgggatc
    10381 agcagaagct tgtcgaaata gggctggagc tcgcgcaggt cgttcaggtg cgacacggtg
    10441 ttgtggtcac tgagcgcgat gaaatcgagg cctgccgtgg cggccgattc caccgtgcgg
    10501 aacgccgggc agggaacgcg cacgcctgac tggctgaggc agcttccgtc actgtgcgcg
    10561 ctgtgcatgt ggaagtcgcc gcgataccag cccgggccgg aacgcagcgg cggcggcgtg
    10621 gcagcgtcgc ttgcggcgtg gcctgtaccg cgatcgaacc agatgctggc ggtgtatgcg
    10681 ctgtggctgc cggggcgggc gttgggaatg cccatcagca atcgccagcg gccggccacg
    10741 acgggccctg ggagatagga gggggtggca tcgctcgggc tgagcgtcag cagcgccttg
    10801 gagccgccgc tccatccccg gaaacgctgg ggatcgagca ggccgaggtc gattacggtg
    10861 tggttcgact tgtcatagtc gaagcggatc gtgacgcggg ttacatccgc aggaacgctg
    10921 aacggcgcct cctgataggt ctgatggttc ttgccatcga tggcgccggt caacaccagg
    10981 tctggcgccg ccgcgctcga aagcgcggca gcggccaggg tggcggccag ggggagcgcc
    11041 gtcaccattc ctagaactga tacatcgcgg cgaagcggaa cgagcgtccg aggatcgggc
    11101 gtccgatgaa gaactgattg cccgcctgtc cgctcgaaag ctcgccggcg cgcggattgc
    11161 cttcggtcaa ccccagcgaa ttgttgaggt tgtcgccata ggcatagagc gtgaccttgg
    11221 gcgagaccgc gaagcgcacg ctcgcgttga tcacgttgta cgagggcagc ttctgcgtgt
    11281 tggcggcgtc ggcgtagcgg ctgccataat gctcgatgtc cacctgcgcg cgcaggcgat
    11341 cgttgagcag ggtgacagcc ggggtggcac ggagcgacag cttgggcacg cggagaagct
    11401 ggttgcctgc aaagtcgtga aacacgcccg ccgagtccgt gtagcgcaga tcgcggaact
    11461 tgggctgctg gacggtgccg ttcagagcca gctcgaacca gtggaccggg cgaaccacgg
    11521 tctccagctc gacaccgtag gtgcgcgtgt tggcatgctc gagggtctgg gtgatgatgc
    11581 cggtgaccgg gttgaagatg taattaccga tggcatagct gccataatcc gtgtcgaacg
    11641 cggtcgcgta aacgctgaac ggatcggagg catatttgac gccgccttcc cacatcttga
    11701 tcttctgcac caccacctgc gagttggtcg ggttggtgat gaagtcgctg acgctcggca
    11761 ggcgtgacgt cgaggtgtag cgtgcgaaca cgccggcctt gggtgtgaac tgccagtcgg
    11821 cgccgatcgt ccagccgaga tggtggaagt cgcggtcgaa gggcgtgaac acgccgttgc
    11881 caaccagcgc actgttgtcc gccagcgtcg agggatcgcc gagattgacg gtcttgctgc
    11941 cctccgaact gccggtcgtg ttgacgtgct cgtaacgcag cccgccatcg atgcggagct
    12001 ggcgggtcac ctgccactcg tccgaggcgt agaaggcgaa ggtgtcctgc ttgcccgatc
    12061 cgttggcgaa ttcggacccg tagcgggtga cgccgttctg cgtcaggctg ccgacgacct
    12121 ggccggcggc gttgagcgca acgagatcga gcagccgcga attgtccgag acatcctgca
    12181 gggtcgcggc ggagtagcgg ccgaagctct cgttgatatg cgcgaaatag ccgccgagag
    12241 cgatattgtg gcgcatgcca ccgatctcga actcgtgggt gaggcgcaga tcgtcgagaa
    12301 actccttctc gttcaccgtc acggggcgcg ccgaattctg gaggatcagg ccattgccgt
    12361 tctgattggc gatgtcgaag ggttggccgg agtcggtgta gcgcagctgc accgaggtcg
    12421 cgcccggata ggcggcaagc aggccgcctt gcatcgaagc gacatagtcc gatgcctttt
    12481 gcagcgccga gggatagacg ccgttgcgca ccgtgtccga cgtccggtag cgcgccttgt
    12541 tggtgagcgt ccagccgtcg aaaatctcat agtcggccat cgccgaatat tggttgagct
    12601 tcacgaaggt gccgtcggtg ttgtcgaaat tgacgacgcc accattgccg ttgcgcagcg
    12661 tcaggtgcgc cgtctcgggg ctggcggtgg tgccgtagtg gccgtcgaag ccgttgaccg
    12721 cgacgatatc accattggca tccttggtga gcggaatgcc tgtgtagaag atcgcgcgat
    12781 catccacccg cttgaagctg aaggcgacat ggccgcgatg cccgagatca tgggtgaggt
    12841 cggcgcggat ctggccgccg cgattgccgg tgaagcccgg atcgcgcacg cccgtctcgt
    12901 tgcggtagaa gccgccgatg tcgaacttcc agcccgcacc gagggggccg ccgacccagc
    12961 cgtcgaagcg attgaggttg gaggtggggc catattcgta ttttatgagc ccactgaact
    13021 cgtcagtcgg cttgcgggtg atgaagttca ccacgccacc cggcgcattc gacgtgaaga
    13081 tcgacgacgg gccaccgcgc acgacctcga cgcgatcgat cgattcgtcg atgcgaaagg
    13141 cctggtcggc attgagatag ccgagtgccg gatcatgctg aacgggcagc ccgtcctctt
    13201 cgaggttgat cgaaccgaag ccgtcgacgg gaatgccgcg ggcccgaatg ttgccgctgc
    13261 cttcgccgcc cgacgcctcg acccagaagc ccggcaccga cttcagcgcc tcggtgacac
    13321 tggaaggcgc gcgggtgcgc agggcgtctg cggtcagcgt ggtgatggcg tagctggtct
    13381 gtgccttggt gcggccgcca gcgccggcgc ggccggtgac gacgatcgaa ttgtcaccgg
    13441 ccgagccggc ctcagccagc gaagaggggg gcgtggcgtc cgacgttgta ggctcctcgg
    13501 cgcgcaggct gatcgtctgg ccggtgcgcg cgccaacgat caggccggtg cccgaaagca
    13561 gctgccgcag accggcgtcg acatccatcg tgcccttgac ggcctgcgtg gtgcggccct
    13621 tgagatgagc ggctggggca acgatctgga cgcccgcctg cttcgcgaac gcagggatgg
    13681 ccgtcaccgc ggcctgtgcc ggtacgtcga agcttcgtgt ctgtgctgag gcaggctgcg
    13741 ccgcgcacgc caacgcgatc gccgaaacgg cgaccagtcc ctgatatcgc atttttagtt
    13801 tcccgctttc gtgcgatccg caatcggacc gttcaggggg tagatgcagg cggcgacgat
    13861 ctccgccacc gctgtcagaa ttttttatcg ccgccgttcg gacttcaggt gcaccttgcc
    13921 gctgtcttct gcgacatggg cgtcgaagct gacggcgatc gcctgggcga agccgcgggg
    13981 atcgcttgct gcgaacaggc ccgagatggg ctgtcgggcg agcgccgcat cgtcaatgac
    14041 gatggcaggg ccgccatagc ggcggaagcg ctgagcggcc tgggccagcg tctcgccctc
    14101 gaaggcgagc atcccgtcgc gccatgacag cgagcgggtg agatcgtcag gtgtgagtgc
    14161 gtgcgcgacg cccccggcat ccagtcggct gcccggaagg acgattgcct gcgcgttggc
    14221 gccgagctcg agcggcgtgg cgcttccttg cccgatcgtc cggctgagcg cgacccggcc
    14281 ttcgctcacc accaccgaaa ccggctgacc ggggaggttg cggacggaaa aggcagtgcc
    14341 gacggcgcga accagcagat cacgggcctc gaccacgaag ggccgtgcgg aatcgtgcgc
    14401 cacttcgaag aaggcgacac cggaaagcag gcgaacgtcg cggcgctctt tgctgaacgc
    14461 ggtttcgacc acgctgtcgg tgtcgagcgt gacgaccgag ccttcaccca gcggcacccg
    14521 gcgaatctca cctgtagcgg tcttcagtcg ccggccgcga tcgagcaagc gcgggatggt
    14581 cacgccggcg accagcgatg cggcaatcgc gccgccgccg agcatggcgg tgcgtcgatc
    14641 gattcgctcc cagaaggaag gttcgggcgg atcctgcgtg gcggccgggc cgagcgagcg
    14701 gacacgatcc gcgtgcgtcc agacggcgcg ggcgcgcgca agggcgccga tccggcggct
    14761 gtcgcctgcc agccattgat ccagtgcggc ccgttcttcg ctcgtcagcc cgcgatcgag
    14821 ccgcgccgtc caaacggcag cggtctcgtc aatattctgc gcgggttcgc gcccggtcgt
    14881 cggcaaacgg tcgtccttca tctctcggca tggatgcatc agacgacgct tttccgccac
    14941 ggccgaattg ctgcatcagc aattttatgc cgcgcacgat atgtttttcc acggtatttt
    15001 cggaaagtcc aagcacttgg gcgacctcgc gctgcgacag gccctcgatc cggcggagct
    15061 ggaaggcgcg gcgcgtctgc ttcggcatgg cggcgatagc gcgcgcgacg cgattgagtt
    15121 cgtcccgtcc gatcgcatgt tgctcggggc tgggaaggtc ggcggcgtga tcgagcgcct
    15181 ccagatccgc gaccgagccg atcgcgacga cccgtgagcg ccgcagatcc tgcagtagga
    15241 tcgatttggc aacctggaaa gcatacgcgc gtggtgcgcg gatcgaggcc acctccgccc
    15301 gggtggcaag caccgcgtag gtctcctgaa cgaggtcgtc gacgcgatcc tcgtcgagga
    15361 acgcgaaacg agccagccag tcccgcagcg aacgctcgtg cggcaggaca ttggtggcga
    15421 gccacagact tcggtcatcg ggagcatgag ccatgccccc tcattccggc gaactgttac
    15481 atttccaaag cagtcggcag attttctcag cttcaggcgg ttcgctcggg aacggccgcg
    15541 gcgtcctcga gaatggtcgc atcgggcgcg acggcttccc aggggaagac gaaccagtcc
    15601 ttggtcacgg cgcggtcgat cacgcgcgcc gcatattcga cccgcgccaa cgagcggata
    15661 ttgtcgatca gcgttgcaaa gcggatgttc gactgtacgg ctccggcttc cgccagcacg
    15721 gcccggaggt ggacgatggt ccgcccggtg tcattgatgt cgtcgacgaa caggaggtgc
    15781 tccccctgct gggtgcggcg cgccagtttg agcaacggtt ccgccgcaaa atcaggaacc
    15841 tgcgaggagt agtcgacaga caatgtcggc aggccgatgg cgtgggacag gaacaccgcc
    15901 ggcaccagcc cgccgcgtcc gatcccgatc aggaagctgg gccgccagtc cgcttgggta
    15961 agctgtccgg cgagatcatg gacggcatcg acgaattcgt gctgcgggat cggcgtaagc
    16021 tcaggcatgg gcacgttcct tcgcgggagc cgaggctgcg agataggcgt cgagcccggc
    16081 gagatgctgc gtcagcgccg cctcgtcgag gaacgatccc agcaggctgt tgcgcgccag
    16141 catggccagc cgatcccggc cgatcccgcg ccccttcgcg acggcgcgat aattatctgc
    16201 gatatatccg ccgaaatagg ccggatcgtc ggaattgatc gtggcacgca ggcccgcatc
    16261 cagcatccgg tcgatcgggt ggccggcgat gtcgtccacc acgcagagct tgacgttgga
    16321 cagcggacag accgtcagcg tcattgcctc acgggcgagg cgggcgacca gacgcggatc
    16381 gtcgagcgaa cgattgccat ggtcgagccg atcgacgccg agcaggtcga gcgcttccca
    16441 tacataggcg gcggggccct cttcgccggc atgggcgacg cgcttgaggc tcatgtcggc
    16501 tgcacgggcg aagacgcggg cgaatttgga cggcgggtgg cccagctcgg aggagtcgag
    16561 gccgacgccc gcgatccgat cgagccaagg ctccgcttct gctagcgtgg ccaaagcgtc
    16621 ggcctcatcg aggtgtcgca ggaagcagag gatcagttgc gaggagaggc catggcgtgc
    16681 ctgcgcggcg gccatcccgg caaggagccc ctcggcgacg acgccgaacg ggatgccgcg
    16741 ggcggtgtgg gtttgcggat cgaagaaaat ctcggcatgg accacgccgt cggccgcggc
    16801 acggtcgaaa taagcctcgg cgagatcctg gaaatcccgc tcggtacgca gcacgtcggc
    16861 gccggcataa tagatgtcga ggaaatcctg gaggcgcgag aactggtagg cggcgcgaac
    16921 ctcctcgacg ctcgtaaagg ggatcgacag cttgttgcgc tctgcgagcg cgaacatgag
    16981 ctcgggttcg aggctgcctt cgatgtggag atggagttcg gccttgggca ggccggtgat
    17041 gaaggcgtcc agatcggtca tgcgtcgaac tctggccggg gcagtggatt cacgcaagcc
    17101 ggatggcgac acatgttcaa ttgaacaggc ggttggcgcg caggcggtcc actgccagat
    17161 cgacgaagct gcgaaccttc gccgcgcccg acgcccttcc ggatggacga catggatggg
    17221 gaggggggct tcctcatact cggtcagcac ggtctgtagc ttgccagcag cgccggcccg
    17281 acctggcaga acaacaggcg tgggtggacg gtcaagccgg tccgcttgtt ccgcccgaag
    17341 tgccattcgg gcaaggccca tgcgccggtt gggcgacgat ccagtagtcg cgcagttcgg
    17401 cggctagcca ctcacgctcg gaaagatcgg cctcgacgac gcgaagcagg ccgtggccgc
    17461 ggtcgatcac ctcatccgcg tcgcgcttgg cgccgaatac ctgatgagac gcgccacgca
    17521 cggctcatgc gccgcttggc cggccgcgac ggaaagccag cgccgcaccg cgggctggat
    17581 aatgatcctt gccagctcgt tgcagaagtc gggtttaagg caagtcacta ttccgtattt
    17641 cggaatagac cgatgggtcg aacagggcac tgcgtgtgct gccgcgcgtc tgcggggggg
    17701 agggagtgtg gcccgcccgg cgcgacccga ggctagctcg atttcgcact tctctggtag
    17761 gggcgtcggc atgcagggac gagatatcat gacttaccgg cttctgatcg atggcgcgct
    17821 ggtcgatggc gctgtgacgg tggaggtggt gaatcctgcg accggacgcc ccttcgccat
    17881 cgcgccgcgc gctgatgtcg tccagctcga gcgggcggtc gcggcggcga agcgcgcctt
    17941 tcccacatgg tcacgcacgc ggtgggctga gcgtcaggcg ctgctcgcgg cgatcgccga
    18001 cgtgcttgcc gataaggggg aggagttcgc ccggctgctc acccaggagc agggcaaaac
    18061 cctcacggag gcgcgtggcg agatggaggg cacgatcgcg gctttccgtt actttgcgac
    18121 gctggaactc tcgccccgcg tcctgcgcga cacggcgacc gaccgcatcg tcgaatatcg
    18181 gcatccgttg ggggtggtcg cggcgatcgt gccatggaac tatccgctgc tgatgctggc
    18241 gctgaagatg gcgcccgcgt tgcttgcagg gaacacgctg atcgccaagc ctgcaccgac
    18301 cacgccactc gccacgctgc ggctcggcga gatcatggcg gagatggtcc cggccggtgt
    18361 gttccagatc ctcattgacg agaatgatct gggtccgctg ctcgcagcgc atccggatat
    18421 cgcccatgtc agcctcacgg gatcgacagc aacgggacgc atggtgatgg gagcggcttc
    18481 gggcacgctc aagcggcttg tgctggagct gggcggtaac gacgccgcgc tgctcctcga
    18541 cgatgccgac atcgcatccg tggcgccgaa gatcttcgag agcgcgatgg cgaatgcggg
    18601 tcaggtctgc ttcgccatca agcgcgttta tgcaccgcgc gcactcgtgg gcccgctctg
    18661 cgacgccctg actgagctgg cgcgagcggc tgtgatcggt gacgggatga tcgaggggac
    18721 caccatgggc ccggtccaca atcgcgccca gttcgacaag cttgccgcgt tgctcgacaa
    18781 gacgcgcggc gaggggcgga tcgtggccgg cggcgaggta ttgccgggcg aaggctattt
    18841 catcgcgccc accgtcgtcc gcgatctggg cgatgatgcg ctggtggtgc gcgaggagca
    18901 gttcggcccg atcctgccgg tgctcggcta tgacaatctc gacgacgcaa tcgcccggat
    18961 caacgcgagc gaatatggcc tcggcggatc agtgtggaca cgcgacgtcg agcgcgggat
    19021 cgcggtggcg gcgtggatcg agagcggcac cgtttgggtc aacaagccga tcgacctgcc
    19081 gttcgatatt cccgtcggcg gcgtcaagca gtcgggcatg ggtcgccagc aggggatggc
    19141 ggggctggaa gagttcaccc agccccgcat cgtcaacgcc gcactcggct aatggtccct
    19201 caattctggg cgcgaagttc tgcgacctgc ttgggcgtga tggtcgacgg cttcgaaccg
    19261 aaacgagcgg tgacatagtt ggcgaccgac gcaatctccc gatccgtata ggcgacggcg
    19321 aagctcggca tgtagggacg gccgccattg ggcgtccccg cgccgttcag gatcatctgc
    19381 gcgacgttga tcgcggtaac gtcgttcacc gcgcgattgc cggtgagctg cgcctcctgg
    19441 atcagcgcgc cgttgccgtt ccaggcgtgg cagctcgcgc aattgccttc gaacagccgc
    19501 ttgcccacgg gattgtcggt cgggcctgcc ttgggggcta ccggggccgg gccggccagc
    19561 tggggtagct ggctgctgtg gatcggcggg acgctctgaa gataggtgac catcgcgccg
    19621 atatcgccgg gcgtcagctt gctcagactc agatccaccg cctcgcgcat cggccccgac
    19681 gcggtgcccc ggccgagcgc atgccccttg gccagatagg cggcgagatc ctgcgctgac
    19741 cacgcgccgg tgccgctcac acgatccggc gtgatgttat aggcattcca gccttcggcc
    19801 tgaccaccgg cgaacttctg ccgggtgttc atggcttgca gcagattgcg gggagtatgg
    19861 cactcgccgc agtgcgatgc cgcctcgacc agataggcgc cgcggttcca ttgcggactc
    19921 tgctcgggaa tgggctggaa gcgcttgtca ctgttgaata aagttgacca gatggccatc
    19981 agccagcgct gattgtaggg aaagccaaag gtgttcggtt tcgcttcctg ccgcacgggc
    20041 ttcagcgaga agagataggc cttgatcgcc agcacgtcct gatcggtcag catcgtgtag
    20101 ctggtatagg gaaaggcggg gtagagccgc gtgccgtccg gtgcgatgcc tttgtggacg
    20161 gccttcagga aatccgtgtc gctccatttg ccgatgccgg tatccgggtc cggtgtaatg
    20221 ttgggagtgt agagcgtccc gaacggcagc gtgaaaggac gtccgccggc aaagggcgtt
    20281 cctcccttgg tggtgtggca gccctggcag tcggccgcgt cggtcagata tttgccgcgc
    20341 gtcagcgggt ctgcgttcgc gagctcggcg ggtacgccga ctggcgagga gccccgatat
    20401 tgggcgagat cgacggggtt gcctttggcg aaggcagtcg gccccggttg cccgaccatc
    20461 cagccgagta tgcccagcag caccacgccg accacgacga tgatgccgcc gatccagaga
    20521 aggacgccct tcatgccttg gctcccttcg ccagcagcgc ccggttgatc ggcatccgtt
    20581 tgagctgaac gccggtcgcg tcgaacaccg cgtttgcgat ggccggctgc actacgacgg
    20641 tcccgggctc gccgatgccg ccgggctttt cctcgctggg cacgaaatag gtttcgatcc
    20701 tgggcatctc gtcgatgcgc agcacgcgat agtcgtggaa attgctctgc agcacacggc
    20761 catgatcgat ggtgatctca ccgtgcagga tggcgctcat cccgaagatc gtgccgccct
    20821 gcacctgggc ttccagcgtg ttgggattga tcatcttgcc gacgtcggcc gcacagacga
    20881 tgcgcttgat ctgcacgtcg ccgtcatcgg gcaccaccac ctcggccacg caggcgagga
    20941 agctgccgaa gccggcgagt agcgcgatgc cgcgcccggc ccgcccgcca ccactcggcg
    21001 ccgcgagggg ctggccccat ccggccttct ccgccgcaag gttcagcact gcgagcgcgc
    21061 gtggcgattt cttcagcatc ccacgccgga actcgactgg atcgaccccg gtctgatgcg
    21121 cgatatgatt gatgaagcgc tcggccgaga aaatgttgct gttggggccg acgcctcgcc
    21181 agaaagcgac cggcacctga ggtgtctcgt ggcggatata ttcgatcagg atatggtcga
    21241 aatcataggg ctgctcgatt gcgccatcga ccgcgtcggg gtcgataccc ttttggaacg
    21301 cgggggggag ccagcgtgcc ataatggagg gtccggtgac gcgatgatgc cacgcggtga
    21361 tccggccatt ctcgacgcgc gcgcgcagcc ggtcatggta gatcggccgg aagagctcct
    21421 gctgaatatc ctcctcgcgc gaccagacga tcttcaccgg gccttcgaca tgctgcgcga
    21481 tccgggcgac cttgacgaca ccgtcgactt ccagccgccg gccgaagccg ccaccgatca
    21541 ggtgattatt gatcgtcacc tgctcaggct tgaggccgag cgccctggcg gcgccttcct
    21601 gcgccatgac gggcgcctgc gtgccgaccc agatctcgca actgtcgccc ttgaagtggg
    21661 cggtgccgtt gatcggctcc atcggcgcat gggcgagata gggaagctcg aactcatcct
    21721 cgtagaccgt gccgtccttc agatgggcgg gcgcgtcgcc gaccttctgc gcggtgacgc
    21781 cggagccgtt cgccgccttg gcctgcgccg cgtacaggtc ttcctgcatg gcatgggcgt
    21841 tggggccata gtcccaatcg atcgcgagcg cggcgaggcc ctgtttggcc gcccacatgt
    21901 gatcgccgac caccgcgacc agatcgtcca gcaccaccac ctgacggaca ccgggcaccg
    21961 ccctcgcctg actgtcgtcg acatggcgaa ccttgccgcc gaagacgggg caggcggcca
    22021 gcgtgccgaa tttcatgccc ggcagcatca cgtcgatgcc gtattgaacc ttgccgttca
    22081 ccttgtcggg cgtgtcgatc cgcttcaacg gcttgccgat caggcggaaa ctcttgggat
    22141 ccttgagtat cggctccttg ggcgccggga tggcagccgc ccggtcggcc agcctaccat
    22201 agggcgctga acggccgctc gcatcatgat ggacgacgcc gctctcggtg cggaggctgg
    22261 ccggatcgac gttccagccg gctgccgccg cccccaccag catcgcccgc gcggtggcgc
    22321 ctgccttgcg gagcggcagg taccagccca tcgtgctggt cgatccgccc gtgatctgcg
    22381 cgacgaagat cgggttgcca taatccttct gatccggcgg ggcgtgctcg accgtcacct
    22441 gcgccagatc gacgtccaac tcctcggcga tcagcgtcgc catcatcgtg taggtgccct
    22501 gacccatctc cacagctggc atgatcatgg tgatcgcgcc gtcgcgggcg atgcggacga
    22561 aggcgttgaa cgtcgcgccg ccctgtggac gggggtcctt cgcctcggca gagcggccgc
    22621 cgatcagttc gatgccgagc agcagcccgc cgccgactgc ggcgccggcg cccagcaacg
    22681 tgcggcgcga caggccgcgc gatcccccgg tggaggcagg cgagggatca ggccgatcgt
    22741 gcagcatgct tgatgccctc ccggatgcga acataggtgc cgcagcggca gatattgccg
    22801 gccatggcgg tatcgatatc ggcgtcgtca ggcgacggat tgctggcgag cagcgctgcg
    22861 gccgacatga tctgtcccga ctggcaatag ccgcactgga cgacctcgag atcgagccat
    22921 gccttctgga tcttcgcgcc tgcgggtgtg tcgccgatcg cctcgatggt ggtgatcgcc
    22981 cggtctccga tcacgccgac cggtagctgg caggagcgga ctggactgcc gtcgagatgg
    23041 acagtgcatg caccgcattg cgcgataccg cagccgaact tggtgccggt cagcccgatg
    23101 acatcgcgca gcacccagag cagcggcatg tcggctggaa cgtcgacggt tcgttcctgt
    23161 ccattgatct tcaagatggt cacgaccgga tccttgctaa gggctgggat ggcgcttgaa
    23221 cgagggcgtg gagaaagaga ctccgtgatc gtatcgttgg cgcaccatat cttgctctgc
    23281 gatcgatgga agcagaagtg ctgttcaaac ggtcggggca ggaaggatca tcatcgatcg
    23341 agagcgtgct tgcgaccgtt cctcgtgaac catagcatcc cagcagcccc tcgaacctgg
    23401 agaaagagga tgaccggctt cgccttccag tacggacccc gcctgcttgc cgggccgggt
    23461 gtttcgacgc ggatcggcga actggtttcc gcccgacacg tgctggtcgt gacggacggc
    23521 caagtgcgtg cgctggccct cgccgacgcg atgctcgacg ttctgagatg cgaagggcgc
    23581 agcgtgacca tcttcgatgc ggtcgaggcc gatccttccc ggtcgacgct ggaggctgca
    23641 gtcgaagtcg ggtggggcgc ggaggccgtc atcggctttg gcgggggcag tccgatggat
    23701 gtcgccaagc tcacggccta cctgctgggc tcgggagacg atctcgatgc gatctggggc
    23761 gtgggcaatg ccgcgggtac gggcttgccg ctggttctgg ttcccaccac cgcgggcacc
    23821 ggatcggagg cgaccccagt tgcgatcatt tctctaccgg ggggcgagaa gcgtggcgtg
    23881 tcgtcgtcgg ctctgatcgc gcatcatgcg gttctcgatc cgaccttgac gctcggcctc
    23941 ccgcggcatg tcacggcagc gacgggtatc gacgcgatgg tccatgcaat cgaggcctat
    24001 acctccgctc acctcaagaa cccggtatcg gacatgctgg cgaaggaggc gctggggttg
    24061 ctatccagcc atctgctgcg tgcgtgcgag gtgcccgatg atatcgacgc gcggggcgcg
    24121 atgctgctgg gcgcgcacct tgccggtgtc gccttctcca atgcgccggt ggcaggggtg
    24181 catgcgctgg cgtatccgtt gggcgggcgc ttccacgtgc cgcacggact gtccaacgcg
    24241 ctgatgcttc ctcatgtgct cggccacaac atgcaggcgg caatgccgct ttatgcggag
    24301 cttgcgccga tcgtagaccc atcccttgct cttctcggtg ggcaggctcg ggctcagggg
    24361 cttgtcgagg cattgcgcgg gctgtcgatg gcggcgggca tgccccagcg gctgcgcgaa
    24421 gtcggcatcg gtgttgagca tctcgaaatg ctggccgccg acgcgatgca gcaggaaagg
    24481 ctgttacgca acaatccatg ccccatcgag ctggccgacg cgcagcgtct ctacgaggca
    24541 gccctgtgaa ccgcgagccg ttgcgccccc gctctgccta ccgccactgg actcgcatca
    24601 gcacgcgctg gcatgacaat gacgtatacg gccacgtcaa caacacggtc tattatggct
    24661 ggttcgacac cgccgtgaac gcctggctgg tggaggcggg gctgctcgat gtcgagcggg
    24721 gcgatccgat cggcctggtc gccgaaaccg gctgtcgcta tgccgcaccg gttgcgttcc
    24781 cgcagcctgt tgaggtcggc ctcacgctgg caaggctcgg ctcgtcgagc gtgacctatc
    24841 atctgggtgt atttgccgag gggaccgacc atgcggccgc cgaggggcat ttcacccatg
    24901 tctatgtcga gcgggagagc cgccggcccg cgcccttgcc cgaggagtgg cgtcacctca
    24961 tcgagacgct ggcttagccg ttgtccgcga tggtggagcg ccggatcctg tcagtcgggg
    25021 ccaaagaaga ggcccggcca ttggggtgcc gggcctcttt ctgttttggc agtttaagcg
    25081 gccacactcc gcgccatgta acggtctgcc ggctgggcat gcgagaggtt cggcttgagg
    25141 atctgccgag cggcttcata cgcattccaa tcggccgcgt ccggaagcga cgggatggtg
    25201 acaagctcgc ccatgtcgag cccgacaagc gatgcgtcga ccatgtcggt cgccatcatc
    25261 acgatctcgc ccgggagata atggatcggc gttccggcga tatcccagaa gtcggtcgcg
    25321 gtggcgccgg gcagaacagc ctggacgcgc acgcccttgt cggcgagctc atgatgcaac
    25381 gactgggtga gcgcgaggac aaacgccttg gagccaccgt agacaccgtt gagcacctcc
    25441 ggcccgatcg cgacgatcga cgcgatgttg atgatcgtgc caccgccgcg cgagacgaaa
    25501 cccggtgcag ccgcataggt gaggcgggtg agtgcggtca cgttgagcgt gatcatggcc
    25561 tccatctcgt cgacatcggc cgagagaagc ggggcggtag cgccgacgcc ggcgttgttg
    25621 accagaacac caatccgctc gtccgtcttc aggagcgttt cgatccgagc gaggtcgtcc
    25681 ttgcggccga gatcggcggc ggcgacctcg acgatgcgcc cggtctcctc tctcagcttt
    25741 ctcgccagcg cttccaactt ttcctggttg cgggcgacga ggatcaggtc gtgcccacgc
    25801 cgtgccagcc ggtcggcgta aatggcgcca atgcccgtcg aggcgccggt gatgagagcg
    25861 gttccgtggt tcggcttgac catcatgcat ctccttcaac atcgccagca gggggagggc
    25921 tggcattcgc gcggaggtta ggctcttcag tgtctgactg aaatgtcgta tatcacacta
    25981 attaggacat ggagggctgg atgcgaatag ggttcgtgat cttcccaggg ttccagatga
    26041 tgtcgctggc ggcggcttcg gtgtttgagt tcgcaaacgt ccagagcggc gaggcactct
    26101 acgatatccg gatcctgtcg gagacgggcg gccctgttcg aagttcgacg ggtatcatcg
    26161 tggacagcac tccgctgggc gcggcacggc tcgacacgat gctcgtctgc ggtggggtag
    26221 gtatccccga tgcatcaccg gcgctgctgg cgttcatacg gggctcgacc agcgcggtcc
    26281 ggcggctcgc gtcgatttgc acgggggctt tcgtcctcgc gcaagcgggg cttctcgacg
    26341 ggcgcaaggc gaccacccac tggctcctcg cgcgtgaact ccaggcggga tttcccgggg
    26401 tgagggtgga ggacgaccgg atcttcatcc gcgatggcag catctggaca tcggcgggca
    26461 tgacggcggg tgtcgacctg gcgctcgccc tgctcgaagc ggatcagggc gaggaactgg
    26521 cgcgcgcggt tgcacgcaag ctggtgctct accatcgtcg ggcgggcggc cagtcgcaat
    26581 tctccacctt gctggagctc gagccgaaat ccgatcgcat ccagcacgcg ctggcctacg
    26641 cccggagcca tctgaaggct cccctttcgg tagaagagtt ggccgacgtg gccaatctca
    26701 gcccgcgcca gttcagccgc gcgttccgca gcgagaccgg gcagtcgccg gcgaaggctg
    26761 tcgagcatct ccgcgtcgag gcggcgaggg tgatgatgga gcagggccgg catccggtgg
    26821 atgtcgtcgc gcgcgagacg ggctttgccg atcgggagcg gatgcgccgc gctttcctcc
    26881 gggcctttgg ccaaccgcca caggctgtcc ggcgcaatgc gcggggtgga atggcgccat
    26941 cgcccgacgc ggtcgcggat agcgtccatt ccatgtaata ttcgagaccg gcttcgatcg
    27001 agatcgcctt gaacggcttg aggttgggct gtcgcgcggc ttccatcttt ggcgcagggg
    27061 agaagtggcg gagcggctga gggaggccgg tgcatgacct acgcaatcga tcggctggtg
    27121 ttcgtacctg acgacgtcga tctccagcgt tcaccactgg cggggcagat cccggctgag
    27181 acctatgtgc tcggtgcgtt caatccaggg ctcgcccgtc tcgcgaatgg caatctgctg
    27241 ttgatggttc gtgtcgccga agcgcttcgt acccccaact tcgacgggca tgtccacgcg
    27301 atccggtggg acggagagca ggggcgctat acgctcgacg cctggccgct cgacctcgct
    27361 gacacggcgg atccgcgcaa gttcttcctg cgtggtggtg gatggccgat catcgccctg
    27421 acctcgctct cgtggctttt gccggtcgag ttcagtcctg atgggctgga gcgtgtcgca
    27481 atccactatg atcgggctat cgcgccgcaa tacagcttcc agtgctacgg tatcgaagac
    27541 gcgcggatca gccggttcga cctgcaatat ctgatgacaa cctgctcggt cagccccgag
    27601 cggcaggcca cgacgctcta cagttctacg gacgctgtcg actggaggtt cgaagacatc
    27661 gtgctcgatc atcagaacaa ggacatgctg atcttcgaag gccggatcgg aggccagtac
    27721 tgggcgcaga cgcggccgct cggcgacgtg tatttcgcct atccgccggg cagcgtgtgg
    27781 cggtccgggc cttcgatcaa tctcgcaacc tcacccgacg cgcatcactg gaagccctgg
    27841 agagaacccg gcatccgacc ccgtaccgac agcctcgcaa ccgcgcgcat cggcggcggc
    27901 gccccaccga tccttacccg cgacggttgg ctgagcctct ggcatggcgt cgagccgcag
    27961 gagatcatcg ggatctaccg cacctactgg tcgatcatgg accgcgacga tccgagccgg
    28021 atcgtgcgaa gcggttccgc gccgctgatc gagcctgccc ccgaactgac ccgcccgatc
    28081 gagcatcaga tgtatctcag tgatgtcgtg ttcacgacgg gcatcgccga tggcggggag
    28141 cattatatcg tcgcgtctgg tgaggcagat ctggcgtgcc gcatcaccca cgtcccgaag
    28201 tctgcgtttg gactggactg atacgcggat cgcactcgcc ggctctttcg ggcggagcat
    28261 gtcgaaccgc gcaagccatg ctacggcaaa ctccggaccg acggccgcgc gaaaacctcg
    28321 tcgggcggcc cctccgcgac aaccctgcct tgatccaggc agacgacgtg atcgacatcg
    28381 tacagcgttt ccgtgcgatg ggtgatcagc aaccgtgtgc ggtcggcgaa gcggcggtcg
    28441 attccatgtc ggatgccgtc ctccagcctc ttgtcgaggg cgctcatcgc ttcgtccagg
    28501 atcaggaagt cggggtcgcg caggatcgcc cgcgccagcc cgagccgttg tctctggcca
    28561 cctgagaaac gcaatccctg ctggccgatc caggtggagt atccgtccgg aagcgcgttg
    28621 atcgtgtcgg cgatgcccgc cacccgaagc gcggagcgga cgtcgtcgtc gctcgccgcg
    28681 ccgttcgcca tgcggatatt gtcgatcacc gtcccttcga tcagatcgac atcctggcca
    28741 gcgatcgcga gcagatcgag ccagtcggtc cgccggatat cctcaagcgg caccccgtcg
    28801 atcaggatcg ttccagccgt tggttggtaa agccggagca gcaggttgac gatcgtcgtc
    28861 ttgcctgcgc cgctggcgcc gaccagcgcg gtcgtcttgc ctgcggggat ctcgaacgag
    28921 atgtcggtga gcgccgcatc cccggcgttg tcatagagga aaccgacatt ctcgaagcgg
    28981 atgttcgatg ccagggtgcg gatcgggcgg tggccgggtg agggataatc cttgtcatcg
    29041 gtcgcgagca tcgcccgcac ggaggcaagc cgtggctcga tctgcgcgag cccaagcaga
    29101 ttgctttcca attcgcgcag atgcggctgc aaccggtaga gcagtgcgat ggcggccaag
    29161 gtgatgggaa agccggtctg gcctctgtcg gcgagcgcga tgatcacgca cagcacgccg
    29221 atatagccga gttcggtcag cggatggagc tgggccatca ggcgtgaaag ggcgatggct
    29281 gtccgtcggg cgtccgccga tgcgtgttcg aagcgttcct gatggagcgc ctcctgtcca
    29341 taggcgcgaa tcgttcgcat cccctgcagc gtcatcagca tgtgctcgcc gaggagctgg
    29401 tgaacatatt tgacgcggat gccaagctgg cgggcagacc gggacaaata acgcagcccg
    29461 atcgagatca gcagcgatcc gaggacggcg acgagtgtga tccgccacga caggctgatc
    29521 agtgcggccg tgaagaccag gatcgagcag atattgacga caattctcgt caggctgctg
    29581 tgcgcgcccg agatcagcca ggtttcggtt cccagtactt ccatcagatg ggcctgctca
    29641 tgccgctgga tgaaggggta ggcgacacgg agatattgtc gatgtacgcg gttgcgcaca
    29701 tcctcgctga tctgctcgct gatgcgagcg ctcagcaggc cgtgcaggaa cgacagcccg
    29761 ccccgcgcga tgatcagcag caggatcacc gcggcgatct cggtcgaact ggcgaaccat
    29821 cgggtggctt cacgcaacgg cccgcccagc aggccctgca ccggcacggc ctgatcgatc
    29881 tgccccatgg cggagtagaa gaacaccacc accagggtga tccccaatgt ctccgccagt
    29941 gaggagatca ggccgagcac cacgagtgtc agcgttgtcc atcgcggcac cggcgcggcg
    30001 gcgaatagac ctagcaggcc cctgaaggtg ccccgtcggc tctccggtgc gaccgttgct
    30061 gcggggcgat cggcggcggt caagcgcatt ggccgggttc gttgatgaag agggcgggcg
    30121 agcgtcgttc aggtgggcac agcaggcggc gcagcctcgc ctgcagcctc cagcgcactg
    30181 cgttcagcaa cgtcagcggc aggcggaaga acagcaccga aacggcgatc cgcggctcct
    30241 ggcggacgag gagatcgatc agcgagagtg cgtacgcaag cctgcctgtg gtcaacgcct
    30301 tgaacaacaa ccacgcgcca tagttgcgca atcctacgtc tagcgcggcg tggcgatccg
    30361 ggtgacgctc ctccatctca tctttcacca gcatccagga tcgaagcatg cggggaagat
    30421 tgctggacat gttgtcgggc agatagcggt agccgacgag ataatcgggt atcactgcaa
    30481 agccgtgccg ttctgcagcc cggcagtaga acagcagatc ctcgcacccc tgcgcgtcgg
    30541 cggtgcggag cccgctctcg aagccgcgcg cgtcaatcag cgtctggcgg cggaccagtg
    30601 ctgcgctgcc gttgccgatg aagttacctg aaaatatgtc gtcgagaacg tcgccctcgt
    30661 ggagagggca gccgccatgg tggagcacgc gtccctcgcc atcaatcagt tgataccaag
    30721 tgtagacaag gccgacgcgg tcaccgccag ccagcagcgc gtcgagctgg cgttcgatct
    30781 tggtcggtgc ccacaggtcg tccgcatcga
//
LOCUS       DATGEM010000051         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_208004, whole genome shotgun sequence.
ACCESSION   DATGEM010000051 DATGEM010000000
VERSION     DATGEM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208004"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VKQ27_01480"
     CDS             <1..1145
                     /locus_tag="VKQ27_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY87631.1"
                     /translation="PGPPRDWSDHTASAMTLLNGSTPHDSFRNKIVFIGSSAPELGGL
                     RETAASLTTPSVWLQAEAVATMLQDRIAVRPGSATKVELAAALALGPLAILFGLWFVP
                     ALATGLTLLLCVAWSGAALTAIGGFHLLIDPLGPPALALIGFIAAMLCRFARDEWRAR
                     MLRLSFEQHLAPDVVRRIAADPGAIRLRGELRVITALFTDIEGFTSMTERAGPTELVA
                     LLDTYFEATTRVITDHGGMIDKIVGDAIHAIFNAPFTLEDHPRRAVESALALLNASQT
                     IRGSPLGQKLQLGRTRIGIETGLAIVGDVGGRRKLDYTAHGDAVNAAARLEAANKEFG
                     SSICIGPGTAGHLDPATLHPIGTFVPRGQSREVTVYTVAIPPSAAP"
     gene            complement(1160..1774)
                     /locus_tag="VKQ27_01485"
     CDS             complement(1160..1774)
                     /locus_tag="VKQ27_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HLY87632.1"
                     /translation="MRHTLRRRGVLAGVACVLATPVVRVCAAPQRAGEVKTIHGDCFA
                     RLGVARRTLVPNSDVFVGDSVGTGPASSLAMLLGEATTVRLGPEAQLRIDRFLMKAGG
                     VLTLDKGAVLYDHDAKDGPDNAVVRSPFGLIAVRGTRFFAGPSNGVFGVFVERGEVLV
                     VGVSTAVTVTAGMGTNIATPGAEPTLPTVWGRPRIAAAMGQFGQ"
     gene            complement(1816..3270)
                     /locus_tag="VKQ27_01490"
     CDS             complement(1816..3270)
                     /locus_tag="VKQ27_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HLY87633.1"
                     /translation="MSLRPASPRIRTSGRTRLTAVIVACALFMQNLDSTVIATALPTM
                     AKAFGADPVHMNVALTSYLLSLAVFIPASGWVADRFGTRTVFRAAIAVFTVGSVLCGR
                     ADSLAFLVGSRVLQGIGGAMMVPVGRLVLLRTAPKNELVAAMAWLTMPALLGPVLGPP
                     VGGFIVTYFNWRWIFDINVPIGVLGIVLVSLFVEDVREPPRGRFDMIGLVLCGVALSG
                     VMFGLETAGRGVVPLATTELMVGVGLVAVVGYVLHAWRHPAPLLDLSLMRLPCFGVAF
                     SAMMLFRTGIGAIPFLLPLMLQVGFGDSAVQSGLITFASSAGALVMKPATQYALRWFG
                     FRDTLVWNGLVSAAMIAICAAFRPTWPAAAIYAALLIGGFFRSLQFTAYNTLAYGDVP
                     RSRMSSATSLYVTGQQLAATIGISTGAASLEISRAMSGHLTAEPIDFSHAFLVVALLT
                     LAAAPIALLMPRNAGDELTGRAPVVAEPVRRPAE"
     gene            3324..3899
                     /locus_tag="VKQ27_01495"
     CDS             3324..3899
                     /locus_tag="VKQ27_01495"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLY87634.1"
                     /translation="MSAQGFALFDTPIGPCGIVWSDTAVVGMQLPERNGPTARSRIQR
                     RHPTAREAPVPDHIQPVIGAITALLGGEKRDLADIPVDLTGLSSFQRRLYETLRTVPP
                     GATISYGELATRLGDGVTARDVGEAMGQNPVPIIVPCHRVLASGGKLGGFSAAGGVAT
                     KVRLLEIEGAPIGEAPTLFGDLPLAAKPGRR"
BASE COUNT          784 a         1447 c         1175 g          620 t
ORIGIN      
        1 ttcccggacc acccagggac tggtctgatc atactgcctc cgccatgacg ctattgaacg
       61 gatcaacgcc acacgactcg ttccggaaca agatcgtctt tatcggcagc agcgcgccgg
      121 aactcggcgg cttgcgagag acggccgcgt cattgaccac cccttccgtc tggctgcagg
      181 ccgaggcggt cgccacgatg ctgcaggacc gcattgccgt ccgcccaggt tccgcgacga
      241 aggtcgagtt agccgctgcg cttgcactcg gtccgctggc aatcttgttt ggattatggt
      301 tcgtccccgc gttggccacc ggtctgacac ttctgttgtg cgtagcctgg agcggcgcag
      361 ctttaactgc catcggcgga tttcatttgc tgatcgatcc tctcggccca ccagcgctcg
      421 ccctgattgg cttcatcgct gcaatgctat gccgctttgc ccgcgacgaa tggcgcgccc
      481 gcatgttgcg gctgagcttc gagcagcacc tcgcgccgga cgttgttcgc cgcatcgccg
      541 ctgatcccgg tgcgatccgg ctgcgcggag aattgcgagt gatcaccgcc cttttcaccg
      601 acatcgaagg cttcaccagc atgaccgagc gcgccggacc aaccgaactg gtcgcgctgc
      661 tcgacacgta tttcgaggct acaacgcggg taatcaccga ccacggtggc atgatcgaca
      721 agatcgtcgg agacgccatc catgcgatct tcaacgcccc tttcaccctg gaggatcatc
      781 cgcgccgtgc ggtggaaagc gcgctcgcgc tgctcaacgc atcgcagaca attcgaggct
      841 caccgcttgg ccagaaactc caactgggcc gaacccggat tggcattgaa accggcctgg
      901 cgatcgtcgg tgacgtggga ggcagacgca agctcgacta caccgcgcat ggtgacgccg
      961 tgaacgcggc agcacgtctc gaggccgcca acaaggagtt cggatcgagc atctgcatcg
     1021 gcccgggcac cgccggccat ttggatccgg ccacgctgca cccgattggc acgttcgtcc
     1081 cacgcggaca aagccgcgag gtcaccgtct atacagttgc catcccaccg tcagcagcgc
     1141 cttgacgtcc ttccgaccgc tattggccga attgccccat tgccgccgcg atgcgtgggc
     1201 gcccccatac tgtgggcagc gtaggttcgg cacccggcgt cgcgatattg gtacccatcc
     1261 ccgccgtcac ggtcacggcg gtcgaaacgc cgacaaccaa cacctcgcca cgctcgacga
     1321 agaccccgaa caccccattg ctcgggccgg cgaaaaaccg tgtgccccga actgcaatca
     1381 gtccgaatgg cgagcggacc accgcattgt ctggcccatc cttggcatcg tgatcgtaca
     1441 ggaccgcgcc tttatccaac gtcagaaccc cgcccgcctt catgagaaag cggtcgatgc
     1501 gcaattgtgc ttccgggccg agacggacag tcgttgcttc ccccaacagc atagcgagtg
     1561 atgacgcagg gccggtgccg accgaatcgc caacaaaaac atctgagttc ggcaccaaag
     1621 tgcgccgcgc tacgcccaat cgggcgaagc agtcgccatg gatcgttttg acctcgccag
     1681 cacgctgtgg cgccgcgcag actcgaacga caggcgtcgc caggacgcac gccacccccg
     1741 ccaatacccc gcgtcgtcgc aatgtgtgtc gcatgcgcag caacttacgc aggtcgggtc
     1801 aaggtaccaa ggaccctatt cggcagggcg ccgcactggc tccgctacca caggcgcccg
     1861 cccggtcaac tcgtcgcccg cgttgcgtgg catcagcaat gcgatcggcg ccgccgccag
     1921 cgtcaacagt gccaccacga ggaaggcgtg gctgaaatcg attggctccg ccgtgagatg
     1981 ccccgacatc gctcgcgaga tttccaggga cgccgcgccc gtcgagatcc caatcgtggc
     2041 ggcaagctgc tgtcccgtga catagaggct ggtcgccgaa ctcatccgcg accgcggcac
     2101 atccccataa gccagcgtgt tgtaggcggt gaactgcaac gaccggaaga acccgccgat
     2161 gagcagcgcg gcatagatcg ccgccgccgg ccacgtagga cggaacgccg cacagatcgc
     2221 gatcatcgcc gccgagacca aaccgttcca caccagcgtg tcgcggaagc cgaaccagcg
     2281 cagcgcgtat tgcgtcgccg gcttcatcac cagcgcgcct gccgaactgg cgaaggtgat
     2341 cagcccgctc tgcaccgcag aatcgccaaa ccccacctgc aacatcaaag gcagcaggaa
     2401 cgggatggcg ccaatgcctg tgcggaacag catcatcgcg gaaaatgcca cgccaaagca
     2461 cggcagccgc atcagcgaca gatccagcag cggcgccgga tgccgccaag catgcagtac
     2521 atagcctacg acagcgacca gccccacgcc caccatcagc tccgtcgtgg cgagcggcac
     2581 cacgccgcgc cccgcggttt ccaatccgaa catcaccccg gaaagcgcca ccccgcacag
     2641 taccagcccg atcatatcga accgcccccg cggcggctcc cgcacgtcct cgacgaacag
     2701 gctgacaagc acgatcccca gcacgcctat cggcacattg atgtcgaaaa tccagcgcca
     2761 gttgaaatag gtaacaatga aaccgcccac cggcggcccc agcaccggcc ccagcagcgc
     2821 cggcatcgtc agccacgcca tcgccgctac cagctcgttc tttggcgccg tccgcagcag
     2881 cacaaggcgc cccaccggca ccatcatcgc cccgccaatg ccttgcagca cgcgcgagcc
     2941 caccaggaac gccaggctgt ccgcccgtcc gcacagaacc gagcccaccg tgaacacggc
     3001 gatcgctgcc cggaacaccg tccgcgttcc gaaccggtct gccacccacc cgctcgccgg
     3061 aatgaacacc gccagcgaca gcaggtacga tgtcagcgcc acgttcatat gcaccggatc
     3121 ggcgccgaac gccttcgcca ttgtgggcag ggcggtcgcg atcaccgtcg agtcgaggtt
     3181 ctgcatgaac aatgcgcagg ccacgatcac ggcagtgagg cgggttcggc cgctggtgcg
     3241 gattctgggg gaggctggac gcaggctcat tgatccccgg tcagtagcat tcaggggacc
     3301 ggctgactat gtgcagctgt gttatgtcag cccagggatt cgcgctgttc gacaccccga
     3361 tcggtccatg cggcatcgtg tggagcgata cggccgtcgt cggcatgcaa ttgccggaac
     3421 gaaacgggcc aaccgcgcgc tcgcggatac aacgcaggca tcccaccgct cgcgaggccc
     3481 cagtccccga ccacatccag cccgtgattg gcgcgatcac cgcactgctg ggcggcgaaa
     3541 agcgtgacct ggcggacatc ccggtcgacc tgaccggcct ctcctcattc cagcggcgcc
     3601 tgtacgagac gctacgcacc gtcccgccgg gcgccaccat ctcctacggc gaacttgcca
     3661 cccgcctggg cgacggcgtc accgcgcgcg acgtgggcga ggccatgggg cagaacccgg
     3721 tcccgatcat cgtcccgtgc caccgggttc tcgcgtccgg cggcaagctg ggcggcttct
     3781 ccgccgccgg cggagtcgcc accaaggtcc ggctgctgga gattgagggt gcaccgattg
     3841 gcgaggcacc caccctcttc ggcgatctgc cgttggccgc gaagccgggg cgacgatagc
     3901 gatagcgcct aatgcgctat cgcctccacc tccaaccgac gggtcgccgg tcccagcgtc
     3961 gcaacggaca gaatgaccac cgccatgcac cctgcgacaa acaggaagac gcccgtcggg
     4021 ccaaag
//
LOCUS       DATGEM010000052         8710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_904391, whole genome shotgun sequence.
ACCESSION   DATGEM010000052 DATGEM010000000
VERSION     DATGEM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904391"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VKQ27_01500"
     CDS             complement(<1..251)
                     /locus_tag="VKQ27_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87635.1"
                     /translation="MSGADLFRPRRRPLLRLVLALGAAAIILCTTGVIIWLRFNVVPP
                     DCSDPRTLALVRQSLLNRFKLPAGVTIDTIRTLAGGYVA"
     gene            complement(254..1612)
                     /locus_tag="VKQ27_01505"
     CDS             complement(254..1612)
                     /locus_tag="VKQ27_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY87636.1"
                     /translation="MSVPFDRVIIGDVVTSDTIIAGGYVAVRGETIAAIGEGVPPPAR
                     EIADHSGKLILPGLVDGHMHTSSSTGWPGIETATKCAAAGGVTTCVDMPYDVPVPVTD
                     AAILADKIGWVVRTAHVDMALYGTITKTGGVGAIPGLAAAGISAFKLSTYEYDAVRFP
                     RIDHPTMLSAFREIAKTGLPVAIHNEDQELVEMLTAQARAAGCAAAIMHCRTRPPLAE
                     TLADLEIFEMALETGAHVHIAHSSLSRGFELAETFRGMGAQTTGEACIQYLCMTEDDI
                     VRLGGKGKCNPPFRTADEVERMWQSLVAGKIAYVSTDHAPWPLERKTLPDIFANSAGL
                     PGLQSFAPLMFSLLDERGLSPTLMATYCAERSARMHGLWPRKGALRVGSDADVCALER
                     GNFIFDEASIQDRPEMRWSPYHGRKMRTRVAATYLRGRLIWDGTTVLTKPGDGRFVRR
                     MG"
     gene            1701..3200
                     /locus_tag="VKQ27_01510"
     CDS             1701..3200
                     /locus_tag="VKQ27_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY87637.1"
                     /translation="MILHDVRVHPSKDNLKREDQLAWKIAAVAADKVAVEKDVAAMIV
                     NRIIDNAAVALGAINRRPVVTARDMALGHPRKDAATVFGVPATKRVSPEWAAWANGTA
                     VRELDMHDTFLAQDYSHPGDNIPPVLAVAQTMGKSGRDLIRGIATGYEIHIDLVRAIS
                     LHKHKIDHITHLCAGQAAGIGTLLNLKPEVIYQAVQQAVHVSVTTRQSRKGEISSWKA
                     YAPAHAGKLAVEAVDRVMRGEGAPSPIYEGEDSVIAWVLDGPQAHYQVPLPAPGEPKR
                     AILDSFTKQHSAEYQSQALIDLAFRMREKIANIEDIAKIIIHTSHHTHAVIGTGANDP
                     QKMDPKASRETLDHSIMYIFAVALQDGKWHHVNSYTPRRAGRADTVRLWHKIETREDP
                     EWERRYLSTDPAEQAFGGRIEIQLQDGSSIVDEMAVANAHPLGAKPFGRDDYIRKFNE
                     MSDGVISTREANRFLEAAQDVARLKAGELHTLNVALPAGTLTAGKPGIF"
     gene            3359..4513
                     /gene="prpC"
                     /locus_tag="VKQ27_01515"
     CDS             3359..4513
                     /gene="prpC"
                     /locus_tag="VKQ27_01515"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HLY87638.1"
                     /translation="MSDTAAIPNRAKRGVGLSGVTAGNTALCTVGRTGNDLHYRGYDI
                     LDVAESCEFEEIAYLLVHGSLPTRAELAGYKAKLRSLRGLPASLKQALEALPPASHPM
                     DVMRTAVSVLGCALPEKEDQNQPGAKDIIDRLMACLGSALLYWFHYAHNGKRIDVETD
                     DDSIGGHFLHLLHGKPPSEMFVRAMHTSLILYAEHEFNASTFTCRVVAGTGADMHSAI
                     TAGIGALRGPKHGGANEVSFDIQSRYDTPEEADADIRKRVANREVIIGFGHAVYTIAD
                     PRNKVIKEVARRLSQQAGSMKLFNIADRIEATMSDAKKMFANLDWFSAISYHMLGVPT
                     SMFTPLFVIARTSGWGAHVIEQRVDNKLIRPTANYIGPENVPFVPIDKRG"
     gene            4874..5206
                     /locus_tag="VKQ27_01520"
     CDS             4874..5206
                     /locus_tag="VKQ27_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87639.1"
                     /translation="MSDEESTTTEEPRAQAMAMRAGGKKKAAKKAAPKKKGAKKAAKK
                     AAPKKKAAAKKKGAKKGAPKKKAAGKKKAAAKKKGGAKKGARKKGGGRKKAAAAPMDA
                     TPSGDSMS"
     gene            complement(5479..6114)
                     /locus_tag="VKQ27_01525"
     CDS             complement(5479..6114)
                     /locus_tag="VKQ27_01525"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HLY87640.1"
                     /translation="MTADDEARHLLVDLAASLFARGFSVGSAGNISVRLPEGYLITPT
                     NSSLGRLDAARISRLDADFKHVDGDRPSKEVFMHRAFYTARQDAGAVVHLHSTMATAV
                     ACLPDVDAANPIPPLTPYFVMRVGRTMPIVEYYRPGDPAMEPAIRTAASDARAVLLAN
                     HGPVVSGKTLTDAVYAAEELEEAAKLYLLLRGTTPRLLTGRQVDDLLTTFG"
     gene            complement(6111..7373)
                     /gene="otnK"
                     /locus_tag="VKQ27_01530"
     CDS             complement(6111..7373)
                     /gene="otnK"
                     /locus_tag="VKQ27_01530"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HLY87641.1"
                     /translation="MLLGAIADDFTGATDLCSMLVRGGMRTVQLIGVPRPGEPAPDAD
                     AVVVALKSRTAPVHQAVEESLTALQWLRGIGCEQFFFKYCSTFDSTDAGNIGPVADAL
                     IAALGGGFALACPAFPANARSVYQGHLFVGSVLLNESGMEHHPLTPMTDPNLVRVLGR
                     QTDGTVGLVPFVTVDQGAAAIREAMAALQGQGRRYAIVDAVSDRHLMEIGEAAGEHKL
                     ITGGSGVAMGLPENFRRAGKLAERDAASLPGVAGAAAVLAGSCSRATLGQLDYVRGRV
                     PVLQLDPLGTPDAARLAAGARDWVADKLGDKPIVIAASAPPEQVAELQRRLGRDGAGA
                     LVEQALASIADELVARGVRRLVVAGGETAGAVVSRLRVHSLRIGGEIDPGVPWTYAEG
                     SGAPLLMALKSGNFGAHDFFLKAFEVLS"
     gene            complement(7378..8193)
                     /locus_tag="VKQ27_01535"
     CDS             complement(7378..8193)
                     /locus_tag="VKQ27_01535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY87642.1"
                     /translation="MIDTFEPDWLALREPFDHAARSVILARRLVQRLPQRPRIVDLGA
                     GTGSMFRYLAPIIGGRQDWLLVDANTGLLDEAFGRTAAWARRRGCTAVATGNELQIAT
                     PQGLWRMEVIAADLGEEFPLAADAVVCSALLDLVSARWLDRLFDRMHAPFLASLIVDG
                     RDIWRPRDPFDAQPRAVFRHDQFRDKGIGRALGATAWAHALRSLAARGFVTASAPSDW
                     HIPRTALGMQRALIDGRGANDARWQEVRLRQALRGRLAITIGHRDILAFPPGG"
     gene            complement(8190..>8710)
                     /locus_tag="VKQ27_01540"
     CDS             complement(8190..>8710)
                     /locus_tag="VKQ27_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY87643.1"
                     /translation="IGTLVPRKGHDVLLRAVARLFDLDWRLIIVGAADHDPVHARLLT
                     ALAEDLGIARQVDFVGEVTGDALEALWQKADLFALATHWEGYGMAIAEALKRGVPVAV
                     SDGGAAGKLVTEMSGVVCPVGDWINLSKALRRMIFGAELRYEMAEAAWQVGQTLPDWQ
                     TQVLEFAEALTG"
BASE COUNT         1777 a         2980 c         2594 g         1359 t
ORIGIN      
        1 aacgcgacgt aaccgccggc cagcgtccgt atggtgtcga tcgtcacacc ggcgggcagc
       61 ttgaaacggt tcagcaggct ttgccgcacc agtgcgagcg tccgcggatc gctgcaatcc
      121 ggcggcacca cgttgaaccg cagccagata atgacaccgg tggtgcacag aatgatcgcg
      181 gcagcaccaa gtgccaggac aaggcgcaac aacggccggc ggcgcgggcg aaaaagatct
      241 gcgccgctca cgcttatccc attcgcctga caaaccttcc atcaccaggc tttgtcagta
      301 cagtcgtgcc atcccagatc agcctgccac gcagatacgt cgcggcgacg cgcgtgcgca
      361 tcttgcgccc atggtacggc gaccagcgca tctcgggccg atcctggatg ctcgcctcgt
      421 caaaaatgaa gttgccgcgc tccagcgcgc aaacatcggc atccgatccg acgcgcaatg
      481 cccccttccg cggccacaac ccatgcatcc gcgcactgcg ttcagcgcaa tacgtggcca
      541 tcagggtcgg tgacaaaccg cgttcgtcca gcaacgagaa catcagtggt gcaaagcttt
      601 gcagcccagg cagacccgcg ctgttggcga agatgtcggg cagggtcttg cgttccagcg
      661 gccatggcgc gtgatcggtg gatacatagg cgatcttgcc ggcaacgagg ctctgccaca
      721 tccgttccac ttcatcggca gtgcggaagg gcggattgca cttccccttc ccacccaacc
      781 gcacgatgtc gtcctcggtc atgcacagat actggatgca ggcctcgccc gtggtctgcg
      841 cgcccatgcc acggaacgtc tcggcgagct cgaacccgcg cgagagtgag gagtgcgcga
      901 tgtgaacgtg cgccccggtc tcgagcgcca tctcgaagat ctcgagatca gccagcgtct
      961 ccgccagagg cggacgcgtg cggcaatgca tgatcgccgc ggcgcatccc gccgcgcgcg
     1021 cctgcgcggt gagcatctcc accagctcct gatcctcatt atggatcgcg accggcagcc
     1081 cggtttttgc aatctcgcgg aatgctgaga gcatcgtcgg atggtcgatg cgcggaaacc
     1141 gtacggcgtc gtactcatag gtcgagagct tgaatgcgga gatccccgca gcggccagtc
     1201 cggggatcgc gccgacgccg ccagtcttcg tgatcgtgcc gtacagcgcc atgtccacgt
     1261 gcgcggtgcg taccacccag ccgatcttgt cggcgaggat cgccgcgtcg gtcacgggca
     1321 ccggcacgtc gtacggcatg tccacacacg tggtaacacc gcccgccgcc gcgcacttcg
     1381 tcgctgtctc gatgcccggc caaccggtcg atgacgaggt gtgcatgtgg ccatcgacca
     1441 gtcccggcag gatgagcttg ccggagtggt cagcgatctc gcgcgccggc ggaggcacgc
     1501 cctcgccgat cgcggcgatc gtctcgccac gaacggcgac atagccgcct gcgatgatcg
     1561 tgtcgctggt gacgacgtca ccgatgatca cacggtcgaa tggcactgac attatatata
     1621 ggtgtctccg gcgctcggtt gcggtgtggt gcgcttcacc ttacgaagga tcgcacacgc
     1681 gaaccagccg gaggaaacgc atgatcctgc atgacgttag ggtgcaccca tcaaaggaca
     1741 acctgaagcg cgaggatcaa ctcgcctgga agatagccgc cgtcgccgcc gacaaggtcg
     1801 cggtcgaaaa ggatgtggcg gcgatgatcg tcaaccggat catcgacaac gccgccgtcg
     1861 ccctgggcgc gatcaaccgt cgcccggtgg tgaccgcgcg tgacatggca ttgggccacc
     1921 cgcgcaagga tgccgccacc gtcttcggtg ttcccgcgac caagcgcgtc agtcccgaat
     1981 gggcggcgtg ggccaacggc accgcggtgc gcgaactcga tatgcacgac acattcctcg
     2041 cacaggacta ttcgcatccc ggcgacaaca ttccgccggt cctggccgtg gcgcagacga
     2101 tgggaaaatc gggccgggac ctgatccgtg gcatcgccac gggatacgag atccacatcg
     2161 acctcgtgcg tgcgatcagc ctgcacaagc acaagatcga ccacatcacc catctctgtg
     2221 ccggacaggc cgccggcatc ggcacactgc tcaacctgaa gcccgaggtc atctaccagg
     2281 cagtgcagca ggccgtgcat gtcagcgtca ccacgcggca atcacgcaaa ggcgagatca
     2341 gctcgtggaa ggcctatgcg ccggcgcatg ccggcaagct tgccgtggaa gcggtggacc
     2401 gagtgatgcg cggcgaaggc gcaccgagcc caatctatga aggcgaggac agcgtgattg
     2461 cctgggtgct cgacggccct caggcgcatt atcaggtgcc actgcccgcg ccgggcgagc
     2521 cgaagcgcgc gatcctcgac agcttcacca agcagcacag cgccgaatac cagtcgcaag
     2581 cactgatcga tctcgcattc cgcatgcggg aaaagatcgc gaacattgag gacatcgcga
     2641 agatcatcat ccacaccagc caccacacgc acgccgtgat cggcaccggc gccaatgatc
     2701 cgcagaagat ggatccaaag gcgtcgcgcg agacgctgga ccactcgatc atgtacatct
     2761 tcgccgtcgc cttgcaggac gggaagtggc accacgtcaa cagctacacg ccgcgccgcg
     2821 ccggccgagc cgataccgtg cggctctggc acaagatcga gacgcgcgaa gatcccgaat
     2881 gggagcgccg ctatctgtcc accgatccgg ccgagcaggc attcggcggc cgcatcgaga
     2941 tccagctgca ggacggcagc agcatcgtcg atgaaatggc ggtggcgaac gcgcatccgc
     3001 tcggcgcgaa gccattcggg cgcgacgact acatccgcaa gttcaatgag atgtcggatg
     3061 gtgtgatctc gacgcgtgag gcaaaccgct tcctcgaagc cgcgcaggac gtggcacggc
     3121 tgaaagcagg cgagttgcac acgttgaacg tcgcgctgcc cgccggcacg cttaccgcgg
     3181 gcaagcccgg catattctga cgcaccccgc ccctgtcatt gcgaggagcg gagcgacgaa
     3241 gcaatctcgg cgcgagcgcg ctctcagccc ggaattgccc ggaagggtca gaccccgccg
     3301 ccgctggcgc tcctcgcaat gacaatatcc cggagacgac atcgcaaagg agaaaaccat
     3361 gagcgacacc gcagcaattc ctaatcgcgc gaagcggggc gtcggcctgt ccggcgtcac
     3421 cgcgggcaac acggcgctgt gcaccgtggg gcgcaccggc aatgacctgc attaccgcgg
     3481 ctacgacatc ctcgacgtcg ccgagtcctg cgaattcgag gagatcgcct accttctggt
     3541 gcacggctcg ctgcccacgc gagcagaact cgccggctac aaagcgaaac tgcgctccct
     3601 gcgcggcctt cctgcctcac tgaagcaggc gctcgaagcg ctgccgcctg cctcgcatcc
     3661 gatggatgtg atgcgcaccg ccgtctcggt gctcggctgc gcgctgccgg aaaaggagga
     3721 tcagaaccag ccaggtgcga aggatatcat cgaccggctg atggcgtgcc tcggctcggc
     3781 gctgctctac tggttccact acgcccacaa cggcaagcgc atcgacgtcg aaaccgatga
     3841 cgactcgatc ggtggccatt tcctgcacct cctgcatggc aagccgccca gcgagatgtt
     3901 cgtccgcgcc atgcacacat cgctgatcct ctacgccgag cacgaattca acgcctccac
     3961 gttcacctgc cgcgttgtcg ctggcacagg tgccgacatg cattccgcca tcactgcagg
     4021 catcggcgcg ctgcgcgggc cgaaacatgg cggtgcaaat gaggtctcct tcgacatcca
     4081 gagccgctac gacacccccg aagaggccga tgccgacatc cgcaagcgag tcgccaatcg
     4141 cgaggtgatc atcggcttcg gccacgcggt ctatacgatc gccgatccgc gcaacaaggt
     4201 gatcaaggag gtcgcgcggc ggctttcgca gcaggcaggc tcgatgaagc tgttcaacat
     4261 cgccgatcga atcgaggcga caatgtccga cgcgaagaag atgttcgcca atctcgattg
     4321 gttcagcgcg atctcctacc acatgcttgg cgtgccgacg tcgatgttca cgccgctgtt
     4381 cgtgatcgca cgcacgtccg gttggggcgc gcacgtcatc gaacagcgcg tcgacaacaa
     4441 gctgatacgc cccacagcaa actacattgg tcctgaaaac gtgccgttcg tgccgatcga
     4501 caaacgcggt taagcgattc gcttgagatg aatgaagcgc cgcgacatcg ctgtcgcggc
     4561 gcttttgtgt tgcatcgatg catcgcattt gcagtgcgag caaacttttt tcatcagatg
     4621 cgagaagaaa cggttgcttt ttattgacag cgatgtgcga gacgcggttt agcggaatct
     4681 gcgtgcgcga atcgattcgc acagcaccct cgctgaagag tcaggtgcac gcggcaacag
     4741 aaggacggtc cattgaccgc agcctcgtca acgtgaagta cagtcgaacg agcaccggct
     4801 tcaagtcggt ccgtcgcgcc ttcagtgacg tgcggcattg gcgcgcaccg agaccagaag
     4861 ggagtttcaa gtagtgagcg acgaagaatc cacgaccacc gaagagccac gcgctcaggc
     4921 gatggcgatg cgggctggcg gcaagaagaa ggccgccaag aaggctgcgc ccaagaagaa
     4981 gggcgcaaag aaggctgcca agaaggccgc gccgaagaaa aaggccgctg ccaagaagaa
     5041 gggtgcaaag aagggcgcac cgaagaagaa ggccgctggc aagaagaagg ctgccgccaa
     5101 gaagaaaggt ggagccaaga agggcgcacg caagaagggc ggaggacgca agaaggctgc
     5161 ggctgccccg atggatgcga ctccgtctgg cgactcgatg tcctgacacg agcccacaat
     5221 accggttacc aaaagggcgc ctcacggcgc cctttctctt agatcaacct tcttcgatca
     5281 cgacccatcg cccgctcgtc acagccgacc gcctcttcgt ccgggccagc cgcccctcgt
     5341 catcgtcacc atcgcccctc ttgtcatggc cggccttgag ccggccatca ctttccgcag
     5401 gcacaatggt gcaactttgg gcccaaccac gacgcagaca ccgcagccgt caggcttggc
     5461 atcaccgtcg cgactgctct acccgaacgt cgtcagcaga tcgtccacct gccgtccggt
     5521 gagcaggcgc ggcgtggttc cccgcaggag cagatagagc ttcgctgcct cttccagctc
     5581 ctcggctgcg taaaccgcgt ccgtgagtgt cttgcccgac accaccggac catgattcgc
     5641 gagcagtaca gcccgtgcat cactggccgc cgtgcgaatt gccggctcca tcgccggatc
     5701 acccgggcga taatactcaa cgatcggcat ggtccgccca acgcgcatca cgaagtacgg
     5761 cgtcagcggc ggaatcggat tcgccgcatc cacatccggc aggcaggcaa ccgcggtcgc
     5821 catggtggaa tgcagatgca ccaccgcgcc ggcgtcctgg cgggcagtat agaacgcgcg
     5881 gtgcatgaac acttccttgg acggccggtc accgtcgaca tgcttgaaat cggcgtccag
     5941 ccgagaaatg cgcgcggcat ccaggcgccc gaggctcgaa ttggtcggcg tgatcagata
     6001 gccttcgggc aggcgcacac tgatgttgcc cgcgcttcca accgagaagc cacgtgcgaa
     6061 caggctcgct gcaagatcga ccagcaggtg gcgagcttca tcatcagccg tcatgacagc
     6121 acctcgaatg ccttcagaaa gaagtcatgc gcgccgaaat tgccggactt cagcgccatc
     6181 agcagcggcg cgccactgcc ttccgcataa gtccaaggca cgccaggatc gatctcgccg
     6241 ccaatccgca ggctatgcac gcgcagccgc gacaccaccg cacccgcagt ttcgccgccg
     6301 gcaaccacca gtcgccttac accacgcgcc accagctcat cagcgatgct ggccagcgcc
     6361 tgctcgacca gcgcgccagc gccatcgcga cccagacggc gctgcaattc agcaacctgc
     6421 tcaggtggcg ccgacgcggc aatcacgatc ggcttgtcac caagcttgtc cgcgacccag
     6481 tcgcgcgcac cagcagccag ccgcgccgcg tccggcgtgc cgagcggatc gagttgcagc
     6541 actggcaccc gcccacggac atagtccagc tggccgagcg tcgcacgcga acacgaacct
     6601 gccagcacag cggccgcacc cgccacccca ggcagcgaag cggcatcgcg ctccgccaac
     6661 ttaccggcgc gacggaaatt ctccggcaag cccatcgcga ccccggatcc gccggtgatc
     6721 agcttatgct ccccagccgc ctcgccgatc tccatcagat gccgatcgct gactgcatcg
     6781 acaatcgcat agcgccgccc ctgcccctgc aacgcagcca tcgcctcgcg gatcgccgct
     6841 gcgccctgat ccactgttac aaacggtacc agcccgaccg tgccatcggt ctgccgccca
     6901 agcacgcgca ccagattggg atccgtcatc ggtgtcagcg gatgatgttc catcccgctc
     6961 tcgttcagca gtacggagcc gacgaaaaga tgcccctggt aaacgctgcg cgcattggct
     7021 ggaaacgccg gacaggcaag cgcgaaaccg ccgccgagcg ccgcgatcaa cgcgtcggcc
     7081 actgggccga tattgcccgc atcggtgctg tcaaacgtgg agcagtactt gaagaagaac
     7141 tgctcgcagc cgatgccgcg cagccattgt aacgcggtga ggctctcctc taccgcctga
     7201 tgcactggtg ccgtgcgtga tttcaacgcg acaaccaccg cgtcagcatc aggcgccggc
     7261 tcacccgggc gaggcacacc gataagctgc accgtcctca tgccgccgcg cacgagcatc
     7321 gagcaaagat cggtagcgcc ggtgaaatca tcagcgatcg cgcctagcag catggcgtta
     7381 acctcccggt gggaaggcaa gtatgtcgcg gtggccgatg gttatggcaa gtctgccgcg
     7441 caacgcctga cgcagtcgca cctcctgcca gcgcgcatca ttcgcgccac ggccatcgat
     7501 caatgcgcgc tgcatgccaa gcgcagtgcg aggaatgtgc cagtcacttg gcgccgatgc
     7561 ggtcacgaac ccgcgcgcgg ccagcgagcg gagggcatgc gcccatgctg tcgcgcctag
     7621 cgcacgacca atgcccttgt cgcgaaactg gtcgtggcgg aacaccgcgc gtggctgagc
     7681 atcgaacgga tcccgaggcc gccagatgtc gcggccgtca acgatgaggc tcgccagaaa
     7741 cggggcatgc atccggtcga acagacgatc gagccaccgc gcggaaacga gatcgagcag
     7801 ggcgctgcaa acgacagcat ccgcagcaag ggggaactcc tcccccagat ccgccgctat
     7861 cacttccatc cgccacaacc cttgtggcgt ggcaatctgc agctcattgc cggtcgctac
     7921 agccgtgcac cctcgccgcc ttgcccatgc cgcagtgcga ccgaatgcct cgtcgagcag
     7981 ccccgtattg gcatccacca gcagccaatc ctgccgccca ccgatgatcg gcgcgagata
     8041 gcggaacatg ctccccgtgc cggcaccaag gtccacgatc cgtggacgct gcggcagtcg
     8101 ctgcaccagt cgccgcgcca ggatcacgct gcgcgcggca tgatcgaacg gttcgcgcag
     8161 agcgagccag tctggctcga aagtgtcgat caccccgtca gggcctcggc gaattccagc
     8221 acctgcgtct gccaatcggg caatgtctgc cccacctgcc acgcggcttc cgccatctcg
     8281 tagcgcagct ccgcaccaaa aatcatgcgg cgaagtgcct tggagagatt gatccagtcg
     8341 ccgaccggac agaccacgcc gctcatctcg gtcaccagct tccccgccgc accgccatcc
     8401 gagaccgcaa ccggcacgcc acgcttcagc gcctcggcaa tagccatgcc gtagccttcc
     8461 caatgtgtcg ccagcgcgaa caggtcggcc ttctgccata gcgcttcgag cgcatcgccc
     8521 gtcacctcgc cgacgaagtc cacctgccgc gcaatgccca gatcctcggc caatgcggtc
     8581 agcagccgcg catgcaccgg atcatgatcc gcggccccca cgatgatcag tcgccagtcc
     8641 agatcgaaca gccgtgccac cgcgcgcagc agcacatcat gtcccttgcg cggcaccagc
     8701 gtgccgatgg
//
LOCUS       DATGEM010000053         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1695304, whole genome shotgun sequence.
ACCESSION   DATGEM010000053 DATGEM010000000
VERSION     DATGEM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695304"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VKQ27_01545"
     CDS             <1..1216
                     /locus_tag="VKQ27_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HLY87644.1"
                     /translation="ETAKQAALGETVFGVRTVKSLALEPQRKQLWDERIAEAGKWRLA
                     YGKLGNWPQTMVTPIERFMWMGVILIGVYWALTDTTGYGVGSLFAFMMLSQRVAQPLV
                     QLAKLTDEYQDVAASIGQAADVLNRPLEVDAASGGMRPRFAGAITFEDVTFTYPGTKT
                     PALDRISFGVPAGTMLGIVGRSGSGKSTITRLLQGINRDYAGFLKIDGADLREINLRH
                     LRTSFGVVLQDNFLFRGSIRDNILAGRPGLTLEDAVRAARLAGAEEFIERMPNGYETY
                     IEEGSPNLSGGQKQRLAIARALICDPRILILDEATSALDPESEALVNANLLRIARGRT
                     MVIVSHRLSSLTECDQILVLEKGKVMDIAPHRVLVERCAVYRQLWAQQNRHLDSQGPR
                     HAALAPTLVQGD"
     gene            1179..2588
                     /locus_tag="VKQ27_01550"
     CDS             1179..2588
                     /locus_tag="VKQ27_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HLY87645.1"
                     /translation="MPLSPQLSSKAISPAVEDRNDPTLPVILEFQMPSTAITTTPVPR
                     SARRIVWILGSMLALMMLALGLIKVDRVVTAQARIVSKNPTLVLQPLEASIVRSIDVH
                     EGEDVHAGQVLARLDPTFAAADEGALAAQVASLQAQVSRLQAEANGKQFAYTGIDPDL
                     SLQAAIFAQRQSEYTYKIENYTQKIHGLVAAIGKAKADETGYRDRLQVASQVETMRRD
                     LERMQVGSKLNTLAAQDNRAEMQRNLQGAIDQENSAERDLSAMVAERDGYVQSWHADL
                     SEKLADATSKLSDARESLNKAQLRRQLVVLRAEKDGTVLTVAKVSVGSVLQSGQQFIT
                     LVPSDAPLEVEANISGRDDGFVHVGDEVALKLDTFPFMQYGLAYGAVRTISADSFTAQ
                     DDQRNPTGAVPMPGNTSDPYYRARIAIDRVELHGTPKGFRIMPGMPVTADIKVGKRTV
                     LSYLLGRVLPVASEGMREP"
     gene            2616..>3584
                     /locus_tag="VKQ27_01555"
     CDS             2616..>3584
                     /locus_tag="VKQ27_01555"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HLY87646.1"
                     /translation="MALFDQLLARTSPAAALRRGLKLTEQGDLKRAFPLLTRAARAGI
                     AEAEFRVGRCYLEGAGVPPSRTEGARWLERAANQGYVEAQTQLATLSIHGLLNGMGSD
                     ASGGAKLFASNAPAEPDFETGIKWARKAAEAGSADGQAVLGFILTSGPENLRDLEAAD
                     QWYERSAAAGCPPGALGYALALARNATNPEQHTKVAELLNRAAEAGLPTALYLLAIMV
                     ERGLGVPADKAEAIQLFRKAAERGNRPSQARWGLALMEGITVPANPVDGESWLRRAAL
                     AGDPEAAAVVGDLYARGGKLPPNYAEAAIWFRHAADAGHRGAARALG"
BASE COUNT          639 a         1092 c         1257 g          596 t
ORIGIN      
        1 ggagacggcg aagcaggcgg cactgggcga gactgtgttc ggtgtgcgca cggtgaagtc
       61 gctggcgctg gagccgcagc gcaagcaact gtgggacgaa cgcattgccg aggccggcaa
      121 gtggcgcctt gcctatggca agctcggcaa ctggccgcag acgatggtga cgccgatcga
      181 gcgcttcatg tggatgggcg tgattctcat cggtgtctac tgggctctga ctgatacgac
      241 cggatacggg gtcgggtcgc tgtttgcctt catgatgcta tcgcagcgtg tcgcccagcc
      301 gctggtgcag cttgccaagc tgaccgacga gtaccaggac gttgcggcct cgatcggcca
      361 ggcggccgat gtgttgaacc ggcctctgga ggtggacgcg gcgtcgggcg gcatgcggcc
      421 gcgcttcgca ggcgcgataa ccttcgagga cgtgacgttc acctatccag gcaccaagac
      481 accggcgctg gaccggatca gcttcggcgt gccggcggga accatgctcg gcattgtcgg
      541 gcggtccgga tccggtaaat cgacgatcac ccggctgctg caaggcatta accgggacta
      601 cgcgggtttc ctgaagatcg atggcgcgga tctgcgcgag atcaacctcc gccatctgcg
      661 caccagcttc ggtgtcgtgc tgcaggacaa tttcctgttc cgcggctcga tccgcgacaa
      721 catcttggcc gggcgtccgg ggctcactct ggaagatgcg gtgcgcgccg cgcggctggc
      781 gggcgcggaa gagttcatcg agcgtatgcc gaatggctac gaaacctaca tcgaggaagg
      841 ctcgccaaat ctttcgggag ggcagaagca gcggctcgcg attgcgcgcg cgctgatctg
      901 cgatccgcgc atcctgatcc ttgatgaggc aaccagtgcg ctcgatccgg agagcgaagc
      961 gctggtgaat gccaatctgc tgcgcatcgc gcgcggccgg acgatggtca tcgtttcgca
     1021 ccggctgtcg tcgctgacag agtgcgacca gatcctcgtg ctggagaagg gcaaggtgat
     1081 ggacatcgcg ccgcatcgag tcctggtcga acgctgcgcg gtctatcgcc agctttgggc
     1141 acagcagaat cgtcacctcg acagccaagg tccacgccat gccgctctcg ccccaactct
     1201 cgtccaaggc gattagcccc gccgtcgagg accgcaacga ccccacgttg ccggtcatcc
     1261 tggagttcca gatgccatcg acggcgatca cgacgacgcc ggtgccgcgt tcggcgcggc
     1321 ggattgtctg gatcctcggc agcatgctcg cgctgatgat gctggcgctg gggctgatca
     1381 aggtcgatcg cgtggtgacc gcgcaggcgc gcatcgtctc gaagaacccg accctcgtgc
     1441 tgcagccgct ggaagcctcc atcgtgcgct cgatcgacgt gcacgagggc gaggacgtgc
     1501 atgccgggca ggtcctggcg cggctcgatc cgacatttgc cgcggccgat gagggcgcgc
     1561 ttgcagcaca ggtggcaagc ctgcaggcgc aggtgtcccg gctgcaggcc gaagccaacg
     1621 gcaagcagtt tgcctatacc ggcatcgatc cggacctgtc attgcaggcg gcgatcttcg
     1681 cacagcgcca gtccgagtac acttacaaga tcgagaacta cacgcagaag atccatggcc
     1741 tggtggccgc cattggcaag gcgaaggcgg atgagaccgg ctatcgcgac cgccttcagg
     1801 tagcttcgca ggtggagacc atgcggcgtg acctggagcg gatgcaggtc ggcagcaagc
     1861 tcaacacgct cgccgcccag gacaaccgcg ccgagatgca gcgcaacctc cagggcgcaa
     1921 tcgaccagga gaacagtgcg gaacgcgatc tctcggcgat ggtcgcggaa cgtgatggct
     1981 atgttcaatc ctggcatgcc gatctgtcgg agaaactcgc cgacgcgacc agcaagctgt
     2041 cggatgcgcg cgagtcactg aacaaggcgc agcttcgccg tcagcttgtg gtgcttcggg
     2101 cggaaaagga cggaaccgtg ctgacggtcg ccaaggtctc ggtgggttcg gtgcttcagt
     2161 ctggccagca gttcatcacg ctggtgccaa gtgatgcgcc actggaggtg gaggccaaca
     2221 tctccggtcg cgacgacggg ttcgtgcatg ttggcgacga ggtggcgctc aagttggaca
     2281 cgttcccgtt catgcaatac ggcctggcgt atggggcagt ccgcaccatc agcgcggaca
     2341 gcttcactgc gcaggatgat caacggaatc caaccggcgc tgtgccgatg cccggcaata
     2401 cgagcgatcc ctactaccgc gcacgcattg cgatcgatcg ggtcgaactg cacggaaccc
     2461 cgaaaggctt ccgcatcatg cctgggatgc cggtgacggc ggacataaag gtgggcaagc
     2521 gcaccgtgct cagctacctg ctcgggcgtg tgctgccggt ggcctcggag ggcatgcgcg
     2581 agccgtgact acccgggcaa gggaagcctg acgcaatggc cttgttcgac cagctgcttg
     2641 ccaggacgtc gcctgcagcg gcgttgcggc gtgggctgaa gctgacggag cagggagacc
     2701 tgaagcgggc gttcccgctg ctgacgcgcg cggcgcgtgc ggggatcgct gaggcggagt
     2761 tccgggtcgg tcggtgctat ctggaaggtg caggcgtgcc gccaagccgc acagagggcg
     2821 cacgctggct cgagcgagcg gcgaaccaag ggtatgtcga ggcgcagacg caactggcga
     2881 cgctgtcgat ccacggattg ctgaacggca tgggatcgga cgcaagcggg ggagcgaagc
     2941 tgtttgccag caatgccccg gcggagcctg attttgagac cggcatcaaa tgggcccgga
     3001 aagcggcaga ggcgggatcc gccgatggcc aggccgtgct gggcttcatc ctcacctccg
     3061 ggccggagaa tctacgcgac ctcgaagcag ccgatcagtg gtacgagagg tcagcggcgg
     3121 ctggctgtcc gccgggtgcg ctcggctacg cgttggcgct ggcccgaaat gcgaccaacc
     3181 cggagcagca tacgaaggtc gccgaactgc tcaaccgcgc tgccgaagcc ggtctgccga
     3241 cggcacttta tctgctggcg atcatggtcg agcgtgggct cggcgttcct gctgacaagg
     3301 cagaggcgat ccagctgttc cgcaaggcgg cggagagggg caaccggccg agccaggccc
     3361 gctggggcct ggcgctgatg gagggcatca ccgtgcccgc caacccggtc gatggcgaat
     3421 cctggctgcg gcgggctgcg ttggcgggag atcccgaggc cgcggccgtc gtcggcgatt
     3481 tgtatgcgcg aggcggcaag ttgccgccga actacgctga ggcggcgatc tggttccggc
     3541 atgccgccga tgccgggcat cgcggggccg cacgtgccct gggg
//
LOCUS       DATGEM010000054         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_97193, whole genome shotgun sequence.
ACCESSION   DATGEM010000054 DATGEM010000000
VERSION     DATGEM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97193"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VKQ27_01560"
     CDS             <1..739
                     /locus_tag="VKQ27_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY87647.1"
                     /translation="APFVAARGAKVWLEVPRLLRRLAATVEGVAGTIVPGEPLPDCDF
                     QCPLLKLPMAFGTDAGNIPASVPYIQADRQAVAAWRQRLGATRQRRVGIVLSGNNDHR
                     ENRLRSIPVARLLPLLRRQDCEFHVVQTGLAKDDAAQLAELGVQDHSGVLSDFADTAA
                     LMSLLDLIISVDSAPAHLAGALARPTWLLPQFSADWRWQCDRTDSPWYPTMRLFRQPR
                     PGDWDSVVTEVGRALDTMPAVTPAPPR"
     gene            complement(717..1193)
                     /locus_tag="VKQ27_01565"
     CDS             complement(717..1193)
                     /locus_tag="VKQ27_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLY87648.1"
                     /translation="MADRLAWEFAFPSIGEGKLALDAFKGRVLLVTNTASFCGYTNQY
                     AELEKLHETMEVRGLTVLGVPSRDFNQESADNATVKAFCETRFGVQFPMAGLCHVRGA
                     EAAPFYAWVREETRWEPGWNFCKVLVGRDGRVVGTFGSGDEPQGAKLSAAIGAALG"
     gene            complement(1186..1824)
                     /locus_tag="VKQ27_01570"
     CDS             complement(1186..1824)
                     /locus_tag="VKQ27_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HLY87649.1"
                     /translation="MSNDKLIEAKRKWAREGRLLTGTVGDPERDRLPPGQRLTRDWPV
                     LDLGAEPDVTPQKFRLDVDGAVENRLSLSLDEFMALPMTESVSDIHCVTAWSRYDNHW
                     KGVAAQDLLRIVEPKPEAQHIVLHSFDGYSTNIRLDQFDQPDVFLVHEWEGKPLSRAH
                     GGPVRMLLPRLYFWKSAKWLRRIQFTISDHPGFWERRGYHNNGDPWTEERYG"
     gene            complement(1825..2196)
                     /locus_tag="VKQ27_01575"
     CDS             complement(1825..2196)
                     /locus_tag="VKQ27_01575"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HLY87650.1"
                     /translation="MAALVLAGPAWAHGLECPAHAPADWHASPGALNGVQVLSAKQGE
                     KIDDQSPPTLVPDDQATRNGVLHSVWQMNGEGPEWQFFVWCHYAGSERVLKLAASQVK
                     RCEYTTPAAHPDRPPQAMVCD"
     gene            complement(2214..2699)
                     /gene="smpB"
                     /locus_tag="VKQ27_01580"
     CDS             complement(2214..2699)
                     /gene="smpB"
                     /locus_tag="VKQ27_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HLY87651.1"
                     /translation="MAEAKTKKGLISHGIAAQNRKARFDYSIKDTVEAGLVLKGPEVK
                     SLRHGRATLSEAWAGERDGELWLFNAYIPEYQGGVLSRFEPRAPRKLLLKRKQIRSLA
                     GAVAREGVSLVPLQIHFNERGRAKVLLGLGEGRKKQDKRHAIADRDWQRDKARLMRAR
                     G"
     gene            complement(2745..>3412)
                     /gene="dapA"
                     /locus_tag="VKQ27_01585"
     CDS             complement(2745..>3412)
                     /gene="dapA"
                     /locus_tag="VKQ27_01585"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLY87652.1"
                     /translation="PVIAGAGSNSTKEAIELAEHAEKAGANAVLVVTPYYNKPTQEGM
                     YLHFKAIADAVDIPMIIYNIPPRSVVDMSVDTMARLAKHRNIIGVKDATANLTRPLHT
                     RRACGEDFCQLSGEDHTTLSFNAAGGVGCISVTANVAPRLCAAMQDAWAEGRFSEAMA
                     IQNRLVPLHDALFTETSPAPVKFAASLLGKTSEHCRLPLAPLMESTRTKVRAAMTEVG
                     LLN"
BASE COUNT          536 a         1203 c         1035 g          638 t
ORIGIN      
        1 tgcgccattc gtcgccgcgc gcggtgcaaa agtctggctc gaggtacccc ggctgctgcg
       61 ccgtctggca gcgacggtgg aaggcgttgc cggcaccatc gtccccggtg agccgttgcc
      121 tgactgcgac tttcagtgcc cgttgctcaa gctgccgatg gcgttcggca ccgacgcggg
      181 caacattccc gcttcagttc cgtatataca agccgatcga caggcggttg ccgcttggcg
      241 gcagcgtctt ggtgcgacgc gacagcgaag ggttggcatc gtcctgtccg gtaacaacga
      301 ccatcgggag aaccggctgc gttcgatccc ggtggccagg ctgctgcctt tgctgcgccg
      361 tcaggactgc gaattccacg tcgtccagac cgggctcgcc aaggacgatg cggcgcagct
      421 tgcggaactc ggcgtgcaag accacagcgg agtgctgtcc gactttgccg ataccgccgc
      481 cctgatgagc ctgctcgacc tcatcatctc ggtggattcg gcccctgccc atctggccgg
      541 cgcgctcgcc cgtccgacct ggctgctgcc tcagttcagc gccgattggc gctggcagtg
      601 cgaccgcacc gacagtccgt ggtatccgac gatgcgcctg ttccgtcagc cgcgcccggg
      661 cgattgggac agcgtcgtga ccgaagtggg cagggcgctg gacaccatgc cggccgttac
      721 cccagcgccg ccccgatagc cgccgatagc ttcgccccct gcggctcatc gcctgatccg
      781 aacgtgccca ccacacgccc gtcgcgtccg accagcacct tgcagaaatt ccagcctggc
      841 tcccaacgcg tctcttcacg cacccaagca tagaacggcg ccgcctctgc accgcgcacg
      901 tggcacaggc cggccatcgg gaattgcacg ccgaaccgcg tctcgcagaa tgccttcacc
      961 gtggcattat ccgccgactc ctggttgaag tcgcggctcg gcacgccgag caccgtcagc
     1021 ccgcgcactt ccatcgtctc gtggagcttc tccagttctg catattggtt tgtatagcca
     1081 cagaagctcg ctgtgttggt cacgagcaag actcgcccct tgaatgcatc cagtgccagc
     1141 tttccctctc cgatcgaggg aaacgcgaac tcccacgcca gccggtcagc catacctttc
     1201 ctccgtccag gggtcgccgt tgttatggta gccgcgccgt tcccagaaac cgggatggtc
     1261 gctgatcgtg aactggatcc gtcgcagcca cttggccgac ttccagaaat acagccgggg
     1321 aagcagcatc cgcaccgggc cgccatgcgc acggctcagc ggcttgccct cccattcatg
     1381 caccaggaac acatccggct gatcgaactg atcgagccgg atattggtgg aatatccgtc
     1441 gaaactgtgg agcacgatgt gctgcgcctc tggcttcggc tcaacgatgc gcagcaggtc
     1501 ctgcgccgcc acgcccttcc agtggttgtc gtagcgcgac caagcggtga cgcagtggat
     1561 gtccgacacg ctctcggtca tcggcagcgc catgaactcg tcgagggaca ggctcagccg
     1621 gttctcgact gccccatcga cgtccagccg gaacttctgc ggtgtgacat ccggttccgc
     1681 gcccaggtcc agcaccggcc agtcgcgggt cagccgctgg cctggcggca gccggtcacg
     1741 ctcgggatcg ccgacggtgc cggtgagcag gcgtccctcg cgcgcccatt tccgcttcgc
     1801 ctcgatcagc ttgtcgttgc tcattcagtc gcacaccatc gcctgcggcg ggcggtcagg
     1861 atgcgctgcc ggggtggtgt attcgcatcg cttcacctgt gacgcggcca gcttcaggac
     1921 gcgttcgctg cccgcataat gacaccacac gaagaactgc cattctggtc cttcaccatt
     1981 catctgccac accgagtgaa gcacgccgtt tcgcgtggcc tgatcgtccg gcacaagtgt
     2041 cgggggagat tggtcgtcga tcttctcgcc ctgttttgcc gacagcacct ggacgccgtt
     2101 cagtgcgcca ggggaggcat gccaatccgc tggcgcatgg gccgggcatt ccaaaccgtg
     2161 cgcccatgcc ggcccagcca gcaccagcgc cgccaacgcc aatcggagca tcgtcaccct
     2221 cgtgcccgca tcaatcgcgc cttgtcacgc tgccagtccc gatcggcgat tgcgtgccgc
     2281 ttgtcctgct tcttcctgcc ctcgccgagg ccgagcagca ccttcgcccg cccgcgctca
     2341 ttgaagtgga tctgcagcgg cacgaggctc acgccctcgc gcgccaccgc cccggccagg
     2401 gaccggatct gcttgcgctt cagcagcagc ttgcgcggcg cgcgcggctc gaaccgcgac
     2461 agcacgccgc cctggtattc cgggatatag gcgttgaaca gccagagctc accgtcacgc
     2521 tcgcccgccc atgcctcgga cagcgtcgcg cgcccgtggc gcagcgactt cacctccggc
     2581 cctttcagca cgaggccggc ctccaccgtg tccttgatcg agtagtcgaa ccgcgccttg
     2641 cggttctgtg ccgcgatccc gtgggagatc aggcccttct tggtcttcgc ttcagccatt
     2701 cccctaatat aaccacccgc ttccctgctg ggagggggta ggggtcagtt cagcagcccg
     2761 acctcggtca tggccgcgcg aaccttggtt cgcgtcgact ccatgagcgg tgccagaggc
     2821 agccggcaat gctccgaggt ctttccaagc aacgaagctg cgaactttac cggtgccggg
     2881 ctggtctcgg tgaacagcgc atcgtgcagt ggcacgaggc ggttctggat cgccatcgcc
     2941 tcgctgaacc ggccctcggc ccatgcgtcc tgcatcgccg cgcagaggcg cggcgcgacg
     3001 ttggcggtga cgctgatgca gcccacgccg ccggcggcat tgaacgatag cgtcgtgtgg
     3061 tcctcgccgg agagctggca gaaatcctcg ccgcaggcac gccgcgtgtg cagcggacgc
     3121 gtcaggttcg ccgtcgcgtc cttcacgccg atgatgttcc tgtgcttcgc cagccgggcc
     3181 atcgtatcga cgctcatatc caccaccgag cgcggcggga tgttgtagat gatcataggg
     3241 atgtcgaccg cgtcggcgat cgccttgaag tgcagataca tgccttcctg ggtcggcttg
     3301 ttgtagtacg gcgtcaccac cagcaccgca ttggcgccgg ccttctccgc gtgctcggcg
     3361 agctcgatcg cctccttggt cgaattcgag ccggcgccgg cgatcacggg ca
//
LOCUS       DATGEM010000055         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_664294, whole genome shotgun sequence.
ACCESSION   DATGEM010000055 DATGEM010000000
VERSION     DATGEM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664294"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VKQ27_01590"
     CDS             complement(<1..872)
                     /locus_tag="VKQ27_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87653.1"
                     /translation="MDSSSFRPLASRRSLLLAGAGLASGALLPVSTHEALAQDKPPLG
                     TWPAGSSGSTVFVGLSIPRTGTYAVPGEDELKGAELGIEHINSGHPLMKKIATGLTKG
                     LLGKQIVHGVADSEAKPNTAVQNCTNFINNNKAVLIAGCVSSAEAVALNKLAQREHVI
                     YLPIVSGSNDTTGKDCVRYSFRQCFYAQTAAAALTPVIIKQLGKNKKIAFLTPDYTYG
                     HTVRQSFEEATKEQGSWSVATNQVCPLGASDYSSYLLNIANSGADVFININFGNDAIQ
                     SIKQAKQFGVLDKMT"
     gene            1026..1901
                     /locus_tag="VKQ27_01595"
     CDS             1026..1901
                     /locus_tag="VKQ27_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY87654.1"
                     /translation="MPSLPLLLSQAFNGLALGSLLALVSSGLTIILGTLGVLNFAHGA
                     MFMLGTYAMLVVVQATGSWFLAAGVSTLFMLLVGVVVERVVVRLFYRRPPEDQILVTF
                     GVGIVLVEAVRAVFGSETQHLSTPSWGEGVTRLGFLIYPTYRLELIGIIAIALVALYL
                     ILYRTAIGLVVRAGIEDARMVGILGINVRRTFLLVFAIGVAAAGFAGMLYGPLVSVTP
                     DLGDSFLIQAFVVIVIGGLGSFPGAVVGGLIAGEIISITSAFNSAYSEVMLYAVMAIL
                     LVARPQGLFGTEGRV"
     gene            1898..3703
                     /locus_tag="VKQ27_01600"
     CDS             1898..3703
                     /locus_tag="VKQ27_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLY87655.1"
                     /translation="MRSVVPELVLAAALLATPFVFIHLGASYDLMGRVLDWGILGIGF
                     DLLFGWTGLLSFGQAAFFGTGGFVSAYLLVYHVIGSVWLALAVGTIAAGVFGLIVGWL
                     AVRRIGIYFAMITLAFGQMSFFLENSPLSAYTGGENGIPGVPVPRIGFGSTAYAITTG
                     LPMYWMLAAMFLACFILARRITQSPFGVILRAIKENTARAAMLGHAVPRYKLATFVIA
                     ALFAGLAGGLLGVLQSFMPPDAFALDTSGQLVVQTVIGGVGTLIGPTVGAAVWLWLRD
                     NLQLIPEIGALWKLVLGIVFVVLVIVLRRGICGEIREIWRRRAHASAPPPAPVIAAEV
                     LPLAAPLVRARQDAPALETHGLAKHYGGLRAVDGVSFAIRSNSIHAVIGPNGAGKSTL
                     FKMLLDEVRPTEGDVLLHGSRITGLGATRTAQLGIGKSNQLNHLFLDLTVRENLRIAA
                     LARRRGPFRLDVLRPADSIAEVERQIDTVLATMDLGERANVPAHILAYGEKRRLEIGM
                     ALATGPHVLLLDEPLAGMSPAERTTTRALIRDIGRDRTLVIVEHDMDAVFELAERITV
                     LYEGSLLAEGSPEEIQRNQAVQDAYLGGLREHEPA"
     gene            3690..4388
                     /locus_tag="VKQ27_01605"
     CDS             3690..4388
                     /locus_tag="VKQ27_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87656.1"
                     /translation="MSLLELEAVNTFYGDSHILFDVSMRVEQNEVVALLGRNGAGKTT
                     TLRTIMGVLAPGSGTIRLDGKPIGGLPPYVIARMGMQLVPEERGVFGGLTVEENLRIA
                     ALTAPAPWGLDRIYAMFPRLAERRRSRGGSLSGGEQQMLTIARALIRDARIILLDEPF
                     EGLAPLIVRDLVRISRELAAQGRTIVVVEQNVVAALSFADRVYVLNNGHVVYEGTPAS
                     LHADPEVMKSHLGV"
     gene            complement(4396..>4608)
                     /locus_tag="VKQ27_01610"
     CDS             complement(4396..>4608)
                     /locus_tag="VKQ27_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87657.1"
                     /translation="TLVGTTEKRHCIMGLLLLILIIVLLFGGGGYYGYRSSYYGGAHY
                     GGGFGLILLLLVLFLLFGAPGYWGHY"
BASE COUNT          731 a         1469 c         1480 g          928 t
ORIGIN      
        1 gtcatcttgt ccagcacgcc gaattgcttg gcctgcttga tcgactggat cgcgtcgttg
       61 ccgaagttga tgttgatgaa cacgtcggcg ccgctgttgg cgatgttcag caggtaggag
      121 ctgtagtccg atgcgccgag cgggcacact tggttggtcg cgaccgacca actgccttgc
      181 tccttggtgg cttcctcgaa ggactgccgc actgtatggc catacgtata gtccggcgtc
      241 aggaaggcga tcttcttgtt cttgccgagc tgcttgatga tcaccggcgt cagtgcagct
      301 gccgcggtct gcgcgtagaa gcactggcgg aacgagtagc gcacgcaatc cttgccggtc
      361 gtatcgttag atccactgac gataggcaga taaatgacat gctcgcgctg ggccagcttg
      421 ttcagcgcca ctgcctcggc ggacgagacg cagccggcga tcagcacggc cttgttattg
      481 ttgatgaagt tggtgcagtt ctgtactgcg gtgttgggct tcgcctccga atcggcgaca
      541 ccatggacga tctgcttgcc gagcagcccc ttggtcaggc cggtcgcgat cttcttcatc
      601 agcggatggc cgctgttgat gtgttcgatg ccaagttcgg cacccttcag ctcatcctcg
      661 cccggcacgg cataagtccc ggtgcgcggt atcgaaagcc ccacgaacac cgtcgaaccg
      721 gaggaccctg ccggccatgt gccaagcggc ggcttgtcct gcgccagcgc ctcgtgcgtt
      781 gagaccggca gcagcgcgcc actcgccaac ccggcgcccg ccagaagcag cgaccgccgc
      841 gatgcgagcg gccggaacga tgaactgtcc atggaaacct cccaacttcg gccgtcgcgt
      901 tcctgcggcg gaccattgac cttagccaat tacaggcggc ttgccggccc gcccttgcgg
      961 caacctacta ggcgagagct ttcgcctgtg caagtaaagc accgcccaca acatgacatt
     1021 atcccatgcc ttccctgcca ctcttattgt ctcaggcgtt caatggtctt gccctcggct
     1081 cattgctggc cctcgtcagc agcgggttga caataatttt gggcacgctc ggcgtgctga
     1141 acttcgcgca tggcgcgatg ttcatgcttg gtacctatgc catgctcgtc gtcgtgcagg
     1201 cgaccggatc ctggttcctg gcggctggcg tcagcacgct gttcatgctt ctggtcggtg
     1261 tcgtcgtcga gcgtgtcgtg gtgcgcctgt tctatcgccg tccgcccgag gatcagatcc
     1321 ttgtgacttt cggtgtgggg atcgtgctgg tggaagccgt tcgtgctgtg ttcggcagtg
     1381 agacccagca cctgtccaca ccatcctggg gtgagggcgt tacaaggctc ggcttcctga
     1441 tctacccgac atatcggctg gaactcatcg gcatcatcgc gattgcgctg gtcgcgctgt
     1501 atctcattct gtatcgcacc gcgatcgggc tggtcgtccg tgccggcatc gaggatgccc
     1561 gcatggtcgg catcctcggc atcaacgtgc ggcgcacctt cctgctggtc ttcgccatcg
     1621 gcgtcgccgc ggctggattt gccggcatgc tgtacggtcc attggtctcg gtgacacctg
     1681 atctcggcga ttcattcctg atccaggcgt tcgtggtcat cgtgataggt ggtcttggat
     1741 cgtttcccgg cgccgtcgtc ggcggcctga tcgccggcga gatcatcagc atcaccagcg
     1801 ccttcaactc cgcctactcg gaagtgatgc tctacgcggt aatggcgatc ctcctcgtgg
     1861 cacgcccgca gggtctgttc ggcaccgagg gccgagtatg aggtcagtcg ttccggagtt
     1921 ggtgctcgcc gccgcgctgc tggcgacgcc cttcgtcttc atccacctcg gcgcctccta
     1981 cgatctgatg ggccgggtgc tcgattgggg catcctgggt atcggcttcg acctgctgtt
     2041 cggctggacc ggcctgctgt ccttcggaca ggcggcgttc tttggcaccg gcgggttcgt
     2101 gtcggcctac ctgctggtct atcacgtcat cggcagcgtg tggctcgcgc tcgccgtggg
     2161 caccatcgcg gcaggcgtgt tcggcctgat cgtgggctgg ctcgcggtgc ggcgtatcgg
     2221 catctacttc gccatgatca cgctggcgtt cggccagatg tcgttcttct tggaaaactc
     2281 gccgctgtcc gcctacaccg gcggcgagaa tggtatccct ggcgtcccgg tccccaggat
     2341 cgggttcggc tcaacggcgt acgccatcac aaccgggctg ccgatgtact ggatgctggc
     2401 ggcgatgttc ctcgcctgct tcatcctggc acggcgcatc acccagtcgc ccttcggcgt
     2461 gatccttcgc gcgatcaagg agaacaccgc gcgggccgcg atgctcggcc atgcggtacc
     2521 tcgctacaag ctcgcgacct ttgtgattgc cgcgctgttc gcgggcctcg cgggcggatt
     2581 gttgggcgtg ctgcagagct tcatgccacc ggacgcattc gcattggaca cgtcgggcca
     2641 gctcgtcgtg cagacggtca ttggtggtgt tggaacgctt atcggcccaa cggtgggcgc
     2701 cgccgtgtgg ctctggctgc gcgacaacct gcaattgatc cctgagatcg gggcgctgtg
     2761 gaagctggtg ctcggcatcg tcttcgttgt gctggtgatc gtcctgcgcc gcggaatctg
     2821 tggcgagatc cgcgagatct ggcggcgacg cgcgcatgcc tcggcaccgc cgcccgcgcc
     2881 ggtcatcgcg gccgaggttt tgccgctggc cgcaccgctg gttcgcgcgc gacaggacgc
     2941 accggcactc gaaacgcacg gccttgccaa gcactacggc gggttgcgcg cggtcgatgg
     3001 cgtgtcgttc gcgatccgaa gcaattcgat ccacgccgtc atcgggccca atggcgccgg
     3061 caaatccacg ttgttcaaga tgctgctgga tgaggtgcgg ccgacggaag gcgatgtgct
     3121 gctgcacggc agccgcatca ccggcctggg agcgacgcgg accgcgcagc tcggcatcgg
     3181 caagagcaac cagctcaacc acctgttcct cgacctgacc gtccgcgaga acctgcgtat
     3241 cgctgcgctg gcgcggcggc ggggcccgtt ccgcctggac gtcctgcgcc ctgctgactc
     3301 gatcgccgaa gtcgaacggc agatcgacac cgtgctggcg accatggatc tcggcgagcg
     3361 cgcaaatgtg ccagcgcata tactggcgta tggcgaaaag cgccgactgg agatcggcat
     3421 ggcgttagcg accggcccgc atgtcctgct gctggatgag ccgctggccg gcatgagccc
     3481 cgctgaacga acaactacgc gcgcgttgat ccgcgacatc ggccgcgacc gcaccctcgt
     3541 gatcgtcgaa cacgacatgg acgcggtatt cgaactcgcg gagcgcatca ccgtgctcta
     3601 cgagggctcc ctgctggccg aaggctcgcc ggaggaaatc cagcgtaatc aggcggtgca
     3661 ggacgcttat ctgggagggc tgcgcgagca tgagcctgct tgagctcgag gcggtcaaca
     3721 ccttctacgg cgactcccac atcctgttcg atgtgtcgat gcgcgtggag cagaacgagg
     3781 ttgttgctct gctcggccgc aacggtgccg gcaagaccac gacactgcgc accatcatgg
     3841 gcgtcctcgc gccaggcagc ggcacgatca ggctggacgg aaagccgatc ggcggtctgc
     3901 cgccctatgt gatcgcgcgc atgggcatgc agctcgtgcc ggaggaacgc ggcgtcttcg
     3961 gcggcctgac cgtggaggag aacctgcgca tcgccgcgct caccgcacct gcgccctggg
     4021 gactcgaccg tatctacgcg atgtttcccc gcctcgccga acggcgccgc tcgcgtggcg
     4081 gcagcctgtc aggtggcgaa cagcagatgc tgaccatcgc ccgtgcactg atccgcgacg
     4141 cgcgcatcat cctgctggac gaaccgttcg aaggtctggc gccgctgatt gtgcgcgacc
     4201 tcgtgcggat cagccgcgag cttgcggcgc aggggcgcac gatcgttgtg gtggaacaga
     4261 acgtggtggc ggcgctcagc ttcgcggacc gcgtgtatgt cctgaacaac gggcacgtgg
     4321 tctacgaagg tacgcctgcc tcgctgcacg ccgaccccga ggtgatgaag agtcatctcg
     4381 gggtctgaag cggggtcagt agtggcccca gtagccaggg gcgccaaaca gcaggaacag
     4441 gacgagcagc agcaggatca ggccaaaccc gccgccatag tgagcgccgc cgtagtagct
     4501 ggaccggtag ccgtagtatc cgccgccgcc aaacagcagc acgatgatca ggatcaatag
     4561 taacaggccc atgatgcagt gccttttctc tgtggtgcca accaatgt
//
LOCUS       DATGEM010000056         6106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1236365, whole genome shotgun sequence.
ACCESSION   DATGEM010000056 DATGEM010000000
VERSION     DATGEM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236365"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="VKQ27_01615"
     CDS             complement(<1..2313)
                     /locus_tag="VKQ27_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HLY87658.1"
                     /translation="MTGTLPEHFARWFATQGWAPRRHQLAMLDAARAGESVLLIAPTG
                     GGKTLAGFLPTLVALAETPQDSLHTLYISPLKALATDIARNLMRPVEEMRLPVSIETR
                     TGDTPANRRARQREAPPNILLTTPESLAVLLSLPDAASLFAGLSCVVMDEVHALAGTK
                     RGDQLALCVSRLMTLAPICRRVGLSATVAHPAAIQAYVGAERVVEEPDGAPPELAMML
                     PEGELSWAGHMGLEAAPRVMERIRDAGMTIVFVNTRAQAELMFQALWRLNEPTLPIAL
                     HHGSLEVEQRRKVEAAMAAGKLRAVVATSSLDLGIDWGGVDQVIQVGAPKGVSRLLQR
                     VGRANHRMDEASRAILVPANRFEVLECEAAMLGVEARELDGDPPRPGGLDVLGQHLLG
                     LACSAPFRPDDVYTEVCNAAPYAGLSRKDFDDVLTFVENGGYALAAYDRWRKLFRDSE
                     GRVHVRSERIARQHRMNIGTIVEAPLVKVRLVGKKRFGPLLGEIEEYFVNLLRQGDTF
                     MFAGRLLRFLRLHENVVECAEGGTGEPMVPAYEGGRLPLTTNLANRVRGLLQNPSSWS
                     LFPDGVQDWLKLQKARSRMPGRSDLLVETFPRGDRWYLVAYCFEGRNAHQTLGMLLTR
                     RMEREGYAPLGFVATDYVLGIWSSEQPRDVARLFDQDMLGDDLETWLAESSMLRRTFR
                     NVAVIAGLIERHHPGAEKTRKQVTVNSDLIYDVLRRHQPDHILLRATRADAAGGLTDL
                     SRIAGMLARVKGRITHMVLSRVSPLAVPVLL"
     gene            2405..3451
                     /locus_tag="VKQ27_01620"
     CDS             2405..3451
                     /locus_tag="VKQ27_01620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HLY87659.1"
                     /translation="MAHPSTWLRVLPQGLYCEPGGFFIDPTRAVDSAVITHAHSDHAR
                     PGHRAVLATADTLALMRARMGEERAGTTQQALAWGETVDLGGVRVWLQPAGHVLGSAQ
                     VAMEYQGSRAVISGDYKRTADPTCAGFIPVPCDVFVTEATFALPVFRHPPPDQEIARL
                     LASVALFPDRTHVVGCYALGKCQRVIALLRAARWDGPIWLHGALASMCEVYEARGIAL
                     GDLRPATSAAKGELVGAIVLAPPGSVTDRWARRMADPVVAVASGWMRVRQRAKSRGVE
                     LPLVISDHADWDELNATLDDVGAPEVWVTHGREEALIHAAGTRGIEGRALRLVGYGEE
                     DEAEDPVEGEAVSP"
     gene            3482..3979
                     /locus_tag="VKQ27_01625"
     CDS             3482..3979
                     /locus_tag="VKQ27_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY87660.1"
                     /translation="MTDPLTFTFADDGAIPNSKLPLLVYRQAVPADPAAIERIFTANR
                     WPAAWRDSVHPFHHFHSDAHEVLGVASGSAKVRFGGPEGEVLDVSAGDVVVVPAGVGH
                     CNEGKSADLLIVGAYPDNTARRDLRRGNPSEHDEVIRNIAAVAMPNADPVGGHRGPLV
                     RIWSA"
     gene            complement(4079..4654)
                     /locus_tag="VKQ27_01630"
     CDS             complement(4079..4654)
                     /locus_tag="VKQ27_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87661.1"
                     /translation="MSVTEAVRDKPKLLNELPQRLHHHAYVVRDQEKNRQFFEDLLGI
                     PLVATWCESHHNSWLNREVAMCHTFFAMGDGGALAFFAFADPDIYKLVIAEKPPVVGN
                     FDHVAFKVSDKAYDEIVGRLKRAGEKFRETDHGYCRSVYATSPDGLIVEFTVDPPGVE
                     KIDAIRRADAHSELARWVAGDHRVNNDLRPH"
     gene            4733..6022
                     /locus_tag="VKQ27_01635"
     CDS             4733..6022
                     /locus_tag="VKQ27_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87662.1"
                     /translation="MHHRRLWICAFLFTLTLINYTDRVALSVAVTPISAEFHLTKVEM
                     GYLLSSFLWMYVICLIPVGLLVDRFGGKVVNAAGIGIWSTATVLTGFTSGFASMAATR
                     IVMGMGESTSWPACNRVIREWFPASERGFANAVFGAGAAAGPAIGAVVVSAVVAWLGW
                     RAGFLVAGGIGFVWLASWLLAFDRPDRVGWLHPAERDRILRERDGAARNSAGQPASPL
                     LHLLTLRSVWGLFLTQGCEVYGGYMLLTWLPNYLQSAKGLSVLNAGMLTAVPFGAATV
                     IAVALGFLSDRLLSHDAVHAGRRRTMLAVMMVSAASILLIPFIDDLWLIVLILAVARS
                     MGAAGSALNFALVTDLVRNPADIGKVTSITVLGGNSFGMMGPIVTGYVVGLTGSFNGT
                     FAVAGVLALIGAVATLTMTRRPIEAYGDSLPAAAQPA"
BASE COUNT         1004 a         1979 c         2006 g         1117 t
ORIGIN      
        1 caacagcacc ggcaccgcca gtggtgagac gcgcgacagc accatgtgcg tgatccgccc
       61 cttcacccgt gccagcatcc cggcgatgcg ggaaaggtcc gtcagcccgc cggccgcgtc
      121 ggcacgggtg gcccgcaaaa ggatgtggtc cggctggtga cggcgcagca cgtcgtagat
      181 cagatccgag ttcaccgtga cctgtttgcg ggtcttctcg gcgcccggat gatggcgctc
      241 gatcaaacca gcaatcaccg cgacgttgcg gaaggttcgc cgcagcatcg agctttccgc
      301 gagccatgtc tccagatcgt cgcccagcat gtcctggtcg aacaacctgg ccacatcgcg
      361 cggctgttca gaggaccaga tgcccagcac gtagtcggtg gccacgaagc cgagtggtgc
      421 atatccctca cgctccatcc gccgggtcag cagcatgccc aacgtttgat gcgcgttgcg
      481 gccttcgaag caatacgcca ccagatacca gcgatcgccg cgcggaaatg tctcgaccag
      541 caggtcactg cgccctggca tgcgcgagcg cgctttctgc agcttcagcc agtcttgcac
      601 gccatcggga aacagcgacc agctcgacgg attctgcaac agaccgcgca cgcggttcgc
      661 gaggttggtc gtcaacggca gccgtccacc ttcatacgcg ggcaccattg gctcgccggt
      721 gccgccttca gcgcattcta caacgttctc gtgcagccgc aggaagcgca gcagccgtcc
      781 ggcgaacatg aatgtatcgc cctgccgcag caggttgacg aagtattcct cgatctcgcc
      841 gagcagaggg ccgaaccgct tcttcccgac gaggcgcacc ttcaccagag gcgcctcaac
      901 gatggtgccg atgttcatcc ggtgctgcct tgcgatgcgc tcggagcgca catgcacgcg
      961 tccctcgctg tcgcggaaca gtttccgcca gcggtcatag gccgccagtg cgtagccgcc
     1021 gttctcgacg aacgtcagca cgtcatcgaa gtccttgcgc gatagccccg cgtacggtgc
     1081 agcgttgcat acttccgtat agacatcgtc cgggcggaat ggcgctgaac acgcaagccc
     1141 aagcaggtgc tgtcccagca cgtccagccc gccgggccgg ggcggatcgc catccaattc
     1201 gcgcgcctcc acgcccaaca tcgcggcctc gcactccagc acctcgaacc ggttggccgg
     1261 caccaggatc gcgcgcgatg cctcgtccat ccggtggttg gctcgcccga cgcgctgcag
     1321 tagccgggat acgcccttgg gcgctccgac ctggatcacc tgatcgacgc caccccagtc
     1381 gatgccgaga tccagcgacg acgttgccac cacggcgcgc agctttcccg ctgccatcgc
     1441 cgcttccacc ttacgccgct gctctacctc cagggacccg tggtgcagcg cgatcggcag
     1501 cgtcggctcg tttagccgcc acagcgcctg gaacatcagc tcggcctgag cccgcgtgtt
     1561 gacgaacacg attgtcatgc ccgcgtcgcg gatccgctcc atcacccttg gtgccgcttc
     1621 cagccccatg tggccggccc aggacaattc cccctccggt aacatcatcg ccagttcggg
     1681 aggcgcgccg tccggttcct ccacaacgcg ctcggcgcca acgtaggcct ggattgccgc
     1741 tggatgcgcg acggtcgctg acagacccac acggcggcat atcggcgcca gcgtcatcag
     1801 gcgcgacacg cacagcgcca actggtcgcc acgtttggtg ccagcgagcg catgcacctc
     1861 atccatcacc acacaggaca atccagcgaa cagcgacgcc gcatccggaa gcgacaggag
     1921 cacggcgagg ctctccggcg tcgtcagcag gatgttgggc ggagcttccc gctgccgcgc
     1981 acgccggttg gctggtgtgt ccccggtgcg ggtctcgatt gacaccggca gccgcatctc
     2041 ctcgactggc cgcatcaggt tgcgggcgat atcggtggcc agcgccttga gcgggctgat
     2101 gtacagcgta tgcaggctgt cttgcggcgt ctccgccaac gcgaccagcg tcgggagaaa
     2161 gccggcgagc gtcttgccgc ccccggtggg agcgatcaac agcacgcttt cgccggcccg
     2221 cgcggcgtcc agcattgcga gctggtgccg ccgtggcgcc cagccctggg tcgcgaacca
     2281 gcgcgcaaag tgttcgggta atgttcccgt catcggttca ggtaagcaca tcccataggg
     2341 cgaacgccac cctgccaaat cgccggatgt ggcatgcagg cccgcgatcc accacattgc
     2401 ctctgtggca catccgtcga catggcttcg cgtcctgccg cagggactgt attgcgagcc
     2461 aggcggcttc ttcatcgatc cgacccgcgc ggtggatagc gcggtgatca cgcatgccca
     2521 ctccgatcac gcgcgtccgg gacaccgcgc cgtgctggca acggccgaca cgctggcgtt
     2581 gatgcgtgcc cggatgggcg aggaacgcgc tggcacaaca caacaggctc tggcctgggg
     2641 cgagaccgtc gatcttggcg gtgttcgcgt ctggctgcag cccgccgggc atgtcctcgg
     2701 aagcgcgcag gtcgcgatgg agtatcaggg ttcccgtgct gtcatcagcg gcgattacaa
     2761 gcggaccgcc gatcccacct gcgccgggtt cattcccgtc ccatgcgacg tatttgtcac
     2821 cgaggcgacc ttcgcgctgc cggtgttccg gcacccgcct ccagaccagg agatcgcccg
     2881 gctgttggcg agcgtcgcgc tgtttcccga tcgcacgcac gtcgtcggct gctacgcgct
     2941 gggcaagtgc caacgcgtga tcgcactgct gcgtgccgcg aggtgggacg ggccgatctg
     3001 gctgcatggg gccctggcct cgatgtgcga ggtctatgag gcacggggca tcgcgctggg
     3061 cgatctgcgg ccggcaacct cggcggcgaa gggcgagctg gttggagcga tcgtgctggc
     3121 gcctcccggc tcggtgaccg atcgctgggc gcgccggatg gccgatccgg ttgtcgcggt
     3181 ggcgtcaggc tggatgcgcg tacggcagcg ggcgaagtcg cggggggtgg aactgccgct
     3241 ggtgatctct gaccatgccg actgggacga gctgaatgcg acgttggacg acgtcggcgc
     3301 gccggaagtt tgggtgacgc atgggcgcga ggaagcgctg attcatgcag ccggcacgcg
     3361 cgggatcgag ggacgggcgc tgcgtcttgt cggctacggc gaggaggacg aggcggagga
     3421 cccggttgag ggagaggcgg tcagtccgta gactgagccg gacagcagcg gagaagttgc
     3481 catgaccgac cccctgacct ttaccttcgc cgatgacggc gcaatcccga actcgaagct
     3541 gccattactc gtgtatcgac aggctgtgcc agcggatccg gctgcgatcg agcgtatctt
     3601 caccgcgaac cgctggcccg cggcgtggcg tgattctgtc catccgtttc atcatttcca
     3661 cagtgacgcg catgaggtgc tcggtgtggc cagcggcagc gcgaaggtgc ggttcggtgg
     3721 ccctgaaggc gaggtcctgg acgtcagcgc gggcgatgtc gtcgtggtgc ccgctggcgt
     3781 cggtcactgc aatgagggca agagcgccga tctgctgatc gtgggcgcgt atccagacaa
     3841 tacggcgcgg cgcgatctac ggcgcggcaa tccgagcgag catgatgagg tgatacggaa
     3901 catagcagcg gtggcaatgc cgaacgccga tccggtcggt ggccaccgcg ggccgctcgt
     3961 gcgcatctgg agcgcatagc cggggcgtta cgtcattgcg aggagcaacg cgacgaagca
     4021 atctactccg gtattgcttc gccgctccgc tcctcgcgat gacaatcggt gcgcggtgct
     4081 agtgcggacg caggtcgttg ttgacgcgat gatcgccagc cacccaccgg gccagctcgg
     4141 aatgcgcatc ggcccgccgg atcgcgtcga tcttctccac cccaggcgga tcgacggtga
     4201 actcgacgat caaaccatcg ggtgacgtcg catagaccga cctgcaataa ccgtggtcgg
     4261 tctcgcggaa tttctcgccg gcgcgcttca gccggccaac gatctcgtca taggccttgt
     4321 cggaaacctt gaacgcgacg tggtcgaaat tgccgaccac aggtggcttc tccgcgatca
     4381 ccagcttgta gatgtccgga tcggcaaatg cgaagaacgc gagcgccccg ccgtcgccca
     4441 tggcgaagaa ggtgtggcac atcgccacct cccggttcag ccaggaattg tggtggctct
     4501 cgcaccaggt cgcgaccaga ggaatgccca gcaggtcctc gaagaactgc cggttcttct
     4561 cctggtcgcg cacgacatag gcgtggtgat gcagccgctg cggcagttca ttcagcagct
     4621 ttggcttgtc ccgaacagct tcagtcactg acatggcgac gtcctcctgt tgctgggcaa
     4681 agcttgcgct tccggtgcgg ccctcgcaag ctgatcatcg ggggaaaccg taatgcatca
     4741 tcgtagactt tggatctgcg ccttcctgtt cacactgacg ctgatcaact acaccgaccg
     4801 ggtcgcactg tccgtcgcgg tcacaccgat ctccgctgag tttcatctaa ccaaagtgga
     4861 gatgggttat ctgctttcgt cctttctatg gatgtatgtg atctgcctga ttccagttgg
     4921 tcttctggtg gatcgcttcg gcggcaaggt ggtgaacgct gcgggtatcg gcatctggtc
     4981 gaccgccaca gtactgaccg ggtttacctc gggcttcgct tccatggcgg cgacacgcat
     5041 cgtgatgggc atgggtgagt ccacgagctg gcccgcatgc aatcgggtga tccgcgaatg
     5101 gttcccggca tctgagcgcg gctttgccaa tgcggtgttt ggcgctggcg cagcagcggg
     5161 gccggcgatc ggcgcggtgg tggtgtcggc tgtcgtcgcg tggctcggct ggcgcgccgg
     5221 cttccttgtg gccggcggca tcgggttcgt gtggctggcg tcgtggctgc tggcgttcga
     5281 cagaccggac cgcgtcggct ggctacaccc tgccgagcgc gatcgcatcc tgcgcgaacg
     5341 tgacggtgca gcacggaact cggcgggaca accagcgtcg ccgctactgc atcttctaac
     5401 gctgcgcagc gtttggggac tgtttctgac ccagggatgc gaggtctatg gcggctatat
     5461 gctgctgacc tggctgccga actatctgca aagcgccaag gggttgtcgg tgctgaatgc
     5521 tggcatgctg actgcggtgc cattcggcgc ggccaccgtg atcgcggtag cgctcggctt
     5581 cctgagcgac cgcctgttgt cgcacgacgc ggtgcatgcg ggacggcggc gcacgatgct
     5641 ggccgttatg atggtgagcg cggcgagcat tttgctgatc ccgttcatcg acgatctctg
     5701 gctgatcgta ctgatcctgg ccgtggcgcg ctcgatgggc gcggcgggca gcgcactgaa
     5761 cttcgcgctg gtgaccgatc tggtgcgcaa tcccgcggat atcggcaagg tgacgagcat
     5821 cacggtactt ggcggcaact ccttcggcat gatgggtccg atcgtcactg gttacgtggt
     5881 cggactgacc ggcagcttca atgggacgtt cgcggtagcg ggcgtgctgg cgctgattgg
     5941 cgccgtcgcg acgttgacca tgacgcgccg gccgatcgaa gcctatgggg actcgctgcc
     6001 ggccgcagca cagccggcat agcgtcattt gcgtgggccg cgccggagca attcagcggt
     6061 actgctcgat ccggcgtagc ggggcaactc gtccaggatc tcgaac
//
LOCUS       DATGEM010000057         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_438571, whole genome shotgun sequence.
ACCESSION   DATGEM010000057 DATGEM010000000
VERSION     DATGEM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438571"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VKQ27_01640"
     CDS             <1..838
                     /locus_tag="VKQ27_01640"
                     /inference="COORDINATES: protein motif:HMM:NF019221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BBP7 family outer membrane beta-barrel protein"
                     /protein_id="HLY87663.1"
                     /translation="RQGFSGGPKIRLTYHDDSGYGFELTYFNVFNQTGTETIGPDNPA
                     SWFLMRAPGGFWQTQDFPYQAMAWSNTTNLYSAEANMRLELLPRVTALAGFRWLQLND
                     NLQGTLTPSDRTAPSWKTSCPLCNVFQITPDGPIGNLPPFWNGGTTNNLYGVQVGIDA
                     DILRFDRFSLGGRATIGLFDNNARQSTGVSIEKVVYPSTATANGAAFVSEAGLQLKYR
                     VTNGLSLKAGYEVLWLVGVALAPGQIEKTHAAPADVQALGVNHGSNVLFQGATFGLEY
                     SF"
     gene            924..1763
                     /locus_tag="VKQ27_01645"
     CDS             924..1763
                     /locus_tag="VKQ27_01645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY87664.1"
                     /translation="MIIDSQVHAYEANTPKRPWHSVPNWPDHVTGDEMVAAMDKLGID
                     GAIFISAFGLYQYDASYAVEVQQKHPDRFAIVKPVNPDDPAVADVIAEWKKVPGAVGI
                     RIMLPPESGRAADDPGLDRIARAAVRYDLPVNVLFWGNVDVGTALIDRHPDTRFIIDH
                     LAIMQPRVPPAPPQPWADLPKVLDLAKRKNAVIKVSGACTLSQKPFPYPDIWDPLARV
                     FDAWGLDRCLWGTDWTRAYAVVNFEQAVEPFLKTDRLSDSDRAILMGGACAKAYGWSP
                     RKA"
     gene            complement(1771..2394)
                     /locus_tag="VKQ27_01650"
     CDS             complement(1771..2394)
                     /locus_tag="VKQ27_01650"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HLY87665.1"
                     /translation="MILIGQYDSPFVRRVAISLRVSAIAYQHDTRSVFADFDAMRLVN
                     PLGRIPSLVLDDGDVLIDSAAILDWLDQSVGPERALIPAVGMDRRRMLRRMALATGVI
                     DKAGAAAYERLIRPEAFRWPQWIERCLTQAAGGLEALAAETWPARDRLDQAEITTACM
                     LRYLRIAVPDVLPAGRYPSLDVLSARCESRPEFIATCPADYAVPRDG"
     gene            2511..>2873
                     /locus_tag="VKQ27_01655"
     CDS             2511..>2873
                     /locus_tag="VKQ27_01655"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HLY87666.1"
                     /translation="MADDSRGDLGARTRFMRITPATGENLRAVWKLVEPALPAILDEF
                     YKHVSAEPSLRSLFGDTIPRLKQAQTRHWQKMFAGSFDADYYEGVRAIGLVHHRIGLA
                     PRWYIGGYNFVLEHLTELA"
BASE COUNT          555 a         1001 c          824 g          493 t
ORIGIN      
        1 ccgccagggc ttttccggcg gcccgaaaat acgactgacc taccacgacg attctggcta
       61 tggcttcgag ctaacgtatt tcaatgtgtt caatcagacc ggcacagaga caatcggccc
      121 ggacaatccg gcgagctggt tcctcatgcg agcgcccggc gggttctggc agacgcagga
      181 cttcccgtac caggccatgg cgtggagcaa caccacgaat ctctacagcg ccgaagccaa
      241 catgcgcctg gagctattgc ctcgggtgac cgcgctcgcc ggtttccgct ggctgcagct
      301 gaatgacaat ctgcaaggca cgctgacccc ttcggaccgc accgcgccga gctggaaaac
      361 cagttgtccg ctttgcaatg tatttcagat caccccggat ggtccgatcg gcaacctgcc
      421 gccattctgg aacggcggca cgacaaacaa cctttatggt gtccaggtcg gtatcgacgc
      481 ggacatactg cggtttgatc gcttctccct cggcggacgc gcaacgatcg gcctgttcga
      541 caacaatgcc aggcagtcaa ccggcgtcag catcgagaag gtcgtatatc cctcaaccgc
      601 caccgccaac ggcgccgcct tcgttagcga agccggtttg cagttgaagt accgggtgac
      661 caacgggcta tccttgaaag caggctacga agtgctgtgg ctcgttggcg ttgccttggc
      721 gcccggtcag atcgagaaga ctcacgcagc gcccgctgat gtgcaggcgc tcggcgtcaa
      781 tcacggatcg aatgtgctct tccagggtgc cacgttcgga ttggaatatt cgttttagta
      841 tcggactacc cgcgcttcgg tccatcctct ggacactccc catcaccgtc gccaagcttc
      901 cggcacacag aggagcgtcc ggaatgatca tcgactccca ggtccacgcc tacgaagcca
      961 acacgccgaa gcggccctgg cacagcgtgc ccaactggcc agatcacgtc actggcgacg
     1021 agatggtcgc cgcgatggac aagctcggca tcgacggcgc gatcttcatc tctgcgttcg
     1081 gcctctacca atatgacgca agctacgcgg tggaggtgca gcagaagcac cccgaccgtt
     1141 tcgccatcgt caagccggtc aaccccgacg accccgccgt cgccgacgtc atcgccgaat
     1201 ggaagaaggt ccccggcgca gtcggcatcc gcatcatgct gcctccggaa tctggccgtg
     1261 ccgcggacga cccgggcctc gaccggatcg cgcgcgcggc cgtgcgctac gatcttcccg
     1321 tcaacgtcct gttctggggc aacgtcgacg tcggcaccgc gctgatcgac cgccaccccg
     1381 acacgcgctt catcatcgac catctcgcca tcatgcagcc gcgcgtgccg cccgctccgc
     1441 cgcagccctg ggccgacctg ccgaaggtgc ttgacctcgc caaacgcaag aacgccgtga
     1501 tcaaggtcag cggcgcctgc acactctcgc agaagccctt cccgtacccc gacatctggg
     1561 acccgttggc ccgcgtgttt gatgcctggg gccttgatcg ctgcctctgg ggcaccgact
     1621 ggacgcgcgc ctatgccgtc gtcaacttcg aacaggccgt cgaacccttc ctgaagaccg
     1681 accgcctcag cgacagcgac cgggccattc tgatgggcgg cgcctgcgcc aaggcgtacg
     1741 gctggtcacc gcgaaaagcc tgagccgaga tcatccgtcc cgcggcactg catagtccgc
     1801 gggacaggtg gcgatgaatt ccggccggct ttcgcagcgc gctgacagca cgtccagcga
     1861 cgggtaccgg ccggccggaa gcacatcggg aaccgcgatg cgcagatagc gcagcataca
     1921 ggcggtcgtt atctccgcct gatccagccg gtcccgcgcg ggccaggttt ccgccgccag
     1981 tgcctccagc ccacccgccg cctgcgtcag gcagcgctcg atccattgcg gccagcgaaa
     2041 agcctccggc cggatcagcc gctcgtaagc cgcggcacca gccttgtcga tcacgcctgt
     2101 cgccagcgcc atccgccgca gcatacgccg cctgtccatc cccaccgccg gaatcagcgc
     2161 gcgttccggg ccgacagact ggtccagcca gtccagtatc gccgccgaat cgatcagcac
     2221 atcgccatcg tcgagcacga gcgatggtat gcgtccaagc ggattgacca gtcgcatcgc
     2281 atcgaagtcc gcgaacaccg agcgcgtgtc gtgctgatag gcaatcgccg acacgcgcag
     2341 cgagatcgcc acacggcgca caaagggaga gtcgtattgg ccgataagta tcatgactgc
     2401 ctcctccgca tgacgaatcc agagaacgcc aacttgcggc cctttaccaa aaattttctg
     2461 ctcgctcccg acaatgcgcg ccacgtccct caaatgccac ggagtgactt atggccgatg
     2521 acagccgcgg cgatcttggc gccaggaccc gattcatgcg gatcaccccg gcaacagggg
     2581 aaaacctccg cgccgtctgg aagctggtgg agcccgcgct ccctgccatc ctcgacgagt
     2641 tctacaagca cgtcagtgcg gaacccagcc tgcgctcgct gttcggcgac acgatcccgc
     2701 gtctcaagca ggcccagacc aggcattggc agaaaatgtt cgcgggtagc ttcgatgccg
     2761 actactacga aggcgtccgt gcaatcggcc tcgtgcatca tcggatcgga ctcgcgccgc
     2821 gctggtatat cggcggttac aacttcgtcc tggagcactt gaccgaactg gcc
//
LOCUS       DATGEM010000058         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1694521, whole genome shotgun sequence.
ACCESSION   DATGEM010000058 DATGEM010000000
VERSION     DATGEM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694521"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1049)
                     /locus_tag="VKQ27_01660"
     CDS             complement(186..1049)
                     /locus_tag="VKQ27_01660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY87667.1"
                     /translation="MAALIPKPKGLERQYGDQFADAAIVAAYRHRPAYAPEVFTFLRD
                     LVIGQPRSVLDLGCGTGDVARPLAALVDTVDAVDPAHRMIEHGRSLPGGDRSNLRWIL
                     GYAEEVQLPRGTYGLVTAGESLHWMDWSRLLPRLNGLLVPGGFLAIVERNVAPNPPWW
                     TEVLVPIQQYTTNRDFQPYSTIAELEQRGLFEPCGVHQAAARRFQQSVESYIESIHSR
                     NGFSRDRLTPEAAAAFDAEVRRLLIARYPHGTLAFDIVSQITWGRPVGNQTSEVRSQP
                     DHATRPGSSLS"
     gene            1094..2242
                     /locus_tag="VKQ27_01665"
     CDS             1094..2242
                     /locus_tag="VKQ27_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY87668.1"
                     /translation="MIDGTGGAVQRDRALFVEDGQIVGIEAAGQVPADAELVDLSDLT
                     VMPGLIDCHVHLVFSSSAYALGDVLAENDQQLLLRGVAAARQALGAGITTVRDLGGRG
                     GVTFRLRDAIAAELIAGPRILAAGSPITITGGHCHFLGVEADGEPAVRAAARLQLKSG
                     ATCLKIMATGGRMTPGTNPGMAQYSVAELRAAVDEAHRAGVPLAAHALGTPGIRNATA
                     AGVTTIEHCNWLGPNGTVDFDESVAMQMAQQSTGVVPTLVPLKRSAPALREQIMDCMR
                     RMATLGVPIVAGTDAGVSLTPFDSLPGELEIYVADMGLSPLQAIQAATGTAARLLGLD
                     GTLGTLAAGRAADLVAVAGDPSVQIEDVRQVRTVIKGGRTVVDNGIVL"
     gene            complement(2270..>2450)
                     /locus_tag="VKQ27_01670"
     CDS             complement(2270..>2450)
                     /locus_tag="VKQ27_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87669.1"
                     /translation="ENYPLSVGLRYFWVNPGDSLPKDHLLMAASIIMTLPVIILFFIG
                     QRSFVRGVVMSGIKG"
BASE COUNT          422 a          778 c          823 g          427 t
ORIGIN      
        1 cgggtcccat ccgaagtgac tgtcatcctg agcctgcgaa ggatccgtac tcttcgcctc
       61 gcgacaaggg atccttcgtc gttcctcctc aggatgacaa ttcgctgtca taggactcgg
      121 aactttcgga atgcaccact aagccgtcgg cgggcgggac tgttgtcatt ggatggatcg
      181 ctgtgtcatg acagactact gcctggccgc gtggcgtggt ccggctgact tctgacttct
      241 gatgtctgat ttcccacggg tcgaccccag gtgatctgcg agacaatgtc gaatgcaagt
      301 gtgccatgtg ggtagcgggc gatcagcaat cgccgcactt cggcatcaaa ggccgcggcg
      361 gcctcgggcg tcaaccggtc gcgcgaaaag ccgtttctgg aatggatcga ctcgatgtag
      421 ctttcaactg actgctggaa tcgccgcgcg gcggcctggt gtactccgca cggttcgaag
      481 agtccacgtt gttccagctc cgcaatcgtg ctgtatggct ggaaatcgcg attggtggtg
      541 tactgctgga tcggcaccag cacttctgtc caccacggcg ggttcggcgc aacgttccgt
      601 tcgacaatcg ccagaaaacc gcccggtaca agtaggccgt tcaaccgtgg cagcaagcgg
      661 ctccagtcca tccagtgcag gctctcgccg gccgtgacca gtccatacgt gccgcgtggc
      721 agctgcacct cctccgcata gccgaggatc cagcgcaggt tcgatcgatc gccacccggc
      781 agactccggc catgctcaat catccggtgg gccggatcaa cggcgtcaac tgtgtcgacc
      841 agcgcggcga gtggtcgggc gacatccccg gtgccacagc cgagatccag cacgctgcgc
      901 ggctggccaa tgaccaggtc gcgcaggaag gtgaacacct caggcgcata ggccggccga
      961 tggcggtagg cagccacgat cgccgcatcg gcgaactggt cgccgtactg gcgctcgagg
     1021 cccttcggct tgggaattag agcggccacg cgcgcgatat cctgcggggg tgaaggtgtt
     1081 ccgagcgagc cggttgatcg atggcacagg cggcgcggtg cagcgcgacc gggcgttgtt
     1141 tgtcgaggac ggacagattg tcgggatcga agcggcaggt caggtgcctg ccgacgcgga
     1201 gctggtcgac ctgtccgacc tgacggtcat gcccgggctg atcgactgcc acgtgcacct
     1261 ggtcttcagc tcgtcggcgt atgcgctcgg cgacgttctg gcggagaacg accagcagct
     1321 gttgctgcgc ggggtcgccg cggctcgcca ggcgctgggc gccggcatta cgacggtgcg
     1381 cgatctcggt gggcgcggcg gcgtaacctt ccgcttgcgc gatgccatcg cggccgagct
     1441 catcgccggt ccgcgcatcc tggcggccgg atcgccgatc acgattactg gcgggcactg
     1501 ccatttcctc ggcgtcgagg ccgacggcga accagcagtc cgcgcggcag cccggctcca
     1561 gctcaaatcg ggcgcgacgt gcctcaagat catggcgacg ggcgggcgta tgacgcccgg
     1621 gaccaacccg ggcatggcgc agtacagcgt tgccgagctg cgcgccgcgg tggacgaagc
     1681 gcaccgtgcc ggcgtccccc tggccgctca tgcgctgggt acgccaggca ttcgcaacgc
     1741 gactgccgcg ggcgtcacga ccatcgagca ctgcaactgg ctgggtccga atggaacggt
     1801 cgacttcgac gagtcggtag cgatgcaaat ggcccagcag agtacgggcg tcgtgccaac
     1861 tctggtacct ctgaagcgat ctgcgccagc attgcgtgag cagattatgg actgcatgcg
     1921 gcggatggcg acgctcggcg tgccgatcgt cgccggcacg gacgcgggcg tctcgttgac
     1981 gccgttcgac agcctgccag gcgagctgga gatctacgtc gccgatatgg ggctgtcgcc
     2041 gctccaggcg atccaggcgg ccaccggcac cgccgcccga ctgcttggcc tcgatggcac
     2101 gctcggaacg ctcgccgccg gccgagccgc ggacctggtg gcggtagctg gcgatccgtc
     2161 agtgcagatc gaggacgtgc gccaggttcg cacggtcatc aagggtggcc ggaccgtcgt
     2221 cgacaacgga atcgtgctgt gacgactgac agctaccgac ttcggctgct cagcccttga
     2281 tgccagacat cacgacgccg cggacgaacg aacgctggcc gatgaagaac agaatgatca
     2341 ccggcaacgt catgatgatc gaggcggcca tcagcagatg gtccttcggc aggctgtcgc
     2401 cgggattgac ccagaagtaa cgcagcccga cgctgagcgg gtagttctcc
//
LOCUS       DATGEM010000059         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1694075, whole genome shotgun sequence.
ACCESSION   DATGEM010000059 DATGEM010000000
VERSION     DATGEM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694075"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1009)
                     /locus_tag="VKQ27_01675"
     CDS             complement(116..1009)
                     /locus_tag="VKQ27_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY87670.1"
                     /translation="MGEQIRSAIPSFLLYGEHGAEPPFIHIETVAARAGALNWTIDAH
                     RHDALGHVLLIKGGGGRFLLDGVVRQFGPGSAIFVPSKLVHAFYFLPDTDGSVLTLSD
                     SLLELAADALVESDLSGDLAGALLVDLAQDPTLFEQIAASLARISAELAQPQLGQLSA
                     IRTHVALILLAMVRGGRFHGEQSLYSSQDLRLIEKLRRRIGQSLGEMPGVGDLACELG
                     VTAARLNAACRRVAGCSTLHLLHAAQLAEAKRALLYTEQPISEIAYSLGFEDPAYFSR
                     FFSRRTGRTPSAFRALHIRVG"
     gene            1117..2283
                     /locus_tag="VKQ27_01680"
     CDS             1117..2283
                     /locus_tag="VKQ27_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HLY87671.1"
                     /translation="MKRKVQVGIIGAGPAGLLLAHLLKRAGIEALVVDNRSRDEIEST
                     IRAGVLEQWTVDLLISLGLGERLKREAYFHDGITLRFNRGSHHLDFPELTNGKRVTVY
                     AQHEVLKDLIAALPEDMIQFGVKEVALTKLDTDRPAIRTAEDEIECDFIAGCDGFHGP
                     SRRAIPKSAIRTYETVYPFGWLGILTEAPPSHHELVYARHERGFALLSTRSSTVQRLY
                     LQCAPKDDLNQWSDDRIWSELRQRLETDEDWSLTEGTIFQKNIVSMRSFVCETMRWGR
                     LFLAGDAAHIVPPTGAKGLNLAAADVVVLSRALIAWYRDHGTDLLDRYAPTCLARIWK
                     GERFSWYMTTLLHRNDLEEEFVQRIHMADLEYLVGSHAAQQALAENYVGLPMAV"
     gene            2457..2666
                     /locus_tag="VKQ27_01685"
     CDS             2457..2666
                     /locus_tag="VKQ27_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLY87672.1"
                     /translation="MPNGTVKWFNSTKGYGFIAPDNGGADIFVHISAVERAGLSGLND
                     GQKVSFDEERDPKKGKSSAVNLKTL"
     gene            complement(2720..3091)
                     /locus_tag="VKQ27_01690"
     CDS             complement(2720..3091)
                     /locus_tag="VKQ27_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87673.1"
                     /translation="MEPSISPTNEVVVTGLVEWGLDDLEQSVNAMPTPVDRVVATAGL
                     QGGADLLVTDVGLPNGMNGRQLADAARELRPGLKVLFITGYAENALLSEGHLGRGMQA
                     MTKPFTMEELAVRLRDSLGEG"
BASE COUNT          682 a         1128 c         1038 g          580 t
ORIGIN      
        1 gtgggacgcc ttggcggcgt tcgatggggt aacgctacat cgaagccggg tgagccaggc
       61 tatccgaaag gtggaggcca cgataagatt tcgttataca aaccccttcg ccactttatc
      121 cgacgcgaat gtgcaaggcg cggaaagccg agggagtccg cccggtccgg cgtgagaaga
      181 agcgcgaaaa ataggccgga tcctcgaagc ccaggctata ggcgatctcg ctgatcggct
      241 gttcggtata gagcagcgcg cgcttggcct cggccagttg ggcggcgtgc agaagatgga
      301 gagtcgaaca gccggccacc cggcggcagg cggcattgag ccgggccgcg gtgacaccca
      361 gttcgcacgc cagatcccct actcccggca tctcccccag actttgcccg atccgccgac
      421 gcagcttctc gatcagccgc agatcctgcg acgaataaag cgactgctcg ccatggaagc
      481 gcccgccccg caccatcgcc agcaggatca gggcgacatg ggtgcggatc gccgaaagct
      541 gcccgagctg cggctgcgcc aactcggccg aaatgcgcgc cagcgaggcg gcgatctgct
      601 cgaacagcgt cgggtcctgc gccagatcga ccagcagcgc gccggccaga tcgccggaca
      661 gatccgattc caccagcgcg tcggcggcga gttccagcag cgagtcggac agggtcagca
      721 ccgacccgtc ggtgtcgggc agaaaataaa aggcatggac cagcttgctc ggcacgaaga
      781 tggcgctgcc cggtccgaat tgccgcacca cgccgtcgag caggaaacgc ccgccgccgc
      841 ccttgatcag cagcacatgg cccagcgcgt catggcggtg cgcgtcgatg gtccagttca
      901 gcgcgccggc ccgcgccgcg accgtctcga tatggatgaa aggcggttcg gcgccgtgct
      961 cgccatagag caggaaactc gggatcgcgc tgcggatctg ctcgcccatg cctcaccaaa
     1021 agtacaagag aaaacacgct tcatccattc cagcctcttg tagtggctgg atacaatgat
     1081 gatgcctcgc cagaaggcct atgggaagga aaagccatga agcgtaaagt gcaagtcgga
     1141 atcatcggcg ccggaccggc cgggcttctc ctggcccatt tgctcaagcg cgccggcatc
     1201 gaggcgctgg tggtggacaa ccgatcgcgc gacgagatcg aaagcaccat ccgcgccggc
     1261 gtgctggaac agtggaccgt cgatctgctg atttccctgg gcttaggcga acggctgaag
     1321 cgcgaggcgt atttccatga cggcatcacg ctgcgcttca accgcggcag ccaccacctg
     1381 gattttccgg aactgaccaa cggcaagcgc gtcaccgtct atgcccagca cgaggtgctg
     1441 aaggacctga tcgccgccct gcccgaggac atgatccaat tcggcgtcaa ggaggtcgcc
     1501 ttgaccaagc tggacaccga ccgcccggcg atccgcaccg ccgaggacga gatcgagtgc
     1561 gatttcatcg ccggctgcga cggcttccac ggccccagtc gccgggccat cccgaaatcg
     1621 gcgatcagga cttacgagac ggtctatccg ttcggctggc tcggcatcct gaccgaggcg
     1681 ccgccctcgc atcacgaact ggtctatgcc cgccacgagc gcggcttcgc cctgctctcc
     1741 acgcgatcgt ccaccgtgca gcgcctctac ctccaatgcg ccccgaagga cgacctcaat
     1801 caatggtccg acgaccgcat ctggtcggag ctgcgtcagc ggttggagac cgacgaagac
     1861 tggtcgctga ccgaggggac gatctttcag aagaacatcg tctcgatgcg cagcttcgtc
     1921 tgcgagacca tgcgctgggg ccgcctgttc ctggccggcg acgccgccca catcgtgccg
     1981 ccgacgggcg ccaagggcct gaacctggcc gccgccgacg tggtggtgct ctcccgcgcg
     2041 ctgatcgcct ggtatcgcga ccacggcacc gacctgctcg accgatacgc cccgacctgc
     2101 ctggcccgca tctggaaggg cgaacgtttc tcctggtaca tgacgaccct gctgcaccgg
     2161 aacgacctcg aggaggagtt cgtccagcgc attcatatgg ccgatctgga gtatctggtg
     2221 gggtcgcacg cggcccagca agcgctggcg gagaactatg tcggactgcc gatggcggtg
     2281 tgatcgcggc accgcagaga tagatttcgc gtcggggggc tcggttttgg ttgctattcg
     2341 gtaacaaaga cgtaaaagtt tccccgtcaa tcctgcctag aagacatcaa aatcgctcgg
     2401 cacttgatgc gcgtcgcact cgggcggcgc gctatcctca tctttggaga catcaaatgc
     2461 cgaacggcac tgtcaaatgg ttcaacagca ccaagggtta tggcttcatc gctccggaca
     2521 acggcggcgc tgacattttc gtccacatct cggcggtcga gcgcgctggc ctgtccggcc
     2581 tgaacgacgg tcagaaggtc tcgttcgacg aagagcgcga ccccaagaag ggcaagtcgt
     2641 cggcggtcaa tctcaagacc ctgtaatttg ctgagccggt ccgatggcgg gaaaccccgc
     2701 catcggattg gttacccgac taaccctccc cgagactatc cctcagccgg accgccagtt
     2761 cctccatcgt gaacggcttg gtcatggcct gcatcccgcg cccgagatgc ccctcgctca
     2821 atagagcgtt ctccgcatat cccgttatga acagcacctt cagccccggc cgcaattccc
     2881 gcgcggcgtc ggcgagctgg cggccattca tgccattcgg caagccgaca tcggtgacca
     2941 gcaaatcggc ccccccttgt agccccgctg ttgccaccac cctgtcaaca ggagtcggca
     3001 tcgcattgac agattgttcc agatcatcaa gcccccactc caccaagccg gtgacgacta
     3061 cctcattggt cggggagatc gaaggctcca cgcgaacgat ctcgccaatt tgagcgccag
     3121 caagcgctga tcatcgggga agcgggtatg gactattgat cgacgccacc tactacatgc
     3181 ggccgtggat gctgacgccc tgacgagatc gaagcgttgg cgctctgcgc gtaatgggtg
     3241 gcagagcacg gcgacaaggt gctgttaggt gccagcagcg atgtcatcgc gaaaatcgct
     3301 gccgtggtgc acgagcgcct gcggcctttc atcgccgagg agacgaacac gttcagtcag
     3361 cttttcactg gtaaggaacc agagagaaca atccggcccg ctatatcgcc ccgaccagcc
     3421 cccccccc
//
LOCUS       DATGEM010000060        11826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1255134, whole genome shotgun sequence.
ACCESSION   DATGEM010000060 DATGEM010000000
VERSION     DATGEM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11826
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255134"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VKQ27_01695"
     CDS             <1..1782
                     /locus_tag="VKQ27_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY87674.1"
                     /translation="TAQMNGADIIVDFLVRQNVPYLFGLCGHGNVGFLDAAFKAQNRI
                     RTISVHHEQSAGHMADAYFKVRHEPVATFTSCGPGSANLVVALAAAMMDSSAFFAITG
                     NVPTSQFNRQPFQETGRYFEGDFPSVIRPYVKRSYQPTRVDMVPLAVRQGWELMLSGR
                     PGPVNLDVPLNVFVEEAEITPPLSSARIDNRACGNPQALASAFDMLLAAARPVIIAGQ
                     GVLLAEASAELQQFAEQLAIPVATSPNGKGAINERHALSLGSIGRNGTYAANDATKNA
                     DVILSLGFTFDDRATSAWLDGYTLSVPPSRLIQIDIDPAELGRNYPAELPILGCARAS
                     LEHMNKLARGRKSANRDAWLSRLQRGREVWREYQGGLASSSQMPMRPERLMRALSNAL
                     PDNAIIASDVGVHHNWIIQLFEALRPRHLIHSWGFAAMGFATSGILGAKLAAPDRPCV
                     AVVGDGSFLMTPHALATAVEYDIPAVWVVWNNQGYCSIRDIQHGMFAGRELATSFQHD
                     SNRELYSPDFAALAKSFGVASHTVTHADEVEDSIKTAIATNRPYLIEVPVDREIRPIG
                     TGSWELPPLPQPEPNFLKALAAAGLSL"
     gene            complement(1913..3271)
                     /locus_tag="VKQ27_01700"
     CDS             complement(1913..3271)
                     /locus_tag="VKQ27_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY87675.1"
                     /translation="MRVLGTMAAIAGVPVVGLLALAGLGWAPVGPVAVAVGVTLLGAL
                     GLAVRWARDLDIVAESLRRLNADEATSRSSGIPVLPGLQRLVGELDRLGRRTAVRSAM
                     LEQLRRADGTVVERLPDPLIVLAADRSVRRANSAAREAFGTDMGAVLRHPQLRGAIDR
                     AFASGATEIAEVTLPVPAQREVYAAVVPMDPPIADGGRAVVVLSDRTRERAVERMRVD
                     FVANVSHELRTPLASLIGFIDTLRGPAADDPPAQQRFLGIMSEQAARMNRLIDDLLSL
                     SRIELSEHQAPSGKVDMRGLVARLVAGFEPRLKERSVKVELRMPDDVPPVTGDADQVT
                     QVLQNLLDNALKYGREGGTVRLEISVPSGRPGVLTTVTDQGAGIPREHLPRLTERFYR
                     VDTGRSRAAGGTGLGLAIVKHIVNRHRGQLSIESEEGIGTTVSVWLPQAVARAVASDA
                     AA"
     gene            complement(3276..3707)
                     /locus_tag="VKQ27_01705"
     CDS             complement(3276..3707)
                     /locus_tag="VKQ27_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HLY87676.1"
                     /translation="MRAREALEAPEALVLTMVLVAAADGGMTDREIGVMAGQVQTLPA
                     FKDFTSESLRVATDAAVKLLDEEDGLQHAARLMCGALGPKLRETAYALACEVVAASPV
                     ARQRTLEMLAFVRDELDLDPLIAAAIERGVRARHRRVDTGS"
     gene            complement(3759..6545)
                     /locus_tag="VKQ27_01710"
     CDS             complement(3759..6545)
                     /locus_tag="VKQ27_01710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HLY87677.1"
                     /translation="MARISLPDAPSVVAGHGRAAIFTADGELLLLPASEAAARLRGMA
                     PPLLVHAPATFRRLGLHGMPAFDLLELFAFVLPARPAAPTPRGLALMLDFEPPRGGLE
                     AEAALLPELTAALLQRLAQGRDIALNRDAAGLAARMGQVGWSWAPFVADALGQPDAAP
                     SGEALRVWKRLPEWEDVAPMPPPASHPVSEGEARSRLAAMLGPHAEQRPGQADYAGAA
                     AAAFAPRETRGDPHLVLAEAGTGTGKTLGYLAPASLWAEKNHGGVWISTFTRHLQRQI
                     ESELAHLFPDPNERRKRVVVRKGRENYLCLLNMEDAVSAAAPAIVVAMGLMSRWALVT
                     NDGDIQGGDLPGWLAELFGGATIYGLADRRGECIHAACPHWKRCFVEHTIRRARSAQL
                     VVANHALVMTQAAWGGLDDNLVPSRYVFDEGHHVFDAADGAFSAALSGMETAEMRRWL
                     LGAEGGRSRARGLKRRIDELVAARPTLEAPLEAALQAARALPGPGWHSRLASERPDIG
                     VESGRPNPTEDFLRLIRRQVLARTAGDEMARGSLECDVFPPGPDVGSAAERLARALAR
                     VAGPIATLRERLLARLDEEAEDMDAATRNRIEAAGRSLNRRLLIPLSAWQNMLRAIND
                     AQAEPGNRPNHIMFLRLDRVDGERDLDVGLHRHWLDPTIPFAATLALPAQGLLVTSAT
                     LRDAGDANPEFAWEAAEARVGAPHLPSPAIRAAVASPFDYAAQTRAFIVTDVITGDVA
                     QLAAAYRALFRASGGGALGLFTAIRRLQAVHARIAPDLEQAGIPLYAQHVDAMGNATL
                     VDIFRTEEESCLLGTDAMRDGVDVPGRALRLVVFEKVPWPRPDILHRERRVHLSDGDP
                     KGYDDRIARLRLRQAFGRLIRRATDRGVFVLLDRQTPSRLLSAFPAGVTVRRIGLAEA
                     VKETRAFLGESLTA"
     gene            6630..6863
                     /locus_tag="VKQ27_01715"
     CDS             6630..6863
                     /locus_tag="VKQ27_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87678.1"
                     /translation="MTRGIRGSGAASFAIIGSAKETRLMRKKLAFILAMLGCATTAAC
                     SAGYPPVDPAQRPHTMAAPTSNGDGGGGGGGGY"
     gene            6853..7026
                     /locus_tag="VKQ27_01720"
     CDS             6853..7026
                     /locus_tag="VKQ27_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87679.1"
                     /translation="MDTDVRRVALRTAILWLVLACAACKPPVPATTFTPATGSDGGGD
                     AGGGGGGGAGGSM"
     gene            7103..8680
                     /locus_tag="VKQ27_01725"
     CDS             7103..8680
                     /locus_tag="VKQ27_01725"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HLY87680.1"
                     /translation="MGETLRNNPANTPRVWPFEEAAKVAARLARTGKDHALFETGYGP
                     SGLPHIGTFGEVARTSWVRHAFTELTGLPSRLIAFSDDMDGLRKVPDNVPNRDLLTPH
                     LGKPLTRIPDPFGTHDSFGAHNNARLRSFLDSFGFEYEFASSTEYYLAGRFDDALIHI
                     LECYDEVMAVILPTLGPDRRATYSPILPIHPKTGRVMQVPMESRDPAAGTVVWRDPDT
                     GEAFETPVRGGRCKLQWKADWAMRWYALGVDYEMSGKDLIDSVRLSGRICRTLGSQPP
                     EGFTYELFLDEHAQKISKSKGNGLSVEEWLRYAPPESLSQFMFNAPQRAKRLFFDVIP
                     RAVDEYLANLGKPDAGQPANPVWHIHTGRLPNHGGSPVSFAMLLNLASVVNAETPAIL
                     WGFIRRYSPGASPETMPFLDRLVGHAIAYYRDFVRPEKRYRHPTELERTALNDLAETL
                     KGLDQGTDAEAIQTIVYEVGKRHPFSELRAWFSCLYQVLLGQQEGPRFGGFIALYGID
                     ETIALIEAALARPADAA"
     gene            8684..10723
                     /locus_tag="VKQ27_01730"
     CDS             8684..10723
                     /locus_tag="VKQ27_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLY87681.1"
                     /translation="MLPRPALRIGRTCLRQLPALLGVALLIGAIYVVQKEFRHLRLHD
                     IGVALKDIPAHALAIGFLWTILSYGVLTIYDRLGTIYAGHKVSYSRVAFASFCAYALS
                     HNLGFAAVSGAAVRYRLYAHWGLTPFQIAKTVAFCSLTFALGGLVLGGGVLFLEPRSI
                     PFFGNHLPLFVLYGVGAALWAIVIAYITLSRIVQRIRMFGHELNLPGLRMALAQVLLA
                     TVDVAITASIFYTLLPHAPHLTWPIFLGVYVASYTAGLAANLPGGLGVFDTAILLGLE
                     PYMGAAPVVGAIVVFRLYYYVIPLFLAGFLFTGNEILLRGGGLLRRVEQLAPFQSLAR
                     WSEPDFAVASATGLVALCGMLLLFVSILAPQPDFTWMDPDIAEVANQAGQFIPSLIGA
                     GLIVLAIGLSQRVNLAWTVTIFLLVVGAMFTASQGERLWIAGVLLVVAMLVAPLRRSF
                     YRHARLFTGPLQPSSALSLIALVICLLALAGTRPRVHLLSNNAWWAVIMSRDLPHSVR
                     ATVAVAVALALVAIWRLVRPGKVRWLPWDAAARQRLALLGGTIPPARADGVVLGEAER
                     AGIPFRRCGRVLLGLGDPTGAESDLVSVIWRLRDLAQQEGLDPAFWRAGPALLKVYGD
                     LGLTALPLAPDGTLLPDPGEDEVGARQYLVCVAERDLTTLLPLLPQLANNLPAAA"
     gene            complement(10818..11210)
                     /locus_tag="VKQ27_01735"
     CDS             complement(10818..11210)
                     /locus_tag="VKQ27_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87682.1"
                     /translation="MRNMVRPLATIAVVTGLLGIALQSADAAVQVTDTVGTSGKFLVT
                     GMPVSGPASGFIKIVFGNLTSGTNLELCAGSTGDFSSGTCALSLSSSGGPGFSFLTIV
                     DLAQLNGKVLFVKRAVGTTASKFSLTVE"
     gene            complement(11359..11526)
                     /locus_tag="VKQ27_01740"
     CDS             complement(11359..11526)
                     /locus_tag="VKQ27_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87683.1"
                     /translation="MFPPPFNVDPNWYQNYWFGERPPAKRKALRRSIARVAVLAVLVA
                     GGGFLLSQFHP"
BASE COUNT         2017 a         4346 c         3454 g         2009 t
ORIGIN      
        1 accgcgcaga tgaacggcgc tgacatcatc gtggatttcc tggtccgcca gaacgtgccc
       61 tacctgttcg gcctgtgcgg ccacggcaac gtcggcttcc tcgatgcggc gttcaaggcg
      121 cagaaccgca tccgcaccat ctcggtgcat catgagcagt ccgccggaca catggccgac
      181 gcctatttca aggtccgcca cgaacccgtc gcgacgttca cctcatgcgg tcccggttcc
      241 gccaacctgg tcgtggcgct tgccgccgcg atgatggaca gctcggcgtt ctttgccatc
      301 accggcaacg tgccgaccag tcagttcaat cgccagccct tccaggaaac cggacgctat
      361 ttcgagggcg atttcccttc cgtcatccgt ccctacgtga agcgcagcta ccagcccaca
      421 cgcgtcgaca tggtgccgct cgcagtccgc cagggctggg aactgatgtt gtccggccgc
      481 cccggcccgg tgaatctcga cgtgccgctg aacgtattcg tcgaagaagc cgaaatcacg
      541 ccgccgcttt cctccgcacg tatcgacaat cgtgcatgcg gcaatccgca ggcgttggcg
      601 tcggcgttcg acatgctgct cgccgctgca cgtcccgtca tcatcgccgg ccagggcgtg
      661 ctgcttgccg aagcctccgc cgagctccag caattcgccg aacagttggc gatccccgtc
      721 gcgaccagcc cgaacggcaa aggtgcaatc aatgaacgcc acgccttgtc gctaggttca
      781 atcggccgca acggcaccta cgccgccaac gacgcaacca agaacgccga tgtcatcctg
      841 tcgctcggct tcacgtttga cgatcgcgcc accagcgcct ggctcgatgg ctatacactg
      901 tcggtcccac cgtcgcgcct gatccagatc gacatcgacc ccgccgaact tggccgcaac
      961 tacccggcgg aactacccat actcggctgc gcacgcgcca gcctcgaaca catgaacaag
     1021 cttgcacgcg gcagaaagtc cgccaatcgc gacgcctggc tctcccgcct gcagcgcggc
     1081 cgcgaagtct ggcgtgaata ccaaggcggc ctcgcctcct ccagccagat gccgatgcgg
     1141 cccgaacgcc tgatgcgtgc attgtctaac gcgctgccgg acaacgccat catcgccagc
     1201 gatgtcggcg tacatcacaa ctggatcatc cagttgttcg aggccctccg cccacgtcac
     1261 ctgatccatt cctggggctt tgccgcgatg ggcttcgcca cctccggcat tctcggcgcc
     1321 aagctcgcag cacccgatcg accgtgcgtt gccgtcgtcg gcgacggctc attcctgatg
     1381 actccccacg ccctcgccac agcggtggaa tacgacatcc ccgccgtctg ggtggtctgg
     1441 aacaaccagg gctactgctc gatccgcgac atccagcacg gcatgttcgc cggccgcgag
     1501 ctcgccacca gcttccagca cgacagcaac cgcgagctct acagccccga ctttgccgcg
     1561 ctggcgaaat ccttcggtgt cgcatcccac acggtcacgc acgccgatga ggtcgaggac
     1621 agcatcaaga ccgccatcgc cacgaaccgc ccgtatctga tcgaggtccc tgtcgatcgc
     1681 gagatccgtc ccattggtac cggaagttgg gaactgccgc ccctgccaca accggagccg
     1741 aacttcctca aggccctcgc tgccgccggc ctgtcgctgt agcccctgac cacgatctaa
     1801 cccgcgtccc cctccgtgtc attgcgagga gcgcagcgac gaagcaattc cggcaagaaa
     1861 agggcgtact ttgctcggga ttgcttcgcc cctccgctct ttgcactggc tgttatgccg
     1921 cggcgtccga cgccaccgcc cgcgccactg cctgcggcag ccacacgctc accgtggtgc
     1981 cgatgccttc ctcgctctcg atcgacaact gcccccggtg gcggttgacg atgtgcttta
     2041 cgatagccag ccccaacccc gtgccacccg ccgcgcgcga ccgccccgta tccacgcgat
     2101 agaaccgctc ggtcagccgc ggcagatgtt cgcgcggtat gccggcgccc tggtcggtga
     2161 cagttgtcaa tacacccggc cgccccgaag gcaccgaaat ctcaagccgg accgtgccgc
     2221 cttcgcgccc gtacttcagc gcgttgtcca gcaggttctg cagcacctgg gttacctgat
     2281 ccgcgtcgcc ggtcaccggc ggcacatcgt ccggcatccg caactcaacc ttgaccgacc
     2341 gctctttcaa tcgcggctcg aaaccggcga ccaaccgtgc caccagtccg cgcatatcca
     2401 ccttgcccga tggcgcctga tgctcgctca gttcgatccg cgacaggctc aacagatcgt
     2461 cgatcagccg gttcatccgt gccgcctgct cgctcatgat gccgaggaat cgttgttgcg
     2521 ccggtggatc atccgccgcc ggcccgcgca gcgtgtcgat gaatccaatc aacgacgcca
     2581 gcggcgtgcg cagctcatgg ctgacattgg caacgaaatc cacacgcata cgctccaccg
     2641 cgcgctcgcg ggtgcgatcc gacagcacca cgaccgcccg cccgccatcg gcgatcggcg
     2701 gatccatcgg caccaccgcc gcataaacct cgcgttgcgc cggcaccggc agcgtcactt
     2761 ccgcgatctc cgtcgctcca gacgcgaagg cccgatcgat cgccccacgc aactgcggat
     2821 gccgcagcac cgctcccata tccgtgccga atgcctcgcg cgctgcggaa ttcgcccgcc
     2881 gtacgctgcg atcggcggcc agtacgatca gcggatcggg cagccgctcc accaccgttc
     2941 cgtccgcccg ccgaagctgc tccagcatcg ctgaccgcac ggcggtgcga cgccccaacc
     3001 gatcgagctc acccaccagc cgctgcaatc cgggcagcac cggtatccca ctgctccggc
     3061 tcgttgcctc gtcggcgttc agccggcgca ggctctccgc gacaatgtcc agatcgcgtg
     3121 cccagcgcac agcaaggcca agcgcaccca gcaacgtcac cccgaccgca acagcaaccg
     3181 gccccaccgg agcccatccg agcccagcca gcgcgagcag cccgactacc ggcactccgg
     3241 cgatcgccgc catcgtgccc agaacccgca taacgctagc tgccggtgtc cacgcgccgg
     3301 tgacgcgcgc gaacgccgcg ctcgatggcg gcggcgatca gcggatcgag atccagctcg
     3361 tcacgcacga acgcaagcat ctccagcgtc cgttggcgcg ccaccggact cgcggcgacc
     3421 acttcgcagg caagcgcata cgccgtctcg cgcaacttgg gccccaacgc gccgcacatc
     3481 aggcgcgccg cgtgctgcaa tccgtcctcc tcatccagca gcttcaccgc tgcgtcggtc
     3541 gcaacgcgca gactctccga agtgaaatcc ttgaacgcag gcaacgtctg cacctgaccg
     3601 gccatcacgc cgatctcgcg atcggtcatg ccgccatcag ccgctgccac caggaccatc
     3661 gtcagcacca gcgcctcggg tgcctctagc gcctcgcgcg cacgcatgcg gaactccatg
     3721 gttgttgcca acgaacctga tcgaacggga cggccagttc aagcggtcag cgactcgccg
     3781 aggaacgcgc gcgtttcttt gaccgcttcg gccagtccga tccgccgcac ggtcacgccg
     3841 gcgggaaatg ccgacagcag acgggacggc gtttgccggt ccagcaggac gaacacgcca
     3901 cgatcggtgg cgcgcctgat cagccgtccg aacgcctgcc gcaaccgcag ccgcgcgatg
     3961 cgatcatcgt agcctttggg atcaccatcc gagaggtgta cccggcgttc ccgatgcaga
     4021 atgtccggcc gaggccatgg caccttctcg aacacgacca gccgcaatgc gcgccccggc
     4081 acgtcgacgc cgtcgcgcat cgcatcggtg ccgagcaggc agctctcttc ttcggtgcgg
     4141 aagatatcga ccagcgtggc gttgcccatc gcatcgacgt gctgcgcata gagcgggatg
     4201 ccggcctgct ccaggtcggg agcgatacgt gcatgcaccg cctgcaaccg ccggatggcg
     4261 gtgaacaggc ccagcgcccc gccgcccgac gcccggaaca acgcccgata agccgccgca
     4321 agctgcgcca catcaccggt aattacatcc gtaacgataa acgcgcgggt ctgtgccgcg
     4381 taatcgaaag gactggcgac cgccgcacgg attgccggtg agggcagatg cggcgccccc
     4441 acccgcgcct ccgccgcctc ccacgcaaac tccggattcg catcgccagc gtcgcgcaat
     4501 gtcgccgacg tcaccagcaa tccctgcgcc ggcaatgcca atgtcgcggc gaatggtatc
     4561 gtcgggtcca gccagtggcg atgcaggccg acatccaggt cgcgttcgcc gtcgacccgg
     4621 tccagtcgca ggaacatgat gtggttcggc cggttacccg gctccgcctg cgcgtcgttg
     4681 attgcccgca gcatgttctg ccaggcagac agcgggatca acaggcgccg gttcagggag
     4741 cgtccggccg cctcgatccg gttgcgggtc gctgcgtcca tgtcctcggc ttcctcgtcc
     4801 agccgcgcga gcagccgttc gcgcagcgtc gcaatcggtc cggcgacgcg cgccagggct
     4861 cgcgccagcc gttcggccgc cgaaccgaca tccgggcctg gcggaaacac gtcgcattcc
     4921 agcgacccgc gtgccatttc gtccccggcc gtgcgcgcca gtacctgtcg tcggatcagg
     4981 cgcagaaaat cctccgtcgg attcggccgg ccgctctcga cgccgatgtc cggacgctcg
     5041 ctggccagtc gcgaatgcca gccagggcca gggagggcac gcgccgcctg caacgccgcc
     5101 tccagcggtg cctccagcgt cggccgggcc gccaccagtt catcgatccg ccgcttcagc
     5161 ccccgtgcgc gcgatcgtcc cccttccgcg ccgagcagcc aacgccgcat ctcggcagtt
     5221 tccatacccg acagcgcggc cgaaaacgca ccatccgccg catcgaatac atgatggcct
     5281 tcatcgaaca cgtagcggct gggcaccagg ttgtcgtcca gcccgcccca cgccgcctgc
     5341 gtcatgacca gcgcatggtt cgccacgaca agctgcgcac tacgtgcccg ccggatcgta
     5401 tgctccacga aacatcgctt ccagtgtgga cacgctgcgt ggatacactc gccgcgccga
     5461 tcggccaggc cgtatatcgt cgcgccgcca aacagctcag ccagccagcc cggcagatcg
     5521 ccgccctgga tgtcaccgtc gttggtgacg agcgcccagc gcgacatcag ccccatcgcc
     5581 acaacaatgg cgggcgcggc agcgctcacc gcgtcttcca tattcagcag gcagaggtag
     5641 ttctcccgcc ccttgcgcac caccacgcgc ttgcggcgct cgtttgggtc agggaacagg
     5701 tgcgccagct ccgactcgat ctggcgctgc aggtgccggg tgaacgtgct gatccacacg
     5761 ccgccgtggt tcttctccgc ccacaggctg gccggtgcca gataccccag cgtcttgccc
     5821 gtcccggtgc ccgcttccgc cagcaccaga tgcggatcac cccgtgtctc gcgtggcgcg
     5881 aaggccgccg ccgccgctcc ggcataatcg gcctgcccgg gccgctgttc ggcgtgcggt
     5941 ccaagcatcg ccgccagccg cgaacgggcc tcgccttcac tgaccggatg cgatgccggc
     6001 ggcggcatcg gcgccacgtc ctcccattcc ggcagacgct tccacacccg cagtgcttcg
     6061 cccgaaggag ccgcgtccgg ctgccccaac gcgtcggcaa cgaatggcgc ccagctccag
     6121 ccaacctgcc ccatgcgtgc cgccagcccc gcggcatcgc ggttcagcgc gatgtcgcgt
     6181 ccttgcgcga gacgttgcag caatgccgcc gtgagctccg gcagcagcgc cgcttctgct
     6241 tccagcccgc cacgcggagg ctcgaaatcc agcatcagcg caagcccgcg cggcgttggt
     6301 gcggctggcc gtgctggcag cacgaatgcg aacaattcca gcagatcgaa cgccggcatg
     6361 ccgtgcagac caagccggcg aaacgtggcc ggtgcgtgaa ccagcaacgg cggagccatc
     6421 ccgcgcagcc gcgctgccgc ctctgacgcc ggcaacagca gcagttcacc atcggcagta
     6481 aagatcgcag cacggccgtg gccggctacg accgacggtg catcgggcag cgaaatgcgc
     6541 gccatgttcc tgcaaaatag gtcaccagcg atgcgtagag ccggacgcag ccgcaggcaa
     6601 accaccattt gccaacggcg cacgaatccg tgacccgcgg aattcgcggt tcaggcgccg
     6661 cgtccttcgc catcatcggc agtgcgaagg agacgcggct catgcggaag aagctcgcat
     6721 tcatcctcgc catgctgggc tgcgcgacaa cagcggcctg ctcggcaggc tatccacccg
     6781 ttgacccggc acagcgtccg cacaccatgg cggcgccaac ctccaacggt gatggtggtg
     6841 gaggcggtgg tggtggatac tgacgtcagg cgcgtcgcgc tccgcaccgc aatcctgtgg
     6901 ctggtcctcg cctgtgccgc ctgcaagcca cctgttcctg caaccacgtt caccccggcc
     6961 accggctccg atggcggcgg tgatgccggg ggcgggggcg ggggcggcgc gggcggaagt
     7021 atgtgacagc cgcggattaa acctccttta tcgtgccgca ccgtgattga ccccgcgcgc
     7081 cacggtacct agcttccgcc gaatgggcga aacacttcgc aataaccccg caaacacacc
     7141 tcgcgtgtgg ccattcgagg aagcggccaa ggtcgccgcg cgtcttgcca ggacgggcaa
     7201 ggatcatgcc ctgttcgaga ccggctacgg cccctcgggc ctgccgcata tcggcacctt
     7261 cggcgaagtc gcgcgtacct cctgggttcg ccatgccttc accgaactca ccggcctccc
     7321 ttcacgcctg atcgcgttca gcgacgacat ggacggcctg cgcaaggtgc cggacaacgt
     7381 gcccaaccgc gacctgctca cgccgcatct cggcaagccg ctgacccgca tccccgatcc
     7441 cttcggcacc cacgacagtt tcggcgccca caacaacgcc cgcctgcgtt cattcctcga
     7501 ttcgttcgga ttcgagtacg aattcgcctc gtcaaccgag tactacctcg ccggccggtt
     7561 cgacgacgca ctgatccaca tcctcgaatg ctacgacgag gtgatggctg tcatcctgcc
     7621 taccctcggc ccggaccgcc gcgcgacgta ctcgccaatc ctcccaattc atccaaagac
     7681 cggccgcgtc atgcaggtgc cgatggaaag ccgcgacccg gccgccggaa ccgtcgtctg
     7741 gcgcgatccc gacacgggcg aggcgttcga aaccccggtg cggggagggc gttgcaagct
     7801 ccagtggaag gccgactggg cgatgcggtg gtatgcactc ggcgtcgatt acgaaatgtc
     7861 gggcaaggac ctgatcgatt ccgtgcgtct gtcaggcagg atctgccgca cgcttggcag
     7921 ccagccgccc gaagggttca cctatgaact ctttcttgac gagcatgcgc agaagatcag
     7981 caaatcgaag ggcaacggcc tctcggtcga ggagtggctg cggtacgccc cgcctgaaag
     8041 tctgtcgcag ttcatgttca acgccccaca acgcgcgaag cgcctgttct tcgacgtgat
     8101 cccgcgtgcg gtggacgagt acctggcgaa ccttggcaag cccgacgcgg gccagcccgc
     8161 caatcccgtc tggcacatcc acaccggccg cctgcccaac cacggcggca gccccgtttc
     8221 gttcgccatg ctgctgaacc tggcatctgt ggtgaatgcg gaaacccccg ccatcctatg
     8281 gggcttcatc cgccgctaca gccctggcgc gtctcccgag acgatgccct tcctcgacag
     8341 gctggtcggt cacgccatcg cctactaccg cgacttcgtc cgcccggaaa agcgctaccg
     8401 tcatccgacc gagctcgaac gaacggcact gaacgacctc gccgaaacgc tgaagggcct
     8461 cgaccagggc actgatgccg aggccatcca gaccatcgtc tacgaagttg gcaagcggca
     8521 ccccttctcc gaactccgtg cctggttcag ctgtctctat caggtgctgc tcggccagca
     8581 ggaaggcccg cgcttcggcg gcttcatcgc tctgtacggc atcgatgaaa ccatcgcgct
     8641 gatcgaggcc gcgttggcac gtccggccga cgctgcctga tccatgctgc cgcggcctgc
     8701 cctgcgcatc gggcgtacat gtctgaggca gttgcctgcg ctgctcggcg tcgctcttct
     8761 catcggcgcc atctacgtcg tgcagaagga gttccgccac cttcgactgc acgatatcgg
     8821 ggtcgcgctg aaggatatcc ccgcgcacgc actcgccatc ggcttcctgt ggaccattct
     8881 gtcctacggc gtgctcacca tctacgatcg cctgggcacg atctacgccg gccacaaggt
     8941 gtcatatagc cgggtcgcct tcgcctcgtt ctgtgcctac gcgctgtcgc ataacctggg
     9001 cttcgccgcg gtgtccggcg ccgcggtgcg ttaccggctc tatgcccatt ggggccttac
     9061 gccattccag atcgccaaga ctgtcgcgtt ctgcagcctg accttcgcac ttggcggctt
     9121 ggtgctgggc ggcggcgtgc tcttcctcga gccccgctcg atcccgttct tcggcaacca
     9181 cctgccattg ttcgtgctgt acggcgtcgg cgcagccttg tgggccatcg tcatcgccta
     9241 catcacgctg tcgcgcatcg tgcagcggat caggatgttc ggccacgaac tgaacctgcc
     9301 tggcctccgc atggcgctcg cacaggttct gctggccacg gtcgacgtcg cgatcaccgc
     9361 atcgatcttc tacacgctgc tgccgcacgc gccgcatctc acctggccga tcttcctcgg
     9421 cgtctacgtc gcctcctaca cggcaggcct cgccgccaac ctgccgggcg ggcttggcgt
     9481 gttcgacacg gcgatcctgc ttggcctcga accatacatg ggcgccgcac ccgtcgtcgg
     9541 cgcgatcgtg gtgttccgcc tctactacta cgtcattccg ctgttcctcg ccggcttcct
     9601 gttcaccggc aacgagatcc tgctgcgcgg cggcggcctg ctgcggcgag tcgaacagct
     9661 cgcccctttc cagtcgctgg cgcgctggag cgaacctgat ttcgccgtcg ccagcgccac
     9721 aggcctcgtc gcgctgtgcg gcatgttgct gctgttcgtc agcatcctgg caccacagcc
     9781 cgacttcacc tggatggatc ccgacatcgc cgaggtcgcc aaccaggccg gccagttcat
     9841 tccctccctc atcggcgccg gcctgatcgt gctggccata ggcctgtccc aacgcgtcaa
     9901 cctcgcctgg accgtcacca tcttcctgct cgtggtgggc gcgatgttca ccgcctcgca
     9961 gggcgagcgc ctgtggatcg ccggcgtgct gctcgtggtg gcgatgctgg tggcgccgct
    10021 tcgccgcagc ttctatcgcc acgccaggct gttcaccgga ccgctccagc catcctccgc
    10081 gctttcgctg atcgcgctgg tgatctgcct gctggcgctc gccggcaccc ggccgcgggt
    10141 acacctgctg tccaacaacg cgtggtgggc cgtgatcatg tcgcgcgacc tgccgcattc
    10201 cgtgcgtgcc acagtcgcgg tggccgtggc gcttgccctg gtcgcgatct ggcgtctggt
    10261 gcgccccggc aaggtacgct ggctgccctg ggacgccgcc gcgcgccagc gcctggcgct
    10321 gctcggcggc acgatcccgc cagcccgagc cgatggcgtg gtactcggcg aggccgagcg
    10381 agccggcatt cccttccgcc gctgtggccg tgtgctgctc ggcctgggag accccaccgg
    10441 cgccgagagc gatctcgtct ccgtcatctg gcgcctgcgc gacctcgcgc agcaagaagg
    10501 gcttgatccc gcgttctggc gcgccggccc cgcgctattg aaggtctacg gcgatcttgg
    10561 cctcaccgcg ttgcccctcg cgccagatgg caccctattg cccgacccag gagaggacga
    10621 agttggcgcc cgccaatatc tggtctgtgt cgccgaacgc gatctgacca cgctgctgcc
    10681 gctgctgccg caactggcca acaaccttcc cgccgcagcc taagagcagg ccgcgaggca
    10741 aatggaaccc ccaagcacgc ggattaagca aagggccgcg tgccaccccg caggtcttgg
    10801 ccggccttga gccggcatca ttcgaccgtc agggagaact tggacgctgt cgttccgacg
    10861 gcacgtttaa cgaacaacac tttcccgttc agctgcgcaa gatcgacgat ggttagaaaa
    10921 ctgaacccag ggccgcctga gctcgaaagg gagagcgcac aggtcccgga gctgaagtcg
    10981 ccggttgacc cggcgcatag ctccagattg gtcccgctcg tcagattacc gaacacaatc
    11041 ttgatgaagc cgctggcggg gccgctcaca ggcatcccgg tgacaagaaa cttgccactg
    11101 gtgccaactg tgtctgtcac ctggacagcc gcatcagcgc tttgcagggc aataccgagc
    11161 agtccggtga caacggcaat tgtggcgagt ggtctgacca tattccgcat tgaaacgttc
    11221 ccttcgcgct acccacatgc accggctcgt cgctggtccc ccaccaccgg ttcgcatgct
    11281 tcgagcgtaa ccacaaagcc taacgggacg gtgtgagacg cttcacaacg tacatggcat
    11341 tttgtcacca atgctgatct agggatggaa ttgactgagc aggaaaccac cacctgccac
    11401 gagcacagca agtaccgcaa cgcgggcgat gctgcggcgg agcgccttgc gtttcgcagg
    11461 cggccgttcg ccgaaccaat agttttgata ccaattggga tcgacattga agggcggcgg
    11521 gaacatcagg actacctctg ctggatgata tcgacgacag ccgtcgattt ccttccgggt
    11581 tcatctcgca gatgccgccc agacaatatg aagtgtttca gtcctcagcc tcatcaggtt
    11641 ggcgctacac acaacctatg ccctcgatca gaccgcgctc gaactcctgg tcgaggttct
    11701 aggcttgcct ccccgagacg atacagatcg cgaaggcctt agtgcaccga gcgttcaatt
    11761 ggttcgagcg cgtcgcgcta gtagactgac tactcagcaa ggtgaggtcg agattgccat
    11821 ccgggc
//
LOCUS       DATGEM010000061         5821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_96015, whole genome shotgun sequence.
ACCESSION   DATGEM010000061 DATGEM010000000
VERSION     DATGEM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96015"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VKQ27_01745"
     CDS             <1..319
                     /locus_tag="VKQ27_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87684.1"
                     /translation="TVDAQENPLTNMVNFDVQAHHKHVSLTGHLLGIAPLLVNRARYD
                     ALPAESQAALNAAARDSEATQRALAITDDAECLRTLADAGVAIIPSHEIDLAAFRHVA
                     TGT"
     gene            462..1703
                     /locus_tag="VKQ27_01750"
     CDS             462..1703
                     /locus_tag="VKQ27_01750"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HLY87685.1"
                     /translation="MRLVVLDDDAATTEFMATVARRRGWSVSTATHPNAFRELIAAAP
                     PDAIVLDLQLGASDGIEQLRFLHARNYRGNIVLMSGFDARVLASAQEIGDSLGLTIAA
                     VIEKPARIARIEGVLAAIEKAPAKPQAATMPEPAGPRPISPDDIDEATAAGRMTLQLQ
                     PIVSAGGAVDRAEALIRWSDPSRGPVLPDDFVAVAESDGAVMDRLTMWVAETAAQHYR
                     RLARLGEPVQICINISGCNLRDADFPDRMAALRERMSVPWGGLAMEITESVAMDDLDA
                     TASVLARLRLKGFAVALDDFGTGHSSLTTLRRMPFSTIKIDKSFVQDLLTSNDSLNIV
                     RSVIQLAHDMRLSSVAEGVETAEVASRLIELGIDAMQGYYFSKPLPFDAFAEWLQARR
                     AATSPSPLPTSGGGSGTGCLS"
     gene            complement(1714..2991)
                     /locus_tag="VKQ27_01755"
     CDS             complement(1714..2991)
                     /locus_tag="VKQ27_01755"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY87686.1"
                     /translation="MLSREENELVTRVGQGTPMGNTMRRYWIPAALSREVAEPDGPPV
                     RVRLLGEDLVAFRDSEGKIGLIDELCPHRRASLFFGRNEECGLRCVYHGWKFDISGAC
                     VDMMNEPPENDFKHKVRLTAYPTCELGGIVWAYLGPVEHMPPLPKFAWTQAPEANRHV
                     TKVIQECNWLQGLEGGIDTSHAPIMHRLLSETSKRGGFKPSNPFVRGKAPNLVVDITD
                     YGYQYAGIRPLGDDEMHIRTYQFVLPFHQIRPSKAESGLPLDAGHIWVPIDDHTTAVY
                     NWGYCPDGPLHEEDRLERGLGNGPMHVDPVTFRSKANRENNYMLDRLVQKTESYTGID
                     GINVQDRAIQESMGRIVDRSKEHLGPADKAIIQARKLLRQAVKTVEAGGSPDGTSASY
                     YLLRAHEAVLPRSADWRAEMTPEMKSEAILQTV"
     gene            complement(3018..4001)
                     /locus_tag="VKQ27_01760"
     CDS             complement(3018..4001)
                     /locus_tag="VKQ27_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87687.1"
                     /translation="MSIQGLGYLGIGTDKLDDWADFATNWLGMQAVDCGAGVRAFRMD
                     DRKQRLVVDRALPEGQRYFGWEVADAAALDALAARVEGAGVAVTREPAALADQRFVSA
                     LISFADPAGNRLEAFHGPQVADAAFKPGRSISGFRTGPLGMGHAVLTVTDVDAALKFY
                     LDVLGFRISDFIRTPITAYFLHTNARHHSVALFHAPHVGMHHLMVELYSFDDVGQGYD
                     IALGEREKIVATLGRHPNDLITSFYMRTPSNILVEYGWGGLEVNDATWQPQEMKSVVS
                     FWGHHGLFDGMGPNGPPPMPPLEVTRAPMQVIEGNYHRMSGVCPWWDAMKR"
     gene            complement(4032..5297)
                     /locus_tag="VKQ27_01765"
     CDS             complement(4032..5297)
                     /locus_tag="VKQ27_01765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLY87688.1"
                     /translation="MPDVDVVVIGAGLAGLGAATALRAAGRSAVVLEATERIGGRAWT
                     TLPPELGGVWFDMGAVWLHNAETNPLVPLARAAGETLLRSDELRVERVFVGARQATAE
                     EVADLGGAWQRFEDRAAELLREGGDVPLAAVARSMPDDPWAVTVETWEGPIICVASAD
                     EFSTRDWLRNVLTGSNLVPDGGIGAFVARRLGVRLDIRLSTPVERVRWGGDGVAVETA
                     RGTVTGRSCIVTVSTGVLGAGAIAFEPALPVATLAAIEALPMGLAMKVALRATGPDRL
                     GLPLHCSLERQVARSGDPTMGFQCWPYKRDYIQGWVGGPAAWDLAREGEAAAIDFALG
                     QLRAVFGSRVDRVFAGGTHLVTRWEADPWVRGAYSFVRTGDADARVALAQPVGDGRLL
                     FAGEACHDGMAGTLAGAWISGQDAARLAA"
     gene            5336..>5821
                     /locus_tag="VKQ27_01770"
     CDS             5336..>5821
                     /locus_tag="VKQ27_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87689.1"
                     /translation="MPDSIQPGADNLVIDMVRRFATERLAPGAAAREKAGCIEPEIIR
                     ELGELGIFGATSPAQWDGSELDPVTYAQVLEEVAAGDGSVSTMVSVHNSPTCIIFDQY
                     GTDAQKDRWLRPLATGTAVGSFALTEPQAGSDASNLKTRAVKKGDRYVINGAKQFITS
                     AN"
BASE COUNT         1038 a         2226 c         1617 g          940 t
ORIGIN      
        1 gaccgtggat gcccaggaaa atccgctcac caacatggtg aacttcgacg tccaggcgca
       61 ccacaagcat gtcagcctga ccggccatct gctcggcatc gcgccgctgc tggtgaatcg
      121 ggcgcgctac gacgccctgc cggccgagtc acaagccgcg ctgaatgccg ccgcccgcga
      181 cagcgaagca acccagcgcg ccctggccat caccgacgac gccgaatgcc ttcgcaccct
      241 tgccgatgcc ggcgtggcca tcatcccctc tcacgagatc gacctcgccg ccttccgtca
      301 cgtggcaacg gggacctgag gttggtcgct tcgcaccact tttgcataaa aaatgcgtcc
      361 aggtcgccta atttgcccgc cccctgttta cttccccctt tacctggctt catgtggtgt
      421 gagacgaatt gcagaacctg agcggtatcc ggttgccgaa tatgcgcctg gtcgtgttgg
      481 atgatgacgc tgcaactacc gagttcatgg cgacggtcgc gcgccgccgt ggctggagtg
      541 tcagtacggc aacgcatccg aatgcattcc gagaactgat cgccgcagcg ccgcccgacg
      601 cgatcgtgct cgacctgcaa ctcggtgcaa gcgacggcat cgagcagttg cgtttccttc
      661 acgcccgcaa ctaccgcggt aacattgtgc tgatgagcgg gttcgacgcc cgtgtgctcg
      721 cttctgcgca ggagatcggc gattcactcg gcctgaccat tgccgcggtg atcgaaaaac
      781 cggcgcggat cgcacgcatc gagggagttc tggcggcgat cgaaaaggcg ccggcaaaac
      841 cccaggccgc cacgatgccg gaacccgccg ggccacgtcc gatctccccg gatgatatcg
      901 acgaggcaac cgcggccggc cgaatgacgc tgcaattaca accgatagtc agtgccggcg
      961 gcgcggtcga tcgcgccgag gccctgatcc ggtggtcgga cccgtcgcgt ggccccgtac
     1021 tgcctgacga cttcgtcgca gtcgcggaaa gcgacggcgc ggtgatggat cggctcacca
     1081 tgtgggtcgc agagactgcg gcccagcact accgccggct ggcgaggctc ggcgagccgg
     1141 tgcagatctg catcaatatt tccggctgca acctgcggga cgccgatttc cccgatcgca
     1201 tggcggcact gcgcgaacgc atgtccgtac cctggggcgg cctggccatg gagatcaccg
     1261 aaagcgtcgc gatggacgac ctcgatgcga cggcctccgt gctggcgcgg ctccgcctta
     1321 aaggctttgc cgtggcgctc gacgacttcg gtaccggcca ttcctcgctg accacgctgc
     1381 gtcgcatgcc gttctcaacg atcaagatcg acaagtcgtt cgtgcaagac ctgctgacct
     1441 cgaatgattc actcaacatt gtccgctcag tgatccagct tgcgcacgac atgcgccttt
     1501 ccagcgttgc cgagggcgtc gaaaccgccg aagtcgccag tcgcctgatc gagctcggga
     1561 tcgacgcgat gcaaggctac tacttcagca agccgctgcc cttcgacgcc ttcgcggaat
     1621 ggctacaggc tcgtcgcgcc gcaacttccc cgtcccctct cccaaccagc gggggaggat
     1681 cggggacggg gtgcctcagc tagagaacga cgcctaaacc gtctgcagga tcgcctctga
     1741 cttcatctcc ggcgtcatct cagcccgcca gtccgcactc cgcggcagca ccgcctcatg
     1801 cgcacgcagc agatagtagc tggcgctcgt cccatccggc gaaccacccg cttccaccgt
     1861 cttcaccgcc tgacgcagca gcttgcgcgc ctggatgatc gccttgtccg ccggccccag
     1921 atgctccttc gagcgatcga cgatgcgtcc catgctctcc tggatcgccc ggtcctgcac
     1981 gttgatccca tctataccgg tgtagctctc cgtcttctgc accagccgat cgagcatgta
     2041 gttgttctca cggttcgcct tcgaccggaa ggtcaccgga tcgacatgca ttggcccgtt
     2101 gccaagcccg cgctccagcc ggtcttcctc atgcagaggc ccatccgggc agtatcccca
     2161 gttgtatacc gccgtggtgt ggtcatcgat cggcacccag atatgccccg catccagcgg
     2221 cagcccgctc tccgccttcg atggccggat ctggtggaac ggcagcacga actgatacgt
     2281 ccgtatatgc atctcgtcat cgccgagcgg ccgtatcccc gcgtactgat agccatagtc
     2341 cgtgatatcc accaccagat tcggcgcctt cccgcgcacg aacggattgg acggcttgaa
     2401 cccgccccgc ttcgacgtct cgctcagcag ccggtgcatg atcggcgcgt gcgatgtgtc
     2461 gatgccgcct tccagcccct gcagccagtt gcactcctgg atcaccttcg tcacgtgccg
     2521 gttcgcctcc ggcgcctgcg tccaggcgaa cttgggcagc ggcggcatgt gctccaccgg
     2581 cccaagatac gcccacacga tcccacccag ctcgcaggtc ggatacgccg tcagccgcac
     2641 cttgtgcttg aaatcgtttt ccggcggctc gttcatcata tcgacgcacg cgccggaaat
     2701 gtcgaacttc cagccatgat acacgcagcg cagcccgcac tcttcattcc ggccaaagaa
     2761 cagcgacgcc cgccggtgcg ggcaaagttc gtcgatcagc ccgatcttcc cctcgctgtc
     2821 gcgaaacgcc accaggtcct cgcccagcag ccgcacgcgt accggcggcc catccggttc
     2881 ggcaacctca cgggacagcg cggccggaat ccaataccga cgcatcgtgt tgcccatcgg
     2941 cgtgccctgg ccgacgcggg tgacgagttc gttctcttcg cgtgacagca tgttcgtccc
     3001 tccgtgcgtt gtctcgacta tcgcttcatc gcatcccacc acgggcaaac gccgctcatc
     3061 cggtggtaat tgccctcgat cacctgcatc ggcgcgcgcg tcacctccag gggcggcatc
     3121 ggcggcggcc cattcggccc catgccatcg aacaatccgt gatgtcccca gaagctgaca
     3181 acactcttca tctcctgagg ctgccacgtc gcatcgttaa cctccagccc accccagcca
     3241 tactccacca ggatgttcga cggtgtgcgc atgtagaacg acgtgatcag atcattcggg
     3301 tgccgtccca gcgtcgccac gatcttctcg cgctcaccga gcgcaatgtc atagccctgc
     3361 ccaacgtcat cgaacgaata cagctccacc atcagatgat gcatcccgac atgcggcgca
     3421 tggaacaacg ccacactatg atgccgggca ttggtgtgca ggaaatacgc cgtgatcggc
     3481 gtgcgaataa aatcgctgat cctgaatccc agcacatcaa gatagaactt cagagccgca
     3541 tcgacatcgg tgacggtcag caccgcgtgc cccattccca gcggcccggt ccggaatccc
     3601 gaaatcgacc gccccggctt gaacgccgca tcggccacct gaggcccgtg aaatgcctcc
     3661 agccgattgc ccgccggatc ggcgaatgag atcagcgcgc tcacgaatct ctgatccgcc
     3721 agcgccgccg gctcgcgcgt caccgcgacg ccggcaccct caacccgcgc cgccagcgca
     3781 tcgagcgccg cggcatcggc cacttcccac ccaaagtacc gctgcccctc cggcagggcg
     3841 cgatccacca ccagccgctg cttccggtca tccatgcgga acgcgcgcac gcccgcgcca
     3901 caatccaccg cctgcatgcc gagccagttg gtcgcgaaat ccgcccagtc gtccagctta
     3961 tcggtgccga tgccgagata gccgagccct tgtatggaca ttgtccaatc cccctgtttg
     4021 ggggcaacat atcacgcggc caaacgcgcc gcatcctgtc cgctgatcca cgcaccagcc
     4081 agcgtgcccg ccatcccgtc atggcaagcc tcgccggcga acagcagccg cccatccccc
     4141 accggttgcg ccagcgccac ccgtgcgtcc gcatcgcccg tccgtacaaa cgaataggca
     4201 ccgcgcaccc agggatcagc ttcccaccgc gtcaccaggt gcgtcccgcc cgcgaacacc
     4261 cgatccaccc gcgacccaaa cacggcccgc aactgcccca gcgcgaagtc gattgccgcg
     4321 gcctcgccct cgcgcgccag gtcccacgcc gccgggccgc cgacccagcc ctggatgtag
     4381 tcacgtttgt agggccagca ctggaacccc atcgtcggat cgccgctgcg cgcaacctgc
     4441 cgctccagcg agcaatgcag gggcagcccc agccgatcgg gacccgtcgc ccgcagcgcc
     4501 accttcatcg ccagccccat cggcagcgcc tcgatcgccg ccaatgtcgc caccggcagc
     4561 gccggctcga acgcgatcgc cccagccccc agcacgcccg tcgacacggt cacgatgcac
     4621 gaccgccccg tcaccgttcc ccgcgccgtc tccaccgcaa caccatcacc gccccagcgc
     4681 acacgctcca ccggtgtaga caggcgtata tccagtcgca cccccagccg ccgcgcgacg
     4741 aacgcgccga tcccgccatc cggcaccagg ttgctgccgg tcagcacatt ccgcagccag
     4801 tcgcgcgtgc tgaattcatc ggccgaggcc acgcagatga tcggcccctc ccacgtctcc
     4861 accgtgaccg cccaaggatc atccggcatc gaccgcgcca ccgccgccag cggcacgtca
     4921 ccgccctcgc gcagcagctc cgccgcccgg tcctcgaacc gctgccacgc gccacccagg
     4981 tccgccactt cctctgccgt cgcctgccgc gcgccgacga acacccgctc cacccgcagc
     5041 tcgtcggatc gcagcagcgt ctcccccgcc gcgcgcgcca acggcaccag cgggttggtc
     5101 tccgcgttgt gcagccacac cgcgcccata tcgaaccaca caccgcccaa ttccggcggg
     5161 agcgtggtcc aggcgcggcc gccgatccgc tccgtcgctt ccagcaccac cgcggaccgg
     5221 cctgccgcac gcaaggccgt cgcggcaccc agcccggcca ggccggcgcc aatcaccaca
     5281 acatcgacat cgggcatgcc gcgaacctat cgtgacccat cgaaggaggc cagtcatgcc
     5341 cgacagcatc cagcccggcg ccgataacct cgtgatcgac atggtccgcc gcttcgccac
     5401 cgagcgcctc gcccccggcg ccgccgcccg tgaaaaggcc ggctgcatcg aaccggagat
     5461 catccgcgaa ctgggcgaac tcggcatctt cggcgccacc tcgcccgcgc aatgggacgg
     5521 ctccgagctc gacccggtga cctacgcgca ggtgctggag gaggtcgccg ccggcgacgg
     5581 ctccgtctcc accatggtga gcgtgcacaa ctcgcccacc tgcatcatct tcgaccagta
     5641 cggcaccgac gcgcagaaag accgctggct gcgcccgctc gccaccggca ccgctgtcgg
     5701 ctccttcgca ctgaccgaac cgcaggccgg ctccgacgcg tccaacctga agacgcgcgc
     5761 ggtgaagaag ggcgaccgct acgtgatcaa cggcgccaag cagttcataa cctcggcgaa
     5821 c
//
LOCUS       DATGEM010000062         4955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1464524, whole genome shotgun sequence.
ACCESSION   DATGEM010000062 DATGEM010000000
VERSION     DATGEM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464524"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..795
                     /locus_tag="VKQ27_01775"
     CDS             124..795
                     /locus_tag="VKQ27_01775"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HLY87690.1"
                     /translation="MALADDIGHILAPPHAAGRPFILGGLVAILLGLILAHWLAWLGL
                     IFTLFCTFFFRDPERVPPGRAGTVVAPADGRVLAILPAVPPAELGLGAMPRWRVSTFL
                     SLVNVHVNRIPADGTVTRIAYRHGSFLNASSDKASDENERNAIALRLGDGREIAVVQI
                     AGLVARRIVCDVREGDTVHAGQRFGIIRFGSRADIYLPEGVRPLVAVGQTMIGGETVI
                     AELGG"
     gene            792..1661
                     /locus_tag="VKQ27_01780"
     CDS             792..1661
                     /locus_tag="VKQ27_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HLY87691.1"
                     /translation="MSGAPRPEWPGGPRVSPMKRLRRLRPRPRPRFKGPSFNRIIPNL
                     LTLMGLCAGLTSLRLALEGRFGAAAVAIVVAMAIDGLDGRLARLLNATTRFGAEFDSL
                     TDFLCFGVAPAFTLYLWSLQRAGGFGYTPCLMFAVCMALRLARFNASLDAGPGPAYAY
                     NFFTGVPAPAGAALVLFPLFLGLEARSIGWDWLLAVMQYPLFCALVLAGTAVLLVSTL
                     PVWSFKNFKVPHEYVLPLLLGAGTFAAVLVADPWLALATGDLIYLAMLPFSVRSFRRL
                     RRAAEEMLEAAEE"
     gene            1783..3072
                     /locus_tag="VKQ27_01785"
     CDS             1783..3072
                     /locus_tag="VKQ27_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HLY87692.1"
                     /translation="MSLSLTDRAVTAGREAISGQRRGLRAFLPFAGPAVIASIAYMDP
                     GNFATNIQGGAQFGYLLLWVVVLANVVAMLFQAMSAKLGIVTGQSLAQLCRTHFPRPL
                     VYAMWIGSEIAAMATDLAESLGAAIGLSLLFGLPLLPGLLITFGATYGMLLLQGRGFR
                     PIEMLIAAFIAVIVVSYVIELFVAPPNWRLFAYHAVVPQLTGPGSVTLAVGIIGATVM
                     PHAIYLHSSLTQRRMPARTDAERRRILSFSNREVLVALGVAGLVNMAMLAMAATVFHQ
                     GHAEIASIETAYHTLVPLLGVGAGVVFMASLLASGISSSVVGTMAGQTIMQDFVHFRI
                     PLWVRRLAVMIPATVIVGIGVDSTQALVLSQVVLSLVLPIPMIALLVLSGRRDVMGEF
                     ANRRVTQIGAVVAAALVLTLNLVLLLQTAGIPVPYLG"
     gene            3134..3550
                     /gene="mntR"
                     /locus_tag="VKQ27_01790"
     CDS             3134..3550
                     /gene="mntR"
                     /locus_tag="VKQ27_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HLY87693.1"
                     /translation="MPAEPTQAQRFGKARTAQSSALSEDYVELISDLLANGGEARPTD
                     IARRLGVSHATAIKTIGRLKREGLAVAKPYRGVFLTEAGEKLADRVRARHRVVVDLLL
                     AVGVPAEAAEQDAEGIEHHVSDTSLAAFGRFLRRRG"
     gene            3550..4389
                     /locus_tag="VKQ27_01795"
     CDS             3550..4389
                     /locus_tag="VKQ27_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY87694.1"
                     /translation="MRCTVCETGAAARYRTVDGIAYFRCKACTSLFADPGFEPAPYAG
                     DYWRNEIGPARERCFGPALMRVAETIAYCRLPIQAFIDIGAGTGWLLDALETLLPEVI
                     DRFHAVELYPPPLPYRTGNANYVVGTLADIGHTFDAGVCIEVIEHLAPATLRTLAAQL
                     AAVSRRGALYLFNSGQPDFVEREDPNYLDPHGRGHIASYSLSGAAAIFRPAGFNVIAL
                     PNRTWAFLVEFGTTQPVDGHALIDRLWHPVAENVSMLRAARFGELLRVAGIDTARAYA
                     QLG"
     gene            4404..>4955
                     /locus_tag="VKQ27_01800"
     CDS             4404..>4955
                     /locus_tag="VKQ27_01800"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLY87695.1"
                     /translation="MSVHDHSRWGPTDQIGAGNLLTVEKRLAALASVREGRVYDVSHE
                     ISMGAPFMAPNQTPFLLSIWASWRDSIKRRRALGATNDAGANVERIEMTAHVGTHIDA
                     LGHFSKGDRLYNGLSAAETVTDWGLQRLGIEHAPPMITRGVLLDVSGCDGGQHLNPGR
                     VVTPDELARAAETAGVEIESGDIA"
BASE COUNT          739 a         1646 c         1597 g          973 t
ORIGIN      
        1 gcccggtccc atcgacggtg aagggtccga gcttgaacgg tgcattgaac ggcatggcgg
       61 gcgctccgta cctgccattg atcttgccgc cccaaagtga agcttccata aacctcgaca
      121 cccatggcgc tcgccgacga cattggccac atcctggccc cgccacacgc cgccgggcgg
      181 ccattcatcc tcggcggcct ggtcgcgata ttgcttggcc tgatcctcgc gcactggctt
      241 gcctggctcg gtctgatatt caccctgttc tgcacgttct tcttccgcga ccccgaacga
      301 gtgccgcccg gccgcgccgg caccgtcgtc gccccggcgg atggccgcgt cttggctatc
      361 ctgcccgccg taccgccagc ggaacttggc ctgggcgcca tgccacgctg gcgcgtcagc
      421 acattccttt cactggtcaa cgtgcacgtg aaccggatcc ccgccgacgg gacggtcacc
      481 cgcatcgcct atcggcacgg cagctttctg aatgccagca gcgacaaggc gagcgacgag
      541 aacgagcgga atgcgattgc actccggctc ggagacggcc gggagattgc ggtggtgcag
      601 atcgctggtc tggttgcccg ccgcattgtc tgcgacgtgc gcgaaggcga tacggtccac
      661 gcgggacagc ggttcgggat tatccgcttc ggcagccgcg ccgacattta cctgcccgaa
      721 ggggtgcgtc ccctggttgc tgtcggccag accatgatcg gcggcgagac ggtgatcgca
      781 gagctgggcg gatgagcggc gcgccgcgtc ccgaatggcc gggcggcccg cgcgtttcgc
      841 cgatgaagcg attgcgacgc ctccgtccgc ggcctcggcc gcgcttcaag ggaccttcgt
      901 tcaaccgcat cattcccaac ctgctgacgc tgatggggct ttgcgccggc ctcacctcgt
      961 tgcggctggc tctggaaggc cgcttcggtg cggcggccgt agcaatcgtt gtggcgatgg
     1021 cgatcgatgg actggatggt cggcttgccc gcctgctgaa cgccacgaca cgcttcggcg
     1081 ccgaattcga cagcctgacc gacttcctgt gctttggcgt cgcacccgca ttcacgctct
     1141 acctctggtc tctgcagcgc gccggtgggt tcggctacac accctgcctg atgttcgccg
     1201 tgtgtatggc gctgcggctg gcgcgcttca atgcgtcgct ggatgccgga cctgggcctg
     1261 cctacgccta caacttcttc accggcgtcc ctgccccggc cggcgctgcg ctcgtactgt
     1321 ttccgctgtt ccttggcctt gaagcgcggt cgatcggttg ggactggctg cttgccgtca
     1381 tgcaatatcc gctgttctgc gccctggtgc tggcaggcac cgccgtgctg ttggtttcca
     1441 ccctgccggt atggagtttc aagaacttca aggtgccgca cgagtatgtg ctgccgctgc
     1501 tgctcggcgc cggtacattc gccgcggtgc tggtggcgga tccctggctg gcactggcga
     1561 cgggagacct gatctacctg gccatgctgc cattcagtgt gcgcagcttc cgccgcttgc
     1621 ggcgcgcggc cgaggagatg ctggaagccg cggaagaata ggccccgctg ggagcacaca
     1681 cacgccgtgg ttgactctag cccctccgct cctatctatt gctccaactt gtagccaagg
     1741 ctacattctg tgggagcagg ttcagagtat ggtcacgggt ccatgagcct cagtctgaca
     1801 gatcgagccg tgacggcggg ccgcgaggcg atcagcggcc agcggcgcgg cttacgggcc
     1861 ttcctgccgt tcgcgggccc ggcggtgatc gcctcgatcg cttacatgga tcctggcaat
     1921 tttgccacga acatccaggg cggcgcgcag ttcggctatc ttctgctgtg ggtcgtggtg
     1981 ctggccaacg tggtggcgat gctgttccag gcgatgtcag ccaagctggg catcgtcact
     2041 ggccagagtc tggcgcagct ttgccgcacg cattttcccc gcccgctggt ttacgccatg
     2101 tggatcggct ccgaaatcgc cgccatggcc accgaccttg ctgagtcgct cggcgctgcc
     2161 atcggtctca gcctgctgtt cgggttgccg ctgctgccgg ggctgctgat cacctttggt
     2221 gccacctatg gcatgctgct gctgcagggc cgaggcttcc gcccgatcga gatgctgatc
     2281 gccgcattca tcgcggttat cgttgtctcc tacgtgatcg agctgttcgt tgctccaccg
     2341 aactggcgat tgtttgccta ccacgcggtg gtgccccagc tcaccggtcc cggcagcgtg
     2401 acactcgctg tcggcatcat cggcgcaacc gtgatgccgc atgcgatcta cctgcactcc
     2461 agcctgacac agcgccgcat gccggcgcgc acggacgcgg aacgtcggcg gatcctcagc
     2521 ttctccaacc gcgaggtcct ggtcgcgctg ggcgtggccg ggctggtcaa catggcgatg
     2581 ctggcgatgg ctgccaccgt cttccaccag ggtcatgccg agatcgcctc gatcgagact
     2641 gcctatcaca cactggtgcc gctgcttggc gttggcgctg gcgtggtctt catggcctcg
     2701 ctgctcgcgt ccggcatctc gagttctgtc gtcggcacga tggcgggtca gacgatcatg
     2761 caggacttcg tgcactttcg tattccgctg tgggtgcgcc gccttgccgt tatgatcccg
     2821 gccacggtca tcgttggaat tggtgtggac tcgacccagg cgctggtctt gagccaggtg
     2881 gtgctgagcc tggtgttgcc gataccgatg attgccctgc tggtcctgtc cggccggcgc
     2941 gacgtgatgg gcgaattcgc caaccgccgc gtcactcaga tcggggcagt ggtggccgcg
     3001 gctctcgtgc tgacgctgaa tctggtgctg ctcttgcaga ccgcgggcat cccggtgcca
     3061 tatctcggct gaatcagccg caagagcagg agtcgccttg gccagcccag ggcccatcac
     3121 ccgccggatc gagatgcccg ccgaaccgac ccaggcacag cggtttggca aggcgcgtac
     3181 agcgcaatcc agtgcactca gcgaagacta tgtcgagctg atctcggatc tgcttgccaa
     3241 tggcggcgag gcgcggccca ccgacattgc ccgccgcctc ggcgtctccc atgccacggc
     3301 gatcaagacc atcggccgcc tcaagcgcga agggctggcg gtggcgaagc cctatcgcgg
     3361 cgtattcctg acggaggcgg gcgagaaatt ggccgatcgg gtgcgtgcgc ggcatcgcgt
     3421 cgtggtggac ctgctgttgg ccgtcggtgt gccagcagag gccgcggaac aggacgccga
     3481 gggcatcgag caccatgtct cggataccag cctcgccgcc ttcggccggt tcctgcgccg
     3541 gcgcggctga tgcgctgcac tgtctgcgag acaggcgccg ccgcgcggta ccgcacggtg
     3601 gacggcatcg catatttccg ctgcaaagcc tgcacttccc tgttcgccga tccggggttc
     3661 gaaccagcac cgtacgccgg cgactactgg cgcaatgaga tcggcccagc ccgcgaacgc
     3721 tgcttcggcc cggcactcat gcgcgttgct gaaaccatcg cgtattgccg tctgcctata
     3781 caagccttca tcgacatcgg cgccggcacc ggttggctgc tcgatgcgct ggaaacgttg
     3841 ctgcccgaag tgatcgatcg cttccacgca gtggaactat atccgcctcc cctgccctac
     3901 cgaacgggca atgccaacta cgtcgtcggc accctggccg acatcgggca caccttcgat
     3961 gcaggcgttt gcatcgaggt gatcgagcac ctcgcgcccg caacactccg tacgctggcc
     4021 gcgcagcttg ccgccgtgtc gcggcgtggc gcgctttatc tgttcaactc cggtcagccg
     4081 gacttcgtgg agcgcgagga tcccaattac ctggacccgc acggccgcgg ccacattgcc
     4141 tcgtattcgc tgtccggcgc ggcggcgatc ttccgcccgg ccggcttcaa cgtgatcgct
     4201 ctgcccaacc gcacctgggc tttcctcgtc gagttcggca cgacgcagcc agtcgatggc
     4261 cacgcactga tcgaccggct ttggcacccg gtggcggaga atgtatcgat gttgcgcgcg
     4321 gcgcggttcg gcgaactgct gcgcgtcgcc ggcatcgaca ccgcgcgggc atacgcgcag
     4381 cttggttgaa ctgggaggca acgatgtccg tacatgatca ttcgcgctgg gggcccaccg
     4441 accagatcgg cgccggcaat ctgctgacag tggaaaagcg tctcgccgca ctggcgtcgg
     4501 tgcgcgaggg ccgcgtctac gacgtgagcc acgagatcag catgggcgcg cccttcatgg
     4561 cgccgaacca gacaccattc ctgctgtcga tctgggcgtc ctggcgtgac tcgatcaagc
     4621 gccgtcgtgc gctgggcgcc accaacgatg ccggcgccaa cgtggagcgg atcgaaatga
     4681 ccgcgcatgt cggcacgcat atcgacgcgc ttggccattt ctcgaaaggc gaccggctct
     4741 ataatggtct gtccgccgcc gagacggtga cggattgggg cttgcaacgc ctcggtatcg
     4801 agcatgcgcc gccgatgatc acgcgcggtg tgctgctgga tgtgtcggga tgcgatggtg
     4861 gccaacatct gaatccggga cgagtggtta cgccggatga actggcacgg gcggctgaaa
     4921 ctgccggcgt ggagattgaa tctggcgaca tcgcg
//
LOCUS       DATGEM010000063         4562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_911599, whole genome shotgun sequence.
ACCESSION   DATGEM010000063 DATGEM010000000
VERSION     DATGEM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911599"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VKQ27_01805"
     CDS             complement(<1..546)
                     /locus_tag="VKQ27_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY87696.1"
                     /translation="MPTLAHQLADRIHRLRYEDLSPAAIEWTRTAFIDTIACTLAGIE
                     DDGPRILRRIPGIATAPGPSLIYASRTRTSPLDATLVNGTASHALDYDDVSGVMGGHP
                     SVMLIPPMLALAELIGASGRDLALAYVVGYETECRIARGVHFHHYDKGWHPTATLGIF
                     GTVAAASRMLRFTHDQTATAIG"
     gene            621..1997
                     /locus_tag="VKQ27_01810"
     CDS             621..1997
                     /locus_tag="VKQ27_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY87697.1"
                     /translation="MTIAEALAERVASVQYDALPEDALYWARVAILDTVGCTLAGADE
                     PCARIVARVTAAPGPCLVFGSAQRVAPLDAALINGTAAHAHDFDDCSNTLGGHPSAPI
                     LPALFALAETRAVDGRAFVAAYVAGWEAETRIARGINFYHYEKGWHPTATIGVFGAAA
                     ACSHLLGLAPDATAKALALAASFSSGVKANFGTMTKPLHVGHCSRNGLFAALLASEGF
                     TANPGAMEHRQGFLHVFNGEGNFNAEAMLNDWGSPWDIVQPGVAIKQYPCCGSTHPAV
                     DAMLSLVREHGLTPSMVERVDSWTHPRRLAHTNRPDPQSELDAKFSVQYCLARALLDG
                     RVSLEHFEGDRFRDPAIVALLPRIHAAPHPEMPMTSTEHFGAEVQATLTDGRVLTAKV
                     ARPLGRGPENPLPAELLEAKFLNCAARALPMEAAEALLAVLRRVDTLVDIRQATDAMV
                     VKAALAAD"
     gene            complement(2013..2843)
                     /locus_tag="VKQ27_01815"
     CDS             complement(2013..2843)
                     /locus_tag="VKQ27_01815"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87698.1"
                     /translation="MITQLSDHTGAEVRGVDLSQPIDGALRTRLNQAFVDHSVLVFRD
                     QRLSPHQLLAAVQLFGEVLPQHNTRFALPECPQIHYISNQDTYPDGKRYIPGEGYHTD
                     HSNAAEPPKATVLHAVKLPDQGGDTQFVNMHRAYEDLPSAMRTRIDGLRAAHVYQSRF
                     SERKLMALSDANRARVPDAVLHPLVRTHPETGRKAIYLNPIRIEGIEGMEENEALGLL
                     DQLLSHATQPQYEYRHRWQQGDMVMWDNRCLLHKANGDYDMGQARYLYRIMLKGDVPI
                     "
     gene            2926..3345
                     /locus_tag="VKQ27_01820"
     CDS             2926..3345
                     /locus_tag="VKQ27_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HLY87699.1"
                     /translation="MPSESQTPDFTKFFAELKLPAMPDMEAFLAANRKNIETFSAANR
                     VALEGAQAVAKRQMEMVQSSMSEIADAMKELASADAPQEKAAKQAELLKQAYQRAVAH
                     MKELSDLIRQSNAEALALINKRFEEAMDEVKVLAQKK"
     gene            3668..>4562
                     /locus_tag="VKQ27_01825"
     CDS             3668..>4562
                     /locus_tag="VKQ27_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87700.1"
                     /translation="MNAAASVPLSGLSEQEHRSQLRRAVIASTVGTTIEWYDFLLYGT
                     VTALVFGKLFFPKSNPIVGVLEAFSVFFIGFVGRPIGAFIFGHWGDRIGRKATLIVTL
                     LVTGVATVAVGCVPGYNSIGIWGAVLLTLIRLVQGIGVGGEWGGSVLLAMEWAQTNKN
                     RGFLSAWPQFGAPAGLFLANCAVLFFSWYSGDQFLVWGWRIPFFLSAIMVAVGLWIRL
                     GIMETPVFQRILDEERTERVPVLEVLKRQPKQIILTALLRMPEQAPGYIVGAWIFTYA
                     TGVLGYSRDFVLLGVISQTVLG"
BASE COUNT          775 a         1344 c         1593 g          850 t
ORIGIN      
        1 accgatcgcc gttgcggtct ggtcgtgagt gaagcgcagc atccgcgagg cggcggcaac
       61 cgtgccgaag atgccgaggg tggcggtggg atgccagccc ttgtcgtagt ggtggaagtg
      121 cacgccacgg gcgatgcggc attccgtttc gtagccgacg acataggcga gcgcgagatc
      181 gcgaccggag gcgccgatga gttcggcgag tgccagcatc ggcggaatca gcatcaccga
      241 gggatggccg cccatcacgc cggacacgtc gtcgtaatcc aacgcgtgcg atgcggtgcc
      301 gttcaccaag gtagcgtcga gcgggctggt gcgcgtccgc gacgcgtaga tcagcgatgg
      361 acccggcgcg gtggcgatgc cggggatgcg gcgcaggatg cgcgggccat cgtcctcgat
      421 gccggcgagc gtgcaggcga tggtatcgat gaacgcggtg cgggtccatt cgatcgccgc
      481 cggcgaaagg tcctcgtagc gcaggcgatg aatgcgatcc gcgagctggt gcgcgagtgt
      541 gggcatggtt cgtgtccttg tggacgctct ttcgcaatgc tgtagcatcg ccccagggaa
      601 gtggcgagcg gagggaaccc atgaccatcg cggaagctct ggccgagcgc gttgccagcg
      661 ttcaatacga tgcgttgccg gaagatgcgc tgtactgggc gcgtgtggcg atcctcgaca
      721 cggtgggctg caccctggca ggagccgacg aaccctgcgc gcgtatcgtc gctcgtgtga
      781 cggcggcacc gggtccttgc ctggtgttcg gttcggcaca gcgtgtggcg ccgctggatg
      841 ccgcgctgat caacggcacc gccgcgcacg cgcacgactt cgatgattgc agcaacacgc
      901 tgggtgggca tccctcggca ccgatcctgc cggcgctgtt cgcgctggcc gagacgcggg
      961 cggtggatgg acgcgcgttc gtcgcggcgt atgtggcggg gtgggaagcc gagacgcgga
     1021 tcgcgcgcgg tatcaacttt tatcactacg agaagggctg gcatccgacc gcaacgatcg
     1081 gtgtgttcgg cgcggcggcg gcgtgttcac atctgctggg gctggcgccc gacgcgacgg
     1141 caaaggcgct ggcactggct gcatccttct catccggggt gaaggcgaat ttcggtacga
     1201 tgacgaagcc gctgcatgtg gggcattgct cgcgcaacgg gctgttcgcc gcgctgctgg
     1261 catccgaggg ctttaccgcc aatccgggcg cgatggagca caggcagggc ttcctgcatg
     1321 tgttcaacgg cgagggtaat ttcaacgccg aggcgatgct gaacgattgg ggttcgccgt
     1381 gggacatcgt gcagcctggt gtcgcgatca agcaatatcc gtgctgcggc agcacccatc
     1441 ccgcggtcga cgcgatgctg tcactggtgc gagagcatgg gctgacgccg tcgatggtgg
     1501 aacgcgtcga ttcatggacg catccccgcc gcctggcgca caccaaccgg cccgatccgc
     1561 aaagcgagct ggatgcgaaa ttcagcgtgc agtactgcct ggcgcgtgca ctgctggacg
     1621 ggcgggtgtc gctggagcat ttcgagggcg accgcttccg ggatccggca atcgtcgcgc
     1681 tgctgccacg cattcatgcc gcaccgcatc ctgagatgcc gatgacaagc actgagcatt
     1741 ttggggcgga ggtacaggcc acgttgaccg atggccgggt actgacggcg aaggtggcgc
     1801 ggccgttggg gcgagggccg gagaacccgc tgccggcgga gttgctggag gcgaagttcc
     1861 tcaactgtgc ggcgcgcgca ctgcccatgg aggcggctga ggcgctgctg gcagtgctgc
     1921 ggcgtgtcga tacgctggta gatatacggc aggcaaccga tgcgatggtg gtgaaggcgg
     1981 cgctggcggc ggattagccc gcgatgacaa ggtcagatgg gcacgtcgcc cttcagcatg
     2041 atgcggtaga gatatcgcgc ctgccccatg tcgtagtcgc cgttcgcttt gtgcagcagg
     2101 caacggttgt cccacatcac catgtcgccc tgctgccagc gatgacggta ctcgtattgc
     2161 ggctgcgttg catgcgacag cagctgatcc agcagaccta gcgcttcgtt ctcctccata
     2221 ccctcgatgc cctcgatgcg gatcgggttg aggtagatcg ccttgcgccc ggtttctgga
     2281 tgcgtgcgca ccagtggatg cagcacggcg tccggcacgc gcgcgcgatt ggcgtcgctc
     2341 agcgccatca gtttccgttc gctgaagcgg ctctggtaga cgtgcgccgc acgcagtcca
     2401 tcgatccgcg tgcgcatggc agacggcaga tcctcgtatg ccctgtgcat attgacgaac
     2461 tgcgtgtcgc cgccctggtc cggcagtttt accgcatgca gcacagtcgc cttcggcggt
     2521 tcggcggcgt tggaatggtc ggtgtgataa ccctcgccgg gaatgtagcg cttgccgtcc
     2581 ggataggtgt cctggttcga gatgtagtgg atctgcgggc actccggcag cgcgaagcgt
     2641 gtgttgtgct gcgggagcac ctcgccgaac aactgcacgg cagccagcag ctgatgcggc
     2701 gacagacgtt ggtcgcggaa caccagcacc gaatggtcca cgaaggcctg gttcagccgt
     2761 gtgcgcagcg cgccatcgat cggctgggac aagtcgacgc cgcgcacctc ggcgccggta
     2821 tgatcagaga gttgcgtaat catggaactg agattgcgcc ccggttcgcg cgcgtgcaac
     2881 gtgtgagggc gcagcggaca gccgcatcaa ctgaaggagt tggccatgcc aagcgaatcg
     2941 caaacgcccg acttcaccaa gttcttcgcc gagttgaagt tgcccgcgat gcccgacatg
     3001 gaggctttcc tggcggctaa ccgcaagaac atcgagacat tttctgccgc caatcgcgtc
     3061 gcgttggagg gtgctcaggc ggtggccaag cggcagatgg agatggtgca gagcagcatg
     3121 tcggagatag ccgacgcgat gaaggaactc gcatcggccg atgcgccgca ggagaaggcg
     3181 gcgaaacagg cggaactgct gaagcaggcg taccaacggg ccgtggcgca tatgaaggag
     3241 ctgagcgacc tgatccggca atccaatgcc gaggcgctgg ctctgatcaa caagcgcttc
     3301 gaggaggcga tggacgaggt aaaggtgctg gcgcagaaaa aatagcgcgc gccttctgcc
     3361 gcctctcttc gaccgaggcg tggcggccgt tcaagcgtcc ataggccttg ctgcgggccc
     3421 cggcaagtaa tcacggtacg attgccgatc tttcaaaaat caaatgggaa cgtggtcgca
     3481 ttttcgtgcg gtcgggccgc ggttaatatt gcacgacttc cttgccaaac cggcacgcta
     3541 cgatgcaggg tctacgcgcg ctggcgtcgt tctcccgcaa cgccacgacg actcgctgcg
     3601 gccatcggcg ccggagccgg aatggaacgg gcacatcaag tgacccgcga agcagggaga
     3661 atttgccatg aatgcagccg cgtccgtgcc gttgagcgga ctttccgaac aggaacacag
     3721 gtcacagctt cgtcgtgcgg tcattgccag cactgtcgga accacgatcg agtggtacga
     3781 cttcctgctg tatggcacgg tcaccgcgct ggtcttcggc aagctgttct ttccgaaatc
     3841 caatcccatc gttggcgtgc tggaagcatt cagcgtgttc ttcatcggct tcgtgggccg
     3901 gccgatcggt gcgttcatct ttggccactg gggcgaccgg atcggacgca aggcgacgct
     3961 gatcgtgacg ctgctggtca ccggcgttgc caccgtcgcg gtcggttgcg tgcctggcta
     4021 caacagcatc ggcatctggg gcgccgtgct gctcacgctg atccggctgg tccagggtat
     4081 cggcgtcggc ggggagtggg gcggatcggt gctgctcgcc atggagtggg cacagaccaa
     4141 caagaaccgt ggcttcctct cggcctggcc gcagttcggt gcgccggcgg ggctgttcct
     4201 ggcgaactgc gcggtgctgt tcttcagctg gtattcgggc gaccagttcc tggtctgggg
     4261 ctggcgcatc ccattcttcc tgagcgcgat catggtcgcc gtcggcctgt ggatccggct
     4321 tggcatcatg gagacgccgg tgttccagcg tatcctcgac gaggaacgca ccgagcgggt
     4381 gccggtgctg gaggtgctga agcggcagcc gaagcagatc atcctgacgg cgctgctgcg
     4441 catgccggaa caggcccctg gctatatcgt cggtgcctgg attttcacct acgcgaccgg
     4501 ggtgctgggg tattcgcgcg acttcgtact gcttggcgtc atctcgcaga ccgtgctggg
     4561 at
//
LOCUS       DATGEM010000064         9048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1233604, whole genome shotgun sequence.
ACCESSION   DATGEM010000064 DATGEM010000000
VERSION     DATGEM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233604"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..948
                     /locus_tag="VKQ27_01830"
     CDS             61..948
                     /locus_tag="VKQ27_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY87701.1"
                     /translation="MPLQTAGYAQLAVSVILLSSAWPLTKIALSFGSTPLYFAEGRAL
                     LSGATIAMILVLRGKLRLPTRADIPAVVAIGVGQLGLYFAFAHVAVAWVPAGRTAILA
                     NTTTIWVVPLSLMFLRERIPLRRWLACGFGVAGVVVLMSPWSIDWSSRNVLIGHAFLL
                     GASLSWSVAIIVTRTARPRLTMFQLLPWCFAISALVLAPLVAWHEPDGAIGANVQSWT
                     ALAYIGLIAGPFGTLCVILATAALPAVVSSVGFLTTPAVSIILSNLLLGEPITADLLA
                     GSALIMAGVGCAAWPGQRR"
     gene            945..1715
                     /locus_tag="VKQ27_01835"
     CDS             945..1715
                     /locus_tag="VKQ27_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY87702.1"
                     /translation="MRLQAIQIGEGSPPLVLLHGLFGSARNFGGVQRDFARHRRTIAL
                     DLRNHGASPHDADMRYASMAEDVVETLRELNGVPALLLGHSMGGKTAMQMALNHPDTV
                     ECLIVADIAPAPNPPVHLEVAMAMAALPLSPEMTRAQADAALAPAVPDAPMRAFLLQN
                     LQLGAAPAWRIGISEIIADFADIEAWDAPAGAQYTGPTLFIAGATSNYIKPEHRPIIR
                     SLFPSARFVTLKNAGHWLHADNPSGFVAVVEAFLGGAP"
     gene            1720..2946
                     /locus_tag="VKQ27_01840"
     CDS             1720..2946
                     /locus_tag="VKQ27_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HLY87703.1"
                     /translation="MRLNDEEQAMLAGKAGAACQWAIEHQMRVGKYFGAPDFVPVSQA
                     HIMADTESLGVAGVEWLERLAAQPGSQVRIPTITDPRGTDFAAAARLKQQEWMLALER
                     RAITAFQALGVLMTDTCINYQTIMPAVLGEHMAYGDTGVVIYSNSVLGARSNFEGGPS
                     ALAAGLTGRTPRYGYHLPELRRATLLVRVEYTPRALHDWGALGGIVGRIAGDYWQVPA
                     VVGIDRIPGSDELKHFGAALASYGSVALFHMAGVTPEADEAIDTALPAAASVTQADIA
                     AFLAAYRDGIDSVDVVVFSTPQLSLFELRTLAGLLDGRRTTIPLIAITSPQIKPDADR
                     SGITARIEAAGGMVLQGMCFYQSYAREMAEANGWRNLVTPSAKLVNILGGYGYRPALL
                     SMQECVDAACTGRIAA"
     gene            2943..3353
                     /locus_tag="VKQ27_01845"
     CDS             2943..3353
                     /locus_tag="VKQ27_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HLY87704.1"
                     /translation="MTEFRASAAMGKRVRGRALVAKDGFSARYDLDRVTGTFSRPEHK
                     LAGQSYVGRILVLDAAKGGVATAWMLHEMAARNVMPAALVLNRVNPIMVQGAALADFT
                     MISGFDVDITAAVPDGALVEIDPETAVLRVLADA"
     gene            3350..4234
                     /locus_tag="VKQ27_01850"
     CDS             3350..4234
                     /locus_tag="VKQ27_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY87705.1"
                     /translation="MSGRTSLANAERGLADLLPAIGAALTYSAGDILAKVVFNDGMDV
                     LSFITARGVLTVAFFWFWLRQAPPPRAHTSRERIVSVVVGLIFSANIFTLLLAVKLLP
                     LSVAILTYFTYPLFTGLTGAVTGIDRVHWRGLLAAFVAFLGLALMLETRLGDVSMLGI
                     VCALAASVGRTVTLLITRAALRGTDSRLNTWYSLLPSAGVFVLASLLVGSWSAPQTSL
                     GWVAFVGLCIATTIPVLLIFVAIARIGPFRTALIMNLEPLVSTLGSIVLLGEVMRPLQ
                     AAGGAIMLAALCAFQIRR"
     gene            complement(4301..5149)
                     /locus_tag="VKQ27_01855"
     CDS             complement(4301..5149)
                     /locus_tag="VKQ27_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HLY87706.1"
                     /translation="MSSASTEAESAADAHQLLSQRNRDGALARPAQWNAVLETILNHR
                     SVRGFLPKALPEGTLELLIAAAQSASTSSNLQFWSVVAVQEPERKSRLAELAGQQQFI
                     RDAPLLLVWLADMSRINRLAAERQAQVDGTHYVEEFIVGVVDAALAAQSALIAAESLG
                     LGGVYIGAMRNLPEQVAAELALPPHTFAVFGMSVGYPDPARETGIKPRLPQSVVLHRE
                     QYSTLQRSEAIDGYNATMREFQREQGMRAIDWTQQCFDRVKDGAALRGRDRMRKALRN
                     LGFELR"
     gene            5230..6492
                     /locus_tag="VKQ27_01860"
     CDS             5230..6492
                     /locus_tag="VKQ27_01860"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HLY87707.1"
                     /translation="MSIYTGPVFEMARDQFQVIADYLEIPSDERDRLLYPKRAIAVSC
                     PIHLDNGRLAVFQGYRVQHHLTLGPTKGGTRFALSVDIGEVAALAIWMSWKCALAGLP
                     YGGAKGGVTVDPRSLSRHELENLSRRYMQEMIPFVGPHTDVMAPDMGTNEQVMAWFMD
                     TYSMYQGRTVTEIVTGKPVASGGTEGRREATGRGVAHFISRALERINLAPRDATAIVQ
                     GFGNVGSYTVETLAQRGVKLIGVSDHTACYFDPRGLDAAALLTHAREHDGVLAGFSNQ
                     LSIDPRELLTMKCDVLVPAAMERVIDASVARRLQCRILAEAANGPTTPDADEVLNQRR
                     DEIFVIPDILCNAGGVVVSYFEWVQDLQQFFWDESEVMDKLYHVLDRAFSQVMRRVDR
                     DKVPHRTAAMAIGVEKVRSAKNTRGLFP"
     gene            6624..7985
                     /locus_tag="VKQ27_01865"
     CDS             6624..7985
                     /locus_tag="VKQ27_01865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87708.1"
                     /translation="MSRQIRLNAFDMNCVGHIQHGMWAHPRDRSSSYTDLDHWVSLAR
                     TLEAGLFDGLFLADILGVYDVFHDSPDASIRNAVQIPVNDPLLLIPAMAYATRDLGFG
                     VTCNLTYEPPYTFARRMSTLDHLTKGRIGWNVVTGYLDSAARGMGLAQQPEHDSRYEV
                     AEEYMQVAYQLWEGSWEDGAALHDRDARIYADPARIHRVKHDGPHYKVDAIHLSEPSP
                     QRTPVLYQAGASDKGRAFAAEHAECIFVNGQFRHNVRDIVADIRARAKGFGRDPHDIK
                     MFVGATVVVAPTEAEAQDKLAEYREYASSEGALAHYSASVGVDFSRYGPDEPIRFVKT
                     NALRSNLEAITVRSGSEWTLRKLMNMMVLGSRQAPIVGSPVQVADQMQAWVDEADVDG
                     FNLSRTVIPECVQDFIALVVPELQSRGLMKDRYGPGTLREKLFGPGRARVPVTHPAAR
                     FRR"
     gene            complement(8163..>9048)
                     /locus_tag="VKQ27_01870"
     CDS             complement(8163..>9048)
                     /locus_tag="VKQ27_01870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLY87709.1"
                     /translation="FERLWNADVIDHVQITVSETVGVEQRGGYYDHSGALRDMVQNHM
                     LQLLCLVAMEPPVSLDADRVRDEKLKVLRALRPIETHESTALTVRGQYTQGAIDGQPV
                     QGYIADLNGPESSSTETFVALKADVRTWRWANVPFYLRTGKRLPQKVSEIVIQYRALP
                     FSIFPEDAAEWQPNKLIIRLQPEEGMRLSMMTKDPGPGGLRLSPTALDIRFEKTFGMR
                     FPDAYERLLMDTVRGNATLFMRRDEVEAAWSWVEPILQAWSGRPDQPRPYPSGTWGPT
                     AAIALIERDGRTWHEDLA"
BASE COUNT         1439 a         2803 c         3028 g         1778 t
ORIGIN      
        1 gcagcggccg atgagtgaaa aggtcatgtg ttcctaatgt cgcgcccggt tgacgtatta
       61 atgccacttc agacggccgg gtatgcccag ctcgccgtgt cggtgatcct gctcagcagt
      121 gcttggccgt tgaccaagat cgccctgagt ttcggtagca caccgctgta ctttgcagaa
      181 ggccgtgcgc tgctgtccgg tgcaacgatt gccatgattc ttgtgttgcg gggcaagttg
      241 cgcctgccga cccgggccga tattcccgca gtggttgcga taggcgtcgg ccagcttggg
      301 ctctatttcg catttgcgca cgttgccgtc gcgtgggtgc cggcgggtcg gaccgcgatc
      361 cttgccaaca ccacaacgat atgggtcgtg ccgttgtcgc tgatgttcct gcgcgagcgc
      421 attccgctgc gccgctggct cgcctgcggc ttcggcgtag cgggcgttgt cgtcctgatg
      481 agtccgtggt cgatcgactg gtcgtcacgc aatgtcctga tcgggcacgc attcctgctt
      541 ggcgcgtcgc tgtcgtggtc ggtcgcgatc atcgtcaccc gcactgccag gccgcggctg
      601 acaatgttcc agttgctgcc ttggtgtttt gcgatcagcg cgctggtgct tgcaccgctg
      661 gttgcgtggc acgaaccaga cggcgcaatc ggggcaaatg tgcagtcgtg gaccgctctt
      721 gcctatatcg gcctgatcgc cggcccgttc ggcacgctgt gcgtcatcct ggcgacggct
      781 gcgttgccgg cggtggtatc gtccgtcggg ttcctgacca cgccggcggt cagcatcatc
      841 ctgtcgaacc tgcttctggg cgaaccgatc accgcggacc ttctggccgg gtccgcgctc
      901 atcatggcag gggtagggtg cgccgcatgg ccgggacagc gtcgatgaga ttgcaagcga
      961 tacagatagg tgagggaagt cctccgctcg tgctgctgca tggtctgttc ggatccgcgc
     1021 gcaacttcgg tggcgtgcag cgcgacttcg cacgacatag gcgcaccatc gcgcttgacc
     1081 tgcggaatca tggagcaagc ccgcacgacg ccgatatgcg ctatgcgagc atggccgagg
     1141 atgtcgtgga gacgctgcgc gagctgaacg gggtgccggc gttgctgctc ggccattcga
     1201 tgggcggcaa gaccgccatg cagatggcgc tgaaccatcc cgatactgtc gagtgtctga
     1261 ttgttgccga tattgcgccg gcgccgaacc ctccggtcca tctggaggtt gcgatggcta
     1321 tggcggcact gcctctgtcg ccggaaatga cccgcgcgca ggcggatgcg gcgctcgcac
     1381 cggcagtgcc ggatgcgccg atgcgtgcct tcctgctgca aaacctgcaa cttggcgcgg
     1441 cgccggcctg gcgtatcggc atttccgaga tcatcgccga ctttgccgat atcgaggctt
     1501 gggatgcgcc ggccggtgcg cagtacaccg gaccaacgct gttcatcgct ggtgcaacat
     1561 caaactacat aaagccggaa catcgcccca tcatccgcag cctgtttccc agtgcgcgct
     1621 tcgtcacgtt gaagaatgcg ggacattggc ttcatgccga caatccgtca ggcttcgtcg
     1681 ccgtggtcga agcgtttctg ggaggagcgc cctagtagca tgcgtctgaa tgacgaagag
     1741 caggccatgc tcgcgggcaa ggccggcgcg gcctgccaat gggcgatcga acaccagatg
     1801 cgtgtcggca agtacttcgg cgcgcctgac ttcgtgccgg tgtcgcaggc acacatcatg
     1861 gcggacactg agtcgcttgg tgtggccggg gtggaatggc tggagcggct tgccgcgcag
     1921 ccgggcagcc aggtgcgcat tccgaccatc accgacccgc gcggcaccga tttcgcagct
     1981 gccgcacggc tgaagcagca ggagtggatg ctggcactgg agcggcgcgc catcaccgca
     2041 ttccaggcgc tcggcgtgct gatgaccgac acctgcatca actaccagac gatcatgccg
     2101 gcggtgctcg gcgagcacat ggcgtatggc gacaccggcg tggtgatcta cagcaacagc
     2161 gtgctgggcg cgcgttcgaa tttcgagggc ggtccatcgg cgcttgctgc cgggcttacc
     2221 gggcggacgc cgcgctacgg gtatcacctg cccgagctgc gccgcgccac gctgctagtc
     2281 cgggtcgaat atacgccgcg ggcgctgcat gactggggag cgcttggcgg catcgttggc
     2341 cgcatcgccg gcgactactg gcaggtgccg gcggtggttg gcatcgatcg cattcctggc
     2401 tccgacgagc tgaagcattt tggcgccgcg cttgcgagct atggctcggt cgcgctgttc
     2461 catatggcgg gcgttacgcc ggaagcggat gaagcgattg atacggcgct gcctgcggcg
     2521 gcgagcgtta cccaggcgga catcgcggcg ttcctggccg cgtatcgcga tggtatagac
     2581 agcgtggacg tggtggtgtt ctccacacca cagctcagcc tgttcgagct gcgtacgctc
     2641 gcggggttgc tcgatgggcg ccgcaccacc attccgctga tcgcgatcac atcaccccag
     2701 atcaagccgg acgccgaccg ctccggcatc accgcgcgga tcgaggcagc cggcgggatg
     2761 gtactgcagg gtatgtgctt ctatcagagt tatgcgcggg aaatggccga ggcgaatggc
     2821 tggcgcaatc tcgtcacgcc atcggcgaag ctggtgaaca tcctcggggg gtacggctat
     2881 cggccggcgc tgctgtcgat gcaggaatgc gtcgatgccg catgcaccgg gaggatcgcc
     2941 gcatgaccga gttccgcgcc agcgccgcga tggggaagcg cgtgcggggg agggcacttg
     3001 tcgcgaagga tgggttttcc gcgcgctacg acctggatcg tgtcaccggc acgttttcgc
     3061 ggccggagca caagctggct gggcagtcct atgtcgggcg catcctggtg ctggatgcgg
     3121 cgaagggcgg cgtcgcgacg gcgtggatgc tgcatgaaat ggcggcgcgc aacgtgatgc
     3181 cggcggcact ggtgctcaac cgcgtcaacc cgatcatggt gcaaggcgct gcgcttgctg
     3241 acttcacgat gatctccggc ttcgatgtcg acatcacggc ggcggtgccg gatggggcct
     3301 tggtggagat cgatccggag actgcggttc tgcgcgttct tgctgacgca tgagtgggcg
     3361 gacatcactc gccaatgccg aacgcggcct ggctgacctg ctgcctgcca tcggcgctgc
     3421 gctgacctac agcgcaggcg atatcctcgc caaggtcgtg ttcaacgatg gcatggacgt
     3481 gctgtcgttc atcactgcgc ggggcgtgct gacagttgcc ttcttctggt tctggctgcg
     3541 tcaggcgccg ccaccacgag cgcacacgtc gcgcgagcgg atcgtgtctg ttgtcgtggg
     3601 tctgatcttc tccgccaata tctttacgct gctgctggca gtgaagctgc tgccgttgtc
     3661 ggtcgccatc ctcacctatt tcacctaccc gctgttcacc ggcctgaccg gcgctgtcac
     3721 cggcattgac cgcgtccatt ggcgcggact gctcgcggcg ttcgtcgcgt tccttggact
     3781 ggcgctgatg ctggagacgc ggctcggcga tgtatcgatg ctcggcatcg tctgtgcgct
     3841 cgcagcctcg gtggggcgca cggtcactct actgatcacc cgcgctgcgc tgcgaggcac
     3901 cgattcgcgg ctcaatacgt ggtattcgct gctgccttcc gccggcgtgt tcgtgctggc
     3961 gtcgctgctt gtgggcagct ggagcgcacc tcagacctcg ctcggatggg tagcttttgt
     4021 cggtctgtgc atcgcgacca cgatcccggt gctgctgatc ttcgtggcga tcgcgcgcat
     4081 cgggccattc cgcacggcgc tgatcatgaa tttggaaccg ctcgtctcaa cgctcggcag
     4141 catcgtgctg cttggcgagg tgatgcggcc gctacaggcg gcgggtgggg cgatcatgct
     4201 ggcggcgctt tgcgcgttcc agatacggcg gtgagttggg gcgcaaccgg atcgaggtgg
     4261 cttcgtcggc tgcgcctcct cgcaatggca agggtggcgg ttaccggagc tcgaagccaa
     4321 ggttgcgcaa tgccttgcgc atccggtcgc gtccgcgcaa tgcggcgccg tccttcacgc
     4381 ggtcgaagca ctgctgggtc cagtcgatcg cgcgcatgcc ctgttcacgc tggaattcgc
     4441 gcatcgtcgc gttgtagccg tcgatcgctt cgctgcgctg cagcgttgag tactgctcgc
     4501 gatgcagcac caccgattgc ggcagccgcg gcttgatgcc ggtttcgcgc gcgggatccg
     4561 gatagccgac agacatgccg aataccgcga aggtgtgggg tgggagggcg agttcggccg
     4621 ccacctgctc gggcagattg cgcatggcgc cgatatagac gccgccaagt cccagcgact
     4681 cggcggcgat cagcgcgctc tgcgctgcca gtgccgcgtc aaccacgccg acgatgaatt
     4741 cctcgacata atgcgtgcca tccacctgtg cctggcgctc ggcggcgagc cggttgatgc
     4801 gcgacatgtc ggcgagccac accagcagca agggcgcgtc gcggatgaac tgctgctggc
     4861 cggcaagttc cgccaggcgc gatttgcgct ccggttcctg caccgcgacg acgctccaga
     4921 actggaggtt cgacgatgtc gaggcggatt gcgccgcggc gatcagcagt tcgagcgttc
     4981 cctcgggcag cgcctttggc aggaagccac gtactgagcg gtgattgagg atcgtttcca
     5041 gcacggcatt ccactgtgca ggtcgagcca gtgcgccatc gcggttacgc tggctcaata
     5101 actgatgcgc atcggcggca gactccgcct cggtgcttgc ggatgacatc gggcactcca
     5161 gattccataa caattgttcc cgacccgtcc ggaccaggca acccggcggg cgctgatgga
     5221 ggcgtccaaa tgtcgatata tacgggcccg gtgttcgaga tggcccgcga ccagttccag
     5281 gtgatcgccg attatctgga gatcccgtcc gatgagcggg atcgcctgtt gtaccccaag
     5341 cgggcgatcg ccgtgtcatg cccgatccac ctggataacg gacgcctcgc ggtattccag
     5401 ggctatcgtg ttcagcatca tctgacgctc gggccaacca agggcggcac gcgcttcgcg
     5461 ctcagcgtcg atatcggcga ggttgccgcg ctcgccatct ggatgagctg gaagtgtgcg
     5521 ctggccggtc tgccctatgg cggggcgaag ggtggcgtca ccgtggatcc tcgttcgctg
     5581 tctcgccacg agctggagaa cctgtcgcgg cgttatatgc aggagatgat cccgttcgtg
     5641 ggcccccata ccgatgtcat ggcgccggac atgggcacca acgaacaggt gatggcctgg
     5701 ttcatggaca cctactccat gtatcagggt cgcacggtca ctgagatcgt caccggcaag
     5761 ccagtcgcct cgggtggcac cgaaggtcgt cgtgaggcta ccggccgcgg tgttgcgcat
     5821 ttcatcagcc gcgcgctcga gcgcatcaac ctggcacccc gcgacgccac cgcgatcgtg
     5881 cagggcttcg gcaatgtcgg cagctacacg gtagagacgc tggcgcagcg cggtgtgaag
     5941 ctgatcggcg tgtccgacca cactgcgtgc tacttcgatc cgcgaggtct cgatgcggct
     6001 gcgctgctga cgcacgcgcg cgagcacgat ggggtgttgg ctgggttttc caaccagctt
     6061 tcgatcgatc cgcgcgagct gttgacgatg aaatgcgatg tgctggtgcc ggctgccatg
     6121 gaacgcgtga tcgacgcgtc ggttgcccgg cggttgcagt gccgcattct ggccgaggcg
     6181 gcgaacggcc cgacaacgcc tgatgccgat gaggtgctga accagcggcg cgatgagatc
     6241 ttcgttatac cggatatcct gtgcaacgct ggcggggtgg tggtcagcta cttcgaatgg
     6301 gtgcaggatt tgcagcagtt cttctgggat gaatccgagg tgatggataa gctgtaccac
     6361 gttctggacc gcgccttcag ccaggtcatg cggcgcgtcg atcgtgacaa ggtaccgcac
     6421 cggacggctg cgatggcgat tggcgtcgag aaggtgcgca gcgccaagaa cacgcggggg
     6481 ctgttcccct gagtgataag taaacaggaa ccacagatgg acacagatgg acacagatca
     6541 ggccgttact ggaacactta tctgtgtcca tctgtgtcca tctgcggttc catctttctt
     6601 ttccgcttcg cgggttaagc ggcatgtcgc gccagatccg gctcaatgcg ttcgatatga
     6661 actgcgtcgg ccatattcag catggcatgt gggcgcatcc gcgcgatcgt tcgtcgagct
     6721 ataccgatct tgatcactgg gtctcgctgg cgcgcacgct ggaggcgggg ctgttcgatg
     6781 ggttgttcct ggcggacatc ctgggcgttt atgacgtgtt ccatgactcg ccggacgcct
     6841 cgatccgcaa tgccgtgcag atcccggtga atgatccgct gctgctgatc ccggcgatgg
     6901 cctatgccac gcgcgacctt ggtttcggtg ttacctgcaa cctgacctac gagccgccct
     6961 atacgtttgc tcgccgcatg tcgacgctgg accatcttac caaggggcgt atcggctgga
     7021 acgtggtcac cggctacctg gacagcgcgg cgcgcggcat ggggctggcg cagcagcctg
     7081 agcacgacag ccggtacgaa gtggccgagg agtatatgca ggtcgcctac cagctctggg
     7141 aaggcagctg ggaagatggg gccgcgctgc acgacaggga tgcgcgtatc tacgccgacc
     7201 cggcgcgcat ccatcgcgtc aagcacgacg gcccgcacta caaggtggat gcgattcatc
     7261 tgtcggagcc gtcgccacag cgcacgccgg tgctgtatca ggccggcgca tcggacaagg
     7321 ggcgcgcgtt cgccgcggag catgccgagt gcatcttcgt caacggccag tttcgccata
     7381 acgtgcgcga catcgtggcc gatatccgcg cgcgggccaa aggcttcggc cgcgacccgc
     7441 atgatatcaa gatgttcgtc ggggcgaccg tcgttgtcgc gcccaccgag gcggaggcgc
     7501 aggacaagct tgcggagtac cgcgaatatg ccagttccga gggggcgctg gcgcattact
     7561 ccgcctcggt cggcgtggac ttttcccgtt acgggccgga tgagccgatc cgcttcgtca
     7621 agaccaatgc gctgcgttcc aacctggagg cgatcaccgt tcgcagcggc agcgaatgga
     7681 cgttgcggaa gctgatgaac atgatggtgc tgggcagccg tcaggcacct atcgtcggct
     7741 cacctgtgca ggttgcggat cagatgcagg cctgggtgga tgaagcggat gtggacggct
     7801 tcaacctgtc gcgcactgtc atcccggaat gcgtgcagga ctttatcgcg ctggttgtcc
     7861 ctgaactgca atccaggggc ctcatgaagg atcgctacgg gccgggtacg ctgcgcgaaa
     7921 agctgttcgg tcctggccgg gcccgggtgc ccgtaacgca tccggccgcg cggttccgcc
     7981 gctaaaaaag tcgcggagct gtgaattacc tcacagaacc gtgcgggtag ccatgcgatc
     8041 aagcggtccg tcggtcggcg ctgccccaac acccgcgctc accggctggg ccacaggccc
     8101 aggtcaaccc ggaccttcct tggcggcctg gtcgtcctcc gacgaccggg ccgttttttt
     8161 ggtcacgcca gatcctcgtg ccaggtgcgt ccgtcgcgct cgatcagggc gatcgcggct
     8221 gtgggtcccc aggtgcctga cggatacggc cgaggctggt cgggccggcc gctccatgcc
     8281 tggagtatcg gctcgaccca ggaccatgcg gcctccacct cgtcgcgccg catgaacagc
     8341 gtcgcgttgc cgcgcacggt gtccatcagc agccgctcgt aggcgtcggg gaaccgcatc
     8401 ccgaaggtct tctcgaacct tatgtccagc gccgtcggcg aaagccgcag gccgccgggg
     8461 cccggatcct tggtcatcat cgaaagccgc atgccttcct cgggctgcag ccggatgatc
     8521 agcttgttgg gctgccattc ggccgcgtcc tccgggaaga tcgagaacgg cagggcgcgg
     8581 tactggatga cgatctcgga caccttctgc ggcagccgct ttccggtgcg caggtagaac
     8641 ggcacgttcg cccaccgcca tgtccgcacg tcggccttca gtgccacgaa tgtttcagtg
     8701 gagctgcttt ccgggccatt caggtcggcg atgtagcctt gcaccggctg accatcgatc
     8761 gcgccctggg tgtactggcc gcgcaccgtc agagcggtcg actcgtgtgt ctcgatcggg
     8821 cgcagggcgc gcagcacctt cagcttctcg tcgcgcaccc tgtcggcgtc cagcgacacc
     8881 ggcggctcca tggccacgag gcagagaagc tggagcatgt ggttctgcac catgtcgcgc
     8941 agcgcgcccg agtggtcgta gtagccgccg cgctgctcca cgccgacggt ctcgctgacc
     9001 gtgatctgga cgtggtcgat cacgtcggcg ttccagagcc gctcgaag
//
LOCUS       DATGEM010000065         9591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1691881, whole genome shotgun sequence.
ACCESSION   DATGEM010000065 DATGEM010000000
VERSION     DATGEM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691881"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VKQ27_01875"
     CDS             <1..430
                     /locus_tag="VKQ27_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87710.1"
                     /translation="SLLDYVHHLIGPAMVLALVTVAIWSRYMRSSMLEVTSQDFVRTA
                     TAKGLPRRVVIIRHALRNALLPMITLMGLQLPTLLGGALVTETIFTWPGMGRLFLDSI
                     GYRDYPVVMGLLMFTALLVLIGNLTADMLYALYDPRIRLD"
     gene            405..1289
                     /locus_tag="VKQ27_01880"
     CDS             405..1289
                     /locus_tag="VKQ27_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87711.1"
                     /translation="MTLASASTDAAVVPRRGGRGLRRFARHRLALIGACTIVFLILAA
                     GIGPYINPYDDLHIDILHRFQPPFVGPHLLGTDQLGRDMLARLEMAGRISLTVGFAAM
                     LLSIAFGTVVGMVAGFYGGLIGSVLMRLVDAVLCFPSIFLLLTLAAFVEPTLFTITVI
                     IAATAWMEVSRLVEAQIRALRDRDFAVAAVALGASDRMIMFRELIPNAIAAVIVAATL
                     NVARAILLESYISFLGYGIQPPVPSWGNMLNNAQEYLVSAPWLAIIPGAAITLAVTGF
                     NFLGDGLRDALDPRLDVR"
     gene            1286..2272
                     /locus_tag="VKQ27_01885"
     CDS             1286..2272
                     /locus_tag="VKQ27_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87712.1"
                     /translation="MSAVLEVSGLSVRFRTDDGVVPAVNDVSFTMGQGETLALVGESG
                     SGKSVTSLAIMRLTSGEVGGRVVLHDTDGATRDLLTLPESEMREIRGSRIAMIFQEPA
                     TSLNPVMTLGEQIAEPMRYHRSMGRRTALREAERLLDLVGIPDARRRLASYPHQLSGG
                     MRQRAMIAIALACEPAVLIADEPTTALDVTVQAQILELLRELQERTGMALLFITHNLG
                     VVAEIADTVVVMYAGRIVEKAPLRPLFERPLMPYTAGLMRSVPRLTGLRETLTAIPGN
                     VPDPALLPDGCSFAPRCDHLEAGRCDARVPELDAASDEHLVRCVRWRELAAP"
     gene            2269..3264
                     /locus_tag="VKQ27_01890"
     CDS             2269..3264
                     /locus_tag="VKQ27_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLY87713.1"
                     /translation="MTGETILTVEALSKLFALPRRSPLAARETVKAVDGVSFSVTAGE
                     VLGLVGESGSGKTTLGRTVLRLLEPSGGAIRFKDRDITHLSRRALQPIRREMQLVFQD
                     PFGSLNPRMTIGRIVATPLLIHRPELGRDQRREMVAAMLRRVGLLPAYAERFPHEFSG
                     GQRQRVGIARALILHPSFLVADEPVSALDVSIQAQVVNLLLELRAELGLSILFIAHDL
                     AVVGHISDRVAVMYLGKLVELAPTQALFDTPRHPYTEALLSAAPTPEPGRGRQRIMLA
                     GDPPSPVNPPSGCRFRTRCRYAEPACATAEPPLREVAAGHFSACIRAELTLSGAG"
     gene            complement(3560..4069)
                     /locus_tag="VKQ27_01895"
     CDS             complement(3560..4069)
                     /locus_tag="VKQ27_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HLY87714.1"
                     /translation="MAATPDDLFAFLDRLGIEHSTIEHPPFFTVEEGREWHDKIPGLH
                     CKNLFIKDRRGGIWLVVLPADKRADLGRLEKLLGAPRFSFARPDVLQEVLQLTPGSVT
                     PFGLINDTNRRATVVLDAAMFDSEWMNVHPLHNAASTTLRSADLLKFVRALGYEPIVV
                     RVPEVQTAE"
     gene            4152..5741
                     /locus_tag="VKQ27_01900"
     CDS             4152..5741
                     /locus_tag="VKQ27_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87715.1"
                     /translation="MHRRALLKSAATLVAMRGAVAAPAIAQPAKTATLRFVPQANLTL
                     LDPVFTTATVTSNHGYYVFDTLYSVGPDGESVPQMAEGHSVSDDKLTWRIRLRDGLWF
                     HDGSPVRATDCIASIERWSVRDPFGQLLAAAVDSYSAPDDRTIQVKLTKPFPLLDTAL
                     GKADSSVPFIMPERLAKTAADKPVTEMVGSGPYRFIANEYVNGSRVVYERFDKYVSRA
                     EQAMWGTGAKHAYYPRIVWHIIPDAATAGAALQNGEIDWWEQPLADLLPQLAANKEIE
                     LQVDQPWGRISWLILNHVQPPFNDVRIRRAVMMAVRQDDTMRATFGDDKSLWRVCKDV
                     FPFGTPYSSGQDGDIMQGDAKRAAQMLKEAGYSGQKVALMNPTDFAAIHPLGLVAADT
                     MKQMGLNVDLQETDWGTVVQRRSSMEPLEKGGWSAFHTFSSSVAVSTPATHPLTNGRG
                     PKGWFGWWDNAEARELTAQWVSASDSAAQAKAAKALSHVAMTDVATVPVGQWYGKTAF
                     RRSITGVLQGVSPYPWNVRPA"
     gene            5898..6341
                     /locus_tag="VKQ27_01905"
     CDS             5898..6341
                     /locus_tag="VKQ27_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87716.1"
                     /translation="MPDETSFVWPAAGAMQVTSLPSGGCNIKFTFADHGVVGSIGKGR
                     IARIREVPTRRIARQYEIIVRHEDGFESSYSIVDQTPHLRAGESGVLQMQGTLVEGGG
                     GLYDIRNGGSLHFQLYHAGKLVDPRMYIRGEPGSVGAPPQEAPGG"
     gene            6509..8458
                     /locus_tag="VKQ27_01910"
     CDS             6509..8458
                     /locus_tag="VKQ27_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY87717.1"
                     /translation="MTTPPNLYELLIHSVVDYAIFMLDPTGHIVSWNPGAEHIKGYTA
                     DEVIGQHFSRFYTEPDRAAGVPDRALRTAAETGRFAAEGWRVRKDGTLFWAQVLIDPI
                     RQDGRLLGFAKVTRDITEQRAAHLTALEAERRFRVLVRGVTDYAIYMLDPDGHVTNWN
                     AGADRIKGYAEHEIVGQHFSRFYTPEDVEAGIPWKALEQARREGRYESEGWRVRKDGT
                     RFWASAIIDAIHDDDGTLIGFAKITRDLTERREVQLELETAREQFFQAQKMEAVGQLT
                     GGLAHDFNNMLTGISGSLELLRVRIAQGRLADLDRYITSAQGAASRAAALTHRLLAFA
                     RRQTLDPKPIDANRLISAIEDLVQRTVGPQVKVETVLAIGLWATLCDPNQLESAILNL
                     CINARDAMPEGGQLTIETANTWLDARGARERDMEPGQYVAICVTDTGIGMSPDVLARA
                     FDPFYTTKPIGQGTGLGLSMVYGFAKQSHGQVRLYSEVGNGTTVRLYLPRHRGPAEIE
                     EELPRLEPVPRARSGETVLIVDDEPSVRMLVTDVLEELGYAAIEAADTISGLKVLQSD
                     VRIDLLITDVGLPGGMNGRQMADAARVTRPRLKVLFITGYAENAAIGNGRLEYGMHVM
                     TKPFAMDNLAARIKAIISEGNGSGQ"
     gene            complement(8530..9330)
                     /locus_tag="VKQ27_01915"
     CDS             complement(8530..9330)
                     /locus_tag="VKQ27_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87718.1"
                     /translation="MEHTAPADTSPDPQQDHPAITLSTNVDFLLHAARILLTYGCHLI
                     ETIRHRATTPSFNSIAANFGTANLSTILAHLKRGMLRATALERVLLARAATGRDIDFV
                     ERHKPEPQPATAEAQPQQSNAPEPATTGTHTPCPSRPAGWDDPELFMPTLEDLERQVR
                     RRPIGRTICDICLDLAVVPGLCHSAFWNELFDIMNWFGGGNSVDRLMRTKARRREAFA
                     NEQDRNPKASWDWLRPKRDELRQILGFFIGEPPVNPCEPAAAVATGPP"
     gene            9440..>9591
                     /locus_tag="VKQ27_01920"
     CDS             9440..>9591
                     /locus_tag="VKQ27_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY87719.1"
                     /translation="MTDLSSPEFHDETKAREWLELARWPTGAYCPHCGSFDVFRMEGQ
                     PERRQRG"
BASE COUNT         1620 a         2807 c         3445 g         1719 t
ORIGIN      
        1 gtcgctgctc gactacgtgc accatctgat cggaccggcg atggtgctgg cactggtgac
       61 cgtggcgatc tggagccggt atatgcgttc gtccatgctg gaggtgacca gccaggattt
      121 cgtgcgcacg gcgacggcga agggattgcc gcgccgggtg gtgatcatcc ggcacgcgct
      181 gcgcaatgcg ctgctgccga tgatcacgct gatggggctg cagctgccga cactgctcgg
      241 cggcgcgctg gtcaccgaga cgatcttcac ctggccgggg atgggacggc tgttcctgga
      301 ttccatcggc tatcgcgact atccggtggt gatgggcttg ctgatgttca ccgcgctgct
      361 ggtgctgata ggtaacctga cggcagacat gctctacgct ttgtatgacc ctcgcatccg
      421 cctcgactga cgcggccgtt gtgccgcgcc gcggtgggcg ggggctgcga cggttcgcgc
      481 ggcaccggct ggcgctgatc ggggcgtgca cgatcgtgtt tctgatcctg gcggcgggga
      541 tcgggcctta catcaatccc tatgacgatc tgcatatcga catcctgcat cggttccagc
      601 cgccattcgt gggaccgcat ctgctgggaa ccgatcagct cgggcgcgac atgctggcgc
      661 ggctggaaat ggcggggcgg atttcgctga cagtggggtt tgccgcgatg ctgctgagca
      721 tcgcgttcgg caccgtcgtc ggcatggttg ccgggttcta tggcgggctg atcggttccg
      781 tgctgatgcg gctggtagat gccgtgctgt gctttccctc gatcttcctg ctgctgacct
      841 tggcggcgtt cgtcgaaccg acgctgttca ccatcacggt gatcatcgcg gcgacagctt
      901 ggatggaagt gtcgcggctg gtcgaggcgc agatccgcgc gctgcgggat cgcgatttcg
      961 cggtggccgc tgtggcgctg ggcgcgtccg accggatgat catgttccgc gagctgatcc
     1021 cgaacgcgat tgccgcggtg atcgtggcgg cgaccctgaa tgtggcgcgg gcgatactgc
     1081 tggaatcata tatcagcttc ctgggctacg gcatccagcc gccggtgcca agctggggga
     1141 acatgctgaa caacgcacag gagtatctgg tgtcggcacc atggctggcg atcatcccgg
     1201 gggcggcgat cacgctggcg gtgacggggt tcaacttcct gggcgacggg ctgcgtgacg
     1261 cgctcgatcc tcggctggat gtgcgatgag cgcggtgctg gaggtctcgg ggctgtcggt
     1321 gcggttccgt acggatgacg gcgtggtacc ggcggtcaac gacgtgtcgt tcaccatggg
     1381 acagggtgag acgctggcac tggtgggcga gtcgggatcg gggaagagcg tcacgagtct
     1441 ggcgatcatg cggctgacgt ccggcgaggt gggcgggcga gtcgtgctgc acgataccga
     1501 cggcgcgacg cgcgatctgc tgacgctgcc agagtcggag atgcgcgaaa tccgcggcag
     1561 ccggatcgcg atgatcttcc aggagccggc gacgtcgctg aacccggtga tgacgctggg
     1621 tgagcagatc gccgagccga tgcggtatca ccggagcatg ggtcgcagga cggcgctgcg
     1681 cgaggcggag cgtctgttgg atctggtcgg cattccggac gcacggcgca ggctggcgag
     1741 ctatccgcat cagctttccg gcggcatgcg ccaacgggcg atgatcgcga tcgcgctggc
     1801 atgcgagccg gcggtgctga tcgcggatga acccaccacg gccctggatg tcacagtgca
     1861 ggcgcagatc ctggagctgt tgcgcgagtt gcaggaacgg accggcatgg cgctgctgtt
     1921 catcacgcac aatctcggcg tggtggcgga gatcgccgat acggtggtgg tgatgtacgc
     1981 cgggcggatc gtggagaagg cgccgctgcg gccgttgttc gagcgtccgc tgatgcctta
     2041 cacggcgggg ctgatgcggt cggtgccgcg gctgaccgga ctgcgggaga cacttaccgc
     2101 gatccccgga aatgtacctg acccggcgct gctgcctgat ggctgcagct tcgcgccgcg
     2161 gtgcgaccac ctggaggccg ggcggtgtga tgcgcgtgtg ccggagcttg atgcggcgag
     2221 tgacgaacat ctggtgcgtt gtgtgcgctg gcgcgagctg gctgcgccgt gacgggcgag
     2281 accatcctga cggtcgaggc gctgagcaag ctgtttgcgt tgccgcgtcg atcgcctttg
     2341 gcagcgcgcg agacggtgaa ggcggtggat ggggtttcct tcagcgtgac ggctggtgag
     2401 gtactggggc tggtcggcga atccgggtcc ggcaagacga cgctcggccg caccgtgctg
     2461 cgtctgctgg agccgagcgg cggggcgatc cggttcaagg accgcgacat cacgcatctg
     2521 tcgcgacgcg cgttgcagcc gataaggcgc gagatgcagt tggtgtttca ggacccgttc
     2581 gggtcgctga atcctcggat gacaatcggg cggatcgtgg cgacgccgct gctgatccat
     2641 cggccggagc ttgggcgcga tcagcggcgc gagatggtgg cggcgatgct gcggcgggtg
     2701 ggactgctgc cggcatacgc ggagcggttt ccacatgagt tctccggtgg gcagcggcag
     2761 cgtgtgggga ttgcccgcgc gctgatcctg catccgagct ttctggtggc ggacgagccg
     2821 gtgtccgctc tggatgtatc gatccaggcg caggtggtga acctgctgct ggagctgcgc
     2881 gcggagctgg ggctgtcgat cctgttcatc gcgcatgatc tggcggtggt ggggcatatc
     2941 tccgatcggg tcgcggtgat gtatctgggg aagctggtgg agctggcgcc gacgcaggcg
     3001 ctgttcgata cgccgaggca tccttacacc gaggcgctgt tgtcggcggc gccgacgccg
     3061 gagccggggc ggggacgaca gcgcatcatg ctcgcgggcg atccaccgag tccggtgaac
     3121 ccgccatcgg ggtgccggtt ccggaccagg tgccgatatg cggagccggc ctgtgcgacg
     3181 gcggagccac cattgcgcga ggtggcggcg gggcatttca gcgcgtgtat acgggcggag
     3241 ctgacgctga gcggggcggg atgatcaccg tgatcacagc tctggcgaag gccggcggaa
     3301 gcggcattgg atgcgttcgg agcgcaccga ttcaccgata tggtaacggt ctcactcctc
     3361 ggaacgtgcc cgaaagagga ttcaacgcaa aggccgcgaa gaggcgcaaa gatcgccgag
     3421 aaggattcgt cttgagcctt cggcctatgg cgatgcagca gcgaacaggc tctgcgcggt
     3481 cttcgcgttc gttcccgggg ggccggctcg aacggcttcg cctcgctcgc aatgactggc
     3541 caggcagttg gccgtcgctt cactcggctg tctgaacttc cggcacccgc acaacgatcg
     3601 gctcgtaacc gagcgcgcga acgaacttca gcaggtcggc ggatcggagt gtggtcgagg
     3661 cggcgttgtg cagggggtgg acgttcatcc attcgctgtc gaacatcgct gcgtcgagga
     3721 cgacggtcgc gcgccgattg gtgtcgttga tcaggccgaa cggcgtcacg gagccgggcg
     3781 tcagttgcaa gacttcctgc aatacgtcgg gccgggcgaa ggaaaagcgt ggcgcgccca
     3841 gcagcttttc caggcgacca aggtcggcgc gcttgtccgc cggcaggacc accagccaga
     3901 tgccgcccct gcggtccttg atgaacaggt tcttgcagtg caggcccggg atcttgtcgt
     3961 gccactcgcg tccctcctcc acggtgaaga agggagggtg ctcgatcgtg ctgtgctcga
     4021 tgcccagccg atcgaggaac gcgaagaggt cgtcgggggt tgctgccatg acggtccgat
     4081 gttgccagcg gccgtcatcg ggctgcaatg tacgagccag acgaaaatcg aaacgtatct
     4141 gggaggtcgc aatgcaccgc cgcgcgctgt tgaagtccgc tgcaacgttg gttgcgatgc
     4201 gcggcgcggt ggcagcaccc gcgatcgcgc agccagccaa gacggcgacg ctgcgattcg
     4261 tgccacaggc gaatctgacg ctgctcgacc cggtgttcac caccgcgacc gtcaccagca
     4321 accatggcta ctacgtgttc gacacgctgt attccgtcgg accggacggg gaatcggtgc
     4381 cgcagatggc ggaaggacat tcggtttccg acgacaagct gacatggcgc atccgtctgc
     4441 gcgacgggct gtggttccat gacggttcgc cggtgcgcgc gaccgattgc atcgccagca
     4501 tcgagcgctg gtcggtacgc gatccgttcg gccagttgct cgctgctgcg gtggacagct
     4561 actcggcacc tgatgatcgg acgatacagg tcaagctgac gaagccgttt ccgctgctcg
     4621 atacggcgct tggcaaggcc gattcgtccg ttcccttcat catgccggaa cgcctggcca
     4681 agaccgctgc ggacaagccg gtgaccgaga tggtgggctc gggcccttac aggttcatcg
     4741 ccaacgaata cgtcaacggc agccgcgtgg tgtacgagcg gttcgacaaa tatgtctcgc
     4801 gcgccgagca ggccatgtgg ggcacgggcg ccaagcacgc gtattatccg cgcatcgtct
     4861 ggcacatcat tccagacgcc gcgacagccg gtgcggcgct gcagaacggc gaaatcgact
     4921 ggtgggagca gccgctggct gatctgctgc cgcaacttgc ggccaacaag gagatcgagc
     4981 tgcaggtgga ccagccctgg ggccgcattt cctggctgat cctcaaccac gtgcagccgc
     5041 cgttcaatga tgtgcgcatc cgccgtgcgg tgatgatggc ggtgcggcag gatgacacga
     5101 tgcgggccac gttcggcgac gacaaatcgc tgtggcgcgt ctgcaaggac gtgttcccat
     5161 tcggcacgcc atactcgagc ggccaggacg gcgacataat gcagggcgat gcgaagcgcg
     5221 cggcgcagat gctgaaggag gcaggctact ccgggcagaa agtggcgctg atgaatccga
     5281 cggacttcgc ggcaatccat ccgctcggcc tggtcgccgc ggataccatg aagcagatgg
     5341 gcctgaatgt tgatctgcag gagacggatt ggggcaccgt ggtgcagcgc cgctccagca
     5401 tggagccgtt ggagaaaggt gggtggagcg cgttccatac tttctcctca tcggtggcgg
     5461 tgagcacgcc ggcgacgcat ccgctgacca acgggcgcgg cccgaaaggc tggttcggct
     5521 ggtgggacaa cgccgaagcg cgcgagctca ccgcgcaatg ggtgagcgca tcggactccg
     5581 cggcacaggc gaaggcggcg aaggcgctgt cgcatgttgc gatgacggat gtggcgaccg
     5641 tgccggttgg gcaatggtac gggaagaccg cgttccgccg cagcatcacc ggcgtgctgc
     5701 aaggggtctc gccgtacccc tggaatgtac ggccagctta gcgggcggga cgcttgacgg
     5761 ggcgggcgca tcggtgaagg tgggaagtgc cggaccggga ggaggcggtg cgccggctcg
     5821 aatggtggct gtcctctggc cttccgccgc gatagcctcc gccatggcct gggaatcttg
     5881 gtgttgggga atgaaacgtg cctgacgaaa catcgttcgt gtggccagcg gcaggggcca
     5941 tgcaggtgac atcgttgcca agtggtggtt gcaacatcaa attcaccttc gccgatcatg
     6001 gcgttgtggg atcgatcggc aagggacgca tcgccaggat ccgcgaggtg ccgacgaggc
     6061 ggatcgcccg gcaatacgag atcatcgtcc gccacgagga cgggttcgaa agcagctaca
     6121 gtatcgtgga tcagacgccg catttgcgcg ccggcgagtc cggggtgctg cagatgcagg
     6181 ggacgctcgt tgagggcggt gggggactgt acgacatcag gaatggcggg agcctgcatt
     6241 tccagcttta tcacgccggc aagctggtgg atccgcggat gtatatacga ggggaaccgg
     6301 gcagcgttgg ggcgccgccg caggaagcgc ccggagggtg acggctgcac gccgagtcga
     6361 aggtaaggaa tggggcaaca gaaccgctgc caaagggcgg ggtaacagca caatcgtgca
     6421 cgctctcgca caatagtgac ccgccatcct ggcatacatg tccgaacagt acggtagcgt
     6481 acatcggttg acggacaggc ggagcgagtt gaccaccccc ccgaaccttt atgagctgct
     6541 gatccacagt gtcgtggact atgcgatctt catgctcgac ccgaccggcc acatcgtgag
     6601 ctggaaccct ggcgccgagc acatcaaggg ctatacggcc gacgaagtca tcgggcagca
     6661 cttctcccgc ttctacaccg aacctgaccg cgccgccggc gtcccagacc gtgccctgag
     6721 gacagcggcg gaaaccggcc gcttcgccgc agagggctgg cgtgtccgca aggacggcac
     6781 cttgttctgg gcgcaggtgc tgattgaccc gatccgccag gacggccgac tgcttggctt
     6841 cgccaaagtg acccgcgaca ttaccgagca gcgcgcggcc catctcacgg cgctggaagc
     6901 cgagcgccgc ttccgcgtgc tggtgcgcgg cgtcaccgat tacgccattt acatgctgga
     6961 cccggacggc catgtcacga actggaatgc cggtgcggac cggatcaaag gctatgccga
     7021 acacgaaatc gtcggccagc acttctcccg gttctatacg cccgaggatg tcgaagccgg
     7081 cattccctgg aaggcactgg agcaggctcg ccgtgagggc cgctatgaat ccgagggctg
     7141 gcgggttcgg aaagacggca cgcgcttctg ggcaagcgcc atcatcgatg cgatccacga
     7201 cgacgatggg accctgatcg ggtttgccaa gattacgcgc gacctgaccg agcggcgcga
     7261 ggtccagctc gaactggaaa cggcgcgcga gcagttcttc caggcgcaga agatggaggc
     7321 tgttggccag ctcaccggcg gtctggcgca cgacttcaac aacatgctga ccggcatctc
     7381 cggcagcctg gagctgttgc gcgtgcgcat tgcgcaaggc cggctggcgg atctcgatcg
     7441 ctacatcacc agcgcccagg gcgccgcatc gcgcgcagcg gcgctgacgc atcgtctgct
     7501 ggcgttcgcc cgccggcaga cgctggatcc gaagcccatc gacgccaacc gtctgatctc
     7561 ggccatcgag gatctggtcc agcgcaccgt cgggccgcag gtgaaggtgg agacggtgct
     7621 ggccatcgga ctctgggcca cactctgcga tcccaaccag ctggaaagcg cgatcctcaa
     7681 cctgtgcatc aacgcgcgcg acgcgatgcc ggagggcggc caactgacca tcgaaacggc
     7741 caatacgtgg ctcgatgccc gcggcgcccg cgagcgcgac atggaacccg gccagtacgt
     7801 ggcgatctgc gtcaccgaca cgggtatcgg catgtcgcct gacgtactcg cgcgggcctt
     7861 cgatcctttc tacaccacca agcccattgg ccagggcacc ggcctcggcc tttctatggt
     7921 ctatggtttt gccaaacagt cgcacggcca ggtgcgcctc tattcggagg tgggcaatgg
     7981 caccacggtg cggctgtatc tgcctcgtca ccgcggccct gccgagatcg aggaggaact
     8041 gccccggctc gagccggtgc cgcgcgccag gtccggcgag acggtgctga tcgtggatga
     8101 cgaaccgagc gtgcgcatgc tggtgaccga tgtgctggaa gagcttggct atgccgcgat
     8161 cgaggctgcc gacaccatct cgggcctgaa ggtgctgcaa tccgatgtgc gcatcgatct
     8221 gctgatcacg gacgtggggc ttccgggtgg catgaacggc cgccagatgg ccgacgctgc
     8281 gcgcgtgacg cgcccgaggc tgaaggtgct gttcatcacc ggctatgccg agaatgcggc
     8341 gatcggcaac ggacggctgg agtatgggat gcacgtgatg accaagccat tcgcgatgga
     8401 caatctggcc gcccggatca aggcgatcat cagcgaaggg aatggcagcg gccaatgaag
     8461 gcgaaccatg gccccgttgg ccgtgcgcct tcgcccgacg ttgtcggcgt tgaatcgctg
     8521 atcgggggat cagggcgggc cggtggcgac cgccgctgca ggttcgcacg ggttgaccgg
     8581 cggttcgccg atgaagaagc cgaggatctg gcgcagctca tctcgtttcg gacgtaacca
     8641 atcccagctc gctttgggat tgcggtcctg ctcgttggcg aatgcctcgc gacgccgggc
     8701 tttcgtgcgc atcagcctgt ccacgctgtt gccgccgccg aaccagttca tgatgtcgaa
     8761 caattcgttc cagaaggcgc tgtggcagag gccgggcacg acggcgagat cgaggcagat
     8821 gtcgcagatg gttcggccaa tcgggcggcg gcgcacctga cgctcgaggt cttcgagcgt
     8881 gggcatgaag agttctggat catcccagcc ggccgggcgg gatgggcacg gcgtgtgcgt
     8941 gccggtggtg gctggctcgg gcgcgttcga ttgctgaggc tgggcttcgg ccgtggcggg
     9001 ctgaggttct ggcttgtggc gctcgacgaa gtcgatgtcg cggccggtgg cggcgcgggc
     9061 gaggagcacg cgctcgagcg ccgtggcgcg cagcatgcct cgtttcaggt gggcgaggat
     9121 ggtggacagg ttggccgtgc cgaagttggc ggcgatggag ttgaagctgg gtgtggtggc
     9181 gcggtgccgg atggtttcga tcaggtggca gccataggtg agcaggatcc gggcggcgtg
     9241 gagcaggaaa tcgacgttgg tggacagagt aatggcgggg tgatcctgct ggggatcggg
     9301 ggaggtgtcg gccggtgcgg tgtgctccat tggtagtgac tctatcggag cgttaggtgt
     9361 gtgtcaagga aaaattaccc aattatccct ttgacaaagc ggcgcagtag gaatataaga
     9421 tcggtcaagg agttagccaa tgaccgacct ttcctccccc gaattccatg acgagaccaa
     9481 ggctcgcgag tggctggaac tggcacgctg gccgactggc gcttactgcc cgcactgcgg
     9541 ctcgttcgac gtgttccgta tggagggcca gcctgagcgc cgtcagagag g
//
LOCUS       DATGEM010000066         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_785961, whole genome shotgun sequence.
ACCESSION   DATGEM010000066 DATGEM010000000
VERSION     DATGEM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785961"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VKQ27_01925"
     CDS             <1..1791
                     /locus_tag="VKQ27_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87720.1"
                     /translation="DIHFDIWNQAGVETTGDIPVSIGTVDPIDTWNGSVSSDWNTGAN
                     WSTGAAPVSGDSVVIPAGTPFNASLTDATLTGETITVSGSATVDFNNVTLDSILQTAD
                     TGNIILGGTLTVGAQGSLGPEQHGSLFVRSLGSAVPIVNDGVIEAPAGSLLTFNNGGT
                     VTSASVTISNNGLISANGGDVSFDFAPPPFGTAPPETLNNSGSISIANGGGLTLNGTF
                     VGNDVAFAGPGALALQSPDAFAGGAAVTGFGQGDEIDLYSTAQGAPLGYGNGTLTAGT
                     STSIPLPGTFGVGNFVYEFTGGGQSPERIAYAPSGGPSGLDHPDITAPGTATIAQGST
                     LSLSDVSISGSGTADDTMSIKAGTGTLFMNGASGSGTSLLSLGPLPESQLNADLASLT
                     YVPAAGATSDILQVEVSPPAPVSTYREIPIAITPGEQDPVLSEPANEAVAPGAMVAVS
                     GGYSDSFAQHNPGQLFIGVGDTAGTLTATVASGAAAAGSGTNSIALSTDYVDLNAILA
                     SLHYTAGASAGSDTIQFQVWNQAGVETTAATNVTIGSAVSGTALGPADFAAQSATSVG
                     ATTLPATPGLMGMSGDAASPPPPMAALFSH"
     gene            1883..3892
                     /locus_tag="VKQ27_01930"
     CDS             1883..3892
                     /locus_tag="VKQ27_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLY87721.1"
                     /translation="MSLNSLIENLSIRNKIAACFAVILAIVVVLGAASVSRLTTLNRT
                     VDALTGDAIQGVSELSNMREALLRYRLAVARYMAGDKFTTDFNAAIDKALATYREHEA
                     KYASSVTSPEEQALYRKVQDTMRDYLESVAPTITLYDAAKPAEAWKLYQANGAITRGE
                     TVDVALANDKDYNAAMADRLTGQANADYHTGFWIVGGLLAAAIVLAVAAGFVLVRNIA
                     HPLVRSSQVLDQLARRDYDFELRQAHRRDEIGALSGAMDKLRNALQEGDRLAAAQQAA
                     QSAKARRQAAMEQHTQDFGNSISGVMASLASSAEAMRRAAEAMSEAASTVHSEASDTS
                     SGAAKASGDLSAVAASVEQLNHSVAEISRQLAEATAVAQQAVQRADTSHATMQGLSEA
                     TVRIGDVVHLISDIASQTNLLALNATIEAARAGEAGKGFAVVAGEVKTLAAQTAKATS
                     EIATQIEMVRTATGEAVTAMADIGGIIARINEVSAAIAAAVEEQSATTREIAASVQAV
                     AEATAGTAQAMQQVVTVSDNAGSISRDVLAGAADIGNEAKNLREEVDQFLVAVRDETS
                     DERRRYERVRTTGITAVVQAKGRSPARMDLRNVSRGGAALASDWRSPSGTALDVELPD
                     AGGPVPARVVRSGNGELAVVFSSEPRALARIDQALAALTGTKRAA"
     gene            3989..>5599
                     /gene="lepA"
                     /locus_tag="VKQ27_01935"
     CDS             3989..>5599
                     /gene="lepA"
                     /locus_tag="VKQ27_01935"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HLY87722.1"
                     /translation="MTPLDHIRNFAIIAHIDHGKSTLADRLIQTTGALSDREMTNQVL
                     DNMDLERERGITIKAQTVRLTYEAKNGQVYELNLMDTPGHVDFSYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYQAIEANHEIVPVLNKIDLPAAEPARVKQQIEDVIGLDATD
                     AVEISAKTGLNIEGVLEALVHRLPPPKGDDNGPLKALLVDSWYDQYLGVVILIRVKDG
                     RVKRGQRIRMMAKGSVHQVEQVGVFSPKMIPVDDLGPGEIGYITAAIKTVADCSVGDT
                     ITDDRTPATEPLPGFKPSVPVVWCGLYPVDADDFEKLRDSLAKLRLNDASFHYEPETS
                     AALGFGFRCGFLGLLHLEIIQERLTREFDLDLIATAPSVVYHIHRSNGTMVELHNPVD
                     MPDGSVIDHIEEPWIRATIMVPDDYLGAVLQLCNERRGEQVDLTYVGNRAMAVYRLPL
                     NEVVFDFYDRLKSVSRGYASFDYQMDGYAEGDLVRISILVNSDPVDALSFIAHRSVAE
                     ARGRSICAKLKDLIPRQLFKIAIQAAIGG"
BASE COUNT         1057 a         1835 c         1770 g          937 t
ORIGIN      
        1 gacatccact tcgacatctg gaaccaggcc ggcgtcgaga ccaccggcga cattccggtc
       61 agtattggca cggtggatcc gattgacacc tggaacggca gtgtcagttc cgactggaac
      121 acgggcgcga actggtccac cggcgccgcg cctgtcagcg gtgacagcgt ggttatcccg
      181 gccggcactc ccttcaacgc cagcctcacc gacgcaacgc tgaccgggga gaccatcacc
      241 gtcagcggca gcgccacggt ggatttcaac aacgtcaccc tcgactcgat tctgcaaacc
      301 gccgacacgg gcaacatcat tctaggcggc acattgactg tcggtgcaca aggttcgctc
      361 gggccggagc agcacgggtc gctgttcgtc cgcagccttg ggtctgcggt gccaatcgtc
      421 aatgacggcg tgatcgaggc gcccgctggt tctctcctga ccttcaataa cggtggcaca
      481 gtcacaagtg ccagcgtgac gatcagcaac aacggcctca tctcggcgaa cggcggcgat
      541 gtaagtttcg acttcgcgcc gccgccattc ggcaccgcgc cgccggagac gctgaacaac
      601 tctggatcca tcagcatcgc caatggcggc ggcctgacgc tgaacggtac tttcgtcggc
      661 aacgacgttg cctttgctgg gcctggcgcg ctggcgttgc agtcgccaga tgcattcgcc
      721 ggcggcgcgg cggtgaccgg tttcggacag ggcgacgaga tcgacctgta cagcaccgcg
      781 caaggtgcgc ctcttggtta cggcaacggc acccttaccg ctggaacgtc cacctcgatt
      841 ccccttcccg gcacgttcgg cgtcggtaac ttcgtctacg aattcaccgg cggcggccag
      901 agccctgagc gcatcgctta tgcaccgtca ggcggaccca gcggcctcga ccatccggac
      961 atcacagcgc cgggtactgc aacaatcgcc caaggctcga ctctgtcact aagcgatgtc
     1021 tcgatcagtg gatcgggtac cgcagatgac accatgagca tcaaggcggg gacaggcacc
     1081 ttgttcatga atggcgcttc gggatcgggc accagtttgc tgtcccttgg cccgttgcct
     1141 gaaagccagc tcaatgcaga ccttgcgagt ttgacttacg tccctgcggc aggcgcgacc
     1201 tccgacatat tgcaagttga agtgagcccg cccgcgccag tatcgacgta ccgcgaaatc
     1261 ccgattgcga taacgcctgg cgagcaagac cccgtactga gcgaaccggc aaatgaggct
     1321 gtcgctcctg gtgccatggt cgcggtcagc ggcggctatt ccgacagctt cgctcagcac
     1381 aatcctggcc agttgttcat tggcgtcggc gacaccgccg gcacgctaac tgccacagtt
     1441 gcctccggcg cggcggccgc gggatctggc accaacagca tcgccctctc cacggactat
     1501 gtcgatctca acgcgatcct ggccagcctc cactataccg ccggcgcgag cgctggatcc
     1561 gacacgatcc agtttcaggt ctggaaccag gctggtgtcg agaccactgc cgccacaaat
     1621 gtgacgatcg gaagcgccgt gtcgggcacc gcgctagggc cggcggactt cgccgcgcag
     1681 tctgctacat ccgtcggtgc caccacgctg cccgcaacgc ccggcctcat gggcatgtcc
     1741 ggcgatgcgg cgagcccccc gccccccatg gcggcactct tcagccactg acccgatcat
     1801 taagccttcc gtaagcctgt tcggcgcaat actgccggac agcgcgccag gccgcctgtg
     1861 cgcgccggaa gggctggcat cgatgtcctt gaattctttg atcgagaatc tgtccatccg
     1921 gaacaagatc gccgcttgct tcgcagtcat cctggcgatt gtcgtggttc tcggcgccgc
     1981 ctcggtgagc aggctgacca cgctgaaccg tacggtcgac gcactgaccg gcgacgccat
     2041 ccaaggcgtc agcgaattga gcaacatgcg tgaagcgctg ttgcgctacc gcctcgcggt
     2101 tgccaggtat atggcgggtg acaaattcac aactgatttt aacgccgcaa tcgacaaggc
     2161 gctggcaacc tatcgagagc acgaggccaa atatgcatcc tccgttacct caccggagga
     2221 acaggcactc taccgcaaag tccaggatac aatgcgggat tacctggagt cggtggcgcc
     2281 cacgatcacg ctgtatgacg cggcaaaacc agcagaagca tggaagctct atcaggccaa
     2341 cggcgctata acccgtggcg agacagtcga tgtcgcgctg gcaaatgaca aggactacaa
     2401 tgccgcaatg gcggaccggc tgaccgggca ggccaacgcc gattaccaca cgggcttctg
     2461 gattgtcggc ggattgctcg ctgctgctat cgtccttgct gttgccgccg gctttgtgct
     2521 ggtccgtaac atcgcccacc cattggttcg gtcgtcacaa gtgctggacc agctcgcccg
     2581 gcgcgattac gacttcgagc tgcgtcaggc acaccggcgt gacgagatcg gcgccctgtc
     2641 gggcgccatg gacaagctgc gcaatgctct gcaggaaggc gatcggctcg ccgccgcgca
     2701 gcaggccgcg cagtcggcca aggctcgccg gcaggcggcg atggagcagc acacacagga
     2761 tttcggcaac tcgatctccg gcgtcatggc gtcgctcgcc agctccgccg aagcgatgcg
     2821 gcgcgctgcc gaggcgatgt cggaggccgc cagcacggtc cactcggagg cgtcggatac
     2881 ctcatccggc gccgccaagg catcgggtga tctgtctgcc gttgccgcgt cggtcgagca
     2941 gctcaaccac agcgtcgcgg aaatatcgcg ccagttggcc gaggcaaccg cggtggcgca
     3001 acaggccgtg caacgcgccg ataccagtca tgccaccatg caggggctct cggaggcgac
     3061 agtgcgcatt ggcgatgtgg tgcatctgat cagtgacatt gcaagccaga ccaacctgtt
     3121 ggcgctgaac gcgacgatcg aggcggcgcg cgccggcgag gccggcaagg gctttgccgt
     3181 ggtcgcggga gaggtgaaga cgctggcggc acagaccgcc aaggcgacct cggaaattgc
     3241 cacgcagatc gagatggtgc ggaccgcgac aggtgaagcg gttaccgcaa tggccgatat
     3301 cggcggtatt atcgcccgga tcaatgaagt ttcggctgcg atcgccgccg ccgtggagga
     3361 gcagagcgcg accacgcgcg agattgctgc cagcgttcag gcggtcgccg aggccaccgc
     3421 cggcactgcg caggcgatgc agcaggtcgt gactgtctcg gacaatgccg gcagcatcag
     3481 ccgtgacgtg ctggcgggtg ccgccgatat cggcaatgag gcaaaaaatc tgcgcgagga
     3541 ggtggaccag ttcctggtcg cggtgcgcga cgagaccagc gacgaacgcc gccgctacga
     3601 gcgcgtccgc acgaccggga tcacagcggt ggtacaggcc aagggacggt ccccggcgcg
     3661 catggacctg cggaacgtct cgcgtggcgg ggccgcgctg gcgagcgact ggagatcgcc
     3721 gtccggcact gcgcttgatg tggaactgcc ggacgcaggt gggccggttc cggcccgcgt
     3781 cgtgcggtcg ggcaacggcg agcttgcggt ggtgtttagc tcggaaccac gcgcgttggc
     3841 acggatcgac caggcgctgg cagcactgac cgggacgaag cgagcggctt aaagcaggtc
     3901 gaacgcagcg atcgcgccct gcgctgggcg tcaacgtctg catcagcaga tgacaccgcg
     3961 ccccggacac tctatctgac ctccgcccat gaccccgctc gaccacatcc gcaatttcgc
     4021 gatcatcgcg catatcgacc acggcaagtc caccctggcc gaccgcctga tccagaccac
     4081 tggcgcgctc agcgaccggg agatgaccaa ccaggtcctg gacaacatgg acctggagcg
     4141 ggagcgtggc atcacaatca aggcgcagac tgtccggctg acctacgagg caaagaacgg
     4201 gcaggtctac gagctgaacc tgatggacac gcccggccac gtggacttct cctatgaggt
     4261 ctcccgcagc ctggccgcct gtgaggggag cctgctggtg gtggacgcct cccagggcgt
     4321 tgaggcccag acgctcgcca acgtctacca ggcgatcgag gccaatcacg agatcgtccc
     4381 ggtgctgaac aagatcgacc ttccggctgc cgaacctgcg cgggtgaagc agcagatcga
     4441 ggacgtgatc ggcctggatg ccaccgacgc ggtggagatc agcgccaaga ccggcctcaa
     4501 catcgagggc gtgctggaag ccttggtgca ccgcctgcca ccgcccaagg gtgatgacaa
     4561 cggtcccctg aaggctcttc tggtcgatag ttggtacgac cagtatctcg gcgtggtcat
     4621 cctgatccgc gtcaaggacg ggcgggtgaa gcgcggccag cgcatccgca tgatggccaa
     4681 gggttcagtg caccaggtgg agcaggtggg cgtgttcagc cccaagatga tcccggtgga
     4741 cgacctcggc cccggcgaga tcggctacat caccgccgcc atcaagacag tggccgactg
     4801 ctccgtcggc gacacgatca ccgacgatcg tacgccagcc accgaaccat tgcccggctt
     4861 caagccgtca gtccccgtcg tgtggtgtgg cctctacccg gtagacgccg acgacttcga
     4921 aaaattgcgg gacagcctcg ccaagctccg cctgaacgac gccagcttcc actacgagcc
     4981 ggaaacctct gccgcgctcg ggttcggctt ccgctgcgga ttcctcggcc tgctgcacct
     5041 ggagatcatc caggagcggc tgacccgtga gttcgacctg gacctgatcg ccactgcgcc
     5101 atcagttgtc taccacatcc accgctccaa cggcacgatg gtggagttgc acaacccggt
     5161 ggatatgccg gacggcagcg tgatcgacca catcgaggag ccgtggatcc gcgccaccat
     5221 catggtgccc gacgactacc tcggcgctgt gctgcagctc tgcaacgagc ggcgcggcga
     5281 gcaggtggat ctgacctatg tcggcaaccg ggccatggcg gtctaccggc tgccgctgaa
     5341 cgaggtggtg ttcgacttct acgaccggct gaagtcggtc tcgcgcggct acgcgagctt
     5401 cgactaccag atggacggct acgccgaagg tgacctggtg cgcatctcca tcctggtgaa
     5461 cagcgacccg gtggacgcgt tgtctttcat cgcgcaccgt agcgtggccg aggcccgtgg
     5521 ccggtcgatc tgcgccaagc tgaaggacct tatcccccgc cagctgttca agatcgcgat
     5581 ccaggcggcg attggcggg
//
LOCUS       DATGEM010000067         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_323343, whole genome shotgun sequence.
ACCESSION   DATGEM010000067 DATGEM010000000
VERSION     DATGEM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323343"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VKQ27_01940"
     CDS             <1..559
                     /locus_tag="VKQ27_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLY87723.1"
                     /translation="SHYNGASLWFVNAKEDSPRKLEWKGSHTGIAIHPDGDAVVTAMQ
                     ENALHGWRLSDNQHMRMSGYPAKTAAMSFTRNGKWLATSGADAMVLWPFFGGGPMGKA
                     PIELAGGDGVVCTRVAAHPRDEMVAGGFADGLIVLADVNSSRVLPIAPPGRGPVSALA
                     WSPDGSQLAFGTESGLAAIIDLAKR"
     gene            complement(672..1133)
                     /locus_tag="VKQ27_01945"
     CDS             complement(672..1133)
                     /locus_tag="VKQ27_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HLY87724.1"
                     /translation="MIHYDLKCSEDHTFDGWFKDSAAYERLAKRGLVECPHCGDTKVE
                     RALMRPAVPKKGNAVVPVPQPMPPAPSQDAVAAGPMPAQMRAMLQKMRAEVEKHCDYV
                     GPEFAEEARKMHRGESDKRGIYGETSPEQAEALADEGIEFSRIPWVPRADG"
     gene            complement(1221..1481)
                     /gene="grxC"
                     /locus_tag="VKQ27_01950"
     CDS             complement(1221..1481)
                     /gene="grxC"
                     /locus_tag="VKQ27_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HLY87725.1"
                     /translation="MPEIEVYTQPWCPFCARAMHLLSGKGATFREIDAPHGSPEREES
                     IRRSGGRTTVPQIFIGGQHIGGCDDLVALDRAGKLDPLLRAQ"
     gene            complement(1731..>2015)
                     /locus_tag="VKQ27_01955"
     CDS             complement(1731..>2015)
                     /locus_tag="VKQ27_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87726.1"
                     /translation="LLITGLARSGTSILAALLEAAGIWLGNHLYEPINEDAKITQMLQ
                     ARGFTRLDTLIETQNAKTPIWGFKMPDLHLYMQPDELRRFRKPAPDRNLP"
BASE COUNT          337 a          606 c          684 g          388 t
ORIGIN      
        1 gtcgcattac aacggcgcgt cgctttggtt cgtgaatgcc aaggaggaca gcccgcgcaa
       61 gctggaatgg aagggaagtc ataccggcat cgctatccat cccgatggcg atgccgtggt
      121 caccgcgatg caggagaacg cgctgcatgg ctggcggctg tccgacaatc agcacatgcg
      181 aatgagcgga tacccggcga agaccgccgc gatgtcgttc acccggaacg gcaagtggct
      241 ggcgacaagc ggtgccgatg cgatggtgct gtggccgttc ttcggcggcg ggccaatggg
      301 gaaggcaccg atcgaactgg cgggtggcga cggcgtcgtg tgcacgcgcg ttgccgcgca
      361 tccgcgcgac gagatggtgg ctggggggtt cgctgacggg ctgattgtgc tggccgacgt
      421 caactcatct cgggtgctac caatagcgcc gccaggccgg gggccggtgt ctgcgcttgc
      481 ctggagccca gatggcagcc agctcgcctt tggaacagag agcggcttgg cggcgatcat
      541 cgatctggcg aaacgctgaa cgtcgtggcc gagctcggcc gggtcgtatc ctgcagtcgg
      601 ctcactgatg cgacttgtgg ctgatgggcg acttgatcct cgggtcaagc ccgaggccat
      661 gacgacggga gttacccgtc tgcccgcggc acccagggga tgcgggagaa ctcgatgcct
      721 tcgtcggcta gggcctccgc ctgttcaggg ctggtttcgc catagatgcc ccgcttgtcg
      781 ctctcgccgc ggtgcatctt ccgggcttcc tcggcgaact caggcccgac gtaatcgcag
      841 tgcttttcca cctcggcccg cattttttgc agcatggcgc gcatctgtgc cggcatcggg
      901 cctgcggcga cggcgtcctg ggacggagct ggcggcatcg gctgcgggac cggtaccact
      961 gcgttgccct tcttgggtac ggcgggacgc atcagggcgc gctcaacctt ggtgtcgcca
     1021 cagtgggggc actccaccag gccgcgcttt gccagccgct cataagccgc gctgtccttg
     1081 aaccagccgt cgaacgtgtg gtcctcgctg cacttcaaat cgtagtggat catctgtgaa
     1141 ccggatttct cgcgttgatc agatggcgga ttttggcact ccggacaagg gagtgccaac
     1201 aatttatggc agctctattg ctattgcgcc ctcaagagcg gatcgagctt accggcccga
     1261 tcgagtgcga ccaggtcatc gcagccgccg atatgctggc cgccgatgaa gatctgcggc
     1321 accgtggttc ggccaccaga gcggcggatt gattcctcgc gctccggtga gccgtgaggc
     1381 gcatctatct cacggaacgt ggcgcccttg ccgctgagca gatgcatggc gcgggcgcag
     1441 aacgggcacc agggctgggt gtagacttca atctcgggca tgcctcactc cttcgggcag
     1501 cataacgtcg ggcgtcatcg ctgttcgcgc aaggccttga gcgccgcatc ggtgtcaggt
     1561 ttccgatgtg gcgcgctcgg ctgacccgcc tgttgccaga gatgcccttc ggggtggaca
     1621 catactggct ggacatgctg aagcaggccg gcgcgcagcg cctcgtccag atcgatgccg
     1681 aaaaaaggcg agcacgttgt caatgaatgc gcccggcgac cgcaaccggg tcacggaaga
     1741 ttgcgatcag gtgcgggctt gcggaaccgc cgcagttcat cgggctgcat atacagatgc
     1801 aagtccggca tcttgaaccc ccagatcggg gtcttggcgt tctgcgtttc gatcagtgtg
     1861 tccaggcgcg tgaagccgcg cgcctggagc atctgggtga tcttggcgtc ctcgttgatc
     1921 ggctcataga gatggttgcc gagccagata ccggccgctt ccagcagggc tgcgagaatg
     1981 ctggtaccgg accgggccag gccggtgatc agcag
//
LOCUS       DATGEM010000068         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1005556, whole genome shotgun sequence.
ACCESSION   DATGEM010000068 DATGEM010000000
VERSION     DATGEM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005556"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /locus_tag="VKQ27_01960"
     CDS             complement(<1..2246)
                     /locus_tag="VKQ27_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY87727.1"
                     /translation="MRLIASAVACAALVLPATASAADRVAFAVPPGPLRAAVIAIGDQ
                     ARVTIGLTDPTLGALPVRGVYGRMSVAAALSRLLAGTPARFVQVDEQAFQVIRRPEGP
                     HPRFPPSPTEPAPEAAIVVTGSKRSASIDAYAGSVALVDTGDLRPGRLAHGSDALVEQ
                     LSVLSSTHLGPGRNKLFIRGVADSSFSGPTQATVGQYLGDVRLNYSAPDPDLALYDIA
                     SVEVLEGPQGTLYGAGSLGGVVRLIPVAPDLTHTTFSIAAGGSTTAHGAPGGDASAIL
                     NIPLVTGRLGLRVVSYGGVDGGYIDTVTRDRSDINRTTTHGARATLRFEPAPSWTVDI
                     GGVIQNIDTRDGQYSERGFPSLSRDSVIAQPFDNDYSLASLVVLHEMGATTLVSATSA
                     VIHDISSTYDASITPAQPRRYREEDNILLLTNETRLSHHAANGGGWVIGAELLRSSDR
                     LARTLGPIGADASISGTSNTVEQASLYGEATLRVAPHWFVTGGVRFELARLVGDVIDR
                     PAGQGDDDQHRHDHSLLPSVGIVWNAIPRLTVYARYQEGYRPGGISVQSSGTQRFEAD
                     SLATWELGVRYGTATDRFTATLAGSYAHWEDIQADLVDPIGLPFTANIGSGRIVGLEI
                     QAHWNPIARLTFDGALFVDDSTLSAPTPAFAGEKDASLPNIADMTARIGTHYAFNIGG
                     RRIELSGSIHYVGRSRLGVGPLLDLHQGRYADTAVGLNVPLGRATLSLDATNLFDARG
                     NIFAFGNP"
     gene            complement(2243..3214)
                     /locus_tag="VKQ27_01965"
     CDS             complement(2243..3214)
                     /locus_tag="VKQ27_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLY87728.1"
                     /translation="MTPAERQRHEQALDWVRRIHDPTFADWNGHADWLEAEPRNLDAF
                     DAASLLIEDATEELERAPAPALAVNDNGIQPQTPSRIGWRGWWRGGAGLGLAVAASIA
                     GIVALPSLLSSGGQPYSIATGPGEHRTIALADGTTIALNGNSQILLDRVSSRRANLDR
                     GEAFFTVVHDAAHPFEVHAGSATFQDVGTAFDMVRDTRGTEVAVREGAVMYDPAGAAL
                     SLDGGQAIRIAANGAVIRNTDVAAIGSWRQGRLLYRDATLPEVAADLARSIGQPVSVD
                     RAVRTRRFSGVIIIDDDHPRMFRRLAAVMNVAILPEKNGWRMASPAR"
     gene            complement(3201..3764)
                     /locus_tag="VKQ27_01970"
     CDS             complement(3201..3764)
                     /locus_tag="VKQ27_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLY87729.1"
                     /translation="MAGGPLAEMFMAHRRALLRYLRARGAGDEAEDLLQELWIKLGAG
                     TEDAAINDLLAYLYRMAHNLMLDRRRSTVRRRLREQAYQDDVREPAGIADPAAGAERT
                     LLARDRLRYIDRALSALGTRADSIFRRHRVEGVPQRQIAEELGITLSAVEKHLQRAYR
                     AVAAAQRSYGEDGQTDPTHKEVQDDPR"
BASE COUNT          804 a         1397 c         1412 g          730 t
ORIGIN      
        1 aagggattac cgaacgcaaa gatgttgccg cgcgcgtcga acaggttggt cgcatcgagc
       61 gagagagtag cgcggccgag ggggacattt agaccgaccg ccgtatccgc gtagcgcccc
      121 tgatgcagat ccagcagcgg cccgacaccg agccgcgatc ggccgacata gtggatcgaa
      181 ccactaagct cgatcctcct gccgccgata ttgaaggcat agtgtgtgcc gatccgcgcc
      241 gtcatgtcgg cgatattggg aagcgaggcg tctttctcgc cagcgaatgc cggcgtgggc
      301 gccgacagcg tgctgtcgtc gacaaacagg gcgccatcga aggtgaggcg cgcgatcggg
      361 ttccaatgcg cctggatttc aaggccaacg atccggcctg aaccgatatt ggcggtgaag
      421 ggcagaccga tggggtcaac cagatccgcc tgtatgtcct cccaatgggc gtaggaaccg
      481 gcgagtgtcg ccgtaaagcg atcggttgca gtgccatagc gcacgccgag ctcccaggtg
      541 gcgaggctat cggcctcgaa ccgctgggtt ccactggact gcaccgatat gccgcccggc
      601 cggtagcctt cctgatagcg cgcatagacc gtgaggcgtg gaatcgcgtt ccacacgatt
      661 ccgacggaag gtaacaggga gtgatcatgc cgatgctggt cgtcgtcgcc ctggccggca
      721 ggacggtcga tcacgtcgcc gaccagccgt gcaagctcga accggacacc gccggtcacg
      781 aaccaatgtg gcgcgacgcg cagcgttgcc tctccgtaga gcgaggcttg ctccacggtg
      841 ttgctggtac ccgaaatact cgcgtcggca ccgatcggcc ccagcgtgcg ggccaaccgg
      901 tcggaactgc gtagcaattc ggcgccgatc acccatccac cgccgtttgc ggcatgatgc
      961 gacagtcggg tttcgttggt gaggaggagg atgttgtcct cttcgcgata acgcctcggc
     1021 tgggcaggcg tgatgctggc gtcataggtc gagctgatgt cgtggatcac cgccgaggtc
     1081 gccgacacga gtgtggttgc gcccatttcg tgaagcacga ctaggctggc gagcgaatag
     1141 tcgttgtcga aaggctgcgc gatcacgctg tcgcgggaga gtgacggaaa cccgcgctcg
     1201 ctatattggc cgtcgcgggt gtcgatattc tggatgaccc caccgatgtc gacagtccaa
     1261 cttggcgcgg gctcaaaacg cagtgtcgcg cgtgcgccgt gcgtcgtggt gcggttgatg
     1321 tcggagcgat cgcgcgtgac ggtgtcgata tagccgccat caacgccgcc atagctgacg
     1381 acacgcagtc cgagccggcc ggtgaccaga gggatattga ggatggcgga cgcatcgccg
     1441 cccggtgcac cgtgcgccgt cgtcgaccca cccgcggcaa tcgaaaacgt tgtatgtgtc
     1501 agatcgggcg cgaccggtat caggcgcaca accccgccga gcgatcccgc accatagagc
     1561 gtcccctgcg gtccttccaa cacctccacg gacgcaatgt cgtagagtgc gaggtctgga
     1621 tcgggggcac tgtagttcag ccgcacgtcg ccgagatact gaccgacggt cgcctgggtg
     1681 gggccgctga agctcgaatc cgcaacgccg cgaatgaaga gcttgttgcg cccgggcccg
     1741 agatgcgtcg aggagagaac agacaactgc tcgacgagcg cgtccgaccc gtgcgcgagc
     1801 cttcccgggc ggagatcgcc ggtatcgaca agagcgacac tgccagcata ggcgtcgatc
     1861 gaggccgagc gcttgctacc ggtgacgacg atggccgcct ccggggcagg ctccgtcggc
     1921 gaggggggaa agcgtggatg cgggccttcg gggcggcgga taacctggaa ggcctgttcg
     1981 tccacctgca caaagcgggc cggtgttccg gccagcaacc gcgacaatgc cgcggcgacg
     2041 ctcatccggc cgtacactcc acgaacggga agtgcgccga gcgtgggatc cgtcaggccg
     2101 atcgtcacgc gcgcctgatc gccgatagcg atgaccgctg cacggagcgg accaggcggc
     2161 acggcaaacg ccacgcgatc ggcagcagaa gcggttgccg gcaagaccag agccgcacag
     2221 gcgacggccg acgcgatgag tctcaccggg caggcgaggc catccgccag ccattcttct
     2281 ccgggaggat cgcgacattc atcaccgccg ccagtcgacg aaacatgcgc ggatgatcgt
     2341 catcgatgat gatgaccccg ctgaaccggc gcgttctgac tgcacgatcc accgacacgg
     2401 gctggccgat actgcgggca agatcggcag cgacttcggg taaggtcgca tcacgataga
     2461 gcagccgccc ctggcgccag cttcctatcg cggcgacatc ggtattccgg atgaccgcac
     2521 cgtttgccgc gatgcggatc gcctggccgc catcgaggct caatgcggca cccgccggat
     2581 cgtacataac cgccccttcg cgaacggcca cctctgtgcc gcgggtgtcg cgtaccatat
     2641 cgaacgcggt gccgacatcc tggaaggttg cgctcccggc atgaacctcg aacgggtgcg
     2701 ccgcatcatg gaccaccgtg aagaacgcct caccccgatc aaggttggcg cgacgcgacg
     2761 agaccctgtc gagcaagatc tggctgttgc cgttcaacgc gatcgtcgtc ccatccgcga
     2821 gcgcgatggt gcggtgttcg ccggggccgg ttgcgatcga ataaggctgg ccgccgctgg
     2881 aaagcaggga agggagggca acgatcccgg caatcgaggc agccacagcc aacccaagcc
     2941 cagctcctcc gcgccaccag ccccgccacc ctattctcga cggggtctgc ggctggatgc
     3001 cattgtcgtt gaccgccaag gctggcgcgg gtgcccgctc cagttcctcg gtggcatcct
     3061 cgatcaggag cgatgccgcg tcgaacgcgt cgagattgcg gggctcggcc tccagccagt
     3121 cggcatgacc gttccagtcg gcgaaagtcg ggtcatggat gcgacgcacc cagtcaagcg
     3181 cctgctcatg gcgctgccgc tcagcggggg tcatcctgca cctccttgtg ggtgggatcg
     3241 gtctgcccat cttcgccata gctccgttgg gccgcagcga cggcgcgata ggcgcgctgg
     3301 agatgcttct ccacagcgct gagggtgatg cccaactcct ctgcgatctg gcgttgcgga
     3361 acgccctcca cccgatgtcg cctgaaaatg gagtccgccc gcgtacccag tgccgacaag
     3421 gctcgatcga tatatcgaag tcggtcccgc gcgagcaggg tccgttctgc tcccgccgcg
     3481 ggatccgcta tgcctgccgg ttcgcgcaca tcgtcctgat aggcctgctc gcgcaaccgg
     3541 cgtcgcacgg tactgcgtcg tcgatcgagc atcagattgt gtgccattcg atatagatag
     3601 gcgagcaggt cgttgatcgc ggcgtcctcg gttcccgctc ccaacttgat ccagagctct
     3661 tgcaatagat cctcagcctc gtcgccggcg ccgcgcgcgc gcagatagcg caacagggca
     3721 cggcgatggg ccatgaacat ctcggcgagc ggtccccccg ccatgctgct cctccagccc
     3781 atattcgaca ggcgcgcagg ataggcgcgg accgcaccac cggtaaagta ggggaaagag
     3841 gtcgccacgg ccatcagaag cgggcgcgca tcacgagcga tgggcaaagg tgaaagggtc
     3901 tgtccaattt ccgtttctcc tcatgcccgt tatccgccgg acatccccga tcccgccggc
     3961 cgtcgctgca agactagacg acggcggtgc cgccgacccg cagccaaatt ttggcggatc
     4021 gtccaactgc ggttccgctc ggcgaccggc gtctcgctta cagaatatcg ggaaagctga
     4081 ggcagctgat gaatggatcg agattgatac gcgcacgatc cagcaatgcc gggagccgga
     4141 cgttcagcga gcgaccttac ggttatcacc tcttgatcgt caggttcctt gtctgggcga
     4201 tgcgccacaa caagggctag cagttgccgc gcttgcacgc ggcgtctgtg ctattccggt
     4261 ggcctgcccg gtatcgccaa atcaggcacg tcgcgcgctc gattccgaca tgaccgtacc
     4321 cgaaaccggt cggaccggcc gcg
//
LOCUS       DATGEM010000069         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1577395, whole genome shotgun sequence.
ACCESSION   DATGEM010000069 DATGEM010000000
VERSION     DATGEM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577395"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VKQ27_01975"
     CDS             <1..1130
                     /locus_tag="VKQ27_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HLY87730.1"
                     /translation="DTLAGVADLQGTIEAIARGGTFNIVGDTFQNDGIVIARNHEQLL
                     VNTAISNGTGITEIGGSAVARFTAAIGVGQTLLFADDTGVLQLTSPSSFGGTLFNFHA
                     GNTIDLADIPATTAMWQAGAGAQPGTLSIFNGTTLLATLAIAGDYAAATFQVNPDNAG
                     GSAITVTGPAPCFAAGTRILTPRGGVAVERLREGDVVLTVTGGRERIQWIGYRDVDCR
                     RHINRERVIPIRIAAHAFGQDRPRRPLLLSPDHSVFVEGVLIPVRFLVNGSTIQQMEA
                     DMVSYFHIELDRHEVVLADGLPAETYLETGGRKAFANAAGAMQLHPEFAPDEARVAMI
                     WRAFGYAPLLGSNGEYERTVRMLTMQAALLGCDLGRSRRLA"
     gene            complement(1340..1546)
                     /locus_tag="VKQ27_01980"
     CDS             complement(1340..1546)
                     /locus_tag="VKQ27_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87731.1"
                     /translation="MPQLTKLLAWVEPQGNDECLVAFVGGAATQRVPAMQICASQEEA
                     RRWVEDEAAEFGLPVEWLANPPQR"
     gene            complement(2108..2184)
                     /locus_tag="VKQ27_01985"
     tRNA            complement(2108..2184)
                     /locus_tag="VKQ27_01985"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2148..2150),aa:Pro,seq:cgg)
     gene            complement(2233..2814)
                     /locus_tag="VKQ27_01990"
     CDS             complement(2233..2814)
                     /locus_tag="VKQ27_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLY87732.1"
                     /translation="MPHIHSVAVFCGSRPGNDPAYRAAARSLGLGLAEAGIRLVYGGG
                     RIGLMGVVADAVLEAGGEVLGVIPEFLMRREVAHDGVRQMEITSSMHSRKQRMFEAAD
                     AFVTLPGGLGTLDETIEIITWRQLHLHDKPILLCDVAGSATPLAAVLEAAVTNGFAGP
                     DSRDLYEIVDGVPGVLRRLRSLRTSAQGEPARL"
     gene            complement(2972..>3699)
                     /locus_tag="VKQ27_01995"
     CDS             complement(2972..>3699)
                     /locus_tag="VKQ27_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY87733.1"
                     /translation="ERLKRLQDPAVRATILADRPSDELVNRLSQFRMRITTGWDRMFV
                     MGDPPDYEPEASKSIGAIARRSNHTPDEVAYDYLAGGLDKFLFFPIVGYNEDNHDIIR
                     TMLTSPETVLGLSDGGAHCSSISDASLPSWMLTHWGRDRKRGPGLPLETIVRRQTSET
                     ADFFGFADRGRLAPGLKADVNVIDFERLRLHVPEIRYDLPAGGRRLVQRVDGYVATLV
                     AGTPVFEHGQYTGATPGKLVRAR"
BASE COUNT          674 a         1135 c         1239 g          651 t
ORIGIN      
        1 tggatacact ggccggcgtc gctgatctgc aaggcaccat cgaggcgatc gccaggggcg
       61 gcacgttcaa catcgtcggc gacaccttcc agaatgatgg gatagtgatc gcccggaacc
      121 acgagcagct cctggtgaac acggcgatca gcaacggcac cgggataact gaaatcggcg
      181 gcagcgcagt cgcgcgcttc accgccgcaa ttggcgtggg ccagacgctc ttgtttgccg
      241 acgacacagg ggtgctgcag ctcaccagtc cgagcagctt cggcggaaca ctgttcaact
      301 tccatgccgg caatacgatc gacctcgccg atatccccgc caccacggca atgtggcagg
      361 cgggcgcggg tgcgcagcca ggaacactgt cgatcttcaa tggcacgacg ttgctggcga
      421 cgctggcgat cgccggggac tatgcggctg caaccttcca ggtcaatccg gacaacgccg
      481 gcggttcggc gatcaccgtc acgggtccgg cgccatgctt cgcggcaggg acacgcatcc
      541 tgacgcctcg gggtggcgtt gcagtggagc ggctgcgcga gggcgatgtg gtgttgaccg
      601 tcacgggtgg gcgggaacga atccagtgga tcggttatcg ggatgtcgat tgccgtcgtc
      661 acatcaatcg cgagcgggtg ataccgatac gtattgcggc ccacgcgttc gggcaggatc
      721 ggccacggcg gccgctgctg ctttcgcccg atcattctgt tttcgttgaa ggcgtactga
      781 tcccggtgag gtttctggtg aacggcagca ccattcagca gatggaggcc gacatggtga
      841 gctatttcca tatcgaactg gatcggcatg aggtggtgct ggctgatggg ctgccggcgg
      901 agacctacct ggaaacgggc gggcggaaag cttttgccaa tgccgccggg gcgatgcagc
      961 tgcatccgga attcgcaccg gatgaggcgc gcgtggcgat gatctggcgg gcgttcggat
     1021 atgcgccgct gctggggagt aatggagagt atgagcgcac cgtgcggatg ctgacgatgc
     1081 aggcggctct gctgggttgc gatctcgggc ggagccggcg tcttgcgtga aagtcgtggg
     1141 ccgcgcccaa gtccggtctg atgggaacat cgctacgacc ggtggcggat agataataaa
     1201 ggccgtcgct accgcaacgg cactcgtgcg aagtgctgaa ggtcctttgg atgagagacc
     1261 gcagcgtctt cggtgcaccg ggcgccgatg acctgatggt caccaacagc ctaaacagcg
     1321 tcggtaccgc ggccggctat catcgctgcg gtgggttggc cagccattca accggcagac
     1381 cgaattctgc agcttcatcc tcgacccatc gccgtgcttc ctcctgagag gcgcagattt
     1441 gcattgccgg cacgcgctga gttgctgctc cgccgacgaa cgcgaccagg cattcgtcgt
     1501 taccctgtgg ctcgacccat gcgagtaatt tggtgagctg tggcattgtc acctcgctgc
     1561 gacgaatcat taacggagta cagacatctc agctttttgt cgagtagcac catgaacatg
     1621 gctgaaggcc tcgaaccggc cgtgcggcag ccgctaacgc cttcccacgt ggcgaacgaa
     1681 cggcgatcct cgcgcttggg tcacgcgaaa tcgattggcc gcaaattctg cgagtgcaca
     1741 acaatgatgg catggagcaa ttgccgcaca tcagcccgcc gcaatggagg ctgtatgcca
     1801 gtgaggcgag agtacgggca cagtcgatgc attatccgcc tgcccgtgag acgatggaac
     1861 tcatagcaaa agctgccgag aatatggctg cgaccctgga gcggctggag cgctcgcgct
     1921 taatgcttct ggtcagcgac cgacacagga agacggtctc gcagcctggt cggtgacggc
     1981 cgggcgcgag gggtaacgta cagcgacgat ggcggtggcg ctatcgatac gccggcaatc
     2041 tgccgccctg cgccaatctg actgatccgg cagttacggc gaaggacgcg caccgaattg
     2101 attcgactgg tcggagtgag aggattcgaa cctccggccc ctgcgtcccg aacacagtgc
     2161 tctaccaggc tgagctacac tccggccgag ccgcggcata tagccccggc gctcgcaagc
     2221 gacaaggcgc tgctaaagtc gcgccggctc accctgggca gaagtccgca atgatcgcag
     2281 tcgccgcagc acgccaggca cgccgtcgac gatctcgtac agatcgcgtg agtccggtcc
     2341 ggcgaaccca ttggtaaccg cggcctccag cactgctgcc agaggtgttg ccgaaccggc
     2401 cacgtcgcac agcaggattg gcttgtcgtg caggtgcaat tgccgccagg tgatgatctc
     2461 tatcgtctcg tccagcgtgc cgaggccacc gggaagtgtc acgaaagcat cagccgcctc
     2521 aaacatccgc tgcttgcggc tgtgcatgga gctggtgatc tccatttggc gaacgccgtc
     2581 atgcgcaacc tcgcggcgca tcaggaactc ggggatgacg cccaggacct cgccgccggc
     2641 ttccagaacc gcatcggcga caacgcccat cagaccaatg cggccgccgc cgtagacaag
     2701 ccggatgccg gcctccgcca gccccaggcc cagcgatcgg gcggcagcgc ggtaagctgg
     2761 atcgttccct gggcgcgagc cgcagaagac ggcaacggaa tgaatgtgtg gcatgaacac
     2821 catgtggccg ggcggccccg attctgccac tggtttcgcc gacaggccgg gatttttgat
     2881 ggcggcgagg cgtccggcag ccgggccggc gaggcgacgg gagcgccctc aggtaaggcg
     2941 cgcccctcgg ttactctgcc gccgcgtcgg gttatcttgc gcggaccagc ttgccggggg
     3001 tggcaccggt gtactggcca tgctcgaaga ccggagtgcc ggcgacgagc gtggcgacat
     3061 agccgtccac gcgctgcacc aggcgccgac cgccagccgg caggtcgtag cggatttccg
     3121 gcacatgcag gcgcaggcgc tcgaagtcga tcacgttgac gtccgccttc agccccggtg
     3181 cgaggcgccc tcggtcggca aagccgaaga aatccgcggt ctcgcttgtc tggcgcctga
     3241 cgatcgtttc cagcggcagg ccgggtccgc gcttccggtc gcggccccag tgggtcagca
     3301 tccagctcgg caggctggcg tcgctgatcg aggagcagtg cgcgccgcca tccgacagac
     3361 cgagcaccgt ttccggcgag gtcagcatgg tgcggatgat gtcgtggtta tcctcgttgt
     3421 agccgacgat gggaaagaac aggaatttgt cgagaccgcc ggcgagatag tcgtaggcca
     3481 cctcgtccgg cgtgtggttc gagcggcggg cgatggcgcc gatgctcttg ctggcttccg
     3541 gctcgtagtc cggtggatcg cccatgacga acatgcggtc ccacccggtc gtgatgcgca
     3601 tccggaactg cgacaaccgg ttcaccagtt cgtcggaggg cctgtcggcg aggatggtgg
     3661 cgcgcaccgc cgggtcctgg aggcgcttca ggcgttcgg
//
LOCUS       DATGEM010000070         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1346171, whole genome shotgun sequence.
ACCESSION   DATGEM010000070 DATGEM010000000
VERSION     DATGEM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346171"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VKQ27_02000"
     CDS             <1..529
                     /locus_tag="VKQ27_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87734.1"
                     /translation="PPGLLRPEPHSEGLRDRIWWGSGSNATAVWAAKLGMNLQASTLK
                     SDETGEPFHIQQAAQIRAFRKAWNKAGHTREPRVSVSRSIFALVDDRDRAYFGHGGDD
                     DDSFGFIDPARRSIFGRNYAAEPDKLVEQLRQDEAIAEADTLLLTVPNQLGVDYNAHV
                     LEAILTHVAPALGWR"
     gene            complement(564..1526)
                     /locus_tag="VKQ27_02005"
     CDS             complement(564..1526)
                     /locus_tag="VKQ27_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLY87735.1"
                     /translation="MTRRLKPLDPSVSPRFGDVATLLRAPRVAVSGGIDIAMVGVPFD
                     LGTNYRTGARGGPAAVREASRTMRLVHPTSKIAPFELCEVADVGDVAINALDLNASLA
                     GTEAFFRQLHAMRITPIAVGGDHTIPLPILRVIAHERPVGVVQFDAHPDTLDVLMGTR
                     INHATTFRRAVEEGLIDPKRTVQVGLRGSQFTADDASWGRAAGMRIITMDEFEQLGRA
                     QVIEEIGRVIGDGPTYVSFDIDGLDASHAMGTGVPEVGGYSVRDAQVMLQSLRGRHLV
                     GADLCEVAPMYDPTGQTALNAANLLFEMLCVVADSVVARARSSH"
     gene            1705..>2474
                     /locus_tag="VKQ27_02010"
     CDS             1705..>2474
                     /locus_tag="VKQ27_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY87736.1"
                     /translation="MLADQYGLPLSTASSIARSAYLEGCAAKLTMFPGAIPAFDRAIA
                     ADPTFALSHAARAHALLERGDAESARTALAVATALADNLPPREASHIAYFAHLIAGDT
                     EAALAALPTHLASWPRDVMVLATTAFTNGLIGSSGRPNQKRTLLALLESLAPHYGSDD
                     WWFTAHHGMALSENGQHAAARPLIERSLAQNPANPWAAHATAHLVYEEGDAPAARSFL
                     TNWLTTYPRDGALYSHLCWHLALAHLEAGDILTANSLFR"
BASE COUNT          429 a          922 c          694 g          429 t
ORIGIN      
        1 cccgcccggc ctgctgcgcc cggagccgca ttccgagggg ctgcgcgatc gaatctggtg
       61 gggctccggc tccaacgcga ctgcggtttg ggcagcaaag ctcggaatga acctgcaagc
      121 ttcgacgctc aagagcgacg agaccggcga gcctttccac attcagcagg cagctcaaat
      181 ccgggcgttc cgcaaggcct ggaacaaggc gggccacacg cgagagccaa gggtctcagt
      241 cagccgcagc atcttcgcgc tggtcgacga cagggaccgc gcctatttcg gacatggtgg
      301 tgacgatgat gactcgtttg gcttcatcga cccggccaga cgttcgatct tcgggcggaa
      361 ctatgcggct gagccggaca agcttgtcga acagctcagg caggacgagg cgatcgccga
      421 ggccgacaca ctgcttctga cagtccccaa ccagcttggt gtggactaca acgcacatgt
      481 cctcgaggcg atcctgaccc acgtcgcgcc ggcgctcggt tggcgttgac gccgcaacag
      541 tgcgtccagg acatcccgtt ccctcagtgg ctgctacgcg cccgagccac cacgctgtcc
      601 gctaccacgc atagcatctc gaaaagcagg ttcgccgcgt tcagtgctgt ctggccggtg
      661 ggatcgtaca tcggagccac ctcgcacaga tccgcgccaa cgagatgccg cccgcgcagg
      721 ctctggagca tcacctgcgc gtcgcgcacc gaatagccgc caacttccgg cacaccggtg
      781 cccatagcgt gcgaggcatc caggccatcg atgtcgaagc tgacatacgt ggggccatcg
      841 ccgatcacgc ggccgatctc ctcgatcact tgcgcacggc cgagttgctc gaactcgtcc
      901 atggtgatga tccgcatccc cgctgcgcgt ccccagctcg catcgtcggc agtgaactga
      961 ctgccacgca gccccacctg cactgttcgc ttcggatcga tcaggccttc ctccacggcg
     1021 cggcggaacg tcgtggcatg attgatgcgc gtgcccatca gtacgtccaa cgtgtccgga
     1081 tgtgcgtcga attgcaccac tcccaccggc ctctcgtgcg cgatcacgcg caggatcggc
     1141 aacgggatgg tatgatcacc acccaccgca atcggcgtaa tccgcatcgc gtgcagttgc
     1201 cggaagaacg cctcggttcc ggcaaggctc gcattcaggt ccagggcgtt aatcgcgacg
     1261 tcaccgacat cggcgacctc gcacaattcg aacggcgcaa tcttgctggt cgggtgcacc
     1321 agccgcatgg tgcgcgacgc ctcgcggacc gccgcaggcc cgccgcgtgc gccggtgcga
     1381 taattggtgc cgagatcgaa cggcacgccg accatggcaa tatcgatccc gccgcttact
     1441 gccacgcgtg gcgctcgcag caacgtcgcc acatcaccga agcgaggtga aacggacggg
     1501 tccaacggtt tcaggcgacg agtcatggcg aggctccgaa ggttatctgt ttccgtcaat
     1561 cgacgttgtc tttcctgccc atcttgagat cggcatagcc gcccgtcgag atttcgactt
     1621 tgatcggcat cctcgggtcc cctctgcgga ctgcttctgg atggattgag acacccatgc
     1681 gcctccccgc taatctcccg atccatgctc gctgaccaat acggcctccc cctctccacg
     1741 gcctcttcta tcgcccgcag cgcctacctc gaaggctgtg ccgccaagct caccatgttc
     1801 cccggcgcca tcccagcctt tgaccgcgcc attgccgcgg atccaacctt cgccctctca
     1861 cacgctgctc gcgcccacgc gctgctggaa cggggcgatg ccgaatcggc ccgaaccgcc
     1921 ctcgccgtgg ccaccgccct tgccgacaac ctcccacccc gcgaggccag ccacatcgcc
     1981 tatttcgcgc acctcatcgc cggcgacacc gaggccgcgc tcgccgccct ccccacccat
     2041 ctcgccagct ggccgcgcga tgtgatggtg ctagccacca ctgcctttac caatggcctg
     2101 atcggctcct ccggccgccc caatcagaag cgcaccctgc tcgcgctgct ggaaagcctt
     2161 gctcctcatt acggcagcga tgactggtgg ttcactgccc accacggcat ggcgctgtcg
     2221 gagaacggcc agcacgccgc cgcccgtccc ttgatcgagc gctccctcgc ccagaacccc
     2281 gccaacccct gggcggctca cgccaccgcc catctggtct acgaggaagg cgacgcgccc
     2341 gccgcccgtt ctttcctcac caactggctc accacctacc cgcgggacgg cgccctctac
     2401 agccacctct gttggcacct ggcgctcgcc cacctcgagg cgggcgacat cctcaccgcc
     2461 aacagccttt tccg
//
LOCUS       DATGEM010000071        76786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_321962, whole genome shotgun sequence.
ACCESSION   DATGEM010000071 DATGEM010000000
VERSION     DATGEM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 76786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76786
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321962"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..748
                     /locus_tag="VKQ27_02015"
     CDS             14..748
                     /locus_tag="VKQ27_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87737.1"
                     /translation="MREISERSGGQVSALLANVRDPDSIEAMMTTIWADGPLTGLVNN
                     AAANFLAPTETLSPRGYEAVRSTVMDGSFYTSLACGKRWIAAGLPGVIIGNLVTWVWT
                     GSAYVVPAAMAKAAVHAMTMSLAVEWGPKNIRVNAIAPGPFPTEGAWDKLNPLAETGV
                     GATQADDVPLRRFGKMDELRNLLIFLMSDGCSYITGDTIAIDGGHHLAAPSTFAGLSK
                     LSPADWQRAREAIQASVQKEKQARSI"
     gene            801..1601
                     /locus_tag="VKQ27_02020"
     CDS             801..1601
                     /locus_tag="VKQ27_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87738.1"
                     /translation="MGLATPTKSFDCTIEDGLAHVVLNQPDRGNPIDGEFCREFGLCI
                     AELSERSDVRAMLLSGRGRLFSVGGDLMSLVKQGDALPRTIKAWTGDLHVAIARMVRM
                     RAPVVASVHGNVAGGSVSLMAAADLVVIAESARISAAFSKIGFSPDSGSTTTITRRIG
                     VARARRLFLLGETLDARTALSLGLVDFVVPDGDVQSEAARIAKELAAGPTQAFAAIKR
                     LFAETSDRSFESQLEEEAETLATISRTADAREGVKAFVEKRKPAFVGE"
     gene            1714..3339
                     /locus_tag="VKQ27_02025"
     CDS             1714..3339
                     /locus_tag="VKQ27_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87739.1"
                     /translation="MTVAAARNDASYANFREAGYWLDKTVDQLLTEAVARTPDKVAIV
                     ADRADREQPPRFTYKELEKLADRAASSLVRLGVGRGDVVTVQLPNWWEFVVTAFACSK
                     IGAVMNPVMPILRERELLYILNFCQAKVFIVPKVYRGFDYAAMAQGMRADLPHLKHVI
                     FADGDGETSFDRMLLASEADRLPPGLRPDDMAVLMFTSGTTGEPKGVMHTSNSLIACC
                     KALSGRFGLDSSDVLLVASPVGHMTGYAAIVLLSVYLGGTMILQDIWEAKRGVGLMAR
                     EGVTYTAASTPFLSDICDAVKAGAPQPKSLRSFLCGGAPIPSVLIDRAADELGLKVCS
                     LWGMTEVLSGTLTEPARAAEKSAGTDGRSLEGMEVRIVDTEGRALPAGESGRLLVRGA
                     QMFKGYYKRPELPTFDSEGWFDSGDLAYMDKDGYIRISGRVKDILIRGGENVPVVEIE
                     NLLYKHPAVSAVAVVGFPDSRLGERGCAFIVPRDGSRVDLAAVQAYLGEAKMAKQFWP
                     ERVEVVAELPRTASGKIQKFKLRELATVLADAK"
     gene            complement(3341..4144)
                     /locus_tag="VKQ27_02030"
     CDS             complement(3341..4144)
                     /locus_tag="VKQ27_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLY87740.1"
                     /translation="MTQPSTIIVETRGAIDVLTLNRPAELNAVSPDMISELTAYFSGL
                     HDRPGTRVLILRGNGPQFSAGAELGSDAFAAPGKGRPQRQLKMQQNYSGVIRLMRSCP
                     QPIIGLVHGAACGAGFSFLLACDVRFAAPDARMNAAYIRIGVGGCDMGSGYLLPRLVG
                     LSVTSELLLTGRFLKAERAKAIGLVSDIAPADELLAKGLEFAEEMLRVSPMGLRMTKQ
                     ALNTLIDAPSLDAALMMEDRQQVILLETNDHAEAVAAFRERRNPTYSDQ"
     gene            4277..5152
                     /locus_tag="VKQ27_02035"
     CDS             4277..5152
                     /locus_tag="VKQ27_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87741.1"
                     /translation="MPYQSVFRSDLFKGQTIIVTGGGSGIGRCTAHELAALGANVAIL
                     GRTLDKLVEVQHEIEEDGGRAMSLACDIRDEAMVIGTIDAVLARYGRIDGLVNNAGGQ
                     FRAPLKSISTKGFEAVVRNNLTGGFIFMREVYNRWMEANGGSIVNIIADIWHGWPEFG
                     HSAAARGGMLTLTETAACEWSSSGVRVNAVAPGGIVSSGFDTYTPEMQKKLHDFTERV
                     PLQRFGTEAEISAAITYLLSPAAAYITGSCIRVDGGTPNARTTWKLEPHSRSQPFEGF
                     HRTKLPEVMKRKASA"
     gene            complement(5157..6170)
                     /locus_tag="VKQ27_02040"
     CDS             complement(5157..6170)
                     /locus_tag="VKQ27_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLY87742.1"
                     /translation="MSVRENRQVRLTSRPNGIPQAEHFSLVTAPVAAPGKGEMLVENR
                     YLSVDPAQRGWANDEGNYSAPVPLNTPMRALAVGEVVESNTPEFRAGEFVYGWFGWQR
                     YCVTTPDTVLRRVTPSALPLSANLSVLGMNGLTAYLAFHGLCDPKPGEHVLVSTAAGS
                     VGSFVGQLAQIAGCRAVGLTSSADKIAQAKARYGYQDMINYREAADLGAAIRAACPEG
                     NDIFFDNTGGAIADAAIRTMRLRGRIIQCGTAANASWTPVPTGPRPEREILTRRLRWS
                     GFIIFDHLAEFEAAAMRLAELALAGRIVHDEEILPGLEHAPGAIAQLYRGENHGKLII
                     AVD"
     gene            6299..6751
                     /locus_tag="VKQ27_02045"
     CDS             6299..6751
                     /locus_tag="VKQ27_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLY87743.1"
                     /translation="MAGLYFEQFSVGQTFVHEIRRTVTDMDNILFSSLTYNPAAVHID
                     HEYAKGTEFGKPLMNSIFTLGLIIGLSVQDTTLGTTVGNLGMEDTKFPRPVFAGDTLR
                     AETKVIAVRESKSRPTQGIVTFEHRGFNQRDEEVVYCRRSGLMMRRPT"
     gene            6748..7632
                     /locus_tag="VKQ27_02050"
     CDS             6748..7632
                     /locus_tag="VKQ27_02050"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLY87744.1"
                     /translation="MKLRSLLFVPADSERKYIKASGVGADALILDLEDSVAPGRKAFA
                     RDAVKGLLGGGPRNWSFLVRINPFGTGLTLEDLAAVVRPGLDGILIPKVNGIEDVNLV
                     SHYVDVLEVATGISPGHVKLLVVATETPAAMIGFNGYARKNERLVAMTWGAEDLSAAL
                     GALTNKEADGAWTFPYQVARAQCLFAAGAAGVAALDTLYADFKDQDGLAESCRLARRD
                     GFVGRVAIHPDQVATINTCFTPSDADLAHARRVVAAFAAAPDVGTVGIDGKMYDIPHL
                     VAARRTLASVGEEHSNGQ"
     gene            7622..8809
                     /locus_tag="VKQ27_02055"
     CDS             7622..8809
                     /locus_tag="VKQ27_02055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87745.1"
                     /translation="MDNRSFSNQLDEPEDHAAIREGVRAVVTRFDDEYWLARDDDGEF
                     PREFHRAMAEAGWLGITMPEEYGGSGLGVTEAAIMMHEVASHGGGMTSASAVHINLFG
                     PHPIVVKGTDEQKRRWVPRLVSGEDQCCFGFTEPDAGLNTTRIKTFAEKVPGGYRVHG
                     QKVWTSTAQVANKIMLLTRTTKFEDCKRPTDGITIFYTDLDRSKIEVRRIPKMGRKAV
                     DSNAIFIDGLFIPEADRIGEEGKGFSYILHSLNPERILIAVEAIGIGQDALRRATQYA
                     KERVVFDRPIGQNQGIQHPLAEKWMYLEAAWLMAMRAAWLYDTNKPCGAEANSAKFLG
                     ARAGHDAAWQAIMTHGGFGYAKEYHVERLFREVSITRLAPITEQLILSFIAEKVLDLP
                     KSY"
     gene            complement(8828..9973)
                     /locus_tag="VKQ27_02060"
     CDS             complement(8828..9973)
                     /locus_tag="VKQ27_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87746.1"
                     /translation="MLPNRPVFDEEHALLRETVRRFAASKIAPHFQEWEKAGIIDRAL
                     WPAAGQAGLLCPQVSEEYGGIGGDFRHNAVVIEELAYSGFAGPATDFSVHNDVCCGYL
                     LSYGTEEQKKKWLPRMIAGETVCAIAITEPGTGSDLQGVRTRAVREGNEYVISGQKTF
                     ISNGQMCDLVIAVTRTNPDGGSRGMSLILVETTRPGFRHGRNLDKLGHLSSDTSELFF
                     DQIRVPVTNLLGSEGGAMAALMSELPQERLTIALHSIASAQKAFDITKAYVLERKAFG
                     QAIGTYQNTRFKLADLKSELQVGWAYVDQCLSQHIRGELTTYAASTAKLWVTEMHGRL
                     VDQCLQFFGGYGFMREYEICRLFADARVLRIYGGTSEIMRELISRNL"
     gene            complement(9997..10926)
                     /locus_tag="VKQ27_02065"
     CDS             complement(9997..10926)
                     /locus_tag="VKQ27_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLY87747.1"
                     /translation="MAPHKVIISCAVTGSIHTPTMSPHLPVTPAEIAESALGAAAAGA
                     AIVHLHARNPVDGRPDQSPEAFEPFLRVIKQSSNVVVNLTTGGSPYMTVEERVRPAAT
                     WKPEVASLNMGSMNFGLFPMLKRYKSFKYDWEPQMLEGSHDLVFRNSFKDIRYALETL
                     NSSGARYEFECYDTSHLYNLHYFWTEGLVKAPLFIQTCFGLLGGIGSHPDDVMHMKRT
                     ADRLFGDNYRWSVLGVGRAQMSVAAMAAAMGGNVRVGLEDSLWIGAGRLAETNAAQVT
                     QVRKIIEGLGLEIASPDEAREILQLKGGDKVAF"
     gene            complement(10933..11727)
                     /locus_tag="VKQ27_02070"
     CDS             complement(10933..11727)
                     /locus_tag="VKQ27_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87748.1"
                     /translation="MTDPKQFEDVIYEIRDRAAWIIINRPKVYNAFRARTLDELIQAF
                     QLAANDRNVASIVLTGAGEKAFCTGGDQSAHEGQYDGRGVVGLPIDEIQGLIRDVPKP
                     VIARVNGFAIGGGNVLATLCDLTIAADHAQFGQVGPKVGSVDAGWGTAFLARHVGDKK
                     AREIWYLNERYTAEQAREMGLVNKVVPMAQLDAAVKDWTDKLAQRSPTAIALAKRSFN
                     ADSDNIRGISNFALHAVKLFYDTAESKEGVAAFNEKRDPDFHKFAR"
     gene            complement(11746..12504)
                     /locus_tag="VKQ27_02075"
     CDS             complement(11746..12504)
                     /locus_tag="VKQ27_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87749.1"
                     /translation="MKGLSGKVAVVTGGGQGIGRGLTLRLAEEGCKIAIFDINPQGGE
                     ETAKLAPQAVIKTYAVDVGDADSVNAAVARVEAELGPIWLLVNNAGWDRPMPFLKTDR
                     ELWDKIIRINLYGPLNTHKAIAPLMAERGGGRIVNIASDAARVGTSDEAVYSACKGGL
                     ISFTKSLARELARKNVLLNAVCPGPTNTPMMASVLGEGEHAVKWKDAMLRGIPLRRMG
                     EPDDYGGIVAMLASDDGKFITGQTISVSGGMNMI"
     gene            complement(12577..13758)
                     /locus_tag="VKQ27_02080"
     CDS             complement(12577..13758)
                     /locus_tag="VKQ27_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87750.1"
                     /translation="MIERTIFREEHEIFRQTVRRFVEREIVPFHAKWEEDGIVPRDLW
                     LKAGAAGLLCCTVPEEYGGMGLDYLFDVVVFEELWRAGASGPGFLIHTDLVATYLLSF
                     GSEAQKRKWLPKMVSGEAIGSLGMTEPHAGSDLKAIRTRATRDGDDFVINGQKVFISN
                     GQLCDFIVLATKTDSQAGAKGVTLFIVESSRDGFKRGRNLEKLGMRAQDTSELFFDNV
                     RIPASNMLGAEGRGFAQMMTKLSQERLAQAIRSATVTETVIDWTLQYTSERSAFGQKL
                     ADFQNTQFKLAELKTKATVARVFTDKCIALFMEGKLDPVDAAMAKMFTSELHCETVDE
                     CLQLFGGWGYMWEYPIARAYADARVVKIAGGSIEIMKTIIARQMYSQRGFDLQKNGSA
                     S"
     gene            13888..15186
                     /locus_tag="VKQ27_02085"
     CDS             13888..15186
                     /locus_tag="VKQ27_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87751.1"
                     /translation="MAVGPELKQFRIELSKERVLHLIFDMPGRSMNVFSNAAIEELGL
                     FADWLKHSDVAGVVIRSGKSSAFCAGADLAELETAYDMIMAAPPGERDRLAFDHFFRL
                     SHGLRRLEAAGKPVAVAIGGLALGGGCEFALAAHHRVIADHPQATFGLPESLVGLLPG
                     GGGTQRLPRLIGIEAALPVLLEGVRLGGQAALAAGLAHELVAPGEEVAAAERWVLSRP
                     QAAQPWDQPTWRVVDAQGVSAAIREKRNKMLAETLGHYPAPSAILDCVEQGLPQDFDT
                     AIRTEMQIFAKLIQRREPRNMIRTLFLGRLDYERLKKKGMNPKIAEAVAAVSRALGVP
                     SERGKELSEAFARAGFPVDQPRKLEFDGHIAGAVYWHDGEPRTWGKELLRARLADAEA
                     AAASWRSQLDESERRAADYVLVTQAGFPAYLGGLFAAGLS"
     gene            15212..16402
                     /locus_tag="VKQ27_02090"
     CDS             15212..16402
                     /locus_tag="VKQ27_02090"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY87752.1"
                     /translation="MSRPLEGIKVLDLSKVLAGPLCAQYLGDLGAEVIKVEAVGQGDE
                     TRGWPPFPAPGLGTVFLSANRNKRSIAINMKSDKGRKLVHELAKSADVAIESFGTGVA
                     ERLGIDAASLCALNDRLVHCSISGFGRTGPLKNSPGYDVILQAFSGIMSMTGDEGGGY
                     IRSPISPIDQMTGVHAFSGILASLFARERSGRGAAIQVSLFDTALGLLGYNLQTFWER
                     GTQPAKCGSSHESLCPYQAFETADGPIMIGVANDNLWRKFCAVAGLSAIVDDPKFRTN
                     AERVKHRAETLHHVQSVLATKTVAYWNAVLNEVGIPCSPINTLAQLLDHPHTRADKLI
                     MHYDHPAAGSLNCVGHPVTFVGAERSPGLPPPMLGQHTDDVLKELGLSAASIADLRRE
                     EIVS"
     gene            complement(16440..16829)
                     /locus_tag="VKQ27_02095"
     CDS             complement(16440..16829)
                     /locus_tag="VKQ27_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY87753.1"
                     /translation="MSEVKKYPAPVTNPETAPFWEAAKQGKFMIKRCTACGEAHYFPR
                     SICPFCFSDKTEWEESSGEATIYTYSLMRKSPTGPYAIGYVTLKEGPSLQTNFVDCDL
                     TALKIGQKVKVVFKPTDGAPLPFFTTI"
     gene            complement(16829..17977)
                     /locus_tag="VKQ27_02100"
     CDS             complement(16829..17977)
                     /locus_tag="VKQ27_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HLY87754.1"
                     /translation="MTIKGKAYIAGIYEHPTRHAPDKSTAQLHAEVAKGAIEDAGLSK
                     DDIDGYFLSGDAPGGAWPMVDYLGLKVRHVDSTDTGGCSYIIHLGHAAEAIAAGKCSI
                     ALITLAGKPRTGPPQVRAPGAEADFETAYGATTHNAYGMCAMRHMHDYGTTSEQLAWI
                     KVAASHHAQYNPHAMLKDVVTVEDVVNSPMISDPLHRLDCCVVTDGGGALIVTSPEIA
                     KSLKKPLVRLIGHGEAMKGPRGGKDLDLTYSAGVWSGPRAFDEAGITPKDIKYASIYD
                     SFTITVLMQLEDLGFCKKGEGGKFVADGNLISGVGKLPFNTDGGGLCSNHPVNRGGMT
                     KIIEAVRQLRGEAHPKVQVKNCDLAIAHGTGGLLGVRHAASTAILERV"
     gene            complement(18056..19252)
                     /locus_tag="VKQ27_02105"
     CDS             complement(18056..19252)
                     /locus_tag="VKQ27_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87755.1"
                     /translation="MRTSILVSASVLFLTGAIFEAGAAEKQYGPGVTDTEIKIGQTVP
                     YSGPASAFSSYGRVMSGYFQMLNEAGGINGRKVNLISLDNAFSPPKAIEQTRKLVDDD
                     GVLAEVGTVGTVPNVAVQKYLNQNKVPQVFISAGGRRFNDPQNFPWTVPFYPPFEMEG
                     TIFGKFILKKMPNAKIAVLYQNDDYGKDYLTGLEAGLGADGTAKIVAEASYELSYPTI
                     DSEILKLKASGADTLVYFTTPKFAAQGIKKANEINWKPVQFLASPVNSIQGVLTPAGL
                     DNVQGAYTTQFAKQASDPAWAEDAEVKDYVAFMKKWAPNDSPNDFIALSGYINAQAIA
                     KGLQRCGDNLTRENLLAQATSFNKERVGMLLPGIELTNSKENYAPYHSLRMAIFDKTS
                     WKLLDE"
     gene            19515..20687
                     /locus_tag="VKQ27_02110"
     CDS             19515..20687
                     /locus_tag="VKQ27_02110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLY87756.1"
                     /translation="MAASGATALAALKIGGRVAFERLMRPKARRADDVPCSPDAITPE
                     WLTAVLCQKVPGAVVTGVVVKPSSAGTHERHQLIISYNEAGRRAGLPGSIFTKSLPSV
                     VTRMIGGFNGTARVEGRFYMQLRPQLEIEAPIGYHAASDRKTFASILLLEDMVATKSA
                     TFCNYRTYVSREMAEDMIGVLAALHARFHGDSELATRYPWVASYPRWFTIGAAKMSLE
                     YYTRKAFDAAARVIPASVLAQRDDVWPAATRALAIHDSEPQGLIHSDVHIGNWYRTGA
                     GKMGLCDWQCLARGHWSRDFAYAVTAALTPDDRRKWEKDLLNRYLDRFAQLTGARPDF
                     DTSFLRYRQQMVHALLMWTITLCHSPLLPAMQSGETTLAMIERISTAMADLDALRS"
     gene            complement(20720..21493)
                     /locus_tag="VKQ27_02115"
     CDS             complement(20720..21493)
                     /locus_tag="VKQ27_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HLY87757.1"
                     /translation="MGDDPLDEDELTYVYEGGNQRAVPSMGVTLGWPPLWIAEPQTGI
                     AWTKALHGEQRLTLHRPLAVSGSIRAEHRVSAVDDKGTARGALLYFDTELFDATRGER
                     IASLRATEFLRGDGGCGSHGMPPGEMIRIQSDAEPSASIDYRTPRQAALLYRLVSRDY
                     MPIHADPAIAREAGFDRPISHGLNTMGLACRAVLKRYASGHPERLRTMAVRFVSPAFP
                     GDTIRIEMFEEGSTIRFRAWAVERRVLVLDRGECALAAR"
     gene            21542..21742
                     /locus_tag="VKQ27_02120"
     CDS             21542..21742
                     /locus_tag="VKQ27_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87758.1"
                     /translation="MDRLEAAREDVFKVHAGAPCDARLPKPRKARPPSPGKIVAVRTK
                     FVKSLERAGRVSRMMSQVRRAD"
     gene            21977..22834
                     /locus_tag="VKQ27_02125"
     CDS             21977..22834
                     /locus_tag="VKQ27_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY87759.1"
                     /translation="MIALLIANSLALASLLIMLSSGLALIYGLRDVINFGHGAIYMLG
                     AYIGYTIALVGGFWLALFLVPILLALLGIVFEYLALRPLQRRTHIEVALVTLGLGIIL
                     GQIIIFFYGGEARSVDAPKILSGSITVLNLSYPVYRLFLIVMGLGSCALLALWLRCTP
                     SGLYVRAVSQDPSVARMMGINADRLSLLVTSLSTAFAGIAGVLAGPYLSIDPGMDITM
                     IVNCLIIVVIGGTGSIGGAIVAALLFGLVQVAGSVFLPNLASLVPYILLMIVLLIAPG
                     GIGRGRVLS"
     gene            22831..23865
                     /locus_tag="VKQ27_02130"
     CDS             22831..23865
                     /locus_tag="VKQ27_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY87760.1"
                     /translation="MSNGTMTGDLQRTATGAPGGQLSRISLVLSGGAIALVAGALVPT
                     YLAGDFVLRLGSEGLLLGMLALSVAFMMNQAGLVALGAASIYGGAGYLFAIGMSDGGL
                     TPAVALCVGFLVIVAYAAVLGALIVRTNPLAFMMLTLAAGEMISHAVLLEGLRDYTGG
                     ADGLVVRMNGSILGIDVAEFADPARFWSLAWIVTVLVGLAFWSLGRSRLGAILRAVRE
                     NEERMRFSGFNTFVPRLVAYVLVNAVAAIAGFLHVLNAGFVSPESLGLSVSTNALVAA
                     LMGGIAGSLGPILGGLIFSFAQDEFGARGLTQLLTGVAIVLFIVVFPRGVVGGVSSAL
                     RRFGSRREMS"
     gene            23867..24565
                     /locus_tag="VKQ27_02135"
     CDS             23867..24565
                     /locus_tag="VKQ27_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY87761.1"
                     /translation="MLKIEKLSVSYGSLRALTGVDLEVGGDGVFHGVIGPNGAGKSTL
                     LDAICGRRRPTSGIVRYRDEDITRRSVTWRRRHGMARSFQRTSIFQDLTIREQLELVA
                     QHLGDDRLDDVVEVMDLGAYLNQRAGRIAYGVQRRVDVALALIGRPRLLLMDEPGAGL
                     SAAETLRLFQHVRDLVRERGIAAIVVEHDVDAVFACCSTVTVLDLGRHLATGTPEAIR
                     ADHRVIAAYLGTAA"
     gene            24562..25257
                     /locus_tag="VKQ27_02140"
     CDS             24562..25257
                     /locus_tag="VKQ27_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87762.1"
                     /translation="MSALRIQSLFAGYGASTVLRGIDLTVEEGESVALVGRNGAGKST
                     LLLSVFGETKIRSGEIWVRGRRIDNLPGYTAAKLGISIAPQGRRILPNLTVKENLLLS
                     RATGRSGYWNLDTIYDLFPVLEERADRLGTMLSGGQQQMLAIGRALMANPSLVFLDEP
                     SEGLSPVLIDGLVETLNRIRGAGTGVLVVEQHLSLVKRATDRFLILAKGEVVGSGAIG
                     VIGTPENQALMAL"
     gene            25289..26506
                     /locus_tag="VKQ27_02145"
     CDS             25289..26506
                     /locus_tag="VKQ27_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87763.1"
                     /translation="MTKSFIDWGSTRLRAATIGAAMALAVTTGALAEDTVKIGVLLID
                     SGPLAGLKDTQVKAVNLAIDRINAAGGAAGRKLEAIFITYPGTPDTAVDGATRAVQKD
                     GALFITGMDTSAVTPALQAKLPALKVLMLEVMANADGLTGKNCSPNYFRVNANDSMIM
                     GTFREFLKDQGIKKWDILAVDYAAGRDSAEKFKGLVTSQGGTVGKTLFSPSGTPDFGA
                     KISELGADPADGLFVTIFGSDAINLAKQQQQFGLFKKYKMVLGNSFVIPQTLPAQGET
                     VLGVYQNIGFVPGFPGAQAEAFVKAYKEKYNGELPPYTSADQYGAIELMAAAIQKANS
                     TDINAVRAALSGLKAETVLGDVEVRAADHQTARRMAISQIAMGADGKPGYQIKKIEPG
                     ADIIPPVDAACKM"
     gene            complement(26594..27613)
                     /locus_tag="VKQ27_02150"
     CDS             complement(26594..27613)
                     /locus_tag="VKQ27_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLY87764.1"
                     /translation="MKAAVLERRGIDGVSWRDFPDPAPAPDESVLKVAASSVNRVDLY
                     MRDNGKGITHTLPQVMGVEAAGTIVEAAPGSGLRPGMKAVLFSEAYCGRCRYCLAGDQ
                     PLCENVKIMGEHRHGGFADYIAMPSNCFFPLPDDADLVGAGALMTGHLTAWRMLFGKR
                     ALQPGESVLIVGIGGGVAVACLQLARMVGARVCVTSSSDEKIAKALALGADGGVNYRH
                     DRVSAAIQEMSNGGVDMVIDSVGEASWSESLRSLRRGGRLVTCGATTGSNPPADLQRV
                     FIRQLEIYGSTGGSISEFRQLLDVFNRGLVKPVIDSSHAMTDVRSALARLASGAQFGK
                     VSLVA"
     gene            27792..29450
                     /locus_tag="VKQ27_02155"
     CDS             27792..29450
                     /locus_tag="VKQ27_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87765.1"
                     /translation="MSETRFDSAAHARAMRASGFWVDKSFDEFLQKTVAATPEKLALM
                     AERADRTEPKRLTYAELGDLISRAAAALRRLGVGPADVVSVQLPNWWEFAVIALAAFR
                     VGAVVNPLMPIFREHELTYMLGFAETKLLVVPKLFRGFDHEAMVRALQPGLPKLKHIV
                     VVDGEGPASFDQALLSGNERLGPPPVGDLGALPADRMAVLMFTSGTTGSPKGVMHCLN
                     TLMGCCIGLAGRFGLDASDTMLVCSPLGHMTGFAAGMLLGLKIGATVIFQDVWEPKRG
                     VSIMAKEGVTYSAGAATFLADMCEAVAAGAAKPAQLRKFLCAGAPIPPALIDRAYREL
                     DLKVCSLWGMTESLASTLTEPERALEKSSKTDGRPIEGVAVKVVRPDGSPAAVGERGL
                     LKVRGAQMFLGYYKREDMEPFDAEGWFDTGDMAYMDDEGYIRIDGRIKDIIIRGGENV
                     PVQDIENLLFRHPSVLTVAIVGYPDPRLGERACAFVVVRPGEKLDLAGLQAYLAEHKV
                     AKQYWPERNEIVAELPKTPAGKVQKYQLREIAKAFSEPTQKASA"
     gene            29447..30208
                     /locus_tag="VKQ27_02160"
     CDS             29447..30208
                     /locus_tag="VKQ27_02160"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY87766.1"
                     /translation="MSLRLKDRVCVVTGAARGIGLSIAERFGEEGGRLACVDVSARRL
                     EPAVGQLKEKGYEARGYEVDVGKRDDVHALFSDIERDFGSPVAVLVNNAVWARFEPLA
                     EIDVGAVERMFAVGVHGLIWTTQAAAPQMERRGGGSIVNLSSVSAFHPAKDSIAYSAL
                     KAGVVGLSRASAIELGAKNIRVNVIAPGMIGTPASIAQFDEATIKARQSEMPAGRFGR
                     PEEIAALAAFLASDDASYIQGAVITADGGWTVPAR"
     gene            30219..31004
                     /locus_tag="VKQ27_02165"
     CDS             30219..31004
                     /locus_tag="VKQ27_02165"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87767.1"
                     /translation="MRRLEGKRAVITGGGSGIGRASAIRFAREGAAVLVVGRAGNAEE
                     TAAAIRSEGGVASALITDASIEENVARIVSHCVSEFGGLEVFFANIGVTGTNTPLLEQ
                     TVEEWSEVYRINTISCFLAVKYAGRHMTAKGYGSIILTSSAASLRANAGAISYSASKA
                     AVNNLTQTAANAFAGTGVRVNAVLPGLVETQLTRKVFEDARARGVESKLGHVTPLKRP
                     GRSEEIAAAAAFLASDDSSYVDGQLLSVDGGVSSTHPYGRIAL"
     gene            31045..32289
                     /locus_tag="VKQ27_02170"
     CDS             31045..32289
                     /locus_tag="VKQ27_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87768.1"
                     /translation="MDFELPQEITAKLAELDAFIEAEIKPLERDNKQFFDHRRENART
                     DWDNDGRPRDEWRALISEMERRADKAGHLRYGLPKSCGGQGASNLAIAAIREHLAAKG
                     LGLHNDLQDESSVVGNFPIIPALADYGTAEQKTYIEGIITGKKHLSFGLTEPGHGSDA
                     TWLETSGVRDGNHWVINGRKRWNSQVARAHANLVFARTSGKPGEAKGITAFIVPTDTP
                     GHKVLYNHWTFNMPSDHAETELLNVRVPDSAILHREGEGLVVAQRFVHENRIRQAAAS
                     AGAARYCIQEAVKYARSRVAFGEPLAKKQAVQFPLVELWAEYQMLRNFIFRTAWQMDR
                     QNPLEISDMVSICNFRANRLCCEAADRAMQVHGGMGYSRALPFEHIYRHHRRYRITEG
                     TEEVQMRRVAQYLFGFSGKIAS"
     gene            32295..33335
                     /locus_tag="VKQ27_02175"
     CDS             32295..33335
                     /locus_tag="VKQ27_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HLY87769.1"
                     /translation="MNVQANDDRLDFDLRRLDAYLKEWLGGSAATRVTRTSGGMSNPT
                     YFVMRGDWRAVLRKQPNNVLMPSAHAIDREFRVLTALQDSAVPTPRPYRYCEDRAVLG
                     TPFYLMEWLVGRVVYDYALPGFTREERAACYRSMCATLAAIHKLDYKNAGLDDYGKPG
                     NYFARQLSRWSKLWEEYRRGDDDNPALDRVVRWLAERVPASETVALCHGDFRMANVIF
                     HPTEPRVTGVLDWELSTLGHPLVDLAFNSQAWRMAPDENGGLLGLPLDELGIPCERDY
                     LDRYYEDSGATERLTVFHQVFAMFRGAVGSAGVAARGDLGNNVLPDAANVGRRLARAY
                     ATRGMALIESEA"
     gene            33332..34018
                     /locus_tag="VKQ27_02180"
     CDS             33332..34018
                     /locus_tag="VKQ27_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HLY87770.1"
                     /translation="MSAAERPLMNVDEAIRLRRSVRGFLPREVPEATLREVFMLAQCA
                     PSNCNVQPWNPHVVSGEKLRQLREALVAAGMRDEPIKPDWPADGKFAGVYRERQVGAA
                     QALYGAMGVARSDAAGRRLAYIRNHAFFDAPHAVFIFMPEPFDTREATDIGMYAQTLM
                     LALTARGIGSCAQGALGLFPDIVREQLEIPGNHKLLFGISFGYEDHSVKANAARVDRA
                     RIDDIVRFHR"
     gene            34054..35205
                     /locus_tag="VKQ27_02185"
     CDS             34054..35205
                     /locus_tag="VKQ27_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY87771.1"
                     /translation="MTGPLQGLRVVEMGGIGPAPFAGALLGDLGADVVRIDRIAKAGV
                     EPELPARFDFYNRNKRSVALDLKRPQAIATVLKLLAQADALIEGFRPSVMERLGLGPE
                     TCQAANPRLVYARMTGWGQEGPLAQEAGHDINYLALTGALHCLGDADRPPPPPLNLVA
                     DLGGGALYLVVGLLSAVMEARQSGQGQIVDVAMIDGVTHLMSAFQAFRQQGSWTARRT
                     DNIVDGGAPFYRTYATRDGKFVAVGAIEPHFYANLLKVLGLNAEDVPDQNDRSAWPRM
                     RERFAGIFAKQTRDAWVAAAMGQDACLAPVLTIDEAPSHPQMKMRNAYAMFDGVRHPE
                     PAPRFSRTRSELHRAPPAPGRDSRAALTDWGIPPDEIDALQAIGAMAGP"
     gene            complement(35235..36449)
                     /locus_tag="VKQ27_02190"
     CDS             complement(35235..36449)
                     /locus_tag="VKQ27_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87772.1"
                     /translation="MNRHFGRALCIIAASATLASTPLHAQKSYGPGASDTEIKLGQST
                     PLSGPASAFGAGAGRAVVGYFDMINEQGGINGRKINFTQLDNAYSAPKAVEQSRKLVE
                     DVGVLAEVGTIGTVPNVAIQKYLNSKQVPQLFITAGGRRFNDPKTFPWTVPLYPDFET
                     EGRVIAKYILNAKPDAKIGVLFQNDDFGRDYVKGLRAGLGAKASQIVAEASYELADPT
                     IDSQIVQLKAAGVDTLIEQSSAKAAAQSIRKVHELNWSPLHIIGGSTASVETVLKPAG
                     LDASKGLVTTQFLKQPGDPAWADDEEMKAYKGFLKKYAPSANPDDYSVLVAYMNVNAL
                     TTVLKKCRDELTRDNLIRQATSLHGTRMPLMLPGVSISTAPGDYTPFKILRIAKFDGT
                     SWAISGDPISAD"
     gene            complement(36484..37623)
                     /locus_tag="VKQ27_02195"
     CDS             complement(36484..37623)
                     /locus_tag="VKQ27_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87773.1"
                     /translation="MSFTEEQIAFRENVRKMATKHVAPIAAEIDETDRFPLELVKLFG
                     EMGLMQLWVPEQYGGPNGNLTMACIAREEISRFSPACASIAALNTMFIMPLLHFGSEE
                     QRKKYLPIIAKGGIVTAIAISEPQAGSDVTAINTRARKDGDCYVLNGRKQWCSYGVAA
                     DYIVVMARTSDSPGADGISAFIVEPKNMAGITFGRHERKMGFRGAPNTPIFFDNVRVP
                     IENLVGEEGKGFRASMRALDLNRPTIGAQSVGLAQGALDVCVAYAKERKQFKKSISEF
                     QGVQFMLADMAMQIEAARALVYECARAGDAGDWKRLNILASMAKCVGSDMAMKVTTDA
                     VQIFGGYGYTMDYPVERMMRDAKLTQIFEGTNQIQRMVIARELLR"
     gene            37635..38570
                     /locus_tag="VKQ27_02200"
     CDS             37635..38570
                     /locus_tag="VKQ27_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLY87774.1"
                     /translation="MGFSLGGQKGASIEWVIHLIIHRMSTKSSPRNSTGSRVASSKRR
                     ERAADHRTAGSPAPVAFRPIRTRRAFEEICDRIREQLALGVLKPGDKLPPERDLAQQL
                     GVSRNVLREALRSLEMAGVLRLQKGVKGGAFVQQGDTSRMNVVMRDMLSLGTISIREL
                     SEARVQVLDLVVRLACVNARQSDFEALQANVERTELFTREGRLLERVECAREFYKLLA
                     VACGNKVIAMITDAVTEIHMRFVYAKVASSGVAMARLGERRRQFLAALRERNATAAAR
                     LMRSHLGAVQKMLEQDPGAMSLQVALAEMKPGMRR"
     gene            38663..39433
                     /locus_tag="VKQ27_02205"
     CDS             38663..39433
                     /locus_tag="VKQ27_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87775.1"
                     /translation="MADKVATVTLNRPQARNAINQKLIRELRTIWDDLADDHAVNVVV
                     LTGAGEFFSVGGDVKAMSERPGGDVLEEGEVHDPMISRRGVTRQLELDKPIIAAINGD
                     AIGLAATHALLCDITVMAEDARIGDTHVSRVGLVAGDGGTVIWPLLVGINKAKEFLIR
                     GTLLKGKEAERIGLVNHVAPRAEVLAKAREIAIELANGPTWAIRWTKLSINQIVKDRV
                     NMLLEASMALEQVTFETADHKEATMSFKEKRKPRFGQR"
     gene            39479..41680
                     /locus_tag="VKQ27_02210"
     CDS             39479..41680
                     /locus_tag="VKQ27_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLY87776.1"
                     /translation="MLRESLRGFLGEHWSADSVRRGPSSHEVAAIWTRLIGQGLATLG
                     SSRDEGGLREVAVVLAELGRAACPAPMWSAALANFALSTSTCESAVDCLSKLHAGSLR
                     LAFSFGTLDPDAGAGAIRVRGDHADGLLRFVEAAGSCTHLLVAIDRAVVGLVALDAAG
                     VEIVPTRAMGAWVLHEVRLDAVPVTIISLDDAVLDDLLIKGKLALAARAHGAARRAFE
                     LVTDYARERHQFGQPIGRFQAIQHKLANGLIVLEGVRLIVDHAAKLHDLGSRDWRYFA
                     NCAVAFASDALRRVSLETHHAFGAIGYADEHEAPLHFKRVHLDTIALGGGPHAKRSLA
                     SSLLDAGSSGLPEYDLGAAGNALREQVRDWLEQHWSGDRKAEFDREPFAKREFDADFA
                     RDIGKTGWIGLGWSREFGGQARSPLEQIALMETMERGEAPRIGAAIQANALMMFGTPE
                     QQQRYLPEILRGEAMHGMGYSEPQAGSDLAALRTSAVRDGNHWVINGQKIWTTTWWGK
                     YMFLAARTDKDAKPPHAGISMFIVPMNAEGITVRPATTMYDGSFANIFYDNVRIPLEN
                     LVGEVNGGWKVLTGALAFERGLVGGGIVLKVAHAFEQLRRHVMEKDAAGTALSDDPIV
                     RDRMAMLAAEIEIGRLLMMHCAELAAGGVTPPEYGAISKVFSGELMERFGEAALDILG
                     MRAALSEQMPGAIDNGRFEQNLRHSLMWVISIGTNEIQRSLIAQRALGLPR"
     gene            41691..42899
                     /locus_tag="VKQ27_02215"
     CDS             41691..42899
                     /locus_tag="VKQ27_02215"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY87777.1"
                     /translation="MQEQETANLPLEGVRVLDFSIMLAGPYCARLLADAGAEVIKIEP
                     PDGDDMRLRSPLRNGQSAYFGQLNAGKRSLALDLKSAEAIELVRQLVKETDILVENFR
                     PGVMDRLGLGYDALRAINPRLIYCSISGYGQTGLAAERAAYAMIVHAESGFDRSLMRY
                     AGDRDRPAAGAVFVADVLGGIFGYSAIQTAMVQRARTGVGQRIDVALMDCMLNLLVYE
                     LQEAQFPPQAPRAAYGPVRASDGDILIAPITPRNFAALCEVTGQGELANDPRFRTVPG
                     RAANWTAMMQVVEKWTEQHTVRECMAALDKAGVPSAEYRDPGAALTDPHLLQRGAFAT
                     IADSAGEFLGVNAPWKMSGGPTAMRREIPAVGAHRDDVLSRVLGLSGEAIASLAEAGV
                     FGSPNARSKG"
     gene            42932..44047
                     /locus_tag="VKQ27_02220"
     CDS             42932..44047
                     /locus_tag="VKQ27_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HLY87778.1"
                     /translation="MKIIAAIANGPRDPFEIGPCELSAPRAGEVLVRVHACGICHTDL
                     AVKLGHIPVPLPKVLGHEGAGVIEEVGPGVTSFRPGDHVLMSFGSCGECPNCRDGALG
                     YRDHFRAINLFGNRPGGSALRRGGSELGGHFFAQSAFATHAIATTRNIVRVDADLPLE
                     LLAPLGCGIQTGMGSVMNVLRPQAGTSIAVFGVGAVGLSAIIAARIVGCSTIIAVDVV
                     PERLRMARELGATHAVSGRADDPVAEIVELARGGENFSLDTTGVPAAVANSINCLRAR
                     GTSAQVAAPPRGTLYAAEASVVVGRGIAIRGVVEGDANPELFIPRMIGFFRDGRLPLD
                     KFVRTYPLARINESVDDLESGRVVKPVLTMREIGSTT"
     gene            44074..45309
                     /locus_tag="VKQ27_02225"
     CDS             44074..45309
                     /locus_tag="VKQ27_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY87779.1"
                     /translation="MLDIAFDNAIADHRTYGSVDLQHRLFGRLRREDPVHWTQPDGYR
                     PFWTITKYADIIEIERQPDRFINAPRTKLLSIEFESKVKEAMSGKPMLVRALPQMDNP
                     DHAKYQKLTHAWFQPRHIRTLSERMAALAKDSVDRLVAMGSACDFYNDIAIYYPLRVL
                     MTILGLPQSDEERLLKITQAYFGGGDPEMQKGSDLIDATIAYVDYFKDVAQERRRNPK
                     DDVATVIATSEIDGRPIEDFEAYSYCIALASAGHDTTSATIAGGLLALIENPDCMARL
                     QRQPDLLPEAAEEMVRWVSPVKHFFRTAAADYELRGKTIRAGDSLLISYPSGNRDEEA
                     FDRPFEFIPDRSPNRHLGYGFGIHACIGRHLAKAEVVAFFRELLSRVERIELAGEPAW
                     TETSFLGGVKRLPVRYSAR"
     gene            complement(45356..45850)
                     /locus_tag="VKQ27_02230"
     CDS             complement(45356..45850)
                     /locus_tag="VKQ27_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY87780.1"
                     /translation="MSAPKGTSASAADIARNWRLEGGIGFLLRLLEARYDVLYQDLTG
                     QSDITPRQFGVLIALYQDGPLTPSALAERISSDRNTLSEMLKRMTARKLISKRNNSED
                     RRSIQVQITAKGEEALLGVVRAAAELQTSLLAPLSKEDRAHFLKCMLAIAKAAAAPPP
                     SSKT"
     gene            46058..46378
                     /locus_tag="VKQ27_02235"
     CDS             46058..46378
                     /locus_tag="VKQ27_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87781.1"
                     /translation="MSLRELLSIPYLLEAEAVETEPGQWLVRLSYPELPGCISEGVVI
                     EDALKELERRRIETIVDLVETGREPPVPRKPLAAADPLWTANDLGLSGRVTALLGRTP
                     GQEA"
     gene            46406..47725
                     /locus_tag="VKQ27_02240"
     CDS             46406..47725
                     /locus_tag="VKQ27_02240"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY87782.1"
                     /translation="MLSREDNERLVRVGKGTPLGALFRLYWIPFLPSADLVNDGQPKR
                     IRLLGEDLVAFRDSEGRVGLVDQACPHRGAPMVFARNEDCGLRCVYHGWKFDVTGATT
                     DMPAEPVRSRLKERVRIKAYPCRERNGMIWTYMGPDQADLPPLPNVEWNLVPQEQVHI
                     SLRVQECNWLQAVEGEIDSAHAPLLHGRLDSQGTTSAWIQKRDLRPTFECIKQDFGMS
                     IAARRNYDANTLYWRVNQFLLPFYTLVPPLMPFPDLSGHAWVPIDDENTLCIMFSYHP
                     SQPLLPKTRQVFSEGHNGRESGHASRHALVQHPVTVPYAGYWTKYHPQNGFQFDYEAQ
                     KTTWFSGLPGLWVQDGACQSGVSPIFDRTRENLCSSDTGIAMTRRFILEALGAYRDHG
                     TKPKGVTDPDVFMVRAVSMRLPPEESWADVGAPFMRARLGADFGYEM"
     gene            47760..48722
                     /locus_tag="VKQ27_02245"
     CDS             47760..48722
                     /locus_tag="VKQ27_02245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HLY87783.1"
                     /translation="MSEGLIDFRVKRISFEVDGINSYELISPTGRDLDPFTAGGHIDL
                     HLPNGMIRSYSLVNDQRERHRYVIAINREPAGRGGSRFLHDNLRVGDIVSVSRPRNNF
                     ALCEQAEHSILIAGGIGITPLLSMARRLDALGRSCELFYAARARPAAAFLDEIEVLGS
                     SGCLKVHLDFDDERSGRLFDLSAIVERAPARAHLYCCGPQPMLEAFEAAAKDRPAGQV
                     HVEYFQAREAPAIEGGFDVRLARSNRTIAVKAGKTILDALLDAGIAANHACSEGVCGT
                     CETRVLEGIPDHRDQFLSKDEQASNKTMMICCSGARSGTLVLDL"
     gene            48755..49978
                     /locus_tag="VKQ27_02250"
     CDS             48755..49978
                     /locus_tag="VKQ27_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87784.1"
                     /translation="MKVALRILGMSFLIASACPLALADAARTMKIGILNDASGPYSDN
                     AGEGSVTAARMAAEDFMQQHPDFKVEIISADHQNKADVGAAIARRWIEQEKVDAVADV
                     PNSAVGLAVNEVVRGSKASLIASSAASSDLTGKYCSPNTIQWTFDTWAASHTIGNYLV
                     RHGGKKWYFIATDNALGKSMVRDGTSVISAGGGTVLGSVNVPINNADYASFILQADAS
                     GADVIAFATAGGDTVSLIKQSAEFGLKQNGRTFAALLTTMNDVEASGLAVGQGLIITQ
                     PFYWDLNDASRTWSAKFSARQHDQSPTAFHAGVYSSVLAYLNAALAAGTNDAQAVIRK
                     MKERPIDDALFGPVVVRADGRAIHNMYILKVKDSKASKGKWDLLENVGVLSGPEAFRP
                     IDQGGCKMVEQSKGN"
     gene            50032..51321
                     /locus_tag="VKQ27_02255"
     CDS             50032..51321
                     /locus_tag="VKQ27_02255"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY87785.1"
                     /translation="MLDNIEADFPEHTPRPSNAPKALEGIRVVDFSHFIAGPFATMIL
                     ADMGAEVVKIEAPGRGDDLRRYPPVHPELKHGAPFVWTNRNKQSVALDLKSPEGVAVV
                     RDLIATADVVVENFSTGVMERFGLDYEVCRKIKSDIIYCSVSAYGRDGAFADRLGFDP
                     IAQVESGFVSMNGYADREGVRALSPVMDISTAMMACNAILGALVARERSGLGQAIEVS
                     LFDNAVLMTGYATMQHLFSGANPQRHGNTSPDTCPSGVFQAQDCTFYINCGNDKIFQR
                     LMSQVIDRADLASAEIYATGPDRIRRRDELFAVLGEAFAHQPWLHWQARMRAAGVPCG
                     QVRSVGEAIRSAEARERGLVTRIPHELLGWIPNVNLPIRYSRTPIVDPVAAPAVGQHT
                     ERVLREQLGYDDARLARLAEAGAFGDAARSARGDVKI"
     gene            51318..52514
                     /locus_tag="VKQ27_02260"
     CDS             51318..52514
                     /locus_tag="VKQ27_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87786.1"
                     /translation="MNSRTLADRTLRGRVAVIGIGETDYYRHGGSPDPEFKLALKAIL
                     AACGDAGIDPRDIDSFSSYSDDRNEASRLAAALGTRRLRAATMQWGGGGGGSCAAVAN
                     AAASIVAGLADCVVVFRSLAQGQYGRFGQTTGIDTVSGEKAYLMPYGVLAPPQRFAVR
                     VRRYMHEHGVKQEALRAIALASYHHAQSNPRAVMYGKPLDAAKYDASRWIAEPFHLYD
                     CCMENDGAAALVLVPVERAKEFRSKAVYVLGAAVGSGYRTGAIPHNSPLYASASFDTV
                     APDLYGMAGLGPSDVGVVQCYENFTGGVAMALSEHGFFKPEEANQFLAFENLVAPFGR
                     LPLNTSGGNLAECYIHGFELVLEAVRQVRGTSTNQVKRNDVAMVIGAPMVTPVSNLLL
                     GSEATL"
     gene            52511..52963
                     /locus_tag="VKQ27_02265"
     CDS             52511..52963
                     /locus_tag="VKQ27_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLY87787.1"
                     /translation="MSEKTGLTYLPTGLPIPIAEADGLSAPYWEGLRQNRLLVQRCRH
                     CDTWQFGPEWICHRCHAFDPQWTEVEPRGLIFSWERVWHPSHAALRQHGPYLAVLVEI
                     PHAGGVRMVGNLLGDPVQIVSIGASVRGIFEHHSESAPPYSLLQWQTS"
     gene            complement(53303..53440)
                     /locus_tag="VKQ27_02270"
                     /pseudo
     CDS             complement(53303..53440)
                     /locus_tag="VKQ27_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(53545..53772)
                     /locus_tag="VKQ27_02275"
     CDS             complement(53545..53772)
                     /locus_tag="VKQ27_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY87788.1"
                     /translation="MSDPMFLTLNEVVERYRDEISEGTLRNWRSMRIGPSFIKIGKAV
                     LYPLEELDRWDRKNLVVCRPSRSLPLEEAAE"
     gene            complement(54123..55295)
                     /locus_tag="VKQ27_02280"
     CDS             complement(54123..55295)
                     /locus_tag="VKQ27_02280"
                     /inference="COORDINATES: protein motif:HMM:NF005077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HLY87789.1"
                     /translation="MTAAYIVSAARTAGGRRNGALAGWHPVELAAEVLNATLDRTGID
                     PSAIDDVIMGCVSQVGEQSFHVGRHAVLASRLPETVPAVTIDRQCGSSQQAIQFAAQA
                     VMSGTQDVVIAAGIESMTRVPMFSATKLAADAGMGTPFADHIRQRFGVAEFSQFTGAQ
                     MMAAKYGFDRETLDRFALESHRRAVAAIDAGAFDDEIVPIAVKDAAGNEHVHVHDEGV
                     RRDASLEAIGSVRTLQDRGVITAANSSQICDGTAAVLVVNEAALKAHGLTPLARIVNL
                     TVTAGDPIIMLEEPIRATRRALNRAGLSIDDIDLYEVNEAFASVALAWTREIGADPAR
                     LNVNGGAIALGHPLGASGAKLMATLVHALHKRSKRYGLQTMCEGGGIANVTIVEAL"
     gene            complement(55342..56118)
                     /locus_tag="VKQ27_02285"
     CDS             complement(55342..56118)
                     /locus_tag="VKQ27_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87790.1"
                     /translation="MTQYQTIVVERHGAVTLIRLNRPEALNALNSQVLTDLIDAFATY
                     EADDSRRCAVVTGNDKAFAAGADIKEMSSRGFSEMFGENYFGGFDRVTATRKPWIAAV
                     AGFALGGGCELAMMADFIIAAETAKFGQPEVKLGVAPGMGGSQRLAKAVGKAKAMDMC
                     LSGRMMDVHEAERAGLVSRVTPAATLVEDAIKAAAEIAAMPPLATIANKEMVNTAFEV
                     GLSAGLLFERRLFNGLCATADKREGMAAFVEKRKGAWAGK"
     gene            complement(56153..57076)
                     /locus_tag="VKQ27_02290"
     CDS             complement(56153..57076)
                     /locus_tag="VKQ27_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLY87791.1"
                     /translation="MSKVIISCAITGSIHTPSMSPHLPVTPAEIAENALEAAQAGAAI
                     VHLHARNPKDGRPDQSPEAFEPFLKVIKQSSNVVVNLTTGGSPYMPVEERVRPAAVWK
                     PEVASLNMGSMNFGLFPMLKRYKEFKHDWEPAMLEGSHDLVFRNSFKDIRYALETLNE
                     TGARYEFECYDTSHLHNLHYFWTEGLVQAPLFIQTCFGLLGGIGAHPEEVLHMKRTAD
                     RLFGDNFKWSVLGAGRSQMPVAAQAAAMGGHVRVGLEDNLWLDKGVLAPSNAAQVTRV
                     RHIIEGLGLEIATPDEARDILKLKGGDKVGF"
     gene            complement(57080..57847)
                     /locus_tag="VKQ27_02295"
     CDS             complement(57080..57847)
                     /locus_tag="VKQ27_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY87792.1"
                     /translation="MDLKNKVAIVTGGGSGLGEATAIKLAEGGAKVAVLDLNGDAANA
                     VAERIGGRAFAIDIADAEAGETTVATIASELGTPRILVNCAGVGTPMKILGKDGPVSL
                     AAFEKVVRINLIGTFNMMRLVADVISRDQESGDGKQTQGVIINTASVAAFDGQIGQPA
                     YSASKAGVVGLALPAARELARFGIRVLTIAPGIFLTPMLKILPDEALESLGRSVPFPP
                     RLGEPEEFAKLALHMIDNDMLNGEVVRIDGSIRMAAK"
     gene            complement(57876..58658)
                     /locus_tag="VKQ27_02300"
     CDS             complement(57876..58658)
                     /locus_tag="VKQ27_02300"
                     /inference="COORDINATES: protein motif:HMM:NF005752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87793.1"
                     /translation="MSLDPQCLSGRNAVVVGASSGINLGVAHRLGSLGAQIAIVSRSP
                     ARIAAAADTLRADGLDVTHHAGDVREATTMQAIMTEVADTKGPIDIVLSGAAGNFFVP
                     AAKMSPNAFKTIIDIDLLGTFNVLSAAYPHLKRPGAVLVNITAPQAARAMPMQAHACS
                     AKAGIEMLTRCLALEWGVEGIRVNTVSPGPIADTEGLARLATDAELAERIRGALALPR
                     FGQRIDIGNAVAWLCSEAASFVTGTTIDCDGGMRLGNLYAPA"
     gene            complement(58677..59429)
                     /locus_tag="VKQ27_02305"
     CDS             complement(58677..59429)
                     /locus_tag="VKQ27_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLY87794.1"
                     /translation="MDSIARIILARPDAGNAINAALIADLGAAVDAVRACEPRSVLIT
                     AEGANFSLGGDLKHLLAARSDVAAELDRMAHGFHAAQLRLIGIGVPIVAAARGHVIGG
                     GFGLALSSDYLLCSENTRFSTGYSRLGLSTDAGVSYFLTRALGARRARTLLMDARFMS
                     ADEAVVLGVADRKVSDDKLDAAALDLARELAEGPTAAYAAIRRLTDAAATNSLATHLD
                     EETREIVALAGREDVRAALAAPLTRTKAVFTR"
     gene            complement(59547..61097)
                     /locus_tag="VKQ27_02310"
     CDS             complement(59547..61097)
                     /locus_tag="VKQ27_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87795.1"
                     /translation="MYPGVHAKIRTDQPAFIMAQTGVAVTYAELEQRTNRLAHFLRSR
                     GFKRLDHYAIFMENNDRYIESCGAGERAGLYFTCVNSFLTADELAYIVNNSESGILIV
                     SEETRATALTAIGQCPKVEAVLVVNGPGDGDRILNLDEATASFPATPIADETIGTAML
                     YSSGTTGRPKGILRPLPDQPPSQQLPLFDFLQKLWRYSEGLVYLSPAPLYHAAPQASV
                     NLVIRNGGTAIIMERFDPEHYLQLVERYRPTHSQLVPTMFSRMLKLPEATRSSYDLSS
                     LEVIIHAAAPCPVQVKEQMIAWWGPIIHEYYGATEGLGFAACDSEQWLAHRGSVGKVL
                     LGELHVLDENMQPCPKETPGTLWFKTATPFEYFNDPAKTKEARSQDGSMSTVGDVGYV
                     DDDGFLYLTDRATFMIVCGGVNIYPQECENLLITHPKVADAAVFGVPNEDLGEEVKAV
                     VQVVPGIEANSDLVNELVNFCRAHLSHQKCPRSIDFEAELPRLPTGKLYKRLLRDRYW
                     GDRRTRIV"
     gene            complement(61341..62939)
                     /locus_tag="VKQ27_02315"
     CDS             complement(61341..62939)
                     /locus_tag="VKQ27_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87796.1"
                     /translation="MRPITSKSSSGAADWQLDAAIAADITLDALFRRAFRVFADRTAD
                     TWEGGQRRYREVGERAWRLANALRAKGYDRGDTIAILSETRPEYVECYAAMAALGITV
                     ATLNIRLRPDEIAACLRKARPSAVLTSGTLAALLEGLRSEFPDVGDWIAFEPAQGFDD
                     YDAILAESSAGEPPRIAEGADIHNILFTSGTTGQPKGALVSQRAAATRGLRLAQWFGL
                     TEKDGFVGWLPLFHCGGDESLYATFVTGGIYATFKRVDIPAMYERIERERLSWTLLLP
                     GVITDFMNNPQRQAHDLSSLRFAIGYANMMPNVVAKLTETFGIDFYDAFGQSESSYLL
                     AHGCSGPGEVPSLRKRPSPLMEVRVVDEDMNEMPVGQPGECVVRGPSIMSGYLDDDAA
                     NAEVFKGGWLHTGDLLRREEDGTLTFVDRKKYLIKTGGENVYPAEVEAVLSRHPAVQE
                     ACVFGVPDPHWGETIKAVVVLRQGQSADGAEILHWCRERLAGYKRPRYLQFMRSEELP
                     RSTTGKLQRHELACLPLTAEQRVG"
     gene            complement(62920..64509)
                     /locus_tag="VKQ27_02320"
     CDS             complement(62920..64509)
                     /locus_tag="VKQ27_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87797.1"
                     /translation="MDVSSHLIHRVNVGDLVTRSAERFPEREMICDGDRRWSYRDFNA
                     WTNRIAHGLSALGLQKGDALALMSGNRAEFLAVYFACARLGLVCVPINLLWRQHELSH
                     VLTDSRARAAVVETGLLQQFCAVVEGPGPLLGIVVMDWQASGASTPDGIGDMPLFDFD
                     ALAADQTSDIPDVEIEDRAPLSYLYTSGTTSAPKGVIGTHLAIYLESLGTAIDTRMTE
                     NDRLSVVLPLFHTAQLNALCTPAIAVGASMVIQRGFDAVGLHEAIERERISVLFALPM
                     MIQQLVERQLAHPRNVSSLRLTVYAMAPMPQAELRRAIEIFGCDFSLMFGQTEMSPVS
                     TFFKPQHQLSHPGAVGTPAVNVQVAIMASNGELLPTGSTGEIVYRSPQTMAGYLHNPE
                     ATAEAFRHGWFHSGDAGHFDVDGILWFEDRFKDVIKSGGENVSSIEVELAIRAADPDV
                     LEVAAIGLPHRHWGEAITAVVVTQNGGSIDANMLLSAVRERLSPFKCPKAVIHVSELP
                     KTATGKIEKAKLRRTYAAYYQ"
     gene            complement(64553..65434)
                     /locus_tag="VKQ27_02325"
     CDS             complement(64553..65434)
                     /locus_tag="VKQ27_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87798.1"
                     /translation="MADNQTPERPDGLGLTDDELAVHRTAFASDALRDMIVVLSGGAG
                     GIGRATAWLFARLGAHVVLVGRNQERLDTLVSNLTGRKLKASAYGADIRDPDAVKALF
                     DAAWKTHGRVDVLINSAGGQFPQPAIDFSVKGWNAVINTNLNGTWYMMQAAAQNWRDH
                     KYPGSIVNIVVVTTHGLYGVAHTIAARSGVIGLSRSVAVEWAPLKIRVNCVAPGAIET
                     EGWKVYTPEARTAYPRSNPMMRAGSPWDVAEACVYLAGPSGGFVTGETLTVDGGGQLW
                     GETWTTGKPPYFSGEDP"
     gene            complement(65427..66650)
                     /locus_tag="VKQ27_02330"
     CDS             complement(65427..66650)
                     /locus_tag="VKQ27_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87799.1"
                     /translation="MTTSHAILSGDNRASMFALDEEQQTILDQADRFARKELYPLSER
                     MDREEWWPDEAFPKIGDNGFFGATIPEEYGGAGMDLLAAGLVLQGFARWNHAMALSWV
                     AHDNLCANNIFRNGNEQQRRKYLPDLCTGRKIGALGLTEPGAGSDALGSMRTTARREG
                     DHYVLNGGKMYITNGPVADVILVYAKTDRAKGAQGISAFIVEKDFPGFKVAQKLTKMG
                     YRGSQTAELVFEDCRVPADNLVGGEDRGVSIVMSGLDLERAMISPLCLGISERALQLS
                     IDYAQTRQQFGKPIGSFQMVQSMLAEMYVQVETMRTFTYRTLAEAAPLEVGGGGRGDI
                     HMLTAASVMYAAEACHAVLDKAVQIHGGSGYIWESEINRLFRSIKLLEIGAGTTEVRK
                     MIIAGELLKDMATHG"
     gene            complement(66865..67320)
                     /locus_tag="VKQ27_02335"
     CDS             complement(66865..67320)
                     /locus_tag="VKQ27_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HLY87800.1"
                     /translation="MGRYYEEFEIGACFATPRRTILDADIVQFAGLTADFNPLHTDDV
                     FAAQSDFGGRIAHGPMLVGMAFGLASRAGLLDGTALALLNIGWTFTGPVRPGDTILTR
                     IHVVAKRPARKPDRGVVELLLDIVNQRDETVQTGTAKILMKRQSAVAAG"
     gene            complement(67324..68961)
                     /locus_tag="VKQ27_02340"
     CDS             complement(67324..68961)
                     /locus_tag="VKQ27_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY87801.1"
                     /translation="MIPIDETNTIGDAFARATQAYAPRAFLAAPAGAHRSYHRQGYEI
                     DFATAAREVRQLCEIYRSHGFGHGHRVAMLLDNRPEHFLHKLALNTLGISCVPINPDY
                     RANEIAYLLENSEADLAIVSGERRKQLTAGMDAGSHKAPVAQFDDLRSLGAPARPRRN
                     DPVTAASEASVLYTSGTTGRPKGCILSHGYELESGAWYATRGQLASFRAQGERIYNPL
                     PVYHVNSSIVSFHCAMLNGSCQIQPDRFHPNRWWPEVNQTGATIVHYLGVIVPLLLGM
                     PKCDEERSHSIRFGLGAGAEPQLHGVFEQRFGFPLIEVWGMTEMVRVLLDHEPPRKVG
                     TRAFGRPTPGIDVRVVDEQDQDVMVGAPGEMVIRHSEATPRRGFFSGYLKDDQTTAQA
                     WRGGWFHTGDTVRQDDDGMLHFVDRKKNIIRRSGENIAAAEIEAVLQSHDMVKQVAVL
                     AVPDELREEEVFACVVLADAHDTDVAADVLFQHCYAELAYFKTPGWIHFVDVLPTTGT
                     QKIQKHHIFPDGVDPRTIPGVFDMRQRKKRSSKTAGD"
     gene            complement(68982..69866)
                     /locus_tag="VKQ27_02345"
     CDS             complement(68982..69866)
                     /locus_tag="VKQ27_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLY87802.1"
                     /translation="MTLRSFLFTPGDSLKKMEKGAAGPGDALILDLEDSVAEPNRPGA
                     RVLVRDFLTARSPRSRQELWVRINPLDTPHALTDLAAIIASRPDGIMLPKVTSGRDVT
                     TLSNYLDALERAFDVPAGHVKILPVATETPQALFSLDKYGGASARLYALTWGAEDLAA
                     ALGASANRNQDGRLTFTYQLSRSLCLAGAAAADVVAIETVFPDFRNRAGLQAYAAASR
                     RDGFRGMMAIHPDQVEIINSAFTPSDQEIAWAQRVVAAFEGSSTGAVGLDGQMLDMPH
                     LKQARRLLEVAQLTMRSE"
     gene            complement(69863..70315)
                     /locus_tag="VKQ27_02350"
     CDS             complement(69863..70315)
                     /locus_tag="VKQ27_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLY87803.1"
                     /translation="MAGKFFDQFVDGEIIRHQVTRTVTETDNLLFTALTHNTQPLHLD
                     ADIAAKSEFGHILVNSIFTFGLMIGVSVSDTTLGSLVANLGMDKVVFPKPVRIGDTLR
                     SETKVVEKRESRSRPGQGIVVFEHRCLNQRNEVVAQCLRSVLVKRSPQ"
     gene            70545..71306
                     /locus_tag="VKQ27_02355"
     CDS             70545..71306
                     /locus_tag="VKQ27_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87804.1"
                     /translation="MTSRDGAVAVIRLNRPDRLNAVDEAMITELRAVLEAANLDPDVR
                     ALTMIGSERSFTSGADPTLFHRDLDRAPETAARLVDAFHGMLRSVRAMPKPVIARLSG
                     SVVVGGLGLALACDLCVMADNARLISAYSKIGTNPDAGTTWSLTHHLGRRRSLEVMWL
                     NDPIGAQEALSLGLVNRVVPQSRLESETLAVAKRIADGPPGTTGRIKSLVDQACVSDL
                     AAQLDREKAEFVAAAGSREFREGITASFEKRPAQF"
     gene            71415..72086
                     /locus_tag="VKQ27_02360"
     CDS             71415..72086
                     /locus_tag="VKQ27_02360"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY87805.1"
                     /translation="MARALLVDNDAVQVSIARLPRSSDMPAISPERMQDRYDAILDAA
                     KRAFAERGFEGASIADIARIAAISDGLVYRYFRNKRELLYGVLQKFYERILVDLELQV
                     FKHDGFTARLEALIKRHIEVFVSDTDLCRLFIAEVRTASDYEGSAIQELNRRYTSVLI
                     RIVKDAVKTGEVRVDVNPRLLRDVLFGAIEHLAWRHVNGRGQLRAASTARELTSMLTS
                     GICID"
     gene            complement(72111..72353)
                     /locus_tag="VKQ27_02365"
     CDS             complement(72111..72353)
                     /locus_tag="VKQ27_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87806.1"
                     /translation="MQRAGAGGSRRGNDLLAVQIRRDRCAARDRDRLVCQRHRGRPRI
                     DLVIDHDAAQPELAQRPDDPDRDLAAVGNQDAVEGE"
     gene            72453..73586
                     /locus_tag="VKQ27_02370"
     CDS             72453..73586
                     /locus_tag="VKQ27_02370"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLY87807.1"
                     /translation="MGDKIRARNFVARNEFPVAPSAIEEEDPATFVERARAIGAPLLV
                     KPSAGGGGKGMRIVRDLSGLDDAVAQARSEGQRYFGDGRLYIERFVEQPRHIEVQVLG
                     DAFGNVVHLFERECSVQRRFQKIIEETPSPALSAQTRQKICETAAGIARAAGYRNAGT
                     VEFIYGRGEFYFLEMNTRLQVEHPVTEMITGLDLVAEQIAVAAGRRLPFAQGDVRAHG
                     HAIEARLYAEAPERNFSPTTGKVLALRYPQGEGVRVDSGIAQGQTITTAFDPMLAKIV
                     VHATTRDEAIARARRAIEEFVLLGCETNAAFLARILGDQGFKAGEVHTGYLDENPGIA
                     RGGSRDTDAETRLLALAALLTRPVRDAADAVPPLHASLGSWRN"
     gene            73567..74091
                     /locus_tag="VKQ27_02375"
     CDS             73567..74091
                     /locus_tag="VKQ27_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLY87808.1"
                     /translation="MEAGETEVHHRFDLDGEINELWLSRGPAGYHLQIADGWSGSVAL
                     IEERAGHGRLVVDGKSEQVAYVVDGDVVHIHCRGRAYAVRYVDPLLALASASDEAGHN
                     VARAPMPGVVVSVKVTLGDTVAAGAVLMVIESMKLETPIRAPQDGVIERIHVRDGESF
                     DRDAVLATLSQGNG"
     gene            74093..75700
                     /locus_tag="VKQ27_02380"
     CDS             74093..75700
                     /locus_tag="VKQ27_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLY87809.1"
                     /translation="MQRLRSLTDTRADDFRSNDGHNRRLAAELKERQRIARFVRPERD
                     IERLGRQNKMFVRDRIEALLDPDTPFLELSTLAGNMAYDGEVPGAAQVIGIGIVSGKE
                     VIIHADDASVKGGAWYPLSVKKIVRALDIAIENRLPVVHLCDSAGGFLPLQAQFFADR
                     YYAGRIFRNQSVLSKMGVPQVAVVMGHCTAGGAYIPALSDYNVIVRGTGAIFLGGPPL
                     VKAATGEEVSAEELGGADMHTSVSGTGDYPAASELHAIAIARDIVGRFAHPEKSKIDW
                     VDPEPPAYDPDELYGIIPKDPRVQFDMREIIARVVDGSRFHEYQPRYGQSLVCGFARV
                     FGYQVGILANNGVLFSDSALKGAHFIQLCDKNRTPLLFLQNITGFMVGREYERRGITK
                     DGAKLIMAVAGASVPKFTVVCNGSYGAGTYGMAGRAFDPRFMFTWPQSQVSVMGAEQA
                     AGVLTDIKLRQLARNGQELSEQEHAAIRDPILEDYRLQSSAYYATSEIWDDGILDPVD
                     TRNALGIALSASLNAPIAPPHYGVFRM"
     gene            75919..76095
                     /locus_tag="VKQ27_02385"
     CDS             75919..76095
                     /locus_tag="VKQ27_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87810.1"
                     /translation="MTITRFDFEGLEGGRVVMTGTAAVTKANPDIQAAYLGGRHGTDY
                     LAVKHYRQRRRWLS"
     gene            76172..76555
                     /locus_tag="VKQ27_02390"
     CDS             76172..76555
                     /locus_tag="VKQ27_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLY87811.1"
                     /translation="MGMPNKMIVTCAVTGSIHTPSMSPYLPVTADEIAEAAIGAAEAG
                     ASILHLHARHPQTGKPDQTPQAFEPFLKIHQAAVELYRQPDDGRKPVHVRGRTIEACG
                     DVPAGGCVAQHGIDEFRSVPNAPAL"
     gene            76446..>76786
                     /locus_tag="VKQ27_02395"
     CDS             76446..>76786
                     /locus_tag="VKQ27_02395"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLY87812.1"
                     /translation="MSVEERLRPAATFQPEVASLNMASMNFGLFPMLQRYSEFKHAWE
                     PEMLEASRDLVFRNSFKDIEYVLSTMSQSGTRFEFECYDTAHLYNLHHFIELGLVKPP
                     LFVHVRDSRWHR"
BASE COUNT        13833 a        23150 c        25071 g        14732 t
ORIGIN      
        1 cctcgaccag gccgtgcggg aaatcagcga gcgatccgga gggcaggtct cggctctact
       61 tgccaatgtg cgcgatcccg acagcatcga ggcgatgatg accacaatct gggccgatgg
      121 tccgctcacc ggtctcgtca acaatgcggc cgccaacttc ctggcgccga ccgaaaccct
      181 gtcgccgcgc ggctatgagg ccgtccgctc gacggtcatg gacggctcgt tttacacctc
      241 gctcgcctgc ggcaagcggt ggattgccgc aggcctgccc ggagtcatca tcggcaacct
      301 cgtgacctgg gtgtggaccg gctcggccta tgtcgtgccg gcggccatgg cgaaagcggc
      361 cgtgcacgcc atgaccatgt cgctcgcggt cgagtggggg cccaagaaca tccgcgtcaa
      421 tgcgatcgcg cccgggccgt tcccgaccga aggcgcctgg gacaagctaa acccgctggc
      481 ggagaccggt gtcggcgcaa cgcaggcgga cgacgtgccg ctgcgccgtt tcggcaagat
      541 ggacgagttg cgcaatcttc tgatcttcct gatgtcggac ggttgcagct acatcaccgg
      601 cgatacgatc gccattgatg gtggtcatca tctggccgcg cccagcacct tcgcggggct
      661 gtcgaaactg tcgccagccg actggcagcg cgcgcgtgag gccattcagg cctcggtgca
      721 gaaggaaaag caggcacgct cgatctaagc cgctggttcg agccgccgcc aggttgcgtg
      781 aaagcgaggg agagacgccg atgggactgg ccacaccgac aaagagcttc gattgcacca
      841 tcgaggatgg cctggctcat gtcgtcttga accagcccga tcgtggaaat ccaattgatg
      901 gggagttttg tcgcgaattc ggcctgtgta tcgcggagct gagcgagcgc tccgatgtga
      961 gggcgatgct gctctccggg cgcggccggt tgttcagcgt cggcggcgac ctgatgtcgc
     1021 tggtcaagca gggagacgca ctgccaagaa cgatcaaggc ctggacggga gaccttcatg
     1081 tggcgattgc aagaatggtc cgcatgcggg cgccggtcgt cgcgagcgtt cacggcaatg
     1141 tcgccggtgg aagcgtttcg ctgatggcgg ccgcagatct ggtggtgata gcggaatcgg
     1201 cgcggatttc agctgcgttt tccaaaatcg gattcagtcc cgacagcggg tcgaccacga
     1261 caatcacgcg ccggatcggc gttgcgcgcg ctaggcgcct cttcctgctt ggcgagacgc
     1321 tcgatgccag aacggcgctg tcgctcggtc ttgtggattt cgttgttccg gatggcgatg
     1381 tgcagtccga ggctgcgcga atagcgaaag agcttgccgc gggtccgacg caagcgtttg
     1441 ccgcgatcaa gcgtctgttt gccgagacat ccgatcgatc gttcgagagt cagctcgagg
     1501 aggaggcgga gacgctcgcg acaatctcgc gaacggcgga tgcgcgtgaa ggcgtcaagg
     1561 ctttcgtcga gaagcgaaag ccggctttcg tcggggaatg acggcaacca actacacgcc
     1621 gcaagcgaca aactacgaaa tagttcgtga tcataaagta cacagataaa cgaaagccaa
     1681 gtcggaaaag acacgacgct gcaggaggga ataatgaccg tggcggccgc cagaaatgat
     1741 gcgtcttacg ccaattttcg cgaagccggc tattggctcg acaagactgt cgatcagctt
     1801 ctgaccgagg ccgttgcgag aacgcccgat aaggtcgcga tcgtcgccga ccgcgcagat
     1861 cgagaacagc ccccgcgttt cacttacaag gaactggaga agcttgcaga tcgcgccgcg
     1921 agctcgctcg tgcggctcgg cgtcggacga ggcgatgtcg ttacggtgca actgccgaac
     1981 tggtgggagt tcgtcgtcac cgcgttcgcc tgtagcaaga tcggcgcggt gatgaacccg
     2041 gtgatgccga ttcttcggga gcgcgagcta ctctacattc tgaacttctg ccaggcaaag
     2101 gtcttcatcg tgccgaaggt ctatcgcggc ttcgactacg ctgcgatggc gcagggcatg
     2161 cgtgcggacc tgccgcatct caagcacgtg attttcgccg acggagatgg cgagaccagc
     2221 tttgatcgca tgttgctggc ttccgaggcc gacaggcttc ctcctggtct ccgcccggat
     2281 gatatggccg tgctgatgtt cacgtccggc accacgggcg agccgaaagg cgtcatgcac
     2341 acgtccaact cgttgatcgc gtgctgcaag gccctgtcgg gacggttcgg gctcgattca
     2401 agtgatgtcc ttctggtggc ttcgccggtc ggacacatga caggctatgc cgcgatcgtg
     2461 ctgctctcgg tctatctggg cggcacgatg atcctccagg atatctggga ggccaagcgc
     2521 ggggttgggc tgatggcccg cgaaggcgtg acctatacgg ccgcgtcgac gccgttcctc
     2581 agcgacatct gcgacgcggt aaaggccggc gcacctcagc cgaagagcct tcgctccttc
     2641 ctgtgcgggg gcgcgccgat cccatcggtc ctgatcgacc gggccgccga cgagcttggc
     2701 cttaaagtct gctcgctctg gggcatgacc gaggtcttgt cgggcaccct gaccgagccg
     2761 gcgcgcgccg cggagaaatc ggccggcacg gacggccgct cgctggaggg catggaggtc
     2821 aggatcgtcg ataccgaggg tagggcgctg ccggcgggag agtcgggccg gttgctggtg
     2881 cggggcgccc agatgttcaa aggctactac aagcggccgg agctgccgac cttcgacagc
     2941 gaaggctggt tcgattccgg tgatctcgcc tatatggaca aggatggcta tatccgtatc
     3001 tcgggacggg tgaaggatat cctgatcagg ggcggagaga acgtcccggt cgtcgagatc
     3061 gagaacctgc tctacaagca tccagcggtg tccgccgttg cggtcgtggg attcccggat
     3121 tcacggctcg gcgagcgtgg ctgtgccttc atcgtccccc gcgacgggag cagggtcgat
     3181 ctcgcggcgg tgcaggccta tctgggtgaa gccaaaatgg caaagcagtt ctggcccgag
     3241 cgggtcgagg tcgtggccga gcttccgcgt accgccagcg gcaagatcca gaagttcaag
     3301 ctgcgcgagc tcgccacggt ccttgccgat gcaaagtaag ctactgatcg gaataggtcg
     3361 gattccgtcg ctcgcgaaac gcggcgacgg cttccgcgtg gtcattcgtt tccaggagga
     3421 tgacctgctg ccggtcttcc atcatcaggg ccgcgtcgag gctcggcgcg tcgattagtg
     3481 tgttcagcgc ctgctttgtc atgcgcagcc ccattggaga cacgcgcagc atctcctcgg
     3541 cgaattcgag tcctttcgcg agcagctcgt ccgcgggcgc gatatcgctg actagtccga
     3601 tcgctttcgc gcgctccgcc ttgaggaaac gtcccgtcag cagcagctct gaggtcacgg
     3661 agaggccgac gaggcgaggc aacaggtatc ccgagcccat gtcgcagccg ccgacgccga
     3721 tgcggatata ggccgcgttc atccgcgcat ccggtgctgc gaagcggacg tcgcaggcga
     3781 gcagaaagga gaagccggca ccgcacgccg caccatggac gagcccgatg atgggctgcg
     3841 ggcacgaccg catgaggcgg atcacgcccg agtaattctg ctgcatcttg agctggcgtt
     3901 gcgggcgacc cttgcccggc gcggcgaatg cgtccgatcc gagctcagcg ccggcggaga
     3961 actgaggtcc gttcccgcgc aggatcagga cgcgtgtccc tggccggtcg tgaaggccgg
     4021 agaagtacgc agtcaactca gagatcatgt ctggcgaaac ggcattgagc tcggccgggc
     4081 ggttcaatgt caggacgtcg atcgcgccgc gggtttcgac gatgatggtg ctcggctgcg
     4141 tcatgggctc gctcccatcc cttgttgtcc ttgagatcag atagtaataa agtgtacgat
     4201 catcaagcgc aatgccgtcc caatcccttg aaggcaaggg tgacggcccc gcgatacgag
     4261 ctcagggagg ccggagatgc catatcagtc cgtgttcaga tcggacctgt tcaagggaca
     4321 gacgatcatc gtgaccggcg gcggcagcgg aatcggacgc tgcaccgccc atgagctcgc
     4381 cgcccttggc gcgaacgtcg cgatcctcgg ccgtaccttg gacaagctcg tcgaggtgca
     4441 gcacgagatc gaggaggatg gcggacgggc gatgagcctc gcctgcgaca tccgcgatga
     4501 agccatggtg atcggcacga tcgatgccgt tctggcgcgc tatggccgca tcgacggcct
     4561 ggtcaacaat gccggcggac aattccgcgc gccgctgaag tcgatttcga ccaagggctt
     4621 cgaggccgtc gtgcgcaaca atctgaccgg cggcttcatc ttcatgcgcg aagtttataa
     4681 ccgctggatg gaggccaatg gtggctcgat cgtgaacatc attgccgaca tctggcacgg
     4741 ctggccggag ttcggtcact cggccgcggc gcgcggcggc atgctgacct tgaccgagac
     4801 ggccgcctgt gaatggtcgt cctcaggcgt gcgcgtcaat gcagtcgcgc ccggcggcat
     4861 cgtgtccagt ggctttgata cctacacgcc ggagatgcag aagaagcttc acgacttcac
     4921 cgaacgcgtg ccgctccagc gcttcggaac ggaagccgag atatcggccg caatcaccta
     4981 cctgctgtcg ccggcagccg cctatatcac cggctcctgc atccgcgtcg acggcgggac
     5041 gccaaatgca cgcacgactt ggaagctcga gccgcatagc cgcagccagc cgttcgaggg
     5101 cttccatcgc acgaaactgc ctgaagtgat gaagcgcaag gccagcgcgt agaggcctaa
     5161 tccactgcga tgatcagctt gccgtggttc tccccgcggt agagttgtgc gatcgcgccg
     5221 ggggcatgct ccagcccggg caggatttct tcatcatgga cgatcctgcc ggccagcgcg
     5281 agttcggcaa ggcgcatggc ggccgcttcg aactcggcga gatgatcgaa gatgatgaag
     5341 ccggaccacc gcaggcgccg cgtgaggatc tcccgttcgg ggcgcgggcc ggtcgggacg
     5401 ggggtccagg aggcgttcgc ggccgtgccg cactggatga tccgtccacg cagccgcatg
     5461 gtccggatgg cggcatcggc gatcgcgcct ccggtgttgt cgaagaagat atcattgcct
     5521 tcagggcatg cggcccgaat ggccgcgccg agatcggcgg cctcgcggta gttgatcatg
     5581 tcctggtagc cgtagcgggc cttggcttgc gcaatcttgt cggcggagct ggtaaggccg
     5641 acggcgcggc atccggcgat ttgcgcgagc tggccgacga agcttccgac gctgccggct
     5701 gccgtcgaca ccagaacatg ctcgccgggc ttgggatcgc agaggccgtg aaaggcaaga
     5761 taggcggtca ggccgttcat gccgagcacg ctcaaattgg cgctgagcgg cagggcggat
     5821 ggagtaacgc gccgcagaac ggtgtcgggc gtggtgacgc agtagcgctg ccaaccaaac
     5881 catccataga cgaactcgcc ggcgcgaaac tccggcgtat tgctttcgac gacctcgccg
     5941 acggcgaggg cgcgcatcgg cgtgttcaat ggcaccggcg cgctgtagtt cccctcgtca
     6001 ttggcccatc cgcgctgcgc cggatcgacc gagagatagc ggttttcaac cagcatttcg
     6061 cccttgccgg gcgcggccac cggcgcggtc acgagcgaga aatgttccgc ttgcggtatc
     6121 ccatttggtc gggacgtcag gcggacctgt cgattttcgc ggacggacat gagcgatcct
     6181 cataattagt acgcttgtgt acgatatgac tatacggtag aaatcggacg ggagacaagg
     6241 acaaggcggc cgccacggcc gccgcctgac cctgcaagat acagcaagag gcaagcacat
     6301 ggcggggctc tacttcgagc agttttcggt cggacagaca ttcgtgcacg aaatccggcg
     6361 cacggtgacg gacatggaca acatcctgtt ctcgtcgctg acctacaatc cggcggcggt
     6421 ccacatcgac cacgaatatg ccaagggcac tgagttcggg aagcccttga tgaattcgat
     6481 cttcacgctt ggcctgatca tcggtctgtc cgtccaggac acgactctgg gaaccaccgt
     6541 cggcaatctc ggcatggagg ataccaaatt cccgcgcccg gtgtttgccg gtgacaccct
     6601 gcgagccgag accaaggtga tcgccgtcag ggagagcaag tcgcgtccga cgcagggtat
     6661 cgtgacattc gaacatcgcg gcttcaatca gcgcgacgag gaagttgtct actgccgcag
     6721 gagcggtttg atgatgcgga ggccgacatg aagctccgtt cgctgctctt tgtgccggcc
     6781 gacagcgagc gaaagtacat caaggcctcg ggcgtcggcg cagacgcact gatccttgat
     6841 ctcgaggact ccgtggcgcc ggggcgcaag gcctttgccc gggatgccgt gaaagggctg
     6901 ttgggcggcg ggccccgcaa ctggtcgttc ctggtccgca tcaatccgtt cggtacgggg
     6961 ctcacgctcg aggatctcgc ggcggtggtc cggccgggcc tcgacggcat cctcatcccg
     7021 aaggtcaacg gcatcgagga tgtcaatctc gtgtcgcact atgtcgatgt cctggaagtg
     7081 gcaaccggca tctcgccggg gcacgtgaag ctgctggttg tcgcgaccga aacacctgcc
     7141 gccatgatcg gcttcaacgg ctacgcccgc aagaacgagc ggctggtcgc gatgacctgg
     7201 ggcgcggaag acttgagcgc ggccttgggc gcgctcacga acaaggaagc ggatggcgcc
     7261 tggaccttcc cgtaccaggt agcccgcgca cagtgcctgt ttgccgcggg tgcggcaggc
     7321 gtcgcggcgc tggatacgct ttatgcggac ttcaaggatc aggatggcct cgccgaaagc
     7381 tgtcgcctcg cgcgccgtga cggtttcgtc ggccgggtcg cgatccatcc ggatcaggtc
     7441 gcgaccatca acacttgctt cacgccgtcg gatgccgacc ttgcgcacgc gcgccgggtc
     7501 gtcgcggcgt ttgccgcggc gccggacgtc ggcacggtcg gcatcgacgg gaagatgtat
     7561 gacattccgc acctggtcgc agcgcggcga accttggcgt cggtcgggga ggagcattcg
     7621 aatggacaat aggtcgttct ccaatcaact cgatgagccc gaggaccacg ccgccatccg
     7681 cgagggcgtg cgcgcggtcg taacgcgttt cgatgacgag tactggctgg cgcgcgacga
     7741 tgacggcgag ttcccgcgcg aattccatcg tgcgatggcg gaagccggct ggctcggcat
     7801 cacaatgccc gaggagtatg gcggctccgg gctgggcgtc accgaagccg cgatcatgat
     7861 gcatgaggtc gcaagccacg gtggcggcat gacgtcggca tccgccgtgc atatcaatct
     7921 gttcgggcca cacccgatcg tggtgaaggg gaccgatgag cagaagcgac gctgggtgcc
     7981 gcgcctggtt tcgggcgagg accaatgctg cttcggcttc accgagccgg atgccgggct
     8041 gaacaccacg cgcatcaaga ccttcgccga gaaagtcccc ggaggctacc gggtgcacgg
     8101 acagaaggtc tggacgtcga cggcgcaggt cgccaacaag atcatgctgc tgacccggac
     8161 cacgaagttc gaggactgca agcggccgac cgacggcatc accatcttct ataccgatct
     8221 cgatcgctcc aagatcgagg tgcgccgtat cccgaagatg gggcgcaagg ccgtggattc
     8281 caacgctatc ttcatcgacg gcctgttcat tccggaggcc gataggatcg gtgaggaggg
     8341 gaaggggttc tcctacatcc tgcatagcct caacccggaa cggatcctga tcgcggttga
     8401 agcgattggg atcggacagg atgcgcttcg acgggcaacg cagtacgcca aggaacgtgt
     8461 cgtgttcgac cgtccgatcg gccagaacca agggatccaa catcctctgg ccgagaagtg
     8521 gatgtatctg gaagctgcat ggttgatggc catgcgtgcg gcgtggctat acgataccaa
     8581 caagccgtgc ggcgccgagg ccaacagcgc caagttcctc ggcgcgcggg ccggtcatga
     8641 tgccgcctgg caggcgatca tgacccatgg cggctttggc tatgccaagg agtatcacgt
     8701 cgagcggctg ttccgcgaag tgtcgatcac ccggctcgcg ccgatcaccg agcagctgat
     8761 tctgagcttc atcgccgaaa aggtgctcga cctgccgaag agctactgac gcgcgccgag
     8821 aagcgcgtca gaggttgcgg gagatcaatt cccgcatgat ctccgaggta ccgccataga
     8881 tccgcagcac gcgcgcgtcc gcaaatagcc ggcagatctc gtattcgcgc atgaagccgt
     8941 agccgccgaa gaattgcagg cactggtcga ccagccgtcc gtgcatttcg gtgacccaga
     9001 gttttgcggt cgacgcggca taggtcgtca gctcgccgcg gatatgctgg ctcaggcact
     9061 gatcgacata ggcccagccg acctgaagtt ccgacttgag gtccgccagc ttgaagcgcg
     9121 tgttctgata ggtcccgatc gcctgtccga atgccttgcg ctccaggacg taggccttgg
     9181 tgatgtcgaa tgccttctgg gcggacgcga tcgagtgaag tgcgatcgtc aaccgttcct
     9241 gcggcagctc gctcatcagg gccgccatcg cgccgccttc cgagcccagg aggttggtta
     9301 cgggcacgcg gatctggtcg aagaacagct ccgacgtatc ggaggagagg tggccgagct
     9361 tgtcgaggtt gcgaccatgg cgaaagccgg gccgggtggt ttcgacgagg atcaggctca
     9421 tgccgcggga tccgccgtcc gggttggttc gggtcaccgc gatgacaagg tcgcacatct
     9481 gcccgttgga gatgaaggtc ttctggccgg agatgacgta ctcatttccc tcgcgcacag
     9541 ctcgcgtgcg cacgccctga aggtcgctgc cggtgccggg ctcagttatg gcgatggcac
     9601 acacggtctc gcccgcgatc atgcgcggca gccatttctt cttctgctcc tccgtgccgt
     9661 agctcaggag ataaccgcag cagacgtcgt tgtgaaccga gaagtcggtc gccggacctg
     9721 cgaagcccga ataagcgagt tcttcgatga cgacggcgtt atggcggaaa tcgccgccga
     9781 tgccgccgta ctcttcggac acctgggggc agagcaggcc ggcctggccg gccgccggcc
     9841 acaatgcgcg atcaatgatc ccggcctttt cccattcctg aaagtgtggc gcgattttgg
     9901 acgcggcgaa gcgccggaca gtttcgcgca ggagggcgtg ctcttcgtcg aatactgggc
     9961 gattgggcag catatctgtc ggccataggt tgggagctag aaggcgacct tgtcgccacc
    10021 cttgagctga aggatttcac gtgcctcgtc gggacttgcg atctcgagcc cgaggccttc
    10081 aatgattttg cgtacctgcg tcacctgtgc cgcgttggtt tcggcgaggc ggcctgcgcc
    10141 gatccagagg ctgtcctcga gtccgacgcg gacgttgccg cccatcgccg ctgccatggc
    10201 ggcaaccgac atctgcgcac gaccgacacc aagcaccgac cagcggtagt tgtcgccgaa
    10261 tagccgatcc gccgtccgct tcatgtgcat gacgtcgtcc ggatgcgagc cgatgccgcc
    10321 aagcagtccg aagcaggtct ggataaagag gggagccttg accagccctt ccgtccagaa
    10381 atagtgcaga ttgtagagat ggctcgtgtc gtaacattcg aactcgtatc gggcgcccga
    10441 actgttcaac gtctccagcg cgtatcggat atccttaaac gagttgcgga agacgaggtc
    10501 gtgcgagccc tccagcatct gcggctccca gtcgtatttg aacgacttgt atcgcttcag
    10561 catcggaaac aggccgaagt tcatcgagcc catattgagg ctcgcgactt ccggcttcca
    10621 ggtcgccgcc ggacgcacgc gctcctcgac cgtcatgtag ggcgaaccac cggtcgtcag
    10681 gttcacgacg acgttggagc tctgcttgat tacgcgcagg aagggctcga aggcctccgg
    10741 cgactgatcc ggtcgtccat cgaccggatt tcgcgcgtgc aggtgcacga ttgcggcccc
    10801 cgccgccgcg gcgccgagtg cggactccgc gatctccgcc ggtgtcaccg gcaggtgcgg
    10861 tgacatcgtt ggcgtatgga tcgatcctgt gaccgcgcag gagatgatga ccttgtgggg
    10921 cgccatggcg gcctagcgcg cgaacttgtg gaagtccggg tcgcgcttct cgttgaaggc
    10981 cgcgacgccc tccttcgatt ccgccgtgtc gtagaacagc ttcaccgcgt ggagcgcgaa
    11041 gttgctgatg ccgcggatat tgtcggaatc ggcgttgaag gaacgcttcg cgagcgcgat
    11101 cgcggtcggc gagcgctggg cgagcttgtc ggtccagtcc ttcacggctg cgtcgagttg
    11161 cgccatcggc acgaccttgt tgacgagacc catctcgcgc gcctgttcag cggtatagcg
    11221 ctcgttcagg taccagattt cccgcgcctt cttgtcgccg acatgacggg caaggaaagc
    11281 cgttccccag ccggcatcca ccgagccgac cttcgggccg acctggccga actgggcgtg
    11341 atccgcggcg atcgtcagat cgcagagcgt tgcaagcacg ttgccgccgc cgattgcaaa
    11401 tccattgacc cgggctatca caggcttggg cacgtcgcgg atcaggccct ggatctcgtc
    11461 gatcggcagt ccgaccacgc cgcgaccgtc atattggccc tcatgagcgg attgatcgcc
    11521 gccggtgcag aaggctttct cgcctgcgcc ggtcagcacg atgcttgcga cattgcggtc
    11581 gttggcggcg agctggaagg cctggatgag ctcgtccagc gtccgcgcgc ggaaggcgtt
    11641 atagactttc ggccggttga tgatgatcca cgcagcgcgg tcgcggattt catagatgac
    11701 atcttcgaat tgcttgggat cggtcatggc atgtcctcgc gcaggttaga tcatgttcat
    11761 tccgccggag acggagatgg tttgcccggt gatgaatttt ccgtcgtcgg aggccagcat
    11821 cgcgacgatc ccaccgtaat cgtcgggctc gcccatgcgg cggagcggga tgcctctgag
    11881 catggcgtcc ttccatttca ccgcgtgctc gccctcgcca agcacagacg ccatcatcgg
    11941 cgtgttggtc gggccggggc agacggcgtt gagcaggacg ttcttgcgcg ccagctcgcg
    12001 ggccagcgac ttggtgaagg agatcagccc gcccttgcag gcggaataga cggcctcgtc
    12061 gctcgtgccg acgcgcgccg catcggaggc gatattgacg atccggccgc cgccgcgctc
    12121 ggccatcagg ggggcaatcg ccttgtgcgt gttcagtggg ccatagaggt tgatgcggat
    12181 gatcttgtcc cacagctctc tgtcggtctt caggaacggc atcggccggt cccagccggc
    12241 attattgacg aggagccaga tcggtccgag ctcggcctcg accctggcga ccgcggcgtt
    12301 gacggaatcg gcgtcgccga cgtcgacggc ataggtcttg atgacggcct gcggggcgag
    12361 ttttgcggtc tcttcgccgc cttgcggatt gatgtcgaag atcgcgatct tgcagccttc
    12421 ctcggccagc cgtagcgtca acccccgtcc aattccctgt ccgccgccgg tcacgacggc
    12481 aacttttccc gataggccct tcaacgctga tctcccttgt cgtttatagt tgtcttgcct
    12541 tgcaggcgat cttcgatgtt tttggagagg ttaggcttac gacgccgacc cgttcttctg
    12601 aaggtcaaat ccgcgctgcg aatacatctg tcgcgcgatg atcgttttca tgatctcgat
    12661 ggatccgccg gcgatcttga caactcgtgc atcggcatag gccctggcga tcggatattc
    12721 ccacatgtag ccccaaccgc cgaagagctg gagacattcg tcgacggtct cgcagtgcag
    12781 ttcggacgta aacatcttgg ccatggccgc atcgacggga tcgagcttgc cttccatgaa
    12841 cagcgcgatg cacttgtcgg tgaagacacg ggcaaccgtc gccttggtct tgagttcggc
    12901 gagcttgaat tgggtgttct ggaagtcggc gagcttctgg ccgaaggcgc tgcgttcgga
    12961 ggtgtattgc agcgtccagt cgatcacggt ctcggtgacg gtcgccgacc ggatggcctg
    13021 cgccagccgt tcctgcgata gtttggtcat catctgcgcg aagcccctgc cttccgcgcc
    13081 cagcatgttg ctggcgggaa tgcggacatt gtcgaagaat agctcggagg tatcttgcgc
    13141 cctcatgccg agcttttcca gatttcggcc acgcttgaag ccgtcacggc tactctcgac
    13201 aatgaatagc gtcacgccct tggcgccggc ctggctgtcg gtcttggttg cgagcacgat
    13261 gaagtcgcaa agctggccgt tggagatgaa gaccttctgg ccgttgatga cgaaatcgtc
    13321 gccatcacgc gtcgcgcgag tccggatcgc cttgaggtcg ctgccggcgt gaggttccgt
    13381 catgccgagc gagccgatcg cttcgcccga gaccatcttg ggcagccatt tgcgcttttg
    13441 cgcctcgctg ccgaacgaca ggagataggt ggcgaccaga tcggtatgga tgagaaagcc
    13501 ggggccgctg gcgcccgccc gccagagttc ctcgaacacg accacgtcga agagatagtc
    13561 gaggcccatg ccgccatatt cttcgggcac ggtgcagcac aggaggccgg ccgcgccggc
    13621 tttcaaccat agatctcgcg ggacgatgcc gtcttcttcc catttggcgt ggaacggtac
    13681 gatctcgcgc tcgacgaagc ggcggaccgt ctggcggaaa atctcgtgtt cctcgcgaaa
    13741 gatggtgcgc tcgatcattg tcaaaatacg tcccgtctga tttagtttgc tagtgtacag
    13801 tatttgattt gggtatacaa cccgaaacgc caaacaggca agtacgatcg tacaccaatg
    13861 aacagtagcg ggaagggaga ggggatcatg gcagtcgggc cggagctgaa gcagtttcgc
    13921 atcgagctat cgaaagagcg cgtcctgcac ctcatcttcg acatgcctgg acgatcgatg
    13981 aacgtgttct cgaatgcggc gatcgaggag cttggcctgt tcgcggactg gctcaagcat
    14041 agcgacgtgg cgggtgtcgt catccgatcc ggcaagtcat ccgccttctg cgcgggcgcc
    14101 gacctggccg agctggagac ggcctatgac atgatcatgg cggcgccgcc cggcgagcgc
    14161 gatcgccttg ccttcgatca tttctttcga ttgagccatg gcttgcgccg gctcgaggca
    14221 gccggcaagc cggttgcggt ggcgatcggt ggcctcgcgc tgggcggtgg ctgcgagttc
    14281 gcgcttgcgg cgcatcaccg ggtgattgcc gatcaccccc aggcgacatt cggcttgcct
    14341 gaatctctcg tcggattgct gccgggcggc ggtggaacac agcgtttgcc gcgtctgatc
    14401 gggatagagg cggcgctccc cgtgttgctc gaaggcgtgc ggctgggcgg acaggcggcc
    14461 cttgctgccg ggcttgcaca tgaattggtg gcgccgggcg aggaagttgc ggccgctgag
    14521 cgttgggtgc tgtcgcgacc gcaggctgcg cagccatggg atcagccgac ctggcgcgtc
    14581 gtggatgcgc aaggcgtgag cgcggccatc agagagaagc gaaacaagat gctggccgag
    14641 acgctcggcc actatcccgc tccgtcagcc attctcgact gtgtcgaaca gggactcccg
    14701 caagacttcg acaccgccat ccggaccgag atgcagatct tcgcaaagct gatccagagg
    14761 cgggagcccc gaaacatgat cagaacgttg ttcctcggac ggctcgatta tgagcggctg
    14821 aagaagaagg gcatgaatcc caagatcgcc gaggccgtcg ccgcggtgtc gcgggcgctc
    14881 ggcgttccgt cggagcgcgg caaggagctc tccgaggcgt tcgcgcgggc gggctttcct
    14941 gtcgatcagc cgcgcaagct cgagttcgac gggcacatcg caggcgccgt ctactggcac
    15001 gacggcgagc cgaggacctg gggaaaggag ctgttgcgcg cgcgattggc ggatgccgag
    15061 gctgcggccg cgagctggcg atcccagctt gatgagtcgg aacgccgggc tgccgactat
    15121 gtgctggtca cgcaggccgg ctttcccgcc tatctcggcg gcctgtttgc cgccgggctg
    15181 agctaatcag tgccctggag ggagaaaagc cgtgtccaga cctcttgaag gtatcaaggt
    15241 tctcgatctt tcgaaggtgc tcgcgggtcc gctctgcgcg caatatctcg gcgatctcgg
    15301 cgctgaggta attaaggtcg aagcagtggg gcagggggat gagacgcgcg gatggccgcc
    15361 gtttcccgcg cccggcctcg gtaccgtgtt tctgagcgcc aatcgcaaca agcgaagcat
    15421 cgccatcaac atgaagtccg acaagggccg gaagctggtg catgagctcg cgaagtccgc
    15481 tgatgttgca atcgagagct tcggcaccgg agttgccgag cggctgggaa tcgacgccgc
    15541 gagcctttgt gcgctcaacg accggctggt ccattgcagc atatccggct ttggccggac
    15601 gggaccgctg aagaactccc cgggctacga cgtgatcctg caggctttca gcggcatcat
    15661 gtccatgacg ggcgatgagg gcggcggcta tatccgcagc ccgatttcac cgatcgatca
    15721 gatgacgggt gtgcacgcct tcagcggcat ccttgccagc ctctttgcgc gtgagaggtc
    15781 gggcaggggc gcggcgatcc aggtgtcgct gttcgatacc gcgctgggac tgcttggcta
    15841 caacctccag accttctggg aacggggaac gcagccggcc aagtgcggct ccagccatga
    15901 gtcgctgtgc ccgtatcagg ctttcgagac cgcggacgga ccgatcatga tcggggtggc
    15961 caacgacaat ctctggcgca agttctgcgc ggtcgccggt ctgagtgcga tcgtggacga
    16021 cccgaaattc cgcaccaatg ccgagcgggt caagcaccgc gccgagacgc ttcaccacgt
    16081 gcaatcggtg ctcgcgacca agacggtcgc gtattggaac gcggtgctga acgaggtcgg
    16141 cattccctgt tcgccgatca acacgctcgc gcagcttctg gaccatccgc acaccagggc
    16201 cgacaagctc atcatgcatt acgatcatcc tgcggccgga agtttgaatt gcgtcggcca
    16261 ccccgtcacg tttgttggag cggagcgcag ccccggcctg ccgccgccca tgctaggaca
    16321 gcacaccgac gacgtgctca aggaattggg gctctccgcg gcgagcatcg ctgatctgcg
    16381 tcgtgaggag atcgtcagtt gattggcgcg ctggtcgccg cacgcaacgt ggcggtggct
    16441 cagatcgtag tgaagaaggg cagtggtgcg ccgtcggtcg gcttgaacac caccttcacc
    16501 ttctggccga tcttcagcgc cgtcagatcg caatcgacga agttggtctg cagcgacggg
    16561 ccctccttca gcgtgacata gccgatcgcg taaggcccgg tcggcgattt ccgcatcagg
    16621 ctgtaggtgt agatcgtcgc ctcgccggaa ctctcctccc attccgtctt atcggagaag
    16681 cagaacgggc agatcgagcg cgggaagtag tgcgcttcgc cgcaggccgt gcagcgcttg
    16741 atcatgaatt tgccctgttt ggccgcctcc cagaacggcg cggtctcggg gttcgtcacc
    16801 ggggccggat actttttaac ttcgctcatc acacgcgctc caggattgca gtcgaggcgg
    16861 cgtgacggac acccaagagg ccgccggtgc cgtgggcgat ggcgagatcg cagttcttca
    16921 cctgcacctt cggatgcgcc tcgccgcgca attgcctgac ggcctcgatg atcttggtca
    16981 tgccgccgcg gttgacggga tgattgctgc acaagccgcc gccgtcggtg ttgaacggca
    17041 gcttgccgac accggagatc agattgccgt cggcgacaaa cttgccgccc tcgcccttct
    17101 tgcagaagcc gaggtcctcg agctgcatca agaccgtgat ggtgaagctg tcgtaaatcg
    17161 aggcgtattt gatgtccttc ggcgtgatgc cggcctcatc gaaggcgcgc gggccggacc
    17221 acacgccggc ggagtaggtg aggtcgagat ccttaccgcc gcgcgggccc ttcatcgcct
    17281 cgccatggcc gatcagtcgc accaagggtt tcttcaagct cttggcgatc tccgggctgg
    17341 tcacgatcag cgcgccgccg ccatcggtga caacgcagca gtcgagcctg tgcagcgggt
    17401 cggaaatcat cggcgagttc acgacgtcct cgacggtcac gacgtccttc agcatcgcgt
    17461 gcggattgta ttgcgcgtgg tgggaggcgg cgaccttgat ccaggcgagc tgctcgctcg
    17521 tggtgccata gtcgtgcatg tggcgcatgg cgcacatgcc gtaggcattg tgggtcgtcg
    17581 cgccataggc ggtctcgaaa tcggcctcgg cgcccggggc ccgcacctgc ggcggaccgg
    17641 tgcgcggctt gcctgcaagg gtgatcagcg caatcgaaca tttgcccgcg gcgatcgctt
    17701 cggccgcgtg cccgagatgg atgatgtagg aacagccgcc ggtatccgtg ctgtcgacgt
    17761 gtcgcacctt caggccgaga taatcgacca tcggccaggc gccgccgggc gcgtcgcccg
    17821 aaaggaagta gccgtcgata tcgtccttgc tgagccccgc atcctcgatc gcccctttgg
    17881 cgacctcggc gtggagctgg gcggtggatt tgtccggcgc atgccgggtc gggtgttcat
    17941 agattccggc aatgtaggcc ttgcccttga tggtcaacgg agttctctcc cttagctggt
    18001 ccgagccggt gatgcctgcc ggcagcgccc ggcaggttgg ggcatgttgc cgttgctatt
    18061 cgtcgagcaa cttccaggac gtcttgtcga agatggccat gcgcaaggaa tggtaggggg
    18121 cgtagttctc cttggaattg gtcagctcga ttcccggaag aagcatgccg acgcgctcct
    18181 tgttgaaact cgttgcctgg gcaagcaggt tctcgcgcgt cagattgtcg ccgcatcgct
    18241 gcaggccctt tgcgatggct tgcgcattga tgtagccgga cagcgcaata aagtcgttcg
    18301 gactgtcgtt cggcgcccac ttcttcatga acgcgacgta atccttgacc tcggcgtctt
    18361 cggcccaggc aggatcgctg gcctgcttgg caaactgcgt cgtataggcg ccttggacat
    18421 tgtcgagtcc ggccggcgtc agcacgccct ggatggagtt gacggggctg gccagaaact
    18481 gaacgggttt ccagtttatc tcgttggcct tcttgatgcc ctgggccgca aatttgggtg
    18541 tcgtaaagta gaccagcgta tcggctcccg acgccttgag tttgagaatc tcggagtcga
    18601 tcgtcgggta gctgagctcg taggatgctt ccgctacgat ctttgccgta ccgtcagcgc
    18661 ccaggcccgc ctcgaggccg gtgaggtagt ccttaccgta gtcgtcgttc tggtagagga
    18721 cggcaatctt cgcgttcggc atcttcttga gaatgaattt gccaaagatg gtgccctcca
    18781 tttcgaacgg gggatagaac gggacggtcc acgggaagtt ctgagggtca ttgaagcggc
    18841 ggcctccggc cgagatgaag acctggggaa ccttgttctg gttgagatat ttttgaacgg
    18901 ccacattggg tactgtgccc acggtgccca cctcagccag aacgccgtca tcgtccacca
    18961 acttgcgggt ctgctcgatg gccttcggcg ggctgaaagc gttgtcgagc gatatcaggt
    19021 tgacctttct gccgttgatc ccgccggctt cattcagcat ctggaaatag ccgctcataa
    19081 cgcggccgta gctcgagaag gcggaggccg ggccgctata gggaacggtc tggccgatct
    19141 tgatctcggt gtcggtgacg cccgggccat attgtttttc ggcggcgccg gcttcgaaga
    19201 tggcgccggt taggaaaagg actgaagcgc tgaccagaat cgaagttctc atgctttctc
    19261 ccattcgttt cttgtgggcg tcggacgcag ctgacgatct taataagtac actactatac
    19321 catattttag aaaacgaaaa tgcgaagttt gacgggtatt gcctcatgga tttaccgcat
    19381 tgcggtgcgg gagatccatt cggcagcgtc tcctggtttc ggagcagcct tgccttgacg
    19441 tcacggtgat tgtggttcag atagtacgta agtgtacgaa atggccggtg agatgaccgg
    19501 cgcagggagg gaccatggct gcttcgggcg caacggcact ggccgcgctg aagatcggtg
    19561 gacgcgtcgc cttcgagcgc ctgatgaggc cgaaggcgcg gcgggcggat gacgttccat
    19621 gctcgcccga cgccattacg cccgaatggc tgacggccgt gctctgtcag aaggtccctg
    19681 gcgcggtcgt caccggggtc gtcgtcaagc cgtccagcgc ggggacgcat gaacggcatc
    19741 agttgatcat cagctacaac gaggctggtc ggcgtgcggg cttgcccggg tcgatcttca
    19801 cgaaatcgct gcccagcgtc gtgacgcgga tgatcggcgg atttaacggt acggcgcggg
    19861 tcgagggccg cttttacatg cagctccggc cgcagctcga gatcgaggcc ccgatcggct
    19921 atcacgccgc ctcagaccgc aagacgtttg cctcgatcct gctactggag gacatggtcg
    19981 caacgaagtc ggcgacgttc tgcaattacc ggacttatgt ctcacgcgag atggcagagg
    20041 acatgatcgg cgtccttgcc gcgcttcacg cccgcttcca tggcgacagc gagcttgcga
    20101 cccgctatcc ctgggttgcc agctatccgc gctggttcac gatcggcgcg gcgaagatga
    20161 gcctggaata ctacacccgc aaggccttcg acgccgccgc gcgcgtcatt cccgcaagcg
    20221 tgctggcgca gcgcgacgac gtgtggccag ccgccactcg tgctcttgcg atccacgaca
    20281 gtgagccgca ggggttgatc cactccgacg tgcatatcgg caattggtac cgcacggggg
    20341 caggcaagat gggcctgtgc gactggcaat gtcttgcccg tggtcattgg tcgcgggatt
    20401 ttgcctatgc cgtgactgcg gcgctgacac ccgacgaccg ccgcaaatgg gagaaggatc
    20461 tgctcaatcg ctatctcgac cggtttgcgc aactcacggg cgcccgtccg gatttcgaca
    20521 ccagcttcct gcgctaccgg cagcagatgg tgcacgccct cctgatgtgg acgatcacgt
    20581 tgtgtcactc gccgctctta cccgccatgc aatcgggaga gacgaccctg gccatgatcg
    20641 agcgcatctc gacggcgatg gccgacctgg atgcgcttcg cagctgaccg ggataagctg
    20701 cagcacctgg ggccgggctt caacgagcgg ccagggcaca ttctccgcga tcgagcacca
    20761 atactcggcg ctcgacggcc caggccctga accggatggt gctgccctct tcgaacatct
    20821 cgatgcggat cgtgtcgccc ggaaaggcgg gcgagacaaa tcgaaccgcc atggtgcgca
    20881 gtcgctcagg gtgtccggaa gcatagcgct tcagaacggc gcggcaggcg aggcccatgg
    20941 tgttcaagcc atgcgaaatc ggtcggtcga aacctgcctc gcgcgcgatg gccgggtcgg
    21001 cgtggatcgg catgtagtcc cggctcacga gacgatagag cagggccgct tgccgaggcg
    21061 tgcgataatc gatgctggcg ctgggttctg catcggattg aattcggatc atttcgccgg
    21121 gcggcatgcc atgactgccg cagccgccgt cgccgcgcag aaactcggtt gcgcgcaggc
    21181 tcgcgattcg ctcaccgcgc gtggcatcaa acagctccgt atcgaaatag aggagggcgc
    21241 cgcgcgccgt tcccttgtca tcgacggcgc tcacccgatg ctcggcacgg atcgatccgc
    21301 tcaccgccaa ggggcgatgc agcgtgaggc gttgctcgcc atgcagggcc ttggtccagg
    21361 cgatcccggt ctgcggttcg gcaatccaga gcggcggcca tccgagcgtg acgcccatgc
    21421 tgggcaccgc acgctggttg ccgccctcat acacgtaggt cagctcgtct tcgtccagcg
    21481 gatcatcgcc catgccgaga ccaagcgcat acagggctga atcgcgccag tcgtaggact
    21541 gatggatcgg ctcgaagcgg cgcgcgagga cgtgttcaag gttcatgcgg gtgctccctg
    21601 cgatgctcga ttgccgaagc cgagaaaggc tcggccgccg agcccgggaa agatcgttgc
    21661 ggtgcgtacg aagttcgtca agtcgctcga acgtgccggg cgcgtctcgc gcatgatgtc
    21721 gcaggtgcgt cgggccgatt aggatttgca gcgaacatca gtatatgata agccagtgta
    21781 ctaatcgggc attgaacccc gaaatcgtcc agcgggacgg ggaaatgctg aaggcggcgc
    21841 cgcgcgtgat ttgagtatcg gtcgcggtcg tcgagcgatg gcaggagggc cctttgcgag
    21901 ctcaagcgcg cggattggcg acgcctcgga tggtcgtatg ccgccgaggg atgcgtcgag
    21961 acagaagagc gctgccgtga tcgcgctgtt gatcgcgaat tcgctggcgc tcgcctcgct
    22021 gctgatcatg ctgtccagcg ggctcgccct gatctacggc cttcgcgacg tcatcaattt
    22081 cggccacggc gcaatctata tgctgggcgc ctatatcggc tacaccatcg cactcgtcgg
    22141 cggattctgg ctggcgttgt ttctcgttcc gatcctgctg gcgcttctcg gcattgtctt
    22201 cgaatatctg gcgctgcggc cgctgcagag acgaacgcac atcgaggtcg cgctggtgac
    22261 cctcggattg ggcattatcc tggggcagat catcatcttc ttctatggcg gtgaggcgcg
    22321 cagcgtggac gcgccgaaaa ttctgtccgg ctcgatcacc gtcctgaacc tgtcctatcc
    22381 cgtttatcgc ctctttctga tcgtcatggg tcttggctct tgcgcgctgc tcgcgctctg
    22441 gctgcgatgc acgccttccg gactgtacgt ccgcgcggtg agccaggatc cctcggtggc
    22501 gcgcatgatg gggatcaatg ccgatcgctt gagcttgctc gtgacgagtc tcagcacggc
    22561 gttcgccggc atcgccggcg tcctggctgg cccctatctg tcgatcgatc ccggcatgga
    22621 tatcaccatg atcgtgaatt gcctgatcat tgttgtgatc ggcggcaccg gcagcatcgg
    22681 cggcgcaatc gttgcggcgc tgctgtttgg cctggtccaa gtggcgggat cggtattcct
    22741 gccgaacctt gcgtcgctcg ttccgtacat cctgctgatg atcgtgctgc tgatcgctcc
    22801 gggcggcatc gggcgcggga gggtattgag ttgagcaacg gtacgatgac cggcgatttg
    22861 cagcggaccg cgacgggagc gcccggcggc cagctctcgc ggatttcact cgtgctttcc
    22921 ggcggagcga tcgcgctcgt cgctggcgcc ctggtgccga cctaccttgc gggcgacttc
    22981 gtgctccggc tcggtagcga gggattgctg ttgggcatgc ttgcgctcag cgtcgccttc
    23041 atgatgaacc aggcgggctt ggtggcgctt ggcgcagcca gcatctacgg cggggccggc
    23101 tacctgttcg cgatcggcat gagcgatggg ggtctgacac ccgcggtcgc tctatgtgtc
    23161 ggcttcctgg tcattgtcgc ttatgccgct gtcctgggcg ccttgatcgt gcggaccaat
    23221 ccgctcgcct tcatgatgct gacgctcgcc gccggcgaga tgatcagtca tgcggttttg
    23281 cttgaggggc tgcgcgacta caccggcggc gctgacgggc tcgtcgtccg gatgaatgga
    23341 tcgatcctcg gcatcgacgt tgccgagttt gccgatccgg ctcgcttctg gtcgctggcc
    23401 tggatcgtga cggtccttgt cgggctggcg ttttggagcc tcggcaggtc acgcctcggc
    23461 gcaattcttc gcgccgtgcg agagaacgaa gagcgcatgc ggttctccgg cttcaacaca
    23521 ttcgtccccc ggctggtcgc ctacgtgctc gtcaatgccg tggctgcgat cgcgggtttc
    23581 ctgcatgtgc tcaacgccgg ctttgtctcg ccggagagtc tggggctgtc ggtcagcacc
    23641 aatgcgctgg ttgccgcgct gatgggcggc attgcgggct cgctcgggcc gattctcgga
    23701 ggactcatct tctcttttgc acaggacgag ttcggggcac gcgggctcac gcagctcctg
    23761 accggcgttg ccatcgtgct cttcatcgtc gtgttcccgc gcggcgtcgt cggcggggta
    23821 tcgagcgcgc tgcgacggtt cggctcgcgt cgggagatgt cctgacatgc tcaagatcga
    23881 gaaactatcg gtgagctatg gttcgctgcg ggcgttgacg ggcgtcgatc tcgaggtggg
    23941 cggggacggt gtgtttcacg gcgtcattgg ccccaacggc gcgggcaagt ccacgctttt
    24001 ggatgcgatc tgcggtcgca ggcgcccgac ctcgggaatc gttcgttacc gcgatgaaga
    24061 catcacgcga cgctcggtca cctggcgtcg tcggcacggg atggcgcggt ccttccagcg
    24121 caccagcata tttcaggacc tgaccatacg agagcaactc gagctggttg cacagcatct
    24181 tggcgacgac aggctggatg acgtggtcga ggtcatggat ctcggcgcct atctcaacca
    24241 gagggccggg cgcatcgcct atggcgtgca gcgccgggtc gatgtggcgc tggccttgat
    24301 cggcagaccg cgcctgcttc tgatggacga gccgggggcg ggcctgtcgg ccgcagagac
    24361 gctgcgtctg ttccagcatg tgcgcgatct cgtgcgcgaa cgcggcatcg cggcgatcgt
    24421 ggtcgagcac gatgtcgacg cggtgttcgc gtgttgcagc acggtcaccg tgctcgatct
    24481 gggccggcat cttgcgacgg gaacacctga ggcgatccgg gctgatcatc gggttatcgc
    24541 ggcatacctg gggaccgctg catgagcgcg ttgcggatcc agtccctctt tgcaggttat
    24601 ggcgcgtcaa ccgtgctccg cggcatcgac cttaccgtcg aggagggcga atccgtcgcc
    24661 ttggtaggcc gcaacggagc gggcaagtcc accttgctgc tctccgtctt tggcgagacg
    24721 aagatccgtt ccggtgaaat ctgggtccgc ggccgtcgga tcgacaacct cccgggttac
    24781 acggcggcca agctcggaat ttcgattgcg ccacaggggc gtcgcatcct tccgaacctg
    24841 acggtcaagg agaacctgct gctgagccgg gcgacgggca ggtcggggta ctggaatctc
    24901 gacacgatct acgatctctt cccggtcctc gaggagcgcg ccgaccggct gggcaccatg
    24961 ttgagtggcg gccagcagca gatgctcgcg atcggccgcg cgttgatggc caatccaagt
    25021 ctcgtctttc tcgacgagcc ttctgaaggg ctgtctcccg tgctgatcga tgggctggtc
    25081 gagaccctca acaggattcg cggcgccggc accggcgtgc tcgttgtcga gcagcacctc
    25141 agcctggtga agcgcgcgac ggaccggttc ctcatcctgg ccaaaggcga ggtcgtcggg
    25201 tcgggcgcca ttggagtcat cggcacgccg gagaaccaag cgttgatggc cttgtaggaa
    25261 gaaacacgaa aagtggagga gggaagaaat gacaaagtca ttcattgatt ggggctctac
    25321 caggcttcgc gcggcgacga tcggcgccgc gatggcactt gccgtcacga ccggcgcgct
    25381 ggccgaggat acggtcaaga tcggcgtgct gttgatcgac agcgggccgc tcgcgggtct
    25441 caaggatacg caggtcaaag cggtcaacct cgcgatcgac cggatcaacg cggccggcgg
    25501 ggcggcgggt aggaaactgg aggcgatctt cattacctat cccggcacac ccgataccgc
    25561 cgtggatggt gcaacccgcg cggtccagaa ggacggggcg ctatttatca cgggaatgga
    25621 tacgtcggct gtcacgcctg cgctccaggc caagctgccc gcactcaaag tcttgatgtt
    25681 ggaggtgatg gccaatgccg acggtctgac tggaaagaac tgctcgccca actatttccg
    25741 ggtcaatgca aacgattcga tgatcatggg cactttcagg gagttcctga aggatcaggg
    25801 catcaagaag tgggatatcc tcgcggtcga ttacgcggcc ggacgcgact ccgcggagaa
    25861 attcaagggg ctggtgacat cgcaaggcgg gaccgtcggc aagaccctgt tttcgccgtc
    25921 gggcacgccg gatttcggcg ccaagatttc ggagctgggc gcggaccccg ccgacggcct
    25981 gttcgtgacg atcttcggca gcgatgccat caacctcgcc aagcagcagc agcaattcgg
    26041 tctcttcaag aaatacaaga tggtgctcgg caactcgttc gtgatccccc agactttgcc
    26101 ggcgcaagga gaaacagtgc tgggcgtgta tcaaaacatc ggtttcgtgc ccggtttccc
    26161 cggcgcgcag gctgaggcct tcgtgaaggc gtacaaggaa aagtacaacg gggagcttcc
    26221 gccctacacc agtgcggacc agtacggtgc gatcgagctc atggctgccg ccatccagaa
    26281 ggcaaattcg acggacatca acgcggtgcg cgcggctctt tcgggcctca aggctgagac
    26341 ggttctgggc gatgtcgagg tgcgcgcggc cgatcatcag accgcgcggc ggatggcgat
    26401 cagtcagatc gccatggggg ccgacggcaa gcctggctac cagatcaaga agattgagcc
    26461 gggagccgat atcattcctc cggtggatgc cgcctgcaag atgtgaccat tcggcgccgt
    26521 tttcgacccc gtgtcgatgc ggcgccgaac gccatggtga ggcatcaagt cttcggcaac
    26581 cggttcggcc cagctacgcc acgagcgaca ccttgccgaa ctgcgccccg gatgcaagcc
    26641 gggcgagggc cgagcggaca tcggtcattg catgggatga atcgatgaca ggcttgacca
    26701 gcccgcggtt gaacacgtcg agcaattgac ggaattcgct gatgctgccg ccggtcgagc
    26761 catagatctc gagctggcgg atgaatacgc gttgcaggtc cgccggcgga ttggagccgg
    26821 tcgtggcacc gcatgtgacg agccggccgc cacgacgcaa cgagcggagc gattcactcc
    26881 agcttgcctc gccgacgctg tcgatgacca tgtcgacgcc gccgttgctc atttcctgga
    26941 tggcggcaga gacccggtcg tgccggtagt tgaccccacc gtcagctccc aacgccaagg
    27001 ctttggcgat cttctcatcg ctggaggagg tgacgcagac gcgcgcgccc accattcttg
    27061 caagttggag gcaggcgacc gcgacgccgc cgccgatgcc gactatcagg acgctctcgc
    27121 cgggctggag cgcccgcttg ccgaaaagca tgcgccaggc cgtgagatgg cccgtcatca
    27181 aggcgccggc tccgacgaga tctgcgtcat cggggagagg gaagaagcag ttgctgggca
    27241 tcgcgatgta gtcggcaaag ccgccatggc ggtgttcgcc catgatcttg acgttctcgc
    27301 aaagcggctg atcgccggcc aggcagtagc gacatctgcc gcaataggcc tcagaaaaaa
    27361 ggacagcctt catgccgggc ctcagtccgc tgccgggagc cgcctcgacg atcgtgccag
    27421 cagcctcgac gcccatgacc tgcggcaagg tatgtgtgat ccccttccca ttgtcgcgca
    27481 tgtagaggtc gacgcggttg acggaggatg ctgcgacctt gagcacggac tcgtccggtg
    27541 cgggggccgg gtctgggaaa tcgcgccagc tcacgccgtc aatcccgcgc cgttccagaa
    27601 ccgctgcctt catcgcgacc tcccgtcggt tcggagacaa gtaatgcact agcacacgaa
    27661 actgagcgcg aggctattcg cgacttggca catatgtcac cagtttagtg ttgcagttca
    27721 tttcgaatat aatacactag cgcacgaaac tgccgtcaag cgcagcgaat gatcatcatg
    27781 gaggcaacgt catgagcgag actcgtttcg attccgcggc gcacgcgcgc gcgatgcgcg
    27841 cgagcggatt ttgggtcgac aagagcttcg acgagttcct gcagaagaca gtcgccgcga
    27901 cgcctgagaa actggcgctc atggcggagc gcgccgaccg caccgagcca aagcgcctca
    27961 cttacgcgga actcggcgat ctgatttcgc gggcagcagc cgcgcttcgc cgactggggg
    28021 tcggccccgc ggacgtcgta tcggtccagt tgccgaactg gtgggagttt gccgtgatcg
    28081 cgctggcggc cttccgggta ggggctgtcg tcaatccgct aatgccgatt tttcgtgagc
    28141 acgagctcac ctatatgctg ggcttcgccg agacgaagct cctcgtcgtt ccaaaactct
    28201 tccgcggctt cgatcatgag gcgatggtcc gcgcgctgca gccggggctg ccaaagctga
    28261 agcatattgt ggttgtcgac ggcgaggggc ctgcgagctt cgatcaggcg ctgctgtccg
    28321 gcaatgagcg ccttggtcca ccgccggtcg gtgacctcgg tgcgctccct gcggaccgga
    28381 tggcggtgtt gatgttcaca tcgggcacca ccggttcgcc gaaaggcgtg atgcattgcc
    28441 tcaacacgct gatgggatgc tgcatcggac ttgccggccg tttcggtctg gacgcaagcg
    28501 atacgatgct ggtctgctcc ccgctcggtc acatgaccgg gtttgcggca ggcatgctgc
    28561 tgggcctcaa gatcggcgcc accgtcatct ttcaggacgt ctgggagccg aagcggggcg
    28621 tatcgatcat ggccaaggag ggcgtcacct actcggcagg cgcggcaaca ttccttgcgg
    28681 acatgtgcga ggcagttgct gcgggcgcgg caaaaccggc ccagctgcgc aagtttctct
    28741 gcgccggtgc gccgatcccg ccggccttga tcgatcgcgc ctatcgcgag ctcgatctca
    28801 aggtttgctc gctgtggggc atgacggaat cgctggcgag cacgttgacc gaacccgagc
    28861 gtgcgctgga gaaatcgtcg aagaccgatg gacgtccgat cgaaggtgtc gcggtcaagg
    28921 tggttcgccc ggatggctct ccggccgcgg tcggagagag aggattgctc aaggtccgcg
    28981 gcgcgcagat gttcctgggc tactacaagc gggaggacat ggagccgttc gacgccgaag
    29041 ggtggttcga caccggcgac atggcttaca tggacgacga gggctacatc cggatcgacg
    29101 gtcgcatcaa ggacatcatc atccgcggtg gcgagaatgt tcccgttcaa gacatcgaaa
    29161 acctgttgtt caggcatccc tccgtgctca cggtggcgat cgtaggatat ccggatccgc
    29221 gactgggcga gcgcgcctgc gcttttgtgg tcgtgcggcc cggcgagaaa ctcgacctcg
    29281 caggtctgca ggcctatttg gcggagcata aggtcgccaa gcagtactgg ccggaacgca
    29341 acgaaatcgt tgccgagctg ccgaagacgc cggcgggcaa ggttcagaag taccaactcc
    29401 gcgaaatcgc caaggcattc tccgaaccga cacagaaggc cagcgcatga gcctgcgtct
    29461 gaaagatcgt gtctgcgtcg ttaccggcgc ggcacgcggg attggccttt cgattgccga
    29521 gcggttcggt gaggaaggcg ggcgccttgc atgcgtcgac gtcagcgcgc gtcgcctcga
    29581 gcccgcggtc ggtcaattga aggagaaggg atacgaggcg agaggctacg aggtcgacgt
    29641 cggcaagcgc gatgatgtcc atgcgctctt ttcggacatc gagcgagact tcgggtctcc
    29701 ggtggccgtg ctggtcaaca acgccgtatg ggcacggttc gagccgctcg ccgagatcga
    29761 cgtcggtgcg gtcgaacgga tgtttgccgt gggcgtgcat ggcctgatct ggaccacgca
    29821 ggctgcggcg ccgcagatgg agcggcgcgg cggaggttcg atcgtcaatc tgagctcggt
    29881 ctcggcgttt cacccggcca aggattcgat tgcctattct gccctcaagg cgggcgtcgt
    29941 cggtctctcg cgggcttccg cgatcgagct cggtgcgaaa aacatccgcg tcaacgtcat
    30001 cgcgccgggc atgatcggga cgcccgcctc gatcgcacag ttcgacgagg cgacgatcaa
    30061 ggcgaggcag tccgagatgc ccgccggacg gttcgggaga ccggaggaaa ttgccgcgct
    30121 ggcggcgttc cttgccagcg atgacgcgag ctacatccag ggcgccgtca tcacggcgga
    30181 cggcggctgg accgttcctg ccagatagga gaatgcgcat gaggcggctc gagggcaagc
    30241 gcgcggtcat caccggcggc ggaagtggca tcggccgggc cagcgccatt cgtttcgcac
    30301 gcgagggtgc cgctgtcctc gtggtcggcc gggccggcaa tgccgaggag acggctgcgg
    30361 cgatcagaag cgagggcggg gtggcgtctg ccctgatcac cgacgcctcc atcgaggaga
    30421 acgtcgcgag gatcgtatcg cactgcgtct cggagtttgg agggcttgag gtcttcttcg
    30481 ccaatatcgg cgtcaccggg acgaatacgc cgcttctgga gcagacggtc gaggagtggt
    30541 cggaggtcta ccgtatcaat acgatcagtt gctttctcgc cgtcaaatat gccggtcgtc
    30601 acatgacggc gaaaggctac ggctcgatca tcctgacctc gtcggccgcg tccttacgcg
    30661 ccaatgcggg tgcgatctcc tatagcgcga gtaaggccgc ggtgaacaat ctgacgcaga
    30721 cggcggcgaa tgcatttgcc gggactggag tccgcgtcaa cgcggtgctg ccggggctcg
    30781 tcgagacgca actgacccgc aaggtgttcg aggatgcccg cgcccgtgga gtcgagtcca
    30841 agcttggtca cgtgacaccg ctgaagcgcc ctggacgctc cgaggagatc gccgccgcgg
    30901 cggctttcct ggccagcgac gattcctcct acgtcgacgg tcaactgctc tccgtcgatg
    30961 gcggcgtcag cagcacccat ccctatggtc ggatagctct ctagcgagcc ggctgctcac
    31021 aacaagaaca acaggagagg cgccgtggat ttcgaactgc cccaggaaat cactgccaag
    31081 cttgccgagc tcgacgcgtt catcgaggct gagatcaagc cgctcgagcg cgacaacaag
    31141 cagttcttcg accaccgccg cgagaacgcc cgcaccgact gggacaatga cggccgtccc
    31201 cgcgacgaat ggcgtgcgct gatcagcgag atggagcgcc gcgccgacaa ggcgggacat
    31261 ctgcgctatg gcctgccgaa atcctgcggc gggcagggcg cgtcgaatct ggcgattgcg
    31321 gctatccgtg agcacctcgc ggccaagggc cttggtctgc acaatgacct ccaggatgag
    31381 tcctcggtcg tcggcaattt tccgataata cctgcgctgg ccgattacgg gacggcggaa
    31441 cagaaaacct atatcgaagg catcattacc ggcaagaaac acctctcctt cggtttgacc
    31501 gagcccggac acggcagtga cgcaacctgg ctggaaacct ccggcgttcg cgacggcaac
    31561 cattgggtca tcaacggccg caagcgctgg aacagccagg ttgcccgtgc gcacgcaaac
    31621 ctggtatttg ctcgcacatc ggggaagccg ggagaggcca agggcattac cgccttcatc
    31681 gtgccgaccg acacacccgg ccacaaggtt ctctacaatc actggacatt caacatgccc
    31741 agcgatcacg cggagacgga gcttctgaac gtgcgggtcc cggatagcgc catcctgcac
    31801 agggagggcg agggactggt cgtcgcgcag agatttgtgc acgaaaaccg catccggcag
    31861 gctgccgcca gcgccggcgc cgcgcgttac tgcattcagg aggcggtcaa atacgccaga
    31921 tcgcgcgttg ccttcggcga gcctctcgcc aagaagcagg cggtccagtt tccgctggtc
    31981 gaactctggg cagagtatca gatgctgcgc aactttatct ttcgcacggc atggcagatg
    32041 gatcgccaga atccgctgga gatcagcgac atggtgtcaa tttgtaactt ccgggccaac
    32101 cgtttgtgct gcgaggcggc cgatcgtgcg atgcaggtgc acggaggcat gggctatagc
    32161 cgcgcgctgc cgttcgagca catctatcgc catcaccgcc gttaccgcat aacggaggga
    32221 accgaggagg tccagatgcg gcgggtcgcg caatatctgt tcggtttcag cggcaagatc
    32281 gcgagctgag cgcgatgaat gtgcaggcga acgacgacag gctggatttc gaccttcgtc
    32341 ggctcgacgc atatctgaag gagtggctgg gcggatccgc cgccacccgc gtgacgcgta
    32401 ccagcggcgg aatgtcgaac ccgacctact tcgtgatgcg cggtgattgg cgtgctgtgc
    32461 tgcggaagca gccgaacaat gtcctgatgc cctccgcgca cgccattgat cgcgaattcc
    32521 gggtactcac agcccttcag gacagcgccg tccccacgcc gaggccttac cgctattgcg
    32581 aagaccgggc ggtcctcggg acgccgttct atctcatgga atggctggtg gggcgcgtcg
    32641 tttacgacta cgcgctgccg ggatttacgc gcgaggagcg cgccgcctgc tatcgctcca
    32701 tgtgcgcgac gctcgctgca atccacaagc tcgactacaa gaatgcggga ctcgatgatt
    32761 acggcaagcc gggaaattat tttgctcgcc agctcagccg ctggtccaaa ctgtgggaag
    32821 aatatcgcag gggcgatgat gacaacccgg ctctggacag agtggtgcgt tggctggccg
    32881 agcgcgttcc ggcaagcgag accgttgcac tctgtcatgg cgacttccgg atggcgaatg
    32941 tgatcttcca tccgaccgag ccgcgcgtga ccggtgtgct cgactgggaa ttgtcaactt
    33001 tgggtcatcc gcttgtcgat ctcgcgttca acagccaggc gtggcgcatg gcgccagatg
    33061 agaacggggg actcctcgga cttccgctcg atgaattagg gatcccgtgt gagcgggatt
    33121 atctcgacag atactatgaa gactcgggag cgaccgagcg cctgacggtt ttccatcagg
    33181 tgtttgcgat gttccgcggc gcggtcggaa gcgccggtgt ggcggcgcgc ggggatctcg
    33241 gcaataacgt gctgcctgat gcagcgaatg tagggcgacg gctcgcgcgg gcctatgcga
    33301 cgcgcgggat ggcattgatc gagagcgaag catgagcgca gcggagcggc ctttgatgaa
    33361 tgtcgatgag gcgatacggc tccggcgatc cgtgcgtggg ttccttccgc gcgaggtgcc
    33421 ggaggcgacc ttgcgggaag tcttcatgct tgcgcaatgc gcgccttcga actgcaacgt
    33481 gcagccgtgg aatcctcacg ttgtgtcggg cgagaagctc agacagcttc gagaggcgct
    33541 tgttgccgct gggatgcgcg atgagccgat caagccggac tggccggccg acggaaaatt
    33601 tgcgggcgtt tatcgcgaac gacaggtcgg tgccgcgcag gcgctctacg gcgccatggg
    33661 cgtggcgcgg agtgatgcgg ccggacgcag gctcgcctat atccgcaatc atgcgttctt
    33721 cgacgcgccg cacgccgtgt tcatcttcat gcccgagccg ttcgacacgc gagaggcgac
    33781 ggacattggc atgtatgcgc aaactctgat gctggcactg accgcgcgtg ggattggctc
    33841 ctgcgcgcag ggcgcgctcg gcctgtttcc cgacattgtc cgcgaacagc ttgagatccc
    33901 gggcaaccac aagctgctgt ttggcatctc gtttggctat gaggaccata gtgtcaaggc
    33961 caacgcggcg cgggtcgatc gggctcggat cgacgatatc gtgcgctttc accgctgact
    34021 cgctcggcaa atgtctcacc aagggagttc gtcttgaccg gacctctgca aggccttcgt
    34081 gtcgtcgaga tggggggcat cggacctgcg ccctttgcgg gcgcgctgct tggcgatctc
    34141 ggcgctgacg ttgtccgcat cgatcgcatc gccaaggccg gcgtcgagcc ggaactgcca
    34201 gcgcggttcg acttctacaa tcgcaacaag cgctcggtag cgctcgatct caagcggccg
    34261 caggcgatcg cgacggtatt gaaactcctg gcgcaggcgg acgcattgat cgaagggttc
    34321 cgtccttcgg tcatggagcg gctcgggctt ggcccggaaa cctgccaggc cgccaatccc
    34381 cgtctggtct atgcgcggat gacggggtgg gggcaggagg gcccgttggc gcaggaggcg
    34441 gggcacgaca tcaactacct cgcgcttacc ggagcgctgc attgcctcgg tgatgcggat
    34501 cggccgccac cgccgccgct caatcttgtt gccgacctcg gtggtggcgc cctctatctt
    34561 gtggtcggct tgctgtcggc cgtcatggag gcgcggcaat cggggcaggg acagatcgtc
    34621 gatgtcgcca tgatcgatgg ggtgacgcat ttgatgtcgg cattccaggc cttccggcag
    34681 cagggaagct ggacggcgcg gcggacagac aacatcgtcg atggcggcgc accgttctat
    34741 cgcacctatg cgaccaggga cggcaagttc gtcgctgtcg gagcgatcga gccgcatttc
    34801 tacgccaatc tgctgaaagt cttgggactt aacgccgaag acgtacccga tcagaacgac
    34861 cgttcggcat ggccccggat gcgggagcgt ttcgcgggca tcttcgccaa acaaacgcgc
    34921 gatgcgtggg tggccgctgc gatggggcaa gatgcctgcc tggcgccggt gctgacgatc
    34981 gacgaggcgc cgtcgcatcc ccaaatgaag atgcgaaacg catatgcaat gtttgacgga
    35041 gtgcggcacc cggagccggc cccgcgcttt tcccgcacgc gttccgaact ccaccgagcg
    35101 ccgccggcgc cagggcgtga cagcagggcc gcgctcactg attggggtat tccgccggac
    35161 gagatcgatg cgcttcaggc catcggtgcg atggcgggcc cctgagccgg ttgcccgcca
    35221 tcagcggcgt tgcgtcagtc ggcggagatg ggatcgccgg agatcgccca gctggtaccg
    35281 tcaaacttgg cgatgcggag aatcttgaac ggcgtgtagt ccccgggggc cgtgctgatc
    35341 gagacgccgg ggagcatcag gggcattcgg gtaccgtgaa gcgatgtcgc ctggcggatg
    35401 aggttgtccc gggtcagctc gtccctgcac ttcttcagca cggtggtgag ggcgttcaca
    35461 ttcatatagg cgaccaggac cgaatagtcg tcgggattgg ccgacggggc gtacttcttc
    35521 agaaagccct tgtatgcctt catctcctcg tcatcggccc aggcaggatc tcccggctgc
    35581 ttgaggaatt gcgtggtgac cagtcctttc gatgcgtcga gcccggccgg cttcagcact
    35641 gtttcgaccg atgccgtcga gccgccgatg atgtggagcg ggctccagtt gagctcgtgc
    35701 accttgcgga tcgactgagc ggcggctttc gccgacgact gctcgatcaa cgtatcgacg
    35761 ccggccgcct tgagttgaac gatctgggag tcgatcgtcg gatcggccag ctcgtaagag
    35821 gcctcggcga cgatctgcga tgcctttgcg ccaaggcccg cgcgcaatcc cttgacatag
    35881 tcccgaccga aatcgtcgtt ctggaacagg acgccaattt tcgcgtccgg cttggcattc
    35941 aggatgtatt ttgcgatgac ccgtccttcg gtctcgaagt cgggatagag cggcactgtc
    36001 cacgggaagg tcttcggatc gttgaagcga cgtccgccgg ccgtgatgaa gagctgcggg
    36061 acctgtttcg agttcagata tttctggatt gcgacgtttg ggaccgtgcc gatggtgccc
    36121 acctcggcga gtacgccgac gtcctctacg agcttgcgcg actgctcgac cgccttcggc
    36181 gcgctgtagg cgttgtcgag ctgcgtgaag ttgatcttgc gcccgttgat cccgccctgt
    36241 tcattgatca tgtcgaaata gccgactacg gcgcgcccgg cgccggcacc gaaggctgac
    36301 gccgggccgc tgagcggcgt cgattgccca agcttgatct cggtatcgct ggcccctggt
    36361 ccataggact tttgcgcgtg aagtggcgtc gaagcgagcg tcgctgacgc cgcaataatg
    36421 cagagagcgc ggccaaagtg cctgttcaag atgtttcctc ccatttttga ttgttttcag
    36481 ttcttatcgt agaagctcgc gggcgatcac catccgttga atctggttgg tgccctcgaa
    36541 gatctgcgtc agcttggcgt cgcgcatcat gcgctcgacg ggatagtcca tcgtgtagcc
    36601 atacccaccg aagatctgca ctgcgtcggt cgtgaccttc atggccatgt cgctgccgac
    36661 acatttggcc atgctggcaa ggatgttcag gcgcttccag tcgcccgcgt cgcctgcgcg
    36721 tgcacattcg tagacgagcg ctcgtgcggc ctcgatctgc atggccatgt cggccagcat
    36781 gaactggacg ccctggaact cggagatcga cttcttgaac tgcttgcgct ccttggcgta
    36841 ggccacgcaa acgtcgagcg cgccctgggc cagtccgacg gactgggcgc cgatggtggg
    36901 gcggttgagg tcgagcgcgc gcatggaggc ccggaaaccc ttgccttcct ctccgacgag
    36961 attttcaatc gggacccgga cgttgtcgaa gaaaatgggc gtgttcggtg cgccacgaaa
    37021 gcccatcttg cgctcgtgcc gcccaaaggt gattcccgcc atattcttcg gttcgacgat
    37081 gaaggcgctg ataccgtcgg cgccgggact gtcgctcgtc cgcgccatca cgacgatata
    37141 gtcggccgcc acgccgtagc tgcaccattg cttgcggccg ttgaggacgt agcaatcgcc
    37201 gtctttcctg gcgcgtgtgt tgatcgcggt cacgtcggag ccggcttgcg gctcggaaat
    37261 ggctatcgcg gttacgatgc cgcctttcgc gattatgggc aggtatttct tccgctgttc
    37321 ttcggagccg aagtgaagca gcggcatgat gaacatggtg ttcagcgcgg caatcgacgc
    37381 gcaggcaggg gagaacctcg agatctcctc ccgtgcgatg caggccatgg tcagattgcc
    37441 gttcggcccg ccatattgct cgggaaccca gagctgcatc agtcccatct cgccgaagag
    37501 cttcacgagc tccaggggaa agcgatcggt ctcgtcgatt tcggctgcga tcggcgccac
    37561 gtgcttggtg gccatcttcc tgacgttttc acgaaaggcg atctgctcct cggtgaagct
    37621 catggacgtc tccggtgggt ttttcgttgg gaggccaaaa aggggcttcc attgaatggg
    37681 tgatccattt aataattcac cgcatgtcga ccaagtcaag cccccgtaac tccactggct
    37741 cgcgcgttgc gtcgtccaaa cgtcgcgaac gtgccgccga tcaccgcacc gccggatcgc
    37801 cggccccggt ggcatttcgg ccgattcgca cccggcgcgc cttcgaggaa atttgcgatc
    37861 ggatccgcga gcagctcgcg ctcggggttc tcaagcccgg cgacaagctg ccgccggagc
    37921 gcgatctcgc acagcagctc ggcgtcagcc gcaacgttct gagagaagcg cttcgcagcc
    37981 tcgagatggc cggcgtgctc cggctgcaga agggcgtcaa aggcggtgcg ttcgtccagc
    38041 agggtgatac cagtcgcatg aacgtggtca tgcgggacat gctgagcctg ggaacgatct
    38101 cgatccgcga attgtcggaa gcgcgcgtgc aggttctcga cctcgtcgtg cgactcgcct
    38161 gcgtgaacgc ccgccagtcg gatttcgagg cgttgcaggc gaatgtcgaa cgaacggagc
    38221 tgtttacccg cgagggccgg ctgctcgagc gggtcgaatg cgcacgagag ttctacaaat
    38281 tgctcgcggt ggcctgcggc aacaaggtga ttgcgatgat caccgatgcg gtgaccgaga
    38341 tccacatgcg gtttgtctat gcgaaggtcg catcgagcgg ggttgcgatg gcgcggttgg
    38401 gcgagcggcg caggcaattc cttgcggcgc tgcgcgaacg aaatgccacc gcagcggcca
    38461 gattgatgcg gagtcacttg ggggctgttc agaagatgct cgagcaggat cccggggcca
    38521 tgtcccttca ggtcgctttg gcggaaatga agcctggcat gagacgatga gtgctcgttc
    38581 ggcaggaagc cgggtgtgaa taagaaaaac agcggaggaa gatgtgtccc gatacgccaa
    38641 atatgaatgc ctgactgtcg atgtggccga caaggtcgcg actgtgacct tgaatcggcc
    38701 gcaggcgcgc aatgcgatca atcagaagct gatccgtgag ctccgcacga tctgggacga
    38761 tcttgccgac gatcatgccg tcaacgtcgt ggtgctgacc ggcgccgggg agttcttcag
    38821 cgtcggcggt gatgtcaagg cgatgtccga gcggcccggc ggcgacgtgc tggaggaggg
    38881 cgaggtccac gaccccatga tcagccgccg cggggtgacc cgtcagctgg agctcgacaa
    38941 gcctatcatt gcggccatca acggcgatgc gatcggcctc gcagcgaccc atgcgctgct
    39001 ctgcgatatc accgtgatgg cggaagatgc gcgcatcggc gatacccatg tgtcgcgcgt
    39061 tggcctggta gccggcgacg gtggcacggt gatctggccg ctgctggtcg gtatcaacaa
    39121 ggccaaggag ttccttattc gcgggacgct cctgaaggga aaggaagccg agcggatcgg
    39181 cctcgtcaat catgttgcac cgcgagcgga ggtattggcg aaagcgcgcg agatcgcgat
    39241 cgagctcgcg aatggcccga cctgggcgat ccgctggacc aagctgtcga tcaatcagat
    39301 cgtcaaggac cgggtgaaca tgttgctcga ggcctcgatg gcgctcgaac aggtgacgtt
    39361 cgagaccgcc gaccacaagg aggcgacgat gtccttcaag gagaagcgca agcccaggtt
    39421 tggccagcgt tagccgccga tggatgcggc ggagcttccg ggcgacgaca tcagtgcgat
    39481 gctccgggaa tccctgcgcg gatttctcgg cgagcattgg agtgccgatt ccgtcaggcg
    39541 tggtccgtcg tcgcatgagg ttgcggcgat atggaccagg cttatcgggc agggccttgc
    39601 cactcttggc agcagtcgtg acgagggcgg gctccgcgag gtggcagtcg tgcttgcgga
    39661 gctcgggcgc gccgcgtgcc cggctccgat gtggtccgcg gcgcttgcca atttcgcgct
    39721 ctcgacctcg acgtgcgaaa gcgccgttga ttgcttgagc aagctgcacg caggctccct
    39781 gcggctggcc ttctcgttcg ggacactcga ccccgatgcg ggtgcaggcg cgattcgggt
    39841 caggggcgat catgcggatg ggctgcttcg gtttgttgaa gcggcgggga gttgcacgca
    39901 tctgctcgtc gcaattgacc gcgcggtcgt cgggctcgtg gcattggatg ccgcgggtgt
    39961 ggagatcgtc ccgacccgtg ccatgggcgc atgggtcctt catgaggtcc ggcttgatgc
    40021 ggtacccgtc acgatcatat cgctggatga tgcggtcctc gacgatctcc tcatcaaggg
    40081 aaagctggcg ctggcggcgc gggcgcatgg cgctgcccgg cgtgccttcg agctggtgac
    40141 ggactatgcc agggaacggc atcaatttgg ccagccgatc ggacgattcc aggcgatcca
    40201 gcataagctc gcgaacgggc tgattgtcct cgaaggcgtg cgcctgatcg tggatcatgc
    40261 ggcgaaactg cacgatcttg gtagtcgcga ctggcgctac ttcgccaatt gcgcggtcgc
    40321 cttcgcaagc gacgccttga ggcgagtgtc gctcgagacc catcatgcgt tcggcgccat
    40381 cggctacgcc gacgaacatg aggcgccgtt gcacttcaag cgggtgcacc tcgatacgat
    40441 cgcgctgggt ggagggccgc acgcgaagcg gtcgctggcc tcctcgttgc tggatgccgg
    40501 cagctcgggc ctgcctgaat acgatctcgg ggcagccggc aacgcgctac gcgagcaggt
    40561 gagggactgg ctggaacaac attggtcggg ggaccggaag gccgaattcg atcgcgagcc
    40621 gttcgccaaa cgggagttcg atgccgactt cgcgcgcgac atcggcaaga caggttggat
    40681 tggtctcggc tggtctcggg agttcggcgg ccaggcccgt tcgccgcttg agcagatcgc
    40741 gctcatggag acgatggagc ggggcgaggc gccgcgcatc ggtgccgcga tccaggccaa
    40801 tgccctgatg atgttcggga cgcccgaaca gcagcagcga tatctgcccg aaattcttcg
    40861 cggcgaagcc atgcacggga tgggctacag cgaacctcaa gcggggtcgg acctcgcggc
    40921 gctgcgcacc agcgcggtgc gcgacggcaa tcattgggtc atcaacggcc agaagatctg
    40981 gaccacgacg tggtggggca agtacatgtt cctggccgcg cgcacggaca aggacgccaa
    41041 gccaccgcac gccggcatca gcatgttcat cgtgcccatg aacgccgagg ggatcacggt
    41101 tcggccggcg accacgatgt atgacggctc cttcgccaac atcttctacg acaacgtccg
    41161 cattcccctg gagaacctcg tcggtgaggt caacgggggc tggaaggtcc tgaccggcgc
    41221 gctcgcattc gagcgtggac tggtcggcgg gggcatcgtc ctgaaggtcg ctcatgcctt
    41281 cgagcagctg cggcggcacg tcatggagaa ggacgctgcg ggaacggcgc tgagcgacga
    41341 tcccatcgtg cgcgacagga tggcgatgct ggccgccgag atcgagatcg ggcgtctatt
    41401 gatgatgcat tgcgccgagc tcgcggcagg cggcgtgacc ccgccggaat acggcgcgat
    41461 cagcaaagtc ttctcggggg agttgatgga gcgcttcggc gaggcggcgc tcgatatcct
    41521 cggcatgcgc gcggcgctat cggagcagat gcccggggcg atcgacaatg gccgtttcga
    41581 gcagaacctg cgtcattcgc tgatgtgggt gatcagcatc ggcaccaacg agatccagcg
    41641 cagcctcatc gcacaacggg cgcttgggct gcccagatag gagtgaccgc atgcaagagc
    41701 aggagactgc gaatctgccg ctcgagggtg tacgcgtgct cgacttctcg atcatgctgg
    41761 ccggtcccta ttgcgcgagg ctgctcgcgg acgccggcgc ggaggttatc aaaatcgagc
    41821 cgcccgacgg cgacgacatg cgtctgcggt ccccgttgcg gaacggccag agcgcctatt
    41881 tcggccagct caatgcgggc aagcgcagcc ttgcccttga cctgaaaagc gcagaggcga
    41941 tcgaactggt ccgccagctg gtcaaggaaa ccgatatcct ggtcgagaat ttccggccgg
    42001 gcgtgatgga ccggcttggc ctcggctacg acgcgctgcg ggcgatcaat ccgcgcctga
    42061 tctactgctc gatctcgggc tacgggcaaa cgggtctggc cgcggagcgc gccgcctacg
    42121 ccatgatcgt tcacgctgaa agcggctttg atcgctcgct gatgcggtat gcgggcgatc
    42181 gcgaccggcc ggcggcgggt gccgttttcg tcgcagacgt gctcggcggg atcttcggct
    42241 attcggcgat ccagaccgcc atggttcagc gcgcacgaac gggggtgggc cagcggatcg
    42301 atgtcgcctt gatggactgc atgctcaacc tgctcgtcta cgaattgcag gaagcgcaat
    42361 tcccgcccca ggcgccgcgg gccgcctatg gtccggtccg ggcttccgat ggcgacatcc
    42421 tgattgcgcc gatcacgccg cgaaacttcg ccgctctgtg cgaggtgacg gggcagggcg
    42481 agctcgcgaa cgacccgcgt ttcaggacgg tgccgggccg ggccgcgaac tggaccgcga
    42541 tgatgcaggt cgtcgagaaa tggaccgagc agcatacggt gcgcgagtgt atggctgccc
    42601 ttgataaggc tggcgttccc agtgccgaat atcgcgatcc gggagccgcc ttgaccgacc
    42661 cgcacctcct tcagcgaggc gcattcgcga cgattgccga tagcgcgggg gaattcctgg
    42721 gcgtcaatgc gccatggaaa atgtcggggg ggccgacggc catgcggcgg gagatccccg
    42781 cggtcggagc acaccgggac gatgtgctgt cccgagtcct ggggctctcc ggtgaagcca
    42841 tcgccagcct tgcagaagcg ggggtgttcg gaagccccaa cgcccggtcg aaaggttagt
    42901 tcgcatgagc gcgccggagg ccatccgtcc catgaaaatc attgctgcga tagcaaatgg
    42961 gccgcgcgat ccgttcgaga tcgggccctg cgagctctcg gcgccgcgag ccggggaagt
    43021 cctggtccgg gtgcacgcgt gcggcatctg tcacaccgat ctggcggtca agctcggaca
    43081 catcccggtg ccgctgccga aggttctggg ccatgaaggg gcgggcgtga tcgaagaagt
    43141 cggccccggc gtgacgtcct tccgacccgg cgaccatgtg ctgatgagct tcggctcgtg
    43201 cggtgagtgt ccgaattgtc gcgacggcgc gctcggctat cgtgaccatt ttcgtgccat
    43261 caatctgttc ggaaacaggc ccggcggctc ggcgctgagg cgtggcggga gcgagctggg
    43321 cggccatttc ttcgcgcaat ccgccttcgc aacccatgcg atcgccacca ccaggaacat
    43381 cgtcagggtc gatgccgatc tgccgctcga gcttcttgcg ccgctcggat gcggcatcca
    43441 gaccggcatg ggatcggtca tgaacgtgct tcggccgcag gccgggacct cgattgcggt
    43501 cttcggcgtc ggagcagtcg gcctttccgc catcatcgcg gcgcggatcg tcggctgctc
    43561 gacgatcatt gccgtcgacg tcgtaccgga gcggctgcgc atggcgcgag agctgggcgc
    43621 gacgcatgcg gtgtcaggcc gtgccgatga tccggtggcg gagatcgtcg aactggcacg
    43681 aggcggtgaa aatttttcgc tggatacgac aggcgtgccg gcggccgtgg caaactccat
    43741 caattgcctg cgtgcgagag gcaccagcgc gcaggttgcg gcgccgcctc gcgggaccct
    43801 ttacgcggcc gaagccagcg tcgtggtcgg ccggggaatc gccattcgcg gtgtcgtcga
    43861 gggcgatgcc aaccccgagc tgttcatccc gcgaatgatc ggcttcttcc gcgacgggcg
    43921 gttgccgctc gacaaattcg tcaggacata tccgctcgcg aggatcaacg agtctgttga
    43981 cgatctcgag agcggccgcg tggtcaagcc cgttctgacc atgcgggaga tcgggagcac
    44041 gacataaaga cataaacaag tagggaggag gacatgctgg atatcgcatt tgacaacgca
    44101 atcgccgatc acaggactta tgggagtgtc gacttgcagc accggctgtt tggtcgcttg
    44161 cggcgtgagg atccggtcca ttggacacag cccgacgggt atcggccgtt ctggaccatc
    44221 acgaaatatg ccgacatcat cgaaatcgag cgccagcctg atcgtttcat caacgcgccc
    44281 aggaccaagc ttctcagcat cgagttcgag agtaaagtga aggaggcgat gagcggcaag
    44341 ccgatgctgg ttcgggcgct gccgcagatg gataatccgg atcatgccaa gtatcagaag
    44401 ctgacccacg cctggtttca gccgcgccac attcgaacac tttcggagcg catggcggcg
    44461 ctcgcaaagg acagcgtcga ccggcttgtc gcgatgggga gcgcgtgcga cttctacaac
    44521 gacatcgcga tctattatcc gttgcgcgtc ctgatgacga tccttggact gccgcagagc
    44581 gatgaggagc ggctactgaa gatcacccag gcttatttcg gcggtgggga tccggagatg
    44641 cagaagggct ccgatctgat cgatgccacg attgcctatg ttgattattt caaggacgtt
    44701 gcacaggaga ggcgaagaaa tccgaaagac gacgtggcaa cggtgatcgc gacgtccgaa
    44761 atcgacgggc ggccgatcga agacttcgag gcctattcgt actgcattgc tctggcgagc
    44821 gcaggccacg atacgacgag cgccaccatc gccggtggtt tgctcgcctt gatcgaaaat
    44881 ccggattgca tggcccgcct tcagcgtcag cccgatttgt tgcccgaggc ggcggaggaa
    44941 atggtgcgtt gggtgtcacc cgtcaagcac ttcttccgta cggccgcggc cgactacgaa
    45001 cttcgcggca agacgatcag ggccggggac agcctgctca tcagctatcc ctcgggcaat
    45061 cgcgacgagg aagctttcga tcgccccttc gaattcattc ccgaccgctc gcccaatcgt
    45121 catctcggtt acgggtttgg cattcatgcg tgcatcggca ggcaccttgc caaggcggag
    45181 gtcgtggcct tcttccgcga actgctgagc agggtcgagc ggatcgaact cgctggcgag
    45241 ccggcctgga ccgaaacttc cttcctcggc ggagtgaagc ggctgccagt gcgctattcg
    45301 gcacggtagg aggcctcgga cgatcgcggc gcgtgcgctc ccgacggcaa cgaggtcagg
    45361 tttttgagga cgggggcggc gcggccgcgg cttttgcgat cgccagcatg cacttgagaa
    45421 agtgcgcgcg atcctccttg ctcaagggcg caagcaagga ggtctgaagt tcggcggcgg
    45481 cgcgcacgac gcccagaagg gcctcttcgc ctttggcggt gatctgaacc tggatcgacc
    45541 ggcggtcttc cgagttgttt cttttcgaga tcagcttcct tgccgtcatt cttttgagca
    45601 tctcgctcaa cgtattgcga tcgctgctga tgcgctccgc gagggcggac ggcgtgaggg
    45661 gaccgtcctg atagagtgcg atcaacacgc cgaactgccg cggcgtgatg tcgctctggc
    45721 ccgtcaggtc ctgatagagc acgtcatagc gggcttcgag caatcgaagc agaaatccga
    45781 ttccgccttc aaggcgccag ttgcgcgcga tatccgccgc gctcgcactt gttcctttgg
    45841 gggctgacat gcggtcgttg acccgaattg tcgaaaagca cctaggcgtt agcagcttgg
    45901 ccgatagctc tcaaccccgc tccgattggc gtccgggcaa gccgcctacc tgggtcgccg
    45961 acgggccgta atttgctatt gcaaatcgtt cgcttagata gtacgtatac gtatcattag
    46021 agggcgtgag ggaaatggcg tcgccgggga ggactgcttg agtctgcgcg agctgttgtc
    46081 gattccctac cttctcgaag ccgaggcggt cgaaaccgag cccggccaat ggctcgtgcg
    46141 actgtcctac cctgaactgc ccggctgcat ctcggaaggc gtcgtcatcg aggatgccct
    46201 gaaggaattg gagcggcgcc ggatcgaaac gatcgtggat ctcgtggaga cgggacggga
    46261 accgccggtg ccgcggaagc cgttggctgc ggccgatccg ctttggaccg caaatgatct
    46321 cggactgtca ggccgcgtta cagcgctctt gggccgtacg cccgggcagg aggcgtgatg
    46381 gcgcgcaatt caaaggagga aactcatgct ctcacgtgaa gacaatgaac ggttagtgag
    46441 ggtggggaag ggtactccgc tgggagctct ctttcgtctg tactggattc ccttcctgcc
    46501 ctcggcagac cttgtcaatg acgggcagcc gaagcgtatc cggctgctgg gcgaggacct
    46561 cgtcgccttc agggattccg aggggcgtgt cgggctggtc gatcaggcct gtccgcatcg
    46621 cggcgcaccg atggtcttcg cgaggaacga ggactgtggc ctgcgatgcg tctaccacgg
    46681 ctggaaattc gacgtcacgg gcgccacgac ggatatgccg gccgaaccgg tccgcagccg
    46741 gctcaaggag cgcgtgcgga tcaaggccta tccgtgccgc gagcgcaacg ggatgatctg
    46801 gacttacatg ggccccgatc aggctgatct tccgccgttg ccgaatgtcg aatggaatct
    46861 ggtcccgcag gagcaggtgc atatctccct gcgcgtccag gagtgcaact ggcttcaggc
    46921 ggtggagggc gagatcgatt ccgcccacgc gccgttgctc cacggtcgat tggattcgca
    46981 gggcacgacg agtgcctgga tccagaagcg cgatcttcgc ccgaccttcg aatgcatcaa
    47041 acaggacttc gggatgagca tcgcggcgcg gcgcaactat gacgccaaca cgctgtactg
    47101 gcgcgtcaat cagttcctgt tgcctttcta tacgctcgtg ccgccgttga tgccctttcc
    47161 ggatctcagc gggcatgcct gggtgccgat cgacgacgag aatacgcttt gcatcatgtt
    47221 ctcgtatcat ccgtcgcagc cgctgctgcc gaagacgcgg caagtcttct cggaaggaca
    47281 taatgggcgc gagagcgggc acgccagccg ccacgcgctc gtgcagcacc cggtgacggt
    47341 cccctatgcg ggctactgga cgaaatacca tccgcagaac ggcttccagt tcgactacga
    47401 ggcgcagaag accacctggt tctcaggctt gccgggcctc tgggtgcagg acggcgcctg
    47461 ccagagcggt gtgtcgccga tcttcgatcg taccagggag aacctctgct cgagcgatac
    47521 gggaatcgcg atgacccggc gcttcatcct ggaagcgctc ggagcctatc gcgatcacgg
    47581 cacgaagccg aagggcgtga ccgatcccga cgtgttcatg gtgcgcgccg tgtcgatgcg
    47641 gctgccgccg gaagagtcat gggccgacgt cggcgcaccc ttcatgcggg ccagactggg
    47701 cgcggatttc gggtacgaga tgtgagtgct tcccaggcca ttcacacgga ggcgcgctca
    47761 tgagcgaggg cttgatcgac tttcgcgtca agcgcatcag ctttgaggtg gatgggatca
    47821 attcctacga attgatctcg ccgacgggcc gcgatctcga tcccttcacg gctggagggc
    47881 atatcgacct ccatctgccg aacggcatga tccgaagcta ttcgctggtc aacgatcagc
    47941 gtgagcgcca ccgctacgtc atcgcgatca acagagagcc ggcggggcgg ggcggctcgc
    48001 gcttcctcca cgacaatctc agggtgggcg acatcgtcag cgtgtcacga ccgcgcaaca
    48061 actttgcgct ttgcgaacag gccgagcatt cgatcctgat cgcgggcggg atcggaatca
    48121 cgccgttgct gtccatggcc cgccgcctcg acgcgctggg gcgctcttgc gagttgttct
    48181 atgcggccag ggcgcggccc gccgcggcat ttctcgacga aatcgaggtg ctcggttcaa
    48241 gtgggtgcct gaaggtgcat ctcgactttg acgacgagcg atcggggcgc ttgttcgatc
    48301 tgtcggccat cgtcgagcgt gcgcccgcgc gggctcacct ctactgctgt ggcccgcagc
    48361 cgatgcttga agcattcgag gcggcggcga aggatcgccc ggctggtcaa gtgcacgtgg
    48421 aatacttcca ggccagggaa gcccccgcaa ttgaaggcgg tttcgatgtg agactcgcgc
    48481 gcagcaaccg gaccatcgcg gtcaaagccg gcaagacaat acttgatgcg ttgctggatg
    48541 cagggatcgc cgcaaaccac gcctgctcgg agggcgtctg cggcacctgc gagacgcgcg
    48601 tgctcgaggg cattcctgac catcgggatc agtttctcag caaggacgag caggcatcga
    48661 acaagacgat gatgatctgc tgctcgggtg cgaggtccgg cacgctggtg ctggatctct
    48721 gaaagcaaga atgtcgtcaa cagggtggaa tgtcatgaaa gtcgctttac gcattctggg
    48781 aatgtctttc ctcatcgcca gcgcgtgccc gctagcgttg gcggatgcag cacggacgat
    48841 gaagatcggc attctcaatg atgcttccgg cccatattcg gacaacgcgg gtgagggctc
    48901 ggtcacggcg gcaaggatgg cggccgagga cttcatgcag cagcatcccg acttcaaggt
    48961 cgagatcatc tcggcggatc accagaacaa ggcggatgtc ggggcggcca tcgcccgccg
    49021 ctggatcgag caggagaagg tcgatgccgt tgccgacgtg ccgaactccg cggttggtct
    49081 tgccgtcaac gaagtggttc gcggcagcaa ggcctcgttg atcgcctcca gcgcagcttc
    49141 atccgatctg acggggaagt actgttcgcc caacacgatc cagtggacgt tcgacacctg
    49201 ggcggcgtcg cacaccattg gcaattacct cgtccgtcat ggcggcaaga aatggtactt
    49261 catcgccacg gacaatgcgc tcggcaaatc catggtgcgg gacggcacgt cggtgatcag
    49321 tgcgggcggc ggcaccgtgc tcggatcggt caatgtgccc atcaacaacg ccgattacgc
    49381 ctcgttcatc cttcaggcgg atgcttccgg tgccgacgtg atcgcgttcg cgaccgccgg
    49441 cggcgacacc gtcagcctga tcaaacagtc cgcggagttc ggcttgaagc agaacggcag
    49501 gaccttcgcc gcgctgctga cgacgatgaa tgatgtcgag gcgagtgggc ttgccgtggg
    49561 gcaggggctc atcatcaccc agccgttcta ttgggacctg aatgatgcga gccggacctg
    49621 gtcggcgaaa ttcagtgcgc gccaacatga tcagtcccca acggcctttc atgccggggt
    49681 ctactcctcg gtgctcgctt atctgaatgc ggcgctggcg gcgggtacca acgatgccca
    49741 ggctgtcatt cgcaagatga aggagcggcc gatcgatgat gcgctcttcg gcccggtcgt
    49801 cgtgcgggcg gacggccgcg ccatccacaa tatgtacatt ctgaaagtga aggattccaa
    49861 ggcctcgaaa ggcaagtggg atcttctcga gaatgtcggt gtcttgagcg gacccgaggc
    49921 gtttcgtccc atcgaccagg gcggatgcaa gatggttgag caatcgaagg gcaattgacg
    49981 ctggtcgcgg tcatatcccg agaaggctgt cgcgcaaaca atggatgcac catgctggac
    50041 aacatcgaag ccgactttcc cgagcacacg ccgcgcccat caaacgcgcc gaaggcgctc
    50101 gaggggatac gggtcgtcga tttctcccac ttcatcgcag ggccgtttgc gacgatgatc
    50161 ctggctgata tgggggccga ggtcgtcaag atcgaggcgc ccggccgcgg cgacgatctc
    50221 cgtcgctatc cgccggtgca tcccgagctc aagcacggcg cgcccttcgt ctggaccaac
    50281 cgcaacaagc agagtgtcgc tcttgatctc aaatcgccgg aaggcgttgc ggtcgtacgg
    50341 gacttgatcg cgactgcaga tgtcgtcgtc gagaatttct cgaccggcgt catggagcgg
    50401 ttcgggctag actatgaggt ctgccgcaag atcaaatccg atatcatcta ctgctcggtg
    50461 tcggcttatg gacgcgatgg ggcgtttgcg gaccggctcg gtttcgatcc gatcgcacaa
    50521 gtcgaaagcg gttttgtgtc catgaacggt tatgccgatc gcgaaggggt gcgggcactg
    50581 tcgcccgtca tggacatcag cacggcgatg atggcatgca acgcgattct gggcgcgctc
    50641 gttgcccgtg agcgtagcgg ccttggccag gcgatcgagg tctcgctgtt cgacaatgcc
    50701 gtgctgatga cgggctatgc cacgatgcag catctgttca gtggcgcgaa tccgcagcgc
    50761 catggaaaca ccagtccgga tacctgcccg tcgggtgtgt ttcaggcgca ggattgtacc
    50821 ttctacataa attgcggcaa cgacaagatc ttccagcgcc tgatgtcgca ggtgatcgat
    50881 cgcgccgatc tcgcctcagc cgagatctac gccaccggac ccgaccgtat ccggcgccgg
    50941 gacgagctgt tcgcggtcct cggcgaagca ttcgcacacc agccctggtt gcactggcag
    51001 gcgcgcatgc gcgcagccgg cgttccctgt gggcaggtac ggagcgtcgg ggaggccatt
    51061 cggtcggccg aagcgcggga gcgtggtctg gtcacccgga ttccgcacga gctgttgggc
    51121 tggatcccca acgtcaactt gccgatccga tattcgcgca cgccgatcgt ggatccagtc
    51181 gcggcacccg cggtgggaca gcacaccgaa agggttctac gcgagcagct gggctacgac
    51241 gatgcacgtc ttgcacgact cgccgaggca ggcgcgtttg gcgacgcggc gagatcggcg
    51301 cgaggggacg tcaagatatg aattcgcgga cgctggccga tcgcacgctc agggggcgtg
    51361 tcgcggttat cgggatcggc gaaacggact attatcgcca tggcggatcg ccggacccgg
    51421 aattcaagct ggccctgaag gcgattctgg cggcctgcgg cgatgcgggg atcgatccgc
    51481 gcgacatcga tagtttctcc tcctatagcg atgaccggaa cgaggcgtcc aggctcgcag
    51541 cggcactcgg cacacgaagg cttcgcgccg cgaccatgca atggggcgga ggcggcgggg
    51601 gcagttgtgc ggccgtcgcc aatgcggcgg cgtcgatcgt tgcggggctc gcggactgtg
    51661 tggttgtgtt tcgttcgctc gcgcagggcc aatatggccg gttcgggcag acgaccggga
    51721 tcgacacggt ctccggtgag aaagcctacc tcatgcctta cggcgtgttg gcgccgccgc
    51781 aacgctttgc cgtgcgcgtg cgtcgctaca tgcatgagca cggtgtcaag caggaggcgc
    51841 tgcgtgcgat cgcgctggct tcctaccacc atgcgcagtc caatccgcgc gcggtcatgt
    51901 atggcaagcc gctcgatgca gcgaaatatg atgcgtcccg ctggatcgcc gaaccgtttc
    51961 atctttacga ctgctgcatg gagaatgacg gtgccgccgc gctcgtgctt gtgccggtgg
    52021 aacgcgccaa ggagtttcga agcaaggcgg tctatgtgct cggcgcggcc gtcggatccg
    52081 ggtatcgcac gggggccatt ccccacaact cgccgctcta cgcgagcgcg agcttcgata
    52141 cggtcgcgcc cgacctctac gggatggctg gtctcggccc atccgatgtc ggcgtcgtcc
    52201 aatgctacga gaacttcacg ggcggtgtgg cgatggcgct ctcggagcac ggcttcttca
    52261 agccggagga ggctaaccaa tttctcgcgt tcgagaacct cgttgctccc ttcgggcggc
    52321 ttccactcaa caccagcggc ggcaatttgg ccgaatgcta catccacggc ttcgagctcg
    52381 tgctggaggc cgtgcgccag gtgcgtggca cctccacgaa ccaggtcaag agaaacgacg
    52441 tggccatggt catcggcgcc ccgatggtga cgccggtgag caatctgctt ctcggctcgg
    52501 aggcgacttt atgagcgaaa agacgggcct gacctatctt ccgaccggcc tgccaattcc
    52561 gatagcggag gccgatggct tgtcggcgcc ctactgggag gggttacgcc agaatcgtct
    52621 cctggtgcag cggtgccgcc attgcgacac gtggcagttc gggccggaat ggatctgcca
    52681 tcgttgccac gcctttgatc ctcaatggac ggaggttgaa ccgcgcgggc taatcttcag
    52741 ttgggagcgg gtctggcacc cctcgcatgc ggccctgagg cagcatggtc catatctcgc
    52801 cgtgctggtg gagatcccgc atgcgggcgg cgtcaggatg gtgggcaatc tcctaggcga
    52861 ccctgtgcag atagtatcca tcggagcaag cgtgagggga atttttgaac accattctga
    52921 atctgcccct ccgtactcgc tactgcaatg gcaaacgagc tgatcggtcg gtcagcagga
    52981 tctgccaagg aggtgtcgag ttaatgaatc ggcctggtgg cgcagacccg ccttcttctt
    53041 gatcaggctg gatctatttt gctgtaggtg gctcaaactg gcccatgatg gattggagcg
    53101 agagttcagt tttctcaatc agttagaagg cggcgtgtgc acgatgtgtg cagcgagaaa
    53161 tcaaagaaaa tctagagata accatcgtga gcgggttgga ctaagcgcgt tgcagtgccg
    53221 gcggcggatc cgattcttcg gtagggagtg atctcattcg gcaagttgcg aatttgcgcc
    53281 gatcaaatgc cgaggattct gtcatctcga tcttcttacg ctcgcgccgc gattgctcga
    53341 agccttcggt gccggcgaat gaccgggcga cgtctcgggc gtgctcatgg atgtcgcgcg
    53401 gtaccttgcg tgatggttct ttcgggcaac actgcggttt cgctttcacg agactctaca
    53461 atcacattgt agcacggatt ccgaatcgat atctatcttg gctagaccat ctcgctcagc
    53521 actaactgcc ggatcaggca catgtcactc cgccgcttcc tccaaaggga gggatcgcga
    53581 aggtcgacaa acaactaggt ttttgcgatc ccagcgatcg agctcctcca acggatagag
    53641 aacagccttg ccgatcttga tgaacgatgg accgatcctc atcgaacgcc agttgcggag
    53701 cgtgccttcg ctgatctcgt cgcgataacg ttcgacaacc tcattgagcg tcaggaacat
    53761 cgggtcggac atgtctatct tccttgttgt tcagacactt ccggaaaggc gagcgactgc
    53821 gttcagcagt ctcgccctat ctgcgacgga ggtccgggat tgaatgagac cccgtccgat
    53881 accgagcacg agaattcaat ccacaaatgt ctgatgttga gggaagaaga gaccgaacgt
    53941 cgatgttagg gcgaccagct cgcccgcttt gatgaccaca ttgtcgctcg ccagcaccag
    54001 gccgcgggcc gcctacgaag ccgccgacaa cgcataggac gcagcagtga tggcgatcaa
    54061 tcgccggagc gcgggaaaga cagtgatccc tcaagcaaga agccgaactc tccgcccacc
    54121 cgtcagaggg cctcgacgat cgtcacattg gcaatgccgc cgccctcaca catagtctgc
    54181 aacccgtagc gcttacttcg cttgtgcagc gcgtggacga gcgtggccat gagcttggcg
    54241 ccggaggcac cgagcggatg gccgagagcg atcgcgccgc catttacgtt gagccgggcg
    54301 ggatccgcgc ctatttccct tgtccaggcg agcgccacgg acgcgaaggc ctcattcact
    54361 tcatagaggt cgatgtcatc gatcgagagg cccgcgcgat tgagtgcgcg tcgcgtcgcg
    54421 cggatcggtt cctccagcat gatgatggga tcaccggcag tcacggtcag gttgacgatg
    54481 cgggccagcg gcgttaggcc atgtgccttg agcgcggctt cgttgacgac cagcacggca
    54541 gccgtgccgt cgcagatctg gctggagttg gcggcggtga tgaccccgcg gtcctggagg
    54601 gttcgcaccg aaccgatcgc ttccagggag gcatcgcggc gaacgccctc atcatgcaca
    54661 tggacatgct cgttgcctgc cgcgtccttc accgcaatgg gaacgatctc gtcatcgaaa
    54721 gccccggcat cgatagccgc cacggcacgc cggtggcttt caagcgcgaa ccggtcgagc
    54781 gtttcgcggt cgaatccata tttggccgcc atcatttgcg cgccggtgaa ctggctgaac
    54841 tcggcgacgc cgaaccgctg ccggatatga tcggcaaagg gcgtacccat acccgcatcg
    54901 gcggcgagct tcgtcgccga gaacatggga acgcgggtca tgctttcgat cccggcggcg
    54961 atgactacgt cctgcgtgcc cgacatgacc gcctgcgctg cgaattggat cgcctgctgc
    55021 gaggagccgc actgtcggtc gatcgtcacg gccggaaccg tctccggcag gcgtgaggcg
    55081 agaacggcat gacggccgac atggaagctc tgttccccga cctggctcac gcaccccatg
    55141 atgacgtcgt cgatcgcgct ggggtcgatt ccagtgcgat cgagggtggc attcaggact
    55201 tcggcggcca gctcgacggg atgccatccc gcaagcgccc cgtttctgcg gcctccggcg
    55261 gtgcgtgccg ccgatacgat ataagctgcg gtcatggttg gaatctccga tttccagatt
    55321 cgtatgtagg ggatatgcgc gtcacttgcc ggcccacgcg cctttgcgct tctcgacgaa
    55381 cgcggccatg ccttcccgct tgtccgcagt agcacacagg ccgttgaata gccgccgctc
    55441 gaacagcaga ccggcggaca agccgacttc aaaggccgtg ttgaccatct ccttgttggc
    55501 aatcgtggcg agcggcggca ttgcggcgat ctcggcagcc gccttgatag cgtcctcgac
    55561 cagcgtcgcc gccggagtga cacgcgatac caggccagcg cgttcggcct cgtggacgtc
    55621 catcatcctg ccggacaggc acatgtccat cgccttggcc ttccccacgg ccttcgccag
    55681 ccgctgtgag ccgcccattc cgggtgcgac gccgagcttg acctccggtt ggccgaactt
    55741 cgcggtttca gccgcgataa tgaaatccgc catcatcgcc agttcgcaac cgccgcccaa
    55801 ggcgaaccct gccactgccg cgatccaggg cttgcgcgtg gccgtcaccc ggtcgaagcc
    55861 accgaaataa ttttccccga acatctccga gaaaccgcga ctggacatct ccttgatgtc
    55921 ggcgccggcg gcgaacgcct tgtcgttgcc cgtcacgacc gcacaacgcc ggctgtcatc
    55981 ggcctcatag gtcgcaaagg cgtcgatcag gtccgtcagc acctggctat tcagggcgtt
    56041 gagcgcttca ggacggttga ggcgtatcag cgtgacggcg ccatgcctct cgacaactat
    56101 cgtctgatat tgggtcatct gcgtctccga acagtcgaag cggcgtgcca ggtcagaacc
    56161 cgaccttgtc gccgcccttg agtttgagga tgtcacgggc ttcatcaggc gttgcgatct
    56221 ctagccctaa gccctcgatg atgtgacgga cacgggtcac ctgcgcggcg ttggaaggag
    56281 cgagcacgcc cttgtcgagc cagagattgt cctcaaggcc gacgcggaca tggccgccca
    56341 tggcggctgc ctgcgccgcc accggcatct ggctgcgccc cgcgccgagc acggaccatt
    56401 tgaaattgtc gccgaatagc cggtcggcgg tccgcttcat gtgcagcacc tcctccggat
    56461 gcgcgccgat cccgcccaac agaccgaagc aggtctggat gaagagcggc gcctgcacca
    56521 acccctcagt ccagaaataa tggaggttat ggaggtggct ggtgtcgtag cactcgaatt
    56581 cgtagcgcgc gccggtttcg ttgagggttt ccagcgcgta gcggatgtcc ttgaagctgt
    56641 tgcggaagac gagatcatga gagccttcca gcatcgccgg ctcccagtcg tgcttaaact
    56701 ccttgtagcg cttgagcatc gggaacaggc cgaagttcat cgagcccatg ttgaggctcg
    56761 cgacctctgg cttccacact gcagccgggc gcactcgctc ctcgactggc atatagggtg
    56821 agccgcccgt cgtcaggttc accacaacat tcgacgattg cttgatcacc ttgaggaacg
    56881 gctcgaacgc ctccggactc tggtcgggtc ggccatcctt gggattgcgg gcgtggaggt
    56941 ggacgatcgc tgccccagcc tgcgccgcct ccagggcgtt ctctgcaatc tccgccggcg
    57001 tcaccggcag atgcggcgac atcgacggcg tgtggatcga cccggtgatc gcgcagctga
    57061 tgataacttt gctcatggct tatttcgcgg ccatgcggat cgatccgtcg atccggacga
    57121 cctcgccgtt cagcatgtcg ttgtcgatca tgtggagcgc gagcttcgcg aactcctccg
    57181 gttcgcccag gcgtggaggg aagggaaccg agcggccgag cgattccagc gcctcgtcgg
    57241 gaaggatctt gagcatcggg gtcaggaaga tcccgggcgc gatcgtcaga actcggatcc
    57301 cgaaacgcgc aagctcgcgc gcggcgggaa gcgccaagcc caccacaccc gccttggacg
    57361 cagaataggc gggctggccg atctggccgt cgaatgccgc gaccgaggcg gtgttgatga
    57421 tgacaccttg ggtctgcttg ccgtcgcctg attcttggtc gcgcgagatg acatcggcga
    57481 ccagacgcat catgttgaaa gtgccgatga ggttgatgcg aacgaccttc tcgaacgccg
    57541 caagcgaaac ggggccatcc ttgccgagaa tcttcatcgg cgtgccgacg cctgcgcaat
    57601 tgacgagaat gcgcggtgtc ccaagttccg atgcgatcgt tgccaccgtc gtctcgccgg
    57661 cctcggcatc ggcgatgtcg attgcaaacg cccgtccgcc gatgcgctcg gctacggcgt
    57721 tcgctgcatc gccattgaga tcgagcacgg cgaccttggc accgccctca gcgagcttga
    57781 tggcggtcgc ctcgccgagc cccgatccgc cgccggtgac gatcgctacc ttgttcttga
    57841 ggtccatgga ttcgctcctt gattgttgtc ggccctcatg cgggcgcgta cagattgccg
    57901 agacgcatgc cgccatcgca gtcgatcgtg gttccggtca cgaagctcgc cgcctcgctg
    57961 cacagccacg ccaccgcatt acctatgtcg atgcgttgcc cgaagcgcgg cagcgccaag
    58021 gcgccgcgaa tgcgctctgc gagttcagcg tcggtcgcga gacgcgccag gccttcggta
    58081 tcggcgatgg gaccgggcga gacggtgttg accctgatgc cttccacgcc ccattcgagc
    58141 gccaggcagc gggtcagcat ctcgattcca gccttggccg agcaggcatg ggcttgcatc
    58201 ggcatggcgc gcgcggcttg gggcgccgtg atgttgacca gcaccgctcc cggtcgcttg
    58261 aggtgcggat aagcggccga gagcacattg aaggtgccga ggaggtctat gtcgatgatc
    58321 gtcttgaagg cgttgggcga catcttggcc gccggcacga agaaattgcc cgcggcgccc
    58381 gatagaacga tgtcgatcgg tcccttggtg tcggcgacct cggtcatgat cgcctgcata
    58441 gtagttgcct cgcgcacgtc gccggcatga tgggtgacgt cgagaccatc ggcgcgcagg
    58501 gtatcggccg ctgcggcgat tcgcgccggc gagcggctga cgatcgcgat ctgtgcgccg
    58561 agcgagccca gccggtgggc gacacccagg ttgatgccgc tcgatgcgcc tactacgaca
    58621 gcgttgcggc cggacaggca ttgggggtca agggacatgc atggatatcc tgattattag
    58681 cgggtgaaga cggccttggt gcgtgtgagc ggcgccgcga gggctgccct tacatcctcg
    58741 cggcccgcga gcgccacgat ttcgcgggtc tcctcgtcga gatgcgttgc aaggctgttt
    58801 gtcgcggcgg cgtccgtcag ccgccgaatg gccgcataag ccgcggtcgg tccttccgcg
    58861 agttccctcg caagatcaag cgcggccgcg tccaacttgt cgtcggaaac cttgcggtcg
    58921 gcgacaccaa gcacgacagc ttcgtcggcc gacatgaagc gtgcgtccat gaggagggtg
    58981 cgggcgcgac gcgcacccag cgcacgggtc agaaaatagc tcacgcccgc atcagtgctt
    59041 aggccaagtc tggagtagcc tgtcgagaaa cgggtatttt ccgagcagag gaggtaatcg
    59101 gaggagaggg cgaggccaaa gccgcctcct atgacatgac cgcgagccgc agcgacgatg
    59161 ggaacgccga tgccgatcag acggagctgg gctgcgtgaa agccgtgggc catccggtcg
    59221 agttcggcgg caacgtcgct ccgggcggcg agcagatgct tgagatcgcc gcccaaggaa
    59281 aaattcgcgc cctccgcggt gatgaggacc gatcgcggtt cgcatgcccg cacggcatcc
    59341 accgcggcgc ccaggtccgc gatcaatgca gcgttgatcg cattgccggc atcgggccgc
    59401 gccaagatga tccgggcaat gctgtccaag tgttcgaagc gaacgagagg catgctcagc
    59461 gtccggccga gagacagttt gtatcggctg cttgcggagt ccaggtcatt ccattcctct
    59521 ctatgccggg gttggcggcc tgctcttcat acgatccggg ttcttctgtc cccccaatag
    59581 cggtcgcgca gcaagcgctt gtagagcttg ccggtaggga ggcgagggag ttcagcctcg
    59641 aaatcgatcg agcgcggaca cttctggtgc gacagatgcg ccctgcagaa gttcaccagc
    59701 tcattcacca gatcagagtt ggcctcgatg ccgggcacga cttggaccac agcctttact
    59761 tcttcgccga gatcctcatt cgggacgcca aatactgccg catccgcgac cttggggtgt
    59821 gtgatgagga ggttctcgca ctcctgcgga tagatgttga cgccgccgca gacgatcatg
    59881 aaggtggcac gatcggtcag atagagaaat ccgtcgtcgt cgacgtagcc cacgtcgccg
    59941 accgtgctca tggagccgtc ctgcgatcgg gcctccttgg tctttgccgg gtcgttgaag
    60001 tattcgaaag gcgtcgcggt cttgaaccaa agcgtgcccg gcgtttcctt cgggcagggt
    60061 tgcatattct cgtccagcac gtgcagttcg ccgagcagaa cctttccgac gctgccgcga
    60121 tgcgccagcc attgctcgct gtcgcaggcc gcgaatccga ggccctcggt cgcgccgtaa
    60181 tactcgtgaa tgatcggtcc ccaccatgcg atcatctgct ccttcacctg gaccggacaa
    60241 ggcgcggcgg cgtggattat cacctcgagc gatgacagat cgtaactcga ccgcgtggcc
    60301 tcgggcagtt tcagcatgcg cgagaacatc gtcggcacga gctggctatg ggtcggccta
    60361 tacctctcga ccagttgcag atagtgctct ggatcgaacc tctccatgat gatggcagta
    60421 ccgccgttcc ggatcacaag gttgacggac gcctgcggtg ccgcatggta aagcggggcg
    60481 ggagacagat agacgagccc ttcgctatag cgccacagct tctgaagaaa atcaaagagt
    60541 ggtagttgct gtgacggagg ctggtcggga agcgggcgga gaatgccttt tggcctgccg
    60601 gttgtccccg acgaatacaa catcgcggtg ccaattgtct cgtcggcaat cggcgtggcg
    60661 ggaaagctgg cggtggcctc gtcgagattg agtatacgat cgccgtcgcc cggaccgttc
    60721 acgaccagaa cggcttcgac cttggggcac tggccgattg cggtgagggc ggtcgcgcgg
    60781 gtctcctcgg atacaatcag aatcccggat tcgctgttat tgacgatgta agcgagctcg
    60841 tctgcggtca gaaacgagtt gacgcaagtg aagtagagcc ccgcgcgctc gcccgcgccg
    60901 cagctttcga tatagcggtc gttgttctcc atgaagatgg catagtgatc gaggcgcttg
    60961 aacccgcggc tgcgaagaaa gtgcgcaagg cggttggtgc gttgttccag ctccgcatac
    61021 gttacggcta cgccggtttg cgccatgatg aacgccggct gatcggttcg aattttagca
    61081 tgcacacctg gatacattgg aagtcgctcc ctgatgatgg ttgtgccgaa ttatttcggt
    61141 ctgttatttg gtagagtacg ctgttttggt gtcggaccgt gggcaagaag ggcagcgcga
    61201 ttggcgttaa agaaagctgg agcgcaacgg cgcccgttga gtaggacaag atagttgcgg
    61261 ttcacgcgtt tgagatgccg ttgagcccgg atccggtcgg tcgattgtct cccttggcgc
    61321 gccgtcactg catcgctccc tcacccgaca cgttgctcgg cggtcagcgg caaacatgcc
    61381 agctcatgac gttgaagctt gccggttgtg ctgcggggca actcctcgga gcgcatgaac
    61441 tgcaggtagc gcgggcgctt atagccggcg agccgctcgc gacaccagtg gaggatttcg
    61501 gcgccgtcgg ccgactggcc ttggcggagg acgaccacgg cctttatcgt ctcgccccaa
    61561 tgcgggtccg gtacgccgaa cacacacgct tcttgaacgg ccggatgtcg gctgagaacg
    61621 gcctcgacct ctgcaggata gacattctcg cccccggtct tgatcaggta tttcttgcgg
    61681 tcgacgaaag tgagcgtgcc gtcctcctca cgccgcaaca ggtcgccggt atggagccaa
    61741 ccacccttga acacctcggc attggccgca tcgtcatcga gatagccgga catgatgctc
    61801 gggccccgca ccacgcattc gcccggctga ccaacaggca tctcgttcat gtcttcgtcg
    61861 acgacgcgga cctccatcaa cggagagggt cgtttgcgta gcgacggcac ttcgcctggg
    61921 ccggaacagc cgtgcgcgag cagatagctc gactcgctct gcccgaaggc gtcgtaaaaa
    61981 tcgatgccga aagtctccgt cagcttcgcg acgacgttcg gcatcatgtt ggcgtagccg
    62041 atggcgaagc gcaggctcga cagatcgtgg gcctgccgct gcgggttgtt catgaagtcg
    62101 gtgatcacgc cgggcaggag cagagtccag ctcagccgct cgcgctcgat gcgctcatac
    62161 atggccggga tatcgacgcg cttgaatgtt gcatagatgc cgccggtgac gaatgttgcg
    62221 tagagagatt cgtctccgcc gcagtggaag agcggcagcc agccgacgaa accgtccttc
    62281 tcagtcagtc cgaaccattg agcaagacgc aagccgcggg tggccgccgc tcgctggctc
    62341 acgagtgcgc ctttgggctg accggtagtg ccgctagtga aaaggatgtt atggatgtcc
    62401 gcgccctcgg cgatccgagg cggctcgccg gcgctgcttt cggcaagaat ggcatcgtaa
    62461 tcgtcgaagc cctgcgccgg ctcgaatgcg atccagtcgc cgacgtccgg aaactccgac
    62521 cggaggcctt cgagcaacgc cgccagcgtg ccggaggtca ggacggcact gggacgagcc
    62581 ttgcggaggc acgcggcgat ctcatcgggg cgaagccgaa tgttcagcgt cgcaaccgtg
    62641 atgccgagtg cggccatggc cgcatagcat tcaacatatt ccggacgtgt ctccgagagg
    62701 atcgcgatcg tgtcgccgcg gtcatatccc ttggcacgca gggcattggc gagccgccag
    62761 gctcgctcgc cgacctcgcg gtagcggcgc tgcccgcctt cccaggtgtc ggcggtgcgg
    62821 tcggcgaaga cgcggaatgc gcgtcggaag agagcgtcca gcgtgatgtc cgccgcgatg
    62881 gcggcatcga gttgccagtc ggctgcgccg gaagacgact tactggtaat aggccgcata
    62941 ggtgcgcctc agcttcgctt tttcgatctt gcccgtggcc gtcttgggca gctccgaaac
    63001 atggatgacg gccttcgggc atttgaacgg gcttagccgc tcccggacgg ccgaaagcag
    63061 catgttcgcg tcaatggatc cgccattctg ggtaacgacg acggcggtga tcgcttcgcc
    63121 ccagtgccgg tgcggcagcc cgattgcggc gacttcgagc acgtcgggat ctgcggcccg
    63181 gatcgcgagc tcgacctcga tcgatgagac gttctcgcca cccgacttaa tgacgtcctt
    63241 gaagcggtcc tcgaaccaga gaatcccgtc aacgtcgaaa tggccggcgt cgcccgagtg
    63301 gaaccagcca tggcggaaag cttctgcggt cgcctcggga ttgtgcagat aacccgccat
    63361 ggtctgcggg ctgcgataga cgatctcccc ggtgctgccg gtcggaagca gctcgccgtt
    63421 cgatgccatg atggcgacct gtacgttgac ggccggcgtg ccgactgcac cgggatggct
    63481 cagctggtgc tggggcttga agaatgtcga gaccgggctc atctccgttt ggccgaacat
    63541 gaggctgaag tcgcacccga agatctcgat cgcccggcga agctcggcct gtggcatcgg
    63601 cgccatggcg tagacagtca ggcgcagact gctgacgttg cggggatgtg cgagctgccg
    63661 ttcgacgagc tgctggatca tcatcggcag cgcgaagagg acgctgatgc gctccctttc
    63721 gatggcttcg tgaaggccaa cggcgtcgaa gccgcgctgg atcaccatcg aggcgccgac
    63781 tgcgattgcc ggtgtgcaga gcgcgttgag ttgggccgtg tggaaaagcg gaagcaccac
    63841 gctcagccga tcgttctcgg tcatgcgtgt atcgatcgcg gtcccgagcg attccaggta
    63901 gatcgcaaga tgggtgccga tcacgccctt cggcgccgag gtcgtgccgc tggtgtagag
    63961 ataggagagg ggagcccggt cctcgatctc gacatccgga atgtcggagg tctgatcggc
    64021 cgcgagcgcg tcaaagtcga acagcggcat gtcgccgata ccgtcgggag tagaagcgcc
    64081 tgacgcctgc cagtccatca cgacgatacc gagcagcggc cccggacctt cgacgacagc
    64141 gcagaattgc tgcagtaggc ctgtttcgac cacggcagcg cgagcgcgtg agtccgtcag
    64201 gacgtggctc agttcgtgct gtcgccagag caggttgatg gggacgcaga ccaggccaag
    64261 ccgcgcgcag gcgaaataga cggcgaggaa ttcggcgcgg ttgcccgaca tcagcgcaag
    64321 tgcgtcacct ttctgaaggc cgagcgcgga aagaccatgg gcgatccggt tggtccaggc
    64381 gttgaagtcg cgataagacc agcgcctgtc gccgtcgcag atcatctccc gctctggaaa
    64441 ccgctccgcg ctccgggtga cgaggtcacc aacattgaca cggtggatta ggtgactgct
    64501 cacgtccatt cgtcagctcc atggtttttg cgctgcggtg acaactacgg ctctacgggt
    64561 cctcgccgct gaaatagggt ggttttccgg tggtccatgt ctctccccaa agttggcccc
    64621 ctccgtctac cgtcaacgtt tcgccggtca cgaacccgcc ggaaggaccc gcgagataga
    64681 cgcaggcttc ggcgacgtcc catggcgaac ctgcgcgcat catcgggttc gatcgggggt
    64741 aggctgttcg cgcctcgggc gtatagacct tccagccttc ggtctcgatc gcgccgggag
    64801 ctacgcaatt gacccggatc ttcagggggg cccattcgac ggcaacgctg cgcgaaaggc
    64861 cgatgacgcc ggatctcgcg gcgatcgtgt gcgcgacgcc gtagaggccg tgcgtcgtga
    64921 caaccacgat gttgacgatg ctgccgggat atttgtggtc gcgccagttt tgtgcggcgg
    64981 cctgcatcat gtaccaggtc ccgttcagat tggtattgat gaccgcattc cagcccttta
    65041 cggaaaaatc gatggccggt tgaggaaact gcccgccggc gctgttgata aggacgtcga
    65101 cgcggccatg cgtcttccag gccgcatcga acaaagcttt gaccgcatcg ggatccctga
    65161 tatcggcccc gtatgcggat gccttcagct tccgtcctgt caggttcgat acgagagtat
    65221 cgagcctttc ctggttgcgg ccaaccaaga cgacatgcgc gccgagccgt gcgaacagcc
    65281 acgcggtagc gcgccctatg ccaccggcgc cgccggatag cactacaatc atgtctctaa
    65341 gggcatcgga tgcgaacgca gtgcgatgca ccgcaagctc atcgtccgtc agcccgagtc
    65401 catccggccg ttccggtgtc tgattgtcag ccatgggtgg ccatgtcctt gagcagctcg
    65461 cctgcgatta tcatctttcg gacttctgtc gttccggcgc cgatctccaa cagcttgatc
    65521 gaacgaaata gccggttgat ctccgactcc cagatgtagc cgctgccgcc gtggatctgc
    65581 actgccttgt cgaggactgc gtggcaggcc tctgcagcgt acatcacgga agccgccgtc
    65641 agcatgtgga tatcgccgcg cccgccaccg ccgacttcga gcggcgcggc ctccgccaac
    65701 gtgcgatagg tgaaggtccg catggtttcg acctgaacgt acatctccgc gagcatcgac
    65761 tgaaccattt ggaacgatcc gatgggcttg ccgaactgct ggcgggtctg cgcgtagtcg
    65821 atggagagct gcagcgcccg ttccgaaatg ccgagacaca gcggcgaaat catagcgcgt
    65881 tcgaggtcca gtccgctcat cacgatcgag acgccgcgat cttcgccgcc gacaaggttg
    65941 tccgccggca cgcggcagtc ttcgaacacc agttcggcgg tctggctgcc gcggtagccc
    66001 atcttggtga gcttctgggc aaccttgaag cccggaaaat ccttttcgac gatgaaagcg
    66061 gaaatgccct gcgcaccttt cgcgcgatcg gtcttggcat agaccaggat gacgtccgcg
    66121 acaggtccgt ttgtgatgta catcttgccg ccgttgagga cgtagtgatc gccttcgcgc
    66181 cgggcggtgg tgcgcatcga tcccagcgca tcggagccgg cgcccggttc ggtgaggccc
    66241 agcgcaccga tctttcggcc cgtgcagagg tccggcagat atttgcggcg ctgctgttcg
    66301 ttgccgttgc gaaagatgtt gttggcgcac aggttgtcgt gcgccaccca ggacagcgcc
    66361 atggcatgat tccagcgcgc gaagccctgc aacaccaggc cggccgctag caggtccatg
    66421 ccggcgccgc catactcttc cgggatggtc gcgccgaaga aaccgttatc cccgatcttg
    66481 ggaaaggctt cgtctggcca ccattcctcg cggtccatcc gttcggacag gggataaagt
    66541 tctttgcgcg cgaaacgatc ggcctgatcg aggatggtct gttgctcttc gtcgagagca
    66601 aacatcgacg cacgattgtc accgctcaag atggcgtggg acgtggtcat gcgccggtct
    66661 ccttcccatt ttgctgcgtg ccgcgttccg agacggccgt ggcgtataat gaattacatt
    66721 cattatacga agccgggcgg ttgtcaaccg tgctgacgtc gccaggagag gtaagggagg
    66781 ggaggcaggg gcgcacagcg gcgaccgttg tgtttgcgga ccggttcctg ataccggcaa
    66841 tgagttggaa atcggtcggg agatttatcc ggcggcgacg gcagattgcc gcttcattag
    66901 gatcttggca gtaccggtct gcacggtttc atcgcgctgg ttgacgatgt ccagtagcag
    66961 ttccacgaca ccgcgatccg gctttcgcgc cggcctcttg gcgaccacat ggatccgcgt
    67021 caagatcgtg tcgccgggcc gcaccggtcc ggtaaacgtc cacccgatgt tgagcagcgc
    67081 cagcgctgtg ccatccagta gtcctgcgcg ggatgcaagc ccgaaggcca tgcccacgag
    67141 catcggtccg tgggcaatac gcccgccgaa atcgctttgc gcggcgaaca cgtcatcggt
    67201 gtgcagcggg ttgaagtccg ctgtcagccc cgcgaactga acgatatccg cgtccagaat
    67261 ggtccgacgc ggcgtcgcaa agcaggcgcc gatttcgaac tcttcgtaat accgtcccat
    67321 cgttcaatca cctgctgtct ttgaactgcg cttcttgcgt tggcgcatgt cgaacacgcc
    67381 cggaatggtg cgcggatcca cgccatccgg aaaaatatga tgcttctgga tcttctgcgt
    67441 gccggtggtc ggcagaacgt cgacgaaatg gatccagccg ggggtcttga agtaggcgag
    67501 ttcggcgtaa cagtgctgga acagcacgtc cgcagcgacg tctgtgtcgt gtgcgtccgc
    67561 cagcaccacg cacgcgaaca cctcctcctc gcgcagttcg tcggggacag cgagaaccgc
    67621 gacctgcttc accatgtcgt gggattggag cacggcctcg atctcggcag cggcgatatt
    67681 ctcgccggaa cggcggatga tgttcttctt gcggtcgacg aaatgcagca ttccgtcgtc
    67741 gtcctgccgg acggtatcgc cggtatggaa ccagccgccg cgccacgctt gcgcagtcgt
    67801 ctggtcgtcc ttcagatagc cagagaagaa gccacggcgt ggcgttgcct ctgaatgacg
    67861 gatgaccatc tcgcctggcg cgccgaccat gacatcctgg tcttgctcgt cgacgacgcg
    67921 gacgtcgatg ccaggggtcg gtcgtccgaa cgcacgggta ccaaccttgc gcggcggctc
    67981 gtgatcgagc agcacgcgta ccatttcggt catgccccag acttcgatca gcggaaagcc
    68041 gaaccgctgt tcgaacacac cgtggagttg cggctcggcg cccgccccaa gaccaaagcg
    68101 gatgctgtgg ctgcgctctt cgtcgcattt cggcattccc agcagaagcg ggacgataac
    68161 gccgagatag tgcacgatgg tcgcgcccgt ctggttgacc tcgggccacc agcggttggg
    68221 atggaagcgg tcaggctgaa tctgacagct gccgttgagc atcgcgcagt ggaacgacac
    68281 gatcgaagaa ttgacgtgat acaccggcag gggattataa atgcgttcgc cctgcgcgcg
    68341 gaacgaggcg agctgtccgc gcgtcgcgta ccacgctccg gattcaagct cataaccatg
    68401 cgacagtatg cagccttttg gccggcccgt ggtccccgag gtatagagga cgctggcctc
    68461 gctcgcagcg gttacgggat cgttgcgtcg gggtcgcgcg ggtgccccaa gcgacctgag
    68521 gtcgtcgaac tgcgcgaccg gcgctttgtg cgagccggcg tccattcccg ctgtgagctg
    68581 cttgcgacgc tcgccggaca cgatcgcgag gtccgcttcg gaattttcca gcagatacgc
    68641 gatttcgttg gcgcggtaat cgggattgat cggcacgcag gaaatgccga gcgtattgag
    68701 cgcgagcttg tgcaggaagt gctccggtct gttgtccagc aacatggcca cgcgatggcc
    68761 gtggccgaaa ccgtgggagc ggtagatttc gcagagctgc cggacctcgc gcgcggccgt
    68821 cgcaaaatcg atctcgtagc cttgccggtg atagcttcgg tgcgcccctg ccggcgcggc
    68881 caggaacgcc cgtggagcat aagcctgcgt ggcgcgggcg aacgcatcgc cgattgtatt
    68941 cgtttcgtcg atcgggatca cgccgacgtt cctcctttgg tttattcgga ccgcatcgtg
    69001 agctgcgcga cctcgagcag gcgcctggcc tgcttcagat ggggcatatc cagcatctgg
    69061 ccatcgaggc cgacggctcc ggtcgacgaa ccttcgaacg ccgcgaccac acgttgcgcc
    69121 caggcgatct cctgatcgga gggggtgaac gcgctgttga tgatctcgac ctgatcagga
    69181 tggatggcca tcataccgcg aaagccgtct cgcctgctcg ctgcggcgta agcctgaagt
    69241 cccgcgcgat tgcggaagtc cgggaagaca gtctcgatgg cgacgacatc cgcggcggcc
    69301 gccccggcca ggcagagcga gcgcgacaac tgataggtaa acgtcagccg gccgtcctga
    69361 ttgcgattgg cgctggcgcc gagcgcggcg gcgaggtctt cggcgcccca tgtcagtgcg
    69421 tagagacgcg cactcgcgcc gccatatttg tcgagtgaaa acagcgcctg gggcgtctcc
    69481 gtcgccacgg gcaggatctt gacgtggccc gcgggcacgt cgaacgcccg ctccagcgca
    69541 tcgaggtaat tggacagcgt cgtcacgtcg cggcccgagg tcaccttggg cagcatgatg
    69601 ccatcgggcc ggctggcgat gatagccgca agatcggtga gcgcatgagg cgtgtcgagc
    69661 ggattgatcc ggacccacag ctcttggcgc gaccggggtg accgggcggt gaggaaatcg
    69721 cgcaccagca cgcgtgcccc cggcctgttc ggctcggcga cggaatcctc gagatcgagg
    69781 atcagcgcgt cccccggtcc cgcggcgccc ttttccatct tctttaggct gtcccccggc
    69841 gtgaacaaaa acgagcgcaa tgtcattgcg ggctccgctt gaccagcacc gaccgcagac
    69901 attgcgcgac gacttcgttt ctctggttca ggcagcgatg ttcgaacacg acgatgccct
    69961 gtccggggcg cgaacggctc tcgcgcttct cgaccacctt ggtctccgag cgcagcgtgt
    70021 cgccgatccg taccggtttg ggaaacacca ctttgtccat cccgaggttg gcgaccagcg
    70081 accccagcgt ggtgtccgag accgagacgc cgatcatcag cccgaaggtg aagatgctgt
    70141 tcaccaggat gtgcccgaac tctgactttg cggcgatatc ggcgtcgagg tgcagcggct
    70201 gcgtgttgtg ggtgagcgca gtgaacagaa gattgtccgt ctccgtcacg gtccgcgtca
    70261 cttggtgacg aatgatctcg ccgtccacga attgatcgaa gaatttgccc gccacaggat
    70321 ttgctcacca cggttggaga tggatcggca aaatctgaaa tgatgaagtg tcacgccgtt
    70381 gcggccgaca attcatattt cgtttccgcc catctgcttg ttgaatttaa ttcattttag
    70441 cggcatcata tccattattg tccgatgggc aagagagact ggtcatagaa accgacaagc
    70501 gaagtggggc gctgatgact tagaacacag ccgacaagtc ggttctgacc agcagggatg
    70561 gcgcggtcgc ggttattcgc ctgaaccggc cggaccggtt gaatgcggtc gacgaagcca
    70621 tgatcaccga actgcgcgca gtgcttgaag cggcgaatct cgatcccgat gttcgtgcgc
    70681 tgacgatgat cggatcggag cgttcgttca cgtccggtgc ggatccgacc ttgttccacc
    70741 gggatctgga cagggcgccc gagacggcgg cgcgtctggt cgacgccttt cacggcatgc
    70801 ttcgctccgt gcgggcgatg ccgaagccgg tcatcgcgag attgagcggg tcagtcgtcg
    70861 tcggcggcct cgggctggcg ctcgcatgcg acctgtgcgt gatggcggac aacgcccggc
    70921 tgatctcggc atattccaag atcggcacca atccggatgc gggaacgacg tggtcgctga
    70981 cgcaccatct ggggcgcagg cgttcgctgg aagtgatgtg gctcaacgat ccgatcggag
    71041 cgcaggaggc gcttagcctt ggattggtga acagggtcgt tccgcagtcg cggctggaga
    71101 gcgagacgct cgcggttgcc aagcggattg ctgacggacc gcccgggacg acgggacgaa
    71161 tcaagtctct ggtcgatcag gcctgcgtct ctgatctggc cgcacaactc gacagggaaa
    71221 aggcggaatt cgtcgccgcc gcgggaagcc gagagtttcg tgaaggcatc accgcttctt
    71281 tcgaaaaacg ccctgcgcaa ttctgactgt gttcgcgccg accgcggcaa cgcattgcct
    71341 tattgaagtg gcgctgttga catgccaaga tgaatgatat tcaatatggg gattcagccg
    71401 tatgatgcag gaccttggcg cgcgcccttt tggttgataa cgatgcggtt caagtctcga
    71461 tcgcccgact acccaggagc agcgacatgc cggccataag tcctgaacgt atgcaggatc
    71521 gctatgatgc gatcctcgac gccgccaaac gagccttcgc cgaaagaggc ttcgaaggcg
    71581 cctcgatcgc ggacatcgcg cgaatcgcgg cgatctcgga cggtctagtt tatcgctatt
    71641 ttcgcaacaa acgcgagctg ctgtacggcg tactgcagaa attctatgag cgaatcctcg
    71701 tcgatctcga acttcaggtt ttcaagcacg atggtttcac ggcgcggctc gaggccttga
    71761 tcaaacggca tatcgaggtg ttcgtgtcgg ataccgattt gtgccgtctc ttcatcgccg
    71821 aggtacgtac ggccagcgac tacgaaggct ccgcgattca ggagctgaac cggcgctaca
    71881 cttccgttct gatccggatc gtaaaggatg cggtcaaaac cggagaggtc agggtcgacg
    71941 tcaatccaag gctgctgcgc gatgtgctgt tcggcgcaat cgagcatctg gcgtggcgtc
    72001 acgtcaacgg cagaggccag cttagagccg catcgaccgc gcgcgaactg accagcatgc
    72061 tgacctcagg catttgcatc gactagttaa ggatatccgt ggccgattca tcattcccct
    72121 tcgactgcgt cctgattgcc aaccgcggcg agatcgcggt ccggatcatc gggacgttgc
    72181 gcaagctcgg gctgcgcagc gtcgtggtct atcacgaggt cgatgcgcgg gcgcccgcgg
    72241 tggcgttggc agaccaggcg atcgcgatct cgggccgcac accggtcgcg gcgtatctgg
    72301 acggcgagca gatcattgcc gcggcgcgat ccgccggcgc cggcgcgctg catccggggt
    72361 acgggttcct gtcggaaaac gccgccttcg cgcgttcggt cgaagccgcg ggcatcgcct
    72421 tcatcggccc gtcggccgat gcgatcgagc tgatgggcga caagatccgc gcccgcaatt
    72481 tcgtcgcgcg caacgaattt ccggtcgctc cgtcggcgat cgaggaagaa gatcctgcca
    72541 ccttcgtgga gcgcgcgcgg gccattggtg cgccgcttct ggtaaagcca tccgccggcg
    72601 gcggtggaaa aggcatgcgc atcgtccgcg acctttccgg actggacgat gcggttgcgc
    72661 aggcacgcag tgaaggtcag cgctatttcg gcgacggccg gctttatatc gagcgatttg
    72721 tggagcagcc gcggcatatc gaagtgcagg tgctcggcga tgcgttcggc aacgtcgtcc
    72781 atctgttcga acgcgaatgc tcggtgcagc gtcggttcca gaagatcatc gaggagaccc
    72841 catcgccggc tttgtcggcg cagacccgtc agaaaatctg cgagacggcg gcgggcatcg
    72901 cccgcgcggc cggataccgt aacgccggca cggtggaatt catctacggc cgcggcgaat
    72961 tctactttct ggaaatgaat acgcgcctgc aagtcgagca tccagtcacc gagatgatca
    73021 cgggcctcga tctcgtcgcc gagcagattg cggtggcggc ggggcggcgg ctccctttcg
    73081 cgcaaggcga cgtgcgcgcg catggccacg ccatcgaagc ccggctctat gccgaggccc
    73141 ccgagcgaaa cttctcgccg acgaccggga aagttctggc gcttcgttac ccgcaaggcg
    73201 agggcgtgcg cgtcgacagc ggcatcgccc agggccagac cattaccact gcgttcgacc
    73261 cgatgctcgc caagatcgtg gtccacgcca ccacccgcga cgaggccatc gcgcgggcgc
    73321 gaagggcgat cgaggagttt gtgcttctgg gttgcgagac caacgctgcg ttcttggcgc
    73381 gcatcctcgg cgaccaaggt ttcaaggccg gagaggtcca tactggctac ctcgacgaga
    73441 atcccggcat cgcccgggga ggcagccgag acacagatgc cgagacccgg ctgctggcac
    73501 tagccgcgct gctgacgcgc ccggtgcgcg atgcggcgga cgccgtgccg cctctccacg
    73561 cctcgcttgg aagctggaga aactgaggtg catcacagat tcgatctcga tggtgaaatc
    73621 aacgagctct ggctgtcccg cgggccggcg ggttatcacc tgcagatcgc ggacggctgg
    73681 agtgggtcgg tggctctcat tgaggagcgc gcgggtcatg gccgcctggt cgtcgacggc
    73741 aagagcgagc aggtcgccta cgtggtcgat ggtgacgtgg ttcacattca ttgccgcggc
    73801 cgcgcttacg cggtgcgcta cgtcgatccg ctgctggcac tggccagcgc cagtgacgaa
    73861 gctggccata acgtcgcccg cgcgccgatg cccggcgtcg tggtgtcggt gaaggtcacc
    73921 ctgggcgaca cggttgccgc cggcgccgtg ctgatggtga tcgagagtat gaaactcgaa
    73981 accccgatcc gcgcgccgca ggacggcgtc atcgaacgca ttcatgtcag ggacggcgag
    74041 agctttgatc gcgatgctgt cctggcgaca ctctcccagg ggaacggctg acatgcagcg
    74101 cctgcgctct ctgacggata cgcgcgccga cgatttccgc agcaatgacg ggcataaccg
    74161 caggctcgcc gccgaactca aggaacggca gcggatcgcc cgtttcgtcc gacccgagcg
    74221 ggacatcgag cgcttgggcc gccagaataa aatgttcgtg cgcgaccgaa tcgaggcgct
    74281 gctcgatccc gacacgccgt tcttagaact gtccacgttg gccggaaaca tggcctatga
    74341 tggcgaggtg cccggcgcgg cccaggtcat tggcatcggc atcgtgtcgg gcaaggaagt
    74401 gatcatccac gccgacgacg ccagcgtcaa aggcggcgcc tggtatccgc tgtcggtcaa
    74461 gaagatcgtg cgagcgctcg atatcgcgat cgagaaccgc cttccggtcg tgcatttgtg
    74521 cgacagcgcg ggagggttcc tgccgctgca agcgcagttc ttcgccgatc gctattacgc
    74581 cgggcgcatc ttccgcaacc agtccgttct ctccaagatg ggcgtgccgc aagttgctgt
    74641 ggtgatgggg cattgcacag cgggcggtgc ctatattccc gcgctcagcg actacaacgt
    74701 catcgtccgc ggcaccggcg cgatcttcct tggcggcccg ccgctggtga aggcggcgac
    74761 cggcgaggaa gtctccgccg aggagctcgg cggcgcagac atgcacacca gcgtgtcggg
    74821 gaccggcgac tatcccgcgg cctccgaatt gcacgcgatc gcgatcgccc gcgatatcgt
    74881 aggacgcttc gcgcatcccg agaagtcgaa gatcgactgg gttgaccccg agccgccggc
    74941 ctatgatccg gacgagcttt acgggattat cccgaaagac ccccgcgtgc agttcgacat
    75001 gcgcgagatc atcgcacgtg ttgtcgacgg cagccgcttt catgaatatc agcctcgcta
    75061 cggtcagtcg ttggtatgcg gcttcgcgcg cgtgttcggt tatcaggtcg gcatcctcgc
    75121 caacaacggc gtgctgttca gcgacagcgc actcaaaggg gcgcacttca tccagctctg
    75181 tgacaagaac cggacgccgc tgctgtttct gcaaaacatt acgggcttca tggtcgggcg
    75241 cgagtacgag cggcgcggca tcaccaagga tggcgccaag ctgatcatgg cggtggccgg
    75301 cgcgtcggtg ccgaagttca ccgtggtctg caacggctcc tatggcgccg gcacctacgg
    75361 catggccggg cgcgcattcg atccgcgctt catgttcacc tggccgcaat cccaggtatc
    75421 ggtgatgggc gcggaacagg ccgccggcgt gctgaccgac atcaagctgc ggcagcttgc
    75481 gcgaaacggg caggagctat cagagcagga gcatgctgcg atccgcgatc ctattctgga
    75541 ggactaccgc ttgcagtcga gcgcctacta cgccacctcg gaaatctggg acgacggcat
    75601 tctcgatccg gtcgacaccc gcaacgctct cggcatcgct ctcagcgcgt cgctcaatgc
    75661 gccgattgcg ccgccgcact acggcgtgtt cagaatgtag gagagggaca aggcaaagag
    75721 gggtaactga ccttcggacg tgagcactga acacgatctc gtagaaagcc gatatttcac
    75781 ttctccgggg gcgcgttaag cggttcgtcg gtcgatgatc gcaccgcttt gcactatcga
    75841 tccgttggga acaacgcgga ccatcccgcg cagcttcgtg acatcagtca gggtttgcga
    75901 tagcgctgtc gtgttaactt gaccatcact cgtttcgact tcgaaggtct cgaaggcggg
    75961 cgagtggtga tgaccggaac cgcggcggtg acgaaggcca atccggatat tcaggcggcg
    76021 tatctcggtg gcagacatgg gaccgactat ttggccgtga agcactaccg gcagcgcaga
    76081 cgctggcttt cctgacaaaa cggccgatcg actggaaggg gccatgccgg gacgacaaag
    76141 aggtgccgtt gatcaaacaa agggagtcga aatgggcatg ccgaacaaga tgatcgtgac
    76201 atgcgcggtg actggctcga ttcacacacc gagcatgtcg ccctatctgc ccgtgaccgc
    76261 cgacgaaatc gccgaagccg cgatcggggc ggcggaggcg ggcgcgtcca tccttcacct
    76321 gcatgcgcgg catccgcaga caggcaagcc ggatcagacc ccgcaagcgt tcgaaccctt
    76381 cctgaagatc catcaagcag cggtcgaact gtatcgtcaa cctgacgacg ggcggaagcc
    76441 cgttcatgtc cgtggaagaa cgattgaggc ctgcggcgac gttccagccg gaggttgcgt
    76501 cgctcaacat ggcatcgatg aatttcggtc tgttcccaat gctccagcgc tatagcgagt
    76561 tcaagcacgc ctgggagccg gagatgctgg aagcgtctcg ggatctggtg ttccgcaact
    76621 cattcaagga tatcgaatac gttctgtcca ccatgtcgca atccggaact cgcttcgagt
    76681 tcgaatgcta cgatacggcg catctttata accttcacca tttcatcgag ctcggcctgg
    76741 tcaagccgcc gctcttcgtc catgttcggg attctaggtg gcatcg
//
LOCUS       DATGEM010000072        10309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_549880, whole genome shotgun sequence.
ACCESSION   DATGEM010000072 DATGEM010000000
VERSION     DATGEM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549880"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1657)
                     /locus_tag="VKQ27_02400"
     CDS             complement(86..1657)
                     /locus_tag="VKQ27_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY87813.1"
                     /translation="MSDFQDIAWRPRGSVWLVAGAATLAPFMEILDTTIVNVSLPYIA
                     GTLSASNDDATWTLTSYLVANGIVVPVAGWLGKLIGRKRYFLTCIAMFTVCSFLCGIA
                     TSLPQLVVFRLLQGLFGGGLQPSQQSVILDTFEPSQRGKAFSLVAIAVIFAPIIGPTL
                     GGWITDNYSWRWVFLINVPVGIVAFISVMQLVEDPPWVQRDRAHLRDIDYGGLGLIAL
                     GLGSLQIMLDRGEDLDWFGSPTIRLFGLLAAVGIIGGITWLLMVEKPVVDLRCLKDRN
                     FGVGVLMVSGIGAILYSSNVLIPVMAQQWFGYTALWAGLLLSPGAAVMILFIPVVARV
                     VLPNVQTRYVIGFGFFVLGCSAAFACRLTPQLDFWTMALFRAFQTVGLAFLFVPNSTL
                     SYASLPRSLNADAAALYSMFRNIAGSVGIAVITALGAQRSQVHRAYLTDHLTPYDQGY
                     QTLLSHDTRALTAMGHTAAGAHDMAMGLINHTLDQQAAILSYVDLYAYTAIAAFCLVP
                     MTLLFRAGVAGRPGR"
     gene            complement(1663..2814)
                     /locus_tag="VKQ27_02405"
     CDS             complement(1663..2814)
                     /locus_tag="VKQ27_02405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLY87814.1"
                     /translation="MDDNTPTNLPPPVATTRAGVRLDQDLLSTRRRDRRAARAKWALL
                     LIVLAAVAAAVAYWYATKDAVTTDDAYTDGHAITVAPQVSGTVVSLDVTDNERVKAGQ
                     TLLRIDPRAYVAARDQARGSLQVAEAQLADARIALESARIDYPAKLSAAQAQLAAAKA
                     TQFKATADAKRQRSLPKQATMQQDVDNAEAALRSADAQVDQAQAAVRQAELVPQFVGE
                     AEAKVHQLEAQVALAHAQLDQAELNLSWTNVTAPQDGWVTKRNVEQGNYVQPGQAVLS
                     LVTPDIWVTANFKESQLERMRVGQKVDIGVDAYPQLHLKGHVDSVQLGSGSRFTAFPP
                     ENATGNFVKIVQRMPVKIIIDSGMDPNLPLPLGLSVVPTVRLDDTTAAK"
     gene            complement(3323..4933)
                     /locus_tag="VKQ27_02410"
     CDS             complement(3323..4933)
                     /locus_tag="VKQ27_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87815.1"
                     /translation="MSGETDLIIGHWSKLPFAELEPFLASLRHSGFAGEVCIFVSDVA
                     PETVWQLQDHGVRVERPGQSAQPRMTQLSSRFFTSLDFLARHGGNYRRVMLTDLRDVV
                     FQSDPFAAAWPADIVFAQERCLLVDCPVNRAWVVQGYGNAVAENMRNCVISCAGTTFG
                     TVSGMLRYLAAITSELTSRPVAIEGGIDQGVHNYIVHMRPPAGAWLDTSDAIAATMHY
                     VPTDAVTMTPRGALIDGKLVPVLHQWQKHAAIAGYVRSDARFRLSPPPASAAVADGAG
                     TVVAYYHRERDAGWLEVFLCSLRSTGFMGALHCIGEFNADEIALLARHGGVPQQVRPG
                     DASMQIENVAHLLISQVLDRIAGDDVAEQVLVLDTVRAGFLRDPFQNSTIGLSVFCEG
                     PMRIGESEYNLHRLAMFMEGPERFAQQPIVSSMLFRGRMDHVRALYRKLFIEFVGRAE
                     LLRIAKSIQGAVNKLCHEGAFEFPIVVHPNAAEMLFDFWPSGMPVVTQPDIRVGGAVP
                     AIVLGGSLASEVMGALRRGLGLEKAGVC"
     gene            complement(4952..6580)
                     /locus_tag="VKQ27_02415"
     CDS             complement(4952..6580)
                     /locus_tag="VKQ27_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87816.1"
                     /translation="MANLILGHCARVQPRHLEPFLASLRRTTFAGDVCLFVEDVVADT
                     VAHLRAHGVIVCRAASSSPPAMAASASRYFNFLDYLARHDGAYANVLLVDPADTIFQS
                     DPFAAPRAADIVFTRIRRRIGNSPADHSAVATGYGETMALNICDCWASNPSVTLASAS
                     GMLRYLVAMTTQISSRDAVIAGAIDRGIHNYLVRMCPLAGAWVDADDQFAVAIDGVAG
                     DALEVAGQGILIDGRLVPIVTAWQDNTAICAHVAGAPRFHLSADMRGPFPAALLPASV
                     SRSGAARNAIVAFFQKGRDDGWLSLFINSLRCVAPRTSVHCIGDFSQQEQDRLSLAGY
                     TIHLAPAVALELCDNVAHVHLNEALERLAADPAGPPDHVLVLDSMRAAFPRDPFASAT
                     IGLSVFCETRERIADNEYNRVRLAFYVPESDSRLPLPVVSSSALRGSLPVVRAFYQNL
                     FAELLTRQDILSVTRVVQGAINKLCHLQQFGFPVIVHPNGAEVYFDFWESGLSIDTRH
                     GLRVGGTVPGVVLGGHPESTLMVWLRTDLGLQQG"
     gene            complement(6622..7842)
                     /locus_tag="VKQ27_02420"
     CDS             complement(6622..7842)
                     /locus_tag="VKQ27_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HLY87817.1"
                     /translation="MSDAPAMILVPPAFLRESALAAVLAALPEARVVGGAVRDTLANR
                     AVTEVDLATPQTPEAVTKALQAARLRAVPTGIEHGTVTAVSDHQGFEITTLRRDVETD
                     GRHAVVAFTDDWRADAARRDFTINAMSMSRDGAVYDYFGGIADLRAGLIRFVGDPATR
                     IAEDFLRILRFFRFFARYGAGTPDASAAAAIRHGLHGLTGLSVERVWSELSRILAAPD
                     PRGAVLLMDELGVLGAVLPEGAEPSGLGRLVAAGAPAEPVLRLAALLTGDAAVVAARL
                     RLSAADRDRLVALRSGPVPQPGDDDATLRRLLSDAEPALLIDRAWLAGGGASEWAALR
                     QRLAAQTPPVFPLEGRDVLALGEPEGPRVGALLRAVRRWWLDGGCVAGRDACMAELLR
                     QWRGEGERTLSRDG"
     gene            complement(7839..8084)
                     /locus_tag="VKQ27_02425"
     CDS             complement(7839..8084)
                     /locus_tag="VKQ27_02425"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HLY87818.1"
                     /translation="MLRLVELALFLSPFIVFAAWRFFAIESGPSIRFVVVAACLLAVL
                     AAALVWLGEDRAIPGDSAYAPARFENGHIVAGHGVPQ"
     gene            complement(8068..8685)
                     /locus_tag="VKQ27_02430"
     CDS             complement(8068..8685)
                     /locus_tag="VKQ27_02430"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLY87819.1"
                     /translation="MTGEELRARLNEIVAAPLSGEITYGAEALDGLDRPPVPAAVLVP
                     IILGPAPGILLTKRSAHLAKHGGQVSFPGGRIDATDADAEAAALREAEEEIALDPLLV
                     KVLGRMDDHVTGTGYRITPVLGVLQPGFDLRPAPNEVEAVFELSIDVLLDPEAPKQHR
                     REVRGMWRDFWVWPHPDHYIWGATAAILVHLAQKLRAARPHAATG"
     gene            complement(8710..9327)
                     /locus_tag="VKQ27_02435"
     CDS             complement(8710..9327)
                     /locus_tag="VKQ27_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HLY87820.1"
                     /translation="MNEASSPAEFAMVVDQTPPPAVAFAETFGAGPCRQKRPRRPPVD
                     CGDLPFLIRRDGSWLYRGSPIARKELVCLFASVLKREDNGTYWLETPAERGRIEVEDV
                     PFVAVELDWSGDGRHQALSFRTNVDEVITAGPEHPLRVAHDILTCEPTPYLLVRPGIE
                     ARINRAVYYELVALGVPEWVGCRRMLGVWSCGKFFSLGELPPGEE"
     gene            9457..>10309
                     /locus_tag="VKQ27_02440"
     CDS             9457..>10309
                     /locus_tag="VKQ27_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY87821.1"
                     /translation="MATAQQGGLDASAILSEVEALGARIGKVRTAIGRVIFGQDEVVD
                     QTLITLLSGGHALLVGVPGLGKSRLVETLGVVLGLATKRVQFTPDLMPADIIGSEVLD
                     EAEGRRSFRFVPGPVFCQLLMADEINRASPRTQSALLQAMQEQRVAVSGVEHGLPRPF
                     HVLATQNPIEQEGTYPLPEAQLDRFLLEVDVGYPALDAERTMLLATTGAPEAPAEQAM
                     TADELRAAQALIRRVPVGESVVDAILVLVRSGRPETTEDDMVRRHVAWGPGPRAAQAL
                     MLASRARA"
BASE COUNT         2015 a         3537 c         3086 g         1671 t
ORIGIN      
        1 catcgtctgg cgcttgatcc gaattctgcc ggtctcgcgc cctaccgctt cgaggcgcgc
       61 cgcagctgac cgtgtcgccc gccggtcagc ggcccggccg gccagcgacc cccgcacgga
      121 acaacaatgt catcggcacg agacagaacg ccgcgatcgc cgtgtaggca tacaggtcca
      181 catacgacaa aatggccgcc tgctgatcca gcgtgtggtt gatcagcccc atcgccatgt
      241 cgtgcgcccc ggctgctgtg tgccccatcg ccgtcagcgc ccgcgtatca tgcgacaaaa
      301 gcgtctggta gccctggtcg tacggcgtca gatgatccgt cagataggcg cggtgcacct
      361 gtgaccgctg cgctcctaat gcagtgataa ccgcaatccc gaccgaacca gcgatgttgc
      421 ggaacatcga atacagcgct gccgcgtcgg cattgagcga tcgcggcaat gacgcatagg
      481 acagcgtgct gttcggcacg aacagaaatg ccagccccac tgtctggaat gcacggaaca
      541 gcgccatcgt ccagaaatcc agctgcggtg tcagtcggca ggcgaacgca gccgagcacc
      601 cgagcacgaa aaacccgaac ccgatcacgt agcgcgtctg cacattcggc aataccacgc
      661 gcgccacaac cgggatgaac aggatcatca ccgctgcgcc cggcgacagc agcagccccg
      721 cccacagcgc cgtgtagccg aaccattgct gggccatgac agggattagt acgttactgg
      781 aataaagtat cgcgccgatg cccgaaacca tcaggacgcc cacgccgaaa ttccggtcct
      841 tcaggcagcg cagatcgacc accggctttt ccaccatcag cagccaggta atgccgccaa
      901 tgatcccgac agccgccagc agcccgaaca acctgatcgt cggcgaaccg aaccagtcca
      961 gatcctcgcc gcgatccagc atgatctgca gcgagccgag ccccagtgcg atcagcccca
     1021 gcccgccata atcgatgtcc cgcagatgcg cgcgatcacg ctgcacccag ggcgggtcct
     1081 cgacgagctg catcaccgag atgaacgcga cgatgcccac cggcacattg atcaggaaca
     1141 cccatcgcca agagtagttg tcggtgatcc atccccccaa agtcggcccg atgatcggcg
     1201 cgaagatcac cgcgatcgcc accagcgaga acgccttgcc acgctgcgat ggctcgaacg
     1261 tatcgaggat caccgactgc tggctcggtt gcagcccgcc accgaacagt ccttgcagca
     1321 gccggaacac cacaagctgc ggcaggctcg tcgctatgcc gcacaggaac gagcacacgg
     1381 tgaacatagc gatgcacgtg aggaaatagc gcttccgccc gatcagcttg cccagccatc
     1441 cggcaaccgg caccacgatg ccgttcgcga ccaggtacga ggtcagcgtc catgtcgcat
     1501 cgtcattgct cgccgacagc gtgccagcga tataaggcag gctgacattg acgatggtgg
     1561 tgtccaggat ctccatgaag ggcgccagtg tcgccgcgcc tgccaccagc cagacgctgc
     1621 cgcgcggccg ccaggcaatg tcctggaaat cgctcacacc actcactttg cggcggtcgt
     1681 gtcgtccaga cgtaccgtcg gcacgacgga aaggccaagc ggcagcggca ggttcggatc
     1741 catcccgctg tcgatgataa tcttcaccgg catacgctgc acgatcttca cgaagtttcc
     1801 tgtggcattc tccggcggga acgcggtgaa gcgcgaaccg gaaccaagct ggacgctgtc
     1861 cacgtgcccc ttgaggtgca actgcggata agcatccacc ccgatatcga ctttctgccc
     1921 cacccgcatc cgttcgagct gcgattcctt gaaattggcg gttacccaga tatcgggcgt
     1981 gaccagcgat agcaccgcct ggccgggctg cacgtaatta ccctgctcta cgttgcgctt
     2041 ggtcacccag ccatcctgtg gcgccgtgac gtttgtccat gacaggttca gctcagcctg
     2101 atcgagctgc gcgtgcgcga gtgccacctg tgcctcgagc tgatgaacct tggcttcagc
     2161 ctcgccaacg aattgcggca ccagctcggc ctgccgcacg gctgcctgcg cttgatccac
     2221 ctgcgcatcg gcggatcgca aggcggcttc ggcgttatcc acgtcctgct gcatcgtcgc
     2281 ctgcttcggc aggctgcgct gccgcttcgc atctgcggtg gctttgaact gtgtcgcctt
     2341 tgccgcggca agctgcgcct gcgcggccga cagcttcgcc gggtaatcga tgcgcgcgga
     2401 ttcaagcgcg atacgcgcat ccgcaagttg cgcttcagcc acctgaagac taccgcgcgc
     2461 ctgatcacgc gccgcgacat aggcgcgtgg gtcaatccgc agcagcgtct ggccggcctt
     2521 cacccgctcg ttgtcggtga catccaagct gacgacggtg ccggacacct gtggcgccac
     2581 ggtaatcgca tggccgtcgg tgtaggcgtc gtccgtggtg accgcatcct tggtcgcata
     2641 ccaataggca acagccgctg cgactgccgc cagtacgatc agcagcagcg cccacttggc
     2701 gcgcgcggca cggcggtccc gccgccgcgt ggacagcagg tcctgatcaa gccgcactcc
     2761 tgcgcgggtc gttgctaccg gcggcggcag atttgtcggc gtgttgtcgt ccatcccccg
     2821 tcgcttggct gcgtatgccg catgtcccta atcgcaccct ggcagagatg ttgcaaatca
     2881 aagcgtcagg cagtcctgtt ttatcagttc tgccttttca gcagggtccg atcacgtatc
     2941 aatctagcat agttccagag cttctcaatc aatatagcaa cagtttcctg ctccattgca
     3001 ggcgctgggg gcatcattgc catcagttcg gcgttacgcg atgcggacaa cacacgggcg
     3061 acaatgatcc atacgcttcg ctgccgcacc ggcgcgagcc aacttcgggt agctgcccct
     3121 gctcttcgta tcgagtaaac caagcccctc atcaagtaag ggatttccca ggttacggca
     3181 cgataccgtt ggcattggca gcgagccgtc cgccacccac gatcgtctat tttcccgtta
     3241 tcatgacatc aatttttggt taatctgcag ccgtgcaagc aatcgcaccc cggaaaattg
     3301 caggcttggc ccggacacac catcagcaaa ccccggcctt ctccagaccg agcccgcgcc
     3361 gcaacgctcc catcacctcg gaggccagcg agccaccaag cacgattgca gggaccgccc
     3421 cgccgacacg gatgtccggc tgggtcacca ccggcatgcc acttggccag aaatcgaaca
     3481 gcatttcagc cgcgttggga tgcaccacga tcggaaactc gaaggcgccc tcgtggcaca
     3541 acttgttgac tgccccctgg atcgacttcg cgatccgcaa caattcggcc cggccgacaa
     3601 actcgatgaa gagcttgcgg tacagggcgc gcacatggtc catccggcca cggaacagca
     3661 tcgatgatac aatgggctgc tgcgcaaacc gctccggccc ctccatgaac atcgcgagcc
     3721 ggtgcagatt gtattcggat tcaccgatcc gcatcggccc ctcgcagaat accgagagac
     3781 caatggtgct gttctggaac ggatcgcgca ggaagcctgc gcgcacagtg tcgagcacca
     3841 gaacctgttc cgcgacatca tcgccggcga tccggtccag cacctggctg atcagcaggt
     3901 gggcgacatt ctcgatctgc atcgaggcat cgccgggccg aacctgctgc ggcaccccac
     3961 cgtgccgcgc cagcagcgcg atttcgtcgg cattgaactc cccgatgcag tgcagtgcgc
     4021 ccatgaagcc ggtgctccgc aggctgcaca ggaaaacctc cagccacccg gcatcacgct
     4081 cgcgatgata ataggcaacg accgttccgg cgccatccgc cacagccgcg ctcgccggtg
     4141 gcggcgacag acgaaaccgc gcatcggaac ggacatagcc ggcgatcgcc gcatgcttct
     4201 gccactgatg caggaccggg acgagcttgc cgtcgatcag cgcgccgcgc ggagtcatgg
     4261 tcacggcatc cgttgggaca tagtgcatcg tcgcagcgat cgcatcgctg gtgtcgagcc
     4321 aggcgcccgc cggcggacgc atgtggacga tgtagttgtg gacaccctga tcaatgccgc
     4381 cctcgatcgc caccggccgg ctggtcagtt cgctggtgat tgcggccagg tatcggagca
     4441 ttccactcac ggtaccgaac gtcgtgcccg cgcatgagat cacgcagttc cgcatgttct
     4501 ccgcgactgc attgccatag ccctgcacga cccaggcacg attgaccggg cagtcaacca
     4561 gcaggcaacg ctcctgcgcg aacacgatgt cggcaggcca ggcagcggcg aatggatcgg
     4621 actggaacac cacgtcgcgt agatcggtca gcatcacgcg ccgatagttc ccgccgtgac
     4681 gcgcgagaaa atcgagagag gtgaagaacc ggctcgacaa ttgcgtcatg cgaggctgcg
     4741 ccgactggcc tggccgctcg acacgcacgc catgatcctg aagctgccac acggtctcgg
     4801 gcgcaacgtc gctgacgaag atgcacacct cacccgcgaa tcctgaatga cggagcgaag
     4861 ccaggaacgg ttcaagttcg gcgaacggca gcttcgacca gtgccctatg atcaggtccg
     4921 tctcaccgct catcgtggca catctcttcc atcagccctg ttgcaaaccc aggtcggtcc
     4981 gcagccagac catcagcgta ctctccggat gcccacccag aacgacgcct ggaacggttc
     5041 ctcccactct caatccatgt cgcgtgtcga tggaaagccc cgattcccag aagtcgaaat
     5101 acacctccgc gccattcgga tgcacgatca ccggaaatcc gaactgctgc agatggcaga
     5161 gcttgttgat cgcgccctgc accaccctgg taacggacag gatgtcctgc cgggtcagca
     5221 actcggcgaa cagattttgg tagaaggcgc gcaccaccgg caggctgcca cgcagcgccg
     5281 atgacgacac cacaggcaat ggcagccggc tgtcactctc cggcacataa aaggccagac
     5341 ggacacggtt gtactcatta tcggcgatgc gctcgcgcgt ttcacaaaac accgacagac
     5401 caatcgtcgc gctggcgaac ggatcgcgtg ggaaggctgc gcgcatcgaa tcaagtacaa
     5461 gcacatggtc cggcggcccc gcgggatccg ccgccagcct ttccagtgcc tcgttaagat
     5521 gcacatgcgc gacgttgtcg cacagttcca gtgcgacggc gggagccagg tggatcgtgt
     5581 aacctgcgag cgaaagccga tcctgctcct gctggctgaa gtcaccgatg caatgcacgc
     5641 tggtccgggg cgcgacgcaa cgcaggctgt tgatgaacaa ggacagccat ccgtcgtccc
     5701 gccccttctg gaaaaaagcg acaatcgcat tgcgcgcagc gcctgagcgg gaaaccgacg
     5761 caggcaacag agccgccgga aacggaccac gcatgtcggc gctgaggtga aaccgcggcg
     5821 cgccggcgac gtgcgcacag atcgccgtgt tgtcctgcca cgccgtcaca attggcacca
     5881 ggcgtccatc gatcagaatg ccctgccctg ccacctcgag cgcatcaccc gcgacgccgt
     5941 cgatggcgac ggcgaactga tcgtccgcgt cgacccaggc accggcgagc ggacacatgc
     6001 gaaccaggta gttgtgtatg ccgcgatcga tcgcgccggc gatcactgcg tcgcggctgc
     6061 tgatctgcgt ggtcatcgcc accagatagc gaagcatgcc ggacgccgag gccagcgtca
     6121 ccgacgggtt cgaagcccag cagtcgcaga tgttgagtgc catcgtctcg ccatagccgg
     6181 tggcaacggc gctgtggtcc gctggcgagt tgccgatacg gcgccggatg cgggtgaaaa
     6241 cgatgtccgc cgcccgcggc gccgcgaacg gatcggactg gaagatggta tcggccggat
     6301 ccaccagcag gacgttcgcg taagccccgt cgtgcctcgc cagatagtcg aggaaattga
     6361 aataccggct cgcactggct gccatcgccg gcggacttga tgacgcggcc cggcatacga
     6421 tgacgccatg agcacgaaga tgcgcgaccg tatcggcaac cacatcctcg acgaaaagac
     6481 agacgtcacc ggcgaacgtc gtgcggcgca gcgaggcaag gaacggctcc agatgccgag
     6541 gctgcacacg cgcgcagtga ccgaggatga gattcgccac atcggtgccc gggaaggatc
     6601 gatgcgcagc ttagagtacg atcagccgtc gcgggagagt gtccgttcgc cttcaccacg
     6661 ccactgcctc agcagttccg ccatacaggc atcccggccg gctacgcatc cgccatccag
     6721 ccaccaacgc cgaaccgctc gcaataatgc gccgacccgc ggcccctccg gctctcccag
     6781 cgccagcacg tcgcggcctt ccagcggaaa gacgggtggc gtttgtgcag ccaggcgctg
     6841 gcgcagagcc gcccattcag atgcgccacc gcccgccagc catgcacgat cgatcagcag
     6901 cgccggttcg gcatcagaca gcagccgccg caacgtcgca tcgtcatctc ccggctgcgg
     6961 cacgggtccg gaccgcagcg cgaccagccg gtcgcgatcc gcagccgaca gccgcagtcg
     7021 cgccgcaacg acggcagcat cgccggtcaa cagcgcggcg agacgcagca ccggttcggc
     7081 aggggctcct gcggcgacca gccgcccgag gccggatggt tcggcgccct cgggcaacac
     7141 agcccccagc actcccagtt cgtccatcag taacaccgcg ccgcgcggat caggtgccgc
     7201 gaggatgcgc gacaactcgc tccacacgcg ctctactgac agtccggtca gcccgtgtaa
     7261 cccgtgccgg atggctgcgg ccgcggacgc gtcgggggtg cccgcgccgt accgcgcgaa
     7321 gaaccggaag aaccgcagga tacgcagaaa gtcctcggca atccgcgtcg caggatcacc
     7381 gacaaacctg atcaacccgg cgcgcagatc cgcgatacca ccgaagtagt catacacagc
     7441 gccatcgcgg ctcatcgaca tcgcattgat ggtgaaatcg cggcgcgccg cgtccgcgcg
     7501 ccagtcatcg gtgaacgcga ccacggcatg acggccatct gtctcgacat cacgccgcag
     7561 ggtggtgatc tcgaagccct ggtgatcgct cactgcggtc accgtgccat gctcgatgcc
     7621 tgtgggcacg gcgcgcagtc gggctgcctg gagcgccttg gttacggctt cgggtgtctg
     7681 tggcgtcgcg agatccactt cggtcaccgc gcggttggcc agcgtatcgc gcactgcacc
     7741 gcccactacg cgggcctccg gcagcgccgc cagcaccgcc gcaagcgccg attcacggag
     7801 aaaggccggc ggaaccagaa tcatcgccgg cgcgtcgctc attgcggcac accgtgcccg
     7861 gccacgatat gtccattttc aaaccgtgcc ggcgcatacg cactgtcacc tgggatcgcg
     7921 cgatcctcgc cgagccacac cagcgctgca gccagaacgg ccaacaggca ggcggccacg
     7981 acgacgaacc ggatcgacgg tcccgactca atcgcgaaga agcgccaggc ggcgaagacg
     8041 atgaatggcg aaaggaacag tgccagttca accagtcgca gcatgcggcc tcgcagcccg
     8101 cagcttctgc gccagatgca ccaggatcgc cgctgtcgcg ccccagatgt aatgatcggg
     8161 atgcggccat acccagaaat cgcgccacat gcctcgcacc tctcggcgat gctgctttgg
     8221 tgcctccggg tccaggagca catcgatcga cagctcgaac acagcctcca cttcgtttgg
     8281 cgctggccgt agatcgaacc cgggctgcag cacgcccagc accggggtga tccggtaccc
     8341 cgtgccggtg acgtggtcgt ccatccgccc cagcaccttc accagcagcg gatccagcgc
     8401 gatctcctcc tcggcctctc gtaaagccgc ggcctcggcg tcggcatcgg tggcgtcgat
     8461 ccggccgccc ggaaagctga cctggccgcc atgcttggcg agatgcgcgc tgcgcttggt
     8521 cagcagaatg cccggtgcgg gtcccaggat gatcggtacc agcaccgcgg ccgggaccgg
     8581 gggcctgtcc aggccgtcca gggcctcggc gccataggtg atctcacccg acaggggagc
     8641 ggctacgatc tcattcagcc gcgcacgcag ctcttcaccg gtcatgccgg gatcataagg
     8701 gacgtcggac tattcttcac cagggggcag ttcgccgagc gaaaagaact tgccgcagct
     8761 ccacacaccc agcatgcgcc ggcagccaac ccattcgggc acacccaacg ctaccagctc
     8821 gtaatacacc gcccggttga tgcgcgcctc gatcccaggc cgcaccagga gatacggggt
     8881 cggctcgcaa gtcaggatgt catgcgccac ccgaagcgga tgctccggcc cggctgtgat
     8941 gacttcatcg acattggtgc ggaaggacag cgcctgatgg cggccgtctc cgctccagtc
     9001 gagctcaacc gcgacgaacg gcacgtcttc cacctcgatg cgcccgcgct ccgcgggtgt
     9061 ctcgagccaa taggtgccat tatcctcgcg ctttaataca ctggcgaaca gacaaaccag
     9121 ctccttgcgc gcgatcggac tgccgcgata cagccaactg ccgtcgcggc ggatcaggaa
     9181 cggcaggtcg ccgcagtcca ccggaggccg gcgcggacgc ttctgccggc aaggtccagc
     9241 cccgaaagtc tccgcaaatg ccacggcagg cgggggcgtc tggtctacca ccatggcgaa
     9301 ttccgcggga gagctggcct cgttcacgtc cgcgtctccg ttactcgaaa actttcatcg
     9361 tgtgtcgcga tataggtagc atccccccac gcgcgaaagt ccctatatga ctggcgagta
     9421 agatttgcag aggtgtaagc aggtatgtcg gtgagtatgg ctaccgcgca acaaggcggc
     9481 ctggacgcca gcgccattct atccgaggtc gaagcgctcg gcgcacgcat cggcaaggtc
     9541 aggactgcga tcggtcgcgt gatcttcggc caggacgaag tggtggatca gaccttgatc
     9601 acgctgcttt ccggtggcca cgcgctgctc gtcggcgttc ctggcctcgg caagagccgt
     9661 ctggtcgaga ccctgggcgt cgtcctcgga ttggcgacca agcgcgtgca gttcacgccg
     9721 gacctgatgc ccgccgacat catcggcagc gaggtcctgg acgaagccga aggccgccgc
     9781 agcttccgct ttgtgccagg gcctgtcttc tgccagttgc taatggccga cgagatcaac
     9841 cgcgccagcc ctcgcaccca atccgcgctg ctgcaggcga tgcaggaaca gcgtgtggcg
     9901 gtgtcgggtg tggaacacgg tctgccgcgc ccgttccacg ttctggcgac gcagaacccg
     9961 atcgagcagg aaggcaccta cccgttgccc gaggcccagc ttgaccgttt cctgctggaa
    10021 gtggacgtgg gttatccggc gctcgatgcc gaacgcacca tgttgctcgc gaccactggc
    10081 gcgccggaag cgcctgcgga acaggcgatg acggccgacg aattgcgcgc cgcgcaggcg
    10141 ctgatccgcc gcgttccggt cggcgaaagc gtggtggatg ccatcctggt gctggtccgc
    10201 agcggccgcc ccgagaccac cgaggatgac atggtccgcc gccacgttgc ctggggtccc
    10261 ggcccccgcg cggcccaggc gctgatgctc gcatcgcgcg cccgcgcac
//
LOCUS       DATGEM010000073         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1576680, whole genome shotgun sequence.
ACCESSION   DATGEM010000073 DATGEM010000000
VERSION     DATGEM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576680"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VKQ27_02445"
     CDS             <1..361
                     /locus_tag="VKQ27_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87822.1"
                     /translation="AKEFVAAFEEKYHYKPDWSANSGYMQMALWADAVTRAGSFYPPD
                     VIKAYEAGKPTQSTVGEVQFRPEDHQLIRPVIIQRGKKKADMKNPDDYYEIVEIAPGG
                     PLMQKPDAFGCHLGDYV"
     gene            402..1454
                     /locus_tag="VKQ27_02450"
     CDS             402..1454
                     /locus_tag="VKQ27_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HLY87823.1"
                     /translation="MPDGLTLRDVEALIAAGLVAPSQHGDVSAVSQRYAVAITPTVRA
                     LIGQVHDPIGLQFVPDPAELRTAAHERADPIADDALSPIKGVVHRYPDRALLKPLLIC
                     PVYCRFCFRREHVGPDGGVLTEAELQAAYDWFAARPAIREVILTGGDPLMLSPRRLGS
                     IVQALSAIPHIEILRVHTRVPTTDPARITGALAATLRTEKAMWMVLHANHAREFSAAA
                     REAIRRIQAEAIPVLGQSVLLRGVNDSPEALEALFRAMLAARVKPYYLHQLDAAPGTA
                     RFHVPIEEGRRLLAALRGRVTGLAWPTYVLDIPGGHGKVPIGPGYLEPDDSVRDVSGI
                     RRALPPQGSYSSPITS"
     gene            complement(1430..2134)
                     /locus_tag="VKQ27_02455"
     CDS             complement(1430..2134)
                     /locus_tag="VKQ27_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLY87824.1"
                     /translation="MITPQSSGSLSGCRLFAGVPSAQLGGLERQCRFRRVARNVRILN
                     QTDETTDVYIVIHGRVRVTMYSRTGREVIFRDFEPGEYFGELAAIDGLTRSATVVALA
                     ETVVASMAAGVFWTALREFPPVQEVVLKRLAGNLRTMNERVLEYSTLGVRNRIHAELL
                     RIARDGRPCETGVIIPLPPTHAELASRVSTHREAVTRELGELARMGLVERRDDGLLIT
                     DVAALERLVQDVMGEL"
     gene            2276..3991
                     /locus_tag="VKQ27_02460"
     CDS             2276..3991
                     /locus_tag="VKQ27_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY87825.1"
                     /translation="MIRAIRRLAAARALRALSPNKIRLISGLTLFTYVGCHLTNHALG
                     NLSVGAMESGLLVQKFIWQGLIGTTALYLALTAHFSLGFWALYQRRHYGWRRAEVAQL
                     ILGLSIPFLLAHHLIVTRVDYSLFGTVKGYSQELYSFWVAAPFWGVVQVTLLNVVWIH
                     GCIGIYYWLRLKPIFRRMAPVLFAGAILLPVLALLGFFQGGRAIRAVAGVAAWRDANL
                     GLRQIGTAGENAWLGWLHQQTLAWLAVLIVAALMARGIRGLKERVAGSIRLFYPQGRI
                     VRVPRGFSILEASLMARVPHASICGGRARCSTCRVRILGDHGDLPAPSETEKSVLYRI
                     HAGPSVRLACQLRPLTDTTVVPLLSATLSIADIFGHATHLGGEERFVAILIIDIRDST
                     QLAARRLPFDAVFAVGNFIDAVSRAVVSAGGRPNQFLGDGLLAIFGLDCDAQEACRRA
                     LTAVCEIAAKVEAINSVFHREWAEPLEFSIGLHGGEAVVGEVAYAGMTVFTALGNPVN
                     VAARMQQHCRTYGCEAVISEDVFALAGIRIEDLPSQIIDLRGIDRALPVRIVDRVAEL
                     AWRLP"
BASE COUNT          732 a         1326 c         1340 g          797 t
ORIGIN      
        1 cgcgaaggaa ttcgtcgcgg cgttcgagga gaagtatcac tacaagcccg actggagcgc
       61 caactcgggc tatatgcaaa tggcgctgtg ggccgatgcg gtgacgcggg ccggcagctt
      121 ctatccgccc gacgtcatca aggcctatga ggcgggcaag ccaacacaat caacggttgg
      181 cgaggtgcaa ttccgccccg aggaccacca attgatccgc cctgtcatca tccagcgcgg
      241 caagaagaag gccgatatga agaacccgga cgactactac gagatcgtcg agatcgcgcc
      301 gggcggcccg ctgatgcaaa agccggacgc tttcggctgc cacctggggg attacgtctg
      361 accctggcgg attggcccta gcaagcctat ttgctggggc catgccggac ggattaacac
      421 tacgggatgt cgaggcgctg atcgctgccg ggcttgtcgc gccgtcacag cacggcgatg
      481 tctcggcggt gtcgcagcgc tacgcggtgg caatcacgcc caccgtgcgg gcgctgatcg
      541 ggcaggtgca tgacccgatc ggcctgcagt tcgtcccgga tccggcggaa ctgcggacgg
      601 cggcgcatga gcgggctgac ccgatcgccg acgatgcgct atcgccgatc aaaggcgtgg
      661 tgcaccgcta tccggaccgt gcgttactga agccgttgct gatctgcccg gtgtattgcc
      721 gcttctgctt tcgccgcgag catgtcgggc cggatggcgg ggtgctgacc gaggcggagt
      781 tgcaggcggc gtatgactgg ttcgctgccc gtccggctat tcgcgaagta attctgaccg
      841 gtggcgatcc gctcatgttg tcgccgcgac ggctcgggtc gatcgtgcag gcgttatcag
      901 ccatcccgca tattgagatc ctgcgtgtac atacgcgcgt cccgacgacc gatcccgcca
      961 ggataaccgg cgcgctggcc gcgacgttgc gcacggagaa ggcgatgtgg atggtgctgc
     1021 atgccaacca cgcgcgggag ttctcggcgg ccgccaggga agcgatccgt cgcatccagg
     1081 ccgaggcaat tcccgtgctc ggccagtccg tgctgctgcg cggcgtgaat gacagccctg
     1141 aggcgctgga ggcgctgttc cgcgccatgc tggcagcgcg ggtgaagccc tactacctgc
     1201 accagctcga tgcagcgcca ggcaccgcgc gcttccatgt gccgatcgaa gaaggccgcc
     1261 gcctgctcgc cgctctgcgc ggccgcgtca ctggtctcgc ctggccgacc tatgtgctgg
     1321 atattcctgg cgggcatggg aaggtgccca tcggcccagg ctatctggaa cccgatgaca
     1381 gtgtgcgaga tgtatcgggc atcaggcgcg cgctaccgcc gcagggatcc tacagctcgc
     1441 ccatcacatc ctgaaccagc cgctccagcg cggcgacatc ggttatcagc aagccgtcat
     1501 cacgccgctc gaccagcccc atgcgcgcga gttcgccaag ctcccgcgtg acagcctcgc
     1561 gatgcgtgct gacccggctc gccagctccg catgcgtcgg cggcaaaggg atgatcacac
     1621 ccgtttcgca cggcctgcca tcgcgtgcga tccgcagcaa ctcggcgtgg atccgattgc
     1681 ggacgccgag cgtgctgtac tcaagcacgc gctcgttcat ggtgcgcaga tttccggcca
     1741 ggcgcttgag tacgacctcc tggacgggag gaaattcgcg cagcgccgtc cagaacaccc
     1801 cggcggccat ggaagccaca actgtttcag ccagggccac aacggtggcg gaccgggtca
     1861 atccatcaat tgccgcgagc tcgccaaaat actcgcccgg ctcgaagtcc ctgaagatca
     1921 cttcccgccc ggtccggctg tacatcgtca cgcggacgcg gccatggatg acgatgtaca
     1981 cgtcggtcgt ttcgtcggtc tgattgagga tgcggacatt gcgtgcgacg cggcggaacc
     2041 ggcattggcg ttccagtccg ccaagctgcg ccgacgggac tcccgcaaac agcctgcagc
     2101 cactgagcga gcccgaagac tgcggcgtga tcatttcgcc ctcattcgcg aaggatgtat
     2161 tccgctactg cccgggaatg gtagcgtcaa ccgaaatgat gaagttcccg acgcgagcgt
     2221 tgcgatatgc cgccgggtcc ggtacggtac gatccggctc agcacaggca gcatcgtgat
     2281 ccgcgccatt cgtcggctgg cagcagcgcg cgcgctacga gcgctgagcc cgaacaagat
     2341 caggctgatc agcggactga ccctgttcac ctatgtcggc tgccatctga ccaatcatgc
     2401 gctgggcaat ctgtcggtgg gcgcgatgga atcgggcttg ctggtccaga aattcatctg
     2461 gcagggattg attggcacga cggcgctcta cctggctttg accgcgcatt tctcactggg
     2521 cttttgggca ttgtaccagc gccggcatta tggctggcgt agagccgaag tggcacaatt
     2581 gatcctcggc ctttcaatac cattcctgct cgcacatcac ctcatcgtca cacgtgtcga
     2641 ctactcgctg tttggcacgg tgaaaggcta ttcacaggag ctgtactcgt tctgggtcgc
     2701 ggcaccgttc tggggtgtcg tgcaggtcac cctgctcaac gtggtgtgga tacacgggtg
     2761 tatcggcatt tattattggc tcaggctcaa gccgatattc cgacgcatgg cccctgtcct
     2821 gttcgccggc gctatccttc tgcccgtcct ggcattactc ggcttcttcc agggcggccg
     2881 ggcgatccgc gcggtggcgg gagtggcggc ctggcgcgac gcgaatctcg ggttgcggca
     2941 aatcggcacg gctggcgaaa acgcctggct cggctggctg caccagcaaa cgcttgcctg
     3001 gcttgcggta ttgatcgtcg ccgcactgat ggcacgggga atccgcggtc tgaaggaacg
     3061 ggtggcggga tcgattcgtc tgttctaccc acagggccgg atcgtgcgag tgccgcgtgg
     3121 cttcagcatt cttgaagcaa gcctgatggc gcgcgtgccg cacgccagta tctgtggtgg
     3181 acgcgcacgg tgctcgactt gccgggtgcg gatactcggc gatcacggtg atctcccggc
     3241 acccagcgag actgagaagt ccgtgctgta taggattcat gcagggcctt ccgtgcggct
     3301 tgcctgccag ctcaggccat tgaccgatac cacggtggtc ccattgctgt cggccactct
     3361 gtcgatagcg gatatcttcg ggcatgcgac acatcttggc ggcgaagagc ggtttgtggc
     3421 gatcctgatc atcgacatac gcgattcaac gcagcttgca gcacgccgcc tgccattcga
     3481 cgccgtgttc gcagtcggga acttcattga cgccgtcagc cgcgccgtcg tctcggccgg
     3541 cggacgcccc aaccagttcc tcggcgatgg attgctcgcc atcttcggac ttgattgcga
     3601 tgcacaggag gcctgccgcc gcgctctcac ggcggtctgc gaaattgccg cgaaggtgga
     3661 ggcgatcaac agcgtctttc accgtgagtg ggcagagcct ctcgaattca gcatcggcct
     3721 gcatggcggc gaggcggtgg tgggcgaagt tgcctatgcc ggaatgacgg tgttcaccgc
     3781 gctcggcaac ccggtcaatg ttgcggcgcg gatgcagcag cactgccgga catacggctg
     3841 cgaggcggtg atctctgaag acgttttcgc cctcgccggc atccgaatcg aagacctccc
     3901 gagccagatc atcgatttgc gcggcatcga ccgtgcattg ccagtccgca tcgtggaccg
     3961 tgtggccgaa ctcgcatggc ggcttccgtg atgccgcctt cataaaggcg tccggcacaa
     4021 cctgttctgc aacgtgagaa agcgcacacc gcgtcggtaa tgcttgctgc tatcccccgc
     4081 gccgcaatga tcccggacgc ggaggaagcc ctcacatggg cggcacgaca cagaacacag
     4141 gcgacgagag cgggggatct gtcccttcac cggtcgtgca atctgttccg gctcc
//
LOCUS       DATGEM010000074         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1114368, whole genome shotgun sequence.
ACCESSION   DATGEM010000074 DATGEM010000000
VERSION     DATGEM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114368"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VKQ27_02465"
     CDS             complement(<1..1462)
                     /locus_tag="VKQ27_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLY87826.1"
                     /translation="MATTTAIARPGHPAGVKAAQVSDQILEKARADTGTVLSELDSRL
                     DGLSQAEADSRLKQYGSNEIAREKRQSALMRLLSNVKNPLVLLLTALGVLSFLTGDLR
                     ATAVIFVMVVLGVVLRFFQEMRADNAAEKLRAMVSNTATLLRDGKQVEVPLKMLVQGD
                     IVRLGAGDMVPADLRVLSAKDLFLNQSALTGESLPVERKADTTSAEVQNPLELPNICF
                     LGSNVESGSATAVVVHTADQTYFGSLAASIVGQRQLTSFDKGVNKFTWLMIWFIAVMV
                     PAVFLINGLSKHDWLEAFLFAMAVAVGLTPEMLPMIVTVNLSKGALAMARKKVIVKRL
                     NAIQNFGAMDVLCTDKTGTLTQGKVVLEKHLDAHGDPSEKVLHYGYLNSYHHTGLKNL
                     LDEAILDHEELEERLKANEKYHKIDEIPFDFVRRRMSVIVEDTTGLNTLICKGAVDEV
                     LDRCTRVEVRGEIIEALPEHDAKRRQIADDLNGQGFR"
     gene            complement(1503..2723)
                     /locus_tag="VKQ27_02470"
     CDS             complement(1503..2723)
                     /locus_tag="VKQ27_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87827.1"
                     /translation="MIAPMLQEAWHAMGANRLRAFLTMLGMMIGVAAVIMMLAIGQGA
                     QYTVNQSIASMGSNLFIILSGSTTSGGLRLGTGATPTLTALDAEAIADLPSIAAVAPV
                     APGGAQLVYGANNWSTVVTGTTPAYLEVRDWNVSSGYAFTDSDVRSGTRVALVGRTVV
                     NNLFGADDPVGRTIRVKQSPFLVIGVLAAKGQSLNGQDQDDTVLIPLTTAQRKVFGSQ
                     FPGSVRFIMAQANSPQAMTAAARDITQLLRQRHHLPERTDNDFDVRNLTALANAQAQT
                     TRVMSIMLGAIASVSLLVGGIGIMNIMLVSVTERTREIGIRVAIGARKRDILTQFLLE
                     AIIISLIGCIVGVLLGVGAAVVANLAAGVTVVITGSAMLLGFAVAAAVGIFFGFYPAN
                     KAARLKPIEALRYQ"
     gene            complement(2720..3439)
                     /locus_tag="VKQ27_02475"
     CDS             complement(2720..3439)
                     /locus_tag="VKQ27_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87828.1"
                     /translation="MSDDVLIVIEHLCKQYDTGAGPVAVLHDVNVIVRRSEFVAIMGP
                     SGSGKTTFMNILGCLDLPTSGKYVLDGRDVGTLDGDALAQLRNAVIGFVFQGYNLLPR
                     ATLVDNVALPLLYAGVAREERARRARELLDKMGLAGFGHYAPSQVSGGQQQRVAIARA
                     MINNPRLILADEPTGNLDTKTSYEIMKLLVQLNAQGITIVLVTHEPDIAAYAKRLVRF
                     VDGRVTHDGSVSQSVIEQIPA"
     gene            complement(3444..>3639)
                     /locus_tag="VKQ27_02480"
     CDS             complement(3444..>3639)
                     /locus_tag="VKQ27_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87829.1"
                     /translation="QGHAVTVYMLAQKVPSGVAVRTGISDGRFTEVLAGSVKVGDPLV
                     IGTMDAVASPSQSTLRTRLF"
BASE COUNT          653 a         1160 c         1120 g          706 t
ORIGIN      
        1 cgcggaatcc ttgaccgttc aggtcgtccg cgatctgccg gcgtttggcg tcgtgctcgg
       61 gcagggcctc gatgatctct ccccttactt ccacccgggt gcaccggtcc agtacttcgt
      121 cgacggcacc cttgcagatc agcgtgttca atccggtcgt atcttcaacg atcaccgaca
      181 tccggcgtcg cacgaaatcg aacgggatct cgtcgatctt gtggtacttc tcgttggcct
      241 tgagtcgttc ctccagctcc tcgtggtcga ggatggcctc gtcgagcaga ttcttcaggc
      301 cggtgtggtg gtagctgttc aggtagccgt agtggagaac tttttcgctg ggatcaccgt
      361 gcgcgtccag gtgcttttcg agcacgacct tgccctgcgt gagcgtgccg gtcttgtcgg
      421 tgcacaacac gtccatcgcg ccgaaattct ggatggcgtt caggcgtttg acgatcacct
      481 tcttgcgcgc cattgccagc gcacccttgg acaggttgac cgtcacgatc attggcagca
      541 tctcgggggt caggccgacg gccaccgcca tggcaaacag gaaagcttcc agccagtcgt
      601 gcttgctcag gccgttgatc aggaagactg ccggaaccat caccgcaatg aaccagatca
      661 tcagccaggt gaacttgttg acgcccttgt cgaagctggt cagctgtcgc tggccgacga
      721 tgctggcagc gagcgatcca aagtaggttt ggtctgccgt gtggacgaca acggccgtcg
      781 cggaaccgct ctccacgttg gagcccagga aacagatatt gggcagttcc agcgggttct
      841 gcacttcggc ggacgtggtg tcggcctttc tctccacggg cagtgattcg ccggtaaggg
      901 ccgactggtt caggaacagg tctttggcgg acagcactcg taggtcggcc gggaccatgt
      961 cgccggcgcc caggcggacg atatcgccct gcaccagcat cttgagcggc acttcgactt
     1021 gcttgccatc gcgcagcagc gtcgccgtgt tgctgaccat cgccctgagt ttttcggcgg
     1081 cgttgtcggc ccgcatttcc tgaaagaaac gcagcacaac gccaagaacc accatcacga
     1141 aaatgaccgc tgtcgcccgc aggtcgccgg tcagaaagga aagcacgccg agcgccgtga
     1201 gcagcagaac caggggattc ttgacgttac tcagcaggcg catcagggcc gattgacgct
     1261 tctcgcgggc aatctcgttc gacccgtact gcttcaggcg cgaatcggct tccgcctggc
     1321 ttaagccgtc caatcgggaa tcaagctcgc tcaggacggt gccggtgtcc gcgcgcgcct
     1381 tttccagtat ttgatcggaa acctgcgctg ccttgacgcc cgccggatga cccggacgcg
     1441 caattgcggt tgtggtggcc actggcaaat tcattcactc ctgcgctgcg cgcccgacgg
     1501 catcattggt agcgtagcgc ttcgattggt ttcagccgtg ccgccttgtt cgcgggatag
     1561 aagccgaaga aaatgccgac cgccgcggcg acggcgaaac cgagcaacat cgcgctcccg
     1621 gtaatgacga cggttacccc ggcggccagg ttggcgacga ccgcggctcc tacacccagc
     1681 aatacaccga cgatgcagcc gatgagtgaa atgatgatcg cttcgagcag gaactgcgtg
     1741 agaatgtcgc gcttgcgtgc gccgatcgcg acccggatgc cgatctcccg cgtacgctcc
     1801 gtcaccgaga ccagcatgat attcatgatg ccgatgccgc cgacaagcag cgacaccgag
     1861 gcaatcgcac cgagcatgat cgacatgacc cgcgtggtct gtgcctgtgc gttggccagg
     1921 gcagtcagat tgcgaacgtc aaaatcattg tctgtgcgct cgggcagatg gtgccgctgg
     1981 cgcagcagct gcgtaatgtc gcgggccgcc gccgtcatcg cctgcggcga attcgcctgt
     2041 gccatgatga agcgcacgct gccgggaaac tgactgccga acaccttgcg ctgcgccgtt
     2101 gtcagcggaa tgagaacggt gtcgtcctga tcctgcccat taaggctctg gcctttggcc
     2161 gcgagcacgc cgatcaccag gaacgggctc tgcttcaccc ggatcgtcct gcccaccgga
     2221 tcgtcggcgc cgaacaggtt gttgaccacc gtgcgcccga ccagggccac tcgcgttccc
     2281 gagcgcacgt ccgagtcggt gaaggcatag cccgatgata cgttccagtc gcggacttcc
     2341 aggtaagccg gcgtcgtccc ggtaaccacc gtgctccagt tattggcgcc gtatacgagc
     2401 tgcgcgccgc cgggcgcgac gggtgccact gcggcgatcg acggcagatc cgcgatcgcc
     2461 tcggcgtcga gggccgtcaa cgtcggagtc gcgcccgtgc ccaggcgcaa gcccccggac
     2521 gtggtcgaac ccgagaggat gatgaacagg ttgctgccca tggacgcgat cgattgattg
     2581 accgtgtact gggcgccctg gccgatcgcc agcatcatga tgacggcggc cacaccgatc
     2641 atcatgccca gcatggtgag gaaagcacgc aatcggttcg cccccatcgc gtgccacgct
     2701 tcctgcagca tcggcgcgat catgcgggaa tctgctctat cacgctctgg gacacgctgc
     2761 cgtcgtgcgt gacacgtcca tcgacaaatc tgaccagccg tttggcataa gcggcgatgt
     2821 ccggctcgtg cgtgaccagc acgatggtaa tgccttgcgc attgagctgg accaacagct
     2881 tcatgatttc atagctcgtc ttcgtgtcca ggttgccggt aggttcgtcg gcgaggatca
     2941 gccgcggatt gttgatcatc gcgcgcgcga tggccacgcg ctgctgctga ccaccggata
     3001 cctggctcgg tgcgtagtgg ccaaagccgg caagtcccat cttgtcgagc agctccctgg
     3061 ccctcctggc gcgttcctcg cgcgctacgc cggcgtagag gagcggcagc gcgacgttgt
     3121 cgacaagggt cgcacgcggc agcaggttgt agccctggaa gacgaagccg atgaccgcgt
     3181 tacgcagttg cgcgagcgca tcaccatcga gcgtgccgac gtcgcggccg tcgaggacgt
     3241 acttgccgct cgtgggcagg tcgaggcagc cgaggatgtt catgaacgtc gtcttgccgg
     3301 agcccgacgg ccccatgatt gccacgaact cgctgcgccg gacgatgacg ttgacgtcat
     3361 gaagcaccgc taccggcccc gcaccggtgt cgtactgctt gcacagatgc tcgatcacga
     3421 tgagcacgtc gtcgctcatc agatcagaac agcctcgttc gcagcgtgct ctggctcggg
     3481 ctggcaaccg cgtccatcgt gccgatcacc agcgggtcgc caactttcac gctaccggcg
     3541 agcacttcgg tgaagcggcc gtcggaaatg ccggttctga ccgcaacgcc acttggaacc
     3601 ttctgcgcca gcatgtagac cgtgacggca tgcccctgc
//
LOCUS       DATGEM010000075         3826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_433075, whole genome shotgun sequence.
ACCESSION   DATGEM010000075 DATGEM010000000
VERSION     DATGEM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433075"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VKQ27_02485"
     CDS             <1..1551
                     /locus_tag="VKQ27_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87830.1"
                     /translation="RYRVLLLEAGPPDTNPWIHIPLGYAKTYVDSAVNWKFETAPQPQ
                     LGNRRLYLPRGKTLGGSSSINGMVYIRGSHGDYDEWRQRGCEGWDWDSVLPFFKKAEN
                     QTRGASEFHGVGGPLNVSDQPAHFELADAVIQACEQAGIPRNPDFNGAIQEGCGYYQT
                     TTSNKRRWSSAKAYLHSAKGRPNLIIQTDAHATRVLIENNRAVGVEFHTAQGRHTARA
                     TGEIVVSGGAYGSPQLLLLSGLGPAQHLQDMGITVVRDMPAVGSNLHDHFNSYVAWRC
                     TRAITLNDLDRSPLRKAVAGVRYALFRSGPMSSNGIHAGVFTRSDPRLERPDIQLNLF
                     EWSTLQRDRDRVIPHPFPGFTLSPVHLRPEGRGTVRLATPDPLAPPEVLFDYLRTEYD
                     MQAMLFGLRLCRRIAEQPALQPYIAHELTPGLSVASDAELADYVRQSGVSNQHPTSSC
                     AMGHGPNTVVDPRLRVHGIAGLRVADASIMPVAVGGNTNAPTIMIGEKAAAMILEDAM
                     AAPRRAAA"
     gene            1517..2677
                     /locus_tag="VKQ27_02490"
     CDS             1517..2677
                     /locus_tag="VKQ27_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY87831.1"
                     /translation="MPWPLHGGPLPDKRAPGKRTMAARPLRVLYLTSVPWLPAQQNHT
                     PGAGPDPFELWQCLSHEGVDVHLMDAARFPLNPLAKLHSFYQSIDPYRSLKVLLARRD
                     YDLVLVSQDGGATIPVLLRRLAGFKAPILIWDLSPAAYWRVRQRIQDQVVPRVDAILA
                     VHSAQAAYVAKRWGPHIPVLTVGYPIDTSFWHPQYSEAPEHILSVGDDPGRDYPTLVA
                     AMDGVDSKLLIRTRHDIAVPPANRSSVGLIASQLDYPTYRSIFARSRFIALPLKPHTL
                     NASGITTLAEAFALGKAAIVTDSDGVRDFLLPGENCLTVPPGDPDALRRAIQRLLREP
                     EECARLGQNARRYAEEHFSRPAFAKRFADILRGFLAASHSTAARSRGNRHGE"
     gene            2667..>3826
                     /locus_tag="VKQ27_02495"
     CDS             2667..>3826
                     /locus_tag="VKQ27_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY87832.1"
                     /translation="MANSADTYDYIVTGAGSAGCVLASRLSESGKHRVLLLEAGGKDT
                     NPWIHIPLGYARTFCNPAVNWMFDSEPEPNLNNRVMYQPRGKVLGGTSSINGMIYMRG
                     NHADYDHWRQLGCEGWDWESVLPYFRKAENNERGSSEFHGAGGPLRVSNQPYEWEIGK
                     LLLQACIEAGIKPNPDFNGADQEGAGYYQTTTNNRRRWSTAAAYLKPARKRQNLTIQT
                     NAHATRVLLENNRAVGVEFRTPQGLRTARAHGEIIVSGGAYGSPQLLLLSGIGPAQHL
                     QDIGITVLHDLPGVGSNLQDHFNTYCAWRISRPLSLNTLHHSIPHQLVAGAKYVLWRG
                     GPMSGNGLYVGALVRSDPALAQPDLQMNISAWSTIDRTRDGIISHLYPAFAI"
BASE COUNT          705 a         1429 c         1053 g          639 t
ORIGIN      
        1 cgctatcgcg tactgctcct ggaagccggc ccgccagata ccaacccctg gatccacatc
       61 cccctcggct acgccaagac ctacgtcgac tcagcagtga actggaagtt cgagaccgcc
      121 ccgcagccgc agctcggcaa ccgacgcctg tatctacctc gtggcaaaac cctcggcggc
      181 tccagctcca tcaacggcat ggtctacatc cgcggcagcc acggcgatta cgacgaatgg
      241 cgccagcgcg gctgcgaggg ctgggactgg gactccgtcc tccccttctt caagaaagcc
      301 gaaaaccaga cccgcggcgc cagcgaattc cacggcgtcg gcggcccgct caacgtctcc
      361 gaccaacccg cgcatttcga actggccgac gccgtgatcc aggcctgcga gcaggccggc
      421 atcccgcgca accccgactt caacggcgca atccaggaag gctgcggcta ctaccagacc
      481 acgaccagca acaagcgccg ctggagctcg gccaaggcgt atctacactc tgcgaagggc
      541 cgcccgaatc tgatcatcca gaccgatgct cacgccaccc gcgtgctgat cgaaaacaac
      601 cgcgcggtcg gggtagaatt ccacaccgcc cagggccgcc acaccgcgcg cgccaccggt
      661 gagatcgttg tctccggcgg tgcctacggc tcgccgcagc ttctgctgct gtccggcctc
      721 ggccccgcgc agcatctgca ggatatgggc atcaccgtcg tgcgtgacat gcccgccgtc
      781 ggctctaacc tgcacgacca tttcaactcc tatgtcgcct ggcgctgcac cagggccatc
      841 acgctgaacg acctcgatcg cagccccttg cgcaaggccg tcgcgggcgt gcgctatgcg
      901 ctgttccgct ccggcccgat gtcgagcaat ggcatccacg ccggcgtttt cacccgcagc
      961 gatccccgtc tcgagcgtcc cgacatccag ctcaacctgt tcgaatggag cacgctccag
     1021 cgcgacaggg atcgcgtgat cccgcatccc ttccccggct tcacgctgag ccccgtgcat
     1081 ctgcgccctg aaggccgagg caccgtgcgc ctcgccaccc ctgatccgct ggcacccccc
     1141 gaggtgctgt tcgactacct gcgcaccgaa tacgacatgc aggccatgct gttcggcctt
     1201 cgcctctgcc gcaggatcgc cgaacaaccc gcactgcagc cctacatcgc gcacgagctc
     1261 acgcctggtc tttcggtcgc cagcgatgca gaactcgccg actacgtgcg ccagagcggc
     1321 gtttccaacc agcatcccac gagttcctgc gccatgggcc acggccccaa caccgtggtc
     1381 gatccacgcc tgcgggtcca tgggatcgcg ggactgcgcg tcgccgatgc ctcgatcatg
     1441 cccgtcgccg tcggcggcaa caccaacgcg ccgacaatca tgatcggtga gaaagccgcc
     1501 gcgatgatcc tggaagatgc catggccgct ccacggcggg ccgctgcctg ataagcgcgc
     1561 ccccggcaag cgcaccatgg cggctcgccc gcttcgcgta ttgtacctga catccgtccc
     1621 ctggctcccc gcgcagcaga accatacgcc cggcgcggga cctgaccctt tcgaactgtg
     1681 gcagtgctta tcgcacgagg gtgtggacgt gcatctgatg gacgcggcac ggtttccgct
     1741 gaacccgctg gcgaagctgc attccttcta ccaatcgatc gatccgtacc gttccctgaa
     1801 agtcctgttg gcacgacgcg attatgatct ggttctcgtc agccaggacg gtggagcaac
     1861 aatccccgtt ctgctgcggc gcctcgccgg cttcaaggct ccaattctta tctgggacct
     1921 gtcgcccgcc gcctattggc gtgtcaggca gcgcattcag gaccaggttg tcccacgggt
     1981 cgacgcaatt cttgctgtcc attccgccca ggctgcgtat gtcgcaaaac gctggggccc
     2041 gcacataccc gtgctgaccg tcggatatcc gatcgatacc agcttctggc atccgcagta
     2101 cagtgaagcg ccggagcaca tcctttccgt cggcgacgat ccggggcgcg actacccgac
     2161 attggtcgcc gcgatggatg gcgtcgacag caagctgctg atccggaccc gccacgacat
     2221 cgcggttcca ccggccaacc gttcctcagt cggactcatc gcctcccaac tggattaccc
     2281 aacctaccgc agcatatttg cgcgcagccg gttcatcgcg ctgccgctga agccccatac
     2341 gttgaatgcc agcggcatca ccacgctcgc cgaggctttc gcactgggca aggcggccat
     2401 cgttacggac tccgacggcg tgcgggactt cctgctgccc ggtgaaaatt gcctgaccgt
     2461 gccgcctggc gatcccgatg cgctgcgccg cgcgatacag cgcctgctgc gcgaaccgga
     2521 agaatgcgcc cggctcggcc agaacgcgcg acgctacgcc gaagagcact tctcacgacc
     2581 ggcattcgcc aaacgcttcg ctgacatatt gcgcggattt cttgcagcgt cacactcgac
     2641 agctgctcga agcaggggga atcgacatgg cgaatagcgc cgacacctac gactacatcg
     2701 tcaccggcgc aggttccgcc ggctgcgtgc tggcctctcg cctctccgag tccggcaagc
     2761 atcgcgtgct gctgctcgaa gccggcggca aggacaccaa cccctggatc cacatcccgc
     2821 tcggctacgc ccgcaccttc tgcaatcccg ccgtcaactg gatgttcgac agcgagcccg
     2881 agcccaacct caacaaccgc gtcatgtacc agccgcgcgg caaggtgctc ggcggcacca
     2941 gctcgatcaa cggaatgatc tacatgcgcg gcaaccacgc cgactacgac cactggcgcc
     3001 agctcggctg cgagggctgg gactgggaat ccgtcctccc ctatttccgc aaggccgaga
     3061 acaacgaacg cggctcctct gaattccacg gcgccggtgg cccgttgcgc gtctccaacc
     3121 agccctacga gtgggaaatc ggcaagctgc tgctgcaggc ctgcatcgag gccggcatca
     3181 aacccaaccc cgacttcaac ggcgccgacc aggaaggcgc cggctactac cagaccacca
     3241 ccaacaaccg ccgccgctgg agcaccgccg ctgcctacct gaagcccgcg cgcaagcgcc
     3301 agaacctcac catacagacg aacgcccatg ccacccgcgt gctgctggaa aacaaccgag
     3361 ccgtcggcgt cgaattccgc actccccaag gcctgcgcac cgcccgcgcc catggcgaaa
     3421 taatcgtctc gggcggtgca tacggttccc cgcaactcct gttgctctcc ggcatcggcc
     3481 ccgcacagca tttgcaggac atcggcatca ccgttctgca cgatctgcct ggtgtcggct
     3541 cgaatctgca agaccacttc aatacctact gtgcctggcg catctcgcgc cctctttcat
     3601 tgaacacgct gcaccacagc atcccacacc aactcgttgc cggtgcgaag tatgtgctgt
     3661 ggcgaggcgg cccgatgtcc ggcaacggcc tctatgtcgg cgccctggtc cgcagtgatc
     3721 ccgcgctggc ccagcccgac ctgcaaatga acatctccgc ctggagcact atcgatcgca
     3781 cgcgcgacgg catcatctcg cacctctacc ccgcctttgc gatcag
//
LOCUS       DATGEM010000076         8943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1575314, whole genome shotgun sequence.
ACCESSION   DATGEM010000076 DATGEM010000000
VERSION     DATGEM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575314"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VKQ27_02500"
     CDS             <1..1304
                     /locus_tag="VKQ27_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87833.1"
                     /translation="LATGYEWADEKTLVIHMRPGVKFHDGEPVDAEAVKYTLNRDLTL
                     QGSFRRSEISSIDRVDIVDQATVRIVLKTPSGAFLAQLTDRSGMPLPPKATEQAGKDF
                     AAHPVCSGPFKFVERAAQDHVTLEKFADYWDAKDIHFDKVIYQVFTDSSVRLANLKAG
                     TSDIVEFIAPTDTAAVKADTKLRLVVFDALGNLGITNNLDNGARADTPYGKNALVRQA
                     FSAAIDRTALVNVVYSDMYAQSVQPVSASSPFYDPSLKPPPRDVAKAKALLKQAGVTL
                     PVKVDLMTPNQPDQLQVAEVIQSMTSEAGFDVHIQAVEFASSLQASARGDYQAYLIGW
                     SGRVDADGNSYAFLHSGQGNNASHYGNPTVDALLDDARGSTDLAKRKADYFKVWAQIQ
                     QDLPITYLYNPRNIMAMSVKVKGFRPVPDGMVRLQGLEMSK"
     gene            1304..2251
                     /locus_tag="VKQ27_02505"
     CDS             1304..2251
                     /locus_tag="VKQ27_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87834.1"
                     /translation="MRGHLGARIAQIIPTLLLVSVLVFCLQQLMPGDPALVMAGEERG
                     DPQVLAQIRAELWLDRPLPVQYMHWMGNVVRGNLGYSWRIREPVTQLIAEKLPVTLQL
                     SVMAFAIALLIGVPTGVIAAVYRNRWLDYVANAVGLAGLSTPTFWLGIMLILLVSVNL
                     GWLPPSGYVSLAEDWRQSLATTIMPAFVLGNAIAAILMRHTRSAMLTSLDQDYVRTAR
                     AKGLREFVVVIRHALRNALIPVVTLGALELGTLFSGAVLTEQVFSIPGFGKMIVDAVF
                     NRDYPVVQGVVLVTAFLYVVLNLLADVLYVVINPRLRGA"
     gene            complement(2345..2926)
                     /locus_tag="VKQ27_02510"
     CDS             complement(2345..2926)
                     /locus_tag="VKQ27_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY87835.1"
                     /translation="MLTLYFAPGASSMAPHIALHEVGAAYEPKPLSFHKKQNRDPAFL
                     AINPEGKVPTLLIDDRVLTEVAAILWYLGRRYPEARLLPTNDPETEAQVVSWMSFIAA
                     TLHPARRQGPEHARLVYSIAEQRLGNRDWAVGQYSIADIHLFRLFWRFRGSLNPAPGD
                     FPALTAHHDRMMARPAVSKTIEIESAIGYELPA"
     gene            complement(2920..3723)
                     /locus_tag="VKQ27_02515"
     CDS             complement(2920..3723)
                     /locus_tag="VKQ27_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HLY87836.1"
                     /translation="MDGTATPKHYDAHSVFRPENLLREARRQRGLPQSPVPAVCALDP
                     DGDIVRALRVNGRSRRADAWACYHTDMDVTEHKGITIGIVGLAVGAPFAVLVAEQLFV
                     SGCRLLVSITSAGQITPIVPPPYFVLIDRALRDEGTSHHYLPPSTFADAEPALINRVW
                     PSLTALQERVHRGTTWTTDAPYRETQQAIETARTHGALAVEMEAAALYAFAKARHRAV
                     LCFAHVTNQMAVVEGDFEKGEADGAHSSLDVIAATARACGFALTGDAQC"
     gene            3827..4429
                     /locus_tag="VKQ27_02520"
     CDS             3827..4429
                     /locus_tag="VKQ27_02520"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLY87837.1"
                     /translation="MRPNPLNDTVHFLIQPAWFTPVFWVLLAASIVVALLVWARDPMQ
                     RAPRDVGLWVLRVLIGSMWWQQSLWKIPPNFDGLKYWMGQEAEHAAIVLQGNLVRDVV
                     LPNLSLFGPLVYLIEVAIGVSLLLGVFSRAGAALGLLMGLNLWLGLYSAPGEWPWTYM
                     FLSVIQALFVLDPPGRVLGVDALLRRRTAGRVPAGLVWVS"
     gene            4430..5149
                     /locus_tag="VKQ27_02525"
     CDS             4430..5149
                     /locus_tag="VKQ27_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLY87838.1"
                     /translation="MATFLMIGRRALPLLAFAAAARPAAAATIDLSLACDTTLAPALR
                     KVAAAYAAKTGVRVFVFPTGPGLLLPQLERNIQNDILVTQLPILEQALQGKIIAAMPG
                     APRWSNPLVIAGRSAASVNGVFAACDATAASDFDGPALLAKLELKPAQVMGAVDTDEV
                     AWLLLRGEAQAGFLHMTDVRAHPELTVIQNVPPDVQAPFVYGATVTKLASRPDPQGFI
                     AFMQTPEAMTLLKEAGLEVQS"
     gene            5146..5820
                     /locus_tag="VKQ27_02530"
     CDS             5146..5820
                     /locus_tag="VKQ27_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLY87839.1"
                     /translation="MTKVYDEQKHPLSRRIFHWIMAISILLMIGSGWRIYDASPIFPF
                     TFPDWATLGGDVWTSLARHGDPGVASAIAWHFAAMWTLFASYILFLLWGLVSGHFRRD
                     FLPVGPRSILRDFVAAVRFRLDHRLGEYNAVQKIAYIGVLGLIALMLISGLAIWKPVQ
                     TYPLVALFGGFQGARLVHFLGMAGIVLFLVVHVALTFLVPRTLLAMVWGRAREPRNLV
                     TSEDVP"
     gene            5817..6521
                     /locus_tag="VKQ27_02535"
     CDS             5817..6521
                     /locus_tag="VKQ27_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY87840.1"
                     /translation="MIVARRGLLRGGVSLGALTLLTGCDISDDNAVQSALAVFSRWND
                     RVQAALFGEQRLAQTFPESEAVKDFRYNAWYGPDKAPRLNSADYRLKLAGRIENKRPW
                     TVEELYALPQASQVTRHVCVEGWSMIGKWSGTPLRTFLERVGADTSARYVGFECADGY
                     YEGIDMPTALHPQTLMAFRLSDEILPTKYGYPFKVRIPTKLGFKNPKFVTAIYVTNDQ
                     PRGYWTDRGYNWFSGI"
     gene            6526..7263
                     /locus_tag="VKQ27_02540"
     CDS             6526..7263
                     /locus_tag="VKQ27_02540"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HLY87841.1"
                     /translation="MERRGVCAMGVMAKAPQPGKAKTRLCPPLLPEQAARLSAAFLRD
                     ITENIVAAARDSPIDGCIAYAPMGTEAWFDGHLADGTGFVLADGSPPMAENVQGFGRC
                     LLHAVQAMLGSGYGAACVVNSDSPTLPTAVLVEAARVLLAPGERVVLGAADDGGYYLL
                     GMKQAYDHLFADIVWSTDTVAAVTRARAAELGLPVVELPMWYDVDDASALARLRAELS
                     ASADVAPATRACLERIGSSDIVPIAAQ"
     gene            7260..8588
                     /locus_tag="VKQ27_02545"
     CDS             7260..8588
                     /locus_tag="VKQ27_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HLY87842.1"
                     /translation="MSGETRRAVLLVAIGGMLLVLVPAGLAALLLADGASVPLFMTGI
                     CLAGALWLGAVALVRYGALPHWMVWFVVGVAVAMRAMTLAAPPLLSTDVYRYVWDGRV
                     QMAGINPYRYLPAAPELASLRDDAVYPSINRAGYAPTVYPPAAEGVFALAVAVAPGVF
                     GMKLMMLGFDALALVALAWLLRVAGRPAAELLIFAWLPLPVWEFAGNAHVDAVTAGVL
                     TLALVVAAQGRAVWTGIMLAVATLTKFLPAVVVPAFWRPRDWRLPFAFAVAVMVLYIP
                     YLSVGWRVFGFLGGYVSEEGVQSGHGIFLLQLLGSAVTLPSWAGAAYIAGALAVVGAL
                     AARFAFSGTLPAAAGPRVLLIARQAAILGAAVLVAVSPHYPWYFAWLAPLACLAPIPS
                     ILWMLAAAPLLAHGAVETLIVPGAVYIPAAALALRDLRRLPIPQPVIRSA"
     gene            8588..>8943
                     /locus_tag="VKQ27_02550"
     CDS             8588..>8943
                     /locus_tag="VKQ27_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLY87843.1"
                     /translation="MTATTLKDPRRYFEAVEARQTEATAPPVCLYLEVTNRCNLLCET
                     CPRTFETLEPPADMSWELFTRIVDQVPEVARVVLHGVGEPMMVKALPRMIRYLKDRGV
                     YVLFNTNGTLLTPKKH"
BASE COUNT         1414 a         2362 c         3346 g         1821 t
ORIGIN      
        1 agctggccac aggatatgaa tgggcggacg agaagacgct ggtgatccac atgcggccgg
       61 gcgtgaagtt ccacgatggc gagccggtgg atgcggaggc ggtgaagtac acgctgaacc
      121 gcgatctgac gctgcaggga agcttccgcc ggagcgagat cagttcgatt gaccgtgtgg
      181 atatcgttga tcaggcgacg gtgcggatcg tgcttaagac gccgtcgggg gcgttccttg
      241 cgcagttgac cgatcgttcc ggcatgccgc tgccgccgaa ggcgaccgag caggcgggga
      301 aggatttcgc agcgcacccg gtgtgcagcg gaccgttcaa gtttgtcgag cgtgcggcgc
      361 aggatcacgt gacgctcgag aagttcgcgg actactggga cgcgaaggac attcatttcg
      421 ataaggtgat ctatcaggtc ttcacggatt cctcggtgcg gctggcgaac ctgaaagcgg
      481 gaacgagcga catcgtcgag ttcatcgcgc caacggatac ggcggcggtg aaggccgata
      541 cgaagctgcg gctggtggtg ttcgatgcgt tggggaattt ggggatcacc aacaacctgg
      601 acaacggcgc gcgcgcggat acgccgtatg gcaagaatgc gctggtgcgg caggctttct
      661 ccgccgcgat cgaccggacg gcgctggtga acgtggtgta tagcgatatg tatgcacaga
      721 gcgtgcagcc ggtttcggcg agctcgccat tctatgatcc gtcgctgaag ccgccgccgc
      781 gtgatgtggc gaaggcgaag gcattgctga agcaggcggg ggtgacgctg ccggtaaagg
      841 tggatctgat gacgccgaac cagccggacc agttgcaggt ggcggaggtg atccagtcga
      901 tgacgtcaga ggcggggttc gacgtgcata tacaggcggt cgagttcgcc tcgtcattgc
      961 aggcgtcggc gcgaggagat tatcaggcct acctgatcgg ctggtcgggg cgcgtggacg
     1021 ccgatggcaa ttcgtatgcg ttcctgcatt cggggcaggg gaataacgcc agccactacg
     1081 gcaatccgac ggtggatgca ctgctggatg acgcgcgcgg ctcgacggac ctggcgaagc
     1141 ggaaggccga ttacttcaag gtctgggcgc agatacagca ggatttgccg atcacgtacc
     1201 tgtataatcc tcggaacatc atggcgatgt cggtgaaggt gaaggggttc cggccggtgc
     1261 ctgacgggat ggtcaggctg caaggtctgg agatgtcgaa gtagtgcgcg ggcatctggg
     1321 cgcgcgtatc gcgcagatta tcccgacgct gctgctggtc agcgtgctgg tcttctgttt
     1381 gcagcagttg atgccgggcg atccggcgct ggtgatggcg ggggaggagc gcggggatcc
     1441 tcaggtgctg gcgcagatcc gcgccgagct ttggctcgat cggcctttgc cggtgcaata
     1501 catgcactgg atggggaatg tggtgcgggg gaacctgggc tactcgtggc gcatccgcga
     1561 gccggtgacg cagttgatcg ccgagaagct gccggtgacg ttgcagctga gcgtgatggc
     1621 gttcgcgatt gccttgctga ttggcgtgcc gacgggggtg atcgcggcgg tctatcgtaa
     1681 ccgatggctc gattacgtgg cgaatgcggt gggactggcg gggttatcga cgccgacgtt
     1741 ctggttgggg atcatgctga tcctgctggt gtcggtgaat ctggggtggt tgccgccatc
     1801 gggctacgtg tcgctggccg aggattggcg acagtcgctg gcgacgacga tcatgccggc
     1861 gttcgtgctg ggcaacgcga tcgcggcgat cctgatgcgg catacgcgga gcgcgatgct
     1921 gacgtcgctg gatcaggatt atgtgcggac ggcgcgggcg aaggggcttc gcgagttcgt
     1981 cgtggtgata cggcacgcgt tgcgcaatgc gctgataccg gtggtgacgt tgggagcgct
     2041 ggagcttggg acgctgttct cgggggcggt gctgacggag caggtgttca gcattccggg
     2101 gttcggcaag atgatcgtcg atgcggtgtt caatcgcgat tacccggtgg tgcagggcgt
     2161 ggtgctggtg accgcgttct tgtacgtggt gctgaatttg ctggcggacg tgctttacgt
     2221 ggtgatcaat ccgaggttgc gaggcgcgta gggctggtga tctgcagtca ttgcgagtga
     2281 agcgaagcaa tctggtcgag tttgcttcgc ttcgctcgca atgacatgtg ccggggacac
     2341 agttttatgc gggcagttcg tagccgatgg cggattcgat ctcgatcgtc ttgctgacgg
     2401 cggggcgggc catcatccgg tcgtggtggg cggtgagagc ggggaagtcg ccgggggcgg
     2461 ggttcaggga gcctcggaaa cgccagaaca gacggaagag gtggatgtcg gcaatggagt
     2521 actggccgac tgcccagtcg cgattgccca ggcgctgctc ggcgattgaa tatacgagcc
     2581 gggcgtgctc cgggccctgg cggcgggcgg gatgcagcgt cgcggcgatg aacgacatcc
     2641 acgagacgac ctgggcctcg gtttccgggt cgttggtagg aaggagcctg gcctccggat
     2701 agcggcggcc gaggtaccag aggattgcag caacctcggt cagcacacgg tcgtcgatca
     2761 ggagcgtggg aaccttgccc tcgggattga ttgccaggaa ggcgggatcg cggttctgct
     2821 tcttgtgaaa cgagagcggc ttgggttcgt aagctgcgcc gacttcgtgc agcgcgatgt
     2881 ggggagccat cgaactggcg ccgggggcga aataaagcgt cagcattgag catctccagt
     2941 cagggcgaag ccacaggcgc gcgcggtggc ggcgatcacg tcaagcgaac tgtgggcgcc
     3001 atcagcttcg cctttttcga agtcaccttc cacgacggcc atctgattcg tcacgtgggc
     3061 gaagcatagc acggcgcgat ggcgggcctt ggcgaaggcg tagagggccg cggcttccat
     3121 ttcgacggcg agggcgccgt gagtgcgggc ggtttctatc gcctgttggg tctcgcggta
     3181 gggtgcatcg gtggtccagg ttgtgcctcg atgcacgcgt tcttgcaggg cggtgaggga
     3241 tggccagaca cgattgatca gcgcgggttc ggcgtccgcg aacgtcgagg gtggcaggta
     3301 gtggtggctg gtaccctcat cgcgcagggc ccggtcgatc aggacgaaat agggcggggg
     3361 gacgatcggg gtgatctgtc cggctgacgt gatactgacg aggaggcggc agccagacac
     3421 gaagagctgc tcggcgacca gcacggcgaa aggggcaccg acggcgaggc cgacgatgcc
     3481 gatggtgatg cctttgtgct cggtgacgtc catatcggtg tggtagcagg cccaggcatc
     3541 ggcgcgccgc gatcggccat tgacacgcag ggcgcggacg atgtcgccat ctgggtccag
     3601 ggcgcagacg gctggcacgg gagactgggg gaggccacgc tggcggcggg cctcgcgcaa
     3661 caggttctcg ggccggaaca cggagtgggc atcgtaatgc ttcggtgtgg cggttccgtc
     3721 cattgcgttc ttcgcccttt gcgtgatcag ggttgtaacg cttggccatg tcgcaagacc
     3781 actatccggg tagcggcgcg gccgatgccg agaggagagc aaggctatgc ggccgaaccc
     3841 gctgaacgat accgtccatt ttttgatcca gccggcctgg ttcacgccgg tgttctgggt
     3901 gctgctggcg gcgagcattg tggtcgccct actcgtgtgg gcgcgcgatc cgatgcaacg
     3961 cgcgccgcgt gacgtcgggc tttgggtgct gcgtgtgttg attggctcga tgtggtggca
     4021 gcagagcctg tggaagatcc cacctaactt cgatgggctg aaatattgga tgggacagga
     4081 agccgagcac gccgcgatcg tgcttcaggg taatttggtg cgcgacgtcg tattgccgaa
     4141 tctgagcctg ttcgggccgc tggtgtatct gatcgaagtc gccatcggcg tgtcgctgtt
     4201 gttgggcgtg ttctcgcggg cgggcgcggc gctggggctg ctgatggggt tgaacctctg
     4261 gctggggttg tattcggcac ctggcgaatg gccgtggacc tatatgttcc tgagcgtgat
     4321 ccaggcgttg ttcgtgctgg atcctccggg gcgcgtgctg ggcgtcgacg ccttgctgcg
     4381 gcggcgaacg gcgggtcggg ttcctgctgg actggtctgg gtgagctgaa tggccacatt
     4441 tctcatgatt ggacggcgcg cgctgccttt gctggcgttt gccgcggcgg cgcggccggc
     4501 ggctgcggcc accatcgatc tgtctttggc ctgcgacacc acgctggcgc cggcgctgcg
     4561 caaggtcgcg gcggcctatg cggcaaagac gggcgtgcgg gtgttcgtgt tcccgacggg
     4621 gcccgggctg ctgctgccgc agctcgagcg gaacatccag aacgacatcc tggtgacgca
     4681 gctgccgata ctggagcagg cgttgcaggg gaagatcata gcggcgatgc cgggcgcgcc
     4741 gcgctggagc aatccgctgg tgattgcggg gcgcagcgca gcctcggtaa atggcgtgtt
     4801 cgctgcgtgt gatgcgacgg cggcgtccga tttcgatggg ccggcgttgt tggcgaagct
     4861 ggagttgaag ccggcgcagg tgatgggcgc tgtggatacg gacgaagtgg cgtggttgct
     4921 gctgcgcggc gaggctcagg cgggttttct gcatatgaca gacgtacgcg cgcatccgga
     4981 gctgaccgtg atccagaatg tcccgcccga tgtgcaggcg ccgttcgtct atggcgccac
     5041 ggtcacgaag ctggcgtcac ggcctgatcc acaagggttc atcgcattca tgcagacgcc
     5101 ggaggcgatg acattgctga aagaggccgg gctggaggtg cagtcatgac caaggtttac
     5161 gacgaacaga agcatccgct gtcgcggcgc atcttccatt ggatcatggc gatttccatc
     5221 ctgctcatga tcggcagtgg ctggcgtata tacgacgcga gcccgatttt cccgttcacg
     5281 ttccccgact gggcgacgct gggcggcgat gtatggacgt cactggcgcg gcatggcgat
     5341 ccaggcgtgg cgtccgcgat cgcctggcat ttcgcggcga tgtggacgct gttcgcgagc
     5401 tatatcctgt tcttgctgtg gggactcgta tccggacatt tccggcgcga tttcctgccg
     5461 gtgggaccgc ggtcgatcct gcgcgacttc gttgccgcgg tgcggttccg gctggatcat
     5521 cggctgggcg aatacaacgc agtccagaag atcgcgtata tcggcgtgct gggactgatt
     5581 gcgctgatgc tgatctcggg gctggcgatc tggaagccgg tgcagaccta tccgctggtg
     5641 gcgttgttcg gtgggttcca gggcgcccgg ctggtgcact tcctgggaat ggcggggatc
     5701 gtgctgttcc tggtggtgca cgtcgcgttg acgttcctgg tgccgcgcac gctgctggcg
     5761 atggtctggg ggcgcgcgag ggagcctcgc aatcttgtta cttcagagga tgtaccatga
     5821 ttgtcgcaag acgtgggctg ctgcgtggcg gtgtttcgct cggtgcgctg accctgctga
     5881 cggggtgcga catcagtgac gacaacgcgg tgcagagcgc gctggcagtc ttttcgcgtt
     5941 ggaatgatcg ggtgcaggcg gcgctgttcg gcgaacaacg gctggcccag acatttccgg
     6001 aatcggaggc cgtgaaggac ttccgataca atgcgtggta cgggccggac aaggcgcctc
     6061 gccttaattc cgccgattat cgcctgaaac tggcagggcg gatcgagaac aagcgaccgt
     6121 ggactgtcga ggagctgtat gcgctgcctc aggcaagcca ggtgacgcgc catgtctgcg
     6181 tggaaggctg gagcatgatc ggcaaatgga gcggcacacc gttgcgcacg ttcctggagc
     6241 gcgtcggcgc cgataccagt gcgcgttacg tcgggttcga gtgtgcggat gggtactatg
     6301 aggggatcga catgccgacg gcgctgcatc cccagacgct gatggcattc cgtctgtcgg
     6361 acgagatatt gccgacgaaa tatggctatc cgttcaaggt gcggataccg acgaaactgg
     6421 ggttcaagaa cccgaaattt gtcacggcaa tttatgttac caacgaccag ccgcgcgggt
     6481 attggacgga tcgcgggtac aactggttca gcgggatctg agcgcatgga gcgacgcggt
     6541 gtctgcgcga tgggcgtgat ggcgaaggca ccgcagcctg gcaaggcgaa gacacgtttg
     6601 tgcccgcccc tgctgcccga gcaggcggcg cggctgagcg ctgcattcct gcgggatatc
     6661 accgagaaca tcgtagcggc tgcgcgggat tcgccgatcg atgggtgcat tgcctatgcg
     6721 ccgatgggaa cggaggcgtg gttcgacggg catctggcgg atggcaccgg attcgtgttg
     6781 gccgatggat caccgccaat ggcagagaat gtgcaggggt ttggacgatg cctgctgcat
     6841 gcggtgcagg cgatgcttgg atcggggtat ggggctgcgt gcgtggtgaa ctccgacagc
     6901 ccaacattgc cgacagccgt gttggtggag gcggcgcgcg tgctgctggc cccaggagag
     6961 cgggttgtgc ttggtgcggc ggatgacggc ggatattacc tgctgggtat gaagcaggcg
     7021 tacgatcatc tgtttgccga tattgtctgg agcacggaca cggtggccgc ggtgacgcgt
     7081 gcgcgagcgg cggagctggg gctgccggtg gtggaattgc cgatgtggta cgacgtggat
     7141 gatgcgtcgg cgctggctcg attgcgggcg gagctgtctg cgtcggcgga tgtggcgccg
     7201 gcgacgcggg cctgcctgga gcgtatcggc agcagcgata tagttcccat cgcggcgcaa
     7261 tgagcggaga aacccgccga gctgttctgc tggtggcgat tggcggaatg ctgctcgtgc
     7321 tcgttccggc ggggcttgcg gcgctgctgc tcgcagacgg ggcaagcgtg ccgttgttca
     7381 tgaccggcat ttgtctggct ggtgcactgt ggctcggggc ggtggcactg gtgcggtacg
     7441 gtgctttgcc acattggatg gtgtggttcg tcgttggcgt tgcggtggcg atgcgagcga
     7501 tgacgctggc agcgccgccg ctgctgtcca ccgatgttta tcgctatgtc tgggatggcc
     7561 gcgtgcaaat ggcggggatc aatccgtatc gctatctgcc ggcagctcct gaattggcgt
     7621 cgctgcgcga cgacgcggtg tatccgagca tcaatcgcgc cggctatgca cctacggtgt
     7681 atccgccagc ggcggaaggg gtgtttgcgc tggccgtcgc ggttgcgcct ggcgtgttcg
     7741 gcatgaagct gatgatgttg ggctttgatg cgctggcact tgtcgcgctg gcctggctgc
     7801 tccgcgtggc gggccggccg gcggcggaat tgctgatctt cgcgtggctg ccgctgccgg
     7861 tatgggagtt tgccggcaac gcgcatgtcg atgcggtgac cgcgggcgtt ctgacattgg
     7921 cgttggttgt cgctgcgcag ggacgggcgg tatggaccgg gattatgctg gcggtcgcga
     7981 cgctgaccaa attcctgccg gccgtggtgg tgccggcgtt ctggcgaccg cgcgactggc
     8041 ggctgccgtt cgcgttcgcc gtggccgtga tggttttgta tataccgtat ctgtcggtcg
     8101 ggtggcgggt gttcggattc cttggtgggt atgtctcgga agagggtgtg cagagcggcc
     8161 atggcatctt cctgctgcaa ctgctgggca gcgcggtgac gctgccatcg tgggcaggtg
     8221 cggcgtatat cgctggggca ctggcggtgg taggagcgtt ggcggcacgc tttgctttca
     8281 gcgggacgct gccggctgcg gctggaccgc gcgtgttgct gattgcacgg caggcagcga
     8341 tcctgggcgc tgccgtgttg gtggcggttt caccgcatta cccctggtat ttcgcctggc
     8401 tggcaccgct ggcctgtctg gcaccaattc cgagcatcct gtggatgctc gccgcggcgc
     8461 cactgctggc gcacggcgcc gtggagacgc tgatcgtgcc tggcgccgtg tatatacccg
     8521 ctgcggcgct tgcattgcgt gacctgcgcc gcctgccgat acctcaaccc gtgatacgga
     8581 gcgcctgatg accgcgacga cgctgaagga tccgcgccgc tatttcgagg ccgtggaggc
     8641 gcgccagacg gaggcgacag caccaccagt gtgcctttac ctggaggtga cgaaccgctg
     8701 caatctgctg tgcgagacat gtccccggac tttcgagacc ctggagccgc cggcggatat
     8761 gagctgggaa ctgttcacgc gcatcgtgga ccaggtgccg gaagtggcac gcgtggtgct
     8821 gcacggcgtt ggtgagccga tgatggtgaa ggcgctgcca cggatgatcc ggtatctgaa
     8881 ggatcgcggt gtgtatgtgc tgttcaatac gaatggcacg ctgctgacgc cgaagaagca
     8941 cca
//
LOCUS       DATGEM010000077         6741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1003057, whole genome shotgun sequence.
ACCESSION   DATGEM010000077 DATGEM010000000
VERSION     DATGEM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003057"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..962)
                     /locus_tag="VKQ27_02555"
     CDS             complement(315..962)
                     /locus_tag="VKQ27_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HLY87844.1"
                     /translation="MRIDPAEAAHLILAGQALPPEWIPHITDLRLERSYLSDLAPLSA
                     LTHLRQLHLTRSRISDIAPLSPLVHMQSLHLSFTAVRDLSPLAHMRELQACFLTGTLV
                     TDIAPLTGLTRLERIDLGDTPIIDASPLSNLPNLHCLDLSNTTIRDITALAKLTALEY
                     LDLTNTKVTDLTPLAALTNLRRIDLRGVSADPAALARLPHCKIVTVATPPRRRRH"
     gene            complement(952..1788)
                     /locus_tag="VKQ27_02560"
     CDS             complement(952..1788)
                     /locus_tag="VKQ27_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY87845.1"
                     /translation="MPYETIEVKPVTAVIGAEVFGIDLGKPLGNQQFQEVHDALMAHQ
                     VLFFRDQKLTLDQHKAFGRLFGELHIHPNTPGPEGHPEILPIYADANSKRINGERWHS
                     DVSCDPEPPMGSILNLKVVPPVGGDTIFSSMYAAYDALSEPMKQYLSQLTAIHDGSMN
                     YTRTNNLIGRDGPKTFPRAEHPVIRTHPVSGRQAIFVNPVFTHSIKDVPEGESKAILD
                     FLYEHCARAHWQVRFRWQPDSVAFWDNRCVQHIAMWDYFPDTRSGWRVTVKGDKPFHR
                     AN"
     gene            1909..2340
                     /locus_tag="VKQ27_02565"
     CDS             1909..2340
                     /locus_tag="VKQ27_02565"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLY87846.1"
                     /translation="MASIKRHASAEWQGSGKDGKGSLTTQSATLKDTPYSFTARFGDG
                     KGTNPEELIAAAHAGCFSMATAFQLSGAGHAPESLHCDATLTMEQEGAGWKIAAVHLV
                     LKARVPGLDNAKFQELAAGAKANCPVSKVLNAAITLDATLE"
     gene            complement(2468..3562)
                     /gene="ychF"
                     /locus_tag="VKQ27_02570"
     CDS             complement(2468..3562)
                     /gene="ychF"
                     /locus_tag="VKQ27_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLY87847.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTATVAAQAANYPFCTIEPNVGR
                     VAVPDPRLAALAAIGKSAKIVPTSLEFVDIAGLVRGASKGEGLGNQFLANIREVDAII
                     HVLRCFEDPDITHVEGPIDPIRDADVVETELMLADLDSLEKRLVAAQKRARGGDKEAI
                     ASVALMEPIVTALQDGKPARTAVPPGQTEAANRLQLLTTKPVLYVCNVEEATAAEGNA
                     FSARVQERAKAEGARTVIVSAAIEAEVAQLPEGDRAEFLEGLGLHDSGLDRVIRAGYD
                     LLGLITYFTVGPKETHAWTIVRGTKAPQAAGAIHGDFERGFIACETIGYDDYVACKGE
                     VGAKEAGKMRVEGKEYVVKDGDVLLFRFNV"
     gene            complement(3705..4283)
                     /gene="pth"
                     /locus_tag="VKQ27_02575"
     CDS             complement(3705..4283)
                     /gene="pth"
                     /locus_tag="VKQ27_02575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLY87848.1"
                     /translation="MLLWVGLGNPEPGMARNRHNIGFMAVDTIAIAHRFSPWRQRFKG
                     LTSEGTIAGEKILALKPLTYMNDSGASVQQAAAFYKLAPEAITAFHDELDLVPGKVRV
                     KRGGGAAGHNGLRSMDRILGTQDYWRVRLGIGHPGTKERVMGHVLGDFMKDDRDWLLP
                     LLDAVADAAPLLAQGKPEDFMTKIALLMKDAR"
     gene            complement(4296..4964)
                     /locus_tag="VKQ27_02580"
     CDS             complement(4296..4964)
                     /locus_tag="VKQ27_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HLY87849.1"
                     /translation="MANAVTIEAEARARAGKGAARATRRGGKVPAVIYGAKLAPTLIA
                     LDPRAVLRELQRPGWQSRQYEINVTGGDTTRALIRDVQFHPVTDAPEHVDFQRLIAGQ
                     QVRVSVPVHFENEGLSPGLKRGGVLNVVRHTVEVYTDPEHIPDQFTANLADKDFNDAV
                     RWEDLEGRGNTRAAIPRNFVVATVAPPTKIEVAAEAAAAPGAAPAAAAAPAGGARPAP
                     AKRG"
     gene            complement(5097..6317)
                     /locus_tag="VKQ27_02585"
     CDS             complement(5097..6317)
                     /locus_tag="VKQ27_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87850.1"
                     /translation="MKAETRLVACVCLAEILGLAGFSLVPALLPQFIATWSLSNIQAG
                     WFAGIMSGGYMIGVLPLVALTDRAPARTIFLISSALSALSSIGIALSNSLLPALLWRA
                     VGGVALAGMYMPGLRALTDGMSGATRARAAAWYTSSFALGSSLSFLLGQAGLRWDWHY
                     AFLVSGALGAVAVLLAWAVLPRRNTASFAGTANAMPSFRRVLGHRDVLALVVGYTATI
                     WGTAGLRQWIVVFLAFCAADQGGSAGQDWSMLTVGALVGILGVPASLCGNELSLRLGL
                     RATAMGVFLVSALVNAAFGFAAALPYSAVVVLAIAAGFIVQGNFSNLTSGLLAIAEPR
                     LIGVTVAVYSCIGFAGGFVGTLLFGITLDFFGGTSRPAAWVLAFGTCAAACLAGSAAS
                     IFMPRYLVRERAVQ"
BASE COUNT         1146 a         2011 c         2424 g         1160 t
ORIGIN      
        1 ggcggccgtg ttcccggcct tcgtcctgct cgccgtgctg atgctgctgg tcgcgcttgc
       61 ctacgtgttc ctcggtgttg agacgcacgg ccgtccgatc gcgcagggcc acgaggagct
      121 ggcgtagcta cggggcgtat atcggcggtg gcgggaggct gccgccgaaa cgcgtgccgc
      181 ggttatggtg gcgcgggggg gtggtctgtg cggctggaag aataaaggaa ccgcaaagca
      241 cgcagaacac gtagaggacg gcgatgcgtt atggagcagg aacggtgcat gattcgacca
      301 aatcttgcca ggctctagtg ccgccggcgg cgtgggggcg tggcgacggt gactatcttg
      361 cagtgaggca agcgggcgag cgcggcggga tcggctgata cgccgcgtag gtcgatgcgc
      421 cgcaaattgg tcagcgcggc gagcggcgtc aggtcggtca ctttggtgtt ggtcagatcg
      481 aggtactcca gcgctgtcag cttcgcgagc gccgtgatgt cgcggatggt ggtgttggag
      541 aggtcgaggc agtgcaggtt cggcaggttc gatagcggtg atgcgtcgat gatcggtgtg
      601 tcaccgagat cgatgcgttc aagcctggtc agtccggtga gcggcgcgat gtcggtgacc
      661 agtgtgccgg tcaggaagca ggcttgaagt tcgcgcatgt gggcgagcgg cgacaggtca
      721 cgcaccgcgg tgaacgagag gtgcaggctc tgcatatgca ccagtggtga caggggcgcg
      781 atgtcgctga tgcgggagcg ggtcaggtgg agctggcgca ggtgtgtcag cgcgctgagc
      841 ggcgccaggt cggagaggta gctacgctcg aggcgcaggt cggtgatgtg cggaatccac
      901 tctggcggca gcgcctgacc cgcgaggatc aggtgcgccg cctcggcggg atcaattcgc
      961 acgatggaaa ggcttgtcgc ccttcacggt gacgcgccag cccgagcgtg tgtcggggaa
     1021 gtagtcccac atcgcgatgt gctgcacgca gcgattgtcc cagaatgcaa ccgaatccgg
     1081 ttgccagcgg aagcgcacct gccagtgggc gcgggcgcaa tgctcgtaga ggaagtcgag
     1141 gatcgccttg ctctcgcctt ccggtacatc cttgatcgag tgggtgaaca ccggattgac
     1201 gaagatcgcc tggcggccgc tgaccggatg cgtgcggatc accggatgct cggcgcgcgg
     1261 gaaggtcttc gggccgtcgc gtccgatcag gttgttggtg cgggtgtagt tcatcgagcc
     1321 gtcatggatc gcggtgagct gcgacaggta ctgcttcatc ggttccgaca gcgcgtcgta
     1381 ggccgcgtac atgctggaga agatcgtgtc accgccaacc ggcggaacga ccttcaggtt
     1441 caggatcgaa cccatcgggg gctcgggatc gcaggagacg tcggagtgcc agcgctcgcc
     1501 gttgatgcgc ttggagttcg cgtcggcgta gatcggcaga atttcggggt ggccttcggg
     1561 gccaggtgtg ttcgggtgga tgtgcaactc gccgaacagg cggccgaacg ccttgtgctg
     1621 gtcgagcgtc agcttctggt cgcggaagaa cagcacctgg tgcgccatca gcgcgtcgtg
     1681 cacttcctgg aactgctgat tgccgagcgg cttgccgaga tcgatgccga atacttcggc
     1741 gccgatgacg gcggtgacgg gcttcacctc gatggtctcg tagggcatgg gactgctcct
     1801 ctgggacgtt tgcttgaact atccgccgca accgcgcggc tggcaaattc gttcaagcgg
     1861 gtgttggcgc agattccgta ggctgggcgc ggtagcagga gaaccgatat ggcgagcatc
     1921 aagcgtcacg cgagtgccga gtggcagggc agcggcaagg acggcaaagg gagcctgacg
     1981 acgcagagcg cgacgctgaa ggacacgccg tattctttca cggcgcggtt cggcgatggg
     2041 aagggtacca acccggagga gctgatcgcc gccgcgcatg ccggctgctt cagcatggcg
     2101 acggcgtttc agctcagcgg ggctgggcat gcgccggaga gcctgcattg cgatgcgacg
     2161 ctgacgatgg agcaggaagg ggcgggctgg aagattgccg cggtgcatct ggtgctgaag
     2221 gcgcgtgtgc cggggctcga caacgcgaag ttccaggaac tggcggcggg cgcgaaggcg
     2281 aactgcccgg tgtcgaaggt gctgaacgcg gcgattacgc tggacgcaac gctggagtag
     2341 tggagggcgt cgcgtggggc gcttcgcccg gcggattggt gcggcggtag gctgatggcc
     2401 ggctcaaggc cggccatgac ggtgggggac agcggggcgg gccgtaacgg gaggcgacag
     2461 gacgcgatta cacgttgaag cggaagagca ggacgtcgcc gtctttcacg acgtactcct
     2521 tgccttctac gcgcatcttg ccggcttcct tggcgccgac ctcgcctttg caggcaacgt
     2581 agtcgtcgta gccgatggtt tcacatgcaa tgaagccgcg ctcgaagtcg ccgtggatgg
     2641 cgccggcggc ctgtggggcc ttggtgccgc ggacgatggt ccaggcgtgg gtttccttgg
     2701 ggccgacggt gaaataggtg atcaggccga gcaggtcgta gccggcgcgg atcacgcgat
     2761 ccaggccgct gtcgtgcagg cccaggcctt cgaggaattc cgcgcggtcg ccttccggta
     2821 gctgggcgac ttcggcttcg atcgcggctg atacgatgac ggtgcgggcg ccttcggcct
     2881 tggcgcgttc ctgcacgcgg gcggagaatg cattgccttc cgccgcggtg gcttcctcga
     2941 cgttgcagac gtagagcacc ggcttggtgg tcaggagttg cagacggttg gcggcttcgg
     3001 tctggccggg cggcactgcg gtgcgcgccg gcttgccgtc ctgcaatgcc gtgacgatcg
     3061 gttccatcag ggcgacggat gcaatcgctt ccttgtcgcc gccgcgggcg cgtttctgcg
     3121 cggcgacgag gcgcttctcc aggctgtcga gatcggccag catcagttcg gtctcgacca
     3181 cgtcggcatc gcggatcgga tcgatggggc cttcgacatg ggtgatgtcg ggatcctcga
     3241 agcagcgcag tacgtggatg atggcgtcca cttcgcggat gttggcgagg aactggttgc
     3301 ccaggccttc gcctttgctc gcgccgcgca ccaggccggc gatatcgacg aattccagag
     3361 acgtgggcac gatctttgcc gatttgccga tcgcggcgag ggcggccaga cgcggatcgg
     3421 gcacggcgac acggccgaca ttcggctcga tggtgcagaa cgggtagttc gctgcctgcg
     3481 cggcaaccgt cgcggtcagc gcgttgaaca gcgtggactt gccgacgttg ggcaggccga
     3541 cgatgccgca gttgaagccc atgggtcagc cctgcgccgc aggcaaggtg attggaacca
     3601 cagatggacc ccgatggaca cagatagggc ctgctgaggg gcgcgagtgc cggtcccccg
     3661 gaaagcatct gtgtccatct gtgtgcatct gtggttccat tttcttatct cgcatctttc
     3721 atcagcaatg cgatcttcgt catgaagtct tccggcttgc cttgtgccag cagcggcgcg
     3781 gcatctgcca cagcatcgag cagcggcaga agccagtcgc gatcatcctt catgaagtcg
     3841 cccaggacgt ggcccatgac gcgctccttg gtgcccggat gaccgatgcc caggcgcacc
     3901 cgccagtaat cctgggtgcc caggatgcgg tccatgctgc gcagcccgtt atgcccggcc
     3961 gcaccgccac cacgcttcac ccggaccttg cctggcacga ggtcgagttc gtcgtggaag
     4021 gcggtgatcg cctcaggcgc cagtttgtag aaggcggccg cctgctgcac cgaggcgcct
     4081 gagtcgttca tataggtcag cggcttgagc gcgaggatct tctcgcccgc gatcgtcccc
     4141 tcgctggtca ggcccttgaa gcgctggcgc catgggctga agcggtgggc aatggcgatc
     4201 gtatcgaccg ccatgaagcc gatattgtgc cgattccggg ccatccccgg ctccggattg
     4261 ccgaggccga cccagagcag catggcgcgc ctcgatcagc cccgcttggc tggggccggg
     4321 cgcgcaccac ccgcgggagc cgccgctgct gccggagcag cgccaggtgc cgcggcagct
     4381 tctgctgcaa cctcgatctt ggtgggcggc gccaccgtgg cgacgacgaa gttgcggggg
     4441 atcgccgcgc gggtattccc gcgaccctca agatcttccc agcgaacggc gtcgttgaag
     4501 tccttgtcgg ccaggttggc ggtgaactgg tcggggatgt gctcggggtc ggtgtagacc
     4561 tcgaccgtgt ggcgaaccac gttcaacacg ccgccgcgct tcaggccggg gctgaggcct
     4621 tcgttctcga agtgcaccgg cacagagacg cgcacctgct ggccggcgat caggcgctgg
     4681 aaatcgacgt gctcgggggc gtcggtcacc gggtggaact gcacgtcgcg gatcagcgcg
     4741 cgcgtcgtgt cgccgccggt aacgttgatt tcgtactggc gcgactgcca gccggggcgt
     4801 tgcagctcgc gcagcaccgc gcgcgggtcg agcgcgataa gggtgggcgc cagcttggcg
     4861 ccgtagatga cagcggggac cttgccccca cgccgagtcg cccgcgccgc ccccttaccg
     4921 gctcgcgcgc gtgcctcggc ctcgatcgtc acagcattgg ccatggtacg ctcctgcaaa
     4981 tggcaacgcc ggcctccagg ggtgccggcg tggcggggga tttagcgggg atggcgggcg
     5041 ggggcaaggc gcgagagcct gcacccacgg gcatctggca ggcggccggg ttgcggctac
     5101 tgaaccgcgc gttcccttac caggtaacgc ggcatgaata tgctggcggc gctgccggca
     5161 aggcaggcgg cagcgcaggt gccgaatgcc agtacccagg cggcgggccg cgaagttcca
     5221 ccgaagaaat cgagggtgat gccaaacagc aatgtgccga caaagccgcc ggcgaagccg
     5281 atgcaggagt atacggctac cgtcacgccg atcagccgcg gctcggcaat cgcgagcaag
     5341 cccgaggtga ggttggagaa attgccttgc acgatgaaac cggcagcgat cgcgaggacc
     5401 acgacggcgc tatatggcag cgctgcggcg aagccgaacg cggcattcac caatgccgat
     5461 accaggaaca cgcccatggc cgtggcgcgc aggccaaggc gcaacgacaa ttcattgccg
     5521 cacagacttg ctggcactcc aaggatcccc accagcgcac cgacggtcag catgctccaa
     5581 tcctgcccgg cgcttccgcc ctggtccgcg gcacagaatg cgagaaagac cacgatccat
     5641 tgccggagcc cggcggtgcc ccagatcgtt gccgtgtagc cgacgaccaa tgccagcacg
     5701 tcgcgatgac cgaggacgcg gcggaagctc ggcattgcgt tggcagtgcc ggcgaagctt
     5761 gcggtgtttc tgcggggcag cacggcccag gcaagcagca ccgcaacggc gccaagtgcg
     5821 ccactcacca ggaacgcata gtgccaatcc catcgaaggc ctgcctggcc gagcaggaaa
     5881 gacagcgacg aaccgagggc aaacgaactg gtgtaccagg cagcggcacg ggcgcgggtc
     5941 gcacccgaca tgccgtcggt gagtgcgcgc aagccgggca tgtacatgcc ggcgagcgct
     6001 acgccgccca ctgcgcgcca gagtagtgcc ggcaacagac tgttgctcag ggcaatgccg
     6061 atcgacgaca gcgcgctcaa tgcgctgctg atgaggaaga tcgtgcgcgc cggagctcga
     6121 tcggtaagcg cgaccagcgg cagcacgcct atcatgtagc cgccggacat gatgccggcg
     6181 aaccagccag cctggatgtt gctcaatgac caggtcgcga tgaactgcgg cagcagggct
     6241 gggaccagcg agaagccggc caggccaagg atttccgcca ggcagacaca ggcgacgagg
     6301 cgggtttcag ccttcatcta ccgcgtgtat cggacggacg tcgatcatcg ttgcgtccct
     6361 tcggatggcg aggcggtcgt ggcggcttgg gctgcatcga gcgcattata ccggagccag
     6421 atcgaacggt tcgacgctgc cgctgacctc ttctgcaagt tccaatggcc gcgctccaca
     6481 ccatgcgggc atgcgttacg gtggcggata ttccttgtcg gcgccagtcg gggcctcact
     6541 ccgccagggc ttggcggagt gctcggtggc tgggtgattg cggggtcgaa caggtgttgg
     6601 cggtccggca agctgtatgc cgatgagccg ctatcagagg gcaaccggga gcgcacgcga
     6661 gccatcatcc gattgcgcaa aagcgaacgc atcgtgcgtg ttccggtggc cacgaaccga
     6721 ccaaccctca atcccagcgt t
//
LOCUS       DATGEM010000078         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_210179, whole genome shotgun sequence.
ACCESSION   DATGEM010000078 DATGEM010000000
VERSION     DATGEM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210179"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VKQ27_02590"
     CDS             <1..798
                     /locus_tag="VKQ27_02590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HLY87851.1"
                     /translation="PLEEAYAYADVEGVTVRTALDGIGYRGDQPATPFPADAYFELHI
                     EQGPILEREVTDIGIVTGAQAMVWYDAVAIGQDSHAGTTPPSARKDALVCAARIIDLV
                     DRMMRARGEDGRGTVGQLHVLPNSRNVIPGEVRFSVEFRHPSDAEVDRLDAQFPREAS
                     FIARDSGVKLELTQLFKIPAQPFDPACVDLVRNGAAKLGYTAREIVSGAGHDAVYVAR
                     HVPTAMIFTPCKDGLSHNEAESIEPHEAEAGAQVLFEAVLARANRTL"
     gene            918..2396
                     /gene="gabD"
                     /locus_tag="VKQ27_02595"
     CDS             918..2396
                     /gene="gabD"
                     /locus_tag="VKQ27_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLY87852.1"
                     /translation="MLNPTSPLPLKDPTLFRQANCIEGKWVQADSGKTTIVKNPATGE
                     AIGEIPALGAAETRRAIEAANRAQPAWRAMLAKERAAILRKLNDLMMENADDLAVIMT
                     AEQGKPLAESKGEIAYAASFIEWFAEEGKRIYGDTIPQNAKGRRIIVLKEPIGVFAAI
                     TPWNFPAAMITRKAGPGWAAGCTGVIRPASQTPFSALALAVLAERAGMPPGVCNVITG
                     PSGETGAELTSNPLVRKLTFTGSTEVGAKLLAQCAPTIKKTSMELGGNAPFIVFDDAD
                     LDAAVVGAMASKYRNTGQTCVCANRFLVQDGVYDAFSAKLKAAVEAMKVGNGMEPGVT
                     QGPLINADAVKKVEEHIADAVKRGAKVVTGGKRHTLGGNFFQPTILANVPNDAMIFRE
                     ETFGPVAPLFRFKTEEEAIKLANDTEFGLASYFYARDVGRIFRVAEALEYGIIGINEG
                     IISTEVAPFGGMKSSGLGREGSKYGIEDYLEIKYLALGGIGT"
BASE COUNT          514 a          919 c          792 g          414 t
ORIGIN      
        1 ccgctggaag aggcctacgc gtacgccgac gtggaaggtg tcacggtccg caccgcgctc
       61 gacggcatcg gctatcgagg cgaccaacct gccacaccct tcccggccga tgcgtatttc
      121 gagctgcata tcgaacaagg cccgatcctg gaacgcgaag tcactgacat tggcatcgtg
      181 accggagccc aggcgatggt ctggtatgat gcagtcgcga ttggccagga ttcgcacgcc
      241 ggcaccacgc caccctccgc ccgcaaggac gcgctggtct gcgccgcccg catcatcgac
      301 ctcgtggacc gtatgatgcg cgctcgcggc gaggacggtc gcggcaccgt cggccaactc
      361 cacgtcctcc cgaacagccg caacgtcatc cccggcgagg tccggttctc tgtcgaattc
      421 cgccacccat ccgatgcgga agtcgatcgg ctcgacgcgc agttcccccg cgaggccagc
      481 ttcatcgccc gcgactccgg cgtgaagctg gagctgaccc agttgttcaa gatccctgcg
      541 cagccgttcg atcccgcctg cgtggacctg gtccgcaacg gcgcggcgaa gctcggctac
      601 accgcccgcg agatcgtctc cggtgccggc cacgacgcag tatatgtcgc gcgccatgtc
      661 cccaccgcga tgatcttcac cccctgcaag gatggcctct cgcataacga ggcggaaagc
      721 atcgaacccc acgaagccga agcgggcgcg caggtgctgt tcgaagctgt tctggccagg
      781 gccaaccgca cgctgtagcc gcctgccgca cgttctcacc caaggccacg tgagacgctg
      841 ccaagattgc ctgccccctg acactgtccg atccgtgtcc tatgttgcct cgacatgcga
      901 gccaggagga caacgccatg ctgaacccga cctcccccct ccccctgaag gaccccacgc
      961 tgttccgtca ggccaactgc atcgaaggca agtgggtgca ggcagacagc ggcaaaacga
     1021 ccatcgtgaa gaaccccgcg accggtgagg cgatcggcga aatccccgcg ctgggcgccg
     1081 ctgaaacccg ccgcgcaatc gaggcggcaa accgcgcgca gcctgcctgg cgcgccatgc
     1141 tggcgaagga gcgtgcggcg atcctgcgca agctcaacga tctcatgatg gaaaatgccg
     1201 atgacctcgc cgtgatcatg acggccgagc agggcaagcc gctcgccgag agcaagggcg
     1261 aaatcgcgta tgccgccagt ttcatcgagt ggttcgccga ggaaggcaaa cgtatctacg
     1321 gcgacaccat tccgcagaac gccaagggcc gccgcatcat cgtgctgaag gagcccatcg
     1381 gcgtcttcgc cgcgatcacg ccgtggaact tccccgccgc gatgatcacg cgcaaggccg
     1441 gccccggctg ggccgccggt tgcacgggtg tcatccgtcc agccagccag acgccattct
     1501 ccgccctcgc cctcgcggtg ctggccgagc gcgccggcat gccccccggc gtgtgcaatg
     1561 tcatcaccgg tccatccggt gagaccggtg ctgaactcac ctccaacccg ctggtgcgca
     1621 agctgacgtt caccggcagc accgaagtcg gcgccaaact gctcgcgcaa tgcgcgccca
     1681 cgatcaagaa gaccagcatg gagctcggcg gcaacgcgcc gttcatcgtg ttcgatgatg
     1741 ccgatctcga tgcggccgtc gttggtgcca tggcgagcaa gtatcgcaac accggccaga
     1801 cctgcgtctg cgccaaccgt ttcctggtcc aggacggtgt gtatgacgcg ttctcagcca
     1861 agctgaaggc agcggtcgag gcgatgaagg tcggcaacgg catggaaccg ggcgttaccc
     1921 aaggtccgct gatcaacgcc gacgcggtga agaaggtgga agaacacatc gccgacgcgg
     1981 tgaagcgcgg cgccaaggtc gtgaccggcg gcaagcgcca cactctgggc ggcaatttct
     2041 tccagccgac aattctcgcc aacgtgccga acgatgcaat gatcttccgc gaggaaacct
     2101 tcggccctgt cgcgccgctg ttccgcttca agaccgagga agaagcaatc aagctcgcca
     2161 acgacacgga gttcggcctc gcatcgtatt tctatgcgcg tgatgtcggt cgcatcttcc
     2221 gcgtcgccga agccctggaa tacggcatca tcggcatcaa cgagggcatc atctccaccg
     2281 aggtcgcgcc gttcggcggc atgaagtcat ccggcctcgg ccgcgaaggc tcgaaatacg
     2341 gtatcgagga ctacctggag atcaagtacc tcgcgctcgg cggcatcgga acgtagcaga
     2401 ggcggcgtcg cgaactgaca ttgcgagacg cgaagcgacg aagcaattct cggtcgtgat
     2461 gcgccacgtt cctgagattg ctttgtggct acgcacctcg cagcgacgtg gtgctcgcca
     2521 gcaacacgca ccgcgccgac cccgaccccg ctggccctga agtcataatg cttggcaacg
     2581 caccgcacac cggaggaacc cgtcgcaatg cccgccacgc ccacctacga agtccttgc
//
LOCUS       DATGEM010000079         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_886658, whole genome shotgun sequence.
ACCESSION   DATGEM010000079 DATGEM010000000
VERSION     DATGEM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886658"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1983)
                     /locus_tag="VKQ27_02600"
     CDS             complement(22..1983)
                     /locus_tag="VKQ27_02600"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87853.1"
                     /translation="MANAGSLGRRLDSLIALIDWTRQGAQDADGRPDPSRLIKAIEVL
                     EALPAVRVGLQDTFAEILSETEGVNLFGESGIPGDRGFIAELTDRVMGRILPEPSDDH
                     DLARLVSRLYSSRAKAESFDLLAPELFDRIVRALAPDDRPQIWAPLKTAFADGFRLLA
                     IRVQAQGLSTKLRARSHPASVAQSPFHLVALASDALMDAWRAGEDVAALAGNWRELCA
                     ACRTETAEVSRRLESEGVSVDIVYGLEVLERCLSRMQAMLAVTASPPGTPRNEAIHHL
                     LAGLIVAAHDDRSIRHLIRGNLQMLQRKIVDRSGKTGEHYVAHSRREYRFIWLAAAGG
                     GLLTVLTAAIKLKVTHTGLPLFVEGLCAGFNYAVSFMLLHHLHLILATKQPAMTAATL
                     ARLLRSRDRTERLDRMVEFTVRICRSQFAAAIANVSVVFVGAFVFNFLWRLALGRNYL
                     GATEAQHVFELLSPVNSGTVFYAALTGVILWMAAMIGGWMDNWAVYHRLPQAIADHRF
                     GERFGRPRMARAAGIVARNMSGWATNISLGFMLGLTPVIGQFLGLPIDVRHVTLSSGM
                     LAFAGAGLRGWFTTGWFFWALAGVATMFVLNLGVSFFLSLYTASRAYNLARKELAVLG
                     ARLLRRFVQRPLDFILPRKLEEEGGKKQY"
     gene            1906..2079
                     /locus_tag="VKQ27_02605"
     CDS             1906..2079
                     /locus_tag="VKQ27_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87854.1"
                     /translation="MRTLPRPVDQRDERIEPAAETARIGDGLETFGEFERIVIRSDAA
                     VEEGDNLFAKRRG"
BASE COUNT          425 a          681 c          690 g          364 t
ORIGIN      
        1 tcatcgtcca tagaggcgtg ctcagtactg ttttttgcct ccctcttcct ccagtttccg
       61 cgggaggatg aaatcgagcg gtcgctggac gaagcgccgc agcaacctcg ccccgagtac
      121 cgccagttcc ttgcgcgcca ggttgtatgc gcgcgatgcc gtgtacaggg acaagaagaa
      181 actcacgccc aggttcagga cgaacatcgt cgccacgccc gcaagcgccc agaaaaacca
      241 gccggtggtg aaccatccgc gcagcccggc tcccgcaaac gccagcattc ccgaagacag
      301 ggtgacgtga cggacatcga tcggcaaccc gagaaactgg ccgatcacgg gcgtcagacc
      361 gagcatgaat ccgagcgaga tgttggtcgc ccagcccgac atgttgcgtg ccacgatgcc
      421 ggccgcgcgc gccatgcgcg ggcgaccgaa acgttcgccg aatcggtgat cggcaatggc
      481 ctgcggcagc cggtgataga cggcccagtt gtccatccag ccgccaatca tggcggccat
      541 ccacaggatc actcccgtca gcgcggcata gaacaccgtg ccgctgttga ccgggctaag
      601 tagctcgaac acgtgctgcg cctccgttgc gccgagatag ttgcggccga gggcaaggcg
      661 ccagagaaaa ttaaacacaa atgcgccgac gaataccacc gatacgttgg ctatcgcagc
      721 ggcaaactgg gaccggcaga tgcgcaccgt gaactcgacc atcctgtcga ggcgctcggt
      781 gcgatcccgg ctgcgcagca acctggcaag cgtggccgcg gtcatggcag gttgcttggt
      841 ggcaaggatc aggtgcaagt ggtgcagcag catgaagctg acggcgtaat tgaagccggc
      901 gcacaagccc tcaacgaaca gcggcaatcc ggtgtgcgtg accttcagct tgatggccgc
      961 cgtcagcaca gtgagcaaac cgccgcccgc ggcggcgagc cagatgaaac gatactcgcg
     1021 gcggctgtgc gcgacatagt gctcgccggt ttttccggac cggtcgacga tcttgcgctg
     1081 gagcatctgc agattgccgc ggatcagatg gcggatgctg cgatcgtcat gcgcggcgac
     1141 tatgagcccc gccagcaggt ggtgtatggc ttcgtttctc ggtgttcccg gaggactggc
     1201 cgtgacggcg agcatggcct gcatgcgcga caggcagcgc tcgagcactt ccagtccgta
     1261 gacgatgtcg acgctgacac cttcgctttc gaggcggcgc gacacttcgg cggtttcggt
     1321 gcggcaggcg gcgcagagtt cccgccagtt ccctgccagt gcagcaacgt cttcgcccgc
     1381 acgccacgcg tccatcagtg cgtcgctcgc cagcgcgaca aggtgaaaag gcgattgcgc
     1441 cacggatgcg ggatggctgc gggcgcgcag cttcgtcgac agaccctgcg cctgtacccg
     1501 gatcgccaat aatctgaagc cgtccgcaaa ggctgtcttc agcggcgccc agatctgcgg
     1561 ccggtcgtcg ggcgcgaggg ctctgacgat cctgtcgaac aattccggcg ccagcagatc
     1621 gaagctttcg gccttggcgc ggctcgaata cagccgcgac accagccgcg cgagatcgtg
     1681 atcgtcgctc ggttcgggaa gaatgcggcc catgacacgg tcggtcagtt cggcaatgaa
     1741 tccacggtcg ccgggaatgc ccgattcgcc gaacaggttc acgccttccg tctcggaaag
     1801 aatttcggcg aacgtatcct gcaagccgac gcgcacggcg ggcagcgcct cgagcacctc
     1861 tatcgccttg atgagccgcg aaggatccgg ccgtccatcc gcgtcttgcg caccctgccg
     1921 cgtccagtcg atcagcgcga tgagcgaatc gagccggcgg ccgagactgc ccgcattggc
     1981 gatggcctcg aaacattcgg cgagttcgaa cgcatcgtga tcagatccga tgccgcggtc
     2041 gaggaaggcg acaacctgtt cgccaagcgt cgggggtaag agatgtatgt tcatcagcgg
     2101 cgggagtttg tcacggtaag aggggaagta tcacacgaag ttcgcgcgct ctgcggacat
//
LOCUS       DATGEM010000080         4900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1686988, whole genome shotgun sequence.
ACCESSION   DATGEM010000080 DATGEM010000000
VERSION     DATGEM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686988"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VKQ27_02610"
     CDS             complement(<1..1733)
                     /locus_tag="VKQ27_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY87855.1"
                     /translation="MTVLSVIGDTPRRREDVRFTTGHGSYLDDLQFDRLAHAVVLRSP
                     RAHALIRRVDTAAARAMAGVLAVLTGEDARADGLQPMQPTADTNVQTGEKLTFGPQPL
                     LAGRKVRHVGEPVAVIVAETRALAMDAAEAVIVEYETLPAVTGVATVPLIADEVPGNV
                     CMDWSWGDAAAVDAAFASAAHVVALDVGNHRIVTNPMEPRGAIGAFADGRYTLHVSSQ
                     NIHGNRDATARHLGVPPSDVRFVAPDVGGGFGAKNFTYAEHALILWAAKRVRRPVKWI
                     ATRSEGFVSDHQARDHQAQASLALDAEGGFLALRVHSVANIGAYMAGGAGAVQTNQYV
                     HLQGSIYAVPSIALRVVTVLSNTTPIGVTRGPGFAESANIMERLIDAAARQCGFDRME
                     LRRRNMAQAPMVNALGFSVDSGDFVRTLDRALQAADYQGFAARRTESEARGMLRGLGL
                     AYHIKGTGGSPHENVDIRFESDGTVSLITGTQTIGQGHETTFPQILADRLGIPNELIR
                     LRQGDTDLIPMGGGHGSSRATYMGGTAIWRASETIVEKGRVVAAEMLEAAEADIAFAD
                     GHFAVIGTNRSV"
     gene            1911..3236
                     /locus_tag="VKQ27_02615"
     CDS             1911..3236
                     /locus_tag="VKQ27_02615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87856.1"
                     /translation="MTAKRQLHLGAFMRPTTIHTASWRYPGAYPDANFNLSHLKRFIQ
                     TLERGKFDAFFMADHLAVLNMPMNALKRSHTVTSFEPFTLLSALAMATDHIGLIATGS
                     TTFDAPFHVARRFASLDHISGGRAAWNIVTTSNPDAALNFGLEEALDHDERYRRAREF
                     YDVVTGLWDSWAEDAFTRNVETGEFFDPARMHVLDHKGEFYNVRGPLHIARPIQGWPV
                     IVQAGGSDVGRQLAAETAEAVFAGAGDITGGRALYADIKGRMAKIGRDPDHLKILPGA
                     FVVIGDSLDEARDKRAKLDSLVHYDSAIASLSIALGTDASKLDPDAPLPDIPESNASK
                     SGRERAINLGRRDNLTVRQLAQRMGGYSGPALLGTPTMIADQMEEWLTTDACDGFNIM
                     FPFLPAGLDDFVERVVPELQRRGIFRKEYTGKTLRENLGLPRPENRFFA"
     gene            3387..4640
                     /locus_tag="VKQ27_02620"
     CDS             3387..4640
                     /locus_tag="VKQ27_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLY87857.1"
                     /translation="MTRRAIVVVLDGLRRDMVGPSTTPNLHAFAAHATSFAAHRSVFP
                     SATRVATSCFATGCLPAAHGLQGNAVALMEHGQLVHHDVGHPDFMDHWRRVQGRTFAV
                     PTLAERLARTGRNAMVFNNVSPGAARAHDPDGHGWVFNRAWSHAPGLRPLTGADAVPG
                     VTLDTEGDARLTDYFLQRARHADLAVLWLGDPDHSQHEHPLGSPEAMAAITAADACFG
                     RIRATVTPDTQLIACSDHGHQTVGEVVDINAEFAAAGLKLETDDPSLLAASNGTSALI
                     YLHPDRAGDAHAVLDFLGAQPWAGVILAGDTLRAAGHAPEGGLLCAVSMRSEDHPNEY
                     GIPGTSAAARPVVGKPDRLGCGQHGGLGAYEQMPFLQIEGAGFAPSATRNDPSSLIDL
                     APTILAHLGLPANGCEGRALQTLEA"
BASE COUNT          864 a         1951 c         1307 g          778 t
ORIGIN      
        1 actgaccggt tcgtcccaat cacagcaaag tgcccatcgg cgaacgcgat atccgcctcc
       61 gccgcctcca gcatctccgc cgcgacgacc cgccccttct ccacaatggt ttccgacgcc
      121 cgccagatcg ccgtcccccc catatatgtc gcgcgcgaac taccatgccc gccgcccatc
      181 ggaatcagat ccgtatcccc ctgccgcagc cggatcagct cgttcggaat ccccagccgg
      241 tccgccagga tctgcgggaa cgtcgtctcg tgcccctgtc cgatcgtctg ggtcccagtg
      301 atcagcgaca ccgtcccatc cgactcgaac cggatatcaa cgttctcgtg cggtgacccg
      361 ccggtcccct tgatatgata ggcaagccca agcccgcgca gcattccccg cgcctcactc
      421 tccgtccgcc gcgctgcaaa cccctggtaa tccgccgcct gcaacgcgcg atcgagcgtg
      481 cggacgaaat ccccactatc gaccgagaat ccgagcgcat tcaccatcgg cgcctgcgcc
      541 atattccgcc gccgcagctc catccgatcg aacccgcact gccgagccgc ggcatcgatc
      601 agtcgctcca tgatattcgc cgactcggca aatcccggtc cccgcgtaac cccgatcggt
      661 gtcgtattgc tcagcactgt aacaacccgc agcgcgatcg acggcacggc gtagatactg
      721 ccctgcaggt gcacatactg gttggtctgc accgccccgg cgccccctgc catatacgcc
      781 ccgatattcg ctacgctgtg cacccgcagc gcgaggaatc cgccctccgc atccagcgcc
      841 agcgaagcct gcgcctgatg gtcgcgggcc tgatgatcgg acacgaaccc ctcgctgcgc
      901 gtcgcgatcc atttcaccgg ccgcctaacg cgcttcgccg cccacaggat cagcgcatgc
      961 tcggcataag tgaaattctt cgcgccaaat ccgccgccca catcaggcgc cacgaaccgc
     1021 acatcgctcg gcggcacccc cagatgccgc gccgtcgcat cgcgattgcc atggatattc
     1081 tggctcgaca catgcagcgt ataccgcccg tcagcgaacg cgccgatcgc ccctcgaggc
     1141 tccatcgggt tcgtaacgat ccgatggttg cccacatcca aagccaccac gtgcgccgcc
     1201 gaagcgaacg ccgcatccac cgccgcggca tcgccccagc tccagtccat gcagacattg
     1261 cccggcacct cgtcagcaat cagcggcacc gtcgcgacac cggtcaccgc cggcagagtc
     1321 tcgtattcaa caatgaccgc ctccgctgca tccatcgcca gcgcacgcgt ctccgccaca
     1381 atcaccgcga ctggctcacc cacatgccgc accttgcggc cagccaacag cggctgcggc
     1441 ccaaacgtca gtttctctcc tgtctgcaca tttgtatctg cggtcggctg catcggctgc
     1501 aacccatccg cccgtgcatc ctcgcctgtc agcaccgcca gcacaccagc catcgcccgc
     1561 gccgctgccg tatccacacg cctgatcagc gcatgcgcac gcggcgaccg cagcaccacg
     1621 gcatgcgcca ggcgatcaaa ctggagatca tccagatagc tgccatggcc agtggtgaac
     1681 cgcacatcct cccgccgccg cggagtgtcg ccaatcaccg aaagaaccgt catcaccgcc
     1741 tcgcttccgc catcaaacac agtgtcattg cgagcgcagc gaagcaatct cgggtggaat
     1801 tgcgtcgcct cgcttgccat gacctttgct catccgacgg ctgatatatg atccgttgtg
     1861 gcgccgcgtc gggccgacca tcctgagcgc gggcaaaggg accagacact atgaccgcca
     1921 aacgccagct gcacctcggc gccttcatgc gccccaccac catccacacg gcgtcctggc
     1981 gctatcccgg cgcctacccg gacgccaact tcaacctgtc tcacctgaaa cgattcatcc
     2041 agaccctcga gcgcgggaag ttcgacgcat tcttcatggc cgaccacctg gccgtgctga
     2101 acatgccgat gaacgcgctc aagcgcagcc acacggtaac gtcgttcgag cccttcaccc
     2161 tgctgtccgc gctcgccatg gcgaccgacc acattggcct aatcgccact ggctcgacca
     2221 cgttcgacgc gcccttccac gtcgcccgcc gcttcgcctc gctcgaccac atcagcggcg
     2281 gccgcgctgc ctggaacatc gtcaccacct ccaaccccga cgccgcgcta aacttcggcc
     2341 tcgaggaagc cctcgaccac gacgaacgct accgacgtgc ccgcgagttc tacgacgtag
     2401 tcactggcct ctgggacagc tgggccgagg acgccttcac ccgcaacgtc gaaacgggcg
     2461 aattcttcga cccagcccgc atgcacgtcc tcgaccacaa gggcgaattc tacaacgtcc
     2521 gcggccctct ccacatcgcg cgccccatcc agggctggcc ggtgatcgtg caagccggag
     2581 gctccgatgt cggccgccaa ctcgccgccg aaaccgccga ggccgtgttc gccggcgcag
     2641 gcgacataac gggcggccgc gctctctacg ccgacatcaa aggtcgcatg gcaaagatcg
     2701 gccgcgaccc ggaccacctg aagatcctcc caggcgcctt cgtcgtcatc ggcgactccc
     2761 tcgacgaagc ccgcgacaaa cgtgcgaaac tggacagcct cgtgcactac gacagcgcca
     2821 tagcttcgct ctcgatcgcg ctcggcaccg acgcctcgaa attggacccc gacgcgccgc
     2881 ttcccgacat ccccgaatcc aacgccagca agagcggccg cgaacgcgcc atcaatcttg
     2941 gcagacgcga caacctcaca gtgcgccagc tcgcccagcg catgggcggc tactccggcc
     3001 cggccctcct cggcacgccc acgatgattg ccgatcagat ggaggagtgg ctcaccaccg
     3061 acgcctgcga cggcttcaac atcatgttcc cattcctgcc cgccggcctc gacgatttcg
     3121 ttgaacgcgt ggtccccgaa cttcagcgcc gcggcatctt ccgcaaggag tacaccggca
     3181 agaccctgcg cgagaatctt ggcctgccgc gcccggagaa ccgcttcttc gcgtgactat
     3241 ttcggcgccg acgacgccta ccgccaggac gcttcctctc cctcgtcatg gccggccccc
     3301 gagccggcca tcagccccga acggcacagt ctcgcgacgt catccccgca catcgtataa
     3361 cgctccaccc ctcaatggag ccgccaatga cccgccgcgc tatcgtcgtc gtcctcgacg
     3421 gcctccgccg ggacatggtc ggccccagca ccaccccaaa cctccatgcc ttcgccgccc
     3481 acgccaccag ttttgccgcc caccgctccg ttttcccctc cgccacccgc gtcgctacct
     3541 cctgcttcgc gaccggctgc ctgccagccg cccacggcct ccagggcaac gccgtggcac
     3601 tcatggagca cggccagctc gtccatcacg acgtcggcca tccggacttc atggaccact
     3661 ggcgtcgcgt ccagggccgc accttcgctg tccccacact ggccgagcgc ctcgcccgca
     3721 ctggccgcaa cgccatggtg ttcaacaacg tctccccggg agccgcccgc gcccacgacc
     3781 cggatggcca tggctgggtc ttcaaccgcg cctggtcgca cgccccgggc ctccgcccgc
     3841 tcaccggcgc cgacgccgtg cccggcgtca ccctggacac cgaaggcgac gcccgcctga
     3901 ccgactattt cctgcaacgc gcccggcatg ccgacctcgc cgtcctctgg ctgggcgacc
     3961 cggaccatag ccagcatgaa catccgctcg gctcacccga ggccatggcc gcgatcacgg
     4021 cagcggacgc atgcttcggc cgcatccgag ccactgtcac gccggacacg cagctgatcg
     4081 cctgttccga ccacggtcac cagaccgtcg gcgaagtggt ggatatcaac gccgaattcg
     4141 ccgccgccgg cctcaagctg gaaaccgacg acccgagcct gctcgctgcc tccaacggca
     4201 cgtcagccct gatctatctg caccctgatc gcgcaggcga tgcccatgcc gtgctggact
     4261 tcctgggggc ccagccctgg gccggcgtca tcctcgccgg ggacacgctg cgtgcggccg
     4321 gccacgcgcc ggagggcggc ctgctctgcg ccgtctccat gcgcagcgag gaccacccga
     4381 acgagtatgg catcccaggc accagcgccg ccgcgcgccc ggtcgtgggc aagcccgacc
     4441 ggctcggttg cggccagcac ggtggccttg gcgcctatga gcagatgcca ttcctgcaga
     4501 tcgagggcgc cggtttcgcc ccaagtgcca cacggaacga ccccagctcc ttgatcgacc
     4561 tggcaccaac gatcctcgcg catctgggtc ttccggcgaa tggctgcgag ggacgagcgt
     4621 tgcagaccct cgaggcctga cgacattccc aaaggcagcc ggcgaaagcg accgctcggc
     4681 ccgacacgtt gcctccgttg cccggcaact tctgcccgtt gccaatgcgc tttcaccggg
     4741 ccgctgcgat ggcgtccgcc ccccggatgc ggacattgtc acgtacagat cccgtgcata
     4801 gttgtgacct gcacggacga tcaatcgttc cagatagcgc ctcgtagtgg tgccggattg
     4861 tagccggtcc ggcttaccct cgctggtgct ccgggattag
//
LOCUS       DATGEM010000081         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_88587, whole genome shotgun sequence.
ACCESSION   DATGEM010000081 DATGEM010000000
VERSION     DATGEM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88587"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..505
                     /locus_tag="VKQ27_02625"
                     /pseudo
     CDS             342..505
                     /locus_tag="VKQ27_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            607..879
                     /locus_tag="VKQ27_02630"
     CDS             607..879
                     /locus_tag="VKQ27_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HLY87858.1"
                     /translation="MYWDEGAHATPHFHAHHAGRRASVDLEGRVLAGDIDSAALGHVQ
                     EWARIHREELLSNWDRARRSEPILPIEPLEYDLRLWAIFLQLLASR"
     gene            843..1094
                     /locus_tag="VKQ27_02635"
     CDS             843..1094
                     /locus_tag="VKQ27_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HLY87859.1"
                     /translation="MGYLPAVVGVAVIGDHKLRLLFDDGTAGDVDLSRLEWRGVFEPL
                     HDPKYFAQVKFDPDAATVVWPNGADLAPETLYAEAQKTR"
     gene            complement(1185..2504)
                     /locus_tag="VKQ27_02640"
     CDS             complement(1185..2504)
                     /locus_tag="VKQ27_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY87860.1"
                     /translation="MLDLLVIGGGMAGLSAAAWSAERGLHVTVVEKSDQLGGSASMAG
                     YVWTAASADALMSEVPGVDADMGRALVSEIGEGVEWIQSLGVNCHKPITMLRFGRGVP
                     VDMPGYIRACDGVIQHSPHGKLLVSAQTERLLLEEGAVVGAEVRPSDGSVQPLRARFT
                     LLATGGYQNDRALSAQLIHPNASAMPRRTNPHSSGDGLRLGQAAGGQFGKGGAGFYGH
                     LIPFPLDLPDPSMFKILTMFFSEHSLLLNKSGRRFVDETVGDHLSAQAVLEQQGGRAL
                     LVADELVRREWIVQPYVDGIETGDKLGEAARRGGHYASAADLESVGEVAGGWGYDRRA
                     VVDSIRRFNSGGLSLPRRFDARPLVEPPYHLIEVWPAITFTYGGLLTDSHGRVLDENG
                     VAIPGLLAAGADAGGLYVRAYAGGLAPALVFGLRAAKMAANRAFASA"
     gene            complement(2504..3316)
                     /locus_tag="VKQ27_02645"
     CDS             complement(2504..3316)
                     /locus_tag="VKQ27_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87861.1"
                     /translation="MSDYRHLRVEVEGSVAHVVLARPPVNAVNQELYEEIHAAFSSVA
                     DLSGPIKAVILSGDGKHFCAGNDLVEFGTLDPANAPSRMLTVRRAFWAIYDCPVPVLA
                     AVHGAALGTGLALAASCDFIVAARGARFGLPEVGVGVMGGASHVRRLVPEPVARWMFL
                     SGEPAPLDTLVALGAVLEVVEPERLLGVARQKAALLTRHSAVALRIGKRALNETEGAD
                     LKPGYEFEQGRTGDLSGYEDSVEARRAALEQRKPRYQDSQKEADLAARRPFR"
     gene            complement(3313..3741)
                     /locus_tag="VKQ27_02650"
     CDS             complement(3313..3741)
                     /locus_tag="VKQ27_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY87862.1"
                     /translation="MLAADEPVRNVVLRYFHALDRRDYDMVSDCFDENVTATYGGEAM
                     PSGAAALTDELRKRMDRLGVTMHFAGNLLVSIEEDGATAETYAIAFVSDAGAQGAARM
                     RVRGLRYHDRLVLTAAGWKISERVHSVRWSFETDGQVFPN"
     gene            3781..4152
                     /locus_tag="VKQ27_02655"
     CDS             3781..4152
                     /locus_tag="VKQ27_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87863.1"
                     /translation="MLVVGIDVDPEHEEELNRWYDSEHIPQRLGIPGFVRARRLVRAA
                     APHTSGNLKGTPDMPRYLALYEVTDPKVFETVAYRKLVDNPTPWTIRMRDTFELRIRG
                     IYVETLSMSADDPAVEPFRVR"
     gene            complement(4145..>4680)
                     /locus_tag="VKQ27_02660"
     CDS             complement(4145..>4680)
                     /locus_tag="VKQ27_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87864.1"
                     /translation="QKPRPPLWIGAHSAPGIRRAARIGDAWPISPRLPHAEIEVRLRT
                     FFEARREAGLPMGRQPLRREVTIGRDRDDALRRFRSMAQDRYLGYADQGLATVSADAI
                     AADFEATAMGHAILGSPAECIEQLEALVAEFPVDPIIIRAHWPGMSADDAVAIIDEIG
                     RDLIPVLRDVEPSLPPI"
BASE COUNT          905 a         1570 c         1401 g          804 t
ORIGIN      
        1 ggctgaccgg atgatcttgg ctgccctggg ctcgaggtag ccacatgggc gcttgctctt
       61 ctcccccgcc acgttgctcc gctggcatca agagggtgcg caggccttgg tctgccctta
      121 gcctgcgccc acagcgaggc cggccgccga tctcggatga gctgcgggac ctgattctgc
      181 gtttgggtcg cgagaaccca cggtgggggc gatcggcgca tacagggcga gctgctcaag
      241 ctcggctacc acgtctcggc gaccaccatc cgcggcgtcc ttcgccgcca tcgcgttcct
      301 ccagcactcg ccgcgagggc ccgacttggg cgcagtttct catggcttat ctgcccttag
      361 atcaggtcgc caccagtttc ctgttccagc tcgtctccaa gcgccacacc aaaggcagca
      421 tcatcgtcac ctcgaacaag tcctactccg agtgggtgct gttttcggtg acgaagttgc
      481 agccgcggcc atcattgacc gtctgctcca ctactcgacc acggtcaaca tccgtggcga
      541 gagctatcgg ctcaaagaca aaagcgggcc ggcatcttcg ccatgcccac cagcgcacag
      601 cccgccatgt attgggatga aggcgcccac gcaacgccac acttccacgc ccaccatgcc
      661 ggaaggagag catctgtgga cctggagggg cgtgtcctgg cgggcgacat cgacagcgcg
      721 gcgctcggcc acgttcagga atgggctcgc attcatcgag aggaactgct ctcaaactgg
      781 gaccgggcgc gccgctctga accaatcctg ccaatcgaac ccctggaata cgatttgagg
      841 ctatgggcta tcttcctgca gttgttggcg tcgcggtgat cggcgatcac aagctccggc
      901 tgctcttcga tgatggcacc gcaggagacg tcgacctttc caggctggaa tggcgaggtg
      961 tcttcgaacc gctccacgac ccgaaatact tcgcccaggt caagttcgat ccagatgccg
     1021 cgacggtcgt ctggcccaac ggcgcggatc tcgcgcccga aaccctttac gccgaggctc
     1081 aaaaaacccg ctaatcgcag catagccaga tgtcttgatc aacccagagt gaccaagaat
     1141 tggcctacta gcacgggccg gacactctcg agaactgcgg cgaactatgc cgaggcgaac
     1201 gctctgttgg ctgccatttt ggcggcgcgt aatccgaaaa ccaacgcagg cgccagtccg
     1261 ccagcgtagg cgcgcacgta aagcccacct gcatccgcac cagccgccag caggccgggt
     1321 atcgcaacgc cgttctcatc aagaacacgg ccgtgcgagt cggtcagcag cccaccatat
     1381 gtgaaggtga tcgcaggcca cacctcgatc aggtgatagg gcggttcgac cagcggccgc
     1441 gcgtcaaatc gacgcggcag cgacagccct cccgaattga atcttctgat cgagtccact
     1501 accgctcgcc ggtcataacc ccagccgccg gcgacctcac caaccgactc gaggtccgcc
     1561 gccgacgcgt agtgcccacc ccgccgcgcg gcctcaccaa gcttgtcgcc agtttcaatc
     1621 ccatccacgt agggctgcac gatccattcg cgcctcacca gctcgtcggc caccaacaac
     1681 gcccttccac cttgctgctc gagaacggcc tgtgcgctca agtgatcgcc tacggtttcg
     1741 tccacgaacc tccggcccga tttgttcaag agcaggctgt gctcgctgaa gaacatcgtg
     1801 aggatcttga acatcgaagg gtccggcaga tcgaggggaa acgggatcaa gtggccgtaa
     1861 aaacccgcgc ctccctttcc gaattggccg ccggccgcct gtcccagccg caggccgtcg
     1921 ccgctgctgt ggggattcgt gcgccgcggc atcgccgatg cgttgggatg gatcagctgc
     1981 gcggaaagag cgcgatcgtt ctggtagccg ccggtggcaa gcagagtgaa acgcgctcgc
     2041 aacggttgca ccgagccatc actcggccgc acctcggcgc cgaccacggc gccctcttcg
     2101 agcagcagtc gttctgtttg ggccgatacc agcaacttcc catgcggcga atgctggatc
     2161 accccatcgc acgcgcggat ataaccaggc atgtcgaccg ggacgccgcg tccgaagcgc
     2221 agcatcgtaa tcggcttgtg acaattcacg ccgagggact ggatccactc cacgccttct
     2281 ccgatctctg acacaagcgc gcgacccatg tctgcgtcga caccggggac ctcagacatc
     2341 agtgcgtcgg ccgacgcagc cgtccagaca tatccggcca tgctggccga accaccgagt
     2401 tggtcggact tctccacgac cgtcacatga agcccccttt ctgcgctcca ggcggcggcg
     2461 gaaagacctg ccatgccgcc gccgatgacc agcaggtcga gcatcagcga aaaggtcgtc
     2521 gcgccgccag gtccgcctct ttctggctgt cctggtagcg aggcttacgc tgctcgagcg
     2581 ccgcgcggcg ggcttccacg gagtcttcgt agccggagag gtcgcctgtc ctgccttgct
     2641 caaactcgta gccgggcttg aggtcggccc cctcggtttc gttcagggct cgtttgccga
     2701 tccgaagcgc gacggcgctg tggcgagtca gcagcgcggc tttctgtcgc gcgaccccga
     2761 gaagccgttc cggctcgacg acctcgagca ccgcacccaa ggcgacgagc gtgtcgagtg
     2821 gcgccggctc tccgctcaag aacatccagc gagccaccgg ttcggggacg aggcggcgga
     2881 cgtgtgaggc gccgcccata acgccgacgc cgacttccgg caatccaaag cgagcgccac
     2941 gcgctgcgac gatgaaatca caggacgcag ccagcgcaag gccggtgccc agggcggcgc
     3001 cgtgcacggc ggccagtacc gggaccgggc agtcgtagat cgcccagaat gcacgccgca
     3061 cggtcaacat ccggctgggc gcattcgcgg gatccagcgt tccaaactcg accaggtcat
     3121 tgccggcgca gaaatgtttg ccgtcgccag aaaggatcac ggccttgatc ggaccgctta
     3181 gatcggccac cgatgaaaat gcggcatgga tctcttcata gagctcctga ttgactgcat
     3241 tgaccggcgg cctcgccagc acaacatgag cgacggaacc ctcgacttca acgcgaaggt
     3301 gccggtagtc gctcaattcg gaaacacctg cccgtcggtc tcgaatgacc agcggacgga
     3361 gtggacacgc tcagagatct tccatcccgc agcggtcagc acgagccgat cgtgatagcg
     3421 caatccacgc acgcgcatgc gagccgcacc ctgcgcgccg gcgtccgaga caaacgcgat
     3481 cgcataggtt tctgcggtcg caccgtcttc ctcgattgaa acgagcaggt tgccagcgaa
     3541 atgcatcgtc acaccgagcc ggtccattcg cttgcgaagc tcatccgtaa gggcggctgc
     3601 accagagggc atcgcctcgc cgccataagt cgcagtcacg ttttcgtcga agcagtccga
     3661 caccatgtcg tagtcgcggc gatcgagggc atggaagtac cgaagcacca cattgcgcac
     3721 cggctcgtcg gccgctagca ttaaatcatt ctaatgaatc gcagcgattc gaagcgagcg
     3781 atgctggtcg tcgggatcga cgttgatcct gagcacgagg aggagctgaa tcgctggtac
     3841 gactccgaac acattccgca gcgcctcggc atccccggct tcgttcgagc ccgccgtctg
     3901 gtgagagccg ccgcgcccca tacgagcggg aatctgaaag gcacacccga catgccgcgc
     3961 taccttgccc tgtacgaggt caccgacccg aaagtcttcg agaccgtggc gtaccgcaag
     4021 ctcgtcgaca accccacgcc ctggacgatc cgcatgcgcg ataccttcga gctgcggatt
     4081 cggggcatct atgtcgaaac gctgagcatg tcggcagacg accctgcggt cgaacctttc
     4141 agggtcagat aggtggcaga gaaggctcga catcacgcag aacagggatc aggtcacggc
     4201 cgatctcgtc aatgatcgcg actgcgtcat cagcgctcat acccggccag tgtgctcgga
     4261 tgatgatcgg gtcgaccgga aactccgcga caagcgcctc caactgttcg atgcactcgg
     4321 ccggggatcc gagaatcgca tgacccatcg ccgtcgcctc aaaatccgcg gcgatggcat
     4381 cggcggatac ggtggcgagc ccctggtccg cgtagccgag gtagcgatcc tgggccatcg
     4441 agcggaagcg gcggagggcg tcatcccggt cacggccgat cgtgacctcg cggcgcaatg
     4501 gctgccgtcc cattgggaga ccggcttcac ggcgcgcttc gaagaaagta cgaaggcgca
     4561 cttcgatctc tgcatggggc agccgggggc taataggcca tgcgtcgccg attcttgccg
     4621 cgcgcctgat cccgggcgcg gaatgggctc cgatccacag gggtggtcgt ggcttctgca
//
LOCUS       DATGEM010000082        13055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_885979, whole genome shotgun sequence.
ACCESSION   DATGEM010000082 DATGEM010000000
VERSION     DATGEM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13055
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885979"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VKQ27_02665"
     CDS             complement(<1..1241)
                     /locus_tag="VKQ27_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HLY87865.1"
                     /translation="MGDFDAIIIGAGQAGPSLAGRLSSAGMTVALIERHLFGGTCVNT
                     GCMPTKTLVASAYAAHLARRSADYGVLFDGPVRIDMARVKARADAVSRNARTGVESWL
                     RGMKGCTVLQGAARFVSPDTVAMGSERLRARRIFVNVGGRAIVPELPGVDSVNTLTNR
                     SMLALEEVPDHLLVIGGSYIGLEFAQMYRRFGARVTVIEQAPRLVMREDEEISASIRE
                     ILEDEGITVRTGAQCIRIGPHAAGVAVAVDCGSGAPEVVGSHLLLAVGRRPNTDDLGL
                     DKAGIATDARGYIRVDDQLATNVPGIWALGDCNGRGAFTHTAYNDFEIVAANLLDGET
                     RRVSQRIPAYALFIDPPLGRVGISETEARASGRPLLVGRRPMTRVGRAVEKDETKGFM
                     KVVVDAETSKILGAAILGTGG"
     gene            complement(1265..1942)
                     /locus_tag="VKQ27_02670"
     CDS             complement(1265..1942)
                     /locus_tag="VKQ27_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87866.1"
                     /translation="MSSAAVETPKARGMDMKFEIVVIPVSDVDRAKLFYDGLGWRLDA
                     DYASDDGYYRVIQFTPPGSGCSVIFGKNVTAAAPGSAQGLYLIVSDVEAARKELRGRG
                     VDVSAVFHDAAGAYAGPDEPYLFGRVRVDGRDPEHRSYRSFASFSDPDGNGWLFQELT
                     HRLPGRLDSGATTFASLADLSSALRRAEAAHGEHEKRTGGQRDENWPDWYAEYLIHEQ
                     AGTELPL"
     gene            complement(2041..7509)
                     /locus_tag="VKQ27_02675"
     CDS             complement(2041..7509)
                     /locus_tag="VKQ27_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY87867.1"
                     /translation="MGVGGDSQALEVLWEEAERVFCRLRQDDAQGHAYAFIPVVPDGG
                     HSTIETINRFAHEYELKSYLDREWALPPVAFVRDPARTMLVVDYAGGQPLDHLIGKPM
                     EVGQFLRLAVALSAALGRLHRCGLIHKDIKPANVLADCATGRVWLTGFGIASRLPRER
                     PSPAPPEFIAGTLAYMAPEQTGRVNRSVDSRSDLYSLGVTFYEMLTGRLPFSGSEPME
                     WVHCHIARQPKAPGEVLNDVPPTVSAIIMKLLCKTVEDRFQTAGGVENDLRRCLSAWE
                     ALGRIDDFAPGENDTPDRLLIPERLYGRDREIDTLLTAFDHIVAGGRPELVLVSGYSG
                     IGKSAVVNELHKPLVPPRGLFASGKFDQYQRDIPYATLAQAFQSLIRPLLSKKEEDLR
                     LWRDALREALDPNGLLIVELVPELKHIIGAQPPVPELPPQEAQRRFQLVFRRFIGVFA
                     RREHPLALFLDDLQWLDTATLDLLEDLLTRTDLQYLLLIGAYRDNEVNPTHPLIRKLT
                     AIRQAGAAVQDIVLTPLGRDDITRLLADSLHCEPERAAPLAVLIREKTTGNPFFAIQF
                     ISTLVDEGLLTFDYGDGHWVWDLRRIRAKGYTDNVVELMVGKLNRLPEETRGALQQFA
                     CMGNRAEFEMLSLVYQGSVEDMHARLWEAVRSGLIFRADDSYRFLHDRVQEAAYSLIP
                     MEQRTEAHLRIGMLLAAHTPPAKREEAIFEIVNQLNRGAHLIASVEEREHVAGLNLIA
                     GRRAKLSTAYDSALKYLTAGLALLSDETWGRNYDLIFSIEYLMAECELLTADKTSAES
                     RMSHLAQRARNRHDVCVVTRLRLTLYTTLDRSDLGVDVFLDWLRRDGTVWSNHPSADD
                     VMYEYTRIWSLLGDRQIEDLIDLPLITDRDVLDTLDVFTEIMTPAQLFDENLSSLVIC
                     RMVILSLEHGNCDASCFCYVWLAMFAGPRFNNYQGGFRFGQLGYELVEKRGLTRYQAR
                     IWMNMGSTVLPWAKHPASGRELVRRAFDAAYRIGDLTFASYSWDQLVTICLAAGDPLA
                     EVQTECENGLAFAKRVRFGLVVELCGAQLGLILTLRGVTPIFGCLDHEDFTEPNNDLA
                     SNPNLVFAQFYSWTRKLQARFFAGDIDAAIDAALNAEPLLWTSAAMFESAEYRLYGGL
                     AHAAAWDSAAPDQKLKHFAAVTNHHRQLKVWAKHNPATFENRAAIVGAEIARIEGRTL
                     DAQELYEKAVRSARENGFLHNEGLANELAGRFYAARGFEKIATVYLRDAHYCYLRWGA
                     LGKVRQLEQLYPHLRADKIVSDPTSTILAPIEHLDLATVIKVSEAVSGEIVLDKLIDI
                     LVRTAIEHAGAERGLLILPRDDAYRIEAEISISGNRMTVSLRQSEVTGTDLPESVFRY
                     SLRTKEYLLLHDALTHSPFSEDDYIRRNNSRSVLCLPIIKQNRLLGMLYLENSLTPGA
                     FTPARMAVLKLLASEAAISIENARLYQELAEREARIRRLVDANIIGIFFWDFQGRILE
                     ANDAFLRMVGRDREELARRPVHWKDLTPSDWLARNEEPPVETDFYRKDGNRVPVLIGV
                     ATFEEGGYQGVAFVLDLTERKRAADTLRALQMDLAHANRLATLGHLVASLAHEVNQPI
                     GAVRNNAHAALRFLAGNPPNLAEVSEALECMVHETYRVGDIINGIRDQVRKAPPRNEN
                     IDLNDAIGEVIAIVRGELSRHHVSVQVLLAHDLPPVYADRVQLQQVMLNLILNAIEAM
                     ISVDDDARELLVSTESTPADGALVAVSDSGPGVALEDRERVFESFYTTKGGGLGIGLS
                     ICRSIIDAHGGRLWVEARQPRGAAFRFTLPAHNQSAGR"
     gene            complement(7539..7925)
                     /locus_tag="VKQ27_02680"
     CDS             complement(7539..7925)
                     /locus_tag="VKQ27_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87868.1"
                     /translation="MPEQSQISVVDDDQSFRDSLRRLLRSLNYDVAVFPSAADFLASS
                     RFSETACLVADVHMPAMTGIELFEHLIKTGHAIPTILITAYPDDDARKRLLDLGVECY
                     LLKPLVEAVLINCLRSAVARGKMSQG"
     gene            8178..8813
                     /locus_tag="VKQ27_02685"
     CDS             8178..8813
                     /locus_tag="VKQ27_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY87869.1"
                     /translation="MPESNPCVVVIDDDREFSQSLGRLLRSVGLESRLFASVADFVVS
                     DPPDRPTCLVLDVRLPERSGLDFQRDLVAAGVTLPIVFITGHGDIPMTVQAMRAGAIE
                     FLTKPFREQDILDAINVGLGRDRTRRDQEKALGALRAQFGTLSPRERAILNRVVEGRL
                     NKQIAGEMGIAESTVKVHRSNIMRKVNATSVPALCRMVDKLKLSPGSPEPS"
     gene            complement(8985..9950)
                     /locus_tag="VKQ27_02690"
     CDS             complement(8985..9950)
                     /locus_tag="VKQ27_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLY87870.1"
                     /translation="MSRRSIAIVIHDGVQALDVAGPLDVFAEANGFVDREQGYDITVI
                     GPEARPLRSSNGLSLVADLVFDQATTAFHTALVAGGPDLPTRPDDPAMSTWLRNWGTR
                     AERYGSICTGAFALGHAGLLDGRRVTTHWQNAHHLAALFPKARVEHDRIYLRDEALVT
                     SAGVTAGIDLALALVAEDHGAAVALSCAKRLVVVAQRHGGQSQFSPYLLPPAAPESPL
                     AKVQAYVMSHPGESFPVERLAQIAGISARSVARLFTAELGVTPHDFVESVRIDRARNL
                     LEGSDKALKAIAYDCGFGGSEQMRAVFQRRLGVSPVQYRASFRTG"
     gene            10063..10755
                     /locus_tag="VKQ27_02695"
     CDS             10063..10755
                     /locus_tag="VKQ27_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLY87871.1"
                     /translation="MDAGALMVRRNRLQEVSMIQSIGGVAIPDSKLAREISDFVRDTE
                     TELLFNHSSRVYLFGALAGEQRGLTYDAELLYAAAMFHDVGLMHEHSSDHLRFEVDGA
                     NAARDFLKSRGIAPADLDTVWTAIALHTTPGVPEFMHPVIALTTAGVEMDVLGLTYGQ
                     YPAEIREAVVAAFPRTPHFKEDIIQAFYDGIKHKPDTTFGNVKADVIADKEPHFHRGN
                     FCSVIRGSHWHG"
     gene            10824..11273
                     /locus_tag="VKQ27_02700"
     CDS             10824..11273
                     /locus_tag="VKQ27_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY87872.1"
                     /translation="MPSPETDEKPRLDDFLCFAIYSANLAYGRAYKPFLDELGITYTQ
                     WIAIVALWEKGDQTVSGLGEILFLESNTLTPILKKLEAEGYLTRKRDPADERQVRVSL
                     TEAGRSLREKGLHRSLVAATGLPPDEFQKVQKTVKTIRDNLTRHTGR"
     gene            complement(11251..11982)
                     /locus_tag="VKQ27_02705"
     CDS             complement(11251..11982)
                     /locus_tag="VKQ27_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLY87873.1"
                     /translation="MAGPLRIGTAGWSIPTEFAADFPGQGTHLERYAKRFNAVEINSS
                     FYRPHRRTTYERWADSVPADFRFSVKLPKSLTHERRLKDGEDLVERFAGEVEGLGEKF
                     GVLLIQLPPSLAFSDEAGDFLRRLRPRFAAALALEPRHKSWFMPAVEEFLTESGIARV
                     AADPPRFAGGDEPAGCRKTAYFRLHGSPRIYYSRYDEAALADWSRKASHAVKTGAQAW
                     VIFDNTAEGAAIDNAAEMITGRYGG"
     gene            complement(12081..>13055)
                     /locus_tag="VKQ27_02710"
     CDS             complement(12081..>13055)
                     /locus_tag="VKQ27_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY87874.1"
                     /translation="MQGAQRAGSLTSRLLAFSRQQPLDPKATELDQLIFAMSEILHRT
                     LGEQITLEAVLPPDLWRVEIDQNQLESALLNLAVNARDAMTEGGKLTIAAKNTVLEDS
                     HATFHIEPGEYVAISIGDTGHGMSHETVARAFDPFFTTKSAGHGTGLGLSMVYGFAKQ
                     SGGHITIDSAIGRGTTVTLYFPRYYGAIEAAPAIGDQSAPHARDGEVVLVVEDNDDVR
                     FYGTSILSELGYRVLEATNSTEALSVIGQDRRIDLLFTDVILPGMSGKAMADQAKSLR
                     PDLKVLFATGYSRDAIVHHGRIDPGVNLLLKPFTYEQVASRVREILDR"
BASE COUNT         2471 a         4077 c         4096 g         2411 t
ORIGIN      
        1 tcgccgccgg tacccaggat ggccgcaccg aggatcttcg acgtctccgc gtccacgacc
       61 accttcatga agcccttcgt ctcgtccttc tcgacagcgc gccccacacg ggtcatcggc
      121 cgacgcccga cgagcagggg ccggccggac gcgcgcgcct cggtctccga gattccgacc
      181 cggccgagcg gggggtcgat gaagagagcg taggcgggga tccgctggct cacgcggcgg
      241 gtctcgccat ccaggaggtt ggccgcaacg atctcgaagt cgttataggc cgtgtgtgtg
      301 aaggcgcctc ggccattgca gtcgccgagc gcccagatgc cgggcacatt ggtcgcgagt
      361 tgatcgtcga cgcggatata gccgcgcgcg tcggtcgcga taccggcctt gtcgaggccc
      421 aaatcgtccg tattgggtcg acggcccacg gccagaagga gatggctgcc gacgacctcc
      481 ggtgccccgc tcccgcagtc gaccgcgacg gcgacgcctg ccgcgtgcgg cccgatgcga
      541 atgcattggg cgccggtacg caccgtaatt ccctcatcct cgagaatttc ccggatcgaa
      601 gccgagatct cctcatcctc gcgcatgacg aggcgcggcg cttgttcgat gactgtcacc
      661 cgggcgccga accggcggta catctgggcg aattcaagcc cgatatagct gccgccgatc
      721 accagcagat ggtcgggtac ttcttcgagc gccagcatcg acctgttggt cagggtgttt
      781 acgctgtcga cgcccggcag ctccggcacg atggcacgtc cgccgacatt gacgaagatg
      841 cgccgcgcgc ggaggcgttc ggaacccatc gcaaccgtgt ccggcgacac aaaacgcgcg
      901 gcgccctgca gcaccgtgca ccctttcatc ccgcgcagcc agctctcgac gccggtgcgc
      961 gcgttgcgcg acaccgcgtc cgcgcgcgcc tttacccgcg ccatgtcgat ccgaaccggt
     1021 ccatcgaaca ggacaccata gtcggcgcta cgccgcgcga gatgcgcggc gtaggcgctg
     1081 gcgacgagcg tcttggtcgg catgcagccg gtgttgacgc aggttccccc gaagaggtgg
     1141 cgctcgatga gtgcgacggt catgccggcg ctgctcagcc ggcccgcgag ggatggaccg
     1201 gcctgcccgg cgccgatgat gattgcgtcg aaatcgccca tgatgagtgc tctcccgtgc
     1261 cagcttacag aggcagctcc gtgcccgcct gctcgtgaat gaggtactcg gcgtaccaat
     1321 ccggccagtt ctcatcgcgt tggccgccag tccgcttctc gtgctcgcca tgcgccgcct
     1381 ccgcccgccg cagcgcgctc gagagatccg ccaacgaggc gaaggtcgtg gcgcctgaat
     1441 ccaagcggcc gggcagccgg tgggtgagtt cctggaacag ccagccattg ccgtccggat
     1501 cgctgaacga ggcgaacgag cgatagctgc gatgctcggg atcccggccg tcgacccgga
     1561 ctcgcccgaa caaatagggt tcatccggtc cggcatacgc gccggcggcg tcatggaaca
     1621 cggcgctgac gtccacgccg cgaccgcgga gctctttgcg ggccgcttcg acgtcggaga
     1681 caatcagata tagtccctgg gcggaaccgg gcgcggccgc ggtgacgttc ttgccgaaga
     1741 tgacggagca cccggagccg ggcggggtga actggattac gcgataatag ccatcatccg
     1801 aggcgtaatc ggcgtcaagc ctccatccca ggccgtcgta aaagagcttg gcgcgatcga
     1861 cgtccgatac ggggatcacg acgatctcaa acttcatatc catgccccgc gccttcgggg
     1921 tctcgactgc agcgctgctc atgtaaacct ccgatattca gggtctccga cacgctcgtt
     1981 ccgagttttc tgcgatctac tagagcagaa ggctgaggct tacttcaatt aggccatggc
     2041 ttatcggccg gcactttggt tgtgcgccgg tagagtaaag cgaaaggcgg cgccgcgagg
     2101 ttgacgcgcc tcgacccaca atcgtccgcc gtgggcgtcg atgatggagc ggcatatcga
     2161 gagcccgatt cccaatccgc cgcccttagt ggtgtagaag gactcgaaaa cgcgctcgcg
     2221 atcctccagg gcgacacccg gccccgaatc gcttaccgca acgagcgcgc cgtcggccgg
     2281 agtagattcg gtgctgacca gcagttcacg cgcgtcatcg tcgacactga tcatggcttc
     2341 gatcgcgttg aggatcagat tcaacatgac ctgctgcagc tgaacccgat ccgcgtaaac
     2401 cggcggcaaa tcgtgtgcga gcaggacctg gaccgaaacg tgatgcctgg atagctcgcc
     2461 tcggacgatc gcaatcactt ctccgatggc atcgttcaga tcgatgttct cattgcgagg
     2521 aggcgctttt ctgacttggt cgcggatccc attgatgatg tctcccaccc gataggtctc
     2581 gtggaccatg cattcgagtg cctcggagac ttccgccaag ttcggcggat ttccggcgag
     2641 gaagcgcaag gcggcatggg cgttgtttcg gaccgcgcca atcggctgat tgacttcgtg
     2701 ggcaagggag gcgacgagat gccccaaagt cgccaggcgg ttggcgtggg cgagatccat
     2761 ctgtaacgcc cgtaacgtgt cggcggctcg cttgcgctcg gtcaggtcaa ggacgaacgc
     2821 gaccccttgg tagccgcctt cttcgaacgt cgccacaccg atcagcacgg gcacccggtt
     2881 accgtctttt cggtagaagt ccgtttcgac cggcggctct tcattgcggg cgagccaatc
     2941 ggatggagtg agatccttcc aatggacggg tctgcgggcg agttcctcgc ggtcgcgtcc
     3001 caccatccga agaaaagcgt cattggcctc gagaatgcga ccttggaagt cccagaagaa
     3061 gatcccgata atattggcgt cgaccagtcg tcggatcctg gcctcgcgtt cggccagttc
     3121 ctggtagagg cgcgcgtttt cgatcgagat ggccgcctcg gaggccagaa gtttgaggac
     3181 cgccattcga gccggcgtaa aggcgcccgg tgtgagactg ttctccagat acaacatccc
     3241 gagcagtcta ttttgcttga ttatcggcag gcataacact gaccgagaat tgttccgacg
     3301 gatatagtca tcttcagaaa acggactatg ggtcagcgca tcatgcaaca aaagatattc
     3361 tttggttctc agggagtatc ggaaaacgga ttctggcagg tccgttcccg ttacttccga
     3421 ctgccggagg ctgacggtca tacggttgcc gctgatgctt atctcggctt cgatccgata
     3481 tgcgtcatct cgcggaagga tcagtagacc tcgttcggcc cccgcatgct cgatcgccgt
     3541 gcgcacgaga atatcgatga gcttgtccag gacgatttcg cccgacacgg cctcggagac
     3601 cttgataacg gttgccagat cgagatgttc gatcggggcc agtatcgtgg atgtcggatc
     3661 tgatacgatt ttgtccgctc tgaggtgcgg ataaagctgt tcgagctggc gaacctttcc
     3721 cagagcgccc cagcgaaggt agcagtagtg cgcgtcgcgc aggtagaccg tcgcgatctt
     3781 ctcgaacccg cgggccgcat agaagcgccc ggcaagttcg ttcgcgagcc cctcattgtg
     3841 gagaaacccg ttctcgcgtg ccgagcgcac ggctttctca taaagctcct gcgcgtcgag
     3901 cgtacgacct tcaatccgcg cgatctccgc gccgacgatg gccgcacggt tttcgaaagt
     3961 cgccgggttg tgcttggccc acaccttgag ttgccgatgg tggttcgtca ccgccgcgaa
     4021 gtgcttcagc ttttggtcgg gagccgctga atcccaagcg gcggcatgag caagcccgcc
     4081 atatagccga tactctgcag attcgaacat tgccgcggat gtccacagca atggttccgc
     4141 attcagagcg gcgtcgatcg ccgcatcgat atcgccggca aagaagcggg cctgcaactt
     4201 gcgagtccag gagtagaact gagcaaacac gagattgggg ttgctcgcca aatcgttgtt
     4261 cggctccgtg aagtcctcat gatccaggca gccgaaaatc ggggtcacac cgcgcagggt
     4321 tagtatcagg ccgagctgcg ctccgcagag ttcgacgacc aggccgaaac gcacccgctt
     4381 cgcaaacgcc aagccgttct cgcattccgt ttgaacttcc gcgagaggat ccccggccgc
     4441 cagacagatt gtgaccagtt gatcccagct atacgacgcg aaggtgaggt cgcctattcg
     4501 gtacgcggcg tcaaacgcgc gccggacgag ttcgcggccg ctggcggggt gtttcgccca
     4561 gggcaaaacg gtcgacccca tattcatcca gattcgcgcc tggtaacgcg tcaagccgcg
     4621 cttttcgacc aattcatagc ccagttggcc gaaccgaaag ccgccttggt aattattgaa
     4681 gcgcgggccg gcaaacatcg ccagccagac atagcaaaag cacgaagcgt cgcaattgcc
     4741 atgctcgagg ctgagaatca ccatgcggca gatgaccagc gatgacaggt tttcgtcaaa
     4801 aagctgcgcg ggcgtcatga tctccgtgaa gacatccagc gtgtcgagga catcacggtc
     4861 cgtgatcaga ggcagatcga tgaggtcttc gatttgccga tcgccgagta gagaccagat
     4921 gcgtgtgtat tcgtacatga catcgtcggc gctcggatgg ttcgaccaca ccgtcccgtc
     4981 gcggcggagc caatcgagaa acacgtcgac ccccaggtcg ctccgatcca gggtggtgta
     5041 gagggtgagc cgcaagcgcg ttacgacaca gacgtcatgt cggtttctgg cgcgttgcgc
     5101 cagatgcgac attcggctct cggccgatgt cttgtctgcg gtcagcagct cgcactcggc
     5161 catcaggtac tcgatagaga agatgagatc gtaattgcgc ccccatgttt cgtccgagag
     5221 cagcgcgaga ccggccgtga gatatttgag cgcggagtcg tatgcggtcg agagctttgc
     5281 gcgcctgccc gcaatcagat tcaggcccgc gacatgctcg cgctcttcga ccgaggcaat
     5341 gaggtgcgcc ccgcgattca gctgattgac gatttcgaaa atcgcctctt cgcgtttcgc
     5401 cgggggcgta tgggcggcca gcagcatgcc gatgcgcagg tgagcctcgg tgcgctgctc
     5461 catcgggatc agcgagtacg ctgcttcctg cacgcggtca tggaggaaac ggtaggagtc
     5521 gtcggcccgg aagatgaggc ccgagcgcac ggcttcccag agacgcgcgt gcatgtcctc
     5581 gaccgagccc tgataaacca agctcagcat ctcgaactcg gcacggttgc ccatgcaggc
     5641 gaactgctgg agcgcccccc gggtctcctc cggcaggcga ttcagcttcc ccaccatcag
     5701 ctcgaccaca ttgtccgtat agcctttggc gcgaatgcgg cgcaggtccc aaacccagtg
     5761 tccgtcgccg tagtcgaacg tcagcaggcc ctcatcgacc agtgtggaaa tgaactggat
     5821 ggcgaaaaac gggttgcccg tcgtcttttc gcgaatcagc accgcgagcg gtgccgcgcg
     5881 ctccggttcg cagtgaaggc tatccgccag caaccgggtg atgtcgtcac gcccgagcgg
     5941 cgtgagaacg atatcctgca cggccgctcc cgcctggcgg attgccgtga gtttgcggat
     6001 caacgggtgg gtaggattga cctcgttgtc ccgataggcg ccgatgagca acaggtactg
     6061 gagatccgtg cgggtcaata gatcttcgag caagtcgagt gttgccgtat ccagccattg
     6121 caaatcgtcg aggaagagcg cgaggggatg ttcccgacgg gcgaataccc cgatgaaccg
     6181 tcgaaagacc agctggaacc gtcgctgcgc ctcttgcggt ggcagttcgg gcaccggcgg
     6241 ctgcgcgcca atgatgtgct tcagttccgg aaccagctcg acgatgagca gtccgttcgg
     6301 atccagcgct tcgcgcagcg catcgcgcca taagcgcaga tcctcttcct ttttgctgag
     6361 gagtggccgg ataaggctct gaaaggcctg ggccaatgtg gcatagggga tgtcgcgctg
     6421 atattgatcg aacttgcccg aggcgaataa gcctcgaggg ggtacaagcg gtttgtggag
     6481 ttcgttcaca accgcggact tgccgatccc ggagtagccg gaaacgagca ccagttcggg
     6541 tctgccgccg gcgacgatat gatcgaacgc ggttagaagc gtgtcgatct cacggtcgcg
     6601 tccatacaat ctctcgggaa tgagcaggcg atccggcgtg tcattttcgc ccggcgcgaa
     6661 atcgtcgatg cgccccagtg cctcccacgc cgagaggcag cggcgaagat cgttctccac
     6721 gccccctgcc gtctgaaagc ggtcttcgac ggttttgcaa agaagcttca tgatgatcgc
     6781 ggagaccgtc ggcgggacgt cgttcaggac ctcgccgggt gctttcggct gtctggcgat
     6841 atggcagtgc acccattcca tcggctcgga tcccgagaac ggaagacggc cggtcagcat
     6901 ctcgtaaaag gtgactccaa gcgagtaaag gtcgctccgg gagtcgacgg aacggttcac
     6961 ccgcccggtt tgctcgggtg ccatataagc gagcgttcct gcgatgaact cggggggggc
     7021 gggcgagggg cgctcgcgcg ggagacgaga cgctatgccg aagccggtga gccagactcg
     7081 ccccgtcgcg caatcggcca gcacattggc aggcttgatg tccttatgga taagcccgca
     7141 tcggtggagc cggccaagtg ccgcggacag ggccaccgcg agccgcagaa actgtcccac
     7201 ttccatgggc ttgccgataa ggtggtcgag cggctggcct cccgcgtagt cgacgaccag
     7261 catcgtccgc gcggggtcgc gcacgaacgc caccggcggc aacgcccact cgcggtcgag
     7321 gtagcttttg agttcgtatt cgtgggcgaa acgattgatg gtctcgatcg tcgagtgccc
     7381 gccgtcggga acgacgggga tgaacgcata cgcgtggccc tgggcgtcat cctgcctcaa
     7441 tctgcagaag acgcgttccg cctcctccca caagacttct aaagcctgac tgtctccacc
     7501 aacgcccatc ctgcagtctc ctgcaaattg tcaggtgatc agccttgcga cattttaccg
     7561 cgcgcgaccg ctgaccgcag gcaattaatc aggacggcct cgaccaacgg cttaaggaga
     7621 taacactcga cacccaggtc caacaggcgc ttccgcgcgt catcatccgg ataggcggtg
     7681 atgaggatcg tcgggatcgc gtgccctgtt ttgatcagat gttcgaaaag ctcgatgccc
     7741 gtcattgcgg gcatatggac atcggcgacc aggcaagccg tctcggaaaa tctcgacgac
     7801 gcgagaaaat ccgcggcgga ggggaagaca gcgacgtcat agttcagcga tctgagcagt
     7861 cttcgcaggg agtctcggaa ggattggtcg tcgtcaacga ctgagatctg tgattgctca
     7921 ggcaatgagg cctccggttc gtcacgcgcg gcgacaactt tacccgagag agttcataga
     7981 gcaccgccta tgccactaag aatagcgcat taacgatcct cgatcgctaa accatggttt
     8041 aggccgctag gcccttggaa cccggcttcg ctctatccga tgagatccgt tttcagtagt
     8101 atagacactg agacggcaac agaacttccg cgagggccaa gcctccccgc gaagttcttc
     8161 aatcgcgagc gcgagtgatg ccagagtcaa atccttgcgt ggtcgtcatc gacgacgatc
     8221 gggagttcag ccaatcgctg gggcggctgt tgcggtccgt cggcctggag tctcgactgt
     8281 ttgcgtcggt tgccgatttt gtggtgtccg atccgcccga taggccgact tgcctggttc
     8341 tcgatgtgag attgccggag cggagtgggc tggattttca gcgagatctc gtcgcagcgg
     8401 gcgtcacact cccgatcgtc ttcatcaccg gacatggcga catcccgatg accgtgcagg
     8461 ctatgagggc gggtgcgatc gagtttttga cgaagccgtt ccgggagcag gatattctcg
     8521 atgccataaa tgtcgggctt ggccgcgacc gtacgcgccg cgaccaggaa aaagccctcg
     8581 gcgcgctcag ggcgcaattc ggaacattgt cgccccgtga acgcgccatc ctgaaccggg
     8641 tcgtggaggg ccggctgaac aagcagattg ccggcgagat gggcatcgca gaatccacgg
     8701 tgaaggtcca ccgaagcaac atcatgcgga aggtcaacgc cacgtcggtc ccagcactct
     8761 gccggatggt ggacaagctg aagttatcgc cgggaagccc cgaaccttca tgagagagca
     8821 tggctcaaaa tcccgaggca ccaacacttt ggttttttgc acagcctggc gtctcgacac
     8881 tagggatctc cgcgcattct gctgacggtg atcgccaaaa accccaacgc ggtcgaggac
     8941 gcgctctttc cgggtgcgcc attctctcat cagcgaaaaa tctctcagcc tgtcctgaag
     9001 ctggcgcgat actgcaccgg gcttacgccc agtcttcgct gaaagaccgc gcgcatctgt
     9061 tccgagccgc caaagccgca gtcataggcg atggctttca acgccttgtc cgatccttcg
     9121 agcaggttgc gcgcccggtc gatgcgcaca ctttccacga aatcatgcgg cgtcacgccg
     9181 agctccgcgg tgaacaggcg ggccacgctc cgggcgctga tgccggcgat ctgcgccagc
     9241 cgctcgaccg ggaaggactc gccgggatgg ctcatcacat aggcctgcac cttggccagc
     9301 ggcgattcgg gcgccgcggg cggcaggaga taggggctga attgcgactg cccgccgtgg
     9361 cgctgggcca ccaccaccag ccgtttggcg caggatagcg ccactgccgc gccgtgatcc
     9421 tcggcgacca gggccaacgc cagatcgatt cccgccgtga ctccggccga ggtgaccagc
     9481 gcctcgtcgc gcagatagat gcggtcgtgc tccacccgtg ccttgggaaa cagggcggcg
     9541 aggtgatgcg cgttctgcca atgggtggtc acccggcggc cgtcgagcaa tccggcatgg
     9601 ccgagggcga aggcgccggt gcagatcgag ccgtaacgct ccgcccgcgt gccccaattc
     9661 cgcagccagg tactcatcgc cggatcgtcc ggccgggtcg gcagatcggg gccgccggcg
     9721 accagggcgg tgtgaaaggc ggtggtggcc tgatcgaaaa ccagatccgc caccaggctg
     9781 agcccgttcg acgagcggag cggccgggct tcggggccga tcaccgtgat gtcgtagccc
     9841 tgctcccggt cgacgaaacc attggcttcg gcgaatacgt cgagcgggcc ggcgacatcg
     9901 agcgcctgca ccccgtcatg gatcacaatg gctatggatc gtcggctcat cggcgccact
     9961 atcccaaccc gatccctgct ccgcaaggca ggaaatcggg ggcgattggc agaatccgca
    10021 ccctggcccg cgttgcggcg aaacagctcg ccggcgcata agatggatgc cggagccctg
    10081 atggtccggc gcaaccggct tcaggaggtt tccatgatcc aatccatcgg cggcgtcgcc
    10141 atccccgaca gcaagctcgc tcgcgagatc agcgacttcg ttcgcgatac cgagacagag
    10201 ctgctgttca accattcgag ccgcgtctat ttgttcggcg cgctggccgg cgagcagcgc
    10261 ggcctgacct atgacgccga attgctctac gcagcggcca tgttccacga tgtcggtctg
    10321 atgcacgagc acagcagcga tcatctgcgc ttcgaggtcg acggcgccaa tgccgcccgc
    10381 gacttcctca agagccgcgg aatcgcgccg gccgatctcg atacggtgtg gactgcgatc
    10441 gcgctccaca ccacccccgg cgttccggaa ttcatgcatc ccgtcatcgc cctgacgacc
    10501 gccggggtcg agatggatgt gctgggcctg acctacggcc agtatccggc cgagatccgc
    10561 gaagcggtgg tcgccgcctt cccgcgcacg ccgcatttca aggaggacat catccaggcc
    10621 ttctatgacg gcatcaaaca caagcccgac accacctttg gcaatgtcaa agccgacgtc
    10681 atcgccgaca aggagccgca tttccaccgc gggaatttct gcagcgtgat ccgcggttcc
    10741 cactggcacg gctgatataa tcgtatgcga tttaatcgcc gcatactata ttagggggag
    10801 cgaactcctt ccggatgaca gagatgccga gccccgagac cgacgagaag ccccgcctgg
    10861 atgatttcct gtgcttcgcg atctattccg ccaatctggc ctatggacgc gcctataagc
    10921 cgtttctcga cgagctcggc atcacctaca cgcaatggat cgccatcgtc gcgctgtggg
    10981 agaaggggga tcagacggtc agcgggctgg gcgagatcct gttcctggaa tccaacaccc
    11041 tgaccccgat cctgaagaag ctcgaagcgg agggctatct gacccgcaag cgcgatccgg
    11101 cggatgaacg tcaagtgcgg gtcagcctga ccgaggccgg gcgcagcctg cgcgaaaagg
    11161 ggttgcatcg ctctttggtg gcggccacgg gcctgccgcc cgacgagttc cagaaggttc
    11221 agaagacggt caagacgatc cgcgacaatc tcacccgcca taccggccgg taatcatctc
    11281 agccgcattg tcgatcgccg caccctccgc ggtgttgtcg aagatcaccc aggcctgcgc
    11341 gccggttttg acggcatggc tcgctttgcg tgaccagtcc gccaacgccg cctcgtcata
    11401 acgcgaataa tagatgcgcg gcgagccgtg caggcggaaa taggccgtct tgcggcagcc
    11461 ggccggctcg tccccgccgg caaagcgggg cggatcggcg gcgacgcggg cgatgccgct
    11521 ctcggtcagg aattcctcca ctgccggcat gaaccagctc ttgtgacgcg gctccagagc
    11581 cagcgcggcc gcgaagcggg ggcgcagccg gcgcaggaaa tcgccagcct cgtcgctgaa
    11641 tgccaggctg ggcggcaatt ggatcagcag gacgccgaat ttctccccca gcccttccac
    11701 ttcgccggcg aagcgctcga ccagatcctc gccgtccttg aggcggcgct catgggtaag
    11761 gctcttgggc aatttgacgg aaaagcggaa atccgccggc acgctgtcgg cccagcgctc
    11821 ataagtcgtc cggcgatgcg ggcggtagaa gctgctgttg atctcgacgg cattgaaacg
    11881 ctttgcatag cgctcgagat gggtgccttg gcctggaaaa tccgccgcaa actcggtcgg
    11941 aatcgaccag cccgcagtgc cgatgcgcag cgggccagcc attacatttg gcgggagagc
    12001 ctcccccgca ccctcggggt cgtcgcgatg ctcctccgag cgggatgaag tttttcgatg
    12061 caatgaaatg ccatcccgcc ctagcgatcc agaatctcgc ggacgcgcga cgccacctgc
    12121 tcgtaggtga agggcttcag cagcagattg acacccggat cgatccggcc gtgatggacg
    12181 atggcgtcgc gggaatagcc ggtggcgaac agcaccttga ggtcggggcg caggctcttg
    12241 gcctggtcgg ccatggcttt gccgctcatg ccgggcaata tcacgtcggt gaacagcaga
    12301 tcgatgcgcc ggtcctgtcc gatcaccgac agcgcctcgg tgctgttggt ggcttccagc
    12361 acgcgatagc ccagttcgga cagaatcgag gtgccgtaga accggacatc gtcattatcc
    12421 tcgaccacca gcaccacctc gccgtcgcgc gcatggggcg cgctttggtc gccgatggcg
    12481 ggggcggcct cgatggcgcc gtaataacgc gggaaataga gcgtcaccgt ggtgccgcgc
    12541 ccgatcgcgc tgtcgatggt gatatgcccg cccgattgct tggcgaagcc atagaccatc
    12601 gacaggccga gcccggtgcc gtggccggcc gacttggtgg tgaagaaggg gtcgaaggcg
    12661 cgcgccaccg tctcgtgcga catgccgtgg ccggtatcgc cgatcgagat cgccacatac
    12721 tcgccgggct cgatatgaaa ggtggcgtgg ctgtcctcca gcacggtgtt tttcgccgcg
    12781 atggtcagct tgccgccctc ggtcatcgcg tcccgcgcat tgacggccaa gttgagcagc
    12841 gcgctttcga gctggttctg gtcgatttcg acccgccaca gatcgggcgg cagaacggcc
    12901 tccagcgtga tctgctcgcc cagcgtccgg tgcaggatct cgctcatggc gaagatcaac
    12961 tggtcgagtt cggtcgcctt gggatcgagc ggctgctggc gcgagaaggc cagcagccgc
    13021 gaggtcaggc tgccggcccg ctgggcgccc tgcat
//
LOCUS       DATGEM010000083        18063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_656129, whole genome shotgun sequence.
ACCESSION   DATGEM010000083 DATGEM010000000
VERSION     DATGEM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656129"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="VKQ27_02715"
     CDS             <1..103
                     /locus_tag="VKQ27_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLY87875.1"
                     /translation="DQVRGMDIQFVTTAKTDAEAKALLKAFDLPFAN"
     gene            114..419
                     /gene="rpsN"
                     /locus_tag="VKQ27_02720"
     CDS             114..419
                     /gene="rpsN"
                     /locus_tag="VKQ27_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HLY87876.1"
                     /translation="MAKTSQVNRNGMREYKVKRDKSKRSALKAIVMDRTLPVEDRFNA
                     TLKLAQLPRNGSAVRVRLRCELTGRSRGNYRKFKLCRIKLRDLANQGQIPGMVKASW"
     gene            434..832
                     /gene="rpsH"
                     /locus_tag="VKQ27_02725"
     CDS             434..832
                     /gene="rpsH"
                     /locus_tag="VKQ27_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLY87877.1"
                     /translation="MAVSDPLGDMLTRIRNAQRARHSMCMAPASRLRANVLDVLKREG
                     FIRGFAAEELRPGVAQLRIELKYNEGEPVIKEITRISKPGRRVYSKIKELPRVYAGLG
                     ISILSTPRGVMSDAEARAANVGGEVLCRVF"
     gene            844..1377
                     /gene="rplF"
                     /locus_tag="VKQ27_02730"
     CDS             844..1377
                     /gene="rplF"
                     /locus_tag="VKQ27_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLY87878.1"
                     /translation="MSRVGKYPVEIPQGVQVAIAGRLLTAKGKLGELKLELTDLIEAK
                     VDGNHVSVEPRSQETRSRMMWGTTRALIACMVKGVSAGYQKSMEITGTGYRAAVAGTN
                     LELALGYSHPIVYPIPPGIKITCERPTAIKVEGVDKRLVGQVAAEIRAFRGPEPYKGK
                     GIRYSDEQIRRKEGKKK"
     gene            1374..1736
                     /gene="rplR"
                     /locus_tag="VKQ27_02735"
     CDS             1374..1736
                     /gene="rplR"
                     /locus_tag="VKQ27_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLY87879.1"
                     /translation="MSTLQDLRTRRKERLRFQLRHKSGGRPRLSVFRSGKHIYAQVID
                     DGVGRTLAAASSLDKSLREGLKTGADKSAASAVGKLVAERALAAGVKEVVFDRGSYLY
                     HGRVKALADAAREGGLAF"
     gene            1748..2356
                     /gene="rpsE"
                     /locus_tag="VKQ27_02740"
     CDS             1748..2356
                     /gene="rpsE"
                     /locus_tag="VKQ27_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007282738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HLY87880.1"
                     /translation="MAREPREGGGRRRDREQEREDDGLTDKLVTINRVAKVVKGGRRF
                     AFAALVVVGDQKGRVGYGAGKAREVPEAIRKATERAKRSMIRVPMKEGRTLHHDIEGR
                     YGAGKVVLRSAVAGTGIIAGGPLRAVFETLGMGDVVAKSLGSRNPHNMVKATFAALER
                     CASPRSVANRRGKKVSELFRRDQPQAPDRGPGQAPAQETASA"
     gene            2349..2531
                     /gene="rpmD"
                     /locus_tag="VKQ27_02745"
     CDS             2349..2531
                     /gene="rpmD"
                     /locus_tag="VKQ27_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HLY87881.1"
                     /translation="MPKVRVTQTASPLGRKPGQKETLIGLGLNKMRRTRELEDTPAVR
                     GMIRKVAHLVKVEDLR"
     gene            2528..3022
                     /gene="rplO"
                     /locus_tag="VKQ27_02750"
     CDS             2528..3022
                     /gene="rplO"
                     /locus_tag="VKQ27_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HLY87882.1"
                     /translation="MRLNELRDNDGARLKSKRLGRGIGSGKGKTSGKGVKGQLARTGV
                     SINGFEGGQMPIFRRLPKRGFTNLFRKEYAPVNLGALEAAIAAGKLDAGAPIDEAALR
                     AAGLVRGKLDGVRLLANGEITRAVTITVSGASAAAIEAVQKAGGSVTTTVAKREAKVA
                     EAGA"
     gene            3187..4524
                     /gene="secY"
                     /locus_tag="VKQ27_02755"
     CDS             3187..4524
                     /gene="secY"
                     /locus_tag="VKQ27_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HLY87883.1"
                     /translation="MASAAEQLAANLSLGVFSKATELKKRIWFTLGALIVYRIGTYIP
                     IPGVDASVMTEMLNQHGGGILGMFDNFTGGALRRMTVFALNIMPYISASIIIQLMTAA
                     IPSLEALKKEGETGRKKLNQYTRYLTVFIAMFQSYGIAVGLESMHGTFGPAVLAPGYF
                     FRLSCVITLVGGTIFLMWLGEQITARGVGNGTSLIIMAGIVAGLPTALASLLDLGRTG
                     ALSPVFIIAFIVLAVFVIAFIVFMERAQRRIPIQYPKRQVGTRMFGGDSTHMPLKINT
                     SGVIPPIFASSILLIPATVAGFSQGGPAWLQFISNNLGPGAPLHMALYAAMIIFFSYF
                     YTAVVFNPEETADNLKKYGGFIPGIRPGSATAEYFDHVLTRLTTVGGLYLTAVCLLPE
                     ILISDYGLPFYFGGTSILIVVSVTMDTVTQIQSHLLAHQYEGLIRKSRQRGRR"
     gene            4589..5248
                     /locus_tag="VKQ27_02760"
     CDS             4589..5248
                     /locus_tag="VKQ27_02760"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLY87884.1"
                     /translation="MRLILLGPPGAGKGTQAKRLQDRYGIAQISTGDMLRAEVAAGTP
                     IGREAKAVMEAGQLVSDDIIVRMLGSRVEQPDAANGFILDGFPRTVPQAEALDRLLAE
                     KRMKLDAVIELQVDDAALVERIAGRFTCAKCGAGYHDKFKPTAVPGVCDVCGSHEFSR
                     RADDNRETVVARLEAYHRQTAPILPHYERQGLLHSVDGMAEIDEVTRQLLAVLDRTIA
                     R"
     gene            5317..6090
                     /locus_tag="VKQ27_02765"
     CDS             5317..6090
                     /locus_tag="VKQ27_02765"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HLY87885.1"
                     /translation="MIVFLYSLSDISVAALFGVVVAVVFAAAPLLRSRLFGPVSAANA
                     EIARTSMTAITGFTGAVLAFSLVQAQGNLRSVEKTVAAEAMQLGQVDRLLVGYGDVTA
                     GSIREAIRAYAESVVADEWPELSRHGTSGRTAELFREFTQRILALRPASARETVIYSD
                     LVRSVDQLAENRHDRLTATDLGLPPIYWEVIVSLMVLLVAFSAFVEPQRAIALGGLGA
                     GLALLITLVFIFDQPFLGDVSVTPAALVNVLQVIGGRVG"
     gene            6255..6632
                     /gene="rpsM"
                     /locus_tag="VKQ27_02770"
     CDS             6255..6632
                     /gene="rpsM"
                     /locus_tag="VKQ27_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLY87886.1"
                     /translation="MARIAGVNIPTNKRVTIGLRYIFGIGPSKAAEICEKLSIPNDRR
                     VNQLSDDEVLRIRELIDRDYRVEGDLRREVAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGKAVAIAGKKKVTK"
     gene            6679..7077
                     /gene="rpsK"
                     /locus_tag="VKQ27_02775"
     CDS             6679..7077
                     /gene="rpsK"
                     /locus_tag="VKQ27_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLY87887.1"
                     /translation="MARAATSTTRPRRKERKNITSGVAHVAATFNNTVITITDAQGNA
                     IAWSSSGSQGFKGSRKSTPYAAQVAAEDAGRKAREHGMEMLEIEVSGPGSGRESALRA
                     LQAVGFAISAIRDMTPIPHNGCRPRKRRRV"
     gene            7170..8201
                     /locus_tag="VKQ27_02780"
     CDS             7170..8201
                     /locus_tag="VKQ27_02780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLY87888.1"
                     /translation="MKQSAVIQRNWQSLIKPEKLEVEPGADPAKMATVVAEPLERGFG
                     MTLGNAIRRILLSSLQGAAVTAVQIDGVLHEFSSIPGVREDVTDIVLNIKQLALRMHG
                     EGPKRMTLTATGPGEVRAGQIQAGHDIEIMNPELVLCTLDDGVKLGMEFTVNLGKGYQ
                     PAAVNRPEDAPIGLIPVDSIYSPVRRVSYRVEPTRVGQVTDYDKLLLTVETNGAVTPE
                     DSVALAARILQDQLQLFINFEEPQQIRAEEPEDNLPFNRNLLRKVDELELSVRSANCL
                     KNDNIVYIGDLVQKSEQEMLRTPNFGRKSLNEIKEVLGSMGLSLGMSVPGWPPENIED
                     LAKRMEEPF"
     gene            8282..8707
                     /gene="rplQ"
                     /locus_tag="VKQ27_02785"
     CDS             8282..8707
                     /gene="rplQ"
                     /locus_tag="VKQ27_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLY87889.1"
                     /translation="MRHGVAGRKLGVTSTHRQAMFRNLAVALLKHEQITTTLAKAKEL
                     RPVAEKLVTLGKKGGLANQRLAHAQLRDDKIVAKLFGPLAERYRARAGGYCRVLKAGV
                     RYGDAADMAVIELVERDVLAKGQDSGPKQEVAAEEAEAA"
     gene            complement(8756..10027)
                     /locus_tag="VKQ27_02790"
     CDS             complement(8756..10027)
                     /locus_tag="VKQ27_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY87890.1"
                     /translation="MKDEPYWWEHARPTALETPALPAHVDVAVIGSGYTGLSAALTVA
                     RAGRSVIVLEADKPGVGASSRNGGMLGHALKPSLSTLSRRYGEKTAKALLTEAREAYD
                     FLGRFIAAEGIECDYVETGAFTGIVKPAQYESLARETEQLSRTIGYEAHMVPKSEVRD
                     EIGTDIYCGGRVTPHRAGLHPARYHAGLIERVRQAGGAVHGNCPVTMIQQSGSGFTVT
                     MPGASVSARNVIVATNGYTGDVTQSLRRRVIPVTSYMIATAPLSSNLMATLMPKGRML
                     SDSNRLLCYFRPSPDNTRVLFGGRPAYTDIGPQRAAERLSRYMTRIFPELSDVEVTHS
                     WFGYIAYTFDRLPHVGERDGMHYAMGYCGSGVVMSTWLGRKAALRLLGQPEGKSAFAD
                     IKHPTSPVYHGKPWFLPLVQAWYQGADMLGR"
     gene            10064..11221
                     /locus_tag="VKQ27_02795"
     CDS             10064..11221
                     /locus_tag="VKQ27_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY87891.1"
                     /translation="MQLGKIARYERGYARLSDGALVKFIGVEKSYDGRSLAVRDLTLD
                     VGRGEFLTLLGPSGSGKTTTLSMLAGFERPTRGEILLAGRPVDRLPPYERNIGMVFQN
                     YALFPHMTVGENVAFPLSVRRVSRAEVADRVKRALAMVRLEAFADRRPAQLSGGQQQR
                     IALARALVFEPSLVLMDEPLGALDKKLREQLQIEIKLLHERLGLTVVYVTHDQSEALT
                     MSDRIAIFHDGRIVQQGTPDVLYTHPCDAFVAGFIGENNMLAGTVAAIADGQVRVALA
                     GGPDVMATRVGELDHGSPVLVAVRPEVVRVSAGPAGGDNACRAVVTSQIYFGDHQRLL
                     TDIGTGHRVTVKVPAGVVTAAGDAIFLCWNTEDCRAFPASADVEASLEQET"
     gene            11290..12294
                     /locus_tag="VKQ27_02800"
     CDS             11290..12294
                     /locus_tag="VKQ27_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HLY87892.1"
                     /translation="MAAASADAQQPQLTIASAGGAWQQAQHNAWYAPFAQKMGIKFNE
                     EETSAGMARVEAMVKAGNVTDDVVTVDTAQVLAACDSGVLVRLDWSKIAPRDGFLPGA
                     ARDCGMGLDVYGDILAYNTDVVKSDPPTSVLALFDTKRWPGKRGMQKIAPNNLEWALE
                     ADGVPPDQVYKVLATPQGVDRAFAKLDTIKKDIVWWQAGAQPPQLLASHEVVMTTAWN
                     GRIQNPIDHDHAPFKIVWNNEIIEYDMISIPKGSPNLELAYKYLAYVAEPENNAKLGQ
                     FIPYGPVVKDAVKFVPADTLPKLPTAPDHMANALPIDMEFWADYGQDLTKRFNDWLAK
                     "
     gene            12299..13537
                     /locus_tag="VKQ27_02805"
     CDS             12299..13537
                     /locus_tag="VKQ27_02805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87893.1"
                     /translation="MSASAALTAADPALAAAVRRTTRGQRVRALLLVAPLLALLLLSF
                     CLPIGILLSRAVYDPATHDALPRTAAALAASPDQGVPNNAVFAALGDDLLAAKQNDSL
                     YNLARDLNKLLPAARSHVLRTARDLSPDKPVTRAALVAADPFWGESETWFAIRSGVRA
                     FTANYLLAAVDLRWTRDGSLGAVPSGQAIYRAIFARTFGIAAVVTVLTLILGFPIAWL
                     LAHVPGRLAARLIMLVLLPFWTSILVRTAAWTVLLQKFGILNDVLLALHVTDDRFDLM
                     YTRTGLIIAMVHIQLPFTLLPIYSIMRTISASQMRAAYSLGAHPFYAFRRVYLPQVMP
                     GVMAGCLLTFILCLGYFITPALVGGPADQLIANFISNDINVDLNWQMAGALSCILLLL
                     TLGLFAVFARMFGIDRMKLV"
     gene            13540..14343
                     /locus_tag="VKQ27_02810"
     CDS             13540..14343
                     /locus_tag="VKQ27_02810"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY87894.1"
                     /translation="MLLSPYATLGDRVRITLLVMFCALVLAFLVLPIFVPVPLSVNSE
                     AFFTYPLKGFSWRWYRDVLGSGQWRDAIWHSMVIAIGVTVLATSLGTLAAVGLSSPSM
                     PARRSIMAVLISPLIVPVVITAVGAYLFYAPIGLANSFLGIIIAQTTVASPFVVVTVG
                     ASLANFDRTLMRAAAISGARPLVAFQRVMLPSILPGVLSGAAFAFVASFDELVIALFL
                     AGPAQRTLPMQMFVGLREHLDPTITAAATLMMSLSILLLVVADWLSRRR"
     gene            14553..15866
                     /locus_tag="VKQ27_02815"
     CDS             14553..15866
                     /locus_tag="VKQ27_02815"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439016.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HLY87895.1"
                     /translation="MRTDPFHLAWFLQGSSIQAWGASWTGNIADEWMSAEVLIDLARN
                     IERAGFDYMLLEDSIYVGENHHTGRDFFLKRGMSIPRQEPSIVATLMAAATSRLGIVP
                     TLSTFAYAPYLVARIVGTLDQVSAGRGGWNMVTGSSDLSAMNFGMDRLPPHDMRYDMA
                     EEFVEICKKLWGSWEPGCIVNDEENHILIDPSKVHTIDYQGRYYSSRGPLNSGPCPQG
                     QPVIAQAGGSERGKSFAAKWADTIVSHPKGVDAMKAYKADITRKLKEYGRDPSSCKVL
                     YLVGPLLGETKEDAEELLRRRNASAAQNVEYSLARLGWITNIDFSQMDLDAPVGELST
                     NGHQESLRQFLVKAGKRTLREAMIDYSTSGQSVDLVGTPDAVAGQMEEIMQEVGGDGF
                     LFSMPNVSRRSTAEICDGLIPALQKRGLVRKQYAHKQFRDNLLEF"
     gene            15928..17283
                     /locus_tag="VKQ27_02820"
     CDS             15928..17283
                     /locus_tag="VKQ27_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HLY87896.1"
                     /translation="MAARGDPSAEDLFGQLSEPGPEPLAGPRPLADRLRPRSLEEVVG
                     QDHLLGAEGTLTRMLARGSLASLILWGPPGCGKTTIARLLAAQSGLHFVQLSAVFSGV
                     ADLKRAFDEAARRRRTGQGTLLFVDEIHRFNRAQQDGFLPVVETGTITLIGATTENPS
                     FALNGALLSRCQVMVLRRLDDAALEQLLSRAEMEIGRSLPLDSEARATLRAMADGDGR
                     YVLNMAEQIFALPDDTAPMDPTELAALLARRAALYDKDREEHYNLISALHKSLRGSDP
                     DAALYWFARMLNGGEDPRYIARRLVRFAAEDIGMADPNALPHSLAGWETYERLGSPEG
                     ELAIAQVVVYLATAPKSNAIYTAFGAARASAKATGSLMPPAHILNAPTRLMKELGYGD
                     GYQYDHEAEEAFSGQNYFPDGMAREQFYRPRDRGFEREISKRLDYWSKLRAARSDRSD
                     G"
     gene            17276..>18063
                     /locus_tag="VKQ27_02825"
     CDS             17276..>18063
                     /locus_tag="VKQ27_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY87897.1"
                     /translation="MDNEVFSFGSFQLIPVQRVLLADGKPIRLGSRAFDLLTALVEQA
                     GELIPRDKLMARAWPDTVVEEGSLRVHLAALRKVLGGGAGDSFIVNVPGRGYQFVAPV
                     QRGQTGVVARSPAVPLGNLPAPLSRIIGRDAAIATGTRQLAQHRCLTIVGPGGIGKTT
                     MATAVASTAAASYPDGVWFVGFASLPEPDLVPSAIAAALAISLPVENPVSGLTAWLRD
                     KTALIVLDGCEHVIAAAAEVAEAILHASPQVHILATSREPLRIAG"
BASE COUNT         3153 a         5263 c         6300 g         3347 t
ORIGIN      
        1 cgaccaggtg cgcggcatgg acattcagtt tgtcaccacc gcgaagacgg acgcggaagc
       61 gaaggcgctg ctgaaggcct tcgatctgcc gttcgccaac tgaggattta gggatggcca
      121 agacctcgca ggtcaaccgt aacggcatgc gtgaatacaa ggtgaagcgc gacaagtcga
      181 agcgctccgc gctgaaggcg atcgtgatgg atcgcacgct gccggtggag gatcgcttca
      241 acgccacgct gaagctggcg cagctgccgc gcaacgggtc agccgtgcgc gtgcgtttgc
      301 gctgcgagct gacggggcgg tcgcggggca actaccgcaa gttcaagctg tgccgcatca
      361 agctgcgcga tctggccaac cagggacaga tccccggcat ggtcaaggcg agctggtaag
      421 agggaacggg tagatggcag tttccgatcc cctgggtgat atgctgacac gcatccgcaa
      481 tgcgcagcgc gcccggcatt cgatgtgcat ggcgccggcg tccaggctgc gcgcgaatgt
      541 gctggacgtg ctgaagcgcg agggcttcat ccgcggcttc gcggccgagg agctgcgtcc
      601 aggcgtggca cagcttcgca tcgagctgaa gtacaatgag ggcgagccgg tcatcaagga
      661 gatcacgcgg atctccaagc cggggcggcg cgtctattcg aagatcaagg agctgccgcg
      721 cgtgtatgcg gggttgggca tctcgatcct gtccacgccg cgcggcgtga tgagcgatgc
      781 ggaagcacgc gctgccaatg tcggcggcga agtgctctgc cgcgtgttct gacggagagc
      841 gccatgtcgc gggtgggcaa atatccggtc gagattccgc agggcgtgca ggtggctatt
      901 gccggacgcc tgctgaccgc caagggcaag ctcggcgagc tgaagctgga gctgaccgat
      961 ctgatcgagg ccaaggtcga cggcaaccac gtgtcggtgg agcctcggtc gcaggagacg
     1021 cggtcgcgca tgatgtgggg aaccacgcgg gcgctgattg cgtgcatggt gaagggggta
     1081 tccgccggct accagaagtc gatggagatc accggcacgg gatatcgtgc ggcggtggca
     1141 ggcacgaacc tggagctggc gctggggtat tcgcatccga tcgtctatcc cattccgccg
     1201 ggcataaaga tcacctgcga gcggccgacg gcgatcaaag tcgagggcgt ggacaagcgg
     1261 ctggtgggtc aggtggcggc ggagatcagg gcgttccggg ggccggagcc ttacaagggc
     1321 aagggcatcc gctactcgga cgagcagatc cggcgcaagg aaggcaagaa gaaatgagca
     1381 cgcttcagga tctgcggacg cgacggaagg aacggctgcg cttccagttg aggcataagt
     1441 cgggcgggcg gcctcggctg tcggtgttcc ggtcgggcaa gcatatctat gcgcaggtca
     1501 tcgacgatgg cgtggggcgc acgctggccg cggcgtcctc gctggacaag tcgctgcgcg
     1561 aagggctgaa gaccggagct gacaagtccg cggcgagcgc ggtgggcaag ctggtggcgg
     1621 agcgcgcgct ggcggccggg gtcaaggaag tggtgttcga ccgcgggtcc tatctttacc
     1681 acggccgcgt caaggcgctg gccgatgctg cccgtgaggg cggcctggcg ttctgaggaa
     1741 ctgacaaatg gcacgtgaac caagggaagg cggcgggcgt cgccgcgatc gggagcagga
     1801 gcgtgaggac gacggcctca ccgacaagct cgtcacgatc aaccgcgtgg cgaaggtggt
     1861 gaagggcggc cgtcgatttg ctttcgcggc actggtcgtc gtgggcgacc agaaaggccg
     1921 cgtgggttat ggcgcgggca aggcgcgcga ggtgccggag gcgatccgca aggccacgga
     1981 acgtgccaag cgcagcatga tccgtgtgcc gatgaaggaa ggccgcacgc tgcaccacga
     2041 catcgagggc cgctacggcg ccggcaaggt cgtgctgcgc agtgcggtgg cgggcaccgg
     2101 catcatcgct ggtgggccgt tgcgtgcggt gttcgagacc ctgggcatgg gcgacgtggt
     2161 ggcgaagtcg ctgggcagcc ggaacccgca taacatggtg aaggcgacgt ttgcggcgct
     2221 ggagcggtgc gcttcgcctc ggtcggtggc gaaccggcgg gggaagaagg tgtcggagct
     2281 gttccggcgt gatcagccgc aggccccgga tcgtggtccg gggcaggcgc cagcgcagga
     2341 gacggccagt gcctaaggtg cgcgtgacgc agaccgcttc tccgctcggc cgcaagcccg
     2401 ggcagaagga gacgctgatc ggtttggggc tgaacaagat gcggcggacg cgcgagctgg
     2461 aggatacgcc ggcggtgcgc ggaatgatcc gcaaggtggc ccacctggtg aaggtggagg
     2521 atttgcgatg aggttgaacg aacttcgcga taacgatggc gctcggctga agtcgaagcg
     2581 gctggggcgc ggtatcgggt ccggcaaggg caagacctcg ggtaagggcg tgaagggcca
     2641 gctggcgcgc accggcgtgt cgataaacgg gttcgagggc gggcagatgc cgatcttccg
     2701 ccgcctgccg aagcgggggt tcacgaacct gttccgcaag gaatatgcgc cggtgaatct
     2761 gggtgcgctg gaggcggcga tcgcggcggg gaagctcgat gcgggtgcgc ctatcgatga
     2821 ggcggcgctg agggcggccg ggctggtccg gggcaagctg gatggcgtgc ggctgctggc
     2881 caatggcgag atcaccaggg cagtgacgat cacggtgtcc ggggcgtcgg ctgcggcgat
     2941 cgaggcggtg cagaaggctg gcgggtcggt caccacgact gtggcgaagc gggaggcgaa
     3001 ggtcgctgag gctggcgcct gacgggcgtc cggcggaggt gcgtcacgca cctcgctcat
     3061 ccctcgggtc gcgcccccgg gcaagctgct ccgcccccaa gggcgtagag gggttttaag
     3121 gggctggtaa cttgcactcg ccaggcggcg gaccgacaat agaacgcgac tgacgaggga
     3181 cgtttcatgg cctccgctgc cgagcaactt gcagcaaatc tcagcctcgg cgtcttctcg
     3241 aaggcgaccg agctcaagaa gcgcatatgg ttcacgctcg gcgcgctgat cgtctatcgg
     3301 atcggcacct acattccgat tcccggcgtc gatgccagcg tgatgaccga gatgctgaac
     3361 cagcatggcg gcggcatcct tggcatgttc gacaatttca ccggcggcgc gctgcggcgc
     3421 atgacggtgt ttgcgctgaa catcatgccc tacatcagcg ccagcatcat catccagttg
     3481 atgaccgcgg cgatcccgtc gctggaagcg ctgaagaagg aaggcgagac ggggcgcaag
     3541 aagctcaacc agtacacccg atatctgacg gtgttcatcg cgatgttcca gtcttacggg
     3601 atcgcggtgg ggctggagag catgcacggg acattcgggc ctgcggtgtt ggcgcctgga
     3661 tatttcttcc ggctctcgtg cgtgatcacg ctggtgggcg gcacgatctt cctgatgtgg
     3721 ctgggtgagc agatcaccgc gcgcggggtg ggcaacggca cgtcgctgat catcatggcg
     3781 ggcatcgtcg ccggtttgcc gacggcgctg gcatcgctgc tggatttggg gcgaaccggg
     3841 gcgctgtcgc cggtcttcat catcgcattc atcgtgctgg cggtgttcgt gatcgcgttc
     3901 atcgtgttca tggaacgcgc gcagcgacga atcccgatcc agtatccgaa gcggcaggtg
     3961 ggcacgcgga tgttcggcgg cgattcgacg cacatgccgc tgaagatcaa cacgtcgggg
     4021 gtgatcccac cgatctttgc cagctctatc ctgctgattc cggcgactgt ggcggggttc
     4081 tcgcagggag ggccggcgtg gctgcagttc atctcgaaca atctggggcc gggtgcgccg
     4141 ctgcatatgg cgctgtatgc ggccatgatc atcttcttct cgtatttcta cacggcggtc
     4201 gtgttcaatc cggaggagac ggcggacaac ctgaagaaat acggcgggtt cattccgggg
     4261 atccggccgg ggagtgcgac agccgaatac ttcgaccatg tgctgacgcg gctgaccacg
     4321 gtgggcggtc tgtatctgac agcggtgtgc ctgctgcccg agatcttaat cagtgattat
     4381 ggattgccgt tctatttcgg cggcacgtcg atcctgatcg tggtgtcggt gacgatggat
     4441 acggtgacgc agatccagtc gcacttgctg gcgcaccagt atgaggggct gatccgcaag
     4501 tcgcgacaac gcgggcgaag atgatgctgg ctgggttacg cccggccatg accggggggg
     4561 accgggtcta cgcccgggat aacatatgat gcggctgatc ctgctgggtc cacccggtgc
     4621 cggaaagggc acccaggcga agcgcctgca ggaccgctac ggcatcgcgc agatctccac
     4681 tggtgacatg cttcgcgccg aggtcgccgc cggtacgccg atcgggcgtg aggcaaaggc
     4741 ggtgatggag gccgggcagt tggtgtcgga tgacatcatc gtccgcatgc tgggcagccg
     4801 cgtggagcaa ccggatgcgg ccaatggctt catcctggat ggatttccac gcaccgtacc
     4861 ccaggccgag gcgctggacc ggctgctcgc cgagaagcgc atgaagctgg atgcggtgat
     4921 cgagctgcag gtggatgatg cggcgctggt ggaacggatc gccgggcggt tcacctgtgc
     4981 caagtgcggc gccggctacc acgacaagtt caagccgaca gcggttcccg gggtgtgcga
     5041 cgtgtgcgga tcgcacgaat tcagccgccg ggctgacgac aaccgtgaga cggtggtggc
     5101 gcggctggag gcgtatcacc ggcagacagc gccgatcctg ccgcactatg agcggcaagg
     5161 gctgctgcat tccgtcgatg gcatggcgga gatcgacgag gtgacgcggc aattgcttgc
     5221 ggtgctggat cggacgatcg cgcgatagag cccctgctaa gggtggatta agtccggcct
     5281 gttagaactt cccggtggct ggaagtttga ccggggatga tcgtattcct ctacagtctc
     5341 tcggacatca gcgtcgccgc gttgttcggc gtcgtggtcg cggttgtatt cgccgccgcg
     5401 ccgctgcttc ggtcgcgttt gttcggcccg gtgagtgcgg ccaatgccga gatcgcccgc
     5461 acgagcatga ccgcgatcac tggctttacc ggcgccgtgc tggcgttctc cttggtgcag
     5521 gcgcagggca atctgcgcag cgtcgagaag accgtggcgg ccgaggcgat gcagctcggc
     5581 caggtggaca ggcttcttgt cggctacggc gatgtgacgg ccgggtcgat ccgcgaggcg
     5641 atacgcgcct atgctgaatc cgtggtggcg gacgagtggc ccgaactctc ccggcatggg
     5701 acgagcgggc ggaccgcgga gctgttccgc gagtttaccc agaggatcct ggcgcttcgg
     5761 ccggcatctg cgcgtgagac agtgatctac agcgacctgg tgaggagcgt cgatcagctg
     5821 gcggaaaaca ggcacgatcg gctgaccgcg accgatctcg gactgccgcc gatctactgg
     5881 gaggtgatcg tctccctgat ggtgttgctg gtggccttct cggcattcgt ggagccgcag
     5941 cgggcgattg cgctgggagg cctgggagcg ggtcttgcgc tgctgattac gctggttttc
     6001 attttcgacc agccgttcct gggggatgtt tcggtcacgc cggccgcgct ggtgaatgtg
     6061 ctgcaggtca ttggcggtcg cgtaggctga acttgcgggt ttgcaaccgg cttgcaatga
     6121 tgacggccac gtccggccgc acgcgaattt gatccgatca cattgactcg cgagccgccg
     6181 gcactatatt ccgccgctcc ggcgcaaccc actgggctgc gcccctatag gcatttccct
     6241 aggagagtgg cggcgtggca cgtattgccg gcgtgaatat cccgaccaac aagcgggtga
     6301 cgatcggact gcgctatatc tttggcatcg ggccgtccaa ggccgccgag atctgcgaga
     6361 agctcagcat cccaaatgac cgccgggtca accagctctc cgacgacgag gtgctgcgca
     6421 tccgcgaact gatcgaccgt gattaccgcg tcgaaggcga tctgcggcgt gaagtcgcca
     6481 tgaacatcaa gcggctgatg gacttgggct gctatcgcgg cctgcgccat cgtcgcggcc
     6541 tgccggtccg cggccagcgc acccatacca acgcccgcac ccgcaagggc aaggcggtgg
     6601 cgatcgccgg caagaagaag gtcaccaagt agtccgactt cgcccatcgc ggatcagcag
     6661 cagcaggaat tttgcatcat ggcgagagcc gccacttcga ccacgcgccc ccgcaggaag
     6721 gagcgcaaga acatcacgtc cggtgtcgcc cacgtcgcgg cgacgttcaa caacaccgtc
     6781 atcaccatta ccgacgcgca gggcaacgcg atcgcgtggt cgtcgtccgg cagccagggt
     6841 ttcaagggca gccgcaagtc gacgccctat gccgctcagg tggccgccga ggatgcgggc
     6901 cgcaaggcgc gcgagcacgg catggaaatg cttgagatcg aggtcagtgg tccgggctcg
     6961 gggcgtgaga gcgcgctgcg ggcgctacag gccgtgggtt tcgcgatctc cgccattcgc
     7021 gacatgacac caattccgca caacggttgc cggcctcgca agcgtcggcg cgtgtaaggc
     7081 ggacttttcc ctgcgcggga ggaagcgcgc cgaacccatt gggtcgttgc aggaacccgg
     7141 cgaaagtccg ggtccgaaag gttgtataga tgaagcagag tgcagtcatc cagcggaact
     7201 ggcagtccct gatcaagccg gagaagctgg aagtagagcc gggcgccgat ccagcaaaga
     7261 tggcgacggt ggtggccgag ccgttggagc gcggcttcgg tatgacgctg ggcaatgcga
     7321 ttcgccgcat cctgctgtct tcgctgcagg gcgccgcggt taccgcggtg cagatcgatg
     7381 gcgtgctgca tgagttcagc agcatccccg gcgtgcgtga ggacgtgacg gacatcgtgc
     7441 tgaacatcaa gcagctcgcg ctgcggatgc acggcgaagg gccgaagcgg atgacgctga
     7501 cggcgacggg tcctggcgag gtgcgcgcgg gccagatcca ggcaggccat gacatcgaga
     7561 tcatgaaccc cgagctggtg ctgtgcacgc tggacgacgg cgtgaagctc ggcatggagt
     7621 tcacggtgaa cctgggcaag ggctaccagc ccgccgcggt gaaccggcct gaggatgcgc
     7681 cgatcggcct gatccctgtc gattcgatct attcgccggt gcggcgggtt tcctatcggg
     7741 tggagccgac acgcgtcggg caggtgaccg actacgacaa gctgctgctg accgtggaga
     7801 ccaacggcgc ggtgacgccg gaggattcgg tggcactcgc tgcccggatc ctgcaggacc
     7861 agttgcagct cttcatcaat ttcgaggagc cgcagcagat ccgtgccgag gagccggaag
     7921 acaacctgcc gttcaaccgc aacctgctgc gcaaggtgga cgagctggag ctgtcggtgc
     7981 ggtcggcgaa ctgcctgaag aacgacaaca tcgtctacat cggcgacctg gtgcagaagt
     8041 ccgagcagga gatgctgcgc acgccgaact tcgggcggaa gtcgctgaac gagatcaagg
     8101 aagtgcttgg ctcgatgggc ctgtcgctcg gcatgtcggt gcctggctgg ccgccggaga
     8161 acatcgagga tcttgccaag cggatggaag agccgttcta agccgtttga agttcgatcg
     8221 cgtgattcag gcctccggcg acgccgcctt cggtcatcac gctcaaaggg gatagcgtgt
     8281 catgcgtcac ggggtagccg gccggaagct cggcgttacg tccactcatc ggcaggcgat
     8341 gttccgcaac ctcgcggtcg cattgctgaa gcatgagcag atcaccacca cgctggcgaa
     8401 ggcgaaggag ctgcgcccgg tggctgagaa gctggtcacg ctgggcaaga agggcgggct
     8461 ggcgaaccag cggctggcgc acgcgcagct gcgcgacgac aagatcgtgg ccaagttgtt
     8521 cgggccgctg gcggagcggt atcgcgcgcg ggcgggcgga tattgccgcg tgctgaaggc
     8581 cggcgtgcgg tatggtgacg cggccgatat ggcggtgatc gagctggtcg agcgcgatgt
     8641 gctggcgaag ggccaggaca gcgggccgaa gcaagaggtc gcggccgagg aagccgaggc
     8701 ggcgtaactg ctccggcttt cggagttcag tggcttcggc ggaaacgacg aggccctatc
     8761 gccccagcat atcggcgccc tggtaccagg cctgcaccaa tggcaggaac cagggcttcc
     8821 cgtgatagac gggcgatgtt ggatgcttga tatcggcgaa agcgcttttc ccctccggct
     8881 gaccgagcag gcgtagcgcc gccttgcgcc cgagccaggt ggacatcacg acaccagatc
     8941 cgcagtagcc catggcgtag tgcatgccgt cgcgttcacc cacgtgcggc aaccgatcga
     9001 atgtgtaggc gatgtagccg aaccatgagt gcgtgacctc cacgtcggac agttcgggaa
     9061 atatccgggt catgtagcgt gacaggcgtt cggcggcacg ctgcggaccg atatcggtgt
     9121 aggccggcct gccaccgaag agcacgcgcg tgttgtcagg cgatgggcgg aaataacaca
     9181 gcaggcgatt gctgtcgctc agcatccggc ccttcggcat caaggttgcc atcaggttcg
     9241 acgatagcgg cgcagtcgcg atcatgtagc tggtcaccgg gatgacgcgc cggcggagcg
     9301 actgtgtcac gtcaccggta tagccatttg tcgctacaat cacgttgcgg gcgcttaccg
     9361 atgcgcctgg catggtgacg gtgaagccgc tgccactttg ttggatcatg gtgacggggc
     9421 agttgccgtg gaccgcgccc ccggcctggc gtacgcgctc gatcagcccg gcgtggtagc
     9481 gtgccggatg cagcccggcg cgatgcggcg tgaccctgcc accgcagtat atgtcggtgc
     9541 cgatctcgtc gcgcacttcg gacttcggca ccatgtgcgc ctcataaccg atcgtgcggg
     9601 agagttgctc ggtctcgcgt gcgagtgact cgtactgcgc aggtttgacg attccggtga
     9661 aggcgcccgt ctcgacatag tcgcactcga tgccttctgc agcgatgaag cggccgagga
     9721 aatcgtatgc ttcacgcgct tcggtgagta atgccttagc tgtcttctca ccataacgac
     9781 gtgagagtgt gctgagggac ggcttcagcg catgaccgag catgccgccg ttgcgcgagc
     9841 ttgcgccgac acccggctta tcggcctcca acacgatgac ggaacggcca gctcgagcga
     9901 cggtcagcgc tgccgagagt cccgtgtagc cggagccgat gaccgcaaca tcgacgtgag
     9961 ctggcaatgc tggtgtctcg agcgccgttg gccgcgcgtg ttcccaccaa tacggttcgt
    10021 ctttcatcgc cggctcctga cccgcggcac gttgcggcct tcgatgcagc ttggcaagat
    10081 tgcgagatac gagaggggat atgcgcgttt gagcgacggg gcgctcgtga agttcatcgg
    10141 ggtggagaag agctacgatg gccgctcact cgctgtccgt gacctgaccc tggatgtcgg
    10201 gcggggggaa ttcctcacgc tgcttggacc atcgggttcc ggcaagacga ccacgctgag
    10261 catgctcgct ggcttcgaac gccccacccg cggcgaaatc ctgctcgcgg ggcgcccggt
    10321 ggatcggctg ccgccctatg agcgcaatat cggcatggtg ttccaaaact acgcgctgtt
    10381 cccgcacatg acggtgggcg agaacgtcgc ctttccgctc agcgtccgcc gcgtgtcacg
    10441 cgccgaggtt gctgatcgcg tgaagcgtgc cttggcaatg gtgcggctgg aagcattcgc
    10501 tgatcgccgc cccgcgcagc tctcgggtgg acagcagcag cgcatcgccc tggcgcgtgc
    10561 gttggtgttt gagcctagcc tggtgctgat ggacgaaccg ctcggggcgc tcgacaagaa
    10621 gctgcgcgag cagttgcaga tcgagatcaa gctgctgcac gaacggctcg gtctgactgt
    10681 tgtctacgtg acgcacgatc aaagcgaggc gctgacaatg tccgaccgca tcgcgatttt
    10741 tcacgatggg cggatcgtgc agcagggcac gccggatgtg ctgtatacac atccgtgtga
    10801 cgctttcgtc gctgggttca tcggcgagaa caacatgttg gctggtactg tcgcagcgat
    10861 cgccgacggt caggttcggg ttgcgcttgc tggtggccct gatgtcatgg ccacgcgcgt
    10921 gggcgaattg gatcacggct caccggtgct tgtcgccgtg cggcccgagg tggtgcgcgt
    10981 gtcggctggt ccggcaggcg gcgacaacgc gtgccgtgcg gtggtgacga gccagatcta
    11041 ttttggtgac catcagcggc tgctgactga catcggcacc gggcacagag tgacagtcaa
    11101 ggttcctgcc ggtgtcgtaa ccgccgcggg agacgcgatt ttcctgtgct ggaataccga
    11161 ggattgccgt gcctttccgg cttccgccga cgttgaagcg tcactggaac aagaaacctg
    11221 aggggggttt gaacgatgtt gatgaaattg gtcagggcgt tggggattat cgctggggca
    11281 gctgggttca tggcagccgc ttcggccgat gcgcagcagc cgcagctgac gatcgcttcc
    11341 gcgggcgggg cgtggcagca ggcgcagcac aacgcctggt acgcaccttt cgcgcagaag
    11401 atgggcatca agttcaatga ggaagagacg tcggccggga tggctcgtgt cgaggcgatg
    11461 gtgaaggcgg gcaacgtgac cgacgacgtc gtcaccgtag atacggcgca ggtcctggcg
    11521 gcctgtgact cgggcgttct ggtccggctc gactggagca agatcgcgcc gcgcgacgga
    11581 tttctgcctg gcgcggcacg agactgcggc atgggcctcg atgtttacgg cgatatcctc
    11641 gcttacaata ccgacgtggt gaagagcgat ccgcccacat cggtgctcgc attgttcgac
    11701 accaagcgct ggcccggcaa gcgcggcatg cagaagattg cgcccaacaa cctggaatgg
    11761 gcgcttgagg cggacggcgt gccgcctgat caggtgtaca aagtgctggc aacgccacag
    11821 ggtgtggatc gtgcattcgc caaactcgac acgatcaaga aggacatcgt ctggtggcag
    11881 gccggcgcac aaccgccgca attgctcgcc tcgcatgagg tcgtgatgac cacagcatgg
    11941 aatggccgca tccagaatcc gatcgatcac gatcatgccc cgttcaagat cgtctggaac
    12001 aacgagatca tcgagtacga catgatctcg atccccaagg gatcgccgaa tctcgaactg
    12061 gcgtacaagt acctcgccta tgtggcggag ccagagaaca atgccaaact tgggcaattc
    12121 atcccatacg gtccggtggt gaaggatgcc gtcaagttcg tgccggcgga cacgctgccg
    12181 aagctgccga cggcgcctga tcacatggcg aacgccttgc ctatcgacat ggagttctgg
    12241 gcggactacg gccaggacct gaccaagcgg ttcaacgact ggctcgcgaa gtagcgcttt
    12301 gagcgcaagc gctgcgctga ccgcggcgga ccctgcgctc gctgcagcgg tgcggcgaac
    12361 aacccgcggt cagcgcgtgc gcgccttgtt actggtggcg ccactccttg cgttgctgct
    12421 actgagcttc tgtctgccga tcgggatctt gctgtctcgg gcggtgtatg acccggcgac
    12481 gcacgatgct ctgccgcgta cggcggcagc tctggcagct tcaccggacc agggcgtgcc
    12541 gaataacgct gtgtttgcgg cactgggtga cgatctgctg gcggcaaagc agaatgactc
    12601 gctctataac cttgccagag acctcaacaa gctgctgccg gccgcgcgca gccatgtgtt
    12661 gcgcacggcg cgcgacttat cgcccgacaa gccggtgacc cgcgccgcgc tggtggcagc
    12721 ggacccgttc tggggcgaga gcgaaacctg gttcgcgatc aggagcggcg tgcgggcctt
    12781 tacggcgaat tatctgttgg ccgctgtcga tctacgatgg acgcgcgatg gaagcctcgg
    12841 ggcggtgccg tcgggtcagg cgatctaccg cgcgatattc gcgcgcacgt tcgggatcgc
    12901 ggcggtcgtc actgtgttga ccctgatact cggcttcccg atcgcctggc tgcttgcgca
    12961 tgtacctggc cggctggcgg cacggctgat catgttggtg ctgctgccat tctggacatc
    13021 gatcctggtg cggacggcag catggacagt gctgttgcag aagttcggca tcctgaatga
    13081 cgtgctgctg gcgttgcacg tcaccgatga tcgcttcgat ctgatgtaca cgcggacggg
    13141 cctgatcatt gccatggtcc atatccaact gccgttcacg ctgctgccga tctacagcat
    13201 tatgcggacg atctctgcgt cacagatgcg ggcagcctat tcgcttggcg cgcatccatt
    13261 ttacgcgttc cggcgggtct atctgccgca ggtgatgcct ggagtcatgg cgggctgcct
    13321 gctgaccttc attctctgtc ttggctattt catcactccg gcgctggtgg gcggaccagc
    13381 cgaccagctg atcgcgaact tcatctcgaa cgatatcaat gtcgatctga actggcagat
    13441 ggccggagca ttgagctgca tcctgctgtt gttgacgctt ggattgtttg ccgtgtttgc
    13501 ccgcatgttc ggcatcgacc gcatgaaact cgtctgacca tgctgttgtc accatatgca
    13561 acgcttgggg atcgggtccg cattacgctg ctggtgatgt tctgcgcact cgtgctggcg
    13621 ttcctggtgc tgccgatatt cgttccggtg ccgctgtcgg tgaactcgga ggcgttcttc
    13681 acatatccgc tcaaggggtt ctcgtggcgc tggtatcgcg atgtgctcgg cagcgggcag
    13741 tggcgagacg cgatctggca cagcatggtt atcgccatcg gcgtgacggt gctggcaacc
    13801 agccttggga cgcttgcggc ggttggtctt tccagcccca gcatgccggc ccggcggagc
    13861 atcatggccg tgctgatatc gccactgatc gtgccggtcg tgatcaccgc tgttggcgcg
    13921 tatctgttct atgctccgat aggactggcc aattcgttcc tcggcatcat catcgcgcaa
    13981 acgacagtcg cttcgccgtt tgtggtcgtg accgttggtg caagcctcgc caattttgat
    14041 cgcacgctga tgcgggccgc ggcgatctcg ggcgcacggc cgctggtggc attccagcgc
    14101 gtgatgttgc cgtcgatttt gccgggcgtg ttgtccggcg ccgcgttcgc gtttgtcgct
    14161 tcgttcgacg aactggtcat tgcgttgttt ctcgccggac cagcgcagcg aacactgccg
    14221 atgcagatgt tcgtaggatt gcgcgaacac cttgatccga ccatcaccgc ggcggcgacg
    14281 ctgatgatgt cgctgtcgat cctgctgctg gttgtcgcgg attggctgag ccggcgccgc
    14341 tgaccgcggg cgctctgcgt ttgccgcaat tcagggcggt taccggctaa ctaatagtca
    14401 tagtgtcgcg cagagcggca aacgatgtcg ccagcattca aaaaatgacc tgaataccag
    14461 catctggccc tttccgatcg gaagttgctt ttgatacggt ctgccgactc tcgtgtcagt
    14521 cagacctgag acccggcaaa ggaatccgcc cgatgcgcac tgacccattc cacctcgcct
    14581 ggttcctcca aggctcttcg atccaggcct ggggtgcttc ctggacgggc aatatcgccg
    14641 acgagtggat gtctgctgaa gtgttgatcg acctcgcgcg caacatcgaa cgcgccgggt
    14701 tcgactacat gctgctggaa gactcgatct acgtcggcga gaaccaccac acggggcgcg
    14761 acttcttcct gaagcggggc atgtcgattc cgcgccagga gccctcgatc gtggcgacgc
    14821 tgatggcggc ggcgacgtca cgtctgggta tcgtgccgac gctttcgacg ttcgcttatg
    14881 cgccttatct cgttgcgcgg attgtcggca cactcgacca ggtttccgct gggcgcggcg
    14941 gctggaacat ggtgacgggc agttccgatc tctcggcgat gaacttcggc atggatcggc
    15001 tgccgccgca cgacatgcgc tatgacatgg ctgaggagtt cgtcgagatc tgcaagaagc
    15061 tgtggggatc gtgggagccg ggctgcattg tcaacgacga agagaaccac atcctgatcg
    15121 atccctcgaa ggtgcacacg atcgattacc aggggcgcta ctactcgagc cgtgggccgc
    15181 tgaactcggg gccgtgtccg caagggcagc cggtgatcgc gcaggccggc ggatcggaac
    15241 ggggcaagag ttttgcggcg aaatgggcgg acacgatcgt ctcgcatccg aaaggcgtgg
    15301 atgcgatgaa ggcctacaag gccgacatca cacggaagtt gaaggagtac gggcgcgatc
    15361 cgtcgtcgtg caaagtgctg tatctggtgg gaccgctgct gggtgagacg aaagaggacg
    15421 cggaggaact gctgcgccgg cgcaatgcct cggctgcgca gaacgtcgaa tattcgctgg
    15481 cgcggctggg gtggatcacg aacatcgatt tctcgcagat ggacctggac gcgccggtgg
    15541 gtgagctgag caccaacgga caccaggaga gcctgcgtca gttcctggtg aaggccggca
    15601 agcgcacgct acgcgaagcg atgatcgatt acagcacctc gggtcagtcg gtggacctgg
    15661 tcgggacgcc tgatgcggtg gcgggccaga tggaggaaat catgcaggag gtcggcgggg
    15721 acggcttcct gttctcgatg ccgaatgttt ctcggcggtc gacggcggag atctgcgacg
    15781 ggctgatccc ggcgctgcag aagcgtggac tggtgcgcaa gcaatatgcg cacaagcagt
    15841 tcagggacaa tctgctggag ttctgactgg gctgcggcca ggtcacggtg gctcgcttgg
    15901 gccggcggcc ttggctaaca tggctggatg gcagcgcgcg gagatccatc ggcggaagac
    15961 ctgttcggtc agctttccga gccgggacct gaaccgcttg ccggtccgcg tccgctggcc
    16021 gatcgactgc ggccgcgatc gctcgaggag gttgtcggcc aggaccacct gcttggagcg
    16081 gagggcacgc tcacgcggat gctggcgcgc ggttcgctgg cgtccctgat cctgtggggc
    16141 ccgccgggct gtgggaagac caccatcgcg cgcttgcttg ccgcgcagtc cggcctgcat
    16201 ttcgtgcagt tgtccgcggt gttttccggc gtagcggatt tgaagcgggc gttcgatgag
    16261 gcggcgcgga ggcggcgaac ggggcagggc acgctgctgt tcgtcgatga gatccaccgc
    16321 ttcaatcgtg cgcagcagga cgggtttttg cccgtcgtgg agaccggcac gatcacgctg
    16381 atcggtgcga ccacggagaa tccttcgttt gcgttgaacg gcgcgctgct gtcgcgctgc
    16441 caggtgatgg tgctgcgccg gcttgacgat gcggcactgg aacaactgct gtcgcgcgct
    16501 gagatggaga tcgggcgctc actgccactt gattccgagg cacgcgccac gctgcgcgca
    16561 atggcggatg gcgacggccg ctatgtgctg aacatggccg agcagatctt cgcgctgccg
    16621 gatgacacgg cgccgatgga tccaacggag cttgccgcgc tgctggcgcg ccgggccgcg
    16681 ctgtacgaca aggatcgcga ggagcactac aacctgatct ccgcgctgca caagtcgctg
    16741 cgcggttccg atccggatgc ggcgctctac tggttcgcgc ggatgctgaa tgggggcgag
    16801 gatccgcgct acatcgcgcg ccggctcgtc cggttcgccg ccgaggatat cggcatggcc
    16861 gatccgaatg cgttgccgca ctcgctcgcg ggatgggaga cgtatgagcg gcttggctcg
    16921 ccagaaggcg agctggcgat cgcgcaggtg gtggtctatc tggcgaccgc accgaaatcg
    16981 aacgcgatct acactgcctt cggcgccgcg cgcgcatcgg cgaaagcgac cggttcgctg
    17041 atgccgccgg cgcacatcct gaatgcaccg acccggttga tgaaggagct ggggtacggc
    17101 gacggctatc agtacgacca cgaggccgag gaagcgttct ctggccagaa ctactttcct
    17161 gacggcatgg cgcgcgaaca attttaccgt ccgcgtgacc gtggcttcga acgcgagatc
    17221 agcaagcgtc tcgactactg gtcgaagctg cgtgccgccc gctcagaccg gtctgatgga
    17281 taacgaggta ttctcgttcg gttcgtttca actgatacca gtccaaaggg tattgctggc
    17341 tgatgggaag ccgatacgtc tcggcagccg ggcctttgac cttctgacgg cgctggtcga
    17401 acaggcgggc gaactgatcc cgcgtgataa gctgatggca cgtgcgtggc ccgataccgt
    17461 ggtcgaggag ggatcgctgc gcgtgcacct cgcggcgttg cgcaaggtgc tcggcggcgg
    17521 cgcgggcgac agctttatcg tcaatgtccc gggtcgcggc tatcagttcg tggcacccgt
    17581 tcagcgaggc caaacgggtg ttgtggcgcg atcaccggca gtacccctcg gcaatctgcc
    17641 cgcgccactg tcccgcatca tcgggagaga cgccgcgata gcgaccggga cgcggcagct
    17701 ggcgcaacac aggtgcctga ccatcgtcgg ccctggaggt atcggcaaga ccacgatggc
    17761 aacggcggtc gcttcgaccg ctgccgcatc gtatccggac ggtgtttggt ttgtcgggtt
    17821 tgcttcgctg cccgaacccg atctggtgcc gagcgcaatc gccgccgcgc tggcgatttc
    17881 cttgcccgtt gagaatccgg tttccggtct gacggcatgg ctgcgcgaca agacggcgtt
    17941 gatcgtgctt gacggctgcg agcacgtgat tgccgccgcc gccgaggttg ccgaggcgat
    18001 cctgcatgct tcaccgcagg tgcacatcct cgccacgagc cgggagccgc tgcggatcgc
    18061 cgg
//
LOCUS       DATGEM010000084         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_655642, whole genome shotgun sequence.
ACCESSION   DATGEM010000084 DATGEM010000000
VERSION     DATGEM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655642"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1527)
                     /locus_tag="VKQ27_02830"
     CDS             complement(115..1527)
                     /locus_tag="VKQ27_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY87898.1"
                     /translation="MPIEALRSILPVAGLSAARAETVTFTGGTDPILPTPFRIGVAGA
                     ATLGAIGLAVSDLWEMRTGRSQSVAVDVRQATASLRSGHYMKLGDGEVSARRNSIMGV
                     YPTKDGRWSYLHCNFPNHRAAALSVLGVAEDRDAVSKAVLNWNAQDLEEAIIAAKGAG
                     GMVRSKAEWAQHPQSAAIASLPLLEIIRIGDAPPTPLPAGNRPLSGIRVLDLTRVLAG
                     PTCARSLAEHGADVLKITGKHLPNLGYQEFDTGHGKLSAHLDLREQRDVDVLRGLVRE
                     ADVFSQGYRPGTLGLRGLSPEELAAQRPGLVYVSLCAFSHAGPWASRRGFDTVVQTVS
                     GITTRQTEVIPNKTGAPEFYPVSAIDYCTGYLMAFGTMVALARRAREGGSWLVRISLA
                     QIGKWIVDLGEVTESAARDAPKEFAPEELERWSMVTDTPSGKLRHLRPTLQLSETQPY
                     WARPSVPLGYNKPEWPARSA"
     gene            1686..2501
                     /locus_tag="VKQ27_02835"
     CDS             1686..2501
                     /locus_tag="VKQ27_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY87899.1"
                     /translation="MQIGTMMRFTPGGLGEGGRLMERAAEYARRIEAAGFPAIWVGDS
                     LGRGNPTLDPLMELATLAAVTSKVELGIGVLQLPLRHPIELAHRVQSLQALSGNRLML
                     GVGSGSTKDDFDLLGYDYDKRFGTLRSSLRAMQAVWRGDSALSVWPTCKGGPPIMIGA
                     WRSHRWITLAAKEFQGWTPSGRYSSWDDLEAGMKVFREAGGTNAVLANTAVDLGNRPG
                     SAELAKVAEPALIGTPDEVRAKLKRMEDIGFESVMVVSHHNVLEDIERVRDML"
     gene            2501..2953
                     /gene="tsaA"
                     /locus_tag="VKQ27_02840"
     CDS             2501..2953
                     /gene="tsaA"
                     /locus_tag="VKQ27_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HLY87900.1"
                     /translation="MELRPIGVLHTPWRSIADCPRNGRQPNPAPLCTAVVLPAFVEGL
                     RSIEGFSHLIMVYWMDQVKTPELSFTPPFDTEVRGIFATRSPRRPNPIGLSVVAFEGL
                     EGGDTLKVRYLDCIDGTPLLDIKPYLKSTDCEPDSSMGWLEQHATRRR"
     gene            complement(2958..4130)
                     /locus_tag="VKQ27_02845"
     CDS             complement(2958..4130)
                     /locus_tag="VKQ27_02845"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY87901.1"
                     /translation="MLPLAGVRILAVEQYGAGPFGSMQLADMGAEVIKIENPAEGGDV
                     SRSVGPYFLGEHDSHFFQAFNRNKRSITLNLRTPEGQQVFRDLVATADGVLDNLRGDQ
                     PPRLGVTYDQLKQANPRIVCAHLSAYGREGSRAAWPGYDYLMQAEAGYLSLTGEPDQP
                     PARFGLSIVDLMAGVYAATALLGGIVSARATGVGRDVDVSLFDTALANVNYLAAWALN
                     GGHVQGREPRGAHPSLTPSQLYRTADGWIFIMCNKQKFWPVLCQKIDHAEWSDDPRFA
                     TFKTRLKNRAELTRLLDAALSMRGTEAWLAHFAGAVPAAPVYDVAQALGSEFVTHEER
                     VWSYDGFRMVAPAFRLPGEAMPRERAPELGADTETLLQEIGYDAGKISHLRKMGAI"
     gene            complement(4130..>4740)
                     /locus_tag="VKQ27_02850"
     CDS             complement(4130..>4740)
                     /locus_tag="VKQ27_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87902.1"
                     /translation="SMLLAEKGPGFTVSRKLEKLGYKGIDSAELVFQDCRVPADRLIG
                     GVEGKGLQHALSGLELGRINVAARGVGIAQAALDESLRYAQLRKTFGQPIAQHQAIQL
                     KLADMATRTEASRLLVRRAAEMYDRGDRCDLEAGMAKLFATETAMENAAEAMRIHGGY
                     GYSTEFPVERLYRDAPLLVIGEGTNELQRIIIARQLVARNPV"
BASE COUNT          815 a         1552 c         1533 g          840 t
ORIGIN      
        1 ttgccttcca tgaagcgctg gttggcaccg tgcataatca tcctctcgat tggttttgcc
       61 gccagtataa tagcgcagcc agcggcaaag cacgtcaaat cgtatacagg atgatcaggc
      121 tgagcgcgct ggccactccg gcttgttgta gcccagcggc accgacggcc gtgcccagta
      181 tggctgcgtc tcagacaact gcagcgtcgg ccgcagatgc cgcagcttgc ccgatggcgt
      241 atcggtcacc atcgaccagc gctccagctc ttctggcgcg aactccttcg gtgcatcgcg
      301 ggcggcgctc tccgtcacct cgccgagatc gacgatccac ttgccgatct gcgccagaga
      361 gatgcgcacc agccagctgc cgccctcgcg cgcgcgccgt gccagcgcca ccatcgtgcc
      421 gaacgccatc agatagccgg tgcagtagtc gatcgccgac accggataga attccggcgc
      481 gccggtcttg ttcgggatca cctcggtctg acgcgtggtg atgccgctga ccgtctgcac
      541 cacggtatcg aagccacgcc gtgacgccca cggtccggca tggctgaacg cgcacagcga
      601 tacatacacc aggccaggac gctgtgcggc gagttcctct ggcgacagcc cgcgcagacc
      661 cagcgtgccg ggtcgatacc cctgcgagaa cacatccgcc tcgcgcacca gtccgcgcag
      721 cacatcgaca tcgcgctgct cgcgcagatc aagatgcgcc gacagcttgc cgtgcccggt
      781 atcgaattcc tggtagccaa ggttcggcag atgcttcccg gtgatcttca gcacatccgc
      841 cccatgctcc gcgaggctgc gcgcgcaggt tggtcccgcg agcacgcgcg tcaggtccag
      901 tacgcggatg ccggacagcg gccggttgcc ggcaggcagc ggtgtcggag gcgcgtcgcc
      961 gatgcggatg atttcgagca atggcaacga tgcgatcgct gcggactgcg gatgctgcgc
     1021 ccattccgcc ttcgagcgca ccatcccgcc ggcacccttc gcggcgatga tcgcctcttc
     1081 caggtcctgc gcgttccagt tcagcaccgc tttcgagact gcgtcgcgat cttccgccac
     1141 gcccagcacg ctcagcgcgg ccgcgcgatg attggggaaa ttgcagtgca gatagctcca
     1201 gcggccatcc ttcgtcggat acacgcccat gatggagttg cggcgcgcgg atacttcgcc
     1261 gtcaccaagc ttcatgtagt ggccactgcg cagcgatgcg gtcgcctgcc gcacatcgac
     1321 cgcaaccgat tgcgatcggc cggtgcgcat ctcccacagg tcggagactg ccagaccgat
     1381 cgcgccgagc gtcgccgcac cggccacacc gatacggaag ggcgtcggca ggatcggatc
     1441 ggtgccgccg gtgaatgtca ctgtttcggc gcgcgcggcc gaaagaccgg caaccggcag
     1501 gatcgagcgc agggcttcta ttggcatgat cgagacctcc ccatggcgtc acggcccagc
     1561 atggcgcgcg gcgccgatgt attgcaaatt gcgcgggtca cgacgtgacg gatggctgga
     1621 tcaagtccgg tcatgacatg agaagctcgg gctcgcgccc gagatcaaac aagaggcagg
     1681 tggtcatgca aataggcacg atgatgcggt tcacgcccgg aggactgggc gaaggcgggc
     1741 ggctgatgga acgcgccgcc gaatacgcgc gccgcatcga ggccgcgggc tttcctgcca
     1801 tctgggtcgg cgattcgctt ggccgcggca atccgacgct cgatccgctg atggaactgg
     1861 cgacgctcgc cgccgtcacc agcaaggtgg aacttggtat cggcgtgctg cagctcccgt
     1921 tgcgtcaccc aatcgagctg gcccatcggg tgcagtcgct gcaggcgctg agtggcaacc
     1981 ggctgatgct tggcgtcggc agcgggtcca ccaaggacga cttcgatctg ctcggctatg
     2041 attacgacaa gcggttcggc acgttgcggt cgtcgctgcg ggcgatgcag gcggtgtggc
     2101 gtggcgactc ggcgctgtcg gtctggccaa cctgcaaggg cgggccgccg atcatgatcg
     2161 gcgcatggcg cagccatcgc tggatcacac tggctgcgaa ggaattccag gggtggacgc
     2221 catcgggacg ttacagcagc tgggatgatc tggaagccgg catgaaggtg ttccgcgagg
     2281 ccggcggcac caatgccgtg ctggcgaaca ccgcggtcga tctgggcaac cgtcctggtt
     2341 cggcggagct tgcgaaggtt gccgaacccg cgttgatcgg cacgccggac gaggtccgcg
     2401 caaagctgaa gcggatggag gacatcgggt tcgagtccgt gatggtggtc tcgcatcaca
     2461 acgtgctgga ggacatcgag cgcgtccgcg acatgctgtg atggaactgc gtccgatcgg
     2521 cgtgctgcac actccgtggc gcagcatcgc cgactgtccg cgcaacggcc gccagcccaa
     2581 tccggcaccg ttgtgcacgg cggtggtgct gccggcgttc gtcgaagggc tgcgcagcat
     2641 cgagggattc tcgcacctga tcatggtcta ctggatggat caggtgaaaa cgccggagct
     2701 cagcttcacg ccgccattcg atacggaagt gcgcgggatt tttgccacgc gttcgccacg
     2761 ccgcccgaat ccgatcgggc tgtcggtggt ggcgttcgag gggctggagg gtggcgatac
     2821 gttgaaggtg cgttatctcg attgtatcga cggcacgccg ctgctcgaca tcaagccgta
     2881 tctgaagagc acggattgcg agcctgattc gagcatgggc tggctggaac agcacgcgac
     2941 ccggcggcga taggcgatca gatggcgccc atcttgcgca ggtgtgaaat tttcccggcg
     3001 tcatagccga tttcctgcaa caaggtctcg gtgtcggccc ccaattccgg cgcgcgttcc
     3061 cgtggcattg cctcaccagg caaccggaac gctggcgcga ccatgcggaa gccgtcgtag
     3121 gaccacacgc gttcctcgtg cgtcacgaac tccgatccca acgcctgtgc cacatcgtag
     3181 acgggggccg cgggtactgc gcccgcgaag tgtgccagcc acgcctcggt tccgcgcatc
     3241 gacaacgccg catccagcaa ccgggtcagc tccgcgcggt tcttcagccg cgttttgaat
     3301 gtcgcgaaac gcggatcgtc tgaccactcg gcatggtcga tcttctggca cagcaccggc
     3361 cagaatttct gcttgttgca catgatgaag atccagccgt cagcggtgcg gtagagctgg
     3421 cttggcgtca gcgaaggatg tgcgccgcgc ggctcgcggc cctgcacgtg gccgccattc
     3481 aatgcccatg ctgcgaggta attcacgttc gccagcgccg tatcgaacag gctgacgtca
     3541 acatcgcgcc caacaccggt cgcgcgcgcc gatacgatgc cgccgagcag cgcggttgcc
     3601 gcatagacac cggccatcaa gtcgacaatc gacagcccga accgtgccgg cggctggtcc
     3661 ggttcgccgg tcaacgacag gtatccggcc tcggcctgca tcagatagtc gtagccaggc
     3721 caggctgcgc ggctgccctc gcgtccatac gcggagagat gcgcgcagac gatgcgcggg
     3781 tttgcctgct tcagctgatc gtaggtcaca ccgaggcgag gtggctggtc gccgcgcagg
     3841 ttgtccagca cgccgtccgc cgtcgccacc agatcacgga agacctgctg cccctcgggc
     3901 gtgcgcaggt tcaacgtgat ggagcgcttg ttgcggttga acgcctgaaa gaagtggctg
     3961 tcgtgctcgc caaggaaata ggggcctacc gagcggctta cgtcgccacc ttcggcgggg
     4021 ttctcgatct tgatcacctc ggcgcccatg tcggccagct gcatggaacc gaacgggcct
     4081 gcgccgtatt gctcgaccgc caggatgcga acgcctgcga gtggcagcat cagaccgggt
     4141 tccgcgccac gagctgccgc gcgatgatga tacgctgcag ttcgttggtg ccttcgccga
     4201 tcaccagcag gggcgcatcg cgatacaggc gttcgaccgg gaattccgtc gaatacccat
     4261 agccgccgtg gatacgcatc gcttcggccg cattctccat cgccgtctcc gtggcgaaca
     4321 gcttcgccat gcctgcttcc agatcgcagc gatcgccgcg gtcgtacatt tcggcggcgc
     4381 ggcgcacgag caggcgtgat gcctcggtgc gcgtcgccat atccgcgagc ttcaattgga
     4441 tcgcctgatg ctgcgcgatc ggctgtccga aggtcttgcg cagctgggcg tagcggagcg
     4501 actcgtccag cgccgcctgc gcgatcccca cgccgcgggc cgcgacgttg atgcgcccca
     4561 gttccagccc tgacagggcg tgctgcaagc ctttgccttc cacgccaccg atcagccgat
     4621 cggcgggaac gcggcaatcc tggaacacca gctccgcgct atcgattccc ttgtagccga
     4681 gcttctccag cttgcggctg acggtgaagc cggggccttt ttccgccagc agcatactca
//
LOCUS       DATGEM010000085         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_546074, whole genome shotgun sequence.
ACCESSION   DATGEM010000085 DATGEM010000000
VERSION     DATGEM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546074"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(715..1155)
                     /locus_tag="VKQ27_02855"
     CDS             complement(715..1155)
                     /locus_tag="VKQ27_02855"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HLY87903.1"
                     /translation="MDGILASPDAVQLAQVISQATAPAFMLGAGAGFVSILLGRMSAI
                     VDRIRHLNEIDEADRSRSHLKADLPRLRRRLRLLSDATRLILASGTCTALLLCVGFGS
                     AFLRLQHVYGAGALFFVAVVLMGIALVKFGQEVTIGISEADHYR"
     gene            1386..>2445
                     /locus_tag="VKQ27_02860"
     CDS             1386..>2445
                     /locus_tag="VKQ27_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87904.1"
                     /translation="MKRRAFLAAAAAGAALPLARPAIGGTAKTLVFVPQANLTSLDPV
                     WTTATVTRNFSLMVYETLYGRDQAFNPKPLMVQGHTIDDDGKRWTMKLREGLVFHDGT
                     PVLARDCVASLQRWLKRDAVSVTINARVDAIETPDDKTLVWRLKKPFPLLAHFLSKVQ
                     PQPVIVPERLAATDPFKQLTEIVGCGPFRFLPDELVSGHHAAFAKFDKYVSRQEPASY
                     MSGGHKVLLDRVEWRIIPDGATATNALVSGEVDWLELPSPDVIPVLKKASGVNTGLLD
                     IYGTVAILRPNSSIAPTNNVMLRKAMMAAVDPREAMIAAMGEDESGWHAPMGYFLPGV
                     AAANDAGMAFRTKPWSVDE"
BASE COUNT          466 a          697 c          844 g          438 t
ORIGIN      
        1 agcagcgatc ggtcaacgta gatcgcaaca agcgcgcctt catgccgcgt tctggacagg
       61 cgccagcaga tctgggcaac agggaatccg agccggtaga caacctggca cgcaatgtcg
      121 agcgatgtgg gggcgcgtgg ctgcgggaag ctatgcatcg aggaccgtgt aataaccagt
      181 cctcaatgaa tggataagtt ctttcatcga tgttgctggt gcgcacgggc cggcaccgca
      241 gaccactcat ccagcgagca ggtcttcaga ccgtctcggg cgcgccagac gcctcggcgg
      301 cgcggctcgt cacaatggcc agcaacgcgc cgcgtggtca gcctttcagt tccacggctt
      361 gggctggcgg cttatcggct ggatggcgcc tgcaggtaga ggcggaaggc tcggtggcct
      421 cgccggcggt ggctcgccgc gtgccagaaa gctgcgaact gtcgacctac tcattagcct
      481 gcccatctcg gcgtactccc ctgttcattc aataatcccc ggccgccggg gtaggtccga
      541 gagggtttcg acggggttgc agcctgccat cacgtcaccg tgatcgccgc ttgggtctcg
      601 gcgatttcgc gggtttcgag cgaacgcgtc ggaccggccg acatgccaac aacaggcgcg
      661 ttgttcggcg atgtatccgg aaaagcggaa cttcggggac agtcagtgta tcggctaccg
      721 ataatgatcg gcttcgctga taccgattgt cacttcctgg ccgaacttga cgagtgcgat
      781 acccatcagg acaaccgcca cgaagaataa cgcgccggcg ccgtatacat gctgcaggcg
      841 caggaaagcg gagccaaagc ccacgcaaag gagaagggcg gtgcacgtac cgctcgcgag
      901 gatgaggcgc gttgcgtcac tcaacaaacg gaggcgcctg cgcaagcgcg gaaggtctgc
      961 tttgaggtga gagcgcgatc tgtcggcctc gtcgatctcg ttgagatgcc gaatgcggtc
     1021 cacgatggcg ctcatacgcc ccagtaagat cgacacgaag ccggcaccgg cgccaagcat
     1081 gaatgccggt gccgttgcct gcgatatgac ctgtgcaagt tgaacggcat cgggcgatgc
     1141 gagaataccg tccatctgaa gactccccgc cggttcgcga atgaaaagac tgccgcagcg
     1201 agtcgggacg attcctccag atcgccgcgg tgcatagtgt gccgctggta ccctgcaagg
     1261 caagttcgtt gcgctccgag tcgaatcggg agcggcaaag gagagtactg cgtttccgtt
     1321 gcggtggggt gtggcgcggg cggccggata tgaatacgat cacgccaaac attaggaaac
     1381 gaccgatgaa acgacgcgcc ttcctggcgg ccgcggcggc gggcgccgcg ctgcctcttg
     1441 cccggccagc tatcggtggc accgccaaga ctctggtgtt cgtgccgcag gccaacctga
     1501 ccagcctgga tccggtgtgg accaccgcga ccgtgacacg caatttttcg ctgatggtgt
     1561 atgagacttt gtatggacgc gatcaggcgt tcaatccgaa gccgctgatg gtgcaaggcc
     1621 ataccatcga tgatgacggc aagcgctgga caatgaagct gcgtgagggg ttggtgttcc
     1681 acgacggcac gcccgtgctg gcgcgggact gcgtcgcttc attgcagcgg tggttgaagc
     1741 gcgacgcggt gagcgtgacc atcaacgcca gggtggacgc gatcgagacg ccggacgaca
     1801 agacgttggt gtggcggttg aagaagccgt tcccgctgct ggcgcatttc ctgtccaagg
     1861 tgcagccgca gccggtgatc gtgccggagc gcctcgcggc gaccgatccg ttcaagcaat
     1921 tgaccgagat cgtgggatgc ggaccgttcc ggttcctgcc ggatgagctg gtgtccgggc
     1981 accacgcggc gttcgcgaag ttcgacaagt atgtgtcccg gcaggagccg gcttcgtaca
     2041 tgtcgggtgg gcataaggtg ctgctcgatc gcgtggagtg gcggatcatt ccggatggcg
     2101 cgacggcgac gaacgcgctg gtctcgggcg aagtggactg gctggagctg ccttcgccgg
     2161 acgtgattcc ggtgctgaag aaggcgtcgg gcgtgaacac ggggctgctg gatatctacg
     2221 gcacagtggc gatcctgcgg ccgaacagtt cgatcgcacc gacgaacaac gtgatgctgc
     2281 gcaaggcgat gatggcggcg gtggatccac gcgaggcgat gatcgcggcg atgggcgagg
     2341 acgagtcggg ttggcatgcg ccgatgggat acttcctgcc tggtgtggcg gcggcgaacg
     2401 acgcagggat ggcgttccgg acgaagccgt ggagcgtcga tgagg
//
LOCUS       DATGEM010000086         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1000673, whole genome shotgun sequence.
ACCESSION   DATGEM010000086 DATGEM010000000
VERSION     DATGEM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000673"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2497)
                     /locus_tag="VKQ27_02865"
     CDS             complement(95..2497)
                     /locus_tag="VKQ27_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY87905.1"
                     /translation="MDKLAIRRASSVVLATSVSLLGLMAAPAHSQTSPDTGTLASGEI
                     VVTATKRATLIQNVPFSINAQTSQDIARAHAQTLEDLSRNVAGLSIQNLGPGQSQVAI
                     RGISSGQIVRDQPGVKEQVGVYLDESVISLSLFTPDLDLFDLNRVETLRGPQGTLFGA
                     GSEGGTIRYITNQPKFDRVEGSFEAGFNHIDGGNSGGDVKGAINLPLGETAAVRAVGY
                     YTRYAGFIDALHEDGSIKKNVNDGYRAGGRVAVTWKPIDALTITPRVIYQEIKTNGFN
                     REEKFNLFANPFTTTRPPVTLQDRQQFLLLPEEFRDKFLLVDNTATLDVGPASLTSVS
                     SYTHRNILVSRDASALTGSVSVDLGFPTAAVLLPSNLRDRTKLNQFTQELRVASNGEG
                     PLQWLVGGFYSHIRRNYSQRLPTPGYDAFTDAIFGAGTSAAVANGFGPDSPYNADLPY
                     KLRQLAAFAELTYNLTHTLHLTAGGRYYDYKETRRFHSGGLFSNDDDRRDSTTSNGAT
                     PRFLVSFDATPNMTFNAQVSKGFRLGGVNDPLNLPLCGPADAATFGGHPTFKDETLWN
                     YEGGLKAHFGPLRFNVAGFYNEIHNLQVTADAGSCSSRVIFNVPKAHSGGGEFEVAAT
                     PIKGLDLSLSGSLIDSKFDSTVTSSTGVELAGIRKGNRLPTVPKFQLSANGTYTVPVD
                     VFGSQEAFFTASVQHVGSRYTQPADQEGNPQSFVSHLPFGGATGNEATVLDLKLPAYD
                     LVNLSAGLRFPGQLEISVYVNNLTDKNALLSFDRERGGRARLGFEVNQPRTFGATLRK
                     SF"
     gene            complement(2763..>3016)
                     /locus_tag="VKQ27_02870"
     CDS             complement(2763..>3016)
                     /locus_tag="VKQ27_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY87906.1"
                     /translation="GLINRVVPDEQVDEEGLKIATELARGPRSLGLIRASAWAALETP
                     LEEQLARERNFQRDAGRTEDFVEGVTSFREKRPAAFKGC"
BASE COUNT          522 a          951 c          986 g          557 t
ORIGIN      
        1 ccgtgccggg ctgaacgggc aactccgccc gcaccgcctt catcccctca gccatccccc
       61 gccggccgcc tggaccgacg ggggattcgc ccgctcagaa gctcttgcgg agcgtcgcac
      121 cgaaggtgcg cggctggttc acctcgaagc caagtcgagc ccgcccgcca cgctcgcggt
      181 cgaacgagag cagtgcgttc ttgtcggtga ggttgttgac gtagaccgag atctccagct
      241 ggcccgggaa acgcagaccc gcgctcagat tgacgaggtc gtaggctggg agcttgaggt
      301 ccagcacagt cgcctcattg ccggttgcgc cgccgaacgg caggtgggaa acgaacgatt
      361 ggggattccc ctcctgatca gccggctgcg tatagcggct gccgacatgc tgcaccgagg
      421 cggtgaagaa ggcctcctgg ctgccgaaca cgtccaccgg cacggtgtag gtgccattcg
      481 ccgaaagctg gaacttgggc acggtcggca ggcggttgcc cttgcggatg cccgccagct
      541 cgacgcccgt gctgctggtc accgtggagt cgaacttcga gtcgatcagg ctgccggaca
      601 gcgacaggtc gagccccttg atcggtgtcg ccgccacttc gaactcgccg ccgccggaat
      661 gggccttcgg cacgttgaaa atcacgcgcg acgagcagga gcccgcgtcc gccgtgacct
      721 ggaggttgtg gatttcgttg tagaacccgg ccacgttgaa gcgcagcgga ccaaagtgcg
      781 ccttcaggcc gccttcatag ttccacagcg tttcgtcctt gaaggtcgga tggccgccga
      841 aggtcgccgc atccgccggc ccgcacagtg gcaggttgag cggatcgttc acgccgccca
      901 gacggaagcc cttcgacacc tgcgcgttga aggtcatgtt gggcgtggcg tcgaagctta
      961 ccaggaagcg cggggtggcg ccgttcgacg tggtggaatc gcgcctgtcg tcgtcgttcg
     1021 agaagaggcc gcccgagtgg aagcggcgcg tttccttata gtcgtaatag cggccgcctg
     1081 ccgtcaggtg cagcgtgtgc gtgaggttgt aggtcagttc ggcgaaggcc gcgagctgac
     1141 gcagcttgta gggaagatcc gcattgtacg gcgaatccgg cccgaagcca ttggcgaccg
     1201 ccgccgaggt gccggcgccg aaaatggcgt cggtgaacgc atcatagccg ggcgtaggca
     1261 ggcgctgcga atagttgcgc ctgatgtgcg agtagaagcc gccgaccagc cactgcaacg
     1321 gcccctcgcc gttcgacgca acgcgaagct cctgcgtgaa ctggttgagc ttcgtgcggt
     1381 cgcgcaggtt ggacggcagc aataccgccg ccgtcgggaa gccgagatcg accgacacgc
     1441 tgcccgtcag cgcgctcgca tcgcggctga cgaggatgtt gcggtgcgta tagctgctca
     1501 ccgaggtcag gctcgctgga ccgacatcga gcgtcgcggt gttgtcgacc agcaggaact
     1561 tgtcgcggaa ctcctcaggc agcagcagaa attgttggcg atcctgcagc gtcaccggcg
     1621 gccgcgtggt ggtgaacgga ttggcgaaga ggttgaactt ctcctcgcgg ttgaagccgt
     1681 tggtcttgat ctcctgatag atcacgcgcg gcgtgatggt cagcgcgtcg atcggcttcc
     1741 aggtgacggc gacgcggccg ccggcacgat agccgtcatt gacgttcttc ttgatgctgc
     1801 cgtcctcgtg cagcgcatcg atgaagccgg cgtagcgggt gtaatagccc accgcgcgca
     1861 ccgccgcggt ttcgcccagc ggcagattga tcgcgccctt gacgtcgccg ccgctgttgc
     1921 cgccgtcgat atgattgaag cccgcctcga acgatccttc gacgcgatcg aatttcggct
     1981 ggttggtgat gtagcggatc gtgccgcctt ccgatcccgc tccgaacagc gtgccctgcg
     2041 gcccgcgcag cgtttcaacg cggttcaggt cgaacaggtc gagatcgggc gtgaacagcg
     2101 acagcgagat caccgattca tcgagataga cgccgacctg ctccttcacg cccggctgat
     2161 cgcgcacgat ctggcccgag gaaatgccgc ggatcgccac ctggctctgc cccggcccga
     2221 gattctggat gctgaggcca gcgacgttgc gcgacagatc ttccagcgtc tgcgcgtggg
     2281 cgcgggcgat atcctgcgaa gtctgcgcgt tgatcgagaa gggaacgttc tggatcagcg
     2341 tcgcacgctt ggttgcggtc acgacgattt cgccgcttgc caacgtgccc gtatccggac
     2401 tggtctggga atgcgcaggt gctgccatga gaccaagcag ggaaacgctg gtcgcaagca
     2461 ccacggacga tgcgcgacgg atagccaact tgtccatata ccccccttga tagatgtcgg
     2521 tctttgaccg atcgtgggga catccttccc tgttggcatt tgtccgtcaa gacagccgca
     2581 tccatacctc caatgacttc gctgtctttt tgctattttt gtaaatatct gtgcggataa
     2641 gccacagatt gacggttcat ggcaattgtt acgcaacgat attcccgggc gcaccgatcg
     2701 gaccaccggg cgacgcgaat gcgggaacga agtgtcgcgc cggtaccgtg cagaaggtcc
     2761 gttcaacacc ccttgaaggc ggcgggacgc ttctcgcgga acgacgtcac gccttcgaca
     2821 aagtcttcgg tccggccggc atcacgctgg aagttgcgct cgcgcgccag ctgctcctcc
     2881 agcggggttt cgagcgccgc ccaggcggac gcccggatca agcccagcga acgcggcccg
     2941 cgcgccagct cggtggcgat cttgagcccc tcctcgtcga cctgctcgtc ggggacgacg
     3001 cggttgatca gccccc
//
LOCUS       DATGEM010000087         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_317320, whole genome shotgun sequence.
ACCESSION   DATGEM010000087 DATGEM010000000
VERSION     DATGEM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317320"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VKQ27_02875"
     CDS             <1..1139
                     /locus_tag="VKQ27_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87907.1"
                     /translation="MSDSADFIQRARDLAPILREAGDEIETRRELPHHVVTALIAGGF
                     FRMLQPRFLGGAELRPILFSQVTEALAQTNASVAWCVGQNNGCSMSAAYLDRGVATEI
                     FGPTTGILAWGPPGAPFEAQPVEGGYRISGRWRFASGCHHASWLGAHMPIAGTGGKVR
                     TMLFPKSSVQMNDIWHTIGLRGTASNEYVVKDLFVPQRFTMARDTAAERREDGLLYRF
                     TSNQLYSCGFAGVGLGIARGTIDDFLELPANKVSRGASRPMRENNVVQSQLAQCEARW
                     RSARAFLHTTWETAWQHVEATGQQTGEDQAMIRLASTWAIQQSREIVNTLYHATGSMA
                     VFEEQPFEQRLRDIHTVAQQSQGRQLHYESVGQILFGMEPENQF"
     gene            1153..1563
                     /locus_tag="VKQ27_02880"
     CDS             1153..1563
                     /locus_tag="VKQ27_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87908.1"
                     /translation="MPAMSLNHYTILARDLEATKNFYTDIVGLTVGDRPPLGFPGYWL
                     YCGGVPTVHLIGHRPENEPIPDAASDPAKTGRLDHIAFSCEGLKDMKTNLEGRKIKYD
                     ERVLPRLNMTQLFYYDPDGIAVECNFPAAETPSR"
     gene            1614..2675
                     /locus_tag="VKQ27_02885"
     CDS             1614..2675
                     /locus_tag="VKQ27_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87909.1"
                     /translation="MAPLRTAVLMKTDIANSTPKFRALLAEDHQELLRRHRAFIAAHA
                     AGEGGQIIRPTGDGFWLEFPSVTAAARAAMAMQTALRLERPTSGGERLAMRVVIGVGD
                     VSELEGEIIGELLALIVRIETVTPADEIYLTPTARLALVSAEVQTSHVDSFALKGFSE
                     PVEVYRVEQRHRSCVLPDACILLTDLRGFTRLTETAPIAMIEQALAALDELVTATARQ
                     FGGVIRYSVGDSFCVTFDTAIQGMRATERLSAEWEAANRRERIGCAINLVLHRGYLCG
                     FRSFLYGKGIGEASRVQQASVELLANDEGGVFLTEAVRRDLADSHWHDHIVPVTLSLP
                     DVVAYRLVVTEQAIQPPAG"
BASE COUNT          504 a          815 c          913 g          483 t
ORIGIN      
        1 gtatgagcga tagcgccgac ttcatccaac gcgcccgtga tctcgcgccg atactgcgcg
       61 aggcagggga tgaaatcgaa acacgacgag aactgccgca tcatgtcgtg acggcgctga
      121 tcgccggtgg ctttttccgg atgttgcaac ctcggttcct gggaggcgcg gagctgcggc
      181 cgatattgtt ctcccaggtc acggaggcac ttgcccagac aaatgcttcg gttgcctggt
      241 gcgtgggcca gaataacgga tgctcgatgt cggcggcgta tctggatcga ggggtcgcga
      301 cagagatatt cggaccaacc accgggatcc ttgcgtgggg gccaccgggc gcccccttcg
      361 aggcgcaacc ggtcgagggt ggctaccgga tcagcgggcg ctggcggttc gcgagcggct
      421 gccatcacgc aagctggctc ggcgcgcaca tgccaattgc ggggaccggc ggcaaggtgc
      481 ggacaatgct gttcccgaag tcgagcgtgc agatgaacga catctggcac acgatcgggc
      541 tgcgtggcac ggccagcaat gaatatgtgg tgaaggacct gttcgtgccg cagcgcttca
      601 cgatggcgcg cgacacggcg gcagagcggc gcgaggacgg gctgttgtac cgtttcacca
      661 gcaaccagtt gtattcgtgt ggatttgccg gcgtcggact ggggatcgcg cgcggcacca
      721 tcgacgattt cctcgagttg ccggcgaaca aggtctcacg cggggcgtcg cgaccgatgc
      781 gcgagaataa cgtggtgcag tcgcagttgg cgcagtgcga ggcgcgctgg cgttcggcgc
      841 gcgccttcct gcacacgacg tgggaaaccg cctggcagca tgtcgaggcg acgggacagc
      901 agaccggcga ggatcaggcc atgatccggc tggcctccac ctgggcaatc cagcaatcgc
      961 gagaaatcgt caatacgctg tatcatgcga ccggatcgat ggcggtgttc gaggagcagc
     1021 cgttcgagca gcgcctgcgc gacatccaca cggtggcgca gcagtcgcag ggacggcagc
     1081 ttcactacga atcggtcgga cagatcctgt tcggcatgga gccggaaaac cagttctgag
     1141 gaggaaacac agatgcccgc gatgtcactc aatcactaca cgatactggc ccgtgacctg
     1201 gaggcaacga agaatttcta cacggatatt gtcgggctga ccgtgggcga tcggccgccg
     1261 ctgggcttcc ctggatactg gctctattgc ggcggcgtgc cgacggtcca cctgatcggc
     1321 catcggccgg agaatgagcc gatccctgac gcggcgagcg acccggcgaa gaccgggcgg
     1381 ctggatcaca tcgccttctc ctgtgagggg ctgaaggaca tgaagacgaa tctcgaaggg
     1441 cgcaagatca agtacgatga gcgcgtgctg ccgcggctga acatgacgca gttgttctac
     1501 tacgacccgg atggcatcgc ggtggagtgc aacttcccgg ccgcggagac gccgtcccgc
     1561 tgaccctgcg actcgcgatg gagccgggtg gctgctgacg agagggggag ccgatggccc
     1621 cgctgcgtac cgctgtcctg atgaagaccg acatcgccaa ctccaccccc aagttccggg
     1681 ccctgctggc ggaggatcac caagaactgc tgcgccgtca ccgtgcgttc atagcggcgc
     1741 acgcggccgg cgaaggtggc cagatcatcc gcccgaccgg cgacggtttc tggctggagt
     1801 ttccgagtgt gaccgccgcg gccagggcgg cgatggcgat gcagacggca ttacggttgg
     1861 aacgaccgac atccggaggg gaacgcctgg cgatgcgagt cgtcatcggc gtcggcgacg
     1921 tcagcgagct cgaaggtgag atcatcggcg aattgctggc actcattgtc cgcatcgaga
     1981 ccgttacgcc ggctgacgag atctacctca cgcccactgc ccggctggcc ctcgtgtcgg
     2041 ccgaagtgca gacgtcgcat gtcgacagct tcgcgctgaa gggcttttcc gaacctgtcg
     2101 aggtatatcg cgtcgagcag cgacatcggt cgtgcgtctt gccggacgcc tgcattctgc
     2161 tgactgatct tcggggattc acccggctga ctgaaacggc gccgatcgcc atgatcgagc
     2221 aggcacttgc cgcactcgac gagctggtca ccgcgacagc acggcagttc ggcggcgtca
     2281 tacgctacag cgtcggtgac agcttctgcg tcaccttcga cacagcgatc cagggcatga
     2341 gggcaacgga acgactgagc gcggagtggg aagcggcaaa ccggcgtgag cgcatcggct
     2401 gtgctatcaa tctcgtttta catcggggtt atctctgcgg ctttcgttct tttctttacg
     2461 gcaagggcat cggtgaggcc tcgcgcgtcc agcaggcgtc cgtcgagtta ttggcgaatg
     2521 atgaaggcgg cgtcttcctt accgaggcgg ttcgcagaga cctggctgac agccattggc
     2581 acgatcacat cgtcccggtc acgctgagcc tgcccgacgt ggtcgcctac cgcctggtgg
     2641 tcaccgagca ggctattcag ccgccagccg gctgactgcg gcatccttga tcgagacgcg
     2701 ccaatggatg cggtc
//
LOCUS       DATGEM010000088         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1455415, whole genome shotgun sequence.
ACCESSION   DATGEM010000088 DATGEM010000000
VERSION     DATGEM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455415"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VKQ27_02890"
     CDS             <1..419
                     /locus_tag="VKQ27_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY87910.1"
                     /translation="MIKQKRGAVVNMASVAGLVASPGMPAYVASKHAVIGLTKTAAGE
                     VARAGVRINAVCPGPIDTRMIHSVEAMLDPKDPGSVSARYQSNIPLGRYGSAEEIANL
                     VLFLCSDLASNMTGGHYVSDGGRTATGGAVSNTLIK"
     gene            436..753
                     /locus_tag="VKQ27_02895"
     CDS             436..753
                     /locus_tag="VKQ27_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87911.1"
                     /translation="MITAIVLYDLPEEIDRDACRAHFLKIAPGFLGVPGFIRKQFIHD
                     VNGRVAGGSYLWQDLESAKAFYSGAWLEGIRQRYRCEPRISYYETFAIADRATGLAGA
                     PLD"
     gene            759..1652
                     /locus_tag="VKQ27_02900"
     CDS             759..1652
                     /locus_tag="VKQ27_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY87912.1"
                     /translation="MKTIRTPVLEIAFHEYGSADGVPVILLHGWPSDPHDWDDVAPAL
                     AGDGCRVLVPWLRGYGPTRFLDPATPRSGQQAALGSDVRDFMDALGMQRAVLGGYDWG
                     GRGACVASALWPERVRALVSITGYNIQNIAASGRPVSAAQEHRYWYQWYFQTERGRAG
                     LQQNRRDVCRLLWQLWSPNWRFDDATFDATAASFDNPDFVDVTIQSYRHRYGNAPGDP
                     AYDALEQLLAGQPPITVPTIVLHGACDGVGPAEQSERHARHFTAGYERRVVPVAGHFL
                     SRETPGAVVQAVRDVLAGGTV"
     gene            1649..3283
                     /locus_tag="VKQ27_02905"
     CDS             1649..3283
                     /locus_tag="VKQ27_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HLY87913.1"
                     /translation="MNSAYLTGLDRNQANYTPLTPLTFIERAAYVFPDRPSVVHGAQR
                     FTWRETYGRCRRLASALARRGIGKNDTVAVMAPNIPATYEAHFGVPMCGAVLNTLNTR
                     LDAETIAFMLRHGGARVLITDREFSATIEQALALLEEPPLVIDIDDPSYQGGKTLGAI
                     GYEAFLAEGEPDYAWQAPADEWDAISLNYTSGTTGDPKGVVYHHRGAYLNAISNVLDW
                     DMPTHAVYLWTLPMFHCNGWCFPWTVAERAGTSVCLRRVEAKAALDAIREHRVTHMCG
                     APIVYSMLINAPEELRREIDHQIAGQIAGAAPPAAIIEGSEKIGIELTHVYGLTETYG
                     PASVCAKHENWSMMPIDKRAERNGRQGVRVPMQEALTVLDPETMRPVPWDGETMGEIM
                     FRGNITMKGYLKNPKATTAAFGGGWFHTGDLAVMQPDGYVKIRDRSKDIIISGGENIS
                     SIEVEETLYRHPAVLSAAVVAQPDEKWGETPCAFIELKPGAGVTADELRAFCREHMAR
                     FKVPKSFVFRELPKTSTGKIQKFVLRELAKSASAIE"
BASE COUNT          574 a          928 c         1247 g          619 t
ORIGIN      
        1 tgatgatcaa gcagaagcgc ggcgcggtgg tgaacatggc gtcggtggct gggctggtgg
       61 cctcgcccgg gatgccggcc tacgttgctt cgaagcacgc ggtgatcggc ttgaccaaga
      121 cggccgccgg agaagtggcg cgagccggcg tgcgcatcaa tgcggtgtgt cccgggccga
      181 tcgatacgcg catgattcat tcggtggagg cgatgctgga tccaaaggat ccgggcagcg
      241 tgagtgcgcg ctatcaatcc aatattccgc tcggccgtta tggttcggcg gaggagatcg
      301 ccaacctggt gctgttcctg tgttccgacc tggcgagcaa catgaccggc gggcactatg
      361 tgtctgacgg cggacgcacc gcgacaggtg gtgcggtgag caacacgctg atcaagtgag
      421 gggaggggcg gcaggatgat cacggccatc gtgctctatg acttgccgga agaaatcgac
      481 agggatgcct gccgcgcgca tttcctgaag atcgcccccg ggttcctggg ggtgcctggt
      541 ttcatccgca agcagttcat ccatgacgtg aacgggcgcg tggcgggtgg ttcgtatttg
      601 tggcaggacc tggaatcggc gaaagcgttc tatagcgggg cgtggctgga gggcatcagg
      661 cagcggtacc gctgcgagcc gcgcatcagc tactacgaga cgttcgcgat tgccgatcgg
      721 gcgacgggcc tggccggcgc gccgctggac tgatgcgcat gaagacgatc cggacgccgg
      781 tgctggagat tgcctttcac gagtacggtt ccgctgacgg cgtgccggtg atcctgctgc
      841 acggatggcc atccgatccg catgactggg atgacgttgc gccggcgctg gcgggtgatg
      901 ggtgccgcgt gctggtgcca tggctgcgtg gctacgggcc gacgcggttc ctggaccctg
      961 cgacgccgcg gtccggacag caggcggcgc ttgggtcgga tgtgcgtgac ttcatggatg
     1021 cgcttggcat gcaacgggcg gtgctgggcg gctacgattg gggtgggcga ggcgcgtgcg
     1081 ttgcgtccgc tttgtggccg gagcgggtgc gcgcgctggt ctccatcacc ggttacaaca
     1141 tccagaacat tgcggcgtcc gggcgcccgg tgtcggcggc gcaggaacat cgctactggt
     1201 accagtggta tttccagacc gagcgcggcc gtgccgggtt gcagcagaac cgccgcgatg
     1261 tttgccggct gctgtggcag ctctggtcgc cgaactggcg gttcgacgat gcgacgttcg
     1321 acgcgacggc cgccagtttc gataatccgg atttcgtcga tgtgacgata cagtcgtatc
     1381 ggcatcgcta tggcaatgcg ccaggtgatc cagcctatga tgccttggag cagctgctgg
     1441 ccgggcagcc gccgatcact gtgccgacga tcgtgctgca tggcgcgtgc gacggtgtcg
     1501 gaccggcgga acaatcggaa cggcacgcgc ggcatttcac cgcgggttat gagcggcggg
     1561 tggttcccgt ggccgggcat ttcctgtcgc gcgagacgcc aggggccgtg gtgcaagcgg
     1621 tgcgcgacgt gcttgcggga ggaacggtat gaacagcgct tacctgaccg gcctcgaccg
     1681 gaaccaggcc aattatacac cgctgacgcc gctgacgttc atcgagcgcg cagcctatgt
     1741 ttttcccgac cggccgtccg tggtgcatgg tgcgcagcgg ttcacatggc gcgagacgta
     1801 tgggcggtgc cggcgacttg cctcggcgct ggcacggcgg gggattggca agaacgatac
     1861 cgtcgcggtg atggcgccca atatcccagc gacgtacgag gcgcatttcg gcgtgccgat
     1921 gtgcggtgcg gtgttgaaca cgctgaatac gcggctcgat gccgagacga tcgccttcat
     1981 gctgcggcac ggcggcgcgc gggtgctgat caccgaccgc gagttctccg ccacgattga
     2041 acaggcgctg gcgttgctgg aggaaccgcc gctggtgatc gatatcgatg atcccagcta
     2101 tcagggcggc aagacgctcg gcgcgatcgg gtatgaggcg tttctggcgg agggggagcc
     2161 agactatgcg tggcaggcgc cggcggacga gtgggatgcc atttcgctga attatacatc
     2221 cggcaccacg ggtgatccga agggcgtggt gtatcaccat cgtggcgcgt atctgaatgc
     2281 gatcagcaat gtgctggatt gggatatgcc gacgcacgcg gtgtaccttt ggacgctgcc
     2341 gatgttccac tgcaacggct ggtgctttcc gtggactgtc gcggaacgcg ccggtaccag
     2401 cgtttgcctg cggcgtgttg aggcgaaggc ggcgctggat gcgatccgcg agcatcgcgt
     2461 gacgcatatg tgtggcgcgc cgatcgtgta ttcgatgctg atcaatgcgc cggaggaact
     2521 gcggcgcgag atcgaccacc agattgccgg gcagatcgcg ggggcggcgc cgcctgctgc
     2581 gatcatcgaa gggtcggaga agatcgggat cgagcttacg catgtgtatg ggctgacgga
     2641 gacttatggg ccggcgtcgg tgtgcgccaa gcatgagaac tggagcatga tgccgatcga
     2701 taagcgcgcc gagcggaacg ggcggcaggg tgtgcgtgtg ccgatgcagg aagcgctgac
     2761 ggtgcttgat ccggagacga tgcggccggt gccgtgggac ggcgagacga tgggcgagat
     2821 catgttccgc ggcaacatca cgatgaaggg gtatctgaaa aatcccaagg caacgaccgc
     2881 ggcttttggc ggtgggtggt tccatacggg tgatctggcg gtgatgcagc cggacggcta
     2941 tgtgaagatc cgcgaccggt cgaaggacat catcatatcg ggcggcgaga acatctcctc
     3001 gatcgaggtg gaggagacgc tgtaccggca ccccgctgtg ctgagcgcgg cggtggtggc
     3061 gcagccggac gagaagtggg gcgagacgcc ttgcgccttc atcgagctga agccgggcgc
     3121 cggggtcacc gcggatgaac tgcgcgcgtt ctgccgcgag cacatggcgc ggttcaaggt
     3181 gccgaagagc ttcgtgtttc gggagttgcc gaagacctcg accgggaaga tccagaagtt
     3241 cgtgctgcgc gaactggcga agtcggcgag cgcgatcgag tagacagcag cctgtccacg
     3301 cgttcgcgca gagccagggc gtcgaacggg gcgcggactt tccggtcgtt gtcgaaatag
     3361 acgaacac
//
LOCUS       DATGEM010000089         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_654397, whole genome shotgun sequence.
ACCESSION   DATGEM010000089 DATGEM010000000
VERSION     DATGEM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654397"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VKQ27_02910"
     CDS             complement(<1..117)
                     /locus_tag="VKQ27_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLY87914.1"
                     /translation="MQIAMIGLGRMGANMAQRLLRGGHEVVGFDPAEAARKLI"
     gene            complement(250..1356)
                     /locus_tag="VKQ27_02915"
     CDS             complement(250..1356)
                     /locus_tag="VKQ27_02915"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HLY87915.1"
                     /translation="MTQSYAYVGSYTPNGGGIYLFRIDRATGALAQIQIVDDVRNPTW
                     LAMSPDQTRLYAISEIDNYEGTKNGAVVSYAVEADSLRLTRLGAVSSAGAAPVFVSVH
                     PSGKFVFVANFGGGTVAVFPVAAGGSLSGASDVRPSVGARHRARAVDDPPGQFAASDH
                     DGPHLHMVAPDPSGQFVIANDAGLDLTLVWRFDAQAGRLLPAEVPVIAAPSGSAPRHF
                     VFHPNGKLFYNLYEHDAKVAVYDYDGATGRMRHKQTAAATPQKFAGSILSSAIRMTAD
                     GRYLYVANRMHSSLCAFAVAADGQLRMIGDTWAHADSPRSIAIDPASGLLFSCNQKGD
                     SITSFRINASNGTLQFTGRYEPLGSPAFITLLQR"
     gene            complement(1467..2711)
                     /locus_tag="VKQ27_02920"
     CDS             complement(1467..2711)
                     /locus_tag="VKQ27_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY87916.1"
                     /translation="MTPVPTAAPGTAATILGPAAPTAAPAATTPAYTATPYVSPWMDD
                     DLVMFRDAAARFVEAEMVPNEARWREQQNVGREIWRKAGTTGLLCTDIAAEYGGVGGD
                     FRHEVVLYEELGRRGLSGFGQGVHSICAHYLANHGTDDQKKRFLPRMAGGELIGAIGM
                     TEPSAGSDLQGLKTRAVRDGGHYVVNGSKIFITNGYLAGLIALVVKTRPGDGAKGTSI
                     LMVETQDLKGYRVGRILDKMGMKSQDTAELFFDDVCVPADNLLGGSEGQGFYQLMADL
                     PYERVLIAVAAVAAMEGAVAATIAYAKERKAFGKSIAEFQNTKFKLAEISTKTRIARV
                     FVDDCISKVVAGTLDTVTASMAKYWVTDAQQEVLDECVQLHGGYGYMNEYMVCRMFAD
                     ARVQRIYGGTNEIMKEVISRAL"
     gene            complement(2711..2872)
                     /locus_tag="VKQ27_02925"
     CDS             complement(2711..2872)
                     /locus_tag="VKQ27_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87917.1"
                     /translation="MAKPNYRHAKKQKEAARKARQDAKQQRRAHTPDSQDASVPAAPA
                     EAAPVKSEV"
     gene            complement(2909..3805)
                     /locus_tag="VKQ27_02930"
     CDS             complement(2909..3805)
                     /locus_tag="VKQ27_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLY87918.1"
                     /translation="MSVSVRYRVRHETVYSYGGDVAHSHQLLHLIPRAGLRQQCHVCT
                     IAVTPEPSVRRDDLDAFGNHVTRLEYDLPHDRLEVLAEAGVEVQAAPTTESEASDEWE
                     AVGGSLAFSGRPMHEGLLDACKYRMESSYIRIKQTFSDYAADCFPPKRPVLAAADALM
                     HKIHREFQYAPGSTTIRTSALEAFEARRGVCQDFAHIMIGCLRSRGLAARYVSGYLRT
                     LPPAGADAAYVGADASHAWVSVFCPPFGWIDLDPTNDVRVSSDHIVIAWGRDFGDVSP
                     LRGVIVGGGRHRLSVRVSVQAE"
     gene            complement(3802..>4497)
                     /locus_tag="VKQ27_02935"
     CDS             complement(3802..>4497)
                     /locus_tag="VKQ27_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HLY87919.1"
                     /translation="QRLQWCAAQTRSRLSTENWRAISVLQRQFQEAAERRRDPRETLD
                     ALLLSLAALAGFALDDMTQDVGWRLLMLGRRLERMQFLAGLLARSLKSGATPTQGELE
                     WLLDIGDSTITYRTRYLASPLLGSTIDLLVFDKTNPRALAYQWNHMQYSLVRIAASLG
                     GTPDDSIDEAVAAVERLELAAVDGDSARAGRAREALAAQLDTLAGAAGRLSDRLSLKH
                     FSLIDIETRTVAA"
BASE COUNT          760 a         1487 c         1457 g          793 t
ORIGIN      
        1 gatcagcttg cgcgccgcct ccgcgggatc gaagcccacc acctcatgcc ccccgcgcag
       61 caggcgctgc gccatgttcg cgcccatgcg gcccaaacca atcatcgcga tttgcataat
      121 ttacaacctc gttgcgcacg aattgagagg acctgcgatg gagtatatcc gcgtcgggag
      181 ccgggctgaa tatttcctcg ggccggccgt ccgaccggcg cgcgagcgtg acgcgcggcg
      241 gcgctgcggc tatcgctgca gcagcgtgat gaaggcgggg ctgcccaagg gctcgtaacg
      301 ccccgtgaat tgcagcgtgc cgttcgacgc gttgatgcgg aacgaggtga tcgagtcgcc
      361 cttctgattg caggaaaaca acaggccgct cgccggatcg atggcgatgc tgcgcggcga
      421 atctgcatgc gcccaggtat cgccgatcat ccgcagctgc ccgtcggcgg ccacggcaaa
      481 tgcgcacagg ctgctgtgca tccggttggc gacgtagagg tatcgcccat ccgccgtcat
      541 gcggatcgcc gacgacagaa tgcttccggc aaatttctgc ggggttgcgg cggcggtctg
      601 cttgtgacgc atgcggcccg tcgcaccgtc gtagtcataa acggcgacct tggcatcgtg
      661 ttcgtacaag ttgtagaaca gcttgccgtt gggatggaac acgaagtgcc gcggggccga
      721 gcccgacgga gcggcgatca ccggcacctc ggccggcagc agacgtcccg cctgcgcgtc
      781 gaagcgccac accagcgtca gatcgaggcc cgcatcgttg gcgatcacga attggccgga
      841 aggatcggga gcgaccatgt gaagatgcgg cccgtcatga tcgctcgccg caaattgtcc
      901 gggaggatcg tcgacggcgc gcgcgcggtg gcgggcgccc acgctggggc gcacgtccga
      961 tgcgccgctc aagctgccgc cggctgccac cggaaaaacc gccacggtgc ctccgccgaa
     1021 attcgcgacg aagacgaact tcccggaggg atgcacgctg acgaagacgg gcgcggcgcc
     1081 ggccgaactc acggcaccca agcgggtcaa ccgcaggctg tccgcctcca ccgcgtatga
     1141 cacgaccgcg ccgttcttcg tgccttcgta gttgtcgatc tcgctgatgg cgtacagccg
     1201 cgtttggtcc gggctcatgg cgagccatgt gggattgcgg acgtcgtcga cgatctggat
     1261 ctgggccaag gcgccggtcg cgcgatcgat ccggaacagg tagatgccgc cgccgttcgg
     1321 ggtgtagctg ccgacgtacg cgtaggactg cgtcactcgc gcttccgccg gcgccttggc
     1381 cgcaggcacg atcagtagtg ccgtcgttgc acggacgaaa tcccggcgtg tgattcccga
     1441 tctcaccgct tcgcggcggt cccccgctac aaagcgcggg agatcacctc tttcatgatt
     1501 tcgttggtgc cgccgtagat gcgctgcacg cgcgcatccg cgaacatccg gcataccatg
     1561 tattcgttca tgtaaccgta gccgccgtgc agttgaacgc actcgtcgag cacttcctgc
     1621 tgcgcatcgg tgacccagta cttcgccatc gacgcggtca cggtatcgag cgtgccggcc
     1681 acgaccttgg agatgcagtc atcgacgaac acccgcgcga tgcgcgtttt cgtcgaaatt
     1741 tcagcaagct tgaacttcgt gttctgaaat tccgcgatcg atttgccgaa tgccttgcgc
     1801 tccttggcat aggcgatggt cgcggcgacg gcaccttcca tggcggccac cgccgcaacg
     1861 gcgatcaaga ctcgttcgta gggcaggtcc gccatcagct ggtagaaccc ctgaccctcg
     1921 ctaccgccaa gcagattgtc ggccggcacg cacacgtcgt cgaagaacaa ttcggccgtg
     1981 tcctgggatt tcatgcccat cttgtcgagg atgcggccga cgcggtagcc cttcagatcc
     2041 tgggtctcca ccatgaggat ggaggtgccc ttggcgccgt cgcccggacg ggtcttcacc
     2101 accagtgcga tcaatcccgc cagatagccg ttggtaatga agattttcga gccattgacg
     2161 acgtagtgac cgccgtcgcg cacggcacgg gtcttgagac cctgcagatc ggagccggcc
     2221 gatggttcgg tcatgccgat ggcgccgatg agttcgccgc cggccatgcg cggcagaaag
     2281 cgttttttct gatcgtcggt gccgtgattg gccaggtaat gcgcacagat gctgtgcacg
     2341 ccctgaccga agccgctcag gccgcgccgc cccagctcct cgtacagaac gacctcatgg
     2401 cggaagtcgc cgcccacccc gccatattcc gcggcgatgt cggtgcacag cagaccggtc
     2461 gttcccgcct tgcgccagat ttcccggccg acattctgct gttcccgcca gcgcgcctcg
     2521 ttcggtacca tttccgcctc gacgaatcga gctgctgcat cgcggaacat caccaggtcg
     2581 tcgtccatcc acggagagac gtagggggtg gccgtgtagg ctggcgtggt cgcggccgga
     2641 gctgcggtag gggccgcagg gcccaggatg gtcgccgccg tgcccggagc ggccgtcggg
     2701 accggggtca tcaaacctcc gacttgacgg gcgctgcctc cgcgggcgcc gcgggcaccg
     2761 acgcatcctg tgaatcgggc gtgtgcgccc ggcgctgctg cttcgcatcc tggcgcgcct
     2821 ttctcgcagc ttccttctgc ttcttcgcgt gacggtaatt cggcttggcc aacttctgtc
     2881 tccttggaac ctgctcgtgt gatcgatctt actctgcctg tacggaaacg cgtaccgata
     2941 agcgatgacg gccgccgccg acgatcacgc cgcgcagcgg cgatacgtcg ccgaagtccc
     3001 gcccccacgc gatgacgatg tggtcggagc tcacgcgcac atcgttggtc gggtccagat
     3061 cgatccagcc gaagggtgga caaaatacgg atacccaggc gtgcgaagcg tcggcgccga
     3121 cgtaggcggc gtccgccccg gccggaggca gtgtgcgcag atagccgctg acgtatcgcg
     3181 ccgccaggcc ccgcgaacgc aagcagccga tcatgatgtg ggcaaagtct tggcacacgc
     3241 cccggcgggc ctcgaacgct tcgagggcgg acgtgcggat cgtggtgctg cccggcgcgt
     3301 attggaattc gcggtggatt ttgtgcatca acgcgtccgc cgcggcgagg acggggcgct
     3361 tcggcgggaa gcaatccgcc gcatagtcgc tgaacgtctg cttgatccgg atgtagctcg
     3421 actccatgcg gtacttgcag gcgtccagca gtccttcgtg catcggcctg cccgagaagg
     3481 ccagcgagcc gcccaccgcc tcccactcat cgctcgcctc ggactccgtc gtaggcgccg
     3541 cctgaacctc gacgccggcc tccgccagca cctcgagcct gtcgtgcggc agatcgtatt
     3601 cgaggcgcgt cacgtgattg ccgaaggcgt cgaggtcatc gcggcgcacg ctgggctccg
     3661 gcgtcacggc gatggtgcag acatggcact gctgccgcag cccggcgcgc gggatcagat
     3721 gcagcagctg atgcgaatga gccacatcgc cgccgtagct gtacacggtt tcatggcgca
     3781 cccggtagcg aacggagacg ctcatgcggc cacggtgcgc gtttcgatgt cgatgagcga
     3841 aaaatgcttc agcgacagcc ggtcggaaag ccggccggcg gcgcccgcca acgtatcgag
     3901 ctgcgcagcg agcgcctcgc gcgccctgcc ggcgcgtgca ctgtcgccat cgaccgcggc
     3961 gagctccaaa cgttcgacgg cggccacggc ctcatcgatg ctgtcatccg gggtgccccc
     4021 gagggaggct gcaatccgca ccaacgaata ttgcatgtga ttccactggt acgcgagggc
     4081 gcgcggattg gtcttgtcga acaccagaag gtcgatggtg gaacccagaa gcggcgatgc
     4141 cagatagcgc gtgcgatagg tgatggtgct gtcgccgatg tcgagcagcc actccagttc
     4201 gccctgagtg ggcgtggcgc cggacttcag gcttcgcgcc agcagtcccg ccaggaactg
     4261 cattcgctcc aggcgccttc ccagcatgag gaggcgccat ccgacatcct gcgtcatgtc
     4321 gtccaaggcg aagcccgcga gcgcggccag cgataacagc aaggcgtcca gcgtctcgcg
     4381 cggatccctc cggcgttcgg cggcttcttg aaattgacgc tgcaggacgc tgatggcgcg
     4441 ccaattctcg gtcgacagcc ggctgcgcgt ctgcgccgcg caccattgca aacgctg
//
LOCUS       DATGEM010000090         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1571724, whole genome shotgun sequence.
ACCESSION   DATGEM010000090 DATGEM010000000
VERSION     DATGEM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571724"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VKQ27_02940"
     CDS             complement(<1..686)
                     /locus_tag="VKQ27_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HLY87920.1"
                     /translation="MSPANTPLLDRVRIPADLRNFSPEQLRQLADELRTETIDAVSVT
                     GGHLGASLGVVELTVAIHAVFDTPHDRLLWDVGHQAYPHKILTGRRDRIRTLRQGGGL
                     SGFTRRAESEYDPFGAAHSSTSISAGLGMAVARDLKGDTRNVIAVIGDGAMSAGMAYE
                     AMNNAGALHSRLIVILNDNDMSIAPPVGAMSAYLSRLMSSRSFLSLRELGAKMARRFP
                     RGIERTLGRA"
     gene            complement(707..1606)
                     /locus_tag="VKQ27_02945"
     CDS             complement(707..1606)
                     /locus_tag="VKQ27_02945"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HLY87921.1"
                     /translation="MSLVETELSAALSRVAGVVEAALEALLPPVQGAEGRLAEAMRYA
                     TLGGGKRMRAFLVMQTSALFSVSETCAARVAASVEMLHAYSLVHDDLPAMDDDDLRRG
                     KPSTHKAFDEATAILAGDSLQTRAFEVLAEPDTHSDPQARCELVMGLGAASGARGMAG
                     GQMIDMLAEGQTLDGPAVTRLQALKTGRLIQFSAEAGAILGRAPLAQRHLLAAYGRDL
                     GAAFQIADDLLDAEGSTEETGKTAGKDAAAGKATMVAVLGLERARAQAELLARQAAGH
                     LDGFGERGALLRSLAAYVVTRRN"
     gene            1874..>2236
                     /gene="ispH"
                     /locus_tag="VKQ27_02950"
                     /gene_synonym="lytB"
     CDS             1874..>2236
                     /gene="ispH"
                     /locus_tag="VKQ27_02950"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421330.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLY87922.1"
                     /translation="MIRVVLAQPRGFCAGVERAIEIVERALKKFGPPIYVRHEIVHNR
                     HVVEDLRTRGAVFVDELDEIPPGAMTIFSAHGVAKRVEELAAERQLPVIDATCPLVAK
                     VHNEGRRYAAQGREIVLVG"
BASE COUNT          392 a          794 c          674 g          376 t
ORIGIN      
        1 tcggcgcgcc cgagcgtgcg ttctatgccg cgcggaaaac gccgcgccat tttggccccg
       61 agttcccgca acgacaggaa gctgcgtgac gacatcagcc gcgacagata ggcgctcatc
      121 gcccccaccg ggggcgcgat cgacatgtcg ttgtcgttca ggatgacgat cagccgggag
      181 tgcagcgcgc cggcgttgtt catcgcctcg taggccatgc cggcgctcat cgcaccgtcg
      241 ccgatcaccg cgatgacgtt cctggtgtcg cccttcaggt cgcgcgccac cgccatgccc
      301 aggcccgcgg agatcgacgt ggacgaatgc gccgcgccaa acgggtcgta ttcgctctcc
      361 gcccggcggg tgaagccgga caggccacca ccttggcgca acgtgcggat ccggtcgcgc
      421 cgcccggtaa ggatcttatg cgggtaggct tggtgaccca cgtcccagag cagccggtcg
      481 tgcggcgtgt cgaacacggc gtggattgcc accgtcaact cgaccacgcc caaagaggct
      541 ccgaggtgac cgccagtgac cgataccgcg tcgatcgtct ctgtccgcag ttcgtccgca
      601 agctgccgca actgctccgg tgagaaattg cgcagatcgg ccggaatccg gacccgatcc
      661 agcagcggtg tgttggcagg actcatcttc actacacccc gatgttctaa ttccgccgtg
      721 ttacgacata ggctgccaga gaccgcagca gcgcaccgcg ctcgccgaag ccgtccaggt
      781 gaccggctgc ctgacgcgca agcagttcag cctgcgcccg tgctctctct aaccccagga
      841 ccgcgaccat agttgctttg ccagcggcgg catctttacc ggcggtttta ccggtttcct
      901 ccgtactccc ctcggcgtcc agtaagtcat ccgcgatctg gaaagccgcg cccaaatccc
      961 gcccatacgc cgccagcaaa tgccgctgcg ccaacggggc ccgccccaag atcgcgcccg
     1021 cctcggcgct gaactggatc agccgtccgg tcttcagcgc ctgaagccgc gtcactgccg
     1081 gtccgtcaag cgtctggccc tctgccagca tatcaatcat ctgcccgcca gccatcccac
     1141 gggcgcccga ggccgctccc aaccccatga ccagctcgca gcgtgcctgt gggtctgaat
     1201 gcgtgtctgg ctccgccagt acctcgaatg ctcgggtctg cagcgaatcg cccgccagga
     1261 tcgctgtcgc ctcatcgaac gccttgtgcg tcgacggctt gccccggcgt agatcatcgt
     1321 cgtccatcgc cggcaggtca tcatgaacca gcgaataagc gtgaagcatc tcaaccgacg
     1381 ccgctacccg cgccgcacag gtttcagaca cagagaacag ggcagaggtc tgcatcacca
     1441 ggaacgccct catccgcttg ccgccaccaa gcgtcgcata gcgcatcgct tcggccagac
     1501 gcccttccgc cccttgcaca ggcggcagca gcgcctccag cgcagcctcg acgaccccag
     1561 cgacacgcga caaggcggca gacagctccg tttcaaccaa gctcatcacg ccggccttcc
     1621 gatgccagtc ctgagcctct gaatctgtca caatccgacc aaatgggaat gctcgcaccc
     1681 ctgtggttgc acgattcaca cgcacgtgtg gctcggctgc ctcgaatccg ttactgcctt
     1741 accgcaacca tggcgggcag ggaaggtcag ggctttgccc cgaacctgcc cggcgaaagc
     1801 gggggcggac caccggcctt tcttgccggg ccgggggtgg catgccatat gccggacaat
     1861 tcgggagagg cccatgatcc gcgttgtact cgcccagccc cgcggcttct gcgccggtgt
     1921 cgagcgggca atagagatcg tcgagcgggc gctgaagaag ttcggtcccc ccatctacgt
     1981 tcgtcacgaa attgtgcaca accggcacgt cgtggaggac ctgcggaccc gcggcgcggt
     2041 gttcgtcgat gagctggacg agatcccgcc gggcgctatg accatcttct cagcccatgg
     2101 ggtcgccaag cgggtggagg aactggcggc ggagcggcag cttcccgtca tcgacgcaac
     2161 ctgcccgttg gtggccaagg ttcacaacga ggggcggcgc tacgccgccc aggggcgcga
     2221 gatcgtcctg gtggga
//
LOCUS       DATGEM010000091         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_197352, whole genome shotgun sequence.
ACCESSION   DATGEM010000091 DATGEM010000000
VERSION     DATGEM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197352"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VKQ27_02955"
     CDS             <1..1138
                     /locus_tag="VKQ27_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HLY87923.1"
                     /translation="ARPAVSATAPPPIVPFHGANQAGIVTQQQSHTYFLAFDLLTAMR
                     DDVTQLLRAWTDAAARMTSGAPASAPDSSEALGLAPSRLTVTFGLGAGLFQKDDHDRY
                     GLAARRPAALIDLPTFNGDQLVPERTGGDLSIQACADDPQVAFHAARQLARLAGSAAR
                     VRWAQTGFLSRPAGGGTPRNLLGFKDGTQTPQEVAKVVWVDAEGPDWMRGGSYVVVRR
                     IRMALEHWDHTDVDFQEQTIGRHKMSGAPLGLRDEFAPLGLDRMDKDENPVIPETAHV
                     RLANAASNGGAEILRRGYSYNDGVNFTAERWPPWRQSMEYDAGLFFLCYQRDPRAGFV
                     RIFENMARLDMLNQYVTHSGGGLFACPGGVGPGEFIGHKLFTSS"
     gene            1231..>2484
                     /locus_tag="VKQ27_02960"
     CDS             1231..>2484
                     /locus_tag="VKQ27_02960"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY87924.1"
                     /translation="MSRGNATTVTSFSRASRTAFLALALLLILGDALLAILLLDDERR
                     ADAKVERALQVTMRLQRIEDLAIRCGRDQRAYRISGDVVRLEAFRQEAAQLRPELASL
                     GKLVADTGHVAAQQADLVRLIDEDLSALASTLTPISTHFDDRTLPPELTDSIRRSTAI
                     VAGVDTMASNEAIMLRAQLAAIKTRNMIMLGTVGIGAISGVILLAIILGLLRRETQRL
                     AAASTGALEESEQRFRRIFEESPLGIVLAQRDDLEVVQANPAFCRMIAYPTEQIVGRT
                     ITNMTHINDRDLLLRASTRAGEPGHEVEVRFVTGSAVLAWAHISLSQLGPSDSRPNLL
                     LALVQDTTRERRVEAELRQAQKMEAIGQLTGGIAHDFNNLLGVIIGNAEFLLDALRED
                     LSNNHLAQEILDSALSGADLTRRLLA"
BASE COUNT          447 a          829 c          772 g          436 t
ORIGIN      
        1 cgcccgcccc gccgtatcag ccacagcccc tccaccgatc gtgccgttcc acggtgccaa
       61 tcaggcaggc atcgtcacgc agcaacaaag ccacacctac tttctggcgt tcgatctgct
      121 gacggccatg cgtgacgacg tgacccagtt gctgcgtgcc tggacggacg cggcggcgag
      181 gatgacctcc ggcgcgcctg cttccgcgcc agactcgtcc gaggcgctcg gccttgcgcc
      241 ctcccgcctc accgtgacct tcggcttggg cgccggcctg tttcagaagg atgatcacga
      301 tcgctacggc cttgccgccc ggcgcccggc cgccctgatc gacctcccta catttaacgg
      361 cgaccagctt gttccggaaa ggacgggcgg cgatctgtcc attcaggcct gcgccgacga
      421 tccgcaggtg gcgttccatg ccgcccggca actcgcccga ttggcgggat cagcggcccg
      481 ggttcgctgg gcgcaaaccg gcttcctgtc gcgtccggcc ggcggcggca caccgcgtaa
      541 tctgctcggc ttcaaggacg gcacgcagac gccccaggag gtggccaagg tcgtctgggt
      601 ggacgccgaa ggtccggact ggatgcgcgg gggcagttat gtcgtggtgc gtcggatccg
      661 tatggcgctg gagcactggg accataccga tgtcgacttc caggaacaga cgatcggccg
      721 gcacaagatg tccggagcgc cgcttggcct gagggacgag tttgcaccgc tcggcctgga
      781 tcgtatggac aaagatgaga acccggtgat tccggagacc gcgcacgtcc ggctcgcgaa
      841 cgccgccagc aacggtggcg cagagatcct gcggcgcggg tattcctaca acgacggcgt
      901 caacttcacc gcggaacgct ggcccccctg gcgccagagc atggagtacg atgccgggtt
      961 gttcttcctc tgttaccagc gcgatccgcg cgccggcttc gtccgtattt tcgagaatat
     1021 ggccaggctc gacatgctga accaatacgt cacccactct ggcggcggac tgttcgcctg
     1081 cccaggcggg gtgggtccag gcgagttcat cggccacaaa ttgttcacct cctcctgagc
     1141 ggccggcccg gcttccgcgt tcaccgctac tgcatcagca tcggtagaca tgatacgcat
     1201 gtctgcaacc gagagtaggc tgcctgtacg atgagccgag gcaatgcgac gaccgtcacg
     1261 tccttcagcc gtgcatcgcg gacggcattc ctggcgctcg cattactgct gatactgggc
     1321 gatgccctat tggcgatcct gttgctggac gacgagcgcc gcgccgacgc caaggtcgaa
     1381 cgcgcgctgc aagtgacgat gcggctgcaa cggatcgagg acctcgcgat ccgctgcggc
     1441 cgcgaccagc gcgcctatcg catctcgggt gacgtcgtca ggctcgaggc cttccgccag
     1501 gaagcagcgc aattgcgccc ggaactggcg agcctcggca aactggtcgc agacaccggg
     1561 cacgtagcag cgcaacaggc cgatcttgtc aggctcatcg acgaggacct gagcgcattg
     1621 gcttccacac tcacgccgat atcaacccat tttgatgaca ggacactgcc gccggaactc
     1681 accgacagca ttcgtcgttc cacggccatc gtggccgggg tcgacacaat ggcgtctaac
     1741 gaggccatca tgctccgcgc gcaactggcc gccatcaaga cacgcaatat gatcatgctg
     1801 ggcactgtgg ggatcggtgc cattagcggc gtcatcctgc tcgccatcat actgggcttg
     1861 ttgcgccgcg agacacagcg tctcgccgcg gcgagcaccg gcgccctgga ggaaagcgag
     1921 cagcgcttcc gccggatttt cgaggaaagc ccacttggca tcgtgctggc ccaacgcgac
     1981 gacctggaag tcgtgcaggc caacccggca ttctgccgca tgattgccta tccgaccgag
     2041 cagatcgtcg gccgcaccat cactaatatg acgcatatca acgaccgcga tttgctgctg
     2101 cgggccagca cgcgtgccgg ggagcccggt cacgaagtgg aagttcgctt cgtgaccggc
     2161 tccgcggtgc tggcctgggc acatatcagc ctgtcgcaac tcggtccgtc ggacagccga
     2221 cccaatctgc tgcttgccct cgtccaggac accacgcgcg agagacgggt cgaggctgaa
     2281 ctgagacagg cgcagaagat ggaggccatt ggccaactca ccgggggcat tgcgcacgac
     2341 ttcaacaacc tgcttggcgt catcatcggc aatgccgaat tcctgctcga tgcgctgcgg
     2401 gaggatttgt ccaacaacca tctcgcccag gagatcctgg acagtgcgct gagcggtgca
     2461 gatctcacgc gtcggttgct tgcg
//
LOCUS       DATGEM010000092         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_428308, whole genome shotgun sequence.
ACCESSION   DATGEM010000092 DATGEM010000000
VERSION     DATGEM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428308"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..896
                     /locus_tag="VKQ27_02965"
     CDS             96..896
                     /locus_tag="VKQ27_02965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLY87925.1"
                     /translation="MRVPVLLFALFLAVQARAQSPAAPAMLPPNAANSIESEVARLRL
                     ALAREVPRLIRDRPARAAEWIALARAQLAAGGDKIERPQLLVAVDRNPRVQQMRILLA
                     RPDGPWEDLGGAKVSTGQAHRVGYFITPRGVFKHTDVILDWRAEGTFNENHIRGLGLK
                     GMRVWDFGWQSALKGWLHTGERGQIRLLMHATDPANLARRIGRPASEGCVRVPADVNR
                     FMDRHGVLDVDYQQVATLDVFYRAILSNDANPTPLAGDKLVIVDSAEE"
     gene            complement(932..1223)
                     /locus_tag="VKQ27_02970"
                     /pseudo
     CDS             complement(932..1223)
                     /locus_tag="VKQ27_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612229.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1301..1576
                     /locus_tag="VKQ27_02975"
                     /pseudo
     CDS             1301..1576
                     /locus_tag="VKQ27_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1839..2081)
                     /locus_tag="VKQ27_02980"
     CDS             complement(1839..2081)
                     /locus_tag="VKQ27_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87926.1"
                     /translation="MKIERPDSIPLGMPAAKALQMATAAMRRRYVLPETIEHPVVRAE
                     CFATGLSDPGLRFGISPARWTPCAEGHGKPFCSRQW"
BASE COUNT          397 a          733 c          644 g          392 t
ORIGIN      
        1 gccgtcgcga aggacgcagg atgttcgcgt agcgccgtcg cgttgcgctg atccgtgcta
       61 aaagggcgca tcgtcctgga ggatttcaac cgccgatgcg cgtcccggtc ctgctctttg
      121 ccttgtttct tgccgtccag gcccgtgccc aatcgccggc cgcgccggcg atgctgccgc
      181 cgaatgccgc gaacagcatc gaaagcgaag tcgcccgcct gcgcctcgcg ctggcccgcg
      241 aggtgccgcg cctcattcgc gaccggccgg cgcgcgccgc cgaatggatc gcgctcgccc
      301 gagcccagct cgccgccggc ggcgacaaaa tcgagcggcc gcagctcctg gtggcggtcg
      361 accgcaatcc gcgggtgcag cagatgcgca ttctgctggc gcgccccgac gggccgtggg
      421 aggacctcgg cggcgccaag gtgtccaccg ggcaggcgca tcgcgtcggc tatttcatca
      481 ccccgcgcgg cgtcttcaag cataccgacg tgatcctcga ctggcgcgcc gagggcacct
      541 tcaacgagaa ccatatccgc ggcctcgggc tgaagggcat gcgggtgtgg gatttcggct
      601 ggcaatcggc gctcaagggt tggctgcata ctggcgaacg cggccagatc cgcctcctga
      661 tgcacgccac cgatccggcc aacctggccc ggcggatcgg tcgccccgcc tcagagggct
      721 gcgtgcgggt gcctgcagac gtgaaccgtt tcatggaccg ccacggcgtg ctggatgtcg
      781 actaccagca ggtggccacg ctcgatgtgt tctatcgcgc catcctgtcc aatgatgcca
      841 acccgacgcc gctcgctggg gacaagctgg tgatcgtcga ttcagcggaa gaataacctc
      901 gaagccatag gcgtaatcaa caccgacacg gcttccaagc tgccagctcg gtcgggtaca
      961 cgccgcgctc gcgacaccat gcattgcggg tggcttcatc ccatcgcggc agtggtgatc
     1021 actgtctcca gtcgcgccgc ggcggtccaa gtctgaccac cgcccgtccc aatggctgga
     1081 ttgaccaatg cctctgaccg ccaccgttcc agcgtcgcca cactgatgcc aaccttctgc
     1141 gacacctcct caaccgccgc actctccggc ggcatcaatc gcgcaactac ccgctctttg
     1201 aacttctgtc cgtatctcgc cacgtccgtc ttccagcgcc cccagggcca tcatcctatc
     1261 cgtgcgacaa ccatcctgac acaggagact ccagcgtctc gttgatcagc aacagcggga
     1321 accgctcgcg gcagtgttcg gcgcaggttc tgcatcgtcg tcagatggtc agttcttccg
     1381 ggccagtggg ttcggtcgca acgccagcag cctcaacgcc cactatggtg acgattccgg
     1441 tgtcaagttc tacacccacc tctccgaccg atatgcaccg ttccacacca agttgatcgc
     1501 ggagaccgtc actggcagct cgccagctcg caaacgctgc acgcgccggc gagataaaca
     1561 cggtcaattg ccgtgacatc ggcgccctga cgtcgactgg cgtcaaaaga gaatgcggac
     1621 cgtctgggat aacgaaccag agccgaagaa gcaccggcct gccatatcga ggtcactgcg
     1681 gcgaatggcg tgaggttcac cgtccactta tgggctgacg gctctgtgtc agtcacaggc
     1741 taacgcgtaa gaatggtcgc cattaggcgt caaccagctc taatgcccgg gtctctcggg
     1801 gcacgtaacg ccgaggctga tcgaagaaac acggtgcatc accattgcct gctgcagaac
     1861 ggtttgccgt ggccttcggc gcagggagtc catctggccg gtgagatgcc aaaccgtagg
     1921 cctggatcag ataggccagt tgcaaaacat tccgccctga ccacgggatg ttctatggtt
     1981 tcgggcagca catagcggcg acgcatggcc gcggtggcca tctggagtgc cttcgccgca
     2041 ggcattccga gcggaatgct gtcggggcgc tcgattttca tgacgccttc cctcgatgaa
     2101 acgtaatcac acacgaataa gacaacgccg cattgatgca gatcagtggg tcagccctac
     2161 ttccct
//
LOCUS       DATGEM010000093         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_428206, whole genome shotgun sequence.
ACCESSION   DATGEM010000093 DATGEM010000000
VERSION     DATGEM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428206"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VKQ27_02985"
     CDS             complement(<1..1456)
                     /locus_tag="VKQ27_02985"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HLY87927.1"
                     /translation="MSLRTLFRPVAVPAGKAFVRPLAALLLMSTALPALAGAATPTDR
                     LLAAAEDAGPVDPTTPMTATVWLAGSNQAQLDGYVADAYNPKSLNYHRWLNASQLAAF
                     GPTAQTVATMRQSLAAQGLKVESVFPDGAGIKVSGAAAKMQAAFGTSIHVKQSGGVNY
                     FANVTTPRYQGAHPELVAAVSGLSSAEMAPFALRQLDVSTGQPMPAVPMAQNGSYPLS
                     NFTTNCFGPTITATMTGITRGIGRLSHVTGIYSGQSYLNPATIFRPQCGYTAQQVVAH
                     YGMNTVHANGFTGKGQTIVIVDAYGSPTIAADVNTFSQAMGLPAMTSTSLQIVYPDGP
                     PPAGANGWQDETSLDVEWAHALAPDANIVLVVAPNASNGELAYAIHYAVAHQLGNVIS
                     NSWGGPEAGNSAAVAQMFNSVFKTAAAAGISVNVATGDQGDFGVGSPVGAASIPADSP
                     YATGVGGTSINVPSDNGLVDTAWGLTWSQLGYINA"
     gene            complement(2491..3102)
                     /locus_tag="VKQ27_02990"
     CDS             complement(2491..3102)
                     /locus_tag="VKQ27_02990"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY87928.1"
                     /translation="MDAAQRLFLVSGYDGVNLDQIGTAASVSRQTVYNQFGSKEAVFR
                     AVVQRHWDTIRAETGSSFAMHPDTATDPAHVLRRFAAALVRFMSETDQIAFTRLVIAE
                     SRRVPWIAEEFYRAGKEPIQKAFTGALALMTERGLIRCASPELAARQFMGLIQEFIIW
                     PRVMAIDEAVAGLPSSELVIEEAVSTFLARYARSESRPAAREA"
     gene            complement(3592..3743)
                     /locus_tag="VKQ27_02995"
                     /pseudo
     CDS             complement(3592..3743)
                     /locus_tag="VKQ27_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(3911..4546)
                     /locus_tag="VKQ27_03000"
     CDS             complement(3911..4546)
                     /locus_tag="VKQ27_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY87929.1"
                     /translation="MTSKNDSGSSAPILGAAQQLFLASGYDGLDLDLVGKAAGVSRQT
                     VYNQFGSKEAIFRAMVQRHWDTIHFEAASSFAIQTEMATDPAHVLRHFATTLLRFVTE
                     TDQVAFTRLVIAESRRLPWIAAEFYRAGKEPILKAFAGALALMTEKGLIRCTYPELAA
                     DQFMGLLQEFITWPRVMAIDEAAAADLPPTNSIIEEAISMFLARYARPATR"
     gene            complement(5136..>5617)
                     /locus_tag="VKQ27_03005"
     CDS             complement(5136..>5617)
                     /locus_tag="VKQ27_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY87930.1"
                     /translation="FGFVPSLQANMAESPELLAGYSALWDLFSKSTLTSHEQQVVYLT
                     SNFENECHYCMAGHSTLAKMLKMDPAVIEALRNGTPLPDAKLEALHRFTTLVVRNRGW
                     VSDAETDAFLTAGYTRRNVLEVVLGVATKVMSNYTNHIVHSELDSFMHGNEWAKPAA"
BASE COUNT          995 a         1737 c         1797 g         1088 t
ORIGIN      
        1 gtgcgttgat gtacccaagt tgcgaccagg taagccccca ggccgtgtcc acaaggccat
       61 tgtcgctcgg cacgttgatg ctggtgccgc cgacgccggt ggcgtagggc gagtcggccg
      121 ggatcgacgc agcgccgacc gggctgccga caccaaagtc gccctgatcg ccggtcgcga
      181 cattgacaga gatcccggcc gcggccgcgg tcttgaacac gctgttgaac atctgcgcga
      241 cagcggcact gttccccgct tccggaccgc cccagctgtt cgagatcaca ttgccgagct
      301 gatgcgcgac cgcgtaatgg atggcatagg cgagctcgcc attgctggcg ttgggcgcca
      361 cgaccaggac gatattggcg tccggtgcca gcgcatgcgc ccactcgacg tcgagcgagg
      421 tctcgtcctg ccatccgttg gccccggcgg gcggcggccc gtccgggtag acgatctgaa
      481 ggctggtgct ggtcatcgcc ggcaagccca tggcctgcga gaaggtgttg acgtcggccg
      541 cgatcgtcgg cgagccgtag gcatcgacga tgacgatggt ctggcccttg ccggtgaagc
      601 cgttggcatg gaccgtgttc attccataat gggcgacgac ctgttgggcg gtatagccgc
      661 actgcggacg gaagatcgtt gcgggattga gatagctctg ccccgagtag atgccagtga
      721 cgtgggaaag gcggccgatt ccccgggtta tgccggtcat cgtcgccgtg atagtcgggc
      781 cgaagcaatt ggtcgtgaag ttggaaagcg gatagctccc gttctgggcc atcggtacag
      841 ccggcattgg ctgaccggtc gatacatcga gctgtcggag cgcgaagggc gccatctcgg
      901 cactgctcaa gccggacact gcggcgacca gttcggggtg ggcgccctga tagcgcggcg
      961 tcgtgacgtt ggcgaagtag ttcacgccac ccgactgctt cacgtggatg ctggtgccga
     1021 aggcggcctg catcttcgcc gctgcgcccg acaccttgat cccggcgccg tcagggaaca
     1081 ccgattcgac cttcaacccc tgggcggcaa gagactggcg catcgtcgcc acggtctgcg
     1141 ccgtgggtcc aaaggccgcg agctggctgg cgttcagcca gcgatgatag ttcagcgact
     1201 tcggattgta ggcgtctgcg acatagccgt ccagctgggc ctggttgctg cccgcgagcc
     1261 acactgtggc cgtcatcggc gtcgtcggat cgaccggccc cgcatcctcg gccgcggcca
     1321 gcaggcgatc cgtcggcgtc gccgcgccgg cgagggccgg cagcgcagtg ctcatcagga
     1381 gcagcgccgc cagcgggcgc acgaacgcct tcccggccgg cactgccacc ggccgaaaca
     1441 gagtacggag actcattcca atcaatcctc tttggagata gaagaacgga cttctagatg
     1501 ccggtgagat tctctcgcga ttcgcaaagg acatggctgt gcgcgatcgg tccgtgcgca
     1561 gcactgccga ttgatcgcct cagcgtttat tcggttggat tctggtaatt ttagaaatac
     1621 ttcctagccc tgtagttttt attcttcttc cggcccgatg atggcgagag cttcacgttg
     1681 attgatgctt cccgcacctt tcgcccgacc gccgcgcgat gccgattggt tacaggggca
     1741 accttttgca cgattgtact gtattgtaaa gtcaaaattg tgacaccccc cttttccact
     1801 cgtcacctgt ctcggaaatt tgatctctgg aaccggggcc gttccgagtt ggcgttttcc
     1861 gtcttaaatt tcccgagcgt ccccgcgctc cgccgcacga ttgtggccgc actgcccgtt
     1921 ggtctcccag gcagggatcg gcttcctcga cccgggcggc aacgcgttcg acgcggcggt
     1981 ggcggcgcgc aggacatggt agggccgttt attttgggct tcgcggcagc gttgacctat
     2041 ctcgggcctg gggtttccgg tgcaaagcgg tcggccattc gagagaaggc ggttaacgat
     2101 tgcggtcgaa gccggccgcg ttcgggcggg gctaattggg ctccaaattg gcgatctcag
     2161 ggacggcgat cgtagccccg cgacgagagc gaggggacgg ggtgcccgcc gagtgattgc
     2221 caatgcgcaa aggccgagaa atacttcgtc cgagcaggcg cgcggggcag cgggcgtgtg
     2281 attgcccggt cgttcggcat tggccgcagg tcagtgagcg aggagctgcg gcgtgccgcg
     2341 gttatcggca tcacccgacc ggtaccggcc agcatcgacg acgcctggcc gcaggaatgg
     2401 gttagcggtg gcgagaacga aggggcgatc cacaccgata ccaaccggca tcggtatgac
     2461 gatcaggccc ctgcgcggcc caacgtggtc tcaggcctcc cttgcggccg gcctactctc
     2521 cgacctcgcg tatcgtgcca agaatgtcga aacggcctct tcgatgacca attcgctgga
     2581 gggcaagccc gccaccgcct cgtcgatcgc catgacgcgg ggccaaatga taaattcctg
     2641 aatcaggccc ataaactggc gagcggcgag ttccggagag gcgcagcgga tcaggcccct
     2701 ttccgtcatc agagccaggg caccggtgaa ggccttctgg atcggctcct tgccggcgcg
     2761 atagaactct tcggcgatcc agggcactcg gcgggattcg gcgatgacca ggcgggtgaa
     2821 ggcgatctgg tcggtctccg acatgaaacg caccagggcc gccgcgaaac gccgtagcac
     2881 atgggctggg tcggtggcag tatccgggtg catcgcgaag gacgaaccgg tttcggcgcg
     2941 gatcgtgtcc cagtggcgct gaaccaccgc gcgaaacacc gcctccttgc tgccaaactg
     3001 gttgtagacc gtttggcgcg acacgctggc ggccgtaccg atctggtcca gatttacccc
     3061 gtcatagccc gataccaaaa atagcctttg tgcggcatcc aagatcgctg ccgaagtgtc
     3121 gttgtcggat ccggctcttc cttcggcttt ccgcgtcgat cgtaccaccg ctcagtaccc
     3181 tcgcttccgg aacatttact ttacagtaaa gtatagtatt tatatcacac ccaagaacaa
     3241 tgcgcatcga tccgccgcta aggcaacgac cgcggattcg ccgatggagt ctcgccatgt
     3301 cgaacgatcc ctggccccgc cggctgcaat cgtcggtccg tccttgccct ggactacgca
     3361 gacgcaaacc gaccaatcgg gcggccgatc agtcgcgcct gccagccgat gtttcggggg
     3421 cctgggccgg tccgcggagc acctgcacct gtctgcgcgg tttcgtggat ctttcccgca
     3481 actcgccctc aggcgctctg gaaaggggca gcggatgaga gggcgctgga ggttaaatgt
     3541 gttgtgacac ggcggcgtgg ccggggagga acctgtgggc ggagcctgtg acttgaatct
     3601 ttgccactta cgctctcgtc ggcgaaccgg caggtgagaa ttttcggctc ggttattttc
     3661 tcgcgggccg ccagccaagt ggcggtcgga aagcccagtt cccgggcggc ggcgccatgg
     3721 gccccgagct tgtcgatcac gactcagccc gcatatcggg ctgcattccc aaccagacct
     3781 ttctcgtcgg gacaggctcg ggcgtcttca atcgtatcgg cccaggccag cggtcataaa
     3841 cgcgttttgc gagccggaaa gaatatcagg ccatgcgcga ccgaatatga cttcaggcaa
     3901 ccactgcgcc tcacctagtc gccggcctcg cgtaccgcgc taagaacatc gaaatggcct
     3961 cttcgatgat cgagttggtg ggcggcaggt ccgctgcagc cgcctcgtcg atcgccatga
     4021 cgcgaggcca ggtgatgaat tcctgaagca ggcccatgaa ctggtcggcg gcgagttctg
     4081 gataggtgca tcggatcagt cccttttccg tcatcagagc cagagcaccc gcgaatgcct
     4141 tcaggatcgg ctccttgcca gcgcgataga attccgcggc gatccaggga agtcggcggg
     4201 attcggcgat gaccaagcgg gtgaaggcga cctgatcggt ctccgttacg aagcgcagca
     4261 gtgtcgttgc gaaatgtcgc agcacatgcg ccgggtcggt ggccatctcc gtctgtatgg
     4321 cgaaggacga cgcggcttcg aagtggatcg tgtcccagtg gcgctgaacc atcgcgcgaa
     4381 aaatcgcctc tttgctgccg aactgattgt agaccgtctg gcgtgacacg ccagcggcct
     4441 tgccgaccag gtccaggtcc aagccgtcgt aaccggatgc aagaaacagc tgttgcgcgg
     4501 cacccaagat cggcgccgaa gagccgctgt cgttttttga cgtcatgcga cctgctccca
     4561 ttgatttgct cgtcccgatg cttcggcttc cgcggtacca gggactcacc ctggatctct
     4621 ccacccggcg gcacgctgca ccggctgtcc ctcgtcgctc tggatggctg ataatcgggt
     4681 gatcccgtag atggttcgtt gcagaacacc agagcagtcg ccggaccggc cgagtgaccg
     4741 aacgtctcaa tttcatgtcc tatctgtaaa tcggcccggg ttccgatgcc ggtcgtgacg
     4801 tcgcgccact tcactgccat tcaccggctt gccggacatc gttggtcgca cgcgtcatca
     4861 cagcgtcatg ctttcgacgc cgcgcgcgga gtagcggacg aacgaggcct cccgagtacc
     4921 gaatccgttg caagaactct gacgggcggc atcggccaga tccagcagcc ggcggccgga
     4981 acggccctga ggcgcggcct ggcccgaagg tagccgggcc gctacctcat caggcgtcac
     5041 ctcctgcgag cacgaatatg cgcgtgccgt ttctttggcc catctccgcc tgacattaac
     5101 gaagggatct cttccgcgtt ggtttccgaa gccgctcagg cggccggctt ggcccactcg
     5161 ttgccatgca tgaagctgtc gagctcgctg tggacgatgt gattggtgta gttgctcatc
     5221 accttggtcg cgacgccgag gacgacttcg aggacgttgc gacgggtgta gcccgcagtg
     5281 aggaaggcgt ccgtctcggc atcggaaacc cagccgcggt tgcgcacgac cagggtcgtg
     5341 aaacgatgca gcgcctcgag cttggcgtcg ggcagcggcg tgccgttgcg cagggcttcg
     5401 atcacggccg ggtccatctt cagcatcttg gccagcgtcg agtggccggc catgcagtag
     5461 tggcactcgt tctcgaaatt cgaggtcaga taaacgacct gctgctcatg ggacgtcagg
     5521 gtcgatttcg agaacagatc ccagagggcc gaatatccgg caagcagttc cggggactcc
     5581 gccatgttcg cctgtaggct cgggacgaaa ccaaacg
//
LOCUS       DATGEM010000094         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_653528, whole genome shotgun sequence.
ACCESSION   DATGEM010000094 DATGEM010000000
VERSION     DATGEM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653528"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VKQ27_03010"
     CDS             complement(<1..271)
                     /locus_tag="VKQ27_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HLY87931.1"
                     /translation="MSKHVVATVAEIPPGQRKLVTVRGRSIAVFNLGGEFFGLFNRCP
                     HQGGPMCEGILTGLIESDEPGHYKYSRKGEIIRCPWHGWEFDVRTG"
     gene            398..1489
                     /locus_tag="VKQ27_03015"
     CDS             398..1489
                     /locus_tag="VKQ27_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY87932.1"
                     /translation="MRGTRFGMAALAASLLCGQANADTIKFGGILPLTGPGALIGTAE
                     MHGIQFAVDQANAKGGVHGNKIEVQFEDNQAKPDQSVLSFNKLTDLEHVSVIFTGYSG
                     PTLAMAPLATRKKVLLVNAGAQADALAKASPYLINTLPTIGDEIAVLSKWLVGEGKKQ
                     GAIMFENDAAGMSGRDDYLRSFPDAGGKVLAQEATQFGQTDFRPALLKLADAKPDVML
                     VAITAGLLQMAQEYHQLGLKFTVAGTTFFADPATIADPSSAGFVHTQLRIDAPPELAA
                     QFKAKYGVDMEFFAKQYYSAAQIVLTTLDKVLSEKQPVTGENMHDTLLKIGKFQGLIP
                     LEFKTNTASVPIDINVMRDGKDVAIKHIQ"
     gene            1500..2363
                     /locus_tag="VKQ27_03020"
     CDS             1500..2363
                     /locus_tag="VKQ27_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY87933.1"
                     /translation="MLALQLLATGVVTGCALGVVAISFALVYATTKIFHVAHAGIYTL
                     AGYLAWSLVLWGAPLIVAVVVSIAICTAFGALIQSQLYARLERRRATHLVVLIASLGA
                     LAVIQNVIAGVYTPNILQFPLAWSGEVLPLGSVRLTYVQLLTIAVSLAAYVGTMWFAH
                     HTILGKRIRAVASNPMLADITRLQPQSVYIYVIAIASALVCLPGILVPMDLGLQPYNG
                     VTPLLTATIAMIAGGVGSITGAFVLSVAIAVLQNLSLLVMPGEWSIGVTFFIFVIFML
                     FRPTGLFAAAR"
     gene            2363..3229
                     /locus_tag="VKQ27_03025"
     CDS             2363..3229
                     /locus_tag="VKQ27_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY87934.1"
                     /translation="MDFLYSYIVLAEIYVLLGLSTNLLVGIAGIFSVSQAAVFGVGAY
                     LVAHFLMAQDALPFVPSLAIAAACCVALNILVTLPSLRVAGDYFVVTSFGIQLLATAV
                     FTNWTAGTGGANGLPGIPPPDILGLQIESTQQYVVLCTVAAGLGCLCFFLVMRAPFGR
                     LLRAIREDELAVAAAGKNVLRAKVSAAALAGAFAGIAGGLYATFLSFIDPSSFDLDAS
                     ILLLTMVVVGGARTLAGSIIGPFLLLALPQVLTLIDIPTTIAASARQLIYGVLLIAFM
                     LFRPQGLAGERL"
     gene            3226..>3589
                     /locus_tag="VKQ27_03030"
     CDS             3226..>3589
                     /locus_tag="VKQ27_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY87935.1"
                     /translation="MTAYLQAENLDHHFGGLRALRECTFAINQGRITCLVGPNGAGKT
                     TIFNAITGFLKPDEGSVTFKGRKLDGLRPQAIVHAGIARTFQNLRLFTDLTALDNVMV
                     GIGGQFGEEPVGAIFRPLH"
BASE COUNT          602 a         1157 c         1104 g          726 t
ORIGIN      
        1 ggccggtacg cacgtcgaat tcccagccgt gccacggaca gcggatgatc tcgcctttgc
       61 gcgaatactt gtagtggccg ggctcgtcgg attcgatcag gccggtgagg atgccttcgc
      121 acatcgggcc gccctgatgc gggcagcggt tgaacaggcc gaagaactcc ccgcccaggt
      181 tgaacaccgc gatagaacgg ccgcgcaccg tcaccagctt acgttgtcct ggcggaatct
      241 ccgcaaccgt cgccaccacg tgcttgctca tgcgataacg tcccatctgc cactcgaacg
      301 ctaccgcccg ccgcactgcc ctgcaacggc ttgaccccct gagatgcgct ccctagcttg
      361 ccgcgctcga cgtccggaaa gcgggggaaa catcacaatg cgtgggactc gtttcggcat
      421 ggcagctttg gcggccagcc ttctatgtgg ccaggccaat gccgatacca tcaagttcgg
      481 cggtatcctg ccgctgactg gtcctggcgc gctgatcggc accgcggaga tgcacggcat
      541 ccagttcgcc gtggatcagg cgaacgccaa aggcggggtg cacggcaata agatcgaagt
      601 gcagttcgag gacaatcagg ccaagcctga tcagtccgtg ctgagcttca acaagctgac
      661 cgacctggag cacgtctcgg tcatcttcac cggctattcg gggccgacac tcgccatggc
      721 gcctctggca acgcgaaaga aggtgctgct ggtgaatgct ggtgcgcagg ccgatgcatt
      781 ggcgaaggcg tcgccgtacc tgatcaatac gctgccgacg atcggtgacg agatcgcggt
      841 gttgtcgaag tggctggtgg gcgagggaaa gaagcaaggc gccatcatgt tcgagaatga
      901 tgcggccggc atgtccgggc gcgatgacta cctgcgttcg ttccccgatg ccggcggcaa
      961 ggtgctcgcg caggaagcca cacaattcgg ccagaccgat ttccgtcccg cgctgctgaa
     1021 gctggccgat gcgaagcccg atgtgatgct ggtggcgatc accgcgggtc tgctgcagat
     1081 ggcgcaggaa taccaccagc ttgggctgaa atttactgtg gcggggacaa cgttctttgc
     1141 cgatccggcg acgattgccg atccgtcatc ggcgggattt gtgcataccc aactgcggat
     1201 tgacgcgccg ccagaactcg ccgcccagtt caaggcgaaa tacggcgtgg acatggaatt
     1261 cttcgccaaa cagtattaca gcgccgcaca gatcgtgctg accaccctcg acaaggtgct
     1321 gtccgagaag cagcctgtca ccggcgagaa catgcacgac acgttgctga agatcggcaa
     1381 gttccagggc ctgatcccgc tggagttcaa gaccaacacc gcgagcgtgc cgatcgacat
     1441 caatgtgatg cgcgacggca aggacgtggc gatcaagcac atacagtagc gcacgttcca
     1501 tgctcgcgct gcaactgctg gccactggcg tggttaccgg ctgcgccctg ggtgtggtcg
     1561 cgatcagctt cgcgctggtc tatgcgacca cgaagatctt ccacgtcgcg cacgcgggta
     1621 tctacacgtt ggctggatat ctggcgtggt ctttggtgct gtggggtgcg ccgttgatcg
     1681 tcgcggtcgt ggtatcgatc gccatttgca ccgcattcgg cgcgctgatc caaagccagc
     1741 tttatgcccg cctggagcga cggcgggcca cccatctggt ggtgctgatc gcctcgctcg
     1801 gggcgcttgc ggtgatccag aacgtcatcg ccggcgtcta tacgccgaac atcctgcaat
     1861 tcccgctggc atggagcggt gaggtattgc cgctcggttc ggtgcggctg acgtatgtgc
     1921 agctgctgac gatcgctgtc agtctcgcgg cttatgttgg tacgatgtgg tttgcccatc
     1981 acaccatcct gggcaagcgg atccgggcgg tcgcctcgaa tccgatgctg gcggacatca
     2041 cgcggcttca gccccagtcc gtgtatatct atgtcatcgc cattgcctcg gccctggtgt
     2101 gcctgcctgg catactggtg ccgatggacc tcggcctgca gccctacaac ggggttacgc
     2161 ccttgctgac cgcgacgatc gcgatgatcg ccggcggcgt cggcagcatc accggcgcgt
     2221 tcgtgctctc cgtggcgatc gccgtgctcc agaacctgtc gctgctggtg atgccgggtg
     2281 agtggagcat cggcgtcacc ttcttcatct tcgtgatctt catgctgttc cggcccaccg
     2341 gccttttcgc tgcggcacgc tgatggactt tctctacagc tacatcgtgc tggccgaaat
     2401 ctacgtcctg ctgggcctgt ccaccaacct gctggttgga attgccggca tcttctcggt
     2461 gtcgcaggcg gcagtgttcg gtgttggcgc ctatctggtc gcacatttcc tgatggcgca
     2521 ggacgcgctg ccattcgtcc cctcactcgc catcgccgcg gcgtgctgcg tggctctgaa
     2581 catcctcgtc acgctgccat cgctgcgtgt cgccggcgat tatttcgtgg tgacctcgtt
     2641 cggaatccaa ttgctggcga cagccgtttt caccaactgg accgcgggca caggtggcgc
     2701 caacggcctg cccggcattc cgccccctga catcctcggc ctgcagatcg aaagcaccca
     2761 gcaatatgtg gtgctatgca ccgtcgcggc cgggcttggc tgcctgtgct tcttcctggt
     2821 gatgcgcgca cccttcggcc gcttgctgcg cgcaatccgt gaggatgagc tcgctgtcgc
     2881 tgccgccggc aagaacgtgc tgcgcgcgaa agtctccgcc gcggcgctgg cgggcgcatt
     2941 cgcgggcatc gcgggcgggt tgtacgcgac gttcctaagc ttcatcgatc cgtcatcctt
     3001 cgacctcgac gcatcgatcc tgctgctgac gatggtggtt gtgggcggcg cacgcacgct
     3061 cgccggctcg attatcggac cgttcctgct gctggccctg ccacaggtgc tgacgctgat
     3121 cgatatcccc accacgatcg ccgcctccgc gcgccagttg atctatggcg tgctgctgat
     3181 cgccttcatg ctgttccgcc cgcaaggcct ggcgggggaa cgcctgtgac cgcgtatcta
     3241 caggccgaga acctcgacca tcacttcggc ggcctgcgcg cgctacgcga atgcacgttc
     3301 gccatcaatc aaggccgcat cacctgcctg gttggtccca atggcgccgg caagaccacc
     3361 atcttcaacg ccatcaccgg cttcctgaag ccggacgaag gcagcgtcac gttcaagggc
     3421 cgcaagctgg acggcctgcg tccgcaggcc atcgtgcatg ccggcatcgc gcgcaccttc
     3481 cagaacctgc gcctgttcac cgacctgacc gcgctggaca acgtgatggt cggcatcggc
     3541 ggccagtttg gcgaagaacc cgtcggcgcg atttttcgcc cgctgcaca
//
LOCUS       DATGEM010000095         4441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_85765, whole genome shotgun sequence.
ACCESSION   DATGEM010000095 DATGEM010000000
VERSION     DATGEM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85765"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VKQ27_03035"
     CDS             complement(<1..1002)
                     /locus_tag="VKQ27_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87936.1"
                     /translation="MPSSDRAMSKGERGYLLLLAAAALYPAVILIRWVVGAIPFRYGL
                     DYNEGIVWQQMNDIVAGRGYAPINGFPAIVYHYPPVYHLLSALVAAPGIDPLSAGRIV
                     SLITTIGSALVIASFARMWLADDFSRMQRIAGIAGAVVCYLGCKGVQDWAPLMRVDPT
                     ACFFALLGLWLLVRAVQSPRLIYAAAVFFVLSVFSKQNSVLTAAAGFGVLAFWRPGLA
                     LRGVVTCILLGSAALAVLIWQTDGEVLRHLILYNINRFDVMRFLPNFWFGTSRGDRIL
                     LVLGMGGLVWTLLRWRALRGQEGIPADRMMARQATLAFALIATLSLISTAKYGSSSAY
                     "
     gene            complement(1004..1648)
                     /locus_tag="VKQ27_03040"
     CDS             complement(1004..1648)
                     /locus_tag="VKQ27_03040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY87937.1"
                     /translation="MNEGYHDTRLAHDPKRARLWRALWRYFFSKRVRSEDVVLDLGAG
                     YGDFINSVTARRRIAVDIWPGVAEHAAPGVDVVIGTADQLDSLADGSIDYAFASNLFE
                     HLTQEQFAGALRALRRVMSPRGRLTILQPNYRYAYREYFDDYTHVAVYSHVSLADFLE
                     AQGWAVEENRPRFLPLTVKSRLPAWGFLIGAYLASPFKPMGKQMLLVARPRAQG"
     gene            complement(1645..2631)
                     /locus_tag="VKQ27_03045"
     CDS             complement(1645..2631)
                     /locus_tag="VKQ27_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY87938.1"
                     /translation="MLPRRSHPPYLLYGLLSVSLGLAVYMSGVALFLPRYLLPFHHLL
                     DQVAEWLVWYSGIPLVLGFGLIAVDLLYLLQHRKPDRPVRNDVLTTDRVTVALTAYND
                     EESIADAVADFLAHPAVARVIVVSNNSSDRTLERAIAAGAIGVNEEQPGYGRCVYRCL
                     TEAMRYTDTELVVLCEGDRTFRAYDINKLLAYAAHCDIVNGTRITEPLRQYETQLSTF
                     MYYGNLYVAKLLEAKHLGRGTLTDVGTTYKLCRRSALERLLPEVDPAINLEFNAHFID
                     TALSLDMVVVECPLTFHQRVGVSKGGNINNWRGLTVGLRMIRGIVFDWRWAA"
     gene            2768..4099
                     /locus_tag="VKQ27_03050"
     CDS             2768..4099
                     /locus_tag="VKQ27_03050"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HLY87939.1"
                     /translation="MATVLGLTLPDRATAPFCPSEVEGSILVPAGTPLATRIARFAGP
                     GLLIAVGYMDPGNWATDIAAGSGYGFDLLFVVAGASLAAILLQSLALRLGMASGLDLA
                     QACRRRYGRIPNLTLWALAEIAILATDVAEVLGGALAVKLLLGLPLWAGIILTAFDML
                     IVVGLKGRGFRQVEAIVAGLVGTIALCFLIELLIVPPDMAALAAGLVPDLGRLSQPGA
                     LAVAIGVIGATVMPHNLYLHSSIVQTREVPDSLVEKRRAIGIANWDSAISLLLAALIN
                     GAILTLAAGAFHTTGHNNVTGIDEAYRLLSPITGAKAAALFFGIALFASGQSSTFTGT
                     IAGQVILEGFLHLKIPCWQRRIITRTIAIVPAMIGVVLWGDHGVGPMLVLSQIILSLQ
                     LPFAIWPLIRAAGDRSIMGELVAGPLPSMIAWGLFAIITAANLWLIASLFA"
     gene            4129..>4441
                     /locus_tag="VKQ27_03055"
     CDS             4129..>4441
                     /locus_tag="VKQ27_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY87940.1"
                     /translation="MDARMNVTDLRVALFSGNYNYVRDGANQALNRLVEYLLRQGAAV
                     RVYSPKVKKPAFQHVGDLVGVPALPLPGRAEYRAPILLTPRVKKDLHAFRPNMVHVAS
                     PE"
BASE COUNT          834 a         1493 c         1374 g          740 t
ORIGIN      
        1 ataagcgctg ctcgatccat atttggccgt cgagatgagg gacagcgtgg cgataagcgc
       61 gaaggccagg gtcgcctgcc gggccatcat gcggtcggcg gggatgccct cctgccctcg
      121 aagcgcgcgc cagcgcagca gcgtccacac gaggccgccc atgcccagca ccagcagaat
      181 gcggtcgccg cggctggttc cgaaccagaa attgggcaag aagcgcatca catcgaagcg
      241 gttgatgttg tagaggatca ggtgccgcag cacctctcca tcggtctgcc agatcagcac
      301 cgccaacgcg gccgagccca gcaatatgca tgtcaccacg ccgcgaaggg cgagccccgg
      361 ccgccagaag gccagcacgc cgaaccccgc cgccgccgtc agcacgctgt tctgcttcga
      421 gaagacggag agaacaaaaa acacagcggc ggcgtagatc agcctgggcg actggacggc
      481 gcgaaccagc aaccagaggc cgagcagcgc aaagaagcag gcggtcggat cgacccgcat
      541 cagcggcgcc cagtcctgca cgcccttgca gccgagatag cagactacgg cccccgcaat
      601 gcccgcgatg cgctgcattc gcgaaaaatc gtcggccagc cacatgcggg cgaaggatgc
      661 gatgaccagc gccgaaccga tcgtggtaat gagcgaaacg atccgtcccg ccgacagcgg
      721 atcgatgccg ggcgcggcaa cgagcgccga cagcagatga tagaccggcg ggtaatgata
      781 aacgatcgcc gggaaaccgt tgatcggcgc atagccacgc cccgcgacga tgtcgttcat
      841 ctgctgccag acgatgccct cgttgtaatc gaggccatag cggaagggta tcgcccccac
      901 gacccagcgg atcaggatca ccgcgggata gagggcggcg gcagcgagca gcaggagata
      961 tccccgctcc cccttcgaca tcgccctgtc gctggacggc atatcagccc tgcgctctcg
     1021 gacgggccac caacagcatc tgcttgccca tgggcttgaa cgggctggcc agataggcgc
     1081 cgatcaggaa accccaggca ggcaggcggg acttgacggt cagcggcagg aacctcggac
     1141 ggttctcctc gacggcccat ccctgggcct ccagaaaatc cgcaaggctg acgtgggaat
     1201 agacggcgac gtgcgtataa tcgtcgaaat attcgcgata cgcgtagcga tagttgggct
     1261 ggaggatcgt cagccttccc cttggggaca tcactctgcg aagcgcacgg agcgcgcctg
     1321 caaactgctc ctgcgtcaaa tgttcgaaca gattggacgc gaaagcgtag tcgattgaac
     1381 cgtcggcgag cgagtccaac tgatccgccg ttccgatcac cacgtccacg ccgggcgctg
     1441 catgctccgc gacgcccggc catatgtcca ccgcgatccg gcggcgcgcc gtgacgctgt
     1501 tgatgaagtc accatagccg gcgccgagat cgagaacgac gtcttccgaa cgcacccgct
     1561 tgctgaagaa atagcgccac agcgcccgcc aaagccttgc gcgcttcgga tcatgcgcca
     1621 gccgtgtgtc gtgatacccc tcgttcatgc cgcccaccgc caatcgaaca cgataccgcg
     1681 gatcatccga agaccgaccg tcaatccgcg ccaattgttg atgttaccgc ccttgctgac
     1741 cccaacacgc tgatggaacg tcaggggaca ctcgacgaca accatatcga gcgacagagc
     1801 tgtgtcgatg aaatgggcgt tgaattcgag gttgatcgcc ggatcgacct ccggcaacaa
     1861 ccgctccagc gccgaacggc ggcacaattt atatgtcgtt cccacatcgg tcagcgtgcc
     1921 gcggccgaga tgcttggctt ccagcaactt cgccacatag aggttgccgt aatacatgaa
     1981 cgtgctgagt tgcgtttcat attggcgaag cggctcggta atacgcgttc cgttgacgat
     2041 gtcgcagtgc gccgcatagg cgaggagctt gttgatatca taggcccgga acgtccggtc
     2101 cccttcgcaa agcacgacaa gctcggtgtc tgtgtagcgc atcgcctcgg tgaggcagcg
     2161 atagacgcag cgcccatagc cgggctgttc ctcattcacg ccgatggcgc ccgccgcgat
     2221 ggcccgctcc aatgtccgat cgctgctgtt gttgctgacc acgatgacgc gcgcgaccgc
     2281 cgggtgcgcc aggaagtcgg cgaccgcatc ggcgatgctt tcctcgtcat tataggccgt
     2341 caacgcaacg gtcacgcgat ccgtcgtgag gacatcgttg cgaacgggcc tgtcaggctt
     2401 gcgatgctgc agcagataca gcaggtccac cgcgatgagc ccgaagccca agactaatgg
     2461 aataccacta taccagacca accattcagc cacctgatca agaagatggt ggaacggaag
     2521 caaatagcga ggaagaaaca gagcaacgcc ggacatatac acggccagcc ccagagaaac
     2581 ggacagcagg ccatatagca gatatggcgg gtgggaacgg cgaggaagca tggagtctca
     2641 gctcggtgtg atcgatctgg actacaggcc cagtccgcag ccgcggatac gataagggtg
     2701 ttcacctgtc cactcgcgcg accggcgccc cctatccctc gccgcgccgc gtcgccataa
     2761 gccgggcatg gcaaccgtcc tcggcctcac cctccccgat cgcgccaccg ctcccttctg
     2821 cccgtccgag gtggagggca gcatcctcgt tccggccggc acgccacttg ccacccgtat
     2881 cgcccgcttc gccggccccg gcctgctcat cgccgtcggc tatatggacc cgggcaactg
     2941 ggcgaccgac atcgccgccg gatcaggcta tggcttcgac ctgctgttcg tggtggcagg
     3001 cgccagcctc gccgcgatcc tgctgcagtc gctggccctc cggctcggca tggcgtcggg
     3061 gctcgatctc gcgcaggcct gccgccgccg ctacggccgc atccccaacc tgacgctttg
     3121 ggcgctggcc gagatcgcca tcctcgccac cgacgtcgcc gaggtgctcg gcggcgcgct
     3181 cgcggtcaag ctgctgctcg ggctgccgct ctgggccgga atcatactca ccgcgttcga
     3241 catgctgatc gtggtcggcc tcaaggggcg cggtttccgg caggtggagg cgatcgtggc
     3301 ggggctggtc ggcaccatcg cgctgtgctt cctaattgag ctcctgatcg tgccgcccga
     3361 catggcggcg ctggccgcag gactggtacc cgatctcggc cggctgtcgc agccgggcgc
     3421 gctggcggtt gcgatcgggg tgatcggcgc gacggtgatg ccgcacaatc tctacctcca
     3481 ttccagcatc gtgcagaccc gcgaggtgcc cgacagcctc gtcgagaagc gccgcgcgat
     3541 cggcatcgcc aactgggatt cggcgatctc gctgctgctc gccgcgctga tcaacggcgc
     3601 gatcctgacg ctggctgccg gcgccttcca caccaccggc cacaacaatg tgaccgggat
     3661 cgacgaggcc tatcgcctgc tctcgccgat caccggcgcc aaggcggcag cgctgttctt
     3721 cggcatcgcg ctgttcgcgt cgggccagag cagcaccttc accggcacca tcgcggggca
     3781 ggtgatcctg gagggcttcc tccacctcaa gatcccctgc tggcagcgcc gcatcatcac
     3841 ccgcaccatc gcgatcgtgc cggcgatgat cggtgtcgtg ctgtgggggg atcatggcgt
     3901 cgggccgatg ctggtgctga gccaaatcat cctgtcgctc cagctgccct tcgcgatctg
     3961 gccgttgatc cgcgcggcgg gcgatcgctc gatcatgggc gagcttgtcg caggcccgct
     4021 gcccagcatg atcgcatggg ggctgttcgc tatcatcacc gccgccaacc tctggctgat
     4081 cgcgtcgctt ttcgcctgac cccattggcc tttgcggctg agcgccctat ggacgcgcgc
     4141 atgaacgtga ccgaccttcg cgtggcgctg ttttccggca attacaatta tgtccgggac
     4201 ggcgcgaacc aggccctcaa ccggctcgtg gaatatctgc tgcggcaggg tgcggccgtg
     4261 cgcgtctatt cgcccaaggt gaagaagccg gcgttccagc atgtcggcga tctggtcggc
     4321 gtaccggcgc tcccgctgcc ggggcgcgcc gaatatcgcg caccgatcct gctcaccccg
     4381 cgggtgaaga aggacctcca cgccttccgt cccaacatgg tccatgtcgc gagccccgag
     4441 g
//
LOCUS       DATGEM010000096         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1590171, whole genome shotgun sequence.
ACCESSION   DATGEM010000096 DATGEM010000000
VERSION     DATGEM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590171"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VKQ27_03060"
     CDS             <1..353
                     /locus_tag="VKQ27_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY87941.1"
                     /translation="GPAPHVPAEDINLCTIRPDLVHAERRVALEMDPGDVLIFHSLLH
                     HYTAANRSTQRRRALQFHYHQVGLDWTTLEAHRALYHDADGEYAGCTVAKGAPFAEAS
                     SYVPSRLRSVVLMA"
     gene            374..456
                     /locus_tag="VKQ27_03065"
     tRNA            374..456
                     /locus_tag="VKQ27_03065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Leu,seq:caa)
     gene            532..1011
                     /locus_tag="VKQ27_03070"
     CDS             532..1011
                     /locus_tag="VKQ27_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HLY87942.1"
                     /translation="MGADLPFWKTKTLEQMSRDEWESLCDGCGRCCLHKLRDDDTGAL
                     SFTNVACRLLDLNSCLCGDYQHRRRRVPDCVSLTPAEVRAIDWLPPSCAYRRLAEGKD
                     LAWWHPLVSGDPDTVHTAGVSVRGRAISERKAGSFEQHIVNWPGRMPRARHLPREKE"
     gene            1011..1934
                     /locus_tag="VKQ27_03075"
     CDS             1011..1934
                     /locus_tag="VKQ27_03075"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLY87943.1"
                     /translation="MMKDALFAGVNAAVLTPMRADMSVDLDRMAAHSRWLLANGCNNL
                     GVLGTTGEANSIGISERMEILPGLIERGVPAAKMMPGTGVPALTDTVLLTRQATELGC
                     RGALLLPPFYYKNPTEDGLFAFFSEVINRVGTDIKYYLYNFPQQSAVPLSVDLIGRLL
                     KAFPGVLKGVKDSSGSYDNGRAYVENFAKDGFEVYAGDDTLLRPLLQLGSAGCITAAA
                     NVNSPIAAEVYANWNNDTGAQAHETLSATRRAIVAAPAAIPALKALMARHTGNANWHN
                     MRPPHLKLTGEQQAKLFAAFDACGVQLAKAA"
     gene            2025..>2553
                     /locus_tag="VKQ27_03080"
     CDS             2025..>2553
                     /locus_tag="VKQ27_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HLY87944.1"
                     /translation="MLALPGGPTRVEWRRNRRARRVSLRIDPCGGGVVVTLPTRAGRG
                     AGVALLMTHADWVANRLAALPQAVPFADGAIVSISGVPHRIRHVQDECGGAWLQDGEL
                     HVTGAPEFLRRRVQDFLRAEALRRLSVLVGAKSAALDVTARRITVKDTRTRWGSCASD
                     GSLAFSWRLVMTPEFV"
BASE COUNT          438 a          756 c          872 g          487 t
ORIGIN      
        1 caggtcccgc gccacatgtg cccgcggagg acatcaacct gtgcaccatc cggcctgacc
       61 ttgtgcatgc ggagcgacgg gtcgcgctgg agatggaccc gggtgacgtg ctgatcttcc
      121 acagcctgct tcaccactac acggccgcga accgctccac ccaacggcgg cgggcgctgc
      181 agttccacta tcatcaggtc gggctggact ggaccacgct ggaggcgcac cgcgcgctgt
      241 atcatgatgc ggacggtgaa tatgccggtt gcacggtggc aaagggagcg ccgtttgccg
      301 aggcctcgag ttatgttccg agccggttgc ggtcggtcgt gctgatggcc taggttgcgg
      361 ccggttccaa tacgcgcgag tggcgaaatg gtaaccgcag gggtctcaaa aacccccgga
      421 ggaaactcct tgccggttcg agtccggcct cgcgcatagt ttccggcgga tgtgatccga
      481 taagcggccc accctggcca ccggcggttc atatcggtat tctcgggcgc aatgggcgca
      541 gacctgccat tctggaaaac caagacgctg gaacagatgt cccgcgacga gtgggaatcg
      601 ctctgtgacg gctgcggccg gtgctgcctg cacaagctgc gcgacgatga caccggcgca
      661 ctgtcgttca ccaacgttgc gtgccggctg ctcgacctga acagttgcct gtgcggtgac
      721 tatcagcacc gccgccgccg cgtgccggac tgcgtctcat tgaccccggc cgaggtaagg
      781 gcgatcgact ggctaccgcc gtcgtgcgcc tatcgccggc ttgccgaagg caaggacctg
      841 gcctggtggc acccgttggt ttcgggcgat cctgatactg tgcacacggc cggcgtttcg
      901 gtgcgcggcc gtgcgatcag cgagcgcaag gcggggtcgt tcgagcagca cattgttaac
      961 tggccggggc gtatgccgcg cgccagacat ctaccgcggg agaaagagtg atgatgaagg
     1021 atgccctgtt cgcgggcgtg aatgccgctg tgctgacgcc gatgcgtgcc gatatgtcgg
     1081 tggatctgga ccgcatggca gcgcattcgc gttggctgct tgccaacggc tgcaacaatc
     1141 ttggtgtgct cggcacgacc ggcgaggcga acagcatcgg catcagtgag cggatggaga
     1201 tccttccggg gctgatcgag cgcggcgttc cggcggcgaa gatgatgccg ggaaccggcg
     1261 tgccggccct gaccgacacg gtactgctga ccaggcaggc gacggaactt ggttgccgcg
     1321 gtgcgctgct gctgccgccg ttctattaca agaaccccac tgaagacggt ctgttcgcct
     1381 tcttctccga ggtgatcaat cgcgtcggca cggacatcaa gtactacctg tacaattttc
     1441 cgcagcagtc cgcggtgccg ctgagcgttg atctgatcgg gcggttgctg aaggcgtttc
     1501 caggcgtgtt gaaaggcgtc aaggacagca gcggcagtta tgacaacggc cgcgcctatg
     1561 tggagaactt cgcgaaggac ggcttcgagg tatatgcggg tgacgatacg ctgctgcgcc
     1621 cgctgctgca gcttggcagc gcgggctgca tcactgcagc cgccaatgtg aattcgccga
     1681 tcgccgcgga ggtctatgcc aactggaaca atgatacggg tgcacaggcg catgagacgc
     1741 tcagtgcgac gcgtcgcgcc attgtggccg cgccagcagc gatccctgcg ttgaaggcgc
     1801 tgatggcgcg ccataccggt aacgccaact ggcacaacat gcgtccgccg catctgaagc
     1861 tgacaggcga acagcaggcg aagttgtttg cggcgttcga tgcatgcggc gtgcagctcg
     1921 ccaaggccgc ataaccaact tgtaagcaaa agcgcgcagc gagccggttg acgaatcggc
     1981 gcgttctgtc gtttctgagc agaatggaat tgccgcgatc ggagatgctc gcgctcccgg
     2041 gaggtccaac gcgagtcgaa tggcgacgta atcgccgcgc ccgtcgcgta agcctgcgca
     2101 ttgatccatg tggtggcggc gtggttgtca cgttgcctac gcgtgctggt cgtggcgcgg
     2161 gtgttgcatt gttgatgacg cacgccgatt gggtcgccaa ccggctcgct gcattgccgc
     2221 aggctgtgcc attcgccgat ggcgcgatcg tatctatcag tggcgtgccg catcgcatcc
     2281 gccacgtgca ggacgaatgt ggtggtgcgt ggctgcagga tggcgaattg catgtgacgg
     2341 gagcgccgga gtttttgcgt cgccgggtgc aggacttcct gcgcgccgag gcgttgcggc
     2401 ggctgtcggt actggtgggc gccaagagcg ccgcactgga cgtcactgca cgccgcatca
     2461 cggtgaagga tacgcgcacc cgttggggga gttgcgcgtc cgatggctcg ctggccttca
     2521 gttggcgtct ggtgatgacg cctgagttcg tgc
//
LOCUS       DATGEM010000097        14926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1683692, whole genome shotgun sequence.
ACCESSION   DATGEM010000097 DATGEM010000000
VERSION     DATGEM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683692"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="glyA"
                     /locus_tag="VKQ27_03085"
     CDS             complement(<1..297)
                     /gene="glyA"
                     /locus_tag="VKQ27_03085"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423351.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLY87945.1"
                     /translation="MNEQRPAASLQRFFGAPLAETDPELAEVLSAELIREQDGIELIA
                     SENVVSAAVLEAQGSVLTNKYAEGYPGRRYYGGCVHVDVAENLAIERAKRLFACH"
     gene            409..762
                     /locus_tag="VKQ27_03090"
     CDS             409..762
                     /locus_tag="VKQ27_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLY87946.1"
                     /translation="MANAYRRGIDAEAAARRALERDGFTVLAQRLRTGAGEIDIVAAK
                     PDLLSIVEVKARRTLADAAFALGPRQQARLLAAADIALAEHPEWIRDGIRFDVIVVDA
                     FGRTRRITDAFRLEN"
     gene            complement(766..2295)
                     /locus_tag="VKQ27_03095"
     CDS             complement(766..2295)
                     /locus_tag="VKQ27_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HLY87947.1"
                     /translation="MSARSRQATSLARVRTFAFSGIEAVPVEVQVQIAPGSPALLLVG
                     LPDKAVGEARERMRAALSAMGLALPPRRILINLAPADILKEGSHFDLPIALGVLAAMD
                     VMPREDLFGYAALGELSLDGTVTPVAGVLPAAIGASTLDLGLICPASQGGEAAWAGRI
                     EVLAATDLLSLINHFRGTQVLTPPEPSGIADAARIPDLADVKGMETAKRAVEIAAAGN
                     HNLLMIGPPGSGKSMLAARLPGLLPDLSPAEALEVSMIHSVAGMLDGGRLLMRPPFRE
                     PHHSASQAALTGGGQRARPGEISLAHRGVLFLDELPEFPKQTLESLRQPMETGRTTVA
                     RAAAHITYPARFQLVAAMNPCRCGYLGDASRECGRAPRCAEDYQNRISGPMLDRIDLT
                     VEVQPATAADLARAPPGEPSAAVALRVARARAAQRARYGENGPASNAEADSRDVDLVP
                     QANALAEQAMEKLHLSPRGYTRVLRVGRTIADLAGARQVERQHIAEALAFRHRMPGRK
                     W"
     gene            2375..2911
                     /locus_tag="VKQ27_03100"
     CDS             2375..2911
                     /locus_tag="VKQ27_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLY87948.1"
                     /translation="MYRIAVLVVLLAGGPACAQAEPRRIAIAPPSSEVEFRAYKLGLV
                     PLDGSFRAFSGWLSYDPENRGRCEVHLTIDAASLTVPASAMRNVVAGPDFLDVARYPS
                     LTYSGTCSGDRVDGALNMHGVTGAFPLGLTWAADGVSAEGRLVRADWGMTALPLLAGR
                     TIRIRVVIPLKMNAVAGN"
     gene            2950..4551
                     /locus_tag="VKQ27_03105"
     CDS             2950..4551
                     /locus_tag="VKQ27_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY87949.1"
                     /translation="MRLPAALVPLRNPTFRTLWLANVVAWLGTWLQNTGAGWLMTSLA
                     PRPFIVAMVQAATIMPVFLLALPGGALADIVDRRVFLIGSQIWTILAASALALLTVGH
                     MTTATLLLALTFAIGIGSALTAPAWSAIVPELVPREDLVQAIALNGIGYNLTRAVGPA
                     LAGFLILLGGSSLAFSLYAVSIISVIAALFTWHRGRRFTGLPREHLLSAMRAGVRFVR
                     NTPAVQFAMVRTIAYSIPASAPWALLPLLVRERLGLGPGMYGVILGMMGIGGVTSGML
                     LPLVRGRLDRGTTVVVCTLFSCTGILLLGLSRHWLPAALAMLLFGIGWTSAYATIQAA
                     AQLVCPPWVRARALSIYQLAQNGALTAGSFAWGWLGGWVGLSDTLLVAGVVGIGLIPL
                     VRRCSIDLTIERAPLTPAEASPVPEAPAAELVPLLRRARGRVMETVHYRVKHDQRPDF
                     LHAMADVRKVRGRAGAMFWQLYEDVAHPEGWMEVWSMESWTDHLREVTRLSDDDRAVL
                     ARVGIFRCGGDGPRPARYIAVDPQH"
     gene            complement(4553..4873)
                     /locus_tag="VKQ27_03110"
     CDS             complement(4553..4873)
                     /locus_tag="VKQ27_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87950.1"
                     /translation="MTKQLLFGLLASVALPCAALAQNPQPAPPQVTHTPVAPPSSSQP
                     PAEQIVPRHGSLSDQLSDQKGTLHPPAVDPGINAPLPKQSQGSMPVIPPPGTPGGNQA
                     VVPK"
     gene            complement(4935..5711)
                     /gene="rfbF"
                     /locus_tag="VKQ27_03115"
     CDS             complement(4935..5711)
                     /gene="rfbF"
                     /locus_tag="VKQ27_03115"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HLY87951.1"
                     /translation="MKAVILAGGMGTRISEESHLRPKPMIEIGGRPILWHVMNIYTAY
                     GVTDFVICLGYRGYMIKEYFANHVLHESDMTVDARTREITYHTNYAEPWRVTLVDTGL
                     GTMTGGRLKRIGRYLESDENFCMTYGDGVANIDIAALLEFHRRHGRLATITAVVPPGR
                     YGALLLDGDVVTRFSEKPPGDNALINGGFFVLHPSVLERIECDDTPWETAPLESLARD
                     RELCAYRHTGFWQPMDTMRDKATLEALWESGRPPWKVWSD"
     gene            complement(5708..6343)
                     /locus_tag="VKQ27_03120"
     CDS             complement(5708..6343)
                     /locus_tag="VKQ27_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HLY87952.1"
                     /translation="MKYVYGFTWMGRPVIQLPEDMIRIQEVIWRIRPDVIVETGVAHG
                     GSLIFYASLFEAMGHGRVIGVDIDIREHNRAAIEAHPLSHRIELIQGSSTSPCTVSAV
                     RRRVTPDESVLVVLDSDHSRAHVEAELDHYAPMVSPGSYIVACDGIMAQLAGAPRTAP
                     DWTWNNPTAAVEAFLARNPNFVLDEPGFPFNEGVVRKRVTYWPKAFVRRVS"
     gene            complement(6340..7305)
                     /locus_tag="VKQ27_03125"
     CDS             complement(6340..7305)
                     /locus_tag="VKQ27_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLY87953.1"
                     /translation="MSAEKQSFTILGAGGFIGAAVSARLTRLGCNVRAVTRASLPHLF
                     AARRDAGHVINCIGLTGDFRARPLDTAEAHVGLVARCLAELQFESFLLLSSTRVYARA
                     ISTLEDAALATVPAEPSDLYNLTKLAGEALCLADPRPTVRVARLSNVYGARMPAETFL
                     GQVLAEGMNSGSVLFRQSPWSAKDYISIATVARLLPVIGMGGRHRLYNLACGHNTPHA
                     AIADLLHAVAGWRAGFAPDAPTLINVPIDTTRITTEFGAITCDLLADLPSIIPAEQED
                     QCSPSTSHLAALPSGIRTVPSKNTVSALPRRSRPFPPHGSAVAGT"
     gene            complement(7362..7859)
                     /locus_tag="VKQ27_03130"
     CDS             complement(7362..7859)
                     /locus_tag="VKQ27_03130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLY87954.1"
                     /translation="MLKIARSARISRLADIEDSQRGTNIIVEDDVIIDAFVKLKPAGG
                     SGDVVIGRGTVINAGCVLYSGNGIRIGRNVLIAANCTLAPTNHAFADPDRPIREQGFQ
                     PSRGGIEIGDDVWIGASCVLLDGAVIGDGAVIGASSLVRGHLPSYCLAHGTPATVHGW
                     RARQR"
     gene            complement(7853..13642)
                     /locus_tag="VKQ27_03135"
     CDS             complement(7853..13642)
                     /locus_tag="VKQ27_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY87955.1"
                     /translation="MTKAARVARQNAQSAARAATPGAVVAWQRGKPEFAEAGLKAALL
                     SNPSDRTVSSALEGMAHKPGEAKTRHRQPVAAELVDPIALCNLGIALQREGQHAEAAK
                     QLRAAIALDPGCGKAWHRLGHAMRELGNYLDALHAYHKAAIHLPGSADTHGDYGAMLF
                     GLNRNQEAYEELHRALTLDPSNASAHGNLSALLLRTGYPIAAESECRAAIAREPTQYR
                     WLTNLGVALLMQCRFSEAAECARKALAQNPDYAIAHGNLLFDLTYRSDLSATSIFAEF
                     LEWDRRHARPLAPTNPNFALDRSPGRKLRVGYVSADFRQHAVALFAEPLLRAHDRTCV
                     ELYIYSGVAAEDATTQRFRALADQWCDTVALNDAQLAQRIRGDRIDVLVDLSGHSACN
                     RLLTFARRPAPVQVAYLLGHGYTTGLSQMDAFLADEALAPPGSEAFFSERIVRLPRIP
                     LAYEPPHDMPPVEPLPAPRRGCVTFGYFGRTERLNDQVISAWARILSATPRARLVLNN
                     LPFHEPAFRALFLARFARHGIASDRLDLVYTEPQPLTWAAYADIDIALDPFPHNAGTT
                     TIEALWQGVPVISLAGRPSFGRLGASILHAAGLDDWVTDHLDQYVARAIAAAADLPAL
                     ARLREQLRPRMERSPLRDASGLARSVERSFRMLWDEWRLGDSTNLRQLYQAGDTTAAT
                     DVAHRILDRDPDNAEAHHVISLVAYRDNRLTDADQHLLIAIRSTPHRSELHANHAAIL
                     RKQGRLVEAEAAARTALRLEPNSVGALNNLGNILRDTGRYDEGAQHFRAAVRLVPDFA
                     DAWVNLAWLLSLAGQAQDAEDAARRAIACAPDNADAHNNLGLALMRQGRLAEAEAALR
                     HALALRPDFALPHSNILFCLNYRPDITPEQVFAEYQRWDRQHAQSLCPKDASYELDRS
                     PTRKLRVGYVSPDFRQHAVALFAEPLLANHDRSAIEVHCYAEVAAPDAVTERFRALAD
                     HWHQSVGLSDAALAEQIRADRIDILVDMAGHTAGNRLLAFARKPAPIQLTYLLGHGYS
                     SGLSAMDGFLADVQLVPPDADHLFSEQVIRLPRIPLCYQPPAGMPDVGPLPARSNGHI
                     TFGYFGRTVRLNDKVIATWARILRCVPNARLMLNSAPFGEAAGRERMAARFAALGIDT
                     DRLTLICTSPQPATWAAYADIDIALDPFPHNAGTTTIEALWQGVPVVSLAGRPTVGRF
                     GAAILHATGLDDWVTHDVESYVARATEAAGDPDALDGLRAALRARIAASPLRDAAGLA
                     QHIESVYRTLWQNWCDGDDTARMRQRYAAGDHADATALAEAILARNPNHTGALHILGL
                     VRLNAGDASSATALLRRAAAVHADAAILSDLGVALRSQQRFSEAEAAYREALRLDPTS
                     IHALGNLGNVLLDMHRSHEAEAELRRALHLAPNRPWLLHSLALALLAQGAVQGAEIVL
                     RQALAVDPESTDAHETLAILLAQNGRPVESAAHHRAALANQTQRHRVLSNLATALQVQ
                     GRHQEAIDACRQALAERPDYATAHSNLLFALNYASGVSPEEAFAEYRAWDRRHAAPLA
                     PAKPEFALDRSCTRRLRIGYVSADFRAHAVALFAEPLLAAHDRSGTDVICYADVAAPD
                     ATTRRFHALADQWRDITGLSDVAVADMIRQDQVDVLVDLTGHTAGSRLLVFARKPAPV
                     QVTCLLGHGYSSGMSAMDVFLSDAALTPPGSERYFSERIVRLPRLPLAYAPPENMPPV
                     SELPALADGTITFGYFGRPDRLTDEVAAAWARILDAVPGSRLVLNSFSFREPAFRALV
                     ATRFIRFGVARERLRMLATAPQPRTWAAYQAIDIALDPFPHNAGTTTIEALWQGIPVI
                     SLAGRPSVGRFGAMIMHAIGMDDWVTHDVDDYIARAVAMACDLPALAQTRAELRPCVA
                     ASPLCDAADLARHVETAYREAWNAWQQSC"
     gene            complement(13652..>14926)
                     /locus_tag="VKQ27_03140"
     CDS             complement(13652..>14926)
                     /locus_tag="VKQ27_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HLY87956.1"
                     /translation="LGTAVSNASLDLGTGSDTLTLANGANTASVSNVETITGGTGNDT
                     ITLAAAVSNGSTDLGSGSDKLTFGNFTNSGSVSNVETILGGTGNDAVTLGSALTTAMS
                     IDLGTGNNVLTFANGANTGTVKDIGTLIGGTGADTITLGTAVSNGSIDLGTGNDTLVL
                     GNFTNSATVANVETITGGTGNDTITLGNALAAAMSVDLNSGNDKLTLGNAGNTGSVSN
                     VETLIGGSGTDAITFKTAAVGASMDLGSGNDTLQLANFTNRVSVTNTETVFGGTSNDT
                     IVLTGSNASLVVGGAGINFITGNTGVDQFVFDQNSAGNYSTIQNFSAAAGDKIALDTT
                     ASATLATNTYDLNGGALADGTTLKAAADAASRLAVPTMNGGHGAFVYEQDTGELYYSG
                     NGSFAGGGTLVGVIDSNSGTPWIYNANSFTQV"
BASE COUNT         2508 a         4549 c         5122 g         2747 t
ORIGIN      
        1 atggcaggcg aagagccgct tggcgcgctc tatggccagg ttctcggcca catcgacgtg
       61 gacgcagcca ccgtagtagc gccggccggg atagccctcg gcgtatttgt tggtcagcac
      121 cgagccttgc gcctcgagca ctgcggcgga gaccacattc tcgctggcga tcagctcgat
      181 gccgtcctgc tcgcggatca gttcggcgct gagtacctcg gcgagctcgg gatcggtctc
      241 ggcaagcggg gcgccaaaga accgctgcag actggcggcg ggacgttgtt cgttcatact
      301 gtgcccacac tgtggtgatc ggcggaagct gtagcatggg gtcggtggtg cgaccaacgc
      361 caccagggcc ttcattccgc attagccgac acgcgccaca atcatggcat ggcgaatgcg
      421 tatcggcgtg gcatcgatgc cgaagcggcg gcgcggcgcg cgttggaacg cgacgggttc
      481 accgtgctgg cacagcggct gcgcaccggg gcaggcgaaa tcgatatcgt cgcagccaag
      541 cccgatctgc tgtcgattgt cgaagtgaag gcacgccgga cgctggcgga tgccgcattt
      601 gcgctcgggc cgcggcagca agcccggctg ctggcggcgg ccgacatcgc gctggcggag
      661 catccggaat ggatcaggga tgggatccgg tttgacgtga tcgtcgtgga tgcattcggc
      721 cggacacgcc ggatcaccga tgcattccgg ctggagaact gatcctcacc atttgcggcc
      781 gggcatgcgg tgccggaatg ccagcgcctc ggcgatatgc tgccgctcca cctgcctggc
      841 accggccagg tcggcgatgg tgcggccgac gcgcaacacg cgggtatagc cgcggggcga
      901 gaggtgcagc ttctccatgg cctgttcggc gagcgcgttc gcctgcggaa ccaggtcgac
      961 gtcgcggctg tctgcctcgg cattgcttgc cgggccgttc tcgccatagc gcgcccgctg
     1021 ggccgcccgg gctcgtgcga cgcgaagcgc cacggcggcg ctcggctccc caggcggcgc
     1081 gcgcgccaga tcggcggccg tggcgggctg aacctccacc gtcaggtcga tccggtccag
     1141 catcgggccg ctgatgcggt tctgataatc ctcggcacag cgcggcgcgc gcccgcattc
     1201 gcgcgacgca tcccccaggt agccgcaacg gcacgggttc atcgccgcca ccaattgaaa
     1261 gcgcgccgga taggtgatgt gggcggcggc gcgtgcgacg gtggtgcgcc cggtttccat
     1321 aggctggcgc agcgactcca gcgtttgctt ggggaattcc gggagttcgt cgaggaacag
     1381 cacgccacgg tgcgccaggc tgatctcccc cggtctcgcc cgctggccgc cgccggtgag
     1441 tgcagcctgt gacgcggagt gatgcggctc gcggaacggc ggacgcatca gcaaccggcc
     1501 gccgtccagc atgccggcaa cactgtggat catgctgact tccagcgcct cggcgggtga
     1561 cagatccggc agcaggccag gcagacgcgc cgccagcatc gactttcctg aacctggcgg
     1621 gccgatcatc agcagattgt ggttgccggc ggcggcgatc tcgaccgccc gtttggcggt
     1681 ttccatgccc ttcacatccg caaggtcagg gatgcgagct gcatcggcga tgccagaggg
     1741 ttccggtggc gtcagcacct gggtgccgcg gaagtggttg atcagcgaca acaggtcggt
     1801 ggcggcgagc acttcgatcc ggccagccca ggccgcttcg ccaccctggc tggccgggca
     1861 gatcagcccg agatcgaggg tcgaggcacc gatggccgcc ggcaacacgc cggcgaccgg
     1921 ggtcaccgtg ccatccagag acagttcgcc aagtgccgcg tagccgaaca ggtcttcgcg
     1981 cggcatgacg tccatcgccg cgaggacgcc gagcgcaatc ggcagatcga agtggctgcc
     2041 ttccttcagg atgtcggcgg gggcgaggtt gatcaggatc cgccgtggcg gcagcgccag
     2101 tcccatcgca ctgagcgcgg cacgcatccg ttcccgcgcc tctccaaccg ccttgtctgg
     2161 cagcccgacc agcaggagcg ccggcgaacc gggcgcgatc tgcacctgaa cctcgaccgg
     2221 cactgcctcg ataccggaaa aagcgaaggt gcggacacgc gcaagactgg tggcctgacg
     2281 cgatctggcg gacacgatag ggggcaggtt agccgatgat ggtgaatgca aaggaaacgg
     2341 tggcgcgatc cggaggcgtc cccacctgag ccggatgtac cgcattgccg tcctggtggt
     2401 tttgctggct ggtgggcccg cctgcgctca agccgagcct cgccgcatcg cgatcgcgcc
     2461 gccctcgagc gaggtcgaat tccgcgccta caagttgggt ctggtgccgc tcgacgggag
     2521 cttccgcgcg ttcagcggct ggctgtcgta cgatccggag aaccgggggc gctgcgaggt
     2581 ccatctcacg atcgatgcgg ccagcctcac tgtacccgcg tcggcaatgc ggaacgtcgt
     2641 cgccggcccc gatttccttg acgtcgcgcg gtatccctca ctgacctata gcgggacgtg
     2701 cagcggcgac cgggtcgacg gcgcgctcaa catgcatggc gtcaccggag ctttcccctt
     2761 ggggctgaca tgggctgcgg acggggtctc tgccgagggg cggctggtgc gcgccgactg
     2821 gggcatgacg gcacttccct tgctggccgg gcgcaccatt cgcatccggg tcgttattcc
     2881 gttgaagatg aacgcggtgg cggggaacta ggggcgccaa atcctttcgg ctccggctat
     2941 aaggcgtgca tgcgactgcc cgccgctctg gttccgctcc gcaatccgac ctttcgcact
     3001 ctctggctcg ccaatgtggt ggcctggctg gggacttggc tgcaaaacac cggcgccggc
     3061 tggctgatga ccagcctcgc gccgcgaccg ttcatcgttg caatggtgca ggcggcaacc
     3121 atcatgccgg tgttcctgct ggcacttccc ggcggagcgc tggccgatat tgtcgatcgc
     3181 cgggtgttcc tcatcgggag ccagatctgg accattcttg cggcgagcgc cctggcgctg
     3241 ctcaccgtcg gacacatgac cacggcgacg ctgctgctcg cattgacctt cgccatcggc
     3301 atcggttcgg cgctgaccgc gccggcgtgg agcgccattg taccggaact cgtgccgcgc
     3361 gaggacctcg tgcaggcgat tgccctcaac ggcatcggct acaatctcac ccgggccgtc
     3421 gggcctgcgc tggccggttt cctgatcctg ctgggcggct ccagcctggc cttctcgctc
     3481 tacgctgtgt ccataatctc tgtgatcgcg gccctgttca cgtggcaccg cggacgccgc
     3541 ttcaccggtt tgccgcgcga gcaccttctg tcggcgatgc gtgccggcgt gcgcttcgtg
     3601 cgcaatacgc cggcagtgca gttcgcgatg gtgcggacga tcgcctactc gattccggcc
     3661 tcggcgccgt gggcactgct gccgctgctg gtgcgcgagc ggctgggcct ggggcccggg
     3721 atgtacgggg tgatcctcgg catgatgggt atcggcggcg tcacttccgg gatgctgctg
     3781 ccgctggtgc gcggccggct cgatcgcggc acgacggtgg ttgtgtgcac gctgttttcg
     3841 tgtaccggga tcctgctgct gggactgtcg cggcattggc tgccggccgc gcttgccatg
     3901 ctgctgttcg gcatcggctg gacatccgca tacgccacga tccaggcagc ggcgcagctc
     3961 gtttgtccac cttgggtgcg tgcgcgggcg ctgtccatct atcagctggc gcaaaatggc
     4021 gccttgaccg cgggttcatt tgcctggggt tggcttgggg gatgggtggg cttgtccgat
     4081 acgctgctgg tcgcgggcgt ggtcggcatc ggcctgatcc cgctggtgag gcgctgttcc
     4141 atcgacctga ccatcgaaag ggcgcctctg acgccggcgg aggcatcgcc cgtcccggag
     4201 gcgccggctg ccgaacttgt tccgctgctg cggcgggctc gcgggcgcgt gatggagacc
     4261 gtccactatc gcgtgaagca tgaccagcga ccggacttcc tccatgccat ggccgacgtc
     4321 cgcaaggtgc gcggacgcgc aggcgcgatg ttctggcagc tctatgagga tgtggcgcat
     4381 ccggagggct ggatggaagt gtggtcgatg gaaagctgga ccgaccacct gcgcgaggtg
     4441 acccgcctgt ctgacgacga tcgcgcggtg ttggcgcgcg tggggatatt ccgatgcggt
     4501 ggtgatgggc cacgccccgc gcgctacatc gcagtggacc cgcagcactg acctatttgg
     4561 gaaccacggc ctgattgccg cctggtgttc cgggtggtgg aatcaccggc atcgaacctt
     4621 gggactgctt gggcagtggc gcgttgatgc ctggatcgac cgcaggtgga tgcagcgtac
     4681 ctttctggtc cgacaactga tcactcaagc tgccgtgacg cgggacgatt tgctccgccg
     4741 gcggttgcga ggagcttggc ggtgcgacgg gcgtgtgggt aacctgtggc ggtgccggtt
     4801 gcgggttctg ggcgagcgcg gcgcagggca gcgcgacgga tgccaagagg ccgaacagaa
     4861 gctgcttcgt catgacccct ccggggacgt tgtccagtta gagacaacgc agggggggcg
     4921 gcgggcgttc cagctcagtc gctccatacc ttccacggcg gcctgccgga ctcccaaagc
     4981 gcctcgagcg tcgccttgtc gcgcatcgtg tccattggct gccagaagcc ggtatggcgg
     5041 taggcgcaca actcccgatc acgcgcgagg ctctcgaggg gggcagtttc ccagggcgtg
     5101 tcgtcgcatt caatgcgctc cagcacgctc ggatgcagca cgaaaaagcc gccgttgatc
     5161 aaggcgttgt ctcccggcgg cttttcgctg aatcgggtca cgacgtctcc gtccagcagc
     5221 agagcgccat agcggcccgg cggcacaacg gccgtgatcg tggcgagcct gccgtgccgc
     5281 cggtggaatt cgagcagcgc cgcgatatcg atgttggcca cgccgtcgcc gtaggtcatg
     5341 cagaagttct cgtcgctctc gagatagcgg ccgatgcgct tgagacgtcc gccggtcatg
     5401 gtgccgaggc cagtatcgac cagcgtcacg cgccagggtt cggcgtaatt cgtatgatag
     5461 gtgatctcac gggtgcgtgc atccaccgtc atgtctgatt cgtgcagcac gtgattggcg
     5521 aaatactcct tgatcatgta gccgcgatag ccgaggcaga tcacgaagtc agtcacgcca
     5581 tacgcggtgt agatgttcat gacgtgccac aggatcgggc ggccaccgat ctcgatcatc
     5641 ggcttcgggc gcagatggga ttcttccgag atgcgggtgc ccatgccgcc cgcgaggatc
     5701 accgccttca tgacacgcgc cgcacgaagg cctttggcca ataggtgacg cgcttgcgga
     5761 cgacgccttc gttgaacggg aagcctggct cgtccagcac gaagttcgga ttgcgtgcca
     5821 ggaacgcctc gaccgcggcg gtcgggttgt tccatgtcca atcgggagcg gtgcgcggcg
     5881 cgccggcgag ctgcgccatg atgccatcgc acgccacgat gtatgacccg ggcgagacca
     5941 tgggtgcgta gtggtcgagc tccgcctcga catgcgcgcg gctgtgatcg gaatcgagca
     6001 cgacgagcac gctttcatct ggcgtaacgc ggcggcgcac ggccgagacc gtgcaagggg
     6061 aggtggagct gccttggatc agctcgatac gatgcgatag cggatgcgcc tcgatggcgg
     6121 cgcggttgtg ttcgcggatg tcgatgtcca cgccgatgac acggccatga cccatcgcct
     6181 cgaacaggct ggcatagaag atcagcgagc cgccatgcgc gacaccggtt tccacgatca
     6241 cgtcggggcg gatgcgccag atcacctcct ggatgcggat catgtcctcc ggcaactgga
     6301 tgacggggcg gcccatccaa gtgaagccat agacatattt cacgtcccag ccacagcgga
     6361 gccatgcggc ggaaacggcc tcgaacgcct cgggagagcc gagaccgtgt tcttcgacgg
     6421 aaccgtccgg atgccggacg gtaacgcggc caagtgactc gtcgatggtg agcattggtc
     6481 ttcctgttca gccggaatga tcgaaggcag gtccgccaac agatcgcagg tgatcgcacc
     6541 gaattcggtg gtgattcgcg ttgtgtcgat cggcacattg atgagggtgg gggcatcggg
     6601 agcgaacccg gcgcgccagc cggcaacggc gtgcaacagg tccgcgatgg ctgcgtgcgg
     6661 ggtgttgtgt ccgcaagcaa ggttgtagag gcggtgccgg ccccccatgc caatcaccgg
     6721 caatagtcgg gcgaccgtgg cgatgctgat atagtccttg gcggaccaag ggctttggcg
     6781 aaacagcacg ctgccgctgt tcatgccctc agcgagcacc tgtccgagaa acgtttctgc
     6841 cggcattcgc gcgccgtaaa cgttcgacag gcgcgccacg cggacagtgg ggcgcgggtc
     6901 ggccagacat agcgcttcac cggcgagttt cgtcagattg tacagatcgg aaggttcggc
     6961 gggcacggtg gccagggcgg catcctcgag ggtgcttatt gcccgtgcat atacccgcgt
     7021 ggatgacagc agcaggaatg actcgaattg cagctcggca agacagcgcg cgaccaggcc
     7081 gacatgagcc tcagccgtgt cgagcggccg cgcgcggaaa tcgccggtca ggccgatgca
     7141 attgatgaca tgcccagcgt cgcgtcgcgc cgcgaacaga tgcggcaggg atgcgcgcgt
     7201 cactgcgcgg acgttgcaac ccaggcgtgt cagccttgct gagaccgccg cgccgatgaa
     7261 tccgccggcg cccaagatcg taaatgactg cttttcagcc gacatgatcg gtgcacatcc
     7321 gactgcgtca caatcaatgc ttttacactg tgtgagtaaa tttagcgttg ccttgcgcgc
     7381 cagccgtgca ctgtcgcggg tgtcccgtgg gcgaggcagt aagatggcag atgaccgcga
     7441 accagcgatg acgctccgat aaccgcaccg tcgcctatga ccgcgccgtc cagcagcacg
     7501 cagctggcac cgatccagac gtcgtcgcca atctcgatac caccgcggct cggttggaag
     7561 ccttgctcgc ggatcgggcg atctgggtcg gcaaacgcgt ggttggttgg cgcgagcgtg
     7621 cagttggcag cgatcagtac attgcgcccg atgcggatac cgttgccgct gtacagcacg
     7681 cagcccgcgt tgatgaccgt gccccgaccg atcacgacgt cgcccgagcc gccggccggc
     7741 ttcaacttga cgaaggcgtc gatgatgacg tcgtcctcga cgatgatgtt ggtgccgcgc
     7801 tgcgagtcct cgatatcggc gagccgcgag atgcgcgctg atcgggcgat tttcagcatg
     7861 actgctgcca cgcgttccaa gcttcacgat aggcagtctc gacgtgccgg gccaggtcgg
     7921 ctgcatcgca caacggcgat gcggcgacac acggccgcaa ttcggcgcgg gtctgcgcca
     7981 gggccggaag atcgcaggcc atcgctacag cgcgggcgat gtagtcatcg acgtcatggg
     8041 tgacccagtc gtccatgccg atggcatgca tgatcatagc gccgaagcga ccgacactgg
     8101 ggcgcccggc cagcgagatc accggtatgc cctgccatag tgcttcgatg gtcgtggtgc
     8161 cggcattgtg cgggaacggg tcgagggcga tgtcgatcgc ctgataggca gcccatgtgc
     8221 gcggctgcgg cgctgtcgcc agcatccgga gccgctcccg tgcaaccccg aacctgatga
     8281 accgcgtcgc cacgagcgcg cggaatgccg gctcgcggaa tgaaaagctg ttcagcacga
     8341 ggcgcgaccc tgggacagcg tcaaggatgc gtgcccatgc tgcggccacc tcgtcggtca
     8401 ggcgatccgg tcggccgaaa taaccgaacg tgatggtccc gtcggcaagc gcggggagct
     8461 cgctgaccgg tggcatgttc tcgggtggcg cgtaagcaag tggcaggcgt ggcagacgca
     8521 cgatacgctc gctgaaataa cgttcggaac cgggcggtgt gagcgcggca tcactgagga
     8581 agacgtccat cgccgacatg ccgctgctgt agccgtgacc gagcagacag gtgacctgca
     8641 cgggcgctgg tttgcgggcg aacacaagca gccggctgcc tgccgtatgg cccgtgaggt
     8701 ctaccagcac atcgacctga tcctgccgga tcatatcggc aacggcgacg tcactcaggc
     8761 cggtaatgtc tcgccactgg tccgcgagtg cgtggaaacg ccgggtggtc gcgtctggcg
     8821 ctgcgacgtc ggcgtagcag atcacgtccg taccggatcg atcatgtgcg gccaacaagg
     8881 gctccgcgaa cagcgcgacc gcgtgggctc ggaagtcggc cgacacatag ccgatacgca
     8941 gtcggcgtgt gcagcttcga tcgagcgcga attcgggctt ggcaggcgcc aatggtgcgg
     9001 cgtgccggcg gtcccacgcg cgatattccg caaaggcttc ctcgggcgaa acgccggagg
     9061 cgtagttcag cgcgaacagc aggttggaat gagccgtggc atagtccgga cgctcggcaa
     9121 gggcctggcg gcaggcatcg atggcctcct gatgccggcc ctgcacttgc agagcggtcg
     9181 cgaggttcga cagcacgcga tggcgttgcg tttgatttgc taaagcggct cggtgatgcg
     9241 ccgcgctttc aacgggtcga ccgttctgtg cgagcaggat cgccaaggtt tcgtgggcat
     9301 ccgtcgactc cggatcgacg gcgagtgcct gccgcaacac gatctcggcg ccctggacag
     9361 caccttgtgc cagcagggcg agcgccaggc tatgcagcaa ccatgggcgg ttcggcgcca
     9421 gatgcagcgc gcggcgcagt tcggcttctg cctcgtgtga ccggtgcata tcgagcagga
     9481 cattgccgag attgcccaat gcatggatcg aggtcggatc cagacggagc gcttcccgat
     9541 aggctgcctc agcttcgctg aaccgctgtt gcgaccgcaa tgccacgccg aggtccgaca
     9601 gtatcgcggc atccgcatgt acggcggccg ctcggcgcag gagggcggtg gcagagctgg
     9661 cgtcaccagc attcaaccgc accagcccaa ggatatgcag agcgccggtg tggttgggat
     9721 tgcgcgccag gatcgcctcc gcaagcgctg tggcgtctgc gtgatccccc gctgcatagc
     9781 gctggcgcat gcgtgccgta tcgtcaccgt cacaccagtt ctgccacaga gtccggtaga
     9841 cggattcgat gtgctgagcc aggccggcgg cgtcgcgcag cggcgaggcg gcgatccgtg
     9901 cccgtagcgc agcgcgcaat ccatccagcg cgtcggggtc gccggcggct tcagtggctc
     9961 gtgccacata ggattccacg tcgtgcgtga cccagtcatc aagaccagtc gcatgcagga
    10021 ttgcggcgcc gaatcgcccg acggtgggac ggcctgctaa tgacacgacc gggacgccct
    10081 gccaaagcgc ctcgatcgtc gtggtaccgg cgttgtgcgg gaagggatcg agcgcaatgt
    10141 cgatgtcggc atatgcggcc caggtggcag gctgcggact ggtgcagatc agcgtcagtc
    10201 gatctgtgtc gatgccaagt gccgcgaatc gtgccgccat ccgctcgcgt ccggcggctt
    10261 cgccgaatgg cgcgctgttc agcatgagcc gggcatttgg cacacatcgc agtatgcgag
    10321 cccaggtggc gatgactttg tcgttgaggc gaaccgtgcg gccgaaatat ccgaacgtga
    10381 tatggccatt gctgcgagcc ggcaaaggac cgacgtctgg catcccggcc ggtggttggt
    10441 agcacagcgg gatgcgcggc aggcgaatga cctgctcact gaacaggtgg tccgcatcgg
    10501 gcggcacgag ttgtacgtcg gccaggaagc catccatcgc cgacagcccc gagctgtagc
    10561 cgtggcctag gagataggtc agctgtattg gtgctggctt gcgcgcgaac gcaaggagtc
    10621 ggttgccggc agtatgtcct gccatatcga caagtatgtc gatgcggtct gcgcggatct
    10681 gctcggcgag agcggcatcg ctgagaccga ccgattgatg ccagtggtct gcgagggctc
    10741 gaaaccgctc ggtgacagca tcgggggcgg caacctcggc gtagcaatgc acctcgatcg
    10801 ctgagcggtc atggttcgcc agcagcggtt cggcgaacag tgcgacagcg tgctggcgga
    10861 aatcaggaga gacgtagccg acgcgcagct tccgtgtcgg gctgcgatcg agctcgtacg
    10921 aggcgtcttt cgggcacaat gattgcgcgt gctggcgatc ccatcgttga tattcggcga
    10981 acacctgttc aggtgttatg tcgggtcggt agttgaggca aaacagaata ttgctgtgcg
    11041 gcagcgcgaa atcgggacgc agcgcgagcg cgtgccgcag ggcggcttcg gcttcggcga
    11101 gcctgccctg gcgcatcagg gcgaggccga gattgttgtg cgcatcagca ttgtcgggag
    11161 cgcaggcaat tgctcgacgt gcagcgtcct ccgcgtcctg cgcttgtccg gccagggaca
    11221 gaagccaggc cagattcacc caggcgtcgg caaaatccgg taccaggcga accgcggcgc
    11281 ggaaatgctg ggcaccttcg tcataacggc ccgtgtcgcg cagaatgttc ccaagattgt
    11341 tgagcgcgcc cacagagttg ggttcgagcc gcaaggcggt gcgcgcggcc gcctcggctt
    11401 ccacgagacg accctgtttg cgcaggattg cggcgtggtt ggcatgcaat tctgaccggt
    11461 gcggcgtgct tctgatggcg atcagcaaat gctgatccgc gtccgtgagc cggttgtcgc
    11521 ggtatgcgac aagactgatc acatggtgcg cctcggcatt gtctgggtcg cgatcaagga
    11581 tcctgtgcgc gacgtccgtg gctgctgttg tatcccccgc ctggtagagc tgacgcagat
    11641 tggtgctgtc gcccagccgc cattcgtccc atagcattct gaatgaccgc tcgacggatc
    11701 gggcgagacc cgatgcgtcg cgcagcggcg aacgttccat gcgcggtcgc agttgctcgc
    11761 gcaatcgggc gagcgccggg aggtcggccg cggccgcgat cgcgcgcgcc acatactggt
    11821 ccaggtgatc ggtcacccag tcgtcgagac cggcggcgtg cagaatgctg gcgccaaggc
    11881 gcccgaagct gggtcgaccc gccagggata tgacaggtac gccctgccac agcgcttcga
    11941 ttgtcgtggt gcctgcgttg tgcggaaacg gatccagcgc gatgtctatg tcggcatagg
    12001 cagcccaggt gagcggctgc ggctcggtgt acaccagatc gagtcgatcc gaggcaatgc
    12061 catgccttgc gaagcgcgcc aggaacagcg cccggaatgc gggctcgtgg aacggcaggt
    12121 tattgaggac cagcctcgcc ctcggtgttg cgctcaggat ccgcgcccag gcagagatga
    12181 cctgatcatt gaggcgctcg gtccgcccga aatagccgaa cgtgacgcaa ccccgccttg
    12241 gcgcaggcag cggttcgacg ggaggcatgt catgcggggg ctcgtaggcg agcggaatcc
    12301 gtggcagccg gacgatgcgt tcgctgaaga aggcttctga ccctggcggc gcgagggctt
    12361 cgtctgccag gaacgcgtcc atttgcgaga gaccggtggt atagccgtga cccagcagat
    12421 acgcgacctg aacaggtgcc ggccttcgcg caaatgtcag cagcctgttg catgccgagt
    12481 gtcccgacag gtcgacgagc acatcaatcc tgtcgccgcg tattctttgg gcgagctgtg
    12541 catcattcag tgcgacggtg tcgcaccatt ggtcggcgag cgcgcgaaat cgctgggtgg
    12601 tagcgtcttc cgcggcgacg cccgagtaga tgtacagctc gacacaggtg cggtcgtggg
    12661 cacgaagcaa aggttcggcg aagagcgcta ccgcatgttg gcggaaatct gccgacacat
    12721 agccaacgcg cagtttacga ccagggctgc ggtccagggc gaagttcggg ttggtgggag
    12781 caaggggacg agcatgccgg cggtcccatt ccaggaattc ggcaaagatc gacgtggcgg
    12841 aaaggtcgga ccggtaggtg aggtcgaaca gaagattgcc atgcgcaatg gcgtagtccg
    12901 gattttgagc cagtgctttt cgcgcgcatt ctgccgcttc gctgaagcga cattgcatga
    12961 gcagggcaac gccgaggttg gtaagccatc gatactgcgt cggctcccgc gcgatcgcgg
    13021 ctcggcattc ggattcagcg gcgatcggat atccggtacg caacagcaat gcgctaaggt
    13081 tgccatgtgc tgaggcattc gacggatcca gtgtcagggc gcggtgaagc tcctcgtagg
    13141 cctcctgatt gcgattgagc ccaaacagca ttgcgccata gtcaccgtgc gtgtcggctg
    13201 aaccgggcaa gtggattgcg gccttgtggt atgcgtggag ggcatcgagg taattcccga
    13261 gctcacgcat ggcatgtccg aggcggtgcc acgctttgcc gcagcccgga tcgagggcga
    13321 tggccgcacg aagctgcttg gctgcctcag catgctgacc ctctctttgg agcgcgattc
    13381 caagattgca gagcgcaatg gggtccacca gttccgctgc gacaggctgt cggtgtcggg
    13441 ttttggcctc gccaggctta tgggccatac cttccagcgc gcttgagacg gtacggtcgc
    13501 ttggattgct caggagggcc gctttgagcc cggcttcagc aaactctggt ttgccgcgtt
    13561 gccacgccac gacggcacct ggggtggcag cccgggccgc gctttgtgca ttttgcctgg
    13621 cgacccgagc tgccttggtc attcactcac gctagacctg tgtgaagctg ttcgcgttgt
    13681 agatccacgg agtgccgctg ttgctgtcga tcaccccgac cagggtgccg ccaccagcaa
    13741 agcttccgtt gccgctatag tacagctcac cggtatcctg ttcgtaaacg aatgctccgt
    13801 ggccgccgtt catcgtcggg acggcaagtc gcgaggccgc atctgctgcc gccttcaaag
    13861 tggtgccgtc agccagggcg ccgccattca ggtcgtacgt gttggtagcg agtgttgcgc
    13921 tggctgtggt gtccagggcg atcttgtcgc ctgcagcagc actgaagttc tgtattgtgc
    13981 tgtagttacc tgcgctgttc tggtcgaaga caaactgatc aacgccggtg ttgccagtga
    14041 taaagttaat gccggcacca ccgacgacga ggctcgcatt tgaaccagtc agcacgatcg
    14101 tgtcgttgct ggttccgccg aagacagtct cggtgttcgt gacagaaacc cggttggtga
    14161 agttagcaag ttgcagcgtg tcgttgcccg aaccgagatc catgcttgcg cccactgctg
    14221 ccgttttgaa agtgatggcg tctgttccgg agcctccgat gagggtttca acgttgctga
    14281 cgctgccggt gttgccggca ttgcccaacg tcagcttgtc gttgccgctg ttgagatcaa
    14341 ccgacatggc ggcagcgagt gcattgccca aggtgatcgt gtcgttgcca gttccgccag
    14401 taattgtctc gacattggca accgtggccg agttcgtaaa gttgcccagg acaagcgtgt
    14461 cgttacctgt tcctagatcg atggagccat ttgatacagc ggtgcccaaa gtgatggtgt
    14521 cggcgccggt gccaccaatc agggtgccga tatctttcac tgtgccggtg ttggcgccgt
    14581 ttgcgaatgt caacacgttg ttgccggtgc caaggtcgat cgacatggcg gtggtgagcg
    14641 ccgagccgag tgtgacagcg tcattcccgg tgccacccag aatggtttca acgttgctga
    14701 cactgccgct gttggtgaag ttgccgaagg tgagcttgtc ggagcccgac ccaaggtcgg
    14761 tggacccatt ggagactgcc gccgccagag tgatggtgtc gttgccggtg ccgccggtga
    14821 tggtctcgac gttgctgacg gaggccgtgt tggcaccgtt ggcgagggtg agagtgtcgc
    14881 tgccggtgcc gagatcgagg gaggcgttgg agacggcggt gccgag
//
LOCUS       DATGEM010000098         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_315770, whole genome shotgun sequence.
ACCESSION   DATGEM010000098 DATGEM010000000
VERSION     DATGEM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315770"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VKQ27_03145"
     CDS             <1..286
                     /locus_tag="VKQ27_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY87957.1"
                     /translation="GISLRYVQKLFTERGTTCTQYLYSLRLDHAAQLLRRRAHLGKAQ
                     PLSEIAYACGYNDYSHFARNFRRRFGFPPGGLAAGQGQEPGDATREGVAA"
     gene            complement(437..1345)
                     /gene="cynR"
                     /locus_tag="VKQ27_03150"
     CDS             complement(437..1345)
                     /gene="cynR"
                     /locus_tag="VKQ27_03150"
                     /inference="COORDINATES: protein motif:HMM:NF008416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HLY87958.1"
                     /translation="MLLRHARYLVAIADHGSFTRAAAELHVSQPALSQQIRQLEEMLG
                     ARLLDRTGRIVRLTDAGRAYIGHARRALREFEAGRRAIHDVENLCSGALRLAFTPTFT
                     TYLVAPLVRQFHRLHPGVGISVNVLAQTEMEAALSEDTIDLGVAFGAVRSDDIVARPL
                     YEERLCLVVAEGHPAFAHTELAASSLDGMHLALLDTTFVTRTAIDGYLNTAAAYPRIA
                     IESNSVECLLAVLEGGGLATIMPQAAASEITGLKAIQLLPPIASRTVSVLQRDGAYST
                     AASRAFVAMLEAWDWVSLGRDPTAAL"
     gene            1492..1953
                     /locus_tag="VKQ27_03155"
     CDS             1492..1953
                     /locus_tag="VKQ27_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010517678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY87959.1"
                     /translation="MSAPVPPFTRETAIVKVRMAEDGWNSRDPQRLSLVYTEGSRWRN
                     RAEFPVGRAQILEFLTRKWAKELDYRLIKELWAFDGNRIAVRFAYEWHDDSGHWYRSY
                     GNENWEFDDVGLMQRRFASINDMPIKETDRKFHWPLGRRPDDHPGLSQLNL"
     gene            1966..>2807
                     /locus_tag="VKQ27_03160"
     CDS             1966..>2807
                     /locus_tag="VKQ27_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87960.1"
                     /translation="MLLDWRQNQHGDSMLRSDDAPGPPQLDYDAWIASLRELGARHHP
                     VANEPKSFTGWLRPFNVCGLVGLDVGCNADQIERTFQDTRLDGREHYYALIPVAGHTT
                     MSQNDQTGRLAVGDVAIVDSARAVTCLLPHAGAQWLSLQLPRRSLASHLGFEPLGGIR
                     GSDGAAAGRLLHHIVLDALGGDGLSSPRADSYMQLAVYDLLGALFAPSDPGQGSRSTD
                     KLFARVCGVMRERFPAPDFGPFDVAVQTGISLRYVQKLFTERGTTCTQYLYSLRLDHA
                     AQLLR"
BASE COUNT          512 a          872 c          923 g          500 t
ORIGIN      
        1 ggggatctcc ctgcgctacg tccagaagct gtttaccgag cgcggcacga cctgcacgca
       61 gtacctgtat tcgctgcggc tggatcacgc agcgcagctg ctgcgtcgcc gcgcgcatct
      121 tgggaaagcc cagccgctca gcgagatcgc ctatgcctgc ggctacaacg actacagcca
      181 ctttgcgcgc aatttccggc gccggttcgg gtttccgccg ggcggcctgg ccgcaggaca
      241 aggccaggag ccaggcgacg caacgcgtga gggcgtcgcc gcctgattga ctccgcgccg
      301 cccgcgccgc cggggcgtgc gcgccggtcc cggcgatcgc aggatcacct gggaatcgct
      361 gctgttgggg ggcatagctg tgagcttatg agcaacggct actcgctggc cgggtctaga
      421 agcaagctgg ccaaggttac agtgccgcag tcggatcacg ccccaaggat acccaatccc
      481 acgcctccag catcgcgacg aaggctctgc ttgccgcggt gctatatgcg ccgtcgcgct
      541 ggagaaccga aaccgttcgg ctcgcgatcg gcggcagcaa ttggatcgcc ttcaggccgg
      601 tgatctcgct cgcggccgcc tggggcatga tggttgccaa cccgccgccc tccaggacgg
      661 ccaaaaggca ctcgacggaa ttcgattcga tggcgatgcg tggatacgcg gcagcggtgt
      721 tcagatagcc atcaatcgcg gtgcgcgtga cgaaagtggt gtcgagcagc gcgaggtgca
      781 taccatcgag gctggaggcg gccagttcgg tatgggcgaa ggcgggatgt ccttcggcga
      841 cgacgagaca gaggcgctcc tcatagagtg gccgggcgac gatatcgtcc gaccgcacgg
      901 ccccgaaagc cacgccgaga tcgatcgtgt cctcggacag cgccgcctcc atttcggttt
      961 gggcaagcac attgaccgag atcccgacac ctgggtgcag ccggtggaat tgccgcacca
     1021 gcggcgcgac aagataggtt gtgaaggttg gcgtgaaagc gagccggagc gcgccgctac
     1081 agaggttctc gacgtcatga atcgcgcgcc ggccggcttc gaactcccgc agcgcgcggc
     1141 gggcgtgacc gatatacgcc cgcccagcgt cagtaagcct gacaatccgc cccgttcgat
     1201 ccagaagccg ggcgccgagc atctcctcca actgccggat ctgctgggag agcgcaggct
     1261 gggatacgtg cagctcagcc gccgcgcggg tgaagctgcc gtggtcggcg atggcgacga
     1321 ggtagcgggc gtggcgcagt agcataagca ggacttatca ggtccgtaag aaccgagtct
     1381 tagacattat gaccggcggg acgcagatat ttcccgcaga aggatcgaat ggagccaagc
     1441 catgaatgac ttcatcgctg aatttttgaa ttttcgcgag gcggcatgtc gatgagcgcg
     1501 cctgtgcctc ccttcacgcg ggaaaccgca atcgtaaaag ttcgcatggc cgaagacggc
     1561 tggaacagtc gggacccgca acgcctgtca ctggtttata cagagggcag ccggtggcgg
     1621 aaccgcgctg agttcccggt cggtcgcgcc cagatcttgg agtttctcac ccgaaaatgg
     1681 gctaaggagt tggattaccg gctgatcaag gaactgtggg cgttcgacgg caaccgcatc
     1741 gcggtgcgct tcgcctatga atggcacgac gacagcggtc attggtatcg ctcctacggg
     1801 aacgaaaatt gggaattcga tgacgtcggc ctaatgcagc gccgctttgc gtcgatcaac
     1861 gacatgccga tcaaagaaac agatcggaag ttccactggc cacttggccg gcgccctgac
     1921 gaccatcccg gcctgtccca gcttaacctc tagtggctta ggagtatgct tctggactgg
     1981 aggcaaaacc agcatggaga cagtatgttg aggagcgatg atgcaccggg tccgccgcag
     2041 ctggattatg atgcgtggat cgcttcgctg cgcgagctcg gcgcacggca ccatccggtc
     2101 gccaacgaac ccaaatcttt tacgggttgg ctgcgcccct tcaacgtctg cggtcttgtg
     2161 ggtctggacg tcggctgcaa cgctgaccag atcgaacgga cattccagga tacccggctc
     2221 gacgggcggg agcactacta cgcactgatt ccggtcgccg gccacacgac gatgagccag
     2281 aacgaccaga cggggcggct cgccgtgggc gacgtggcga tcgtcgattc agcccgcgcg
     2341 gtgacctgtc ttcttccgca cgccggcgcc caatggctgt cgctacagct gccgcgccgg
     2401 tcgctggcgt ctcatctcgg gttcgagccg ctgggtggga ttcgcgggtc cgatggagcg
     2461 gcggcggggc gactgctgca tcacatcgtt ctggacgcac tcggcggcga cggactctcg
     2521 tcgccgcggg ccgactccta catgcagctg gcggtctacg atctgctcgg cgcgctcttt
     2581 gcgccgtccg atccggggca gggctcgcgc tctaccgaca agctgttcgc acgtgtgtgt
     2641 ggcgtcatga gggagcgctt cccggccccg gatttcggac cgttcgacgt cgccgtccag
     2701 acggggatct ccctgcgcta cgtccagaag ctgtttaccg agcgcggcac gacctgcacg
     2761 cagtacctgt attcgctgcg gctggatcac gcagcgcagc tgctgcg
//
LOCUS       DATGEM010000099         8752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_215702, whole genome shotgun sequence.
ACCESSION   DATGEM010000099 DATGEM010000000
VERSION     DATGEM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215702"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..452)
                     /gene="gloA"
                     /locus_tag="VKQ27_03165"
     CDS             complement(66..452)
                     /gene="gloA"
                     /locus_tag="VKQ27_03165"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HLY87961.1"
                     /translation="MSRFLHTMLRVGNLQRSVDFYTKAFGMKELRRRDVPDGKYTLAF
                     VGYGDEDSNTAIELTYNYGVEKYEMGGAFGHLAVGVPDVAAACEIAAKAGGTITRPAG
                     PVKFGTTIIAFVKDPDGYSIELVQRP"
     gene            complement(449..1297)
                     /gene="lpxI"
                     /locus_tag="VKQ27_03170"
     CDS             complement(449..1297)
                     /gene="lpxI"
                     /locus_tag="VKQ27_03170"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559471.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HLY87962.1"
                     /translation="MKRALGILAGGGPLPGQVAAAARAAGRSVFLVGLAGFADPAVLA
                     PWPHEVVRILAAGRIIAALREHGCQDLVLVGPVRRPSLLGLRPDAEGARLLARIGRAA
                     FAGDDGLLAAVVKVFSEEGFRVVGAHEILSEALGPAGLLSRAAPDALAMADIRRGIAV
                     TRALGAVDVGQGCVVQQGIVLAVEAAEGTDAMLSRCAPLAWPGPGGVLVKLVKPGQDR
                     RADLPTIGPDTIRGARSAGLRGVAFEAGGTILAERDAAIAEADAAGLFLLGLGPDQLS
                     EMGENT"
     gene            complement(1294..2109)
                     /gene="lpxA"
                     /locus_tag="VKQ27_03175"
     CDS             complement(1294..2109)
                     /gene="lpxA"
                     /locus_tag="VKQ27_03175"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLY87963.1"
                     /translation="MPNTIALRAAGTVIHPTAIVAPGAALGIGVRIGPFCTIGANVVI
                     EDRAELVSHVVVDGHTRVGPDAILYPFCTIGLAPQDLKYAGQPTRCEIGARTQIREHC
                     TIHRGTVTGKGITRIGSDCMLMAVAHVAHDCTLGNHVIIANNAVMGGHVSIADHAVIG
                     GAAAIHQFVRIGRAAMIGGVSGVEADVIPFGSVIGNRARLAGLNVIGLKRRGFDKAQI
                     QLLHNAFRDLFRREGVFAQRLVTVRERYGNEPLIAEVLEFIDASSHRGLIRAE"
     gene            complement(2248..2721)
                     /gene="fabZ"
                     /locus_tag="VKQ27_03180"
     CDS             complement(2248..2721)
                     /gene="fabZ"
                     /locus_tag="VKQ27_03180"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HLY87964.1"
                     /translation="MVSSDTSIEVIDIARIMHAIPHRYPFLMIDRVVDVVRNKSAIGI
                     KNVSVNESFFAGHFPNHPVMPGVLIIESMAQTAAVLVVETLGPDAAGKVVYFMSIEGA
                     KFRRPVVPGDQLRIHVTKERNRGNVWKFNAVARVDGASVAEATYAAMIMDRAPPP"
     gene            complement(2751..3827)
                     /gene="lpxD"
                     /locus_tag="VKQ27_03185"
     CDS             complement(2751..3827)
                     /gene="lpxD"
                     /locus_tag="VKQ27_03185"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLY87965.1"
                     /translation="MDWSPIAGDPRFFRRSGPHSLAAVADAAQAEAPPRRLMLAGVAP
                     LQTARPDEVSFLDNRKYIEALAATQAGVVIVHPDMSANVPGTAVAVTTTEPYVAWARV
                     ASLFFPLPPLDPSVHPSAVVAPGAAIDPSAEIGALAVIGAGAEIGARTRIAPLAVIGD
                     GVVLGADCRIGSHVTISHALVGDRVVIYPGARIGQDGFGFAITSEAFLTVPQIGRVIL
                     EHDVEIGANTTIDRGSLHDTHIGAGSRLDNLVQIGHNVRLGRGCVIVSQAGISGSTIL
                     EDHVMVAGQAGLTGHLRIGRRARIGAQAGVMADVPAGADVVGSPAQPVKEFFRHVAVL
                     RRLVREAAGRRSRGTASGTETDTD"
     gene            complement(3849..4721)
                     /locus_tag="VKQ27_03190"
     CDS             complement(3849..4721)
                     /locus_tag="VKQ27_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLY87966.1"
                     /translation="MSQIRILQASVITGAVLLFQPAAAQQNQQWFVPNQPRAAAPRPA
                     QAAPPRPSIGAPAEQTAPETSLMPAEGGQPGAEGQAQVQLPPPPEVTPLPKGPTPPAA
                     IVGVLSLADVVRASSAFQQADKEFVARRQKLNEDAQKEQQTLNNLNQSLANDRAKLTP
                     EQIRTREKELQDRITESRRVFGERNRVIQEASQYVMAQIDRAIEMVTQQVAISRGINV
                     VLNRAQILGATPEFDMTPAVVEDLNKVLVKVVIPPEGVSPMSMKPPAGAAAATSENKS
                     PPAKPATPAAAKKP"
     gene            complement(4721..7174)
                     /gene="bamA"
                     /locus_tag="VKQ27_03195"
     CDS             complement(4721..7174)
                     /gene="bamA"
                     /locus_tag="VKQ27_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HLY87967.1"
                     /translation="MACCGACLLWRLAEGRRCLIRRRAAWLAAACMLVLGLVGSASAQ
                     RPAQPRPAPSVRTAAQPAAATGPVRNGGTIAAIKIDGNQRIETATVLSYMLVRPGDPF
                     DADRLDRSLKTLYATGLFQDVRLNRDGDTLVVHVVENPLVNRVAFEGNHKLTDDQLRP
                     VLQIKSRAVFTPALAEEDRQRILDIYAKHGYYNATVNAQVIRLDQNRVDVVFQINDGS
                     ITLISKIVFVGNKEFSEDRLSEVINSRESRWWRFLSTSDEYDPDRLSFDKELLRRFYL
                     KNGYIDFNILDAKSELAPDRSGFFLTFTISEGVRYRVGKIEVTSHLRNLTGDQLRGDL
                     QLDVGDWYDGDAVGRTVEVMEKDVHDRGYAFVEVKPLISKHPDKRTVDLAFDVGEGPR
                     VYVERIDIVGNTRTKDKVIRREFRLQEKDPYNGEAVRLSRQRLQDLNYFNSVNIAQSQ
                     GSTSDKTVLTTTVDEKATGELTLGGGYSTDAGALLDVGLSEKNLVGTGISAGINGVLA
                     QRRSSITASVTDPYFLDRNMVVGGDLFFVQTNFLGTEPYDEKRVGFDARVGYDFNEYL
                     RQVWSYTLVGRTVFNIASNASFFIAQEAGYSLLSQVSQSTTYDHRDSKIDPHSGYMVS
                     WGTDIAGLGGNSQFIRNNVNGQYFLPLDRFTGNSDWGLSFTGSVGYLYNEGVQEQIID
                     RFYLGGDNLRGFEIGGAGPHDAVTGDPLGGRFIWTQSTELRFPLPVSPDIGISGRAFV
                     DIGGLTGASFRNNNVCVEPSTGQACQIADSPAARLGAGVGISWRTQFGLLNVDLAPFV
                     IKQPHDQTQIFRFGFGTRF"
     gene            complement(7243..7656)
                     /locus_tag="VKQ27_03200"
     CDS             complement(7243..7656)
                     /locus_tag="VKQ27_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY87968.1"
                     /translation="MLRITQLDHLVLRVVDLRAMLHFYVDVLGCTLEKVQENIGLWQL
                     RAGNALIDLVPVNGPLGKKGGAPAGKEARNLDHFCLQVEPFDGEAIIAHLRQKGCEPG
                     PIESRYGAYGQGPSIYVPDPEGNMVELKGPPWPSA"
     gene            7801..8532
                     /locus_tag="VKQ27_03205"
     CDS             7801..8532
                     /locus_tag="VKQ27_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLY87969.1"
                     /translation="MSASTITHGHLLAGRIAYAQLRAGFRSGIEPVLLAAAVPARPGD
                     RVLEAGSGAGATLLCLMARVAGVQTTGIEKSEQLTRLARQNADANGWPAMSLIAADIA
                     EAAFTGSFDHACANPPYHPADGTASPDAARDSAKRDSPGLLAVWARALGRSLKPRGTL
                     TLILPTAALPRAVTALADSGCAPTALLPLWPKLGRPAKLALLRGVKGGRSQFRVMPGL
                     LLHTSAGDYTPEADAVLRDGAALAI"
     gene            complement(8545..>8752)
                     /locus_tag="VKQ27_03210"
     CDS             complement(8545..>8752)
                     /locus_tag="VKQ27_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87970.1"
                     /translation="DKILIAFHHACDQADFEVAEQLLHILEMMLTRRPLTPDGTRRRN
                     MESLVAAHERLWHLRHPHTGDDSF"
BASE COUNT         1624 a         2811 c         2755 g         1562 t
ORIGIN      
        1 aaccggagca cggataggtc cgtggccgag gctgatcctc ggccacggcg gcaaggacgc
       61 gatgcttacg gccgctgtac cagctcgatc gagtagccgt ccggatcctt tacgaaggcg
      121 atgatcgtgg tgccgaactt caccggtccg gcggggcggg tgatcgtgcc cccagccttg
      181 gctgcgattt cgcacgcggc ggctacgtcg ggcacgccaa cggcaaggtg accgaacgcg
      241 ccgcccatct cgtatttctc gacgccgtag ttgtaggtga gttctatcgc ggtgttgctg
      301 tcctcgtcgc cataaccgac gaaggcgagc gtatatttgc cgtccggaac atcgcggcgg
      361 cgcagttcct tcatgccgaa ggcctttgta tagaaatcga cgctgcgctg caggttgccc
      421 acacggagca tggtgtgcag aaagcggctc atgtgttctc tcccatctcg gataactggt
      481 cagggccaag gcctagcaga aatagccccg cggcgtccgc ctcggctatg gctgcatcgc
      541 gctcggcaag gatggtgcct ccagcctcga aagcaacccc gcgaaggccg gccgacctcg
      601 cgccgcggat ggtgtcgggg ccaatggtcg gcagatcggc gcggcggtcc tgtcccggct
      661 tgaccagctt caccagcacg ccgcccggcc ccggccatgc cagcggggca cagcgcgaca
      721 gcatggcatc ggtcccctcc gctgcctcca ccgccaggac gattccctgc tgcaccacgc
      781 agccctgccc cacgtccact gcacccaaag cacgggtcac cgcgatgccg cgccggatgt
      841 cggccattgc caatgcgtcg ggcgcagcac gtgagagcag cccggccggg cccagcgcct
      901 cactaaggat ctcgtgggcg ccgacgacgc gaaaaccctc ctcgctgaac accttcacca
      961 cggcggcgag caatccgtcg tcgccggcga acgcggcacg gccgatgcgc gccagcagcc
     1021 gcgcgccttc cgcatctggc cgcaacccga gcagcgacgg ccgccgcact ggcccgacca
     1081 gcacgaggtc ctggcagcca tgttcacgca gagccgcaat gatcctgccg gcagcgagaa
     1141 tgcgcacgac ctcgtgaggc cacggcgcca gaaccgccgg gtcggcaaag cccgccaatc
     1201 caaccagaaa gacactgcgg cctgccgccc gggcagcggc tgccacctgg cctggcagcg
     1261 gaccgccgcc agccagaatg ccaagggcgc gcttcactcc gcgcggatca ggccgcgatg
     1321 gctggaggcg tcgatgaatt ccaatacctc ggcgatcagg ggctcgttgc cgtagcgctc
     1381 gcgcaccgtg acgaggcgct gggcaaacac gccctcgcgg cggaacagat cgcgaaaggc
     1441 gttgtgtagc aactggatct gcgccttgtc gaagccgcgg cgcttcaggc ctatcacgtt
     1501 cagtccagcc agacgcgcgc ggttgccgat cacgctgcca aacgggatca catcggcctc
     1561 gacacccgag acgccgccta tcatcgcagc gcggccgatg cggacgaatt gatggatggc
     1621 cgcggcgccg ccgatgaccg catggtcagc gatcgataca tggccgccca tcacggcatt
     1681 gttcgcgatg atgacatggt tgccgagcgt gcaatcgtgc gcgacatgcg cgaccgccat
     1741 cagcatgcag tcagaaccga tgcgggtgat acctttgcca gtcacagtcc cacgatgaat
     1801 cgtgcagtgc tcacggatct gcgttcgcgc accgatctcg cagcgggtgg gctgaccggc
     1861 gtatttcagg tcctgcggcg cgaggccgat ggtgcagaat ggataaagga tcgcgtcggg
     1921 accgacgcgc gtgtggccgt cgaccacgac atgcgagacc agctcagcgc gatcctcgat
     1981 cacgacattc gcgccgatcg tgcagaaggg ccctatccgg acgccgatgc ccagcgccgc
     2041 accgggcgcg acaatggctg tcggatggat gacagtgccg gccgctcgca gcgcaatcgt
     2101 gttgggcatt gcatccattc tgacagcact ttcaatcgat aacagtcgtg atacggcggc
     2161 ggtgtctggc caccaacaca atcattgttg ccagatattt cgataatcgg cctgtaactg
     2221 actgtttcgc cgcacatttt ggtcatatta cggcggtggg gcacggtcca tgatcatcgc
     2281 ggcataggtc gcctcggcga cagatgcacc atcgacgcgc gccactgcgt tgaacttcca
     2341 cacgttgccg cggttgcgct ccttggtgac gtggatgcgc agctgatcgc caggcacgac
     2401 cggccggcgg aacttggcgc cctcgatcga catgaaatag accaccttgc cggcggcatc
     2461 cgggccgagc gtttccacca ccagcacggc cgccgtttgc gccatgcttt cgataatcag
     2521 cacgccgggc atcacaggat ggttgggaaa atgtccggcg aagaaggatt cgttcaccga
     2581 gacgttcttg attccaatcg cagatttgtt gcgcaccaca tcgaccacgc gatcgatcat
     2641 caggaagggg tagcgatgcg ggatcgcgtg catgatgcgc gcgatatcga tcacttcgat
     2701 ggaagtgtcg gaggaaacca tttcggtggt ggcgggcgaa tccatcacgc tcagtccgta
     2761 tctgtctccg tcccagaagc cgtgcccctc gaccgcctgc cggccgcctc acggaccagc
     2821 cgccgtagca cggcgacgtg gcggaagaac tccttgaccg gctgcgccgg gctgcccacc
     2881 acatcggcgc cagccggcac atccgccatt accccggcct gcgcccctat gcgcgcccgg
     2941 cgcccgatcc gcagatggcc tgtcagcccc gcctggccag cgaccatgac gtggtcttcc
     3001 aggatcgtgg aaccggaaat gcctgcctgc gaaacgataa cgcagccgcg gccgaggcgg
     3061 acgttgtgcc cgatctgcac cagattgtcg aggcgggaac ctgcgccgat gtgcgtgtcg
     3121 tgcaacgaac cacggtcgat cgtcgtgttc gcgccgatct ccacgtcgtg ctccaggatc
     3181 acgcggccaa tctgcggcac ggtgaggaag gcttcactcg tgatcgcaaa cccgaaacca
     3241 tcctggccta tgcgggcgcc aggatagatc acgacgcggt cgcctaccaa tgcgtggctg
     3301 atcgtgacgt gcgatccaat cctgcagtca gctccgagca caacaccatc gccaatgacg
     3361 gcaaggggag cgatgcgcgt acgcgccccg atttcggccc cagcgccgat gacggcgagc
     3421 gcgccaatct cggcacttgg atcaatggcc gcaccgggcg cgaccacggc agaaggatgc
     3481 acgctgggat caagaggtgg caggggaaag aacagcgacg cgacgcgtgc ccaggcgaca
     3541 tagggctcgg tggtggtaac ggcgacggcc gtgccgggga cattggcgga catgtcggga
     3601 tgcacgatca ccactccggc ctgtgtggct gccagcgcct cgatgtactt gcggttgtcg
     3661 agaaaactca cctcgtccgg tcgcgccgtc tgcaagggcg ccaccccagc cagcataagg
     3721 cggcgggggg gcgcctcggc ctgcgcagca tcggcaaccg cagcaaggct atgcggcccg
     3781 ctacggcgga agaagcgcgg atcacctgca atcggcgacc aatccatgtg cccacaccgc
     3841 gcgtcggctc acggcttctt tgctgcggca ggcgtggccg gcttggcggg tggcgacttg
     3901 ttctccgagg ttgcagcggc cgctccggcc ggcggcttca tgctcattgg cgagacgcct
     3961 tctggcggga tcaccacctt caccagcacc ttgttgaggt cttcaacaac cgcgggcgtc
     4021 atgtcgaact ccggggtggc accgagaatt tgtgcgcgat tcagcacgac attgatgccg
     4081 cggctgatcg ccacttgctg cgtgaccatt tcgattgccc gatcgatctg cgccatcacg
     4141 tattggctgg cttcttggat cacccggttc cgctcaccga acacccggcg cgattccgtg
     4201 atgcggtcct gcagttcctt ctcacgcgtg cggatctgct cgggcgtaag cttcgcgcga
     4261 tcattggcca gcgactggtt caggttattc agcgtctgct gctctttctg cgcgtcctcg
     4321 ttcagcttct gacggcgggc gacgaattcc ttgtccgcct gctgaaaggc gctcgaggcg
     4381 cggaccacgt cggcaagcga caacacgccg acaatggctg ccggcggggt cggccctttc
     4441 ggcagcggag tcacctcggg cggcggtggc aattgcacct gcgcctgtcc ctcggcacct
     4501 ggctgaccgc cctcagccgg catcaggctt gtctccggag ctgtctgctc ggctggcgca
     4561 ccgatggatg gccgcggcgg cgccgcttgc gcggggcgtg gagcggctgc gcgcggctgg
     4621 ttggggacga accactgttg gttctgctgc gccgcagccg gctggaacag caaaacggcg
     4681 cccgtgatga cggatgcctg aaggatgcgg atttgggaca ctagaacctc gtaccgaaac
     4741 cgaaccggaa aatctgcgtc tggtcgtgcg gctgtttgat gacaaatggc gccaagtcaa
     4801 cattgagcag gccgaattgg gtgcgccacg agatgcccac gccggcacca agccgtgcgg
     4861 cgggcgaatc ggcgatctgg cacgcctgcc ccgtgctcgg ttcaacacat acgttattgt
     4921 tgcggaaact cgctccggtc aggccgccga tatcgacaaa ggcccggccg gaaatgccga
     4981 tatccggtga cacgggcaac gggaaccgca attccgtcga ctgagtccag ataaagcgtc
     5041 cacccagcgg atcgcccgtg acggcgtcat gtgggccggc accaccgatt tcgaaaccgc
     5101 gtagattgtc gccgcccagg tagaaccggt caatgatctg ctcctggacg ccttcgttat
     5161 aaaggtaacc aacactgccg gtgaacgaga ggccccagtc gctgttgccg gtaaagcgat
     5221 cgagcggaag gaagtactgg ccgttgacgt tgttgcggat gaactggctg ttgccaccaa
     5281 ggcccgcgat gtcggttccc caggacacca tatagccgga gtggggatca atcttgctgt
     5341 cgcgatgatc ataggtcgtg gactggctaa cctgcgacag caatgagtaa ccagcttcct
     5401 gggcaatgaa gaagctcgca ttcgacgcaa tgttgaacac tgttcggccg accagcgtgt
     5461 agctccatac ctgtcggagg tactcattga agtcatagcc cacacgggcg tcgaacccga
     5521 cacgcttctc gtcgtacggc tctgtcccaa ggaagttggt ttgcacgaag aacaggtcgc
     5581 caccaaccac catattgcga tcgaggaaat atgggtcggt aaccgaggct gtgatggaac
     5641 tgcgcctctg cgccagcaca ccgttgatgc cggcggaaat cccggtgccc accaggttct
     5701 tttcgctcag gccgacatcg agcagagcgc ccgcgtcggt cgagtagccg ccgcccagcg
     5761 tgagttcgcc cgtggccttt tcgtccactg tggtggtgag cacggtcttg tcggacgtcg
     5821 agccctgcga ctgggcgatg ttgacactgt tgaagtagtt cagatcctgc aggcgctgac
     5881 gtgacagacg cactgcctcc ccgttgtagg gatctttttc ctggagccgg aactcgcgcc
     5941 ggatcacctt gtctttagta cgcgtgttgc cgacgatgtc gatccgctcg acatagacgc
     6001 gcggcccttc gccgacatcg aaggcgagat caacggtccg cttgtcagga tgcttggaaa
     6061 tgagtggctt gacctcgacg aaggcatagc cgcggtcgtg cacgtccttt tccatcacct
     6121 cgacggtgcg gccaacggca tcaccatcgt accagtcgcc cacatcgagc tgcaggtccc
     6181 cgcgcaactg gtcacccgtg aggttgcgca gatgcgatgt gacttcgatc ttgcccacac
     6241 gataacgcac gccttcgctt atcgtgaatg tcaggaagaa gcctgagcgg tcgggagcaa
     6301 gttcggactt ggcatcgagg atattgaagt cgatgtagcc gttcttcagg tagaagcggc
     6361 gcaacaattc cttatcgaag ctcaaccggt cggggtcata ttcatcggag gtcgacagga
     6421 agcgccacca gcgcgattca cggctgttga tcacctccga cagccggtcc tcgctgaatt
     6481 ccttattgcc gacgaagact atcttgctga tcagggtgat actgccgtca ttgatctgga
     6541 acacgacatc gacgcggttc tggtcaagtc gaatgacctg cgcgttgacg gtggcgttgt
     6601 agtagccgtg cttggcgtaa atatcgagaa tgcgctggcg gtcctcttct gcaagcgcag
     6661 gcgtgaaaac ggcgcgcgac ttgatctgga gcaccggacg aagttggtcg tcggtcaact
     6721 tatggttgcc ctcgaacgca acgcggttca ccagcggatt ttccaccacg tgcactacca
     6781 gcgtgtcacc gtcacgattc aggcggacgt cctggaagag accagtcgcg tacagtgtct
     6841 tcagactgcg atcgaggcgg tcggcatcga acggatcgcc cgggcggacg agcatgtagg
     6901 acaggaccgt ggccgtctcg atacgctgat tgccatcgat cttgatggcg gcaatggtgc
     6961 cgccattgcg cactggaccg gtcgcggctg cgggctgggc cgcagtccga accgaaggcg
     7021 cggggcgcgg ctgggcgggc ctctgggccg aggccgatcc caccaggccg agaaccaaca
     7081 tgcaggcagc ggcaagccac gcggcgcgtc tgcgaatcaa acagcgtctc ccctcagcca
     7141 ggcgccatag caggcacgca ccgcagcagg ccatccgtat tgacggctcg cgaaggatac
     7201 ctgccgcctc ctacccatag cagtcaatgg cggcagcgag aatcaggcgg aaggccaagg
     7261 tggccctttc agttccacca tattgccttc cgggtccgga acatagatcg acggtccctg
     7321 accataggcg ccgtagcgcg actcgatcgg gccaggctcg cagcctttct gcctgagatg
     7381 cgcaatgatc gcctcaccgt cgaaaggctc gacctgtaga cagaagtggt cgaggttgcg
     7441 cgcctcctta cccgccggtg cgccgccctt ctttcccaat ggaccgttca ccggcaccag
     7501 atcgatcagg gcgtttccgg cgcgaagctg ccagaggccg atgttctcct ggaccttctc
     7561 cagggtacag ccgagcacgt cgacgtagaa gtgcagcatt gcccgcaggt cgacgacccg
     7621 cagcaccaga tggtccagtt gcgtgatccg cagcatggtc acctctccaa aaggtggacg
     7681 cgggagttca gcctcttgcc aggacggcgg caagtgccag cggcaggttt ttctccacag
     7741 acactgacat caccagttat ccggctcacg cggcccgccg gattcgctta agttccggcc
     7801 atgtcagcct caacgatcac acacggccat ctgcttgccg ggcgcatcgc ctatgcgcag
     7861 ctgagggcag gctttcgcag tggcatcgag ccggtgctgc tcgctgccgc agtgccggcc
     7921 cggcctggcg atcgcgtgct ggaggcaggg agcggcgctg gtgccacctt gctatgcctg
     7981 atggcgcggg tagccggcgt gcagacaacc ggcattgaaa aaagcgagca gctaacccgg
     8041 ctcgcgagac agaacgcaga cgcaaacggc tggcccgcca tgtctttgat cgccgccgat
     8101 attgccgaag ccgcgttcac tggttcgttc gaccacgcct gcgctaatcc accatatcac
     8161 ccggcggacg gcacggcgtc accagacgcc gcccgcgatt cggcgaaacg cgattcgccg
     8221 gggttgcttg cggtctgggc cagggcgttg ggccggtccc tcaagccacg cggcaccctg
     8281 acacttatcc ttccgacagc ggcgctgccg cgggcagtca cggcgctcgc ggacagtggc
     8341 tgcgcgccga cggctctgct gccgctatgg ccgaagctcg gccgccccgc aaagctggcg
     8401 ctgctgcgcg gcgtcaaggg tgggcggtcg cagttccggg tcatgccggg attgctgttg
     8461 cacacctccg ccggggatta cacaccggaa gccgatgcag tgctgcggga cggagcggcg
     8521 ctggcgattt aggccgtggt cgacttagaa ggagtcgtca ccggtgtgcg gatgccgcag
     8581 gtgccagagc cgttcatggg cggcaactaa gctttccatg ttgcggcggc gcgtaccgtc
     8641 aggcgtcagc gggcggcggg tcagcatcat ctccaaaatg tgcaggagct gctcggccac
     8701 ctcgaaatct gcctgatcac aggcgtgatg aaaggcgatc aaaatcttgt cc
//
LOCUS       DATGEM010000100         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1224781, whole genome shotgun sequence.
ACCESSION   DATGEM010000100 DATGEM010000000
VERSION     DATGEM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224781"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VKQ27_03215"
     CDS             complement(<1..1307)
                     /locus_tag="VKQ27_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLY87971.1"
                     /translation="MATAHLASLEPNPYPAAALCERGSDQRAVSIRASTASAIEMFQA
                     YPDIRLLPVVDEHGAPLGAIFEKDVRRILFNPYGHALLRNPAFGGTLGERVRPCPMAD
                     IATSVPDLLEIYAEAGGREGMILTHQGRLFGVIVNQALVRAAGLHEIEQMRRQAERLD
                     RVDRASTHFEAEAASLAETLGALAHSIDSRAHETAQRARVNHTHAATVASAAVQTGDS
                     MAMVADRSRSLAEALDQLHRDTDRAKIAAQEAVGQVAAGSARATELVATARSIETVLS
                     LIRSLVSKTGLLAINATIEAARAGEAGRGFAVVASEVRSLASQTRAAAEEIAGHIADV
                     HAAIDEVVAGHVGIETVIATVDTIARSVDQTVAAQQAVTVGIAEGAGQAATASAEIRD
                     NIGEISRSVQEAAVGSIEMQRTAGGLSEAATRLTRRVAAFVKE"
     gene            complement(1924..3450)
                     /locus_tag="VKQ27_03220"
     CDS             complement(1924..3450)
                     /locus_tag="VKQ27_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HLY87972.1"
                     /translation="MIEGDTSNGRVPLGHMWLRQHFGLSVPLPYTESYAIAGARRTEV
                     MGARAIELYPRRYATGDNVIANLRFALRHEPLDLGILVAAFEVMPPATIEAWVRAEPS
                     GAYARRAWFLYEHFTGRILDLSDVAFGNYVEALDPKKHVVTTRRNSPRHRVADNLLGT
                     PALCPTVRRTARLEAQIGVHIDAEARAIIERYDPAILARAVRYLYTKETRSSFAIEGE
                     SPNADRTERFVAALRSAASFDASDKAAIVRLQNAIVDPRYAASDWRGFQNFVGEMTMD
                     FREEVHFICPRPEDVPSLMRGWTALTARMLSGAVEPVVAAAVAAFAFVFIHPFEDGNG
                     RIHRFMVHHVLSRMGFSPDSLIFPVSAAIVRDQRRYDEVLESFSQPLFQAIRWNWTAE
                     REIIVSNDTAPLYRFFDATLLAEYLYDRVVDTVRTDLREELGFIAVFDRAFEAVRTIV
                     DMPDRRAGLLIRLLLQNNGRLSGAKRGQFPELSDAEVVRMETAVQQAIASERSEHGAD
                     "
     gene            complement(3624..3869)
                     /locus_tag="VKQ27_03225"
     CDS             complement(3624..3869)
                     /locus_tag="VKQ27_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87973.1"
                     /translation="MATGLLQPTLESALIVCWVVWLALSRKRPHLSVHGDGSRTSINS
                     ELRVVGEVVQDRARDLITADNQILYLKPPAFTSMGRR"
     gene            complement(3960..5180)
                     /locus_tag="VKQ27_03230"
                     /pseudo
     CDS             complement(3960..5180)
                     /locus_tag="VKQ27_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          988 a         1687 c         1778 g          984 t
ORIGIN      
        1 atctccttca cgaaggcggc gacgcggcgg gtgagacggg tcgccgcttc ggacaggccg
       61 ccggcggtgc gctgcatctc gatcgatccg accgcggcct cctgcacgct gcggctgatc
      121 tcgccgatat tgtcgcgaat ctcggcgctg gcggtggcgg cctggccggc gccctcggcg
      181 atgccgacgg ttactgcctg ctgcgcggcg acggtctggt cgaccgagcg ggcgatggtg
      241 tcgacggtcg cgattaccgt ctcgatgccg acatggccgg cgacgacctc gtcgatcgcg
      301 gcgtggacgt cggcgatgtg cccggcgatc tcctcggcgg cggcgcgcgt ctggctggcg
      361 agcgagcgca cctcgctcgc gaccaccgcg aagccgcgcc ccgcctcgcc ggcgcgcgcg
      421 gcctcgatgg tggcgttgat cgcgagcagg ccggtcttgg agacgaggct gcggatcagc
      481 gacagcaccg tctcgatcga tcgcgcggtc gcgacgagct cggttgcgcg cgcgctgccg
      541 gcggcgacct ggccgacggc ttcctgcgcg gcgatcttgg cgcgatcggt gtcgcgatgg
      601 agttggtcga gcgcttcggc gagcgagcgg ctgcgatcgg cgaccatcgc catgctgtcg
      661 ccggtctgca ctgcggcgct ggcgacggtg gcggcgtggg tgtggttcac gcgtgcgcgc
      721 tgtgcggtct cgtgagcacg cgaatcgatc gagtgggcga gcgcgccgag cgtttcggcg
      781 aggctggcgg cctccgcctc gaaatgggtg ctggcgcgat cgacgcggtc gagccgctcg
      841 gcctgtcgac gcatctgctc gatctcgtgg aggccggcgg cgcgcaccag cgcctggttg
      901 acgatcaccc cgaacagccg cccctgatgc gtaaggatca tgccctcgcg gccgccggcc
      961 tcggcataga tttcgagcag atcgggtacg ctggtggcaa tgtcggccat cgggcaaggc
     1021 cgcacccgct cgccgagcgt gccgccaaac gcgggattgc ggagcagcgc atggccatag
     1081 gggttgaaca ggatgcgccg cacatccttc tcgaagatcg cgccgagcgg cgcgccgtgc
     1141 tcgtcgacca cgggcagcag gcggatgtcc ggataggcct ggaacatctc gatcgcactt
     1201 gcggtcgacg cgcggatcga gacggcgcgc tgatcgctgc cccgctcgca cagggcggcg
     1261 gcgggatagg gattgggctc cagagaagca agatgggcgg tagccatgcc gccgctctac
     1321 gggagcacgg taaacgcgac cttaggattt gatgacaatc ctgtgacgat gcgatcaaaa
     1381 taacacccaa gccacggggc ggaaacgatt gcttgagccg tgccggatcg atcgggggct
     1441 tgtcgtcgga ggcgggcaga gcgctgcaac agcgccgcga tccatgctat ccgataatgg
     1501 tgccggggac ggaatcgaac tgcgcgaatt cagaaaaatt tctcaatata ttacgcgaga
     1561 taccgtcggc tcagataccg taaatccgat tggtgtggcg gtgagtttcc gttcggcagt
     1621 cggattttcg ccttcgacgc agccttagtc gagcatgaga gcgctgtgcg cccgaactct
     1681 tcacgcttgc agcttgatcg ctttcgcccg gtgggctgca agcggtaatt gaaggggggc
     1741 ggcgtatcgt cagatgctgc cagctgtccg gtctcaaacc gggttgcggt gaagaccggc
     1801 gacgcagcgg tcgaaatgcc ggcacataac gcccgcctcc attcgcactc tatccgtttc
     1861 cccaccgaga ggcggaacga tatgcgattt acgcagacga taatcgatct ggcacggtgg
     1921 ggttcagtct gcaccatgct cgctacgctc gcttgcgatc gcctgctgga cagccgtctc
     1981 catcctgacc acctccgcat cgctgagttc ggggaactgg ccgcgtttgg ctcccgacag
     2041 ccgcccatta ttctgaagca gcaggcggat cagcagacca gcgcggcggt ccggcatgtc
     2101 tacgattgta cgcacagcct caaacgcgcg gtcgaagacg gcgatgaagc cgagctcttc
     2161 gcgcagatca gtacgcaccg tgtcgacgac acgatcgtag agatattcgg caagcagcgt
     2221 cgcatcgaag aaacgataga gcggcgcggt atcgttgctg acaataatct cgcgctcggc
     2281 cgtccagttc cagcggatcg cctggaacaa tggctgcgag aagctctcca gcacttcgtc
     2341 atagcgtcgc tgatcgcgca cgattgcggc cgagacagga aagatcaggc tgtccgggct
     2401 gaaacccata cggcttagca catgatgcac cataaagcgg tggatgcggc cattgccatc
     2461 ctcaaagggg tggatgaaca cgaatgcgaa ggctgccacg gcagccgcga cgactggctc
     2521 caccgctcca ctcagcatgc gagcagtgag tgccgtccat cctcgcatca gtgagggcac
     2581 atcctcaggc cgcgggcaga tgaaatggac ttcctcgcgg aaatccatcg tcatctcccc
     2641 aacgaaattc tggaagccgc gccagtcgga ggcggcgtag cgcggatcga cgatcgcgtt
     2701 ctggagccgt acgatcgccg ccttgtcgga cgcgtcgaag ctcgctgccg aacgcagtgc
     2761 tgcaacgaaa cgctctgtac ggtcggcatt ggggctttcg ccctcaatgg cgaaggacga
     2821 gcgcgtttcc ttggtgtaga gatagcgcac tgcgcgcgcg aggattgcgg gatcatagcg
     2881 ttcgatgatt gcgcgcgcct cagcatcgat atggacgccg atctgggcct ccagccgggc
     2941 cgtgcggcgc accgtcgggc acagtgcggg cgtgccgagc agattgtcgg caacccgatg
     3001 gcgcggcgaa ttgcgccggg tcgtgacgac atgcttcttc gggtcgagcg cctcgacata
     3061 gttgccgaag gcgacgtcag acagatccag tatccggccg gtgaaatgct cgtaaagaaa
     3121 ccaggcgcgc cgggcgtagg cgccgctcgg ctccgctcgc acccacgcct caatcgtcgc
     3181 gggcggcatc acctcgaacg ccgcaacgag gatgccgaga tcgagtggtt catggcgcag
     3241 cgcaaagcgg agattggcga tcacgttatc gcccgtcgca tagcgtcggg gatagagctc
     3301 gatcgcgcgg gctcccatca cttccgtccg ccgcgcaccg gcaatcgcat agctttcagt
     3361 atagggcagc ggcacgctga gcccgaagtg ctgacgcagc cacatatgtc ccaacggtac
     3421 gcggccgttg ctcgtatcac cctcgatcat ggcaacggac ttagcagcgt ggtatttcga
     3481 ttactattgc ggaaaaataa ggaatatcgc ttataagaga gtcccattat accgacttcg
     3541 cgtacgttct gagcacgatc agccccggcg taccgggata gctctcgtcc ggcgcgttgg
     3601 tcagagcact ccgtcggtga tcgctaccgc ctgcccatgg aggtgaaggc gggtggtttg
     3661 agataaagga tctggttgtc ggcggtgatg agatcacggg cacggtcctg aacaacttcg
     3721 ccaaccaccc gaagctccga attgatcgac gttcgggatc catcgccatg gacggaaagg
     3781 tgggggcgtt tgcgggacaa tgcgagccat acgacccaac aaacgatgag cgcgctttct
     3841 aacgtcggct gcaacaggcc ggttgccatc aaaaatcggg cgacgggtcc gttagtgcag
     3901 cttccgtccg atagatgtcg cccgtcccac cccagggctc cgaatcgagg ttaacggagt
     3961 taagcgtcga gggcggcgag taagtattgg gtatcccatc cggcgcattt tctgcggagc
     4021 ctgatgctgg tggattttgg ggcgggaggt ttgttggttt ttctgcgcaa gcctgaacgc
     4081 tctgatgcga cggggtctgg ggcgatgcgg acgtggttga tggcgaagtg ccggacgacg
     4141 gccatggttt tggcgccatg gcctttgcgc aggcgggatt gatcgtcgcc gaagacgacg
     4201 tcgaggatcc agtggagttg gttttcgatg cccccatggc cgcgcacggc ctgagcggcg
     4261 gcctcggctc cgaatttggc cgaggtgata taatagcggt tttcgaagcg gcagcgatcc
     4321 ttgagttcgg ccttgctgtc gaccttgatg atggcggcga cgtcgggcag gcgcagttca
     4381 ccggggaagc ggcggtcacc gttcagccag tcgacctcac gcgccagcgt gacggtgcgc
     4441 tgctcgatgc ggccatggcc cttgccggtc tcggtttcgg tacgctcgag gctagcggcg
     4501 ggggcctgcg cgaacaagtc ctcgatttcc ttgcgcaggg tcggctgatt ggctttgacc
     4561 gccagcagat aatcggcgcc ggcgatcacg gatcgccttg gcgatggtgc cgttgcaggc
     4621 gatcgcatcg atcgtgacga ccgcaccctt caacccgccg ccttcggcca gccgctccag
     4681 tagcagcggg atcgcggtga tttcgttgct cttgtcggca accgcctcct gcccgagcac
     4741 cagccggctg gtggtggcga acgccgaaac aagatggagc ggcggctcgc cggcaccccg
     4801 atcatggctg cgccgcgagg tctttccatc gatcgccaca agctccggcc gatccggcca
     4861 gcaggcgcgg acccagtccg taaaacaggc cgaaaacagc accggatccg gctcatcagc
     4921 agcgtcagcc agcgccccgt cggaacaccg tgatgatggg gtaaaaatcg ccgcagaaaa
     4981 tcgagatgct gattacccca cagcccgatc gcctcatagc catcgcaatc cgcaatcgtc
     5041 ccgcacaccg ccagcagcaa cacttccgac agcggatagg ccaccttatg cgatggccgc
     5101 ggatcctcga tcgccgaaaa atgatccagc agccccctca gccgcgactt ctctccaaat
     5161 cccgcgtcca acccatccac acgcgcctcc tgccacagcg cgcgcatcga ttcagcattc
     5221 tctcaactcg taaagccatt aacctgattt cggtgccctg cagtttgggc tcgtccgctt
     5281 gacccagctt ggccgcagcg cgccgtgggc gtcttgtccg agagccggat gcagggaaat
     5341 ccgcatgttc gtttcgatga gcagggggtg gaaacggatc gtgtggtgag ccccacaggc
     5401 gagccgcacg acaccgcgcc acctcctgac tctacgc
//
LOCUS       DATGEM010000101         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1117252, whole genome shotgun sequence.
ACCESSION   DATGEM010000101 DATGEM010000000
VERSION     DATGEM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117252"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VKQ27_03235"
     CDS             <1..287
                     /locus_tag="VKQ27_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87974.1"
                     /translation="RMQQYEAKVVRDMFSNALRNSPLYINDPEAFRQAQRVWLSKWLA
                     AFGDVAETWIEIPEARVNPAYNPTGRAVLCWSIFYPDFNGVFCFIPYQAG"
     gene            complement(528..1925)
                     /locus_tag="VKQ27_03240"
     CDS             complement(528..1925)
                     /locus_tag="VKQ27_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87975.1"
                     /translation="MLSAWCYVVAWAATLADQLGHLPYAGSVSGVAILLFLILQFPKQ
                     RRYAQILFLALFAIGMIGVVLAAHPRELFLAGWQRGGSYAAFFLALTTLRDAAETSPL
                     VRRCGQHLVAQPPGRRYAALTAGGHVFGITLSYGAIDLLAAMVMRANTLEAAGGSAEL
                     QGIRSRRMLLAIYRGFCVMNCWSPINIMTAVVSTAVPAAPMRLLLPLAFVVAAGMVAL
                     GWIEDRLSGARLRTRGGARPVTTESWAIHGRIIALVVLVMLIAEATAFVFRISLVAGV
                     TLAVPVVALGWLIVQSWRFAGPVAQRLPGLLGRRARRFLLRIPTIRGEATVLGGSGFM
                     GVTIGNLLPGTGLTPLIATLPPIAIPMLVPVVLIAAGLLGLNPVALVALVGAVVPDPG
                     ALGVPPAVLAFACMLGWGLAVTMTPMSSSAIITARWADVSPWTVTSRWNASFTSSALL
                     LAWLAIAVAFVLWPR"
     gene            2007..2648
                     /locus_tag="VKQ27_03245"
     CDS             2007..2648
                     /locus_tag="VKQ27_03245"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLY87976.1"
                     /translation="MLFECNKTTTENIYKLLVSTVVPRPIAWVTTQDIDGTVNAAPFS
                     FFNAVSGNPPVVAIGIGGRGPGDVKDTGGNIRRTGEFVVNLVSNRLAEQMNITAIDFE
                     KSVNELSEAGLTTAPSSHVKPPRIAESPVSFECERLVIVEVGVDRAVILGKVVAIHVA
                     DECVMDKDRCYIDTPKLDLIGRMHGRGWYARTTDRFDMPRIDVADWPLRKAAE"
     gene            complement(2652..4220)
                     /locus_tag="VKQ27_03250"
     CDS             complement(2652..4220)
                     /locus_tag="VKQ27_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLY87977.1"
                     /translation="MNDFAGIIRAERPLFEGADWEFSTVQRVHDAIEKVALNDLGLDV
                     YPNQIEVISAEQMLDAYSSISMPLFYKHWSFGKHFARNEALYRAGMQGLAYEVVINSN
                     PCISYIMEENTATMQALVIAHAAFGHNHFFKNNYVFKQWTDAAGILDYLEFARGYIAH
                     CEERYGEGAVERLLDAAHALMTHGVHRYPRRRVADLRSEELRAKERREHDERVFNDLW
                     RTVPTKEGGKEDTSLDERRRALLQLPQENILYFLEKTAPRLMPWQREVLRIVRMIAQY
                     FYPQQQTKLMNEGCATYVHYNIMNRLHERGQLTDGAMVEFLRSHTNVVFQPDFDDPRY
                     SGINPYALGFAMMRDIERMCTEPTAEDQQWFPTLAGADPMDVLKDVWANYRDESFILQ
                     YLSPRMIREFGLFHVLDDANEPNLRVEAIHDERGYRRIQRVLAKQYDIAWTLPDIQVV
                     DVNLAGDRRLIVHHRALNRILLEERDARFVLQHLADLWGYDVLLKEVDPGTDSVLKEH
                     AATPRGGIVQPDGS"
     gene            complement(4217..>5276)
                     /locus_tag="VKQ27_03255"
     CDS             complement(4217..>5276)
                     /locus_tag="VKQ27_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HLY87978.1"
                     /translation="GDTIPRPQGGAGGGGAEGSPDGEGQDDFRFALSRDEFVDLFLED
                     LELPDLAKRRVVDTESLSWHRAGYSVTGSPANLALLRTVRNSLSRRIALKRPKPHELQ
                     ELRDEIAALSPDGEDSARRVELEEELARKMRRTGLIPYIDPLDVRYRKFEAMPKPVAQ
                     AVMFCLMDVSGSMTEHMKDLAKRFYVLLYIFLKRRYKHVDVVFIRHTHQAQEVDEDTF
                     FHSPETGGTVVSTALEEMQRVVDERYSPDSWNIYAAQASDGDNTASDNDKTAALLTET
                     ILPLCQYYAYLEVGRDSEHFPPGFIRRDSDLWQTYTTIIKSGAPMAMRKVGHRRDIYP
                     VFRELFAKKQPATAGAEE"
BASE COUNT         1039 a         1667 c         1663 g          907 t
ORIGIN      
        1 atcggatgca gcagtacgaa gccaaggtgg tgcgcgacat gttcagcaat gcgctaagga
       61 attcgccgct gtatatcaac gacccggaag cattccggca agcccaacgg gtctggctta
      121 gcaagtggct cgccgccttt ggcgacgttg ccgagacctg gatcgagata cccgaggcac
      181 gggtgaatcc agcctataac cctactggcc gcgccgtctt atgctggtct atattctatc
      241 ctgacttcaa cggcgtattc tgtttcatcc cgtatcaggc aggctgatcg tccagcggat
      301 gagcgacgtg ctcaacatgc atgctcccct cgagccaatc gagactgcaa tagggttgtt
      361 cgtgagcggc ccgcagcagc agccttgggt aaacgcgccc cacacgatca tgcgcaatat
      421 ctggactaca gtgtgatgac cggaagcggc gtcgccggag gtctcaatca cgcgacaaaa
      481 ccactacccg gagcgggatc agcgcgtcct tcagcgctca tcccgctcta gcgcggccac
      541 agcacgaacg cgacggcaat cgccagccac gcgagcaaca gagctgacga cgtgaaggaa
      601 gcgttccacc gcgacgtgac cgtccatggt gaaacgtctg cccagcgggc agtgatgatc
      661 gccgaactcg acatcggcgt catggtcacc gcgagacccc aaccaagcat gcatgcaaac
      721 gccaatacag ccggtggcac gccgagggcg ccggggtccg gcacgacggc accgaccagc
      781 gcgaccagtg caacagggtt gagcccaagg agaccggcgg cgatcagcac aaccggcacc
      841 agcatcggaa tggcgatcgg cggcagagtt gcgatcaggg gcgtcaggcc agtccctggc
      901 agtagattgc cgatggtgac gcccatgaac ccggaaccgc ccaggactgt cgcctcgcca
      961 cggatcgtcg gaatgcgcag caggaagcgc cgggcgcgac ggccgagcag cccaggcagg
     1021 cgttgcgcga cgggaccagc gaagcgccag gactgcacga tcagccagcc aagcgccacc
     1081 accggtaccg ccagggtgac accagccacc agcgagatgc gaaagacgaa ggccgtggcc
     1141 tcggcgatca gcatcaccag aaccacgagc gcgatgatcc ggccatggat cgcccaggat
     1201 tccgtcgtta ccggccgcgc gccgccccgc gtacgcaggc gggcacccga gaggcgatcc
     1261 tcgatccagc caagtgccac catcccggct gcaacaacga atgcgagcgg cagcagcaga
     1321 cgcatcggcg ccgcagggac agccgtagat acaacggcag tcatgatgtt gatcggactc
     1381 caacagttca tcacgcagaa cccgcgatag atcgcgagca gcatgcggcg tgagcggatg
     1441 ccctggagtt cggccgaacc gccggcggcc tccagcgtgt tggcccgcat caccatcgcc
     1501 gccagcaggt cgatcgcgcc gtacgagagc gtaatgccga acacatggcc gccagccgtc
     1561 agcgcagcgt aacgtcggcc gggtggctgc gccacgagat gctgaccgca gcggcgcacc
     1621 aatgggctgg tttccgcagc atcgcgcaag gtggtcaacg cgaggaagaa cgccgcgtag
     1681 ctgccgccgc gctgccaacc tgcaaggaac agttcccgcg gatgcgcggc cagcacaacg
     1741 ccgatcattc cgatagcgaa cagtgcgagg aacaggatct gcgcgtagcg gcgttgcttc
     1801 ggaaactgca ggatcaggaa cagcaggata gcgaccccgc tgacactgcc cgcatagggc
     1861 agatgtccga gctgatcggc cagcgttgcg gcccaggcga cgacgtaaca ccaagcgctc
     1921 agcacgcgcg catcctgccg cagggtggcg catctggaca ggggcagtgg cgccgcgtaa
     1981 cgctcgggga aacgcgaggg attgccatgc tgttcgaatg caacaaaacg acgaccgaaa
     2041 acatctacaa gctgctggta tccacggtgg tgccgcggcc gatcgcgtgg gtgacgacac
     2101 aggatatcga cggcacggtg aacgctgcgc cgttttcctt cttcaatgca gtgtcgggga
     2161 acccaccggt ggtggcgatt ggcatcggtg gccgtgggcc gggcgacgtg aaggacacgg
     2221 gcggcaatat ccgccgcacc ggcgagttcg tggtgaacct ggtcagcaac aggctggccg
     2281 agcagatgaa catcactgcg atcgacttcg agaagtcggt gaacgagttg tcggaagcgg
     2341 ggctgaccac cgcgccgtca tcgcacgtga agccgccgcg gattgccgag agccccgtct
     2401 cattcgaatg cgagcggctg gtgatcgtcg aagtcggcgt ggaccgcgcc gtgatcctgg
     2461 gcaaggtggt ggcaattcat gttgccgatg agtgcgtgat ggacaaggac cgctgctaca
     2521 tcgacacacc gaaactcgat cttatcgggc gtatgcacgg gcgcggatgg tatgcacgca
     2581 cgaccgaccg gttcgatatg ccgcggatcg atgtagcgga ctggccgctc cgaaaggcgg
     2641 ccgagtagcg cctagctacc gtcgggttgc acgataccgc cgcgcggcgt cgcggcgtgt
     2701 tccttcaata ccgagtctgt gcctggatcg acctccttca acagcacgtc gtatccccag
     2761 agatcggcca ggtgctgcag cacgaatcgt gcatcccgct cctccagaag gatgcgattc
     2821 agcgcgcggt gatgcacgat caggcgccga tcacctgcga gattcacatc caccacctgg
     2881 atgtccggca gcgtccacgc aatgtcgtac tgcttcgcca gtacgcgctg gatcctgcgg
     2941 tagccgcgct cgtcgtggat cgcctcgacc cgcaggttcg gctcgttcgc atcatccaac
     3001 acgtggaaca gcccgaattc gcggatcatg cgagggctga ggtactggag gatgaagctc
     3061 tcgtcgcggt agttcgccca gacgtccttc agcacatcca ttggatcagc gccggcaagc
     3121 gtggggaacc actgctgatc ctcggcggtc ggctcggtgc acatccgttc gatgtcgcgc
     3181 atcatcgcaa agccgagcgc gtacggattg atgcctgaat agcgcgggtc gtcgaagtcc
     3241 ggctggaaca cgacgttggt gtgcgagcgc aggaactcga ccatcgcacc gtcggtcaac
     3301 tggccgcgct cgtgcagccg gttcatgatg ttgtagtgaa cgtaggtcgc acagccttcg
     3361 ttcatcagct tggtctgctg ctgcggatag aagtactggg caatcatacg cacgatgcgc
     3421 agcacctcgc gctgccacgg catcagccgg ggcgccgtct tttccaggaa gtagaggatg
     3481 ttctcctgcg gcagctgcag cagcgcgcgg cggcgctcat ccagactcgt gtcttccttg
     3541 ccgccttcct tggttggcac ggtgcgccac agatcattga acacgcgctc gtcgtgctca
     3601 cggcgttcct tagcgcgtag ctcttccgag cgcagatcgg ccacacggcg gcgcggatag
     3661 cgatgcacac cgtgcgtcat cagcgcatgc gcggcgtcca gcaggcgctc gacggcgcct
     3721 tcgccgtatc gctcctcgca atgcgcgata tagccgcgcg cgaactccag atagtcgagg
     3781 atgccggcag catccgtcca ctgcttgaag acgtagttgt tcttgaagaa gtgattgtga
     3841 ccgaacgcag cgtgcgcaat caccagcgcc tgcatcgtgg ccgtgttctc ctccatgatg
     3901 tagctgatgc atggattgga gttgatcacg acttcgtagg cgaggccctg cataccggcg
     3961 cgatacagcg cctcattgcg ggcgaaatgc ttgccgaatg accagtgctt gtagaacaaa
     4021 ggcatgctga tcgaggaata ggcatcgagc atctgctcgg cgctgatgac ctcgatctgg
     4081 ttgggataga catccagccc gaggtcgttc agcgcgactt tctcaatcgc gtcatggacg
     4141 cgctggacag tgctgaattc ccagtcggcg ccttcgaaca aaggacgctc ggcgcggatg
     4201 ataccggcga aatcgttcat tcctcggctc ctgccgtggc cggctgcttc ttggcgaaca
     4261 gctcgcggaa cacgggatat atgtcgcggc gatggcccac cttgcgcatc gccatcggtg
     4321 cgccggactt gatgatcgtt gtgtaggtct gccaaagatc agagtcgcgg cggatgaacc
     4381 ctggcgggaa gtgctcggag tcgcggccga cttccagata cgcgtagtac tggcagagcg
     4441 gcaggatggt ctcggtcagc agcgcagcag tcttgtcgtt gtcggacgcg gtgttgtcgc
     4501 catccgacgc ctgcgcggcg tagatgttcc acgaatcggg gctgtagcgc tcatccacca
     4561 cccgctgcat ctcttccaga gcggtggaga cgacggtgcc gccggtttcc ggcgagtgga
     4621 agaacgtatc ctcatccact tcctgcgcct ggtgcgtgtg gcggatgaac accacgtcca
     4681 cgtgcttgta ccggcgcttc aggaaaatat agagcagcac atagaaccgc ttggcgaggt
     4741 ctttcatgtg ctcggtcatc gagccggaca cgtccatcag gcagaacatc accgcctgcg
     4801 cgaccggctt cggcatcgcc tcgaatttgc gataacgcac atccagcgga tcaatatacg
     4861 ggatcaggcc ggtgcggcgc atcttgcgcg ccagctcttc ctccagctcc acccgccgtg
     4921 cgctgtcttc accgtccggg ctgagcgctg cgatctcgtc gcgcagctct tgcagttcat
     4981 gcggcttcgg ccgcttcagg gcgatccggc gcgacaggct gttccgtacc gtgcgcagca
     5041 acgcgagatt cgcgggcgag ccggtgaccg aatagccggc gcgatgccag ctcaggcttt
     5101 cggtatccac cacccggcgc ttcgccagat ccggcagctc caggtcttcc aggaacagat
     5161 cgacgaactc gtcgcgcgac agagcgaagc ggaagtcgtc ctggccttcg ccatccgggc
     5221 tgccttccgc gcccccgccc ccggcgccgc cctgcggacg cgggatggtg tcgccc
//
LOCUS       DATGEM010000102         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1106943, whole genome shotgun sequence.
ACCESSION   DATGEM010000102 DATGEM010000000
VERSION     DATGEM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106943"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1211
                     /locus_tag="VKQ27_03260"
     CDS             303..1211
                     /locus_tag="VKQ27_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY87979.1"
                     /translation="MDETIRTAAISPLRQRLIDDMNVRRFSLATQHNYIRDVGRFATF
                     LGRSPDTATADDVRRFQIEQRDAGVSAPTMNSIVAALRFFFTHTIDRPDLARKLIRTV
                     HPRNLPVVLSRDEVTRLLNATTSLKHQAALSVAYGAGLRVAEVAALKVSDIDSERMLL
                     RVERGKGGRYRNAILPADLLTLLREWWKAGRQQGVMHAQGWLFPGQNTTKPISTRHLH
                     RIVVEAAQAAEITKRVGPHTLRHSFATHLLEDGVDIRVIQALLGHSKLDTTALYTKVA
                     TRTVRAVISPLDKLGIVATREAPPPA"
     gene            1216..2409
                     /locus_tag="VKQ27_03265"
     CDS             1216..2409
                     /locus_tag="VKQ27_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HLY87980.1"
                     /translation="MRASLEVADIFRAAGPAHRATHAGHLSLQHLKVMSAIEHCRTAA
                     LGGHVEACEDCGHSRIAYNSCRNRHCPKCQGAAARTWLAEREADLLPVEYFHVVFTVP
                     AEVADIALHNKAAVYDLLFQAASETMQTIAADPRHLGARIGITAVLHTWGSALTHHPH
                     LHMIVPGGGIALDGQRWVSSRPAFLLPVRVLGKLFRRLFLTKLTALHDAGRLAFFGRL
                     ASLADRRAFLRHLSPARHQRWVVYAKPPFAGPEAVLAYLSRYTHRVAISNSRLLSFNE
                     AGVTFRYKDYRRDGADRQRVMTLGTDEFIRRFLLHLLPRGFHRIRHYGLLSASARKAS
                     LARARELLAVASPPDDDLLEQSIGVHTPCPCCGGHMIIIETFARWCQPRAPPHRPKPT
                     GRTAP"
BASE COUNT          494 a          963 c          828 g          501 t
ORIGIN      
        1 ccgtcgcctt caaactatga cgcgagaacg gttgttgtcg ttggagctat cactgcgatg
       61 ctcggtcggg ttgcgccacg cgagccgttg aagggggagt gtgggtgcga gaacgcgatg
      121 tccggtttta ggatcggcca atccgtggtc cggcgcccgc aacgggtggt tgaacgaacc
      181 cgcttcgcgg gaagggtgcg gtgtgttggt gaacgagtag aggcccatct ggcatgactt
      241 gattgagcgc gggccggtgc gggctgagcg tttcggctct ttcaaccaag aggagccgac
      301 agatggacga gaccattcgc accgctgcaa tcagccccct gcgccagcgt ctgatcgacg
      361 atatgaacgt gcgccggttc tcgctggcga cacaacacaa ctacattcga gatgtaggac
      421 gcttcgcaac attcctgggg cgctcacctg atacggcgac ggcagacgac gtgcgccggt
      481 tccagatcga acagcgcgat gctggcgttt cggcgccgac catgaacagc atcgtggcgg
      541 cgttgcggtt cttcttcact cacaccatcg atcggcctga cctggcccgc aagctgatcc
      601 ggacggtaca tccgcgcaac ctgcctgtgg tgctgagccg agacgaggtc actcgcctgc
      661 tcaatgcaac caccagcctc aagcatcagg cggctctctc ggtcgcctat ggtgccggcc
      721 tgcgcgtagc cgaggttgcg gcgctcaagg tcagcgacat cgacagcgaa cgcatgttgc
      781 tcagggtcga gcgtggcaag ggcggccggt atcgcaatgc catcctcccg gcggatctgc
      841 tgacgctgct gcgcgagtgg tggaaggctg ggcggcagca gggcgtgatg cacgcacaag
      901 gctggctgtt cccaggtcag aatacgacca agccgatcag cacccggcat ctgcaccgca
      961 tcgtcgtcga agcagcgcag gcggcggaga tcaccaagcg ggtcgggccg cacacgctgc
     1021 ggcatagctt cgcgacccat ctgctggaag acggcgtcga tatccgcgtg atccaggccc
     1081 tgctcggcca cagcaagctc gacacaacgg cgctctacac caaggttgcc acacgcacgg
     1141 tgcgcgccgt gataagcccg ctcgacaagc tggggattgt tgccacgcgt gaggcgcccc
     1201 cgcccgcctg aggcggtgcg ggcctcactc gaggtcgccg atatcttccg tgctgccggt
     1261 cccgctcatc gggccaccca tgccgggcac ctgagccttc agcacctcaa ggtcatgtcg
     1321 gcgatcgagc attgccgcac ggcagccctg ggcggtcacg tcgaggcctg cgaagactgc
     1381 ggccactcgc ggatcgcgta caacagctgc cgcaaccggc actgtcccaa gtgccagggc
     1441 gccgccgcac gcacgtggct ggccgagcgc gaggccgacc tgctgccggt cgaatacttc
     1501 cacgttgtct tcacagtacc cgccgaggtc gccgacatcg cgctccacaa caaggccgcg
     1561 gtctatgacc tgctgttcca ggcggcgtcc gagacgatgc agacgatcgc tgcggatcct
     1621 cgccatctcg gcgcccgcat cggcatcacc gccgtgctcc acacgtgggg atcggcactg
     1681 acgcaccacc cccacctgca catgatcgtg ccgggcggcg gcatcgcact cgatgggcag
     1741 cgctgggtct cctcgcgacc agcgttcctt ctgccggtgc gcgtgcttgg caagttgttc
     1801 cgccgtctgt tcctcaccaa actgaccgca ctccatgacg ccggcaggct tgccttcttc
     1861 ggccgccttg catccctcgc tgaccggcgc gccttcctga ggcacctctc gccagcccgc
     1921 catcaacgct gggtggttta tgccaagccg cccttcgctg gccccgaagc agtgcttgcc
     1981 tatctctcgc gttacactca ccgcgtcgcc atctcaaaca gccggctgct cagcttcaac
     2041 gaggcaggcg tcaccttccg ctacaaggat tatcgccgcg atggcgcgga ccggcagcgt
     2101 gtcatgacac tcggtaccga cgagttcatc cgccgcttcc tgctccacct tctgccacgc
     2161 gggttccatc gcatccgtca ctacggtctg ctctcagctt cggcacgcaa ggccagcctc
     2221 gcgcgtgccc gcgaactcct ggctgtcgcg tcaccacccg atgacgacct gctggaacaa
     2281 tcgatcggtg tccatacgcc gtgtccatgc tgcggtggac acatgattat catcgagacg
     2341 ttcgcccgct ggtgtcagcc gagggcgccg ccgcaccggc caaaaccaac cgggaggact
     2401 gccccgtgac ccggcacgga ctgcaccaca ctccggccgc cgcaaccgtg cctcggccaa
     2461 tggcccagct tgtgcccaat gtcataaatt gcgatccgat ggtcgacttt gaccaccatt
     2521 ccaacatcag aaaccggtat accgcgcacc actgccacaa cggtgctacc cgccagcccc
     2581 gccgcagcgt ccgccaaacc aattccaacg acagtccaga aacaaattcc ccatagacta
     2641 cctgctcgtc gcgggttcgt tcatccacga ctttcgtacg cctccggcgc ccgaaactct
     2701 tcagctttct gccggtccgc ttacgggccg ggatggggct gttgtcagac attcagtccg
     2761 aggcaagtgc gggtctttga acgaat
//
LOCUS       DATGEM010000103         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1338534, whole genome shotgun sequence.
ACCESSION   DATGEM010000103 DATGEM010000000
VERSION     DATGEM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338534"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VKQ27_03270"
     CDS             <1..322
                     /locus_tag="VKQ27_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLY87981.1"
                     /translation="TPLKPVAAKPVPAPDKRVAVAAPTGKDMLVQLAAVRTEADAKSE
                     WERLNKRMPDLFAQRRPAISKTDHDGRTLWRVRTGGFADLTQAKDFCERVRAKGTGCT
                     VAEF"
     gene            340..1284
                     /gene="nagZ"
                     /locus_tag="VKQ27_03275"
     CDS             340..1284
                     /gene="nagZ"
                     /locus_tag="VKQ27_03275"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLY87982.1"
                     /translation="MTGTELAADEAALFRELPPAGVILFARNIQSPPQLRELTAQLRR
                     VLLPGVVLMVDQEGGRVARLRPPHWHLHPPASALVSLRAAWLTGALIGLDCANAGFDV
                     VAAPVLDLAIPGAHAVIGDRALASEPEQVARLGRAFAGGLLAAGIQPIGKHVPGHGRA
                     KIDSHLALPRVEANNLDADLLPFALNADMPWAMSAHIVYPAWDPALPATLSPTVISTI
                     IRHRIGFQGVLVTDDLAMKALSGSPAALAGQALEAGCDVALYCSGELAATADLLEQCP
                     PLTAPAAGRLAAARSLARRRLMPLNARSLADERDRLLA"
     gene            1281..2021
                     /locus_tag="VKQ27_03280"
     CDS             1281..2021
                     /locus_tag="VKQ27_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HLY87983.1"
                     /translation="MNSIILQIALAAIPAILAITLHEAAHGYAALALGDDTAKQAGRL
                     SLNPIRHIDRVGTIILPGFLLLFQLLTIGRVLFMFGWAKPVPVSAWKFKDPRRGMALV
                     ACAGPLMNFFLAWVAALLMPRPTDVVGNGFDYLDAAINQHPLVGLFLFYFMLTNLVLG
                     LFNLLPIPPLDGGRIAVGLLPLELARGWARMERFGIIVVVLVVFLLPHLIPGFDPFGQ
                     ALNTIVPRAISLLLRLAGHDIGGGDVRI"
     gene            2008..2769
                     /locus_tag="VKQ27_03285"
     CDS             2008..2769
                     /locus_tag="VKQ27_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HLY87984.1"
                     /translation="MSGSEEPASLVLRLEGFEGPLDLLLDLARTQKVDLVKISILALV
                     EQYLAVIEGARRVRLELAADWLVMAAWLTWLKSRLLIPEGADAEEGEQAADALAARLR
                     DLSAVRAAAVWLVGRPQLRHDVFARGAPEDHTEIDRSRLALDLSSLVRAYLQVMRQRV
                     RTRQYQPRLLTLWSVKDALQRLATLVGSLPDWTSLDDFLPEILGSPMERRAALASTLL
                     AGLEMARGGTVRLRQEEAFGPILVRRAPTGVEEGA"
     gene            2766..3353
                     /gene="scpB"
                     /locus_tag="VKQ27_03290"
     CDS             2766..3353
                     /gene="scpB"
                     /locus_tag="VKQ27_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLY87985.1"
                     /translation="MTEDEALRVAEALLFASAEPVGARALSQLLPEELDADTVLAALR
                     ERYAGRGVELVEAGGGMQFRTAPDLAPLLRKVIHIPRRLPRVAMETLAIIAYHQPLTR
                     PEIEEIRGTSLAQQTLDALLEAELIAPKGRRESPGRPTLWGTTPHFLAQFGLKDLREL
                     PRREDLLLEPPAPSISAETGEGGDVAPPPQPDPGH"
     gene            3379..3906
                     /gene="tatB"
                     /locus_tag="VKQ27_03295"
     CDS             3379..3906
                     /gene="tatB"
                     /locus_tag="VKQ27_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HLY87986.1"
                     /translation="MFDFAWSELGLIAVVAMILIGPKDMPVAIRSVTAWIRKARRMAA
                     EFQTHVDEMVREADLGDIRSSINEIRNFDIRGQIEKAVDSDGSIRASFAENPLEPSVT
                     SVEEIAVAESAVAELAPPSEAVAVAPLAPEPCVPHAPAFVPPNMARPLPPGAENLEAP
                     AFIPPDIARQQARAH"
     gene            3909..>4346
                     /locus_tag="VKQ27_03300"
     CDS             3909..>4346
                     /locus_tag="VKQ27_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HLY87987.1"
                     /translation="MADIEDDPINDKPMPLLEHLVELRRRLMWSIGAFIICFMVSYYF
                     SSRIYFFLAEPLANVLRAQGNPDPHLIYTQLYEAFFTRIKVAFFAGAFIAFPIIANQI
                     WLFVAPGLYRSEKRALLPFLLATPVLFLLGAALAYYFVFPFAWK"
BASE COUNT          660 a         1439 c         1415 g          832 t
ORIGIN      
        1 cacgccgctc aagccggtcg cggccaaacc ggtgcctgcg ccggacaaac gcgtggcagt
       61 agccgccccg accggcaagg acatgttggt gcagctcgct gccgttcgca ccgaggccga
      121 cgcgaaatcc gagtgggagc ggttgaacaa gcgcatgccc gacctgtttg cccagcgccg
      181 tccagcaatc agcaagactg atcacgatgg tcgcactctg tggcgtgtga ggactggcgg
      241 cttcgcggat ctgactcagg ccaaggactt ctgcgaacgc gtgcgggcga agggcacggg
      301 ctgcacggtg gccgaattct gaaggccgcc atcgtcggca taactggcac ggagctcgcg
      361 gccgacgaag cagcgttgtt ccgggaattg ccgccagcgg gcgttatctt gtttgcccgc
      421 aatattcagt cgccaccgca gttgcgcgaa ctcacggcgc agctgcgccg ggtcttgctg
      481 cctggcgtgg ttctcatggt ggatcaggag gggggccgcg tcgcccggct gcggccgcca
      541 cactggcatt tgcatcctcc ggcctccgct ctggtcagct tgcgcgccgc ctggctgacg
      601 ggcgcgctga tcggcctgga ctgcgccaat gctggcttcg atgtggtcgc agcacctgtg
      661 cttgacctcg ccatcccggg tgcgcacgcc gtcatcggag accgcgcgct ggccagcgaa
      721 cccgagcagg tcgcccgcct cggccgggcc tttgccggcg gcctgctcgc ggccgggata
      781 cagccaatcg gcaagcacgt tcccgggcat gggcgtgcca agatcgacag tcacctcgcc
      841 ctgccgcgcg tcgaagcgaa caacctggac gcagacctgc tcccgtttgc cttgaacgcg
      901 gacatgccct gggccatgtc cgcgcatatc gtctatcctg cctgggaccc ggcgctgccg
      961 gcgacattgt ccccgacagt gatcagcacg atcatccggc accgtatcgg cttccaagga
     1021 gttctggtga cagacgatct tgccatgaag gcgctttcgg gctcgcccgc cgcattggcc
     1081 gggcaggcgc tggaggctgg ctgcgacgtg gcgctctatt gcagtggcga acttgccgcg
     1141 acggcagatc tgttggagca gtgtccgcct ctgacagcgc cggcggcggg gcgcctggcg
     1201 gcggctcgtt cgctcgcgcg ccggcggctg atgcctctca acgcgcgatc gctggccgat
     1261 gaacgggatc ggctgctggc gtgaacagca tcatcctcca gattgctctg gccgcgatcc
     1321 ccgcgatcct ggccatcacg ctgcatgagg ccgcacacgg ctacgccgcg ctggcgctgg
     1381 gcgatgacac ggcgaagcag gctggccgcc tgtcgctgaa tccgatccgg cacatcgatc
     1441 gggtcggtac gatcattctg cccggcttcc tgctgctgtt tcagttgctg accattggcc
     1501 gcgtgctgtt catgttcggc tgggcgaagc cggtgccggt gtcggcctgg aagttcaagg
     1561 atccgcgccg cggtatggca ctggtggcct gcgctgggcc gttgatgaac tttttcctcg
     1621 cctgggtcgc ggcgctgctc atgccaagac cgaccgacgt cgtcggcaac ggcttcgatt
     1681 atctggacgc cgccatcaac cagcatcctc tcgttggttt attcctgttc tacttcatgc
     1741 tgaccaatct tgtgctggga ctgtttaacc tgctgccgat cccgccgctg gacggcgggc
     1801 gcatcgcggt cggcctgtta ccgctggaac tggcgcgcgg ctgggcgcgc atggagcgtt
     1861 ttggcatcat cgtcgtggtt ttggtcgtat tcctgttgcc ccatctgatc ccgggcttcg
     1921 acccgttcgg gcaggcgctc aacacgatcg tcccgcgcgc catcagccta ttgctgcggc
     1981 tcgccggcca cgatatcggc ggcggcgatg tccggatctg aagaacccgc cagcctcgtc
     2041 cttcgcctgg aaggcttcga gggaccgctt gaccttctcc tcgaccttgc gcgcacccag
     2101 aaggtggacc tggtgaagat ttccattctg gcgctggtcg agcagtacct ggcggtaatc
     2161 gagggcgccc gccgggtccg tctggaactc gcggcggact ggctggtgat ggccgcctgg
     2221 ctgacctggt tgaagtcccg gctgctgatt cctgagggcg cggacgccga ggaaggcgaa
     2281 caggctgccg acgcgctcgc cgcgcgcttg cgcgacctgt ccgcggtgcg tgccgccgct
     2341 gtgtggttgg tggggcggcc gcagcttcgc cacgacgtct ttgctcgtgg cgcaccggag
     2401 gaccacactg agatagaccg atcacgactt gcgctcgatc tttcctctct ggtgcgcgca
     2461 taccttcagg tgatgcgcca gcgcgtgcga acgcggcagt accagccacg cctgctcacg
     2521 ttgtggagtg taaaggacgc ccttcagcgc ctcgccacgc tggtcgggag tctgcccgat
     2581 tggaccagcc tcgacgattt tctccccgaa atactcggct cgccgatgga acgccgcgca
     2641 gcgcttgcca gcaccctgct cgcgggcctg gagatggcac ggggcgggac agtccggctg
     2701 cgccaggagg aggcgtttgg gccaatcctg gtgcggcgtg cgccgaccgg agtagaggag
     2761 ggcgcatgac ggaagatgaa gccctgcgcg tcgccgaggc actgctgttc gccagcgccg
     2821 aaccggtcgg cgcgcgtgcg ttgtcacagt tgctacccga ggagctggat gccgacactg
     2881 tgcttgcggc gttacgcgag cgctacgccg gccgaggtgt ggaattggta gaggccggcg
     2941 gcggcatgca gttccgcacg gcccccgatc tcgcaccgct cctgcgcaag gtgatccaca
     3001 tcccgcgccg ccttccacgc gtagcgatgg agacgctggc gatcatcgcc taccaccagc
     3061 cgttgacccg tccggagatt gaggagatcc gcggaaccag cctcgcgcag cagacgctcg
     3121 acgcgctgct ggaagccgag ctaattgcac cgaaggggcg ccgcgaaagt cctggccgcc
     3181 cgacgctgtg gggcaccact ccgcacttcc tggcgcaatt cggcctgaag gatctgcgcg
     3241 agctgccgcg ccgcgaagac ctgttgctgg agccgccggc cccttctatc tccgctgaaa
     3301 cgggagaggg tggagatgtg gcgccgccgc cgcagccaga ccctggccac tgaccactga
     3361 gcactgctaa ctgtccccat gtttgatttc gcctggtcgg agcttggcct gatcgccgtc
     3421 gtggcgatga tcctgatcgg cccgaaagat atgccggtcg cgatccgctc agtcacggcg
     3481 tggattagga aggcgcggcg catggccgcg gaattccaga cccatgtcga cgagatggtg
     3541 cgcgaagcgg atctcggcga catccgcagc tcgatcaacg agattcgtaa tttcgacatc
     3601 cgtggacaga tcgagaaggc agtggattcc gacggttcga tccgagcgtc cttcgccgaa
     3661 aacccgctgg agccgtcggt gacgtcggtc gaggagatcg ctgttgctga gtcggcggtg
     3721 gctgagctcg cgccgccatc cgaagcggtc gccgttgctc cgctggcgcc tgaaccctgt
     3781 gtgccccatg caccggcctt cgtgccgccg aacatggcac gcccccttcc gccgggcgca
     3841 gaaaacctcg aagcgcccgc ctttattcca cccgatatcg ctcgacagca ggcgcgcgcg
     3901 cactgaccat ggccgacatc gaagacgatc cgatcaatga caagccaatg ccgctgctcg
     3961 agcacctcgt tgagctgcgg cggcggctca tgtggtccat tggcgcgttc atcatctgct
     4021 tcatggtgtc gtactacttt tccagccgca tctacttctt cctggccgag ccgctggcga
     4081 acgtgctgcg tgcccagggc aatccggatc ctcacctgat ctacacgcag ctctacgagg
     4141 cgttcttcac gcggatcaag gtcgcgttct ttgccggtgc gttcatcgcg ttcccgatca
     4201 tcgccaatca gatctggctg ttcgtcgcgc ctggcctcta tcgcagcgag aagcgcgcct
     4261 tgttgccgtt tctgctcgcg acgccggtgc tgttcctgct gggtgcggcg ctggcctact
     4321 attttgtctt ccccttcgca tggaag
//
LOCUS       DATGEM010000104         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_426264, whole genome shotgun sequence.
ACCESSION   DATGEM010000104 DATGEM010000000
VERSION     DATGEM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426264"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1514
                     /locus_tag="VKQ27_03305"
     CDS             84..1514
                     /locus_tag="VKQ27_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HLY87988.1"
                     /translation="MTEPFILLIPLIAAAALAFIPNYRLSARGNVAASGITFVAALLL
                     LLDRPSTGRFLAVDDLNIVFILLNTFVGFTTSAFSASYIGHELETRRLTPSYLRFYHA
                     MFQVMLFGMNLALLANNIGLMWVAVELATLTTVMMVGIYRTHEAIEAAWKYFILGSVG
                     IALALFGTILVYLAARPVIGEGLDAMVWTTLLVRAHDFDPALMNIAFVFLLLGYGTKV
                     GLAPMHAWLPDAHAEGPTPISAVLSGLLLNVALYALLRFKLLMSANPGAIAPGPLMIA
                     LGLLSLIFAAFMLYRRRDIKRLFAYSSIEHMGITVFAFGMAGPLGNFAGLLQMTMHSL
                     TKSAIFFSVGHVAQVKGTQRISEMGGLTVTHPLLGWSLIAGVAAIAGLPPFGVFTSEF
                     LVVSSTFGQQPVLALLLVFGLLVSFGALFLRVTGVAFGEPRGPTAPVTASYVPMFAHL
                     ALVLTAGIWLPGPLVAWFQHVAALLG"
     gene            1511..2905
                     /locus_tag="VKQ27_03310"
     CDS             1511..2905
                     /locus_tag="VKQ27_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HLY87989.1"
                     /translation="MTLVDTGAWQEATHRLAAGEATLLSLWGEPGRVHMALFDTSVSV
                     LTLECSDATYPSVGAQHAPAIRLERAARDLYGLEPIGLPDTRPWLEHGPSPTTYPFLP
                     VEGEGLHQIPVGPVHAGIIEPGHFRFTADGETIVRLEQRLGYVHKGIDHLLHGATIEQ
                     AARLSGRTSGDSTVAYAFAFASAIEAALGIEVPPRAVWLRGVMAELERIANHLGDFGA
                     ICNDASFSIMLAHCGMLRERALRTAATCFGHRLMMDCIVPGGTSVDLPPDGPSGIRML
                     VDEIRARFPPLVDLYDKTASLQDRTARTGILSTELARRFGAGGYVGRASGRAFDARKN
                     FAYPPYDQLHFTVPVLQEGDVNARVWIRIKEIDASLTLIEQMLDQLPHGDVRIPIDAA
                     GPGEAIAVTEAFRGDLLVWLRLDDEGRIDRCHLRDASWFQWPLLEAAIEGNIVADFPL
                     CNKSFNCSYSGHDL"
     gene            2967..>3419
                     /locus_tag="VKQ27_03315"
     CDS             2967..>3419
                     /locus_tag="VKQ27_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HLY87990.1"
                     /translation="MLRTLLQGLRPPVTETAPLATLGVELNRAAQRRLGRSLAIREVD
                     AGSCNGCELEIHALNNAFYDLERFGLRFVASPRHADVLMVTGPVTRNMRQALERTWRA
                     TPDPKWVVAVGDCAAGCGVFANSPACIGSVAQVVPVDLVIQGCPPTPTQ"
BASE COUNT          536 a         1196 c         1012 g          675 t
ORIGIN      
        1 atcggcattt tcctgttccg catccgcgag cgtttcgaca cggtggacct ggaagcgctg
       61 gaccagttcc gcggggaacg gcgatgacgg aacctttcat ccttctcatt ccactgatcg
      121 cggcggcggc gctcgcgttc atccccaatt accgcctgtc ggcgcgcggc aatgtcgccg
      181 ccagcgggat aacgttcgtc gccgccctcc tgctcctcct cgatcgtccc tccactggca
      241 gattccttgc tgtcgacgac ctcaacatcg tcttcattct gctcaacaca ttcgtcggct
      301 tcaccaccag cgcgttcagc gccagctata tcggccacga gctggaaacg cgccgcctca
      361 cgcccagcta tcttcgcttc tatcacgcaa tgttccaggt catgctgttc ggcatgaacc
      421 tggcgctgct cgccaacaac atcggcctga tgtgggtcgc agtcgaactc gccacgctta
      481 ccaccgtgat gatggtgggc atctaccgca cacatgaggc gatcgaggcc gcatggaaat
      541 acttcatcct cggcagcgtc ggcatcgcgc tggcgttgtt cggcacgatc ctggtttatc
      601 tcgccgcgcg ccccgtcatt ggcgagggcc tcgatgccat ggtctggacc accttgctgg
      661 tccgcgcgca cgacttcgac cccgctctga tgaacatcgc cttcgtcttc ctgctgctgg
      721 gctacggcac caaagttggc ctcgcgccga tgcacgcctg gctgcctgac gcacacgccg
      781 aaggccctac gccgatctcc gccgtgctgt ccggcctgct gctcaatgtc gcgctgtatg
      841 cgctgctgcg attcaagctg ctgatgtccg ccaatcccgg cgcaatcgcg ccgggcccgc
      901 tgatgatcgc ccttggcctg ctgtcactga ttttcgccgc gttcatgctc tatcgccggc
      961 gcgacatcaa acgcctgttc gcctattcgt cgatcgagca catgggcatc acagtcttcg
     1021 ccttcggcat ggccggacca ttgggcaact tcgccggtct cttgcagatg accatgcaca
     1081 gcctgacgaa gtcggcgatc ttcttctcgg tgggccacgt cgcacaggtg aaaggcacgc
     1141 agcgcatttc cgagatgggc ggcctaactg tcacccaccc gctgctcggc tggagcctga
     1201 tcgccggcgt tgccgcgatc gccggcctcc cgccgttcgg cgtgttcacc agcgaatttc
     1261 tggtcgtcag ttccaccttc gggcaacaac cggttctggc gctgctgctg gtgttcggcc
     1321 tgctggtatc gttcggtgcc ttgttcctgc gcgtgaccgg tgtggcattc ggcgagccgc
     1381 gcggccccac ggccccggtc accgcctctt acgtgccgat gttcgcacac ctggcactgg
     1441 tgctgacggc cggcatctgg ctgccgggcc cactggtcgc ctggttccag cacgtcgcgg
     1501 cgctgctggg atgacgcttg tagatacagg cgcgtggcag gaagcaacgc atcgccttgc
     1561 cgcgggcgag gcaaccctgc tcagcctttg gggcgagccc gggcgcgtcc acatggcgct
     1621 gttcgacacc agcgtgagtg tcctcaccct tgaatgctcc gacgcaactt atccgtcggt
     1681 cggcgcgcaa cacgcgcccg ccattcgcct ggaacgcgcg gcccgcgacc tatacggcct
     1741 ggagcccatc ggcctgcccg atacgcgccc gtggctggag catggcccca gccccaccac
     1801 ctatccattc ctgccggttg aaggcgaagg cctgcatcag attccggtcg ggcccgtcca
     1861 cgccggcatc atcgagcccg gccatttccg tttcactgcc gatggcgaga ccattgtccg
     1921 cctggaacag cgtcttggct acgtccacaa gggcatcgac cacctgctgc atggcgcgac
     1981 catcgaacag gcagcgcggc tctccggtcg aacttccggc gatagcaccg tcgcctatgc
     2041 gttcgccttc gcgagcgcca tagaggcggc gcttggcatc gaggtgccac cacgcgccgt
     2101 ctggcttcgc ggcgtgatgg cggagttgga gcgcatcgcc aaccacctcg gcgacttcgg
     2161 cgcgatctgc aacgacgctt cattcagtat catgctggcc cattgcggga tgctgcggga
     2221 gcgtgcgttg cgcaccgccg caacctgttt cggccatcgg ctgatgatgg actgcatcgt
     2281 gccgggcggc acttctgtcg atctgccgcc cgatggcccg agtggcatcc gcatgctcgt
     2341 cgacgagatc cgcgccaggt ttcctcccct tgtcgatctg tacgacaaga ccgcatcgtt
     2401 gcaggatcgg accgcacgca ccggcatcct ttccactgaa ctggcacgcc ggtttggcgc
     2461 cggtggctat gtcgggcgcg cctccggccg cgcgttcgat gcccgcaaga acttcgctta
     2521 tccgccctac gatcaactgc acttcacggt gccggtcctg caggaaggcg acgtcaatgc
     2581 ccgtgtctgg atccgcatca aggagatcga tgccagtctg accctgatag aacagatgct
     2641 cgatcaactg ccgcatggcg acgtgcgcat cccgatcgac gcagccggac ccggtgaagc
     2701 catagccgtc acggaagcat tccgcggcga cctgttggtc tggctacgcc tggatgatga
     2761 agggcggatc gaccgctgcc atctgcgcga cgcatcgtgg ttccagtggc cgctgctcga
     2821 agccgcgatc gaggggaaca tcgtcgccga cttccctctc tgcaacaagt cgttcaactg
     2881 ctcctattcg ggacacgacc tgtgacaacg ccccctgtca ttgcgagcgc agcgaagcaa
     2941 tctcgcgcca gtacggtgcc ccttcgatgc tgagaacgct cctccaaggc ctccgccccc
     3001 ctgtcaccga aaccgccccg cttgcgacgc tcggcgtcga gttgaaccgc gcagcgcagc
     3061 gccggcttgg acgtagcctc gcgatacgcg aagtggacgc cggctcctgc aacggctgcg
     3121 aactggaaat ccacgcgctg aacaacgcgt tctacgatct ggagcgcttc gggctgcgct
     3181 tcgtcgcttc gccgcgccac gccgatgtgc tgatggtcac cggcccggtg acgcgcaaca
     3241 tgcgccaggc gctggaacgc acgtggcgcg ccacgccgga tccgaaatgg gtagtcgccg
     3301 ttggggattg tgccgccggt tgcggcgtct tcgccaacag ccccgcctgc atcggatcag
     3361 tggcccaggt ggtaccggtt gatctggtga tccagggctg cccgccgacg ccaacgcag
//
LOCUS       DATGEM010000105         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_542693, whole genome shotgun sequence.
ACCESSION   DATGEM010000105 DATGEM010000000
VERSION     DATGEM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542693"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VKQ27_03320"
     CDS             complement(<1..721)
                     /locus_tag="VKQ27_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLY87991.1"
                     /translation="MAYAELQVASAFSFLRGASSPEELAATASVLGLSAISITDLNSV
                     AGVVRMWDAAREVGIRLVVGTRLSFRDDTPDLLCYPQDRAAWGRLTRLLTVGKGRVPH
                     GDTSEAAKATNRCFLHYDDLLEHGEGLLLLAVAPDRLDEGFRADLQRLKADFGGRAFL
                     AANRRYRGDDARRLRAMAELPLPMVATNDVLYHLPNRRQLQDVMTCIRLGCRIDQAGL
                     ALQPNAERHLKSPQEMERLFGA"
     gene            complement(728..2230)
                     /locus_tag="VKQ27_03325"
     CDS             complement(728..2230)
                     /locus_tag="VKQ27_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLY87992.1"
                     /translation="MTRRILSVWLPHWPITRLSRNTPVSPDRPLVTIETIRGVRHLVA
                     VGKAGEAHGLFRGQTLTAARAVCPALVPVDADPEADETTLEKLAAWCERYTPMVAPDP
                     PDGLWLDITGCAATFPNEGELVRDLGAQLERNGVPCRIAAAGTPGAAWALARGEAGAG
                     EEGLADLPITCLRLEAGVVAGLRRLGLRTVGDLLRIPRAQITARFGAMPVLRLDQALG
                     EIEEAIDWPRQPLEWHERLAFAEPIGTPEDLARALGQLAERLCLRLGEQGKGGQRFVA
                     RFFRMDDVAPGIAVSTALPVRDPAYLTRLLCEKLETVDPGFGIEVITLEAESVAPMGV
                     TQTQLMAEPDVSDGLAGVVDMLSNRLGADRVSSVVQYASHVPERSVRRAPPLPVKQPS
                     WVNDPASPRPIRLLRRPEEIDVIAPVPDDPPIRFNWRGVAHRVRAAAGPERIAAEWWR
                     GKRSGGQPEMDLIRDYYRVEDSDGARFWIFRAGLHGEEQAPRWYLHGLFG"
BASE COUNT          459 a          892 c          624 g          353 t
ORIGIN      
        1 aagcgccaaa caaacgctcc atctcctgcg gcgatttcag atggcgctcg gcattgggct
       61 gcaacgcaag ccctgcctga tcgatgcggc aaccaagacg gatgcaggtc atcacgtcct
      121 gcaattgccg ccgattgggc aaatgataga gcacgtcgtt ggttgccacc atcggcaatg
      181 gcaactccgc catcgcgcgc aggcgacgcg catcatcgcc acgatagcgg cggttcgctg
      241 ccagaaacgc acgcccacca aaatccgctt tcagccgctg gagatctgcc cgaaatccct
      301 catccagccg atccggtgca acagccagca gaagcagccc ttcgccgtgt tccaacagat
      361 cgtcgtaatg cagaaaacag cgattggtgg ctttcgccgc ttcgctggta tcgccatgcg
      421 gcacacgccc cttaccaaca gtaagcagcc gcgtcagccg cccccaagcc gcacgatcct
      481 gcggataaca cagcaggtca ggcgtatcgt cgcggaacga cagtcgagtt cccaccacca
      541 gtcggatgcc gacttccctc gccgcatccc acatccgcac cacgccggcg acactgttga
      601 gatcggtaat gctgatggcg gacagcccca gcacggacgc cgtcgctgca agctcttccg
      661 gcgaagatgc gccgcgcaag aacgagaatg cgctcgccac ctgaagctcg gcatatgcca
      721 cggcaactca cccaaacagc ccgtgcaggt accagcgcgg cgcctgctct tccccgtgca
      781 acccagcccg gaaaatccag aatcgggcac cgtctgaatc ttccacccga tagtaatccc
      841 ggatcagatc catctccggc tgcccgccgg atcgcttgcc ccgccaccac tcagcggcga
      901 tccgctccgg ccccgccgcc gcgcgtaccc gatgcgccac acctcgccaa ttgaatcgta
      961 tcggtggatc gtccggcaca ggcgcgatca cgtcgatctc ctccggccgg cgcagcagac
     1021 ggatcggccg cggcgaagca ggatcgttca cccatgacgg ctgcttcacc ggcaaaggcg
     1081 gcgcgcgccg caccgagcgt tccggcacat ggctcgcata ctgcacaacg cttgacacac
     1141 gatccgcacc gagccggttg ctcagcatat ccactacgcc ggccagccca tcggacacat
     1201 cgggttcagc cattagctgg gtctgcgtca cgcccatcgg cgccacgctt tcggcttcca
     1261 gcgtgatcac ctcgatccca aatcctggat cgaccgtttc caacttctcg cacagcagcc
     1321 tggtcagata agcaggatcg cgcaccggca atgcggtgct caccgcaatg cctggcgcga
     1381 catcatccat ccggaagaac cgggcaacga aacgctgtcc ccccttgccc tgctccccta
     1441 accgcaagca cagccgctcg gccagttgcc ccagggcacg cgccagatcc tccggcgttc
     1501 cgatcggctc ggcaaaagcc agccgctcat gccattcgag cggctgacgc ggccagtcga
     1561 tcgcctcttc aatctctcca agcgcctgat ccagccgcag caccggcatt gcgccgaacc
     1621 gagcagtgat ctgagcccgc ggaatgcgca gcaggtcccc caccgtgcgc aagcccaacc
     1681 gccgcagccc tgccaccaca ccggcctcca aacgcaggca ggtgatcggc agatcggcaa
     1741 gcccctcctc acctgccccc gcctccccac gtgccagcgc ccaagccgcc cccggcgtcc
     1801 cagcagcggc aatccgacaa ggaaccccat tccgttccag ctgcgccccg agatcgcgca
     1861 ccagctcccc ttcattggga aacgtcgcgg cgcatccagt aatatccagc cacaacccat
     1921 ccggcgggtc gggagccacc atcggcgtat agcgttcgca ccaggcggcg agcttctcca
     1981 gcgtcgtctc atccgcctcc ggatcagcat ccaccggcac cagcgccggg cacaccgccc
     2041 gggcggcagt cagcgtttga ccgcggaaca gcccatgcgc ctcgcctgcc tttcctaccg
     2101 ccaccaaatg ccgcacgccg cggatggtct cgatcgtgac gagcggccta tccggcgaga
     2161 cgggcgtatt gcggctcagc cgcgtgatcg gccagtgcgg caaccacacg gagaggatgc
     2221 gtcgcgtcat ccgaagcctc catgatccat tcccctgggc gcccaccgcg cgcatgcgtc
     2281 aaagcaacat gccagcgggg cagtcccggc tccttgccgt cccgcacg
//
LOCUS       DATGEM010000106         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_651701, whole genome shotgun sequence.
ACCESSION   DATGEM010000106 DATGEM010000000
VERSION     DATGEM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651701"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..832
                     /locus_tag="VKQ27_03330"
     CDS             20..832
                     /locus_tag="VKQ27_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HLY87993.1"
                     /translation="MRLALTAAFAAIWASAFLSFAPAQAEAANPTAGLAAHRALYDLT
                     LANARDNDVIAARGTMGYEVIDACDGWAVRQRLAMTLTNTDGQDIQMVSDYATWESKD
                     GLKFRFHMKQTTDTAVTSQTDGEASLERPGGPGEVHYTVPDDDTKKLVPGTLFPMAHT
                     AAIIAAAREGKRFLALPLFDGTDDTGPEDSSIVILGWQQPQPNKYKFLAELPSTRVRL
                     AFFDHNSTSEVPTYQVGMRYWQNGVADDMQMDFGDFVMNAKLTDLTPLPHKC"
     gene            complement(820..1629)
                     /locus_tag="VKQ27_03335"
     CDS             complement(820..1629)
                     /locus_tag="VKQ27_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HLY87994.1"
                     /translation="MEGFWRHIRACNNANLPGDRLPFRIGTEQVGWVKPAFADRLAES
                     PEIRRTPEQVTLTDAASLQAIARRLSEAGCFRWRREAFDIRAQPDGPVLAQLDRGALP
                     SFGVLAVGVHVNGLVRRPEGLHLWVARRAANKALDPDKLDHLVAGGVPAGLSPAETMV
                     KEAEEEASIPASLARQAVEVGRITYAMERTEGLRRDLLHCYDLELPEAFVPYAADGEV
                     AGFELWPIERIMQTVRDTNDFKFNVNLVLIDLFLRLGLVAGSDAVSLRQHL"
     gene            1677..>2205
                     /locus_tag="VKQ27_03340"
     CDS             1677..>2205
                     /locus_tag="VKQ27_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLY87995.1"
                     /translation="MQRTRPGEAPLSILPRPQRSGMATIRSLLPYLWPAGDPGAKLRV
                     AAAMVLLVLAKLATVYVPVVYGRLVDALAPKDGGAMLAIPLALVLAYGGLRVGSAAFG
                     ELRDALFAKVQQRAVRTAALRTFRHLHALSLRFHLDRQTGALARAIDRGMAGIQSTLR
                     LAVFNVVPTLIELLMV"
BASE COUNT          385 a          754 c          682 g          384 t
ORIGIN      
        1 cccccagcgg agttcctcaa tgcgacttgc tctcacagcc gccttcgccg ccatctgggc
       61 ctccgctttc ctctccttcg cgccggcgca ggcggaagcc gccaacccaa cggccggcct
      121 ggcggcgcat cgtgcgctgt atgacctcac cttggcgaat gcccgcgaca acgacgtgat
      181 cgccgcccgc ggcaccatgg gctacgaggt gatcgatgcc tgcgatggct gggctgtccg
      241 ccagcgcctg gcgatgacgc tgaccaacac cgacggccag gacatccaga tggtttccga
      301 ttacgccacc tgggaatcca aggatgggct gaagttccgc tttcacatga aacaaacgac
      361 cgacacggcg gtgaccagcc agaccgatgg cgaagcctca cttgagcggc cgggcgggcc
      421 gggcgaagtg cactacacgg tgcccgacga cgacaccaag aagcttgtgc cgggaacgct
      481 gttcccgatg gcgcacaccg cggcgatcat tgccgcggcg cgtgaaggca agcgcttcct
      541 ggcgttgccg ttgttcgacg gtaccgatga caccggcccc gaggacagct cgatcgtcat
      601 tcttggctgg cagcagccac agccgaacaa gtacaagttc ctggcggaat taccgagtac
      661 gcgcgtccgg ctggcattct tcgaccacaa ttcgaccagc gaggtcccga cctaccaggt
      721 gggaatgcgc tactggcaga acggcgtggc cgacgacatg cagatggact tcggcgattt
      781 cgtgatgaac gcgaagctga cagatctcac gccgctgcct cacaagtgct gacggagtga
      841 gaccgcgtct gatccggcta ccaggccaag gcgcaggaac aggtcgatca gcacgaggtt
      901 gacgttgaac ttgaagtcgt ttgtgtcccg gacggtttgc atgatgcgtt cgattggcca
      961 cagctcgaag cctgctactt cgccatcagc ggcatacggc acaaaggctt ccggcagttc
     1021 caagtcgtag cagtgcagca ggtcgcggcg gagcccctct gtccgctcca tcgcgtaggt
     1081 gatgcggcca acctcgacag cctgccgcgc cagactcgcc ggaattgacg cttcttcctc
     1141 ggcctccttg accatggtct ctgccgggga aagtcccgcc ggcacgccac cagccacgag
     1201 gtggtccagc ttgtccggat ccagcgcctt gttggcggcg cgacgtgcca cccaaaggtg
     1261 caggccctcc gggcggcgga ccagtccatt cacgtgcacg cccaccgcca gcaccccaaa
     1321 actcggcagt gcgccgcggt cgagttgggc caacaccgga ccgtcaggct gggcccggat
     1381 atcgaacgcc tcgcgccgcc agcggaagca gccggcctct gacagccgcc gagcgatcgc
     1441 ttgcaaggat gcggcgtccg tcagggtcac ctgctccggg gtgcggcgga tctccgggct
     1501 ttcggccagc ctatcggcga atgccggctt cacccagccg acttgttctg tcccaatccg
     1561 gaatggcagc cgatcgccag ggaggttcgc gttgttgcag gcgcgaatat gccgccagaa
     1621 gccttccatc acggcctaac ctttcaaaca gcacatgtcc gtgccacatg ccggtaatgc
     1681 aacgcacccg gcctggtgag gcacctctct ccattctgcc acgaccacag cgctccggca
     1741 tggcgacgat acgctcactg ctgccctatt tgtggccggc tggcgatccg ggggcaaaac
     1801 ttcgcgtggc ggccgcgatg gtcctgcttg tgctggccaa gctcgcgacc gtctacgtgc
     1861 ccgtcgtcta tggccgcctg gtcgatgcac tggcgccgaa ggacggtggc gcgatgctgg
     1921 cgataccgct ggcgctggtg ctggcctacg gcggactgcg cgtcggctcc gccgcgttcg
     1981 gcgaactgcg tgatgcgcta ttcgcaaagg tgcaacagcg cgccgtgcgt accgccgcgc
     2041 tgcgcacgtt ccgtcatctg catgcgctga gcctgcgctt ccatctcgat cggcaaaccg
     2101 gcgcgctggc ccgcgccatc gaccgcggca tggccggcat ccagtccacc ctgcgcctcg
     2161 ccgtgttcaa cgtggtgcca acgctgatcg aactgctgat ggtca
//
LOCUS       DATGEM010000107         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_225346, whole genome shotgun sequence.
ACCESSION   DATGEM010000107 DATGEM010000000
VERSION     DATGEM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225346"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VKQ27_03345"
     CDS             <1..865
                     /locus_tag="VKQ27_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLY87996.1"
                     /translation="GAVGLDHLLIEQAIEDAVLLKNGCICCTVRGDIADTLDVLWERR
                     DAGEIPPFRRIAIETTGLADPAPVAHALLAEPGARYACRLDGIVTTVDALHGALQLDR
                     QPEARRQVAMADRVLLTKTDLATAAQIDDAAARIASLNPTALVRRITGGAALPSDVFD
                     LGPDEGRLEQWLRPLDGAGHHHLAFRHGEQIASVVLRHDRPIAWPALRHWLESILSLR
                     GDAILRLKGIVWLRDEEHPIILQGVHHVLHPPVPLDRPAQRNATRIVLITRGLSAKGL
                     RASFTAALARS"
     gene            936..1274
                     /locus_tag="VKQ27_03350"
     CDS             936..1274
                     /locus_tag="VKQ27_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87997.1"
                     /translation="MGRYVISVGMLVVCLTQPTQAAPPPDADPKLSPWFNDLAQPKTH
                     APCCSIADCRPVASRQSDGHYQAQIDGIWRTIPDEVILNRTDNPTGQAIACWTPSSGI
                     ILCFVPPPES"
     gene            1350..1520
                     /locus_tag="VKQ27_03355"
     CDS             1350..1520
                     /locus_tag="VKQ27_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY87998.1"
                     /translation="MTQLGRAIIAIMTGRIPRIPNAEFAVIDTWMEEDEYQAGFPNQW
                     NEAAGWRWWRPQ"
     gene            1570..1818
                     /locus_tag="VKQ27_03360"
     CDS             1570..1818
                     /locus_tag="VKQ27_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HLY87999.1"
                     /translation="MQSADITRVQAYLRKTFGTDRIHIDPPKTRSGPVEVRVGDEFIG
                     TLHRDTEDGEVSFSLHIMILEEDLPPVPAAVAPPPRRR"
     gene            complement(1821..>2129)
                     /locus_tag="VKQ27_03365"
     CDS             complement(1821..>2129)
                     /locus_tag="VKQ27_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY88000.1"
                     /translation="LDALLEQAGHAVRTYNSGQQLLDDTDLNDIACLVVDQKMPGMTG
                     LDLLRALDASGRTIPSLLITGAPDPVLAEEARALGAIDVLSKPIDFTRLLQLVDYTAQ
                     "
BASE COUNT          379 a          770 c          616 g          364 t
ORIGIN      
        1 cggcgccgtc ggcctcgacc atctgctgat cgagcaggcc attgaagacg ccgtgctgct
       61 gaagaacggc tgtatctgct gcaccgtgcg cggcgatatc gcggacacgc tcgacgtgct
      121 ctgggaacgc cgcgacgccg gcgaaatccc gccgtttcgc cgtatcgcga tcgaaaccac
      181 ggggctggcg gatcccgccc cggtcgcgca cgcgttgctt gccgaaccgg gtgcgcgcta
      241 cgcctgccgt ctcgacggca tcgtcaccac ggtcgatgcg ctgcacggcg cgctgcagct
      301 cgaccgccaa ccggaagcgc gccgccaggt cgcgatggcc gatcgcgttc tgctcaccaa
      361 gaccgacctc gccaccgccg cccagatcga cgacgccgcc gcccgtatag ccagcctcaa
      421 cccaaccgcc ctggtccgcc gcatcaccgg cggagccgcg ctaccctccg acgttttcga
      481 cctcggccct gacgagggcc gcctggagca atggctgcga ccgctcgacg gcgccggcca
      541 tcaccatctt gctttccgcc acggcgagca gatcgcctcg gtcgtgctgc gccacgatcg
      601 cccgatcgcg tggcccgcgt tacgccactg gctggaatcc atcctgtccc tgcgcggcga
      661 cgccatcctg cgcctgaagg gcatcgtctg gctgcgggac gaggagcacc cgatcatcct
      721 gcagggagtg catcacgtgt tgcatccgcc cgtcccgctc gatcgtcccg cacaacgcaa
      781 tgccacccgc atcgtcctga tcacccgcgg cctctccgca aaaggcctgc gcgccagctt
      841 caccgccgcg ctcgcgcgat cgtaagcatt tcttccgcac attgcccgct acgttgcgct
      901 agcatcatgc tgcaccgcat cacggagcga ccgccgtggg ccgatacgtc atctctgtcg
      961 gcatgctcgt cgtctgcctg acacaaccaa cgcaggcggc gccaccgcca gacgccgatc
     1021 cgaagctctc gccctggttc aacgacctcg cgcagccgaa gacgcacgcg ccatgctgct
     1081 ccattgccga ttgccgcccg gtcgcctctc gccagagcga cggccactat caggcgcaga
     1141 tcgatggaat ctggcgaacc atccccgatg aggtcatcct caaccgcacc gacaatccca
     1201 cgggccaggc gatcgcctgc tggacgccca gcagcggcat catcctgtgc ttcgtgccgc
     1261 cgccagagag ctgaccgctg aagttcacat tttcgccata atgtgagcaa acccggacat
     1321 cgtgcgttat atgcccaatc atgacagtag tgacgcaact cggcagggcc atcatcgcaa
     1381 tcatgacagg tcgaataccg cgcattccta atgccgaatt cgcggtcatc gacacctgga
     1441 tggaagagga cgaatatcag gcgggcttcc caaaccaatg gaacgaggcc gctggttggc
     1501 gctggtggcg cccccagtga cagcggtatc ctgggatgca acaaaccgct cgtcccagga
     1561 gagacagcaa tgcaatcagc cgacatcacg cgggttcagg cctacctgcg caagaccttt
     1621 ggcacggatc gcatccacat cgatccgccg aagacgcgca gcggtcctgt ggaagttcgc
     1681 gtcggcgatg agttcattgg cacgctccat cgcgatacag aggacggcga ggtttcgttc
     1741 tccctccata tcatgattct ggaagaggat ctgccgcccg tccccgccgc ggttgcgcct
     1801 ccgccgcggc gccgttgagg ctactgcgcg gtatagtcca ccagttggag caatctggtg
     1861 aagtcgatcg gctttgacag tacgtcgatc gctcccagcg cgcgcgcctc ttcggccagc
     1921 accggatcgg gtgctccggt gatcagcagg gacgggatcg tccgcccact cgcatcgagc
     1981 gcccgcagca gatccagccc cgtcatgccc ggcatcttct ggtccaccac gaggcacgcg
     2041 atgtcattga ggtccgtgtc atccaacagc tgctgtcccg agttgtaagt gcgcaccgca
     2101 tggcctgcct gctccagcag tgcgtcgag
//
LOCUS       DATGEM010000108        23121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_542529, whole genome shotgun sequence.
ACCESSION   DATGEM010000108 DATGEM010000000
VERSION     DATGEM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 23121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23121
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542529"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2805)
                     /locus_tag="VKQ27_03370"
     CDS             complement(<1..2805)
                     /locus_tag="VKQ27_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HLY88001.1"
                     /translation="MYRPEYEGDACGVGLVAATDGQPSRRVVAAAIDALKAVWHRGAV
                     DADGKTGDGAGIHVELPVRFFDDAIENGGHKPGSGRLGVGMVFLPRTDLAAQETCRTI
                     VESEIIDFGYTIYGWRQVPVDVSVIGDKAMQTRPEIEQIMIQGPRPDSADAAEFEKNL
                     YLIRRRIERKVIEAQIQGFYICSLSCRSIIYKGLFLAEELSAFYPDLLDERFVSRFAI
                     FHQRYSTNTFPQWWLAQPFRTLAHNGEINTVRGNTNWMKSHEIKMAAIAFGEHSEDIK
                     PLIPAGASDTAALDAVFEAICRAGRDAPTAKLMLVPEAWQGAADMPQAHRDMYAYYAS
                     VMEPWDGPAALAMTDGRWVVAGVDRNALRPLRTTLTADNLLIVGSETGMVVVPESTII
                     AKGRIGPGQMIAVDLDEGRLFSDSELKDRLAAEHPFGDYVKDFRELTDLPVPEGEHGA
                     RFERSELIRRQVAAGQTMEDMELILAPMVADAKEAIGSMGDDTPLAVISDKPRLISQF
                     FRQNFSQVTNPPIDSLRETRVMSLATRFGNLANILDTGPALANVLVLPSPVLTSTDWA
                     TLKAHFGASAAEIDCTFDPEGGPDALRGAIAEIRRQAEQAVREGRSELFLTDEHGGAD
                     RCGIQMVLAAAAVHTHLVRRGLRSYASINVRSAECLDTHYFAVLVGVGATTVNAYLTE
                     AAIADRQARGLFGDRSLEECLKRYRKAIDDGLLKIIAKMGIAVISSYRGGYNFEAVGL
                     SRALVNDLFPGMPAKISGEGYASLQLNALMRHEKAWDSAVATLPIGGFYRQRAGGETH
                     AYSATLMHLLQTSVSTDSYSTYLQFSRGVRDLPPVYLRDLLEFNWARETINIDEVEAI
                     TEIRKRFVTPGMSLGALSPEAHETLAIAMNRIGAKAVSGEGGEDKARYLPYENGDNAN
                     SVIKQVASGRFGVTSEYLVNA"
     gene            complement(2886..4346)
                     /locus_tag="VKQ27_03375"
     CDS             complement(2886..4346)
                     /locus_tag="VKQ27_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88002.1"
                     /translation="MAHDAMQKFVGVGQAYPEKRAPEIRAEDFAEITRAYLVEKAEEQ
                     AGRCSQCGVPYCSTHCPLHNHIPDWLRLTAEGRLREAYDLAASTSTMPEICGRICPQD
                     RLCEGNCVIEFAGHGAVTIGSIEKFITDTAWQEGWVEPIVPVRDRSDQSVGIIGAGPA
                     GLTAAEYLRGAGYDVHVYDRHDRAGGLLVYGIPGFKLEKEVVTRRIERLRASGIHFHE
                     SFEVGRDATLAQLRERHDAILVATGVYKARAIKAPGVGSPGIVKALDYLIASNRKSFG
                     DTVPDFETGVLNAHGKHVVVVGGGDTAMDCVRTAVRQGAKSVKCLYRRDRANMPGSQR
                     EVGNAEEEGVEFVWLSAPEAFDADQSIKAVHASKMRLGAPDASGRRAPEPEPGSEFRL
                     EANLVIQALGFDPEDLPQMFGAPELSVTRWGTLRIDHKTMQTSLPGVFAAGDIVRGAS
                     LVVWAVRDGRDVSERIHAWLKDKAEAAKAQPQTVAA"
     gene            complement(4504..5310)
                     /locus_tag="VKQ27_03380"
     CDS             complement(4504..5310)
                     /locus_tag="VKQ27_03380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLY88003.1"
                     /translation="MMILQPILLGIVEGLTEFLPVSSTGHLILAGALLGYDDAKWQVF
                     NIVIQLGAIMAIVVLYWRTFWAVLHGMARGRPQSWRFVRNILIAFLPAAAIGLAFHKR
                     IEALLLRPDIVCYALIAGGFAILLIEKLVKRHRIVGVASVPAPTTLGVGFIQCLAMIP
                     GVSRSGATILGALWLGVERRTAAEFSFFLAIPTMLGATVVELGKNPAALAAPGAVGWP
                     EIAIGFVTAFLVALVVVKAFVSIVSRHGFTPFAIYRIIAGTGALIWLMNK"
     gene            complement(5380..6315)
                     /locus_tag="VKQ27_03385"
     CDS             complement(5380..6315)
                     /locus_tag="VKQ27_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HLY88004.1"
                     /translation="MTELVTLFGGGGFLGRYVAQALLQRGVRVRIAERDPKRAFFVKP
                     LGGLGQTQFVAADITKADTVRRAVAGSHAVVNLVGILSGKMHLVHVEGARNVAEAAAA
                     AGARALVHVSAIGADATSSIDYARTKGEGEAAVRAAFPHATIVRPSIVFGAEDSFVNR
                     FAGMIQSAPVVPIIRPVTRFQPVFVADVAKAIANAALDPEAYAGRMFELGGPEILSMG
                     ELIRRTARMIGAEPTFLELPDSLSALIARFGFLPGAPITMDQWKMLQRDNVAAAGTDG
                     LADMGVQPTPIGAVAPSWLVRYRRAGRFGAVRKAS"
     gene            6450..6536
                     /locus_tag="VKQ27_03390"
     tRNA            6450..6536
                     /locus_tag="VKQ27_03390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6484..6486,aa:Leu,seq:cag)
     gene            complement(6791..7378)
                     /locus_tag="VKQ27_03395"
     CDS             complement(6791..7378)
                     /locus_tag="VKQ27_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88005.1"
                     /translation="MPAHDLEAAIILWVKSLLLDRRLVSGLIETDASALASLLQRASS
                     IAAQLETHHGGRTLVGKLAHQVRVEDEQIAIDIDCNALRRVLGLQTQSEAAILTITTP
                     AVRIRRGKDVKLVLANHDGDATERDPTLVALLGEARDVRDAALAAPDLSMKAIAVQMG
                     EWRTRATRQMRIAWLAPEIVEAISEGRHPPRLTPA"
     gene            7613..7735
                     /locus_tag="VKQ27_03400"
                     /pseudo
     CDS             7613..7735
                     /locus_tag="VKQ27_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            8756..9502
                     /locus_tag="VKQ27_03405"
     CDS             8756..9502
                     /locus_tag="VKQ27_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY88006.1"
                     /translation="MSWIDFFPGLAENGFSIKNKVFDEHCCSRVADDVNSEYERLVAS
                     GWRFCAGGRYTGHLNFHPGQYGQEMFAILKERGYIAAAEEFMGCPLNVSYIAGNINLS
                     GSLAQEIHQDFTPPEEAFIFNIALVTTRSNNGATAIVPQSHGTRYTYRTLHFTGTSRS
                     AQQIDGDPGDLLVRVGSLWHRGMPNRTPHHRPMLCICMVPADKPKPIDQVDERIAFSA
                     NRFYGKHARMRELAEIYLAKAFHYYRIIKS"
     gene            9656..10048
                     /locus_tag="VKQ27_03410"
                     /pseudo
     CDS             9656..10048
                     /locus_tag="VKQ27_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452059.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10239..10451
                     /locus_tag="VKQ27_03415"
     CDS             10239..10451
                     /locus_tag="VKQ27_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88007.1"
                     /translation="MDPAAAVKERVRVFDLQTGNQFVTGISDIMGGRRYNDWWIDERT
                     LATIKPAACRIEDEVVPIVGVNRRGN"
     gene            complement(10482..10805)
                     /locus_tag="VKQ27_03420"
                     /pseudo
     CDS             complement(10482..10805)
                     /locus_tag="VKQ27_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11256..11798
                     /locus_tag="VKQ27_03425"
     CDS             11256..11798
                     /locus_tag="VKQ27_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88008.1"
                     /translation="MRLGRSLAMVCGAAVALISAPRAHADSISIVAKGTINGACSITV
                     GQNFQSVNMTSATSASATAVVNCNTGFVTRATSANGALVSAGATSANFTNRLDYTLKL
                     AVPLDGGSTVSASCGAATLKAGQSSCVLSPAGGGLSSAGGIATNQTATLTASWTLPTS
                     PRPTAGSYFDTITLSITAMP"
     gene            11922..12386
                     /locus_tag="VKQ27_03430"
     CDS             11922..12386
                     /locus_tag="VKQ27_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88009.1"
                     /translation="MMAALAASTVAATPALAGTTATYTVNGSVDAACSISASGTLDFG
                     KLTDATGAFSNTAAPSSADGNAYCNQAATTVTVAHTDLTTSGTPTTGFTNDVTYTPVL
                     ITSDRTLTGNQTNATIGAFSSLTVKAQNAAASAKLVAGSYSGTISITLTPAT"
     gene            12927..13685
                     /locus_tag="VKQ27_03435"
     CDS             12927..13685
                     /locus_tag="VKQ27_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88010.1"
                     /translation="MKSLGTVDRIWLASALAFATASTAHATTVSPMVIDLQTTGRGVT
                     ANISVHNTGDKPMTMEVKPRALDVTDTGLIPGNGDTDDLLVMPPSALITPGSTLSFRV
                     QWIGDPAATASHHYFVGIDQLPVKLPEGQSAVQIVYNFDVLVNVGPTNGKAALAIKST
                     RIVDKDGKPAPAITVSNTGTTYGYMSQHKLRLVESDASGKSMFDKTYSGNDFEQLVGY
                     GIVATGQTRTMVLPVELPSRGGTLSATLLDEHAQ"
     gene            13675..16194
                     /locus_tag="VKQ27_03440"
     CDS             13675..16194
                     /locus_tag="VKQ27_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88011.1"
                     /translation="MRSELALLGQPMAVWLAPDAHGAISALFAAPGQMAGEVSSGQRP
                     ETAFAKPERINPTKRELRFIVPLTDGETYLGDLELAVSPQDMLSVDSVRLLDLMKPVL
                     QPAIRLRLGQLAGTSGRIDEPMLAKADIRLVYNAQNLSLDILIPVKDRQTRAMSFRGE
                     REAQADTFQPAPFSAYLNVYSAMDLVEAGASRGVVPPTAAFDAAIRARGVVVENEAYV
                     SGRSQEPAFRRTGSRVVYEDVRWQMRWTLGDIQLYARQFQDSPTVFGLGVSRIYSQID
                     PQREIRTSGAQSFGVISPSVIETFVNGRSVERRTFQPGNYTLQDFPLAQGANAVQLRI
                     EDSSGKIRTIDFSVYANQSLLAKGVTEFSLFGGVYSTATLTGFHYSRDWIANGFIYKG
                     LAEQLTAGLNFQANDRHRQAGTEILWGSPIGLAGLNLAANSSQAGDGLAVAMTYERLL
                     SQNGGAHSQSIRAAIEWRSSRFDLAELTSDPTRPRLRASAGYVITLGRNSFVAADLQY
                     ARDYRAAQSNYGGRLSGGFDLGERIAATAEFGINRGEPNRDTYVRVGLRMRFGGRGLV
                     QIDADSKGRTRANFSASHGGGVGAWQGSANLEQDANAVSFTANGTLSANRAELGVQES
                     VNWDKRSGQFSDARTTLRAAFAIAYADGSVALGRPITEAFMIAAPHRSLNGKAVYVDP
                     NEQSEAARSGQLGPALDGQLGAHSFRTLVYQVPDAPSGYDLGAGNVSISPPYRAGYRL
                     VIGSDYHLLVIGRLLDGGGEPIKLLAGRAVDLGNPKHPAVTLFTSRDGKFGAQGLRPG
                     RWRIDMPTDPPTSYEFDVTETSDGIVRVGDLHPVSSKKGGR"
     gene            16194..17129
                     /locus_tag="VKQ27_03445"
     CDS             16194..17129
                     /locus_tag="VKQ27_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88012.1"
                     /translation="MNRRLMAVCACIGAALGSGGAACAANCGTIAVSPSAPNFSSWNP
                     LNPANQQVPFVVTFARASASTKSVRLIFLDANSNAIPTRIGTTTGPRYQIVNTDSGAI
                     VSFPSGTSVTSQTVAVTQTSNGSANSFTINMSAVALANSSPTEDFIGGAAYTETLNYA
                     MQCFKANGQDNGVDSGVVSGLTLNLTIPKILSIVTASPVAIDFANFTSTSQQARISVK
                     STSTLNVAVSTSYGSRLVRNGAVPPFPTNSIIPYGMKFNGVAVAPSQPLVNQARAGVT
                     GSSYPLQLTLTDGIPSGKLAGSYSDTITLTLTPGQ"
     gene            17503..18246
                     /locus_tag="VKQ27_03450"
     CDS             17503..18246
                     /locus_tag="VKQ27_03450"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY88013.1"
                     /translation="MSNQVVLITGALTGIGRAAAIAFAKKGAKVVVSGRRDEVGQALA
                     KELQALGSEAEFINADVRKEDDVRALVDKAVARFGRLDVAVNNAGTEGDRGMVTDQTA
                     ESYAKTFDTNVLGVILSMKHQVRAMQAQGSGSIINISSTYGHEGAAGASVYVASKHAV
                     EGLTKSVALELAKSGIRVNAVAPGATDTGMLTRFTETPENKAALVTTVPMDRLGHTEE
                     IAEGIVFIGSDEASYITGHVLNIDGGKSH"
     gene            18423..18833
                     /locus_tag="VKQ27_03455"
     CDS             18423..18833
                     /locus_tag="VKQ27_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HLY88014.1"
                     /translation="MSDRPMKVSGPDHPIEIRPSPSRVVVTIGGQIIADTREALTLSE
                     ADYPSVQYVPRRDVDMAALMRSDHASWCPYKGEASYYSIPAGGERSHNAVWTYETPFP
                     AMVAIKDYIAFYPDRVDEIAERPGDAATLGAHGK"
     gene            18860..19534
                     /locus_tag="VKQ27_03460"
     CDS             18860..19534
                     /locus_tag="VKQ27_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HLY88015.1"
                     /translation="MKVAVVYHSGYGHTVKVAEAVARGAAGVEGAEVELIEAGVAPQR
                     WQTLDDADAIIMGAPTYMGSLSAPFKAFMDDSSHLQYAEKRWANKIAAGFTNGASRGG
                     DKQNSLIQLMTFAAQHQMHWVNLGLNYGNNRSVTNEEILNRDAYTLGMAAQADMDQGG
                     DVAPPASDLRTAEHLGRRVAEVAQELVAGRAALGRAVRPGIPGGSENQDPKRRGIVEA
                     GDVIAA"
     gene            19607..20491
                     /locus_tag="VKQ27_03465"
     CDS             19607..20491
                     /locus_tag="VKQ27_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY88016.1"
                     /translation="MDRLAALELFVRVVDTGSFSAVARDQGIGQPAVSKAVVQLEAWL
                     GVGLLLRSTRSVVPTEAGRIFYERARRMIEEAEEAVLAARGSGCGLSGKLRVSTSVCF
                     GRLHVIPHLPAFLAEHPDLQVELVLDDRQVDLVNEGIDVALRMGPMPDSSMVARRIAD
                     GRRVVVATPAYLERHGTPMSPVELASHQAILYSGRAGWTSWTFRKAEQAVSVELQGRL
                     KMTAAEGLREAVNCDLGFTIASEWNFWPELKSGKVVTLLEEWDLPSTHLSAVYPTGRL
                     ASTKARAFASFVERFLSS"
     gene            complement(20621..21685)
                     /locus_tag="VKQ27_03470"
     CDS             complement(20621..21685)
                     /locus_tag="VKQ27_03470"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HLY88017.1"
                     /translation="MRQLLVLLAMTTLIGAAPPGPRFAEVRPGVALVFPRDHGAHPDY
                     RTEWWYLTGWLDGPGETHRGFQITFFRIATGLGADSRSAFAPRQILFAHAALSDPAIG
                     HLLHGERIAREGLGIAAASRADADVAIDDWRLRRLPDGRFVAHVAGDGFALDLVFQPT
                     QPVLAEGDHGYSRKGPDPRSASRYYSLPQLVVSGTVTRSGRAEPVTGTAWLDREWSSS
                     YLPPGATGWDWAGLNFTDGGALMAFRIRRADGSTLWAGGTRRAADGDLTVLGPYDVAF
                     ELRQRWRSPRTGARYPIDPVLRIRLPDGIRTLPLRPLFPDQELEARAAGSPIYWEGAV
                     TTSGGRGYLELTGYASDLHL"
     gene            complement(21685..>23121)
                     /locus_tag="VKQ27_03475"
     CDS             complement(21685..>23121)
                     /locus_tag="VKQ27_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLY88018.1"
                     /translation="FAALGIAAALIGGLLPAYEAVRMAPMVALKNLGDAIDPRRRPRI
                     APALLLAFIGIGFALMPAVGGIALFGYGAVGLLLAAGIVAMPWLTRTLLAPLARHSFA
                     SPVTGLAVGRLWGAPSQAAVALSGIVASVGLMIAMAVMVASFRGSVDQWLNQLLSADL
                     YLSANGGTPFDSVTQARLAAVPGVGRIMFGAQHVLALTPNRPTVTLLVRDGAADAAPA
                     IGRTVAAPAGALAVRVSEPAARLYRLSVGSVMLLPLGAHRLPATVTAVYRDYARQEGA
                     ITIDGRSYVRLTGDTARGEAAIDLVPGADPGATMTALRGALPPAVADSVQIVPTRALK
                     TRALAIFDRSFAITYGLELVAILVGLAGVAATASAQTIARTREFGMLRHIGVTRRQII
                     AMLGMEGALLGLIGAIAGVALGCGLAQVLIRVVNPQSFNWTMETHLPVGLLSGVALAL
                     IVASALTAMLAGRRAVSADVVRAVREDW"
BASE COUNT         4364 a         7262 c         7314 g         4181 t
ORIGIN      
        1 cgcgttcacg aggtattcgc tggtcacgcc gaagcggccc gacgccacct gcttgatgac
       61 ggagttggcg ttgtcgccat tctcgtaagg gaggtagcgc gccttgtcct cgccgccctc
      121 gcccgacacg gccttggcgc cgatccggtt catcgcgatc gccagcgtct cgtgcgcttc
      181 cggggagagc gcgcccagcg acatgcccgg cgtcacgaag cgcttgcgga tctcggtgat
      241 cgcctcgacc tcgtcgatgt tgatcgtctc gcgcgcccag ttgaactcca agagatcgcg
      301 cagatagacc ggcggcagat cgcgcacccc gcgcgagaat tgcagatagg tcgaataact
      361 gtcggtcgac accgacgtct gcagcaggtg catcagcgtt gcggaatagg cgtgcgtctc
      421 gccgcccgcg cgctggcggt agaatccgcc gatcggcagc gtcgcgaccg ccgaatccca
      481 cgccttctcg tggcgcatca gtgcattgag ctggagcgag gcatagccct cgcccgagat
      541 cttggccggc attcccggga acagatcgtt gaccagcgcg cgcgacaggc ccaccgcctc
      601 gaaattatag ccgccgcgat aggaggagat caccgcgatc cccatcttcg cgatgatctt
      661 gagcagcccg tcgtcaatcg ccttgcggta gcgcttgagg cactcctcca gcgagcgatc
      721 gccgaacagg ccgcgcgcct gtcggtcggc gatcgcggct tcggtgagat aggcgttcac
      781 cgtggtcgcg ccgacaccga ccagcaccgc gaaataatgc gtgtcgaggc attcggccga
      841 gcgcacgttg attgaggcgt aggagcgcag gccccgccgc acgagatgcg tgtgcaccgc
      901 cgccgccgcc agcaccatct ggataccgca gcgatcggcg ccgccatgct cgtcggtgag
      961 gaacagctcg gagcgcccct cgcgcaccgc ctgctcggcc tggcggcgga tctcggcgat
     1021 ggcgccgcgc agcgcgtcgg ggccgccctc gggatcgaag gtgcagtcga tctcggccgc
     1081 gctcgcgccg aaatgcgcct tgagcgtcgc ccaatcggtc gaggtgagga cgggggaggg
     1141 cagcaccagc acattcgcca gcgccggccc ggtatcgagg atgttggcca gattgccgaa
     1201 gcgggtggca aggctcatca cccgcgtctc gcgcaagctg tcgatcggcg ggttggtgac
     1261 ttggctaaaa ttctggcgga agaactggct gatcagccgc ggcttgtctg agatgacggc
     1321 gagcggcgtg tcgtcgccca tcgatccgat cgcctctttg gcgtcggcca ccatgggcgc
     1381 gaggatcagc tccatgtcct ccatggtctg cccggcggcg acctggcggc ggatcagctc
     1441 ggaccgttcg aagcgtgcgc catgctcgcc ctccggcacg ggcaggtcgg tgagctcgcg
     1501 gaaatccttc acataatcgc cgaacggatg ctcggcggcg agccggtcct tgagctcgct
     1561 gtcggagaag aggcgccctt cgtcgagatc gaccgcgatc atctgccctg ggccgatccg
     1621 gcccttggcg atgatcgtgc tctccgggac cacgaccatg ccggtctcgg acccgacgat
     1681 cagcagattg tcggcggtga gcgtggtgcg cagcgggcgg agcgcgttgc ggtcgacacc
     1741 cgcgacgacc cagcggccgt cggtcatggc gagcgcggcc gggccgtccc acggctccat
     1801 caccgaggcg taataagcgt acatgtcacg gtgcgcctgc ggcatgtcgg cggcgccctg
     1861 ccatgcctcg ggcaccagca tcagcttggc ggtgggcgcg tcgcggccgg cgcggcagat
     1921 cgcctcgaac acggcgtcga gcgcggcggt gtcggaggcg ccggccggga tcagcggctt
     1981 gatgtcctcc gaatgctcgc cgaacgcgat cgcggccatc ttgatctcat ggctcttcat
     2041 ccagttcgtg ttgccgcgaa ccgtgttgat ctcgccattg tgcgccagcg tacggaaggg
     2101 ctgggccagc caccattgcg ggaaggtgtt ggtcgaatag cgctggtgga agatcgcgaa
     2161 gcggctgacg aagcgctcgt cgagcagatc gggatagaag gcgctgagct cctcggcgag
     2221 gaacagtccc ttgtagatga tcgaccggca cgacagcgag cagatgtaga agccctggat
     2281 ctgcgcctcg atcaccttgc gctcgatccg ccggcggatg aggtagagat tcttctcgaa
     2341 ctcggcggcg tcggcgctgt ccgggcgcgg gccctggatc atgatctgct cgatctcggg
     2401 gcgcgtctgc atcgccttgt caccgatcac cgagacatcg accggcacct gccgccagcc
     2461 gtagatggtg tagccgaaat cgatgatctc gctctcgacg atggtgcggc aggtctcctg
     2521 cgcggcaaga tcggtgcgcg gcaggaacac catgccgacg cccagccgcc cgctaccggg
     2581 cttgtgcccg ccattctcga tcgcgtcgtc gaagaagcgg acgggcagct cgacatggat
     2641 gccggcgccg tcgccggtct tgccgtcggc gtcgaccgcg ccgcggtgcc acaccgcctt
     2701 cagcgcatcg attgccgccg ccacgacgcg gcgcgacggc tgcccgtcgg tcgccgcgac
     2761 gaggccgaca ccacaagcgt cgccctcata ttcggggcgg tacatgccat gttcggcaag
     2821 gcgggcatgt tcgggggtgg ggaagtgggc catcgcctcg ctccgctgtt cgtcgaaagg
     2881 ggtcattatg cggccaccgt ctggggctgc gccttggcgg cctccgcctt gtccttgagc
     2941 caggcatgga tccgctcgga gacgtcgcgg ccgtcgcgca ccgcccacac caccaggctc
     3001 gcgccgcgca cgatgtcgcc ggcggcaaac acgccgggca gcgaggtctg catggtcttg
     3061 tgatcgatgc gcagcgtgcc ccagcgtgtc accgaaagct ccggcgcgcc gaacatctgc
     3121 ggcagatcct cgggatcgaa gcccagcgcc tggatgacga gattcgcctc cagccggaac
     3181 tcgctgccgg gctcgggctc gggcgcgcgg cggccggagg cgtccggcgc gccgagccgc
     3241 atcttcgagg catgcaccgc cttgatgctc tggtccgcgt cgaacgcctc gggcgcggag
     3301 agccagacga actcgacgcc ttcctcctcg gcattgccga cctcgcgctg cgagcccggc
     3361 atgttggcgc ggtcgcggcg gtagaggcac ttcaccgact tggcgccctg gcggacggcc
     3421 gtgcgcacgc agtccatcgc ggtgtcgccg ccgccgacca cgacgacatg cttgccgtgc
     3481 gcgttgagca cgccggtttc gaaatccggc acggtgtcgc cgaagctctt gcggttggac
     3541 gcgatcagat agtcgagcgc cttgacgatg cccggcgatc cgacgcccgg cgccttgatc
     3601 gcgcgcgcct tgtagacgcc ggtggcgacg aggatcgcgt catggcgttc gcgcagctgc
     3661 gccagcgtgg cgtcgcggcc gacctcgaag ctttcgtgga aatggatgcc cgacgcgcgc
     3721 agccgttcga tgcggcgggt gacgacctcc ttctccagct tgaagccggg gatgccgtac
     3781 acgagcagcc cgccggcgcg gtcatgccgg tcatagacat gcacgtcgta gcccgctcca
     3841 cgcagatatt cggcggcggt caatcctgct ggccccgcgc cgatgatgcc gaccgactgg
     3901 tccgaccgat cgcgcacggg aacgatgggt tcgacccagc cttcctgcca ggcggtgtcg
     3961 gtgatgaact tctcgatcga tccgatggtg accgcgccgt ggccagcgaa ctcgatcacg
     4021 caattgcctt cgcacagccg atcctgcggg cagatgcggc cgcagatctc gggcatggtc
     4081 gaggtcgagg cggcgagatc ataggcctcg cgcagccggc cctcggcggt gaggcgcagc
     4141 cagtcgggga tgtggttgtg cagcgggcag tgcgtcgagc aatagggcac gccgcattgc
     4201 gagcagcggc ccgcctgctc ctcggccttc tccaccagat aggcgcgcgt gatctccgcg
     4261 aagtcctcgg cgcgaatctc gggcgcgcgc ttctcgggat aggcctgtcc caccccgacg
     4321 aacttctgca tcgcgtcatg agccatcgcg taccctcctg ctagggacgc aaaagcatag
     4381 atttaggggt gagtcacgaa atgaaatggc gttaggtcag tatgtataac catttcattt
     4441 tgtttttcga cgctcccaga gaacgttcga tcgttcgatc gatttattaa gtccggatcg
     4501 gccctattta ttcatcagcc agattaaagc gccggtgcca gcgatgatcc gatagatcgc
     4561 gaagggcgtg aagccgtggc gcgagacgat cgacacgaac gccttcacca ccaccagtgc
     4621 gacgaggaag gcggtgacga agccgatcgc gatctccggc cagccgaccg cgccgggggc
     4681 cgcgagtgcc gcggggttct tgccgagctc gaccacagtt gcacccagca tggtggggat
     4741 ggcgagaaag aagctgaact cggctgcggt acgccgctcg acacccagcc acagcgcgcc
     4801 gaggatcgtc gcgcccgagc ggctgacgcc ggggatcatg gcaaggcact ggatgaagcc
     4861 gacgcccagc gtggtcggcg ccggcacgct ggcgacgccg acgatgcggt ggcgtttgac
     4921 gagcttctcg atcagcagga tggcgaaacc gccggcgatc agcgcgtagc agacgatgtc
     4981 cggccgcagc agcagtgctt cgatccgctt gtggaaggcg aggccgattg ccgcggcggg
     5041 caggaaggcg atcaggatgt tgcgcacgaa tcgccacgac tgcgggcggc cacgtgccat
     5101 cccgtgcagg accgcccaaa aggtccgcca gtagagcacg acgatcgcca tgatcgcgcc
     5161 gagctggatg acgatgttga acacctgcca cttggcgtcg tcatagccga gcagcgcacc
     5221 cgcgagaatc aggtggccgg tggaggagac gggcagaaat tcggtcagcc cctcgacgat
     5281 gccgaggagg atgggttgca ggatcatcat gaacacgaca ctcggctgcg agggcaggaa
     5341 aagaaaaggg ccggcgcatc ggccggccct agaaaaggat caggacgcct tgcggacagc
     5401 gccgaaacgg cctgcacgcc ggtatcgcac cagccaggag ggcgcgaccg cgccgatcgg
     5461 cgtgggctgc acgcccatat cggcgaggcc atcggtgccc gccgccgcga cattgtcccg
     5521 ctgcagcatc ttccactggt ccatggtgat cggcgcaccg ggcaggaatc cgaagcgggc
     5581 gatcagcgcg gacaggctgt cgggcagctc gaggaaagtc ggctcggcgc cgatcatccg
     5641 ggcggtgcgg cgaatcagct cgcccatcga caggatttcg ggtccgccca gctcgaacat
     5701 gcggccggca taggcctcgg gatcgagtgc cgcgttggca attgccttgg cgacgtcggc
     5761 gacgaacacc ggctggaagc gcgtgaccgg acggatgatc ggcaccaccg gcgccgactg
     5821 gatcatgccg gcgaagcgat tcacgaacga atcctcggcg ccgaacacga tcgaggggcg
     5881 cacgatcgtc gcgtggggga aggccgcgcg cacggcggct tcgccctcgc ccttggtgcg
     5941 ggcatagtcg atcgacgatg tcgcgtcggc gccgatcgcc gaaacatgca ccagcgccct
     6001 ggcgcccgct gcggcggcgg cttcggcaac gttgcgcgcg ccctcaacat ggacgaggtg
     6061 catcttgccg gagaggatgc cgaccagatt gaccacggcg tggctgccgg cgacggcgcg
     6121 ccggacagtg tcggccttgg tgatgtcggc cgcaacgaac tgggtctggc cgagcccgcc
     6181 cagcggcttc acgaaaaagg cgcgcttggg gtcgcgctcg gcgatccgca cccgcacgcc
     6241 gcgctgcagc agcgcctgcg cgacatagcg ccccaggaac ccgccaccgc cgaacagcgt
     6301 caccagctct gtcatcgcca gaccatcctt gtatcgaaaa gccaatctgc ggtcgccttg
     6361 cctccgcctc ggcaggaaaa caagaccgag cgaaagattg ccgttgacag ccccgctaac
     6421 ccgccgctat ccgccgcggc ctaccccgtg cccagatggc ggaattggta gacgcaccag
     6481 cttcaggtgc tggcgatcgc aaggtcgtgg aggttcgagt cctcttctgg gcaccactct
     6541 ctttatcgtc cgatctcagt gacttagcct tcgggctcct gccggaatgt ccccgcattc
     6601 tcggggcttt gcgaaggtga aataagggac gcggagccga gagacgctcg gctgccggcg
     6661 ttagcccgca aaaagggctc cagtctcccg gagccgtttt ccaaactcca cattatgccc
     6721 ggcgggatca ggagatcccg agtgcttgct tttgcgcatc ccagcacatc ggcaagtccg
     6781 tggcgagcag ctatgcaggg gtgaggcgtg gcggatgccg cccctcgctg atcgcctcga
     6841 caatctccgg ggcgagccag gctatgcgca tctgtcgggt cgcgcgcgtg cgccattccc
     6901 ccatctgaac cgcgatagct ttcatcgaca gatcaggggc tgcaagggcg gcatcgcgaa
     6961 catcgcgcgc ttcgcctagc agcgcgacca gcgtggggtc gcgctcggtg gcatcgccat
     7021 catgattggc caacaccagt ttcacatcct tgccccgccg gatacgcacg gcgggcgttg
     7081 tgatggtcag gatcgcggcc tccgattgag tttgaagccc gaggaccctc cgaagcgcgt
     7141 tgcaatcaat atcgatggcg atctgctcat cctcgacgcg cacctgatgc gccagcttgc
     7201 cgacgagcgt ccttccccca tggtgcgtct ccaactgagc cgctatcgac gatgcgcgct
     7261 ggagaagaga tgcgagcgca gatgcgtcgg tctcgatcaa cccgctcacc agccggcgat
     7321 cgagcagcag agacttcacc cacaagatga ttgcagcctc cagatcatgt gcgggcatgc
     7381 gctaagccgg tggctcgccc tcgcgtagtt cggatgggtg ggtaatataa tataatagcg
     7441 gtagcgtttg cccgacttca ccgcatggct cggtgacata cgcctgtcag gcgacatgca
     7501 attctggtcc cggtcgtccg ctgaaccctg cgcttggtgc agccgacggc gcttgaatgg
     7561 accacgcaac gcgtgtgggc agcttgccaa gcgccttctc ctgcagatga taggcgagcc
     7621 acgtgtttgc gcgatggtcc ggccgcctag cgtcgaggat gaagatcggc gtcgcatctg
     7681 ccgcgaacgc aaggttctga ccagcgagcg tatccggcat gaaaaccgga tcgaagcatg
     7741 ggaccggcct tacgagctga tcgatctcca cgagaaggtg gcgtcgtgaa gggccaagcc
     7801 ctgatcgcgc tgtgggcgtt tctgcacggg tttcacggtg ggctgcgatt tgagagggaa
     7861 ggctggcgtt tcgcccgatc gcaatgaaaa gacgtgtcga ccacgatctg cgagtgaaac
     7921 gagcggctga gtggcggcag tgagagtggg agataacttg gtcgaattgg cattgcaggt
     7981 tgtgaatgaa ccggcacggc ggcacgtcgt tccacttcgc gaacgcgacc cgctgtccct
     8041 ctcaaagagg atcgaaaagg tcctcgaata tcgctttctg gctgagtgtg cgtcgtcaac
     8101 ccagagccga gtccctggtg cacaagatgg gttagtcaat aattattgac caatgtaatc
     8161 tgtgctattt gtttaagtgt atttggaaat attgggctat atcgcttgaa ctatggaggt
     8221 gtgtcattgg tgtgtgccat acctgcgtta ccttgcggaa ggctgcgtta agacatggtt
     8281 gtgataatat aggtcatatt attttgcaga aatctctgaa ttaaccttat atgaggtttg
     8341 cgcgaaaggc aaaagcctct cggtgaatag gagattgtac tattctgcaa tatcatgaga
     8401 tatatagtga actatacgac ggttggcgtc agaaaggacg ccataccagt tcacgtccag
     8461 ttggagatgg agtagacatg attcgcgttt cgccgttgct gtccgatcat cgcctgctca
     8521 gcgtgccgtg ttttggcgcc acttatgcga atgtattcgt atataagaat tatatttatt
     8581 aataaactaa ataaaatatg tggatcatta cgattttgta aattagttca gaccgatttc
     8641 gtcatgtgac atttcgtcat ttcatttatg tattcaattg aatataacgt gatgaatgat
     8701 tcaattgatg ttctctatgt tggaaagaca actctctcca tggtagtaag aaatcatgag
     8761 ctggattgac tttttccctg ggctcgctga aaatggattt tcaattaaaa ataaagtgtt
     8821 cgatgagcat tgctgctcgc gagtcgcaga cgatgtaaac tccgaatatg agcggctagt
     8881 cgcatccggc tggcgtttct gcgcgggcgg acgttacact gggcatctga actttcatcc
     8941 gggccaatat gggcaggaaa tgttcgccat cctaaaggaa cggggttaca tcgccgctgc
     9001 agaagaattt atgggttgcc cgctcaatgt ttcttacatt gccggcaaca taaatctaag
     9061 cggaagtctt gcccaagaga tacaccagga cttcaccccg ccggaagagg ccttcatatt
     9121 caacatagca cttgttacga cccgttctaa caacggggcg accgcaatcg tcccgcaatc
     9181 tcacggcacg cgttacacat atcgcactct ccatttcact ggaacaagcc gctctgccca
     9241 gcagattgat ggtgatcccg gtgatttatt ggtacgggtc ggctcactgt ggcatcgtgg
     9301 catgcccaac cgcactccgc atcacaggcc gatgctttgt atttgcatgg tgcctgccga
     9361 caagcccaaa ccaatagacc aggttgacga aagaatcgcg ttctccgcaa accgcttcta
     9421 cggaaagcat gcaaggatgc gagaactggc cgaaatatac ttagctaagg ccttccatta
     9481 ctatcgcatc atcaaatcgt aatcactcca cttggagcct gatccgaaat taacccgtct
     9541 tatgcgccga ggggccgatt ttgggttggc gatcgtatcg taactatctg attcggttta
     9601 gagaagggtt tctcgcacca atcctccaga ccggacagaa agtaccacga ccgccatggc
     9661 agatagcggg ctacctttta cattcccgga gattcatcgt cggaaagtga ccgccgcgtt
     9721 cgatggcgga agaccgacgt cggatggtgg cgttttactg cttgctcaag cggagcggca
     9781 gctgggaatt gccagccgcc tggccggttg catcgctgat ccgcgcgatt agagtcgggc
     9841 agtccattct cccgccgaca ttctgcgtgc ccgcatactg gcgatttcct gcggctatga
     9901 ggatgccaac gatctcgaca tgctgcgcca cgatcccggg ttcaagctgg cgctcggcaa
     9961 gctgccggat ggaccggtcg gccttgccag ccaaccgacc atgtcgcgct gggagaatgc
    10021 accgaccacg cgcgagttgt ttcgcctgac cggcacgttg tagagattgc acatcgttcg
    10081 attgtgccac cgtcgatcgc cgtcgatcac cgtcgatgaa gccccatctt gcagggcagc
    10141 aggccatcat tatcgtagcc ccacatggca gagccgcgga tccaaggggc gcaacatcag
    10201 cattacgtgc cgcagcatct gctccgcggc ttctgagcgt ggatcctgcg gcggcggtga
    10261 aagaacgcgt gcgggtcttc gatctgcaga ccggcaacca gttcgtcacc ggcatctccg
    10321 acatcatggg agggcggcgc tacaacgatt ggtggatcga cgagcggaca ttggccacca
    10381 tcaagccggc cgcctgtcgg atcgaagacg aagtcgtccc catcgtcggt gttaatcggc
    10441 gcggaaatta ggctctgacg cagttttgat gatcctgggt gtcatgggga gccgatcagc
    10501 ctggcttcga gaaggtcgag tttgccgcgg ccgtacatct ggcgtttgac gagcttgagc
    10561 ttggtgatct gcccctcggt ttgcccgttg gaccaggacg tagcgatggc ggcgcgaacc
    10621 gcgttgctgt cgcgggcgac accgttggca aaggacgcga caagactggt gcgtccacgt
    10681 tctatccatg gggcgagatc ggcctccgcc ttgtgacgga tcatcgcgtg gaagctttcg
    10741 atgatcgcgc gagcttcaac cagtagtggc acgcctgcct ccactgcagc gacggtcaac
    10801 cccttcttca gcgcatcaag cagaccgccc tgctcaaatg ccgcgctggt agacccacgg
    10861 cttcccgaat cctggcatcg ctgcacgttt tgatccgtcg gtagatcgca atccagctcc
    10921 catgtctggg cgtcacgtcg accaattcgt ccacgccgcc tgataggcgc gattctccac
    10981 cgctcccgcg aatgttcgcc gtcatctttt ggcgaaaaat tctttaaatt cataactata
    11041 acaaacaaat tcaaataata gtttcgttca agcagtaaat ataatcgcaa tatttttcgt
    11101 acaatgaaag tattgaatgt atcaaattga gacaatattg tcgtttacca tgattgtcgt
    11161 gattgttcca ttagcagaat gccatgatac ccaatcaatc gggggatcaa ttcggtatac
    11221 cggatcgatt cttgtcaggg atcaagaggt gcctgatgag actggggcgt tctttggcga
    11281 tggtctgtgg ggcggctgtg gcgctgataa gcgcgccccg cgctcatgcg gactcgatca
    11341 gcatcgttgc caagggaacg atcaacggcg cctgctcgat cacggtcggc cagaatttcc
    11401 agagcgtcaa tatgaccagc gctaccagcg cctcggcaac agcggtggtc aactgcaaca
    11461 ccggattcgt gaccagggcg acatccgcca acggcgcgct ggtgagcgcc ggcgctacct
    11521 ccgccaattt caccaaccgg ctagactaca cgctgaagct ggcggtgccg ctggatggcg
    11581 gcagcacagt cagcgcgagc tgcggggcgg caacgctgaa ggcagggcag agcagctgcg
    11641 tgctttcccc ggccggcgga ggcctatctt cagccggggg catcgccacc aaccagaccg
    11701 cgacgctgac ggccagctgg acgctgccga ccagcccgcg ccccacggcg ggaagctatt
    11761 tcgacacgat caccctttcg atcacggcga tgccctgatg cgcggccgcg atcacacgcc
    11821 acagtctctg ccggggtttg ggcagactgt tccgggccat cagccggatt ttaacggtta
    11881 aatgcgaatg aaacaggaaa ggactgaaaa tgaggaaatg gatgatggcc gccctggcgg
    11941 cttcgaccgt ggcggcgacg cctgctcttg ccggtaccac tgccacctac accgtgaacg
    12001 gcagcgttga tgcggcctgc tcgatcagcg cgagcggcac gctcgatttc ggcaagctca
    12061 ccgacgctac tggggcattc agcaacaccg ccgcgccgtc gagcgctgac ggcaacgcct
    12121 attgcaacca ggcggcgacg accgtgacgg ttgcccacac cgacctgacc accagcggca
    12181 cgccgacgac cgggttcacc aacgatgtga cctatacccc agtgctgatc acgtcggacc
    12241 ggacgctcac cggcaaccag actaacgcca cgatcggcgc cttctcgagc ctgactgtca
    12301 aggcgcagaa cgccgctgct tcggcaaagc tggtcgctgg cagctattcg gggacgatca
    12361 gcatcacgct gacgccggcc acctgaaaat ctgggcggac aggacggggt gcgactcgtg
    12421 aaattcaatg ggaccatcgt ttccgtcgtt ctggcattgc tggtcggcgt gggatcttct
    12481 cctgcgtcgg ccggtacgac caaatcggta agcgtaactg gttcggtcga ccatgcttgc
    12541 agtctcagcg caaccagtgt cggccttcag gtgacccggt cgggcggcac catcacgaca
    12601 agcttcagcc caacgacggt tttggcatcg tgcaactctt ccgccggtgg aaggctcggg
    12661 gtcagttcca cccggctgca actatcgacc aatccgaata cgaaggttga ttatacgctc
    12721 tccgtttccg gctggggtac ccctgccatc acctacgcga cgtcagccac actgccgccg
    12781 ccagcgacca gatcgaatgc agcggcgggc aacgtttcgc tttcattttc ctgttcggtg
    12841 ggctgtacta ataacaatat tagtaataat tcgacatata atgccactat aatgctgagc
    12901 atgagcgcga atccctgaga ggaattatga aatcgctcgg aaccgtggat aggatttggc
    12961 tcgccagtgc gctggctttc gccacagcaa gtactgccca cgccaccacc gtctcgccaa
    13021 tggtcatcga cctgcagacg acggggcgag gggtcaccgc caatatctcg gtgcacaaca
    13081 ccggcgataa gccgatgacc atggaggtca agccacgcgc gctcgatgtc accgataccg
    13141 ggctgatccc gggcaatggc gataccgacg atctgctcgt tatgccgccg agcgccctga
    13201 tcacgcctgg gtcgacgcta agctttcgcg tgcagtggat cggtgatcca gccgccaccg
    13261 ccagccacca ttattttgtc ggcatcgacc agctgccggt caagctgccc gaggggcagt
    13321 cagccgtgca gatcgtctat aatttcgatg tgctcgtcaa cgtcggcccg acaaacggca
    13381 aggcagcgct ggcgatcaag tcgacgagga tcgtcgacaa ggacggcaaa cccgcaccgg
    13441 cgatcactgt tagcaacact ggcaccacct acggctacat gagccagcac aagcttcgcc
    13501 ttgttgagag cgacgccagc ggcaagtcga tgttcgacaa gacctacagc ggcaatgatt
    13561 tcgagcagct ggtaggctat ggcatcgtcg cgaccggaca gacgcgcacc atggtgctgc
    13621 ccgtcgaact gccgtcgcgc ggtgggacgc tgagcgcgac gctgctcgac gagcatgcgc
    13681 agtgaactag ccctccttgg ccagccgatg gcggtgtggt tagctcccga cgcccacggg
    13741 gcaatctccg cgctgttcgc cgcgccgggc cagatggcag gcgaggtgtc gtcgggccag
    13801 cggcctgaga ccgcgttcgc caagcccgag cggatcaatc ccaccaagcg cgagttgcgc
    13861 ttcatcgtgc cgctgactga tggcgaaacc tatctcggcg acctcgaact ggccgtctcg
    13921 ccgcaggaca tgctgtcggt ggattcggtg cgactgctcg atctgatgaa gccggtgctg
    13981 cagccggcaa tccgcttgcg gctgggccag cttgccggga cgagcggccg gatcgacgaa
    14041 ccgatgctgg cgaaggcgga catccgcctt gtctacaacg cgcagaacct gtcgctcgac
    14101 atcctcatcc cggtgaagga tcggcagacg cgagcgatga gctttcgcgg cgaacgcgaa
    14161 gcccaggccg acacgtttca gccggcaccg ttcagcgcct atctcaatgt ctattcggcg
    14221 atggatctgg tcgaggcagg cgcctcgcgt ggggtggtgc cgccgactgc cgcgttcgat
    14281 gccgcgatcc gtgcccgcgg ggttgtcgtc gagaacgagg cctacgtctc agggcgcagc
    14341 caggaaccgg cattccggcg cacgggcagc cgcgtcgtct atgaggacgt gcgctggcag
    14401 atgcgctgga cgctcgggga tatccagctc tatgcgcgcc agtttcagga cagcccaacc
    14461 gtgtttggtc tgggcgtgtc gcgtatctac agccagatcg atccacagcg cgaaatccgt
    14521 accagcgggg cccagtcgtt cggtgtaatc agccccagcg tgatcgaaac cttcgtcaac
    14581 ggccgcagcg ttgagcgcag aaccttccag ccgggcaatt acaccttgca ggatttcccg
    14641 ctggcacagg gggcgaacgc ggtccaactg cgtatcgagg attcgagcgg aaagatccgc
    14701 acgatcgact tttcagttta tgccaaccaa tcattgctgg ccaagggtgt aacggaattc
    14761 tcgctgttcg gcggggtcta ttcgacggcc acgttaaccg gattccacta ttcccgcgat
    14821 tggatcgcca atgggttcat ctacaagggg ttggccgagc aactgaccgc gggtctcaat
    14881 tttcaggcga atgatcgcca ccggcaggcg ggcacggaga ttctgtgggg cagcccgatc
    14941 gggcttgccg ggttaaatct tgctgcaaac agctcgcaag cgggagacgg tctggccgtc
    15001 gccatgacct acgagcgttt gttaagtcag aacggcggcg cccacagcca atcgatccgt
    15061 gccgcgatcg aatggcgaag cagccgcttc gatcttgccg agctgacatc cgaccccact
    15121 cgccctcggc tgcgcgccag cgctggctat gtcatcaccc tcggccgcaa cagcttcgtc
    15181 gcggccgacc ttcaatatgc ccgcgattac cgtgcggctc aaagcaacta tggcgggcgg
    15241 ttgagcggcg gcttcgatct tggcgagaga atcgctgcga cggccgagtt cgggatcaac
    15301 cgcggcgagc ccaacaggga cacctacgtc cgcgtgggcc tgcgcatgcg ctttggtggg
    15361 cgggggctgg tccagatcga tgccgacagc aaagggcgca cgcgcgcgaa cttctccgcc
    15421 tctcatggcg gcggtgttgg cgcctggcag ggatctgcga atctcgagca ggatgccaac
    15481 gcggtgtcat tcactgccaa tggcaccctg tcggccaatc gggcggaact gggcgttcag
    15541 gagagcgtca actgggacaa gcggtccggg caattttccg atgctcgcac gacgttgcgt
    15601 gcggccttcg ccatcgccta cgccgacggc agcgtggccc tcggcaggcc gatcaccgag
    15661 gcattcatga ttgccgcgcc gcatcgctcg ctcaacggca aggcggttta tgtcgatccc
    15721 aatgaacaga gcgaggcagc ccgctcgggt caactcggtc cggcgctcga tggccagttg
    15781 ggcgcacaca gtttccgtac gctggtctac caagtacctg acgctccgtc aggctatgat
    15841 ctcggcgcag gaaacgtctc gatttcgccg ccctatcgcg ccggttaccg gctggtcatc
    15901 ggttcggact atcacttgct tgtcattggc cgactgctcg acgggggcgg tgagccgata
    15961 aagctgctcg cgggcagagc cgtcgatctt ggcaatccga agcacccggc tgtcaccttg
    16021 tttacatcac gcgacggcaa gttcggtgcc caagggctgc ggccgggacg ttggcggatt
    16081 gatatgccga ccgatccgcc gacatcctac gaattcgatg taaccgaaac gtctgacggt
    16141 atcgttcgcg tcggagatct gcatcccgta tcgtcgaaaa aagggggacg gtgatgaacc
    16201 ggagattgat ggccgtctgt gcgtgcatag gtgccgccct gggctctgga ggagccgcct
    16261 gcgcagccaa ttgcggcacc attgccgttt cgccatcggc gcccaatttc tcctcgtgga
    16321 atcccctcaa tccggccaat cagcaggtcc cgttcgtggt caccttcgcc cgggcctccg
    16381 cctcgacgaa gagcgtccgg ctgatattcc tcgacgccaa cagcaatgcc attccgacgc
    16441 ggatcggcac gactaccggc ccccgctatc agatcgtcaa taccgacagc ggcgcgatcg
    16501 tctcttttcc ctccggcaca tccgtgacca gccaaaccgt tgcggtaaca cagaccagca
    16561 atggctcggc aaacagcttc acgatcaaca tgagtgccgt agctctcgcc aacagcagcc
    16621 cgaccgaaga tttcatcggc ggcgcggcct ataccgagac gctgaactac gcgatgcaat
    16681 gcttcaaggc caacgggcag gacaacggcg tcgattctgg cgtggtttcc ggtcttaccc
    16741 ttaatctgac gatccccaag atcctgagca ttgtcaccgc cagtccagtt gcgatcgatt
    16801 ttgcaaattt tacctcgaca tcgcagcagg cgcgaatttc ggtgaaaagc accagtactt
    16861 tgaacgtggc ggtatcgacg agctatggta gccggcttgt ccgcaacgga gccgttccac
    16921 cttttccgac caattcaatc attccctatg gcatgaaatt caacggtgtc gcggttgcgc
    16981 caagtcagcc cttggtcaat caggcacgcg ccggcgttac tggcagcagc tatccgctgc
    17041 agttgacgtt gacggatggc attccatccg ggaagctggc cggctcatat tccgacacga
    17101 tcacattgac acttacaccc gggcagtagc ccagcaattc gctgtctgct ttcgggaatt
    17161 tatccagggg ttttgagggg ccgccatggg tcgtttccgt ctgccggcgc cgaggtcgat
    17221 gaatggcggc tatccgctat cgcgcgcgcc aaagccgacg ggccctaacg gccaaatcgc
    17281 cgtcactcct cagcaaagca gccgcgctcg aaaccatatg ctgtaatcgc tcccaatcac
    17341 agcgcatcca acgtccgagg tctggcgtgg ctgctgccga tttgatcgtt ttctggaata
    17401 ggtcatatcc agatcagcgc cttctttgta cgtgacggaa tagatagctc agccttcgcg
    17461 aaacgccgcc aaccatccgg cggaccaacg aaggatcgca gaatgagcaa tcaagttgtc
    17521 ctgatcaccg gcgccctgac tggcatcggg cgtgcggctg ccatcgcgtt tgccaagaag
    17581 ggcgccaagg tcgtcgtttc gggccgtcgc gatgaggtgg gccaggcgct tgccaaggag
    17641 ttgcaggctc tcggatcgga ggccgaattc atcaacgccg acgtccgcaa ggaggatgac
    17701 gtccgcgccc tggtcgacaa ggccgtcgcg cggttcggcc gccttgatgt cgcggtgaac
    17761 aacgcgggca ccgaaggtga ccgcggcatg gtcaccgacc agacggcgga aagctatgcc
    17821 aagacattcg acaccaacgt cctcggcgtg atcctcagca tgaagcatca ggtgcgcgcc
    17881 atgcaggccc aggggagcgg cagcattatc aacatctcgt cgacctatgg gcatgaaggc
    17941 gcggcggggg cctccgtata tgtcgccagc aagcacgccg tcgaaggcct caccaaatcg
    18001 gtggcgctgg aactcgcaaa atcggggatc cgggtgaacg cggtggcgcc tggtgcgacg
    18061 gacaccggca tgctgacacg cttcactgag acaccggaga acaaggcagc cctggtgacg
    18121 accgtgccga tggatcgcct cggccacacc gaggaaatcg ccgagggcat tgtcttcatc
    18181 ggctcggacg aagcttcgta cattaccggc cacgtcctca acatcgacgg tggcaaatcc
    18241 cactgatcct gtctggtgtg aaggtcctct agtccgctcc gcgcgatctc cctggggctc
    18301 cttcacggct ccgggggatc gcctgcattc gaccgcttcg cgtgccccat cgagtggggc
    18361 cgccgacttg gcccggttcc tggacgccga ggcgcgattc cccatcctga aggaggctta
    18421 ccatgtctga tcggccgatg aaggtatcgg ggcccgacca ccccatcgaa atccgaccca
    18481 gcccgtcgcg cgtcgtggtt accatcggcg ggcagatcat cgccgacacc cgtgaggcgc
    18541 tgacgcttag cgaggcggac tatccgtccg tccaatatgt tcctcggcgg gacgtggaca
    18601 tggcggcgct catgcgcagc gatcacgcca gttggtgccc ctacaagggc gaggcatcct
    18661 attacagcat cccggctggt ggagagcgtt cgcacaatgc agtgtggacc tacgagacgc
    18721 cgttcccggc gatggtggcg atcaaagact acattgcctt ctacccggat cgtgtcgacg
    18781 aaatcgctga acgtcctggc gacgcggcga cgctcggggc gcatggaaaa tgaccaacgc
    18841 aaatccgatg cctgtcccgg tgaaagtggc cgtcgtctac cacagcggat acggccacac
    18901 ggtcaaggtc gccgaggccg ttgcccgcgg cgcggccggt gtcgaaggcg ccgaggttga
    18961 gttgatcgag gcgggcgtgg cgccccagcg gtggcaaacg ctggacgatg ccgacgcgat
    19021 catcatgggc gcgccgacct acatgggcag cctgtcggcg cccttcaaag ccttcatgga
    19081 cgacagctcg cacctgcaat acgccgagaa gcgctgggcc aacaaaatcg ccgcgggctt
    19141 caccaacggc gcgtcgcgtg gcggcgacaa gcagaactcg ctcatccagc tcatgacgtt
    19201 tgcggcccag caccagatgc actgggtgaa cctcgggctg aactacggca acaaccggtc
    19261 cgtcaccaac gaggagatcc tcaatcgcga cgcctatacg ctgggaatgg cggcccaggc
    19321 cgacatggac caggggggcg atgtcgctcc accggcatcg gacctccgga cggcggagca
    19381 tctggggcgc cgcgtcgccg aggtcgccca ggagcttgtc gccgggcggg ccgcgttggg
    19441 tcgagccgta cgtccgggca tccccggagg atccgagaac caagacccga agcggcgggg
    19501 gatcgtcgag gcgggcgacg tgatcgccgc ttgagccggc tccacctatt ccattgttct
    19561 agaaaagatg cagacggagg tgttatatat tccataggag cgctgaatgg acagactcgc
    19621 ggccctggag ctcttcgtgc gcgtcgtcga caccggttcg ttctcggcgg tggcgcgcga
    19681 tcaggggatc ggtcagccgg cggtctccaa ggccgtcgtc cagctggagg cctggctggg
    19741 cgtcggcctg ttgttgcggt cgacgcgcag tgtggtccca accgaggcgg gtcgcatctt
    19801 ctatgaacgc gccaggcgga tgatagagga ggcggaggaa gcggtgctgg cggcgcgcgg
    19861 gagcgggtgc ggcctttcgg gaaagcttcg cgtgtcgacc tcggtatgct tcgggcggct
    19921 gcatgtaatt ccccacctgc cggctttctt ggccgaacac ccggacctcc aggtcgagct
    19981 cgtcctcgat gaccggcaag tggacctcgt gaacgaaggc atcgacgtgg cgctgcgcat
    20041 ggggccgatg ccggattcga gcatggtcgc gcgccggatt gcagacggcc gccgtgtcgt
    20101 cgtggcgacg ccggcctatc tcgagcgcca tggcacgccc atgtcccccg tcgagctcgc
    20161 gagccatcag gcgatccttt actcagggcg cgccggctgg acatcctgga ccttccggaa
    20221 ggcagagcag gcggtttcgg tcgagctcca ggggcggttg aagatgactg cggcggaggg
    20281 cctgcgcgag gccgtgaact gcgacttggg gttcacgatc gcctcggagt ggaacttctg
    20341 gcccgagctc aagtccggaa aagtcgtgac gttgctggag gagtgggact tgccatcgac
    20401 tcacctgtcg gccgtctatc cgacggggcg cctcgccagc accaaggcgc gcgccttcgc
    20461 atcgttcgtc gagcgattcc tctcgagttg acttcggttt cgtcacaccc caacgcgcga
    20521 atatccggca tgcggacgaa ggcaaagatt agctcctggc gcagttctga tgcttagaag
    20581 caggactgtc ggcgtccgct ttgcgtcaag agcagtcgac tcacaggtgc aggtcgctcg
    20641 cgtagcctgt caattcgaga tagccgcgcc cgccagaagt ggtgacagcg ccttcccaat
    20701 agatggggct gcctgccgca cgcgcctcca actcctgatc gggaaaaagc gggcggagcg
    20761 gcagtgtgcg gataccgtcg ggcagacgga tacggaggac aggatcgatc ggatagcgcg
    20821 cgccggtgcg cggcgagcgc cagcgctggc ggagttcgaa tgcgacatcg tacgggccga
    20881 ggacagtcag atcgccatcc gccgcccgcc gtgtcccgcc tgcccacaaa gtggagccgt
    20941 ccgcgcggcg gatgcgaaac gccatcagcg ctccgccatc ggtgaagttg agcccggccc
    21001 agtcccatcc ggtcgcgccc ggcggcaggt agctggacga ccattcgcga tcgagccacg
    21061 ctgtcccggt tacaggctcc gcgcggccgc tacgcgtcac cgtgcccgag acgacaagct
    21121 gtgggaggct gtaatagcgg ctcgccgagc gcgggtccgg tcccttccgg ctatagccat
    21181 gatcgccctc ggccagcacc ggctgggttg gttggaagac gagatcaagc gcaaagccgt
    21241 cacccgcgac atgggcgacg aagcgaccgt cgggcaggcg acgcagccgc cagtcgtcga
    21301 tcgcgacatc cgcatcggcg cgcgacgcgg cggcgatgcc cagcccctcg cgcgcgatcc
    21361 tttcgccgtg gagcagatga ccgattgcgg ggtcggagag cgcggcatgg gcgaacagga
    21421 tctggcgcgg cgcaaaggcg ctccggctgt ccgcacccaa tccagtagcg atgcggaaga
    21481 aggtgatctg gaatcctctg tgcgtctcgc cggggccatc cagccagccg gtgagatacc
    21541 accactcggt gcgatagtcc ggatgcgcgc cgtgatcgcg cgggaagacc agcgcgacgc
    21601 cgggtctcac ctcggcaaag cgcgggccgg gcggcgcggc tccgatgagg gtggtcatcg
    21661 ccaacagcac gagcagctgg cgcatcacca atcctcccgt accgcgcgca ccacgtcggc
    21721 cgaaacggcg cgccgcccgg ccagcattgc ggtgagtgcc gacgccacga tgagcgccag
    21781 cgccacgcct gagaggagac cgacgggcaa gtgcgtctcc atcgtccagt tgaacgactg
    21841 cgggttgacg acgcggatca gcacctgcgc aaggccgcag cccagcgcga cgcccgcgat
    21901 cgcgccgatc aggccgagca atgctccctc cattcccagc atggcgataa tctgccgtcg
    21961 ggtcaccccg atgtgacgca gcatgccgaa ttcacgggtg cgggcgatcg tctgcgccga
    22021 ggcggtggcg gcaacgccgg cgagcccgac gaggatcgcg accagctcca gcccataggt
    22081 gatcgcgaag ctgcgatcga agatggcgag cgcgcgtgtt ttgagcgcgc gcgtcgggac
    22141 gatctgcacg ctgtcggcca ccgcgggggg caaggcgccg cgcagcgccg tcatggtcgc
    22201 gccgggatcg gcaccgggca ccagatcgat ggcggcctcg ccccgcgcgg tgtcgccggt
    22261 cagccggaca tagctgcggc cgtcgatcgt aattgcgccc tcctgtcgcg catagtcgcg
    22321 ataaacggcg gtaacggtgg cgggcaggcg gtgggcaccg agcggcagca gcatcacgga
    22381 tccgaccgat agccggtaga gccgcgccgc cggctcggag acgcgcaccg caagcgcgcc
    22441 cgccggcgcc gccacggtgc gcccgatcgc cggtgcggca tcggccgcac cgtctcgcac
    22501 gaggagcgtc actgtcggcc ggttgggcgt gagcgcgagc acatgctggg cgccgaacat
    22561 gatccttccg acgcctggta cggcggcgag gcgggcctgt gtcaccgaat cgaaaggcgt
    22621 gccgccgttc gcgctcagat agagatcagc cgacaggagc tggttcaacc attggtcgac
    22681 cgatccgcgg aagctcgcca ccatcaccgc catcgcgatc atcaggccta cgctggcgac
    22741 gatgcccgac agcgccaccg ccgcctggct cggtgctccc cacagtcggc cgaccgccag
    22801 ccccgtcacg ggcgaggcga aggaatggcg ggcgagcggc gcaagcagcg tccgcgtcag
    22861 ccacggcatc gccacgattc ctgctgccaa cagcagtccg accgcaccat agccgaacaa
    22921 ggcgatgcct cctacggccg gcatcaacgc gaagcctatc ccgatgaagg ccagcagcaa
    22981 cgccggcgcg atccttggcc ggcgacgcgg atcgatcgcg tcgccgagat tcttgagcgc
    23041 caccatgggc gccattcgca ccgcttcata tgccggcagc aggccaccga tcagcgcggc
    23101 ggcgatgcca agcgccgcga a
//
LOCUS       DATGEM010000109         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_996863, whole genome shotgun sequence.
ACCESSION   DATGEM010000109 DATGEM010000000
VERSION     DATGEM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996863"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VKQ27_03480"
     CDS             complement(<1..439)
                     /locus_tag="VKQ27_03480"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617603.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HLY88019.1"
                     /translation="MLTNADLAAKVDTSDEWIVQRTGIRERHIAAKGEFTSDLGTKAA
                     LAALANAGVDAQSIDLIVLGTSTPDNTFPATAVQIQQNLGIHHGVAFDLQAVCSGFVF
                     ALATADNFLRSGSHKRALVIGAETFSRILDWQDRGTCVLFGDGA"
     gene            complement(490..1551)
                     /gene="plsX"
                     /locus_tag="VKQ27_03485"
     CDS             complement(490..1551)
                     /gene="plsX"
                     /locus_tag="VKQ27_03485"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HLY88020.1"
                     /translation="MPSKVRIAVDAMGGDIGPPVVIPGAAISLRRHPETEFLLFGNRA
                     LIEPELAKQPALKAASRIIHTDVAVRMEDKPSQALRRGRRTSSMWLAIDAVKRGEADA
                     AVSAGNTGALMAMARFNLRTIEGVDRPAITAVWPTLRGESIVLDAGASPGADAKHLAT
                     LAVLGSAMARVLFDLERPTVGLLNIGTEEVKGVEEVREAAELLRALNLPHLDFIGFVE
                     GDGIGKGAADVIVTEAFTGNIALKAAEGTARQIGEYLRSAMSRTWRSRIAYLIARDAF
                     TALREKMDPGRGNGGVLLGLNGIVIKSHGGTDAEGFASAVDVGYDMVRYDLLTKINQT
                     LNRDGPAFSLAPVAQGAPS"
     gene            complement(1664..2245)
                     /locus_tag="VKQ27_03490"
     CDS             complement(1664..2245)
                     /locus_tag="VKQ27_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLY88021.1"
                     /translation="MRNDTKSHEVSWTVPVVVADIEEGGLRVAIEADEAERDAIERAI
                     GLRDLSKLSADFELTPLGHEEVQVIGQVNAVVGQTCVVTLDRVDNTVSEPVSLVFAPP
                     AMIAEVHDQDEGDEPPTEDPPEPIVNGRIDLAKLAVEFLILGIDPYPRKPGAVFEPVH
                     TPPAAEDHPFAGLAALKEPKPVSTGQKPPRKRS"
BASE COUNT          428 a          751 c          741 g          401 t
ORIGIN      
        1 cggcgccgtc gccgaacagg acgcaggtgc cgcgatcctg ccaatcgagg atgcgcgaga
       61 acgtctcggc gccgatcacc agcgcgcgct tgtgcgagcc cgaacgcagg aagttgtcgg
      121 cggtcgcgag cgcgaacacg aagccggagc ataccgcctg cagatcgaac gcgacgccgt
      181 ggtggattcc gaggttctgc tgaatctgaa ctgcggtcgc cgggaaggta ttgtctggcg
      241 tcgaggtgcc aagaacgatc aggtcgatgg actgggcatc gacgccggca ttggccagcg
      301 cggcgagcgc ggccttagtg ccaaggtccg aggtgaactc gcctttggcc gcaatgtggc
      361 gctcgcggat cccggtacgc tggacgatcc attcgtccga ggtatcgacc ttcgcggcga
      421 gatcggcatt ggtcagcact tgctccggaa gataggagcc gcagccaagg acgatcgaac
      481 gggtaacggt cacgagggag ccccttgcgc tacaggcgcg aggctgaatg ccgggccgtc
      541 gcgattgaga gtctggttga tcttggtgag aagatcgtaa cggaccatgt catagccaac
      601 atcgactgcc gaggcaaagc cttcggcatc ggttccgccg tggctcttga tgacgatgcc
      661 gttcagcccg agcaggacac cgccgttgcc cctgcccgga tccatttttt cgcgcaacgc
      721 ggtgaaggca tcgcgcgcga tcaagtaggc gatccgcgag cgccacgttc gtgacatggc
      781 gctgcgcaga tattcaccga tctgccgggc ggtcccttcg gccgccttca gtgcaatgtt
      841 gccggtaaac gcttcggtga cgatgacgtc ggcggcgccc ttgccgatgc cgtcgccctc
      901 gacgaagccg atgaaatcga gatgcggcag gttcaacgcg cgcaacaact cggcggcttc
      961 ccgcacctcc tcgacgccct tgacctcctc ggtcccgata ttgagcagcc ccaccgtcgg
     1021 ccgctcgaga tcgaacagca cccgcgccat ggcactgccg agcaccgcaa gcgtggccag
     1081 gtgcttcgca tcggctcccg gcgaagcgcc ggcatcgagc acgatcgact cgccacgcag
     1141 ggtcggccaa accgcggtaa tcgccggacg atcgacgccc tcgatcgtcc gcaagttgaa
     1201 gcgcgccatc gccatcagcg cgccggtatt gccggccgag accgcggcat cggcctcgcc
     1261 gcgcttcacc gcatcaatcg cgagccacat cgaggaggtc ctgcggccgc gacgcagcgc
     1321 ctggctcggc ttgtcctcca tgcgcacggc aacgtcggta tggatgatgc gggaggccgc
     1381 cttcagggca ggctgcttgg cgagctccgg ctcgatcaac gcgcggttgc cgaacagcag
     1441 gaactccgtc tccgggtgcc gtcgcaacga gatcgcggcg ccggggatga cgaccggcgg
     1501 accgatgtcg cctcccatcg catcgaccgc aattcgaacc ttgctgggca tgtaaggtac
     1561 aggaacccct gcgaaaccgt tggttagaag gccaacctgc cgtcaaattg ggcggcggac
     1621 aatagcgtgt gcgccgcctg acacaactgt tgacaggcca cgctcaggac cgcttgcgcg
     1681 ggggtttttg gcccgttgag acgggctttg gctctttcag cgccgcgagc ccggcaaacg
     1741 ggtgatcctc cgccgccggc ggggtatgga ccggctcgaa taccgcgccc ggtttgcgcg
     1801 gataaggatc gatcccgaga atcaggaact cgacggcgag ctttgccaga tcgatccggc
     1861 cattgacgat cggctccggc gggtcctctg tgggcggctc atcgccctcg tcctgatcat
     1921 gcacttcggc aatcatggct ggcggagcaa acaccaggct gaccggctcg ctcacggtgt
     1981 tgtcaacccg atcgagggtc acgacacagg tctggccgac caccgcattg acctgaccga
     2041 tcacctgaac ctcttcgtgt ccgagcgggg tcagttcaaa atcggccgac agtttggaca
     2101 agtcacgcag gccgatagcg cgttcgattg cgtcacgctc ggcttcatcg gcttcgatgg
     2161 ccacgcgcaa cccaccctcc tcaatgtcgg caacgacaac gggtaccgtc caggacacct
     2221 cgtgcgattt ggtatcgttg cgcatcacgg actcttattc gggactcgga aagcgccacg
     2281 caccggacaa cagtgcgggg gcatcaatgg ttgtgagacc t
//
LOCUS       DATGEM010000110         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1106261, whole genome shotgun sequence.
ACCESSION   DATGEM010000110 DATGEM010000000
VERSION     DATGEM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106261"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1780
                     /locus_tag="VKQ27_03495"
     CDS             275..1780
                     /locus_tag="VKQ27_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HLY88022.1"
                     /translation="MRGSCCLGLCARGPLVRVELDTGREMVYQQVQPTQATEIAASLA
                     GHAAAPVNQLDLQAPFFSRQVRVVTANAGRIDPESIDDYLAVGGYEALSRAVTSTSPQ
                     DVVAEVSRSGLRGRGGAGFPTGVKWGLVARVAGDDKYVICNGDEGDPGAFMDRSVMEG
                     DPHRVLEGMAIGAYAVGASQGYIYVRGEYPLAIRRLQRAIRQAEHQGVLGTRICDTPF
                     HFRVDIRIGGGAFVCGEETALIASIEGGRGQPHARPPYPAQAGVWGKPTLINNVETFA
                     NIAPIVMQGGAWYAGYGTEKSRGTKVFALAGKINNTGLVEVPFGITVRELIEDIGGGI
                     AGGRPFKAIQTGGPSGGCVPASMLDLPIDYDSLASVGTIVGSGGLLVMDDSACMVDVA
                     RFYMDFCMHESCGKCIPCRTGTAEAVRILDRLCSGLGTPDDLERLELLLPYMQDASLC
                     GLGQTAPNPVVSTLRYFRDEYLAHIQERVCPAGVCRMTGAPTPQARAEARA"
     gene            1819..2553
                     /gene="hoxU"
                     /locus_tag="VKQ27_03500"
     CDS             1819..2553
                     /gene="hoxU"
                     /locus_tag="VKQ27_03500"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HLY88023.1"
                     /translation="MTLTLDGELVSARQGDKLLHVIQEHGGHVPTLCEFDGLSSVAAC
                     RLCLVEIAGRPRPAAACVTAAEEGLDVRTDSERIRRYRRMTLELLLAERNHICSVCVM
                     NGSCELQDRAAEAGIDHVRFDYAWQTNLRVDASHDLFVLDENRCVLCTRCVRICDELE
                     GVHTWDLSGRGIDARVITDMAIPWGESITCTSCGKCVQVCPTGALFRKGVTIAEMTKD
                     KEKLNFLSDARHERIWDLQRIVDEKP"
     gene            2550..3095
                     /locus_tag="VKQ27_03505"
     CDS             2550..3095
                     /locus_tag="VKQ27_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HLY88024.1"
                     /translation="MSARKPRIATVWLGGCSGCHMSFLDLDERLLELAQLADVVFTPV
                     ADIKVFPADVDVTLVEGAVANEDHLTMLQTVRQRTRVLVSLGDCAITGNVSALRNVWP
                     VDVVLRNSYIERSTVSGLPDEAGVVPRLTARVAPLHHVVTVDEFIPGCPPDAERIWRA
                     LLALLAGEHVRPEDAPAEKFG"
     gene            3101..>3608
                     /locus_tag="VKQ27_03510"
     CDS             3101..>3608
                     /locus_tag="VKQ27_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HLY88025.1"
                     /translation="MAERRILIDPVTRIEGHAKISILLDDSGIVTDARFHVTEFRGFE
                     TFCQGRLLWEMPGLCARVCGICPISHLLASAKAGDMILGVRIPVAAEKLRRLANLAQI
                     VQSHALSFFHLSSPDLLLGFDAPPESRNIFALASSHPDITRAGIRLRGFGQEVLAELT
                     GKRIHASWA"
BASE COUNT          619 a         1134 c         1183 g          672 t
ORIGIN      
        1 gtcaactgtc ggtggccatc gcgcgatgtt tcgggagctg tgcactggca ccggtgctgg
       61 tgatcgacgg tcaggtgatg ggaaatagct ctccggatgc ggcaatcgaa cgcgtccgca
      121 cggcgctgtc cacccctacg cgacgcgaaa acgaccttca gccggtgcca gcgtgacagc
      181 acgtgtattc acctgcacgg gcaccgcgtg tctcagtctg gagtctgagg cagtcaagaa
      241 tcagctccag caatctcttg gaaaggacat cgaggtgcgc ggcagctgtt gcctcgggct
      301 gtgcgcgcgc ggtccgctgg tgcgcgtcga gctggacact gggcgcgaaa tggtgtacca
      361 gcaggtccag ccaactcagg cgaccgaaat cgcggcatcc ctcgccggcc atgcagcagc
      421 ccctgttaac caactggatc ttcaggcgcc gttcttcagt cgccaggtca gggtcgtcac
      481 ggccaacgcc ggtcggatcg atcccgagag tatcgacgat tatctggccg tcggcgggta
      541 tgaagcgttg agcagggctg taaccagtac gagcccgcag gacgtggtag ccgaagtctc
      601 gcgcagcggc ttgcgcggcc gcggtggcgc cgggttccca actggcgtga agtgggggtt
      661 ggtggctcgc gttgccggcg atgacaagta cgtcatctgc aacggcgacg agggggatcc
      721 aggtgcgttc atggaccgct cggtcatgga gggtgatcca caccgcgtcc tggagggcat
      781 ggccatcggc gcctacgcgg ttggcgccag tcagggctac atttacgtgc gcggcgaata
      841 tccgctggcg attcggcggc tgcagcgcgc tattcgccag gcggagcacc agggcgtgct
      901 tggcacacgc atctgcgata cgccattcca ctttcgcgtg gacatccgca tcggtggcgg
      961 cgcctttgtc tgcggggagg agaccgcttt gattgcgtcg atcgaaggcg gtcgcgggca
     1021 gccgcatgcc cgtccgccct atccggcgca ggcgggcgtt tggggcaagc cgaccctgat
     1081 caataacgtc gagaccttcg ccaacatcgc gccgatcgtg atgcagggcg gtgcgtggta
     1141 cgccggctat ggcacggaaa aaagccgagg caccaaagtc tttgcgctgg ctggcaagat
     1201 caacaacacc ggcctcgtcg aagtcccttt cggcatcacc gttcgtgagc tgatcgagga
     1261 catcggtggt gggatcgccg gtggccggcc attcaaagca atccagactg gcggaccgtc
     1321 cggcggctgt gtgcccgcgt ccatgctgga cctgccgatc gattacgact cgctcgcctc
     1381 ggtcggcacg atcgtcggct caggcggctt gctggtcatg gacgatagcg cgtgcatggt
     1441 ggacgtggcg cgcttctaca tggacttctg catgcacgaa tcctgcggca agtgcattcc
     1501 atgtcgcacc ggaaccgccg aagcggtgcg catcctggac cgcttgtgca gtggactcgg
     1561 cacacccgac gacctggagc gattggagct actgttgcca tacatgcaag acgccagcct
     1621 gtgtgggttg ggtcagactg caccaaaccc ggtcgtttca accttgcgct atttccgcga
     1681 cgaatatctc gcgcacatcc aggagcgtgt gtgcccagcc ggtgtgtgtc gcatgactgg
     1741 cgcgcccacg ccacaggcac gcgcggaggc gcgcgcatga cgcacgcacc ggatccagtg
     1801 cctgctacgc cgccttcggt gacgctcacg ctggatggtg agctcgtgag cgcacgtcag
     1861 ggtgacaaac tgctccatgt catccaggaa catggcggtc acgtgccgac gctgtgcgag
     1921 ttcgacgggc tgagcagcgt ggccgcctgc cgcctgtgtc tggtggagat cgccggacgg
     1981 ccccgccccg cggcggcatg cgtcaccgcg gctgaggaag gcctggacgt tcggacggat
     2041 tcggagcgca tccgccgcta ccgtcgcatg accctggagc tgctgctggc ggagcgcaac
     2101 catatctgct ccgtgtgtgt gatgaatggc agttgcgagc tgcaggaccg cgccgccgaa
     2161 gccggcatcg accatgtccg cttcgactac gcctggcaaa ccaatctgcg cgtggatgcg
     2221 tcgcatgacc tgttcgtgct cgacgagaat cgctgcgttc tctgcacacg ctgcgtacgc
     2281 atttgcgacg agctcgaagg ggtgcacacg tgggatctgt ccggccgcgg catcgacgcg
     2341 cgcgtgatca ccgacatggc tatcccctgg ggagaatcga tcacctgtac atcatgcggt
     2401 aagtgtgtgc aggtctgccc cacaggggca ttgttccgca agggcgtgac catcgccgag
     2461 atgaccaagg acaaagagaa gctgaacttc ttgagcgatg cgcgtcatga gcgcatctgg
     2521 gaccttcagc gcatcgtgga tgagaagccg tgagcgctcg caaaccgcga attgcgacgg
     2581 tatggctggg cgggtgctcg gggtgccaca tgtcgttcct ggacctggac gaacgcttgc
     2641 tcgagctggc ccagttggca gatgtcgtgt tcactccagt tgctgatatc aaggtctttc
     2701 cggcggacgt ggatgttacg ctggtggaag gcgcggttgc caacgaggat cacctgacga
     2761 tgcttcagac agtccgccag cgcacgcgcg tgctcgtctc actcggcgat tgcgcaatca
     2821 ctggcaacgt cagcgccttg cgcaatgtgt ggcccgtcga cgtggtgctg cgcaactcgt
     2881 acatcgaacg ctcaacagtt tcaggactcc ccgatgaggc tggcgtagtc ccgaggctaa
     2941 ccgcgcgcgt ggcgccactc catcacgtcg tcaccgtcga cgagttcatc cccggctgcc
     3001 cgcccgacgc cgagcgcatc tggcgggcct tgctggccct gctcgccggc gagcacgtgc
     3061 ggcctgaaga tgcgccagcg gagaagtttg gctaacgctg atggctgagc gccgcatcct
     3121 gatcgacccg gtcacgcgca tcgaaggcca cgccaagatc agcatcctcc tggatgactc
     3181 gggaatagtc accgacgccc gcttccacgt aaccgagttc cgcggctttg agacgttctg
     3241 ccaggggcgc ctgctgtggg agatgccggg cctgtgcgcg cgcgtctgcg gcatctgtcc
     3301 gatcagccac ctgctggcct ccgcaaaggc cggcgacatg atcctgggag tccgcattcc
     3361 ggtggccgcg gaaaagcttc gccggttggc gaacctggcc cagatcgttc agtcgcatgc
     3421 gctatcgttc ttccacctct cgtcgccgga cctgctgctg ggcttcgatg ctccgccaga
     3481 gtcgcgcaac atcttcgcgc tcgccagctc acacccagac atcacccgcg ccggcatccg
     3541 cctgcgcggc ttcggccagg aagtgctggc ggagctgacc ggcaagcgca tccatgcttc
     3601 atgggcgg
//
LOCUS       DATGEM010000111         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1223576, whole genome shotgun sequence.
ACCESSION   DATGEM010000111 DATGEM010000000
VERSION     DATGEM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223576"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="flgG"
                     /locus_tag="VKQ27_03515"
     CDS             <1..634
                     /gene="flgG"
                     /locus_tag="VKQ27_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HLY88026.1"
                     /translation="GSNSSDSGSIVPSGAQVGLGVKTAAIYRINEQGNLQQTSNTLDM
                     AIQGNGYFQVTLPSGDTAYTRDGTFGLAADGTIVTADGYVVQPGVQIPSNATGVTVNQ
                     SGQVQVTLQGQTAPQTAGQLQIATFPNEAGLDAQGDNLFLQTAASGQPVTGNPASPGF
                     GSVMQGFIETSNVNVVTEITNLITAQRAYEMNSRVITTSDEMLSTLTNLR"
     gene            639..1613
                     /gene="flgA"
                     /locus_tag="VKQ27_03520"
     CDS             639..1613
                     /gene="flgA"
                     /locus_tag="VKQ27_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HLY88027.1"
                     /translation="MRNSPYLRGSLAIPALLALLGMPMQPDAATLRTMTTLHASTVRL
                     SDLFDNAGEKADVVLGPGPAAGARIVVEAPQLAAIARQFRVDWRPVSSADRAVLERSG
                     KPLKRDDVLTAVRSALVARGVSDDCDVELTEFMGPQVPFESQPLPVVSDLDFDASAGR
                     FAAMLSIAGAGMEPIHLRVSGKVDDTMELPVATSRLPAGAVLSQDDVRMARVRVSYVH
                     GEVVRRLSDVIGLQLKHQVAPGLPLVRSDLSRPSLVLKGARVLMQLDIGGISLTAQGQ
                     AMEAGAVGERIRVLNPVSRAVVEGEVIGPDRIRVAPNSMPRPTEVSMP"
     gene            1610..2347
                     /gene="flgH"
                     /locus_tag="VKQ27_03525"
     CDS             1610..2347
                     /gene="flgH"
                     /locus_tag="VKQ27_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HLY88028.1"
                     /translation="MNTSLRLLAPTLACLLLAGCGSLQRLSEVGRPPAMTPTSDPTKD
                     PNWRPVSMPMPYREPVPNEANALWRPGSRAFFKDQRAAQVGDIITILVSMNDTANVKD
                     QTTNSRTSGATGSLQNFFGMETLLPKTISDPSKLLNVGSTNTQGGSGQIQRSETVTLR
                     LAGVVTQVLPNGNLVVAARQEFRVNRELRELQVTGVVRPQDIASDNTVQHDRLAEARI
                     TYGGRGELTETQHTPWGQQLLNILSPF"
     gene            2566..3738
                     /locus_tag="VKQ27_03530"
     CDS             2566..3738
                     /locus_tag="VKQ27_03530"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HLY88029.1"
                     /translation="MINFDAVMQITKRPSIVFVAGQGSWLTDSDGRRYLDFIQGWAVN
                     TLGHSPPAIVQAMAHQAQRLLNCSPAFYNDQMVKLCDLLARHSGLHQAYLANSGAEAN
                     EGAIKLARKWGAKYRDGAYEIITMDHGFHGRTLATMAASGKPQWEQLYAPKVTGFVKV
                     KLNDLAAVEAAITPRTVGVMLEPIQGEAGVFEATDAFMQGLRTLTRERGLLLILDEIQ
                     TGIGRTGKLFGFEHPGTQPDIMTLAKGLGGGVPLAALVAHRDVCCFESGDQGGTFGGN
                     AIMASVGCAVLEQVTQPGFLARVTQAGEHLQGALQMLSRKHGGGEVRGRGLLQALNLG
                     RDISMQVADAALERGVLLNAPRPDSLRFMPSLTVTDSEIDQMIEILDDVLDQMATA"
BASE COUNT          698 a         1143 c         1274 g          689 t
ORIGIN      
        1 cgggtccaac agttccgaca gcggcagcat tgtgccatcc ggtgcgcagg tcggtctcgg
       61 cgtgaagacc gcggcaatct accggatcaa cgagcagggc aacctgcagc agacgtcaaa
      121 cacgctcgac atggcgattc aaggcaacgg ctatttccag gtaaccctgc cgtcgggcga
      181 caccgcgtat acacgcgacg gtacattcgg gctggcggcg gacggcacta tcgtcactgc
      241 cgatggctat gtggtgcagc caggcgtgca gatcccatcg aatgccaccg gggtcaccgt
      301 aaaccagtcg ggtcaggtgc aggtcacgct gcaggggcag acagcaccgc agaccgctgg
      361 gcagttgcag atcgcgacgt ttcccaacga ggcgggactg gatgcacagg gtgacaatct
      421 gttcctgcag accgcagctt ccggccagcc ggtcaccggc aatccggcat cgccgggctt
      481 tggttcggtc atgcagggtt tcatcgagac atccaacgtc aacgtggtga ccgaaatcac
      541 caacctcatc acggcgcagc gggcctacga aatgaacagc cgggtcatca cgaccagcga
      601 tgagatgctg tcaacgctga ccaacctgcg gtgatgccat gcgcaactca ccatatctga
      661 gaggctccct tgcgatcccg gcgcttctcg ctctgctcgg catgccgatg cagcctgatg
      721 cagccacgct gcgcacgatg accacgctgc atgcatcgac cgtgcgcctg tcggatctgt
      781 tcgacaacgc cggagagaag gcggacgttg tcctggggcc gggccccgca gccggtgcgc
      841 gcattgtggt cgaggcaccg cagcttgctg cgatcgcgcg gcagttccgt gtggattggc
      901 ggccagtgtc ctccgccgat cgagccgtac tggaacgctc aggcaagccg ctgaagcgcg
      961 atgatgtgct cactgcggtc agaagcgcat tggtggcccg tggagtctcc gacgattgcg
     1021 atgtcgagct caccgaattc atggggccgc aggttccgtt cgagtcacag ccgctgcccg
     1081 tggtgtcgga tctcgatttc gacgccagtg caggccggtt cgcggccatg ctgtcgatcg
     1141 ccggtgccgg catggagccg attcacctgc gagtgtccgg caaggtggac gacacgatgg
     1201 aactaccggt cgcgaccagc cgcctgccag cgggtgctgt gctgagccag gacgatgtgc
     1261 gcatggcgcg cgtccgcgtc tcgtatgtgc atggtgaggt ggtgcgccgc ctgtccgacg
     1321 tgatcggttt acagctgaaa catcaggttg cgcccggcct gccgcttgtc aggagcgatc
     1381 tgtcgcggcc ttccctggtg ctaaagggag cgcgcgtgct gatgcagctc gatattggcg
     1441 gcatctcgct cacggcgcag ggccaggcga tggaggctgg ggcagtcggc gaacggatcc
     1501 gcgtgctcaa cccagtgtcg cgcgccgtcg tcgaagggga agtgataggc ccggatcgga
     1561 ttcgtgtcgc cccgaacagc atgccgcggc caaccgaggt gagcatgcca tgaatacgag
     1621 cttgcgtctt cttgcgccca cactggcgtg tctgctgttg gcgggctgcg gctcgttgca
     1681 gcgcttgtcg gaggtgggcc ggcctcctgc aatgacgcca acgtcggatc ctaccaagga
     1741 cccgaactgg cggccggtga gcatgccgat gccttatcgg gaaccagtac caaatgaagc
     1801 gaacgcgctt tggcgtcctg gttcccgtgc cttcttcaag gaccagcgcg cggcgcaggt
     1861 tggcgatatc atcaccattc tggtcagcat gaatgacacc gccaatgtga aggaccagac
     1921 gacgaacagc cgcaccagcg gcgctacagg cagcctgcag aatttcttcg gcatggaaac
     1981 cctgttaccg aaaacgatct cggatccgtc gaaactgctg aatgtgggca gcaccaacac
     2041 ccagggcggc agcggacaga tccagcgtag tgaaacggtg acgttgcggc tcgctggcgt
     2101 ggtgacgcag gtattgccga acggtaatct tgtggtcgcc gcgcgccagg aattccgcgt
     2161 gaaccgggag ctgcgcgagc tgcaggtaac gggtgtggtc aggccgcagg acatcgcatc
     2221 cgacaacacc gtgcagcacg atcgtctggc ggaggcgcgg atcacctatg gcggccgcgg
     2281 cgaactcacc gagacgcagc acacgccgtg gggccagcag ttgctgaaca tcctatcgcc
     2341 attctgacgt tgactattgg acggcaaccg tgcacagggc acccatgcat tgcgcttcct
     2401 gcatcgtcat ccgccgacgc cgccgtcgtt tgacgggggt ggagggtttg cgcgcgcggt
     2461 agtcagcact gcatagcaac cacgaaagcc cccgtcggca gcgatggggg ctttcgtgtt
     2521 tcagggtctc tgtcgctccg tccccaaccc aggcaggagg acagcatgat caacttcgat
     2581 gccgtgatgc agatcaccaa gcggccatcc atcgtattcg ttgccggaca aggttcatgg
     2641 ctgaccgaca gtgacgggcg ccgctatctg gatttcatcc agggttgggc ggtgaacaca
     2701 ttgggccact cgccgccggc catcgtgcag gcgatggcgc atcaggcaca gcgtctgctc
     2761 aattgcagtc cggccttcta caacgaccag atggtgaagc tgtgcgatct gctggcgcgg
     2821 catagcggct tgcatcaggc gtatctggca aacagcggcg ccgaggcgaa cgagggcgcg
     2881 atcaagctgg cgcgcaaatg gggcgcgaaa taccgtgacg gcgcttacga gatcatcacc
     2941 atggatcatg ggtttcatgg ccggacgctt gccaccatgg cggcatccgg caagccgcaa
     3001 tgggagcagc tttacgcgcc gaaggttacc gggttcgtga aggtgaagtt gaacgatctg
     3061 gctgcggtgg aagccgcgat cacaccacgg accgtcggcg tgatgcttga gccgatccag
     3121 ggcgaggccg gcgtgttcga ggcgaccgac gcgttcatgc aggggctgcg cacgctgacg
     3181 cgcgagcgtg gcctgctgtt gatcctggat gagatccaga ccggcatcgg ccgcactggt
     3241 aaactgttcg gcttcgagca tcccggcaca cagccggaca tcatgacgct ggccaagggg
     3301 ctgggcggcg gtgtgccgct tgccgcgctg gtcgcgcatc gcgatgtctg ctgcttcgag
     3361 tctggcgatc agggcggcac gttcggtggc aatgccatca tggcgtcggt tggctgcgcg
     3421 gtgctggagc aggtgaccca acctgggttc ctggcgcgtg tgacgcaggc gggtgaacat
     3481 cttcagggag ctctgcagat gctctcgcgc aagcatggtg gcggggaggt gcggggcagg
     3541 ggactgctgc aggcgctcaa tctgggccgc gatatttcga tgcaggtggc ggatgcagcg
     3601 ctggagcgtg gcgttctgct gaatgcgccg cggcctgatt cgctgcgctt catgccatcg
     3661 ctgaccgtca ctgattcgga gatcgaccag atgatcgaga tcctcgacga tgtgctggat
     3721 cagatggcca ccgcgtgacg tgcggctccg gcacgtagat acgtgtcgaa cctcgcggcg
     3781 gccgtccgca ggaaaggccg gccg
//
LOCUS       DATGEM010000112         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1105664, whole genome shotgun sequence.
ACCESSION   DATGEM010000112 DATGEM010000000
VERSION     DATGEM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105664"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VKQ27_03535"
     CDS             complement(<1..300)
                     /locus_tag="VKQ27_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88030.1"
                     /translation="MLQSRDEIRKDKTVDAFTDAILQRDQPRTAELFFNLVRDGRSMG
                     DALSLVTEAEAPFVQVPNHVDVRDGQITLINNDHTILGLRTSADLTSYLPQQYRML"
     gene            complement(462..1202)
                     /locus_tag="VKQ27_03540"
     CDS             complement(462..1202)
                     /locus_tag="VKQ27_03540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLY88031.1"
                     /translation="MIEQTLDITTKAGAMETFICHPDRGGPHPAVMVLMDAPGIREEL
                     RDMARRLATVGYYVLLPNLYYRAGRDTIYGPDVLQHGSAEHQRMRAVRTKMTIPPVMD
                     DVASMLAFIDSQPAAKAGPVGAHGYCMSGPYALAAAARYPDRVTAAASFYGTWLVSDA
                     EESPHLNLGKIKGEVYISCAEHDELAPLPMVEELRGLLARSGAAGELELHPGVHHGFA
                     FPQRWCYDKPAAERHWERLIALYRRRLG"
     gene            complement(1199..>2925)
                     /locus_tag="VKQ27_03545"
     CDS             complement(1199..>2925)
                     /locus_tag="VKQ27_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY88032.1"
                     /translation="TDLWSNMSAEAEVFAIHMTYGADADYKTSGREPTDYVNSPLIVM
                     WGWSPGDGTFGTGTLQYLKAAKQRGTRFVCVDPRLTMTSRQLADEHIFIRPGTDAAAL
                     IAMAYVIVSEGLHDQAYCNRYVHGLAEYRAYLSGENDGVAKTPEWAAPITGIPADTLR
                     RLAIEFASTKPAALQTGYAGGRTAYGEQFHRAAYALAAITGNVGIVGGSSGTSNGATG
                     RSGIRSLPIGRNPTGARVATPLLADLLARGKAGGYPADIKLIYAAGGDLFNQCPNVSR
                     MVSSMDGVEFFVAQDHFLTPTARYADLVLPATTFWERNDVHTPWAGAGHYAIFMRQAI
                     QPMYECRNDIDIFSDLARRVGVENYNDKSESEWLRELTAGAIDDFDEFAHQGVARFPA
                     PEDAVAFARQIRDPDRQKFSTPSGKIEIYSEVLAARPDPYGLGVIPPVPTWIEPEQAP
                     AAYPLALCTPKSRARTHSIHGNQAHLARVDPDNVWLHPTNAAERAITDGQHVRVFNER
                     GATVLPVKVTDRIAPGVVSIKEGAWFIPDHTGVDTQGCANVLTVDRSAPCGATTYNSN
                     WVQVEAVR"
BASE COUNT          499 a          913 c          969 g          544 t
ORIGIN      
        1 gagcatgcga tattgttgcg gcagatacga tgtgaggtcg gctgaggtac gcaggccgag
       61 gatggtgtgg tcgttgttga tcagcgtgat ctggccgtcg cgcacgtcca cgtgattggg
      121 gacctggacg aacggcgctt ccgcttcagt gaccaggctg agcgcgtcgc ccatggagcg
      181 gccatcgcgt accaggttga aaaagagctc agcggtgcgg ggttgatcgc gttgcaggat
      241 ggcgtcggtg aatgcgtcga cagtcttgtc cttacgtatt tcgtcacgac tttgcagcat
      301 ggccgttcct tcgcttcttc tggcggctac tggccggtag cctagcgccg cgaggcgaag
      361 gtcgtaaagc tacgcgctgt cattgcgagg agcggcagcg acggagcaac ccccgccgag
      421 attgctccgt cgctgccgct cctcgcaatg acagggaggg attatccgag acggcgccgg
      481 taaagcgcga tcagccgctc ccaatgtcgt tccgcggctg gcttgtcgta gcaccagcgc
      541 tgcggaaagg cgaagccgtg gtgcacgcct ggatgcagct ccagttcgcc tgctgcgcct
      601 gagcgcgcga ggaggccgcg cagctcttcg accatcggca gtggcgcaag ctcatcatgt
      661 tcggcgcagg agatgtagac ctcgcctttg atcttgccga ggttgaggtg cgggctttct
      721 tccgcgtcgc tcaccagcca ggtgccgtag aacgatgccg ccgccgtcac gcgatccgga
      781 tagcgtgccg cggccgccag ggcgtatggg ccgctcatgc agtagccgtg cgcaccgacc
      841 gggccggctt tcgcggcagg ctgcgagtcg atgaacgcca gcatcgacgc gacatcgtcc
      901 attaccggcg ggatcgtcat cttggtgcgc accgcgcgca tgcgctgatg ctcggcgctg
      961 ccgtgctgca acacgtccgg gccgtatatc gtgtcgcgac cggcgcgata gtacaggttg
     1021 ggaagcagca cgtagtagcc aacggtggcg aggcggcgcg ccatatcgcg taattcctcg
     1081 cggatgccgg gcgcgtccat cagcaccatc acggcaggat gcggaccacc acgatcggga
     1141 tggcagatga atgtctccat cgcgcctgcc ttggtggtga tgtcaagagt ttgctcgatc
     1201 atcggacggc ctccacttgc acccagttgc tgttataggt tgtcgcgccg cacggtgccg
     1261 accgatcaac ggtgagcacg ttggcgcagc cctgcgtatc gacgcctgta tggtcgggga
     1321 tgaaccacgc cccctccttg atcgatacca cgccgggcgc aatgcgatcg gtcaccttga
     1381 ccgggagcac cgtggcgccc cgttcgttga acacacggac atgctgcccg tcggtgatag
     1441 cacgctcggc cgcattggtg ggatgcaacc acacattgtc cggatcgacg cgcgccagat
     1501 gcgcctggtt gccgtggatc gaatgcgtgc gcgcccgcga ttttggcgtg cagagcgcga
     1561 gcggataggc ggcgggagcc tgctccggct cgatccaggt gggaacggga gggatgacgc
     1621 caaggccgta gggatcgggt ctggctgcga gcacctccga atagatctcg atcttgccgg
     1681 atggcgtgga gaatttttgg cgatcaggat cgcggatctg gcgcgcgaag gcgaccgcat
     1741 cctcgggggc gggaaagcgc gcgacaccct ggtgcgcgaa ctcgtcgaag tcatcgatag
     1801 cgccagccgt cagctcccgg agccattcgg attcgctctt gtcgttgtaa ttctcgacgc
     1861 cgacgcgacg cgccagatcg ctgaagatgt cgatgtcgtt gcggcactcg tacatgggct
     1921 ggatcgcctg gcgcatgaag atggcgtagt ggccggcacc cgcccagggt gtgtgcacgt
     1981 cgttcctctc ccagaaggtg gtcgccggca gcacgaggtc ggcatagcgt gcggtcggtg
     2041 tcaggaagtg gtcctgcgcg acgaagaact ccacgccatc catcgaggaa accatcctgc
     2101 tgacgttcgg gcactgattg aagagatcgc caccggccgc gtagatcagc ttgatgtccg
     2161 ccggatagcc gcccgccttc ccgcgcgcca gcagatccgc aagcagcggc gtcgcgacac
     2221 gcgcgcccgt cgggttacgc ccgatcggca ggctgcgaat gccggacctt ccagtcgcgc
     2281 cgttgctggt gcccgaactg ccgcccacga tgccgacatt gccggtgatc gctgccaacg
     2341 cgtatgccgc gcgatggaac tgctcgccat acgctgtgcg gccgccggcg tagccggtct
     2401 gcaatgcggc cggctttgtg ctggcgaact cgatcgccaa ccggcgcagc gtatccgcgg
     2461 gaatacctgt gatcggcgca gcccattccg gcgtcttggc gacgccatca ttctcacccg
     2521 acagatacgc gcggtactcg gcgagcccat gcacatagcg gttgcaatac gcctgatcgt
     2581 gcagcccctc cgacacgatg acataggcca tcgcgatcag cgcggccgca tcggtgccgg
     2641 gacggataaa gatgtgctcg tcggcaagtt gccggctggt catcgtcagc ctcggatcga
     2701 cgcagacgaa tcgcgtgccg cgctgcttcg ccgccttcag atattgcaag gtgccggtgc
     2761 cgaacgtgcc gtcgcccggg ctccagcccc acatcacgat cagcggcgaa ttcacataat
     2821 ctgtcggttc gcggcccgag gtcttgtagt cggcatccgc cccgtaggtc atgtggatgg
     2881 caaacacttc ggcctcggcc gacatgttcg accagagatc agtgc
//
LOCUS       DATGEM010000113         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1681217, whole genome shotgun sequence.
ACCESSION   DATGEM010000113 DATGEM010000000
VERSION     DATGEM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681217"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..894)
                     /locus_tag="VKQ27_03550"
     CDS             complement(184..894)
                     /locus_tag="VKQ27_03550"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88033.1"
                     /translation="MNHHFGVLIPSTNTTVEIEYSHLLPPSLQFHVGRLLTSGTGPFS
                     PSKNEDIDYQSRLLATARVEVISLAQTSASLFADDYDAVTTRRITAAANVPAVTSAQA
                     VGQAIRALGTTRIAIVSPYSQQVIERARHYYESKFALSVVALEGFAATDSYMIGKLGP
                     ENARDAFARINRPEIDCFVVPGGNFPTMSFVPAWEQEFGKPVITTNGAALWSMMHIMG
                     ARDPLPTLGRLLAELPAA"
     gene            complement(891..1466)
                     /locus_tag="VKQ27_03555"
     CDS             complement(891..1466)
                     /locus_tag="VKQ27_03555"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HLY88034.1"
                     /translation="MGSRPSPADTLPDLLRHGLRVVFVGINPSLYSVAQGHYFARRGN
                     RFWPCFSRSTLSAEARHALAVATLLPEHDQSLPGHGFGFTDLCKRATARAGDLSPNEL
                     AEGVSVLVAKLVDCRPGIACFHGMTGYRHIQRVLDPASPDAILGLQPLYIGATRCYVV
                     PNPSGANAHFTPADQTSWYDRLAQETTGGTT"
     gene            1511..2644
                     /locus_tag="VKQ27_03560"
     CDS             1511..2644
                     /locus_tag="VKQ27_03560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88035.1"
                     /translation="MKVGVSLSILAQAGRSDVAVYKEHLELGDLAEPLGFDSIFALEH
                     HFTGYSMSPAPVQLLSYFAGRTKRVQLGTAVIVLPWHDPIRVAESIALLDVLCGGRCL
                     FGFGRGAASVEYAGFRIPMEEARPRFVEAAQLIRLALSEREFEWQGEYYKIPRTSIRP
                     HPVSHPERRFFVSSVTPESAEIMAKLGFGMMVIMQNEWPKAAEEIERFRSIAGAVGHV
                     PPPPIILTNVSCAESRDEAHERAMRYLGAKWDSIDNHYHFSDGHLATVKGYEAYGKMT
                     KTYAKMKEDAARQKATEFYVKIQIVGTPDDCIQQIAELRALTGTDHVIADFSYGGMPH
                     EDGELNMRLFADRVVPVLQRDAAFAGVPRRHATAAHEDVFAPA"
BASE COUNT          474 a          710 c         1076 g          499 t
ORIGIN      
        1 cggaaggcgg ggggcgagat cgacctgctg tatatcgact atgcgaagcg gcagggggcg
       61 ggctcgtttg agccggcggc ggcgttcttc caccggttca tccaggtgtg agtcattgcg
      121 agcgtagcgg agccgagatt gcttcgctgc gctcgcaatg acacggggtg cgctcgtggg
      181 cggtcaggct gctggtagtt cggccagcag ccggcccagg gtgggtagcg ggtcgcgggc
      241 gcccatgatg tgcatcatcg accagagcgc ggcgccgttg gtggtgatga cgggcttgcc
      301 gaattcctgt tcccaggcgg gcacgaaaga catggtggga aagttgccgc cggggacgac
      361 gaagcagtcg atctcggggc ggttgatgcg ggcgaaggcg tcgcgggcgt tctcggggcc
      421 caatttgccg atcatgtagg agtcggtggc ggcgaagcct tccagtgcga caacggagag
      481 agcgaatttg ctttcgtagt agtgtctcgc gcgttcaatc acctgctgcg aatagggtga
      541 gacgatggcg atgcgcgtag tgccgagggc gcggatggcc tggccgacgg cttgcgcgga
      601 ggtgacagcg gggacgttgg cggcggcggt gatgcgacgg gtggtgacgg cgtcgtagtc
      661 gtcggcgaac aggctggcgg aggtttgcgc aaggctgatg acctcgacgc gcgcggtggc
      721 gaggaggcgg gactggtagt cgatgtcctc gttcttgctg ggggaaaagg ggccggtgcc
      781 gctggtgagc aggcggccga cgtggaactg caatgagggt ggcagcagat ggctgtactc
      841 gatctctact gtggtgttgg tggagggtat gaggacgccg aagtgatggt tcatgttgtt
      901 cctcccgtgg tttcttgggc gagccgatca taccagctgg tctgatcggc tggggtgaag
      961 tgggcgttgg cgccgctcgg attgggaacg acgtagcagc gcgtggcgcc gatgtagagg
     1021 ggttgcaggc ccagtatggc gtcgggcgac gcggggtcga gaacgcgctg gatgtggcgg
     1081 tagccggtca taccgtggaa gcaggcgatg ccgggccggc aatcgacgag cttggcgacc
     1141 agtacagaga cgccttcagc cagttcattg ggtgagagat cgccggcgcg ggcggtggcg
     1201 cgtttgcaca ggtcggtgaa gccgaagcca tggcctggca gagactggtc gtgttcgggt
     1261 agcagcgtcg ccactgccag cgcgtggcgg gcttcggcac tgagcgtcga ccgcgagaag
     1321 cagggccaga agcggttgcc gcgccgcgcg aagtagtggc cttgcgcgac ggaatagagc
     1381 gacgggttga tgccgacgaa gacgacccga aggccatgcc gcagcaggtc gggcagcgtg
     1441 tcggcaggtg atggtctcga ccccacgcgc gcatcctgac acactttccg caaagacaca
     1501 ggagacggcc atgaaggttg gcgtatcgct gagtatcctc gcgcaggcgg ggcgttccga
     1561 tgtcgcggtc tacaaggagc atctggagct gggggacctg gcggagccgc tcggtttcga
     1621 ttcgatcttt gcgctggagc accatttcac cggctattcg atgtctccgg cgccggtgca
     1681 gctgctgagc tacttcgcgg ggcggacgaa gcgggtgcag ctgggcaccg cggttatcgt
     1741 gctgccgtgg catgatccga tccgggtggc ggagagcatc gcattgctcg atgtgttgtg
     1801 cggcggacgc tgcctgttcg ggttcgggcg cggggcggcg agcgtggaat atgccgggtt
     1861 ccgcattccg atggaggagg cgcggccgcg gttcgtcgag gcggcgcagt tgatccggct
     1921 ggcgctgagc gagcgcgagt tcgagtggca gggcgagtac tacaagattc cgcggacatc
     1981 gatccggccg catcctgttt cgcatccgga gcggcggttc tttgtatcgt cggtgacgcc
     2041 ggagtcagcg gagatcatgg cgaagcttgg gttcggcatg atggtgataa tgcagaacga
     2101 atggccgaag gcggccgagg aaatcgagcg gttccgctca atcgccggcg cggtggggca
     2161 tgtgccgccg ccgccgatca tcctgaccaa cgtttcctgc gcggaaagcc gggatgaggc
     2221 gcacgagcgg gcgatgcgct acctgggtgc caaatgggat tcgatcgaca accactacca
     2281 tttttccgat ggacacctgg cgaccgtgaa ggggtatgag gcgtacggga agatgacgaa
     2341 gacgtacgcc aagatgaagg aagatgcggc gcggcagaag gcgacggagt tctacgtgaa
     2401 gatacagatc gtggggacgc cggatgactg catccagcag atcgcggagc tgcgggcact
     2461 gacggggacg gatcatgtca tcgccgattt ctcctatggc ggcatgccgc atgaggatgg
     2521 cgagctgaac atgcggctgt tcgccgatcg ggtggtgccg gtgctgcaac gcgatgcggc
     2581 gttcgcgggc gtgccgcggc ggcacgcgac cgctgcgcat gaggacgtgt ttgcaccggc
     2641 gtgatggcta gatgcgggtg gaagcgtccc ggatgaagct tttgttgaac ggatccagca
     2701 ggcgcagcag ctcgcggaac catggctgca acacgatgct gagcgggggc gcgatcaga
//
LOCUS       DATGEM010000114        12758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_193861, whole genome shotgun sequence.
ACCESSION   DATGEM010000114 DATGEM010000000
VERSION     DATGEM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12758
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193861"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VKQ27_03565"
     CDS             complement(<1..863)
                     /locus_tag="VKQ27_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88036.1"
                     /translation="MLQLRADPDALAPERLLVFELTGGVMQFTEAVRLVPGLEFLGAE
                     DLDEDDLDDSPVVYLMVPSEAALRNIVTLWRGWQQNGTVPPRFSAWKAMLSQLRNIRP
                     WGPQDRVTAADHDVLVEEYARDAGLTRVEVELVFRRNGEATEADARAGILAVGGGVIA
                     RSRITGAGYHALLADLPGPALADVIARNPAGLAGVEAVLQIRPQSIFQLVPSEEVDGL
                     PAGGAPALDGDPIAAIFDAVPLSQHPRLAGTLSIDDPFDLEGLAVGARKHGTAMASAV
                     VHGDINAAWDRP"
     gene            complement(946..1653)
                     /locus_tag="VKQ27_03570"
     CDS             complement(946..1653)
                     /locus_tag="VKQ27_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY88037.1"
                     /translation="MLSGPPGNGKTSLAEAIAEAVAVPLLTVRYDALVGAYLGETNAR
                     LARLFDYVRTTPCVLFFDEFDAVGKERGDIHETGEIKRVVSFLLMQLDQLPSYVVVVA
                     ATNHAELLDSAVWRRFQLRIDMPLPSRDGAAVLIERFFDAWPEPAGLSAAQVAAQMGP
                     SSFADIFEFCQNIRRRHILSQGGETLKSILAQELRLWKTRIKPESDGQPTEPTAAAPR
                     RGRSDSPKNGRGRTSAT"
     gene            1660..1884
                     /locus_tag="VKQ27_03575"
     CDS             1660..1884
                     /locus_tag="VKQ27_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88038.1"
                     /translation="MTWLHALGAQKVSAVMLLDELAGQMLGGKRQDQAFEPNAWLRFD
                     NEVAAGRASAAQFARRGNGHRSKRPMELVG"
     gene            complement(2455..4449)
                     /gene="rlxS"
                     /locus_tag="VKQ27_03580"
     CDS             complement(2455..4449)
                     /gene="rlxS"
                     /locus_tag="VKQ27_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415233.1"
                     /note="I built this because a sul1 chimera in AMR looks
                     like the C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease RlxS"
                     /protein_id="HLY88039.1"
                     /translation="MADDDHFLPRLGRQRQSSAKRPRRYVGRVLAAAHLARGGAAGLA
                     AKRGFAGSRIGRGSGVGRVLGLRGRHAASSRRRVIVKTNIVRLAGKGASAAAAHLRYL
                     QRDGTTRDGTSGTLYGRDADMIDGKAFAARGSTDRHQFRFIVSPEDGAQYEDLKPLTR
                     RLMARMEEDLGTRLDWVAVDHFNTGHPHSHIVVRGKDMSGADLVIARDYMTIGLRERA
                     AELIDLDLGPRSQREIGQALRAEVEQERLTSIDRTLLKAADVDRVVTAHGRDAFDQSV
                     RAGRLAKLARLGLAEPLGAGRFRLTPDLAETLKRMGERGDIIRTMQRDFSRVRVERAA
                     ADQAIYDPAAPDARPLVGRILARGLADEHADRHYLIVDGVDGRSHYVAIGRGAELAGD
                     NTLLPHGAIVQIEPLRPVIREVDRTVAAVAAAHGGRYDVDAHLRHDPTATEAFAATHV
                     RRLEAMRRMSGIVEREPSGRWIIATDHLERVAAYEAARIRDRPVAITILSVRPLEQLV
                     EADAATWIDRELVSSVPFPVRDAGFGHELREAQARRQQWLVAQGFAEERNGALILRGD
                     MIAALQRRELLRIAGQLADELKLPFREHPEGSRIEGVYRRPIDLVSGRFALIERAHDF
                     TLVPWRPVLDRQVGKSVSGLMRGGGTSWALRPGRSGPSIS"
     gene            complement(4785..5144)
                     /locus_tag="VKQ27_03585"
     CDS             complement(4785..5144)
                     /locus_tag="VKQ27_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88040.1"
                     /translation="MTMVQSVETAGDRVMAALVAGLEIEFGRGAGETLAEHFLEAEES
                     DFLWAARMAKRWLGAYESIDAEGLDLDKIAIMGRCGRCWFVGVSIVDGDGNPQGLISR
                     RHFNSEREARETFAAMH"
     gene            complement(5469..6071)
                     /locus_tag="VKQ27_03590"
     CDS             complement(5469..6071)
                     /locus_tag="VKQ27_03590"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLY88041.1"
                     /translation="MKAVALGIALLATAPAIADPVGHWQAEIAQASVRFGVPSVWIER
                     VMRAESGGLTTLNGLPIISRAGAIGLMQLMPATWADMRRAYGLGSDPHDPHDNIQAGT
                     AYLRILYDRFGYPGLFAAYNAGPGRYADHLASGRPLPNETQAYLAQVTDSAPSPSTTT
                     ANRPPPSLFILLRNTSAGDTSTFSFPSPQNAADPLFAVRR"
     gene            complement(6068..6640)
                     /locus_tag="VKQ27_03595"
     CDS             complement(6068..6640)
                     /locus_tag="VKQ27_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HLY88042.1"
                     /translation="MVERRDLPLFAWGEALRASRARRTRLRRRAALLAIGITGICATI
                     LIPPRPRLVWNASASAPVGLYAVSPGAMLERGDMAIAWPPSAARDLAAARHYLPRNVP
                     LVKRVAGISGDIVCSRKRVVTINSRTAAVRRDADAASRPLPAWQGCMRLRPGMLFLLM
                     VETPDSFDGRYFGPTQARDVIGRAAPLWLR"
     gene            complement(6633..6857)
                     /locus_tag="VKQ27_03600"
     CDS             complement(6633..6857)
                     /locus_tag="VKQ27_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY88043.1"
                     /translation="MDDTATRAQAARETCPFLTSKQAAFHLGLAYSTLKRLRQTGSGP
                     RCRRHGRTWRYHIDDIQTWSATRAHGGDHG"
     gene            complement(7025..7342)
                     /locus_tag="VKQ27_03605"
     CDS             complement(7025..7342)
                     /locus_tag="VKQ27_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HLY88044.1"
                     /translation="MPAIGQVTRQGRGFKGQLRTLSIRTDIEIIPNAKTGDTQPDFRV
                     LAAGVEVGAGWLRRGDMSGKDYVSLSLAAPEFGPRRLYANLGRAAGQDDEDTFAIIWN
                     PAD"
     gene            7795..8124
                     /locus_tag="VKQ27_03610"
     CDS             7795..8124
                     /locus_tag="VKQ27_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88045.1"
                     /translation="MMTQATGKSVEFRFETGTMGLDEIRQRAADMWSDLAFDEAALAR
                     LKRDGLTLDGVRLTGPSPFLLNQEDDHQIAVAIASFVTEPDHAAMLLDLWRVHFMKGL
                     RPGSVAA"
     gene            8263..8853
                     /locus_tag="VKQ27_03615"
     CDS             8263..8853
                     /locus_tag="VKQ27_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HLY88046.1"
                     /translation="MTEELLALTGDIVTAHVSHNAVSTAELPALIRATFEALQKLGEP
                     TEPQEPVQQPAVSIRASVKPDYFVCLEDGKKLTMLKRYLRINFDMSPDDYRAKWGLPK
                     DYPMVAPNYAEKRRTLAHSIGLGQRKAAKSAEGVVTSAVEPVEPVAEPIVEAVKTSRK
                     KLGIAAAKATAGARPDGGIPAPRSRKLSKPKALKAS"
     gene            complement(8959..9831)
                     /locus_tag="VKQ27_03620"
     CDS             complement(8959..9831)
                     /locus_tag="VKQ27_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HLY88047.1"
                     /translation="MSSPARTDVYARVTNAIIAAIETGAGTWRMPWHHEGADATRPTN
                     VASGKPYRGINIVSLWAAACTTGYASGIWGTYRQWQALGAQVRRGEQASVGIFWKEVD
                     RRDVDNGSDDDGDHHPRLFARAFSLFNADQVDGYAPEPGAILPESERLATADQFIAAL
                     GIDTVYGSGSAYYHIAEDRIHMPDFAAFHDAHGFFATYIHECAHACGAAHRLDRNFSA
                     KWTRHALAMEEAVAELTASFLLADLGIAHEPRPDHAAYIASWLDLLKDQPRAIFTAAS
                     KAQAAADWMHKQQR"
     gene            complement(10698..11069)
                     /locus_tag="VKQ27_03625"
     CDS             complement(10698..11069)
                     /locus_tag="VKQ27_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY88048.1"
                     /translation="MILIDTSVWVDHLRRGDLALSDLLTRSQVLTHPFVIGELALGSL
                     RQRDIVLGALRDLPQAVVAGDDEVQAFIDRHTLFGIGIGYVDAHLLAGTLLTSGARLW
                     SRDKRLSDAAGRLGIAADRDH"
     gene            complement(11066..11263)
                     /locus_tag="VKQ27_03630"
     CDS             complement(11066..11263)
                     /locus_tag="VKQ27_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLY88049.1"
                     /translation="MRTTLALDDELVAKAQAFTGLTEKSSLIREALKALIARESALRL
                     ARLGGTEPEILAAPRKRPEQP"
     gene            complement(11423..12013)
                     /locus_tag="VKQ27_03635"
     CDS             complement(11423..12013)
                     /locus_tag="VKQ27_03635"
                     /inference="COORDINATES: protein motif:HMM:NF021581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HLY88050.1"
                     /translation="MPTDPANPDCVHIDRLAPWLAIAVDAQGREHAVLSDGWHHIRFD
                     VEQGRLAGEQAVLLRYRVQGVASAEARLLPLRRFLYLYRHHQFGRSLYPPDPRIDRWI
                     EMLRAHDALLAGASQREIAAALYGADRIARDWGEASDSLRSRIRRLVRDAHAMARGGY
                     RTLIRRRTTAVKSFDVGSSVQQPAAVPYNSLSGAGG"
     gene            complement(11925..12380)
                     /locus_tag="VKQ27_03640"
     CDS             complement(11925..12380)
                     /locus_tag="VKQ27_03640"
                     /inference="COORDINATES: protein motif:HMM:NF040446.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6499 domain-containing protein"
                     /protein_id="HLY88051.1"
                     /translation="MDVLSGNNRGFHCHSTTSVFICTSHGRPVIPPIGHNSPPDWRDA
                     TSYERLCGIDRAGLMWEWLRRDRDYVAWYVQASKATCGVDPQPLRWGLHFRRASGPRG
                     PGRPDHLACRFRSRDDCCHRSADRSRQSRLRAHRPARAVAGDRGRCAGA"
     gene            12556..>12758
                     /locus_tag="VKQ27_03645"
     CDS             12556..>12758
                     /locus_tag="VKQ27_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HLY88052.1"
                     /translation="MGKQTMMMRLMRIVEATTSIDDLAVALAAVTTQLGFQHFALCHH
                     VDAVAAGGHVIRLHNYPAQWVDY"
BASE COUNT         2296 a         4012 c         4281 g         2169 t
ORIGIN      
        1 ggacggtccc acgccgcgtt gatgtcaccg tgaaccacgg cgctagccat cgccgtgcca
       61 tgcttgcgcg cgccgacagc cagcccctcc agatcgaagg gatcgtcaat actgagcgtg
      121 ccggcgagcc gtggatgctg cgacaagggc accgcatcga agatcgcggc gatcggatcg
      181 ccatcgagcg ccggtgcacc gccggccggc agtccatcaa cttcctctga cggaaccagc
      241 tggaagatgc tttgcggccg gatctgaaga acggcttcga cgccagcgag cccagccggg
      301 ttacgcgcga tcacgtcagc gagcgcaggg cctggcagat ccgccagcaa cgcatggtag
      361 ccggcgccgg tgatccgcga gcgtgctatg acaccacccc cgaccgctag gatgcccgcg
      421 cgcgcatcgg cctcggttgc ctcgccattt cggcggaaca cgagctcgac ctcgacgcgg
      481 gtcagccccg cgtcacgagc atattcttcg acaagaacat cgtggtccgc tgccgtgacg
      541 cggtcttgtg gcccccacgg gcggatgttg cggagctgcg agagcatcgc cttccaagcg
      601 ctgaacctcg gtggcactgt gccgttctgc tgccaaccac gccacaaggt gacgatgttc
      661 cgcaatgccg cctcgcttgg gaccatcaga tagacaacgg gactgtcgtc gaggtcgtcc
      721 tcgtcgagat cttcggcgcc cagaaattcg aggccgggca cgagacgcac ggcttcggtg
      781 aactgcatca ccccgccggt aagttcgaag acgagcagcc gttctggcgc gagcgcatcc
      841 gggtcggcgc gcagctgaag catcggggtg ttcgcgtcga gttgggccgc gagacctgcg
      901 agcttctgac cggcgggcgt accggcctgc tgtgccgggt caagcttacg tggccgaggt
      961 gcgcccccgc ccgtttttcg gggaatcgct gcggccgcgt cgaggcgcag cagcggttgg
     1021 ttcggtaggt tgaccatcgc tctccggctt tatgcgtgtt ttccagaggc ggagctcctg
     1081 cgcgaggatg ctcttcagcg tctctccccc ctgggacagt atgtgtcgcc gccggatgtt
     1141 ttggcagaac tcgaagatgt cagcaaagct cgatggcccc atttgggcgg cgacttgggc
     1201 ggccgacaag ccggcgggct caggccacgc atcgaaaaag cgttcgatca gcacggccgc
     1261 accatcgcgt gacggcagcg gcatgtcgat acgaagctgg aagcggcgcc aaaccgcact
     1321 gtccagcaac tcggcgtggt ttgtcgcggc caccacaacg acatagctag gcaattggtc
     1381 gagctgcatg agcaggaagg acacgacacg cttgatctca ccggtctcgt gaatgtcgcc
     1441 gcgctctttc ccgaccgcat cgaactcatc gaagaagagg acgcagggcg tggtacgaac
     1501 ataatcaaac aggcgcgcga gccgggcatt agtctcgccg agataggcac cgaccaaggc
     1561 gtcataccga acggtgagca gcgggaccgc aaccgcttcg gcaatggcct cggcgagcga
     1621 cgtcttgcca tttcctggcg gtcccgacag caagacgcgg tgacgtggct gcatgccctg
     1681 ggcgcgcaga aggtcagcgc ggtgatgctc ctcgacgagc tggcgggtca gatgctcggc
     1741 gggaagcgtc aagatcaggc tttcgagccc aacgcgtggc tccgattcga taacgaagtc
     1801 gcggccgggc gcgccagtgc ggcgcagttc gctcgacgcg gtaacgggca ccgaagcaag
     1861 cgcccgatgg agcttgtcgg ctaggacgtg gtggcttttg gcgcgttcgt cggcgaccag
     1921 tgcctcgaca gtcgtggtca atgtcgaacg gtcgccagac gctcctgcct tcaccaacga
     1981 aactaggaga tcacttctgg ccatcaaatc ctacacacgc cccttcgccc tctgggcgaa
     2041 gcgtaacacg agcccccctc cgtgtgaatc cctgataggc tgatacgccc aattgttgaa
     2101 cgtcctgccg atatggcgtc gtcctcaccc ctggggggcg ccctcggtaa gcgcgatcgc
     2161 ggtgcccgaa ctgtaagcct gaaagtctag tagcaacgat gagatcgagc cgtcgggctc
     2221 caaaaactcg tgattggcgc cggggatcag gtcgggatcg agcgggatgc gagcgctcgg
     2281 attgaaaatt cggattgaaa ataagattat agaaaagctt gggtatggat cgtggcgaac
     2341 gtgtcaggca gcggcgcccg cgaacatccg ctgtcacggg tccgctgcct ggaagctgtc
     2401 agtctccaat cggccagaac tccccgtaag cagcccgccc acaattgcgc cagctcaaga
     2461 aatgctcggt ccactccgtc ctggtctcag cgcccagctg gttccgccgc cgcgcatcag
     2521 accggagacc gatttgccga cctggcgatc gagcaccggc cgccacggca ccagcgtgaa
     2581 gtcatgggcg cgctcgatca gcgcgaaccg cccgctgacg agatcgatcg ggcgccgata
     2641 gacgccttcg atccggctgc cttcgggatg ctcccgaaaa ggaagcttga gttcatcggc
     2701 aagctggcct gcgatccgca acagctcgcg tcgctggagg gctgcgatca tgtcgccgcg
     2761 gaggatcaag gcgccgttcc tttcctcggc gaaaccctgc gcgaccagcc actgctggcg
     2821 gcgtgcctgc gcttctcgca gctcatggcc gaacccggcg tcgcgaacgg gaaagggcac
     2881 gctcgaaacc agctcccgat cgatccaggt cgcggcatcg gcttcgacca gttgttcgag
     2941 cggccgcacc gacaggatgg tgatggcgac aggacgatca cggatccgtg ccgcttcata
     3001 agccgctaca cgctcgagat ggtcagtcgc gatgatccag cggcctgacg gttcgcgctc
     3061 gacgatgccg gacatgcgtc gcatcgcttc aagtcgccgg acgtgcgtcg cggcaaaggc
     3121 ctcggtggcc gtcggatcgt gtcgcagatg ggcgtcgaca tcatagcgtc cgccatgggc
     3181 ggcagcgacc gcggcgacgg tccggtcaac ctcgcgtatc accggccgga gcggctcgat
     3241 ctgcacgatc gcaccgtgtg gcagaagggt gttgtcaccg gccagctcgg cgccccttcc
     3301 gatcgcgaca taatggctgc gcccatcgac cccgtcgacg atcaggtaat ggcggtcggc
     3361 atgctcgtcg gcgaggccgc gcgcaaggat gcgtccgacc agcggccggg cgtcgggagc
     3421 ggctggatca tagatcgcct ggtcggcggc cgcccgctcg acgcgaaccc gggaaaaatc
     3481 gcgctgcatg gtgcggatga tgtcgccgcg ctcgcccatg cgtttcaatg tttcggcgag
     3541 gtcgggcgtg aggcgaaagc ggccggctcc gagcggctcg gccaggccca ggcgcgccag
     3601 cttggcgaga cgtccggcgc gcacgctctg gtcgaaagcg tcgcggccgt gcgccgtgac
     3661 cacccgatct acgtccgccg ccttgagcag cgtgcgatcg atcgaggtca gccgctcctg
     3721 ctctacctct gctcgaagcg cctgcccaat ctcgcgttgg ctacgcggcc cgaggtcgag
     3781 gtcgatcagc tcggcggcgc gctcgcgcaa tcctatggtc atatagtcgc gcgcgatgac
     3841 gagatcggcg ccggacatgt ctttgccgcg cacgacgata tggctgtgcg gatggccggt
     3901 gttgaaatgg tcgaccgcga cccagtcgag cctggttccg agatcttcct ccatgcgcgc
     3961 catcagtcgc cgggtcagcg gcttcagatc ttcatattgt gcgccgtcct ccggcgagac
     4021 gatgaagcgg aactggtggc gatcggttga gccccgcgcc gcgaatgcct tgccatcgat
     4081 catatcagca tcgcgaccgt agagcgtgcc gcttgtccca tcgcgtgtcg tgccgtcgcg
     4141 ctgaagatag cgtaggtgag cggcggccgc cgacgcgccc ttgccggcga gccggacgat
     4201 gttggtcttg acgataacgc ggcgcctgct cgacgcggca tggcggcctc gaagaccaag
     4261 cacgcgcccg acgcctgagc cacgtccgat ccggctgccg gcgaaacctc tcttcgcggc
     4321 caacccggcc gcaccgccgc gcgcgagatg ggcggcggcg agcacccgcc caacatagcg
     4381 acgggggcgt ttggcgctcg attgccgctg acggccgagg cggggcaaga agtggtcgtc
     4441 gtcggccatc atcctggttt cctgaagaag cgagaaggcg aggatgccgc agccggactc
     4501 ccaagaaaac cggtgccatc ccgcgtggct gaaaaccggg gatttcatgg aagacggcac
     4561 tggtcgcttt gagggagtgc cgcaaagcgg ccgaaaaaac gatgtgcagg caagggctta
     4621 tggagcgcca gtgccatagc tttcatcttg ccttacgccg ccctcacgcc ccgaccgccc
     4681 aaaatgccca tgatccgccc atcgcccacc ttcgagccga ggcaaaaaga agccgggaca
     4741 cgatgcctgt tcggcaccgc gacccggcct atgcgcatcc tcgatcaatg catcgccgca
     4801 aacgtctcac gagcctcacg ctcgctgttg aaatgccgcc ggctgatcaa tccctgcggg
     4861 ttgccgtcgc catcgacgat gctcacgcca acgaaccagc acctgccgca tcgccccatg
     4921 atcgcgatct tatcgaggtc gaggccttcc gcgtcgatgc tctcataagc gcccagccaa
     4981 cgcttcgcca tccgcgcagc ccacaggaaa tcgctctcct cggcctcgag aaaatgctca
     5041 gccaaagtct cgcctgcgcc ccgaccgaac tcgatctcga gccccgcgac gagtgccgcc
     5101 atcacgcggt cgccagctgt ttcgacggac tgaaccatgg tcatgatcgt ctcctgcttg
     5161 atgtccccgt cattgaggac agtcatccgg agacgggcgg gggagtggct gtcagggccg
     5221 ctgcaggcaa agccggaagc gccgcgcgag cggcggcgga ggagccgatt ttgttgcggg
     5281 aaaacaaaca ggcgcgcgtg cgaagcacgc tcctgttcgc ggcaaaatgg gaggggccgc
     5341 cggccttgca cagccgcttc gccgtccgtc atgctgggcc ttaccgagac gagcccctcc
     5401 cacgcttgcc gtcgcggcac accggctgct ccaggcgccc ttcggccgtt cgacgggcgg
     5461 catcagcgct atcggcgcac cgcaaacaac gggtccgctg cattctgcgg agagggaaaa
     5521 gagaaggtgc tggtgtctcc agcgctggtg ttccgcaaca ggatgaagag cgaaggtggc
     5581 ggtcgattgg ctgtcgtggt cgacgggctc ggggcggaat ccgtcacctg cgcgagatag
     5641 gcctgcgtct cattggggag cgggcggccc gatgcgagat ggtcggcgta gcggccggga
     5701 ccggcattat aggcagcgaa caacccaggg taaccgaagc gatcgtacag gatgcgcaga
     5761 taggcggtgc ctgcctggat attgtcgtgc gggtcatgtg gatcgctgcc aagaccgtag
     5821 gctcgacgca tgtccgccca ggtcgccggc atcagctgca tgagcccgat cgcgcccgcg
     5881 cggctgatga tcggcaggcc gttcagggtg gtgagcccgc cgctttcggc gcgcattacg
     5941 cgttcgatcc agaccgacgg cacgccgaac cggaccgagg cctgtgcgat ttcggcctgc
     6001 caatgtccga ccgggtcggc gatggcggga gctgttgcga gcagcgcgat gccgagcgcg
     6061 accgctttca tcggagccac agcggggccg ccctgccgat gacatcgcgc gcctgcgttg
     6121 gtccgaaata gcgcccgtcg aacgagtccg gtgtctcgac cataagcagg aagagcatgc
     6181 ccggtctcag tcgcatgcag ccctgccatg caggcagcgg ccggctcgcg gcgtcggcgt
     6241 cacgacgcac ggcggcggtc cggctattga tggtcacgac gcgtttgcgc gaacagacga
     6301 tgtcgccgga aatgccggca acgcgcttca cgagcggcac gttgcgcggg agatagtgac
     6361 gtgctgcggc aaggtcgcgc gcggcggacg gcggccaggc gatcgccatg tcgccgcgct
     6421 cgagcatcgc gccaggcgac acggcgtaga ggccgacggg cgcgctggcg ctggcattcc
     6481 agaccagccg ggggcgcggc ggtatgagga ttgtcgcgca gatgcccgtg ataccgatgg
     6541 caagaagggc tgcgcggcgg cgcagtcgtg ttcgcctggc gcgactggct cggagcgcct
     6601 cgccccatgc gaaaagcggc aggtcgcggc gctcaaccat ggtcgcctcc gtgcgctcgg
     6661 gtcgccgacc atgtctggat gtcgtcgata tgatagcgcc aggtgcggcc atgccggcgg
     6721 catctgggac cgcttccggt ctgccgcagg cgcttcagcg tcgaataggc aaggccgagg
     6781 tggaaggcgg cctgcttcga ggtcaaaaag gggcaggtct cgcgtgcggc ctgcgcccgt
     6841 gtcgctgtat cgtccatcgt gtcggtccat ctgcgctggc gcaaaatgcg ccaacggcac
     6901 ggcgcggaat ggatcggacg ggccgtcttc ggcagggtcg caacgggcta caggcgtttt
     6961 cgaccccctc acgtgggggc gcgcggcgat gccccgcgtc ctcgaaggac gcggggcgga
     7021 tcgctcagtc ggcggggttc cagatgatgg cgaaggtgtc ttcgtcatcc tgtccggcgg
     7081 cgcggcccag attggcgtag agtcgtcgcg gaccaaattc gggggcggcg agtgacagcg
     7141 agacataatc cttgccgctc atgtcgccgc gccggagcca cccggcgccg acttcgacac
     7201 cggccgccag cacgcgaaaa tcgggttggg tatcgcccgt ttttgcgttg gggatgatct
     7261 cgatgtcggt acggatcgaa agcgtccgga gctggccttt gaaaccccgt ccctggcggg
     7321 tgacctgacc gatggctggc attgttcgtc tcctgatgat cgcgcccgag cctgtcccgg
     7381 acgatgggcc gaccggagga ccgggcaaag caggaccacg aaccggacgg ccggagcgaa
     7441 agcggaggac tggagggcga cgcttatttg gagcgtgcag cgaccgcgag gagccggcgt
     7501 gacggggccc gacagggtcc ccgcgccggc ggggaaataa gcgccagccc gatggtttcg
     7561 tgccgtcctg cgtccggtcc aggtcatggg cccaaggagc cggacaggat aggatggtga
     7621 gcaaggtcag agcgattccc cgcggtcgat gcggcaagga cagaggctca aggcagcact
     7681 actggacaat cctgtcttcg tacaaggcga gtatgcgtcg gttgaacggg gcccgggccc
     7741 gcgatagttg ccgggatcgg gatgggtgtg cgggggcatg cgcggaccag cgacatgatg
     7801 acgcaagcga cagggaaaag cgtggaattc cggttcgaaa cagggacgat gggactggac
     7861 gagatccggc aacgcgcggc tgacatgtgg tcggacctcg ccttcgacga ggcggcgctg
     7921 gcccgtttga agcgcgatgg cctgacactt gacggcgtgc gcctgacggg gccgagcccc
     7981 tttctactga accaggagga cgatcaccag attgcggttg cgatcgcgtc cttcgtcacc
     8041 gaacccgatc atgccgcgat gctgctcgat ctgtggcgcg tgcacttcat gaaggggctt
     8101 cgcccgggca gtgtcgcggc ctgagcgacg cctgacgcat gatcgtcgct gccctttact
     8161 ggccttccac aaagaagcgc cgaagaaggg cgatatggct ttgcttgttc atgcgtccaa
     8221 tgtcggtatg aagtggcgct catcgctcgt gcgaggatcg tcatgactga agaattgctt
     8281 gcgcttaccg gcgatatcgt gaccgctcat gtcagccaca atgctgtcag cacggccgaa
     8341 ctgcccgcgc tgatccgcgc aaccttcgag gcgctgcaga agctcggtga accgaccgaa
     8401 ccgcaagagc ccgtgcagca gcccgccgtt tcgatccgtg cgtcggtcaa gcccgactat
     8461 ttcgtctgcc tcgaggacgg gaagaagctg accatgctca agcgctatct gcgcatcaac
     8521 ttcgatatgt cgccagacga ctatcgcgcg aaatggggcc tgcccaagga ttacccgatg
     8581 gtcgcgccca attatgccga gaagcgtcgc acgcttgccc attcgattgg gctgggtcaa
     8641 cgcaaggccg cgaagagcgc ggaaggggtg gtgacgagcg cggtcgaacc cgtcgagccg
     8701 gtcgccgaac cgatcgtcga ggcggtgaag acgagccgca agaagctcgg cattgcggct
     8761 gccaaagcca ctgctggcgc gcgtccggac ggtgggatac cggctccccg gtcgcgcaag
     8821 ctgtcgaagc cgaaagcgct caaggcaagc tgacgaccat ggggccgctg atgcgcgggc
     8881 gtcagcccgc atatcgacgg cgcacgaccg agcggtcgag ccggaaacgg ctcggccgct
     8941 cggacatggt cgcgcacgtc agcgctgctg cttgtgcatc cagtcggccg ctgcttgcgc
     9001 cttgctggcg gcagtgaaga ttgcccgcgg ctgatccttg agcaggtcga gccaggaggc
     9061 aatgtaagct gcgtggtcag gtcgcggttc atgcgcgatc ccgagatcag cgagcaggaa
     9121 ggatgcggtg agctcggcca ccgcttcttc catcgcgagg gcatgccgcg tccacttcgc
     9181 gctgaaattc cggtcgagcc tgtgggcagc gccgcaggca tgggcacatt cgtggatata
     9241 cgtggcgaaa aagccgtgcg cgtcgtggaa ggcggcgaaa tccggcatgt ggatgcggtc
     9301 ttcggcgata tgataatagg cgctgcccga gccgtagacg gtgtcgatgc ccagtgctgc
     9361 gatgaattga tcggcggtcg cgagccgttc gctctcgggg aggatggcac cgggctcggg
     9421 tgcgtagccg tcgacctgat cagcattgaa caggctgaac gcccgggcga agagccgcgg
     9481 atgatggtcg ccgtcatcgt cgctgccatt gtcgacgtcg cggcggtcga cctccttcca
     9541 gaagatgccg acgctggctt gttcgcccct gcggacctgg gcgccgagcg cctgccactg
     9601 acgataggtg ccccagatcc cgctcgcata gccggtcgtg caggctgcgg cccaaagcga
     9661 cacgatgttg atgcctcggt acggcttgcc gcttgcgacg ttggtcgggc gggtggcatc
     9721 ggcgccttca tggtgccacg gcatgcgcca ggtgccggcg ccagtctcga tcgccgcgat
     9781 gatggcgttg gtgacgcggg catagacgtc tgtacgggct ggggatgaca tgatctttcc
     9841 tccgttcggt gccggggacc atccccggcg gcggaggccg cttggcgtcg ggcacaggtg
     9901 ggctcgcgca cccgcgaggg ccgtagcgaa gcgaaggacg gcgtgagccg ttgcgcgggt
     9961 tcgccgaccg gcgcaggact ccgccagcag ccgggcggtc accggggcga aggatcgatc
    10021 gctggtacaa acagccgcca gacggcgaag ctctgccgcg agcgcgcgac gctgaagatc
    10081 cgccatacag cgctcgcaca agcaagcgtc cggcgtcgcg gctctctccc atgtcggccg
    10141 ggatcgtgtc ccgggtggtg gtaagcgacg caggcgttgg ggcagcggtt cggttcagtc
    10201 agcgcgatgc gcggacattt agccggccgg aagccgaggc cggcgatcgg cgcatcgttg
    10261 cggaggcaga tgcgcgcgtg acgatcagga tgccgcgcga accctgtctg gcttcacggc
    10321 gtcgtttctg atccccgggc gtcgctggcc catcgatggc ctggcggcgg atcagggtct
    10381 gcggggcatc agggtccgat attcgccgcg cgccatggcg tgggcgtcgc gtgccagcct
    10441 gcgtatccgc gaacgaagcg aattcgaggc ctctccccgg tcgcgcgcaa ttctgtctgc
    10501 cccatggcct atcgtctgcc atggcggtcg cggcactttc tgtccggtct caaggattgg
    10561 tgtaggtaac tcttctctag gcggttccgt ctaggccaaa tcagatagtt acatcacgat
    10621 cgccgcccaa aatcgggccc ccggcgcatg agacgggcta aggttgtgcc tcgattgcga
    10681 gggacgcggg gactggttca atgatctcga tcggcagcaa tccccaagcg accagcagca
    10741 tcgctcaacc gcttgtcgcg cgaccagagc cgggcaccag aggtgagcaa cgtgcccgcc
    10801 agcagatgcg catcgacata accgataccg atgccgaaca gggtatggcg gtcaataaat
    10861 gcctgcacct cgtcgtcgcc ggcgaccacc gcttgcggca gatcgcgcag tgcacccaga
    10921 acaatgtcgc gctggcgcag gctccccaag gccagttcgc cgatcacgaa ggggtgggtc
    10981 agcacttggc ttcgagtgag aaggtcggaa agggccaaat ctccgcgacg caaatgatcg
    11041 acccaaaccg aagtatcgat taatatcatg gttgttcagg tcgcttgcgg ggcgcggcaa
    11101 gaatttcggg ctctgtgccg ccgagacggg caagacgcaa agcgctctcc cgggcgatca
    11161 acgccttcag cgcttcgcgg atcagcgagg atttttcagt cagaccggtg aaagcttgcg
    11221 ccttcgctac caattcatcg tcaagcgcga gcgtggtgcg cataggtctt ctccatcgct
    11281 gagcacgaaa tatggcatca aatgatgcgc atttcaatgc cggcgatgag aatttttacg
    11341 gccgctatcc cgcgcatggc gaggcatgct gagtggtcgg ttctcgggca attgctgcca
    11401 agctaagttc gcaaggatat gatcacccgc cagccccaga tagcgaattg tacggcactg
    11461 cagccggctg ctggaccgat gatccaacat cgaagctctt cacggcggtc gttctgcggc
    11521 ggatcagagt ccgatatccg ccgcgcgcca tggcgtgggc gtcgcggacg agtctgcgga
    11581 tccgcgaacg cagcgaatcc gaagcctctc cccagtcgcg cgcaattctg tccgccccat
    11641 agagcgccgc cgcgatctcc ctctggctgg cgcctgccag cagcgcgtca tgggcgcgca
    11701 gcatctcgat ccagcggtcg atgcggggat ccggtggata gagcgagcgt ccgaattggt
    11761 gatggcggta gaggtacaga aagcgacgca gcggcagcag ccgcgcttcg gcagaagcca
    11821 cgccctgcac ccggtagcgc agcaggacgg cttgctcgcc cgccagccga ccttgttcca
    11881 cgtcgaagcg gatatgatgc cagccgtcgg acagtacggc atgttcacgc ccctgcgcat
    11941 cgaccgcgat cgccagccac ggcgcgagcc ggtcgatgtg cacgcagtcg ggattggcgg
    12001 gatcggtcgg cactgcggtg acagcaatcg tcccgggatc gaaatcggca tgccagatga
    12061 tccgggcgtc cggggccgcg aggcccggat gctcggcgaa agtgcaaccc ccaccggagc
    12121 ggttggggat cgacgccgca agtcgccttg ctcgcctgga cataccaggc gacgtagtcg
    12181 cgatcacggc gaagccattc ccacatcagg ccggcgcgat cgatgccgca cagccgctca
    12241 tagctggtcg cgtcgcgcca gtccggaggg gagttgtgcc cgattggtgg gatcacaggc
    12301 cgaccatgac tggtacagat gaatacgctg gtagtagaat ggcaatgaaa gccccgatta
    12361 ttgcctgata atacgtccat attgtttgaa tgttgttccg gcttttacaa tataaaaagc
    12421 catgatagga cgtgtccatt agtggattcg ccggcatgtg gcgtgagaac ccgctgtccg
    12481 gcgcatggca tggggaaggg cgatcgctgg gggcgcggtg gcttgagaca ccgcctgcgg
    12541 tcgtgaagcg gcccgatggg caaacagaca atgatgatgc gcctgatgcg catcgtcgag
    12601 gcgacgacca gcatcgacga tctcgccgtg gcgctggccg cggtgacgac ccagctcggc
    12661 ttccagcatt tcgcgctctg tcatcatgtc gatgccgtcg cggcaggcgg ccacgtgatc
    12721 cggctgcaca attatccggc gcaatgggtg gactatta
//
LOCUS       DATGEM010000115         6376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_443967, whole genome shotgun sequence.
ACCESSION   DATGEM010000115 DATGEM010000000
VERSION     DATGEM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443967"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VKQ27_03650"
     CDS             complement(<1..233)
                     /locus_tag="VKQ27_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88053.1"
                     /translation="MPLQPTIRRSLFASSLALIGLIGLIAFFAYLIISAAELRRDRLA
                     ADRWHDHTLEAIIAAGDVDAALHDRSEVPLTDEA"
     gene            complement(372..1085)
                     /locus_tag="VKQ27_03655"
     CDS             complement(372..1085)
                     /locus_tag="VKQ27_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HLY88054.1"
                     /translation="MDRREWGRVGGAVAMAVVAMVGCDLVANLLVPSSYPVRPGYAVP
                     GVAEPAVNLAALQRSWPAGMSEPGSRPRLIGYMSRIERAATPTLAAPEAPAPPERDLG
                     VRLAAGNVEKGRQTAQICGTCHSLDRDGPNRIGPDLWGVVGRNVASHPGFSYSSAMKS
                     QSGNWTYERLDHFLAGPGRAVPGTKMTFMGIRDPEARASVLAYLSTLNTSPPPFPPPH
                     AQPEQGMAQHAKSAQTPSS"
     gene            complement(1089..1463)
                     /locus_tag="VKQ27_03660"
     CDS             complement(1089..1463)
                     /locus_tag="VKQ27_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HLY88055.1"
                     /translation="MNHTPSPAPMQAAPPASEGHAGGQQHPIGLYLKVWGYLFVLSTM
                     SYLVDYFHVQGGLRWFLIVTFMVLKAGLIVSIFMHMAWERISLIYAILVPPLCLATLV
                     ILMAIESDYTFWTRILFFDRGG"
     gene            complement(1476..2174)
                     /locus_tag="VKQ27_03665"
     CDS             complement(1476..2174)
                     /locus_tag="VKQ27_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HLY88056.1"
                     /translation="MAQAIETNAAATGTEPQGLGGFAADWSADQEVFRHVHWGKAMMW
                     IFLLSDTFIFSCFLTSYMTMRASATLPWPDPSKVFALTIGGQTIPLILIAIMTFVLIS
                     SSGTMAMAVNFGYRRNRRVTAALMGATALLGASFVSMQAFEWHKLIFEEGVRPWTNPM
                     GAPQFGASFFMITGFHGLHVSAGVIMLAIVASKVLRGHYDRRADYSAVEIAGLYWHFV
                     DLVWVFIFAFFYLW"
     gene            complement(2191..2892)
                     /locus_tag="VKQ27_03670"
     CDS             complement(2191..2892)
                     /locus_tag="VKQ27_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HLY88057.1"
                     /translation="MSLLAALTEKPWLAPGPAGDRPDRSTPATGLAVFLCVVTMLFLL
                     LTMAYLMHMSGHGTASGETGGHWHSMPKPPLLWANSGILLLSSFAWQAARRAARRGDA
                     AALRSALIVAGALGLAFLSGQLFVWHQLREAGYFLSSQPGFCLIGLGTPDRSFQTLHP
                     FLTGSPAIAFFYLITALHGLHLLGGVAVWGHATSQVLRGGSIAAVRQQVDLCGLYWHF
                     LLLVWLWMFGLFLLT"
     gene            complement(2889..4634)
                     /locus_tag="VKQ27_03675"
     CDS             complement(2889..4634)
                     /locus_tag="VKQ27_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLY88058.1"
                     /translation="MATVTLADDMRPLHHPKTFIGKYIWSQDHKVIAIQYSLTAISIG
                     VVALVLSALMRLQLGFPGTFSFIQPDHYLQYVTMHGMIMVVYLLTALLLGGFGNYLIP
                     LMVGARDMVFPFVNMLSYWVYLLSVLVLVSGFFVPGGPAGSGWTLYPPQAILDGTPGH
                     QWGIITMLVSLAIFIVAFTMGGLNYVTTVLQARTKGMTLMRMPCSVWGIFVASIMGLL
                     AFPALFVAAIMMLLDRIAGTSFFMPSIVSMGQQTGYQGGSPILFQHLFWFFGHPEVYI
                     VALPAFGIVSDLISVHARKNIFGYRMMVWAIIVIGALSFFVWAHHMYVSGMNPYFGFF
                     FATSTLIIAIPTAIKVYNWLLTLYRGNIHLRVPMLFAIGFLFTFIHGGLSGLFLGNVS
                     VDLPLSDTFFVVGHFHMVMGVSPILVIFGAIYHWYPKITGRMWNDMLGKIHFWITFLG
                     VYAIYLPMHYLGILGVPRRYYALGPTAFIPPSAHLANEGITIAALIVFVAQGLFFFNM
                     IWSFFRGPKADPNPWGAASLEWQTPDTPPKHGNWGPRLPTVYRWAYDYSVPGAAQDFI
                     PQNVPPGDADRHHPT"
     gene            complement(4634..5479)
                     /locus_tag="VKQ27_03680"
     CDS             complement(4634..5479)
                     /locus_tag="VKQ27_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLY88059.1"
                     /translation="MLGSIAFHLLSPWWRTPLASNWGSIDSALSITLWICAVAFVALN
                     LFLAYALFRYRYRDGHRAHYEPENTRLEKRLTFWTAIGIAGMLAPGLLAWNRFVNVPK
                     DASIVEAIGQQWQWSYRFPGPDGVLGTSDVRNITPDNPFGLNPQDPYGRDDRLIEGSE
                     LHLPLNKPVKMLLRSKDVLHDFYVPEFRARMDMVPGIITYFWLTPTRPGTYEVLCAEL
                     CGVGHYMMRGTVIVEPEPAFRAWLAKQPTYAQLAAASAPAGTELAQAKTTTIPTAADQ
                     PRGKR"
     gene            5934..>6376
                     /locus_tag="VKQ27_03685"
     CDS             5934..>6376
                     /locus_tag="VKQ27_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLY88060.1"
                     /translation="MLVTLQSIRRQWPTALRLLVEPIRELRPLGLGYLPVPGAMMNFI
                     HCSMQHQRPNPPSGHALSAVRPAPAFHRSATTNQRGIPLMATNAYGAHAADTPLEPIM
                     IERRAPGPHDVQIEIAYCGVCHSDLHTVRSEWGGTVYPCVPGHEIV"
BASE COUNT         1276 a         1947 c         2116 g         1037 t
ORIGIN      
        1 gcctcgtcgg tcaggggcac ttcggaacga tcgtgcagcg cggcatcgac gtcgcctgcg
       61 gcgatgatcg cctccagcgt gtgatcgtgc cagcggtcgg cagccagccg gtccctgcgt
      121 agttcggcgg cgctgataat gagataggcg aagaaggcga tcaggccgat caggccgatc
      181 agcgccagcg acgaggcgaa cagcgatcgg cgaatggtcg gttgcagcgg cacttcacgc
      241 ggcctcgact atgccggaaa cacagtaagg aaatttagtt aacaaatcct tatcatcgcg
      301 tcggcggaca tcagggatgg agggaggatg agggttctga tcgggacacc gtcgtgatgc
      361 agccccgaac ctcatgatga tggagtctgc gccgattttg catgttgcgc cattccctgt
      421 tctggctgcg cgtggggcgg cgggaaaggg ggcgggcttg tgttcagcgt gctcagatag
      481 gcgagcacgc tggcacgggc ttccgggtcg cggatgccca tgaaggtcat cttggttccg
      541 ggcaccgccc gaccggggcc tgcgaggaaa tggtcgagcc gctcatacgt ccagttgccg
      601 gattggctct tcatggcgct ggaataggag aagcccggat gcgaggcaac gttgcggccg
      661 acgacgcccc aaaggtcggg cccgatgcgg ttcggaccat cgcgatcgag actgtgacag
      721 gtgccgcaga tttgcgcggt ctgtcggccc ttttcgacat tgcctgccgc gagccggacg
      781 ccgagatcgc gttcgggtgg cgccggtgcc tcgggggccg caagcgtcgg cgtcgccgcc
      841 cgctcgatcc ggctcatata gccgatcagc ctcggccggc tgcccggttc gctcatgccg
      901 gccggccagc tgcgttgcaa cgccgcgagg ttgacagccg gctcggccac accgggcact
      961 gcatagccgg gccggaccgg atagctgctc ggcacgagca ggttggcgac caggtcgcac
     1021 cccaccatcg cgacgaccgc catcgccacc gcgccgccga cccgccccca ttcgcgccgg
     1081 tccatcgctc agcctccgcg atcgaagaag agaatgcggg tccagaaggt gtagtcggac
     1141 tcgatcgcca tcaggatcac cagcgtggcg aggcacagcg gcggcaccag gatcgcatag
     1201 atcagcgaga tccgctccca cgccatgtgc atgaagatcg agacgatcag ccccgccttc
     1261 agcaccatga aggtcacgat caggaaccag cggagcccgc cctgcacgtg gaaataatcg
     1321 accagatacg acatcgtgct cagtacgaag aggtagcccc agaccttgag gtagaggccg
     1381 atcggatgct gttggccgcc ggcatggccc tcgctggcgg gcggggccgc ctgcatcggc
     1441 gcggggcttg gcgtgtggtt catgggctcc ccccctcacc agagatagaa gaacgcgaag
     1501 atgaacaccc agacgagatc gacgaagtgc cagtagagcc ctgcgatctc gaccgctgaa
     1561 tagtccgcgc ggcgatcata gtggccgcgc agcaccttgc tcgccacgat cgcgagcatg
     1621 atcacgcccg ccgagacgtg cagtccgtga aagccggtga tcatgaagaa ggaggcgccg
     1681 aattgcggtg cgcccatcgg gttggtccag gggcggacac cttcctcgaa gatcagcttg
     1741 tgccattcga aggcctgcat cgacacgaag ctcgcgccga gcagcgcggt cgcgcccatc
     1801 agtgcggcgg tgacgcggcg gttgcggcgg tagccgaagt tgaccgccat cgccatcgtg
     1861 ccgctgctgc tgatcagcac gaaggtcatg atcgcgatca ggattagggg gatggtctgc
     1921 ccgccgatcg tcagcgcgaa caccttgctg ggatcgggcc agggtagcgt cgccgacgcg
     1981 cgcatcgtca tgtaggaggt gaggaagcag gagaagatga aggtgtcgct gagcaggaag
     2041 atccacatca tggccttccc ccagtggaca tgccggaaca cctcctgatc ggccgaccag
     2101 tccgccgcga agccgccgag cccctgcggc tcagtgccgg ttgctgcggc gttggtctcg
     2161 atcgcttgcg ccattgctcg cgtccttcgg tcaggtcagc aggaacaggc cgaacatcca
     2221 caaccacacc agcagcagga agtgccaata gagtccgcac aggtcgacct gctggcgcac
     2281 cgccgcgatg gatccgccgc gcagcacctg gctggtcgcg tggccccaca ccgcgacccc
     2341 gccgagcaga tgcagcccgt gcaaggcggt gatgaggtag aagaaggcga tcgctgggct
     2401 accggtcagg aagggatgta atgtctggaa cgacctgtcg ggcgttccca agccgatcag
     2461 gcagaagccg ggctgggacg acaggaaata gccggcctcg cgcagctggt gccagacgaa
     2521 gagttggccg gagaggaagg caaggcccag cgcgcctgcg acgatcagcg cggatcggag
     2581 cgcggcggcg tcgccccgcc gagcagcgcg ccgcgccgcc tgccaggcga agctgctgag
     2641 caggaggatg ccgctgttcg cccagagcaa aggtggcttg ggcatcgagt gccagtgccc
     2701 gccggtctcg cccgacgcgg tgccatggcc cgacatgtgc atcagatagg ccatcgtcag
     2761 cagcaggaag agcatggtga cgacgcacag gaacaccgca aggccggtcg cgggcgtcga
     2821 tcgatccggg cgatcgcctg ccgggccggg cgccagccag ggcttttcgg taagggcggc
     2881 gagcaggctc atgtcgggtg gtgccgatcg gcgtcgcccg gcggcacgtt ctgggggatg
     2941 aagtcctgtg ccgcgccggg cacgctgtaa tcataggccc agcgatagac ggtggggagc
     3001 ctgggccccc aattgccgtg cttcggcggc gtatcgggcg tctgccactc aaggctcgct
     3061 gcaccccagg ggttgggatc ggccttgggg cctcggaaga agctccagat catgttgaag
     3121 aagaacagcc cctgggccac gaacacgatc aacgcggcga tggtgatccc ctcgttggcg
     3181 agatgcgccg acggcgggat gaaggcggtc gggccgagcg cgtaatagcg acggggcacg
     3241 cccaggatgc cgagataatg catcggcagg tagatcgcgt agacgccgag gaaggtgatc
     3301 cagaagtgga tcttgccgag catgtcgttc cacatccgcc cggtgatctt aggataccag
     3361 tgatagatcg cgccgaagat caccaggatc ggtgacaccc ccatcaccat gtgaaaatgg
     3421 ccgaccacga aaaaggtgtc ggacagcggc aggtcgacgc tgacattgcc gaggaacagc
     3481 cccgacaggc cgccatggat gaaggtgaac aggaagccga tcgcgaacag catcggcacg
     3541 cgcaggtgga tattgccgcg atagagcgtc aacagccagt tatagacctt gattgcggtc
     3601 gggatggcga tgatcagcgt cgatgtcgcg aagaagaacc cgaaataggg gttcatgccg
     3661 ctcacataca tatggtgtgc ccagacgaag aagctcagcg cgccgatgac gatgatcgcc
     3721 cataccatca tacgatatcc gaagatattc ttgcgcgcat ggacgctgat gagatcggag
     3781 acgatgccga aggcgggcag cgcgacgata tagacctcgg ggtggccgaa gaaccagaac
     3841 aggtgctgga acaggatcgg gcttccgccc tgataaccgg tctgctgtcc catcgagacg
     3901 atcgacggca tgaagaagct ggtcccggcg atccggtcga gcagcatcat gatcgcggcc
     3961 acgaacagcg ccggaaaggc gagcaggccc atgatgctgg cgacgaagat gccccacacc
     4021 gaacagggca tgcgcatcag cgtcatcccc ttggtgcgcg cctggagcac cgtcgtcaca
     4081 tagttgaggc cgcccatggt gaaggcgacg atgaagatcg cgaggctgac gagcatcgtg
     4141 atgatgcccc attggtggcc tggcgtgcca tcgaggatcg cctgcggcgg atagagcgtc
     4201 cagcccgacc ctgccggtcc gccgggcacg aagaaacccg acaccagaac cagcaccgag
     4261 agcagataga cccagtagct cagcatgttg acgaacggga acaccatgtc cctggcgccg
     4321 accatcagcg ggatgagata attgccgaac ccgcccagca gcagggccgt cagcagatac
     4381 accaccatga tcatgccgtg catggtgacg tactggagat aatgatcggg ctggatgaag
     4441 gaaaaggtgc cggggaagcc gagctgcagg cgcatcaacg ccgacagcac cagtgccacc
     4501 accccgatcg agatcgcggt cagcgaatat tggatggcga tgaccttatg gtcctggctc
     4561 cagatatatt tgccgatgaa ggtcttggga tgatgcagcg gccgcatgtc gtcggccaaa
     4621 gtgacggtcg ccatcagcgt ttccctctcg gttgatcggc ggcggtcggg atcgtcgtcg
     4681 tcttcgcctg ggcaagctcg gtgccggcag gcgcggacgc cgcagcgagc tgggcgtagg
     4741 tcggctgctt ggcgagccat gcgcgaaagg ccggttccgg ctcgacgatc acggtgccgc
     4801 gcatcatgta atggccgacg ccgcagagct cggcgcacag cacttcatag gtgccggggc
     4861 gtgtcggggt cagccagaaa taggtgatga tgccgggaac catgtccatc ctggcacgga
     4921 attcgggcac gtagaaatcg tgcagcacgt ccttagatct gagcagcatc ttgaccggct
     4981 tgttgagcgg gaggtgcagt tcgctgccct cgatcagccg gtcgtcgcgg ccataggggt
     5041 cctgcggatt gagcccgaac ggattgtcgg gcgtgatatt gcggacgtcg ctggtgccga
     5101 gcacgccatc ggggccgggg aagcggtagc tccattgcca ttgctgcccg atcgcctcga
     5161 cgatcgaggc atccttgggc acgttgacga agcggttcca ggcgagcagc cccggcgcga
     5221 gcattccggc gatcccgatc gcggtccaga aagtcagccg cttttcgagg cgcgtgtttt
     5281 ccggctcata gtgcgcgcga tggccgtcac gatagcgata gcggaacagc gcataggcga
     5341 ggaacaggtt gagcgctacg aaggcgaccg cgcagatcca gagcgtgatg ctcagcgcgc
     5401 tgtcgatcga accccagttc gacgcaagcg gggtccgcca ccaggggctc agcaggtgaa
     5461 aggcgatcga gcccagcacc aacagggcaa ttgcgactgc agcagccatc ctcatccccg
     5521 cgcatggctt ttgcgcctcg aagtgccagg aagagcccta ctttgctcct ccggcagacg
     5581 ggaagccgcc actcggaagc acactatata tcagctcacc ctccgtagaa agcgctctgt
     5641 cttcgtcgca ccatgtgcct ttcagtagat ctcggaacag gtagatcagg cgaatgcttg
     5701 cagacattgc gaagccgata gtcgcccgac cgcgaagagg accatgcttc cggcacaaag
     5761 acgcccgtca gatcgacgtt gggatcgaga accatggtga aacagcggcg ttcagctttc
     5821 agtgaagctg ttcattcccg ttgaggagtg gcgagcgggc ccagcgcgtg cctatagcca
     5881 ggaggaggaa gtggcctgca cctgatggcg gggctgacac ccggaggcgg tgggtgctgg
     5941 tcacgctgca atcgatccgc cgccagtggc cgaccgcatt gcgcctgctc gtcgagccca
     6001 ttcgggagtt gcgccctctc gggcttggat atctgccagt tccgggagca atgatgaatt
     6061 tcattcattg ctccatgcaa catcagcgac ctaatcctcc gagcggtcac gctctatctg
     6121 ctgtccgtcc ggcgcccgct ttccatcgat ccgccaccac caaccagcga ggaattccac
     6181 tcatggccac caacgcctat ggcgcgcatg ccgccgacac gccgctcgag cctatcatga
     6241 tcgaacgccg cgcaccgggg ccgcatgatg tccagatcga aatcgcctat tgcggcgtct
     6301 gccattcgga cctgcacacc gtccgctccg agtggggcgg caccgtctat ccgtgcgtgc
     6361 ccggccacga gatcgt
//
LOCUS       DATGEM010000116         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_995568, whole genome shotgun sequence.
ACCESSION   DATGEM010000116 DATGEM010000000
VERSION     DATGEM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995568"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="recA"
                     /locus_tag="VKQ27_03690"
     CDS             complement(<1..827)
                     /gene="recA"
                     /locus_tag="VKQ27_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLY88061.1"
                     /translation="MSAGAAALRLVEGSSMDKSKALDAALSQIERNFGKGSIMRLGKN
                     DKSMDIDVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLALHCLAEAQKKGGI
                     CAFIDAEHALDPIYARKLGVNVDDLLISQPDAGEQALEIADTLVRSGAVDVLVIDSVA
                     ALVPRAELEGEMGDSQPGLQARLMSQALRKLTASINRSNTMVIFINQIRMKIGVMYGS
                     PETTTGGHALKFYASVRLDIRRIGAIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDI"
     gene            complement(1401..>2113)
                     /locus_tag="VKQ27_03695"
     CDS             complement(1401..>2113)
                     /locus_tag="VKQ27_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HLY88062.1"
                     /translation="FVGKKQRHLKRTDSPEKGDQYTFVALASSSRAIIGYRTGKRNGD
                     ATDQFIQDLRGRVIGSPEISTDGFLPYQPAIRDAFQASAHGVINKTFSVTHLVHMDQA
                     HHRYSPAAVIAVAREAVRGEPEQISTSYVERSNLSIRMANRRFTRLTNAFSKKLDNHL
                     SAVALYVAHYNLCRVHESLSPSARNQVTPAMALGIADRPWSLGELIDAALAVATPDPT
                     ETAPERRRKFRVIEGGRQ"
BASE COUNT          376 a          645 c          658 g          434 t
ORIGIN      
        1 atgatgtcga actcgacctg cttgaacggc ggagcgagct tgttcttgac cactttgacg
       61 cgggtctggt tgccgaccac ctcgtcgcgt tccttgatgg cgccgatgcg gcggatgtcg
      121 agacgcaccg aggcatagaa cttgagcgca tggccgccgg tcgtggtctc cgggctgcca
      181 tacatcacgc cgatcttcat gcggatctgg ttgatgaaga tcaccatcgt gttggagcgg
      241 ttgatcgagg cggtgagctt gcgcagcgct tggctcatca accgcgcctg caggccgggc
      301 tggctgtcgc ccatctcgcc ttcgagctcg gcgcgcggca ccaaggccgc caccgaatcg
      361 atgaccagca cgtcgaccgc gccggagcgc accaaggtgt cggcgatttc cagcgcctgc
      421 tcgccggcat cgggctgcga gatcaacagg tcgtcgacat tgacgccgag tttgcgcgca
      481 tagatcggat cgagcgcgtg ctcggcgtcg atgaaggcgc agatgccgcc cttcttctgc
      541 gcctcggcca ggcaatgcaa agcgagcgtc gtcttgcccg acgattcggg gccgtaaatc
      601 tcgaccaccc ggccgcgcgg caggccgccg acgccgagcg cgatgtcgag cccgagcgag
      661 cccgtcgaca ccacgtcgat gtccatcgac ttgtcgttct tgccaagccg catgatcgag
      721 cccttgccga aattgcgctc gatctgcgag agcgcggcat caagggcctt cgacttatcc
      781 atggacgaac cttcgacgag acgtagcgct gctgcacctg cactcatgat tccgtgctcc
      841 ttatggccgc taatctgtga gccgacaacg gacgccaagc cgcgcgcaaa acgttgcgaa
      901 cgccagcccc cgaagccggg ccccgcaaag ggcgatgggg agcaatgtac atgatttgtt
      961 ctcggttcgc aatatgttct tttggccctt tgggttctgt cctaactccg gtggattagg
     1021 acactccatt ccctcagtta ggacactact gcggtaaaat tcgccgtcac tggcgattcc
     1081 tttctgctga ccagcggggc ctctgcgtcc cgaggccatt cgcggggtgt gctcccgcaa
     1141 agcagaaagg aggcttccat gagtaggcat ggggccaaac ctaaatctgt gtatgtccgc
     1201 gcgtacactc gttggcgtta cgggcgtaat gagaatgtgc gttctcattt ccgctcgtgg
     1261 cccggacaac tcgacttcgg tttttgacgt gtaggcacgg cctaacgtgc cagtgacggc
     1321 gttgccatga tgagaaggcg gctgctctcc cgtcatggcg gtggactggc gaaacccggc
     1381 tccgttgccg ggtttcgttt ttactgacgg cctccttcga tcacgcgaaa cttgcgacgc
     1441 cgctccgggg cagtttcggt cggatcggga gtggcaaccg ctagcgccgc gtcgatcaac
     1501 tcgccaagcg accacggccg atccgcgatg cccaacgcca tcgctggcgt cacttggttg
     1561 cgagcgctcg gcgaaagcga ctcgtgaacg cggcacagat tgtaatgcgc cacatacagg
     1621 gcgaccgccg acagatgatt gtccagcttc ttgctgaatg cgttggtcag ccgggtaaag
     1681 cgtcggttcg ccatgcggat cgaaaggttg ctgcgctcca cataggacgt gctgatttgc
     1741 tccggctcgc cgcgcaccgc ttccctcgca accgcgataa ccgccgctgg cgagtagcga
     1801 tgatgcgctt ggtccatatg gactaagtga gtgacgctga aagtcttgtt gatgacgcca
     1861 tgggcgctcg cctggaacgc atcgcggatg gcgggctggt agggcaagaa gccatccgtt
     1921 gagatttccg gcgagccgat cacccggccg cgcaagtcct gaatgaattg gtccgtagcg
     1981 tcgccattcc gcttgccggt gcggtagccg atgatggcgc gggacgatga ggccaaggca
     2041 acgaacgtgt attggtcgcc cttctcgggg ctgtcggtgc gctttagatg ccgctgcttc
     2101 ttgccaacga acg
//
LOCUS       DATGEM010000117         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_995428, whole genome shotgun sequence.
ACCESSION   DATGEM010000117 DATGEM010000000
VERSION     DATGEM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995428"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1205)
                     /locus_tag="VKQ27_03700"
     CDS             complement(12..1205)
                     /locus_tag="VKQ27_03700"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY88063.1"
                     /translation="MTEPTGPLKGIRILDLTTVLFGPFGTQTLGDWGAEIIKVESLTG
                     DTWRTSGVFRNRGMSGQFMAANRNKRSIALDLKHPDGKEVLRRLIPTVDALVSNIRPA
                     GLARLGFSYEACRELNPTIVYATAVGFGQDGPWRARPAFDEIIQAASGFASAMGTDDE
                     PAFVPSLIGDKICGMALTAAVTAALLHCARTGEGQMVEVPMLETLAAFNSIEMFGGIA
                     FVPPVGPAGYKRMKARRPVRTKDGWLTMLPYSGDNWCAFFAAVGHPECIEEFSVRDPV
                     ARARNIDRIYDRMRDIAVTRTTREWEELLLSIDVPHTSFARLSEVAEQPHLKAVGLFQ
                     EVQHPTEGTLRQARPPARFSASPAGVRRLAPRLGEHTREVLREAGYGDAEIEGLVEKK
                     AVGVG"
     gene            complement(1258..>2161)
                     /locus_tag="VKQ27_03705"
     CDS             complement(1258..>2161)
                     /locus_tag="VKQ27_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY88064.1"
                     /translation="SLNAGRLPAAVGRELAGSPADYRCAGSARVGGGAWEVDGGYRVK
                     GRWNFASGIDNANWLYCTCTMMDGDVPRRNAAGAPVLRAVWVPREQVTIVDTWSVMGM
                     RGTGSQDFTVDDMFVPVRRSVLLDDAPAERGPLFNQRAWYVVLWTPSAANALGIARGA
                     IDSLAELAETEASTLSTALLRDRPMVQARVGEAEAIVNAARAYVFDAVGRLWDTLCVG
                     GTPSDRLVMQARLALVHAMHESVRAVDKVFHAAGTNAIYTRHPLERAFRDVHVALQHG
                     AALPSYFESAGKVLLGLRPGDPGW"
BASE COUNT          379 a          890 c          555 g          337 t
ORIGIN      
        1 acatcatctt gctaccccac ccccacagcc ttcttctcca ccagcccctc gatctccgca
       61 tccccgtatc ccgcctcccg cagcacctcg cgcgtatgct cccccagcct gggcgccagt
      121 cgccgcaccc ccgccgggct ggcggaaaac cgcgccggag gccgcgcctg ccgtagagtc
      181 ccctcggtcg gatgctgcac ctcctggaac agccccaccg ccttcaggtg cggctgctcc
      241 gccacctcgc tcagcctggc aaacgacgtg tgcggcacat cgatcgacag cagcagctcc
      301 tcccactccc gtgtcgtccg cgtcaccgcg atatcgcgca tacggtcgta gattcgatcg
      361 atattccgcg cccgcgccac cgggtcgcgc acggaaaact cctcgatgca ctccggatgc
      421 cccaccgccg cgaagaacgc gcaccagttg tcgcccgagt aaggcagcat cgtcagccac
      481 ccgtccttcg tccgcaccgg ccgccgcgcc ttcatccgct tgtagcccgc gggccccacc
      541 ggcggcacga acgcaattcc tccgaacatc tcgatgctgt tgaacgccgc caacgtctcc
      601 agcatcggca cctccaccat ctgcccttca cccgtccgag cgcaatgcag caacgccgcc
      661 gtcaccgcgg cggtcagtgc catcccgcag atcttgtcac cgatcaggct gggcacgaac
      721 gccggctcgt catccgtccc catcgccgag gcaaatcccg acgccgcctg gatgatctca
      781 tcgaacgccg gccgcgcgcg ccacggcccg tcctgcccga agcccaccgc cgtcgcatag
      841 acgatcgtcg gattcagctc ccggcacgcc tcatagctga accccagccg ggccagcccc
      901 gccggccgga tattgcttac cagcgcatcg accgtcggga tcagccgccg cagcacctcc
      961 ttcccatcgg gatgcttcag atccagcgcg atgctccgct tgttgcggtt cgccgccatg
     1021 aactgcccgc tcatgccacg attgcggaac acgcccgagg tccgccaggt gtcccctgtc
     1081 aggctctcca ccttgatgat ctcggcgccc cagtcgccca gcgtctgcgt gccgaacggc
     1141 ccgaacagca ccgtcgtcag gtcaaggatg cggatcccct tcagtggtcc ggtcggctcg
     1201 gtcatcttgg ttcggtccct cgctagcgtc ccgcgacaat gccgccaagc gccccaatca
     1261 ccaacccgga tcgcccggcc gcaaccccag caggaccttg cccgcggact cgaaatagct
     1321 gggcagcgcc gcgccgtgct gcaacgccac gtgcacatcg cggaatgcgc gctccagcgg
     1381 atggcgcgta tagatcgcgt tggttcccgc cgcatggaac accttgtcca ccgcccgcac
     1441 cgactcatgc atggcatgca cgagagccaa ccgtgcctgc atcaccaggc gatcggacgg
     1501 cgtcccgccc acgcacagcg tatcccacaa ccgccccacc gcatcgaaca cataagcacg
     1561 cgcggcattc acgatcgcct ccgcctcgcc gacccgcgcc tgcaccattg gtcgatcgcg
     1621 cagcaatgcg gtggacagcg tggacgcctc cgtctccgcc aattcagcca aactatcgat
     1681 cgccccgcgc gcaatcccca gcgcattcgc cgccgaggga gtccacagca ccacatacca
     1741 ggcgcgctga ttgaacaatg gcccgcgctc tgcaggcgca tcatccaaaa gcacgctgcg
     1801 ccgcaccggt acgaacatat cgtcgacggt gaaatcctgg cttccggtgc cgcgcattcc
     1861 catcaccgac cacgtatcca caatcgtcac ctgctcgcgc ggtacccaca ccgcacgcag
     1921 caccggcgcg cccgctgcat tccgtcgcgg cacatcgcca tccatcatcg tgcaggtgca
     1981 gtacagccag tttgcgttgt cgatcccgct ggcgaaattc caccgtccct tcacgcgata
     2041 tccgccatcc acctcccatg cgccgccgcc cactctagct gaccctgcgc agcgataatc
     2101 cgcgggagac ccagccagct cacgccccac cgcagccggt aaccgccccg cattcagcga
     2161 c
//
LOCUS       DATGEM010000118         7466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1335632, whole genome shotgun sequence.
ACCESSION   DATGEM010000118 DATGEM010000000
VERSION     DATGEM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335632"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VKQ27_03710"
     CDS             complement(<1..386)
                     /locus_tag="VKQ27_03710"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY88065.1"
                     /translation="MNTIVRKLSFLAVLLTFIPNIAGAAVDDEINRMEESRYAALIGK
                     DWKALDAILADDFFYNTAGGAQMSKSEFIGLMQSGTAVVRKAVRENATVRPYGDVTLV
                     TGIAHIDVTLKGEDKTLHSRYLHVWV"
     gene            complement(952..2181)
                     /locus_tag="VKQ27_03715"
     CDS             complement(952..2181)
                     /locus_tag="VKQ27_03715"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY88066.1"
                     /translation="MTPVAPADADEGLLGACTVIDFTRVLAGPYCTRLLADLGARVIK
                     IERPGEGDEIRYTAFQLDPGRTDQSSYYARLNTGKQGIAIDLSHPQARQIVFDLVRIA
                     DVVVENFSPGVMARYGLDAAALRREFPGLVYCSISGFGQTGPLSSMQAYAHLINAISG
                     MMDLDRAGEAQPRVSYLQAADVLAGTHAFGAICAALLRRARKGQGATLDISMLECLIA
                     ADDITYGALLNGGRVERQPRIGMMVHAFGERHIALQTAGAPHLWSRLVALVGQPELAT
                     DPRFSTPQARRDNWSALQQVYRGWLSRYQTADEAIAALSAARIPVVPMLSPEEVVEHP
                     QLVAREAFPEVAHPGCGSIRVTATPFFVDGKPTHPRASAPYRIGEHTHDVLSNMLGYP
                     GDRIETLRKLGVIEMPD"
     gene            complement(2327..3178)
                     /locus_tag="VKQ27_03720"
     CDS             complement(2327..3178)
                     /locus_tag="VKQ27_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY88067.1"
                     /translation="MDKPAGRNYRHIEVQPIAGALGADILGVDIARPLEAEVVSEIHR
                     AFLDHLVVFFRDQKLTPQAQLAFSRQFGVPMEYPQLRGLPECPLIVPVVKLEHEVQNF
                     GGLWHSDTTYLEHPPMGSMLYAVETPPFGGDTLFANQYLAYASLSEGLKRILGELTGV
                     NTSTKANATKTREDRLRSDGLELKALLARHPVVRTHPETKRKALYVNVGHTTQFDGWT
                     EEESRPLLDFLFAHQVRPEFTCRCRWEPGTLAFWDNRCTQHNPVNDYQGYRRVMHRVT
                     LAGDAPC"
     gene            complement(3465..4601)
                     /locus_tag="VKQ27_03725"
     CDS             complement(3465..4601)
                     /locus_tag="VKQ27_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88068.1"
                     /translation="MNLRHWVASIYYLGIKELFSLRYDTVLLILIVYSFTYAVLGPAK
                     GAKMELINASVALVDEDRSALSSRIRDALQKPYFLPARQLSLGEIDDAMDADRYTFAI
                     DIPPDFQADALAGRKPTIQVNVDATAMSQAGRGAGYIQSVITQETATFLHIREADLLP
                     RVALVTRAKFNPNLMSPWFVAVMQLVNNITMLAIILSGAALVREREHGTIEHLLVMPL
                     TPAQIMLAKVWANGLVIVVVATVSLRLVVQWLLDVPVAGSLPLFMFSTFVYMFSVTSL
                     GIFLATIARSMPQFGLLAFPVFIVMNLLSGGTTPLDSMPDFLQKVMLVSPTTHFVNIA
                     QAILYRGAGLDAVWPSLAAVAAIGVVFFVAALMRFRRTVTAMQA"
     gene            complement(4598..7339)
                     /gene="rbbA"
                     /locus_tag="VKQ27_03730"
     CDS             complement(4598..7339)
                     /gene="rbbA"
                     /locus_tag="VKQ27_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HLY88069.1"
                     /translation="MQSSALVARLSAIGLRYGSTVALDDVNLDIPAGCIVGFIGPDGV
                     GKSSLLALLSSARKIQSGSVEVLGGNMADVRHRNAVCPRIAYMPQGLGKNLYPTLSVA
                     ENLEFFARLFGQSRAERAWRMQELLHSTGLAPFAERSVEKLSGGMKQKLGLCCALIHD
                     PDLMILDEPTTGVDPLSRRQFWELIARIRSRRPGMSVLVATAYMDEAERFDWLIAMDA
                     GKVLAAGTPQSIQSRMHAGNLEEAFVALLPADKRAGHQRLAIPPRRSQDDTPAIEARD
                     LTRQFGDFVAVDHVSFQIGRGEIFGFVGSNGCGKTTTMKMLTGLLPPSAGQALLFGAP
                     MDADGLATRSKVGFMSQSFSLYTELTVRQNLVLHARLFHLPRAQVAARVTQMTERFGL
                     VQYMDQLTEKLPLGVRQRLALAVAVIHEPEMLILDEPTSGVDPVARDRFWELLIDLSR
                     NRGVTIFVSTHFMYEAQRCDRVALMHAGKVLAQGTPAAITQERGAANLEEAFIAYLED
                     AESGEQGAAHGTPAATAFQAPVRAAQDPAPVRFSSGRLWAYVRRETLEVLRDPIRLAF
                     ALLGPIMLMIVFGYGISFDVENLSYAALDRDRSPESRTYLENFSSSRYFREQPALQDH
                     MDMEQGLRSGRLKFAIEIPPDFGKDLKRGRHPEVSAVLDGANPFRAETSRGYLEGVHQ
                     AYLARIARDDGQPPPALPVNVEIRFRYNQDFKSVFAMVPGVIMMVLILIPAMMTALGV
                     VREKELGSITNLYVTPVTRLEFLLGKQLPYVVLSLVNFACLLLLTVFLFEVPVKGSLP
                     ALVGGAMLYVIAATEFGLLISTFVKSQIAAIFASAILSTLPAIQFSGLLLPVSSLSRD
                     AKIMGAAFPSTYFHHISVGVITKGLRLHDLIPDYAALIAVIGAYLLIALALLRKQER"
BASE COUNT         1383 a         2333 c         2440 g         1310 t
ORIGIN      
        1 ttcacccaga cgtgcaggta gcgggaatgg agggttttgt cttccccttt cagcgtcacg
       61 tcgatgtggg cgatgccggt cacaagcgtc acgtctccgt atgggcggac ggttgcgttt
      121 tcccggacgg ccttcctgac cacggcggtg ccggattgca tcaggccgat gaactcgctc
      181 ttgctcatct gcgcgccgcc ggcggtgttg tagaaaaagt cgtcggcgag tatggcgtcg
      241 agggccttcc agtccttgcc aatcaacgcg gcgtagcggc tttcctccat gcgattgatc
      301 tcatcatcga cggcagcgcc ggcgatattc gggatgaagg tcaagagaac cgccagaaac
      361 gacaacttgc gcacgatcgt gttcatgtca tcctccttcg tatcgattgt tgaagattac
      421 gtcccttggt gccgccgtta ttatcaggct tgtgcaattg cctgcgacaa cattgcgcgt
      481 cgaacgacaa gccggtagag gtaacgcagt gattggtgaa ttgcgaagtg cggttttgac
      541 cttgggttcc ccttgagtcg cgccgagcag ctcgactcga caagggggtg tcggcgagga
      601 ctgttcgagt cccatggcag ggcacgttgt gtgtgccctg ccaggtcgag ttccgcagcc
      661 gccttgtcga gtcaggcagc acaggggcac cccgcgaatg cggggcgcga ctccaggggc
      721 ggccttcttt cggttacctt tcttggccgc gcaagaaagg tgaccagccg ccgggctgcc
      781 cccggcctgg tttatccgtc agttgacgca gcttacggtc gcagtccagg ccgcacaggt
      841 taaagaattt cgaatccgcc gcaacgaacg cccgagtgca aactctcgcc cgacagtgtg
      901 ccttcgcggc tcgcgcagcg tcatatcaac ggctccaatc acgaggcaac gtcaatccgg
      961 catttcgatg acgccgagtt ttcgcaaggt ttctattctg tcgccaggat agcccagcat
     1021 gttgctgagc acatcgtgcg tgtgctcgcc gatccggtat ggcgcgcttg cgcgcggatg
     1081 cgtgggcttg ccatcgacaa aaaagggagt ggcggtgacg cggatgctgc cgcagccggg
     1141 atgggccact tccggaaacg cctcgcgcgc gacgagctgc ggatgctcga caacctcttc
     1201 cggcgacagc atcggcacca ccggtatgcg ggcggccgac aacgctgcga ttgcctcgtc
     1261 tgcggtctgg tagcggctga gccagccgcg ataaacctgt tgcagcgcag accagttatc
     1321 gcgtcgcgcc tgcggcgtcg agaaacgcgg gtcggttgcc agttccggtt ggccgaccag
     1381 cgcgaccagc ctcgaccaga ggtgcggcgc acccgccgtc tgcagcgcga tgtggcgctc
     1441 gccgaacgcg tgcaccatca tgccgatgcg cggctggcgt tcgacccgcc cgccattcag
     1501 cagcgcgccg taagtaatgt cgtccgcggc aatcaggcac tcgagcatgg aaatatcgag
     1561 cgtcgcaccc tgacccttgc gggcgcgccg cagcagcgcg gcacaaatgg cgccgaaggc
     1621 atgcgtgccg gcgaggacgt cggcggcttg cagataggaa acgcgcggtt gtgcctctcc
     1681 cgcacgatcc aaatccatca tgccggagat cgcattgatc agatgcgcgt aggcctgcat
     1741 ggaactgagc gggccggtct ggccaaaacc ggatatcgag caatacacca gtcccggaaa
     1801 ttcgcggcgc agcgcagccg cgtccaggcc atagcgtgcc atcacacccg gcgagaaatt
     1861 ctccacgacg acgtcggcga tccgcaccag atcgaagacg atctgccggg cttgcgggtg
     1921 cgagaggtcg atcgcaatgc cctgcttgcc ggtgttcagg cgcgcgtaat agctgctttg
     1981 gtcggtgcgg ccggggtcga gctggaaggc ggtgtaacga atctcgtcgc cctcgcccgg
     2041 ccgttcgatc ttgatgaccc gcgcgccgag atcggcaagc aggcgcgtgc aataaggacc
     2101 ggcgaggacg cgggtaaagt cgatgacagt gcaggcaccg agcaggcctt cgtcggcatc
     2161 ggcgggggca acaggggtca cgatatcggg cggcgttgat aaggttgaac gatgtcgacg
     2221 gcaggcaggc acgcacatct gcccggcctg actgcccggc cctgccagga ccccgtcgcg
     2281 atcgggcgat tttatccgct atcggcccct ggagatacac gaagactcaa cagggcgcgt
     2341 ccccggcgag ggttacgcga tgcatcacgc gccggtagcc ctgatagtca ttcaccgggt
     2401 tgtgctgcgt gcagcgattg tcccagaacg ccagcgtgcc cggctcccac cggcagcggc
     2461 aggtaaattc cggcctgacc tgatgcgcga ataggaaatc gagcaacggc cggctctcct
     2521 cctccgtcca accgtcaaac tgcgtggtgt ggccgacatt gacgtagagg gccttgcgct
     2581 tcgtctccgg gtgggtgcgc acgaccgggt gccgggcgag cagcgccttg agctcaagcc
     2641 catcgctgcg caagcggtct tcgcgagttt tcgtcgcgtt ggctttcgtc gaggtattga
     2701 cgccggtgag ctcaccaagg atccgcttga ggccttcaga cagggatgcg taggccaggt
     2761 attgattcgc gaacagggtg tcgccgccaa atgggggagt ctcgacggcg tagagcatgc
     2821 tccccatcgg cgggtgctcg agataggtgg tgtccgaatg ccacaggccg ccgaagttct
     2881 gcacttcgtg ctccagcttg accaccggca caatgagcgg gcattcgggc aatccccgca
     2941 actgcggata ttccatcggc acgccaaact gcctggagaa ggcgagctgc gcttgcgggg
     3001 tgagcttctg atcgcggaag aaaacgacga ggtgatcgag gaaagcccgg tggatctccg
     3061 acacgacttc cgcctcgagg ggccgcgcga tatccactcc caggatatcg gcccccagtg
     3121 cgccggcgat cggctgcacc tcgatatgac ggtagttccg gcccgccggc ttgtccattg
     3181 gagttctccg cttcctggca tcgtgcattc aggtgaggcc gcgtgtgcct ctcccccggc
     3241 ctgcgactat tgtggcgggc tgcaatctcg cgtctcatac tctagatcgc ttctgcaaac
     3301 tccaccagta gcgtcgcaat acatctttcg cggattacga aataattcac ccgacacttt
     3361 cgcttcgcgg ctctgctgcg gcggtatccg gaaggtggcc tggcagccat tagcggccat
     3421 cgcggaagac acgacggcct tctccgcccg cgcccgaccc cgcttcatgc ctgcatcgcg
     3481 gtgacggtcc tgcgaaagcg catcagtgcc gcgacgaaaa acacgacccc gattgccgcg
     3541 accgccgcaa ggctcggcca gaccgcatcg agccccgcgc cgcgatagag aatcgcctgg
     3601 gcgatgttga cgaagtgcgt cgtcggcgag accagcatca ctttctgcag gaagtccggc
     3661 atgctgtcca ggggcgtggt tccgccggac agaaggttca tgacaatgaa caccgggaac
     3721 gccagcagtc cgaactgcgg catcgagcgg gcgatggtgg cgaggaagat gccgagcgag
     3781 gtgaccgaga acatgtagac gaacgtgctg aacatgaaca acggcaacga tcccgccacg
     3841 ggcacgtcca gaagccattg gaccacgagg cgcaacgaca ccgtggcgac aacgacgatg
     3901 accagaccgt tggcccacac cttcgcaagc atgatctggg ctggcgtgag gggcatgacc
     3961 agcaggtgct cgatagtgcc gtgctcgcgc tcgcgcacca gagccgcccc ggacaggatg
     4021 atcgccagca tggtgatgtt gttcaccaac tgcatcacgg cgacgaacca gggcgacatg
     4081 aggttggggt tgaacttcgc tcgcgtcacc agcgccaccc ggggcaagag gtcggcttcg
     4141 cggatgtgca ggaaggtggc ggtttcctgc gtgatcacac tctgaatgta gcccgcgccg
     4201 cgccccgcct gactcatcgc cgtggcatcc acgttgacct ggatggtggg ctttcgccct
     4261 gccagcgcgt cggcctggaa gtccggcgga atgtcgatgg cgaaagtgta gcggtccgcg
     4321 tccatcgcgt cgtcgatctc gcccagggac agctgcctgg ccggaaggaa gtagggtttc
     4381 tgcagcgcat cgcgtatgcg gctggaaagg gccgagcggt cctcgtcgac cagggccacc
     4441 gaagcattga tcagttccat cttggcgccc ttggcgggac cgagcacggc ataggtaaac
     4501 gagtagacga tcaggatcag cagcacggtg tcgtagcgca ggctgaacag ctccttgatg
     4561 cccaggtaat agatgctcgc gacccagtgc ctgaggttca tcgctcttgc ttcctgagca
     4621 gggccagcgc gatcagcagg tacgcgccga tcactgcgat gagcgccgca tagtcgggga
     4681 tcaggtcgtg caagcgcagg cccttggtga tcacgccgac gctgatgtga tgaaaatagg
     4741 tgctggggaa tgcggcgccc atgatcttcg cgtcccgcga cagagacgac accggcaaca
     4801 gcagtccgga gaactggatg gccggcagcg tactgaggat ggccgaggcg aagatcgccg
     4861 cgatctggga tttcacgaag gtcgaaatca gcaggccgaa ctcggtggcg gcgatgacat
     4921 agagcattgc gcccccgacg agggcaggca ggctgccttt gaccggcacc tcgaacagga
     4981 acacggtcag cagcagcagg caggcgaagt tcaccagcga caacaccacg tagggcagct
     5041 gcttgccgag caaaaactcc agccgggtga cgggcgtgac atagagattg gtgatggagc
     5101 ccagttcctt ttcacgcacg acacccaacg ccgtcatcat cgccgggatc aggatcagca
     5161 ccatcataat gacgcccggg accattgcga acacgctctt gaagtcctgg ttgtagcgga
     5221 agcggatctc gacattcacg ggcagcgcgg gcggcggctg gccatcgtcc cgcgcgatgc
     5281 gggcgagata ggcctggtgg acgccctcga ggtagccgcg gctcgtttcc gcgcgaaacg
     5341 ggttggcgcc gtccagcacc gccgagactt ccggatggcg tccgcgcttg aggtccttgc
     5401 cgaagtcggg cgggatctcg atggcgaact tgaggcggcc gctgcgcagg ccttgttcca
     5461 tgtccatgtg atcctgcagc gcaggctgct cgcggaaata acgcgaggag gagaagttct
     5521 ccaggtaagt gcggctttcc ggggagcggt cccggtccag cgcggcgtag gacagattct
     5581 ccacatcgaa cgagatgccg tagccgaaca cgatcatgag catgatcggc ccgagcagcg
     5641 cgaacgccag ccggatcgga tcgcgcagga cctccagcgt ttcgcgtcgc acataggccc
     5701 acaggcgccc ggagctgaaa cgcacgggcg cgggatcctg cgccgcgcgc accggcgcct
     5761 gaaaagcagt ggcggcgggc gtgccgtgcg ccgcgccctg ctcgccggac tcggcgtcct
     5821 caaggtaggc gatgaaggcc tcttccaggt tcgcggcgcc gcgttcctgg gtgatcgcgg
     5881 ccggcgttcc ctgcgcgagc accttgccgg cgtgcatgag cgccacacgg tcgcagcgtt
     5941 gtgcctcgta catgaaatgc gtcgacacga aaatcgtcac gccgcggttg cgcgacaggt
     6001 cgatcagcag ctcccagaag cggtcgcgcg cgaccggatc cacgcccgag gtgggctcgt
     6061 cgaggatgag catctccggc tcgtggatga cggccaccgc gagcgccagg cgctggcgca
     6121 cgcccagggg cagcttttcc gtgagctgat ccatgtattg caccaacccg aatcgctcgg
     6181 tcatttgcgt gacgcgcgcc gcgacctgtg cgcggggcag atgaaacagc cgcgcgtgca
     6241 gcaccaggtt ctggcgcacg gtcagctcgg tgtagaggga aaaggattgg gacatgaagc
     6301 ccactttgct gcgcgtggcc agcccgtccg catccatggg tgcgccgaac agcagcgcct
     6361 gccccgccga aggcggcagc aagccggtga gcatcttcat cgtcgtggtc ttgccgcagc
     6421 cgttggagcc cacgaagccg aagatctcgc cacgcccgat ctggaaactc acgtggtcca
     6481 cggcgacgaa atcgccgaat tgcctggtga ggtcgcgggc ttcgatggcg ggcgtgtcgt
     6541 cctgggaccg gcgcgggggg atcgccaggc gctggtgtcc ggcgcgtttg tcggctggca
     6601 gcagggcgac gaaggcttcc tccagattgc cggcatgcat gcgcgactgg atgctctgcg
     6661 gcgtgccagc ggccagtacc tttcccgcgt ccatggcgat cagccagtcg aagcgctccg
     6721 cttcgtccat gtacgctgtg gccacgagta cgctcatgcc cggacggcgg ctgcgaatgc
     6781 gcgcgatcaa ctcccagaac tgtcgtcgcg acagcgggtc cacgccggtg gtcggctcat
     6841 ccaggatcat gagatccgga tcgtggatca gcgcgcagca cagcccaagc ttctgcttca
     6901 tgccgcccga aagtttttcc accgagcgct ccgcgaaggg ggccaggccg gtgctgtgca
     6961 gaagctcctg catgcgccac gcgcgctcgg cgcgcgattg accgaacagg cgcgcgaaaa
     7021 attccaggtt ctccgccacc gacagcgtcg ggtacaggtt cttgcccagc ccctggggca
     7081 tgtaggcgat gcgtgggcat acggcgttgc ggtgacgcac gtcggccata ttgccgccga
     7141 gcacttccac gctcccgctc tggatcttgc gcgcactgga gagcagtgcc agcaggctgg
     7201 acttgcccac accgtccggc ccgatgaagc cgacaatgca cccggcgggg atgtcaagat
     7261 tgacgtcgtc gagagccacg gtactgccgt accgcagtcc gattgcggac agccgcgcta
     7321 ccaaggctga cgattgcatc actgcggcag cttgacctgc agcgtgacgg gccagtctgc
     7381 cttgacatcg aggcgcacat aggccacccc gggaagcccg gtcttgacct gcggctcgta
     7441 tttcctgagc agctccggat cgatct
//
LOCUS       DATGEM010000119        14615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1240867, whole genome shotgun sequence.
ACCESSION   DATGEM010000119 DATGEM010000000
VERSION     DATGEM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14615
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240867"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..752)
                     /locus_tag="VKQ27_03735"
     CDS             complement(285..752)
                     /locus_tag="VKQ27_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLY88070.1"
                     /translation="MTTSAEGRDLVLTRLIAAPPEKLYRAWTDPALLKQWFAPLPYTT
                     PEAELDVRPGGSNLVVMRGPDGGDIPCHGVYLEVVPNRRLVVTDAFVSAWEPSQKPFM
                     TLVLTFEPEGEGTRYTARVRHWSVEDREAHEKMGFHQGWSLCADQLEALAGRI"
     gene            complement(798..2018)
                     /locus_tag="VKQ27_03740"
     CDS             complement(798..2018)
                     /locus_tag="VKQ27_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLY88071.1"
                     /translation="MTDLTWINGALTAARPQAIGALLRYFRDLDTAEEAYQEACLRAL
                     QNWPRNGPPRDPAAWLILVGRNAALDGARRRARQDPLPEEETLSDLEDVETPLAERLD
                     GSHYRDDVLRLLFICCHPELPATQQIALALRIVSGLTVAQIARAFLVSDAAMEQRITR
                     AKSRIARADVPFETPGAVERAERLAAVAAMIYLVFNEGYSARTSLCDEAIRLARLLLR
                     LFPAEPEVMGLTALMLLQHARAAARFDAAGEIVLLDDQDRSLWDGKLIAEGLALIDKA
                     MRHRAPGVYQVQAAIAALHARAARPEETDWAQIDALYAALERLQPSPVVTLNRAVAVS
                     KVQGAEAALAMIEPLAERLAGYFHFFGVKGGLLLQLGRGEEARVAFDRAIALANTPAE
                     AAHIRMHLDRLGVG"
     gene            complement(2015..2386)
                     /locus_tag="VKQ27_03745"
     CDS             complement(2015..2386)
                     /locus_tag="VKQ27_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLY88072.1"
                     /translation="MMYAVLCYNDEDVVWSWSKEEDAAVMARLGVVQQKLAREGKLGP
                     SLRLLPTTSATTLRKTQDPPLVIDGPFAETKEQLLGFYILDVANLDEALDAARELAAV
                     NPGGSYEIRPVALYHADPRAG"
     gene            complement(2488..2634)
                     /locus_tag="VKQ27_03750"
     CDS             complement(2488..2634)
                     /locus_tag="VKQ27_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88073.1"
                     /translation="MRAAQYRQLAAGIASVDVRNTLLQLAMRHESLAAGESGAEGAVG
                     RIAD"
     gene            complement(2790..3323)
                     /locus_tag="VKQ27_03755"
     CDS             complement(2790..3323)
                     /locus_tag="VKQ27_03755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY88074.1"
                     /translation="MNAMGVSVRQLTAEEAYGCNAALCDVLIDCVHGGASVSFMAPLC
                     DVKAQDFWRGVADGVARGDRVLLVAEDEAHGIIGTVQVVFAQPENQPHRADIAKMLVH
                     RRARRRGVGGLLMRAGEAVALAAGKTVLVLDTATGSDAERLYQRMGWNRVGEVPDYAL
                     WPDGRLCSTTYYYKAIG"
     gene            complement(3320..3910)
                     /locus_tag="VKQ27_03760"
     CDS             complement(3320..3910)
                     /locus_tag="VKQ27_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLY88075.1"
                     /translation="MEPSADIERRVADRLKALRAERGLTLDELAKRSGVSRSMISLIE
                     RAESSPTASVLDRLSAALGVTLASMFAEEARVDAQPVARRGEQTAWSDPETGYVRRNL
                     SPAGFPSPIELVEVVLPGGARVAYDSGFREPPVDQQVWVIEGAIEVRVGEQVHQLEAG
                     DCLAMRVDQPIGFRNRGRTAARHLVALINPQQRNFR"
     gene            4361..4597
                     /locus_tag="VKQ27_03765"
     CDS             4361..4597
                     /locus_tag="VKQ27_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88076.1"
                     /translation="MLDPAIRIVTLTVTGKGYHGPLPSLLAPAPQHRRAANIANHVVV
                     FKQAAIIEQGPPRPIFEAPLERTRDFLSHLGWEG"
     gene            4594..5652
                     /locus_tag="VKQ27_03770"
     CDS             4594..5652
                     /locus_tag="VKQ27_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY88077.1"
                     /translation="MNFFQPYSTESDRHSHLQSRRSGVHHASPLAKVRSGRATSYGSQ
                     AQAEGKRTVASFQLSSSQTTFFREQGYLRLDRVVEPHEVEALKGLLQTLFTNGAGYSE
                     GAQFDLVGSDDSVTQPMLPQIINPSNYARELLRTSFFKTASEIAHQLLGPHARFSDDH
                     VLMKPAKTGAATPWHQDEAFRDPRYETREISIWLALQPVDRRNGCMEFIPGSNLGEVL
                     PHRSPNGNSSVHALECFDGFDAEAAVPCPLPAGGCTIHSGRTLHFAGPNLSDLPRYAY
                     VLIFNLPPLPVRESRDFPWLADRQTQHAERNRRWRSHGGFAVEAVRWVRKLDPRDFQR
                     LLYDVRRARAAVARAFRH"
     gene            5713..6780
                     /locus_tag="VKQ27_03775"
     CDS             5713..6780
                     /locus_tag="VKQ27_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88078.1"
                     /translation="MSVPAPGAIDCDIHPALPSMQVLVPYFDEYWRAHILMRGLERDN
                     YTASAFPPNAPINCRPDWQPPKGAGGTDLDLLRSQALDAFGTRYAICSVLHGAQSIFS
                     EDLSAAMCRAINDWIAAEWLAKDDRLRASIVVPMHSPELAAKEIERVAPDQRFVQVLM
                     WEMAELPLGRRIHWPIYRAADSFDLPIGIHAGSSYRHPPTNLGWPSYYLEDYVSWSTG
                     FAGVLNSLITEGVFVEFPRLKVVLMESGVTWLPGWMWRAEKTWRGVRAEVPWLEKSPI
                     AYAREHVRLTIQPFDAPPKAEQLLRIIDEIGSDEMLLFSTDYPHWHFDEKDALPDGLP
                     DHLLRKILVDNPLKTYSRLSP"
     gene            6797..7909
                     /locus_tag="VKQ27_03780"
     CDS             6797..7909
                     /locus_tag="VKQ27_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88079.1"
                     /translation="MNVTVVDRKPDTSSQTKLGIVDCDVHPYTKSSADLDPFLPERWR
                     KHRQSIGGRSRSPFSNAPNYPRMSPFTGMRMDSYPKDGTHPGSDLEMMQQQLLDLFNV
                     QYGLMMPLLGRGSDERNVEFGAAMATAANEWEAQVWCDKEPRLKAGIQIPLEYTEAAI
                     KEIELRAGDRRFTHVMIPPRGLEPLGRRRYWPILEACAANGFSIALHLGGTSGHPSTG
                     GGWPSFYHEEHPSYPQSKEALVTSLVIEGVFEHIPNLRVALVEGGFAWLPSLSWRLDK
                     HYKRLREEVPHLKRLPSEYIRSNIWVTTQPIEEPERPEDLLATCEWIGWDRIMFSTDY
                     PHWDQDDPRYAFKAPLTEEHKRMVFRENALAFYGLE"
     gene            7914..8327
                     /locus_tag="VKQ27_03785"
     CDS             7914..8327
                     /locus_tag="VKQ27_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HLY88080.1"
                     /translation="MARHVVAPVSEIAPGQSRIVDIAGRSIGVFNVGGEFYALINRCP
                     HEGAPLCIGFVLGRFTSDRPGEYRLERHGEMLRCPWHGWEFDIKTGQSWCHPESVKAR
                     TYNVEVVPGETLAKGPFVAETVPVAVEQNYVVVEL"
     gene            8440..8835
                     /locus_tag="VKQ27_03790"
     CDS             8440..8835
                     /locus_tag="VKQ27_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY88081.1"
                     /translation="MPESELRTKGRAMRRRLLGDAAVERIDKAVYTDPIMAKFAELTQ
                     ECIFGALWTRPGLDPKIRALICVISDTATGREPELKLHLRMARNEGWTEDELVESILH
                     LLGYVGAPLVREALLVAKEVFAEMRAESK"
     gene            8936..9865
                     /locus_tag="VKQ27_03795"
     CDS             8936..9865
                     /locus_tag="VKQ27_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88082.1"
                     /translation="MSSESWRQTIAKAELLLSEGEAAEDISGIADSVSTFNAALKLAP
                     RAEAPLDWAKTQSRLSAALRALGARIDDPVMLQSAMMACDHALEVYRRDSTPEDWART
                     IALLGAAQVVLGERDSDPAWLNAALNSYRLATAVYTREDAPNDWARMQRNCGAVLSLQ
                     GERTGDTTSLRDAVDHYRAALEEYTRQESPIDVAVAQSNLGHALRLLGERDKDRDLLA
                     EAAAACRAALAVYTREATPLEWAMASTNLANALTALDDAASLDEAVTIYREAITALDG
                     PARLQQRAIARYNLKRAETRLATLRQSPNAPHQ"
     gene            complement(9804..10607)
                     /locus_tag="VKQ27_03800"
     CDS             complement(9804..10607)
                     /locus_tag="VKQ27_03800"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY88083.1"
                     /translation="MEFAGKVALITGGGGGIGRAAAIGFARRGAKVVVVDVDATLGEA
                     SAELVKQRGGDARFVRADVTKSADVQAYVKATVDAYGAIDCFFNNAGIEGKAAPTQEY
                     DEAIFDAVIGVNLKGVFLGMRHVLPVMLKQGHGAVVNTASVAGLFGGPGMAPYVASKH
                     GVLGLTKVASADVARYGVRVNAVCPGPVETRMMRSLEAQRNPSDPQLVHDTNAAGTPT
                     GRYATPEEVANVVMYLCSDFSSDITGTHIVIDGGRSGTGGASPAAFRPV"
     gene            complement(10620..11543)
                     /locus_tag="VKQ27_03805"
     CDS             complement(10620..11543)
                     /locus_tag="VKQ27_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLY88084.1"
                     /translation="MLGLVTTPEGKAPMELRELPGPVVQRNEALVEVRAFSLNRGELR
                     SIRNNGEGWVPGQDVAGVVVQQAADESGPPLGARVVALTDEFGWAQRVAVLSHRMAVL
                     PDGVSFAQACTLPVAGLTALRTLRHGAPLLGKRVLITGAAGGVGTLAVQLAARSGARV
                     TAVVGRADRAAGLRELGALEVIEGIDKAEGRFGLILEAAGGASLAQAIKLVEAKGVVV
                     VYGNSSGEPTTISFADFRSAPNSRVQGFSYFYSEAEDRFAPDLALLVSLIADGSLKPQ
                     IGVERSWREFAAVAEQLRDRRVAGKAVFLVD"
     gene            11605..12954
                     /locus_tag="VKQ27_03810"
     CDS             11605..12954
                     /locus_tag="VKQ27_03810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88085.1"
                     /translation="MRQMVMVGFLQAQNCTNLVSSWRHPESRDDSMSADYYKTIGRVL
                     EEGKFHLGFFDDRLAMPDRYGNDHSHTVEHGIRCVKMDPITVLTVMGTATERLGLGAT
                     CSTTYFEPFHVARVFATLDLMTNGRAAWNVVTSMNDGEALNMGHEEHLEHDSRYDRAD
                     EFMEVVLGHWNSWDDDAIALDRETGLFAYPNKVRRLDHQGKWFRSRGPFTVPRSPQGH
                     PVVIQAGQSGRGKHFSARWGEVVFVSTHTSMEHGRQLYREMKEDVASQGRDPDKFFIT
                     PSAYVVCAATKAEAEDKMALIETLATDEDALSLLSEAMNFDFASKGIDEPFTDEELEN
                     VSGTQSMRDRVLRASGIANPTARDFIKFSHRARPNKPFVGGPKEVADGLEQWFTEPVA
                     DGFVLAATHVPGTYIDFVKHVVPELQRRGLYHKDYTGATLRENLGIPVPPPTWSNAR"
     gene            12951..13991
                     /locus_tag="VKQ27_03815"
     CDS             12951..13991
                     /locus_tag="VKQ27_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HLY88086.1"
                     /translation="MKLGLSIGYSRAHLDLPVALVQRAEELGYDSVWTAEAYGTDAVT
                     PLAYLAAVTKRIKLGTGIMQLAARTPANAAMCAATVDALAGGGRFIAGLGVSGPQIVE
                     GWYGQPWGRPYYRYKDYVAIMRKIFQREAPVTHDGREISLPYTGEGAAGVGKPLKSIL
                     HMNPGIPIYLGTGNESTVKLTAEIADGWLPLGFVPGSLPEYLPWLEEGFRRAGNGKSL
                     SDFAIQASVHVEIDNDVKAALARLKPEVALYVGGMGHRDTNFHKDMMIRRGFKDAAER
                     IQELYLAHRKDEAIAAVPDEWVDMKSLVGPPDRIRQRFRAWEDSGATSVTVRSRQAEA
                     IEVMAQAARSNQ"
     gene            complement(14076..14339)
                     /locus_tag="VKQ27_03820"
     CDS             complement(14076..14339)
                     /locus_tag="VKQ27_03820"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HLY88087.1"
                     /translation="MTIVYFRDLPPESVSPAISSRHAEVAPEPGRGGQEPGRNLWLTW
                     LREWRRRGRSRTSLSELNDRMLKDIGVTYAEAEHEANKPFWLP"
     gene            14430..>14615
                     /locus_tag="VKQ27_03825"
     CDS             14430..>14615
                     /locus_tag="VKQ27_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HLY88088.1"
                     /translation="MRRADRLFDIIQILRLARTPITAATIAAELEVTPRTVYRDIATL
                     QSRRVPIDGEAGVGYVLR"
BASE COUNT         2740 a         5372 c         4151 g         2352 t
ORIGIN      
        1 accgaggaca tggttgggac tatggggagc ggccagggca gggtcaaggc gcgcgtagcc
       61 gccgccccga tgacacagaa gaacttgcat cgtgtgcaat tccattcggc tgaatgggac
      121 gccacaattc aattccgtga aatccgtgca gtgggtcgca gagcgatcca atcggcgcaa
      181 tccgggtgat ctctttgatt gctgcatgag accagcggcg cagcgcacga cttcgctgtt
      241 accaccgaag ccggcgcgcc gcgaccgcag ccaaggcgct gcgttcaaat ccgccctgcc
      301 agcgcctcca gttgatcggc gcacagcgac catccttgat ggaagcccat cttctcgtgc
      361 gcctcccggt cctccaccga ccagtgtcgc acccgcgccg tatagcgcgt cccctcgccc
      421 tccggctcga acgtcaggac caacgtcatg aacggcttct gcgaaggctc ccacgccgag
      481 acgaacgcat ccgtgaccac cagccggcgg ttcggcacca cctccagata cacaccgtgg
      541 cacgggatat ctccgccatc cggcccccgc atcaccacca ggttcgaacc gcccggccgc
      601 acgtccagct ccgcctccgg cgtcgtatac ggcaacggcg cgaaccactg cttcagcagc
      661 gcaggatccg tccatgcgcg atacagcttc tccggcggcg cggcgatcag ccgtgtcaga
      721 accaggtcgc gtccttcagc actcgttgtc atgtgtcact ctccttgtgg ataccccaag
      781 gacgcacgac ccacccctca gccgacaccc agccgatcca aatgcatgcg aatatgcgct
      841 gcttcagccg gcgtgttcgc gagcgcgatc gcgcggtcga acgcgacgcg cgcctcctcg
      901 cctcgcccga gctgcaacag cagcccgccc ttcaccccga agaagtggaa atacccagcc
      961 agcctctccg ccagcggctc gatcatcgcc agagccgcct cggccccctg caccttgctc
     1021 acggcaacag cgcggttcag cgtcaccacc ggcgatggct gcagccgctc caacgccgca
     1081 tacagcgcat cgatctgcgc ccagtcggtc tcctccggcc gcgccgcgcg cgcatgcagc
     1141 gccgcgatcg ccgcctgcac ctggtagaca cccggcgccc gatgccgcat cgccttgtcg
     1201 atcagcgcca gcccctcggc gatcagcttg ccgtcccaca gcgagcggtc ctggtcgtcc
     1261 agcagcacga tctcgccggc cgcatcgaac cgggccgccg cccgcgcatg ctgcagcagc
     1321 atcagcgcgg tcagccccat cacctccggc tccgccggaa acagccgcag cagcagccgc
     1381 gccagtcgga tcgcctcgtc gcacagcgaa gtccgcgccg aatagccctc gttgaacacc
     1441 aggtagatca tcgccgcgac agcagccagc cgctccgccc tctcaaccgc ccccggcgtc
     1501 tcgaacggca catccgcccg agcgatccgc gacttggcgc gcgtgatccg ctgctccatc
     1561 gccgcgtcgc tcaccaggaa cgcccgcgcg atctgtgcca ccgtcagccc cgacacgatg
     1621 cgcagcgcca gcgcgatctg ctgcgtcgcc ggcagctccg gatgacagca gatgaacaag
     1681 agccgcagca cgtcatcgcg gtagtgcgac ccgtccagcc gctccgccag cggcgtctct
     1741 acgtcctcca ggtcggacag cgtctcttct tctggcagcg gatcctgccg ggcccgccgc
     1801 cgcgccccat ccagcgccgc gtttcgtccc accaggatca gccacgccgc cggatcacgc
     1861 ggcggcccat tcctgggcca gttttgcagc gcccgcaggc acgcctcctg gtacgcctcc
     1921 tccgcagtat ccagatcgcg gaaataccgc agcagcgccc cgatcgcctg aggccgtgcc
     1981 gcggtcagcg ccccgttgat ccaggtaagg tcagtcatcc cgcacgagga tcggcatgat
     2041 acagcgccac cggccgtatc tcgtacgacc cgcctgggtt cacagccgcc agttcgcgcg
     2101 ccgcgtccag cgcctcatcc aggttggcga catccagaat gtagaacccg agcaactgct
     2161 cctttgtctc tgcaaatggt ccgtcgatca ccagcggcgg atcttgcgtc ttccgcagcg
     2221 tggtcgccga tgtcgtcggc aatagccgca gcgatggccc cagcttcccc tcgcgcgcca
     2281 acttctgctg cacgacgccg aggcgcgcca tcacggctgc gtcctcttcc ttgctccagg
     2341 accagacaac gtcctcgtcg ttgtagcaaa gcacggcata catcatgtgc tggtactccc
     2401 gctgtccccc aaggacgccg gagtgtgcct gctcccgaca ggcgagtgca aaaatccagt
     2461 gtcagatcag cgccgcaccg ctcccggcta atccgcgatc cggcccaccg cgccctctgc
     2521 gccggactcg ccggccgcca ggctctcgtg ccgcatggcc agctgcagca gcgtgttccg
     2581 cacgtcgact gatgcgatgc cagccgccaa ctgccgatac tgcgcggcgc gcatacgccg
     2641 ctctgccggc gagagttccg gaaccagaca aggatgatcg gaatcagtcg aatgaattaa
     2701 catccgtcaa tttccgatca ccgcgacggc agcgtctatc cgggattcta cgtaggatgc
     2761 gcctggtagg atgcgcgggg ccctgacgat cagccgatcg ccttgtagta atacgtcgtc
     2821 gaacaaagcc gcccatcagg ccagagcgcg tagtccggca cctcccccac gcggttccag
     2881 cccattcgct gatacagcct ctccgcgtcg ctgccggtcg cagtatccaa caccaacacc
     2941 gtcttgccgg ccgcaagcgc caccgcctcg cccgcgcgca tcagcagccc gccaacacca
     3001 cgccgccgtg cacggcgatg caccagcatc ttggcgatat ccgcgcgatg aggttggttt
     3061 tccggctgtg cgaacacgac ctggacagtg ccaataatgc cgtgcgcctc gtcctcagcg
     3121 accagcagca cacggtctcc ccgtgccaca ccatcagcca cgccacgcca gaaatcctgt
     3181 gccttcacat cgcacaacgg cgccatgaag ctgaccgacg ccccgccgtg cacgcagtcg
     3241 atcagcacgt cgcaaagcgc ggcattgcag ccataagcct cttccgccgt cagttggcga
     3301 actgaaacac ccatcgcgtt catcggaagt tccgctgttg aggattgata agcgccacca
     3361 ggtgccgagc cgccgtgcgc ccccgattgc ggaacccgat cggctgatca acccgcatcg
     3421 ccaggcagtc gccggcctca agctgatgca cctgctcgcc gacacgcacc tcgatcgcgc
     3481 cctcgatcac ccacacctgc tgatccaccg gcggttcgcg gaacccgctg tcgtaggcaa
     3541 cccgagcccc acccggcagc accacctcca ccaactcgat cggcgaagga aacccagccg
     3601 gcgacaggtt gcgccgcaca taccccgtct ccggatcgga ccacgccgtc tgctcgcctc
     3661 gccgcgcaac aggctgtgca tccacccgcg cctcctcggc aaacatcgag gccagcgtca
     3721 cgcccaacgc cgccgatagc ctgtcgagca cgctcgctgt cgggctgctt tccgcccgtt
     3781 cgatcagcga gatcatcgac cggctcaccc ccgaccgctt cgccagctcg tccagcgtga
     3841 gcccgcgctc cgcgcgaagc gccttcaacc ggtcagcgac acgacgttcg atatcggcag
     3901 aaggctccat agacgattgg aatatctttc caatatactg gatgtcaact ccgtgtcggc
     3961 cgctgcactg accgagccgc cggggacccg aactggcgcc tcctcttccg ccccgactat
     4021 cccctgatcg gccgcctccc gacgcctggc ggaactgcgc ggcgctatga ccgccccggc
     4081 ctcagtcgaa aggctgccgc tgtgagccac cctgcgcacc ggcatcgttc acctgggcat
     4141 cggtgccttc gcccgcgccc atctcgcctc gtatgcacaa acgtagcttc gagatgaccc
     4201 cgcgtaaggc atctgcggcg tcagcctgca cagccccgat acacgcgacg cccttacccg
     4261 caggacaatc tgtatacacg cgctgaacgc ggcggcagcg gcgaacgtct gcaccgccct
     4321 gaccgatctc cttcccggcg ccgacgccac catcaaccgg ttgctcgacc cggcaatccg
     4381 catcgtcacg ctgaccgtaa ccgggaaggg ctaccacggc ccgctgccct cgctcctggc
     4441 ccccgccccg cagcaccgcc gcgcagcgaa catcgccaat cacgtcgtcg tgttcaagca
     4501 ggccgcgatc atcgagcaag gcccgcccag accgatcttt gaagccccac ttgagcgcac
     4561 gcgcgatttc ctgagccatc tgggctggga aggttgaatt tcttccagcc gtactcgacg
     4621 gaatccgatc gacattctca ccttcaatca cgcaggagtg gggtccacca tgcctcgcct
     4681 ttggctaaag tccgaagtgg cagggcaact tcctacgggt cgcaggccca agctgaaggg
     4741 aagcgaacgg tggcatcgtt tcaactgtca agctctcaaa caacgttttt cagggagcaa
     4801 ggttatctgc ggctcgaccg ggtcgtagaa ccgcacgaag tagaggcact gaagggactg
     4861 ctgcagacgc tcttcaccaa tggagcggga tatagcgaag gggcgcaatt cgatcttgtt
     4921 ggttcagacg atagtgtaac gcaaccaatg cttccgcaaa tcatcaatcc cagcaattat
     4981 gcccgggaac tgttgcggac cagcttcttc aagaccgcca gtgagatcgc gcaccagctc
     5041 cttgggccgc acgcccgctt ctcagacgac cacgtcctga tgaagccggc caaaacagga
     5101 gccgcgaccc cgtggcacca ggacgaggct tttcgcgacc caaggtacga aactcgcgag
     5161 atcagcatct ggctcgcgct gcagccggtc gatcgccgga acggatgtat ggaattcatc
     5221 cctgggtcga accttggcga agtcctgccg catcgttcgc ccaacgggaa cagcagcgtg
     5281 cacgccttgg agtgcttcga tggatttgac gcagaggctg cagtgccgtg cccgcttcct
     5341 gccggcggct gcacgatcca ttcagggcgg acattgcact ttgccggccc gaacctctcc
     5401 gacctgccac gttatgccta cgttctgatc ttcaatctgc cgccgctgcc ggtgcgcgaa
     5461 tcccgtgact tcccgtggct tgccgaccgc cagacacagc atgccgagag gaaccgccga
     5521 tggcgctctc acggtggctt cgcggtggaa gccgtgcgct gggtacgcaa gctcgatccg
     5581 cgcgacttcc agcgtttgct gtacgatgtc cgtcgcgcgc gtgccgcggt agcccgggct
     5641 ttccgccact gacccggcat tgaacccacc gccgccccga cctatcctca gggaacaaac
     5701 gaggagcggc ccgtgagcgt tcccgcgccc ggcgcgattg attgcgacat ccacccggcc
     5761 ctgcccagca tgcaggtgct ggtgccatat ttcgacgaat attggcgtgc gcacatcctg
     5821 atgcgcggcc tcgagcgcga caactacacc gcctccgcct tcccgcccaa cgcgccgatc
     5881 aactgccgcc ccgattggca gccccccaag ggcgccggcg gcaccgatct cgacctgctc
     5941 cgctcacagg cgctcgacgc attcggcacg cgctacgcca tctgcagcgt gctgcacggc
     6001 gcgcaatcca tcttcagcga agacctctcc gccgcgatgt gccgcgcgat caacgactgg
     6061 atcgccgccg agtggctggc gaaggacgat cgcctgcgcg catccatcgt cgtgccgatg
     6121 cacagccccg agctcgccgc caaggagatc gagcgcgtcg cccccgacca gcgcttcgtc
     6181 caggtgctga tgtgggaaat ggcagagctg ccgctcggcc ggcgtatcca ctggccgata
     6241 taccgcgctg ccgacagctt cgacctgccc atcggcatcc atgccggcag ctcctatcgc
     6301 cacccgccca ccaacctggg ttggccgtcc tactacctgg aagactacgt ctcctggtcc
     6361 accggcttcg ccggcgtgct gaacagcctg atcaccgagg gcgtgttcgt cgaattcccc
     6421 cgcctcaagg tcgtgctgat ggaatccggc gtcacctggc tacccggctg gatgtggcgc
     6481 gctgaaaaga cctggcgcgg cgtgcgcgcc gaggtgccct ggctggagaa atccccgatc
     6541 gcctacgcgc gcgagcacgt gcgtttgacc atccaaccgt tcgatgcgcc cccaaaagcc
     6601 gagcagttgc tgcgcatcat cgacgagatc ggatcggacg agatgctgct gttctcgacc
     6661 gactacccgc actggcattt cgacgagaag gatgcgctgc ccgatggcct gccggaccat
     6721 ctgctgcgca agatcctcgt cgacaatccg ctgaaaacct attcccgttt atccccgtga
     6781 atctggagag catccgatga acgtcacggt cgttgatcgt aagcctgaca cctcgtcgca
     6841 gaccaagctc ggtatcgtcg attgcgacgt gcatccctac accaagtcgt ccgccgatct
     6901 ggatcccttc ctgccggagc gctggcgcaa gcatcgccag tcgatcggtg gccggtcgcg
     6961 ctcgcccttc tccaacgcgc cgaactatcc acgcatgtcg ccgttcaccg gcatgcgcat
     7021 ggattcttat ccgaaggatg gcacgcaccc tggctccgac cttgagatga tgcagcagca
     7081 gttgctggac ctgttcaacg tccagtacgg cctgatgatg ccgctgctcg gccgcggctc
     7141 cgacgaacgc aacgtcgaat tcggcgcggc gatggccacc gcggcgaacg aatgggaagc
     7201 gcaggtgtgg tgcgacaagg agccgcgcct gaaggccggc atccagatcc cgctggaata
     7261 caccgaagcc gcgatcaagg agatcgagct ccgcgccggc gatcgccgct tcacccacgt
     7321 gatgatcccg ccgcgcggcc tggagccgct gggccgccgc cgctactggc ccatcctgga
     7381 agcctgcgcc gccaacggct tctccatcgc gctgcatctc ggcggcacgt ccggccatcc
     7441 ctccaccggc ggcggctggc cctcgttcta tcacgaggag cacccgtcct atccgcagag
     7501 caaggaagcg ctggtcacct cgctggtgat cgagggggtg ttcgagcaca tccccaacct
     7561 gcgcgtcgcc ctggtggaag gcggcttcgc ctggctgccc tcgctgagct ggcgcctcga
     7621 caagcactac aagcgcctgc gcgaggaagt gccgcacctc aagcgcctgc cctccgagta
     7681 tatacgctcg aacatctggg tgaccaccca gccgatcgag gagcccgagc gtccggaaga
     7741 cctgctggcc acctgcgaat ggatcggctg ggaccgcatc atgttctcca ccgactatcc
     7801 gcactgggac caggacgatc ctcgctacgc cttcaaggcg ccgctgaccg aagagcacaa
     7861 gcggatggtg ttccgcgaga acgcgctggc gttctacggc ctggagtaag cctgtggccc
     7921 ggcacgtcgt cgcacccgtc agcgagatcg cgccgggcca gagcaggatc gtcgatatcg
     7981 ccggccgctc catcggcgtg ttcaacgtcg gtggcgaatt ctatgcgctg atcaatcgct
     8041 gcccgcacga aggcgcccca ctctgcatcg gcttcgtgct cggcaggttc acctcggaca
     8101 ggcccggcga ataccgcctg gaacgccacg gcgaaatgct gcgctgcccc tggcacggct
     8161 gggaattcga catcaagacc ggccagtcct ggtgccaccc ggaaagcgtg aaggcccgca
     8221 cctacaacgt cgaggtggtg ccaggagaaa cgctggcaaa gggcccgttc gtagcagaga
     8281 cagtgccggt ggcagtggaa cagaactacg tggtagtgga actgtagccc cgggccgcaa
     8341 cggcccccca agcgcccgac ccagccaggc aatctcgggc cagtagagcc tacaccgcgg
     8401 gatcgccctg gcgccccgtc catgaaggag caaccaccaa tgcccgaaag tgaactgcgc
     8461 acgaaaggcc gcgcgatgcg gcgccggctg ctaggtgacg ccgcggtcga gcgtatcgac
     8521 aaagccgtct acaccgatcc gatcatggcg aagttcgccg aactgacgca ggagtgcatc
     8581 ttcggcgcgc tatggacccg ccccggcctc gacccgaaaa tccgcgccct gatctgcgtc
     8641 atctccgaca ctgccaccgg ccgtgagccc gagctgaagc tccatctccg catggcccgc
     8701 aacgaaggct ggaccgaaga cgaactggtg gaatcgatcc tccacttgct cggctacgtc
     8761 ggcgctccac tggtccgcga ggcgctactg gtcgccaagg aagtcttcgc agagatgcgc
     8821 gcagaatcaa agtgattcgc agacctcgcc cccgcgtcat ggtcgggctc ggcccgacca
     8881 cccacgactt cgccgccgcc cccacggccg cggccgctca cgtcgagaac cacgcatgag
     8941 ctccgaatcc tggcgccaga cgatcgccaa agccgagctg ctgctgtcgg aaggcgaagc
     9001 cgcggaagac atctccggca tcgccgactc cgtctccacc ttcaacgccg cgctgaagct
     9061 cgcgccgcgc gccgaagccc ctctcgattg ggcgaagacg caaagtcgcc tgtccgccgc
     9121 cctgcgagca ctcggcgcgc gtatcgacga cccggtgatg ctgcagtcgg ccatgatggc
     9181 ctgcgaccac gcgctggaag tgtaccgccg cgattccacc cccgaagact gggcgcgtac
     9241 catcgccctg ctcggcgccg ctcaggtcgt gctgggcgaa cgcgacagtg accctgcctg
     9301 gctgaacgcg gctctgaaca gctaccgcct cgccaccgcc gtctacaccc gtgaagatgc
     9361 acccaacgac tgggcgcgca tgcaacgcaa ctgcggcgcg gtcctgtcac tgcaaggcga
     9421 acgcaccggc gacaccacct cgctgcgtga cgcagtggac cattaccgcg cggcactgga
     9481 ggaatacacc cgccaggaat cgccgatcga cgtggcggta gcgcaaagca acctgggcca
     9541 cgcgctgcgc ttgctgggcg agcgcgacaa ggaccgcgac cttctggccg aggcagccgc
     9601 cgcctgccgc gccgcgctgg ctgtctatac gcgcgaagcc acgccgctcg aatgggcaat
     9661 ggccagcacc aatctggcca acgcgcttac cgcgctcgat gacgcggcct cgctggacga
     9721 agccgtcacc atctaccgtg aagcgatcac cgcgctggac ggccccgccc gcctgcaaca
     9781 gcgcgcgatt gcccgctaca acctcaaacg ggccgaaacg cggctggcga cgctccgcca
     9841 gtccccgaac gccccccatc aataacgatg tgcgtccccg tgatatcgct ggaaaaatcc
     9901 gagcacagat acatcacgac attcgccact tcctccggcg tcgcatagcg acctgtcggc
     9961 gtccccgccg cgttggtatc gtgcaccaac tgcgggtcgc tgggatttcg ctgcgcttcc
    10021 agcgaacgca tcatgcgcgt ctccaccggc ccgggacaaa ccgcgttcac cctcaccccg
    10081 taccgcgcca catcagccga tgcgaccttg gtcagcccca gcacgccatg ctttgaagcg
    10141 acataaggcg ccatccccgg cccaccgaac aacccggcca ccgatgctgt attgacgacc
    10201 gcgccatgcc cctgcttcag catcaccggc agcacatgcc gcatgccaag gaacacgccc
    10261 ttcaaattga cgccgatcac ggcatcgaag atagcttcgt cgtattcctg cgtcggtgcc
    10321 gccttcccct cgatgccggc gttgttgaag aagcagtcga tcgcgccata cgcatcgacc
    10381 gtcgccttca cataagcctg cacgtccgcc gacttcgtca catcagcccg cacgaaccgc
    10441 gcatcgccac cgcgctgctt caccagctcc gcgcttgctt caccgagcgt cgcatccaca
    10501 tcgacaacaa caaccttcgc cccacgtctc gcgaaaccaa ttgcagccgc gcgcccgatg
    10561 ccgccaccgc cgcccgtgat caacgccacc ttgccggcaa attccatcga cgtttccccc
    10621 taatcgacca gaaacactgc tttgccggca acgcgccgat cgcgcaactg ctccgccacg
    10681 gcggcaaact cgcgccagct tctctccacg ccaatctgcg gcttcagcga cccatcagcg
    10741 atcagcgaca ccagcagcgc gaggtccggc gcgaaccgat cttccgcctc cgaatagaaa
    10801 taactgaagc cctgcacgcg agaattcggc gcactgcgaa aatcggcaaa gcttatcgtc
    10861 gtcggctcgc ccgacgaatt gccatagacc acgacaacgc ccttcgcctc caccagcttg
    10921 atcgcctgcg ctaaggacgc gccgcccgca gcctccagga tcagcccaaa ccgcccttcc
    10981 gccttgtcga tcccctcgat cacctcgagc gcgccaagtt ccctcaagcc cgccgcccga
    11041 tccgcacgac ccaccaccgc agtgacccgc gcccccgatc gcgccgcaag ctgcaccgcg
    11101 agcgtgccaa ccccgcccgc cgccccggtg atcagcacgc gcttacccaa cagcggtgcg
    11161 ccatggcgca acgtgcgcaa tgctgtcagc cccgccaccg gcaacgtgca cgcctgcgca
    11221 aagctcaccc catccggcaa caccgccatg cgatgcgaca acaccgccac ccgttgcgcc
    11281 cagccaaact catcagtcag tgccacaaca cgcgcgccca acggcggccc gctctcatca
    11341 gccgcctgct gcaccacaac gcccgcgaca tcctgccccg gcacccaccc ctcgccgttg
    11401 ttgcgtatcg accgcagctc accccggttc agcgagaacg cgcgcacctc caccagcgcc
    11461 tcattccgct gcaccaccgg tccgggcagt tcgcgcaatt ccatcggcgc cttgccctct
    11521 ggcgtggtga cgagaccaag catcggattg ccctccactg ctgttccgcg cgatgatact
    11581 cgcaagtagg gacggagcgt taagatgcgc cagatggtca tggttggatt cctgcaagcg
    11641 cagaattgca ccaacctcgt cagctcgtgg cgccatccgg aatcgcgcga tgactcaatg
    11701 tccgccgact actacaaaac tatcggtcgc gtgctggagg aaggcaagtt ccacctgggt
    11761 ttcttcgacg atcgtctggc gatgcccgac cgctacggca acgaccatag ccacaccgtg
    11821 gagcacggca tccgctgcgt gaagatggac ccgatcacgg tgctcacggt gatgggcacc
    11881 gcgaccgaac gattgggtct cggcgcgacc tgctcgacca cctatttcga gcccttccac
    11941 gtcgcacgcg tgttcgcgac gctggatctt atgaccaacg gtcgcgccgc ctggaacgtc
    12001 gtcacctcga tgaacgacgg cgaagcgctg aacatgggcc acgaggaaca cctggaacac
    12061 gattcgcgtt acgatcgcgc cgacgaattc atggaagtcg tgctcggcca ctggaattcc
    12121 tgggacgacg acgcgatcgc gctcgatcgc gaaaccggcc tgttcgcata ccccaacaag
    12181 gtccgccgcc tcgaccacca ggggaaatgg ttccgttccc gcggtccctt cactgtgcca
    12241 cgttcgccgc aaggccaccc ggtcgtgatc caggccggac aaagcggccg aggcaagcat
    12301 ttctcggccc gctggggcga agtggtgttc gtctccaccc acaccagcat ggaacacggc
    12361 agacagctct atcgcgagat gaaggaagac gtcgccagcc aaggccgcga ccccgacaaa
    12421 ttcttcatca cgccatccgc ctacgtcgtc tgcgccgcga cgaaagcaga agccgaggac
    12481 aagatggcgc tgatcgaaac gctcgcgacg gacgaagacg cactttccct gctgtccgaa
    12541 gccatgaact tcgacttcgc ctccaagggt atcgacgaac cgttcaccga tgaggaactg
    12601 gagaacgtct ccggcacgca gtcgatgcgt gatcgcgtgc tgcgcgccag cggcatcgcc
    12661 aacccgacag cgcgcgactt catcaagttc agccatcgcg cgcgtcccaa caagccattc
    12721 gtcggcggcc ccaaggaagt ggcagatggc ctggaacaat ggttcaccga gccggtcgcc
    12781 gatggcttcg tgctcgccgc aacgcacgtg cccggcacct acattgattt cgtcaagcac
    12841 gtggtgcccg aactgcaacg ccgcggcctg taccacaagg actacaccgg cgcgaccttg
    12901 cgcgaaaatc tcggcatccc cgtgccaccg ccaacctgga gcaatgcgcg atgaagctcg
    12961 gcctttccat cggctattcc cgcgcgcatc tcgatcttcc cgtcgccctc gtgcagcgcg
    13021 cggaagagct cggctatgac agtgtatgga cagccgaagc ctacggcacc gatgccgtga
    13081 cgccgctcgc ctacctcgcc gccgtcacca aacgcatcaa gctcggcacc ggcatcatgc
    13141 agcttgccgc gcgcacgccg gccaatgccg cgatgtgtgc ggccacggtg gatgcgcttg
    13201 ccggtggcgg ccgcttcatc gcgggcctcg gcgtgtccgg cccgcagatc gtcgaaggct
    13261 ggtacggcca accctggggg cgcccgtatt atcgctacaa ggactacgtc gcgatcatgc
    13321 gcaagatctt ccagcgcgaa gccccggtca cccatgacgg ccgcgagatt tcgctgccct
    13381 acaccggcga aggtgccgcc ggcgtcggca agccgctcaa gtccatcctg catatgaacc
    13441 ccgggattcc gatctatctc ggcaccggca acgaatccac cgtgaagctc acagcggaaa
    13501 tcgccgatgg ctggctgccc ctcggtttcg ttcccggctc gctccctgaa tatctcccct
    13561 ggctggaaga aggtttccgc cgcgccggca acggcaagag cctgagcgac ttcgccatcc
    13621 aggcttcagt ccacgtcgaa atcgacaacg acgtgaaggc cgcgcttgcc aggctgaaac
    13681 cggaggtcgc gctctacgtc ggcggcatgg gccaccgcga cacgaacttc cacaaggaca
    13741 tgatgatccg ccgcggcttc aaggacgctg ccgagcgcat tcaggaactc tatctcgcgc
    13801 accgcaagga cgaagcgatc gccgcggtgc ccgacgagtg ggtggacatg aaatcgctcg
    13861 tcggcccgcc ggacaggatc aggcagcgct ttcgcgcctg ggaagattcc ggcgccacca
    13921 gcgtcacggt gcgttcacgg caagccgaag ccatcgaggt gatggcgcaa gccgctcggt
    13981 cgaaccagtg atccggtcgc ggacgcgcgc ccatatcggg ccaccggcgt gagataatgc
    14041 cggcggtccc gatgggcacc ccgaaacaac gttcgtcagg gtagccagaa cggcttgttg
    14101 gcctcatgct cagcttcagc ataggtgacg ccgatgtcct tcagcatgcg gtcgttgagc
    14161 tccgagagtg atgtccgaga gcgaccgcgc cggcgccact ctcgcagcca ggtcagccat
    14221 agattgcgcc ccggctcttg cccgccgcgg cccggttcgg gtgcgacttc ggcatgccgc
    14281 gatgatatgg ccggggagac gctctcgggc ggcaggtcac ggaaataaac gatggtcatg
    14341 acaacactcc ttcatctacc gatgcctggg aacgacatag gaaccgcctg ctgacagcgt
    14401 tctgtcagca atgacacggg gggcatcaaa tgcgccgtgc ggaccggctg ttcgacatca
    14461 tccagatcct gcgtctggcg cgcactccga ttactgcggc gactatcgcg gccgagctgg
    14521 aggtgacgcc gcgcaccgtc tatcgcgaca tcgccacatt gcagtcgcgc cgggtgccga
    14581 tcgacggaga agcgggggtt ggctacgtgc tgcgg
//
LOCUS       DATGEM010000120         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_443090, whole genome shotgun sequence.
ACCESSION   DATGEM010000120 DATGEM010000000
VERSION     DATGEM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443090"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VKQ27_03830"
     CDS             complement(<1..287)
                     /locus_tag="VKQ27_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88089.1"
                     /translation="MREVLGMALDGGGVSADVRALLLGVRIDTRNLRNFVEPERLTLK
                     GMGTAFVFRYGVLVLFAASPETERDLITALEGHIIDHVASPEIETASIEIRP"
     gene            complement(444..1034)
                     /locus_tag="VKQ27_03835"
     CDS             complement(444..1034)
                     /locus_tag="VKQ27_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88090.1"
                     /translation="MIPAACYPVYSALAARGAVPASGYLVNVEADCFRREPSLDPIRI
                     QSFHMQELVHVGRPGDVLAFRDRWLESGLALFSAWGLAGTIEPADDPFLGRAAVVMAR
                     SQRQQALKFELQLPITDPSKPTACMSANYHLDSFGRLAGLVFADGTPTHSVCAGFGFE
                     RIALALFRHHGTTLEHWPGEVRDALSLAAPETASRF"
     gene            1104..1520
                     /locus_tag="VKQ27_03840"
     CDS             1104..1520
                     /locus_tag="VKQ27_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88091.1"
                     /translation="MILVVPAEAMHGSEQMRKIPKIADPLQIGARHYRGEAHPFRAGA
                     EVLTSGVSYRVGDRRKILTLPVQTGNRCWDEQAAGDQLGCKGVGGGGAHVALARGIRA
                     SMIARRAGIASVDSISALVMRKPNESSTAKMISTVA"
     gene            1612..2046
                     /locus_tag="VKQ27_03845"
     CDS             1612..2046
                     /locus_tag="VKQ27_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88092.1"
                     /translation="MHPEPSIVRHDEILSSMMAALCVETNQPRTRCDSVTKENEMRIK
                     LKMVALAALVAGPTIMPASALAQSSSNYENHRVYHGRGGHYHRRCGGGNGAVGTVAGG
                     VGGALIGHAVGGGTLGTVAGGVGGALLGRHLDKEHTRSRNGC"
     gene            complement(2155..2898)
                     /locus_tag="VKQ27_03850"
     CDS             complement(2155..2898)
                     /locus_tag="VKQ27_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HLY88093.1"
                     /translation="MQASIQEKLLSALGAAIVVLLFGYLLLVGMTVGIPVRDEQILTL
                     LDYRAPAPRPRRPPPHVRRLKPARAPGKPSPPNLRNKAAAIVTTPTPVPPPIPSPLVS
                     ASKAGTGMAAQSGASDRPGPGEGAGGEGNGTGGGGYGNGDGGGDVPPRLIKGRLKFSD
                     LPADLRDSQIGGTVSVRYSVEVNGGVGDCIVMVSSGNAELDQTTCRLIQQRFRFDPSH
                     DRDGRPVRSMIDENHSWEFDMSQDPRVRR"
     gene            complement(3038..4018)
                     /locus_tag="VKQ27_03855"
     CDS             complement(3038..4018)
                     /locus_tag="VKQ27_03855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HLY88094.1"
                     /translation="MVLGGMLLLCVGLGLALTARMALRIQKAGRDLADSEARYRLLAE
                     HASDLVMRLMPDGTCTYVSPSAAELTGFPVSTYVGYQPMVMLHPDDHATALAALDAVR
                     SGEASTKVTTFRSKHRGGHWLWFEANMRRIGDAGTTEASEVVCILRDISSRKALEEKL
                     TRQATVDELTGLFNRRHFLECFSAEVERARRYGHALTLALIDLDHFKRVNDGHGHAAG
                     DLVLSLTASIIRSTMRTTDIAGRIGGEELAVIMPDTDLDAAHVAAERLRRAISACSFL
                     IAGGQAVHVTISIGLSQAQADEPISSIMSRADAALYAAKTAGRDRVLRAA"
     gene            4037..>4259
                     /locus_tag="VKQ27_03860"
     CDS             4037..>4259
                     /locus_tag="VKQ27_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88095.1"
                     /translation="MLGLLRRYRALSPDVEEPAFFGDDRGQYGVDGFGVAMRLRGECP
                     EAGDPALLVGIVDSEIGKTRDQKASLVGQG"
BASE COUNT          761 a         1343 c         1411 g          744 t
ORIGIN      
        1 ggacgaatct cgatcgatgc cgtctcgatc tcgggcgaag cgacgtgatc gatgatatgg
       61 ccttcgagcg ctgtgatcag atcccgctcg gtctcgggtg aggccgcgaa cagcacgagc
      121 actccgtagc gaaaaacgaa ggcggtcccc atgcccttga gcgtcagcct ctcgggctcg
      181 acgaaattcc ggaggttgcg ggtatcaatg cggacgccga gcagcaaagc ccgaacatcg
      241 gcactgaccc cgccgccatc gagcgccatg cccagaacct cgcgcacgcc cacgctggcg
      301 acgcgcccgg tgccggccgg aagattggct ggccacgttg cgcccgtcat tccggtctcc
      361 gtgtcgctgg tccgcgcctc cgcctctacc ccgccccttg gtggggaggg aagccttgtt
      421 tcggcggcca acgccggcgc gggctagaat cgcgaggcgg tctctggagc ggcgagcgac
      481 aatgcatcgc ggacttcgcc tggccagtgc tccagcgtgg tgccatggtg gcggaagagc
      541 gccagggcga tgcgctcgaa cccgaagccg gcacagaccg aatgcgtcgg cgttccgtcc
      601 gcgaacacga gaccggccaa tcgtccgaaa ctgtcgagat gatagttggc actcatgcag
      661 gccgtcggct tggagggatc ggtaatcgga agctgaagct cgaacttgag tgcctgctgc
      721 cgctgggatc gggccatcac cacggccgcc cgccccaaaa agggatcgtc ggcgggctcg
      781 atcgttccgg caagccccca agccgaaaac aaggcgaggc cagattcgag ccagcggtcc
      841 cggaacgcga ggacgtcgcc gggcctgcct acatgtacaa gttcctgcat gtggaagctc
      901 tggatccgga tcgggtcgag cgacggctca cgccggaaac aatctgcttc gacatttacg
      961 agatagccgc ttgccggaac ggcgccgcgt gctgcgagag ctgagtaaac cggatagcag
     1021 gcggccggga tcaacacgag atccgatgcc agctgcgcgt cggtccaatc ttcgcccgca
     1081 tcatgtttac gcagctggct gcgatgatcc tcgtcgttcc cgcagaagca atgcacggtt
     1141 ccgagcagat gcggaaaatt ccgaaaatag ccgatccgct ccagatcggc gcgaggcatt
     1201 accgaggcga agcgcatccg ttccgcgccg gagccgaggt gctgacgtca ggtgttagct
     1261 atcgcgtcgg tgaccgccgc aaaatcctga ccctgcccgt acagaccggg aaccgatgtt
     1321 gggacgagca ggctgctggc gaccaactgg gatgcaaagg ggtgggaggg ggaggcgctc
     1381 atgttgcgct cgcgagaggc attcgtgcct cgatgatagc aagaagggcc ggaatcgcgt
     1441 cggtcgactc catctccgcg ctggtcatgc ggaagccaaa cgagtcctcg acagcgaaga
     1501 tgatctccac cgtcgcatag gattcccccc ctgtatctcg ttcgggtccg cgatcctgat
     1561 gtaggatttg cgggcgggcc atgatgtatc cgcccgatca agcatgggcc ggtgcatccc
     1621 gagccatcga tcgttcgcca tgacgagata ttatcgagca tgatggcggc gttgtgtgta
     1681 gaaaccaatc aacctcgcac gcgttgcgat agcgtcacaa aggagaatga gatgaggatc
     1741 aagctcaaga tggtcgcttt ggccgccctt gtggccgggc cgacgatcat gccggcctct
     1801 gcgctcgcgc agagctcgag caattacgaa aaccatcgtg tctaccacgg aaggggcgga
     1861 cattatcacc gtcgctgtgg tggcggcaac ggcgcggtcg gcacggttgc tggcggtgtc
     1921 ggcggcgcct tgattggaca tgccgtcggc ggggggacgc tcgggacggt ggccggaggt
     1981 gtcggcggcg cgctgctcgg acggcacctt gataaggagc atacaaggtc gcgtaacggc
     2041 tgttgacgag ccccgccttc ctccgatctg acaaggggga gggcggtgca cctgttctgc
     2101 tgtttggagc cggcagggcc ttggaggtca gtagcggcat ggacttccgc gcggtcagcg
     2161 gcgaacgcgc ggatcctggc tcatgtcgaa ttcccagcta tggttttcgt cgatcatcga
     2221 tcgaaccggc cggccatcgc gatcatgaga gggatcgaag cggaaacgct gctggatgag
     2281 ccggcatgtc gtctggtcca gctccgcgtt tccgctcgag accatgacga tgcagtcgcc
     2341 aaccccgccg ttgacctcca cgctataccg cacagagaca gtgcccccga tttggctgtc
     2401 gcgcaggtcc gctggcaggt cggagaattt gaggcgcccc ttgatcaacc ttggcggcac
     2461 atcacccccg ccgtcgccat tgccatagcc tccaccaccc gtgccattcc cctcgccacc
     2521 cgccccttca cccgggccgg gccgatctga agcacccgat tgtgctgcca ttcccgttcc
     2581 cgctttcgag gcggagacca gcggggatgg tatcggcggc ggcaccggcg ttggggtcgt
     2641 gacgattgcc gccgctttgt ttctcagatt gggtggggag ggtttgccag gagcgcgcgc
     2701 gggcttcagc cggcggacat gcggcggagg ccggcgtggc cttggggcgg gtgcgcgata
     2761 atccaggagc gtcaagatct gctcatcccg aacagggatc ccgaccgtca tcccgaccag
     2821 cagcagatag ccgaagagca ggacgacgat tgctgcgcca agcgcgctga gcagtttctc
     2881 ttgaatgctt gcttgcatgg caccggtcat ttcctcggcg ggcattccgc ctcggtttcc
     2941 agaccaacgc tgcaatgccc gtccagggcc cgggagacag cgaatggccc cgtcctcagg
     3001 tggccgatcc atgcctgcat cggcgcgcgc ctgccggtca ggccgcccgc agaaccctgt
     3061 cacgaccagc ggttttggcc gcatagagag ccgcgtccgc cctcgacatg atcgatgaga
     3121 tcggctcgtc cgcctgggcc tgcgacaggc cgatgctgat cgtcacatgt accgcctgtc
     3181 cacctgcgat caggaagctg caggccgaga tcgcacgccg caggcgctcg gcggcgacgt
     3241 gcgcggcgtc gagatcggtg tcgggcatga tcaccgccag ttcttctccg ccgatgcggc
     3301 cggcgatgtc ggtggtccgc atggtgctgc ggatgatcga ggcggtcagg ctcagcacga
     3361 gatcgcccgc cgcatgacca tggccgtcat tgacccgctt gaaatgatcg agatcgatca
     3421 gcgccagcgt cagcgcgtgc ccgtagcgcc tggcccgctc tacctcggcc gagaagcatt
     3481 cgaggaaatg gcggcggttg aacagtccgg tcagttcgtc gacagttgcc tggcgggtca
     3541 gcttctcctc gagcgccttg cgcgacgaaa tatcgcgcag gatgcagacg acctcgctcg
     3601 cctcggtggt tccggcgtcg ccgatccgtc gcatgttcgc ctcgaaccac agccagtgcc
     3661 cgccccgatg cttggaccgg aaggtggtga ccttcgtcga tgcctcgccg gaacgcaccg
     3721 catcgagcgc cgccagcgcc gtcgcgtgat cgtcggggtg cagcatcacc atcggctggt
     3781 aaccgacata ggtcgacacc gggaaaccgg tcagctcggc cgcggacggc gaaacatagg
     3841 tgcatgttcc gtcgggcatg aggcgcatca ccagatcgga ggcatgctcg gccagcaggc
     3901 gatagcgtgc ctcgctgtcg gcaagatcac gaccagcctt ctggatccgc aacgccatgc
     3961 gcgccgtcaa ggcgagcccc aggccgacgc aaagcaacag catgccgccc aggaccatga
     4021 ttgtgatcgt gttgcggtgc tgggcctgtt gcgccgctat cgcgcgctgt cgccggatgt
     4081 cgaggagccg gcgttcttcg gcgacgatcg cggccagtat ggcgttgacg gcttcggggt
     4141 tgcgatgcgc ctgcgcggag aatgtccgga ggcgggcgat ccggcgctgc tggtcgggat
     4201 tgtcgacagt gagatcggca agacgcgcga tcagaaggcg agcctcgtcg gtcaggggc
//
LOCUS       DATGEM010000121         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1459891, whole genome shotgun sequence.
ACCESSION   DATGEM010000121 DATGEM010000000
VERSION     DATGEM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459891"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VKQ27_03865"
     CDS             complement(<1..1968)
                     /locus_tag="VKQ27_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HLY88096.1"
                     /translation="MIAGLIRWSIANRFLVLLATLMLTAWGIWSVAGTPLDAIPDLSD
                     VQVIIRTTYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGYSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQARLPPQARTSLGPDATGVGWIYEYALVDRSGRMDLSQLRALQ
                     DWFLKYELKTVPNVAEVASIGGMVRQYQIVLQPDKLRAYNITHGKVIDAVRRANQETG
                     GSVLELGEAEYMVRASGYLQSLEDFRKIPLTTSDAGVPVRLGDIAHIQVGPEMRRGIA
                     ELNGEGEVAGGVIVMRSGKNALATIDAVKTKLQKLKPGLPPGVEILPTYDRSNLIKRA
                     VHNLQQKLIEEFVVVALVCLLFLFHIRSAFVAIVSLPLGVLAAFIVMRYQGVNANIMS
                     LGGIAIAVGAMVDAAVVMIENAHKHIEAWHHAHPGEPLRGESHWRVIGDAAAEVGPAL
                     FFSLLIIVLSFIPVFTLEAQEGRLFSPLAFTKTYTMAAAAGLAVTLIPVLMGYLIRGR
                     IPAERSNPLNRLLIVLYRPLLNDVLRFPRSTLLLAVVIVAVTIWPLSRLGGEFMPPLD
                     EGDLLYMPSALPGIGVGKVAELLQQTDRLIKTVPEVASVFGKAGRAETATDPAPLEMF
                     ETTIQFKPRSQWRPGMTPDKLVEELDRVVHVP"
BASE COUNT          386 a          641 c          653 g          379 t
ORIGIN      
        1 tggcacgtgc acaacccggt cgagttcctc gaccagcttg tccggggtca taccgggccg
       61 ccattgactg cgcggcttga actggatcgt ggtttcgaac atttccagcg gcgcgggatc
      121 ggtcgcggtt tccgcacggc ccgccttgcc gaacacgctg gcgacttccg gaacagtctt
      181 gatcaggcgg tcggtctgct gcagcagttc cgcgaccttg ccaacgccga tgccggggag
      241 ggcggatggc atatacagca gatcgccttc gtcgagcggc ggcatgaatt cgccgcccag
      301 ccggctcagg ggccagatcg taacggcgac tatgacgacg gcgagcagca gagtgctcct
      361 gggaaagcgc agcacgtcat ttaaaagcgg ccggtagagc acgatcagca gccggttcag
      421 cggattgctg cgctcggccg gaatgcgccc gcggatcaga tagcccatga gcaccgggat
      481 cagcgttacg gcaaggcccg ctgcggccgc catagtgtag gtcttggtga acgccagcgg
      541 cgaaaacagt cggccctcct gcgcttccaa ggtgaacacc ggaatgaacg acagcacgat
      601 gatcagcagc gaaaagaaca gcgccggccc gacttcggcg gccgcatcgc cgatcacccg
      661 ccagtgggat tcgccacgca gcggttcacc gggatgcgcg tgatgccatg cttcgatgtg
      721 cttgtgcgcg ttttctatca tgaccacggc cgcatcgacc atggcgccga ccgcgatggc
      781 gatacctccc agggacatga tgttggcgtt gacgccctga tagcgcatga cgatgaacgc
      841 ggcgagtacc cccagtggca gcgagacgat ggcgacgaac gccgagcgga tgtggaacag
      901 gaacagcaag cataccagcg ccacgacgac gaattcctcg atcagctttt gctgcaagtt
      961 gtgcaccgcg cgcttgatca gattcgagcg atcgtacgtt ggcaagatct cgacgccggg
     1021 cggcaggccg ggtttgagct tctgcagctt ggtcttgacg gcatcgatgg tggccagtgc
     1081 gttcttgccc gagcgcatga cgatcacgcc acccgccact tcaccttcgc cgttgagctc
     1141 cgcgatgccg cgccgcattt ccggtcccac ctggatatgg gctatatcgc cgaggcgcac
     1201 tggtacaccg gcatcgctcg tcgtcagggg aatcttgcgg aagtcctcca gcgattgcag
     1261 atagcccgac gcgcgcacca tgtattccgc ttcgcccagt tccagcaccg atccgccagt
     1321 ttcctggttg gcgcggcgca cggcatcgat gaccttgccg tgcgtgatgt tgtaggcacg
     1381 cagcttgtcc ggctgcagca cgatctgata ctggcgcacc atgccgccga tgctggcgac
     1441 ctctgcgacg ttcggcaccg tcttgagttc gtatttgaga aaccagtcct ggagtgcgcg
     1501 cagttgcgac aaatccatgc ggccgctgcg atccaccagc gcgtactcgt aaatccagcc
     1561 cacgccggtc gcgtccggcc cgagcgaggt tctggcctgc ggtggcaggc gcgcttgcac
     1621 ctgattcagg tactcgagca cgcgggagcg cgcccaatag agatcggtgc cgtcttcgaa
     1681 caggacgtag acgaaggaat cgccgaagaa cgagtagcca cgcaccgttt tcgcgcccgg
     1741 caccgagagc atcgttgtgg tcagcggata agtgacctgg ttttcgacga tctgcggcgc
     1801 ctggcccgga taggtggtgc ggatgatgac ctgcacatcg gacaagtcgg ggatcgcgtc
     1861 cagcggggtt ccggccaccg accagatgcc ccatgccgtg agcatcagcg tggcgagcag
     1921 caccaggaag cggttggcaa tcgaccagcg gatcaggccg gcgatcattt cttcttcgcc
     1981 ttcatgtcat ccatgccgct gccagggcct tcatgcgcgg gctgcgagga cgaagtcagt
     2041 ccgattctgg tgatttcga
//
LOCUS       DATGEM010000122         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1103504, whole genome shotgun sequence.
ACCESSION   DATGEM010000122 DATGEM010000000
VERSION     DATGEM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103504"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VKQ27_03870"
     CDS             complement(<1..438)
                     /locus_tag="VKQ27_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88097.1"
                     /translation="MFRWFLRRQIARFERTWKYDAAYLRELIEIDPRAVQALGKLQSI
                     SNYRRDVPPAVWAAAGIVSVMTEDCGPCTQLGIDMAERGGVDPVVLRAVVARDFAAMP
                     DEVALTVRFTEATLRHAPEADELREAVVRRFGKRGLVSLAFAML"
     gene            590..1084
                     /locus_tag="VKQ27_03875"
     CDS             590..1084
                     /locus_tag="VKQ27_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88098.1"
                     /translation="MHKTIAGLCACILAGIGAANAAQPCARPIDRTAFDIAGLKSKLM
                     VTAITCSQQDLYNQFVQRFRTDLVTHERNLHGYFARVFGGRAQREHDDYITSLANTLS
                     ESGIQQGTLFCQQNTGIFTEVLALTKGSDLPAYAASKAFPQPVSLVACPEPTRTAQAG
                     SSRR"
     gene            complement(1085..1711)
                     /locus_tag="VKQ27_03880"
     CDS             complement(1085..1711)
                     /locus_tag="VKQ27_03880"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HLY88099.1"
                     /translation="MAGAALTCARGAKAGLPVPPDRRIAFRLMRHGSPIGTHTLEFRG
                     DGNTLLVDIAVEVRVWLGPIPLAHYFHRSRETWSGDRLVSVQGQTDRNGRQLNMAASW
                     DGRGLAVHGTGTSPYVAPSDAYASTYWRKASLFGPLIGTQDGTLNRPSIVEPGVEPVR
                     LASGVQTRAQRYVLSGDMNVELWYDETDAWVGMRFEADDGSTISYQRV"
     gene            1843..>2225
                     /locus_tag="VKQ27_03885"
     CDS             1843..>2225
                     /locus_tag="VKQ27_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLY88100.1"
                     /translation="MLLRHAKSSWDDPKLSDRDRPLNKRGRRAAATMRQAMQDLGLSP
                     DLILVSTARRTQETLAALEPWDETPLIEPLESLYLASAPQLLDVLRGTNETVRSVMLI
                     GHNPGMHELAVSLAGSRESEQIHRL"
BASE COUNT          433 a          811 c          639 g          342 t
ORIGIN      
        1 gagcatggcg aaagcgagtg acaccaggcc gcgtttgccg aaccggcgca ccaccgcctc
       61 ccgcagttcg tcggcttccg gagcgtggcg cagcgtcgcc tcggtgaaac gcacggtcag
      121 cgccacctcg tccggcatcg ccgcgaagtc gcgcgccacg acggcacgca gcaccaccgg
      181 atcgacgcca ccgcgttccg ccatgtcaat cccgagctgc gtgcacgggc cgcaatcctc
      241 cgtcatcacc gacacgatgc ccgctgcggc ccagacggcg ggcggcacgt ccctgcggta
      301 attgctgatc gactgcagct tccccagggc ctgcaccgcc cggggatcga tctcgatcag
      361 ctcacgcagg taagcggcgt catacttcca ggtgcgctcg aaccgggcga tctgtcgccg
      421 cagaaaccat cggaacatgg tctctctcct ttccgtgctc tcaatgcaca gacgaggcag
      481 gacggccccg tgtgacatgg ggcgcagaac ttgaaaatgc gccatcacca gcacttgcct
      541 cgccacgatc ataccggcag agtatcggcc aaccgggggg caattcagca tgcataagac
      601 tatagccggc ctctgcgcct gcatcctggc cggcattggc gccgccaacg ccgcccaacc
      661 atgtgcgcga cccatcgacc gtacggcgtt cgacatagca ggcctgaaaa gcaaactcat
      721 ggtgaccgcc atcacgtgca gccagcagga cctgtacaat cagttcgtcc agcggttccg
      781 caccgacctc gtgacgcatg agcgcaacct gcacggctac ttcgcccgcg tgttcggcgg
      841 ccgcgcgcag cgcgagcatg acgactacat caccagcctg gccaacacgc tgtcggaaag
      901 cggtatccag caaggcacgc tcttctgcca gcagaacacc gggatcttca ccgaggtcct
      961 ggcactcacc aaggggagcg acctgcccgc ttacgcggca agcaaggcct ttccccagcc
     1021 agtaagtctc gtcgcctgcc ccgagcccac gcgcaccgcg caggccggca gcagccgcag
     1081 atagtcagac gcgctgatag ctgatcgtcg agccgtcgtc ggcctcgaag cgcatgccga
     1141 cccaggcgtc cgtttcgtcg taccaaagct cgacgttcat atcgccgctc agcacatagc
     1201 gctgcgccct ggtctgcacg cctgacgcca gccgaaccgg ttcgacaccc ggctcaacga
     1261 tcgacggacg gttcagcgtg ccatcctgcg tcccgatcag tggcccgaac agcgacgcct
     1321 tgcgccaata cgtagaagca tacgcatcgg agggcgcaac gtaaggcgac gtcccggtgc
     1381 catggacggc cagtccgcga ccatcccaac tcgctgccat gttgagttgg cgcccgttgc
     1441 ggtctgtctg tccctgcacg ctgaccagcc gatcgcccga ccacgtctcg cggcttcgat
     1501 gaaaatagtg cgccagcggg atcggcccga gccagacgcg cacctcaacg gctatatcga
     1561 ccagcagcgt gttgccatcg ccgcgaaatt ccaaggtgtg cgtgcctatg ggcgagccat
     1621 gccgcatgag gcgaaacgca atccgcctgt caggcggcac cggaagcccg gccttcgcgc
     1681 cacgcgcaca agtcagcgcg gcgccggcca cgatgaatga tcgccggcag atcatagctg
     1741 tccaggttcc ccaatcacca tgtcagcgcc ctcacactga acccgcttcg gggaggggac
     1801 aagtcccccg ctccgtggca acatcgggaa atgcatcaac ttttgctgct gcggcacgcc
     1861 aagtcgtcct gggacgaccc gaagctgtcc gaccgcgatc gcccgctgaa caagcgaggc
     1921 cggcgggccg ccgccaccat gcgccaggca atgcaggatc tcggcctctc ccccgacctc
     1981 atccttgtct cgaccgcgcg ccgcacacag gaaacgctcg ccgcgctgga gccatgggat
     2041 gaaacaccac tgatcgagcc gctggagtcg ctctacctcg ccagcgcgcc ccagctgctc
     2101 gacgttctcc gaggcaccaa cgagaccgtg cgcagcgtca tgctcatcgg ccacaatccc
     2161 ggcatgcacg aactcgccgt atcgctggcc ggttcacgcg aatccgaaca gatccatcgt
     2221 ctgag
//
LOCUS       DATGEM010000123         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1565476, whole genome shotgun sequence.
ACCESSION   DATGEM010000123 DATGEM010000000
VERSION     DATGEM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565476"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VKQ27_03890"
     CDS             complement(<1..294)
                     /locus_tag="VKQ27_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88101.1"
                     /translation="MSAFDHTGRAAANRHSDRRFHIIMGCIFSGAFILPIAIAWTVIA
                     TEPGRRAAYMAKCQQTGFSEGQCRFLYGERRDADTAALAAIAVGAGVAAGQVAS"
     gene            857..>2326
                     /locus_tag="VKQ27_03895"
     CDS             857..>2326
                     /locus_tag="VKQ27_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY88102.1"
                     /translation="MAGPTEPNPTIRPPDRRKLIAVLYADMVGYSRLIGLDDAGTLQR
                     LRAFRTNLIDPAIEEHGGRIVQTGGDSLLIVFDSIDGAMRCALKVQQRVPVLDSDQPT
                     DQSIRFRIGIDIGDAIADGTDLHGDAVNVVARLQAECPPGGVCVSRSVRDHVHGRLGL
                     AFEALGPLHLKNIARPVEAFVVRLDAPKMPRGVGDAGSQVDRPPAEPAPAYRNNLPQL
                     AHALIGRQRDVAEIEALLSRCRLVTLVGAAGVGKTSLSLQVGANLLSGFPDGVWFVEL
                     APLDQAELIGEAVAAVFGLPVQGERPTTDAIAAFLRSRRVLLILDNCEHVIAAATNLA
                     NALLKTCPGVFLLASSREPLSVGGEHAYPVPLLDVPPRSVSLTAAQAMGHSAVQLFVE
                     RAASALGRFSLTDETAPVVAEICRRLDGIPLAIELAAPRLKMLQPNGLLARLDDQLRL
                     LTTRSRTAVPRQQTLRAAIEWSYALLSEAERAILRNLGVF"
BASE COUNT          394 a          749 c          756 g          427 t
ORIGIN      
        1 tgaggccacc tgacccgcag cgacaccggc tcctaccgca attgcagcca acgcggctgt
       61 atccgcatcc cgccgctcgc catagagaaa cctgcattgg ccttcgctga agccggtctg
      121 ctggcacttg gccatgtatg cagcgcgacg ccccggctcg gttgcgatga cggtccaggc
      181 gatagcgatg gggaggatga aggcgccact gaatatgcag cccatgatga tgtggaaccg
      241 acgatccgag tgtcggttgg cagcggcccg ccctgtatgg tcgaaggctg acatcgcttc
      301 ctgagaatga gggttggtta acgtcgcccc agcatatctc gactccgacg cagctgatac
      361 ggcgcccagc ggtctgccac ggcccgcaac ccctcccggc cgatcagcag ggccaccaat
      421 cggccaggag atggtcaacc tgacttgccg gggctccagc gagggtctta cgggcggagc
      481 cattgcggcc ctgccatagc acgccgcctg ataccggctg tcggcggagg ttaggcatgc
      541 cggaggtagc caaggcgctg ggtgtcgggg tcgccagcca attgggccgc cagcaaccgg
      601 cggctcagca ccacctgata gagatcgccg atgcgcccag ggcgggggct gatggcgagc
      661 gacctattcg cccgaacagg cgctgtctct gccagtgcct gaggccaaga tgggtggtct
      721 tctgccggtc cgcttgccgg ccaggatgct taaggatcga acattcatct caagcctccg
      781 gccgcaacca tgctgatttt cgacggcagc cagtcgaggt gtattatcac agcgtgcgga
      841 agcgggacgc tctcccatgg ctgggcccac cgaaccgaac ccgactatta ggccaccgga
      901 tcggcggaag ctgatcgctg tgctctacgc cgatatggtt ggttacagtc gtttgatcgg
      961 actggacgat gcaggcaccc ttcaacgtct acgtgctttc cgtaccaacc tgatcgatcc
     1021 ggccatcgag gagcatggcg gcaggatcgt ccagacaggg ggagactccc ttctgatcgt
     1081 cttcgacagc atcgacgggg cgatgcgctg tgccctgaag gtgcaacagc gggtgcctgt
     1141 gctcgacagc gatcagccta ccgatcagtc catccgcttc cgtatcggca tcgacatcgg
     1201 tgatgcaatt gccgatggta ctgacttaca cggggacgcg gtgaacgtgg ttgcgcgtct
     1261 ccaggctgag tgcccgccgg gaggtgtctg cgtctctcgt tccgttcggg atcatgtgca
     1321 cggacgcctc ggcctggcat tcgaggcgct gggcccgctt catctgaaga acatcgcccg
     1381 gccggttgag gcattcgtcg tgaggctcga tgcgccgaag atgccacgtg gcgtcgggga
     1441 cgccggctcg caggtggaca ggccacctgc cgaacctgcg ccagcgtatc gtaacaatct
     1501 cccccagctc gcccatgcac tgattggacg ccagcgcgac gtggccgaga tcgaggcgtt
     1561 gctgtcgagg tgccgcttgg tgacgttggt cggtgccgcc ggcgtcggca aaactagcct
     1621 gtcgttgcag gtgggcgcca atctgttgtc gggcttcccg gacggcgtgt ggtttgtcga
     1681 gcttgcgccg ctggaccagg ccgaacttat cggcgaggcg gtcgccgccg tcttcggact
     1741 gccagtgcag ggggagcgac cgaccaccga cgcgatagcg gctttcttgc gatccaggcg
     1801 cgtgctcctc atccttgaca attgcgagca cgtaattgcg gcagccacca acctcgccaa
     1861 cgcacttctg aagacgtgcc cgggcgtatt cctacttgcc tccagccggg agccgctgtc
     1921 cgtggggggc gagcatgcct accccgtgcc tctgctggac gtgccgccgc gatcagtaag
     1981 cctcaccgcc gcccaggcga tgggacacag cgctgtgcaa ctcttcgtcg agcgcgccgc
     2041 ctcggcactt gggcgcttct cgttgaccga cgagaccgcc cccgtcgtcg cggagatctg
     2101 ccggaggctg gacggcattc cgctggcgat cgagctggcg gcaccacggc tgaagatgct
     2161 gcagcccaat gggttgcttg cgcgcctgga tgaccagctt cgcctcctca ccacgaggag
     2221 ccggacggct gtgccgcggc aacagacgct gcgcgcagcg atcgagtgga gctacgccct
     2281 gctctcggaa gccgagaggg ccatcctgcg caatctcggg gtcttc
//
LOCUS       DATGEM010000124         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_79280, whole genome shotgun sequence.
ACCESSION   DATGEM010000124 DATGEM010000000
VERSION     DATGEM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79280"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VKQ27_03900"
     CDS             complement(<1..847)
                     /locus_tag="VKQ27_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY88103.1"
                     /translation="MTDALILASIDAFLARDVEPFVHKLEHDDIYPEAIVARMRELGL
                     FGATITPNYGGLGLPCATYAEIVERIARVWMSLTGIFNSHLIMSAIVQRNGTEAQRRE
                     FLPRFASGELRGGLALTEPDCGTDLQAIRVTARRDGDTYVVNGTKTWISNGIYGSCFA
                     LLVKTDPAAEPRHRGMSMLLAEKGPGFTVSRKLEKLGYKGIDSAELVFQDYRVPADRL
                     VGGEEGRGLQHALSGLELGRINVAARGVGIAQAALDESLRYAQLRKTFGQPIAQHQAI
                     QLKLAD"
     gene            complement(844..1791)
                     /locus_tag="VKQ27_03905"
     CDS             complement(844..1791)
                     /locus_tag="VKQ27_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY88104.1"
                     /translation="MANRINRAVELLEQGQAIYYDGPHTGHVLTMAQGRDDAGTWADY
                     INVGMEHGAFDMTGLAHYMRGLVDGGPTRSGHRTPAIIVEAPVNGTDEPNVRFNAWQF
                     RQILGRGVHGILLCQAETVDAVRAFIESCRYPHHSEGVDPSLPTPVERMHGAQRPLHG
                     ATGKLGIGTRGRGSEATAAEIWGVSQQEYMQRCDPWPLSPGGELLLGVKLESPEGIAN
                     CEAICAVPGLGFAELGPGDLGLSLGYTTLQRDPYPPEMAEARARVFAACRRNGIAFLE
                     GCSPENIRGRLDEGVRVIAGHNPETARIGRAHQNRMMPA"
     gene            1868..3568
                     /locus_tag="VKQ27_03910"
     CDS             1868..3568
                     /locus_tag="VKQ27_03910"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88105.1"
                     /translation="MGMLALGAPDDDATISALNELAAAELAQRHFDSAVDCFRRILKV
                     RPAEPETLQSLGNALYDAGRFEEAIATYRQGVEAAPNSAGIYNNLGNALRAVGRLEEA
                     IEAYRSAVGLRPDHVLLYYNLGQALSLLDREDEAAESYRCALAINSGDPDALIGLGNT
                     LRKGKQPDVAEQHFQEALDHYLHALRVRPNDASALIGVGSALLGLFRHVEAVPYLERA
                     LAQRPEDNDTRIALGNALLGANRNAEALVQYREAEKHTTDTAALRLNQALALLTIGRW
                     TEGWELLESRFSVPQAFPGVDLPRDVQFWRGEDIAGKTILLQGEQGLGDTIHLVRYAP
                     LVAERGARVVLRVQPVLGKLIGGMRGVDTVLTGFDAAPEVDVVCPLMSLPLAFNTELA
                     TIPASVPYLAPPEPYRLLWRTLLGERRRPRIGVAWRGQQHLPHRSMPLAQLAPLLQRA
                     DYEFHGLQKEMSDADRAWLTANPLLIDHGEDLRDFADTAAVAEAMDLVISIDTSVAHL
                     AGALARPLCIMLPFAADFRWLLDRTDSPWYPTARLFRQPCTGDWNSVILAVTAALPTA
                     "
     gene            complement(3575..>5157)
                     /locus_tag="VKQ27_03915"
     CDS             complement(3575..>5157)
                     /locus_tag="VKQ27_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HLY88106.1"
                     /translation="QGRTEGMNQWKSAHAAATRARTLTDALDGADVFFGLSVKGAVTK
                     DMVRRMADKPIIFAMANPDPEITPEEAREASPHAIIATGRSDYPNQVNNVLGFPYIFR
                     GALDVRASTINDAMKIAAAEALANLAREDVPDEVHSASAGRRLTFGPEYIIPNAFDPR
                     LISWIAPAVARAAMDSGVARRPLIDLRRYARELSGRLDPTAGALDAIMESVRANPRRV
                     VFAEGEEEKVVRAAVAYRNAGYGRPVLIGREDRVKATMASLGLGMVEGIEIHNARLSH
                     NNRKYTDMLYHRLQRRGFLQRDCQRMVNQDRNIFAACMVATGDADAMITGLTRSAAVC
                     LDDIQHVLDPAPSEVAFGLTLMVGNRGKTIFIADSLVHFRPDAEQVAAIAIGAARAAR
                     HLGHEPRVALLSHSTFGNPPHDRALPMRDAVAILDQRKVDFEYDGEMSPDVALEPSIR
                     ALYPFCRLTADANVLLVPGLHSAHITARLAHHLSGGTTIGPLLMGMERPVQIVPMDAS
                     VSQMVDMACLAAYQAAPR"
BASE COUNT          839 a         1585 c         1806 g          927 t
ORIGIN      
        1 tatccgcgag cttcaattgg atcgcctgat gctgcgcgat cggctgtccg aaggtcttgc
       61 gcagctgggc gtagcggagc gactcgtcca gcgccgcctg cgcgatgcct acgccgcggg
      121 ccgcgacgtt gatacgcccc agctccagcc ctgacagcgc gtgctggagg ccgcggcctt
      181 cctctccacc gacaagccga tcggcaggga cacggtaatc ctggaacacc agctccgcgc
      241 tatcgatccc tttgtagcca agcttctcga gcttgcggct gacggtgaag ccggggcctt
      301 tttccgccag cagcatactc atgccgcgat ggcgcggttc ggcggccggg tcggtcttca
      361 ccagcagggc gaagcaggag ccgtatatac cgttgctgat ccatgtcttg gtgccgttca
      421 cgacataagt atcgccgtcg cgccgcgcgg tgacacggat cgcctgaaga tccgtgccgc
      481 aatcgggttc tgtcaaagcg aggccgccgc gaagctcacc cgaggcgaac cggggcagga
      541 attcgcgcct ctgtgcctcg gtgccgttgc gctgcacgat cgcggacatg atcagatggg
      601 agttgaaaat gccggtcagc gacatccaca cgcgggcgat gcgctcgact atctcggcgt
      661 aggtcgcgca cggaaggccg aggccgccat agttgggtgt gatcgtcgcg ccgaacaagc
      721 ccagttcgcg catgcgggcc acgatcgctt cggggtagat gtcgtcatgc tccagcttgt
      781 ggacgaacgg ttcgacgtcg cgggcgagga aggcatcgat ggaggcaagg atcagcgcat
      841 cagtcatgcg ggcatcatgc ggttctgatg ggcgcggccg atgcgggcgg tctcgggatt
      901 gtggccggcg atgacgcgca caccttcgtc cagtcggccg cggatgtttt ccggcgagca
      961 tccttccagg aaggcgatgc cgttgcgccg gcaggcggcg aagacacgtg cgcgcgcctc
     1021 cgccatctcc ggcggatagg gatcacgctg caatgttgtg tagccgagcg acaggccgag
     1081 atcgcccggt cccagctcgg cgaagcccaa tcctggtact gcgcagatcg cttcgcagtt
     1141 ggcgatccct tcgggacttt cgagcttgac gcccagcaac agctcgcccc cgggactcag
     1201 cggccatgga tcgcagcgct gcatatactc ctgttgggac acgccccaga tctccgctgc
     1261 tgtcgcctcc gagccgcgtc cgcgcgtgcc gataccgagc ttgcccgttg cgccatgtag
     1321 cgggcgttgc gcaccgtgca tgcgttcgac tggcgtcggc agtgacggat cgacaccttc
     1381 ggaatggtgc ggataacggc acgactcgat gaacgcacgc accgcatcca cggtttcggc
     1441 ctggcacagc aggatgccgt gcacaccgcg gccgaggatc tggcggaact gccatgcatt
     1501 gaaccgcacg ttcggttcat cggtgccgtt caccggcgct tcgacaatga tggccggcgt
     1561 ccgatggccg gaccgagtcg ggccgccatc gacaaggccg cgcatgtagt gcgcgaggcc
     1621 tgtcatatcg aacgcgccgt gttccatgcc gacattgata taatcggccc aggtgcccgc
     1681 atcgtcgcgg ccctgcgcca tagtcagcac gtggccggta tgcgggccat cgtagtagat
     1741 cgcctgccct tgctccagca attcgacggc gcgattgatg cggttggcca cggcgttcct
     1801 cctcaactcg tgcgccggac ggtagcaccc gccgacctat cgacaaaccg gcatctgggt
     1861 tatctggatg ggcatgctcg ccctcggtgc gccggacgac gacgccacga tcagcgcgtt
     1921 gaatgagctc gctgccgcgg aattggcgca acgccatttc gacagcgcgg ttgattgctt
     1981 tcgccgcata ctgaaggttc gtccagccga accggagaca ctgcaaagcc tgggcaacgc
     2041 cctgtatgat gccgggcgct tcgaggaagc gatcgccacg tatcgccagg gtgttgaggc
     2101 ggcaccgaac agcgccggca tctacaataa tcttggcaat gcgctgcgcg cggtgggccg
     2161 gttggaggag gcgatcgagg cgtaccgcag cgccgtcgga ctgcgtccgg atcatgtgct
     2221 gctgtattac aatctcggcc aggcactgtc gctgctggat cgcgaagatg aggcggcgga
     2281 aagctaccga tgcgcgctcg cgatcaattc gggcgatccc gatgcactga tcgggctggg
     2341 caacacgctg cgcaagggca agcagccgga cgtggcggag caacatttcc aggaggcctt
     2401 ggaccattac ctccatgcgc tgcgcgttcg ccccaacgat gcttccgcgc tgatcggcgt
     2461 tggcagcgcg cttctcggtc tgttccgcca tgtcgaggca gtgccgtatc tggaacgcgc
     2521 tctggcgcag cgtcccgagg acaacgatac aaggattgcg ctcggcaatg cattgcttgg
     2581 tgcgaaccgc aatgccgaag ctttggtgca gtaccgtgag gcggagaagc ataccaccga
     2641 taccgctgcc ctgcgcctga atcaggcgct tgcgttgctg acgattggta gatggacgga
     2701 agggtgggaa ttgctggagt cgcgattctc ggttccgcag gcgtttccgg gcgttgacct
     2761 gccgcgagat gtgcagttct ggcgcggcga agacatcgcg ggcaagacga tcctgctgca
     2821 gggagagcaa gggcttggcg acacgatcca tctggttcgt tacgcgccat tggtggcgga
     2881 acgtggcgca agagtggtgc tgcgcgtgca gcctgtgctc ggcaagttga taggcggtat
     2941 gcgcggcgtc gacacggtgc tgacgggatt cgatgccgca ccggaggtgg atgtcgtttg
     3001 cccattgatg agcctgccgc ttgccttcaa caccgaactc gcgacgatac cggcgagcgt
     3061 gccttatctc gccccgccag aaccgtatcg actgttatgg cggaccttgc tcggcgagcg
     3121 caggcggccg cgcattggcg tggcgtggcg cggtcagcag cacctgccgc atcgctcgat
     3181 gccgctcgcg caactcgcgc ccttgctgca acgcgcggac tatgagtttc acgggctgca
     3241 aaaagagatg tccgatgcgg accgggcgtg gctgacggcc aatccgctgc tgatcgacca
     3301 tggtgaggac ctgcgggact tcgctgatac cgcggccgtc gccgaggcga tggacctggt
     3361 gatctccatt gatacctcgg tcgcgcatct ggccggcgcg ctggcgcggc cgctctgcat
     3421 catgctgccg ttcgccgcgg atttccgctg gttgctcgat cgcaccgaca gcccttggta
     3481 tccgacggcg cgcctgttcc gacagccatg caccggcgac tggaatagcg tgatccttgc
     3541 ggtgacagcg gcgctgccga ccgcctgacc cccgctatcg gggcgccgcc tgatacgcgg
     3601 cgaggcaggc catatccacc atctggctga ccgatgcgtc catcggcacg atctggaccg
     3661 gccgctccat gcccatcagc agcggcccga tcgtggtgcc gccactcaga tggtgggcga
     3721 ggcgcgcggt gatgtgggcg gagtgaagac ccggcaccag cagcacgttg gcgtccgcgg
     3781 tcaggcggca gaacgggtac agggcgcgga tggatggttc gagggcgacg tcggggctca
     3841 tctcgccgtc gtactcgaag tccaccttgc gctgatccag gatcgccacc gcgtcgcgca
     3901 tcggcagcgc gcggtcgtgg ggcgggttgc cgaaggtgga atgcgacaga agggcgacgc
     3961 gtggctcgtg gccgagatgg cgtgcggcgc gggcggcacc gatggcgatg gcggccacct
     4021 gctcggcgtc gggtcggaaa tgcaccagcg agtcggcgat gaagatcgtc ttgccacgat
     4081 tgccgaccat gagggtgagg ccgaaggcga cctcgctggg ggcgggatcg agcacgtgct
     4141 ggatgtcatc gaggcagacc gcggcggagc gggtcaggcc ggtgatcatg gcgtcggcat
     4201 cgccggtcgc gaccatgcag gcggcgaaga tgttgcggtc ctggttcacc atgcgctggc
     4261 agtcacgctg caggaagccg cgacgctgca ggcgatggta gagcatgtcg gtgtatttac
     4321 gattgttgtg cgagaggcgg gcgttgtgga tctcgatgcc ttccaccatg ccgaggccga
     4381 ggctcgccat ggtggctttc acccgatcct cgcggccgat caggactggc cggccgtaac
     4441 cggcgttgcg ataggcgacg gcggcgcgga cgactttttc ctcctcgcct tcggcgaaga
     4501 ccacgcgccg gggattggcg cggacgcttt ccatgatggc gtcgagcgcg ccggcggtgg
     4561 gatcgagccg gcccgacagt tcgcgggcgt agcggcggag atcgatgagg gggcggcggg
     4621 cgacgccgct gtccatcgcg gcccgggcga cggcaggagc gatccaggag atcaggcgcg
     4681 gatcgaacgc gttggggatg atgtattcgg ggccgaaggt gaggcggcgg ccggcggagg
     4741 cggagtggac ctcatcgggg acgtcctcgc gcgccagatt ggcgagcgcc tcggcggcgg
     4801 cgatcttcat cgcgtcgttg atggtgctgg cgcggacgtc gagggcgccg cggaagatgt
     4861 aggggaaacc gagcacgttg ttgacctggt tcggatagtc gctgcggccg gtggcgatga
     4921 tcgcatgggg gctggcttcg cgggcttcct cgggcgtgat ctccgggtcg gggttggcca
     4981 ttgcgaagat gatcggcttg tcggccatgc ggcggaccat gtccttcgtc accgcgcctt
     5041 tcacggacag gccgaagaac acgtcggcgc catcgagtgc gtcggtcagc gtgcgggccc
     5101 tggtggcggc ggcgtgggcc gacttccact gattcatgcc ctcggtgcgg ccctggt
//
LOCUS       DATGEM010000125         7836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_421361, whole genome shotgun sequence.
ACCESSION   DATGEM010000125 DATGEM010000000
VERSION     DATGEM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421361"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..6437
                     /locus_tag="VKQ27_03920"
     CDS             159..6437
                     /locus_tag="VKQ27_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLY88107.1"
                     /translation="MPRKSRKAASGTAAQLAMRRPSVSLSRAPGAESLKRLVDTGMRL
                     NALRSAEELQERLIDEIIELSCADRALLVFSDADGLRTVGSRLPAGEDAAALLKVITP
                     WLEESSRTRKARLRYGPEGAQIGDQRSCLVLPLIAKHELLGYLYADIEGTRGRFQDAD
                     RDLLATLASQAAVALANIRLADELQRKLAERDTQLEQRTSELAVINSIQQGMAAQLGF
                     EAIIDMVGDRLREVLGFGDVQIVLWDAPTGTAHVPYAYERGVRIQVPPRRPNPDGPMY
                     KALQAKRPVIANNRAEMTAWGLRTVEGTRPSLATAITPIFSGGRMIGSIVLENHEREN
                     AFGEAEVRLLSTIAASMGVALENASLFNETREALERQTATADILRVISESHTDVQPVF
                     DAIVASAYQLLAANSFALVLLRTGDGFRLVAMYQEGTGVGLPPNADFNPIDPAANFPS
                     RVFVSGEMLHLPDWSTIELPPHEVRVRASLKVEASLMMPMLRGDECIGVLAVGRHAPQ
                     AFNAQEIALMRSFVDQAMIAIENVRLFNETKEALERQTATAEVLRVISNSVADAQPVF
                     DKITESCHSLFNGAQVGINLVRPDGLIDLAAYVGPDEKKLRAMYPFPMDNESGTALVI
                     RERRAIHWPDALAGADVPPAVRRGAERIGARSSIFAPLVWEDRGIGAIFVARNTVSPF
                     SDHEISLLKTFADQAAIASQNARMFNETKEALERQTATANVLKAISRSTFDLTAVLET
                     LISTAARLCRAWMGVIFRIDGDICRPAGLFGATPALIEHLEAHPISLRDQDSVTSRAV
                     AAGHAVQVEDATDQLKYGRGDVQQVGGYRTLLAVPILREGVAVGVLTLGRAEVQPYNE
                     KEIELVTSFADQAAIAMENVRLFNETKEALEQQTATAEVLQVISKSTFDLVPVFDALV
                     KNAAYLCGAKTGAIFRRDGDLLHAAAWEGASESMVEFLRSYRIPLDRRTATGRAASEG
                     RTIQVLDAMNDSEYSFGGQSIENYRTIIGVPLMRDGQAIGVFTLWRHHVEAFSPRQIA
                     LVETFADQAVIAIENVRLFNETKEALEQQTATADVLEVISASVADTAPVFDKILESCK
                     KLFDSSEQGIVLVNPEGHIALAAHHGSALPTLREIFGGAKIPAEPYLPGILRGEPLHF
                     VDTLDPEVHWTVRSVAEQLKIGHYSQVLAPMTWEGQAVGFLYVIRKPATGFANKEIAL
                     LETFADQAVIAIQNARLFNETKEALEQQTATSEVLQVISGSVSDTKPVFERILQSTTR
                     LFNCTGAAIFLAPGDAQLHFAAGTGAAVANIAALYPQPLEQTSASLVISERRQMYFPD
                     VMNGEDVPPSLRRAADVQGNFSTVLTPMLWNNEGIGLIAIRREPNATFNDKELNLLKT
                     FADQAVIAIQNARLFNETKEALEQQTATAEVLQVISSSVADAAPVFDKILDSCRHLFA
                     IEDLGIFLLDDDNLVRVAAYRGSMFDVMVRTFPRPLDQTMTAQVIRTLRPVHVSDTAA
                     MTDAPAAMRSVVELKGGASVVYVPMLWEDRGIGSIMIGRQPPKPFSDKEIALLKTFAD
                     QAVIAIQNARLFKQTQEARAAAEAANEAKSSFLATMSHEIRTPMNAVIGMSGLLLDTS
                     LDDEQRDYAATIRDSGDTLLTIINDILDFSKIEAGRMDIEAQPFDLRDCVESALDLVS
                     ARATEKHLDTAYLFEGDVPSGIRGDVTRVRQIILNLLANAVKFTEQGEVVLTVIASPV
                     AAGEVELNFAVRDTGIGLSTEGMGRLFQSFSQADSSTTRKYGGTGLGLAISKQLAELM
                     GGRMWATSDGIGKGATFHFTIRAPLADLPPQNRREFIGTQPELQGRRVLVVDDNATNR
                     RVLELQMGKWGMVPRATELPAEALRWVEDGEDLDLAILDMHMPGMDGLQLAQRIRASR
                     PDLPLVLFTSLGRREAGDTAGLFSAHLAKPVHQSQLFDTLVSLLTQALAPKGIKAAKP
                     RSDPEMAARHPLRILLAEDNVVNQKLALRILTQMGYRADLASNGVEAVESVVRQTYDV
                     VLMDVQMPEMDGMEASRRINAQGQNSKRPRIVAMTANAMQGDREMCLAAGMDDYITKP
                     IRVDQLVDALMRVPARQD"
     gene            6443..6808
                     /locus_tag="VKQ27_03925"
     CDS             6443..6808
                     /locus_tag="VKQ27_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLY88108.1"
                     /translation="MASEEDMDESEIDQAVFADLQDTAGAEFVAELVDTFLEEAPGML
                     AELHSARADADADRFRRAAHSLKSNCNTFGALKLAALARELELKGLDADPARDAGGLA
                     ALEVEYARAAGALKALRHG"
     gene            6801..>7836
                     /locus_tag="VKQ27_03930"
     CDS             6801..>7836
                     /locus_tag="VKQ27_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY88109.1"
                     /translation="MADPAARVLVVDDNKVNRLLLARSVEMQGHRAALAENGRVALEM
                     LRREPFDLLLLDIEMPEMDGFAVLERLKADPQLRDLPVIVTSSVEGLDNIVRCIGLGA
                     EDYLPKPVNPVLLKARIGASLEKKRLRDQQKELVRRFATSEVAQDLQQSGFALGGKRV
                     RGSVMFADIRGFTALVESQSPEETIELLNTYYTLMFDAISGRGGVINQMIGDGLMAIF
                     GAPLPLADSSGSAVHAALDMIEMIELFNVDRVAAGKPAIRIGVGIATGEMVAGYTGTQ
                     QRATYTCIGDTVNLAARLEAHTKVAACPILIDEAARVGLRGRFPVNALGSVAFKGKAL
                     EVEVFSVDVPG"
BASE COUNT         1478 a         2453 c         2588 g         1317 t
ORIGIN      
        1 ggtcagttca agctgaaacc cgtagacggc cagcgccatg caacttggct gcgaggagta
       61 cctgacagac gggttgcacg gcttcgccac tgaaccgatc cgcgcgcatg cattttccga
      121 tgcgctgcat gagtgggctg ccagggagga gaccagagat gccacgcaag agcaggaagg
      181 cggcttcggg cacagcggcg cagctggcca tgcgccggcc ttcggtttcg ttgtccaggg
      241 ctcctggtgc cgagtcgctc aagcgcctgg tcgacaccgg catgcggctc aacgcgctgc
      301 gcagcgccga agaactgcaa gagcgcctga tcgatgaaat catcgagctc agctgcgccg
      361 atcgcgcgct gctggtcttc agcgatgctg atggcttacg taccgtcggc tcgcggttgc
      421 ccgcaggtga ggatgcggcc gcactgctga aagtcatcac gccctggctc gaagaatcca
      481 gccgcacccg caaagcgcgc ctgcgttacg ggcccgaggg cgcgcagatc ggggatcaac
      541 gctcctgcct cgtcttgccg ctgatcgcga agcatgaact gctcggctac ctctacgccg
      601 acatcgaggg cacgcgcggc cgcttccagg acgccgaccg cgacctgctt gcgacgctgg
      661 cgtcacaagc agcggtggca ctggccaaca tccgcctcgc cgacgaactg cagcgcaaac
      721 tggccgagcg cgacacccag ctggagcagc gcaccagcga actcgcagtg atcaacagca
      781 tccagcaggg catggcggcg caactcggct tcgaggccat catcgacatg gtcggtgacc
      841 ggttgcgcga ggtgctgggg ttcggcgacg tccagatcgt gctttgggat gcgccgacag
      901 gcaccgcgca cgtgccgtac gcatacgagc gcggcgtgcg cattcaggtg ccgccgaggc
      961 ggccgaaccc cgacgggccg atgtacaagg cgctgcaagc gaagcgcccg gtgatcgcga
     1021 acaaccgcgc cgaaatgacc gcctggggcc tgcgcaccgt ggaaggcacg cgcccgagcc
     1081 tggccacggc catcacgccg atcttttcgg gcggccggat gatcggctcc atcgtcctag
     1141 agaaccatga gcgcgagaac gccttcggcg aggccgaggt gcggctgctg agcactatcg
     1201 ccgcgagcat gggcgtggcg ctcgagaacg ccagcctgtt caacgaaacc agggaggcgc
     1261 tggagcggca gacagcgacc gcggacatcc tccgggtcat cagcgaatca cataccgacg
     1321 tgcagccggt gttcgatgcc atcgttgcat cggcgtatca gctgctggcg gcgaattcgt
     1381 ttgccctggt gctgctgcgc acgggcgacg gatttcgcct ggtcgccatg taccaggagg
     1441 gcacgggcgt gggcctgcct ccgaacgccg atttcaatcc gatcgatccc gccgctaatt
     1501 ttccctcgcg ggtgttcgtg agcggggaga tgctgcacct gccggattgg tcgaccatcg
     1561 agttgccgcc tcatgaggtg cgtgtgcgcg cgagcctgaa ggtggaagcg agcctgatga
     1621 tgccgatgct tcgcggcgac gaatgcatcg gtgtcctggc cgtcgggcgc catgcgccgc
     1681 aggccttcaa tgcccaggaa attgccttga tgcgatcctt cgtcgaccag gccatgatcg
     1741 cgatcgagaa tgtgcgcctg ttcaacgaaa ccaaggaggc gttggagcgg cagacggcaa
     1801 ccgccgaggt gttgcgggtc atcagcaatt cggtcgccga cgcgcagccg gtcttcgaca
     1861 agatcaccga aagctgccac agtctcttca atggcgcgca ggtcggaatc aatctggtgc
     1921 gccctgacgg attgatcgat ctggccgcct atgttggccc ggacgaaaag aaactacggg
     1981 cgatgtatcc cttccctatg gataacgagt ccggcaccgc gttggtcatt cgggagcgcc
     2041 gtgcgatcca ctggccagat gcgctcgctg gagccgatgt gccgcccgcg gtgagacggg
     2101 gcgccgaacg catcggcgcc cgctcgagta tctttgcgcc gctggtctgg gaggaccggg
     2161 gtatcggcgc gatcttcgtg gccaggaaca cggtctctcc gttctcggac catgagatct
     2221 cgttgctcaa gaccttcgcc gaccaggcgg ccatcgcaag tcagaacgca cggatgttca
     2281 acgagaccaa agaggcgctg gaacggcaga ctgcgaccgc caatgtgctc aaggcgatca
     2341 gccgctcgac tttcgacctg acggcggtgc ttgaaacgct gatcagcact gcagcccgcc
     2401 tgtgtcgtgc gtggatgggt gtgattttca ggatcgatgg cgacatatgc cgtcccgccg
     2461 gcttgtttgg cgccacgccg gccctgatcg aacatctgga ggcccatccc atatcgctgc
     2521 gcgaccagga ttccgtcaca tcgcgtgccg tagccgccgg gcacgccgtg caggtcgagg
     2581 atgcaaccga tcaactgaag tacgggcgcg gggatgtcca gcaggtcggc ggttaccgaa
     2641 cgctgctggc ggtgccgatc ctgcgcgagg gcgttgcagt cggcgtgctg acgctcggcc
     2701 gcgcagaggt ccagccctac aacgagaagg aaatcgaact cgtcacctcg tttgccgacc
     2761 aggctgcgat cgcgatggaa aacgtgcgcc tgttcaacga gacgaaggaa gcgctggagc
     2821 agcaaaccgc cacggcggag gtcttgcagg tcatctcgaa atccaccttt gatctggttc
     2881 ccgtcttcga tgccctcgtc aagaacgcag cctacctgtg cggggccaag accggcgcaa
     2941 tattccggcg cgacggggat ctgttgcacg cggccgcctg ggaaggggcc agcgagtcga
     3001 tggtggaatt ccttcgcagc tacaggattc cactggaccg tcgaaccgcg actggtcgcg
     3061 cggccagcga gggccgcaca attcaggtcc tggacgcgat gaacgattcc gagtacagct
     3121 tcggcggtca gtcgatcgag aactaccgga caatcatcgg tgtgccgctg atgcgcgacg
     3181 gacaggcgat cggagtcttt acgctctggc gccaccacgt ggaggccttc tcgccacgcc
     3241 agattgcgct cgtcgagacc ttcgcggacc aggcggtgat cgcgatcgag aacgtgcggc
     3301 tgttcaacga gaccaaggag gccctggagc agcagaccgc gaccgccgac gtgctggagg
     3361 taatcagcgc ttcagtcgcc gacaccgcgc cggtgttcga caagatcctg gaaagctgca
     3421 agaagctgtt cgacagctcc gagcagggca tcgtgctcgt caacccggag ggccatattg
     3481 cgctggccgc ccaccacggc tcggcgctgc cgactctgcg ggaaatcttc ggtggcgcga
     3541 agatcccggc cgagccgtac ttgccgggta tcctgcgtgg cgaacccctg cacttcgtgg
     3601 atacactcga tcccgaagtc cactggaccg tgcggtccgt cgcggagcag ttaaaaatcg
     3661 gccactattc gcaggttctc gccccgatga cctgggaagg acaggcggtg gggttcctct
     3721 atgtcatccg aaaaccggcc acgggcttcg cgaacaagga aatcgctctg ctggagacct
     3781 ttgccgacca ggctgtgatc gcgatccaga acgcgcgcct gttcaatgag accaaggagg
     3841 cgctggagca acagaccgcc acttcggaag tgctgcaggt gatcagcggt tccgtgtcgg
     3901 ataccaaacc ggtgttcgag cgtatcctgc agagcacgac acggctcttc aattgcacgg
     3961 gagcggcgat cttcctcgcc cccggcgatg cccaattgca ttttgccgcc gggacgggcg
     4021 ccgccgtggc caacatcgca gcgttgtatc cccagccgct ggagcagacc tccgcaagcc
     4081 tcgtgatcag cgagcgccga caaatgtatt tcccggatgt gatgaacggc gaggacgtgc
     4141 cgccgagtct gcgccgggcg gctgacgtgc agggcaactt ctcgaccgtg ctgacgccga
     4201 tgctctggaa taacgagggt atcggcttga tcgcgatcag gcgcgaaccg aacgccacct
     4261 tcaacgacaa ggaactcaac ctgctcaaga ccttcgccga ccaggcggtg atcgcgatcc
     4321 agaacgcgcg gctgttcaac gagaccaagg aggcgctgga gcagcagacc gccaccgccg
     4381 aggtgctgca ggtgatcagc agctcggtgg ccgacgccgc accggtgttc gacaagatcc
     4441 tcgacagctg ccggcacctg ttcgccatcg aggatctggg catcttcctg ctcgacgatg
     4501 acaatctggt gcgtgtggcc gcgtatcgcg ggtcgatgtt cgacgtcatg gtacgcacct
     4561 tccccaggcc gctcgatcaa accatgacag cgcaagtcat tcgcactctg cgtccggttc
     4621 acgtgtccga cactgccgca atgaccgatg cgccggccgc aatgcgcagc gtggtcgaac
     4681 tcaaaggcgg tgcctcggtc gtctacgtgc cgatgctgtg ggaggaccgc ggaatcggct
     4741 cgatcatgat cgggcgccag ccacccaagc cgttctccga caaggagatc gcgctgctca
     4801 agaccttcgc tgaccaggca gtgatcgcga tccagaacgc gcggctgttc aagcagaccc
     4861 aggaagcccg cgcagcggcc gaggcagcga acgaagccaa gagttcgttc ctggcgacca
     4921 tgagccacga gatccgcacg ccgatgaatg cggtcatcgg catgagcggg ctgctgctcg
     4981 acacgtcgct cgatgacgag cagcgcgact atgcggccac catccgcgat agcggcgaca
     5041 cgctgctgac catcatcaat gacatcctcg acttctccaa gatcgaagcc ggacgcatgg
     5101 acatcgaggc gcagcctttc gacctgcgcg attgcgtgga atcggcactc gacctggtca
     5161 gtgcccgcgc taccgaaaag catttggaca ccgcatacct gttcgagggc gatgtgccgt
     5221 ccggcattcg cggcgacgta acgagggtgc ggcagatcat cctcaacctg cttgccaatg
     5281 cggtgaagtt caccgagcaa ggcgaggtgg tgctcacggt catcgcatca cccgtggcgg
     5341 ccggcgaggt cgagctgaac ttcgccgtgc gtgacaccgg catcgggctg tcgacggagg
     5401 gcatgggacg gctgttccag tcgttctctc aggcggattc gtcgaccacg cgcaagtacg
     5461 gcggcacagg cctggggctc gccatcagca agcagctggc cgagctcatg ggtgggcgca
     5521 tgtgggccac cagcgacggg atcggcaagg gggcgacgtt ccacttcacg atccgcgcgc
     5581 cgctggcgga tttgccgccg cagaaccggc gcgagttcat cggcacccag cccgaactgc
     5641 agggaaggcg tgtgctagtg gtcgatgata acgccaccaa ccggcgtgtg ctcgaattgc
     5701 agatgggcaa gtggggcatg gtgccgcgcg ccaccgagtt gccggccgag gcgctgcgct
     5761 gggtcgagga cggagaagac ttggacctcg caatcctgga tatgcacatg cccgggatgg
     5821 atggtttgca gctcgcgcaa cgcatccgcg ccagccggcc ggacctgccg ctggtgctct
     5881 tcacctcgct cggcaggcgc gaggccggtg ataccgcagg cctgttcagc gcccacctgg
     5941 cgaagccggt gcaccagtcg cagctgttcg acacgttggt cagcctgctc acgcaagcct
     6001 tggcgcccaa agggatcaag gccgccaagc ccaggtcgga tcccgaaatg gcggcgcgcc
     6061 acccgttgcg catcttgctc gcggaagaca acgtcgtgaa ccagaaactc gcgctgcgca
     6121 tcctcacgca gatgggctat cgtgccgacc tggcctccaa cggggtcgag gcggtggagt
     6181 cggtggtgcg gcagacctac gatgtggtgc tgatggacgt gcagatgccc gagatggacg
     6241 gcatggaagc gtcgcgccgc atcaacgcgc aaggacagaa cagcaagcgc ccgcgtatcg
     6301 tcgcaatgac ggccaacgct atgcagggcg accgcgagat gtgcctggcc gcgggcatgg
     6361 atgactacat caccaaaccg atacgggtgg accagttggt cgacgcgtta atgcgcgtac
     6421 cggcgcgcca ggattgatca gaatggcgag tgaggaggat atggacgaat cggagatcga
     6481 ccaggctgtc ttcgcggatc tgcaggatac ggcgggtgca gagtttgtcg ccgaattggt
     6541 ggataccttc ctcgaagagg cgccgggcat gctggccgag ctgcacagcg cgcgcgctga
     6601 cgccgatgct gaccgtttcc gccgcgcggc gcactcgctc aagtccaact gcaacacctt
     6661 cggcgcgctg aagctcgcag cactggcgcg cgaactggaa ctcaagggcc tggacgccga
     6721 cccggcacgg gacgctggcg gactggcggc tctggaggtg gagtacgcgc gtgccgctgg
     6781 cgcattgaag gccctgcgcc atggctgacc cggccgcacg cgtgctggtg gtcgacgaca
     6841 acaaggtcaa ccgcctgctg ctggcgcgca gtgtggaaat gcaaggccac cgcgccgcac
     6901 tggccgagaa cggccgcgtc gcgctcgaga tgctgcggcg cgaaccgttc gacttgctgc
     6961 tgctcgatat cgagatgccc gaaatggacg gctttgcggt gctcgagcga ttgaaggccg
     7021 acccgcagtt gcgcgacctg ccggtaatcg tgacctcgtc ggtcgaggga ctggacaaca
     7081 tcgtgcgctg cattggcttg ggcgccgagg attacctgcc caagccggtg aacccggtgc
     7141 tgttgaaggc gcggatcggc gccagtctgg agaagaagcg cctgcgcgac cagcagaaag
     7201 agttggtgcg ccgattcgcc acctccgagg tggcgcagga cctgcagcaa tcggggttcg
     7261 cgctgggcgg caagcgcgtg cgcggctcgg tcatgttcgc cgacatccgc ggcttcacgg
     7321 cattggtgga atcgcaatct cccgaggaaa ccatcgaact gctgaatacc tattacacgc
     7381 tgatgttcga cgccatcagc ggccgtgggg gcgtcatcaa ccagatgatc ggcgacgggc
     7441 tgatggccat cttcggggca ccgctgccgc tggctgacag tagcggcagc gcggtgcacg
     7501 cggcgctcga catgatcgaa atgatcgagt tattcaatgt cgaccgtgtc gcagcgggca
     7561 agccggcgat tcgcatcggc gtggggatcg ccaccggcga gatggtggca ggctacaccg
     7621 gtacccagca acgcgcgact tacacgtgca tcggcgacac ggtcaacctg gcggcgcgcc
     7681 tggaggccca caccaaggtg gccgcatgcc ctatcctgat cgatgaggcc gcacgcgtgg
     7741 gcctgagggg gcgtttcccg gtcaatgcgc tgggctcggt ggccttcaaa ggcaaggcac
     7801 tcgaggtgga agttttttca gtggatgtcc cgggtc
//
LOCUS       DATGEM010000126         7096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1677530, whole genome shotgun sequence.
ACCESSION   DATGEM010000126 DATGEM010000000
VERSION     DATGEM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677530"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VKQ27_03935"
     CDS             complement(<1..666)
                     /locus_tag="VKQ27_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY88110.1"
                     /translation="MDATPPSPLVQVRDLHLTVPSAAGPVNILRGIDLDIGAGEAMGI
                     VGPSGSGKTSLLMVLAGLERATSGSILLAGRPVTGLDEDALARLRREAIGIVFQAFHL
                     IPSMTALENVAVSMELAGARNALARAKDALAAVGLAHRLAHLPGQLSGGEQQRVALAR
                     AFAPRPRLLLADEPTGNLDQATGEAVMELLFRLRRDTGATLMLVTHDVALSARCSRCI
                     HMAD"
     gene            665..1318
                     /locus_tag="VKQ27_03940"
     CDS             665..1318
                     /locus_tag="VKQ27_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HLY88111.1"
                     /translation="MAAAYGVCGRWRKAVLALILAAALMPGTQAAPVRLLVLGDSLSA
                     GYGLAHADGFEAQLSGALHAQGRDVQIVDGAVSGDTTAGGLARLDWSLGDGADAAIVE
                     LGANDGLRGVDPKETEKNLTAILDKLAAKHIPVLLTGMYAMPNLGADYAAAFRAVFDR
                     LGERPGVLYDPFFLDGVAEVPSLNQSDGMHPNPEGVKHIVMRLLSLVDKLLAEVPPA"
     gene            1315..1851
                     /gene="thpR"
                     /locus_tag="VKQ27_03945"
     CDS             1315..1851
                     /gene="thpR"
                     /locus_tag="VKQ27_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HLY88112.1"
                     /translation="MRLFVGLDLPWELRERVSILAGAGIPGARWVPPENYHVTLRFIG
                     EAPRYLAEEIDHALAALKAPVFRLTLAGIGTFAKGGRVQSLWVGVERTAELDRLKSKV
                     ETALQRCGLDPERRRFQPHLTLARLDYAAEVKLAGFVQAHNLFRAEPVMVEHFTLFSS
                     LLGKEQPVYTAEVEYGLG"
     gene            complement(1937..2812)
                     /locus_tag="VKQ27_03950"
     CDS             complement(1937..2812)
                     /locus_tag="VKQ27_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY88113.1"
                     /translation="MNAPSNAQYRYIQTHRIAGSGGAIVEGVDLSEDLPDDVLAEIRA
                     ALLDNCVIFFRDQKLTPEQQLNFASRWGEIHLHPFMQGMKDYPEILEIIKVPEDKKNF
                     GGSWHTDQMFSPQPAMGTMLYAVEVPSAGGDTMFTNQYLAYESLSDGMKALCDQLHTV
                     CVGDNFKQHGGQSRAMRYERSMTHMKVKDPGNVQTTSVHPLVRTHPETGRKALYIGGH
                     VQHFDGMTDEESEPLISYLKQHSIRPEFVYRFRWETGSLAFWDNRCTQHFAINDYPAE
                     TRIMRRITICGDTPF"
     gene            3070..3600
                     /locus_tag="VKQ27_03955"
     CDS             3070..3600
                     /locus_tag="VKQ27_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY88114.1"
                     /translation="MLALPHLDAAYNLARWLAGNTTDAEDVVQDAYLRAYRYFDAFQG
                     GNFRVWLLTIVRNAFMTWVKENRSGRMVFVPDTPLAESADTEEAAWGSKPRDPEALLM
                     DSIDSQTLSRLMEKLPSDYREVLLLREVEDLSYKEIADVTGTPMGTVMSRLSRARLAL
                     RKLWLEHAELENADGM"
     gene            3614..4348
                     /locus_tag="VKQ27_03960"
     CDS             3614..4348
                     /locus_tag="VKQ27_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HLY88115.1"
                     /translation="MLSPLVDSALGPFTRWRVRRHVAGCTDCADKLEELRAMQSAIRT
                     KLTFHRAPPGLAARIGAALPREAAPAPSRPWFRMPAFSFGSTGLAGALAGVVLTLLVV
                     GGQPQSDLLQDVIGSHIRSLQADHLTDVLTSDQHTVKPWLSARLDVSPAVKELKDQGF
                     PLVGGREDYVDGHPAAVVVYRHDKHVINLFAWASPGRPEEAFRDESRQGFNIVTWRRG
                     GITYYAVSDVEADQLADFAKLVAEGT"
     gene            complement(4390..4803)
                     /locus_tag="VKQ27_03965"
     CDS             complement(4390..4803)
                     /locus_tag="VKQ27_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88116.1"
                     /translation="MKMINTLAAGALMLAATFGAAAAQSLPEGRVYVFHSTAQGACPA
                     LDWDIVVRANNTLSGTIAWDDMKSIAYATGGYDMVKRTFKMSAQEIRGHGRTATVDGQ
                     IRQDGWLIANIHGPKVTCLGIAVPWYTPPAGAGAG"
     gene            4948..5445
                     /locus_tag="VKQ27_03970"
     CDS             4948..5445
                     /locus_tag="VKQ27_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88117.1"
                     /translation="MLRHCLSVALLLLLLPPRAHADPAADAAQCRQAAGSYLVGTVTT
                     GPSFRGGKPLHGVYLSHTHLTLLGDDGTSYDVAIDNVFANGYRRNQAAVPPPLNSIRT
                     GDRLELCGKRYSGGDVGIDWVHTNCGEAPSHDKPDGWVRKLDANGTADGNMEASQAFC
                     SLWPN"
     gene            complement(5482..6036)
                     /locus_tag="VKQ27_03975"
     CDS             complement(5482..6036)
                     /locus_tag="VKQ27_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88118.1"
                     /translation="MLSRIVVMVVCVSLAGCAAATPPPDTAQLPFAAFGTMDNDVGAA
                     NIASSAFASPARTANNPVDGARACAAIDYLAGQLSSPRWLDLSPLTKQEMLRARGDVR
                     AVLGVPLGVPAQMVVGALLQFAAAWQAGNQQAALQFLVPPAFLNGPQQTLQVLRNLPY
                     IPTANRASIDAANQMLPGGDNARR"
     gene            complement(6104..6640)
                     /locus_tag="VKQ27_03980"
     CDS             complement(6104..6640)
                     /locus_tag="VKQ27_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLY88119.1"
                     /translation="MPDGPIYSLNGITPKIAPDAFVAPTAAVIGDVEVGSESGIWFHC
                     LVRGDMNFIHIGARTNIQDGAIIHVDSGAFSTTIGDDVTVGHNAVIHACTLKDRAFVG
                     IAATVLDGAVIEEGGMLGAGGLLTPGKVIGRNELWVGSPARLVRMMSDEERRKFDLNA
                     EVYRELAAQFRAGLRSAT"
     gene            complement(6633..>7096)
                     /locus_tag="VKQ27_03985"
     CDS             complement(6633..>7096)
                     /locus_tag="VKQ27_03985"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629761.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLY88120.1"
                     /translation="EQRGGPAFELYALKLEHKHLPETQLYSGLSRRPIFAPSVGHYRQ
                     GMLVSVPLHLDTLNGRPSGADLQTVLEARYRGCDYVRVVAPSADGKLEPEGLNYTNLL
                     ELSVYANEKQRQAVLVARLDNLGKGASGAAVQNMRLMLGLSVSAQPALAHA"
BASE COUNT         1275 a         2298 c         2243 g         1280 t
ORIGIN      
        1 gtcggccatg tggatgcagc gcgaacaccg cgccgacagg gccacgtcgt gggtgaccag
       61 cataagtgtc gcgccagtgt cgcgacgcag ccggaacagc agctccatca ccgcttcacc
      121 cgtcgcctgg tccaaattgc cggtgggttc atcggccagc agcaggcgtg ggcgcggggc
      181 gaacgcgcgg gcgagcgcca cccgctgctg ctcaccgccg gaaagctgac cgggcagatg
      241 ggccaggcga tgggccaggc ccaccgccgc cagcgcatcc ttcgcacgtg ccagcgcgtt
      301 gcgcgcgcca gccagctcca tcgacacggc cacgttctcg agcgcggtca tgctggggat
      361 caggtggaag gcctggaaca cgatgccaat cgcctcccgc cgcaatctgg cgagcgcatc
      421 ctcatccagc ccggtcaccg gacggccggc cagcaggatg ctgccggagg tggcgcgctc
      481 caggcccgcc agcaccatca gcaggctggt cttaccggag ccggatgggc cgacgatgcc
      541 cattgcctcg cccgcaccta tatccaggtc gatccctcgg aggatgttga ccggcccggc
      601 agcggacggc acggtcaggt gcaggtccct gacctggacc agcggagacg gcggagtggc
      661 atccatggcg gcagcatatg gcgtctgcgg ccgatggcgg aaagccgtgt tggcgctgat
      721 tctggcggcg gcactgatgc ccgggactca ggcggcgcca gtgcggctac tggtgctggg
      781 cgattcgctt tccgccggct acggactggc gcacgctgat ggattcgaag cgcaactgtc
      841 cggtgcgctg catgcacagg gccgcgacgt ccagattgtc gatggcgcgg tctcgggtga
      901 cacgacggcg ggcggcctgg cgcgattgga ttggtcattg ggcgacgggg cggatgccgc
      961 gatcgtcgaa ctcggggcga atgacgggct gcgaggcgtc gacccgaagg agaccgagaa
     1021 gaacctgacc gctatcctgg ataaactggc cgcgaagcat atcccggtgc tgctgaccgg
     1081 catgtatgcc atgccgaacc tgggcgccga ttatgccgcg gccttccgcg ccgtgtttga
     1141 tcgactgggg gagcggccgg gcgtcctgta cgacccgttc ttcctggatg gcgtagcaga
     1201 ggtaccgtcg ctcaaccagt cggacgggat gcatcccaac ccggaaggcg tgaagcacat
     1261 cgtgatgcgg ctgttatctc tggtggacaa gctgcttgcg gaggtgccac cggcatgagg
     1321 ctgttcgttg gcctggacct gccctgggaa ctgcgcgagc gagtttccat actcgcaggt
     1381 gccggcattc caggcgctcg ttgggtaccg cctgaaaact accacgtgac gctgcgcttt
     1441 atcggcgagg ccccacgcta cctcgccgag gaaatcgacc acgcccttgc agctctgaaa
     1501 gcgccggtgt tccggctgac gctggctggg attggcacgt tcgccaaagg cggacgcgtg
     1561 caatcgctgt gggttggcgt agagcggacc gctgaactgg atcggctgaa aagcaaggtg
     1621 gagacggcac tgcagcgctg cggccttgat ccggagcgac ggcggtttca gccacacctt
     1681 acattggcgc ggctggacta tgcggcggag gtgaagctgg cggggttcgt gcaagcgcac
     1741 aacctgttcc gcgccgagcc cgtgatggtc gagcacttca cgctgttcag ctcgctgctg
     1801 gggaaggagc agcctgtgta tacagcggaa gtggagtatg ggctgggatg aggactaccc
     1861 cctcgtcacc cggcccccgg gtcaagcccg gggcaggcct ctcggcccgc gagggggcga
     1921 agaggggaag agaaagctag aacggggtgt caccgcagat cgtgatgcgc cgcatgatgc
     1981 gggtctctgc cggatagtcg ttgatcgcaa agtgctgcgt gcagcgattg tcccagaacg
     2041 cgagtgatcc cgtctcccaa cggaacctgt agacaaactc cggtcggatc gagtgctgtt
     2101 tcagatagct gatcagaggc tcgctctctt cgtcggtcat gccgtcgaag tgctgcacat
     2161 gtccaccgat atacagcgcc ttgcgcccgg tctcggggtg agtgcgcacc agcggatgca
     2221 ctgacgtcgt ttgcacgttg ccagggtcct tcaccttcat gtgcgtcatc gagcgctcat
     2281 accgcattgc gcgcgactga cccccatgct gcttgaaatt gtcgccgacg cagactgtgt
     2341 gaagctggtc acacagtgcc ttcatgccgt ccgagaggct ttcgtaagcg aggtactgat
     2401 tggtgaacat cgtatcgccg ccggccgacg gcacctctac cgcgtagagc atcgtgccca
     2461 ttgccggctg cggtgagaac atctggtcgg tgtgccagga gccaccaaaa ttcttcttgt
     2521 cctcggggac tttgatgatc tccaggatct ctggatagtc cttcatcccc tgcatgaatg
     2581 gatgcaggtg gatctcgccc caacggctcg caaagttgag ctgttgttcc ggcgtcagct
     2641 tctggtctcg gaagaagatc acgcagttgt cgagcagcgc ggcacggatt tcggcgagga
     2701 catcgtcggg caggtcctcc gagagatcga cgccttccac gatggctccg cccgaaccgg
     2761 cgatgcgatg cgtctggatg tagcgatact gcgcgttgct cggggcgttc atcggatgac
     2821 cctcctgttg ggggaaatga tagccgtagg tggaaatgga tgggaagatg accccgatgt
     2881 gataccgggg tgagaatgct cgatggttct cggcgtctca gcgtcgccat gcatgataga
     2941 gggcgcgttg ctaacggcac cctgacgcgc ccgcctgagc gggaataagc tccatctccc
     3001 cgcggtatgg gtcgcggcta tcgcagcgag gacgccttgc cccaggacga cagacgccgc
     3061 cgcttcgagt tgctggccct gccgcatctc gacgcagcct acaatctggc ccgctggctg
     3121 gccggcaaca cgacggatgc cgaggacgtg gtgcaggacg cctatctccg tgcctaccgc
     3181 tattttgatg ccttccaagg ggggaatttt cgcgtgtggc tgctcaccat cgtccgaaat
     3241 gccttcatga cctgggtgaa ggagaaccgt tcgggcagga tggtgttcgt tcccgacacg
     3301 ccgctggcgg agtccgcgga cacagaagaa gctgcctggg gcagcaaacc gcgcgaccct
     3361 gaggcgctgc tgatggacag catcgacagc caaacgctta gccgtttgat ggagaagcta
     3421 ccgtcggact atcgcgaggt actgctgctc cgcgaggttg aagacctctc ctacaaggaa
     3481 atcgcagatg tcaccggcac gccgatgggc acagtgatgt cgcgcctgtc acgcgcccgg
     3541 ctggcgctcc gcaagttgtg gctggagcac gccgagttgg agaacgccga tggaatgtga
     3601 tcgcgcggcc ggcatgctgt cgccgctggt tgacagtgca ctgggaccat tcacgcgctg
     3661 gcgcgtgcgc cggcacgtgg ccggctgcac ggactgcgcc gacaagctgg aggaattgcg
     3721 cgccatgcag tcggcgatcc gcaccaagct gaccttccac cgcgcgcctc caggccttgc
     3781 cgctcgtatc ggcgccgccc tgccgcgcga ggccgcgccc gcaccgtccc ggccctggtt
     3841 ccgcatgccc gccttcagct tcggcagcac gggcctcgcc ggcgcattgg ccggtgtcgt
     3901 gttgaccctt ctggtggtgg gcggtcagcc gcagagcgat ctgctgcagg acgtcatcgg
     3961 cagccacatc cgttccttgc aggccgatca cctgaccgac gtgctgacct ccgaccagca
     4021 cacagtgaag ccatggctat cggcgcgcct cgacgtgtcc ccggccgtga aggaacttaa
     4081 ggaccagggc ttccccctcg tcggcggccg cgaggactac gttgacggcc accccgccgc
     4141 ggtcgttgtg tatagacacg acaagcatgt gatcaacctc ttcgcctggg cctcacccgg
     4201 ccgtcccgaa gaggcgttcc gcgatgagtc gcgccagggc ttcaacatcg tgacctggcg
     4261 ccgcggcggc atcacctact acgcggtgtc ggacgttgaa gccgatcagc tggcggattt
     4321 cgcgaagctg gtggcggaag ggacatagcg cccgccgcca ccagtcgtca tgacgttcca
     4381 gttgatcgtt cagccagcgc ccgcacccgc tggcggcgta taccacggca ctgcgatgcc
     4441 gaggcaagta accttcgggc cgtggatgtt ggcgatcagc catccatcct gccggatctg
     4501 accatcaacg gttgcagtgc gtccgtgacc gcggatttcc tgggcactca tcttgaaggt
     4561 gcgcttcacc atgtcatagc cgcccgtggc gtaggcgatg gacttcatat cgtcccatgc
     4621 gatcgtgccc gacagcgtat tgttcgcacg cacgacaata tcccagtcca gcgcaggaca
     4681 ggcaccttgg gccgtggaat ggaatacata cacacgccct tccggcaaac tttgggcggc
     4741 agcggcgccg aacgttgctg ccagcatcag cgcgccagcg gccaatgtgt tgatcatttt
     4801 catactttat gtatccttgc tttgtagaca cgacactgcc gcagcagacc cgactttgtt
     4861 gccgcagcag acggtcttgc ccggcgcggt tcaccatcat ccgttgtccg gcgatgtcat
     4921 tgatttggat caaggtcgat cgccgccatg ttgcgccact gtctgtccgt cgccttgctc
     4981 ctgttgttgc tcccaccgag ggcacacgca gacccggcag ccgatgcagc ccagtgcagg
     5041 caggctgccg gcagctatct cgtcggaaca gtgaccactg gcccgtcgtt ccgcggcggc
     5101 aagccgctgc acggcgtata tctgtcgcac acgcatctca cgctgctggg cgatgacggc
     5161 accagctacg acgttgcaat cgacaacgtc tttgccaacg gctatcgccg gaaccaagct
     5221 gcggtgccgc caccactcaa cagcatccgc accggtgaca ggctggaact atgcggcaag
     5281 cgttactcag gcggcgatgt cgggatcgac tgggtacaca ccaattgtgg cgaggctccc
     5341 tcgcacgaca agcccgacgg gtgggtccgc aaacttgatg ccaacggtac agccgacggc
     5401 aacatggaag ccagccaagc attttgcagc ctctggccga attgaggctc ccgccttgcg
     5461 ttgccgtccc aactgatgag cctagcgccg agcattgtcg ccaccgggca gcatctggtt
     5521 tgccgcgtcg atactcgctc ggttcgccgt cggtatgtag ggcagattgc gcaggacctg
     5581 cagcgtctgc tgcggcccat tcaggaaggc cggcggaaca aggaattgta gtgcggcctg
     5641 ctggttgcca gcctgccacg ccgctgcgaa ttgcagcaac gcgcccacga ccatctgcgc
     5701 cggcactccc agcggaacgc caagcacggc ccgcacatcg ccccgtgccc gcaacatttc
     5761 ctgcttggtc agcggcgaca ggtccaacca gcgagggctg ctgagctggc ccgccagata
     5821 gtcgattgcc gcgcaggccc gcgcgccatc aactgggtta ttcgccgtac gcgctggcga
     5881 ggcgaatgcc gatgaagcaa tattcgccgc gcccacatca ttatccatgg tgccgaaagc
     5941 cgcgaacggc agttgcgccg tatccggcgg gggagttgcc gccgcgcacc cagccaggga
     6001 gacgcacacc accatcacga cgatgcggga gagcatacaa aagcctccat gggatgccct
     6061 cgagcaagtg gggtgcgtaa acgcatcgcg ccaacccacc cgactatgtg gcgctgcgca
     6121 gcccagcacg gaactgcgcc gccaactccc ggtacacctc cgcattcaga tcgaacttgc
     6181 ggcgttcctc gtcgctcatc atgcggacca gccgcgcggg cgaaccaacc cacaactcgt
     6241 tccgcccaat cactttgcca ggtgtcagca gaccgccagc cccgagcatg ccgccttcct
     6301 cgatcaccgc cccatccagc acggtagccg cgatccccac gaatgcccgg tccttcagcg
     6361 tgcatgcgtg gatcaccgcg ttatggccca cggtgacatc gtcgccgatc gttgtggaga
     6421 acgcgcccga atcgacgtgg atgatcgcgc catcctgaat gttcgtccgc gccccgatgt
     6481 ggatgaagtt catgtcgccg cgcaccaggc agtggaacca gatgccgctt tccgaaccca
     6541 cctccacatc gccgatcacg gcagcggttg gagcaacaaa tgcgtcaggt gcgatctttg
     6601 gcgtgatgcc gttcagcgag tagatcggcc cgtcaggcat gcgccaaagc cggctgcgcg
     6661 ctcaccgaca gcccgagcat cagccgcata ttctgcaccg ccgccccgga cgcacccttg
     6721 ccgagattgt cgagccgcgc caccagcact gcctggcgct gcttttcgtt cgcatagacc
     6781 gacagttcca gcaggttggt atagttcagg ccctccggct ccagcttgcc atcggcggac
     6841 ggcgctacca cgcgcacgta atcacagccg cgatatcgcg cctccagtac cgtctgcaga
     6901 tcagccccac ttggcctgcc gttcagcgtg tcgagatgca gcggcacgga taccagcatt
     6961 ccctgccgat aatgcccgac cgacggcgcg aagatcggcc ggcgtgacag cccagagtag
     7021 agctgcgtct cgggcagatg cttgtgctca agcttcagcg catacagttc gaatgcggga
     7081 ccaccacgtt gttcgt
//
LOCUS       DATGEM010000127        12576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1447731, whole genome shotgun sequence.
ACCESSION   DATGEM010000127 DATGEM010000000
VERSION     DATGEM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12576
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447731"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VKQ27_03990"
     CDS             complement(<1..251)
                     /locus_tag="VKQ27_03990"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLY88121.1"
                     /translation="MSQSATNPPLIAVLNGPNMNMLGLRQPHLYGSATLDDVEQLCAE
                     AADELGLAIDFRQTNGEGELISWIQECRGRAQGIVINPAG"
     gene            421..1797
                     /locus_tag="VKQ27_03995"
     CDS             421..1797
                     /locus_tag="VKQ27_03995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88122.1"
                     /translation="MIMTKPDKPRRQLRFGAFMNVPSSHPTGWRHPDAIPETDLSFRH
                     LADMARMAEHGRLDAMFFQDSVAIPGSAGLYGGKPFNPRSGRQAHIEPVSAIAGLAAI
                     TSHLGLISTCTTSYNEPYNVARRFLTIDHISGGRVGWNLVTSQAEDEAMNFGLDAHMD
                     HALRYERAAEFHDVVAALWDSWEDDAFLRDKASGVWFDHTKMHFLNHKGKHFTVRGPL
                     NVARSPQGRPVIAQAGSSDVGMELAARTADMVFTAQPTIRDGQAFCAEMRTRAAKYGR
                     GPDHLRIFPGLMPIVGRTENEAREKYEELRELVDDEVGLRGVARLCGGLNIYDYPPDG
                     PLPPLPPSNAARARQEMIIAMGKRGMTVREIGRVLGMSQAHRVAFGTPRQLADDMQEW
                     LEADACDGFNLLFAHYPKPLEEFCQLVVPELQRRGIFRTEYEGTTLRANLGVPVPPNV
                     FAQEPEHA"
     gene            1790..3154
                     /locus_tag="VKQ27_04000"
     CDS             1790..3154
                     /locus_tag="VKQ27_04000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88123.1"
                     /translation="MRRRQLHLGGFFSTPGNHLAGWRHPHAVPTADMDFRLYAHITQV
                     VERAKFDTIFFQDTAAVNGSRAMARGDMTRTRISRIVKLEPTTLLCALAAVTSHIGLI
                     ATATTTYNEPYNIARRFMSIDHISEGRAGWNLVTSQIEDEAQNFGYAEHVDHAKRYAR
                     AAEFYDVVVGLWDSWEDAALLRDKARGIYMDRDKVHFLDHEGEHFKVRGPLNITRSAQ
                     GWPVVAQAGSSEAGRELAAKTADVVFTAQTQIDEARVFYADVKARAARYGRSAEDIVI
                     MPGITPVLGATMAEARERYEELQALLPDDLALQSMSHISGGLDLSKFPLDGPLPDLPP
                     SNAAKARQALVVKTAREQNMTLRQIARHTAAAMGHRVLVGTAEYIADELEGWLKADAA
                     DGFNVICNHYPKPLEDFCQMVVPELQRRGVFRTEYEGTTLREHLGLRVPENRYTLRQR
                     QAAD"
     gene            complement(3158..4123)
                     /locus_tag="VKQ27_04005"
     CDS             complement(3158..4123)
                     /locus_tag="VKQ27_04005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY88124.1"
                     /translation="MEHVRLGRAGLRVSRLGLGAMGFGDRSWRSWVLDLEQSRAVFRR
                     AMAAGINFIDTCDYYSAGASEEIVGTLVAESGNRVELVIATKVGNPMGRDANARGYSR
                     KHIIAAAEASLRRLRTDYIDIYQTHIWDPTTNLEEMVEAFDHLVQSGKVLYVGITDMP
                     FWQFASAYFFAERHGLARFASVQNHYNLLWRDDERELLPFCRARGIGLIPYSPMARGF
                     LCGRARRQGPTWSERAKTDDYTYKLYGRPADEAIVDRVAELAASRGVSAAQIALAWVL
                     HQPGVTAPIIGATQVSHVDEAVAALEITFSSAELQQLDELYVPRR"
     gene            complement(4139..5671)
                     /locus_tag="VKQ27_04010"
     CDS             complement(4139..5671)
                     /locus_tag="VKQ27_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88125.1"
                     /translation="MTMLRIGRRQTLAGLAGGMLVSRAGLAQTPVHGGTLTVGLSNEA
                     KTYDPIFSVQFTERYVLYLAFDTLVKYGPDFSIKPELAESWDTSADGKRIVFTLRQGV
                     QFQDGTPFDAAAVKWNIDQRLDPKVNSPQRQVLAPIVASVDVIDPQHVAFNLVAPSPA
                     LFSLLGERVGFMVSPTAWQQRGDAFGSQPIGTGAFVLKEWTRGSRVVLERNPHYWQAG
                     LPYLDRIVVQDLAGSVIGVQRLLTGEIDYVDQLTPADVLPIEKRAGIVLKPITVGRWY
                     FLQWHVNKAPFDNPKLRQAFAHAIDRNRLNEITMRGQGTISDSPTPKGLWWYDPDLKS
                     YPHDPALAKTLLAEAGFTNGFEYTLSTPQVTVFQQVNQLLQEQLGAIGIKLQLQPVAA
                     SEWYARVVSGTTNLTPTRWTQRADPDGLLYILFHSRGFANTMKYRNEKVDALLDQART
                     VYDVPTRKKLYAAAQQQIVDDLPMVPLIFGAEYAALRAPIQGFEWIPDEIPRFRDLWK
                     SA"
     gene            complement(5682..6458)
                     /locus_tag="VKQ27_04015"
     CDS             complement(5682..6458)
                     /locus_tag="VKQ27_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88126.1"
                     /translation="MRRPGLCFGAGVVACIAVIAAAAPLVAPRDPYASGIDMLVPSSA
                     LHLLGTDDLGRDVLSRVIYGARASLAIGFGAALAAMVIGVPIGLVAGALRGRVDTVLA
                     ALIDLFIALPGLVLALIITVMVGANLRNLVLVLGFVMWPPIARLVRGQAMALREMLFV
                     EAARATGGTPLWIIRRHIWPGVLPIVAAQFATSVAFAIFTSASLSFLGLGIPPPTPDW
                     GGMVRAGYDYLAINPWMSLAPGAAVSLTVMGFYVMGRSFR"
     gene            complement(6455..7396)
                     /locus_tag="VKQ27_04020"
     CDS             complement(6455..7396)
                     /locus_tag="VKQ27_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88127.1"
                     /translation="MLGLIVRRVLLMLATAIAISALLFFAVTQLLGSPAAMMLGQDAS
                     PQAIAALNLKYGFDRPAIVQYLHWLTGALTGDLGRSYTTQQSVAAAIATRIPVTLELS
                     LWSIALAVIAAVVSNTIPTGRRVLQPVVTAANLIGITVPNFMLGVSLIFILSVKLDLL
                     PSTGWVPWSDGVGAHLEHMIMPVLTLSAYYFGAFSIVYRAEYQNVLRRPFVRVAQAKG
                     LGEWRVAFRHAAPNAILPVITFVGISMGQLAGGAVVTETIFSMPGTGRLFVASIESRD
                     FPVMLAIGMLIVVGVVIMNLLADIAYAVVNPQIRHDL"
     gene            7522..8700
                     /locus_tag="VKQ27_04025"
     CDS             7522..8700
                     /locus_tag="VKQ27_04025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88128.1"
                     /translation="MQFGLFHNLHDLTKQRDYADLLDELRDLAAICEETDFDVLWMPE
                     HHFSVWGRELLPNPLMTAVDIAARTKRIRIGLAAAIVTFWHPIRAAEDIALLDQLTRG
                     RVEIGFGRGNYGLEASNLNPEANPNDQAKSKQVFDEAVSVIKAALTQERFSFKGQVYQ
                     FPAPGFRADRAHSVTDRDYVDPATGELIKLSIYPRARQNPLPMWEVVNSVDSIQHAAR
                     QDLGIIMWRPPVAALRERLRVYRDTLRQATGQDLPLGARGAIMRDTFVADSEREAERI
                     AGGPMMESLNFSNWRGPSVYLNPGETLPAEQEAALKQRLTYDFVRDRSVFFGPPERIV
                     QQILQLHRETGISSVVFKTSWSGLPHAAARESVRRLGTEVIPAIRAALRAERQPVAAE
                     "
     gene            complement(8704..9417)
                     /locus_tag="VKQ27_04030"
     CDS             complement(8704..9417)
                     /locus_tag="VKQ27_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLY88129.1"
                     /translation="MVGASFLSSIRSAITPPLVGPRQHTRAERSAQSLREAIVSLRLA
                     PGTRLVERELVDQLGVSRTGVRAALQSLQAEGLVVRGRRGVFAVASISCDEARQIYEV
                     RAALEPAMARLFVACATKEQITALGDAVARAEAAADARDGPAYVEAFRSFYAVLLAGS
                     GNEIAQRILETLEARISYLRHLTTQLAPQTRQRRTAALLRSIFNAVRRRAPELAAQRC
                     AAFVERSAQFALDVLNDHA"
     gene            9389..10171
                     /locus_tag="VKQ27_04035"
     CDS             9389..10171
                     /locus_tag="VKQ27_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY88130.1"
                     /translation="MLERNDAPTIRYWDAGGDRPPVMLIHGVGADGTSWDQIAAAMSS
                     QFRVLRLDLRGHGRSGHIQGALTIDDFVRDVVDVLDACGVGAAHIVGFSLGGMIAQGL
                     ALQHADRVQRLVLLSAVAGRTVEERERVQARLTILREQGVSAITGAAQERWFTPEFIA
                     RNPDLVEQRMRQLRENHAPSYAAAYTVFSTTDLADRLHAIQAPTLVATGEGDVGSNTR
                     MARYMHEQIRGSRLAILPGLRHSVLVEAPGLVTQLVSEFLRE"
     gene            10253..10465
                     /gene="thiS"
                     /locus_tag="VKQ27_04040"
     CDS             10253..10465
                     /gene="thiS"
                     /locus_tag="VKQ27_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HLY88131.1"
                     /translation="MQTAVTLTLNGEARAFDGPMTVASLLASLGLEMRKVAVERNEEI
                     VPRSVYAETWLQSGDALEIVHFIGGG"
     gene            10470..11261
                     /locus_tag="VKQ27_04045"
     CDS             10470..11261
                     /locus_tag="VKQ27_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HLY88132.1"
                     /translation="MDGSLPQDDTWSVAGLQFRSRLIVGTGRYKDLEETGAAIAASGA
                     EIVTVAVRRVNLSDPKAPMLQDYVDPKKYTYLPNTAGCHTAQEAVRTLRLAREAGGWN
                     LVKLEVLGPPPTLYPDMRGTLEAAEALIKDGFEVMVYTADDPVACKRLEEMGCVAIMP
                     LGAPIGSGLGIQNAAMIWMIREQTKVPFLVDAGVGTASDAAIAMELGCDAVLLNSAIA
                     HAKDPIRMAKAMKLAIEAGRQAFLAGRMPRRLGADPSSPLTGLIR"
     gene            complement(11266..12018)
                     /locus_tag="VKQ27_04050"
     CDS             complement(11266..12018)
                     /locus_tag="VKQ27_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88133.1"
                     /translation="MHWCLGMYASGSTWLFNVAMHIAATLAPEKPPIGRYITDISELA
                     PDQGAIDRLFVKTHYVDNASAEALCRLAASLLISIRDPRDCISSLMLYQHYPLELALQ
                     AVEASALCCERFAIGPRTLLLRYENGFTGEPGTLDRIAVMLSGHLTDEDRARIFAANS
                     RISVEARIAALDTLPTAIHDARTGDIVDPTTQWHRHHANRSGEVGRWRHLLTLADVAI
                     IEQRLGDWMHRSGYAPEVAPYTRADSSQVLRL"
     gene            complement(12103..>12576)
                     /locus_tag="VKQ27_04055"
     CDS             complement(12103..>12576)
                     /locus_tag="VKQ27_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88134.1"
                     /translation="WQQLADRAEMIGRPVPMHATTAEPGLHLLAKGRAVKPIYSDKAL
                     AIFALPRGATEVRLVSRAQSPTMACPWLEDRRRLGVRVARIVLRGADDLCEVPVDHPD
                     LVKGWWAVEREGIGLRRWTNGEAVLPLPTLDGDVMLEIHLGGEMTYLADDELRAA"
BASE COUNT         2328 a         3915 c         4198 g         2135 t
ORIGIN      
        1 ccggcggggt tgatcacgat cccctgcgca cggccacggc attcctggat ccaggagatg
       61 agctcgccct cgccattggt ctggcggaag tcgatcgcaa gcccgagctc gtcggcagcc
      121 tccgcgcaga gctgctccac atcgtccagc gtggcgctgc cgtagagatg cggctggcgc
      181 aggccgagca tgttcatgtt cggtccgttc aggacagcga tgaggggcgg attcgtcgca
      241 gattgggaca tacccgcgcg tagcataggt gggagcgggg gtccaacgct ggcagactca
      301 gcgccaatgg acattccaga ccattgcgtc cagcctggag acgacgccag ccaagaagcg
      361 ttgaacgttc gagagcaggc ctaattcgtt gaccaacctg ccgccgaaag ccagccgccg
      421 atgatcatga cgaaaccgga caagcccagg cgccaacttc ggttcggcgc gttcatgaac
      481 gtgcccagca gccatccgac cggatggcgc cacccggacg cgatccccga gaccgacctc
      541 agtttccgcc atcttgccga catggcgcgc atggctgaac acggcaggct ggatgcgatg
      601 ttcttccagg acagcgtggc gatccccggc agtgccgggc tgtatggcgg caagccgttc
      661 aatccgcgca gcgggcggca ggcacatatc gagccggtca gcgcgatcgc cgggctcgcg
      721 gcgatcacgt cgcatctcgg gctgatctcg acatgcacga ccagctacaa cgaaccatac
      781 aatgtcgcgc ggcggttcct gaccatcgac cacatcagcg gcggccgcgt cggctggaac
      841 ctggtgacgt ctcaagccga ggacgaggcg atgaacttcg gcctcgatgc gcatatggac
      901 catgcgctgc gctatgaacg cgcggccgag tttcacgatg tcgtggcagc attgtgggac
      961 agctgggagg acgatgcgtt cctccgcgac aaggcaagcg gcgtctggtt cgaccacacg
     1021 aagatgcatt tcctcaatca caaggggaag catttcacgg tgcgcggtcc gttgaatgtc
     1081 gcacggtcgc cgcagggacg gccggtgatc gcgcaggcgg gctcatcgga cgtggggatg
     1141 gaactggcgg cgcgaaccgc ggacatggtg ttcaccgcgc agccgaccat cagggacggg
     1201 caggcgttct gcgcggagat gcgcacacgc gcggcgaagt atggccgggg gccggatcat
     1261 ctgcggattt ttccagggct gatgccgatc gtcggccgga cagaaaacga ggcgcgggag
     1321 aaatacgagg aactgcgcga actggtggac gacgaagttg ggttgcgcgg cgtggcccgt
     1381 ctgtgtgggg gactgaatat ctacgactac ccgccggatg ggccactgcc gccgctgccg
     1441 ccaagcaacg ctgcgcgtgc gcggcaggag atgatcatcg cgatgggcaa gcgcggcatg
     1501 actgtacgcg agatcgggcg tgtgctgggg atgtcccagg cgcatcgcgt ggcattcggc
     1561 acgccgcggc agctcgccga cgatatgcag gagtggctgg aggcggatgc gtgcgacggg
     1621 ttcaacctgc tgttcgcaca ttatccgaag ccgctggagg aattttgcca gttggtggtg
     1681 ccggaattgc agcggcgcgg gattttccgc acggagtacg aggggacgac gctgcgggcc
     1741 aatctcggcg tgccggtgcc gcccaatgtg ttcgcacagg agccggagca tgcgtaggcg
     1801 gcaactgcat ctgggcggat tcttcagcac acccggcaat catctggcgg gctggcggca
     1861 tccgcacgcg gtgccgacgg cggatatgga ctttcgtcta tacgcacata tcacgcaggt
     1921 ggtggagcgg gcgaagttcg acacgatctt cttccaggac acggcggcgg tgaacggcag
     1981 ccgggcgatg gcgcgcggtg acatgacccg gacgcggatc agcaggatcg tgaagctgga
     2041 gccgaccacg ctgctgtgcg ctttggcggc ggtgacctcg catattgggc tgatcgcgac
     2101 agcgaccacg acatacaacg aaccatacaa catcgcgcgg cggttcatgt cgatcgacca
     2161 catcagcgaa ggccgcgcgg ggtggaacct ggtaacctcg cagatcgagg atgaggcgca
     2221 gaatttcggc tatgccgagc atgtcgatca tgcgaaacgc tacgcccgcg ccgcggaatt
     2281 ctatgacgta gtggtggggc tgtgggacag ttgggaagat gcagcgctgc tgcgcgataa
     2341 agcgcgcggt atctacatgg accgggacaa ggtgcatttc ctcgaccatg agggagagca
     2401 cttcaaggtg cgtggaccgc tgaatatcac gcgctcggcg caagggtggc ctgtagtggc
     2461 acaggcgggc tcgtcggagg cagggcggga actggcggcg aaaacggcgg atgttgtgtt
     2521 cacggcgcag acgcagatcg atgaggctcg ggtgttctat gcggatgtga aagcacgcgc
     2581 tgcacgctac gggcggtcag cggaggatat cgtgatcatg cccgggatca cgccggtgct
     2641 tggcgcgacg atggcggagg cacgcgagcg ctacgaggaa ttgcaggcgc tgcttcccga
     2701 tgacctggcg ctgcagtcga tgtcgcacat cagcggcggg ctcgatctgt ccaagttccc
     2761 gctggatggg ccgctgccgg atctgccgcc gagcaatgcg gcgaaggcgc gacaggcgct
     2821 ggtggtgaag acggcacgcg agcagaacat gacgctgcgg cagattgccc ggcacaccgc
     2881 ggctgcgatg gggcaccgcg tgctggtcgg caccgccgag tatatcgcgg atgagctgga
     2941 gggttggctg aaggcggacg cggcggacgg gttcaacgtg atctgcaacc attatccaaa
     3001 gccgctggag gatttctgcc agatggtggt gccggaattg cagcggcgag gggtattccg
     3061 gacggaatat gaggggacga ccctgcgcga gcatcttggg ctgcgggtgc cggagaaccg
     3121 ctacacgctg cggcagcggc aggcggcgga ttgagcgcta tctgcggggt acgtacagct
     3181 cgtcgagttg ctgcaactcg gctgagctga acgtgatctc gagtgcggcc acagcttcgt
     3241 cgacgtggct cacctgggtc gctccgatga tcggggcggt gacgccaggt tggtggagca
     3301 cccaggccag ggcaatttgt gcggcactca cgccccttga ggcagcaagc tccgcaacgc
     3361 ggtccacgat cgcctcgtca gcgggtcgcc catacagctt gtaggtatag tcgtcggtct
     3421 ttgcccgttc cgaccacgta ggaccctgcc ggcgcgcgcg tccgcacagg aagccgcgag
     3481 ccatcggact gtaggggatc aatccaatcc cccgtgcccg acagaacggc agcaactcgc
     3541 gttcatcgtc ccgccatagc aggttgtagt gattctgcac cgaggcaaat cgtgccaggc
     3601 cgtgccgttc ggcgaagaaa tacgcactgg cgaactgcca gaacggcatg tcggtgatgc
     3661 cgacgtacag caccttgccc gattgcacga ggtgatcgaa tgcctcgacc atctcctcca
     3721 gattggtcgt cgggtcccag atgtgcgtct gatagatgtc gatgtagtca gtccgcaggc
     3781 gacgcagtga cgcttccgct gccgcgatga tgtgcttgcg cgagtagccg cgcgcattgg
     3841 cgtcgcgccc catcggattg cccaccttgg tcgctatcac cagttccacg cggttgccgg
     3901 attcggccac gagcgtgccg acgatctctt cgctcgcgcc cgcggagtag taatcgcagg
     3961 tgtcgatgaa gttgatgccg gcggccattg cgcgacggaa caccgcgcgg ctttgttcca
     4021 ggtccagcac ccaggatcgc catgaccggt cgccgaatcc catagcgccg aggccgagcc
     4081 gcgaaacccg cagtcccgcc cgcccaagtc tgacatgttc catacggatg cctcctgctc
     4141 aggctgattt ccagagatcg cggaagcgcg gaatctcgtc gggaatccat tcgaagccct
     4201 ggatcggtgc gcgcaaggct gcgtattccg caccgaagat cagcggcacc atcggcaggt
     4261 catcgacgat ctgctgctgg gctgcggcat agagcttctt tcgcgtcggc acgtcgtaca
     4321 cggtccgggc ctggtcgagc agcgcgtcga ccttctcgtt cctgtatttc atcgtgttgg
     4381 cgaagcccct gctgtgaaaa aggatgtaca acaggccatc cgggtcggcc cgctgtgtcc
     4441 accgggttgg cgtaagattg gtggttcctg agaccacgcg cgcataccac tcactggcag
     4501 cgacaggttg caattgcagc ttgatcccga tggcaccgag ttgttcctga agcaactgat
     4561 tgacctgttg gaacaccgtc acctgcggcg tggacagcgt atattcgaag ccgttggtga
     4621 accccgcttc cgccaggagt gtctttgcta gtgcggggtc atgtggatag gacttcaggt
     4681 caggatcgta ccaccacaac cctttcggcg tcggcgaatc cgatatggtg ccctggccgc
     4741 gcatggtgat ctcgttcaac cggttccggt cgatcgcatg ggcaaacgcc tgccgcagct
     4801 tcgggttgtc gaatggcgct ttgttgacgt gccattgcag aaagtaccaa cgcccgaccg
     4861 tgattggctt cagcacgatg cccgcgcgct tctcgatcgg aagcacgtcg gccggcgtca
     4921 gctggtcgac gtaatcgatc tcacccgtca ggagtcgctg cacgccgatg actgatcccg
     4981 ccagatcctg cacgacgatg cgatcgagat atggcaggcc ggcctgccaa tagtgcggat
     5041 tacgctccag caccacgcgg cttccgcgcg tccattcctt caacacgaag gcacccgtgc
     5101 cgatcggctg actgccgaag gcatcgccgc gctgctgcca ggcggtcggc gataccatga
     5161 accccacccg ttcgccgagc aggctgaaca atgccggcga gggcgcaacc agattgaacg
     5221 cgacatgctg cgggtcgatg acatccaccg aggccacgat cggcgccagc acctgccgtt
     5281 gcggcgagtt gacctttggg tccagtcgct gatcgatgtt ccacttcacc gcggcggcgt
     5341 cgaatggcgt gccgtcctgg aactgcactc cctgtcgcaa cgtgaacacg atccgcttgc
     5401 cgtcggcgga ggtgtcccag ctttctgcca gttcgggctt gatggagaag tcgggcccgt
     5461 acttcaccag cgtgtcgaag gccaggtaca ggacgtagcg ttcggtgaac tgcaccgaga
     5521 agatcgggtc gtaggtcttc gcctcgttcg acaggccaac ggtcagcgtg ccgccgtgta
     5581 caggggtttg cgccaggccg gcgcggctta ccagcatccc tccggccagt cccgccagcg
     5641 tctggcgccg tccaatgcgc agcatcgtca tcacgctccc ctcacctgaa tgatcgaccc
     5701 atcacgtaga aacccatcac cgtcaggctg acggccgcgc ccggtgccag gctcatccac
     5761 ggattgatcg ccagatagtc gtatccggca cgcaccatgc cgccccagtc cggtgtcggc
     5821 ggtggaatgc ccaatccaag gaaactgagg cttgccgagg tgaagatcgc gaacgcgacg
     5881 ctggtcgcga attgcgccgc cacgatcggc agcacgccgg gccagatatg gcggcggatg
     5941 atccaaagcg gcgttccgcc ggttgcccgc gccgcctcga cgaacagcat ttcgcgaagt
     6001 gccattgcct ggccgcgcac cagccgggct atcggtggcc acatcacgaa gccaagcacc
     6061 agcaccagat tgcggaggtt cgcgccgacc atgacggtga tgatgagcgc aagcacaagg
     6121 ccgggcagcg cgatgaacag gtcgatcagc gcggcgagga cagtgtccac ccttccccgc
     6181 agtgcgcctg cgacgagccc gatcggcaca ccgatgacca tggccgccag tgccgcgccg
     6241 aagccaatcg ccagcgaggc tcgtgcgcca tagatgacac ggctcaacac gtcgcgcccc
     6301 aggtcatcgg tgcccagcaa atgcagcgcg ctcgacggca ccagcatgtc gatccccgag
     6361 gcatacggat cgcgcggcgc caccagcgga gcggcggcag cgatcacggc aatgcaggcg
     6421 acgacaccgg cgccgaagca aagtccggga cgtctcacaa gtcgtgccgg atctgtggat
     6481 tgaccactgc gtaggcgatg tcggccagca ggttcatgat caccacgccg acgacgataa
     6541 gcatgccgat cgcgagcatg acggggaagt cgcgtgattc gatggaagcg acgaacagcc
     6601 ggccggtgcc cggcatggaa aagatggtct cggtcaccac cgcaccgccg gcaagctggc
     6661 ccatcgagat gcccacgaac gtgatcacgg gcaggatcgc gttgggcgcc gcatgacgaa
     6721 acgcgacacg ccattcgcca aggcctttgg cctgcgccac gcgcacgaac ggacgccgca
     6781 atacattctg atattcggca cggtacacga tgctgaatgc gccgaaataa taggccgaca
     6841 gagtcagcac cggcatgatc atgtgttcca gatgcgcgcc aacgccgtcc gaccaaggta
     6901 cccagccggt tgacggcagc agatccagct ttaccgacag gatgaaaata agcgacacgc
     6961 cgagcatgaa gttcggcacc gttatgccga tcaggtttgc tgcggtgacc acgggttgca
     7021 gcacccggcg cccggtcgga atggtgttgc tgaccactgc cgcgatcacc gccagtgcga
     7081 tcgaccacag ggagagttcc agcgtcaccg gaatacgcgt ggcgatcgcg gctgccacgc
     7141 tctgttgcgt cgtgtagctg cggccgagat cgccggtcag cgcgccggtc agccaatgca
     7201 gatactgcac gattgccggg cggtcgaagc cgtatttgag attgagggcc gcgatcgcct
     7261 gcgggctggc atcctggccc agcatcatcg ccgccggact gcccagcagt tgcgtcaccg
     7321 cgaagaacaa cagggcggat atggcgatcg cagtcgccag catcagcaga acgcggcgca
     7381 caatcagccc gagcatcagc ctgaaccatc cccttcccgc cgttccaccc gcggagccgg
     7441 cgcttggctt cgccagctct gccatggtat accaaactgc tttggtatac caaaggggct
     7501 ggcgtccaag ggaggcacgg catgcaattc ggactgttcc acaacctgca cgacctgacg
     7561 aagcagcgcg attatgccga tctgctcgac gaactgcggg atctcgcagc aatctgcgag
     7621 gagaccgatt tcgatgtgct gtggatgccg gagcaccatt tcagcgtctg gggccgcgag
     7681 ctgctgccca atccgctgat gacggcggtc gatatcgccg cgcgcaccaa gcgcatccgc
     7741 atcggcctcg ccgcggcaat cgtgacgttc tggcatccga tccgcgccgc cgaggacatc
     7801 gccctgctcg accagctcac ccgcggccgc gtcgagatcg gcttcggccg cggcaactat
     7861 ggtctggaag ccagcaacct caatcctgag gcaaatccga acgaccaggc gaagagcaag
     7921 caggtgttcg atgaagcggt atcagtgatc aaggccgcgc tgacgcagga gcgattttcc
     7981 ttcaagggtc aggtgtatca attcccggcg ccaggtttcc gcgctgaccg cgcccactct
     8041 gtgaccgacc gcgattatgt cgatccggcg acaggcgagt tgatcaagct gtcgatttat
     8101 ccgcgtgccc gccagaatcc gctgccgatg tgggaggtgg tgaattccgt cgactccatc
     8161 cagcatgcag cacgccagga cctgggcatc atcatgtggc gcccccccgt cgcggcattg
     8221 cgggaacgcc tacgcgtcta ccgtgacacg ttgcgccagg ccaccggaca ggacctgccg
     8281 ctcggcgccc gcggcgcgat catgcgtgat acgttcgttg cagatagcga acgcgaagcc
     8341 gagcgcattg ccggcggacc aatgatggaa tcgctcaact tctccaactg gcgtggaccg
     8401 agcgtctatc tgaacccggg ggagacgctg ccagccgagc aggaagcagc gctcaagcaa
     8461 cggctcacct acgacttcgt gcgcgatcgc agcgtgttct tcgggccgcc ggagcgcatc
     8521 gtgcagcaga tcctgcagct gcatcgcgag accggcatct ccagcgtggt cttcaagact
     8581 tcgtggtccg ggctgccaca tgccgcggcg cgtgaaagcg tccgccgcct cgggaccgag
     8641 gtgatccccg cgattcgcgc cgcgttgcgg gccgagcggc agccagtcgc cgcggaatga
     8701 tgttcaggcg tggtcattca gcacatcgag cgcgaactgc gccgatcgtt cgacaaatgc
     8761 cgcgcagcgt tgggcagcga gttcgggcgc gcgtctccgc acggcgttga agatgctgcg
     8821 cagcagggcc gcggtacgcc gttgccgcgt ctgcggtgca agctgcgtcg tcagatgccg
     8881 cagatacgat atgcgagcct cgagcgtctc caggatacgc tgcgcgattt cgttgccgct
     8941 gccagccagc agcacggcgt agaaactgcg gaacgcctcg acataggcgg gaccatcacg
     9001 tgcatccgcc gctgcctcgg cgcgcgcgac cgcgtccccc agcgcggtga tctgttcctt
     9061 ggtcgcgcag gcgacgaaca gccgcgccat cgccggctcc agcgcggcac gcacctcgta
     9121 gatctgccgc gcctcatcgc acgatatgct ggcaaccgcg aatacgccgc gccggccgcg
     9181 caccacgagg ccctcggcct gaagcgattg caacgccgcg cgaacgccgg tccggctgac
     9241 gccgagctgg tccaccaact cgcgttccac cagccgtgtt ccgggcgcga gacgaagcga
     9301 tacgatcgcc tcgcgcaggc tttgtgcgct gcgttcggcc ctggtgtgct gtctgggtcc
     9361 cacaagggga ggagttatgg cagagcgaat gcttgagaga aacgacgctc ccaccatccg
     9421 ctactgggat gctggcggtg acaggccgcc cgtgatgctg atccatggcg ttggcgcgga
     9481 tggcacgagc tgggaccaga tcgccgcggc catgtcgtca cagttccgcg tgttgcgtct
     9541 tgatctgcgt ggccatggac gatccggcca tatccagggc gcgttgacga tcgacgattt
     9601 tgttcgcgac gtggtggacg tgctggatgc ctgtggtgtt ggggccgcgc atatcgtggg
     9661 cttctcgctg ggcggcatga tcgcgcaggg gctcgcacta cagcacgcgg accgggtcca
     9721 gcggctggtg ctgctgagtg ccgtggcggg gcgcacggtg gaggagcgcg agcgggtgca
     9781 ggcccggctg acgattctca gggaacaggg ggtctccgcc atcaccgggg ccgcacagga
     9841 acggtggttc acgcccgaat tcatcgcccg caatcccgat ctggtggaac agcggatgcg
     9901 gcaattgcgc gagaaccacg ccccgtccta tgccgccgcc tacacagtgt tttcgaccac
     9961 cgacctggcg gaccggctgc atgcgatcca ggcgccgacg ctggtcgcaa cgggtgaggg
    10021 cgatgtcggg tccaacacgc ggatggcgcg ctacatgcac gagcagatca ggggttcgcg
    10081 gctggcaatt ctgccggggt tgcgccacag cgtcctggtc gaggcgccgg ggctggtgac
    10141 gcagttggtg agcgaattcc tgcgggaatg aagcgccggc ccggggcgct ggcaggacga
    10201 tgtcacagca ggcttccttg ccccaccgcc gccgggctcc cataatccag ccatgcagac
    10261 tgccgttact ctcaccctga atggcgaggc gcgggcgttc gacggaccga tgaccgttgc
    10321 ctcgctgctc gcctcgctcg ggctggaaat gcgcaaggtg gcggtggagc ggaacgagga
    10381 gatcgtgccg cgctcggtgt acgccgagac ctggctgcaa tccggcgacg cgctggagat
    10441 cgtgcatttc atcgggggag gctgacgcga tggacggatc tttgccccag gatgacacgt
    10501 ggtcggtggc cggcctgcag ttccgctcgc gcctgatcgt agggaccggg cgctacaagg
    10561 acctggagga gaccggtgcc gcgatcgccg cctcgggcgc ggagatcgtc accgtggcgg
    10621 tgcggcgggt gaacctgtcg gaccccaagg cgccgatgct gcaggactac gtcgatccga
    10681 agaaatacac ctacctgccg aacaccgcgg gctgccatac cgcgcaggaa gcggtgcgca
    10741 cgctgcgcct ggcgcgcgag gcgggtggct ggaacctggt gaagctggaa gtgctgggac
    10801 cgccgccgac gctgtatccg gatatgcgcg gcacgctcga ggcggccgag gcgctgatca
    10861 aggatggctt cgaggtgatg gtctacaccg ccgacgaccc ggtggcgtgc aagcggctgg
    10921 aggagatggg ctgcgtcgcg atcatgccgc tgggggcgcc gatcgggtcg ggcctgggaa
    10981 tccagaacgc ggcgatgatc tggatgatcc gcgagcagac caaggttccg ttcctggtgg
    11041 atgccggcgt cggtaccgcc tcagacgcgg cgatcgcgat ggaattggga tgcgacgcgg
    11101 tgctgctgaa ttcggcgatc gcgcacgcca aggatcccat ccgcatggcg aaggccatga
    11161 agctggcgat cgaggcgggg cggcaggcat tcctggccgg gcggatgccg cggcggctgg
    11221 gggctgatcc atcgagcccg ctgaccggat tgatccggta ggaaactaca ggcggagcac
    11281 ctgagaagaa tccgctcggg tgtaaggggc gacctcaggc gcataaccgg accggtgcat
    11341 ccagtcaccg agacgctgct cgatgatcgc cacatcggcc aaggtcagca aatgccgcca
    11401 gcggccgact tcgccgctgc ggttcgcatg gtggcggtgc cattgcgtgg tcggatcgac
    11461 aatgtcgccg gtcctggcgt cgtggatcgc ggttggaagt gtgtccagcg cggcaattct
    11521 ggcttcgaca ctgatgcgac tgttggccgc gaaaatgcgg gcacgatctt cgtccgtcag
    11581 atggccgctg agcatcacgg caatcctgtc gagtgtgcct ggttcgccgg tgaagccatt
    11641 ctcataacgc agcagcagcg tgcgcggacc gatggcgaac cgctcgcagc acagggcaga
    11701 cgcttcaacg gcctgcaatg caagctccaa cggataatgc tgataaagca tgagcgatga
    11761 gatgcagtcg cgcggatccc ggatcgagat cagcagcgag gctgccaggc gacagagagc
    11821 ttcggcggat gcgttatcaa cgtaatgggt cttgacgaag aggcgatcga tcgcgccctg
    11881 atccggggcc agttcactga tgtcggtgat gtagcgcccg atgggaggct tttccggcgc
    11941 aagcgtggcg gcgatgtgca ttgcgacatt gaacagccag gtcgagccgc tggcatacat
    12001 accaaggcac caatgcatgt cgcggcatcc cgtgcgagtc acccgtggtc gagaaacagg
    12061 ggcggctgag gctgcccctg tttcttatcg tcagccggac cgtcaggcgg cgcgaagttc
    12121 atcgtcggcg agataggtca tttcgccgcc caggtggatc tcgagcatca cgtcgccatc
    12181 cagggtggga agcggcagca ctgcctcacc gttggtccag cgacggagac cgatgccctc
    12241 gcgttcgacc gcccaccagc cctttacgag atccggatgg tcgaccggaa cctcgcacag
    12301 atcatcggca ccgcgcagca cgatgcgcgc cacccgaacg ccgagccggc gccgatcctc
    12361 gagccacggg caggccatgg tgggtgactg tgcccgcgag accagccgta cctcggttgc
    12421 gccgcgcggc agtgcaaaga tcgcaagtgc cttgtcgctg tagatcggct tgaccgcccg
    12481 ccccttggcg agcaggtgca ggcccggttc ggccgttgtt gcatgcatcg gcacagggcg
    12541 gccgatcatc tcggcccggt ctgccagctg ctgcca
//
LOCUS       DATGEM010000128         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_647027, whole genome shotgun sequence.
ACCESSION   DATGEM010000128 DATGEM010000000
VERSION     DATGEM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647027"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VKQ27_04060"
     CDS             complement(<1..511)
                     /locus_tag="VKQ27_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLY88135.1"
                     /translation="MIIEMRTYTVRPGTIAEVEKRFGEHLPNREKHGKQLVAVWHSEV
                     GQLNQFIHVWAYDNFEQRTTVRGAAMKAGGWPPPIGEFLVDMKNEIFYPAPFSPPLEA
                     RQIGPLFEIRQYTLAPGSIPGLIERWSEKIEGRQKFSPLVAGMYTEFGALNKWVHIWA
                     YKDANERNSV"
     gene            complement(542..985)
                     /locus_tag="VKQ27_04065"
     CDS             complement(542..985)
                     /locus_tag="VKQ27_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLY88136.1"
                     /translation="MNVWDWVKAVHVLCAVIWVGGMFFAYVVLRPSLSVLEPIQRIAL
                     HTQVFRRFFLVIWHVVPLILIAGFGMVYGLYDDPAHLPWNINVMMLLGVIMSVVFVWI
                     YFGPYARFRRTTDRNTAAASIDRIRKLIGVNLVLGLVTVVVALLH"
     gene            complement(982..1611)
                     /gene="parA"
                     /locus_tag="VKQ27_04070"
     CDS             complement(982..1611)
                     /gene="parA"
                     /locus_tag="VKQ27_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HLY88137.1"
                     /translation="MAVVITVAQQKGGTGKTTLAANLAAAFAPSRRVALLDIDPQKSL
                     GRWHALRRARLQEAAALAFSDTSGWRLPAELDRLKRDHDLVLIDSPPHLDTDARVAVR
                     AADIVLVPIQPSLPDVWAAEGTLELAAAERRRAHLMFNRTPATSKLRDSIAADLGARG
                     LPLLRSALGNRAGFAHAFAAGLGVTEAAPRSTAAAELRALLDELRELTG"
     gene            complement(1665..>2348)
                     /locus_tag="VKQ27_04075"
     CDS             complement(1665..>2348)
                     /locus_tag="VKQ27_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HLY88138.1"
                     /translation="PREAVVERPDALTLAEAGSLGVPFVTAQEGFSRAGMPKPGDVVL
                     VMAVNGKVGQAAAQIAAMLGARVFGVTRRTEAFAHAHCAVRMIDGSSEDVAAVVRAET
                     DRHGADIVYNTVGSPYFAAANAAMAHAGRQIFIATIDRAVPFDIFTFYRGRHSYFGVD
                     SLALDASASAAMLRELLPGFASGALRPFPVNRSTTYPLDRAPEAYRAVHAIDRDRIVL
                     VPSLHIPER"
BASE COUNT          453 a          797 c          726 g          372 t
ORIGIN      
        1 gcaccgaatt gcgctcgttc gcgtccttgt aggcccagat gtgcacccat ttgttcagcg
       61 caccgaactc ggtgtacatg ccggcgacca gcggcgagaa cttctgcctc ccttcgatct
      121 tctccgacca acgctcgatc aggcccggaa tggacccggg ggccagcgtg tactggcgga
      181 tctcgaacaa cgggccaatc tggcgcgcct cgagcggcgg cgaaaacggt gccgggtaga
      241 agatctcgtt cttcatatcg accaggaact cgccgatcgg cggcggccag ccgccagcct
      301 tcatcgcggc gccgcgcacc gtcgtgcgct gctcgaaatt gtcatacgcc cacacgtgga
      361 tgaactggtt cagttggccc acttcgctgt gccacacggc cacgagctgc ttgccgtgct
      421 tctcgcggtt cggcaggtgc tcgccaaagc gcttttccac ctcggcgatg gtgcctggcc
      481 gcacagtgta ggtgcgcatt tcgatgatca tcaggatccc ctccctggga cacgcccacg
      541 cctagtgtag caacgccacg acgactgtca cgaggcccag cacgaggttc acgccgatca
      601 gcttgcgaat gcggtcgatg ctggcagccg cggtgttgcg atcggtggtg cggcggaagc
      661 gtgcatacgg accaaagtag atccacacga acaccacact catgatcacg ccaagcagca
      721 tcatgacatt gatgttccac ggcaggtgcg caggatcatc gtagagtcca tacaccatgc
      781 cgaatcccgc gatcaggatc agcggcacga cgtgccagat caccaggaag aagcggcgaa
      841 acacctgcgt gtggagagca atccgctgga tcggctccag caccgacagg ctcggtcgca
      901 gcaccacata ggcgaagaac atgccgccaa cccagatcac tgcgcacagc acgtgcacgg
      961 ccttcaccca gtcccagacg ttcatcccgt cagctcccgc agttcatcga gcagcgcgcg
     1021 cagctccgcg gcggcggtgg agcgcggcgc ggcctcggtg acgccaaggc cggcagcaaa
     1081 ggcatgcgcg aagccggcgc ggttgccaag ggccgaccgc aacagcggca gtccgcgcgc
     1141 ccccaggtcg gccgcaatgc tgtcacggag cttcgaggtc gcgggcgtgc gattgaacat
     1201 cagatgcgcc cgccggcgct cggccgcggc aagttccaag gttccctcgg cggcccagac
     1261 atcgggcaaa ctgggctgta tcggcacgag cacgatgtcc gcggcgcgca ccgcaacacg
     1321 tgcatcggtg tcgagatgcg gcggactgtc gatcagcacc aagtcgtggt cgcgcttcag
     1381 acgatcgagt tcggcgggaa ggcgccagcc ggacgtgtcg gagaaggcga gcgccgcggc
     1441 ctcttgcagg cgggcgcggc gcagcgcgtg ccagcggcca aggctcttct gcggatcgat
     1501 gtcgagtagt gcgacgcggc gggacggcgc gaacgcggcg gcgagattgg cggcgagcgt
     1561 ggtcttgcca gtgccgccct tctgctgcgc gacggtgata accacggcca tgactgcccc
     1621 ctgtcattgc gagaagcgta agcgacgaag caatctcggc cgagtcatcg ctcgggtata
     1681 tggagtgaag gtaccagcac aatgcggtcc cggtcgatcg catgcacagc gcggtaagcc
     1741 tcgggagccc ggtcaagtgg atacgttgtg ctcctattca ccgggaacgg ccttagcgcg
     1801 ccacttgcaa atcccggcaa cagctcacgc agcatcgcag cggaggcgct cgcgtccagc
     1861 gccagcgaat cgacgccgaa atagctgtgc cgcccacgat agaacgtgaa gatatcgaac
     1921 ggcaccgcgc gatcgattgt ggcgatgaag atctgtctgc cggcatgcgc catcgccgca
     1981 ttggccgcag cgaaataggg actgccgacc gtattgtaga cgatatccgc cccgtgcctg
     2041 tcggtctccg ctcgcacaac cgcggcgaca tcctcgctcg acccatcgat catccgcaca
     2101 gcacaatgtg cgtgtgcaaa cgcctcagta cgccgcgtga ctccaaacac ccgagcaccg
     2161 agcatcgcgg caatctgcgc cgccgcctgc cccaccttgc cgttcaccgc catcaccagc
     2221 acgacatcgc cgggcttcgg catacccgcc cgcgaaaacc cctcctgcgc cgtgacgaac
     2281 ggcacgccga gtgatcccgc ctctgccaaa gtcagcgcat cgggccgttc caccaccgct
     2341 tcgcgcgg
//
LOCUS       DATGEM010000129        16471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_992711, whole genome shotgun sequence.
ACCESSION   DATGEM010000129 DATGEM010000000
VERSION     DATGEM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992711"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VKQ27_04080"
     CDS             complement(<1..626)
                     /locus_tag="VKQ27_04080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HLY88139.1"
                     /translation="MPSLKALRNRIAATKATQKITKAMQMVAASKLRRAQATAEAARP
                     YAASMEKVLGNIAAGVGSAETAPRLLRGTGSDNVHLLLVCTAERGLCGPFNTAIVRAA
                     RERAIALSAEGKEVKFFCVGRKGYDQLRRQFSKQIVELVDLRGARILHFEHAEGIAEK
                     VIARFNNFEFDVCTLFFSRFRSVIAQIPTAQQIIPPLFDTKATDTLGGA"
     gene            complement(738..2267)
                     /gene="atpA"
                     /locus_tag="VKQ27_04085"
     CDS             complement(738..2267)
                     /gene="atpA"
                     /locus_tag="VKQ27_04085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLY88140.1"
                     /translation="MDIRAAEISAILKEQIKNFGQEAEVSEVGQVLSVGDGIAHVYGL
                     DNVQSGEMVEFESGVRGMAINLESDNVGIVIFGGDRDIKEGQTVKRTRAIVEVPVGKG
                     LLGRVVDALGDPIDGKGPIAADHRARVDVKAPGIIPRKSVHEPMATGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAIALDTILNQKPLNASSDEKIKLYCVYVAIGQKRSTVAQFVK
                     VLEENGALDYSIVIAATASDPAPMQFLAPFSGCAMGEYFRDNGMHAVIIYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDDNGAGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQTKAMKKVAGRIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLNRGSRLTELLKQPQFSPLKMEEQVCVVYTGVN
                     GYLDPLPVERVRAFEDGLLGLLRGQHADILDAIRSSRDLDDATAAKLKGVVDSYAKTF
                     A"
     gene            complement(2267..2827)
                     /locus_tag="VKQ27_04090"
     CDS             complement(2267..2827)
                     /locus_tag="VKQ27_04090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HLY88141.1"
                     /translation="MAAAETIVSGMAGRYATALFELAREANSIDLVKAGLDRFDGLIA
                     ESPDLLRLVRSPVFSADEQLAALSAVLQRAGIGGLAEKFLKLVTTNRRLFSIRDMIKG
                     FGELVAAHKGQATAEVTVAEPLKDGHVAALKSALKAVSGKDVDLDVKVDAALLGGLVV
                     KLGSRMVDTSLRTKLNAIRHAMKEAR"
     gene            complement(2848..3366)
                     /locus_tag="VKQ27_04095"
     CDS             complement(2848..3366)
                     /locus_tag="VKQ27_04095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HLY88142.1"
                     /translation="MPGPEAWVALAFVCFLGVLVYLGAHRKVVDRLDRHQARIKGELH
                     EAVRLREDAQALLSEVQRNALAAEGEAEAVIASAKAEAEGLAAEAKLRMEDLVARRTR
                     LAEMKIAQAEAQALADVRGAAADAAVAVAERILKDAGGSDVRNALLSHGIRDVRSRLN
                     GGHAKIALDAAE"
     gene            complement(3353..3880)
                     /locus_tag="VKQ27_04100"
     CDS             complement(3353..3880)
                     /locus_tag="VKQ27_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HLY88143.1"
                     /translation="MAGQPSFPPFQTENFASQLFWLGLTFVLLYVLMSRIALPRIGAI
                     MAERSKRIAEHVAAAQSLKEQADAAHAARDRALAAARSRAQTLASRERERLVAADHEA
                     GERLAARLRERLGAANSSIDAARSTAMGNVWTVAAELAPAIVQRLTGKTPTGPDVAVA
                     MAETRKPGGSVHARS"
     gene            complement(3921..4148)
                     /locus_tag="VKQ27_04105"
     CDS             complement(3921..4148)
                     /locus_tag="VKQ27_04105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HLY88144.1"
                     /translation="MDPTAAKYIGAGIACIGMGGAGVGVGIIFGNYLAAALRNPSAAQ
                     GQFGNLIFGFAITEALGIFSLLIALLLLFGI"
     gene            complement(4184..4933)
                     /locus_tag="VKQ27_04110"
     CDS             complement(4184..4933)
                     /locus_tag="VKQ27_04110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLY88145.1"
                     /translation="MMDPIHQFQIKNYFPFGTIAGHEIAFTNSALYMLATVALVSMLL
                     IGGTASRAIIPGRLQSAAELSYEFVANTLRSSTGPEGMRFFPFVFTLFMFVLTANFIG
                     LIPYSFTVTSQIVITATLALLVFFIVVGYGLWRNGVRFLRLFVPKGVPVYILPAIVAI
                     EILSFLSRPISHSVRLFANMLAGHITLQVFAGFITMLAGWGLLGWFGAILPFLMVVML
                     IALEILVAFLQAYVFAILTCIYLNDAIHPGH"
     gene            complement(4930..6183)
                     /locus_tag="VKQ27_04115"
     CDS             complement(4930..6183)
                     /locus_tag="VKQ27_04115"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HLY88146.1"
                     /translation="METVLVVNAGSSSVKFQVFAIEGRGLRRLVKGQMEGIRTRPKLR
                     AQDTDGKSLIEQVYAPEQVPDVAAALRTAGAWLRDAHNLEPIAVGHRVVHGGPEYHQP
                     MLIDAEVVDRLESYVSLAPLHQPNNLAPIRALLAQYPDLPQIACFDTAFHRGHDPVAD
                     HYAIPEHFYRDGVRRYGFHGLSYEYVASHLRSVAPGIADGRVIVGHLGSGASMCALSN
                     GRSVESTMGFTALDGLPMGTRSGQIDPGVVLYLIDQAKMSSEEVRSLLYGACGLIGLS
                     GLSSDVRELDNSPDPRARFALEYFAYRVGLYAGQLAAALGGLDAFVFTAGIGENSASM
                     RSRIVERLGWLGAILDPQANETGQFAIAKPTSRIGLFVIPTDEELMIARHTLALLGER
                     KIGTDLGTTAAQRPAATGTLRRRAS"
     gene            complement(6186..7181)
                     /locus_tag="VKQ27_04120"
     CDS             complement(6186..7181)
                     /locus_tag="VKQ27_04120"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HLY88147.1"
                     /translation="MLGEDSGEIGSIAALAPVGDLPVSKYERLIARAKQLRPPNTVVA
                     YPCEETALRGPLEAAEAGIIRPILVGSAAAISAIARKHGLDLDGHQIVDVPEPEAAAA
                     KAVALIHEGKAELLMKGSLHTDELMREVTSSKTGLRTARRISHVFIMDVPHHPETLFI
                     TDAAINIFPDLDTKRDIVQNAVDFFTQVGLGTPRVAILSAVENVTSKIPSTIEAAALC
                     KMAERGQITGALLDGPLAFDNAISPEAARIKGIKSEVAGRAQILVVPDLEAGNMLAKN
                     LSFLARADSAGVVLGARVPIILTSRADSVRARMASCAAASLYVDARRRKAPLPTV"
     gene            complement(7217..9466)
                     /locus_tag="VKQ27_04125"
     CDS             complement(7217..9466)
                     /locus_tag="VKQ27_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HLY88148.1"
                     /translation="MDAAKTQSPAAVSPTLGDVAPLLPNSVSSFTDYAFDAAQRSILF
                     LDVLRQRGNQYREHLAETVPHVLDYGAELVVDGRTLEHPVNYCLVRIIPPPGRVIDLK
                     RRPFVVVDPRAGHGPGIGGFKADSEIGVIFAAGHPCYFVGFLPDPMPGQTIEHVALAE
                     AIFLEKVISSHPDADGKPCVVGNCQAGWAIMMLAAIRPELFGPIIIAGSPLSYWAGVR
                     GANPMRYTGGLLGGSWLTALVSDLGHGKFDGAWLVQNFENQNPANTLWQKPYNLYAKV
                     DTEAPRYLDFERWWGGHTNLNADEIQFIVDELFIGNNLSAAKIRTSDGVAVDLRNIRT
                     PIVVFCSKGDNITPPQQALGWILDLYESVDEIRSYGQTIVYTIHESVGHLGIFVSGGV
                     AKKEHGELSTNIDLIDTLPPGLYEAIIENKTEQTSNPELASGNWVMRCEARTLDDLRA
                     LGGNDLADERRFAAAARLSELNLAMYRAFVQPAVRAGMNSSFADLMWRCHPLRLQYQF
                     FSDANPAVAPIGEIAEEVRKARKPVAPDNPFLKIQQTMSRNIVAALDGWRKATENIAE
                     SAFISFYGSPILQTMLGIQSDTRRSARKPDKSLLHRELLAKRIAELKSRIAVGGLPAA
                     VIRALLYAGMGRKAIDERGFEAVRRIRQAHGDMSLSAFKALVREQFSILLIDSDAALA
                     VIPSMLPPDAETRKVALGLIRTVLGARGEISGDDRKRLDEVTRLFELDGKALPALESS
                     APKRTEIAA"
     gene            9762..10871
                     /locus_tag="VKQ27_04130"
     CDS             9762..10871
                     /locus_tag="VKQ27_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLY88149.1"
                     /translation="MDALTQFTDPEPLNKNTMDALVRLSILALLVYWAALLIQPFLVL
                     ITWSIVLVIVLYPAYSFAIRRLHLPPAVAAGVLVVCCLVILLGPATWLGLSLGTSIRW
                     FAQRLASGELAIPPPFEAVRKWPLIGAKTYELWSLASSNFTEAFAKIGPGLRPLSTRL
                     LVLASSAGLSMFKFIMSVIIAGFLFKPGPSMVRSAGVVFRRLTTEHGDEYVQLVGVTI
                     RNLARGVIGVALLQSLLVGIGLIVAGVPAAGLISLAVLILAIIQIGSAIVIIPLVVWS
                     FFAMDTPAAILFSIYMVPVSLVDNLLRPIIMARGLKTPMLVILIGLLGGVWVHGLIGL
                     FVGPIVLAIGWELVGLWIWQRSGESDSDATLALKE"
     gene            complement(11016..11411)
                     /locus_tag="VKQ27_04135"
     CDS             complement(11016..11411)
                     /locus_tag="VKQ27_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88150.1"
                     /translation="MTASLAIATLVASTAAEAQIGFGRPGIGVGRVGSGFGFRPGLGF
                     GRAGLGWSGWRGGLGYGYGRWGYGGYGYGAAALAGAAIGYGLASPYYGYGYGYGYGYG
                     YPHYYGYAPAATYYDYSPGYYSGGCTCPY"
     gene            complement(11574..12047)
                     /locus_tag="VKQ27_04140"
     CDS             complement(11574..12047)
                     /locus_tag="VKQ27_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88151.1"
                     /translation="MNLSTEVKIGSQVIALPTAKQLKQQIALKEAQRASSAVREKVAA
                     DAEKRALMEKLSKPSGVSDDERLERAIAIINRAANNGASEVEVARFPNTLCTDRGRAI
                     NQQEGGWETTLTGVPKELYDFWQKYLRANGFKLRVQIVDFPGGVPGDIGMTLSWG"
     gene            complement(12136..12588)
                     /locus_tag="VKQ27_04145"
     CDS             complement(12136..12588)
                     /locus_tag="VKQ27_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY88152.1"
                     /translation="MHHEMRSYPSDSFSTVPLLPDRGQTPALQPPRQPPITWQVRRAE
                     EYIETHWNQPITIANIARATAASARSIFYHFKSSRGQSPMCFLKQVRLEHAREMLASS
                     GIGRSVTEIAIDCGFGNLGHFAGDYLKRFGERPSDTLKRSKYELALRH"
     gene            complement(13055..13786)
                     /locus_tag="VKQ27_04150"
     CDS             complement(13055..13786)
                     /locus_tag="VKQ27_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY88153.1"
                     /translation="MEIAQMQIKGRFGLHRDQTPARMGEPSEISGVVMGTLAGDEPIV
                     FVVDDSADVRDGLKELLESVGLRCKAFDSPKEFLNRAPEVGPNCLILDVRLPEISGLD
                     VQAELARGPRNIPIIIITGHGDIPMSVRAMKAGAVTFLTKPVREQDLLDAVYGAIETD
                     RVRLNSELKNRELLARYESLSAREREILPLITAGLLNKQIAAEVGLSEVTVKVHRHKL
                     MAKLNAKRLPDLVRMADELGIKRGH"
     gene            complement(13804..14835)
                     /locus_tag="VKQ27_04155"
     CDS             complement(13804..14835)
                     /locus_tag="VKQ27_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HLY88154.1"
                     /translation="MQADQSADKIGAVPTAAGGIARAAYTRAIISLPDAALLAKRAGI
                     SLKQLNDPMFRIAVGNQITFLNLVAERLPDEFLGFHLASKVDLRELGLLYYVQASSDS
                     LGEALRRTQRYCSIQNEGVLIKYREGDPICVSFHYNAVRRLSDRHQIEFFVTMVVRLC
                     RQLVAQRLNPIRVKFVHRRTEALGDMAACFGCEFVFGSEVDEIEYAPTSARLPIASAD
                     AYLNRLLARYCEEALSKRRVKARSWRVAVENTLVPLLPHGQGRLSEVAARLGVSVRTL
                     ERRLAAESVSFVDVLDGLRCELAKRYLQEPSLPISEIAWLLGYADPSAFTHSFRRWTG
                     KPPTQLRAA"
     gene            14953..15321
                     /locus_tag="VKQ27_04160"
     CDS             14953..15321
                     /locus_tag="VKQ27_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88155.1"
                     /translation="MHQPASMRRTMMFLLNRLMSNSKRHKHLERLIVQSYVWVLRAPR
                     AIDGQKVASLSRHGLYEVRLLEPSEVAQGNDLPFWVELVDNKRNLCLDSYAGDDIEET
                     AIAVEHLISRAELLDQGSES"
     gene            15788..>16471
                     /locus_tag="VKQ27_04165"
     CDS             15788..>16471
                     /locus_tag="VKQ27_04165"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HLY88156.1"
                     /translation="MLLYLLAVPAFAQNSQPLPGASSGQGLLSGPQLEALVAPIALYP
                     DPLLSEIMMASTYPLEVVAADRWAKSNANLQGDQLKAAVEQQPWDQSVKSLVATASVL
                     DMMSTKLDWTQQLGDAVLAQQSDVMDAVQRLRQRAQNTGKLKSTPQQTVNAIPAEAGA
                     APAPSGAAPGAGGAPVVQGGGQVIAIEPTDPDTLYVPYYDPAVVYGEWPYSDYPPYYF
                     GYPGYIGAGI"
BASE COUNT         3148 a         5056 c         5003 g         3264 t
ORIGIN      
        1 gcgccgccga gcgtatcggt ggccttggtg tcgaacagcg gcggaatgat ctgttgcgcg
       61 gtcgggatct gggcgatcac cgagcggaag cgcgagaaga acagggtgca gacgtcgaat
      121 tcgaagttgt tgaagcgcgc gatcaccttc tcggcgatgc cctcggcgtg ctcgaagtgc
      181 aggatgcggg cgccgcgcag atcgaccagc tcgacgatct gcttgctgaa ctggcggcgc
      241 agctggtcgt agcccttgcg gccgacgcag aagaacttga cctccttgcc ttcggccgaa
      301 agtgcaatgg cccgctcgcg cgcagcgcgc acgatagcgg tattgaacgg gccgcacagg
      361 ccgcgttcgg cggtgcagac cagaagcaga tggacattgt cgctgccggt gccgcggagc
      421 agccgcggcg ccgtctcggc gctgccgacg ccggcggcga tgttgcccaa aaccttctcc
      481 atgctggccg catagggccg cgccgcctcg gccgtcgcct gggcgcggcg cagctttgac
      541 gccgcgacca tctgcatggc cttggtgatt ttctgcgtcg ccttggtggc ggcgatgcgg
      601 ttgcgcaggg ctttgaggct cggcatggcg gtcaggagcc agtgatcagt aatcggtcat
      661 cagggaccaa gtgccaggag tcggagtcag gtgcgatttc gacattctga ttaccaaccc
      721 ctgacctctg attcctgtca cgcgaatgtc ttggcgtagc tgtcgaccac gcccttgagc
      781 ttggcggcgg tggcgtcgtc gaggtcgcgg gagctgcgga tggcgtcgag gatgtcggcg
      841 tgctggccgc gcagcaagcc gaggaggccg tcctcgaagg cgcgcacccg ctcgaccggc
      901 agcggatcga gatagccgtt gacgcccgta tagacgacgc agacctgctc ctccatcttg
      961 agcggcgaga actgcggctg cttgagaagc tcggtgagcc gcgagccgcg attgagcagc
     1021 cgctgcgtgg tggcgtcgag atcggagccg aactgcgcaa acgccgccat ctcgcggtat
     1081 tgcgcaagct cacccttgat gcggcccgcg accttcttca tcgccttggt ctgtgccgcc
     1141 gagccgaccc gcgacaccga caggccgacg ttcaccgcgg ggcggatgcc ctgatagaac
     1201 aagtcggttt cgagaaagat ctggccgtcg gtgatggaga tgacattggt cggaatatag
     1261 gccgacacgt cgttggcctg ggtctcgatc accggcagcg cggtcagcga gccggcgccg
     1321 ttgtcgtcgt tcatcttggc ggcgcgctcc aagagccgcg agtgcaggta gaacacgtcg
     1381 cccggatagg cctcgcggcc cggcggccgg cgcaaaagca gcgacatctg ccgatacgcc
     1441 acggcctgct tggacagatc gtcgtagatg atcaccgcgt gcatgccgtt gtcgcggaag
     1501 tattcgccca tggcgcagcc ggaaaacggc gccaaaaact gcatcggcgc cggatcggac
     1561 gcggtggcgg cgatcacgat cgagtagtcg agcgcgccgt tctcctccaa taccttgacg
     1621 aactgcgcca ccgtcgagcg cttctggccg atcgccacat agacgcagta cagcttgatc
     1681 ttctcgtcgc tcgaggcgtt gagcggcttc tggttcaaga tggtgtcgag cgcgatcgcg
     1741 gtcttgccgg tctggcggtc gccgatgatc agctcgcgct ggccgcggcc gatcgggatc
     1801 agcgcgtcga tggctttcag ccccgtcgcc atcggctcgt gcaccgactt gcgcggaatg
     1861 atgcccggcg ccttgacgtc gacccgggcg cggtggtcgg ccgcaatcgg ccccttgccg
     1921 tcgatcggat cgccgagcgc atcgaccacg cggccgagca aacccttgcc gaccggcacc
     1981 tcgacgatgg cgcgggtgcg cttgacggtc tggccctcct tgatgtcgcg gtcaccgccg
     2041 aaaatgacaa tgccgacatt gtcgctctcg agattgatcg ccatgccgcg aacgccgctc
     2101 tcgaactcga ccatctcgcc cgactgcacg ttgtcgaggc cgtaaacgtg cgcaatgccg
     2161 tcgccaaccg acagcacctg gccgacctcg gagacttcgg cctcctggcc gaaattcttg
     2221 atctgctcct tgagaatggc ggaaatctcc gccgcgcgga tatccattag cgggcctctt
     2281 tcatcgcgtg cctgattgca ttgagcttcg tccgcagcga ggtgtcgacc atccggctgc
     2341 cgagcttgac cacgagcccg cccaggaggg cggcatccac tttcacatcg agatcgacgt
     2401 ccttgccgct gaccgccttg agcgcgcttt tgagcgccgc aacgtggccg tccttgagcg
     2461 gctccgcaac cgtgacctcg gcggtcgcct gtcccttgtg ggcggccacc agctcgccga
     2521 aacctttgat catgtcgcgg atcgaaaaca gccgccggtt cgtcgtcacc agcttgagaa
     2581 atttctccgc caggccgcca atgccggcgc gctgcagcac cgccgacagc gccgccagct
     2641 gctcgtcggc cgaaaacacc gggctgcgaa ccagccgcag caggtcaggg ctctcagcaa
     2701 tcagcccgtc gaaacggtcc agccccgcct tgaccaaatc gatcgaattc gcctcccgcg
     2761 ccagctcaaa caaagccgtc gcatagcgac ccgccatgcc cgatacaatg gtctctgctg
     2821 ccgccaccgt gctgaacccc tgatcttcta ctccgcggca tccagggcga tcttcgcatg
     2881 acctccattc agccttgagc ggacgtctct aatgccgtga gaaagcagcg cgtttctcac
     2941 gtcgctgccg cctgcgtctt tgagaattct ctcggcgaca gcaacggccg cgtccgccgc
     3001 ggctccacgg acgtcggcga gcgcctgcgc ctcggcctgg gcgattttca tctccgctaa
     3061 cctggtacgg cgcgcgacca aatcctccat gcgtagcttt gcttcggcgg cgagaccctc
     3121 cgcctcggct ttggcgctgg cgataacggc ctcggcctcg ccttcggcgg caagcgcatt
     3181 gcgctgcact tcgctcagca acgcttgggc gtcctctcgc agtcgaaccg cttcgtgcag
     3241 ttcgcccttg atgcgggcct gatgccggtc gagcctgtcg acgaccttgc gatgggcgcc
     3301 gagatagacg agcaccccga ggaagcagac aaacgccaaa gcaacccaag cttcaggacc
     3361 gggcatggac agaacctccg ggcttgcgtg tttcggccat ggcgacggca acatcgggtc
     3421 cggtgggcgt tttgccggtg agccgctgca cgatggccgg agcgagctct gccgcgacag
     3481 tccagacatt gcccatggcg gtgctacgtg ccgcatcgat ggagctattg gcagcgccga
     3541 gtcgttcgcg caatcgcgcc gcgaggcgct cgccggcctc gtgatcggcg gcgacgagcc
     3601 gctcgcgttc acggctggcc agcgtctgcg cgcggctgcg cgcggccgcg agggcccggt
     3661 cgcgggcggc atgggcggcg tccgcctgct ccttcaggct ctgagcggcg gcgacatgct
     3721 cggcaattcg tttgctgcgc tccgccatga tcgcgccgat gcgcggcagg gctattctgg
     3781 acatcagcac atatagcagc acgaaagtca gtccgagcca gaacagctgc gaagcgaagt
     3841 tttccgtctg aaacggcgga aacgacggct ggcccgccac agccccggta tgagcagtcg
     3901 tctcagccat ggtgtcctcg tcagattccg aacaagagca aaagcgcgat cagcagcgaa
     3961 aagatgccga gcgcctcggt gatggcaaag ccgaaaatca gattgccgaa ctggccctgc
     4021 gccgccgaag ggttacgcag cgcggccgcc agataattgc cgaaaataat tccgacgccg
     4081 acgccggcac cccccatgcc gatgcaggcg atgccggctc cgatatattt cgcggcagtc
     4141 gggtccatga gcagctcctt tcccgttcac aagcaacggg ctctcaatgg cccgggtgta
     4201 ttgcatcgtt gagatagatg caggtgagaa tggcgaacac gtaggcctgc agaaacgcga
     4261 ccaagatttc gagcgcgatc agcatcacga ccatgaggaa cggtaagatc gcgccgaacc
     4321 agccgagcag accccatccg gccagcatgg tgatgaagcc ggcaaatacc tgcagcgtta
     4381 tatggccggc cagcatgttg gcgaagagac gcacgctgtg cgagatcggt cgcgacagaa
     4441 acgacaaaat ctcgatcgct acgatcgccg gaaggatgta gaccggcact cccttgggca
     4501 cgaacaaccg caaaaagcgc acgccgttcc gccacaggcc gtagccgacg acgatgaaga
     4561 acaccagcag cgccagcgtc gccgtgatga cgatttggct ggtaacagta aacgaatagg
     4621 gaatgagccc tataaagttg gcggtcagga cgaacatgaa cagggtgaac acgaaaggaa
     4681 agaatctcat tccttccggc ccggtcgagc tgcgcaacgt gttggcgaca aactcgtagg
     4741 acagttcggc ggccgattgc agccgccctg ggataatggc ccgcgacgcg gttccgccga
     4801 tcaaaagcat cgaaacgagc gcgactgtgg ccagcatgta caaggcagag ttggtgaacg
     4861 cgatttcgtg gccggcgatc gtgccaaagg ggaaataatt cttgatctga aattgatgga
     4921 tcggatccat catgaagccc tccggcgcag cgtgccggtc gcagccggcc gttgcgccgc
     4981 tgtcgtgccg agatccgtgc cgatcttgcg ctcgccgagc agcgcaagcg tatggcgtgc
     5041 gatcatcagt tcttcatcgg ttggaatgac gaaaagcccg atccggctcg tcggcttcgc
     5101 aatcgcaaac tggcccgtct cattggcctg cgggtccaat atggcgccga gccagccaag
     5161 tctttcgacg atgcgagatc gcatggaggc cgagttctca ccgatgccag cggtaaagac
     5221 gaaggcgtcg aggccgccaa gcgctgccgc gagctgacct gcatagaggc ccactcggta
     5281 ggcgaaatac tccaaggcaa agcgcgcgcg cgggtccggg ctgttatcga gttcgcggac
     5341 gtcgctgctc agccccgaaa gcccgatcag accgcaggcg ccgtagagca gggatcgcac
     5401 ctcttcgctg ctcatcttcg cttgatcgat gaggtaaagc acgactccag gatctatctg
     5461 acccgagcgt gtgcccatcg gcaatccatc cagcgccgtg aagcccatcg tgctttcgac
     5521 gctgcggccg ttcgacaatg cgcacatcga ggccccgctg cccaggtggc ctacgatcac
     5581 tcgaccgtcg gcgatacccg gagcgaccga tcgcagatgc gacgcaacgt attcgtatga
     5641 cagcccgtgg aatccataac ggcggacccc gtcgcggtag aagtgctccg gaattgcgta
     5701 atgatcggcg accggatcat gtccgcgatg aaacgcggtg tcgaagcagg cgatttgcgg
     5761 cagatccgga tactgcgcaa gcagggcgcg gatcggcgcc agattgttgg gttggtgaag
     5821 cggcgcgagc gatacgtaac tttcgagccg gtcaaccacc tcggcgtcga tcaacatggg
     5881 ctgatggtat tcggggccgc cgtggacgac gcgatgcccg accgcgatcg gttcaagatt
     5941 atgtgcgtcg cgcagccacg ctccggcggt tcttaaagcg gccgcgacgt ccgggacctg
     6001 ctccggagca tagacttgct cgatcagcga ttttccatcg gtgtcctgag cgcgtagttt
     6061 cggccgggtc cgaatgccct ccatctgacc cttgaccagc cgtctgagac cgcggccttc
     6121 aatggcaaac acctgaaatt tgacgctcga agatccggca ttgacgacca ggactgtttc
     6181 catgctcata ccgtcggtaa aggcgccttc cgtcgtcgcg cgtccacgta gagagatgca
     6241 gctgcgcaag aggccatccg ggcgcgcacc gaatcggcgc gcgatgtcag gatgatcggc
     6301 acgcgtgcgc caagcacgac gccggcggag tccgctcgcg ccagaaatga caggttcttc
     6361 gccagcatgt tgccggcttc caaatccggc accacgagaa tttgcgcgcg accggcgacc
     6421 tcggatttga tgcctttgat gcgcgccgcc tcgggggaga tggcgttgtc gaacgcgagc
     6481 ggcccatcca ggagcgcgcc ggtgatctgg ccgcgctcgg ccatcttgca aagtgcggca
     6541 gcctcgatag tcgagggaat cttcgatgtc acgttctcga ccgccgaaag gatcgcgacc
     6601 cgaggcgtgc cgaggccgac ctgggtgaag aaatcgaccg cgttctgaac aatatcgcgc
     6661 ttggtgtcga gatccggaaa gatattgatg gcagcgtcgg tgatgaacag ggtttccgga
     6721 tggtgcggca cgtccatgat gaagacgtgg ctaattcgtc ttgcggtgcg caatccggtt
     6781 ttgctggagg tgacctcgcg catcagttcg tcggtgtgca ggctgccctt catgagaagc
     6841 tcggccttgc cttcgtggat gagggcaact gccttcgcgg ccgccgcctc gggttcgggc
     6901 acgtcgacga tttggtgacc gtcgaggtcg agcccgtgtt tgcgggcgat tgcagaaatg
     6961 gcagcggcgg atccaacaag gatcggccgg attatgccgg cttccgccgc ctccaacgga
     7021 ccgcgtagcg ccgtctcttc gcagggatag gcaaccaccg tgttcggcgg cctcagctgc
     7081 ttggctcgag ctatgagccg ctcgtatttc gaaacgggca ggtcacccac gggagcaagc
     7141 gccgcgatac tgccgatctc gccactgtct tcgccgagca tgattgagcc tccatcggct
     7201 gagcaattca gcacgtctag gctgcgattt cggtccgctt cggcgcgctg ctttccaggg
     7261 caggaagcgc tttgccgtcc aactcgaaca gccgcgtcac ctcgtccagc cgtttccgat
     7321 cgtcgccgga aatctcgcct cgtgcgccaa gcaccgtcct gatcaatccc aacgccactt
     7381 tccgtgtctc tgcatcgggc ggcagcatgg atgggattac ggcgagcgcc gcgtcgctgt
     7441 cgatgagcag aatactgaac tgctcgcgca ccagcgcttt gaaagcggac agcgacatgt
     7501 cgccgtgcgc ctgtcgaatg cgccggaccg cctcaaagcc cctttcatcg atcgcttttc
     7561 tgcccatccc ggcataaagg agggcgcgaa tgacggctgc cggcagtccg ccgaccgcaa
     7621 tgcgagactt cagctcggca atgcgcttcg caagcagctc gcggtgcaac aggcttttgt
     7681 ccggcttccg cgcggatcgc cttgtgtctg actggatacc gagcatcgtt tgaagaattg
     7741 gcgagccgta aaacgagata aacgcgcttt ccgcgatgtt ctcggtcgct tttcgccagc
     7801 cgtcgagcgc cgcaacgatg ttgcgcgaca tggtctgttg aattttcagg aacggattat
     7861 cgggcgcaac gggcttgcga gccttgcgaa cttcctcggc tatttcgccg atcggtgcca
     7921 ccgccggatt cgcgtccgaa aaaaactggt attgcaggcg caacgggtgg cagcgccaca
     7981 tcaaatccgc aaatgatgaa ttcattccgg cgcgaacagc cggctgcacg aaggcgcggt
     8041 acatggccag attgagctcg gacaggcgtg ccgcagcggc aaaccgccgc tcgtcggcca
     8101 aatcgttgcc gcccagcgcc cgaaggtcgt ccaatgtcct tgcttcgcat cgcatcaccc
     8161 agtttccaga ggccagctcc ggattggatg tttgctcggt cttgttttcg attatggctt
     8221 catacaagcc cggcggcagc gtatcgatga ggtctatgtt tgtcgaaagt tcgccgtgtt
     8281 ctttcttggc tacgccgccg gacacgaaaa tccctaaatg accgacgctt tcgtggatgg
     8341 tgtagacgat cgtttgaccg taggaacgaa tttcatcgac gctctcatag aggtccagga
     8401 tccatccaag ggcctgctgc ggtggcgtga tgttgtcgcc ctttgaacaa aacacgacaa
     8461 tgggcgtgcg gatattgcgt aagtcgaccg cgacgccgtc cgatgtgcgg atttttgccg
     8521 cagaaaggtt gttgcctatg aacagttcat cgacgatgaa ttggatttcg tcggcgttca
     8581 aattggtatg gccgccccac caacgctcga aatcgaggta gcggggcgct tcggtgtcga
     8641 ctttcgcata aagattgtac ggcttttgcc agagcgtatt tgccgggttc tgattctcga
     8701 agttttgaac cagccacgca ccgtcgaatt tcccatgacc gagatcgctg acgagcgccg
     8761 tgagccagct gccgccgagc aatccgccgg tatagcgcat gggattcgcg ccccgcacgc
     8821 cagcccaata tgaaagcggc gagcccgcta tgatgatggg tccgaacagc tccgggcgaa
     8881 ttgcggccag catcatgatc gcccagccgg cctggcaatt gccgaccacg cacggcttgc
     8941 cgtcggcgtc ggggtgcgac gagatcacct tctcgaggaa gatcgcctcg gcgagcgcta
     9001 cgtgttcgat ggtctgcccc ggcatcggat cgggcaggaa gccgacgaaa taacagggat
     9061 gcccggctgc gaagatgacg ccgatctcac tgtcggcctt gaatccgccg ataccggggc
     9121 catgcccggc gcgcgggtcg acaaccacaa acggccgtcg cttgagatcg atgacgcggc
     9181 ccggaggagg aatgatcctg accaggcaat aattgactgg atgttcgagc gtgcgaccgt
     9241 cgacaaccaa ctcggcccca tagtcgagca catggggcac cgtttcggcc aaatgctcac
     9301 gatactgatt gccacgctgt cgcaggacgt cgagaaacag gatgctgcgc tgcgcggcat
     9361 cgaaagcgta atcggtaaag gacgaaacgc tattggggag cagtggtgcg acatcgccca
     9421 gcgtgggtga aacggcagct ggcgattgcg ttttcgctgc atccatcggt gtccgctccg
     9481 aggtaagcct cccagaagac tcagaaggcc gtgctgcccc gcagcaaaga aaccgctcct
     9541 atgggcgtcc gtccattacg ctccagaata gcgccccgac cctctcgggt cggcaatcaa
     9601 tcctttatat aacgcgtccg gttcctccgc cgaccggaag ctccctccaa actcctgcag
     9661 ttcttgagtt gcgacccgct tgccgttccg cacactggcc gattggccgg cgtagtctga
     9721 tcgcgccaca ccattgcccc ctggggtcgc acggctaagc gatggacgct ttaacccaat
     9781 tcaccgatcc ggaaccgttg aacaaaaaca cgatggacgc ccttgtgcgc ctgtcgatac
     9841 tggcgttgct tgtgtactgg gcggctcttc tcatccaacc cttcctggtt ctgatcacct
     9901 ggagcatcgt tctggtgatc gtgttatatc ctgcatattc cttcgccatc agaaggctcc
     9961 atttgccgcc tgccgtcgct gccggcgtcc ttgtcgtctg ctgcttggtg atattgcttg
    10021 gccccgccac atggctgggg ttgagcctgg ggacgagtat aaggtggttc gcgcagcggc
    10081 ttgcgtccgg cgaactcgcg attccgccgc ccttcgaagc ggtgaggaag tggccgctca
    10141 tcggcgcgaa gacctacgag ctgtggagct tggcttccag caatttcacc gaagcattcg
    10201 ccaagatcgg acctggatta aggccgctaa gtacgcgcct gctggttctt gccagcagcg
    10261 ccgggctcag catgttcaag ttcattatgt ccgtcattat tgcgggcttt ctgttcaagc
    10321 cggggccgtc gatggtgaga tcggccggcg tcgtttttcg acgccttacg accgagcacg
    10381 gcgatgaata tgtgcagctc gtcggagtaa ctataagaaa tttggctcgc ggcgtgattg
    10441 gcgtcgctct cctgcagtcg ctgctggtgg ggatcggcct catagtggcg ggggtgccgg
    10501 cggccgggct catcagtctg gcggtgctga ttctggcgat cattcagatc ggcagtgcaa
    10561 tcgtcatcat cccgcttgtc gtctggagct tctttgcgat ggatacgccc gctgccattt
    10621 tgttttcgat ctacatggtg ccggtcagcc tcgtcgacaa cttgctacgt ccgatcatca
    10681 tggcccgggg actgaagacg ccgatgctgg tcatcttgat cgggcttctc ggcggcgtgt
    10741 gggtgcatgg attgatcggc ctgtttgtcg ggccgattgt gctcgcgatt ggctgggaat
    10801 tggttgggct ttggatttgg cagcggagcg gtgagagcga ttcggacgcc accttggcgc
    10861 tgaaggaata aacagtacga gccgcgtcat cgtgcttgga ttcgaactcg gtccctcgcc
    10921 tgagcagttt tcggctgacc ccggccgcga atgctgatcc gcgcgctgcc acccggtcgg
    10981 atgacgacgc gcggaccagg ggcctctgtg ccgcctcaat acgggcaggt acagccgccg
    11041 gagtaatagc ccggagaata gtcgtagtag gtcgcagctg gagcatagcc gtagtaatgc
    11101 gggtacccat aaccatagcc gtatccgtac ccgtacccgt agtacgggct tgccagccca
    11161 tagccgatcg cggcgccggc aagcgctgca gcgccatagc cgtaaccgcc gtagccccag
    11221 cggccgtagc cgtaacccaa gccgccgcgc cagccggacc aaccgaggcc ggcacggcca
    11281 aagccgaggc ctggccggaa accgaaacct gaacctacgc ggcccacacc gatgcctgga
    11341 cggccgaagc cgatctgcgc ctcggcagcc gtcgaggcaa ccagcgttgc gatggccaga
    11401 cttgccgtca tgaataaagc gcgccttacg ttcccggcct tcagtgttct gaaaatcatt
    11461 tgcatggctc gagctccttt ctgctgcttt actctgtttg catgtttgga tcgcggctct
    11521 tcagttcaac gtaccttcgg tccagacacg attgcgtgcc tgccaaaaac tcctcaaccc
    11581 cagctcagcg tcatgccgat gtctccgggt acgccacccg gaaaatcgac gatctgcacc
    11641 ctcaatttga atccgttcgc gcgcaaatat ttctgccaaa aatcgtatag ctctttgggt
    11701 actccggtca gcgtggtttc ccagccgccc tcctgctggt tgatggcacg cccgcggtcg
    11761 gtgcagagcg tgttcggaaa acgcgccacc tcaacctcgc tcgcgccgtt gttcgcagct
    11821 cggttgatga tggcaatggc gcgctcgagc cgctcgtcgt ccgacacgcc cgagggcttc
    11881 gagagcttct ccatcaatgc tcttttttcc gcgtcggccg ccactttttc gcgtaccgcc
    11941 gagctggctc tctgcgcttc cttgagcgcg atctgctgct tgagctgttt ggcggtcggc
    12001 aacgcaatga cctggctgcc aatcttcacc tcggtactca ggttcatatc aggccccgtt
    12061 cgacggtccg aactgccgcc aaatcgattt caatggtcgt tttacttcgg tgctccacga
    12121 cggccggcag ccggcctaat gtctcagcgc gagctcgtat ttgctgcgct tgagcgtatc
    12181 ggacggacgt tctccgaacc gcttgagata gtcgccggca aaatgcccca agttgccaaa
    12241 accgcagtcg atggcgatct cagtcactga gcggccgata ccgctgcttg caagcatctc
    12301 cctggcatgc tcgagccgaa cctgtttcag aaaacacatt ggcgactggc cgcgactgct
    12361 cttgaagtga taaaaaatac tgcgcgcgct ggccgcggtg gcgcgcgcga tatttgcgat
    12421 cgttattggc tggttccagt gagtttcaat atactcctcg gcgcgccgca cctgccacgt
    12481 tatcggtggc tgccttggcg gctggagcgc cggcgtttgg ccgcgatcgg gcagcagagg
    12541 cacggtggaa aacgaatcgg acgggtaaga gcgcatctca tggtgcatcg ggccctcctc
    12601 attcacgcta acaacttggt ttgcgtttcc tcttcactcg cgcggcggcg ccgatggtca
    12661 ggtcgatgtc cccgttaggc ggtcgcacgg tcgtcgccgc ttgtcagttt cgtttccaat
    12721 gaaacgatca agagggtttg gatgtccatt acgccaatgc atttgcggcg tacttaattg
    12781 gctcgggcca atatactatc gcatataaaa aaacaggtcg tcggcgacgc acggatgctg
    12841 gttcgcgatt gacgcattac cgctgggcgc gcgtgcaagc ccaagtcgcg caaaagcgta
    12901 tcgcatgttg ctatgatgcg ttcgccttta ggcgaacgga ccaggcgggg agtgtatcgg
    12961 cggggttggc caggcgtctt ttagacccgt tcgtcggcgc gactctgcac tggagcgcgg
    13021 tcagtgaaat tggagcgcaa aaaaaccgga aagctcagtg gccgcgttta atgcccaatt
    13081 cgtcagccat tcttaccaaa tcaggcagcc gctttgcgtt caacttggcc atcagcttat
    13141 ggcgatgaac tttcaccgtg acttcactca atcccacctc ggcggcaatt tgcttgttta
    13201 gaagtccggc cgttatcaat ggtaatatct cgcgctctcg cgcgctgagg ctttcgtagc
    13261 gcgcaagtaa ttcacggttc tttaactccg aattcaggcg cacccgatcg gtttcgatcg
    13321 cgccgtaaac cgcgtccagc agatcttgct cacgaacggg ctttgtcaag aatgtcacag
    13381 cgccggcctt catggcccgc accgacatcg gaatatcgcc gtgcccggta attatgatta
    13441 tcggaatatt tcttggcccg cgcgccaact cggcttggac gtccaggccg ctgatttcgg
    13501 gcagccgcac gtcaagaata aggcagttcg ggccgacctc aggcgcgcgg ttgagaaatt
    13561 ccttgggaga gtcgaaggct ttgcatcgta agccgaccga ctcgagcaac tccttcaagc
    13621 cgtctcgtac gtctgcgctg tcgtcgacca caaagacgat cggctcgtcg cccgcaagag
    13681 tacccatgac aacccccgag atttcactcg gctctcccat tctggccggt gtttgatcac
    13741 gatgcaagcc gaacctgcct ttaatctgca tttgagctat ctccatgtgc cggtctcgac
    13801 aaatcaggcg gcacgcaatt gtgtaggagg ttttcccgtc caccgtctaa acgaatgagt
    13861 aaacgcgctc ggatcggcgt agcctagcag ccaagctatt tcggaaatcg gcaagctcgg
    13921 ctcttggaga tagcgctttg caagctcgca tctgagacca tcaagcacgt ccacaaacga
    13981 cacgctctct gcagcaaggc gtcgctcgag ggttcggaca ctgactccta ggcgagctgc
    14041 aacctccgat aatcgaccct ggccatgcgg cagcaatggc accagcgtat tttcgaccgc
    14101 cacccgccag gaccttgcct taactcgtcg cttcgaaagc gcctcttcgc agtatcttgc
    14161 cagcagcctg ttcagatagg cgtcggcgct ggctatgggc aatcgagcgc tcgtcggagc
    14221 gtactcgatc tcatcgacct cgctgccgaa gacgaattcg caaccgaagc atgccgccat
    14281 atcgcccagt gcttccgttc tgcggtgcac gaatttgaca cgtatcgggt tcaggcgctg
    14341 cgccaccagc tgccggcaca gcctcacaac cattgtgacg aagaactcga tctggtggcg
    14401 atcgctcagg cggcgcaccg cgttgtaatg gaaagatacg cagatcgggt cgccctcgcg
    14461 gtacttgatc aacacgcctt cgttttgaat ggagcaatac ctctgggtgc gccgcaaggc
    14521 ctcgccgagc gagtcggacg acgcctgaac gtagtaaagc aaacccaact cgcgaaggtc
    14581 taccttgctg gcaagatgaa atccgagaaa ctcgtccggc agccgttcag cgaccagatt
    14641 gagaaacgtg atttgatttc cgacggcaat gcgaaacatc ggatcgttca gctgcttgag
    14701 cgatattccg gcgcgtttcg ccagcagagc ggcgtctggc agcgaaataa tagcgcgcgt
    14761 ataggcggct cgggctattc cgccggccgc cgtcggtaca gcaccgattt tatccgcact
    14821 ctgatctgct tgcatttcgt tgaacctgtt tggcgagcgc gatcaaacga ttggcgttcg
    14881 cggtcaggaa aaggcgtaac tccccgggct atatgacgcg acaagagaac ggcgcgttcc
    14941 ggcagagatc cattgcacca gccggcatcg atgcgccgaa ctatgatgtt cttgttgaat
    15001 cgtctgatgt cgaattcgaa aagacacaag cacctcgagc gcctgatcgt ccagtcctat
    15061 gtgtgggtgc tgcgtgcgcc cagagcaatc gacggccaga aagtcgcatc gctgtcgcga
    15121 cacggcctct atgaggtcag attgctggag ccgtcggaag tggcgcaagg caacgacctg
    15181 ccattttggg tggagctcgt cgacaacaaa cggaatcttt gcctcgacag ctatgcggga
    15241 gacgatatcg aggagaccgc catcgcggtg gaacatctga tttctcgcgc cgaactcctg
    15301 gaccagggct ccgaaagcta acggtgcgca tttgtcggta tgcttcgccg atccggccga
    15361 tcctgccaga tcgcgttgcg ccggcgaccg gcactgcagt cgacatgcga atgcccgaca
    15421 cgcgctgcga ctttgcagca tggaattaga gcgcggggtc taacgcaatt acgctttcgg
    15481 ataatggggc acgcgatcgg ataggcgatt tgcgcgctga cgacccaggg gtggcctgct
    15541 gcggcagctc gtagcagaaa gcggtcccca gccgataagg atgcctggaa atgccgcgcc
    15601 gcgaccggct attcgagcgc gcggttaaat ttgcatttcc tgaacggacg ttgcggtttg
    15661 cgagtatcgc ctatcggctg cgcgtgcgac ctcaaaaacg cggagggacc cagtcgagag
    15721 ccggcacgct cgacaataaa aaatggagtt ggcgatgatg aaggctgcag tccggatttt
    15781 ggcggcggtg ctgctgtact tgctcgcggt tccagcattc gcgcagaaca gccaaccgct
    15841 gcctggtgcc agttcgggcc agggcttgct gagcggaccc caactggagg ccttggtcgc
    15901 tccgatcgcg ctttatcccg atcctttgct atcggagatc atgatggcgt cgacctatcc
    15961 gctcgaggtc gtcgcggcgg accggtgggc caagagcaat gccaaccttc aaggcgatca
    16021 gcttaaagcc gcggtggagc agcagccctg ggaccaaagc gtcaaatcat tggtggcgac
    16081 tgcgagcgtg ctcgacatga tgagcaccaa gctcgactgg acccagcagc tcggcgatgc
    16141 ggtgctggcg cagcagtccg acgtgatgga tgccgttcag cggctccgcc aacgagctca
    16201 gaataccggc aagttgaaat cgaccccgca acagacggtc aacgcgatcc cggcggaggc
    16261 gggagccgcg ccggcgccga gcggagcagc gccgggcgca ggtggagcac cggtggtgca
    16321 gggcggcggg caggtcatag ccattgagcc gaccgatccc gacaccttgt atgtgccgta
    16381 ttacgatccg gccgtcgtct acggcgaatg gccgtattcg gattacccac cctattattt
    16441 cggctatccc ggctatatcg gcgccggcat t
//
LOCUS       DATGEM010000130         4306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_537244, whole genome shotgun sequence.
ACCESSION   DATGEM010000130 DATGEM010000000
VERSION     DATGEM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537244"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VKQ27_04170"
     CDS             complement(<1..241)
                     /locus_tag="VKQ27_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HLY88157.1"
                     /translation="MKLSVALKLGRVSNLPTVWSNVLTGMALAGAATADCRLLILVVS
                     LSLFYIGGMFLNDAFDHGFDAKARPERPIPSGQVRA"
     gene            complement(273..1469)
                     /gene="eboE"
                     /locus_tag="VKQ27_04175"
     CDS             complement(273..1469)
                     /gene="eboE"
                     /locus_tag="VKQ27_04175"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HLY88158.1"
                     /translation="MKIGADGAHLTYCSNIHPGESWADVRANIGRYFPAVRDRVAPGQ
                     MFGIGLRLSARAARELGSGDALAEFRAFLDRNKLYVFTVNGFPYGTFHGTRVKEEVYL
                     PDWRDQERLRYTDELADLLVQLLPDGVDGSISTVPGAFKPAMRNGADVDKIADNMLRH
                     VAKLVEIKRHSGRQIALAIEPEPHCYLETIDESVRFFRGHLFSAAAVRRLGELTDLDP
                     GAAAAAMHDHIGLCLDLCHAAVEFEDPAGCIRDLAEAGIRVTKLQISAGLRLPELTAA
                     AVTALQQFDDPVYLHQVVEEGPNGIRRYADLPEALATATGKADGREWRVHCHVPIFMD
                     DLGAVSSTQSFISEVLGIHRRHPVSGHLEVETYTWNALPEHLRAGGMEQAIARELNWV
                     KQELAA"
     gene            complement(1466..2674)
                     /locus_tag="VKQ27_04180"
     CDS             complement(1466..2674)
                     /locus_tag="VKQ27_04180"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HLY88159.1"
                     /translation="MSVELVKPGAESGTEAVYHQEFSVRYDYPVHFTEHLFARNNPVF
                     VQSIVRREPAKRHRLLVFVDANVAASWPTLVHDIAAYSEGHAESLELVGRPEVVAGGE
                     QVKNDPALVTGLQRHLVDLKIDRHAFVVGIGGGAFLDMIGFVAATTHRGIRHIRVPTT
                     VLGQNDSGVGVKNGINAFGMKNLLGVFAPPFAVLNDFDFLRTLHPRDKVAGIAEAVKV
                     SLIRDGLFFEWLEGNAEALRECAPAAMARMIRRCAELHMRQIAHGGDPFESGSARPLD
                     YGHWSAHKLEGLTGHELRHGEAVAIGLALDARYSVQVGMLAAGAEERVYQLLKHLGFY
                     LWHPAMETRDEQGRLIILRGIEEFREHLGGELSITLLRDIGRGEEVHAMDSNEILRAM
                     AWLRRKETGQ"
     gene            complement(2707..3630)
                     /locus_tag="VKQ27_04185"
     CDS             complement(2707..3630)
                     /locus_tag="VKQ27_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLY88160.1"
                     /translation="MRFIDPHIHMSARTTDDYERMAAAGIVALIEPAFWLGQPRTQVG
                     SYIDYLSSLVGWERFRAAQFGIHHYCTIGLNSKEANNEALAEQVLEILPRYLVKEGVV
                     AVGEIGYDDQTAVEDKYYRAQLALAKEFDMLVQVHTPHRDKKSGTIRSMDVAIEMGVK
                     PERVVIDHNNEETVQDVLDRGFWAAFTIYPNTKMGNERMVEIARKYGSHHIFIDSSAD
                     WGVSDPLAVPKTARLMLDRGIAARDVEAICYGNALKAFGQSGQMKEEHWLNPAPIDQR
                     MLFEGNSVLRGQAPQVGESANAARTDSVVFK"
     gene            complement(3655..>4306)
                     /locus_tag="VKQ27_04190"
     CDS             complement(3655..>4306)
                     /locus_tag="VKQ27_04190"
                     /inference="COORDINATES: protein motif:HMM:NF035938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HLY88161.1"
                     /translation="GWDPSDWSVDQAARIYLLLVSGSDGATFLRRLDQLCNTADVGEL
                     VAFYRGLPLYPDQPRYVLRAAEGIRTNMRAVFEAVAHRNPYAAEQLSEPAWNQMVLKT
                     LFIGSTLHPVVGLDRRANPALARMLCDYAHERWAAGRPISHELWRCVGPYARGEMLRD
                     LERVLEKGTDPERTAAALALASSPDPAATGLLWAYPELSIAVNENKVSWQTLANPV"
BASE COUNT          779 a         1389 c         1335 g          803 t
ORIGIN      
        1 ccgccctgac ctgtcccgac gggatcggcc gctccggccg tgctttagcg tcgaagccat
       61 ggtcgaacgc gtcattgagg aacatgccgc cgatgtagaa cagcgagagc gatacgacga
      121 ggatgaggag ccggcagtcc gccgttgccg cacccgccag tgccatgccg gtaagcacgt
      181 tggaccagac cgtcggcaaa ttggatacgc gcccgagctt cagcgcgacg gacagtttca
      241 tgcggccact aattccaagg acatcttccc gctcacgccg ccaattcctg tttcacccag
      301 ttcagttcgc gagcgatggc ttgctccatg ccgcccgcgc gcagatgttc cggaagcgcg
      361 ttccaggtat aagtttcgac ttcaagatgt cccgagaccg ggtgccggcg atgtatgccc
      421 agcacctcgc tgatgaagga ttgggtcgaa gagacagcgc caaggtcgtc catgaaaatc
      481 ggcacgtggc agtgcacgcg ccattcgcgc ccgtccgcct ttccggtggc cgtcgccagc
      541 gcctctggca gatcggcgta gcggcggatg ccattcggtc cttcttccac cacctggtgc
      601 aggtagaccg gatcgtcgaa ctgctgcagg gcagtcaccg ctgccgcagt caattccggc
      661 aggcgcagac cggcgctgat ctgcagcttg gtgacgcgga tgccggcctc tgccagatcg
      721 cgaatgcaac ctgccgggtc ttcaaactcg accgccgcat ggcacaaatc gaggcacagg
      781 ccgatgtggt cgtgcatcgc ggccgcagcg gcgccgggat ccaggtcggt gagttcgccc
      841 aggcggcgca ccgcggcggc gctgaacagg tgcccgcgga aaaaacgcac gctctcgtcg
      901 atggtctcca ggtagcaatg cggctccggc tcgatcgcca gcgcgatctg gcgcccgctg
      961 tggcgcttga tttccaccag cttcgcgaca tgccgcagca tgttgtcggc gatcttgtcg
     1021 acatcggccc cattgcgcat ggccggcttg aatgcacccg gcacggtgct gatgctgccg
     1081 tcgacgccgt cgggcaagag ctgcaccagc aggtcggcca gttcgtcggt gtaacgcagt
     1141 ctttcttggt cgcgccagtc cggcaggtag acctcctcct taacgcgcgt gccatggaag
     1201 gtaccgtagg gaaaaccgtt gacggtgaag acatagagct tgttgcggtc gaggaatgcg
     1261 cggaactcgg ccagtgcatc gccactaccc agttcgcgcg ccgcgcgcgc ggacaggcgc
     1321 agaccgatac cgaacatttg tccgggcgca acgcgatcgc gcaccgcggg aaaatagcgc
     1381 ccgatgttgg cgcgcacgtc ggcccaggat tcgccgggat ggatgttgct gcagtaggtc
     1441 aggtgagcgc catccgcgcc gatcttcatt gtccagtctc ttttcggcgc agccaggcca
     1501 tcgcgcgcag gatttcgttg ctgtccatgg cgtgcacttc ttcgccacgg ccgatgtcgc
     1561 gcagcagcgt gatggaaagc tcgccaccga gatgctcgcg gaattcctcg attccacgca
     1621 ggatgatcag gcggccctgc tcgtcccgcg tctccatcgc cggatgccac agataaaaac
     1681 caagatgctt gagcaattgg taaacgcgct cttctgcacc ggccgccagc atgccgacct
     1741 gaacggagta acgcgcatcg agtgccaggc cgattgcgac tgcttcgccg tggcgcaatt
     1801 cgtggccggt cagcccttcg agcttgtgcg ccgaccagtg gccgtaatcc agcgggcgtg
     1861 cactgcccga ttcgaacggg tcgccgccat gcgcgatctg gcgcatgtgc agctccgcgc
     1921 agcgccggat catgcgcgcc atggccgcag gcgcgcattc gcgcaaggct tcggcatttc
     1981 cctccagcca ttcgaagaac aatccatccc ggatcagcga aaccttcacc gcttcggcaa
     2041 tgccggcgac cttgtcgcgc gggtgaagtg tgcgcaggaa gtcgaaatcg ttcagaaccg
     2101 cgaacggcgg cgcaaacaca ccgagcagat ttttcatgcc gaatgcgttg atgccgtttt
     2161 tcacgccgac gcccgaatca ttctggccga gcacggtggt gggaacgcga atgtggcgaa
     2221 tgccgcgatg cgtagtggcg gcaacgaagc cgatcatgtc caggaacgcc ccgccgccga
     2281 tgccgaccac gaaggcgtgg cgatcgattt tgaggtcgac cagatggcgt tgcagaccgg
     2341 tcaccagcgc gggatcgttt ttcacttgct cgcctcccgc caccacctcc gggcgaccaa
     2401 ccagttccag cgactcggca tgcccttcgg aataagcggc gatgtcatgc accagggtcg
     2461 gccaactcgc cgcgacgttg gcatccacga agaccaagag gcggtggcgc ttggccggct
     2521 cacgccgcac gattgactgc acgaataccg gattgttgcg cgcgaacagg tgctcggtga
     2581 agtgcaccgg gtagtcgtaa cgcacggaga attcctggtg gtataccgct tccgtgccgg
     2641 attccgcgcc cggcttgacc agttcgacgc tcattgctgc cctctcgttg ccatggacgg
     2701 cgcggcctat ttgaatacga ccgaatcggt gcgcgcggcg ttcgcggact ctcccacctg
     2761 cggcgcctgt ccgcgcagca cggagttgcc ttcgaacagc atgcgctgat cgatgggcgc
     2821 gggattgagc caatgttcct ctttcatctg cccgctctgg ccgaaggcct tgagggcatt
     2881 gccgtagcag atcgcctcga cgtccctcgc agcgatgccg cgatcgagca tcagccgcgc
     2941 ggtcttcggt accgccagcg gatcggagac accccagtcc gcgctcgaat cgatgaagat
     3001 gtggtgggaa ccgtacttgc gcgcgatctc caccatgcgc tcgttgccca tcttggtatt
     3061 gggataaatc gtgaaggccg cccagaaacc ccgatcgagc acgtcctgca cggtttcctc
     3121 gttattgtgg tcgatcacca cgcgctcggg cttcactccc atctcgatcg cgacgtccat
     3181 gctgcggatg gtgccgctct tcttgtcgcg gtgcggcgtg tgcacctgca ccagcatgtc
     3241 gaattccttg gcgagcgcga gctgcgcgcg gtagtacttg tcctcgaccg cggtttggtc
     3301 gtcgtagccg atctccccga ccgcaaccac gccttccttg accaggtagc gcggcaggat
     3361 ttcgagcacc tgctccgcca gcgcttcatt gttggcttcc ttgctgttca ggccgatggt
     3421 gcagtaatga tggatgccga actgcgcggc acggaaacgc tcccagccga ccaggctgga
     3481 cagatagtcg atatagctgc cgacctgggt acgcggctgc ccgagccaga acgccggctc
     3541 gatgagggcg acgatgccgg cagcggccat gcgctcgtag tcgtctgtgg tgcgtgcgct
     3601 catgtggata tgcgggtcga tgaatcgcat gtttgcctcc agtagtcaaa gcggtcagac
     3661 cggattggcc agcgtctgcc agctcacctt gttttcgttt accgcaatgg aaagctcagg
     3721 atacgcccac aataacccgg ttgccgcggg atcgggcgaa ctggccagcg ccagagcggc
     3781 ggcagtgcgt tcgggatcgg tgcccttttc gaggacgcgt tcaaggtcgc gcagcatctc
     3841 gccgcgggca tacggtccga cgcagcgcca caattcatgg ctgatcggcc gtcccgccgc
     3901 ccagcgctcg tgcgcatagt cgcacaacat gcgtgccaac gcgggattgg cacgccggtc
     3961 caggccgacc accggatgca aggtgctgcc gatgaacagc gtcttcagca ccatctggtt
     4021 ccacgccggt tcggacaatt gttcggccgc atagggattg cggtgtgcca ccgcctcgaa
     4081 cacggctctc atgttggtgc gaatgccctc cgcggcacgc aagacataac gcggctggtc
     4141 cggatacaag ggcagcccgc ggtagaaggc aaccagttcg ccaacatcgg cggtattgca
     4201 cagttgatcg aggcggcgca aaaaagttgc gccatccgag ccagagacca gcagcaggta
     4261 aatacgcgcc gcctggtcca cgctccagtc cgacgggtcc cagcct
//
LOCUS       DATGEM010000131        10030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1676829, whole genome shotgun sequence.
ACCESSION   DATGEM010000131 DATGEM010000000
VERSION     DATGEM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676829"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..977)
                     /locus_tag="VKQ27_04195"
     CDS             complement(96..977)
                     /locus_tag="VKQ27_04195"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88162.1"
                     /translation="MRDLPGLHEWAGPRWPDSPAALQIHRQLDNGESPMNRFAGKTAV
                     IAGGARGIGEKVAEDIVAQGGNVVIGDMNRDLGEELSSRLGPANAKFVELDVSRSSDC
                     EVALDLAQSAFGGVDLLVNCAIAMNAGPLIELSEDAWRKTLDVGLTGTFLMSRAFAAR
                     LIREKKPGAIVNISSIGGQHPYGGAGSYSAVKAGITLLTEQMALEWAQYSIRVNALAP
                     GTIETPLTTYLKDPEVRESRRGAVPLGRIGLPADISNGVLYLLSDDASWVTATTLTID
                     GGVSRSLFNHMKGRKWS"
     gene            complement(1075..1824)
                     /locus_tag="VKQ27_04200"
     CDS             complement(1075..1824)
                     /locus_tag="VKQ27_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88163.1"
                     /translation="MAKLQGKIALITGGGSGQGLATAKRFVEEGAFVYIAGRRQAELD
                     NAVSEIGRNVVAVQTDVSNLADLDRLYARIASEKGKIDIVFAAAGIVEAQPLAHMTEE
                     SFDKQFAVNAKGLAFTVSKALPLLNDGASIILVTSIAERKGLAALGVYSATKAAVGSF
                     VRTWTAELSERRIRVNAVCPGPIDTTIFEQLVPNLGMVEKFKAEDAARVALKRLGRPE
                     EIANAVLFLASDESSFVTGVDFAVDGGLTAI"
     gene            complement(1912..2661)
                     /locus_tag="VKQ27_04205"
     CDS             complement(1912..2661)
                     /locus_tag="VKQ27_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88164.1"
                     /translation="MSRLQNKVAVITGGTSGIGLAAAQRFVAEGAFVYIFARRQSELD
                     KTVASIGQNIAGVQGDVRNLDDLDRLYAKIASDGRKLDVVVANVGAVNSVKLEDVTIE
                     TFNHNFDTNARGVLFTVQKALPLLNNGASVILTSTIASLRGFPGRSAYAASKTALRSY
                     ARTWTMELKDRGIRVNTITPGPCDTPLIDAETSSPEEAAAVRTKHAGNIPLGRMARPE
                     EIAAAMLFLASEDSSFVAGSELLVDGGMCSV"
     gene            complement(3183..4274)
                     /locus_tag="VKQ27_04210"
     CDS             complement(3183..4274)
                     /locus_tag="VKQ27_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88165.1"
                     /translation="MANKVLVAGASGLIGAAAIEAFLDAGWNVVGVSRRKPELLSGRK
                     FDFVSVDLRDEKASREALSPLADVTHVAYTAIYENADNLVGGWSDADQIEANNAMLRN
                     VVEPLLSGGAALKHVSILQGTKAYGVHLHPIAVPARESDPRDDHKNFFFDQQDYVRDM
                     GARHGFSWTVLRPQLVTGKIPGALNLLPAIGVYAAIRREKGEPFSFPGGPSFVWEAVD
                     ADLVGKVIAWSAETPQASNEIFNVTNGDVFSWRSVWPAMAETLGVEVAADEPFSIAGY
                     IRDNADVWAKIVAKHGLASGDLRSFVAQGDQHADFAFASGAPAGPVAFVSTVKLRKAG
                     FNAAVDTRDAFQAAFQSFIDRKLLPPARG"
     gene            4858..5565
                     /locus_tag="VKQ27_04215"
     CDS             4858..5565
                     /locus_tag="VKQ27_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HLY88166.1"
                     /translation="MRQNPAAVPPLSRAMLASANFEATVAGETPVQRTLSNEERTRSL
                     EDTMRLRPEGPLWVFAYGSLIWNPVLHYEERRDATLSGWHRAFCLTTRAGRGAPDRPG
                     LVLALDEGGRCDGIAYRLAEEDLALELAMLWRREMLFGSYVPRWVTLKDRSGNGLATA
                     LTFVANRTIDRYEPRIDRATAIHRLATARGSLGSSAQYLFQTRDALLECGILDDELEA
                     LAADVEVYRGRFPDLCN"
     gene            5589..6650
                     /locus_tag="VKQ27_04220"
     CDS             5589..6650
                     /locus_tag="VKQ27_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88167.1"
                     /translation="MAKKVLIVGYSGIIGSATAAAYARKGWDVLGLARNPVALDGVST
                     IGADLLDSGALSATLAGHRPDRLIIAAWLRQESEAKNIAVNGAMVRNILNAVSPQGSL
                     EHVALVTGLKHYLGPFEAYGKGKLPHTPFREDQPRLDIPNFYYAQEDELHAAAMRDGF
                     AWSVQRAHTVIGKAVGNAMNMGSTLAVYATICRETGRPFEFPGSQAQWDGLVDMTDAN
                     LLAEHLLWAADTPGACNRAFNVVNGDVFRWKWMWGQIADWFGIEAVPFDGVVRPLETK
                     LANDSGLWREIAERHGLAEPNIDRLASAWHTDADLGRPLEVVADMSLSRRLGFTAYKP
                     SDDAFFELFKAMRADRLLP"
     gene            complement(6682..7665)
                     /locus_tag="VKQ27_04225"
     CDS             complement(6682..7665)
                     /locus_tag="VKQ27_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY88168.1"
                     /translation="MSFFESIAPPVEELSTYWKLVMLDDLAELRTFARIASAGSLTQA
                     AREMDLALSVVSKRLASLERRTQTRLVNRNTRRMSLTDEGARLLENAQRILTEIDEAE
                     TMLSHGRAEPAGTLRVSAPMGLGRAQVSPVCRRLAAEHPRISIELGLTDRLVSVIDEG
                     IDVAVRIGAQADSDLIMRKLADSYRVIVASPDYLARNGTPRHPDDLIDHECLSYGTVG
                     ANWRLTGPAGERAEMKISSRIRSDNGEVSLDWALAGWGLALKSWVDVEPDIRLGRLVH
                     ILPEWRTDSAPVFALLPSSRLVPSRVRLFLDALGERFSRLRPPPQLAGRPN"
     gene            7701..8807
                     /locus_tag="VKQ27_04230"
     CDS             7701..8807
                     /locus_tag="VKQ27_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HLY88169.1"
                     /translation="MKPILFSPITLGNLTLPSRIVMAPLTRARSAQPGDIPTEMAARY
                     YAQRASAALIISEASYISRQAKGYDGTPGCFTPEQLAGWKKVVDAVHAKGGRIFLQIW
                     HVGRMSHPDFHDGHPPVAPSAMPVDENIWKVDPDTGIGSFVPVRPPRAMTHGEIKQTV
                     QDFRLAARNAVDIGFDGVEIHGANGYLVDQFMRTTSNVRTDEYGGSRENRLRFVREVI
                     TAVAEEVGPDRVGIRLAPFLTFRGMGCPDILPTIAEAASFANDMGIAYLHLVEADFDH
                     APEFTEEFRKEMRERFKGNIIMAGLYDREKADWVLEKGYADMVAFGRLFLANPDLPYR
                     LEHELPMNPVDNAHLMGGGDEGYTDYPFFARDAA"
     gene            complement(9011..9469)
                     /locus_tag="VKQ27_04235"
     CDS             complement(9011..9469)
                     /locus_tag="VKQ27_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY88170.1"
                     /translation="MIDERLAALKVELPPFAEGLGDYAPWTRVGSLVSTSGQFPWLNG
                     ELAYVGRIGETISRAEGYAAARLCALNAIAQLRNACGGDLGRVRSVVRVEGQLFCTPG
                     HVEPAGVLDGASHLINDVFRERGRHSRAVTGVASMPLGSSVLLYVHAEIA"
     gene            9773..>10030
                     /locus_tag="VKQ27_04240"
     CDS             9773..>10030
                     /locus_tag="VKQ27_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY88171.1"
                     /translation="MTKTSSGITIYSDKISGSTRRVTTVLEHLDIEYAFEHLDIFKGE
                     QMEPSFLAMNPNHFVPVMTDGDLTLWDAASINIYISDTYDSS"
BASE COUNT         1924 a         3182 c         3124 g         1800 t
ORIGIN      
        1 ggccggtcgg agtagggcgc ctgcaatcga gcgataatcc aggatcccgg agttaacccg
       61 atcgatcagg caccctcccc ctcaacggcg ggccgtcagc tccatttgcg gcccttcatg
      121 tggttgaaga gggatctgct cactccccca tcgatcgtca gcgtcgtggc cgtgacccac
      181 gaggcgtcgt cgcttagcaa gtacagcacg ccattggaga tatcggccgg caggccgatg
      241 cgaccgagcg gcacggcccc tctgcgggat tccctgacct cggggtcctt caggtaggtc
      301 gtgagcggcg tctcaatcgt tccgggagcg agcgcgttga cgcggatgct gtactgcgcc
      361 cactcgagcg ccatctgctc ggttagcagc gtgatgccag ccttgaccgc actgtacgag
      421 ccggcgccgc cataaggatg ctggccgcca atcgacgaga tgttcacgat cgcgccgggc
      481 ttcttctcgc gtataaggcg ggcggcaaaa gcccgggaca tcaggaatgt ccccgtaaga
      541 ccaacgtcga gcgtctttcg ccacgcgtcc tcggacagtt cgatcagcgg gcccgcgttc
      601 atcgcgatcg cgcagtttac gagcagatca acgccgccga aggcggattg agcgagatca
      661 agcgcgacct cgcagtccga gctgcgactt acatcgagtt cgacgaattt ggcgtttgcg
      721 gggcccaatc tcgaggacag ctcctcgcca aggtcacggt tcatgtcgcc aatgacgacg
      781 tttccaccct gcgcgacgat gtcctcggcg accttttcgc caatgccgcg cgctcctccc
      841 gcgatcaccg cggtcttgcc ggcaaaccta ttcatcgggc tctctccatt atcaagctgg
      901 cggtgaattt gcagcgcggc agggctgtcc ggccagcgtg gtccagccca ctcatgaagc
      961 ccaggcaggt ctctcaagag cctgcgggtc caactccgtt gtcgctgacg gcctcgcaca
     1021 cccctgtccg agcttcgtga tccgaaaacc cggctctggc agcggccgcc ggcctcagat
     1081 cgccgtcagg ccgccgtcga cagcgaaatc gacaccggtg acgaagctgg attcgtcgga
     1141 ggcgaggaag aggacggcgt tcgcgatctc ttccgggcgg ccgagccgct tgagcgcgac
     1201 cctcgcggcg tcctcggcct tgaacttctc aaccatgccc aggttcggca ccagctgctc
     1261 gaagatcgtc gtgtcgatgg gtccggggca aacggcatta acgcggatac gacgttccga
     1321 cagttcggcc gtccaggtcc gtacgaagga gccgactgcc gccttggtgg cgctgtagac
     1381 gccgagcgca gcgagaccct tccgttcggc gatggaggtg acgaggatga tcgacgcgcc
     1441 gtcgttcaga agcggaaggg ccttgctgac agtgaaggcc agtcccttcg cattgactgc
     1501 aaattgcttg tcgaagcttt cctcggtcat atgggcaagc ggttgagctt cgacgatgcc
     1561 ggcagcagca aagacgatgt cgatcttgcc cttctcgcta gcgatcctgg cgtaaagccg
     1621 atcgaggtcg gcgaggttcg aaacgtcagt ttgcaccgcg acgacgttgc ggccgatctc
     1681 cgacactgca ttgtcgagtt ccgcctgccg gcgcccggcg atgtagacga atgcaccttc
     1741 ctcgacgaaa cgcttggcgg tggcgagacc ctgtccgctg ccgccaccag taatgagcgc
     1801 gatttttcct tggagctttg ccattggaat cctccttatg tgtgggaccg gcctgtcggg
     1861 ggatctaaag cgcaggcctg gatcgcacgg ataatgattg tccggactgg ctcagaccga
     1921 gcacataccg ccgtcgacga gcagctcgct gccggcaacg aagctgctgt cctcggacgc
     1981 cagaaacagc atcgcggccg cgatttcctc ggggcgtgcc atgcggccaa gcgggatatt
     2041 cccggcatgt ttggtacgaa cggctgcggc ctcctcgggt gagctcgtct cggcatcgat
     2101 cagcggcgtg tcgcaggggc cgggcgtgat cgtgttgacc cggatgccgc gatccttgag
     2161 ctccattgtc caggtccggg cataggagcg cagggccgtc ttggaggccg cataggcgct
     2221 ccgacccggg aagccacgca gggacgcgat cgtgctggtg aggatgacgg acgcgccgtt
     2281 gttcaggagc gggagcgcct tttgtaccgt gaacagcacg ccacgggcgt tggtatcgaa
     2341 attgtgattg aaggtctcga tcgtgacatc ctcgagcttc acgctgttca cggcgccgac
     2401 attggcgacc accacatcga gcttgcggcc gtccgaggcg atcttcgcat agagccgatc
     2461 gaggtcatca agattgcgga cgtcgccctg cacgcccgcg atgttctggc caatcgaggc
     2521 gaccgtcttg tccaactcac tctggcggcg ggcaaaaata taaacaaatg cgccttccgc
     2581 gacaaaccgc tgggccgccg ccaatccaat cccgctcgtg ccgccggtaa tcacggcgac
     2641 cttgttctgc aatctcgaca tcatcgcttc cttgatcgca agagccgggt tttacgctcg
     2701 ccgtctctga gccaaggggt acgcctctgt ccggaaggga agtaggcgag atgcttgaat
     2761 attctttatt cccatttgga atacgcgaca ttccgcagcg tgggtcgggg cgagtctggc
     2821 ctcgcagcgg caccatatag gaagcaatcg acgcgaaagg ctgacgtact tgcctgcggt
     2881 accgacatcg ctcgaacgca tgacaagccg gcttggagat cgccgccaca tagtctgccg
     2941 ccgcgcgatc atgtccggcc ggcgaaacgg cgaaaacacg ctcaaggaga cgatttcggt
     3001 tgaacggcgc agagaaaacc gcgtggatca caagtggcaa gccgttcgcg tcgcgtatgc
     3061 aggacgtcga accgatcggt cgagacgacg ccctgcatgc ccggcgttca agaagaggcg
     3121 ctcagtctga gccaggtgag tggcgagccg ggtcagccgg ccacccgcct gtcgctcaca
     3181 tcttagcccc gggccggcgg aaggagctta cggtcgatga acgactggaa ggcagcctgg
     3241 aaggcatcgc gggtgtcgac cgccgcgttg aaaccggcct tccggagctt gaccgtgctg
     3301 acgaacgcca ccgggcccgc gggggcaccg gacgcgaacg caaagtcagc atgctgatca
     3361 ccctgcgcga cgaacgagcg cagatcgccg ctcgcaaggc catgcttggc gacgatcttc
     3421 gcccaaacgt cggcattgtc gcgaatgtac ccggcgatgc tgaagggctc gtcggctgcc
     3481 acttccacgc ccagcgtctc agccatcgcc ggccacacgc tgcgccagga gaagacgtcg
     3541 ccgttggtga cgttgaagat ctcgttcgac gcttgcggcg tttccgccga ccaggcaatg
     3601 accttgccca cgagatcggc atcgaccgct tcccagacga aggacgggcc gccggggaag
     3661 ctgaagggtt cgcccttctc gcgacgaatt gcggcgtaga cgccaatggc gggaagaagg
     3721 ttaagagcgc ccggaatctt gcccgtcacc agctggggac gcagcacggt ccacgaaaaa
     3781 ccatgccgcg cgcccatgtc gcgaacatag tcctgctgat cgaagaagaa gttcttgtga
     3841 tcgtcgcgcg ggtcgctctc gcgagccggg acggcgatgg gatgaaggtg aacaccgtag
     3901 gccttcgttc cctgcaggat ggaaacgtgc ttgagagcag ccccgcccga gaggaggggc
     3961 tcgaccacat tgcgcaacat cgcgttattg gcctcgatct gatccgcgtc cgaccagccg
     4021 ccgacgaggt tgtcggcatt ctcgtagatc gccgtataag cgacgtgcgt cacgtcggcg
     4081 agcggggaca gcgcctcgcg cgaagccttc tcgtcgcgca ggtccaccga gacgaagtcg
     4141 aacttccgcc cactcagaag ctccggcttg cgccgcgaaa cgccgacgac gttccagccc
     4201 gcgtccagaa aagcttcaat tgcagcagcc ccgatgaggc cgctcgcgcc ggctaccaaa
     4261 accttatttg ccatgtctct ctctttcctc tttgcaatca ggcaccattc cgaaaaggat
     4321 catcacaacc aaaggacgcc gatcgtcgct cgcggccatt gggccgaagc attcctggcg
     4381 aattcccatg cgcttggtcg gttttcacct cccatcgccg cgcgaacggt gtcgcgtgac
     4441 gaaaggcatg gatcttcggc ttcatcacct tctactcggg ccagagcagc tcgacgcttg
     4501 ccctctcgct atatcaatca tccccgagca aatccggtag atgtcattcg gtgatattct
     4561 tcatgctcaa acggaataac ggcatgccgg ctcgcgccga tcgtcgggag cgagcatgtc
     4621 gtcaccccgg ggagatcccc gtgcgcggcg atatatccct gtaatcacgg acatctagcg
     4681 gagtcgggag accgtcggga ggcgaccact tcgccgatca cggggatgca aacccctcga
     4741 cggatcgatt atctccgtct gttcaagagt ctctcgcctg agcggttgtt tttcgcaggc
     4801 gtcgccgcgt caatacttac gggcggacgg gctacttcgc ccatggagac cgaatagatg
     4861 aggcagaatc cggcagcggt tccgccgttg tccagggcaa tgctggcatc ggcgaatttc
     4921 gaagcgacgg tggcaggcga aacgcccgtc cagcgaacgc tgagcaatga ggagcggacc
     4981 cggtccctcg aggacacgat gcggctacgg cccgaggggc cgctgtgggt cttcgcctac
     5041 ggctcactga tctggaaccc cgtgcttcac tatgaggagc ggcgcgacgc aacgctctcc
     5101 ggttggcatc gagccttctg cctgacaacg cgagccggtc ggggtgcgcc agaccgtccc
     5161 gggctggtgc tggcactgga cgagggaggc cggtgcgacg gcatcgccta ccgcctggcg
     5221 gaagaggatt tggcgctgga gctggcgatg ctgtggcggc gcgaaatgct gttcggatcc
     5281 tatgttccgc gctgggtgac cctcaaggat cggtcgggca atgggctcgc gactgccctc
     5341 accttcgtcg caaatcgaac gatcgaccgg tacgaaccgc gcatcgatcg agcgactgca
     5401 atccaccgcc ttgcaacggc gcgaggcagc ctgggcagca gcgctcaata tctgttccag
     5461 acacgcgatg cactgctgga atgcggaatc ctggatgacg agctcgaggc gcttgctgcc
     5521 gacgtcgaag tgtatcgcgg acggtttccc gacctctgca attagaccct ggaacaagga
     5581 gacgaacgtt ggcgaagaag gttctgatcg tcggatatag cggcatcatc ggtagtgcga
     5641 cggcagccgc ctacgcgcgc aagggttggg acgtgttggg cctggcgcgc aacccggtcg
     5701 ccctcgatgg cgtcagcacg attggggcag accttctgga ttcgggggcg ctttcggcaa
     5761 ccctcgcggg acatcggccc gatcggctca tcatcgcggc ctggcttcgc caggagagcg
     5821 aggcaaaaaa cattgccgtc aacggcgcca tggtccgcaa cattctcaat gcggtgagcc
     5881 cgcaggggtc gctggagcat gtggcgctgg tgaccggcct caaacattat ctcggaccat
     5941 tcgaggcgta cgggaaaggt aagcttcccc atacgccctt ccgcgaggac cagccacggc
     6001 tcgacatccc caacttctat tatgcgcagg aagacgaact ccacgccgcg gccatgcgcg
     6061 atggcttcgc ttggagcgtc caacgcgctc acactgtgat cggcaaggcg gtgggcaacg
     6121 ccatgaacat gggctcgaca ctcgcggttt acgcgacgat ctgccgcgaa accggccggc
     6181 ctttcgagtt tcccggatcg caagctcagt gggacgggct ggtcgacatg accgacgcca
     6241 atctcctcgc cgagcatctt ctgtgggcgg cggacacgcc gggagcatgc aaccgagcat
     6301 tcaatgtcgt gaacggcgac gtcttccgct ggaaatggat gtgggggcaa atcgctgatt
     6361 ggttcgggat cgaagcggtg ccgttcgatg gcgttgtgcg gccgctcgag accaagctcg
     6421 caaacgattc gggcctgtgg cgcgaaatcg cggaacgcca cgggctcgcg gaaccgaaca
     6481 tcgatcgcct cgcatcggca tggcacaccg acgccgatct cggccgcccg ctcgaagtcg
     6541 tcgccgatat gtcgctgagc cggcggctgg gctttaccgc ctacaagcca agcgacgatg
     6601 ccttcttcga attgttcaag gcgatgcgcg cggatcgcct tcttccctga caccatcgcc
     6661 ggcccaatcg tgaggggatg ctcaattggg ccggccggca agctggggcg gcggacgaag
     6721 gcgggagaag cgctctccga gcgcgtcgag aaaaaggcgc acccgggacg ggacgagacg
     6781 gctgctcggc aagagcgcaa agaccggcgc agaatccgtg cgccactcgg gcaaaatgtg
     6841 caccagcctg ccaagccgaa tatcgggctc gacatccacc caggacttga gcgccagtcc
     6901 ccagcccgcc aacgcccaat cgagagagac ctcgccgttg tcgctgcgga tccgcgacga
     6961 gatcttcatc tcggcgcgct cgcctgccgg cccggtgagc cgccagttcg cgccgacggt
     7021 gccgtaactc aagcactcgt ggtcgatcag atcgtccggg tggcggggcg taccgttacg
     7081 cgcgagatag tcgggcgacg cgacgatgac ccggtagctg tcggcgagct tgcgcatgat
     7141 gaggtcggaa tccgcttggg cgccgatgcg aacggcgacg tcgattcctt cgtcgatcac
     7201 cgacacgagg cggtcggtga ggccaagctc gatcgagatc ctcgggtgct ctgcggcaag
     7261 ccgccggcaa accgggctca cctgcgccct gcccaagccc atcggcgcgc tgaccctaag
     7321 ggttcccgcc ggctcggctc ggccgtgact tagcatcgtc tcggcctcgt caatttcggt
     7381 gaggatgcgc tgcgcgtttt cgagcagccg cgcgccctca tcggtcagcg acatgcggcg
     7441 ggtgtttcgg ttcaccaggc gggtctgggt gcgccgctcg agcgatgcca gcctcttgct
     7501 gacgacgctg agcgccaggt ccatttctcg cgccgcctgc gtaaggctgc ctgcactggc
     7561 aatgcgggcg aacgtcctca attccgcaag atcatctagc atgacgagtt tccagtaagt
     7621 ggagagttct tccactggtg gagcgatact ctcgaagaac gacaaggatt agatcgggcg
     7681 ccaggacaag gagcgccgtg atgaaaccaa ttctcttttc tccgatcacg ctgggaaatc
     7741 tgacgcttcc atcgcgcatc gtgatggcgc cgctgactcg cgcgcgcagc gcgcagcctg
     7801 gcgacatacc caccgaaatg gccgctcgct attatgcgca gcgggcctcg gcggcgctca
     7861 tcatctcgga ggcttcctat atttcgcgcc aggccaaggg atatgacggc accccgggct
     7921 gcttcacgcc cgagcaactt gcgggctgga agaaggtcgt cgacgcggtg cacgcaaaag
     7981 gcggcaggat cttcctgcag atctggcacg tcggccgaat gtctcatccc gacttccacg
     8041 acggccatcc accggtcgct ccctcggcga tgccggtcga cgagaatatc tggaaggtgg
     8101 accccgatac gggcattggc agcttcgtgc ccgtgcggcc gccccgcgcg atgacgcatg
     8161 gcgagatcaa gcaaaccgtc caggacttca ggctggcggc gcgcaacgcc gtcgacatcg
     8221 ggtttgacgg cgtcgagatc catggcgcaa acggctacct tgttgaccag ttcatgcgca
     8281 cgacctcgaa tgtgcgcacg gacgaatatg gcgggtcgcg ggagaaccgc ctgcgctttg
     8341 tgcgcgaggt catcaccgca gtcgccgagg aggtcggccc agaccgggtc ggaatccgcc
     8401 tggcgccgtt cctgaccttt cgcgggatgg gctgtcccga catcctgccg acgatcgccg
     8461 aagcggccag cttcgcgaac gacatgggga tcgcctatct ccacctcgtc gaggccgatt
     8521 tcgaccatgc gcccgagttc acagaggagt tccgcaagga gatgcgcgag cgcttcaagg
     8581 ggaacatcat catggcgggg ctctacgatc gcgagaaggc cgactgggtg ctggaaaagg
     8641 gctacgccga catggtcgct ttcggccgac tcttcctggc caatccggat cttccctatc
     8701 ggctcgagca tgagctgccg atgaatccgg tcgacaatgc ccatctcatg ggtggcggcg
     8761 acgagggcta taccgactat cctttcttcg cgcgcgacgc ggcctgagcc gcttcgcccc
     8821 gggcagacgc ggcgcgggct tacgaacggg cgatgatcgt ggacgagtcc gttctcgcca
     8881 gcgccgtggc cgaagggatc aatcgcgaca cgatgcgccg tcgcgcgtgc cggggaatga
     8941 acggcgcccg ggcggccccg aatggtcgga ccatgtctgc agaaaggcgc gccgtgcagc
     9001 atcggtcgcc tcatgcgatt tcggcgtgaa cgtagagaag gaccgaagat ccaaggggca
     9061 tgctcgcaac accggtcacg gcacggctgt ggcgtccgcg ttccctgaaa acgtcgttga
     9121 tcaggtgcga agcgccatca aggacgccgg caggctcgac atggcccggc gtgcaaaaca
     9181 actgtccctc gacacgcacg acgctgcgca cacggcccaa gtcaccgccg cacgcattcc
     9241 tgagctgtgc aatggcgttg agcgcacaga gtctggctgc ggcatagccc tccgcccggg
     9301 agatcgtctc gccgatcctg ccgacatagg cgagttcgcc gttcagccag ggaaactgcc
     9361 cggaagtcga aaccagcgag cctaccctcg tccaaggcgc gtaatccccc aacccttcgg
     9421 caaatggcgg cagctcgacc ttcagcgcgg caagccgttc gtcgatcatg acccactccc
     9481 tggcaatcga agttgcccaa atctggcgat acagcaggtt tcatagcaca atttatcgct
     9541 tgataacagc caccgccgct ccgcgctcac gcagccgggg cccagcaacc aattgctatt
     9601 ggagaatttt ttggagctcc gcacgctgcc ggagctcatt attccaagct cgaataatca
     9661 ttagtcgaat agatagacta ccccgatgcc gtcggcgcgg caattgcaag caactgcacc
     9721 cggcgactgc cggccaaagc cataacaccc ctgcacagca aggagaatat ccgtgaccaa
     9781 gacctcaagt ggaatcacga tctacagcga caagatttcg ggaagcaccc ggcgggtgac
     9841 gacggtactc gaacacctcg acatcgagta cgcgttcgag catctcgata tcttcaaggg
     9901 cgagcagatg gagccctcgt ttctcgccat gaaccctaac cacttcgtcc cggtgatgac
     9961 ggacggggac ctcacgctgt gggacgcggc ttccatcaac atctacatct ccgacactta
    10021 tgacagctcg
//
LOCUS       DATGEM010000132         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1676431, whole genome shotgun sequence.
ACCESSION   DATGEM010000132 DATGEM010000000
VERSION     DATGEM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676431"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VKQ27_04245"
     CDS             <1..243
                     /locus_tag="VKQ27_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88172.1"
                     /translation="FAAIGVVLAFVVAAAINHAGLTWTPPGNANAVPFRLAIPSSRPL
                     LLAGTWIGLVLVATIAALLPANRAARLPVVDALRHV"
     gene            251..1012
                     /locus_tag="VKQ27_04250"
     CDS             251..1012
                     /locus_tag="VKQ27_04250"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HLY88173.1"
                     /translation="MRFLLLMLALTLGAPAFAQTPRDIVAAADNVRNPGQPFHSVDTL
                     TEYVSGVARNKDQLVVFSKQDPGTRQYRNLVRYLAPPRDAGKMVLLDSHNLWFYDPAS
                     KASVRISPQQRLIGQAAVGDILTTNFAIDYAATLLGDETISDAEHQQRACWHLDLKAV
                     TDTATYNRIEYWVEHGSYRPIKARFFSDSGRLLKILYYRGYQQRLGGVRPSEAIIIDA
                     VDSSLVTSLQFGDLQAQDIPEAWFQRDYLPRVPAE"
     gene            1016..>2092
                     /locus_tag="VKQ27_04255"
     CDS             1016..>2092
                     /locus_tag="VKQ27_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88174.1"
                     /translation="MRVASLWWGVALGCLLAAPAFGAGQDNDLDLIPPAAQAAPSPAA
                     AQAPSSNGAQRNYLEGLFSATPLRDSLAVASPPPTPASWQGRLFLDMRDAWKVTDTLT
                     LNYSGRFNLRAANDLPFPSHENVLNELRETYASWEPAPGTWIDLGRINVKSGVAVGYN
                     PTDFFRSRAVVEPTTADPSVLREDRLGTLMLTGQHTWQGGSITVGFAPRVTLPTAIYK
                     SNDLPSFDPMLDRTNAEFRYFIKGSFSLADDFAPEVLLYHAGNRTKVGTNLTMSMGRQ
                     VVGYLEWAGGVDTSLIDSALRYGRQTGTLPANAPSPIPVDTSGQFQNDLAIGASYASE
                     SNITLNLEYHFYQPGFSRQDWNNWF"
BASE COUNT          369 a          636 c          686 g          401 t
ORIGIN      
        1 ttcgccgcga tcggggtggt gctggcattc gtggtcgcag cggcgatcaa tcatgctggg
       61 ctgacctgga cgccgccagg caatgccaat gccgtgccgt tccggctcgc gatacccagc
      121 agccggccgc tgctgctggc cggcacgtgg atcgggctcg tgctggtcgc gacgatcgcc
      181 gcgttgcttc ccgccaatcg cgccgcgcgc ctgccggttg tggacgcgct gcgtcatgtg
      241 tgaggtcatc atgcgattct tgctgcttat gcttgcgctg acgttgggcg cgccggcttt
      301 tgctcaaacg cctcgcgaca tcgtcgctgc ggcggacaat gtgaggaacc cggggcagcc
      361 gttccactct gtagatacgc tcaccgaata cgtgtccggt gtggcgcgga ataaggatca
      421 actcgtggtg ttctccaagc aggatccggg gacgcggcaa taccgcaatc tcgtccgcta
      481 tctcgcgccg ccgcgcgacg cgggcaagat ggtgctgctg gatagccata acctgtggtt
      541 ctacgacccc gcgtcgaagg caagcgtgcg catttcgccg cagcagcggc tgatcgggca
      601 ggcggctgtc ggcgatatcc tgacgacgaa tttcgcgatc gattacgccg ccacattgtt
      661 gggcgacgag acgatcagcg acgctgaaca tcagcagcgc gcgtgctggc atcttgacct
      721 gaaggcggtg acggatactg cgacgtacaa ccgcatcgag tattgggtgg aacacggcag
      781 ttatcgtccg atcaaggcac gattcttttc agacagcgga cggctgctga agatcctgta
      841 ctaccgcggc tatcagcagc gcctgggtgg agtgcggccg agtgaggcga tcatcatcga
      901 tgccgtggac agctcgctgg tgacctcgct gcaattcggc gatcttcagg cgcaggacat
      961 cccggaggca tggttccagc gcgactacct gccgcgggta ccggcggagt gatgcatgcg
     1021 cgtcgcgtcg ctctggtggg gcgtggcgtt ggggtgcctg cttgcagcgc ccgcgtttgg
     1081 cgccggtcag gacaatgacc tcgatctgat tccgccggcg gcgcaagcgg cgccttctcc
     1141 ggcggcggca caagcacctt catccaatgg ggcacaacgc aactacctcg aaggtttgtt
     1201 ctcagcgaca ccgctgcgag acagccttgc cgtggctagc cccccgccga cgcccgccag
     1261 ttggcagggg cggctgttcc tcgacatgcg cgatgcgtgg aaggtgaccg acacgctgac
     1321 gctgaactac agcgggcggt tcaatctgcg cgcggcaaac gacctgccgt tccccagtca
     1381 tgagaacgtg ctgaacgagc tgcgcgagac ctatgcgagc tgggaaccgg caccgggaac
     1441 gtggatcgat ctcgggcgga tcaacgtgaa gagcggtgtc gcggtcggtt ataatccaac
     1501 agatttcttc cgcagccgcg cggtggtgga gcccacgacg gcggatccgt cggtgctgcg
     1561 cgaggatcgg ttgggcacct tgatgctgac ggggcagcac acatggcagg gcgggtcgat
     1621 cacggttggc ttcgcgcccc gtgtcacgct gccgacggcg atctacaaga gcaacgacct
     1681 gccttcattc gatccgatgc tcgatcgcac gaatgccgaa ttcaggtatt tcatcaaagg
     1741 aagcttctcg ctggccgatg acttcgcgcc ggaggtgctg ctgtatcatg ctggcaatcg
     1801 cacgaaggtc ggcaccaatc tgacgatgtc gatggggcgc caggtcgtgg gttacctgga
     1861 atgggctggc ggcgttgata ccagcctgat cgacagcgcg ctgcgctacg gcaggcagac
     1921 cgggacgctg ccggccaatg cgccatcgcc gattcccgtc gatacctcgg ggcagttcca
     1981 gaatgatctg gcgatcggtg cttcctacgc cagcgagtcg aacattacgc tcaacctgga
     2041 ataccacttc taccaaccgg ggttttcgcg ccaggactgg aacaactggt tc
//
LOCUS       DATGEM010000133         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1019014, whole genome shotgun sequence.
ACCESSION   DATGEM010000133 DATGEM010000000
VERSION     DATGEM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019014"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VKQ27_04260"
     CDS             complement(<1..1661)
                     /locus_tag="VKQ27_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY88175.1"
                     /translation="MAWRIGIDIGGTFTDVALVEEQTGQIAIAKLLTTPHDFGLAVID
                     GIRQGLDTNRIAAADVSLLSHATTVVTNALLENKGARAGFIATRGMRDLLELRRSSRA
                     DLYDLLQDAPAVLVPRRWRFEITERIDAQGEVVTPLAEDELPTIIEAIRAAELQTVAV
                     SFLFSFLNDAHERRVGELLRAALPNVAVYLSSEVLPEIREFERASTTAVCAVVGPVLA
                     SYLDRLQSAITQLGLPKLHVMGSSGGVFDIAEGLRMPAMAVESGPAAGVIAASLAGRQ
                     LGRPNLISFDMGGTTAKASVIAGGEVSVTAEYEVGGSGHANRWMHGTGHPIRVPVIDL
                     AEVSAGGGSIAWVDPGGSLKVGPHSAGAAPGPAAYGRGGTLPTVTDADMVLGYLDREA
                     LLGGDLAIDLAAAERAIESAIAEPLGLTVPEAAARIIEVVNSNMAQALRIVSIERGHD
                     PQEFSLIAFGGAGPVHAVSLAEELQIPEVIVPPAPGAFSALGLVASDLKRDYSRTLYA
                     DVGSIDPERVAEVIASMEQAGREMLAAARVPPERRALLRMADVRY"
     gene            1700..2929
                     /locus_tag="VKQ27_04265"
     CDS             1700..2929
                     /locus_tag="VKQ27_04265"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88176.1"
                     /translation="MRDPRRDGTSRLMLFFAVVYTVEGFCQAKIGVIWQPLTHYLKES
                     QGWDAVHIATSLAVLDVPWVVKPLYGIVSDFFPLFGYRRRSYLLLANAVAVAVFLWAT
                     QTLAPSILIFALALSAIALAIASTVCGALLVENGQRTHESAAFINQQWLWFNVAVMAA
                     SIIGGELIEYLSPAGALHMALGIAAAVPVAALISINLVHEAHAGLNIAEFRHALRGLL
                     DTFRSRTLWLVAGFLFLYYFSPGFGTPLYFYQTDRLHFSQGFIGVLASVSAVGWVAGG
                     LIYRFWLAGMTTRALLVLSIIGGTLGTLTFLGMIDEPTAVAVYFLYGVASMVANIATL
                     SLAAEHCPRGSEGFTFAALMSVINLATPLADTIGAFLYQHAFNAQLAPLIVVSAVATA
                     LILVLLPLLERATAPAR"
     gene            complement(3470..4789)
                     /locus_tag="VKQ27_04270"
     CDS             complement(3470..4789)
                     /locus_tag="VKQ27_04270"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLY88177.1"
                     /translation="MQAEREVVRFLSAYQTIWHAHFPSLFKRAQWHIVTHLCTTGREG
                     AAVGELYGLVKQVFLLDDSTVKERILEIAELKLCVVDPPGGSLSARSVIVPTPALLDL
                     FDRHLVALAKELATSAAALDPTVRLAPPRRLDAAQRGVILQSLVRCREPWRAALERIF
                     DASNLSRARRVEAIRHLNSMSHWTLLHMAVELRYGSSTLAGSEQGILADQMAATLLNL
                     TGQNFQTTRDHISYLTGLGLLERQPGKSLRVALADDAAREFSQALATAAAALPDVARS
                     LGVTEEEPESPFAEDPSVEATLNLRSGRLRPVPPESLHHLVILQPESAVRRVALPPGA
                     LTIGRAPPSALLLEGPEVSRAHCRIDVDGNEVSITDLNSTNGTFVDNKRLSGSAPLPH
                     GALLRIGNYVMTCEYQSEASREDADSTQRRPGFAEITVLRPRRGRTA"
     gene            complement(4881..>6231)
                     /locus_tag="VKQ27_04275"
     CDS             complement(4881..>6231)
                     /locus_tag="VKQ27_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY88178.1"
                     /translation="MPDISIVTPIVIDGAVRAFAANVGHHSDVGGPVPGSISGRARSV
                     FEEGLRLPVIRLVRAGELDEDLLGLIAHNSRDPVERSLDLKVQVAANTRGVRLMRELV
                     ERMGLTAVEEAVDDLLAYTARRLRNRIRDIADGEGSFTAWLDDDGLGGDPVPIRARVV
                     ISGEAMTVDFSGTGPETRGALNVPESALRATVYYAVKTLLDPNLLPNSGMFDCIDIKA
                     ESGTLVAPRFPAAVGARSITCNKIARALFGAFAGLLPVDRVMASSHDSVPAIIFSGPR
                     RVGDDTFVYLETLGGGMGARSDADGMDAIHVHLTNTSNLPAEALEHEYALLVDEYALV
                     PDSGGAGRYRGGLGIARQIRAMRDGIVFSVRSDGHVLGAPGLAGGGDGATAHLIRNGE
                     EELSSKTASLVLGAGETIRIETGGGGGFGDPGERDPAALTADLQDGKISATRAPGGK"
BASE COUNT         1126 a         2241 c         1792 g         1072 t
ORIGIN      
        1 ctgtaccgga catccgccat ccgcagcagc gcccgccgct ccggcggcac gcgtgccgca
       61 gccagcatct cgcgccccgc ctgctccatc gaagcaatca cctcggcgac ccgctccgga
      121 tcgatcgaac cgacatcagc ataaagcgtg cgcgaataat cccgcttcag atcactcgcc
      181 accagtccca gcgcggaaaa cgccccgggt gccggcggca cgatcacctc cggtatctgc
      241 aattcctccg ccagcgacac ggcatgcacc ggccccgccc cgccgaacgc gatcaaactg
      301 aactcctgcg gatcatgccc ccgttcgatc gagacgatcc gcagcgcctg cgccatgttg
      361 ctgttcacca cctcgatgat gcgcgccgcc gcctccggca ccgtcagccc cagcggctcc
      421 gcgatcgcgc tttcgatggc tcgctccgcc gcagcgagat cgatagccag atcgccaccc
      481 agcagcgcct cgcggtcgag ataccccagc accatatccg catccgtcac cgtcggcagc
      541 gtgccgccac gtccatacgc cgcaggcccc ggcgctgcac cggcactatg cggcccaacc
      601 ttcagcgaac cacccggatc cacccacgcg atcgatccac cgcccgcgct cacctccgcc
      661 agatcgatca ccggcacgcg gatcggatgg ccggtgccgt gcatccagcg attggcgtgc
      721 cccgacccgc caacctcgta ttcggctgtc accgacactt cgccaccggc aatcacgctg
      781 gccttcgctg tcgtcccacc catatcgaac gaaatcagat tgggccggcc caactgccgt
      841 cccgccagcg atgccgcgat cacccccgcg gccggcccgg actccacggc catcgccggc
      901 atccgcagcc cctcggcgat atcgaacacg ccgccggacg accccatcac atgcagcttc
      961 ggcaacccaa gctgggtaat cgcgctctgc agccgatcga gatacgacgc cagcaccggt
     1021 cccaccacgg cacacaccgc cgtcgtgctc gcacgttcga attcgcggat ctccggcagc
     1081 acctcggacg acagatacac cgccacattc ggcaatgcgg cccgcagcaa ctcccccacg
     1141 cgccgctcat gcgcatcatt caggaacgag aacagaaacg acacggcgac cgtctgcagt
     1201 tccgccgcgc ggatcgcctc gatgattgtc ggcaattcgt cctcggccaa cggcgtaacc
     1261 acctcgccct gcgcatcgat tcgctcggtg atctcgaagc gccagcgccg gggcaccaga
     1321 accgccggcg catcctgcag caggtcgtac agatcggcgc gcgaggaccg ccgcagctcc
     1381 agcagatcac gcatgccccg cgtcgcgatg aatccggcac gcgccccctt gttctccagc
     1441 agcgcgttgg tcaccaccgt ggtcgcgtgg gacagcagcg atacatcggc cgcagcgatg
     1501 cggttcgtat ccaacccctg ccggattccg tctatcaccg ccaggccgaa atcgtgcggt
     1561 gtggtcagca gcttggcgat ggcgatctgg cccgtctgct cctccaccag agccacgtcg
     1621 gtgaaggtgc ccccaatgtc gatgccgata cgccacgcca tgcgattgtt tcccgtcccg
     1681 cctccgctac aaccgcacta tgcgtgatcc gcgccgcgat ggcacctccc gcctgatgct
     1741 gttcttcgcc gtcgtctata cggtggaggg cttctgccag gcgaagatcg gcgtcatctg
     1801 gcaaccgctg acccactacc tcaaggaaag ccagggctgg gacgcggtac atatcgccac
     1861 ctcgctcgcg gtgctggacg tgccgtgggt agtgaaaccg ctttacggca tcgtctccga
     1921 tttcttcccg ttgttcggct atcgccgccg cagctatctg ttgctggcca acgccgtcgc
     1981 agttgccgtg ttcctatggg ccacgcaaac gcttgcgccc tccatcctca tcttcgcgct
     2041 ggcgctgagc gcgattgcgc tggccatcgc cagcactgtg tgcggcgctc tgctggtgga
     2101 gaacggccag cgaacccatg agagcgccgc attcatcaac cagcagtggc tgtggttcaa
     2161 cgtcgcagtg atggccgcat cgatcatcgg tggcgaattg atcgagtatc tgtcgccggc
     2221 aggcgcgctg cacatggcgc ttggcatagc ggcagctgtg ccagtggccg cactcatcag
     2281 tatcaacctc gtgcacgagg cccacgccgg actcaacatc gccgaattcc gtcacgcgtt
     2341 gcgcggtctg ctggacacgt tccgctcccg caccctgtgg ctcgtcgcgg gcttcctgtt
     2401 tctctactac ttcagccccg gcttcggcac gccgctctat ttctaccaga ccgaccgcct
     2461 gcatttttcg cagggcttca tcggcgtgct ggcttcggtc agtgccgtgg gttgggtggc
     2521 cggggggctg atctatcgat tctggcttgc cggcatgacg acgcgcgcct tgctggtgct
     2581 cagcattatt ggtggcaccc tcggcacgct cacgttcctt ggcatgatcg atgagccgac
     2641 ggcagtcgct gtctatttcc tctatggcgt agcgagtatg gtcgccaaca tcgccaccct
     2701 ttcgctggcg gcggagcact gcccaagggg ctcggaagga ttcaccttcg ccgcattgat
     2761 gtcggtgatc aacctggcga caccgctcgc agataccatc ggcgcctttc tctaccagca
     2821 cgcgttcaat gcgcagcttg caccgctgat cgttgtatcg gcggtggcaa cggcgctgat
     2881 cctcgtgctg ctgccgctgc tggaacgcgc caccgcgcca gcgcgttaga gcccggtgag
     2941 atttggtcga atgacgcgtc gtcccactgc tggtggccac gagctgccag gcttgctgac
     3001 ctagtaacgc gacgccgccc ttcgtgtgtt ctgcgtgttt tgcggttcca ttttgccttg
     3061 ctgtcggtca ggtcgcgccg ggcgaaacca agacaactgg aacccagcgg aattcatccc
     3121 acttcaggca aaaaaagaga gcaggcatgc gctggtcggg gtaccgcaac atgcctgctc
     3181 ccacagacgc catgccgacc agtggggacc gataatacga attcggggag gacgtatatc
     3241 ggactgatcg acactggtgg cgtcggcaga tatgccacca gccgagtggc taattgcgac
     3301 cacaaccaaa gtcgcgttct ctatctatgc cggcatgcgg tgtcaggaaa cgggaaggac
     3361 gccccgccga ttcccactga aaatcccgca ctcggctcac tgggagcacc ctgggattcc
     3421 ccaagaaaat tggccgacga cttaccccga attttctgtt ttgttccgcc taggcggtgc
     3481 ggccgcggcg cggacgcagc acggtgatct cagcaaagcc cggccgccgc tgcgtgctgt
     3541 cagcatcttc gcggcttgct tcgctttgat attcgcacgt catcacatag ttcccgatcc
     3601 gcaagagggc cccatgtggc aacggcgcgc tgccggacag gcgcttgttg tcgacgaacg
     3661 tgccgttggt ggagttcagg tcggtgatac tcacctcgtt gccgtcgacg tcgatgcggc
     3721 aatgcgcgcg cgaaacctcc ggcccttcca gcagaagcgc agagggcggc gcgcggccga
     3781 tggtcaacgc accaggcggc agagcaacgc ggcgcaccgc cgattccggc tgcagaatca
     3841 ccagatggtg caacgactcc ggcggcaccg gccgcagtcg gcccgagcgc agattcagag
     3901 tcgcttcgac ggacggatct tccgcgaacg gcgactcggg ctcctcctcc gtcacgccaa
     3961 gactgcgcgc cacatcagga agtgccgccg cggccgtggc caacgcctga ctgaactctc
     4021 tcgccgcgtc atctgccaac gcgacgcgta gcgacttgcc cggctggcgt tccagcagcc
     4081 caagcccggt caggtaggaa atgtggtcgc gcgtggtctg gaagttctgc cccgtcaggt
     4141 tcagcagcgt ggccgccatc tgatcggcca gaatgccctg ctcgcttccc gccagcgtcg
     4201 atgagccgta ccgcagttcc accgccatat gcagcagcgt ccaatgcgac atcgagttca
     4261 aatggcgaat cgcctcgaca cggcgagcgc gcgacaggtt gctggcgtcg aagatccgtt
     4321 ccagcgcggc ccgccacggt tccctgcacc ggaccagcga ttgcaggatc acgccacgct
     4381 gcgcggcgtc cagccttcgt ggcggcgcca gacgaaccgt cgggtccaac gccgcggcgg
     4441 acgtcgccag ttccttcgcc aacgcaacca ggtgccggtc aaacagatcg agcagcgccg
     4501 gtgtcggaac aatcacggaa cgcgccgaca gcgacccacc cggcggatcg accacacaca
     4561 gtttcagctc ggcgatttcg aggatgcgtt ccttgacggt ggaatcatcc agcaggaaca
     4621 cctgcttgac caaaccgtac agttcgccaa cggcagcccc ttcgcgccct gtcgtgcaga
     4681 gatgcgtgac aatatgccac tgcgcccgtt tgaacaggct cggaaagtgg gcatgccaga
     4741 tcgtctgata ggcggacagg aagcgaacaa cttcgcgctc tgcctgcatc ggcggctccg
     4801 cgtggctccc aaggtttcga cagacgttag actagcaccg tcacgaacgc aaaactgtga
     4861 cctgcttcag cgaaaatgga ttatttcccg cccggggccc gagtggcaga gattttccca
     4921 tcctgcaaat ccgcggtcag ggcggcggga tcgcgctcgc ccggatcgcc gaatccgccg
     4981 cccccacctg tctcgatccg gatcgtctcc cccgcaccaa gcacaaggct ggcggttttc
     5041 gatgacagct cttcctcgcc gttgcggatc aggtgcgccg tggcgccatc cccgccgccc
     5101 gccagccccg gcgcaccaag cacatgccca tcggagcgaa ccgagaacac gatgccgtct
     5161 cgcatcgccc ggatctgccg cgctatgccc agccccccgc gataccgacc ggcccctccg
     5221 gaatcgggta ccagcgcata ctcgtccacc agcagcgcat attcgtgctc cagcgcctcg
     5281 gccggcagat tggaggtgtt ggtcagatgt acgtggatcg catccatgcc atccgcatcg
     5341 ctgcgcgctc ccatcccgcc gcccagcgtc tcgagataca cgaacgtatc gtcgcccacc
     5401 cggcgcggcc cggaaaagat gatggcgggc acgctgtcat gggacgacgc catcacccga
     5461 tcgacgggca acaggcccgc gaaagcgcca aacagcgccc gggcgatctt gttacaggtg
     5521 atgctgcgcg cgcctaccgc cgcgggaaaa cgcggggcga ccagcgtgcc ggattcggct
     5581 ttgatatcga tacagtcgaa catgccgctg ttcggcagca ggttgggatc cagcagcgtc
     5641 ttcaccgcgt aatacaccgt ggcgcgcaaa gcgctctctg gcacgttcag cgcaccccgc
     5701 gtctccggcc cggtaccgga aaaatccacc gtcatcgcct cgccgctgat gaccacccgc
     5761 gcccggatgg gcacgggatc gccaccgagc ccgtcatcat ccagccaggc ggtgaagctg
     5821 ccctcgccat ccgctatgtc acggatgcgg ttgcgcagcc gccgcgcggt gtaagccagc
     5881 agatcgtcaa ccgcctcctc aaccgcagtc agccccatgc gttcaaccag ctcgcgcatc
     5941 agccgcacac cgcgcgtatt ggcagcaacc tgcaccttca gatcgagcga ccgttccacc
     6001 ggatcacgcg agttgtgcgc gatcaggccc agcaggtctt catccagctc ccccgcgcgc
     6061 accagccgga tcaccggcag ccgcagccct tcttcgaaca ccgaccgcgc gcgccccgag
     6121 atggaaccgg gcaccggtcc gccgacatcg gagtgatgcc cgacattggc ggcaaacgcg
     6181 cgcaccgcac catcgatcac aatgggcgtg acaatcgaaa tgtccggcat a
//
LOCUS       DATGEM010000134         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_989788, whole genome shotgun sequence.
ACCESSION   DATGEM010000134 DATGEM010000000
VERSION     DATGEM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989788"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..762)
                     /locus_tag="VKQ27_04280"
     CDS             complement(127..762)
                     /locus_tag="VKQ27_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88179.1"
                     /translation="MQSTRSIFRWMVSGFCVALILSVAVLGLEGIGEKATVAALRVTG
                     RWSFLLFWLAYTGSAMATLLGQSFQPIRRRSREFGLAFASAHLVHLALVAWLCWIGAT
                     PATSVFVLFGIAVFWTYAIALVSFGDPRKMLGSAGWWLLRAVGLNYIAYAFATDFTSN
                     PFSGGITRQIAYVPFAILSVLGPILWVAGEAARFARGRAATAIPAGRPRVP"
     gene            complement(854..1588)
                     /locus_tag="VKQ27_04285"
     CDS             complement(854..1588)
                     /locus_tag="VKQ27_04285"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLY88180.1"
                     /translation="MTTSFDVRHYDSIGSTNDEAVRLANEGAAHGTVVHADQQTVGRG
                     RLSRKWQSPPGNLYVSIILRLDLPAARTMEISFIAALAVADAIETMLPRQERATLKWP
                     NDVLVRDGKISGILLEQAGDALILGIGINVLEPPGGVSYNVSTIVGCGGLATVDGTRS
                     ALLAALANWLDIWQRDGFAAVRSTWLARAHPPGTKLRVVIGDRTIEGRFADLAPDGAL
                     LLDTDEGRKRYVAGDVSLTAPGGAEA"
     gene            complement(1585..2589)
                     /locus_tag="VKQ27_04290"
     CDS             complement(1585..2589)
                     /locus_tag="VKQ27_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY88181.1"
                     /translation="MIAPFINSSDLVPNGPALAQRMRRDGYLFLPGLVPREAIANVQR
                     QIAEIAREAGWLRPDTPVEDTIADPTGFCVDPDPTYLKTLRRINRLEDYHALKHHPAL
                     VNLLERMLGGPILPHPRVLMRNIFPARDEYTTKAHQDFPNVQGTTAVYTAWMPLIDCP
                     MDVGPLQIAAGSHTKGVYDFGIAGGAGGIEIKDPLEGTWVSSDFAAGDVLLFHSMVVH
                     KGVPNHSDRLRMSMDVRYQLVSEPFNIDNANADGQPLSWEDIYADWKSDALKYYWQRL
                     PLTLKPFDPTWFDKRDALGFDLGEARDPRARSVLQRIVARDANPEKRARAQRLLDALP
                     "
     gene            complement(2708..>3380)
                     /locus_tag="VKQ27_04295"
     CDS             complement(2708..>3380)
                     /locus_tag="VKQ27_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY88182.1"
                     /translation="VGISQDNATATAAIDAGAPTHLLVYLQDGTAAVLPADAAAIDMH
                     RSLDRTRPVADIRFNPANATILNAANAPLAAMRRVLDAGRIVLAADTFGAAQHMFDAA
                     LAFVKQRTQFNRIIGSFQAVKHQFADMVTMLEPCRAMVWYAGYAQDALPDEARLTALQ
                     TKAHLGDIGRDIARMTTEAHGGMGFTDLLGLHYWFKRISYNRQMLGSPEQCREEAARL
                     QGWTP"
BASE COUNT          559 a          983 c         1264 g          574 t
ORIGIN      
        1 ttggtcttgg gttcagcctg gacggtcccg gacatgcgtg cctctgttct gcgttgggcg
       61 cagcatggaa cttatgccgc acctgcgaaa gccttttctg cgtgacaggg cggtgcaaat
      121 ccgaggctac ggtacgcgtg gccgcccggc cggtatcgcg gtcgcagcgc gcccgcgggc
      181 gaaacgtgcc gcctcgccgg ccacccacag gatcggtccg aggacggaaa ggatggcgaa
      241 cggaacgtag gcgatctggc gcgtgatacc gccggagaat ggattgctgg tgaaatcggt
      301 cgcgaaggca taggcgatgt aattcagccc caccgcccgc agcagccacc agccagcgga
      361 gcccagcatc ttgcgcgggt cgccaaacga gaccagggcg atggcatagg tccagaagac
      421 ggcgatcccg aacagcacga acactgacgt cgccggggtt gctccgatcc agcacagcca
      481 ggcaaccagc gcgagatgga ccaggtgagc cgacgcaaat gcgaggccga attcgcggct
      541 gcgacgccgg attggctgga agctttggcc aagcagggtg gccatcgcac tgccggtata
      601 ggccagccag aacagcagga atgaccagcg ccccgttacg cgaagcgcgg cgacagtggc
      661 tttctcgcca atgccctcga ggccgagtac ggcgacggac aagatcagtg cgacgcagaa
      721 gcccgaaacc atccatcgga aaatggagcg cgtggactgc atggctggcg tttagcacgc
      781 gctgggacgt tggcggatgg aaactcgtgg ggtcgacctt cagctcacct gaccccacgg
      841 gccaaggctg gggtcacgct tctgcgccgc caggggcggt gagggacacg tcgccggcga
      901 cgtagcgctt gcggccctcg tcggtgtcca gcagcagcgc gccatcgggg gcgaggtcgg
      961 cgaagcggcc ttcgatggtg cggtcgccga tgaccacgcg gagcttggtg ccgggcggat
     1021 gggcgcgggc gagccaggtg gagcggacag cggcgaaacc gtcgcgctgc cagatgtcga
     1081 gccagttggc caaggccgcg agcagcgcgc tgcgagtccc atctaccgtc gccaggccgc
     1141 cgcagccgac aattgttgat acattgtagg atacaccgcc gggaggctcg agcacgttga
     1201 tgccgatgcc gaggatcagg gcgtcgccgg cctgttcgag caggatgccc gagatcttgc
     1261 catcgcgcac cagtacgtcg ttcggccatt tgagcgtggc gcgttcctgc ctgggcagca
     1321 tcgtttcgat cgcgtcggcg accgcgaggg cggcgatgaa gctgatctcc atggtgcgcg
     1381 cggcgggcag gtccaggcga aggatgatcg agacgtagag attaccgggc gggctttgcc
     1441 atttgcgcga gagacggcct cggccgacgg tctgctgatc ggcgtgcacc actgtgccgt
     1501 gcgcggcccc ttcgttggcg agccgcacgg cctcgtcgtt ggtggagcct atactgtcgt
     1561 agtgacggac gtcgaaactg gtggtcacgg cagcgcgtcc agcaggcgtt gagcgcgggc
     1621 ccgtttctcc gggttggcgt cacgggcgac gatacgctgc aggacggacc gggcgcgggg
     1681 atcgcgggcc tcgccgaggt cgaagccgag cgcatcgcgc ttgtcgaacc aggtcgggtc
     1741 gaagggtttc agcgtgaggg ggaggcgctg ccagtagtat ttgagcgcgt cggacttcca
     1801 gtcggcgtag atgtcttccc aggagagcgg ctggccgtcg gcattggcgt tgtcgatgtt
     1861 gaagggctcg ctgacgagtt ggtagcgcac gtccatcgac atgcgcagcc tgtcgctgtg
     1921 gttggggacg cctttgtgga cgaccatgct gtggaacagc agcacgtcgc cggccgcgaa
     1981 gtcgctggag acccaggtgc cttccagcgg gtccttgatc tcgatgccgc cggcgccacc
     2041 ggcgatgccg aaatcgtaga cgcccttggt gtgggagccg gcggcgatct gcagcgggcc
     2101 gacatccatc gggcagtcta tcagcggcat ccaggctgta tataccgccg tggtcccctg
     2161 gacgttgggg aaatcctggt gggccttggt ggtgtattcg tcgcgcgcgg ggaagatgtt
     2221 gcgcatcagc acgcgcgggt gcggcaggat ggggccgccg agcatgcgct ccagcaggtt
     2281 gaccagagcg gggtggtgct tgagggcgtg gtagtcctcg agccggttga tgcggcggag
     2341 ggtctttagg tacgtgggat cgggatcgac gcagaagccg gtgggatcgg caatggtgtc
     2401 ttccaccggc gtgtcggggc ggagccagcc ggcttcgcgg gcgatctcgg cgatctggcg
     2461 ctggacgttg gcgatggctt cgcgggggac gaggccgggc aggaacagat agccgtcgcg
     2521 gcgcatgcgt tgggcgaggg cggggccgtt gggcaccaga tcggaagagt tgataaatgg
     2581 cgcgatcatg ccgggccttc tgtcattgcg agcagagcgc agcaatccca gtcgtcctgc
     2641 gcgcgcagag accgaccgag attgcttcgc ttcgctcgca atgacaaggg acgactcgga
     2701 catcgactta tggggtccat ccttggaggc gtgcggcttc ctcgcggcat tgctcggggc
     2761 tgccgagcat ctggcgattg tagctaatgc gcttgaacca gtagtgcagg ccgagcaggt
     2821 cggtgaagcc catgccgccg tgcgcctcgg tggtcatgcg cgcgatatcg cggccgatgt
     2881 cgccgagatg cgccttggtc tgcagcgcag tgaggcgggc ttcatcgggc aacgcgtcct
     2941 gcgcgtagcc ggcgtaccag accatggcgc ggcagggttc cagcatggtc accatgtcgg
     3001 cgaactggtg cttcaccgcc tggaaactgc cgatgatgcg gttgaactgc gtgcgctgct
     3061 tcacaaaggc gagtgcggca tcgaacatgt gctgcgcggc gccgaacgtg tcggcggcaa
     3121 gcacgatgcg gccggcgtcc agcacgcgcc gcatggcggc cagcggcgcg ttggcggcat
     3181 tcaggatggt ggcgttggcc gggttgaagc ggatgtcggc gaccgggcgc gtgcggtcga
     3241 ggctgcgatg catgtcgatg gcggcggcgt cggcagggag cacggcggcg gtgccgtctt
     3301 gtagatacac cagtagatgc gtgggggcgc cggcgtcgat cgccgcggtg gcggtggcgt
     3361 tgtcctggct gatgccgacg
//
LOCUS       DATGEM010000135         7777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1225833, whole genome shotgun sequence.
ACCESSION   DATGEM010000135 DATGEM010000000
VERSION     DATGEM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225833"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2226
                     /gene="nuoG"
                     /locus_tag="VKQ27_04300"
     CDS             <1..2226
                     /gene="nuoG"
                     /locus_tag="VKQ27_04300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HLY88183.1"
                     /translation="DTVNIEVNGVALKARKNELIMRVTDREGIYVPRFCYHEKLAVAA
                     NCRMCLVEVEKAPKPMPACATPVTEGMKVFTKSPKAVAAQRATMEFLLINHPLDCPIC
                     DQGGECELQDLAMGFGRDIARFSERKRVVKDKNLGPLISTDMTRCIHCTRCVRFGLEI
                     QGYPQMGTTGRGEHMEVGTYIEQSVDHELSANIIDLCPVGALNNKPFRYHARAWEMQQ
                     QPLVSPHDAFGTNLYAHTLRGRIMRMVPRENEAINETWIADRDRFGFEGIYSGERVTQ
                     PLMRVDGALQEVEWEAALTAAAEGLQRTIAGHGAASAGFLSSPTATVEEMYLLARIAR
                     GLGSGNVDHRLRQLDFRAQETESAYPSLGMPIAGVDLLEGVLIIGSNLRHEMPMLAHR
                     IRKAALKGAKVAFLNPRVFEYMFPVAAYGLAEHDLVGNLAAVVHAAAAAANQPVPAGL
                     PAGATVTDDHRSVAAVLAQGTRRAVLLGNLAQRHPAYSELKRLGGLLASLTGARLALL
                     TEGPNAAGAYLAGAVPHRGPGGATVQPGLSARAMLDSALKAYVLFGGIDPALDLGAGA
                     DSLRSADFVVAATTHLPASLREVAHVVLPIGSFAESSGTYVNIEGRWQSWAGAAKLLG
                     DSRPGWKVLRVLANLLAVPGVDYVSSEEVREALRSACGEGLTTPNGGAGGTAPGAAAG
                     SAAAASATRGGAGGGGAANGAAVPTGPWIDIPPYEIDVLVRGSEALGKTKDGRLARDV
                     I"
     gene            2229..3308
                     /gene="nuoH"
                     /locus_tag="VKQ27_04305"
     CDS             2229..3308
                     /gene="nuoH"
                     /locus_tag="VKQ27_04305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HLY88184.1"
                     /translation="MWDWSPIREWLSERITPYWTFPLLTAVQALIVVVCIILTMAYYT
                     LAERKIIGWIQSRKGPNRVNLFWIPGAGQPFADVFKLLFKEILIPADASGFLFRLAPF
                     LSMIPAIAIWAVIPLSPERAFANIDAGLLYVLALTSMGVYGIILAGWAANSKYAFLGA
                     MRSAAQIVAYEIAMGFAMVGVLMAAGSLNIGDIIQAQHGGGLSWNWFWLFPLLLVYFI
                     SGVAETNRAPFDVAEGESEIVAGFHVDYSGMAFALFFIAEYVNMILISALTVVFFFGG
                     WLSPFDGYLPEDAWLASPSFFWFGLKTLFPMFSFLWLRATFPRYRYDQIMRLGWKALI
                     PVTLVWLLAEGCMVYFHVGPWKGHP"
     gene            3308..3796
                     /gene="nuoI"
                     /locus_tag="VKQ27_04310"
     CDS             3308..3796
                     /gene="nuoI"
                     /locus_tag="VKQ27_04310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HLY88185.1"
                     /translation="MQGIRSFFKTFLLWELLLGLWVTLRQMFQRKVTINYPEEKTPQS
                     SRFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESDVGSDGTRRTTRYDIDLFKC
                     IYCGFCEEACPVDAIVETRLHEYHMENRGENIMSKDKLLGIGERYEAMIAADRAADAR
                     YR"
     gene            3796..4398
                     /locus_tag="VKQ27_04315"
     CDS             3796..4398
                     /locus_tag="VKQ27_04315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005164.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLY88186.1"
                     /translation="MFPAILFFLFAAVLICAALGVILARNPVHCALLLVLCFFNSAVI
                     WLLLDAEFLGIVLVLVYVGAVMVLFLFVVMMIDVNVEEFRKNLAGYWPLAVAVAGFVV
                     FAIINVIVVRHLGGIALKTAPALPQHYSNTRTLGIALFTQYAYPAQVAACILLVASVA
                     AIILTLRRRAGGKPQDIARQVAVRAKDRVRLVKMASEKRT"
     gene            4395..4700
                     /gene="nuoK"
                     /locus_tag="VKQ27_04320"
     CDS             4395..4700
                     /gene="nuoK"
                     /locus_tag="VKQ27_04320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008560402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HLY88187.1"
                     /translation="MITLTHVLALSGVLFAIAVAGVFLNRRNVIVLLMCIELMLLAVN
                     FNFVAFSRFLGDISGQVFVFFILTVAAAEAAIGLAILVVLFRERRSIDVEDLDTLQG"
     gene            4738..6693
                     /gene="nuoL"
                     /locus_tag="VKQ27_04325"
     CDS             4738..6693
                     /gene="nuoL"
                     /locus_tag="VKQ27_04325"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HLY88188.1"
                     /translation="MQSIYLTIALAPLVAAIIAGLFGRIIGRAGAHSITIAAVALSCG
                     LSMYVLYEMLAGGMAPFNGPVYTWLVSDGLTMEVGFLIDHLSAMMMVVVSFVSLMVHV
                     YTIGYMHDDDGYQRFFSYISLFTFSMLMLVMSNNFMQLFFGWEAVGLVSYLLIGFWYT
                     RPSAVFANLKAFVVNRIGDFGFVLGIAAILRYTGSLDYATVFAHTDAFAGAQIHISGT
                     ITWSAITFTCICLFIGAMGKSAQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVA
                     RMSPLFELSESALSFVLVIGATTALFMGLLGIVNNDIKRVIAYSTLSQLGYMTVALGV
                     SAYGAGIFHLMTHAFFKALLFLAAGSVIIAMHHEQDMRKMGGLAKHMPITYWTCLVGC
                     LALIGFPGTAGFFSKDSLIDAVGASHRFGSGYAYVCVVGGVFITALYTFRMFIMTFLG
                     KERMDHHTLEHLHESPWVVWVPLVLLAIPSAIIGWVTVGPVLFGNFFEGSIVVLPANN
                     VIEHVAEEFHGPGAFVVSALTAPPVWLAAAGVLTAYIFFKWKPAWADSAERIFKWPHT
                     ILVEKYGFDWFNENVIAAAARGLGGGLWRFGDQDLIDDGLVNGSARVVAWFSSVMRYA
                     QSGYLYHYAFAMILGLASLLLWLIWRP"
     gene            6693..>7777
                     /locus_tag="VKQ27_04330"
     CDS             6693..>7777
                     /locus_tag="VKQ27_04330"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLY88189.1"
                     /translation="MLQDHLLEVLIWLPILGGFLVLGLGDRPPAARWVSLGVSIVTFA
                     FSIPLWAWFKTGTAEMQFAETGPWIPAIHSNFHIGVDGISMPLILLTTFTTVLIVVAS
                     WQNVEKRIAQYFAAFLILEGLMIGVFAALDGVLFYVFWEAMLIPMFIIIGIWGGPRRV
                     YATIKFFLYTFIGSLLMLVALIYLYLKSGSYELAVFQRMPLTLTEQTLIFLAFFAAFA
                     VKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKIGGYGFLRFSLPIVPDACQEFAWLI
                     LALSLIAVIYVGFVALVQEDMKKLIAYSSVSHMGFVTLGTFIALALVRVTGNKDAAYL
                     GVSGAMVQMISHGFVSGAMFSCVGVLY"
BASE COUNT         1207 a         2447 c         2539 g         1584 t
ORIGIN      
        1 gataccgtca acatcgaagt caacggcgtc gcgctcaagg ctcgcaagaa cgagctcatc
       61 atgcgcgtca ccgatcgcga aggcatctac gtgccgcgct tctgctatca cgagaagctc
      121 gccgtcgccg ccaactgccg catgtgcctg gtcgaggtcg agaaggcgcc gaagccgatg
      181 ccggcgtgcg cgaccccggt gaccgagggc atgaaggtgt tcaccaaatc gccgaaagcc
      241 gtcgccgccc agcgcgcgac gatggagttc ctgctcatca atcatccgct cgattgcccg
      301 atctgcgatc agggcggcga gtgcgaactg caggatctcg cgatgggctt cggccgcgac
      361 atcgcgcgct tcagcgaacg caaacgggtc gtgaaggaca agaacctcgg gcccctgatc
      421 tcgaccgaca tgacgcgctg cattcattgc acccgctgcg tgcgcttcgg cctggagatc
      481 caaggctatc cgcagatggg cacgaccggc cgcggcgagc acatggaagt gggcacctac
      541 atcgaacaga gcgtcgatca cgaactgtcg gccaacatca tcgatctgtg ccccgtgggg
      601 gcgctcaaca acaagccgtt ccgctatcac gcgcgcgcct gggagatgca gcagcagcca
      661 ttggtgtcgc cgcacgatgc gttcggcacc aatctctatg cgcatacgct gcgcggcagg
      721 atcatgcgca tggtgccgcg ggaaaacgag gccatcaacg agacctggat cgcggaccgc
      781 gaccgcttcg gcttcgaggg catctactcc ggcgagcggg tcacgcagcc gctgatgcgg
      841 gtcgacggcg cgcttcagga ggtcgagtgg gaagcggcat tgaccgcggc ggcggaagga
      901 ttgcagagga ccatcgccgg ccacggggcc gcgtccgcgg ggtttctatc atcgcccacc
      961 gccacggtcg aggaaatgta tctattggcg cggattgccc gcggcctggg cagcggcaac
     1021 gtcgatcacc gcctgcgtca actcgatttt cgagcgcagg agaccgagag cgcatatccg
     1081 agcctcggca tgccgatcgc cggcgtcgac ctgttggagg gtgtgctcat catcggctcg
     1141 aacctgcgcc atgagatgcc gatgctggcg caccggatcc gcaaagcggc gctcaagggc
     1201 gccaaggtgg ctttcctcaa tccgcgcgta ttcgaatata tgtttccggt cgccgcctac
     1261 gggctcgccg agcacgatct ggtcggcaat ctcgcagccg tcgtgcatgc ggccgcggcg
     1321 gcggcgaacc agcccgttcc ggccggcctt ccggcgggcg cgacggtcac cgacgatcat
     1381 cggtccgtgg ccgccgtgct cgcgcagggc acccgtcgcg cggtgctgct cggcaatctc
     1441 gcgcagcggc atcccgccta ctcggaactg aagcggctgg gcggcctgct ggcgtcgttg
     1501 accggcgcgc ggctcgcgct cctgaccgag gggcccaatg ccgcgggagc ctatctggcg
     1561 ggagccgtgc cccaccgcgg gcccggcggg gcaacggtcc agccggggct ctcggcgcgc
     1621 gcgatgctgg attccgcgct caaggcctac gtgctgttcg gcggcatcga tcccgccctc
     1681 gatctcggag ccggtgcgga ctcgctgcgc agcgccgact ttgtggtggc ggccacgacc
     1741 cacttgccgg cgagtctgcg cgaggtggcg catgtcgtcc tgcccatcgg gtcgttcgcc
     1801 gagtcgtcgg gcacctatgt caacatcgag ggccgctggc agagttgggc gggtgccgca
     1861 aaactgctcg gcgacagccg gccgggatgg aaagtgctgc gggtattggc gaatctcctc
     1921 gccgttcccg gcgtcgacta cgtgagctcc gaggaagtcc gcgaggcgct ccgcagcgcc
     1981 tgcggagaag gcttgacgac gcccaatggc ggcgccggcg gcacggcacc gggagcggcc
     2041 gctggcagcg cggcggcggc cagcgcgaca cgtggcgggg cgggaggagg cggcgcggcc
     2101 aatggtgcgg cggtgccgac cggcccctgg atcgacattc cgccctacga gatcgacgtg
     2161 ctggtgcgcg gatccgaagc gctcggcaag accaaggacg ggcgcttggc ccgggatgtg
     2221 atctagccat gtgggattgg agcccgattc gcgagtggct gtccgagcgg atcacgccgt
     2281 actggacctt cccgctgctg acggcggttc aggcgctgat cgtggtggtg tgcatcatcc
     2341 tgaccatggc gtactacacg ctggcggagc gcaagatcat cggctggatt cagtcgcgca
     2401 agggaccgaa ccgggtcaat ctgttctgga ttcccggcgc cgggcagccg ttcgcggacg
     2461 tgttcaagct gctcttcaag gaaattctga tcccggccga tgccagcggg ttcttgttcc
     2521 ggctggcgcc gttcctgtcg atgataccgg cgatcgcgat ctgggccgtc attccattgt
     2581 cgcccgagcg cgcgttcgcg aacatcgatg cgggcctcct gtatgttctg gcgctcacgt
     2641 cgatgggcgt gtacggcatc atcctcgccg gctgggccgc gaactcgaag tacgcgttcc
     2701 tcggcgccat gcgttcggcc gcccagatcg tcgcgtatga aatcgccatg ggcttcgcca
     2761 tggtgggtgt gttgatggcg gcgggcagcc tcaacatcgg cgacatcatc caggcgcagc
     2821 atggcggcgg cctgagctgg aactggttct ggctgttccc gctgctgctg gtgtacttca
     2881 tttcgggcgt ggcggagacc aaccgcgccc ccttcgacgt cgccgagggc gagtcggaaa
     2941 tcgtcgccgg gttccacgtc gactattcgg ggatggcgtt cgcgctgttc ttcatcgccg
     3001 aatacgtcaa catgatcctg atctcggcac tgacggtggt gttcttcttc ggcgggtggc
     3061 tgtcgccgtt cgacggctac ctgcccgagg acgcgtggct cgccagcccc agcttcttct
     3121 ggttcggact gaagaccctg tttcccatgt tcagtttcct gtggctgcgc gcgacctttc
     3181 cgcgctaccg ctatgaccaa atcatgcggc tgggttggaa ggcgttgatc ccggtgacgc
     3241 tggtctggtt gttggccgag ggctgcatgg tgtacttcca tgtgggaccg tggaaggggc
     3301 acccgtgatg cagggcatac gcagtttctt caagacgttc cttctctggg agctgctgct
     3361 gggcctgtgg gtgaccctgc gccagatgtt ccagcgcaag gtcacgatca attacccgga
     3421 agagaagacg ccgcaaagct cccgctttcg cgggctgcat gcgctgcgcc ggtaccccaa
     3481 cggcgaggag cgctgcatcg cctgcaagct gtgcgaagcg gtgtgcccgg cgctggcgat
     3541 caccatcgag tcggacgtcg gcagcgacgg cacgcggcgc accacgcgct acgacatcga
     3601 cctgttcaag tgcatctatt gcgggttctg cgaggaggcc tgtccggtcg atgccatcgt
     3661 cgagacgcgg ctgcacgaat atcacatgga aaaccgcggc gagaacatca tgagcaagga
     3721 caagctgctc gggatcggcg aacgttacga agccatgatt gcggcggatc gcgccgccga
     3781 tgcgcggtac cgctgatgtt tccggcgatt ctcttcttcc tgttcgccgc ggtgctgatc
     3841 tgcgccgcgc tcggcgtgat cctggcgcgc aatcccgtcc actgcgcgct gctcctggtg
     3901 ctgtgcttct tcaatagcgc ggtcatctgg ctgctgctcg acgcggagtt cctggggatc
     3961 gtgctggtgc tggtgtacgt cggcgccgtg atggtgctgt tcctgttcgt ggtcatgatg
     4021 atcgacgtca acgtcgagga gttccgcaaa aacctggcgg gctactggcc cctggcggtg
     4081 gccgtggccg gcttcgtcgt tttcgccatc atcaacgtga tcgtggtcag gcacctgggc
     4141 ggcatcgccc tgaaaaccgc ccccgcgctg ccgcagcact actccaatac ccgcacgctg
     4201 gggatcgccc tgttcacgca atacgcgtac cccgcccagg tggcggcgtg catcctgctc
     4261 gtggccagcg tcgccgccat catcctgacc ttgcgccgcc gcgccggcgg caagccccag
     4321 gacatcgcac ggcaggtggc cgtgcgtgcg aaggaccggg tacggctcgt caaaatggcc
     4381 tcagagaaac gtacgtgatc accctcactc atgtgctggc gctgtccggc gtgctgttcg
     4441 ccatcgccgt tgccggcgtg ttcctgaatc gcaggaacgt catcgtgctc ctgatgtgca
     4501 tcgagctgat gctgctcgcc gtcaacttca acttcgtggc gttctcgcgc tttctcggcg
     4561 acatcagcgg ccaggtcttc gtgttcttca tcttgaccgt cgccgcggct gaagcggcga
     4621 tcgggctggc catcctggtc gtgctgttcc gggaacgcag gtccatcgac gtcgaagatc
     4681 tcgacaccct gcagggctga accggcagat aagaagggat aaggaaaccg aagaaaaatg
     4741 cagtcgatct accttacgat tgcgcttgcg cccctggtcg cagcgatcat cgcgggcctg
     4801 ttcggccgga tcatcggccg cgcgggggcg cacagcatca ccatcgccgc ggtcgcgctg
     4861 tcctgcggcc tgtcgatgta cgtcttgtat gaaatgctcg ccgggggcat ggcgcccttc
     4921 aacgggccgg tttacacctg gctggtgagc gacggcctga cgatggaagt gggatttctg
     4981 atcgaccatc tctcggcgat gatgatggtg gtggtgtcct tcgtatccct gatggtgcac
     5041 gtctacacca tcggctacat gcacgacgac gacggctacc agcgcttctt cagctacatc
     5101 tccctgttca ccttttccat gctgatgctg gtgatgtcga acaacttcat gcagctgttc
     5161 ttcggctggg aagcggtggg gctggtgtcg tacctgctca tcggattctg gtacacgcgg
     5221 ccgtcggccg tgttcgccaa tctcaaggcc ttcgtggtca atcggatcgg cgatttcggg
     5281 ttcgtgctgg gtatcgccgc gattctgcgc tacacggggt cgctcgacta tgcgacggtg
     5341 ttcgcgcaca ccgatgcctt cgccggcgcc cagatccaca tcagtggcac aataacctgg
     5401 tcggcgatta cgttcacgtg catctgcctc ttcatcggcg ccatgggcaa atccgcgcag
     5461 gtcccgctgc acgtatggct gcccgattcc atggagggtc cgacgccgat ttcggcgttg
     5521 atccacgccg ccaccatggt gacggccggc atcttcatgg tggcgaggat gtcgccgctg
     5581 ttcgagctgt ccgagtcggc actgtcgttc gtgctcgtca tcggcgccac caccgcgctc
     5641 ttcatggggc ttctgggaat cgtcaataac gacatcaagc gcgtgattgc ctattcgacc
     5701 ttgtcccaac tcgggtacat gacggtggcg ctcggcgtct cggcgtacgg cgcggggata
     5761 tttcacctga tgacgcatgc gttcttcaag gcgcttctgt tccttgcggc gggctcggtg
     5821 atcatcgcca tgcatcatga acaggacatg cgcaagatgg gcggcctggc caagcacatg
     5881 ccgatcacct actggacctg cctggtgggt tgcctggcgc tcatcggctt cccgggcacc
     5941 gcgggcttct tctccaagga ttcgctgatc gacgcggtcg gggcgtcgca tcggttcgga
     6001 tcgggctacg cgtacgtgtg cgtggtgggc ggggtgttca tcacggcgct gtacaccttc
     6061 cgcatgttca tcatgacctt cctcggcaag gaacggatgg atcaccacac gctggaacac
     6121 ttgcacgaga gtccgtgggt cgtgtgggtg ccgctcgtgc tgctggccat tccgtccgcg
     6181 atcatcgggt gggtcacggt ggggcccgtg cttttcggaa acttcttcga agggtcgatc
     6241 gtggtgctgc cggccaacaa cgtgatcgaa cacgtggccg aggagtttca cggtcccggc
     6301 gcattcgtcg tgtcggccct gacggcgccg ccggtatggc tcgctgcggc aggggtcttg
     6361 accgcctaca tcttcttcaa gtggaagccc gcttgggcgg actcggctga gcgaatattc
     6421 aagtggccgc acaccatttt ggtggagaag tacggcttcg actggttcaa tgaaaacgtc
     6481 atcgccgccg ccgccagggg cttgggcggc gggctgtggc gtttcggcga ccaggacttg
     6541 atcgacgatg ggctggtcaa cggcagcgcc cgcgtggtcg cgtggttcag ttcggtgatg
     6601 cgctacgcgc agtccggata tctctatcac tacgcattcg ccatgattct gggcctggcc
     6661 tcgctgctgc tgtggctgat ctggcgtcca taatgctgca agaccatctg ctcgaggtcc
     6721 tgatctggct gcccatcctc ggcggcttcc tcgtcctggg tttgggcgat cgtcctcccg
     6781 ccgcgcgctg ggtgtccttg ggagtttcca tcgtcacctt cgccttcagc atcccgctgt
     6841 gggcatggtt caagaccggg acggcggaga tgcaattcgc tgaaacggga ccgtggattc
     6901 ccgccattca ctcgaacttt cacatcggcg tcgacgggat ttcgatgccg ctgatcctgt
     6961 tgaccacgtt caccacggta ctgatcgtgg tcgcgagctg gcagaacgtc gagaagcgca
     7021 tcgcgcaata cttcgccgcc ttcctgatcc tggaaggact gatgatcggc gtgttcgcgg
     7081 cgctcgacgg cgtgctgttc tatgtcttct gggaggcgat gctcattccg atgttcatca
     7141 tcatcggcat ctggggcggc ccgcgccgcg tctatgcgac catcaagttc ttcctgtaca
     7201 cgttcatcgg ctcgctgctc atgctggtgg cgttgatcta cctgtacctg aaatcgggca
     7261 gctacgaact cgcggtgttc cagcgcatgc cgttgacgct caccgagcag accttgatct
     7321 tcctcgcgtt cttcgcggcc ttcgcggtga aggtgccgat gtggccggtg cacacgtggt
     7381 tgccggacgc gcacgtcgaa gcgccgaccg gcggttcggt cgtactggcc gcgatcatgc
     7441 tgaagatcgg cggctacggc ttcctgcgct tctcgctgcc gatcgtgccg gacgcgtgcc
     7501 aggaattcgc ctggctgatc ctcgcgctgt cgctgatcgc cgtgatctac gtcggcttcg
     7561 tcgcgctggt gcaggaggac atgaagaagc tgatcgcgta ctcgtccgtg tcgcacatgg
     7621 gcttcgtcac gctcggcacg ttcatcgcgc tcgcgctggt gcgcgtgacc ggcaacaagg
     7681 acgccgcgta cctcggcgtg tccggcgcga tggtgcagat gatctcgcac ggcttcgtct
     7741 cgggcgcgat gttctcctgc gtcggcgtgc tctacga
//
LOCUS       DATGEM010000136         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_759104, whole genome shotgun sequence.
ACCESSION   DATGEM010000136 DATGEM010000000
VERSION     DATGEM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759104"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..839
                     /locus_tag="VKQ27_04335"
     CDS             join(79..415,415..839)
                     /locus_tag="VKQ27_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612221.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HLY88190.1"
                     /translation="MAARLFMLSDEEWARIEPHLPRGRRGAHRVDDRRVISGIIHMLR
                     TGARWRDCPVEYGPYTTVYNRFNRWSAQGVWEDVFYALSGSSGVVSTTSIDSTHVKAH
                     RSAAGAKGGTFEQAIGRSRGGRTTKIHALADDVGRPIAFSVTPGQTHDLVGAGALLKR
                     IPTPKRLLADRAYDARRLRDWLAERGCEAVIPPNPTRKNPHPYDRTAYRRRNVIERMF
                     CRLKDFRRIATRYDKRADIFLAAVFIVAALVWWAN"
     gene            complement(857..1603)
                     /gene="ubiG"
                     /locus_tag="VKQ27_04340"
     CDS             complement(857..1603)
                     /gene="ubiG"
                     /locus_tag="VKQ27_04340"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HLY88191.1"
                     /translation="MPRAQAHAQVTTASEQEVARFQAIADAWWDSTGSFKPLHQINRP
                     RLEALTDHLAAHFGRDRTADRPFEGLTLIDIGCGGGLLCEPMARLGFSVTGIDAGERN
                     IQVAQHHAEQMGVAIDYRTATPEQVEGQFDVVLSMEVIEHVSDVDAFLAACAELLKPG
                     GAFCGATLNRTGKSFALAIVGAEYVLRWLPRGTHDWRKFVRPSEFSAGLRKVGITVKA
                     LEGVAYDPWRDLWNRVSSVDVNYFLYGVKA"
     gene            1687..2907
                     /locus_tag="VKQ27_04345"
     CDS             1687..2907
                     /locus_tag="VKQ27_04345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLY88192.1"
                     /translation="MARIVMKFGGTSVGDCDRIKNVARRVKSEFEAGNQIAVVVSAMS
                     GVTNQLVEYCRQIAPLHDAREYDVVVSTGEQVTIGLLAIALRELGLRARSWTGWQVPI
                     RTDDAHGKARIETIETEELLAAMEGGQIAVVAGFQGIGPENRITTLGRGGSDTSAVAL
                     AAALNADRCDIYTDVDGVYTTDPRIVAKARKLDKISYEEMLELASVGAKVLQTRSVEM
                     AMKHRVRVQVLSSFEDKPGTLVCDEDEIVEKELVSGIAYSRDEAKITVIKVPDRPGIA
                     ASVFGPLAEANVNVDMIVQNVSEDGRFTDLTFTVTRADFDRAIQLLTDKKGELGYERL
                     MTDKNVVKVSVIGMGMRSHAGVAQKMFSTLAEKGINIQVISTSEIKVSVLLGEDYTEL
                     AVRALHSAYGLDAN"
     gene            2945..>3277
                     /locus_tag="VKQ27_04350"
     CDS             2945..>3277
                     /locus_tag="VKQ27_04350"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLY88193.1"
                     /translation="MSTAKARLEHLWRRGREFLGTETAILGGAMSWVSERNLVAAISN
                     AGGFGVIACGSMSPELLAREIEGTQALTKKPFGVNLITMHPQLSELIDVCIKHKVGYV
                     VLAGGLPTA"
BASE COUNT          643 a          998 c         1032 g          604 t
ORIGIN      
        1 tagggtccgt actcaattag aggattcccg ataaggccgt gttgtgattc aagattcctg
       61 ttggatcagg aggcttgaat ggcggcacgg cttttcatgt tgagcgacga ggaatgggct
      121 cggattgagc cccatcttcc acgcggtcgt cgaggtgcgc atagagtaga cgatcgccgt
      181 gtaatttctg gcatcatcca catgctccgg acaggggcac gctggcgcga ctgcccggtt
      241 gagtacggcc cctacacgac ggtctataac cgcttcaatc gctggagcgc ccaaggggtg
      301 tgggaggacg tcttctatgc cctgagcggt tcgagcggcg tggtttctac aacctcgatc
      361 gactctaccc acgttaaggc gcaccgctcg gcggcgggcg caaaaggggg gacttcgaac
      421 aagccatcgg acgatctcgc ggcggacgga cgactaaaat tcacgcgtta gccgacgatg
      481 tcggccggcc tattgcgttt agcgtcaccc ccggtcagac gcatgatctc gtcggtgccg
      541 gtgcgttgtt aaagcgcatt cccacaccaa agcggctgct cgcagataga gcctatgacg
      601 cgcgaaggct gcgagactgg ctcgctgagc gtggctgtga ggctgtcatt ccgccaaacc
      661 caacccggaa gaacccccat ccttacgacc ggacagctta tcgacgacgc aatgtgatcg
      721 agcgaatgtt ctgtcggctt aaggacttca gaaggatcgc aacacgctac gacaagaggg
      781 cggacatctt cttggccgcc gtcttcatcg tcgctgcgct cgtttggtgg gctaattgag
      841 tccggaccct agtgggctaa gccttcacgc catacagaaa ataattcaca tccacagaag
      901 aaacccggtt ccacagatcc cgccacggat cataagcgac gccttcgagc gccttcaccg
      961 tgatgccgac cttccgcaac cccgccgaaa actccgacgg gcggacgaac tttcgccagt
     1021 catgcgtgcc gcgcggcagc cagcgcagca catattcggc gccgacgatg gccaaggcga
     1081 aggatttgcc ggtgcggttc agggtcgcgc cgcagaaggc cccgcctggc ttcaagagct
     1141 cggcgcaggc ggccaggaag gcgtcgacat ccgagacatg ctcgatcacc tccatcgaca
     1201 gcaccacgtc gaactggccc tcgacctgtt cgggcgtcgc ggtgcggtaa tcgatggcga
     1261 cgcccatctg ctccgcatga tgctgggcta cctggatatt gcgctcgccg gcatcgatgc
     1321 cggtgaccga aaagcctagg cgcgccatcg gctcgcacaa caatcccccg ccgcagccga
     1381 tatcgatcag ggtcagcccc tcgaagggac gatcggcggt acgatcgcgg ccgaaatggg
     1441 cggccagatg gtcggtcagg gcttcgaggc gcgggcggtt gatctgatgc aacggcttga
     1501 acgagcccgt gctgtcccac caggcgtcgg cgatggcctg gaagcgggcc acctcctgtt
     1561 cggaagcggt ggtgacttgg gcgtgggctt gcgcgcgcgg catgttgcgt ccttgttaac
     1621 tgttagagta tgtatacccg cccctttcac cggggagcaa tccggcttta ctctcaggac
     1681 cttccaatgg cccgtattgt catgaaattc ggcggcacgt cggttggcga ttgtgaccgc
     1741 atcaagaatg tcgcgcgccg cgtcaaaagc gaattcgagg ccggcaacca gattgccgtc
     1801 gtcgtttcgg cgatgtcggg cgtcaccaac caactggtcg agtattgccg ccagatcgcg
     1861 ccgttgcatg atgcgcgcga atatgacgtc gtcgtgtcga ccggcgagca ggtaaccatc
     1921 ggcctgctgg ccatagcgct gcgcgagctc gggctgcgcg cccgctcctg gaccggctgg
     1981 caggtgccga ttcgcaccga cgacgcccat ggcaaggccc gtatcgaaac catcgagacc
     2041 gaggaattgc tcgcggcgat ggaaggcggc cagatcgccg tggtggcggg cttccagggc
     2101 atcggccccg agaaccgcat caccactttg ggccgcggcg gctcggatac cagcgccgtg
     2161 gccttggccg ccgcgttgaa cgcggatcgc tgcgacatct acaccgacgt cgatggtgtt
     2221 tacaccaccg atccgcgaat cgttgccaag gcccgcaagc tcgataaaat cagctatgaa
     2281 gagatgctgg aactggcatc ggtgggcgcc aaggtgctgc agacgcgctc ggtcgagatg
     2341 gcgatgaagc acagggttcg cgtccaggtg ctgtccagct tcgaggacaa gcccggcacg
     2401 ctggtttgtg atgaggatga aatcgtggaa aaagaactgg tcagcggaat tgcctatagc
     2461 cgggacgaag ccaagatcac cgtgatcaag gtgccggacc gcccgggcat cgccgcctcg
     2521 gtgttcggcc cgctggccga ggccaatgtc aatgtcgaca tgatcgtgca gaacgtgtcc
     2581 gaggacggcc gtttcaccga tctgaccttc accgtgacgc gcgccgattt cgatcgcgcc
     2641 atccagctct tgaccgacaa gaagggcgaa ctgggctatg agcgcctgat gaccgacaag
     2701 aacgtcgtca aggtgtcggt gatcggcatg ggcatgcgca gccatgccgg cgtcgcccag
     2761 aagatgttca gtacgctggc cgaaaaaggg atcaatatcc aggtgatctc gacctccgag
     2821 atcaaggtca gcgtgctgct gggcgaggat tacaccgagc tggccgtccg cgccctgcat
     2881 agcgcctacg gtctcgatgc caactgagat caataccgcc tcgaccgatg cgggggacaa
     2941 gagcatgagc acagccaagg cgcgccttga gcatttgtgg cggcgcggcc gcgaattcct
     3001 cggcaccgag accgccatcc tgggcggcgc gatgtcgtgg gtttccgagc gcaatctggt
     3061 ggcggcaatc tcgaatgccg gcggcttcgg cgtgatcgct tgcgggtcga tgtcccccga
     3121 gctgctggcg cgcgagatcg agggcaccca ggccctgacc aagaagccct tcggcgtcaa
     3181 tctgatcacc atgcatcccc agttgtccga gctgatcgat gtctgcatca agcacaaggt
     3241 cggctatgtc gtgctggccg gcggcctgcc caccgcg
//
LOCUS       DATGEM010000137         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_643625, whole genome shotgun sequence.
ACCESSION   DATGEM010000137 DATGEM010000000
VERSION     DATGEM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643625"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VKQ27_04355"
     CDS             <1..191
                     /locus_tag="VKQ27_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HLY88194.1"
                     /translation="VGTCRMGEGPAAVVDARLRVHGMGGLRVIDASVMPTLTTGNTNA
                     PTIMIAEKGAAMIREDAA"
     gene            complement(200..457)
                     /locus_tag="VKQ27_04360"
     CDS             complement(200..457)
                     /locus_tag="VKQ27_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88195.1"
                     /translation="MLKPGWNKADDTMYGNFHARRGQEHYHLVVERLPTNNGWDWTVW
                     RPGDAPELSQHGYAPGLLTAMAAAEEVAQYWHARGGTAAAI"
     gene            complement(569..1234)
                     /gene="tenA"
                     /locus_tag="VKQ27_04365"
     CDS             complement(569..1234)
                     /gene="tenA"
                     /locus_tag="VKQ27_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HLY88196.1"
                     /translation="MTEFSTDAWQRTAHLRQMIDMLPFNTELTAGTLSRERFQAYIVQ
                     DALYLAQYSRILAMAGVRGPDAATLRAFGACALEAVAVEQALHERYLAEFGIDPARLA
                     TAEPSPDCLGYTSFLLATAYHEPWEVLVAALLPCFWIYWDVGSRIARTAAAANPYRAW
                     IDTYADEAFGEAVRTVIGITDEAAAATTQAIRQRMMTAFVRSTQYEWLFWDSAYQQRG
                     WPA"
     gene            complement(1231..>2145)
                     /locus_tag="VKQ27_04370"
     CDS             complement(1231..>2145)
                     /locus_tag="VKQ27_04370"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY88197.1"
                     /translation="PREKAEVTVLPEGTVEVIIGTVSQGQGHETTFAQLLTEWLGVPM
                     DSVRLVTGDTDRVSVGGGAHSGRALRLASIVMLNASNEIIAKGLRIAGHILEAAVADM
                     EFADGRFTVKGTDRSIGIFELAAVAARRHDLPDDLRKLDGIGDETINRAAFPYGCHVC
                     EVEIDPETGVVSVANYSAVDDVGRAVNPMIVHGQVHGGIAHGIGQALLEQVWYDPDSG
                     QLLSGSLMDYAIAHADNVPFYQTELSEVPSPTHPLGIRPAGEGGTTPALAAVTNAIVD
                     ALSDYGVTHIEMPATPERIWRAMQGTAT"
BASE COUNT          376 a          669 c          719 g          381 t
ORIGIN      
        1 cggtgggaac ctgccggatg ggggaaggac cggcggctgt cgtcgacgcc aggctgcgcg
       61 tgcatgggat gggcgggcta cgcgtcatcg acgcatcggt catgccgacg ctgaccacgg
      121 gcaatacgaa cgcgccaacg atcatgattg cggagaaggg cgcggcaatg atccgggagg
      181 acgctgcgta acgagtaatt cagatagcgg cggcagtccc gccacgtgcg tgccagtact
      241 gcgccacctc ttcagctgcc gccatcgcag tcaacagccc cggcgcatag ccatgttgcg
      301 aaagctctgg tgcgtcgccc ggtcgccata ctgtccagtc ccagccgtta ttggtgggaa
      361 gtcgctcgac gaccaggtga tagtgctcct ggccacgtcg cgcgtggaaa ttgccgtaca
      421 tggtgtcatc agctttgttc cagcctggct tgagcacgtg agtcatccgg tctgagggcg
      481 gcgcacacga ccacttcaag ccgcctctgg ctgtgatgca gttcacaggt tctatagaaa
      541 ttttgggcgc cgctggtcac aaactggcct atgcgggcca cccgcgctgc tggtaggcgc
      601 tgtcccagaa gagccactcg tactgcgttg agcggacgaa agccgtcatc atccgttgcc
      661 ggatagcctg cgtggttgcg gccgcggcct cgtcggtgat gccgatgaca gtccggacgg
      721 cttcaccgaa agcctcatcg gcataggtgt cgatccaggc gcggtaagga tttgccgcgg
      781 cggccgtcct ggcgatgcgg gagccgacat cccagtagat ccagaagcag ggcagcagcg
      841 ccgcgaccag cacttcccac ggctcatgat aggcggtggc aagcaggaag ctggtgtagc
      901 cgaggcaatc cggcgatggt tcggcggtgg caaggcgcgc cggatcgatg ccgaattctg
      961 caaggtagcg ctcgtgcagc gcctgctcga cggccacagc ctccaatgcg cacgccccga
     1021 atgcgcgcaa tgtggcggcg tcgggtccac ggacgccggc catcgccagg atgcgagaat
     1081 attgcgccag gtaaagtgcg tcctgcacga tataggcctg gaagcgctca cggctgagcg
     1141 tgccggcggt gagttcggta ttgaacggca gcatgtcgat catctggcgc agatgcgcgg
     1201 tgcgctgcca ggcatcggtg gaaaattcgg tcatgtcgcg gttccctgca tcgcccgcca
     1261 gatgcgctca ggtgttgccg gcatctcgat atgggtgacg ccgtagtccg atagcgcatc
     1321 gacgatggcg ttggtgacgg cagcaagggc tggcgtggtt ccgccctcgc ccgcaggccg
     1381 gatgccgagc ggatgcgtgg gcgaaggcac ctcgctgagt tccgtctgat agaacggcac
     1441 gttgtcggcg tgcgcgattg cgtagtccat cagcgagccg gacagcaatt gccctgaatc
     1501 cgggtcgtac cacacctgct ccagcagtgc ctgcccaatc ccatgcgcga tgccgccatg
     1561 cacctggcca tgcacgatca tcggattcac cgcgcgtccc acatcgtcaa cggccgagta
     1621 gtttgccacg ctgacgacgc cggtctcggg atcaatttcc acctcgcaga catggcagcc
     1681 ataagggaag gcggcgcggt ttatcgtctc gtcgccgatg ccgtcgagct tgcgcaagtc
     1741 gtcaggtagg tcatggcggc gtgcggccac cgcggcaagc tcgaagatgc cgatggagcg
     1801 gtccgtcccc ttcacggtga agcggccgtc ggcaaattcc atatcggcga cggcggcttc
     1861 caggatgtgg ccggcgatgc gcaggccctt cgcgatgatc tcgttggagg cgttcagcat
     1921 gacgatgctg gcgaggcgca gcgcgcggcc ggaatgggcg ccgccgccga ccgagacgcg
     1981 gtcggtgtcg ccggtcacca gccgcacgct gtccatcggc acgccgagcc attcggtcaa
     2041 cagctgcgcg aacgtcgtct cgtgtccctg gccctgcgac acggtgccga taatgacctc
     2101 gacggtgccc tccggaagca cggtgacctc ggccttctcg cgcgg
//
LOCUS       DATGEM010000138         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_758564, whole genome shotgun sequence.
ACCESSION   DATGEM010000138 DATGEM010000000
VERSION     DATGEM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758564"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1223
                     /locus_tag="VKQ27_04375"
     CDS             453..1223
                     /locus_tag="VKQ27_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88198.1"
                     /translation="MSRIFISGSSTGLGLMAADLLVGKGHSVVLHARNAERAEAARQE
                     LPGAEAIVIGDVETIAGATHVAAQVNGLGRFDAVIHNAAVGYRESHRVTADGLPHVFA
                     INTLSAYILTALIERPKRLVYLSSGLHHRADANLDDILWKERRWDGSTAYAESKLHDT
                     ILAFAIARRWPDVFSNALEPGWVPTRMGGAGAPDDMDQAHLTQAWLAAGDDPKVDVTG
                     RHFYHLKRVGPNPQADDHALQDRLIAICADISGVALPA"
     gene            1333..1848
                     /locus_tag="VKQ27_04380"
     CDS             1333..1848
                     /locus_tag="VKQ27_04380"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLY88199.1"
                     /translation="MSSRLSADYLQQATPLIHWPQGLEPGNVDVFVHNEGWIDAPPES
                     VWANLIDATQWPSWYSNSADVHIDGDQQKLAKGVTFAWKTYGFPIVSRVDVFELGREI
                     GWSVDSPGFQVHHAWLLIPERGGTRVITEETQKGAAAIKFRLEQPNAMYDGHDWWLSA
                     LKVRSERMAKN"
     gene            complement(1863..>2192)
                     /locus_tag="VKQ27_04385"
     CDS             complement(1863..>2192)
                     /locus_tag="VKQ27_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY88200.1"
                     /translation="VYTPDGGGMSWTFRQGSAETPVTVSGRLKVTAAEGLREAVFAHL
                     GVAITSEWNFAPELASGVVRPVLLDWDLPKLELRAVFPTGRMATAKAREFVSFVESCI
                     PTANYTS"
BASE COUNT          424 a          702 c          667 g          399 t
ORIGIN      
        1 ggcatcggcc tggcctatgc ccgagacatg atcaagccct gatttcgcct ggaattccat
       61 cgggagcggt acgctctcga cccggcgcct gagccttacc gaggaaggtc gcgaccaagg
      121 tgagttgcgc cagaaaggcc gtctctcccg aggcggcttt tttctttccg agcctagctg
      181 cagaacccca tcaaccagga tcctcgccaa gtcgaaaagg ctggtatttc aacgagacgg
      241 cggcgatgcc ggccgccaca ttgatgccct tggcattagg atctgatgat attttgtcat
      301 gaattatctg acttaagggc acttaattca cgagcgttat cgagcgataa ccacccggcc
      361 gcagtcgcaa gcaaccaaca gaatttcgac cctgaccgtt aattgccgtg ccggcggacg
      421 agatccgcca gcgacgcgga aggagcatca tcatgtcacg catcttcatt tcaggatctt
      481 ccaccggcct tggactcatg gcggccgatc tgctcgtcgg caaaggccat agcgtcgtgc
      541 tgcacgcccg caacgccgag cgcgccgaag cggcccgcca ggaactgccc ggggccgagg
      601 cgatcgtgat cggcgacgtc gagaccatcg ccggcgccac gcatgtggca gcccaggtca
      661 atgggcttgg tcgtttcgac gccgtgatcc acaacgccgc ggtcgggtat cgcgaaagcc
      721 atcgcgtcac cgcggatggc ctgccgcatg tgttcgcgat caacaccctg tcggcctata
      781 tcctgaccgc gctgatcgaa cggcccaagc ggctggtcta tctcagctcc gggctgcacc
      841 atcgtgccga cgccaatctc gacgacattc tctggaagga gcgtcgctgg gacggttcga
      901 ccgcttacgc ggagagcaag cttcacgaca cgatcctcgc cttcgcaatt gcgcgacgct
      961 ggccggacgt cttctcgaac gcgctggagc ctgggtgggt gccgaccagg atgggcggcg
     1021 ccggcgcgcc cgacgacatg gatcaggcgc atctgaccca agcctggctt gcggcgggcg
     1081 acgatccgaa ggtcgatgtg acggggcggc atttctatca cctgaagcgc gtggggccga
     1141 acccgcaggc cgatgaccac gcgctgcagg accgcctcat cgcgatctgc gcggacatct
     1201 cgggcgtggc gctgccggcg tgagccggcg cgcgaatctc ctactcaacg actagaggtt
     1261 gctatgaaaa ccaatgcact ctttctgacc gcgagcctta tattgctcgc cgcttcggcc
     1321 gcgcacgggc agatgtccag ccgcttgtcc gccgactacc tccagcaagc gacgccgctg
     1381 atccattggc cgcaaggcct ggagccgggc aatgtcgatg tcttcgtcca taacgaaggc
     1441 tggatcgatg cgccgcccga gagtgtctgg gccaatctga tcgacgccac gcaatggccc
     1501 agctggtact cgaattcggc cgatgtccat atcgatggcg accaacagaa gctcgccaaa
     1561 ggcgtgacct tcgcttggaa gacctacggc tttcccatcg tcagcagggt ggatgtgttc
     1621 gagcttggcc gcgagatcgg ttggagcgtc gatagccccg gcttccaggt ccaccacgcc
     1681 tggctgctga tccccgagcg cggcggtacg cgcgtgatca ccgaagaaac tcagaagggc
     1741 gccgccgcga tcaagtttcg gctcgagcag ccaaacgcga tgtatgacgg ccatgactgg
     1801 tggctgtcgg cattgaaggt gcgttcggag cggatggcca aaaactgacc ggttagtggt
     1861 cactacgacg tgtaattcgc cgttggaatg caggattcga cgaatgacac gaattcccgc
     1921 gccttggcgg tcgccatgcg tccagtcggg aagacggccc ggagttccag cttcggcaga
     1981 tcccagtcga gcaaaacggg tctgaccacc cccgatgcca attcgggagc aaaattccat
     2041 tcggaggtga tcgcgacgcc gagatgcgcg aacaccgctt cccgcaaccc ttcggccgcc
     2101 gtgactttca ggcggccgct gaccgtcacc ggcgtctcgg ctgagccctg gcgaaacgtc
     2161 caggacatcc cgccgccatc cggcgtgtag ac
//
LOCUS       DATGEM010000139         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1224933, whole genome shotgun sequence.
ACCESSION   DATGEM010000139 DATGEM010000000
VERSION     DATGEM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224933"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..648)
                     /locus_tag="VKQ27_04390"
     CDS             complement(151..648)
                     /locus_tag="VKQ27_04390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLY88201.1"
                     /translation="MNAEQLRAMQAPIKQRYRDTPDAARVPARAEARLDAEDVACVVP
                     SWHGETKAGLHPATGGSGELACSADMLLEAVVACAGVTLRAVATAMGVTIRAGRVVAE
                     GTWDARGTLGIDRSAPVGLTDIALKFEVDCDADAAKLERMIQTTERYCVILQTLRNPP
                     RITVG"
     gene            744..1328
                     /locus_tag="VKQ27_04395"
     CDS             744..1328
                     /locus_tag="VKQ27_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLY88202.1"
                     /translation="MPFSLGPEVRLDRPAYLDPTARIYGRVSAGEGSSFWPYSVIRAE
                     GAGVTLGRCCNVQDTAVIHIGGGRGTTIGDYCSIAHRAVVHGADIGDDCLIGIGSVVM
                     DGAKLGRRVIVGAMALVPPGMEVPEGSVVMGVPGKIVATRDNFLPNRRNALVYHRNAL
                     AYAEGRHDAWRADGFAQWRDEMMQRLRAGEEVGM"
     gene            complement(1366..1572)
                     /locus_tag="VKQ27_04400"
     CDS             complement(1366..1572)
                     /locus_tag="VKQ27_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88203.1"
                     /translation="MSREVQVDNEQYYFLIYREPNSNLWRWTLYSPLGRMAAQSVEKY
                     YNKQDCLAAIDAVKASGSAPVRER"
     gene            complement(1569..2243)
                     /locus_tag="VKQ27_04405"
     CDS             complement(1569..2243)
                     /locus_tag="VKQ27_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY88204.1"
                     /translation="MMKLFYTPASPFVRKCTVTLKELGLWDQVEIVLTKWPHSWGTET
                     VPYRTDFLQATPIARIPALVTEDGERLTESSLICEYLNDELGGYRLCPRGGRERWHIL
                     SVVSVVTSGIMECQVARRAEMLRKRDASQNKQEFSSSFVRKMMERMDRCYIRLDELSQ
                     GFGAEVDLGQIAAGCACGLSDFRFPEDDWRGIAPHLARWYERFRQRSSMRETEPGETP
                     FHADET"
     gene            complement(2240..>2605)
                     /locus_tag="VKQ27_04410"
     CDS             complement(2240..>2605)
                     /locus_tag="VKQ27_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88205.1"
                     /translation="ISVDHMGVPRATQGAEEAYRNLPQLLALAKHPNVAVKATGQAGY
                     AQDAYPFRSIHDALHRVFDAFGPRRMFWGTDITRMHCTWRQCVTLFTEELPWLKGADL
                     DLVMGQAWCDWIGWDAARS"
BASE COUNT          458 a          979 c          708 g          460 t
ORIGIN      
        1 atctccatgc gctctactcc agcgacggtg gcgcccgcac aatgccgcgc ctctcatctc
       61 tgcagcaata tgcagcacca gccccccgtg ccaggggacg aaggatccgc aagcgtcacc
      121 ccatccgatg cagcgatcgc atgaggacct tcaccccacc gtaattcgcg gcgggttccg
      181 taacgtctgc aaaatcacgc aataccgctc cgtcgtctgg atcatccgtt ccagcttcgc
      241 cgcgtccgca tcgcagtcca cctcgaactt cagcgcgata tctgtcagcc cgaccggcgc
      301 gctgcgatcg atccccaggg tcccgcgcgc atcccaggtc ccctcggcca ccacgcggcc
      361 cgcgcgtatc gtcactccca tcgccgtcgc gaccgcgcgc agcgtcacgc cggcgcacgc
      421 caccactgcc tccagcagca tgtccgccga gcacgccagt tctcccgatc cacccgtcgc
      481 gggatgcagc cctgccttcg tctcgccgtg ccacgatggc accacgcagg cgacgtcctc
      541 ggcatccagc cgagcttccg cccgtgccgg cacacgcgcc gcatctggcg tatcgcggta
      601 gcgctgcttg atcggcgcct gcatggcgcg aagctgttcg gcgttcatca gcgacccctc
      661 catcatcacg actgcgtttg tcacgcattt gcccggagga caccagcccg gcaccatgtc
      721 cgtcccgcca caggactcct gtcatgccct tttctctcgg ccctgaagtc aggctcgacc
      781 gccccgccta cctcgatccc accgcccgca tctacggtcg tgtatccgcc ggtgagggca
      841 gcagcttctg gccctactcg gtgatccgcg ccgagggcgc cggcgtcacg ctaggccgct
      901 gctgcaacgt ccaggacact gccgtcatcc acatcggcgg cggtcgcggc accacaatcg
      961 gcgactactg ctccatcgcc caccgcgccg tcgtgcacgg cgctgatatc ggcgacgact
     1021 gcctcatcgg catcggctcc gtggtgatgg acggcgccaa actcggccgc cgtgtcatcg
     1081 tcggcgccat ggccctcgtg ccgcccggca tggaggttcc cgagggctcc gtcgtgatgg
     1141 gcgtccccgg caagatagtc gcgacccgcg acaacttcct gcccaaccgc cgcaacgccc
     1201 tcgtctacca ccgcaacgct ctcgcctatg ccgaaggccg ccatgacgcc tggcgcgcag
     1261 acggcttcgc ccagtggcgc gacgaaatga tgcagcgcct gagggccggc gaagaggttg
     1321 gcatgtgatg accgcgttac cgtcatcgat gacggtgacc cggatctatc gctctcgcac
     1381 tggagcggag cccgacgcct tgacggcgtc gatggcagcg aggcagtcct gcttgttgta
     1441 gtacttctcc acgctctgcg ctgccatccg ccccagcgga ctgtacagcg tccagcgcca
     1501 cagattcgag ttcggctccc gatagatcaa aaagtagtac tgctcgttat ccacctgcac
     1561 ttccctactc acgtctcatc cgcgtggaac ggcgtctcac ctggttccgt ctcacgcatc
     1621 gaagaccgct gccggaaccg ctcgtaccag cgcgccaaat gcggggcgat gccacgccag
     1681 tcatcctcag ggaaccggaa atcggaaagc ccgcacgcgc accccgcggc aatctggccc
     1741 agatcgacct cggcgccgaa gccctgcgac agctcatcca gccggatata gcagcggtcc
     1801 attcgctcca tcatctttcg cacgaagctt gacgagaact cctgcttgtt ctgcgacgcg
     1861 tcgcgcttgc gcagcatctc tgctcgtcgc gcgacctggc attccatgat tccgctcgtc
     1921 accacggata cgaccgacag gatatgccac cgctcccggc caccccgcgg gcacagccga
     1981 taaccgccca actcgtcgtt caggtactcg catatcagcg acgattccgt cagccgttcc
     2041 ccatcctcgg tcaccagcgc aggaatgcgc gcaatcggcg tcgcctgcag aaagtccgtg
     2101 cggtacggca cggtttcggt gccccaggaa tgtggccatt tcgtcagaac gatctccacc
     2161 tggtcccaaa gaccaagctc cttcaacgtc accgtgcatt tccgcacgaa cggcgaggcc
     2221 ggtgtataga acagcttcat catgaacgcg ctgcatccca gccgatccag tcacaccacg
     2281 cctgtcccat caccagatcc agatcagctc ctttcagcca gggcaattcc tccgtgaaca
     2341 gcgtgacgca ctggcgccac gtgcagtgca tgcgcgtaat atcggtgccc cagaacatgc
     2401 gccgaggccc gaacgcatcg aacacccgat gcaatgcatc gtggatgctg cggaatggat
     2461 aggcatcctg cgcatagccc gcctggccgg tcgcctttac ggcgacattc gggtgcttcg
     2521 ccagcgccag caattgcggc agattgcgat aggcctcctc cgctccttgc gtcgcacgcg
     2581 gcacgcccat gtgatccacg ctgat
//
LOCUS       DATGEM010000140         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_416428, whole genome shotgun sequence.
ACCESSION   DATGEM010000140 DATGEM010000000
VERSION     DATGEM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416428"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..638)
                     /locus_tag="VKQ27_04415"
     CDS             complement(135..638)
                     /locus_tag="VKQ27_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HLY88206.1"
                     /translation="MKKISIFVTAALTFTQVSAHAFEAVANVVGVTPITEQVNHPTQQ
                     CWTETQQTTQAVPQQRNFLGAIIGGVAGGLLGAQVGRGNGRIAGAAVGAGVGALAGDA
                     VANQNAGPGTVTTTTPVQRCRQVDNFQAVTTGYMVTYEYAGQRFSTRLPYNPGNQLRV
                     NVAVTPR"
     gene            938..2116
                     /locus_tag="VKQ27_04420"
     CDS             938..2116
                     /locus_tag="VKQ27_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88207.1"
                     /translation="MLALVLSTIGGVGMWSVVVALPAVQAEFGVARAAASLAYTLTML
                     GFGAGTVALGRMTDRFGVLPPVVIGTICLTLGYSAAASASTLWQYSIAHGFIGFGSSA
                     TFSPLLADTSHWFARRRGISLAIIASGNYLAGTLWPSVVQHFIETSGWRQTFIGIAIF
                     CAATMLPLVLALRRRAPGHGAATAATHETAMRNRPLGLSPQMLQNLLIVAGVCCCVAM
                     SMPQVHLVAYCGDLGYGAARGAQMLSLLLACGIVSRLVFGWVSDRVGGLRTLLIGSSL
                     QCFALFLFLPFNGLASLYVISALFGLFQGGIVPSYAMIIREYFSPKEAGARIGIVLTA
                     TMLGMALGGWMSGVIFDLTGSYRMAFLNGIAWNLVNMAIALILLGRSRRGQVRAPAGA
                     "
     gene            2152..3180
                     /locus_tag="VKQ27_04425"
     CDS             2152..3180
                     /locus_tag="VKQ27_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY88208.1"
                     /translation="MSASISRRRVLAAGTASAVLISAGIPFRAQAQGAIALRVSSSLT
                     ADQNSAHYIWFQRFAANLKAVAGSRITADFFPNSQLGKEADVVQQVKVGSIDVMLTGS
                     SIWATVAPEFGMLDLGYVFDSYAHMARALDGGVGASLADMLRNRTGCTVMGWGSHFAA
                     RSVYTKAPVTSLAELKGVKLRVLPTSAFIDTFKLMGAVPTPIPFNELYTAVQTGVVEG
                     FEHDASTVLATKLYEVTRHCFLTEHLFPPMVAVIGKRGLDRIPADIRPAFLQAAQEAS
                     LYQRAQAGEKGRAAFAELKKLGITFHPMAKAERESLRKQMEGQLWPAFADQYPPTKPL
                     FKAIAAAR"
     gene            3208..5082
                     /locus_tag="VKQ27_04430"
     CDS             3208..5082
                     /locus_tag="VKQ27_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLY88209.1"
                     /translation="MKHNAAYASQFMRSRAWHRVLNRLLVATEIFAALVLAADVLVVL
                     FSVVWRYFLHDPVDWAEEIARALMGMQVFLGAATTLARVQHVGIDSFRNLFPASWRPV
                     LIQLCHWIIVVVAIALLLSSCALFADSRGQTTPIGLPQWIYVAPVVIGSALMLLFGMA
                     GALDGPGVTVWGTLAVAAMLAGAVCTWNAFLPAHSITPLMLLATGFLLSVVAGLPIAF
                     ALAFSALLYFLADPSLPMLVYSQQVLSGMDHFVLLAIPFFVLAGLLMEANGMSSRLIE
                     LLLRMLGRVRGGLNLIIITATAFFSGVSGSKLADVAAVGGIIMPAVRRTRQDPNDAAG
                     LLAASAVMAETIPPCINMIIMGFVANISIGGLFIAGLVPAAFMALSLALVVFAFGSRI
                     DPDSAFEKRRPLVPLVGGALVGLVMIAMIGKGVTSGVATSTEVSAFAVAYALLVGGLA
                     FRELTLRSVLRLFVRSASMAGGILFIVAAASSVSFALTIQQIPQQLSGFMMELGHRFG
                     ADGFVLASTLLMIAFGAILEGAPALIIFGPLLTPIALQLGVSPLHFGIVMVVAMGLGL
                     FSPPVGLGLFATCSITGTEVKDVVRPMAKYLAVLFVALLVLVFVPAFSLWLPARFGFG
                     "
BASE COUNT          777 a         1583 c         1658 g         1111 t
ORIGIN      
        1 tcggcgtgga cgggacgagg gattcccgcc acgcacgaca gcgcaagcaa ggccgcgaat
       61 gcagcccgta aactcaatga tcgattggat gttacggttc ggaccgacga catggctggc
      121 tcccttggtt cgtcttatcg cggagtgacg gcaacgttca cgcgcaactg gttgcccggg
      181 ttgtaaggca gccgggtcga gaagcgctgg ccagcgtatt cgtaagtgac catgtagccg
      241 gtcgtcaccg cctggaaatt gtccacctgg cggcagcgct gcactggcgt agtagtcgtg
      301 accgtgccgg ggcccgcatt ctggttggcg actgcgtcgc cggcgagggc cccgactcct
      361 gcaccgaccg ctgcccccgc gatccgccca ttgccgcgcc cgacctgggc acccagcaaa
      421 ccgcctgcga cgccgccaat gatggcgccg agaaaattgc gttgctgcgg tacggcctgg
      481 gtggtctgct gcgtttcggt ccagcactgt tgcgtcggat ggttgacctg ttccgtaatg
      541 ggcgttacgc cgacaacgtt ggcaacggcc tcgaacgcat gggcgctcac ttgcgtgaac
      601 gtcagcgctg cggtaacgaa tatcgatatc tttttcatgt gctctccttc ctggactggc
      661 ttgccccgac acggtcgggc ctgctgctct aacgccttag agcccggccg gttgaaaggg
      721 ttttgtaacg tagtgtagaa acaggagggg aagacgctag aggaaagaaa aaaaagagga
      781 aaatttgacc gcttctcgcc cgagggcgtt tcccatttag cgtcttctcc ttcctctggt
      841 atttcaggca caatccctag cggtgaggag cgaacgacaa aataacagcg gcattgcgcg
      901 tggcgtggtg gattcccgat acgcatggat acgactggtg ctggcgctgg tcctttccac
      961 cattggcggc gtaggaatgt ggtcggtggt ggtggccttg ccggcggtgc aggcggagtt
     1021 cggtgttgcg cgcgcggctg cctcgcttgc ctacacgctg acgatgctag gtttcggcgc
     1081 cggcaccgtc gcgctgggcc ggatgacgga tcgtttcggc gtcctgccgc ccgtcgtcat
     1141 cggcactatc tgtctcacgc tcggttactc tgccgccgcg tcggcttcca cgctctggca
     1201 gtactcgatc gcccacggat tcatcggttt cggcagttcg gcgaccttct cgccgctgct
     1261 ggcggatacg tcgcactggt tcgcccgccg gcgtggaatc tccctggcga tcatcgccag
     1321 cggcaactat ctcgccggga cgctgtggcc gtcagtcgtc cagcatttta tcgagacgag
     1381 tggatggcgg cagaccttta tcggcatcgc gattttctgc gcagccacca tgctgccgct
     1441 cgtccttgca ttacgcaggc gggcgcccgg gcacggtgct gcgaccgcgg ccacgcatga
     1501 aacggcaatg cgcaaccgtc cgctcgggct gtcgccgcag atgttgcaga acctgctgat
     1561 cgtagccggc gtatgctgct gtgtcgccat gtccatgccg caggtgcact tggtcgccta
     1621 ctgcggggat ctcggttacg gcgccgcgcg cggggcacaa atgctttcgc tgctgctcgc
     1681 gtgcggtatc gtcagccgac tcgtcttcgg ctgggtttcc gaccgcgtcg gcggattgcg
     1741 cacgctgctg atcggatcga gcctgcagtg ctttgcgctt tttctattcc tgccgttcaa
     1801 cggactcgcg tcgctctatg tcatctcggc cctgttcggc ctgttccagg gaggcatcgt
     1861 gccgtcctat gccatgataa tccgcgagta cttctcgccg aaggaggctg gcgcgcgcat
     1921 cggcatcgtg ctcaccgcga ccatgctggg catggcgctg ggcggctgga tgtccggcgt
     1981 catcttcgat ctcaccggct cgtatcggat ggcgttcctc aacggcatcg cgtggaacct
     2041 ggtgaacatg gcgatcgccc tgatactgct gggcaggagc aggcgcggtc aggtgagggc
     2101 gcctgccggc gcctgacggc agaaaaggca atccatcatg gagaaacgaa catgagcgca
     2161 tcaatatcgc gtcgccgcgt gctcgcagcc ggtacggcgt cggcggtcct gatatcggcg
     2221 ggcattccat ttcgtgcgca agcgcagggt gcgatcgcgt tgcgcgtgtc ctcatcgctg
     2281 accgccgacc agaactccgc gcactacatc tggttccagc gctttgcggc caatctgaaa
     2341 gcggtcgcgg gatcgcggat cacggccgat ttttttccca acagccaact cggcaaggag
     2401 gccgatgtcg tgcagcaggt caaggtcggg tcgatcgatg tcatgctgac cggttcgtcg
     2461 atctgggcca ccgtcgcgcc tgaattcggc atgctggacc tgggctatgt gttcgacagt
     2521 tacgcgcaca tggccagggc gctcgatggc ggcgtcggcg ccagtctcgc cgacatgctg
     2581 cgcaaccgca ccggctgcac ggtcatggga tggggatcgc attttgcggc acgcagcgtc
     2641 tataccaagg cgccggtgac ctcgcttgcg gaactcaagg gcgtgaagct gcgcgtgttg
     2701 ccgaccagcg cgttcatcga tacgttcaag cttatgggcg cggtgccgac gccgattccg
     2761 ttcaatgagc tctacaccgc cgtgcagacc ggcgtcgtcg agggttttga gcacgatgcc
     2821 tcgaccgtgc ttgccaccaa gctctacgag gtgacgcggc actgcttcct gaccgagcat
     2881 ctgttcccgc cgatggtcgc cgtgatcggc aagcgcggac tcgacaggat tcccgcggat
     2941 atccgccccg cgttcctgca ggcggcgcag gaggcgagcc tgtatcagcg cgcccaggcc
     3001 ggggagaagg ggcgcgcagc gttcgcggaa ctcaagaagc tgggcattac cttccatccc
     3061 atggcgaaag ccgagcgcga atcgttgcgc aaacagatgg aaggtcaact ctggcccgcg
     3121 tttgcagacc agtatccgcc caccaaacct ttgttcaagg cgatcgcggc cgcgcgctag
     3181 gccgacaggc gcgtggcggg gcgaaacatg aaacacaacg ccgcctacgc ctcgcagttc
     3241 atgcgctcgc gcgcctggca tcgggtcctg aaccggttgc tggtcgcgac ggaaatcttt
     3301 gcggcgctgg tgctcgccgc cgacgtactg gtggtcctgt tctcggtcgt ctggcgctat
     3361 ttcctgcacg atccggtgga ctgggcggag gaaatcgcgc gcgccctcat gggcatgcag
     3421 gtatttctcg gtgctgcgac gacgctggcg cgcgtgcagc atgtcggcat cgacagcttc
     3481 cgtaatctgt ttcccgcatc ctggcgtccg gttctcatcc agctctgcca ctggatcatc
     3541 gtcgtggtgg cgatcgcatt gttgctgtcc tcctgcgcgt tgttcgccga ttcgcgcggg
     3601 cagaccacgc cgatcgggct accgcaatgg atctatgtcg ccccggtcgt gatcggctcc
     3661 gcgctcatgc tgctgttcgg catggctggc gcgctcgatg gtcccggcgt gaccgtatgg
     3721 ggcacgctgg ccgtggccgc aatgctggcg ggcgcggttt gcacgtggaa tgcattcctg
     3781 cctgcacact cgatcacgcc gctgatgctg ctggccacgg gcttcttgct gagcgtcgtc
     3841 gccggcctgc cgatcgcatt cgcgcttgcg ttttccgcgc tactgtattt tctggcagat
     3901 ccctcgctgc cgatgctggt ttactcgcag caggtgctgt cgggaatgga tcatttcgtg
     3961 ttactggcga ttccgttctt cgtgctggcc gggcttctga tggaagccaa cggcatgtcg
     4021 tcgcggctga tcgaactgtt gttgcgcatg ctgggacgcg tgcgcggtgg gttgaacctg
     4081 atcatcatca ccgcgaccgc attcttctcc ggagtatccg gatcgaagct cgccgacgtc
     4141 gcggcggtgg gcgggatcat catgccggcc gtgcgccgca ctcgccagga cccgaacgat
     4201 gccgcggggt tgctggccgc gtccgcggtg atggcggaaa ccattccgcc ctgcatcaac
     4261 atgatcatca tgggattcgt cgccaacata tccatcggcg gactgttcat tgccgggctg
     4321 gtgccggcgg ctttcatggc gctgtccctg gcgctggtcg tctttgcgtt cggttcgcgc
     4381 atcgatcccg attcggcgtt cgagaagcgc aggcctctgg tgccccttgt gggcggcgcg
     4441 ctggtcggcc tggtcatgat cgcgatgatc ggaaaaggcg tgacgtccgg cgttgcaacc
     4501 tcgacggagg tttccgcctt cgcggtcgcc tatgcgctgc tcgtgggcgg gctggcgttc
     4561 cgcgagttga ccttgcgcag cgtgttgcgc ctgttcgtgc gctcggcttc gatggcaggg
     4621 ggaatacttt tcatcgtcgc tgcggcatcc agcgtgtctt ttgcgctcac catccagcag
     4681 atcccgcagc agctttccgg attcatgatg gaactgggcc atcgtttcgg cgccgacggt
     4741 ttcgtgctcg cctcgaccct gctgatgatt gcattcggcg cgatcctcga aggtgcgccg
     4801 gcgctgatca tattcggtcc gctgctgact ccgatcgcgc tgcaactcgg tgtctcgccc
     4861 ctgcacttcg gcatcgtcat ggtggtggca atgggactgg gattgttttc cccgccggtc
     4921 ggcctgggtt tgttcgcgac ctgttcgatc accggcaccg aggtgaagga cgtggtccgg
     4981 ccgatggcaa aatatctcgc cgtgctgttc gtggcgctcc tggtgctcgt gttcgtgccg
     5041 gcgttctcgc tctggcttcc ggcaagattc ggcttcggct gatagggact agagaatagc
     5101 tctcaccgcg gatgcaaagg acgcggagg
//
LOCUS       DATGEM010000141         9407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_74572, whole genome shotgun sequence.
ACCESSION   DATGEM010000141 DATGEM010000000
VERSION     DATGEM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74572"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VKQ27_04435"
     CDS             <1..1594
                     /locus_tag="VKQ27_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY88210.1"
                     /translation="ISFLHSYINPVHEERARGAIARLLPDLAISISSEVCREIREYER
                     TSTVVANAYVLPMMARYLARMQQGLRQIGANCPLLLMMSSGGICTVETALRFPVRLVE
                     SGPAGGAILARHVAARGGYDRAISFDMGGTTAKITLIDDYAPQQSRHFEIARAYRFAK
                     GSGFPVRIPVIDMVEIGAGGGSIARIDSLRRIVVGPDSAGASPGPACYSRGGTHPTVT
                     DADVVLGRIDPARFAGGKIALDPMRAEDAVAHHISATLAMSPRDTARGIAEIVDETMA
                     SAARVHAVENGKETSGRTLIAFGGAAPLHAVRLAQKLGMHRIVVPPGAGVGSAFGFLG
                     APIAYEVVRTLLVRLDALDEATIGSLFAEMRTEAEAVVRLGVATGELVETRTGFMRYR
                     GQGHEVAVPLPNGPLDPAALRAAFDTTYARVYGRVIPKLEIEAVTWTLSLSEPHELPQ
                     QLAVLPTVSAGAPLGRRSMAETTSGETVDAAIYLRSNLDKGASLRGPAVIIEDGTSTI
                     VPTGYVARISAGDDIVIEESEA"
     gene            1594..3255
                     /locus_tag="VKQ27_04440"
     CDS             1594..3255
                     /locus_tag="VKQ27_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY88211.1"
                     /translation="MRRNVSEIELQIMWNRLLSVVEEQAQTLVRTAFSTSAREAGDIS
                     AGVFDLEGRMLAQAVTGTPGHINTMARSVGHFLDVFPTAQMKDGDVYITNDPWKGTGH
                     LYDLVVVSPTFMDGRIVALFACTTHLVDMGGVGQTPEGRQIYHEGLFIPLLRLARAGE
                     MNEDLLAMIRANVREPVQVIGDVYALIACNEIGSRRLVAMMREFALHDLDMLGEAIIS
                     RSRQGMADAISKLPQGTWTASMRIDGYEGPIDLVGTLTIGKDTIHVDYTGTSGMSSYA
                     INCPLCYTEAYTTFGVNCIVAPHIPNNTGTLDAVRVTAPEGTILSATHPAAVYARSVM
                     GHMLPDVVYGCLDQALPGRVPAEGTSNLWTLNLIAGHGLTGIGRAAGTPFMVNSFHSG
                     GAGARPNQDGLSATPFPSGVRNVPVEATEAITPLVIWRKELRQDSGGAGRQRGGLGQV
                     MEIGSREEAAFGIFARFERVKYPARGRNGGSPGQPGRLSLKSGTELKPKGLAVVPPGD
                     RLVVEMPGGGGMGLPQERGTAAIERDVRLGYVSREAAHRDYKVSG"
     gene            complement(3256..4362)
                     /locus_tag="VKQ27_04445"
     CDS             complement(3256..4362)
                     /locus_tag="VKQ27_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY88212.1"
                     /translation="MKITRIDAFQVRWSPEEKPTQHSAFVRVVTDDGLEGVGEASPMQ
                     GGRASLGIIKHDLAPALIGKNPLNHAVLLDTLLHTHVKLGPEGALTGALAALDIAMWD
                     IKGKLFGQPIHRLLGGAWRTALPFYASIGGNGDRSLDDVLRVVEARMKDKPAAIKVRF
                     DNPRTLLDRDIPGDIAKARAVRKLVGDSFPLAFDANNGLTSGGAIRVGRALEELGYWW
                     FEEPVQHYHVKAMGEVARALDITVSAGEQTYTLAALADLIDAGVRMIQPDIVKMGGIT
                     GLVRCAALAHAHGVELVSHQTQPMIGHTANMHVVAAQLQATKPIEWNDSSTRTHAVFE
                     NPPKPVDGLFQLSDAPGLGLRFNEAELAKRQVEV"
     gene            complement(4493..6220)
                     /locus_tag="VKQ27_04450"
     CDS             complement(4493..6220)
                     /locus_tag="VKQ27_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY88213.1"
                     /translation="MAHETGALAQIQRQIMWNRLISVVEEQAQTMIRTAFSTTVREAG
                     DLSAGIFDLAGRMMAQAVTGTPGHVNSMAESVNHFLRKFPAETMKPGDHYITNDPWLG
                     TGHLHDLTVVTPAFHRGRVVGLFANTAHVIDVGGLGMGPEGRSVFEEGMYIPIVKCFD
                     QGEANETFFDFMRAGSRLPVELEGDIYSLCACNDAGARRLVEMMDEFEMDSLDPLANF
                     IFESSRRATLAEVARIPKGTYLAEICSDGYEEQVTLKAAMTIQDDGIDVDYAGTSGLS
                     SRGINVPPAYCRAYSCFGIKCVVAPEIPNNWASLAPFRMKIPEGCILNAPRPYPVSVR
                     HVIGHLLPDLMMGCLHQAVPERVTAEGAAALWNPPLRGGSAVSGQARGNRSVLPDFEI
                     ITFNSGGTGARPTQDGLNATAFPSGVRTMPVEATENVAPVVFWIKELRADSGGAGRTR
                     GGTGQTMEIGGKGDLEFAINAIFDRVANAPKGREGGLDGATGAVALKSGKRLRTKGFQ
                     TIPDGDRLILKLPGGAGMGDPVLRDSAQVARDVRDGLVSADSARTLYKVVIDADGRVD
                     HAATAELRA"
     gene            complement(6220..8307)
                     /locus_tag="VKQ27_04455"
     CDS             complement(6220..8307)
                     /locus_tag="VKQ27_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY88214.1"
                     /translation="MPSARLAVDIGGTFTDLALEHDGKRTTIKVLTTPAAPEQGVMAG
                     VRDILRLSGVSAGEIAIVIHGTTLATNAVIERKGARTALLATQGFRDVIAMGNESRYD
                     QYDLNIVLPEPLVPRYLRLPVPERLDNEGNVLLPLEMAAVRALVPTLKREGVESIAVG
                     FLHAFINPAHERRVRDLLAEELPDVPVSLSSEVSPEMREWERFSTTVANAYVQPLMAR
                     YLSRLADELRETGVSAPVFMMLSGGGLTTIDTACRFPIRLVESGPAGGAIFSATIARQ
                     CGINNVLSFDMGGTTAKICLIDDGKPQTSRAFEIARVGRFRKGSGLPLRIPVIEMVEI
                     GAGGGSIAHVDIMGRIGVGPESAGADPGPACYGRGGTEPAVTDANLALGRYDPARFAG
                     GTMKLDVAAAHDALASRVGGRLGLNAEMAALGVIEMVDENMANAARVHAIESGKTYEG
                     RTLIAFGGGGPVHACRVAEKVGISHVLVPSGAGVGSAIGFLRAPVGYEVVRSLYQRFS
                     TFDVVAVNTLLADMEAEAAAVVAQGSFGEPVAQRRIAYMRYVGQGHEIPVPLPPRPLG
                     EEDVAAVRAAYDVEYTKAYDRPVPGSDVEIMSYAVLVATIPRDELEMPVAGDDAVFSP
                     GATPARTQLVRDTTTGEVSPWSIFDRTALAPGVHIPGPAIIAEDETSTLVSPGWRATV
                     NGLGYIELTREAA"
     gene            complement(8372..>9407)
                     /locus_tag="VKQ27_04460"
     CDS             complement(8372..>9407)
                     /locus_tag="VKQ27_04460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY88215.1"
                     /translation="TYQYAATTLSAASFRTQFSLPLNVRMSLFGASFLEAMADRVGLV
                     RRAYLVLAGPQGEATLRSNRAMQLAEGAKVALFEGAQIASRFPWLHSADLACATLGLE
                     NEGWFDAYSLLRAVRSDAIGHGARFLHDEGVGIDIAVGRASGVRTASGDRFVADNVVI
                     AAGPASGRVAASIGIPLPVEPRKRTAFMIRAPLDGSAMPLVFDTSGMWIRPEGDGFIC
                     GISPPADNDPHPGDDFNPDLHLLEDPLWPILAHRIPALEQLRLQRAWAGHYDQCLLDH
                     NAVVGPIPDIPNLIIASGFSGHGVQHGPATGRAVSELIRFGEYRTLDLTPLGYQRVRD
                     NQPMWEDVIY"
BASE COUNT         1723 a         3187 c         2821 g         1676 t
ORIGIN      
        1 gatcagcttc ctccactcct acatcaatcc ggtccacgag gagcgcgcgc gcggggcgat
       61 tgcccgcctt ctccctgatc tcgccatcag catctcgtcc gaggtctgcc gcgagatccg
      121 cgagtacgaa cgaacctcca ccgtcgtcgc aaatgcctat gtgctgccga tgatggcccg
      181 ctatctcgcg cgcatgcagc aggggctgcg ccagatcggt gccaactgtc cgctgctgtt
      241 gatgatgtcg tccggcggca tctgcacagt ggaaacggca cttcgcttcc ctgtgcgtct
      301 cgtcgagagc ggccccgcgg gcggcgccat cctcgcgcgc catgtcgcgg cacgcggggg
      361 ctatgatcgc gccatctcct tcgacatggg cggcaccacg gcgaagatca cgctgatcga
      421 cgactatgca ccgcagcaat cgcgtcactt cgagatcgct cgcgcctatc gatttgccaa
      481 aggcagcggt ttcccggtcc gcattcccgt gatcgacatg gtggagatcg gcgcgggcgg
      541 cggctccatc gcgcgtatcg actcgctacg ccggatcgtt gtcggtccgg atagcgccgg
      601 cgccagccca ggccctgcct gctatagccg cggcggcacg catcccacgg ttaccgacgc
      661 cgacgtcgtg ctcggccgca tcgatcccgc ccgtttcgcc ggcggcaaga tcgcactcga
      721 tccgatgcgt gccgaggacg ccgtcgctca tcacattagc gccacgctcg ccatgtctcc
      781 gcgtgatact gcccgcggca tcgccgagat cgtcgacgaa acgatggcca gtgctgcgcg
      841 cgtgcatgcc gtcgaaaacg gcaaggaaac ctcgggtcgc accttgatcg ctttcggtgg
      901 tgccgcgccc ttgcacgcag tgcgcctggc gcaaaaactc ggcatgcacc gcatcgtagt
      961 gccacccgga gccggtgtcg gctccgcgtt cggcttcctt ggcgccccta tcgcctacga
     1021 agtcgtgaga accctgctcg tgcgtctgga cgcgctcgat gaagccacga tcggctctct
     1081 gttcgccgag atgcgcaccg aggcagaagc cgtggtccgc ctcggcgtcg caaccggtga
     1141 gctggtcgag acccgcaccg gcttcatgcg ctaccgaggc cagggccacg aggtcgccgt
     1201 gccgcttccc aacggcccac tcgaccctgc agccctgcga gccgccttcg acacgacgta
     1261 cgcccgtgta tacggccgtg tcatcccaaa actggagatc gaagccgtca cctggacgct
     1321 atcgctgtcc gaaccgcacg agttgccaca gcaacttgcg gtcttgccaa cggtaagcgc
     1381 cggcgccccg ttgggtaggc gctccatggc tgaaacaacc agcggcgaaa cggtagacgc
     1441 cgcgatctat ctccgctcca acctcgacaa aggcgcatcc ctgcgaggcc cagcggtgat
     1501 cattgaagac ggcacctcca ccatcgtgcc cactggctac gtcgcgcgta tcagcgcggg
     1561 tgacgacatc gtgatcgagg agagtgaagc ttgatgcgcc gcaacgtttc cgagatcgag
     1621 ctgcagatca tgtggaaccg cctgctctcc gtggtggagg agcaagcgca gaccctcgtg
     1681 cgaaccgcct tcagcacctc ggcgcgcgag gccggcgata tttcggccgg cgtgttcgat
     1741 ctcgaaggcc ggatgctcgc ccaggccgtc acgggcacgc ccggccacat caacacgatg
     1801 gcccgatcgg tcggccattt cctcgatgtc ttccccaccg cgcagatgaa ggacggagat
     1861 gtctacatca ccaacgatcc gtggaaaggc accggccacc tctacgactt ggtagtggtg
     1921 tcgccgacct tcatggacgg ccgcatcgtc gcattgttcg cttgcacgac gcatctcgtg
     1981 gatatgggcg gcgttggcca aactcccgag gggcgccaaa tctaccacga aggcctcttc
     2041 atccccctgc tgcgtcttgc acgcgccggc gagatgaacg aggacctgct tgccatgatc
     2101 cgcgccaacg tgcgcgagcc ggtgcaggtc atcggcgacg tctacgccct gatcgcttgc
     2161 aacgaaatcg gcagccgccg cctggtcgcc atgatgcgcg aattcgcctt gcacgatctg
     2221 gacatgctcg gcgaagcgat catcagccgc agccgccagg gtatggctga cgcgatcagc
     2281 aagctgccgc aaggcacctg gaccgcatcc atgcggatcg atggctacga agggccgatc
     2341 gatctcgtcg gcaccctcac gatcggcaag gatacgatcc atgtggacta cacaggcacg
     2401 tcgggcatgt cgtcctacgc gatcaactgc ccactctgct atacagaggc atatacgacc
     2461 ttcggcgtga actgcattgt cgcgccccac atacccaaca acaccggcac gcttgacgcg
     2521 gtccgcgtca ccgctcccga aggcacaatt cttagcgcaa cccatcccgc cgctgtctac
     2581 gcgcgctcgg tgatgggtca tatgctgccg gacgtggtgt atggctgcct cgatcaggcg
     2641 ctgcccgggc gagtgccggc cgagggcacg tccaacctgt ggacactgaa tctgattgcc
     2701 ggacacggtc ttaccggcat cggccgcgcc gcggggacgc ctttcatggt gaacagcttt
     2761 cactccggcg gcgccggcgc gcgtccgaac caggacggcc tatcggcaac accctttcca
     2821 tccggcgtgc gcaacgttcc tgttgaagca accgaagcaa tcacgccgtt ggtgatatgg
     2881 cgcaaggaac tccgccagga ttctggcggc gccggccgcc agcgaggcgg cctcggccag
     2941 gtcatggaga tcggctcgcg cgaagaggca gctttcggca tcttcgctcg tttcgaacgc
     3001 gtaaaatatc ccgcccgagg ccgcaacggt ggcagccccg gccagcccgg ccgcctgtcc
     3061 ctgaaatccg gcaccgagct taaaccgaaa ggccttgctg tcgttccgcc tggagatcgt
     3121 cttgtcgtcg agatgccagg tggcggcggc atgggtctac cgcaagagcg cggcactgca
     3181 gccatcgaac gcgatgtccg cctcggctac gtttcgcgtg aagcggcgca tcgcgactat
     3241 aaggtctccg gctgatcaga cctccacctg ccgcttcgcc agttccgcct cattgaaccg
     3301 cagccccagc ccgggggcgt cggagagttg gaacagccca tcaaccggct ttggcgggtt
     3361 ctcgaacacc gcgtgcgtgc gcgtcgaaga gtcgttccac tcgatcggct tggtcgcctg
     3421 caactgagcc gcaacgacat gcatattcgc cgtgtgaccg atcatcggct gcgtctgatg
     3481 tgacaccagt tccacgccat gtgcatgcgc tagagcagcg cagcggacta ggccggttat
     3541 accacccatc ttcacgatat ccggctggat catccgcacg cccgcatcga tcagatccgc
     3601 cagcgccgcc agtgtatacg tctgttcgcc cgccgacacc gttatatcca gagcgcgtgc
     3661 cacctcgccc atcgccttta cgtggtagtg ctgcactggc tcctcgaacc accagtagcc
     3721 cagctcttcc agcgcccgcc ccacacggat cgcgccgccg ctcgtcaatc cgttgttcgc
     3781 atcgaacgct agcgggaagg agtcgcccac aagcttccgc accgcgcgcg ccttggcaat
     3841 gtcaccggga atgtcccgat ccaacaatgt ccgtggattg tcgaaccgga ccttgatcgc
     3901 ggcgggcttg tccttcatcc gtgcctcgac tacgcgcagc acatcgtcca gcgaccgatc
     3961 cccattcccg ccgatggagg catagaaggg cagggctgtc cgccatgccc caccaagcag
     4021 ccgatggatc ggctgcccga acagcttgcc cttgatatcc cacatcgcaa tgtcgagtgc
     4081 ggcgagcgcg ccagtcagcg caccctccgg cccaagcttc acgtgcgtgt gcagcaacgt
     4141 atcgagcaac acggcatgat tcagagggtt cttgccgatc agtgccggcg caaggtcgtg
     4201 cttgatgata cccagcgagg cgcgtcctcc ttgcatcggt gacgcctcgc ccacaccttc
     4261 caacccgtca tcggttacaa cgcgtacgaa ggcgctatgt tgcgttggct tctcttccgg
     4321 tgaccaacgc acctggaacg cgtcaatccg ggttatcttc atcgcgtttc ctctcccgac
     4381 gggtgagggt aatcaacacc gactgatgcc ttgcgacaag ctgccgaggc taaacgggat
     4441 gtgtgtgtca tcgacacacg ttggccggcc gacgtctacc gaacgaggtt gctcaggcgc
     4501 gcagttccgc cgtggctgca tgatccaccc tgccgtcagc atcaatcacg accttgtaga
     4561 gcgtacgcgc actgtctgct gacaccagcc catcgcgcac atcacgtgcg acctgtgcgg
     4621 aatcgcgcaa tacgggatcg cccattccgg cgcctcccgg tagtttcagg attagccggt
     4681 cgccatcagg aatggtctgg aaacccttgg tgcgtagcct cttgccggac ttcagcgcga
     4741 cagccccggt tgctccatcc agcccaccct cacgcccctt tggcgcattg gcgacacggt
     4801 cgaagatcgc gttgatcgcg aactccagat cgcccttgcc gccgatctcc atggtttggc
     4861 cagtgccgcc acgcgtgcgc ccggcgccac cggaatcggc gcgcagttcc ttgatccaga
     4921 acaccaccgg cgcaacgttc tccgttgcct ccaccggcat ggtgcgtacc ccggaaggga
     4981 aagctgtggc gttcagtccg tcctgggtcg gccgcgcgcc ggtgcccccc gaattgaacg
     5041 tgatgatctc gaagtccggc aatacagagc gattgccgcg cgcctggcct gacacagcgg
     5101 acccaccacg caatggggga ttccacaatg cagcggcacc ttcggcggta acgcgttccg
     5161 gcactgcctg atgcaggcag cccatcatca ggtcggggag caagtgtccg atcacgtgcc
     5221 gtaccgaaac cggatatggc cgaggcgcat tcaggataca gccttcgggg atcttcatgc
     5281 ggaacggcgc aaggctggcc cagttgttgg ggatctccgg tgccaccacg cacttgatgc
     5341 cgaaacagga gtaggcgcgg caatacgccg gaggcacgtt gatcccgcgc gacgacaagc
     5401 cggatgttcc cgcgtaatcg acgtcgatgc catcatcctg gatggtcatc gccgccttca
     5461 gcgtgacctg ctcctcgtag ccatcggagc agatttccgc gagataggtg cctttcggaa
     5521 tacgagcgac ctctgccagc gtcgcgcggc gcgagctttc gaagatgaag ttggccagtg
     5581 ggtccaggct gtccatttcg aactcgtcca tcatctcgac cagacgccga gcacccgcat
     5641 cgttgcaggc acacagggag tagatgtccc cctccagttc gacgggcagc cgcgaaccgg
     5701 cgcgcatgaa gtcgaagaac gtctcatttg cttcgccctg gtcgaagcat ttgacgatcg
     5761 gtatatacat gccctcttcg aaaaccgaac gtccttccgg tcccatgccg agacccccga
     5821 catcgattac gtgtgcggtg ttggcgaaca gcccgacgac gcggccacga tgaaacgctg
     5881 gggtcacaac agtcaggtcg tgcagatgac cggtgccgag ccaggggtcg ttggtaatgt
     5941 agtgatcgcc cggcttcatc gtctcggcgg ggaacttgcg caggaagtgg ttgacgcttt
     6001 ccgccattga gttgacgtga ccgggcgtgc cggtcaccgc ctgcgccatc atgcgcccag
     6061 ccaggtcgaa gatccccgcg gaaagatcgc ccgcttcacg cacggtggtg gagaaggcgg
     6121 tgcggatcat cgtttgcgcc tgttcctcca ccacagagat cagacggttc cacatgatct
     6181 gtcgctggat ctgcgccagc gcgccggtct cgtgagccat caggcagcct cccgcgtcag
     6241 ttcgatgtag cccagcccgt tcaccgttgc acgccagccc ggactgacca gcgttgaggt
     6301 ctcgtcttcc gcaatgatag ctggcccagg gatgtgtacg cccggcgcca gcgcggtccg
     6361 atcaaagatg gaccacggcg acacttcccc cgttgtcgtg tcgcgcacca gttgcgtgcg
     6421 tgcaggtgtt gcacccggag agaatacggc atcatccccg gcaaccggca tctccagttc
     6481 gtcccgaggt atcgtcgcga cgagcaccgc gtagctcatg atctcaacat cggagccagg
     6541 caccggccgg tcatacgcct tggtatattc aacgtcatag gcagcgcgga cagcggccac
     6601 atcttcctcg ccaagcggcc gaggaggcag cggcaccggt atttcgtgcc cttggccgac
     6661 atagcgcatg tacgcgatgc ggcgttgagc gaccggctca ccgaaactgc cttgcgcgac
     6721 aacggccgct gcttcggcct ccatgtcagc cagcagtgtg ttcaccgcga cgacgtcgaa
     6781 agtggaaaaa cgctgataca ggctgcgaac aacctcatag cccaccggtg cgcgcaggaa
     6841 gccgatggcg ctgccgacgc cggcacccga cggcaccagc acatggctga tgccgacttt
     6901 ctcggcaaca cggcaggcat gcaccggtcc accgccaccg aatgcgatca gcgtgcggcc
     6961 ctcataggtc ttgccggact cgatggcatg gacccgtgcc gcattggcca tgttctcatc
     7021 gaccatctcg atcacgccga gcgccgccat ctcggcattt agccccaggc gcccaccgac
     7081 gcgcgacgcc agcgcatcgt gcgccgcggc aacatcgagc ttcatcgtgc caccagcgaa
     7141 ccgcgccgga tcgtagcgcc cgagcgccag gttcgcatcg gtcaccgccg gttcggtgcc
     7201 gccacgtcca tagcaggcag ggccaggatc agcaccggcg ctctccggcc caacaccgat
     7261 acgccccatg atgtcgacat gcgcaatgga gccgccgccc gcgccgatct ccaccatctc
     7321 gatcaccggg atccgcaacg gcaggccgga ccctttacga aaccgaccaa cccgcgcgat
     7381 ctcgaaggcg cgtgaagtct gtggcttccc gtcatcgatc aggcagattt tcgccgtggt
     7441 gccgcccatg tcgaacgaca gcacattgtt gatgccgcat tgccgggcga tcgtcgccga
     7501 aaagatcgcg ccacccgcgg ggccgctttc caccagccgg atcgggaagc ggcaggcggt
     7561 atcgatggtg gtaagcccgc ccccggacag catcatgaat accggcgcag acacgccagt
     7621 ctcacggagt tcatcggcga ggcggctcag atagcgcgcc atcaacggct ggacataggc
     7681 attggcgaca gtcgtggaga agcgctccca ctctcgcatt tcgggtgaca cttcgctcga
     7741 aagcgacacc ggaacgtccg gcagctcttc tgcgaggagg tcacgcaccc ggcgctcatg
     7801 cgcgggattg atgaacgcgt gcaggaaccc aacagcgatg ctctccacgc cttcgcgctt
     7861 caacgtcggc accagcgcgc gcactgcggc catttcgagc ggcagcagca cattgccttc
     7921 gttgtccagc cgttccggca ccggcagccg cagatatcgc ggcaccagcg gctccggcag
     7981 cacgatgttg agatcgtatt gatcgtagcg actctcgttg cccatcgcga tcacgtcgcg
     8041 gaagccctgc gtggccagca gtgcggtgcg tgcgcccttt cgctcgatca ctgcgttggt
     8101 ggcaagtgtg gtaccgtgga tcacgatggc gatttcgccc gcggacacgc cggacagccg
     8161 caggatgtcg cgtacgcccg ccatcacgcc ttgttccggc gcggcgggtg tggtcaggac
     8221 cttgatagtg gtgcgtttgc cgtcatgctc gagtgcaaga tcggtgaagg tgccgccgat
     8281 atcgacggca agtcgagcag aaggcattag tcctccgggt tgtctcagag tgcggcagtg
     8341 aaccttggcc gcggcgtgct ggcaagcggg gtcagtaaat tacatcttcc cacatcggtt
     8401 ggttgtcgcg cacccgctgg tagcctagtg gcgtgaggtc cagggtccgg tactcgccga
     8461 atcgtatcag ctcggaaaca gcgcgccctg tcgcagggcc gtgttgcact ccgtgcccgg
     8521 aaaagccaga cgcaatgatc aggttgggga tatcggggat gggcccgacc accgcgttat
     8581 ggtccagcag acactggtca taatgcccgg cccaggctcg ttgcaaacgc aactgttcca
     8641 gcgccggaat tcggtgcgcc aggatcggcc acagcggatc ctccagcagg tgcaggtccg
     8701 gattgaagtc atcccctgga tgagggtcgt tatccgccgg cggcgatatg ccacagataa
     8761 atccgtcgcc ttcgggcctg atccacatgc ccgatgtatc gaacaccagc ggcattgcgc
     8821 tgccatcgag cggcgcgcgg atcatgaacg ctgtgcgctt gcgcggctcc accggcaatg
     8881 gaatgccgat gctcgccgcg acgcggccag atgccggtcc agccgcgatc acgacgttat
     8941 ctgccacgaa gcggtcgccg gatgcggtgc gcacgccgct agcccggccc acggcgatgt
     9001 cgatcccaac accctcgtcg tgcaggaacc tggcgccgtg cccgatcgcg tcggagcgca
     9061 ccgcacgcag cagactgtaa gcatcgaacc agccttcgtt ctccagcccc agcgtggcac
     9121 aggccaggtc ggcagagtgc aaccagggga accgcgatgc gatctgcgca ccctcgaaca
     9181 acgcgacctt ggcaccctcg gcaagctgca ttgcacggtt ggagcgcagc gtcgcctcgc
     9241 cttgcggccc ggccagcacg aggtaggctc ggcgcaccag tccgacacgg tccgccatcg
     9301 cctccaggaa cgatgcgccg aacagggaca tgcgcacgtt tagtggcagc gaaaactggg
     9361 tacggaatga cgctgcggac agcgttgtgg cagcatattg gtaggtc
//
LOCUS       DATGEM010000142         6605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_304456, whole genome shotgun sequence.
ACCESSION   DATGEM010000142 DATGEM010000000
VERSION     DATGEM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304456"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1012)
                     /locus_tag="VKQ27_04465"
     CDS             complement(32..1012)
                     /locus_tag="VKQ27_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLY88216.1"
                     /translation="MASFTTTAAPEARGQRLDRFLADAIGTLSRSRVKTLIEQGQARR
                     DGKVLNEPSELVRPGAIYELALPPPAPATPQPQSIPFPILYEDADLIVLDKPAGLVVH
                     PAPGNEDGTLVNALLAHCGDQFTGIGDERRPGIVHRLDKDTSGLMVVAKTQLANNALT
                     AAFAARDLDRAYLALVWGLPSPLEGDIEGDIGRDKRDRKRMAVVTRNGKPALTHYRVL
                     RAWASSVSLLECRLATGRTHQIRVHLASRGHPVVGDPLYLRRVPAVARSLPEPIRHPL
                     LDFPRQALHAARLGFVHPRTGEPLEFSTEVPADMAEILSSLDRDLRVAQR"
     gene            1024..1311
                     /locus_tag="VKQ27_04470"
     CDS             1024..1311
                     /locus_tag="VKQ27_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6476 family protein"
                     /protein_id="HLY88217.1"
                     /translation="MRFLKALVIGMGVLIVVATTVLIVLIVHRLSGPRALPPVAAGPA
                     AVDEPTGTRIVTIVAVGDRLAVQLQGGGPDRVLLVDPQTGSVVGRLALRVP"
     gene            complement(1317..1883)
                     /locus_tag="VKQ27_04475"
     CDS             complement(1317..1883)
                     /locus_tag="VKQ27_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLY88218.1"
                     /translation="MPEARSRGYFGIGVEGVSKSANVGALLRTAHAFGAAFCFVVGAG
                     FDARAGRLADTADTPDHVPLWRFDTLDTLALPQRCVLVGVELLDNATDLPSFRHPLNA
                     AYVLGPERAGLSPAMLQRCQHVVRIPTRFALNLAVAGALVLYDRLLQHGRFGERPVGS
                     RSEATDVPPRSGHGSPRFRRNIPDWVGC"
     gene            1882..3153
                     /locus_tag="VKQ27_04480"
     CDS             1882..3153
                     /locus_tag="VKQ27_04480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY88219.1"
                     /translation="MAAVYAAVMTDATDARLAAQYEAFPYPRRDPRDEAKRLIVGSPG
                     HLREIDYWIFGARRPVTQPLRALIAGGGTGDATIMLAQQMARGGRSGSVTWLDRSAAS
                     LKIAQARAAARGLTNIIWEQRSLLDLPGSGLGPFEYIDCCGVLHHLPDPAEGLRALLS
                     VLAPGGGLGLMVYAPHGRTGVYMLQEALRLLAPADERPDARLDVVKRVMRHLPETAWL
                     RRNNFLDDHVNGGDAGLYDLLLNPRDRAFSVRDLHAMLMGEGMEVSCWVEPTRYDPIP
                     LLPDPRLRTRVEAMQPLDRAALAEALAGNVSAHIVYCMRSAEMAPRADFTAPDAVPVA
                     REIPSPELANGIQPDGTITVLFDGLRLPLALPPLAPAILRLVDGRRCVAEIAASLAER
                     GTGAEAFERAWRATFVPLERINRLLLAAPLS"
     gene            3258..6008
                     /locus_tag="VKQ27_04485"
     CDS             3258..6008
                     /locus_tag="VKQ27_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HLY88220.1"
                     /translation="MVDTSPTVADEVKTTTCYMCACRCGIRVHLKDGRIRYIDGNPRH
                     PVNRGVICGKGSAGIMNHYSPARLRKPLLRTGERGSGDFKEIEWDEALALATEWLSAI
                     RATEPRKLAFFTGRDQSQSFTGWWAKQFGTPNFAAHGGFCSVNMAAGGLYTVGGSFWE
                     FGEPDWERTKYLLMFGVAEDHDSNPIKMGIAKLKARGAKFVSVNPIKTGYSAVADEWV
                     GIKPGTDGLFVLSLVHELLAADRIDLEFLARYTNAGWLVGSDGLFVRDGDGKPVLAER
                     AAGEVTLADGRRAYPVFHLLAERYLDGRYSPEVAAETCGVPAETIRRIARELADVAFD
                     QTITLDQPWTDTSGRRHETMLGRPVAMHAMRGISAHSNGFHTCRSIHLLQMLLGAVDT
                     PGSWRYKSPYPRPIPAGPQPVGKGAKPNSMLPGMALGNPRGPEDLLLEDDGSPVRIDK
                     AFTWDAPMAAHGLMHTLIANAHAGDPYKIDTLFMYMANMAWNSSMNPGDASRMLADRD
                     PATGQYRIPHVIYSDAFASETIAYADLVLPDTTYLERWDCISLLDRPIASPEGPADAI
                     RQPVMKPDRDVRPFQDVLLELGMRLKLPGFVKDDGSAMYGSYADYIARHERRPGVGPL
                     AGFRGEDGNAAGVGPANPDQLQRYVENGCFWSYHHAPEALYYKHANVAYLEDAKRLGL
                     IDHATQIVMQIYSEPLQKFRLAAEGQGPNQPPDAQRERVRKYFDPLPIWYPPFEQSLP
                     EQAAYPLHAVTQRPMAMYHAWHSQNAWLRQIYSHNRLYIHRRTAAALGIADDDWVWVI
                     SRHGRVKGQVRLMEGVNPDTVWTWNAIGKRRGAWGLADDAPEAERGFLLNQVISEFLA
                     GGRELNADPVTGQAAWYDLRVRVEKAEGPAETAPLAAALHHPPGLPRAPRTLQYDASE
                     AS"
     gene            6005..>6605
                     /locus_tag="VKQ27_04490"
     CDS             6005..>6605
                     /locus_tag="VKQ27_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLY88221.1"
                     /translation="MTDIPAPGAKKLGLVIDLDTCVGCHACAVNCKEWNTSGYSAPLT
                     DFNAYQDHPDGIWLNRVHTYEAGEGADSRTVHFPRSCLHCEQPACVTVCPTGASYKRA
                     EDGIVLVNADICIGCKLCSWACPYGAREFDEDQGVMRKCTLCIDRIYNEHIPEHERVP
                     ACVAACPAKARHFGDLGDPRSAVSQLVAERGGFDLMPEMG"
BASE COUNT         1139 a         1971 c         2316 g         1179 t
ORIGIN      
        1 ggattagtcc tgtcaaggat tctgccctag gtcagcgttg cgcgacgcgc agatcacggt
       61 ccagtgagga caggatttcc gccatatctg ccgggacttc ggtgctgaac tcgagaggct
      121 cgccagtgcg tgggtggacg aaaccgagcc gcgccgcatg cagtgcctgg cgaggaaagt
      181 ccagcagtgg gtgacggatc ggttcaggga gcgagcgagc gacagcgggc acgcgccgca
      241 ggtaaagagg gtcgcccacg acagggtgcc cacggctggc cagatggacg cgaatctgat
      301 gggttcgacc ggtcgccagg cggcattcga gcagcgacac ggagctagcc caggctcgga
      361 gcacacggta gtgggtcagt gctggcttgc cgttgcgggt caccacggcc atgcgcttgc
      421 ggtcgcgctt gtcacggccg atgtcgccct cgatatcgcc ctccagtggc gaaggcaggc
      481 cccagacgag cgccagataa gcgcggtcca ggtcgcgggc cgcgaaggcg gcagtcagcg
      541 cattgttggc gagctgggtc ttagcgacga ccatgaggcc ggacgtgtct ttgtccaacc
      601 gatggacgat gccggggcgg cgctcgtcgc caatgccggt gaactgatcg ccacaatggg
      661 caagcagggc gttgaccagt gtgccgtcct cgttgcctgg ggcggggtgg accacgaggc
      721 ctgctggttt gtccaaaacg atcagatcgg cgtcctcgta aaggatcggg aatgggatgg
      781 actgcggctg cggtgtagcc ggcgccggcg gcggcagtgc cagctcatag atcgcccctg
      841 gccgcaccag ttcggacggc tcattgagaa ccttgccgtc gcggcgtgcc tggccctgct
      901 cgatcagcgt cttgacgcgg gagcgggaga gcgtgccgat ggcgtcggcc aggaagcgat
      961 cgagccgctg cccgcgcgcc tctggcgccg ccgtggtggt gaacgaggcc atggaggaga
     1021 agtatgcggt tcctgaaggc gctcgtcatt ggcatgggcg tgctgatcgt ggtggcgacc
     1081 acggtgctga tcgtgctgat cgtgcaccgg ctgagcggtc cgcgcgccct gccgcctgtc
     1141 gctgcggggc cggcggccgt ggacgagccg accgggaccc ggatcgtgac gatcgtagcg
     1201 gtcggcgacc ggctggccgt gcagttgcag ggcggtgggc cagatcgggt cctgctggtt
     1261 gatccacaga ccgggtcggt cgtcggccgg cttgcactgc gggtacctta gcccgatcag
     1321 cagccaaccc aatcagggat attgcgtcgg aagcgcgggc tgccatgacc cgatcgcggt
     1381 ggaacgtcgg tggcttcgct gcggcttcct accggacgct cgccaaagcg gccatgctgg
     1441 agaagccgat cgtaaagcac cagcgcacca gctaccgcta gattgagggc gaatcgtgtt
     1501 gggatccgca ctacgtgctg gcagcgctgc aacatcgccg gtgaaagccc ggcacgctcc
     1561 ggtccaagca cgtacgccgc gttcagcggg tgccggaacg acggcaggtc ggtggcgttg
     1621 tcgagcagtt ccactccgac cagcacgcag cgctgtggca gcgccagggt atcgagggtg
     1681 tcgaagcgcc atagcggcac gtggtccggc gtgtcagcgg tatcggccaa cctcccggcg
     1741 cgcgcatcga agccagcgcc aaccacgaag cagaatgccg cgccgaatgc gtgcgcagtg
     1801 cgcaacagag cgccgacatt ggccgacttc gacacgccct cgacgccgat accgaaatag
     1861 ccccgcgatc tagcctctgg catggcagcg gtctatgccg cggtcatgac cgacgcaacc
     1921 gatgccaggc ttgctgcaca atatgaagcg ttcccttatc cccgccgcga tccgcgggac
     1981 gaggcgaaac ggctcatcgt tggtagtccc ggccatctgc gcgagatcga ttattggata
     2041 ttcggcgccc ggcgccctgt gacgcagccg ctgcgggcgc tgatcgccgg cggcggaacc
     2101 ggcgacgcca ctatcatgct ggcgcagcag atggcgcgcg gtgggcgatc cggcagtgtg
     2161 acctggctcg accggtcagc ggcgtcgctg aagattgcgc aggcgagggc ggcggcccgc
     2221 gggctgacga acattatctg ggagcagcgc tcgctgttgg atctgccagg ctccggtctt
     2281 ggtccgttcg aatacatcga ttgctgcggt gtcctgcatc atctgccgga tccggccgag
     2341 ggactgcgcg ctctgctctc cgtgctggcg cctggcggcg gtctcggact gatggtgtac
     2401 gcgccgcatg gccgcacggg cgtgtatatg ctgcaggagg cgttgcgcct gttggcgccg
     2461 gccgacgagc gaccggatgc gcggctggat gttgtcaagc gcgtgatgcg gcatctgccg
     2521 gagacggcgt ggctgcggcg gaacaacttc ctcgacgacc acgtgaatgg cggcgatgcc
     2581 ggcctgtacg acctgctgct gaacccgcga gatcgtgcgt tcagcgtgcg cgacctgcac
     2641 gcaatgctaa tgggagaagg gatggaggtg agttgctggg tggagccgac ccgctacgat
     2701 ccgatcccat tgctgccaga tcctcgtctt cgcacccggg tcgaagcgat gcagccactt
     2761 gatcgcgcgg cgctcgctga ggcactggcc ggcaatgtca gcgcccatat cgtctattgc
     2821 atgcgctccg cggaaatggc gccgcgtgcc gacttcaccg caccggatgc cgtgccggta
     2881 gcgcgggaaa ttcctagccc cgaactcgcc aacggcatcc agccggatgg tacgatcacg
     2941 gtattgttcg atggcctgcg gttgccgctt gcgttgccac cgctcgctcc ggcgatcctg
     3001 agattggtcg atggtcgccg ctgcgtagcc gagatcgccg cctcgctggc agagcgcggg
     3061 actggcgccg aggcattcga acgagcctgg cgagcgacct tcgtaccact ggaaaggatc
     3121 aatcggctgt tgttagccgc gccgctctct tgatcctggg gctacggggt gctttttgct
     3181 cccgacggat cgggagggag ttggatttgg catacgcgaa agcctcccgg acacgcggtt
     3241 tcgcgcgtcc ggttgagatg gttgatacgt cccctaccgt tgccgatgag gtaaagacca
     3301 ccacttgcta catgtgcgct tgccgctgcg gcatccgcgt gcatctgaaa gacggccgca
     3361 tccgctacat cgacggcaat ccgcgccatc cggtgaaccg cggcgtcata tgcggcaagg
     3421 gttccgccgg tatcatgaac cactactcgc cggcacggct gcgcaagccg ctgctgcgca
     3481 ccggcgagcg cggcagcggc gacttcaagg agatcgagtg ggacgaggca ctggcactcg
     3541 ccactgaatg gctaagcgcg atccgcgcga ccgaaccgcg taagctggcg ttcttcaccg
     3601 ggcgcgatca gagccagtcg ttcaccggat ggtgggccaa acagttcggc acgccgaatt
     3661 tcgcggcgca tggcgggttc tgttcggtga acatggctgc cggcgggctg tatacggtcg
     3721 gtggcagttt ctgggaattc ggcgagccgg attgggagcg gaccaagtat ctgctgatgt
     3781 tcggcgttgc cgaggaccac gattccaacc ctatcaagat gggcatcgcg aagctgaagg
     3841 cgcgcggggc gaaatttgtt tcggtcaatc cgatcaagac tgggtactcc gcggttgccg
     3901 acgaatgggt tggcatcaag cctggcacag atgggttgtt cgtgctgtca ctcgtgcacg
     3961 aactgctggc ggcggatcgc atcgacctgg agttcctggc acggtacacg aatgcaggct
     4021 ggctggtcgg gtcagatggg ttgtttgtgc gcgatggcga cggcaagcca gtgctggcgg
     4081 agcgcgcggc cggcgaggtg acgctggcag atgggcggcg tgcatatcct gtgttccacc
     4141 tgctggcaga acgttacctc gacggacgtt acagccctga ggtcgcggcg gagacctgcg
     4201 gggtgccagc cgagacgatt cggcgcattg cacgcgaatt ggcggatgtg gctttcgacc
     4261 agacgatcac gctggaccag ccatggaccg atacgtcggg acggcggcac gagacgatgc
     4321 tcggccggcc ggtagcgatg catgcgatgc gcgggatttc cgcgcactcg aacgggttcc
     4381 atacctgccg ctcgatccat ctgctgcaga tgctgctggg ggcggtcgat acgccggggt
     4441 catggcgcta caagtcgcca taccctcggc cgatcccggc gggaccgcag ccggtcggca
     4501 aaggcgccaa accgaattcg atgctgccgg gcatggcgct cggcaatccg cgcgggcctg
     4561 aggatctgct gctggaggat gatggcagcc cggtgcgtat agacaaagcg tttacctggg
     4621 acgctccgat ggctgcgcat gggctgatgc atacacttat cgccaacgcg catgccggcg
     4681 acccgtacaa gatagacacg ctgttcatgt acatggcgaa catggcgtgg aactcgtcga
     4741 tgaatccggg cgacgcgtcg cgcatgctgg ctgaccgtga tccggcgacg ggtcagtatc
     4801 ggataccgca tgtgatctac tcggatgcgt ttgcctcgga gacgatcgcc tacgcggatc
     4861 tggtgctgcc tgacacgacg tatctcgagc gctgggattg catttctttg cttgatcgtc
     4921 cgattgcttc cccggaggga ccggcggatg cgatccgtca gccggtgatg aagccggatc
     4981 gggacgtgcg gccgttccag gacgtgctgc tcgagcttgg catgcggttg aagctgcctg
     5041 gtttcgtcaa ggacgatggc agcgcgatgt acggcagcta tgccgactac attgcgcgtc
     5101 atgagaggcg cccgggtgtc ggaccgctcg ctggattccg cggtgaggat ggcaacgcgg
     5161 ccggggtggg cccggcgaat cccgatcagt tgcagcgata tgtcgagaat ggctgcttct
     5221 ggtcgtacca ccatgcgccg gaggcgctgt attacaagca cgctaatgtc gcgtatctgg
     5281 aagatgccaa gcggctcggt ttgatcgacc atgcgacgca gatcgtgatg cagatctaca
     5341 gcgagccatt gcagaaattc cggctcgcag cggaagggca aggtccgaac caaccgccgg
     5401 atgcgcagcg cgagcgggtg cggaagtatt tcgatccgct gccgatctgg tatccaccat
     5461 tcgagcagtc cctgcccgag caggcggcgt acccgctgca cgcggtgacg cagcggccga
     5521 tggcgatgta ccacgcgtgg cattcgcaga atgcgtggct gcggcaaatc tatagccaca
     5581 atcgtctcta catccatcgc cggacggcag cggcgctcgg cattgccgat gacgactggg
     5641 tgtgggtcat aagccgccat ggccgcgtaa aaggacaggt gcggctgatg gagggggtca
     5701 acccggacac ggtctggacc tggaacgcga tcgggaagcg ccgtggcgcc tgggggctgg
     5761 ccgacgatgc ccctgaggcg gaacgggggt tcctgctgaa tcaggtgatc tcggagttcc
     5821 ttgccggtgg gcgggaactg aacgccgatc cggtgacggg gcaggcggca tggtacgatt
     5881 tgcgggtgcg ggtcgagaag gcggaagggc cggcagagac ggcgccgctg gcagcggcat
     5941 tgcatcatcc gccagggctg ccacgggcgc cgcgaacgct gcaatatgac gcgtcggagg
     6001 cgtcatgacc gacattccgg cgccgggggc caagaagctc ggactggtca tcgacctgga
     6061 cacctgtgtc ggctgtcatg cctgcgcggt gaactgcaag gagtggaaca cgtcgggcta
     6121 ttccgcgccg ctcaccgact ttaacgccta tcaggatcat cctgacggca tttggctgaa
     6181 ccgggtgcat acctatgagg caggtgaggg tgcggacagc cggacagtgc attttcctcg
     6241 gtcctgcctg cactgcgagc aaccggcctg cgtgaccgtc tgccctaccg gcgccagcta
     6301 caagcgcgcg gaagacggca tcgtgctggt gaacgccgac atctgtatcg gctgcaagct
     6361 atgctcctgg gcatgcccat atggtgcgcg ggagttcgat gaggatcagg gcgtgatgcg
     6421 caagtgcacg ctctgtatcg accgtatcta caacgagcac attcccgagc atgagcgcgt
     6481 gccggcctgt gtcgcggcgt gcccggcgaa ggcgcggcat ttcggcgacc tcggcgatcc
     6541 gcgcagtgcg gtgtcgcaac tggtggcgga gcgcggcggc ttcgatctga tgccggaaat
     6601 gggct
//
LOCUS       DATGEM010000143         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_304369, whole genome shotgun sequence.
ACCESSION   DATGEM010000143 DATGEM010000000
VERSION     DATGEM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304369"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1089
                     /gene="iolE"
                     /locus_tag="VKQ27_04495"
     CDS             109..1089
                     /gene="iolE"
                     /locus_tag="VKQ27_04495"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HLY88222.1"
                     /translation="MAIRFGVSPIAWINDDMPELGGDTPVETVLADARDLGFTGIELG
                     GKFSKDPAVLEPLLAAHGLGLVGGWYSGNLLSHGARDEIASLQSHLALLKAMRCTVFV
                     HAETSNAVHGDRARPLAATPQLDAGAWAQFGERLTEVADYIASQGLRYAYHHHLGTVV
                     DGLDDLENFMRYTGATVGLTVDTGHAALAGVDPVELIRSFPERVAHVHCKDIRGEVFR
                     DVMLRHGSFLEGVLAGMFTVPGDGDLDYDAVLRALAGIGYGGWIVVEAEQDPAKANPR
                     RYARVGLRTLRQAAAAAGLAEAGLHVCEPCDAREPRDACGPRDACEPRDE"
     gene            1046..1888
                     /gene="iolB"
                     /locus_tag="VKQ27_04500"
     CDS             1046..1888
                     /gene="iolB"
                     /locus_tag="VKQ27_04500"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HLY88223.1"
                     /translation="MHAGRAMHASRAMSKLLVRSREPDENGCILEVTPASAGWSRVGF
                     KVCRLTVGQRIEGFSADRETCIVLLKGNAAIRTADRDFGSIASRASVFDDAAPGAVYV
                     PAGIRYGVAARTLAEVAICSAPGGRGGESRLIAASKMLCEVRGQGTNTRYVRNILPQT
                     APADSLLVVEVITPGGHWSSYPPHKHDSGADGEETALEETYYHRLDPPQGFAVQRVYT
                     EDGSLDETLCVQDGDVVLVPRGYHPVGAAHGYTLYYLNVMAGPERKWIARNEPRHDWI
                     ARKK"
     gene            2008..3390
                     /locus_tag="VKQ27_04505"
     CDS             2008..3390
                     /locus_tag="VKQ27_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HLY88224.1"
                     /translation="MLIAFIALTARIMLTVLALASVAAAAGNFAEVTPSPQQVSWQDL
                     EFGVLVHFGTNTYLDREWGDGTAPPAVFNPDKFDPEQWVLAAQAAGAQYLVMVAKHHD
                     GFCLWPTEESDYSIKASPWKNGHGDMVAEVAAAARKHGMKFGVYLSPWDRHEPKYADA
                     AAYDEHYQAQLRELAGNYGELAEFWLDGAGSQGHVYNFDRYVETLRIYQPNTLIFADV
                     ALLDYGDARWAGNEDGVVNGEDWNVVDRHGLLRWRPVEADTPLRKNWFWHASDAAKGV
                     EKLVQIYETSIGRGGQLMLGLSPDMHGLLSAGDVARLREFGAAIRALYGDNLMRKRHA
                     VDDPELERALDDDRETFWSAPAGSHHATIEVRFDKPVTFDRAMTMEWLEGGQRVHAYR
                     VEVWTGAGWKTVAQAQAIGHKKIDIFDPITADRVRLNLLSTTDAAQIREFALYDSRAL
                     HISRGTKRLP"
     gene            complement(3374..>4169)
                     /locus_tag="VKQ27_04510"
     CDS             complement(3374..>4169)
                     /locus_tag="VKQ27_04510"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88225.1"
                     /translation="LRHARIHDQLASNPQYAGGIAWCAFDYNTHGYFGSGDRICYHGV
                     ADLFREPKRAAGFYRSQCDPAEEVVVEPAIHWAPSDEAGGLAKLVVCSNCEHLKFYVS
                     GNLIRELDPDRTQFPHLPHPPFVLNFGDELDGAWGDLRIEGYIGGNQVIVKNYSGRGV
                     DRQFQLLPDDAALIADGADSTRVVFRVTDEFGNVRPFANEAIKLELDGPAEIIGDNPF
                     ALVGGIGAVWIRAREQAGAVRLKATHPILGERHTIIEISAAPMEGV"
BASE COUNT          765 a         1292 c         1366 g          746 t
ORIGIN      
        1 cgtggtggga tgtcccaccg gcgcaggtgt cgcctcggcc cgaagtcaac accgcccgca
       61 cggcctacga cctcaaggtc ggacaacaga acgaaggaca atgacgtcat ggccattcgc
      121 ttcggtgtca gtcccatcgc ctggatcaat gacgacatgc cggaactcgg cggcgacacg
      181 cccgtggaaa cggtgcttgc cgatgcacga gacctgggtt tcaccggcat agagcttggc
      241 ggtaaattct ccaaggatcc agccgtcctc gaaccgctgc tggcggccca tggtcttggc
      301 ctggtcggcg gttggtacag cggcaacctc ctctcgcatg gtgcgcggga cgaaatcgca
      361 tccctgcaat ctcatcttgc cctgctcaag gccatgcgct gcacggtctt cgtccatgcg
      421 gagaccagca atgccgttca tggtgaccgc gcgcgaccgc tcgcggccac gccgcaactc
      481 gatgcgggtg cgtgggcgca gtttggagaa cgcctgaccg aggtcgccga ctacatcgcg
      541 agccagggtt tacggtatgc ctaccatcac catctgggaa ccgtggtcga tgggctcgac
      601 gatctggaaa acttcatgcg atataccggc gccacggtgg gtctgacggt ggacacgggt
      661 catgctgcac tggccggcgt cgatccggtg gaactgatcc ggtcctttcc cgaacgggtg
      721 gcgcatgttc actgcaagga catccggggc gaggtgttcc gcgacgtcat gctccggcac
      781 ggcagttttc tggaaggggt attggcaggg atgttcaccg tgcccggcga cggtgatttg
      841 gactatgacg cagtgctgcg ggcgctggcc ggtatcggtt acggcggatg gatcgtcgtc
      901 gaggccgagc aggatccggc caaggcgaat ccgcgccgat acgcccgagt gggattgcgc
      961 acgctgcggc aggccgctgc tgcggccggt ctggccgaag ccggattgca tgtatgcgag
     1021 ccgtgcgacg cacgcgagcc gcgcgatgca tgcgggccgc gcgatgcatg cgagccgcgc
     1081 gatgagtaag ttgctcgtcc ggtctcgcga gcccgacgaa aacggctgca tcctggaggt
     1141 cactccggcc agcgccggct ggagccgtgt gggattcaag gtctgccggc tgaccgtggg
     1201 ccaacgcatc gaaggtttca gcgccgatcg tgagacttgc atcgtgctgc tcaagggaaa
     1261 tgcggcgatc cgtacggcgg accgcgactt cggatcgatc gccagccgtg ccagcgtgtt
     1321 cgacgatgcc gcccccggtg cggtctatgt tccagccggc attcgctacg gcgtagcggc
     1381 caggacgctt gccgaggtcg ccatctgctc agcgcccggc ggtcgtggcg gcgaatcgcg
     1441 cctgatcgcc gcgtcgaaga tgctttgcga ggttcgcggt caggggacca acacccgcta
     1501 tgtgcgcaac attcttccgc aaaccgcgcc ggccgatagc ctgctcgtgg tggaggtgat
     1561 tacacccggc ggccattggt ccagctaccc gccgcacaaa cacgacagcg gagccgacgg
     1621 cgaggagacc gccctcgagg aaacctacta tcaccggctg gaccccccgc aaggctttgc
     1681 cgtgcagcgc gtctacaccg aagatggcag cctggacgaa acgctgtgcg tgcaggacgg
     1741 cgacgtcgtg ctggtgccgc gcggttacca tccggtcggg gcggcgcacg gctacacgct
     1801 ctattacttg aacgtgatgg ccgggccgga gcgcaagtgg attgcgcgca atgaacctcg
     1861 ccatgactgg atcgcacgca agaaatgacc cgtacaccgc ccttctgcgc gccaacgtcg
     1921 ccaaccgtgg gtaccgtgcc tatcgcactc cttgcgctcg ctgcgttcag ggcgcgcgcc
     1981 gcccctacgc ttctcacagc gcgcacggtg ctcatcgcgt tcattgcgct cacggcgcgc
     2041 atcatgctca cggtactcgc gctcgcaagc gttgcagccg cggcaggcaa cttcgccgag
     2101 gtcacgccga gcccgcagca agtgagttgg caggatctcg aatttggcgt gctggtgcat
     2161 tttggaacca acacctatct ggatcgcgag tggggcgacg gcacggcgcc gccggcggta
     2221 ttcaatccgg acaaattcga tccggagcaa tgggtgctgg cagcccaggc cgcaggggcg
     2281 cagtacctgg tgatggtcgc caaacaccac gatggatttt gtttgtggcc gaccgaagag
     2341 agcgactatt ccatcaaggc aagtccctgg aagaacggcc acggcgatat ggtggcggag
     2401 gtcgccgcag cggcgcgtaa gcacggcatg aaattcggcg tgtatctttc gccgtgggat
     2461 cgtcacgagc ccaaatatgc cgacgcagcc gcctatgacg agcactacca ggcacagctg
     2521 cgtgaactcg cgggaaatta cggtgagctg gccgaattct ggctggacgg tgccggcagc
     2581 cagggccatg tatataactt tgaccggtac gtcgaaacgc tgcgcatcta tcagccgaac
     2641 acgctgatct tcgcggatgt cgcacttctc gactacggcg acgctcgatg ggccggcaat
     2701 gaggacggcg tggtcaacgg tgaagactgg aatgtcgtcg accgtcacgg attgctgcgc
     2761 tggcggccgg tcgaagccga tacgcctctg cgcaagaact ggttctggca tgcgagcgac
     2821 gctgccaagg gggtcgagaa actcgtgcag atctacgaaa ccagcatcgg gcgcggcggg
     2881 cagctgatgc tcggcctttc gcccgacatg catggattgc tgtcggccgg cgatgtggcg
     2941 cggttgcgcg aattcggcgc ggcgatccgt gcgctctatg gcgacaatct catgcgcaag
     3001 cgccatgctg tcgacgaccc ggagctcgaa cgcgcgctgg acgacgaccg tgagacattt
     3061 tggtcggcgc cggccggcag ccatcatgca acgatcgaag tgcgcttcga taagccggtg
     3121 acgttcgacc gtgccatgac catggaatgg ctcgagggtg gacagcgcgt gcacgcgtac
     3181 cgcgtcgagg tctggaccgg cgcgggctgg aagacggttg cacaggcgca ggccatcggc
     3241 cacaagaaga tcgacatctt cgatccgatc acggccgacc gggttcggtt gaatctgctt
     3301 tcgacgaccg atgcagcgca gatccgcgag tttgctctct acgactcgcg ggccctacat
     3361 atctcccggg gaactaaacg ccttccatag gcgctgcaga gatttcgatg atggtgtgcc
     3421 gttccccaag tatggggtgg gtggccttga gccgcaccgc gccggcttgt tcccgcgcgc
     3481 gaatccacac ggccccgatg ccgcccacga gcgcgaaggg attgtcgccg atgatttccg
     3541 caggtccgtc gagttccagc ttgattgcct cgttggcgaa cggccgcacg ttgccgaatt
     3601 cgtcggtgac gcggaacacc acgcgggtgg agtcggcgcc gtcggcgatc aacgcggcat
     3661 cgtccggcag aagctgaaat tgccgatcga cgcctcgtcc ggaataattc ttgacgatca
     3721 cctggttgcc tccaatgtag ccttcgatac gcaggtctcc ccaagcgccg tccaactcgt
     3781 ccccaaaatt caagacgaag ggcggatgag gcaggtgggg aaactgcgtc cggtccgggt
     3841 cgagttcccg gatcaggttg cctgaaacat agaacttgag atgctcgcag ttcgagcaca
     3901 ccaccagctt cgcaaggccg ccggcctcgt cgctgggcgc ccagtgaatc gcgggctcca
     3961 cgaccacctc ttcggccgga tcgcattgcg agcgatagaa tcccgccgcg cgcttgggct
     4021 cgcgaaacag gtccgcgacg ccgtgatagc agatgcggtc tccggatccg aaatacccat
     4081 gcgtgttgta gtcgaaggcg caccaggcga tgccgccggc atattgcgga ttggaggcga
     4141 gctgatcgtg aatccgggca tggcgcagc
//
LOCUS       DATGEM010000144         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_987448, whole genome shotgun sequence.
ACCESSION   DATGEM010000144 DATGEM010000000
VERSION     DATGEM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987448"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VKQ27_04515"
     CDS             <1..424
                     /locus_tag="VKQ27_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88226.1"
                     /translation="VGQLQPGGRLLLWAMRRWIHAASQKTCPPGALAPAFARHGVLPA
                     LPHVHALLTELNCQARRRINLAPLAHHLIGDDEAVLLQLCRDANLTPPRAKATLALLL
                     DEEAIGAAFNALLIMVARLRDAGLGDVTIVPDGIAGAV"
     gene            463..1380
                     /locus_tag="VKQ27_04520"
     CDS             463..1380
                     /locus_tag="VKQ27_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLY88227.1"
                     /translation="MSDLDRLLNRALKWRDQPLALATVISTWGSAPRPRGSHMLVAAD
                     GRFEGSVSGGCVEGEILANAADVIAGTGFRRLRYGVADAAAWEVGLPCGGEIEVIVQP
                     VTEAGFDPIWFDRIAASREAGETLTLATDLDTGKTSTHPTEHAFLNAYAPPRRLLIVG
                     AVQIAQSLAALGRELGMAVTVIDPRGRFLTDDRFPGVTLDDRWPDEAVAAARPDPATA
                     IVTLSHDIKVDDPALVAALRSNAGYIAALGSRRSHAKRVERLTEIGFDTAAIARIEGP
                     AGLDIGAIGPAEIALSIAAGVVRTLSGRH"
     gene            1424..2953
                     /gene="cysS"
                     /locus_tag="VKQ27_04525"
     CDS             1424..2953
                     /gene="cysS"
                     /locus_tag="VKQ27_04525"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLY88228.1"
                     /translation="MTLAGAPLTLFNSLTRAIEPFQPIDPKMVRLYSCGPTVYSTAHL
                     GNLRAYVFTDTLRRVLNWKGYATTHIINITDVGHLTSDADAGDDKMELAAQKSGTTIW
                     DVAARYTQLFKADLKALGILDPAIWSVATDHIQDMIAFARALEEQGATYEIDGGLYFD
                     VSKVPDYGRLAGHAQGGPEIGRIDTVEGKRHAADFAVWRRSAPGEHRQMEWLSPWGPG
                     APGWHLECSVMSIKYLGEQFDIHTGGIDHREIHHCNEIAQNQAYNNSSKTGANIWMHN
                     NFLVDRTGKMSKSKGGVATLGALTARGVHPLAYRLLCLGAHYRSELEFSLDAMAAALT
                     RLKRLAIAIGTLKAEGAIDTDSLRPSQEVGFGKGAPFDWQRRALEDGLSEQAREFIAR
                     FDGALAADLMTPQALPVLDAVIASSLPTTEKLRLIASFDLVLGLGLLDLDRRALSVRP
                     EDASLDDADVDALLDERQKARAARDFAAADAARDRLKVAGVEIMDGDPLRWEWRPLLT
                     D"
     gene            3042..3467
                     /locus_tag="VKQ27_04530"
     CDS             3042..3467
                     /locus_tag="VKQ27_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HLY88229.1"
                     /translation="MAVKDLTRRRLLSIVGTRPEAIKIAPLALAATGRGMIEHELLAT
                     GQHDLLFDEAPAGFGLTADHRLAPVARDPLPDVMLERLTHALLSVLPAIDPDLLIVQG
                     DTNSALAGALAASAARTFDRPCRGRPALSRHRVPLARRA"
     gene            3439..4227
                     /locus_tag="VKQ27_04535"
     CDS             3439..4227
                     /locus_tag="VKQ27_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HLY88230.1"
                     /translation="MAYPWPEERNRVAIDHLADWLFAPTMAATTNLYNEPDVQGRIVV
                     TGNTGIDALLAMRARLPAPSSVRDTASLVFLTWHRRENIGAGIENICTAALQLADRGD
                     IRLLCSVPPKPRSGIHGQGAPWPTPRDYADGSAGLHRNRRNHGLGDPDPHRQRRHPGR
                     SPALGVPTLVLRDVTERPEALASGNLALVGTNPDRIVAAAARLFDDPQTHAAMARPSF
                     PFGQGDAAEKILDALEQYFSSGPCRDHPLPFAPSWIMDGGPKGA"
     gene            4230..>4590
                     /locus_tag="VKQ27_04540"
     CDS             4230..>4590
                     /locus_tag="VKQ27_04540"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HLY88231.1"
                     /translation="MAAPWAPESWRGYEARQLPEYPDPTALAAAEQQISRFPPLVFAG
                     EARALTAELAEVAAGKGFLLQGGDCAESFAEFHPNNIRDTFRVILQMAVVLTYASKLP
                     VVKVGRMAGQFAKPRSTA"
BASE COUNT          758 a         1581 c         1435 g          816 t
ORIGIN      
        1 ggtcggccag ctacagcccg gcggtcgcct tctcctctgg gcgatgcggc ggtggattca
       61 tgcggccagt cagaagacct gccctccggg tgcgctggcg cccgccttcg cgcgtcatgg
      121 cgtccttcct gctttgcccc atgttcatgc gttgctcacc gagctcaatt gccaggcgcg
      181 ccgtcgcatc aacctggccc ccctcgccca tcatctgatc ggtgacgacg aggccgtcct
      241 gctccagctt tgccgcgatg ccaatctcac cccgccgcgt gcgaaagcga cgcttgcgct
      301 tttgctggac gaggaggcta tcggcgctgc attcaacgcg ctcctgatca tggttgcccg
      361 gcttcgcgac gctggtctgg gcgacgtcac tatcgtaccc gacgggatcg ccggggcagt
      421 ttgatccctt gcgtggtgcc atgccccggc tagaccatgg gcatgtccga cctcgatcgc
      481 ctcctcaacc gtgccctcaa atggcgagac cagccgctcg cgctcgccac cgtgatttcg
      541 acctggggct ccgcgccgcg tccgcgtggc agccacatgc tcgtggccgc cgatggacgc
      601 ttcgagggat ccgtttcggg cggatgtgtc gagggcgaga ttctggcgaa tgctgcggac
      661 gtgattgccg gcaccggttt ccggcgcttg cgctacggcg tagcggacgc cgccgcctgg
      721 gaagtgggcc tgccctgcgg tggcgagatc gaggtgatcg ttcagcccgt caccgaagcc
      781 ggcttcgacc cgatctggtt cgaccgcatc gccgcgtcgc gcgaggctgg cgagacgctc
      841 accctcgcga ccgatctcga caccgggaag acgagcacgc accccaccga gcacgccttt
      901 ctcaacgcct atgcgccgcc ccgccgcctg ctgatcgttg gcgcagtgca gatcgcgcaa
      961 tcgctggcgg cgctcggccg ggaactcggc atggcggtga cggtgatcga cccgcgtggc
     1021 cgcttcctga ccgacgatcg ctttcccggc gtcacgctgg acgaccgctg gcctgacgag
     1081 gccgtcgccg cggcccggcc cgacccggcc accgcgatcg tcaccctgtc gcacgacatc
     1141 aaggtcgacg acccggcatt ggtggcggcg ctccgctcca atgcgggcta tattgccgcg
     1201 ctcggatctc gccgcagtca tgcgaaacgc gtggagcgcc tcaccgagat aggcttcgac
     1261 accgccgcga ttgcacgcat cgagggcccg gccggcctcg acatcggcgc catcgggcct
     1321 gccgaaattg ccctgtcgat cgccgctggt gtcgtccgga cgctttctgg cagacactga
     1381 gccctattcc atatcggcaa gaaaagcgtg tatcggcgcg cccatgacac ttgctggcgc
     1441 cccgctcacc ctcttcaaca gcctcacccg cgcgatcgag ccgttccagc cgatcgatcc
     1501 gaagatggtg cggctttatt cctgcgggcc gacggtctat tccaccgccc atctcggcaa
     1561 tctgcgcgcc tatgtgttca cagacacgtt gcggcgggtg ctgaactgga agggctacgc
     1621 gaccacccac atcatcaaca ttaccgacgt cggccacctg acctccgacg ccgacgccgg
     1681 tgacgacaag atggagctgg cggcccagaa gtccgggacg acgatctggg acgtggcggc
     1741 gcgttacacc cagctgttca aggccgatct caaggcgctg gggatcctcg atccggcaat
     1801 ctggagtgtc gcgacggacc atatccagga catgatcgcc ttcgcccgcg cgctcgagga
     1861 acagggcgcg acctacgaga tcgacggcgg cctctatttc gatgtctcca aggttcccga
     1921 ttacggccgc ctcgccggcc atgcacaggg tggccctgaa atcgggcgga tcgataccgt
     1981 cgagggcaag cgccacgccg ccgacttcgc cgtctggcgc cgatcggcac cgggcgagca
     2041 tcggcagatg gagtggctgt cgccatgggg tccgggcgcg ccgggctggc atctagaatg
     2101 ctcggtgatg agcatcaaat atctcggcga gcagttcgac atccacaccg gcggcatcga
     2161 ccatcgcgag atccatcact gcaacgagat cgcgcagaac caagcgtaca acaactcgtc
     2221 aaagacgggc gccaacatct ggatgcacaa caactttctg gtcgatcgga ccggcaagat
     2281 gtccaagtcg aagggcggcg tggcgacgct gggtgcgctg acggcgcggg gcgtgcatcc
     2341 cctcgcctac cgcctgctct gccttggcgc ccattatcgt tcggagctgg agttttcgct
     2401 cgacgccatg gcggccgctt tgacccgcct caagcggctg gcgatcgcga tcggcacgct
     2461 caaggcggaa ggcgcaatcg atacggacag tctgcgccct tcgcaggagg tcggcttcgg
     2521 caagggtgct ccattcgact ggcagcgccg cgcgctcgag gatggcttgt ccgagcaagc
     2581 gcgcgagttc atcgcccgct tcgatggcgc cttggcggcc gacctgatga cgccccaagc
     2641 tctgcccgtg ctggatgccg tcatcgcatc gtcgctcccg acgaccgaaa agctgcggct
     2701 gatcgcgagc ttcgatctcg tgctggggct cggcctgctc gatctcgatc ggcgcgcgct
     2761 gtcggttcgg cccgaggatg cgagcctcga tgatgcagac gtggacgcgc tgctggacga
     2821 gcgccagaag gcccgcgccg cccgcgattt cgccgctgcc gatgccgcac gggatcgcct
     2881 caaggtcgcc ggcgtcgaga tcatggacgg tgatccgctc cgctgggagt ggcgaccact
     2941 cctgacagac tgatcagatc cagatcgttt tgtctccgaa atgactagcc atcgccccca
     3001 caccccgctt gtcaaaggac gggccggcag ggggggatac aatggctgtc aaggatctca
     3061 cgcgtcgacg actcttgagt atcgtcggta cccgacctga agcgatcaag atcgccccgt
     3121 tggctttggc ggcaacgggg cgcgggatga tcgagcacga gcttctcgcc accgggcagc
     3181 atgatctcct gttcgacgaa gcgcctgctg gtttcgggct cacggctgac catcggctgg
     3241 ctccggtcgc ccgggatcca ttgccggatg tcatgctcga gcgtctcacc catgcgcttc
     3301 tttccgtcct gccggcgatc gatccggatc tgttgatcgt ccagggtgac accaattcgg
     3361 cgctggccgg cgcgctggcc gcttcggcgg ctcggacttt cgatcgccca tgtcgaggca
     3421 ggcctgcgct ctcacgacat cgcgtacccc tggcccgaag agcgtaaccg cgtggccatc
     3481 gaccacttgg ccgattggct tttcgctccg acaatggcgg cgaccacgaa cctctacaac
     3541 gagccggacg tgcaggggcg catcgtcgtg accggcaaca ccggcatcga cgcgctactg
     3601 gcgatgcgag cacgcctgcc cgctccgtca tccgtccggg ataccgccag tctggtcttc
     3661 ctgacttggc atcgacgcga gaacatcggt gcagggatcg agaatatctg caccgccgcg
     3721 ctccagctgg cggatcgggg agacatccgc ctactctgct cggttccccc caaaccccgc
     3781 agtgggatcc acggtcaggg agcgccttgg ccgacacccc gcgattacgc tgacgggagc
     3841 gctgggctac accgaaaccg tcgcaatcat ggcctcggcg accctgatcc tcaccgacag
     3901 cggcggcatc caggaagaag ccccgcgctc ggcgtaccga cgctggtgct ccgcgacgtc
     3961 accgaacgcc ccgaagcgct cgccagcggc aatctggcgc ttgtcggcac caatcccgat
     4021 cgcatcgtcg cagccgccgc acgactgttc gatgatccgc agacacacgc tgccatggcc
     4081 cgcccgagct tcccattcgg gcaaggggat gctgcggaga agatcctcga tgccctcgaa
     4141 caatatttct cgtcaggccc ttgccgcgat catccattgc ctttcgcgcc atcgtggatc
     4201 atggacggcg gtcccaaagg agcatgatga tggccgcacc atgggccccc gaaagctggc
     4261 gcggatatga agcgcgacag ctgcccgaat atcccgatcc gaccgcgctg gccgcggccg
     4321 agcaacagat ttcgcgtttc ccgccgctcg tctttgccgg tgaagctcgc gctctgacgg
     4381 cggagctggc ggaggtcgca gccggcaagg gatttctact gcagggcggc gattgcgcgg
     4441 aaagcttcgc agaatttcat ccgaacaaca ttcgcgacac cttccgcgtc attcttcaga
     4501 tggcggtggt gctgacctat gcctcgaagc tgccggtggt gaaggtcgga cgtatggcgg
     4561 gccagttcgc caagccccgc tccacggcca
//
LOCUS       DATGEM010000145         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_323430, whole genome shotgun sequence.
ACCESSION   DATGEM010000145 DATGEM010000000
VERSION     DATGEM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323430"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="phnK"
                     /locus_tag="VKQ27_04545"
     CDS             <1..668
                     /gene="phnK"
                     /locus_tag="VKQ27_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HLY88232.1"
                     /translation="VGESGSGKTTLLNVLSGRQAPDAGAVWYRDPAGHLHDVHAMPAP
                     ALRTLHRSDWGFVHQDPRQNLRMNVTAGGNVGERLMAQGARHYGNIRDQAIDWLKQVE
                     IDFERVDDMPRTFSGGMLQRLQIARTLVTHPRLVFMDEPTGALDVSVQARLLDLIRSL
                     VTRLQIAVVLVTHDIGVARLLAHRIMVMQRGRVVETGLTDQVLDDPQHPYTQLLVSSV
                     LQA"
     gene            1012..1713
                     /gene="phnL"
                     /locus_tag="VKQ27_04550"
     CDS             1012..1713
                     /gene="phnL"
                     /locus_tag="VKQ27_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HLY88233.1"
                     /translation="MSPVVLRTDALAKTFTLHLQGGVRIPVLSGVGLEVHAGECVVLS
                     GPSGVGKSTLMRSLYGNYRTETGHILVRHRGKMVDLANADPRTILAVRHETLGYVSQF
                     LRVVPRVPALDVVAEVLVGRGTAQEEARRVATDLLLRLNIPRRLHSLPPATFSGGEQQ
                     RVNLARGFIGNHPVLLVDEPTASLDAQNRTVVVAMINEAKARDIAIVAICHDAEVRDA
                     IADRLFELTPLQDAA"
     gene            1710..2843
                     /locus_tag="VKQ27_04555"
     CDS             1710..2843
                     /locus_tag="VKQ27_04555"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HLY88234.1"
                     /translation="MTDDTILTNAVLVLPDAMLHGTMVICGDTIADIQPHRSSALAAH
                     DMDGDYLIAGIVDTHTDNLERQVLPRSLARWPSRSAMVAHDAQCAAAGVTTVFDALCL
                     GDLGFDKDRIRTFQEGVIDLDVLTEADLLKAEHFLHLRCEVPALDMLELFDSVADHTL
                     VRMVSLMDHSPGVGQYANLDFYRALRRRGGLDDEYIERRILELQEQRQRTRIPHRRAL
                     LDRIAARNIAIASHDDRTEEEIAENAADGIDISEFPVTMEAARAAKAAGMQVIAGAPN
                     IVRGGSHSGNVAAADLLENGAVDAFASDYVPNSLVEAAFHCAQRIGLPEAIALVTENP
                     ARLAGLDDRGRLEPGQRADVARVRLHEGLPVVRQVWRAGERVA"
     gene            2840..3514
                     /locus_tag="VKQ27_04560"
     CDS             2840..3514
                     /locus_tag="VKQ27_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HLY88235.1"
                     /translation="MMRVAVYYAPQSDDPLSAEAATWLGRDPDSGAPLPQPDIANIAE
                     ATAEPRLYGFHATLKPPMRLKDGRPWRDVVEAATELGNRIAPFELPRLAVSDVHGFLA
                     LRETEPCAPLQALADTCVEQLDHLRAPLTEDEIARRRRAPLTAQQDAMLLRWGYPYVF
                     ETWFFHMTLTRRLNADEKHLFMPAAEAYFARVIALPRQVSDICLFAQPGPGEPFVIRE
                     RVKLRG"
BASE COUNT          648 a         1143 c         1154 g          652 t
ORIGIN      
        1 tggttggtga atccggttca ggcaagacga cgctcttgaa cgtgctgtcc ggccgccagg
       61 cacctgatgc aggtgccgta tggtatcgcg atcctgctgg ccacctgcac gatgtgcatg
      121 caatgccggc accggcgttg cgcacgctgc atcgatctga ctgggggttc gtgcaccagg
      181 atcccaggca gaacctgcgc atgaacgtca cggccggcgg caacgtcgga gagcgcctga
      241 tggcgcaggg cgcgcggcac tacggcaaca tccgcgacca ggcgatcgac tggctgaagc
      301 aggttgagat tgacttcgaa cgcgtggacg atatgccgcg cacattctcc ggcggcatgc
      361 tgcaacgttt gcagatcgcg cgcacgctgg tgacacatcc gcgtctggta ttcatggacg
      421 agccaacggg cgcactcgat gtgtcggtgc aggcgcggtt gctggacctg atccgttcac
      481 tggtaacacg gctgcagatc gccgtcgtgc tggtcacgca cgatatcggc gtggcacggc
      541 tgctggcgca tcgcatcatg gtgatgcagc gcgggcgtgt ggtggaaacc ggactgaccg
      601 accaggtgct ggatgacccg cagcatcctt atacgcagct gctggtcagc tcggtgttgc
      661 aggcatgaac aggctgacgg aaatgctgtt gccccggcac gatgggctgt ggtacctagg
      721 cgtccggaag gcgacaaaag ccgccctccg gagcccggtg gttccgaggc ggatcaagat
      781 tgcgacccgg atccgctccc tcccccaccg gagcgatacc cagacgcgaa agcgaatggt
      841 tctcctccga tggcaagctt gtggtgaccg gacaggatgc ccggtcatcc gggcaagccg
      901 cgaccgacat gcgtgcgtac aagcgcgtgc ggtttcgtcc ttcaggactc aacaggcggc
      961 attgagccat ccccgaagtt tgcgctgcag acctgaagaa ggcgttgcca catgagcccg
     1021 gtcgtgctgc gcaccgatgc gctcgccaag accttcaccc tgcatttgca gggaggcgtg
     1081 cgcatccccg tcctgtccgg cgtcggcctg gaagtccacg caggcgaatg tgtggtgctg
     1141 tcgggaccat ccggggtcgg caaatcaacc ctgatgcgca gcctctacgg caactatcgt
     1201 accgagacag ggcacattct ggtccggcat cgcggcaaga tggtcgatct ggcgaacgcc
     1261 gacccaagga ccattctcgc ggtacgccac gaaacgctcg gctatgtcag tcagttcctg
     1321 cgcgtggtac cccgcgtgcc tgccctcgac gtggtcgcgg aagtgctggt aggacgtggc
     1381 accgcccagg aagaagcccg acgcgtcgcg acagacctgt tgctgcgatt gaacatacct
     1441 cgccggctgc acagtctgcc accagccacg ttttccggcg gcgagcagca gcgcgtcaat
     1501 ctggcacgtg gtttcatcgg caaccacccc gtgcttctgg tcgacgaacc cacggcttcg
     1561 ctggatgccc agaatcgcac cgtggtagtc gccatgatca atgaggcaaa agcgcgcgac
     1621 attgccattg ttgccatctg ccacgatgcc gaggttcgcg acgctattgc cgatcggctg
     1681 ttcgaattga cgcctttgca ggatgccgca tgactgacga cacaatcctg accaacgcag
     1741 tcctggtgct gccggacgcg atgctgcatg gaaccatggt catatgcggt gatacgatcg
     1801 cggacattca gccacatcgt tcgtcggcgt tggcagcaca cgacatggac ggcgattatc
     1861 tgatcgccgg catcgtcgat acccacaccg acaatctcga acgccaggtg ctgccgcggt
     1921 cgctcgcgcg ctggccgtca cgctcggcca tggtcgctca cgacgcacaa tgcgccgccg
     1981 ctggtgtcac cacggtcttc gatgcgctgt gccttggcga tctcggcttc gacaaggatc
     2041 gcatccgcac attccaggaa ggcgtgattg acctggatgt gctgactgaa gccgacctcc
     2101 tgaaggccga acacttcctc catctccgct gcgaggtgcc agccctcgat atgctggagt
     2161 tgttcgacag tgtcgccgat cacacgctgg tccgcatggt cagcttgatg gatcacagcc
     2221 cgggcgtcgg ccaatacgcc aatctcgatt tctaccgcgc cttgcggcga cgcggcggcc
     2281 tcgacgatga gtacatcgag cgccgtatcc tcgaactgca ggaacagcgc cagcgcacgc
     2341 gcattccgca tcgccgcgcg ctgctcgatc gcatcgcggc ccgcaatatc gcgattgcca
     2401 gccacgacga ccggaccgag gaagagatcg cggaaaacgc cgcggacggt atcgatatca
     2461 gcgaattccc cgtcaccatg gaggcggcgc gcgcggcgaa ggccgccggc atgcaggtga
     2521 tcgcgggtgc gccgaacatc gtgcgcggcg gctcgcattc cggcaatgtt gccgcggccg
     2581 atctgctgga gaacggcgcg gtcgacgcct tcgcctccga ctacgtgccg aacagcctgg
     2641 tggaggccgc gttccactgc gcacagcgca ttggcctgcc cgaggcgatc gcgctggtca
     2701 cggagaatcc ggcacgactg gctgggctgg acgatcgtgg ccggctggag cctggccaac
     2761 gtgccgatgt tgcgcgcgtc cggctgcatg aaggactgcc ggtggtgcgc caggtgtggc
     2821 gggcgggtga acgcgtcgca tgatgcgtgt tgccgtctat tatgcgccac agtccgacga
     2881 cccgttatct gccgaggcag ccacgtggct cggccgcgat cccgacagtg gagcgccatt
     2941 gcctcagccg gacattgcga acattgccga ggcgacggca gaaccgcggc tctacgggtt
     3001 ccatgccacg ctgaaaccgc cgatgcggct gaaggatggg cgaccgtggc gcgatgtggt
     3061 ggaagcagcg actgaactcg gcaaccgaat tgcgccgttc gaattgccgc ggcttgcggt
     3121 atccgatgtg cacggttttc tggcgttgcg cgagacggag ccttgcgcgc cgttacaggc
     3181 tctggctgac acctgcgttg aacagctcga ccacctgcgc gcgccactga ctgaagatga
     3241 gatcgcacgc cgccgccgcg cccccctgac cgcgcagcag gacgcgatgc tgttgcgctg
     3301 gggctatccc tacgtgttcg agacgtggtt cttccacatg acgctgacac gccggctgaa
     3361 cgccgacgag aagcacctgt tcatgccggc ggcagaagct tatttcgctc gggtgatcgc
     3421 cttgccccgc caggtttcgg acatctgcct gttcgcgcaa cccgggcctg gcgagccgtt
     3481 cgttatccgg gagcgtgtga agctgcgcgg ttgagcgcag caagaaaacg cgccactccc
     3541 tcggcaacag agctgtcgtt ggtcacggtt tcgacatgta catcagcggg cagtgga
//
LOCUS       DATGEM010000146         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1671692, whole genome shotgun sequence.
ACCESSION   DATGEM010000146 DATGEM010000000
VERSION     DATGEM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671692"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VKQ27_04565"
     CDS             complement(<1..300)
                     /locus_tag="VKQ27_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88236.1"
                     /translation="MFDLAIIGGGINGCGIARDASGRGLSVLLVEQGDLASATSSAST
                     KLIHGGLRYLEHYAFRLVHEALAEREVLLRAAPHLIRPMRFILPHHAGLRPWWLIR"
     gene            complement(382..852)
                     /gene="moaC"
                     /locus_tag="VKQ27_04570"
     CDS             complement(382..852)
                     /gene="moaC"
                     /locus_tag="VKQ27_04570"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLY88237.1"
                     /translation="MTDFTHFDARGHATMVNVGAKPITERTATARARVTMLPATADLI
                     RTGGAKKGDVLGVARIAGIMAAKRTSDLIPLCHPLPISAVTLDLEATEAAVEIVATVR
                     TTGQTGVEMEALTAASVAALTVYDMCKSVDRAMHIEGVRLTHKAGGKSGEFVQD"
     gene            complement(849..1670)
                     /gene="trpC"
                     /locus_tag="VKQ27_04575"
     CDS             complement(849..1670)
                     /gene="trpC"
                     /locus_tag="VKQ27_04575"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HLY88238.1"
                     /translation="MPDTANIAPGDVLTRICTDTRAQVALAKSKVSLESLRAKIGNRG
                     DEPRGFGSALKQSVAEGRYGLIAEIKKASPSGGLIRLDFDPPALAQAYKDGGATCLSV
                     LTDAPYFQGSPEHLKAARAAVDLPVLRKDFILDEWQVYESRAMGADCILLIMAALTDT
                     EARDLETLARDLDMDVLAEVHNQRELDRALGLQTPLIGINNRNLKTLVTDLSVTEELA
                     PHVPADRFLIAESGIRSHDDVKRLLAAGSQCFLVGETLMRQPDVTAATRALLGDE"
     gene            complement(1675..2694)
                     /gene="trpD"
                     /locus_tag="VKQ27_04580"
     CDS             complement(1675..2694)
                     /gene="trpD"
                     /locus_tag="VKQ27_04580"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HLY88239.1"
                     /translation="MSNNLKPVLARLAAGAKLTDDEAENAFDIIMSGEATPAQIGALL
                     MAMRVRGETVPEITGAVRAMRARMTAIAAPPDAIDVCGTGGDAAGTLNVSTAVTFVIA
                     GCDVPVAKHGNRALSSRTGGADVLTALGINVDVPLERLSDILRAANCAFLFAPRHHPS
                     MRHAAGPRVELGTRTIFNLLGPLANPARVRRQFTGVFAPEWAEPMAETLGRLGTETAW
                     IVHGQGLDELTVAGENSVVAWQNGGIRRFTITPEDAGLPRAPIEAIRGGDAALNAATL
                     QALLQGAAGPYRDTVVLNAAAALIVAARAGDLREGVTLASRSIASGAALAALDTLRRE
                     TTSKD"
     gene            complement(3015..>3186)
                     /locus_tag="VKQ27_04585"
     CDS             complement(3015..>3186)
                     /locus_tag="VKQ27_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLY88240.1"
                     /translation="PTAWTEDGLVMGLRHRTLPIFGVQFHPESIASQHGHDLLANFLA
                     IARGTNEPAKAA"
BASE COUNT          504 a          920 c         1203 g          559 t
ORIGIN      
        1 gcggatcagc caccacgggc gcaggccggc gtggtgtggc aggatgaagc gcatcggacg
       61 gatcaggtgc ggggcggcgc ggagcaggac ttcgcgttcc gcgagcgctt cgtggaccag
      121 gcggaacgcg tagtgctcga ggtagcgcag gcctccgtgg atcagcttgg ttgaggcgga
      181 ggaagtggcg ctagcgagat caccttgttc gaccagcagg acagagaggc cgcggccgga
      241 ggcgtcgcgt gcgatgccgc agccattgat gccgccgccg atgatggcaa gatcgaacaa
      301 gaagaattat gaaaccgcag atggacacag atacacacag atcatctgtg cccatctctg
      361 tttatctgtg gttccattgc cttagtcttg tacgaactca ccggacttgc cgccggcttt
      421 atgggtcagg cggacccctt cgatatgcat tgcgcggtcg actgacttgc acatgtcgta
      481 gactgtgagc gcggcgaccg aggcagcggt gagggcttcc atctccacac cggtctggcc
      541 ggtggtgcgc accgtggcca cgatctccac cgctgcttct gttgcttcca ggtccagcgt
      601 cacagcgctt atcggcagcg gatggcagag ggggatcagg tccgacgtgc gcttggccgc
      661 catgatgccg gcgatgcgcg cgacacccag gacatcgccc ttcttggcgc cgccggtacg
      721 gatcagatcg gcagtggcgg ggagcatggt gacgcgggcg cgggcggtgg cggtgcgctc
      781 ggtgatgggc ttggcgccga cgttgaccat cgtggcgtgg cctcgggcgt cgaaatgagt
      841 gaagtcggtc actcgtcgcc cagcagggcc cgggtcgcgg cggtgacgtc gggctgacgc
      901 atcagggtct cgccaaccag gaagcactgc gagcccgcgg caagcaggcg cttcacatcg
      961 tcgtggctgc gaatgccgct ttcagcaatc aggaagcggt cggctgggac atggggggca
     1021 agctcttcgg tcactgaaag atcggtgaca agggtcttca ggttgcggtt gttgatgccg
     1081 atgagaggag tttgcaggcc gagggcacga tcgagttcgc gttggttgtg cacctcggcg
     1141 aggacgtcca tgtcgagatc gcgggccagc gtttccagat cgcgtgcctc ggtgtcggtg
     1201 agtgcggcca tgatcagcag gatgcaatcg gcgcccatgg cgcggctctc gtacacctgc
     1261 cactcatcga ggatgaagtc cttgcgcagc accggcagat cgaccgccgc gcgggcggct
     1321 ttcaggtgtt cgggactgcc ctggaaatag ggtgcgtcgg tgagcaccga gaggcaggtg
     1381 gcaccaccat ctttgtaggc ctgcgccaga gcgggagggt cgaagtcgag gcggatcagg
     1441 ccgccggagg gcgaggcctt cttgatctcg gcgatcaggc cgtaacggcc ttcggccaca
     1501 gactgcttca gggcggagcc gaagccgcgc ggctcgtcgc cacggttgcc gattttggcg
     1561 cgcagggact ccaggctgac cttggacttg gcgagcgcaa cctgtgctcg ggtgtcggtg
     1621 cagatgcggg tgagtacgtc gcctggggcg atattggcgg tgtccggcat ggcttcagtc
     1681 tttcgatgtc gtctcacgcc gcagcgtatc caacgcggcg agcgcggcgc ccgaggcaat
     1741 cgagcggctg gcgagtgtga cgccctcgcg aaggtcgccc gcgcgtgcgg caacgatcaa
     1801 ggcggcggcg gcgttcagca ctactgtgtc acgatatggt cctgcggcgc cctgcaacaa
     1861 cgcctgcaac gtggctgcat tcagggcggc gtcgccgcct cggatcgcct cgataggcgc
     1921 gcgggggaga ccggcatcct cgggggtgat ggtgaagcgg cggatgccgc cgttctgcca
     1981 ggcgacaacg ctgttctcgc cggcgacggt gagttcgtcg aggccctggc cgtggacgat
     2041 ccaagcggtc tctgtaccaa ggcggccgag cgtttcggcc atcggttccg cccattcggg
     2101 ggcgaatacg ccggtaaact gacggcggac gcgggccggg ttcgccagcg ggccgagcag
     2161 gttgaagatg gtgcgggtgc cgagttccac gcggggaccg gcagcgtgcc gcatcgacgg
     2221 gtgatggcgc ggcgcgaaca ggaaggcgca gttggcggcg cgcaggatgt cggaaaggcg
     2281 ctccagaggg acatcgacgt tgatgccgag ggcggtgagg acgtcagcgc cgccggtacg
     2341 cgaggagagc gcgcggttgc cgtgcttggc gacggggacg tcgcacccgg cgatgacgaa
     2401 ggtgaccgcg gtggagacgt tgagcgtgcc cgctgcgtcg ccgccggtgc cgcagacgtc
     2461 gatcgcgtcg gggggcgcgg caatggcggt catgcgggcg cgcatggcgc ggacggcacc
     2521 ggtgatctcg gggaccgtct cgcctcggac gcgcatcgcc atcagcaggg cgccgatctg
     2581 ggctggggtg gcctcgcctg acatgatgat gtcgaaggcg ttctcggctt cgtcgtcagt
     2641 gagcttcgca ccggcggcga ggcgggcgag gacgggcttc aggttgttcg acatgtcagg
     2701 cagcgctggc gagggcgagc atgcggcgca tccttgcctt gcggcgggca gcgggtcaac
     2761 gcggggttgg cgggagccgg cggatgggat cggcatcacg gcgactgtgg tccatggccc
     2821 gctgcaggtt gcgcaggtgg tttacgcgat ggtcgagaga gcaggcggag tgctctgcgg
     2881 aatgacggtg atgcgttcct gcgttcctgc cagccaaatt ggtgagggct ttatcctcat
     2941 gacactcggc gatgagcccg aatgggattg cttcgtcgcc acgctcctcg caatgacact
     3001 gtggtcgttg gacgtcacgc cgcctttgct ggctcgtttg tcccccgcgc gatcgccagg
     3061 aagttggcga gcaggtcgtg gccgtgctgg ctggcgatgc tctcgggatg gaactgcaca
     3121 ccgaagatcg gcagcgtgcg gtggcggagg cccatgacga ggccgtcctc ggtccaggcg
     3181 gtgggc
//
LOCUS       DATGEM010000147         9994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1115604, whole genome shotgun sequence.
ACCESSION   DATGEM010000147 DATGEM010000000
VERSION     DATGEM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115604"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VKQ27_04590"
     CDS             complement(<1..1494)
                     /locus_tag="VKQ27_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY88241.1"
                     /translation="MNDTVVTDSVKRFKWVGTRPIRPDGVPKVTGKALYGGDYKLPGM
                     LYGKILRSPHAHARIKSIDTSAAEKLTGVKAVITSKDLPEQKFDYIGPERVAQNFWHI
                     TRNIMAREKTLYEGHAVAAVAAISASIAEEAASLIKVDYEVLPHVIDVDDAMADNAPL
                     LFEDMITRGIEPAPTKPSNISKRLEFATGDVETGFKHADVVIEKEFKTAAVHQAYIEP
                     HACIARCDADGQTEIWSSSQGHFQMRALTAAILKKPVGDIRVYPAEIGGGFGGKTVTY
                     LEPVATLLSMKAGNPVRLMMTREDVFRATGPTSGSSMWVKIGVTKDGKIVAADGIFKF
                     QSGAFPGSPVMNGCLCAFAPYDIPNQRAVGYDVVSNRPKAAAYRAPGSPISAFAVESV
                     LDMCAQNIGMDPLELRHKNACRIGTPTIFGPKHAHDGYVQTLEALMNHPAYKVKLGPN
                     QGRGVASGYWFNGGGESSATIQVNVDGTVLVATGSPDIGGSRASMALM"
     gene            1663..2658
                     /locus_tag="VKQ27_04595"
     CDS             1663..2658
                     /locus_tag="VKQ27_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88242.1"
                     /translation="MRIVALEEHYTVPRVVAGIAPDAIARRGFPRPDFPWAQTIKRNE
                     LADLGDARLADMDASGITMQVLSVAGPGADLVPGRAGIDLARAYNDALAEAIARHSTR
                     YRGFAHLPMLAPDAAGDELERTVKKLGFHGALINGATDGMFLDEPRFAPILTRAEALG
                     VPLYLHPGIPAPAVRNAYYDNLPGNLSFTLALSAWGWHYDTAVHVLRLALSGTLDRYP
                     KLKIIVGHMGEALPFMMDRIDETTSAQAKSLLKRSVRQMIVDQVWITTSGFFTLVPFM
                     AALMTFGVDRIMFSVDYPFASNTRARAFLDALPVSPADRARIAHGNADELLRLPV"
     gene            complement(2663..4258)
                     /locus_tag="VKQ27_04600"
     CDS             complement(2663..4258)
                     /locus_tag="VKQ27_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88243.1"
                     /translation="MRHWFIAMCIGVLASGVPAVADETPKHGGTLTFMIPADAPPSFD
                     GHRENTFATIHAVAPFYSVLIRANPDNPADTTHFVCDVCVEIPQPTGGGKSYTFLIRD
                     DIKFSDGAPLTALDVAASWNKIVNPPEGVISVRRGYYAMIDKIEAADAKTVVFQLKFS
                     TDAFLPALADPYAFIYRKAILDRDPHWYETHILGSGPFRFKDYQLGQSISGVRSPDYY
                     RPGLPYLDGFIGIFADKQAVRVAAIKSDRAAIEFRGFPPATRDELVAALGDKITVQER
                     DWNCGALITPNHVRKPFDDARVRRALTLAIDRWHGAEPMSRITVSRTVGGIVFPGSPL
                     AATHAELQEIAGYWPDIEKSRAEARRLLHEAGADGLTFELLNRNVDQPYKFNGIWVVD
                     EWSKIGIHATQRVLPTGPYEEALRHGDFAVAVDAECQNIVNPLLDGTKYLPHSVSDAN
                     YGNYDDPEEVSLYDRMLLETDFSKQRALMRQFEKRVLDTEAHEAFLLWRYRIVPYRSY
                     VKGFRISPSHYVNQDLATIWLDR"
     gene            complement(4271..5317)
                     /gene="tsaD"
                     /locus_tag="VKQ27_04605"
     CDS             complement(4271..5317)
                     /gene="tsaD"
                     /locus_tag="VKQ27_04605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLY88244.1"
                     /translation="MRPVLGIETSCDETASAALDADGHILAEAVLSQECEHARYGGVV
                     PEIAARAHLAFLPEQVVRVMSQAGLRFADLGGVAASAGPGLIGGLIVGSQFAKGIALA
                     HRLPYVAVNHLEAHALTARLPGLVPDGLAFPYLLLLVSGGHCQCVAVEGVGHDVRLGS
                     TMDDAAGEAFDKVAKLLGLGWPGGPALEKLAAGGDARRYAFPRPLLGRSGCDFSFSGL
                     KTAVALEVGRLGSGALPAAVAADIAASFQHAVAEVLTDRAAHAMTMMRERSPEVRALV
                     VAGGVAANGAVRAALATAAETHDFELIAPPVRLCTDNAVMVAWAGIERLRLGLTNEMN
                     FAPRPRWPLEQLAM"
     gene            5361..6293
                     /gene="hemC"
                     /locus_tag="VKQ27_04610"
     CDS             5361..6293
                     /gene="hemC"
                     /locus_tag="VKQ27_04610"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HLY88245.1"
                     /translation="MITAMSPLRVGTRGSPLALIQTRMVIDALRGRREAEPAVIRTTG
                     DSVQDRRLADIGGKGLFAKEIHDALAQGRIDAAMHSLKDLETDLPQGIVLACVLPRED
                     ARDALILGAAGPADPAQPFASLPHAARIGTASVRRQAQLLHVRPDLAMSTLRGNVDTR
                     LAKLAAGECAATLLAYAGLKRLGVADRASAILEPDIMVPAAGQGIVGVTVREQDEALR
                     ALLSDIADPDAMVAATAERALLAELDGSCRTPIGAHAVLLSDGALRLLAMVARVDGSF
                     LLRRSLQGPASDAVQLGRELAASMRADSPDDIFV"
     gene            6286..6996
                     /locus_tag="VKQ27_04615"
     CDS             6286..6996
                     /locus_tag="VKQ27_04615"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLY88246.1"
                     /translation="MSDPGASVLITRPEPGASETAARVAALGLLPVVAPLLQIHTLPG
                     AWPPSGQVQALVVTSGNAIGALPASHRHLLLLAVGQATARRARAAGFEQVESADGDAS
                     ALVDLAERVCQKQRGPLLLATGRRQGNSIAEELRRRGFAAVRRVVYSADPVALLPASA
                     RSALRSGALRAALFFSAETARAGVRLIESAALLDAVRSVDAVAIGKPAAVALEALPWR
                     RVRVAARPDQDAMLALLQ"
     gene            6993..7958
                     /locus_tag="VKQ27_04620"
     CDS             6993..7958
                     /locus_tag="VKQ27_04620"
                     /inference="COORDINATES: protein motif:HMM:NF021264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitofilin family membrane protein"
                     /protein_id="HLY88247.1"
                     /translation="MSESSTLPAPEPEPTPEPPGAALPARHSILPWLSAAGFVVLAGA
                     IVWVWQNPLLPPPSAQTGALEQQVVALEARVARLEKQPPITAPNLTTLEARVAALEQR
                     QPAAAAPPPNLGPLTARLEALEHQQAADLAPMEVRISRLEDAGHQVLTDVTQKLASED
                     ARFAALEKSSSRAVKISAARQALDAGHKLGDVSGAPPALARYATIDPPTEASLRLAFP
                     ATSQAALAASRPSVDGQPLLSRIWARAQDLVTIRRGDQVLVGDPAAGLLATARTALDA
                     GDLAGAVRSVGALTGPPAQAMAGWLAQARALLEARAALASWAAQG"
     gene            7958..9238
                     /locus_tag="VKQ27_04625"
     CDS             7958..9238
                     /locus_tag="VKQ27_04625"
                     /inference="COORDINATES: protein motif:HMM:NF018875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HLY88248.1"
                     /translation="MRRILFAVVAAAVVLAVAWALAAIPGRMTANIGDITFEAATSVV
                     ALGLLLGFVLLYSLFRLLGAAVRLPRSLRRRAAARRRKAGDVATTRTLLALAAGETGE
                     ARREAHRARRLLGDTPATLLLAAEAGRIAGRTDEAVAAFRTLSERDDAAFLGLRGLLR
                     DAVEREDWQEAAALARKAEAVQPGAAWLRRERARLAVRSANWSDALALADADAPKAAL
                     ATGAADAEADVIRATRLAKEAWQNDSSLAPAALAYARRLRAAGRESRALATVRHSWSI
                     APHPDLAAFALAPISDPLARMQAARQLTEANPEHAESRLLLARAALDAGLTGEARRYA
                     EAARAAGLNQRRLWLLLADIDEAESIDQRASLRHAATAEPDPTWRCGACHTAHAEWHP
                     GCPSCFTVGGLHWASRGPIAAPILQAPASETLLP"
     gene            complement(9260..9691)
                     /locus_tag="VKQ27_04630"
     CDS             complement(9260..9691)
                     /locus_tag="VKQ27_04630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLY88249.1"
                     /translation="MMMPPGQLHEISRGFGHGHTGIDLMAPYGSPIRAAAGGVVVYAG
                     WYFAYGKIVDIRHADGLITRYAHMSEFAPGIAPGAPVAEGDMIGQIGMTGRAHGAHVH
                     FEVRIDGHPVDPKPFLALAPCPRSAPEPLEEARAPDRRWDR"
BASE COUNT         1645 a         3274 c         3304 g         1771 t
ORIGIN      
        1 catcagcgcc attgaggcac gcgatccgcc gatgtccggg ctgccggtcg ccacgaggac
       61 ggtgccatca acgttcacct ggatggtcgc gctcgactca ccgccgccgt tgaaccaata
      121 gcccgaggcg acgccgcggc cctgattcgg ccccaacttc accttgtagg cgggatgatt
      181 catcaacgct tctagcgtct gcacgtagcc gtcatgcgca tgcttcggtc cgaagatcgt
      241 cggcgtgccg atgcggcagg cgttcttgtg ccggagttcc agcggatcca tgccgatatt
      301 ctgcgcgcac atgtccagca cgctctcgac cgcgaaggcc gagatcggcg agccaggtgc
      361 gcgataggcg gccgccttcg ggcggttgct cacgacgtcg tagccgacgg cgcgctggtt
      421 ggggatgtcg tacggcgcga acgcgcacag gcaaccgttc atcaccggcg agccggggaa
      481 cgcgcccgac tggaacttga agatgccgtc ggcggcgacg atcttgccgt ccttggtgac
      541 accgatcttc acccacatcg acgaaccaga tgtggggccc gtcgcacgga acacatcctc
      601 gcgcgtcatc atcaggcgca cgggattgcc cgccttcatc gaaagcaacg tggcgaccgg
      661 ttccaggtaa gtaacggtct tgccgccgaa gcctccgccg atctccgccg gatagacacg
      721 aatgtcgcca accggcttct taaggatcgc cgcggtcaac gcgcgcatct ggaaatggcc
      781 ttggctcgat gaccagatct cagtctgccc gtcggcatcg cagcgcgcga tgcaggcgtg
      841 gggctcgata taggcctgat gcaccgcggc ggtcttgaat tccttctcga tcaccacgtc
      901 ggcgtgtttg aagccggtct cgacgtcgcc cgtcgcgaat tcgaggcgct tcgagatgtt
      961 ggagggcttg gtgggcgcgg gctcgatgcc gcgcgtgatc atgtcctcga acagcagtgg
     1021 cgcgttgtcg gccatcgcgt cgtcgacgtc gatgacgtgg ggcaggacct cgtagtccac
     1081 cttgatcagc gatgcagctt cctccgcaat cgacgcgctg atggcggcga cggcggccac
     1141 ggcatggccc tcatacagtg tcttctcgcg cgccatgatg ttgcgcgtga tgtgccagaa
     1201 gttctgcgcg acgcgttcgg ggccgatgta gtcgaatttc tgctcaggca gatccttcga
     1261 cgtgatcacg gccttcacgc cggtcagctt ttcggcggcc gatgtgtcga tcgacttgat
     1321 ccgggcatgg gcatgtggcg accgcagaat cttgccgtac aacattcccg gcagcttgta
     1381 gtcgccaccg tagagggctt tgcctgtcac cttgggtacg ccgtccggac ggattggacg
     1441 ggttcctacc cacttgaagc gcttcaccga gtcggtcacg actgtgtcgt tcactgcacc
     1501 ctcgccctag tttgtgtgga ctgcctccga aggcaggaaa gtcgcgcagg gcgggtcgga
     1561 tggcaagtcc cgaccctcca ggttttctta ccgtgatctt cccgtttggc gtcgccgcgc
     1621 cgggcatgcg acgcttccgc cggtcgtcgc gggaggaatc acatgcgcat tgtcgctctg
     1681 gaagagcact acaccgtccc ccgggtcgtt gccgggatcg cgcctgacgc gattgcacgg
     1741 cgcggctttc cgcgcccgga tttcccctgg gcgcagacaa tcaagcgcaa cgaactggcc
     1801 gaccttggcg atgcgcggct tgccgacatg gacgcgagcg gcatcactat gcaggtgctt
     1861 tcggttgctg gccccggggc cgatctcgtc cctggccggg ctggtatcga tcttgcgcgc
     1921 gcctacaacg acgcgctcgc cgaggccatc gcgcgccatt ccacccgtta ccgcggcttc
     1981 gcccatctgc cgatgcttgc gccggacgcc gcgggggatg agctcgaacg cacggtcaag
     2041 aagctcggct tccatggcgc gctgatcaac ggtgccaccg atggaatgtt cctggacgaa
     2101 cccaggttcg ctccgattct gacccgtgcc gaggcgctcg gcgtaccgct ttacctgcat
     2161 ccgggcatcc cggcgccggc ggttcgcaac gcctattacg acaaccttcc gggcaacctc
     2221 agcttcacgc tcgcgctgtc ggcctggggc tggcattacg acaccgcggt ccatgtgctg
     2281 cgccttgcgc tcagcggcac gttggatcgg tatccgaagc tgaagatcat cgtcggtcac
     2341 atgggcgagg cgctgccctt catgatggac cggattgacg agaccacgtc ggcgcaggcg
     2401 aagtcgctgt tgaagcgatc ggtgcggcag atgatcgtag atcaggtgtg gatcaccacg
     2461 agcggcttct tcacgctggt gccattcatg gcagcgctga tgacattcgg cgtcgaccgg
     2521 ataatgttct cggtcgatta cccgtttgct tccaacacgc gggcccgtgc attcctcgat
     2581 gcgctgcctg tgtcaccggc ggaccgcgcc aggatcgcgc acggcaacgc ggacgaactg
     2641 ctgcggctgc cggtgtgatg cgctatcgat ccagccagat tgtcgcgagg tcctggttga
     2701 cgtagtggct cgggctgatc cggaagccct ttacgtagct gcggtaaggc acgatgcgat
     2761 agcgccacag taggaacgct tcgtgcgctt cggtatcgag gacgcgcttt tcgaattgcc
     2821 gcatcagcgc gcgctgtttt gagaaatccg tctcgagcag catccgatcg tagagtgaga
     2881 cttcctccgg atcgtcgtag ttgccgtaat tcgcgtcgga cacggagtgc ggcagatact
     2941 tagtgccgtc gagcagcgga ttgacgatgt tttggcattc tgcgtcgacc gccacggcga
     3001 aatcgccgtg gcgcagtgct tcctcgtacg ggccggtggg cagtacgcgc tgcgtcgcgt
     3061 ggatgccgat tttgctccac tcgtcgacta cccagatgcc gttgaatttg taaggctggt
     3121 ccacattccg gttcagtagc tcgaaggtca gaccatcggc gccggcctcg tgcaacagcc
     3181 ggcgcgcctc ggcgcgcgac ttctcgatat ctggccagta gccggcgatc tcctggagct
     3241 cggcatgcgt tgccgccagc ggcgagccag gaaacacgat gcctcccact gtccgggata
     3301 cggtgatccg cgacatcggt tccgcgccgt gccagcggtc gatcgccaag gtgagcgcac
     3361 ggcgcacgcg cgcatcgtcg aatggtttgc gcacatggtt cggcgtgatc agggcgccgc
     3421 agttccagtc tctctcctgc acggtgatct tgtcgcccag agccgcgaca agttcatcgc
     3481 gggtggccgg agggaatcca cggaactcga tcgctgcccg atcggatttg atcgctgcga
     3541 cgcgcacggc ctgcttgtcg gcgaagatgc cgatgaagcc atccagataa ggcaggcctg
     3601 gccgatagta atcgggactg cgcacaccgg aaatcgactg ccccaactga tagtccttga
     3661 accggaacgg tccggatccc aggatatgcg tctcgtacca atgcgggtcg cgatcgagaa
     3721 tggccttccg ataaatgaag gcatacggat cggcgagcgc aggcaggaag gcatcggtcg
     3781 agaacttcag ctggaacacg acggtctttg catccgctgc ctcgatcttg tcgatcatcg
     3841 catagtagcc acggcgcacg ctgatgacgc cttccggtgg attgacgatc ttgttccagc
     3901 ttgccgccac gtccagcgct gtcagcggcg cgccatcgct gaatttgata tcgtcacgga
     3961 tcaggaacgt atagcttttg ccgccgccag tgggctgcgg gatctccacg cagacgtcgc
     4021 aaacgaagtg agtcgtgtcg gccggattgt cgggattggc gcggatcagc acgctgtaga
     4081 acggcgccac cgcgtgaatg gtagcgaagg tgttctcgcg atggccgtcg aaactggggg
     4141 gtgcatccgc cgggatcata aaggtcagcg tgccgccatg cttcggcgtt tcatcggcga
     4201 ctgccggcac gccggacgcc aggacaccta tgcacattgc gatgaaccag tgacgcacgg
     4261 accgcctccc ttacatggcg agctgctcca gcggccaacg tggacgtggc gcaaagttca
     4321 tttcgttggt caggcccagg cgtagccgtt cgattcccgc ccaagccacc ataacggcat
     4381 tgtcggtgca caaccgcact ggcggcgcaa tcagctcgaa atcatgcgtc tcggctgccg
     4441 ttgccaatgc cgctcgtacc gcgccatttg ccgccacgcc accggccacg accagcgcac
     4501 ggacctccgg cgaccgctcc cgcatcatgg tcatcgcatg tgcggcacga tcggtcagta
     4561 cttcggccac tgcgtgctgg aagctggcgg cgatgtcggc tgccacggcg gcgggtaatg
     4621 cgccgctgcc caggcggccc acttccagtg ccaccgcggt cttcagacca gagaagctga
     4681 agtcgcaacc tgagcggcca agcaacggac ggggaaacgc gtagcgtctg gcatccccgc
     4741 cggccgccag cttctccagc gcgggtccgc ctggccagcc gaggcccagc aactttgcca
     4801 ccttgtcgaa ggcttcgccg gcagcgtcgt ccatcgtgct gcccagacgt acgtcatgcc
     4861 caacgccctc caccgcgacg cattggcagt gaccgccgga cacaagcagc aggagatacg
     4921 ggaacgccaa tccatccgga accagaccgg gcagacgcgc ggtcagcgca tgcgcctcca
     4981 ggtggttcac cgccacgtag ggcagccgat gcgccagcgc gatgcccttg gcgaactggc
     5041 tgccgacgat caggccgccg atcaatcctg gcccggcgct ggccgcaacg ccgccgaggt
     5101 cagcgaaccg caagccagcc tgcgacatga ctcggaccac ctgttctggc aggaaggcga
     5161 gatgggcgcg cgcggcgatc tccgggacaa caccaccata ccgggcgtgc tcgcactcct
     5221 ggctgagcac tgcctcggcc aggatgtgcc cgtccgcatc cagagcggct gacgcagttt
     5281 catcgcacga tgtttcaata ccgaggacag gccgcatgct gcccctttcc agttgtctga
     5341 gagagtaata ggacgcgccg atgatcaccg ccatgtcgcc actccgcgta ggaacgagag
     5401 gctcaccact ggcgctgatc cagacccgga tggtgatcga cgcgttgcgc ggcaggcggg
     5461 aggcagagcc cgcggtgatc cgcaccaccg gcgattcggt gcaggatcgg cggcttgccg
     5521 atatcggcgg gaagggcctg ttcgccaagg agatccacga tgcgctggcg cagggccgca
     5581 tcgacgcggc gatgcatagc ctgaaggatc tggaaacgga tctcccacaa gggatcgtgc
     5641 tcgcatgtgt cctgccgcgt gaggatgcgc gtgatgcgtt gatccttggc gccgcgggcc
     5701 ccgcagaccc agcacagcca ttcgccagcc tgccgcacgc ggcgcggatc ggtacagcct
     5761 cggtccgtcg ccaggcccag ttgctgcatg tgcggccgga tctcgccatg tccacgctgc
     5821 gcggcaacgt ggatacgcgt ctggccaaac ttgcggcggg cgagtgcgct gccacgctgc
     5881 tcgcctatgc cgggctgaag cggcttggcg tggcggatcg agccagcgcg atcctggagc
     5941 cggacatcat ggtcccggcg gccggccagg ggatcgttgg cgttacagtc agggaacagg
     6001 acgaagcgct gcgcgcgctg ctgagtgaca ttgcggaccc cgacgctatg gtggccgcca
     6061 cggcggaacg cgcgttgctc gcggagcttg atggctcgtg ccgcacgccc atcggcgctc
     6121 acgctgtatt gctgtccgat ggcgcgctcc gtctcctggc gatggtcgcg cgcgttgatg
     6181 gctccttcct gttgcggcgc agcctgcaag ggccagcatc ggatgcagtc cagctcggcc
     6241 gggaactggc cgcaagcatg cgtgccgaca gtccggacga tatctttgtc tgatcctggc
     6301 gccagcgtgc tgatcacccg acccgaaccg ggagcgagtg agacggcggc gcgcgttgca
     6361 gcgctgggat tgctgccggt ggtcgcacca ttgctgcaaa tccacacgtt gccgggtgcc
     6421 tggccgccat cggggcaggt gcaggcgtta gtcgtgacca gtggcaatgc gattggggcg
     6481 ctcccggcaa gtcatcgcca cctgctgctc ttggcggttg gccaggcaac tgcgcgccgc
     6541 gcgcgcgcag ccggattcga gcaggtcgag agcgccgatg gggacgccag cgccctggtc
     6601 gatctggcgg aacgggtttg ccaaaagcaa cgtgggcctt tgctgcttgc gaccggccgt
     6661 cgccagggca attcaattgc ggaggaactg cggcgccgag ggttcgctgc agtccgccgg
     6721 gttgtttatt ctgccgatcc ggttgcattg ctgccggcct ccgcgcgctc tgcgctacgg
     6781 tctggagcgc tccgcgccgc actgttcttt tcggccgaaa ccgcgcgagc cggtgtgcgt
     6841 ctgatcgaat cggcggcact gctcgacgcg gtccgcagcg tcgatgccgt ggctatcggc
     6901 aagcctgccg ccgtggcatt agaggcgctt ccgtggcggc gtgtccgcgt cgccgctcgg
     6961 cctgatcagg atgcgatgct ggcacttttg caatgagtga atcgtccact ttgccagccc
     7021 cggagccgga gccgacgccc gaaccaccag gcgcggcact gccggcgcgg cattccatac
     7081 tgccgtggtt gagcgctgcc ggatttgttg tgctcgctgg cgcgatcgta tgggtctggc
     7141 agaatccact gctgccgcct ccatccgcac aaaccggggc gctcgagcag caggtcgtgg
     7201 cgttggaggc tcgggtcgcc cgcctggaga agcagccccc aatcaccgca ccgaacctga
     7261 ccacgctgga agcgcgggtc gcagcattgg agcagcgtca acctgcggca gccgcgccgc
     7321 cgccgaatct cggtccgctg accgcccggt tggaggcact ggaacatcag caggctgcgg
     7381 atctggcgcc gatggaggtg cggatcagca ggctggagga cgccggccac caggtcctca
     7441 ctgacgtcac gcaaaagctc gcctccgagg atgcccggtt tgcggctctg gagaagagct
     7501 cgagccgggc ggtgaagata agcgctgcac gccaggctct ggatgcaggc cacaagcttg
     7561 gcgacgtgtc aggagcgccg cccgctttgg cgcgctatgc caccatcgat ccgcccaccg
     7621 aggcctcatt gcgccttgca ttcccggcca cgtcgcaggc ggctctggct gcgtcgcggc
     7681 cgtcggttga tggccagccg ttgctgtcac gtatttgggc gcgcgctcag gacttggtca
     7741 ccatccgccg gggcgaccag gtgctggtcg gtgatcctgc cgccggattg ctggccaccg
     7801 cgcggaccgc cctcgatgcc ggagatctgg ctggcgcggt gcgttcagtc ggagcgctga
     7861 ccgggccgcc ggctcaggca atggcgggct ggctcgctca ggcccgtgcg ctcctggaag
     7921 cgcgcgccgc tcttgccagc tgggccgcgc aaggctgatg cgccgtatat tgttcgccgt
     7981 cgtggcggcc gccgtggtgc tcgccgttgc ctgggcgctc gcggccatcc cgggccggat
     8041 gacagccaat atcggcgaca tcaccttcga ggccgcgacc tcggtcgtcg cgctcggact
     8101 gctgcttggg tttgtgctcc tctattcctt gttccgcctg ctcggtgctg ctgtgaggct
     8161 gccgcgctcg ctacgccggc gggcggcggc acggcggcgc aaggctggcg atgtcgccac
     8221 gacccgcacg ctgctcgcat tggcagccgg cgaaacggga gaggcgcgac gcgaggccca
     8281 tcgcgcgcgc cgactgcttg gcgatacacc cgcgacgctg ctgttggccg ctgaggccgg
     8341 ccgcatcgcc ggccgcactg acgaggccgt ggcggccttc cgcaccctgt cggagcgtga
     8401 tgacgcggct ttccttggac tgcgagggtt gcttcgcgac gcggtcgaac gggaagactg
     8461 gcaggaggcg gcggcacttg ctcgcaaagc cgaggccgtt cagccaggtg cggcctggct
     8521 ccggcgcgag cgcgcgcgac tagcagtgcg atccgcgaat tggtccgacg cgctggcgct
     8581 ggcagacgcg gacgcgccga aggcggcgct tgccactggt gccgccgatg ctgaggccga
     8641 tgtcatccgt gctacgcgtc tggcaaagga agcttggcag aatgactcgt cactggcgcc
     8701 agcggcgctg gcctatgcgc gccggctgcg tgcggcagga cgggagagca gagctctcgc
     8761 gacggtccgc cacagctgga gcatcgcgcc ccatcccgac ctcgccgcgt tcgcgctggc
     8821 tccgatcagc gatccgcttg cacgcatgca ggcggcgcgg cagctgaccg aagccaatcc
     8881 ggaacacgcg gaaagccgcc tgctgctggc acgcgcagct ttggatgcgg gcctcaccgg
     8941 cgaggcacgg cgctacgccg aggcggcccg cgcggccggc ctgaaccaac gacggctgtg
     9001 gctgctgctg gccgacatcg acgaggcgga gagcatcgac cagcgtgcgt cgctgcgcca
     9061 tgccgcgaca gccgaacctg atcccacctg gcgctgcggc gcctgccata cggcgcacgc
     9121 cgaatggcac ccggggtgtc ccagctgctt cactgttggc ggactgcact gggcctcgag
     9181 aggcccgatc gccgccccga tcctccaggc gcccgcttcg gaaacgctgc tgccgtaatc
     9241 aggactgcta gccgcgaacc tagcggtccc accgtcggtc gggagcccga gcctcttcca
     9301 gcggttccgg cgcgctccgc ggacaaggag cgagcgcgag gaacggcttc ggatcgaccg
     9361 ggtgaccgtc gatccgcact tcgaaatgca cgtgcgctcc atgcgcgcgg ccagtcatac
     9421 cgatctgccc gatcatatca ccctccgcga ccggcgcacc cggcgcgata ccaggcgcaa
     9481 actcagacat gtgggcgtat cgcgtgatca gcccgtcagc gtgacggata tcgacgatct
     9541 tcccgtaagc gaaataccat ccagcataca ccacgacgcc accggcggcg gcgcggatcg
     9601 gcgagccgta cggtgccatc aggtcgatcc ccgtgtgacc gtgaccgaag ccgcgggaga
     9661 tctcatgaag ctggccgggc ggcatcatca tcagagggca accggccccg cctgccggcg
     9721 ctgcgacggc cggggaggtg aggcacagtc cgatcacgag gagggcggcc gcacgcatcg
     9781 gtgcagggaa caggtatgtg agtggcgagt cgagtccgag accggaatga ccagaggacg
     9841 gcggccaatt ttcatgggtc gttggcaaag cctgctcccg ggctgttgtc gggcagtgcc
     9901 gcaaagtgcc gggtaattca tacaatttgg ttaagagcca acaggccacg ctgaacgcag
     9961 caagccggca acccgtcggt aagaattcag ttca
//
LOCUS       DATGEM010000148         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_766237, whole genome shotgun sequence.
ACCESSION   DATGEM010000148 DATGEM010000000
VERSION     DATGEM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766237"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1550)
                     /locus_tag="VKQ27_04635"
     CDS             complement(150..1550)
                     /locus_tag="VKQ27_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HLY88250.1"
                     /translation="MQHQLRLLAKATDGQLVREHPLPLVVLEFESPSAAIIATPVPAL
                     SRKTTLYVFLLVMSVLVASGLIRIDKIVSASGKLVAKTPNIVVQSFGESIVESIEVRE
                     GDVVREGQILARLNPTFATADLTTIKNQVDLLAAKAGRLQALTAGTDYAPDPSNPLAP
                     LQASIFKQQTAEHALSLQNYNQQIERLQTKIAGGESLIAYYRERVGIADKLEEMRQRL
                     EDLQYGTRVATLTAKDDRLNMAAKRDQAASEAAQASRELIALRADRDAYAQRWNGQNS
                     QELAETRSLLVQAQQAYEKASLNSHLRVLTAPRDAVVLSVGMISPGSIVTSGAPLLRL
                     VPIDAPLVAEIAIPGIDSGYVRPGETVRIKFDTLPFLQYGSALGVVRAISADSFSPET
                     APQEGGSLLPNRPRTLYYRGDVTLDTVQLHDTPQGFRLMPGMPLTADVKVGTRSVLAY
                     FIGKIIPIAQDSLHEP"
     gene            complement(1513..>2811)
                     /locus_tag="VKQ27_04640"
     CDS             complement(1513..>2811)
                     /locus_tag="VKQ27_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HLY88251.1"
                     /translation="LISAGCITLVIAAFLKPLAAMTAKLIQAESDKGSTLVESIYGIR
                     TVKSLCLENTRSAEWDQRIARVGELNLQMGRLSNWPMVLAMPFEKYTQAGVLAFGAFL
                     AVTSNNPLSLGSLIGFMMLGGRVAGPLVSLARLLQDWQEARQALAQIGWVLNRPTEKR
                     ALSHGVRPKFEGAISFEDVTFKYEGTKIPALSKVTFSIPPSTMLGVVGRSGSGKSTVA
                     RLLMGINRDYSGTVKIDGTDLREINLRHLRQSFGVVLQENFLFRGTVKDNILAGRPGL
                     SSEDVTWAARLAGAEEFIERMPQGYQTVIQEGSPNLSGGQRQRLAIARALIVDPTMMI
                     LDEATSALDPESEALINANLQRMALGRTMVIVSHRLSSLVDCDQILVLDRGQVIDVGP
                     HSQLVERCSVYRHLWLQQSRHVHQQGKLHAAPAPAIGQGD"
BASE COUNT          500 a          869 c          929 g          513 t
ORIGIN      
        1 ggcatcgccc gcctgtccgg ctgccgcgaa cagtgcaagc gcccggccgg gtgccgattc
       61 ggagagccgc gtcgcccgcg cccgcgccca tctcggagag cgggacgcga gcagatcgag
      121 cggactggcg aagagggtca atccggccat cagggttcat gcaggctgtc ttgggcgatc
      181 ggaatgatct ttccgatgaa gtaggcgagc acggaccgcg tgccgacctt cacatccgcg
      241 gtcaagggca tgccgggcat caggcggaag ccctgcggcg tgtcgtgcaa ctgtaccgtg
      301 tcgagcgtga catcgcccct gtagtaaaga gtgcgcggcc gatttggcag cagggagccg
      361 ccctcctggg gcgcggtctc agggctgaaa ctgtccgcac tgatcgcgcg caccacaccg
      421 agcgcgctgc catattgcag gaagggaaga gtgtcgaact tgatccggac cgtttcgccc
      481 ggccgaacat agccgctatc tatgccggga atggcaatct ccgccaccag cggcgcgtcg
      541 atgggcacga gccgaagcag cggcgccccg ctggtgacga tcgaaccggg ggatatcatg
      601 ccgaccgaca gcacgaccgc atcgcgcggc gcggtcagca ccctgagatg gctgttcaaa
      661 ctagcctttt cataggcctg ctgggcttgc accagcaggc tgcgcgtttc ggcgagctcc
      721 tgcgagttct gcccgttcca gcgctgcgca taggcgtcgc gatcggcacg cagggcgatc
      781 aattcgcggc tcgcctgggc ggcttccgaa gccgcctggt cgcgcttggc cgccatgttc
      841 agccgatcgt ccttggcggt cagggtcgcg accctggtgc cgtattgcag atcttcgagc
      901 cgctgacgca tctcctccag cttgtcggcg atgcccaccc gctcccgata gtaggcgatc
      961 agcgactctc caccggcaat cttggtctgc agcctctcga tctgctgatt gtagttctgc
     1021 agcgacaagg cgtgttccgc ggtctgttgc ttgaagatcg aggcctgcaa gggggcaagc
     1081 ggattggacg ggtcgggcgc gtagtccgtg ccggcggtca gcgcctgcag gcgcccggcc
     1141 ttggcggcga gcaggtcgac ctggttcttg atcgtcgtga gatcggcggt cgcgaaggtt
     1201 ggattgagcc gggcgaggat ctggccttcg cggacgacgt caccctccct cacctcgatg
     1261 ctctccacga tgctctcgcc aaaggactgg acgacgatat tgggcgtctt ggcgaccaac
     1321 ttgccgctgg ccgaaacgat cttgtcgata cggataagcc cgctggcgac gagcaccgac
     1381 atgaccagca ggaagacata gagagtggtc ttgcgcgaca gcgccggaac gggcgtggcg
     1441 atgatcgccg ccgacgggct ttcgaattcg aggacaacga gcggcagcgg gtgctcgcgc
     1501 accagttggc cgtcagtcgc cttggccaat agccggagct ggtgctgcat ggagttttcc
     1561 ttgttgatga acgtgtcggc tctgttgcag ccaaagatgg cgatagacgg agcagcgctc
     1621 gaccagttgg gaatgcggcc ccacatcgat gacctgaccg cggtcgagca cgaggatctg
     1681 atcgcaatcg acaagcgagg agagacggtg tgagacgatg accatcgtgc gcccgagcgc
     1741 catgcgctgg agattggcat tgatcagcgc ctcgctctcc ggatcgagcg ccgaggtggc
     1801 ctcatcgagg atcatcatcg tggggtcgac gataagggcg cgcgcaatgg cgaggcgctg
     1861 acgctggccg ccggagaggt ttggtgaacc ttcctggatc acggtctggt agccctgcgg
     1921 catacgctcg atgaactctt cggctccggc aagacgggcc gcccacgtca cgtcctcgga
     1981 actgaggccg ggtcgtccgg cgagaatgtt gtcctttacc gtgccgcgga acaagaagtt
     2041 ctcctgcagt accacgccga aactctggcg caggtgcctc agattgatct cgcgcagatc
     2101 cgtcccgtcg atcttcaccg tccccgaata gtcgcggttg atgcccatga gcaggcgggc
     2161 gacggtggat ttgccggagc ccgaccggcc gaccaccccg agcatcgtac tcggcggaat
     2221 ggaaaacgtg accttgctca gcgccggaat cttcgtgccc tcatatttga aggtcacatc
     2281 ctcgaaactg atcgcgccct cgaatttggg gcgtacgccg tggctcagcg cgcgcttctc
     2341 ggtcggtcgg ttgagcaccc acccgatctg ggcaagggct tggcgcgctt cttgccagtc
     2401 ctgcaagaga cgcgcgagac tcaccagcgg gccagcgacg cgaccgccca gcatcatgaa
     2461 gccgatcaaa ctgccgagag acagggggtt gttggaagtc accgcgagaa acgcgccgaa
     2521 cgccagcacg ccggcctggg tatatttttc gaacggcatc gccagcacca tgggccagtt
     2581 gctgagcctg cccatctgca ggttgagctc gcctactctc gcgatccgtt gatcccactc
     2641 cgcggagcgg gtgttttcga gacagagcga cttcacggtg cggatgccgt agatgctctc
     2701 gaccagggtc gagcctttgt ccgattcggc ttggatcagc ttggcggtca tcgccgcgag
     2761 cggcttgagg aacgctgcga tgacgagcgt gatgcagccg gcggagatca g
//
LOCUS       DATGEM010000149         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1670790, whole genome shotgun sequence.
ACCESSION   DATGEM010000149 DATGEM010000000
VERSION     DATGEM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670790"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1013)
                     /locus_tag="VKQ27_04645"
     CDS             complement(267..1013)
                     /locus_tag="VKQ27_04645"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY88252.1"
                     /translation="MTQQVVLITGALAGIGRATAIAFARAGARLIVSGRHDEAGTAFA
                     SELRDLGTEAEFAHADTRHDDEVRSLVERAVARFGRLDVAVNNAGTEGQPRLLVDETP
                     EACATVFDTNVLGVVLSMKYELRVMQGQKAGCIINLSSVAGHKGLPGVSIYVASKHAV
                     EGLTKVAALEAAPFGVRVNAVGPGPIATDMLDRFTGDAAGKAAFLSGVPLKRAGTPEE
                     VAQTIVFLASEQASFITGQVLSIDGGISAQ"
     gene            complement(1164..1727)
                     /locus_tag="VKQ27_04650"
     CDS             complement(1164..1727)
                     /locus_tag="VKQ27_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88253.1"
                     /translation="MIGTRSALLVASIFVAGPAIAATPSDGHYVWVPAGEAVVVAPKA
                     PTRPIAFPVVDMLARQEAMMQRIMADMDTMMAIPLPNPDQIMQAAMQGMPQLAPGSSV
                     VVTSISIPGGTCSQTITYGATSQGVQPVVKVVSSGNACGAIQANGPISVVQSTPIPQP
                     AAQQPQQRLWTIGYPQHPITTGVRPRT"
     gene            complement(1811..2443)
                     /locus_tag="VKQ27_04655"
     CDS             complement(1811..2443)
                     /locus_tag="VKQ27_04655"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLY88254.1"
                     /translation="MIHLDYAALRATPVATDPFPHVVVPYFVPPDTLWAVLADMPPLD
                     KRGSFPIDALSLGPNAQALMDAMEGPLLREAIADRFGLDLSDAPTMVTLRGWTNDRDG
                     YIHVDSTAKRVTVLLYLNQAADAFTQHDGCLRLLRGPDNLDDFAVEVPPVNGTLLVFP
                     NAANAWHGHKRFTGQRYSVQLNYMTTDAKARSELRRHRISAFMKRLTRAA"
     gene            2514..3656
                     /gene="zapE"
                     /locus_tag="VKQ27_04660"
     CDS             2514..3656
                     /gene="zapE"
                     /locus_tag="VKQ27_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HLY88255.1"
                     /translation="MDNPGADIAAQRGPLPAFRALVAAGELAPDPAQERVARRLHGLW
                     EQLHGYDPGPRPAAGLFAWLRRQQQIEAPDGLYIVGAVGRGKSMLMDLFFSAADVRRK
                     QRIHFHRFMQNVHLRFHAFKRAHPEIEDPIPPLADTIATEAALLCFDEFQVNDIADAM
                     ILGRLFQALFERGVVVVATSNIAPDDLFKGQPGRDAFLPFIALIKQRLDVVMMDDGRD
                     FRRERLRGMRTWLVPADARADREMDRAFAELTGGAAGKSDALWVMGRKFLVPLAAEGV
                     ARFDFDTLCGAALGPGDYLALATRYHTLILDGVPRLSPDNYDKARRFITLIDTLYDHR
                     VKLLASAEALPDQLYQRGDNAKMFERTASRLDEMQSADWLELEHLP"
     gene            complement(3701..>4592)
                     /locus_tag="VKQ27_04665"
     CDS             complement(3701..>4592)
                     /locus_tag="VKQ27_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY88256.1"
                     /translation="KARDLLAQDDAAHERQLAQDAREVSRLRRNAHELKNVGINSGSD
                     LLLKKSKQLRERAETLEQSLVAQHVERNGDIRLSNRGTHAKVLLSLANVDVRAPDGRK
                     LFHIAKLDVFQQDRIVLLGRNGVGKSQLAQLLRRALAGDEVAGVRASPSIVTGYVDQL
                     MSHLPDASTPHAFISGTFRPGDQRTISLLASAGFPVETQRRLIGSLSPGQKARLGLLA
                     LRLTEPNFYLMDEPTNHVDIAGQERLEAEILAHEATCIIVSHDRAFVQGIATRHLLIE
                     NGMISEVDTPETFYRGLRED"
BASE COUNT          787 a         1464 c         1497 g          844 t
ORIGIN      
        1 ttcggcgcga ccggcctatc tgggcgtggc ttggcggtgc cgacatggcg gaagccgatg
       61 atctcggtcc agaagcaatg cgattcttcc atgtcgcgca cgttcagcac gacgtggttc
      121 agcgccatcg gggtctcggg gcgatcgggg atattcatgt ccatgggttg gctcgtgtcc
      181 tgcacggcga accctaggca tgcaccctcg atgggcgtcc ttgacctgcg tcaaacgtgt
      241 ctgaaccgga ccgaacgcgg tggcccctac tgcgcggaaa tgccgccgtc gatcgacagg
      301 acttgcccgg tgatgaatga ggcctgctct gaggcaagga atacgatggt ttgcgcgacc
      361 tcttccggtg tgcctgcacg cttgagtggc acgcctgaca ggaacgcggc cttgcctgcg
      421 gcatctccgg tgaagcgatc cagcatgtcg gtcgcaatcg gtcccggacc cacggcgttg
      481 acgcggacgc caaacggcgc cgcttccagt gcggccacct ttgtcagccc ctcaacggca
      541 tgcttgctcg cgacatagat cgacactccc ggcagtccct tgtgcccggc aaccgaggac
      601 aggttgatga tgcagcctgc cttctgaccc tgcatcaccc gcaactcgta cttcatgctc
      661 agcacgacgc ccagcacatt ggtgtcgaac acggtagcgc aagcctccgg cgtctcatca
      721 accagcagcc tcggctggcc ttcggttcct gcattgttga ccgcgacatc cagtcggccg
      781 aaacgcgcaa ccgcccgctc aaccagagag cgcacctcat cgtcgtgacg tgtgtccgca
      841 tgcgcgaatt ccgcttcggt gcccagatcg cgtagctcgc tggcgaatgc ggtccctgct
      901 tcgtcgtgac gaccagaaac gatcaatctg gcaccagccc gcgcgaaagc aatcgctgtg
      961 gcgcgcccaa tgccagcgag cgccccagtg atgagaacta cctgctgggt catgaaatgt
     1021 cctcccgccg gctggaaatg gaggactacc ttggcggcag gagcagcgct gcgattgtca
     1081 gatcacgcag attctgaaga tttctgcgga tgcgcctgac gaaatctggc cggcacccgg
     1141 cacctagcgg ccggaccacg agatcaggtc cgcggtcgaa cgccagtcgt gattgggtgc
     1201 tgcgggtagc cgatagtcca caggcgctgc tgtggctgct gtgcggcagg ttgaggaatg
     1261 ggggtggatt ggaccacgct gatcgggccg ttcgcctgga tggcgccaca cgcgttgccg
     1321 ctgctcacga ccttcaccac cggctggacg ccctgggagg ttgctccata ggtgatggtc
     1381 tggctgcagg tgccgccggg aatggaaatc gaggtcacca ccacgctgga gccaggggcc
     1441 agctgcggca tgccttgcat ggcggcctgc atgatctgat cggggttcgg caggggtatg
     1501 gccatcatcg tgtccatgtc ggccattatc cgctgcatca tcgcctcctg gcgtgcgagc
     1561 atgtcgacaa ccgggaaggc gatcgggcgc gtcggggctt tcggcgccac cacaacggct
     1621 tcgcctgccg ggacccacac gtaatgccca tctgatggtg tcgcagcgat ggcggggccg
     1681 gcgacgaaga tgctggcaac aagcaaggca ctgcgcgttc cgatcatgag ctcctccact
     1741 tgcaagctga gacatgattc tcctacccgg cttgccgcgc cttttcccgg cggatgaagc
     1801 tttcgtaagg tcaggccgcg cgggtgagac gcttcatgaa agccgaaatg cggtgccggc
     1861 gcagttcgct gcgggccttg gcgtccgtgg tcatgtagtt gagctgcacc gaatagcgct
     1921 gtccggtgaa acgcttatgg ccatgccagg cgttcgctgc gttggggaac accaggaggg
     1981 tgccgttcac cggcggcact tccacggcga aatcatccag attgtccggc ccgcggagca
     2041 ggcgcaggca cccgtcgtgc tgggtgaacg catccgccgc ctggttcaga tacaacagca
     2101 cggtgacccg cttggcggtc gagtccacat ggatgtagcc gtcacggtca ttggtccagc
     2161 cgcgcagcgt caccatggtg ggcgcatccg acagatccag cccgaatcgg tcggctatcg
     2221 cctcccgcag caacgggccc tccatcgcat ccatcagcgc ctgggcgttt ggcccgagcg
     2281 agagcgcgtc gatcgggaag gaaccgcgct tgtccagtgg cggcatgtcc gccagcaccg
     2341 cccacagggt gtcgggcggg acgaaatacg ggacgacgac atgggggaag ggatcggtgg
     2401 cgaccggggt ggcgcgcagc gccgcatagt cgagatggat catgacggcg ctgcttagca
     2461 cagccggcgg tgcgttgaac cccacccccg gacacgctat ctgcgaggcc gatatggaca
     2521 acccgggcgc cgacatcgct gcgcagcgcg ggccgctgcc agcctttcgg gcgctggtag
     2581 cggctggcga actcgcgccc gatcccgcac aggagcgcgt cgccaggcgc ctgcatggcc
     2641 tgtgggagca attgcatggc tacgaccccg ggcctcggcc agcagccggg ctgttcgcct
     2701 ggctgcggcg tcagcagcaa atagaggcgc cggacggcct ctacatcgtc ggggccgtcg
     2761 gacgcggcaa gtcgatgctg atggacctgt tcttctcggc ggcggatgtg cgcaggaagc
     2821 agcgcatcca cttccatcgc ttcatgcaga acgtccatct gcgcttccac gcgttcaagc
     2881 gggcccatcc ggagatcgag gatccgatcc cgccgctggc tgacacgatc gccactgaag
     2941 ccgcactgct gtgcttcgat gaattccagg tgaacgacat agccgatgcc atgatcctgg
     3001 gccggctgtt ccaggcgctg ttcgagcgag gcgtcgttgt ggtggcgacc tccaacatcg
     3061 cgccggacga cctgttcaag ggccagccgg ggcgcgatgc gttcctgccg ttcatcgcgc
     3121 tgatcaagca gcggttggac gtggtgatga tggatgacgg ccgcgacttc cggcgcgagc
     3181 ggctgcgcgg catgcgcacc tggctggtgc cggcggatgc gcgcgccgat cgcgaaatgg
     3241 atcgggcatt cgccgagctg accggcggtg ccgccggcaa gtccgatgcg ctgtgggtga
     3301 tggggcggaa gttcctcgtg ccattggctg ccgagggagt ggcgcggttc gacttcgaca
     3361 cgctgtgcgg cgcggcattg ggcccgggcg actatctggc gctggcgacg cgctatcaca
     3421 ccctgatcct tgatggcgtg ccgcgactgt cgccggacaa ctacgacaag gccaggcgtt
     3481 tcatcacgtt gatcgacaca ttgtatgatc atcgcgtgaa gctgctcgcc tcggccgagg
     3541 cgttgccgga ccaactttat cagcgtggcg acaacgcgaa gatgttcgag cggacggcat
     3601 cgcgcctgga tgagatgcag agcgcggact ggctggagct cgagcacctg ccatagtaca
     3661 ttgaggatcc gccacgctcg cgccagtgcg atccagccga tcaatcctcc ctgaggccgc
     3721 gataaaacgt ttcgggcgta tctacctccg agatcatccc gttctcgatc agcagatgcc
     3781 gcgtggcgat cccctgcacg aaggcgcggt cgtgcgagac gatgatgcag gtcgcctcat
     3841 gtgccaggat ctccgcctcc agccgttcct ggccggcgat gtcgacgtgg ttcgtcggct
     3901 catccatcag gtagaaattc ggctccgtga gccgcagtgc cagcagacca agacgcgcct
     3961 tttgcccagg cgacaggctg ccgatgagcc tccgctgcgt ctccacggga aaaccggcgc
     4021 tggccagaag gctgatggtg cgctgatcgc cagggcggaa tgtgccgctg atgaacgcgt
     4081 gtggcgtgct cgcatccgga agatgcgaca tcagttgatc gacatagcca gtcacgatgg
     4141 atgggctggc gcggacgccc gcaacttcat cgccggcgag tgcccggcgc agcagttgcg
     4201 ccagttgcga cttgcccacg ccgttgcggc ccagcagcac gatgcgatcc tgctggaaca
     4261 cgtcgagctt ggcgatgtgg aacaacttgc gcccatctgg tgcccgcacg tcgacgttgg
     4321 ccaacgacag cagcactttc gcatgtgtgc cccgattgct gaggcgtatg tccccgttgc
     4381 gttccacatg ctgcgcgacc agcgactgct ccagtgtctc ggcccgctcg cgaagctgct
     4441 tcgacttctt cagcagcaga tcgctgccgc tgttgatgcc gacattcttc agctcatggg
     4501 cattgcggcg caggcgcgat acttcgcgcg cgtcctgcgc aagctgccgc tcatgcgccg
     4561 cgtcgtcctg tgccagcaga tcccgcgctt tt
//
LOCUS       DATGEM010000150         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1325220, whole genome shotgun sequence.
ACCESSION   DATGEM010000150 DATGEM010000000
VERSION     DATGEM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325220"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VKQ27_04670"
     CDS             complement(<1..545)
                     /locus_tag="VKQ27_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HLY88257.1"
                     /translation="MRFVRPVGRVLKAAEAGLYRDASAAVAIAVATASRQRAELAVEL
                     VRERARIREEAAAEARAEATRLLADTALATQRAAAGMTRDIAEAIAAGVAIVINGRGL
                     AEAVARAAERAVSELSERNAVTVRVPPDALLATRERLEATAPAVRVVADATLPADGCI
                     VETNAGFVRAGLSEQLANLETA"
     gene            complement(545..1114)
                     /locus_tag="VKQ27_04675"
     CDS             complement(545..1114)
                     /locus_tag="VKQ27_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88258.1"
                     /translation="MQHFVRDIALWAEPALATPLLAAGLRAETAARLLRHPRFAARAS
                     TALVERLPLADADILEPQDYDAALSDAATLTRIAARAGAVWHAHRLRNLLLARDIAPL
                     VERFGEDLRAIALRHAALAPQDTADDEFAGAIDRDGRRCVATWIDALPAWAAARVRLK
                     WCSAWPAFEVPLRPAAVSIVRAVSAEAGS"
     gene            complement(1123..1902)
                     /gene="sctJ"
                     /locus_tag="VKQ27_04680"
     CDS             complement(1123..1902)
                     /gene="sctJ"
                     /locus_tag="VKQ27_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HLY88259.1"
                     /translation="MIRRPACLLLLLSLALLAGCKTQLDSGLSEREANEEVALLLRSG
                     IPASREADPKTSQVTVWVEQSRFADAVDLLGAHGLPRQHYDSIADVFRSGGLIASPTE
                     DRARMVYALSEELSHTISEIGGVLAARVQIVLPDNDPLRQDQPPSSASVFIRYRPGSE
                     VVNMVPQVKMLVANGVAGLSYDKVEVTLDRAAVANDLAPQLPAMVNFLGIWVEAGSVR
                     MLQMIAAGAGSLILLLSAAAAWFVWRGQGGERRAGANLTLR"
     gene            complement(1899..2324)
                     /locus_tag="VKQ27_04685"
     CDS             complement(1899..2324)
                     /locus_tag="VKQ27_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88260.1"
                     /translation="MAALPPNPPPIEPPQAEPVTSQPMQVDAFQSAMANAGTVLFANP
                     DAVGRALMAELDGFNIRATQVQQAVGASLADPDPAPANNATQPVTSDVGHALDVMHQH
                     QEHMLGLMMETYSFALEAEAVSRAATTFTSSVNTLIKTQ"
     gene            complement(2384..3451)
                     /locus_tag="VKQ27_04690"
     CDS             complement(2384..3451)
                     /locus_tag="VKQ27_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HLY88261.1"
                     /translation="MNDMMPALNMSTDFSSTAMTPFYSSYAGMSDGQLEGLAQLQSSY
                     SPSAPMANQHEALSLLQAAMQATDPALKNALLQQALALLTPGGGSATPAAGGGAPAVG
                     GGALASGPADPSSFSAPSAGTSSDSTPLTVDSANNSINTGRYTISGSNNDDGSLTITD
                     NQTGKTVEVWGDPHIKVNGQNVADFQKDGLTIQLQDGTQVHIQRTALSNGVSHLNQVS
                     VTKGDQAVTMSGFTTGQMHTSDVMNGAAAYQNGLYNAPDEINITEGDDGNLYYNNANG
                     SMGSEITAQSNGGQTDLDGAGGGLIGQAGVSTQAGASGNGQGLMAQLAGSIQSYSSGI
                     YQMEMMSILSELQSNSSFMQA"
     gene            complement(3496..3693)
                     /locus_tag="VKQ27_04695"
     CDS             complement(3496..3693)
                     /locus_tag="VKQ27_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88262.1"
                     /translation="MDLLGGIEGAVGGILGDMTGGGDPMAFVGELLNAIDGGNQNGGS
                     GGGSGMSGPIQDVAPLALAFL"
     gene            complement(3872..4393)
                     /locus_tag="VKQ27_04700"
     CDS             complement(3872..4393)
                     /locus_tag="VKQ27_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88263.1"
                     /translation="MMAAVAGASVGQAAALPHGDHVLQYSVVDQDLRDAISGIGAELG
                     LRVDISNAVHGHVHGRLPSGTASEVLNRLGAVYGFQWVCENNTLYVSANSELTTKILE
                     ISAVDPAMFTRALQDFHLVDDRWPVRIATDSGLAMIEGPPPYVAMIDQVLIALEKRPP
                     VEVHVFHGPAAKP"
     gene            4925..5086
                     /locus_tag="VKQ27_04705"
     CDS             4925..5086
                     /locus_tag="VKQ27_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88264.1"
                     /translation="MSGTTTDPMAAMQQAESDMQSTYAQAIGEEQKITDEKTLFDVAL
                     DAAKQRPQI"
     gene            5256..6140
                     /locus_tag="VKQ27_04710"
     CDS             5256..6140
                     /locus_tag="VKQ27_04710"
                     /inference="COORDINATES: protein motif:HMM:NF028003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HLY88265.1"
                     /translation="MPLASPPSTPKADPAWSCQVISGPNAGAMVRLHPGRYRLGSALA
                     NDIVLADTSIAAEQAVIEISNNHAAIELLADGTSLGRRKLRGGSRRALKPRSELTLGA
                     SRLRLTAPQQGGRQPLMLALPLLIGVACVALGLAPAASKHPTPAPAAIQPRQVQIAAA
                     RPEAADDAIAALRVRLTADGLGAALHVEEEQGAVMVAGSILATDRERWQAEHAWFDGR
                     FGADIALLDRVTFSTTQDLPRLDVRAVAMSPVPYVITASGDRYMAGAVLQGGWKITRI
                     ALNCVVLQRNGHEIDVTL"
     gene            6142..>6524
                     /locus_tag="VKQ27_04715"
     CDS             6142..>6524
                     /locus_tag="VKQ27_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88266.1"
                     /translation="MAAYPIDHTPAATPIDPARRVPPTRRVRHAGDLGPAEQAEQLDP
                     IEALAALTNEPGGIGSNGLADQLLDYVRPRPRHPDALTQPRIIPLLGAAADLLRNAAS
                     QEDTIVALGGTALEQELRQHRMLAER"
BASE COUNT         1181 a         2100 c         2080 g         1163 t
ORIGIN      
        1 gcggtctcga ggttggccag ttgctccgac agcccggcgc gcacgaaccc ggcgttcgtc
       61 tcgacgatgc agccgtcagc aggcagcgtg gcgtcagcga cgacacgcac ggctggcgct
      121 gtcgcttcga gccgttcccg cgttgcaagg agtgcgtccg ggggcacacg caccgtcacc
      181 gcattccgct cagaaagctc gctgacggcc cgctcggcag cgcgcgccac agcttccgcc
      241 aatccgcgtc cgttgatcac gatcgcgacg ccggcggcga tcgcctcggc gatatcccgg
      301 gtcatgccgg ctgctgctcg ctgggtggcg agagccgtgt ccgccagcaa gcgcgttgcc
      361 tcagcgcgcg cctccgccgc agcctcttcc cggatacggg cccgttcgcg aaccagttcc
      421 actgccagtt cggcgcgctg gcgggaagcc gtggcgacgg cgattgcgac cgcggcgctt
      481 gcatcgcggt agaggcctgc ctcagccgcc ttgagcacac gaccgacagg gcgaacgaag
      541 cgcatcacga acccgcctca gccgagacgg cgcgcacgat cgacacagct gccggacgca
      601 acggcacttc gaaggccggc cacgcgctgc accacttcaa gcggacacgc gcggcagccc
      661 aggccggcag ggcatcgatc caggtcgcaa cacagcgccg gccatcgcga tcgatcgcac
      721 cagcgaattc atcgtccgcc gtgtcctggg gtgccagcgc ggcatgccgc aaagcgatcg
      781 cacgcaagtc ctcgccaaag cgctccacca gcggcgcgat atcgcgggcg agcagcaggt
      841 tgcgcagccg atgcgcgtgc cacacggcgc cggccctggc agcgatgcgc gtcaacgttg
      901 cggcgtccga cagggcagcg tcgtagtcct gcggttccag gatgtccgcg tcagcgagcg
      961 ggaggcgctc aaccagtgcc gtggacgcac gcgcggcaaa ccgcggatgg cgcaatagac
     1021 gtgcggcggt ctcggcccgg aggcccgccg ccagcagcgg agttgccagc gccggttctg
     1081 cccagagggc aatgtcgcgc acgaagtgct gcattgcgcc gctcatcgca gggtgaggtt
     1141 ggcgcccgca cggcgctcgc ccccctggcc gcgccagaca aaccatgcgg ccgccgcgct
     1201 cagcagcagg atcagcgagc ccgcgccggc ggcgatcatc tggagcatgc gtacgctgcc
     1261 cgcttcgacc cagatgccga ggaagttcac catcgcagga agctgcggcg ccagatcgtt
     1321 tgcgaccgca gcgcgatcca gcgtcacttc caccttgtcg tatgacaggc cggcaacgcc
     1381 gttcgccacc agcatcttca cctgcgggac catgttgacc acttccgagc ccggccgata
     1441 gcggatgaac accgaggccg acgagggcgg ctggtcctga cgcagcggat cgttgtcggg
     1501 caggacaatc tgcacgcgtg ccgcgagcac gccaccgatc tcggatatcg tatgcgacag
     1561 ctcctcgctc agcgcataca ccatgcgggc acggtcttca gtcggagagg cgatcagccc
     1621 acctgacctg aacacatcgg cgatcgaatc atagtgctgc cgcggcagac cgtgcgcgcc
     1681 gagcagatcg accgcatcgg cgaagcggct ctgttccacc cagacggtga cctgactggt
     1741 ctttgggtct gcctcacgcg atgcgggaat gccgctgcgc aataatagcg cgacctcttc
     1801 gttggcctcg cgctcggaca ggccggaatc gagctgcgtc ttgcatccgg cgagcagtgc
     1861 aagagacagc aagagcagga ggcaggcagg acgtcgtatc attgcgtctt gatgagtgta
     1921 ttgactgagc tggtgaacgt ggtggccgca cgtgagacag cctcagcctc cagggcgaaa
     1981 ctgtaggttt ccatcatcag tccgagcatg tgctcctggt gttgatgcat cacatcgagc
     2041 gcatgcccta cgtccgacgt aacgggctgc gtcgcgttat tggctggtgc aggatccggg
     2101 tcagccaggc tcgcgccgac tgcctgctgc acctgggtcg cacggatgtt gaagccatcg
     2161 agctccgcca tcagcgcgcg acccaccgca tcgggattgg cgaagagcac cgtacctgcg
     2221 ttggccatgg ccgattggaa agcatcgacc tgcatcggct ggctggtcac gggttcggcc
     2281 tgcggcggct cgatcggtgg cggattggga ggcagagcag ccatgaatcc tcctcgtcgc
     2341 gaaaatgacg ggccggccgc agcaagtcgg ccggcccgct cgatcaggcc tgcatgaagc
     2401 tcgagttgga ctgcagttcg gacaggatgc tcatcatctc catctgatag atcccagagg
     2461 agtagctctg gatagagcct gccagctgcg ccatcaggcc ctgaccattg ccggacgcac
     2521 cggcctgcgt ggagacgcca gcttggccaa tgagtccgcc accggcacca tccagatcgg
     2581 tctggccgcc attggactgg gcagtgatct cgctacccat gctgccgttg gcgttgttgt
     2641 agtacaggtt gccgtcgtca ccctcggtga tgttgatctc atcgggggcg ttgtagaggc
     2701 cattctgata cgccgcggcg ccattcatga cgtccgaggt gtgcatctgt cccgtcgtga
     2761 agccgctcat cgtcacggcc tgatcaccct tggttacaga cacctggttg aggtgcgata
     2821 cgccgttgct gagcgcggtc cgctggatgt gcacctgggt gccatcctgt agctgaatcg
     2881 tcagtccatc cttctggaag tcggcaacat tctggccatt cactttgata tgcggatcgc
     2941 cccacacttc aaccgtcttg ccggtctggt tgtcggtgat ggtcagcgaa ccgtcgtcgt
     3001 tgttgctgcc actgatcgtg taacggccgg tgttgatcga gttgtttgcc gagtcgacgg
     3061 tcagcggagt gctgtcagag gaagtaccag ccgatggagc gctgaagctg ctggggtcag
     3121 cgggaccgct ggcgagcgca ccgccgccga ccgccggcgc accgccccca gccgctggcg
     3181 ttgcggagcc gccacctggc gtcagcaggg caagcgcctg ctgcaacagg gcgttcttca
     3241 gcgccgggtc ggtcgcctgc atggctgcct gcagcaggct cagcgcctcg tgctggttcg
     3301 ccataggggc agatggagag tagctggact gaagctgcgc caatccttcc agctgaccat
     3361 ccgacatccc ggcatagctg gaatagaaag gcgtcatggc agtactcgag aagtccgtgc
     3421 tcatgttgag cgcgggcatc atgtcattca tcgaactgtc cttcggttat cggctgcgac
     3481 ggatgctggc caatgtcaga ggaaggcgag cgcaagcggt gcgacatcct gaatggggcc
     3541 gctcattccg ctgccaccgc cactaccgcc gttctgattg ccgccgtcga tcgcattgag
     3601 cagctcgcca acgaatgcca tcggatcgcc cccacctgtc atgtcaccga ggatgccgcc
     3661 gacggcgccc tcaattcctc ctagcaagtc catatgggtt gtctcctgct tgagattcga
     3721 tggcgactga cgggcggaca gtcgccgggg cacacgacct gcagccggta gacgctcatg
     3781 ctgcaagcca aacaacttac ctgccgcgcc ctgcggaatt ggctcggatg ccgggtagcg
     3841 aactgcgaca ggatggcgat cattgtccgc atcacggttt tgctgcggga ccgtgaaaca
     3901 cgtggacctc gaccggtggc cgcttttcca atgcgatcag cacctggtcg atcatggcga
     3961 cataaggcgg tggcccctcg atcatggcca acccgctatc cgttgcgatc cgcacgggcc
     4021 atctgtcgtc aaccagatgg aagtcctgca gtgcgcgcgt gaacatcgcg ggatccaccg
     4081 cactgatctc caggatcttg gtggtcagct cgctgttggc tgaaacatac agcgtgttgt
     4141 tctcacagac ccattgaaag ccgtacaccg cgccaagccg attcagtacc tcagacgcgg
     4201 taccggacgg cagtctgccg tggacatgtc catgcacagc attgctgata tcgacgcgca
     4261 ggccgagttc agcgccaatg ccgctgatgg catcgcgcaa atcctgatcc accacgctgt
     4321 actgcaggac atgatcgcca tgcggcagtg cggctgcctg gccaacagat gcgcccgcaa
     4381 cggcggccat catcagtgca attgtcgaat gtataacgcg ggcacgaaac ggaatcgggc
     4441 gtctgggcac gccggcattc acgtctccct gcgccatttt catttcgaga tcctcaagtt
     4501 cccgacctgc atagttctga acctcaattg gtgcattgga caattcaacg gcgcgtgtgt
     4561 gaagcggagt ctgcgtgtat atttggtggc agaattgtgt ccgagcattc gtgatcgtcg
     4621 atgatgaaag aatttttcag gcatggttga cagccgacct gccgctaagg gagtcaatgg
     4681 cgtgtcatgc ccggccatga ttgcgatggg tgggagggga aaaggatcgt ggtgtgatct
     4741 gtagatttgc cgcgactcat ccgaatggcg cgatgcaaag cctatcgccg gtcgtatgtg
     4801 tttggccagt ccgcaagcta ccagccacgc cttcttgccc gactttccgt cgatcagcgc
     4861 ccgcgtgccg ggacgcgaag ccatcgcgct tcggggcaac caacctatag cggagactat
     4921 actcatgagc ggtacaacaa ctgatccgat ggcagccatg cagcaggccg agagcgatat
     4981 gcagtcaacg tacgcccagg cgatcggcga ggagcagaag atcaccgacg agaagacgct
     5041 gttcgacgtg gcactcgacg ccgcgaagca gcgtccgcaa atctgatctg accggatcag
     5101 ccttcgccta agggacccgc gctcggtctc ggggattctg gtcgggcggc cggaatcccc
     5161 tgcgggtttt gtatgtccat cggccgccgg ctgtcagcat cttccgggat acccgggtgt
     5221 cgacgtagcc acacatgtct acatggagca ccgtcgtgcc gcttgcatcg cctccctcga
     5281 ccccaaaagc agaccctgcg tggtcgtgcc aggtcatcag cgggcccaac gccggcgcga
     5341 tggtacggct gcaccccggc cgctatcgcc tcggctctgc gctggccaac gacattgtgc
     5401 tggccgatac cagcattgca gccgagcagg cggtgattga gatatcgaac aaccacgccg
     5461 cgattgagtt gttggccgac gggacgagcc tcggtcgccg caagctgcgc ggcggttcgc
     5521 ggcgagcgct gaaaccccgc agcgagctga cactcggcgc atcgcgcctg cgccttaccg
     5581 ctccccagca gggagggcgg caaccgttga tgctggcact tccgctgctt atcggcgtgg
     5641 catgtgtggc gctggggctt gccccggctg cctcgaaaca tccgacgcct gctccagcag
     5701 ccattcagcc gcgacaggtg caaatcgctg ccgctcggcc ggaggccgcc gatgatgcga
     5761 tcgctgcact gcgcgtgcgg cttacggccg atggtctggg tgcggcgctc catgtcgaag
     5821 aggagcaggg agcagtcatg gtggcaggca gcatcctcgc gaccgatcgc gagcgctggc
     5881 aagcagagca cgcgtggttc gatggcaggt tcggagccga catcgcactg ctcgatcggg
     5941 tgacattctc gaccacgcag gatctgccgc ggctggacgt ccgtgccgtg gcgatgtcgc
     6001 cggtgcccta tgtgatcacg gcgtcgggtg accgctacat ggcaggtgcg gtgctgcaag
     6061 gcggatggaa gataacccgc atcgccttga actgcgtcgt gctgcaacgc aatggccacg
     6121 aaatcgatgt gacgctctga catggccgca tatccaattg accatacacc cgctgcaact
     6181 cccatcgatc cggcgcggcg cgttccgccg acacggcgcg tccgtcacgc cggcgacctg
     6241 gggccggcgg agcaggccga gcaactcgac ccgatcgagg cgcttgcggc gctgacgaac
     6301 gaaccgggcg gcatcggatc gaatggattg gcagaccagt tgctggatta tgtcaggcca
     6361 cgtccgcgcc atccggacgc gctgacacag ccacggatca tcccgctgct tggcgccgcg
     6421 gcggatctgc tgcgcaacgc cgccagtcag gaagacacga ttgttgccct tggcggcacg
     6481 gcgctcgagc aggagctgcg gcagcacaga atgcttgccg aacg
//
LOCUS       DATGEM010000151         6896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1014190, whole genome shotgun sequence.
ACCESSION   DATGEM010000151 DATGEM010000000
VERSION     DATGEM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014190"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VKQ27_04720"
     CDS             complement(<1..848)
                     /locus_tag="VKQ27_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88267.1"
                     /translation="MAAEFDLVIRGGSVVDGTGRAPIEADIGIKGNRIAAIGTLAKNG
                     AEEIDAKGKLVTPGFVDIHTHYDGQAVWDGHMTPSSMHGVTTAVMGNCGVGFAPCKPA
                     DREKLVELMEGVEDIPGSVMHEGLKWEWESFSEYLDALDRRNRDIDLCALLPHAAVRV
                     FVMGERAITLENANQADIAQMREIAADAMRAGAFGFSTSRSLSHKSLKGDPTPTLRAQ
                     EDELTGIALGMRDAGSGFLEVVSEWAPDPKAEFDMLRRVVERCGRPCVFTLTQRHTRP
                     TVWRDLL"
     gene            complement(907..1770)
                     /locus_tag="VKQ27_04725"
     CDS             complement(907..1770)
                     /locus_tag="VKQ27_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLY88268.1"
                     /translation="MRRHVLASLLVWLICAGTSAAQDAVQRGEYLVHVGGCISCHTVP
                     GGTPFAGGRALATPFGTFYSPNITPDADTGIGRWTDTQFLRALREGKRPDGAYYFPVF
                     PYTSFTNITDADALAIKAYLFSLKPVHQVDRAHDVAFPFSWRFLQFGWRKLFFHEGPF
                     RPDPAHDVAYNRGAYLMTALAHCGECHTPRNSLGGLRSSLALSGTRDGPDGQAVPNIT
                     PDRATGIGDWETDDIVTLLQTGRTPEESSVTGAMREAVRDGLHFLTDQDAHAIAAYLQ
                     GQKPIAHKVGG"
     gene            complement(1773..2294)
                     /locus_tag="VKQ27_04730"
     CDS             complement(1773..2294)
                     /locus_tag="VKQ27_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLY88269.1"
                     /translation="MPLTASAGINRGRNLSAMEREGGIGVRIKILACAMILGMAAAAS
                     AEDGQDAFHHRQDVMKQMGRALYRTVGGVAKGKAEFGPDTVAAAETIARLAPDIPTLF
                     PPGSDVPDSNMKPAIFAVPDKVKQLGAGVQADMPAFVEDVKSGDKTRIASAYATMAKA
                     CDACHSEFRRQEK"
     gene            2334..3662
                     /locus_tag="VKQ27_04735"
     CDS             2334..3662
                     /locus_tag="VKQ27_04735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY88270.1"
                     /translation="MAPRVDPVTSDSELPARTGVVVVGGGIIGTSTAFFLARKGIPVV
                     LCEKGHIAGEQSSRNWGWARKMGRDPREIPLAIEALNLWPQLNELTGAETGFRQCGIV
                     YLCKTQDDMAKREAWLEEARPYQLDTRMLTRGELATVVPGVTGDWLGGLYTASDGRGE
                     PQKAAPAIAAAAQKHGATVMQQCAVRGIETQGGRISGVVTEKGRIACDSVVLAGGVWS
                     RLFCGTLNLRLPQLKVMSSVMRTEPFNPGLETSCSGFGFGVRKRLDGGYNVASWSGNV
                     HDIVPDTFNFFSEFLPSLKLQWRNLRLRVGGKFLQDWQVPKQWALDAASPFESVRTLD
                     PEPYQPVLDQARTNLTAAFPAFREMKIAETWGGVIDVTPDGVPVISGVDSLPGFFIAT
                     GFTGHGFGIGPGAGRLMAEIVAGETPVVDPTPFRFSRFSDGSRPLPSPLV"
     gene            3759..4526
                     /locus_tag="VKQ27_04740"
     CDS             3759..4526
                     /locus_tag="VKQ27_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLY88271.1"
                     /translation="MTETVVLTRLDGVLEIRLNRPEKKNALTRDMYDAMASAFRQIDA
                     DPTLRVGLLTGTGDTFTSGNDIADFQARASSNGESSASTFLPTISNMQKPLVAAVNGA
                     AIGVGTTMLMHCDLIVAARSARFVMPFTSLGLVPEAGSSLLFPRLLGTQRASALLLLG
                     EPLDAPTAQDWGFVNRVVDDDELARVAHDIAQRLAGLPPHAVRETKRLIRHGPVELAA
                     RMEMELQLFREHLISPEAAEAFRAFMEKRKPDFSQLP"
     gene            complement(4690..5577)
                     /locus_tag="VKQ27_04745"
     CDS             complement(4690..5577)
                     /locus_tag="VKQ27_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY88272.1"
                     /translation="MSDRIPPLAALRAFATVARHGSFGRAAAALNVSTSAVSHQIRGL
                     EDALGSSLLLRARNGSGRTTVTAEGAALRQEVEQAFAQLGAACEAVRERRKRPVLMIS
                     ANGSVSSLWLAPRLAAFAAQHPSVQWKVQSIDDNEPDMVREGLDLAVLRVRRGTMLEG
                     DELLFTETVFPVCSPSLRFSGDPMELSRQTLLQEDHVSSPEKDWTTWLKLLGCGPRAK
                     ATVVGFYAFAPALAAAIAGAGVALGRSPLIDRELAAGRLVRPFGDRSLPGSWDFVIRR
                     RPGAARDVHVAQLQNFLLS"
     gene            5673..>6896
                     /locus_tag="VKQ27_04750"
     CDS             5673..>6896
                     /locus_tag="VKQ27_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLY88273.1"
                     /translation="MHFDIRLWRMTAGLRGRLVLGIVLGLLALAIGIARFAFLGQFMA
                     RLFAGAPAVTLAMPLVATLVAILVRPWLDHARTAVAHRTAARVQEALRGRLYDKIVAL
                     GPAWFGAERTGGVMLSVIDGVEQLQAWFGQYLPQVAIAACAPIAIFLFMAFWDVPVAS
                     IMLAAALFSLTAPSLVHKRTGHASRERMRAFKVFGEELLDAIQGLPTLKAFGQSGSYG
                     RMLAEKARDLSESTMMVLGTSVLTRGVTDLGCALGAAAALALGAYRVRHGEMSIEALL
                     IVLMAGTEIFRPLRDLRTVLHQGLTGQAAATAINALLDEPITAPTALSRDAGGGGAAS
                     TSSIAFEGVGFAYPGGRRAAHQGLSFSIAAGARVGVVGPSGSGKSTIVRLLLRLHDPQ
                     TGTIRIGNHDLRTVDP"
BASE COUNT         1217 a         2198 c         2228 g         1253 t
ORIGIN      
        1 agcagatcgc gccacacagt aggccgcgta tggcgctgcg tgagagtgaa cacgcatggg
       61 cggccgcacc tctccacgac gcggcgcagc atgtcgaact cggccttggg atccggggcc
      121 cattccgaca cgacctccag gaagccagac cccgcatcac gcatgccaag tgcaattccg
      181 gtaagctcat cctcctgcgc ccgtagtgtg ggtgtggggt cgcccttcag gctcttgtgg
      241 ctcaggctgc gcgaggtgga gaaaccgaac gcgccggcgc gcatcgcatc ggcggcgatc
      301 tcacgcatct gcgcgatgtc ggcctggttg gcgttctcca gcgtgatggc gcgctcgccc
      361 atcacgaata cccgaactgc tgcgtgcggt agcagtgcgc acaggtcgat gtcgcgattg
      421 cggcggtcga gcgcgtccag gtactcgctg aacgactccc attcccactt caggccctcg
      481 tgcatgacag acccgggaat gtcctccaca ccctccatca actccaccag cttctcgcgg
      541 tcggcgggct tgcagggcgc gaacccgaca ccgcaattgc ccatcaccgc cgtagtgaca
      601 ccatgcatcg aggatggcgt catatgcccg tcccacaccg cctggccatc atagtgcgtg
      661 tggatgtcga cgaagcctgg tgtgacgagc ttgcccttgg cgtcgatctc ttcggcacca
      721 ttcttcgcca gcgtaccaat ggcggcgatg cggttgccct tgatgccgat gtcagcctcg
      781 atgggcgcgc ggcccgtgcc gtcgacgacg gagccaccac ggatcaccag atcgaattct
      841 gccgccatcg gacttctccc tttcccgcgg gagtgtgcgc caatccgatc ggaccgccaa
      901 gagaggtcag ccgccgacct tgtgcgctat cggcttctgt ccctgtaggt acgcagctat
      961 cgcatgcgca tcctggtccg tgaggaaatg caatccatcc cgtactgcct cacgcatggc
     1021 gcccgttacg ctgctctcct cgggtgtgcg gccggtctgg agcagcgtca cgatatcatc
     1081 ggtttcccaa tcgccgatgc ccgtcgcgcg atccggcgtg atattcggca ctgcctgtcc
     1141 gtccggtcca tcgcgtgtcc cagacaacgc caggctggat cgcaatccgc ccagcgaatt
     1201 gcgcggcgta tgacactcac cgcaatgcgc cagcgccgtc atcaaatagg cgccgcggtt
     1261 ataggccaca tcatgcgccg ggtcgggacg gaacggccct tcgtgaaaga acagcttacg
     1321 ccagccaaac tgcaggaacc gccaggagaa cggaaacgcc acatcatgcg ctcggtccac
     1381 ctgatgcacc ggcttcagcg agaagagata ggccttgatc gccagcgcgt cagcgtcggt
     1441 gatatttgtg aagctggtgt aggggaacac gggaaaataa taagcgccgt ccggccgctt
     1501 gccctcccgc aatgcccgca ggaactgcgt gtctgtccag cgcccgatac cggtatcggc
     1561 atctggtgtg atgtttgggc tgtagaaagt gccgaagggc gtggcgagtg cacgccctcc
     1621 ggcgaatgga gttccacccg gcacggtgtg gcacgagatg caaccgccga catgcaccag
     1681 atactcgcca cgctgcacgg catcctgtgc ggcggacgtg ccggcgcaga tcagccacac
     1741 cagcagtgat gccagaacgt gccggcgcac gttcatttct cctgtcggcg gaattcgctg
     1801 tggcaggcat cgcaggcttt tgccatcgtg gcatatgctg aagctatccg tgtcttatcg
     1861 ccactcttca cgtcctcaac gaacgcaggc atgtccgcct gcacgcccgc gccaagctgc
     1921 ttcaccttgt ccggcaccgc gaatatggcc ggcttcatat tgctatccgg cacgtccgat
     1981 ccaggtggaa aaagcgtggg aatgtctggt gccagacgtg cgatcgtttc cgcggcggcg
     2041 accgtgtcag gaccaaactc tgccttgcct ttggcgacac caccgacggt tctgtagagc
     2101 gcgcggccca tctgcttcat cacgtcctgc cggtgatgga acgcgtcctg gccatcctcg
     2161 gcagacgccg ccgcagccat ccccaaaatc atcgcacaag ccagaatttt gattcgcacg
     2221 ccgatacccc cttctcgttc catggcagac agatttctac cgcgatttat tccggcgctt
     2281 gccgtgagcg gcatcgcgcg gtgtgatgcc ggtttgccaa aggagcgttc gatatggcgc
     2341 cacgcgtcga tcccgttacc tctgattccg aactgcccgc gcgcacaggc gtcgtcgtgg
     2401 tcggcggcgg cattatcggt accagtacgg cgttcttcct ggcgcgcaag ggcatcccgg
     2461 tcgtgctgtg cgagaagggt catattgccg gcgagcagtc gagccgcaac tggggctggg
     2521 cacgcaagat ggggcgcgat ccgcgggaaa ttccgctggc gatcgaggcg ctgaatctct
     2581 ggccgcagct gaatgaactg accggcgcgg agacgggctt tcgccaatgt ggcatcgtct
     2641 atttgtgcaa gacgcaggac gatatggcaa aacgcgaggc ttggctggag gaggcacgcc
     2701 catatcagct cgacacgcgc atgctgactc ggggcgaact ggcgacggta gttccaggag
     2761 tgaccggcga ctggctcggc ggcctctaca ctgcgagcga tggccgcggc gagccgcaga
     2821 aggcagcgcc ggcgatcgcc gcagccgcac agaagcacgg cgccactgtc atgcagcagt
     2881 gcgcggtacg cggcatcgag acgcaaggtg ggcgcatctc gggcgtggtg accgagaaag
     2941 gccggatcgc ctgcgacagc gtcgtgctgg ccgggggggt gtggtcgcgg ttgttctgcg
     3001 gcacgctcaa tctgcgactg ccgcagctca aggtaatgtc gtcggtgatg cgcacagagc
     3061 cattcaatcc gggactagaa acctcttgca gcggctttgg cttcggcgtg cgcaagcggc
     3121 tcgacggcgg ctacaatgtg gcgagttgga gcggcaatgt gcatgatatc gtgcccgaca
     3181 cgttcaactt cttctcggaa ttcctgccgt cactaaagct gcaatggcgc aacctgcgat
     3241 tgcgggtcgg tgggaagttt ttgcaggact ggcaggtgcc gaagcagtgg gcgctcgacg
     3301 cggcgtcacc gtttgagagc gtgcgcacgt tggacccgga gccatatcag ccggttctgg
     3361 accaggcgcg caccaatctg acagcagcat tccctgcatt ccgtgaaatg aagatcgccg
     3421 aaacgtgggg cggtgtaatc gatgtgacgc cggatggcgt gccggtgatt tccggtgtgg
     3481 attcgttgcc agggttcttc atcgcgaccg gcttcaccgg ccacgggttt ggcatcgggc
     3541 caggcgccgg ccggctgatg gcggagatcg tcgccggcga aacgcccgtt gttgatccca
     3601 caccgttccg attcagccga ttcagcgacg gatcgcggcc actgccatca ccgttggtct
     3661 gatccgcgca gcatcatctg ccgcaccgcc actttgattg ccggtgcctg caaaatcagc
     3721 tacgcgacgc agcacagaac atgggaggcc gcggcccgat gactgaaacg gtcgtcctca
     3781 cgcgtctgga tggcgtgctg gaaatccggc tgaacaggcc agagaagaag aacgcgctca
     3841 cccgcgacat gtacgacgcg atggcctccg cgttccggca gatcgatgcg gacccgacgt
     3901 tgcgggttgg cttgctgacg ggcactggcg acacattcac ttcgggcaac gatatcgctg
     3961 attttcaggc acgtgcgtcg agcaatggcg aaagctcggc ctcgacgttc ctgccgacga
     4021 tctccaatat gcagaagccc ctggtcgccg cagtgaacgg cgcggccatc ggtgtcggca
     4081 cgacaatgct gatgcattgc gacctgattg tcgcggcacg gtcggcgcgc tttgtgatgc
     4141 catttaccag tcttggactg gtgccggaag ctggaagctc gctgctgttc cctcgcctgc
     4201 tcggcacgca gcgcgccagc gcgctgttgc tgctgggaga gccattggat gcgcccacgg
     4261 cccaggactg ggggttcgtg aaccgcgtgg tggacgatga tgagctggct cgggtcgccc
     4321 atgatatcgc gcaacggctg gcggggctgc cgccgcacgc ggtgcgcgaa accaagcggc
     4381 tgatacggca cggaccggtc gagcttgctg cgcgcatgga gatggagctg cagttgttcc
     4441 gtgagcacct catatccccg gaagcggccg aagcgttccg cgcctttatg gaaaaacgga
     4501 agcctgactt ctcgcaactc ccgtaagggt cagcctttgc cggcggacac tcacgatagt
     4561 tagtgagctt gatcgcgttc gcgggatcga accgcacata atccgacatg cgggaacttg
     4621 ggatgcggta aagacgcacc atggcctcgc gcgtcgaagt ctcgctcctg cgccccgccc
     4681 cgattgccgc taagacaaca ggaaattctg cagctgcgcc acatgcacat cgcgcgcggc
     4741 accgggacgg cggcggatca cgaagtccca ggatcctggc aacgagcggt cgccgaacgg
     4801 ccgcaccagc cggccagccg ccagttcccg gtcgatcagc ggactgcggc caagtgcaac
     4861 gcctgctccg gcaatcgccg ctgccagcgc aggagcgaac gcgtagaatc ccacgacagt
     4921 cgccttggcg cgcgggccac agcccaacag cttcaaccac gtcgtccaat ccttctccgg
     4981 actggacaca tgatcctcct gcaacagggt ctgccgggag agctccatgg gatcgccgct
     5041 gaagcgcaac gaagggctac agacaggaaa cacagtctcg gtgaacagca actcgtcgcc
     5101 ttcgagcatc gtaccccgac gcacccgcag caccgcgaga tccaggccct cgcgcaccat
     5161 gtcaggctcg ttatcgtcga tcgactgcac tttccattgc acggaggggt gctgcgcggc
     5221 aaaggccgcc agacgcggcg ccagccacaa tgacgacacc gatccgttcg ccgagatcat
     5281 cagcactggc cgcttgcgcc gttcgcgcac cgcctcgcac gcggcgccca gctgagcgaa
     5341 ggcctgttcc acctcctgcc gtaacgcagc cccctcggca gtgaccgtgg tgcggccgct
     5401 gccgttgcgc gcccgtagga gcagcgacga gcccagcgca tcttccaggc cacggatctg
     5461 gtgactgaca gcgctggtgc tgacgttcaa agctgccgcg gcgcggccga aacttccgtg
     5521 gcgcgcgacg gtggcgaagg cgcgcaaggc cgcgagaggg ggaatccggt cgctcatggc
     5581 cgttcaatct agctcaacag cggttgaaaa acgagcgttt gcatgatgcc gaaccgccga
     5641 acaggatggg gtcgacaagg agttcgcaac taatgcactt cgacatcagg ctttggcgca
     5701 tgacggccgg gctccgcgga cgcctggtgc tcggaatcgt gctcgggctg ctggcgctgg
     5761 ccatcggcat agcgcgcttc gcgttcctgg ggcagttcat ggccagactg tttgccggtg
     5821 cgcctgccgt cacgctggcc atgccgctcg ttgcgacact ggtggcgatc ctcgtgcgcc
     5881 cctggctgga tcacgcgcgc actgccgtgg cgcaccgcac agcggcgcgc gtgcaggaag
     5941 cactgcgcgg tcggctatac gacaagattg tcgcgctggg gccggcctgg ttcggcgcgg
     6001 agcggacggg cggcgtgatg ctctcggtga tcgatggggt ggagcagttg caggcgtggt
     6061 tcggtcagta cctgccgcaa gtcgcgatcg ccgcgtgtgc cccgattgcg atcttcctat
     6121 tcatggcgtt ctgggatgtg ccggtcgcgt cgatcatgct ggccgcagcg ctgttcagcc
     6181 tgacagcgcc gtcgctggtg cacaagcgca cgggacatgc gtcgcgcgag cggatgcgcg
     6241 ccttcaaggt gtttggcgag gaactgctgg atgcgatcca ggggctgccg acgctgaagg
     6301 cgttcggcca gagcgggtct tatggccgca tgctggcgga gaaggcgcgc gatctatccg
     6361 agagcacgat gatggtgctc ggcaccagcg tgctgacccg cggcgtcacg gacctgggct
     6421 gcgcgcttgg cgcggcggcg gccctggcgc tcggggcgta ccgggtgcgg cacggcgaga
     6481 tgagcatcga ggcgttgctg atcgtattga tggcagggac ggagattttt cggccactgc
     6541 gggatctgcg gaccgtgctg catcaggggc tgaccggaca ggcggctgcg actgcgatca
     6601 atgcactgct ggatgagcca atcactgcac ccaccgccct ctctcgcgat gcggggggag
     6661 ggggcgccgc ctccacgtcc agcattgctt ttgaaggcgt tgggttcgct tatccgggcg
     6721 ggcgtcgcgc tgcgcaccag ggactttcat tttccatcgc ggccggcgcg cgcgttggcg
     6781 tggtcggtcc cagcggttcg ggcaagtcca ccatcgttcg gctgctgctg cggctgcatg
     6841 atccacagac cggcacgatc cgcatcggca accatgatct gcgcacggtc gatccc
//
LOCUS       DATGEM010000152         4018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1013918, whole genome shotgun sequence.
ACCESSION   DATGEM010000152 DATGEM010000000
VERSION     DATGEM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013918"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VKQ27_04755"
     CDS             <1..234
                     /locus_tag="VKQ27_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY88274.1"
                     /translation="EPVSLALVDYAMPMMSGYEFAGLARDIQPDLPIVYVTGAVDALC
                     ADQQALGDPVVVKPYSRAALLRTVREMALPLKR"
     gene            complement(223..1722)
                     /gene="xseA"
                     /locus_tag="VKQ27_04760"
     CDS             complement(223..1722)
                     /gene="xseA"
                     /locus_tag="VKQ27_04760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLY88275.1"
                     /translation="MNDLATPPTSNVPEYTVSEISGAVKRTLEGNFGRVRVRGEITEL
                     KRYPSGHFYFSLKDEGAKIAGVVWKSSVSRLGMVPENGVEVIATGRISSYAERSSYQL
                     ITERMEYAGAGALLARIEMLRVRLADEGLFDAARKKPLPVLPSVIGVVSSERGAVIQD
                     IRTTIARRFPRTVLLWPVPVQGEGAAQRIAAAIEGFCRVTPQPDVLIIARGGGSLEDL
                     MAFNDEAVVRAVAACRIPLISAVGHETDTTLIDYVSDRRAPTPTAAAEMAVPARADLV
                     ADLEHKAARLIGGLNRLAQERRLRLSRAERGLPDAPTLLGGARQRLDDRAERLVLALP
                     NLLHKRQATLDGAVRRLPDLPAMLRAARERVQERGLRLIIALPNVVERRRGTLNLCGQ
                     KLVAGLRHAVSGIYGGASRTLGRLSTAPLLATLRETKVRLEGTGARLEAVSPLAVLQR
                     GYAVVSDAAGHPLTSAAAIKPGLRLRLRFADGEAGATADRPERQGKLPL"
     gene            1784..3226
                     /locus_tag="VKQ27_04765"
     CDS             1784..3226
                     /locus_tag="VKQ27_04765"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HLY88276.1"
                     /translation="MQCKTGAEHIESLKDRRTVYLDGALVPDVTTHPAFRNAVASAAS
                     LYDYQAANHDLMTFLPVGATRRVNRGWQMPRSYDDLAQRRKALEAWAQQSYGFMGRSP
                     DHVASALIGQRMGIEVFTRHSPARAKALADYVDYATRNDLFLTYVIVNPQADRSKDWG
                     NQPEELVAQIVDEDTTGLTIRGAKMLGTSSIMANEVLVANLQPLKPGEEPLAFCCALP
                     MNTSGLRVLSRKSHEAHAVSVYDNPLSSRFDENDALLYFDDVKVPWDRVFVHRDTDMS
                     RAQFHDTPGHVFQNYQAQVRLSVKLKFLVGLAHKITEAIGTTSMPQVREQLGLLAANA
                     GMVEAMVAGMEAAGGMRGEFYVPNRHMLYAAHVTTQDLYPRMIHLIRDLAGGSLIMLP
                     SSFRDWDNPVLAPIIRKSQRSPNFTPEEKVKLMKAAWDALGSEFASRHVQYEMFYAGA
                     RFVTAGHSYRTFDWNAARGMVDRLLSSYDL"
     gene            3270..>4018
                     /gene="purD"
                     /locus_tag="VKQ27_04770"
     CDS             3270..>4018
                     /gene="purD"
                     /locus_tag="VKQ27_04770"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HLY88277.1"
                     /translation="MRVLVIGSGGREHALCWSLVASPVLTKLYCAPGNPGIAQVAECV
                     PIGVMDFAALVAFAQDNEIDLVVPGPEAPLVAGITDAMEAASIPCVGPSRAAAQLEGS
                     KAFTKELCDAAGIPTAQWERFDDIEAAREFVRRRGVPIVVKADGLAAGKGVVVATTEA
                     EALAAIDAMMEARTFGEAGSSVVLEECLAGEEVSLFALCDGTTALPLGAAQDHKRVGE
                     GESGPNTGGMGAYSPTPSFPPAAQQAAMDGI"
BASE COUNT          723 a         1364 c         1263 g          668 t
ORIGIN      
        1 gagcccgtga gcctcgcgct ggtcgactat gcgatgccga tgatgtcggg atatgaattc
       61 gccggcttgg cccgggatat ccagcccgac ctgccgatcg tatatgtgac aggcgcggtg
      121 gacgcgctct gcgccgacca gcaggcgctc ggcgatcctg tcgtcgtcaa accctactcg
      181 cgtgccgctc tgctccgaac ggtgcgtgaa atggcgttgc ctctaaagcg gtaacttccc
      241 ttggcgctcc ggccgatcag cagtggcgcc agcttcgcca tcagcgaagc gcagccgtaa
      301 ccgcaagcct ggtttgatgg cggccgcact ggtcagcgga tggcccgcgg catccgacac
      361 gacggcgtag ccgcgttgca gcacggccag tggcgatact gcctctagtc tcgcgccggt
      421 gccctccagg cgcaccttgg tttcgcgcag cgtggcgagg agcggggcgg tcgacagacg
      481 tccgagcgta cggcttgcgc caccatagat acccgacacg gcgtgacgca gccctgcgac
      541 cagcttctgg ccacacaggt tgagcgtccc tcgcctccgc tccacaacgt tgggcaatgc
      601 gatgatcagg cgcagtccac gttcctgcac gcgctcgcgc gccgccctca gcatggcagg
      661 cagatccggc agacgccgga ccgcgccatc cagagtggcc tgacgcttgt gcagtaagtt
      721 gggcagcgcc agcaccagcc gttccgcccg atcatccagc cgctgccgcg cgccgccgag
      781 cagcgtcggc gcatccggca gaccgcgctc cgcgcgcgac agccgcaggc gccgctcctg
      841 cgccaggcgg ttcagcccgc cgatgagccg cgcagcctta tgttccaggt cggccaccag
      901 atcggcccgg gcaggcaccg ccatctccgc cgccgcggtc ggcgtgggcg cacggcgatc
      961 agagacgtag tcgatcagcg tcgtgtccgt ctcgtgaccc actgccgaga tcagcggaat
     1021 gcggcaggcc gcgacggcac ggaccacggc ctcatcattg aacgccatca ggtcttccag
     1081 gctgccgccg ccccgcgcga tgatcagcac atctggctgc ggcgtcaccc gacagaaccc
     1141 ctcgatcgcg gcggcaatgc gttgggccgc gccctcgccc tgtaccggca ccggccacag
     1201 cagcaccgtg cgcgggaagc gccgggcgat cgtcgttcgg atgtcctgga tcaccgcgcc
     1261 ccgctcggag ctcaccaccc cgatcaccga cggcagcacc ggcagcggct tcttgcgtgc
     1321 ggcgtcgaac agcccctcgt cggcgagccg tacacgcagc atctcgatcc gcgccagaag
     1381 cgcaccggcg cccgcatatt ccatccgctc ggtgatgagc tgatacgagg agcgctcggc
     1441 ataagacgag atgcgcccgg tggcgatcac ctcgacgccg ttctcgggca ccataccgag
     1501 acggctgacc gaggacttcc agaccacgcc ggcaattttc gcgccttcgt ccttcaggga
     1561 gaaatagaag tggcccgacg ggtagcgctt cagctcagtg atctcgcccc gcacacgcac
     1621 ccgcccgaag ttgccctcca gcgtgcgctt caccgccccg gagatctccg agacagtgta
     1681 ttcggggaca ttggaggtgg gcggagtcgc caggtcgttc atgtggtgca gagtaggcga
     1741 tcgcacggcc gacagcgagc gaccgaacca gggagatccg cagatgcaat gcaagaccgg
     1801 cgcggagcac atcgagtcac tgaaggatcg ccgaaccgtc tatctcgacg gcgcgctggt
     1861 ccccgacgtg acgacacatc cggcgttccg caatgccgtc gcttccgctg cctcactcta
     1921 cgattatcag gccgccaacc atgacctgat gaccttcctg ccggttggcg cgacgcgtcg
     1981 ggtcaatcgc ggctggcaga tgccgcgcag ctacgacgac cttgcgcagc ggcgcaaagc
     2041 gctcgaagcc tgggcacagc aaagctacgg cttcatgggg cgctcgccgg accacgttgc
     2101 ctctgcgctc attggccagc gtatgggcat cgaggtcttc accagacaca gccccgcgcg
     2161 cgccaaggcg ctggccgact acgtggacta cgccacccga aacgacctgt tcctgaccta
     2221 cgtgatcgtc aatccgcagg ccgatcgttc gaaggactgg ggcaatcagc cggaagaact
     2281 ggtggcgcag attgtcgatg aagacacaac cggcctgacc atacgtggcg ccaagatgct
     2341 gggcaccagc tcgatcatgg ccaatgaggt gctcgtggcc aatctgcagc ccctgaagcc
     2401 aggcgaagaa cccctcgcct tctgctgcgc cctgccgatg aacacgtccg gcttgcgcgt
     2461 actgtcgcgc aagagccacg aagcccacgc agtgtctgtc tacgacaacc cgctgtcgtc
     2521 gcgtttcgat gagaacgatg cgctgctcta cttcgatgac gtgaaggtgc cgtgggatcg
     2581 cgtgttcgtc catcgcgata ccgacatgag ccgcgcgcaa ttccacgaca caccgggcca
     2641 cgtgttccag aactaccagg cccaggtgcg cctgtcggtg aagctgaaat tcctcgtcgg
     2701 cctcgcgcac aagatcaccg aggccattgg cacgacctct atgccgcaag tgcgcgaaca
     2761 gcttggcctg ctcgccgcca acgccggcat ggtggaggcg atggtcgccg gcatggaagc
     2821 cgcgggcggg atgcgcggcg agttctatgt gccgaaccgc cacatgctgt atgcagcgca
     2881 cgtcacgaca caagacctgt atccacgcat gatccacctg atccgcgacc tggccggcgg
     2941 atcgctgatc atgctgccat cgtcgttccg cgattgggac aacccggtgc ttgcgccgat
     3001 tatacgcaaa tcgcagcgct cgccgaactt cacgcccgag gagaaggtca aactgatgaa
     3061 agctgcgtgg gatgcactcg gctcggaatt cgcctcgcgc cacgttcaat acgagatgtt
     3121 ttacgccggc gcccgcttcg tcaccgcggg ccacagctat cgcacgtttg actggaatgc
     3181 cgcgcgcggg atggtggatc gtctgctgtc cagctacgat ctctgaacgc actccgtcag
     3241 ctcgagtgca ggcgggcaag gagagcggta tgcgcgttct ggtgatcgga tccggcggcc
     3301 gtgagcacgc actgtgctgg tcgctcgtcg ccagtcctgt gttgaccaaa ctctactgcg
     3361 cgccaggcaa ccctggtatt gcccaggtcg cagaatgtgt gccgattggc gtaatggact
     3421 tcgccgccct cgtcgctttt gcgcaagaca acgagatcga tcttgttgtg ccgggccccg
     3481 aagcgccctt ggtcgcaggc atcaccgatg cgatggaggc agcatccatt ccctgcgtcg
     3541 gtccatcacg cgcggccgcg cagcttgaag gcagcaaggc cttcaccaag gaactgtgcg
     3601 atgcggccgg cattcccact gcgcaatggg aacgcttcga cgacatcgaa gccgcacgcg
     3661 aattcgtgcg tcggcgcggt gtgccaatcg tggtgaaggc ggatggattg gccgccggca
     3721 aaggcgtcgt ggtggccacg accgaagccg aggcactcgc cgccatcgat gccatgatgg
     3781 aagcgcgcac cttcggtgaa gccggctcat cggtggtgct ggaagaatgc ctcgccggcg
     3841 aggaagtttc gctcttcgca ctgtgcgacg gcaccaccgc cctgccgctt ggcgccgcgc
     3901 aggatcacaa gcgcgtcggc gaaggcgaga gtgggccgaa caccggaggg atgggtgcct
     3961 actcgccaac gccgtcattt cctccggcgg cacaacaggc agcgatggac ggcatcat
//
LOCUS       DATGEM010000153         8139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1555905, whole genome shotgun sequence.
ACCESSION   DATGEM010000153 DATGEM010000000
VERSION     DATGEM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555905"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VKQ27_04775"
     CDS             <1..680
                     /locus_tag="VKQ27_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HLY88278.1"
                     /translation="DIADSSELAADEKIARLDTMEDVLLGRRDMGSPSALKLRASLAE
                     TGVTARHSLDLLVAFRRDATKLRYADWGELYDYCRYSAMPVGRHVLDLHGEDRATYTP
                     SDALCTSLQVLNHLQDCAKDLAALDRCYMPAVDVEELRAPAASARLRRVFDMLLDHVD
                     TLNAAAIDLPRLTRSRRLRLETAVIVGLAHRLTRRLRRGDPVARRVRLTKGDAVGSLL
                     AALRWLP"
     gene            677..1528
                     /gene="hpnD"
                     /locus_tag="VKQ27_04780"
     CDS             677..1528
                     /gene="hpnD"
                     /locus_tag="VKQ27_04780"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HLY88279.1"
                     /translation="MTPLGVAQADLDEVEALVRSAGTSFYRGMRVLPPDRRHAMYAIY
                     AFCRMVDDVADEPGEFSTKLPQLAAWRERVADMYRGNSDGPVTRVLVAAVQRFNLRTD
                     DFLAVIDGMQMDCETIIVAPDLVTLDLYCDRVAAAVGRLSVRVFGDSSPPADRVAYSL
                     GRALQLTNILRDIQEDAERGRLYLPREWLDEAGIPHDPMAALRSPNLPLVCKRVARLA
                     HEHFRAATEAMRQCDAKAMKPARMMGATYAAILSRLESSGWSKPHERVSLPTWQKLWL
                     ALRYGLA"
     gene            1547..3235
                     /locus_tag="VKQ27_04785"
     CDS             1547..3235
                     /locus_tag="VKQ27_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLY88280.1"
                     /translation="MRRLLLLVVAVCLTAIAAHAQQAEHRVALVIGNAGYQHVERLGN
                     PGNDARLIADTLKKSGFTLVGGGAQLDLDKAGFDRLVQEFGKQIQGADVALFYYAGHG
                     MQVDNTNWLVPVDANPTRQQDLDFQMVNAELVLKQMDGANTRLNILILDACRNNPFAN
                     LGTRGVAGGLAQMKAPDGTMISFATQPGNVAVDGTGADGPYAAALAEAIRKPGLDIFH
                     LFNQVGLSVKQATQGNQQPWVSSSPIDGEFYFSRTEAAADDAAIPAIEPVAAKPSATQ
                     MPPEPTVQATRDLAEKGQAAAQIDLGINYAKGIGVQKDYATALQWFQKAADQGDAHAQ
                     FYVGLMHERGYSVPHSYATALIWYRKSAEQNFPAAQIAMARFNARGLGVPRDRAQQVM
                     WLRRAAEQDNALAEFILGNLYRLGIGVPKEPATAAQWYQRAVAKDYGPAEARLGLMYM
                     HGFGVQKDYAQSMQLLHRAADKTNALAQNGLGELYVHGAGVPRDYAQALHWFRLAADQ
                     GNTFAAFNLGMLYADGHGVPRDPREATKWLQQSAGAGNEMAIAQLGKMGVPVAR"
     gene            3323..4219
                     /locus_tag="VKQ27_04790"
     CDS             3323..4219
                     /locus_tag="VKQ27_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HLY88281.1"
                     /translation="MANEKMAAQMSSKGGFIAALDQSGGSTPGALRLYGIPDSAYSGD
                     AEMFKLMHEMRTRIVTAPAFTGAKVIGAILFEGTMDGKVGDKPVPSFLWDDRGVVPFV
                     KVDKGLEAEADGVRMMKPMPGLDTLASRAAGAGVFGTKMRSVIEHASRTGVAAIVAQQ
                     FEVGAQIAKHGLMPILEPEVNVKSPDKADAEGLLLAEIQRHADKLPSDRQVMLKLTIP
                     TVPDLYAPLIKHPRIMRVVALSGGYTRDDACKRLATNHGMIASFSRALVEGLTHSMTD
                     TAFNAALAKSIDEIYQASTVKA"
     gene            4632..4940
                     /locus_tag="VKQ27_04795"
     CDS             4632..4940
                     /locus_tag="VKQ27_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88282.1"
                     /translation="MARAILMYIPVIALAGGWIAIGAVQGESHELPLQSIVNGHRLQP
                     NQEELNAIRRPDLSAPESNEIDKLYGVLLHCAASSCSAEDHATLSRPQCDAASASSSC
                     "
     gene            complement(4955..5029)
                     /locus_tag="VKQ27_04800"
     tRNA            complement(4955..5029)
                     /locus_tag="VKQ27_04800"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4995..4997),aa:Gln,seq:ttg)
     gene            complement(5077..5916)
                     /locus_tag="VKQ27_04805"
     CDS             complement(5077..5916)
                     /locus_tag="VKQ27_04805"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLY88283.1"
                     /translation="MPDTLIEPAPAKANLFLHVTGRRADGYHLLDSLVVFAAAGDVLR
                     GEVAGGLTLEVQGPFAASLSGEPDNLVLRAARSLGGRGARLVLEKNLPVASGIGGGSA
                     DAAAALRLLCRLWKLAPPDLPGIALALGADVPVCLARRPSRMGGIGERLDAAPRLPRC
                     GLVLVNPGVAVATADVFRARRGDWSAPVELPRAWPDVTAMASDLRELRNDLEPPAIAL
                     QPVIGEVISALAAVPGCRLARMSGSGATCFGLFDDPLVAAEVAMRLRRADWWTWGGPL
                     ADS"
     gene            complement(5923..7620)
                     /locus_tag="VKQ27_04810"
     CDS             complement(5923..7620)
                     /locus_tag="VKQ27_04810"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88284.1"
                     /translation="MQHVQSARRAFLLAISLLSACGAADISPSKGASHGAIGAPSGVF
                     GDYLVGRFAMSQADPQTAATQFLRGLAVRPGDADLVQSAFIACLVSGRTEAVQLAREL
                     PDSQAAQLLLGEVAARAGHWQAAEQQFRTLPHQGLTQFLQPMLVAWAQQGDGRTDAAL
                     ATLRPYLDGQRFRGVYALHAALIADQAGRTAEAAKLYHTAQVEFGGTNLRLAQMLASW
                     EMRHGHPAEAQHVLASMATSDTPQMAIALPALLAATKTPPVRNATDGIAEAYLALAAT
                     LRQQDAGNFAMVLLRLAMDLRPDLTAARILAADILQDDQHYDSALQMLAPVGADDPLS
                     PVIRLQRAAIAEQLGHTDEAMQELQRIAHDYPDSPLPMVRQGDLLRTKQRFTDAIAAY
                     DRAVALIKTPTESDWLLFYDRGTSYDRAHQWDKAQADFQHALKLSPEQPYVLNYFGYT
                     WADKSEHLPQARDMVEKALQHRPNDGAIVDSLGWVMFRQGQITDAVKTLERAVELQPE
                     DAEINGHLGDAYWAAGRKLEAAYQWRRALTFNPEPDDAAKLEAKLQNGGQATLVSGQ"
     gene            complement(7708..>8139)
                     /locus_tag="VKQ27_04815"
     CDS             complement(7708..>8139)
                     /locus_tag="VKQ27_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLY88285.1"
                     /translation="TYLLRGKAPWTFHHAHPDNDTLIDAQSAPRIEYPRPDGVLTFDR
                     LSSVFISNTNHEENQPSHLRLADPGVAISLNWQRFRSPEARYCPAGVYEIVGAEAGDP
                     RLQINAQNCVHCKTCDIKDPAQNIDWVTPEGGGGPNYPGGM"
BASE COUNT         1482 a         2616 c         2667 g         1374 t
ORIGIN      
        1 acgacatcgc tgattcgtcg gagcttgcgg cggacgagaa gatcgcgcgg ctcgatacga
       61 tggaggacgt gctgctggga aggcgcgaca tgggttcgcc gagcgctctg aagctgcgtg
      121 cgagcctggc agaaaccggc gtcaccgcac gtcattcgct ggatctgctg gtcgcgttcc
      181 ggcgcgatgc gaccaagctg cgatacgcag actggggcga gctctacgac tactgccggt
      241 actcggcgat gcccgtagga cgtcacgtgc tggatctgca tggcgaggat cgcgcgacct
      301 acacaccgtc cgatgccctg tgcacatcgc tgcaggtgct gaaccatctg caggactgcg
      361 caaaggatct ggcggcactc gatcgctgct atatgcccgc tgtcgatgtc gaggaactgc
      421 gcgctccggc cgcgtctgct cggctccggc gggtgttcga tatgctgctc gatcatgtcg
      481 atacgctgaa cgccgcggcg attgacctgc cgcgtttgac acgaagccgg cgattgcggc
      541 tggaaacagc ggtgattgtg ggactggcac atcggctgac tcgcaggctg cggcgcggcg
      601 atccggtggc ccgccgggtg cggctgacca agggtgatgc tgtcggcagc ttgcttgcgg
      661 cgttgcggtg gttgccgtga caccgcttgg cgtggcgcaa gctgatctcg atgaggtcga
      721 ggcgctcgtc cggtcagccg gaacgtcctt ctatcgtggc atgcgcgtgc tgccgcccga
      781 tcggcggcac gcgatgtacg cgatctacgc cttctgccgc atggtcgatg atgtggccga
      841 cgagcctggc gagttctcga ccaagctgcc ccagctcgcc gcctggcgcg agcgggtcgc
      901 cgatatgtat cgagggaact cggacgggcc ggtgacgcgc gttctggtcg ccgccgtgca
      961 gcgcttcaat ctgcggacag acgatttcct tgccgtgatc gatggcatgc agatggactg
     1021 cgagaccatc atcgtcgcac ccgacctcgt cacgctcgac ctctattgcg atcgtgtcgc
     1081 cgccgccgtc gggcggctgt cggtgcgcgt gtttggtgac tcatcgccgc ccgccgatcg
     1141 ggtcgcctat tcacttggcc gggcgctgca gctcaccaac atcctgcgcg atatccagga
     1201 agatgcggag cgcgggcggc tctacctgcc gcgcgagtgg ctcgacgagg ccggcattcc
     1261 tcatgacccg atggccgccc tcaggtcccc caacctcccg ctcgtctgta agcgagtggc
     1321 caggctggca cacgaacatt tccgtgccgc gacggaagcg atgcgccaat gcgatgccaa
     1381 ggcgatgaag cccgcgcgca tgatgggggc gacctacgcc gcgatcctct cgcgactgga
     1441 aagcagcggc tggtccaagc cgcacgaacg tgtgagtctg cccacatggc aaaagctgtg
     1501 gctcgcgctg cgctacggcc tcgcctgaaa caacagggaa cgtcagatgc gccgacttct
     1561 gctgctggtc gtggccgttt gcttgaccgc catcgctgcg catgcgcaac aggctgagca
     1621 ccgcgttgcg ctggtcatcg gcaacgccgg ctaccagcat gtcgaacggc tgggcaatcc
     1681 cggcaacgac gccaggctga ttgctgacac actgaagaaa tctggcttca ccctggtcgg
     1741 tggcggcgca cagctcgacc tcgacaaggc cggtttcgat cgtctggtgc aggagttcgg
     1801 caagcagatc cagggcgccg acgtcgcgct gttctattac gccggccacg gcatgcaggt
     1861 ggacaatacc aactggctgg tgccggtgga tgcgaacccg acgcggcagc aggacctcga
     1921 tttccagatg gtcaacgccg agctcgtact gaagcagatg gacggcgcga acacgcggct
     1981 caacatcctg atcctggatg cgtgtcgcaa caatccgttc gccaacctcg gtacgcgcgg
     2041 cgtcgcaggc ggccttgccc agatgaaggc accggacggc acgatgatct cgttcgcaac
     2101 gcagccaggc aatgtcgcgg tcgacggcac gggcgccgat ggcccttacg ccgcagcgct
     2161 tgcggaggcg atccgcaaac ctggtctgga tattttccac ctgttcaacc aggtgggtct
     2221 gtcggtgaag caggcgacgc agggtaacca gcagccctgg gtgtccagct cgccgatcga
     2281 tggcgagttc tatttctcgc ggaccgaagc agcggcggac gatgcggcca tccccgcgat
     2341 cgagccggtt gccgcgaagc cgtccgcgac tcagatgccg ccggagccga ctgtacaggc
     2401 gacgcgcgat ctggccgaga agggccaggc cgcggcgcag atcgatctcg gaatcaatta
     2461 tgccaagggc atcggcgtgc agaaggacta cgccactgcg ctgcaatggt tccagaaggc
     2521 agcggatcag ggcgatgcgc atgcacagtt ctatgtcggc ctgatgcacg agcgcggcta
     2581 ttcggtaccg cacagctacg ccaccgcgtt gatctggtat cgcaaatcgg cagagcagaa
     2641 tttcccggcc gcgcaaatcg cgatggcacg gttcaacgca cgcggccttg gtgtgccgcg
     2701 cgatcgtgcg cagcaggtga tgtggctgcg ccgcgcggcc gaacaagaca acgcactggc
     2761 cgagttcatc ctcggcaacc tgtatcgcct ggggatcggc gtgccgaagg agcccgccac
     2821 cgcggcacaa tggtatcagc gcgctgttgc gaaggattac ggaccagccg aggcgcggct
     2881 cgggctgatg tatatgcatg gcttcggcgt gcagaaggat tacgcgcagt cgatgcagtt
     2941 gttgcatcgc gccgccgaca agaccaatgc gctcgcacag aacgggctgg gtgagctgta
     3001 tgtgcatggc gctggcgtgc cacgcgacta tgcccaggcg ctgcactggt tccggctggc
     3061 agccgatcag ggcaatacgt tcgcggcgtt caatctcggg atgttgtacg ccgatgggca
     3121 cggcgtgccg cgcgatccaa gagaggccac gaaatggctg cagcaatccg cgggcgcagg
     3181 taacgaaatg gcgatcgcgc aattgggaaa gatgggcgtg ccggtggcca ggtagtgccg
     3241 atctcggcgt aatgatcagc ccgtgctaga cagccaggga caggcggcgt cagcctgggc
     3301 gcatcaatga gaggatagcg acatggccaa cgaaaaaatg gcagcgcaga tgagcagcaa
     3361 gggaggcttc atcgccgcac tcgatcagag cggcggatcg acgccaggcg cattgcggct
     3421 gtacggcatt ccggacagcg cctacagcgg cgatgcagag atgttcaagc tgatgcacga
     3481 aatgcgcacc cgcatcgtga cagcgcccgc cttcaccggc gccaaagtca tcggcgccat
     3541 cctgttcgaa ggcacgatgg acggcaaagt gggcgacaag ccggtgccaa gcttcctgtg
     3601 ggacgatcgc ggtgtcgtgc cgttcgttaa agtggacaag gggctggaag cggaagccga
     3661 cggcgtcagg atgatgaagc cgatgccggg gctggatacc ctggcgtccc gcgcggccgg
     3721 tgctggcgtg ttcggcacga agatgcgctc ggtcatcgag catgcgtcgc ggaccggtgt
     3781 ggccgccatc gtggcgcaac agttcgaggt cggcgcgcaa atcgccaagc acgggctgat
     3841 gccgatcctc gagccggaag ttaacgtgaa gagccccgac aaggcggatg cggaagggct
     3901 gctgctggca gagatccagc gccacgcaga caagctgcca agcgaccggc aggtcatgct
     3961 gaagctgacc atcccgaccg tgcccgacct gtatgcgccg ctgatcaagc atccccgcat
     4021 catgcgcgtt gtggcacttt cgggcggcta tacacgcgac gatgcctgca agcgtctcgc
     4081 gacgaaccac ggcatgatcg cgagcttctc gcgcgccctg gtcgaggggc tgacacactc
     4141 aatgaccgac accgcattca acgccgcgct ggccaagtcg atcgacgaaa tctaccaggc
     4201 atccaccgtc aaagcctgat ccgcggttcg cgggcagtaa acggccgggg tggctctgcc
     4261 gcatcggccg ttgccttttg tcgagaacac ggtctgggcc gctactcgtc ggcagtgtga
     4321 taccgcatcc tattgagcgg gaccgatcga tgctcccgtc atgccgggcg aaggcccggc
     4381 atccacgtct tcgtgcctgt gacagtcagg catcgtcatc gagaagtagt ataattccgc
     4441 agcccggacc cgtcgaaagc ctttggtatc ccgcaaccca ggaaggcccg cgccttaatg
     4501 gaacgattct ggcgcaacgt gggctgcatg gctggaaccc tgctggcacc tggtggtcct
     4561 cgtggctatg tcatcccggt tctgcactgc aggttcgtgg cagcgaccca gaacgccgtt
     4621 gggagacgaa aatggcccgc gcgatactta tgtatatacc agtcattgct ctggcaggcg
     4681 gatggatcgc catcggcgcc gtccagggcg agtcacatga gttgccgttg cagtccatcg
     4741 tcaacggtca ccgtctgcaa cccaaccaag aggagctgaa cgcgatccgc cgtcctgacc
     4801 tgtcggctcc tgaatccaac gagatcgaca agctctacgg cgttctgctg cactgtgccg
     4861 catcttcgtg ctcggccgaa gaccatgcaa ccttgtcgcg ccctcagtgc gacgcggcgt
     4921 cggcatcttc gtcctgctga cggcgcggat ctgctggctg gggcgggagg gatcgaacct
     4981 ccgaatggcg gaatcaaaat ccgctgcctt accgcttggc tacgccccat ccggcaacga
     5041 gtgcataggt cggcggccac ccgaggtaaa gcccgatcag ctgtcagcca acggtccgcc
     5101 ccacgtccac caatcagcgc gccgcaggcg catggccacc tctgccgcca cgagcgggtc
     5161 atcgaacagg ccgaagcacg ttgcgccgga gccgctcatc cgcgccaggc gacagccggg
     5221 caccgccgcc agcgcgctga tcacctcgcc gatgaccggt tgcaatgcga tggcaggcgg
     5281 ctccaggtca ttgcgcaact ctcgaaggtc gcttgccatg gcggtgacat cgggccaggc
     5341 acgcggcagc tcgacgggcg ccgaccagtc gccacggcgc gcgcggaaca cgtccgccgt
     5401 ggccacggcc acgccgggat tgaccagcac caggccgcac cgcggcagcc gcggtgcggc
     5461 gtccagccgt tccccaatac cacccatccg gctcggccgc cgagccaggc acaccggcac
     5521 gtcggcccca agcgccaggg caatgcccgg caggtcaggc ggcgcgagct tccacagccg
     5581 gcacagcaac cgcaaggcgg cggccgcatc tgccgaacca ccgccaatac cggacgccac
     5641 cggcaggttc ttctccagca ccagccgcgc gccacgaccg ccgagcgagc gcgctgcccg
     5701 caggaccagg ttgtcgggct caccggaaag gctcgcggcg aacggcccct gaacctcgag
     5761 ggtcagcccg ccagccacct ctcctcgcag cacgtcccca gccgcggcga acacgacaag
     5821 actgtcgagc aggtgatagc catcggcccg ccggccggtg acgtgcagga acagattggc
     5881 cttggccggg gccggctcga tcagcgtgtc aggcaatcgc cgctattgcc cgctgaccag
     5941 ggtggcttgg ccgccattct gcagcttcgc ttcaagcttc gccgcatcgt cgggctccgg
     6001 attgaaggtg agcgcgcggc gccactggta ggcggcctcg agcttgcggc cggccgccca
     6061 ataggcgtcg ccgagatgcc cattgatctc ggcatcttca ggctgcaatt cgacggcgcg
     6121 ttccaaggtc ttcaccgcgt ccgtgatctg cccctggcgg aacatgaccc agcccaggct
     6181 atcgacgatg gcgccatcgt tcgggcgatg ctgcagcgct ttctcgacca tgtcgcgagc
     6241 ctgcggcagg tgctccgact tgtcggccca ggtatagcca aagtagttca gcacataagg
     6301 ctgttccggt gacagtttca gcgcatgctg gaaatcggcc tgcgccttgt cccactgatg
     6361 ggcccggtcg taggaagttc cgcgatcgta gaacaacagc cagtctgact ccgtcggcgt
     6421 cttgatcagc gcgacagcac gatcgtaggc ggcgatggcg tcggtgaagc gctgcttggt
     6481 gcgcagcaga tcaccctggc gcaccatcgg cagagggctg tcgggatagt cgtgcgcgat
     6541 acgctgcaat tcctgcatcg cctcgtcggt gtgtcccaac tgctcggcga tcgccgcgcg
     6601 ctgcagcctg atcaccgggc tgagtgggtc atcggcgccg acaggtgcca gcatctgcaa
     6661 ggcggagtcg tagtgctggt catcctgcag gatatcggcg gccaggatgc gcgcggcggt
     6721 cagatcgggg cgcaggtcca tcgccaggcg caacagcacc atcgcaaagt tgccggcatc
     6781 ctgctggcgc agcgtggccg ccagagccag gtacgcctcg gcgatgccgt cagtcgcgtt
     6841 gcgcacgggc ggcgtcttgg tcgccgccag gagcgccggc aaagcaatcg ccatctgcgg
     6901 cgtgtcggac gtggccatgc tggccagaac atgctgcgcc tcggccgggt ggccgtgccg
     6961 catctcccaa ctcgcaagca tctgcgccag gcggaggttg gtgccgccga actcgacctg
     7021 cgctgtgtga tacaacttgg cagcctctgc tgtgcgccca gcctggtcag caatcaacgc
     7081 cgcgtggagg gcatagacgc cgcgaaaccg ctggccatcc aggtaagggc gcagggtcgc
     7141 cagcgccgca tcggtccggc cgtcgccctg ctgcgcccag gcgaccagca ttggctgtag
     7201 gaattgtgtc aggccctgat gcggcaacgt acggaactgc tgctcggccg cctgccaatg
     7261 accggcacgc gccgccactt caccaagcag caattgtgcg gcctgactgt ccggtagctc
     7321 gcgggccagc tgtacggcct cggtccggcc agaaaccagg caggcgataa aggcactctg
     7381 cacaagatcg gcgtcaccgg gccgaaccgc gagcccgcgc agaaattgcg tggccgcggt
     7441 ctgcggatcg gcctgcgaca tggcgaagcg gcccaccaga tagtcaccga acacgccaga
     7501 cggcgcgcca atggcgccgt ggctcgcccc cttgctgggc gaaatgtcgg ccgcaccaca
     7561 ggcagacagg agggagatcg ccaggaggaa cgcgcggcga gcgctttgga catgctgcat
     7621 ctgcacaacc tacttcatgt gcctggtcaa gtcatgagga tttgatcgaa actgcttaag
     7681 gctcgattat ccggccgatg acaaaactta catcccacca gggtaattcg gtccaccacc
     7741 gccttcgggc gtgacccagt cgatgttctg cgccgggtcc ttgatgtcgc aggttttgca
     7801 gtgcacgcaa ttctgcgcat tgatctgcag gcgtggatcg cctgcctcgg ccccgacgat
     7861 ctcatagacg ccggcagggc aatagcgggc ctcagggctg cggaagcgct gccagttcag
     7921 ggagatggct accccgggat cggcgagacg cagatgcgac ggctgattct cctcatggtt
     7981 ggtgttgctg atgaacaccg aggacaaccg gtcgaacgtc agcacaccat cgggacgagg
     8041 atactcaatg cgcggcgccg actgtgcgtc gatcagcgtg tcgttgtccg gatgggcgtg
     8101 atggaatgtc cacggtgcct tgccgcgcag caggtaagt
//
LOCUS       DATGEM010000154         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1094484, whole genome shotgun sequence.
ACCESSION   DATGEM010000154 DATGEM010000000
VERSION     DATGEM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094484"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1069
                     /locus_tag="VKQ27_04820"
     CDS             119..1069
                     /locus_tag="VKQ27_04820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLY88286.1"
                     /translation="MIRAWAAASLLALTGCAAPQQARQTVREAPPTPRPRVQLPVAPA
                     SGGASFSFAGTMSQGGMLVGTAPGGTISLVLDGADVPIDADGRFLIAFGRDHDPDATL
                     IATRRDGSTVVDKLAVAPRAWRIEALPIPKYQQPPAEFLKIRAGELAQIKAARDGAAT
                     VKSDGWQQHFLWPATARISGLFGAQRVYNGETGSYHSGIDLAVPQGTPIRAPADGVVL
                     LAASDDPFTLEGHLLMIGHGMGLDSAFLHLSHIDVKVGDIVRQGQVIGESGMTGRATG
                     PHLHWALTWRGERIDPLLVAPPMPGDQAPAAGVLSPPGGP"
     gene            complement(1047..1589)
                     /locus_tag="VKQ27_04825"
     CDS             complement(1047..1589)
                     /locus_tag="VKQ27_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88287.1"
                     /translation="MKVRHVLAASLALAAPAVAAPHPPATDARQPKQLDVVLGCRSIT
                     GATERLACFDRSVDALQSATAKHDIVVVDREVVRKTRRSLFGFNLPGGTIFDPDNRKS
                     GEPAEEEREINATIRSARMGADGYWVVVLEDGAVWHQTDGTLALAPKPGATVIIRRAT
                     LGSYFMRIGSQPGVKARREG"
     gene            1840..>4432
                     /locus_tag="VKQ27_04830"
     CDS             1840..>4432
                     /locus_tag="VKQ27_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY88288.1"
                     /translation="MTRAMRERLLATTIIAGAAVLTAPAFAQTTPPAGAAGQAPTPSV
                     APAPAAAPTGGEIVVTGSRIPTPNLTSVSPVTVVSSQDIKLQGVTRVEDLLNSLPQVA
                     AGQSSGLSNGATGTATVDLRGLGPTRTLVLINGRRLLPGDPGSSAADLNAVPSALIKR
                     VEVLTGGASSTYGADAVAGVVNFIMDTDFTGIRLDSQYNFYQHDNHSGLVQPLLQARQ
                     NAGLSGYGFPTGNSVDGGTVDATLSMGAGFDDNRGHIVGYFGYRKVKAVTQNNRDYSA
                     CTIQNKNASALQCGGSATSNPANAFLTGSPGGGTTTAYTVGQGGKLTPGLSRYNFAPT
                     NYFQRPDERYTGGFFAHYDVSDAIKPYMEFNFMDDRTKAQIAPSGDFGNTLTINCDNP
                     ALISSGNKPLVCDPGNLVNGFLGNFPLTANGGNAGIPTQFFDPTTGAPYNKGFFQVLR
                     RNTEGGPRIADLEHTEFRGVIGAKGDLGSAWSYDAYYQYGRTIYSQVYSGEFSVSRLS
                     NALDIVGDPNNPATWQCRVTAAHIDPNCQPYNLFGTPSQGAVNYVSATGFQHGIVTEQ
                     VASGSFTGQLGEYGIKSPWANDGVSINVGGEYRKETLDLQVDQEFSTGDLTGQGGATL
                     PISGNYHVIEGFGELQAPLVHENVIYDLSLNAGYRRSHYQISNGNKFDTNTFKVGLEF
                     APVKDVRFRASYNRAVRAPNLQELFATQHVALDGSTDPCAGGFDANGTTVNGNTAAQC
                     AASGVSAAQFGHIAANPAGQYNGLIGGTPSLKPEVSITKSFGAVFQPSFLPHFALTVD
                     YYDIKVKGAVQGFGADAIANTCVATGNPTACSLIHRDPGGSLWLTPGGFITDLQQNIG
                     SLHTKGID"
BASE COUNT          758 a         1442 c         1383 g          849 t
ORIGIN      
        1 atgttacctg cgcggtgacg ggcgagacga tcccgcttga ccggttgcgt tactggagcg
       61 tcgagcggca agagccctac gcctcggccg aagcctccac caaggcggaa ctcaaggctt
      121 gatccgcgct tgggcggcgg cgtcgttgct ggcgctgacg gggtgtgctg cgccgcagca
      181 ggcgcggcaa acagtccgcg aagcgccgcc cacgccgcgg ccacgcgttc agctgccggt
      241 ggcgccggcc agtggaggtg cgagcttctc cttcgcgggc acgatgagcc agggcgggat
      301 gctcgtcggc actgcgcccg gcggcacaat ctcgctggtt ctcgatgggg cggacgtacc
      361 gatcgacgcc gacggccgct tcctgatcgc cttcgggcgc gaccatgacc ccgacgcgac
      421 gctgatcgcc acccgccgcg atggctcgac cgtggtcgac aagctggcgg tggccccgcg
      481 cgcttggcgg atcgaggcgc tgcccattcc caaatatcag cagccgcccg cggaattctt
      541 gaagatccgg gcgggcgagc tcgcgcagat caaggccgcg cgcgatggtg cggcgacggt
      601 caagagcgac ggctggcagc agcattttct atggccggcg accgcgcgga tcagcgggct
      661 gttcggtgcc cagcgcgtct ataatggcga gacgggcagc tatcattcgg ggatcgatct
      721 cgccgtgccg cagggcacgc cgatccgcgc gccggccgat ggagtagtgc tgctcgccgc
      781 atcggatgat cccttcacgc tcgagggcca tctgctgatg atcggccacg gcatggggct
      841 cgacagcgcc tttctccact tgagccacat cgacgtgaag gtgggcgaca tcgtgcgcca
      901 gggtcaggtg atcggcgaga gcggcatgac cggccgcgcc accggcccgc atctccactg
      961 ggcactcacc tggcgcggcg agcggatcga cccgctgctg gtcgccccgc ccatgccggg
     1021 cgaccaggcg ccggctgccg gcgtcctcag ccctcccggc gggccttgac gccaggctga
     1081 ctgccgatcc gcatgaaata gctgcccagc gttgcacggc gaatgatcac ggtggcaccc
     1141 ggcttcggcg cgagcgccag cgtgccgtcg gtctgatgcc acaccgcgcc gtcttcgagc
     1201 acaaccaccc aatatccgtc tgcccccatc ctggcggacc gaatggttgc gttaatctcg
     1261 cgctcttcct cggcaggttc gccggatttg cggttatcgg ggtcgaagat cgtcccgcct
     1321 ggaaggttga aaccgaatag cgatcgccgc gtcttgcgga ccacttcgcg gtctacgacg
     1381 acaatgtcgt gcttggcggt ggccgactgg agcgcgtcga cgcttcgatc gaaacaggcc
     1441 agccgttccg ttgcgccggt gatcgagcga cagcctagca cgacatcgag ctgtttcggc
     1501 tgtctcgcat cggttgcagg cggatgcggg gccgccacgg ctggcgccgc cagggccaag
     1561 ctcgccgcca gaacatgtcg caccttcata tctgtctcct tgccggcatt cgcatgaact
     1621 taacgaagcg atcggcaccg gcaagagatg tgtctgttgc tgaattgcta cacatccgcg
     1681 caattgccgt cctcccgctg ccgagcggct cgttgcacgt tgctgcgttg ccccgatttc
     1741 tcctagacca tgtgtagctt ggaccggtgt gtcttttagg cgctccgccc agccagccac
     1801 gagatcctag ggatcctgac gcttcaaggg gataagacga tgacaagggc tatgcgggag
     1861 cgccttctgg cgacgaccat cattgccggc gcggcggtgc taacggcacc tgctttcgcg
     1921 cagacgactc cgccggccgg tgcggccggc caggcgccga ctccgagcgt ggcgcccgcg
     1981 cccgctgctg ctccgaccgg cggcgaaatc gtcgtcaccg gctcgcgcat tccgacgccg
     2041 aacctcacct cggtgagccc ggtgacggtc gtgtccagcc aggacatcaa gctccagggc
     2101 gtgacgcgcg ttgaagactt gctgaacagc cttccgcagg ttgccgctgg tcagtcgagt
     2161 ggtctctcca acggcgccac cggcaccgcc acggtcgatc ttcgcggcct cgggccgacg
     2221 cgcaccctcg ttctgatcaa tggtcgtcgc ctgctgccgg gtgatccggg ctcgtccgcg
     2281 gctgacctca atgccgtgcc ctccgcgctg atcaagcgcg tcgaagttct gactggtggt
     2341 gcgtcgtcga cctacggcgc ggacgccgtc gccggcgttg tcaacttcat catggacacc
     2401 gattttaccg gtatccgcct cgacagccag tataacttct atcagcacga caaccacagc
     2461 ggtctcgttc agccgttgct ccaggctcgt cagaatgccg gcctgtccgg atatggcttc
     2521 ccgaccggca acagcgttga cggtggcacg gttgatgcca cgctcagcat gggcgccggc
     2581 ttcgatgaca accgcggcca catcgtcggc tatttcggct accgcaaggt gaaggccgtc
     2641 acgcagaaca atcgcgatta cagcgcctgc accattcaga acaagaatgc gtccgcgctg
     2701 cagtgcggcg gttcggctac ctcgaatccg gcaaacgctt tcctcacggg cagccctggt
     2761 ggtggcacga ccactgcgta tacggttggt cagggtggaa agctgacgcc gggtctgtcg
     2821 cgctataact tcgcgccgac caactatttc cagcgtccag acgagcgtta caccggcggc
     2881 ttcttcgccc actatgacgt tagcgacgcg atcaagccgt acatggagtt caacttcatg
     2941 gacgaccgca cgaaggcgca gatcgcgccg tcgggtgact tcggtaacac gctgaccatc
     3001 aattgcgaca atcctgctct gatttcttcg ggcaataagc cgcttgtctg cgatccgggc
     3061 aacttggtga atgggttcct cggcaatttc ccgcttactg ccaacggcgg taatgctggg
     3121 attccaaccc aatttttcga tccgaccacc ggcgcaccat ataacaaggg cttcttccag
     3181 gtgttgcgcc gcaacacgga aggcggtcct cgtatcgcgg atctcgagca taccgaattc
     3241 cgcggtgtga tcggcgccaa gggcgatctg ggttcggcct ggtcgtatga cgcttattac
     3301 cagtatggcc gtacgattta ttcgcaggtc tattcgggcg agttctcggt ctcccgcttg
     3361 agcaatgcgc tcgatatcgt gggtgatccg aacaatccgg ccacctggca gtgccgcgtg
     3421 accgcggctc atatcgatcc gaattgccag ccctataatc tcttcggcac gccgtcgcag
     3481 ggcgcagtca actacgtgtc tgcgaccggc ttccagcacg gtatcgtcac cgagcaggtg
     3541 gcgagtggct cgttcaccgg ccagctcggc gaatatggca tcaagtcgcc gtgggcgaac
     3601 gatggcgtga gcatcaacgt gggtggcgag tatcgcaagg agaccctgga ccttcaggtc
     3661 gaccaggagt tctcgacggg cgatctcacc ggccagggtg gtgcgacgct tccaatctcg
     3721 ggcaactacc acgtcatcga aggcttcggc gaactccagg cgccgctggt tcacgagaac
     3781 gtgatctacg atctttcgct caacgcgggt tatcgtcggt ctcattatca gatcagcaac
     3841 ggcaataagt tcgacaccaa caccttcaag gtcggtctcg agttcgcgcc ggtgaaggac
     3901 gttcgcttcc gcgccagcta caaccgcgcg gtgcgtgcgc cgaaccttca ggaactgttt
     3961 gcgacccagc atgtcgcgct cgatggctcg accgatccgt gcgcgggtgg gttcgacgct
     4021 aatggtacca ctgtaaacgg caatacggct gcgcaatgcg ccgcgtctgg tgtgagcgcc
     4081 gcccagttcg gccatatcgc tgcgaacccg gctggtcagt ataatggcct gatcggcggt
     4141 acgccgtcgc tcaagccgga agtctcgatc accaagtcgt ttggtgcggt gttccaaccg
     4201 agcttcttgc cgcactttgc cctgacggtc gactattacg acatcaaggt gaagggtgcg
     4261 gtccagggct tcggcgctga cgcgatcgcc aatacctgcg ttgcgacggg taatccgact
     4321 gcttgcagcc tgatccaccg cgatcccggt ggttcgctgt ggctgacgcc gggcggtttc
     4381 atcaccgatc tgcagcagaa tatcggttcg ctccatacca agggtatcga cg
//
LOCUS       DATGEM010000155         8753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_300358, whole genome shotgun sequence.
ACCESSION   DATGEM010000155 DATGEM010000000
VERSION     DATGEM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300358"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="fabF"
                     /locus_tag="VKQ27_04835"
     CDS             complement(<1..1228)
                     /gene="fabF"
                     /locus_tag="VKQ27_04835"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLY88289.1"
                     /translation="MRRVVVTGMGIACPLGLGVEHVWRRLIAGESGIGSIQSFDTNDL
                     PCKVAGQIPAGMKADGGLDLAEWIPVKDVKKMDRFIHLGMVAATEAMEDSGWLPEAEE
                     DKCATGVMIGSGIGGLQTIYEASIQVHEGRARRLSPFFIPSALINLVSGHVSIKYGLK
                     GPNHSAVTACATGVHAIGDASRLIMWGDADVMVAGGAEAAVCALGIAGFCASRALSTA
                     FNDEPTRASRPWDKDRDGFVMGEGAGVLVLEEYEHAKKRGAKIYGEVAGYGMSGDAFH
                     ITAPAEGHDGAVRSMRAAFRNGGISPDQVQYVNAHGTSTPLGDDLELEAVEHVFGDAA
                     KGLAMSSTKSATGHLLGAAGAVEAIFSILAVRDNVAPPTLNLEEPSRESVIDRVARTA
                     QDRRIDYALSNSFGFGG"
     gene            complement(1253..1492)
                     /locus_tag="VKQ27_04840"
     CDS             complement(1253..1492)
                     /locus_tag="VKQ27_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLY88290.1"
                     /translation="MSEIADKVKKIVVEHLGVEEAKVTPEASFIDDLGADSLDTVELV
                     MAFEEAFGVEIPEDAAEKISTVKDAIEYIEKQKAA"
     gene            complement(1594..2331)
                     /gene="fabG"
                     /locus_tag="VKQ27_04845"
     CDS             complement(1594..2331)
                     /gene="fabG"
                     /locus_tag="VKQ27_04845"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HLY88291.1"
                     /translation="MFRLDGKPVLVTGSSGGIGAEIARTLHKLGAVVALSGTRQDALE
                     ALTAELGERAFVCPADLRDASAPDSLIEAAEQASGPLWAVINNAGMTRDTLALRMSDA
                     DWQMVLDVDLSAPFRLCRSALRGMLRRRAGRIINIGSIVGSTGNAGQANYAAAKAGLI
                     GMTKALAQEVAPRGITVNAVAPGFVATAMTDKLSDQQKTKLTDAIPLGRIGKPEDVAA
                     AVAYLASEEAGWVTGATLHVNGGMAMV"
     gene            complement(2334..3302)
                     /gene="fabD"
                     /locus_tag="VKQ27_04850"
     CDS             complement(2334..3302)
                     /gene="fabD"
                     /locus_tag="VKQ27_04850"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLY88292.1"
                     /translation="MPVHAFIFPGQGSQAVGMGRDLATAFPSAREVFEEVDETLRQKL
                     SRLMFEGPGEELVLTENTQPALMAHSLAVLRVLEKEGGFTLRQKAVVVAGHSLGEYSA
                     LAAAGAFDIADAARLLKLRGQTMQKAVPAGEGAMAALLGADMSQAESICAEAGPVPES
                     DPPQTEVIQPANDNGAGQIVVSGHRGAVERAVALAASKGVRRAMLLPVSAPFHCALMA
                     PAADAMAEALESTPPRAPIVPLVANVSSAKATDPAEIRELLVKQVTAMVRWRECVNAM
                     AELGCDSFIELGAGKVLSGLVRRIAPDAATASAATPAEIEALLKAL"
     gene            3561..3782
                     /locus_tag="VKQ27_04855"
     CDS             3561..3782
                     /locus_tag="VKQ27_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HLY88293.1"
                     /translation="MGQGSSNRLMRLACAMNFAAQTCRFAGRQVPLRVRYDGVQLDRG
                     YVADIIVRDEVLLELGSVEQLLRSFSRSS"
     gene            3975..4871
                     /locus_tag="VKQ27_04860"
     CDS             3975..4871
                     /locus_tag="VKQ27_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88294.1"
                     /translation="MGQFVIHTHGRLQEWVAQEKGYFAAEGLTDYILASHGLLSRDQV
                     AMQTPASLDIRHGAYQSYEQGRDASVSCACHWTVNKAASAEFGRLWGECYTVTPCGVF
                     VPANSAIKSPEDLADVAVHVGYQSGSHYTTIQALEPFLELDSIKLRFGGSPADRVDQL
                     LDGSAPAATVFGAQYYLIEQLGFRKVVDASFMIAAMVPLHVDTAEVRKYFAALRRAQG
                     DIDVSHQPYTHYYGNELPERHRALVDVRRFGPGERLVFEPYTQEMYQRTQAWIDERGI
                     FSEGPSNPHGYAESVIRLEAAE"
     gene            complement(4878..5576)
                     /locus_tag="VKQ27_04865"
     CDS             complement(4878..5576)
                     /locus_tag="VKQ27_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88295.1"
                     /translation="MMLEVRDLNAFYGKSHILQGVDLDVGEGEIVSLLGRNGVGRSTT
                     CKAIMGLVAPVGSIRYRGRDIAGLRTNLISRAGIGYVPEDRQVFPTLTVRQNLLLGLK
                     QAGRFGRWNFDDVFRLFPNLAERQNNPAGVLSGGEQQMLTMCRTLMGDPDLILIDEPT
                     EGLAPMLVEQVGRLLAEIARRGVAILLVEQKLTIAMTISQRLYVMGHGRIVFEGTPAD
                     IDATPEIRREWLEV"
     gene            complement(5573..6328)
                     /locus_tag="VKQ27_04870"
     CDS             complement(5573..6328)
                     /locus_tag="VKQ27_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88296.1"
                     /translation="MSAPAAIQLIDVFKSFGATQIIRGVNLQIARGERHAIIGPNGAG
                     KTTLFNLISGRFPITSGTIELNGTRIDGLPPQRINRLGLSRSFQITSIFPRLSVFENL
                     RCALLWSRGYRYSFWHLLPRQRALNEATEHLLADLNLATRRDVPAGLLSYAEQRALEI
                     GMTVAGGASVILLDEPTAGMSHSETEYAVALIRRVTEGKTLVMVEHDMRVVFGLADTI
                     SVVVYGQVIASGPPAEIRASRAVQEAYLGELAA"
     gene            complement(6325..7593)
                     /locus_tag="VKQ27_04875"
     CDS             complement(6325..7593)
                     /locus_tag="VKQ27_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88297.1"
                     /translation="MTRRDWLPWLILALIAAALPWAFYDWSHHRQAGFVVGMLSQMGL
                     MTILALSYNMLLGQAGLFSLCHATFFGFGGYAMVHFLNAAGDGMLPVPMELMPLLAGF
                     ASLGLAIVFGSMVTKQRATAFAMITLGISELVATAALMFHHFFGGEGGVNTDRMINQS
                     LFHFSYASGIQVYYLILAWTFIAAIAMRLLTVTPLGRMANACRDNFERAQFMGYDPRT
                     VRFLQFSLAGFFAGIGGGLYAITYEIVTFDAVASPLSANALLMAYIGGTMVFPGPVLG
                     AVLITLLQSGVSLMSNSWLIYVGVLFIAMVTFAPTGLSGIVVAHQPVWRAGRLSRLLV
                     PYLRLLPAAIASLAGFVGLVELLSFLTIGAEQGKRLVLFGSPVNVASTWPWVTAGALL
                     LVGGIWLVKEARSFSRVWEAVIDDVQEART"
     gene            complement(7590..8537)
                     /locus_tag="VKQ27_04880"
     CDS             complement(7590..8537)
                     /locus_tag="VKQ27_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88298.1"
                     /translation="MEVIVISLLNGLVYGMLLFMLASGLTLIFSMMGVLNFAHASIYM
                     LGAYFAYETSHWIGFWPALIVAPVLCGLIGVAIEVWGLRRVHRNGHVAELLFTFGLVF
                     IIGRSVQMSWGMLPEPYRVPALLDFPLFTLYGLQFPAYRAFMLLVSAAMLGAIWLLLK
                     RTRIGLIIQAALTHPDMVASLGHNVPRVFTIVFAGGCLLAGLAGVIGGNYQSTEPGMA
                     DAMGPIVFVVVVFGGLGSLAGCFIASIIMGMVSTFAVVIDASMADALRPLGLTIQAND
                     LLGEVLTVPVARIGALLPLLMMVLILLFRPRGLMGTRDT"
BASE COUNT         1621 a         2753 c         2832 g         1547 t
ORIGIN      
        1 taccgccgaa cccgaagctg ttcgacaggg cgtagtcgat ccgccggtcc tgcgccgtcc
       61 gcgcgacacg gtcgatcacg ctttcgcggc tgggctcctc aaggttcagg gtggggggcg
      121 ccacattgtc gcgcaccgcg aggatcgaga agatcgcctc gaccgcaccg gcggcaccga
      181 gcaggtgtcc ggtcgccgac ttggtcgacg acatcgccag tcccttggcc gcgtcaccaa
      241 acacatgctc gacggcttcg agttccagat cgtcgccaag tggtgtcgag gtgccatgcg
      301 cattcacgta ttgcacctga tccggggaga tgccgccgtt gcggaatgcc gcgcgcattg
      361 agcgcacggc gccatcatga ccctcggctg gtgcggtgat gtggaacgcg tcgcccgaca
      421 tcccgtagcc ggctacttcg ccatagatct tcgcgccacg tttcttcgcg tgctcgtatt
      481 cttccagcac gagcaccccg gcgccttcgc ccatcacgaa gccatcgcgg tccttgtccc
      541 acggccgcga cgcccgtgtg ggttcgtcgt tgaacgcggt tgacagtgcg cgcgatgcgc
      601 agaacccggc gatgcccagt gcgcacaccg ccgcttccgc accgccggcc accatcacgt
      661 cggcatcgcc ccacatgatc agccgcgatg catcaccgat cgcatgcacg cccgtggcac
      721 aggcggtgac cgctgaatgg ttcggacctt tgagcccata tttgatggac acgtggcctg
      781 acacgagatt gatcagagcc gacgggatga agaatggcga caggcgccgc gcgcggcctt
      841 catgcacctg gatcgaggct tcgtagatcg tctgcagacc gccgatgccg gagccgatca
      901 tcacgccggt ggcgcatttg tcctcttcgg cctcgggcag ccagcccgaa tcctccatcg
      961 cctcggtggc ggcgaccatc ccgagatgga tgaagcgatc catcttcttc acgtctttca
     1021 ccggaatcca ttcggcgagg tccagcccac catcggcctt cataccggca gggatctgac
     1081 ccgcgacctt gcagggcagg tcgttcgtat cgaacgactg gatggagccg attcccgatt
     1141 cgccggcgat caaccgccgc catacgtgct cgacaccaag gcccagggga caggcaatgc
     1201 ccatgcccgt caccacaaca cgccgcatcg cttcgaatcc tcccagggcc gctcaggcag
     1261 ccttctgctt ctcgatgtac tcgatggcat ccttcacggt gctgatcttc tctgcggcat
     1321 cttctgggat ttccacgccg aacgcctctt cgaaagccat caccaactcg acggtgtcca
     1381 gactgtcggc accgaggtcg tcgatgaagg aggcctcggg cgtgaccttc gcttcctcaa
     1441 ccccgagatg ctcgaccaca atctttttta ccttgtccgc gatctcgctc atcggattct
     1501 gaactcctca tgaaggccct gtgcctgccg ccggaccaag attttgggcg cttagcatgg
     1561 tttccgaggg cacgccaacc gcccacggtc ctgctaaacc atggccattc cgccgtttac
     1621 gtggagtgtt gcgccagtga cccagccggc ttcctctgag gccaggtagg cgacggcggc
     1681 tgccacatcc tcgggttttc cgatccgccc aaggggaatc gcgtctgtca gcttggtctt
     1741 ttgctggtcg gaaagcttgt cggtcatggc tgtagcaacg aatccaggag ccaccgcgtt
     1801 gaccgtaatt ccgcgcggcg cgacttcctg tgcgagcgcc ttggtcatgc cgatcaggcc
     1861 ggccttggcg gcggcgtagt tggcctgacc ggcgttgccg gtggagccga cgatggagcc
     1921 aatattaata atgcggccgg cgcggcggcg cagcataccg cgtagcgccg agcggcacag
     1981 gcggaacggc gccgacagat cgacatcgag caccatttgc cagtcagcat cgctcatgcg
     2041 cagcgcgagg gtatcgcgtg tcatgccggc attgttgatc acggcccaga gcgggccaga
     2101 ggcctgttcg gcggcctcga tcagcgaatc gggcgccgag gcgtcgcgca gatccgcggg
     2161 acagacgaag gctcgctcgc ccagttcggc ggtcaaagcc tccagtgcgt cctgccgcgt
     2221 gccggagagc gcgacgacag cgcccagctt gtgcagggtt cgggcaattt ccgcaccgat
     2281 gccacccgag gaccctgtta ccagaacagg tttgccatcc agccggaaca tgctcacagc
     2341 gccttcaaca gggcttcgat ctcggccggt gtggcagccg aggcggtggc ggcgtcgggg
     2401 gcgattcggc gcaccaggcc ggaaagcacc ttgcccgcgc ccagctcgat gaaactgtca
     2461 cagccgagtt ctgccatggc gttcacacac tcgcgccagc ggaccatagc agtgacctgt
     2521 ttcacgagaa gctcgcggat ttcggcggga tcggtggcct tggcggagga aacgttggcg
     2581 accaggggca cgatcggggc gcggggcggg gtgctttcca gggcctcggc catggcgtcg
     2641 gcggcggggg ccataagggc acagtggaac ggggcggaga ccggcagcaa catggcgcgg
     2701 cggacgcctt tggaggcggc gagggctacg gcgcgctcca cggcgccgcg atggccagag
     2761 acgacgattt ggccggcgcc gttgtcgttg gctggctgaa tcacttcggt ctgcggcggg
     2821 tcactttcgg ggacggggcc ggcttcggca cagatcgatt cggcctggga catgtctgca
     2881 cccagcaacg cggccatggc gccttcgccg gcgggcaccg ccttctgcat ggtctggccg
     2941 cggagtttca ggaggcgggc ggcgtcggcg atgtcaaatg cgccggcggc ggcgagggcg
     3001 gagtattcgc cgagcgaatg gccggctacg acgacggctt tctggcgcag ggtgaaaccg
     3061 ccttcctttt ccagcacgcg cagaacggcg agtgagtggg ccatcagggc gggctgggtg
     3121 ttctcggtca gcacgagttc ctcgccaggg ccttcgaaca tcagcctgga gagcttctgg
     3181 cggagggttt cgtcgacttc ctcgaatact tctcgggcgg acgggaaggc ggtggcgagg
     3241 tcgcgcccca tgccaaccgc ctggctgcct tggcctggga agatgaaggc gtgaacaggc
     3301 atggttgttt cccctgtggc gcggcggggt agcggggggt cacgaaagtg tcaatgtgcc
     3361 agcggggcga gtggagggga atatcacgga ttgcgccgat tggctcgcta cgcgagccac
     3421 tgcaccgatt gcaggggatt cttattttaa gccttcagca actcgcaagt tgcagggttg
     3481 tcagatgggt gctgggctgg tgcatggcgg tgatacgacg cgcgtgatta tttgtccggc
     3541 gatgcgggtg catcgtcggc ttgggccagg gctcctcgaa tcggcttatg aggcttgctt
     3601 gtgccatgaa cttcgctgcg cagacctgcc gtttcgcagg caggcaggtc ccgctgcgtg
     3661 ttcgctatga tggcgtgcag cttgatcgcg gctatgtcgc ggacatcatc gtgcgcgacg
     3721 aagtgctgct cgagctgggg tctgtggaac agctgctccg ctctttcagt cgcagctcgt
     3781 gacgtatatg cgtctcgccg gctgccgcgt tggcctgctg atcaacttca acacggactc
     3841 gctgacggac ggccagaagc actgcgtgct ttagaatttc tttcggtgaa atctgtgcgg
     3901 tggccagcag cgcgagccaa tccgcgcaat ccgtgttaga gagcggtggt tgagccgcat
     3961 tggaggttct tcttatgggg caattcgtta tccacacgca tggacgcctg caggagtggg
     4021 tggcccagga gaaaggctac ttcgccgcag aaggcctgac ggactatata ttggcctcgc
     4081 acgggctgct gtcacgcgac caggtcgcga tgcagacacc ggccagcctc gacattcgcc
     4141 atggcgctta ccagagctat gaacaaggcc gtgacgcatc agtcagttgc gcctgccatt
     4201 ggacggtaaa caaggcagcc tcggccgagt tcggccggct gtggggcgaa tgctacacag
     4261 tgacgccttg cggggtgttc gttcccgcca actcggcaat caagtcaccc gaagatctgg
     4321 ccgatgttgc cgtacatgtc ggctatcagt ccggcagcca ttacaccacg atccaggcac
     4381 tcgagccatt cctggagctg gacagtatca agctgcggtt cggcggatcg ccagccgacc
     4441 gggtggatca actgctcgac ggcagcgcgc cggcggccac ggtttttggc gcacagtatt
     4501 atctgatcga gcagcttggc ttccgtaaag tcgtcgatgc cagcttcatg atcgcggcga
     4561 tggtgcctct ccatgtggat acggcggagg tccgcaagta cttcgcggcg ttgcggcggg
     4621 cacagggcga tatcgatgtg tcgcatcaac cgtacacaca ttactacggg aacgagctgc
     4681 cggagcggca tagagcgctg gtcgatgtgc ggcgctttgg tcccggcgag cgattggttt
     4741 tcgagccata tacccaggag atgtatcagc ggacgcaggc ctggatcgat gaacgcggca
     4801 tcttcagcga agggcccagc aatccgcatg gctatgccga gtcggtgatc cggctggagg
     4861 cggcggagta ggcgccctta cacctcgagc cactcgcgcc ggatttcggg ggtggcgtcg
     4921 atgtccgccg gcgtgccctc gaagacgatc cgcccgtggc ccattacata gaggcgctgc
     4981 gatatcgtca tcgcgatcgt cagcttctgc tccaccagca ggatcgccac gccacgccgc
     5041 gcgatttccg cgagcagccg gccgacctgt tccaccagca tcggtgcgag gccctcggtc
     5101 ggctcatcga tcaggatcag gtccggatcg cccatcagcg tgcggcacat ggtcagcatc
     5161 tgctgctcgc cgcccgacag cacgccggcg ggattgttct gccgttcagc aagattcggg
     5221 aacagccgga atacgtcatc gaagttccag cgtccgaatc ggcctgcctg cttcagaccc
     5281 agcagcaggt tctgccgcac cgtcagcgtg gggaacacct gccgatcctc ggggacatag
     5341 ccgatcccgg cgcgcgagat caggttggtg cgcaggccgg cgatgtcacg cccgcgatag
     5401 cggatgctgc cgacaggtgc gaccagaccc atgatcgcct tgcaggtggt ggaacgtccg
     5461 accccgttcc tgccgagcag cgagacgatc tcgccctcgc cgacatcgag atcgacgccc
     5521 tggagaatgt ggctcttgcc gtagaacgcg ttcaggtcgc ggacttccag catcatgccg
     5581 cgagctctcc cagatacgct tcctgtaccg cgcggctggc acggatctcc gccggcggtc
     5641 ccgaggcgat cacctggccg tacaccacca cgctgatcgt gtcggccaag ccgaacacga
     5701 cgcgcatgtc gtgctccacc atcaccagcg tcttgccctc ggtcacgcgg cggatcaggg
     5761 cgacggcgta ttccgtctcg ctgtggctca tgccggcggt tggttcatcc agcaggatga
     5821 cgctggcgcc acctgccact gtcatgccga tctcgagcgc gcgctgctcg gcataggaca
     5881 gcagcccggc gggcacgtca cgccgtgtcg cgaggttcag gtctgccagc agatgctcgg
     5941 ttgcctcgtt cagcgcgcgc tgtcgcggca acaggtgcca gaatgagtag cgatagccac
     6001 ggctccatag cagcgcgcac cgcagattct cgaacaccga caggcgcggg aagatcgagg
     6061 tgatctggaa gctgcgcgac aggccgaggc ggttgatccg ctgcggaggt agcccatcga
     6121 tgcgcgttcc attcagttcg atcgtgcccg acgtgatcgg gaagcgtccg gagataagat
     6181 tgaacagggt cgtcttgcca gcgccattcg ggccgatgat cgcgtggcgt tcaccgcgcg
     6241 cgatctgcag gttcacgccg cgaatgatct gcgtcgcgcc gaaacttttg aacacgtcga
     6301 tcaactggat cgcggcaggg gcgctcatgt gcgcgcctcc tgcacatcgt caatcaccgc
     6361 ttcccatacg cgcgagaagc tgcgtgcctc tttcaccagc cagatgccac caaccagcag
     6421 cagcgcgccg gcagtcaccc atggccacgt gctggctaca ttgaccggcg agccaaacag
     6481 cacaaggcgc ttgccttgct ccgcaccgat ggtgaggaat gacaatagct cgaccaggcc
     6541 gacgaagccg gccagcgatg caattgctgc cggcagcagg cgtagatacg gcacgagcag
     6601 gcgcgacagc cggcccgcgc gccacaccgg ctggtgcgcg acgacgatgc cggacagtcc
     6661 tgtcggcgcg aaggtcacca tggcgatgaa aagcacgcca acatagatca gccaggagtt
     6721 ggacatcagg ctgacgccgc tttgcagcaa cgtgatcagc accgcgccca gcaccggccc
     6781 ggggaatacc atggtgccgc cgatatacgc catcagcaga gcgttcgctg acagcgggct
     6841 ggctaccgca tcgaatgtga cgatctcgta ggtgatcgcg tagagaccgc cgccgatccc
     6901 cgcgaagaaa cctgcaagtg agaattgcag gaagcgcaca gtgcgcggat cgtagcccat
     6961 gaactgagcg cgctcgaaat tgtcgcggca ggcgttcgcc atgcggccga gcggcgtgac
     7021 tgtcagcagc cgcatggcga tggcggcaat gaaggtccag gccaggatca ggtaatagac
     7081 ctggatgccg gacgcatagc tgaaatggaa cagactctgg ttgatcatgc ggtcggtgtt
     7141 gacgccgccc tcgccgccga agaagtggtg gaacatcagg gcggccgtcg cgaccagttc
     7201 gctgatgccc agcgtgatca tcgcaaacgc cgtggcgcgc tgcttcgtca ccatcgatcc
     7261 gaacacgatc gccaggccca ggctggcgaa gccggcgagc agcggcatca gctccatggg
     7321 taccggcagc atgccgtcgc ctgccgcatt gaggaaatgc accatcgcgt agccgccgaa
     7381 gccgaagaac gtcgcgtggc acagcgagaa cagaccggcc tggccgagca gcatgttgta
     7441 ggacagtgcc agtatggtca tcaggcccat ctggctgagc atgccgacga cgaagcctgc
     7501 ctgccggtga tgcgaccaat catagaacgc ccagggcagc gccgctgcga tcagggcgag
     7561 gatcagccag ggaagccagt cgcgccgagt cacgtgtcgc gtgtccccat caggccgcgc
     7621 gggcggaaca gcaggatcag aaccatcatc agcaacggca gcagggcacc gatgcgcgcc
     7681 actggcacag tgaggacttc tccaagcagg tcgttggcct gtatcgtcag gccaagcggg
     7741 cgcagcgcat ctgccatcga ggcgtcgatc accacggcga aggtcgagac cattcccatg
     7801 atgatcgagg cgatgaagca tcccgccagc gatcccaacc cgccgaacac gacgacaacg
     7861 aaaacgatcg gccccatggc gtccgccatg ccgggttccg tggactggta gttgccgccg
     7921 atcactccgg cgaggccagc cagcaggcag ccgccggcaa atacgatcgt gaatacacgc
     7981 ggcacgttgt ggccgagcga agcgaccatg tcggggtggg tcagtgcggc ttggatgatc
     8041 agcccgatgc gggtgcgctt cagcagcagc cagattgcgc cgagcatcgc ggcagagacc
     8101 agcagcatga atgcgcgata ggctggaaat tgcagcccgt acagggtgaa cagcgggaaa
     8161 tcgagcaggg cgggcacgcg gtagggctcg ggcagcatgc cccaactcat ctgcaccgag
     8221 cggccgatga tgaatacgag gccgaatgtg aacagcaact ccgcgacgtg gccgttgcgg
     8281 tgaaccctgc gcagacccca tacctcgatt gcgacgccga tcaggccgca cagcacgggc
     8341 gccacgatca gggccggcca gaatccgatc cagtggctgg tttcgtaggc aaaatacgcg
     8401 ccaagcatgt agatcgaggc atgtgcgaaa ttcagcacgc ccatcatgct gaatatcagc
     8461 gttagccccg acgccagcat gaacaacagc atgccataga cgaggccgtt caacagggag
     8521 atgacgatga cctccatgcg gaacgacctc gttgcggcgc gtggtagacg ggccttactg
     8581 ggttggccgc ttcatcttgc agctagtggg aagggtcagg tcggaggcag agagcctcgc
     8641 ctcctctttc cagccgaggc cggtatgctc ggcgtcgagc ttcgcgccgc gggtgaagat
     8701 cgcctcgcgc agagggctga gcagctgatg atccccggcg cgcatgatgt tct
//
LOCUS       DATGEM010000156         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1439397, whole genome shotgun sequence.
ACCESSION   DATGEM010000156 DATGEM010000000
VERSION     DATGEM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439397"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VKQ27_04885"
     CDS             <1..1149
                     /locus_tag="VKQ27_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HLY88299.1"
                     /translation="ACTLNIDDNPVTIKTIECAIVDRAWEEGWIEPHLPAHRTGKRVA
                     VVGSGPAGMACSQQLARAGHTVTLFEKNDRIGGLLRYGIPDFKMEKHLIDRRIEQMTA
                     EGVVFRTGIEVGVTLPMEALQRDFDAVVLSGGAEWPRDLEVPGREIDDGIHFAMDFLT
                     QQNKRGAGDDEARATRGANTITAKGKHVVVIGGGDTGSDCIGTSVRQGAVSVTQLEIM
                     PKPPERENKALTWPDWPLKLRTSHAHEEGADRDWAVLTKRALGENGKVEALECVRVEW
                     VKGADGRFAMQEVPDSTFNLKADLVLLAMGFLGPHKSGMLEQARVALDPRGNVLANTD
                     DYRTSVDKVFAAGDMRRGQSLVVWAIREGRQCARAVDEYLMGTSTLPR"
     gene            complement(1258..>2535)
                     /locus_tag="VKQ27_04890"
     CDS             complement(1258..>2535)
                     /locus_tag="VKQ27_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HLY88300.1"
                     /translation="RHVLGFEEFMGSAREWPVARAAEACGLRADEITTFARWLADADP
                     LVFAPGNGLERGRNGGSSIRALIALPALLGRLGNGSGLVLGASSAFPKTPAKLQRPDL
                     VPPDTRTLNILDVGRHLAEDDIDPPLRAVFIYNHNPLVVHPDQNRMRRGLARDDVFTV
                     GLDVAMNESMAYCDIVLPGATHFEYADLYASYGHHWLQRAEAVIPPLGDSLPNTEIFR
                     RLATRFGFNDPCFAASDAALMDDAVELGGGVRPSAISTGRATQMRGKDGSELVLFENV
                     FPATPSGRIELRSDTLATRWGEGALMPTWRPRVSDYPLMLISPASDKRISSTLGFDKD
                     APRLMMHPRDAAARSLTDGAEVKVWNERGDVILPLDISDAVPPGVVASEKGAWINTSR
                     TGQTISALVSADMRADLAEGACFNDTMVDVAMA"
BASE COUNT          465 a          816 c          828 g          426 t
ORIGIN      
        1 gcttgcacgc tgaacatcga cgacaacccc gtcacgatca aaacgatcga gtgcgcgatc
       61 gtcgaccggg cgtgggagga aggctggatc gaaccgcatc tgccggcgca tcgcaccggc
      121 aagcgtgtcg ccgtggtggg ttcggggccg gccggcatgg cctgctccca acaactcgcc
      181 cgcgccggcc atacggtaac gttgttcgag aagaacgacc gtattggtgg gttgctgcgc
      241 tacggcattc ctgacttcaa gatggagaag catctgatcg atcgccggat cgagcagatg
      301 actgccgagg gcgtggtgtt ccgcaccggc atcgaagtcg gcgtcacttt gccgatggaa
      361 gccctgcaac gtgatttcga cgcggtggtg ctgtctggcg gcgccgaatg gccgcgcgat
      421 ctggaagtgc cgggccgcga aatcgatgac ggcatccatt tcgccatgga tttcctcacc
      481 cagcagaaca agcgcggcgc cggcgatgac gaggcgcgcg caacgagggg cgcgaatacg
      541 atcaccgcca agggcaagca tgtcgtggtg atcggtggtg gcgacactgg ctccgactgt
      601 attggcacct cggtgcgtca gggcgcggtc tccgttacgc aactggagat catgccgaag
      661 ccgcccgagc gcgagaacaa ggcactcacc tggccggact ggccgctgaa gctgcgcacc
      721 tcgcatgcgc acgaggaagg cgcggaccgt gactgggctg tgctgaccaa gcgcgcgctg
      781 ggcgagaacg gcaaggtgga ggcgctcgag tgcgtgcgcg tagagtgggt gaagggcgcc
      841 gatggccgct tcgcgatgca ggaagtgcct gacagcacct ttaatctgaa ggccgatctg
      901 gtgctgctgg cgatggggtt cctggggccg cacaagtcag ggatgctgga acaggcgcgc
      961 gtggcgcttg atccacgtgg caacgttctg gcgaacaccg atgattatcg cacatcggtc
     1021 gataaggtct tcgcggccgg cgacatgcgg cgtggccagt cgctggtggt ctgggcgatc
     1081 cgcgaaggac gtcagtgtgc ccgcgcggtg gacgagtacc tgatggggac gagcacgcta
     1141 ccgcggtagg gcgcttcccg tcgtcatggc gggcggagac ctgccatcct cttctttgct
     1201 atgtccagcg agagaagacg tggatgccga ccttcgtcgg catgatgtcc agccggatca
     1261 ggccatcgcc acatcgacca tcgtatcgtt gaagcaggcg ccttccgcca ggtcggcacg
     1321 catatcggcc gagaccagcg cggaaatcgt ctgcccggtg cggctggtgt tgatccacgc
     1381 gcctttctcg ctggcaacca cgccaggcgg cacagcatcg ctgatatcga gcggcaggat
     1441 cacatcgccc cgctcgttcc acaccttcac ctcggcgcca tccgtcaggc tgcgtgccgc
     1501 ggcatcgcgc ggatgcatca tcagccgagg cgcgtccttg tcgaagccaa gcgtggacga
     1561 gatgcgcttg tcagaggccg gtgagatcag catcagcgga taatcgctga cccgcgggcg
     1621 ccatgtcggc atcagtgcgc cctcgcccca acgcgtggcc agcgtatcgg accgcagttc
     1681 gatccggccc gaaggcgtgg ccgggaatac gttctcgaac agcaccagct cgctgccgtc
     1741 cttgccccgc atctgggtcg cgcgcccggt cgatatcgca ctggggcgca caccgccgcc
     1801 cagctcgact gcgtcatcca tcaacgcggc atccgacgcg gcgaagcagg gatcattgaa
     1861 gccaaaccgc gtcgccagcc gacggaagat ctcggtgttg ggcagcgaat cgccaagcgg
     1921 cgggatcacc gcctcggctc gctgcaacca gtgatgtcca tacgaagcgt atagatcagc
     1981 gtattcgaaa tgcgtcgccc ccggcagcac gatgtcgcaa tacgccatgc tttcgttcat
     2041 cgcgacatcc agtccgacgg tgaacacgtc atcacgcgcc aggcctcggc gcatgcggtt
     2101 ctggtccgga tgcaccacca gtggattgtg gttgtagata aataccgccc gcagcggcgg
     2161 gtcgatgtcg tcctcggcca gatgccggcc cacatccagg atgttcagcg tgcgtgtgtc
     2221 tggcggtacc agatcgggtc gctgcagttt cgccggcgtc ttgggaaacg cgctgctcgc
     2281 tcccagcacc aacccactgc cattgccgag ccgcccgagc agcgcaggaa gcgcaatcag
     2341 cgcgcggatc gagctaccgc cattgcggcc tcgctccagc ccgtttcccg gcgcaaacac
     2401 cagcggatca gcatcggcaa gccaccgtgc gaacgtggtg atctcatccg cgcgcagtcc
     2461 gcacgcctcg gcggcacgcg cgaccggcca ctcccgagcc gaacccatga actcctcgaa
     2521 gccaagcacg tgccg
//
LOCUS       DATGEM010000157         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1323498, whole genome shotgun sequence.
ACCESSION   DATGEM010000157 DATGEM010000000
VERSION     DATGEM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323498"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VKQ27_04895"
     CDS             complement(<1..1068)
                     /locus_tag="VKQ27_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY88301.1"
                     /translation="MLTRREAARALIAATATVALGKPALAADKVVKIAMDLSLTGADS
                     EGAIRVKNGIMMAIDGCNEGNGIPGIRLEPLVLDDGTPTAGQYDPAQAATNARKMASD
                     PDVVASLGPEMSGAGKAMAPILSMADLATITPSSTNPDITDPKFAQQFRPGGKPIYFR
                     TVTTDAYQGPNMANFYADVLHVKSVYVLDDSGAYGVGMADAFQKQAEKKGMKVVGRDR
                     LDPKGADYTAVLTKIKSLAPDAIYYGGVGQAGTKLAKQSYDIVPSMIKGGGDGVYGPT
                     MLTSVGFPAANGWYATIASPHTVADNKAQAFVDAYFRKYGKAADDYTITAYDATLVVI
                     DAIRRVAVSGKPINRSAVRDAI"
     gene            complement(1226..>2452)
                     /locus_tag="VKQ27_04900"
     CDS             complement(1226..>2452)
                     /locus_tag="VKQ27_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY88302.1"
                     /translation="RRATAQLLATTAVATAFGGATALAADNVVKVGVDLSLTGADSLG
                     ANRVKNAINMAFDAANQGGAVPGYQFQLLVLDDGTATAGQYDPAQAATNARKMVADPA
                     VMASIGPQMSGAGKAMTPILSQGGLATITPSSTNPDITDPKFAAQYRPAGQPIYFRTV
                     TTDAYQGPNMANFLAEKLHVKNVYVLDDSGAFGVGVADSFEREAKAKGMNVLGRDRLD
                     PKAADYTAILTKIKSMNPDAFYYGGVTQAGVKLAKQAYDIIPKVIKAGPDGMYSSDLL
                     NGAGFPACEGWYTSIAAPHLVADTKAAEFIKAYTGKFSDAPDDYTITAYDASLVIIDA
                     VKRVVAAKKAVTRANVREAIQTAKVPTIQGMVSFDANGDLADRTVSVFQIRQDKSKPP
                     TDVSAQYKYLEVAPQS"
BASE COUNT          425 a          830 c          693 g          504 t
ORIGIN      
        1 gatcgcgtca cgcacggcag aacggttgat tggcttgccg ctcaccgcca cccgtcgaat
       61 cgcatcgatc accaccaaag tcgcgtcata cgcagtgatc gtatagtcat ccgccgcctt
      121 gccgtatttg cggaaatatg catcgacgaa agcctgcgcc ttgttgtcgg ccaccgtgtg
      181 cggcgaggcg attgtcgcgt accagccatt cgccgccgga aaaccaaccg acgtcagcat
      241 tgtcggacca tacacgccat cgccgccacc tttgatcatg ctcggaacga tgtcgtagga
      301 ctgcttcgcc agcttggtgc cagcctggcc gacaccgccg taatagatcg catcgggcgc
      361 caacgatttt atcttggtca gcactgccgt gtagtcggcg cccttcggat ccaggcggtc
      421 gcggcccacc accttcatgc ccttcttttc cgcctgcttc tggaacgcat cggccatgcc
      481 aacgccatag gcaccgctgt catccagcac gtagaccgac ttcacatgca gcacgtctgc
      541 gtaaaaattg gccatgttcg gcccctggta cgcatccgtg gtcaccgtgc ggaagtagat
      601 cggcttgccg cccggccgga attgctgcgc aaatttcggg tcggtaatgt ccggattggt
      661 ggaagacggc gtgatcgttg cgagatcggc catgctcagg atcggtgcca tcgccttgcc
      721 cgcgccactc atctcgggac cgagcgaggc caccacgtcc gggtccgacg ccatcttgcg
      781 cgcattggtc gccgcctggg ccggatcgta ttgcccggcc gtcggcgtgc cgtcatccag
      841 caccaacggc tccagcctga tgcccggtat cccattacct tcgttgcagc cgtcgatcgc
      901 catcatgatg ccgttcttga cccgtatcgc tccttccgag tcagcaccgg tcagcgacag
      961 gtccatcgca atcttgacca ccttgtcggc cgcgagcgcg ggttttccca acgccaccgt
     1021 agcggtcgcg gcgatcaaag cccgcgcggc ttcgcgtcgt gtcagcatgt gaagtccccc
     1081 tgttggtctc gttgcttggc ttggtatttt cacttgccac agaaagcaag ttccatgccc
     1141 atcggaaacc gatgagcaaa aatcagtccc cctcccgggc aggaggggga cgggtcagtt
     1201 caatccatca gcacaggccg cattgttacg attgcggcgc aacttccagg tacttgtact
     1261 gcgccgaaac atcggttggc ggcttcgact tgtcctgcct gatctggaac acgctgaccg
     1321 tccggtccgc cagatcgcca ttcgcatcga acgacaccat gccctggatc gtcggcacct
     1381 tcgctgtctg gatcgcctcg cgcacgttcg cgcgcgtcac cgccttcttc gccgcgacca
     1441 cgcgcttcac cgcatcgatg atcaccagcg acgcatcata cgccgtgatc gtgtagtcat
     1501 ccggcgcgtc gctgaacttc cccgtatacg ccttgatgaa ttccgcagcc ttggtgtcgg
     1561 ccaccagatg cggcgcggcg atcgaggtat accagccctc gcatgccggg aacccggcac
     1621 cgttcagcag atccgagctg tacatgccgt ccggcccggc cttgatcacc ttcggaatga
     1681 tgtcataggc ctgcttcgcc agcttcacgc ccgcctgcgt cacaccgcca taatagaacg
     1741 cgtccggatt catcgacttg atcttcgtca gaatggccgt gtagtcggcc gccttcggat
     1801 ccaggcggtc acggccaagg acattcatcc ccttcgcctt cgcctctctc tcgaacgagt
     1861 cagccacgcc cacaccgaac gcgccgctgt catccagcac gtagacattc ttcacgtgca
     1921 gtttttcggc gaggaagttc gccatgttcg gcccctggta ggcatcggtc gtgaccgtac
     1981 ggaagtagat cggttgaccc gccggccgat actgcgccgc gaatttcggg tcagtaatgt
     2041 ccgggttcgt ggaagacggc gtgatcgtcg ccagcccgcc ctggctcagg ataggtgtca
     2101 tggccttgcc ggcgccgctc atctgcggac cgatcgaggc catcacggca ggatccgcca
     2161 ccatcttgcg ggcattcgtc gctgcctgcg ccggatcata ctgcccggcc gtcgccgtgc
     2221 cgtcgtcgag caccagcagc tggaattggt aacccggaac cgcacctccc tggttcgcgg
     2281 catcgaacgc catgttgatg gcattcttga cccggttggc tcccagcgag tccgcgccgg
     2341 tcagcgacag gtcgacgccg actttgacga cgttgtcggc cgcaagcgca gtggcgccgc
     2401 caaatgccgt agcaacggct gtggtggcaa gaagctgggc ggttgcccgg cg
//
LOCUS       DATGEM010000158         4706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_528477, whole genome shotgun sequence.
ACCESSION   DATGEM010000158 DATGEM010000000
VERSION     DATGEM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528477"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VKQ27_04905"
     CDS             <1..646
                     /locus_tag="VKQ27_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLY88303.1"
                     /translation="LIGVQETRQLLTGAEAHWGELVREALRVAPMQRLADLFRRLLDE
                     GLTLRNLRGVLESVLEHGAREQDPVMLAEAVRASMRRQVCHAYADPLRVIGAFIVDAG
                     AEAVLRSAVQQAGNSAHLPLAEGTATALVERVRAEVSGTRGPGPVLLTAMDVRRHLRA
                     LLHNSGIHTPVLSFHDLLPEFTVQPLGTIRLSDAPGAAVVQASPARAVEAMAAA"
     gene            643..1965
                     /locus_tag="VKQ27_04910"
     CDS             643..1965
                     /locus_tag="VKQ27_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HLY88304.1"
                     /translation="MIRLVLVLLGMCLQAAMPCPAQTVQGRGTVTLNVGEGRLLRLDR
                     TVNHVLVGDSAIADVQVVSPQAVYVYGRAPGDTTLTATDSSSGVAAQMSLRVQRSALA
                     AQAALPAGASIALRFEGNRMVVSGPVSNLGQAMEVEGTARAYNPDRLPPLDMTRLAGA
                     QQVTLRVRIAEVSRTKLQQLGLNLSVLANPGSFTFNLLTGTFLGSGATGGVSSSLING
                     LGNAPNAPNFANATVGINGARVNAQALLNALESEGVLTTLAEPNLTTISGETANFLAG
                     GEVPIPVPQSFGVTTIEYKRFGVQLAFTPTLLPGDRIAMRVRPEVSQISNANEVNING
                     TVVPSFITRSTETNVEMASGQTLAIAGLFQRTEQNTLQKFPGLGDLPILGALFRSTAF
                     QRDQTELVILITPYLTQPVSSPSAYTLPTDTPNGAPAAPEIASGFVAN"
     gene            complement(2560..3369)
                     /locus_tag="VKQ27_04915"
     CDS             complement(2560..3369)
                     /locus_tag="VKQ27_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88305.1"
                     /translation="MVRLGDDMRRNGDLAGALAIYRSAERSDQHDSIPLERMGETLLA
                     LGDAARAEQAFRAALTLAPDDGNATRGLAQALLAGQHADEALPILRGLAAGSSDPRLL
                     RAYGTALDLTGQSEAAQLVYRRGLQIAPADADLHGNLALSLAISGDTQGALQQMRAAI
                     DAPDPDPRQEANAVLIMVLAGQADAARERGDRALGAAATEVLLGRAAQARAATDARGR
                     ALALGVITATPAVAPGQPQPMAPATAGPSAPRLAASPNSSLSPAEPPAAAR"
     gene            complement(3596..4249)
                     /locus_tag="VKQ27_04920"
     CDS             complement(3596..4249)
                     /locus_tag="VKQ27_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HLY88306.1"
                     /translation="MCDQENAIGTWICENAHRSEERFPTIDATAYVAPTATICGDVVI
                     GPNTYVAFGAVLTAEGGSIRIGAECVIREQAVIRATQKHNTVIGNHVLIGPHAYLVGC
                     VVGDCAFLATGANAFHGANIGARAEVRIYGTVHVQTRLPPDTEMPIGWVAAGDPAHIL
                     PPDRHDEIWKILGPLNFPDVGYGIDRPASGGMDLRGITRTVTRELRQRHERDVVRMS"
BASE COUNT          800 a         1502 c         1558 g          846 t
ORIGIN      
        1 gctcattggc gtccaggaaa cccgtcaatt gctgactggc gccgaagcgc attggggtga
       61 gctggtgcgc gaagctctga gggtggcgcc gatgcagcgg cttgccgacc tgttccgccg
      121 gctgctcgac gaggggctga cactgcggaa cctgcgcggt gtgctggagt cagtgctcga
      181 acatggagcg cgggaacagg atccggtcat gctggcggag gcggtgcgcg ccagcatgcg
      241 acgacaggtc tgccacgcct atgccgatcc attgcgggtg atcggggcct tcattgtcga
      301 tgccggagca gaggcggtac tgcgcagcgc cgtgcagcag gccgggaaca gtgcgcatct
      361 tccattggcc gagggcacgg cgaccgcgtt ggtggagcgc gtgcgcgccg aggtcagcgg
      421 cacacgcggc cccggcccgg tgctgctcac ggcgatggat gtgcgccgcc acttgcgggc
      481 attgctgcac aacagcggca tccatacgcc ggtcctgtcg ttccacgacc tgctgccgga
      541 gttcaccgtg cagccgcttg gcaccatccg gctgtcggac gcgcctggcg ccgccgtcgt
      601 gcaagcgtcg ccggcgcgcg ccgtcgaagc gatggccgcc gcatgatacg tctcgtgctt
      661 gtgctgttgg gcatgtgctt gcaggcagcg atgccctgcc ccgcgcaaac agtacaggga
      721 cgcggcacgg tcacgctgaa tgtcggcgaa ggacggctcc tgcggctcga ccggaccgtc
      781 aaccatgtgc tggttggcga cagcgcgatc gccgacgtgc aggtcgtgtc accacaggcg
      841 gtctacgtgt acggccgggc gcccggcgat accaccctga ccgcgactga ctcaagcagt
      901 ggggtggccg ctcagatgag cttgcgggta cagcgcagcg ccctggccgc gcaggctgcc
      961 ttgccggccg gtgcatcgat tgcgttgcgg ttcgaaggca atcgcatggt ggtcagcggg
     1021 ccggtcagca atcttggcca ggcgatggaa gtggaaggca cggcgcgcgc atacaatccg
     1081 gatcgactgc cgccactgga catgacccgc cttgccggtg cgcagcaggt cactttgcgc
     1141 gtgcgcattg ctgaagtgtc gcggaccaag ctgcagcagc tcgggttgaa cctgagcgtg
     1201 cttgcgaatc cgggcagctt caccttcaac ctgctgaccg ggacgttcct cggctccggc
     1261 gcgacaggcg gcgtgagctc cagcctgatc aacggacttg gcaacgcacc gaacgctccg
     1321 aacttcgcca atgcgacggt gggaatcaac ggcgcgcgcg tgaacgcaca ggcgttgctg
     1381 aacgcgctgg agagcgaggg cgtcctgacc acgctggcgg agccgaacct gacaacgatc
     1441 tccggtgaga ctgcgaactt cctcgctggc ggcgaggtgc cgataccggt gccgcagtcg
     1501 ttcggcgtca ctacaatcga atacaagcgt ttcggcgtgc agcttgcgtt cacgccaacg
     1561 ctgcttcctg gcgaccggat cgccatgcgc gtgcggccgg aggtcagcca gatcagcaac
     1621 gcgaacgaag tgaatatcaa tgggaccgtg gtgccgtcct tcatcacgcg cagcactgag
     1681 acgaatgtgg agatggcgag cggtcagaca cttgccattg ctggcctgtt ccagcgcacc
     1741 gagcagaaca cgctacagaa attccccggg ctcggtgact tacccatcct gggtgcgctg
     1801 ttccgctcca cggcctttca acgcgaccag acagagctgg tgatcctgat cacgccctat
     1861 ctcacgcaac cggtgtcctc gcccagcgca tatacgctgc caaccgacac gcccaatggc
     1921 gcgcccgccg cgccagagat cgcatctggc ttcgtggcaa attgagaatg atcctcgggc
     1981 gattgcttct ggcgtctgga ctggtcttgc tgtccggctg cgtgcatcag gcgcctccca
     2041 aggtctcgca gtatcgttcc gccacggtcg cgccgccgct ggcactgcgt tatgcccccg
     2101 gtgcgattaa gctagcgccg gatgatgtgg tgcgggtgcg ggcgctgggt ggcgccctgc
     2161 cggccggtgc ggtgccgcgc ctcatcgccg gcggcccgct gttgtcgcaa cgggcgcagg
     2221 ctatggaggc gttgctggac cgtccggtgg agattcaact cgctggagca acggcggctg
     2281 atccggacag cgcattgctg ataccgcccg cgccgcctgc catcattgcc acgccctgcc
     2341 tgggacaggg cgaaaggctg gttggcgata tgtggccagg caatgacgcg gagcaggctc
     2401 ggctcacgcc gcctgggtgc gcaactgcaa cagcgatcgc cgcgcaggtg gtggcaccgc
     2461 aggacctgct gcagggacgg ccgttgcctc cgggtgcggc gagcccattt gtcagcgcga
     2521 tcgagcgcta ttatcggcgc aatgaaaccc gggcgaacac taccgcgcag ccgcaggagg
     2581 ctcagcaggg gacaggctcg agttcggcga cgccgccagt cgcggcgctg atgggcccgc
     2641 tgtcgcaggt gccatcggct gtggctggcc aggcgccacc gccggcgtcg cagtgatcac
     2701 tccgagagca agcgctcgcc cgcgcgcatc tgtggcagca cgcgcctgag cggcgcggcc
     2761 gagcaagact tcggtagcag cagcgcccag cgcgcggtcg ccacgctcgc gcgctgcgtc
     2821 ggcctggccc gcaagcacca tgatcagcac ggcgttcgcc tcctgcctcg gatccggatc
     2881 gggtgcatcg atcgccgcgc gcatctgctg caatgcgcct tgtgtgtcgc cgctgatcgc
     2941 cagcgacaga gccagattgc cgtgcaagtc ggcatcggcc ggcgcgatct gcagtccccg
     3001 ccgatagaca agttgcgctg cctcggactg gcctgtcagg tcgagtgccg ttccatatgc
     3061 gcgcagcagc cgcgggtcgg aagagccggc cgcaagcccg cgcagtatcg gcagcgcctc
     3121 gtcggcgtgc tgccctgcca acagggcttg cgcgagacca cgggttgcgt tgccgtcgtc
     3181 cggcgccaga gtgagcgcgg cgcggaatgc ctgttcagcg cgcgcggcat cgccgagggc
     3241 gagcagggtc tcgcccatcc gttccagcgg gatggagtca tgctggtcgc tccgttctgc
     3301 cgaacgatag atcgcgagcg cgccggccag atcgccgttg cggcgcatgt cgtcgccgag
     3361 ccggaccatc gagggtgccg tgttggtcgc actatgcggg tccgtgacag gtttgggaga
     3421 cgtgcaaccc gcaagcccta aaagagctgc cgcaagggct gggcgcagat cgtgcggcat
     3481 gtattctccg gagagctgag aacacaggtc ttacgacagg cgcccgacga agtcgcgtgg
     3541 tgcggccgct cggacttgag ccaggggcat cgacacgcga gcacctctgg gccgactagg
     3601 acatgcggac cacatcgcgt tcgtgccgct gccgaagctc tcttgttaca gtccttgtaa
     3661 tgcccctaag gtccataccg ccagacgccg gccggtcgat gccatatccc acgtctggga
     3721 aattcagcgg cccgaggatt ttccatattt catcatggcg atccggcggc aggatgtgcg
     3781 cggggtcccc tgcagcaacc cagccgatgg gcatttcagt atctggtggc aaacgtgtct
     3841 gcacatgcac cgtgccgtat atacggacct cggcgcgagc accaatgttg gcgccgtgga
     3901 atgcatttgc gcccgtggcg aggaatgcgc agtcgccaac cacacagccg acgaggtaag
     3961 cgtggggacc gatcagcacg tgattgccaa tcacagtatt gtgcttctgt gtcgcacgaa
     4021 tgacagcctg ctccctgata acgcactctg cgccgatccg tattgagccg ccttcagcgg
     4081 tcagcacggc accgaaggcc acgtacgtat tggggcctat cacgacgtca ccacagattg
     4141 tggctgtcgg cgcgacgtag gcggttgcat cgatggtcgg gaaccgctct tcacttcgat
     4201 gagcattttc gcaaatccat gttccgattg cattctcctg atcgcacaaa cgctgatgcg
     4261 atctgtccgg tttcccctcc cctggaaatg ctgttggggc ttcgcgccga ccttgtcgcg
     4321 aatcgcattt cgtattgcga ggctatgaca ctgacccaca gcgaaaccga cctgcctaca
     4381 atcagcacgg gcgatgtagt catgcccaca ggtgatgctg gaccggcaaa tgcaatcaaa
     4441 ttggctcgat tcaccctggg cgagcaaacg gtctcttgca gcgacagtct ggtgaagccc
     4501 cttcgctttc ccccttatgt tcattcttcg tcgagtgcaa tgcataggat gaggttcggc
     4561 ggagcgagga ggtaaaggca gccggagccg agctgatgcc tgctgccaat tacggtttca
     4621 gcactctgtt tccgtgagtg tgtgaccggt tcggcgtgtc gttacagccg aaccactcgc
     4681 agctggccgt tatgtcggcg gtcacc
//
LOCUS       DATGEM010000159         6274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1439242, whole genome shotgun sequence.
ACCESSION   DATGEM010000159 DATGEM010000000
VERSION     DATGEM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439242"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VKQ27_04925"
     CDS             <1..373
                     /locus_tag="VKQ27_04925"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88307.1"
                     /translation="RASLDELAEAFHERHAQTYGHANRAERVQLVNLRVTALGRQPDL
                     VLAQQADAAKARKRSREVWFADGGFLATPVHWRDGLMPGTEIAGPAIIEAMDSTTVIP
                     PHWQARIDGQGYMRISTGRVA"
     gene            373..2073
                     /locus_tag="VKQ27_04930"
     CDS             373..2073
                     /locus_tag="VKQ27_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY88308.1"
                     /translation="MARLKTDPARFEVVKNALTCAAEEMKIVLAKTAYSPLLKVAGDY
                     SCGIFDIRGNMVAQGPDLPIHLGSMPDAVRAVVVAFPDVAPGDVFIHNDPYQGGSHLP
                     DVNVVAPAFHEGRLLGFGCVRAHWPDIGSATPGSYGAVTEIYGEGLRLPPIRLYRDGK
                     PDPAIEAIIFANVRTPTERLGDLRAQVSANWRAAARLQALAAKYGTDALLEIMQEVQD
                     YSETMMRAALRALPDGEAEFTDVFDGDGVIAPGETDDETFTVKLRITKRGDTITADFA
                     GSDPQVAGPMNAPLTVTASGVFCALKMIADPKSLIPPNSGCWRPVTVTAPPGSVVNAQ
                     LPAPVVYANHEMSHRVADMVMAAMFAFTPRTVMAGSQGTSAVMTFGGTDYRTDERYVS
                     YESVKGGFGARPVKDGINAVASTVSNMMNTPIEIVEMSFPLRVEEYALVQDSGGAGTW
                     RGGLGVRRVWRVLERQAHAAVCCERTVTPPFGLAGGQAGAAAKLELIPPHGNARRLNS
                     KGGFIAPADSLVVIEAPGSGGYGPPAERDRASLSEDLKDGYVSAAAARRDYGVVAAGE
                     "
     gene            2086..2331
                     /locus_tag="VKQ27_04935"
     CDS             2086..2331
                     /locus_tag="VKQ27_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HLY88309.1"
                     /translation="MSGAQPTIAQKSPYRMEVEAGKSYWWCACGKSASQPFCDGSHRG
                     SGFTPIEYKATEDGTIPFCGCKMSNGKPRCDGTHRSL"
     gene            2356..3462
                     /locus_tag="VKQ27_04940"
     CDS             2356..3462
                     /locus_tag="VKQ27_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLY88310.1"
                     /translation="MKIDDVSLTIFTWDGIPATNYHTGTATAGNSNLGLLRIHTDEGL
                     EGHAFLGSATNPASMDGPQLIRSLKPMLMGQNPLERERLHGAMRLISRAVSYRTIGAV
                     DTALWDLGGKIAKLPVHALMGTTRTSIMGYASSQVLPDIAAYVDQAQTFKANGWRAYK
                     IHPPRDPEQDIKVCQAVRKAVGDDFRVMLDSTWSYNYVDALRVGRAIEELGFYWYEDP
                     MSDEDIYNYVKLREKLDIPIMATEFPAGGLETYPIWLTERATDYLRGDIPNKGGLTTM
                     LKTAHLAEAFSLKYEIHHSGNSLNNLANLHLCMAIRNTTMFEVLLPDGAHKYGMAKEL
                     EIDRDGMLHAPTGPGLAGEIDFELIKRKTEAVLS"
     gene            complement(3500..4900)
                     /locus_tag="VKQ27_04945"
     CDS             complement(3500..4900)
                     /locus_tag="VKQ27_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLY88311.1"
                     /translation="MTQVVVIGAGPAGLVAATLLAEQQVPVLLLDEQPAPGGQIYRNI
                     ECAVAKPALAVALGADYQYGAQLTARFRASGATYQPGAQVWQVTPQREVWFSHGGRSQ
                     QVVGDTIIVATGAMERPVPLPGWTLPGVMTAGALQIMLKSAALVPDEPFVIAGSGPLL
                     YLLVQQCLATGARPAAVLDTTARANQQRALRFLPKAAATREGRAYLAKGLAMLAALRR
                     SGVPRYRHVTDIRIDGTASVTGIAFRSGGRQHRLATRLVALHEGVIPAQQLPRSIGCD
                     FVWDSVQCCFAPRLDAWGNSSIDGVLIAGDAGGIGGARAAEHAGRLAAYEALRHLGRI
                     NVTQRDSLAANDQRARHAHLAIRRFLDVLYAPRDEILQPADDVTVCRCEEIAAGAVRA
                     VVRQGCLGPNQAKAFLRAGMGACQGRLCGPVVTELIAAERGVAPGEVGYYRIRPPLKP
                     ISLGELVGCDSRHGRA"
     gene            complement(4897..5187)
                     /locus_tag="VKQ27_04950"
     CDS             complement(4897..5187)
                     /locus_tag="VKQ27_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY88312.1"
                     /translation="MWRRLEPPAEAIRFDYEGRAITARPGDTVAAALIAAGETTLRMT
                     AVSNSPRAPYCMMGVCFECLMEIDGVGNRQACLTPATEGMQVRRQRGARGVL"
     gene            complement(5171..>6274)
                     /locus_tag="VKQ27_04955"
     CDS             complement(5171..>6274)
                     /locus_tag="VKQ27_04955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88313.1"
                     /translation="MAYDLIVIGGGLVGGAIAWGAAREGASVALIDQGDIAFRAARGN
                     FGLVWVQSKGAGLPAYAHWTRTSAELWPQLSSAMAESTGIDVALKQNGGLAFCLSDKE
                     FEDRAALLHRMHNESGDIGTRMIDRAEVQAMVPGLGDRVVGASYCPIDGEAHSLYLLR
                     ALHAALLKLGGEYLPGRTVDAIDAAPNNFTVRIGAEHLTAAKVILAAGLGSRELAPMV
                     GLSMPVVPSRGQIIVTERVRPFLDYTCHTLRQTIEGTVMLGDSQEDAGFDTSIGVPVL
                     QDIANRNLAVFPALKDATIVRTWAALRVMSPDGFPIYEQSQRYPGAFAATCHSGVTLA
                     GAHALALAPAIMAGELPERFDAFNSRRFDVAKA"
BASE COUNT         1101 a         1858 c         2253 g         1062 t
ORIGIN      
        1 acgggcgtcg ctggatgagc tggccgaggc gttccacgag aggcatgcac agacgtatgg
       61 gcatgcgaac cgcgccgaac gggtgcagct ggtgaatttg cgcgtcaccg cgcttgggcg
      121 gcagcctgat ctcgtgctgg cgcagcaggc cgatgcggcg aaggcgcgga agcgcagccg
      181 ggaggtgtgg ttcgctgatg ggggtttcct ggcgacgccg gtgcattggc gcgatgggtt
      241 gatgccgggg acggagatcg ctggtccggc gatcatcgag gcgatggatt cgaccaccgt
      301 gatcccgcca cactggcagg cgcgtatcga cggacaggga tacatgcgga tttcgaccgg
      361 gagggttgcg tgatggctcg tctgaagact gatcccgccc ggttcgaggt ggtgaagaac
      421 gcactcacct gcgcggcaga ggagatgaag atcgtcctgg cgaaaaccgc atattcgccg
      481 ctgctgaagg tggcgggcga ttattcgtgc gggattttcg atattcgcgg gaatatggtg
      541 gcgcaggggc ctgacctgcc gatccatctc gggtcgatgc cggatgcggt gcgggcggtg
      601 gtggtggcat ttcccgatgt cgcgccgggc gatgtgttca tccacaacga tccgtaccag
      661 gggggctcgc atctgccgga cgtgaacgtg gtggcgccgg cgtttcatga ggggcgactg
      721 ctggggtttg gctgtgtgcg tgcgcattgg ccggatatcg gcagcgccac accaggcagc
      781 tatggcgcgg tgaccgagat ctacggcgag gggctgcggc tgccgccgat acgactgtat
      841 cgcgacggca aaccggatcc tgccatcgag gcgatcatct tcgccaatgt gcgtacgccg
      901 acggagcggc tgggcgatct gcgcgcgcag gtatcggcca actggcgcgc ggcggcgcgt
      961 ctgcaggcgc tggcggcgaa atacggcacc gatgcgctgc tggagatcat gcaggaggtg
     1021 caggactact cggagaccat gatgcgggcg gctttgcgcg cgctgccgga tggcgaggct
     1081 gaattcaccg atgtgttcga cggtgacggg gtgatcgcgc cgggcgaaac cgacgacgag
     1141 accttcaccg tgaagctgcg gatcaccaag cggggcgata cgatcaccgc ggacttcgca
     1201 gggtctgatc cgcaggtggc ggggccgatg aatgcgccgc tgacggtgac ggcttcgggt
     1261 gtgttctgcg cgttgaagat gatcgctgat ccgaagagtt tgataccgcc gaattcgggg
     1321 tgctggcggc cggtgacggt gacggcgcca cccgggtcgg tggtgaatgc acagcttccg
     1381 gcaccggtgg tctatgccaa tcatgagatg tcgcaccgcg tggcggacat ggtgatggcg
     1441 gcgatgttcg ccttcacgcc gcgcacggtg atggcgggct cgcaagggac gtcggcagtg
     1501 atgacgttcg gcgggacgga ttaccggacg gatgagcgct acgtgagcta tgagtcggtg
     1561 aagggcgggt ttggcgcgcg gccggtgaag gatggcatca atgctgtcgc gtccaccgtg
     1621 tccaacatga tgaacacgcc gatcgagatc gtggagatga gctttccgct gcgcgtggag
     1681 gaatacgcgc tggtgcagga cagcggcggt gccggtacgt ggcgcggcgg gcttggcgtg
     1741 cgccgcgtgt ggcgcgtgct ggagcggcag gcgcatgcgg cggtttgttg tgagcgcacg
     1801 gtgacgccgc cgttcgggtt ggccggcggg caggcgggcg ccgcggcgaa gctggaactg
     1861 atcccgccgc acggcaatgc gcgcaggctc aacagcaagg gcgggttcat tgcaccggcg
     1921 gattccctgg tggtgatcga ggcgccagga tcgggcggtt atgggccgcc ggcggaacgc
     1981 gaccgtgcga gtctttccga agacctgaag gacgggtacg ttagtgccgc agcggctcgg
     2041 cgcgactacg gcgtcgtggc ggccggcgaa tagggagaac aggaaatgtc aggcgcacaa
     2101 ccgacgatcg ctcagaaaag cccgtaccgg atggaagtgg aggccggcaa gtcgtactgg
     2161 tggtgcgcct gcggcaagag cgccagccag ccgttctgcg atggctcgca tcgcggcagc
     2221 ggattcacgc cgatcgaata caaggccacg gaagacggca ccattccgtt ctgcggctgc
     2281 aagatgagca acggcaagcc tcgctgcgac ggtacgcatc ggagcctgtg acgaccctcc
     2341 accggaggaa cgacaatgaa gatcgatgac gtttcgctga ccatcttcac atgggatggc
     2401 ataccggcga ccaattacca cacgggaacc gcgacggccg gcaacagcaa tctcgggttg
     2461 ctgcgtatcc acacggatga ggggctggag gggcacgcat tcctcggttc ggcgaccaat
     2521 ccggcgtcga tggatgggcc gcagctgatc cgctcgctga agccgatgct gatggggcag
     2581 aacccgctcg aacgcgaacg gctgcacggc gcgatgcggc tgatctcgcg cgccgtgtcg
     2641 tatcggacca tcggtgccgt ggatacggca ctgtgggatc tgggcggcaa gatcgcgaag
     2701 ctgccggtgc atgcgctgat ggggacgacc cggacctcga tcatggggta tgcgagttcg
     2761 caggttctgc cggatatcgc ggcctatgtg gaccaggcgc agacgttcaa ggcgaatggc
     2821 tggcgcgcgt acaagatcca tccaccgcgc gatccggagc aggacatcaa ggtgtgccag
     2881 gccgtgcgca aggcggtggg ggatgatttc cgggtgatgc tcgattccac ctggagctac
     2941 aattatgtgg acgcgctgcg cgtggggcgg gcgatcgagg aacttggatt ctattggtac
     3001 gaggatccga tgtccgacga agacatctac aactatgtga agctgcgcga gaagctggat
     3061 attccgatca tggcgacgga gtttccggcg ggcgggctgg agacgtatcc gatctggctt
     3121 accgagcgtg ccaccgacta tctgcgcggc gatatcccga acaagggcgg gctgaccacg
     3181 atgctgaaga ccgcgcatct ggcggaggcg ttcagtctga aatacgagat ccaccactcg
     3241 gggaattcgc tgaacaatct cgcgaacctg catctgtgca tggcgatccg gaacacgacg
     3301 atgttcgagg tgctgctgcc ggatggggcg cacaagtatg ggatggcgaa ggagctggaa
     3361 atcgatcgcg atgggatgct gcatgcgccg acgggaccgg ggctggcggg cgagatcgac
     3421 ttcgagctga tcaagcgcaa gacggaagct gtgctgtcgt gaattatggc acttgtgcgc
     3481 ctggcgggag atggccgggt caagcccggc catgacgtga gtcgcagccg acaagttcgc
     3541 ccagggagat cggcttcagc ggcgggcgga tgcggtaata gccgacttcg cctggtgcca
     3601 caccgcgttc tgcggcgatc agttcggtta ccaccggccc gcatagacgg ccttggcacg
     3661 cgcccatgcc cgcgcgcagg aaggccttcg cctggttggg gccgagacag ccttgccgca
     3721 ctacggcgcg tactgcaccg gcggcgatct cttcgcagcg gcacaccgtg acgtcgtctg
     3781 ccggctgaag gatctcgtcg cgcggcgcat agagcacgtc gaggaaacgc cggatcgcca
     3841 gatgcgcgtg cctcgcgcgc tggtcgttgg cagccagtga gtcgcgctgc gtaacgttga
     3901 tgcggcccag gtggcgcagt gcttcgtacg cagccaaacg gccggcatgt tccgcggcac
     3961 gcgcgcctcc gatgccgccg gcgtcaccag cgatcagcac accgtcgatc gagctgttgc
     4021 cccaggcatc gagccgcggc gcgaagcagc actggacgct gtcccatacg aagtcgcagc
     4081 caatcgaacg cggcagttgc tgggctggta tcacgccctc atgcagggcg acgagacgcg
     4141 tggcgaggcg atgctgcctg cctccgctac ggaacgcgat gccggtgacg gaagcggttc
     4201 cgtctatacg aatgtcagtg acgtgccgat accgtggcac gccactgcga cgcagagcgg
     4261 ccagcatggc aaggcccttg gcgagatagg cacgaccctc gcgggtggca gctgccttgg
     4321 gcagaaagcg cagtgctcgc tgctgatttg cacgtgctgt cgtgtcgagc accgcggccg
     4381 gtcgggcgcc tgtggcgagg cattgctgta ccagcaggta cagcaatggt cccgagccag
     4441 cgatgacgaa tggttcgtct gggaccagcg cggccgattt cagcatgatc tgcagcgcgc
     4501 cggccgtcat cacgccgggc agagtccagc cggggagggg gacagggcgt tccatcgcgc
     4561 cggtggccac gatgatcgta tcgccaacaa cctgctgcga acgaccgccg tggctgaacc
     4621 acacttcgcg ctgtggcgtc acctgccaga cctgcgcgcc tggctgatat gttgcgccgc
     4681 ttgcacggaa gcgtgcggtg agttgggcgc cgtactggta gtctgcgccg agggccaccg
     4741 ccagtgccgg cttcgcgacg gcgcattcga tgttgcgata gatctggccg cccggcgcgg
     4801 gctgttcgtc cagcagcagc acaggcacct gctgttccgc cagcagtgtt gcggcaacca
     4861 gccccgctgg ccccgcgcca atcaccacca cctgggtcac aacacgcctc gcgcgccacg
     4921 ctggcgccgt acctgcatgc cctcagtggc gggggtcagg caggcctggc ggttgccgac
     4981 accgtcgatc tccatcaggc actcgaagca gacgcccatc atgcaatagg gcgcacgggg
     5041 cgaatttgac acggcagtca tgcgcaatgt cgtttcgccg gcagcgatga gcgctgcggc
     5101 caccgtgtca cctgggcgcg cggtgatggc gcggccttcg tagtcgaaac ggatggcctc
     5161 ggcgggcggt tcaagccttc gccacatcga accgcctgct gttgaaggca tcgaagcgtt
     5221 cggggagttc gcccgccatg atcgctggtg ccagagcgag cgcgtgggcg ccggccagcg
     5281 tgacaccgct gtggcaggtc gcggcgaatg caccgggata gcgctgcgat tgctcgtaga
     5341 tggggaagcc gtcgggcgac atcacgcgca gtgctgccca ggtgcgcacg atcgtggcgt
     5401 ccttcagtgc cgggaacacc gcgaggttgc ggttggcgat gtcctgcagc accggcacgc
     5461 caatcgacgt atcgaatccg gcgtcttcct gcgagtcgcc tagcatcacc gtaccctcga
     5521 tcgtctggcg cagtgtgtgg caggtgtagt cgaggaatgg ccgcacgcgc tcggtgacga
     5581 tgatctggcc gcgcgacggc accacaggca tactgagacc gaccatcggg gcgagttcgc
     5641 ggctgccgag accggctgcg aggatcactt tcgccgcggt gagatgttcg gcgccgatgc
     5701 ggacggtgaa gttgttgggc gccgcatcga tcgcatcgac ggtacggcct gggagatatt
     5761 cgccgccgag cttcagcagc gcggcatgca gcgcacgcag cagatagagc gagtgcgcct
     5821 cgccatcgat cggacaatac gacgcgccga caacgcgatc acccaggcca ggcaccatcg
     5881 cctgcacctc ggcacggtcg atcatgcgcg tgccgatatc gccgctttcg ttgtgcatgc
     5941 ggtggagcag ggccgcgcgg tcctcgaact ccttgtcact gaggcagaag gccaggccgc
     6001 cgttctgctt gagggcgacg tcgatgccgg tggattcggc catcgcactc gacagttgcg
     6061 gccagagttc ggccgaggtg cgggtccagt gcgcgtaggc gggcaacccg gcgcctttgc
     6121 tctgcaccca caccaggccg aagttgccgc gtgcggcgcg gaaggcgata tcgccctgat
     6181 cgatcagtgc gaccgacgca ccttcgcgcg cggcacccca ggcgatggct ccgccaacca
     6241 ggccgccgcc gatgacgata agatcgtagg ccat
//
LOCUS       DATGEM010000160        14876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1354766, whole genome shotgun sequence.
ACCESSION   DATGEM010000160 DATGEM010000000
VERSION     DATGEM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354766"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VKQ27_04960"
     CDS             <1..447
                     /locus_tag="VKQ27_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88314.1"
                     /translation="IFGGNGITTFGVPDLRGRIPLPFDSTGARITAALSGLSGNVLGA
                     ALDQQVVGLTPTQIPNITSNVNGVSAAKNYLTSPVTGYGITAFSANAGSQVWQAFSSG
                     ASIIVESTVVITGTATSNNTSGGSPGAGHNNVQPSLMTGIAVIRAG"
     gene            458..2506
                     /locus_tag="VKQ27_04965"
     CDS             458..2506
                     /locus_tag="VKQ27_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88315.1"
                     /translation="MPFGAVKLVPGVNVEKTPTLNEAGISASQLIRYQDGLVQKYGGW
                     QAFYPLAVGGVPRDLHAWEDLNNVSRLAIGTTTQLVTIDDTQTVKAITPQTLITNFAP
                     NFSTVNGSPTVNVVDAGISNVTAFDSVFFNTPVTIGGIILSGLYPISAAVGTHSYSII
                     AATPATATVNNAGAVPVFTSTINSANVSVALANHGLGAAPDNSIVFPIPTVVNGITVS
                     GLYAAASITDANNFVIQTNAQATASGAVSMNGGQAQIEYYIALGPAAGGVGYGLGGYG
                     LGGYGTGVPNNVQTGTPIAAADWTTDNWGQILLACPEGGGIYYWDPTGGFDTASLISS
                     GPIFNAGMFVSTSAQIVIAYGSTISEQIGVVQDPMLVQWSDSGNFFDWTPTDTNLARN
                     FRIPIGSRIVTGMAVSNQNLIWTDLDLWIMNFIGFPNVYGFNKIGAGAGAASSHAAQQ
                     LRGGVYWMGASNFYRYAGSGVEVIPCPVWDAVFQNINTAFLQNVRAMPNTAFNEVGWL
                     YPSAASVSGENDSYVKMNITEPNQPWDYGLLPRSAWIDQNVFGPPIGAIPGGVIYQHE
                     TSPDAGGQPLSWSYTTGYFKIAEGQEYAFVDQWRPDFKFGTFSGSPAAQIQMTFNVVN
                     FPGDTPTQYGPYTVTQSTEFLSTRFRGGLVSITVSGSDLGSFSRLGYVRYRYSSTGRR
                     "
     gene            2506..2724
                     /locus_tag="VKQ27_04970"
     CDS             2506..2724
                     /locus_tag="VKQ27_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88316.1"
                     /translation="MAAADLDTINSTQTAGVQYLGQIVAALRAAFGFLGGTAPSATGG
                     AATLPANPVGFVTMTLPSGASVKVPYYN"
     gene            2732..7783
                     /locus_tag="VKQ27_04975"
     CDS             2732..7783
                     /locus_tag="VKQ27_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88317.1"
                     /translation="MPLRKGSSRSTVSDNIRELHTGPTYAHTESKFGKRRADKQAVAI
                     ALNVARRARAEGGAVDDGPYRGPNLGPLQGGPDLPDVGGPVGPQFSRGFDWANRAADI
                     GSKVARGAGEGLIRDISTPGAMMKANPYPPGSEEADWYDGQRDKAATDWAPGMALNTM
                     GASSVGMPARAAGEVLAGAGPAGRSPRPALDMAPEARAARAQEQGYNLDLYHGTSAPE
                     FDTFKRRQNDLGIHLGTAEQANERLSYMADKRSRPQGAEPGEGDRIVPVKAAIRNPLR
                     LSDLGTWNSDNLYYGLQQAGFPEAELRRAMASAGNESGKVAALRDLIERRGHDGVVYK
                     NTGEVAGSEPFRAKVAEARAKIDEAFGRGKNGFTLEDQQHPAYQEWSKAQKDYETHRE
                     QNGQDSYIAFRPNQLRSPTAAFDPANRSKGMLLGSGATDRRAAGISALADVDRVLEKE
                     RASRGAEPVPAEPAAAPESGQVGPGGGGAPGGGSLPEAQAAAERRAGGRPTLTGQQGP
                     LKIGEDYFVPGPIGKIHDVAEQYMREAHPDRPYTPPTEYHPLDPEHSKAIAQAYEEMQ
                     HTPNDPATRASYDALIKETADQYRAIKKTGLKIEPIPAGMPDPYAANPRLAAKDVADN
                     NHLWFYPTEQGFGTVNKISDNPMLRDTGEKIGDHPMLANDMFRVVHDYFGHLKEGHGF
                     RAAGEDNAWRTHAQMYSDIARPAMTAETRGQNSWVNYGPHGEANRKASAADTVYADQK
                     VGLMPEWTMRDRNSPAPIMVYHGTPHTFDRFDISKIGSGEGNQAYGHGLYFAGHEPVS
                     EWYRHQLATRQDPLLKKYGLEEVGHVIGSHLSDAGGDAAKLAAQYAQNRDQLMRDGPV
                     QQPAYQGWHDKATSNMIDEYGRRIEYLNDPERSKGHLYQAGIHVNPEHLLDYQAPFNQ
                     QTGHVQERVDAPMSESVARQREAISRELEKGGRGVVGGRFFRPFTTSQRRDLEGRLML
                     LEQAGHSVFPGSEIYKRMGLPAKDANEAGVKASQRLRDLGVPGLHYPDAGSRAPGQKG
                     SRNYVMFGDEPIKILRRYEQGGAVDRAMNVARRMRRAEGGGVDDSDVIATIPVMKQLG
                     LKGSDITAGDVNNAMNIGMSAGPGTIGAKGMPPARMVDDTGAAMQPARGAKRYAEPEY
                     TESGSHIINKGVENPPDPSIQTVADPYRMMFPGVYRNPRTIAEEAAARVGEEDPAMKR
                     LFGVTRGDLREMAQGRVGNEEPRLHTVGNPRGAVSAQNIQTPQNTQRLVDILGEAGQH
                     EGLRTADAWYIMDPVYRRMEELHGPEAAERYRKFNTLTGMASPGSDVMTEIQRGTGAH
                     WLNEQGRFGDFQKYAGVPEGMRSSMKYPADMRYISGHPYHRTSQGDPMAKYLEAGSIQ
                     SKAPKVPLYVHASGVPETGFQTSGPVGDAHFSRGVGLSDTRKGPSDVQGSFSTSEYQT
                     LQPWWQHDVAGKVGLESVPAQARLWTALGPQTGVESALGAPKLELLSKQIMETARRLR
                     ITPENARDLVLSGKAGAGLVAGGVMGPAVYQAMQQPEQRAAGGAVSNALAIARSLKRA
                     SGGKVHVGPIMGDTDGRADEVPMAVPDGAYVLTADHVSSLGEGNTLSGFKKLNKMFPE
                     SAKARAERKPVKRAQGGAVPIYAADGEYVICPSDIINRWGDLDEGHRYLDAWQTSSRK
                     EHVNTLRNLAPPAQD"
     gene            7787..8056
                     /locus_tag="VKQ27_04980"
     CDS             7787..8056
                     /locus_tag="VKQ27_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88318.1"
                     /translation="MDNKSEVVFITDLEVSGFLNGVINMAFSTTQYVPEFRPAERDGE
                     TFMELAVSPAPVITANLRFDLRVAQQIRDRLNQIIADNTKPKVTN"
     gene            8056..8532
                     /locus_tag="VKQ27_04985"
     CDS             8056..8532
                     /locus_tag="VKQ27_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88319.1"
                     /translation="MLVTLPVRKARPSDRDTILKICVQNHEENGQFSLDAKKVEAMVD
                     RAFSNQGAVIGVVGRDRIEGIIMMLIAQFWYTQDWCLEEIMNFVLPDYRRTTHAKDMI
                     TFAKRCSEEIGIPLVIGVVSNERTKAKIELYRRQLGDPVGGYFIHRPGLASPGATV"
     gene            8605..11631
                     /locus_tag="VKQ27_04990"
     CDS             8605..11631
                     /locus_tag="VKQ27_04990"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLY88320.1"
                     /translation="MGKGSQTTSTQSTSSADPQAAAAYRDLLTRASGVASTPYQAYTG
                     DLTAPVNAQQQAGIGNINANANFASPYISQAAGLAGASAAPITSADIQQYLNPYTQNV
                     VDATTQQMQHDNAQAMAGLQGNQIAQGALGGNATGVAKGILAGQQNRTDASTVAGLYS
                     QGYGQALGAAQQQQQTGLAGANALANYGISGQNAALAGANAQIGAGSLEQQTQQANLN
                     ALYNQYQMAQAYPYQQTQWLAGIDTGVGSNLGGTSNGQTTGPAPNQTAQYLGAGISAA
                     GLFLSDREAKEDIEHIGHTNDGQKIYRYRYKGSPHYHIGLIAQEAEKRDPENVSRGLD
                     GMRYLDIKGATDDAVSRASGGGVGGTPWSGVQGWIPQMNISAGSGAPHASAPAAPSTQ
                     APSVDYSKIASGISGFKPKLDWGGLGQAGLGNTSGEAWGGGSFMGGDAYGGSSANPLP
                     GLDASDYGEGFARGGGVAGYADGGSPDLQSWLDDDGYNPIDAKVGPGTFADRFSPSQD
                     APLGEMTRGQGIALAGKNGTVARNMPDDVINPDNPVRMPDQAAVEAWRKSPGMGQRGP
                     AAAPVAADDEEEEAPAAATPTSGRARPGLAGASLPGSNFPTPPSAPPIARPSDGSEPG
                     FGLGLLSRNAQTGLLTAGLSMLASRSPFLGNAVGEGGLAGLSAYGAAQQHDQQAAAEA
                     EKLRREAQNSEFTNKLALVKETEAERHNRATEENASDKAPSGYQRLADGTLGFIKGGP
                     HDPEQITAETAARTKKGTELDDDTVDAIAQRVAQGDTRAVIGLGRNPAGIAQIQKRVA
                     EIFKEQGLDHEAGAKQILSNIADQAGRMTAERTQAGIASKLAVYGRNVDNAIGVATKA
                     SEDVSRTGFVPVNKAINSWRTQTGDPKTVALGQALYTLINEYARAIGGGHGTVHDKEE
                     AEQKLNQAYSHQQLVAIMNVMRQEIDMTKKSLPEARQEMHDLYARPSQGPGATPAAGS
                     PAGGGAFTPPPGAIPRTFNGKIYYYDPNTKQPYPGQ"
     gene            11631..14771
                     /locus_tag="VKQ27_04995"
     CDS             11631..14771
                     /locus_tag="VKQ27_04995"
                     /inference="COORDINATES: protein motif:HMM:NF037921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88321.1"
                     /translation="METVRKPAAGYSTQLSPLDEMAYRQWVADHGVPTNPDATAPQDY
                     DMRGFYQGLQQQNPRAQSAIDPNDSKLHYPDFWKTPLHQTLSNESQWAPPNAPSWTDD
                     DKLVQPNGRVVFDDRNQPSDAERLLIAPKKRGGSVSLTAHVNEQIRYVNHNPTDGQKK
                     AGNYQKGHIRLHGLDISVENRKGSWRSGMSLDGKKWRSKLPYHYGYIKRTTGADGDHV
                     DVCIGPHLASPHVFVIDQHNLHGGRGFDEHKVFVGFASEAQARKAYIAAFSDGKGSKR
                     IGHIAHMTIDGFKDWLRNGNTLKAVKRAEGGRVGYAGGGAPPFEATSDVPPFEATQEL
                     PSDAGKMDAAWRGARAGATFNFGDEISGARANAPKVPGTNWNIPDFVGPIPARTIAGA
                     ARTGYGYLTGNKDITDPYEKARDEERKADEAAKVNHPYISAGAELAGAIPSMAALPEL
                     GAARGLAPAASGIARFGARALDSAATAGGYGMLSGAGEGDSVGDRAVKAATGLVSGIV
                     GGAVGQGVGEGMGAIASHYGTPIVQTVRGWMDPNGEAARRLASALRADQEMITAGTAQ
                     GMTPQQWAAARARGEPVTLADLGSGRTQALLRSAANTSPEGRAQLEQVLENRFLAQNE
                     RVADTFRNALPQGRANADKSADQIVAEYDRGRVPAYRAAYAQGDRPLMSPAMERMMSS
                     DTFVNAMRKAISSGKDRDVAQGLGGFNPMVNVTPDGRVVFNKGAKGVPTYPNLQYWDQ
                     VKRELDAGATMAKRSGDTANVSGDLARMLRNELDQQVPSYAHARGIAEQYFGESNALE
                     AGQKLAGKKMDPVQVRKILAQMKPDERALFQEGYASDMANRVIGNIGKTTNLVKGRGI
                     LSSPNEEAMARAILGPGGYAQVEARMHLERIMDGARQAMGNSTTARQLIEAGLAGGAA
                     GYAMTGDPWGAVQGAAGLATARGAAGKLLSDQIRVGAKHLIGKVDASTARRVAELLTS
                     NDPRQLRAGYQMAARSNRIMQGLRNIAARVGVVGQTAASRPAGQAIASIPYRGANLGP
                     LQGPSNANAGDEQQRP"
BASE COUNT         2882 a         4839 c         4897 g         2258 t
ORIGIN      
        1 atctttggtg gcaacggcat cacgaccttc ggcgtgcctg atttgcgcgg tcgcatcccg
       61 ctcccgttcg actccaccgg cgcccgcatc acggcggccc tctccggcct tagcggcaac
      121 gtcctcggcg ctgctctcga ccagcaggtc gtcggtctca cgccgacgca gatcccgaac
      181 atcacatcca acgtcaacgg cgtctcggcc gccaaaaact atcttacgtc gcctgtcacc
      241 ggctacggca tcacagcatt tagcgcgaat gcgggctcgc aggtgtggca ggcattctcg
      301 tctggcgcct ccatcatcgt ggagtccacc gtcgtgatta cgggaacagc cacctccaac
      361 aatacttcgg gcggctctcc aggcgctggt cacaacaacg tgcaaccgtc cctcatgaca
      421 ggcatcgccg tcatcagggc ggggtgaagg gggaagcatg cccttcggcg cggtgaagtt
      481 ggtccctggc gtcaacgtcg agaagacgcc gaccttgaac gaggccggca tctcggcgag
      541 ccagctgatc cgctaccagg acggcttggt ccagaagtac ggtgggtggc aggcgttcta
      601 tccgctggcc gttggcggcg tgccgcgcga cctgcatgcc tgggaagacc tcaacaacgt
      661 gagccgtctt gcgatcggca ccaccacgca attggtgacg atcgacgaca cgcaaaccgt
      721 caaggcgatc acgcctcaga cactgatcac caactttgcg ccgaacttct cgacggtgaa
      781 cggctcaccc acggtgaacg tcgtcgatgc tggcatctcg aacgtcaccg cgttcgactc
      841 ggtgttcttc aacaccccgg tcacgatcgg cggcatcatt ctttcggggc tatacccgat
      901 ctctgccgct gtcggcaccc acagctactc catcatcgca gcgactccgg cgacagcgac
      961 cgtgaacaat gccggcgcgg tgccggtctt cacctcgacg atcaatagcg ccaacgtctc
     1021 cgtcgcgctc gccaaccatg gcctgggggc ggcgccggac aactcgatcg tcttcccgat
     1081 cccgacggtt gtgaacggca tcacggtcag cggcctctat gcggcggcct cgatcactga
     1141 cgcgaacaat ttcgtcatcc agaccaacgc gcaggcgacc gcctcgggcg ccgtctccat
     1201 gaatggtggg caggcccaga tcgaatacta catcgcgctg gggcctgcgg ccggcggcgt
     1261 gggctacggt ctcggcggct acggtcttgg tggctacggc accggcgttc cgaacaacgt
     1321 gcagaccggc acgccgatcg cagcggcgga ctggaccacg gacaactggg gccagatcct
     1381 gctggcgtgc cccgagggcg gcgggatcta ctactgggat ccgaccggag gcttcgacac
     1441 tgcctcgctg atctcctcgg gcccgatctt caacgccggc atgtttgtgt cgacgtcggc
     1501 gcagatcgtg atcgcctacg gctccaccat cagcgagcag atcggcgtcg tgcaggatcc
     1561 gatgctggtg cagtggagcg acagcggcaa cttctttgac tggacgccca ccgacaccaa
     1621 ccttgcgcgc aacttccgca tcccgatcgg ctcacgcatc gtgaccggca tggccgtgtc
     1681 gaaccagaac ctgatctgga ccgacctcga cctctggatc atgaacttca tcggcttccc
     1741 gaacgtctac ggcttcaaca agatcggggc cggcgccggt gctgcgtcgt cgcatgcggc
     1801 acagcagctg cgcggtggtg tctactggat gggagcctcg aacttctatc gctacgccgg
     1861 cagcggcgtc gaggtgatcc cctgcccggt gtgggacgcg gtctttcaga acatcaacac
     1921 tgccttcctg cagaacgtgc gggcgatgcc gaacaccgcg ttcaacgagg tggggtggct
     1981 ctatccgtcg gcggcatccg tcagcggcga gaacgacagc tacgtcaaga tgaacatcac
     2041 ggagccgaac cagccgtggg actacggcct gctgccgcga tcggcgtgga tcgaccagaa
     2101 cgtcttcggt ccgccgatcg gcgcgatccc aggcggcgtg atctaccagc acgagaccag
     2161 cccggatgcc ggcggccagc cgctgtcgtg gtcctacacg accggctact tcaagattgc
     2221 cgagggccaa gaatatgctt tcgttgacca gtggcggccg gacttcaaat tcgggacgtt
     2281 ctcgggctcg ccggccgcgc agatccagat gacgttcaac gtggtgaact tccctggcga
     2341 cacgccgacc cagtacggtc cctacaccgt gacgcagtcg accgagttct tgtcgacccg
     2401 cttccgtggc ggcctcgtca gcatcacggt ctccggaagc gaccttggta gtttctctcg
     2461 attggggtat gtgaggtacc gctactcgtc aacagggaga cggtgatggc tgccgcagac
     2521 ctcgacacaa tcaactccac ccagactgcc ggcgtgcagt atctcggcca gatcgtcgcg
     2581 gcattgcgcg cggccttcgg cttcctcggt gggacggcgc cgtcggccac cggaggcgct
     2641 gcgaccttgc cggccaaccc ggttggcttt gtcacgatga cgttgcccag cggcgcatca
     2701 gtcaaggtgc cgtactacaa ctgagggtgc catgccgctc cgcaagggat catcccggtc
     2761 gaccgtcagc gataatattc gcgagctgca cacgggtccg acctacgcgc acaccgagag
     2821 caagttcggc aagcgccgcg ctgacaagca ggctgtcgca atcgccctta acgtcgcgcg
     2881 tcgggcgcgc gccgagggtg gcgccgtgga tgatggcccc tatcgcgggc cgaacctcgg
     2941 tccactgcag ggcgggccgg acctcccgga tgtcggcggg ccggtgggtc cgcaattctc
     3001 gcgtggcttc gactgggcga atcgcgcggc cgacatcggc tccaaggtgg cccgtggtgc
     3061 cggagaggga ctgatccgcg acatctcgac ccctggcgcg atgatgaagg ccaaccctta
     3121 cccgccgggc tccgaggagg ccgactggta cgacggccag cgcgacaagg cggccacgga
     3181 ctgggcgccc ggcatggccc tcaacaccat gggcgcgtcg tctgtaggaa tgcctgcgcg
     3241 ggccgctggt gaggttttgg cgggcgctgg tcctgcgggc cggtcaccgc gtccagcgct
     3301 ggatatggct ccggaggctc gcgcagcacg cgcccaggag cagggttaca acctcgacct
     3361 ctaccacggg acgtctgcgc cggagtttga tactttcaag cggcgccaaa acgatctggg
     3421 tattcatctc ggcaccgccg agcaggctaa cgagcgtcta tcctacatgg cggacaagcg
     3481 gtctcgtccg cagggcgccg agcctggaga gggcgaccgg attgtccccg tcaaggcggc
     3541 catcagaaac ccgcttcggt tgagtgatct cggcacatgg aatagcgaca atctgtatta
     3601 cgggcttcag caggccggat ttccagaagc tgaattgcgc cgtgcgatgg catcggcagg
     3661 caatgagagt ggcaaagtcg ccgcactgcg cgatctgatc gagcgtcgcg gccacgacgg
     3721 tgttgtctac aagaacacgg gagaggttgc cggttccgag ccattcagag ccaaggtcgc
     3781 ggaggcgaga gccaagatcg atgaggcgtt tggccgaggt aaaaatggtt ttacgctaga
     3841 ggatcagcag catccagcct accaggagtg gtccaaggcg cagaaagatt acgaaaccca
     3901 ccgggagcag aacggccagg acagctatat cgcctttcgg ccaaatcagt tgcggtctcc
     3961 gacggctgcc ttcgaccctg caaatcgatc caaggggatg cttcttggct ccggcgcaac
     4021 cgacaggcgc gccgctggca tttccgcgct cgctgatgta gaccgtgtcc tcgaaaagga
     4081 aagagcatcc cgtggcgcag aacccgtacc cgcagaacca gcagcagccc ccgagtccgg
     4141 acaagtggga cccggaggag gaggggcgcc tggaggcgga agcctaccag aagcacaggc
     4201 agcggcagag cggcgagcag ggggccgccc gaccctcacc ggccagcaag gcccgctaaa
     4261 gatcggcgag gactacttcg tccccggccc gattggcaag atccacgacg tcgctgagca
     4321 gtacatgcgg gaggcccacc cggaccgccc gtatacgccg ccgaccgagt atcatcccct
     4381 cgacccagag cactcaaagg cgattgctca agcctacgag gagatgcagc acacgccgaa
     4441 cgatcccgcg accagggcat cgtatgatgc gctgatcaag gaaacggctg accagtatcg
     4501 ggcgatcaag aagaccggtc tgaagatcga gccgatcccg gccggcatgc ctgatcccta
     4561 tgctgccaac ccacgcctcg cggcgaagga cgtcgccgac aacaatcacc tgtggttcta
     4621 tccgacggag cagggattcg gtacggtcaa caagatctcc gacaacccga tgctgcgcga
     4681 caccggcgag aagatcggcg accacccgat gctcgccaac gatatgttcc gcgtcgttca
     4741 cgactatttc ggtcacctga aggaaggtca cggcttccgc gccgccggtg aggacaacgc
     4801 ttggcgcacg cacgcgcaga tgtattcgga catcgcgcgg ccggcgatga ccgccgagac
     4861 gcgcgggcag aactcgtggg tcaactacgg tccccacggc gaagcgaacc ggaaggcctc
     4921 ggcagctgac acggtgtacg ccgaccagaa ggtcggactg atgccggagt ggacgatgcg
     4981 cgaccggaat agcccggcgc cgattatggt ctaccacggc acgccgcaca cgttcgatcg
     5041 cttcgacatc tccaagatcg gctctggcga gggcaatcag gcgtacggcc acggcctcta
     5101 cttcgccgga catgagccgg tcagcgagtg gtaccggcat cagctcgcga cgcgccagga
     5161 tccgctgctg aaaaagtacg gtttggagga ggttggccac gtcatcggct cgcacctgtc
     5221 ggacgccggt ggcgacgcag cgaagttggc cgcccagtat gcccagaacc gcgaccagtt
     5281 gatgcgcgat gggccggtgc agcagccggc ctatcagggg tggcacgaca aggccacgtc
     5341 gaacatgatc gacgagtacg gtcgtcgcat cgaatatctc aacgacccgg agcggtcgaa
     5401 ggggcatctg tatcaggccg gcatccacgt caacccggag catctgctcg actatcaggc
     5461 cccgttcaat cagcagactg gccacgtaca ggagcgtgtc gacgctccca tgtccgagag
     5521 tgttgcccgg cagcgagagg cgatctcgcg cgagttggag aagggtggga ggggtgttgt
     5581 cggagggcgc ttcttccggc cgtttaccac gtcacagcgg cgcgaccttg aaggccgcct
     5641 gatgctgctg gaacaggccg gtcattcggt gttcccaggc agcgaaatct ataagcgcat
     5701 ggggcttcct gccaaggacg ccaacgaggc cggcgtcaag gcatcgcagc gcctgcgaga
     5761 tcttggcgtc cccggcctgc actaccctga cgccggctcg cgcgcaccgg gccagaaagg
     5821 atcacgcaac tacgtgatgt tcggtgacga gccgatcaag atcctgcgtc gctacgagca
     5881 aggtggtgcc gtcgatcgag cgatgaacgt cgcgcgccgt atgcgccgtg ccgaaggtgg
     5941 tggcgtcgat gattcggatg tcatcgcgac gatccccgtg atgaaacagc tcggcctcaa
     6001 gggttccgac atcacggccg gcgacgtcaa caacgcgatg aacatcggca tgtcggccgg
     6061 gcccggcacc atcggcgcca aaggcatgcc accggcgcgc atggtcgacg acaccggcgc
     6121 tgcgatgcag cctgcacgag gtgccaagcg ctacgccgag cccgagtaca ccgagagcgg
     6181 cagccacatc attaacaagg gcgtcgagaa cccgccggat cccagcatcc agaccgtggc
     6241 cgatccctac cggatgatgt tcccaggcgt ctaccgtaac ccccggacga tcgccgagga
     6301 ggccgcagcg cgcgtcggtg aagaagaccc ggcgatgaag cggctgttcg gtgtcacccg
     6361 tggcgacttg cgcgagatgg cgcaggggcg cgtcggcaac gaggagccga ggctgcacac
     6421 tgtcggcaac ccgcgcggcg cggtgtcggc gcagaacatc cagacgccgc agaacaccca
     6481 gcggctggtc gacatcctgg gtgaggcggg ccagcacgag ggcctgcgca ccgccgacgc
     6541 ttggtacatc atggatccgg tctatcgccg gatggaggag ctgcacgggc cggaggcggc
     6601 cgagcgctac cgcaagttca acacgctgac cggcatggct tccccaggat ccgacgtgat
     6661 gaccgagatc cagcgcggca ccggcgcaca ctggctcaac gagcagggtc gcttcggcga
     6721 tttccagaag tatgccggcg tgccggaggg catgcggtcg agcatgaagt atccggccga
     6781 catgcgctac atctcgggcc acccctacca ccggacgtcg cagggcgacc cgatggcgaa
     6841 gtacctggag gccggctcga tccagagcaa ggctccgaag gtgccgctgt acgtccatgc
     6901 ttcaggcgtt ccggagaccg gcttccagac gagtggtccg gttggtgacg cgcacttcag
     6961 ccgtggcgtc ggtctctccg atacccgcaa ggggccaagc gacgtgcagg gctcgttctc
     7021 gacgagcgaa taccagacgc tgcagccgtg gtggcagcac gatgttgctg gcaaggtggg
     7081 cctggagagc gtgccggcgc aggctcgcct gtggaccgcg ctgggcccgc agactggtgt
     7141 cgagagcgca ctcggtgctc cgaagctgga gctactgtcg aagcagatca tggagacggc
     7201 gcgccggctg cgaatcaccc ccgagaatgc gcgcgatctg gtgctgtccg gcaaggctgg
     7261 tgccggcctc gtcgctggtg gcgtcatggg tcctgcggtg tatcaggcga tgcagcagcc
     7321 cgagcagcgc gcggccggcg gcgccgtcag caatgcgctg gcgattgcac gcagcctgaa
     7381 gcgtgcatct ggcggcaagg tgcatgtcgg accgatcatg ggcgacaccg acgggcgcgc
     7441 cgacgaggtg ccgatggcag tgccggacgg tgcctacgtg ctcacggcgg accacgtctc
     7501 ctcgctggga gagggcaaca cgctctcggg cttcaagaag ctgaacaaga tgttccccga
     7561 gtccgccaag gcgcgcgcgg agcgcaagcc ggtgaagcgc gcgcagggcg gcgccgttcc
     7621 gatctatgca gccgacgggg aatacgttat ttgtccttcg gacatcatca atcgctgggg
     7681 cgatctcgat gaaggtcacc gctacctcga tgcgtggcag acctcatccc gtaaagagca
     7741 cgtcaacacg ttgcgcaacc tcgcgccccc agcacaggac tgatcgatgg acaataaatc
     7801 cgaagtcgtt ttcatcaccg accttgaggt ttcagggttc ctgaacggcg tcatcaacat
     7861 ggcattctcg acgacgcagt acgttcccga gtttcggcct gcggagaggg acggcgagac
     7921 cttcatggag ttggctgtgt cgccggcacc ggtcatcacc gccaacctgc gcttcgatct
     7981 ccgtgttgcg cagcagatcc gcgatcgtct caaccagatc atcgctgaca acactaagcc
     8041 gaaggtcaca aactgatgct ggtaacgctc ccggtccgca aggcgcgccc atcagatcgc
     8101 gatacgatcc tcaagatctg cgtgcaaaac cacgaggaga acggacagtt ctccctcgac
     8161 gcgaagaagg tcgaggcgat ggtggaccgg gccttctcga accagggcgc cgtcatcggc
     8221 gtcgtcgggc gcgatcggat cgagggcatc atcatgatgc tgatcgcgca gttctggtac
     8281 acccaggact ggtgcctgga ggagatcatg aatttcgtac tgccggacta ccggcgcacc
     8341 acgcacgcga aggacatgat caccttcgcc aagcgctgca gcgaggagat cggcatcccg
     8401 ctggtgatcg gcgtggtgtc gaacgagcgc accaaggcaa agatcgagct gtaccgtcgc
     8461 cagctcggcg atcctgtggg cggctacttc atccaccgac cggggctggc gtcgcccggc
     8521 gcgacggtgt agggatagac agctaatcac ccagcgcgcc gccgggtctt acaagccaat
     8581 cgggccgatt ggtggaaaga gaccttgggt aagggcagcc agactaccag cacgcaatcc
     8641 acatcttcgg ccgatccgca ggcagctgca gcctatcgtg atctgctgac gcgagcgtcg
     8701 ggcgtcgcct ccactcccta ccaagcctac accggagacc tcactgcgcc ggtcaacgcg
     8761 cagcagcagg ccggcatcgg caatatcaac gcgaatgcca acttcgcgtc gccctacatc
     8821 tcgcaggcgg ctggtcttgc cggagcgtcg gccgcgccga tcacgtcggc cgacatccag
     8881 cagtatctca atccgtacac ccagaacgtt gtcgacgcga cgacgcagca gatgcagcac
     8941 gacaacgcgc aggcgatggc ggggctgcag ggcaaccaga tcgcgcaggg cgcgctcggc
     9001 ggcaacgcca ccggcgtcgc gaagggcatc ctggccgggc agcagaaccg caccgatgca
     9061 tcgaccgtcg ccggcctcta cagtcagggc tacgggcagg cgctcggcgc cgcgcagcag
     9121 cagcagcaga cgggtcttgc cggagcgaac gctctcgcca actacggcat ctcgggccag
     9181 aacgcggcgc tcgctggcgc caacgcgcag atcggcgcgg gctcgctgga gcagcagacc
     9241 cagcaggcca acctcaatgc gctctacaat cagtaccaga tggcgcaggc ctacccgtac
     9301 cagcagacgc agtggctggc gggcatcgat accggcgtcg gatcgaatct gggcggcacg
     9361 tcgaacgggc agaccacggg gcccgctccg aaccagacgg cgcagtatct cggcgccggc
     9421 atctcggcgg ctggcctgtt cctgtccgac cgcgaggcga aggaagacat cgagcacatc
     9481 ggtcacacca acgacggcca gaagatctac cggtaccgct acaagggatc gccgcactac
     9541 cacatcggcc tgatcgcgca ggaggccgag aagcgcgacc cggagaacgt ctcgcgcggc
     9601 ctcgacggca tgcgctatct cgacatcaag ggcgcgaccg atgacgcggt gtcgcgcgca
     9661 tccggcggtg gtgttggcgg cacgccgtgg agtggtgtcc agggctggat cccgcagatg
     9721 aacatcagcg ccggctcggg cgcgccgcat gcgtcggctc cggctgcgcc gagcacccag
     9781 gcgccgagcg tcgactacag caagatcgcc tcgggcatca gtggcttcaa gcctaagttg
     9841 gattggggtg gcctcgggca ggccggcctc ggcaacacga gcggcgaagc gtggggcggc
     9901 ggcagcttca tgggtggcga cgcctatggc ggttcgagcg ccaacccgtt gccgggtctc
     9961 gatgcgtcgg actatggcga gggcttcgcg cgcggaggcg gcgtggcagg atacgccgac
    10021 ggcggcagcc cggatctcca gagttggctc gacgacgacg gctacaatcc gatcgatgcg
    10081 aaagtcggtc ccggcacgtt cgccgatcgg ttctcgccgt cccaggacgc gccgctaggc
    10141 gagatgacgc gcggccaggg gatcgccctg gccggcaaga acggaaccgt cgcccgcaac
    10201 atgcctgacg acgtgatcaa cccggacaat ccggtccgga tgccggacca agctgcggtt
    10261 gaggcctgga ggaagtcgcc tgggatgggc cagcgcggcc cagcggcggc ccctgttgcg
    10321 gccgacgatg aggaggagga agcaccagcg gcagccacgc caacctctgg gcgggctcgg
    10381 ccggggcttg caggggcatc tctgccaggg tccaactttc cgaccccgcc gtcggctcct
    10441 cccattgcgc gaccgtccga tggtagcgag cccggctttg gccttgggct actgtcgcgg
    10501 aacgcgcaga ccgggctgct gacggccgga ctgagcatgc tggcctccag gtctccgttc
    10561 ctgggtaatg ccgtgggtga ggggggcctt gccggcctca gtgcctacgg tgctgctcag
    10621 cagcacgatc agcaggctgc ggccgaagcc gagaagctgc ggcgcgaggc tcagaacagc
    10681 gagttcacga acaagttggc gctcgtcaag gagaccgagg ccgaacgcca caaccgcgcc
    10741 accgaggaga atgcctccga caaggcgcct tcgggttacc agcgtctcgc ggatgggacg
    10801 ctcggcttca tcaagggcgg cccgcacgat ccggagcaga tcacggccga gactgccgcg
    10861 cgcaccaaga agggaaccga gttggatgac gacacggtcg acgccatcgc gcagcgggtc
    10921 gcgcagggcg acacccgcgc cgtcatcggc ctcgggcgca acccggccgg catcgcgcag
    10981 atccagaagc gcgtcgctga gatcttcaag gagcagggcc tcgatcacga ggctggcgca
    11041 aagcagatcc tgtcgaatat cgccgatcag gctggtcgca tgacagcgga gcggacgcag
    11101 gccggcatcg cttcgaagtt ggcggtctat ggccgcaacg tcgacaacgc gatcggcgtc
    11161 gccaccaagg cgtcggagga cgtcagccgc accggcttcg tcccggtcaa caaggcgatc
    11221 aattcctggc gcacccagac gggtgatccc aagacggtcg cacttggtca ggcgctctac
    11281 acgctcatca acgagtacgc tcgcgcgatc ggcggcggcc acggcaccgt ccacgacaag
    11341 gaggaggcag agcagaagct caatcaggcc tacagccacc agcagctcgt cgccatcatg
    11401 aacgtgatgc ggcaggagat cgacatgacg aagaagtccc tgcccgaggc gcggcaggag
    11461 atgcacgatc tctacgcgcg gccgagccag gggcctgggg cgacgcctgc cgcaggatcg
    11521 ccagcaggag gcggcgcctt cacgccgccc ccaggcgcca tcccgcgcac gttcaacggc
    11581 aagatctatt actatgaccc gaacaccaag cagccgtatc cggggcagtg atggagaccg
    11641 tccggaaacc agcggccggc tacagcacgc agctcagtcc gctggacgag atggcctatc
    11701 gccagtgggt cgcagatcac ggcgtgccaa ccaatcctga tgcgaccgcg ccgcaggatt
    11761 acgacatgcg tggcttctat cagggcctgc aacagcagaa cccacgtgcg cagtcggcga
    11821 tcgatcccaa cgacagcaag ctgcactacc cggacttctg gaagacgccg ctgcatcaga
    11881 cgctctcgaa cgagagccaa tgggcgccgc cgaacgctcc gtcatggacc gacgacgaca
    11941 agctggtgca acccaacgga agggtggtat tcgacgacag gaatcagcca tcggatgccg
    12001 agaggctgct gatcgcaccg aagaaaaggg gaggatccgt gagcctcacc gcgcacgtca
    12061 atgagcagat ccgctacgtc aaccacaacc cgaccgacgg gcagaagaag gctggcaact
    12121 accagaaggg ccacatccgc ctccacggcc tcgacatctc ggtcgagaac cggaaggggt
    12181 cctggcgctc ggggatgtcg ctcgacggca agaagtggcg atcgaagttg ccgtaccact
    12241 acggctacat caagcgcacc accggagctg acggtgacca cgtcgatgtc tgcatcgggc
    12301 cgcacctcgc aagcccgcat gtcttcgtga tcgatcagca caatctgcac ggtgggcgcg
    12361 gcttcgacga gcacaaggtc ttcgtcggct tcgccagcga ggcccaggcg cgcaaggcct
    12421 acatcgcggc cttcagtgac ggcaagggca gcaagcgcat cggccacatc gcgcacatga
    12481 cgatcgacgg cttcaaggac tggctgcgca acggcaacac gctcaaggca gtgaagcgcg
    12541 ccgaaggcgg gcgcgtcggg tatgctggag gtggagcgcc accgttcgag gcaacgtccg
    12601 atgtgccacc gttcgaagcg acgcaggagt tgccgtcaga tgccggcaag atggacgcag
    12661 cgtggcgcgg cgctcgcgcc ggtgccacct ttaatttcgg tgatgagatc agcggtgccc
    12721 gcgccaacgc gccgaaggtc ccaggcacca actggaacat cccggacttc gtcggtccga
    12781 tcccggcgcg cacgatcgcc ggagccgcgc gcaccggcta cggctacctc acgggcaaca
    12841 aggacatcac cgatccttac gagaaggccc gcgacgagga gcgcaaggcc gacgaggccg
    12901 cgaaggtcaa ccatccatac atttccgctg gggcggaact cgctggagcg attccgtcca
    12961 tggcggcgct gccggaactt ggggcggcca gaggactggc cccggcagcg agcggcatcg
    13021 cgcggttcgg cgctcgtgct ctcgactcgg cggccaccgc tggcgggtac ggcatgctgt
    13081 cgggcgccgg tgagggtgac agcgtcggcg atcgcgccgt gaaggctgct accggtctgg
    13141 tatccggtat tgtcggtggc gccgtcggcc agggcgtcgg cgagggtatg ggagccatcg
    13201 cgagccacta cggcaccccg atcgtgcaga ccgtccgtgg ttggatggat ccgaacggcg
    13261 aggccgcgcg tcgcctcgca tcggcattgc gcgccgacca ggagatgatc accgccggca
    13321 ccgcgcaggg catgacaccg cagcagtggg ccgcagcccg cgcgcgcggt gagcctgtga
    13381 cgctggcaga ccttggctcc ggccgcaccc aggcgctgct gcgctcggcc gccaacacct
    13441 cgccggaggg gcgcgcgcag ctggagcagg ttctcgaaaa ccggttcctg gcccagaacg
    13501 agcgcgtcgc cgacaccttc cgcaatgccc tcccgcaggg gcgggcgaac gcggacaaga
    13561 gcgcggacca gatcgtcgcg gaatacgacc ggggtcgcgt gccggcctac agggcggcct
    13621 acgcgcaggg agaccggcca ctcatgagcc cagcgatgga gcgcatgatg agcagcgaca
    13681 ccttcgtcaa cgcgatgagg aaggcgatct ccagcggcaa ggaccgcgac gtcgcgcagg
    13741 gcctcggcgg cttcaatccg atggtgaacg ttacgcccga tggtcgcgtg gtgttcaaca
    13801 agggagcgaa gggtgtcccg acctacccga acctgcagta ttgggatcag gtgaagcgtg
    13861 agttggacgc cggtgccacc atggcgaagc ggagcggcga cacagcgaat gtctctggcg
    13921 atctggcccg catgctgcgc aacgagctgg accagcaggt cccgtcctac gcgcacgcac
    13981 gcggcatcgc cgagcagtat ttcggcgaga gcaacgctct ggaagcgggg cagaagttgg
    14041 ccggcaagaa gatggacccg gtgcaggtcc ggaagatcct cgcccagatg aagcccgacg
    14101 agcgcgccct gttccaggaa ggctacgcct ccgacatggc gaaccgggtc atcggcaaca
    14161 tcggcaagac cacgaatctc gtgaagggtc gcggcatcct gtcctctccg aacgaggagg
    14221 cgatggcgcg ggctatcctg ggcccaggtg gctacgcgca ggtcgaggcc cgcatgcact
    14281 tggagcgcat catggacggc gcgcggcagg cgatgggaaa ctcaacgact gctcggcagt
    14341 tgatcgaggc aggccttgcc ggcggcgcgg ccggatacgc catgaccgga gatccgtggg
    14401 gtgccgtgca gggtgcggcc ggtctagcca ccgcgcgcgg cgccgcaggg aaacttctca
    14461 gcgatcagat ccgggtcgga gcaaagcacc tcatcggcaa ggtggacgcg agcaccgcgc
    14521 gacgggtggc cgaactcctc acctcgaatg atccgcgcca gctgcgcgcc ggttatcaga
    14581 tggccgctcg cagcaatcgg atcatgcagg gcctgcgcaa catcgcggcg cgggtgggag
    14641 tggttgggca gactgcggcc agccgtcccg ctggtcaggc aatcgcctcg atcccgtatc
    14701 gtggtgcgaa ccttggcccg cttcagggcc catcgaatgc caacgctggc gacgaacagc
    14761 agcgcccctg acggaaacgg tatcagcagc cagcaggccg cgagcacgaa agcaaagggt
    14821 gacaaatgag agtcccggcg tccatccgct acaacaaccc tggtgccatg tggggc
//
LOCUS       DATGEM010000161        10623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_410980, whole genome shotgun sequence.
ACCESSION   DATGEM010000161 DATGEM010000000
VERSION     DATGEM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410980"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VKQ27_05000"
     CDS             <1..402
                     /locus_tag="VKQ27_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HLY88322.1"
                     /translation="VLVKGDVSGNDPVMVRMHAVDLLDDMTGGPHWIGIHNAMHMISK
                     EGRGVVVLIREHRKTALAERVGQLAAGERRPQRELRDYGIGAQILLDLGVKNMVLLSN
                     SRRTIIGLDGYGLNIVAQKPIPNIVGEGYSG"
     gene            399..881
                     /gene="ribH"
                     /locus_tag="VKQ27_05005"
     CDS             399..881
                     /gene="ribH"
                     /locus_tag="VKQ27_05005"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLY88323.1"
                     /translation="MSTEDAPLPFAPRIGGPPPHLLIMRAPYYREVVDGLTDGAARIL
                     REASATFEQIDVAGAYELPQAIRIALRGTKRFDGFVALGCIIRGETDHYEFICRATMD
                     GIMQVALQFGIALGTGLLTCDTLAQAEARSGKDGHNKGAEAAAAALLQIGVARQLGAA
                     "
     gene            883..1362
                     /gene="nusB"
                     /locus_tag="VKQ27_05010"
     CDS             883..1362
                     /gene="nusB"
                     /locus_tag="VKQ27_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLY88324.1"
                     /translation="MGRAPRRPRTASRVAAVQALFQSEQAQDNPETVIDQFVRHRLGE
                     LPGTGGFEEGRVPDAEVPLFARIVRAAVAQQDTIDSMLANTLPADWPLARIDPVLRAV
                     MRAAAAEMAMAGGAPAKVIINEYLDVARGFFTGAEPGMANAVLDRLARLLRPAEFDA"
     gene            1373..2299
                     /locus_tag="VKQ27_05015"
     CDS             1373..2299
                     /locus_tag="VKQ27_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY88325.1"
                     /translation="MDASDLKVFEAVARYGGISRAAKELHTVQSNVTTRIQSLEQELG
                     TALFERHARGVTLTQAGHRLLPYAAEIRDVLDRARRAAIDDGTPTGPLVIGTLETTVA
                     MRLPRIVAAFASAYPNVDLSLRAGTSIESVVRVLDRELDGAFVAGPLDHAELIQETMF
                     REELVIVTTPSVTTLHDLGRTGPLKILVLRVGCSYRQRLETLLAARGIADVRPMEFGS
                     LDAIIGCVAAGIGVTLLPRAVVAAAARAGEVALHELPHDEAMVETLFVRRHDALVTSA
                     LTAFLDVVRSRTNLAMTNPRNMAAAAYPPAAQ"
     gene            complement(2244..2747)
                     /locus_tag="VKQ27_05020"
     CDS             complement(2244..2747)
                     /locus_tag="VKQ27_05020"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HLY88326.1"
                     /translation="MSKWHIAQMNVGTVRFPTDDPRLAGFMDRLDAINALAEASPGFV
                     WRLQSDQGNATDIILTENPLFIVNMSVWEDVESLFAFVYRSAHQGVMAQRREWFERPD
                     EAYQVLWWIAAGTTPTPQQGLERLRHLQGHGPTPYAFTFRQKFPPPDVIAQPEDMQPQ
                     PYCVGWS"
     gene            2824..4302
                     /locus_tag="VKQ27_05025"
     CDS             2824..4302
                     /locus_tag="VKQ27_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88327.1"
                     /translation="MLRLIVLLTLIALPASAATPIDETVASYHGNPDRAGTYIIPSLT
                     WTAAGAVHRDQPFDGAIEGHVYAQPLYWHPAGAQRGLIIAATESNNVYALDSASGHVV
                     WRTALGAPVPRDALPCGNIDPLGITGTPVIDASASTVYLDAMIDRAGTPQHLVYGLRL
                     TDGSVLAGFPIDVAAGLAAHGIHFIPRVQNQRGALSLLNGRIFVPFGGHYGDCGDYRG
                     VVAAISINPPQLVSGWATRAPKGGIWAPAGLSEADGALYFTTGNTDDAHGWQDGEGVF
                     RVGADLAHRSNPSDYFAPSNWQELDDDDLDLSGVTPLPLTVPGSPRPLMIALGKDGNA
                     YLLDRRNLGGIGGALAVRRAANSAIITSATTYPVGSAALVAYRTRGAACPTGRYVSGI
                     AAIAITPDARLSSAWCSPMNGAGAPIVTTTDGTANPIVWAVGAEGDDGLHAFRGDNGE
                     ELRIGSGTANRMTGLRHFATILVAAGRMYIAGDSRIFAFDLP"
     gene            4361..4765
                     /locus_tag="VKQ27_05030"
     CDS             4361..4765
                     /locus_tag="VKQ27_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HLY88328.1"
                     /translation="MPFDETSRQPGRRPSRNVLGGILDICSTRPMTGFFRNGCCDTSD
                     QDYGSHTVCAVMTAEFLAFSKAAGNDLSTPHPEFGFPGLQPGDRWCLCAPRWQEALDA
                     NAAPRVVLRATQQGALDYCLLADLKKHAVDLA"
     gene            4770..5699
                     /gene="thiL"
                     /locus_tag="VKQ27_05035"
     CDS             4770..5699
                     /gene="thiL"
                     /locus_tag="VKQ27_05035"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLY88329.1"
                     /translation="MSADLPAEFALIARHFRPLAGPGALNLQDDAAVLAPPPGRELVL
                     TCDAMVAGVHFLPDDPPDLIGRKLLRVNLSDLAAKGATPIGYLMTVSAPRDTPDTWYA
                     GFAAGLQQDQRQYGVTLLGGDTTSTPGPISLSLTILGHVAPGQAVHRMGAQPGDGIFV
                     TGTIGDGALGLAVAQGRLSDPTGFLLSRYRLPQPRIGLAIAGIASAGMDISDGLVQDL
                     GHICRASGLLAEIEAARVPLSEPARVAGPDWLPTCLTGGDDYELLLAIPPGREDGIPV
                     TRIGTFQSGPADVIVRGPDGKPMTLARGGWSHF"
     gene            5700..6878
                     /locus_tag="VKQ27_05040"
     CDS             5700..6878
                     /locus_tag="VKQ27_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88330.1"
                     /translation="MNRNVWLLFCCQALMNAVMSGQTVMSALIGHSLAVDKALNTLPM
                     AIQMTATMTASIPAGIVFARLGRKPGFWLGCIGSLLGSLTFALGVWRGNFQIYCAGAV
                     FAGLGFGISQHLRFAAAEVAATEARARAIALVMAGGVLAAIIGPEIVKRTNMMLPPLM
                     FLGTYLCLIVLPLISATLLVFIQLPPATRQLSAPVPFRAIIARPNFLTAVVSSMVGYG
                     TMNLVMASTPLQMLFCGFGVNSSADVIRAHSIAMFLPGFVTGRLINRFGVHPIICAGA
                     VATTLCVATNLSFPPVIPTFMAALMLLGVGWNFMFVGGTTLLATAHDPHERVRVQATN
                     DFIVFGTVACTAFASGAVEATGGWTALNLVVVPPTVIAAALVIWHWMARARLAPAAAQ
                     "
     gene            complement(6872..7369)
                     /gene="bamE"
                     /locus_tag="VKQ27_05045"
     CDS             complement(6872..7369)
                     /gene="bamE"
                     /locus_tag="VKQ27_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HLY88331.1"
                     /translation="MSRRCLSFVPLLLLGCLLLGACSFFEATPQLRGNRVDADSLKEL
                     VPGTSTKADVTSLIGSPTATSTFDDNTWLYITEVTQPQIGRTQAVLSQNVVVLSFNGQ
                     GVLQDVKRLNQDDSIPVSVVARTTPSPGTEATFLQQVFGNIGRFNALGQNPTAPGGGA
                     PAPAY"
     gene            7461..7976
                     /locus_tag="VKQ27_05050"
     CDS             7461..7976
                     /locus_tag="VKQ27_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HLY88332.1"
                     /translation="MLGSRRRRRDTEHSGGQLYAAAVAAARQPFFYATLGVPDTLDGR
                     FDLVALHAFLLVRRLHDHPDLAQAIFDAMFVDMDNNLREIGVGDLSVGKHVRAMWEAF
                     HGRSKAYETPLAANDAHALAAALARNVWRGEAPPGAAEALAERVLAQAAHLEDQPLSV
                     FTSGQARFLSP"
     gene            7973..8467
                     /locus_tag="VKQ27_05055"
     CDS             7973..8467
                     /locus_tag="VKQ27_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLY88333.1"
                     /translation="MSPELHRPIAVDHVGRAGLDVMVEATSDELTALAHRMGLPAVLS
                     LNCAFHLHRDAADRLVARGRLHAAVMQTCVISLEDFPATVDEQFTVLFVPAGKETEDD
                     DPSTPDEISYENGMIDLGEAAAEQLALALDPYPRAPGAELPDSAHDPEDHQFAALAAL
                     KRLQ"
     gene            complement(8576..>10623)
                     /locus_tag="VKQ27_05060"
     CDS             complement(8576..>10623)
                     /locus_tag="VKQ27_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLY88334.1"
                     /translation="AMKLWLSCLALCLTIILVAALPDSLRAQQLDYGALEHVFHEPVT
                     TSATGQPQRASEAPVNMTIITQDDIRRTGATTIPDVLQYVAGVDVRRYGIADEEVGIR
                     GYNAAYNPRLLVLVNGRQVYSDDYGRVFWPTVPVQLAEIRQIEVIEGPNSALYGFNAV
                     SGVINIITFDPLHDRINTATLQGGTQHYWGGSAVATGQIEDRAGLRISVGGYQAQDYG
                     PANLSAVDALNRQNPLVGAFNVDGRLQIAPGVQAYIDGSMSDSRFAEMTFEGSFETNT
                     TRANSIRAGLSADTAIGLLSFSAYRNGQELSIDSSAVSGFPSWVNENVYIVQASDLLK
                     LGADHVVRIGLEYRNNAETSPGFIGGTIGYHVFAGSLMWNWQITPSVALTNAVRVDEL
                     QLRYSGNLAAGSGFTLGNYNSTNFAVPSFNSGLVVELTPEDTIRFMLARGVQVPSLFD
                     FGVQIPFGAVGPVVIAGNPDVHPSTVDNIELDYDRALPAMGSELRFAVFAQRNRDLIS
                     QPFAVPPVLGPTGLPLLLTSNVGSSNAMGAQIGIKGHSDSGFRWNVSYAYVATTDNTV
                     LNRGGLITSAINYANSAPRSVGIAGIGYTRDRWELDLMGRWQSSYQDFRASVSNPFLL
                     EPVEVRNYLSLNARAAYRLTDNFTVHVSAQQFNASQLLQTAGPLVERRVLFGITAGF"
BASE COUNT         1764 a         3653 c         3304 g         1902 t
ORIGIN      
        1 gtcctggtga aaggcgatgt cagcggcaac gatccggtga tggtgcgcat gcacgccgtg
       61 gatctgctgg atgacatgac cggcggaccg cactggatcg gtatccacaa tgcgatgcac
      121 atgatcagca aggaaggccg cggcgtcgtg gtgctgatcc gcgagcatcg caagaccgcg
      181 ctcgccgaac gcgtcgggca gctcgcagcc ggtgaacgcc ggccgcagcg cgaactgcgc
      241 gactatggta tcggcgcaca gatcctgctg gatctcggcg tgaagaacat ggtgctgctg
      301 tcgaacagcc ggcgcaccat catcggcctc gacggctacg gcctgaacat cgtggcgcag
      361 aagccgatac cgaatatcgt cggcgaaggt tattcgggat gagcacggag gacgccccgc
      421 ttccattcgc gccgcgcatc ggcggtccgc cacctcacct gctgatcatg cgcgcgccct
      481 attatcgcga ggtggtggat ggactgacgg atggcgccgc ccgcatcctc cgcgaggcaa
      541 gtgcgacttt cgagcagatc gatgtcgccg gtgcctacga acttcctcag gcaatccgca
      601 tcgcgctgcg cggcacaaag cgcttcgatg ggttcgtggc actcggctgc atcatccgcg
      661 gcgaaacaga ccactacgag ttcatctgcc gcgcgacgat ggacggcatc atgcaggtgg
      721 cactgcagtt cggcatcgcg ctcggcaccg ggttgctgac ctgcgacacg ctggcccagg
      781 cggaagcccg ctccggcaag gatggccaca acaagggtgc cgaagccgcg gctgcggcgc
      841 tgctgcaaat cggcgtggcg cgacaactcg gcgccgcatg atatgggacg cgccccgcgc
      901 aggccacgga ccgcatcgcg ggtcgccgcc gtacaggcac tgttccaaag cgagcaggca
      961 caggacaatc cggaaacggt aatcgatcag ttcgtgcgcc atcgcctcgg cgaactgccc
     1021 ggcaccggcg gcttcgaaga aggccgtgtg cctgatgccg aagtgcccct gttcgcccgc
     1081 atcgtgcgtg cggcggtggc gcagcaggac acgatcgaca gcatgctcgc caacacgctg
     1141 ccggcggact ggccgctggc gcgcatcgat cctgttctgc gcgccgtgat gcgcgccgcc
     1201 gctgcggaga tggcgatggc gggcggcgct ccggccaagg tgatcatcaa cgaatatctc
     1261 gatgtcgcac gcggcttttt caccggcgcc gaaccgggta tggcgaacgc cgtgctggac
     1321 cggcttgccc gcctgctgcg gccggcggag ttcgacgcgt aggaggacag cgatggacgc
     1381 ctccgacctc aaggtgttcg aagcggtggc ccgctatggc ggtatcagcc gcgccgcgaa
     1441 ggaactgcat accgtgcagt caaacgtcac tacgcgcatc caatctctgg agcaggagct
     1501 cggcaccgcc ctgttcgaac gccatgcgcg cggtgtcacc ctgacgcaag ccggccatcg
     1561 gctgttgccc tacgcggcag agatcaggga tgtgcttgac cgcgcccgcc gggccgcgat
     1621 cgacgacggc acgccgacgg gaccgttggt catcggcaca ttggagacaa cggtcgccat
     1681 gcgactgccg cgcatcgtcg ctgccttcgc gtccgcatat ccgaacgtcg atctctctct
     1741 gcgcgccggc acgtccatcg aatcggtcgt gcgggtgctc gaccgtgaac tcgatggagc
     1801 gtttgtcgca ggtccgcttg atcacgccga actcattcag gagacgatgt tccgcgagga
     1861 gcttgtcatc gtgacaacac cgtctgtcac cactctgcat gacctcgggc gcaccggacc
     1921 gctcaagatc ctcgtgctgc gcgtgggctg ttcctatcgc caacggctcg aaaccttgct
     1981 ggccgcgcgc ggcatcgccg atgtgcgccc aatggaattc ggctcgctcg acgccatcat
     2041 cggttgcgtc gccgccggca tcggcgtcac gctgttgccg cgcgcggtgg tggcggcagc
     2101 agcccgcgcc ggcgaggtcg cgttgcacga actgccgcat gacgaagcga tggtggaaac
     2161 gctgttcgtc cgccgccacg atgccttggt caccagcgcg ctcacggctt tcctggatgt
     2221 cgtacgttcg aggacgaacc tcgctatgac caacccacgc aatatggctg cggctgcata
     2281 tcctccggct gcgcaatgac atcgggcggc gggaatttct ggcgaaacgt gaaggcgtat
     2341 ggcgtggggc cgtgcccctg gagatggcgc aggcgttcca ggccctgctg cggcgtcggc
     2401 gtcgtgccgg ctgcgatcca ccacaacacc tgatacgcct catcgggccg ttcaaaccat
     2461 tcacgccgtt gcgccatcac accctgatgc gcactgcgat agacgaacgc gaacagtgac
     2521 tcgacgtcct cccacaccga catattcacg atgaacaacg gattctccgt gaggatgatg
     2581 tcggtcgcgt tgccctgatc ggattgtagc cgccacacga aaccaggcga cgcttccgcc
     2641 aacgcgttga ttgcatccag ccggtccatg aatcccgcca agcgtggatc gtcagtcgga
     2701 aatcgcacgg tacccacgtt catttgcgcg atatgccact tcgacatgac gttctcccgg
     2761 tttgacctcg ttgctacccg aattgccacc ccatatccag gctgaacggc tggagtgctc
     2821 accatgctgc gcctcatcgt gctgctgacg ctgatcgccc tgcccgcttc ggccgcgaca
     2881 ccaatcgacg agaccgtcgc ctcctatcac ggcaatcctg atcgcgccgg cacctacatc
     2941 atcccgtcgc tgacctggac ggcggcgggc gcggtccatc gcgatcagcc gttcgatggt
     3001 gcgatcgagg ggcatgtcta cgcgcagccg ctctactggc atcctgccgg tgcgcagcgc
     3061 ggcctcatca tcgccgccac ggaaagcaac aacgtctacg ccctggattc agccagcggg
     3121 cacgtcgtgt ggcgcaccgc gctgggcgca ccagtgccac gggatgcgct gccctgcggc
     3181 aacatcgacc cgctcggcat caccggcacg ccggtgatcg atgcgtcagc gagcactgtc
     3241 taccttgacg cgatgatcga tcgcgccggc acgccgcagc atctggtgta cggtctccgc
     3301 ctgaccgacg gcagcgtcct cgccggcttt ccgatcgatg tcgctgccgg cctcgccgca
     3361 catggcattc atttcattcc gcgcgtgcag aaccaacgtg gtgccctgtc gctgctgaac
     3421 ggccgcatct tcgttccctt tggtggccac tacggcgatt gcggcgacta tcgtggcgtg
     3481 gttgccgcca tcagcatcaa tccgccgcag ctcgtgtccg gatgggcgac acgcgcgccg
     3541 aagggtggca tctgggcccc tgccgggctg agcgaggccg atggtgcgct gtatttcacc
     3601 actggcaaca ccgacgacgc ccatggctgg caggacggcg aaggcgtgtt ccgcgtcgga
     3661 gcggatctcg cccatcgcag caaccccagc gactacttcg cgccgtccaa ctggcaggag
     3721 ttggacgatg acgacttgga tctgagcggt gtcacaccgc tgccgctgac ggtgcccggg
     3781 agcccgcgcc ccctgatgat cgcactcggc aaggacggca acgcgtatct gctcgaccgc
     3841 cgcaaccttg gcggcattgg cggtgcgctc gcggtccgcc gtgcggcaaa cagcgcaatc
     3901 atcacatctg caacgaccta tccggtgggg agcgccgccc tggtggcata ccggacgcgc
     3961 ggtgccgcat gcccaaccgg acggtatgtg agtggcatag ctgcgattgc gatcaccccc
     4021 gatgccaggc tttcgtcggc ctggtgttct ccgatgaacg gcgccggcgc accgattgtc
     4081 accaccaccg acggcacagc caatccaatc gtatgggcag tcggtgccga gggtgacgat
     4141 ggcctgcacg ccttccgtgg cgataacggc gaggaactcc gcatcggctc cggcacagcc
     4201 aaccggatga ccggtctgcg ccacttcgcc accatcctgg tcgctgccgg acgaatgtat
     4261 atcgccggcg acagccgcat cttcgcgttc gacctgccct gacggtgggt tattccgtcg
     4321 accgccggta taataccgca gcgtcctgaa ggaggaagcg atgccattcg atgaaacctc
     4381 gcgccagcct ggccgtcgtc cgtcgcgcaa cgttctcggc gggatcctgg atatctgctc
     4441 cacccgcccg atgaccggct tcttccgcaa cggctgctgc gacaccagcg atcaggacta
     4501 cggcagccac acggtctgcg ctgtcatgac tgctgagttc ctcgccttca gcaaggccgc
     4561 cggcaacgac ctgtccacgc cacatccgga gttcggcttt ccgggattgc agccgggaga
     4621 ccgctggtgc ctttgcgcgc cgcgctggca ggaagcgctg gatgccaacg ccgcgccccg
     4681 cgtcgtgttg cgcgcgaccc agcaaggcgc gctggattac tgcttgttgg ccgatctgaa
     4741 gaagcacgcc gtcgacctgg cctgatcgcg tgtcggcaga cctgcccgcc gagttcgcgc
     4801 tgatcgcgcg gcactttcgt ccacttgccg gcccaggcgc gctgaacctg caggacgatg
     4861 cagcagtcct cgccccaccg cccgggcgcg aattggtgct cacctgtgac gcgatggtgg
     4921 cgggcgtcca cttcctgcct gacgatccgc ccgatctgat tggccggaaa ctgctgcgcg
     4981 tgaacctctc cgacctcgcg gcaaaggggg cgacacccat cggctacctg atgaccgtct
     5041 cggccccacg ggacacgccc gacacctggt acgctggctt cgccgccggc ctgcagcagg
     5101 accagcggca atacggcgtt acgctgctcg gcggcgacac cacctccact cccggcccga
     5161 tctcgctgtc gctcacaatt ctgggccatg ttgcacccgg gcaggcagtg catcggatgg
     5221 gcgcccaacc tggcgacggc atcttcgtca ccggcacgat cggcgacggc gcgcttggtc
     5281 ttgccgttgc acagggacgc ctgtctgatc ccactggctt tctgctctcg cgctatcgcc
     5341 tgccccaacc acgcatcggc cttgccatcg ccggcatcgc ctccgcgggc atggatatct
     5401 cggatggctt ggtgcaggac ctcggccaca tctgccgcgc gagcggcttg ctggcggaga
     5461 tcgaagcggc tcgcgtgcca ctgtccgagc cggcgcgtgt cgcagggcct gactggctcc
     5521 cgacctgtct gaccggcggc gacgactacg agctgctgct cgccattccg cccgggcgcg
     5581 aggacggcat accagtcacg cgtatcggca ccttccagtc cggccctgcc gacgtcatcg
     5641 tgcgtggccc cgatggcaaa ccgatgaccc tcgcccgcgg cggctggagc cacttctgaa
     5701 tgaaccgcaa cgtctggctc ctgttctgct gtcaggcgct gatgaacgcc gtgatgtcgg
     5761 gccagaccgt gatgagcgcg ctcatcggcc actcgctcgc cgtcgacaag gcgctgaaca
     5821 cgctgccgat ggccatccag atgaccgcga cgatgaccgc ctcgatcccc gccggcatcg
     5881 tcttcgcccg cctcggccgc aagcccggtt tctggctcgg ctgtatcggc tcattgctcg
     5941 gcagcctgac gttcgccctc ggcgtctggc gcggcaactt ccagatctat tgcgccggcg
     6001 ccgtgttcgc cggcctcggc ttcgggatct cgcagcatct ccgtttcgcc gccgccgaag
     6061 ttgcagcgac cgaggcccga gcgcgcgcca tcgccctggt catggcaggc ggtgtcctcg
     6121 ccgccatcat cggcccggag atcgtcaagc gcaccaacat gatgctgccg ccgctgatgt
     6181 tcctcggcac ctatctctgc ctgatcgtcc tgccgctgat cagcgcaacc ctgctcgtgt
     6241 tcatccagct gccgcccgcc acccgccagc tgagcgcacc cgtcccgttc cgcgcgatca
     6301 tcgcccgtcc gaacttcctc accgccgttg tgtccagcat ggtgggctac ggcacgatga
     6361 acctcgtgat ggcctccacg ccgctacaga tgctgttctg cggtttcggc gtgaactcca
     6421 gtgccgacgt gatccgcgcc cactcgatcg ccatgttcct gcccggcttc gtcaccggcc
     6481 ggctgatcaa ccgcttcggc gtccacccga tcatctgcgc tggcgccgtc gccaccacac
     6541 tctgcgtcgc caccaacctg agcttcccgc cggtgatccc caccttcatg gccgcgctca
     6601 tgctgctcgg cgtcggctgg aacttcatgt tcgtcggtgg gacgacgctg cttgcgaccg
     6661 cgcacgaccc gcatgaacgg gtccgtgttc aagccaccaa cgacttcatc gtgttcggca
     6721 ccgtcgcctg caccgccttc gcctcaggcg cggtcgaggc caccggcggc tggacggcgc
     6781 tgaacctcgt cgttgtcccg ccgacggtga tcgcggccgc gctggtgatc tggcactgga
     6841 tggcccgcgc gcgcctcgcg ccagccgctg ctcaataagc cggtgcggga gcaccgccgc
     6901 ccggtgccgt cgggttttgc ccgagtgcgt tgaaccggcc gatgttcccg aacacctgct
     6961 gcaggaacgt cgcctcggtt ccgggcgaag gcgtcgtgcg cgccaccacc gacaccggga
     7021 tcgaatcgtc ctggttcagc cgcttcacat cctgcaggac gccttggcca ttgaagctca
     7081 gcacaaccac gttctggctt agcacggcct gcgtccggcc aatctgcggc tgggtcacct
     7141 cggtgatgta gagccaggtg ttgtcgtcga acgtactggt agccgtcggc gagccaatca
     7201 gcgaggtcac atccgccttg gtcgacgtac cgggcaccag ctccttcagc gaatccgcat
     7261 cgaccctgtt gccgcggagc tggggcgttg cttcgaagaa actgcaggcg cccagcagca
     7321 ggcaacccag cagcagcaac gggacaaacg aaaggcaacg acgagacaag cgggaatcct
     7381 cagccattgg accgatgcgc cgggcgcgct atctcggtgt catggcggcc gcggcgtgtc
     7441 aatcgggagg gatgtgggca gtgttgggtt cgaggcgccg gcggcgtgat accgagcatt
     7501 ctggcggcca gctctacgcg gcggcggtcg ccgcggcccg gcagccgttc ttctatgcga
     7561 cgctcggcgt cccggacacg ctggacggcc gcttcgatct cgtcgccctg cacgccttcc
     7621 tgctggtccg ccggctgcac gaccaccccg acctggcgca ggccatcttc gatgccatgt
     7681 ttgtcgacat ggacaacaac ctccgtgaga ttggcgtcgg tgacctcagt gtcggcaagc
     7741 acgtccgcgc gatgtgggag gcgtttcacg gccgctcgaa agcctacgag actccgctcg
     7801 cggcaaacga tgcgcatgcg ctcgccgcag ccctcgcccg caatgtctgg cgtggcgaag
     7861 cgccccccgg cgcggcggag gcactggctg aacgagtgct cgcacaggct gcccatctcg
     7921 aggaccagcc actctccgtc ttcacctcgg gccaggcccg gttcctcagt ccatgagccc
     7981 ggaactgcat cgcccgatcg ccgtcgatca cgtcggtcgc gccggcctcg acgtcatggt
     8041 ggaggccacc tccgatgagc tcactgcgct ggcgcaccgc atgggtttgc ctgccgtgct
     8101 atcactcaac tgcgcgttcc acctgcatcg cgacgcggcg gatcggctgg tcgctcgcgg
     8161 ccgtctccat gccgcggtga tgcagacctg cgtcatatcg ctggaggact ttcccgccac
     8221 tgtcgacgag cagttcaccg ttctcttcgt ccccgccggc aaggaaaccg aggacgacga
     8281 tccctccacc cccgacgaaa tcagctacga gaacggtatg atcgacctgg gtgaggccgc
     8341 cgccgaacag cttgcccttg cgctcgatcc ctaccctcgc gcgcctggcg ccgaactgcc
     8401 ggattcggcg catgatccgg aagatcatca gttcgccgct ctggccgcac tgaagcgtct
     8461 gcagtaacac gagttcggcc tgccccctcg cccatcggcg gtccgcccgc gcgccgcagc
     8521 gggacgtgcc cccggtccct gccgctcgcc gctcgcatcc ataagaacgt gaacttcaga
     8581 accccgcggt gatgccgaac agtacccgcc gctcgaccag cgggcctgcg gtctgcagca
     8641 gttgtgaggc gttgaattgc tgtgccgaga catggacggt gaagttgtcg gtcagccggt
     8701 aggccgcgcg cgcattcagc gagagataat tgcggacctc gaccggctct aacaggaacg
     8761 gattgctgac cgacgcccgg aagtcctgat agctcgactg ccagcgaccc atcaggtcca
     8821 gctcccagcg atctcgtgta tacccgatcc cggcaatgcc gacgctgcgt ggcgcgctgt
     8881 tggcgtaatt gatggcgctg gtgatcaggc caccccggtt cagcaccgtg ttgtcggtcg
     8941 tcgccacgta cgcgtagctc acgttccagc ggaagccgct gtccgagtgg cctttgatgc
     9001 cgatctgggc tcccatggca ttgctgctgc cgacgttgct cgtgagcaga agcggcagcc
     9061 ccgtcggacc gaggacaggt ggcacggcga atggctggct gatcagatca cgattgcgct
     9121 gcgcgaagac cgcgaaacgc agttctgaac ccatcgcggg aagagcgcgg tcatagtcga
     9181 gttcgatatt gtccaccgta gaggggtgca catcgggatt gccggcgatc acgaccggtc
     9241 caaccgcacc gaacgggatt tgcacaccga aatcgaacag gctgggtact tgcacgccgc
     9301 gcgcgagcat gaagcgaatg gtgtcctcag gcgtaagctc caccacgagg ccggaattga
     9361 aacttggcac cgcgaagttc gtgctgttgt agttgccgag ggtgaagccg ctgccggccg
     9421 ccagattgcc cgaatagcgc agttgcagtt cgtccacccg cacggcgttg gtcaacgcca
     9481 cactgggtgt gatctgccag ttccacatca agctgccggc aaacacgtgg tatccgatcg
     9541 tgccgccgat gaagcccggg gacgtctcgg cgttgttgcg gtattcgagg ccgatgcgga
     9601 cgacatgatc ggcgcccagc ttcaacaggt cgctcgcctg cacaatgtat acgttctcgt
     9661 tgacccagct tgggaacccg ctcacggcgc tgctgtcgat cgagagctcc tggccattgc
     9721 ggtaggcgct gaagctgagc aaaccgatcg ccgtatcggc actcaacccg gcgcggatcg
     9781 agttggccct ggtcgtattg gtttcgaagc tcccctcgaa ggtcatctcc gcgaaccggc
     9841 tgtcgctcat gctgccatcg atatacgcct gcacgcctgg cgcgatctgc aacctgccgt
     9901 cgacattgaa ggcgccgaca agcggattct gtcgattgag ggcatcgacc gcagacaggt
     9961 tggcaggccc gtagtcctgc gcctggtacc caccgaccga gatccgcagg ccggcacggt
    10021 cctcgatctg gccggtggca acggccgagc cgccccaata gtgctgcgtt ccgccctgca
    10081 gcgtcgcggt gttgatccgg tcgtgcaacg ggtcaaaggt gatgatgttg atcaccccgc
    10141 tgaccgcgtt gaatccatac agcgcggaat tcggtccctc gatcacttcg atctgacgga
    10201 tctcggccag ttgcaccgga acggttggcc agaacacgcg gccgtaatcg tccgaataga
    10261 cctgtctccc gttcaccaac accaggaggc gcggattgta ggccgcgttg tagccgcgga
    10321 tgccaacctc ctcgtccgcg attccatagc gccgcacatc gacaccggcg acgtattgca
    10381 gcacgtcggg gatggtggtc gcaccggttc ggcggatatc gtcctgggtg atgatcgtca
    10441 tgttcaccgg cgcctcgctc gcacgctgcg gctggccggt ggcggatgtg gtgaccggtt
    10501 cgtggaacac gtgctccagc gctccgtaat cgagctgctg tgcgcggagg ctgtccggca
    10561 acgccgccac caaaatgatc gtaaggcaca gagccagaca tgagagccag agtttcattg
    10621 ctc
//
LOCUS       DATGEM010000162         7102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_450985, whole genome shotgun sequence.
ACCESSION   DATGEM010000162 DATGEM010000000
VERSION     DATGEM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450985"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VKQ27_05065"
     CDS             complement(<1..288)
                     /locus_tag="VKQ27_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY88335.1"
                     /translation="MTDQLTALRLFVRLAHTGSFSQAAREQKLSQPTASRLIGDLEER
                     LGVTLFTRTTRGVTLSQAGTEYLSHIQPILAALDEADHAARGTGELRGALRV"
     gene            1590..2246
                     /locus_tag="VKQ27_05070"
     CDS             1590..2246
                     /locus_tag="VKQ27_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLY88336.1"
                     /translation="MGIVIGPGFIPMDMVGIQAVFGLMPGAEVYLVWKSLESVEGFPD
                     WWTIPTATFSDCPELDVVAVPMLPPEIQNDREVIDFVAVSGAKARYVIGICNCVLVLG
                     AAGLLNGRRATASYNSLSILPLHGVAEVVSGGGVVADRGVYTAGPGIGSFDAALLVAA
                     QEFGRPAGELAELMIEYDPHPPFGTGSATDADPALIAQFEGIMADMVSYYRAGASSQG
                     "
     gene            2286..3044
                     /locus_tag="VKQ27_05075"
     CDS             2286..3044
                     /locus_tag="VKQ27_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88337.1"
                     /translation="MKLAGNTIFVTGGGSGIGRALAEALHRRGNKVIIGGRRRGHLDA
                     VVAANPGMAAIELDITDPTSIDRAAAQLIAEHPDLNVLVNNAGIMEPDNAAGTLDDAR
                     LLAAVNTNLVGPIRLTSALIEHLKGKEDAVVAYTTSVLAFVPLAFTSVYSATKAALHS
                     YILSQRFMLKSSGVRVIEIAPPWVRTELMNSQEAELAMPLDQFTAETMAALGTDSDEI
                     LVEAARPLRANPGPNEHGLVNGFNAQMLAAVGGH"
     gene            complement(3147..4139)
                     /locus_tag="VKQ27_05080"
     CDS             complement(3147..4139)
                     /locus_tag="VKQ27_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLY88338.1"
                     /translation="METLDTAVSSGPPLICFYVFPGHAMLDVAGPMSAFVMPLRLGDP
                     GRYATAVLSRTGGAVPSYEGVNVTTLADPGRPIDTFIVVGGPGVFGLDKSEVDAVRAL
                     AKTARRVASVCTGAFLLASAGILDGRRATTHWAEAEQLARGFPSVQVESDRIYVQDGP
                     VWSSAGVTAGIDLALALIEADYGADLARTIARNLVVPFRRRGGQSQYSEMLALDPLSD
                     RIARTLAFAQAHLSENLTVERLATIACLSPRQFGRAFHGATGATPANAVERLRVEAAK
                     ARVESSIEPIEAIAASVGFVDPERMRRAFIRRFGQSPQALRRAVRDRVALREAL"
     gene            complement(4251..5831)
                     /locus_tag="VKQ27_05085"
     CDS             complement(4251..5831)
                     /locus_tag="VKQ27_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLY88339.1"
                     /translation="MRTLRISAHDRTRCNSRPDDREGRKHRPQRVVQASDGAVRHPAQ
                     TMGRARARRSAIRCESPSLRTNAMNSFQHLFDEQKALFASSVTRSRAWRLEQLDRMGR
                     MIKENEAALQHAVARDFKTASQEYIFETAATFLETEYQKSQLETWMTPSEAPVPKALA
                     RTGHKAMVYRDPYGVVLVIGPFNGPLLLLLRPAITALAAGNTCILKLSPALSATSAVL
                     LDLVPKYFDPRVVATVAGDREETTELLKLPFDFIFFTGSTKVGKVVARAAAEHLTPVL
                     LELGGQNPAVVDRTANIPDAAKKIVWGATAWGGQWCTSPGYAYVDASIAEEFVAEAKK
                     AIVDLYGSDPKNNPDYSRIISAKEVKRLSALIDPAKVVSGGGADPEARYLDPTILFPI
                     SWDDEIMADEVFGPILPILTYSNLDEAFDSIAAKPHPLSAFIFSRDQATIDRFIGELS
                     FGGGAVNQTNIHLFIETMPFGGVGPAGMGHYYGRDGYNMLTHAKSILVSPPDVAIDHL
                     FPPYTDEKNRELAIWFDY"
     gene            complement(5961..6560)
                     /locus_tag="VKQ27_05090"
     CDS             complement(5961..6560)
                     /locus_tag="VKQ27_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY88340.1"
                     /translation="MALGYEDLSDHDELTRAPTMAVLAGKLAARRRDCAPVAGKSTLN
                     RLELSGPIPSRYHKIGHDPAAIEGLFVDLFLEAHTRAPKQIILDLDVTDARPAYSHSC
                     ACFAQNFVLANWPWLGVAAELEGFSTSLLLSSATSFTELAGPTAATAAWARPSTKPRT
                     EYEPRSSRNPEPPDRRLHVRGRALRNFGTTDVWRAVPLR"
BASE COUNT         1285 a         2251 c         2309 g         1257 t
ORIGIN      
        1 cacccgcagc gcacctctca gctcaccggt gccgcgggcg gcatgatcgg cctcatcgag
       61 tgccgcgagg atcggctgga tgtgcgagag atactctgtg ccggcttggg agagcgtgac
      121 gccccgcgtc gtgcgcgtga agagcgtgac gccaaggcgc tcctccagat cgccgatcag
      181 gcgcgacgcc gtgggctgtg acagcttctg ttcgcgggcc gcttgcgaga agctgcccgt
      241 gtgcgcgagg cggacgaaga ggcgaagggc ggtcagttgg tcggtcattg tgcttacgag
      301 attgcactgc aacgtcgcac tttggtaggc gactgctctc ctgatcgaag caacaccgaa
      361 gaagcgcaat gaccacctca accggcagaa gctgggtggg cggcgcgccg tccgcccggc
      421 ctcgtcagac aaatcccacc acgcccgtgg acgctaccgt aggtcaacgg accggcgggc
      481 gttcgacagc cgcatccgcg tagagcgagg caacgcgcat gcgagcatcg cggcgcatct
      541 ttcgccatgc tcccgggctg acgccaacat accgtcgaaa taccttcgac aaatgggcct
      601 agtccgccaa tccgcactcg agcgctactt cggagagcgg catgtccgtg gtgaggagca
      661 ggtctcgcgc cctaccaatt cttcgaagag tgatgaatgt gtaggggctc tcgcccctgg
      721 taatccggaa ggcgcgagag aaatgactgg tgctcagcct cgtcgcgtcg gccagttgct
      781 gcagcgctat cttctccgcg agattctcgt cgacaaaagt gagagcgcga gcgatttgcc
      841 agggcgcgag gaggcagatg ttagattctc gatgcccttg ggtgccgatg gcagccgggc
      901 gcttcccagt caggaggcct atagcctctt gcatgcagga ttgtgcccga ctggggtcac
      961 tccagagcat tttcccggct tcctggatga ggcgtgcggc ccacaacagc gcttccttgg
     1021 ggcaggccgg gtcggagcgg agcgccgcag cgcccggacc tgttggtcgg attgcatggg
     1081 gcttgctggg atgcgcgcca gcggattgtc cttgagcatg catgtcggac ctctgcgctg
     1141 tcggtgctta caaaatctgc aaaacgagat gtgtcatgcg tcggggcggc cgaaccggca
     1201 gcagtgtcgg ctcgaccgga atgctgcctc atttcaactg cgcctgaacc ctgggcagcc
     1261 gggccattcc tttagcgtca ggccaaggct aagcgcgcgc gccgccgcga cgtgacgtcg
     1321 aaaaaacctc aaaataggtc gcttccactg acgccagacg acgcgccacc tcacggctca
     1381 ggccggccgc gcaggtcgaa ctgatgcttc aaaccatggc gaaattcgag gggaatacgt
     1441 cgttttagac atcagctcgg attgttatct gtcgctcatc ttccttgaac acgagtgcga
     1501 cgatgagcaa gcgaccgcct cagaccgacc tgccgtctct tgtcaggttc agagccgcga
     1561 cgtcctcggc cggcgtcagc ccgttccgtg tgggcatcgt catcggtccc ggcttcatcc
     1621 ccatggacat ggtcggcatc caggcagtct ttggcctgat gccgggtgcg gaggtctatc
     1681 tggtctggaa gtcgctcgag tcggtcgagg gctttccgga ctggtggacg ataccgacgg
     1741 cgaccttttc cgactgcccg gagttggacg tcgtcgccgt gcccatgctg ccgcctgaga
     1801 ttcagaacga tcgcgaggtc atcgacttcg tggcggtcag cggcgcgaag gcccgctacg
     1861 tcattggcat ctgcaactgt gtgctggtcc tgggtgccgc cggactgctc aacggccggc
     1921 gcgcgacggc cagctacaac tcgctgtcga tcctgcccct tcacggggtg gcggaagtcg
     1981 tatcgggcgg gggcgtggtc gccgacagag gcgtctacac cgccgggccc ggcatcggca
     2041 gcttcgacgc ggccctgctc gtggcggcac aggaattcgg acgaccggcc ggcgaattgg
     2101 ccgaactgat gatcgagtat gacccgcatc cgcctttcgg caccggaagc gccaccgacg
     2161 ccgatcctgc gctgatcgcg cagttcgagg ggatcatggc tgacatggtc tcctattacc
     2221 gggccggggc ttcgagccag ggctgagacg gcgcgtgatc cccaactttc ccaaggagag
     2281 ccatcatgaa actcgcaggc aacaccatct tcgtcaccgg cggcggttcc ggcatcggcc
     2341 gggcgctggc cgaggcgctg caccggcgcg gcaacaaggt catcatcggc ggccgccggc
     2401 gcggccatct cgacgcggtc gtcgcggcca acccgggcat ggcggcgatc gagctcgaca
     2461 tcaccgatcc gaccagcatc gaccgggcgg cggcccagct tattgccgag catccggacc
     2521 tcaatgtgct cgtcaacaac gccggcatca tggagccgga caatgcggcc gggacgctcg
     2581 acgacgcgcg gctgctcgcg gctgtcaaca ccaacctcgt cggcccgatc cgcctgacct
     2641 cggcgctgat cgagcatctg aagggcaaag aggacgcggt cgtcgcctat acgacatcgg
     2701 tgctggcctt cgtgccgctc gcattcacca gcgtctattc ggcgaccaag gcagcgctcc
     2761 actcctacat cctgtcgcag cgcttcatgc tgaagtcgag cggcgtgcgc gtgatcgaga
     2821 tcgctccgcc ctgggtgcgt acggagctga tgaacagcca ggaggccgaa ctggcgatgc
     2881 cgctcgacca gttcacggcc gagaccatgg cagcgctcgg cactgattcc gacgagatcc
     2941 tggtcgaggc ggcgcggccg ctgcgcgcca atccggggcc gaacgagcat ggcctcgtca
     3001 acggcttcaa tgcccagatg ctggcggcgg tcggcggcca ctaaagccct ccgccatcgt
     3061 ggcgaggccg cgcagggcgc ggttttgccc gatggcggtt caaggccttt ccagggcagg
     3121 gcctcgtcga gcggcactca cgaggcttat agcgcctctc gcagcgcaac ccggtcacga
     3181 acggcgcggc gcagggcttg gggcgactgt ccgaagcgcc tgatgaaggc tcgccgcatc
     3241 cgctcgggat cgacgaatcc aaccgaggcc gcgatggctt cgatcggctc gatgctgctc
     3301 tcgacacgcg cctttgctgc ctccacgcgc aatcgctcga ccgcgttcgc cggcgtggcg
     3361 cccgtcgcgc catgaaacgc gcgcccaaac tgccgcgggc tgaggcacgc aatcgtcgca
     3421 agccgctcca cagtcaggtt ctccgacaaa tgcgcctggg cgaacgccag cgtgcgcgcg
     3481 attcgatccg acagcggatc gagcgccagc atctcggaat attgggactg gccgccgcgt
     3541 cgcctgaacg gcacgactaa gttccgggca atcgtgcgcg ccaaatcagc cccatagtcc
     3601 gcttcgatca gagccaatgc gaggtcgatg cccgctgtga caccggccga tgaccagacc
     3661 ggcccatcct ggacgtagat ccgatcggac tcgacctgca cggaggggaa gccccttgcg
     3721 agctgttcgg cctcggccca atgggtggtc gcgcggcgcc cgtccagaat gcccgccgat
     3781 gccagcaaaa aggctccagt gcaaacactc gccacacgcc ttgccgtctt tgcaagggcg
     3841 cgcacggcat cgacctcgga cttatccaac ccgaagacgc caggcccccc gacaacgatg
     3901 aacgtgtcga tcgggcgccc cgggtcggcc agggtcgtca cgttgacacc ttcataactt
     3961 ggaaccgcac cgccggttcg ggacaacacc gccgtcgcgt agcgtcctgg atcacccaag
     4021 cgtaagggca ttacgaacgc agacattggc cctgcgacgt cgagcatcgc gtggcccgga
     4081 aatacataga agcaaatgag cggcggcccg gaagagaccg cggtgtcgag cgtctccatt
     4141 gcaccatgat agcatgcgga aactggtatt cccatggcgg cggaccctca gagccggggc
     4201 gcgacgcaag tcgcaccgcc ggcctggatt ggacgccttt cctcgcgtcc tcagtagtcg
     4261 aaccagatgg cgagttcgcg gttcttttcg tcggtatatg gggggaacag atggtcgatg
     4321 gcgacatcgg gcggcgacac caggatcgac ttggcatggg tcagcatgtt gtacccgtcc
     4381 ctaccatagt aatggcccat gccggcgggc ccgacaccgc caaagggcat cgtctcgatg
     4441 aatagatgga tgttggtctg gttgaccgcg ccgccgccga acgaaagctc accgatgaag
     4501 cgatcgatcg tcgcctggtc tcggctgaag atgaacgccg acaaggggtg gggcttggcc
     4561 gcaatgctgt cgaacgcctc gtccaggttc gaataggtca ggatcggcag aatggggccg
     4621 aaaacctcgt ccgccatgat ctcgtcgtcc caggaaatgg gaaaaaggat tgtgggatcg
     4681 aggtaacggg cctccggatc cgctccgccg ccggagacga ccttggcagg atcgatgagc
     4741 gcggagagcc gcttgacctc cttggcgctg atgatccgcg aatagtccgg attgttcttg
     4801 ggatcgctgc cgtagaggtc gacgatcgcc tttttcgctt cggcgacaaa ctcctccgcg
     4861 atcgacgcgt cgacgtaagc atagccgggc gaggtgcacc attggcctcc ccaggccgtc
     4921 gctccccaaa cgatcttctt ggccgcgtct gggatattgg cggtccggtc gacgacggcg
     4981 ggattctggc cgccgagctc gagcagaacg ggcgtcagat gctcggcggc ggcgcgcgcg
     5041 acgaccttgc cgaccttcgt gctgcccgtg aagaagatga agtcgaatgg caacttcagc
     5101 aattcggtcg tttcctcgcg atctcccgcg acagtggcga ctacacgcgg atcgaagtac
     5161 ttcgggacga gatcgagcag gacggccgac gttgcggaca gcgccgggct gagcttgagg
     5221 atgcaagtgt tgcccgcggc gagggccgtg atcgccggac gcaacagcag cagtagtggc
     5281 ccgttgaatg gcccgataac cagcaccacg ccataggggt cccgatagac catggccttg
     5341 tggccggttc gggcgagcgc cttgggcacg ggtgcttccg atggcgtcat ccaggtttcg
     5401 agctggctct tctgatattc ggtctcgagg aaggtggcgg cggtctcgaa gatatattcc
     5461 tggctcgccg tcttgaaatc gcgggcgacg gcatgctgca aggccgcctc gttctccttg
     5521 atcatccgcc ccatgcggtc gagctgctcg agccgccatg cgcgggaccg ggtgacactg
     5581 ctggcgaaaa gcgctttctg ctcatcgaag agatgctgga agctgttcat ggcattggtc
     5641 cttagactgg gggattcgca tcgaattgct gagcgccggg cacgggctcg acccatggtc
     5701 tgcgccgggt gacgaacagc tccatcgctg gcctgaacca ctcgttgtgg tcgatgcttc
     5761 ctgccttcac gatcatcagg tcgggagtta catctggttc tgtcgtgagc ggagatccgc
     5821 agcgtgcgca actgcggcgt cccacccgca taccgctcgc gccaacgtcc tcgaagaccg
     5881 tcgtttcccc ctcaagccgg aacgtcgaac gcttgatgaa aacaatggtg gaaaacgcgc
     5941 tgcccgattg tggtcggcat ctatcgcagt ggcacagcgc gccaaacatc ggtcgttccg
     6001 aaatttcgta acgcacggcc ccgcacatgc agccgccggt caggtggctc gggatttctg
     6061 gaagatcgtg gctcatactc cgtcctcggc tttgttgaag gccgcgccca tgcagcggtt
     6121 gctgcggtcg ggccggccag ctcagtaaag ctggtggcgg aagagagaag gagtgaggtc
     6181 gaaaaaccct ctaattcggc cgcgactcca agccacggcc agttggcgag cacgaagttt
     6241 tgcgcaaagc aggcgcaact gtggctgtaa gccggtcggg cgtccgtcac gtcgagatcg
     6301 aggatgatct gcttcggcgc gcgcgtgtgg gcctccagga acagatcgac gaacagcccc
     6361 tcgatcgcgg ccggatcgtg accgatcttg tgatagcggg agggtatggg cccgctcagt
     6421 tccagtcgat tgagcgtcga tttgcccgcg accggcgcgc agtcccgccg ccgcgccgcg
     6481 agcttgccgg ccagcacggc catggtgggt gcacgcgtca attcgtcgtg gtcgctcaga
     6541 tcctcgtagc cgagcgccaa tcgaacaagc aagggcgcgg gcagcggaac aagttactcg
     6601 gtgcaggcac ctgccgccat gcgcagccgc gaatgtccga cttggcctgc caactgccta
     6661 aaccggtcag gctgctctcg gcccgtgcaa gccgaaatgc ggcccgtcgg caacgcgccc
     6721 atggctgcca gtcgaacgtc ttgacgcctt gctcggtagc ggacgttcac ttaaggagcc
     6781 aggacgacaa tccagcctgg cggtcgctgc acgtcggcta aaaatactgg tcgtcaggac
     6841 gtcgcgcatg gatatcggcg gtcgccctcg ggatcccgct tagtcacaac agccgtgatc
     6901 acgcaccgaa ggggggccac ttgccagatg gccacaacgc gctgatttgg gtagcgaacc
     6961 ccgccccggc gcggaaggcg cagaggcggg gaggtcgaag gcgctcggac gtctatttcg
     7021 ccagcgcctt ggtgagcgcc gcgttggcga gtgcgatggc gctgcgggta gccggcgttt
     7081 cggccagcgc gttcagcatg ac
//
LOCUS       DATGEM010000163         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_410627, whole genome shotgun sequence.
ACCESSION   DATGEM010000163 DATGEM010000000
VERSION     DATGEM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410627"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2547)
                     /locus_tag="VKQ27_05095"
     CDS             complement(<1..>2547)
                     /locus_tag="VKQ27_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY88341.1"
                     /translation="KDLYDLAGKLRTELIKVDNVGTSYLVGTGPEQIQVEPDPEKLSL
                     FGVTLQQLIAKVRGANQEFVAGYVRDTGLTRTLAAGQTLRGVPDIGLLLLTTRDGRPV
                     YLRDVAKVLVGPSTAEDRVWNLTPNGKGWDRVPAVSVALSKRAGSNAVVVAQDILQRV
                     DSLQVRLIPPDIRVQVTRDYGKTADDKANELLFHLALATVSIVVLIAFAVGWREAVVT
                     LVVIPTTILLTLFSARLMGYTINRVSLFALIFAIGILVDDAIVVVENIARHWAMRDGR
                     SRMQAAIEAVAEVGNPTVVATLTVVAALLPMLFVSGLMGPYMAPIPANASAAMLFSFF
                     VAMVVAPWLLCKLHPERGRVDDVDFLPVTTPGIEHDLAAHHEGRLGRIYRAVATPLLR
                     SRARAWTFLLLVAAAMIAACVLFYTENVTVKLLPFDNKSELAVELNLPEGSSLEDTER
                     TLFAAADIVRGLPEVQSVQAYAGTAAPINFNGLVRHYYMRKSPELGDLQINLLDKSGR
                     SRESHAIALDLRKRLTALPLPHGATLQVVEVPPGPPVLATLLAEIYGPDAKTRLAVAE
                     EVKKLFKSVPYIVDVTDSYGHPRPRLRISIDQDELEYFGVEQNDVYDTIQSLFGGIPI
                     GYSHRGEERDPIEIRVGLPKRDLTWDQALASTPVPANTVPGSKTVVELGQVVHADREM
                     GSRVLFRRDGHFADMVMGELAGEFEAPIYGMLAVDKVIEQHDWGKLPKPAIGFRGQPG
                     DESHPTLLWDGEWEITYVTFRDMGAAFMVAMLGIYILVVAQFRSFKLPLVILTPIPLT
                     LIGIVPGHWLFNAPFTATSMIGFIALVGIIVRNSILLVDFVRHGGETGRSL"
BASE COUNT          480 a          780 c          844 g          443 t
ORIGIN      
        1 tagactccga ccggtttcgc cgccgtgccg cacgaaatcc accagtagga tcgagttgcg
       61 cacgatgatt ccgacgagcg cgatgaagcc gatcatggag gtcgcggtga atggagcgtt
      121 gaacagccag tggccgggaa cgatgccgat cagtgtgagc gggattggcg tcaggatcac
      181 caggggcaat ttgaagctgc gaaactgtgc cacgacaagg atatagatgc cgagcatcgc
      241 caccatgaag gccgcgccca tgtcgcggaa cgtgacgtag gtgatctccc attctccatc
      301 ccacagcagc gtgggatgtg attcgtcccc tggttggccg cggaaaccga tcgcgggctt
      361 ggggagcttg ccccaatcgt gttgctcgat caccttgtcc acagcgagca tgccgtagat
      421 cggggcctcg aattcgccgg cgagctcacc catcaccatg tcggcgaagt gcccatcgcg
      481 acggaacaaa acgcgcgatc ccatttctcg gtcggcatgc acgacctggc cgagctcgac
      541 caccgtcttg ctgccgggca ccgtgttggc cggcacgggc gtggaggcca gcgcctgatc
      601 ccatgtcagg tcgcgcttcg gcaggccgac acggatctcg atgggatcgc gttcctcgcc
      661 acgatgcgag tagccgatgg gaatgccgcc gaacaatgac tggatcgtgt cgtagacgtc
      721 gttctgctcg acgccgaaat attcgagctc gtcctggtcg atgctgatgc gcaggcgcgg
      781 acgcgggtga ccgtaggagt cggtgacatc gacaatatac ggcaccgact tgaacagctt
      841 cttcacttcc tcggccacag cgaggcgggt ctttgcgtcc gggccataga tctcggcgag
      901 cagggtcgcc agcacgggcg gtcctggcgg cacttccacc acctgcagcg ttgcgccatg
      961 cggcagaggc agcgcagtca gccgcttgcg caggtcaagc gcgatcgcgt ggctttcgcg
     1021 cgagcggccg gacttgtcca ggagattgat ctgcagatcg cccagttcgg gcgacttgcg
     1081 catgtagtag tggcgcacca ggccgttgaa attgatcgga gcggctgtcc cggcataggc
     1141 ctgcacggac tgcacctcgg gcaggccacg cacgatgtcg gcagcggcga acagcgtgcg
     1201 ctcagtgtct tccaggctgc tgccctcggg taggttcagt tccaccgcga gctcggactt
     1261 gttgtcgaat ggcagcagct tcacggtgac gttctcggtg tagaacagca cgcaagcagc
     1321 gatcatggcc gcggcaacga gcagcaggaa cgtccaggct cgggcacgcg atcgcagcaa
     1381 cggcgtggcg acggcgcgat agatgcggcc cagtcgacct tcatggtgag ccgccagatc
     1441 atgctcgatg cctggtgtgg taacgggcag gaaatccacg tcgtctacgc gtccccgttc
     1501 cgggtgaagt ttgcacagca gccacggtgc cacgaccatc gcgacaaaga acgagaacag
     1561 catcgcggcc gacgcgttcg ccgggatcgg cgccatgtac ggccccatca ggccagacac
     1621 aaacagcatc ggcagcagcg ccgcgaccac tgtcagtgtc gcaacgacgg tcgggttgcc
     1681 gacttcggcg acagcctcga tcgcggcctg catgcgcgag cggccgtcgc gcatcgccca
     1741 gtgacgtgcg atgttctcga ccacgacgat cgcatcatcc accaggatgc caatggcgaa
     1801 gatcaacgcg aacaggctga cgcggttgat cgtgtagccc atcagccggg cggagaagag
     1861 cgtcagcagg atggtggtgg ggattaccac aagtgtcacc acagcctcac gccaaccgac
     1921 ggcaaaggcg atcagcacga cgatggacac ggtggccagg gcaagatgaa acagcagttc
     1981 gttggccttg tcgtccgccg tcttgccgta gtcacgcgtg acctggacgc ggatgtcggg
     2041 cgggatgagc cggacttgga gtgagtcgac gcgttgcagg atgtcttgcg ccaccaccac
     2101 ggcgttggaa ccggcgcgct tggacagagc gacgctcaca gccggcacgc ggtcccagcc
     2161 tttgccgttc ggcgtgaggt tccacacgcg gtcctcggcc gtgctggggc cgaccaacac
     2221 cttggctacg tcgcgcaggt agaccgggcg gccgtcgcgc gtagtgagca gcagcaggcc
     2281 gatatccggc acgcctcgca gcgtctggcc ggcggcaagg gtgcgtgtca ggccggtgtc
     2341 tcgcacgtat ccggcaacga actcctggtt cgcgccgcgt accttggcga tgagctgctg
     2401 gagggtgacg ccaaacagcg acagtttctc gggatcgggc tcgacctgga tctgttcggg
     2461 gccggtgcca acgaggtaac tggtgccgac gttgtctacc ttgatgagtt cggtgcgcag
     2521 cttgccggca aggtcgtaaa ggtcctt
//
LOCUS       DATGEM010000164        11964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_409914, whole genome shotgun sequence.
ACCESSION   DATGEM010000164 DATGEM010000000
VERSION     DATGEM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11964
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409914"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /gene="dnaE"
                     /locus_tag="VKQ27_05100"
     CDS             complement(<1..2600)
                     /gene="dnaE"
                     /locus_tag="VKQ27_05100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HLY88342.1"
                     /translation="MSHADFVHLRVHSAYSLSEGAIKADKIPALARDANMPAVAITDT
                     ANLFGALEFSQACAAKGIQPIIGCQIELTRADNPRLPPDPIVLLAQNPTGLGNLQRLS
                     SRGFLDTEPGLRAQLSIDVITEHAAGLILLTGGATGPLARLLGEGQKHEAERLLARFA
                     EGFPDRVAMELHRHNLQSERAVEPGLLALADAAGIPLVATNDCYFPTPDKYEAHDALL
                     AIAEGRLLSEPDRRRVSPDHWFKPAPDMRMLFADLPEACDNTIAIAQRCAVMMETRKP
                     LLPVSPKVRAGNTEEETVRVMAIEGLDRRMDAMGADEGTRGRYRERLDYELDVICKMG
                     FPGYFLIVADFIQWAKAQGIPVGPGRGSGAGSVAAWALTITDLDPIRFNLLFERFLNP
                     ERVSMPDFDIDFCQEGRDAVIDYVRREYGGDRVAQIITFGKLQARAAVRDVGRVLGLP
                     FGQVNKVAELIPNNPAHPVNLQEAIAGEPRLRMMRDEDEGIARLLEIALQIEGLYRHA
                     STHAAGVVIGDRPLIELVPLYRDPRSDFLVTQYSMKYVEQAGLVKFDFLGLTTLTILQ
                     RAVGMLHGLGIEVDLDKVPLDDPKTYEMLQRGDAGGVFQFEGQGMRDALRQMRPDRFD
                     DLIALGALYRPGPMANIPDYCRRKHGESWEAPHPLIHDILEETYGIMVYQEQVMQIAQ
                     KMAGYSLGAADLLRRAMGKKIRSEMEAQREIFVQGATANGIAKAKAIEVFDLMAKFAD
                     YGFNKSHAAAYALVAYHTAWMKANHAEVFIAACMSLALGNTDRLAALREEASRSGIRI
                     LPPDINNSGADFTVERIDDGKLAIRYALAAVKKVGFSAMQALTQARGDTPFTGLADFA
                     ARVDPRQLNR"
     gene            complement(2650..3336)
                     /locus_tag="VKQ27_05105"
     CDS             complement(2650..3336)
                     /locus_tag="VKQ27_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88343.1"
                     /translation="MSDPLVLRDVRRIYRTEAGELPVLTGADMVLHAGEIVALVAPSG
                     AGKSTLLHLAGLLEKPDGGSVLVDGRDAGALSDAQRTAIRRDTIGFVYQFHHLLGEFT
                     ALENVVLPQMIARTPRRAAAQRAMALLSSFGLQDRADHLPGKLSGGEQQRVAIARALA
                     NEPRVLLADEPTGNLDVATAAVVFDELLMMVRREKLAALIATHNPELAARMDRTVTLR
                     DGRLVMLPAA"
     gene            complement(3329..4582)
                     /locus_tag="VKQ27_05110"
     CDS             complement(3329..4582)
                     /locus_tag="VKQ27_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HLY88344.1"
                     /translation="MFGPFERAVAGRYLRARKGERFVSIIAIFSLIGIALGVATLIVV
                     TSVMSGFQTELESRILGINGHISIEAFAGSQIDNYPPLLTQIRAIPGIVSALPVLDGQ
                     ALLTTDRGGSKGGLVRGMTLEDLRALHPVSDHIVAGKLDAFTGDDALIVGIGLAQSYQ
                     LRIGDSLTVLSPQGAATAFGTVPRVKAYKVVAIFDAGLYDYNNNVVFMPLAAAQVFFQ
                     KPDGITSIEIRVTDPDHVDALVPPLQKAIGDRHVWMRDWRHANDTIISVLQVQKDTMF
                     IVLGMIVLVAAFNVISSLIMMVKDKTRDIAVLRTLGADAGAVLRIFLMCGAFVGVSGT
                     IIGTIIGIVFCRNIVAIQHFVEAVTGGQVFDQTVFMLTALPNSIDWGDVIRVVILALV
                     LSLLATLYPSWKAARTDPVEALRHE"
     gene            complement(4586..5893)
                     /locus_tag="VKQ27_05115"
     CDS             complement(4586..5893)
                     /locus_tag="VKQ27_05115"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLY88345.1"
                     /translation="MRLSRSFIPTMKETPAEAQIASHRLMLRAGLVRQTSAGIYAWLP
                     LGYRVLKNIERIVREEQNAAGAQEVLMPTIQSAELWRESGRYDDYGKEMLRIRDRHER
                     DMLYGPTNEEVITDLFRQSVHSYRELPQILYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDMDYAGAVVSYRTMMLAYMRTFQRMGLKAIPMAAESGPIGGDLSHEFIILASTGE
                     SQVYYDTGFEDIDWVNAKFDYNAQDLEAFFKQMTSMYAATDEKHDPAKWDQVSAARKR
                     EGRGIEVGHIFHFGTKYTKAMNATVTAADGSKVFPEMGSYGVGVSRLVGAVIEASHDE
                     AGIIWPDSIAPFKAAILNLRRGDGACDAMCEALYGRLGSAALYDDREERAGAKFADAD
                     LMGHPWQIIVGPRGAAAGKVELKRRATGERSDVTPDEALARIG"
     gene            complement(6281..6541)
                     /locus_tag="VKQ27_05120"
     CDS             complement(6281..6541)
                     /locus_tag="VKQ27_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HLY88346.1"
                     /translation="MSWFTGVVLYVLIWWTALFAVLPIGTRPVEEADEATGWRGAPQK
                     PQLLRKVIITTLVAAVVWFGAWLLITSPYISFRHGWFAAPQD"
     gene            complement(6538..8184)
                     /locus_tag="VKQ27_05125"
     CDS             complement(6538..8184)
                     /locus_tag="VKQ27_05125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLY88347.1"
                     /translation="MDFDNSDLAFLPLGGTGEIGMNLNLYRCNGRWLAVDCGIGFGGA
                     AHPEVDVMMPDPAFIADRADKLVGLVITHAHEDHLGAVAWLWPQLRCPVYATPFAAAV
                     LRRKLNEVQLLNQVKLHVVAPGAPIDLPPFRLKFLRVTHSIPEAQALVIDTPHGLLLH
                     TGDWKLDPAPLIGEPTNEAEFEALGARGVLAMICDSTNAMVEGHSGSESDVRQTLSVL
                     LRDLRGRVAVTCFASNVARLESVALAAHDAGRSVALVGRSLRNIDAAARECGYLRDLP
                     PFLTEDDVDDVPDDNILMMVTGSQGEPRSALARIAMDTHPRVALGQGDTVIFSSRMIP
                     GNERAIVTVQDNLVRRGVQLMTDADHMVHVSGHPARDELRRLYRLVKPRFSVPTHGEW
                     RHLSAHAALARDAGAQPILLEDGDVLSLSPERAEVTDSAPVDKLVLDGSRLTPIGGGV
                     MTARRRMLFNGVVVASLAVDANGRLRGAPRISAPGLFDNDDAELDRIHTEFADAIDDL
                     PQSLRRDDDALNDAARAALRRALGRRLQKRPLVDVHLLRV"
     gene            complement(8197..9006)
                     /locus_tag="VKQ27_05130"
     CDS             complement(8197..9006)
                     /locus_tag="VKQ27_05130"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLY88348.1"
                     /translation="MLLVIDCGNTNVVFAVRDGAAWRGTWRIRTDAQRTSDEYSVWLL
                     SLLEHSGLKREDISSAVIGTVVPAALYHLRRICRDWFNVEPLIARSTLDWGFDIRVVN
                     AAEVGADRLLNTLAAHRRFGGPLVIVDFGTATTFDVVEKDGAYLGGVICPGINLSLEA
                     LHQAAAQLPRIGIARPQAVIGRQTVPAMQSGIYWGYVAMVEGLVARIKAEYGGPMKVV
                     ATGGLASLVAEGTMVIEQIEPDLTLDGLRLLAERNPVPTLRADRARLIEQD"
     gene            complement(9089..10510)
                     /gene="nuoN"
                     /locus_tag="VKQ27_05135"
     CDS             complement(9089..10510)
                     /gene="nuoN"
                     /locus_tag="VKQ27_05135"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HLY88349.1"
                     /translation="MNWTVALPEIVLSIVAMAVLIFGVLRKEDSFLLSSMFALGGFLL
                     AALLVLTGDRGLGYHGQFVSDAFSAFVKLLILAGAALSLMLSLDYNRKNHIARFEFPV
                     LMVLSVTGMMVMTSASNMMTLYLGLELQSLALYVLAAFARDELRSSEAGLKYFVLGAL
                     ASGLLLYGISLVYGFSGTMDFAALARALARPEAASPGLIVGVVFVLVGLAFKISAVPF
                     HMWTPDVYEGSPTPVTVFFSTAPKVAAFALLLRVMATPFGHLVGAWQELIVLISIASM
                     ILGALAAIGQTNIKRLMAYSSIGHMGYALIGLAVGTPEGIRGVLVYLVVYVFMSAGTF
                     ACIIAMQRRGRSLELISDLNGLGRTDPALALAMAVFMFSMAGVPPLSGFFGKLYVFLA
                     AVQGGMWTLAIIGVLTSVVGAYYYLRVVKVMYFDAPEEAFDPRPASVSFVAAATGLFT
                     TFFIVFPAPFVGAAQAAAKVLFG"
     gene            complement(10507..>11964)
                     /locus_tag="VKQ27_05140"
     CDS             complement(10507..>11964)
                     /locus_tag="VKQ27_05140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLY88350.1"
                     /translation="VLVLSLVLWVKFDTGEPGFQFIEEIPWLPEYGVGYKLGVDGISV
                     LFVLLSTLLTPICILASWESIKRSVREYMLTFLILETMMVGMFCALDFVVFYVFFEGV
                     LVPMFLIIGIWGGPRRVYSAFKFFLYTFAGSVLMLLALLAMWFQAGTTDITVLMKTPM
                     PVGMQTWLFLAFFASFAVKVPMWPVHTWLPDAHVEAPTAGSVILAGVLLKMGAYGFLR
                     FSVPMLPQASAYFAPFIFTLSVVAVIYTSLVALAQEDMKKLIAYSSVAHMGVVTIGIF
                     TFNVQGIQGALFQMLSHGIVSAALFLVVGVVYDRLHTREIARYGGLADRMPLYALTFM
                     IFTMATSGLPGTSGFVGEFLVIVGSLQVNFWLALLGGMGMILGVAYMLYLYRRVIFGR
                     LTKEDLRAILDLSPREITVFVPLILLTFWMGIYPSSFTGFWDASVAQMVQRHTAALET
                     PVKAAAVVPQPSEVRATGDGRVRPSHDVRVAGVHL"
BASE COUNT         2483 a         4018 c         3508 g         1955 t
ORIGIN      
        1 atccgattga gctgccgtgg atcgacgcgc gccgcgaagt ccgccagccc ggtaaacggc
       61 gtatcgccgc gcgcctgcgt cagcgcctgc atcgccgaaa agcctacctt tttcactgcc
      121 gccagcgcgt agcggatagc cagcttgccg tcatcaatgc gctccacagt gaagtctgcg
      181 ccggagttgt tgatgtcggg cggcagaatg cggatgccgg aacgcgacgc ctcctcacga
      241 agggcggcaa gtcgatcagt attgcccagc gcgaggctca tgcaagcagc gatgaacacc
      301 tcggcatggt tcgccttcat ccacgccgta tgataggcga ccagcgcata ggctgccgcg
      361 tgggacttgt tgaagccgta gtcagcaaat ttcgccatga gatcgaagac ctcgatggcc
      421 ttcgcctttg cgatgccgtt cgctgtggca ccctgcacaa aaatctcgcg ctgcgcctcc
      481 atctccgagc ggatcttctt gcccatcgcg cgccgcagca gatccgccgc gccaaggctg
      541 tagcccgcca tcttctgcgc gatctgcatc acctgttcct gatagaccat gatgccatag
      601 gtctcttcca ggatatcgtg gatcagcgga tgcggcgcct cccatgattc tccgtgcttg
      661 cggcggcaat aatccgggat gttggccatc gggccgggcc gatacagcgc gccaagcgca
      721 atcaggtcat cgaacctgtc aggccgcatt tgacgcagcg cgtcgcgcat gccctgacct
      781 tcgaactgga acaccccgcc agcatcgccg cgctgcagca tctcgtaggt cttcggatcg
      841 tccagcggta ccttgtccag atcaacctcg atgcccaacc cgtgcagcat gcccaccgcg
      901 cgctgcagaa tggtcaacgt cgtcaaccca aggaagtcga acttcaccag ccccgcctgc
      961 tcgacgtatt tcatcgagta ctgggtaacc aggaagtcgg accgaggatc gcgatacaac
     1021 ggcaccagct caatcagcgg ccgatcgccg atcaccacgc ccgcagcgtg ggtggaggca
     1081 tgtcgataca gcccctcgat ctgcaacgcg atttccagca accgcgcgat accttcgtcc
     1141 tcatcgcgca tcatccgcag acgcggctcg ccggcgatgg cttcctgcaa gttcaccgga
     1201 tgcgccggat tgttcggaat caattcggcc accttgttca cctggccgaa cggcaaccca
     1261 agcacacgcc caacatcgcg caccgccgcg cgcgcctgca acttgccgaa agtgatgatc
     1321 tgcgccacac gatcgccgcc gtactcgcgc cgcacatagt cgatcaccgc atcgcgacct
     1381 tcctggcaga agtcgatgtc gaagtcaggc atcgacacgc gctcgggatt caggaagcgc
     1441 tcgaacagca ggttgaagcg gatcggatcg agatcggtga tggtcagtgc ccatgccgca
     1501 acggatcccg cacccgaccc gcggccagga ccgacaggaa tcccctgcgc cttggcccac
     1561 tggatgaagt cagcaacgat cagaaagtag ccggggaacc ccatcttgca gatgacatct
     1621 agctcgtagt caagccgttc gcgataacgg ccgcgcgtgc cttcgtccgc acccatcgca
     1681 tccatccggc gatcgagacc ttcgatcgcc atgacgcgca cggtctcctc ttccgtgttg
     1741 ccggcgcgaa ccttcggtga gacaggaagc agcggcttgc gcgtctccat catcaccgca
     1801 caacgctgcg caatggcaat cgtattgtcg caggcttccg gcagatccgc gaacagcatc
     1861 cgcatatcag gcgccggctt gaaccagtgg tcgggcgaga cgcggcgccg atcaggttcc
     1921 gaaagcagcc ttccctctgc gatcgccagc agagcatcat gcgcttcgta tttgtcgggc
     1981 gtcggaaaat agcagtcgtt ggttgcaacc agcgggatgc ctgccgcatc ggccaacgcg
     2041 agcagcccag gctccaccgc ccgttcagac tgcagattgt gacgatgcag ctccattgcg
     2101 acccgatccg gaaacccctc ggcgaagcgc gccaacaacc gctcggcctc gtgtttctgc
     2161 ccctcgccga gcaagcgcgc cagtggccct gtcgcaccac cggtcaacag gatcaggccg
     2221 gcggcatgtt ccgtaatgac atcgatcgat agctgtgccc tcaggcccgg ttccgtgtcc
     2281 aggaaccccc gcgatgacag ccgttgcaga ttgccaagac cagttgggtt ctgcgccagc
     2341 agcacgatcg gatccggcgg caggcgcgga ttgtcggcgc gagtcagttc gatctggcaa
     2401 ccgatgatcg gctggatgcc cttggcagca caggcctggc tgaactccag cgcaccgaac
     2461 agattggctg tatcggtgat cgccactgcc ggcatgttcg catcccgcgc cagggcagga
     2521 atcttgtcgg ccttgatggc gccctcgctg agcgaatagg cggaatgaac acgcagatgg
     2581 acgaaatcgg catgggacat agtgccaatg taatgcgtgg cccgattctg accaacgcca
     2641 tgcgcgcgcc taagccgccg gcagcatcac caacctgccg tcccgcaacg tcaccgtccg
     2701 atccatccgt gcggcgagct caggattatg tgtcgcgatc agcgccgcga gcttctctcg
     2761 ccgcaccatc atcagcaact catcgaacac caccgcggcc gtggcaacat ccaggttgcc
     2821 agtcggctca tccgccagca gcacacgtgg ttcgttcgcc agcgctcgtg caatcgccac
     2881 acgctgctgc tcgccgccag agagtttgcc cggcagatgg tcagctcggt cttgcaagcc
     2941 gaacgaggac agtagcgcca tcgcccgttg cgcagccgcc ctgcgtggcg tgcgagcgat
     3001 catctgcggc aggaccacgt tttccagcgc ggtgaattcg ccaagcaagt gatggaactg
     3061 ataaacgaag ccgatggtgt cgcggcggat cgccgtacgt tgcgcgtcgc tcagcgcacc
     3121 ggcatcgcgg ccatcgacaa gaaccgagcc gccgtccggc ttctccagca gccccgccag
     3181 atgcagcagc gtggacttgc cagcccccga cggtgccacc agcgcgacga tctcgcccgc
     3241 atgcagcacc atatcggcgc ccgtcagcac cggcagctca ccggcctcag tgcggtagat
     3301 ccgccgcacg tcacgcagca ccagcggatc actcatggcg cagcgcttct acagggtcgg
     3361 tgcgggcagc cttccagctc gggtacagcg tcgccagcag cgacagcaca agggcgagta
     3421 tgacgacgcg gatcacgtcg ccccaatcga tcgagtttgg cagcgcggta agcatgaaca
     3481 ccgtctggtc gaacacctgc ccgccggtga cagcctccac gaagtgctgg atcgccacga
     3541 tgttgcggca gaacacgatg ccgatgattg tgccgatgat cgtgccggac acgcccacaa
     3601 acgcaccgca catcaggaag atgcgcagca cagcgcccgc atcggcaccc aacgttcgca
     3661 gcaccgcgat gtcgcgcgtc ttgtccttca ccatcatgat cagcgaagag atcacgttga
     3721 acgcggccac cagcacgatc atgccgagga caatgaacat cgtatccttc tgcacctgca
     3781 gcacgctgat gatcgtgtca ttggcatggc gccagtcgcg catccacacg tggcggtcgc
     3841 cgatcgcctt ctgcagaggc ggcaccagcg catcgacgtg gtcggggtca gtgacgcgga
     3901 tctcgatact ggtgatgccg tcaggcttct ggaagaacac ctgtgcggcc gccagcggca
     3961 tgaacacaac gttgttgttg tagtcgtaaa ggcccgcgtc gaagattgcg acgaccttgt
     4021 aggccttgac gcgtggcacc gtgccgaatg cggtcgctgc accttgtggg gacagcactg
     4081 tcagcgagtc gccaatgcgc aactggtagg attgcgccag gccgatgccg acgatgagcg
     4141 catcgtcgcc ggtgaaggcg tccagcttgc ccgcaacgat gtggtcgctg accgggtgca
     4201 gcgcgcgcag atcctccagc gtcattccgc ggaccaggcc acccttggac ccaccccggt
     4261 cggtggtgag caacgcctga ccatccagga ccgggagtgc cgagacaatg ccaggaatgg
     4321 cgcggatctg cgtaagcaat ggcgggtaat tgtcgatctg gctgcccgca aaggcctcga
     4381 tcgaaatatg gccgttgatg cccaggatgc ggctttccaa ttcggtttgg aacccgctca
     4441 tcacgctggt cacgacgatc agcgtcgcca cgccgagcgc aatgccgatg agcgaaaaga
     4501 tggcgatgat cgacacgaac cgctcgccct tgcgcgcgcg cagatagcgg ccggcaaccg
     4561 cgcgttcgaa gggtccgaac atatcctagc cgatgcgcgc caacgcttcg tccggcgtga
     4621 cgtcactgcg ctcgccggtt gcgcggcgct tcagttccac cttgccggcg gccgcgccac
     4681 gcggaccgac gatgatctgc cagggatggc ccatcagatc ggcgtccgcg aatttggcac
     4741 ccgcgcgttc ctcgcgatcg tcgtacagtg ccgccgaacc gagcctacca tacagcgctt
     4801 cgcacatcgc atcgcacgcg ccatcgcccc gacgcaggtt gaggatcgcc gccttgaacg
     4861 gtgcaatgct gtcaggccag atgatgccgg cttcgtcatg gcttgcctcg atcaccgcgc
     4921 cgacaagccg cgagacacca acgccatagc tccccatctc tggaaacacc ttggagccat
     4981 cggcggcagt gactgttgcg ttcatggcct tggtgtattt ggtgccgaag tggaaaatgt
     5041 ggccaacctc gatgccgcgg ccttcacgct tgcgtgcggc tgacacctgg tcccacttcg
     5101 ccgggtcgtg cttctcatcg gtcgccgcat acatcgacgt catctgcttg aagaacgcct
     5161 ccaggtcctg cgcgttatag tcgaacttcg cgttgaccca gtcgatgtcc tcgaagccgg
     5221 tgtcgtaata cacctgactt tcgccggtgg acgccaggat gatgaactcg tggctcaggt
     5281 cgccaccaat cggtccgctc tccgccgcca tcggaatggc cttcagcccc atgcgctgga
     5341 acgtgcgcat atacgccagc atcatcgtgc gatagctgac caccgccccg gcatagtcca
     5401 tgtcgaagga gtaggcatcc ttcatcagga actcgcgccc ccgcatcacg ccaaaccgcg
     5461 gccgcacctc gtcgcggaat ttccactgta tatggtaaag tatctgcggc agctcgcgat
     5521 acgaatgcac cgactgacgg aacagatcgg tgatcacctc ctcattggtg gggccgtaca
     5581 gcatgtcgcg ctcgtgcctg tcccggatgc gcagcatttc cttgccgtaa tcgtcatagc
     5641 gcccgctttc gcgccagagt tcggcagact ggatggtggg catcagcact tcctgcgcgc
     5701 cggcggcatt ctgctcctcg cggacgatgc gttcgatgtt cttcagcacc cgataaccaa
     5761 gcggcagcca tgcgtagata ccggccgagg tctgccgcac caggccggca cgcagcatca
     5821 gccggtgcga ggcaatctgc gcctcggcag gcgtttcctt catggtggga atgaagctgc
     5881 gggacaggcg catgggcatt gcatccgaac tggttggcgg ccggcgaccc tagcgggacg
     5941 cgcagtgcgt cgccaccccg atgcgacaca cggttcgatt gtgcactgca caacgacaat
     6001 agtgaaataa agcatcccag gatagcaaaa ttaggaaata caacgtgact cgagttcgat
     6061 ctcgcgctac caaggcggcg gctggaggcg ccgtttgtgc ccggccaagt ttggggagga
     6121 aacgccgtca cacggcaggc tggaaggaca cataggcgcc ttcccctgac cgtggtgatg
     6181 gaagccatcc gcccccgccc gtcatgaccg gccttgatcc cctgccacgt catgggccgg
     6241 gccgccacgt catggccggg cttgacccgg ccatcgcctg tcagtcctgt ggcgcagcga
     6301 accagccgtg gcgaaagctg atgtaagggc tggtgatcag cagccacgca ccaaaccaca
     6361 cgacggcggc aaccagcgtg gtgataatga ccttccgcaa cagttgcggc ttctgcggcg
     6421 caccgcgcca gccggtcgcc tcgtccgcct cctcaacggg ccgcgtgccg atcggcagaa
     6481 cagcgaacaa tgccgtccac cagatcagca catagaggac gacaccggta aaccagctca
     6541 tacccgcaac agatgcacgt caacgagcgg gcgcttctgc aagcgccggc ccaacgcgcg
     6601 ccgcagggct gcccgcgccg catcattgag cgcgtcatca tcgcgtcgaa gcgactgcgg
     6661 cagatcatcg atcgcgtcag cgaattccgt gtgtatacga tccagttcgg catcatcgtt
     6721 gtcgaataat ccgggcgcac tgatccgcgg ggcgccgcgc agacggccat tggcatccac
     6781 cgcaaggctc gccaccacaa cgccgttgaa cagcatgcgc cgccgcgctg tcatcacccc
     6841 gccgccaatc ggggtaaggc ggctgccatc cagcaccagc ttgtcaaccg gcgcgctgtc
     6901 ggtcacctcg gcgcgctccg gggacaggct gagcacgtcg ccgtcctcca gcaggatcgg
     6961 ctgcgcacca gcgtcgcgag caagcgccgc atgcgccgac agatgccgcc actcaccatg
     7021 cgtcggcacc gagaagcgtg gcttcaccag tctatacagc cggcgcagtt cgtcgcgtgc
     7081 gggatgcccg gagacatgca ccatatgatc ggcatcagtc atcagctgca caccacgacg
     7141 gaccagattg tcctgcaccg tgacgatcgc acgttcattg cccgggatca tgcgcgacga
     7201 gaagatcacc gtatcgccct gccccaacgc cacgcgcgga tgcgtatcca tcgcgatgcg
     7261 cgccaatgca ctgcgaggtt cgccctggct gccggtcacc atcatcagga tgttatcatc
     7321 cggcacatca tccacatcgt cttcggtgag aaacggcggg agatcgcgca gatagccgca
     7381 ctcgcgcgcc gctgcgtcga tgttgcgcag tgaacggcca accagcgcca cgctacggcc
     7441 tgcgtcgtgt gccgcaagcg ccacggactc caaccgtgcc acgttggagg caaagcaggt
     7501 taccgcaacg cgccctctca gatcgcggag caacacggac agcgtctgcc gcacatctga
     7561 ctcgctaccg gaatgacctt ccaccattgc attggtggaa tcgcagatca tggccaacac
     7621 gccgcgcgca cccagcgcct cgaattcagc ttcgttggtc ggctcgccga tcaggggagc
     7681 aggatcaagc ttccagtcac cagtgtgcag cagcaggccg tgtggcgtgt cgatcaccag
     7741 cgcctgcgct tccggaatgg aatgcgtcac gcgcaggaac ttcaggcgga acggcggcag
     7801 atcgatcggt gcccccggcg ccaccacatg cagcttcacc tggttgagca gctgaacttc
     7861 gttcaacttt cggcgcagca ccgcggcggc gaacggcgtc gcatagaccg ggcagcgcag
     7921 ctgcggccac agccaggcga ccgcgccgag atgatcctca tgcgcatggg tgatcaccag
     7981 cccgaccagc ttatccgcgc ggtcggcgat gaaagccggg tcgggcatca tcacatcgac
     8041 ttccggatgc gccgcgccgc cgaaaccaat gccgcaatcc accgcgagcc atctgccatt
     8101 gcagcggtac aggttgaggt tcatgccgat ctcgcccgtc ccgcccagcg gcaggaaggc
     8161 aaggtcgctg ttatcaaaat ccattcgttg ctttcgttag tcctgttcga tcagacgcgc
     8221 tctgtcagcg cgtaatgtgg gcacggggtt gcgctcagcc aacagccgca gcccatccaa
     8281 tgtcaaatcc ggctcgatct gctcgatcac catggtgcct tcggccacca gggacgcgag
     8341 gccgccggtc gccaccacct tcatcgggcc gccgtattcg gccttgatcc gcgcgaccag
     8401 gccctcgacc atggcgacat agccccaata aatccccgac tgcatggcgg ggacggtctg
     8461 ccggccgatc accgcttgtg gccgggcgat gccgatacga ggaagctgcg cggctgcctg
     8521 atgcagcgcc tccagcgaca ggttgatgcc cggacagatc acgccgccga gataggcccc
     8581 atccttctcc acaacatcga atgtcgtggc ggtgccgaaa tctactatga ccagcggccc
     8641 accgaaccgc cgatgcgcgg ccagcgtatt gagcaggcgg tcggcgccaa cttccgcggc
     8701 gttcaccacg cgaatgtcga agccccaatc cagcgtagag cgcgcgatca acggctcgac
     8761 attgaaccag tcgcgacaaa tgcggcgcag gtgatacagc gccgcaggta caacggtgcc
     8821 gatgaccgca gaggagatgt cctcgcgctt caggccggag tgctcgagca gtgacagcag
     8881 ccagacgctg tactcgtcgg acgtgcgctg cgcatcggtg cggatgcgcc aggtgccgcg
     8941 ccatgcagcg ccatcgcgca ctgcgaacac gacgttggtg ttgccgcaat ctatgacgag
     9001 aagcatgcaa cctcgctccc ctcgtcattg cgagcacagc gaagcaatct cgatcgggat
     9061 tgctccactg cactcgctac gacagggatc atccgaacag caccttcgcc gccgcctgcg
     9121 cggcgccgac gaagggcgca gggaagacga tgaagaacgt ggtgaacaac ccggtcgccg
     9181 cggcgacaaa agacaccgac gcagggcgtg gatcgaatgc ctcttccgga gcgtcgaaat
     9241 acatcacctt gacgacgcgc aggtaatagt aggcccccac aacgctggtg agcacaccga
     9301 tgatggcgag cgtccacatg ccgccctgca cggcggcgag gaacacgtag agcttgccaa
     9361 aaaagcccga cagcggcggc acgccagcca tcgagaacat gaacaccgcc atggccagcg
     9421 ccagcgcagg gtcagtgcgg ccaaggccat tcaggtcgga gatcagctcc agcgagcggc
     9481 cccgccgctg catggcgatg atgcaggcaa aagtgccggc gctcatgaac acatagacaa
     9541 caagatagac aagcacgccg cggatacctt caggcgtgcc gaccgccagc ccgatcagcg
     9601 cataacccat gtgcccgatc gaggaatacg ccatcagtcg cttgatgttg gtctgtccga
     9661 tggccgcaag tgcccccagg atcatggaag cgatcgaaat cagcacgatc agctcttgcc
     9721 aggcgccgac cagatgcccg aacggtgtcg ccatcacccg cagcagcagc gcgaaggcgg
     9781 caacctttgg cgcggtagag aagaagacgg tgaccggcgt cggcgagcct tcgtaaacat
     9841 ctggcgtcca catgtggaac ggcaccgcgg agatcttgaa cgccagaccg accaggacga
     9901 acacaacccc gacgatcagg ccaggtgaag ctgcctctgg acgtgccagc gccctggcaa
     9961 gcgccgcgaa gtccatggtg ccggaaaagc cgtaaaccag cgaaatgccg tagagcaaca
    10021 gacccgatgc aagtgcgcca agcacgaagt attttagccc cgcttccgaa gaacgcagct
    10081 cgtcgcgcgc aaacgccgca agcacataga gcgccagcga ttgcagctcg agcccgagat
    10141 acagcgtcat catgttggac gctgacgtca tcaccatcat gccggtgacg gacagcacca
    10201 tcaggacagg gaattcgaag cgcgcgatat ggttcttgcg gttgtagtca agcgacagca
    10261 tcagcgacag cgcagcgccc gccaggatca gcagcttgac gaacgcagag aatgcatcgc
    10321 tgacgaactg gccgtggtag ccgagacccc ggtcgcccgt cagcacgagc aacgccgcca
    10381 gcaggaagcc gccaagcgcg aacatcgagg acagcaggaa gctgtcctct ttgcgcagaa
    10441 cgccgaagat cagcactgcc atcgcgacga tggacagcac gatttcagga agtgcgacgg
    10501 tccagttcat agatgcaccc ctgccacacg cacgtcatgg ctgggcctga cccggccatc
    10561 cccggtggca cgcacttccg aaggttgcgg cacaaccgcc gcggccttca ccggcgtctc
    10621 cagcgccgcc gtgtgccgct gcaccatctg agcgacgctc gcgtcccaga aaccggtgaa
    10681 gctcgacgga tagatgccca tccagaacgt gagcaggatc agcggcacga acaccgtgat
    10741 ctcccgcggc gagagatcga ggatcgcccg caggtcttcc tttgtcagcc gcccgaatat
    10801 cacccggcga tacagataca gcatgtaagc cacgcccagg atcatcccca tcccgcccaa
    10861 cagggcgagc cagaagttca cctgcagtga accgacgatc accaggaact ccccgacgaa
    10921 gccggacgtc cccggcaacc cgctcgtcgc catggtgaag atcatgaagg tcagcgcgta
    10981 aagcggcatc cggtcggcca gcccgccgta acgcgcgatc tcgcgcgtat ggagccggtc
    11041 atagacaacg cccaccacaa gaaacagcgc ggccgagacg atcccgtgcg acagcatctg
    11101 gaacagcgcg ccctggatgc cctgcacgtt gaaggtgaag atgccgatgg tcaccacgcc
    11161 catatgcgcc acggacgaat aggcgatcag cttcttcatg tcctcctgcg ccagggcgac
    11221 cagcgatgtg tagatcaccg ccaccaccga cagcgtgaat atgaacggtg cgaaatacgc
    11281 cgaagcctgc ggcagcatcg gcaccgagaa gcgcaggaac ccgtacgccc ccatcttcag
    11341 caacacgccc gccaggatga ccgagcccgc ggttggcgcc tcgacatgcg catccggcag
    11401 ccaggtgtgc accggccaca tcggcacctt caccgcgaac gacgcgaaga acgccaggaa
    11461 cagccacgtc tgcatcccga ccggcatcgg tgtcttcatc agcacggtga tgtcggtcgt
    11521 gccggcttgg aaccacatcg ccagcagtgc cagcagcatc agtaccgaac cggcgaacgt
    11581 gtacaggaag aacttgaatg ccgaatatac ccgccgcgga ccgccccata tgccgatgat
    11641 aaggaacatc ggcaccagca cgccctcgaa gaacacgtag aacaccacga agtccagcgc
    11701 gcagaacatg cccaccatca tcgtttccag gatgaggaag gtcagcatgt actcgcgcac
    11761 cgagcgcttg atcgattccc agctcgccag gatgcagatc ggtgtcagca gggtggaaag
    11821 cagcacgaac agcacggaaa ttccgtcaac gcccagcttg tagcccacgc cgtactcggg
    11881 aagccaaggg atttcctcga tgaactggaa gccaggctcg ccggtgtcga acttcaccca
    11941 cagcaccagc gacagcacca ggac
//
LOCUS       DATGEM010000165         9055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_865335, whole genome shotgun sequence.
ACCESSION   DATGEM010000165 DATGEM010000000
VERSION     DATGEM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865335"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VKQ27_05145"
     CDS             complement(<1..406)
                     /locus_tag="VKQ27_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88351.1"
                     /translation="MMHPALVIGAGVVGLSTALYLQRSGVQVVVLDPLGPAGGASFGN
                     AGMLSPDTSTPIAMPGMLRKIPGWLRDPLGPLAVRPSYFPRALPWLLQWVKAGRIDRV
                     LAISDAMRALHVQTIDCWRELLGDENYRDLIRP"
     gene            complement(427..1503)
                     /locus_tag="VKQ27_05150"
     CDS             complement(427..1503)
                     /locus_tag="VKQ27_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88352.1"
                     /translation="MSKLVLRNLGKSFGATAAVQDVDLTLQEGEFVSLLGPSGCGKTT
                     TLRMIAGFITPSNGTIEMNGAVLSAPNRVVAPEKRGMSMIFQSYAIWPNMTVAQNVAF
                     GLKLRKLPAAEQRARLDRILEVVRLGHLQDRYPAELSGGQQQRVALARAIVVQPEVLL
                     LDEPLSNLDANLREEMRAEIRRLHDAFRITTVYVTHDQSEAMVTSDRIVVMNHGRIEQ
                     VDDPVSLYTRPRTRFVAGFIGRTNFLDGAVNDDIIAFPGFTVRANGVAEGAQGDISFS
                     VRPQDIAIYRDAPKDANGGWWVEAVVAERAYLGDCWDYGVRPVASELRLKVSAPPQAV
                     YEAGDTVWLEIDPRRIARIPGDED"
     gene            complement(1500..3218)
                     /locus_tag="VKQ27_05155"
     CDS             complement(1500..3218)
                     /locus_tag="VKQ27_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLY88353.1"
                     /translation="MARHVQLAASARVRTLEPSLLLWIALAAVLIFLVVNPLFRLVQM
                     SLQDTDTGAFTFHNYLAAYGRPRYLVAMWNSLQLGFWVTVLCLLFAVPIAWAVSRTDM
                     PWKGAIRILVLAAFVTPPYLGSIAWILLAGPNSGWLNRVWMALTGAQEGLFNIYSFAG
                     LAFNIAIYSFPYLFIFTSAALDVVSSEMEDAANILGAGTLRTTLRVTLPMVLPAILGG
                     AIVTFLEAIALFGTPALIAIPARFNVVTTQLLQFFSEPIRAEVAAAYAVPLLLVTVTL
                     FSVQNVLLRRRGFVVLTGKGGERREMALGPWRWVMLGYALLLLSLAVFLPYAALAQAA
                     LSKAWGRGLSLDNLTLHNFYYLLFEHDTAARSVVNSFTYAAAAACIAIGLALAIAYIV
                     SRRLLPFARVLSFVAMAPFVVPGIVLAIAFYAAYAPPPLALYGTALILILAFATRFLP
                     IGYANADAAIRGVNPEMEEAVRILGGGRLLAIRRVLAPLMQRSLVGAWLLVFIPATRE
                     LSTAMFLYGPKTRTMSVMLMDMSEEGNFENLAALGFLLLASTLVIVALASILLGRDFM
                     LRRERA"
     gene            complement(3197..4228)
                     /locus_tag="VKQ27_05160"
     CDS             complement(3197..4228)
                     /locus_tag="VKQ27_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY88354.1"
                     /translation="MTRKITRRTALAALGATALVSQGARADEASLLEGARKEGSLTWY
                     IAQVDGETAEVMGRAFTKRYPGVQVSVIRTTGQVAYERLMQDLKNNAPQCDVFGTTDI
                     AHMPALMKRDELAQFVPDNASALAPAFKGLGQDGWYHPVSSTLMLIIYNTQRIKPDEA
                     PKAWTDLLDPKLKGRVAFGHPAFSGYVGCWTLEMKTVYGWQYFDKLAKNNPRIGRSGN
                     DPITLLNAGECNAGLGPLATTLLSASKGNPIAVQYPTDGALLCIGPSAVMAKAPHPNA
                     ARLFQNWLLSQEYAQVCADNFVTPVRADVTLKPGAKPLADVKILRQTTAEIAKGVPEV
                     IEQWRDTFS"
     gene            complement(4233..5924)
                     /locus_tag="VKQ27_05165"
     CDS             complement(4233..5924)
                     /locus_tag="VKQ27_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY88355.1"
                     /translation="MGGEAFKAARDFLFASRIDYRKAHDEFRWPPLENFNYALDWFDA
                     ELARGDNAKRLALKIVGDGGGSATFGELSERSNRIANGLRALGVKRGERILLMLGNVV
                     PLWELMLAAMKLGAVVIPATTLLTPNDLKDRFERGRVRHLIANAADAQKFDGLDPTVT
                     RIGLGDAPAGWHSYADLLSGDMRFVSEGVTRATDPMLLYFTSGTTSRPKLVEHSHQSY
                     PVGHLITMYWLGLEPGDMHLNISSPGWAKHAYSCLFAPWNAAATVFVANQPRFNARGM
                     LDAITESKVSTLCAPPTVWRMFVQEDMASWETSLREVCSAGEPLNPEIIEHVQNVWGL
                     TVREAYGQTETTVQIACFPGQMVKPGSMGLEAPGYRIRLLDADDQDVDEGEVCIDLDP
                     PPIGLMRGYQNDDGSFAPLGKTAYRTGDVATCDADGYYTYVGRADDVFKASDYRISLF
                     ELESALIEHPAVAEAAVVPAPDPIRMAVPKAYITLTASSKADRETALSIFQHLRTTLA
                     PFKRVRRLEFTELPKTISGKIRRVELRHEEEARARQGRREAAEFREEDFPELKVG"
     gene            complement(5939..6835)
                     /gene="mmsB"
                     /locus_tag="VKQ27_05170"
     CDS             complement(5939..6835)
                     /gene="mmsB"
                     /locus_tag="VKQ27_05170"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HLY88356.1"
                     /translation="MAVIGFIGLGNMGAPMAANLVKAGHRVTGYDISASALQALASAG
                     VHAAGSAAEAARGADVVITMLPAGEHVRDVWLHQGGLIDVVRDAKPLLIDCSTIDVDS
                     ARTVSEAASVAGLEMLDAPVSGGVGGATAATLTFMVGGSDTAFARGLPVLQAMGKNIV
                     LAGGPGAGQAAKICNNMMLAINMVGVSEGFLLARKLGLDWDKLFQIASTSSGQSWALS
                     NYCPAPGPVPAAPSNRDYAAGFMAALMLKDVKLSQAAADVVGSPTPLAKQAMSFYQAV
                     VDAGDGAKDFSVVFRWLAAQQR"
     gene            7007..8119
                     /locus_tag="VKQ27_05175"
     CDS             7007..8119
                     /locus_tag="VKQ27_05175"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLY88357.1"
                     /translation="MPSEYPLPSSDPASLGFAAKPLEHLDRLIKQHIEEGRYSGAQIA
                     LARHGQLALFRTYGDAKVEPTRLPATNDNLFLLFSQTKVLTSSAVWTLVEEGKLSFMD
                     KVSDHLPEFACRGKADITLHQVMTHQGGFPSNNVTKATWTDHARMRAEVCDFALDWTP
                     GSKLQYHGQAAHLVQAMVIEAVTGQDYRDVIRERVIEPLSLDNDIYVGVPTQIQSRCA
                     DTYAPEPRDNSPEFRAAGLPSGGGFATARGIAAFYQMLLEHGRLGNVRLFSPRLVAYV
                     TRNHTGERPDAQMSDIPMHRGLGPHVRGESDRIRGLGTLGSPQTFGHGGVGSSYSWAD
                     PDSGVSFTYLTNYVYPDPWHSARLDRVSNLVHAAID"
     gene            complement(8400..9005)
                     /locus_tag="VKQ27_05180"
                     /pseudo
     CDS             complement(8400..9005)
                     /locus_tag="VKQ27_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
BASE COUNT         1794 a         3048 c         2624 g         1589 t
ORIGIN      
        1 aggggcgaat gaggtcacgg tagttctcgt cgccgaggag ttcgcgccag cagtctatgg
       61 tctggacatg cagcgcccgc atcgcatcgg agatcgcgag tacgcgatcg atacgcccgg
      121 ccttcaccca ttgcaggagc catggcagag cacgcgggaa gtaggaaggg cggactgcga
      181 gtgggccgag cggatcacgc agccacccgg gaatcttgcg caacatcccc ggcatggcga
      241 tcggggtgga ggtgtccggg ctgagcatgc ccgcattgcc gaacgacgcg ccaccggcgg
      301 ggccaagtgg atcgagaacg acgacctgca caccggatcg ctgcaggtac agcgccgtag
      361 agagcccgac cacaccggcg ccaatcacca gggcaggatg catcataacc acatctgcat
      421 attcgatcag tcctcgtcac cgggtatccg tgcgatccgc cgcggatcaa tttccaacca
      481 cactgtatcg cccgcctcgt acaccgcctg cggtggagca ctcaccttca agcgcagctc
      541 ggaggccacg ggccgcaccc catagtccca gcaatcaccc agataggcgc gctcagcaac
      601 aacagcttca acccaccagc cgccattcgc gtccttgggc gcgtcgcggt aaatggcgat
      661 gtcctgtggt cgcaccgaga agctgatgtc tccttgagcc ccttccgcca caccgtttgc
      721 gcgcactgtg aaaccgggaa aggcgataat gtcatcgttc acggcgccat ccaggaagtt
      781 cgttcgcccg atgaatccag cgacgaaccg cgtgcgcggc cgggtgtaaa gcgagactgg
      841 atcgtccacc tgctcgatcc ggccatgatt catcaccaca atccgatccg acgtcaccat
      901 cgcctctgac tggtcgtgcg tcacatacac ggtcgtaata cggaacgcgt cgtgcagccg
      961 gcggatttcc gcgcgcatct cctcacgcag attcgcatcc aggtttgata gtggttcatc
     1021 cagcagcagc acctccggct gaaccacgat cgcccgtgcc aaggcaacgc gctgctgctg
     1081 gccgcctgac aactccgccg gatagcggtc ctgcaaatgc ccaagccgca cgacctcgag
     1141 gatccggtca agccgggcgc gctgctcggc ggctggcagc ttccgcaact tcagtccgaa
     1201 cgcgacgttc tgcgccaccg tcatgttcgg ccagatcgcg tagctctgga atatcatcga
     1261 catgccacgc ttctccggcg ccaccacacg gttcggcgcg gacagcacgg caccattcat
     1321 ctcgatcgta ccgttgctcg gcgtgatgaa ccccgcgatc atcctcagtg tcgtcgtctt
     1381 gccacagccc gaaggcccga gcagcgatac gaactcacct tcctgcagcg tcaggtcgac
     1441 atcctgtacc gccgccgtag caccaaaact cttgcccaga ttccgcagca ccagcttgct
     1501 catgcgcgct cccgacgcag catgaaatcg cgtccaagca ggatggaggc caatgcaacg
     1561 atgaccaggg tggaagccag cagcaggaac ccgagggccg ccaggttctc gaaattacct
     1621 tcctcgctca tgtccatcag catcaccgac atcgtgcggg tcttcggtcc gtacaggaac
     1681 atcgcggtag acagttcccg cgtggcggga atgaacacca gcagccaggc cccaactagg
     1741 ctgcgctgca tcaatggagc gagaacgcgc cggatcgcca gcaaccggcc gccaccgagg
     1801 atccgcacag cttcctccat ttcgggattc acgccacgga tcgccgcatc cgcgttggca
     1861 tatccaattg gcaggaagcg cgtcgcgaag gcgaggatca ggatcagcgc cgtgccgtac
     1921 aacgccaaag gcggcggcgc gtaggcggca tagaacgcga tcgccagcac aatacccggc
     1981 accacgaatg gtgccatcgc aacgaaactc agcacacggg caaacggcag cagccgccgg
     2041 ctgacaatat aagcgatcgc caatgccagt ccgatggcga tgcacgccgc ggccgccgca
     2101 taggtgaagc tattgacgac cgatcgcgcg gcggtgtcgt gctcgaacag cagataatag
     2161 aagttgtgca gagtgaggtt atcgaggctc agtccgcgtc cccaggcctt cgagagcgcc
     2221 gcttgtgcca gtgccgcata aggcaggaac accgccagcg acaacagcag cagcgcatag
     2281 ccaagcatca cccagcgcca cggaccaagc gccatctccc gccgctcacc acctttgcca
     2341 gtcaacacga cgaacccccg ccggcgaagc agcacgtttt gcacgctgaa caaggtcaca
     2401 gtgacaagca gcagcggcac cgcataagct gccgctacct cggcacgtat cggttcactg
     2461 aagaattgca gcaactgcgt ggtgacgaca ttgaagcgcg cggggatcgc aatcagggca
     2521 ggcgtgccaa acaacgcaat cgcctcaagg aaagtgacaa tggcgccgcc gaggatcgcc
     2581 ggcagcacca tcggcaacgt aacgcgcagc gttgtacgca gcgtccccgc acccaggata
     2641 ttggcggcat cctccatctc ggacgacacc acgtcgagtg ctgccgaggt gaagatgaag
     2701 agatacggaa acgaatagat ggcgatattg aaggccagcc cggcgaacga atagatgttg
     2761 aacaatccct cctgcgcgcc ggtcagcgcc atccacaccc ggttcagcca tcccgagttc
     2821 gggccagcca aaaggatcca cgcgatcgaa ccaagatacg gcggcgtgac gaatgcggcc
     2881 agcaccagga tacggatcgc gcctttccac ggcatgtccg tgcgcgagac tgcccatgcg
     2941 atcggcactg cgaacagcag gcacagcaca gtaacccaga acccgagctg caatgagttc
     3001 cacatcgcga cgaggtagcg aggccggccg taagcggcca gataattgtg gaaggtgaaa
     3061 gcgccggtgt cggtatcctg caggctcatc tgcacgagcc ggaacagggg gttgaccacc
     3121 agaaaaatca acaccgccgc cagcgcaatc cacaacagca gcgacggttc aagcgtgcgg
     3181 acgcgcgccg acgcagctag ctgaacgtgt cgcgccactg ctcgatcact tccggcacac
     3241 ctttggcaat ctcggccgtt gtctggcgca gaatcttgac gtcagccagt ggcttggccc
     3301 cgggcttcag cgtcacgtca gcccgtactg gcgtcacgaa gttatcggca cagacctgcg
     3361 cgtattcctg cgacaataac cagttctgga acagccgggc agcatttgga tgcggtgcct
     3421 tcgccatgac ggccgagggc ccgatgcaca acagagcacc gtccgtcgga tactgcaccg
     3481 cgatcggatt gcccttggaa gccgacagca atgtggtcgc aagcggtccc agccccgcat
     3541 tgcactcacc ggcattcagc aacgtgatcg ggtcattgcc ggagcggccg atgcgcggat
     3601 tgttctttgc cagcttgtcg aagtactgcc agccatacac cgtcttcatc tccagcgtcc
     3661 agcagccgac atagcccgag aacgcgggat ggccaaacgc aacccgtcct ttgagcttcg
     3721 gatcgagcag gtccgtccag gccttcggtg cctcgtccgg ttttatcctc tgcgtgttgt
     3781 agatgatcag catcagcgtg gagctgacag gatggtacca gccatcctgc ccgaggcctt
     3841 tgaatgccgg tgccagggcc gacgcgttgt caggcacgaa ttgagccagt tcgtcgcgtt
     3901 tcatcagcgc cggcatgtgt gcgatgtccg tggtgccgaa cacgtcgcat tgtggcgcgt
     3961 tgttcttcag atcctgcatc agccgttcgt aggcaacctg gcccgtcgta cgaatcaccg
     4021 agacctgaac gccgggatag cgcttggtga acgcgcgccc catcacctcg gcggtctcgc
     4081 catccacctg cgcgatatac caggtcaacg aaccttcctt gcgcgcgccc tcgagcaatg
     4141 acgcttcatc cgcccgcgcc ccctgcgaca ccagggcggt ggcaccgagc gccgccagcg
     4201 ccgtcctgcg cgtgatcttc cgtgtcatgt cgtcatccaa ccttcagttc tgggaaatcc
     4261 tcttcgcgaa actcagccgc ttcccgccgt ccttgcctgg cccgcgcctc ttcctcgtgc
     4321 cgcagctcaa cgcgccggat cttgccggag atggtctttg gcaattcggt gaattcgagg
     4381 cggcgcacgc gcttgaacgg cgccaacgtt gtgcgcaggt gctggaagat ggacagcgct
     4441 gtctcgcgat cggctttgct gctcgccgtc aacgtgatgt acgccttggg caccgccatc
     4501 cggatcggat cgggcgcggg caccacggcg gcctcggcca ccgctggatg ttcaatcagc
     4561 gcgctctcca gttcgaacag gctgatccga tagtccgacg ctttgaacac atcatccgca
     4621 cgacccacgt atgtgtaata cccgtccgca tcgcacgtcg ccacatcgcc ggtgcgatag
     4681 gctgtcttgc caagcggggc aaacgatccg tcatcatttt gatagccacg catcagccca
     4741 atcggcggtg ggtccagatc gatgcagacc tccccctcat ccacatcctg gtcatccgca
     4801 tcgagcaggc ggatgcgata ccccggtgct tccagcccca ttgatcccgg cttcaccatc
     4861 tggccgggga aacatgcgat ctgcaccgtc gtctcggtct ggccatacgc ctcccgcacc
     4921 gtcaatcccc agacgttctg cacatgctcg atgatctccg gattcagcgg ctcccccgcc
     4981 gaacaaactt cgcgtagcga cgtttcccag cttgccatgt cctcctggac gaacatccgc
     5041 caaaccgtcg gcggcgcgca cagtgttgac accttgcttt cggtgattgc atccagcatc
     5101 ccccgcgcat tgaagcgagg ctgattggca acgaacactg tcgccgcggc gttccagggc
     5161 gcgaataagc aactatacgc gtgcttcgcc cagccaggtg aggaaatgtt caggtgcatg
     5221 tcgcccggct cgaggccaag ccagtacatc gtgatcagat gcccgaccgg ataactctgg
     5281 tgactgtgct ccaccagctt cggccgtgat gtcgtgcccg aggtgaaata tagcagcata
     5341 ggatcagtcg cccgcgtcac gccctccgac acgaagcgca tatcgccgct cagaaggtcc
     5401 gcataactgt gccatcctgc cggcgcatca ccgagcccta tgcgtgtcac cgtcggatcc
     5461 agcccatcga atttttgcgc atccgccgca tttgctatca ggtggcggac ccgccctcgc
     5521 tcgaaccgat ccttcaggtc attcggcgtc aacaaagtgg tggccgggat caccaccgcg
     5581 ccaagcttca tcgccgccag catcaattcc catagcggca ccacgttgcc gagcatcagg
     5641 aggatgcgct cgccgcgctt cactccaagc gcacgcagcc cattggcgat gcggttagac
     5701 cgttccgaca actcgccaaa cgtcgcgctg cccccgccat caccaacgat cttcagcgcg
     5761 agtcgcttgg cgttatcacc ccgcgccagc tcggcatcga accagtccag cgcataattg
     5821 aaattttcca gcggcggcca acggaattca tcgtgcgcct tgcggtagtc gatgcggctg
     5881 gcgaacagga agtcgcgcgc cgccttgaat gcttcacccc ccacgatcgc ctccgtcatt
     5941 atcgttgttg tgctgccagc caacggaaca ccacggaaaa gtccttggcg ccatcgcccg
     6001 cgtcgaccac cgcctgatag aacgacatcg cctgcttcgc gaggggcgtc ggagagccaa
     6061 ccacgtccgc cgccgcctgc gacagcttca cgtccttcag catcaatgcc gccatgaagc
     6121 ccgccgcata gtcgcgattc gatggcgcgg ccggcactgg acctggcgcc gggcagtaat
     6181 tcgacagcgc ccaggattgc cccgacgagg tcgaggcaat ctggaacagc ttgtcccaat
     6241 ccaatcccag tttccgcgcc agcaggaagc cttccgacac acccaccatg ttgatggcga
     6301 gcatcatatt gttgcagatc ttcgccgcct gccctgcccc cgggccgccc gcaagcacga
     6361 tgttcttccc catggcctgc agcaccggca ggcctcgcgc gaatgcagtg tcactgccac
     6421 cgaccatgaa cgtcagcgtt gcggctgtcg caccgccgac accgccggat accggcgcat
     6481 ccagcatctc caggccggcg actgaggccg cttcactgac ggtccgggcg ctgtccacgt
     6541 cgatcgtcga gcaatcgatc agcagcggct tcgcatcccg gaccacatcg atcaggccgc
     6601 cctggtggag ccagacgtcg cgcacgtgct caccggccgg cagcatggtg atcacgacat
     6661 cggcgccccg cgcagcctcc gccgcgctgc ctgccgcatg cacgccagcg gacgccaacg
     6721 cctgcaacgc gctggcactg atgtcatagc cagtgactcg atggccagcc ttcacaaggt
     6781 tggctgccat tggcgcaccc atattgccaa gaccgataaa gccgatgact gccatgtgcg
     6841 ccgccgtccc tccctatcat tgatgattcc tgtggccgat gagacccgat cctccggggg
     6901 acggcaagcg cagggcaggc gggatgctgt cggtcatagc ggctgatggc tgggcaccgg
     6961 cgatcgaagc gggttagcat ctctgcggct tcaccggagg aacgtcatgc ccagcgaata
     7021 tccgttgccg tccagcgacc ccgccagcct cggcttcgcc gccaaaccgc tggagcatct
     7081 cgaccggctg atcaagcagc acatcgagga aggccgctat tccggcgcgc agattgcgct
     7141 tgcccgccat ggccagctcg cactgttccg cacctacggc gacgcgaagg ttgaacccac
     7201 cagattgcca gcgaccaacg acaatctctt cctgctgttc agccagacca aggtgctgac
     7261 cagctccgcc gtatggacgc tggtggagga agggaagctg tcgttcatgg acaaggtgtc
     7321 cgatcacctg ccggaatttg cctgccgcgg caaggccgac atcaccttgc atcaggtgat
     7381 gacacaccag ggcggtttcc cctccaacaa cgtgacgaag gccacctgga cggatcatgc
     7441 ccgcatgcgc gccgaggtct gcgacttcgc gctggactgg acgcccggat cgaagctgca
     7501 atatcacggt caggcagcgc atctggtgca ggcgatggtg atcgaggcgg tcaccggcca
     7561 ggattatcgc gatgtgatcc gcgagcgggt gatcgagccg ctgagccttg acaatgatat
     7621 atacgtcggc gtgccgacgc agatccagtc acgctgcgcg gatacgtatg cgccggaacc
     7681 gcgcgacaac tcaccggagt tccgcgccgc cggcctgccg agcggtggcg gcttcgccac
     7741 ggcacgcggc atcgcagcgt tctatcagat gctgctggag cacgggcgcc tcggcaatgt
     7801 gcggctgttc tcgccgcgcc tggttgccta cgtgacccgc aaccacactg gcgaacggcc
     7861 cgatgcgcag atgagcgaca tcccgatgca tcgcggcctg ggcccacatg tgcgaggtga
     7921 gagcgatcgc attcgcggcc ttggcacact tggttcgccg cagaccttcg gtcacggtgg
     7981 cgtggggtca tcatattcct gggcagaccc ggatagcggc gtgtcgttca cgtatctgac
     8041 gaactacgtg tatccggacc cgtggcattc cgcgcggctg gatcgcgtgt ccaacctggt
     8101 gcacgccgcg atcgactaat cgttggagtt ggcaggcttc ggcgccgcgg gtgcgccgag
     8161 ccgccagccc acttcgcttg cgaacgcctc gaagaagtcc agctcgtacg ccgctccgac
     8221 gactgccgca caagctggtc gatgcggtgc gcgtcgtgtc ctaccaggat gcgccagcgc
     8281 tccgccttca ctccgtccag gatgatggtt gccgtagttg gagcatccac cagaaagtgc
     8341 cgtgggcgct ccaggaccat ggtctggata tcgccatccg acatgtgtgt gacgtctatt
     8401 cagcctgccg cagcgcgttc gcgagcctgg gcaatctcgg cagcggacag catgtccgta
     8461 tcgtttccac tgcgaatcct tcgggaattc gcgacgatgg atgtgccgat atgacccggc
     8521 atgaccacgg agcactttat gtgcggcgca tgcagacgca gatcggtgat cagcgcctcg
     8581 gtgaatccct tcacagcaaa ttttgcggcg caatacgcca tgtgcgatac gcgcggtccc
     8641 accgacgccc agaacccgtt gacgctgctg gtgttgataa tgtgtgcggc atccgcctgc
     8701 agcatcagcg gcagaaaggt ccgtgtgcag agatacacgc caccccagca gatattgaag
     8761 gtgcgcttct attcttcccg gctgttggcg atcatgctgc cgccgccgct gatgccagcg
     8821 ttattgagca acaggtggat ctggtcggtc tgctgccgct cctccacctc gtcacggaaa
     8881 cgctccacct gggcggggtc cgagacgtca gccagatgcg aggtaaggcg caggccccgc
     8941 ggcaggcgct gcaattcgca gagccgctgc gtctccgaca tcgcgcccgc tgacgcatcg
     9001 cacatgtcaa cgttgcagcc ctcggcgacc agttgacgaa ccagctcggc ccatg
//
LOCUS       DATGEM010000166         4992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_525572, whole genome shotgun sequence.
ACCESSION   DATGEM010000166 DATGEM010000000
VERSION     DATGEM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525572"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="benC"
                     /locus_tag="VKQ27_05185"
     CDS             complement(<1..935)
                     /gene="benC"
                     /locus_tag="VKQ27_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase electron transfer
                     component BenC"
                     /protein_id="HLY88358.1"
                     /translation="MTYRIALNFEDGATRFIDCKEGEKVLDAAYRNKVNLPMDCSDGV
                     CATCKCRCEQGEYDLGNDYIEDALTEDEAREGLVLTCQMVPSSDCVIAVPVPSTACKV
                     TPAEHGGEVASVELVSDSTIILKLKLDAPDALDFLPGQYVSITVPGTGEHRSYSFSSK
                     PGAEEASFLIRNVPNGLMSGWLADQAQPGARMTFVGPQGSFFLRGVERPVVMLAGGTG
                     LAPLLSMLEVLADQGTDQPTHLIYGATKDDDLVEIDRLEALADRLPTFTWVSCVADPE
                     SAHPRKGYVTAHLKDEHLNAGDCDVYLCGPPPMVEA"
     gene            complement(967..1473)
                     /gene="benB"
                     /locus_tag="VKQ27_05190"
     CDS             complement(967..1473)
                     /gene="benB"
                     /locus_tag="VKQ27_05190"
                     /EC_number="1.14.12.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate 1,2-dioxygenase small subunit"
                     /protein_id="HLY88359.1"
                     /translation="MTLALQSSLGIEQVCAFLYREARLLDDRQWDEWLAFYSPSVVFW
                     MPAWDDDDQLVEDPQAHISLIYYPNRGGLEDRIFRIKTERSGASTPEPRTSHTISNVE
                     ILEERDGEIDLRFNWHTLSHRYKKTDSFFGTSFYTLDTSSGEPVIVAKKVVLKSDYIH
                     QVIDIYHI"
     gene            complement(1470..2840)
                     /locus_tag="VKQ27_05195"
     CDS             complement(1470..2840)
                     /locus_tag="VKQ27_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY88360.1"
                     /translation="MATILERLDNAVIEDEERGVYRCRRDIFTDVELFELEMKHIFEG
                     NWVFLAHESQVTENSDYFTTWIGRQPIVVTRDKTGELHALVNACAHRGAMLCRRKHGN
                     KGSFTCPFHGWTFNNAGKLLKVKDGKTGDYPETFNTDGSHDLKRVPRFENYRGFLFGS
                     LNPDVVSLEEHLGGAKAVIDQIVDQAPDGIEVLRGNSSYIYDGNWKLQMENGADGYHV
                     SSVHWNYSATMGHRSYEAEGTRAVDPDGWSKSVGGVYSFEHGHILLWTKLLNPEVRPI
                     FAHREELESRLGKDCAGFIIGQTRNLGLYPNVYLMDQFSTQIRVVRPISVGQTEVTIY
                     CFAPKGENASERATRIRQYEDFFNVSGMGTPDDLEEFRACQTAYAGAGDLWNDLSRGA
                     TRWIGGPDANASAIGLKPLLSGERSEDEGLFVRQHEYWAETLRSAILRDGGDQAMAHS
                     EMGVAA"
     gene            complement(2863..3789)
                     /gene="catA"
                     /locus_tag="VKQ27_05200"
     CDS             complement(2863..3789)
                     /gene="catA"
                     /locus_tag="VKQ27_05200"
                     /EC_number="1.13.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019615 - catechol catabolic process,
                     ortho-cleavage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HLY88361.1"
                     /translation="MTQLTESPEIEAFLDKIAGINNPGAAGSQRTREILRKIVSDLFD
                     TIDRFDVSEDEFWHALNFLAAGAHEFGLWAAGLGFERFLDIRTDAKDRAAGVPEGTPR
                     TIEGPLYVAGAPIVEPGARIDDGSDMGETLVMHGQVRDTEGKPVAYAIVDVWHANTKG
                     NYSYFDKSQSDYNMRRRIKADAEGLYAFRSIVPHGYGVPEGGSTDQLLKAIGRHGQRP
                     AHIHFFVTAHGYRQLTTQINIDGDPYLHDDFAYATRDDLIPAVVRKTDPADIQAAGVD
                     APYADIVFDFTLAPAATAEDEAFSSRSRALAG"
     gene            complement(3846..4136)
                     /gene="catC"
                     /locus_tag="VKQ27_05205"
     CDS             complement(3846..4136)
                     /gene="catC"
                     /locus_tag="VKQ27_05205"
                     /EC_number="5.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016159 - muconolactone delta-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase"
                     /protein_id="HLY88362.1"
                     /translation="MLIMVEMDVRIPLGFDKGEAERLKAAEKARFQDLQRSGKWRHIW
                     RVVGRYANVSIFDVESNGELHDILISLPLFPFMEMKVTPLCRHPSSIHDDDR"
     gene            complement(4140..>4992)
                     /locus_tag="VKQ27_05210"
     CDS             complement(4140..>4992)
                     /locus_tag="VKQ27_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018849 - muconate cycloisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018850 - chloromuconate cycloisomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="muconate/chloromuconate family cycloisomerase"
                     /protein_id="HLY88363.1"
                     /translation="CAVETALLDASGKRLGVPVSELIGGGRVRDRLPVAWTLASGNTT
                     RDIEEAEAMIEARRHSIFKLKIGKRSVAEDVAHVGAIKRGLGTRGSVRVDVNQGWDEA
                     QADLGMAMLADVGCDLVEQPVARDALPAMTRLSARHAIPLMADEALHGPRDAFAIAAV
                     AGARVFAVKIAQSGGLHPAHDVACIAAAAGIGLYGGTMLEAGIGTAASAHLFATFPRL
                     AWGTELFGPLLLTEEILAEPLDYADFSLGVPTGPGLGIALDEDKIAHFRRDRTAPTLH
                     VVSAAGA"
BASE COUNT          906 a         1590 c         1563 g          933 t
ORIGIN      
        1 gcctcgacca tcggcggcgg gccgcagaga tacacgtcgc aatcgcccgc gttcagatgc
       61 tcgtccttca ggtgcgccgt gacataaccc ttgcgcggat gcgcgctctc gggatcggcg
      121 acgcaactta cccaggtaaa ggtcggcaga cgatcggcga gcgcttcgag ccggtcgatc
      181 tcgacgagat cgtcgtcctt cgtcgcacca tagatcaggt gagtgggctg atccgtaccc
      241 tgatcggcaa gcacctcgag catcgacagc aggggcgcaa gccccgtccc gccggccagc
      301 atgacgaccg gccgctcgac gccgcgcaga aagaagctgc cctgcgggcc gacaaacgtc
      361 atccgggcac cgggctgcgc ctggtcggcc agccagccgc tcatgagccc attgggcacg
      421 ttacggatca ggaagctcgc ctcctccgcg cccggcttcg agctgaagga ataggagcgg
      481 tgctcacccg tgcccggaac cgtgatgctc acatactggc cgggcaggaa atcgagcgcg
      541 tcgggcgcat caagcttgag cttgaggatg atcgtgctgt cggaaacgag ctcgaccgag
      601 gccacctcgc cgccatgttc ggccggcgtc accttgcagg cggtggacgg cacgggcacc
      661 gcgatcacgc aatccgacga aggcaccatc tggcaggtga gcaccagccc ttccctggcc
      721 tcgtcctcgg tcagcgcgtc ttcgatatag tcgttgccga ggtcatattc gccctgttcg
      781 cagcggcatt tgcaggtggc gcaaacgccg tcggaacaat ccatcggcag attgaccttg
      841 ttgcgatagg ccgcatccag aaccttctcg ccctccttgc aatcaatgaa gcgagtggcg
      901 ccgtcctcga aattgagcgc gatgcgatat gtcattctat cctctccggc cacatcggcg
      961 acgacattag atgtgataga tgtcgatcac ctggtggatg taatcgctct tcagcacgac
     1021 cttcttggcg acgatcacag gctcgccgct gcttgtgtcc agcgtgtaga aggaagttcc
     1081 gaagaagctg tcggtcttct tgtagcggtg gctgagcgtg tgccagttga agcgtaggtc
     1141 gatctcgccg tcgcgctcct ccaatatctc gacgttcgag atggtgtggc tggtccgcgg
     1201 ctccggagtg ctcgcgcccg agcgctcggt cttgatacgg aagatccggt cttccaggcc
     1261 tccgcggttg ggataataga tcagcgagat atgcgcttga ggatcttcga ccagctggtc
     1321 gtcatcgtcc cacgccggca tccagaagac cacgctgggc gaatagaaag cgagccactc
     1381 gtcccactgg cggtcgtcca gcaagcgcgc ttcgcgatag aggaaggcgc agacctgctc
     1441 gatgcccagg ctcgactgaa gtgcgagggt catgccgcaa ctcccatctc ggaatgggcc
     1501 atcgcctggt cgccgccatc ccgcaatatg gccgaacgaa gcgtctcggc ccaatattcg
     1561 tgctggcgca cgaagaggcc ctcatcctca ctgcgctctc cgctcaagag cggcttcagg
     1621 ccgatcgcgc tcgcattcgc gtccgggccg ccgatccagc gcgtcgcacc acggctgagg
     1681 tcgttccaca gatcgccggc gccggcatag gcggtctggc aggcgcggaa ctcttcgagg
     1741 tcgtccggcg tgcccatgcc gctgacgttg aagaaatcct catactggcg gatgcgggtc
     1801 gcgcgctcgc tggcattctc acccttcggc gcgaaacaat agatggtcac ctcagtctga
     1861 ccgacactga tcggccgcac gacgcggatc tgcgtcgaga actgatccat cagataaaca
     1921 ttcggataga ggccaagatt acgggtctgg ccgatgatga agccggcgca gtccttgccg
     1981 aggcgggatt ccagctcctc acgatgcgcg aagatggggc ggacctccgg attgagaagc
     2041 ttggtccaga gcaggatgtg cccgtgctcg aaactgtaga cgccgccgac gctcttcgac
     2101 cagccatccg gatcaaccgc tcgcgttcct tcggcctcgt agctgcggtg ccccatcgtg
     2161 gccgaatagt tccagtgaac ggagctgaca tgatagccgt cggcgccatt ctccatctgg
     2221 agcttccagt tgccgtcata gatgtaggag gagttgccac gcagcacctc gatgccgtcg
     2281 ggagcctgat cgacgatctg gtcgatgacg gccttggcgc ctcccagatg ctcctccagc
     2341 gaaaccacgt ccgggttgag gctgccgaac aggaagccgc gataattctc gaagcgcggc
     2401 acgcgcttca gatcatgcga accgtccgtg ttgaacgtct ccggataatc gcccgtcttg
     2461 ccgtccttca ccttcaagag cttacctgca ttgttgaagg tccagccgtg gaagggacag
     2521 gtgaagctgc ccttgttgcc atgcttgcgc cggcagagca tcgcgccgcg atgcgcgcag
     2581 gcattgacca gcgcgtgaag ctctccggtc ttgtcgcgcg tcaccacgat tggctggcgg
     2641 ccaatccagg tggtgaaata atcgctgttc tcagtcacct ggctctcatg cgccaggaac
     2701 acccagttgc cctcgaagat gtgcttcatc tcgagctcaa acagctcgac gtcggtgaag
     2761 atgtcccggc ggcagcgata gacgccgcgc tcctcgtctt cgatcaccgc gttgtcgagg
     2821 cgctcaagaa tggtggccat tgtttctctc ctcgtcctgc gctcagccgg ccagagcccg
     2881 cgagcggctg gagaatgcct cgtcctcggc cgtggcggcg ggggcgagcg tgaagtcgaa
     2941 gacaatatcc gcataaggcg catcgacgcc tgccgcctgg atgtccgccg gatccgtctt
     3001 gcggacgacg gcggggatca ggtcgtcgcg ggtcgcgtag gcgaagtcgt catgcagata
     3061 gggatcgcca tcgatgttga tctgcgtggt gagctggcga tagccgtgcg cggtcacgaa
     3121 gaagtggatg tgcgccgggc gctggccatg ccggccgatc gccttcagca gctggtcggt
     3181 cgaaccgcct tcggggacgc catagccatg cgggactatg ctgcggaacg catagagacc
     3241 ttcggcatcg gccttgatgc ggcggcgcat gttgtagtcg ctctggctct tgtcgaagta
     3301 gctgtaattg cccttcgtgt tggcgtgcca gacgtcaacg atggcatatg cgaccggttt
     3361 cccttcggta tcgcgcacct ggccatgcat gacgagcgtc tcgcccatat cgctgccatc
     3421 gtcgatacgc gcgcccggtt cgacgatcgg tgcgccggcc acatagagcg gcccctcgat
     3481 ggtgcggggc gttccttcgg gcacgccggc tgcgcgatcc ttggcgtccg tgcggatgtc
     3541 gaggaagcgc tcgaaaccga ggccggccgc ccagaggccg aattcgtgcg caccggcggc
     3601 gaggaagttg agcgcatgcc agaactcgtc ttccgagaca tcaaagcgat cgatcgtatc
     3661 gaacagatcg gagactatct tgcgcaggat ctccctggtg cgctggctgc ccgccgcgcc
     3721 cggattgttg atgccggcga tcttgtcgag gaaggcctcg atctcggggg actcggtgag
     3781 ctgggtcatg cgatttcctc tcaagcggtt tcgctctgtt cttctcggac caggcgacct
     3841 gaaggtcagc gatcgtcgtc gtggatcgag gagggatggc ggcaaagcgg cgtcaccttc
     3901 atctccatga aggggaagag cggcaggctg atcagaatgt catggagttc gccgttactc
     3961 tcgacgtcga agatactgac gttcgcgtag cgaccgacga cgcgccagat atggcgccat
     4021 tttccagacc gctgcaggtc ctggaaacgg gccttctccg ccgccttcag gcgttccgcc
     4081 tcgcccttgt cgaagcccag cgggatgcgg acatccatct cgaccatgat gagcatcgtt
     4141 caggctcccg ccgccgaaac gacatgaaga gtaggtgccg tacggtcacg gcggaaatgg
     4201 gcgatcttgt cttcatcgag cgcgatgccg aggccggggc ctgtgggcac accgagcgag
     4261 aagtcggcat aatcgagcgg ctcggccaaa atctcctcgg tgagcaatag ggggccaaag
     4321 agttccgtac cccacgccag cctcgggaat gtcgcaaaca gatgcgcgga tgctgcggtg
     4381 ccaatgcccg cttccagcat cgtgccgcca taaaggccga tgccggcggc cgcagcgatg
     4441 caggcgacat catgcgccgg gtgcaggccg cccgactgag cgatcttcac cgcaaaaaca
     4501 cgcgcaccgg cgacagcggc tatggcgaag gcatcacggg ggccgtgcag cgcctcgtcc
     4561 gccatcagcg ggattgcgtg ccgcgccgac agccgcgtca tagcgggtaa ggcgtcgcga
     4621 gcgaccggct gctcgaccag gtcgcagccg acatcggcca gcatcgccat gccaagatcc
     4681 gcctgcgcct cgtcccagcc ctggttcaca tccacgcgta cgctgccgcg tgtcccaagc
     4741 ccgcgcttga tcgcgccgac atgggcgaca tcttctgcca cgctgcgctt gccgatcttg
     4801 agcttgaaga tcgaatgccg ccgcgcctcg atcatcgcct cggcttcctc gatgtcgcgt
     4861 gtcgtgttgc cgctggctag cgtccacgcg accggaagcc gatcgcgaac gcgcccacca
     4921 ccgatcagtt cggacacagg cacgccgagg cgcttgccgg atgcgtcaag aagggccgtc
     4981 tccaccgcgc ac
//
LOCUS       DATGEM010000167         8024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_67824, whole genome shotgun sequence.
ACCESSION   DATGEM010000167 DATGEM010000000
VERSION     DATGEM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67824"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..2044
                     /gene="mutL"
                     /locus_tag="VKQ27_05215"
     CDS             311..2044
                     /gene="mutL"
                     /locus_tag="VKQ27_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLY88364.1"
                     /translation="MMATIRILPPHTVNRIAAGQVIERPAAVVKELVENALDAGASRI
                     AVALNGGGIDRIEVADDGCGMDAAGLALAVLRHATSKLADEDLVRIATLGFRGEALPS
                     IGAAARLAIVSRPREAAHASEISVEGGAVSEVAPAAGAVGTRVVVRDLFFATPARRKF
                     LKHPRTEADHAEAAVRRLALAAPEVAFRLESDGRVVFDLPPQERRERVGALFGRDAAE
                     AMVEVNGERGVLRIAGFACSPAVTRASNAMQALTVNGRPVADPVLRTSVRVAYRDVIA
                     VGRHPVVALWLDLPPEELDVNVHPAKTELRFRDVGAVRALVIGSVGRALAGGAGTALP
                     APRPSLHLVHSRPAAVPYPHVPLPGMAETQLAFGAGPAARVFPVAEPTAEHPLGAPVA
                     QVLDTYVIAVASDGSLVLVDQHAAHERLTHEALREQMLDGGVRTQPLLLPAVVDLPVA
                     DVGRLLGRAEELGKLGLEIEAFGPGAVMVRALPAALGSPEPGPLLRDLADELGEMEEA
                     TSLHARLDAVIARLACHGSVRAGRRLNAAEMDALLRQMEATPRAATCSHGRPTFLKLT
                     KAEIEKLFGRR"
     gene            complement(2126..3136)
                     /locus_tag="VKQ27_05220"
     CDS             complement(2126..3136)
                     /locus_tag="VKQ27_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY88365.1"
                     /translation="MQPTRRRILGGTAAVLASPFLARTARAAEYTWKFAHTAPQSFPF
                     HIRLAAAAAQINKESNGRMDFQVFPDGQLGGDNDLLSQARQGAIEFCQPTGQIFSSIL
                     PVTAISALGFAWSDYAKVWPAMDGDLGAYIRKQIAAKSGLVAMDRMWDLGFRQITSST
                     KPIRNAADLVGFKIRTPVAPSLVTLFQTLKAAPLALQFPELYSALQTHIADGQENPLT
                     LIKVAKFYEVQKYCSLTNHVWDGHWLCCNAAAWNGLPDDLKTIVARNLNNAALLERAD
                     IATNDKSVQGDLEKSGMVFNTAETQSFRDGLKEGGFYKLWHDKLGDEPWALLEKYTGK
                     LG"
     gene            complement(3270..4763)
                     /locus_tag="VKQ27_05225"
     CDS             complement(3270..4763)
                     /locus_tag="VKQ27_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLY88366.1"
                     /translation="MANQLGKIEHIVQLMLENRSFDQMLGFLYSDSNNRSPTGDAFDG
                     LTGDEWNPDDLGRQIKVYKIKATDPNPYLMPGADPGEGFYNTNYQLYSTDDPHPGAVP
                     DNKGFVINFKAAIASDLAKHYKDTLPGTQASQIMGMYTPELLPIMSGLAKGYAVCDRW
                     FASAPTMTMPNRAFAAAGTSQGHLDDHTKIFTCPSIFGRLSDKNIDWAIYGYNRDPLT
                     RHDFPDTQNADENHFGHFRDFQERAAAGTLPAYTFLEPSWDATGNSQHPNYDVAKGEQ
                     LIHDVYYALRNGRAWASTLLLITYDEHGGNYDHVAPPTDALPPDDGTVGEFGFDFKRF
                     GVRTPAVLVSPLIKAGTVYRGSGHIDHTSVLRTIHDRWGTTSLTARDKAAASLADALT
                     LAQARTDDPIKGVAPPVSGEHHPNSSQPSKIDKLHAARIAALPLRNEQGHYEEADASS
                     AQSASSADVGNFIRDRSAAWQQHVQRQKQRRGRTSHGHTGSENHRRH"
     gene            complement(5070..6035)
                     /locus_tag="VKQ27_05230"
     CDS             complement(5070..6035)
                     /locus_tag="VKQ27_05230"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HLY88367.1"
                     /translation="MAHLPTPLEPMKRLTEHLGGPRLWVKREDATGIGFGGNKLRKLD
                     FVLHDAVSNGADTIVSGGVVQSNSQRQVAAAAAKLGLACHLAVYHGRLKAPTPEYTTS
                     GNAFLNRLFGAHLHDVPWTGDRNEAIRAIVNNLEAKGHRPYFVPYGVSNALGAIAYAT
                     TIAEIASQASAQGFHPTAIVHCTGSAGTQAGLIVGASIAMPNTRIVGIDIDAEPDRVR
                     ADVVRFAREAANLLNVPFDESAVEVVAGHAGPAYGIPHAATIEAIRLAGRLEAMPLDP
                     VYSGKGLAGLIALIREGRWHKDDDVVFINTGGAPALFAYQSALAI"
     gene            6249..6614
                     /locus_tag="VKQ27_05235"
     CDS             6249..6614
                     /locus_tag="VKQ27_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY88368.1"
                     /translation="MRDHAKDHETLSDLNRNYVRSVDEADTSWFDANLANDFLNTNPD
                     GTLIDRSAFIAQIGRGSLVKDIREHDVVIRIFGDFAIIHARTAYLKPDGTQGGGRYTD
                     DWLFRDGRWQCVSAHVARL"
     gene            complement(6652..>8024)
                     /locus_tag="VKQ27_05240"
     CDS             complement(6652..>8024)
                     /locus_tag="VKQ27_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HLY88369.1"
                     /translation="LTQLRPATILALITAAVLIAAFVFLHPTLLTLGRLNLLIFFLGV
                     VTACVFAGIPIAVSFGLATLGYLAFATHVPLAVLVARMDTGMSHPVLLSVPLFVFLGL
                     LIDMTGMAQVMVRFLANLLGHVRGGLSFVLIGAMYLVSGISGSKAADMAAVAPVLFPE
                     MEQRGATRGDLVALLAATGAQTETIPPSLILITIGSVTSISIAALFTGGLLPGLVVGL
                     ILCAMVGWRARREDRMLPPRASSREIGRSFLIAIPALVLPFVIRGAVVGGIATATEVS
                     TIGIVYSVLAGVLIYRRFDLARLLPMLVQTAALSGAILLIVGAATSMAWAITSSGFSR
                     VVAGWIAEMPGGAFTFMAISILLFVVLGSVLEGIPVIVLFGPLLFPLAKQVGINEVHY
                     AMVAILAMGVGLFAPPFGVGYYVACAIGRCNPDDGMRHIWRYLAALIVGLIIVAAVPW
                     ISIGFL"
BASE COUNT         1339 a         2275 c         2978 g         1432 t
ORIGIN      
        1 gaggcggtgc ttaccgatcc ggagagcggg ctgattgctg cgggggcgga tcctcggcgt
       61 ccggcttacg cgattgctat ttgagccata ccgggcaggg tttcgccggg ttggtgcggt
      121 tgaatgatta acgcaaagca cgcaaaggac cgcagagaac gcaaagaagg gtttatttgg
      181 gcgctttgcg ccgaggctct gaaggtcggt gcggagcacc ataagtacct ctgcgttctt
      241 tgcgttcctt tgcgccctct gcgttgaaac ctttcttcat tcacatgagc accggcggca
      301 ttagtattgg atgatggcca cgattcgcat cctgccgccg cataccgtca atcgcatcgc
      361 tgcgggccag gtgatcgagc ggccggccgc ggtggtgaag gaactggtgg agaatgcgct
      421 cgacgccggg gcctcgcgga tcgcggtggc gctcaacggt ggcggcatcg accgcatcga
      481 ggttgccgat gatgggtgcg gcatggatgc cgctgggctc gcgctggcgg tgctgcggca
      541 cgccacgtcc aagctggccg acgaggacct ggtgcggatc gcgacactcg ggttccgggg
      601 ggaggcgctg ccctcgatcg gggcggcggc gcggctggcc atcgtgtcac ggccgcgcga
      661 ggcggcgcat gcttcggaga tctcggtcga gggcggggcg gtgtcggagg tggcgccagc
      721 ggcaggcgct gttggcaccc gggtggtggt gcgcgacctg ttcttcgcca cgccggcgcg
      781 gcggaagttc ctgaagcacc cgcggaccga ggcggaccat gccgaggcgg cggtgcggcg
      841 actggcgctg gcggcgccgg aggtggcgtt ccggctggag agcgacgggc gggtggtgtt
      901 cgacctgccg ccgcaggaac ggcgggagcg ggtcggggcg ctgttcgggc gcgatgcagc
      961 cgaggcgatg gtggaggtga acggcgaacg cggcgtgctg cggatcgcgg ggtttgcctg
     1021 ctcgccggca gtgactcggg cttccaatgc catgcaggcg ctgacagtga acgggcggcc
     1081 ggtggccgat ccggtgctgc ggacttcggt gcgggtggcg tatcgcgacg tgatcgcggt
     1141 ggggcggcat ccggtggtgg cgctgtggct cgatctgccg ccggaggagc tggacgtgaa
     1201 tgtgcacccg gcgaagaccg agctgcggtt ccgcgatgtg ggggcggtgc gggccctggt
     1261 gatcggcagc gtggggcggg cgctggcggg cggggctggc acagcgctgc cggcacctcg
     1321 gccttcactg catctggtgc attcgcggcc ggcagcggtg ccgtatccac atgtgccact
     1381 accggggatg gcggagacgc agctggcgtt cggggcgggg ccggctgcgc gggtcttccc
     1441 ggtggcggag ccgacggccg agcatccgct gggggcgccg gtggcgcagg tgctggatac
     1501 ctatgtcatt gcggtcgcgt ccgacgggtc gctcgtgctg gtggatcagc acgcggcgca
     1561 tgagcggctg acgcatgagg cgctgcgcga gcagatgctg gatggaggcg tgcggacgca
     1621 gccgctgctg ctgccggcgg tggtcgattt gccggtggcc gatgtggggc ggctgctggg
     1681 gcgggccgag gaactgggga agctcgggct ggagatcgag gcgttcgggc ccggcgcggt
     1741 gatggtgcgg gcgctgccgg cggcgctggg gtcgccggaa cctgggcctc tgctgcgcga
     1801 cctggctgat gagctcggcg agatggagga ggcgacgagc ctgcatgcgc ggctggatgc
     1861 ggtgatcgcg cggttggcgt gtcatgggtc ggtgcgggcg gggcggcggc tgaacgcggc
     1921 ggagatggac gcgctgctgc ggcagatgga ggcgacgccg cgggccgcca cgtgcagcca
     1981 tgggcggccg acgtttctca agctgaccaa ggcggaaata gagaagctgt ttgggcggcg
     2041 atagaaatta taacgcggag aaggccgagc atgcccccgg acttgatccg ggaggctcgg
     2101 ccttctccgc gttggatcct tctttctatc ccagcttgcc ggtgtatttt tccagcaagg
     2161 cccagggctc gtcgcccagc ttgtcgtgcc agagcttgta gaagccgcct tccttcaggc
     2221 cgtcgcggaa gctctgggtc tcggccgtgt tgaacaccat gccggacttc tcgagatcgc
     2281 cctgcaccga cttgtcgttg gtggcgatgt cggcgcgctc cagtagcgcc gcattgttca
     2341 ggttgcgggc gacgatggtc ttcagatcgt cgggcaggcc gttccaggcg gcggcgttgc
     2401 agcagagcca gtggccgtcc cagacgtggt tggtgagcga gcagtatttc tgcacctcgt
     2461 agaacttcgc caccttgatc agggtgagcg gattctcctg gccgtcggcg atgtgggtct
     2521 gcagcgcgga gtacagctcg gggaactgca aggcgagcgg ggccgccttc agggtctgga
     2581 acagcgtgac gaggctgggg gcgacagggg tacggatctt gaagcccacc agatcggccg
     2641 cgttgcgtat cggcttggtg ctgctggtga tctggcggaa gcccaggtcc cacatgcggt
     2701 ccatcgcaac gaggcccgat ttcgcggcga tctgcttacg tatatacgcg ccgagatcgc
     2761 cgtccatcgc cggccacacc ttggcatagt cggaccaggc gaagccgagc gcgctgatcg
     2821 cggtgaccgg caggatcgag gagaagatct ggccggtggg ctggcagaac tcgatagcgc
     2881 cctgacgggc ctgcgacagc aggtcgttgt cgccgcccaa ctggccgtcg gggaagacct
     2941 ggaagtccat gcggccgttg gattccttgt tgatctgcgc agcggcagcg gcgaggcgga
     3001 tgtggaaagg gaagctctgc ggcgcggtgt gcgcgaattt ccaggtgtat tcagcagccc
     3061 gcgcggtgcg cgccaggaag ggggaggcga gcacggcggc ggtgccgcca aggatgcggc
     3121 gtcgggtggg ctgcatgggc gtttccttcg ttgggcgtca gatggtgcgc ctttgtaggg
     3181 taggcgggcc ggcgtgcaag gggcggtaca cggcaaagtc ccggcggacg ccatccgcac
     3241 gcggctcggc cggctggtcc tgtgcccggt cagtgtcggc gatgattttc cgagccggtg
     3301 tgtccgtgtg acgtcctgcc gcggcgctgt ttttgccgct gcacgtgctg ttgccaggct
     3361 gcggatcggt cgcggatgaa gttgccgacg tccgcgcttg aagcggactg agcggacgag
     3421 gcgtcggctt cctcgtaatg accttgctcg ttgcggagcg gcaacgccgc gatgcgggct
     3481 gcatggagct tgtcgatctt cgacggctgt gacgaattgg gatggtgctc gcccgaaacg
     3541 ggtggcgcga cacctttgat aggatcatcg gtgcgtgcct gggccagggt gagagcatcc
     3601 gccagggagg ctgcagcctt gtcacgcgcg gtcagcgagg tggtgcccca tctgtcatgg
     3661 atcgtcctga ggacagaagt gtgatcgatg tggccgctgc cacgatagac agtgccagcc
     3721 ttgatgagag gtgagaccag gacagccggc gtgcggacac cgaaccgctt gaagtcgaag
     3781 ccgaactcgc cgacggtgcc atcgtctggc ggcagcgcat cggttggcgg cgcgacatga
     3841 tcgtagtttc cgccatgctc atcataggtg atgagcagaa gcgtcgacgc ccacgccctg
     3901 ccatttctca gcgcgtagta gacatcgtgg atgagctgtt cgccttttgc gacgtcgtag
     3961 ttcggatgct ggctgttgcc ggtggcatcc cagcttggct ccaggaatgt gtaggcaggc
     4021 agcgtcccgg ccgcagcgcg ttcctggaaa tcgcggaaat ggccgaaatg gttttcgtcg
     4081 gcattctgcg tgtcggggaa gtcgtggcgg gtcagcgggt cgcggttgta gccgtagatg
     4141 gcccagtcga tgttcttgtc ggagaggcgg ccaaagatgc tggggcaagt gaagatcttg
     4201 gtgtgatcat cgagatgtcc ctgcgaggta ccggccgcgg cgaaggcgcg gttgggcatt
     4261 gtcatggttg gtgctgacgc gaaccatctg tcgcagaccg cgtagccttt ggcgaggccg
     4321 gacatgatcg gcagcagctc cggagtatac atgcccatga tctggctggc ctgtgtgccg
     4381 ggcagcgtgt ccttgtagtg cttggccaga tcagaggcga tagcggcctt gaaattgatg
     4441 acgaaccctt tgttgtcggg aaccgcgcca ggatggggat catctgtcga gtagagctgg
     4501 tagttggtgt tgtagaagcc ctcgcccgga tcggcccctg gcatcaggta aggattggga
     4561 tcggttgcct tgatcttgta gaccttgatc tgccggccaa gatcgtcggg gttccattcg
     4621 tcaccggtga ggccatcgaa ggcatcgccc gttggcgatc gattgttgct gtcgctgtag
     4681 aggaagccca gcatctggtc gaaggaacgg ttctccagca tgagctggac aatgtgttcg
     4741 attttcccca gttggttcgc catgtcgcct tcctccgctt tgagtgtgag tgtccgggcg
     4801 ttgactgacg cgttatcgcg ttcagccgtc gtgtgtgtgt ggcgttacgg ttaaactgac
     4861 agttccgtcg gccgatgtga tcggccggaa ctgatcgaat gcctcgacct tgcagcagcg
     4921 gaaggattgc acatcagtca aacggcgggt gtgatctatg tcacatcggg acggggcgga
     4981 cggctgtcgc ggtggcaatt gcgacggagc tgtcgcggcg atcttccggt cgcaacaaga
     5041 ctgcacgctg gctgggacgc gaagactgat cagatggcga gtgcgctttg gtatgcgaaa
     5101 agagctggcg cgccaccggt gttgatgaag acgacatcgt cgtctttgtg ccagcgccct
     5161 tcgcggatga gagcgatcag ccccgcgaga ccttttcccg agtagacggg atcgaggggc
     5221 attgcttcca gccggccagc gagccgaatg gcctcgatgg tggcggcatg agggatgccg
     5281 taagcgggcc ccgcgtggcc ggccacgacc tcgacggcgg attcatcgaa cggcacgttg
     5341 aggagattgg cggcctcgcg cgcgaacctc accacgtcgg cgcgcacgcg atccggctcg
     5401 gcatcgatat caatgccgac aatgcgcgtg ttcggcatgg cgatggatgc gccgacgatc
     5461 aggccagcct gggttccggc gctccctgtg caatgcacga ttgcagtcgg atgaaagcct
     5521 tgcgcggagg cctggcttgc gatctcggcg attgtcgtcg cgtaggcgat cgcgccgagc
     5581 gcgttagaaa cgccatatgg cacgaaatac ggcctgtggc ctttcgcttc gaggttgttg
     5641 acgatcgcgc ggatggcttc gttgcggtcg ccggtccacg ggacgtcgtg caggtgcgcg
     5701 ccgaagagcc gattgaggaa ggcattgccc gaggtggtgt attctggtgt cggagccttg
     5761 agccggccgt gatagaccgc gaggtggcag gcgaggccaa gcttcgccgc ggcagccgcg
     5821 acctggcgtt ggctgttcga ctggacgacg ccgcccgaca cgatggtgtc cgcgccattc
     5881 gaaacggcat cgtgcaggac gaagtctagc ttgcgcagct tgttgccgcc gaaaccgatg
     5941 ccggtggcat cctcgcgctt gacccataac cgcgggccgc cgaggtgttc cgtcaggcgc
     6001 ttcatgggtt cgagcggggt cggcagatgc gccaacccca ggcgcgggaa agccgcgagc
     6061 cttgcggata aagtgccgtg aggggtgatc atgaatcact catggtcata gccgaccatc
     6121 gctcctgggc ttgcctgtat tctatgcagc ggcggtatgc taccgccgct ggcgccgtaa
     6181 aatgcaaagc cgacgcgcct gccgcacgat acgagcttgg acatatcccg gcgcggtgga
     6241 gaactgttgt gagagatcat gccaaggatc atgagacgct atcggacctc aaccggaact
     6301 atgtccggtc agtcgacgag gccgatacct cgtggttcga cgccaacctg gccaacgatt
     6361 tcctcaacac caatccagac ggcacattga tcgaccgcag cgcgttcatt gcccagatcg
     6421 gccgcggatc cttggtgaag gatattcgcg agcacgacgt cgtcatccgc attttcggtg
     6481 atttcgcgat catccatgcg cgcactgcgt acctcaagcc cgatggaaca caaggtggcg
     6541 gtcgctacac agacgattgg ttgttccgcg acggcagatg gcagtgcgtt tccgcccacg
     6601 ttgcacgcct ttgaggactt cgcaccatct tcagcgtccg tgtcctgcgg ttcacaggaa
     6661 cccgatcgag atccacggca ctgcggccac gatgatcagc cccacgatca gtgctgcgag
     6721 atagcgccag atgtggcgca tgccgtcatc ggggttgcag cggccgatag cgcaggcgac
     6781 gtagtagccg acgccgaagg gtggcgcgaa caggccgacg cccatcgcaa ggatcgcgac
     6841 catcgcgtag tgcacttcgt tgatgccgac ctgcttcgcc aatgggaaca gcagcgggcc
     6901 gaacagcacg atgacgggga tgccctccag cacgctgccc agcacgacga acagcaggat
     6961 ggagatcgcc atgaaggtga aggcgccgcc tggcatctcg gcgatccagc cggcgacgac
     7021 gcgcgagaag ccggagctgg tgattgccca ggccatcgag gtggcggcgc cgacaatcag
     7081 cagaatggcg ccggagaggg cggcggtctg caccagcatc ggcagcaggc gcgcgagatc
     7141 gaacctgcgg tagatcagca cgccggcgag cactgagtag acgatgccga tggtggagac
     7201 ctcggtcgcg gtggcgatgc cgccgacgac cgcgcctcgg atcacgaacg gcagcaccag
     7261 cgcgggaatg gcgatcagga agctgcggcc gatctcgcgg ctggaggcgc ggggcggcag
     7321 catgcggtcc tcgcggcggg cgcgccaacc caccatggcg cagaggatca ggccgacgac
     7381 gaggccaggc agcaggccgc cggtgaacag ggcggcgatc gagatcgagg tgaccgagcc
     7441 gatggtgatc aggatcaggc tgggcgggat cgtttcggtc tgcgcgccgg tggcggcgag
     7501 cagggcgacg aggtcgccgc gggtggcgcc gcgctgttcc atctcgggga acagcacggg
     7561 ggcgacggcg gccatgtcgg cggccttcga gccggagatg ccggagacga gatacatcgc
     7621 gccgatcagc acgaaggaga ggccgccgcg gacatggccg agcaggttgg cgagaaagcg
     7681 caccatgacc tgcgccatcc cggtcatgtc gatcagcagg ccgaggaaga cgaacagcgg
     7741 cacggagagc agcaccggat gcgacatgcc ggtgtccata cgcgcgacca gcaccgcgag
     7801 cggcacgtgg gtggcgaagg cgaggtagcc gagggtcgca agaccgaacg acacggcgat
     7861 cgggatgccg gcgaatacgc acgccgtgac gacgccgagg aagaagatca gcaggttgag
     7921 gcgacccagg gtgagcagcg tgggatggag gaacacgaag gctgcgatca gcacggcggc
     7981 agtgatgagc gccaggatcg tggcggggcg cagctgggtg agca
//
LOCUS       DATGEM010000168         7956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1684727, whole genome shotgun sequence.
ACCESSION   DATGEM010000168 DATGEM010000000
VERSION     DATGEM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684727"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VKQ27_05245"
     CDS             <1..853
                     /locus_tag="VKQ27_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HLY88370.1"
                     /translation="VATAVRSTVSEAAVRELTLKSETIAKSGGTPLAVCKDGRLLGVI
                     HLKDIIKGGIQERFSELRRMGIRTVMITGDNPLTAAAIAAESGVDDFLAQATPETKLK
                     LIREEQAQGKLVAMCGDGTNDAPALAQADVGVAMNTGTMAAREAGNMVDLDSDPTKLI
                     EIVEIGKQLLMTRGALTTFSIANDVAKYFAMIPAMFIAFYPQLQALNVMHLHNPQSAI
                     LSAIIFNALIIVALIPLALKGVTYKPIGAAAILRRNLLIYGVGGIIVPFVGIKLIDML
                     VTSIGLA"
     gene            867..1466
                     /locus_tag="VKQ27_05250"
     CDS             867..1466
                     /locus_tag="VKQ27_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HLY88371.1"
                     /translation="MKHIRPAIVMIVAMTIITGLIYPLAMTGIAQTVFPYQANGSLIT
                     KDGKVIGSALIGQNFTSEKYFHGRPSATTEPDPKDPTKTVPTPYAADNSAGSNLGPTS
                     KALVDRVKADAATLAKENPGTPIPSDLVTTSASGLDPDITPAGALFQVPRVAKARGLP
                     EDQVRQLVESHITGRLLGIIGEPHVNVLKLNLALDAMKS"
     gene            1576..4284
                     /locus_tag="VKQ27_05255"
     CDS             1576..4284
                     /locus_tag="VKQ27_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HLY88372.1"
                     /translation="MDDRSSREGRPSPDALLQDADQGSRGRLKIFMGAAPGVGKTFEM
                     LSAAQARRHEGADVVVGVVETHGRKETVALLDGLEAIPRRPVEYRGHTLYEMDLDALL
                     ARRPQLALVDELAHTNAAGSRHPKRYMDVEELIAAGIDVYTTLNVQHVESLNDVVARI
                     TRIRVRETVPDSIIDKADDIEVIDLTPDDLIQRLKDGKVYVPHQAERAVRHYFSQGNL
                     TALRELALRRTAQRVDDEMVRYMQAHAIPGPWAAGERVLACIGSDPSSTDVVRLAKRM
                     ADSLRAPWTAIHIETGRNLNLSDAQRDRIAEAMRLAERLGAETVTLPGQDVAGTIADY
                     ARANNFTHIIIVQPPRSIWRELLQGSLAQQLIRQAGGASVHVVGRTREASELSDQPIL
                     REAARHAGDARAYLGSLAFVAVSLGAALLLHQMLSLASVAMAFITAVLVAAVVYGLWP
                     ALFASFLSVLAYNFFFLPPLYTFTIEDPENVAALFFFAVTAVIASNLTARVRAQAIVA
                     RQRARTTEELYQFSRKLAGAANLDDLLWATVHQIALMLKVRVVILLPEEGTVTVRAGF
                     PPEDTLDAADVAAARWSWEKNHVAGRGSDTLPGAKRLFKPMNTGRGAVGVIGIDSDEP
                     GPILTPDQHRLLDALSDQAALAIERVNLVEDVDRARLAVETDRLRAALLTSISHDLRT
                     PLASILGSATSLSSQGDTLSASTHSDLIRNIQDEAERLNRFIGNLLDMTRLESGPLRL
                     NTGPVELSDVIGSALHRARKILANHRTQVTLQPDLPMLDVDEVLFEQVLFNLLDNAGK
                     YAPAGSLVTVQAWRDDGRIGVQVLDEGSGIPDVDLEHVFDKFYRTGPADRRRAGTGLG
                     LAICRGFVEAMNGTIVAGNRTDRSGAVFTIKLPAFVNAERPAETIE"
     gene            4281..4979
                     /locus_tag="VKQ27_05260"
     CDS             4281..4979
                     /locus_tag="VKQ27_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY88373.1"
                     /translation="MSAGSAPTRILVVDDEPAILRFLRAGLGSQNYLVIEAQTGQAAI
                     DAARQHRADLIVLDLGLPDIDGLEVIRHLREAGVALPIIVLSSRENEAAKVTALDLGA
                     DDYITKPFGIDELLARIRAAQRHRLQQQGERPIFRTGDLTVDLVRRIVTVRGQEVKFS
                     PREYDLLRLLVAHAGKVLTHNMILREVWGPNTDVQYLRIYIRALRQKIERDTEKPEYI
                     QTETGVGYRLRAPD"
     gene            4979..5425
                     /locus_tag="VKQ27_05265"
     CDS             4979..5425
                     /locus_tag="VKQ27_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLY88374.1"
                     /translation="MKIGDIIAPDRVIVGLRAADKTQLLQDLAGRAATVLSMERRKIF
                     DALLARENLGSTGLGKGFALPHARLEGLGGSFALFVRLARPIDFASIDERPVDLAILL
                     LTPANGANHHLATLAALSRPLRDDLFVQKLRAAPDATALHRLLASA"
     gene            5552..7297
                     /locus_tag="VKQ27_05270"
     CDS             5552..7297
                     /locus_tag="VKQ27_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HLY88375.1"
                     /translation="MHSLPPPEAVRHLVPLVERAIQAHRQGANETAERLCLDVLELAP
                     DRPGALSVLYDIRKAQGNARAAEMLIRRIVALDPNNLVATNEIALILLGKGSLAEAEH
                     HARNAVRIAPENPQAHNLMGMIMTEANRPQIGEYHYRRVMELTGQRDPILLANLAWNL
                     KNQGRMKEARSLYEESVTAAPQVRQTLLGFARLEEADRDFDAAAELLDKADAQFPNDQ
                     GVRLTRAVLLGRMRRYDEALAIIDATADDEGRLGPNELLEKGLLLDQLGRYDDAWTAF
                     AEGKRLARELSGQAYLENDANAQIDRLRNFFTSGRLRLMPRANVRSDVPQPIFILGFP
                     RSGTTLIEQTLSAHPKIAAGDELPLIHEITAIMPRMLASPLGYPEALAELWMGDQREG
                     LDNLRDYYLQKVRQMGVLRSGATRFTDKMPLNETHLGLIALLFPEAPLIHVLRHPLDI
                     MISAFSNQFTHGFFCAYDLETAARHYVRVMDLVQHYRAEMSLRYLPIRYEDIVLDQEA
                     SVRAVLAFVGEPFNKACLTFHKNRRYARTASYAQVAEPLYQRSVHRYRHYMKHLEPVI
                     PILQPVIERLGYTVD"
     gene            7342..>7956
                     /locus_tag="VKQ27_05275"
     CDS             7342..>7956
                     /locus_tag="VKQ27_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88376.1"
                     /translation="MPLPVGDVLRIANEYERGGRLDDAKRLLDHVLAASPNQGDALHL
                     SGIVAFRQGDIHKSLELMERSLTFGIDTPLYLRNICEVYRSLGRLDEALATARRATML
                     APADPLCLHNQAIIHYHRLELDEALDCAGQALRIDPSLPGAHFVRAEALLLRGDWAEG
                     WEEYEWRFRIAGAAPLMPPTTRQQWDGSAFSDQPLLLIADQGFAD"
BASE COUNT         1465 a         2608 c         2487 g         1396 t
ORIGIN      
        1 cgttgccacg gcggtgcgtt ccaccgtttc ggaagccgcg gtgcgcgagc tgaccctgaa
       61 gtccgagacg atcgcgaagt cgggcggcac accgcttgcc gtctgcaagg atgggcgtct
      121 gctcggcgtg atccatctca aggacatcat caagggcggc atccaggagc gcttttccga
      181 gctgcggcgc atgggcatcc gtaccgtgat gatcaccggc gacaacccgc ttaccgccgc
      241 agcgatcgcc gctgaatctg gcgtcgatga cttcctggcc caggcaacac ccgagaccaa
      301 gctcaagctg atccgcgaag agcaggcgca gggcaagctt gtcgcgatgt gtggtgacgg
      361 caccaacgat gcaccggcac tggctcaggc cgatgtgggc gtggcgatga acaccggcac
      421 gatggcggca cgcgaagccg gcaacatggt cgatctcgac agcgatccca ccaagctcat
      481 cgagatcgtc gagatcggca aacagctcct gatgacgcgc ggcgcgctga ccacgttcag
      541 tatcgcaaac gatgtcgcga agtatttcgc catgatcccg gcgatgttca tcgcattcta
      601 cccgcagctg caggcgctga acgtgatgca cctgcacaac ccgcaaagcg ccatcctgtc
      661 agcgatcatc ttcaacgcgc tgatcatcgt ggcgctgatc ccgctggcgc tgaagggcgt
      721 cacctacaag ccgatcggcg cggcggcaat cctgcggcgc aacctgctca tctatggcgt
      781 tggcggcatc atcgtgccgt tcgtcgggat caagctgatc gacatgctcg taacctccat
      841 tggtctggcc tgaggggcca cgaaacatga aacacattcg acccgcgatc gtcatgatcg
      901 tcgcaatgac catcatcacc ggtctgatct acccgcttgc catgaccggc atagcccaga
      961 cggtctttcc ctatcaggcc aatggcagcc tgataacgaa ggacggcaag gtcatcggtt
     1021 cggctctgat cgggcagaac ttcacatcgg aaaaatactt ccatggccgc ccgtcggcga
     1081 ccacggagcc tgatccgaag gatcctacca agaccgtgcc gacgccctac gccgccgaca
     1141 actcggctgg ttccaacctt ggtccgacgt cgaaggctct ggtggatcgg gtgaaggctg
     1201 acgcagccac gctggcgaag gaaaaccccg gcacgccgat cccgtccgat ctggtgacaa
     1261 cgtcggcaag cggccttgat ccggacatca caccggccgg cgcgctgttc caggtgcctc
     1321 gcgtcgcaaa ggcgcgcggc ttgcccgagg atcaggtgcg gcagttggtg gaaagccaca
     1381 tcaccggccg gcttctcggg atcatcggcg agccgcatgt gaatgtcctg aagctgaacc
     1441 tcgcgctgga cgcgatgaag agctaacttc gtcctgtcat cgcgaggaag cgaagctgac
     1501 gaagcaatct cgtccgggat tgcttcgtca gctttctcgc ctcgcgatga caagagcggc
     1561 agatggattg ttgcgttgga cgatcgaagt tcccgcgaag gccgaccatc tcccgacgcc
     1621 ctcctgcagg acgcggacca aggttcacgt ggccgcctga agatcttcat gggcgccgct
     1681 ccaggcgtcg gcaagacatt cgaaatgctg tccgcagccc aggcccgtcg gcatgaaggc
     1741 gccgacgtgg ttgtgggagt cgtagaaacg cacggccgca aggaaaccgt ggcgctgctg
     1801 gacggattgg aagccatccc gcgtcgcccc gtcgaatacc gcggtcacac gctctatgag
     1861 atggacctcg atgcgctgct ggcgcgacgc ccacagcttg cgctggtcga tgagcttgcc
     1921 cacaccaacg ccgccggcag ccgccatccg aagcgctaca tggatgtcga ggaactgatc
     1981 gccgccggca tcgatgtata cacaaccctg aacgtccagc acgtcgaaag cctgaacgat
     2041 gtggtggcac gcatcacccg catccgcgtg cgcgagacgg tgcccgactc aatcatcgac
     2101 aaggcggacg acatcgaggt gatcgacctc acgccggacg atctgatcca gcgcctgaag
     2161 gacggcaagg tctatgtgcc gcatcaggcg gagcgcgcgg tccgccacta cttctcgcaa
     2221 ggcaacctga cggcattgcg cgaactggct ctccgacgca ccgcgcagcg ggtagacgat
     2281 gagatggtgc gctatatgca ggcgcacgcc atcccagggc catgggcggc gggcgagcgc
     2341 gtgctcgcct gtattggctc ggatcccagc agcaccgacg tggtgcggct tgccaaacgc
     2401 atggctgaca gcttgcgagc cccctggacc gccatccaca tcgagaccgg ccgcaatctc
     2461 aacctgagcg atgcccagcg cgaccgtatc gccgaggcga tgcgtctcgc cgaacgtctc
     2521 ggcgcggaga ccgtgactct gcctggtcag gacgtcgccg gcaccatcgc ggactatgca
     2581 cgcgccaaca actttaccca catcatcatc gtgcagccgc cgcgctcgat ctggcgcgaa
     2641 ctgctccaag gatcgctggc acagcagctc attcggcagg ccggcggggc cagcgtgcac
     2701 gtcgtcgggc gtacacgcga agcctccgag ctgtccgatc agccaatcct gcgcgaagca
     2761 gcgcggcatg cgggcgatgc gcgcgcttat ctcggcagcc tcgcatttgt cgctgtgtcg
     2821 ctgggcgccg cgctgctgct gcatcagatg ctcagcctcg ccagcgtggc gatggccttc
     2881 atcacggccg tcctggtggc cgctgtcgtc tacggcctgt ggccggcgtt gttcgccagc
     2941 ttcctcagcg tattggccta caacttcttc ttcctgccgc cactctacac cttcaccatc
     3001 gaggaccccg agaacgtcgc ggcactgttc ttcttcgccg taaccgcggt aatcgccagc
     3061 aacctgaccg cacgtgttcg cgcccaggcg atcgtggcgc ggcagcgtgc ccgcaccacc
     3121 gaggaactct accagttcag tcgcaagctt gccggcgcag cgaacctgga cgacctgctg
     3181 tgggcgacag tgcatcagat cgcgctgatg ctgaaagtgc gcgtggtgat cttgctgccc
     3241 gaggaaggca ctgtcacggt ccgcgccggc ttccccccgg aggataccct ggatgccgcg
     3301 gatgttgcgg cagcgcgctg gtcctgggag aagaaccacg tcgccggacg cggttcggac
     3361 acgctgcccg gtgccaagcg cctgttcaag ccgatgaaca cgggacgcgg cgccgtcggt
     3421 gtcatcggca tcgacagcga tgaaccgggt cccatcctca cgcccgacca gcatcgcctg
     3481 ctcgatgcac tgtccgacca ggcggcactg gcgatcgagc gcgtcaacct cgtggaagac
     3541 gtcgaccgcg cccgccttgc cgtggagacc gacaggttgc gcgcggcgtt gctgacgtcg
     3601 atctcgcatg atctccgcac gccgctggcg tcgatcctcg gttcggcgac cagcctgtcg
     3661 tcgcagggcg atacgttgag cgcatcaact cactctgatc tcatccgcaa catccaggac
     3721 gaggcggaac ggctgaatcg attcatcggc aatctgctcg acatgacgcg gctcgaaagc
     3781 ggtccgctac gcctgaacac cggcccggtc gagctctccg acgttatcgg cagcgcgctg
     3841 catcgtgccc gcaagatcct cgcgaaccat cgcacgcagg tcacattgca gcccgatctg
     3901 cccatgttgg acgttgatga agtgctgttc gaacaggtgc tgttcaatct gctcgataat
     3961 gccgggaaat atgcgccagc cggatcgctg gtgacggtgc aggcgtggcg cgacgatggc
     4021 cgtatcggcg tacaggtgct ggatgaaggt tccggcattc ccgacgtgga tctggagcac
     4081 gtattcgaca aattctatcg caccggtccc gccgatcgcc ggcgcgcggg tacgggcctt
     4141 ggtctcgcca tctgccgcgg cttcgtcgag gcgatgaacg ggacgatcgt cgccggcaac
     4201 cgcaccgacc ggtccggcgc ggtcttcaca attaaactgc cggcgttcgt caacgcggaa
     4261 cggccggcgg agacgatcga atgagcgccg gatccgcacc gacgcgcatc cttgtggttg
     4321 acgacgaacc ggcgatcctt cgcttcctgc gtgccggtct cggcagccag aactaccttg
     4381 tgatcgaggc gcagaccggc caggccgcca tagatgcggc gcgccagcat cgcgccgacc
     4441 tgatcgtgtt ggacctaggt ctgcctgaca tcgacggcct tgaagtcatc cgccacctcc
     4501 gcgaggctgg cgttgccttg ccgatcatcg tgctgtccag ccgcgagaac gaagcggcga
     4561 aggtgaccgc gctagatctt ggcgccgacg attacatcac caagccattt ggcatcgacg
     4621 agttgctcgc gcgcatccgt gccgcacagc ggcatcgcct gcagcagcaa ggagagcggc
     4681 cgatcttccg caccggcgat ctgacggtgg acctggtccg ccgtatcgtc acggtccgcg
     4741 gtcaggaggt gaagttctcg ccacgcgaat acgacctgct gcggctgctg gtggcccacg
     4801 ccgggaaagt gctcacccac aacatgatcc tgcgcgaggt ttgggggccc aacacagatg
     4861 tgcaatatct gcggatctat atccgggcat tgcgccagaa gatcgagcgc gataccgaga
     4921 agcccgagta catccaaacg gagaccggcg tgggttaccg cctgcgagcc cctgactgat
     4981 gaagattggc gacatcatag cacctgatcg ggtcatcgtc gggctgcgcg ccgccgataa
     5041 gacgcaattg ctgcaggatc tggccggccg tgcggcgacc gttctgtcaa tggagcgccg
     5101 caagatcttc gatgccttgc tggcacgcga aaacctcggt tcgacaggtc ttggcaaagg
     5161 atttgccctg ccgcacgcgc ggttggaagg cctgggcggc tcgttcgcat tgttcgtacg
     5221 tctcgcccgg ccgatcgact tcgcatcgat cgacgagcgt ccggtggacc tggccattct
     5281 gctgctgaca ccggccaatg gcgccaatca ccacctggcg acattggccg cgctttcccg
     5341 gccgctgcgc gacgatttgt tcgtgcagaa gctgcgtgcc gcgccggatg ccaccgccct
     5401 gcaccggtta ctcgcgagcg cgtgaccgtg cttgccccgg cggggcgccg cgtgcgagaa
     5461 ccatcgtgga attatgaatg cgccagcagc agtttcgcac tcgtgcccgt gcggcagtgg
     5521 cctgcgctca gtgcgctgtt gcagtctgca gatgcactcg ctgccaccac ccgaagccgt
     5581 ccgccacctg gtccctctgg tagagcgcgc gatccaggcg catcgccagg gcgccaacga
     5641 aacagcggag cgcctctgcc tggatgtgct ggaactggcg cccgaccggc cgggtgcact
     5701 gtctgtcctg tacgatattc gcaaggcaca ggggaacgcg cgcgccgcgg agatgctgat
     5761 ccgccggatc gttgcgctgg atcccaacaa tctcgttgcc accaacgaaa tcgcactgat
     5821 cctgttgggc aaaggcagtc tggccgaagc cgagcatcat gcccgcaacg ccgtgcgcat
     5881 cgcgccggag aacccgcagg cacacaacct gatgggcatg atcatgacgg aggcgaaccg
     5941 gccgcagatc ggcgaatatc attaccgccg cgtgatggaa ttgaccgggc agcgagatcc
     6001 gatcctgctg gcaaatctgg cgtggaatct gaagaatcag ggccgcatga aagaggcgcg
     6061 gtcgctgtac gaggagtcgg tgacggccgc gccacaggtg cgtcagacat tgcttggttt
     6121 tgctcggctg gaggaagcgg accgcgactt cgatgcagcc gctgaactgc tcgacaaggc
     6181 ggacgcgcag tttcccaacg atcagggcgt ccggctgacg cgcgccgtgt tgctgggccg
     6241 catgcggcgc tacgacgaag cgctggcaat catcgatgca accgccgacg acgaaggacg
     6301 gcttggtccg aacgaactgc tggagaaggg gctcttgctc gaccagcttg gacgctacga
     6361 cgatgcctgg accgccttcg cggaaggcaa gcgattggcg cgcgaactgt ccggccaggc
     6421 gtatctggaa aacgacgcga acgcgcagat cgaccggctg cggaacttct tcacctcagg
     6481 ccgcctgcgg ctgatgccgc gtgccaatgt gcgttccgat gtgccgcagc cgatcttcat
     6541 cctgggattt ccgcgctctg gcacaacgtt gatcgaacag acgctctcgg cgcatccaaa
     6601 gattgccgcg ggtgatgagc tgccgctgat ccacgagatc actgcgatca tgccgcgcat
     6661 gctggccagc ccgctcggct atcccgaggc gttggccgaa ctctggatgg gcgaccagcg
     6721 cgaagggctg gataacctgc gggactacta cctgcagaag gtgcggcaga tgggcgtgct
     6781 gcgcagcggc gccacgcgct tcaccgacaa gatgccgctg aatgagacgc atctcgggct
     6841 gatcgcgctg ctgttccccg aggcaccgct gatccatgtg ctgcgccatc cgctcgatat
     6901 catgatatcg gcattctcca accagttcac gcacggcttc ttctgtgcct atgacttgga
     6961 gaccgcggcg cgtcactacg tgcgcgtgat ggacctggta cagcattacc gggccgaaat
     7021 gtcgctgcgc tacctgccga ttcggtatga ggacatcgta cttgaccagg aggcaagcgt
     7081 gcgggccgtg ctggccttcg ttggcgagcc tttcaacaag gcctgtctga ccttccacaa
     7141 gaaccggcgt tacgcccgca ccgcaagcta cgcgcaggtt gccgagccac tgtatcagcg
     7201 ctcggtacat cgctaccgcc attacatgaa gcacctggag ccggtgatcc cgatcctgca
     7261 gccggtgatc gaacggctcg gctacacggt cgactgatgg agacgcccgc gcccgccccc
     7321 cgggcgtccg cgcagggcac cgtgcctttg ccggtcggcg acgttctccg catcgccaac
     7381 gagtatgagc gtggcggccg gctcgatgat gcgaaacggt tgctcgatca tgtcctggct
     7441 gcatcgccga atcaaggcga tgcgctgcac ctgtcaggca tcgtcgcgtt ccgccagggc
     7501 gacatccaca agtccctgga gctgatggaa cgctcgctca cgttcggcat cgatacaccg
     7561 ctctacctgc gcaatatctg cgaggtctat cgctcgctgg gacgcctgga tgaagcgctg
     7621 gccaccgcgc gcagggccac gatgctggcc ccggccgatc cgctctgcct gcacaatcag
     7681 gccatcatcc actaccaccg gctggagctg gacgaggcgc tcgattgcgc cggccaggcg
     7741 ctgcgcatcg accccagcct gcccggcgcg catttcgtgc gggccgaggc gctgctgttg
     7801 cgcggtgatt gggccgaggg ttgggaggaa tacgagtggc gcttccgcat cgccggcgcc
     7861 gcgccgctga tgccgcccac gaccaggcag caatgggacg gcagcgcgtt ctccgaccag
     7921 ccgctgctgc tgatcgcgga tcaggggttt gccgat
//
LOCUS       DATGEM010000169        11386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1435595, whole genome shotgun sequence.
ACCESSION   DATGEM010000169 DATGEM010000000
VERSION     DATGEM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435595"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VKQ27_05280"
     CDS             <1..580
                     /locus_tag="VKQ27_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HLY88377.1"
                     /translation="NVMRTVQELETAGVAAMCIEDTLLPRAYGEKQPSLISLEEGVGK
                     MRAALAAREDPSFVIVGRTSAAAIAGTEDVVRRAKAYAEVGVDALFFAGGMTREQLDA
                     IASAVSIPLMLGGSGRELQDRDYLASRRVRIALQGHQPFSAAVLAVYNTLKALREGTK
                     PADLPNIASADMMKRVSREGDYKSWTEKFLGG"
     gene            881..2218
                     /locus_tag="VKQ27_05285"
     CDS             881..2218
                     /locus_tag="VKQ27_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY88378.1"
                     /translation="MVSLRRPAYCPPHSMSPPLELVQSDPEVPARAGVVIIGGGIIGA
                     STALFLAEKGHSVVLCEKGRIGGEQSSRNWGWCRTMGRDADEIPLAMESLRLWRGMNE
                     RTGRETGYRQAGIMYLCETEQEMADREAWLVEAEQYQVDARVVRGAAMDDVIPGASRL
                     FIGALHTPTDGRAEPSMAAPAIAEAARAAGAAVLTDCAVRGVETKGGRVSGVVTEKGR
                     IACDVAVLAGGAWSRLFCGNAGIELPQLKVLASVFRTQPLDGGPETAASGSVFAFRKR
                     LDGGYTIARRDANIADITPDSFRLLRQYLPTLRENYGEIRMRIGRRFLEEWRTRRRWS
                     LDEPTPFEAVRVWDPEPRHAVLEEARKVLSQKFPAFAGMQIAQSWGGMIDVTPDAVPV
                     IGEVPSLPGFFLSTGFSGHGFGIGPGAGRLTADLVSGDTPIVDPHPFRFGRFS"
     gene            2407..2601
                     /locus_tag="VKQ27_05290"
                     /pseudo
     CDS             2407..2601
                     /locus_tag="VKQ27_05290"
                     /inference="COORDINATES: protein motif:HMM:NF039792.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6471 domain-containing protein"
     gene            2694..3224
                     /locus_tag="VKQ27_05295"
     CDS             2694..3224
                     /locus_tag="VKQ27_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY88379.1"
                     /translation="MPDWLASVPELTNDKTIQPGEGMMPGYVLTENDGEHVFQRSGEI
                     VIKIDPTRGSHELCLGTQRMPVGIGVPRHVHAYWDEHIYVLDGSGYITLDDERVPIQK
                     GATVFIPKGCWHGFENPDSELFLIWAAAPTGQEEFFRAISSKPGEPPKNLSREEVLAI
                     RHQVEADYLKRVQAQA"
     gene            complement(3246..3524)
                     /locus_tag="VKQ27_05300"
     CDS             complement(3246..3524)
                     /locus_tag="VKQ27_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLY88380.1"
                     /translation="MTRPNNLVGMQHRYFVYILTNSRRTVLYTGVTNDPLRRVSEHRD
                     PGFKGFTHRYDVNQLVYYEEFGDSLAAIAREKLGLMAQTPKSRLPRRC"
     gene            3551..4489
                     /locus_tag="VKQ27_05305"
     CDS             3551..4489
                     /locus_tag="VKQ27_05305"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLY88381.1"
                     /translation="MTQTAILTKPATIPLAVRQRVLFGILCFVWGTTWLGMKVGIAVV
                     PPGVFSGTRWTMAGLTLLLWRWARGQRLLIPMRLTGRLLIVAVLMVSLNQVIQLYGLR
                     HITAGLACVLSSALTPIALLGFAVAWGQERFAWRQLVALGIGIAGVLVLFGPAAVAGK
                     LDAWEVLGAAGVIIGTMCYSAGSVMARPLMNSLAPAQMAAMTNLIGGVLLLISSIVFE
                     PGAASALIHPWGWAASIAWLYLLIPGSLLSTVIYFLLVRDWGASRTGTYAFVSPVVAV
                     VIGTSLFGEKLDLGDVVGMVLMLVAAAIVLRRPIVP"
     gene            complement(4476..5264)
                     /locus_tag="VKQ27_05310"
     CDS             complement(4476..5264)
                     /locus_tag="VKQ27_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY88382.1"
                     /translation="MPLLCDRHGTIAVLTLSRPEARNAWCEEFQTGLVQLLPELEADP
                     DVRCVILTGDDKGGAFSAGADLKNPRTHTMDSVAAFIQDLPNRRRAHPIPALTDFAKP
                     IIAAVNGYAVGIGCIVTYCCDMIVASERAEWRLPQSGLGIIPAQGGATRLARWVGKGL
                     AMRLALGFPLKADEAYRIGLAQWLVPHDALMAQAMEVGEHIASLPPLATRLTKESLNR
                     GADVPLADAVLGDLYRFAMLELTEDKHEAHQAWRERRKPRFKGR"
     gene            complement(5335..6048)
                     /locus_tag="VKQ27_05315"
     CDS             complement(5335..6048)
                     /locus_tag="VKQ27_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY88383.1"
                     /translation="MDIVLVHSSDVHVDDETSATKHHDGTAGLAAVLAAARAHNADIV
                     LLAGDTFDNNRVPVPVLDRVGRLLSDAGMPVVILPGNHDPAMPNSVFQRGGLGSIQGV
                     SIFGVTHEEATAFQAHDLEIWGHAHRDYENMAPLRGPRARTTRWQIAMAHGHYEPPHN
                     LATPLRPSWLFTDTHIAETQADYLALGHWDRPAQVGDGSVPAYYSGSPDLAQTVNVIR
                     LTSGGEVLVSREPLLLDTP"
     gene            complement(6126..7616)
                     /locus_tag="VKQ27_05320"
     CDS             complement(6126..7616)
                     /locus_tag="VKQ27_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88384.1"
                     /translation="MPSEAIEPLPEPDLLPANDEDAPSPLLESGIFAPATPNDVQALV
                     DTPCRALEVEYKGWRNLSNGEDRAELARDIAALANHGGGHIIFGFDQDTLTPEDTDPF
                     VTRCSPEQVSAIVRTYLDPPVACEVATLRSATGSAHPAIRVAGHGPVPICVRQDGPLV
                     GPSRLLERGSFYIRKHGAVAGAFIGIPRPRTERIETPQDWAPLIRRCVRQDREALLAM
                     IGAAVEGRANVPDVVERLAIWHAAAHAAFLRLVPLSPVAEQLGRAHYMLSYAFELAGP
                     AVPDQAQLPDILRRVVFELRPLFRDSMMLFDPPYRRSVQPRFVVDDATGDNETDFLEV
                     AWLRERPPGETADFWRLSPRGLASIVRDYPEDKTDANEALGMQPGTWFSPNVLAQDVA
                     ALVCHARSLARFFASVRRVHFRCEWWGLAGREVFDPHASWVQRGAVGDDHRVVTTQMP
                     AASLGQAWPEMVAQIMAPVLRAFEPDLALGGDWVRGQMARWAQAGG"
     gene            complement(7741..8115)
                     /locus_tag="VKQ27_05325"
     CDS             complement(7741..8115)
                     /locus_tag="VKQ27_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HLY88385.1"
                     /translation="MQRLWMGIGALAGLSAVAMAALAAHGLDRLDPPSLQMLRNALQM
                     HGWHALALLACGLWAPRGGRLADAAGGVFTVGLVLFCGAVYALALDGIRLPLFAPIGG
                     TLLMTGWILLFVSILLTSPLRR"
     gene            8163..9113
                     /gene="pip"
                     /locus_tag="VKQ27_05330"
     CDS             8163..9113
                     /gene="pip"
                     /locus_tag="VKQ27_05330"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HLY88386.1"
                     /translation="MPRGDLFPEIGPFETGYLPLSSNHVMYWEQVGNPRGRPVLFLHG
                     GPGAGAGAVHRRFFDPEFWRVIIFDQRGAGRSRPLGSLDDNTTQDLVSDIETLRRHLG
                     LERWLLFGGSWGSTLALAYAQACPERVIGCVLRGVFLGRPVEVDWFLDGLAAIFPDAH
                     AAFVGFLPEEERAHVLGGYLSRLTDPDPAVHLPAARAWSIYEGSCSTLLPSPETVTSF
                     AQDRTALGLARIEAYYFAHGLFLPPEGLLGNMHRIGRIPAEIVQGRYDMICPARTAFE
                     LAAAWPAARLSIVPDAGHSALEPGIRRALVAAVERFRQGR"
     gene            9119..10000
                     /gene="ltnD"
                     /locus_tag="VKQ27_05335"
     CDS             9119..10000
                     /gene="ltnD"
                     /locus_tag="VKQ27_05335"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HLY88387.1"
                     /translation="MKVGVIGLGTIGLGAALNLARKGHQVVGCDVREAARAEIPTAVA
                     HPDELPADTEAVAVFVVNAAQTEDVLFGARGCLAKLAKGVVVLSCATIAPEAARALDA
                     KISAAGCLMLDAPTSGGKVGAHGGTMTVMASGAEAAFAKAQPVLDAISTKVWRLGDAV
                     GVGSTVKMVNQLLAGVHIATAAEAMALGIRAGADPRTLFEVISSSAGSSWMWQNRMPH
                     VLEGDDTPLSAVNIFVKDLGIVLDQARALTFPLPMASAAHQLFLAAAAHGEGAKDDAF
                     VIRVWQALAGIDLPKGG"
     gene            10051..10935
                     /locus_tag="VKQ27_05340"
     CDS             10051..10935
                     /locus_tag="VKQ27_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLY88388.1"
                     /translation="MADPQKPGQRLDYQASIDRPRLALPDGKRVAVFIVVNVENWDID
                     RAMPRQVLTAPQGASVVPDLPNWAWHEYGMRVGIWRIKAALDEHGIAPSMTINGTVPA
                     AYPRVARAARDAGWEFMGHGFRQMATHLVDDQRAMIRDAVSALREAAGHDIVGWLAPG
                     LTETLDTPDLLAEAGIRYCADWVIDDLPCTLRTAHGLILTMPYSVELNDIPMMMIQHH
                     RAEELFARTVAQFNRLYAEAETQGAKVMGLAVHPYISGVPHRIGWFEQALSYMASKPG
                     TLFWQGRQIMDWYLKAAA"
     gene            10932..>11386
                     /locus_tag="VKQ27_05345"
     CDS             10932..>11386
                     /locus_tag="VKQ27_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY88389.1"
                     /translation="MNWGAMSRAERDAAYNNSEAVKNSPQLNAARAAASAEFRAAHPG
                     HLDLPYGPRERNTWDLFPAKDPNAPCIVFIHGGYWQRNSKDQFANLIAGPYAHGWAGA
                     LPGYTLGPDATLTEITAEINAALDWLVANGPSHGISGPIVLSGWSAGGHL"
BASE COUNT         1939 a         3600 c         3771 g         2076 t
ORIGIN      
        1 caacgtgatg cgcacggtgc aggagctgga aaccgccggt gttgctgcga tgtgcatcga
       61 ggacacgctg ctgccgcgcg cctatggcga gaagcagccg agcctgatct cgctggagga
      121 aggcgtcggc aagatgcgcg cggcgttggc ggcgcgcgag gatccgtcgt tcgtcatcgt
      181 cggccgcacc agcgcggccg cgatcgccgg taccgaagac gtggtgcgcc gcgcgaaggc
      241 ctatgccgag gttggtgtcg atgccctgtt cttcgctggc ggcatgacgc gcgagcagct
      301 tgatgcgatc gcgtcagccg tgagcatccc gctgatgctt ggcggttccg gccgcgagct
      361 gcaggacagg gactacctcg ccagccgccg tgtgcgcatc gcgctgcaag gccaccaacc
      421 tttctcggct gcggtgctcg ccgtctacaa tacgctcaag gcgctgagag aaggcaccaa
      481 gcctgccgac ctgcccaaca tcgcctctgc cgacatgatg aagcgggtct cgcgtgaggg
      541 cgactacaag agctggacgg agaaattcct cggaggctga ggatcccagc gccagcggcg
      601 cgatgcctgc cacaggcatc gcgccgtcgc ggacgctcgg ggtccggcta ctcgcagtcg
      661 cagccaaatc tgccgacacc agaccctgac gacgaggacg catctgcgac catgacggta
      721 agcggcagca gtgccattgc caggaaagcc aacagagcca gaacacgaat acgcattgga
      781 taacccccca tcagcggttg cgcgaccgtt gcggtcgctc agacgcttac cccgcccgca
      841 ggcgttttat tccccgcaat gctccagtga gcgtacgtca atggtcagcc tgcgccggcc
      901 ggcctattgt ccgccccatt ccatgtcgcc gcccctcgaa ctcgtccagt ccgaccccga
      961 agtcccggcc cgcgctgggg tggtcatcat tggcggcggc atcatcggtg ccagcaccgc
     1021 gctgttcctg gccgagaaag gccactctgt cgtgttgtgc gaaaagggcc gcatcggcgg
     1081 agaacagtcc agccgcaact ggggctggtg ccgcactatg ggccgcgatg ccgacgaaat
     1141 tccgctggca atggaaagcc tgcgcctctg gagagggatg aacgagcgca caggccgtga
     1201 gaccggctac cgccaggccg gcatcatgta tctgtgcgaa accgagcagg agatggcgga
     1261 ccgcgaggct tggctcgtgg aggcggagca gtatcaggta gatgcgcggg tggtacgcgg
     1321 cgccgcgatg gatgacgtga ttcccggtgc atcgcgtctg ttcatcggcg cgctgcacac
     1381 accgaccgat gggcgcgcgg aaccgtcgat ggcggcaccg gctatcgccg aggcggcacg
     1441 tgccgccggc gccgccgtgc tgacggactg tgctgtgcgc ggcgtggaga ccaagggcgg
     1501 gcgggtatcc ggcgtggtga ccgagaaggg gcgtatcgcc tgcgatgtgg ctgtgctggc
     1561 cggcggcgca tggtcgcgcc tgttctgcgg caacgcgggc atcgagttgc cgcagctgaa
     1621 ggtgctggca tccgtgttcc gcacccagcc gctcgacggt ggtccggaga cggcagccag
     1681 tgggtcggtg tttgcatttc gtaagcggct cgatggcggc tacacgatcg cccggcgcga
     1741 cgcgaacatc gcggacatca cgcctgattc gttccggttg ctgcggcaat accttccgac
     1801 cttgcgcgag aactatggcg agatccgcat gcggattggc cggcgcttcc tcgaggagtg
     1861 gcgaaccagg cgccgctggt cactggatga accgacgccg ttcgaggcgg tgcgcgtgtg
     1921 ggatcctgag ccgcgccatg cggtgctgga ggaggcacgc aaggtcttgt cacagaagtt
     1981 cccggcgttc gcggggatgc agatcgcgca gagctggggc ggaatgatcg atgtgacgcc
     2041 tgatgcggtg ccggtaattg gggaggtgcc gtcgctacct ggcttctttc tgtcgaccgg
     2101 gttctctggc catggctttg gcattggccc cggcgcaggg cgtctgaccg ccgatctggt
     2161 gtccggcgac acacctatcg tcgatccgca tccgttccgg ttcgggcggt tcagctgagg
     2221 gcgcgactgt cgttgcgagg ggtgaagcga ggaagcaaac tcggcctggt cagagcagcc
     2281 cgtcgagcag gtatcgccac tctgggttca tgccattgat cagcgctgtc ttcctggcgc
     2341 gcgaaccgca cttgatcggt ttttccggta ttatggagca aagaccaact tgccacgaag
     2401 accgcaaggt tcattaaagc cgaactaaag aggtccggcg tgacctacgg cgaactcgcc
     2461 aaacgccttg agtcgcagag cctgaacgaa acagaggcat cgatcacaaa taaactggct
     2521 cgcgcgactg ctcccgctac tcttttgctg gcgactttag cggcgctcgg aatggagggg
     2581 atgaagctgg atgacttgtg agggcgccta cgccaatata ctcgccattc cggtttagct
     2641 gcattggtcc agaccaagct ttcgtaagcc gtgcagagga tggtggttac caagtgccgg
     2701 attggcttgc gtcggttcca gaactgacga acgataagac cattcaacca ggggagggaa
     2761 tgatgccggg ttatgttctg acggagaacg acggcgagca tgtgttccag cgcagtggcg
     2821 agatcgtcat caagatcgat ccgacccgtg gatcgcatga gctatgcctt ggtacacaac
     2881 ggatgccggt aggaattggt gttccccgcc atgtgcatgc ctattgggat gagcacatct
     2941 atgtactgga tggcagtgga tacatcaccc tcgacgatga acgggtgcca atccagaagg
     3001 gcgcgacggt cttcatcccg aagggatgtt ggcatgggtt cgagaaccct gactcggagt
     3061 tattcctcat atgggccgcc gcgccgactg ggcaggagga attcttccgt gcgatatcaa
     3121 gcaagccggg cgaaccaccc aagaatcttt cgcgtgagga agtgttggcg atccgccatc
     3181 aggtcgaggc cgactacctg aagcgggttc aagcgcaggc ttgatgtgat cggttgccga
     3241 tgctcctaac aacgtctcgg cagccgtgac tttggtgttt gcgccataag accgagtttt
     3301 tccctggcga tggccgccag agaatcgcca aattcctcat agtacaccag ctgattgaca
     3361 tcataccgat gggtgaagcc tttgaatccc ggatcccgat gttcggaaac acgacgcagc
     3421 gggtcgtttg tcactccagt ataaagcacc gtgcgccgac tattggttaa gatgtaaacg
     3481 aaataccgat gctgcattcc cacgagattg tttggccggg tcaagcccgg ccaccgcttc
     3541 gctcctcgca atgacacaaa ccgcgatcct caccaaaccg gccacgatcc cgctcgctgt
     3601 gcgtcagcgc gtactgttcg gcatcctctg cttcgtctgg ggcactacct ggctgggcat
     3661 gaaggtcggc atcgccgttg taccgcccgg cgtcttttcc gggacgcgat ggacaatggc
     3721 cggcctgacg ctgctgctct ggcgctgggc gcgcgggcaa cgcctgctga tcccgatgcg
     3781 actcaccggc aggctgctca tcgttgccgt cctgatggtg tcgctcaatc aggtgatcca
     3841 gctctacggc ctgcgccaca tcacggcagg tcttgcatgt gtgctttcct cggcgctcac
     3901 gccaatcgcg ctgcttggct tcgcggttgc ctgggggcag gaacgtttcg cctggcggca
     3961 gctcgtcgcg ctggggatcg gcatcgccgg tgtgcttgtg ctgttcggac ccgccgcagt
     4021 cgccggcaag ctcgatgcct gggaggtgct cggcgcggcc ggcgtcatca tcggcacgat
     4081 gtgctactcg gccggctccg tcatggcgcg gccattgatg aactcgctcg caccggcgca
     4141 gatggccgcg atgaccaacc tgatcggcgg cgtcctgctg ctgatctcgt ccatcgtgtt
     4201 cgagccaggc gctgcgtccg cgttgatcca cccttggggc tgggcagcct cgattgcctg
     4261 gctttatctc ctgatcccgg gatcgctgct ttccacggtc atttactttc tgctggtccg
     4321 tgattggggt gccagccgta ccggcactta tgccttcgtc tcaccggtgg tcgccgtcgt
     4381 catcggcacc tcgctgttcg gcgagaagct cgatcttggc gacgtcgtcg gcatggtgct
     4441 gatgctcgtc gcggccgcca tcgtgctgcg gcgccctatc gtcccttgaa cctgggcttg
     4501 cgccgttcgc gccaggcctg gtgcgcttca tgcttgtcct ccgtcagttc cagcatggcg
     4561 aagcgataca gatcgccgag tactgcatcg gccagaggca catcggcgcc gcgattgagc
     4621 gactccttcg tcaaccgtgt ggcgagtggc ggcagcgagg cgatgtgctc ccccacctcc
     4681 attgcctgcg ccatcaatgc atcatgcggc accagccact gcgccaggcc gatgcggtat
     4741 gcctcgtccg ccttcaacgg gaagccaagt gcaaggcgca tcgccagtcc cttgccgacc
     4801 cagcgggcca gtcgtgtcgc tccgccctgc gccggaatga tgccgagccc actctgcggc
     4861 agccgccatt ccgcgcgttc cgatgcaacg atcatatcgc agcaataggt gacgatgcag
     4921 ccgataccga cagcgtaacc attgactgcg gcgatgatcg gcttggcgaa gtcggtcaac
     4981 gccgggatcg gatgggcgcg ccgccggttc ggcaggtcct ggatgaatgc ggccacggaa
     5041 tccatcgtgt gcgtgcgggg gtttttcaaa tcggcaccgg cactgaacgc gccgcccttg
     5101 tcatcgccgg tcaggatcac gcagcggacg tcgggatctg cttccagttc cggtaacagt
     5161 tgcacaaggc cggtctgaaa ttcctcgcac cacgcattcc gcgcctcggg gcggctgagc
     5221 gtcaggacgg cgatggtgcc gtggcgatcg cagagcaggg gcatcggcgg ttcctccttg
     5281 gcgccatgct atgcagcagg gtgcaaaaag accatcggag gaaccgtgtc ggcgctacgg
     5341 cgtgtccagc agcagcggtt cgcgcgagac gagcacttcg ccacctgacg tgagacggat
     5401 cacgttgacc gtttgcgcca ggtcgggcga accggagtag taggctggca cgctgccatc
     5461 gccgacctga gcggggcgat cccaatggcc gagcgccaga tagtcagcct gcgtctcggc
     5521 aatgtgcgta tcggtgaaca gccatgatgg gcgcaacggg gtggcgaggt tgtggggcgg
     5581 ctcgtagtgc ccgtgcgcca tcgcaatctg ccagcgagtg gtgcgcgcac gaggtccacg
     5641 cagcggcgcc atgttctcgt agtcgcgatg cgcgtggccc cagatttcca gatcgtgcgc
     5701 ctggaaagcc gtggcttcct cgtgcgtgac gccgaaaatc gacacgcctt ggatcgagcc
     5761 aagcccgcca cgctggaaca cgctgttcgg cattgccgga tcgtggttgc cggggaggat
     5821 caccaccggc atgccggcat cggacagcag acgtcccacg cggtccagca ccggcacggg
     5881 aacgcggttg ttgtcgaaag tatcgccggc aagcagtacg atatcggcat tatgcgccct
     5941 cgcggcggcg agtacagcgg cgagaccggc ggtgccatcg tggtgcttgg tggcggaggt
     6001 ttcatcatcg acgtgcacgt cggagctgtg gacgaggacg atgtccactc gggacggatc
     6061 gcattgcggc atgggctcgc agattacggc gtggtgtgaa agaggcaagg gcgtgcctgc
     6121 gaatgctagc ctcccgcttg cgcccagcgt gccatctgcc cgcgcaccca atcgcctccc
     6181 aatgccaggt ccggctcgaa cgcccgcagc acgggggcca tgatctgcgc caccatttcg
     6241 ggccacgcct ggccgaggct cgcggccggc atctgcgtcg tcaccacccg gtggtcatcg
     6301 ccgaccgcgc ctcgctgcac ccagctcgca tgaggatcga acacctcgcg tcccgccagc
     6361 ccccaccatt cgcagcggaa atgcacccgc cgcacgctgg caaagaaccg ggcaagcgac
     6421 cgggcgtggc agaccagagc cgctacgtcc tgggccagta cattcggact gaaccatgtg
     6481 cctggctgca tgccgagtgc ttcattggcg tcggtcttgt cctcgggata atcgcgcaca
     6541 atgctggcca gcccgcgcgg cgacaggcgc cagaaatccg cggtctcgcc tggcgggcgt
     6601 tcgcgcagcc acgcaacctc gaggaaatcg gtttcgttgt cgccggtcgc gtcatcgacc
     6661 acgaaccggg gctgtaccga acggcggtag ggcgggtcga acagcatcat cgaatcgcgg
     6721 aacagcggcc tcagctcgaa gacgacgcgg cgcaggatat caggaagctg cgcctggtcc
     6781 ggcaccgccg gtcccgccag ctcgaacgca tacgaaagca tgtagtgtgc gcgtccaagc
     6841 tgctcagcga ccgggctcag tggcacaagc cgcaggaagg ccgcatgcgc ggccgcgtgc
     6901 cagatggcga ggcgttcgac gacgtccggc acgttcgccc gcccttcgac cgcggcaccg
     6961 atcatcgcca gcagtgcctc gcggtcctgc cgcacgcatc gacggatcag cggcgcccag
     7021 tcctgaggcg tctcgatccg ctcggtccgt ggccgcggaa tgccgatgaa tgcgcccgcc
     7081 acggcgccat gcttgcggat atagaagctg cctcgctcga ggagccgcga cggtccgacc
     7141 agcggtccgt cctggcgcac gcatatgggc accggaccgt gacccgccac ccggattgcc
     7201 ggatgcgccg aaccggtggc ggagcgcagc gtcgcgacct cgcaggcgac cggcgggtcc
     7261 aggtaggtgc ggacgatggc gctcacctgt tcgggcgagc aacgcgtcac gaagggatcg
     7321 gtgtcctcgg gtgtcaatgt atcctggtcg aaaccaaaga tgatgtgccc gccaccgtgg
     7381 ttagcgagtg cggcgatgtc ccgtgcgagt tcggcacgat cctcgccgtt cgacaggttg
     7441 cgccagccct tgtactcaac ctccagcgcg cggcacggtg tatcgacaag tgcctgcaca
     7501 tcattcgggg ttgccggcgc gaagatgccg ctttccagca agggcgacgg cgcgtcctcg
     7561 tcgttggcgg gcagcagatc aggctcggga agcggttcga tggcttcgga cggcatcgct
     7621 gtcggcacaa cggttgttag ttccacttat cactgtaatc actacacggt gctttctgca
     7681 tcagtccaat gcccacctgc gtttgtttgt cgcgagaaaa tgcaaatagg tcacagtgtt
     7741 tcatctgcgc aacggcgacg taagcaatat tgatacgaat agcaaaatcc atcctgtcat
     7801 cagtagtgtc ccaccaattg gtgcgaatag cggcagccgg ataccgtcca gtgcgagcgc
     7861 atatacggcg ccgcagaaca gcacgaggcc aacggtaaat acgccacccg ccgcatccgc
     7921 cagccggccg ccgcgtggtg cccacaaccc gcacgccaac aatgccaggg cgtgccagcc
     7981 gtgcatctgc aaggcgttgc gcagcatctg cagtgatggt ggatcgagcc tgtccaaccc
     8041 gtgcgccgcc agggcggcca tcgcgacagc actcagcccc gccagcgcgc caatgcccat
     8101 ccaaagtcgc tgcattcgga cccctgttcc gcgccggctg cgttgctata ccatcagcga
     8161 ttatgccgcg cggcgacctg tttcctgaga tcggaccatt cgagaccggc tatctgcctc
     8221 tctcgtccaa ccatgtcatg tactgggagc aggtaggcaa tccccgtggc cgcccggtgc
     8281 tgttcctgca tgggggccca ggcgcgggcg cgggcgcagt gcatcgccgc tttttcgatc
     8341 cggaattctg gcgcgtcatc attttcgatc agcgcggggc agggcggtcg cgcccactcg
     8401 gcagcctgga tgacaacacc acccaggatc tcgtctcgga cattgagacg ttgcgtcgcc
     8461 atctcggcct ggagcgctgg ctgctgttcg gcggctcctg gggttccacg ctcgcgcttg
     8521 cctatgccca ggcctgcccg gagcgtgtca tcggctgcgt gctgcgcggg gtgttccttg
     8581 gccgcccggt cgaggtggac tggttcctcg atggcctcgc cgcaatcttt cccgacgcgc
     8641 acgccgcgtt cgtcggcttc ctgccggagg aagagcgcgc tcatgtcctc ggcggctatc
     8701 tgagccggct gaccgatccc gatcccgccg tgcatctgcc ggccgctcgc gcctggtcga
     8761 tctacgaggg ttcctgcagc acgttgctgc ccagccccga gaccgtcacg tccttcgcgc
     8821 aggaccgcac cgcgctgggc ctcgcgcgga tcgaggccta ctactttgcg cacggactgt
     8881 tcctgccgcc cgagggcctg ctgggtaaca tgcaccgcat cggccgcatt cccgccgaga
     8941 tcgtgcaagg gcgctacgac atgatctgtc cggcgcgcac cgcgttcgaa ctggcggctg
     9001 cgtggccggc ggcgcggctg tcgatcgtgc cggatgccgg gcattcggca ctggagccgg
     9061 gcatccgccg cgcactggtc gcggccgtgg agcgcttccg gcaagggagg taatcgccat
     9121 gaaggttgga gtgatcgggc tgggcaccat cggtcttggt gccgcgctga atctcgcgcg
     9181 caaagggcac caggttgtcg gctgcgatgt gcgtgaggcc gccagggccg agatccccac
     9241 ggcggtcgcc cacccggatg agttgcccgc ggacaccgag gcagtggcgg tgttcgtggt
     9301 gaatgcggcg cagaccgaag acgtgctgtt cggggcgcgt gggtgcctgg ccaagctggc
     9361 caagggcgtg gtggtgctga gctgcgccac cattgcgcca gaagcggcgc gcgcgctgga
     9421 tgcgaagatc agcgctgccg gttgcctgat gctcgatgca ccgacctccg gcggcaaggt
     9481 tggcgcgcat ggcggcacca tgacggtgat ggcctctggc gctgaggctg cgttcgccaa
     9541 ggcgcagccg gtgctggatg cgatttccac caaggtctgg cggctcggcg acgctgtcgg
     9601 cgtcggcagc acggtgaaga tggtcaacca gttgcttgcc ggcgtgcata ttgcgacggc
     9661 ggcggaggcg atggcgctcg gcatccgcgc cggggcggat ccgcgcacgc tgttcgaggt
     9721 gatctcgtcg tcggccggca gttcctggat gtggcagaac cggatgccgc acgtgctgga
     9781 aggcgatgac accccgctgt cggcggtgaa catcttcgtc aaggatcttg gcatcgtgct
     9841 ggatcaggcg cgggcgctga ccttcccgct gccgatggcg tccgccgcgc atcagttgtt
     9901 cctggcggcc gccgcgcatg gcgaaggcgc aaaggacgat gctttcgtga tccgcgtctg
     9961 gcaggcgctt gcggggatcg atctgccgaa gggcggataa tcggcgcatt gccttgaatt
    10021 ggcggtggcc gccggtcatc atcgcaggca atggctgatc cacagaaacc cggccaacgc
    10081 ctcgactacc aggccagtat cgatcgtccg cgtctcgcgt tgccggacgg caagcgcgtc
    10141 gccgtgttca ttgtggtgaa cgtcgagaac tgggacatcg atcgcgccat gccgcggcag
    10201 gtgctgacag cgccgcaggg cgcctcggtg gtccctgatc tgccaaactg ggcctggcat
    10261 gaatacggca tgcgcgtcgg catctggcgc atcaaggccg cactcgatga gcatggcatc
    10321 gcgccatcga tgacgatcaa cggcactgtg ccggcggcct acccgcgtgt cgcacgtgcc
    10381 gcgcgcgatg ccggctggga gttcatgggc catggcttcc ggcagatggc gacacatctg
    10441 gtggatgatc agcgcgccat gatccgcgat gcggtcagcg cgctgcgcga ggctgcgggg
    10501 cacgatatcg tcggctggct ggcacctggc ctgaccgaga ccctggatac gcccgatctg
    10561 ctcgcggaag ccggcatccg ctactgcgcg gactgggtga tcgacgacct tccgtgcacg
    10621 ctgcgcacag cgcatggcct gatcctgacc atgccgtaca gcgtcgaact gaacgacatt
    10681 ccgatgatga tgatccagca tcatcgcgcc gaggaacttt ttgcgcgcac ggtggcgcag
    10741 ttcaatcgcc tctatgccga ggcagagacg caaggcgcga aggtgatggg cctcgcggtg
    10801 catccgtata tcagcggcgt gccgcatcgc atcggctggt tcgagcaggc gttgagctac
    10861 atggcctcga agccgggcac cttgttctgg cagggacggc agatcatgga ctggtatctg
    10921 aaggcggcgg catgaactgg ggagcgatga gccgcgcgga gcgcgacgcg gcctacaaca
    10981 actcggaggc ggtgaagaac agcccgcaac tgaatgccgc acgcgccgca gcatcggccg
    11041 aattccgtgc ggcgcatccg gggcatctgg atcttcctta cggtccgcgt gaacggaaca
    11101 cgtgggacct gtttcccgcc aaggatccga atgcgccgtg cattgtgttc atccacggcg
    11161 ggtactggca gcgcaacagc aaggaccagt tcgccaacct gatcgccggt ccgtacgcgc
    11221 acggctgggc gggcgcgctg ccaggctaca cgctgggtcc tgatgcgacg ctcaccgaga
    11281 tcaccgccga gatcaatgcg gcgctcgatt ggctggtggc gaatgggcct tcgcatggca
    11341 tcagcggacc gatcgtgctg tccggctggt cagccggcgg gcatct
//
LOCUS       DATGEM010000170         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_524094, whole genome shotgun sequence.
ACCESSION   DATGEM010000170 DATGEM010000000
VERSION     DATGEM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524094"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1265)
                     /locus_tag="VKQ27_05350"
     CDS             complement(90..1265)
                     /locus_tag="VKQ27_05350"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HLY88390.1"
                     /translation="MSSTTPYGAVALIVAGGNGTRAGGDIPKQYRRIGGKAVIARAFD
                     ALFAHPAIDAVFVVIGDGQESLLSAALGGRMPAKVVRGGATRRESVSAGLEAIDAAGG
                     ARSVLIHDAARPFLPDTVIDRLLTAIEVHPGAVPTLPVVDTLVRTDDSQLGDTVSREG
                     LARVQTPQAFRFDTIVAAHRGWSGPQEPTDDAQVARHAGLAVIAVPGDSMLEKLTHEA
                     DLTAAETRLATTLVSRTGSGFDVHAFAEGDHVMLCGLKIPHSHALAGHSDADVGLHAI
                     TDAVLGAIADGDIGSHFPPSDPRWKGADSGKFLQHAASLVAAKGGIIDHVDLTIICEA
                     PKVGPHREAMRQRIADLLALPLGRVSVKATTTEKLGFTGRGEGMAAQAMATVRVPDA"
     gene            1429..2394
                     /gene="dusB"
                     /locus_tag="VKQ27_05355"
     CDS             1429..2394
                     /gene="dusB"
                     /locus_tag="VKQ27_05355"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HLY88391.1"
                     /translation="MTIDTPVILAPMTGVSDLPFRKMVRRYGSGLNVTEMIASQAAIR
                     ETRQSIQKAAWDPIEEPVSMQLVGCSPAEMGEAAKLNEDRGAAIIDINMGCPVRKVVN
                     GDAGSALMRDLELAASLIRATVDAVTVPVTLKMRMGWDLDSLNAPELACIAEGLGVKM
                     VTVHGRTRNQMYKGSADWAFIRRVKDAVSIPVIANGDICSVEDAEEALLQSGADGVMI
                     GRGAYGRPWLLGQVMRYLTTGERRDDPSIDEQYAVIVEHYEMMLGHYGKETGVNMARK
                     HLGWYTKGLPGSAEFRNVVNQVPDSDRVLAMLDGFYAPWRSRAAA"
     gene            2370..3491
                     /locus_tag="VKQ27_05360"
     CDS             2370..3491
                     /locus_tag="VKQ27_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY88392.1"
                     /translation="MALPGSRLIRFSRRGSGDAEAPLPAELVAALPTALLVIDGDRCV
                     RDCNSAAEALLNLARSALLGRSIEDLVGHPLRSMAADQPFAAYDLDMILPMARPYHGD
                     MTVAPLPEWAGWRIVTIHGRASHDLAARRSERTGRTLPAAAAASLLAHEIKNPLSGIR
                     GAAQLLESDASPEQVELTRLIRGEVDRIAGLIDRMEGLSDTRPRPREPLNIHSVLGHA
                     RDVARAGFARGRTIREFYDPSLPDVLGNRDGLIQILLNLLKNAVEATNETGVITLTTA
                     YRQGLSIEAPGNGGRMALPIELCVIDDGPGAPEVLVPHLFDPFVTSKPSGRGLGLALV
                     DKLVTEMGGVVEYAREGSPERTLFRLLLPRAPSRRELTS"
     gene            3488..>4235
                     /locus_tag="VKQ27_05365"
     CDS             3488..>4235
                     /locus_tag="VKQ27_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLY88393.1"
                     /translation="MNAILTGRVLVVDDDAAIRTVVREALRRAGHLVEAVGSIADLRH
                     AIGTFAPDVLITDVMLPDGDGLDLVPELLERHPDLPIIVLSARNTLATAVRATEQGAF
                     DYLPKPFDLEELGQAVSSALAGRMADTDVAGESANHELPLIGRSPTMQTVYRTIARVV
                     SNDLTVLILGESGTGKELVARAVHDLGPRAARPFVPVNMAAIPRELIESELFGHERGA
                     FTGAAQRTAGRFEQAAGGTLFLDEIGDMPLE"
BASE COUNT          736 a         1366 c         1412 g          721 t
ORIGIN      
        1 cgcgcgcatg tcgcgaggct ccacgcaggc cgggcgaact gcctcggcct gcccggcgca
       61 gagcgcgaga agggcgagcg ccgcccgttt catgcgtcag gcacccgcac cgtggccatc
      121 gcctgtgcgg ccatgccctc accgcggccg gtgaagccaa gcttctccgt ggtggtagcc
      181 ttcacactga cacggcccag cgggagcgcg agcagatctg cgatccgctg gcgcatcgcc
      241 tcgcgatgag ggccgacctt gggtgcctcg cagatgatgg tgaggtcgac atgatcgatg
      301 atcccgccct ttgccgcgac gagactggcg gcgtgctgga ggaacttgcc cgaatccgcc
      361 cccttccatc gtgggtcgga cggcgggaag tggctaccaa tatctccatc cgcgatcgcg
      421 cccagcaccg catcggtgat ggcatggaga ccgacatccg catcactgtg tccggccagc
      481 gcatggctgt gggggatttt gaggccacat aacatgacat gatcgccctc ggcgaaggcg
      541 tggacatcga accccgaacc ggtgcgcgac accaatgtcg tggcgagtcg ggtttcggcg
      601 gctgtgagat cagcctcgtg tgtaagcttt tcgagcatgg aatctccggg gacagcgatg
      661 acagcaaggc ctgcgtggcg cgccacctgg gcatcatcgg taggctcctg cggccctgac
      721 cagccgcgat gcgcggcgac gatggtgtca aagcggaagg cctgcggagt ttgcacgcgg
      781 gccagcccct cgcgggagac agtgtcgccg agttgactgt cgtccgtccg aaccagcgta
      841 tcaacgacgg ggagcgtcgg gacggcgccc ggatgaactt cgatcgcggt caggagtcga
      901 tcgatcaccg tatccggcag aaatgggcgt gcagcatcat ggatcagcac ggatcgcgca
      961 ccgcccgccg catcgatcgc ttcgaggcca gccgaaaccg actcgcgtcg cgtcgcacca
     1021 ccacgaacca ccttggcagg catccgtcca cccagtgccg cggagagaag tgattcctgg
     1081 ccgtcgccga tcacaacgaa gaccgcatcg atggctggat gcgcgaaaag cgcatcgaac
     1141 gcccgcgcga tcaccgcctt gccaccgatt cgccgatatt gtttgggaat atcgccgccg
     1201 gctcgcgtgc cgtttcctcc ggcgacgatc agcgcgaccg cgccatatgg tgtcgtggaa
     1261 ctcatggggc atgcgcctaa cccctgaaag cgcgagtgtg aagcggccgc gcgcatatgc
     1321 gtgcacgctt gccgcaacgc cgcaaaaagg gtagggcgat tgccttcttt ttaggcagat
     1381 gaggatatgc gggacgaacc gctcgtgaag ccgatcaagg tcggcccggt gaccatcgac
     1441 acgccagtga ttctggcgcc gatgacgggc gtcagcgatc tgccgttccg aaagatggtg
     1501 cgccgctacg gctcggggct caacgtcacc gagatgatcg ccagccaggc tgcgatccgc
     1561 gagacgcgtc agtcgatcca gaaggcggcg tgggatccga tcgaggagcc ggtgtcgatg
     1621 cagctcgtcg gctgttcgcc ggccgagatg ggcgaggcgg ccaagctcaa cgaagaccgc
     1681 ggcgctgcga tcatcgacat caacatgggc tgccccgtcc gcaaggtcgt gaacggcgat
     1741 gcgggctcgg cgctgatgcg cgatctcgaa ctggcggctt cgctgatccg agcgaccgtg
     1801 gacgccgtca cggtgccggt cacgctcaag atgcggatgg gctgggatct ggacagcctc
     1861 aacgcgcccg aactggcatg catcgcagaa gggctgggcg tgaagatggt cacggttcat
     1921 ggccgtaccc gcaaccagat gtacaagggg tcagccgact gggcgttcat ccggcgcgtg
     1981 aaggacgcgg tgtcgatccc ggtgatcgcc aatggcgaca tctgctcggt cgaggatgcc
     2041 gaagaagcgc tgctgcaatc cggggcggac ggcgtgatga ttggccgtgg cgcctatggc
     2101 cggccctggc tgctgggcca ggtgatgcgc tacctcacca ctggcgagcg ccgcgacgac
     2161 ccttcgatcg acgaacaata tgccgtgatc gtcgagcatt acgagatgat gctcggtcat
     2221 tatggcaagg agacgggcgt caacatggcc cgcaagcatc tgggttggta caccaagggc
     2281 ctgccgggat cggccgagtt ccgcaatgtg gtcaaccagg ttcccgattc tgaccgggtg
     2341 ctggcgatgc ttgacggctt ctatgcgcca tggcgctccc gggcagccgc ctgatccgct
     2401 tcagtcgccg tggatcaggg gatgcggagg cgccgcttcc cgccgagctt gtcgctgcgc
     2461 ttcccaccgc actgctcgtc atcgatggtg accgctgcgt gcgggattgc aattcggcag
     2521 ccgaggcgct gctgaacctc gcacgatcgg ccttactggg gcgttcgatc gaggatctcg
     2581 tcgggcaccc gttgcgatcg atggcggcgg accagccctt cgcggcctat gatctggaca
     2641 tgattctgcc catggcgcgg ccctatcacg gtgacatgac ggtggcccct ctgcccgaat
     2701 gggcaggctg gcggatcgtc acaatccatg gccgcgccag ccatgatctc gccgctcgcc
     2761 ggagcgagcg caccggccgg acactgcccg cggcggcggc ggcctcgctg ctggcgcacg
     2821 agatcaagaa cccgttgtcc ggtatccgtg gggcggcgca gctcttggag agcgatgctt
     2881 cgcccgagca ggtcgaactg acccggctga tccgcggcga ggtcgatcgc atcgccgggt
     2941 tgatcgatcg catggagggg ctctcggaca cgcgcccgcg cccgcgcgag cctctcaaca
     3001 tccattcggt gctgggccat gcccgcgatg tcgcgcgtgc gggcttcgcg agggggcgca
     3061 cgatccgcga attctacgat ccctcgctgc ccgacgtcct tgggaatagg gacggattga
     3121 tccagattct tctcaatctg ctcaagaatg ccgtggaagc gacgaatgag acgggagtaa
     3181 tcaccctcac gaccgcctat cgccaaggcc tctcgatcga ggcgcccgga aacggtggtc
     3241 gcatggcgtt accgatcgag ctgtgcgtga tcgacgacgg ccccggagct cccgaagtcc
     3301 tcgttccgca cctgttcgat ccgttcgtga cctccaaacc ctccggccgc ggcctcggcc
     3361 tcgcgctggt cgacaagctg gtgacggaga tgggaggcgt tgtcgaatat gcgcgcgagg
     3421 gctcgccgga acgaacgctc tttcggctcc tgctgccccg tgcgccatcc cgtcgggagt
     3481 tgacgtcatg aatgcgatac tgaccggccg tgtcctggtc gtcgacgacg acgcggcgat
     3541 ccgcacagtc gttcgcgaag cgctgcgccg tgccgggcac ttggtagaag cggtgggcag
     3601 catcgccgat ttgcggcacg cgatcgggac gttcgccccc gatgtgctga tcaccgatgt
     3661 gatgcttccc gatggagacg ggcttgatct cgtcccggaa ctgctggagc gccatccgga
     3721 cctgccgatc attgtcctct cggcccggaa cacgctggcg actgcggtgc gcgcaaccga
     3781 acagggtgcg ttcgactatc ttcccaagcc tttcgatctc gaggaactcg gacaagccgt
     3841 gtccagcgcg cttgccggcc gcatggccga cacggacgtg gcgggagaga gcgccaacca
     3901 cgaactcccc ctgatcggcc gctcgccgac gatgcagaca gtctatcgca cgatcgcgag
     3961 ggtggtctcc aacgacctca ccgtgctgat cctcggagaa tcaggaaccg gcaaagaact
     4021 ggtggcgcgg gccgtccacg acctcggccc acgcgcagcg cgtccgttcg tgccggtcaa
     4081 catggcggcg atcccgcgcg aactgatcga aagcgaactc ttcggccacg aacgcggcgc
     4141 gttcacgggg gccgcccaac gcaccgccgg ccgcttcgag caggctgcgg gcgggacgct
     4201 gtttctcgat gagatcggcg acatgccgtt ggagg
//
LOCUS       DATGEM010000171         5204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_673490, whole genome shotgun sequence.
ACCESSION   DATGEM010000171 DATGEM010000000
VERSION     DATGEM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673490"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VKQ27_05370"
     CDS             complement(<1..416)
                     /locus_tag="VKQ27_05370"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLY88394.1"
                     /translation="MPNRLVFPGGAVDRADLGAPAATPLTAQTEHLLRKNANERLAHG
                     LGIAAARELHEETGLTLGTPPRLDALHLLARAVTPPLSPIRFNARFFVVDAVHVAGSL
                     GGDGELEGLRFYGMQEALSLDLAMPTRRVLERLHVW"
     gene            complement(571..1395)
                     /locus_tag="VKQ27_05375"
     CDS             complement(571..1395)
                     /locus_tag="VKQ27_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLY88395.1"
                     /translation="MADSAELLRARFGIDLPSVEVPPEIAHLVDRRVTRRYTEQPVPD
                     SLLDALLAAAQSAPTKSDLQQYSVVVMRDRARIKQIADWIGTMDWIATAPVFLVWCGD
                     MRRNQRLCEQHGMPHANNNLDTFLNTAVDCTLAMGQFIVAAEAIGLGTCPISYVRSHI
                     ERVTPLLSLPPGVYPVAGLSIGWPVFRRPIAMRLPPSVVVHRERYDDSNLKDHVGAYD
                     DRRRAREPVAPGSLKNNDVYPPREGVGWSENVARQLSVPERFGFAAYLKTRGFDLA"
     gene            1472..2536
                     /locus_tag="VKQ27_05380"
     CDS             1472..2536
                     /locus_tag="VKQ27_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HLY88396.1"
                     /translation="MPAPYATHRAQIKAILLAWGMPEENAEPTADILSWADLHGVDSH
                     GMSMLPGYDRLRRTGRAKMDVRPAIVKETAISALVDGGGGLGHVPAHFAMQVAIDKAK
                     ASGMAIAVVKNSAHFGATGYYTLMAAKQGLVGMACTSASGIQVAPTFGKQAKLGTDPW
                     SFAAPSADGVPFLLDMATTTVAAGRIRNKANEGLQCPPGWVLNKEGLPSTDPLEAREK
                     GGFLTSLGGSPENSSYKGYGLAAMVNILASCLSGATLITDPQHTKKPQGMDIGHFFMA
                     IDPELFRTSEEFQADVAQFCGDLRATKPVDPAQPVMVAGDPQWKNAENRMRDGVPVGP
                     GLLNQVRQIAQASAAPWLLD"
     gene            complement(2540..4417)
                     /gene="cobT"
                     /locus_tag="VKQ27_05385"
     CDS             complement(2540..4417)
                     /gene="cobT"
                     /locus_tag="VKQ27_05385"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HLY88397.1"
                     /translation="MSATNKDNTRAEEFKRATAGVLRAIAEVPDVQVAFQPGPSGLSG
                     KRARLPLPTRALPPQEMARLRGASDALALRLRHHDDTVHASRMPARREAKDVYDALEQ
                     VRVEVVGARHMAGVAANLQSKLAEECEAEGYDRMTRKDQLPVQAALSLLAREQMSGEK
                     SPPAAQRILDLWRDTLGDNAESALAEMNAAQDDQTAYARAARKLLAALDLAEAESDAE
                     PEDNTDEGEEGGEQSGQQDEQQDGSGQSEAEQDSMLGANPEEMEGDAADDEGTESDEE
                     AAIAEGDDRPGGPQPRRERPNPDNEAVYRAYTRLYDEEIDAEELCDPDELTRLRQQLD
                     QQLQHLQGVISKLANRLQRRLLAQQTRAWEFDLDEGLLDAGRLSRVVVNPLQALSYKR
                     ELDMEFRDTVVTLLIDNSGSMRGRPITVAAMCGDILARTLERCAVKVEVLGFTTRAWK
                     GGQSREKWVQEGKPRNPGRLNDLRHIIYKAADAPWRRARKNLGLMLREGLLKENIDGE
                     ALLWAYRRLLVRAEHRRILMVISDGAPVDDSTLSVNPGNYLEKHLREVIRDIEGRDQV
                     ELIAIGIGHDVTRYYRRAVTIVDAEELGGTMMKKLTELFDEDMDAAWARAAGERAAIV
                     V"
     gene            complement(4525..>5204)
                     /locus_tag="VKQ27_05390"
     CDS             complement(4525..>5204)
                     /locus_tag="VKQ27_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HLY88398.1"
                     /translation="LIGKDAIVLKDGKQITEFREGILPWALQHACALVFDEYDAGRPD
                     VMFVIQRVLEVEGKLTLLDQNRVIRPHPSFRLFSTANTVGLGDTTGLYHGTQQINQGQ
                     MDRWNIVATLNYLPHAMETSIVLAKLGADTKDATMKKRIEAMVALADLTRAGFINGDI
                     STVMSPRTVITWAENARIFGDVGFAFRVTFLNKCDEAERSTVGEYYQRCFNEEIPTGG
                     FTTRKFN"
BASE COUNT          842 a         1567 c         1824 g          971 t
ORIGIN      
        1 aaccacacgt gtagccgctc cagcacgcgt cgcgtcggca tggccagatc gagcgagagc
       61 gcttcctgca tgccgtagaa gcgcagtccc tccagttcgc cgtcaccacc caggctcccg
      121 gccacgtgca ccgcatcgac cacaaagaag cgtgcattga agcggatggg actgagtggc
      181 ggggtgacgg cgcgtgccaa cagatgcagc gcatcgaggc gtggcggcgt gccaagtgtc
      241 aggcccgttt cctcgtgcag ttcgcgcgcc gcggcgatac cgaggccgtg cgcgaggcgc
      301 tcattggcat tcttgcgcaa cagatgttcg gtctgggcgg tgagcggggt cgcggcgggt
      361 gcgccaaggt cggcgcgatc caccgcgcca ccagggaaca cgaggcggtt tggcatgaag
      421 cgatgtttgg ccccgcgcat gcccattagc attgaaatgt cgccgccgtc ccggcggtac
      481 acgacgaggc tggcagcatg gcgcggccgc accgcgcgag ggcgaggtgg cgtctgctgg
      541 tcaatgagcg gtgcgtgacg gacatggtct tcaggcaagg tcgaaccctc gcgtcttcag
      601 gtaggcggca aagccgaaac gctccggcac cgaaagctgg cgggcgacat tctcgctcca
      661 gccaacgcct tcgcgtgggg gataaacgtc gttgttcttg aggctgccgg gagcgaccgg
      721 ttcgcgggca cggcgacgat catcgtaggc gccgacgtgg tccttgagat tcgagtcgtc
      781 gtagcgttcg cgatgaacca cgacggatgg aggtagccgc atggcgatgg ggcggcggaa
      841 cacgggccag ccgatcgaga gtccggcgac gggatagaca ccggggggca gcgagagcag
      901 gggcgtgacg cgctcgatat ggctgcggac atagctgatg ggacacgtgc cgaggccgat
      961 cgcctcggcc gcaacgatga actggcccat ggcgagcgtg caatcgaccg cggtgttgag
     1021 aaaggtgtcg agattgttgt tggcatgcgg catgccatgc tgttcgcaca ggcgctggtt
     1081 ccgccgcatg tcgccgcacc acaccaggaa gactggcgca gtggcgatcc agtccatcgt
     1141 gccgatccag tcggcgatct gcttgatacg cgcgcggtcg cgcatgacca cgacggaata
     1201 ctgctggagg tcagacttgg taggcgcgga ttgagcggcg gcaaggaggg cgtcaagcag
     1261 cgagtcgggg accggctgtt cagtataccg gcgagtgacg cggcgatcga cgaggtgggc
     1321 gatttccggc ggcacctcga cgcttggcag gtcgatgccg aagcgtgctc gcaggagttc
     1381 tgcactgtct gccatttcat acctccgcag ggcacacttg ccggaacagc gtgcccgcgc
     1441 aaggttgtcg ctacccacgg aggagacgaa catgcctgcc ccttacgcaa cgcatcgtgc
     1501 acagatcaaa gccattctgc ttgcctgggg catgccggaa gagaacgcgg agcctaccgc
     1561 cgacattcta tcgtgggccg atctgcatgg tgtggacagc cacggcatgt cgatgcttcc
     1621 cggatatgac cggctgcgcc gcacgggacg cgcgaagatg gacgtgcggc ccgcgatcgt
     1681 gaaggagacc gcgatctcgg cgctggtgga tggcggtggt gggctggggc acgtgccggc
     1741 gcatttcgcg atgcaggtgg cgatcgacaa ggcgaaggcg tcggggatgg cgatcgccgt
     1801 ggtcaagaac tcagcgcatt ttggcgcgac cggttattac acgttgatgg cagccaagca
     1861 ggggctggtc ggcatggcct gcacatctgc ctcgggcatt caggttgcac cgacgttcgg
     1921 gaaacaggca aaattgggga cggatccgtg gtcgttcgcc gccccttcgg cggatggcgt
     1981 gccgttcctg ttggacatgg cgaccacgac ggtggcggcg gggcggatcc gcaacaaggc
     2041 gaatgaggga ctgcagtgcc cgcctggctg ggtgctgaac aaggagggac tgccgagcac
     2101 cgatccgctg gaggcgcggg agaaaggcgg cttccttacg tcgctcggtg ggtcgccgga
     2161 gaattctagc tacaaagggt acgggctcgc cgcgatggtg aatatcctcg cgtcatgtct
     2221 gtccggcgcc acgctcatta ccgatccgca gcataccaag aagccgcagg ggatggatat
     2281 cgggcacttc ttcatggcga tcgatccgga attgtttcgg acctctgagg agttccaggc
     2341 cgacgttgcg cagttctgtg gcgacctgcg ggcaacaaag ccggtggacc cggcgcagcc
     2401 ggtgatggtc gcaggcgatc cccaatggaa gaatgccgaa aatcggatgc gcgatggtgt
     2461 gcccgttggg cctgggctgc tcaaccaggt gcggcagatc gcacaggcgt ctgcggcacc
     2521 ttggttgctg gattaggcgc tataccacaa tcgccgcgcg ctctccggcc gcgcgcgccc
     2581 acgcggcgtc catgtcctcg tcgaacaatt cggtaagctt cttcatcatc gtgccgccca
     2641 gctcttcggc atccacgatg gtgaccgcac ggcggtagta gcgggtcacg tcatggccga
     2701 tgccgatggc gatcagttca acctgatcgc ggccttcgat gtctcggatg acttcgcgca
     2761 ggtgcttttc caggtagttg cctgggttca ccgacagcgt cgaatcatcg accggtgcgc
     2821 cgtcgctgat caccatcagg atgcggcgat gctcggcgcg caccagcaat cggcgatacg
     2881 cccagagcag ggcctcgccg tcgatgttct ccttcagcag gccctcgcgc agcatcaggc
     2941 cgaggttctt gcgggcgcga cgccagggcg cgtcggcggc cttgtagatg atgtggcgca
     3001 gatcgttcag gcggcccggg ttgcgcggct tgccttcctg tacccatttc tcccggctct
     3061 ggccgccttt ccaggcgcgc gtagtgaagc cgaggacctc caccttcacc gcgcagcgtt
     3121 ccagcgtgcg cgccaggatg tcgccgcaca tcgccgcgac agtgatcggg cggccgcgca
     3181 tggagccgga gttgtcgatc agcagggtca caacagtgtc gcggaactcc atgtcgagtt
     3241 cgcgcttgta ggagagcgcc tggagcgggt tgaccaccac acgtgacagg cgtccggcgt
     3301 cgagcaggcc ctcgtccagg tcgaactccc aggcgcgggt ctgctgcgcg agaaggcggc
     3361 gctgcagccg gttggcgagc ttcgagatca cgccttgcag gtgctggagt tgctggtcga
     3421 gctgctggcg cagccgggtc agttcgtcgg gatcgcagag ctcttccgcg tcgatttcct
     3481 cgtcgtatag acgggtgtag gcacggtaga cggcctcgtt gtcggggttg gggcgttcgc
     3541 ggcgcggctg cgggccaccg gggcggtcgt cgccctcggc aatcgctgct tcctcatcgg
     3601 actcggtgcc ttcgtcgtcg gctgcgtcgc cttccatttc ctcggggttg gcgccgagca
     3661 tgctgtcctg ctcggcttcg ctctggccgg agccgtcctg ctgttcgtcc tgctggccgg
     3721 attgctcgcc gccttcctcg ccttcatcgg tgttgtcttc gggttcggcg tcgctttcgg
     3781 cttccgccag gtcgagagcg gccagaagct tgcgggcagc tcgggcatag gcggtctgat
     3841 cgtcctgcgc agcattcatc tcggcgaggg cggattcggc gttgtcgccg agtgtgtcac
     3901 gccagaggtc gaggatgcgt tgcgcggcgg ggggagattt ctcaccagac atctgctcgc
     3961 gggcgagcag ggagagagcg gcctggaccg ggagctgatc cttgcgggtc atgcggtcgt
     4021 agccttccgc ttcgcattct tccgccagct tggattgcag gttcgcggcg acgccggcca
     4081 tgtggcgagc gccaacgact tcgacacgga cctgttccag ggcgtcgtag acgtccttcg
     4141 cttcgcggcg cgcgggcatc cgggaagcgt gcaccgtatc gtcgtggtgg cggaggcgaa
     4201 gggcgagcgc gtcgctggcg ccgcgaaggc gggccatttc ctgggggggc agggcgcggg
     4261 tcggcaaagg cagccgggcg cgcttgccag agaggccgga ggggcctggt tggaaggcga
     4321 cttggacgtc cggtacctcg gcgatcgcgc gcagcacgcc ggccgtggcg cgtttgaatt
     4381 cttcggcgcg ggtgttgtct ttgttggtgg cgctcattgc gatggttccg cagctccctc
     4441 ccccaacccc caacctctgg ccttggtccg ctacgcggtt caaggccgca aggggcaggg
     4501 gagtgtcggg gcggggctca gtccttagtt gaatttgcga gtggtgaagc cacccgtcgg
     4561 gatctcttcg ttgaagcagc gttggtagta ctcacccacc gtcgagcgct cggcttcgtc
     4621 gcacttgttc aggaacgtga cgcggaatgc gaagcccacg tcaccgaaaa tgcgagcgtt
     4681 ttcggcccag gtgatcacgg tgcgcggcga catcaccgtc gagatgtcgc cgttgatgaa
     4741 gccagcgcgg gttaggtcgg cgagcgcgac catcgcctcg atgcgcttct tcatcgtcgc
     4801 gtccttggtg tcggcaccaa gcttcgccaa cacaatcgag gtctccatcg cgtgcggcag
     4861 atagttcagc gtggcgacga tattccagcg gtccatctgc ccttggttga tctgctgcgt
     4921 gccgtggtag aggcccgtgg tatcgccaag gcccaccgtg ttcgcggtgg agaacaggcg
     4981 gaacgacgga tgggggcgga tcacgcggtt ctggtcgagc agcgtcagct tgccctcgac
     5041 ttccagcacg cgctggatca cgaacatcac gtctgggcgg ccggcgtcgt attcatcgaa
     5101 taccagcgcg caggcgtgct gcagggccca cggcagaatc ccttcgcgga attcggtgat
     5161 ctgcttgccg tccttcagca cgatagcatc cttgccgatc agat
//
LOCUS       DATGEM010000172        11492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_178804, whole genome shotgun sequence.
ACCESSION   DATGEM010000172 DATGEM010000000
VERSION     DATGEM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178804"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VKQ27_05395"
     CDS             <1..1094
                     /locus_tag="VKQ27_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLY88399.1"
                     /translation="LCALVGLGVTGLIVVITEYYTSTAYRPVRSIALSSTTGHGTNVI
                     QGLAVSMEATALPAIVICVGIVVTYMIAGLFGIAVAVTTMLSLAGMIVALDAYGPVTD
                     NAGGIAEMADLPADVRVTTDALDAVGNTTKAVTKGYAIGSAGLASLVLFAAYTQDLQH
                     YFPQLKVDFALQNPYVVIGLIIGGLLPYLFGAMGMTAVGRAAGAVVVEVRRQFREIPG
                     IMEGTAKPEYGKAVDMLTKAAIREMIVPSLLPVLAPIVLWIIIDIVAGREAAFASLGA
                     MLLGTIVTGLFVAISMTSGGGAWDNAKKYIEEGHHGGKGSEAHKAAVTGDTVGDPYKD
                     TAGPAVNPMIKITNIVALLLLAILAGWLH"
     gene            1561..3057
                     /locus_tag="VKQ27_05400"
     CDS             1561..3057
                     /locus_tag="VKQ27_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88400.1"
                     /translation="MPHSLVVRITGKAMTTTCIRNAACIVAWDAANRRHAYLLGGDVA
                     FTDETLVYVGPKFDGAADATIDGTDTMVMPGLVDLHSHPSTEPFYRGVREEHGVPSMY
                     MSGLYERSVAMQPDPVARRSGKQVAFCEMLLSGVTSVADLSSIDEGWIDIAAQSGLRV
                     FLAPSYASARWHLENGWALKYRWDEPAGRRGLDAALALIAEAAAHPSGRLSGIVSPAQ
                     IDTCTEDLLHDSFAAAEDRDVPLTVHCAQSVNEFHIMVDRHGKSPIQFARDLGILSPR
                     TVLGHAMFIDEHSWVRWHTHSDLAALVETGTNVAHCPSPFARYGHMLEDFGRYRRAGV
                     NLGIGTDVAPHNVIEEMRLAAILARVSARDINTTSVGALFHAATVGGAEALGRSDIGR
                     LAAGAKADLVLVDLGNVWMRPARDPLRSLVFTGADRAVRTVFVHGTKVMEDGHVLTMD
                     HEAALAGVAEGQARMLRDAPSRDWAGRSAEEISPLSLPVLNDRRNQVP"
     gene            3268..4272
                     /locus_tag="VKQ27_05405"
     CDS             3268..4272
                     /locus_tag="VKQ27_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88401.1"
                     /translation="MTQYLAQRLLQLVLVLFGVSMVVFIAMHLLPGDVAQLMLGDHAT
                     AQSLARLRAQLGIDQPVWVQYLRFLGDALRGDLGTSLSTNHPALDDVLTAFPVTLQLT
                     LASLAIASVVGVPLGVLTAVRHGSKFDATVMAVTLFGVSMPIFWLGLMLLILFAAVLG
                     WVPIGGLMPVGMEPPRITGMSVIDTALTGDPAMLGAALQHLVLPACTLATVPLALITR
                     ITRAEVMTAANFDHVRTAYAKGLTGQRVILRHVLRNAAIPIVTVIGLQLGLLLSGAVL
                     TETIFSLPGIGRLMVDSILSRDYPVVQAGALFIAALFVVVNLLVDLSYAVLDPRISRP
                     "
     gene            4269..5138
                     /locus_tag="VKQ27_05410"
     CDS             4269..5138
                     /locus_tag="VKQ27_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88402.1"
                     /translation="MSGTAEVAVVAAPERSLWRAFRRTSARIGLAIALLFLVLTVTAP
                     WIAPYDPFDQDLSSALSPPSSDHLFGADQYGRDVLSRVMYGTRTALVAIVVAGGLALT
                     VGGALGLIAGFVGGAADTVIMRIVDVLLAFPYLLLALIIVAALGPSLTNSIIAIGIIY
                     TPQYARLMRGQVLAIQNAEFVWAARSVGATRRRIMLRHVLPNSFSPVAVMATLQAGSV
                     VVETAGLSFLGLGAQPPSPDWGALLADGHSYFLSAWWIATFPGLAIFLVVLGFNLIGD
                     ALRDQLDPRRRSR"
     gene            complement(5230..6789)
                     /locus_tag="VKQ27_05415"
     CDS             complement(5230..6789)
                     /locus_tag="VKQ27_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88403.1"
                     /translation="MTTIRAAFAAALLLCAPVAAHAADKTLVISIAADPTGFDPEAVA
                     NNTSGFIMATVFDSLVRYKPGTVEVEPGLAKSWDISPDGLTYTFHLRTGITFHDGSKF
                     DAHEYIQTLDRQLKKDDPNSIYKTGPVEGYEDFTFGSVASYSAVDDNTVQFKLKEPSA
                     AFLNSLAMVWNGVVSYQAAAKYGKDFRNHPVGTGPFIFREWKARDQIVLDANPNYWKG
                     KPKLDHIVFKIYPDAQAALLALQHGETQIMGDVATQVIPALRADHNLTVLTQPGLTVN
                     GMAMPNDTPPFNDKRVRQALNYAVDKEAINKALYGGLAVTMTSPLPEAQWGFDKSLKG
                     YPYDLAKAKQLLAAAGVKPGLKVELLSYNTPRGYNPAGADLAVALQGYLRKVGIEADI
                     QKSDMGAFLATVRSGNYHGLFLTGWSGDNGDPDNFVGELWGSYGMPVNDTSHYKDAEV
                     DKLMQQAAKEVDHAKRIAMYGQVQKMILDGAPWVFVNSTLQVRAARKDVHGFQLNPTQ
                     MFFDMDQVSLQ"
     gene            6938..7462
                     /locus_tag="VKQ27_05420"
     CDS             6938..7462
                     /locus_tag="VKQ27_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88404.1"
                     /translation="MLTLQPAAVPRPSFWSRDAAYKITRDETEIGRIDLFASPRENAD
                     AHIHIQRRTFEAHILITGRSRWAHVPSRWVMRLEGQDLHSAVWESGKTWVTDAEEGSE
                     RLRLRRSTFGGSIAIEREHDQTQLGDIKWLRARLVPRPIPRRLELATSLGLPETLEVF
                     LLWIAVADDFRNPG"
     gene            7500..7628
                     /locus_tag="VKQ27_05425"
     CDS             7500..7628
                     /locus_tag="VKQ27_05425"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HLY88405.1"
                     /translation="MIARSDTGNFAPGNAGVPFYMDIICPVLAITVVTLARRPRPP"
     gene            complement(7636..9201)
                     /locus_tag="VKQ27_05430"
     CDS             complement(7636..9201)
                     /locus_tag="VKQ27_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HLY88406.1"
                     /translation="MPAPNVQIDTNSHQFTHKQILKVLSGILLCIFLAAIDQTVVIPA
                     VPAIASDLNGFGHLAWIVSAYLLTCTAMTPIYGKLSDIYGRRKLLLVALAIFAASSAL
                     CALAQSLPQLVAARALQGIGGAGLMAMAQAAIADVVSPRERGRYQGYMASTWGLASVA
                     GPIIGGWVTDAFSWRWIFWINLPVALIAMVLCDRALRLLHRQAREARIDWAGAAMLTT
                     AVSAWLMVLSWGGVEMPWTAPPILGLGAVGAMLIAVLAWQERRFSDPLLPPRLFANPV
                     FTRGVAIAFCGALALFGGSFLLPLFFQLVRGANAALSGALVVPFLASNCVGAWFSGQL
                     ARRHGKLKAILLSGLIACAVGFVLLAFIGAGTPRWVLVMFQLVLGFGLGMVMPASLVC
                     VQNAAEKRDIGAATGCLLFLRSMGGAFGSTLVGAVLAGGFTRNLAVFGIHSHIDLGEV
                     RQSGATLPGVPADMLPHVQQALAGAFHLAFLSCAVAMVIAVVVTLGMRDVPLRSTSAE
                     EPHTEPEPAALAH"
     gene            9323..10111
                     /locus_tag="VKQ27_05435"
     CDS             9323..10111
                     /locus_tag="VKQ27_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLY88407.1"
                     /translation="MAVKVGIGGWTFAPWRGPFYPDKLPQKRELEFASRAVTSIEING
                     TFYGTQKRDSFLRWYEETPEGFVFSLKGPRFTTNRRVLAEAGESVDRFLGSGVLELKE
                     KLGPINWQMAATKAFDAPDFEAFLKLLPPERDGRKLRHVVEVRHASFVCPEFVALLRQ
                     YGVAAVVADHASYPLLADVTAPFVYARLQAASEQEPAGYSADALATWEKRASAWAKGK
                     APDDLPLIAERPPAARSRDVFIYMINGFKPKAPAAAMALIERLR"
     gene            10111..10722
                     /locus_tag="VKQ27_05440"
     CDS             10111..10722
                     /locus_tag="VKQ27_05440"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLY88408.1"
                     /translation="MDRLLQGYQRFRRDAWPDRRRLFEQLADRGQHPRAMVITCADSR
                     VDPSMIFDTGPGELFVVRNVASLVPPYAPDTAHHGTSAALEFGVRVLNVPDLLVMGHA
                     LCGGIRALLEGPPPEVADFVASWIAIAEPAKQRTLAHADKSQWQLRCEQEAVQLTLDT
                     LLTFPWIKERVVSGKLRLHGAHFDIRSGELSLLGTNGEFAVVT"
     gene            complement(10859..>11492)
                     /locus_tag="VKQ27_05445"
     CDS             complement(10859..>11492)
                     /locus_tag="VKQ27_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLY88409.1"
                     /translation="HIAQVRDATGASVAAVARIEQTIGEVSSIAGSIAAAVEEQQAAT
                     AEIARNVSETAAAANQMTSRTAEVSTEADNTGKHAVEVRENTVRLNTAVSELHHSVIR
                     VVRTSTTEVDRRVSARYSTDLPCHLSVAGQSHAARVTDISEHGAAIRGAPSVSLGTRA
                     ALQIDAVGQALWCVVRSNDDDTLHVAFEPDAAALAKIKPIVERLGQRRAA"
BASE COUNT         1963 a         3849 c         3649 g         2031 t
ORIGIN      
        1 tcctgtgcgc gctggtcggc ctgggcgtga ccgggctcat tgtcgtcatc acggaatact
       61 acacgtccac ggcataccgc ccggtgcgca gcatcgcgct cagttccacc accggccacg
      121 gcaccaacgt gatccagggc ctcgccgtat ccatggaggc gacggcgctg cccgccatcg
      181 tcatctgcgt cggcatcgtg gtcacctaca tgatcgctgg cctgttcggc atcgccgtcg
      241 ccgtcaccac catgctgtcg ctcgccggca tgatcgtggc gcttgatgcc tacggcccgg
      301 tcaccgacaa cgccggcggc atcgccgaaa tggccgacct cccggcggat gtccgcgtca
      361 cgaccgacgc gctggacgcc gtcggcaaca ccactaaggc ggtcaccaag ggctacgcca
      421 tcggctccgc gggcttggcc tcgctggtgc tgttcgccgc ctacacacag gacctgcagc
      481 actacttccc gcaactgaag gtcgatttcg cgcttcagaa cccatacgtc gtgatcggcc
      541 tgatcatcgg cggcctgctg ccctacctgt tcggcgcgat gggcatgaca gcggtgggcc
      601 gtgcggctgg cgctgtcgtg gtcgaggtgc ggcggcagtt ccgcgaaatc cccggcatca
      661 tggagggcac agccaagccg gaatacggca aggcggtgga catgctcacc aaggctgcga
      721 tccgcgaaat gatcgtgccg tcgctgctgc cggtgctggc gccgatcgtg ctgtggatca
      781 tcatcgatat cgtcgccggc agggaggcag ccttcgcctc gctcggcgcc atgctgctcg
      841 gcaccatcgt gaccggcctg ttcgtcgcga tctcgatgac ctcaggtggc ggcgcgtggg
      901 acaacgccaa gaagtacatc gaggaaggcc atcacggcgg caaaggctcc gaggcccaca
      961 aggccgcggt caccggcgac accgtcggcg atccctacaa ggacaccgcc ggtcccgcgg
     1021 tgaacccgat gatcaagatc accaacatcg tggcgctgct gctactggcg atcctggccg
     1081 gttggctgca ctgaggcagt cgcttccggt cgtctgatac caaaagcccc ggcccacacc
     1141 ggggcttttt ggtcaaagcg ctcgccggtc cgacggcccc agccgccctt gcagcttgtt
     1201 ccgccattca accgcctcat cggtcggttc gaagagccgc gccgctttgg catgtatcaa
     1261 tctgtatcct gttcccggtt ccgcgcgagg cattcatacc tccgcgccag ccgctcgagc
     1321 gcatcgcgga cgtccgaggt tgatgcgtgt tcagccaggc ggtgcacctc gacggcacgc
     1381 gcccgcaatt cggcaggcgt caaatcggac aaccgaggtc ggcgtggctg ctgcatgaca
     1441 gctccctccg ggagacgatc gcttcctgac agaaggcatc accgttggct gatccaggtc
     1501 aacgaaattg acaaattgac ctgcatcagg gggtcgtgtg ccaggtggac ttcagtcgac
     1561 atgccgcaca gtcttgtcgt ccggatcacg gggaaagcca tgacgaccac ctgcattcgc
     1621 aacgctgcct gcattgttgc ctgggacgca gcgaaccgcc ggcatgccta tctgctgggc
     1681 ggcgatgtcg cattcaccga tgagacgctg gtttatgtcg gtccgaaatt tgacggcgcg
     1741 gcggacgcga cgatcgacgg caccgacacg atggtgatgc ctggcctggt cgacctgcat
     1801 tcgcatccca gcaccgaacc attctatcgc ggcgtccgcg aggagcacgg tgtgccctcg
     1861 atgtatatga gtggtctgta tgaacgctcg gtggcgatgc agccggatcc ggtggcgcgc
     1921 aggtccggaa agcaggtggc attctgcgag atgctgctct cgggcgtgac cagcgtggcc
     1981 gatctctcca gcatcgatga aggatggatc gacatcgcgg cgcagagcgg gctgcgggtc
     2041 ttcctggctc cgtcctacgc ttcggcgcgc tggcacctgg agaatggctg ggcgctgaaa
     2101 taccgctggg atgaacccgc tggccgtaga ggattggatg cggcgctcgc gctgatcgct
     2161 gaggccgcgg cgcatccctc aggccgcctc tccggcatcg tctcgccggc gcagatcgac
     2221 acctgcaccg aggatctgct gcacgacagc ttcgccgccg ccgaagaccg cgacgtgccg
     2281 ctcacagtgc attgcgcgca gagtgttaac gagttccaca tcatggtgga tcgacacgga
     2341 aaatcgccga tccagttcgc gcgcgacctg gggatcctgt cgccgcgcac cgtgctgggt
     2401 catgcgatgt tcatcgacga gcattcctgg gtgcgttggc acacgcacag cgacctggcg
     2461 gcactcgtgg aaaccggaac caacgtcgcc cattgccctt caccgttcgc acgatacggc
     2521 cacatgctgg aggatttcgg ccgctaccgg cgcgccggcg tcaaccttgg catcggcacg
     2581 gacgtcgccc cacacaatgt gatcgaggaa atgcggctgg cggcgatcct cgcgcgcgtc
     2641 tcggcacgcg acatcaacac caccagcgtc ggtgctctgt tccatgcggc gaccgtgggc
     2701 ggcgccgagg ccctggggcg cagcgatatc ggccgcctgg ccgccggcgc caaggccgac
     2761 ctggtgctgg tcgatctggg caacgtctgg atgcgccccg cgcgcgaccc gctgcgttcg
     2821 ctcgtcttca ccggcgccga ccgcgccgtg cgcacagtgt tcgtgcacgg caccaaggtg
     2881 atggaggacg gccacgtgct gaccatggac cacgaagccg cattggccgg tgtcgccgag
     2941 ggccaggccc gtatgctgcg cgacgcaccg tcacgcgact gggccggccg cagcgccgag
     3001 gaaatctcgc cgctcagcct gccggtgctg aacgaccgac ggaaccaggt cccctaacac
     3061 ctctcccacc ccccatgcga gcggagaggc ctctcccggg gcttgacccg gggcgtcggg
     3121 tgaggtgggg gagggccttc gctcccaccc gcaggcgatc catttgagcg cggcacgcga
     3181 tcggatcacg aggcatcacc gcgatgctgg tgttgattgt ggcagagatc gtcggcacac
     3241 gtcgtgggtt atgagcgccc cctccacgtg acccagtatc tcgcccagcg cctgctgcag
     3301 cttgtgctcg tgctgttcgg tgtcagcatg gtggtgttca tcgccatgca cctgctgccg
     3361 ggcgacgtgg cccaactgat gttgggagac cacgcgacgg cccagtcgct ggcgcggctg
     3421 cgcgctcaat tgggcatcga tcagccggta tgggtgcagt acctgcgctt cctcggtgat
     3481 gcgctgcgcg gcgaccttgg cacgtcgctc tccaccaacc atccggcact ggacgatgtg
     3541 ctgaccgcat tcccggtgac actgcaactg acgctcgcct cactggcgat cgccagcgtt
     3601 gtcggcgtgc cgctcggcgt gctgaccgcg gtgcgccatg gctcgaagtt cgatgcgacg
     3661 gtcatggcgg tgacgctgtt cggcgtgtcg atgccaatct tctggctggg cctcatgctg
     3721 ctgatcctgt tcgcggcggt gctgggatgg gtgccgatag gcgggttgat gcccgttggg
     3781 atggagccgc ctcggatcac cggcatgagc gtgatcgaca ccgcgctgac cggcgatccg
     3841 gcgatgctgg gagcggcgct gcaacatctc gtgctgccgg cctgcactct ggcgaccgtg
     3901 cccctggcgc tgatcactcg catcacccgc gccgaagtga tgacggcggc gaactttgac
     3961 cacgtgcgca ctgcctatgc gaaaggcctc accggccaac gcgtgatcct gcgccacgtg
     4021 ctgcgcaacg cggccatccc gatcgtgacg gtgatcgggc tccaactcgg actgctgctc
     4081 tcaggcgcag tcctcacaga gacaatcttc tcgctgcccg gcatcgggcg cctgatggtc
     4141 gattcgatcc tgtcgcgcga ctatccggtg gtgcaggccg gcgccttgtt catcgccgcg
     4201 ctgttcgtcg tggtgaacct gctggtggac ctgagctacg ccgtgctcga tccacggatc
     4261 tcgcggccat gagcggcacc gccgaggtcg cggtcgttgc cgcgcccgag cgatcgctgt
     4321 ggcgggcatt ccggcgcacc agcgcccgca tcgggctcgc gatcgcgctg ctgttcctcg
     4381 tgctcacggt cacggcgccc tggatcgcgc cgtacgatcc cttcgatcag gatctatcga
     4441 gcgcgctgtc gccgccgtcc tcggatcatc tgttcggcgc agatcagtat gggcgcgacg
     4501 tgctgagccg ggtgatgtac ggcacgcgta ccgcgctggt ggcaatcgtt gtggctggcg
     4561 gattggcgct aaccgtgggc ggcgcgctgg gcctgatcgc cggcttcgtc ggcggagcag
     4621 ccgacaccgt gatcatgcgc atcgttgatg tactgctggc gttcccctat ctgctattgg
     4681 ctctgatcat cgtcgcggca ctcggcccaa gcctgacgaa ctccatcatc gccatcggca
     4741 tcatctatac accgcaatat gcgcgcctga tgcgcggcca ggtgcttgcg atccagaatg
     4801 ccgagttcgt ctgggcagca cgttcggtag gcgccacccg acgccgcatc atgctgcggc
     4861 acgtgctccc taacagtttc tctcccgtcg ccgtcatggc gacgctccag gcaggctccg
     4921 tggtggtcga aaccgccggc ctgtcgttcc tcggcctcgg ggcgcagccg ccgtcaccgg
     4981 attggggcgc gctgctcgcg gacggccata gctacttcct cagcgcctgg tggatcgcca
     5041 cctttcccgg cctggccatc ttcttggtcg tactgggctt caacctgatc ggcgacgcac
     5101 tgcgcgacca gttggaccca cggcggagaa gcagatgatc ccccgccccc gtcattgcga
     5161 ggaggcgtag ccgacgaagc aatctcgctc ggattgctcc gtccgcaacc tcccgaagac
     5221 aggtggctgc tactgcagcg acacctgatc catgtcgaag aacatctgcg tcggattcag
     5281 ctggaacccg tgcacatcct tgcgcgccgc ccggacctgc agcgtcgagt tgacgaacac
     5341 ccagggcgcc ccatccagga tcatcttctg aacctgccca tacatcgcga tccgcttcgc
     5401 atggtccacc tccttggcgg cctgctgcat cagcttatcc acctcggcgt ccttgtaatg
     5461 cgaggtatcg ttcaccggca tcccatagga accccaaagc tcgcccacga aattatccgg
     5521 atcgccgttg tcgccgctcc acccggtaag gaacagccca tggtaattcc ccgaccgtac
     5581 agtcgccaga aacgccccca tatcgctctt ctgaatatcg gcctcgatgc caaccttgcg
     5641 tagatacccc tgtaatgcca ccgccagatc ggcccccgca ggattgtacc cacgcggagt
     5701 gttgtagctc agcagctcca ccttcaaccc cggcttcaca ccggccgccg ccagcaactg
     5761 cttcgccttc gccagatcgt acgggtaccc cttcagcgac ttgtcgaagc cccactgcgc
     5821 ttccggcagc ggcgacgtca tggtgaccgc aagtccgcca tacagcgcct tgttgatcgc
     5881 ctccttgtcc accgcgtagt tcagcgcctg ccgcacccgc ttgtcgttga acggcggcgt
     5941 gtcatttggc atcgccatgc cgttcaccgt cagcccaggc tgggtcagca cggtaaggtt
     6001 gtgatcagcg cgcagcgccg ggatcacctg cgtcgcgaca tcgcccatga tctgcgtctc
     6061 gccgtgctgc aacgcgagca gtgcagcctg cgcatctggg taaatcttga acacgatgtg
     6121 gtcgagcttc ggcttgccct tccagtagtt cgggttggcg tccagcacga tctggtcgcg
     6181 cgccttccac tcacggaaaa tgaatggccc ggtgccgacg gggtggttgc ggaaatcctt
     6241 cccgtacttc gccgctgcct ggtagctgac cacgccattc cacaccatcg ccaggctgtt
     6301 gaggaatgct gccgacggct ccttcaactt gaactgcact gtgttgtcgt cgaccgcgct
     6361 gtacgacgcg acggaaccga aggtaaaatc ctcatacccc tcgaccgggc cggtcttgta
     6421 gatactgttc ggatcatcct tcttgagctg ccggtccagc gtctggatgt attcgtgagc
     6481 gtcgaacttg ctgccgtcat ggaaggtgat gccggtccgc aagtggaacg tataggtcag
     6541 gccgtccgga gaaatgtccc agctcttcgc aaggcccggc tcaacctcca ccgtgcccgg
     6601 cttgtagcgc accaggctgt cgaacactgt cgccatgatg aagcccgagg tgttgttggc
     6661 gaccgcctcg ggatcaaacc ccgtgggatc cgcggcgatg ctgatcacca gcgtcttgtc
     6721 cgccgcgtga gcggcgaccg gggcacacag aaggagagcg gcggcgaacg cagcgcggat
     6781 ggtcgtcatg gcgaaggcct cactggcggc tgaaggtcca gcttagcatc gggctgcatg
     6841 ccggagccaa ggggcgcccc tcatgcagtg cggcggaggg cgcgaagcgc tgccagttga
     6901 tgttacagtc atgatccgtt tgaagaggcg cgagacaatg ctgacgcttc agcccgccgc
     6961 tgtgccgcgg ccctcgttct ggtctcgaga cgcagcctac aagattacgc gcgacgagac
     7021 tgagatcggg cggattgacc tgtttgccag ccccagggag aacgcggacg cgcatatcca
     7081 cattcagcgg cgaaccttcg aggcccacat tctgattact gggcgatcac ggtgggcgca
     7141 tgtgccatcg cgttgggtca tgcggcttga gggccaggat ttgcacagcg cggtgtggga
     7201 gagcggcaag acgtgggtca ccgacgcgga ggaggggagc gagcgtcttc gacttcgcag
     7261 atcaacgttt ggcggctcga tcgcgatcga gcgcgaacac gaccagactc aactcggcga
     7321 tatcaaatgg ctcagggccc gcttggtgcc aaggccaatc cctcgccgct tggaactcgc
     7381 gacaagtctc gggctgccgg agacgcttga ggtgtttctc ctgtggatcg ctgtcgcgga
     7441 cgacttcaga aacccgggct gattgcagcg aagtgtctca tcagcaacga gggcaacgga
     7501 tgatcgcgcg gtccgatact ggcaatttcg cgccaggcaa cgccggtgtt ccgttctaca
     7561 tggacatcat ctgtcctgtg ctcgccatca cggtcgtgac gctcgcaagg cgcccgcgtc
     7621 cgccctgagc cccagtcagt gcgccagcgc agctggctcc ggctcggtgt gcggctcctc
     7681 ggcggaggtg gatcgcagcg gcacgtcgcg catccccagc gtcacaacga cggcgatcac
     7741 catcgccacg gcgcacgaca ggaaggccag atgaaaggcg ccggccagcg cctgctgtac
     7801 gtgaggcaac atgtcggccg gcacacctgg aagcgttgca ccgctctggc ggacctcgcc
     7861 gaggtcgata tgcgagtgga tgccgaacac cgccaggtta cgcgtgaagc cgcccgccag
     7921 caccgcgccc accaacgtgc tgccgaaggc gccgcccatc gagcggagga acagcaggca
     7981 gccggtggcg gcaccgatgt cgcgcttctc ggctgcgttc tgcacgcaga ccaggctggc
     8041 gggcatcacc atcccaaggc cgaagccaag tacgagctgg aacatcacca gcacccagcg
     8101 cggcgtcccc gcgccgatga acgccagcag cacaaacccg acggcgcacg cgatgagccc
     8161 gctcagcaga atggccttca gcttgccatg ccggcgcgcg agctggccgg agaaccaggc
     8221 accgacgcag ttcgaggcaa ggaacggaac caccagggcg ccggagagcg cggcattcgc
     8281 tccccggaca agctggaaaa acagcggcag cagaaaactg ccgccgaaca gcgccagcgc
     8341 gccgcagaag gcgatggcga cgccccgcgt gaacaccgga ttggcaaaca ggcgtggcgg
     8401 cagcagcggg tcagagaacc ggcgctcctg ccaggcgagc accgcgatca gcatcgcgcc
     8461 gacggcgccg agaccgagaa tagggggcgc cgtccacggc atctcgacgc cgccccagct
     8521 cagcaccatc agccaggcac tgaccgcggt agtcagcatg gcagctcccg cccagtcgat
     8581 gcgggcctcg cgggcctggc gatgcagcag ccgcagcgca cggtcgcaca gcaccatggc
     8641 aatcagcgcg accggcaggt tgatccagaa gatccagcgc caggagaacg cgtcggtgac
     8701 ccagccgccg atgatcggtc ccgcgaccga cgccaggccc caggtagatg ccatatagcc
     8761 ctgatagcgg ccacgctcgc gggggctcac cacgtcggcg atggcggcct gggccatcgc
     8821 catcaggccg gccccaccga taccctgcaa cgcccgggcg gccaccagct gcgggaggct
     8881 ctgcgccagg gcgcacaggg cggatgacgc ggcgaagatg gccagcgcga ccagcaggag
     8941 cttccgacgg ccgtagatat cggaaagctt gccgtatatc ggtgtcatcg ccgtgcaggt
     9001 cagcaggtag gccgagacga tccacgcgag gtggccgaag ccgttcaggt ctgatgcaat
     9061 tgcggggacg gccgggatga cgacagtctg gtcgatcgcg gcgaggaaaa tgcagagcag
     9121 gatgccgctt aagaccttca ggatctgctt gtgggtgaac tgatgcgagt tggtgtcgat
     9181 ctgcacgttc ggcgctggca tgaacccgtc ctgctgtgtc ggcgacctgg ctcacgatca
     9241 cgacaggtct gctggccttt atactgcgct gcaacatgag cagccacggc gtatggttca
     9301 actctgatct gcgggaggat gcatggcggt aaaggtggga atcggcgggt ggacgttcgc
     9361 gccctggcgt gggccgttct atccggacaa gttgccgcag aagcgtgaac tcgagttcgc
     9421 gagccgggcg gtgacctcga tcgagatcaa cggcacattc tacggaacgc agaagcggga
     9481 cagcttcctc cgctggtacg aggaaacgcc agaggggttc gtgttctccc tgaagggccc
     9541 ccggttcacc acaaaccgtc gcgtgcttgc cgaggcaggc gaatcagtgg accggttcct
     9601 cggcagcggc gtgttggagc tgaaggagaa gctcggaccg atcaactggc agatggcggc
     9661 gaccaaggct ttcgacgcgc cagacttcga ggcgttcctg aaactgctcc cgccggaacg
     9721 cgacggccgg aagctgcgcc acgtcgtgga ggtgcgccac gccagcttcg tgtgccccga
     9781 gttcgtcgcc ctgctgcggc aatatggcgt cgccgcagtg gtagcagatc atgcgagcta
     9841 tccgctgctc gccgatgtga cagcgccgtt cgtgtatgca cggttgcagg cggcatccga
     9901 gcaggaaccc gcaggctact ccgccgatgc cctggcaacc tgggaaaagc gcgcaagcgc
     9961 ctgggccaag ggaaaggcgc ccgacgacct gccgctgatc gccgaacggc caccggctgc
    10021 gcggtcaagg gacgtgttca tctacatgat caacggcttc aagccgaagg ccccggctgc
    10081 cgccatggcc ttgatcgagc ggctccgctg atggaccgct tgcttcaggg ctatcagcgc
    10141 tttcgccgcg acgcctggcc cgaccggcgc cgcctgtttg agcagctcgc cgaccgcggc
    10201 cagcacccgc gtgcaatggt gatcacctgc gccgacagcc gcgtcgatcc gagcatgatc
    10261 ttcgacaccg gcccaggcga gctgtttgtc gtccgcaacg tcgccagcct cgtcccgcct
    10321 tatgcccccg acactgccca tcacggcacc agcgccgccc tggagttcgg tgtgcgcgtg
    10381 cttaacgtcc ccgacctgct ggtgatgggt cacgccctct gcggcggcat acgcgcgctg
    10441 ctcgaaggcc cgccgcccga agtcgcggac ttcgtcgcca gttggatcgc catcgccgaa
    10501 ccggcgaagc agcgaaccct ggcgcatgcc gacaagtcac aatggcaact tcgctgcgag
    10561 caggaggctg tgcagctgac gctcgacacc ctcctgacgt tcccctggat caaggagcgc
    10621 gtggtgtccg gcaagctgcg gctgcacggt gcgcatttcg atatccgctc gggagaacta
    10681 agcctgctgg gcacgaatgg tgagttcgcc gtcgtcacgt gatctctcgt gcgtcgtcat
    10741 ggccgcccgc cgcccccttc gtcatggccg gccttgagcc ggccatcgtt cgtcgcaggc
    10801 acaacgtcgc gttgcgagcc cgatgtccag ctacgacctg tcggtgccag tcacgcagct
    10861 atgccgcgcg ccgctggcca aggcgttcca cgattggctt gatcttggcc agagccgccg
    10921 catccggctc gaatgccacg tgcagtgtgt catcgtcatt gctgcgcacg acgcaccaca
    10981 gtgcctggcc caccgcatcg atttgcagcg cggcacgcgt gcccaacgaa accgacggcg
    11041 ccccgcgaat ggccgcgccg tgctcggata tgtccgtcac tcgtgccgcg tgactctgac
    11101 ccgcaacgct cagatggcat ggcagatcgg tactgtagcg ggcgctgacg cggcgatcga
    11161 cctcggtcgt agaggtgcgg acaacccgga tcaccgaatg gtgcaattcg ctgacggcgg
    11221 tattcagccg aaccgtattc tcccgcactt caactgcgtg cttgccggta ttgtccgctt
    11281 cagtggatac ctcggcagtg cggctcgtca tctggttcgc cgccgccgcc gtctcgctca
    11341 cattgcgggc gatctcagcg gtcgctgcct gctgctcttc caccgcggcg gcaatagagc
    11401 cggcgattga actgacctca ccgatagtct gctcgatgcg cgcaactgcg gccaccgaag
    11461 cgcctgtggc atccctgacc tgagcgatat gc
//
LOCUS       DATGEM010000173         4336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1205927, whole genome shotgun sequence.
ACCESSION   DATGEM010000173 DATGEM010000000
VERSION     DATGEM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205927"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKQ27_05450"
     CDS             complement(<1..644)
                     /locus_tag="VKQ27_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLY88410.1"
                     /translation="MAQHQLRASPQTVRIGVFDASYPPVMTIESGDRVVVQCVSGRQE
                     VMPPASAGLTVPPELDAIIAANPGMRAGHIVTGPIAIAGAEPGDMLEIRIEKIEPGAD
                     WGYNTIRPLAGTLPEDFHETVISHIPVDKARMVCSLPWGTELALAPFFGVMGVAPPPA
                     FGTIASKEPRIHGGNMDNKELVAGSTLYLPVWVSGANFSVGDGHGVQGDGEVCVT"
     gene            complement(656..2062)
                     /locus_tag="VKQ27_05455"
     CDS             complement(656..2062)
                     /locus_tag="VKQ27_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY88411.1"
                     /translation="MSDILTIAEAARLIAAKKLSPVELTKACIERTRALDAELHAFIL
                     PTEDRALADARTAEAAIMAGKSPGPLCGIPIGLKDIVDTKGIPTTCHSKILKDNVPTS
                     DAMCAVKLAEAGSVMMGKTATHEFADGGPSFDLPWPPARNPWNTGHFTAGSSSGTGAG
                     VAAGLIMGGIGTDTGGSIRGPAALCGIAGIKPTYGLVSRAGVAPAAFTLDHIGPMAWT
                     AEDCAIMLQALAGHDPADPASASQPIPNYTEKLGAGIKGMRIGVIHHFHESDHQVTPA
                     VQHGIDAAIATFRELGAEIREVTLSPMPEWHACGTLISITERASAYDEWARTRLADFG
                     ERVQQRLLVGALVSGVDYVQAVRRRRELRAELKAAMADLDVVLTAGNPSEAPKIDDVP
                     MWDVSAKPSFTMPFNVTGYPAMCVCSGFGDSDLPVSIQLVGKPFQEATVLRIADAFEK
                     ATPFRNRRPAMSSALVPA"
     gene            complement(2059..2256)
                     /locus_tag="VKQ27_05460"
     CDS             complement(2059..2256)
                     /locus_tag="VKQ27_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88412.1"
                     /translation="MPPELDDATLDFLLARSGLSPTPRQKAELKAIYPYVAAMAERVR
                     KPRGRMAEPVLTYGFTKEDLA"
     gene            2370..3566
                     /locus_tag="VKQ27_05465"
     CDS             2370..3566
                     /locus_tag="VKQ27_05465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY88413.1"
                     /translation="MTSENETLSGPLAGIRVIDLTTVVLGPFATQILGDMGADVIKIE
                     SPDGDNCRWIGPRRNKGMGAYFAMLNRNKRSVMLDLKQPTAREALLRMIDTADVFAHN
                     MRLGAADRLGLSYAALSARNPRLVYACASGFRKGSSKQEHPAFDDLIQGVSGLAALNA
                     GPDGAPRYVPSVVVDKLTGQTLASMIGMALFHRERTGKGQEVHVPMLETMLSFLLVEH
                     MWGMALNQPELGMGYPRMLTPHRRPYATKDGYISVIAVSDNHWRRLFEAMGRGGLIED
                     QRFATIAARSDNVDALYAVLTEGMQERTTAEWLEILGALDIPCGPANTLTDLLDDPYL
                     QETGFFQHMAHPRDGDVVITAIPARFSASPPSVRRLWPALGEHTEEVLREIGLDAGGI
                     RAVVGG"
     gene            complement(3649..>4336)
                     /locus_tag="VKQ27_05470"
     CDS             complement(3649..>4336)
                     /locus_tag="VKQ27_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY88414.1"
                     /translation="SDEVQPILRQIAPQARLFVRWDNVPDYAAFCALSGLPRLAGTRV
                     DTIPAPIPYLRADPARISRWADRLSGLVPGGYRRVGVIWAGRPTHNNDRNRSVLLADF
                     MPLANVAGIALLALQKGPRTGQAGEYYGRAPLINIGAEIDDYDDTMAILENLDLLVTV
                     DTSVAHLAGAMGRQVWVMLPRAPDWRWLLGTSETSWYPTMRLFRQQNARQWQDVVKAI
                     ADELAGNARS"
BASE COUNT          740 a         1372 c         1413 g          811 t
ORIGIN      
        1 gtgacgcaga cctcgccatc gccctgcaca ccgtgtccgt cacccaccga gaagttggcg
       61 cccgataccc acactggcag atacagggtg gaacccgcca ccagctcttt gttgtccatg
      121 ttgccgccgt ggatgcgcgg ctccttggaa gcgatggtgc cgaatgccgg cggtggtgcg
      181 acgcccatga cgccgaagaa gggcgccagg gccagctcgg tcccccacgg cagcgagcag
      241 accatccgtg ccttatcgac tgggatgtgg ctgatcactg tctcgtggaa atcttccggc
      301 agcgtgccgg cgagcgggcg gatcgtgttg tagccccagt cggcacctgg ctcgatcttc
      361 tcgatgcgga tctccagcat gtcgccgggt tccgctccgg cgatggcgat cgggcccgtg
      421 acgatatgcc ccgctcgcat gccgggattg gccgcgatga tcgcatcgag ttccggcggc
      481 acggtcagcc cggcgctggc cggcggcatc acctcttgcc ggccagagac gcactgcacc
      541 acaactctgt cgccggactc gatcgtcatc accggtggat agctcgcgtc gaatacgccg
      601 atgcgaacgg tctgcggcga ggcgcgaagc tgatgctggg ccattgtgct ttccttcagg
      661 cgggaacgag ggcggatgac atggcggggc ggcgattgcg aaacggtgtt gccttctcga
      721 acgcatcggc gatgcgcagc accgtcgctt cctggaacgg tttgccgacg agctggatgg
      781 agaccggcag atcgctgtcg ccaaagcccg agcacacgca catcgccgga tagccggtca
      841 cgttgaacgg catggtgaag ctcggcttcg ccgacacgtc ccacatcggc acgtcatcga
      901 tcttgggcgc ttcggaggga ttgcccgcgg tcagcaccac gtcgagatcc gccattgccg
      961 ccttcagttc ggcgcgcagt tcgcgacgac ggcggaccgc ctggacgtaa tcgacgcccg
     1021 atacgagtgc gcccaccagc aagcgctgct gcacacgttc accaaagtca gccaggcgcg
     1081 tgcgagccca ttcgtcatat gcggacgcgc gttcggtgat cgagatcagc gtgccgcacg
     1141 cgtgccattc cggcatcggc gacagagtca cttcgcggat ttcggcgccg agctcgcgga
     1201 acgtggcaat cgctgcatcg atgccgtgct gcacggcagg cgtgacctgg tgatcgcttt
     1261 cgtggaagtg gtggatcacg ccgatgcgca tgcccttgat gccggcacca agcttttcgg
     1321 tgtaattcgg aatcggttgc gaggcgctgg ccggatcagc cggatcgtgg cccgcgagcg
     1381 cctgcaacat gatcgcgcag tcttcggcgg tccatgccat cggtccgata tggtcgaggg
     1441 tgaaggcggc cggtgcgacg cccgcgcggc ttaccagccc gtaggtcggc ttgattcccg
     1501 cgatgccaca gagtgccgct ggcccgcgga tcgagccgcc ggtatccgtg ccgatgccgc
     1561 ccatgatcag ccctgccgcg acgccggcgc cggtgccgct cgacgaaccg gcggtgaagt
     1621 gcccggtgtt ccagggattg cgcgcaggcg gccacggcag atcgaatgag ggcccgccat
     1681 cggcgaactc gtgcgtcgcg gtcttgccca tcatgacgct gccggcctcg gccagcttca
     1741 ccgcgcacat cgcatcgctg gtgggcacgt tgtccttcag gatcttcgaa tgacacgtcg
     1801 ttgggatgcc tttggtatcg acgatgtcct tcaggccgat cgggattccg cagagcgggc
     1861 ctggggattt gcccgccatg atcgctgctt cggcggtgcg tgcatctgcg agggcccggt
     1921 cttccgtcgg caggatgaag gcgtgcagct cggcatccag cgcgcgagtg cgctcgatgc
     1981 aggccttcgt cagctcgacc ggggacagct tcttcgcggc gatcagcctg gcggcttcag
     2041 cgatggtgag gatgtcgctc atgccaggtc ctccttggtg aagccatagg tgaggaccgg
     2101 ttccgccatg cgaccacgtg gcttgcgcac gcgctctgcc atcgcggcga cgtaaggata
     2161 gattgccttc agctctgctt tctgtcttgg cgtgggcgaa agtccggagc gcgccaacag
     2221 aaaatcgagt gtggcgtcat ccagttctgg cggcatgctc ccctccggcc gatatgttgc
     2281 gacgcagcat agtggccgca tcagcttgcg tgcaagtgga cgggcattgt gacatgagag
     2341 cctgacgatg gttgatgatg agcgatttga tgacaagtga aaacgaaacg ctgagcggtc
     2401 ctcttgcggg gatccgggtg atcgacctga caacggtggt gttgggtccg ttcgccacgc
     2461 agatccttgg cgacatgggt gccgatgtca tcaagataga gtcgcctgat ggcgacaact
     2521 gccgctggat cggccccagg cgcaacaaag gcatgggcgc ctacttcgcc atgctgaacc
     2581 gcaacaagcg cagcgtgatg ctggatctga agcagcctac agcgcgcgag gcgctgctgc
     2641 gcatgatcga taccgccgac gtgtttgcgc acaacatgcg gctgggcgca gcggatcgtt
     2701 tggggctgag ctacgccgcg ctttccgcgc gaaatccgag gttggtctat gcgtgcgcgt
     2761 ccggctttcg gaagggcagc agcaagcagg aacatcccgc gttcgacgac ctgatccagg
     2821 gcgtgagcgg gcttgcggcg ctgaacgctg ggccggatgg cgccccgcgt tatgtcccga
     2881 gcgtggttgt cgacaagctc accggccaga cgctcgcatc gatgatcggc atggcgctgt
     2941 tccatcgcga gcgcacaggc aaaggccaag aggtgcacgt gccgatgctg gagacgatgc
     3001 tgtcctttct gctggtcgag cacatgtggg ggatggcgct gaaccagccg gagctcggca
     3061 tggggtatcc tcgcatgctc acaccacatc gccggcctta cgcgacgaag gacggataca
     3121 tttccgtgat tgcggtcagc gataaccact ggcgccggtt gttcgaagcg atggggcgcg
     3181 gtgggctgat cgaggatcag cgcttcgcca ccatcgctgc ccgttccgac aacgtggacg
     3241 cgctttacgc cgtgctgacc gaaggcatgc aggaacgcac cactgcggag tggctggaaa
     3301 ttctgggcgc gctcgacatc ccttgcggtc ccgccaacac gctgaccgac ctgctggacg
     3361 atccgtatct acaggagacc ggcttcttcc agcacatggc gcatccgcgc gatggggatg
     3421 tggtgataac cgcgattccg gctcggtttt ccgccagccc accatctgtc cgtcggctct
     3481 ggcccgcgct tggcgaacat acggaagagg tactgcgcga gatcgggctg gacgcaggcg
     3541 gcattcgagc ggtcgtcggc ggctagacac tgctcgagat tgcttcgtcg ctccgctcct
     3601 cgcaatgaca ggaggatcgg acatcgcgcc ttccttggtc atcggtgctt atgaccgggc
     3661 gtttcctgcc aattcgtcgg cgattgcctt caccacgtcc tgccattgcc gggcattctg
     3721 ttgccggaac agccgcatcg tcgggtacca cgatgtttca gacgttccca atagccagcg
     3781 ccaatcgggc gcacgaggca gcatgaccca tacctgccgc cccatcgccc ccgccaggtg
     3841 agcgacggac gtatctacag tgaccaacag atcgaggttc tcgaggatgg ccatcgtgtc
     3901 atcgtaatcg tcgatctccg cgccgatatt gatcagcggc gcgcggccat aatattcgcc
     3961 ggcctggccg gtccttggcc ccttctgcaa tgccagcagc gcgattcccg ccacgttcgc
     4021 cagtggcatg aaatcggcca gcagcactga gcgattgcga tcgttgttat gcgtcggccg
     4081 ccctgcccag atcacaccga cacgccgata tccgcccggc accagtccgc tgagccgatc
     4141 ggcccagcgc gagatgcgcg cggggtcggc gcgcaggtat gggattggcg ccgggattgt
     4201 atccacccgc gtcccggcga gccggggcag ccctgacagt gcacagaacg ccgcatagtc
     4261 aggcacattg tcccatcgca cgaacagcct tgcctgtggt gcgatctgcc gcaggatagg
     4321 ctgcacctcg tcactg
//
LOCUS       DATGEM010000174         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1370545, whole genome shotgun sequence.
ACCESSION   DATGEM010000174 DATGEM010000000
VERSION     DATGEM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370545"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VKQ27_05475"
     CDS             <1..642
                     /locus_tag="VKQ27_05475"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="HLY88415.1"
                     /translation="AATSDMAPDAPPISEDWMNVFESEAAHMGSEQMQHLFGKILAGE
                     IRRPTSYSIKTIRLVAQLDNQAASLFKRLCSLSISLRLPSFGTIHDARVVSMGDANAN
                     SLQAYGLGFDALNILHEYGLIISDYNSHMDYRISAAYGGNIILPMTYQGAKWGLFPKV
                     NPQDPKEFKVNGVVFSRSGKELLSIVEVDEDKQYTSALHAFLDTQGMQMVMLP"
     gene            complement(639..1334)
                     /locus_tag="VKQ27_05480"
     CDS             complement(639..1334)
                     /locus_tag="VKQ27_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLY88416.1"
                     /translation="MANGRFVAYYRVSTEKQGASGLGLEAQREAVMRHLNGGQWELIG
                     TFTEVESGKRNDRPELAAALELCQATGATLVIAKLDRLARNVAFVSNLMETGVEFVAA
                     DFPQANRLTVHILAAVAEHEREMISQRTKMALKAAATRRAESGLSKLGNPANLTEAAA
                     AKGRDMGRAARIAKADARAASVARIIARIQAEGHISHAAIARELTARCIETPRGGSNW
                     QVIQVQRILDRTT"
     gene            complement(1420..1896)
                     /locus_tag="VKQ27_05485"
     CDS             complement(1420..1896)
                     /locus_tag="VKQ27_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88417.1"
                     /translation="MALSLNGADELRANLQRVAAAALAAGRAELGRIAHDVEQGSIER
                     APYKEGDLERAHEVQAVTRPAGIRFVVKVDESVAPYAIYMHEGFVAKDGTPEMPYKLG
                     VASMMKNAGTPHMGAGVGWKFLERSFTDATADAEIRLAGAISTAIGQSVGVTVRRR"
     gene            complement(1900..2784)
                     /locus_tag="VKQ27_05490"
     CDS             complement(1900..2784)
                     /locus_tag="VKQ27_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88418.1"
                     /translation="MPSSIRRDTSLPGVAALTLEDFEAHGRGEITLGALADRLGVSRQ
                     AVRAAFKRRGWTSSGVSSADEAPPPLPGATVKVEAAPLAVESEPADAVELKERIGAEL
                     VQADVLLLGIIGRQLRHWHDTGTALGASQMKAAAAALSGIRENLIQAGLVTPAEDAER
                     PIELRVRSLSVEEEAEIRRAAEADDGEQSDGEEGQEAERAGGDDDAPPAPSALLAAPL
                     SPPVSARFIIDPVPVEAANFPVWLQRLALTRGARTLRQIIDALGGDPPAAGSGADLVT
                     TILNLTAGDPARLSHLRK"
     gene            complement(2762..2998)
                     /locus_tag="VKQ27_05495"
     CDS             complement(2762..2998)
                     /locus_tag="VKQ27_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88419.1"
                     /translation="MNSERTMADMKQRLNDLQRIIDDPIAVLSDEQVDEYVGLRATYL
                     EAHRKALEAREAEELRLASLRGKTRIANALQHPA"
     gene            complement(3003..3239)
                     /locus_tag="VKQ27_05500"
     CDS             complement(3003..3239)
                     /locus_tag="VKQ27_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88420.1"
                     /translation="MTLELLAELMGVSIDSARKLVAVMPRKSTEDEALAAIKALWLTE
                     SGGPVAEWLAAHGGAVCHIAERLIAIREPSAAEG"
     gene            complement(3323..4015)
                     /locus_tag="VKQ27_05505"
     CDS             complement(3323..4015)
                     /locus_tag="VKQ27_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88421.1"
                     /translation="MHAPGLGNMSCHPDVGAPLVAGVSLQPLSPAQADRFRSLGAEWV
                     IPANRAEAALLEVATAPAGLSGVYLAALDTLLKQVRDYPAEAARLLPPIAGDEASVIA
                     GMIKNLDDALPTPPAGLYEGADFLRQHLNASHGLATTAIAVALGKLDSTRAKIAAAES
                     ELSAIRVELGRQMEQATAPESIEDARRRRVSAKAARLMPALIADMERLRDEAAGIVGQ
                     LDKTLLALRGVA"
BASE COUNT          852 a         1475 c         1430 g          916 t
ORIGIN      
        1 gccgccacga gcgacatggc gccggacgca cctccgatta gcgaagactg gatgaacgta
       61 ttcgagagtg aggcggccca tatgggctcc gaacagatgc agcatttgtt cggaaagatc
      121 ctcgcgggcg aaattcgaag acctacgtct tactcgatca agacaattag gctcgtggcg
      181 cagttggaca accaagccgc ctccttattc aagcggctct gctcgttatc catctcgttg
      241 cgcctgccga gcttcggcac tattcacgat gcccgtgtag tctcaatggg ggacgcgaac
      301 gcgaactcgc tgcaggctta tggcctagga ttcgatgccc tcaatatact gcacgagtat
      361 gggctgatta tctctgacta caattcgcac atggactatc ggatatcagc cgcctacggc
      421 ggcaatatca tattgcccat gacctatcaa ggagctaagt ggggattgtt cccaaaggta
      481 aatccacagg accccaaaga atttaaagta aatggcgtag tgttttctcg atcaggcaag
      541 gagcttctct ctatcgtaga ggtcgacgaa gacaaacaat acaccagtgc tttacacgct
      601 tttctcgata cacagggcat gcagatggta atgctccctt aagtcgtgcg atccaggatc
      661 cgctgcacct gaatcacttg ccaattgcta ccaccgcgcg gtgtctcgat gcagcgggca
      721 gtaagttcgc gagcgatcgc ggcgtggctt atgtgacctt cggcctggat cctggcgatg
      781 atcctagcga cgctagccgc cctagcgtca gccttcgcga tgcgagcagc ccgccccatg
      841 tcgcgcccct tcgccgccgc cgcttccgtc aggtttgccg gattgccgag cttcgacagc
      901 ccgctctcgg cgcggcgtgt tgctgcggcc tttaacgcca ttttggtgcg ctgcgagatc
      961 atctcgcgct cgtgctcagc aacggcggca aggatatgca ccgtcaggcg gttagcctgg
     1021 gggaagtcgg cggcgacgaa ctcgacgccg gtctccatca agttcgatac gaaggcgaca
     1081 ttccgggcga ggcgatcgag cttcgcgata acgagggtcg cgccggtcgc ttgacatagt
     1141 tcaagcgcgg cggcgagttc cggccgatca ttgcgcttcc cgctctcgac ctcggtaaag
     1201 gtgccgatca gttcccactg gccgccatta aggtgccgca tgaccgcttc gcgttgcgcc
     1261 tccaggccaa ggccgctcgc accctgcttt tcggtggaga cccggtagta tgcgacgaag
     1321 cggccgtttg ccattttcga aggtccttac aaatcagtgc gcgttcgttg actgaattgt
     1381 aagtagctcg gccgcctgcc gtcaacgcct tttgtgaacc tatcgccgcc ggaccgtgac
     1441 cccgacggat tggccgatcg ccgtggagat ggcgccggcg agccggatct cggcatctgc
     1501 ggtggcgtcc gtgaagctcc gctctagaaa cttccagccg acgcccgcgc ccatatgcgg
     1561 cgtgccggca ttcttcatca tcgaggcgac gccgagtttg taaggcatct ccggcgtgcc
     1621 atccttggca acgaagccct cgtgcatgta gatcgcgtag ggcgcgaccg actcatcgac
     1681 cttgaccacg aagcggatgc ccgctggcct cgtcactgct tggacttcgt gggctcgctc
     1741 cagatcgcct tccttgtaag gcgcccgctc gatggatcct tgttcgacgt catgggcgat
     1801 ccgcccgagt tcggccctgc cggcagccaa tgcggcggcc gcgaccctct gtaggttcgc
     1861 gcgcagttca tctgcgccat tgagtgatag agccatctgc tacttcctaa gatgcgacag
     1921 ccgagcagga tcgccggccg tcaggttgag aattgtggtc acgagatcgg cgccgctccc
     1981 ggctgccggt gggtcgccgc ccaatgcgtc gatgatctga cgcagcgtcc gcgcgccgcg
     2041 cgtcagggcg aggcgctgca gccacaccgg aaagttcgcg gcctcgaccg gcacgggatc
     2101 aataatgaag cgggcggaca ccggcggcga caacggtgcg gccaagaggg cagacggtgc
     2161 tggcggcgcg tcgtcatcgc caccggctct ctcggcctcc tggccttctt ccccatcgga
     2221 ctgttcgccg tcgtcggcct cagcggctcg ccggatctca gcctcctctt ccaccgaaag
     2281 cgatcggacg cgaagctcga tcggccgttc ggcgtcttcg gcgggagtta cgaggccggc
     2341 ctggatgaga ttctcacgaa tgcccgacaa cgcggcggcg gcggctttca tctgcgacgc
     2401 cccgagtgcc gtcccggtgt cgtgccaatg gcggagttgt cggccgatga tcccgaggag
     2461 cagaacgtcc gcctgcacga gttcggcgcc gatccgctct ttcagttcga cagcgtcggc
     2521 cggttcggac tcgacggcca ggggtgcagc ttctaccttg accgttgcgc cgggcagcgg
     2581 cggcggggct tcgtcggcgg aggacacccc cgacgaagtc cagccgcgac gcttgaacgc
     2641 ggcgcggacg gcctgccgtg agacgccgag ccggtcggcg agcgctccta gggtgatctc
     2701 gccgcgaccg tgcgcctcga agtcctcaag cgtgagcgca gcgacgcccg gtaagctggt
     2761 gtcacgccgg atgctggagg gcatttgcaa tccgagtctt gccgcgtagc gatgcgagtc
     2821 gaagttcctc ggcttcgcgc gcctctagcg ccttcctgtg agcctctaga taggtcgcgc
     2881 gcaacccgac atattcgtcg acctgctcat cggagaggac ggcgatcggg tcatcgatga
     2941 tccgctgaag gtcgttcagc cgttgcttca tatccgccat cgtacgttcg ctattcatgg
     3001 cgtcacccct ccgccgcgct gggctcgcgg atggcgataa gccgctcggc gatgtggcag
     3061 acggcgccgc cgtgcgccgc caaccactcg gccaccggcc cgccgctctc ggtcagccag
     3121 agcgccttga tggctgccag cgcctcatcc tcggtgctct ttcggggcat cacagcgacc
     3181 agcttgcgcg cggagtcaat cgagaccccc ataagttcgg ccagcaattc gagcgtcatc
     3241 ggtccgtgct gccgacccca cacccaggca agccggatcg ctaagcgctt cgcggccgag
     3301 aatttgtgat ttcgcgcggc catcaggcga cgccccgcag cgccagcagc gtcttgtcga
     3361 gctgcccgac gattccggca gcttcgtcgc gcagacgctc catgtccgcg atcagcgcgg
     3421 gcatcagccg ggcggccttg gccgacaccc gccggcgccg tgcgtcttcg atgctctccg
     3481 gcgcggtcgc ctgctccatc tggcgcccga gttcgacgcg gatcgcagac agttcgctct
     3541 cggcagccgc aatctttgct ctggtgctgt cgagcttacc cagagcgacg gctattgcgg
     3601 tggtggcgag accatggctg gcgttcagat gctgacgaag gaagtccgct ccctcgtaga
     3661 gcccggccgg tggcgtcgga agtgcgtcgt caaggttctt gatcatgccg gctatgacgc
     3721 tggcttcatc gcccgcaatc ggcggcaaca gccgcgcagc ctcggcggga tagtcgcgca
     3781 cctgcttaag caaggtgtcg agggcggcca gatagacgcc ggacaggccg gccggcgcgg
     3841 tcgcgacttc cagtagggcc gcctcggcac gattcgccgg aatcacccac tctgcgccta
     3901 acgagcgaaa gcggtcggct tgagccggcg agagcggttg aagggagaca cccgcgacta
     3961 gtggggctcc cacgtcggga tgacaagaca tgttgccaag gcccggtgca tgcaagaagt
     4021 tcatcgtttc gactctggat gatttacgat ttctttataa tacaaaattt gaccatcaca
     4081 gtcaaaaatg gcggaatcca tgagttctca agagaatgtc ttagaattaa cggaacgtac
     4141 gttgacataa ttgccaatct ctagcgatat ctagcagcat ctattgatat gtaaatccgg
     4201 aacattttcg attgcgcgtg atgtggcggc ttgctatgac ggcgctagga gaccgctagg
     4261 atttgatatg cgcaacgagc aagtgaacgt gagagtccag gccgagccgc ttgatgatcg
     4321 gcccctaatc cggcaggtcg cggcgaagct gcgcaatgat ccggacttcc gcgcgcgcct
     4381 aggggcgctc ctaggcgaca atgcgtcggc atcgagtgat cttgttgacc gcgtgtcgaa
     4441 actcgaacag gcggtctcgg accttcgcag ggctcgtcaa cccaacgttt tcaccgacgc
     4501 agcctcagca gccccaaccg atgccgggaa gtcagcatga agtcgatcgc cgcaatttac
     4561 cgccgagtct caaccgtctc gcaagcgggc gaggaccggg tatccctcag cgaccaggaa
     4621 aatggttgcc gaaggatggc gacggctgat ggcttcaccg ttcccgacca cat
//
LOCUS       DATGEM010000175         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_65820, whole genome shotgun sequence.
ACCESSION   DATGEM010000175 DATGEM010000000
VERSION     DATGEM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65820"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2643)
                     /locus_tag="VKQ27_05510"
     CDS             complement(4..2643)
                     /locus_tag="VKQ27_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88422.1"
                     /translation="MPDELRRPEFDPAILLDAEAEVVARTARGEIAVFPEAERPVLRA
                     AFLRHLLLQLSPNDWPIRLPGVRIHAARIEDTLDLADSVGLPGLALVACELAAPLDLT
                     RARIARLSLRDSRIGEVRARGTRVEGVMDISGVAPLGETAWIDAHAAQIEGDLIARAA
                     RLQIPPPREGIAHRDARYALRLSGAEIRGSLDLMGGFVAIGGISLDTAHVTGDVVARG
                     AGVGAGEGDAVGAQAARFDGVVIFGDGFVAAGVIWLMGARIAGTLDMNGARLVNRTED
                     GQGVVLAADTAEIGGSVLLRNGFTAEGAVSVRGARIGNSLECDGANLDNATPDGAGIA
                     LAADHVTVGGAVLLRNGFQARGAVSLIGAKLGGNLECDGSTLANATPDGTGVALAAEN
                     ASIGGAVLLRHGFVARGSVSLLGATIGSNVECCGAVLENWPDTGSRETLRLTNAEIAG
                     DVLLNQGFASLGYVSLWGTNIGRDFDCSSATLIGPSPVEASRSMAGAMVATNLSVAGD
                     MKLIGTTVLGRLDCVSLQTGGSLIWDGLRFPRQVVRDDWRHGYQTGVDALPRIRLAHA
                     RIGAALMARDLTAETPMTVDLSGARAVTLDDEGFPAGWGVGRDADGLFCHLNLDGFVY
                     ERFGHLPSDEGSGIGITLSALGRWAITVGKGHTATRALYLARRLVRAQRTHVRQRLAW
                     VQRQQRRASEFHPQPYRHLAKVLRTQGHYEAAREVAIAEQWAKPAANAVTRLLRPVWG
                     VCFGFGFSPVRATATLLVLLAIGTGGVWWAWKGAHVLSINYSYAMTEVANGPVFAEPP
                     KDQGTTGAPECGGNDIQPVFYAMDMMLPVIALQEASRCWVDTRPGTEVWQVLWAIFSF
                     VGKLATSLALLTYSGVLKPKEEA"
     gene            complement(2701..3471)
                     /locus_tag="VKQ27_05515"
     CDS             complement(2701..3471)
                     /locus_tag="VKQ27_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY88423.1"
                     /translation="MPELDTRRQELKEAFVAARGYWSELWDGVLELSPEFFEAYSALS
                     SVPWKYGTLPPKLKELIYVAVDASTTHLYNPGTRIHIANALKHGATRDEVMEVLEIVS
                     VLGIHTMSTGLPILIDELRNAGRGEEVREGALSPEQQKLKQDFVTNRGYWNPVWEQLL
                     QHSPAFFDAYTRLSSVPWKHGTLEPKFRELIYIAIDAATTHLYLPGLRTHVRNALGHG
                     ATVAEIMEVLQLTSTIGIHTVTEGVPILLEEARHAGKR"
     gene            complement(3488..>4860)
                     /locus_tag="VKQ27_05520"
     CDS             complement(3488..>4860)
                     /locus_tag="VKQ27_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88424.1"
                     /translation="DQLFGLDENFGVQPQMAAGYELSDDKLRWRITLREGLSFHDGDK
                     VRAADCVASINRWSKRDPFGVRMAGLLDEMKSIDDNSFEIRLKRPYAQMLYGLGATTC
                     FIRPERLAKTDIGTQITDATGSGPFRFVANEFVTGDHAAYARFDKYVPRQEKASGWAG
                     GKQANFERVEWKFIPDPATAAAALQTGEVDWVERPLFDLIPKLKSTSGVSVDLVDPFG
                     YFGILYLNNAAPPFDNAKLAHAILPALNQADCMQAVLGDLQGLSHPNMGMFTPGSPFA
                     SDAGMDILTGKRDVALSKRMIAASGYKGEKVVIMVPEIPEARAMSEVTRALFQELGLN
                     VDYQSMDWGTLSARARSTDPAVRAGWSCYCVGWAGLWPAVPGSNIPIGGVKPNPKMEA
                     LRTAWFDAPDLPAQKKLAADMQILGLEEPPFLPLGQYFFPYGFRSDLSGFVKAGITAM
                     WNVKRS"
BASE COUNT          898 a         1701 c         1462 g          799 t
ORIGIN      
        1 cacctatgcc tcctccttcg gcttcagcac accggaatag gtcagcagcg ccagtgatgt
       61 cgccagcttt ccgacgaacg agaagatcgc ccacagcacc tgccacacct cggtccccgg
      121 ccgcgtatcg acccagcacc gcgacgcctc ctgcaacgcg atgaccggca gcatcatgtc
      181 catcgcgtag aacaccggct ggatgtcgtt gcccccgcat tccggcgcac ccgtggtacc
      241 ctggtccttc ggcggttcgg cgaacaccgg accgttggcg acctcggtca tcgcgtagga
      301 gtagttgatc gacagcacgt gcgctccctt ccaggcccac cacacgccgc ccgtgccgat
      361 ggcgagcagc accagcaagg tggccgtcgc acgcaccggc gagaaaccga agccgaagca
      421 cacgccccag accggacgca gcagccgcgt cacggcattc gccgccggct tggcccattg
      481 ctcggcaatc gccacctcgc gcgccgcctc gtaatgtccc tgcgtgcgca gcaccttcgc
      541 cagatggcga tagggctgcg ggtggaactc actggcgcgc cgctgctgac gctgcaccca
      601 ggcaagccgc tgccgcacat gcgtccgttg cgcacgcacc agccggcgcg ccagataaag
      661 tgccctggtc gccgtgtggc ccttaccaac cgtgatagcc caccggccga gcgccgacag
      721 tgtgatgccg ataccgctcc cctcgtcgga tggcagatgc ccgaaacgct cgtagacaaa
      781 tccatccaga ttcaaatgac agaacagacc gtcagcgtcg cggccgacgc cccagccggc
      841 ggggaaacct tcatcgtcca gtgtcacggc ccgcgcgcca ctgagatcga cggtcatcgg
      901 cgtctccgcc gtcaggtcgc gcgccatcag cgccgcaccg atgcgggcat gcgccagccg
      961 gatgcgtggc agagcatcga caccggtctg gtagccgtgc cgccagtcgt cgcggaccac
     1021 ttgccgcgga aaacgcagtc cgtcccagat caggctgccg ccagtctgca ggctcacgca
     1081 gtcgagccgg cccagcacgg ttgttccgat cagcttcata tcgccggcga ccgaaagatt
     1141 ggtggcgacc atcgcacccg ccatcgagcg gctcgcctct accggcgagg gcccgatcag
     1201 cgtcgcactc gagcaatcga agtcgcggcc gatattggtg ccccacaagc tgacatagcc
     1261 gaggctggcg aagccctggt tcagcagcac atcgccggcg atttccgcat tggtcagccg
     1321 cagcgtctcg cgcgagccgg tatcaggcca gttctctaag acagcgccgc agcattcgac
     1381 gttgctgccg atggtagcgc cgagcaggct gacactgccg cgcgcgacga acccatgccg
     1441 cagcaggaca gcgccgccga tcgaggcgtt ctctgcggcg agcgctacgc cggtcccgtc
     1501 cggcgtagca ttggcgagcg tcgagccgtc gcattccagg ttgcccccaa gcttggcgcc
     1561 gatcaggctc acagcacctc gcgcctgaaa cccgttccgc agcaacaccg ccccgccgac
     1621 ggtgacgtga tccgctgcga gcgcaatgcc ggcgccatcc ggcgtggcat tgtccaggtt
     1681 ggcaccgtcg cattccaggc tgttgccgat acgcgcgccg cgcacgctga ccgcgccttc
     1741 agcggtgaag ccgttgcgca gcaggacgga tccgccgatc tccgccgtgt cggcggcaag
     1801 gacgacgccc tgaccatcct cggtgcggtt caccagccgg gcgccgttca tatccagcgt
     1861 gccggcgatg cgcgcgccca tcagccagat aacgccggcg gcgacgaaac catcgccgaa
     1921 gatgaccacg ccatcgaagc gggcggcctg ggcaccgacg gcatcgccct ccccagcccc
     1981 gacgccggcg ccacgcgcca caacgtcccc ggtgacatgc gcggtgtcca aggaaatgcc
     2041 gcctatggcg acaaagccgc ccataagatc caggctgccg cggatttcgg cgccggaaag
     2101 acgcagcgca tagcgggcgt cgcgatgggc gatgccctcg cgcggcggcg ggatctgcaa
     2161 cctggccgca cgagcgatca gatcgccctc gatctgcgcg gcatgcgcgt cgatccaggc
     2221 agtctcgccc agcggtgcaa caccagagat gtccatcaca ccttcgacgc gcgtcccgcg
     2281 ggccctcacc tccccgatgc ggctgtcgcg aagcgacagg cgggcaatgc gcgcacgtgt
     2341 caggtcgagc ggcgcagcca actcgcaggc cacgagcgcc aggccaggca gaccaacact
     2401 gtcggcaaga tccagcgtgt cctcgatgcg cgcggcgtgg atacgcacgc caggcagacg
     2461 gatcggccaa tcgttgggag acaactgcaa gagcagatgg cgcaggaagg cagcgcgcag
     2521 gacagggcgt tctgcctccg gaaaaaccgc gatctccccc ctagcggtcc gggcgaccac
     2581 ctcggcttcg gcgtcgagca ggatcgccgg atcgaactcc ggtctgcgca gttcgtcagg
     2641 catcgctctg ttcgggcagc acggccgaac agtaacgaag cgtgctcagt cggcaaagca
     2701 tcagcgttta ccggcgtgtc gcgcctcctc cagcaggatc ggcacgccct ccgtgacggt
     2761 gtggataccg atagtgctgg tgagctgcag cacctccata atctccgcta ccgtcgcccc
     2821 atgacccagc gcgttgcgga catgcgtccg gagcccgggc agatacagat gcgtcgtcgc
     2881 ggcgtcgatc gcgatgtaga tcagttcgcg gaacttcggc tcgagcgtgc catgcttcca
     2941 ggggacagac gacaggcgtg tatacgcgtc gaagaacgcc ggtgagtgtt gcaggagctg
     3001 ttcccagact gggttccagt agccgcggtt ggtgacgaag tcctgcttca gcttctgctg
     3061 ttcgggggag agcgcgcctt ccctcacctc ctcgccgcgg ccggcattgc gcagctcgtc
     3121 gatcagtatc ggcaggcccg tggacatcgt atggatgccg agcaccgaga cgatctccag
     3181 cacctccatc acctcatcgc gcgtcgcgcc atgcttcagc gcattggcga tgtggatacg
     3241 cgtgccggga ttatagagat gcgtcgtgga ggcatcgacc gccacgtaga tcagctcttt
     3301 cagcttcggc ggcagtgtgc cgtatttcca gggaactgac gacagcgcgc tataggcttc
     3361 gaagaactcc ggcgagagtt ccagcacgcc gtcccacagt tcgctccaat agccgcgcgc
     3421 agcgacgaac gcctctttca gctcttgccg acgagtatcg agttccggca ttggctcttc
     3481 ctctccctta gcttcgcttg acgttccaca tcgcggtgat ccccgccttc acgaagcccg
     3541 acaaatcgga gcggaaccca tacgggaaaa aatactgacc cagcggcagg aacggcggct
     3601 cctccagtcc aagtatctgc atatccgccg ccagtttctt ctgggcagga agatcaggtg
     3661 catcgaacca tgccgtgcgc agcgcctcca tctttgggtt cggcttcacg ccgccgatcg
     3721 ggatgttgct gccaggcacc gccggccaca gccctgccca gccaacgcaa taacagctcc
     3781 agccggcgcg cactgccgga tcagtgctgc gcgcccgtgc cgatagcgtg ccccagtcca
     3841 tcgactggta gtcaacattc agccccagct cctggaacag cgcccgcgtg acttctgaca
     3901 tcgcccgtgc ctcggggatc tctggcacca tgattaccac tttctcgcct ttgtagcccg
     3961 acgccgcgat catcctcttc gacagcgcga catcgcgctt gccagtcagg atgtccatgc
     4021 cggcatccga cgcgaacggc gatccaggcg tgaacattcc catgttgggg tgcgacagtc
     4081 cctgcagatc gcccaatacc gcctgcatgc agtcggcctg gttcagcgcc ggcaggatcg
     4141 catgcgccag tttcgcattg tcgaacggag gagccgcgtt gttgagatac aggatgccga
     4201 aatagccgaa cggatcgacc agatcgaccg acacaccgga cgtcgatttg agctttggga
     4261 tgagatcgaa gagcggccgt tccacccagt ccacttcgcc ggtctgcaag gctgccgcag
     4321 ccgttgccgg atcaggaatg aacttccact ccacgcgctc gaaattcgcc tgcttgccgc
     4381 ccgcccaacc cgatgccttc tcctgacggg gcacgtattt gtcaaagcgc gcatacgcag
     4441 cgtgatcgcc ggtgacaaac tcattggcga caaagcggaa cggaccgctg cccgtcgcgt
     4501 cggtgatctg cgtgccgatg tcggtcttgg ccagccgctc tggccggatg aagcaggtgg
     4561 tcgcgccgag gccatacagc atctgcgcat acggccgctt caggcggatc tcgaagctgt
     4621 tgtcgtcgat ggacttcatt tcatcgagca ggccagccat ccgcacgccg aacggatcac
     4681 gcttcgacca gcggttgatc gaggcaacac aatccgccgc gcgcaccttg tcgccgtcat
     4741 ggaacgacag gccttcacgc agcgtgatgc gccagcgcaa cttatcatcc gaaagctcat
     4801 agccggcagc catctgcggc tgcacgccga agttctcgtc cagaccgaag agctggtcgc
//
LOCUS       DATGEM010000176        12581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1318683, whole genome shotgun sequence.
ACCESSION   DATGEM010000176 DATGEM010000000
VERSION     DATGEM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318683"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VKQ27_05525"
     CDS             <1..400
                     /locus_tag="VKQ27_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HLY88425.1"
                     /translation="QRQEANGAHWLEASHDGWKKLFGAVHRRRLYVAESGEDVRGEDV
                     VEAPSPQPFTLRFHLHPDVNASLQQDGEAVLLRLRSGSGWRLRADGARMSLEESVYLG
                     GHEPRRSEQVVLTGYADGPQQVKWAISKVG"
     gene            376..1554
                     /locus_tag="VKQ27_05530"
     CDS             376..1554
                     /locus_tag="VKQ27_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY88426.1"
                     /translation="MGDQQGGIELSSPTLSGPRAGEGKKLKIIEVTNVDFSLVQFLLP
                     LMRALRARGHDVIGVCAEGPLLEAPRAEGFRIVAPRLERTASPLAHWRAFRDMVRLFR
                     EERPDLVHAHMPISGFLARMAAWWVGVPRIAYTGHGFWFNFPGSWPRAVVGFAMEWLA
                     ARVTGTFLTVSEAEMRDARRLHIHARAMAVRNGRDPMRFHPDSEARRQVRAELGVADG
                     QVVVLAVSRLVWHKGYPELAAALGEVPDAVLWVAGERLVSDRGPDMEALLREAGLGER
                     LRLLGYREDVPALMAAADIFVLPSRFEGLPMSVIEAMLTVLPVVACDVRGPAEQVVEG
                     VTGLLVPVGDAPALAGALRRLTEDTELRARMGAAGRARAIERYDEAKVLARTIELLEL
                     "
     gene            1618..3192
                     /gene="purH"
                     /locus_tag="VKQ27_05535"
     CDS             1618..3192
                     /gene="purH"
                     /locus_tag="VKQ27_05535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLY88427.1"
                     /translation="MPDTIPIRTALISVSDKSGLVPLAQALVAQGARIISTGGSARAL
                     RDAGVPVTEVSEHSGFPEILDGRVKTLVPQIHGGILGRRDLPEHVAQMVQHGIAPIDM
                     VAVNLYPFEGTVARGARYDECVENIDIGGPALIRAAAKNHDFVTVLTEPEQYAEVLEE
                     IARSGGTSLALRRRLAGAAYARTAAYDAAIAAWFAEQRGDTFPARLTLAGTLRQTLRY
                     GENPHQSAAFYVSGDRLGIATAAQVGGKELSYNNLNDTDAAFECVAEFEAPTVAIIKH
                     ANPCGVASAGTLAEAWDLALRCDPVSAFGGIVAVNRVLDTAAAEKIASIMTEVIIAPD
                     ADPGAKAALARRRNLRLLLTGGVPDAAAGGLTFRSVAGGFLAQTRDNGRVGRGDLKVV
                     TQRAPTEAELADLLFAFRVCKHVKSNAIVYAKDAATVGIGAGQPSRVDSARIGAWKSE
                     EAAKAAGLDVLLARGSVAASDAFFPFADGLEAAIAVGATAVIQPGGSVRDKEVIAAAD
                     AAGIAMVLTGMRHFRH"
     gene            complement(3281..4519)
                     /locus_tag="VKQ27_05540"
     CDS             complement(3281..4519)
                     /locus_tag="VKQ27_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HLY88428.1"
                     /translation="MLRRLALAAAFAVASLGAAHAAEPITIGFGMALTGGLAPNGKAA
                     LLAMQIWESDINAKGGLLGRPVKLVYYDDQSNPATIPGLYTKLLDVDKVDLVVSGYAT
                     NMIAPAMPIIMQHNRTFLSLLGLAANTEFHYPKYFSFTPTGGPEPKQSFAAGFFATAM
                     AQNPKPQTLAIVGADAEFPHNAMDGARAIAKKEGLKVVYDKTYPPTTTDYTPIVRAIQ
                     ATNPDLVLVCSYPPDTVGMIRAAHEVGLKAKLFGGGMVGLQSTAIKAQLGPLLNGIVD
                     YDFWLPWSKSASPEAKAFLEQYQAKAGAAGVDPLGYYLPPFAYGYMQVLQQAVEGTKS
                     LDQDKLADYLRSHTFKTVVGDVKFGPDGEWSEARVLEVQFQNVKANDVNQFKDANTEV
                     ILWPTDLKTGTLMYPYSDVK"
     gene            4639..5454
                     /locus_tag="VKQ27_05545"
     CDS             4639..5454
                     /locus_tag="VKQ27_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY88429.1"
                     /translation="MRMVFDPEAVRAFERSRWQQAARAYETSFATATTPFIDALLDSA
                     GVAAGTRMLDIACGPGVVAAQAVARGAVARGLDFSPAMLAIAIDRGIAADEGDAEALP
                     YMDASFDAVVSNFGVHHVPRPALALSEAHRVLSARGQVAFSFWAEHAENIAWKLVFDA
                     VARHGDPATSDAPPPGGGFRGAAQCAEALRAAGFVDCTTQLVHATWRHADAAELLDAL
                     QTGTARMAAMIDAQPRAAIPAIIADIEDNAARYRTSDGIAIPIAAVIAAGTKS"
     gene            5472..5816
                     /locus_tag="VKQ27_05550"
     CDS             5472..5816
                     /locus_tag="VKQ27_05550"
                     /inference="COORDINATES: protein motif:HMM:NF042718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6614 family protein"
                     /protein_id="HLY88430.1"
                     /translation="MYHAWCDLKPGISDTGFADKVAAYMGHLKEQGLIEGWRLTRRKL
                     GLGGPGLGEFHLMIEVKDLAQLEQAFERVAGRREPVEGFHFGVNSLVQNAVFALYRDF
                     PDTFRHRGEERF"
     gene            5838..6332
                     /locus_tag="VKQ27_05555"
     CDS             5838..6332
                     /locus_tag="VKQ27_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88431.1"
                     /translation="MLLLRVLSLAGTALLVGAAAPDLPSVTGQSPVAAGEYLVAIGGC
                     NDCHTEGWMMNPGKVPAAQRLTGSHVGWNGPWGTSYPTNLRLLVQKMSADAWVQHAHT
                     MQPRPPMPWYNMRSMSEPDLKAMYVYIRSLGPAGEPAPDALPPGQKPATSYIRAEPVP
                     PQKG"
     gene            complement(6329..7408)
                     /gene="rseP"
                     /locus_tag="VKQ27_05560"
     CDS             complement(6329..7408)
                     /gene="rseP"
                     /locus_tag="VKQ27_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLY88432.1"
                     /translation="MLDLLRSAAAFIVVLGVLVFVHEFGHYIAARWRGVHVEVFSIGF
                     GPAIATWRDRVGTVWKLAWLPLGGYVKLHGQERAEDVSDDVRATWMPGRLFHEKRVGS
                     RAIVVAAGPIANFLLAIVLFAALFATAGKPTILPVAGGVLPDSAAAHAGLQVNDRIIA
                     IDGKPTSSFGDIQTIVSAHPSEKLTLTVRRGDTTLTLPVVTDSHMSGDTKVGLLGISG
                     GQIEYQRLSLPAALAGGVTQTWDITRETIVGVWQMITGKRGTEDLGGPLRIAQLSGAV
                     AEQGPLYLIQFIALLSVNLGLLNLFPIPVLDGGHLLFYLAEALRGRPIPPRVQEYGFR
                     AGLALLLGLFILATWNDLTRPGVFH"
     gene            complement(7442..8611)
                     /locus_tag="VKQ27_05565"
     CDS             complement(7442..8611)
                     /locus_tag="VKQ27_05565"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLY88433.1"
                     /translation="MTSAPRSVTVLGSTGSVGTQTVELLIAEPQRFQVRALVAGRNAT
                     LLAQQAIALKANRAVIADASCYMELRAALDGTGIDTAAGAEAVVEAAALAADWTMAAI
                     TGAAGLEATLAAIRRGAVVALANKEALVCAGDVMLQAVKEAGATLLPVDSEHNAIFQV
                     LEDENRHAVEKIVLTASGGPFRTATRDEMAAATPDMARKHPVWSMGAKISIDSATLMN
                     KGLELIEAARLFGLQEPQIDVLVHPQSVIHGMVCYHDGSVLAQLGSPDMRIPIAHTLA
                     WPERMSTPSPRLDLATLARLDFMPPDTERFPALRLAREALREGGGAPAILSAANEIAV
                     EAFMQRRIGFLDIAGTVAEVLDAMGTHSADTLDEVVAVDAAARRTAERLTSARAA"
     gene            complement(8608..9363)
                     /locus_tag="VKQ27_05570"
     CDS             complement(8608..9363)
                     /locus_tag="VKQ27_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLY88434.1"
                     /translation="MSGDSVPDLAKHQPGRWSDLRIRILSAAVLTPIGVASVWLGGVA
                     FILIVGIIATGLVIEWLLLFRPQAPRIEPRPAWACLPPTRPGLFSAGLLYVAIAGTSL
                     LWLRADPVSGRADVLFVLLIVWAGDIGAYVVGRCIGGPRLAPHISPGKTWSGAGGGLI
                     AAVATGLLTAHFLATPVTPWQPALIAVVLSTVAQAGDLLESFVKRLLAVKDSGNLIPG
                     HGGLFDRLDGVLAAAPVAALLALALGRGVVLWQ"
     gene            complement(9332..10006)
                     /gene="uppS"
                     /locus_tag="VKQ27_05575"
     CDS             complement(9332..10006)
                     /gene="uppS"
                     /locus_tag="VKQ27_05575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HLY88435.1"
                     /translation="MDGNGRWAASRHLPKIAGHREGARAVRRTIQAAIENGVAWLTIY
                     AFSSENWRRPAGEVLDLTGLLRQYLKSEIAELKANGVRLHFIGDRAKFDPDIGADLTN
                     AERDTASNTRLNLIVALSYGARAEIVEAARALADAARSGRLEPGQIDESMLAGSLATA
                     GIPDPDLIIRTSGEQRLSNFLLWQAAYSELVFLDVLWPDFGAEHFAAALAEYARRERR
                     FGARPG"
     gene            complement(10040..10609)
                     /gene="frr"
                     /locus_tag="VKQ27_05580"
     CDS             complement(10040..10609)
                     /gene="frr"
                     /locus_tag="VKQ27_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLY88436.1"
                     /translation="MPQADVDTLKQDLTRRMDGALDTLKREFNGLRTGRAHPGLLEPV
                     KVQAYGTEMPLTQCGTVGAPEPRMLTVQVWDRSLVGAVEKAIRDAGLGLNPAADGQLV
                     RVPIPQLTTERRTELVKAAHKYAEGAKVAVRGVRRDGMEQIKTHEKKHDIGEDVAKDW
                     SAEVQKLTDQYIKRVDDSLADKEKDIKQV"
     gene            complement(10640..11365)
                     /gene="pyrH"
                     /locus_tag="VKQ27_05585"
     CDS             complement(10640..11365)
                     /gene="pyrH"
                     /locus_tag="VKQ27_05585"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLY88437.1"
                     /translation="MPDQPVYQRVLLKLSGEALMGRREYGLDNETVRAIATDVRDVVA
                     MGVQVCIVIGGGNIFRGVSGAASGMERAQADYMGMLATVMNALAMQSALEKIGVPTRV
                     QSAIPMSSVCEPYIRRRAERHMEKGRVVIFAAGTGNPYFTTDTAAALRANEMQCQALF
                     KGTQVDGVYSADPRKVSGATRYEQLTYLDVLANDLAVMDAAAISLARENRLPIVVFNI
                     HAPGAFAAVIRGEGRFTKIIELQ"
     gene            11535..12227
                     /locus_tag="VKQ27_05590"
     CDS             11535..12227
                     /locus_tag="VKQ27_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88438.1"
                     /translation="MTARPASGRLLASFLTDLIASVVIVLMLGWLVQASAGNDYLRDA
                     IVAQNDIYVSISRFTPHNLGMSYVSTVVQMNRGVGFGYQPGEGYTDVIQYGLVTVAIV
                     ILNVFRALPDTILELYHETSGTAAWIVLGGFAVGVGSVSVALLSMRSSPWRLVLAILA
                     SPFAISLIFLALQGFMIAMMDTFYWFTSLAPYTVACPVICTLYWVAFPNAERGATATL
                     AQAMLRVLDPKR"
     gene            complement(12255..>12581)
                     /locus_tag="VKQ27_05595"
     CDS             complement(12255..>12581)
                     /locus_tag="VKQ27_05595"
                     /inference="COORDINATES: protein motif:HMM:NF042722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6635 family protein"
                     /protein_id="HLY88439.1"
                     /translation="AISSFPLGAGLGSLWYGMYPASAAPLLAVGLSGGLLMIGAVLSA
                     FAGIVTDPVQRRIGLHQRRLRRMIDAMERQMLDPAAPGYSVLDRYVARLIDVLDVVTA
                     IVRLMR"
BASE COUNT         2102 a         3755 c         4450 g         2274 t
ORIGIN      
        1 gcagcggcag gaggcgaacg gcgcgcattg gctggaagcg agccacgatg gttggaagaa
       61 gctgttcggc gcggtgcatc ggcggcggct gtatgttgcg gaaagcggcg aggacgtgcg
      121 tggcgaggat gtggtcgagg caccgagccc gcagccgttt acgctgcggt tccatctgca
      181 tcccgatgtg aacgccagct tgcagcagga tggcgaggcg gtgctgctgc ggctgcgctc
      241 gggcagcggc tggcggctgc gggcggatgg ggcgcggatg agcctggagg agagcgtcta
      301 tctcggcggc catgagcctc ggcgcagcga gcaggtggtg ttgacgggat atgctgatgg
      361 gccgcagcag gtgaaatggg cgatcagcaa ggtgggatag agctgagttc ccccacctta
      421 tccggtccca gggccggaga ggggaagaag cttaagatca ttgaggttac caacgtcgac
      481 ttttccctgg tccagttcct gctgccgtta atgcgcgcgc tgcgggcgcg cgggcatgat
      541 gtgatcgggg tgtgcgccga gggaccgctg ttggaggcgc cgcgcgcgga agggttccgg
      601 atcgtggcgc ctcggttgga gcggacggcg tcgccgttgg cgcactggcg ggcgttccgc
      661 gacatggtgc ggctgttccg tgaggagcgg cctgacctgg tgcatgcgca catgccgatc
      721 agcgggttcc tggcgcggat ggcggcgtgg tgggtcgggg tgccgcggat tgcctacaca
      781 gggcacgggt tctggttcaa ttttcccggg tcgtggccgc gcgcggtggt tggcttcgcc
      841 atggagtggc tggcggcgcg ggtaaccggc acgttcctga ctgtcagcga ggcagagatg
      901 cgcgacgcac gacgattgca tatacatgcg cgtgcgatgg cggtgcggaa tgggcgtgat
      961 ccgatgcgct tccacccgga ttccgaagcg cggcggcagg tgcgcgcaga gcttggggtg
     1021 gctgacgggc aggtggtggt gctcgcggtg tcgcggctgg tttggcacaa gggatatccg
     1081 gagctggcgg cggcgctggg cgaagtgcct gacgccgtgc tgtgggtggc cggggagcgg
     1141 cttgtctcgg atcgtgggcc agacatggag gcgctgctgc gcgaggctgg gctgggcgag
     1201 cggctccgtc tgctgggtta tcgcgaggat gtgcccgcgc tgatggcggc cgcggatatt
     1261 ttcgtgttgc cgagccggtt cgagggactg ccgatgtcgg tgatcgaggc gatgctgacc
     1321 gtgctgccgg tggtggcgtg cgatgtgcgc gggccggcgg agcaggtggt ggagggcgtc
     1381 acgggattgc tggtgcctgt tggcgacgcg cccgctttgg cgggtgcgct gcggcggttg
     1441 acggaggata cggagttgcg ggcgcgcatg ggcgccgcgg ggcgggcgcg ggcgatcgag
     1501 cgctatgacg aggcgaaggt gctcgcaagg acgatcgaac tgctggaatt gtagcccagc
     1561 gccgcttgag ccaggacttc ccccggtcta tcccgccccg accggacgga ggccgccatg
     1621 ccagacacaa ttccaatccg caccgcgctg atctcggtct ccgacaagtc cgggctggtg
     1681 cccttggcgc aggccttggt ggcgcaggga gcgcggatca tatcgaccgg cggctcggcg
     1741 cgcgcgttgc gcgacgcagg ggtgccggtg accgaggttt cggaacacag cggctttccg
     1801 gagatccttg atgggcgggt gaagacgctg gtgccgcaga tccatggcgg catcctcggg
     1861 cgccgcgatc tgccggagca cgtggcgcag atggtgcagc acggcatcgc gccgatcgat
     1921 atggtggcgg taaacctgta tccgttcgag gggacggtgg cgcgtggcgc ccgttatgac
     1981 gagtgcgtcg agaatatcga cattggtggc ccagcgctga tccgggcagc ggccaagaac
     2041 cacgacttcg tcactgtgct gacggagccg gagcagtacg ccgaggttct ggaggagatc
     2101 gcgcgatcgg gtggcacctc actggcgttg cggcggcggt tggcgggggc cgcgtatgca
     2161 cgcaccgccg cctatgacgc ggcgatcgca gcgtggttcg cggagcagcg cggcgatact
     2221 ttccccgcgc ggctgacgct tgccgggacg ctgcggcaga cgctgcgtta tggcgagaat
     2281 ccacatcaat cggcggcgtt ctatgtgtcg ggtgatcggc tggggattgc gacggcggcg
     2341 caggtgggcg ggaaggagct gtcctacaac aacctgaacg ataccgacgc ggcgttcgaa
     2401 tgcgtcgcgg aattcgaggc gccgacggtg gcgatcatca agcatgccaa tccgtgcggt
     2461 gttgccagcg cagggacgct ggccgaggca tgggacctcg cgttgcgctg cgatccggtg
     2521 tcggcatttg gtggcatcgt cgcggtgaat cgcgtgctgg acacggcggc ggcggagaag
     2581 atcgcctcga tcatgaccga ggtgatcatc gcaccggatg ccgatccggg tgccaaggcg
     2641 gcgctggcac ggcgcaggaa cctgcggctg ctgctgaccg ggggcgtgcc ggatgcggcg
     2701 gcgggcggac tgacgtttcg cagtgttgcc ggcgggttcc tggcgcagac gcgggacaat
     2761 gggcgagtgg ggcggggtga tctgaaggtg gtgacgcagc gcgcgccaac cgaggcggaa
     2821 ctggcggatc tgttgttcgc attcagggtg tgcaagcacg tgaagtcgaa cgctatcgtc
     2881 tatgcgaagg atgcggcgac agtggggatt ggcgccgggc agccgagccg cgtggacagc
     2941 gcgcggattg gggcgtggaa gagcgaggag gcggcgaagg cggcgggact cgatgtgctg
     3001 ctggcgcggg gcagcgtggc ggcgtcggat gcgttctttc cgttcgcgga tgggctggag
     3061 gcggcgatcg cggttggcgc tacggcagtg atccagcctg gagggtcagt gcgggacaag
     3121 gaggtgatcg cggccgcgga cgccgcgggg atcgcgatgg tgttgaccgg gatgcggcac
     3181 tttcggcact gacccagtgt cattgcgaga gacggagcga cgaagcaagc caggacggga
     3241 ttgcttcgtc ggcttcgcct gttcgcaatg acaattgggg ttatttcaca tcgctgtacg
     3301 gatacatgag ggttccagtc ttcaggtccg ttggccacag aattacttca gtgttcgcgt
     3361 ccttgaactg gtttacgtcg ttcgccttca cattctggaa ctgcacttcc agcacgcggg
     3421 cttcggacca ttcgccatca gggccgaact tcacgtcgcc caccactgtc ttgaacgtgt
     3481 gcgagcggag gtaatcggcc agcttgtcct gatcgaggct cttcgtgccc tctaccgcct
     3541 gctggaggac ctgcatgtag ccataggcga atggcggcag ataatagccg agcgggtcga
     3601 cacccgccgc gccggccttc gcctgatatt gttcgaggaa tgccttcgcc tcgggcgatg
     3661 ccgatttcga ccagggcagc cagaagtcgt agtcgacgat gccgttgagg agcggaccga
     3721 gctgcgcctt gatcgcggtg ctttggaggc cgaccatgcc accaccgaac agcttcgcct
     3781 tcaggccgac ttcatgagcg gcgcgaatca tgccgaccgt atcgggaggg tacgaacaca
     3841 cgagcacgag gtccggattg gttgcctgga tcgctcgcac gattggggtg tagtccgtcg
     3901 tcgtgggggg atacgtcttg tcgtagacga ccttcagacc ttccttcttc gcgatcgccc
     3961 gcgcgccatc catcgcgttg tgcggaaatt cggcgtcggc gccgacgatc gccagggtct
     4021 gcggcttcgg gttctgtgcc atcgctgtgg cgaagaagcc cgcggcgaag ctctgcttcg
     4081 gttccgggcc gccggtcggc gtgaacgaga aatacttcgg atagtggaat tcggtgttcg
     4141 cggcgaggcc gagcaggctg aggaaggtgc ggttatgctg catgatgatc ggcatcgcag
     4201 gcgcgatcat attggtggca tagccgctca ccacgaggtc gaccttatcg acgtccagca
     4261 gcttcgtgta cagacccggg atcgtcgcag gattcgactg gtcatcgtaa tagaccagct
     4321 tcaccggtcg cccgagcagt ccgcccttgg cgttgatgtc ggattcccag atctgcatcg
     4381 ccagcagcgc ggctttgccg ttgggcgcga ggccgccggt gagcgccatg ccgaaaccga
     4441 tggtgatcgg ctcggcggcg tgtgctgctc cgagcgacgc aacggcgaag gcagcggcga
     4501 gagcgagacg cctcagcatc cgtgtttcct cctgttgatt ggttgggcgg catcctattg
     4561 ggtgagggtt ggttgcgcca gtgggtcgag gctcgcgtcg ctttagctct ggtcctcgac
     4621 ggttcgcggt gtaaacacat gcgcatggta tttgatccgg aagcagtccg cgccttcgaa
     4681 cgctcgcgct ggcaacaggc cgcgcgggcg tacgagacat cgttcgcgac ggccaccacg
     4741 ccattcatcg atgcgctgct cgattccgcc ggcgtcgccg ctggcacacg gatgctggat
     4801 atcgcctgcg ggccgggtgt ggtggcggcg caggcagttg cgcggggcgc ggtggcgcgc
     4861 ggtctggatt tctcccctgc catgctggcc atcgccatcg accgcggcat tgcggcagat
     4921 gagggcgatg ccgaggcctt gccgtatatg gacgccagct ttgatgcggt tgtctcgaat
     4981 ttcggcgtgc atcatgtgcc gcggcccgcg ctcgcgctga gcgaggcgca ccgcgtgctg
     5041 agcgccagag ggcaggtggc attcagcttc tgggccgagc acgccgagaa tattgcctgg
     5101 aagctggtgt tcgatgcggt ggcgcggcat ggcgatccgg ctacctcgga tgcgccacca
     5161 cccggaggtg gatttcgcgg tgccgcgcaa tgcgcggagg cgttgcgtgc ggcggggttc
     5221 gtcgattgca cgacgcaatt ggtccatgcg acgtggcgcc atgcggacgc agcagagttg
     5281 ctggatgcgc tacagacagg gacggcgcgc atggcggcga tgatcgatgc gcagccgcgg
     5341 gcggcgatac cggcgatcat tgccgacatc gaggataacg cggcgcgtta ccgcacttcg
     5401 gatgggatcg cgatacccat cgctgcggtg atcgccgccg gaacgaagag ctgaaggagg
     5461 gccagctgaa catctatcat gcctggtgcg acctgaagcc tggcatctcc gataccggct
     5521 tcgccgacaa ggtagccgcc tatatgggcc acctgaagga acaggggctg atcgagggtt
     5581 ggcgcctgac gcgccgcaag ctgggacttg gcgggccggg gctcggcgaa ttccacctga
     5641 tgatcgaggt gaaggacctg gcacagctcg agcaggcgtt cgagcgcgtc gccggccggc
     5701 gcgagcctgt ggagggcttt catttcggtg tcaattcgct ggtccagaac gcggtgttcg
     5761 cattgtatcg tgactttccc gatacctttc gccaccgtgg cgaagagcga ttctgatccg
     5821 agagggaaac ttatcgaatg ctactgcttc gcgttctcag cctggctgga accgcattgc
     5881 tggtgggcgc ggccgcgccg gatctgccat cggtgacagg ccagtcaccg gtcgccgcgg
     5941 gcgaatacct ggtggcgatt ggcggatgca acgattgcca taccgaaggc tggatgatga
     6001 atccgggcaa ggtgccggct gcgcaacggc tgacaggcag tcatgtggga tggaacggtc
     6061 cgtgggggac gtcctatcca accaacctgc gtctgctggt gcagaagatg tcagccgacg
     6121 cctgggtgca gcatgcgcat accatgcagc cacggccgcc catgccgtgg tacaatatgc
     6181 gttcgatgag cgagccggat ctgaaagcga tgtatgtgta tattcgcagc ctcggtccgg
     6241 ccggggaacc ggcgcccgac gcgctgccgc ccgggcagaa gccggcgacc tcgtatatac
     6301 gagctgagcc ggtgccgccg cagaagggtt agtggaagac gcctggccgg gtcaggtcgt
     6361 tccaggttgc caggatgaac agacctagca gcagcgccag gccggcacgg aacccgtact
     6421 cttgcacgcg cgggggtatg gggcggccac ggagtgcttc ggcgagatag aacagcaggt
     6481 ggccgccatc gagcaccgga atcgggaaca ggttgagcag gccgaggttc accgacagca
     6541 aggcgatgaa ttggatcagg tagagcggtc cttgctccgc cactgcaccg gataattgtg
     6601 cgatgcgcag cggtccaccg agatcttcgg tgccacgctt gcctgtgatc atctgccata
     6661 caccgacgat ggtctcccgc gtgatgtccc aggtctgggt cacaccgcca gccagcgctg
     6721 ccggcagtga cagccgctgg tactcgatct ggccaccgct gatgccgagc aagccgactt
     6781 tggtgtcacc cgacatgtgc gagtcagtca ccaccggcag tgttagcgtg gtgtcgccac
     6841 ggcggaccgt cagggtcaac ttctcggagg gatgcgccga cacgatggtc tggatatcgc
     6901 caaagctgct ggtgggtttg ccgtcgattg cgatgatgcg gtcgttgacc tgcagtccgg
     6961 cgtgtgccgc ggcgctgtct ggcaggacgc cgccggccac cggcaggatg gtgggcttgc
     7021 cggccgtggc gaacagtgca gcgaacagca ctatggcaag caggaagttg gcgattggtc
     7081 ctgcggcgac gacgatggca cgcgagccga cacgcttttc atggaagagg cggccgggca
     7141 tccaggtggc gcggacgtcg tccgatacgt cctcggcccg ctcctggccg tgcaacttca
     7201 cgtagccgcc caacggcagc caggcgagtt tccaaaccgt gccgacccga tcgcgccacg
     7261 tcgcgatcgc ggggccgaag ccaatggaga agacctcgac atgaacgcca cgccagcgtg
     7321 ccgcgatgta gtggccgaac tcgtgcacga aaaccagcac gccgagcacg acgatgaagg
     7381 cggcggcgct gcgaagcaga tcaagcattg gctgaaggac tttcgggtct aaaccggcga
     7441 gtcacgcggc gcgcgccgag gtcaaacgtt ccgcagtgcg acgggcagcg gcgtccactg
     7501 cgaccacctc gtcgagcgta tcggctgaat gggtgcccat tgcgtccagc acctccgcta
     7561 cagtgccggc gatgtcgagg aaacctatcc tgcgttgcat gaaggcttcc acggcaattt
     7621 cgttcgctgc actgaggata gctggagcgc cgccgccttc gcgcaaggcc tcgcgcgcga
     7681 gacgcaaagc agggaagcgt tcggtgtcgg gtggcatgaa gtcgagccgc gccagtgtag
     7741 cgagatcgag ccggggcgac ggcgtgctca tgcgttctgg ccatgccaat gtatgtgcga
     7801 tcggaatgcg catgtcgggt gagccgagtt gtgccagtac gctgccgtcg tggtagcaga
     7861 ccatgccgtg aatgactgat tgcgggtgaa ccagcacgtc gatttggggt tcctgcaggc
     7921 cgaacagtcg ggcggcttcg atcagctcaa ggcctttgtt catcaacgtg gccgaatcga
     7981 tgctgatctt ggctcccatg gaccagacgg ggtgcttgcg cgccatgtcg ggggtcgcgg
     8041 cggccatctc gtcgcgtgtc gcggtgcgga acgggccgcc ggatgcggtg agcacgattt
     8101 tttcgaccgc gtgacggttt tcgtcctcga gcacctggaa gatcgcgttg tgctcggagt
     8161 cgaccggcaa caatgtggcg ccggcttcct tgaccgcctg cagcatgacg tcaccggcgc
     8221 agaccagtgc ctccttgttg gccagagcca ccacggcgcc gcggcggatg gcagcgagcg
     8281 ttgcctcgag gccggcggcg ccggtgatcg cggccatggt ccagtcggcc gccagtgcgg
     8341 cggcttcaac cacggcttcc gcgccagcgg cggtgtcgat gcccgtgccg tccagtgcgg
     8401 cgcggagttc catgtagcag gaagcgtcgg cgatcacggc ccggttggcc ttcagggcga
     8461 tggcctgctg cgccaacaag gttgcgttgc ggccggccac cagggcgcgg acctggaagc
     8521 gctggggttc ggcgatgagc aactcgaccg tctgggtgcc gacactgccg gtgctgccga
     8581 ggacggtgac actgcgcgga gctgaagtca ttgccacaaa actacaccac gaccgagggc
     8641 cagcgccagc agggccgcca cgggagctgc tgccagaacg ccgtcgagac gatcgaacag
     8701 gccaccatgg ccgggaataa gattgcctga atccttcact gcgagcagcc gcttcacgaa
     8761 gctttcgagc aaatcgcccg cctgggcaac ggtgctcagg acgacggcga tcagcgccgg
     8821 ttgccaaggc gtgacgggcg tcgccaggaa atgggcggtg aggagccctg tcgcaacggc
     8881 cgcgatcagg ccgccgccgg cgcccgacca agtcttgccg ggggaaatat gcggcgccag
     8941 gcgtgggccg ccgatgcagc ggccaacgac ataggcgccg atatcgccgg cccagacgat
     9001 gagcaggacg aagagcacat cggcgcggcc tgagacggga tcggcgcgga gccatagcag
     9061 ggaggtgccg gcgatcgcga catagagtag cccggcggag aacaggccgg gacgggttgg
     9121 cggcaggcat gcccaagccg gcctcggttc gatccgcggc gcttggggac ggaacaagag
     9181 cagccactcg ataaccaggc ctgtcgcgat gatgccgacg atgaggataa atgccactcc
     9241 gccgagccag acactggcga caccgatcgg ggtcaggaca gcggccgaaa ggatgcggat
     9301 cctcaggtcg ctccagcgtc ccggttgatg cttagccagg tcgggcaccg aatcgccgct
     9361 cacgccgcgc atattccgcc agcgcggccg cgaagtgctc cgccccgaag tccggccaca
     9421 gcacgtcgag gaacactagt tctgaatagg ccgcctgcca cagcaggaag ttcgacagcc
     9481 gctgctcgcc ggaagtgcgg atgatcaggt cgggatcagg gatgcccgcg gtggccaggg
     9541 agccggcgag catcgactcg tcgatctgtc cgggctcgag gcgtcccgac cgggcggcgt
     9601 cggccagtgc acgggctgct tcgacgatct cggcccgggc gccgtaggac agggcgacaa
     9661 tcaaattcag gcgcgtgttg ctcgcagtat cccgctcggc attcgtcaga tccgcgccga
     9721 tatcgggatc gaatttcgct cgatcgccga tgaagtggag ccgcacgccg ttggccttca
     9781 actcggcaat ttcgctcttc aggtactggc ggagcagtcc cgtcaggtcc agtacctcgc
     9841 ccgccggacg gcgccagttt tcgctgctga acgcataaat ggtcagccag gccacgccat
     9901 tttcaattgc cgcctggatg gttcgccgga cggcgcgcgc gccctcgcgg tggccggcaa
     9961 tctttggcag gtggcgggac gcggcccagc ggccgttgcc atccatgatg atcgcgacat
    10021 gggacgggat ggacaccaat cagacctgct tgatgtcctt ttccttgtcg gccaacgagt
    10081 cgtcgacgcg cttgatgtac tgatccgtca gcttctgcac ctcggcgctc cagtccttgg
    10141 cgacgtcctc gccgatgtcg tgctttttct catgcgtctt gatctgttcc ataccatcgc
    10201 ggcgaacacc gcgaacggcc accttggcgc cctctgcata tttgtgcgct gctttcacca
    10261 gttcggtgcg gcgctcagtg gtgagctggg gaatcgggac ccgaaccaac tgcccgtcgg
    10321 cagcagggtt cagcccaagc ccagcgtcgc ggatggcttt ctcgaccgcg ccgaccaggc
    10381 tgcggtccca cacctgcacg gtcagcatcc ggggctcagg tgcgccgaca gtgccgcact
    10441 gggtcagcgg catctcggtc ccataggcct ggaccttcac cggctccagc agcccgggat
    10501 gggcgcggcc ggtgcgcagc ccgttgaatt cgcgcttcag cgtgtccagc gcgccatcca
    10561 tacggcgtgt aaggtcctgt ttcagcgtgt cgacatcggc ttgtggcatg gcgccctccc
    10621 agacctgtcc gggccattct tattgcagtt cgatgatctt ggtgaagcgg ccttcgccgc
    10681 ggatcacagc cgcgaaggcg cccggagcgt gaatattgaa caccacgatc ggaagcctgt
    10741 tctcccgcgc caggctgatc gcggccgcgt ccatcactgc cagatcgttc gcgagcacgt
    10801 cgagataagt aagctgctca tagcgggtgg cgccgctgac cttgcgggga tcggcggaat
    10861 agacgccgtc aacctgggtg cccttgaaca gcgcctggca ctgcatctcg ttggcgcgca
    10921 gggccgccgc ggtgtcggtg gtgaaatagg gatttccggt gccggcggcg aaaatcacca
    10981 cacggccctt ctccatgtgg cgctcggcgc ggcggcggat gtagggctcg cagaccgagg
    11041 acataggtat agccgactgc acccgcgtcg gcacgccgat tttctcgagc gcggactgca
    11101 tggcgagcgc gttcatgaca gtggcgagca tgcccatgta gtcggcctga gcgcgctcca
    11161 tgcccgaggc cgcgccggag actccgcgga agatattccc accgccgatg acgatgcaga
    11221 cctgcacgcc catggcaacc acgtctctga cgtcggtcgc gatggcgcgg acggtctcgt
    11281 tgtccagccc gtattcccgg cggcccatca gggcctctcc ggagagtttc agcaggacgc
    11341 gctggtagac cggttggtct ggcatggcga gcggcccccc tgacgatggc ggcggcaccc
    11401 tactggcagg gcccgcggtg aacaagttcc cggcgcgtcg cgaaacttga aggaatgttg
    11461 aaacccctcc caggctggtg attgccagct atgcatggcc ggcgcccgcg acagggcttg
    11521 caccgacagt ctccgtgacc gcgcgtccgg cgagcgggcg gctgctcgcg tcatttctca
    11581 ccgacctgat tgcatcagtg gtgatcgtgt tgatgctcgg ctggctggtc caggcctcgg
    11641 ctggcaacga ctacctacgc gacgccatag tcgcccagaa cgacatctac gtttcgatca
    11701 gccggttcac gccgcacaac ctgggcatga gctacgtgag cacggtggtc caaatgaacc
    11761 gaggcgtggg cttcggctac cagccggggg agggctacac cgatgtgatc caatacgggc
    11821 tggtcaccgt ggcgattgtg atcctaaacg tctttcgtgc cctgccggat accatcctgg
    11881 agttgtacca cgagaccagc ggcacggctg cctggatcgt gctgggcggc ttcgcggtag
    11941 gggttggcag cgtttccgtt gctctgctct ctatgcgcag ttccccctgg cggctggtcc
    12001 tggcgatcct ggcgagccct ttcgccattt cgctgatatt cctggctttg cagggcttta
    12061 tgatagcgat gatggacaca ttctactggt tcacttcact tgcgccgtac acagtcgcat
    12121 gtccggtgat ctgcacgttg tactgggttg catttccgaa tgccgaacgc ggcgccacag
    12181 cgaccctggc gcaagccatg ctgcgggtgc tggacccgaa gcgctagctt gatggccatc
    12241 gcggctacgt tgttttaccg catcagtcgg acgatagccg tgactacatc cagcacgtcg
    12301 atcagtcgcg ccacgtagcg gtccagcacg gagtagcccg gcgccgcggg atccagcatt
    12361 tgccgctcca tggcgtctat catgcgccta agccgtcgct gatgcaggcc gattcgtcgc
    12421 tgcaccggat cagtaacgat cccggcgaac gctgacagca ctgctccgat catcagcagc
    12481 ccaccgctca atccaacggc cagcagcggt gcggccgatg caggatacat tccgtaccaa
    12541 agtgatccga gcccggcgcc cagcgggaac gatgagatcg c
//
LOCUS       DATGEM010000177         8651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1107793, whole genome shotgun sequence.
ACCESSION   DATGEM010000177 DATGEM010000000
VERSION     DATGEM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107793"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VKQ27_05600"
     CDS             complement(<1..835)
                     /locus_tag="VKQ27_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLY88440.1"
                     /translation="MGRPHLDLTRITLSVLFIGSLIVACVWIMLPFLAAIIWALTLVI
                     ATWPLMLTVQRYTGHRRGIAVLIMTLVLLMLLIVPCWLAVGTIVANTDQIADMARTVV
                     SLRVPPPPDWVAALPLIGDRLAHIWGDLTSLGVNELAPKLTPYAGTLTRWLVGAMGSV
                     GEMFLQFLLTVAIAAVMYANGERAADTVLRFGRRLGGERGEGAVRLAGQAIRGVALGV
                     VVTALAQSVLGGIGLAVAGVPFATVLTALMFILCLAQLGPALVLIPAVIWMYYSGSAL
                     WA"
     gene            910..1053
                     /locus_tag="VKQ27_05605"
     CDS             910..1053
                     /locus_tag="VKQ27_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88441.1"
                     /translation="MARADPTLLVTVRSPDYSLAPMPPPAIQSADAQRFRAYFPTIEL
                     ITP"
     gene            complement(1043..2749)
                     /locus_tag="VKQ27_05610"
     CDS             complement(1043..2749)
                     /locus_tag="VKQ27_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLY88442.1"
                     /translation="MDEEPASKRGLARNTANYGDRDFALYLRRSFAKSMGYSQAMLDR
                     PVVGIVDTGSGFNNCHRTVPELIEAVKRGVLAAGGLPLPFPTVSLCEPSLFPTSMHYR
                     NLAALDTEAMLTAQPMDAAVLIGGCDKTVPAQLMAAASADVPAVQLVTGPMLATPFQG
                     ERLSACTDCRRYWAMYRAGRVSAERIGEIEGRLATTAGTCGVMGTASTMACIAETLGM
                     VPLGYGSIPAVHADRLRAAEEAGALAVKLIAAPIRPSEIITAASVENALRVLLALGGS
                     TNALIHLTAIAGRRGVKIDLHRLNDLSDNTPVLVDLKPTGEGYMEDFHAAGGMPALLW
                     ELRDLLHLETRDVTGTTLKERLREPVFVDRRYIRARGEPVSPVGGLVALFGSLAPRGA
                     ILKRSAATPALFETEARAVVFDGLEDLASRIDDPALDVTEQDILVLKNAGSLTPAGMR
                     EAGYLPIPQKLARAGVKDMVRMSDCRMSGTAFGTIVLHITPEAAAGGPLGLVESGDRI
                     RLSVKERKLELLVADDVLTKRRASWCPPAKASRGWDRIIQEQVLQADEGCDLAVLRGA
                     TV"
     gene            2809..3867
                     /locus_tag="VKQ27_05615"
     CDS             2809..3867
                     /locus_tag="VKQ27_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88443.1"
                     /translation="MAVNLKRLVYFDSFMDPIADTIIGKRDDIDLVRLEYDSPEPDNW
                     PEISRAHGYQIQPRGELRDPWFGDAALLARCPNMLAISSTGAGYDMIDVDACTAAGVI
                     VVNQSGTNWEPVAEHALGLMLSLTKKIALSERAMKRTPNLDRREYAGNNLRNKTVGII
                     GIGHIGRRVAQLCGVLFNNTVLAYDPYLTAGQIAERGATKVELDELLARSDYVTVHCP
                     RSGETFGMFGAPQFTAMKPSAYFINTARGGIHDEDALADALCAGKIAGAGLDVFLKEP
                     PDPSHPLLNMDNVIASPHIAGMTVETMTEMCEATALQWLTIFDGGVPPRLVNPEVWPR
                     YAERFAELLGFRPADMGG"
     gene            3960..4541
                     /locus_tag="VKQ27_05620"
     CDS             3960..4541
                     /locus_tag="VKQ27_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88444.1"
                     /translation="MFRCLLLCVAVATLAGCGPSYSPNTYATDAVQQAAKVDQGVIVG
                     VRNVDISASGTTGAVVGGAAGGIAGSQVGEGAQKALTTLGSTLVGGIAGSSAEHVVSD
                     TTAFEYIVREPKGDLVSVTQKDRVPLAIGQKVLVIAGKQARVVPDYTVAVDSDAKDKP
                     ADPSRQASAAVPPPPPPVQAMPLAPPANAETGP"
     gene            4627..5388
                     /locus_tag="VKQ27_05625"
     CDS             4627..5388
                     /locus_tag="VKQ27_05625"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLY88445.1"
                     /translation="MSAALQLHRTDMSDAEWQARCELAALYHVVDYLGWTDLINTHMS
                     ARIPDDPDTFLINNYGEMFDEITASSLVKMDMDGRVYSDGGKFNNAGFTIHSGVYKAR
                     PDAMCVMHTHTRAGAGLSLLKKGLRPISQDALQVYDDIAYHGYGVPASPEECEALGRT
                     CKNGSHVVLMNHGLLTFGPTVHGAFYRMYCLERACELEVIARQLNAEPATIDEYVIGK
                     AAERMRKRRDTSEYGHAEWQGLVRTVDRKGADYRR"
     gene            5412..5969
                     /locus_tag="VKQ27_05630"
     CDS             5412..5969
                     /locus_tag="VKQ27_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88446.1"
                     /translation="MPFDALLAPTRHRSLAELLDEHGATPVPLDLLAAHKRAQLERFA
                     PSFWHQHQTWLPIGLVGSVGCMAASGGLANHLVPGSLLPSWLSLGWLSLIAMLILFGV
                     FRVSAGASWQERTVVPELLGQVGVPPQIARLARMLHRDALHATLLLGELVREEVVLDP
                     YLLMECDGEWVCLGIWDGTTIVAAA"
     gene            6061..7104
                     /locus_tag="VKQ27_05635"
     CDS             6061..7104
                     /locus_tag="VKQ27_05635"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HLY88447.1"
                     /translation="MDGMSRSADEHLVTFDLLTENPVYKPFRYPWAYEAWLIQQRVHW
                     LPEEVPLADDVKDWHRNLSASERNLLSQIFRFFTQADVEVNNCYMKHYARVFKPTEVL
                     MMLSAFSNIETVHIAAYSHLLDTIGMPELEYQAFLKYKEMKDKYDFMQSFTVDSRYEI
                     AKTLAAFGAFTEGLQLFASFAILLNFPRFGKMKGMGQIISWSVRDETLHCNSIIRLFR
                     TFVQENPSIWTEELRRDIYLTCATIVDHEDAFIDLAFEQGAVEGLTSQEVKTYIRYIA
                     DRRLMQLGLNPLFHVAKNPLPWLDEMLNAVEHANFFENRATEYSKASTTGSWEEAFAS
                     PQSDSDGVPLAPA"
     gene            7411..7692
                     /locus_tag="VKQ27_05640"
     CDS             7411..7692
                     /locus_tag="VKQ27_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88448.1"
                     /translation="MSQTDEGAGREVGPGGAPRYTRRLSDKILIAFHHACDQADFEVA
                     EQLLHILEMMLTRRPLTPDGTRRRNMESLVAAHERLWHLRHPHTGDDSF"
     gene            complement(7705..8436)
                     /locus_tag="VKQ27_05645"
     CDS             complement(7705..8436)
                     /locus_tag="VKQ27_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLY88449.1"
                     /translation="MSVSTITHGHLLAGRVAYAQLSAGFRSGIEPVLLAAAVPARPGD
                     RVLEAGSGAGATLLCLMARVAGVQATGIEKSEQLTRLARQNAEANGWPAMCLIAADIA
                     EAGFADTFDHACANPPYHPADGSASPDASRDSAKRGSPGLLAVWARALSRSLKPRGTL
                     TLILPTAALPRAVTALADSGCAPTALLPLWPKLGRPAKLMLLRSVKGGRSPFRVLPGL
                     VLHTSGGDYTPETDAVLRDGAALAI"
     gene            complement(8446..>8651)
                     /locus_tag="VKQ27_05650"
     CDS             complement(8446..>8651)
                     /locus_tag="VKQ27_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88450.1"
                     /translation="EVQHCPQVDDPQHQMVQLRDPQHGHLSKRWTREFSLLPGRRQVP
                     AAGFSLQALTSPVIQLTRPIGFA"
BASE COUNT         1602 a         2912 c         2659 g         1478 t
ORIGIN      
        1 tcgcccataa cgctgaaccc gagtagtaca tccagatcac cgctgggatc aacacgaggg
       61 ccggcccgag ttgtgccagg cacaggatga acatcagcgc ggtgagtaca gtggcgaacg
      121 gcacgcccgc caccgccagg ccaatgccac caagcacgct ctgcgccagc gcggtcacca
      181 ccacgcccaa cgcgacgcca cgaatcgcct gtccggcgag ccgcactgca ccctcgcccc
      241 gctcgccacc cagccgtctc ccgaagcgca gcaccgtatc cgctgcgcgc tcgccattgg
      301 catacatcac cgcggctatt gccacggtca gcaggaactg caggaacatc tcaccgacgc
      361 tgcccatcgc gccgaccaac cagcgggtga gcgtgccggc gtacggcgtc aactttggcg
      421 cgagctcgtt cacgccgagc gaggtgagat caccccagat gtgggcaagt cgatcaccga
      481 tgagcggcag ggcggctacc cagtcaggcg gaggtggcac gcgcagggag acaacggtgc
      541 gtgccatgtc ggcgatctgg tcggtgttgg cgacgatggt gccgaccgcc agccagcacg
      601 gcacgatcag cagcatcagc agcacgaggg tcatgatcag cacggcgatg ccacggcggt
      661 gaccggtgta gcgctgcacg gtcagcatca acggccacgt cgcgatcacc agggtcagcg
      721 cccagatgat cgctgccagg aacggaagca tgatccagac acaggcgacg atgaggctgc
      781 cgatgaacag gaccgacagt gttatgcgtg tgaggtccag atgtggtcgt cccatgccgc
      841 ctggatcccc cgtcccctct ggcgcaagcc aaccctgcga tgcgcctact cggattgcaa
      901 tgctacggaa tggcacgcgc cgaccctacc ttactggtga cggtgcgctc gccggattat
      961 tctttggcac ccatgccacc gccggcgatt caatctgctg acgcgcagcg ctttcgcgcc
     1021 tacttcccga cgatcgaact gatcacaccg tagcgccgcg cagcaccgcc agatcgcatc
     1081 cctcatcggc ctggagcacc tgctcctgga taatgcggtc ccagccccgc gatgccttcg
     1141 caggggggca ccaacttgcg cgtcgcttcg tcagcacgtc atccgccacc agcagctcca
     1201 gtttccgctc cttcaccgac agccggatgc ggtcgccgct ttccaccagc cccaacggcc
     1261 cacctgccgc cgcttccggc gtgatgtgca gcacgatggt gccgaacgca gtgccggaca
     1321 tgcggcaatc gctcatcctc accatgtcct tcacgccagc gcgcgccagc ttctgcggaa
     1381 tgggcagata gcccgcttcc cgcatgccgg cgggcgtcag cgatccggca ttcttcagca
     1441 ccagaatatc ctgctcggtc acatccagcg cgggatcatc gatgcggctc gccagatctt
     1501 cgagcccatc gaacaccact gcccgcgcct cggtctcgaa cagcgccggc gtcgcggcgg
     1561 agcgcttcag gatcgcaccg cgcggcgcga gcgacccgaa cagcgccacc agaccgccca
     1621 ccggcgacac cggctcgccg cgcgcccgta tataccgccg gtcgacgaac accggctcgc
     1681 gcagccgctc cttcagcgtc gtgccggtga catcgcgcgt ctccagatgc agcaggtcgc
     1741 gcagctccca caacagtgcc ggcatgccgc ccgcagcgtg gaaatcctcc atgtagcctt
     1801 cgccagtggg cttcaggtcc accagcaccg gcgtgttatc cgataggtca ttcaaccgat
     1861 gcagatcgat cttgacgccc cgccttccgg caattgccgt cagatggatc agcgcgtttg
     1921 tcgacccgcc cagtgccagc agcacgcgca gcgcgttctc caccgatgcc gcggtgataa
     1981 tctcggacgg gcggattggc gcggcgatca gcttcaccgc cagcgcgccg gcttcctcgg
     2041 ctgctcgcag ccggtcggcg tgcaccgccg ggatcgaacc atagcccagg ggtaccattc
     2101 ccagtgtttc ggcaatgcag gccatcgtgc tcgccgtacc cataacgccg catgttccgg
     2161 cggtggtcgc cagccgtccc tcgatctcgc cgatccgttc ggcagagacg cggccggcgc
     2221 gatacatcgc ccaatagcga cggcaatcgg tgcaggcgga cagccgttcg ccctggaacg
     2281 gcgtcgccag catcggtccg gtcaccagtt gcaccgccgg cacatccgcc gatgccgccg
     2341 ccatcaattg cgccggcacc gtcttgtcgc agccgccaat cagcaccgcg gcatccatcg
     2401 gctgcgcggt cagcatcgcc tcggtgtcca gcgcggcgag gttccggtag tgcatcgagg
     2461 tggggaacag cgacggctcg cacagcgaca cggtggggaa gggcagggga agcccgccgg
     2521 cagccagcac gccgcgcttc accgcctcga tcagctccgg taccgtgcgg tggcagttgt
     2581 tgaacccgga accggtgtcg acgatgccca ccacaggccg atcgagcatc gcctgcgaat
     2641 accccatcga cttggcgaac gatcggcgca gatacaatgc gaagtcgcga tcgccatagt
     2701 tggcggtgtt gcgggccaag ccgcgcttcg atgcgggttc ttcatccatg cggaagatgc
     2761 tacgccactg gcaactgacg agcaatttcg gagcggggag agacgacagt ggcagtgaac
     2821 ctgaagcgcc tggtctattt cgatagcttc atggacccga tcgccgacac tatcatcggc
     2881 aagcgtgacg acatcgatct ggttcgcctg gagtacgact ctcccgagcc ggacaactgg
     2941 ccggagatca gccgggcaca cggctaccag atccagccgc gcggcgaatt gcgtgatccc
     3001 tggttcggcg acgcggcgct cctcgcgcgc tgccccaaca tgctggcgat cagctccacc
     3061 ggcgccggct atgacatgat cgacgtcgat gcctgcaccg ccgccggcgt gatcgtggtg
     3121 aaccagtcag gcaccaactg ggaaccggtc gccgagcacg ccctcgggct gatgctgtct
     3181 ctgactaaga agatcgcgct gtcggaacgg gcgatgaagc gcacgcccaa cctcgatcgt
     3241 cgcgaatacg ccggcaataa cctgcgcaat aagaccgtcg gtatcatcgg catcggccat
     3301 atcggccgcc gcgtcgctca gctttgtggc gtgctattca acaacacggt actggcttac
     3361 gacccctacc tcaccgccgg gcagatcgcc gaacgtggcg ccacaaaggt ggagctggac
     3421 gagctgctcg cgcgctccga ctacgtcacc gtgcattgtc cacgtagcgg cgagaccttc
     3481 ggcatgttcg gcgcccctca gttcaccgcg atgaagccaa gtgcctattt catcaacacc
     3541 gcgcgcggcg gaatccacga cgaagacgcg cttgccgacg cactgtgtgc cgggaagatc
     3601 gcaggggcgg ggctcgacgt gttcctcaag gagccacctg atccttcgca tccgctgctc
     3661 aacatggaca acgtaatcgc cagcccgcac atagccggaa tgacggtcga gaccatgacc
     3721 gagatgtgcg aggcgactgc gctccaatgg ctcaccattt tcgacggcgg cgtcccaccg
     3781 cggctggtca atccggaagt ctggccgcgc tacgccgaac gcttcgccga actgctcggg
     3841 ttccgaccag ccgatatggg agggtagccg gccagccgtc gggcagcatc tgcggctgtc
     3901 gtggcaggca ttcgcatccg cgatgacaat cgggggcgaa ttccgctaat tagcgccgca
     3961 tgtttcgctg cctcctcctc tgcgtcgccg tcgccacact tgctggctgt gggcctagct
     4021 attcccccaa cacctacgcc accgacgccg tccagcaggc ggcgaaggtg gaccagggtg
     4081 tcatcgttgg cgttcgcaac gtcgacatca gcgcctccgg cacgaccggt gcggtcgtcg
     4141 gtggtgccgc cggcggtatc gccggatcgc aggtgggcga gggcgcgcaa aaggccctta
     4201 ccaccctcgg cagcactctc gtcggcggca tcgccggcag ctcggctgaa cacgtcgtca
     4261 gcgacaccac ggctttcgaa tatatcgtgc gcgaaccaaa gggcgacctc gtcagtgtga
     4321 cgcaaaagga tcgcgtgccg ctggcaatcg gccagaaggt gctggtgatc gccggcaagc
     4381 aggcacgcgt ggtgcccgac tataccgtgg cggtggattc cgacgccaag gacaagccgg
     4441 ccgatccctc gaggcaagcc agcgccgcgg tgccgccccc accgccgccg gtgcaggcga
     4501 tgccgctcgc cccgccagcg aatgctgaaa ccgggccgtg atactgtccc gtgcaaccgt
     4561 aagggttgcg cggccacagc agttctctca ggatgcctgc cagaacgacg aacggagaac
     4621 gagccgatgt cagcagcact ccagctgcac cgcactgata tgtccgacgc cgaatggcag
     4681 gcgcgttgcg agcttgccgc actctatcac gtggtggact atctgggttg gacggacctg
     4741 atcaataccc acatgtcggc gcgcatcccc gatgatccgg acaccttcct gatcaacaac
     4801 tacggcgaga tgttcgacga gatcaccgcg tcgagcctag tcaagatgga catggacggc
     4861 cgcgtctata gcgacggcgg caagttcaac aatgccgggt tcaccatcca tagcggcgtc
     4921 tacaaggcgc gtcccgatgc gatgtgcgtc atgcacacgc acacccgtgc cggcgccggc
     4981 ctgtccttgc tcaagaaggg cctgcgcccg atcagccagg acgcgctgca ggtgtatgac
     5041 gacatcgcct atcacggcta cggcgtgcca gcctcgcccg aggaatgcga ggcgctgggc
     5101 cgcacctgca agaacggcag ccacgtcgtg ctgatgaacc acggcctgct gacctttggc
     5161 cccaccgtgc atggcgcatt ctaccgcatg tactgcctgg agcgggcgtg cgagctcgaa
     5221 gtgattgcgc gtcagctcaa tgctgagccg gcgacgatcg acgagtacgt gatcggcaag
     5281 gcggcggaac ggatgcgtaa gcgccgcgat acatctgaat atggccacgc cgaatggcag
     5341 gggctcgttc ggacggtcga tcgcaagggt gccgactacc ggcggtgata ccgtaacagg
     5401 aggaatgcgc catgccgttc gatgcactgc ttgctccaac caggcatcgc tcgctcgcgg
     5461 aactcctcga tgagcatggc gcgaccccgg tgccgctcga cctcctggcc gcgcacaagc
     5521 gtgctcagct cgagcggttc gcaccgagct tctggcacca gcaccagacc tggctgccga
     5581 tcgggttggt tggctctgtc ggctgcatgg cagcgagcgg cggcctggcc aaccatctgg
     5641 tacccgggtc gttgctgcct tcctggctca gcctcggctg gctcagcttg atcgccatgc
     5701 tgatcctgtt cggcgtgttt cgcgtcagtg cgggcgcgtc ctggcaggaa cgcaccgtcg
     5761 tgccggaact gcttggccaa gtcggcgttc cacctcagat cgcgcgcctg gcacgcatgc
     5821 tccatcgcga tgccctgcat gcgacgctgc tccttggcga actggtgcgc gaggaagtcg
     5881 tgctagaccc ctacctgctg atggagtgcg atggcgaatg ggtgtgcctc ggaatctggg
     5941 acggcacgac catcgttgcc gccgcgtagc tatcgctatc ttgcgcgacg gcccgctggg
     6001 agccacaata tctggtggaa cgggcgactc gcccctggca ccaccacgga gcaacgcagc
     6061 atggacggca tgtctcgctc ggccgacgaa cacctcgtca cgttcgacct gctgaccgag
     6121 aacccggtct acaagccgtt ccgttacccc tgggcttatg aggcatggct catccagcag
     6181 cgcgtgcatt ggctgcctga ggaggttccg ctcgcggatg acgtgaagga ctggcaccgc
     6241 aacctgagcg cgagtgagcg caacctgctg agccagatct tccgcttctt cacccaggcc
     6301 gatgtcgagg tgaacaactg ctacatgaag cactacgcgc gggtgttcaa acccaccgag
     6361 gtgctgatga tgctgtcagc cttctccaac atcgagactg tgcacatcgc cgcctacagc
     6421 catctgctcg ataccatcgg catgccggag ctcgagtacc aggccttcct caaatacaag
     6481 gagatgaagg acaaatacga cttcatgcag agcttcacgg tcgactcgcg ctatgagatc
     6541 gccaagacgc ttgcggcatt tggcgccttc accgaggggc tccagctgtt cgcctccttc
     6601 gcgatcctgc tgaacttccc gcgcttcggg aagatgaagg ggatgggcca gatcatctcc
     6661 tggtcggtgc gcgacgagac actgcactgc aactcgatca tccggctgtt ccgcaccttc
     6721 gtgcaggaga atccctcgat ctggaccgag gagctgaggc gggacatcta cctgacctgc
     6781 gccaccatcg tggaccacga ggacgcattc atcgacctcg cgttcgagca gggcgcggtc
     6841 gaaggcctga cctcccagga ggtgaagacc tacatccgct acatcgccga caggcgcctg
     6901 atgcaactcg ggctcaaccc gctgttccat gtggccaaga atccgctgcc ctggctggac
     6961 gagatgctga acgctgtcga gcatgcgaat ttcttcgaga accgcgccac tgagtacagc
     7021 aaggcctcca ccaccggcag ctgggaagag gcgttcgcca gcccgcagtc cgacagcgat
     7081 ggcgtgccgc ttgcccccgc gtaattgcaa actccgtccc aaaattggta ataatctgcc
     7141 caccacaaga cagactcttg tctgtgaagc gcatttgcac catattgtgt cgacggttcg
     7201 tgccgtgatt gctgacggtg tggctcagta acgcagtcgt tgcgcattaa tgacgtgcat
     7261 gcgcggctaa aagatgcaca cgctagtcta accgatccgt ccggctcgtt ttgcgtggca
     7321 gtatttgctc ggttggacgg attggctcga taatgccaac acctgcaaga tacgggatga
     7381 ggccagatga gtacgtcgac cgaggacaca atgtcgcaga ccgatgaagg cgctggccgc
     7441 gaagtcggac cgggcggtgc tccgcgctac acacggcggc tctcggacaa gattttgatc
     7501 gcctttcatc acgcctgtga tcaggcagat ttcgaggtgg ccgagcagct cctgcacatt
     7561 ttggagatga tgctgacccg ccgcccgctg acgcctgacg gcacgcgccg ccgcaacatg
     7621 gaaagtctgg ttgccgctca tgaacggctc tggcacctgc ggcatccgca caccggtgac
     7681 gactccttct aggtcggtag cggcctaaat cgccagcgcc gctccgtccc gcagcactgc
     7741 atcggtttcc ggcgtgtaat ccccgccgga ggtgtgcagc accagtcccg gcaggacccg
     7801 gaacggcgac cgcccgccct tgacgctgcg cagcagcatc agctttgcgg ggcggccgag
     7861 cttcggccat agcggcagga gagccgtcgg ggcgcagccg ctgtccgcaa gtgccgtgac
     7921 tgcccgtggc agtgccgccg tcggcaggat gagtgtcagg gtgccgcgtg gcttgaggga
     7981 ccggctcaac gctctggccc agacggcaag caaccccggc gaaccgcgtt tcgccgaatc
     8041 gcggctagcg tctggtgacg ccgagccgtc cgccgggtga tagggtggat tggcgcaggc
     8101 gtggtcgaac gtatcagcga accccgcttc ggcaatgtcg gcggcgatca ggcacatggc
     8161 gggccagccg tttgcctctg cgttctgtct tgcgagccgg gtcagctgct cgcttttttc
     8221 aatgcccgtt gcctgcacac cggctacccg cgccatcaga cagagcaagg tggcgccagc
     8281 gccgctccct gcctccagca cgcgatcgcc aggccgggcc ggcactgcgg cagcgagcag
     8341 caccggctcg atgccactgc gaaagcctgc gctcagctgt gcgtaggcga cccgcccggc
     8401 gagcagatgg ccgtgtgtga tcgttgagac tgacatgggc ggaacttaag cgaacccgat
     8461 gggccgcgtg agctggataa cgggtgatgt cagtgcctgt agagaaaaac ctgccgccgg
     8521 cacttgccgc cgtcctggca agaggctgaa ctcccgcgtc caccttttgg agaggtgacc
     8581 atgctgcgga tcacgcaact ggaccatctg gtgctgcggg tcgtcgacct gcgggcaatg
     8641 ctgcacttct a
//
LOCUS       DATGEM010000178         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1549734, whole genome shotgun sequence.
ACCESSION   DATGEM010000178 DATGEM010000000
VERSION     DATGEM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549734"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VKQ27_05655"
     CDS             complement(<1..595)
                     /locus_tag="VKQ27_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY88451.1"
                     /translation="MTFQAFRFDPVELPPAAKAFRKEVRAFLEENSASLGNRRDYDQQ
                     FSRRMAERGWIGMTWPKLYGGHERTAFERYVMIEEMLAAGAPVGFHWTADRQSGPNIL
                     RYGTDEQKDYFLPRIVRAELSFCIGMSEPDSGSDLAGTKTRAVKVDGGFRINGSKLWT
                     SNAHRADYMILFCKMASDGQEEVDRHAGATQFLVDLKN"
     gene            complement(668..1195)
                     /locus_tag="VKQ27_05660"
     CDS             complement(668..1195)
                     /locus_tag="VKQ27_05660"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HLY88452.1"
                     /translation="MLFRVCPTLLLLALAACGSSGPDGNYVGGTVALECAPFARALSG
                     VRLSGDAADWWYEADGQYLRSQAPAVGSVLVLRRSGRLPSGHVAVVSQVLGQREILVT
                     QANWVHHRVSEDQPVIDVSAANDWSLVRIWWPPTAQMGVSEYSAFGFIRSDRSSSHDE
                     LIAATPRAIRLAENE"
     gene            complement(1303..2484)
                     /locus_tag="VKQ27_05665"
     CDS             complement(1303..2484)
                     /locus_tag="VKQ27_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY88453.1"
                     /translation="MSTLPQRTGPLVGLKVLEIGHYIAAPFCTRILADLGAEVIKVEP
                     PGGDPFRGWGAAVDGHSVWFSVHGRNKLSVVLDLKRDWATFLKLTARADVLVENLRAG
                     GLERLRLGPDVLHGVNPRLVIARISGYGQDGPYRDKPAFGAIGEAMGGIRHLTAHPAG
                     SSDLPPPRCGISISDDLAGLYAAIGLLSALWQRDVTGTGIGRVIDVNLVDSVFSLMEG
                     MLPEYAMDGRVRQPVGAAIETAAPTNTYPCADGKWLCIAGNSDLIFARLMEAIGRAEL
                     ASDPLYATNGLRCANRAALDAAIADWTRTLPAKEAEERLEVADVPCSRLFDIADCAND
                     PHFRARGSVLEVADKLIGRTLHPGPVIRMDGEAPEAVVAWPGPAAGEHTEYVLKELLR
                     E"
     gene            2782..2964
                     /locus_tag="VKQ27_05670"
     CDS             2782..2964
                     /locus_tag="VKQ27_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88454.1"
                     /translation="MDKELIAKLAQAAGLTKALAEFPADVTMAAEQALGNQGAIAYPS
                     DPAAEPWPPMRAGNGV"
     gene            2961..>3724
                     /locus_tag="VKQ27_05675"
     CDS             2961..>3724
                     /locus_tag="VKQ27_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY88455.1"
                     /translation="MNDLHWLTAVEATRAFAARKLSPVELMTAALARIERLDPQLHAF
                     IRLDADAAMDAARAAEAEITKGRVRGPLHGVPVGIKDIIDVAGLPTTCHSKILVGNVA
                     KADSVVVAKLRQAGAIVVGKLSTHEFAIGGPSFDLPFPPARNPWNPDHHPGGSSSGSG
                     AGVSAGLFPLALGTDTGGSVRNPASCCGIVGLKPTYGLVSRRGVFPLSFTLDHVGPMT
                     RTVADNALLLDAIAGHDPADPGSAPSATRYFGRMLDR"
BASE COUNT          661 a         1324 c         1110 g          629 t
ORIGIN      
        1 agttcttcag gtccaccagg aactgcgtcg caccggcgtg gcgatcgacc tcttcctgcc
       61 cgtcgctcgc catcttgcag aacaggatca tgtagtcggc gcgatgcgcg ttcgaggtcc
      121 aaagcttgct gccattgatg cggaagccgc catcgacctt gaccgcacgc gttttggtac
      181 cggcaaggtc ggagccggaa tccggctcgc tcatgccgat gcagaacgac agttcggcgc
      241 ggacgatgcg gggaaggaag tagtccttct gctcgtcggt gccataacgc aggatgttcg
      301 ggccgctctg gcgatcggcg gtccagtgga agccgacggg cgcgccggcc gcgagcattt
      361 cctcgatcat cacgtagcgc tcgaaggcag tgcgttcgtg gccgccgtac agcttcggcc
      421 aggtcatgcc gatccagccg cgctccgcca tcctgcggct gaattgctgg tcatagtcgc
      481 ggcggttgcc gagtgaggcg ctgttctcct ccagaaatgc gcgcacttcc ttgcggaagg
      541 ctttggctgc cggcggtagc tcgacaggat cgaagcggaa agcctggaac gtcatcctcg
      601 cgaccctctt cgttatgacc ggcctcgcag cctccctgca cgaccggtca ccacattggg
      661 cccagcatca ttcattctcc gcaagcctga tcgcgcgcgg cgttgccgcg atgagctcgt
      721 catgcgagga ggaccggtcg gatcggatga acccaaaagc cgaatattcg ctgacaccca
      781 tctgtgccgt gggcggccac cagatccgca ccagcgacca gtcattggcg gccgagacat
      841 cgatcaccgg ctggtcctcg ctcacgcggt ggtgcaccca gttcgcctgg gtcaccagga
      901 tctcgcgctg gccaagcacc tgcgacacca cggcgacatg cccgctcggc agccggccgg
      961 agcgacgcaa taccagaaca ctgccgacgg cgggcgcctg actgcgtaag tactggccat
     1021 cggcctcgta ccaccagtca gccgcatccc ccgaaaggcg cacgccgctc agggcacggg
     1081 cgaatggcgc gcactccagc gcaacagtac cgccgacgta gttgccgtcc ggaccactgg
     1141 acccgcacgc tgccaaggca aggaggagca aggtcggaca aacgcggaag agcattatcc
     1201 caagtcccag aatgacttga ggtgattata tcaacgaatt tgtccgaatc gtacaacaac
     1261 tgcctggaat ccgctttgca gtcatatgcc cggtcctgcc tgctactccc gcagcaactc
     1321 cttcagcaca tactcggtat gctctcctgc cgccggtccc ggccaggcca ccacagcctc
     1381 gggcgcctca ccatccatcc tgatcacggg tcccggatgc agcgtccgcc cgatcagctt
     1441 atcggccact tccagcaccg agccgcgcgc gcggaaatgc ggatcattgg cgcaatcggc
     1501 gatatcgaac agccgcgagc acggcacatc cgccacctcc agccgttcct ccgcctcctt
     1561 cgccggcagt gtccgcgtcc agtcggcaat tgcagcatcc agcgccgccc ggttggcgca
     1621 cctcaaccca ttggtcgcgt acaacggatc actcgccagc tcggcccgcc cgatagcctc
     1681 catcagccgc gcgaagatca ggtctgagtt cccggcgatg catagccact tcccgtcagc
     1741 acagggatac gtattggtag gcgctgccgt ctcgatcgcc gcccccaccg gctgccggac
     1801 ccggccgtcc atcgcgtatt ccggcagcat gccttccatc aggctgaaca cgctgtccac
     1861 aaggttgacg tcgattacgc gcccaatccc ggtgcccgtg acatcccgct gccacagcgc
     1921 cgaaagcagc ccaattgcgg catacagtcc cgccagatcg tcgctgatcg aaatcccgca
     1981 ccgaggcggc ggcagatccg agcttcccgc cggatgcgcg gtcaaatgcc ggatgccacc
     2041 catcgcctcg ccgatcgcgc caaacgccgg cttgtcccga tagggcccgt cctgcccgta
     2101 tcctgagatc cgagcgatca ccagccgcgg attaacgcca tgcagcacat ccggccccag
     2161 cctcaaccgc tccaaaccac ccgcccgcag gttctccacg agcacgtcgg cccgcgccgt
     2221 cagtttcaga aaagtggccc agtcccgctt caggtccagt accaccgaca gcttgttgcg
     2281 cccatgcacg ctgaaccaca ccgaatgccc atccacggcg gccccccacc cgcggaatgg
     2341 atcgccaccc ggcggctcta ccttgatcac ctcggcgccg agatcggcca gaatgcgcgt
     2401 acagaacggg gccgcgatgt aatggccgat ttccagcacc ttcaggccga cgagcggacc
     2461 ggtgcgttgc ggcaaggtgg acattatggg ctcctgcttc gtgaatcgtg atcatcgaac
     2521 agactgaatc cgcaaaaaca acacgtcctc gttacctttt cataagcttt tagcacccat
     2581 actcctaaca cggagccggc tgttgccggc gtcctgtttc ctccccgatg tcgcgcccta
     2641 gcggcgcggc ccccttgggc ggtgcggtcc gaagccgcgc cgcccctctt ttgcctgctt
     2701 ccgttaagcc gcccctcttt tgcctgcttc cgttaagctg tccctgatcg catgatcgcg
     2761 ggcagacgaa tggaggcttg gatggacaag gagctgatcg cgaagctggc gcaggctgcc
     2821 ggcctgacca aggcactggc ggaattcccc gcggacgtga cgatggccgc ggagcaggca
     2881 ctcggcaacc agggtgccat cgcctatccc agcgacccgg ccgccgaacc ctggccgccg
     2941 atgcgcgcgg gaaacggcgt atgaatgacc tccactggct caccgccgtc gaggccacgc
     3001 gcgccttcgc agccaggaag ctctcgcccg tcgagctgat gaccgccgca ttggcgcgga
     3061 tcgaacggct ggacccacag ctgcacgcct ttatccggct ggatgccgac gccgcgatgg
     3121 acgcggcgcg cgcggccgag gcagagatca cgaaagggcg cgtccgcggt ccgctgcacg
     3181 gcgtgccggt cggcatcaag gacatcatcg acgtcgctgg cctgcccacg acgtgccact
     3241 cgaagatcct cgtgggcaat gtggccaagg ctgactcggt ggtggtcgcg aagttgcgcc
     3301 aggccggcgc aatcgtggtg ggcaagctgt cgacgcacga gtttgccatc ggcggcccaa
     3361 gcttcgacct gccgttcccg ccggcgcgca atccgtggaa cccggaccac catccaggcg
     3421 gctcgtcatc gggctccggc gcgggcgtca gcgcgggatt attcccactg gcgctgggca
     3481 cggacaccgg cggctcggtg cggaatccag cctcctgctg cggcattgtc gggctcaagc
     3541 ccacctacgg cctggtgtcg cggcgcggcg tgttcccgct gtccttcacg ctggaccacg
     3601 tcggaccgat gacgcgcacg gtggcggaca acgcgctgtt gctggacgcg attgccgggc
     3661 acgaccccgc cgatcccggc agcgcgccgt cagcgacgcg ctatttcggg cggatgctcg
     3721 atcg
//
LOCUS       DATGEM010000179         8107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_862006, whole genome shotgun sequence.
ACCESSION   DATGEM010000179 DATGEM010000000
VERSION     DATGEM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862006"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VKQ27_05680"
     CDS             <1..376
                     /locus_tag="VKQ27_05680"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HLY88456.1"
                     /translation="CIRALCDHPRNMDDEQLDALRDVGGVIQITAVAAFLKKDAKPEA
                     VTVADFADHVDYAVRRIGLQHVGISSDFDGGGGFMGWRDASESANITVELLRRGYDRQ
                     EIAALWGGNFLRVLRIAEERAE"
     gene            376..1212
                     /locus_tag="VKQ27_05685"
     CDS             376..1212
                     /locus_tag="VKQ27_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY88457.1"
                     /translation="MRTVTLPDGTQVPALGQGTWHMGERGNAAKAEVAALKLGIELGM
                     TLIDTAEMYGNGGAEEVVAEASTGHRDKLFIVSKVYPHNASRDGVPAACERSLKRLRT
                     DRIDLYLLHWQGSHPLSDTVEAFEKLRAAGKIRYWGVSNFDTRDMQGLVRLPHGADCA
                     TNQVLYHIGSRGIEFELLPWCVEHKMPVMAYSPVGQGGSLLRSKALAAVAKRHDATPA
                     QIAIAWTMRHANVISIPKASEPSHVRQNAAAGGISLTEADLAEIDAAHPPPSRKQSLD
                     IS"
     gene            1234..2937
                     /locus_tag="VKQ27_05690"
     CDS             1234..2937
                     /locus_tag="VKQ27_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88458.1"
                     /translation="MRVAPHAGVAVVLILLAILVAALVVLAAVRSLAPAHVVRFGTMG
                     GAAAIAVLACVALVVPDMASFPLPPLWLALDPIGASFLLLAGVAATARADAGPLALAG
                     MALALIAGNGFALTAGFVAVIAPREDRRNIWPTVSIICLIVAFALMGHAGAFNAIRAL
                     QPPDAPRAAVILALILIAAIGLLRVAPLLAAYLALRALFDLCGPVQPLWWALPLLIAG
                     AAIAVVTTWHAATADTLHTTLSANVRNQFGIALVALGVALLARAVDLPSLTTLALEAF
                     WLAVTCLSLCEPLLAHAADSIERAAGTRQLDRLGGLIRGLPSTSACCLAGLFTLSIVP
                     PGLGFAAFWQTFMSLLAVSRADGLGLQLLVALTAAAIALSLTLATLSGVRLFATAFLG
                     RPRTPRAAVAEEPPRQTRLELAGLAALVVLLGIIPGLARLPAIGWSGGTAFPLDLRPA
                     YSAIVIAGLLAIAASIATWIRRQGGAREQRSEPAWMDGFSAPPPWLPFGDPATQIGPA
                     SFIDPLRRLLPSLPMPPIRTWFPRSGLLLSSLSDAVSANAIPVTLIVIVATIAAWLVA
                     L"
     gene            2934..3842
                     /locus_tag="VKQ27_05695"
     CDS             2934..3842
                     /locus_tag="VKQ27_05695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HLY88459.1"
                     /translation="MTVLAGLMAQCLHIAMMMAAAPTLAGIIGALQARLTGRTGPPIL
                     QPWRELTRLHRKHSVVAESASRITEFAPSACLAALATATWLVPSFARGMILAPFADVV
                     LIAGLLALARCAQALLATDAGTAMGGIAASRTMLLGCGTDMALLLAAFAFGLAAGTTN
                     VDQIVGLQFDSTSNLLPAAAAIALAGLVDAGVVREEPLSLELSGTDLAFIEAAGMLRL
                     LLWFDLIGAALVPFGMATADAGPVAWLLGLASWLVRTALFALGVAILRATQERLRLKC
                     ATGMLGIAMLLGVLAIVLLFSTMRTA"
     gene            3839..4339
                     /locus_tag="VKQ27_05700"
     CDS             3839..4339
                     /locus_tag="VKQ27_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88460.1"
                     /translation="MSTAFPVLALLLSIALVSARRTRMALWICAAQGACAAGTLAADG
                     SLVAPVALVLNAAALPLALRRVLEDRLPDPSPPRLWLLAVGMLLVAAVGFGRFGSGIA
                     SGVTVVLLGLLLAAQSAAPIGLLSAQNGVVLVAAAIPDLPMESTLSASVPLVPALLLA
                     HAWLRQ"
     gene            4336..5424
                     /locus_tag="VKQ27_05705"
     CDS             4336..5424
                     /locus_tag="VKQ27_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HLY88461.1"
                     /translation="MTDALPFAVVALPFIASAALAAIPSGRIGTLVNIASAAPLFVLS
                     CALPWDLPDHLVRLTTFIAMTTSWAALHEAHTPPRLYYCGFQALVGAIILALLAVDPI
                     LTWIALVIALGAVTTIVDTATSAGRPLLLCGTGLMLALLGSLLLEQAPAPAALFLLLG
                     YGGVAGLAPLHAWLVDAAAEAPAPRALLISVLLPNAPLLTFMHLGTEAAPPMLVALGL
                     LSVLIGGASLLTRPDPRRAIALSGVAMLGFVECGIGLGVDATPRLIAMLALARAALLQ
                     CRDNGPTHAAAVLTLTLLPLFALYILAEPAAAWSGWLLLPLAIGALLTSWTLPRLMPN
                     ANAVHQPPLLWAPVWLQLALVAALVVAP"
     gene            5421..6539
                     /locus_tag="VKQ27_05710"
     CDS             5421..6539
                     /locus_tag="VKQ27_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HLY88462.1"
                     /translation="MIEPCAPWPRHALPHEDWARIAEPGRSLLALWADTVQVYALLRD
                     ATGEILFASTGVIEGGFPALSPAWPLASRFERMIRDLWGHAAMGGIDTRPSLDHGRWP
                     RTAPMALRPGPPGAAEPPEFLPADDDTLDQVPIGPVRPGISPAAHLRLTVRGETIVRA
                     ETHLGYTHKGTLALIRGKSPRAAARFAARLVGEATVGHSIAFARATEAALGVEIPQRA
                     GALRDLMAHIEHMAARLDQDAATEEALRRAANTAFGHRLMMDCVVPGGVAANIAPGGL
                     NALRRALNEPLPELPEGPVSVALPNESGEGIGHAVGPHGDIWHWLRLDHGQIASAFLC
                     DPAWAHWSQLEGALIASGLDDVAETCARFDLPTSGVDL"
     gene            6536..7063
                     /locus_tag="VKQ27_05715"
     CDS             6536..7063
                     /locus_tag="VKQ27_05715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HLY88463.1"
                     /translation="MSWRPTLRWPRDAERPVDTETATSLATRLEAAAQMRLGRSLAIR
                     HLDAGSCGGCEIELRALNRAVFDLQRYGLRFVDSPRHADVLLVTGPLTRNLTEALEQA
                     YTAMPEPKFVVAVGDCAVDGGVFKGSYAVLGGVGTTLPVDLLISGCPPAPERILAGLR
                     ALLEANARSRVVSSR"
     gene            complement(7050..7277)
                     /locus_tag="VKQ27_05720"
     CDS             complement(7050..7277)
                     /locus_tag="VKQ27_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88464.1"
                     /translation="MTPSRRHQFAVPGLVLALAAFAATPAMAASSTPIHHKSSHHVAH
                     ASHHVKPHHSSSVHNVAHHSTHHSTHKVTAS"
     gene            complement(7402..7824)
                     /locus_tag="VKQ27_05725"
     CDS             complement(7402..7824)
                     /locus_tag="VKQ27_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HLY88465.1"
                     /translation="MLHLTKLAVGVRDIDHLRELQAERAQTNPPLRHRTRSFPRRHQE
                     VIDGGSMYWVISGNMLVRQRIVDIIEDQRDNGEACTGLLLDPKLVPLAGRPTKPFQGW
                     RYLRPEEAPQDLAVAPRAKGEDALPPALRRELRVLCLL"
BASE COUNT         1187 a         2778 c         2637 g         1505 t
ORIGIN      
        1 ctgcatccgc gcgctctgcg atcatccgcg caacatggac gatgagcagt tggatgcgct
       61 gcgcgacgtg ggcggcgtca tccagatcac tgccgtcgct gccttcctga agaaggacgc
      121 gaagcccgag gccgtgacgg tggccgactt cgccgatcac gttgattatg cggtgcgacg
      181 catcggcctg cagcatgtcg gcatctcgtc tgactttgac ggcggcggcg gcttcatggg
      241 ctggcgcgat gcgtcagaga gcgcgaacat caccgtggaa ctgctccgcc gtggctatga
      301 ccggcaggag atcgccgcgc tgtggggcgg caatttcctc cgcgtgctgc ggatcgccga
      361 ggaaagggcg gaataatgcg cacagtcacg ttgccggacg ggacacaggt gcctgcgctg
      421 ggacagggca cctggcacat gggcgagcgc ggcaacgccg ccaaggccga agtcgcagcg
      481 ttgaaactcg gtatcgagct cggcatgacc ctgatcgaca ccgccgagat gtatggcaac
      541 ggcggcgccg aggaagtcgt cgcggaagcc tccaccggcc accgcgacaa gcttttcatc
      601 gtcagcaagg tgtatccaca caacgcgtcc cgcgacggcg tgccggccgc ctgcgagcgc
      661 agcctcaaac gcctgcgcac cgaccggatc gatctctacc tgctgcattg gcagggcagc
      721 catcccctgt cggacaccgt ggaagccttc gagaaactgc gcgctgccgg caagatccgc
      781 tattggggcg tgtccaactt cgacacccgc gacatgcagg gcctggtccg cctgccgcac
      841 ggagccgact gcgctaccaa ccaggtgctg taccacatcg gcagccgcgg cattgaattc
      901 gagctcctgc cctggtgcgt cgaacacaag atgccggtga tggcctactc gcccgtcggc
      961 caaggcggct cgctgctgcg ctccaaggcg ctcgcggcag tcgcgaagcg tcacgatgcg
     1021 acgccggcgc aaatcgccat cgcctggacc atgcggcacg ccaatgtgat ctcgatcccg
     1081 aaggcgagcg agcccagcca cgtgcgccag aatgccgcgg caggcggcat ttcccttacg
     1141 gaagccgacc tcgccgagat cgatgccgcg cacccgccac cgtcgcgtaa gcaatcgctc
     1201 gacatttcct agggctgcta tccttccagc cgtgtgcgag tcgcgcccca cgccggtgtc
     1261 gccgtggtcc tgatcctgct cgcgattctc gtcgcggcgc tggtggtgct ggcggctgtc
     1321 agatcgctgg cgccggcgca tgtggtgagg tttggcacaa tgggcggtgc cgcggcgatc
     1381 gcggttctgg cgtgcgtggc gctggttgtc cccgacatgg cgtcgttccc acttccgccg
     1441 ctctggctgg cgctcgatcc gataggggca agtttcctgt tgctggccgg cgtcgcggcc
     1501 acggcacgcg ccgatgccgg ccctttggcg ttggcaggaa tggcgctcgc tttgattgct
     1561 ggcaacggct tcgcgctgac agcgggcttt gtggcggtaa tcgcgcctcg cgaggatcgg
     1621 agaaatatct ggccaacagt ttcgataatc tgcctcatcg tcgcattcgc gctcatgggc
     1681 cacgctggtg ccttcaatgc aattcgggcg ctgcaaccac ccgacgcgcc gcgcgcggcg
     1741 gtcatcctgg ctctgatctt gatcgccgct atcggcctgt tacgcgtcgc tccgctgttg
     1801 gccgcctacc tcgcactgcg cgcactgttc gatctctgcg ggccggtaca gccgctgtgg
     1861 tgggcgttgc cgctgctgat cgccggagcc gccatcgccg tcgtcaccac atggcacgcg
     1921 gccacggcgg acacactcca caccaccttg tccgctaatg tccgaaacca gttcggcatc
     1981 gcactcgtcg cacttggcgt cgcgttgctg gcgcgtgcgg ttgacctccc ctcgctgacc
     2041 accctcgccc tggaggcgtt ctggctcgcc gttacgtgcc tcagcctgtg cgagccgctg
     2101 ctggcgcacg cagccgacag catcgagaga gcggctggca cccgccaact cgacaggctg
     2161 ggcggtttga tccgaggcct gccatcaacc tccgcctgct gcctcgccgg cttgttcacg
     2221 ctctccatcg tcccacccgg actcggcttc gcggccttct ggcaaacctt catgtcgctg
     2281 ctggctgtca gcagagcaga cggcctcggc ttgcaactgc tcgtcgcgct gaccgccgca
     2341 gccatcgcgc tatcgctgac actcgcgacg ctgtccggcg tccggctgtt cgccacggct
     2401 ttcctcggcc gcccgcgcac gccccgcgcc gccgtagccg aagagccccc ccgtcagacg
     2461 cgcctcgagc ttgctgggtt ggcagcgctc gtcgtactgc tcggcatcat ccccggtctg
     2521 gcgcgccttc ctgccatcgg ctggtctggc ggtacggcat ttccactcga tctgcgcccg
     2581 gcctactccg ccatcgtcat agcaggactg ctggcgatcg ctgccagcat cgccacctgg
     2641 atccggcgtc agggcggagc acgcgagcag cggagcgaac cggcgtggat ggacgggttc
     2701 tcggcccccc cgccctggct gccattcggc gaccccgcaa cgcagatcgg tcctgcatcc
     2761 ttcatcgatc cgctccggcg cctgttgcca tccttgccga tgccgcccat tcgcacatgg
     2821 tttccccgct ccgggttgct gttgtcgagt ctctcggatg ccgtcagtgc caatgcgatc
     2881 ccggtcacat tgatcgtcat cgttgccacc attgccgcct ggctggtcgc attgtgactg
     2941 tgttggccgg cctcatggcg caatgcctgc acatcgcgat gatgatggca gcagcgccga
     3001 cgctcgcagg catcatcggg gcgttgcaag ctcgcctgac tggccggacc ggcccaccga
     3061 tccttcagcc atggcgcgag ctgacacgcc tgcatcggaa acattccgtc gtggcggaaa
     3121 gtgcctcccg catcaccgag ttcgcgccat cagcctgcct ggctgcattg gcgaccgcga
     3181 cgtggctggt gccgtccttc gcgcgtggca tgatcctcgc gccgtttgcc gatgtggtgc
     3241 tgatcgctgg cctgctggcg ttggctcgtt gcgcacaggc gctgctggcg acggatgcgg
     3301 gcaccgcaat gggcggcatc gccgccagcc gcacgatgtt gctgggctgc gggaccgaca
     3361 tggcactgct gctggccgcg ttcgccttcg ggctggcagc gggcaccacc aatgtcgacc
     3421 agatcgtcgg actgcagttc gacagcacta gcaacctgtt gcctgccgcg gcggccattg
     3481 cgttggctgg cctggtggac gccggtgtcg tgcgtgagga accgctgtcg ctcgaattgt
     3541 cgggcaccga cctcgcattc atcgaagccg caggcatgct gcggctgctg ctctggttcg
     3601 atctgatcgg agccgccctg gtaccgttcg gcatggcgac cgccgatgct ggcccggtgg
     3661 cctggttgct tgggctggcg tcctggctcg tacgcaccgc gttgttcgcc ctgggcgtcg
     3721 ccatcctgcg cgcaacgcag gagcgcctga ggcttaagtg cgccaccggc atgctcggga
     3781 tcgcgatgct gctgggcgta ttggcgattg tattgctgtt cagcacgatg aggacagcgt
     3841 gagcacggca ttcccggtcc tggctctgct cctgtctatc gccctcgtaa gtgctcgacg
     3901 caccaggatg gcgctgtgga tctgcgccgc ccagggggca tgcgcggcag gcacgctcgc
     3961 cgcagatggt tccctcgtcg cgccggtggc tcttgtgctg aacgctgccg cgctaccgct
     4021 ggcattgcga agggtgctgg aggaccggtt gccggacccg tcgccaccac ggctttggct
     4081 gcttgccgtt ggcatgttgc ttgtcgcggc cgttggcttc ggcaggttcg gaagcggcat
     4141 tgcgtccggt gtcactgttg tgctgctggg cttgctcctg gccgcgcaaa gtgcggcgcc
     4201 cattggcctg ctgtcggcac agaacggcgt ggttctggtc gccgccgcaa ttccggactt
     4261 gccgatggaa tcgacgctgt cagcgtccgt gccgctggtt ccggcacttc tgttggctca
     4321 cgcatggctt cgacagtgac agacgccctg ccctttgccg tcgtcgcgct gcctttcatc
     4381 gcgtccgccg cgctggcagc gatcccatcg gggcgtatcg gcaccctggt caatatcgca
     4441 tccgcggcgc cgctgttcgt gctgagctgc gcccttccat gggatctgcc agaccatctg
     4501 gtgcggctca ccaccttcat cgccatgacc acgagctggg ccgctctgca cgaggcccac
     4561 accccgccca ggctgtacta ctgcggcttt caggcgctgg tcggtgctat cattctggca
     4621 ttgctggcgg tcgatccgat cctgacatgg atcgcgctgg tgatcgcgct cggcgccgtc
     4681 acaacaattg tcgatacggc cacgtcggcg ggtcgccctc tgcttctgtg cggcacagga
     4741 ttgatgctgg cgctgctggg aagcctgttg ctcgagcagg cgccggcacc tgccgcgctg
     4801 ttcctgctgc tcggctacgg cggcgtcgcg gggctcgcgc cgctgcatgc atggctggtg
     4861 gacgcggcgg ccgaggctcc agcgcctcgc gcactcctga tcagcgtatt gttgccgaat
     4921 gcgccgctgc tcaccttcat gcatctcggc accgaggcag caccgcccat gctggtcgcg
     4981 ctgggactgc tatcggtgct gattggcgga gcgtccctgc tcacacgccc cgacccgcgc
     5041 cgagccattg ccttgagcgg tgtcgcgatg ctcggcttcg tcgagtgcgg catcggtctg
     5101 ggtgtggacg caacgccacg gctgatcgcc atgctggcac tcgcccgcgc cgcgctgctg
     5161 caatgccgcg acaacggccc gactcacgcg gccgctgtgc tgacactgac gctgctgccg
     5221 ctgttcgccc tgtatatcct ggcggaaccc gccgctgcat ggtcaggctg gttgctgctt
     5281 cccctcgcca tcggcgccct gctcacgagc tggacactgc cgcgcctgat gccgaacgcc
     5341 aacgcggtac accagccgcc actgctgtgg gcgcctgtat ggcttcagct tgctctggtc
     5401 gcggcactcg tggtggcccc atgatcgaac cctgtgcgcc atggccgcgg catgccctgc
     5461 cgcacgagga ttgggcacgc atagcggagc cgggacgttc gctgctggcg ctgtgggccg
     5521 acaccgtcca ggtctatgcc ctgctgcgcg acgcaaccgg cgagatcctg tttgcatcta
     5581 caggcgtgat cgaaggcggc tttccggcgc tgtcgccggc atggccgttg gcatcccggt
     5641 tcgagcgcat gatacgcgac ctgtggggtc acgcggcgat gggtggcatc gatacgcgcc
     5701 cctcgctgga tcacggtcga tggccgcgca ccgcacccat ggcattgcgt cctgggccgc
     5761 caggcgctgc cgaaccgcca gagtttctgc ccgccgatga cgacacgctg gaccaggtgc
     5821 caattgggcc ggtacgcccg ggcatctctc cggccgcgca tctccgcctg acagtgcgcg
     5881 gtgagaccat cgtgcgggcc gagacacatc tcggctacac gcataaaggc acactggcgc
     5941 tgatccgcgg caaatcaccc cgcgccgccg cccgctttgc cgcgcgcctg gtgggcgagg
     6001 cgacagtcgg acattccatc gcctttgccc gcgcgactga ggccgcgctg ggtgtggaga
     6061 tcccgcagcg cgccggcgcg ctgcgggacc tgatggcaca catcgaacat atggccgcca
     6121 ggctggacca ggacgccgca accgaggaag ccttgcggcg tgctgcgaac accgcgttcg
     6181 gccaccgcct gatgatggat tgtgtcgtcc ccggcggcgt cgccgccaac atcgcgcctg
     6241 gtgggctgaa cgcgctccgc cgcgccctga acgaaccgct gcccgaactg cccgaaggcc
     6301 cagtctccgt ggcacttccc aacgaaagcg gtgaaggcat cggccacgcc gtcggcccgc
     6361 acggcgacat ctggcactgg ctccggctcg accacggcca gatcgccagc gccttcctct
     6421 gcgacccggc ctgggcgcac tggtcgcaac tcgagggcgc cctgatcgcc agcggtctgg
     6481 acgacgtcgc cgaaacctgt gctcggttcg atctgccaac ctccggagtg gatctgtgag
     6541 ctggcgcccg acccttcgat ggccccgcga cgctgaacgg ccggttgaca ccgaaaccgc
     6601 cacctctctc gccaccagac tggaagccgc ggcccagatg cggctcggcc gcagcctggc
     6661 gatccggcac ctggatgccg gcagttgcgg cggctgcgag atcgagttgc gcgctctgaa
     6721 ccgtgccgtc ttcgacctgc agcgctacgg cctgcgcttc gtggacagcc cgcgccacgc
     6781 cgacgtattg ctggtcacgg gtccgctcac gcgcaatctg accgaagcgc tggaacaggc
     6841 ctatacggcc atgccggagc caaaattcgt cgtggcagtg ggggattgcg ccgtggatgg
     6901 cggcgtcttc aaaggcagtt acgcagtact gggcggcgtt ggcacaacat tgccggtcga
     6961 tcttctgatc agcggctgcc cgccggcacc ggaacgcatt ctggcaggac tgcgcgcgct
     7021 gttggaggcg aacgcgcgca gccgggtcgt cagctcgcgg tgaccttgtg cgtcgagtgg
     7081 tgcgtcgagt gatgcgccac gttgtgcacc gatgaagagt ggtgcggctt cacatggtgc
     7141 gacgcatggg caacatggtg cgaggacttg tggtggatag gtgtcgagct tgcggccatg
     7201 gccggcgtgg cggcgaatgc ggcgagcgcc agaacgaggc cgggcacggc gaactggtgt
     7261 ctgcgcgagg gtgtcactgg gaggctccaa aattacaggc gttgcggaat acggctgaga
     7321 gacgggtcga ataagggttc ttacccgact ggaatcatta tcgtatgctt ggcaagagtc
     7381 gcgcaagcgt catttcgctt cctacagcag acacagtacc cgcaattccc gccgcagtgc
     7441 tggcggcagc gcgtcctccc ccttggctcg cggcgccacc gccagatcct gtggcgcctc
     7501 ctccggccgc aggtatcgcc agccctggaa cggcttggtg ggccggccag cgagcggcac
     7561 cagcttcggg tcgagcaaca gcccggtgca cgcctcgccg ttatcgcgct gatcctcgat
     7621 gatatcgacg atacgctggc gcaccagcat gttcccgctg atcacccagt acatcgagcc
     7681 accgtcgatc acctcctgat gccggcgcgg aaacgaacgc gtgcgatggc gcaacggcgg
     7741 attggtttgc gcgcgctcgg cctggagctc gcgcagatgg tcgatatccc gcacgccaac
     7801 agcgagtttc gtgagatgca gcatcgtccg gcgactcgta tgtcagcgcc gatgatggca
     7861 gggaccgcca tgcatttgaa cagcgcgccg ccgagccgcg ataagagcgc cagagagcct
     7921 aggacattac aattgcgggc cggcagacgt gccggcgctg cgcgctaaat cctgctcgcc
     7981 accgctccag gccacgctca acgagtgcaa gccttcggct cgtcaggtgt gccacaatta
     8041 cgagggtcgt tcgcaggcga cacagcatag cgcggctgct gggcattggt cccgtaatag
     8101 ccctgcg
//
LOCUS       DATGEM010000180         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1548437, whole genome shotgun sequence.
ACCESSION   DATGEM010000180 DATGEM010000000
VERSION     DATGEM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548437"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VKQ27_05730"
     CDS             <1..1446
                     /locus_tag="VKQ27_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY88466.1"
                     /translation="AAGLQDAWLGRSPVIALTGREPPFFHHRNTYQELPHGPLYASVT
                     KFSADVTEAADLPRLLRQAWREAMTGSPRPAHLDLYGRSAAVVETSLVSEPVAVDPAL
                     QLTMPPYRPTPSDDEIERAAARLRAAKRVVIVAGAGAAASGAGPEVLALGEALAAPIG
                     TSLGGRGIIPTRHRLSIGCPGNYAAPPANQIVHEADLVVFIGCHTGDQVTHTWRIPPL
                     ATEVLQIDLDPLELGRSYPRTLGVMGDPKATLAKLLVALGKPVRDGSFADRAAGIVAA
                     WREARAPLLASNAAPILPDRLCAEITRALPEDGIVVADTGYSGIWTSTLIELNGTTQT
                     YLRAAGSLGWSFPAALGAKCAAGKRKVICWSGDGAIYYHLTELETARRRGIAVVLVIN
                     NNSGFGQGWPNIQRQQGNKPGDVRELVRFGPTNFVDVARVFGLRGIRVDEPSQIAPAL
                     REALASDETVVVDVNTDIDARAPEPWLPAGA"
     gene            1451..2164
                     /locus_tag="VKQ27_05735"
     CDS             1451..2164
                     /locus_tag="VKQ27_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY88467.1"
                     /translation="MALIFHERVGLEGRRISPFSWRIRYAFAHKGLQPTVVPTRFADV
                     DRIRSLSGQHFVPIIEHDEKVIHDSWEIACYLEDRFPTAPSLFGGAAGRGVTRLVNIW
                     SDTVLGQAMRLQIYGDFITCIDPEDRAYFRGSREALLGMTLEQCSAGRDAALPDFLAL
                     CEPLRRTLSEQPFLAGEMPAYVDYVIFSVFQWARIGSPRDVLPVGAGVDALRDWRERM
                     VALYDNLGDRFAGYPTEET"
     gene            2161..2922
                     /locus_tag="VKQ27_05740"
     CDS             2161..2922
                     /locus_tag="VKQ27_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88468.1"
                     /translation="MISYDLKGKTALVTGGASGIGFATARMMAKFGATVAVNFLVDDP
                     RGPEAVDKINAEGGKAIMAPGSVGEAGGAERMVLKAIDDLGRLDLLFNNAGTPGTRNR
                     IAPPQLDLITEELWSLLLQTNLLGVFRGSKAAAPALKASHGAIVSTASIAGLGRAGSS
                     LAYSATKAGVVSLTQNLARALAPEVRVNAIAPGAVDSSWMVEWTNEERQQSIERALLK
                     RRCQPEDLAEVVVFLGFGAAMVTGQTITVDGGLTL"
     gene            complement(2942..4315)
                     /locus_tag="VKQ27_05745"
     CDS             complement(2942..4315)
                     /locus_tag="VKQ27_05745"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLY88469.1"
                     /translation="MQNSKQTGAALWLAAAFVSATAPAALAQTAAPPIAPAAPPAAAA
                     PAPAPAPAAAADQPPPGLWVNGIHFYAQLDAGIMGNPSGPNDGLNFGRLFDDHANQVQ
                     LNQLLLTANKPLDPKDPGYQWGFKAQVMYGSDARYTQFLGELNRVDPNQRYQLDIVEA
                     NILAHLPWLTSGGIDIKAGQYPTPIGYETIDPSTNPFYSHSYIFNFALPFKHTGLLAV
                     THVNDMLDIYSDIDTGTNTTFGPLGDNNGAIGGIGGFNLTLMGGKLTVLALTHFGPDQ
                     ATRLLSGGFAAAGLPTVNANGQWRFYNDVVVTYKATDALTLVTETAFARDGFGAVGKP
                     ANAIGIAQYASYALTDTLTLNGRIEYFRDDNNFFVASYPSNNGPILAQQGFAPFVHAA
                     PGTNSTYGEMTFGVTWKPPLPAPITGLAIRPEIRWDHAFTNNHPFNAQLDNNSVTLGV
                     DAVLTVF"
     gene            complement(4406..>4960)
                     /locus_tag="VKQ27_05750"
     CDS             complement(4406..>4960)
                     /locus_tag="VKQ27_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HLY88470.1"
                     /translation="SNGRAGMAMAVTQIATAAAALAWMFAEWIAKGKPSVLGCISGAV
                     AGLVAITPASGFVLPGASIIIGVAAGVICFWCATTLKHLLGYDDSLDVFGVHCVGGII
                     GALLTGVFSYGPLSATDASPDGSPGGLAQLWIQCQGVAATLVYSGVGTLILLMITKAL
                     VGLRVNEEEEREGLDLVLHGEQVF"
BASE COUNT          866 a         1437 c         1731 g          926 t
ORIGIN      
        1 gcggcggggc tgcaggatgc gtggctaggg cgcagtccgg tgatcgcgct gaccggccgc
       61 gagcctccgt tctttcatca ccgcaacacc tatcaggagt tgccgcatgg gccgctctat
      121 gcatcggtga ccaagttctc cgccgatgtg accgaggcgg ccgatcttcc gcgcctgctg
      181 cgccaggcat ggcgtgaggc aatgaccggg tcgccacgac cggcacatct cgatctgtat
      241 ggtcgctcgg cagcggtcgt ggaaaccagc ctcgtcagcg agccagttgc tgtcgatccg
      301 gcgttgcagt tgaccatgcc gccgtatcgc ccgacgccgt cggatgatga gatcgaacgt
      361 gccgccgcca gactgcgggc agcaaagcgt gtggtgatcg tcgcgggagc gggggcagcc
      421 gcttccgggg ccggtccgga agttctggcg ctgggcgagg cgctggcggc gccgatcggt
      481 acctcactgg gtggccgagg cataattccg acacggcatc ggctgtcgat tggctgtcca
      541 gggaactatg ctgcgccgcc ggcgaaccag atcgtgcatg aggcggacct ggtggtcttc
      601 atcggctgcc acaccggtga ccaggtcacg cacacctggc gcattccgcc tctggcgacc
      661 gaggtgttgc agatcgacct cgatccgctg gagcttgggc ggtcgtatcc gcgcactctg
      721 ggcgtgatgg gcgatcccaa ggcgacattg gcgaagctgc tggtggcgct gggcaagccg
      781 gtgcgagatg gctcgttcgc tgatcgcgcc gccgggattg tcgcggcctg gcgcgaggcg
      841 cgtgcgccat tgctcgccag caatgccgcg ccgatcctgc cggacaggct gtgcgccgag
      901 atcacgcgtg cgttgcccga ggatggcatc gtggttgccg ataccgggta ttccggcatc
      961 tggacctcga cgctaatcga gctgaatggc acaacgcaga cgtatctacg cgcggccgga
     1021 tcacttggct ggtcgtttcc ggccgcactt ggcgcgaaat gtgctgcagg caaacgcaag
     1081 gtgatctgct ggtctggaga tggcgccatc tactatcacc tgaccgaact ggagacggcg
     1141 cggcggcgcg gcattgccgt ggtactggtg atcaacaaca actccggctt cgggcagggc
     1201 tggccgaaca tccagcgcca gcagggcaac aagcctggag acgtgcgcga actcgtgcgt
     1261 ttcgggccaa ccaacttcgt cgatgtggcg cgggtgttcg ggctgcgcgg gattcgcgtc
     1321 gacgagccgt cgcagatcgc accggcgctg cgcgaggcgc tggcatcgga cgaaactgtc
     1381 gtggtggatg tcaatacgga tatagatgcg cgagcaccgg aaccgtggtt gccggcggga
     1441 gcatgagggg atggcgctga tcttccacga acgcgttggt ctcgagggac ggcggatcag
     1501 cccgttctcc tggcgcatac gctacgcgtt cgctcacaag gggctgcagc cgaccgtggt
     1561 gccgacgcgc tttgctgatg tcgatcgcat ccgcagcctg agcggtcagc acttcgtgcc
     1621 gatcatcgag catgacgaga aggtgattca cgacagttgg gagatcgcct gctatctcga
     1681 ggatcgcttc cccaccgcgc cctcgctgtt cggcggagcc gcagggcgag gcgtaacacg
     1741 gctggtgaat atctggtctg acacggtgct ggggcaggcg atgcggctgc agatctatgg
     1801 cgacttcatc acttgcatcg accctgagga ccgggcgtat ttccgcggtt cgcgcgaggc
     1861 gctgcttggc atgacgctgg aacagtgttc agccggacgc gatgcggcgc tgccggactt
     1921 tctggcgctg tgcgaaccgt tgcgccgcac gttgtcggag cagcccttcc tcgccggtga
     1981 gatgccggcg tacgtggatt acgtcatctt ctcggtgttc cagtgggcgc gtatcggcag
     2041 cccgcgtgat gtgttgccgg taggcgccgg cgtggatgct ctgcgcgact ggcgggagcg
     2101 gatggtggcg ctgtatgaca atctggggga tcgatttgcc ggttatccga cggaggaaac
     2161 atgatcagct atgacctgaa ggggaagact gcgctggtga ctggcggcgc atcaggtatc
     2221 ggcttcgcga ctgcgcgaat gatggcgaag ttcggcgcca cggtggcggt caatttcctg
     2281 gtggacgatc cgcgggggcc ggaggcggtg gacaagatca atgccgaggg cggcaaggcg
     2341 atcatggcgc cgggcagtgt cggcgaggca gggggcgccg aacggatggt gctgaaggcg
     2401 attgacgacc tgggtcggct tgatctgttg ttcaacaacg ccggcacgcc tggaacacgg
     2461 aacaggatcg ctccgccgca actggacctg atcacggaag agctatggtc cctgctgctt
     2521 cagaccaatc tattgggtgt gttccgaggg tcgaaggcgg cggctccggc gctgaaggcg
     2581 tcgcatggcg cgatcgtatc gaccgcttcg atcgcggggc ttgggcgcgc gggcagcagc
     2641 ctggcgtaca gcgcgaccaa ggcaggcgtg gtgagcctga cgcagaatct ggcgcgcgcg
     2701 ctggcgccag aggtgcgggt gaatgcgatc gcgccgggtg cggtggacag ttcatggatg
     2761 gtcgaatgga ccaacgaaga gcggcagcaa tcgattgaac gtgctctgct gaagcggcgc
     2821 tgccagccgg aggatctggc cgaggtcgta gtgttccttg gcttcggtgc agcaatggtg
     2881 acgggtcaga cgatcacggt ggatggggga ctgacgctgt aggcgtcagc ccccgttccg
     2941 atcagaagac cgtcagcacc gcatcgacgc ccagagtgac ggaattgttg tccagctggg
     3001 cattgaacgg gtggttgttg gtgaaggcgt ggtcccagcg tatctctgga cgtatcgcca
     3061 gcccagtgat cggcgccggc agcggcggct tccaggtaac gccgaacgtc atctcgccgt
     3121 aggtggaatt tgttcccggt gccgcgtgca cgaacggagc gaagccctgc tgcgccagga
     3181 tggggccgtt gttgctgggg taagacgcga cgaagaagtt gttgtcatcg cggaagtact
     3241 cgatgcggcc gttcagcgtg agcgtgtcgg tcagcgcgta ggacgcatac tgcgcaatgc
     3301 cgatggcatt ggcgggcttg cccaccgcgc caaagccgtc gcgcgcgaat gcggtctccg
     3361 tgaccaaggt cagcgcgtcg gtcgccttgt acgtgactac gacgtcgttg tagaagcgcc
     3421 actgaccatt ggcgttgacc gtcggcaatc cagcggcggc gaagccaccg gacaacaggc
     3481 gggtcgcctg atctggtccg aaatgtgtca gtgccagtac ggtgagcttg ccgcccatca
     3541 gagtgaggtt gaaaccgccg atgccgccga tcgcaccgtt gttgtcacca agcggaccga
     3601 acgtcgtgtt ggtgccggta tcgatgtcgc tgtagatgtc cagcatgtcg ttgacatgcg
     3661 tcaccgccag caagccagtg tgcttgaacg gcagggcgaa gttgaaaatg taagaatgcg
     3721 agtaaaacgg attggtcgag ggatcgatcg tctcgtagcc gatcggcgtc gggtactggc
     3781 cagccttgat atcaatgcca ccggacgtga gccatggcag gtgtgcgagg atattcgcct
     3841 cgacgatatc tagctggtag cgttgattgg gatcgacgcg gttcaactcg ccgaggaact
     3901 gcgtgtaccg cgcatccgag ccgtacatga cctgagcctt gaagccccat tggtagccgg
     3961 gatctttcgg gtcgagcggc ttgttggcgg tcagcaggag ctggttgagt tggacctgat
     4021 tggcatggtc gtcgaacagg cggccgaagt tgaggccgtc gttcgggcct gatgggttgc
     4081 ccatgatacc ggcgtccaac tgcgcgtaga aatggatgcc attgacccac aggccaggtg
     4141 gtggctgatc agctgccgcg gctggtgcgg gagcaggagc cggcgcggcc gcagcgggag
     4201 gggccgctgg cgcgatcggt ggtgccgccg tctgagcaag cgcggccggt gcggtggccg
     4261 acacgaaggc tgccgccaac cagagtgcgg ctcctgtctg tttgctgttt tgcatggttg
     4321 cgattgttac cttcccgttc ccctgatttt taggcacgta aaaggggtgc cgacaatgcg
     4381 gcaccccata taaatcggcg attccctaga acacctgttc gccgtgcaac accagatcga
     4441 ggccctcgcg ttcctcttct tcgttcacac gcagtccgac cagtgccttg gtgatcatca
     4501 ataggatcag cgtcccaaca ccgctgtaga caagtgtagc tgcgacgccc tggcactgga
     4561 tccagagctg cgcgagccct cccgggctgc catccggcga agcgtccgtt gccgacagcg
     4621 gtccgtagga aaacacgcct gttagcaagg cgccgatgat gccgcccacg cagtgcacgc
     4681 caaacacgtc gaggctgtcg tcgtagccca gcaagtgctt cagcgttgtg gcgcaccaga
     4741 agcagatcac accggctgcg actccgatga tgatcgaggc gcccggcagt acgaagccgg
     4801 aagccggggt gatcgccacc aacccggcaa ccgcgccaga gatgcagccc aggacggaag
     4861 gtttgccctt ggcgatccac tcggcgaaca tccaggcgag cgcggcagcc gcggtggcga
     4921 tctgcgttac cgccatggcc atgccggcgc gaccgttcga
//
LOCUS       DATGEM010000181        12625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1223407, whole genome shotgun sequence.
ACCESSION   DATGEM010000181 DATGEM010000000
VERSION     DATGEM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223407"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VKQ27_05755"
     CDS             complement(<1..664)
                     /locus_tag="VKQ27_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLY88471.1"
                     /translation="MFNYYRKELDWGGRKLILETGKIARQADGAVMARYGDTIVLCTA
                     VGVKSARPGQDFFPLTVNYQEKAFAAGKIPGGFFKREGRPSEAEVLKSRLIDRPIRPL
                     FPDNFRNEVQVVATVLSHDMENDPDIVAMIGCSAALTLSGIPFFGPVAAARVGYEDGQ
                     YILNPTEDQLERSKLDLVVAGTSEGVLMVESEAQELSEDVMLGAVTFGHQSFQPVIQA
                     IIE"
     gene            complement(992..1330)
                     /locus_tag="VKQ27_05760"
     CDS             complement(992..1330)
                     /locus_tag="VKQ27_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY88472.1"
                     /translation="MQATPAPLSLAAALALPRTPGRSVEVFVDGDLEVRFAARPTNGP
                     QVPHLKDEIYFVAAGKGRYRVEDSVTDVAVGDVLFCAAHVRHGFEDISDDFCVWVLFY
                     GPPRDRPGTL"
     gene            complement(1330..1599)
                     /gene="rpsO"
                     /locus_tag="VKQ27_05765"
     CDS             complement(1330..1599)
                     /gene="rpsO"
                     /locus_tag="VKQ27_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLY88473.1"
                     /translation="MSITAERRTVLIGEYATGANDTGSPEVQVALLSERISNLTEHLK
                     THAKDFHSRRGLLMLVGRRRRLLDYLKAKDTKRYEALIGRLNLRR"
     gene            complement(1624..2550)
                     /gene="truB"
                     /locus_tag="VKQ27_05770"
     CDS             complement(1624..2550)
                     /gene="truB"
                     /locus_tag="VKQ27_05770"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HLY88474.1"
                     /translation="MPRRKLRGRPLDGWLIIDKPPGLTSTDVVNRVRRAFDAQKAGHG
                     GTLDPLATGVLPIAFGAATKTVPYVMDGTKLYRFTLHFGEARDTDDADGKVTATAPTR
                     PTDEQIRVVLPRFMGDIMQVPPAFSAIKVAGERAYDLARDGRPPALEPRPARVDRFEL
                     LERVNPDTAIFEVASGKGVYMRSLARDLSIACGTLGHIAALRRLRVGPFTEAQAIPLD
                     KCVATEDTAPASPDLLLPVATALADIPALALTETEASGLRHGQAINLVTLMGRIPGTA
                     EPDGGLVRAMAGARVIGLARLEGGWMKPERIL"
     gene            3076..4110
                     /locus_tag="VKQ27_05775"
     CDS             3076..4110
                     /locus_tag="VKQ27_05775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLY88475.1"
                     /translation="MPTGLWRRLFPKTEFGLCYHMVSDGHPLHVRHYPILSTKEFEND
                     LLYLRHHFNFISYDELVRRRANPQPVRDNSVILTFDDGFAECSTVVAPVLRRHGIDCV
                     FFIITDLIDNATLFRETEAALCINAVCQMPVETVAAILDEVGLASHLRPPPNPTRLVG
                     TRTPLGISGLDSSSDRRLWPLLHWLLTADGNSVPLLRRLSARLGVDPQRYLTEVQPYQ
                     SASQIRQLRAEGFTIGAHSCSHRWLQGLSKDAARHEIVESCRVVRDITGQDTVPFAFP
                     YFGGGIARTWLADIRKQHEFVGLFFDTDGLREDAPFVVQRVFGERFGHDRTVDGILRR
                     AWSRRPAWYW"
     gene            complement(4146..5735)
                     /locus_tag="VKQ27_05780"
     CDS             complement(4146..5735)
                     /locus_tag="VKQ27_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88476.1"
                     /translation="MTGWAYPLQEALDSIEAGEPARAEAICRSALEADRSNQALLLLC
                     GLAVGAQGDLERAWPLLRGVAHAREHGSDPRHEMAGLTVRQRCRQLLVALYRTCLRQL
                     PDDDQLRYCVAEFLHEAHEFDEAAQILGEGLRRHPRWPAAHQLLGIIHADRGQYGAAI
                     GQFRLVTQLVPEQAGGWANLGAMLKVEGRFDAALKAYDKAVALAPQDARIRVNRTVAL
                     LHAGRLLEAWRDFEWRLALPEHQDASAHWLMPQLAELGQVAGHTILVTHSDGFGDTLQ
                     FMRYVPLLAEHGARVIAWVPKLLERVMRSLDGVADILTGDRPVPHCDFHSPFVSLPRV
                     FATTLETIPRAVPYLPVARDLVERWGRWLPDDGLRVGLVWSGQSRPHVPGFDVLDARR
                     STTLATFAPLARVPFAHFINLQMGPAADQARDSPPGLALIDPMAEVSDFADTAGIVAN
                     LDVVVSVDTSVVHLAGAVGKPVLMLDRYDNCWRWLSGRTDSPWYPKLRIFRQKRPGEW
                     GPVVARVANALTVMAERRAAA"
     gene            complement(5809..7344)
                     /locus_tag="VKQ27_05785"
     CDS             complement(5809..7344)
                     /locus_tag="VKQ27_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HLY88477.1"
                     /translation="MINWTALIVFLLLFGLVTWLGFRAARWRRGDLTTLHEWGLGGRR
                     FGSWVTWFLIGGDLYTAYTFIAVPAAMFGVGALYFFAVPYTIMVYPIMYIVFPRLWSV
                     AHKHGYITGADFVRGRFGNRWLALVVALTGILATMPYIALQLVGIQVVIGAMGIETTG
                     LGGDLPLIIAFVILAAFTYTSGMRAPAMIAIVKDLLIYIAVFAAVVAVPMHLGGYDKV
                     FSAIPAAKLLLATPPAGSMGSFSVYATLALGSAGALFLYPHSITGVLSASSARVVRRN
                     SAALPAYSLMLGLLALVGFMAIAVGVQSMPEFADGFKHYGNNFSVPALFMALFPSWFV
                     GVAFAAIAIGALVPAAIMSIATANLFTRNIYREFIRPDCTDAQESQMAKLVSLVIKAG
                     ALIFIFAIPFQYAIWLQLLGGIWIIQTLPPVILALYTRMLNGWALLIGWAVGFGLGTW
                     MFITTNFSPVYPLHIMGITVPCYAALASVVVNVVVSFVLSLFLNAVWSDRHKDVTVAS
                     DYA"
     gene            complement(7347..7559)
                     /locus_tag="VKQ27_05790"
     CDS             complement(7347..7559)
                     /locus_tag="VKQ27_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HLY88478.1"
                     /translation="MPARHDEHQPVRPVAWARLLLLIPFVAILWVPFYNSVEPSVAGI
                     PFFYWYQLLWIVLGAVIIVIVYKIEN"
     gene            7641..8795
                     /locus_tag="VKQ27_05795"
     CDS             7641..8795
                     /locus_tag="VKQ27_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY88479.1"
                     /translation="MKITEVLAHPLSAPYEHPRWTAHERMERDQMILVEVRTDQGLTG
                     YGEVAGGPQKLICDLIRTFAGVIAGMDPLGHVEIWEKLFSLTSPRPGGIGGWDGMPAP
                     LPRIQRPQAMAALGGIDIALWDLKGKAVNLPVFRLLGGTRTEVFTYATGGYYREGEAL
                     EACAEEFGRFAASGYRAVKLKTGALSLADEVTRIRTVRGTIGADVLLMLDMNAPYDVA
                     GCIRFAEAVAPYDIFWLEEPLHWYLQPADYVRLAAASPIPLAHGEREWHRFTMRDFLD
                     SGAIRYVQFDSTRAAGFTESLRIAHYAEQKGVIIAPHSALHVHAHLVSAFGDAAFGAE
                     SIGDHGRHPIHHRIHRGGAGVRDGMATLTEAPGFGMDIDWTEVERFRADI"
     gene            8798..9136
                     /locus_tag="VKQ27_05800"
     CDS             8798..9136
                     /locus_tag="VKQ27_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88480.1"
                     /translation="MLLSLCLVLAAATIGGVLAVRWRPTFGLLHGLIGAAALVALVLT
                     LSGPPRGVAMGVGSFGRIAAWLLAAALLVGLGIAAVRLRTRQVPTFVIGVHATIAISG
                     IVILAAYTLV"
     gene            complement(9108..11018)
                     /locus_tag="VKQ27_05805"
     CDS             complement(9108..11018)
                     /locus_tag="VKQ27_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HLY88481.1"
                     /translation="MDTHRSAAVHRLCSPNRRLSVTVELDRAGDLCYRVERDQVVLIA
                     DSPLGLRLRDARDAAGDLEIAGVEISTTDECVPIVAGKARYARDHHNQLALTLAYRAG
                     RTVLDLIFRVYDDGVAFRYKVPRRADGNAIELEGEITGFHFPDDCTCWAMNLPNFTTS
                     HEGEYRRTRAAALRPDELIGTPLLCRAATAAFAIAEADLDNYAGAYFCGRSDGGLGVQ
                     VTLSPLPGDPTLAVRLPADCGLQSPWRVIMIADHPGQLIEAMLIHVLNPPSIIEDISW
                     IRPGKCAWDWWCGSVVSGVAQPGMNDATIRRFIDFAAEAGLEYMMIDAGWYVSGPDGE
                     NDPRSDITRSIPEIDLPALVDYASRRNVGLFVWIAWRPMAGQMDQALPQYQRLGLKGI
                     KVDFMDRNDQEMVEFYHRLLARVAQHRLMVDLHGAYPPTGLARTYPNLLTQEGVMGAE
                     YNKWSSRVTATHNVTLPYTRMLLGAMDYTPGGFRHASPGRFEARNTLPLVQTTRGQAL
                     AMYVVYDSPLVSLADTPDAYVGEPGLDFLRIVPTTWDETRFLLGEVGEFIVLARRSGD
                     QWFVGAMTNEAVRKLLVPLSFLPSGRFVATIYGDGRPAMRSVDRDTVLELPLACGGGT
                     AIDLRPAYMQPV"
     gene            complement(11126..12103)
                     /gene="lpxK"
                     /locus_tag="VKQ27_05810"
     CDS             complement(11126..12103)
                     /gene="lpxK"
                     /locus_tag="VKQ27_05810"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HLY88482.1"
                     /translation="MHPPGFWRRDGVIPRVLSPLSVITRALTARRVARPGWRAPVPVI
                     CCGNVTVGGAGKTTVALDIGQRLLAQGCCVHFVLRGYGGTVRGPHRVGTGDTAATVGD
                     EALLLAAVAPTWVGADRAASARAASAAGAQVLVMDDGLQNPSLQQDIGLLVIDGATGF
                     GNGRLLPAGPLREPVAAAVSRCAAAVLIGDDVRGVAAGLPIPVLRARLVPGAEVADLR
                     GRRVVAFAGIALPDKFFGMLRAGGVELADAVSFPDHHPYSAADLARLIASGGECILVT
                     TPKDAVRLPAAVRSRVQIVGVALEWEPAGALETFLRDRGQPRELDGAQG"
     gene            complement(12107..>12625)
                     /locus_tag="VKQ27_05815"
     CDS             complement(12107..>12625)
                     /locus_tag="VKQ27_05815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY88483.1"
                     /translation="EGEDEIILAAHRKLAVDHAGLLTIIAPRHPDRGAAIAGDAYRAR
                     GESPPGEGVWIADTLNELGLWYRLAPIAFVGRSLVPPGGGQNPLEPARLGCAIAVGPH
                     TGNFSEHVELLRTAGGLAVVRDAAELAGWVDALLRDPERRQAMGEAARAAVRRQEDLP
                     HRTAAALLELMA"
BASE COUNT         2452 a         4300 c         3814 g         2059 t
ORIGIN      
        1 gttcgatgat cgcctggatc accggctgga agctctggtg gccgaaggtg acggcgccga
       61 gcatcacgtc ctcggacagc tcctgcgcct cggactccac catcaacacg ccttccgacg
      121 taccggcgac caccagatca agcttggacc gctccagctg atcctcagtg ggattcagga
      181 tgtattggcc gtcctcatac cccacccgcg ctgcagccac cggcccgaag aacggaatgc
      241 cggacagcgt cagcgccgcc gagcagccga tcatcgccac gatgtccgga tcgttctcca
      301 tgtcgtggct cagcacggtg gcgacgacct gcacctcgtt gcggaaatta tcggggaaca
      361 gcggccggat cgggcggtcg atcagccggg acttcagcac ctcggcctcc gacggccggc
      421 cctcgcgctt gaagaaccca ccgggaatct tgcccgcagc gaacgccttc tcttggtagt
      481 tcaccgtcaa cgggaagaaa tcctgccccg gccgcgcgct tttcaccccg acagcggtgc
      541 aaagcacgat ggtatcccca taccgcgcca tcacggcccc atcggcctgg cgagcgattt
      601 ttccggtctc caggatcagc ttgcggccgc cccagtccag ctctttgcga tagtaattga
      661 acattcattc gtccttacag atgcgcaggc atcggcggac gggcattccg atcgcgccat
      721 tggcccaact ggggcgggag gcggcgtctc gggtcgcatg gatggcgcgg gctgcgttac
      781 ggaaagcgcg ggcgcagccg cgggccatgt ggccggaaac gccgtcgcga aacccgcacc
      841 tcgggtcgcc ggttcggcgg tctgtcggtc cgccgcgtgt ccgcggcgaa tgggagcgga
      901 agccggctcc ctcggcgtcg tcccgcacca ccatggtgcg gggtaggcat cgtcttgccc
      961 cagcaagggc atattatatc acacggactc gctacaaggt gccggggcga tccctgggcg
     1021 gcccgtagaa caagacccag acacaaaaat cgtccgagat gtcctcaaac ccatgacgca
     1081 cgtgggcggc gcagaacaac acgtccccca cagccacgtc cgtcacgcta tcctccaccc
     1141 ggtagcggcc cttgccggca gccacaaagt agatctcgtc cttgaggtgt ggcacttgcg
     1201 ggccgttggt cggcctcgcg gcaaaccgca cctccaggtc tccatcgaca aatacctcga
     1261 ccgaacggcc aggcgttcgc ggcaaggcga gcgcggcggc gagcgacagc ggcgcgggag
     1321 ttgcctgcat cagcggcgca ggttcaaccg cccaatcagc gcttcgtaac gcttggtgtc
     1381 cttcgccttc aggtagtcga gcagccggcg acgccggccg accagcatca gcagcccccg
     1441 gcgcgagtgg aagtccttcg cgtgggtctt cagatgttcg gtgaggttgc tgatgcgctc
     1501 ggacagcagg gcgacctgca cctccgggct gccggtatca ttggcaccag tggcatactc
     1561 gccgatcagc accgtacggc gctccgcggt gatcgacatc ggaattgtct ccgtcagcaa
     1621 gggttagagg atgcgctccg gcttcatcca gccgccttcc aggcgggcaa ggccgatcac
     1681 gcgcgccccc gccatggccc tcaccagccc cccatcaggt tcggcggtgc caggaatgcg
     1741 gcccatcagc gtgaccaggt tgattgcctg gccgtgcctc aggccgctgg cctctgtctc
     1801 ggtcaaggcc agcgccggga tgtcggccag cgcggtcgcg accggaagca gcaggtccgg
     1861 ggaagccggc gcggtatcct ccgttgcgac gcatttgtcc agggggatcg cctgagcctc
     1921 agtgaacggc cccacgcgca gccggcgcag cgcggcaatg tggccgagcg tgccgcaggc
     1981 gatcgaaaga tcgcgcgcca ggctgcgcat gtagacgccc ttgcccgacg cgacctcgaa
     2041 gatggcggta tcgggattga cgcgttcgag caactcgaaa cggtctaccc gggcgggacg
     2101 cggctccagc gccggcgggc ggccgtcgcg ggccaggtcg taggcgcgct caccagcgac
     2161 cttgatggcg gagaaggccg gcggcacctg catgatatcg cccatgaacc gcggcagcac
     2221 gacgcggatc tgctcatccg tcgggcgggt gggagcggtc gccgtgacct tgccgtcggc
     2281 atcatccgtg tcacgtgcct cgccgaagtg aagcgtgaat cggtacagct tggtgccatc
     2341 catcacataa gggacagtct tggtcgcagc accaaaggcg atcggcagca cgcctgtcgc
     2401 gagcggatcc agagtgccgc catggcccgc cttctgggcg tcgaatgcac ggcggacgcg
     2461 attgacgacg tccgtgctgg tcaggcccgg tggcttgtcg atgatcagcc agccgtcgag
     2521 cggacggcca cgcagcttgc ggcggggcat ggggagagtt ccagcagggc gccaggcatc
     2581 gttgccagcg ggtggaccgg cgacctacgg gcggacggta tccatgtcaa ccatctcggg
     2641 ccatgcaccg ctacgccggc tcatgccatt gagcctgagc atgagttcca cgcttccgtc
     2701 tgccatgtct ggaaccgtcg caagaatgcg ctcatgagat aagcatgccc tggaaatatt
     2761 ttgattgatc gcaatgtcgc ggtaaccaat ccgaaattcg cttctcgtag ttctagctgc
     2821 gcctggcaag aatgcgccgt gtgcggcgct ctcaactttc gtcctccaag aactcgatgc
     2881 tcgactgacc agatgaagcg acaaggcgta agccgttctc gtgcaacggc aggcaaatga
     2941 cacggtacct gattgactgc ggggatcgcg atgctaacag ggtcagacct tgaaccgcca
     3001 gcatccaaag tcccggatca gagggccatg ttgactgcac acgcgcgcag gatcgccagt
     3061 caggcacttc atcacgtgcc gaccgggctt tggcggcgcc tcttcccaaa gaccgagttc
     3121 ggtctttgct atcacatggt gtcggacggc cacccgctac atgtccgaca ctatccgatc
     3181 ctgagcacaa aggaattcga gaacgatctg ctatacttgc ggcaccactt caatttcatc
     3241 tcttacgacg aactcgtccg acgccgtgcc aaccctcaac ccgtacgcga caactcggtc
     3301 attttaacct tcgacgatgg tttcgctgaa tgctcgacgg ttgtcgcgcc tgtcctgcgt
     3361 cgccacggta tcgactgcgt tttcttcatc attacggatc tgatcgacaa cgccacactg
     3421 ttccgggaga ccgaggcagc cctctgcatc aacgctgtct gccagatgcc ggtcgagacc
     3481 gttgcggcaa ttcttgacga ggtcgggttg gcgtcgcact tgcgccctcc acccaatcca
     3541 acgcggctcg ttggcacccg taccccgctt ggcatttccg gcctggacag ctcgtcggac
     3601 cgccggctat ggccgctcct tcactggctg ctgaccgcgg acgggaacag cgtcccgctg
     3661 ttgcgccgct tgtccgcgcg cctgggcgtg gaccctcaac gctatctcac agaggttcag
     3721 ccatatcaga gcgcttcgca gatccgtcaa ctgcgtgccg aagggttcac gataggggcc
     3781 cacagttgca gccatcgctg gcttcagggc ctctcaaaag acgcggcccg acacgaaatc
     3841 gtcgagtctt gccgcgttgt tcgcgacatc accggccagg acacggtgcc attcgcgttt
     3901 ccctatttcg gcggcggaat cgcgcgcaca tggttggccg atattcgcaa gcaacatgag
     3961 ttcgtcggtc tgttcttcga cacggacggg ctgcgcgagg atgcaccatt tgtggttcaa
     4021 cgcgtgtttg gcgagcggtt cggtcacgat cgcacggtgg acggcatcct gcgtcgggcc
     4081 tggagccgtc gaccggcttg gtactggtag ctcatgcgtc cctgcggatg agcacccgcg
     4141 atccttcaag ctgccgcgcg ccgctccgcc atcaccgtca gtgcattggc gacacgagcg
     4201 acgacgggcc cccactcccc ggggcgcttc tggcggaaga tgcggagctt cggataccac
     4261 gggctgtcgg tgcggccgct gagccagcgc cagcaattgt cgtagcggtc gagcatcagc
     4321 accggcttgc cgaccgcacc ggccaggtgc accacggatg tatcgacact gaccaccacg
     4381 tcgagattgg cgacgatgcc ggccgtatca gcgaagtccg atacctcggc catcgggtcg
     4441 atcagtgcca ggcctggtgg ggaatcacgc gcctgatccg cggctggccc catctgcaga
     4501 ttgatgaaat gcgcaaacgg aaccctggcc agcggcgcga atgtcgccag agtcgtgctg
     4561 cgccgcgcat cgagcacgtc gaaacctggg acatgcggcc ggctctgccc cgaccatacc
     4621 aggccgacgc gcagtccatc atcgggcaac cacctgcccc atcgctccac cagatccctg
     4681 gcgaccggca gatacggcac tgcacgtggg atcgtctcga gcgtagtcgc gaacacccgt
     4741 ggcaggctga cgaacggcga gtggaagtca caatgcggca ccggccgatc gcccgtcagg
     4801 atgtcggcaa caccgtccaa tgaccgcatc acccgttcca acagcttcgg cacccacgcg
     4861 atcacccgcg ccccgtgctc agcgagcagt ggcacatacc gcatgaattg cagcgtatcg
     4921 ccgaacccat ccgaatgcgt tacaaggatc gtgtggccag ccacctggcc gagttccgcc
     4981 agttgcggca tcaaccaatg ggccgacgcg tcctgatgtt ccggcagcgc cagccgccac
     5041 tcgaaatcgc gccaggcttc cagcaaccgc ccggcatgca ggagtgccac cgtccggttg
     5101 accctgatac gcgcatcctg cggtgccagc gcaacggcct tgtcataggc cttcagcgcc
     5161 gcgtcgaacc gcccctccac tttcagcatc gccccgagat tggcccagcc ccctgcctgt
     5221 tccggcacaa gctgcgtcac cagccggaac tgcccaatcg cagcgccata ctgccctcgg
     5281 tcagcatgga tgatgcccag caactgatgc gccgccggcc agcgtggatg ccggcgcagc
     5341 ccctcgccca gaatctgcgc tgcttcgtcg aattcatggg cttcgtgcag gaactccgcg
     5401 acgcagtaac gcagttgatc atcgtcaggc aactgcctga ggcatgtgcg atacaacgcc
     5461 accagcagct gcctgcatcg ctgacgcaca gtgagtccgg ccatctcatg gcgcggatcg
     5521 gaaccgtgct cgcgagcatg cgccacgccc cgcagcagcg gccaggcccg ctccagatcg
     5581 ccctgggcac cgacagcaag accgcacagc agtagcaacg cctggttgct acgatccgcc
     5641 tcaagcgctg accggcagat tgcttcggca cgcgccggct cgcccgcctc gatactatcg
     5701 agcgcctcct gcaacggata ggcccatccg gtcacaccgc aactcccccg cctgcgacgg
     5761 cctgggagtt tggcgctccg gaattaaagc cgggttatcg tggcgacatc aggcgtagtc
     5821 gctggcgacc gtgacatcct tgtgcctgtc cgaccatacc gcattcagga acagtgagag
     5881 cacaaacgac acgacgacat tcaccaccac tgacgccagc gcggcatagc acggcaccgt
     5941 gatgcccatg atgtgtagcg gatagacggg actgaagttc gtcgtgatga acatccacgt
     6001 gccaagacca aaccctaccg cccagccgat cagcagtgcc cagccattca gcatgcgcgt
     6061 gtaaagcgca aggatcacgg gaggcagcgt ctggatgatc catatgccgc cgagcagttg
     6121 cagccatatc gcgtattgga atgggatggc gaagatgaat atcagcgcac ctgccttgat
     6181 caccagcgaa accagcttgg ccatctggct ctcctgcgcg tcggtgcagt caggcctgat
     6241 gaactcgcgg tagatattgc gggtgaacag gttggcagtg gctatcgaca tgatcgccgc
     6301 cggcaccagg gcaccgatcg cgatcgcggc aaacgcgact cccacgaacc aggacgggaa
     6361 cagcgccatg aacagcgccg gcacggagaa gttgttgccg tagtgcttga agccgtcggc
     6421 gaactccggc atcgactgaa cgccgacggc aatcgccatg aaacccacca gcgccagcag
     6481 ccccagcatc agcgaatagg caggcaacgc cgcggaattg cggcgcacca cccgcgcgct
     6541 tgaagcgctc agcacgccgg tgatcgagtg tggatacagg aacagcgctc cggcagaccc
     6601 gagcgccagc gtcgcataga cgctgaagct ccccatcgag ccggccggcg gcgtggccag
     6661 cagcagcttt gccgcgggga tagcactgaa caccttgtcg tagccaccca gatgcatggg
     6721 caccgccacg accgccgcaa acaccgcaat atagatcagc aggtccttga cgatggcgat
     6781 catcgccggc gcgcgcatgc ccgaggtgta ggtgaacgcg gccaggatga cgaaggcgat
     6841 gatcagcggc aggtcgccgc ccaacccggt ggtctcgatc cccatggcgc cgatgacgac
     6901 ctggatgccg acgagctgca gcgcaatata cggcatggtc gccaggatgc cggtgagtgc
     6961 aaccaccagt gccagccagc gattgccgaa gcggccacgc acaaaatcag cgccggtgat
     7021 atagccatgc ttgtgcgcca cggaccacag ccggggaaac acgatataca tgatcggata
     7081 gaccatgatc gtgtacggca cggcgaagaa atacagcgcg ccgacaccga acatcgcggc
     7141 cggcaccgcg atgaacgtgt aggccgtgta caaatcgccg ccgatcagga accacgtcac
     7201 ccagctgccg aatcgccggc cgccgagccc ccactcatgc agcgtggtca ggtcgccgcg
     7261 ccgccagcgc gccgccctga agccgagcca ggtgaccagg ccgaacagca gcaggaaaac
     7321 gatcagggcg gtccagttga tcatggtcag ttctcgattt tgtagacgat gacgatgatc
     7381 acagcgccca gaacgatcca gagaagctgg taccagtaga agaacgggat accggccacg
     7441 ctcggttcca cgctgttgta gaacggcacc caaaggatgg cgacgaaggg gatcagcagc
     7501 agcagccggg cccaggcgac cgggcgcacg ggctggtgct catcatgtct ggcgggcatc
     7561 ggatcctccc gaatgttgcg gttcgattat cgcctgccgg ggactgatgc aaccgagcgc
     7621 cgtcattgta ggattggcgc atgaagatca ccgaagtcct ggcgcacccc ttgtcggctc
     7681 cctacgagca cccccgctgg accgcccatg agcggatgga gcgcgaccag atgatcctgg
     7741 tcgaggtccg caccgaccag ggcctcaccg gctacggcga agtcgcgggc ggcccccaga
     7801 agctgatctg tgacctgatt cgaacattcg ccggggtgat cgccggcatg gacccgctcg
     7861 gccacgtcga aatctgggaa aagctcttct cgctcacttc gccaaggccg ggcggcatcg
     7921 gcggctggga tggcatgcca gcgccgctgc cgcgcatcca gcgtccccag gcgatggcgg
     7981 cactcggcgg catcgacatc gcgctatggg acctgaaagg caaggcggtg aacctgccgg
     8041 tgttccgcct gctcggcggc acgcgcaccg aagtcttcac ctatgccacc ggcggctact
     8101 accgcgaggg cgaagcgctg gaagcctgcg ccgaggaatt cggccgcttt gccgcaagcg
     8161 gctatcgcgc ggtgaagctg aaaaccggcg ccctgtccct ggccgacgag gtgacccgca
     8221 tccgcaccgt gcgtggtacg attggcgccg atgtgttgct gatgctcgac atgaatgcgc
     8281 cctacgacgt cgctggctgc atccgcttcg ccgaagccgt cgcgccctac gacatcttct
     8341 ggctggagga accgctgcac tggtatctgc aaccggccga ttatgtccgg cttgccgccg
     8401 catcgccgat accgctcgcg catggcgaac gggaatggca ccgcttcacc atgcgcgact
     8461 tcctcgacag cggcgcgatc cgctacgtgc agttcgactc aacccgtgcg gccggcttca
     8521 ccgaatcgct ccgcatcgct cactacgccg agcagaaggg cgtgatcatc gccccccact
     8581 cggcacttca tgtccacgcg catctcgtgt cggcattcgg tgacgcagcc ttcggcgcgg
     8641 aatccatcgg cgaccacggc cggcatccca tccaccacag gatccatcgc ggcggcgcag
     8701 gggtacgcga cggcatggcg acgctgaccg aggcccccgg tttcggcatg gacatcgact
     8761 ggacagaagt cgagcgcttc agggccgaca tttagtgttg ctgctgtcgc tgtgcctggt
     8821 gctggcggcc gcaacaatcg gcggtgtgct tgccgtccga tggcgaccaa cattcggcct
     8881 gctgcacggc ttgatcggag cggcggccct cgtcgcactg gtcctgacgt tgagtggacc
     8941 gccgcgcggc gtggcgatgg gtgtcggctc attcggccgg atcgccgcgt ggctgcttgc
     9001 agcggcactg ctcgtgggat tgggcatcgc cgctgttcgc ttgcgcacgc gccaggtgcc
     9061 aaccttcgtg atcggtgtcc acgcgaccat cgcgatcagc ggcatcgtca tactggctgc
     9121 atatacgctg gtctgagatc gatcgccgtg ccaccaccgc aagccagagg cagctccagg
     9181 accgtatccc gatcgacgct acgcatggcg ggtctgccat cgccatagat cgtcgctacg
     9241 aaacgacccg agggcaggaa gctcaggggc accagaagct ttcgcaccgc ctcattggtc
     9301 atcgcgccaa cgaaccactg atcgccgctg cgacgcgcga gaacgatgaa ctcgccaacc
     9361 tcaccgagaa ggaagcgggt ttcatcccac gtagtcggca cgatgcgcag aaaatccagg
     9421 cctggctcgc ccacatacgc atccggcgtg tcagcaagcg acacaagcgg actatcataa
     9481 accacataca tcgcgagcgc ctggccacgc gtcgtctgca ccaatggcag cgtattgcgc
     9541 gcctcgaagc gccccggcga ggcgtggcgg aacccccccg gcgtgtagtc catcgctccc
     9601 agcagcatgc gcgtataagg caacgtgacg ttgtgggtag cggtcacgcg gctgctccac
     9661 ttgttgtact ccgcgcccat cacgccttcc tgcgtcaaca ggttcggata ggtacgcgcg
     9721 agcccggttg gcggatacgc gccgtgcaga tcgaccatca accggtgctg agcaaccctg
     9781 gcaagcaggc gatggtaaaa ttccaccatc tcctggtcgt tgcgatccat gaaatcgacc
     9841 ttgatcccct tcagcccgag ccgctgatac tgcggcagcg cctgatccat ctgacccgcc
     9901 atcggccgcc acgcaatcca gacgaacagg ccaacgtttc gtcgactggc gtaatcaacc
     9961 agcgccggca gatcgatctc aggaatactg cgcgtgatat ccgagcgcgg atcgttctcg
    10021 ccgtccggcc cagaaacata ccagccggca tcgatcatca tgtactcaag gcccgcttcg
    10081 gcggcgaagt cgatgaagcg ccgtatggtg gcatcgttca ttccgggctg tgcaaccccg
    10141 gaaaccacgc tgccgcacca ccaatcccag gcgcatttgc cgggcctgat ccagcttatg
    10201 tcctcgatga tcgatggcgg gttcagcaca tggatcagca tcgcttcgat gagctggcct
    10261 ggatggtcgg caatcatgat gacgcgccaa ggtgattgca aaccgcagtc agccggcagg
    10321 cgcaccgcca gagtcggatc gccaggcagc ggtgacagcg tgacctgtac acccaagcca
    10381 ccatcgctcc ggccacagaa ataggcgcca gcatagttgt cgagatcagc ctccgcgatc
    10441 gcaaaggcgg cggtcgcagc acggcagagc agcggcgtgc caatcaactc gtccggacgc
    10501 agcgcggcag cacgagtgcg gcgatattcg ccttcgtgac tcgtggtaaa attcggcagg
    10561 ttcatagccc agcacgtgca gtcgtccgga aaatgaaagc cggtgatttc accttccaac
    10621 tcgatagcgt tgccatcagc acgacgcggc accttgtagc gaaacgcaac accgtcgtcg
    10681 tagacgcgaa aaatcaaatc aagcacagtg cgccccgccc gataagccag ggtcagcgcc
    10741 agttgattgt ggtggtcgcg ggcatatcgc gccttgccgg caacgatagg gacgcactcg
    10801 tcggtcgtgc tgatttcgac gccagcgatc tccagatcgc cggcagcatc gcgcgcatct
    10861 cgaagcctaa gccccagagg cgagtccgcg atcagaacga cctggtcgcg ctcgacgcgg
    10921 tagcagagat cccccgcgcg atccaattca accgtgacgc tcaatcgccg attgggcgaa
    10981 cacaggcgat gaacggcagc cgaccggtga gtgtccatct caggcgggta tcacgaatca
    11041 cccggattgg ctcgccagca ccaaacgcat ttctgtgaat tccgtgcaat ggcttgcgcg
    11101 gcgagccaat ccgcgcaatc cgtgtttatc cttgcgcgcc gtccaattcc ctcggctgcc
    11161 cgcggtcgcg caaaaatgtc tcgagcgcgc cggctggttc ccactccagc gcaacaccca
    11221 caatctgaac cctcgaacgc actgccgccg gaagtcgcac agcatccttc ggcgtggtga
    11281 ccagaatgca ttcaccaccc gatgcgatga gccgcgcgag atcagcggcg ctgtaggggt
    11341 ggtgatcagg aaacgacacc gcatccgcca actcgacgcc gccagccctc agcatgccaa
    11401 agaacttgtc cggcaatgca atgccggcaa aggcgacaac acgccggccg cgcaggtcag
    11461 ccacctccgc ccccggcaca agccgcgcgc gcaacaccgg gattggcaga ccagccgcaa
    11521 ccccacgcac gtcatctcca atcagcacag cggccgcaca gcgagaaacc gccgcggcaa
    11581 ctggttcccg caacggcccc gccggcagca ggcgaccatt gccaaaaccg gtcgccccat
    11641 cgatcaccag caagccaata tcctgctgca acgaggggtt ctgcagcccg tcatccatca
    11701 ccagcacctg agccccggcc gcactcgccg cacgcgcgct tgcggctcga tcggcaccaa
    11761 cccacgtcgg cgcgaccgcg gccagcaaca gcgcctcatc gccaaccgtc gccgccgtat
    11821 cgcccgtgcc gacacgatgc ggaccgcgca cggtgccgcc atagccgcgc agcacgaagt
    11881 gcacgcagca accctgcgca agcaggcgct gaccaatgtc gagtgccacc gtggtcttgc
    11941 ccgcgccgcc gacggtaacg ttgccacaac agataaccgg caccggcgcg cgccagccag
    12001 gccgcgccac gcggcgcgcc gtcagtgccc ttgtgatcac tgaaagtggt gaaagcaccc
    12061 gtggaatcac gccgtcgcgg cgccagaagc ccggcgggtg cagcatctac gccatcagct
    12121 ccagcagggc agcagcagtg cggtgcggca aatcctcctg ccgccgcacc gccgcgcgcg
    12181 ccgcctcgcc catcgcctgg cgccgctcag gatcgcgcaa tagtgcatcc acccagccag
    12241 ccagctccgc agcgtcccgc acgacggcca aaccgccggc ggtacgcagc aactccacat
    12301 gctcggaaaa attcccggtg tgcggaccga ctgcgatcgc gcaaccaagg cgtgccggct
    12361 cgagtggatt ttgcccgccg cccggtggca ccaggctgcg acccacgaag gcgataggcg
    12421 ccagccgata ccagagccca agctcgttca gcgtgtcggc aatccacacc ccttcgccag
    12481 gaggcgactc accgcgcgcg cgataggcgt cgccagcgat cgcggcaccc cgatcgggat
    12541 gccgcggcgc gataatggtc aacagtccgg catgatccac cgccagcttc cgatgggcag
    12601 cgagaatgat ctcgtcctcg ccctc
//
LOCUS       DATGEM010000182         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_292763, whole genome shotgun sequence.
ACCESSION   DATGEM010000182 DATGEM010000000
VERSION     DATGEM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292763"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(780..980)
                     /locus_tag="VKQ27_05820"
     CDS             complement(780..980)
                     /locus_tag="VKQ27_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88484.1"
                     /translation="MAEVVDWGNLTAEAHGLGCYISHWTAAGDLARVALGIAVMSRFV
                     ALSNRLLWRPLANLAERHWRLG"
     gene            complement(955..1245)
                     /locus_tag="VKQ27_05825"
     CDS             complement(955..1245)
                     /locus_tag="VKQ27_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88485.1"
                     /translation="MGRVLAAMTVIDQLCDGTLPVGALGSAMATTERLPPSSTPRFAS
                     FAAIGAASLGVIFGALDAASLLLIAFSAALGALHRRRRRLERLDRGRGGRLG"
     gene            1244..1531
                     /locus_tag="VKQ27_05830"
     CDS             1244..1531
                     /locus_tag="VKQ27_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88486.1"
                     /translation="MDAERHRRHDIGERRALDMDRELAPARQHADRQAQGAAEPFRSD
                     DRSLRGLPIVKQVCRRQRDGLDRRCTLWMHRAKRQRFGSAALGISFSDRIR"
     gene            complement(1568..3244)
                     /locus_tag="VKQ27_05835"
     CDS             complement(1568..3244)
                     /locus_tag="VKQ27_05835"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HLY88487.1"
                     /translation="MNWLTDLFFGGHDTANAVAVLALVAVLGLAVGQIRVGRVKLGIA
                     GPLFVGIMLGHLGFRLDMTMLAYARDFGLLIFVYAIGITVGPGFFESFKKDGLLLNVA
                     TTAICVVGGLITALIHVGFRQPVEAAVGMFAGAVTNTPSLAAGMQMLASLGGTSDQVA
                     TPPIAYAVAYPFGILGVLIIMALLRSIFRIDIAAEAESFARARRANIRPIKTMSIEIR
                     RPEIAGMAIHDVPSVRAGGVVIAAVKHGDRVLTVGVRSVLELNDVVVAVGSQAKLEEV
                     RDSLGVEARISLQEQPGNLKVVNAVVTRTKWLGKHIFELHFLDVYGVVITRVVRSGIE
                     LVPDKALKLQFGDLLTCVGEADKLKAVTDHFGNERKALLEAQIIPIFIGLLLGVILGG
                     VPFFIPGLPVPIKLGLAGGPVIVAIILARIGSIGSLVWFMPPGTTNTVREIGITIFMA
                     AVGIYSGPSFVSTVLSARGLEWLALAAVITFIPIFAVGMVLRGVFKVNYLSLCGALAG
                     SSTSPPALVFASGIFPSEAQATAYAVVYPLTMFLRIITPQLILALFSATS"
BASE COUNT          813 a         1268 c         1253 g          644 t
ORIGIN      
        1 atcgaatcac aaaccgcctt ccgccggaat cccctacatg gacacgccaa aatatgactc
       61 agcccggaac ccgcaccatt ccgccgaaaa cccgcctcgc caaatgaaaa cttcggtcta
      121 acgctacggc ccagaccggg cgcgtacgtc tcttgggcgc actcgtagtg tgacaaacgg
      181 ccggccaagg agcgggccac ccgctgcgct gtcaacgtag cgcaatgagc tgtccccggt
      241 tccacggata gctccatccg tcaggacgat gacccaacac gtcgcaatgg ctgctgtccg
      301 agttggaacg ctccagagct gccgttccgc aatcggcccg ctcaggccag agagcggccg
      361 ggcggaagag cgcccatcgt ggtcattcca gggcgagaaa gcgcagccgg aaaccggtcg
      421 ttcgctttga cggtaggcaa agcgcgatat tgcggcacgt ttcatctcgc tgccccccgt
      481 ctcagtgagc aagcttgcat tagcttcgta tcggcttcag atttggcacg ccgtggcgcg
      541 cgtggcgcca agtgttatgt atcgccacgg aaagcgaact tgtatcgatc agcattccgc
      601 ctgacgttct acggatcgca cacgtcaaag gtgcccggcc agcaccaccg gcatcgcagc
      661 ccggatggcg aatttcgcat tgggcgcgcc cgaacgaggt catgcatggc cattccacat
      721 cgctgagtcc gacaaaacct gtgcgatcgc ggcgtttctc gcagcctaga agtgtcgcgt
      781 tagcccagcc gccagtgccg ttccgcgaga ttggcgagcg gtcgccaaag caagcggttc
      841 gacagcgcta cgaagcgcga catgacggca atgccgagcg cgacgcgcgc taaatcgccg
      901 gccgccgtcc agtgtgagat atagcagccc agcccgtgcg cctcggccgt gaggttaccc
      961 cagtcgacca cctcggccac gatcgagacg ttccagccgg cgtcggcggc ggtgcagcgc
     1021 gccgagtgcg gcgctgaacg caatcagcag caggctggcc gcatcgagcg caccgaagat
     1081 cacgcccagc gacgcggcgc cgatcgcagc gaacgaggcg aaccgcggcg tcgaggaggg
     1141 cggcagccgc tccgttgtcg ccatggccga acccaaggcc ccgaccggca gcgtgccatc
     1201 acacagctgg tcgatgaccg tcatcgcggc cagaacgcgg cccatggacg ccgagcggca
     1261 tcgccggcac gacatcggcg aacggcgtgc cctcgacatg gaccgcgagc tcgccccagc
     1321 gcggcagcac gcggacaggc aggcccaagg cgcggccgag ccgttccgta gcgacgaccg
     1381 ttcgttgcgt ggtttgccca tcgtcaagca ggtatgccgc cgccagcgcg acggtctcga
     1441 ccgccgctgc acgctctgga tgcaccgcgc caagcgccaa cggttcggca gcgcagctct
     1501 tggcatctcc ttttcagacc ggattcggtg agcgcatgcc ggaacgacgc cgtcagaccg
     1561 caagccgcta agacgttgcc gagaacaagg caaggatcag ctgcggtgtg atgatcctga
     1621 ggaacatcgt cagcggatag acgacggcat aggcggtcgc ctgagcctcc gacggaaata
     1681 tgccgctggc gaagaccagc gccggcgggc tggtggaact gccggcgagg gcaccgcaca
     1741 ggctcaggta gtttaccttg aagacgccgc gcagcaccat gccgacggcg aagatcggga
     1801 tgaaggtgat gactgcggcg agcgccaacc actccaggcc ccgggcgctg aggaccgtgg
     1861 aaacgaagct cggaccggaa tagatgccaa ccgccgccat gaagatcgtg atgccgattt
     1921 cgcgcaccgt gttggtcgtg cctggcggca tgaaccagac gagcgaaccg atgctgccaa
     1981 tgcgagcgag aataatggcg acgataaccg ggccaccggc gaggccgagc ttgatcggaa
     2041 ccggcaggcc gggaatgaag aacggaacgc caccgaggat aacgccgagc agcaagccga
     2101 tgaagatcgg gataatctga gcttccagca aggccttgcg ctcgttgccg aagtggtcgg
     2161 tcaccgcctt cagcttgtct gcctcgccga cgcaggtgag aagatcgccg aactgcagct
     2221 tcaacgcctt gtcaggcacc agttcgatgc cggagcggac gacccgggtg atgacgacgc
     2281 cgtagacatc gaggaaatgc agctcgaaga tatgcttgcc gagccatttg gtgcgcgtca
     2341 caacggcgtt gaccaccttc agattgccgg gctgttcctg caggctgatt cgcgcctcga
     2401 cccccagtga atcgcgcacc tcctcgagtt ttgcctgcga gccgacggca accaccacgt
     2461 cattcaattc cagcacggac cggacaccga cagtgagcac ccgatcgcca tgcttgacag
     2521 cggcaatgac gacgccgccg gcacgcacgg agggcacatc gtggatcgcc atgccagcga
     2581 tctccggcct ccggatttcg atgctcatgg tcttgatcgg acgaatgttg gcgcggcggg
     2641 cgcgggcgaa gctctctgct tccgccgcga tgtcgatgcg gaagatgctc cgcagcagcg
     2701 ccatgatgat gaggactccg aggatgccga acgggtaggc cacagcgtag gcgatcggcg
     2761 gcgtcgccac ttggtccgac gtcccaccga ggcttgccag catctgcatg ccagcggcga
     2821 gcgacggcgt gttggtgacg gctccggcga acattcccac cgccgcttcc acaggctgcc
     2881 ggaagccgac atggatcagt gcggtaatga ggccgccgac gacgcagatc gccgtcgtag
     2941 ccacattcag cagcaagcca tccttcttga aggattcgaa gaagcccggt ccgaccgtga
     3001 tcccgatcgc atagacaaag ataagcaggc cgaagtcgcg ggcgtatgcc agcatcgtca
     3061 tgtccagcct gaagccgagg tgaccgagca tgatgccgac gaacagaggg ccagcgatgc
     3121 ccagcttcac cctgccgacc ctgatctgac ctacggcgag gccgagaacc gccaccagag
     3181 cgaggacggc gaccgcattg gcggtatcgt gaccgccaaa gaagagatcg gttagccaat
     3241 tcatggcatt gtcctcaggt aaagatctga cgaaagtgtg cttgtcgctc gcctggcccg
     3301 tcctttgccg aagggtatgt cggccatccc ctttggctta ggggcggccc cataagcgac
     3361 gcaaagccct tgtgctaaag cttgcgtcga aaatacgaag cgacctatcc cccaagtggg
     3421 agaacgaggt tccgttcagg ggaaagtgcc gagagaccgg cgtcaagccc gttgaaattg
     3481 ttcgccgccc ttcggtcggc tctgagcaaa cgtgatcgag cacccatccc ggtcagggcg
     3541 ccatcggcgc cgccctgacc atgctctcag ccgatttttc gatagggccg gtacagcggc
     3601 tcccacactc gggcagcgat gcgcgcatcg agcgtcgccg cgtcgcatgg gtccgcgacg
     3661 ccgtcggcct gtccggtcaa gataggggtc gacgatcccg tcgcagggcg acaaatccac
     3721 ggtaagcttg ccggccatcg aagccgacga gccagtgccg acgatgtgcg cgatcgagcc
     3781 acgatcaact tcgccagagc gacctacttg ggtcgatagc agttccagcg ccttaaaccg
     3841 aagttttcat ttggcgaggc gggttttcgg cggaatggtg cgggttccgg gctgagtcat
     3901 attttggcgt gtccatgtag gggattccgg cggaaggcgg tttgtgattc gatggggcat
     3961 gttcatcgag accgtgcc
//
LOCUS       DATGEM010000183         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1087350, whole genome shotgun sequence.
ACCESSION   DATGEM010000183 DATGEM010000000
VERSION     DATGEM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087350"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VKQ27_05840"
     CDS             <1..438
                     /locus_tag="VKQ27_05840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014080.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLY88488.1"
                     /translation="LGGHGDTMVPLTRYSTVGGIPVPDLIKMGWTTQAKIDAIVQRTR
                     DGGGEIVKLLERGSAFYAPAASAVAMADAYLKDKKRVLPCAALLNGEYGMKDLYVGVP
                     VVLGAGGIERIVEVQLNAPEKAEFEKSCSAVRELVEAAKKLIG"
     gene            546..1742
                     /gene="sucC"
                     /locus_tag="VKQ27_05845"
     CDS             546..1742
                     /gene="sucC"
                     /locus_tag="VKQ27_05845"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HLY88489.1"
                     /translation="MNIHEYQAKDLLKRHGVAVPEGYVAWTPDEAVAAAAKLPGPVYV
                     VKSQIHAGGRGAGRFADAPSGKGGVRLARSPEEVRAATEAMLGHTLITRQTGPAGRTV
                     HRVYVEAGSDIARELYLSLLVDRATGRVTIVASTEGGMEIEEVAANHPDKIQRVTIDP
                     AAGISGFHGRRLAYALGLTGGQIAAFNRFLPALYRTFTELDCSIVEINPLVITKAGDV
                     IALDAKISFDDNALFRHPDLEKLRDEGEEDPKELEAAKYNLNYVALDGNIGCLVNGAG
                     LAMATMDIIKLFGMAPANFLDVGGGATKERVTAAFKIILSDPNVEGILVNIFGGIMRC
                     DVIAEGVVAAAREVSLSVPLVVRLEGTNVDLGKQILAKSNLPIIAADNLADAAQKIVT
                     AVKEAA"
     gene            1744..2619
                     /gene="sucD"
                     /locus_tag="VKQ27_05850"
     CDS             1744..2619
                     /gene="sucD"
                     /locus_tag="VKQ27_05850"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLY88490.1"
                     /translation="MAILVDANTRVITQGFTGAQGTFHSEQAIAYGTKVMGGVTPGKG
                     GTKHLDLPVFDTVWEARAATEATASAIYVPPPYAADAILEAIDAEIPLIVCITEGIPV
                     LDMVRVKRALTGSRSRLIGPNCPGVITPDACKIGIMPGHIHRRGSVGIVSRSGTLTYE
                     AVAQTTAVSLGQSSCVGIGGDPVKGTDFIEVLEMFLADDETKQLIMIGEIGGQSEIEA
                     ADFLAHNKVKKPTVGFIAGATAPPGRRMGHAGAVISGGRDTAAAKMEAMRAAGIHVAD
                     SPAALGSTMVEVLKG"
     gene            complement(2659..2832)
                     /locus_tag="VKQ27_05855"
     CDS             complement(2659..2832)
                     /locus_tag="VKQ27_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88491.1"
                     /translation="MTSEVDPEALAHRLAMIACATSDSATGVHLLEVVGELLEQAGFP
                     PASSLVGGNSSDG"
     gene            3008..3625
                     /locus_tag="VKQ27_05860"
     CDS             3008..3625
                     /locus_tag="VKQ27_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY88492.1"
                     /translation="MASSVAVIITATCEALVEAAAALIAEKGPAGFTIAEAARLAGVS
                     PGAPYRHFRDAEALLAEVALRGFQRFTEALVRAWNNGRPDLLRAFEALGRAYLAFARS
                     EPAYYAAMFEARIDRAAYPDLDAAGEQAFAVLRQAADALCATQPPANRPPALMVALHV
                     WSMSHGIASLFGRADASRRKLPMPPEDLLEAGVLIYLQSLGLARR"
     gene            complement(3636..4571)
                     /locus_tag="VKQ27_05865"
     CDS             complement(3636..4571)
                     /locus_tag="VKQ27_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HLY88493.1"
                     /translation="MRYLDQAAIRQSLSWDRLVPALRQVFAAGCVVPPRTVHTMELPG
                     DPDGTLLLMPAWQGGRWLVVKTAIVAPGNGARGRPAVSAVVQAFDGRTGEPRAVLDGG
                     EITARRTAATSAMVADTLARQDAAHLLVVGAGRIARNLVEAHCSVRNYQRVSIWARRP
                     EAASALAAELRNLSPMVKAAESLEAAARGADVISCATLSREPLVRGAWLKPGSHLDLV
                     GAYRHDMRETDDEAVRRASIIYVDTFEGALAEAGDLLQPMASGAITRTHISGELADLC
                     RGTGPARPDDSAITLFKSVGTAIEDYAAAVLAMEG"
     gene            complement(4740..5420)
                     /locus_tag="VKQ27_05870"
     CDS             complement(4740..5420)
                     /locus_tag="VKQ27_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HLY88494.1"
                     /translation="MLLAGGPIANAMPGSCNSANTFTISGPSFNQNFLIDGPSSVTPL
                     GGSGVFDCIRQQDKLFSAFSFGALPSGGTANLNFSAINGVDTHTISLVGSGLLNGQTY
                     QLGYNVAVTSSHAHLISTSSGILQSSGQASLAQTLLDNDGDTFNIAFTQTNGNNIAGN
                     SSTPLDPGVDWVDVTDVLTLLFPPGSDATGVANSFVEGVPEPAALLLLGVGVTALGLA
                     RRRSNRTD"
     gene            6006..6554
                     /locus_tag="VKQ27_05875"
     CDS             6006..6554
                     /locus_tag="VKQ27_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HLY88495.1"
                     /translation="MSGWANQGTYGNNSGSPWGPGAQPGWNPPWDAWRCWYAAKPLWI
                     AAMVLGFIFWWPVGLTLLFLGIWGKRMAFGGCGGWQRAGGNNGWHAQGGPPPWANWRS
                     FWSCGPANRQSASSGNHAFDEYRAETLRRLEDEQKEFGAFLERLRFAKDKAEFDQFMN
                     DRRNRPPAPPEGEGPQSGAPHA"
     gene            6813..>7110
                     /gene="sucA"
                     /locus_tag="VKQ27_05880"
     CDS             6813..>7110
                     /gene="sucA"
                     /locus_tag="VKQ27_05880"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438176.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HLY88496.1"
                     /translation="MAGVDILASAFSGANAAFLGEQYARWAANPHSVDPTFAELFSAL
                     NDDARAVLEEATGASWAPRHPREDDETPVSDRRRPQPPVLQSGEQVRAATIASLR"
BASE COUNT         1292 a         2355 c         2235 g         1228 t
ORIGIN      
        1 ctcggcggcc acggcgacac catggtgccg ctcacccgct actccaccgt cggcggcatt
       61 ccggtgcccg acctgatcaa gatgggctgg accacccagg ccaagatcga cgccatcgtg
      121 caacgcaccc gcgatggtgg aggcgagatc gtcaagctgc tggagcgtgg cagcgcgttt
      181 tacgcccccg ctgccagcgc cgtcgcgatg gccgacgcct acctgaagga caagaagcgc
      241 gtgttgccct gtgctgcgct gctcaacggc gaatacggca tgaaagacct ttatgtcggc
      301 gtgcccgttg tgctcggcgc cggtggcatc gagcgcatcg tcgaggtgca gttgaatgcg
      361 cccgaaaagg ccgagttcga aaagtcctgc agcgcggtca gggaattggt cgaagcagcg
      421 aagaagctga tcggttagcc ctgtcactgt aaggggcata gcgacgaagc aatcgcgatg
      481 aagcagccca acacccccgg gattgcttcg ccttcggctc gcaatgacag cgcgagaggc
      541 cgccgatgaa catccacgaa taccaggcga aagacctgct gaagcgccac ggcgtcgcgg
      601 tgcccgaagg ctacgtcgcc tggactcccg acgaagccgt cgctgccgca gcgaaactcc
      661 ccggccccgt ctacgtggtg aaatcccaga tccacgccgg cggccgcggc gccggccgtt
      721 tcgctgacgc cccgagcggc aagggcggtg tgcgcctggc ccgctccccc gaggaagtcc
      781 gcgccgcgac cgaggcgatg ctcggccaca cgttgatcac gaggcaaacc ggccccgccg
      841 ggcgcacggt gcaccgtgtc tacgtggaag ccggcagcga tatcgctcgc gagctctatc
      901 tgtccctgct ggtcgaccgt gccaccggcc gcgtcacgat tgtggcttcc accgaaggcg
      961 gcatggagat cgaggaagtc gctgccaatc accccgacaa gattcaacgg gtcacgatcg
     1021 atcctgctgc gggcatctcc ggcttccacg gccgccgcct tgcctacgcg ctcggcctca
     1081 ccggcgggca gatcgccgcg ttcaatcgct tcctgcccgc gctctatcgc accttcaccg
     1141 agctcgattg ctcgatcgtc gaaatcaacc cactcgtcat caccaaagcc ggcgatgtga
     1201 tcgcgctcga tgccaagatc agcttcgacg acaatgcgct gttccgtcac cccgacctgg
     1261 aaaagctgcg cgacgagggc gaggaagatc ccaaggaact cgaagccgcc aagtacaacc
     1321 tgaactacgt ggccctcgac gggaacatcg gttgcctggt gaacggcgcc ggactcgcga
     1381 tggcgacgat ggacatcatc aagctgttcg gcatggcgcc ggccaacttc ctcgatgtcg
     1441 gcggcggagc caccaaggaa cgcgtcaccg cggcgttcaa gatcatcctg tcggatccca
     1501 acgtcgaggg catcctggtc aacatcttcg gcggcatcat gcgttgtgac gtgatcgcgg
     1561 aaggtgtggt cgctgccgca cgtgaggtga gcctttccgt tcctctggtg gtccgcctgg
     1621 aaggcaccaa cgtggatctt ggcaagcaga tccttgcgaa gtccaacctg ccgatcatcg
     1681 cggccgacaa tctggccgac gccgcccaga agatcgttac cgccgtgaag gaggccgcct
     1741 gacatggcca tcctggtgga cgccaataca cgcgtcatca cacaaggctt cacaggcgcc
     1801 cagggcacct tccattccga gcaggcgatc gcctacggca ccaaggtcat gggcggcgtc
     1861 actcccggca agggcggcac gaagcacctc gacctgccgg tgttcgatac cgtgtgggaa
     1921 gcgcgtgccg cgaccgaagc cacggccagt gccatctacg tcccgccgcc ctacgcggcc
     1981 gatgcgatcc tggaagcgat tgatgcggag atcccgctga tcgtctgcat taccgagggc
     2041 atcccggtcc tcgacatggt ccgcgtgaag cgcgcgctca ccgggtcgag gtcacgcctc
     2101 atcggtccca actgccccgg tgtcattaca ccggacgcct gcaagatcgg catcatgccc
     2161 ggccacatcc atcgccgcgg cagcgtcggc atcgtgtccc gctccggcac cctgacctac
     2221 gaagcagtcg cgcagaccac ggcggtaagc ctcggccaga gcagttgcgt cggcatcggc
     2281 ggtgacccgg tgaagggcac cgacttcatc gaagtcctgg agatgttcct ggccgacgac
     2341 gagaccaagc agctcatcat gattggcgag atcggcggtc agagcgaaat cgaagccgcc
     2401 gactttctcg cgcacaacaa agtgaagaag ccgacggtgg gcttcatcgc cggcgcgacc
     2461 gctcctccag gccgccggat gggccacgct ggcgccgtaa tcagcggcgg ccgtgacacc
     2521 gccgccgcca agatggaagc aatgcgtgcc gccggcatcc atgtcgccga cagcccggcg
     2581 gcgctcggca gcacgatggt cgaggtgctg aaaggctagc agctcccacg ctgcgatcga
     2641 gccggggatg aaggcatgtc agccatcgga ggagtttccg ccgacgaggc tgctcgcggg
     2701 cggaaagcct gcctgctcca gcaattcccc gacgacctcc agcaagtgaa cgcccgtggc
     2761 ggaatcgctg gtcgcacagg caatcatcgc cagcctgtgc gccaacgctt cggggtctac
     2821 ttcacttgtc atggctgttc tccaaacgca tttggcgacg tcacctatcg tgggacctgc
     2881 cgcggcgctt tgtttttgtt gcaggtcact atgaccacag cggcgcggcg ccgcataggc
     2941 agctgatccc gttggatcgc cgccgcgccg aatgtaaaag tccttcacat gagctggacc
     3001 ggtcagggtg gcttcgagcg tcgcggttat catcacggca acctgcgagg cgctggtcga
     3061 ggccgccgct gcgctgatcg cggagaaggg ccccgccggc tttaccatcg cggaagcggc
     3121 gcgcctcgcc ggcgtcagcc ccggcgcacc ctaccgccac ttccgcgacg ccgaggcatt
     3181 gctcgccgag gtcgccctgc gtggcttcca gcgtttcacg gaagccctgg ttcgtgcctg
     3241 gaacaacggt cgcccggacc tgctgcgcgc gttcgaggca ctgggccgcg cctatctcgc
     3301 cttcgcccgc agcgagcccg cctactatgc ggcaatgttc gaagcacgga tcgaccgtgc
     3361 cgcttatcct gatctcgacg ctgccggcga acaggctttc gccgtgctgc gccaggcagc
     3421 cgacgcgctg tgtgccactc agccgccagc caaccggccg ccggcgctca tggtggcgtt
     3481 gcatgtctgg tcgatgtcgc acggcatcgc gtcgttgttc ggccgtgccg acgcctcacg
     3541 ccggaagctg ccgatgccgc ccgaggacct gctggaggcc ggggtactga tctacctgca
     3601 aagcctgggg ctggcgaggc gataagagcg gcggctcagc cttccattgc gagcactgcc
     3661 gcggcgtagt cttcgatcgc ggtaccgacc gacttgaaca gcgtgatcgc gctgtcgtcc
     3721 ggccgcgcgg gacccgtgcc gcggcacaga tcagccagtt cgcccgagat gtgcgttcgg
     3781 gtgatcgcgc cggaggccat cggctgcaac aggtcgccgg cctcggccag cgcgccctcg
     3841 aatgtatcga cgtagattat cgaggcgcgg cgcaccgcct catcatccgt ctcgcgcatg
     3901 tcgtgccgat aggcgcccac gaggtccagg tgtgagcccg gcttcagcca cgcgccgcgc
     3961 accagcggtt cgcgcgagag tgtagcgcaa ctgatcacgt cggccccgcg cgccgctgct
     4021 tccagtgatt ccgctgcctt caccatcggc gacaggttgc gcagctccgc ggccagcgcc
     4081 gacgccgcct cgggccgccg ggcccagatc gagacgcgtt ggtagttccg aacgctgcaa
     4141 tgcgcctcca ccaggtttcg cgcgatccgt cccgctccga cgactagcag gtgggccgcg
     4201 tcctgccgcg ccagcgtgtc ggccaccatc gccgaggtcg ctgccgtgcg gcgcgcggtg
     4261 atttcacccc cgtccagcac cgcgcgcggc tcgccggtgc gcccgtcgaa cgcctgcacg
     4321 accgcgctta cggccggccg gccgcgcgcg ccgttgccgg gcgccacgat ggcggtcttc
     4381 acaaccagcc accggccgcc ctgccacgct ggcatcagca gcagcgtgcc gtccggatcg
     4441 cccggtagtt ccatcgtatg cacggttcgc ggaggtacca cgcagccagc ggcaaacacc
     4501 tgtcgcagcg ccggcaccag tcgatcccat gacagggact ggcggattgc agcctggtcg
     4561 agataacgca ttggcacaga ctagcatgtc tgccgcctgg aagcggagaa gcttgtgccg
     4621 tcatcaccgg tgcggttgca gaaatggcga gtgcgaaagc aggaacccgg cgcgatctct
     4681 gtcgatcgct ccgggttccc gcactcgcgg tgtgattacc gagttagtgc atcaatcgtt
     4741 cagtcagttc ggttgctgcg gcggcgcgcc aaaccaagtg ccgtcacgcc tactcctagc
     4801 agcagcaatg cggcaggctc cggtacccct tcgacgaatg agttggcgac gccggtggca
     4861 tcggagccgg gaggaaacag caaggtcaac acgtcggtca cgtcgaccca atcgacacct
     4921 ggatcgagcg gggtgcttga gttgccagcg atgttgttgc cgttcgtttg cgtgaaggcg
     4981 atgttgaacg tgtcgccatc attgtccaga agtgtctgag caaggctcgc ctggccggat
     5041 gactgcaaga tgccagatga cgtcgaaatc aagtgcgcat gtgacgaagt caccgcgaca
     5101 ttatagccca gttggtatgt ctggccgttg aggagcccgc tcccgaccag cgatatggtg
     5161 tgggtgtcaa cgccgttaat ggcggagaag ttcagattgg ccgtgccacc actcgggagg
     5221 gctccaaagc tgaacgcgct gaacaacttg tcctgttgcc gaatgcagtc gaagacaccc
     5281 gacccgccca gcggcgttac cgacgatggc ccatcgataa ggaagttctg attaaaactt
     5341 gggccggaga tcgtaaaggt attggcactg ttacaacttc cgggcatcgc gttggcgatc
     5401 ggcccgccgg ccaaaagcac gacagccgcg gctgccaggg tgccgtggaa tgatgcagct
     5461 ctcatcactt ggaattcccc tcttggaaat gacgcgcact cagacgcgat cggaacacac
     5521 aggagcagtt tccgtaccaa ataggttaac ggtggaataa caatcgatta gcgcacggtc
     5581 aaagaccggc attctctggt gttgtaagaa tctccgacaa actgcagtca aggccgtagc
     5641 ttattgacca tagacttgcg cgatctaaac cgcgctcagt cgagtgtttg agcgctaata
     5701 gattaattgg agccgccggc tcatctatat taacctctgt taaggattgt caggccaatt
     5761 tatggcatcc aagaataaac gataatagcg acggctcgct ctgggctggg ttgcgatgtt
     5821 cggcttgtat acacggaccg ccggtctgca cggatccggg cccgctcgcc ggcactcagc
     5881 ggcgttgcgt caaggggcat gaggccctgt tgcttgcagg gctgttgaag cggcgatttc
     5941 cccttgacgc cgctgtgcgc cgcacccaat atgtaaatgt atcttacatt cacatgaggt
     6001 ccaccatgtc aggttgggca aaccaaggta cctacggcaa caacagcggc tcaccctggg
     6061 gtcccggcgc acagccgggc tggaatcccc catgggacgc ctggcgctgc tggtatgcgg
     6121 ccaagccgct gtggatcgcc gcgatggtcc ttggcttcat tttctggtgg ccggtggggc
     6181 tcactctgtt gttcctgggc atatggggaa agcgcatggc attcggcgga tgtggcggct
     6241 ggcagcgtgc cggcggaaat aatggctggc atgcgcaggg tggtcctccg ccttgggcaa
     6301 actggaggag cttctggagc tgcggtccgg ccaatcgcca gtcggcttcc agcggcaacc
     6361 acgccttcga cgaataccgc gccgagacgc tgcgccggct ggaggacgag cagaaggagt
     6421 tcggcgcctt cctggagcgc ctgcgcttcg ccaaggacaa ggctgagttc gaccagttca
     6481 tgaacgaccg ccgcaaccgc ccgcccgcgc cgccggaagg cgagggccca cagtccggcg
     6541 cgccgcacgc ctgacagcgc accaagggac gaagttgtaa cgcgccttgc ggggggacgc
     6601 ccgttacgtc ccacgcaagg cgctggtttt gttaaggtgt ttcgctcacc ccagccctgc
     6661 gcctcattcg cttgcaaacc gcccacaccg gtcgcatttc gccagtgcaa ccgggtgaca
     6721 taaccgcaaa ccggcccgga gccgccgaac aggggttccg ttgaccgttc tgtgcgtcta
     6781 tgccgcgcgc acagattaag gaagctgacg taatggctgg cgtggacatc ctggcctccg
     6841 ccttcagcgg cgcgaacgcc gcgttcctcg gtgagcaata cgcccggtgg gccgccaatc
     6901 cgcactctgt cgatccaacc ttcgccgagc tgttcagtgc cctgaacgac gacgcgcggg
     6961 cggtgctgga ggaggccact ggcgcctcct gggcaccccg gcatcctcgc gaggacgatg
     7021 agacgccagt ctcggatcgc cgcaggccgc aacctccggt gctgcagtcc ggtgagcagg
     7081 tccgcgccgc caccattgcc agcctccgcg
//
LOCUS       DATGEM010000184         9808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_405008, whole genome shotgun sequence.
ACCESSION   DATGEM010000184 DATGEM010000000
VERSION     DATGEM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405008"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="dctP"
                     /locus_tag="VKQ27_05885"
     CDS             complement(<1..564)
                     /gene="dctP"
                     /locus_tag="VKQ27_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HLY88497.1"
                     /translation="MIELHFAGYQPARSVHTRGLHALRDGVARHAGAALSIKVTDSVV
                     SLGRKAADLLSMVEAGELDGCYFASSYLAARVPALGVFDAPFQAGTRETVFATLDPAL
                     ADQVAATTGYRVIGWWDNGIRHLSNAVRPIRSPADCNGLRLRTLDNAQHQAAFRRCGF
                     VPVFIDVAGLARAVADGTVDAQENPLTN"
     gene            570..1403
                     /locus_tag="VKQ27_05890"
     CDS             570..1403
                     /locus_tag="VKQ27_05890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HLY88498.1"
                     /translation="MYGAAKVILPWWGMAFRLLFWRKPRIGVIELHGLISPRPGTVSM
                     GTMAPLIERAFRAAQGQPVILDIESPGGSPVQSDLIASLIRRRAEERKVRVHAVVREV
                     GASGGYWLACAADEIIANPMSIVGSIGVRGGGFGFPDMLARLGVERRLYTAGTNKARL
                     DPFSPEKPEDVAFVQDLLDALHDRFKGWVRTRRAGKLKVEEAALFDGSFMLGEKALEV
                     GLIDGFGDVDSVVRAIGGEKAKSRVFRPKKRGLGRFVPGLMDAVLDAVEERSWRSMLG
                     R"
     gene            complement(1418..1627)
                     /locus_tag="VKQ27_05895"
     CDS             complement(1418..1627)
                     /locus_tag="VKQ27_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88499.1"
                     /translation="MLSLLVDKGWTGDRRVVERPTMNSPWLLIYGAAIAVAALLIIGG
                     LVLATADFGAHSHPPPIIKERIRLM"
     gene            1805..2311
                     /locus_tag="VKQ27_05900"
     CDS             1805..2311
                     /locus_tag="VKQ27_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HLY88500.1"
                     /translation="MPVTIGLGATGDDVKRVQRALARQLLWSPFGPITGVYDATLEAS
                     VKLFQQANGLPVTGTVDGATWAKLPGYKEASPTLRVGDTGPVVAWLQQALKGQDVQIL
                     FTPYAGAIDGIFGPLTEAAVRALQTWAGATVDGIVSDNTWFTWMTPGSAQQLTLEAAC
                     GLLRGIGP"
     gene            complement(2416..2856)
                     /locus_tag="VKQ27_05905"
     CDS             complement(2416..2856)
                     /locus_tag="VKQ27_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88501.1"
                     /translation="MRAEPNNPDVGALTWDVDPKRHVTVLRLAGDLDGQAVRARIEAF
                     WQAHPEDIANHCVVDMRNYTGDLGYDDLTTIAQQWRKFARGRDAGRGTAIVTNDRFAR
                     LLMRAVALLFPTRRFALFADMDEATRWLGTISQDSSPYHNGAVK"
     gene            complement(2887..3633)
                     /locus_tag="VKQ27_05910"
     CDS             complement(2887..3633)
                     /locus_tag="VKQ27_05910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88502.1"
                     /translation="MADRFAVITGSGTGIGRSSALALMNDGWSVALCGRRKEMLEETA
                     SMKKAGRALVIPTDVTDPEQVEKLFATVKAEFGRLDMLFNNAGGGTPTTNFGDFTYEM
                     WQRVVQVNLNACFLCANHAFKIMRDQSPRGGRIINNGSISAHVPRVGSVAYTATKHAI
                     TGLTKSIALDGREYDICSCQIDIGNAATPLTARTTNGQMQPYGQMAVEPRMDVDHVGQ
                     QVLFMSNLPLESNVQFVTIMATKMPFIGRG"
     gene            3734..4741
                     /locus_tag="VKQ27_05915"
     CDS             3734..4741
                     /locus_tag="VKQ27_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY88503.1"
                     /translation="MPRFAAIGLDHRHIYDLTHDLLDEGAECVGYNPDTTDLRVLAGF
                     RKRFPHVPEATTERLLDDPSVDFVVIAARPCDRAEMAIKAMRHGKDVMVDKPGITTLE
                     QLVAVERAVAETKRIWSVCIGRLASPAVQEALRIVRSGELGRVVHTTSLAPHRLNRTL
                     RPAWFFDKPAYGGIINDIGVHSIDQFLAFADADDAGIEHAAIGAFGTEPEGFEDFAEI
                     MLATQSVRGYVRLDWFTPDGLPTWGDGRFFVVGTQGTLELRKNMDIEGREGTDHLFVA
                     NRSATRYVDCSALPVMYYRDFLRDIVERTETAMPQRLTFNVCRLALQAQSQAVRYTAR
                     R"
     gene            4741..5739
                     /locus_tag="VKQ27_05920"
     CDS             4741..5739
                     /locus_tag="VKQ27_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY88504.1"
                     /translation="MIGMAVIGLGNALQPHARALVDLKDRVRVVWAAASSEARLKDVE
                     QRYGFPVTTDVTRAITDPAVDAVMVLTPANAHLPIAEAAFGAGKHVLCEKPLEVTVAR
                     GETLVAAGRKARRRLGICLQHRFRAASQRLHAVLAAGALGEVQVATLMVPWWRPQSYY
                     DEPGRGVLARDGGGVLMTQAIHSLDLFRWLVGIERVEAAQVSTTALHRMETEDYASAL
                     VRLGNGAAGTIVATVAAYPGSPEFMHIIGSKGTGRLDGGNLRLAFVDGQEEVLTDSAG
                     TGSGASVMAFSHEAHRAVLTDFLDAIEQDRDPAITGEEALATQRVIDAILDVGKGA"
     gene            5736..6263
                     /locus_tag="VKQ27_05925"
     CDS             5736..6263
                     /locus_tag="VKQ27_05925"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HLY88505.1"
                     /translation="MKNTALLALGISAALVSGAIAATLDVKPGLWEVSTQGEASGAPP
                     IPPQLLAQMSPQQREQMMAAMGRMNQPNVSRSCVTQKMLDRGMSFDRPSNDQCTQTVT
                     GSTSHSLDVKVVCTGERQQKTSGTMHMDATSRESFSGAFNMVSTDGANTMTMKRTMQG
                     KWLGSDCGNVKPHEE"
     gene            6276..6758
                     /locus_tag="VKQ27_05930"
     CDS             6276..6758
                     /locus_tag="VKQ27_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HLY88506.1"
                     /translation="MQDLLPLAERLGARLKARGDTIAIAESSTGGLVSAALLSVAGAS
                     AYFRGGSVIYTHYARSGFLEIPNPLPDGMRASSEPYAALLAATVRSRLQSTWGLSETG
                     ATGPTGNRYGDAAGHTCIGLEGPGFSKAITLETGSGDRVANMRAFARRALELLGEALG
                     "
     gene            complement(6852..>9808)
                     /locus_tag="VKQ27_05935"
     CDS             complement(6852..>9808)
                     /locus_tag="VKQ27_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HLY88507.1"
                     /translation="FRHGGERPLDLHVTNIVITDQSGVRRMSVPGADLTVSLYELLQG
                     RVALRTVEVDGARLTVIRAADGTLSADIGDLAEPDTAPPPDATPIADLLAELARPART
                     DRDATSTLLGQLREVRIHDARVTIIDRNLGVTWRAPQAQIDLARHPAGGVEGTADLTL
                     ALGQQQAHLALGATLTADAAETHLRARITPFVPSALAQSAPSLAALAALDAPVSGQAA
                     VDLDRHLGFRHARFDLHAGPGNARIGNGAVPFVDASLAGSATPDAVNLRTLRVTLRGR
                     ATGPDTHLETHGSVQFSPDRISAGFSADLDQVDFADLPVLWPEGVGGNARDWLLENIT
                     AGTARNGHVDVGIEASSNLADVAITRATGTLDGVGLQVHWLRPVPPIDNGRAQLRIID
                     PDTLEIMVAGGRQILRNQKGNAAGIQIRDGRMRITGIMQARQIGTIDANISTSLPDAL
                     ALLREPRLRLLDRHPIDLKDPSGQASVKLAVSLPLDKDVAMDDIAIRAQAHLDDVHLG
                     NVVAGRDLDQGSFDLDANADGMKLKGKATLAAIPATLEAAMDFRAGPPAQAVQTVTIS
                     GQASAAQLASAGLDTTPVVAGPMQLTATLTERRNGSGELAISSDLSAAELSVSALEWQ
                     KPRGSPVKASARLTLDHDRLSRLDAVQIDGDGISVRGRGTFSAGKLTQVQLDRLALGR
                     TVADAVVRLPESSTAPIVVTCNGTVVDLAARMERKTPPHQPEKNEPPPGPRWTLDAKF
                     TRVLMAHGATFGGVVLHAENDGRVFRQLRLEGRTSAQGPFLLQIVPDGADRRLVVTAA
                     DAGELLRGLDYVKTMQGGKLSIEGNYDDAKPGRPLAGNAHIEDFRIQNAPALGKLLQA
                     MTLYGLADVVRGPGLGFTRMEAPFRLTDDSLAIDDARAFSSSLGLTVKGQINLDEQVI
                     DMEGTIVPAYFFNSLLGHIPLVGKLFSPERGGGVFAASYTVRGALDDPQVSVNPLAAL
                     TPGFLRGVFRLF"
BASE COUNT         1668 a         2873 c         3513 g         1754 t
ORIGIN      
        1 gttggtgagc ggattttcct gggcatccac ggtcccgtcc gccactgcgc gtgccaggcc
       61 ggcgacgtcg atgaagaccg ggacgaagcc acagcggcgg aaggcggcct gatgctgggc
      121 gttgtccagg gtgcgcaggc gcaggccgtt gcaatcagca ggcgagcgga tcgggcgaac
      181 ggcattgcta aggtggcgga tgccgttgtc ccaccagccg atcacgcgat aaccggtggt
      241 tgcggccacc tggtcggcca gcgcagggtc gagggtggca aatacggttt cccgtgtgcc
      301 ggcctggaat ggcgcgtcga acacgccgag cgcgggcacc cgtgcggcca ggtagctcga
      361 ggcaaaatag cagccgtcga gctcgccggc ctccaccatg gacagcagat ccgcggcctt
      421 gcggcccagg ctgacgacac tgtcggtcac cttgatcgag agcgcggcgc cggcgtggcg
      481 cgcgacgccg tcgcggagtg catgcaggcc gcgcgtgtgg acggatcggg cgggctgata
      541 gccggcgaaa tgcagttcga tcatgtcagg tgtatggcgc agcgaaggtg atcttgccat
      601 ggtggggcat ggcattccgg ctgttgttct ggcgcaaacc gcgtatcggc gtgatcgagc
      661 tgcatgggct gatatcgccg cggcctggca ctgtgagcat gggcacgatg gcgccgctga
      721 tagagcgcgc gttccgggcg gcgcaggggc agccggtgat tctggacatc gagagtccag
      781 ggggctcgcc ggtgcagtcg gacctgatcg cctcgctgat ccggcggcgg gccgaggagc
      841 gcaaggtgcg tgtgcatgcg gtggtcaggg aggtgggagc gtcgggcggt tactggctgg
      901 cctgtgccgc ggatgagatc atcgccaatc cgatgagcat cgtgggatcg atcggtgtgc
      961 gtggcggggg atttggcttt cccgacatgc tcgcgcggct gggggtggag cggcgacttt
     1021 ataccgcggg gaccaacaag gcccggctgg acccgttcag tccggaaaag ccggaggacg
     1081 ttgcgttcgt gcaggatctc ctggatgcgc tgcatgatcg gttcaagggc tgggtacgga
     1141 cgcgacgagc ggggaagctg aaggtggagg aggcggcgct gttcgacggc agcttcatgc
     1201 tgggggagaa agccctggag gtggggctga tcgatggctt tggggatgtc gacagcgtcg
     1261 tgcgcgccat cggcggcgag aaggcgaaat cgcgggtgtt ccgaccgaag aagcgcgggc
     1321 tcggacggtt tgtgccgggg ctgatggatg cggtgctgga tgctgttgaa gagcgaagtt
     1381 ggcgatcgat gctaggccgc tgacacagcg gcgcgtccta catcaacctg atgcgctctt
     1441 tgataattgg cggcggatgg gagtgtgcgc cgaaatcagc cgtcgccagc acaaggcctc
     1501 caatgatgag gagggcggct accgcgattg ccgcgccgta aatcagcagc cacgggctgt
     1561 tcatagtggg tcgctccacg acacggcggt cgccggtcca gcccttatcg acgagaaggc
     1621 tcagcatatt ccctctctcc tgttctcggt gcgcggacga ttgcgcaccc gacactgcgc
     1681 gatggcctat gcggctgcta tgacttgcgt cacagcgcgg agcagtcgag acagcgcagt
     1741 gtcgcgttcc gttacgatga gctgacctgt tggtttcggc gggaacaatg caaaggggga
     1801 gaaaatgcct gtgacgattg gccttggtgc cacgggagac gacgtcaagc gcgtgcaaag
     1861 ggcgctcgcg cggcagttgc tctggagtcc attcggtcca atcaccggcg tgtacgatgc
     1921 cacgctcgaa gcgtcggtca agctgttcca gcaggccaac gggctgccgg tgaccgggac
     1981 ggtggacggc gcgacctggg caaagctacc aggctataag gaagcctcgc caacgctgcg
     2041 tgtcggtgac accgggccgg tcgtcgcatg gttgcagcag gccctgaagg gccaggacgt
     2101 tcagatcctt tttacgccct atgccggcgc gattgatggg atcttcggac cgttgaccga
     2161 ggcggctgtc cgcgcgctgc agacgtgggc cggtgcgacg gtggatggga ttgtcagcga
     2221 caatacgtgg tttacgtgga tgacgccggg aagcgcccaa cagctgacac tggaggccgc
     2281 gtgcgggctg ttgcgtggga ttggcccgta gacgcgagtg tcgtggcaat ttgtcgctca
     2341 tctactttgg ttcgcgtgga aggaaaagac gtggatggca ggccttcgcc cgccatgacg
     2401 atgggaaagc gccagtcatt taacggctcc gttatgatac ggagacgaat cctgagagat
     2461 tgtgccgagc catcgcgtgg cttcgtccat gtcagcgaat agcgcaaacc ggcgcgtggg
     2521 gaacagcagc gcgacggcgc gcatgagtaa ccgtgcaaag cgatcgttcg tcacgatcgc
     2581 tgtgccacgt ccggcatctc gtcctcgggc aaactttcgc cattgctgtg cgatcgtggt
     2641 caggtcgtcg tagcccagat cgcccgtgta gttgcgcata tcgacaacgc aatgattggc
     2701 gatgtcctcg gggtgggcct gccagaacgc ttcgatccgc gcgcggacag cctggccatc
     2761 caggtcaccg gccaatcgca ggacagtgac atgacgcttg ggatcgacgt cccatgtcag
     2821 tgcgccgacg tcgggattgt tcggttcggc tcgcaatgac actggtgagg gccgggcggt
     2881 gcgtggtcag ccccgaccga tgaacggcat cttcgtcgcc atgatggtga cgaactgcac
     2941 gttcgactcc aagggcaggt tggacatgaa cagcacctgc tggccgacat ggtccacgtc
     3001 catccgtggc tcgactgcca tctggccgta gggctgcatt tgaccattgg tggtgcgcgc
     3061 tgtcagcggc gtggctgcgt tgccgatgtc gatctggcag gagcagatgt cgtattcgcg
     3121 gccgtcgagc gcgatcgact tcgtaaggcc ggtgatcgcg tgcttggttg ctgtgtaggc
     3181 gacggaaccg acgcggggaa catgcgccga gatcgagccg ttgttgatga tgcgtccgcc
     3241 gcggggtgac tggtcacgca tgatcttgaa cgcatggttg gcgcagagga agcacgcgtt
     3301 caggttcacc tgcacgacgc gctgccacat ctcgtaggtg aaatcgccga agttggtggt
     3361 gggcgtgccg ccgcctgcat tgttgaacag catgtccagg cggccgaatt ccgccttcac
     3421 cgtggcgaag agtttttcga cctgctctgg atcggtaacg tcggtcggaa tcaccagggc
     3481 gcggccggct ttcttcatgc tggcggtttc ctccagcatc tctttgcggc ggccacacag
     3541 cgcgaccgac cagccatcat tcatcagtgc cagcgccgat gagcgcccga tgccggtgcc
     3601 ggagccggtg atgaccgcga aacggtccgc catggtgttt ctcccctctc ggttcatacg
     3661 tgatggcgcg cattgtggac cttggttgcc gaacggggaa gatggtgcaa gcgtgcgtgc
     3721 gggaggggtt tgcatgccac gctttgccgc gatcggactg gatcatcgcc atatctatga
     3781 tctgacgcac gacctgctgg acgaaggcgc ggagtgcgtc ggctacaatc cggataccac
     3841 cgatttgcgc gtgctggcgg ggttccgcaa gcggttcccg cacgtgccgg aggcgacgac
     3901 ggaacgattg ttggacgatc catcggtcga tttcgtggtg atcgcggcac ggccatgcga
     3961 tcgggccgag atggcgatca aggcgatgcg acacggcaag gatgtgatgg tggacaagcc
     4021 tggcatcacc acgctggagc agcttgttgc ggtggaacgc gcggtggcgg agacgaagcg
     4081 catctggtcg gtgtgcatcg ggcgcctggc gtcgccggcg gtgcaggagg cgttgcggat
     4141 cgtgcggtcg ggggagctgg ggcgcgtggt gcacacgacg tcgctggcgc cgcatcggct
     4201 gaaccgcacg ctgcggcctg cgtggttctt cgacaagccg gcgtatggcg ggatcatcaa
     4261 cgacatcggc gtgcactcga tcgatcagtt cctggcgttc gccgatgcgg atgatgcggg
     4321 gatcgagcac gcggcgatcg gtgcgttcgg caccgagccg gagggcttcg aggatttcgc
     4381 cgagatcatg ctggcgacgc aatcggtgcg cggctacgtg cggctggatt ggttcacgcc
     4441 ggatggcttg ccgacgtggg gcgacgggcg gttcttcgtg gtggggacgc aagggacgct
     4501 ggagttgcgg aagaacatgg atatcgaggg gcgcgaggga accgatcacc tgttcgtcgc
     4561 gaaccggtcc gcgacgcggt acgtcgattg cagcgcgttg ccggtgatgt actaccgcga
     4621 cttcctgcgg gacatcgtag agcggacgga aaccgcgatg ccgcagcggc tgacgttcaa
     4681 cgtctgccgg ctggcgttgc aggcgcagtc ccaggcggtt cgctacacgg cgcgccgctg
     4741 atgatcggga tggcggttat cgggctgggc aatgcgctgc agccgcatgc ccgggcgctg
     4801 gtcgacctga aggatcgcgt gcgcgtggtt tgggcggcgg cgagcagtga ggctcggctg
     4861 aaagatgtcg agcagcgcta cggatttccg gtcacgacgg atgtgacgcg ggcgatcacc
     4921 gacccggcgg tggatgctgt gatggtgctg acaccggcca acgcgcatct gccgatagcc
     4981 gaggcggcgt tcggcgcggg caagcatgtg ctgtgcgaga agccgctgga agtgacggtg
     5041 gcacgcggcg agacgctggt cgcggctggg cggaaggcaa ggcggcggct ggggatctgt
     5101 ttgcagcatc gattccgggc ggcgagccaa cggctgcatg ccgtgcttgc tgcgggcgcg
     5161 ctgggcgagg tgcaggtggc gacgctgatg gtgccgtggt ggcggcctca gtcttactac
     5221 gacgaaccgg ggcgcggcgt gctggcgcgc gatggcggcg gcgtgctgat gacgcaagcg
     5281 atccattcgc tcgatctgtt ccgctggctg gttgggatcg agcgtgtgga ggcggcgcag
     5341 gtatccacga ccgcattgca caggatggag accgaggatt acgcctcggc gttggtgcgt
     5401 cttggcaacg gtgcggcggg aacgatcgtg gcgacggtgg cggcttatcc gggcagtccg
     5461 gagttcatgc atatcatcgg ctcgaaaggg acgggacggc tggatggcgg caacctcagg
     5521 ctcgcgttcg tcgatgggca ggaggaggtg ctgactgaca gcgccggtac cggcagtggc
     5581 gccagcgtta tggcgttctc acacgaggca catcgcgcgg tgctgaccga ctttcttgat
     5641 gcgatcgagc aggatcgcga tccggcgata accggcgagg aagcactggc gacgcagcgg
     5701 gtgatcgatg caatcctgga tgtaggaaag ggcgcatgaa gaacactgcg ttgctggcgc
     5761 tgggtataag cgcggcgctg gtcagcggcg cgattgcggc gacgctggat gtgaagccgg
     5821 gcctgtggga agtgagcacg caaggtgagg catcgggggc gccgccgatc ccgccgcagt
     5881 tgctggcgca gatgtcgccg cagcagcgcg agcagatgat ggcggcgatg gggcggatga
     5941 accagccgaa cgtgtcacgc tcctgcgtca cacagaagat gctggatcgc ggcatgagct
     6001 ttgatcggcc gagcaacgat cagtgcacgc agacggtgac cggcagcacg tcccattcgc
     6061 tggacgtgaa agtcgtgtgc acgggcgagc gacaacagaa gaccagcggc acgatgcata
     6121 tggatgcgac cagccgggag agcttttccg gtgcgttcaa catggtctcg acggatggcg
     6181 cgaatacgat gacgatgaag cggacgatgc agggcaagtg gctcggcagt gattgcggca
     6241 acgtgaagcc gcacgaggaa tagggagaac gagcgatgca ggacttgctg ccgttggcgg
     6301 aacggctggg tgcgcggctg aaggcgcggg gtgatacgat cgcgatcgcg gagtcctcga
     6361 cgggagggct ggtatcggcc gcgttgctgt cggtggcggg tgcgtcggcg tatttccgcg
     6421 gggggagcgt gatctacacg cattatgcgc ggtcgggatt cctggagatc cccaatccgc
     6481 tgccggacgg aatgcgggct tcgtcggagc cctatgcggc gctgctggcg gcgacggtgc
     6541 gcagccggtt gcagtcgact tggggattga gcgagacggg agccacgggc ccgacgggca
     6601 atcgctatgg cgatgcggcg gggcatacgt gcatcgggct cgaggggccg ggattctcca
     6661 aggctatcac gctggagacg gggagcgggg atagggtggc aaatatgcgg gcgttcgcgc
     6721 gacgggcgct ggagctgctg ggcgaggcgt tggggtaggg gttcttgtgt cgttgcggag
     6781 gggcgagaag gtgggaagca atcacgttgg ggattgcttc gtcggctgcg cctcctcgca
     6841 atgacaggcg ttcagaacag tcggaatacg cctcggagga aaccgggcgt cagtgccgcc
     6901 agcgggttca ctgacacctg cggatcgtcc agcgcgccgc ggacggtata gctcgctgca
     6961 aatacaccgc cgccgcgttc gggactgaac agcttgccca ccagcggaat gtgacccagc
     7021 agcgaattga agaagtacgc gggcacgatg gtgccttcca tatcgatcac ctgctcatcg
     7081 agattgatct gtcccttcac cgtcaagcca agcgacgaac tgaaggcgcg ggcatcatcg
     7141 atcgcaagtg agtcatcggt cagccggaac ggtgcctcca tccgtgtgaa gcccagcccc
     7201 ggcccgcgca ccacgtcggc cagtccatac aatgtcatcg cctgcagcag cttgccgagc
     7261 gccggggcat tctggatgcg gaagtcctcg atatgcgcgt tgccggccag cggcctgcct
     7321 ggcttcgcgt cgtcgtaatt gccctcgatc gacagcttgc cgccctgcat cgtcttcaca
     7381 taatccaatc cgcgcagcag ctcgcctgca tccgccgcgg tgacaacaag ccgccggtcg
     7441 gcgccatcag gaacgatctg gagaaggaac ggcccctgcg cgctggtgcg tccttccagc
     7501 cggagttgcc tgaacacgcg cccatcattt tctgcgtgca gcacgacgcc gccaaaggtc
     7561 gctccgtgcg ccatcagcac gcgggtgaat ttcgcatcca atgtccaacg cggcccgggc
     7621 ggcggctcgt tcttctcagg ctggtgcggc ggcgtcttcc gttccatgcg cgcggccaga
     7681 tccaccacgg tgccgttgca ggtcaccacg atcggggcgg tgctgctttc cggcaatcgc
     7741 acgacggcgt ccgccacggt acgccccagc gccagccggt ccagttgcac ctgcgtgagt
     7801 ttgcctgcgc tgaatgtgcc gcgcccacgc acagagatgc cgtcgccatc aatctgaact
     7861 gcatcgagcc gcgacaggcg atcatgatcc aaggtcaggc gtgcggatgc cttgaccggg
     7921 gatccgcgcg gcttttgcca ctccagtgcc gacacgctca gctccgccgc ggacaggtcg
     7981 gagctgatcg ccagctcacc cgaaccgttg cggcgttcag tgagagtcgc ggtcaactgc
     8041 atcgggccgg cgaccaccgg cgtcgtatcc aatccagcac ttgcgagctg cgcggcactg
     8101 gcctggccac ttatggtcac ggtctgcact gcctgagccg gcggaccggc gcgaaagtcc
     8161 atcgccgctt ccagcgtcgc cgggatcgca gccagcgtcg ctttcccctt cagcttcatc
     8221 ccatcggcat tcgcgtccag atcgaagctt ccctggtcca ggtcgcgccc ggcaacgacg
     8281 ttgcccagat gcacatcatc cagatgcgcc tgggcgcgaa tggcgatgtc atccatggcg
     8341 acatccttgt ccaagggcaa cgacacggcg agtttcacgc ttgcctggcc ggatgggtcc
     8401 ttcaggtcga tcgggtgccg atcaagcagc cgcaaccgtg gttcgcgcag caatgccagc
     8461 gcgtcgggca aagaggtgct gatattggcg tcgatggtgc cgatctgacg tgcctgcatg
     8521 atgcccgtga tgcgcatccg accatcgcgg atctgaatgc cggccgcatt gcccttctgg
     8581 ttgcgcagga tctgccgccc gccagcgacc atgatctcga gcgtgtcagg atcgatgatg
     8641 cgcaactgcg cccggccgtt gtcgatcggc ggcaccgggc gcagccagtg cacctgcaat
     8701 ccaaccccgt ccagtgtgcc ggtggcccgt gttatcgcca cgtccgccag gtttgatgac
     8761 gcctcgatac ccacatccac gtggccgttg cgagctgttc ctgcggtaat gttctccagc
     8821 agccagtcac gtgcattgcc gccgaccccc tccggccaaa gcaccggcag atcggcaaag
     8881 tcgacctgat cgagatcggc cgagaagccg gcactgatgc gatcagggct gaattgcaca
     8941 ctaccgtgtg tttccagatg cgtatcgggg cctgtggcgc ggccgcgcag cgtgacgcgc
     9001 agcgtgcgaa gattcacggc gtccggtgtg gctgagccgg ccagcgacgc atcgacgaag
     9061 ggaaccgcgc cgttgccgat ccgcgcgttg ccggggcccg catgcaggtc gaaccgcgca
     9121 tggcggaagc cgaggtgccg gtcgagatcg accgccgcct ggccgctcac cggcgcatcg
     9181 agcgccgcca gcgcagcgag cgacggcgcg ctttgcgcca gggcacttgg cacgaacggc
     9241 gtgatgcgtg cgcgcaggtg tgtctcggcg gcatctgctg tcagcgtcgc gccaagggcg
     9301 agatgtgcct gttgttggcc gagcgcgagc gtgaggtcgg cggttccttc cacgccgccg
     9361 gccggatggc gcgcgaggtc gatctgggcc tgcggcgcac gccacgtcac gccgaggttg
     9421 cggtcaatga tggtgacccg cgcgtcgtgg atccggacct cgcgaagctg cccgagcagt
     9481 gtgctggttg catcccgatc ggtgcgcgcc ggccgggcaa gttcagccaa caggtccgcg
     9541 atcggcgtgg catcgggcgg cggcgcggtg tccggttctg ccagatcccc gatatccgcg
     9601 ctcagcgtgc catccgccgc ccggatgacc gtcagacgcg cgccatcgac ctcgacagtg
     9661 cgcagcgcga cccgtccctg cagcaattcg tacaacgaca ccgtcaaatc cgcgccaggc
     9721 acggacatcc ggcgcacacc ggactgatcg gtgatgacga tgttggtgac atgcaggtcc
     9781 agtggccgct cgccgccgtg acggaaac
//
LOCUS       DATGEM010000185         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_630847, whole genome shotgun sequence.
ACCESSION   DATGEM010000185 DATGEM010000000
VERSION     DATGEM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630847"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="VKQ27_05940"
     CDS             complement(<1..1720)
                     /locus_tag="VKQ27_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY88508.1"
                     /translation="MTLGFSGLLTRATIRSPLTPLFLMAALAAGLVALLTIPREEEPQ
                     ISVPMVDIVVQANGLKAPDAVELVTKPLEAIIKPISGVEHVYSQTQDDRVVVTARFDV
                     GTNEDDAVLRVNDKIRANLDKIPIGIPEPLIVGRGINDVAVVVLTLSPKPEAAARWTD
                     KDLYDLATKLRTELIKVDDIGTSYLVGTGAEQIRVEPDPERLSLFGVTLQQLIGKVRD
                     ANRSFVAGAVREAGGMRTLAAGQTLRGVPDIGLLLLTSRDNRPVYVRDVAKVVIGPST
                     AESRVWNLSPDRDGWQRVPAVSLALAKRAGANAVVVSQAIVDRVAALEGRLIPPDVRV
                     DVTRDYGKTANDKANELLFHLALATVSIVVLIALAIGWREAVVTLVVIPTTILLTLFA
                     ARLMGYTINRVSLFALIFAIGILVDDAIVVVENIARHWAMRDGRSRMQVAVEAVAEVG
                     NPTIVATLTVIAALLPMLFVSGLMGPYMAPIPANASAAMLFSFFVAMVVAPWLLLKLA
                     PSAAPAGDGHHDEGRLGRLYRTVAAVVLRSRVRAWTFLLGVGVATIAACVMFYTEAVT
                     VKLLPFD"
     gene            complement(1755..2738)
                     /locus_tag="VKQ27_05945"
     CDS             complement(1755..2738)
                     /locus_tag="VKQ27_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY88509.1"
                     /translation="MTAYRHIVVVAALLVAFTVAAVADSTFTVRRENLADQKAVFATV
                     ESPNVVPARARIGGTVASLSVKQGDLVKPGQVIAVVGDEKLLLQINSLDAQIAGLQSQ
                     LAQAQTDLTRAETLFRQGVGPRTTMDQARTATEVATAALRARNAERAVALQNLQEGQV
                     LAPVAGRVVTVPLTTGTVVLNGDTIATIGEQPFMLRLRVPESHATALKVGDAIRVDAG
                     QLGTGEGTAGKITLIYPQIEQGRVVADAQVDDLGNYFVGDRVRVWIDAGTREGFVVPA
                     QYVLTRFGLDYVRLRQSDGAVIETPVQRGALTAAGMEILSGVRNGDVLVAP"
     gene            complement(2735..2971)
                     /locus_tag="VKQ27_05950"
     CDS             complement(2735..2971)
                     /locus_tag="VKQ27_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLY88510.1"
                     /translation="MKDVKVLGSGCKRCVAAAAMIEAEARKLGVAVSLEKVTDAAAIA
                     SYGVMATPGVVIDGKLVHAGGLPRAEDIVRWLAA"
     gene            complement(2983..4029)
                     /locus_tag="VKQ27_05955"
     CDS             complement(2983..4029)
                     /locus_tag="VKQ27_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HLY88511.1"
                     /translation="MRSLSHSDGTKDASAILGVLVALVASLALYSQLEPFSRWVVDAL
                     AIPAGTRLHDAAAFFAYDTPKVLLLLALVVFGMGVVRSFFSPERTRRMLTGRGEGAGN
                     VLAASLGIVTPFCSCSAVPLFIGFVSAGVPLGVTFSFLIAAPMVNEVALGLLFGLVGW
                     RVALAYLVFGLGVAIVAGWVIGRMRLEHWLEPWVREVRQAPGGPDLGAITPRQRIQAG
                     VSAVQDIVGRIWHWVILGIAAGALIHGYVPTDMLATIMGKGSWWSVPAVVLIGVPMYS
                     NAAGIIPVVEALLGKGAALGTVLAFMMSVTALSFPEMIILRKVLSLRLIAVFIGVVAT
                     GILVVGFLFNALFA"
     gene            complement(4039..4398)
                     /locus_tag="VKQ27_05960"
     CDS             complement(4039..4398)
                     /locus_tag="VKQ27_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLY88512.1"
                     /translation="MNTKLQLHEMTPTEVHHALHRHEIVLIDVREPQEYVTERIHGAL
                     LFPLSSFDPRALPLGDRPVVLHCGSGKRSATAVAKCIEAGVPVTTHMKGGIMAWKHAR
                     HPTITLDPNTGAVIDRQ"
     gene            4767..>5740
                     /locus_tag="VKQ27_05965"
     CDS             4767..>5740
                     /locus_tag="VKQ27_05965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HLY88513.1"
                     /translation="MSEVVVLGAGMGGTLVTYELVPQLRPGDRLTLIGQDARYHFVPS
                     NPWIAVGWRERQDIEADLTGIMRKKGVRYLTQGARRVDPQAHRIELNDGESIAYDYLV
                     IATGPDLAFDEIEGLGPNGHTQSICHIDHAVKAHDAFEAFCKNSGPIVVGAVQGASCF
                     GPAYEFAYILDTELRKRRIRDRVKMTFVTPEPYIGHLGLDGVGDTKSLMESEFRNRHI
                     SWITNAKVQQVSPGMMSVEEVDDSGALRKTHELPFGFSMMLPAFRGIPAIRGLEKLVN
                     PRGFVLIDDHQQNPHFPEVFGIGVCVAIPPVGPTPVPVGTPKTGFMIES"
BASE COUNT         1129 a         1996 c         1666 g          949 t
ORIGIN      
        1 tgtcgaacgg cagcagcttc accgtcaccg cctcggtgta gaacatcacg caggcagcaa
       61 tggtggcgac gccgacacct agcaggaatg tccaggcgcg cacacgagaa cgcagcacca
      121 ccgcagcgac cgtacgatac agccggccca gccgcccctc gtcgtgatgc ccgtcacccg
      181 ccggcgctgc agacggcgcc agcttcagca gcagccacgg cgccaccacc atggcgacga
      241 agaacgagaa cagcatcgcc gccgacgcat tcgccggaat tggcgccatg tacggcccca
      301 tcagcccaga cacgaacagc atcggcaaca gcgccgcgat cacggtcagc gtcgcgacaa
      361 tggtaggatt gccgacctcc gccactgcct cgaccgccac ctgcatgcgg gacctgccgt
      421 cgcgcatcgc ccagtgacgc gcgatattct ccaccaccac gatggcgtca tccaccagga
      481 taccgatagc gaagatcagc gcgaacaggc tgacgcgatt gatcgtgtag cccatcagac
      541 gcgcggcaaa cagcgtcagc agaatggtgg tgggtatcac cacaagcgtt accacggcct
      601 cgcgccaccc gatggccagt gcgatcagca ccacgatcga caccgtggcg agcgccaggt
      661 ggaacagcag ttcgttcgcc ttgtcgttcg ccgtcttgcc atagtcacgt gtgacatcca
      721 cccgcacatc cggcgggatc agccggcctt ccagcgccgc gacacgatcg acaatagcct
      781 gcgacacaac aacggcgttc gcccctgccc gcttcgccag tgccaggctc accgccggca
      841 cgcgctgcca gccgtcgcga tcgggcgaca ggttccacac acggctctca gccgtgctgg
      901 gcccgatcac caccttggca acatcgcgca catagaccgg acggttgtca cggctggtca
      961 gcaacagcag gccgatatcc ggcactcccc gcagcgtctg acccgccgcc agggtgcgca
     1021 tcccgcccgc ctcgcgtacc gcgccggcaa cgaacgagcg gttggcgtca cgcaccttgc
     1081 caatcagctg ctgcaacgtc acgccgaaca gcgacagtct ttccggatcc ggttccaccc
     1141 tgatctgttc ggcgccggtg ccgacgagat aactcgtgcc aatgtcatca accttgatca
     1201 gttccgtccg caacttggtc gcgaggtcgt acagatcctt gtcggtccag cgtgccgccg
     1261 cttccggctt cggcgacagc gtcagcacca ccaccgccac gtcattgatg ccgcgtccaa
     1321 cgatgagcgg ctccgggatg ccgatgggta tcttatcgag attggcgcgg atcttgtcgt
     1381 tcacccgcag cactgcgtcg tcttcattgg tgccgacgtc gaaacgcgcc gtcaccacaa
     1441 cgcgatcatc ctgcgtctgg ctatagacat gctcgacgcc gctgatcggc ttgatgattg
     1501 cctcgagcgg cttggtcacc agctcgaccg catccggcgc cttcagccca ttcgcctgca
     1561 ccacgatatc gaccatcggc acgctaatct gcggttcttc ctcgcgcggt atggtcagca
     1621 gcgccaccag gcccgccgca agcgccgcca tcaggaacag cggtgtcaac ggcgaacgta
     1681 tcgtcgcgcg agttagcagg ccggaaaatc cgagggtcat acacagtttc gggcgaatgc
     1741 ccgaccttcc tcggtcatgg cgctaccagg acgtcgccgt tcctgacgcc ggagaggatc
     1801 tccatccccg cggccgtcag tgccccccgc tgcaccggtg tttcgatcac cgcaccatcc
     1861 gactggcgca acctgacata atccaggcca aagcgcgtca atacatactg ggctggcacc
     1921 acgaagccct cgcgtgtccc ggcatcgatc cacacacgga cccggtcgcc gacgaagtag
     1981 ttgccgagat catccacctg cgcatcggcc accacgcggc cttgttcgat ctgcggatag
     2041 atcagcgtga tcttcccggc agtcccctcg ccagtgccca gctgcccggc gtcaacccgg
     2101 atcgcatcgc cgactttgag cgctgtcgcg tggctctcgg ggacgcgcag acggagcatg
     2161 aacggctgct cgcctatggt ggcgatcgtg tcgccattca gcaccacggt accggtggtc
     2221 aacggcaccg tcaccacgcg gccggccacc ggcgccagca cctggccttc ttgcagattc
     2281 tgtagcgcca ccgcgcgttc ggcattacgt gcccgcagcg cagccgtcgc gacttccgtc
     2341 gcggtacgcg cctggtccat cgtggtgcgc ggcccgacgc cctgacggaa cagcgtttcc
     2401 gcccgcgtca ggtcggtctg cgcctgcgcc agttgcgatt gcagcccggc gatctgcgca
     2461 tcgagcgagt tgatctgcag cagcagcttc tcgtcgccga cgacagcaat aacctgtccc
     2521 ggcttcacga gatcgccttg cttcaccgaa aggctggcga ccgtgccgcc gatacgcgca
     2581 cgcgcaggca cgacattcgg actttccacc gtcgcgaaca ccgccttctg gtcagccaga
     2641 ttctcgcgcc tgaccgtgaa cgtgctgtca gcaactgccg ccacggtgaa cgcaacaagc
     2701 agtgccgcaa cgacgacaat gtgccggtaa gcagtcatgc ggcgagccag cgcactatgt
     2761 cttctgcacg cggcaacccg cccgcatgca caagcttgcc gtcgatcaca acgccaggcg
     2821 tcgccattac gccatagctg gcaatcgccg cggcatcggt gaccttctcc agcgacaccg
     2881 ccacgccgag tttcctggct tcagcttcga tcatcgcggc ggctgccaca cagcgtttgc
     2941 atccggagcc gagtactttg acgtccttca tggcacacct cgtcaggcaa acagcgcgtt
     3001 gaacaggaac ccgaccacca gaatcccggt cgctaccacg ccgatgaaca ccgcgatcag
     3061 ccgcaggctt agcaccttgc gcaggatgat catttcggga aacgaaagcg ccgtgaccga
     3121 catcatgaac gccagcaccg tgcccaaggc cgcgcctttg cccagcagtg cctcgacgac
     3181 cggaatgatc ccggcggcat tcgaatacat cggcacgcca atcagcacga cggccggcac
     3241 cgaccaccat gaccccttgc ccatgatcgt ggccagcata tcggttggca catagccatg
     3301 gatcagagcg ccggcagcaa tgccaaggat gacccagtgc cagatgcggc cgacgatgtc
     3361 ctgcaccgcg ctcacccccg cctggatacg ctgcctcggt gtgatggccc cgaggtcggg
     3421 tccgcccggc gcttgccgca cctcgcgaac ccacggttcc agccagtgct ccagccgcat
     3481 gcggccaatc acccagcccg cgacaatggc gacacccagg ccgaacacga gataagccag
     3541 cgccacacgc cagcccacca ggccgaacag caggccgagc gccacctcgt tcaccatcgg
     3601 cgccgcaatc aggaaggaga aggtgacgcc aagggggacg ccggctgaga caaagccgat
     3661 gaacagcggc accgcggagc acgagcagaa cggcgtgacg atcccaagcg atgcagcgag
     3721 cacattgccg gcgccctcgc cccggcctgt gagcatgcgg cgcgtgcgct ccggcgagaa
     3781 gaagcttcgc acaacgccca taccgaacac gaccagcgcc aacagaagca ggaccttcgg
     3841 cgtgtcatag gcaaagaacg cagcggcatc atgcaacctt gttcctgcgg gaatcgccag
     3901 ggcatccacc acccagcgcg agaatggctc gagctgactg tacagcgcca gcgaggcgac
     3961 gagagcgacc agcacaccaa gtatcgcact ggcgtccttg gttccgtcgc tgtggctcag
     4021 gctgcgcata gcatccgctt actggcgatc gatcactgcg ccggtgttcg gatcgagcgt
     4081 gatcgtggga tggcgcgcgt gcttccatgc catgatcccg cccttcatat gcgtggtcac
     4141 aggcacaccg gcctcgatgc acttcgccac cgcggtcgcc gaacgcttgc ctgacccgca
     4201 atgcagcacc accggccgat cgcccaaggg cagcgcccgc ggatcgaagc tggacaacgg
     4261 aaacagcagc gccccgtgta tccgctcggt gacatactcc tgcggctcgc gcacgtctat
     4321 cagcacgatc tcatggcgat gcaacgcgtg atgcacctct gtcggcgtca tctcatgcaa
     4381 ctggagcttt gtattcatct gcgtctcctg cattgaagat aatgatgcgc ccgacggcgg
     4441 cattgatttg ggtcaatgcg tccgcgtgcg gaccgcatta aggacaacgg tgacctgcgc
     4501 cgccgtacgg acggcgcgca catggagcat ggtttgcaga atatctcgaa cagggtttcg
     4561 agaacgcgcc gtgtcggcag gctggcattc gcataccaga tggtctgccc gcccagacac
     4621 gcgcacgagt ccgtcgtgcc gctgcagcga cagataccgc gataccgtcg gatcatgcag
     4681 ctcgaggaat tctgccagct caccgatgga acgctcattc ccgatcagtt ggcgcgggac
     4741 cctcaggcgt tgaatggaga gatctcatga gcgaagtcgt ggtgctgggc gcagggatgg
     4801 gcggcacgct ggttacctac gaactcgtgc cgcaactccg ccccggtgat cggctgaccc
     4861 tgatcggcca ggacgcacgc tatcatttcg tgccatccaa cccctggatc gccgtaggat
     4921 ggcgcgagcg tcaggacatc gaagccgatc tcaccggcat catgcggaag aagggcgtcc
     4981 gctacctgac gcaaggtgcg aggcgcgtcg atccgcaggc ccatcgcatc gagctgaacg
     5041 acggcgagag tatcgcctac gattatctgg tgatcgccac tgggcctgat ctcgcattcg
     5101 atgagatcga ggggctgggt ccgaacggcc atacgcagtc catctgccac attgatcacg
     5161 ccgtgaaggc ccacgacgcg ttcgaggcct tctgcaagaa ctccggcccc atcgtcgtcg
     5221 gcgcggtgca gggtgcctcc tgcttcggac cggcatacga gtttgcctac atcctcgata
     5281 ccgagctgcg caaacgccgg atacgcgatc gcgtcaagat gacgttcgtc acaccggaac
     5341 cctatatcgg ccatctcggc cttgatggcg tgggcgatac caaatcgctg atggaaagcg
     5401 agttccgcaa ccggcacatc agttggatca ccaacgccaa ggtgcagcag gtatcgcccg
     5461 gcatgatgtc ggtcgaggaa gtggacgaca gcggtgccct gcgcaagaca catgagctgc
     5521 cattcggctt ttcgatgatg ctgcctgcgt tccgcggcat tccagccatt cgcggcctgg
     5581 aaaaactggt gaatccgcgc gggttcgtgc tgatcgacga ccatcagcag aacccgcact
     5641 ttccggaggt gttcggcatc ggcgtttgcg tcgccattcc gccggtcggg ccgacgccgg
     5701 taccggtcgg cacgccgaag accggcttca tgatcgaatc
//
LOCUS       DATGEM010000186         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_292456, whole genome shotgun sequence.
ACCESSION   DATGEM010000186 DATGEM010000000
VERSION     DATGEM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292456"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..222
                     /locus_tag="VKQ27_05970"
                     /pseudo
     CDS             46..222
                     /locus_tag="VKQ27_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            364..>2888
                     /locus_tag="VKQ27_05975"
     CDS             364..>2888
                     /locus_tag="VKQ27_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HLY88514.1"
                     /translation="MRWLPDGQLDFMGRVDHQVKIRGYRIELGEIEATLRRNVAVGQV
                     AVVAREGAGGDKQLVGYVVQKQIPTQDDHERREAEHIGEWKAFYEQAYASGTDLASAE
                     DFGIWNSSYDGQPIALSHMREWRAAAVERILALKPKHVLEIGVGSGLMLWQVAPHCES
                     YWGTDFSEHTIQALRLRTLAQAELADKVQLRTQPAHRFDDLPEQYFDTIVINSVIQYF
                     PSAEYLVDVLRQALDRLVPGGRIFLGDVRNLRLLRCFATAIALQRHADLDAAQLQPIV
                     DKAMRLEKELLLDPDFFLALQEHVADIAGVDVQLKRGTLANELTRHRYEVVLYKRGSE
                     VTSLAHIPALDFGDDIADLAALEQRLAGARRALRVRAIPNGRIADEIAAMRRIWNLPS
                     HTGSALDPETCLRLGERLGYAVFASWSDEAAFDLVFDPDPSSRPVDIYQPTRSIKNGS
                     RYSNTPYAFKDTRAWVAQLRRALAEKLPDYMVPAALVVLEALPLTPNGKLDRKALPAP
                     DFTPASRRMPRTPQEEILADLFAEILSLDRIGIDDSFFDLGGHSLLATRLVSRIRSTL
                     DVELPIRTLFEAPTVAELAHHLDRGGRARMALRPMPRPARIPLSFAQQRLWFLNQLEG
                     PSATYNIPLALTLDGQLDVDALRQALGDLVARHESLRTIFTQTDGIAEQQILDPSAAT
                     LSFETAAVTEHDLPEALRRATARPFDLAHEIPLRAVLFTLAPTRAVLLLVAHHIAADG
                     ESLAPLLHDLAAAYTARCTGGEPAWAPLPVQYADYTVWQREWLGQESDAASPIAAQIA
                     YWKNALADLPEQLPLPTDRPRPAQGSHRGGHVSFDVGAGLHES"
BASE COUNT          478 a          992 c          927 g          491 t
ORIGIN      
        1 gtatggaccg gcggcgaagc cgccgcgccc gctgcgttcc aggcggtgct cgaccgctgc
       61 cccgacctcg ccctggtgca cgtctacggc ccgaccgaga cgaccacctt cgccacccac
      121 tacgacctgc gcgcgccgca tcgcgtcggc gccagcgtgc cgatcggcgc accgatggac
      181 aacacgcaag tgcacgtgct cgatcacgcg ctgcagccgg taccggttgg tgtgtgcggt
      241 gaactctata ttggcggcag cgggctggcg cgcgggtatc tgcagcggcc ggggctgacc
      301 gcagagcgtt tcgtcgccga cccgttcggt actggcgcac gtctgtatcg caccggcgat
      361 ttggtgcgct ggctgccgga cggccagctc gacttcatgg gtcgcgtcga ccatcaagtc
      421 aaaattcgcg gttaccgtat cgagctcggc gaaatcgagg cgacgctgcg gcgcaacgtc
      481 gcggtggggc aggtggccgt cgtcgcgcgc gagggcgctg gcggcgacaa gcagctggtc
      541 ggctatgtgg tgcagaaaca gatccccacg caggacgacc acgaacgccg tgaagccgaa
      601 cacatcggcg aatggaaagc gttctacgag caggcgtacg ccagcggtac cgatctggca
      661 agtgccgaag acttcggtat ctggaacagc agctacgacg gccagccgat tgcgctttca
      721 catatgcgcg aatggcgtgc ggcggcggtg gagcggattt tggcgctcaa gccgaagcac
      781 gtgctggaaa tcggcgtcgg ctcgggcttg atgctgtggc aggtcgcgcc gcactgcgag
      841 tcgtattggg gcacggactt ctccgaacac acgatccagg ccttgcgcct gcgcacgctt
      901 gcgcaggcgg aactggccga caaggtgcag ctgcgcacgc agccggcgca tcgtttcgac
      961 gacctgcccg agcagtactt cgacacaatc gtgatcaact cggtgatcca gtatttcccc
     1021 agtgcggagt atctggtcga cgtgctgcgc caggcgctcg atcgcctggt tccgggtgga
     1081 cgcattttcc tcggcgatgt gcgcaatctg cgcttgctgc gctgctttgc cacggcgatc
     1141 gccctgcaac ggcacgcgga cctcgatgcc gcacaattgc agccgatcgt cgacaaggcg
     1201 atgcgtctgg agaaggagct gctgctcgat ccggatttct tcctcgcact gcaggaacac
     1261 gttgccgaca tcgccggcgt cgacgtgcag ctcaagcgcg gcacgctggc gaacgagctg
     1321 acgcggcatc gttacgaggt ggtgctgtac aagcgcggca gcgaggtcac gtcgctagcg
     1381 cacattccgg cgctcgattt cggcgacgat atcgccgatc tggcggcgct cgaacagcgt
     1441 cttgccggcg cacgccgcgc cttgcgcgtg cgcgccattc cgaacggacg catcgccgat
     1501 gaaatcgctg cgatgcgccg gatctggaac ctgccgtcgc acaccggttc ggcgctcgat
     1561 ccggaaacct gcctgcgtct gggtgaacgg ctcggctatg ccgtgttcgc gtcctggtcg
     1621 gacgaggccg cgttcgacct ggtgttcgat ccggacccgt cgagccgacc ggtcgacatc
     1681 tatcagccga cgcgatcgat caagaacggg tcgcgctaca gcaatacccc ctatgccttc
     1741 aaggacacgc gcgcctgggt tgcgcaactg cgccgcgcct tggccgagaa gctgcccgac
     1801 tacatggtcc cggccgcact cgtcgtgctc gaagcgctgc cgctgacgcc gaacggcaaa
     1861 ctcgaccgca aggccctgcc tgcgccagac ttcacgccgg cgagccgccg catgccgcgt
     1921 acgccgcagg aggaaatcct cgccgacttg ttcgccgaaa tcctgagcct ggatcgcatc
     1981 ggcatcgacg attccttctt cgatctgggc ggacactcgt tgctcgccac gcgcctggtc
     2041 agccgcattc gttccacgct cgatgtcgag ctgccgatcc gaacgctgtt cgaagcaccc
     2101 accgtcgccg aactggcgca ccatctggac cgcggcggcc gcgcgcgcat ggccttgcgc
     2161 ccgatgccgc gtccggcgcg catcccgttg tcgttcgcgc agcagcgctt gtggttcctc
     2221 aaccagctcg aaggaccgag cgcgacgtac aacatcccgc tcgcgctgac gctcgacgga
     2281 caactcgacg tcgacgcgct gcgccaggcg ctcggcgatc ttgtcgcgcg ccacgaaagc
     2341 ctgcgcacga tcttcacgca gaccgacggt atcgccgagc agcagatcct cgatccgtcc
     2401 gctgcgacgc tgagcttcga aaccgcggcg gtcaccgaac acgacctgcc cgaagccctg
     2461 cgccgtgcca cggcgcgtcc gttcgatctc gcacatgaaa tcccgctgcg cgctgtgctc
     2521 ttcacgctcg cgccgacacg cgctgtgttg ctgctcgtcg cgcaccatat cgccgcggac
     2581 ggcgaatcgc tggcgccgct gctgcacgat ctcgccgccg cttataccgc gcgctgcacc
     2641 ggcggcgaac ccgcgtgggc gccgttgccg gtgcaatacg ccgattacac cgtgtggcag
     2701 cgcgaatggc tcggccagga atccgatgcc gcgagtccta tcgcggcaca gatcgcgtac
     2761 tggaaaaacg cgctcgccga tttgccggaa cagctgccgc tgccgaccga tcggccgcgg
     2821 ccggcgcaag gcagccaccg aggcggccat gtgtcattcg acgtcggcgc ggggttgcat
     2881 gaatcctt
//
LOCUS       DATGEM010000187        11635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1547726, whole genome shotgun sequence.
ACCESSION   DATGEM010000187 DATGEM010000000
VERSION     DATGEM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547726"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VKQ27_05980"
     CDS             <1..296
                     /locus_tag="VKQ27_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY88515.1"
                     /translation="TGTFTFVLHKGGGPSRPLLKQPKAETPTHFISMGLNEDLDQAMK
                     QALREMISFICSRSNLSREQAYQFCSLAVDFHVTQTVNGEKGIHGMLKKGLLF"
     gene            296..1603
                     /locus_tag="VKQ27_05985"
     CDS             296..1603
                     /locus_tag="VKQ27_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY88516.1"
                     /translation="MQPCQLTAAEAATLIRAKKLSCEELVRSCLARIAARDADVKAWL
                     WLDPDHVIRRARELDKLPPQGPLHGLPFGVKDIIDTIDYPTTQNSPIYDGARIGRDAA
                     CVAVVRGAGALILGKTDTVEFASGGRKALTRNPYNFAHTPGGSSSGSGAAVGDFQVPL
                     AFGTQTGGSHIRPASFNGIYGIKPTWNLLSREGVRMSSITLDTVGWYGRSIDDLILVG
                     TAYRLPVDTAPVAVKGLRVGLCRSPMWHAIEPAGATALETAAKRLLDAGAIVEELVLP
                     SPFDRLPEAQNTIVTAEGRVSFLPEYVNAHALLAPDLKAKVESGITREELLAAYTVAD
                     TCRPVFDGMFGSSLDVVLTPSAPGEAPEGLHTTGNAIFNRMWTLLHVPCVGIPACRGS
                     NNLPVGVTLVGPRLTDARLLAISKALAPIIDSDPQVGLRDLCG"
     gene            1651..2679
                     /locus_tag="VKQ27_05990"
     CDS             1651..2679
                     /locus_tag="VKQ27_05990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HLY88517.1"
                     /translation="MILTGDVNLMNIDEPLVPFARVHDEFRAADIVFSNLECCLHEPP
                     RGHAVENEGFFASPEIGGKALRAAGIAAVGLANNVNYGEAAISASIARLDELGIAHTG
                     AGANADAAHAPAIVTRNGVRYGFLQRSSVYWPTNHEAGAGSTGIAVVRAHTAYQVPMH
                     KTRPEIPPMNRPGIPPIIVTWTDPDYLRRFTEDVAALRSQADIVVASLHWGLHKEVFA
                     YMRELAHAAIDGGADIVIGHGPHYSLPVEAYRGRPIFYGLGSFSFHTGHGGRKHGDWI
                     GMMVRAAVAGKAIIGTSFQFVRHNDQNETVPCRLAEEAAEFADIKTRSESLGTKLIPR
                     MDEVRIAL"
     gene            2713..4155
                     /locus_tag="VKQ27_05995"
     CDS             2713..4155
                     /locus_tag="VKQ27_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88518.1"
                     /translation="MDPAPEPRRASPRTVSLIVASAMFMEQLDGTVLATALPSMARSF
                     HADPLHMNVALTCYLLTLAMFIPASGRVADRFGSRTVFRAAIALFTLGSVGCAQAPTL
                     SFLVAARMLQGVGGAMMSPVGRLVMLRAVSKSELVNAMAWLMIPATIGPILGPPVGGF
                     IVTYLSWHWIFYINVPIGIAGIILVTRYIEEMREPTRTRFDLRGLALSGTALTSFMFG
                     IEMASRGVGSVNVALGLVGAGVISASLYMWHARRCPQPMLDFRMMQIVTFRMSVICGS
                     LSRIAVGAMPFLLPMMLQLGFGLSAAQSGLITFVTSIGSLTMRLCAPRILRRLGFRNV
                     LVWIGLAATLLLAASAAFRPSWPVIFIYVVLLLNGFFQSLQFMAYNTIAYADVPRPQM
                     STATSFYTTFQQMSLTLGIAISAAVLAASEHLLGHAQLALSDFSVAFLAVAAISLLAP
                     LLSSRLDKSAGAELSGHRDRPAKPRLAAAE"
     gene            complement(4270..5454)
                     /locus_tag="VKQ27_06000"
     CDS             complement(4270..5454)
                     /locus_tag="VKQ27_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY88519.1"
                     /translation="MPADDAAFSGAWGNGAWGGIAPTALIVEQVDGDGTVQLIYARGS
                     TEQPKIAAHWLRVTGRIEDHRLTIHLPDPDSSAGFLVHYRIMAPGQLEGDLTTRDGWR
                     THAFLQQIAGPPSAIIATAALPFRPIWREIRILEHSTVGDASGQTIQLAATLYRSRLP
                     GRQPLVILNHGAPGTVGVERTWHFEAEARFFLMNGFNVVVPMRKGRGESGGPYLERTT
                     IPTAIQVESAVEDEDTVVNAMRAETWVDPARIIVAGWSRGGGLSVVYAGRHPDKVAGV
                     INFSGGWWSEYEGKYEPETSGVGEHQMRELLAAAGKTTRVPELWLYADNDMYFSIAYA
                     RQIFDAFHADGGTGDFLAFGKVSGVPSSTEGNGHALFEHVGMWEGAVAAYLRQIEKAP
                     VH"
     gene            complement(5656..6096)
                     /locus_tag="VKQ27_06005"
     CDS             complement(5656..6096)
                     /locus_tag="VKQ27_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY88520.1"
                     /translation="MIRPAHADEADAVRDVVHEAYRHYIARIGKPPGPMLDDYAQRIA
                     DGQTWVLEHAGGIAGILVLEETADGLLLDNIAVAPEQQGKGVGRALLEFAEAEARRRE
                     FTAIHLYTHELMTENLLLYARIGYVETYRITEKGFDRVYMRKPL"
     gene            complement(6093..7598)
                     /locus_tag="VKQ27_06010"
     CDS             complement(6093..7598)
                     /locus_tag="VKQ27_06010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HLY88521.1"
                     /translation="MPYTPPADAPRCAVVDLGSNSVRLVVYEGNCRNPVGIFNEKAVL
                     RLGRGLQATGRLNDEGMAQAFTVLHRYYAVAQAMGAAPFEILATAAVRDARNGPDFVA
                     GLQARIPDVPIRVLSGVQEAEYSAMGVLCGIPTADGILADIGGGSLEMVRLDRGTRGV
                     SQTLSLGAIRLAERANGDPARARAIAEADLATVPWLIEGANRDLYLVGGAWRALARIH
                     MSQTGYPLQMVHHYTISREEARDLTGVIAGAGRRALERLPGLSRRRIDDLPFAAVALR
                     RVLRATGVRRVVFSASGLREGWFMQRMPEAIRAEDPLLAAGKELARRLGRDPSLPPAL
                     MVWTDPLFPGETGEAHRLREAACWMSDIGSHDHPEFRAEQAFLRVLRQPGIGLDHHAR
                     AFLALAIAMRYEVDGDADFLRPARLLLDVAATHRAEMLGVSLRLAYTLSAGTTDLLAG
                     TELRLAGGRLILRLQEDSGVFAGESVMRRLDRLAQAVGLEAATEAAREQAA"
     gene            complement(7603..9792)
                     /locus_tag="VKQ27_06015"
     CDS             complement(7603..9792)
                     /locus_tag="VKQ27_06015"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HLY88522.1"
                     /translation="MVAELDRPASTAGDAMIGLDSPDRFINRELSWLDFNHRVVEEAE
                     NPRHPLLERVRFASISASNLDEFYSVRVAGLIGQAKAGITAISPDGRTPAQQLAEIKL
                     RAESLLAEQQRVWRELRGLLRGAGIEVCEPESLSADDLKWLDHWFMERVFPVLTPLAI
                     DPAHPFPFIPNMGLVMALLLLREEDGQTMRGLLPIPSQVDRFVRLPGATGEAIRFVLL
                     DDLITMFLDRMFPGFRVTGQGTFRLIRDTDVEFEEEAEDLVRSYETALKRRRRGVAIH
                     LSTDASMPEELRALVAAELGAAPDETHVANGILGVVDLKQLIVDDRPDLLFPPYTPRF
                     PERIRDFGGDCFAAIRAKDIIVHHPFESFDVVVQFLRQAARDPNVIAVKQTLYRTSRD
                     SPIVNALIEAAEAGKFVTAVVELRARFDEEANIRMSREMEAAGVQVVFGFAELKTHAK
                     LSLVVRREGAGVRSYAHFGTGNYHPITARIYTDLSFFTTDPDLTRDSARLFNYLTGYA
                     RPNKMDEIAFSPLTMRATLTELIHKEIAFATAGKPATIWLKMNSLVDETLIDLLYEAS
                     RAGVKVMGVVRGICCLRPGVPGLSENIRIKSIIGRFLEHGRILAFGNGHALPSRNARV
                     YISSADWMVRNMDWRVETLVPIHNATVHAQVLDQIMVTNLKDSMQSWELHSDGSWERV
                     PRAKKPVSAHDYFMTNPSLSGRGSALHGTAVASPLANHRRQDRVQQD"
     gene            complement(9848..10570)
                     /locus_tag="VKQ27_06020"
     CDS             complement(9848..10570)
                     /locus_tag="VKQ27_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HLY88523.1"
                     /translation="MSPYHQPALPIFDEPRYSAANFVAAASNSEALAWLSRGGEWPGG
                     RLALWGGAGCGKTHLLHLWAELTGAAFWRGADLREFPDLPANGIALDDADAADERALF
                     HLLNAAAEADLPLLIASRSAPGRWPVQLPDLRSRLRAITAVEVGPPDDSLLRALLPRL
                     LAQRLIRLPEPVMIWLLNRLPRTVPLLREAVARLDAVALEQRREITVPMARDVLAGLL
                     SADGNEISGTEAAPSPDERRLL"
     gene            complement(10623..10880)
                     /locus_tag="VKQ27_06025"
     CDS             complement(10623..10880)
                     /locus_tag="VKQ27_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88524.1"
                     /translation="MTDDELLEAYHTALHSYACAKSGHGNRVEAFVRLTSAQMALIGR
                     FGANDYLWRYCQQYEVDTDALGDSGIARERQVSSDLPTNVG"
     gene            11343..>11635
                     /locus_tag="VKQ27_06030"
     CDS             11343..>11635
                     /locus_tag="VKQ27_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HLY88525.1"
                     /translation="MFRGDLSTARGYWLAWIDSLLVDHAVFRLVWSNFHAVAPGRLYR
                     SNHPTPARLAALTRRYGLKTLINLRGRTGNGSDALSRAAAEELGLEFVDMPLE"
BASE COUNT         2091 a         3834 c         3632 g         2078 t
ORIGIN      
        1 tcaccggcac gttcaccttc gtgctgcaca agggcggcgg gccgtcgcgt cctctgctga
       61 agcagccaaa ggccgagaca ccgacgcatt tcatcagcat gggcctcaac gaagacctgg
      121 accaggcgat gaaacaggcg ctgcgcgaga tgatctcgtt catctgctcg cgcagcaatc
      181 tgtcgcgcga gcaggcgtat cagttctgct cgctggcggt ggatttccac gtcactcaga
      241 ccgtgaatgg cgagaaaggc atccacggta tgctgaagaa ggggctgctg ttctgatgca
      301 gccctgccag ctcaccgcgg cggaggccgc cactctgatc cgcgcgaaaa agctttcctg
      361 cgaggagttg gtgcggtcct gcctggcgcg catcgcggca cgtgatgccg acgtgaaggc
      421 ctggctgtgg ctggaccccg atcacgtgat ccgccgcgca cgcgaactgg acaagctgcc
      481 gccgcaaggg ccgctgcatg ggctgccgtt cggcgtgaag gacatcatcg atacgatcga
      541 ctacccgacc acgcagaact cgccgatcta cgacggcgcg cggatcggac gggatgcagc
      601 ctgcgtggcc gtggtgcgcg gagccggcgc actgattctg ggcaagaccg acacagtcga
      661 gtttgcctct ggcggccgca aggcgctgac gcgcaatcca tacaacttcg cacatacgcc
      721 cggcggatcg tcgtcggggt ccggcgcggc ggtgggcgac ttccaggtgc ctctggcgtt
      781 tggcacgcag accggtggct cgcatatacg gccagcctcg ttcaacggca tctatggaat
      841 caagccgacg tggaatctgc tgagccgtga aggcgtgcgg atgtcgtcca tcacgctcga
      901 tacggtggga tggtatggtc gttccataga tgatctgatc ctggtcggaa cggcataccg
      961 gctgccggtg gatacggcgc ctgtcgcggt gaagggcctc cgggtggggc tctgccgcag
     1021 cccaatgtgg cacgcgatcg aaccggccgg cgcaacggcg ctggagacag cggcgaagcg
     1081 gctgctggac gcgggggcga tcgtcgagga gctggtgctg ccgtcgccgt tcgaccggct
     1141 gccggaggcg cagaacacga tcgttacggc agaaggccgt gtttcgttcc tgccggaata
     1201 cgtcaacgca catgcgctgc tggcgccaga cctgaaggcg aaggtcgaga gcgggatcac
     1261 gcgggaggaa ctgctcgcgg cctacacagt tgccgatacc tgccgccctg tattcgacgg
     1321 gatgttcggt tcgtccctgg acgtggtcct gacgccgagc gcgccgggcg aggcgcccga
     1381 agggctgcac accacaggta acgcgatctt taaccgcatg tggacgctcc tccatgtgcc
     1441 gtgcgttggc atccctgcgt gccgtgggtc gaataatctg ccggttggcg tgacattggt
     1501 gggaccgcgg ctcacggatg cccggttgct ggcgatctcc aaggcgctgg ctccgatcat
     1561 cgactcggac ccacaggttg gattgcgcga tttgtgcgga tagagtccgc tgccagcgcc
     1621 cgccacgaag gcagggcttc ggggggcacg atgatcctga ctggcgatgt caacctgatg
     1681 aacattgacg aaccgctcgt tccgttcgcg cgcgtgcacg acgagttccg ggcggccgat
     1741 atcgtattca gcaacctgga gtgctgcctt cacgagccgc ctcgtggcca tgcggtcgaa
     1801 aacgaaggct tcttcgcgtc acccgaaatc ggcggcaagg ccctgcgcgc agccggcatc
     1861 gctgcggtcg gccttgccaa caacgtgaat tacggcgagg cggcaatcag cgcttccatc
     1921 gcgaggctcg acgagctcgg tatcgcccac accggtgccg gcgcaaatgc cgacgcagca
     1981 cacgccccgg ctatcgttac gcggaatggc gtccgctacg gcttcctgca acgaagctcg
     2041 gtctactggc cgacgaacca cgaggccggg gcagggtcca cgggtatcgc tgtggttcgt
     2101 gcgcacaccg cctatcaggt gccgatgcac aagacacggc ccgagattcc accgatgaac
     2161 cggcccggta tcccgccgat catcgtcacc tggaccgatc ccgattatct ccggcgcttc
     2221 accgaagacg ttgcagcact ccgaagccag gcggacatcg ttgtcgcatc cctgcattgg
     2281 ggcctgcaca aggaagtgtt cgcctatatg cgcgagcttg cccatgctgc gatcgatggc
     2341 ggtgccgata tcgtgatcgg gcatggaccg cactactcgc tgcccgtcga ggcatatcgc
     2401 ggccggccga tcttttacgg gctgggcagc ttctcgttcc acaccggtca cggcggccgc
     2461 aagcatggtg actggatcgg catgatggtg cgcgctgccg tcgccggaaa ggcgatcatc
     2521 ggcaccagct ttcagttcgt gcgccacaac gatcagaacg aaaccgttcc gtgccgcctc
     2581 gccgaagaag cagcggaatt cgccgatatc aaaacgcgca gcgaatcgct cggcacgaaa
     2641 ctgattccac gcatggatga ggtgcgtatc gcgttgtagt cgctcgcccg gcatccggta
     2701 tcatcgccgc ccatggatcc cgcccccgag cctcgccgcg ccagcccacg caccgtctcc
     2761 ctgatcgtcg caagtgccat gttcatggag cagctcgacg gcacggtcct ggccacggcg
     2821 ctcccctcga tggcgcgaag tttccacgcc gatccgctgc acatgaacgt ggcgctcacg
     2881 tgctatctgc tgacactggc gatgttcatc cccgccagcg gcagggtcgc ggatcgtttc
     2941 gggtcacgta ccgtgttccg cgcggcgatc gcgttgttca ctctgggttc ggtgggctgc
     3001 gcccaggcgc cgacactctc ctttctggtg gcggcgcgca tgttgcaagg cgtgggcggc
     3061 gcaatgatgt cgcccgtggg gcggctggtg atgctgcgcg ccgtgtcgaa gtcggaactg
     3121 gtcaacgcca tggcctggct gatgattccg gcgaccatcg ggccaatcct cgggccgccg
     3181 gtgggcgggt tcatcgtcac ctatctgtcg tggcactgga tcttctatat caacgtgcct
     3241 atcggcattg ccgggatcat cctcgttacg cgctatatcg aagaaatgcg tgagccgacg
     3301 cgcacgcggt tcgacttgcg cggcctggcg ttgtcgggga ccgcgctcac atccttcatg
     3361 ttcgggatcg agatggccag ccgtggtgtc ggctcggtga atgtagcctt ggggctggtc
     3421 ggcgccggcg tgatctccgc gagcctctat atgtggcacg cgcgtcgctg cccccagccg
     3481 atgctcgatt ttcgcatgat gcagatcgtc acgttccgca tgtccgtgat ctgcggctca
     3541 ctatcgcgta ttgcagtcgg cgcgatgccg ttcctgctgc cgatgatgct gcaactcggc
     3601 ttcgggctga gcgcggcaca aagcgggttg atcacgttcg tgacgtcaat cggatcgttg
     3661 acgatgcggc tctgtgcacc aaggatcctg cggcggctgg ggttccgcaa tgttctcgtc
     3721 tggatcggcc tggcggcaac gctgctgctc gctgccagcg cggcgttccg gcccagttgg
     3781 ccggtgatat tcatctacgt ggtgctgctg ctgaatgggt tcttccagtc gttgcagttc
     3841 atggcctaca acacgatcgc ttacgccgat gtgccccggc ctcagatgag cacggcgacg
     3901 agcttctata ctacgttcca gcagatgtcg ctgacactgg ggattgcgat ctcggcagcg
     3961 gtactcgccg cttctgaaca tctgctcggg catgcgcagt tggcgctttc cgacttctca
     4021 gtggcgttcc tggcggtggc agcgatttcg ctgttggcac cgcttttaag cagccggctg
     4081 gacaagtcgg caggcgcaga actcagtggg catcgcgatc ggcctgccaa gccgaggctg
     4141 gctgcggcgg agtgatccaa acgaggcaac ccccgcgatg accttccagt cactgtcatt
     4201 gcgagcgaag cgaagcaatc ccgcgcgaga ttgcttcgcg tcactcgcag gtgtcagccc
     4261 gatatggctc taatgcactg gcgccttttc gatctgccgc agataagctg ccacagcgcc
     4321 ctcccacatg ccgacatgtt caaacaatgc gtgaccattg ccctcggtgc tgctcggcac
     4381 gccggaaacc ttcccgaagg ccaaaaaatc gccggtcccg ccatcagcat ggaaggcatc
     4441 gaagatctgc cgcgcgtaag caatcgagaa gtacatgtcg ttgtccgcat aaagccagag
     4501 ctctggcaca cgcgtggttt tccccgcggc agctagcagc tcccgcatct gatgttcccc
     4561 aacacccgat gtttccggtt catacttgcc ctcatactcg ctccaccagc caccggagaa
     4621 attgatgacg ccggccacct tgtcggggtg ccgccccgca taaacgacag acagaccgcc
     4681 accgcgtgac caccccgcga caatgatacg cgccggatcg acccaagttt cggcacgcat
     4741 ggcattgacg accgtatcct catcttcgac agcggactcg acctgaattg ctgttggaat
     4801 ggtggtccgc tcgaggtagg gacctccgga ctcgccgcgc cccttccgca tcggcaccac
     4861 aacattaaat ccgttcatca ggaaaaaccg ggcctcggct tcaaaatgcc aggtcctctc
     4921 caccccgacg gtccccggcg ctccgtggtt aaggatcacc agcggctgcc gcccgggcag
     4981 ccgcgagcga tacagtgtcg ccgcaagctg aattgtctga cccgatgcat cgccgaccgt
     5041 tgaatgctcc aggatacgga tctcacgcca gatgggtcgg aatggcagtg cggcagtggc
     5101 gattatagcc gacggcggcc cggcgatttg ctgaaggaag gcatgcgtac gccagccgtc
     5161 ccttgtggta aggtcgccct ccaactggcc cggcgccatg atccgataat ggaccagaaa
     5221 gcccgctgac gagtcggggt ccggtagatg gatcgtcagc cgatgatctt cgatacgtcc
     5281 agtcacgcgc agccaatggg ctgcgatttt cggctgttcg gtgctgcccc tggcataaat
     5341 caactggaca gtcccatccc catcgacctg ctcgacgatg agagcggtcg gtgcgatgcc
     5401 accccatgca ccgttgcccc acgcgcccga gaacgctgcg tcgtccgcgg gcacggttgc
     5461 agcaggtgtc tgtattgaca gatcagttgg caatggaacc tgcgcacacg ccagactgca
     5521 taccgcggac agcatgacac tggcgagagc aatgcggagc ctcatggcgc aggccttcca
     5581 cgatcgaacg cagtcgacag gccgtttgtt atatccggca cttcgtcggc tggcaccaca
     5641 agagcgcgag aggcgctaca acggcttccg catatacact cggtcgaagc ccttctccgt
     5701 gatccgatac gtctcgacat acccgatgcg ggcgtacaac aagaggttct cggtcatcag
     5761 ctcgtgtgta tacaaatgga tggccgtgaa ttcgcggcgc cgcgcctcgg cctcggcgaa
     5821 ttccagcagc gcgcgcccca cccctttgcc ctgctgctcc ggcgccacgg cgatgttgtc
     5881 cagcaacaat ccgtcggccg tctcctccag caccaggatg ccggcgatgc cgcccgcgtg
     5941 ctccagcacc caggtctgcc cgtccgcgat acgttgcgcg tagtcgtcca gcatcgggcc
     6001 gggcggcttg ccgatacgcg cgatgtaatg gcggtatgcc tcgtgcacca catcccgcac
     6061 cgcgtcggct tcgtcggcat gcgccgggcg gatcatgccg cctgctcgcg cgccgcttcc
     6121 gtcgcggcct ccaggccaac cgcctgcgcc agccgatcga ggcgacgcat cacgctctca
     6181 ccggcgaaca ccccgctatc ctcctgcaac cgcaggatca gccgaccgcc ggccaggcgc
     6241 agttcggtgc cggcgagcag atcagtggtg ccagcggaca gcgtataggc aagccgcaac
     6301 gacaccccca gcatttccgc ccggtgcgtc gcggcaacat ccagcaatag ccgcgccggg
     6361 cgcaggaagt cggcatctcc gtccacctcg taccgcatcg cgatagccag cgcgaggaaa
     6421 gcgcgggcat ggtggtcaag accgatgcca ggctggcgca gcacgcgcag aaaggcctgc
     6481 tcggcacgga attcggggtg gtcatggcta ccgatgtccg acatccagca ggccgcctca
     6541 cgcagacggt gcgcttcgcc tgtttcgccg gggaacagtg ggtcggtcca caccatcagc
     6601 gctggcggca ggctgggatc gcggccgagc cggcgggcca gttccttccc ggcggccagc
     6661 agaggatcct cggcgcgtat cgcctccggc atgcgctgca tgaaccagcc ttcgcgcagg
     6721 ccactggcac tgaacaccac gcgacgcacg ccagtcgcgc gcagcacgcg gcgtaacgcc
     6781 acggcggcga acggcaaatc atcgatgcgg cggcgcgaaa gaccgggcag gcgttccaat
     6841 gcgcgccggc ccgcgccggc gatcacgccg gtcaggtcac gcgcctcctc gcggctgatc
     6901 gtgtagtgat gcaccatctg cagcgggtag ccggtctgcg acatgtggat ccgcgccagc
     6961 gcgcgccacg caccgccgac gagataaagg tcgcggttgg cgccctcgat cagccagggg
     7021 accgtggcga gatcggcttc ggcaatcgcg cgggcgcggg cggggtcgcc attggcgcgt
     7081 tcggcgaggc ggatcgcgcc caggctcagg gtctgcgaca cgccgcgcgt gccgcgatcc
     7141 aggcgcacca tctccagcga accgccgccg atatcggcca ggatgccatc cgcggtcggg
     7201 atgccgcaca gcacgcccat ggccgagtat tccgcctcct ggacgccgga cagcacgcgg
     7261 atcggcacgt ccggtatccg cgcctgcaac ccagccacga aatccggccc gtttctcgca
     7321 tcgcgcaccg cggccgttgc caggatctca aacggcgcgg cacccatcgc ctgcgccacc
     7381 gcatagtacc ggtgcagcac ggtgaacgcc tgggccatgc cctcgtcatt cagccgtccg
     7441 gtggcttgca ggccacggcc aagccgcagc accgccttct cgttgaatat cccaacggga
     7501 ttgcggcagt tgccctcgta caccacgagg cgcaccgagt tcgacccaag atccaccact
     7561 gcgcagcgcg gcgcatcggc gggtggcgta tacggcattc gatcaatcct gctgtacgcg
     7621 gtcctgacga cgatggttgg caagcggcga tgcgaccgcg gttccgtgca acgcggagcc
     7681 gcgcccagag agtgacggat tggtcatgaa gtagtcgtga gcggagaccg gctttttcgc
     7741 ccgcggcacg cgctcccatg agccgtcgct gtgcagctcc cacgactgca tcgagtcctt
     7801 caggttggtc accatgatct ggtccagcac ctgcgcatgc actgtcgcat tgtgaatcgg
     7861 caccagggtt tcgacgcgcc agtccatgtt gcgcaccatc cagtccgcgg agctgatgta
     7921 cacacgcgcg ttccggctcg gcaacgcgtg gccattgccg aacgccagga tacggccgtg
     7981 ctccaggaac cgcccgataa tggacttgat gcggatgttc tccgacaggc ccggcacgcc
     8041 agggcgcaga cagcagatgc cgcgaaccac gcccatcacc ttcacgccgg cgcgggacgc
     8101 ttcgtagagc agatcgatca aggtctcatc gaccagcgag ttcatcttca gccagatcgt
     8161 ggccggcttg ccggccgtgg cgaacgcgat ctccttgtgg atgagttccg tcagcgtcgc
     8221 gcgcatcgtc agcggactga aggcgatctc gtccatcttg ttcggccgcg catagccggt
     8281 gaggtagttg aacagccgcg ccgaatcgcg cgtgaggtcc gggtcggtgg tgaagaacga
     8341 caggtccgtg tagatacgcg cggtgatcgg atggtagttc ccggtgccga aatgcgcgta
     8401 cgaacgtaca ccggcgcctt cccgccggac caccagcgac agtttcgcgt gcgtcttcaa
     8461 ttccgcaaag ccgaatacga cctggacacc agccgcttcc atctcccgcg acatacggat
     8521 gttggcttcc tcgtcgaacc gcgcgcgcag ttccaccacg gcggtgacga acttgccggc
     8581 ctcagccgcc tctatcaggg cgttgacgat cggggagtcg cggctggtgc gatacagcgt
     8641 ctgctttacc gctataacgt tgggatcgcg cgcggcctga cggaggaact gcaccaccac
     8701 gtcgaaactt tcgaacgggt ggtggacgat gatgtccttg gcacggatcg cggcgaagca
     8761 atcgccgccg aaatcgcgga tgcgctcggg gaagcgcggc gtgtagggcg ggaacagcag
     8821 gtccggccga tcgtccacga tcaactgctt cagatcgaca acgcccagga taccgttggc
     8881 gacatgcgtc tcatccggcg cggcaccgag ttcggcggca accagggcac gcaactcctc
     8941 cggcatgctg gcgtccgtac tcagatggat cgccacgccg cggcgccggc gcttcaaggc
     9001 ggtttcatac gaacggacaa ggtcttcggc ctcctcctcg aattccacat cggtgtcgcg
     9061 gatcaggcgg aatgtgccct gcccggtcac gcggaagccc ggaaacatcc ggtcgaggaa
     9121 catcgtgatc agatcgtcca gcaggacgaa gcggatcgcc tcgccggtcg cgccaggcag
     9181 ccgcacgaaa cgatcgacct ggctaggtat cggcagcagg ccgcgcatcg tctggccgtc
     9241 ttcctcgcgc agcagcaaca gggccatcac caggcccatg ttgggaatga acgggaacgg
     9301 atgcgccgga tcgatcgcca gcggcgtcag gaccggaaag acccgctcca tgaaccagtg
     9361 gtcgagccat ttcagatcgt cggcggaaag cgactcgggc tcgcagacct caatgccggc
     9421 gccacgcagc aggccccgca actcgcgcca tacgcgctgc tgctccgcca gcagtgactc
     9481 ggcacggagc ttgatttcgg caagctgctg cgccggtgtc cggccatcag gggagatcgc
     9541 ggttataccc gccttcgcct ggccgatcag gcctgcaacg cgcaccgaat agaattcgtc
     9601 caggttggac gcgctgatgg aggcgaagcg aacgcgctcc agcaggggat gccgcgggtt
     9661 ttccgcttct tcgacgaccc gatggttgaa gtcgagccag gacagttcgc ggttgatgaa
     9721 acgatccgga ctgtccaggc cgatcatcgc gtcgccggct gtcgacgcag gacgatcaag
     9781 ttccgcaacc atgccatcat tgctgggcgc cataaccgct ccatctgatg tgctcacggc
     9841 gccgatccta caggaggcgc cgctcgtccg gcgagggggc cgcttctgtc ccggaaattt
     9901 cattcccatc cgcagagagt aggccggcca gcacgtcgcg ggccatgggg acggtgatct
     9961 cgcgacgctg ttcaagcgcc accgcgtcca gcctcgccac tgcctcgcgc agcagcggca
    10021 ccgttcgtgg caggcggttc agcagccaga tcatgaccgg ctctggcaac cggatcagac
    10081 gctgagccag caatcgcggc agaagggcgc gcagcagtga atcgtccggt gggcccactt
    10141 ccacagcggt gatcgcgcgg agccggctcc gcaggtcggg caactgcacc ggccatcgcc
    10201 ccggcgccga acggctggca atcagcaacg gcagatcagc ttccgcggcc gcgttcagca
    10261 aatggaacag cgccctttca tcggctgcat cggcgtcatc cagcgcaatc ccattcgctg
    10321 gcagatcggg gaattcgcgc aggtctgcgc cacgccagaa tgctgcgccg gtcagttccg
    10381 cccagaggtg cagcaggtgc gtcttgccgc aacccgcacc accccacagc gccagccgcc
    10441 cgccaggcca ctcgccgccg cggctcagcc aagcgagcgc ctccgagttg gacgccgccg
    10501 caacaaagtt cgcagccgaa tagcgcggct cgtcgaagat cggcagcgcg ggctggtgat
    10561 aaggggacac aggcgcaatc ggcacccgcc ccccggttcc gtcaagccat ttgctctcag
    10621 cgtcagccca cgttcgttgg cagatccgat gatacctgcc gctccctcgc tatgcccgag
    10681 tcaccgagag catccgtatc gacctcatac tgttggcagt agcgccatag atagtcgttg
    10741 gcgccgaacc ggccgatcaa cgccatctgt gctgaagtca gcctgacaaa cgcctcgacc
    10801 cgattgccat gccccgattt ggcacatgcg taactgtgca gggcagtgtg gtaggcctcc
    10861 agcagttcgt catcggtcac gaccaacctc catcacgagt gccggcgcag gcgatactgt
    10921 cgcgatggca tcgcatacgc cgtcgggcac ttggtcacgc tgccatggga ggccatgaag
    10981 tcggccacca ttttttcaat ctgggcggga cttagtccga gacgcgcttc gcaacgacaa
    11041 cgctgcttga cgtgctgtgc ttgtaaggcc tcctcagaca gccgggtcgg atcgcgtgat
    11101 gtcttggtat catcaaaggt cgtgtgcatg agcccacccc cttttggaaa atctccgcat
    11161 ttcgacgcaa ttctgcgagc ctaaagttat cgttgccttt atgaagtgct ggtcacagcc
    11221 acgcaaagat gcccgggtcc gccatgcgac cagagcagga cacgcgctcc agttgcgctt
    11281 tgatcattgt catgcccata tgcaagtgac ttgcaagttg catataggga tcaatggaca
    11341 gcattttccg aggcgatctc agcaccgccc ggggctattg gcttgcgtgg atcgattcac
    11401 tccttgtcga tcacgcggtg ttccgcctcg tttggtccaa cttccatgcc gtcgcgccgg
    11461 gccggcttta ccgatccaat caccccacgc ccgcgcgcct cgccgcgctg acacgccgct
    11521 acggcctgaa gacgctgatc aacctgcgtg gccggaccgg caacggttcg gacgcactga
    11581 gccgggcggc ggcggaagag ctggggctcg aatttgtcga catgccgttg gagag
//
LOCUS       DATGEM010000188         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_213477, whole genome shotgun sequence.
ACCESSION   DATGEM010000188 DATGEM010000000
VERSION     DATGEM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213477"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VKQ27_06035"
     CDS             <1..772
                     /locus_tag="VKQ27_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88526.1"
                     /translation="GLSVHMAKIIECASLCCLPGGRDSILATLDDDGFVLESMAEQRR
                     ATPVSVAAHSLYEQADPFTVYEPEGRLDLSAADYAAVDERRCRVTGATWHASTEPCVK
                     IEGARKLGERAVLLAGAADPRFIAQHRDILPAVATVVRDLVCEDAPEDYTLRFRVYGV
                     DGVRMAAPPGEPNPGEVFVMGECIAPTAERAAEVVRTLKQYLLHHGYAGRLSTAGNLA
                     FPFTPPEVSLGPAYRFNVYHLLRTPDPDGLFPLEVETL"
     gene            complement(775..1941)
                     /locus_tag="VKQ27_06040"
     CDS             complement(775..1941)
                     /locus_tag="VKQ27_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88527.1"
                     /translation="MRKLLVTALVAALISPAMAADTVRGVTNDEILIGTYTDLSGVTA
                     MWGVNNSNAWRMVFEETNAAGGINGRKIKYILEDNQYQVPRSVQAANKLINRDGVFVM
                     VADGGTPMNNATMPMQLAAGIPNVFPLTSARSMYEPLNHLKFGLASSYYDQIRSGVKL
                     FVEQHGKKTVCAMSEDTDFGRDVMDGVRDQLKAQNMKLAAETLHKPTDTDFSASVARL
                     HDANCDLIVVGGIVRDTVQIISAVRKTGWKVDMLGQAASYDEAVAEVPGGVTEGFYSM
                     APVLFVANSSENAAVVKFAQDYKKEFGKEPNFAAQIGYTGAQLVVQALKNAGKDLTAD
                     SFVSGMESIKDWHDIFGSPPMTFSATKHQGSNESFLCVVKDGKWVPVSMTPVGY"
     gene            complement(1957..>2142)
                     /locus_tag="VKQ27_06045"
     CDS             complement(1957..>2142)
                     /locus_tag="VKQ27_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88528.1"
                     /translation="RRELGLRRLRRAYVGAAPLSADIERWAAALGLNIQQIDGQATKG
                     IAVDPRYRALMEEAYGT"
BASE COUNT          375 a          731 c          623 g          413 t
ORIGIN      
        1 gggcctgtcg gtgcacatgg caaagatcat cgaatgcgcc tcgctgtgtt gcctgccagg
       61 cgggcgcgat tcgatcctcg ccacgctcga tgatgatggc ttcgtgctgg aaagcatggc
      121 cgagcaacgc cgtgccacgc cggtttcggt cgccgcgcac agcctttacg agcaggccga
      181 tccgttcacg gtgtacgaac cggaaggccg gctcgatctc agcgccgccg attatgctgc
      241 ggtagacgaa cggcgctgcc gcgtcaccgg cgccacatgg cacgcatcaa ccgagccatg
      301 cgtgaagatc gaaggcgccc gcaagctcgg cgaacgagcc gtgctgctgg ccggcgccgc
      361 ggacccacgc ttcatcgccc agcatcgcga catcctgccg gccgtagcga cagtcgtgcg
      421 cgacctggtc tgcgaggatg cgccggaaga ctacacgctg cgcttccgcg tctatggcgt
      481 ggatggcgtg cgcatggcgg caccacctgg cgaaccaaac ccaggcgagg tcttcgtcat
      541 gggtgagtgc atcgcgccaa cggccgaacg cgcggccgaa gtggtgcgca ccctcaagca
      601 atatctgttg catcacggct atgcggggcg gctctcgacc gccggcaatc tcgccttccc
      661 gttcacaccg cccgaggttt ccctgggccc ggcgtaccgg ttcaacgtct accacctcct
      721 gcggacaccg gacccggatg ggctgtttcc gctggaggtg gagactctct gacctcagta
      781 gccgaccggc gtcatgctga ccggcaccca cttgccgtcc ttcaccacgc acaggaacga
      841 ctcgttcgat ccctgatgct tggtcgcgct gaaggtcatc ggcggcgagc cgaagatatc
      901 gtgccagtcc ttgatgctct ccatgcccga gacgaagctg tccgccgtca gatccttgcc
      961 ggcattcttc agcgcctgca ccacgagctg cgcaccggtg tagccgatct gtgcggcaaa
     1021 gttcggctcc ttgccaaatt ccttcttgta gtcctgagcg aattttacca ccgcggcgtt
     1081 ctcgctgctg ttggcgacga acagcactgg cgccatcgaa tagaagccct cggtcacgcc
     1141 gcccggcact tcggcgacgg cctcatcata gctcgcggcc tgaccgagca tgtcgacctt
     1201 ccacccggtc ttccgcaccg ccgagatgat ctgcaccgta tcgcgcacga tgccgcccac
     1261 aactatcaga tcgcagttcg catcatgcag cctcgccaca gacgcgctga aatccgtgtc
     1321 cgtcggcttg tgcagcgtct ccgccgcgag cttcatgttc tgcgccttca gctggtcacg
     1381 cacgccatcc atgacgtcgc ggccgaaatc ggtatcctcc gacatcgcgc acaccgtctt
     1441 cttgccgtgt tgctcgacga acagcttcac gccagagcgt atctggtcgt agtacgatga
     1501 agccagaccg aacttcagat ggttcagcgg ctcgtacatc gagcgcgccg aagtcagcgg
     1561 aaacacgttg ggaatgcccg cagccaactg catcggcatc gtcgcgttgt tcatcggcgt
     1621 gccgccatcg gcaaccatga cgaagacgcc atcgcgattg atcagcttgt tggccgcctg
     1681 gaccgagcgc ggcacctgat actgattatc ctcgaggatg tacttgatct tgcgcccatt
     1741 gataccgccg gccgcgttcg tctcctcgaa caccatgcgc caggcattgg agttgttgac
     1801 gccccacatc gcggtgacgc cggaaagatc agtgtaagtg ccaatcagga tttcgtcatt
     1861 ggtcacgccg cgcacagtgt ccgcggccat ggccggcgat atcagtgcag cgaccagcgc
     1921 cgtcaccaat agcttacgca tcgctcgttc cttccctcaa gtgccgtagg cttcttccat
     1981 cagggcgcgg tagcgcgggt ccacggcgat gccctttgtc gcctggccgt cgatctgctg
     2041 tatgttcagg cccagcgccg ctgcccatct ctcgatgtcg gcggaaagtg gcgctgctcc
     2101 gacataggca cgacgcaacc ggcgcagccc cagttcccgt cg
//
LOCUS       DATGEM010000189         9423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1316170, whole genome shotgun sequence.
ACCESSION   DATGEM010000189 DATGEM010000000
VERSION     DATGEM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316170"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VKQ27_06050"
     CDS             complement(<1..367)
                     /locus_tag="VKQ27_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88529.1"
                     /translation="MDFKGKVALVTGAGNGIGRAAALGFANGGAKVVAVDRDTAAGEG
                     TAGIIRQQGGEALFVAADVTKSADVQAYVKAALDAYGSIDCFHNNAGIEGTIATTAEY
                     DEAMFDTVMAVNVKGVFLGL"
     gene            complement(414..905)
                     /locus_tag="VKQ27_06055"
     CDS             complement(414..905)
                     /locus_tag="VKQ27_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HLY88530.1"
                     /translation="MAERSDPLAELAFASPDCQAAQLTALPPARRHVVHGDAASLGMA
                     LPDSCRVIVDRDRVVLWLGPDEFLLFASDCDSPGAIDISHRDTAIEVSGPRAAWVINA
                     FCALDLDVSAFPVGMCTRTVFGKAQILLWRTAADTFRIEVARSFAPYVWGCLEEARRE
                     FLD"
     gene            complement(898..3738)
                     /locus_tag="VKQ27_06060"
     CDS             complement(898..3738)
                     /locus_tag="VKQ27_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HLY88531.1"
                     /translation="MTGVFRTEGGGRVDRTQPLEFRFDGRRMVGCQGDTLASALLANG
                     VRVVGRSFKYHRPRGIVAAGSEEPNALVTVDRGGGRVTPNLRATQVELYEGLRAVSQN
                     RFPSLVCDFGAVAGAFGPLLSAGFYYKTFMWPRGFWRHVYEPTIRAAAGLGRAPRGAD
                     PDRYLHQYAHCDTLVIGAGPAGLAAALAASAHGGRVMLCDEQAELGGSLLAEMEHAGW
                     VGRALSTLRQHAEVTLLPRTTAFGWYPDNMIGLVERITDHIADPDPRLPRERLWHVRA
                     KRVVIATGALERPLVFAGNDRPGVMLAGAARTYLQRYGVKVGARVVVLTVDDSAYAAG
                     VALREAGASVVVADTRTDPGDAARACGLPVQNGVFATGTHGRMRVDRVALSNGESLSC
                     DVVLMCGGWTPAVHLFSQSRGRLGFDEQLQAFLPGEWTERVRSVGACNGTFGTEACME
                     EGFAAGGAGAWLRAGEVLSGKYPASSSKIAFVDFQNDVTAKDLQLATREGFRSIEHVK
                     RYTTTGMATDQGKTSNMNALAAVSGELGRPVEDVGHTTFRPPYTPVTFGAFAGASRGD
                     LFDPVRQTPLHAWAVARGAVFENVGTWQRARYFPRGGEDMHQAVARECRAVRNAAGIF
                     DGSTLGKIEVTGPDAGEFLSRMYIHGFNRLAPGRCRYSMMLSEAGYVMDDGVVARLAA
                     DRFHVTTTTGGAASVLHHLEDYRQTEFPDLRVWLTSTTEQWAVIAVQGPRAHQVIAPL
                     VEGVDLATMPHMSMREARICGVPVRLFRVSFTGEVGYEVNVPADHGRALWDAVYAAGG
                     AHGITPYGTEAMHVLRAEKGYIIVGQETDGTVIPLDLGIASGQRRDFVGKRSLGRADM
                     LRVDRKQLVGLLAPEVLEEGAQVVGDGPSLGHITSSYWSEALGRPIALALVGGGRARI
                     GRVMHVPTGDRTIAVRVTDPVFYDKAGERLHG"
     gene            complement(3735..4007)
                     /locus_tag="VKQ27_06065"
     CDS             complement(3735..4007)
                     /locus_tag="VKQ27_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HLY88532.1"
                     /translation="MLLIACPWCGVRPENEFRYAGEAHIARPVDPSVVDDAAWAEFLY
                     MRANPKGRHAERWRHIHGCGRFFNCVRDTVSDRVLVTYRPGELPPS"
     gene            complement(4007..5275)
                     /locus_tag="VKQ27_06070"
     CDS             complement(4007..5275)
                     /locus_tag="VKQ27_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008365064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HLY88533.1"
                     /translation="MTAGGGALRFSIYSVLRHAVTGAAWPEQWRSPEPKAAYDVVIVG
                     AGGHGLATAYYLAREHGVRNVAVLERGWLGGGNTGRNTTIIRSNYLWDASAALYEHAL
                     KLWEGLAQELNYNVMYSPRGVMMLAHSVHDVQVFKRHVHANRVQGIDNEWLTPEEARA
                     FCPPLNISEELRYQVLGAALQRRGGVARHDAVAWGYARAASALGVDIIQNCEVTGFGR
                     GPSGAVNAVETTRGRIAARKVGVVAAGHTSVVMAMAGVRMPLESYPLQALVSEPVKPI
                     LPCVVMSNSIHAYISQSDKGELVIGAGTDAYTSYSQAGGLHIAEHTLEAICELFPTFR
                     RLRMLRNWGGIVDVTPDRSPIIGLTPVPGLFVNCGWGTGGFKATPGSGHVFAATVARG
                     EPHPIAAPFSLERFRNGALIDEAAAAAVAH"
     gene            5329..6942
                     /locus_tag="VKQ27_06075"
     CDS             5329..6942
                     /locus_tag="VKQ27_06075"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HLY88534.1"
                     /translation="MADFDYIIIGAGSAGCVLAHRLTEDLRTRVLLLEAGPKDDSMWI
                     NIPVGFTKLLNSPRFNWNFATEPEDNVKGRSIPIPRGKTLGGSSSINGMLYVRGNPLD
                     YNTWAQFGNRGWSYDSVLPYFRKSEHFEPGGDSTRGQGGLLNVANMVERAELLDAFID
                     AAAAEGWPRNPDYNNGKQEGFGYYQVTQKNGRRWSTARAFLDPARSRPNLRIETEAFT
                     TRILLEGKRAVGVEYRQNGQTHQERCNAEVILAAGAVKSPHILELSGIGQPELLQSLG
                     IEVRHELRGVGENYRDHFAPRMNWRVKLPVTLNEHTRGTRFVKEIVKYYTQGRGILTF
                     TAGIVYGFVKTRPELEEPDVQFHFAHASYATAQTRILDREPGMTLTVYQCSPESKGSI
                     HTKSSDPTAAPAIRPNFLSEELDRRTILQGMKIGRRIINNKVLDKYRAYEMNPGDKVQ
                     TDDEWLDFARMNGQTTYHVTGACKMGHDPMAVVDDELRVHGIDGLRVIDASIMPTLVS
                     GNTNAAVIMIAEKGADMIKAAAQPVRAAA"
     gene            complement(7120..8004)
                     /locus_tag="VKQ27_06080"
     CDS             complement(7120..8004)
                     /locus_tag="VKQ27_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLY88535.1"
                     /translation="MKILNVSHRTEYKYAQPVTFGAHRMMMRPRDSHDLRLLGTALTI
                     SPAASVRWLHDVFGNSVAIAEFSEPASELVVESSFQAEHYPLTEEGVTLEEFARHYPF
                     SYDASEIPDLVRTAERQYPDPDHRVDAWARRFVEQQRDGDTMAILMAMAHAIKDEFKY
                     NSRDEMGTQDPLVTLDGGSGTCRDFALFMMEAARSLGFAARFVSGYLYDERKMEGDEP
                     VVGVGATHAWVQIYLPGAGWVEIDPTNDLVGGRNLLRVAVTRDPKQAIPLSGTFTGAP
                     TDYLGLSVAVEVTTATAG"
     gene            complement(8045..8932)
                     /locus_tag="VKQ27_06085"
     CDS             complement(8045..8932)
                     /locus_tag="VKQ27_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HLY88536.1"
                     /translation="MIYRVRHTTRYDYRSPADLGVHLAHLRPRHLPVQAVLHSEVRAA
                     PAVSWRHEGRDHFGNDVTWLFLDAAHAAFEVTAEATVDVLFPGLPAAGATPAWEVVAR
                     RAAMGGAEGWEAAEFAFDSPLAPIDPEARAYAALSFLPGRPALAALLELNARIKRDFA
                     FRPGVTTTATPIRDVLRRREGVCQDFAHMMISMVRAFGVPARYVSGYIRTRPAEGQPR
                     RLGADQSHAWIGAWLGPEHGWVDLDPTNGIVVRDEHVVLAWGRDYSDVTPLRGVILGG
                     GEQALSVEVHLEALTDANA"
     gene            complement(8929..>9423)
                     /locus_tag="VKQ27_06090"
     CDS             complement(8929..>9423)
                     /locus_tag="VKQ27_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HLY88537.1"
                     /translation="SIADVVACTLEFPGAAEQSARVEHGLRLALELCDSTITYRSRYL
                     AVVQPAPVLDLVLADEGNPRGLAFQLATTRMLLTEIAGQTDQSLVATTDGLLGDVVAM
                     VQRVTQAADQAKAAVLLPDELRSMRDAVAELSDRLSRRYFAVLPTLRSVGLDAPARTM
                     RGAA"
BASE COUNT         1858 a         3619 c         2514 g         1432 t
ORIGIN      
        1 gcagcccaag aaacacgccc ttcacattga ccgccatcac cgtgtcgaac atcgcctcgt
       61 cgtattcggc tgtcgtcgcg atggtgccct cgataccggc gttattgtgg aagcaatcga
      121 tcgagccata agcatcgagc gccgccttca cataggcctg tacatcggcc gatttagtca
      181 catcggctgc gacgaacaaa gcctcgccac cctgttgccg gatgatgccc gcggtgcctt
      241 cacctgccgc ggtatcgcga tccacggcga cgaccttggc cccaccattc gcgaagccca
      301 acgcagcggc gcgcccgatg ccattgcccg cgccggtgac cagcgccact ttgcctttga
      361 aatccatggt ttcctccaat cggccaattg cgcgcagcct agcacgttgc gtatcagtca
      421 aggaactcgc gccgcgcttc ctccagacac ccccagacat aaggcgcgaa agaccgcgcc
      481 acttcgatcc ggaacgtgtc cgccgcggtg cgccacaaca ggatctgcgc cttgccgaac
      541 accgtgcgcg tgcacatgcc aaccggaaac gccgacacat ccaggtcgag cgcgcagaat
      601 gcgttgatca cccaggcagc acgtggaccg gagacctcga tcgcggtgtc gcggtgcgat
      661 atgtcgattg cgccaggact gtcgcagtcg gacgcgaaca gcaggaattc gtcggggccc
      721 agccatagca ccacgcgatc gcgatcgacg atgacgcggc agctatcggg cagagccatg
      781 ccgaggctcg cagcatcgcc gtgaacgaca tgtcgtcttg ccggtggcag cgcggtcagc
      841 tgcgccgcct ggcaatcggg cgaagcaaag gcgagctcgg ccagcggatc cgagcgctca
      901 gccatgcagt cgctcgcctg ccttgtcata gaacactgga tcggtcaccc gcaccgcgat
      961 cgtgcgatca cccgtcggca cgtgcatcac ccgcccgatc cgcgcgcgac cgccccccac
     1021 caatgccaac gcaatcggcc gtcccagcgc ctcgctccag tacgaagacg tgatgtgccc
     1081 cagcgacggc ccatcaccga caacctgcgc gccttcctcc aggacctccg gcgccagcaa
     1141 tccaacaagc tgtttgcgat cgacgcgcag catatcggcg cgtccaagcg atcgcttgcc
     1201 gacgaagtcg cgcctctgac cggacgcaat gccaagatcc aaggggatga ccgtgccatc
     1261 ggtctcctgc ccgacaatga tatagccctt ctcggcgcgc agcacgtgca tcgcctccgt
     1321 gccataaggc gtaatgccgt gcgctccacc ggccgcatac accgcatccc acaaggcgcg
     1381 accatgatcg gccggtacgt tgacctcgta gccaacctcg ccggtgaagc tgacgcggaa
     1441 caaccgcacc ggtacaccgc agatccgggc ctcccgcatg ctcatatgcg gcatcgtggc
     1501 aaggtccaca ccctccacca gtggtgcgat cacctggtgt gcgcgggggc cctgcaccgc
     1561 gatcaccgcc cattgctccg ttgtcgaagt cagccagacg cgaagatcag ggaactcggt
     1621 ctgccggtaa tcctccaggt gatgcagcac actcgcggcg ccgcccgtgg tcgtcgtgac
     1681 gtggaaccga tccgctgcca gtcgcgccac aacgccgtcg tccatcacat agccggcctc
     1741 gctcagcatc atgctgtatc gacagcgccc tggtgccagc cgattgaagc cgtggatata
     1801 catccggctc aggaactcgc ccgcatccgg tccggtcacc tcgatcttgc ccagcgtcga
     1861 gccgtcgaag atgccggccg cattgcgcac cgcgcgacat tcgcgcgcca cggcctgatg
     1921 catgtcttcg ccaccgcgcg gaaagtagcg tgcgcgttgc caggtgccga cgttttcgaa
     1981 cacggcacca cgcgcgaccg cccaggcgtg gagcggcgtc tgacgcaccg gatcgaacag
     2041 atcgccgcgc gacgcaccag cgaacgcacc aaatgtcaca ggtgtatatg gcggacggaa
     2101 cgtggtgtgc cctacatcct cgaccggtcg gcccaattcg ccggaaactg ccgccagcgc
     2161 attcatgttg gacgtcttgc cctgatcggt tgccatacca gtcgtggtat agcgcttcac
     2221 gtgctcgatc gaccggaagc cttcgcgcgt cgcgagttgc aggtccttcg cagtcacatc
     2281 gttctggaaa tctacgaacg cgatcttgct gctggaagcg ggatactttc cagacagcac
     2341 ctcaccagca cgcagccaag caccggcacc accagcagcg aacccttcct ccatgcacgc
     2401 ctcggttcca aacgtcccgt tgcacgcacc aaccgaacgc acgcgttcag tccattcgcc
     2461 cggcaggaac gcctgcagtt gctcgtcaaa acccagcctg ccacgcgatt gcgagaacag
     2521 atgcaccgcg ggagtccagc cgccgcacat cagcaccaca tcgcacgaca gactctcgcc
     2581 attggacagc gccacacgat caacccgcat ccgtccatgt gtcccagtcg cgaacacgcc
     2641 attctgcacg ggcaaaccgc acgcacgggc cgcatcgccc gggtccgtcc gtgtgtccgc
     2701 cacaacaact gatgctcctg cctctcgcag cgccactccc gccgcatagg cactgtcatc
     2761 gactgtcaga acgacaacgc gtgcaccaac cttcacgcca tatcgctgta aatacgtccg
     2821 cgctgcaccg gcgagcataa cgcccggccg atcattgcct gcgaacacca acggccgttc
     2881 cagcgcgccg gtcgcgatca ccacccgctt cgcgcgcacg tgccaaagcc gttcgcgcgg
     2941 cagccgagga tcagggtcag cgatatgatc cgtaatgcgc tcgaccagtc cgatcatgtt
     3001 gtcgggatac cagccgaacg ctgtcgtgcg cggcaacagc gtcacctcgg cgtgctgcct
     3061 cagcgtggat agcgctcggc ccacccatcc agcgtgctcc atctcggcca gcagcgaccc
     3121 gccgagttcc gcctgctcgt cgcacagcat cacgcggccc ccgtgtgccg acgccgccag
     3181 cgccgcagcc aatcccgccg gtcccgcgcc gattaccagc gtgtcgcaat gtgcatactg
     3241 gtgcagatac cggtccggat ccgccccacg cggcgcccgc cccagccccg cggcggcacg
     3301 gatcgtcggc tcatagacat gccgccagaa cccgcgcggc cacatgaacg tcttgtagta
     3361 gaatcccgcc gacagcagcg gcccgaatgc ccccgccacc gcgccgaaat cgcagaccag
     3421 cgacggaaac cggttctgac tgaccgctcg caacccctca taaagctcta cctgagtcgc
     3481 gcgcaaattg ggcgtcacgc gcccaccacc ccggtctacc gtgaccagcg cgttcggctc
     3541 ttccgacccc gcggccacga tcccccgagg ccgatggtac ttgaacgacc gccccaccac
     3601 ccgcacgcca ttcgccaaca gcgccgaggc cagcgtatca ccctggcatc caaccatccg
     3661 ccgcccgtcg aaccggaact cgagcggctg agtgcgatcc acgcgaccgc cgccttctgt
     3721 ccgaaatacc cccgtcatga tggcggcaac tcacccggcc ggtacgtgac cagcacccga
     3781 tcgctcactg tatcgcgcac gcagttgaag aaccgcccgc acccatggat gtgccgccat
     3841 cgctcggcat ggcgcccctt cggattggcg cgcatataca ggaactccgc ccacgccgcg
     3901 tcatccacta ccgacggatc aacgggccgg gctatatgcg cctcgccggc ataccggaac
     3961 tcgttctccg gccgcacccc gcaccacgga caagcaatca gcagcatcag tgcgccaccg
     4021 ccgctgccgc cgcctcatcg atcagcgccc cattccggaa ccgctccagc gaaaacggcg
     4081 ccgcgatcgg atgcggctcc ccccgtgcga ccgtcgccgc gaacacatgc cccgagccag
     4141 gcgtcgcctt gaacccgcct gtcccccagc cacagttcac gaacagccca ggcaccggcg
     4201 tcagcccgat aatcggcgag cgatccggcg tcacatcgac aatcccgccc cagttccgca
     4261 gcatccgcag ccgccggaac gtcggaaaca gctcgcagat cgcctctagc gtatgctccg
     4321 cgatatgcag cccccccgcc tggctgtacg acgtatatgc gtccgtccct gcaccgatca
     4381 ccagctcgcc cttgtccgac tgcgagatat acgcatggat gctgttcgac atcaccacgc
     4441 aaggcaggat cggcttcacc ggctccgaca ccagcgcctg caacggatag ctctccagcg
     4501 gcatccggac gccggccatc gccatcacca cgctggtgtg ccccgccgcc accacaccca
     4561 ccttgcgcgc cgcgatccgc ccgcgtgtcg tctccaccgc gttaaccgca ccagacggcc
     4621 cgcgcccaaa cccggtaact tcgcagttct ggataatatc cacgcccaga gcactagccg
     4681 cgcgcgcata cccccacgcc accgcatcat gccgcgccac gccgccgcgc cgctgcaacg
     4741 ctgcccccag aacctgatac cgcagctcct ccgaaatatt cagcggcgga cagaacgccc
     4801 gagcctcttc gggcgtcagc cactcattgt cgataccctg cacccggttg gcatgcacat
     4861 gccgcttgaa cacctgtaca tcgtgcaccg aatgcgccag catcatcacg ccccgcggcg
     4921 agtacatcac gttgtagttc agctcctgcg ccaacccctc ccacagcttc agcgcatgct
     4981 cgtacagcgc cgcgctcgca tcccacaaat agttggaccg gatgatcgtg gtgttgcggc
     5041 cggtattgcc gccacccagc cacccgcgct ccagcactgc gacattccgc acgccatgct
     5101 cgcgcgccag gtagtaggcg gtcgccaagc catggccacc ggcgccgaca atcaccacat
     5161 cgtacgcggc cttaggctcc ggcgagcgcc actgctccgg ccatgccgcc cccgtcaccg
     5221 catgccgcaa tacgctgtag atcgaaaacc gcaacgcccc accgcccgcc gtcattgcga
     5281 ggagcatagc gacgaagcaa tctagagggg agagtggagg aaacggatat ggctgacttc
     5341 gactatatca tcatcggcgc cggttcggcc ggctgcgtcc tggcccaccg cctgaccgag
     5401 gacctgcgca ccagggtgct gttgctcgaa gccggcccga aggacgattc gatgtggatc
     5461 aacatccccg tcggcttcac caagctgctc aacagcccgc gcttcaactg gaacttcgcc
     5521 accgagccgg aagacaacgt caaaggccgc tcgatcccca tcccccgcgg caagaccctg
     5581 ggcggttcaa gctcgatcaa cggcatgctc tacgtgcgcg gcaacccgct cgactacaac
     5641 acctgggcgc aattcggaaa ccgaggctgg tcctacgatt cggtcctccc ctacttccgc
     5701 aaatccgaac acttcgaacc gggcggcgac tcaacccgag gccagggcgg cctgctcaat
     5761 gtcgccaaca tggtcgagcg cgccgaactg ctcgacgcct tcatcgatgc agcagctgca
     5821 gaaggctggc cgcgcaaccc cgactacaac aacggcaagc aggaaggctt cggctactat
     5881 caggtcacgc agaagaacgg ccgccgctgg tccaccgcgc gcgccttcct cgatcccgcc
     5941 cgcagccgcc ccaacctgcg catcgagacg gaagccttca ccacgcgcat cctgctggaa
     6001 ggcaagcgcg ccgtcggtgt ggagtatcgc cagaacggcc agacccacca ggagcgctgc
     6061 aacgccgagg tgatcctcgc cgccggcgcg gtaaaatctc cgcacatcct ggaactctca
     6121 ggcatcggcc agccggaact gctgcaatcg ctcggcatcg aggtccgcca cgagctgcgc
     6181 ggcgtcggcg aaaactaccg cgatcacttc gcgccgcgca tgaactggcg cgtgaagctc
     6241 cctgtcaccc tgaacgaaca cacgcgcggc acgcgattcg tgaaagagat cgtcaagtac
     6301 tacacgcaag gccgcggcat cctgaccttc accgccggca tcgtctatgg ctttgtcaag
     6361 acgcgtcccg agctggaaga gcccgacgtc cagttccact tcgcccacgc cagctacgcc
     6421 accgcgcaga cgcgtatcct ggaccgcgaa cccggtatga cactcaccgt ctaccagtgc
     6481 agcccggaat cgaaaggctc gatccatacc aagtcatccg accccacggc cgctccggcg
     6541 atccgcccca acttcctctc cgaggaactc gatcgccgca ccattctgca gggcatgaag
     6601 atcggccgcc gcatcatcaa caacaaggtg ctggacaagt accgcgccta tgagatgaac
     6661 cccggcgaca aagtccagac cgacgacgaa tggctcgatt tcgcgcgcat gaatggccag
     6721 accacctacc acgtcaccgg cgcctgcaag atgggccacg acccgatggc agtcgttgat
     6781 gacgaactcc gcgtgcacgg catcgacggc ctccgcgtca tcgacgcctc gatcatgccg
     6841 acgctggtct ccggcaacac caatgccgcg gtcatcatga tcgcggaaaa aggcgccgac
     6901 atgatcaaag ccgccgccca gccggtccgc gccgccgcgt agctcaacca ccccaaggcg
     6961 cggcccacgg ccacccaccg tcacccgtcg cccacggtcg cccgtcgccc accgtcatgg
     7021 ccggccttga gccggccatc gctctccgca tgtacactgg gtgcgtcagg aagctgatat
     7081 cctgcccaac gggccggcca tgacaacgga gccgcgcaat caacccgcag tagccgtcgt
     7141 aacctccacc gccacgctca gcccaagata atccgtcggc gccccggtga acgtcccgct
     7201 cagcgggatc gcctgcttcg gatcccgcgt caccgccaca cgcagcaggt tccgcccacc
     7261 caccagatcg ttggtcggat cgatctccac ccaccccgcg ccaggcagat agatctgcac
     7321 ccaggcatgc gtggcgccca caccaacaac cggttcgtct ccctccatct tccgctcgtc
     7381 atacagatat ccagacacga accgagccgc gaacccgagc gaccgcgccg cctccatcat
     7441 gaacagcgca aaatcccggc acgtcccact gccaccatcc agcgtaacga gtggatcctg
     7501 cgtccccatc tcatctcgcg aattgtattt gaactcatct ttgatcgcgt gcgccatggc
     7561 catcaggatc gccatcgtat caccgtcgcg ctgctgctcg acaaagcgcc gcgcccatgc
     7621 atccacgcgg tgatccggat cgggatactg tcgctccgcc gtccgcacca ggtcgggaat
     7681 ctccgatgcg tcataactga acggataatg ccgcgcgaat tcctccagcg tcacaccttc
     7741 ttcggtcaga ggataatgtt cggcctgaaa cgaagactcc accaccagct cgctcgccgg
     7801 ctcgctgaat tccgcaatcg ccacagaatt cccgaacaca tcatgcagcc accgaaccga
     7861 ggccgccggc gagatcgtca acgcggtccc cagcaaccgc agatcatggc tgtcgcgcgg
     7921 ccgcatcatc atgcgatgcg ccccgaacgt caccggctgc gcgtacttgt attcagtccg
     7981 atgcgagacg ttgagaattt tcatcgatcc aactaacccg aacgcgcgac gttccccaca
     8041 tcaactatgc gttcgcgtcg gtcagcgcct ccagatgcac ttcaacagac agtgcctgct
     8101 cgccgccccc aagaatcacc ccacgcagtg gcgtcacatc gctgtaatcg cggccccaag
     8161 ccagcacgac gtgttcgtcc cgcaccacga tcccgttggt cggatcgaga tccacccacc
     8221 catgctccgg ccccagccat gcgccaatcc acgcatgcga ttgatcggca cccagccgcc
     8281 gaggctgccc ctctgccggc cgcgtccgga tatagccaga cacataccgt gccggcacgc
     8341 cgaacgcgcg gaccatgctg atcatcatgt gcgcgaagtc ctggcacacg ccctcgcgcc
     8401 gcctcagcac atcgcgtatg ggcgtcgccg tcgtcgtcac gcccggccgg aacgcgaaat
     8461 cccgcttgat ccgagcattc agttccagca gcgccgccag cgctggccgc cccggcagaa
     8521 agctcagcgc ggcataggcc cgcgcctccg gatcaatcgg cgccaaagga ctgtcgaacg
     8581 cgaactccgc cgcctcccac ccctcggccc cgcccatcgc cgcacgtcgc gccaccacct
     8641 cccacgccgg cgtcgccccc gccgccggca atccaggaaa tagcacatcc acggtcgcct
     8701 cagccgtcac ctcgaacgcc gcatgcgccg catccaggaa cagccaggtc acatcgttgc
     8761 cgaagtgatc gcgcccttca tgccgccagg aaactgcggg cgccgcccgc acctccgagt
     8821 gcaacacagc ctgcacgggc aaatgccgag gccgcagatg cgccagatgc accccaagat
     8881 ccgccgggct ccgatagtcg tatcgcgtgg tgtgacgcac ccgatagatc acgccgcgcc
     8941 ccgcatcgtc cgcgccggtg catccagccc gacactccgc aacgtcggca gcaccgcgaa
     9001 ataccgccgc gacaaccgat cggacagttc cgccaccgca tcacgcatcg agcgcagctc
     9061 atccggcagc agtacagccg ccttcgcctg gtccgccgcc tgggtaaccc gttgcaccat
     9121 cgccaccacg tcgccgagca acccgtcggt tgtcgccacc agcgactgat ctgtctgccc
     9181 cgcgatctcg gtcagcagca tccgcgtcgt cgccaactgg aacgccagcc cgcgcgggtt
     9241 cccctcatcc gccagcacca ggtccagcac cggcgccggc tgcaccaccg ccagataccg
     9301 gctgcgatac gtaatcgtgg aatcgcagag ctccagcgcc agtcgcagcc cgtgctccac
     9361 ccgcgccgac tgctccgccg cacccggaaa ctccagcgta cacgccacca cgtcggcaat
     9421 cga
//
LOCUS       DATGEM010000190        14759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_860404, whole genome shotgun sequence.
ACCESSION   DATGEM010000190 DATGEM010000000
VERSION     DATGEM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860404"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="gltD"
                     /locus_tag="VKQ27_06095"
     CDS             complement(<1..452)
                     /gene="gltD"
                     /locus_tag="VKQ27_06095"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HLY88538.1"
                     /translation="MGKVTGFLEIERHDRGYDKPDTRRKTWTEFLKPLPENELSEQAA
                     RCMDCGIPFCHQGCPVNNLIPDWNNLAYRHQWKTAIRVLHSTNNFPEFTGRVCPAPCE
                     AACTLNIEDNPVTIKTIECAIVDRAWSEGWIEPHVPAHRTGKRVAVVGS"
     gene            complement(456..5120)
                     /gene="gltB"
                     /locus_tag="VKQ27_06100"
     CDS             complement(456..5120)
                     /gene="gltB"
                     /locus_tag="VKQ27_06100"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HLY88539.1"
                     /translation="MMGSPAGPGLYDPRNEHDSCGVGFVANIKGRKSHDIITRGLQIL
                     VNLDHRGAVGADPLVGDGAGCLIQMPDKLLRAWADENGLKLPEPGRYAVAMCFLPQDA
                     ASREYAMKLFEHYITAEGQSLLGWRDVPTDLTGLGKRVIETMPVIRQAIVVASPKVKD
                     QNAFERKILTIRKQTLNNLRGIEVQRKLPGLSQLYMPSFSTRTVVYKGLLLAPQVGTF
                     YQDLRNPLTQSALALVHQRFSTNTFPSWRLAHPYRFLAHNGEINTVRGNVNWMHARRH
                     SMASELIGPDLDKMWPLIPTTGQSDTACIDNALEILVAGGYSLAHAMMMLIPEAWAEN
                     PLMDPERRAFYEYYAALMEPWDGPASIAFTDGRQIGATLDRNGLRPARYIVTDDDHVI
                     MASEAGVLPVPEERIVRKWRLQPGKMLLIDFEEGRIISDDEIKQNLARKHPYSEWLKA
                     TQVQLEELAEPSDSPGLMLELPPPRPNDAEALLNQQQAFGYTQEDIQFFLEPMAQVAD
                     DPLGSMGTDTPIAVLSSKPKLLYNYFKQNFAQVTNPPIDPIREALVMSLVSMIGPRPN
                     LLGHHAGSHYRLEVAQPILTNADLEKVRAISSLVGDAFRTKTIDTTWSAGEGVAGMAR
                     AVDRICGIATDAVLDGYNILILSDRAIGPARIPMPALLATAAVHHHLIRRGLRTSTGL
                     VVETGEAREVHHFCVLAGYGAEAINPYLAFETLEQIREQDGLQLKPYEVQKNYLKAIG
                     KGILKVMSKMGISTYQSYCGAQIFDAVGLSTDFIEKCFTGTASTIEGAGFDEVARECV
                     QRHQNAYGENPLYEGMLDAGGDYAFRLRGEDHAWTPDTVGKLQHAVRGNLQQEFTAYT
                     NVINDQSKRLLTLRGLMDLKFADTPVPLDEVEPASEIVKRFATGAMSFGSISREAHTT
                     LAIAMNRIGGRSNTGEGGEESDRYKPLPNGDSMRSAIKQVASGRFGVTIEYLVNADDL
                     QIKMAQGAKPGEGGQLPGRKVDKNIARVRFSTPGVGLISPPPHHDIYSIEDLAQLIYD
                     LKNANPKARISVKLVSEVGVGTVAAGVAKARSDHVTISGFEGGTGASPLTSLTHAGSP
                     WEIGLAETQQTLVLNGLRGRIAVQVDGGLRTGRDVVVGALLGADEFGFSTAPLIAAGC
                     IMMRKCHLNTCPVGIATQDPVLRKKFTGQPEHVINFFFFIAEEVRALMAKLGFRTFTE
                     MVGQVGKLDMRKAIDHWKAKGVDLSRILHQVPAREGVAIFNCEKQQHHLDRAMDNELI
                     ELAEPALQRAEPVRIERDIRNVHRTVGAMLSGEVAKRYGHTGLPEDTIQIGLRGNAGG
                     SFGAFLARGITLELTGDANDYVGKGLSGGRVVVKQPPVNREPTENIIIGNTVLYGAIA
                     GEAYFQGVAGERFAVRNSGAAAVVEGCGDHGCEYMTGGVVVVLGETGRNFAAGMSGGV
                     AYVYDPKRRFESLCNLTDVDLEPIRPTDAASDDPERPRQRSVSVEDNGMGDLLRFDAE
                     RLRVLVEKHLLYTGSARARELLDDWDNALACFVKVMPLDFRRALLDLRAERQAAKAAA
                     AE"
     gene            complement(5388..5855)
                     /locus_tag="VKQ27_06105"
     CDS             complement(5388..5855)
                     /locus_tag="VKQ27_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HLY88540.1"
                     /translation="MDETGDPDVVLRLTAQIVAAHVEHNPVEAAALPGLIEKVYQTLR
                     EVGLAPVESTKLSPAVPIKQSVKADFITCLEDGKKLKMLKRHLMTVYQMTPAQYRARW
                     GLPADYPMVAPNYAKVRSSLAKKIGLGRKSADTPAPAKRRGPKPATRGRRARA"
     gene            6034..6282
                     /locus_tag="VKQ27_06110"
     CDS             6034..6282
                     /locus_tag="VKQ27_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88541.1"
                     /translation="MSKYRPLQDYLRRQNGQRVELSFLEIERIIGGELPPSAYAPRWW
                     ISAPSSRRHALWQNAWHNEGYVATLLQGADCVEFRKQL"
     gene            complement(6306..8663)
                     /locus_tag="VKQ27_06115"
     CDS             complement(6306..8663)
                     /locus_tag="VKQ27_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HLY88542.1"
                     /translation="MTFEEQLRRDLLSLDQTLRILQYDWQRDPATFDLVTRADQVVVL
                     NDVSLQIFIADQQGIVRASTRAAIIGTNVSQRDYFHYEASLPASDGGMYIGALTQGQV
                     TRQWQLNLVRRLGHNDGTFAGVIAASYDPNALSRFHPDVASAGRVLIGLVSMKDGNAW
                     TFTDQAPGVVDIGHTKLFANMREANQESWRGQSGLEDADRLYAFATIPDRDLKVVVGV
                     DSAEAMRESAAWEHNALLFAAGTTIVMLLLALVLLRTLDAARRGHQALAHERAILEAA
                     LRGMSDGIMMVDRDLHLMAWNQHFPEFTGVPDGILRVGLPMEDILRSQVTAGEFGAVE
                     VEAEVSRRMSLIRSGASMGAMERPRPSGRQLEIRRNPLPGGGFVTLYSDVTARRQAEE
                     RMRQAQTMAALGRLTAGVAHDFNNLLVSISGNAELLQEQLAQWPEQAQRLATILQSAE
                     RGADLVQRLLAFGRRQALAPARVDLNDLVRGMRDLLAATLGRGIVVETKLADGLWPAL
                     ADPVQVEHSILNLAINARDAMPEGGKLTIETANMSWERAGATSPDLSPGDYLVISVAD
                     SGTGMDEEVLRNAFEPFFTTKPPGLGSGLGLSQVYGVANQSGGGVRIDSVVGVGTTVS
                     VFFPRAEKIAEPHSGLERAAAAMECNQPRRPGRTILVVDDEPECRATIADMLTTNGFV
                     VNVAASGAEALGLLDREMRFDLLLIDYMMPGMTGLELARIIRARAGENPPIVFLTGGD
                     GDWLSAERWVLIKPFLTRGLLDVVEVALDHARRENGARGPASQDA"
     gene            9037..10074
                     /locus_tag="VKQ27_06120"
     CDS             9037..10074
                     /locus_tag="VKQ27_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88543.1"
                     /translation="MPVTKFGRIFAPDEAWLAKALQEDILDRDLPIIDTHHHLWQRPD
                     HRYLLHDLIVDMRTGHNVVATVFLQCHAMYRASGPEEMRPVGETEFVAGIAAMSDSGG
                     YGSTRVAAGIVGYADLRLGERVEAVLEAQVRAGGGRFRGVRHSGNWDADPIIGNGAPG
                     PDIYKRADFRAGLARLSAMGLSLDAWTFHTQLGDVIDLARAFPATNIVMGHCGGVLGY
                     GPYDGKRDEIYAGWKANIVELAKCPNVTMKLGGMMMRAALFDYKGAERPVTSEELAAL
                     WRPYIEFCIEKFGPRRCMFESNFPVEKMGIGWVALWNAFKRIAAGASPDEKRALFHDT
                     AQRIYRLGSTT"
     gene            10071..10868
                     /locus_tag="VKQ27_06125"
     CDS             10071..10868
                     /locus_tag="VKQ27_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY88544.1"
                     /translation="MSDTAHRDQILYQFTRQATPFSTAGTITDANALRMIVAAAAPSP
                     DDTVLDVACGGGIVVCAFAPHVKRATGIDMTPAMLDRARQHAADTGVGNVAWDRGDVT
                     ALPYPDGAFNIVVTRFSMHHFLDPLGVLREMVRVCAPGGRVVMVDMYASDDTAKAAEW
                     NRAEKLRDPSHVRCLTLPELKALFPAVGLPQPGANFYELRDEIRNLLARSFPNPGDDV
                     KIIEMFAASAEDDRLGIPIHKIGDKINYAYRVAILSAPVIASPARAG"
     gene            complement(10949..11650)
                     /locus_tag="VKQ27_06130"
     CDS             complement(10949..11650)
                     /locus_tag="VKQ27_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88545.1"
                     /translation="MLEIDDIHVFYDKIEALKGISLVVDEGKIVTLVGGNGAGKSTTL
                     RAISGLLHPRHGSIRFDGRALAGMGAHEIASIGVVHVPEGRRIFGRLTVSENLDMGAF
                     TRHDRRAIGEDKEQMLVLFPRLRERLKQVAGTLSGGEQQMLAAARALMARPKVLLMDE
                     PSMGLAPMLVEQVFETIKRINAQGMTVLLVEQNAPMALGIADHGYVLESGRIVLDGPG
                     RDLLDDERVQRAYLG"
     gene            complement(11660..12442)
                     /locus_tag="VKQ27_06135"
     CDS             complement(11660..12442)
                     /locus_tag="VKQ27_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88546.1"
                     /translation="MTTLLQCNKVTMRFGGLVAISDVDLAVQENTIHSVIGPNGAGKT
                     TLFNCITQNLRPTSGEVWFGGARIDGLKPDRVAAAGMSRTYQNIRLFRNISALENLLV
                     GMHLHLRSTWLGAILHSPATMRDEREAHEEALRLLQFVGLRGRGDMLARNLAYGEQRR
                     LEIGRALATRPKLLLLDEPTAGMNPHETSDMMAFIQQVRERFGITILLIEHQMRVVMS
                     MSDRVTVLDHGVKIAEDVPARVQQDPAVIEAYLGKAARPAAE"
     gene            complement(12439..14091)
                     /locus_tag="VKQ27_06140"
     CDS             complement(12439..14091)
                     /locus_tag="VKQ27_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88547.1"
                     /translation="MIRRSVLIGLAFGAGAVHIAVVGVLLMLHQRWIVVDVLTLGQAA
                     LFLIAGGAGAMAGRPLSGLLAGAAAGVPIAALTVIMDAVPLQWMFIALSNDLFQMLTT
                     EQGLAVGCVILIGGGALAGLLGALWRISPAALRRAVGPGLIAVVVAGVFQELIQLILQ
                     QYEGGTVADLRDFIYTWEGLSPQGALTIFVVVAVCGEIFGSIWRRRAARLEVRDHRRE
                     RMVWAGLALAALVVLPALAGSYVGQVLMLVGLYILMGMGLNLEVGLAGLLDLGFVAFF
                     AVGAYSTALMTADSPHALMHLAYWEAMPIAVLLSIFVGVLFGLPVLGVRGDYLAVATM
                     GLGEIVRVIVQSDFAAPLLGGAQGVLQIPKPSIGNFLLGNPVSLFYLTLVASAVAAFV
                     AWRLENSRLGRAWMALRDDEDVAQALGINLIRSKLLAYGLGAAFAGLAGSIFATMLSS
                     VYPSSFQLLISINVLALIIVGGMGSLPGVVLGSIALIGLPELLREFGEYRYLFYGAVL
                     IVMMRLRPEGLWPSDVRQRELHATDETMALDAGTDALPAQPR"
     gene            complement(14088..>14759)
                     /locus_tag="VKQ27_06145"
     CDS             complement(14088..>14759)
                     /locus_tag="VKQ27_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88548.1"
                     /translation="ERIAYRPFRRVVGLAPLICAIGVSFFLEQTFRGMFGSAVKSYPS
                     PSWQRATFAVAGFNVPRVDVLVIGLALVFMGVLYLIVQRTKMGTAMRAVAEDGEAAAL
                     MGIDVDKVVVFTFILGGAMAGIAGVFYAFVYKQVYFFMGFTPGIKAFGAAVLGGIGNI
                     PGAMLGGFFLGMVESVGPTLFLDGLGIPAPYQLRDLIAFTLLIMVLIFRPQGLLGERM
                     ARKRA"
BASE COUNT         2726 a         4892 c         4430 g         2711 t
ORIGIN      
        1 gagcccacca cggcgacacg cttgccggtg cgatgcgccg gcacatgcgg ctcgatccag
       61 ccttctgacc acgctcgatc gacgatcgcg cactcgatgg ttttgatcgt gacaggattg
      121 tcctcgatgt tcagcgtgca ggcggcctcg cacggcgcgg ggcaaacgcg gccggtgaac
      181 tcggggaagt tgttggtgga atgcagcacc cggatcgcag tcttccactg gtgccgatag
      241 gcaagattgt tccagtccgg gatcagattg ttgaccggac agccctggtg gcagaacggt
      301 atgccgcaat ccatgcaccg cgccgcctgc tcggacagct cgttctcggg cagcggcttc
      361 aggaactcgg tccacgtctt gcgccgtgtg tccggcttat cgtagccgcg atcgtgccgc
      421 tcgatttcga ggaacccagt gactttgccc atggtttact ccgcggcggc ggcctttgcc
      481 gcctgacgtt cagcgcgcag atcgagcaaa gcgcgccgga agtcgagcgg catcaccttc
      541 acgaagcagg ccaacgcatt atcccaatca tccagcagtt cgcgtgcccg cgcactgccc
      601 gtgtacagca ggtgcttctc caccagcacg cgcagccgct ccgcatcaaa gcggagcaga
      661 tcgcccatgc cgttgtcctc gacggatacc gagcgctgcc ggggccgttc cggatcgtcg
      721 cttgccgcgt ccgtcgggcg gatcggttcc aagtccacat cggtgagatt gcacaggctc
      781 tcgaagcggc gcttcgggtc gtagacgtac gccacgccac cagacattcc ggccgcaaag
      841 ttgcgcccgg tctcgccgag taccacgacg acgccgccgg tcatgtattc acaaccgtgg
      901 tctccacagc cttccaccac cgccgccgcg cccgaattgc gcacggcgaa gcgctcaccc
      961 gcgacgccct ggaaatacgc ctcaccggcg attgcgccat agagcactgt gttgccaatg
     1021 atgatgttct cggtcggctc acggttcacc gggggctgct tcaccacgac gcgtccgccg
     1081 gacaggccct tgccgacata gtcgttcgcg tcgccggtca gctccagtgt gatcccgcgc
     1141 gcgaggaaag cgccgaaact accgccggcg ttgccacgca ggccaatctg gatggtgtcc
     1201 tccggcagac cggtgtggcc gtagcgcttc gccacctcgc ctgacagcat cgcgccgacg
     1261 gtgcggtgca cattgcggat gtcgcgctcg atgcggaccg gttcggcgcg ctgcaaggcg
     1321 ggctcggcca gttcgatcag ctcgttgtcc atcgcgcggt cgaggtgatg ctgctgcttc
     1381 tcgcagttga agatcgccac gccctctcgc gccggcacct gatgcaggat gcggctgaga
     1441 tcgacgccct tcgccttcca gtggtcgatc gccttcctca tgtcgagctt gccgacctga
     1501 ccgaccatct ccgtgaatgt acggaagccg agcttcgcca tcagcgcgcg cacttcttct
     1561 gcgatgaaga agaagaagtt gatcacgtgc tccggctggc cagtgaactt cttgcgcagt
     1621 accgggtcct gagtagcgat gccgaccggg caggtgttca ggtggcactt gcgcatcatg
     1681 atgcagcctg cggcgatcag cggcgcagtg gagaagccga actcgtcggc tcccagcagc
     1741 gcccccacga cgacatcgcg gcccgtgcgc agcccgccat ccacctgcac cgcgatacgg
     1801 ccgcgcagtc cattcagcac gagagtctgc tgcgtctccg ccagcccgat ctcccaaggc
     1861 gatccggcat gcgtgagcga cgtgagcggc gaagcgcccg tgccgccctc gaagccggag
     1921 attgttacat gatcggagcg ggctttcgcg acaccggctg cgaccgtgcc gacgcccacc
     1981 tccgatacga gcttgaccga gatacgcgct ttcggattgg cgtttttcag gtcgtagatt
     2041 agctgcgcca ggtcctcgat cgagtagatg tcgtggtgcg gaggcggtga gatcaggcca
     2101 acgcccggcg tggagaagcg gacccgtgcg atgttcttgt ccaccttgcg gccgggcaac
     2161 tgcccgcctt cgcccggctt cgcgccctgc gccatcttga tctgcagatc gtccgcattc
     2221 accaggtact cgatggtgac accgaagcgc ccggacgcga cctgcttgat cgccgatcgc
     2281 atcgaatcgc cgttcggcag aggcttgtag cgatcggatt cctcgccgcc ctcgccggtg
     2341 ttggaacgcc cgccgatgcg gttcatcgcg atcgccagcg tggtatgcgc ctcgcgcgag
     2401 atcgagccga agctcatcgc gcccgttgcg aaccgcttca cgatctccga ggcaggctcg
     2461 acttcatcaa gcggcacagg agtgtcggcg aacttcagat ccatcagccc gcgcaacgtc
     2521 agcagccgct tgctctgatc gttgatcaca ttggtatacg ctgtgaattc ctgctgcaaa
     2581 ttgccgcgca ccgcatgttg cagcttgccc acggtgtcgg gcgtccaggc gtggtcctcg
     2641 ccacgcaggc ggaatgcgta gtcgccgccg gcatcgagca ttccctcata cagcgggttt
     2701 tcgccatagg cgttctggtg acgctgaaca cattcgcgcg cgacttcgtc gaagcccgcg
     2761 ccctcgatcg tgcttgccgt accagtgaag cacttctcga tgaagtcggt ggacaggcca
     2821 accgcatcga agatctgtgc accgcaatag gactgatagg tcgagatgcc catcttggac
     2881 atcaccttca gaatgccctt gccgatcgct ttcaggtagt tcttctggac ctcgtaaggc
     2941 ttcagctgca gtccgtcctg ctcgcggatc tgctccagcg tttcgaacgc cagatatgga
     3001 ttgatcgcct ctgcaccata gccggccagc acacagaaat ggtgcacctc gcgcgcctcg
     3061 cccgtctcga ccacgaggcc ggtgctggtg cgcagaccgc ggcggatgag gtgatgatgc
     3121 accgccgccg tcgcgagcag cgccggcata ggaatgcgcg ccggaccaat cgcgcgatcc
     3181 gacaggatca ggatgttgta cccatcaagc accgcatcgg tcgcgatgcc gcatatacga
     3241 tcgactgcac gcgccatgcc ggcaacgcct tcgccagccg accacgtggt gtcaatcgtt
     3301 ttggtacgga acgcatcgcc caccagagac gatatggcgc gcaccttctc cagatcggcg
     3361 ttggtcagga tcggctgtgc cacttccagc cggtaatgcg agccggcgtg atgcccgagc
     3421 agattgggcc gcggaccgat catcgatacc agcgacatca ccaacgcctc gcggatcgga
     3481 tcgatcgggg ggttggtcac ctgggcaaag ttctgcttga agtagttgta cagcagcttc
     3541 ggcttgctcg acagcacggc gatcggcgta tcggtcccca tggagcccaa cggatcgtcc
     3601 gccacctgcg ccatcggctc caggaagaac tgtatatctt cctgcgtata cccaaatgct
     3661 tgctgttggt tcaggagcgc ctcggcgtcg ttcggccgcg gcggcggcag ctccagcatc
     3721 aggccaggcg agtcagacgg ctccgccagc tcctcgagct gtacttgtgt ggccttcagc
     3781 cactccgagt acggatgctt gcgcgcgagg ttctgcttga tctcgtcgtc gctgatgatg
     3841 cggccttcct cgaagtcgat cagcagcatc ttgcctggct gcaggcgcca tttacgcacg
     3901 atgcgctctt caggcaccgg cagcacgccc gcttctgatg ccatgatcac gtggtcatcg
     3961 tcggtcacga tgtaccgcgc cggacgcagc ccgttgcggt ccagcgtcgc gccgatctgc
     4021 cgcccatccg tgaaggcgat cgaggccggc ccgtcccacg gttccatcag cgccgcatag
     4081 tattcgtaga aggcacgccg ttccggatcc atcagcgggt tctccgccca ggcttccggg
     4141 atcagcatca tcatcgcgtg cgccagcgag taaccgccgg cgaccaggat ctccagcgcg
     4201 ttgtcgatac acgccgtatc cgactgaccc gtggtgggga tcagcggcca catcttgtcg
     4261 aggtccggcc cgatcagctc tgacgccatc gaatgacgcc gcgcatgcat ccaattcacg
     4321 ttgccgcgaa ctgtgttgat ctcgccgtta tgcgccagga agcggtacgg gtgtgccagc
     4381 cgccaggacg ggaacgtgtt ggtggagaag cgctgatgca ccaaggccag tgccgactgg
     4441 gtcaacggat tgcgcaggtc ctgatagaag gtaccgacct gcggcgccag tagcagcccc
     4501 ttgtagacca ccgtgcgcgt cgaaaacgac ggcatgtaaa gctggctcag cccgggcagt
     4561 tttcgctgca cctcgatgcc gcgcagattg ttcagcgtct gcttgcggat ggtaaggatc
     4621 ttccgctcga acgcgttctg gtccttcact ttaggactgg caacgacaat tgcctgccgg
     4681 atcactggca tcgtttcgat cacgcgcttg ccgagaccgg tcaggtctgt cggcacatcg
     4741 cgccaaccca gcaacgactg cccttcagcg gtaatatagt gttcaaacag cttcatcgcg
     4801 tactcgcgcg acgcggcatc ctgcggcagg aagcacatcg caaccgcgta gcgtcccggc
     4861 tccggcaatt tgaggccgtt ctcgtccgcc cacgcgcgca gcagcttgtc cggcatctgg
     4921 atcaggcagc cggcgccatc gcccaccagc gggtcggcgc cgacagcgcc ccggtggtcc
     4981 aggttcacca ggatctgcag gccacgcgtg atgatatcgt gcgactttcg gcccttgatg
     5041 ttggcaacga agcccacgcc gcacgagtcg tgctcgttgc gtggatcgta caaccctggt
     5101 cccgctgggg atcccatcat ggcctacact gcccccgtct acaccggcca cgcgacagcc
     5161 agccctattc aggctcgccc gcaaaccgac acgttagcgc ccgttttgct gcactgcaac
     5221 gaccaccgag gccgagtggc accagacctt ccgcggaggc cgccgccttg gccgaacctc
     5281 ctggccgtgc ggcgcttcaa tcacctctgg cagccgcgtg caacccccgg ctcccggcga
     5341 agtaaccgcg gcgcgccgtc agatccatca gtgagcggcg gacgtgccta agcccgcgcg
     5401 cgccgtcctc gggtggcagg cttcggtccc cgccgtttgg ccggcgcagg cgtgtccgct
     5461 gacttccgtc ccaagccgat cttcttcgcc agtgatgaac gcaccttcgc gtagttcggg
     5521 gcgaccatcg gatagtccgc tggcaagccc caccgcgctc gatattgtgc cggcgtcatc
     5581 tggtacaccg tcatcagatg tcgcttcagc atcttcagct tcttgccatc ctccaggcag
     5641 gtgatgaaat cggccttgac cgactgcttg ataggtacgg ccggtgacag cttcgttgac
     5701 tctacaggcg ccaggccgac ttctcgaagc gtctgatata ctttctcgat caatccaggc
     5761 agcgctgctg cctctactgg gttgtgttct acatgagcag caacgatctg ggcggtcagc
     5821 ctcaagacca catccggatc cccggtttca tccatctgaa caacctctat tcacgataga
     5881 ggctccatta caggaacaat acgccaattg ccaaattttt tgtcgcagat gagttttgac
     5941 acgggctgaa taaaccacca attcctgtat ccgaccagta agcttgtgtt aacctctgca
     6001 tgacatgcct tgcgccatgg aggaatctat ggcgtgagca aatatcgtcc attgcaggat
     6061 tacttgcggc ggcaaaacgg gcaaagagtc gagctcagtt ttctggagat cgagcgcatt
     6121 attggtggcg aactgcctcc gagcgcctat gcgccgcgtt ggtggatatc cgctccgtcg
     6181 agccggcgcc acgcgctttg gcagaacgca tggcacaacg agggttacgt cgctacattg
     6241 cttcagggag ctgattgcgt cgaatttcgt aagcaattgt gagagctcgt tactctcaag
     6301 tgaaatcacg catcctgcga cgccgggccg cgtgctccgt tctcgcgacg cgcgtgatca
     6361 agcgctacct ctacgacatc aagcaggccg cgtgtcagga atggctttat caacacccag
     6421 cgctctgccg acagccagtc gccatcacca cccgtcagga acacgatcgg cgggttttcg
     6481 ccggcgcgag cgcggatgat gcgagccagt tccagccccg tcatgccggg catcatgtag
     6541 tcgatcagca gcaggtcgaa gcgcatttcg cgatccagca agccaagcgc ctccgccccg
     6601 ctggctgcga cgtttacgac aaatccgttg gttgtgagca tatccgcaat cgtggcccgg
     6661 cactccggtt cgtcatccac gaccaggatg gtccgcccag gacggcgtgg ctggttgcat
     6721 tccatggcgg ccgccgcgcg ttccagcccc gagtgtggtt cggcaatctt ttccgctcgc
     6781 ggaaagaata cgctgacggt cgtgccgacg ccgacgacac tgtcgatccg caccccaccg
     6841 ccagactgat tcgccacgcc atagacctgg ctgaggccga gccctgagcc gaggccaggc
     6901 ggtttcgtgg taaaaaaggg ctcgaatgcg ttgcgcagca cctcttcgtc catgccagtg
     6961 ccgctgtccg ccacggagat cacgaggtaa tcgccaggcg agaggtcggg cgatgtcgcg
     7021 ccggcccgct cccacgacat gttagccgtc tcgatcgtca gcttgcctcc ctccggcatt
     7081 gcatcgcgcg cattgatcgc aaggttcagg atcgaatgct ccacctgcac cgggtcagcc
     7141 aatgcgggcc ataatccgtc cgccagcttt gtctccacga cgatgccgcg tcctagggtt
     7201 gcggcgagaa gatcgcgcat gccgcgcaca aggtcattga gatccacccg tgccggcgcc
     7261 aacgcctgcc tgcggccgaa tgccagaagg cgctgcacca gatcggcgcc gcgttccgcc
     7321 gactgcagga tcgtcgcgag ccgttgggcc tgctccggcc actgcgccaa ttgttcctgc
     7381 aacagctccg cgttgccgct gatcgagacc agcagattat tgaagtcgtg cgccacgccc
     7441 gcagtcagcc ttcccagggc agccatggtc tgcgcctggc gcatccgttc ttcggcctgc
     7501 cgacgcgcgg tgacatcgct gtaaagcgtg acaaagccgc cccccggcag tgggttgcgt
     7561 ctgatctcga gctggcgtcc gcttggccgc ggtcgctcca tcgcgcccat gcttgcaccg
     7621 gaacggatca ggctcatccg gcgcgatact tcggcctcga cttccaccgc gccgaattcg
     7681 ccggcggtga cctggctgcg caggatgtcc tccatgggca gcccgacacg caggatgccg
     7741 tccggcacac cagtgaactc tggaaaatgc tgattccacg ccatcaggtg gagatcgcga
     7801 tccaccatca tgatgccgtc gctcatgccc ctcagcgccg cttcgaggat cgcgcgttca
     7861 tgcgccaggg cctgatgtcc ccgccgtgcc gcgtccaagg tccgtaacag cacaagggcc
     7921 agcaacagca tcacgatcgt ggtgccggca gcaaacagca gcgcattatg ctcccaggcg
     7981 gccgactcgc gcatcgcttc cgcgctgtcg acgccgacca caactttcag atcgcggtcc
     8041 ggtatcgttg cgaacgcgta caatcgatcg gcatcctcca atcccgattg cccgcgccag
     8101 ctttcctgat tggcttcccg catgttcgcg aacagctttg tgtgcccgat gtcgacaacg
     8161 cctggtgcct gatcggtgaa cgtccaggcg ttgccatcct tcatgctgac caaaccgatc
     8221 agtacccgcc cagcgctcgc tacgtcggga tgaaaccgcg acagtgcgtt ggggtcgtat
     8281 gacgccgcga tgaccccggc gaatgtccca tcattatggc caagccgccg caccagattg
     8341 agctgccatt gccgggtcac ctggccctgt gtcagcgcgc cgatatacat cccgccatca
     8401 ctggccggca gcgaggcctc ataatgaaaa taatcacgct ggctgacgtt cgtgccgatg
     8461 atcgccgcgc gtgtactggc gcgcacgata ccctgctggt cagcgataaa gatctgcaac
     8521 gagacgtcgt tcagcacgac cacctggtct gcgcgtgtta ccagatcgaa cgtggcgggg
     8581 tcgcgttgcc agtcatactg caggatgcgc aaagtctggt cgagtgacag caggtcgcgg
     8641 cgcaactgtt cctcgaaggt cattgccttg gcgagcaact ccgcccgcac cctcgcttca
     8701 gcttcggcct gatgcgcctg gatggcatca cgtgtgccaa gccatgtcag cgtgatcagt
     8761 acgagcgcgg cgatgatgac ggcagcctgc cctagccggc ggatcccggc tccctggttc
     8821 ggcggcttgg cacccccatc gcctgggggg cattgcgtcg cgctgatgac atcctccttt
     8881 caggcagtgc gatgcgcccc gtctgcatca cgtagtcgcg agcggtgatt tagcccattt
     8941 cgcccgcgtc gcgttggaaa aatgcgcgcc ttgcggttcg ctccgtggcc tggaacgtgc
     9001 taggccgcca tgtgagacac gtcggggaga accgtcatgc cggtgaccaa attcggccga
     9061 atattcgcgc ctgatgaggc atggctggca aaagcgcttc aggaagatat tttagatcgc
     9121 gatctgccga tcatcgatac gcatcaccat ctctggcagc gtccagatca tcgatacttg
     9181 ttgcacgatt tgatcgtcga catgcggacc ggccacaacg ttgttgcgac cgtgtttctg
     9241 caatgccatg ccatgtatcg cgcgagcgga cctgaagaga tgcgaccagt cggcgaaacc
     9301 gagttcgtcg ccggcatcgc ggcgatgagc gacagcggcg gttacggcag cacacgcgtg
     9361 gccgccggga tcgttggcta tgccgatttg aggctcggtg agcgggtcga ggcggtcctg
     9421 gaggcgcagg tcagggccgg cggtgggcgg ttccgcggcg tgcgccactc cggcaactgg
     9481 gatgctgacc cgatcatcgg caacggggcg ccagggccag atatctacaa gcgtgctgac
     9541 ttccgtgcgg ggctggcgcg gctgagcgca atggggctgt cattggatgc ctggacgttt
     9601 cacacgcaac ttggcgacgt catcgatctg gcgcgtgcgt tcccggcgac aaacatcgtc
     9661 atggggcatt gtggcggcgt gctgggctat gggccctatg acgggaagcg cgacgagatc
     9721 tatgccggct ggaaagccaa tatcgtcgaa ttggcgaaat gcccgaacgt gacgatgaag
     9781 ctcggcggca tgatgatgcg cgcagcgctg ttcgactaca aaggcgctga gcggccggtc
     9841 acgtcggagg aactggccgc attgtggcgg ccgtatatcg agttctgcat cgagaagttc
     9901 ggtccccggc gctgcatgtt cgagagcaac ttcccggtgg aaaagatggg cataggctgg
     9961 gtcgcgctgt ggaacgcgtt caagcgcatc gccgctggcg catcgcccga tgagaagcgc
    10021 gcgctgttcc atgatacggc acagcgtatc taccgattgg gatcaacgac atgagcgaca
    10081 cagcccatcg ggaccagatc ctgtaccagt tcacgcggca ggcgacaccg ttcagcacgg
    10141 ccggcacgat cacggatgcg aatgcgctgc gcatgatcgt tgcggcagcg gcgccgtctc
    10201 ccgacgatac agtgctggat gtcgcgtgtg gtggcggcat cgttgtttgc gccttcgcgc
    10261 cacatgtgaa gcgtgcaact ggcatcgata tgacgcctgc aatgctggat cgcgcgcgcc
    10321 agcacgcggc cgacacgggt gttggcaatg tcgcatggga ccgcggcgac gtgaccgcgc
    10381 tgccctatcc ggatggggcg ttcaacatcg tggtgacgcg gttttccatg caccattttc
    10441 tcgatccgct gggtgtgctg cgagagatgg tgcgcgtgtg cgcgccgggc ggacgtgtcg
    10501 ttatggtgga catgtatgcc tcggacgata cggcgaaggc tgcggaatgg aaccgggcag
    10561 agaagctgcg cgatccgtcg cacgtgcggt gcctgacgct gccggaactg aaggcgctgt
    10621 ttcctgcagt ggggctgccg cagcctggcg cgaacttcta tgaactgcgt gacgagatca
    10681 ggaatctgct ggcgcgttcg tttccgaacc cgggcgacga cgtgaagatc atcgaaatgt
    10741 tcgctgcgtc ggccgaggat gatcggctgg ggattccaat tcacaagatc ggtgacaaga
    10801 tcaactatgc ctatcgggtg gcgattcttt ccgcccctgt cattgcgagc ccggcgagag
    10861 ccgggtgaag caatctcggc aggtcaggtg cgcgaggccc cccgagattg cttcacccgg
    10921 ctctcgccgg gcgcgcaatg acaggcggct atcccaaata ggcccgctgc acccgttcat
    10981 catcgagcag gtctcggccg gggccatcaa gcacgatccg gccgctttcc agcacataac
    11041 catgatctgc gatgcccagc gccatcggcg cattctgttc caccagaagg accgtcattc
    11101 cctgcgcatt gatccgcttg atcgtctcga acacctgctc aacgagcatc ggggccaggc
    11161 ccatgctggg ttcgtccatc agcagcactt tgggcctcgc catcagcgcg cgcgccgccg
    11221 cgagcatctg ctgctcgcca cccgacaacg tgccggccac ctgcttcaga cgctcgcgca
    11281 gacgcgggaa cagcaccagc atctgttcct tgtcctcgcc gatcgcgcgg cggtcgtggc
    11341 gggtgaaggc gcccatatcc aggttctcgc tcaccgtcag gcggccgaag atccgccgcc
    11401 cctccggcac gtgcaccacg cctatcgagg cgatctcgtg cgcgcccatt cctgccagtg
    11461 cgcggccatc gaaccggatc gacccgtgac gcgggtggag caggccagaa atcgcgcgta
    11521 gagtcgtcga cttgccggcg ccattcccgc cgaccaacgt gacaatcttt ccttcatcca
    11581 cgacgagaga aatgcccttc agggcctcga tcttgtcgta gaacacatgg atatcgtcga
    11641 tctccagcag ggccatcttt cactccgccg ccggccgcgc ggccttgcct agataggcct
    11701 cgatcaccgc tgggtcctgc tgcacgcgcg ccggcacgtc ctcggcgatc ttcacgccgt
    11761 ggtccagcac cgtcacgcga tccgacatcg acatcactac gcgcatctgg tgctcgatca
    11821 gcaggatggt gatgccgaag cgttctcgca cctgctggat gaacgccatc atgtctgagg
    11881 tctcgtgcgg gttcatcccc gctgtcggct catccagcag cagcagcttc ggcctggtgg
    11941 ccagcgcgcg cccaatctcc aggcgtcgct gctcgccata ggcaagattg cgcgccagca
    12001 tatcgccgcg cccccgcagg ccgacgaatt gcagcaggcg cagcgcctcc tcgtgcgcct
    12061 cgcgttcgtc gcgcatagtg gccggtgagt gcaggatcgc ccccagccag gtggaacgta
    12121 gatgcaaatg catacccacc agcaggttct ccagcgcgga gatgtttcgg aagagcctga
    12181 tgttctggta agtgcggctc attcccgccg ccgcgacgcg atccggcttc aggccgtcga
    12241 tacgcgcgcc gccgaaccac acttcgccag aggtcgggcg caggttctgc gtgatgcaat
    12301 tgaacagcgt ggtcttgcct gcgccattcg ggccgatcac gctgtggatg gtgttctcct
    12361 gcaccgcgag atcgacgtcg ctgatggcca ccagcccgcc gaagcgcatg gtgaccttgt
    12421 tgcactggag cagtgtggtc atcgcggttg cgccggcagt gcgtctgtcc cggcgtcgag
    12481 cgccatcgtt tcatccgttg cgtgcagctc ccgctgacgc acatcagatg gccacaaacc
    12541 ctccggtcgc aagcgcatca tgacgatcag aactgcgcca tagaacagat agcggtactc
    12601 gccgaattcg cgcagcaatt cgggcaagcc gatcagcgcg atcgatccca gcaccacgcc
    12661 gggcaggctg cccatgccgc cgacaatgat caacgccagc acattgatgg agatcagcag
    12721 ctggaagctc gacgggtaca ccgagctcag catcgtcgcg aagatcgatc ccgccaggcc
    12781 ggcgaatgcc gcgcccagcc cataggccag cagcttggag cggatcaggt tgatgcccag
    12841 tgcctgcgcc acgtcctcat catcacgcaa cgccatccag gcgcggccca gacgcgaatt
    12901 ctccaaccgc cacgcgacga aagcggcaac tgcggatgcc accagcgtca ggtagaacag
    12961 cgacacggga ttgccgagca ggaaattccc aatgctgggc ttggggatct gcagaacacc
    13021 ttgcgcgcca cccagtagcg gcgcggcgaa gtccgactgc acgatcacgc gcacgatctc
    13081 gcccaggccc attgtcgcga cggccaggta gtcgcctcgc acgccaagca ccggcaggcc
    13141 gaacagcacg cccacgaaga tggacagcag tacagcgatc ggcatcgcct cccagtaggc
    13201 gaggtgcatc agcgcgtgtg gcgaatccgc cgtcatcaat gcggtcgaat aggcgcccac
    13261 agcgaagaat gcgacgaagc caaggtccag caggccggct aggccgacct ccaggttcag
    13321 gcccatgccc ataaggatgt atagaccaac cagcatcagg acctgaccga catacgaccc
    13381 ggccaacgcg ggcagcacta ccaaggctgc gagcgccagt cccgcccaca ccatgcgttc
    13441 acgccggtgg tcgcgcactt ccaggcgtgc tgcgcgccgg cgccagattg agccaaagat
    13501 ctcgccgcag accgccacaa ccacgaagat cgtcagtgcc ccctgcgggc tgaggccctc
    13561 ccaggtatag atgaagtcgc ggagatcagc gaccgtccct ccttcatatt gctgaaggat
    13621 gagctggatc agttcctgaa acacgccggc gacaaccact gcgatcagcc ccggcccaac
    13681 cgcacgccgc aacgccgcgg ggctaattcg ccataatgcg cccagcagtc cggccagcgc
    13741 tccgccaccg atcagaatga cgcagccgac tgccaggccc tgctctgttg tcagcatctg
    13801 gaacaggtcg ttcgacagcg cgatgaacat ccattgcagc ggcacggcat ccatgatcac
    13861 cgtcagtgcc gctatcggca cccccgcagc ggcaccagcc aacaggccgc tcaatggccg
    13921 gccggccatc gcgccggcac cgcccgcgat gaggaacagc gccgcctggc ccagggttag
    13981 aacgtcgacc acgatccatc gctggtgcag catcagcaac acgcccacca ccgcgatgtg
    14041 caccgcacca gcaccgaacg cgagcccgat caacacagag cgccggatca tgcgcgcttc
    14101 ctcgccatgc gttcgcccag cagtccctgc ggccggaaga tcaacaccat gatcagcaac
    14161 gtgaatgcga tcagatcgcg caactggtag ggcgcgggga tgcccagacc gtcgaggaac
    14221 agcgtcggcc ccaccgactc gaccattcct aggaagaaac cacccagcat tgcgcctgga
    14281 atgttgccga tacctccgag caccgcggcg ccgaatgcct tgataccggg cgtgaagccc
    14341 atgaagaagt atacctgctt gtagacgaag gcgtagaaca cgcccgcgat gccggccatc
    14401 gcgccaccga gtatgaaggt gaacaccacc accttgtcga cgtcgatgcc catcagtgcc
    14461 gcggcctcgc cgtcctccgc gaccgcgcgc atcgccgtgc ccatcttcgt gcgctggacg
    14521 atcaggtaaa gcacgcccat gaacacgagc gccaggccga tcaccagcac atcgacgcgc
    14581 ggcacgttga atcccgccac cgcaaacgtg gcgcgctgcc aggacggtga tggataggac
    14641 ttcacggctg acccgaacat tccgcggaat gtctgttcca ggaagaacga cacgccgatg
    14701 gcgcagatca atggcgccag ccccaccacg cggcggaacg gacgataggc aatgcgctc
//
LOCUS       DATGEM010000191         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_63050, whole genome shotgun sequence.
ACCESSION   DATGEM010000191 DATGEM010000000
VERSION     DATGEM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63050"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VKQ27_06150"
     CDS             <1..569
                     /locus_tag="VKQ27_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HLY88549.1"
                     /translation="LSLLPDSFAMEVVMRMLRMESVQKDVLDGVERTLRMEFMSNLAR
                     STRRDAHEMMADIFNNLDRQSETRFLAALEERNREAAERIKALMFTFDDLLRLTPQAV
                     QVLLRSVEKDKLPVAMKGASERLRDMFFKNMSERAGKILKDDIEALGPVRLRDVDEAQ
                     ASIVVVAKELAAQGAIEIAESQDEEMVY"
     gene            572..1219
                     /locus_tag="VKQ27_06155"
     CDS             572..1219
                     /locus_tag="VKQ27_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88550.1"
                     /translation="MDGFARVSDGNLLFAEDFDLADAVPEPQVIEPEVIEPTYTFGEV
                     SEAREAAWRDGHSAGLREAAADSVAAVHETATVIATQLAEERELAAARAEESAASIAR
                     LLIDSLDATFPALCARYGEAEVRSLVRVIVPALTQEPCITVRSHPRTAEAVAEEIARL
                     DPELSTRVQTVACDAMAPGDVRVIWRNGVATRDATALWEQVVAVLAPIKEMTDGD"
     gene            1209..1535
                     /gene="fliN"
                     /locus_tag="VKQ27_06160"
     CDS             1209..1535
                     /gene="fliN"
                     /locus_tag="VKQ27_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HLY88551.1"
                     /translation="MATEMELAELAEGAGSNNDPNSPRGARELEAVYDIPVTVSAVLG
                     KATMQVSQLLKLGRGAVVELDRKLGEAIDIYVNNRLVARGEVVMVDDSRLGVTMTEIV
                     KADRAS"
     gene            1535..2863
                     /locus_tag="VKQ27_06165"
     CDS             1535..2863
                     /locus_tag="VKQ27_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLY88552.1"
                     /translation="MRVLIIGSLAGEIGQAGRIAIARGAKLDQADDTETALARLRADA
                     RVGLVLCDIVHDVGAVVRALAAERMAVPVVACGTGDDAEAAAQAIRDGAREFLPLPPD
                     PDLIAAILEAAAGETHAMIVRDPVMQAAVHRAEQVAKSEASVLITGESGTGKEVLARH
                     IHRRSRRSGGPFVALNCAAIPDNLLESELFGHEKGAFSGAVARRIGKFEAANGGTLLL
                     DEISEMDVRLQAKLLRALQEKEIDRLGGASPVQVNARILATTNRDLAVEAQRGTFRED
                     LYFRLNVVSLRIPPLRERPGDIAALADHFARRYAEVNGVPYRPLSQTAMLRLTSHVWR
                     GNVRELENTIHRAVLLAEGPEIGIEAIEIGPPAASPGPTGISGIASQVGRRMDEVERD
                     LIIETLGHTLGNRTHAATILGISIRALRNKLRDYAAQGVVVPPPVAGVAA"
     gene            2885..>3717
                     /locus_tag="VKQ27_06170"
     CDS             2885..>3717
                     /locus_tag="VKQ27_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLY88553.1"
                     /translation="MSDTITATLLPAALAARLRQYTPGSDVGLAFGVVALLSVLILPL
                     PTILLDFGLAVSITAAVLVLMVALFLDRPLDFSSFPTLLLLSTLLRLSLNVAVTRLIL
                     SHGNEGPLAAGHVVAAFGGFLMGGDVVIGLIIFSILLVVNFMVITKGSGRIAEVAARF
                     SLDSMPGKQMAIDADMSAGIIDEKEARRRRRELEAESSFYGAMDGAAKFVRGDAIAAL
                     IITVINILGGLAIGLLRHGMTFSDAAATFTTLTAGDGLVSQIPALLVSTAAGIVVTKG
                     G"
BASE COUNT          623 a         1078 c         1345 g          671 t
ORIGIN      
        1 tgctgagttt gctgccagac tcgttcgcca tggaagttgt gatgcgcatg ctgcgcatgg
       61 agagtgtgca gaaggacgtg ctcgacgggg tcgagcgcac attgcgcatg gaattcatgt
      121 ccaatctggc gcgcagcacg cggcgtgacg cgcacgagat gatggcggac atcttcaaca
      181 acctggaccg tcaatccgag acgcgtttcc tcgccgccct ggaggagcgc aatcgcgagg
      241 ccgcggaacg catcaaggcg ctgatgttca cgttcgatga cctgctgcgg cttacgccgc
      301 aagccgtgca ggtgctgctg cgctcggtgg agaaggacaa gcttccagtt gcgatgaagg
      361 gcgcatcgga gaggctacgc gacatgttct tcaagaacat gtcggaacgt gccggcaaga
      421 tcctgaagga cgatatcgag gcacttggcc cggtaaggct gcgcgacgtg gacgaagcgc
      481 aggccagcat cgtggtggtg gcaaaggagc ttgcggcgca aggcgcgatc gagatcgccg
      541 aaagccagga tgaggagatg gtgtactgac catggacggg ttcgcacgtg tttccgacgg
      601 gaatctgctg ttcgccgagg attttgatct ggccgacgca gtgcccgagc cgcaggtgat
      661 cgagcctgag gtgatcgagc cgacatatac cttcggggag gttagtgaag cgcgcgaggc
      721 cgcgtggcgc gacgggcatt cggccggttt gcgggaagcg gccgcagaca gcgtggcggc
      781 ggtacacgaa acggcgacgg tgatcgccac gcagcttgct gaggaacgcg agttggccgc
      841 ggcgcgcgcc gaggagtccg ccgcatcgat cgcacggctt ctgatcgata gcctggatgc
      901 gacgttccca gcgttgtgcg cacgctatgg cgaggccgag gtgcgctcgc tcgtgcgcgt
      961 aatcgtgccg gctctgacac aggagccgtg catcaccgtg cgttcgcatc cacgcacggc
     1021 ggaggctgtc gcggaagaga tcgcgcggct cgacccggag ctttcgacgc gtgtgcaaac
     1081 ggtggcctgt gacgcgatgg cgccaggcga cgtccgggtg atctggcgga acggggtggc
     1141 gacgcgcgac gccacggcgc tgtgggaaca ggtcgtggcc gtgctggcac cgatcaagga
     1201 gatgaccgat ggcgactgaa atggaactgg ccgaactcgc tgagggagca ggctccaata
     1261 atgatccgaa ttcgccccgt ggcgcacgtg aactggaggc ggtgtacgac attccggtca
     1321 ccgtcagtgc ggtgcttggc aaagcgacca tgcaggtgag ccagctgttg aagctggggc
     1381 gcggtgcggt ggtggagctg gatcgcaagt tgggcgaggc gatcgacatc tatgtcaaca
     1441 atcgcctggt cgcgcgtggt gaggtggtga tggtggatga cagccgcctg ggcgtgacga
     1501 tgacggaaat cgtcaaggcg gacagggcga gctgatgcgc gtcctgatca tcgggtcgct
     1561 tgctggcgag atcggccagg cggggcggat cgccatagcg cgtggcgcga aactggacca
     1621 ggcggatgac accgaaactg ccttggcgcg cttgcgtgcc gatgcgcggg tcggcctggt
     1681 cctgtgcgac atcgtgcacg atgtgggcgc ggtggtgcgg gcactcgccg ccgagcgcat
     1741 ggccgtgccg gtggtggcat gcggcacggg cgacgatgcc gaggcggccg cgcaggcgat
     1801 ccgcgacggc gcgcgcgagt ttctgccgtt gccgccagat cccgacctga tcgctgctat
     1861 actggaagcg gcggctggcg agacgcacgc gatgatcgtg cgcgatccgg tcatgcaggc
     1921 ggccgtgcac cgtgcggagc aggtggcgaa gtcggaagcc tcggtgctga tcaccggcga
     1981 atcgggcacc ggcaaggagg tgctggcgcg gcacatccat cggcgttcac gtcgatccgg
     2041 cggaccattc gttgcgctga actgtgcggc gatccccgac aatctgctgg agtcggagct
     2101 gttcggccac gagaaggggg ccttctcggg tgccgtggcg cgacgcatag gcaaattcga
     2161 agcagcgaac ggcggaacgc tgctgctgga cgagatcagc gagatggatg tgcgcctgca
     2221 ggccaagctg ttgcgggcct tgcaggagaa ggagatagac cggctgggtg gcgcgtcgcc
     2281 ggtgcaggtg aatgcgcgca tcctggcgac gacgaaccgt gatctggcgg tcgaggcaca
     2341 acgcgggacc ttccgcgagg atctgtattt ccggctgaat gtcgtgtcgc tgcggatccc
     2401 cccgctgcgc gaacggccag gcgacatcgc ggcgctcgcg gatcacttcg cgcggcgtta
     2461 tgccgaggtg aacggcgttc cgtaccgtcc gctgtcgcag acggcgatgt tacgcctcac
     2521 gtcgcacgtc tggcgcggca acgtgcgtga actggagaat acgatccatc gcgctgtgct
     2581 gctggcggaa ggacccgaaa tcggcatcga ggcgatcgag atcgggcctc ctgccgcctc
     2641 cccggggccc actgggatct ccggcattgc ctcccaggtc ggacgccgga tggatgaagt
     2701 ggagcgtgat ctgatcatcg agacgcttgg ccatacgctg gggaatcgga ctcacgcggc
     2761 gaccatcctg ggcatctcga tccgtgcgct gcgcaacaag ctgcgggact atgcggcgca
     2821 gggcgtggtg gtgcccccgc cagtggctgg cgttgcggcg taacgatgag cgggcggcgt
     2881 cgccatgtct gacacaatca ccgcgacgct cctgcctgcc gccctggccg cacgcctgcg
     2941 ccagtatacg ccgggcagtg atgtcggcct ggcgtttggc gtggttgcgc tgctgtcggt
     3001 gctgatcctg ccgctgccca ccattctgct ggattttggg cttgcggtct cgatcaccgc
     3061 ggctgttctc gtgctgatgg tggcgctctt cctcgatcgc ccgttggatt tttcctcttt
     3121 cccgacgctc ctgctgttga gcacgctgct gcggctgtcg ctgaacgttg ccgtgacgag
     3181 gctgatcctg tcgcacggca atgaaggccc tctcgcggcc ggccatgtcg ttgcggcgtt
     3241 cggcggcttc ctgatgggcg gtgacgtggt gatcgggctg atcatcttct cgatcctgct
     3301 ggtggtgaac ttcatggtca tcaccaaagg ttctggccgc attgccgagg tcgctgcccg
     3361 gttcagcctg gattcgatgc cgggcaagca gatggcgatc gatgccgaca tgtctgccgg
     3421 catcatcgac gagaaggagg cgcgccggcg gcgccgcgaa ctcgaggccg aatccagctt
     3481 ctacggcgcg atggatgggg cggcgaaatt cgtgcgtggc gacgcgatcg cggcgctgat
     3541 catcacggtg atcaacattc taggcggact ggctatcgga ctgctccgcc atggcatgac
     3601 cttcagcgac gcagcggcga cgttcaccac gctaacggcg ggcgatgggt tggtgtcgca
     3661 gatccccgcg ctgctggtgt ccaccgccgc cggcattgtg gtgaccaaag gcggcat
//
LOCUS       DATGEM010000192         5402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_976129, whole genome shotgun sequence.
ACCESSION   DATGEM010000192 DATGEM010000000
VERSION     DATGEM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976129"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VKQ27_06175"
     CDS             <1..293
                     /locus_tag="VKQ27_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88554.1"
                     /translation="DLPAPVLDRRSKGGPDGFLLDIFHEHRQQLREQLLDGLLCHHHV
                     IDGWAVEAALAREGPVRNTDYLRLLQLADAEAWCRMRLACRAAAWSEAAGRI"
     gene            complement(315..2456)
                     /locus_tag="VKQ27_06180"
     CDS             complement(315..2456)
                     /locus_tag="VKQ27_06180"
                     /inference="COORDINATES: protein motif:HMM:NF033523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Atxe2 family lasso peptide isopeptidase"
                     /protein_id="HLY88555.1"
                     /translation="MIGLVLAVAAAIHPTDADPIDRCQRLLPQSVVSVPASRQSITIE
                     DLMQVRDIGQPGVEAAGLSLSPDHRKVAFQLRQADPGSNSYCQAIIVVSLSTRAPSVI
                     DRGGEFMALTYPRYGIAEYPSGIPALVTPRWSPDGRWLAYLRRDHGLTAAWIAHSDGG
                     DARAVSPGNLDLADLRWSADGRRLILSARPMLVEAEQAIDREGLSGYHIDDRVVPMAG
                     YRPFPTGPLPRVALALDLATNSLGTASASDAALLAGDRSPGNPPSAHLFASARDGRQA
                     WTAPDSSAYDAPEPLHVRTTKGAVRACGAPACDGHIIGLWWIGGHLFFLHREGPGYGD
                     LGFYRWDVGNGEPRRVFQTDHLLIDCTSVDAQLLCADEASLQPRRIVLVDIHSGRMRT
                     VFDPNPQAFQFALGPVRRLIVAPDEGPPTFADLVLPPHADVRHRYPLVVVQYVSRGLL
                     RGGTGDNYPILPLAAHGFAVLSFQRPPQLPVDAHATSMAEAFRQDTRDWRDRRNVQSA
                     LSRAIDAAIQTGTVDPARLGISGQSDGTATIAFALIGTRIFKAAAMSTCCEDSFSVMT
                     APGLNGTPLYRAAGFPSLTSPNPDFWKPASIAANAKSLDTPLLLQLSDDEATLGLQSF
                     MSLKEQRKPVDMYVFPDERHATWQPAHRLAIYRRSIAWFEFWLQGIEDHACASLDEMD
                     RWRDMRSLWQQAGRPTSSSGATNVSLPRAEP"
     gene            complement(2453..5062)
                     /locus_tag="VKQ27_06185"
     CDS             complement(2453..5062)
                     /locus_tag="VKQ27_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY88556.1"
                     /translation="MENIKSRLHRGLLYGAACAALALPSVGWAQAASRAHYHQPSLPM
                     AQALRALAANSNAQVLFSPGDVAGLTAPRLEGDFTVHEAVERLIQGTSLSADTSDGAI
                     IIRGRGTPPAEEVTGTAEQPSAIVVTGSRIRGAPVASQVTTLTREDMRNAGQNNLGDV
                     IRDIPQNAPGGQNPGVVMGSSRVTDTNVNSTSSLNLRGLGPDATLTLINGHRASYDSA
                     IQSVDVSSIPLLAVDRIEVVADGASALYGSDAVGGVANVILRQDYDGLYTSARIGSTT
                     RGGDFEQQYDALTGKRWSGGGFMASAEYGRSSAVTAGDRSFTSAMDPSATLYPRQRYV
                     DLVAAGHQALAPGVRFDVDGGYNERRTLIVVPLTTSGGFQANGTVATPRVRAFSVSPR
                     LTFDLPAGWRAVLSGTYGKDSTRLDTRYFFAGSVLESFPGHYDNSLKVVELSAEGPLF
                     RLPGGDARVVLGGGYRSNHLDLYLATAFGNIVEPNTVFDRSRNAAYGYGEIYLPFVSP
                     ALDIGGIHSLSASAAVRYESYLDVDHVATPKLGLIYAPVDGVELKASWGKSFKAPTLY
                     QQFSGTRAVLVDPIFFGASGYPAGSTILLREGSNPDLKPEKATSWTTTLSLHPTFVPG
                     FKLDVSYFHVAYRNRIVQPLVNDDGALSDPIYRSVITTNPSAELQQAAISDAAVGLEN
                     FAGVAYNPNAVAVLFDDRFRNAARQKINGVDLSVDYKLDLSAGRSLALTGLATYLDSR
                     QQLSGEQPLTALSGTIFNPPRWRGRFGAIWSSKSLTASTYVNVIGRLTDNRSAPVRAI
                     GGMTTADATIRYHAPDNRGLPGGLDVTFSVTNFTDAKPPHIIVSAPYAPPYDSTNHSA
                     LGRAVSLTVGKRW"
     gene            complement(5143..>5402)
                     /locus_tag="VKQ27_06190"
     CDS             complement(5143..>5402)
                     /locus_tag="VKQ27_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88557.1"
                     /translation="RLLQDTAWPTGMIAFDDASLADVIARANGYATTPIVLGDPSLGS
                     LRVSGRFRIDEPAGLSRNLARLFGLSIDTSRPDRIILDSSG"
BASE COUNT          995 a         1570 c         1924 g          913 t
ORIGIN      
        1 atgatctgcc cgcgccggtc ctcgatcgcc gttccaaggg agggcccgac ggcttcctgc
       61 tcgatatctt ccacgagcat cggcagcaat tgcgcgaaca actgctcgac ggcctgctgt
      121 gtcaccacca tgtcatcgac gggtgggcgg tcgaggcggc gctggcgaga gaaggcccag
      181 tacgcaatac cgactatctc cgcctgctgc agttggccga tgcggaggcc tggtgccgga
      241 tgcgcttggc ttgccgtgcc gcagcgtgga gcgaagctgc cggaaggatt tgagggatgc
      301 gaaagccgtg accatcatgg ttcggcgcgc gggagagaga cgttggtagc accactcgat
      361 gaggtgggac ggccggcttg ttgccaaagc gaccgcatgt cccgccagcg atccatctca
      421 tcaagcgagg cgcaagcatg gtcttcgatg ccttgtagcc agaattcgaa ccacgcgatg
      481 ctgcgccggt agatggcgag ccgatgggcg ggctgccatg tggcgtgacg ctcgtcaggg
      541 aacacataca tatcgacggg cttgcgctgt tccttgagcg acatgaagga ctggaggcca
      601 agcgtcgcct catcgtcgga gagctgcagc agcaagggcg tatcgagcga ctttgcattg
      661 gcggcgatcg acgcgggttt ccagaagtcg ggattggggg aggtcagcga cgggaaaccg
      721 gcggcgcgat agagtggagt gccgttcagg cctggggcgg tcatcacgct gaaactgtct
      781 tcgcaacagg tgctcattgc cgccgccttg aatatcctgg tgccgatcag cgcgaaggcg
      841 atggttgccg tgccatcgct ctgcccggag atgccgagcc gtgcggggtc gacggtaccc
      901 gtctggatgg ccgcatcgat cgcgcgcgaa agagcggact ggacattgcg acgatcgcgc
      961 cagtcccgcg tgtcctggcg aaatgcctct gccatggatg tcgcgtgggc atcgacgggg
     1021 agttggggcg gtcgctggaa actcagcacg gcaaagccgt gagccgcgag cggcaggatg
     1081 ggataattgt cgccggtgcc tccgcgcagg aggccgcgcg aaacatattg gacgacaacc
     1141 agcggataac ggtgccggac gtcggcgtgc ggtggaagta cgaggtcggc aaaggtggga
     1201 ggcccctcat ccggcgccac aatcaatctg cggacggggc caagcgcgaa ctggaaggct
     1261 tgggggtttg ggtcgaacac tgtgcgcatt cggccggaat ggatgtcgac gaggacgatg
     1321 cgtcgcggct gtagcgatgc ctcgtcggcg cacaagagct gcgcgtcgac cgacgtgcag
     1381 tcgatcagca gatggtcggt ctggaagacg cgcctgggtt cgccgttccc gacatcccag
     1441 cggtagaagc cgaggtcgcc atagccggga ccctcgcgat gaaggaagaa aaggtggccg
     1501 ccaatccacc acaggccgat gatatggccg tcgcaagccg gggcgccgca ggcacggacg
     1561 gcgcccttgg tggtgcggac atggagtggt tcgggcgcat cataggctga gctgtcgggc
     1621 gcggtccaag cctggcgtcc gtcgcgggcg gatgcgaaga gatgtgcgga cggcggattg
     1681 cccggcgagc ggtcgccggc cagcagggcc gcgtccgagg cgctcgcggt gcccaggctg
     1741 ttcgttgcga gatcgagcgc gagcgcgacg cgcgggagcg gaccggtggg gaagggccga
     1801 tagccggcca tgggtacgac acggtcatcg atatggtaac cggatagtcc ttcccgatcg
     1861 atcgcctgct ccgcctcgac aagcatgggg cgcgccgaga ggatgagacg ccgtccgtcg
     1921 gcggaccatc gcaggtccgc cagatcgaga ttgcccggcg agacggcgcg agcatcgccg
     1981 ccgtcactat gggctatcca ggcggccgtc aggccatggt cgcgacgcag atacgccagc
     2041 cagcgtccgt ccggtgacca gcgcggagta acgagcgcgg gaatgcccga cggatattct
     2101 gcgattccgt agcgcgggta ggtgagcgcc ataaactcgc ctccccgatc gatgaccgat
     2161 ggcgcacgcg tggacagcga aacaacgata atggcctggc aatagctgtt ggagccggga
     2221 tcggcctggc gcaactggaa cgcgactttc cggtggtcgg gcgacaggct caggcctgcg
     2281 gcctcgacgc cgggctgtcc gatgtcgcgc acctgcatca ggtcctcgat tgtgatggac
     2341 tgccggcttg cagggacact gaccaccgac tgcggaagaa gacgctggca gcggtcgatt
     2401 ggatctgcat cggtcggatg aatggcggcg gcgaccgcca ggacgagccc gatcaccagc
     2461 gcttgccgac tgtcaggctg acggcccgcc ccaacgcgga atggttggtg gaatcgtaag
     2521 gcggcgcata gggggccgac acgatgatat ggggtggttt cgcgtcggtg aaattggtga
     2581 ctgaaaaggt cacgtcgaga ccacccggca gaccgcggtt atcaggcgca tggtagcgga
     2641 tcgtcgcgtc ggcggtggtc atgccgccga tcgcacgaac cggcgcgctg cgattgtcgg
     2701 tgagccttcc gatgacgttg acataggtgc tggcggtcag ggatttgctc gaccagatgg
     2761 cgccgaaacg tccgcgccag cgcggcggat tgaaaatcgt gccgctcaac gccgtgagcg
     2821 gttgttcgcc gctgagctgc tgccggctgt cgagataagt ggcgaggccg gtcagggcga
     2881 gcgagcggcc ggcggacaaa tccagcttat agtcgaccga caaatcgacg ccgttgatct
     2941 tttgacgcgc ggcgttgcga aagcgatcgt cgaacagaac ggcgacggca tttggattat
     3001 aggcgacgcc ggcgaaattc tcgaggccaa cggctgcatc ggagatcgcc gcttgctgga
     3061 gttcggcgga tggattggtc gtaataacgg atcggtagat tggatcgctc agcgcgccat
     3121 cgtcattgac cagtggctgt acgatgcggt tgcggtaggc aacatggaag tagctgacat
     3181 cgagcttgaa gcctgggacg aaggtcgggt ggagcgaaag ggtcgtggtc cagctcgtcg
     3241 ccttttcggg cttcaggtcg ggattgctgc cttcgcggag caggatagtc gagccggcag
     3301 gatagccgga tgcgccgaag aaaatgggat cgaccagaac ggcgcgggtg cccgagaatt
     3361 gttgatagag ggtcggcgcc ttgaacgatt tcccccagga ggccttgagc tccacgccgt
     3421 cgacgggcgc atagataagc ccgagcttgg gcgtcgcgac atgatcgacg tcgagatagc
     3481 tttcgtaccg aacggctgca ctggccgaca ggctgtggat gccgccgatg tcgagggcgg
     3541 gagagacgaa cggcagatag atttcgccat agccgtaggc ggcgttgcgc gaccggtcga
     3601 acacggtgtt gggttcgacg atattgccga atgcggtggc gagatagaga tcgagatggt
     3661 tggagcgata gccgccgccc aggactaccc gcgcgtcgcc cccgggaagg cggaacaggg
     3721 gcccctcggc cgagagttcg acgaccttca gggaattgtc atagtggccg gggaagcttt
     3781 ccaacacgct gccagcgaag aaataacggg tgtcgagccg cgtggaatcc ttgccatagg
     3841 tgccgctcag cacggcgcgc catcctgctg gtaaatcgaa ggtcagcctg ggcgaaaccg
     3901 agaaggcgcg aacgcgaggc gttgcgacgg tgccattggc ctggaacccg ccgctggtgg
     3961 taagcggcac gacgatcagt gtgcgtcgct cgttgtaacc gccgtcgacg tcgaagcgca
     4021 cccctggcgc gagtgcctga tgcccggcag ccaccaggtc cacatagcgt tgtctggggt
     4081 agagggttgc ggacggatcc atggcgcttg tgaaggaacg gtcgccggcg gtgacggcgc
     4141 tcgatcgtcc atattcggcc gaggccatga agccgccgcc cgaccagcgc ttgccggtga
     4201 gcgcatcata ttgctgctcg aaatcgcctc cacgggtcgt cgagccgatc cgggcgcttg
     4261 tgtagagccc gtcatagtcc tggcgaagaa tgacgttggc gacgccgccg acggcatccg
     4321 aaccgtagag cgccgatgcc ccgtcggcga cgacctcgat ccgatcgacc gcgaggagcg
     4381 gaatggacga gacatcgacc gactggatgg cggagtcgta gctggcgcga tgcccattga
     4441 tgagcgtcag cgtcgcgtcc gggccgaggc cgcggaggtt caagctggac gtcgagttga
     4501 cgttggtatc ggtgacccgg ctgcttccca tcaccacacc cggattctgg ccgccggggg
     4561 cgttctgggg aatatcgcgg atgacgtcgc ccaagttgtt ctggccggcg ttgcgcatgt
     4621 cttcgcgcgt gagcgtagtg acctgggagg caaccggcgc gcctcggatg cgcgagcccg
     4681 tcaccacgat cgcgctaggc tgctccgccg tgccggtcac ctcttcggcc ggcggcgttc
     4741 ctcgcccccg gatgatgata gctccatcgg acgtgtcggc cgaaaggctt gtcccttgga
     4801 tcaggcgctc gaccgcctcg tgcaccgtga aatcgccttc gagccgcggt gctgtgagcc
     4861 ccgccacgtc gcccggagag aacaggacct gcgcgttgga gtttgcggca agcgcgcgca
     4921 gcgcctgtgc catcggcagg gacggctgat ggtaatgtgc ccgcgacgcg gcctgcgccc
     4981 agcctaccga gggtaatgcg agagctgcac aggccgcgcc gtacagcagc ccgcggtgca
     5041 ggcgtgactt gatgttttcc atattcgatc cccccgacat ttatttcgtc gggtgggatc
     5101 gacgccctgg gaaaacgccc ccttaggtgc cgggcaaaaa atttagcctg agctgtcgag
     5161 aatgatccga tccggccgac tggtgtcgat cgacaagccg aagaggcgcg caagattacg
     5221 gctgagacca gccggttcgt cgatccggaa tcggcccgac acccgcaagc tgccgagcga
     5281 ggggtcgccg agcacaatcg gcgtcgtggc gtagccgttg gcgcgggcaa tcacgtccgc
     5341 cagcgaggca tcgtcgaagg cgatcatgcc cgtcggccag gcggtgtcct ggaggagccg
     5401 gg
//
LOCUS       DATGEM010000193         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_291761, whole genome shotgun sequence.
ACCESSION   DATGEM010000193 DATGEM010000000
VERSION     DATGEM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291761"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VKQ27_06195"
     CDS             complement(<1..413)
                     /locus_tag="VKQ27_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY88558.1"
                     /translation="MLRKEQNDLLTLTSAGTPMGEMFRRYWIPALLAEELPENDCAPV
                     RVRLLSEKLIAFRDSEGRYGLMDEFCAHRGVSLWFGRNEECGLRCPYHGWKYDVTGQC
                     VEIPSEPEKSGFRAMIRLRSYPLVARGGVLWTYMGP"
     gene            480..926
                     /locus_tag="VKQ27_06200"
     CDS             480..926
                     /locus_tag="VKQ27_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88559.1"
                     /translation="MLHTGGCHCGNITVALDLSLPPEEMPLRSCSCGFCRAHGTRTLS
                     DRNGRVEITATDWSQVERYRFGSHTADYILCRRCGIYIGAVCNTATGLRAVINTNCLQ
                     DRAAFTEAPTAPDYDGEATEARLARRSTNWMPAILHAPSPVAPLSA"
     gene            complement(883..1578)
                     /locus_tag="VKQ27_06205"
     CDS             complement(883..1578)
                     /locus_tag="VKQ27_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY88560.1"
                     /translation="MVRVAAVIPTLNEAETIAGTIAMLPRDVVDEVIVADSSSRDGTA
                     EIARAAGARVVSIAERGYGRACAAGVAVVGEGCGVILFLDGDGADRADLADMLVAPVR
                     AGTHDFVIGSRVRGEREPGSMSWHQVLAGRLAGWLMSALYRIHYTDMCAFRAISREAL
                     ATLDMRETTYGWNIEMQMKAARAGLRILELPVPYRCRAGGSSKVAGSLSGSVKAAWRI
                     TLTVARLAMARGG"
     gene            complement(1669..>2343)
                     /locus_tag="VKQ27_06210"
     CDS             complement(1669..>2343)
                     /locus_tag="VKQ27_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HLY88561.1"
                     /translation="DTGLDELRVSLDAADAKSFLAVRGKDFFHRIVRNVRSFTEFQRE
                     IGVEKPLVSLWLTGLKETIEQLPAFVKLAASMGVKEVHLQRLVYDESGYGLARPESSL
                     FETLREKETRSIEEAAALAGDLGVMLDASGATEPGLSLQRQEGWSACRRPWSLMYFTA
                     HGRALPCCIAPFSARGYESYTLGDATQQTLREIWNGPVYREFREALLTDAPPKPCAGC
                     GLRWSL"
BASE COUNT          437 a          953 c          562 g          391 t
ORIGIN      
        1 ggccccatat acgtccacag cacgccgccg cgcgccacca gcggatagga ccgcagcctg
       61 atcatcgcac ggaagccgct cttctccggc tccgagggga tctccacgca ctgccccgtc
      121 acatcgtatt tccagccgtg atacggacag cgcagcccgc actcttcgtt ccgcccgaac
      181 cacagcgaaa cgccgcgatg cgcgcagaac tcatccatca gcccatagcg tccctcgctg
      241 tcacggaacg cgatcagctt ctccgacagc agccgcaccc tcaccggcgc acaatcattt
      301 tccggcaatt cctccgccaa caacgccgga atccagtagc ggcggaacat ctcacccatc
      361 ggcgtgcccg cactggtaag ggtcagcaga tcattctgtt cctttcgcaa catcgccgtt
      421 tcctccgcga ttgccgccca aggctagtcc catgcgccgc aaccgcctag catcgcccca
      481 tgcttcacac tggcggctgc cactgcggca acatcacggt cgcgctcgac ctctcgctcc
      541 cacccgagga gatgccacta cgctcctgct cctgcggctt ctgtcgtgcc cacggcaccc
      601 gcacgctgtc cgatcgcaat ggccgagtgg aaataacggc gaccgactgg tcgcaggtcg
      661 agcgctaccg cttcggctcc cacaccgccg actacatcct ctgccgccgt tgcggtatat
      721 acatcggtgc cgtttgcaac actgcgaccg gtctgcgcgc cgtaatcaac accaactgct
      781 tgcaggaccg cgccgccttt accgaggcac ccaccgcccc cgactacgac ggcgaagcca
      841 cagaagcccg cctggctcgc cgctccacca actggatgcc cgctatcctc cacgcgccat
      901 cgccagtcgc gccactgtca gcgtgatccg ccacgccgcc ttcacactgc cgctcagcga
      961 ccccgcaacc tttgaactgc cccctgcccg gcagcgataa ggcaccggca gttccaggat
     1021 ccgcaggccc gcccgtgccg ccttcatctg catctcgatg ttccagccat atgtcgtctc
     1081 acgcatatcg agcgtggcca atgcctcgcg gctgatcgcc ctgaatgcgc acatatcggt
     1141 gtagtgaatg cgatacagcg ccgacatcag ccaccccgct aatcgccccg cgagcacctg
     1201 atgccagctc atcgaccccg gttcgcgctc gcctcgcacg cgggacccga tcacgaagtc
     1261 atgcgtcccc gcccgcaccg gtgccaccag catatccgcg agatctgcgc gatccgcgcc
     1321 atccccatcc aggaacagga tgaccccgca cccctcacct accaccgcga cgccagccgc
     1381 gcatgcccgc ccgtatccac gctctgcaat cgaaacaaca cgagcccccg ccgcacgcgc
     1441 gatctccgcc gtcccatccc tgctggaact gtcggcaact atcacctcat cgacgacatc
     1501 tcgaggcagc atcgcgatcg tcccagcgat cgtctccgcc tcattcagcg tcgggataac
     1561 cgccgcgacc ctcaccacct gtccgcgacc ctcaccccct gtcattgcga ccgaagcgaa
     1621 gcaatctcga gatcacttcg tcgccatcct cctcgtaccg acagccccct acagactcca
     1681 ccttagcccg cacccggcgc acggcttcgg cggtgcgtca gtcagcagcg cctcccgaaa
     1741 ctcccgatac acgggcccat tccagatctc ccgcagcgtc tgctgcgtcg catcgcccaa
     1801 ggtatagctc tcgtatccgc gcgcggagaa cggcgcgatg cagcacggca acgctcgccc
     1861 gtgcgcggtg aaatacatca gcgaccatgg ccgccggcac gccgaccatc cctcctgccg
     1921 ctgcaggctc agccccggct ctgtcgcgcc cgaagcatcc agcatcaccc ccaggtcacc
     1981 ggccaacgcc gccgcttcct cgatgctccg cgtctccttt tcccgcagcg tctcgaacag
     2041 cgaagactcc ggccgggcca acccatagcc gctttcatcg tacaccagcc gctgcaggtg
     2101 tacctccttc acccccatcg acgctgccag cttcacgaac gccggcaact gctcgatcgt
     2161 ctccttcagc cctgtcagcc acagcgacac cagtggtttc tcaactccga tctcacgctg
     2221 gaactcggtg aaacttctta cattccgcac gatccgatgg aaaaagtcct tgccccgcac
     2281 agcgagaaac gattttgcat ccgccgcgtc cagcgacacg cgcagctcat cgagcccggt
     2341 gtc
//
LOCUS       DATGEM010000194         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_403745, whole genome shotgun sequence.
ACCESSION   DATGEM010000194 DATGEM010000000
VERSION     DATGEM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403745"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VKQ27_06215"
     CDS             <1..264
                     /locus_tag="VKQ27_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HLY88562.1"
                     /translation="LLTSMDRDGTGLGFDLDLLQAVCGAVRLPVIASGGVGTLQHFVE
                     GAQAGATGLLAASVFHFGTFTIAQVKAALNAAGLPVRVPRKAA"
     gene            275..760
                     /locus_tag="VKQ27_06220"
     CDS             275..760
                     /locus_tag="VKQ27_06220"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HLY88563.1"
                     /translation="MGKSVKQKPPAKPKKQRKPKKRLVTAKDTEGPRQPPPLAIRPAE
                     EVLHRLWSVVMSRRDADPAASHSARLLSRGTAKVAQKFGEEAVECLIEAVAGNQAAVI
                     AESADVLYHLIVLWVASGITPNEVWDELKRREGISGIAEKASRARNLPVAFGLRTTKI
                     P"
     gene            780..1154
                     /locus_tag="VKQ27_06225"
     CDS             780..1154
                     /locus_tag="VKQ27_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HLY88564.1"
                     /translation="MAVSGLPPYDSGNIFARILRGEIPCKKVYEDEWALAFHDINPQA
                     PTHVLLIPKGNYVSFADFSASASAEEIAGFMRVVGKVAKDLGLEAQGYRLLANMGEHS
                     GQEVPHFHVHLFAGKPLGRMLV"
     gene            1199..1882
                     /gene="aat"
                     /locus_tag="VKQ27_06230"
     CDS             1199..1882
                     /gene="aat"
                     /locus_tag="VKQ27_06230"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HLY88565.1"
                     /translation="MSRRTVEITTDLMLRAYRHGLFPMAETRRGDRLYWLDPKKRGIL
                     PLDRFHLPRRLARTVVSDAFTVTVDEDFAGTIAGCATAAPGREDTWINPQIERLFGDL
                     HEQGHAHSVESRIEGHLVGGLYGVAIGGVFFGESMFSFARDASKVALVHLVVRLRLGG
                     FELLDTQFVTAHLSQFGAEELPREEYKARLARALTAPAVFPVEADAADVAGEIRRMGE
                     RQAAVAGGH"
     gene            1879..2436
                     /locus_tag="VKQ27_06235"
     CDS             1879..2436
                     /locus_tag="VKQ27_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88566.1"
                     /translation="MRRLAVLALLLCWLAPGAWADEPLLRPARDVDVTYRATAGAQVL
                     QQRVRWSVAAQSMRIDLPTTGAYVIIDYLTRRMSVVHDQEKSVVEMAAPAGMASVPGS
                     VPDGKFVRHGDAVVNGVGCSEWDTTDRTGQQADVCITSDGVMLQASAQGRTLVSAVKL
                     QYEPQDPGLFRVPADYTRKVLGAGR"
     gene            2433..3332
                     /locus_tag="VKQ27_06240"
     CDS             2433..3332
                     /locus_tag="VKQ27_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HLY88567.1"
                     /translation="MIVVPWRDRRGKFLPLKAAVLAASFVPGAVLAYWWAAGMLSSRP
                     VTDVIHGAGDWAIRYILISLAITPAARVLNWPRLLLVRRTVGLTALAYVLAHLTLYAL
                     DQNFRLGFVVSEIVHRFYLTIGFVALVGLGVLGATSTNGWIARLGRWWKRIHWGIYVI
                     GVVALVHYFIQSKANVSEPVFFSGLFVWLMLWRAVPMSWQRNVAVHLGLMVVAAVGAA
                     WIEFAWYAIATGINPWRVLAASETLRFGLRPAHYVALMGLGVTALAAVRRYGPMVWSR
                     GRWPVAGGRLKAGHDRPAVTTPP"
BASE COUNT          524 a          944 c         1304 g          594 t
ORIGIN      
        1 ctgctcacga gcatggaccg tgacggcacc ggcctggggt tcgacctcga tctgctgcag
       61 gcggtctgtg gcgcggtccg gctgccggtg atcgcgtcgg gcggcgttgg cacgctgcag
      121 catttcgtgg agggcgctca ggcgggcgct accgggctgc tcgccgcgag tgtgttccac
      181 ttcggcacgt ttacgattgc gcaggtgaaa gccgcattga atgcggctgg gttgcccgtg
      241 cgtgtaccgc gcaaggcggc ctgaggaccg tcgcatggga aagtccgtca agcaaaagcc
      301 gcctgcgaag ccgaagaagc agcgcaagcc gaagaagcgc ctggtcaccg cgaaggatac
      361 ggagggcccg cgccagccgc cgccgctcgc catccggccc gcggaggagg tgctccatcg
      421 gctgtggtcg gtggtgatga gccggcgcga cgccgatccg gcggcgagcc actcggcaag
      481 gctgctgtcg cgcggcacgg ccaaggtggc gcagaaattt ggcgaggaag cggtcgagtg
      541 cctgatcgag gcggtcgccg gcaatcaggc tgcggtgata gccgagagcg ccgacgtgct
      601 ctaccatctg atcgtgctgt gggtcgcgag cgggatcacg ccgaacgagg tgtgggacga
      661 gctgaagcgc cgcgagggga tcagcgggat tgccgagaaa gcctcgcggg cccgcaatct
      721 tccagttgcg ttcgggctgc ggacgacgaa gattccctag cgcacaggga gtatctgtca
      781 tggctgtcag cggcctgccg ccatatgatt ccggcaacat cttcgcccgc atcctgcgtg
      841 gcgagatccc gtgcaagaag gtctacgagg acgagtgggc gctggcgttc cacgacatca
      901 atccgcaggc gccgacacat gtgctgttga tccccaaggg aaattacgtg tcattcgccg
      961 atttcagcgc cagcgcctcg gccgaggaga tcgccgggtt catgcgtgtt gtaggcaagg
     1021 tggcgaagga cctgggactg gaggcgcagg gctaccggct gctcgccaac atgggcgagc
     1081 acagcgggca ggaggtgcca cacttccatg ttcacctgtt cgcgggaaag ccgctgggac
     1141 ggatgctggt ctagcgaacg gccttcccct gagcacggcg tccgccgcac aatagggcat
     1201 gtcccggcgc acggttgaga tcacgacgga tctgatgctg cgggcctatc ggcatgggct
     1261 gttccccatg gcggagacgc ggcgtggcga ccggctgtac tggctggatc cgaaaaagcg
     1321 cgggatcctg ccgctcgacc ggttccacct gccgcggcgg ctggcgcgca ccgtggtgtc
     1381 cgatgcgttc accgtgacgg tggatgagga ctttgccggc acaattgccg gttgcgcgac
     1441 cgcggcaccg gggcgggagg acacctggat caacccgcag atcgagcggc tgttcggtga
     1501 cctgcacgag caggggcacg cgcattcggt ggagagccgc atcgaggggc acctggttgg
     1561 cgggctgtat ggcgtggcca tcggaggagt gttcttcggc gagagcatgt tcagcttcgc
     1621 gcgcgatgcc tcgaaggtgg cgctggtgca cctggtggtg cggcttcggc tggggggatt
     1681 cgagcttctc gacacacagt ttgtcaccgc gcacctgtcg cagttcggtg ccgaggagct
     1741 gcctcgggaa gaatacaagg cacggttggc gcgcgcgctc actgcgccgg cggtgtttcc
     1801 ggtcgaggcc gatgcggcgg acgtcgcagg cgagatacga cggatggggg aacgccaggc
     1861 agcggttgca ggcgggcatt gaggcggctg gccgtcctgg cgctgctgct atgctggctg
     1921 gcgccggggg cgtgggctga tgagccgctg ctgcggccgg cacgtgacgt cgatgttacc
     1981 tatcgcgcga cagcaggggc acaggtgctg cagcagcgcg tgcggtggtc cgtggcggcg
     2041 cagtcgatgc ggatcgatct gcccaccact ggcgcatatg taatcatcga ctacctgacg
     2101 agacgaatga gcgtggtgca cgatcaggag aaatcggtgg tcgaaatggc agctccggcc
     2161 ggcatggcca gtgttcccgg cagcgtgcca gacggaaagt tcgtccggca cggtgacgcg
     2221 gtggtgaatg gggtcggctg tagcgaatgg gatacaacgg atcggaccgg gcagcaggcg
     2281 gacgtctgca tcacgagcga cggcgtgatg ctgcaggcga gcgcgcaggg acggacacta
     2341 gtgagtgcgg tgaagttgca atacgagccg caggatccgg ggctgttccg ggtgccggct
     2401 gattatacgc ggaaggtgct gggggcgggg cgatgatcgt cgttccgtgg cgtgaccggc
     2461 gcggcaaatt cctgccactg aaggcggcgg tgctggcggc gagcttcgtt ccgggggcgg
     2521 tgctcgctta ctggtgggct gctgggatgt tgagcagccg gccggtgaca gacgtgatcc
     2581 acggggccgg ggattgggcg atccgctaca tcctgatctc gctggcgatc acgccggcgg
     2641 cgcgggtgct gaactggccc cggctgttgc tggtgcggcg gacggtaggg ctgaccgcgc
     2701 ttgcctacgt gctggcgcat ctgacgctgt atgcgctgga ccagaacttc cggctcgggt
     2761 tcgtggtgtc ggagatcgtg caccggttct acctgacgat cgggttcgtg gcgctggtgg
     2821 gattgggggt actgggggcg acctcgacca acggttggat cgcgcgactg ggacggtggt
     2881 ggaagcgtat ccactggggg atatacgtca tcggcgtggt cgcgctggtg cactatttca
     2941 tccagtcgaa ggcgaacgtg tcggagccgg tctttttctc ggggctgttc gtgtggctga
     3001 tgctgtggcg cgcggtgccg atgtcgtggc agcggaatgt ggcggtgcac ctggggctga
     3061 tggtggtggc ggcggtcggc gcggcatgga tcgagttcgc atggtacgcg atcgcgacgg
     3121 ggatcaatcc gtggcgcgtg ctggcggcga gcgagacgct gcggttcggc ttgcggccgg
     3181 cgcattacgt ggcgctgatg gggttgggag ttaccgcgct ggctgcagtg cggcggtatg
     3241 ggccgatggt gtggtcgcgg ggcaggtggc cggtggccgg tggccggctc aaggccggcc
     3301 atgacaggcc ggccgtgacg acaccacctt aggagccgcc gaagccgtcg agagtgctgg
     3361 cgcgga
//
LOCUS       DATGEM010000195         7054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1085434, whole genome shotgun sequence.
ACCESSION   DATGEM010000195 DATGEM010000000
VERSION     DATGEM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085434"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VKQ27_06245"
     CDS             complement(<1..1274)
                     /locus_tag="VKQ27_06245"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLY88568.1"
                     /translation="MIGEPVAAAGRPWSARTVPATLLDRLALCVEALVVVTIFANILL
                     TFTNAMVRYLTNQDFPWASDTWAVLISIITFLGAPAYFRRGAGMAYTALIDQSHGIRK
                     QMLQASGLAIFLGVCLVALAAYPSFLASQLTQTLQVLGISSAFVAIWLGVGMVLFCIF
                     TIEKLAAFERRAVATGVAVGVVIALATLALRWAFDQDLIEIDPFIPIVPALVIGFLTG
                     APIAGILALAGLLYFVITGEAPITTIASAMQYGVSSFVLLAVPFFMVAGTLMEVTGMA
                     RRMIDMVQEWVGHWTGGLLVAEVVAMYVFSGLSGSKAADIATVGGVMKTPLRHYGYPP
                     TESVAVLAASAAMGETIPPSLMLLVLGSITSLSVGSLFLAGLLPAAILALVLIAVVIV
                     RSHMRGYVKGPPFRIGRALLSTPPAIPAFLVPV"
     gene            1418..2287
                     /locus_tag="VKQ27_06250"
     CDS             1418..2287
                     /locus_tag="VKQ27_06250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLY88569.1"
                     /translation="MTEITGLQRYPFARRSFVMTSLISGLTLATTRVEAQAIHTDTEG
                     LNAGEVQIPVQGGNMPAYCARPAKGTSFPIILVNEEIFGVHDYIQDVCRRFAKLGYLA
                     IAPEIYFRNPDFPKMTDAQQIMREVIFKTPDAEIWSDLDATLAWASHNHGDSSRMGEI
                     GFCFGGRNTWEYAAHNPHLKAAVAFYGHIKNPTSDIKPKTVLDIADQIHCPLLGLYGG
                     QDPINPVDLVHQAEAKAKGAHKIVEIVIYPDAPHGFHADYRPSYREADAKDAWNRALA
                     WFKRYGVAPKGAS"
     gene            complement(2257..3492)
                     /locus_tag="VKQ27_06255"
     CDS             complement(2257..3492)
                     /locus_tag="VKQ27_06255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HLY88570.1"
                     /translation="MRAHLIDLLRRADWLTPDRVVAYSGVLLVMELLILVFLALWQHG
                     VFAAAVHSPASDFASFYAAGKLTLAGTPQLAYDQAAHYLAQKQATGTDAPHQYFFYPP
                     VYLLLCAPLAGLPYLVAFGVFQVVTLVVFIGTMRSLLRLHGAAWLLPVLAFPSVFWTI
                     GLGQNAFLNAALFGGFTLLLDRKPVTAGMLLGCLCYKPHFGLLAPVALLAGRQWRVIV
                     GAAVAVAACVAASVSLFGWGTWGAYLAELSHSDQVYSTGTIDFAGIVTPFGAARLLGV
                     PPNAAYAVQAVVSVGMVALIAAVWLRVTSRNLRAATLVSATLLAAPLVLLYDQLLLLI
                     AIGWLLCEARQTGLLAWERIVLLAIYPLALVSLPLSHSTHLPLGLVISVAVLGLCLRR
                     ASRSLPARRSYDAPLGATP"
     gene            complement(3535..4029)
                     /locus_tag="VKQ27_06260"
     CDS             complement(3535..4029)
                     /locus_tag="VKQ27_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HLY88571.1"
                     /translation="MAVEKQPYREAMARLGAAVNVITTDGPGGRAGFTASAVTSVTDT
                     PPTLLVCANRTNDSYPAMKANSVLCVNTLAGAHEALSPVFAGMTDHAMEARFDGATWH
                     TLVTGSPVLDGALVAFDCRIEQIVEVGTHDIFICAVDAVEVGTVTEGLIYYARGYHRV
                     VHTG"
     gene            complement(4123..4383)
                     /locus_tag="VKQ27_06265"
     CDS             complement(4123..4383)
                     /locus_tag="VKQ27_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HLY88572.1"
                     /translation="MRYGKLAAVSLGIALALSACSNPYDPGQRALGGAAIGAGAGAAI
                     GGLAGGWHGAAIGALAGGALGAVTGAVTTPHPPSRGYGGPQY"
     gene            5376..5960
                     /locus_tag="VKQ27_06270"
     CDS             5376..5960
                     /locus_tag="VKQ27_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88573.1"
                     /translation="MRRVGVVGIGPPRHVAVLPGVIAARIRIATVAARVTTIVGGRMM
                     AIIAIGMPAAVVVEAARIVMVEVAAVILIEVAAVILIEAARIVVVEAAAVIAVEPPVA
                     AIAGIRIMVPPHHVPMRVLPLRIQLVVICAHGGAVVPGICVAGRSHLPEHLANAATAL
                     GLTGHRGSSAIWRRLILPYGCRSGSCIAGLSLTR"
     gene            complement(6142..6660)
                     /locus_tag="VKQ27_06275"
     CDS             complement(6142..6660)
                     /locus_tag="VKQ27_06275"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HLY88574.1"
                     /translation="MSPICHIAVIMQQVRGRASPAACRLAGARGRFGAVLAIGSMLLL
                     AGCAAPAGLAIFSAGTGVAMGTGVDYTLNGIAYKTFVTPLPRARQATLASLNRMGMKV
                     VEDKKTDSGWTITATAEGRDIDIDLQRLTPRTTRMRVVANTGPFFKDRATEAAIIDEA
                     ADTLDRQGGLRG"
     gene            6852..>7054
                     /locus_tag="VKQ27_06280"
     CDS             6852..>7054
                     /locus_tag="VKQ27_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88575.1"
                     /translation="MAIPLTRLTILVTALTLGGCSDVDRAYYTVSNTVHSAFYDLRHT
                     NSARPAHSTSQAPAPPGNYNDASV"
BASE COUNT         1375 a         2344 c         2173 g         1162 t
ORIGIN      
        1 accggcacga ggaacgccgg aatcgccggc ggcgtgctga gcagtgcgcg cccgatccgg
       61 aacggcggcc ccttcacgta accacgcatg tgactacgga cgatgaccac cgcgatcagc
      121 accaacgcga ggatcgccgc cggcagcagt cctgcaagga acagcgagcc aaccgacagg
      181 ctggtgatag atccaagcac caggagcatc agcgagggcg ggatggtctc gcccatcgcc
      241 gcgctcgccg ccagcaccgc gacggactcc gtcggcggat agccatagtg ccgcagcggc
      301 gttttcatca cgccgccaac tgtcgcgata tcggccgcct tcgatccgct gagccccgag
      361 aacacgtaca tcgcaaccac ttcggcgacc agcaggccgc cggtccaatg ccccacccac
      421 tcctgaacca tgtcgatcat ccggcgcgcc atgccggtga cttccatcag cgtacccgcg
      481 accatgaaga agggcaccgc aagcaacacg aagctgctga ccccgtactg catggcggat
      541 gcaatagtgg tgatcggcgc ctcgccggtg atgacaaagt acagcaggcc ggcaagtgcc
      601 aggatgccgg caatcggcgc gccggtcagg aatccgatga ccagcgccgg cacgatgggg
      661 atgaagggat cgatttcgat caggtcctgg tcaaacgccc accgcaatgc gagcgtcgcc
      721 aacgcgatca ccacgccaac ggccacacca gtggccacgg cccgccgctc gaatgcagcc
      781 agcttctcga tagtgaaaat gcagaacagc accatcccga cgccaagcca gatcgccacg
      841 aatgcggaac tgatgccaag aacctgcagg gtctgggtga gttggctcgc aaggaacgac
      901 ggatacgcag ccagcgcgac caggcagacg ccgaggaaga tcgccagccc tgatgcctgc
      961 agcatctgtt tgcgaatgcc gtgggactgg tcgatcaagg ccgtgtaggc catgcccgcg
     1021 ccgcgccgaa aatacgccgg ggcgccgagg aaggtgatga tggagataag gacagcccag
     1081 gtgtcggacg cccaggggaa atcctgattg gtcaggtagc ggaccatggc gttggtgaac
     1141 gtgagcagga tattggcaaa aatagtgaca acaaccagcg cctcgacgca cagcgccagc
     1201 cggtccagca gagtcgcggg cacagtgcgt gcggaccacg gcctgccagc cgcggcgact
     1261 ggctcgccga tcaactcccg tgccctccca ggtcattcgt ggaatgccat gctgccgtgc
     1321 cctcgtgagt gcgtccagca ggcccattgc ggaaatccac aatctcccca agtctctgcc
     1381 gccgcacgtc agggggagga agagcacgat gaacgaaatg accgagatca cgggcctgca
     1441 gcgttaccca ttcgcgcgcc gcagtttcgt gatgactagt ctgatcagcg gcctgaccct
     1501 cgcgaccact cgggtagagg cccaggcaat ccacaccgac acggaaggcc tgaatgccgg
     1561 cgaggtacaa atcccggtac agggcggcaa catgccggcc tattgtgccc gtcctgccaa
     1621 gggcacctca ttccccataa tcctggtgaa cgaggaaatc tttggcgttc atgactacat
     1681 ccaggatgtc tgccgccggt ttgcgaagct cggttacctg gcaatcgcgc ccgagattta
     1741 cttccgcaat cccgacttcc caaagatgac cgacgcgcaa cagatcatgc gagaggtcat
     1801 cttcaaaaca ccggacgccg aaatctggtc agatctcgat gccacgctgg cttgggcctc
     1861 gcataaccac ggcgactcca gccgcatggg cgagatcggc ttctgcttcg gcggccgcaa
     1921 tacctgggag tacgccgcgc acaatcccca cttgaaagcg gctgtcgcct tctatggcca
     1981 catcaagaac cccacgtcgg acatcaagcc caagaccgtg ctggatattg ctgaccagat
     2041 ccattgcccg ctgcttgggc tatacggcgg ccaggatccc attaatccgg tcgatctggt
     2101 ccaccaggcc gaagccaagg cgaagggcgc gcacaagatc gttgaaatcg tcatctaccc
     2161 cgatgcgccg cacgggtttc acgctgacta tcggccgagc tatcgagaag cggatgcgaa
     2221 ggatgcgtgg aaccgcgccc tcgcctggtt caagcgctac ggcgtggctc ccaagggcgc
     2281 gtcgtaagaa cgtcgcgccg gcaacgaccg cgaggcccga cgaaggcaca ggccaagcac
     2341 cgcgacactg atcaccagcc cgagcggcag gtgggtggag tggctcagag gcaggctgac
     2401 cagggcaagc gggtagatcg ccaacaggac gatccgctcc cacgccaaca ggccagtttg
     2461 ccgcgcctcg cacagcagcc atccgatcgc gatcagcaac agcagttggt cgtagagcaa
     2521 caccagtggc gcagccaaca acgtggcgga cacgagagtg gctgcgcgca ggttccggct
     2581 ggtgacacgg agccagactg ccgcgatcag cgccaccatg cctaccgaga caaccgcctg
     2641 caccgcatag gccgcattcg gcggcacacc cagcagccgt gcggcgccaa atggcgtgac
     2701 gatgccggca aagtcgatcg tgccggtcga atacacctga tccgaatgcg ataattctgc
     2761 cagataggct ccccaggtgc cccaaccgaa caacgagacc gaggcggcga cgcacgctgc
     2821 gaccgcgacc gcggcgccga cgatcacccg ccactgccgg cctgccagca acgccaccgg
     2881 tgccagcaga ccgaaatgcg gcttgtagca caggcaaccc agcagcatac cggcggtcac
     2941 tggtttgcgg tccagcagca acgtgaagcc accaaacagt gctgcattca ggaaggcgtt
     3001 ctgtccaagc ccgattgtcc agaacaccga ggggaacgcc agcacgggca acagccaggc
     3061 cgcaccgtgc aatcgcagca gcgaccgcat cgtcccaata aaaactacca gcgtgacgac
     3121 ctgaaaaacc ccgaatgcca ccagataagg caacccggcg agcggtgcgc agagcagcaa
     3181 atacacgggc ggatagaaga agtactgatg tggtgcgtcg gtgccggtag cctgcttctg
     3241 cgccagataa tgagccgcct gatcataggc gagctgcggc gtgcccgcta gcgtcagttt
     3301 tcccgccgcg tagaaactcg cgaagtctga ggccggtgaa tggaccgcag cggcgaacac
     3361 cccgtgctgc caaagtgcca ggaagaccag gatcagcagt tccatgacga gcagcacgcc
     3421 actgtaggca acgacgcgat cgggggtcag ccaatcggcg cgcctgagca ggtcgatcag
     3481 atgggctcgc atcttccagc actatacccg gccttcttga gaaagcgttt cagttcagcc
     3541 ggtatggacc acgcgatgat atccgcgagc atagtatatc agaccctcgg tcaccgtacc
     3601 cacctcgacc gcgtccacgg cgcagatgaa gatgtcatgg gtgccgactt cgacgatctg
     3661 ctcgatgcgg cagtcgaagg cgaccagcgc gccgtccagc accggcgagc cagtcaccaa
     3721 agtgtgccag gtggctccat cgaaacgggc ctccatcgcg tggtcggtca tgcctgcgaa
     3781 caccggcgac agcgcctcat gcgcgccggc cagcgtgttc acgcacagca cgctgttggc
     3841 cttcatcgca ggataggaat cattcgtgcg attggcgcag accagcaggg tggggggtgt
     3901 atcggtgaca ctggtgaccg ccgacgcggt aaatcccgca cgtccgcccg gtccatcggt
     3961 ggtgatcacg ttcaccgccg cgcccagacg cgccatcgcc tcgcgatacg gttgcttttc
     4021 gactgccacg gcttgctcca tcccctgggc ttgcgtccat aactatgacg cgatcggctg
     4081 acggaggaca gaggcgcggc ggtcagcagt atcagcgcga gatcagtact gcggcccacc
     4141 atagccacgt gacggcgggt gcggcgtggt aacagcgcca gttaccgcgc ctaatgcgcc
     4201 gcccgccaat gcgccgatcg cggcgccatg ccagcctccc gcgaggccgc cgatcgccgc
     4261 acccgcgccc gcaccaatcg cggccccgcc gagcgctcgc tgtccgggat cgtagggatt
     4321 gctgcaggcg gacaaagcca aagcaatgcc gagcgatact gctgccaact ttccataacg
     4381 cacgatcaac cactccttac ggctgggacg cgttgcatga gcgtcccgga tttctcctgc
     4441 tcgtcatccg cctagatgcg ggagccgaaa tgcgaatgcg tggtgctgcc cgcggacgtt
     4501 gtcgagctgc tgctaccagc cctgacaagg gtgcccgcgc tgctcgcact gccaccaagg
     4561 ccggtcgtga tcgtcgtgtg gtgtacgctg gaaatggcgg ctgtatgaga tcccgctgcg
     4621 ccctgcccag cattgctggc ggttaccagg ccatgtgcgg cgcccgacgt gctgcccagg
     4681 cccgtggtga acgacgaact ccgtgcagct gcagtcgtga atgtgcgggt tccaccagcc
     4741 gtcgcactgc tgtggacggc gcccgcggtc ccccccagac cggtggtgaa cccggaatta
     4801 tgtgcggacg cagtcgcgaa ggtgtggctc ccactgctcg tcgcctgact tgcgttcccc
     4861 gcggtgacca tgctgtgggc agcgcccgct gtcccgccta gacccgtggt tatggtggtg
     4921 ccttgtggcc ctgacgccgc gagggccccg ttgccgccgg tcactgcatg gttcgtattg
     4981 ctggcggtcg tcaccgtgtg gtccgtctga acgccagata cgtggctgcc actggcaaac
     5041 gtttgggcgt tgttgcctgc ggtcacagcc gcgcggccgc taccgctggt caccccagag
     5101 gaagtcgact tcgccaggtt ggtatgagcc ggggtcgccg aggcgacacc tgacgcgccg
     5161 agcgcggcgc cctgcgtggt ccccgatgcc gtggttgttt caccaccttt gcccgattgc
     5221 gggctcctac cggttggcga tccgctcgat gtcggcaagc tgccgccggc cgctgtggtc
     5281 gcagtgccgg tccccgcgcc tgatcggcca cgcgcccccg cgccgccaga ccctgtagag
     5341 cctccgccac ccgctgcagg ggagccgccg ttctgttgcg gcgcgtgggt gtcgtcggga
     5401 tcggtccgcc gcgtcacgtc gcggtcctgc ctggtgttat cgccgcgcgc atccggatcg
     5461 cgactgtcgc ggctcgcgta acgaccatcg tcggaggccg catgatggcc atcatcgcga
     5521 tcggcatgcc ggctgccgtc gtggtcgaag cggcgcgcat cgtcatggtc gaagtggcgg
     5581 ctgtcatcct gatcgaagtg gcggctgtca tcctgatcga agcggcgcgc atcgtcgtag
     5641 tcgaagcggc ggccgtcatc gcggtcgaac caccggtggc cgccatcgcg ggcatccgca
     5701 tcatggtgcc accacatcat gtcccgatgc gcgtcctgcc gctccgcatt cagctcgtgg
     5761 tgatatgcgc tcacggcggc gccgtggttc ctggcatctg tgtcgccggc cgttcgcatt
     5821 tgccggaaca cctcgccaat gccgcgactg cgctcggcct caccggacat cgcggcagct
     5881 ctgcgatttg gcgtcgcctg atcctcccat acgggtgccg tagcggcagc tgcattgccg
     5941 gtctgtcgct gaccagatga ccagtggttg gcggccgtag atacactcgc ggggtgccag
     6001 gcggagtaat cggatgcctc actttgcgcc catgcgtcgg gcatgagcaa aaagctgccc
     6061 aacgcaccgt agacgatggc gtttcgcatc attgcctcct gattttcttt tgaccaaaga
     6121 cctgcatgcg cccgtcacct gctaaccgcg caagccacct tgccgatcga gcgtgtcggc
     6181 ggcttcgtcg atgatcgccg cctctgtcgc tcgatccttg aagaacggac cggtgttggc
     6241 gaccacgcgc atgcgggtcg tccggggcgt cagtcgctgc agatcgatgt cgatgtcacg
     6301 accttcggcg gtagccgtga tcgtccaacc tgaatcagtc ttcttgtcct cgacaacctt
     6361 catgcccatg cggttcaggc tggcgagggt cgcctgtcgg gctcgcggca acggcgtgac
     6421 gaacgtcttg taggcgatac cattcagcgt gtagtccaca ccggtgccca ttgcgacacc
     6481 ggttccggcg ctgaaaatcg ccaggcccgc tggcgccgcg cagcccgcca acagcaacat
     6541 cgaaccgatc gcgaggacag caccgaagcg gccccgggcg ccggcaagcc ggcaagccgc
     6601 cggactggca cgaccccgga cttgctgcat gatcacggcg atgtgacaaa tggggctcat
     6661 gatcaacctc aatccatccg acccgtgaaa aggctttggt gatagtgatt ggcttccagg
     6721 aacgaagagc tacgggccgg gatgcggcaa ggcaatccgt gtccctgctt tgtcacaggc
     6781 tgtgacgtga tgggacaaat gcggcagtga caggcaaggg aacgtctgct ggccatgggc
     6841 ggcgtgacgc aatggcgatc ccattgacgc gccttaccat tctcgtcacc gccctcacgc
     6901 tcggaggctg ctcggacgtt gatcgcgcct actacacggt ttcgaacacc gtgcactcag
     6961 cattctatga tctgcgccac acgaattccg cccgtccggc gcattcaacc tcgcaagcgc
     7021 cggcgccgcc agggaactac aacgacgcat ccgt
//
LOCUS       DATGEM010000196         8344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_628741, whole genome shotgun sequence.
ACCESSION   DATGEM010000196 DATGEM010000000
VERSION     DATGEM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628741"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VKQ27_06285"
     CDS             <1..240
                     /locus_tag="VKQ27_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88576.1"
                     /translation="PRASIPELVRADPALTAALALLHVAARGIGSHRLVAAPAVIARI
                     EARPDWIEALARRIGGSIGLRADPALAISAGHVEP"
     gene            227..448
                     /gene="yacG"
                     /locus_tag="VKQ27_06290"
     CDS             227..448
                     /gene="yacG"
                     /locus_tag="VKQ27_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HLY88577.1"
                     /translation="MSNPDPRRDPRRRRADACPICGKPSAAPHTPFCGQGCRDRDLLQ
                     WLGEGYRVQGRPMELDGEEGLDSAANLPL"
     gene            477..552
                     /locus_tag="VKQ27_06295"
     tRNA            477..552
                     /locus_tag="VKQ27_06295"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:510..512,aa:Phe,seq:gaa)
     gene            714..1925
                     /locus_tag="VKQ27_06300"
     CDS             714..1925
                     /locus_tag="VKQ27_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HLY88578.1"
                     /translation="MLTDAAIHRIKARDKPYKVSDMHGLYLLVRPDGARYWRMDYRHA
                     DKRGTVALGVYPAVSLKEAREKRENARKLLDKGINPSSYRKLTRAVGSISSGVTFKAV
                     ADEWLNKLEAERRSAATLDKMRWLLRFAEPLIGGRPIGEITAPELLTVLRTVEVRGRY
                     ETARRLRGTCGSIIRYAIATGRAERDVTTDLHGALITPRVKHHASITEPNGVGELLRA
                     IEDYRGQPTVAIALRLAPHLFVRPGELRSAEWAEFDLAAAVWTIPGDKMKMGRSHKVP
                     LSRQVQALLKDLAPISGRSKFLFPGIRSNDRAISENTINAALRRLGYGKTEMTGHGFR
                     AMASSLLNESRKWHPDAIERQLAHVESNDVRRTYLRGEHWAERVKMMQWWSDYLDQLR
                     SRGKIVRRKFG"
     gene            2016..4217
                     /locus_tag="VKQ27_06305"
     CDS             2016..4217
                     /locus_tag="VKQ27_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88579.1"
                     /translation="MLHLAEALHEFVAMDHEAAPGRIGTAMRAFLSGTLLLAGLVSGS
                     RALADPAPFDLAGPALRVTVTHQGVTLPIAEVPQLAAGDSVKVRADLPDDQSAHYVLV
                     AAFLRGSTNPPPDKWFYQAESWTKKGRKGLSLTVPDGAQQIVLFLAPATGGDYPTLRN
                     TVQGRPGAFVRAAQDLAQASLDRSRLDTYLTAVRKTNPGDPNRLDRITPLLARTLQVK
                     VNTDCLTKTPELQAACLLQDQESLVLNDGHSNAITEAVAGPGADLALQLSATPQGGLG
                     YYSPYIAAIRDIIGIFSSIHTAKYQYIPALATLSNDRMGLVLNAPPSFHNPKSVLVTA
                     LPIVAPVHVPPLQVANQAPSLCAQAPELLLPISGAPLIYATRYAHDLSLRVRLPNGRS
                     IDLPATPDAERGGLVIQTQGKLPADLKVPLDASLHGIWGFQPFDGPSVRLQPAQPGQW
                     RLPPGAATAPNATVTLTGGTAACVSGVSIQSGGTAQPVSWTAAKSDEIRVTLPAIDDK
                     SGATTLEITGPAGLPPDRLTIAGPPPPGHFGTTLIARSMQRPPATAPVAIKLGSDSEV
                     PSDGTLAFSLRANKGARFTGREKVEVATASGDATATLSPSNGLTLADPQVAIAIIQPA
                     KALGASAFGPIRARVSRDGVEGDWLPLGTLVRLPSLHQLTCPPDASMACELTGDGLFL
                     ISALSATPGFEHPTAVPEGYPGNAIQVPHPVDGKLYVRLHDDPDTVSNITG"
     gene            complement(4642..4899)
                     /locus_tag="VKQ27_06310"
     CDS             complement(4642..4899)
                     /locus_tag="VKQ27_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88580.1"
                     /translation="MRERKPIDGTDIESNLVEIRRYLDTRLRLTGQDEGASDPREPSA
                     WETYRYAEYSDGRRPMFAPTDLILVVLVLILLGLLAVFLTY"
     gene            complement(5376..6578)
                     /locus_tag="VKQ27_06315"
     CDS             complement(5376..6578)
                     /locus_tag="VKQ27_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HLY88581.1"
                     /translation="MLTRLASAPPDPLWAITAAFEADSRPDKMDLGLGIYRDDSGRTP
                     VLASVLTVERQLAAFAPSKAYRPLAGNAAFNAGVTRLLLVGDASRDDRTVTLQTVGGT
                     GALRMLAELIATAHPDATVWLSDPGYLNHRPLMGAAGLKLATYPWRSARGVANVDPML
                     DALRAARAGDVVLVQGCCHNPTGLDLPPEGWAALAALCGERGLVPLVDLAYHGLGDGL
                     DADAAGLRCLVDRLDTVLIAASCSKNMSLYCERVGAALAVVPTPAAATSARSVMENIA
                     RRTYSMPPDHGAAIAAAILAAPAQWHAELDAMRQRIRAVRAALVDALRLAGAPGALLD
                     MAHHRGMFSVLPFTPPQMTALRDRYGLYGTPGGRINIAGIPACRVGQVADAIAAIALQ
                     TEGIMQAA"
     gene            complement(6572..7507)
                     /locus_tag="VKQ27_06320"
     CDS             complement(6572..7507)
                     /locus_tag="VKQ27_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY88582.1"
                     /translation="MATTLPFADPSPASDRRRAIVGWGALSATILIWASFSLSIRAIG
                     ASPLTPADVALIRYGVPAVLLLPWLPSRLRALASIPWRPAAMIAIGAGLPFFLLSAAG
                     GRLTSAAHVSALIAGTAPLAVALLGRLFCRQRIDGTLLPGLVTIVAGVALMIAGLVAP
                     GATLVLGVALLLGASLLWGGYTLALRRVTLDPLGCVMLVTYPSLLVGAPLMAGGLLDS
                     HLQQAPAAALLPFVLVQGVAVGIASTLTYAIAIRRLGALRCATAGSLAPVLAALLAVP
                     LLGETPSLVSALGIAAITSGILLSNLRSAKGMRRC"
     gene            7617..8078
                     /locus_tag="VKQ27_06325"
     CDS             7617..8078
                     /locus_tag="VKQ27_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLY88583.1"
                     /translation="MDDKDRQIIAVLQAEGRISNQELSDRVNLSPSPCLRRLRLLEEA
                     GIIRGYSAIVNEEAFGLPVTAFVRVRLQQHGTDSVAAFERAVQAMDEVMDCYVMTGSA
                     DYLLRVLADSLRGYEMFIRTKLHQAPGVTSIDTSFAYGHVKKTTVFPSAQT"
     gene            complement(8137..8253)
                     /locus_tag="VKQ27_06330"
                     /pseudo
     CDS             complement(8137..8253)
                     /locus_tag="VKQ27_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1479 a         2848 c         2710 g         1307 t
ORIGIN      
        1 ccgcgcgcct cgatccccga gctggtgcgc gccgatcccg cgctcaccgc cgcgctcgcg
       61 ctgctgcacg tcgcggcacg ggggatcggt tcccaccggc tcgtcgctgc gcccgccgtg
      121 atcgcccgaa tcgaggcacg acccgactgg atcgaggcac tggcgcggcg catcggcggc
      181 agcatcggct tgcgggccga tcccgcgctc gccatatcgg cgggccatgt cgaaccctga
      241 tccccgccgc gatccccggc gccgccgcgc agacgcctgc ccgatctgcg gcaagcccag
      301 cgccgccccg cacaccccgt tctgcgggca gggctgtcgc gaccgcgatc tgctgcaatg
      361 gctgggcgag ggctatcggg tgcaggggcg gccgatggaa ttggatggcg aagaagggct
      421 ggacagcgcc gccaacctgc ctctataggc gcgcctcgcc caacgcggca gcaagggccc
      481 gggtagctca gttggtagag cacgtgactg aaaatcacgg tgtcggcggt tcgattccgt
      541 ccccgggcac catcgccccc tccaaagaca tccacccaca tccacaaaag cccggatttc
      601 cgcggttttg aacggtattt gatccgtccg aaatcgggcc gatccgttgc catccagcgg
      661 cgctgacggt attttgacgg tatctgatca caggagtggg gccagatacc gtcatgctca
      721 ccgacgcagc catccaccgg atcaaagcgc gcgataaacc ctataaagtc agtgacatgc
      781 acgggctcta cctgctcgtc cgaccggatg gcgcacggta ttggaggatg gactaccgcc
      841 atgccgacaa acgaggtacc gtcgcgctgg gggtttatcc cgccgtttcc ctcaaggaag
      901 cccgcgaaaa gcgagagaat gcccgcaagc tcctcgataa ggggatcaat ccgtcgagct
      961 accgaaagct gacccgcgcg gtgggctcca tatcgagcgg cgtcaccttc aaggccgtcg
     1021 ctgacgaatg gctcaacaag ctcgaggcgg aaagacgatc ggccgcgact ctcgataaga
     1081 tgcgctggct gctccgattc gccgagccgc tcatcggcgg gcgccccatc ggggagatca
     1141 ccgcgcccga actcctcacg gttctgagaa ccgtcgaggt ccgcggccga tatgaaaccg
     1201 cccggcgact ccgagggacc tgcggcagca taatccgtta tgccattgcc acgggacgcg
     1261 ccgagcgcga cgtcacgacg gatcttcatg gggcgctcat cactccccgg gtgaagcatc
     1321 acgcctccat caccgagccg aacggcgtcg gcgagctgct acgcgccatt gaagactatc
     1381 ggggacagcc gacggtcgcg atcgcgttgc gcctcgcgcc ccacctcttc gtccgccccg
     1441 gcgagctccg ctctgccgaa tgggcagaat tcgatctggc cgcggcggtc tggaccattc
     1501 cgggcgacaa gatgaagatg gggcgctctc acaaagtgcc gctctcccgc caggtgcagg
     1561 cactgctcaa ggatctggcg ccgatcagtg gtcgatcgaa attcctcttc ccgggcattc
     1621 gctcgaacga tcgggcgatt tccgagaata cgatcaatgc ggcgttgcgc cggcttggat
     1681 atggcaagac cgaaatgacc ggtcacggct tccgcgccat ggcgagcagc ctgctcaacg
     1741 aaagcaggaa atggcatccc gacgcgatcg aacgccagct cgcccatgtc gaaagcaatg
     1801 atgttcgccg gacctatctg cggggcgagc actgggccga gcgggtgaag atgatgcagt
     1861 ggtggtcgga ttatctcgat cagctcagat cgcggggaaa gatcgtccgc cggaaatttg
     1921 gctagccctg atcccgggga gctgccgacc ctgcccagtc tcggcgatct gggtggctgc
     1981 gtagaccgga cgagcacggg ccccggacta tgttcatgct acatcttgcc gaggcgctgc
     2041 atgagttcgt cgctatggat cacgaagcgg ccccagggcg tataggcact gccatgcgcg
     2101 catttctctc tggaactctc ctccttgccg gtctcgtcag cggcagcagg gccctggccg
     2161 atcccgcgcc tttcgacctt gcggggccgg cgctccgcgt cactgtcacc catcaggggg
     2221 tgaccttgcc gattgccgag gtgccgcagc tggcggccgg cgatagtgtc aaggttcgcg
     2281 ccgacctgcc cgatgaccag tccgcccact atgtcctcgt cgccgccttc ctccgcggat
     2341 caaccaatcc gccgccggac aaatggttct atcaggcaga gagctggacc aagaaaggcc
     2401 gcaagggact gtcactcacc gtgcccgatg gcgcgcaaca gatcgttctg ttcctcgctc
     2461 ctgcaacagg cggcgactat ccgaccctgc gtaacaccgt gcaaggtcgc ccaggcgcct
     2521 tcgtgcgcgc ggcgcaggat ctcgctcagg cctcgctcga ccgtagccgg ctggacacct
     2581 atctcacggc cgtccgcaag accaatccgg gcgaccccaa tcggttggac cggatcacac
     2641 cgctcctggc gcgtaccctc caggtaaagg tcaacaccga ctgcctgacg aagacgccgg
     2701 agctgcaggc tgcctgcctg ctccaggacc aggaatcgct ggtgctcaac gatggccaca
     2761 gcaacgccat caccgaggcc gtggcggggc cgggcgccga tctcgcgctc cagctcagcg
     2821 ccacgccgca gggaggcctt ggctactaca gcccctacat cgctgcgatc cgcgacatca
     2881 tcggcatctt cagctcgatc cacaccgcca aataccagta tattccagca ctcgcgacgc
     2941 tcagcaatga tcgcatgggg cttgtgctca acgccccgcc gtcgttccac aatcccaagt
     3001 cggtgctggt gacggcccta ccgatcgtgg cgccggtgca tgtcccgccg ctacaggtgg
     3061 ccaatcaggc gccctcgctt tgcgcccagg cgccggaact gctgctgccg atcagcggag
     3121 cgcctctcat ctacgcgacg cgctatgcgc atgatctcag cctgcgcgtg cggctgccca
     3181 acggccggtc cattgacctt ccggcgacgc ccgacgccga gaggggcgga ctcgtcatcc
     3241 agacgcaagg caaattgcca gccgacctca aggtgccgct cgatgcgtcg ctgcatggca
     3301 tctggggctt ccagcccttc gatgggccat ctgtgcgcct gcagccggcc cagcccggcc
     3361 agtggcgact gccgccgggt gcagccacag ccccaaacgc gaccgtcaca ctgactggcg
     3421 gcacggcggc ttgcgtgtcc ggcgtctcga ttcaatccgg cggcacggcg cagccggtga
     3481 gctggacggc tgccaagagc gatgagatca gggtcaccct tccggcaatc gacgacaagt
     3541 caggcgcgac gaccctcgaa atcaccggcc cggccggtct cccgcccgac cggctgacca
     3601 tcgcgggacc gccgccaccg ggccatttcg gcaccactct gatcgcccgc tccatgcaac
     3661 gaccaccagc gacggcgccc gtcgcgatca agcttggcag cgacagcgag gttcccagcg
     3721 acggaacgct cgccttctcg ctccgcgcca acaagggagc ccgtttcact ggacgcgaga
     3781 aggtcgaggt tgcgacggca tccggcgatg caacggcgac gctcagcccg tccaatgggc
     3841 tgacgctcgc tgatccacag gttgcgatcg cgatcatcca gccggcaaag gcgctcggtg
     3901 cgtccgcctt cgggccgatt cgcgcgcgcg tgagcaggga tggcgttgag ggggattggt
     3961 tgccgcttgg cacgctggtg cgcctgccct cactgcatca gctcacatgc ccgcctgacg
     4021 cgtctatggc ctgtgagttg acgggcgatg ggctcttcct gatcagcgcg ctttcggcca
     4081 caccgggctt cgagcatccg actgcagtac ccgagggcta tcccggcaac gcgattcagg
     4141 tgcctcatcc cgtagacggg aaactatatg tgcggcttca cgatgacccg gacactgtga
     4201 gcaacatcac cggctgagcc aaccaatccg acgctcgcta tcgggcgtcc gatcgcttca
     4261 acgccccagc gtagctctgg tgcagcaagc gaaggatccg cgggtgccgc cgatttgctt
     4321 caggatgcga agccaccctg gtagcggcga aagtcggtgg cgcgcgcagg ttgcaccatg
     4381 ccggccgagg caaagccggt ctcgtccatc cgccgggaat aggtcgccga tgggacggac
     4441 gctggagcgg ttcagggtcg atttcgcggt cttccgccgg atcttcacgc aaggtgacca
     4501 gattaaggct cctgtaccag agacacctta taaaacaggc tgtggactta tccatgggcg
     4561 gaaaggtgat ccaggagacg cctgttgcac gcgcgcctgg aattgtggcc gacctctgct
     4621 gcgtggcgga cgaacctcgg ctcaataggt gaggaacacc gcgagcaggc ccaggaggat
     4681 cagcaccagc acgaccaata tcagatcggt gggtgcgaac atcggccggc gaccatcgct
     4741 gtactcggca taccgatagg tctcccaagc cgacggctcc cgtgggtcgc tcgcgccttc
     4801 atcctgcccg gtcaatcgaa gccgtgtatc gagatagcgc ctgatctcta cgagattgct
     4861 ctcgatatcc gttccgtcga taggcttgcg ttcgcgcacg gcgctcttga tccttctgcc
     4921 gctgaccagg ctactccggc cttgcccaga gtccagccag cgtggctgcg gctcgtgcga
     4981 gcgcagtgtc tcgcaggtgc ccgaatgctg cgtcccacag agcggactga cgacctgtgg
     5041 gtgaagcgga aggaacatgg atcagacatg ggcgctgatc gtagccgata ggaatgatga
     5101 tcacccatcg tcgccacgcg ctccagcatg aagtcggcaa cctgttccga cgtccatgcc
     5161 ccgctggatt ctggtcgcct cccgagcgcg tcatgtcggt gaaagcgaag cccggaacgt
     5221 ggagatgcgc cgtcacccgg ctgccggtgg cttcgagcag actgtgggcg cgccttcacg
     5281 cccgccctgc agacattata ggcggtgttg ccgtgatccc ctgctttgat gaccagcccg
     5341 accctgtccc ccacgatcat ggcgggcacg ggcgctcacg ccgcctgcat gataccctcc
     5401 gtctggaggg cgattgcggc gatggcgtcc gcgacctgtc cgacgcggca tgccgggatg
     5461 ccggcgatgt tgatgcgccc gcccggcgtg ccgtagaggc cgtatcggtc gcgcagcgcc
     5521 gtcatctgcg gcggcgtgaa cggcagcacc gagaacatcc cgcgatgatg cgccatgtcc
     5581 agcagcgcgc ccggcgcacc cgcgaggcgg agcgcgtcaa cgagggcggc gcgcaccgcg
     5641 cggatccgct ggcgcatggc atccagttcc gcatgccact gggcgggggc ggcgaggatg
     5701 gctgctgcga tggcggcgcc atgatcgggc ggcatcgaat aggtgcgccg ggcgatattc
     5761 tccatgacgc tgcgcgccga tgtggccgcc gccggcgtcg gcaccacggc gagcgccgcg
     5821 ccgacccgct cgcaatacag gctcatattc ttggagcagc tcgccgcgat cagcaccgta
     5881 tcgagccgat cgaccaggca gcgcagcccg gccgcgtcgg catcgaggcc gtcgcccaat
     5941 ccgtgatagg caaggtccac cagcggcacg aggccgcgtt ccccgcacag cgccgccagc
     6001 gccgcccacc cttccggcgg aagatcgagg ccagtgggat tgtggcagca gccctgcacg
     6061 agcacgacat cgccggcacg cgccgcccgc aacgcatcca gcattggatc gacattggcg
     6121 acccctcgcg ccgatcgcca ggggtaggtc gccaatttca ggcccgctgc gcccatcagc
     6181 ggccggtggt tgagatagcc tggatcggac agccacaccg tcgcatcggg atgcgccgtc
     6241 gcgatcagct ccgcgagcat gcgcagggcg ccagtgccgc cgaccgtctg caaggtgacg
     6301 gtccggtcgt cccgggaagc gtcgccgacc agcagcagcc gcgtcacacc ggcgttgaag
     6361 gcggcgttgc cggccagcgg gcgataggct ttcgacggcg cgaaggccgc gagttgccgt
     6421 tcgaccgtca gcacgctcgc caacaccggg gtgcgcccgg aatcgtcgcg atagatgccg
     6481 aggcccagat ccatcttgtc cggccggcta tcggcctcga aggcggcggt gatcgcccag
     6541 agcggatcgg gcggggcgga tgcgaggcgg gtcagcatcg gcgcattcct ttggcggagc
     6601 ggagattgga gagcaggatg ccgctggtga tcgcggcgat ccccagggca gacacgaggg
     6661 agggcgtctc cccgaggagc ggaacggcga gcagcgcggc gaggacgggg gcgagcgacc
     6721 ccgccgtggc gcagcgcaac gcgcccagcc ggcggatggc gatcgcatag gtgagcgtgg
     6781 atgcgatccc gacggcgacg ccctgcacga gcacgaaggg cagcagtgcc gcggccggcg
     6841 cctgctgcag gtggctgtcg agcagccctc cggccatcag cggcgccccg accagcagag
     6901 agggataggt caccagcatc acgcagccca gcggatcgag cgtcacccgc cgcagcgcga
     6961 gcgtatagcc gccccacagc aggctcgcgc cgagcagcaa cgcaacgccg agcaccagcg
     7021 tcgcgcccgg cgcgaccagc ccggcaatca tgagcgcgac gcctgccacg atcgtcacga
     7081 gccccggcag caacgtgccg tcgatccgct gccggcagaa cagccggccg agcagcgcca
     7141 ccgccagggg tgctgtgccg gcgatgagcg cgctgacatg cgcggccgag gtgagccgcc
     7201 cgcccgccgc ggacagcagg aagaagggca gccccgcgcc gatcgcgatc atggcggccg
     7261 gtcgccacgg gatggaggcg agcgcgcgca gccgcgacgg cagccacggc agcagcagca
     7321 cggccggcac gccgtagcgg atcagcgcga cgtcggcggg ggtcagcggc gaggcgccga
     7381 tcgcgcggat cgacagcgag aagctcgccc agatcaggat ggttgcagag agcgcgcccc
     7441 agccaacgat cgcccgccgg cgatcggaag caggcgacgg atcggcaaag ggcagggtgg
     7501 tggccacaga cgctcctcgt tgggagcgct tcatctattc cgcgatgccg gggcatgtcc
     7561 tttcagaatt agcctcaggg gctgacgaga ttggcagaat ctgccacgat atgtcgatgg
     7621 acgacaagga ccgccaaatc atcgccgtgc tgcaggcaga gggccggatc agcaaccagg
     7681 agctttcgga ccgcgtcaac ctgtcgccct cgccttgtct gcggcggctg cggctgctgg
     7741 aagaggccgg aatcattcgg ggatacagtg ccatcgtcaa cgaggaggcc ttcggccttc
     7801 cggtgaccgc cttcgtccgc gtacgccttc agcagcatgg cacggacagc gtcgccgcgt
     7861 tcgagcgggc ggtgcaggcg atggacgagg tgatggactg ctacgtcatg acgggcagcg
     7921 ccgattatct tctacgcgtg ctggccgata gcctgcgcgg ctacgagatg ttcatccgca
     7981 ccaagctcca tcaggcgccc ggcgtcacat cgatcgacac cagcttcgcc tacggccacg
     8041 tcaagaagac gacggtattc ccctccgcgc agacctgacg agggtggccg ttaaccgcct
     8101 taggatttgg gtacggttcg ccggtccggg ccacagatag atcggtgcaa ggcccatctt
     8161 cgccatttgg cgccggatgc gacggcgacc aacatcgtgg cccgcacgcc gcagttggcg
     8221 aagcatctcg cgtccgccat accacggcat attagcgctt tttgcccgac ggctacgcgt
     8281 agagtcagga ggtggcgcgg tgtcgtgcgg ctcgcctgtg gggctcacca cacgatccgt
     8341 ttcc
//
LOCUS       DATGEM010000197         6153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_745027, whole genome shotgun sequence.
ACCESSION   DATGEM010000197 DATGEM010000000
VERSION     DATGEM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745027"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="VKQ27_06335"
     CDS             <1..1895
                     /locus_tag="VKQ27_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY88584.1"
                     /translation="GYSHLYGPGFYVPVGGAGGVTELARIDYSARYGPHGAQREVWME
                     LAQARKARIVPRTGGRALADALVAQGIDHVFAVPGESYLDVLDGLYEVRHKLKLVTCR
                     FEAGAVNMAEAYGKLTGRPAAAMVTRGPGACHGSIGVHIAMQDSTPMLLFVGQIPLED
                     AGRDAFQEVDYRRMFSPLAKWVTQIDQARRIPEIVAHATDVAVSGRPGPVVIALSEEM
                     QKDMIEVPDLPRASVTPAFPDPSALAQLRVMLSRARKPLAVLGGSCWTAEGRAAIRDF
                     LLGNDIAVTVGFRRQSLYDGTLDNFAGDLGVGSDPGLVGKVKEADLILAIGSRLGDTV
                     SQGYTLFDMAGRVPIIQVHPDASEIGRVFRPTLGIVSDLNTFAQAVATLEPVPSEWVD
                     WTEDLRSLREAQRAVPNYQGALNLGTAMRELEQLLEPDALVTTDAGNFAGWATRFINF
                     RDGQRYIGPTNGAMGYSVPAAIGAKIAFPDRMVVSLVGDGGFLMTGQELATAFHHGVA
                     PIVLVFNNQMYGTIRMHQERAYPGRVSGTGLTNPDFAKFIEAFGGHGEVVSETAEFSP
                     AFQRAVDSGKPAVIELRMNPEQITTRSTIAELRAGRAAKVPKKPKRAAAPKHPAPSRP
                     PSKKRV"
     gene            1948..3480
                     /locus_tag="VKQ27_06340"
     CDS             1948..3480
                     /locus_tag="VKQ27_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HLY88585.1"
                     /translation="MDAAASYYAAHDYDEAERCCGELIAQDPRHFDALHLLGVVHLDR
                     GRPTDAADCLKRAVAEREHDAQVNYHLGTALLGDGKFADAEAALRKSLLVRPDDVGAL
                     NNLGNALIGQERYPEALVCFERVLRQEAAHLPARYNAARTLAALDRMDEAIAGFQSVL
                     ADAPSDTRPDRLADIYSELGQALAQARRFEEAIVVCRALAAIKPGVAAWHESLILLLR
                     GDFAEGWRKYESRWDVPEHDRPREDAQVPALTEMAGKHVLLVPEQGHGDLIQFSRYVP
                     LLAAHGAQVSVQTYAELQALILTLDGVGAVVNPDEAEPASDIVTPLLSLPLVFGTDLA
                     SVPNRVPYLRAPADRLRVWRERLGKHTRRRIGLAWWGTQHIPKRSVPFAALQPILAAG
                     DTEFHSLQKEPAGDDASLIDHHAELQDFADTAALISLLDVVITIDTAVAHLAGALGKP
                     VWIMLHHNADWRWLLERDDSPWYPTARLFRQKQAGDWAGVVAEVVRALGSVRRPRAPV
                     RT"
     gene            complement(3449..4288)
                     /locus_tag="VKQ27_06345"
     CDS             complement(3449..4288)
                     /locus_tag="VKQ27_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HLY88586.1"
                     /translation="MRQLVQPGPIHPERVESHEGRSHRLEFSLQAGLSLNDALTRPLV
                     AAGMQCAALVFHGGALGPFSYVMPGPPDGPSHVAYFSAPSSPPSETVVEVANATFGWR
                     DDAPFVHCHGAWIERGTDRRGGHMLPHETIIARETTVHAWALPDVVISADPDPETNFT
                     LFHPVSTTVMADSGDRTIVARIRPNEDITLAIEVIARRHHMHNAIVRGSLGSLIGAHF
                     TDGRKVPDHATEVLVQSGHVHDGEAVLDMLVVDMNGHAHEGRLVRGDNPVCITFELVL
                     AAA"
     gene            complement(4377..>6153)
                     /locus_tag="VKQ27_06350"
     CDS             complement(4377..>6153)
                     /locus_tag="VKQ27_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HLY88587.1"
                     /translation="MFTTRPEIAGTFGVVASTHWIASQVGMAVLERGGNAFDAAVATA
                     FTLQVAEPHLNGPGGDAPIIIHSARTQTQQVICGQGVSPQAATLEKFRELDLDLIPGT
                     GVLPAVVPGAFDAWCLLLRDWGTWELPDVLSFAIGYASNGVHIVPRISAAIEGVRMLF
                     EAEWPSSAAVFLPNGKVPAPESLFARPALAATYERICREAAGDSREARIDAARSAWYR
                     GFVAEAVDKFFRTTDVLDASGRRHRGLLSADDFAGWSATVEDPVSYDYHGHTVLKCGP
                     WSQGPVFLQQLALLQGFDIGAMDPFGAEFVHTVVECAKLAYADREAYYGDPNFVRVPL
                     STLLSDDYNTARRKLVGRDASQELRPGAVPGYIPTVDRDAAQRAYLLNRAPGIGEPTV
                     ARLGVIGADTCHLDVIDRDGNMVSATPSGGWLQSSPVIPELGFCMGTRGQMFWLKPDL
                     PNSLEPGKRPRTTLSPSFALRDGKPWMAFGTPGGEQQDQWSLTFLLRMIHHNMGLQEA
                     IDAPSFHTEHWPSSFWPRVARPGKLVLEGRFPNAVMYELKARDHKAEKGDDWSEGRIS
                     GARIENGQMRAGANPRGMQGYAVGR"
BASE COUNT         1055 a         1844 c         2087 g         1167 t
ORIGIN      
        1 gcggctattc gcatttatac ggaccgggat tctacgtacc ggttggaggt gccggtggcg
       61 tgactgaact ggcgcgaatt gactattctg cgcgctatgg cccgcatggg gcgcaacggg
      121 aggtctggat ggaactcgca caggcgcgca aggcgcgcat cgtcccacgc acgggggggc
      181 gggccttggc ggatgcgttg gtggctcaag gtattgacca tgtgtttgct gtgcctggcg
      241 aaagctatct cgacgtgctc gacggcctct atgaggtgcg tcacaagttg aagctggtga
      301 cctgccggtt cgaagccggt gcggtgaaca tggccgaggc ctacggcaag ctcaccggtc
      361 gtcccgccgc ggcgatggtg acgcgcggac ctggtgcctg tcatggttcg atcggtgtgc
      421 acatcgccat gcaggacagc acgccgatgc tgctgttcgt cggccagata ccgttggagg
      481 atgcgggccg cgatgcgttc caggaggtgg actatcgccg gatgttcagc ccgcttgcca
      541 agtgggtgac gcagatcgat caggcgcggc gcatccccga aatcgttgca catgcgacgg
      601 atgtcgccgt gtcggggcgg cctggtccgg tggtgatcgc actctcggaa gagatgcaga
      661 aggatatgat cgaggtcccc gatctgccgc gtgcttcggt cacgcccgcc tttcctgatc
      721 cgtcggcgct tgcgcagttg cgtgtgatgc tgtcgcgcgc gcgcaagcct ctggcggtgc
      781 tgggcgggtc gtgctggacc gcggaaggcc gtgctgcgat ccgtgacttc ctgcttggca
      841 atgacatcgc ggtgaccgtc gggttccgtc gccagtcgct gtacgacggc acgctggaca
      901 acttcgccgg cgatctcggc gttggttccg atccaggact cgtcggtaag gtcaaggaag
      961 ctgatctgat cctggcgatc ggctcgcggc tcggtgatac tgtgtcgcag ggttatacgc
     1021 tgttcgacat ggccggcagg gtgccgatca ttcaggtcca tcctgatgcg agcgagatcg
     1081 gccgcgtgtt ccgtcctacg cttgggattg tgtccgatct gaataccttc gcgcaggctg
     1141 tcgctacttt ggagccggta ccatccgaat gggtggactg gaccgaggat ctgcgatcgc
     1201 tgcgggaggc ccagcgtgcc gtgccgaact atcaaggtgc gttgaacctg ggcacggcga
     1261 tgcgcgaact cgagcaactg ctggaacctg atgcgctggt caccacggat gccggcaact
     1321 tcgcgggctg ggcaacgcgg ttcatcaact tccgcgatgg gcaacggtat atcggcccga
     1381 cgaatggcgc gatgggttac agcgtgccgg cggcaatcgg cgccaagatc gcttttcccg
     1441 atcgcatggt tgtcagcctg gtgggcgacg gcggtttcct gatgacgggg caggaactgg
     1501 cgaccgcgtt ccatcacggt gtcgcgccga tcgtgctggt gttcaacaac cagatgtacg
     1561 gcaccatccg gatgcatcag gagcgtgcct atccgggtcg cgtgtcaggc acgggcctca
     1621 ccaatcccga tttcgccaag ttcatcgagg cgtttggcgg tcacggcgaa gtggtcagcg
     1681 agacggcgga gttctcaccg gcgtttcagc gggccgtgga tagcggcaag cccgcggtga
     1741 tcgagctgcg catgaacccg gagcaaatca cgacgcgttc caccattgcc gagctgcggg
     1801 cagggcgtgc ggcgaaggtg ccgaaaaaac caaagcgtgc cgcggcgcca aagcatccgg
     1861 cgccgtcgcg tccgcctagc aagaagcgtg tgtagctaca ggctgagtac gcagttccgg
     1921 atcgatgcgc cactgccggc gcatggcatg gatgccgcag ccagctatta cgctgcgcat
     1981 gattacgatg aggccgaacg ctgctgcggt gagctgatcg cgcaagaccc gcgtcacttc
     2041 gatgcgctgc atctgctggg tgtcgtgcat cttgatcgcg ggcgaccgac ggatgctgct
     2101 gactgtctga agcgtgctgt ggctgagcgt gagcatgatg cgcaggtcaa ctaccatctg
     2161 ggtacggcgc tgctcggcga tgggaagttc gctgacgccg aggccgcctt gcgcaaatcg
     2221 ctgctagtgc gccccgatga tgtgggtgcg ctcaacaatc tgggtaatgc gttgattggt
     2281 caggagcgat atcccgaggc attggtatgt ttcgagcgtg tgttgcggca agaggccgcg
     2341 catttgccgg cacgctacaa cgccgcacgt acgctggccg cgctcgaccg catggatgag
     2401 gcgatcgcgg gctttcaatc cgtgttggcg gacgcgcctt ccgatacgcg accggatcgg
     2461 cttgccgaca tctattccga acttggccag gcgctggcac aggcaaggcg tttcgaggaa
     2521 gcgatcgtcg tttgccgtgc ccttgctgcc atcaagcctg gtgtggcggc gtggcatgaa
     2581 agcctgatcc tgctgttgcg aggcgacttc gccgaaggat ggcggaaata cgaaagccgc
     2641 tgggacgtgc ctgaacacga ccggccgcga gaggatgcgc aggttccggc gcttaccgag
     2701 atggccggca aacatgtgct gctggtgccg gagcaggggc atggcgacct tatccagttc
     2761 tcgcgttatg tgccgctgct ggcggcgcac ggcgcgcagg tcagtgtgca gacttatgcc
     2821 gaactgcagg cgttgatact gacgctggat ggtgtcggtg cggtggtgaa cccagatgag
     2881 gcggagccgg ccagtgatat cgtcacgccg ctcctcagcc tgcccctggt gttcggcacc
     2941 gatctggcaa gtgttccgaa cagggtgccg tatctacgcg cgccggccga ccggctgcgg
     3001 gtgtggcgcg agcggctcgg caagcacacg cgccgtcgga tcggcctggc gtggtggggt
     3061 acgcagcata tcccgaagcg gtcggtaccg tttgctgcgt tgcagccgat cctggcggct
     3121 ggcgatactg agttccattc cttgcagaag gagccggccg gcgacgatgc atcactgatt
     3181 gaccatcacg ctgagctgca ggacttcgcc gataccgcgg cgttgatatc gctattggat
     3241 gtggtgataa cgatcgatac ggcagtggca catctggctg gcgcgttggg caaaccggtg
     3301 tggatcatgc tgcatcacaa cgccgactgg cgatggctgc tggagcgtga cgacagcccc
     3361 tggtatccga cggcccggct gtttcgccag aaacaggccg gcgactgggc gggcgtggtg
     3421 gccgaggtgg tgcgcgcgct gggatcggtc aggcggccgc gagcaccagt tcgaacgtga
     3481 tgcagacggg attgtcgccg cgcaccagac gcccctcatg cgcatggcca ttcatatcga
     3541 caaccagcat gtccagcaca gcttcgccat catgcacgtg gcctgattgt accaggacct
     3601 cggtggcgtg atctggtact ttcctcccat cggtgaaatg cgcgccgatc aggctgccga
     3661 ggctgcctcg cacgatggcg ttatgcatgt ggtggcgccg tgcgatgact tcgatcgcca
     3721 gtgtgatatc ttcgttgggc cggatccttg caacgatggt gcggtcgcca ctatccgcca
     3781 tgactgttgt cgagaccgga tggaacaaag tgaagtttgt ctcgggatca ggatcggcgc
     3841 tgatgacaac gtcgggcagc gcccaggcat gtacggtcgt ttcacgtgca atgatcgtct
     3901 catgcggcaa catatggccg ccgcgtcggt ctgtgcctcg ctcgatccac gccccgtggc
     3961 aatgcacgaa cggtgcatca tcgcgccagc cgaaggtggc gttggcgact tcgaccacgg
     4021 tttcactggg gggcgaactc ggcgcactga aataggcgac gtggctgggg ccatcgggcg
     4081 gacctggcat cacgtagctg aatgggccga gcgcgccgcc gtggaacacc agcgcggcgc
     4141 attgcatgcc tgctgcgacc agtggcctgg tgagcgcatc gttcagagac aagccggcct
     4201 gtagtgaaaa ctccagtcgg tgactgcgtc cttcgtggct ctcgacacgc tcgggatgga
     4261 tcggtcccgg ttgaacaagc tgccgcatca catatcgttc cgggcgtgag cctgggcttc
     4321 ttgcgtcatg gctggaccca aagtcctcgg gcctgtcccg gggatcagat cggccatcag
     4381 cggccgaccg cgtagccttg catgccgcgc gggttggcac cggcgcgcat ctgcccgttc
     4441 tcgatccgag cgccggagat gcggccttcc gaccagtcgt cgcctttctc ggccttgtgg
     4501 tcgcgcgcct tcagttcgta catgacggca ttcgggaaac gaccctcaag caccagcttg
     4561 cctggccgcg cgacacgcgg ccagaagctg gagggccagt gctcggtatg gaacgaagga
     4621 gcgtcgatcg cttcctgaag gcccatgttg tggtggatca tgcgcagcag gaacgtcagc
     4681 gaccattgat cctgctgctc gccgcccggc gtgccgaacg ccatccaggg cttgccatcg
     4741 cgcagcgcaa agctgggtga aagcgtggtg cgggggcgct tgcctggctc cagactgttc
     4801 gggagatcgg gcttcaacca gaacatctgg ccgcgggttc ccatgcagaa gccaagctcc
     4861 ggaatcaccg gcgatgattg cagccagccg ccagatggtg tggcactgac catgttgccg
     4921 tcgcgatcga tcacatccag gtggcatgtg tcggcgccga tcacaccaag ccgcgcaacg
     4981 gtgggttcgc cgatgccagg cgcgcggttc agcaggtagg cgcgttgcgc cgcgtcgcga
     5041 tcgacggtcg gtatatagcc aggcacggcg ccgggccgca gctcctgcga tgcatcacgc
     5101 ccgaccagct tgcgacgtgc agtgttataa tcgtctgata gcagcgtgct cagcggcact
     5161 ctgacgaagt tgggatcgcc gtaataggcc tcgcggtcgg cataggcgag cttggcgcat
     5221 tcaaccacgg tgtggacgaa ttccgcgccg aacgggtcca tggcgccgat atcgaagcct
     5281 tgcagcaggg cgagttgttg caggaagacg gggccctggc tccacggacc gcacttcaac
     5341 accgtatggc cgtgataatc gtacgatacg gggtcttcca cggttgccga ccatccggcg
     5401 aagtcatcgg ccgacagcag cccgcgatgg cgccggccgg acgcatcgag cacatcggtg
     5461 gtgcggaaga acttgtccac tgcctcggcc acgaagccgc gataccaggc ggaccgcgct
     5521 gcatcgatcc gcgcctcacg cgagtcgccg gctgcctcac ggcagatgcg ttcgtaggtc
     5581 gcagcgagag cgggccgtgc gaacagactt tccggcgctg ggactttgcc gtttggcagg
     5641 aacacggctg cggatgacgg ccattccgct tcgaacagca tgcgcacgcc ctcgatcgct
     5701 gcactgatgc gcggcacgat atgcacaccg ttgctggcat agccgatggc aaaggacagc
     5761 acgtcgggca attcccacgt gccccagtcg cgcagcaaca ggcaccatgc gtcgaatgcg
     5821 cctggaacca ccgcaggcag cacgccggtt cctgggatca gatccaggtc cagctcacgg
     5881 aatttctcca gcgtcgccgc ctggggcgac acgccctggc cgcagatcac ctgctgcgtc
     5941 tgggttcgcg ccgagtggat gatgatcggc gcgtcgccgc ccgggccgtt gagatgcggc
     6001 tccgctacct gcaaggtgaa tgccgttgcg accgcggcgt cgaacgcatt gccgccgcgc
     6061 tcgagcacgg ccatgcccac ctggctggcg atccagtgcg tgctggcgac aacgccgaat
     6121 gtgccggcga tctccgggcg ggtggtaaac atg
//
LOCUS       DATGEM010000198        13082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1565843, whole genome shotgun sequence.
ACCESSION   DATGEM010000198 DATGEM010000000
VERSION     DATGEM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565843"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..580)
                     /locus_tag="VKQ27_06355"
     CDS             complement(65..580)
                     /locus_tag="VKQ27_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLY88588.1"
                     /translation="MVRATTSQQAEPAIPVPVKISVSLIVNGVEKRLMLAPWTTLLDA
                     LREHLDLTGTKKGCDHGQCGACTVLMEGRRINSCLTLAVMQDGAKITTIEGLATDGTL
                     HPLQQAFIDYDAFQCGYCTSGQICSATGLIAEGHAHTADEIRELMSGNICRCGAYPNI
                     VKAIQQVMGQP"
     gene            complement(748..2532)
                     /locus_tag="VKQ27_06360"
     CDS             complement(748..2532)
                     /locus_tag="VKQ27_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HLY88589.1"
                     /translation="MQERDLRAAIEDVRGGRMSRRWFIESMVRLGVTAPLAAQMLAAS
                     GAAAATDAGYKPTKRGGGGALKLLWWQAPTLLNPHFAVGTKDQDAARIFYEPLASWSA
                     DGDLIPILAAEIPSVANGGIAKDSKSVTWRLKQGVIWHDGQPFTADDCVFNWQYASDP
                     ATATTTAGSFQDINVVKLDDHTIRVEFKAPTPFWARAFVGTNLIIPKHLFEPYSGAKS
                     RDAPANLAPVGTGPYTFVSFKPGDLVQGKLNPGYHMPNRPYFDTIEMKGGGDAVSAAR
                     AVLQTGEFDFAWNLQVEDDTLKRLEAGAKGRIEVTPGGSIEYIQLNNTDPWTTVDGER
                     SSIKTKHPFLEDPAVRNALNLLVDRASVHKYIYGRTGVDTANFLNAPERYVSKNTSYE
                     FNVAKAIKLLDDGGWKPGTDGVREKDGVKLHAVYQTSTNAPRQKTQEIVKQACQKAGI
                     SIEIKSVMASVYFSSDVANPDTTRKFYSDIQMYTTTMTQPDPAPLMRAFLASEVASKA
                     NKWQGFNITRWRNADYDKLYDQSESELDPVKRAALFIAMNDMAIKNVVVIPVVTRPTV
                     SAAANQLHAPLSGWDSDLSALHDWYRDI"
     gene            2706..3926
                     /locus_tag="VKQ27_06365"
     CDS             2706..3926
                     /locus_tag="VKQ27_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88590.1"
                     /translation="MTAKTTKTNERQALWLVSAAHMVSHFHQLVLPPLFLLLRDRLGV
                     GFIELGLAVTAYNASTALAQAPMGYVVDRIGPRRMLIFGLCLSGAALASIAAFPVYTW
                     LIGAAVVAGVANSVYHPSDYSILGTVIEPSRVGRAFSIHTFAGFLGGAIAPFVMLVLA
                     TTVGLRPALLFAAALGPIVAAALLLSPRLDQAAMARPAQRQDEASATPVLTPVVLSLT
                     GLFALLSLSSAAISTFSAVALVALYAIEPSVANAALSAYLMAMAMGVLAGGVIADATR
                     RHAEVTAAGFATAAVLVFSIGSFNLGAFFLLPAMAGAGFLAGLIMPSRDMMVRAVAPP
                     GMAGRVFGVVTTGFNIGGAIGPMLGGWCVDHGAPRWVFYSSVCFMALTVVAVLVGDWR
                     SRRRALGMALMQAE"
     gene            3933..5237
                     /locus_tag="VKQ27_06370"
     CDS             3933..5237
                     /locus_tag="VKQ27_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY88591.1"
                     /translation="MARTRTEEEQFLIDLAQRVLPAGSFGNLPGDIVIREGKGGRVWD
                     VSGNEYVDFLLGSGPMFIGHCHPDVVAAVEAQIPRGTTFFANNENGIRLAAAIVDAVP
                     CAQKVRFVSSGTEADAYAMRVARAFRGRDKIMKFEGGYHGMSDYSLMSLAPKRPGNFP
                     QAVPDSPGIPRSVRDEMIIAPFNDPAMAASLIHEHHDELGGVIVEPFQRLLPPKPGFL
                     KALRDATAEHGIPLIFDEVVTGFRFSYGGAQEFYGVTPDLCTLGKVIGGGFPLAAIAG
                     REDIMSLFDRGKVSDDRFLTQIGTLSGNPVAAAAGLATLEVLKRPGSYDKIFATGTEL
                     MTGITRMLEKAGLPAQVLGVPPLFDIVYAVGDVSDYRAWMRADTAMQRRFNQRFRDGG
                     ILKGESKFYVSLAHEAAEVKHTLDTVQAAIDAELAMSRAAAQ"
     gene            complement(5484..7187)
                     /locus_tag="VKQ27_06375"
     CDS             complement(5484..7187)
                     /locus_tag="VKQ27_06375"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLY88592.1"
                     /translation="MQKPATLAYGLEETPPASVIWISAIQHVGVCAIFMVYPLIIADQ
                     ARLPPDQVTNLLQLGFIVLAIATLLQALPRGPVGSRFLAPSIYTGVYLAPSLLAVRIG
                     GLPLMWGMTIFAGLVEIALSRVWSRLRPFIPPESAGLVIVLVGFIIGLAAVRVMLEDS
                     AAGTLTARDGLVAGCALAVMAALNVWNNGRLKLFCILIGMVVGYLAAAGVGLLTWHDV
                     SDLVHRPLLAIPTLRWTSWAFDASLVVPFAVTGLAAAMNTTAVVTTYQRLTDADWTRP
                     DMASLGRGVLADGITGAVSGLFGAYGPSVSSANVGLVAATGVASRVIAFAIAALLALA
                     AMQPTLIGVMTIMPHPVMAAAMIFTSVFIMISGVQIISTRVLDGRRTLVIGMGILSFL
                     VVSVYPNVFSRMPEWAQPLVSTPLVLATLVALSLNLLFRIGIRRTVEMTVGVGPADPT
                     RITAFIEHQAGVWGARRDVTSRVEFAVQQAIETLLGAGVATGPVKLAISFDEFVIEVV
                     MSYQGAALDLPVHPPSHDEIVETESGHQRLAGYLIRRHADRADTREKPHLVELRMHFD
                     H"
     gene            complement(7191..8294)
                     /gene="aroC"
                     /locus_tag="VKQ27_06380"
     CDS             complement(7191..8294)
                     /gene="aroC"
                     /locus_tag="VKQ27_06380"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLY88593.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPAIGCVVDGCPPRIALSEADIQPW
                     LDRRRPGQSRFTTQRQEPDEVRILSGVFDGITTGTPIAMQIENVDQRSRDYSAIAAQF
                     RPGHADLTYELKYGVRDYRGGGRSSARETAMRVAAGGVARKVLGDGVRIRGALVQIGP
                     HRVDRAHWDWDAVDDNPFFCPDPVMATEWESYLSGVRKAGSSAGAVIEVTAEGVPPGL
                     GAPIYGKLDADIAAALMSINAVKGVEIGEGFAAAALSGEANADEMRMRDGIVEFASNH
                     AGGILGGISTGQPIVARFAVKPTSSILTPRRAVDRMGQEIEISTKGRHDPCVGIRAVP
                     VGEAMLACVLADHLLRHRAQNPDAPTVARLPRN"
     gene            complement(8312..8737)
                     /locus_tag="VKQ27_06385"
     CDS             complement(8312..8737)
                     /locus_tag="VKQ27_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 family protein"
                     /protein_id="HLY88594.1"
                     /translation="MSDLYDEDILLWSEHQAELLRRLAAGEPVNERPDWANIIEEVES
                     VGRSDLSAVQSLLLQALLHMLKAQAWPGSPCVAGWAAEARLFRAQTRNAFSASMRQRL
                     DIGRIYADALVAVPANIDGQPPQPVAPQCPMTLDGLLQV"
     gene            complement(8734..9576)
                     /locus_tag="VKQ27_06390"
     CDS             complement(8734..9576)
                     /locus_tag="VKQ27_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88595.1"
                     /translation="MPDSAPLQGTLLQGKRGLIMGVTNDRSLGWGIAAACAAQGAELA
                     FTYQVEALGRRVRPLAQSLGAGDRLFMCDVGDEASIDSAFVALREHWDGLDFLVHAIA
                     FADKTYLRGRYLDTPRTAFVQALDISCYSFVAVSQRAVPMMRPGGSLLTLTYLGSERW
                     TPHYNVMGVAKAALEASVRYMAADLGGVGIRVNAISAGPIRTLASAGIGDFHYILRWN
                     QLNSPMQRNVTIADVGGAGVYLLSDLSSGVTGEVHHVDCGYHIVGMKNPAAPDISIVE
                     GGTP"
     gene            9832..10716
                     /locus_tag="VKQ27_06395"
     CDS             9832..10716
                     /locus_tag="VKQ27_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HLY88596.1"
                     /translation="MARWLLRTWSEYGQPEPVMDSIKPADIVANMVEAGVTKAGLGPL
                     DLLVRGALSGGLLGIATSLALGAAVQTGQILVGALIFPVGFVMIVLLGLELVTGSFAL
                     LPMALFARRTSPAAMITNWSWVFLGNLIGSVAYAALFVIAVTNAGQIDPAGVAERVRQ
                     LAEVKTIGYAHFGTAGMVTVFVKAILCNWMVCFGVVMAMASTSTIGRIAGAWMPILIF
                     FAQGFEHSVVNMFIIPAGMMMGAKVSFTDWWVWNQIPVTLGNLVGGALFTAGALFVTY
                     GRAASTRTMLGAAAAEGD"
     gene            10738..12492
                     /locus_tag="VKQ27_06400"
     CDS             10738..12492
                     /locus_tag="VKQ27_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HLY88597.1"
                     /translation="MADPVFTPEQQEYLKGFMAGVEARRAVAGQPLAPVDTSAPADIH
                     SAAQDRTVAAGGKLTAEEEAKRRKHPLDRFDEVAAMAAEPRFPKGTDIFLSKFFGLFY
                     VAPAQNAFMCRLRIAGGILNAHQLRGVASIAEDLGGGYADITTRANLQIREIGPDKPP
                     DVLTRLADIGLTSRGSGADNIRNITGSPTAGIDPQELIDTRPHARALHHFILHHRELY
                     GLPRKFNIAFDGGGRVAVLEDTNDIAFTAVQVADGFGVPAGVYYRLGLGGITGHHDFA
                     RTTGVIVAPADATRVAEAVLRVFIAEGDRTDRTKARLKYVLDRLGVPRFLEAMEEFLG
                     SKLTRIDPAGILPRPAQDKHGHTGVHTQKQPGLKYVGVLVPQARMPAAMMRGLADIAE
                     RFGSGTLRLTIWQNLLISDIPDASLDDALAAIAALGLDWRPSPLRGALVACTGNVGCK
                     FSASDTKRHASELVDWLEQRITIDQPLNIHLTGCHHSCAQHYVADIGLLATKVEQDDA
                     MVEGYDLLVGGGAGERQAMGRLVRKSVPFADLPPMILSLLAAWMEQRVDDEDFQCWTA
                     RQPDEALAALAERVAVPA"
     gene            12489..>13082
                     /locus_tag="VKQ27_06405"
     CDS             12489..>13082
                     /locus_tag="VKQ27_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLY88598.1"
                     /translation="MNAIVPPIPLIPENAPFSPAQRAWLNGFLAGLYGGTQTPMSVAG
                     PAAAEPEPEDFPWHDPVLELPERLAMAEGRPLSQRLMAAMGQLDCGQCGYMCQSYAAA
                     LAEGREGSISLCVPGAKPTQLALKRLMAEAPAALPAAPLPQAPAAPAGRDVLVRVSRP
                     LTGPGSSKDVRHVVVDLLESGLSYAPGDSLGLAVPNDP"
BASE COUNT         2225 a         4446 c         4052 g         2359 t
ORIGIN      
        1 gggtctgccg ctatcagccg caccgcgtcc gcgacgttct tcgcgcggac atactgaaag
       61 ccgatcatgg ctgccccatc acctgttgga tcgctttgac aatgttggga tatgcgccgc
      121 atcggcagat attgccgctc atcaattcgc ggatctcgtc ggcggtgtgg gcgtggcctt
      181 cggcgatcag cccggtggcc gaacagatct gcccggaggt acagtagccg cactggaatg
      241 catcataatc aatgaacgcc tgctgcagcg gatgcagagt accatctgtc gccaggccct
      301 cgatcgtggt gatcttcgca ccgtcctgca tcacagccag cgtgaggcac gaattgatcc
      361 gccggccctc cataagaacg gtgcatgcac cgcactgtcc gtggtcgcag cccttcttgg
      421 tgccggtcag gtccagatgt tcccgtaacg catcgagcag tgtcgtccac ggcgccagca
      481 tcagccgttt ctcgacgcca ttcacgatca gagaaacgga aatcttcact ggcacaggga
      541 tcgcaggctc agcctgctgc gacgttgttg cgcgcaccat tcgcgtctcc gcttgtcttg
      601 aggggttcac cgcagtccca cgcgatcaag taggctacac gctagcccgg aggacacccg
      661 gcgctcttga acagatcgtg cgtctgttgc ttcacgttcg cgtggttgtg gaaaactgcc
      721 tcgttggcct gtagtcgcta cggcgcgcta gatgtcccga taccagtcat gcagagcgga
      781 caggtcacta tcccaaccgc tcagcggcgc gtgcaattga ttggcggccg cggatacagt
      841 cgggcgggtt acgaccggaa tcaccacgac attcttgatc gccatatcgt tcatggcgat
      901 gaacagtgcc gcgcgcttca ccggatccaa ctcgctctca ctttggtcgt acagcttatc
      961 gtagtccgcg ttccgccagc gggtgatgtt gaacccctgc catttgttgg ccttgctggc
     1021 gacctccgat gccaggaacg cccgcatcag cggcgcagga tcgggctgcg tcatcgtcgt
     1081 cgtgtacatc tggatgtcgc tgtagaactt cctggtggta tcggggttcg cgacatccga
     1141 cgaaaagtag actgaagcca tgacgctctt gatctcgatc gaaatccccg ctttctggca
     1201 ggcctgcttg actatctcct gtgttttctg ccgcggcgcg ttggtcgatg tctggtacac
     1261 cgcgtgcagt ttcactccgt ctttttcccg tacgccgtcc gttcccggct tccacccgcc
     1321 gtcgtccagc agcttgatcg ccttggcgac attgaactca tagctcgtgt tcttcgacac
     1381 gtatcgctcc ggcgcgttca ggaaattcgc ggtatccaca ccggtgcgac cgtagatata
     1441 tttgtgcacg gaagcccggt ccaccagcag gttcagcgca ttccgcaccg ccggatcctc
     1501 caggaacggg tgcttcgtct tgatgctgga ccgctcaccg tcgaccgtcg tccaggggtc
     1561 ggtgttgttg agctggatat attcgatgct gcctcccggc gtgacctcaa tgcgcccttt
     1621 tgcgcccgcc tccagtcgct tgagggtgtc gtcctcgacc tgaaggttcc acgcgaaatc
     1681 aaattctccg gtctgcaaca cggcacgcgc ggccgacacc gcatcgccgc cacccttcat
     1741 ctcaatcgta tcgaaatacg gccggttcgg catgtggtat cctggattga gcttcccctg
     1801 taccaggtcg cctggcttga acgatacgaa cgtgtagggc ccggtgccga cgggcgccag
     1861 gttcgccggc gcgtcgcgtg acttcgcgcc gctatagggt tcgaacaggt gcttcgggat
     1921 gatcaggttg gtgccgacga aggccctggc ccagaacggg gtcggcgcct tgaactccac
     1981 gcgaatggtg tggtcgtcga gcttgacgac attgatgtcc tggaagctgc ctgccgtcgt
     2041 cgtcgcggtg gctggatcgc tagcatactg ccagttgaac acacagtcat ccgcggtgaa
     2101 cggctggccg tcgtgccaga tcacgccctg cttgagcctc cacgtaaccg acttgctatc
     2161 ctttgctatc ccaccattcg ccacgctggg gatctccgcg gccaggatcg ggatcaggtc
     2221 gccatcagcc gaccaactcg ccagcggctc gtagaaaatg cgcgcggcat cctggtcctt
     2281 ggtgcccaca gcgaaatgcg gattgagcag cgtcggtgcc tgccaccaca gcagcttcaa
     2341 cgcaccacca ccgccgcgct tcgtcggctt gtagccggcg tctgtcgccg cggccgctcc
     2401 ggatgcggcc agcatctgcg ctgccaacgg cgcggtcacc ccaagcctga ccatcgactc
     2461 gatgaaccat cgccgagaca tcctgccgcc gcgaacgtcc tcgatcgccg cacgcagatc
     2521 ccgttcctgc attgtttgtc cctcagacgg ctcggtatcg catgaggcac cggtttcgag
     2581 tccagggcaa acgatccagc ccacggcata tcgctgctga cataaccccg tcagcaggat
     2641 ggcgatagga ttggccgcat ggggccctgg tttcgaccac agggcaaatg atctaagaag
     2701 gcatcatgac ggccaagaca accaagacca atgagcgcca ggccctctgg ctggtttccg
     2761 ccgcgcacat ggtgagccac ttccaccagc tcgtccttcc gccgctgttc ctcctgctgc
     2821 gggaccgcct cggtgtcggc ttcatcgaac tcggtctggc ggtcacggcc tacaacgcct
     2881 cgactgcgct cgcgcaggcc cccatgggct atgtggtcga tcgcatcggc ccgcgccgca
     2941 tgctgatctt cggtctctgc ctgtcgggcg ccgctctcgc ctcgatcgct gccttccccg
     3001 tttacacctg gctcatcggc gccgcggtcg tcgcaggcgt cgcgaactcg gtctaccatc
     3061 cgtccgatta ctcgatactc ggcaccgtga tcgaaccgtc acgcgtcggc cgcgccttct
     3121 cgatccatac ctttgccggc ttcctgggcg gcgcgatcgc gccctttgtc atgctggtgc
     3181 tggccaccac cgtcgggctg cgccccgccc tgttattcgc tgccgcgctt ggccccatcg
     3241 tcgcggccgc gctgctgctg tcgccgcgtc tcgatcaagc cgcgatggcg cgcccagccc
     3301 agcgccagga cgaagcctcc gccacgcctg tgctcacccc cgtggtgctc agccttactg
     3361 gcctgttcgc actgctcagc ctgtcatctg cggcaatctc caccttctcc gccgttgcgc
     3421 tggtcgcgct ctatgcgatc gagccctccg tcgcgaacgc cgcgctctcg gcctacctga
     3481 tggcgatggc catgggcgtg ctcgccggcg gcgtcattgc cgatgcgacc cgccgccacg
     3541 ccgaggtcac cgctgccggt ttcgccaccg ctgcggtgct ggtattcagc atcggttcat
     3601 tcaacctcgg cgcgttcttc ctgctcccag ctatggctgg ggccggtttc ctcgccggcc
     3661 ttatcatgcc gtcacgcgac atgatggtgc gcgccgtcgc gccgcctgga atggccggcc
     3721 gggtgttcgg cgtcgtcacc actggcttca acatcggcgg cgccattggt cctatgctcg
     3781 gcggctggtg cgtggatcat ggcgcgccgc gctgggtatt ttacagctcg gtgtgcttca
     3841 tggcactgac agtggtggcg gtgctggtgg gcgactggcg atcgcgccgc cgcgcactgg
     3901 gtatggcact gatgcaagcg gagtaatcac gaatggcacg cacgcggacc gaggaagagc
     3961 aattcctgat cgacctggcg cagcgcgtgt tgcctgccgg cagcttcggc aacctgcccg
     4021 gcgacatcgt gatccgcgag ggcaagggcg ggcgcgtctg ggacgtgagc ggcaacgagt
     4081 acgtggactt cctgttgggc tccgggccga tgttcatcgg ccactgccat cccgacgtgg
     4141 tggcggctgt ggaagcgcag atcccgcgcg gcactacctt tttcgccaac aacgagaacg
     4201 gcatcaggct cgccgccgcc atcgtcgatg cggtgccctg cgcgcagaag gtccgcttcg
     4261 tgtccagcgg cacagaggcc gacgcctacg ccatgcgcgt cgcccgcgcg ttccgcggcc
     4321 gcgacaagat catgaagttc gaaggcggct atcacggcat gagcgactac agcctgatga
     4381 gcctcgcccc gaagcgtccc ggcaatttcc cgcaggcggt gcccgactcc cccggcatcc
     4441 cgcgcagcgt gcgcgacgag atgatcatcg cgccgttcaa cgacccggcg atggcggcca
     4501 gcctgatcca cgagcaccac gacgaacttg gcggggtcat tgtcgagccg ttccagcgtc
     4561 tgttgccgcc gaagcccgga ttcctgaagg cgctgcgcga tgccaccgcc gaacacggca
     4621 tcccgctgat cttcgatgag gtcgtcaccg gcttccgctt ctcctacggc ggcgcgcagg
     4681 agttctatgg cgtgacgccg gacctctgca cgctgggcaa ggtcatcggc ggcggcttcc
     4741 cgttggccgc gatcgccggc cgcgaggaca tcatgtcgct gttcgaccgc ggcaaggtga
     4801 gcgatgaccg cttcctgacg cagatcggca cgctgtcggg caatcctgtc gcggccgcgg
     4861 ctggcctggc gacgctggag gtgctgaagc gccccggcag ctacgacaag atctttgcga
     4921 ccggcaccga gcttatgact ggcatcaccc ggatgctgga gaaagccggt ctgcccgcgc
     4981 aagtactcgg cgttccgccg ctgttcgata tcgtctatgc cgttggcgat gtgtccgact
     5041 atcgcgcctg gatgcgtgcc gataccgcga tgcagcgccg cttcaaccag cgtttccgcg
     5101 atggcggcat cctgaagggc gagagcaaat tctatgtctc gctcgcccac gaagctgccg
     5161 aggtgaagca cacgctggac acggtgcagg cggcgatcga tgcggaattg gccatgagcc
     5221 gcgcggcggc ccaataggac aaaatttgcg aaccgaagcc tgaggctgga caggcaaccg
     5281 cgccgagagt ccgagcgaca cggcgcggcc gtcccgtcaa tacgagacgg acggctatcc
     5341 cagagccagc tgagactgga taaacatgag ccgggacgca ctccgccagg tcctcggctc
     5401 tttcatcgtc aacggaaccg gctgcgcgtc cgcagcctac ctatgtcgga agccgcgagc
     5461 gatcaggccc gcaagcatcc tactcagtga tcgaaatgca tccgcagttc gaccagatgc
     5521 ggcttctcac gcgtatccgc ccgatcggcg tggcgtctga tcaggtaacc tgccagccgc
     5581 tgatgtccgc tctccgtctc gacgatctcg tcatggctcg gcgggtgcac cggcaagtcg
     5641 agcgctgcgc cctggtagct catgaccacc tcgatcacga attcatcgaa gctgatggca
     5701 agcttcaccg ggccggtggc gacgccggcc cccaacaggg tctcgatcgc ctgctgcacc
     5761 gcgaattcga cccggctcgt gacatcgcgc cgcgcccccc acacaccggc ttggtgctcg
     5821 atgaacgccg tgatgcgcgt ggggtcagcc ggacccacgc cgaccgtcat ctccaccgtc
     5881 cgccggatgc cgatacgaaa cagcaggttg agcgacagcg ccaccagtgt cgccagcacc
     5941 agcggcgtgc tcaccagcgg ctgcgcccac tccggcatgc ggctgaacac attcggatag
     6001 acggagacca ccaggaacga caggatcccc atgccgatga caagggtgcg gcgtccatcc
     6061 agcacgcggg tcgagatgat ctgcacaccg ctgatcatga tgaacaccga ggtgaagatc
     6121 atcgccgccg ccatcaccgg gtgaggcatg atggtcatca ccccgatcag cgtcggctgc
     6181 atcgccgcga gggcaagcag cgcggcaatc gcgaacgcga tcacgcggct tgccacaccg
     6241 gtcgccgcca ccagtccgac attggcgctg gatacacttg gtccgtatgc accgaacaag
     6301 ccggacactg ctccggtgat gccgtcagcg agtacgcccc ggccgagcga tgccatgtcc
     6361 ggtcgcgtcc agtcggcatc ggtgagccgc tgatacgtcg tcaccacggc cgttgtgttc
     6421 atcgcggcgg caagtccggt gacggcaaat ggcaccacca gcgatgcatc gaacgcccac
     6481 gatgtccatc gcagcgttgg aatggccagc aacggacggt gcaccagatc gctgacatca
     6541 tgccaggtca gcaggccgac gcccgcagcg gccagatagc cgaccaccat gccgatgagg
     6601 atgcagaaca gcttgaggcg cccgttgttc cagacgttca gcgctgccat cactgccagg
     6661 gcgcaaccgg cgacgagacc gtcccttgcc gtcagcgtgc cggcggcact gtcctccagc
     6721 atcacgcgca ccgcggcgag cccgatgatg aagccgacga gcacgatcac gaggccggcc
     6781 gattcgggag ggatgaacgg gcgcagccgc gaccagacgc gcgacagcgc gatctcaacg
     6841 aggcccgcga atatggtcat gccccacatc agcggcagcc caccgatccg cactgccagc
     6901 agcgatgggg cgagatatac accggtatag atagacggcg cgaggaagcg gctgccgacc
     6961 ggcccccttg gcagcgcctg cagcagtgtg gcgatcgcca ggacgataaa gccaagttgc
     7021 agcaggttgg tcacttggtc cggcggcagt cttgcctgat ccgcgatgat cagcggatag
     7081 accatgaaga tcgcgcagac gccgacgtgc tggatcgcgc tgatccagat cacgcttgcg
     7141 ggcggcgtct cctccagccc gtaagcgagc gtggctggct tctgcaaagc tcagtttcgc
     7201 ggcagccggg cgaccgtcgg tgcgtccgga ttctgtgccc gatgccgcag caggtgatcc
     7261 gccagcacgc aggcaagcat tgcctcgccg accggcaccg cgcgaatgcc gacgcacgga
     7321 tcgtgccgcc ccttggtgct gatctcgatc tcctggccca tgcgatcgac cgcgcggcga
     7381 ggcgtgagaa tggaactcgt cggtttcact gcgaatcgtg ccacgatcgg ctggccggtc
     7441 gagatcccgc caaggatgcc gcccgcgtgg ttggacgcga actccacgat gccatcgcgc
     7501 atgcgcatct catcggcgtt cgcctcaccc gacagcgcgg cggctgcgaa gccctcgcct
     7561 atctccacgc ctttgaccgc attgatcgac atcagcgccg cggcgatatc cgcatccagc
     7621 ttgccataga tcggcgcccc tagccccgga ggcacgcctt ccgcagtgac ctcgatcaca
     7681 gcgccggccg atgagcccgc tttgcgcaca ccggacagat agctttccca ctctgtcgcc
     7741 atgaccgggt ccggacagaa gaacggattg tcgtccaccg catcccagtc ccaatgcgcg
     7801 cgatccacgc gatgcggccc gatctgcacc agagcgccgc gaatgcgcac accatctccc
     7861 agcaccttgc gcgccacgcc acccgcggcc acgcgcatcg ctgtctcgcg cgccgaggac
     7921 cggccgccgc cgcgatagtc gcgcacgccg tatttcagct cgtacgtcag gtcggcgtga
     7981 cctggccgga actgggccgc gatcgccgag tagtcgcgtg accgctgatc cacattctcg
     8041 atctgcatcg cgatcggcgt gccggtggtg atgccgtcga acacgccgga caggatgcga
     8101 acctcatccg gctcctggcg ctgcgtcgtg aaccgcgatt ggcctggccg gcggcgatcc
     8161 agccatggct ggatatccgc ctccgacagc gcaatgcgcg gcggacaccc atcgaccacg
     8221 cagccgatcg ccggcccatg gctctcgccc catgtcgtca cgcggaacaa atggccgaag
     8281 gtgttgtgcg acatgcggtc agctctgcac gctagacctg gagcaacccg tccagcgtca
     8341 ttggacactg cggcgcgacc ggctgtggtg gctgcccatc gatattggct ggcacggcaa
     8401 caagtgcgtc tgcgtagatc cggccaatat caagccgctg ccgcatcgac gcgctgaatg
     8461 cattgcgcgt ctgggcacgg aacagccgtg cttcggccgc ccagccggcc acgcaaggcg
     8521 agcctggcca agcctgtgcc ttcagcatgt gcaacaacgc ctgcaacaac aacgattgca
     8581 ccgcgctaag gtcactgcgt cccacgctct cgacctcctc gatgatgttg gcccagtcgg
     8641 gccgctcatt caccggttca cctgcggcga ggcggcgcag cagttccgcc tggtgctcgc
     8701 tccatagcag gatgtcttcg tcgtaaaggt cgctcatggc gtgccacctt caacgatgct
     8761 gatgtccggc gccgccgggt tcttcatgcc aacgatatgg tagccgcaat caacgtggtg
     8821 cacctcgcct gtgacaccgg atgacaggtc cgatagcagg tacacgccgg ccccccccac
     8881 atcggcgatc gtgacattcc gctgcatcgg cgagttcaac tggttccagc ggaggatgta
     8941 atggaaatcg ccaattcccg ctgacgcgag cgtccggatc ggtccagcgc tgatcgcgtt
     9001 cacccggatg cctacgccgc ccagatcggc cgccatgtag cgtacggacg cttccagcgc
     9061 cgccttcgcg acccccatca cgttgtagtg cggtgtccat cgctctgacc ccaggtaggt
     9121 cagcgtcagc aaactcccgc ccggccgcat catcggcact gcgcgctgcg acaccgccac
     9181 gaacgagtag caggaaatat ccagcgcctg gacgaacgcg gttcgaggcg tatccagata
     9241 cctcccgcgc aaataggtct tgtcggcgaa ggcgatcgca tggaccagga aatccagccc
     9301 gtcccagtgc tcgcgcagtg ccacgaaagc cgagtcgatg ctggcctcat cgcccacatc
     9361 gcacatgaac agccgatcgc ccgcgccaag cgattgcgcc aaaggccgca cgcgccggcc
     9421 cagcgcctcg acctggtagg tgaacgccag ctctgctccc tgggccgcgc aggccgcggc
     9481 aatgccccag ccgagcgatc ggtcgttcgt cacccccatg atcaggccac gcttgccttg
     9541 caaaagagtg ccttggaggg gggcgctgtc gggcatggtc gatccttagg ggccggtccg
     9601 ccgtggtcgc ataccgtcac gatactgggc aagtcctcga aggggcgcac cagaactgcc
     9661 ggaacagcgt ctggtccatc caggaaggcg ctgtcggtgc ccggtgccct gattaacaca
     9721 taggcagaac gccctcatct tcatcgtacc tagggcgccg tgacctgaaa gcgcattaaa
     9781 ccgcgccaag tccgcaccgc agcatacctg ggccgccagg aaaaagggtg aatggcacgg
     9841 tggttgctgc gcacctggtc cgagtatggt cagccggagc ccgtcatgga ctcgatcaag
     9901 cctgcagata tcgtcgcaaa catggtcgaa gccggcgtca ccaaggcggg gcttggtccg
     9961 ctggaccttc tggtgcgggg tgccttgtcc ggcgggttgc tggggatcgc gaccagcctt
    10021 gctctggggg cggcggtgca gacgggccag atcctggtcg gcgcgctgat cttccccgtt
    10081 ggcttcgtga tgatcgtgct gctcggcctg gaactggtca ccggcagttt tgcgctgctg
    10141 cccatggcgt tgtttgcgcg tcgcacgagc cccgctgcga tgatcaccaa ctggtcctgg
    10201 gtgttccttg gcaacctcat cggcagtgtc gcttacgccg cgctgttcgt aatcgcagtg
    10261 accaatgccg gccagatcga tcccgccggt gtcgcggagc gtgtccggca gctcgccgaa
    10321 gtaaaaacca tcggctatgc gcacttcggc accgccggca tggtcacggt cttcgtcaag
    10381 gcgatcctgt gcaactggat ggtctgcttc ggtgtggtga tggcgatggc atcgacatca
    10441 accatcggcc gcatcgcagg cgcctggatg ccgatactga tcttcttcgc gcagggcttc
    10501 gagcactcgg tggtcaacat gttcatcatc ccggccggga tgatgatggg cgccaaggtc
    10561 agcttcaccg actggtgggt gtggaaccag atcccggtca cgctcggcaa cctggtgggc
    10621 ggagctctgt tcaccgccgg tgcgctcttc gtcacctacg gccgcgccgc cagcacgcgc
    10681 accatgttgg gtgcggcggc cgcggaaggc gactgacgcc gcatcgccgt cgccgccatg
    10741 gccgatcccg tcttcacgcc cgaacagcag gaatacctga agggcttcat ggctggcgtc
    10801 gaggcacggc gtgcggtcgc gggccagccg ctggcgcccg tagatacgtc ggcgccggcc
    10861 gacatccaca gtgctgcgca ggaccggacc gtcgccgcgg gtggcaagct gaccgcggaa
    10921 gaggaagcaa agcgccggaa gcacccgctc gatcgcttcg acgaagtggc tgcgatggcc
    10981 gcggaacccc gcttcccgaa gggcaccgac atcttcctgt cgaagttctt cggcctgttc
    11041 tatgtcgccc cggcgcagaa cgccttcatg tgccgcctgc gcatcgccgg cggcatcctg
    11101 aacgcgcacc aactgcgtgg cgtcgcgtca attgccgagg acctcggcgg cggctacgcg
    11161 gacataacca cgcgcgccaa cctgcagatc cgcgagatcg gtccggacaa gccgcccgat
    11221 gtgctgacgc ggctggcaga tatcggcctg acgtcacgcg gctccggtgc cgacaacatc
    11281 cgcaacatca ccggcagtcc gaccgcgggc atcgatccac aggagctgat cgacacgcgt
    11341 cctcacgccc gcgcgctgca tcacttcatt ctgcatcatc gcgaactcta cggcctgccg
    11401 cgcaagttca atatcgcctt cgatggcggc ggccgcgtcg ccgtgctcga ggacaccaac
    11461 gacatcgcat tcaccgcggt ccaggtggct gacggtttcg gcgtgccggc cggcgtgtac
    11521 taccggctcg gattgggcgg tatcaccggc catcacgact tcgcccgcac caccggcgtc
    11581 atcgtcgcgc ccgccgatgc cacgcgcgtc gccgaagccg tcctgcgtgt cttcatcgcc
    11641 gagggtgacc gcaccgatcg caccaaggca cggctgaaat acgtgctcga tcgtttgggc
    11701 gtgccgcgat tcctcgaagc gatggaagag ttcctcggca gcaagctcac gcgtatcgat
    11761 cccgccggga ttctgccgcg tcccgcgcag gataagcatg gccatactgg tgtgcatacg
    11821 cagaagcagc ctggcctcaa gtatgtcggc gtcctggtcc cgcaggcccg catgccggcg
    11881 gcaatgatgc gcggactggc agatatcgcc gagcggttcg gctccggcac attgcgcctg
    11941 accatctggc agaacctgct gatcagtgac attcccgacg ccagcctcga cgatgcgctg
    12001 gccgccatcg cggcgctcgg tctggactgg cgcccgtcac cgctccgtgg cgcactggtg
    12061 gcgtgcaccg gcaacgtcgg ctgcaagttc tccgccagcg acaccaagcg gcatgccagc
    12121 gaacttgtcg actggctgga acagcgcatc acgatcgatc agccgctcaa catccatctt
    12181 accggctgcc atcattcctg cgcacaacac tacgttgccg atatcggcct gctcgccacc
    12241 aaggtcgagc aggatgacgc gatggtcgaa ggctacgacc tgctcgtcgg gggaggcgcc
    12301 ggcgagcgac aggcaatggg ccggctggtg cgcaagagcg tgccgttcgc cgatctgccg
    12361 ccgatgatcc tgtcgctgct cgcggcctgg atggagcagc gggttgatga cgaggacttc
    12421 cagtgttgga cggcgcgcca gcctgacgag gccctggcgg cgctggcgga acgggtagcg
    12481 gtgccggcgt gaacgccatc gttccgccga tcccgctgat tcccgagaac gcaccgttct
    12541 cgccggcgca gcgcgcctgg ctgaacggat ttctggcggg cctgtacggc ggaacgcaga
    12601 ccccgatgtc ggtggcaggg cctgcggcag ccgaaccgga gccggaagac tttccctggc
    12661 atgacccggt gctcgaactg ccggagcggc tcgccatggc cgagggccgt ccattgtcgc
    12721 aacgcctcat ggcggcgatg ggccagctcg attgcggcca gtgcggctac atgtgccaga
    12781 gctacgcggc ggcgctggca gaggggcgcg agggttccat cagtctctgc gttcccggcg
    12841 cgaagcccac ccagcttgcg ttgaagcgcc tgatggccga ggcacccgcc gcgttgccgg
    12901 cagcgccgct gcctcaggcg ccggcggcgc cggcggggcg ggatgtgctt gtgcgtgtat
    12961 cgcggccact gaccgggcca ggttccagca aggacgtgcg gcacgtcgtg gtggatctgc
    13021 tggagtcggg attgagctac gcgcccggcg actcgctcgg tcttgccgtg ccgaatgatc
    13081 cg
//
LOCUS       DATGEM010000199         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_174159, whole genome shotgun sequence.
ACCESSION   DATGEM010000199 DATGEM010000000
VERSION     DATGEM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174159"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VKQ27_06410"
     CDS             complement(<1..670)
                     /locus_tag="VKQ27_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLY88599.1"
                     /translation="MVFRWFESLIDAFKEPVDGMPPQSVWRFYVFYLRQVWPVFAAAI
                     VVGFGVAIVEVSLFGFVGSIVDMAKGAPAADFFKLHGRELLWMGLVALIARPVLIGLH
                     DLLVNQAIVPNLTNRIRWQNHRYVIRQSLGFFQNDYAGRIANRIMQTSGALRESAVQI
                     VDAIWYVAIYTGSAIVMFAKADYWLALPLVLWLGAYVATLAYFVPRTKERSWRQSEAR
                     SRLMG"
     gene            891..1523
                     /locus_tag="VKQ27_06415"
     CDS             891..1523
                     /locus_tag="VKQ27_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLY88600.1"
                     /translation="MRFHHCFDRHHHSHHDGREAWVREAMGIGRGRGFGRGFGDDEGH
                     GGWGGMGGRFRGGRVFGHGDLKLILLSLIAEQPRHGYELIRTIEEMFDGAYAPSPGAV
                     YPTLTLLEELGHASVASDDGKKLYTITAEGKAFLAENKEAVDAVMSRMEQTAKMFARA
                     AAPMALREAMHNLKRALFMHGGPWNAPEVKRIRDIIQKAAEEIAKGSKDA"
     gene            complement(1688..>2333)
                     /gene="nfi"
                     /locus_tag="VKQ27_06420"
     CDS             complement(1688..>2333)
                     /gene="nfi"
                     /locus_tag="VKQ27_06420"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HLY88601.1"
                     /translation="ARALQVELAAQVRLVDDHPPLRRIAGVDVGFEDDGVVTRAAAVL
                     LDAQTLAPLAEVVARQPTRMPYIPGLLSFRELPAVLQALEQLPDTPDMVFVDGHGVAH
                     PRGLGIAAHLGVTTGLPAIGVAKSILVGTHDELGQHRGDRTVLRYKGHVIGCVLRSKD
                     KVRPLIISPGHRVSMDAAPELVLAYCTKYRLPEPTRLADRLASRRDRARKASGL"
BASE COUNT          458 a          767 c          738 g          370 t
ORIGIN      
        1 ggcccatcaa ccgcgagcgc gcctcggact ggcgccacga acgttccttg gtgcgcggca
       61 caaagtaggc cagcgtcgcc acgtacgcgc ccagccacaa caccaacggc agggccagcc
      121 agtagtcggc cttggcgaac atcacgatgg cgctgccggt gtagatcgcc acataccaga
      181 tggcgtccac gatctgcacc gccgattcgc gcagggcgcc ggaggtctgc atgatgcggt
      241 tggcgatgcg gccggcgtaa tcgttctgga agaaacccag gctctggcgg atcacatagc
      301 gatgattctg ccagcggatg cggttggtga ggttgggcac gatggcctgg ttcaccagca
      361 ggtcgtgcag gccgatcagc actgggcgcg cgatcagcgc cacgaggccc atccatagca
      421 gttcgcgccc gtgcagcttg aagaaatccg ccgcgggcgc gcccttggcc atgtccacga
      481 tgctgccgac gaagccgaac agcgacacct ccacgatggc cacgccgaag cccaccacga
      541 tggcggcagc gaacaccggc catacctggc gcagatagaa cacgtagaag cgccataccg
      601 attgcggcgg catgccgtcg acgggttcct tgaacgcgtc gatcagcgat tcgaaccagc
      661 ggaacaccat gggtcgtcct tgggggaaac aacggagcgg gataactact tcgtgaaggc
      721 gcacaagaca ggtttcaagc gggattgcgc cctcccggga aacatcacca ccgggagccc
      781 cctcaccgtc atcccctcga aagcggggat gacggtgagg gtacctgttg cattccgcga
      841 atcttacgat atatcttaag actgttactt acgatatatc ggagttagtc atgcgattcc
      901 atcactgctt tgatcgccac catcattcac atcacgacgg ccgcgaggcc tgggttcggg
      961 aggccatggg catcggccgg gggcgtggct tcggccgcgg ctttggcgac gacgagggcc
     1021 acggcggctg gggtggcatg ggtgggcgct tccgcggtgg ccgggtattc ggtcacggcg
     1081 acctgaaact gatcctgctg tccctgatcg ccgaacaacc acgccacggc tatgagctga
     1141 tccgcaccat tgaagagatg ttcgacggtg cctatgcccc cagccccggc gcggtctacc
     1201 ccaccctcac cctgctggag gagctgggcc acgccagcgt ggccagcgac gacggcaaga
     1261 agctgtacac catcaccgca gagggcaagg catttcttgc cgagaacaag gaggcggtcg
     1321 acgcggtgat gtcgcgcatg gagcaaaccg cgaagatgtt cgcccgcgcc gccgcgccga
     1381 tggcgctgcg cgaagcgatg cataacctca agcgcgcgct gttcatgcac ggcggcccgt
     1441 ggaacgcgcc cgaggtgaag cgtattcgcg acatcatcca gaaagcggcg gaagagatcg
     1501 ccaagggcag caaggatgct tgaacgcgat ccgacggcgg ttgccgcggt tgcgcaatcg
     1561 gcggtggcct tcggttatcg gctgatgccg atggacgatg gcttgccgat ggaccgatag
     1621 atacgcttca tggtgccgtc attccggcgc aggccggaat gacgcacgag cctgcccgcc
     1681 gccggcatca aagaccgctg gccttgcggg cccgatccct gcgagacgct agccgatccg
     1741 ccaaccgcgt cggctccggc aggcggtact tggtgcaata cgccaacacc agctcgggtg
     1801 cggcatccat gctgacccga tggccgggcg aaatgatcaa cggccgcacc ttgtccttgc
     1861 tacgcaagac gcagccgatg acatgtccct tgtagcgcag cactgtgcga tcgccacgat
     1921 gctggcccaa ctcatcgtgc gtccctacca ggatcgactt ggccacgccg atggctggca
     1981 atccggtcgt cacgcccaga tgagcggcaa tgcctagccc acgcggatgc gctacgccat
     2041 ggccatcgac gaacaccatg tccggcgtat ccggcaactg ttcgagcgcc tgcagcacgg
     2101 ctggcagctc acggaacgaa agcagtccgg gaatgtacgg catacgcgtg ggctgccgcg
     2161 ccacgacctc cgccagcggc gccaaggtct gcgcatcgag caataccgct gccgcgcgcg
     2221 tcaccacgcc gtcatcctcg aagcccacgt cgacaccggc aatgcgccgc agtggcgggt
     2281 gatcgtccac cagcctcacc tgcgcggcca gctccacttg cagcgcgcgg gcc
//
LOCUS       DATGEM010000200         7578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1429917, whole genome shotgun sequence.
ACCESSION   DATGEM010000200 DATGEM010000000
VERSION     DATGEM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429917"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VKQ27_06425"
     CDS             complement(<1..1390)
                     /locus_tag="VKQ27_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLY88602.1"
                     /translation="MLIGFTTVAAPLLFAIVNAAVQMNRLSNRSQQLVIHGVQGTRNN
                     QSMFEEIGALERSARLYQIIGNTELLEGYTRNQARLAATIGELLTLPLDEQSHVDARG
                     LLAEADRLSRELKAAPPNSPRMAETINAFPHMSEMASTLSNRINTDINDELTSLQLAA
                     QRAQQKLFWWTLLLVPVTVAAVGFFTYLFGRPIRAIDRAISELGRGTFSRPIAISGPT
                     DLERLAAQLEWLRGRLLELAQEKNRFLRHMSHELKTPLANIREGTELLMDGAVGELQS
                     AQREVMAILRENGMKLQRLIENLLSFSAWQAKSVGLEITEFKLRPLVKSVLENQQLTL
                     VAQRVRLDVQVEDLTPCADRGKVRLILDNLLSNAIKFTPRGGTISIHARGEREQLILD
                     VMDSGPGIPAEDRNRIFEAFYQGKTPQGGHVKGTGIGLSVVTEFVNAHGGGIEIFEAK
                     TGGAHFRVRLPLR"
     gene            1451..2446
                     /locus_tag="VKQ27_06430"
     CDS             1451..2446
                     /locus_tag="VKQ27_06430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HLY88603.1"
                     /translation="MTAEAPYSDLSPEIVLDALEAAGHRCDGRVLALNSYENRVYQIG
                     TEDAEPVVAKFYRPGRWTDAAILEEHAFSLELAAQEIPVVAPLARPAEAGGQESLFVH
                     RGFRYAIFPRRGGRWPELATATDREWVGRFLGRIHGVGRADRFVHRGRLSVAELGRNA
                     RDFLLEGDWMPDYLATKYEDLTDSLLEEVEAQADGWGGARLGRILGDCHRGNILWTDL
                     GPHFVDLDDCLTGPAIQDVWMLLAGSQQEMRTDLTDLLRGYEQFLPFDRGELALIEPL
                     RALRMIHYSAWLARRWHDPAFPRAFPWFAEPRYWEEHYRALEEQLGAVLGPRLEL"
     gene            2586..3128
                     /locus_tag="VKQ27_06435"
     CDS             2586..3128
                     /locus_tag="VKQ27_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88604.1"
                     /translation="MTRGARLSLSREPRWALLAAVLLAACHGEPKQAPEAAPATAASA
                     PAVAKRGPTPEELTAGMVEAVTIGKSTAPVTIKFDLPQRPSVGKRMEIVIAVLPQIVA
                     SSAMVQVAGSEGLQLAPNFAPIEIASLDPTQVYRLSVPVTPTADGVQLLGLNVSLKVD
                     DSAESRTFSVPIIVSPGGSD"
     gene            3221..5050
                     /gene="typA"
                     /locus_tag="VKQ27_06440"
     CDS             3221..5050
                     /gene="typA"
                     /locus_tag="VKQ27_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HLY88605.1"
                     /translation="MTQKLRNIAIIAHVDHGKTTLVDQLLRQSGTLDARKDLQERVMD
                     SNALERERGITILAKNTAITWGDFRINIVDTPGHADFGGEVERVLAMVDCVLLLVDAV
                     DGPMPQTRFVTQKAFKHGLRPIVVINKIDRDEARPGWVLDQTFDLFDRLGATDEQLDF
                     PVVYASALRGFAGLDSTVRDGDMQPLFKTIVDHCPPPDVDSDGPLQLQVTLLDYSSYV
                     GAIGIGRIKRGTLRKGMAVTVVNREGKQRSERITQVLGFHGLTRIEVESAFAGDIVAF
                     AGIAEPKVSDTLCDPLNVEALPNLTVDEPTISMTFEVNTSPFLGRDGKFVTSRQIRER
                     LDRESIHNVALRVEPTSDPDKFVVFGRGELHLGVLLENMRREGYEMAVSRPRVVIKHV
                     DGVAHEPYEQVTVDADSDAQGSIMSALGSRGAELKDMQADGRGRVRLDYMVPSRGLIG
                     FQTEFRTMTRGTGLLHHVFDHYGPVVQRELGQRPNGVLIANGQGVALGYSLFSLQERG
                     RLCIGPGEEVYEGMIIGLNNRDDDLVVNPLKGKKLTNMRAAGKDENVVLTPPIRFSLE
                     QAMEFIDDDELIEVTPNSIRLRKRNLRGSDRKRSGRETVDQAS"
     gene            5181..>7578
                     /locus_tag="VKQ27_06445"
     CDS             5181..>7578
                     /locus_tag="VKQ27_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY88606.1"
                     /translation="MARDIATALRSAVAETPDSYNRVLDQQIEAARGRGGELDLDSLF
                     KQVSAHYDRLDAERRGVVLSMQLMSDEAQALTREIREETASHLQAILDNVKDAIVTVD
                     DGGHIETFNPTGERLFGYTAAEVLGRSLDFLLPDAGAGRAGEAARVSLVEFLERCATK
                     IDDTHVDLAAHQTWGTTKDRVRVEVEVAVSKASLNRRDGYIVCIRDCTVDITERKRGD
                     AIAAGERRVFEKIAANAPLPSVLEAIAEVIERVVEGSFCAINLLDQEDQMIRFGAAPR
                     LPRAFVAAMDDAPVGIRFGSCAAAAYLSRQVTVADIENDALWEYRREAARHAGFRAAW
                     SAPIIASDGIIVGTFSAYQRQPGMPLSRDHELMLRMSQLAGIAIERRSAEDALRDSEA
                     KFRGLFESVMEGVYRTARDGRFLVVNPAFVQMLGYGSAEEIYQLHAASLYWYPTDRDS
                     YVRRLDTEGEVRNVEYVLRRKDGSMLVVVDNGRVVRDKEGRISGFEGTIADITERKRA
                     ETAVFQAKERAQVTLQSIGDAVITTDSEGCIDYMNPVAESLTGWESREAQSQRIGDIL
                     TVVDEATRKPAESPVMRCLREGRPLGLSEHTVLINRRGQEIAIQDSAAPIRDRAGNML
                     GAVMVFHDVSKERRLHRALHYQASHDALTGLINRREFDNRLTAAVDSVRSDPESRHAL
                     LYLDLDQFKLINDTCGHPAGDQLLKQITGVLQSRVRLGDTLARLGGDEFGILLQDCPV
                     DQALRVAEGLRQAIRDYRFVWQDGVSAVGVSIGIVEITRETPTVAGVMSAADVACYSA
                     K"
BASE COUNT         1367 a         2334 c         2501 g         1376 t
ORIGIN      
        1 ggcgcagcgg aaggcggacc cggaagtgag cgcctcccgt ctttgcctcg aaaatctcga
       61 tgccgccgcc atgggcgttc acgaattccg tcaccaccga gaggccgatc ccggtgcctt
      121 tgacgtggcc cccctgcggg gtcttgccct ggtagaacgc ctcgaatatg cggttgcgat
      181 cttcagccgg aatgccgggt ccgctatcca tgacatccaa tatcaattgc tcgcgttcgc
      241 cccgcgcgtg aatcgagatg gtgccccctc gaggggtgaa tttgatggca ttcgacaaaa
      301 gattatcgag tatgagacgc accttgcccc gatcggcgca cggggtgaga tcctcgacct
      361 ggacgtcgag ccggacgcgc tgcgccacca gcgtcagctg ctggttttcg aggactgatt
      421 tcaccaatgg acgcaacttg aactccgtga tctcgaggcc gacgctcttc gcctgccagg
      481 cgctgaaaga gagcaaattt tcgatgagcc gctgcaattt catcccgttc tcgcgcaaga
      541 tcgccatcac ttcgcgctgg gcgctctgga gttcaccgac cgctccgtcc atcaggagtt
      601 cggttccttc gcgaatattc gcgagcgggg tcttcaattc atgcgacatg tgtcgcagga
      661 atcgattctt ttcctgggcc agctccaaca atctgccgcg cagccattcg agctgggcgg
      721 cgaggcgctc cagatcggtc ggtccgctga tggcgatcgg cctggaaaac gtgccgcggc
      781 cgagctcgct gatggcgcgg tcgatcgccc tgatcggtcg gccgaacagg taggtgaaaa
      841 agccaaccgc ggcgaccgtc acgggtacca gcagcagcgt ccaccagaac agcttttgct
      901 gcgcccgttg ggcggcaagt tgcagactgg tcagctcgtc gttgatgtcg gtgttgatgc
      961 ggttcgacag ggtcgaggcc atctcggaca tgtgcggaaa ggcgttgatc gtctccgcca
     1021 tccggggcga gttcggcggc gcggccttca gttcgcgcga cagccggtcg gcttccgcaa
     1081 gcaggccgcg ggcatcgacg tgcgattgct catcgagcgg caacgtcagg agttctccga
     1141 tcgtcgctgc caggcgtgcc tggttgcggg tgtagccttc gagcagctcg gtgttgccga
     1201 tgatctgata aagccgcgcc gaacgctcca gcgcaccgat ctcctcgaac atgctttggt
     1261 tgttgcgggt accctggacg ccatggatga ccaattgttg actgcggttc gagaggcgat
     1321 tcatctgcac ggcggcgttc acgatggcga acagcagcgg tgcggcgacg gtcgtgaagc
     1381 cgatcagcat cagtccgctc agcgatttgg gcctgggtag tcgcatgcca ttcattctaa
     1441 catcgcaccc atgaccgccg aggcacctta ttccgacctg tctcccgaga tcgtgctgga
     1501 tgcgctcgag gcggcgggtc atcgctgcga cggacgagtc ctcgcactca acagctatga
     1561 gaatcgcgta taccagatcg gtaccgagga tgcggagccg gtggtcgcaa aattctatcg
     1621 gcccggccgc tggaccgatg ccgccatcct cgaggaacac gccttttcac tggaattggc
     1681 cgcgcaggaa attccggtgg tggcgcccct ggcgcgcccc gccgaggccg gcggccagga
     1741 atcgctgttc gtccaccgcg ggttccgcta cgccattttc ccgcggcggg gaggccgctg
     1801 gcccgagttg gcgaccgcaa ccgaccgcga gtgggtgggg cggtttctcg ggcgaattca
     1861 tggcgtcggc cgcgcggacc ggttcgttca ccgcgggcga ttgagcgtgg cggaactggg
     1921 gcgcaacgcg cgggatttcc tgctggaagg cgattggatg cccgactatc tcgcaacgaa
     1981 gtacgaagac ctcaccgact cgctgttgga ggaagtcgag gcgcaggcgg acgggtgggg
     2041 aggcgcgcgc ctcggacgga tcctcggcga ttgccaccgc ggcaacatcc tctggaccga
     2101 tctcggaccg catttcgtcg atttggatga ttgcctcacg ggacctgcaa ttcaggacgt
     2161 ctggatgctg cttgccggca gtcaacagga gatgcggacg gatctgaccg atctgttgag
     2221 aggctacgag cagtttctgc cgttcgaccg gggcgagctg gctctcatcg agccgctgcg
     2281 ggcgctgcga atgattcatt actcggcctg gctggcgcgg cgctggcacg atccggcatt
     2341 tccccgggca ttcccgtggt tcgccgagcc gcgctactgg gaagagcact accgcgcgct
     2401 cgaggagcag ctgggcgccg tgttggggcc gcggctggag ttgtgaatgc ctgccgggag
     2461 ccgcggcggc cggagaggcg attgcctgcc ttgcgccgcg gcggccggag aggcgattgc
     2521 ctgccttgcg ccgcgtccgc gcggccggag ttgtgattgc ctccgtgttt gagggattat
     2581 gggggatgac ccggggagcc cgtctgagtc tttctcgtga gccgcgttgg gcgctgcttg
     2641 cggccgtgct gttggccgcc tgccatggcg aaccgaaaca ggcgccggag gccgctcccg
     2701 cgacggccgc gagcgctcct gccgtggcca aacgaggccc gacgcctgag gagttgaccg
     2761 cgggcatggt cgaagcggtg accatcggca agtcgaccgc gccggtcacc atcaagttcg
     2821 acctgccgca acggccttcg gtgggcaagc gcatggagat cgtcattgcg gtcctgccgc
     2881 aaattgtcgc gagctccgcg atggtgcagg tcgcaggttc agaagggctg cagctcgccc
     2941 cgaacttcgc acccatcgaa atcgcttcgc ttgaccctac ccaggtgtac cggctgagcg
     3001 ttccggtgac cccgaccgcc gatggcgtgc agctgctcgg cctcaacgtg tccttgaaag
     3061 tggacgattc ggcggaatcg cgtacttttt cggtgccgat catcgtctcg cccggcggaa
     3121 gcgactgaaa cttaaggcgg aacttaaggc ggactggcga aacttgaggc ggatggggcg
     3181 ataagactct ataatggtgg gtttcgctta ggactccact gtgacgcaga aactccgcaa
     3241 catcgccatt atcgcgcacg tcgatcatgg caagaccaca ctcgtcgacc agctcctgcg
     3301 tcaatcgggg acgcttgatg cgcgcaagga tctccaagag cgcgtcatgg actcgaacgc
     3361 gctggagcgc gagcgcggca tcaccatcct cgccaagaac acggcgatca cctggggcga
     3421 tttccggata aacatcgtcg atacgccggg gcatgcggat ttcgggggag aggtggagcg
     3481 ggtgctcgcg atggtggatt gcgtgctgct gctcgtggac gccgtcgacg gacccatgcc
     3541 tcagacgcgc ttcgtcactc agaaggcatt caaacacggc ctgcgtccca ttgtcgtcat
     3601 caacaagatc gatcgggacg aagcccgccc gggctgggtg ttggaccaga ccttcgatct
     3661 gttcgaccgc ctcggggcga ccgacgagca gctggatttc cccgtggtgt acgcatcggc
     3721 attgcgcgga ttcgcgggtc tcgactcgac cgttcgcgac ggcgacatgc agccgctgtt
     3781 caagaccatc gtggaccatt gcccgccccc ggacgtcgac tccgacggtc cgctgcagtt
     3841 gcaggtgacc ttgctggact attccagcta cgtaggtgcg atcgggatcg gccgcatcaa
     3901 gcgcggcacc ttgcgcaaag gcatggcggt cacggtggtc aaccgagagg gcaagcagcg
     3961 ctcggaaaga atcacccagg tattggggtt ccacggactg acccgaatcg aggtcgaatc
     4021 ggcgtttgcg ggcgatatcg tggcatttgc ggggattgcc gagcccaagg tatcggacac
     4081 cctctgcgat ccgctgaatg tcgaagcatt gccgaatctg acggtcgacg aaccgaccat
     4141 cagcatgacg ttcgaggtca atacgtcgcc gttcctcggc cgcgacggta aattcgtgac
     4201 cagccgccag atccgcgagc ggctcgaccg cgagtccata cacaacgtgg cgttgcgcgt
     4261 cgaaccgacg tcggatccgg acaagttcgt ggtgttcggc cgcggcgaat tgcacctcgg
     4321 ggtgttgctg gagaacatgc ggcgcgaagg ctacgaaatg gctgtttcca ggccgcgggt
     4381 ggtcatcaag cacgtcgacg gcgtagccca tgagccgtac gagcaggtga ccgtcgacgc
     4441 cgacagcgac gcacagggtt cgatcatgtc ggcactgggc agccgcggcg ccgaactcaa
     4501 ggacatgcag gccgacggcc ggggccgggt ccgcctcgat tacatggttc cctctcgcgg
     4561 cctcatcggg tttcaaacgg agttccgcac catgacccgc ggaaccgggt tgctgcatca
     4621 tgtgttcgat cactatggtc cggtggtgca acgggaactc ggtcagcggc cgaacggggt
     4681 tttgatcgcc aatgggcagg gggtggcgct gggctattca ctgttcagtc tgcaggagcg
     4741 gggcaggctg tgtatcggcc ccggcgaaga ggtttacgaa gggatgatca tcggtctcaa
     4801 caatcgcgac gacgatctcg tggtcaatcc tctcaagggc aagaaactca ccaatatgcg
     4861 agccgcgggc aaggacgaga acgtcgttct cacgccgccc atccgcttct ccctggagca
     4921 ggcgatggag ttcatcgatg acgacgaatt gatcgaagtg acgccgaatt cgatccggct
     4981 gcgcaagcgc aacttgcgcg gaagcgaccg caagcggtcg gggcgcgaaa ccgtggatca
     5041 ggcgtcctga ccggtcgagg ataatttaac cgaatattga ccgaaccgag acgcccgtca
     5101 cgccgccgct gcgggatctc cctaacattc gcgactgagg gcgggatgcc cttcaagttt
     5161 cagtgcggga ggtttcagga gtggcacgcg acattgcgac cgcgctgcgc agcgcggtgg
     5221 cggaaacccc cgacagctat aatcgcgtgc tcgaccagca gatcgaggct gcgcgcggtc
     5281 gcggcggcga gctcgacctg gactctctgt tcaagcaggt cagcgcccat tatgaccgtc
     5341 tcgatgccga gcggcgcggc gtcgtgctct ccatgcaact catgtcggac gaggctcagg
     5401 cgctcacccg cgagatccgt gaagaaacgg cctcgcactt gcaggccatt ctggacaacg
     5461 tcaaggatgc catcgtcacg gtcgacgatg gcggccacat cgaaacattc aatcccaccg
     5521 gagaacgtct tttcggctat accgccgccg aggtcttggg ccgcagtctg gatttcctgc
     5581 tgcccgatgc gggtgccggc cgggccggcg aggctgcgcg cgtctcgctc gtcgagtttc
     5641 tcgagcgctg cgccacgaag atcgacgaca cgcacgtcga cctggccgcc caccagacct
     5701 ggggcaccac caaggaccgg gtgcgggtag aggtcgaggt cgcggtcagc aaggcgagcc
     5761 tcaaccggcg cgatggctac atcgtttgta tccgcgattg cacggtcgat atcaccgagc
     5821 gcaagcgggg ggacgccatc gcggcaggcg agcggcgggt gttcgaaaaa atcgccgcca
     5881 atgcgccgct cccgtcggtt ctcgaggcga tcgccgaggt catcgaacgc gtcgtcgaag
     5941 gcagcttctg cgccatcaat ctgctcgacc aggaagacca gatgatccgc tttggcgccg
     6001 cgccgcggct gccgcgagcg ttcgtcgccg ccatggatga tgcgccggtc ggcatccgct
     6061 tcggttcgtg cgcggccgcc gcctatctct cccgccaggt caccgtggcc gacatcgaaa
     6121 acgacgctct gtgggaatat cgccgcgaag ccgccaggca cgcgggcttc cgtgccgcgt
     6181 ggtccgcgcc catcattgca tcggacggga tcatcgtggg gacattttcg gcataccagc
     6241 gccagcccgg catgccgctg tcgcgcgatc acgagttgat gctgcgcatg tcacagctcg
     6301 ccggcatcgc gatcgaacgg cgcagcgccg aggacgctct acgcgacagc gaggcgaaat
     6361 tccgggggct gttcgagagc gtgatggagg gcgtctatcg aacggcccgc gacgggcgat
     6421 tcttggtggt gaatccagcc ttcgtgcaga tgttgggata cggctcggcc gaggagatct
     6481 accagctgca tgcggcctct ttgtattggt atccgaccga tcgcgacagc tacgtccgcc
     6541 ggctcgatac tgaaggagaa gttcgcaacg tcgagtacgt attgcgccgc aaggacggct
     6601 cgatgctggt ggtcgtcgac aacggccggg tggtgcgcga caaggagggc cgaatcagcg
     6661 gattcgaggg caccatcgcc gacatcaccg agagaaagag ggccgaaacc gcggtcttcc
     6721 aggcgaaaga gcgcgctcag gttacgctcc aatcgatcgg cgatgcggtc atcaccaccg
     6781 actccgaagg gtgcatcgat tacatgaatc cggtggcgga gagtctgacc ggttgggaga
     6841 gccgggaggc gcagtcacag cgcatcggcg acatcttgac cgtggtcgac gaagcgacgc
     6901 gcaagccggc ggagagcccc gtcatgcgct gccttcgcga gggacggccg cttggcttgt
     6961 ctgagcatac cgtgctcatc aatcgccgcg gacaggaaat cgccatccag gattcggcgg
     7021 caccgatccg cgaccgcgcg ggcaacatgc tcggcgccgt catggtgttt cacgacgtca
     7081 gcaaggaacg gcgtctccac cgcgcgctgc attatcaggc gagccacgac gcgctcaccg
     7141 ggctcatcaa ccgccgcgag ttcgacaatc ggctcaccgc ggcggtcgac agcgtgcgct
     7201 ccgaccccga aagccggcac gcgctgctgt atctggatct cgatcaattc aagctgatca
     7261 acgacacatg cggccacccg gcgggggacc agctcctgaa acagatcacg ggggtgctgc
     7321 aatcgcgcgt gcgccttggc gatacgctgg cgcgactcgg aggagacgaa ttcggcatcc
     7381 tgctgcagga ttgcccggtc gatcaggcac tgcgcgtggc cgagggcctg cggcaggcca
     7441 tccgcgatta ccggttcgtc tggcaggacg gcgtatcggc ggtcggcgtc agcatcggca
     7501 tcgttgaaat cacgcgtgag acgcccacag tcgccggcgt gatgagcgcc gcggacgtgg
     7561 cctgctattc cgcgaagg
//
LOCUS       DATGEM010000201         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1688322, whole genome shotgun sequence.
ACCESSION   DATGEM010000201 DATGEM010000000
VERSION     DATGEM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688322"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VKQ27_06450"
     CDS             <1..1153
                     /locus_tag="VKQ27_06450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLY88607.1"
                     /translation="WAQRWVDSGKLPGLLTMVARHGKIVHLNACGMADVKREVRAAPD
                     TIYRFYSMTKPLTSTAIMMLYEEGHFQLDDPISRFLPCFANSRVAAGGMRGKIETVPA
                     ERDITFRDLLTHTSGLTYGFMEATPVDAQYRDRGVDFQTSDKTLGEVVEIAAGIPLIA
                     QPGAEWNYSIATDVLGHLVAVISGKQFAEFLRERVTGPLGMVDTDFHVPTDKLPRFAG
                     NYTRAADGRLMLIDDPQKSIFGQKRQIASGGGGLTSTASDYMRFCQMMLNKGELNGVR
                     LLGRKTVELMMTNHLRGDMAAMGQARFSESSYEGIGFGLGFSVTLDPARAQILGSAGE
                     CAWGGAASTAFWIDPAEDMAVIMLTQLMPSSTYPIRRELRVLTYQAVVD"
     gene            complement(1166..>2500)
                     /locus_tag="VKQ27_06455"
     CDS             complement(1166..>2500)
                     /locus_tag="VKQ27_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88608.1"
                     /translation="VAVLIPGAVLVTLAVIAWPLALALLPFLAYAAGSPIFGRRRIDT
                     LASAARRALGELGAHATETIQGLAELMAFEATSRRRDEFMLYVRQYQEMRLHLLHDLS
                     RQTAGLEMATGLGGLAVAAVGAPLVLHGWIAPTMLPLLVLISIAAFLPVSEIAQVGRQ
                     LADTIASTRRLHVVHAEPVVITDGPRVPASPANGSTVTFEHVRFTYPDRARSALADVD
                     FTVPAGATVALVGPSGAGKTTIANLLLRFWDPDQGGIKLDGVDLRELTLDGLRGRIAL
                     VAQDTYLFNNTLEANVRLARPDATREQIETALRQAALSDFVGGLPDGLATRVGERGVQ
                     LSGGQRQRIAIARAFLKDAPILVLDEATSHLDTISEAQVREALDKLMVNRTTIVVAHR
                     LSTIQAADIILVLNAGRVLEAGTHVELSARRGFYARLVEHQMAGLLDRAAAN"
BASE COUNT          453 a          783 c          814 g          450 t
ORIGIN      
        1 ctgggcgcag cgctgggtgg acagcggcaa gctgccgggc ctgcttacga tggtcgcgcg
       61 tcatggtaag atcgtgcatc tcaacgcctg cggcatggcg gacgtgaagc gcgaagtccg
      121 ggctgcgccg gacacgatct atcgcttcta ttcgatgacc aagccgctca cctcgacagc
      181 gatcatgatg ctttacgagg aaggacattt tcagcttgat gatcccatct cgaggtttct
      241 gccgtgcttt gcgaattcgc gcgtggcggc gggcggcatg cgcggcaaga tcgagacggt
      301 gccggcggaa cgcgacatca ctttccgcga tctgctgacg catacgtccg ggctgaccta
      361 cggcttcatg gaagcaacgc cggtcgatgc gcagtaccgt gatcgcggtg tcgatttcca
      421 gacatcggat aagacgctgg gcgaggtcgt ggagattgcg gccggcattc cgctgattgc
      481 ccaacctgga gcggagtgga actacagtat cgcgacggat gttctcggac atctggtcgc
      541 ggtgatttca ggcaagcagt tcgccgagtt cctgcgtgag agggtcactg gaccgctggg
      601 catggtcgat accgatttcc atgttccgac tgacaagctg ccgcggtttg cggggaacta
      661 cacgcgcgcc gccgacggca ggctgatgct gatcgacgat ccgcagaaga gcattttcgg
      721 ccaaaaaagg cagattgcgt cggggggagg cggcctgact tcgaccgcct ccgattatat
      781 gcggttctgc cagatgatgc tgaacaaggg cgaactcaac ggcgtacgct tgttggggcg
      841 gaagacggtc gaactgatga tgaccaacca tctgcgcggt gacatggctg cgatgggtca
      901 ggcccgtttc tccgagtcgt cgtacgaggg gattggcttc ggccttggct tctcggtgac
      961 gcttgatccg gcccgcgcgc agatccttgg ctcggccggt gaatgtgcct ggggcggcgc
     1021 cgccagtacc gcgttctgga tcgatccggc ggaggatatg gcggtcatca tgctgactca
     1081 gctgatgccc tcatcgacct atccgatccg gcgggaacta cgggtactga cttatcaggc
     1141 cgtggtggac tgaggcggcg ctccctcagt tcgccgccgc acggtccagc aggcctgcca
     1201 tctggtgctc gacgagccgc gcgtagaatc cacgccgtgc gctcagttcc acgtgcgtgc
     1261 ccgcctcgag cacgcggcca gcgtttagta ccaggatgat gtccgccgcc tgtatggtgg
     1321 acaaccgatg cgccaccaca atcgtcgtgc ggttcaccat cagcttgtcg agcgcctcgc
     1381 gcacctgagc ttcgctgatg gtatcgagat gcgaggttgc ctcatccagc acgaggatcg
     1441 gcgcatcctt caggaaggcg cgtgcgatcg cgatgcgttg ccgctggcca ccggaaagct
     1501 gcacgccgcg ctcgccgaca cgcgttgcca gcccgtccgg caggccgccg acgaagtcgg
     1561 acagcgctgc ctggcgcagt gccgtctcga tctgctcacg tgttgcgtcg ggccgtgcga
     1621 gacgcacatt cgcttccaag gtgttgttga acaggtaggt gtcctgcgcc accagagcga
     1681 tgcgtccacg caggccatcc agtgtcagct cgcgcagatc gacgccatcc agcttgatgc
     1741 cgccctgatc gggatcccag aagcgcagca acaggttggc gatcgtggtt ttgccggcgc
     1801 cggatgggcc aaccagcgcc accgtcgcac cggcgggcac ggtgaaatcc acgtccgcca
     1861 gagcggagcg cgcacggtct ggataggtga accgtacatg ctcgaacgtt acggttgagc
     1921 cgttggccgg agaggctggc acgcgtggac cgtccgtgat cacgacgggc tcggcatgca
     1981 ccacatgcag ccggcgcgtg gatgcaatcg tatcggcaag ctggcggcca acctgagcga
     2041 tctcggacac cggcaggaat gcggcgatcg atatcagcac cagcagcggc agcatcgtcg
     2101 gcgcgatcca gccatgcaac accaacggtg cgccgacggc cgcgactgcc aggccaccga
     2161 ggccggtggc catttcgagg cccgccgtct gtcgcgacag gtcatgcagc aggtgaaggc
     2221 gcatctcctg atattggcgc acatacagca taaactcatc gcggcggcgg ctcgtcgcct
     2281 caaacgccat cagttccgcc agcccctgaa tcgtctcagt ggcgtgtgcg ccaagttcgc
     2341 ctaacgccct gcgcgccgcg gaagccagcg tatcaatgcg ccgccgcccg aatatcggcg
     2401 acccagctgc gtatgccagg aacggcagca gcgccagcgc cagcggccag gcgatcacag
     2461 ccagcgtcac cagcacggct cctgggatca gcaccgcgac
//
LOCUS       DATGEM010000202        13302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1229964, whole genome shotgun sequence.
ACCESSION   DATGEM010000202 DATGEM010000000
VERSION     DATGEM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229964"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VKQ27_06460"
     CDS             complement(<1..1733)
                     /locus_tag="VKQ27_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HLY88609.1"
                     /translation="MPDARFASLTPLFAPRSVAVLGASSDPTRISGRPIAYMKAQGFP
                     GGLYPINPNRTEIQGLKAYPSVADLPETPDVAIVAVAAEIAEKSITDLAERGVKAVVM
                     FTAGFAEMDEAGEAAQNRMVAVARKHGMRILGPNCLGVFDARTAYYATFSSSFDSGWP
                     VPGRIGIASQSGAYGTHLYTLARNRGIGASLCIMTGNESDVTVGECIGWLAENPEVDV
                     IAVYAEGIREAPGLIAAFEAARAAKKPIIMQKVGRSELGSKAAKSHTASIAGDDAVTE
                     AVMAEFGVVRARNSEEMLDIAHTATRKIYPARNTLGVITVSGGAGVLISDTADAVGLP
                     MPEMPADTQKTLRELVPFCAPRNPVDATAQVSNTVELIHTFTEALVKDGGYSSVLGFF
                     SMTASSRRWPGIREQLNKVREAYPGRLYTLSVIVPPDRRAELEGDGWVVHEDPTRAVT
                     AIAAMGRFGAAFDAPAGAGAPSVPAVTLPDSTPSEAEAKRLLAAAGIASAPEQACASA
                     DEAVAAAERFGFPVVMKILSPDILHKSEIGGVLLNVGDAAAVRDGYGVLLSRAKQAAP
                     SARIEGVLVAK"
     gene            1922..2872
                     /locus_tag="VKQ27_06465"
     CDS             1922..2872
                     /locus_tag="VKQ27_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY88610.1"
                     /translation="MVLTLSHFLGPSSFFQRDFAEPWARKLAAASNGAVRIEIYNNTS
                     LYGDVTKQARQVEDGDVDIALGLCGAEDDRFPASSIIELPFAVNNALDGSRALWKLYK
                     DGELGNDYSGFKVLALFVHNPGLIHTAVKRITNPGDLKDRRLRAPNATVAAALKSIGA
                     APAVLQVNDVMPAVKDGTIDGIVTNWGNPLPDFNRYMKHHLDIAFYTSAFFVVMNQRR
                     FDGLPPDIQAAIDKVSNEWLVTQFGLLWNRWDRPVREGATGPDQQILIPDPTLLAEWR
                     NALQPFTERNLDSLIASGFTGARTAHARLLRFSSSLAPSR"
     gene            complement(2882..4039)
                     /locus_tag="VKQ27_06470"
     CDS             complement(2882..4039)
                     /locus_tag="VKQ27_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLY88611.1"
                     /translation="MSRPHRSDNRMVSYAALLRGLGCITLLAMLAACGSQSQQPSATQ
                     EAARYLAHARSNYAPPGPPEDPWGPYIREATKRYDVPEIWIRSVMRVESGGNQYQGGR
                     LTMSSAGAMGLMQVMPETYQELQSRYSLGDDPFDPHDNIMAGAAYLREMYDIYGSPGF
                     LAAYNAGPRRLDDYLSNNRPLPDETRRYVAMIGPNVVGVYPNNRSPAEAYAMNALPID
                     IPPGPRYGRAVQLASSRGRGGRLPARAPVEMAALPEPPRATSQVQQAQYALVAPPTPP
                     HGRFHLISQAVAAEAVPLRRGGGSTGEWAIQVGAYSSEGLAHAALVTARQHAHEELAV
                     AHVFVAGVHQGRATLYRARLTGLSREAAVGACERLSHGRTSCMVLSPDSQL"
     gene            4377..4652
                     /locus_tag="VKQ27_06475"
     CDS             4377..4652
                     /locus_tag="VKQ27_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88612.1"
                     /translation="MRPSTPFSAATILSMRAIDTSAGSAPWRAALLAAASEGSGGSTS
                     PAPLTEFIVRADDGATLSIVQSNKLDLHTGERVVIRYEPTVQLARPG"
     gene            4734..5558
                     /locus_tag="VKQ27_06480"
     CDS             4734..5558
                     /locus_tag="VKQ27_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88613.1"
                     /translation="MTVLEFRGDMSAATRNRLALLDIVEGIALWRLAWSLGWLDIRLR
                     YRGSILGPFWLTLSTGVMIGSLGVLYSALFHIDTRNYLPFLALSQVLWGFIATLVGEA
                     CAAFTQAEGMIRAVRMPLFVHAIRTLLRNVFVLAHTIVVIVVVDVIFSVSPGWHAPLA
                     LPGVVLWMFDGLAICLLLGAFCARFRDIAPIVASIMQIAFFLTPVIWKPEQLGGHAWL
                     LPLNPFFAIMEIVRAPLLGTSPSHTTWLAAIVYSVVLFALSWLMFVKARGRVAFWM"
     gene            5561..6343
                     /locus_tag="VKQ27_06485"
     CDS             5561..6343
                     /locus_tag="VKQ27_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88614.1"
                     /translation="MSSARIDITDVSVFFPLYHGSSRSLKKTVMAAASGRLGQDRQSR
                     VVVQALRDISFSLQSGDRLGLVGANGAGKTTLLRTLGGIYEPVTGQVRLQGSVTTLLD
                     TTLGMNLELTGRENIQLRGLYTGVARTDIPAMEEDVAQFAGLGDFIDLPVRTYSSGMV
                     VRLAFAMATALRPQILLMDEWFIVGDAEFMEKARDRLEGMVRGAEIMVLSTHALEIIR
                     SWCTRVLWIDQGRIREDGSPDEVIAAYLGHPLPAPAPAPAAT"
     gene            6359..7537
                     /locus_tag="VKQ27_06490"
     CDS             6359..7537
                     /locus_tag="VKQ27_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY88615.1"
                     /translation="MAILPLADLTVLEFSHTVMGPSCGVMLADLGADVIRVEPAPDGD
                     RTRRLHGFATGFFAYFNRNKRCICIDLKSPDGQAVAHDLLGKADVLIENFGPGTMDRL
                     GLGWDTVHALNPRLIYCALKGFLPGPYEHRPSLDEIAQYMTGLAYMTGPPGQPLRAGS
                     SVIDIMGGVMGAMAILAALRQRDKDGIGRKVTSALYESSAFLVGQHMAGEAATGTPSP
                     PMPARSGAWGIYETFPTLNGEKLFIGITSDNHWRSFCAMFDRPDLLADERFTTNADRV
                     RNRPPLRDIVAAIASQHAIADLERMMDAANIPFSPVRTPSDLFDDPQLNAFGRALAVR
                     MPKGNVANLPSLPFCIDDEAPALRMQPPAAGEHSDAILATLGYSAEQIAKLRDARTIA
                     "
     gene            complement(7669..9174)
                     /locus_tag="VKQ27_06495"
     CDS             complement(7669..9174)
                     /locus_tag="VKQ27_06495"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HLY88616.1"
                     /translation="MSGAARFPTSPGLSAPGGGEGDQAVAARSGEQFLRGLNDDREVW
                     LGRERISDAATHPALKGAAHSIAAVFDLQHERADVCLMPDPETGEPINVSHMIPRSRE
                     DLVRRHAGLQAIAEYTVGLMGRTPDYVNVTYAGFAGCHGEWSHSGNERGAENLIRYQK
                     HMARADLSLTHAIIHPTTDRARGAVPLGVDNVSLHKVCDTEHGIVVRGARVLATLAPF
                     ADDVAVYPAAPMPDANPTHALSFSIPMATPGLKFLCRDSVSVPGTLFDNPFSSRFDEQ
                     DAFVIFDNVEVPRDRVFIDGNLAVYNGILRSSWWPNIMQQAMIRAETKLTFAWGIASR
                     MAEAINASLPPALQMLGEIWTFAQFARSSLRAAEVDAREYGDGVWFPAVEPLIALRAS
                     LPRWFPRVNEIIRELGSHNLLTTPTEAQFANPALRPVLDEMLGGAGEMGAEERARIFR
                     LAWDFAGSALASRNEQYERFYLGSGSRNLMHAHQRGDRQRADRLVDRFLNE"
     gene            complement(9209..10123)
                     /locus_tag="VKQ27_06500"
     CDS             complement(9209..10123)
                     /locus_tag="VKQ27_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLY88617.1"
                     /translation="MTDALVPESPAEVQAAVGYERPETLAWDSKLRRTVTIYIPLALF
                     VIVLLFPFYWMAITSIKPDDEMYDYHRFNPFWVHSPTLHNIEVLLFQTNYPRWLMTTM
                     GIAVAATFLSVGASVLAAYAIERLKFKGAQHVGLAIYLAYLVPPSILFIPLATLIYQF
                     GLFDNPVALVLTYPTFLVPFCTWLLIGYFKSIPYELEECALIDGASRLQILRRITLPL
                     AVPGLISAGIFAFTLSWNEFIYALAFIQSTDKKTVPVAILTELVTGDVYQWGALMAGS
                     LLGSVPVALIYSFFVEYYVSSMTGAVKE"
     gene            complement(10116..11084)
                     /locus_tag="VKQ27_06505"
     CDS             complement(10116..11084)
                     /locus_tag="VKQ27_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLY88618.1"
                     /translation="MGTTTADVGLQRLTFTRKPDGWLTRLFDNKSFLVFLCMLPTIGL
                     LVVFLTYPLGLGLWLSMTDATIGQPGQFIGLDNFVDLMSDPIFWMSVTNTIFYTTVAT
                     IGKFALGLWLALLLNHHIPFKAFIRAIVLLPWIVPTVLSAIAWWWIYSPQFSIISYVV
                     VDVLHLRDTYINFLGTPWPARWSLIVANIWRGIPFVAITLLAGLQTISPSLYEAALLD
                     GASGWQKFRRITVPLLMPILAVALTLSVLFTFSDFQLVYAITRGGPFNTTHLMATLAF
                     QRAIPGAQLGEGAAIAVSMIPFLIGATLLCFFGVGRRKWQQGEAND"
     gene            complement(11145..12491)
                     /locus_tag="VKQ27_06510"
     CDS             complement(11145..12491)
                     /locus_tag="VKQ27_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY88619.1"
                     /translation="MTISRRNSLALGAAALGAASIPMIGARAAFDDVPTADVKPPEFK
                     IEKGASLHMIRPAKFIDPDEVYWKKNTEEFTKKTGVEVKVDFISWEDLRPQLAVVANT
                     GAGADIFVSFGADSHIYSEKLVEMTDLADYLGAKYGGWYPLALLYGQKWKTKNWIALP
                     LGGGTGPVLYRKSWASEAGYNEIPNDLEKFLDLCQKMQKAGHPWGMSLGHALGDANGY
                     SEWLLWSHNAYVVDEAGKVALDSKETIAALKYATELQKTMIPGTLTWNDVGNNQAYQS
                     GAIGMTFNGVSLYYSMLKSPDQKVQAMAADTFTQDTPYGLSKQKPMSAAPISAMLFKH
                     SKYPNAAKEYIRFMMEKDQYGLWLSSCLGYWCQPLKAYSKMNFWNSDPKLKPFATALD
                     SPFYDGYKGPISAASAAVIANYTVVDMFASVVSGNATPESAAKQAAKAAERYYRKA"
     gene            complement(12593..>13302)
                     /locus_tag="VKQ27_06515"
     CDS             complement(12593..>13302)
                     /locus_tag="VKQ27_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HLY88620.1"
                     /translation="RAAQILGLTQYLNRYPRQLSGGQRQRVAMGRAIVRNPQVFLFDE
                     PLSNLDAKLRVAMRAEIKELHQRLETTTVYVTHDQIEAMTMADKIVVMNSGNVEQIGA
                     PLELYDRPANLFVAGFIGSPAMNFLQGKIEGGAFHAGGEALPLPNGGKSSDGRPAVYG
                     VRPEHFQLSPGGFPAKVHVVEPTGSETQVVAEFAGASVVCAFRERISAKPGETIRIAP
                     DPALVHLFDAATGQRAG"
BASE COUNT         2477 a         4535 c         3886 g         2404 t
ORIGIN      
        1 tgcttcgcta ccagcacgcc ctcgatgcgc gcactcggcg cagcctgctt cgctcgcgac
       61 aacagcaccc catatccatc gcgtaccgcc gctgcatcgc caacgttcag cagcacgccg
      121 ccgatctccg acttgtgcag gatatcgggc gacaggatct tcatcaccac cgggaagccg
      181 aaccgttctg ccgccgccac cgcctcatcg gcgctcgcgc aagcctgttc cggcgccgaa
      241 gcgatgcccg ccgcagcaag cagccgcttc gcctccgcct cgctcggcgt gctgtccggc
      301 agtgtgaccg ccgggaccga aggcgccccc gcccccgccg gcgcatcgaa tgcggcgccg
      361 aaccgtccca tcgctgcgat cgccgtcacc gcacgcgtcg gatcctcatg caccacccag
      421 ccatcgcctt ccagctccgc gcgacgatcg ggcggcacga tcaccgacaa cgtgtatagc
      481 cgtccgggat atgcctcgcg caccttgttg agctgctcgc gaatgccagg ccagcggcgc
      541 gacgaggcag tcatgctgaa gaagccaaga accgacgaat acccgccatc cttcaccagt
      601 gcctcggtga aggtgtggat cagttccacg gtattcgata cctgtgccgt cgcatccacc
      661 ggattgcgcg gcgcacagaa cggcaccagc tcacgcagcg tcttctgcgt gtcagccggc
      721 atctccggca tcggcagccc caccgcgtcg gcggtatcgc tgatcagcac gccagcgcca
      781 cccgacaccg tgatcacccc cagcgtattg cgcgcgggat agatcttgcg cgtcgcggta
      841 tgcgctatgt ccagcatctc ctcggagttg cgcgcgcgca ccacgccgaa ctccgccatc
      901 accgcttcgg tcaccgcatc gtcgcctgcg atcgatgctg tgtgcgactt cgctgccttg
      961 ctgccgagct cgctgcgccc gaccttctgc atgatgatcg gtttcttggc cgcgcgcgcc
     1021 gcctcgaacg cggcgatcaa tccaggcgcc tcgcgaatac cctcggcgta gaccgcgatc
     1081 acatcgactt ccggattttc agccagccag ccgatgcact cgcccacagt cacgtcggat
     1141 tcgttgccgg tcatgatgca gagcgacgcg ccgatcccgc gattgcgcgc cagcgtgtag
     1201 agatgcgtac catacgcgcc tgactgcgag gcaatgccga tgcgcccagg cactggccac
     1261 ccgctgtcga acgacgatga gaacgtcgcg tagtaagcgg tgcgcgcatc gaacacaccc
     1321 aaacaattcg gcccaaggat gcgcatgcca tgtttgcgcg ccaccgccac catgcggttc
     1381 tgtgccgctt cacccgcttc atccatctca gcaaaaccgg cagtgaacat caccaccgcc
     1441 ttcacgccgc gttccgccag atcggtaatc gatttctcgg cgatctccgc tgccaccgcg
     1501 acgatcgcca catccggcgt ctccggcaga tcggcgacgg acggatacgc cttcagcccc
     1561 tgtatctcgg tgcggttcgg attgatagga tacagcccgc ctggaaatcc ctgcgccttc
     1621 atgtaggcga tcggccggcc gctgatccgg gtcggatcgc tggaggcccc cagcacggcg
     1681 acagagcgtg gcgcaaacag cggcgtcagg cttgcaaaac gagcgtcagg catgattgcg
     1741 gacattcccc tgagatgttt tcgtacggtc agtgtggtcc taggatgaca aggcggcaag
     1801 cgttgccgtt gcaaccagcc cgtgcgcaga atgaccggat gcgaaatagc cttgtcgcat
     1861 ccacatattt cgcgctgttc ctactggacg gcgagatgcg ccctgactct gtggatcaga
     1921 gatggtgctg acgctcagcc acttcctcgg ccccagcagc ttcttccagc gcgacttcgc
     1981 cgaaccatgg gctcgcaaat tggcagcagc cagcaacggc gccgttcgca tcgaaatcta
     2041 caacaacacc tcactctacg gcgacgtaac gaagcaggcc agacaggtcg aagatggcga
     2101 tgtcgatatc gcactcggcc tgtgcggggc cgaggatgat cgtttcccgg ccagttcgat
     2161 cattgagctg cctttcgccg tgaacaatgc gctcgacggc tcgcgtgccc tctggaagct
     2221 ctacaaagac ggcgaactgg gcaatgacta cagcggcttc aaggtcctcg cgctattcgt
     2281 tcacaacccc ggcctgatcc acactgcggt gaagcgcatc accaatccgg gtgacttgaa
     2341 ggatcggcgg ctccgcgcac ccaatgcgac ggtagcagca gcgctcaagt caatcggtgc
     2401 cgcgcctgcc gtcctgcagg tgaacgacgt gatgcccgcg gtgaaggatg gcacgataga
     2461 cgggatcgtc accaactggg gcaatccact gcccgacttc aatcgctaca tgaagcatca
     2521 cctcgatatc gccttctaca cgtcggcctt cttcgtcgtg atgaatcagc gtaggttcga
     2581 cggcctgccg ccggacatcc aggccgcgat cgacaaggtg tccaatgaat ggctggtcac
     2641 gcagttcggc ctgctgtgga accggtggga tcgtccggta agggagggcg ccaccggacc
     2701 ggatcagcag atactcattc ccgatcccac tctcctcgcg gagtggcgaa atgcgctaca
     2761 gcccttcacc gagcgtaatc tcgacagcct tatcgccagc ggcttcactg gcgcgcgcac
     2821 cgcccacgcc aggctgctga ggttcagctc gagcctggcg ccatcaagat agaggcggac
     2881 cctatagttg cgagtcgggc gatagcacca tgcagcttgt gcggccgtgc gacaggcgct
     2941 cgcacgctcc caccgcagcc tcacgcgaca gcccggtcag ccgcgcgcga tacagcgtcg
     3001 cgcgtccctg gtgcaccccg gcgacgaaga catgcgcgac ggcaagctcc tcatgcgcat
     3061 gctgacgcgc ggtcacgaga gccgcgtgcg cgaggccttc gctcgaatag gcgccgacct
     3121 ggatcgccca ttcgcccgtc gacccgccgc cgcgccgcag tgggactgcc tccgcagcga
     3181 ccgcttgcga gatcaggtgg aaccgcccat ggggcggcgt cgggggcgcc accaacgcgt
     3241 attgcgcctg ctgcacctgc gacgtcgctc gcggcggctc gggcagcgcc gccatctcga
     3301 ccggcgcacg cgccggcaga cgcccgccac gtccgcgact gctcgccagt tgcaccgcgc
     3361 ggccatagcg cggccccggc ggaatatcga tcggcagcgc gttcatcgca taggcctcgg
     3421 ccggcgaacg gttgttcgga tacacgccga ccacattcgg cccgatcatc gcaacatagc
     3481 ggcgcgtctc atccgggagc gggcgattgt tgctcaggta atcatccagc cgccgcggcc
     3541 ccgcgttgta cgccgccaga aagcctggcg aaccgtagat gtcgtacatc tcccgcagat
     3601 acgccgcgcc ggccattatg ttgtcgtgcg ggtcgaatgg atcatcaccg aggctgtagc
     3661 ggctctgcag ctcctgatag gtctcaggca tcacctgcat cagccccatc gcgcccgcgc
     3721 tcgacatcgt gagacggccg ccctggtact gattgccacc agactcgacc cgcatcaccg
     3781 agcggatcca gatctccggc acgtcgtacc gcttggtcgc ctcacggata tacggccccc
     3841 acggatcctc cggaggcccc ggcggcgcgt agttgctgcg cgcgtgtgcc agataccgcg
     3901 ccgcctcctg cgtggccgag ggctgttgcg actggctgcc acaggcggcg agcatcgcca
     3961 acagcgtgat acaccccaga ccgcgcaaca gagcggcata cgacaccata cggttgtcgg
     4021 aacggtgggg acgggacatc cgcaaaaact ccgtcgtcgt ggcgcggccg caatagccgg
     4081 agggcacgac ggccggacgg ccgtcgctca ggtaaccgat caggaatcgg acctaagccc
     4141 ttatcgcagt ttgctgttcg aggcaagatc atctcggatc gctcatcctg gttaacccgc
     4201 aacgcgacac gctccgcaac aattgttcca cgctcacgca agcgcgaggt agattgttgg
     4261 ccggattgtg aaagaggcgt ggtgtgcgta tcccggcgtt ccgcccagga tttctcgcac
     4321 tggttgtgtt ggccgaatgc gcgcgccccg cccccctcgc gacgaatccg tcccctgtgc
     4381 ggccgtctac tcccttcagc gccgccacca tcctgtcgat gcgcgccata gacacctcgg
     4441 cgggctccgc tccatggcgc gccgccttgc ttgccgccgc gagtgagggc agtggcggca
     4501 gcacttcccc cgctccgctc acggaattca tcgtgcgggc agacgacggc gccaccctgt
     4561 caatcgtgca atccaacaaa ctggacctgc ataccgggga gcgagtggtg atccgctatg
     4621 aaccgaccgt acaactcgcg cggcccggct gatcccaccc cttggccttt catccgggat
     4681 cggctatcgc ccccgcgcct atgaacttcg tcccaccacg accgacagca cgcatgaccg
     4741 tcctggaatt ccggggcgac atgtcagcgg caacacgcaa tcgcctggcc ttgctcgaca
     4801 tcgtcgaggg cattgccctc tggcggctcg cctggtcgct cggctggctc gacatccgcc
     4861 tgcgctaccg cggctccatc ctcggcccct tctggctaac tctttccact ggcgtgatga
     4921 tcggctcgct gggcgttctg tattccgcgc tgttccacat cgacacccgc aactatctgc
     4981 cgttcctagc tttgtcgcag gtgttgtggg gcttcattgc caccctcgtg ggcgaagcat
     5041 gtgccgcctt cacgcaggcg gaaggtatga tccgggccgt gcgcatgccg ctgttcgtgc
     5101 atgccattcg aaccttgctg cgcaacgtgt tcgtgctggc gcacaccatc gtcgtgatcg
     5161 tcgtggtcga cgtcatcttt tccgtatcgc cgggctggca tgcgcctctg gccttgccgg
     5221 gcgtcgtgct ctggatgttc gacggcctgg cgatctgctt gctgctcggc gcgttctgcg
     5281 cacggttccg cgacatcgcg ccaatcgtcg caagcatcat gcagatcgcg ttcttcctca
     5341 cccccgtgat ctggaagccc gaacagcttg gcggccatgc ctggctgctg ccgctcaatc
     5401 cgttcttcgc cattatggag atcgtgcgcg cgccactgct cggcacttct ccaagccata
     5461 caacgtggct tgccgcgatc gtctacagcg tcgtgctgtt cgcgttgtcc tggctgatgt
     5521 tcgtcaaggc acgtggccgc gtcgcattct ggatgtgaac atgagcagcg cccgtatcga
     5581 catcaccgat gtctcggtct tcttccccct gtatcacggc agcagccgca gcctgaagaa
     5641 gacggtcatg gcggccgcat ccggacgtct cggacaggat cgccagtcgc gcgtcgtcgt
     5701 gcaggcgctg cgcgatatct cgttcagcct gcaaagcggc gatcgtctcg gcctggtggg
     5761 cgcgaacgga gctggcaaga ccaccctgtt gcgcacgctg ggcggcatct atgagccggt
     5821 gaccggacaa gtccggctgc agggcagcgt cacgacattg ctcgacacaa cacttggcat
     5881 gaacctcgag ttgaccggac gcgagaacat ccagcttcgc ggtctgtata ccggcgtcgc
     5941 tcgaaccgac attccggcaa tggaagaaga cgtcgctcag ttcgccggcc tcggcgattt
     6001 catcgatctg ccggtgcgca cctattcatc gggtatggtg gtccgccttg ccttcgctat
     6061 ggcaacagcc ttgcgcccgc agattctgct gatggacgaa tggttcatcg tcggcgacgc
     6121 tgaattcatg gagaaggcgc gagatcgcct ggaaggcatg gtgcgtggcg cggagatcat
     6181 ggtgctctcc acgcatgcac tcgagatcat ccggagctgg tgcacgcgcg tgctctggat
     6241 cgaccagggc cgcattcgcg aggacgggtc gccggatgag gtgattgcag cctatctcgg
     6301 ccaccctctg cccgcacccg ctccagcccc cgcggcgacc tgaaggagct gccgctacat
     6361 ggcgatcctg ccgcttgccg acctgacagt cctcgaattc agccacacgg tgatgggtcc
     6421 gtcctgcggc gtcatgctcg ccgatctcgg cgccgacgtg atccgggtgg agccggcgcc
     6481 tgatggcgac cgcacccgcc ggctgcacgg cttcgccacg ggcttctttg cctacttcaa
     6541 ccgcaacaag cgctgtatct gcatcgacct gaaatcaccg gacggccagg ccgtcgcgca
     6601 cgacctgctg ggcaaggcgg acgtgctgat cgaaaatttc ggacccggca cgatggaccg
     6661 gctcggtctc ggctgggaca cggtgcatgc actgaacccg cgcctgatct attgcgcgct
     6721 gaagggcttc ctgcccggcc cctacgagca tcgtccgtcg ctcgacgaga tcgcgcagta
     6781 catgaccggc ctcgcctaca tgaccggacc gccaggccag ccactgcgcg ccggttcgtc
     6841 cgtgatcgac atcatgggcg gcgtcatggg tgcgatggcc atcctcgcgg cgctccgcca
     6901 gcgcgacaag gatggtatcg gccgcaaggt caccagcgcg ctttacgaaa gctccgcctt
     6961 cctggtgggc cagcacatgg cgggagaggc cgccacaggc acgccgtcgc cacccatgcc
     7021 cgcgcgcagc ggcgcctggg gcatctatga aaccttcccc accctgaacg gcgaaaagct
     7081 gttcatcggc atcacctccg ataaccactg gcgctccttc tgcgccatgt tcgaccggcc
     7141 cgatctgctt gcggatgaac gcttcaccac caatgccgat cgcgtgcgca atcgcccgcc
     7201 cctccgggac attgtcgcag ccatcgccag tcagcacgcg atcgccgatc tggaacggat
     7261 gatggatgca gcgaacattc cgttctcccc ggtgcgcacg ccatccgacc tgttcgacga
     7321 tccgcagctc aacgcattcg gccgcgcgct ggcagtgcgc atgcccaaag gcaacgtcgc
     7381 caacctgcca agcctgccat tctgcatcga cgacgaggca cccgccctgc gcatgcagcc
     7441 ccctgccgca ggcgaacact ccgacgctat cctcgccacg ctcggctatt ccgccgaaca
     7501 gattgccaaa ctgcgcgacg ccaggacgat cgcatagcat tgtcgttgcg aggagcgcag
     7561 cgacgaagca atctcgggcg aaattaggac aatatcgcca agcttgcctc atcgcattcg
     7621 caacgacact gagcggaaac agccgcccct tcctctcaac gttcgtcgct actcgttcag
     7681 aaaccgatcc accaaccgat ccgctcgctg ccgatccccg cgctggtgcg cgtgcatcag
     7741 gttgcgcgac cccgatccaa gatagaaccg ctcgtactgt tcgtttcggc tcgccaacgc
     7801 gctgccggca aaatcccacg cgagacggaa gatgcgcgcc cgctcctccg cgcccatctc
     7861 acctgcccca cccagcatct catccagcac cggccgcaac gccggattgg cgaactgcgc
     7921 ctctgtcggg gtcgtcagca gattgtgcga gcccaattcc cggatgatct cattcacccg
     7981 cggaaaccac cgaggcaggc tcgcgcgcag cgcgatcagc ggctccactg ccggaaacca
     8041 tacaccatcc ccatattcac gcgcatccac ctccgccgca cgcagcgatg accgggcgaa
     8101 ttgcgcaaac gtccagatct cgcccagcat ctgcaacgcc ggaggcagcg aagcgttgat
     8161 cgcctccgcc atccgggacg cgatccccca ggcaaacgtc agcttggtct ccgcgcggat
     8221 catcgcctgc tgcatgatgt tcggccacca actcgaccga agtatcccgt tgtacaccgc
     8281 caagttccca tcgatgaaaa cccgatcccg cgggacctcc acattgtcga agatcacgaa
     8341 tgcatcctgc tcatcgaacc ggctagagaa cggattgtcg aacaacgttc ccggcaccga
     8401 aacgctgtca cggcatagga acttcagccc cggtgtcgcc atcggaatgg aaaagctcaa
     8461 cgcgtgggtc ggattcgcat ccggcatcgg cgccgccgga tacaccgcca cgtcatcggc
     8521 aaacggcgcc aaggtggcca gcacgcgcgc gccacgcacg acgatcccgt gctccgtgtc
     8581 gcacaccttg tgcagcgaca cgttgtcgac ccccaaaggc accgctcccc gtgcccgatc
     8641 cgttgtggga tggatgatcg cgtgcgtcag cgacagatcg gcgcgcgcca tatgcttctg
     8701 gtagcggatc agattttccg caccccgctc gttgccgctg tgcgaccact cgccatgaca
     8761 gcctgcgaac cctgcatacg tgacgttcac gtagtcgggc gtccgcccca tcagcccgac
     8821 tgtatattca gcgatcgcct gtaaccctgc gtgccgccga accagatcct cgcgggagcg
     8881 cgggatcatg tggctgacgt tgatcggctc gccggtctcc ggatccggca taaggcacac
     8941 gtcggcacgt tcgtgctgca ggtcgaacac agcggcgatc gaatgcgccg cgcccttcag
     9001 cgcagggtgc gtcgcagcat cactgatccg ctccctaccg agccagacct cgcggtcatc
     9061 attcaatccc cgaagaaatt gttcccccga ccgagctgcc accgcctgat ccccctctcc
     9121 gcccccgggg gcggagaggc cgggtgaggt ggggaaccgc gccgccccac tcaccaactc
     9181 accgccacaa aagagggaat tgtgcggtct attccttcac cgcgccagtc atcgacgaca
     9241 cgtagtactc gacgaagaac gaatagatca gcgccactgg caccgatccc agcagcgaac
     9301 ccgccatcaa cgcaccccac tggtacacgt cgcccgtgac cagctcagtc aggatcgcca
     9361 ccggcactgt cttcttgtcc gtgctctgta tgaacgccag cgcatagatg aattcgttcc
     9421 agctcaacgt gaatgcgaag ataccagccg agatcaaccc cggaaccgcc aaaggcagcg
     9481 tgatccgccg caggatctgc agccgcgacg cgccgtcgat cagcgcgcat tcctccagct
     9541 catacggaat cgacttgaag tagccgatca gcagccaggt gcagaacggc accaggaagg
     9601 tcggataggt cagaaccagc gccaccggat tgtcgaacag tccgaattgg tagatcagcg
     9661 tcgccagcgg gatgaacagg atggatggcg gcaccagata tgcgaggtag atcgcaaggc
     9721 cgacatgctg cgcgcccttg aacttgagcc gttcgatcgc gtaggcggcc aggacgcttg
     9781 cgccaaccga caggaaggtc gcggcgacag caatgcccat ggtggtcatc agccaacgtg
     9841 gatagttggt ctgaaacagc agcacctcga tgttgtgcaa cgtcggcgag tgcacccaga
     9901 acggattgaa ccggtgatag tcatacatct cgtcgtccgg tttgatcgac gtgatggcca
     9961 tccagtagaa cgggaacagc agcacgatta cgaacagcgc cagcggaata tagatcgtga
    10021 cggtgcggcg cagctttgaa tcccacgcca gcgtctctgg ccgctcgtag ccgacggcgg
    10081 cctgtacttc ggccggcgat tccgggacca gcgcgtcagt cattcgcttc gccttgctgc
    10141 catttgcggc ggccgacacc gaagaagcac agcagcgtcg cgccgataag gaatgggatc
    10201 atcgacaccg caatcgctgc cccttcacca agctgcgccc cagggattgc ccgctggaac
    10261 gccagtgtcg ccatcaggtg cgtggtgttg aatgggccgc cgcgggtgat cgcgtaaacc
    10321 aactggaagt cggagaacgt gaacagcacc gacagcgtca acgccacagc cagtatcggc
    10381 atcagtagcg gcacggtgat gcggcggaat ttttgccagc cgctggcgcc atccagcagc
    10441 gccgcctcat acagcgaggg cgatatcgtc tgcagccctg ccagcagcgt aatcgccacg
    10501 aaggggatgc cacgccagat attggcgacg atcagcgacc agcgcgccgg ccagggcgtg
    10561 cccaggaagt tgatgtaggt gtcgcgcagg tgaagcacgt ccaccacgac gtaggagatg
    10621 atggagaact gcggcgagta gatccaccac caggcgattg ccgatagcac cgtgggcacg
    10681 atccatggca gcagcacgat ggcgcggatg aatgccttga acgggatgtg gtgattcagc
    10741 agcagcgcca gccacaggcc cagcgcgaat ttgccgatcg tagcgacagt tgtgtagaag
    10801 atcgtgttgg tgacagacat ccagaagatc ggatcgctca tcaggtcgac gaagttgtcg
    10861 aggccgatga actgaccggg ctggccaatg gtcgcgtcgg tcatcgacag ccagagccca
    10921 agaccgagtg gataggtcag gaacaccacc aacaggccga tcgtcggcag catgcacagg
    10981 aagacgagga acgacttgtt gtcgaacagc cgcgtcagcc agccatccgg cttgcgggtg
    11041 aaggtcagcc gctgcaggcc gacgtcggcg gttgttgtgc ccatcttggc ttcgtccctc
    11101 aacgggggcg ccaccacctg tggcagcgcc ccacgggtga tgtatcaggc cttcctgtag
    11161 taccgttcgg ctgcctttgc cgcctgcttc gcagctgact ccggcgtcgc gttgccggac
    11221 accaccgagg cgaacatgtc caccacggtg tagttggcga tcaccgccgc cgatgccgcg
    11281 ctgatcggtc ccttgtaacc gtcatagaac ggcgaatcca acgcggtggc gaacggcttc
    11341 agcttcggat ccgagttcca gaagttcatc ttgctgtagg ccttcagcgg ctggcaccag
    11401 tagccgaggc agctcgacag ccacaggcca tactggtcct tctccatcat gaagcggatg
    11461 tactccttcg ccgcgttggg atacttgctg tgcttgaaca gcatggcgct gatcggcgct
    11521 gccgacatcg gcttctgctt cgacaggcca tacggcgtgt cttgcgtgaa ggtgtccgcg
    11581 gccatcgcct gcaccttctg atcaggcgat ttcaacatcg aataatacag cgacacaccg
    11641 ttgaacgtca tgccgatggc gcctgactgg taggcctggt tgttgccgac atcgttccag
    11701 gtcagcgtgc cgggaatcat cgtcttctgc agctcggtcg cgtatttcag cgccgctatc
    11761 gtctccttgg agtcgagcgc cactttgcct gcctcgtcca ccacatacgc gttgtgcgac
    11821 cacagcagcc attcggaata gccgttggca tcgcccagcg cgtggccgag tgacatgccc
    11881 caaggatgtc ccgccttctg cattttctgg caaaggtcaa ggaacttctc gaggtcgttg
    11941 gggatctcgt tgtagcccgc ctcgctggcc caggacttgc gatacagcac cggaccggta
    12001 ccgccaccca gcggcagcgc gatccagttc ttggtcttcc acttctgacc gtacaacagc
    12061 gccagcggat accagccacc gtatttcgca ccaaggtaat cggcgaggtc ggtcatctcg
    12121 accagcttct cggaatagat atgcgagtcg gcaccgaagc tgacgaagat gtcggcaccg
    12181 gcaccggtgt tggccaccac cgcaagctgc ggacgcaggt cttcccagct gatgaagtcg
    12241 acctttactt cgacaccggt cttcttggtg aattcttccg tgttcttctt ccagtagact
    12301 tcgtccggat caatgaattt cgccgggcgg atcatgtgca gcgacgcacc cttttcgatc
    12361 ttgaattccg ggggcttcac atccgcggta gggacatcgt cgaacgctgc ccgcgcaccg
    12421 atcatcggga tcgacgccgc ccccagcgcc gccgcgccga gagccagtga attacgccgc
    12481 gaaatcgtca ttcgatagtt tcctccattg ccggtcaccc gccccaggac cctcgcgacc
    12541 ggatactgag gatccattcc gccgccatat tccgctggcc atgcgagagt gtctagccag
    12601 cgcgttgtcc ggtcgctgcg tcgaacagat gaaccagcgc cggatcaggc gcgatgcgta
    12661 tcgtctctcc aggttttgct gaaatgcgtt cgcggaatgc acaaaccacc gacgcaccgg
    12721 cgaactccgc caccacctgc gtctcggacc cggtgggctc gaccacatgc accttggcag
    12781 gaaagccacc ggggctcagc tggaagtgct cgggacgcac gccgtatacc gcaggccggc
    12841 cgtctgaact cttgccgcca ttcggcaacg gcagagcctc gccgccagca tggaatgcac
    12901 cgccttcgat cttgccctgc aggaaattca tcgccggcga gccgatgaag ccggcaacaa
    12961 acaggttcgc cggccgatca tacagctcga gcggtgcacc gatctgctcg acgttgcctg
    13021 agttcatcac cacgatcttg tcggccatgg tcatggcctc gatctggtcg tgcgtcacgt
    13081 agaccgttgt ggtctccagc cgctgatgca gctccttgat ctcggcgcgc atcgcaacac
    13141 gcagcttggc atccaggttg gagagcggct cgtcgaacag gaatacctgc gggtttcgta
    13201 cgatggcgcg gcccatcgcg acgcgctgcc gctgaccgcc ggaaagctgg cgcggatagc
    13261 ggttcaggta ttgtgtcagc ccaaggattt gtgcagcgcg gc
//
LOCUS       DATGEM010000203         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_857704, whole genome shotgun sequence.
ACCESSION   DATGEM010000203 DATGEM010000000
VERSION     DATGEM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857704"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /locus_tag="VKQ27_06520"
     CDS             complement(<1..1649)
                     /locus_tag="VKQ27_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLY88621.1"
                     /translation="MNELVKIFGQPAGTQSFDQIQIAIASPERIRSWSFGEIKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYRGIICEKCGVEVTLSKVRRDRM
                     GHIELASPVAHIWFLKSLPSRIGLLLDMTLKDLERVLYFENYVVVEPGLTPLKMHQLL
                     NEEEFVRAQDEYGEDSFTAKIGAEAMKDMLSQINLEEEVERLRVELRETTSEARRKKL
                     VKRLKLVEAFTESNSRPEWMILEVVPVIPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLIELRAPDIIVRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLQQCGLPKKMALELFKPFIYSRLDAKGLS
                     TTVKQAKKLVEKEKPEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILSPANGKPIIVPSQDIV
                     LGLYYLSLETPAYRDTPDQETIAFGTIGDIEHALFAKRITL"
     gene            complement(1724..>2666)
                     /locus_tag="VKQ27_06525"
     CDS             complement(1724..>2666)
                     /locus_tag="VKQ27_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HLY88622.1"
                     /translation="VEKLQRGDELPPGVMKMVKVFVAVKRKLQPGDKMAGRHGNKGVI
                     SRITPVEDMPYLADGQAVDIVLNPLGVPSRMNVGQILETHLGWASANLGRQIGEMLDS
                     FRRQTNTLDDLRGRLKDIYGDDSYNEDVADMNDKEIVELAENLRPGVPFASPVFDGAR
                     EADIVHMLEKAGLSSSGQVTLIDGRTGEAFDRQVTVGYIYMLKLHHLVDDKIHARSIG
                     PYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVSGRTKVYEA
                     IVRGDDTFEAGIPESFNVLVKELRSLGLNVELNQREM"
BASE COUNT          471 a          833 c          836 g          526 t
ORIGIN      
        1 tgcagggtga tccgtttggc gaacaaggca tgctcaatgt cgccgatggt gccgaacgcg
       61 atggtttcct gatccggcgt atcacggtac gcaggggttt ccagcgacag atagtacaag
      121 ccgagaacga tgtcctggct gggcacgatg atcggcttac cgttggccgg actgaggatg
      181 ttgttggtcg acatcatcag cacgcgcgcc tcgagctgcg cctcgagcga cagcggcacg
      241 tgcaccgcca tctggtcgcc gtcgaagtcg gcgttgaacg cggcacagac cagcggatgc
      301 agctggatcg ccttgccctc gatcagcacc ggctcgaacg cctggatacc gaggcggtgc
      361 agcgtcgggg ctcggttgag cagcaccgga tgctcgcgga tcacctcgtc gaggatgtcc
      421 caaacctccg gcttctcctt ttcgaccagc ttcttcgcct gcttcaccgt ggtcgacagg
      481 cccttggcgt cgagccgcga atagatgaac ggcttgaaca gctcgagcgc catcttcttc
      541 ggcaggccgc actgttgcag cttgagctcc ggaccgacca cgatcaccga gcggcccgag
      601 tagtcgacgc gcttgccgag caggttctga cggaaccggc cctgcttgcc cttgagcata
      661 tcggcgagcg acttcagcgg ccgcttgttg gcgcccgtga tgacgcggcc gcggcggccg
      721 ttgtcgaaca gcgcatcgac cgcttcctgc agcatgcgct tctcgttgcg cacgatgatg
      781 tcgggcgcgc gcagctcgat cagccgcttc agtcggttgt tgcggttgat gacgcggcgg
      841 tagagatcat tcagatccga ggtcgcgaac cggccgccat cgaggggcac cagcggacgc
      901 agctcgggcg gaatgaccgg caccacttcc agaatcatcc attccggacg ggaattggat
      961 tccgtgaagg cctcgaccag cttcagccgc ttgaccagct tcttgcggcg cgcctcggag
     1021 gtcgtctcgc gcagttcgac gcgcagccgc tcgacctctt cctcgaggtt gatctggctg
     1081 agcatgtcct tcatcgcttc cgcgccgatc ttggcggtga agctgtcctc gccatactcg
     1141 tcctgggcgc ggacgaactc ctcctcgttc agcagctggt gcatcttgag cggcgtcagg
     1201 ccgggctcga ccacgacgta gttctcgaaa taaagaacgc gctccagatc cttcagcgtc
     1261 atgtcgagca gcaggccgat gcggctcggc agcgacttca ggaaccagat gtgagcgacc
     1321 ggcgaggcca gctcgatatg gcccatgcgg tcgcggcgca ccttggacag cgtgacttcg
     1381 acgccgcact tctcgcagat gatgccgcga tacttcatgc gcttgtactt gccgcacagg
     1441 cactcgtaat ccttgatcgg cccgaagatg cgggcgcaga acaggccgtc gcgctcgggc
     1501 ttgaaggtgc ggtaattgat ggtttcgggc ttcttgatct cgccgaacga ccaggagcgg
     1561 atacgctcgg gactggcgat ggcgatctgg atctggtcga agctctgcgt tccggcgggc
     1621 tggccgaaaa ttttaaccag ttcgttcata aggatgctcc tgctgcacta gggcattaaa
     1681 gtcggcggcc aggacgggcc gtagcccgtc ccagccacct cgatcacatt tcgcgctggt
     1741 taagctcgac attgaggcca agcgaacgca gttccttgac cagaacgttg aacgactcgg
     1801 gaatgccggc ttcgaaggtg tcgtcgccgc gcacgatggc ctcgtagacc ttcgtacggc
     1861 cggagacgtc gtccgacttg accgtgagca tttcctgcag cgtataggcg gcgccgtaag
     1921 cttccagagc ccacacttcc atttcgccga agcgctggcc gccgaactgc gccttaccgc
     1981 ccagcggctg ctgggtgacg aggctgtagg ggccgatgga gcgggcgtgg atcttgtcgt
     2041 cgaccaggtg atggagcttc agcatataga tgtagcccac ggtcacttgg cgatcgaaag
     2101 cctcgccggt acggccgtcg atcagggtga cctgaccgga ggacgacagg cccgcctttt
     2161 ccagcatatg gacgatgtcc gcttcacgcg caccgtcgaa caccggcgaa gcgaaaggca
     2221 cgccaggacg gagattttcc gccagttcga cgatctcctt gtcgttcatg tcggcgacgt
     2281 cctcgttata ggagtcatcg ccatagatgt ccttcaggcg tccacgcaga tcgtccagcg
     2341 tgttggtctg gcggcggaag ctgtcgagca tctcgccgat ctgacggccg agattggctg
     2401 aagcccagcc gagatgggtc tcgaggatct gaccgacatt catgcgcgac ggcacgccca
     2461 gcggattgag gacgatgtcc accgcctggc cgtcggccag atagggcatg tcttcgaccg
     2521 gggtaatgcg cgagatgacg cccttattgc cgtgacggcc ggccatcttg tcgcccggct
     2581 gcagcttgcg cttcaccgcg acgaacacct tgaccatctt catcacgccg ggcggcagct
     2641 cgtcgccgcg ctgcagcttc tcgacc
//
LOCUS       DATGEM010000204         4761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_517607, whole genome shotgun sequence.
ACCESSION   DATGEM010000204 DATGEM010000000
VERSION     DATGEM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517607"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1064)
                     /locus_tag="VKQ27_06530"
     CDS             complement(48..1064)
                     /locus_tag="VKQ27_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HLY88623.1"
                     /translation="MRSLSRIGQLRPDAFVTALVGTIIVATLLPCQGISARLFHMAGI
                     AAIASLFFLQGARLSREAVIAGLTNYRLHTAIASTTFLLFPLLGFGLALLLPHALSPA
                     LLLGLLFLCALPSTVQSSIALTSIAQGNVAGAVCAATASSLVGILLTPLLFGFMSHVH
                     GHGAVDLSGMWLVILQLLVPFAIGHLMRPWIGPWAARNKRLLSVTDRGSILIVVYTAF
                     SAAVVHGIWHQLPPGMLATLGLVVAALLAMGLLVTTGVARAGGFSHADEVAAVICGSQ
                     KSLVSGIPIASVLFSGPTLGFIVLPIMLYHPLQLVVCAWLARRYATRPVAVVTAKELV
                     QAAV"
     gene            complement(1142..1432)
                     /locus_tag="VKQ27_06535"
     CDS             complement(1142..1432)
                     /locus_tag="VKQ27_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88624.1"
                     /translation="MRQILIASALAAGLIALWAAILPLTAQPAPHQTSVAPEALSKAL
                     FLCRGANGIDRGCAVALAGALAFDEQDQQVTRVSDQLCSANPRVLAKLVRRG"
     gene            complement(1531..1830)
                     /locus_tag="VKQ27_06540"
     CDS             complement(1531..1830)
                     /locus_tag="VKQ27_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88625.1"
                     /translation="MTSSVARNSFAKRLAAGCAVVGLAAIGLVAMPRQANAWWYNHGW
                     GYGVGVYVPPVVVAPAPAYYPAPAYYAPPAYYAPPARAWIPAHYQNGYYIPGHWS"
     gene            complement(2015..>4761)
                     /gene="gcvP"
                     /locus_tag="VKQ27_06545"
     CDS             complement(2015..>4761)
                     /gene="gcvP"
                     /locus_tag="VKQ27_06545"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HLY88626.1"
                     /translation="AKTVPPAIRTNQALDLPPPIDEAAVMAELRALAAKNGGAKSLIG
                     MGYHGTHTPPVIQRNVLENPGWYTAYTPYQAEIAQGRLEALLNFQTMICELTAMEIAN
                     ASLLDEGTAAAEAMAMAHAISKTKSDVLAVAADLHPQTRAVLATRAKPIGITLVDFAP
                     SDLAGIAAAQPFAVVLQYPGTTGAIRELSPEIGAAHEVDALVVVCADPLALALLTPPG
                     EMGADVVVGSSQRFGVPMGFGGPHAGYMATRDAYKRHMPGRLVGVSLDASGQPAMRLA
                     LQTREQHIRREKATSNICTSQVLLAVIAGCYAVWHGPQGLKRIARRVNLQARMLAGAV
                     RQGGHRLVHDAFFDTVAIETAGADTLMKAALAAGFNLRRISDTRVGIALDETVTREEL
                     MRLLAVVRGRDAPGQGVMGSIPAELVRNTPFLEQVVFHQHHAEHEMLRYLKRLEGKDI
                     ALNRSMIPLGSCTMKLNGSAEMLPITLPGFTDIHPFAPADQTEGYRELIARLTEWLQR
                     ITGFAALTLQPNAGSQGEYSGLLAIRAYHASRGEGQRSICLIPSSAHGTNPASAAMAG
                     LQVVVVGCDRDGNIDVADLRAKADKHASNLAALMVTYPSTHGVFEAAIREICEIVHQR
                     GGQVYMDGANLNAQVGLTSPAVIGADVCHLNLHKTFCIPHGGGGPGVGPIGVAAHLAP
                     FLPNHPLREDAGPATGFGPVSAGPFGSAMILPISYAYIRMMGAAGLKRATEVAILSAN
                     YIADRLRGHYPVLYTDPNGMVAHECIIDCRGFQAEAGVMVEDIAKRLMDFGFHAPTMS
                     WPVAGTLMIEPTESESKAELDRFCEAMIAIRAEIAAIAAGKLDKADNPLKHAPHTAAE
                     VMANVWTHGYSREQAAVPLPWVAASKYWPPVKRVDNVYGDRNLVCTCAPLEAYAQAAE
                     "
BASE COUNT          864 a         1683 c         1440 g          774 t
ORIGIN      
        1 cattgctccg gctttcgtac ttcgagaatg gtttcactcg ctacgagcta gacagccgcc
       61 tgcaccagct ccttggccgt taccacggcc accggccgcg tcgcgtaccg ccgtgccagc
      121 cacgcacaca ccacaagctg gagcgggtgg tacagcatga tcggcagcac gatgaaccca
      181 agcgtcggtc cagagaacaa aacgctggcg atcgggatac cgctcacgag cgacttctgt
      241 gagccgcaga tcaccgcggc cacctcatcg gcgtggctga acccgccggc ccgggcgaca
      301 ccggtcgtga ccagcaggcc cattgccagc agcgccgcca ccacaagccc aagcgtcgcc
      361 agcatgccgg gcggcaactg atgccagatc ccatgcacca ccgccgcgct gaacgccgtg
      421 tagaccacaa tcaggatcga gccgcggtca gtcaccgaca gcagccgctt gttccgcgcc
      481 gcccacgggc cgatccaagg gcgcatcaga tggcctatgg cgaagggcac cagaagctgc
      541 aggatcacca gccacatgcc ggatagatcg acggcaccgt gtccatgcac atgcgacatg
      601 aaaccgaaca gcagcggcgt cagcaggatg ccgacaaggc tggacgccgt cgcggcgcac
      661 accgctccag ccacgttccc ctgcgcgata gaggtcagcg caatcgagga ctgaactgtc
      721 gaaggcagcg cgcacaagaa tagcaagccc aacagcaggg cggggctcag tgcatgcggc
      781 agcagcaggg ccagcccgaa acccagcaac gggaacagca ggaacgtcgt gctcgcgatc
      841 gcggtgtgaa gccggtagtt tgtcaggccg gcgataacgg cctcgcgcga cagccgcgcg
      901 ccttgcagga aaaacagcga agcgatcgcc gcgatccccg ccatatgaaa cagccgggcg
      961 ctgattccct ggcagggcag cagcgtcgcg acgatgatcg ttccgaccag cgcggtcacg
     1021 aaggcgtccg ggcgcagttg tccgatccgg ctaagcgacc gcatgctggc ctcctgctct
     1081 ttactgggat gcaaccgtta cgcgctgcgc gtcatctccg cagtgaactg cgacacatcg
     1141 ctcaaccgcg gcggacgagc ttggcgagaa cgcgcgggtt ggcagagcaa agctggtcgc
     1201 tgacccgcgt cacctgctga tcctgttcat cgaacgccag cgcgcctgcc agcgccacgg
     1261 cgcagcctcg gtcgatgccg ttggcgccgc ggcagaggaa cagggccttc gacagcgctt
     1321 caggcgccac cgacgtctgg tgtggtgccg gctgggcagt caacggcagg atcgccgccc
     1381 agagcgcgat cagtccggct gccagagcgc tcgctatcag aatctgtcgc atggcaccct
     1441 ccatgggctt gtcaggtccc gcggtgcggg acggcgtgcg ggccacatag cggtgttccg
     1501 aacatggccc gtatgaaccg cttcacagga tcaggaccaa tggcccggaa tgtagtaacc
     1561 attctgataa tgcgccggga tccaggcacg ggccggtggt gcataatagg ccggcggtgc
     1621 gtagtaggcg ggagccgggt aataggccgg agcgggcgcc acgacgacgg gcggcacata
     1681 cacgccgacg ccgtagcccc agccatggtt gtaccaccag gcattcgcct gccgcggcat
     1741 cgcgacgagg ccgattgccg ccaagccaac caccgcgcag ccggcagcga gacgcttcgc
     1801 gaaggaattg cgtgcgaccg aggaggtcat gtttctctcc accaggtatt tcgttcggcg
     1861 aggcgactgc cttttgccgt gactgcaccg taggcgcggc cgttcatcgg gcgatatagg
     1921 agagagagtt cgcccgtctt ggctgaaaat gctgaaacga acctgagaaa aacccccacc
     1981 atttttccct cccccttccg ggaggagggg ggcgttattc cgccgcctgg gcgtaggcct
     2041 ccagcggcgc gcacgtgcaa acgaggttgc gatccccgta gacattgtcc acgcgcttta
     2101 ccggcggcca atacttgctc gccgccaccc agggcagcgg caccgccgcc tgttccctgg
     2161 agtaaccatg cgtccataca ttcgccatga cctcggcggc agtatgcggc gcgtgcttca
     2221 gcggattgtc cgccttatcc agcttgcctg ccgcgatcgc ggcaatctcc gcgcggatcg
     2281 cgatcattgc ctcgcagaac ctgtcgagct ccgccttgct ctcgctctcg gtcggctcga
     2341 tcatcagcgt gcccgccacc ggccatgaca ttgtcggcgc atggaagccg aaatccatca
     2401 gccgcttggc gatgtcttcc accatcacgc ccgcctcggc ctggaagcca cggcagtcga
     2461 tgatgcactc atgcgccacc atcccattcg ggtcggtgta gagcaccgga taatgcccgc
     2521 gcagccggtc ggcgatgtag ttggcgctca ggatcgccac ctcggtcgcg cgcttcagcc
     2581 ccgcagcccc catcatccgt atatacgcat atgagatcgg caggatcatc gcgctgccga
     2641 acgggcctgc cgacacaggc ccgaaccctg tcgccggccc tgcatcctcg cgcagcggat
     2701 gattcggcag gaacggtgcc agatgcgccg ccacgccaat cggtccaacg ccaggcccac
     2761 cgccgccatg cggaatacag aacgtcttgt gcaggttcag atggcagacg tcagccccga
     2821 tcacagccgg gctggtcagc ccgacttgcg cgttcagatt ggcgccatcc atgtaaacct
     2881 gcccgccgcg ctggtgcacg atctcgcaga tctcacggat cgccgcttcg aacacaccgt
     2941 gcgtgctggg atacgtcacc atcagcgccg ccaggttgga cgcgtgcttg tccgccttcg
     3001 cgcgcagatc ggccacatcg atgttgccgt cgcggtcgca gcccaccacc acgacctgca
     3061 gccccgccat cgcggcactc gccggatttg tcccgtgtgc gctcgatggg atcaggcaaa
     3121 tgctgcgctg cccctccccg cgcgatgcgt gatatgcacg gatcgccagc agcccggaat
     3181 actcgccttg cgaccccgca ttcggctgca acgtcagggc ggcaaagccg gtgatgcgct
     3241 gcaaccattc ggtcagccgc gcgatcagct cgcgataccc ctcagtctga tcggctggcg
     3301 cgaacggatg gatatcggtg aagcccggca gcgtgatcgg aagcatctcg gccgagccat
     3361 tgagcttcat tgtgcatgac cccaacggga tcatgctgcg gttcagcgcg atgtccttcc
     3421 cctcaaggcg cttgagatac cgcagcatct cgtgctcggc atgatgctgg tgaaagacca
     3481 cctgctccag gaaaggcgtg ttgcggacca gttcggcggg gatcgatccc atcacgccct
     3541 ggcctggggc gtcgcggccc cgcacaaccg caagcagccg catcaactct tcccgcgtca
     3601 ctgtctcgtc cagcgcaatc ccgacgcgcg tatcgctgat gcggcgcagg ttgaaccctg
     3661 ccgccagcgc tgccttcatc agcgtatcgg cacccgcggt ctcgatcgcg accgtgtcga
     3721 agaacgcgtc gtgcaccagt cgatggccgc cctgccgcac cgcgcccgcc agcatccgtg
     3781 cctgcaaatt cacccgccgg gcgattcgct tcagcccctg cggcccgtgc cacaccgcgt
     3841 agcatccggc gatcaccgcc agcagcacct gcgatgtgca gatgttcgac gtcgccttct
     3901 cgcgccggat atgctgttcg cgggtctgca acgcgagccg catggccggc tgccccgacg
     3961 catccagcga cacgcctacc agccgcccgg gcatgtgccg cttgtacgca tcacgcgtgg
     4021 ccatgtaccc ggcgtgcggc ccaccgaacc ccatcggcac accgaaccgc tgcgacgaac
     4081 ccaccacaac atcggcgccc atctcgcctg gcggcgtcag caatgccaac gccagcggat
     4141 cggcgcagac aaccaccagc gcatccacct catgcgccgc gccgatctcc ggcgacagtt
     4201 cccgtatggc acccgtcgtc cccggatatt gcagcaccac tgcaaacggc tgcgctgcgg
     4261 cgatccccgc caggtcgctt ggcgcgaaat ccaccagcgt gatgccgatg ggcttcgccc
     4321 gcgtcgcgag caccgctcgc gtctgcggat gcaggtccgc ggcaaccgcc agcacgtcgg
     4381 acttggtctt gctgatggca tgcgccatcg ccatcgcctc ggccgccgcg gtgccttcat
     4441 ccagaagcga ggcgttggcg atctccatcg ccgtcagctc gcagatcatc gtctggaagt
     4501 tgagcagcgc ctccagcctg ccttgcgcga tctccgcctg gtacggcgta taagcggtgt
     4561 accatccagg attttccagc acattccgct ggatcaccgg cggcgtatgc gtgccgtgat
     4621 aacccatgcc gatcagcgac ttcgccccgc cgttcttcgc ggccagtgcg cgcagctccg
     4681 ccatcaccgc cgcctcatcg atcggaggtg gcaggtccag cgcctggttg gtgcggatcg
     4741 ccggcggcac ggtcttcgcc g
//
LOCUS       DATGEM010000205         8615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_753702, whole genome shotgun sequence.
ACCESSION   DATGEM010000205 DATGEM010000000
VERSION     DATGEM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753702"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VKQ27_06550"
     CDS             complement(<1..1353)
                     /locus_tag="VKQ27_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLY88627.1"
                     /translation="MLFFRVSSRYHLAILWLVAIVTIGLLTGICFALSVPASTTSFIY
                     LMAIVLLSLWDSFVSSALFSVVAVVALDYFFIAPIFSLTIAAQSDIALFAAFLLTSFV
                     ITSLVRKLQASALMQTRQARLLDLTHDTVLVRDPRDVIVFWNRGAEQLYGWSRDEAVG
                     TVTHNLLHTIFPIPLETIKEQLRRAGHWEGELVHVRKDGSQVTVASRWSLQRDDQGQP
                     IGTLETNNDITERKRAEEALRRSQAAYLAEAQKLSQTGSFGWNTAGGEVFWSDQSFDI
                     LGYDQTVTPTLDLLIDRVHPDDMALVRATFEVAARDRSGFDIEFRLAMPGGVTKHIHA
                     VAHVMEDAPVTTQFVGALMDVTKIRQAEAQLHQAEAQVAHVARVTSLGALSASIAHEV
                     NQPLAAIVSHGEASLRWLNRDPPRLDEVEASVRHIVANGKRASEVIQRIRALTSGTVR
                     Q"
     gene            1574..6673
                     /locus_tag="VKQ27_06555"
     CDS             1574..6673
                     /locus_tag="VKQ27_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HLY88628.1"
                     /translation="MRRFGWLFAALLSLACGTAWADTFSLPGLESDANAYESTLTSHF
                     PAGGTPAARKQAEAQAAAAVKKQDWVAAAAAWEKRIGQGDAKPEQWLGLGEAQMHRTP
                     AEPKRALQAAWENYSALDTGEKQIPALMLMADALKALDRPAQAIQALEAAAELTTDNK
                     AIVAKLDEMRRATGILVRHVTTEAEADPPRACIDFTVAPARRDDFHAEDWVKLTPPLP
                     GAAVTREGDQICVSGLPSGATTGITFRAGMPGEGGLTLVKDTSLNVAMANRQPRIDFD
                     TRMFLLPRGQTPAIGLSTVNLSSVKLTLSRLTERNIIGWVREQKLGQPVDASDADNIG
                     DQSGRIIWEGKADIPGWVANRSAHTALPMPDALANAGPGLYALIVRAGDGTPTGASAV
                     QMILRTDFAPTVWRGTDGLTIQVRGYSDVLPRQGATLRLLAENNEILGETTTDADGIG
                     RFAAPLLHGEGATGPRAVEVMGGEDYTIIDLSSAAFDLSDRGVTGLPHPGPLDAYVWM
                     DRGIYRPGETVHVMTIVRDDAGKPADIPVHVIIKRPNGQVYLDATPARAAEASIHLPV
                     ALSSGAPAGNWSIEVKADPGLPPIGTGAFRVDAFVPDRMAVDLGPAAGPIVPGKPYSL
                     PITSRFLYGAPAAGLTGQGQMRLLIDPAPFPVLAGYLIGLVNETYAPDATDLQVPDTD
                     EHGHSSVAIDVPRAPDTTHALKASITVGVNDPSGHASLATTEIPVRPAGNLIGIKPAF
                     ADFAVDAGTEAGFDIAAVDPSGTRIALKAKLRLVRERPDWRLVMHGNLARYETVWRDQ
                     PLETTAVDIPADKPLHYAKTLDFGRYRLEVLENNGMAATSVRFRSGWVSSDNPDVPDQ
                     VDVSADKKAYTPGDTAHIHIAPPFAGQATLLVLTDRVHLTRDLPVAAGGTDVDVPVSA
                     DWGPGAYVTVHVFRTAADAKSRPSRAIGLAWVGIDPGVRKIPVSFDVPDKYPPRARAT
                     IKVHAAPGAWVSLAAVDEGILRLTRFVSPDPTNHFLGRRKLGMDIRDDWGRLIAPPDG
                     TATVLRQGGDEGSFVLPEIPQKTVTLFDAPVQAGPDGVAELPLDFPDFNGQVRLMAVA
                     WSGTHLGAASTDVYVRDPLVAEPLLPRFLAPGDQARLTLLLQNLELPAGEQAVTFSVD
                     GPLEITGEKRLAATLGPNAQALPFTTLTATGAGRGVVRLDVQGTGGFHIQRETAIWVR
                     PARGPTTIIAAGDLAPRAEMQLAPPLSQFIAGTWKASATFGGVVHYDVAGLVQDLDDY
                     PLWCLEQATSRGFPLAVLQDGPVAGPDRAARLQQAVGFVLDRQRFDGGFGLWSASEEA
                     EPWLSVYATDFLLRARQAGAAVPDQALIDAKKFIAGAADEPGDKPDDMAAQAYRLYVL
                     ALAGQGRPGAARVMAERINTLPTPLAKAQIGAALALAHDEPRAQAAFAAALAAPARKW
                     WSFDYGTALRDQAAIAVLLKESGLPGDQLKKLVDAMPGADLSPETLSTQEQAWAATAG
                     AVLSRGAAPAHVALDGKDLPEGPVVSVALSGPANVRNLSSQPVWRAVSATGVSASPLP
                     AARSGMRITRQFKTLDGQTLDLDHLKQNQAFVIVLDGKAEDGQPHRAMILHGLPAGWE
                     VAGKLGSGDAPGMSWLGKLSDTEAQPAADDRYAAVVALTPEHPDFHLAVRIRAVTPGT
                     FELPGAEVADMYRPGVFARQAAGRITIVGAE"
     gene            6670..>8615
                     /gene="pbpC"
                     /locus_tag="VKQ27_06560"
     CDS             6670..>8615
                     /gene="pbpC"
                     /locus_tag="VKQ27_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HLY88629.1"
                     /translation="MSKDGRIWGFCRRALATLPSLLFVLSGVPIALDRIFPPDLSRLA
                     SVGTEVVDRNDRPLAVLPASGGVWRFRADTEPPLVTNLLIAVEDKRFWYHPGVDPLAL
                     IRATAQLVRSGRIISGGSTIAMQAARLLHPRARTVPAKLIEMARALQLELRFGRRGVL
                     DIWLTLAPFGGNLEGIRAGSLAWFGVPPEALEPTQAALLVAIPRRPERLRPDRHPDAA
                     RIVRDRVLAVGYRAGLFDPSGGPVPAARLPLPRHAPQLVASLPREPLVHTTLDLPLQA
                     ALERLASDSIQSLAKRASLAILIADASSREILALYPGAWRDQDRAGSLDLTKAVRSPG
                     SALKPFIYAMAFSDGVAAPDTIVPDLPRRFGGYAPENFDRGFAGSITAGEALQRSLNL
                     PAVALLDRVGPLRFAATLRAAGVTLRLPRGADPSLPLALGGDGITLRQAAALYAALAT
                     DGTGGPLRLLTDARPSPRPAFLSAAAAREVADVLTRPLPDFGPRGIAWKTGTSWGGRD
                     AWALGFDRRHVVAVWIGRPDGTPLPGATGASLALPELSHVFDLLPKLPRSTSHIHERL
                     RQPSVVRADALHLLFPPAGSVLSADGPVTIRVMGGRRPLTFMVDGAPLPTDRIRRDVA
                     WQPAGPGFYHLTVLDADGTAVRANV"
BASE COUNT         1379 a         3012 c         2759 g         1465 t
ORIGIN      
        1 ctgccgcacc gttccgctgg tcagggcgcg gatgcgctgg atgacctcgc tggcacgctt
       61 gccgttggcg acgatgtgcc ggaccgaggc ctcgacctcg tccagccgcg gcggatcgcg
      121 attgagccag cgcaggcttg cctcgccatg cgatacgatc gcggcgagcg gctggttcac
      181 ctcatgcgca atcgaggcgg acagtgcgcc aaggctggtg acccgcgcca cgtgcgcgac
      241 ctgcgcctcg gcctgatgaa gctgggcctc agcctgcctg atctttgtca cgtccatcag
      301 cgcgccgacg aactgcgttg tgaccggcgc atcctccatg acatgagcca ccgcgtggat
      361 atgcttcgtc acaccgccgg gcatcgcgag ccgaaattcg atgtcgaagc cgctgcggtc
      421 gcgcgctgcc acttcaaatg tcgcgcgcac caaggccata tcatcggggt gaacccggtc
      481 aatcagcaga tcgagggttg gcgtcaccgt ctgatcgtac cccaggatgt caaagctctg
      541 atctgaccag aacacctcac cacctgccgt attccagccg aaactgcccg tctggctcag
      601 cttctgcgct tcggcgagat aggccgcctg gctgcggcgc agcgcttcct cggcacgctt
      661 gcgttcggtg atgtcgttgt tggtttccag cgtgccgatt ggctgcccct ggtcgtcacg
      721 ctgcaacgac caccgactcg cgacggtcac ctgtgagccg tccttgcgta catggacgag
      781 ctctccctcc caatggcccg cccgccgcaa ctgttccttg atcgtctcca gcggaatggg
      841 aaagatcgta tgcaacaggt tgtgtgtgac cgtccccact gcctcatcgc ggctccagcc
      901 gtaaagctgc tcagcgcccc ggttccagaa gacgatgaca tcgcgcggat cacgcacgag
      961 caccgtgtca tgcgtcaggt cgagcaggcg cgcctgcctc gtctgcatca gcgccgaagc
     1021 ctgcagcttg cgcacgaggc tggtgatcac gaaggaggta agcagaaacg ccgcaaataa
     1081 cgcgatgtcg ctctgcgccg cgatggtcaa ggaaaatatt ggagcgatga agaagtagtc
     1141 gagagcaaca acggcaacga ctgaaaagag cgccgacgac acgaaactgt cccaaagcga
     1201 cagtaggacg atcgccatca ggtagatgaa actggtcgtg gacgcaggaa cgctcagggc
     1261 aaagcagatg cccgtcagca gcccgatcgt gacgatggca accagccaca atatcgccag
     1321 gtgatatctg gatgagacgc ggaagaagag catgacgcga ccgaccgcag ctccggataa
     1381 gacgagatgc cgcgctaccc gcacgatggg cagcgcgatt ccattccggc tttatgcgat
     1441 ctgatgcctc ctgaggcaac gccggacatg caaaggtact cactgccgag cggcggattt
     1501 gcaggggagc atttttccca ccccagttgg gccccgtcgc attacactgc gccaatcgta
     1561 acggaggggc gtcatgcggc gtttcgggtg gctgttcgcg gcattgctgt cgctcgcttg
     1621 tggcacggct tgggccgaca ccttcagcct gcccgggcta gaatcggacg ccaacgccta
     1681 cgaaagcacc ctgacgtcgc acttcccggc cggcggcacg ccagccgcgc gcaagcaggc
     1741 cgaggcgcag gcggcggcgg ccgtcaagaa acaggactgg gtggccgccg cggcggcgtg
     1801 ggaaaagcgc atcggccagg gtgacgcgaa gcccgaacaa tggcttggac tgggcgaagc
     1861 gcagatgcat cgcacgcccg ccgaaccaaa gcgcgccctg caggccgcct gggagaatta
     1921 ctccgcgctc gacaccggcg aaaaacagat tcccgccctg atgctgatgg cggacgcact
     1981 gaaggcgctg gaccggcctg cacaagcgat ccaggcactg gaggcggcgg ccgaactcac
     2041 taccgacaac aaggccatcg tcgccaagct ggacgaaatg cggcgtgcca ccggcatcct
     2101 ggtccgccac gtcaccaccg aggcggaagc cgatcccccg cgcgcctgca tcgacttcac
     2161 ggtcgctccc gcccgccgtg acgattttca cgccgaggac tgggtgaagc tcacgccgcc
     2221 tctgcccggc gcggccgtca cccgcgaagg cgaccagatt tgcgtttccg ggctgccgtc
     2281 cggcgccacc acgggtatca cctttcgtgc cggtatgccc ggcgaggggg gcctcaccct
     2341 ggtgaaggac accagcctga acgtcgccat ggccaatcgc cagccgcgca tagactttga
     2401 cactcgcatg ttcctgctgc cgcgcggtca gaccccggcg atcggcctca gcactgtcaa
     2461 cctgtccagc gtcaagctga ccctgtcgcg gctgaccgag cgcaacatca tcggctgggt
     2521 ccgcgagcag aagctcggcc agcccgtgga tgcctcggat gccgataaca ttggcgacca
     2581 gagcggccgc atcatctggg agggcaaggc cgacataccg ggctgggtcg ccaaccgctc
     2641 ggcgcacacc gcgctgccga tgccagatgc gctggccaat gccgggcccg ggctttacgc
     2701 cctcatcgtg cgcgccggtg acggtacacc gacaggcgct tcggcggtgc agatgatcct
     2761 gcgcaccgat ttcgctccga ccgtctggcg cggtaccgat gggctgacaa tacaggtccg
     2821 cggctactcc gacgtcctgc cgcgtcaggg tgcgacgctg cgcctgctgg ccgagaacaa
     2881 cgaaatcctc ggcgagacca cgaccgatgc cgacggtatc ggccgcttcg cggccccgct
     2941 gctgcatggc gaaggcgcca ctggaccgcg cgcggtcgaa gtgatggggg gcgaagacta
     3001 caccatcatc gacctcagca gcgccgcctt cgatctctcc gatcgcggcg tgaccggact
     3061 gccgcatccg gggccgctcg atgcctatgt ctggatggat cgcgggatct atcgcccggg
     3121 cgaaaccgtc catgtcatga caatcgtccg cgacgatgcc ggcaagccgg ccgacattcc
     3181 ggtgcatgtc atcatcaaac ggcccaacgg tcaggtatat ctggatgcca cgccggcgcg
     3241 tgccgccgaa gcttcgatcc atctgcccgt tgcgctgtcg tcaggcgcgc cggcgggcaa
     3301 ctggtcgatc gaggtgaagg ctgatcccgg cctgcctccc atcggcactg gcgcgttccg
     3361 ggtcgacgca ttcgtgccag atcgcatggc tgtggatctc ggcccggccg ctggtccgat
     3421 cgtgccaggc aagccctatt ccctgccgat cacctcgcgc ttcctgtatg gcgcgcctgc
     3481 ggcgggtctg actggtcagg gccagatgcg gttgctgatc gatcccgcgc cgttccccgt
     3541 tctggctggc tatctcatcg ggctggtcaa cgagacctat gcgcccgatg ccaccgacct
     3601 gcaggtgccc gacactgatg agcacggcca cagctctgtc gccattgatg tgccgcgtgc
     3661 gcccgacacg actcacgcgc tgaaggcgtc gatcaccgtg ggcgtaaacg atccatccgg
     3721 ccacgcctcg ctcgccacca ccgagatacc cgtacgtcct gcgggcaacc tcatcggcat
     3781 caagcctgct tttgccgact tcgctgtgga tgccggcacc gaggccggtt tcgacatcgc
     3841 cgcagtcgat cccagcggaa cgcggatcgc gttgaaagcc aaattgcgcc tcgtgcgcga
     3901 acgtcctgac tggcgcctag tcatgcacgg caacctcgca cggtacgaga ccgtctggcg
     3961 cgaccaaccg ctggaaacga cggcggtcga catcccggcc gacaaaccgc ttcactatgc
     4021 caagacgctc gacttcggcc gctatcgcct ggaggtgctg gagaacaacg gcatggccgc
     4081 gacctccgtg cggttccgtt ccggctgggt gtcgtccgac aatccggatg tgccggacca
     4141 ggtggatgtc tcggccgaca agaaagccta tacgcccggc gatacggcgc acatccacat
     4201 cgcgccgccg tttgccggcc aggcgacgct gctggtgctg accgaccggg tgcacctgac
     4261 acgcgatctg cccgtggcgg caggtggcac tgacgtcgat gtgccggtgt ccgccgattg
     4321 ggggccaggc gcctatgtca cggtccacgt cttccgcacc gccgccgacg ccaaatcgcg
     4381 tcccagccgc gccattggcc tcgcctgggt cggcatcgat ccgggggtcc gcaagatccc
     4441 ggtcagcttc gacgtgccgg acaaatatcc gccgcgcgcc cgcgccacga tcaaggtgca
     4501 tgcggcgccg ggtgcctggg tcagcctcgc cgccgtggat gagggcatcc tgcgcctgac
     4561 acgtttcgtt tcgcccgatc ccaccaacca ttttcttggg cgccgcaagc tgggaatgga
     4621 tatccgcgac gactgggggc gtctgatcgc tccgcccgac ggcactgcga cggtgctgcg
     4681 acagggcggc gacgaaggca gcttcgtgct gccggaaatc ccgcagaaga cagtaacgct
     4741 gttcgatgca ccggtgcagg ccggccctga tggcgttgcc gaactgccgc tcgacttccc
     4801 tgacttcaac ggccaggtgc gattgatggc cgtggcctgg agcggtaccc atctcggtgc
     4861 tgcatccaca gatgtatatg tgcgtgatcc actggtggct gaaccgctgt tgccgcggtt
     4921 cctcgctccc ggcgatcagg cgcggctgac attgctgctg cagaatctgg agctgccggc
     4981 gggcgagcag gccgtcacct tctcggtgga tggccccctg gagatcaccg gcgagaagcg
     5041 tctcgccgcg acacttggcc ccaatgctca ggcgctgccg ttcaccacac tgaccgcgac
     5101 cggcgccggt cgcggcgtcg tacgtctgga cgtgcagggc accggcggct tccacatcca
     5161 gcgcgagaca gcgatctggg tgcgcccggc gcgcgggcct acgaccatca tagcggctgg
     5221 cgacctcgca ccgcgagcgg agatgcagct tgcaccgccg ctcagccagt tcatcgcggg
     5281 tacctggaag gcgtcggcca cctttggtgg tgtggtgcac tacgacgtag ccgggctggt
     5341 gcaagacctg gacgactatc cgctttggtg cctggagcag gcaacgagcc gaggcttccc
     5401 gctcgcggta ctgcaggatg gtccggtcgc cggtcccgat cgagcggcgc ggctgcaaca
     5461 ggcggtgggc ttcgtgctcg accgccagcg cttcgacggc ggcttcggcc tgtggtcggc
     5521 ttccgaggaa gcagaaccct ggctctcggt ttacgccacc gatttcctgc tccgcgcccg
     5581 ccaggccggg gccgcggtcc ccgatcaggc actgatcgat gcgaagaagt tcatcgccgg
     5641 tgccgccgat gaaccaggcg acaagccgga cgacatggcc gcccaggcct atcggctgta
     5701 cgtgctggca ctcgctggcc agggcaggcc tggtgcagct cgcgtgatgg ctgagcgtat
     5761 caacacgctg cccacaccgt tggcgaaggc gcagattggc gccgcgctgg cactggcgca
     5821 tgacgaaccg cgcgcccagg cggcgttcgc cgcggcgctt gccgcgcccg cacgcaagtg
     5881 gtggtccttc gattacggca ctgcgttgcg tgaccaggct gcgattgccg tcctgctgaa
     5941 ggagagcggg ctacccggcg atcaactgaa gaagctggtc gatgccatgc caggagccga
     6001 cctctcaccc gagaccctgt ccacgcaaga gcaagcgtgg gcggcgacag ccggtgccgt
     6061 actgagccga ggtgctgccc cggcacacgt cgccctcgat ggcaaggact tgccagaggg
     6121 gccggtcgtg tccgtggccc tcagcggtcc ggccaacgtc cgcaacctgt cgagccagcc
     6181 ggtctggcgg gccgtgtcgg ccacaggggt ctcagcctcg ccactccctg ccgcacgctc
     6241 cggcatgcgc atcacgcggc agttcaagac gctggatggc cagacgctgg acctcgatca
     6301 cctgaaacag aatcaggcct tcgtcatcgt gctcgacggc aaggccgagg acggccagcc
     6361 gcatcgcgcc atgatcctgc atggcctgcc cgcgggctgg gaggttgccg gcaagcttgg
     6421 cagcggcgat gcgccgggaa tgtcctggct cggcaagctg tcggacaccg aggcgcagcc
     6481 cgcggccgat gaccgctatg cggccgtcgt ggcgctcaca ccggagcatc cggatttcca
     6541 tctggccgtc cggatccgcg cagtgacgcc gggcacgttc gagctgccgg gtgccgaggt
     6601 ggctgacatg taccgccctg gcgtctttgc tcgtcaggcg gccggccgga tcacgattgt
     6661 gggcgccgag tgagcaagga cgggaggatc tggggatttt gccgccgcgc tctggcaact
     6721 ctgccctccc tgctgttcgt cctttcgggt gtgccgatcg ccctcgatcg catcttcccg
     6781 cccgacctct cccggctcgc ctccgtcggc accgaggtag tggaccgtaa cgaccggccg
     6841 ctcgcggtgc ttccagcctc tgggggcgtc tggcggttcc gtgcggatac cgaaccgccc
     6901 ctcgtcacca acctgctgat agcagtcgag gataagcgct tttggtacca ccccggcgtc
     6961 gatccgctgg ccctgatccg cgccacggcg caactcgtcc gctcaggtcg catcatctcc
     7021 ggcggctcca cgatcgccat gcaggccgcc cgcctgctac atccccgcgc acgcacagtc
     7081 cccgcaaagc tgatcgagat ggcgcgcgcg cttcagctcg aactgcgctt cggccgccgc
     7141 ggcgtcctcg atatctggct gaccttggcg ccgttcggcg gcaacctgga gggcattcgc
     7201 gccggctcgc tggcctggtt cggtgtaccg cccgaggcat tggagcccac tcaagccgca
     7261 ttgctcgtcg ccattcctcg ccgcccggag cgcctgcgcc ccgaccgtca ccccgatgca
     7321 gcccgtatcg tgcgcgatcg ggtgctggcc gtcggttatc gcgccggtct gttcgatccg
     7381 tccggtggcc cggtacctgc cgcgcgtctg ccactgcctc gccatgcccc gcaacttgtc
     7441 gcttcgctgc cccgcgagcc gctggtccac acgaccctcg atctgccact tcaggcagcg
     7501 ctggagcggc ttgccagcga cagcatccaa tcgctggcga agcgcgcctc gcttgccatc
     7561 ctgatcgccg atgcctccag tcgggagatc cttgcgctgt atcccggcgc ctggcgtgac
     7621 caggatcgcg ccggctcgct cgacctcacc aaggccgtcc gctcccccgg ttcagctctg
     7681 aagccgttca tctatgcgat ggccttctcc gacggtgtcg ctgcgccgga caccatagtc
     7741 cccgacctgc cgcgccgttt cggaggctat gcgcccgaga acttcgaccg aggcttcgcc
     7801 ggcagcatca ccgccggcga ggcgctgcag cgctccctca acctgccggc tgtggccctg
     7861 ctcgaccggg ttggcccgct gcgctttgcc gcgacattgc gggctgccgg cgtgacgctg
     7921 cggttgcctc gcggtgcaga tccgtcgctg ccgctggcgc tcggcggcga cggcatcacc
     7981 cttcgccagg cagcggcgct ctatgcggcg ctggccaccg acggcaccgg cggcccactt
     8041 cgcctgctga ccgacgcgcg cccttcgccg cggcccgcgt ttctctcggc cgctgcggca
     8101 cgcgaggttg cagacgtgct cacacgcccg ctgccggact tcggcccgcg cggcattgcc
     8161 tggaagactg gcaccagttg gggcgggcgc gatgcctggg cgcttggctt cgacaggcgg
     8221 catgtcgttg cggtatggat cggacgtccg gatggcacgc cgttaccggg ggccactggc
     8281 gcatcgctag cactgccaga gctgtcccac gtgttcgatt tgctgccgaa actgcctcgc
     8341 tccacctcgc atatccatga gcggctcagg cagccatccg tcgtgcgcgc cgatgcgcta
     8401 catctgctgt tcccgcccgc gggctccgtt ctgtccgccg atgggccggt caccatccgc
     8461 gtgatgggcg ggcgccggcc attgaccttc atggtcgacg gggcaccgct tcccactgac
     8521 cgcatccggc gggatgtggc ctggcagccg gccggtcccg gcttctatca tctgaccgtg
     8581 ctcgacgccg acggcaccgc ggtgcgcgcc aacgt
//
LOCUS       DATGEM010000206         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1313237, whole genome shotgun sequence.
ACCESSION   DATGEM010000206 DATGEM010000000
VERSION     DATGEM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313237"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="VKQ27_06565"
     CDS             <1..1715
                     /locus_tag="VKQ27_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HLY88630.1"
                     /translation="IAAELRDVPPAVDALTKLIALGDSAGLFDPKLVAVVRQVGAIRA
                     EQPTANVLIYTEYADSQSAAVLALREAVEGEVLSINGLDAEPERSRVADRFAERDGIV
                     VVSTDSLAEGLNLQQRCCHLIHLDLPYNPNRLEQRNGRIDRYGQTRDPQIRYLYLSGT
                     FEERLLLRLIAKYEKARAHLDVMPETLGVTADQSEYDAGLVAGFAEKQAGLFDGEPSV
                     IRTIDRVAEESGAGSYRDLLREIDRAFDGFDRSIVRHGWLGETVRDDVSANRHHSDID
                     LAGFVAAAITAETGQRQASEGILHLPPDWLNDLDGLPGFNRADGILRLTRDASMFRDT
                     KGHSLAYLGRVHPVVRRACASVQRGYEGGFDRRVSVAATVTDTPAVLLTYAAELRSKR
                     QCEFRWVFAVLLLASGPAVEVARPEEWLHWPERGTDAGWRETCAQWLPTRLPVAEAAA
                     NAAMHREAARLSAMHQGIYCRETEQLAAWLRCRADEICGKFVPRTNDLFGDEPSGPDW
                     QQHAEPLDRLVGFAADPNRPAAPRREAAGVVALYQRRDEDLAAYGCLSPPALSPLGML
                     MLVP"
     gene            1712..>3180
                     /locus_tag="VKQ27_06570"
     CDS             1712..>3180
                     /locus_tag="VKQ27_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88631.1"
                     /translation="MTFDLSDCTFVGPAFPAPFVHFELTEHLSRLDLLPTTCAWKPFR
                     QQLRPAGGAQRVCNHVIVPLASNLGFGSPVRQGDVSTREGAEDGGWLLNAPCGTRLRA
                     WAFADDTDLDAPSRTARAYRFSPTRSAFRVLLASEERLGLLTDGLELRLLICDPVRPD
                     SHIAIPLGAVDGWRNRDTVPDSFRLVLAMASPPGIAALPELLNAARLSQARLTKDLRA
                     QARHAIEGFLQGVIDRASSRQRLSPDTLWREGLALVYRLLFILKLESATEPGRGFSFA
                     STDLWRTLLSPNRALGPLVRRVIDHGQDTGRMLEDGLRLLFHVFRDGLSTSELSIAPL
                     GGALFGNGTTPLLDSLAWGERACALLLDRLLWTRPKGRGRERVHYGSLDVEELGHIYE
                     ALLELEPGIAAATMSRIRRAKIEIVVPTAIGAGYRDNPGSGTRAIWVEDIPDGSFYLR
                     VGAGRKASGSYYTPNAFVRFLVREALDPTIACCSSDDEP"
BASE COUNT          542 a          983 c         1050 g          605 t
ORIGIN      
        1 cgattgccgc cgaactgcgg gatgtgccgc ctgcggtcga tgcgctcaca aaacttatcg
       61 cgctgggaga cagcgcgggg ttgttcgatc cgaagctcgt cgcggtggtg cgccaagtgg
      121 gtgcaatccg tgcggagcag ccaacagcca acgtgctcat ttacaccgaa tacgcggaca
      181 gccagtcagc agcggtgctc gcactgcggg aagcggttga aggcgaggtg ctgtcgatca
      241 acggtctcga tgccgaaccc gagcgttcgc gcgttgccga tcggtttgcc gagcgggatg
      301 ggatcgtcgt ggtcagtacg gattcgttgg cagaagggct gaacctgcag cagaggtgct
      361 gtcatctgat ccatctggac ctgccctaca atccgaaccg actggaacag cgcaacggac
      421 gcatcgaccg ctacggccag acccgcgatc cacagatccg ttacctgtat ctctccggca
      481 cgttcgagga acggctgctg ttgcgtctga tcgccaagta cgaaaaggcg agggcgcacc
      541 ttgatgtgat gcccgaaacg ctgggcgtga ccgcggatca gtcggagtac gacgccgggc
      601 tggtcgcggg gtttgccgag aagcaagccg gcctgttcga tggtgagccg tcggtgatcc
      661 gcaccatcga tcgcgtggcg gaggaatccg gcgctggttc ctatcgcgat ttgctgcggg
      721 agatcgatcg cgcgttcgat ggcttcgatc ggtcaattgt gcggcacggc tggctcggtg
      781 agacagttcg cgatgacgta tccgcgaacc gccaccacag cgacatcgac ctcgcgggtt
      841 tcgtcgcagc ggcgataacc gccgagacag gtcagcgaca ggcgagcgaa ggcatcctgc
      901 atttgccgcc ggactggctc aatgatctcg atggtctgcc gggattcaac cgtgcggatg
      961 gcatcctgcg attgacccgg gacgcatcga tgttccgcga taccaaggga cattcgctgg
     1021 cctatctcgg ccgggtgcat cccgtggtgc ggcgagcctg cgcctcggtg cagcgcggct
     1081 acgagggtgg attcgatcgc cgtgtcagcg tggcagcaac cgtcaccgat acgcccgcgg
     1141 tgttgctgac ctacgcggca gaactccgaa gcaagcggca gtgtgagttc cgctgggttt
     1201 tcgcagtgct gctgcttgcc agcggtccgg cggttgaggt tgcccggccg gaggagtggc
     1261 tgcattggcc ggagcggggc actgacgctg gctggcgcga gacgtgtgcg cagtggcttc
     1321 cgacgcgcct gccggtggca gaggcggctg ccaatgccgc catgcatcgc gaggctgcgc
     1381 ggctgtcggc catgcatcag ggtatatact gccgtgaaac ggaacaattg gcggcctggc
     1441 tgcgctgccg cgccgacgaa atctgcggca aattcgtgcc acgtaccaac gacctgttcg
     1501 gcgatgaacc atcgggtccc gattggcaac aacacgccga gccgctggac aggttggttg
     1561 gatttgctgc ggatcccaac aggcctgccg cacctcggcg agaagccgcc ggagttgtcg
     1621 cgttgtacca gagacgcgac gaagatctcg cggcatacgg ctgtttgtcg ccaccggctc
     1681 tcagtccgct cggcatgctg atgcttgtgc catgacattc gacctcagcg actgcacctt
     1741 tgtcggtccc gcgttcccgg caccgtttgt tcatttcgaa ctcaccgaac acctgagcag
     1801 gcttgacctg ttgccgacca cgtgtgcctg gaagccattt cgccagcagc ttcgtccggc
     1861 cggcggcgcg caacgggtct gcaatcacgt catcgtgcct ctcgcatcga acctaggctt
     1921 cggatcaccc gttcgtcagg gcgatgtcag cacacgcgaa ggcgccgagg atggcggctg
     1981 gctgctgaac gcaccgtgcg gcacgcggct gcgcgcctgg gcgtttgccg acgatactga
     2041 tctcgatgcg ccaagccgca ccgcccgggc atatcgcttc agcccgaccc gcagcgcgtt
     2101 tcgagtcctg cttgccagcg aggaacgcct gggcctgttg accgacggcc tggagctccg
     2161 cctgctgatt tgcgacccgg tgcgcccgga cagtcatatc gccatcccgc tcggcgcggt
     2221 cgatggctgg cgcaatcgcg atacggtccc agattcattc cgcctggtac tcgcgatggc
     2281 atcaccgccc ggtattgccg cgctgcccga attgctgaac gcggcacggc tcagccaggc
     2341 gagactgacc aaggacctac gcgcgcaggc gcggcatgca atcgagggat tccttcaagg
     2401 ggtgatcgat cgcgccagct caagacaacg attgtctccc gatacattgt ggcgcgaggg
     2461 actggcgctg gtttatcgcc tgctgttcat cctgaagcta gagagtgcca ccgaaccggg
     2521 acgcgggttc agctttgctt ccactgatct gtggcgcacg ttgctttcgc ccaatcgcgc
     2581 gctcggaccg ctggtacgtc gcgtgattga ccacggtcag gacacgggcc gcatgctgga
     2641 ggacggtctg cggctgctgt tccacgtgtt ccgtgatggg ttgtccacca gcgaactttc
     2701 gatcgcgccg ctaggtggcg cgttgttcgg caacgggacg acaccgctgc tggacagtct
     2761 ggcgtggggc gaacgggcct gtgcgttgct gcttgatcga ctgttgtgga ccaggccgaa
     2821 gggtaggggg cgcgaacgcg tgcattacgg ctcgctcgat gtcgaggagc tgggccacat
     2881 ctatgaggcg ttgctggaac tcgaaccggg catcgccgcg gcaaccatgt cgcggatccg
     2941 ccgcgcgaag atcgaaatcg tggtgccgac ggctatcggt gctggctatc gtgacaaccc
     3001 tggcagcggc acgcgcgcga tctgggtaga ggacatcccc gacggctcct tctacctccg
     3061 cgtcggcgcg ggccggaagg caagcggttc gtactacacg ccaaatgcct ttgtgcgctt
     3121 cctcgtgcgc gaagcgctgg accccacaat cgcatgttgc tcctctgacg atgagcccca
//
LOCUS       DATGEM010000207         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_58865, whole genome shotgun sequence.
ACCESSION   DATGEM010000207 DATGEM010000000
VERSION     DATGEM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58865"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VKQ27_06575"
     CDS             complement(<1..295)
                     /locus_tag="VKQ27_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640034.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLY88632.1"
                     /translation="MLKRPIIGVTLDSEQPGGYSKYPWYAIRQNYADAIIAAGGLPIA
                     LPHDASLAPAYLDSIDALVVTGGAFDVHPSLYGEADMHATVTLKENRTDAELAL"
     gene            complement(305..871)
                     /locus_tag="VKQ27_06580"
     CDS             complement(305..871)
                     /locus_tag="VKQ27_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY88633.1"
                     /translation="MPRIPLVTETSMTADQRRVYDAMMNSPRRSPPVGPLAAAMHRPD
                     LAEAWSNLGLVLRFNSSFPPRLLEFIILLTGRFWDCQFEWLSHEAEAREAGLSEQTIE
                     ALRQGGSTFAQQDEQAIHDYAIELLRHHRAADATYQRILTAYGTTGIVELTALIGYYA
                     MVALTLNAHDIGVPEGTTPPLPKLGPRG"
     gene            909..1361
                     /locus_tag="VKQ27_06585"
     CDS             909..1361
                     /locus_tag="VKQ27_06585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLY88634.1"
                     /translation="MVAGFRPMEIALEEARAAAAHGEVPVGAVVLDATGAVLARAGNE
                     VEARSDASAHAEMLVLRAAAAARGKPRLPDCDLVVTLEPCPMCAQAASLFRVRRLVFG
                     AYDPKGGGVEHGARVLDAASCMWRPEVIGGVREAEAATLLREFFGERR"
     gene            1427..1804
                     /locus_tag="VKQ27_06590"
     CDS             1427..1804
                     /locus_tag="VKQ27_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88635.1"
                     /translation="MRTTSVLAAAFVLAGCATVHRMSADEVRATFVGMTDYGTAQNGM
                     PFVAYLSPDGAVRFQREGESDTGRYRIEPDGTMCVAYQNYQQGQDVCQSMWKGADRYY
                     SSLSNGQPGVTITAIKPGNAENL"
     gene            complement(1818..2201)
                     /locus_tag="VKQ27_06595"
     CDS             complement(1818..2201)
                     /locus_tag="VKQ27_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY88636.1"
                     /translation="MDQIQNIASAINGYFDLMYDADDNRFPDVFHDTCLIHGLRDGTL
                     TAWSASEFRDVMRGRPSPASMKSPRDQEILSIEHTAPALAAAKVRVRIGQTCFIDYLI
                     FHCIEGTWRVTSKAFHIARVFPAGS"
     gene            2305..3282
                     /locus_tag="VKQ27_06600"
     CDS             2305..3282
                     /locus_tag="VKQ27_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY88637.1"
                     /translation="MDDIDSRLVGGLIVLAAIADAGGFGRAAERLGMTQPGVSKAIAR
                     LEARVGARLVERTSRAIALTDAGRVLHERVVPHLAAIGEAATETVQSQGAVRGRLRVN
                     VDPLFSRMVLSPKLSEFLLRNPGLELRIETKDRIADLVSDGFDLAVRFGEPAPSALIA
                     RRLLDARILTVASPAYIERHGRPGRPQELATDAHECIHAIDPSTGHRFDWEFRRGGEI
                     IPIAVNGRLTVTDAGTKLGACLAGFGIAQVIDLGLEQHLGSGALINLFPDWSEERFPL
                     YVYYVSRNHVPAKVRAFIDFIVGSFSANIEPASPGGARPGATLGVTETN"
     gene            complement(3285..>3951)
                     /locus_tag="VKQ27_06605"
     CDS             complement(3285..>3951)
                     /locus_tag="VKQ27_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HLY88638.1"
                     /translation="ALPLGAAQDHKRVGEGESGPNTGGMGAYSPTPSFPPAAQQAAMD
                     GIIRPALAEMARRGTPFRGILYAGLMLTGEGAKLIEFNVRFGDPECQVLLPRLMSDLL
                     PALQAAYDGELQNFDLRWRDATCITVVMAARGYPDAPERGSEIRGLQRAVAEPNVLVF
                     HAGTEADAQGHLRAAGGRVLNVCATGPNVRRARDAAYAAIKLIDWPEGFCRHDIGWRA
                     LAP"
BASE COUNT          673 a         1156 c         1406 g          716 t
ORIGIN      
        1 tgagggctag ttcggcgtcg gtgcggttct ccttcagcgt gacggtggcg tgcatgtcgg
       61 cctcgccata aagcgaggga tgcacatcga acgcgccgcc ggtgaccaca agcgcatcga
      121 tgctgtccag gtaggcgggc gccaagctcg catcgtgtgg cagggcgatc ggcagaccgc
      181 cggcggcgat gatcgcgtcg gcatagttct ggcggatcgc ataccagggg tatttggagt
      241 agccgccggg ctgctcggaa tcgagagtga cgccgatgat gggacgcttg agcatggtcc
      301 cgtgctatcc ccgtgggccg agcttgggca agggcggcgt ggtcccctcg ggcacgccaa
      361 tgtcgtgcgc gttcagggtg agggcgacca tcgcgtagta gccgatgagc gcggtcagtt
      421 cgacaatgcc ggtcgtgccg taggcggtga ggatgcgctg ataggttgcg tctgcggcgc
      481 gatggtgccg cagcagctcg atggcgtagt cgtggatcgc ctgttcgtcc tgctgggcga
      541 aggtggagcc gccttggcgg agggcttcta tcgtctgctc ggagaggccg gcctcgcggg
      601 cctcggcttc gtgggagagc cactcgaact ggcaatccca gaagcggccg gtcagcagga
      661 tgatgaactc gagcagccgc ggcggaaagc ttgagttgaa gcggagcacc aagccgaggt
      721 tggaccatgc ctcggcgagg tcggggcggt gcatggcggc tgcaagcggg cccaccggcg
      781 gcgagcggcg cgggctgttc atcatcgcgt cgtagacccg acgctggtcc gccgtcatgc
      841 tggtctcggt gaccaggggg atgcggggca tttctgcgtc ctccgatttg tggggggttc
      901 agtctgccat ggtggccggc tttcgtccga tggagatcgc gctggaagag gcgcgcgcgg
      961 cggcggcaca tggcgaggtg ccggtcggcg cggtcgtgct ggatgctact ggggcagtgc
     1021 tggcgcgcgc cggcaatgag gtcgaggcgc ggagcgatgc ctcggcacat gccgagatgc
     1081 tggtgctgcg agcggcggcg gcggcccggg ggaagccccg gctgccagac tgtgacctgg
     1141 tggtgactct ggagccatgt ccgatgtgcg cgcaggcggc aagccttttc cgggtaaggc
     1201 ggctggtgtt cggtgcctat gacccgaagg ggggcggggt ggaacacggc gcgcgggtgc
     1261 tcgatgcggc ttcgtgtatg tggcggcccg aggtgatcgg cggcgtgcgc gaggcggagg
     1321 cggcgacgct gctgcgggag ttcttcggcg agaggaggta gccggttcct gggtcagact
     1381 cgcggtgcac tgcatagtgg cggcctccac cgatcaggca ggtttcatgc gcaccacgtc
     1441 agtcctcgcc gccgcctttg tccttgccgg ctgcgccacc gtccaccgca tgtccgccga
     1501 cgaagtgcgc gcaacgttcg tcggcatgac tgattatggg acggcgcaga acggtatgcc
     1561 gttcgtcgct tatctatcgc cagacggcgc ggtgaggttc cagcgcgaag gtgagagcga
     1621 cacgggtcgc tatcgcatag agcctgacgg cacgatgtgt gtggcatacc agaactatca
     1681 gcaaggccag gacgtttgcc agagcatgtg gaagggcgcg gaccgctact attcctcgtt
     1741 gtcgaatggt cagcccggcg tcaccatcac cgcgatcaaa ccaggcaacg ccgaaaatct
     1801 ctgattgcta ggggcggcta tgatcctgcc gggaagacgc gcgcgatgtg gaacgccttg
     1861 gacgtgacac gccatgtccc ctcgatgcaa tggaatatca gataatcaat gaagcaggtc
     1921 tggccgatcc gcacgcgtac tttggcggct gccagtgcgg gcgcggtgtg ctcaatgctg
     1981 agaatctcct ggtcgcgcgg cgatttcatc gacgccggcg acggccggcc gcgcataacg
     2041 tcacggaatt cggatgcaga ccaggcggtt agcgtaccgt cgcgcaagcc gtggatcaga
     2101 caggtatcgt ggaaaacgtc agggaagcgg ttgtcgtccg cgtcatacat caggtcgaaa
     2161 tagccattga tcgcggatgc gatattttgg atctggtcca tacaagtctc caacagagga
     2221 aatgcacaac gaccgtggcc caatgtgacg gcggagcacc atccgttcgg gtgtcatccc
     2281 ttggatgact gagaggcatc aacgatggac gacatcgaca gtcggttggt tggaggtctg
     2341 atagtcctcg cggccattgc cgacgcgggc ggctttggtc gggccgccga acggctgggc
     2401 atgacgcagc ccggcgtgag caaggcgatc gcgaggctcg aggctcgcgt cggtgccagg
     2461 ttggtcgagc gcacgagccg tgccattgcg ctcaccgacg caggacgcgt gctgcacgaa
     2521 cgggtcgttc ctcacctggc ggcgatcggt gaggcggcga cagaaaccgt tcaatcgcaa
     2581 ggtgcggtgc gcggcaggtt gcgggtcaat gtcgatccgc tgttctcacg catggttctg
     2641 tcgccgaagc tgagcgaatt cctgctgcga aatccaggcc tggagcttcg catcgaaacc
     2701 aaagaccgaa tcgcagacct agtcagcgat ggcttcgatc tggcggtccg cttcggggag
     2761 ccggcaccat cagccctgat cgcccggcgt ctgctcgatg cgcgcatcct gacggttgct
     2821 tcgcccgcat atatcgaacg acatggaaga ccgggacggc cgcaggagct ggcgaccgat
     2881 gcgcacgaat gtatccacgc gatcgatcct tcgacagggc atcgcttcga ctgggagttc
     2941 cggcgaggcg gcgagatcat tcctattgcg gtcaatggca ggctaaccgt gaccgatgca
     3001 gggacaaagc tcggagcctg tctggcaggc ttcggcattg cgcaggtgat cgacctcggg
     3061 ctggagcagc atctcggcag cggcgcgttg atcaacctgt tccccgactg gtcggaggag
     3121 cggtttccgc tatacgtcta ttacgtttcc cggaatcacg tgccggcaaa ggtgcgggcg
     3181 ttcatcgact tcatcgtcgg ctcgttcagt gcgaacatcg aaccggcttc gccaggtggt
     3241 gcgcgccccg gagcgacact gggggtgaca gaaactaact gaacctaggg cgcgagtgcg
     3301 cgccaaccga tgtcgtgccg gcagaaacct tctggccagt caatcaactt gatcgcggca
     3361 taggcggcgt cgcgagccct tcgcacgtta ggtccggtgg cacagacgtt cagcacgcgg
     3421 ccaccggcgg cgcggaggtg tccctgggca tctgcttcgg tgccggcgtg gaacaccagc
     3481 acgtttggct cggcaaccgc gcgctgcagg ccacggattt cgctgccgcg ctcgggcgcg
     3541 tcgggataac cgcgcgcggc catcacgact gtgatgcagg tcgcatcgcg ccagcgcagg
     3601 tcgaagttct gcagttcgcc gtcgtacgcg gcctgcaacg cggggagcag gtcggacatc
     3661 agtcgcggca gcaggacctg gcactccggg tcgccgaagc ggacgttgaa ctctatcagt
     3721 ttcgcgcctt cgcccgtgag catcaggcca gcatagagga tgccgcggaa tggtgtgcca
     3781 cgccgcgcca tctcagcgag agcggggcga atgatgccgt ccatcgctgc ctgttgtgcc
     3841 gccggaggaa atgacggcgt tggcgagtag gcacccatcc ctccggtgtt cggcccactc
     3901 tcgccttcgc cgacgcgctt gtgatcctgc gcggcgccaa gcggcagggc g
//
LOCUS       DATGEM010000208         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_828038, whole genome shotgun sequence.
ACCESSION   DATGEM010000208 DATGEM010000000
VERSION     DATGEM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828038"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VKQ27_06610"
     CDS             complement(<1..1109)
                     /locus_tag="VKQ27_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88639.1"
                     /translation="MDAQTIQRRRWLILSVLIVGLLAIVIDNTVLNVALKTIAEPRGG
                     LGASQSQLEWAINSYTLVFAGLLFTFGVIGDRIGRKKMLLVGLLLFGIASVLSAYAHN
                     PTELIWARAAMGLGGAAVMPQTLSIISQVFEPAERPKAIGLWAMAVGIGVALGPVLGG
                     LLLSHFWWGSVFLINVPVVIFGLIASIALIPESKNKNAGGIDVAGVLLSIVGLVALVY
                     GIVEGGDQASWVHLKVLGPIIGGLVILAVFAWYETRIAHPSLDVRLFKDRRLSASVGA
                     VALAFFSMGGVFFFMSFYLQNVRGYSALDAGLLTVPFAFGQLLLSPRSAGLVRRYGAR
                     AVGAAGMATITVVIAGYLLMGISTPIAVLCVLFAL"
     gene            1314..1541
                     /locus_tag="VKQ27_06615"
     CDS             1314..1541
                     /locus_tag="VKQ27_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88640.1"
                     /translation="MKPARAAPRRFLPGSPFNSLHEDPPIEQFAVRDQVSHDKYGLGR
                     VVLVEDDEAIVVDFGSCNIRICSPFARLAKL"
     gene            complement(1548..1838)
                     /locus_tag="VKQ27_06620"
     CDS             complement(1548..1838)
                     /locus_tag="VKQ27_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY88641.1"
                     /translation="MASWTFLTAHTRVLLCIVRDPEVRLRDIAASLDITERTAFAIIT
                     DLTEAGFVVKEKDGRRNRYRIRTELPLPESAARDRTVGELLALLAGADETAP"
BASE COUNT          417 a          714 c          650 g          317 t
ORIGIN      
        1 tgcagtgcga acagcacgca cagcaccgcg atcggcgtgc tgatccccat cagcaggtag
       61 cccgcgatga ccaccgtgat cgtcgccatt ccggcggcac cgactgcccg ggcgccgtaa
      121 cgccgcacca gcccggcact gcgcggcgac agcagcagct ggccgaacgc gaacggcacc
      181 gtgagcagcc ccgcgtccag cgccgagtag ccgcggacgt tctgcaggta gaagctcatg
      241 aagaagaaca cgccgcccat gctgaagaac gccagcgcca ccgcgccgac cgacgcggac
      301 agcctgcggt ccttgaacag ccgcacgtca agcgacgggt gcgcgatcct ggtctcgtac
      361 caggcgaaca ccgccaggat gaccaggccg ccgatgatcg ggccgagcac cttcaggtgc
      421 acccaggacg cctggtcgcc gccctcgacg atgccataga ccagcgcgac caggccgacg
      481 atggacagca gcacaccggc gacgtcgatg ccgcccgcgt tcttgttctt cgactcaggt
      541 atcagggcga tcgaagcgat cagcccgaaa atcacgactg gcacgttgat caggaagacc
      601 gaaccccacc agaagtgaga cagcagcagc ccgccgagca ccggcccaag cgcgacgccg
      661 atacccacgg ccatcgccca caagccgatc gccttcggcc gctcagccgg ctcgaacacc
      721 tgcgagatga tggacagcgt ctgcggcatg accgccgcgc cgcccaggcc catggcagcc
      781 cgcgcccaga tcagttcggt cgggttgtgc gcataggcgg aaagcacgct cgcgatgccg
      841 aacaacagca agccgaccag cagcatcttc ttgcggccga tccggtcgcc gatcacgccg
      901 aaggtgaaca gcagcccggc gaacacgagc gtgtacgagt taatggccca ctcgagctga
      961 gactggctgg cgccgagacc cccacgcggc tcggcgatag tcttcagcgc gacgttgagc
     1021 acggtgttgt cgataacaat ggcgagcaat cccacgatca gcacgctgag aatcagccag
     1081 cgccgccgtt gaatcgtctg tgcgtccacc cgtacccctt cttccactct tgactatcga
     1141 tacggaacac acccgtatcg gaatagattc cgcagtggat tgtgacctgc gtcacatatg
     1201 gggccgggca gcaacgtaac ggtgcgtgct cgcggcctcc tgcgccggca tctggccaga
     1261 taggtctgcc aggaccggcg ccgccgcggc cgcgagggcg catactggaa ttaatgaaac
     1321 ctgcccgcgc cgcgccccgg cgcttcctgc ccggaagtcc cttcaactcg ctgcacgagg
     1381 acccgcccat cgagcagttc gccgttcgcg atcaggtcag ccacgacaag tacggcctcg
     1441 gccgcgtggt cctagtcgag gacgatgagg cgatagtcgt cgacttcggt tcgtgcaata
     1501 tccggatctg ctcgcctttc gccaggctgg ccaagctctg accatgctca cggcgcggtc
     1561 tcgtccgcac cggccagcag ggccagcaac tcgccgacgg tacgatcccg ggcggctgat
     1621 tcaggcagcg ggagttctgt ccgaatacgg tagcgattgc ggcggccatc cttctccttg
     1681 accacgaatc cagcctcggt caggtccgtg atgatggcga aggcggtgcg ttcggtgatg
     1741 tcgagacttg ccgcgatatc ccggagacgt acctccgggt cacggacgat acagagcaga
     1801 accctggtgt gcgcggtgag gaaagtccag ctagccacga aggtaagtta ccgcattccg
     1861 gaaaaatctt gctagttttc aggtgacggt atacgtttgg gcccaggtac ccggacgtag
     1921 acgccagacg gcggcaagct tccggggctt ctggaggagc gcatgcagcc ggtcggccgc
     1981 ttgctgaggc agtccggcgc cgcgacctcc ggcggtgcct ccgccgaccc ggtcagcgac
     2041 tacctcaagc agatcagcaa ggtgcggctg ctcagcgcgg accaggaagt acagctcg
//
LOCUS       DATGEM010000209         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_482119, whole genome shotgun sequence.
ACCESSION   DATGEM010000209 DATGEM010000000
VERSION     DATGEM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482119"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..310
                     /locus_tag="VKQ27_06625"
     CDS             5..310
                     /locus_tag="VKQ27_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HLY88642.1"
                     /translation="MRARIYQPPKTAMQSGWAGTHEWMLEFEPTSPRRADPLMGWIGS
                     NDTQAQVKLTFTTREEAVAYADRNGIAYDLELPQPRRVKPKAYADNFRYGRIENWTH"
     gene            complement(307..1644)
                     /locus_tag="VKQ27_06630"
     CDS             complement(307..1644)
                     /locus_tag="VKQ27_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88643.1"
                     /translation="MLVVALGTLVVPFDSSVNFAFPFITRAFGLPIPAIQWVIISYTL
                     TYAALMLVFGRVGDMLGYRRIFLIGSAWSSVAFVLCAMAPSFAALLVARVLQGVGAAL
                     VLSCGPALATSLHHESARTRVLGIYTMVIGIGGALGPLVAGMLVPLIDWPAVFWFRAP
                     LALAAFLLGWLLPRDTRPTHQEKFDALGGVLLVLAISAMLLALNQLQRSAAVTALAAI
                     LCAAATAGFIIQERSTERPIMDLRFFRDLDFSLLNAGHAALNLAGFSVLLLVPFYLAR
                     FGGLGAHAIGVLLACSPTGTVIAAPLAARLATRVAPRLLAVIGASAMAIGQVLIGTAG
                     AVPNVPALAGAMALQGFGVGLFQVAYFDIVTASIPRADRGVAGSLVMMTRTLGTVSGA
                     SVLILLFQTWAGSAPDAQAFLAGFGNTFRLAAALPLLVVVLGLARGWGRPRAA"
     gene            complement(1685..1954)
                     /locus_tag="VKQ27_06635"
     CDS             complement(1685..1954)
                     /locus_tag="VKQ27_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLY88644.1"
                     /translation="MSKAFIAQVLQESASLTGAAANRAAGELMDAVVKSLKKEGKFTL
                     PSFGTFTVRKTKARKGLNPRTGESIKVKAGKTVRFKASPSLKKAV"
     gene            complement(2106..2822)
                     /gene="phoB"
                     /locus_tag="VKQ27_06640"
     CDS             complement(2106..2822)
                     /gene="phoB"
                     /locus_tag="VKQ27_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HLY88645.1"
                     /translation="MADVASSLSKPLVLVVEDEAALATMLRYNLEKQGFRVEEAADGQ
                     EALTRIAETQPDLVLLDWMLPQMSGIEVCRQIRRRSSTRDLPVIMVTARTEDQDAVRG
                     LNTGADDYITKPFSMESLLARMRALLRRSNAMPTKGLLAFHDITMDLAAHRVQRNNRP
                     IHLGPTEFRLLEFFMQHPKRVFSREELLDAVWGPDIHVEPRTVDVHIRRLRKSINAKD
                     ELDIVRTVRAAGYALDTEPV"
     gene            complement(2831..>3217)
                     /locus_tag="VKQ27_06645"
     CDS             complement(2831..>3217)
                     /locus_tag="VKQ27_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HLY88646.1"
                     /translation="IVLAQYSLPYSLAGLAHMARMVQGQLKSIIDALGDNDAEKAVEV
                     WRSDQVIDDIYNAIFRELITYMMEDPRNITPCTHLLFIAKNLERIGDHATNIAENLYY
                     AVKGESLPESRPKGDTSAYAVVRPRE"
BASE COUNT          654 a         1054 c          995 g          514 t
ORIGIN      
        1 atttatgcgc gcgcgcatct accagccgcc gaagacagcg atgcaatccg gctgggccgg
       61 cacgcatgaa tggatgctcg agttcgagcc gacgtcacca cgtcgggccg atccgctgat
      121 gggctggatc ggcagcaacg acacccaggc gcaggtgaag ctgaccttca ccacgcgcga
      181 ggaggcggtg gcgtacgccg acaggaatgg tatcgcctac gacctggagc tgccgcagcc
      241 gcggcgcgtg aagccgaagg cgtatgccga caatttccgc tacgggcgga ttgagaactg
      301 gacgcactag gccgcccgtg gccgtcccca cccccgcgcc aatcccagca ccacgaccag
      361 cagcggcagt gccgccgcca gcctgaaggt attgccgaac ccggccagga acgcctgcgc
      421 gtccggtgcc gaacccgccc aggtctggaa cagcaggatc agcacgctcg cgccactcac
      481 cgtgccgagc gtacgcgtca tcatcaccag gctgccggcc acgccgcgat cggcacgcgg
      541 aatcgatgcc gtgacgatat cgaaataggc cacctggaac agccccacgc cgaaaccctg
      601 caacgccatg gcgccggcca gcgcgggcac gttcggcact gcgccggcgg taccgataag
      661 gacctgtccg atcgccatcg ccgacgcgcc gatcaccgca agcaggcggg gcgcgacgcg
      721 cgtggccaat cgcgcggcga gcggtgcggc gatgaccgtg cccgtcggtg aacaggccag
      781 cagcaccccg atcgcatgcg cgccaaggcc gccgaaccgg gcgagataga acggcaccag
      841 cagcagcacc gagaaccccg caaggttgag cgctgcgtgt ccggcattca gcagtgaaaa
      901 gtcgagatcg cggaagaacc gcaaatccat gatcggccgc tcggtggatc gttcctggat
      961 gatgaaacca gccgtcgccg cagcgcacag tattgccgcg agcgcagtaa cagcagcgct
     1021 gcgctgcagc tggttcaacg ccaacagcat cgcactgatc gccagcacca gcagcacgcc
     1081 gcccagcgca tcgaatttct cctgatgcgt cggccgcgta tcgcgtggca gcagccatcc
     1141 aagcaaaaaa gccgccagcg ctagcggcgc gcggaaccag aacaccgccg gccaatcgat
     1201 cagcggcacc agcattccgg caaccagcgg gcccaacgcg ccaccgatgc cgatcaccat
     1261 cgtgtagata ccgagcaccc gcgtgcgcgc cgattcgtgg tgcagactgg tcgccagcgc
     1321 cggtccacaa ctcaacacca gcgccgcgcc aacgccttgc agcacgcgcg cgaccagcag
     1381 cgcggcaaag ctcggtgcca tcgcgcacag cacgaaggca accgaactcc acgcgctgcc
     1441 gatcaggaag atgcggcgat aaccaagcat gtcaccaacc cgcccgaaca ccagcatcaa
     1501 cgcggcgtag gtcaacgtgt aggagatgat gacccactgg atcgccggta tcggcaggcc
     1561 aaacgcacgc gtgatgaatg gaaaggcgaa gttgacggac gaatcgaacg ggacaaccag
     1621 cgtgccgagt gcgaccacca gcacgccgaa gcccgcacgc gaagctgctg gtggtgacgc
     1681 ggccttagac cgccttcttc agcgaaggcg aggccttgaa gcgcaccgtc ttgccagcct
     1741 tcaccttgat cgattccccg gtgcgaggat tcagtccctt gcgcgccttg gtcttgcgca
     1801 cggtgaacgt gccaaagctt ggcagagtaa acttgccttc cttcttcagc gacttgacca
     1861 cggcgtccat cagttcgcca gcggcgcgat tcgcggcggc accggtcagt gatgcggact
     1921 cctgcagtac ctgggcaatg aacgcctttg acatctgtct cctcactcga attgcgttac
     1981 gcggtggtca cagcaagcga cggcgccgcg aatcgcagcg ccgttgcaac ggttctagac
     2041 ggggcgccgg agtttcgcca gcccctctac agagtttgcg atatgcgatc gcgcggcgcg
     2101 gaggatcaga ccggttcggt gtccagtgcg tagccggcag cgcgaaccgt gcgcacgatg
     2161 tccagctcat ccttggcgtt gatcgacttg cgcagccggc ggatatgcac atccaccgtg
     2221 cgcggttcga cgtgaatgtc ggggccccac acggcgtcca gcagttcttc gcgcgaaaac
     2281 acccgcttgg ggtgctgcat gaaaaactcc agcagacgga attccgtcgg acccaagtgg
     2341 atcgggcggt tgttgcgctg cacgcgatgc gcggcgaggt ccatcgtgat gtcgtggaac
     2401 gcaagcaggc ctttggtcgg catcgcattc gaacggcgca ggagggcacg catccgggcc
     2461 agcagcgact ccatcgagaa cggcttggtg atgtagtcgt cggcacctgt gttgaggcca
     2521 cggacggcgt cctgatcctc ggtgcgcgcg gtcaccatga taacgggcag gtcgcgcgta
     2581 gaagagcgtc ggcggatctg gcggcaaacc tcgatgccgg acatctgcgg cagcatccag
     2641 tcgagcagca ccagatcggg ctgggtctcg gcgatgcggg tgagagcctc ttggccgtct
     2701 gccgcttcct cgacgcggaa gccctgcttt tccaggttat aacgcagcat tgtggccaat
     2761 gccgcttcgt cttcaaccac gagtaccagc ggcttggaca agcttgacgc gacgtcagcc
     2821 atggtttccc tcactctcgt ggccgcacta cagcgtaggc agacgtatca cccttcgggc
     2881 gagattccgg cagcgactcg cccttcaccg cataataaag gttttccgca atatttgtgg
     2941 cgtgatcgcc aatacgctcg aggttttttg cgataaataa aagatgagtg cagggcgtga
     3001 tattgcgtgg gtcttccatc atgtaggtga tcagctcgcg gaagatcgcg ttgtagatgt
     3061 catcgatcac ctggtcggac cgccagactt ccacggcctt ttccgcatcg ttgtcgccga
     3121 gagcgtcgat gatcgacttc agctgcccct gcaccatgcg cgccatatgg gcgagcccgg
     3181 ccagcgaata aggtagcgag tactgcgcca gcacgat
//
LOCUS       DATGEM010000210         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_375564, whole genome shotgun sequence.
ACCESSION   DATGEM010000210 DATGEM010000000
VERSION     DATGEM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375564"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /gene="glyA"
                     /locus_tag="VKQ27_06650"
     CDS             <1..1149
                     /gene="glyA"
                     /locus_tag="VKQ27_06650"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLY88647.1"
                     /translation="VLEAQGSVLTNKYAEGYPGRRYYGGCVHVDVAENLAIERAKRLF
                     ACHYANVQPHSGAQANQAVFLALLQPGDTILGMSLAAGGHLTHGAAPNLSGKWFHAVQ
                     YGVRRDDGLLDYEELERVARAEKPKLIIAGGSAYPRFIDFPRIRRVADEVGAYFLVDM
                     AHFAGLVAADVFPSPLPHAHVVTTTTHKTLRGPRGGMILTNDPDIGRKINSAVFPGLQ
                     GGPLMHVIAAKAVAFGEALRTDFRVYQKAVVENAKVLAETLVQQGLDIVTGGTDCHLM
                     LVDLRPKRVTGKAAEASLERARITTNKNAIPFDPEKPAITSGIRLGSPAATSRGFGTA
                     EFREVGLMIDEVLEALSGSNDGFNATAEAAVGARVQELCARFPLYRTR"
     gene            1153..1608
                     /gene="nrdR"
                     /locus_tag="VKQ27_06655"
     CDS             1153..1608
                     /gene="nrdR"
                     /locus_tag="VKQ27_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HLY88648.1"
                     /translation="MRCPFCGHEDTQVKDSRPSEDGSAIRRRRFCAHCGQRFTTIERT
                     QLRELIVVKTDSRRVPFDRDKLARSIRVALNKRPVQEERIERIVNGIVRQLEASGESE
                     ISSKQIGELVMDTLKEVDPVAYVRFASVYRNFREAKDFEDFIGSLGETT"
     gene            1662..2744
                     /gene="ribD"
                     /locus_tag="VKQ27_06660"
     CDS             1662..2744
                     /gene="ribD"
                     /locus_tag="VKQ27_06660"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLY88649.1"
                     /translation="MRSALALARRGLGSTWPNPSVGCVIVKDGRVVGRAVTAPGGRPH
                     AEPLALDLAGASARGATVYVTLEPCCHWGRTPPCTDALISAGVARVVIATRDPDPRVD
                     GAGIARLRAAGIVVDEGVLNHSADAIAAGFWSRIQSGRPLVTLKLATTLDGRIATRTG
                     ESRWITGEPARRAAHALRGRSDAVMVGIGTVMADDPELTCRVPGFRTTPLVRIVADSH
                     LRTPLTKKLIATASESPTWILIRNGTDRERRHAFAELGIRLIEVTGAQTGVDITQALS
                     ALGSAGLTRILVEGGAELAASLLRADLVDRIAWFHAPTVMGGDAWPAVQAFGIDHLAA
                     MPRFTRVAETVLGDDMLTEFARRTEQ"
     gene            2934..3533
                     /locus_tag="VKQ27_06665"
     CDS             2934..3533
                     /locus_tag="VKQ27_06665"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HLY88650.1"
                     /translation="MFTGIVTALGTVRAITPLATGSDMRLTIEAPWPDAASIPIGASI
                     GCSGCCLTAVEVGRDWFTADASAETLSRTTLGRWRPGTRVNLERSLCLGDELGGHLVS
                     GHVDGVGEALSSVPEHGSARWTFRVPPALARFIAVKGSIGVDGVSLTVNEVSDDTFGV
                     NIIPHTASVTSFGSLRPGDAVNLEIDMLARYVARLAEFK"
     gene            3534..>4644
                     /gene="ribB"
                     /locus_tag="VKQ27_06670"
     CDS             3534..>4644
                     /gene="ribB"
                     /locus_tag="VKQ27_06670"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HLY88651.1"
                     /translation="MSQNLKTVELSRYLSAADELIEEARNGRMFILVDDEDRENEGDL
                     IVPAQFATPDAINFMARHARGLICLALTRQRVEQLGLPLMAAHNGTRHQTAFTISIEA
                     REGVTTGISAHDRARTIAVAINPEAGRDDIVTPGHVFPLMAREGGTLVRAGHTEASVD
                     IARKAGLYPAAVICEIMNDDGTMARLPDLVAFAQRHNLKLGSIADLIAHRRLTERLVK
                     RVAESPFQHVAGGEWRAVVYANTVEYQEHLVLVKGDVSGNDPVMVRMHAVDLLDDMTG
                     GPHWIGIHNAMHMISKEDRGVVVLIREHRKTALAERVGQLAAGDRRPQRELRDYGIGA
                     QILLDLGVKNMVLLSNSRRTIIGLDGYGLNIVAQKP"
BASE COUNT          802 a         1645 c         1426 g          771 t
ORIGIN      
        1 gtgctcgagg cgcaaggctc ggtgctgacc aacaaatacg ccgagggcta tcccggccgg
       61 cgctactacg gtggctgcgt ccacgtcgat gtggccgaga acctggccat agagcgcgcc
      121 aagcggctct tcgcctgcca ttacgccaac gttcagccgc actccggtgc ccaggccaac
      181 caggcggtct tcctggccct gctgcaaccc ggcgacacca tccttggcat gagcctcgct
      241 gctggcggcc acttgaccca cggcgccgcg cccaacctgt ccggcaagtg gttccacgcg
      301 gtgcaatacg gggtccggcg cgacgacggc ctgctcgact acgaagaact cgaacgcgtg
      361 gcgcgcgccg aaaagccaaa gctcatcatc gcaggtggct ccgcgtatcc gcgcttcatc
      421 gatttcccgc gcatccgtcg cgtggccgat gaggtgggcg catacttcct ggtcgatatg
      481 gcgcatttcg ccggcctggt tgcggccgac gtgttcccga gcccgctgcc gcatgcccat
      541 gtcgtcacga ccaccacgca caagaccctt cgcggccccc gcggcggcat gatcctgacg
      601 aacgacccgg acatcggccg caagatcaac agcgccgtct tcccgggcct gcaaggcggg
      661 ccgctcatgc acgtcatcgc cgccaaggcc gtcgccttcg gtgaggcgct gcgcaccgac
      721 ttccgcgtct accagaaggc ggtggtcgaa aacgccaagg tcctggccga gacactggtg
      781 cagcaggggc tcgacatcgt caccggcggt accgactgcc atctgatgct ggtcgatctc
      841 cgccccaagc gcgtcaccgg caaggcggcg gaggccagcc tggagcgcgc ccgcatcacc
      901 accaacaaga acgccattcc gttcgatccc gagaagcctg cgatcacctc gggcatccgc
      961 ctcggctcgc ccgcggccac gtcccgcggc ttcggcaccg ctgaattccg cgaagtgggc
     1021 ctgatgattg acgaggtgct ggaagccctg tcgggttcga acgacggctt caacgccacg
     1081 gccgaagccg ccgtcggtgc cagggtgcag gaactctgcg cccgcttccc tctctatcgc
     1141 acgcgctaac ccatgcgctg tcccttctgc ggccatgaag acacccaggt gaaggacagt
     1201 cgtcccagcg aggacggcag cgccatccga cgccgtcgct tctgcgccca ttgtggtcag
     1261 cgcttcacca cgatcgaacg cacccagttg cgcgagctta tcgtggtgaa aactgacagc
     1321 cgccgggtgc cgttcgatcg cgacaagctg gcgcgctcga ttcgcgtcgc cttgaacaag
     1381 cggccggtgc aggaggagcg tatcgagcgt atcgtcaacg gcattgtccg ccagctcgag
     1441 gcatccggcg agagcgaaat atcgtcgaag cagatcggcg agctggtgat ggatacgctg
     1501 aaggaagtcg atcccgtggc ctatgtgcgc tttgccagcg tctaccggaa ttttcgcgag
     1561 gcaaaggatt tcgaggactt catcggctcg ctgggtgaga ccacctagtc cagatcatgc
     1621 ccgcgccgtc gccaccctcc caagcagacg acatcgcaca catgcggtcg gctctggcgc
     1681 tggcgcgccg tggcctcggc agcacgtggc ccaacccttc cgtcggctgc gtcatcgtga
     1741 aggatggccg cgtggtgggc cgcgcggtga cagcacccgg cggccgtcct catgccgaac
     1801 cgcttgctct ggacctcgcc ggcgcatcgg cgcgcggcgc cacggtctac gtgacactcg
     1861 agccctgctg ccattggggc cgcacgccac cctgcaccga tgcactgatc agcgccggcg
     1921 tcgcccgggt ggtgatcgcc acccgcgatc cggatccgcg tgtcgacggg gccggtattg
     1981 ccaggctgcg ggccgccggc atcgtggtcg atgaaggcgt attgaatcac tcggctgacg
     2041 cgattgccgc cggcttctgg tcccgcattc aaagcgggcg cccattggtg acgctgaagc
     2101 tcgccaccac gctcgacggc cgcatcgcga cacgtaccgg cgaaagccga tggatcaccg
     2161 gcgagcctgc acgccgtgcc gcccatgcgc tgcgcggccg gagcgacgcg gtgatggtcg
     2221 gtatcggcac cgtcatggcg gacgatccgg aactcacgtg tcgggtcccc ggcttccgga
     2281 ccacgccact ggtacgtatc gtcgccgaca gccacttgcg aacacctttg accaagaagc
     2341 tgatcgccac cgcatccgaa agtccgacct ggatactgat ccgcaacggc accgatcgcg
     2401 aacgccggca cgcgttcgcc gaacttggca tcaggctcat cgaagtcacc ggtgctcaga
     2461 caggcgttga catcacccag gcactctctg ccctgggcag cgctggcctg acccgcatcc
     2521 tggtggaagg cggcgctgag ctcgcggcgt cgctgttgcg cgcggacctc gtggaccgca
     2581 tcgcctggtt ccatgccccc accgtcatgg gcggtgacgc ctggcccgcg gtgcaggcct
     2641 tcggtatcga tcacctcgcg gccatgccac gcttcactcg cgttgccgaa acagtattgg
     2701 gggacgacat gctgactgaa ttcgccagga gaacagaaca gtgagcgcct cttgcatcct
     2761 ggctccgctg gcgcagcgcg gcaaacccct ctccggccct gggggcggag ataacgggag
     2821 aggtggggga cctaactcaa tgcgtatgcc cgccgatatt ggcgcaatcc gcccctcacc
     2881 tcacctgccg gaatcgaccc caccatctac cttgtccgtc ccgccgaact gatatgttca
     2941 ccggcatcgt caccgcgctc ggcaccgtcc gcgccatcac cccactggcc accggctccg
     3001 acatgcggct caccatcgaa gccccttggc ctgacgccgc gtcgatcccc atcggtgcct
     3061 ccatcggctg ctccggctgt tgcctcaccg ccgtggaagt tggccgggac tggttcacgg
     3121 ccgatgcctc cgccgaaacc ctgtccagga ccacgcttgg ccgctggcgc cctggtacgc
     3181 gcgtcaacct ggaacgctcg ctctgcctcg gcgatgaact cggtggccat ctcgtttccg
     3241 gccatgtcga tggcgtcggc gaagccctat cctccgtgcc ggagcacggc tccgcacgct
     3301 ggaccttccg cgtcccccca gccctggccc gcttcattgc ggtcaagggc agtatcggcg
     3361 tggatggcgt atcgttgacg gtgaacgagg tatcggacga tacctttggt gtgaacatca
     3421 ttccgcatac cgcttcggtg accagctttg gctcgctccg ccccggcgat gcggtgaacc
     3481 tcgagatcga tatgctggcg cgctatgtcg cgcggctggc ggagttcaag tgaatgtcgc
     3541 agaacctgaa gaccgtcgag ctgtcacgat acctgtccgc cgccgatgag ttgatcgagg
     3601 aagcccgcaa cggccgcatg ttcatcctgg tcgatgacga ggaccgggaa aacgaaggcg
     3661 acctcatcgt tcccgcgcaa ttcgccaccc ccgacgcgat caacttcatg gcgcgccacg
     3721 cgcggggcct gatctgcctt gcactcaccc gccagcgtgt cgaacaactc ggcctgccat
     3781 tgatggccgc gcacaacggc acccgacatc agaccgcgtt caccatctcg atcgaagctc
     3841 gcgaaggcgt caccaccggc atctccgccc atgaccgcgc ccgcaccatt gccgtcgcca
     3901 tcaacccgga ggccggtcgc gacgacatcg tcacccccgg tcatgtcttc cccctgatgg
     3961 cccgcgaagg cggcacgctg gtccgcgccg ggcacaccga ggcttcggtg gatatcgccc
     4021 gcaaagccgg cctgtatccc gcggcggtga tctgcgagat catgaacgac gacggcacca
     4081 tggcgcggct gcccgacctg gtggccttcg cccagcgcca caacctgaag ctcggctcga
     4141 tcgctgacct gatcgcacac cggcggctca ccgaacggct ggtgaagcgc gttgccgaga
     4201 gcccgttcca gcacgtcgcc ggcggcgaat ggcgcgccgt cgtctacgcc aacacggtcg
     4261 aataccagga gcacctggtc ctggtgaaag gcgatgtcag cggcaacgat ccggtgatgg
     4321 tgcgcatgca cgccgtcgat ctgctggatg acatgaccgg cggcccgcac tggatcggta
     4381 tccacaatgc gatgcacatg atcagcaagg aagatcgcgg cgtcgtcgtg ctgatccggg
     4441 agcatcgcaa gaccgcactc gccgaacgcg tcggccagct cgcagccggc gatcgccggc
     4501 cacaacgaga actgcgcgac tacggcatcg gcgcacagat cctgctggat ctcggcgtga
     4561 agaacatggt gctgctgtcg aacagccggc gcaccatcat cggcctcgac ggctacggcc
     4621 tgaacatcgt ggcgcagaag ccga
//
LOCUS       DATGEM010000211         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_142932, whole genome shotgun sequence.
ACCESSION   DATGEM010000211 DATGEM010000000
VERSION     DATGEM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142932"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /gene="lnt"
                     /locus_tag="VKQ27_06675"
     CDS             complement(<1..1090)
                     /gene="lnt"
                     /locus_tag="VKQ27_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLY88652.1"
                     /translation="MFSRLLVSLAALRGFRADLAALALGALAAAALPPVHLIPVLLVS
                     VPGLLALIDGAKSPAIAARRGWWFGLGHHVLGLYWITEAILFEAARFWWLVPLAVPAI
                     AAALAVFIAVACAVARLAPVGWPRVLALAGAWVLMDLARQFVLTGFPWNLWGSDWEVP
                     GAIGDVFIQPAAYVGVHGLTLMTLVLAATPALGSRWRIGGAAFLAAWAAFGIVRLHET
                     TPVPTPLTVALLQGNVAQGQKFDQQLAVHIFRHYLDLAAQARTGVGNGALVEVWPETA
                     FPGYLDYDDTARRIIEQVTNGAPSLVGSIRFDAQRRPRNSLFALLDSGAIADMYDKWH
                     LVPFGEYIPDWLPLPIMVLPGNGFAPGPG"
     gene            1234..2205
                     /locus_tag="VKQ27_06680"
     CDS             1234..2205
                     /locus_tag="VKQ27_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY88653.1"
                     /translation="MPSLTRRALGVGALALLAAPRIARAAAPEEIHIDWATYNPVSIV
                     LKQQGLLEHEFASDKIGVRWVQSLGSNKALEFINAGSLDFSSSAGAAALIGKINGNPL
                     KSIYVYSKPEWTALVTMKDNPIHTVADLKGQRIAVTLGTDPHIFLVRALATARLTQKD
                     VKLVLLQHPDGRTALERGDVAAWAGLDPMMAAAEIESGARLFYRNADANTYGVLNVRE
                     AFAAQNPELVRRVLVTYEKARRWSVEHPADLKALMVAAMKLPDAVVARQLERNDLSSG
                     AIGDKQAATIIEAGKALQQAGIIQADVDVTAVTGKMLDPTFARAAGA"
     gene            2202..2963
                     /locus_tag="VKQ27_06685"
     CDS             2202..2963
                     /locus_tag="VKQ27_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88654.1"
                     /translation="MKRALAGLIIPILLCVAWEDAVRFGIAPGRLMPPPSQLLRTAIS
                     LIRSGDLQTHVAATMSRVGIGFGVGALAGTLFGMLTGGSPVMRRLLDPSIQALRAIPS
                     IAWVPIFILWLGIFELSKLALIALGVFFPVYLGTLSAIEDVDRKLIEVGRVFGLSRAG
                     IMLRITWPAVLPIWIAALRSGLGLGFMFVVAAELMGASEGLGYLLVYGQQMGQADVIL
                     VAMIVFALLGKLSDAVLVGVTAPLLRWQDTVRARL"
BASE COUNT          546 a          909 c         1011 g          542 t
ORIGIN      
        1 ggcccggccc cggcgcaaag ccattgccgg gcagcaccat gatcggcaat ggcaaccagt
       61 ccggtatgta ttcaccgaac ggcaccaggt gccatttgtc gtacatatcg gcgatcgcac
      121 cgctatcaag caatgcgaac aggctgttgc gggggcgtcg ctgcgcgtcg aagcggatgc
      181 tgccgacgag cgatggcgcg ccgttggtca cttgttcgat gatgcggcgc gcggtgtcat
      241 cgtagtcgag atagccgggg aatgccgtct cgggccagac ctcgaccaat gcgccattgc
      301 ccacgcctgt gcgtgcctgc gctgcgagat cgagatagtg ccggaagatg tggacggcga
      361 gctgctgatc gaatttctgg ccctgcgcga cgttgccctg gagaagcgca acggtcagcg
      421 gcgtgggaac cggtgttgtt tcatgcaggc ggacgatgcc gaatgctgcc caggcggcga
      481 gaaaggctgc gccgccgata cgccatcgcg aaccgagggc tggtgtcgct gcaagcacca
      541 gtgtcatgag cgtcagtcca tgcacgccga catacgccgc gggttgaatg aagacatcgc
      601 cgatggcgcc aggcacctcc caatcgcttc cccacaggtt ccacgggaag cctgtcagga
      661 cgaactgacg cgccagatcc atcagcaccc aggcacccgc cagtgccagc acgcgcggcc
      721 aacctacggg cgccaatcgt gcgacggcac aggcgaccgc gatgaataca gcaagcgctg
      781 cggcgatcgc cggaaccgca agcggcacca gccaccagaa gcgagcagcc tcgaacagga
      841 tcgcctcggt gatccagtaa aggccgagga cgtgatgccc aagaccgaac caccagccgc
      901 ggcgcgcggc gatcgcgggg cttttcgcgc catcgatcag cgccagcagg cctggcacgg
      961 agaccagcaa caccggaatc agatggaccg gcggcaatgc ggccgcggcg agtgcgccaa
     1021 gcgccagcgc ggcgagatcg gcgcgaaagc cgcgcagtgc cgcgaggctc acgagaagac
     1081 gagagaacat accggccggc tacacggcga ctgcttcgca ggcaacctgc atacgcgtcg
     1141 gttggtggaa agaatttcca ctcacgttcc gcgctattgc ctttattgag aaactcttct
     1201 ttgtccgcca tgcaaatggg ggaggagcca taagtgccat cactaacccg acgcgcgctg
     1261 ggtgttgggg ccctcgcgct gctcgcggcg cctcgtatcg cacgggcggc tgcgccggaa
     1321 gagatccata tcgactgggc gacctacaat cccgtcagca tcgtgctgaa gcagcaggga
     1381 ttgctcgaac acgagtttgc ttcggacaag atcggcgtcc gctgggtgca gtcgctgggg
     1441 tccaataagg cgctcgagtt catcaatgcg ggctcgctcg atttcagttc gtccgcgggg
     1501 gcggcggcgc tcatcggcaa gatcaacggc aatcccctca agtcgatcta tgtgtactcg
     1561 aagcctgagt ggaccgcgct cgttaccatg aaggacaatc cgatccacac ggtcgctgat
     1621 ctgaagggcc agcgcatcgc cgtcacgctg ggcacggatc cgcatatctt cctggtgcgg
     1681 gcactggcca ccgccaggct gacccagaag gatgtgaagc tggtcctgct gcaacacccc
     1741 gacgggcgta ctgcactcga gcggggcgat gtggcggcgt gggcagggct cgatcccatg
     1801 atggcggcgg cagagatcga gagcggcgcg cggctgttct atcgcaatgc cgatgccaat
     1861 acttatggtg tcctgaatgt ccgggaggca tttgccgcgc aaaatccaga gctggttcgc
     1921 cgtgtgctgg ttacctacga gaaggcgcga cgctggtcgg tggaacatcc ggccgacctg
     1981 aaggcgctga tggtcgccgc gatgaaactg cccgacgccg tggtcgcgcg ccagctcgag
     2041 cgaaacgacc tctcgagtgg cgcgatcggc gacaagcagg cggcgaccat catcgaggcc
     2101 gggaaggcac tgcaacaggc cggcatcatc caggcggatg tcgatgtgac ggcggtgacc
     2161 ggcaagatgc tcgatccaac gttcgcccgt gccgcggggg catgaagcgc gcgctcgccg
     2221 gactgatcat tccgatcctg ttgtgtgtgg cctgggagga cgcggtgcgg ttcggcatcg
     2281 cgcccggccg gctgatgccg cctccatcgc agctgttgcg aacggcgatc tcgctgatcc
     2341 gatcgggtga tctgcaaacg catgtcgccg cgaccatgtc acgcgtcggc atcgggtttg
     2401 gtgttggtgc gttggcgggg acgttgttcg gcatgttgac tggcggaagt cccgtgatgc
     2461 gccgcctgtt ggacccgtcg atccaggcgc tgcgtgccat tccatcgatc gcctgggtgc
     2521 cgatattcat cctctggctc ggtatcttcg aactgtcgaa gctggcgctg atcgcgcttg
     2581 gtgtgttctt cccggtctac cttggcacgc tgtcggcaat cgaggacgtg gatcgcaagc
     2641 tgatcgaggt cgggcgggtg tttggccttt cgcgcgcagg aatcatgctg cgcatcacct
     2701 ggcccgccgt tcttcctata tggattgccg cattgcggag tggcctcggt cttggcttca
     2761 tgttcgtggt cgccgcggaa ctgatgggcg cgtccgaagg tcttggctac ctcttggtgt
     2821 atgggcagca gatgggccag gctgatgtga tcctggtggc gatgatcgtg ttcgcattgc
     2881 tgggcaagct gtcggatgcc gtgctcgttg gggtgaccgc accgctgttg cgctggcagg
     2941 acaccgtgag ggcacgactg tgacgacgct cgcgatcgag gggctgggca agacctacgc
     3001 cgatggga
//
LOCUS       DATGEM010000212         6845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1416117, whole genome shotgun sequence.
ACCESSION   DATGEM010000212 DATGEM010000000
VERSION     DATGEM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416117"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VKQ27_06690"
     CDS             <1..305
                     /locus_tag="VKQ27_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88655.1"
                     /translation="RIYRSAMLTELWNTLAAGHETTMEQRARCRLAPIFAADCARDAM
                     DLMYRYGGSTSFKRGTRLAECWRDLHVVGQTQTIGPEWYPIGGRALLGMDPGPRLR"
     gene            414..2510
                     /locus_tag="VKQ27_06695"
     CDS             414..2510
                     /locus_tag="VKQ27_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLY88656.1"
                     /translation="MRPPSFLDGGGEVGALMRGHDWSSSPLGAPEAWPQSLRSVVGLL
                     LNSKFPMFVAWGPELGFLYNDAYAEILGAKHPRSLGARFYDIWSEIWPDISPLIDAAM
                     AGQASYRDDLPLVMNRKGFDEQTWFTFSYSPVRDESGAVAGMFCAVSETTQRILVERA
                     LRESEGRLRSLNETLEQRVAEALAERKVFADLFEGTDAFVQVGDMNYRWLAVNRAARD
                     EFERIFGVRPRVGGSMLDALADQPEHQAALRAIWSRALAGEEFTEVREFGDAARSRRA
                     YEIKFNTLRDRAGNQIGAFQFVTDVTERVTAQKRLAEAEEFLHQAQKIETIGQLTGGV
                     AHDFNNLLTPIVVALDQLRRRHDGSERDRRLIVGAQRAADRAGTLVQRLLAFSRRQHL
                     QPRAVDIRALVEGMADLMSRSLGPEIELVFDIADDMVPAQVDPNHLELALLNLAVNAR
                     DAMSGRGQLTITANAQTADGRQHLAPGDYIRLSVSDTGIGMDEDTLRRATEPFFTTKQ
                     VGEGTGLGLSAVQGLAEQSGGAFRLESKVGRGTIATLWLPASEQPVTEPVVDRDEVAL
                     PVLADTKSVLLVDDEELVRTGIAAMLTEVGFQVSEAGSAYEALDLLNDGLAIEVLITD
                     YAMPGMTGAELARAARSLKPDLHVLLITGYAAVSDREAGDLRRLAKPFGQARLVAAID
                     ELFKVRERAMTGHRQS"
     gene            2589..3005
                     /locus_tag="VKQ27_06700"
     CDS             2589..3005
                     /locus_tag="VKQ27_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY88657.1"
                     /translation="MAIPNRPEYQSDLFKKGLEVRRDVLGTEYVDGSISRADDFNAAF
                     QQMTTEVAWGMIWTRPGLPRKTRSMINLTMLAALNRGAEFRLHLRAALTNGVTRDEIK
                     EILLQIGAYCGIPACLEAFRIATEVFKEVDSAKGSD"
     gene            3005..3913
                     /locus_tag="VKQ27_06705"
     CDS             3005..3913
                     /locus_tag="VKQ27_06705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88658.1"
                     /translation="MADKPTVGFVGVGNMGWPMAACLVKAGFPVQVNDSRREVANNFV
                     QQIGGTAPDTLRQLAAASDVVITMLPTSVIVENVLTSGDDNVGAGMKPGTVVIEMSSG
                     VPSVTQVLAERVMELGGHMIDAPVSGGVPRAKTGELAIMVGGDSAIIDSVMPVLSAMG
                     TSVLRTGAVGSGQAMKALNNLVSAGGFLIGIEALLIGQRFGLDPAVMVDVLNAATGMN
                     NSTQKKFKQFVLSRKFNAGFTMGLLAKDLSIALQVGRETGTPSPLSALTKEMVAAAQA
                     MFGSDADHTEMAKLCERLVGMQLGKG"
     gene            3965..4225
                     /locus_tag="VKQ27_06710"
     CDS             3965..4225
                     /locus_tag="VKQ27_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88659.1"
                     /translation="MSSQGSMLGRTALVRVAVVVGVAVISFAGGAVVAAQPHMTNALH
                     ALENARGELQVAERDKGGHRVNALRLINEAISEVQAGIAAGS"
     gene            4373..5734
                     /locus_tag="VKQ27_06715"
     CDS             4373..5734
                     /locus_tag="VKQ27_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88660.1"
                     /translation="MIRNAERPALANASVESGKRWLLGTMCLAVLLAQVDTSVVNLAM
                     RAIGAGLQAGVGGMQWVLDAYNVAYAACLLTGGLLADLYGRRRLFLCGSMVFTAGSLL
                     CAAAPDIATLICGRGIAGVGAALLLPASLAIIRVEWRDAQARNHALGIWAACNGLAFV
                     IGPTVGGALVDRFGWRSVFLVVVPLGVAAFGLALRVVPESRDRLGRQFDMTGQVVGAA
                     MLGCLTVAGIEAAHIQEVALAALVTAVAALLLFLAMERRRGDAAMVPLDMFRSRRFNG
                     ALAATGGMTFGMYGALFLIPLVWQRSGTFSAVSAGIALLPMAGVFFVVSNASGRLVDH
                     LGSRVLIGGGTALTGLGLAVLACTTAGSPLCLAEVGLVLTGLGMGCNTAPLYGVVVAA
                     VPAERSGSAASLINVARMVGATLGVAVLGSIFAAVGLREAMLVGGAVQIVGAGAAWLA
                     LAE"
     gene            complement(5805..6620)
                     /locus_tag="VKQ27_06720"
     CDS             complement(5805..6620)
                     /locus_tag="VKQ27_06720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLY88661.1"
                     /translation="MRGSVGKKIDAGASADIHIWAPGQVVKLFKPGGSQWFARHEARM
                     TRAALAAGAPEVFDEVTVGGRFGIVLRHLAGPTLQQLLLTGAMTPGQAGAILASLYIS
                     IHNTPPSSDVPSLRRWIDFASQASPDVLPRHIATGVLSLIDRLPPGNGLCHSDLHPGN
                     VIMTTDGPRIIDWACALRASGVFDIARVHVSLSELLPDNVDPEPPRVINAIVQSEYAR
                     LSGSSPAALTEAMQPYLPILRIFVLLQRRRATPAQRDLLIQRVEAALRSQGLA"
BASE COUNT         1178 a         1944 c         2457 g         1266 t
ORIGIN      
        1 ggcgcatcta tcgcagcgcg atgctcaccg agttgtggaa cacgcttgcc gcagggcacg
       61 agacgacgat ggagcagcgc gcgcgctgcc ggctggcacc gatctttgcc gccgactgcg
      121 cgcgcgatgc gatggacctg atgtatcgct atggtggcag cacgtcgttc aagcggggga
      181 cacggctggc ggagtgctgg cgcgatctgc atgtcgtggg ccagacgcag acgatcgggc
      241 ctgaatggta tccgatcggc gggcgggcat tgctgggcat ggacccgggg ccgcgcctgc
      301 gataggactg ttctgcctgg gtcacttttg cgtaagagca attacagatt gggtgcatcc
      361 gcgcgcttct gctaagctga acccatgcgt cggaccgtac agcggacaag tgggtgagac
      421 cgccgagctt tcttgatggc gggggcgaag tcggtgcact gatgcgagga catgactggt
      481 cgtcctcgcc gttgggtgcc ccagaagcgt ggccgcagtc actgcgctct gtcgttggcc
      541 tgctgctgaa ctccaaattc ccgatgttcg tcgcgtgggg cccggaactc gggttcctgt
      601 acaacgacgc atacgccgag atccttggcg ccaagcaccc gcggtcgctg ggtgcaaggt
      661 tctacgatat ctggtcggag atctggcccg acatcagtcc gctgatcgac gcggcgatgg
      721 cagggcaggc aagctatcgc gacgatctgc cactggtgat gaatcgcaag ggcttcgacg
      781 aacagacgtg gttcacgttc tcctattctc ccgtgcgcga tgagagtggc gccgtcgccg
      841 gaatgttctg cgcggtttcc gagacgacgc agcgcatact tgtcgagcgg gctttgcgcg
      901 agagcgaagg gcgactgcgt tcgctgaacg agactttgga acagcgcgtt gcggaagcgc
      961 tcgcagaacg aaaggttttc gctgacttgt ttgaaggcac ggatgcgttc gtgcaagtcg
     1021 gcgacatgaa ctatcgttgg ttggccgtca atcgcgcagc acgcgatgag ttcgagcgca
     1081 tatttggtgt gcggccgcgg gtgggcggca gcatgctcga tgcgttggcg gaccagccgg
     1141 aacaccaggc cgcgctgcgg gcaatatgga gtcgtgccct ggctggtgaa gagttcaccg
     1201 aagtgcgtga attcggcgat gccgcgcggt cacgccgggc ttatgagatc aagttcaaca
     1261 cgctgcggga ccgcgcaggc aatcagattg gcgcattcca gttcgtcacc gacgtgacgg
     1321 agcgggtgac ggcgcaaaag cgtctcgccg aagccgagga atttctgcat caggcgcaga
     1381 agatagagac catcggccaa ttgaccggcg gcgtggcgca cgacttcaac aatctgctga
     1441 ccccgatcgt tgtcgcgctt gaccaactgc ggcggcgtca tgatggcagc gaacgggacc
     1501 gccggctaat cgttggtgcc cagcgggcgg ccgacagggc agggacgctc gtgcagcggc
     1561 tgctcgcatt ctctcgccgg cagcatctgc agcctcgcgc ggtggatatc cgggcactgg
     1621 tcgaaggcat ggctgatctg atgtcacggt ccctgggccc cgagatcgaa ctcgtgttcg
     1681 acatagctga cgatatggtg cctgcgcagg ttgacccgaa tcaccttgaa ctcgcgctgc
     1741 tcaatctggc cgtgaatgcg cgggatgcca tgtcggggcg ggggcaactg acgatcacgg
     1801 cgaatgccca gacggcggac ggccggcagc atctggctcc gggggactat atccgactgt
     1861 cagtgtccga caccggcatc ggcatggacg aggatacatt gcggcgcgca accgagccgt
     1921 ttttcaccac aaagcaggtt ggtgaaggca ctggtctagg gctatcggcg gttcaaggcc
     1981 ttgcggaaca gtcgggtggc gcgttccgcc tggagagcaa ggtgggacgc ggcaccattg
     2041 cgacgctttg gctgccggcg tcggagcagc cggtcacgga gcctgtcgtt gaccgcgacg
     2101 aagtggcatt gcccgtgttg gccgatacga agtcggttct acttgtcgat gatgaggaac
     2161 tcgtgcggac gggtatagcc gccatgctga cggaagtcgg atttcaggtc agtgaagccg
     2221 gctctgccta tgaagcgctg gacctgttga atgatgggct ggctatcgaa gtgctgatca
     2281 ccgactacgc catgcctggc atgaccggcg cggaactggc gcgcgcggcg cgttcgctga
     2341 agccggatct gcatgtgctg cttatcactg gctacgccgc agtgagcgat cgcgaggccg
     2401 gtgatttgag acgacttgcg aaaccattcg ggcaggctcg tctcgtcgcg gcaatcgacg
     2461 agcttttcaa agtgcgcgaa cgcgcgatga cgggacatcg gcagagctga cgcttctgaa
     2521 tgccgtccgc ttgccgggaa aagtcgcact ctctacggtc cgcccctggc aaacgaagga
     2581 gcaccgcgat ggccattccc aatcgtcctg agtaccagag cgacctgttc aagaagggac
     2641 tggaggtacg ccgcgacgtg ctcggcaccg agtatgtcga tggctcgatc agccgcgcgg
     2701 atgatttcaa cgcggcgttc cagcagatga ccaccgaggt cgcatggggc atgatctgga
     2761 cgcggccagg gctgccgcgc aagacgcgca gcatgatcaa cctgacgatg cttgcggcgt
     2821 tgaatcgcgg cgcggagttc cggctgcatc tgcgcgcggc gctcaccaac ggcgtgacgc
     2881 gcgacgagat caaggagatc ctgctgcaga tcggcgcgta ttgcggcatt ccggcctgcc
     2941 ttgaagcgtt ccgcatcgcg accgaggtgt tcaaagaggt cgacagcgcg aaaggcagcg
     3001 actgatggcc gataagccaa ccgtcggctt cgtcggcgtc ggcaacatgg gctggccgat
     3061 ggcggcctgt ctggtgaagg ctggcttccc tgtgcaggtg aatgattcac ggcgcgaggt
     3121 ggcgaacaat ttcgtgcagc agatcggcgg cacggcgccg gacacgctgc gtcagctcgc
     3181 cgcggcgtcg gatgtggtga ttaccatgct gccgacgagc gtgatcgtcg agaacgtgct
     3241 gaccagcggc gacgataacg tcggtgccgg catgaaaccc ggcaccgtgg tgatcgagat
     3301 gagctccggt gtgccatcgg tcacgcaggt gctggccgag cgcgtgatgg aactcggcgg
     3361 tcacatgatc gacgcgccgg tgtctggcgg tgtgccccgc gccaagacag gcgagcttgc
     3421 gatcatggtg ggcggtgaca gcgcgatcat cgactcggtg atgccggtgc tgagcgcgat
     3481 gggcacgtcg gtgctgcgta ccggcgcggt tggatccggt caggcgatga aggcgctgaa
     3541 caatcttgtc agcgccggcg gcttccttat cggcatcgag gcactgctga tcggccagcg
     3601 cttcgggctg gatcccgcag tgatggttga cgtgctgaat gccgcgacgg gcatgaacaa
     3661 ctcgacccag aagaagttca agcagttcgt gctgtcacgg aagttcaacg ccggcttcac
     3721 catggggctg ctggcgaagg acctgtcgat cgcgcttcaa gtggggcgcg aaaccgggac
     3781 accctcgccg ttgtcggcgt tgaccaagga gatggtggcg gcggcgcagg cgatgttcgg
     3841 atcggacgcc gatcacacgg agatggctaa actttgtgag aggctggtcg gcatgcagct
     3901 tggaaagggc tgacaaatcg attgccgata cggcatgatc cggcgttcct ttcaggaggg
     3961 aaacgtgagc agtcaaggtt ccatgcttgg ccgtaccgct ttggtgcgtg tcgctgtggt
     4021 cgtcggtgtt gccgtgatca gtttcgccgg cggcgccgtc gtggcggcgc agccgcacat
     4081 gacgaatgcg ctgcacgctc tggaaaatgc cagaggtgaa ttgcaggttg ccgaacgtga
     4141 caagggtggt caccgggtca atgcgctgcg cctgatcaac gaggcgatca gcgaagtgca
     4201 ggcgggcatc gccgccggat cgtgatacgc ggctgccgct tcgaacggag cggcggcacc
     4261 gaggtctcgt ccgacgtcct attgtgaagg ctaccggcaa tggctgctga cagcacgatg
     4321 tcagcagcca ttgcctatgt cggtggcgag tttccacgac aggcgatcgg acatgatacg
     4381 aaatgcagag cgaccggcgc tggcgaatgc cagcgtcgaa agcggcaagc ggtggctgct
     4441 gggcacgatg tgcctggccg tgctgttggc gcaggtcgat acatcggtgg tcaacctggc
     4501 gatgcgcgcg atcggcgccg gcctgcaggc cggcgttggc ggcatgcaat gggtgctcga
     4561 cgcatacaac gtcgcgtacg cggcgtgcct tctgacgggg ggcctgctgg ctgacttgta
     4621 cggcaggcgg cggctgttcc tgtgtggctc gatggtgttc acagccggct cgctgctgtg
     4681 cgctgcggcg ccggacatcg cgacgctgat ctgcgggcgc ggcatcgccg gcgtgggcgc
     4741 ggcactgctg ctgccggcat cgctggcgat catccgcgtc gaatggcgcg acgcgcaggc
     4801 tcgcaatcac gcgcttggca tctgggctgc gtgcaacggg ctggcattcg tcattggccc
     4861 gacggttggc ggagcattgg tggatcgctt cggctggcga agcgtgttcc tggtggtggt
     4921 gccgctgggc gtggcggcgt tcggcctcgc actgcgtgtc gtgcctgagt cacgcgaccg
     4981 gctggggcgg cagttcgata tgaccggaca ggtggtgggt gccgcgatgc tgggctgtct
     5041 gacggtcgcg ggcatcgagg cggcgcatat acaggaggtg gcgcttgctg ccttggtcac
     5101 ggccgtggcc gcactgctgc tgttcctggc aatggagcgc cggcgcggcg acgcggcgat
     5161 ggtgccgctg gacatgttcc gcagccgccg gttcaacggc gctctggccg ccaccggagg
     5221 gatgaccttc ggcatgtatg gcgcgctgtt cctgatcccg ttggtctggc agcgctcggg
     5281 cacattcagc gcggtcagcg ccggcatcgc gctgctgccg atggctggcg tgttctttgt
     5341 tgtctcgaac gcatcggggc ggctggtcga tcacctgggt tcgcgggtgc tgatcggggg
     5401 cggcacagca ttgaccggcc tcggccttgc tgtcctggcg tgcacgacgg cgggctcgcc
     5461 gctgtgtctt gctgaagtcg gactggtgct gaccgggctc gggatgggct gcaacaccgc
     5521 acctctgtat ggcgtggtgg tcgccgcagt tcccgcggag cgatcaggca gcgcggcgtc
     5581 gctgatcaat gtcgcgcgga tggtgggagc gacgctgggc gttgcggtgc tgggatcgat
     5641 cttcgcagcc gtggggctgc gcgaggcaat gctggtgggc ggtgcggtgc agatcgttgg
     5701 cgccggggcg gcgtggctgg cactggcgga atgattcaac gcacagtacg cgaagggacg
     5761 cgaaggtcag ggaggcttgt ttggcgtcgt gcctttgggt tcgatcaggc caagccctgc
     5821 gagcgcaggg ctgcttcgac gcgctggatc agcaggtccc gttgggcagg tgtggctcgc
     5881 cgccgctgaa ggagcacgaa gatgcggaga atgggcagat atggctgcat cgcctcggtc
     5941 aacgccgctg gggacgaccc agagagccgc gcgtactcgg actgcacgat ggcattgatc
     6001 acgcgcggcg gttccggatc gacgttgtcc ggtaagagct cggaaaggct gacgtggacg
     6061 cgtgcgatgt cgaagacgcc ggatgcgcgg agcgcgcagg cccagtcgat gatgcgcggg
     6121 ccatccgtcg tcatgatcac gttgccgggg tggagatcgg aatgacacag cccattccct
     6181 ggcggcagac gatcaatcag gctcaggaca ccggtggcga tgtgccgcgg cagtacgtcg
     6241 ggggaggcct gggacgcgaa atcgatccaa cggcggagcg aggggacatc cgacgatggc
     6301 ggcgtattgt gaatggatat gtagaggctc gcgaggatgg cgcctgcctg cccgggcgtc
     6361 atggcgccgg tcagcaggag ttgctgcagg gttggtccgg caagatgacg cagcacgatg
     6421 ccaaagcgcc caccgacagt cacttcgtcg aacacctctg gagcgcctgc ggccagcgcg
     6481 gcgcgggtca tgcgtgcctc gtgccgcgcg aaccattgcg agccgccggg cttgaacagt
     6541 tttaccacct ggccaggtgc ccagatatgg atgtcggccg aggcgcctgc gtcgatcttt
     6601 ttccccacgg acccgcgcat cgcccgtcct accgacccct gtgcctctta gtccagtcga
     6661 gatgcatcag gcgaaggcca gttgttgagc gcggcggcgc gagaccagga agctgatgac
     6721 gatgaggttg gcgatgttga agaatacgcc gacgccgaag gctggcgcgt agaagccgaa
     6781 gtgatcgtaa agggcgccgg cgagccagct gccgaatgcc atgcctgaca tgccgaggaa
     6841 gaaga
//
LOCUS       DATGEM010000213         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1191353, whole genome shotgun sequence.
ACCESSION   DATGEM010000213 DATGEM010000000
VERSION     DATGEM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191353"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VKQ27_06725"
     CDS             complement(<1..222)
                     /locus_tag="VKQ27_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88662.1"
                     /translation="MIEAGTSRPAAVFGRPTPAPKPVFDAGAGGSGEGRPAWLERETT
                     LERSVKIGVFVVVVAAVLYPFLSVLATSLA"
     gene            complement(219..1082)
                     /locus_tag="VKQ27_06730"
     CDS             complement(219..1082)
                     /locus_tag="VKQ27_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY88663.1"
                     /translation="MLLFVLPGVLYFVLFGYLPLLGNIIAFEDYLPFVGFGSPFVGFD
                     NFAAMISDTAFWQALSNTIIITLLQLVFYFPAPIILALLMQSIISTKIRRIIQSVVYL
                     PHFISWVIVVSLWQQVLGGSGIFVHTLRDLGLPAINVMTEPGLFKALATSQLIWKETG
                     YATIIFLAALLNIEAALYEAAVMDGAGRWQRLWNITLPGIMGVVILLLILRLGLTLSV
                     GFEQILLQRNAVGPAAGEVLDTYVYFKGIVGGQWGITAAAGLIKGIFGTAIVIAANKL
                     AHRFGQEGVYS"
     gene            complement(1338..>2121)
                     /locus_tag="VKQ27_06735"
     CDS             complement(1338..>2121)
                     /locus_tag="VKQ27_06735"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HLY88664.1"
                     /translation="EPGEIDPHRVGLAACFVARVVKRTVAPDFGGLARYRRAPDVPRT
                     YLGRPAWTRAQMAERAASLLPEHSYVNLGLGIPTLVSNYLSGRDITLHGENGILGYGR
                     TADRDHVDHYVHDAGGNYVTEQPGVAYFDSVTAFEMVRSGKIDVVVLGAYQVDEAGNV
                     ANWSTPEQVGGGIGGAMDMVAGGGTVMILMEHRDSRDRAKLVRSCTYPLTGAGCVDLV
                     VTDLAVLRRVDGHFVVEEIASGFSTAEIKSLTEMDILSKTGA"
BASE COUNT          408 a          668 c          693 g          352 t
ORIGIN      
        1 ggcaagactg gtggcgagca cgctcaggaa cgggtacagc acggccgcga ccacgaccac
       61 gaacacgccg atcttgaccg aacgctcgag ggtcgtctca cgttcgagcc acgctggccg
      121 accctcgcca ctgccgcccg cgcccgcgtc gaagaccggc ttgggcgccg gcgtcgggcg
      181 gccaaagacc gccgctgggc gcgaggttcc cgcttcgatc acgagtagac cccttcctga
      241 ccgaagcgat gggcgagttt attggccgcg atgacgatcg cggtgccaaa gatacccttg
      301 atcaacccgg ccgcggccgt gatcccccac tgcccgccga cgatgccctt gaagtacacg
      361 tacgtgtcga gcacctcacc cgctgccgga ccgacggcgt tgcgctgaag caggatctgt
      421 tcgaagccga ccgatagcgt caggcccagc cgcaggatga gcagcagaat caccacgccc
      481 atgatgcccg gcagggtgat gttccacagc cgttgccagc gtccggcgcc gtccatcacg
      541 gccgcttcgt agagggctgc ttcgatgttg agcagcgcgg ccaggaagat gatcgtggcg
      601 tagccggtct ctttccagat cagctgcgac gtcgcgaggg ccttgaacag gcctggctca
      661 gtcatcacgt tgatggccgg caagccgagg tcgcgcaggg tgtggacgaa gatccccgag
      721 ccgccgagca cctgttgcca gagcgagacg acgatgaccc acgagatgaa gtggggcagg
      781 tagacgacgc tctgaatgat ccgtcggatc ttggtgctga tgatgctctg cattagcagc
      841 gccaggatga tcggagccgg gaagtagaag acgagctgga gcagggtgat gatgatcgtg
      901 ttcgacaggg cttgccagaa ggccgtgtcg ctgatcatcg cggcgaagtt gtcgaagccg
      961 acgaacggac tgccgaagcc aacgaagggc agatagtcct cgaaggcgat gatgttgccg
     1021 agcaacggca ggtagccgaa caggacgaag tacagcacgc ccggcaggac gaagagcagc
     1081 atcagtcggt cgcgccagat tcgcgtccaa acgctcacgc gcggaggcgt ggctgtctgt
     1141 gcgcgaaccc cgcctagcgt tccggctaca cgattgacgt caacggcact catgtcgcga
     1201 tctccgatcg acgcacctga cctgcggaat ggtcattcag tccgcgaccc ctgatcaggg
     1261 agcgtgcgct gcgagggttt tgtccgccaa ggaattaggt acgagcatac cctcctcggg
     1321 ctgacgagaa aagatagtca ggcgcctgtc tttgacagta tgtccatctc tgtcaaggac
     1381 ttgatttcgg ctgtcgaaaa ccccgaggcg atttcctcca cgacaaagtg gccgtccacc
     1441 cgacgcagta cggcgagatc ggtgacgacc aggtcgacgc agccggcacc ggtgagcgga
     1501 tacgtgcacg atcgcaccag tttggcacga tcgcggctgt cacggtgctc catcaggatc
     1561 atcaccgtgc cgccgccggc gaccatgtcc atcgcgccgc caatgccgcc gccaacctgc
     1621 tcgggcgtgc tccagttggc aacgttgccg gcctcgtcaa cctggtacgc gcccagcacg
     1681 acgacgtcga tttttcccga gcgcaccatc tcgaacgcgg tcacgctgtc gaagtaggcc
     1741 acgccaggtt gttcggtgac gtagtttccg cccgcgtcgt gcacgtagtg gtcgacgtgg
     1801 tcccggtctg ccgtacgacc gtagccgagg atgccatttt cgccgtgcag ggtgatgtca
     1861 cgcccggaga ggtagttgga caccagggtc gggatgccga gcccgaggtt cacgtagctg
     1921 tgttcgggca gcagcgacgc ggcacgttcg gccatctgcg cccgcgtcca ggcaggccgg
     1981 ccaaggtatg tgcgcggaac gtcgggtgcg cgacggtagc gggcgaggcc gccgaaatcg
     2041 ggggccaccg tccgcttgac gacgcgtgcc acaaaacagg ccgccaggcc gacgcggtgc
     2101 ggatcgatct cacctggttc g
//
LOCUS       DATGEM010000214         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1571570, whole genome shotgun sequence.
ACCESSION   DATGEM010000214 DATGEM010000000
VERSION     DATGEM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571570"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /gene="hisD"
                     /locus_tag="VKQ27_06740"
     CDS             complement(<1..1245)
                     /gene="hisD"
                     /locus_tag="VKQ27_06740"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLY88665.1"
                     /translation="MTRLDTSNARFEHDFAALLNQTRETTESVDQAVAAIIADVRGEG
                     DAALLRYTYRFDRVPLTADRLRIRADEIDAAVAAVSQELMAALDLAATRIEAFHRLQM
                     PSDMQTTDAAGLTLGMRWLPLDAVGLYVPGGKAAYPSSVLMNALPARVAGVSRIAMCV
                     PSPDGVLNPLVLAAARRAGVSEIYRVGGAQAVAALAYGTASIPPVDRIVGPGNAYVAE
                     AKRQVFGRVGIDAIAGPSEVVVLADAENEPRRVAVDLLAQAEHDEAAQAILITDDPAF
                     ADRIAAAVSEELASLPRSAIAGASWQQHGAIIVVRDWDEAVGLVNRLAPEHLELMLRD
                     PTAVFAKVRHAGAVFLGAFCPEAVGDYVAGPNHVLPTGRTARFASGLSVFDFLKRTTW
                     VEADAASLQRIGPAAVSLAKAEG"
     gene            complement(1304..1996)
                     /gene="hisG"
                     /locus_tag="VKQ27_06745"
     CDS             complement(1304..1996)
                     /gene="hisG"
                     /locus_tag="VKQ27_06745"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLY88666.1"
                     /translation="MTAPYPTTALEPVATTPLIMALPKGRILSECGALLARAGIVPAQ
                     DYADEESRRLRFPTDDATLDVVRVRPFDVATFVAFGAAQIGICGADVLMEFDYPEIYA
                     PLDLGIGACRVSVAEPAETAGTDDPSRWSRVKVATKYPNIARRHFASRGVHADVVHLN
                     GAMELAPGLGLSRLIVDLVQTGSTLRANGLVETEVIAQVTSRLIVNRTSLKTQPELIG
                     RWIARFRAALEQ"
     gene            complement(2087..2263)
                     /locus_tag="VKQ27_06750"
     CDS             complement(2087..2263)
                     /locus_tag="VKQ27_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88667.1"
                     /translation="MRISFLLPLVVLGLAGCVDVHEHPNPQPTATVVTPAPAPTATYV
                     APAPPTTTTVVRTP"
     gene            2420..4054
                     /locus_tag="VKQ27_06755"
     CDS             2420..4054
                     /locus_tag="VKQ27_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HLY88668.1"
                     /translation="MWTLLDLAGAVALLLWGLHMVQTGIQRGFAANLRRWLGTALGNR
                     LAAFAAGLGVTAILQSSTATGLMVAGFAAGGAVALVPALAVMLGANVGTTLIVQLLSF
                     DVAQVAPVLTLLGVIMFRRGTAARSRDLGRVAIGLGLMLLSLHQLVSLISPYEDLPNL
                     RMVLGAITTTPVLDVLLAAGLTWAAHSSVAVVLLVMSLASKGTVPPDAAFALVLGANL
                     GTAINPVLEGVSGDDPVARRLPIGNLLTRVAGCLIALPLLDRIGAVVVQLEPDPARAV
                     ADFHTGFNLILALFFLPALNPYAWALRKLLPARIDVDDPSRPRYLDQTALESPPIALA
                     AAAREALRMADALEAMLTRAGEMLAITDHRQTGDVRRLDDVLDRLNTAIKKYLANLDP
                     ESLSDDDQRRLTQILVFATNLEAAGDVVDRDVMGHIAKCLKRGRPLTSDDCAEGRQLL
                     NRLGATVRAAAAVFMSEDARAARQLAAEKEAFRDLEAAATRAQFAGLRQGQKTPLDVD
                     LVRELKRVNGHLVAAAAYPVLEGQGELLSSRIRLDS"
     gene            4274..>5729
                     /locus_tag="VKQ27_06760"
     CDS             4274..>5729
                     /locus_tag="VKQ27_06760"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HLY88669.1"
                     /translation="MPGRHQVHIDRSRDVLLTDFGRATLSDRYLMPGEEFQDLFARVA
                     SYYGDDTAHAQRIYDYMSKLWFMPATPVLSNGGTQRGLPISCFLNEASDSLEGIVGLW
                     NENVWLASKGGGIGSYWGNLRSIGEKVGAVGKTSGVIPFIRVMDSLTLAISQGSLRRG
                     SAAVYLPVSHPEIEEFIEIRRPTGGDPNRKALNLHHGILVSDAFMRAVEADEPWPLLS
                     PKDESVVRSISARSLWIRILTARIESGEPYLIFSDHVNSARPEHHKLAGLEVKTSNLC
                     SEITLPTGRDQHGMERTAVCCLSSLNLENWFEWKDHPTFIEDVMRFLDNVLQDFIDRA
                     PPGMEKAKYSAARERSVGLGVMGFHSFLQAQNVPWESVVAKVWNRRMFKHIREQADIA
                     SRVLAEERGPCPDAAEYGIMERFSHKMSIAPTASISIIAGNSSPGIEPIAANVFLQKT
                     LSGSFTVRNRHLQQLLEARGMDTDEVWSSITLRSG"
BASE COUNT          941 a         2018 c         1759 g         1011 t
ORIGIN      
        1 gccctcggcc ttggccaagg acactgcggc gggaccgata cgctgcaagg aggcggcgtc
       61 tgcctccacc cacgtggtcc gcttgaggaa atcgaatacg gacagccccg aggcaaaccg
      121 cgccgtgcgt cccgtcggca gcacgtggtt cggtcccgct acgtaatcac ccactgcttc
      181 cgggcagaat gcgccgagga acaccgctcc ggcatgccgc accttggcaa acaccgcggt
      241 gggatcgcgc agcatcaact ccagatgttc gggcgccagg cgattcacca gcccaacggc
      301 ttcatcccag tcgcgcacca cgatgatcgc gccatgctgt tgccaactcg caccggcaat
      361 cgctgagcgc ggcaggctcg ccagctcttc gcttactgcc gccgcgatgc gatcggcgaa
      421 ggcaggatca tcggtgatca gtatcgcctg ggcggcttca tcatgttctg cctgtgccag
      481 cagatcgacg gcaacgcgcc ggggctcgtt ctcggcatcg gccagcacga cgacttccga
      541 cggcccggcg atcgcatcga tgccgacgcg cccgaacacc tgccgcttgg cttccgccac
      601 gtaggcgttg cccggaccga cgatccgatc caccggtggg atggatgctg tgccatacgc
      661 cagcgccgcg accgcctgcg cgccgccgac gcgatagatc tccgacacgc ctgcgcgtcg
      721 ggcggccgcc agcaccagcg ggttcagcac gccatctggc gacggcacgc acatcgcgat
      781 ccgtgacacg ccggccaccc gcgcgggcaa ggcgttcatc agcaccgagg acggatacgc
      841 cgcctttccc cccggcacgt agagcccgac tgcgtccagc ggcagccagc gcattcctag
      901 cgtgagccct gccgcatccg tcgtctgcat atcgctgggc atctgcaacc gatgaaatgc
      961 ttcgatgcgc gtcgccgcca gatccagcgc cgccatcagc tcctgcgata ccgcggcgac
     1021 tgcggcatcg atctcatcgg cacggatacg cagccgatca gcggtcaatg gcacccgatc
     1081 gaaccgatac gtatagcgca acagcgccgc atcaccttcg ccgcgcacat cggcgatgat
     1141 tgccgcgaca gcctgatcga cgctctccgt cgtttcacgc gtctggttca gcagcgcggc
     1201 aaagtcgtgc tcgaacctgg cattcgatgt gtcgaggcga gtcatgggtg gctccgggcg
     1261 atcgcccggc ttttgcaggt catcgccggg gctgacccgg ccatcactgt tcgagcgcgg
     1321 cgcggaatcg cgcgatccac ctgccgatga gctccggctg ggttttcagc gaggtgcgat
     1381 tcacgatcag ccgcgaggtc acttgcgcga tcacctctgt ttccaccagc ccattggcgc
     1441 gcagtgtcga tccggtctgc accagatcga cgatcagccg ggacaggccc aagcctggcg
     1501 ccaactccat cgcgccgttc aagtgcacca cgtctgcatg cacgccacgt gatgcgaaat
     1561 gccgtcgcgc gatgtttggg tacttggttg cgaccttgac ccgcgaccac cgcgacggat
     1621 catcggtccc ggccgtttcg gccggttccg ccaccgacac ccgacacgcg ccgatcccca
     1681 gatccaaagg tgcatagatt tccggatagt cgaattccat cagcacatcg gcgccgcaga
     1741 ttccgatctg tgccgcaccg aacgccacga aggtggcgac gtcgaacggc cggacgcgca
     1801 ccacatccag cgtcgcatcg tcggtgggga accgcagccg gcgactttcc tcgtcggcgt
     1861 agtcctgtgc gggcacgatg cccgctcgcg ccaggagcgc gccgcactcc gacaggatac
     1921 gccccttggg tagcgccatg atcagcggcg tcgtcgccac gggttccagt gctgtggtgg
     1981 gatagggggc ggtcatggtg cgcgggcgtc tacaccacac cggcggaaaa aacgagggcc
     2041 gggactgggc ccggccctcg cttcatgaag ctgtcaactc agacgatcag ggcgtgcgca
     2101 cgaccgtcgt ggtggtgggc ggcgcgggcg ccacataggt tgcggtcggt gccggagccg
     2161 gggtgaccac ggtcgccgtc ggctgcgggt tcgggtgctc gtgcacgtcg acgcacccgg
     2221 caaggcccag cacaaccaac ggcagcagga aggaaattcg catgtcagac gtatccttca
     2281 gttgcgggat agggacatgc atgaatctgc tgcgttccgt ggccggtccg tgccggattt
     2341 gtggctgttt cctggccaac cgttcacgag gcgttgctga tcgttgaccc caggcagcaa
     2401 tcaccgaaca ctgaacgcca tgtggaccct gctcgacctc gccggcgcgg tggctctgct
     2461 gctgtgggga ctgcacatgg tgcagaccgg cattcagcgt ggcttcgccg cgaacctccg
     2521 ccggtggctc ggcaccgcgc ttggcaaccg gctggctgcg ttcgcggccg gcctgggcgt
     2581 caccgccatc ctgcaaagca gcactgccac cggcctgatg gtcgccggct tcgccgccgg
     2641 cggcgctgtc gccctggtcc ccgcgcttgc cgtcatgctg ggcgccaatg tcgggaccac
     2701 gctgatcgtg caactgctct cgttcgatgt ggcccaggtc gcgccggtcc tgaccctgct
     2761 gggcgtcatc atgttccgcc gcggcaccgc tgcccgctca cgcgatcttg gccgcgtcgc
     2821 catcggcctc ggattgatgc tgctgtcgct gcatcagctc gtctcgctga tctcgcccta
     2881 cgaggacctg ccgaacctcc gcatggtcct aggcgcgatc acgaccacgc cggtgctcga
     2941 cgttctgctg gccgcgggcc ttacctgggc ggcgcactcc tcggtcgccg tcgtcctgct
     3001 ggtgatgtcg ctcgcctcca aagggacagt cccgcccgac gccgcgttcg ccctggtgct
     3061 gggcgccaat ctgggcaccg ccatcaatcc cgtcctggag ggcgtttcgg gcgatgatcc
     3121 tgttgcgcgg cgccttccca tcggcaacct gctcacccgc gtcgccggct gcctcattgc
     3181 ccttcctctg ctcgacagga tcggcgcggt ggtggtccag ctcgagcccg atcccgcccg
     3241 cgcggtcgcc gacttccaca ccggcttcaa cctgatcctg gcccttttct tcctgccggc
     3301 gctcaaccct tacgcctggg cgctgcgcaa gcttctgccc gcccgcatcg atgtggacga
     3361 cccgtcacgc ccccgctacc tcgaccagac tgcactcgaa tcgccaccca tcgcccttgc
     3421 cgcggcggcc cgcgaggcgc tccgcatggc cgatgccctg gaagccatgc tcacccgcgc
     3481 cggcgagatg ctcgccatca cggaccaccg tcagacgggc gatgtccgcc gcctcgacga
     3541 cgtgctcgat cggctgaaca cggccatcaa gaaatacctt gcgaacctcg accccgagtc
     3601 gctctcggac gacgatcagc gccggctgac gcaaatcctg gtttttgcga ccaacctgga
     3661 ggctgccggg gatgtggtgg atcgcgacgt gatgggccac atcgccaagt gtctgaagcg
     3721 cggccgcccg ctcacttcgg acgattgcgc cgaaggccgt cagctgctga accgtctggg
     3781 cgccaccgtc cgcgccgctg ccgccgtgtt catgagcgag gacgcccgcg cggcccgcca
     3841 gcttgcagcc gagaaggaag cattccgcga cctggaggcc gcagccaccc gcgcgcagtt
     3901 cgctggtctc cgccagggtc agaaaactcc cctggacgtc gatctcgtcc gcgaactgaa
     3961 acgagtgaac ggacaccttg tagccgccgc tgcctacccc gttctggagg gccagggcga
     4021 attgctgtcg tcgcggattc ggctggattc ctgacccgcg cagtcacaca taatcgaatc
     4081 ccccgtctct cccgtcccgc cggctgagtc ggttgtctat acagcacact gtgtccagat
     4141 cgcaacacgc gccatgtatg ttgtggctgg gtcagaaaag gtgtcccaga tgttgattga
     4201 cgacacacgc gtgacgggtc gagatttgcc tccaattcgc cagggggaat ccgacgtgct
     4261 cgacgccgtg cagatgccag gtcgccacca ggtccacatc gatcgctccc gtgacgtgct
     4321 gctcaccgat ttcgggcgcg ccacgctctc tgaccgttat ctgatgcctg gcgaggaatt
     4381 ccaggacctt ttcgcccgtg tcgcctccta ttacggcgac gacaccgcgc acgcccagcg
     4441 catctatgac tacatgtcca agctgtggtt catgcccgcc acgcccgtgc tctccaacgg
     4501 cggaacccag cgcggcctgc ccatttcctg cttcctgaac gaggcctccg acagcctgga
     4561 aggcatcgtc gggctgtgga acgagaacgt ctggctcgct tcgaagggcg gcggcatcgg
     4621 ctcttactgg ggcaatctgc gctccatcgg cgagaaggtt ggcgcggtcg gcaagacctc
     4681 cggcgtgatc ccgttcatcc gcgtgatgga ttcgctgacg ctggccattt cccagggctc
     4741 gctccgccgc ggttcggctg ccgtctacct gccggtctca catcccgaga ttgaggaatt
     4801 catcgagatc cgccgcccca ccggcggcga cccgaatcgg aaggcgctca acctccacca
     4861 cggcatcctc gtttccgacg ccttcatgcg tgccgtcgag gccgacgaac cctggccgct
     4921 gctctccccc aaggacgaat cggtggtgcg cagcatctcg gcccgctcgc tgtggatccg
     4981 tatccttacc gcgcgcatcg aatcgggtga gccgtacctg atcttctccg accacgtgaa
     5041 cagcgcccgc cccgagcatc acaagctcgc cggcctggaa gtgaagacct ccaacctgtg
     5101 ctccgagatc acgctgccca ccggccgtga ccagcacggc atggagcgca ctgcggtctg
     5161 ctgcctgtcc tcgctgaacc tggagaactg gttcgagtgg aaagaccatc cgaccttcat
     5221 cgaagacgtg atgcgcttcc tcgacaacgt gctgcaggac ttcatcgatc gcgcaccgcc
     5281 ggggatggag aaggcgaagt attccgcagc gcgcgaacgg tccgttggcc tcggtgtgat
     5341 gggcttccac tcgttcctgc aagcgcagaa cgtcccgtgg gagagcgtgg tcgccaaggt
     5401 atggaaccgc cggatgttca agcacatccg cgaacaggcc gatatcgcgt cgcgcgtgct
     5461 ggccgaggaa cgtggcccgt gccccgacgc cgccgagtac ggcatcatgg agcggttcag
     5521 tcacaagatg tcgatcgcgc ccaccgcgtc gatttcgatc attgcgggga attcttcgcc
     5581 ggggatcgag ccgatcgcgg cgaatgtgtt cctgcagaag acgctgtccg gcagcttcac
     5641 ggtgcgcaac aggcacctgc agcaattgct ggaagcgcgc ggcatggaca ccgacgaagt
     5701 atggtcctcg atcacgctgc gcagcggca
//
LOCUS       DATGEM010000215         8868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_306189, whole genome shotgun sequence.
ACCESSION   DATGEM010000215 DATGEM010000000
VERSION     DATGEM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306189"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VKQ27_06765"
     CDS             <1..473
                     /locus_tag="VKQ27_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88670.1"
                     /translation="TRGDVDVVQVFEPFASSGDFPVWYAQASRGPTSYTTFYARRGLL
                     TARHDELLRMVRAIHRTLGWVAEADGNAIAATIAGYFPDVPAPTLAQACARYKALGIW
                     GRDPHLPRAGYDRLRASLVSGAFVSPGTDHEVAVDNSLADTVIRREADATRNNR"
     gene            451..1332
                     /locus_tag="VKQ27_06770"
     CDS             451..1332
                     /locus_tag="VKQ27_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY88671.1"
                     /translation="MPHATTDDGVRLYYEETGSGRPVIFVHEYAGDYRSWEPQMRHFG
                     QRCRAITYNARGYPPSDVPEEVAKYSQARAADDIASVLDHLKIDKAHIVGLSMGGFAT
                     LHFGFRHPSRALSLCVAGCGYGAERGQTDKFRAEAETIAAFITDQGMEVFAAKYAYGP
                     TRVQFENKDPRGFDEFKRMLAEHSALGARNTQLGVQRERPSLYDLIDQMKALKLPTLI
                     LTGDEDWPCLAPGLLMKQTIQSAALSVMPNCGHAINIEDPDEFNRIVSAFITQVDAGR
                     WPMRDPRAVSASITGMK"
     gene            complement(1316..1522)
                     /locus_tag="VKQ27_06775"
     CDS             complement(1316..1522)
                     /locus_tag="VKQ27_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88672.1"
                     /translation="MTDGGLGAGECVGIEAKSSGWLVYYSERGSKSPLDTFADEDAAC
                     RALIGHVDRMMRQAGLGGVSISSR"
     gene            complement(1533..2033)
                     /locus_tag="VKQ27_06780"
     CDS             complement(1533..2033)
                     /locus_tag="VKQ27_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TNT domain-containing protein"
                     /protein_id="HLY88673.1"
                     /translation="MPRALSILSLLGLLAACTPPAAQQPASTLPPNIRPDLAEKWYPN
                     GQIMWPPNDGCAAAPVSQTLPAGSMIDRFGSEGGTFFSPRGASYAARAVPYVCSKMAY
                     TVYRVDRPLHVQACRAAPWFDEPGGATQYKTDAPAYKLREAGSIEVATGDSSGNGKPA
                     SPCGSP"
     gene            complement(2115..3224)
                     /locus_tag="VKQ27_06785"
     CDS             complement(2115..3224)
                     /locus_tag="VKQ27_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HLY88674.1"
                     /translation="MHAIRIDRGKPLCDEPNCGHNRYHPDIAPAAEIGEGEEIALETR
                     DALDGQITPATTVADFANLNAGAVHPLTGPVFVKGTQPGDMLEIEFTDIIAQPTAFSA
                     IMPGLGFLRDVMTTPYLVHWEIKDNWATSPQLPGVRIPGAPFMGVSAVAPTAELLAAW
                     TAREQRVIDAGGFALPPDANGAVPAGPCGLAGLRTLPPRENGGNFDVKQLTKGSKLFL
                     PVFKEGALFSTGDGHFAQGDGEVCVTAVEMGATAVVRFKVHKGLASRRKFSAPVFSRT
                     SYFADPRFAAPERFLGVMGMPINAAGEIEAENLTLACRNAVLNMMELLQERGFSREQA
                     YVICSVAVDLRISNVVDVPNYVVSALLPEAIFDGD"
     gene            complement(3339..4394)
                     /locus_tag="VKQ27_06790"
     CDS             complement(3339..4394)
                     /locus_tag="VKQ27_06790"
                     /inference="COORDINATES: protein motif:HMM:NF033816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HLY88675.1"
                     /translation="MANANPFRIAVVWRGDADARRNATPQNNRFFRVFEELAAIGVDA
                     QPAVFDESLAEEVRAQLLAADGVLVWVDPLHDGKDRSVLDALLRDVAANGPWVSAHPD
                     TILKMGTKEVLHRTRHLGWGTDTQLYRTATEFDVEFPARLRSAGPRVLKQNRGNGGQG
                     VWKVEALAGSDAGALVRVLEARRGSLPEDLSLAAFMVRCRGYFAGGGCIIDQPFQPRL
                     PDGMIRCYVGVDRVVGFGHQLIKALIPPPPEGPDSPGAQPGPRIMHGASAEPFQALRA
                     LMDAEWVPQMMQTLGLDRASMPIIWDADFLYGPRTASGEDTYVLCEINVSSVFAIPEQ
                     APAAIARLVRERMMAQR"
     gene            complement(4413..5210)
                     /locus_tag="VKQ27_06795"
     CDS             complement(4413..5210)
                     /locus_tag="VKQ27_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88676.1"
                     /translation="MDVVDLDKKQVVHRITGLREPQGVLYVPLADRLAVASAGDGTLR
                     LFSGDNFAPLRSIALGDDADNVRLAADGRDVLVGYGDGGLAVIDPVRGSLSEKLPLAA
                     HPEGFQLTSDGHVYVNVPDAHDVEVADLKAGKVIATWTLQGMSGNFPMALGEAGIVAV
                     VFRQPPRFVRIDSATGKERSRSAVCGDADDVFFDVRRQRYYVSCGAGGVSAFQVNLTG
                     VEALPSVTTSGGARTSLFVPDLDRLFVAARASLLGSNATIWVMRPEP"
     gene            5275..5421
                     /locus_tag="VKQ27_06800"
     CDS             5275..5421
                     /locus_tag="VKQ27_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88677.1"
                     /translation="MRPDVWGNGIMVSNASGGALSARADAIGMAISIASHPRFLRLIL
                     LRTR"
     gene            complement(5433..6635)
                     /gene="chrA"
                     /locus_tag="VKQ27_06805"
     CDS             complement(5433..6635)
                     /gene="chrA"
                     /locus_tag="VKQ27_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HLY88678.1"
                     /translation="MSVSSPAVFPPRGSVLEVVTYFLRLGATGFGGPVALCGQMEKDL
                     VQGKAWLTKDQMREAIAISQSMPGPLAIQVGIFIAYMRGGFWGAWAGGWSFILPNFIM
                     VAALGAAYVHFGGLSWMTAIFYGVSPAVIALIVHSCWRLAKLGMEDRFQWAVAAAAFV
                     ITAVVRAEMAAVFIGAGMCGLLYYGTAFRRTPTATLPAVAAPLGASGAAIGTAKAASL
                     PLLAKLGGFFLKAGSLTFGSGLVIVPFLEQGLVRETNWLNPREFLVAVAIGMMSPGPV
                     VITATFVGYLVAGFWGSLVATVGIFLPSFLMVLVVAPILMRHRGNANVQGFVKGAFAA
                     AIGTILGASILLGKIAIGDWLTALIALVCLGLLFRFKVNNPLLVGGAAVVGLIAFPIL
                     QPGWVFVQ"
     gene            complement(6632..7456)
                     /locus_tag="VKQ27_06810"
     CDS             complement(6632..7456)
                     /locus_tag="VKQ27_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HLY88679.1"
                     /translation="MPTPDTITAAQLARLIGTPDGPLLIDVRIDTDHAADPRMLPGSV
                     RRDFRTVSTWAGDYTGRAAVVICQRGLKLSQGVAAWLRHAGARAENLEGGFEAWVKAN
                     GLLVRPDHVPPRDSQGRTVWVTRVRPKIDRVACPWLIRRFVDPSAVFLFVAPSEVMDA
                     ADRFKATPFDIEDVFWSHRRDKCTFDVMVDEFGLRSEALDRLACIVRAADTDRLDLAP
                     QAAGLLAASLGLSRMYRDDLAQLEATMPLYDAFYRWSRDAVDELHNWPAPGAGGRP"
     gene            complement(7598..8650)
                     /locus_tag="VKQ27_06815"
     CDS             complement(7598..8650)
                     /locus_tag="VKQ27_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY88680.1"
                     /translation="MQPGILLALASAVLFGASTPFAKLLLGSVDPWLMAGLLYLGAGL
                     GLAVLHVSRGVLRLPAIEAPLRRSDVPWLGAVILMGGVLGPLLLMLGLAHTEAAAASL
                     LLNLEGLATMGLAWVVFHEYVDRRLLLGAFSILAGAALLSWQGGASFQWSGLLIAGAC
                     LCWGIDNNLTRKLSSSDPVQIAMLKGLVAGAVNLALAVSQGAVLPSTGIVLAVGIVGL
                     LGYGVSLALFVLGLRYLGAARTGAYFSLAPFVGAILAIPMLGEPLSAQLIVAGCLMGF
                     GLWLHLAEQHEHEHAHEVLEHEHRHSHDVHHQHEHALDDPTGEPHSHWHRHAPLVHRH
                     PHYPDLHHRHGHKHAN"
BASE COUNT         1762 a         3024 c         2671 g         1411 t
ORIGIN      
        1 tgacgcgcgg cgatgtcgat gtggtgcagg tgttcgaacc cttcgcctcg agcggcgatt
       61 tcccggtgtg gtacgcgcag gcgagtcgcg gtccaacctc ctacacaaca ttctacgcgc
      121 gacgtggcct gctgaccgcg cggcatgatg agctgctgcg catggtgcgg gcgatccatc
      181 gaacgctggg ttgggtggca gaagcggatg gcaacgcaat cgccgcgacg atcgcagggt
      241 acttcccgga tgttcccgcg ccgacgctgg cgcaggcatg cgcgcgctac aaggcactcg
      301 ggatatgggg gcgcgatcca catttgccgc gcgccggtta tgatcggctg cgcgccagcc
      361 tggtgtcggg cgccttcgtc tcgccaggca cggatcatga agtcgcggtg gacaacagtc
      421 tcgccgacac agtcatcagg agggaagcag atgccacacg caacaacaga tgacggagtg
      481 cgtctctatt atgaagagac cggctccggc cggccggtca tcttcgtgca cgaatacgcc
      541 ggcgattacc ggagctggga accgcagatg cggcatttcg gccagcgctg tcgcgccatc
      601 acttataacg cgcgcggcta tccgccctcg gatgtgccgg aggaagtggc gaagtattcg
      661 caggcgaggg cggcggacga catcgcctcg gtactggacc acctgaagat cgacaaagcg
      721 cacatcgtcg gcctgtcgat gggcggcttc gccacgctgc atttcggctt ccgccatccg
      781 tcgcgcgcgc tgtcgctctg tgtagccggc tgtggttacg gcgcagagcg cggccagacc
      841 gacaaattcc gcgccgaagc cgagacgatc gccgcattca tcacggacca ggggatggag
      901 gtgttcgcgg ccaagtatgc ctacggcccg acccgcgtgc agttcgagaa caaggacccg
      961 cgcggcttcg acgagttcaa gcgcatgctg gccgagcatt ccgcgctcgg cgcgcgcaac
     1021 acacagctag gcgtgcagcg cgagcggcca tcgctctatg acctgatcga tcagatgaag
     1081 gcgctgaagc tgcccactct gatcctgacc ggcgatgagg actggccgtg cctcgctccc
     1141 ggcctgctga tgaagcagac catccagagc gcggcgctgt cggtgatgcc gaactgcggc
     1201 catgccatca acatcgagga cccggacgag ttcaaccgca tcgtcagcgc cttcatcacc
     1261 caggtggatg cgggacgctg gccgatgcgc gatccgcgcg cggtatcggc ctcgatcacc
     1321 gggatgaaat agacacgcca ccaagccctg cctgccgcat catccgatcg acgtgaccga
     1381 tcagagcgcg gcaggcggcg tcttcatccg cgaacgtatc aagcggcgac ttgctgcccc
     1441 gctcactgta gtacaccaac cagccggatg atttcgcttc gatgccgaca cactcgcccg
     1501 cgccgagccc gccatcggtc acatagtacc agtcagggac tcccgcacgg cgaagcaggc
     1561 ttgccatttc ctgaactgtc acccgtcgcc acctcgatcg aaccggcctc gcgcagctta
     1621 tacgcggggg cgtcggtctt gtattgcgtc gcgccgccgg gttcatcgaa ccatggcgct
     1681 gctctgcagg cctgcacgtg cagtggtctg tccacgcgat aaactgtgta ggccatcttc
     1741 gaacagacat agggcaccgc ccgggcggcg tagctcgcac cgcgcgggct gaagaaggta
     1801 ccgccctcgc tgccaaaacg atcgatcata gaaccggcag gaagtgtctg cgataccggc
     1861 gccgcggcgc aaccgtcatt cggaggccac atgatctgtc cgttcggata ccacttctcg
     1921 gcgaggtccg ggcggatgtt gggtggcagc gttgatgcag gctgctgcgc ggccggtggc
     1981 gtgcaggcag ccagcagacc gagcaacgat agtatagaga gggcacgcgg caccgtattc
     2041 ctcctgttgt ggttgacgcc gcctgcatag tcgatgtcat ggcaaagtga cggtgtagtc
     2101 gcgtgagacg ggcgtcaatc tccgtcaaat atggcctcgg ggagcagcgc cgacaccacg
     2161 tagttcggca cgtcgaccac gttcgagatc cgcagatcga cagccaccga gcagatcaca
     2221 taagcctgct cgcgcgaaaa gccgcgttcc tgcaacagct ccatcatatt cagcacggca
     2281 ttgcggcaag caagcgtcag gttctcagcc tcgatttcgc ccgccgcgtt tatcggcatc
     2341 cccatcacgc cgaggaaccg ttccggcgct gcaaaccgag gatcggcgaa atagctcgtg
     2401 cgtgagaaca ccggcgcgct gaacttgcgc ctgctcgcca gccccttgtg taccttgaag
     2461 cgcaccactg cagttgcgcc catctcaacg gcggtgacac acacctcgcc atcgccctgc
     2521 gcgaagtgac cgtcgccggt ggaaaacagc gcaccttcct tgaacacagg aaggaacagc
     2581 ttcgaacctt tggtaagctg tttcacgtcg aaattgccgc cgttctcgcg aggcggcagc
     2641 gtgcgcaagc cggccaaccc gcaaggcccc gccggaaccg cgccgttcgc atcgggtggc
     2701 agcgcgaagc cgccggcatc gatcacccgc tgttcgcgcg cagtccacgc cgcaagcagt
     2761 tccgcggtag gcgccacggc ggacacgccc atgaacggcg ccccaggaat ccgcacgccg
     2821 ggaagctgag gcgacgtcgc ccagttgtcc ttgatctccc agtgcaccag gtacggcgtc
     2881 gtcatcacgt cgcgcaggaa cccaagcccc ggcatgatcg cgctgaatgc cgtgggctgt
     2941 gcgataatgt cggtgaactc gatctccagc atatcgcccg gctgcgtgcc tttgacgaac
     3001 accggccccg tcagcggatg caccgcgccg gcattcaggt tggcaaaatc ggcaacggtg
     3061 gtcgcaggcg tgatctggcc atcaagtgca tcgcgcgttt cgagcgcgat ttcctcgcct
     3121 tcgccgatct cggcggcagg cgcgatatcg gggtggtagc ggttgtggcc gcaattcggt
     3181 tcatcgcaga gcggcttgcc gcggtcgata cggatggcgt gcatggcatt atccccgttg
     3241 gcaagccagc tttgcatggt cttgggcgat gcggctacag cggccattct gtgcgacact
     3301 gatgcctggg ggaacaatct taggccttca taccagcctc agcgctgcgc catcatccgc
     3361 tcccgaacaa gccgcgcaat cgccgccggc gcctgctccg gaatagcaaa caccgaactg
     3421 acattgatct cgcacagcac atatgtgtcc tcgcccgatg cggtgcgcgg cccgtagagg
     3481 aaatccgcat cccagatgat cggcatcgac gcgcgatcga ggccgagcgt ctgcatcatc
     3541 tgtggcaccc actccgcatc catcagcgcc cgcagcgcct gaaacggctc tgccgacgca
     3601 ccgtgcatga tacgtggccc cggctgcgcc cccggagaat ctggcccctc cgggggcggt
     3661 ggtatcagcg ccttgatgag ttgatgtccg aagccaacga cccggtccac gccgacatag
     3721 cagcggatca tgccatccgg caggcgcggc tggaacggct ggtcgatgat gcacccgcca
     3781 cccgcaaagt acccccggca acgcaccatg aacgcggcaa gtgacaggtc ctccggcagg
     3841 ctgccgcgac gagcctccag cacccgcaca agcgcaccag catcagaacc ggcaagggcc
     3901 tcgaccttcc aaaccccttg cccgccattg ccacgatttt gcttcagtac tcgaggccct
     3961 gccgatcgca gccgcgccgg gaactcaacg tcgaattccg tcgccgtgcg atagagttgc
     4021 gtatcagtgc cccagccaag atgcctggtg cggtgaagca cctccttcgt gcccatcttc
     4081 aggatcgtgt caggatgcgc gctgacccag gggccattcg ccgcgacatc gcgcaacagt
     4141 gcatcgagga cgctccggtc cttcccatcg tgtagcggat cgacccacac cagcacgcca
     4201 tccgcagcca gaagctgcgc gcgcacctcc tcggcaaggc tttcgtcgaa caccgccggc
     4261 tgggcgtcga cgccgatcgc cgccagttcc tcgaacacgc ggaaaaagcg attgttctgc
     4321 ggggttgcgt tgcgtcttgc atcagcgtcg cctcgccaga caacagcgat cctgaacgga
     4381 tttgcatttg ccatcgatct ctctcccttc agttagggct ccgggcgcat gacccaaatc
     4441 gtcgcgttcg agcccaacag actggcgcga gcggcgacaa acagccggtc aaggtccggc
     4501 acaaacaggc tggtgcgcgc accgcccgat gtggtcacgg acggcagcgc ctctacgccc
     4561 gtgaggttga cctggaatgc gctcacgccc cccgcgccgc agctcacgta ataacgttgc
     4621 cgcctgacat cgaagaaaac atcgtcggca tcaccgcaca cggcgctccg gctgcgttcc
     4681 ttccccgtcg cggaatcgat gcgcacaaat cgcggcggtt gacggaacac cacggcgaca
     4741 atgcctgctt ccccgagcgc cattggaaaa tttcccgaca tgccctgaag cgtccacgtc
     4801 gcgatcacct tgccggcctt gaggtcggca acttccacgt cgtgtgcatc gggtacgttg
     4861 acgtagacgt gtccatccga ggtcaactgg aaaccttccg gatgcgccgc gagcggcagt
     4921 ttctcgctca aggaaccccg caccggatcg atgaccgcca ggccgccatc accgtagcca
     4981 accaggacat cgcgcccatc tgcagcaagc cggacattgt ccgcgtcgtc gccgagagcg
     5041 atgctgcgca gcggcgcgaa gttgtcgcca ctgaacaggc gcagcgtgcc atcgcctgcg
     5101 ctggcgacgg caagcctgtc ggccaacggc acataaagca ccccttgtgg ctcgcgcagg
     5161 cccgtaatcc ggtggacaac ctgtttcttg tccagatcga cgacgtccac ggtaccgttg
     5221 ccaagttccg caacgaacag acgtttccgc gcgagatcga ccgcgaggtg atcgatgcgc
     5281 cccgacgtgt ggggcaacgg gatcatggtc tccaacgcaa gcggtggagc gctatccgcc
     5341 cgtgcagacg cgatcggcat ggcgatcagc atcgccagcc acccgcgatt cctccggctc
     5401 atcctgttgc gcacccgatg acccgccgcg cgctactgaa caaacaccca gccgggctgc
     5461 aggatcggaa acgcaatcag cccaacgacg gcagcgccgc cgaccagcag cgggttgttc
     5521 actttgaaac gaaacagcag gccgaggcac accagcgcga tcagtgcggt gagccagtcg
     5581 ccgattgcga tctttccaag caggatgctc gcgccaagga ttgtaccgat cgcagcggca
     5641 aacgctcctt tgacgaaccc ctgcacattg gcattaccac gatgccgcat caggattggc
     5701 gcgaccacca gcaccatcag gaacgacggc aggaagatgc cgacagtcgc aacaagcgaa
     5761 ccccagaaac cggcaaccag atagccgacg aaagtcgcgg tgatcaccac cggccctggg
     5821 ctcatcatcc cgatcgccac cgccaccagg aactcgcgtg gattgagcca gttggtctcc
     5881 cgcaccagcc cctgctcgag aaacggcacg atcaccaggc cgctcccgaa ggtcaggctg
     5941 ccagctttca gaaagaagcc gcccagtttg gcgaggagcg gcagcgacgc cgctttcgcc
     6001 gtgccgatgg cagcgccaga cgcgccgagt ggcgcagcga ccgccggcag ggttgccgta
     6061 ggcgtgcggc ggaaggctgt gccgtaatac agcaagccgc acatgccagc gccgatgaac
     6121 accgccgcca tctccgctcg cacaacggcg gtgatgacaa aggccgcggc cgcgactgcc
     6181 cactgaaagc gatcctccat gccgagcttt gcgagccgcc agcaggaatg gacgatgagc
     6241 gcaatcaccg ccggactgac gccataaaag atcgccgtca tccaggacag accgccgaaa
     6301 tgcacatagg ccgcacccag tgccgccacc atgatgaaat tcggcagaat gaacgaccag
     6361 cctccagccc atgcgcccca aaatccgccg cgcatgtagg cgatgaagat gccgacttga
     6421 attgcgagcg gtcccggcat ggactggctg atcgcaattg cctcgcgcat ctgatccttg
     6481 gtcagccacg ctttgccttg caccagatcc ttttccatct ggccacacag cgcgacaggg
     6541 ccaccaaaac cggtcgcgcc aagacgcagg aagtacgtga ccacctcaag gacagagccg
     6601 cggggcggga acaccgccgg actggagacg ctcatggccg ccctcccgcg ccaggcgccg
     6661 gccagttatg caattcatcc accgcatcgc gtgaccagcg gtagaatgca tcgtatagcg
     6721 gcatcgtcgc ctcaagctgt gccaggtcat cgcgatacat ccgcgacaat ccaagcgaag
     6781 cagccagcag ccccgcggcc tgcggcgcca ggtccagcct gtcggtatca gcagcccgca
     6841 cgatgcacgc caggcgatcc agcgcttccg agcgcagacc gaactcgtcc accatcacgt
     6901 cgaaggtgca cttgtcgcgg cgatggctcc agaacacgtc ctcaatatca aagggcgtcg
     6961 ccttgaagcg gtctgccgca tccatgacct cggacggcgc cacgaacagg aacaccgccg
     7021 aaggatcgac aaatcgccgg atcagccacg ggcacgccac ccgatcgatc ttcggccgca
     7081 cgcgcgtgac ccagaccgtg cgcccctggc tgtcgcgcgg cgggacatgg tccgggcgca
     7141 ccagcaggcc attcgccttg acccaggcct cgaagccgcc ctccaggttc tccgcacggg
     7201 cgccggcgtg ccgcagccac gccgcaacac cctgactcag cttcaatcca cgttggcaga
     7261 tcaccaccgc ggcacggccc gtataatcgc cggcccaggt tgaaaccgtg cggaaatcgc
     7321 gccggacact gcccggcagc atgcgcggat cagcggcatg gtccgtgtcg atacggacgt
     7381 cgataagcag cggcccgtcg ggcgttccga tcagccgggc caattgggca gcagtgattg
     7441 tgtcgggtgt gggcatggac gtccctctcc tagcgcggag ttcggacgcg atgctctggc
     7501 cgaagcctca cggggtgatc gcggtcaacc ccttgcggcc aatctggtgg gccgtatgtg
     7561 tcttgtcaat ctgcccctgc cctgagagtg cgtgccctca atttgcgtgc ttgtgaccgt
     7621 ggcgatgatg caggtcaggg taatgcgggt gacggtgcac cagcggcgca tggcgatgcc
     7681 aatggctatg cggctcgccc gtcggatcat ccagggcatg ctcgtgctga tgatgtacgt
     7741 catggctatg ccgatgctcg tgctcgagca cctcgtgggc atgctcatgc tcgtgctgct
     7801 cggccagatg cagccatagg ccgaagccca tcaggcagcc cgccacgatc agctgtgcgg
     7861 atagcggttc gcccagcatc gggattgcca ggatcgcgcc cacgaacggc gcgagcgaga
     7921 aatatgcccc cgtgcgtgcc gccccgagat agcgcagccc gagcacgaac agcgcaaggc
     7981 tcactccgta gcccaacagg ccgacgatac cgacggcaag tacaatgccg gtcgaaggca
     8041 gcaccgcacc ttgcgagacc gcgagcgcaa gattgaccgc gccagcaacc aatcctttga
     8101 gcatggcaat ctgcacagga tcggacgacg acagctttcg cgtcaggttg ttgtcgatac
     8161 cccagcacag gcaggcgcct gcgatcagca gcccgctcca ctggaaggaa gcgcccccct
     8221 gccaggacag cagggcagcg ccggccagga tggagaaagc acccagcagc aggcggcgat
     8281 cgacatattc gtgaaagacg acccacgcca gccccatcgt cgcaaggcct tcgagattca
     8341 gcagcagcga agcggccgct gcctccgtgt gcgcgaggcc aagcatcaga agcagcggcc
     8401 caagcacgcc ccccatcagg atcaccgctc cgagccaagg cacatccgac cgccgcagcg
     8461 gcgcttcgat cgccggcaac cggagaacgc cgcgcgacac atgcagcacg gcaaggccga
     8521 ggcccgcgcc gagatagagc aaccccgcca taagccatgg atcgaccgag ccaagcagca
     8581 gcttggcgaa cggcgtgctt gcgccgaaca gaacggccga cgcgagagcg agcaggatac
     8641 ccggttgcat gtccggcagc ctacaccgtg ttgccccgaa agcggaccag ctctacggcg
     8701 gcgccacgaa ctgccgccag tgctgatccg gcagcccctc gacatgactc gtcgtccggt
     8761 caccataccg ttctgtcgtg ccttggcgtc accgcgccgg acaatcgcca ctctgcaaca
     8821 ccatcgccgc gcctcgcgat gtccaaccct gctgaacgat ggaaccac
//
LOCUS       DATGEM010000216         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_259403, whole genome shotgun sequence.
ACCESSION   DATGEM010000216 DATGEM010000000
VERSION     DATGEM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259403"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>3647
                     /gene="gltB"
                     /locus_tag="VKQ27_06820"
     CDS             13..>3647
                     /gene="gltB"
                     /locus_tag="VKQ27_06820"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HLY88681.1"
                     /translation="MDARADTLPLDLHPVLTVGGSDSTSLDEVVELLAQNGRTLAEAM
                     RMSVPPANHGNKSSFLQYTGDCVEPWDGPAALAFSDGRQVGAILDRNGLRPCRFAVDQ
                     DGLVVAGSEAGLVDMDPEKIVHSGRLGPGQMILADLETHEFLENDELLTRFDQNGRYK
                     DMVQLDIPLLPSEDVSAPLDASELNRLQHRFGYTREEVRMILAPMAAEGKDAVWSMGD
                     DTPLAPLARAPRPVYAFFRQRFAQVTNPPIDPLRETVVLQMHTRLGPWPHLFEEPSAP
                     LPGLSLSSPLLSLGQMHALKHKQHQQAEALPLETVDCLFSPNESLEDALDRLTRRAVE
                     LVSDHAMILLLTDRNASPTALPIPMAMALGAIHRSLTVAGVRTEVGLAVEAGDCRDIH
                     HVAVLLGMGAGAVCPWLALETARSLSPESGEENTLHALQLGLAKVMSKMGISVVDSYR
                     GAHLFDAIGLADEVIDKCFFGTPSPLSGIGFPEIEKQIRQFWIDKPPASEPCDDAAQE
                     DGGTLVAAIAPVKDLPDYGFVRFRKADEAESHAWQPQTVRALQAAMGSTKQGAALALA
                     PWSAFTSQASELQPAHLRDLLEIRPAGPELELETVEAPGSIVKNFIASAMSFGSLSPE
                     AHQTITQAMNLLGARSNTGEGGEDPAVYAPIDGAPSLLNNKIKQVASARFGVTAEYLI
                     HAEEIEIKIAQGAKPGEGGQLPGHKVTELIARLRHAQPGMQLISPPPHHDIYSIEDLA
                     QLIYDLKRVNPRAAVGVKLVSECGVGTVAAGVAKAYADYIVIAGHTGGTGASSLSSIK
                     YAGDPWELGLAEAQQVLIHNRLRGRVRLRTDGGLRTARDILIASLLGADEFAFGTSVL
                     VALGCDMARQCHLNTCPTGIATQKPELRAKFRGKPEHVVRFFDELAHELQQLLASLGL
                     PSLAAATGRTDLLEQVRFEGNLHLEPVLAASKLAEKNDSPIRWQGQRNDRPEDHPALD
                     DAWVEPAFAAYKSNQPYSVTTHITNEHRTVGARLAGEIAQFRTNNPTASGSALQFDLK
                     GVAGQSFGAFAVAGMTLKLEGLANDFVGKGLCGGELILRGEGRAARESTKHTILGNVA
                     LYGATSGSLYAAGRAGERFAVRNSGALAIVEGVGDHGCEYMTGGLVVALGDTGINFGA
                     GMTGGLAWVYDDKAAFIEKGMYHKDFLIAERFDDLDYTAQRSIYELVELHAAKTSSSC
                     ANVLLTQ"
BASE COUNT          655 a         1287 c         1042 g          663 t
ORIGIN      
        1 aaccgcgcgc gcatggacgc ccgcgccgat acgctccctc tcgatctgca tccggtcctg
       61 accgtcggcg gctccgattc cacatcgctc gatgaagtag tagaactact cgcgcagaac
      121 ggccgcaccc ttgccgaagc catgcgcatg tctgtgccgc ctgccaacca cggcaacaag
      181 tcctcgttcc tgcaatacac tggcgattgc gtcgagccct gggacggtcc cgcagccctg
      241 gccttttccg acggccgcca ggtcggagcc attctcgatc gcaacggctt gcgcccttgt
      301 cgcttcgccg tcgatcagga tgggctcgtc gtcgccggtt ccgaggcagg tctggtcgac
      361 atggatccgg agaagatcgt ccactccggc cgcctgggcc cagggcagat gatcctggcc
      421 gatcttgaga cccacgagtt cctcgaaaac gacgaactgc tcacgcgctt cgatcagaat
      481 ggccgctata aggacatggt gcagctggat attccactgc tgccttcgga ggacgtctct
      541 gcgcctctcg acgcgtcgga gctgaatcgt ctccagcacc gcttcggcta tacccgcgaa
      601 gaggttcgga tgatcctcgc gcccatggcc gccgaaggca aagacgccgt ctggtccatg
      661 ggcgacgata cgccgcttgc gccactggct cgggctccgc gccctgtcta tgcgttcttc
      721 cgccagcgtt tcgcccaggt caccaatccg cccatcgatc cgttgcgcga aaccgtcgtg
      781 ctgcagatgc atacccgtct cggtccctgg ccccatcttt ttgaagagcc aagcgcgccg
      841 ctgcccggcc tgtcgctgag ttcgccattg ctctcgctgg gccagatgca tgcccttaag
      901 cacaagcagc atcagcaggc tgaggcgctt ccgctcgaaa ccgtcgattg cctcttctct
      961 ccgaacgaat cgctcgaaga cgctctggat cggctgaccc gccgcgccgt cgaactcgtg
     1021 tccgaccacg ccatgatcct gcttctgacc gatcgcaacg cctctcccac ggctcttccc
     1081 atccctatgg ccatggcgct gggcgcgatc catcgctcgc tgaccgttgc cggcgtgcgc
     1141 actgaggtag gtcttgccgt cgaagccggc gattgccgcg acatccatca cgtcgcggta
     1201 ctgctcggca tgggcgccgg agcagtctgt ccctggttgg cgctcgagac cgcccgcagc
     1261 ctctctccgg agagcggcga agaaaacacc ctgcacgcgc tccaactcgg cctcgcgaag
     1321 gtcatgtcca agatgggcat cagcgtcgtc gacagctacc gcggcgcgca tctcttcgac
     1381 gccatcggcc tggccgacga agtgatcgac aagtgcttct tcggaacgcc gtctccgctc
     1441 tcgggcatcg gctttcctga gatcgaaaaa cagatccgcc agttctggat cgacaagccg
     1501 cccgcttccg aaccttgcga tgatgctgcg caagaagatg gcggcacgct cgttgctgct
     1561 atcgctccgg tcaaggatct tcccgactac ggtttcgtgc gcttccgcaa agccgatgag
     1621 gcggagtcgc atgcctggca gccgcaaacc gtgcgcgcac tgcaggctgc gatgggctcg
     1681 acaaaacaag gcgcggctct tgctctcgcg ccatggtcgg ccttcacttc gcaggccagc
     1741 gagctgcagc ccgcgcacct ccgcgacctg ctcgaaatcc gccccgccgg cccagaactt
     1801 gagctcgaga cagtagaagc tccgggcagc atcgttaaga atttcatcgc ctcggccatg
     1861 agcttcggct cgctgtcgcc tgaagcgcac cagaccatca cccaggccat gaacctgctc
     1921 ggcgcacgct ccaacaccgg tgagggcggc gaagacccgg cggtctatgc accgattgac
     1981 ggcgcgccct cgctgctgaa caacaagatc aagcaggtgg ccagcgcgcg attcggtgtc
     2041 acggcggaat acctgattca cgccgaagag atcgagatca agatcgctca gggcgccaag
     2101 cccggcgaag gcggccagct ccccggtcac aaggtcaccg agctgattgc acgcctgcgc
     2161 catgcccagc ccggcatgca gctcatcagt ccaccgccgc atcacgacat ctattcgatc
     2221 gaagacctcg cccagctcat ctatgacctc aagcgcgtca atcctcgcgc cgcggtcggc
     2281 gtcaagcttg tctccgaatg cggcgtgggc acggtcgctg caggcgtagc caaggcgtat
     2341 gcggattaca tcgtcatcgc cgggcatact ggcggcacgg gcgcatcgtc gctctccagc
     2401 atcaagtacg cgggcgaccc atgggagctc ggtcttgccg aggcacagca ggtgctcatc
     2461 cacaaccgtt tgcgcggccg tgtccgtctg cgtaccgacg gcggcctccg aaccgcacgc
     2521 gacatcctga tcgcttcgct gctcggagcc gacgagttcg ccttcggcac ctcggtcctt
     2581 gttgccctgg gctgcgacat ggcgcgtcaa tgccacctca acacctgtcc caccggcatc
     2641 gccacgcaga agccagagct gcgcgccaag ttccgcggca agcccgagca tgtcgttcgc
     2701 ttcttcgacg agttggccca cgagctgcag cagttgctag cctcgctcgg tctgccttcg
     2761 cttgccgccg caaccggccg caccgatctg ctcgaacagg tgcgcttcga aggaaacctg
     2821 cacctcgaac cggtcctcgc cgcttccaag ctggccgaga agaacgattc gccgattcgt
     2881 tggcaaggcc agcgcaacga tcgccctgaa gaccatcctg ctctcgacga tgcctgggtc
     2941 gagccggcct tcgccgccta caaatccaat cagccctact cggtcaccac gcacatcacc
     3001 aatgaacatc gaacagtggg tgcgcgtctt gccggcgaaa tcgcccagtt ccgcaccaac
     3061 aatcccaccg cctccggctc tgccttgcag ttcgatctga agggcgtcgc cggccagtct
     3121 ttcggagcct tcgccgtcgc cggcatgacc ctgaagctgg aaggcctggc caacgacttt
     3181 gtcggcaagg gcttgtgcgg tggcgaactg atcttgcgag gcgagggccg cgcggcccgt
     3241 gaaagcacca agcatacgat cctcggtaac gtcgccctct acggcgcgac ctccggctct
     3301 ctctatgccg ccggccgcgc cggcgagcgc ttcgccgtcc gcaactcagg cgcgctggcc
     3361 atcgtcgaag gcgtgggcga tcacggctgc gaatacatga ccggcggcct ggtggttgca
     3421 cttggcgaca ccggcatcaa cttcggcgcc ggcatgaccg gcggcctggc ctgggtctac
     3481 gacgacaaag ccgccttcat cgaaaaaggc atgtatcaca aggactttct gatagcagaa
     3541 agattcgacg atctggacta taccgcgcag cgctccatct atgaactggt cgaactgcac
     3601 gccgccaaaa cctccagctc ctgcgccaac gtcctcctga cccagtg
//
LOCUS       DATGEM010000217         6777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_95770, whole genome shotgun sequence.
ACCESSION   DATGEM010000217 DATGEM010000000
VERSION     DATGEM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95770"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /gene="rph"
                     /locus_tag="VKQ27_06825"
     CDS             <1..575
                     /gene="rph"
                     /locus_tag="VKQ27_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HLY88682.1"
                     /translation="FMRNSGLGWVTAEYGMLPRATHTRGEREAARGKQSGRTQEIQRL
                     IGRALRAVVDRSQMGEVSITLDCDVLNADGGTRCAAITGAWVALSLAFRHMRKMNVIK
                     TIPLIGQVAAVSCGISGGEPVLDLDYEEDSSAEADANFVLTDSGGIVEIQGTAEKTAF
                     TETQFLGMLTLARHGTAALFEAQRQAATAT"
     gene            572..1186
                     /gene="rdgB"
                     /locus_tag="VKQ27_06830"
     CDS             572..1186
                     /gene="rdgB"
                     /locus_tag="VKQ27_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HLY88683.1"
                     /translation="MMARTLDRGTRLVLASHNPGKLAELGKLLRPFGIDVVSAGALGL
                     AEPPEDAPDFVGNAHIKALAAAQASSLPALADDSGFCVAALNGAPGIYSARWAGAAKD
                     FAAAMARVNREIGGAADRRAWFVAALCLAWPDRHTETFVGRVDGAAAWPPRGTHGFGY
                     DPMFVPAGCTTTFGEMTAAEKHTLSHRARAFSHLIAACFKQCGS"
     gene            1174..1833
                     /gene="tsaB"
                     /locus_tag="VKQ27_06835"
     CDS             1174..1833
                     /gene="tsaB"
                     /locus_tag="VKQ27_06835"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLY88684.1"
                     /translation="MRILTLDAALARCSAAIVADQNTVALRQEKASRGHAALLPVMAE
                     AVLEEARLRASDLDLVAVTVGPGSFTGIRAGLALAHGIGRAAGIAVVGVTVGEALADS
                     LPYLGARELWSVVDSRRDRIFLERGGTTIALALADLLQPSGRIAVAGDAAPTVAAWLA
                     ARDIDVMLTDARIPQARHIAVVAEHRHAGRLPPRPAQPLYVDPPEAKLPAGGLRPAPV
                     G"
     gene            1866..2255
                     /locus_tag="VKQ27_06840"
     CDS             1866..2255
                     /locus_tag="VKQ27_06840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY88685.1"
                     /translation="MAAIHAAAFPPNEAWGLDAISLQLALPGAFGLIDERGGMLLGRV
                     TVDEAEVLTLAVAPEARRQGIARALLETAHKETRLRGGRTIFLEVAVGNTPAHELYRR
                     CGFTEVGRRHHYYPDLSDALVLRMNVS"
     gene            complement(2268..2513)
                     /locus_tag="VKQ27_06845"
     CDS             complement(2268..2513)
                     /locus_tag="VKQ27_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HLY88686.1"
                     /translation="MIDKQAADQELDITSEVCPMTFVRTRLALDRMQAGQTLLVHLRG
                     EEPLRNVPRTAREQGHEVLSLDTDAAGVAHLLLRRGG"
     gene            2610..3023
                     /locus_tag="VKQ27_06850"
     CDS             2610..3023
                     /locus_tag="VKQ27_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HLY88687.1"
                     /translation="MPDTTPNNNVLGLTAQIVSAHVSNNTVNPDSLPSLIQEVYKTLA
                     GVGKEPVSPERPQPAVPVKKSILPDYIVCLEDGKKLKMLKRHLKTSYNMTPEQYRERW
                     GLGAEYPMVAPNYARHRSSLAKKIGLGTKPRSGRR"
     gene            3227..3742
                     /locus_tag="VKQ27_06855"
     CDS             3227..3742
                     /locus_tag="VKQ27_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HLY88688.1"
                     /translation="MESRIERLCVERGLKMTGQRRVIARVLSEASDHPDVEELYRRAS
                     ALDSRISIATVYRTVRLLEEKGILERRDFGGGRARYEPTEHGHHFHLIDIETGKVIEF
                     QDPEYERLMQIMAERLGFDLVSLRLELFGRRREPVQPVRDDPARTRGARRTAAPGHET
                     DKAMDHGRAAR"
     gene            3739..4575
                     /locus_tag="VKQ27_06860"
     CDS             3739..4575
                     /locus_tag="VKQ27_06860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HLY88689.1"
                     /translation="MTVEQMLAKTRADSLPFPELRAGNLGVRIASTAAEIDAVQALRY
                     RVFYEEMGAEPDAVARGTHRDRDGFDAVADHLLVVDHAIGSGAEGVVGTYRLIQREAA
                     ARLGHFYSADEYDITPIEAFPGRILELGRSCVDAAYRSRVAMQLLWRGIAAYVFHYQI
                     DLMFGCASLHGTDPDALAPELTYLYYNHLAPPALRPRALPHRYVDMRRLLPNRVDAKR
                     VQAQLPPLIKGYLRLGGFVGDGAVIDRQFNTIDVAVVVKTDLVTDKYYRHYERQLRDA
                     LE"
     gene            4751..6547
                     /locus_tag="VKQ27_06865"
     CDS             4751..6547
                     /locus_tag="VKQ27_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HLY88690.1"
                     /translation="MEQGEIGPGKDRVAQSAARLLRKTVFAGLLGATAVGPMAAAQAP
                     PPSPATLVPQGSPIPRVLPPAPPSVSPGTAIPLPVSPSGEVPNKPVRVDTVDVEGVTA
                     FPKPDIMGLATGLTGPAVPLPKIDAARQAILQRYRGDGFVLTTVSVRLDSNYHLHFLV
                     TEGRIASVKLDGDIGPAGTQVLRFLNKLTEKQPIDSVTLERYLLLAQDVPGISLRAVL
                     EPSTDQPGALNLIAQVSRQAVSGLASIDNRAFNETGPIEILGLLDFNSFTSYGEKAEL
                     SYYHTLPNSENFGQASLESFIGASGLKGKIYAGYGVTVPTGSLGAIGYNGTTTVFGGQ
                     ISYPIIRSRQQTLNVYTALDGIESNITTTASGPRLQASYDSLRVLRFGEDYALSDVWL
                     GGNRSAINQVSGRISQGLRILGAATNPTARLGERTDFFKANFQTTRTQTLFTPWDGAS
                     VALMGLLAGQWTNDILPPSEQFYLGGSQFTRGYYAGQVPGDKALAATVELQLNTGFEM
                     SMFDKSFDISQQFYLFYDWGETWQNLSTDHAAMINSAGGGVRTQLTRYVEVDFEGLAR
                     FNRFPNGGSSGGASGVSPLYGGAFYWRVLTRF"
     gene            6573..>6777
                     /locus_tag="VKQ27_06870"
     CDS             6573..>6777
                     /locus_tag="VKQ27_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88691.1"
                     /translation="MANGRRKDKVRLLGVVRRARPALMMSTALQATVSLVLALPAHAQ
                     PAPNARPTGGVVVGGSASISQTAN"
BASE COUNT         1207 a         2284 c         2108 g         1178 t
ORIGIN      
        1 cgttcatgcg caactccggc ctcggctggg tcaccgcgga atacggcatg ctgccgcgcg
       61 ccacacatac gcgaggcgag cgcgaggcgg cacgcggcaa gcagtccggc cgcacccagg
      121 agatccagcg cctgatcggc cgcgccctcc gtgcggtggt ggaccgcagc cagatgggtg
      181 aagtctccat caccctcgac tgcgacgtgc tcaatgctga cggcggcacc cgctgcgccg
      241 caatcaccgg cgcctgggtg gcgctcagcc tcgccttccg ccacatgcgg aagatgaatg
      301 tcatcaagac catcccgctc atcggccagg tcgccgcggt gtcctgcggc atctccggtg
      361 gcgaaccagt gctcgatctg gactacgaag aagattcctc ggccgaagcc gacgccaact
      421 tcgtgctcac cgacagcggc ggcatcgttg aaatccaagg cacggcggag aagaccgcat
      481 tcaccgaaac acagttcctc ggcatgctga cccttgcgcg ccacggcacc gctgcactgt
      541 tcgaggccca gcgccaggca gccacggcaa cctgatggcg aggacgctcg accgcggcac
      601 gaggctggta ctggccagcc ataatcccgg gaagctggcg gaactcggca agctgcttcg
      661 ccccttcggt atcgatgtcg tctccgctgg cgcgctcggt ctggcggaac cacccgagga
      721 cgctcccgat ttcgtcggca atgcccacat caaggccttg gctgccgcgc aagcgagcag
      781 cctgccggca ctcgccgacg attccggctt ttgcgtcgcc gctctaaatg gggcgccggg
      841 gatctactcc gcccgttggg caggcgccgc caaggacttc gccgccgcca tggcgcgggt
      901 gaaccgcgag attggcgggg ccgccgaccg ccgggcgtgg tttgtcgccg cgctctgcct
      961 cgcctggccg gaccgtcaca ccgaaacctt cgtcggccgt gtcgacggcg ccgctgcctg
     1021 gccgccgcgc ggcacccacg gcttcggcta cgacccgatg tttgttcccg caggctgcac
     1081 gaccacattt ggcgaaatga cagcggccga gaaacacacg ctcagccacc gtgccagggc
     1141 gttttcccat ttgattgccg cctgcttcaa gcaatgcgga tcttaacgct ggatgccgcg
     1201 ctggctcgct gcagcgcggc catcgtagcc gatcagaaca ctgtggccct ccggcaggag
     1261 aaggcatccc gcggccacgc cgcgctgctt ccagtcatgg cggaagccgt gctcgaggag
     1321 gccaggctgc gtgcgtctga cctcgatctc gttgccgtta cggttgggcc cggcagcttc
     1381 accggcattc gggccggcct cgccctggcc cacgggatcg gccgcgccgc tggcatcgct
     1441 gtcgtcggtg tcaccgtcgg cgaggcactc gccgattcgc tgccctacct gggagcgcgc
     1501 gagctctggt cggttgttga cagccgccgt gaccggatct tcctggagcg cggcggcacg
     1561 acaattgcgc tcgccctcgc agacctgctc cagccgtcag gcaggatcgc ggtcgccggc
     1621 gatgcggccc cgaccgtcgc cgcctggctc gccgcgcgcg acatcgacgt catgctgacg
     1681 gacgcacgta tcccgcaggc gcgccatatc gcggtggtcg ctgaacaccg ccatgcaggc
     1741 cggctcccgc cacgtcccgc ccaaccgctc tatgtcgatc ccccggaagc gaagctgccc
     1801 gcgggaggcc tccgcccggc tccagtcgga tgatcaaact cgcctcccag gcgcatgtgc
     1861 ccgcgatggc ggcgatccac gcggcagcct tcccaccgaa tgaagcctgg ggcctcgatg
     1921 ccatatcgct gcaactcgcc ctgcccggcg cattcggcct catcgacgag cgcggcggca
     1981 tgctgctcgg ccgtgttacc gtggacgagg ccgaggtcct gaccctggcc gtcgctccgg
     2041 aagcccggcg ccagggcatc gcccgtgcgc tcctcgaaac tgcccacaag gaaacccggc
     2101 tccggggcgg ccgaaccatc ttcctcgaag tcgcagtggg caatacgccg gcacacgagc
     2161 tctaccgccg atgcggcttc accgaggtcg gtcggcggca tcattactat cccgacctgt
     2221 cggatgcctt ggtgctgcgt atgaatgttt cgtgactggc ggcccgctca gccgcctcgc
     2281 cgaagcagca gatgcgccac gccagccgca tcggtgtcca gcgacagcac ctcatgcccc
     2341 tgctcgcgtg cagtgcgtgg aacattccgc aacggttctt ctccgcgcag atgcaccagc
     2401 aaggtctgcc ctgcctgcat ccgatccagc gccagcctgg tacgcacaaa ggtcatcggg
     2461 cagacttcgg aagttatgtc caattcctga tccgcggctt gcttgtctat cattttattg
     2521 ccctgctcgt agacacgcca aatatccatt gcgaaacggc catatgcgcc cctataaggc
     2581 gccagtctta ctcccgggaa ggaaccgcaa tgcctgacac gacgcccaat aacaatgttc
     2641 tgggcctgac ggcgcaaatt gtctcggcgc acgtgtcaaa caatacggtc aacccggact
     2701 ctctgccgtc gcttatccaa gaagtgtaca aaacgctcgc cggcgtaggc aaggaaccgg
     2761 tctctcccga gcgccctcaa cccgcagtcc cggtaaagaa atcgatcctg cccgactaca
     2821 tcgtctgcct ggaagacggc aagaaactga agatgctcaa gcgccacctg aagacctcct
     2881 acaacatgac gccagagcaa tatcgggagc gctggggcct cggcgccgaa tacccaatgg
     2941 ttgcccccaa ctatgcgcgt caccgctcct ccctggccaa gaagatcggc ctcggcacca
     3001 agccccgctc cgggcggcgc tagcagataa ttgggattct acgggctgcg cccttagccg
     3061 ggatgcaagg ggcggagcct cttgccgggt gtcggggccg agcctccgat cctttccgtc
     3121 ccggcgaccg cacacgcgag ggttggacag ccgcgacccg gatcggctac gccgtggggc
     3181 tggtccgggg tggccgggac gggctaggtt gaacgcaggg aatccgatgg aatcgcgcat
     3241 cgagcgcctg tgcgtcgaac gaggcctcaa gatgacgggg cagcggcggg tcattgcccg
     3301 cgtcctatcc gaggcctccg atcatccgga tgtcgaggag ttgtaccgcc gggccagtgc
     3361 actcgactcc cgaatctcga tcgccacagt gtatcggact gtgcgactcc tggaagaaaa
     3421 gggcatcctg gagcgacgcg actttggcgg cggccgggcg cggtacgagc ccaccgaaca
     3481 cggccaccat ttccatctga tcgacatcga gaccggcaag gtgatcgagt tccaggaccc
     3541 ggagtacgag cggttgatgc agatcatggc agagcggctt ggtttcgacc tggtctcgct
     3601 gcgcctggag ctgttcggcc ggcggcggga gccggtgcag cctgtgcggg acgatcccgc
     3661 gcgcacgcgc ggcgcgcggc ggactgcagc tcccgggcac gagacggaca aggcgatgga
     3721 tcacggaagg gcagcgcgat gacagtcgaa cagatgctcg cgaagacccg cgccgatagt
     3781 ctgccgtttc ccgaactccg cgcaggcaat ctgggagtga ggattgcatc cacggccgcc
     3841 gagatcgatg cggtccaggc gctgcgctat cgggtgttct atgaagagat gggggcagag
     3901 ccggatgccg ttgcccgcgg cacacaccgt gaccgtgacg ggttcgacgc cgtggccgac
     3961 catctgctgg tggtcgacca tgccatcggc tcgggcgccg agggcgtcgt tggcacctac
     4021 cgcctgatcc agcgcgaggc ggccgccagg ctgggacatt tctactcggc agacgagtac
     4081 gacatcacgc ctatcgaggc gtttcccgga cgcatcctgg agcttggccg ctcgtgcgtt
     4141 gacgccgcct atcgcagccg ggtggccatg caactgcttt ggcgcgggat cgccgcctac
     4201 gtctttcact accagataga tctgatgttc ggctgcgcca gcctgcacgg caccgatccc
     4261 gacgcgctcg cgccggaatt gacctatctc tattacaacc acctcgcgcc tccggctctg
     4321 cggcccaggg ccttgccgca ccgctacgtg gacatgcgcc ggctgctccc gaatcgggtg
     4381 gacgcgaagc gggtccaggc gcagttgcca cccctcatca agggttacct ccgccttggc
     4441 ggcttcgtgg gtgatggcgc ggtgatcgac cgccagttca acaccatcga cgtcgccgtg
     4501 gtcgtgaaga ccgatctggt cacggataaa tattatcgcc actacgagcg ccagctccgc
     4561 gacgcgctgg agtgaccgcc tgccgccccg gtcattgccc atccggcgcg aagaattgat
     4621 cgtaaaaatt gcctatcggc atcagaaact gcataaggat gtcgcggctt cgcattgacg
     4681 aagctgcaac gtctctccga atctgcaccc ggagtactcc tccggttcgc ccgggacaaa
     4741 aggaagaagc gtggagcaag gggaaatcgg tccaggcaag gaccgggtcg cgcagtctgc
     4801 cgcacgcctc ctgcggaaga cggtgtttgc tgggctgtta ggcgcaaccg cggtcgggcc
     4861 gatggcagcg gcacaggcac cgccaccgtc accggccacc ctggtgcctc aaggttcgcc
     4921 aatcccacgc gtgctgccgc cggcaccgcc ctcggtgtcg cccgggacag cgataccact
     4981 gccggtttcg cccagcggcg aggtgcccaa taagccggtc cgcgtcgaca ctgtggacgt
     5041 cgaaggcgtc accgcctttc ccaagccgga catcatgggc cttgccaccg gcctcaccgg
     5101 gccagcggtt ccgctgccaa agatcgacgc ggcccgccag gcgatcctgc aacgctaccg
     5161 cggcgacggg ttcgtgctga ccaccgtctc ggtccgcctc gacagcaatt atcacctgca
     5221 cttcctggtc accgaagggc gcatcgcgag cgtcaagctc gatggcgata tcggtccggc
     5281 cggcacgcag gtgctgcgct tcctcaacaa gctgaccgaa aagcaaccaa tcgattcggt
     5341 gactttggag cgctacctgc tgctggcgca ggatgtccca ggcatcagtc tgcgtgcggt
     5401 gctggagcca tccaccgatc agccgggggc gctcaatctg attgcccagg tgagccggca
     5461 ggccgtctca ggcctcgcca gtatcgacaa ccgtgcattc aatgagaccg gtcctattga
     5521 aatcctgggt ctgctcgact tcaacagctt tacgtcatac ggcgaaaagg ccgagctgtc
     5581 ctactaccac acgttgccga acagtgagaa tttcggtcag gcatcgctgg aatcattcat
     5641 cggagcgagc gggttaaagg gaaagatcta cgcaggctat ggcgtgacgg tcccgacggg
     5701 ctcgctgggt gcgattggct acaacgggac cacgactgtg tttggcggcc agatcagcta
     5761 tccgatcatc cgatcgcgcc agcagacgct gaatgtctat accgcgctcg acggcatcga
     5821 atcgaacatc accaccaccg catccgggcc gcgattacag gcgagctatg actccctccg
     5881 cgtactgcgc ttcggcgagg attatgcgct atccgacgtc tggctgggcg gcaatcgcag
     5941 cgcaatcaat caggtgtcgg ggcgcatatc gcaaggcctg cgcatactcg gcgctgctac
     6001 caatccaaca gctcgcctcg gcgaacgcac cgacttcttc aaggcgaact tccagacaac
     6061 gcggacccag acactcttca caccatggga tggcgcgagc gtcgctttga tgggactgct
     6121 ggccgggcaa tggaccaacg acatactacc gccctcagag cagttctatc ttggcggatc
     6181 acaattcacc cggggctatt acgccgggca ggtgccaggc gataaagcgc tggcggcgac
     6241 ggtcgaattg cagctaaaca ctggtttcga gatgtcgatg ttcgacaagt cgttcgacat
     6301 atcgcaacaa ttctacctgt tttacgattg gggtgagacc tggcagaatc tcagcacgga
     6361 ccatgctgcc atgatcaact cggccggtgg gggcgtgcgc acgcagctta cccgctatgt
     6421 cgaggtcgat tttgaaggct tggcgcgctt caaccgtttc ccgaatggcg gctcgagcgg
     6481 tggcgcgagt ggcgtttcgc cattgtatgg tggtgcgttt tattggcggg tcctgacacg
     6541 attctagccg gtatcgcggg ggactttttt tgatggctaa cgggcgccgc aaggacaagg
     6601 tgaggctgct cggcgtcgtg cggcgggctc gtcccgccct gatgatgagc accgccttgc
     6661 aggcaaccgt gtcgctggtg ctcgccctgc cggcccacgc gcaacccgcg cctaacgcgc
     6721 ggcccaccgg tggcgtggtg gtcggcggct ccgcctcgat cagccagacc gcgaata
//
LOCUS       DATGEM010000218         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1148332, whole genome shotgun sequence.
ACCESSION   DATGEM010000218 DATGEM010000000
VERSION     DATGEM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148332"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VKQ27_06875"
     CDS             <1..842
                     /locus_tag="VKQ27_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88692.1"
                     /translation="AFTLPEGSVDSHCHVFGPGDVFPYAPERKYTPCDAPKETLWALR
                     DQLGVSRNVIVQASCHGADNRALVDALKHSGGRARGVATVKRSVTDEELKALHAAGVR
                     GARFNFVKRLVDALPRDALSEIAARLAPLGWHIVIYFEAQELPELYDFFTSLPGTVVV
                     DHMGRPDVTKSVDGPEFGLFQKLMRENPNFWSKVTCPERLSRLDPDLYDDVVPFARRL
                     VEEFPDRVLWGTDWPHPNLTTYMPDDGKLVDYVPKIAPTADLQRQLLVENPMRLYWPE
                     ECA"
     gene            1111..>2011
                     /locus_tag="VKQ27_06880"
     CDS             1111..>2011
                     /locus_tag="VKQ27_06880"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HLY88693.1"
                     /translation="MEVSFLDYGGAITRVVVPDRQGHMGNVVLGLPDAEAYRKSTSRH
                     GSIVGRYAGRIAGAQLVIDGRTIHLVPGPRGPTLHSDPDSFDHRLWQGRDFADKESLG
                     RVLTLHCPDGDQSYPGRLDVQVTYRLMRASNEFRIEYEARSSEPTAINLTNHAYFNLA
                     GAGAVGVSSHKVTIPADRYAALDELHIPTGDLPSVAGGDLDFRDGRVLGDKPSYDDSL
                     FLGAGGGLKPAALVEEPGSGRRLEISTTEPSIQIYTANSFNGTEMGAEGHCYHRNDGV
                     ALETQHLPDSPHHPDFPSTILRPG"
BASE COUNT          354 a          652 c          639 g          366 t
ORIGIN      
        1 cggccttcac gttgccggag ggttccgtcg actcgcactg ccacgtcttc gggccgggcg
       61 acgtatttcc ctatgcgccc gagcgaaaat acacgccgtg cgatgcgccg aaggagacat
      121 tgtgggcgtt gcgcgaccag ctgggggtct cgcgcaatgt gatcgtccag gcgtcgtgcc
      181 acggtgccga caatagggct ttggttgacg cgctcaaaca ttctggcggg cgggcacgcg
      241 gtgtggcgac cgtcaagcgc tcggttaccg atgaagagct gaaggcgctg cacgccgccg
      301 gcgtgcgcgg cgcgcgcttc aatttcgtca agcgactggt cgacgccctg ccgcgcgatg
      361 ccctctccga gatcgcggcg cggttggctc cgctgggatg gcacatcgtg atctatttcg
      421 aggcgcagga actgcccgag ctctatgatt tcttcaccag cctgccgggc acggtggtgg
      481 tcgaccatat gggccgtccc gatgtgacga agtcggtgga cgggccggaa ttcggcctgt
      541 tccagaagct gatgcgcgag aaccccaact tctggtcgaa ggtcacgtgt cccgagcgtc
      601 tgtcgaggct ggatccggac ctctacgacg atgtcgttcc ttttgcccgg cgcctggtcg
      661 aggaattccc cgacagagtg ctgtggggca cggattggcc gcaccccaat ctcacgacct
      721 acatgcccga cgacggcaag ctggtcgatt atgtgccgaa gatcgcaccg acggccgact
      781 tgcagcgcca gttgcttgtc gaaaatccga tgcggctcta ctggcccgag gagtgcgcct
      841 gatgactctg aagaaaccgt ggagaaatcg atatgtttga cgcggcgtgc cgttcggagc
      901 cgtgacactt aatccataga gaggttgcga ccgtcggcat tcacggccgc atcaagtcca
      961 acgtcgtcgt ggcttgctcc atccgccggc aggcctcaag ctggtgagat gaaacgcatt
     1021 ctcttcgccg ccctgctatc gaccggtgcc ctcgcgcacg ccgacactgg cgtcgccatc
     1081 cacagcacga cactccgcaa tgacctcggc atggaagtgt cattcctcga ttacggcggc
     1141 gcgataaccc gggtcgtggt gccggaccgc caaggccata tgggcaatgt cgtgctgggg
     1201 ctgccggatg cggaggccta tcgcaagagc accagccgcc acggctcgat cgtcggccgc
     1261 tatgccgggc gcatcgccgg ggcgcagctg gtcatcgatg gcaggaccat ccatctggtg
     1321 cccggtccgc gcgggccgac cctacatagc gatcccgaca gcttcgacca tcgtctgtgg
     1381 caaggccggg atttcgccga caaggaatcc ctcgggcgcg tcctgaccct gcattgcccc
     1441 gatggcgacc agagctatcc gggcaggctc gatgtccaag tcacctatcg cctgatgcgt
     1501 gcctccaacg agttccgcat cgaatacgag gcccgcagca gcgagccgac ggcgatcaat
     1561 ctgaccaacc acgcttattt caatctggcg ggggcggggg ccgtgggcgt gtccagccac
     1621 aaggtgacga ttccagccga tcgctatgcc gcgctcgacg agctgcatat tcccaccggc
     1681 gacctgccca gcgtcgccgg tggcgatctc gattttcgcg atggccgcgt gctgggggac
     1741 aagccttctt atgacgacag cctgttcctc ggcgcgggcg gcgggttgaa gccggcggcg
     1801 ttggtcgagg agcccggcag cggccggcgg ctggagatca gcaccaccga accctccatc
     1861 cagatctata ccgccaacag tttcaacggc acggaaatgg gcgccgaagg gcattgctat
     1921 caccgcaatg acggcgtggc gctggaaacc cagcacctgc cggacagccc ccatcaccct
     1981 gacttcccct cgaccattct gcggccgggg g
//
LOCUS       DATGEM010000219         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_983892, whole genome shotgun sequence.
ACCESSION   DATGEM010000219 DATGEM010000000
VERSION     DATGEM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983892"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VKQ27_06885"
     CDS             <1..830
                     /locus_tag="VKQ27_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HLY88694.1"
                     /translation="IEWVDGVMRYQRTVALDGVQGTVSVEPNDRGNGLRVRIRFPRVG
                     WLATIVSRVRRVFDLSADPRAISEHLSADPLLAPLIARRPGLRVPGAWDGFEVAIRAV
                     LGQQITVTAARKLAAKLVANFGDPLVASDDVRLTHTFPAPAKLAVSDVRSLGMPSARA
                     DALISLAAAAAADPRLFSPSETLEDAIVRLRSLRGIGEWTAQYIAMRALREPDALPAA
                     DIGLMRALAQPGGVRPNAAAVLARAEAWRPWRAYAAQHLWTADASMKEHADAEPIAA"
     gene            808..1362
                     /gene="ogt"
                     /locus_tag="VKQ27_06890"
     CDS             808..1362
                     /gene="ogt"
                     /locus_tag="VKQ27_06890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLY88695.1"
                     /translation="MPSPLQPDALFLDRRPSPIGDMLVVHDLQERLRAIEFHDCEGRL
                     MRLLHIHYGARPLQERRAPAVIRDALAGYFAGDLRAIDRLEVATAGTPFQRDVWAALR
                     TIRPGTTLSYGALAQHIGRPRAVRAVGAANGSNPISVVVPCHRVIGADASLTGYGGGI
                     ERKRWLLAHEGAVFKGGLPLQVAA"
     gene            complement(1461..2378)
                     /gene="ftsY"
                     /locus_tag="VKQ27_06895"
     CDS             complement(1461..2378)
                     /gene="ftsY"
                     /locus_tag="VKQ27_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLY88696.1"
                     /translation="MALGFFSRLKEGLTRSTQKLSEGITAVFRKRRLDDEALEELEEV
                     LIAADLGTDVARRVIASFRRSRFGSEVTDEEIKQTLGEEISAILAPVAQPLQLDPAKK
                     PHVVLVVGVNGVGKTTTIGKLAQNFRDAGKRAVLVAGDTFRAAAVEQLQIWGDRVGAP
                     VVAGGPNADAAGLSFNALTQAKAEGADVLLIDTAGRLHNKSVLMDELRKIIRVLRKQD
                     ETAPHSVLLVLDATTGQNAVQQVRVFKEMVDVTGLVVTKLDGSARGGIVVALAETFGL
                     PVHAVGVGEKAGDLRPFDAGDFARGLVGT"
     gene            complement(2378..>3076)
                     /locus_tag="VKQ27_06900"
     CDS             complement(2378..>3076)
                     /locus_tag="VKQ27_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLY88697.1"
                     /translation="TGVDIASYGSDLPGTPRLGQLARRLLALVPDLQRLRLSSLDPAS
                     IDDDVWRLLAEEPRLMPHLHLSLQAGSDLILKRMKRRHLRDDAAAAIERARALRPGIA
                     IGADVIAGFPTETDATFQETLDFVTGAELPYLHVFPYSERPGTPAARMPAVPITARRE
                     RAARLREAGRATLHRFMLRQVGRAISLLTETENTGHSEHFAPVRLAAPARPGAVTAAR
                     VIGVVDDALLAEAT"
BASE COUNT          484 a          984 c         1052 g          556 t
ORIGIN      
        1 ggatcgagtg ggtcgatggc gtgatgcgct accagcgcac ggttgcgctg gatggcgtgc
       61 agggaactgt cagtgtcgag ccgaatgatc gaggcaatgg cctgcgcgtg cgcatccgct
      121 tcccacgggt tgggtggctt gcgacgatcg tcagtcgcgt gcggcgcgtg ttcgatctca
      181 gcgcggatcc gcgcgcgatc agtgagcatc tgtcggcgga tccattgctt gcgccgctga
      241 ttgcgcgccg gccaggcctc cgcgttcccg gcgcttggga tgggttcgaa gttgcgatcc
      301 gcgcagtgct cggacagcag atcacagtta ccgccgcgcg caagctggcg gcgaagctgg
      361 tcgcgaactt cggcgatccg ctggtggctt cagacgatgt acggctgacg cacacctttc
      421 ctgcgccggc gaagctggcg gtttcggatg tgcggtccct cggcatgccg tcggcgcgcg
      481 cagatgcatt gatttcgctc gctgccgcgg ctgcggcgga tcctcgattg ttcagtccga
      541 gcgagacgct ggaggatgct attgtcaggc tccgctcgct gcgaggcatc ggcgaatgga
      601 cagcgcaata catcgcgatg cgcgcgctgc gggagccgga tgcacttcct gccgccgata
      661 tcggactgat gcgagcgctt gcgcagccgg gcggcgtccg gccgaacgcg gcggctgtgc
      721 tggcgcgcgc cgaggcctgg cggccgtggc gcgcttatgc cgcgcagcat ctctggaccg
      781 cggacgccag catgaaggag catgccgatg ccgagcccat tgcagcctga cgcgttgttt
      841 ctcgatcggc ggccgagtcc cattggcgac atgctggtgg tgcatgatct gcaggagcgg
      901 ctgcgcgcga tcgagttcca tgactgcgag gggcggctga tgcggttgct gcacatccat
      961 tacggtgcgc ggccgttgca ggagcgtcgt gcgcctgcgg taatccgtga tgcgttggcc
     1021 ggctacttcg cgggtgatct gcgcgcgatc gatcgccttg aggtcgcaac ggcaggaacg
     1081 ccgttccaac gcgatgtgtg ggcggcgttg cggaccatcc ggccgggcac gacgctgagc
     1141 tacggagcct tggcgcaaca tatcggccgg ccgagggcgg tgcgcgctgt cggcgccgcc
     1201 aatggctcca atccgatcag tgttgtcgtg ccgtgtcatc gcgtgatcgg ggcggatgcg
     1261 agccttaccg gctatggtgg cggcatcgag cgcaaacggt ggttgcttgc ccatgaaggg
     1321 gcagtgttca agggcgggct tccgttgcaa gtcgcggcat gaaccgcctg tcattgcgac
     1381 ggctgcgagg gcggacgtta gcaatctcgt tcgggattgc ttcattctgc tcgcaatgac
     1441 aatagtatag acagtgcgcg ttaagttccc accaggccgc gcgcgaagtc gcctgcatcg
     1501 aacgggcgca gatcaccggc tttctcgccg acgccgaccg catggaccgg caggccgaac
     1561 gtttccgcga gtgccacaac gatgccgccg cgcgcactgc catcgagctt ggtcacgacg
     1621 aggccggtta cgtccaccat ctccttgaat acacggacct gctgcaccgc gttctggccc
     1681 gtcgtcgcat caagcacgag gaggacagag tgcggtgcag tctcgtcctg cttgcgcagc
     1741 acgcggatta tcttgcgaag ctcgtccatc agcacggatt tgttgtgcag ccggcccgcc
     1801 gtgtcgatga gcagcacgtc ggcgccttcg gccttcgcct gggtcaacgc gttgaacgac
     1861 agccccgcgg catcggcatt ggggcctccg gcgaccacgg gcgcgccgac gcggtccccc
     1921 cagatctgca actgctcgac ggcggccgca cggaacgtgt cacccgcaac cagcacggcg
     1981 cgcttgccgg catcgcgaaa attctgcgcc agcttgccga tcgtggtcgt cttgcccacg
     2041 ccgttgacgc ccaccaccag cacgacatgc ggcttcttcg ccgggtcaag ctggagtggt
     2101 tgggcgaccg gcgccaggat cgccgagatc tcctcgccga gcgtctgctt gatctcttcg
     2161 tcggtgacct cgctgccgaa ccgcgagcgg cggaaactgg cgataacgcg gcgcgcgaca
     2221 tcggtaccga ggtcggcagc gatcagcact tcctcgagtt cctcgagcgc ttcgtcatca
     2281 aggcggcgtt tgcggaagac cgcagtgatg ccttcgctca gcttttgcgt gctgcgcgtc
     2341 aggccttcct tcaggcgcga gaagaaaccg agcgccatca ggtggcctcc gccagcaatg
     2401 catcgtctac cacgccgatc acccgcgccg cggtcacagc gccgggccgc gcgggcgcgg
     2461 cgagcctgac cggagcgaaa tgttcggaat ggccggtatt ctccgtctcg gtcagcagtg
     2521 agatcgcccg gccgacctgc cgaagcatga aacgatgcag cgtcgcgcgt ccggcctcac
     2581 gcagtcgggc cgcgcgctcg cgacgcgcgg ttatcggcac agcgggcata cgcgccgccg
     2641 gggtgccggg ccgctcgcta taggggaaca catgtagata cggcagctct gcgccggtca
     2701 cgaaatccag tgtctcctgg aacgtggcgt cagtttcggt gggaaatcct gcgatcacgt
     2761 cggcaccgat cgcgatgccg ggccgcagtg cacgtgcccg ttcgatcgcc gccgctgcgt
     2821 cgtcgcgcaa gtgccgccgc ttcattcgct tcaggatcag gtcgctgcca gcctgtaggg
     2881 acagatgcag atgcggcatc agacgcggct cttcggcaag caggcgccag acgtcatcat
     2941 cgatcgacgc ggggtccagt gacgacagcc gcaatcgttg caagtcgggc acaagggcaa
     3001 gaagccggcg tgccagttgc cctagccgtg gcgtgcccgg cagatcgctg ccgtaggagg
     3061 cgatgtccac gccggt
//
LOCUS       DATGEM010000220         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_25386, whole genome shotgun sequence.
ACCESSION   DATGEM010000220 DATGEM010000000
VERSION     DATGEM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25386"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /gene="ligD"
                     /locus_tag="VKQ27_06905"
     CDS             <1..1973
                     /gene="ligD"
                     /locus_tag="VKQ27_06905"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HLY88698.1"
                     /translation="GWISEIKFDGYRLLCWKDGGDVRIMTRNGLDWTDRLPAVARTVA
                     RLNAHSAIVDGELVSLDKSGVSSFSALQAALSAGRDEKLHLCLFDLLYLDGWDLRACR
                     LIDRKKALRRLSDWRGKLRYSDHHDGDADKMRRQACEMGLEGIICKQADSSYRSGRGR
                     DWLKVKCRGREELVVLGWTPPGGSRTGLGSLHLGYYDAKGWLHYAGGVGSGFSDDELG
                     RISERLGNLASDPPKSLIAAGDPLPKDIHWVKPRLVAEVQFASWSGAGRVRQAVYLGL
                     RDDKTASEVVREPADPKAERETLHPSAPVNGGKRRPVIAVPPRRDAPKRGAGNGKGGP
                     IVTAQAPAKRGVMVGGVSLSHPGRELWPGITKQDLAEYWAAIADRALPGLAGRPLAIV
                     RCPAGIAGEHFFQKHGHGALPPQVRQGEADGQPYLVIDDAEGLIAMAQISAIELHVWG
                     ATEADPLKPDQIVFDLDPGDDVAFAEVVRAALDVRERLQRVGLEPFCRTTGGKGLHVV
                     VPLKPAAGWDQVKPFCRAFAETLSEEEPKRFLSTVKKADRQGRILIDWLRNGLGATAV
                     ASYCPRARPGAAVAMPMAWDDVTTKLDPAAFTLRNTPQSLARQKRDPWASFDKARQPL
                     PAMAQEAPRTRTRAPSKGSVVIVTAAKPKRRH"
     gene            2263..3096
                     /locus_tag="VKQ27_06910"
     CDS             2263..3096
                     /locus_tag="VKQ27_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY88699.1"
                     /translation="MAAVLPVPTLDHVVVNARDQIDEGLEAYQRIGFTMTPRGYHTLG
                     SMNHLAILGTEYLELIAARPGETRRAEILASPFGLNGLVFGTDDSAAVYSALHEAKVP
                     VEPPLEFSRPVELPDGSTRDATFRTVRLTPATVPAGRIYFCHHFTRDLVWRDEWRHHA
                     NGAIGVIRAVIAARDPSVLGGLFSRMFGSHAVRQMANGCFLAVGLSRFDVLAIEEVAR
                     QFGDAAPDGDGRDEYMAALTLRTRSLDLAAGAIAGGQREANRIVVPASQAFGVTLEFR
                     A"
     gene            3096..4574
                     /locus_tag="VKQ27_06915"
     CDS             3096..4574
                     /locus_tag="VKQ27_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HLY88700.1"
                     /translation="MSANLGGAVASNDPSALALIDLGGGAPRTYSYADIDRLSNACAR
                     GLLRRGLRRGDRVAILATNRAEYLIAFLGTMRAGLVSVPVNHRLPAETVGFIIEDSDS
                     RLVLTDRARTSLLRADVPRVVIDEDFASLLDEGEWTAPEAETAEPAMFLYTSGSTGRP
                     KGVVLSHRSHLWVLGMRATPTPGLRVLVAAPLYHMNALSMSQVTLNNGGTIVLLPGFN
                     AAGFIDAASRYQVNAITGVPTMIAMMLREHDAISTADLSSVQTLRIGSAPLSEQLIAQ
                     VRQAMPNVRILHGYGTTEAGPVAFGAPSADGMDAPPLSVGAPHPAVQVRLMRGRVAVD
                     DEGVLEMRCPAVMNCYHKLPDVTRKAITPDGFYITGDVFRRDTNDFYYFVGRADDMFV
                     CGGENIYPGEVEKMLERHPAVHQACVVPIPDELKAHKPVAFIVARPDTAVGEDEIRQF
                     ALAHAPAYQHPRRVWFLDELPLASTNKIDRKRLMQQAMMEVG"
     gene            4571..5584
                     /locus_tag="VKQ27_06920"
     CDS             4571..5584
                     /locus_tag="VKQ27_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLY88701.1"
                     /translation="MKAVIVREHGGLDVMHYAEDFPDPSIGEGDVLVRVRASSLNYHD
                     VFTRRGMPGIKIPMPLIMGLDVAGEIAAVGHGVSGWSVGDRVLVDPINRVEGGLMGET
                     MHGGLAELCRARAHQLVRIPDDVSFVDAAALPVAYGTAHRMLVSKGNVRAGDKVLILG
                     ASGGVGVCCVQLAKQAGAEVVVCASSSAKLDRLHALGADHLVNYAERDFVQAVYELYG
                     KPSRRGTNIGHGADVVVNFTGGDTWVKSLRCLKVGGRLLTCGATAGYDPKEDIRFIWT
                     FELEVRGSNGWQRSDIEALLQMVREGRLNTLAERTYPLAQAAEALRVIEAREVFGKIV
                     VTP"
BASE COUNT          967 a         1589 c         1972 g         1076 t
ORIGIN      
        1 aaggctggat cagcgagatc aagttcgatg gctatcgcct gctttgctgg aaggacggcg
       61 gcgatgtgcg catcatgacg cgcaatggcc tggactggac cgatcgcctg cccgcggtgg
      121 cgcgcacggt cgcccgtttg aacgcccatt cggcgatcgt cgatggcgag ttggtgtcgc
      181 tggacaagag tggcgtctca agcttctccg cattgcaagc tgcgttgtcc gctgggaggg
      241 atgagaagct gcacctgtgc ctgttcgatc tgctttatct cgatggctgg gatctgcggg
      301 cttgcaggct gatcgatcgc aaaaaggcac tgcggcgcct gtcggactgg cgcggcaagt
      361 tgcgctacag cgatcatcat gatggcgatg ccgacaagat gcggcgccag gcgtgcgaga
      421 tgggattgga aggaatcatc tgcaaacagg ctgattcgtc ctatcgctct ggacgcggcc
      481 gtgactggct gaaagtgaaa tgtcgcgggc gggaagaact tgtggtgctt ggttggactc
      541 caccgggcgg cagccgcact ggtctgggtt cgctccatct gggctactac gatgcgaagg
      601 gatggctgca ttatgccggc ggcgttgggt ccggcttttc ggacgacgaa ttggggcgga
      661 tcagcgaacg tctgggcaac ctggcatccg atccgccgaa gtcgttgatc gctgccggcg
      721 atccgctgcc gaaggacatt cactgggtga agccgcgact ggtagcggaa gtgcagttcg
      781 cctcgtggtc aggtgcggga cgggtgcgcc aggcggtcta tctcgggctg cgggacgaca
      841 agacagcatc ggaggtggtg cgtgagccgg cggacccgaa agcggaacgg gagacgctgc
      901 atccgagcgc gccggtcaat ggtggcaagc ggcgaccagt cattgccgtg ccgcctcggc
      961 gggacgcgcc gaagcggggc gccggcaacg gcaagggcgg gccgatcgtg accgcccagg
     1021 caccggcgaa gcgcggtgtt atggttggcg gcgtttcgct gtcacatccg ggccgcgagt
     1081 tgtggcctgg catcaccaag caggatctcg ccgagtactg ggccgccatt gccgatcgcg
     1141 cgctgcccgg gctggcgggg cgtcccctgg cgattgtgcg atgccctgcg ggtatcgccg
     1201 gggagcattt cttccagaag catgggcatg gcgcgctgcc accgcaggta cggcaaggtg
     1261 aggcagacgg gcagccatac ctggtgatcg acgatgcgga gggattgatc gcgatggcgc
     1321 agatctccgc aatcgagctg cacgtgtggg gcgcgacgga agccgacccg ctgaagccag
     1381 atcagatcgt gttcgacctc gatcccggtg acgacgtggc gttcgccgag gtggtgaggg
     1441 cggcgctcga tgtacgtgaa cggttacagc gcgtgggact tgaacccttc tgtcgcacca
     1501 cgggcggcaa gggacttcat gttgtggtgc cgctgaagcc tgcggccgga tgggaccagg
     1561 taaagccgtt ctgccgtgcg ttcgctgaga ccctgagcga ggaggagccg aagcgcttcc
     1621 tttccactgt gaagaaggcg gatcgccagg gccggatcct gatcgactgg ctgcgcaatg
     1681 ggttgggcgc aacagcagtc gcatcgtatt gcccgcgtgc acggccgggg gccgcggtgg
     1741 caatgccgat ggcatgggat gatgtgacga cgaagcttga tccagctgcg ttcacattgc
     1801 gtaacacgcc gcaaagcctg gcaagacaga agcgtgatcc gtgggccagc ttcgacaagg
     1861 cacgacagcc gctgccggcg atggcacaag aggcgccccg aaccaggacg cgtgcgccat
     1921 ccaagggaag cgtcgtgatc gtgaccgcgg cgaagccgaa gcggcggcat tgaaggatta
     1981 tgacgctttt gtcacttcca tgcatcggcg tctgttaggc aagcttataa cgactcgatc
     2041 agtttattgc gtttttttca tcgtgaactt tatcgagcca taattcagcg gcgcgcgtaa
     2101 ctgtgagctt gacatcggta gcagtgcgcc aatgtgctgg gagcggcaga tggcctccgc
     2161 catctcgctg cgtgacgttt cccccccaaa cttggcggcg cccttcggga cgccgccttt
     2221 ttgttttacg gtgctcgccg gtcgcagtta gggagcaaca ggatggcggc ggttttgccc
     2281 gtgccgacgt tggatcacgt cgtggtcaac gcgcgcgatc agatcgacga ggggctcgaa
     2341 gcctatcagc gcattggatt cacgatgacg ccacggggct atcacactct cggctcgatg
     2401 aaccaccttg cgatccttgg gaccgagtac ctggagctga tcgccgcccg gccgggtgag
     2461 acccggcgcg ccgagatcct ggcttcgccg tttgggttga acgggctggt tttcggcaca
     2521 gacgactctg ccgcggtgta ttccgcattg catgaggcga aggtgccggt ggaaccgccg
     2581 ctggaatttt cccggccggt cgaactgccg gacggcagca cacgcgatgc gactttccgc
     2641 acggtgcggc tgacgccggc gacggtgccg gcaggacgta tctacttctg tcatcacttc
     2701 acacgcgacc tggtgtggcg cgatgagtgg cgtcatcacg ccaatggtgc gatcggggtg
     2761 atccgggcgg tgatcgcggc gcgtgatcct tccgtgctgg gcgggctgtt ctcgcgcatg
     2821 ttcggcagcc atgcagttcg gcagatggcg aatggctgtt tccttgccgt gggactgtcg
     2881 cgcttcgatg tgctggcgat cgaggaggtg gcgcgccagt tcggcgatgc cgcaccggac
     2941 ggcgatgggc gcgacgagta catggcggca ttgacgctgc gcacgcgctc gttggatttg
     3001 gcggcaggcg cgatcgctgg cgggcagcgt gaagcgaacc gcatagttgt gccggcgtcg
     3061 caggcattcg gcgtcacact ggaattccgc gcgtgatgtc tgcgaatctc ggcggtgctg
     3121 ttgcctcgaa cgatccatcg gcgcttgcgc tgatcgatct tggtgggggt gcgccgcgca
     3181 cttacagcta tgcggacatc gaccggctca gcaatgcatg cgcgcggggt ctgctgcggc
     3241 gtgggctgcg gcgcggcgat cgcgtggcga ttctggcgac caaccgcgcc gagtatctga
     3301 ttgcgttcct ggggactatg cgcgccgggc tggtatcggt gccggtgaac cacagactgc
     3361 ccgctgagac ggtcggtttc atcatcgagg actccgactc ccgccttgtg ctgaccgaca
     3421 gggcgcgcac atcgctgttg cgcgccgatg tgcctcgggt ggtgatcgat gaggacttcg
     3481 cgtcgctgtt ggatgaaggc gaatggacgg caccggaagc cgagacggcc gaaccggcga
     3541 tgttcctgta tacatcgggg tcgaccgggc ggccgaaagg cgtggtgctg tcgcaccgga
     3601 gccacctctg ggtgctgggg atgcgtgcga cgccgacgcc gggactgcgc gttctggtgg
     3661 ccgcaccgtt gtaccacatg aacgcgctgt cgatgtcgca ggtgacactg aacaatggcg
     3721 gcacgatcgt gctgctgccc ggcttcaatg cagcaggctt tattgatgct gcatcacgct
     3781 atcaggtgaa tgcgataaca ggtgtgccga cgatgatcgc gatgatgctc cgcgagcacg
     3841 atgcgatatc gacggctgat ctctcatccg tgcagacact gcgcatcggg tcggcgccgc
     3901 tgtccgaaca gttgatcgcg caggtgcgcc aggcaatgcc gaatgtgcgc attttgcacg
     3961 gttatggcac gaccgaagct ggtccggtgg cgttcggtgc gccgtccgcc gatggaatgg
     4021 atgcgccgcc gctatcggtt ggtgcgccgc atccggccgt gcaggtgcgt ttgatgcgcg
     4081 gtcgcgtcgc ggtcgatgat gaaggcgtgc tggagatgcg atgcccggcg gtgatgaact
     4141 gctatcacaa gctgcccgac gtcacgcgca aggccatcac gccagacggc ttctatatca
     4201 cgggcgacgt gtttcgccgc gatacgaacg acttttacta cttcgtcggc cgcgccgacg
     4261 atatgttcgt ttgcggcggc gaaaacatct atcccggcga agtggagaag atgctggaaa
     4321 ggcaccctgc cgtgcatcag gcctgcgtcg tgccaatccc cgacgagctg aaggcgcaca
     4381 aaccggtggc gttcatcgta gcgcgccccg acaccgcagt gggtgaggat gagatcaggc
     4441 aatttgccct ggcgcacgcg ccggcttacc agcaccctcg gcgagtctgg ttcctggacg
     4501 aactgccgct cgccagcacc aacaagatcg atcgcaagcg gctcatgcag caggccatga
     4561 tggaggtcgg atgaaagcgg ttattgtccg cgaacacggc ggactcgatg tgatgcacta
     4621 cgccgaggac ttcccggatc cctcgattgg cgaaggggac gtgctggtgc gcgtgcgagc
     4681 gtcatcgctg aactaccacg acgtgttcac gcgccgcggg atgccaggca tcaaaattcc
     4741 gatgcctctg atcatggggt tggatgtcgc gggagagatt gccgcagtgg gacacggcgt
     4801 cagcggatgg tcggtcggcg atcgcgttct ggtggatccg atcaaccgcg tggaaggcgg
     4861 attgatgggc gagaccatgc atggcggcct cgccgagcta tgccgcgcac gggctcacca
     4921 attggtacgg attccggatg atgtcagttt cgtcgatgcg gcagcgctgc cggtcgcgta
     4981 cgggacggcg catcggatgc tggtgtcgaa gggcaatgtg cgtgccggcg acaaggtgtt
     5041 gatccttggc gccagcggcg gcgtcggagt ctgttgcgtg cagcttgcca agcaggctgg
     5101 tgcagaagtt gtggtgtgcg ccagcagttc cgccaagctc gatcggcttc atgcgctggg
     5161 ggcggatcat ctggtcaact acgccgagcg cgacttcgtg caggcggtct acgaactgta
     5221 cggcaagccg tcgcgtcgtg gcaccaatat cggccatggc gccgacgtgg tggtgaattt
     5281 caccggcggg gatacatggg tgaagtcgtt gcgctgcctg aaggtgggcg ggcggctgct
     5341 gacctgtggc gcgaccgcgg gctacgatcc gaaagaggat atccgcttca tctggacgtt
     5401 cgagctggaa gtccgcggtt ccaacggctg gcagcgcagc gatatcgagg cgctgttgca
     5461 gatggtacgc gaagggcgtt tgaacacgct ggcggagcgc acctatcctc tggcacaggc
     5521 ggctgaggca ctgcgcgtga tcgaagctcg cgaggtcttc ggcaagatcg tggtcacacc
     5581 atgaccgagc cgctgaccaa ggaa
//
LOCUS       DATGEM010000221         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_624646, whole genome shotgun sequence.
ACCESSION   DATGEM010000221 DATGEM010000000
VERSION     DATGEM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624646"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VKQ27_06925"
     CDS             <1..888
                     /locus_tag="VKQ27_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLY88702.1"
                     /translation="YHVASGMFDVVLTAGADKIGECGDSQTILNKIWDPSYERPLPLG
                     TINMLAMSAVRYMQRYGMTEEDMARVTVKNRRHASLNPKAHLRRIVSVDDVLASRLIS
                     WPIKLMDCCPQSSGGAAMVLASEKFIRHNHLDAVWITGVGHVAETYWMGDRMGNNPTA
                     EHADAYALGRSFEKSYRMAGITDPARQVHVAELYAPFSNTEFHSIDAARLVSRPGESV
                     ARLRDGEFDIGGKIPVNPSGGTLCTNAIAVTAMARVAEVALQVWGRAGDHQVKGAQLG
                     IASGNGGDHQIFGTMVIEA"
     gene            898..1353
                     /locus_tag="VKQ27_06930"
     CDS             898..1353
                     /locus_tag="VKQ27_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY88703.1"
                     /translation="MELLERQEVWNITYHHALGETASWFYTRIRDEAAIYGRRCSKTG
                     RVLVPPRSFSDETLLPTTDWVKVGPGGRIEAFTIVYEKFNNLPDPPYAFGYVLLDGAD
                     TALGGYFKGIDLSDPQAAAAKLKIGTRVAATFAPERKGEMLDFWFELAA"
     gene            complement(1433..2293)
                     /locus_tag="VKQ27_06935"
     CDS             complement(1433..2293)
                     /locus_tag="VKQ27_06935"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY88704.1"
                     /translation="MSVRPLHPALGAEVRGVDMRTPPDPETLRAVHDAWMEYLVLVFP
                     GQQITDEEHVAFTRGLGEPEIFHQNIIRSRRVKEIFRVSNVDDEGNLMPPDHPTVKQV
                     SLAQFWHTDSSYRTIPCTGALLHGVETSRTGGETQFTNMYAVYEALPESLKRQVQGRK
                     AQHNFGHLHTLANLKPLTEEEIAAMPPVWQPMVRRHPVTGRHSLYISPIYNDEVEGMD
                     PADAKHLIADLAEFAAEPRFVYRHRWEPHDVVMWDNRCTMHQVTPHDPRERRVMHRTT
                     IVGTEPVLAA"
     gene            complement(2348..3304)
                     /locus_tag="VKQ27_06940"
     CDS             complement(2348..3304)
                     /locus_tag="VKQ27_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HLY88705.1"
                     /translation="MPGDDRPRSIAEMPAQRPAAPATPAEARGRFFNTGNGFNVQLPA
                     VPDQIFTREPAIALDPATPTGLIACDIAADLASGFPATTPLVLARYARVRAGEHLTTD
                     FVASGVIGYVIAGSGRTSCAGQNIAWSAGDLFILPGGEPALHSADDIDAVLWLVTNEP
                     QLAFESLQAPSPGKAPTDVVHYRADEITRQIELIYDIGRNEETAGSALIFSAEQQEAT
                     RNVLPTLTVAMNSLPGGAVQRPHRHNSVAVSLIIKGDRCFSIIDGRRKDWAPWATTIT
                     PPVAVHSHHNGGNEQALFLIVQDGGIYYHTRAMGFEFAEHPA"
     gene            complement(3399..4313)
                     /locus_tag="VKQ27_06945"
     CDS             complement(3399..4313)
                     /locus_tag="VKQ27_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY88706.1"
                     /translation="MTGIMEGKVVIVTGAGGGIGREIAIMMAAAGARVIINDVGASLT
                     GSGASITPAQQTQAIIRQNGSLAEISTDSVAEWASAQKIIQSALDHFGRIDAVVNNAG
                     ILRDGIFHRMSADDWLAVISVHLNGSFFVSRAAAEHYRKQESGTFVHITSTSGLIGNV
                     GQANYAAAKLGITALSKSIALDMQRYNVRSNCLSPWAWSRMTSSIPATTPEQQVMVEK
                     LQRMTPDKNAPLAVFLASDAAMGVTGQVFGARMNEIYLFSSPRPIRIVHRADGWTPET
                     IAQHALPAMRAAFMPLDTSEQAFPWEPV"
BASE COUNT          779 a         1362 c         1488 g          869 t
ORIGIN      
        1 tatcacgttg cctccggcat gttcgacgtt gtgctgaccg cgggcgccga caagatcggc
       61 gaatgcggag attcacagac gatcctgaac aagatctggg atccgagtta cgaacgtccg
      121 ttgccgctcg gcaccatcaa catgctggca atgtccgcgg tgcgctacat gcagcgctac
      181 ggcatgaccg aggaggacat ggcacgtgtc acggtgaaga accggcggca tgcgtcactg
      241 aacccgaagg cgcatctgcg gcggattgtc tcggtcgacg atgtgttggc gtcgcggttg
      301 atatcctggc cgatcaaact gatggattgc tgtccgcagt catcgggcgg tgccgcgatg
      361 gtgttggcct cggaaaagtt catccgccac aatcatctgg atgcggtgtg gataaccggc
      421 gtcggccatg tggccgagac gtactggatg ggcgaccgca tgggcaacaa tccgaccgcc
      481 gagcatgcgg acgcctatgc gctggggcgt tcgttcgaga agtcgtaccg gatggcgggt
      541 atcacggacc cggctcgaca ggtgcacgtg gcggagctgt acgcgccgtt cagcaacacc
      601 gagttccact ccatcgacgc ggcgcgcctg gtgtcgcgcc cgggcgagtc agtggcgagg
      661 ttgcgcgacg gggaattcga tatcggcggc aagatcccgg tcaatccatc cggcggcacg
      721 ctctgcacca acgccattgc ggtgacggcg atggcgcgcg tcgccgaggt ggcgctgcag
      781 gtgtggggac gcgcaggcga ccatcaggtg aagggcgcac aactgggcat cgccagcggc
      841 aacggcggtg accatcagat ctttgggacg atggtgatcg aggcatagga gcgagagatg
      901 gaactgctgg aacgtcagga agtctggaat atcacctacc atcacgcgtt gggcgagacc
      961 gcgagctggt tctatacgcg cattcgtgac gaagccgcga tctacggccg gcgctgtagc
     1021 aagaccggtc gcgtgctggt gccgccgcgg tcgttctcgg acgaaacgct gctgccgacg
     1081 acggattggg tgaaggtggg accaggcgga cgcatcgagg cgttcaccat tgtctatgag
     1141 aagttcaaca atctgcctga tccgccctat gccttcggct atgtcttgct ggacggcgcc
     1201 gatactgcac tgggcggcta tttcaagggc atcgacctgt ccgatccgca ggcggcagct
     1261 gcgaagctga agatcggcac gcgcgtcgct gcgaccttcg cgccggagcg caagggcgag
     1321 atgctggact tctggttcga gctcgccgcc tagatgtcga agctgtagag gttgtccagc
     1381 cacgcgaggt tcgggttcgg atgctcgacg acaagtggtt gtgtggccga catcaggcgg
     1441 ccaggacagg ttcggtgccg acgatcgtcg tgcgatgcat cacgcggcgc tcgcgcggat
     1501 cgtgcggcgt cacctgatgc atggtgcatc ggttgtccca catcaccacg tcatgcggct
     1561 cccagcggtg gcgatagaca aagcgcggct ccgcggcgaa ttccgccaga tcagcgatca
     1621 ggtgcttggc atccgcgggg tccatgccct cgacttcgtc attgtagatt gggctgatat
     1681 agagcgaatg acgcccggtg accggatgcc tgcgcaccat cggctgccag accggcggca
     1741 tcgctgctat ctcttcctcg gtaagcggct tcagatttgc cagcgtgtgc aggtggccga
     1801 aattgtgctg cgccttgcgg ccctgcacct gccgcttcag agattcgggg agtgcctcgt
     1861 acacggcgta catgttggtg aactgggtct cgccaccggt gcgactggtt tcaactccgt
     1921 gcagcagcgc gccggtacag ggaatggtgc gatagctgct gtcggtgtgc cagaattgcg
     1981 ccagtgatac ctgcttcacc gtcggatgat ccggcggcat caggttgccc tcgtcatcca
     2041 cattggagac ccggaagatc tctttcacgc ggcgcgatcg gatgatgttc tgatgaaaga
     2101 tctccggctc gccgaggccg cgcgtgaagg cgacgtgttc ctcgtcggtg atctgctgcc
     2161 cagggaacac cagcaccagg tactccatcc atgcatcatg cacggcgcgc agggtctcgg
     2221 gatcgggtgg cgtgcgcata tccacgccgc gcacctccgc gccgagcgcc ggatgcagag
     2281 gacgcacgct cacttgggct gaattggttg ctgccgctga tgccatggct gctgcctttc
     2341 cgttgtctca ggccgggtgt tcggcgaact cgaagcccat tgcacgcgtg tggtagtaga
     2401 tcccgccatc ctgcacgatc aggaacagcg cttgctcgtt gccgccgttg tggtgggaat
     2461 gaaccgcgac gggtggcgtg atcgtcgtcg cccacggcgc ccagtccttg cgcctgccgt
     2521 caatgatcga gaagcagcgg tcgcccttga tgatgagcga cacggcgacc gagttgtgac
     2581 ggtgcggacg ctgcaccgcc ccacccggca gcgagttcat cgcgaccgtg agcgtcggca
     2641 ggacgttgcg cgttgcttcc tgctgctcgg cggagaagat cagcgccgag cccgcggtct
     2701 cttcgttgcg gccgatgtcg tagatgagct cgatctgccg cgtgatctcg tcggcccgat
     2761 agtgcacaac gtcggtcggt gccttgccgg gcgacggtgc ctgcaggctc tcgaacgcaa
     2821 gttgcggttc gttggtcacc agccacagca ctgcgtcgat gtcatccgca ctgtgcagcg
     2881 ccggttcgcc gccaggcagg atgaacaggt cgccggcgct ccacgcgata ttctgtccag
     2941 cgcacgatgt acgaccgctg ccggcgatga cgtagccaat gacgccactc gccacgaagt
     3001 ctgttgtcag gtgttcgccg gcacgaacac gcgcgtagcg cgccaacacc agcggcgtgg
     3061 tcgcgggaaa accgctcgcc aggtcggcgg cgatgtcaca cgcgatcagc cccgtcggcg
     3121 ttgccgggtc cagggcgata gccggttcgc gggtgaagat ctggtccggc acagcgggca
     3181 attgtacgtt gaacccgttc ccggtgttga agaaccggcc gcgcgcctcg gccggcgttg
     3241 ccggcgcggc agggcgttgc gccggcatct cggcaatgct gcgtggacga tcgtctcccg
     3301 gcatcgatgg ctctcctcgt ggcaccgact tgtgccacca tcgcgggtgg atggcagcat
     3361 gtcaagattc ccgaataatg ttcggttatg gacggtattc agaccggctc ccagggaaac
     3421 gcctgctcgg atgtatcgag tggcatgaat gctgcccgca tcgccggcaa cgcatgctgc
     3481 gctatcgtct cgggcgtcca gccatcggca cgatgcacga tgcggatcgg gcgtggcgag
     3541 ctgaacaggt agatctcgtt catgcgcgcg ccgaacacct ggccggtgac acccatcgcc
     3601 gcatccgatg cgaggaacac cgcgagcggc gcattcttgt ctggcgtcat gcgctgcagc
     3661 ttttccacca tcacctgctg ttccggcgtg gtcgccggaa tcgaactggt catgcggctc
     3721 caggcccacg gcgagaggca attggaacgg acgttgtagc gctgcatgtc gagcgcaatc
     3781 gacttcgaca gcgcggtgat gccgagtttg gccgcggcat agttggcctg gccgacgttg
     3841 ccgatcaggc cggacgtgct ggtgatatgt acgaaagtgc cgctttcctg cttgcggtaa
     3901 tgttctgctg cggcacggct gacaaagaag ctgccgttca ggtgcaccga gatgacggcg
     3961 agccagtcat cggcgctcat ccgatgaaag atgccgtcgc gcaggatgcc ggcgttgttc
     4021 accaccgcgt cgatgcggcc gaagtgatcc agcgcagact ggatgatctt ctgcgccgag
     4081 gcccattccg caacggagtc ggtactgatc tcggccaggc tgccgttctg gcgaatgatc
     4141 gcctgggtct gctgtgcagg cgtgatggac gcgccgctac cggtgagcga tgcgccgaca
     4201 tcgttgatga tcaccctggc gccggcggcc gccatcatga tggcgatctc gcgcccgatg
     4261 ccgccgccag cgccggtgac gatgacgact ttgccttcca tgatgcctgt cacggccact
     4321 ctccttgttc ggtgcggtac ggcagattga cagccagggg cggctctcgc aacgtcaatc
     4381 ctgaatagaa ttcgggtttc taccgatgct tggcctcaat ctcgactatc cgctggtgct
     4441 gtccaccatc atcgagcacg ccgcagcaac gttcggggac acggagctcc tatcgcac
//
LOCUS       DATGEM010000222         5303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1515807, whole genome shotgun sequence.
ACCESSION   DATGEM010000222 DATGEM010000000
VERSION     DATGEM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515807"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VKQ27_06950"
     CDS             complement(<1..1047)
                     /locus_tag="VKQ27_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY88707.1"
                     /translation="MHDRSDIAHETARRRFLGGMAFGAATLAASRALAAPSGDAHAAG
                     YDVSPASNVMQTVPRKPGDAAAFTFRLDTNAVKATTGGWAREVTARSLPLATDLAGAH
                     LYMNPGASREMHWHTSAEWAFILGGQCQIAVLDPAGELDVANYGPGDLWYFPKGHAHA
                     IFTLGNEPCHALLVFNDGLYSERGTFGLSDWLSRYDAATLARNFGVQPASFASFPQGE
                     TYINQGAVIPLDSVAARGVHELPQARSHRYRLMASKPWRSFPGGTLHLAAAHEFPIAD
                     ITALIIRLRPGGMQELHWHPNANEWFYVSKGHVRATLFTADKHMAVAELSAGDCGYFP
                     RGWGHSLEALGGDGC"
     gene            1294..2337
                     /locus_tag="VKQ27_06955"
     CDS             1294..2337
                     /locus_tag="VKQ27_06955"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLY88708.1"
                     /translation="MFIRNAWYVAAWADEVSETPLARRICNEPVVLYRDQQGHAAALI
                     DMCCHRGAPLSVGKVVEQGLQCGYHGLIFDRSGTCVHVPGQDRIIERTRVRSFPVVEQ
                     DAFLWIWMGDPAKADPATIVPWPYHNDAVNWPHKHTMYPIKASAMLMVDNLMDLTHLG
                     YIHVSTIGGNPTQHVDAKMETERTSLGVRFTRWMLNSVPPPTYVKAVGFQGRIDRCQR
                     FEFVAPGSVLQWTGATEAGGYRDGDTSDSKLEFRLYHGLTPETDTSCFYFWSAANGFR
                     RDEPEATEQFFQQVAAAFLEDKTVVEAQQTRLSELGEAALVDIVSDSVRLHMRRLVDQ
                     LIAEEAQPALAAE"
     gene            2411..4150
                     /locus_tag="VKQ27_06960"
     CDS             2411..4150
                     /locus_tag="VKQ27_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HLY88709.1"
                     /translation="MADTHRMPTHHPRIIATRHMVSSANWLAAQAGFEILEAGGNAID
                     AGVAAGIALGVLQCEYVHFAGVAPIMIHLAESSETVTISGLGPWPKLASAAWFREHQD
                     GRILSNIKRTVVPAAPDAWITALERYGTMSFAEVAAAAIRFGREGFGFQSISHEVMAE
                     AADTIRRYPQNAAIYMPNGDVPKPGERFLQTDLANSIQYMADQETAARKSGRAAGLAA
                     ARDAFYRGDIAQKMVAYHKANDGWLREDDLSQFRSDVEQALSARYADVTVFGCGPWCQ
                     GPVLLQTVKILDGLDLRSLGHNTPHYVHTLVEALKLAFSDRHTYYGDPKFVDVPIEAL
                     LSDAYAARRRALIDPERAYPEMPPAGTATELGVAAHPAAPAKADREYVEGDPDTSYVC
                     VVDRHGNCFSATPSDGAINTPVIPGTGVAPSSRGMQSWTDPAHPACLAPGKRPRLTPN
                     PAIVRRDGKWIMPFGSPGNDVQPQAMLQVLLNMVVFDMTPQEAIEQPRFASSSFPSSS
                     DPHSYTPRRMSLERRFSDETAASLDRMGHSVSWWREWEWKAGCVCAIVKDEQTGMMEG
                     GADVRRPGGVRGW"
     gene            4144..4869
                     /locus_tag="VKQ27_06965"
     CDS             4144..4869
                     /locus_tag="VKQ27_06965"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF014650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HLY88710.1"
                     /translation="MVRFACAALLFLFAIRPAAAEDPDALWKIISQQCVPNEREYGEP
                     KPCAKVDLTGEYVVLKDRVGATQFLVLPTDRITGIEDPAILAPKAPNFWEDAWQARSY
                     VDERAHRTMPRDTISLAVNSVSGRTQDQLHIHVDCVRPDVKNALHDHADAIGATWSKF
                     PWQLAGHDYMAMRIDAPDLMHSNPFDMLADDLPGAKSDMGHYTLVVVGQANGFMLLAG
                     HATGLTDHGSGEELQDHTCALAR"
     gene            4885..>5303
                     /locus_tag="VKQ27_06970"
     CDS             4885..>5303
                     /locus_tag="VKQ27_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88711.1"
                     /translation="MQLGFNLPISGPMSHPHCFARLAQAGEAMGYDYLTLTDHVVLPD
                     LSVPGYPYSESGEFIANAPTERHELLTAAAFIAAKTSHIRLVLAVLVVPHRPAVLAAK
                     MLSTIDVLSEGRLVVGVGAGWLKAEFDAVVTTPFAERG"
BASE COUNT          987 a         1728 c         1677 g          911 t
ORIGIN      
        1 gcacccgtcg ccgccgaggg cctccagcga gtggccccag ccgcgcggga aatagccgca
       61 atcgccggca gacagttccg ccaccgccat gtgcttatct gcggtgaaca gcgtcgcgcg
      121 cacatgacct ttcgacacgt agaaccactc gttggcattc gggtgccagt gcagctcctg
      181 catgcctcct ggtcgcagcc ggatgatcaa agcggtgatg tcggcgatcg ggaattcgtg
      241 cgcggcggca agatgcaatg tgccaccagg aaagctgcgc cagggcttgc tcgccatcag
      301 ccgatagcga tggctccgcg cttgcggcaa ttcatgcacg ccgcgtgcag ccacgctatc
      361 aagcggaatc acggcacctt ggtttatgta ggtttcaccc tgcgggaagc tggcgaagct
      421 ggcgggctgc acgccgaaat tgcgcgccag cgtggccgca tcgtagcggc tcaaccagtc
      481 gctgaggcca aaggtgccac gttcggaata caggccatcg ttgaatacca gaagggcatg
      541 acacggctcg ttgcccaagg tgaagatggc gtgcgcatgg cccttgggaa aataccacag
      601 atcgccaggt ccgtagttgg cgacatcgag ttcgcccgcg gggtccagca ccgcgatctg
      661 gcactggccg cccagaatga acgcccattc ggccgatgtg tgccagtgca tttcccgcga
      721 ggcgccgggg ttcatataga gatgtgcgcc ggcaagatcg gtcgcgagcg gaaggctgcg
      781 cgcggtaacc tcgcgtgccc agccaccggt tgttgccttc accgcattgg tgtcgagtct
      841 gaaggtgaac gcagcggcgt cacccggttt gcgcggtacc gtttgcatga cgttgctggc
      901 aggcgatacg tcgtagccgg cagcatgtgc atcgccgctt ggagccgcca aggcgcgact
      961 cgccgcaagc gtcgcggcgc cgaacgccat gccgcccaga aagcggcggc gcgcggtctc
     1021 gtgcgcgata tcagatctat cgtgcatgat tgcagtcccc tgtatccggc cgctaagtcc
     1081 cgccgcgtgc agggacgctg ttgacggccc ccaattacgt tgccatacca atacgggtcc
     1141 cggttgcgct gcgcaacgtt cttcaacaaa acgtgattga atcagaaccg catcgaaagg
     1201 ccgggccagt tggcttttcg tatgaccggc atgcttgacg tcgccggcgg cagcgggatg
     1261 tcatcgctgc aaccaagaca agggagcgcg ccaatgttca tccgcaacgc atggtacgtc
     1321 gccgcctggg cagacgaggt cagcgaaaca ccgcttgctc gtcgcatctg caacgaaccg
     1381 gtcgtgctct accgcgacca acaagggcat gccgccgctc tcatcgacat gtgctgtcat
     1441 cgcggcgcgc cgctcagtgt cggcaaggtc gtcgagcaag gtctgcaatg cggttaccac
     1501 ggcctgatct ttgaccgctc cggcacctgc gtgcatgtcc cgggccagga ccgcatcatc
     1561 gagcgcacac gggtgcgcag cttcccggtg gtggagcagg acgcattcct ctggatctgg
     1621 atgggcgatc cggcaaaagc ggatcccgcc accatcgtcc cctggccgta tcacaacgat
     1681 gcagtgaact ggccgcacaa acacacgatg tatccgatca aggcctccgc catgctgatg
     1741 gtggacaacc tcatggatct gacgcatctc ggctacatcc acgtctccac catcggtggc
     1801 aatcccacgc agcatgtcga tgcgaagatg gagaccgaac gcacgtcgct cggcgtcagg
     1861 ttcacccgct ggatgctgaa ctccgtgccg ccgcccacct atgtgaaagc cgttggcttc
     1921 cagggccgga tcgatcgttg ccagcgtttc gagttcgtcg cacccggatc ggtgctgcaa
     1981 tggaccggcg ccaccgaggc cggtggctat cgcgacggcg acacatccga cagcaagctg
     2041 gaattccgcc tgtatcacgg actgaccccg gagacggaca catcgtgttt ctacttctgg
     2101 tcggctgcga atggtttccg tcgggatgaa ccggaggcga ccgagcagtt cttccaacag
     2161 gtcgccgctg ccttcctgga ggacaagacg gtggtcgagg cgcagcagac tcggctgagc
     2221 gaacttggcg aagcagccct ggtcgatatc gtcagcgact cggtccgcct gcacatgcgg
     2281 cgcctggtcg accaactgat tgccgaggag gcgcagcccg cattggccgc ggagtaggcg
     2341 ccggcaagcc cggcccacgg ttcaacattt catcgcgccg gcgtagcctc tcctccgagg
     2401 gagaaccgac atggccgaca cgcatcgcat gcccacgcac catccgcgca tcatcgccac
     2461 gcgccacatg gtatcttcgg ccaactggct cgcggctcag gctggcttcg aaatcctgga
     2521 ggccggcggc aacgcgatcg atgcaggtgt ggccgctggc atcgcgctcg gcgtcctgca
     2581 atgcgaatac gtgcacttcg caggcgtcgc gccgatcatg atccatctgg ccgagagcag
     2641 cgagacggtg acgatcagcg gcctcgggcc atggccgaag ctggcaagcg ccgcgtggtt
     2701 tcgcgaacat caggacggcc gcatcctctc gaacatcaag cgtaccgtgg tgccggcagc
     2761 accagatgcg tggatcaccg cgttggaacg ctacggcacc atgagcttcg ccgaggtcgc
     2821 cgccgcggcg atccgcttcg gccgcgaggg tttcggcttc cagtcgatct cgcatgaagt
     2881 catggccgag gcggcggata cgatccgccg ctatccgcag aacgccgcga tttatatgcc
     2941 gaacggcgac gtgccaaagc cgggagagcg cttcctccag actgatctcg ccaactcgat
     3001 ccagtacatg gcggatcagg aaaccgcggc acgcaagagc ggacgtgccg ccggactggc
     3061 cgcggcgcgc gatgcattct accgcggcga tatcgcgcaa aagatggtcg cctatcacaa
     3121 ggcaaacgac ggctggctcc gcgaggacga cctgtcgcaa ttccgttccg acgtggagca
     3181 ggccctgtcc gcgcgctatg ccgacgtcac cgtgttcggc tgcggtccgt ggtgccaggg
     3241 gccggtgctg ttgcagacag tgaagatcct ggacgggctg gatctgcggt cgctcggcca
     3301 caacacgccg cactacgtac atacacttgt cgaggcactg aagttggcct tctccgaccg
     3361 gcacacttac tacggtgatc cgaaattcgt cgatgtgccg atcgaggcgc tgctgagcga
     3421 tgcctatgcc gcgcggcgac gcgcgctgat cgatccggaa cgcgcgtatc ccgaaatgcc
     3481 gccagcaggc accgcgacgg agcttggcgt tgccgcgcat ccggctgcgc ccgcgaaagc
     3541 cgatcgcgaa tacgtcgagg gcgatcccga tacctcctat gtctgcgtgg tggatcggca
     3601 cggcaactgc ttctccgcca cgccgagtga cggcgcgatc aacacgccgg tgatcccagg
     3661 caccggcgtc gcaccctcgt cgcgcggcat gcagtcctgg accgatccgg cgcatccagc
     3721 gtgcctcgcg ccaggcaagc ggcctcggct gacaccaaac ccagccatcg tcaggcgcga
     3781 cggcaaatgg atcatgccgt tcggttcgcc tggcaatgac gtgcagccgc aggcgatgtt
     3841 gcaggtgctg ctcaacatgg tggtgttcga catgacgccg caggaggcga tcgaacagcc
     3901 gcggttcgct tcgtccagtt tcccaagttc gtccgatccg cacagctaca cgccgcgccg
     3961 catgagcctg gaacgccggt tcagcgacga gactgcggcc tcgctcgacc gcatgggcca
     4021 tagcgtaagc tggtggcgcg agtgggaatg gaaggcgggc tgtgtctgcg ccatcgtcaa
     4081 agacgagcag accggcatga tggaaggtgg cgccgatgtc cgccgcccag gcggcgtgcg
     4141 cggatggtaa ggttcgcctg cgccgcgctg ctgttcctgt ttgcgatccg ccccgcagcc
     4201 gccgaggacc ccgacgccct gtggaagatc atcagccagc aatgcgtgcc gaacgagcgc
     4261 gagtacggtg aacccaagcc gtgcgcgaag gtcgatctca ccggtgagta cgtcgtactg
     4321 aaggaccggg ttggcgcaac gcagttcctc gtcctgccga cagaccgcat caccgggatc
     4381 gaggaccctg cgatcctggc ccccaaggcg cccaacttct gggaggacgc atggcaggca
     4441 cgcagctacg tcgatgagcg cgcccaccgc acgatgccgc gtgatacgat cagcctggcg
     4501 gtcaactcgg tcagcggccg gacccaggac cagctgcata tacacgtcga ctgcgttcgg
     4561 cccgatgtga agaacgcgct gcacgatcat gccgacgcga tcggcgccac ctggtcgaag
     4621 ttcccatggc aactcgcagg ccacgactac atggcgatgc gcatcgacgc gccggacctg
     4681 atgcacagca atccgttcga catgctcgcc gatgacctgc ccggagcaaa gagcgacatg
     4741 ggccactaca cactggtggt ggtcggccag gccaatggtt tcatgctgct ggccggtcat
     4801 gccaccggcc tgaccgatca tggttcgggc gaggaattgc aggatcacac ctgcgccctg
     4861 gcacgctaat ttcgagggaa cgtcatgcaa cttggcttca atctgccgat cagcggccca
     4921 atgtcgcacc cgcactgctt cgcccgtctc gcgcaggcag gcgaggcaat ggggtacgac
     4981 tacctgaccc tgacggacca cgtggtactg ccggacctca gcgtccccgg ctatccgtat
     5041 tccgagtcgg gcgagttcat cgccaacgca ccgaccgagc ggcacgaact gctgaccgca
     5101 gcggcattca tcgccgccaa gacgtcgcac atccggctgg tacttgcggt gctggtggtg
     5161 ccgcatcgtc ctgcggtcct ggccgcgaag atgctgtcaa cgattgacgt gctgtccgag
     5221 gggcgtctcg tcgtgggcgt tggcgcaggt tggctcaagg ccgaattcga tgccgtcgtg
     5281 acgacgccat tcgccgaacg tgg
//
LOCUS       DATGEM010000223         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_195409, whole genome shotgun sequence.
ACCESSION   DATGEM010000223 DATGEM010000000
VERSION     DATGEM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195409"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VKQ27_06975"
     CDS             <1..203
                     /locus_tag="VKQ27_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HLY88712.1"
                     /translation="VLAIGQMLVHLIFFLHITTAPAQKTNILALLVTLLIIALMVIGS
                     LWIMSQLNVHMMPMDQLMQMQR"
     gene            complement(184..837)
                     /locus_tag="VKQ27_06980"
     CDS             complement(184..837)
                     /locus_tag="VKQ27_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88713.1"
                     /translation="MPTLQDPRHEAFAQARAKGALLIDAYESAGFVRHRGHPSRLALK
                     DNVAERIAELRASQTEAEDVSPAGLLASLRRIIKAGESSENPALVNAARLAIVDASRI
                     QAELARHQAHEREHLDWVFNDLQAREAAEAAAQAAAERPSPPREAPASAPSAPRGLPA
                     SAPRAPHNLLDSAARSPLGLPGLPLGSARLPPLRPPPLPRPAAPLARGAVSISAASA"
     gene            complement(1380..2036)
                     /locus_tag="VKQ27_06985"
     CDS             complement(1380..2036)
                     /locus_tag="VKQ27_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88714.1"
                     /translation="MGPTLEAIAKILDFVSAKRLWLFLAGVLGSGYLLFGADLGPIVI
                     STIRKNYNLPIVLIFVISAALFLSGGSTSALTALTNMLKPKHQRMTVSAIAHQSHWTV
                     SNSEDGASTTNIHIAIKLFNPGPRALQIVGSRLLYPWHFGTTPSLALTIIEEMHGNKY
                     STEAFIHTRATRNGSTAFALKGAYGGHGPSTWVRVALYDQIGGVHRFTAGLTRPDKRA
                     "
BASE COUNT          389 a          632 c          721 g          427 t
ORIGIN      
        1 tcgtgttggc tattggccag atgctcgtgc acctgatctt ctttctccac atcacgacgg
       61 ccccggcgca gaagaccaac atcctcgctt tgctcgtcac cctgttgatc atcgcgctga
      121 tggtgatcgg atcgctctgg atcatgtcgc agctcaatgt tcacatgatg ccgatggacc
      181 agctcatgca gatgcagcgc tgattgagac ggcgccacgc gcgagcgggg cggcaggccg
      241 aggaagtggt ggcggccgca acggcggcag cctggccgac ccgaggggga ggccggggag
      301 ccctaggggc gaccgcgcgg cgctgtcgag gaggttgtgg ggcgcgcgcg gggcgctggc
      361 ggggagtccg cggggcgctg acggggcgct ggcgggcgcc tcccggggcg gcgaagggcg
      421 ctccgccgcc gcttgcgcgg ccgcctcggc ggcctctctc gcctgtaagt cattgaaaac
      481 ccagtcgaga tgctcccgct catgggcctg gtggcgcgcc agctccgcct gaatgcggga
      541 ggcgtcgacg atggcgagcc gcgcggcgtt gaccagggcg gggttctcgg agctctcccc
      601 ggccttgatg atccgccgca gcgaggcgag caggcccgcc gggctgacat cctcggcctc
      661 ggtttgcgaa gcgcgaagct cggcgatccg ctcggccacg ttatccttca aggcgaggcg
      721 gctgggatgg ccccgatggc gcacgaaccc ggcggactcg taggcgtcga tcagcaacgc
      781 acccttggcg cgggcctggg cgaaggcttc gtggcgcggg tcttggaggg tcggcatggg
      841 gcaggcttgg gctcaatgag gttgatagtt gcattatgag acatctacgt agtgttgtca
      901 atttgcaatg gtcagcctgc ggcacagacg aggagcaatt ccgagaaagg gccggaggtg
      961 gctcagagga gcccttgcaa tgtccggatt cggcgtggac gctttgacgc cggccaacag
     1021 cgagttttgg gatgggatct gcgctcccgg cagttcgcgg cctgtctgag actctggatc
     1081 cgccgctcgt cggcgccagg gggttggtgc tgaggatcgc gccgctcggc tcaggggttg
     1141 gccgacggcg catagagtcg cgtgccgtcg ccgagaacgt gccggacatc cacctgccag
     1201 caaacgcgac agccgaccag ctgaagctgt tcttggcgcg atatggacgg cggggccgcc
     1261 agcgagaagg tcgcgacgac gttccactcg gctgcatgta ccgcagccca agcggcttgg
     1321 acttgcgcgc gccgccgacg ctgcgtcgac ggccgccctt tggtttggtc cacgcccacc
     1381 taggccctct tgtctggacg ggtcagcccg gcggtgaaac ggtgaacgcc gcctatctga
     1441 tcgtagaggg ccacccttac ccatgtggag ggaccatggc cgccataggc gcctttcagc
     1501 gcgaaagctg tcgaaccgtt ccgtgtcgcc cttgtgtgta taaaagcttc tgtcgagtat
     1561 ttattgccgt gcatttcctc gattatggtc aaagcaaggg atggcgttgt gccaaaatgc
     1621 caagggtaaa gcaaacgact tccaacgatc tgaagagctc tcggaccagg attaaataat
     1681 ttgattgcaa tgtgaatatt ggtcgtggaa gcaccgtctt cgctgttgct cacagtccag
     1741 tgactttggt gagcgattgc tgagacagtc attcgctgat gtttgggttt cagcatgtta
     1801 gtcagagcgg ttagcgcgct cgttgatccg ccgctaagaa ataacgccgc acttattaca
     1861 aatattaaga cgattggaag attataattc ttgcggatag tggaaatgac gatgggacca
     1921 aggtcagcgc cgaagagtag atagccggaa ccaaggacgc cggcaaggaa tagccagaga
     1981 cgcttcgccg acacgaaatc aagtatcttt gcaatcgctt cgagtgtggg tcccacgcta
     2041 tccccggccc cctacgctcc cggcgcttgg tcggaaagtc gtgaccagtc catcgctcgg
     2101 ccttagtttt cgaatctcgt tcgccgtcat acgtccccat tctgcccctg cgacccagtc
     2161 ggagtcgat
//
LOCUS       DATGEM010000224         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1443017, whole genome shotgun sequence.
ACCESSION   DATGEM010000224 DATGEM010000000
VERSION     DATGEM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443017"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /gene="shc"
                     /locus_tag="VKQ27_06990"
     CDS             <1..1287
                     /gene="shc"
                     /locus_tag="VKQ27_06990"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HLY88715.1"
                     /translation="STLDRILRVIEPLFPKWLRARAINAAVAFTEERLNGEDGMGAIF
                     PPMANAVMMYEALGKGPDYPPRATQRRGLDLLLVIGEDEAYCQPCVSPIWDTALTCHA
                     LLEAGSDEAVAPAKQGLDWLVPKQVLDVKGDWAVKAPQTRPGGWAFQYNNAHYPDLDD
                     TAVVVMAMDRARRSAPNPAYDAAIARGREWIEGMQSKDGGWAAFDVNNLEYYLNNIPF
                     SDHGALLDPPTEDVTARCISMLAQLGETAGNPSVAAGIAYLRRTQLPEGSWYGRWGLN
                     YVYGTWSVLCALNAVGVAHSDPMIRKASDWLVSIQNADGGWGEDATSYRLDYKKFDTG
                     FDGGPSTASQTAWALLGLMAAGEVENPAVARGIEYLMTTQTQKGLWDEQRYTATGFPR
                     VFYLRYHGYSKFFPLWALARYRNLRKTNSRVVGVGM"
     gene            1323..2015
                     /locus_tag="VKQ27_06995"
     CDS             1323..2015
                     /locus_tag="VKQ27_06995"
                     /inference="COORDINATES: protein motif:HMM:NF005476.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HLY88716.1"
                     /translation="MDPRPVLIVTGLIQEARIASGPGMTVICSSSDPKQLRALLTVFD
                     PTTIKGVISFGVAGGLDPSLKSGDVVVATEVLAGDARWLAGLSLNDDLIASVGLGRRR
                     IVRGRLAGAEQVVAARHLKEALHQETGAAAVDMESHIAAEYAAKAGLPFAALRVVSDP
                     AHRALPPVAMAAIKPNGDLDLRQIMRSVARNPRTLRDLVTTGIDFNRALRSLRGCRSF
                     LLGSDGLITADL"
     gene            complement(1956..>3034)
                     /gene="hpnH"
                     /locus_tag="VKQ27_07000"
     CDS             complement(1956..>3034)
                     /gene="hpnH"
                     /locus_tag="VKQ27_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HLY88717.1"
                     /translation="RYPLVLMLEPLFRCNLACVGCGKIDYPDAILNRRMTAQECWDAA
                     DECGAPMVAIPGGEPLIHKDIGEIVRGLVARKKFVSLCTNALLLEKKLHLFEPSPYLF
                     FSVHLDGLKDHHDKAVSQEGVFDRAVSAIKAAKAKGFTVNVNATIFDGHSAEDIAKFL
                     DFTVELGVGVSMSPGYAYERAPDQEHFLNRTKTKNLFRDVFARGKGKKWNFMHSSLFL
                     DFLAGNETYECTPWGMPARNIFGWQKPCYLLGEGYAKTFKELMDTTDWESYGTGKYEK
                     CADCMAHCGYEPTAATDAFKNPLKALKVAISGVRTSGPMAPEIDLTKQRPAQYIFSAE
                     VQKRLSEIRRDEAVAAQQKASTAA"
BASE COUNT          537 a          929 c         1006 g          562 t
ORIGIN      
        1 agtacgctgg accgcatcct gcgcgtgatc gagccgctgt tcccgaagtg gctgcgcgcg
       61 cgcgcgatca acgcggccgt ggcctttacc gaggagcggc tgaacggtga ggacgggatg
      121 ggcgccattt ttccgccaat ggccaacgcc gtcatgatgt acgaggcgct cggcaagggg
      181 ccggactatc cgccgcgcgc gacccagcgg cgcgggctcg acctgctcct ggtgatcggc
      241 gaggacgaag cctattgcca gccctgtgtg tcgccgatct gggacaccgc gctgacctgt
      301 cacgccttgc tcgaagccgg aagcgacgag gcggtggcgc cggccaagca ggggttggat
      361 tggctggtgc ccaaacaggt tctggacgtg aaaggggact gggcggtcaa ggctccgcag
      421 acccgccccg gtggctgggc gttccagtac aacaacgccc actatccgga cctcgacgac
      481 accgcggtcg tcgtcatggc catggaccgc gcccgtcgca gcgcgccgaa ccccgcctat
      541 gacgccgcga tcgcccgtgg tcgcgaatgg atcgagggca tgcagagcaa ggacggaggc
      601 tgggccgcct tcgacgtcaa caacctcgaa tattacctga acaacatccc gttctcggac
      661 cacggggcgc tgctggaccc cccgaccgag gacgtcaccg cccgctgtat ttccatgctg
      721 gcccagctcg gggagaccgc cgggaacccg tcggttgcgg ccgggatcgc ctacctgcgc
      781 aggacccagc tcccggaagg ctcctggtac ggccgctggg ggctgaatta tgtctacggg
      841 acctggtcgg tgctatgcgc gctcaacgcc gtcggcgtcg cgcacagcga tccgatgata
      901 cgcaaagcgt cggactggct ggtctcgatc cagaacgcgg atggcggctg gggggaggat
      961 gcgaccagct accgactcga ctataagaaa ttcgacacgg gatttgacgg cggcccgtcg
     1021 acagcctcgc aaacggcgtg ggccttgctt ggtctcatgg cggcgggtga agtggaaaat
     1081 ccagctgttg cccgcgggat cgagtacctc atgaccacac agacccaaaa gggcttgtgg
     1141 gatgagcagc gctataccgc tacgggcttt cctcgggtgt tttatctgcg ctatcatggc
     1201 tattcgaaat tctttccgct ctgggcgttg gcgcggtacc ggaatttgcg aaagaccaac
     1261 agcagggtgg taggggtcgg gatgtgaagt tgggggcagg ggttggagcg ccgtcgggca
     1321 aggtggaccc gcggccggtg ctcatcgtga ctggtctgat tcaggaggcc cggatcgcat
     1381 ccggtcccgg aatgaccgtt atctgtagca gtagcgatcc gaagcaattg cgcgcgctgc
     1441 tcaccgtatt cgatccgacg acgatcaagg gcgtcatcag cttcggcgtg gccggagggc
     1501 tcgacccgtc gctcaaatcg ggcgacgtcg tcgtggcgac cgaagtgctg gctggcgatg
     1561 cccgctggct ggcgggtctt tccttgaacg acgatctgat cgcaagcgtg gggttaggtc
     1621 gccgccgcat cgtcagggga cgcctggcgg gtgctgagca ggtggttgcg gcccgtcacc
     1681 tcaaggaggc gctgcatcag gaaaccggcg ctgctgcggt cgacatggaa agtcacattg
     1741 cggcggagta tgccgccaag gcagggctgc catttgctgc gctacgggtt gtcagcgatc
     1801 ccgcccaccg ggctttgccg ccagtggcaa tggccgccat caagccgaac ggcgatctcg
     1861 acctgcgtca gatcatgcgc agcgttgcgc gtaatccgcg aacgctgcgc gatctcgtga
     1921 cgaccgggat cgatttcaac cgcgcgcttc gcagcctacg cggctgtcga agctttctgc
     1981 tgggcagcga cggcctcatc acggcggatc tctgacagcc gcttctgcac ttccgcagag
     2041 aagatatact gcgccgggcg ctgcttggtc aggtcgattt ccggcgccat cgggcccgag
     2101 gtacggacgc cgctgatcgc taccttgagc gccttcagcg gattcttgaa cgcatcggtg
     2161 gctgcggtcg gctcatagcc gcaatgggcc atgcagtcgg cgcacttctc gtacttgccg
     2221 gtgccgtagc tttcccagtc cgtggtgtcc atcagctcct tgaaggtctt ggcatagcct
     2281 tcgccgagca ggtagcaggg cttctgccag ccgaagatgt tgcgggcggg catgccccac
     2341 ggggtgcatt cataggtctc gttgccggcc aggaagtcca ggaacaggct ggagtgcatg
     2401 aagttccact tcttgccctt gccgcgcgca aagacgtcgc ggaacaggtt cttggtcttc
     2461 gtgcggttga ggaagtgctc ctggtccggc gcacgctcat aggcgtagcc cggtgacatc
     2521 gagacgccga caccaagctc gaccgtgaag tcgaggaact tggcgatgtc ttccgccgaa
     2581 tgaccatcaa agatcgtggc gttgacgttg accgtaaagc ccttggcctt ggcggccttg
     2641 atcgccgaga cggcgcgatc gaacacgcct tcctgggaca cggccttgtc gtgatggtct
     2701 ttcaggccat ccagatgcac cgagaagaac aggtagggcg agggctcgaa caaatgaagc
     2761 ttcttctcca gcagcaacgc attggtgcag agggagacga atttcttgcg cgccacgagg
     2821 ccgcgcacga tctcgccgat gtccttgtga atcagcggtt caccgccggg gatcgcgacc
     2881 atgggcgcgc cgcattcatc ggccgcatcc cagcactcct gcgcggtcat tcgccggttg
     2941 aggatcgcat cgggatagtc gatctttccg cagcccacgc aggccagatt gcagcggaac
     3001 aacggctcca gcatcagcac cagcgggtac cgtt
//
LOCUS       DATGEM010000225         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1589328, whole genome shotgun sequence.
ACCESSION   DATGEM010000225 DATGEM010000000
VERSION     DATGEM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589328"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1877
                     /locus_tag="VKQ27_07005"
     CDS             192..1877
                     /locus_tag="VKQ27_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HLY88718.1"
                     /translation="MRRAELNRLLAVEWDYTLRTQPELATQVGDNRYNDRLSDYSEKA
                     IAADLEHSRRSLRKFEAIDVTGFPERERLNQALMVRSLRMAAEGARFKDWEMPATQFD
                     GVHLRYASLVYDTPFRNAKDYRDYLARLHQIPRALEQSADNMRAGLRDHLMPPKYILE
                     LVSAQVRQIADTPLDRNPFTDPLRKFPEAVAEADRQPLRDGIEKAVKTEVGPAYAKFA
                     KFVRDDYAPHGRLDPGIWALPDGEARYAFAVRQQTTTDLTAERIHELGLESVAEIEGE
                     MLKIARAQGFSDLKAFNTHLMQDPSLHAKSGQQLLDLYSQYRDQMYEQLPRLFGRLPK
                     NKLAVVPMESFRSADSVPADYSLGAGDGSRPGRINVNEYAPEKRLLLNVEAIAYHEGV
                     PGHHLQFSIAQELPDLPPFRRYNLDLNAYTEGWAFYAERLGKEVGFYREPYSDYGRLQ
                     NEMWRAVRWVVDTGVHARHWSRQQMIDFFHEHTAMDDQNIETEVSRYIAWPAQALSYK
                     MGQRMILQLRERAQRELGAKFDIRAFHDAVLDEGPLPLDILETKIGAWIAERK"
     gene            complement(1905..2165)
                     /locus_tag="VKQ27_07010"
                     /pseudo
     CDS             complement(1905..2165)
                     /locus_tag="VKQ27_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            2326..>2828
                     /locus_tag="VKQ27_07015"
     CDS             2326..>2828
                     /locus_tag="VKQ27_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HLY88719.1"
                     /translation="MSTIAAQTALLIAHPSTPGPAVRSLGVELRAEDPGLFVFKFLLD
                     ADISRIRVPRSGTGGRVDGLWKHTCFEAFVASADAPGYHEFNFSPSLDWAIYRFSAYR
                     AGMSPAEIGGTPEISVRRRDDGLELRSAVRVAHPADLRDARHMRIALAAVIEGEDGRL
                     SYWGLRHP"
BASE COUNT          526 a          867 c          934 g          501 t
ORIGIN      
        1 atagccgatc ctgatcacct gatcataatt ggatgagcaa gtctatggat acatcggccg
       61 ggcggagccc gctgttgccg gtcccgcggc ggcattcttc gcaccgtcgc ctgcggcgaa
      121 cctctctggc gatcggatcg ctggtcctgc tcgttccttt gtgggcgcac gcggccgttg
      181 aaaggctgga aatgcggcgc gccgaactca accgactgct ggccgtcgaa tgggactata
      241 cgctgcgcac tcagcccgag ctggcgaccc aggtcggcga caatcgctac aacgaccggc
      301 tgagcgacta ctccgagaag gcgatcgccg cggatctcga gcactcccgt cggtcgctgc
      361 gcaagttcga agccatcgac gttaccggct tccccgagcg cgagaggctc aaccaggcac
      421 tgatggtgcg cagcctgcgc atggcggctg aaggcgcccg gttcaaggat tgggaaatgc
      481 ccgccaccca gtttgacggc gtccatcttc gctacgcttc gctcgtatac gatacgccgt
      541 tccgcaatgc gaaggattat cgggactacc tggcgcggct gcatcagatc ccgcgcgcgc
      601 tcgagcagtc ggccgacaat atgcgcgccg ggctgcgcga ccatctgatg ccgccgaaat
      661 acatactgga actggtgtct gcgcaggtcc ggcaaatcgc cgacaccccg ctcgacagga
      721 atccgttcac ggatccgctg cgcaaatttc cggaggcggt tgccgaggcg gatcgccaac
      781 ctctacgtga cgggatcgaa aaggccgtca agaccgaggt cgggccggca tacgcgaaat
      841 tcgcgaaatt cgtgagggac gactacgcgc cgcacggacg tctcgatccc ggcatctggg
      901 cgcttcccga cggcgaggcg cgctatgcct tcgcggtccg gcaacagacg acgaccgacc
      961 tcacggcgga gcggattcat gagctcggac tcgagagcgt ggccgagatc gaaggggaga
     1021 tgttgaaaat cgcccgcgcg cagggcttct cggatttgaa ggccttcaac acacacctca
     1081 tgcaggatcc gagcctgcat gcgaagtcgg ggcagcaact cctggacctt tactcccagt
     1141 accgggacca gatgtacgag cagttgccgc gattgttcgg ccgtcttccc aaaaacaagc
     1201 tggccgtcgt gccgatggag tcgttccgca gcgccgacag cgttcctgcc gactactccc
     1261 tgggtgccgg cgatggttcg cgaccggggc ggatcaacgt caacgagtac gcgccggaga
     1321 agcggctgtt gctgaacgtc gaagcaatcg cttaccacga gggcgtcccg ggccaccacc
     1381 tgcaattttc gatcgcgcag gagctgcccg atctgccgcc gttccgccgc tacaatctcg
     1441 acctgaatgc ttacacggaa ggttgggcgt tctacgccga gcggctggga aaggaagtgg
     1501 ggttctaccg ggaaccttat agcgactacg gccgcctgca gaatgagatg tggcgcgcgg
     1561 ttcgatgggt ggtggatacg ggggtccatg cgcggcattg gtcgcggcag cagatgatcg
     1621 atttcttcca cgaacacacc gccatggacg atcagaacat cgaaaccgag gtgagcaggt
     1681 acatcgcctg gcctgcacag gccctctcat acaagatggg acagagaatg attctgcagt
     1741 tgcgcgaacg cgcgcagcgg gagctgggag cgaagttcga catccgtgcc tttcacgacg
     1801 cggtgctgga cgaggggccg cttccgctgg atatcctgga gacgaagatc ggtgcatgga
     1861 tcgcggagcg aaagtgagct cgccgcggcg tgcccgcgtg agttctacac accttcgtcg
     1921 cggattttag ggcgattgcg cttgcgcaga gtggagcgaa gctgaatgcc gtcgtggtcg
     1981 gcgaataatt ccgccagcca gtcgacgaaa acgcggatct tgctcgacag gtggcgattc
     2041 tcggggtaaa cgacgttgag cggtaccggc tcgggcaccc aatcgccgag caccagttcc
     2101 aaggtgccgc gctccatcgc ctctgtgagc acgaaggcgg gaatctgagc gatccccaag
     2161 cccgcctcgg cggcgcgacg cggctaagcg aaggcgcgag cgatgagctg ggtggggtag
     2221 agaaggggag ggaccgacga ggtgaggtcg cgacgggacg gcggacgggc ctggcgcttt
     2281 cgcgaaacgt gtgaccggcg ggtgtcgcga gaatacaatg gctgcatgtc gaccatcgcc
     2341 gcgcaaacgg ccctgctgat tgcccaccct tcgacccccg gccccgccgt gcgcagcctc
     2401 ggcgtagagc tgcgagcgga agaccccggc ctcttcgtgt tcaagttttt gttggacgcg
     2461 gacatctccc gcatacgcgt tccccggtcc ggcacgggtg gacgggtcga cggcctatgg
     2521 aagcacacgt gcttcgaggc tttcgtggcc tctgcggatg cgcccggata ccacgagttc
     2581 aatttctcgc cctcgctcga ctgggcgatt taccggttca gcgcctaccg ggcgggcatg
     2641 tcgcccgccg agatcggcgg gactccggag atttccgtgc gccggcgcga tgatggtctc
     2701 gaactccggt cggcggtgcg ggtggcacat ccggctgact tgcgtgacgc tcgtcacatg
     2761 cggatcgctc tggcggcggt catcgaaggc gaggatggta ggctttcgta ctgggggctg
     2821 cggcaccc
//
LOCUS       DATGEM010000226        14193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_809431, whole genome shotgun sequence.
ACCESSION   DATGEM010000226 DATGEM010000000
VERSION     DATGEM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809431"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VKQ27_07020"
     CDS             complement(<1..490)
                     /locus_tag="VKQ27_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HLY88720.1"
                     /translation="MIPRYSRPDMVAIWAPENRFRIWFEIEAHACDAQAELGVIPKEA
                     AKAVWERGAWEIDRIDEIERETKHDVIAFLTNLAEHVGPEARFVHQGMTSSDVLDTCL
                     AVQLKQAADILLADLDQLLAALKRRAFEHKNTLTVGRSHGIHAEPTTFGVKLAGYYAE
                     FAR"
     gene            complement(609..1088)
                     /locus_tag="VKQ27_07025"
     CDS             complement(609..1088)
                     /locus_tag="VKQ27_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88721.1"
                     /translation="MKRRWPAAPILAALLAACAHAPSAPTPVTQSFGDFAAPAGYAHY
                     LACPQDYCLTRPDGVTPTFKVPAEKMRVLARQAIDTQPKTQLISTANEGLRLVYQTHS
                     GGLFDHTETVTIEVVDADEGVSGLVIYGQSDIPGGDAADLKAQMEQWYAAIYRAANA"
     gene            1682..2146
                     /locus_tag="VKQ27_07030"
     CDS             1682..2146
                     /locus_tag="VKQ27_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HLY88722.1"
                     /translation="MNTASKTTPKLMTRLHHAYRHPGPARAAWVIVLALALAWVLIGS
                     ARAETAVEQTGAVVAAVAPPVHEARTPSILTALERTKRYPDAAYTSHKSLDGRAIVTF
                     DVDDGGHPQHIELVHRSGNGAFDRQTVESVRSAVCAECSGKSYRITYDYHLQ"
     gene            2375..5515
                     /locus_tag="VKQ27_07035"
     CDS             2375..5515
                     /locus_tag="VKQ27_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY88723.1"
                     /translation="MQDDTTFVFGRFRMLRRRRELLRDGIPVDLGSRAFDILLALVTR
                     NGRLATKDELMAEVWPATVVDEGNLTTHISTLRRKLGDGSAGERYILTVPGRGYRFVA
                     PIVLEDAEDGPADDGRSHPGGSREQGPARQQPSGAVAFLFTDIEGSTRRWASNHAAMM
                     DAVAVHDRMLAECVGAHAGTVFKTVGDAVCAAFADTVAAIAAAVQAQRALQAADWSEV
                     DGLRVRMAVHTGVVDLIGNDYFGLQMSKVARILSLAHGGQVIISGAAHDLASDGLPEE
                     FSLVDLGCHQLKDLPDEERIFQVVAPGLGSTFPPLRSISSKPNNLPAQLTALIGRTEE
                     TAKVKRALLMSRLGTITGTGGLGKTRLALHVAAEALPRFPDGVWLVELAAVVDPQTVI
                     AQFGKPFALRDQVGVEPLNALAAILAGMDALLVVDNCEHVLDVTAAIVARLLESCPKL
                     RVIATSTQRLGIPGEAEIRLGALSTPGPGTVKTVDDGLQFDAVRLFVERALAADAELD
                     VSESAMVPIVEICRQLDGIPLAIELAAARVRMLTLPELSKGLSRRFDILKSRDATRAS
                     RHQTLRNSIEWSYQLLTPGERDVFVRLGVFSGAFTMEAVLAVCASLELDELDIRESVY
                     SLVDKSFVSADRSGAESRFRLLASIQAYARELLEKGGRLEAFLAQHASFYGDLADRLD
                     RAFELRPPAKLDLLRSSLDNFRAALEWTIHQGNAPLPGANLAGALCDLWTMVPLLSEG
                     RGWCEAAVRRIAPDAPCFEAGRAYLALATCLNGLMAAEQACVAADHACAHFRALDQRL
                     MLARALRQRSWACGHAGDFAAGEAALEEALSLARREADRSLLVKCLLDGAIAHARRGE
                     FDDARALFSEAEAAFRELGDKKGELVSIIDRAEAAYLQGEVRSAASDLEAAAALAVEL
                     DDKRISVHCLCNLAKCRLALKEMAAAAEAAKAALPKAREIEWATGFAFAVENLAGVAA
                     EIGQCRESARLAAYAGAVYDRLGKKREAEDQERFEKLGDMLRSAMDEGEYASLAREGR
                     LMTEADAFAASLEIGL"
     gene            complement(5537..6103)
                     /locus_tag="VKQ27_07040"
     CDS             complement(5537..6103)
                     /locus_tag="VKQ27_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88724.1"
                     /translation="MNGYRIYSTILLLATLVLVPSCASREGPLRESSLNACPASPTEA
                     MPVDFSEGITADIPMKTTENKCIHVLVSKINVHGNVVCVVNVFDPWTNRVHPSRVRLN
                     TPSAAGQEQDLIVDCDKGLSFERVAAEPAPPAPPPPSLGAPPPLPPTPVASIGPCQRP
                     SAFLQHLIFKTANSQPLQLSPGTNCIGG"
     gene            complement(7405..7489)
                     /locus_tag="VKQ27_07045"
     tRNA            complement(7405..7489)
                     /locus_tag="VKQ27_07045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7453..7455),aa:Leu,seq:taa)
     gene            complement(7538..8311)
                     /locus_tag="VKQ27_07050"
     CDS             complement(7538..8311)
                     /locus_tag="VKQ27_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HLY88725.1"
                     /translation="MTEAPAKPVTACLVIIGNEILSGRTQDLNLNYLAKGLNEVGVQL
                     RETRVIPDVPDTIVATVNEVRAKFDYVFTTGGIGPTHDDITAECVARAFGVKLILHPE
                     AKRRLEAHYRPGEFNEARMRMAHVPEGATLIDNPVSTAPGFRIGNVHVMAGVPKIMQA
                     MFDGIRHDLKGGAKVLSRSVTCALAEGTLAKDLGAIQADYPTVEIGSYPFWTRSGFGV
                     SLVLRSADAPALEHASEAVIAMIRGFGGEPIEREPDFKE"
     gene            8404..9105
                     /gene="sfsA"
                     /locus_tag="VKQ27_07055"
     CDS             8404..9105
                     /gene="sfsA"
                     /locus_tag="VKQ27_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HLY88726.1"
                     /translation="MIFPAPLVRGRLVRRYKRFLADVALDSGEVITAHCANPGAMLGV
                     APPDAEVWLSPSRNPARKLAWSWELVRIGQGLVGINTSWPNLLAEEGIRAGAIPELAG
                     YPMLSREVRYGRNSRIDLLLEAPGRPICYVEVKNVHLSRGDGLAEFPDCVTQRGAKHL
                     LELADMVAAGYRAVMLYVVQRDDCDRFRTAADFDPAYHAGLKVALAAGVEALVYGCAV
                     TVEGIRVDRKLELLA"
     gene            9166..9993
                     /gene="map"
                     /locus_tag="VKQ27_07060"
     CDS             9166..9993
                     /gene="map"
                     /locus_tag="VKQ27_07060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLY88727.1"
                     /translation="MTDLALSDRSYAEEARIKVHAPEDFAWMRKAGRLAAEVLDYITP
                     FVKPGVTTGELDRLCHEFILDHKAIPAPLNYRGFPKATCISINHVVCHGIPGDRKLMD
                     GDIANIDITVILDGWYGDTSRTYLIGDKVPLKARRLVDVTYDAMMLGIAAVKPGAHLG
                     AIGYAIQSFAESHRFSVVRDFCGHGIGKVFHAAPSVLHYGRPNEGPVLKEGMFFTIEP
                     MINGGRYEVKVLADGWTAVTKDKSLSAQFEHTVGVTADGCEIFTLSPAGHTKPPYSL"
     gene            10001..10555
                     /gene="radC"
                     /locus_tag="VKQ27_07065"
     CDS             10001..10555
                     /gene="radC"
                     /locus_tag="VKQ27_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLY88728.1"
                     /translation="MGEAEDGQGDKPGYLGHRQRLRARFLEAGPAALPDYELLELILF
                     QAKPRQDTKPLAKALLARFGSFAGVIGADIPALMAVPEMGEASVVALKAIQAGVERVL
                     RDEVRTAPVLSSWDKVVDYCRAAMARAPIEQFRLLFLDRKNALIADEVQQTGTVDHTP
                     VYPREVVKRALELSASAIIMMLAA"
     gene            complement(10565..11617)
                     /locus_tag="VKQ27_07070"
     CDS             complement(10565..11617)
                     /locus_tag="VKQ27_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88729.1"
                     /translation="MPDDRLIAATGLIGPWPIPPSHAQVAEIFHAFMAARSTRVIPGY
                     SRTLASKRLVPAPLFRFDTNDGKTKRFYCSTCNTPNKFGAGVAVWHEDGWIRLIGCKC
                     GAEFYEHTDFAARIDEMDRLAKRRDRLASFPAILLAAREVRRDINALCASSLLSEMGR
                     FRISLEKKAQALYHELHYSVTNGNGNLVISRKLTQPKNIGGRRVTHEDFPFAAFRGHR
                     ILRHDVALRNSLDSALSILLENHILLSGGKAADVDDRRQQKAVGRYREAEITLRQAKD
                     HLENALAFFGSTNRTVIAKWATENPNINGRLRATRAGWVWSGDHDDVTFDVAELMPRP
                     ILPSLDALTRALKPQL"
     gene            complement(11802..12746)
                     /locus_tag="VKQ27_07075"
     CDS             complement(11802..12746)
                     /locus_tag="VKQ27_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88730.1"
                     /translation="MTTDEGLHFSGSAAPASQENTKLAGASPTPASGGPVSPSTPLSR
                     SSDPSFVTPMEDTSATQEAGFDLQDLDEELLLSELAAACPDLADPRVTMLFDYLDQLD
                     MPRREPRIDGYGGQEKSILWTLAGMRLAVDHRLFDLRHVTVNLHQAGPEKVESADDAI
                     KTIGRNLKPALGDMPFLWWGIHVPARISGEPQQAHLHLVVALPLDDGKAAEALDRCRV
                     RRNRNKERSVHEKEVFDPAGLLEYWSLKRNLGVKGARPIACQPIKSRCGEMIETHIQA
                     EISDLRDDLADAVMMGDSGRPGEREHSFSPVARALLAK"
     gene            complement(12922..13581)
                     /locus_tag="VKQ27_07080"
     CDS             complement(12922..13581)
                     /locus_tag="VKQ27_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88731.1"
                     /translation="MRILSLCRVILLMASVSACVSHSAPTWYAADQYDKFKNESSCRA
                     SIGSESVGAFTYTFNGQYYPFIERTGDNVYVGISSVPLAGANVTTFRPSAPIPVGDVE
                     LKVDGNETWVLAAAESPVHTVAGSSSELMRQQTQQQIAAMAPANPQLAGSLAAMQDSM
                     RGMTAPYTAASGDKARQIIDQMRAGRVVAFRATMAMGGISKSFEYPLTGLREQLARCG
                     I"
     gene            13937..>14193
                     /locus_tag="VKQ27_07085"
     CDS             13937..>14193
                     /locus_tag="VKQ27_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88732.1"
                     /translation="MSEALEGTTNETDGGGGLSVVGDALGDLLTGIPAPIRKNAAKAF
                     ARLCTAAVEYPVTLIEGAIAEKRAESQARVKLIDASASQIA"
BASE COUNT         2529 a         4376 c         4586 g         2702 t
ORIGIN      
        1 tgcgggcgaa ttcggcgtaa tagccggcga gcttgacgcc gaaagtcgtg ggctcggcat
       61 ggatgccgtg gctgcgcccg acggtcagcg tgttcttatg ctcgaatgcg cgccgcttga
      121 gcgccgccag cagctggtcg agatcggcca gtaggatgtc ggccgcctgc ttcagctgga
      181 ccgcgaggca ggtgtcgagc acgtcggacg aggtcatgcc ctggtggacg aagcgcgcct
      241 ccgggccgac atgctcggcg aggttggtca ggaacgcgat gacgtcatgc ttggtttcgc
      301 gctcgatctc gtcgatccgg tcgatctccc aggcgccgcg ctcccagacc gccttggcgg
      361 cttccttcgg gatcacgccc agctcggcct gcgcgtcgca ggcatgggcc tcgatctcga
      421 accagatacg gaagcggttt tcgggcgccc agatggcgac catgtcgggg cggctgtaac
      481 gcgggatcat tgggggcgct cttgtcgaaa gggaccggtt gcgagccctc ttcaatagca
      541 gccgcgtcgc ccgctgccca acgcaaagcg cgaaggggac gatcccgccg gctgcggggc
      601 agccgggctc aggcgttcgc cgcccgatag atcgcggcgt accattgctc catctgcgcc
      661 ttgaggtcgg ccgcgtcgcc gcccggaatg tcggactggc cgtagatcac caggccgctc
      721 acgccctcgt cggcgtcgac gacctcgatc gtcacggttt cggtatggtc gaacaagccg
      781 ccggaatgag tctggtagac caggcgcagg ccctcgttgg cggtcgagat cagttgcgtc
      841 ttcggctgcg tgtcgatggc ctgccgggcg aggacgcgca tcttttcggc cggcaccttg
      901 aaggtcggtg tgacgccgtc gggccgggtc aggcaataat cctgcgggca ggcgaggtaa
      961 tgggcgtagc cggccggggc ggcgaagtcg ccgaaactct gggtgaccgg cgtcggcgcg
     1021 ctcggcgcgt gggcacaggc cgccagcagc gcggcgagga tgggggccgc gggccagcgc
     1081 cgcttcacag ggcttcgccc gcgttccggt ctcgtcggca tgaactcgcc cctcgctcga
     1141 tggcctcgcg ggccggacag taatcatccg ccgcccgccc ggacaagggg cgtctgacgg
     1201 cccccccgat cggagcattt ggcgaaaatc gacaaaaaaa gcccctggat cgctttttgc
     1261 ggcggttcag gggttgctta ggcgataagg cgccctatct gtgcagtgca gcatgagccc
     1321 tatcggttct gctgcacgcc ttttgggcgt ttcctcccta gactcaggcc gtgcgaaagt
     1381 gcggcctttc tttttggcct catcgttgac cgcaaggccg gttgccgagc caacacgcgt
     1441 cagcgggatt ggttcccaga tcttttgatg ttgggcgagt cgccgcaaac ctgaagttgc
     1501 ggccttcgga atgcggtcat cggccacgcc cgaggcatgc ctttcggctc gagccgggct
     1561 tttccgcctt tggaactctt gagaacgttt gagatccgtc tcggcgaccc ttgaggtgcg
     1621 caggcgcaat tctctgtccg tcagccgctc atggccgaca tgaatgacag ggaaagccat
     1681 catgaatacg gcatcgaaaa ccactccgaa gctgatgacg cgtctccacc acgcctatcg
     1741 gcacccgggg ccggcccgcg ccgcttgggt gatcgtcctg gcgctggcgc tggcctgggt
     1801 gctgatcgga tccgcaaggg cggaaacggc ggtcgaacag acgggagccg tcgttgcggc
     1861 ggtggcgccg ccggtgcacg aggcgcgcac gccgtcgatt cttacggcgc tggaacggac
     1921 caagcgatac ccggacgcgg cctacacgag ccacaagtcc ctggatggac gggcgatcgt
     1981 gacgttcgac gtcgacgacg gcggccaccc gcagcacatc gagctcgttc atcggtccgg
     2041 gaacggcgca ttcgaccgcc agaccgtgga atccgtccgt tccgccgtct gcgccgaatg
     2101 cagcggaaaa tcctatcgga tcacgtacga ctaccatctc cagtagaagc cggcgccacg
     2161 accaatcacg tcacaagaac ctccggagat tgaaagtcgc cagctcgaag cctcgggctg
     2221 gcgacttcgg cttgttccgg catctggcaa tccttcccaa aatgccgaac ccctcgatcg
     2281 ttgtcggcgt taatcgcagg cattacactc gcgtgcgacg catgttggct cctacgttcg
     2341 ccgagaaaac ggcagacatc gcgaggcccg gccagtgcag gacgatacga ccttcgtttt
     2401 cggccgattt cgcatgcttc gccgccgccg cgagttgctt cgtgacggca tcccggtcga
     2461 cctgggcagt cgcgccttcg acattctcct ggcactcgtc acccgcaacg gccgtctcgc
     2521 gaccaaagat gagttgatgg ccgaagtctg gccggcaacc gtcgtcgacg aaggcaatct
     2581 gaccacccac atctcgaccc tgcgccgcaa gctcggagat ggcagtgccg gggagcggta
     2641 tattctcacc gtgccgggcc ggggatatcg cttcgtcgcc cccatcgtgc tcgaagatgc
     2701 cgaggatggt ccggccgacg atgggcgatc gcatccgggc ggatcgcgcg agcagggccc
     2761 tgcgcgccag cagcccagcg gcgcggtcgc cttcctcttc accgacatcg aaggcagcac
     2821 gcggcggtgg gccagcaacc acgcggccat gatggacgcc gtcgccgtcc atgatcgaat
     2881 gctcgccgaa tgcgtcggcg ctcatgccgg aacggtgttc aaaacggtcg gggatgcggt
     2941 ctgcgcggcg tttgcggaca ccgtcgccgc gatagcggct gcggtacagg cacaaagggc
     3001 gctccaggcg gcggactgga gcgaggtcga cggtctgcgc gtcaggatgg cggtccatac
     3061 cggcgtcgtc gacctgatcg gcaacgacta tttcgggctg cagatgtcca aggtcgccag
     3121 aatcttgtcc ttggcccatg gcgggcaggt catcatctcg ggcgccgccc acgacctagc
     3181 gagcgatggc ttgccggaag agttttcgct ggtcgacctc ggctgccatc agctcaagga
     3241 cctcccggac gaagagcgga tttttcaggt cgtcgccccc ggcctcggct ccactttccc
     3301 gccgctgaga tcgatctcgt ccaagccgaa caacctgccg gcccagctga cggcgctgat
     3361 cggacggacc gaggagacgg ccaaggtcaa gcgggcgctg ctgatgtccc gtctcggcac
     3421 gatcacgggt accggcgggc tcggcaagac ccgccttgcg ctccatgtcg cggcggaagc
     3481 gctcccgcga tttcccgatg gcgtgtggct ggtcgagctc gccgcggtcg tcgatccgca
     3541 gacggtgatt gcgcaattcg gcaagccgtt tgccctgcga gaccaggtcg gcgtcgagcc
     3601 gctcaacgcc ctggcggcga tcctcgcggg catggatgca ttgctcgtcg tcgataattg
     3661 cgaacatgtt ctggacgtga cggccgcgat tgtcgcgcgc ctgctcgaga gctgtccgaa
     3721 gctccgcgtc atcgcgacga gcacgcagcg gctcgggatc ccgggcgagg ccgaaatcag
     3781 gctgggggca ctcagcacgc cggggccggg aacggtcaag acggtcgacg atggcctgca
     3841 attcgacgcg gtgcgcctgt tcgtcgaacg ggcgctggcg gccgacgcgg aactggacgt
     3901 ttccgaaagc gcgatggtgc cgatcgtcga gatctgccgc cagctcgacg gcattccctt
     3961 ggccatcgaa ctggccgcag cccgggtgcg catgctgacg ctgccagagc tttccaaggg
     4021 gctgagcaga agattcgaca tcctgaagag ccgcgatgcc acccgtgcct cccgccacca
     4081 gacgctgcgg aattccatcg aatggagcta tcagctgctc actcccggcg aacgggacgt
     4141 cttcgtgcgg ctcggcgtct tctccggtgc cttcaccatg gaggccgtgc tggcggtgtg
     4201 cgcctccctc gagcttgacg agctggacat acgggaaagc gtctattccc tcgtcgacaa
     4261 gagcttcgtg tccgcggatc gtagcggagc cgaaagtcgg ttccgtttgc tcgcgtccat
     4321 tcaggcctat gcccgtgaac tgcttgagaa gggcgggcgg ctggaggctt tcctggcgca
     4381 gcatgcgtcg ttctatggcg atctcgccga ccggctggat cgggctttcg agctgcgtcc
     4441 gcccgcaaag ctcgacctgc tgcgcagctc tctcgataat ttccgggcgg ccctggagtg
     4501 gaccatccac caggggaacg cccccctgcc gggcgccaat ctcgccggcg ccttatgcga
     4561 tctctggacg atggtgcccc tgctgtcgga ggggcgcggc tggtgcgagg cggcggtccg
     4621 gcggatcgcg cccgatgccc cgtgcttcga ggcgggcagg gcctatctgg cgctcgcgac
     4681 ctgcttgaac ggcttgatgg ccgccgaaca ggcttgtgtt gccgccgatc atgcctgtgc
     4741 gcatttccgg gcgctggatc agcgtctcat gctcgcgcgc gccctcaggc agcgctcctg
     4801 ggcctgtggc cacgccggcg acttcgccgc gggcgaggcg gcattggaag aggcgctatc
     4861 gctggcgagg cgcgaagccg acaggtcgtt gctggtcaaa tgccttcttg acggtgcaat
     4921 cgcccacgcc agacggggcg agttcgacga tgccagggcg ctgttctccg aggcggaagc
     4981 cgcgttccgg gagctgggcg acaagaaggg cgagctggtc agcatcatcg atcgggcgga
     5041 agcggcatat ctccagggcg aggtccggtc ggcggcgagc gatctcgaag ccgccgccgc
     5101 cctggccgta gaactggatg acaagcgcat ctccgttcac tgcctctgca acctcgccaa
     5161 gtgccggctg gcgctgaagg aaatggcggc tgccgccgag gcggccaagg cggccttacc
     5221 caaggcgcgg gaaatcgaat gggcgaccgg ctttgccttc gccgtcgaga acctcgccgg
     5281 ggtggccgcc gaaatcggac aatgtcgcga gtctgccagg ctcgctgcgt acgcgggcgc
     5341 ggtttacgac cggctgggca agaaacggga agcagaagat caggagcgct tcgagaagct
     5401 aggcgacatg ctgcgttccg ccatggacga aggcgaatat gcctcgctgg cccgcgaagg
     5461 acggctcatg acggaagccg acgcctttgc cgcaagcctg gaaatcggct tgtgacgcat
     5521 tgcgtcggct tcgccatcaa cccccgatgc agttcgttcc cggtgacagt tgcaggggct
     5581 ggctgttcgc ggtcttgaat atcaagtgct gcaggaacgc actcggccgc tggcaaggcc
     5641 cgatcgacgc gaccggcgtc ggcggcagag gcggtggggc ccctagggac ggcggcggcg
     5701 gtgccggcgg cgcgggctca gccgccacgc gttcaaagct cagtccctta tcgcaatcca
     5761 cgatcaggtc ctgctcctgg ccggccgcag agggggtatt cagccggacg cggcttggat
     5821 ggacgcgatt ggtccatgga tcaaagacgt tcaccacgca tacgacgttt ccatgcacat
     5881 ttatcttgga gacgagaacg tggatgcact tgttctcggt cgttttcatc gggatgtcag
     5941 cggtgatccc ttccgagaaa tcgaccggca tggcttcggt gggcgaggcc gggcacgcat
     6001 tcaaggaact ctctcgcaga gggccttctc tggaggcaca actcggcacc aagaccaggg
     6061 tggcaagcaa caggatggtc gaataaatcc gatatccgtt catcgcatca cctcaaaagg
     6121 agaaggctgc cttccaaatg gcgccggcct tggtgagcag agtactgcaa ccgcattcga
     6181 gacgggaccc gttcgatggc gccgggccat gttcccttga gcggacgcat tcgccgaaag
     6241 tccgaagatc ggcggcttat ttcatcgtta attcatgtga tactcgcaat tgggtagggg
     6301 ggatagttca taagtggttt ggctcgacgg cgtagtggtc ttaagtatca aatgttgtag
     6361 aaatgcattt gatgtattgc aggatattag tgagatctgt ccttgtgcgg ccggcggcgt
     6421 agcggctgtg ccagtggtgg agcgtcccat ataagtaacg ttatctattc tgcaatcatc
     6481 gatgaaatct tgattgctat tgccgtgggg acctgggtcc aaacgcacac ggtgcgaatg
     6541 tttgtatttt cttgatggat cgtaggcgct gactaggcat atatcgtgcc cgtttatatt
     6601 tattgtggtg attgagacat acccgcaggg attatgggca tcgtttgttg ccatgggaat
     6661 ttgttgggta tttccttcgt cgaaagcgag ctgtgtaagt gccgacggaa gagcggggca
     6721 tgccgctgga gggtttcctt gcagtccaaa atcctgcgtt gcgcaacctt gcacaaggtt
     6781 gagtgcggga agcagggaga tggccgaata tttccaatat ccattcatcg catcacctca
     6841 caggtaaaaa tattgccgcg acatctgccg tggtgttggc atgcgaggtc ttgcggccgg
     6901 catgcaacgg cgttctgtct tggccatttc ccagtggcct ttcttcagaa gtgctgaatt
     6961 cgggcgatcg ccaatcggcg atgggcacta ttcctcgaat atctaaggcg cggccgatgg
     7021 cgctcgcgac cagcatgggc aatgcccatt cggaggttcg aaggaacgca aacgcgatgt
     7081 tgagtagaaa ataatatacc agttcagcgg ggactcaagt ataaatctgt gcctgacgga
     7141 cgtcagtggg gtgaaaattc ttgaaggcgc caccgctcga cgcttggtgc ggttggaaat
     7201 gctttggtca ccggcgtatc gtgagcgctg ccacggcttc acggcgaaaa tcgccgacgt
     7261 ttcggggtgc ccgccgaatt cctttattcg atggttcggg caaggcttgt tgcggacgag
     7321 ctttcgcatc ggtgcagatc cggccatcgc atcggcgtag cccggcatgt gcgatgcttt
     7381 tcagggtgaa acgcttcagt cgcttggtga ggacggcggg actcgaaccc gcatgcccga
     7441 aggcgtcaga ttttaagtct gaggtgtcta ccggttccac cacgtcctcg accgggcgag
     7501 agcagtagca tcaaggctgc gtgtccggca accgatgcta ttccttgaaa tccggctcgc
     7561 gctcgatcgg ctcgccgccg aagccgcgga tcatggcgat gacggcctcg ctcgcatgct
     7621 cgagcgccgg ggcatcggcg ctgcgcagca ccagcgaaac gccgaagccg ctgcgcgtcc
     7681 agaacggata cgagccgatc tcgaccgtcg gatagtcggc ctggatcgcg cccaggtcct
     7741 tcgccaaggt gccttcggcc agcgcgcagg tgacgctgcg cgacagcacc ttggcgccgc
     7801 ccttgaggtc atggcggatg ccgtcgaaca tcgcctgcat gatcttgggc acgccggcca
     7861 tgacatggac gttgccgatg cggaagccgg gcgcggtcga gaccggattg tcgatcaggg
     7921 tcgcaccctc cggcacatgg gccatgcgca tgcgcgcttc gttgaattcg cccggccggt
     7981 aatgcgcctc gagccggcgc ttcgcctccg gatgcaggat cagcttgacg ccgaaggccc
     8041 gcgccacgca ctcggcggtg atgtcgtcat gggtcgggcc gatgccgccg gtcgtgaaga
     8101 catagtcgaa tttcgcccgc acctcgttga cggtcgcgac gatggtgtcc ggcacgtcgg
     8161 ggatgacgcg tgtctcgcgc agctgcacgc cgacctcgtt caggcccttg gccagataat
     8221 tgaggttcag gtcctgggtg cggcccgaca ggatctcgtt gccgatgatc acgaggcagg
     8281 cggtgacggg cttcgcgggg gcttcggtca tgatggaatg aaagctagga caggaacggc
     8341 cgctcggcaa ggcgcgccgg ccggcccttg cggcggtgtg ccggaaccgc taaagcggcg
     8401 gccatgatct ttcccgctcc cctggtgcgc ggccggctcg tccgccgcta caagcgcttc
     8461 cttgccgatg tggcgctcga cagcggcgag gtgatcacgg cccattgcgc caaccccggc
     8521 gcgatgctgg gcgtggcgcc gcccgatgcc gaggtttggc tgtcgccgtc gcgcaacccg
     8581 gcgcgcaagc tcgcctggtc gtgggaactg gtgcggatcg gccaagggct ggtcggcatc
     8641 aacacatcct ggcccaatct gctggcggag gaggggatcc gcgccggtgc cattccggag
     8701 ctcgccggct atcccatgct cagccgcgag gtgcgctatg ggcgcaacag tcgtatcgat
     8761 ctgttgttgg aggcgcccgg gcgcccaatc tgctatgtag aggtcaagaa cgtccatttg
     8821 agtcgcgggg acggtctggc cgaatttccg gattgcgtca cccagcgtgg ggcgaagcat
     8881 cttctggaac tggccgacat ggtcgctgcc ggctatcgcg ccgtgatgct ctatgtcgtt
     8941 cagcgcgacg attgcgaccg gttccgcacc gccgccgatt tcgacccggc ctatcatgcc
     9001 ggtctcaagg tggcgctggc ggcgggtgtc gaggcgctgg tctacggttg cgccgtgacg
     9061 gtcgagggca tccgggtcga ccggaaactg gagctgctgg cctagcgggc cccgaagcgg
     9121 catgatgaac gaacgggcac gagcccctgg agttgaagga cgacgatgac ggatctggcg
     9181 ctcagcgatc gcagctatgc cgaggaagcg cgcatcaagg tgcatgcgcc cgaggatttc
     9241 gcctggatgc gcaaggccgg caggctggcg gccgaggtgc tcgactacat tacgccgttc
     9301 gtgaagccag gggtcaccac cggcgagctc gaccggctct gccatgaatt catcctcgac
     9361 cacaaggcga tcccggcgcc gctcaattat cgcggcttcc ccaaggcgac ctgcatctcg
     9421 atcaaccacg tcgtctgcca cggcatcccg ggcgaccgca agctgatgga cggcgacatc
     9481 gccaacatcg acatcacggt catcctcgac ggctggtacg gcgacacgag ccgcacctac
     9541 ctgatcggcg acaaggtgcc gctgaaggcg cgccggctgg tcgacgtcac ctatgacgcg
     9601 atgatgctgg gcatcgccgc ggtcaagccg ggcgcgcacc tgggcgccat cggctacgcg
     9661 atccagagct tcgccgagag ccatcgcttc tcggtcgtgc gcgatttctg cggccacggc
     9721 atcggcaagg tgttccacgc ggcgccgagc gtgctgcatt acggccggcc gaatgagggc
     9781 ccggtgctga aggaaggcat gttcttcacc atcgagccta tgatcaacgg cggccgctac
     9841 gaggtgaagg tgctggccga cggctggacc gccgtcacca aggacaagtc gctgtcggcc
     9901 cagttcgagc atacggtcgg cgtcacggcc gacggctgcg agatcttcac gctgtcgccg
     9961 gccggccata ccaagccgcc ctattcgctc tgagttgccg atgggcgagg ccgaggacgg
    10021 gcagggcgac aagcccggtt atctcggcca tcgccagcgc ctgcgcgccc gcttcctcga
    10081 ggcggggccg gcggcattgc cggactacga gctgctcgaa ctgatcctgt tccaggcgaa
    10141 gccgcgccag gacaccaagc cgctcgccaa ggcgctgttg gcacgcttcg gcagcttcgc
    10201 gggcgtgatc ggcgccgata ttccggcgct gatggccgtg ccggagatgg gcgaggcatc
    10261 ggtcgtggcc ttgaaggcga tccaggccgg ggtcgagcgg gtcttgcgcg acgaggtgcg
    10321 cacggccccc gtgctgtcgt cctgggacaa ggtcgtcgac tattgccggg ccgccatggc
    10381 gcgggcgccg atcgagcagt tccgcctgct gttcctcgac cgcaagaacg cgctgatcgc
    10441 cgacgaggtg cagcagaccg gcacggtcga ccacaccccg gtctatccgc gcgaggtagt
    10501 gaagcgcgcg ctggaactga gcgccagcgc catcatcatg atgctagccg cgtagttgcg
    10561 accgctataa ttgcggcttc agcgcccgag ttagcgcgtc cagcgaagga aggatcggtc
    10621 gcggcataag ttcggcaaca tcgaatgtca cgtcgtcgtg atccccgctc cacacccacc
    10681 cagcacgagt ggcgcgaagc ctgccgttga tattcgggtt ttcggtagcc catttggcga
    10741 tcaccgttcg gttggtgctc ccgaagaatg ccagcgcatt ctctaggtgg tccttggcct
    10801 gcctaagcgt gatctctgcc tcgcggtatc gtccaactgc tttctgttga cgccgatcgt
    10861 caacgtcagc ggcctttcca ccgctcagta ggatgtggtt ctctagtagt atcgatagtg
    10921 cgctatcgag agaatttctt agcgccacat cgtgacgaag gatgcggtga ccgcggaaag
    10981 ctgcaaatgg aaaatcctca tgtgtcacgc gccttccgcc gatattttta ggctgcgtca
    11041 attttctact aataactaag tttccatttc cgtttgtgac gctatagtgg agttcatggt
    11101 agagagcttg cgctttcttc tctagagata ttcggaatcg gcccatctct gaaagcaggc
    11161 ttgaagcaca caatgcgttg atgtcccgcc ttacctctct cgcggctaag aggatagcgg
    11221 ggaaggatgc taggcgatcc cgacgcttcg caagccggtc catttcgtcg atccgagcag
    11281 caaagtcggt gtgttcatag aactcggcac cgcacttaca gccaatgagt cgaatccacc
    11341 cgtcttcgtg ccagaccgca accccggctc caaacttgtt cggcgtgttg caggtcgagc
    11401 agtagaagcg cttggttttt ccatcgttcg tgtcgaaacg gaacagcggc gccggtacca
    11461 gacgtttcga agctaatgtg cgggaatagc cggggatcac tcgcgtcgag cgggcagcca
    11521 taaacgcatg aaaaatctcg gcgacctgtg cgtgggacgg agggataggc cacggtccaa
    11581 tcaatccggt agcggcaatc aggcgatcat ccggcatcgc gtcccacgcc cttctttgaa
    11641 ccagatatgg cacgaaggcg ggacactgac acaatatatc gatgttgcca agatgttctt
    11701 gtaacctctt ggcgacaccg cgcccggctg ttctcccgat tgttagcggg cgccgaacga
    11761 tagcgagtgg gcgacccggg gctaggctgt cgtccagcgc gtcatttcgc gagaagcgcc
    11821 cgggcgaccg gcgagaagct atgctcgcgc tcgcccggcc gtccagagtc gcccatcatc
    11881 acggcatcag ccagatcgtc ccggagatcg ctgatctccg cctgaatgtg ggtctctatc
    11941 atctccccgc accggctctt gatgggctgg cacgcgatcg gccgcgcgcc cttgacgccg
    12001 agattgcgct tgaggctcca gtattcgagc aggccggccg ggtcgaagac ctctttctcg
    12061 tgcaccgacc tctccttgtt ccggttgcgg cgcacccggc agcggtccag cgcctcggcg
    12121 gctttaccgt cgtcgagcgg cagcgcgacg acaagatgca ggtgcgcctg ctgcggctct
    12181 cctgagattc tggcgggtac atggatgccc caccatagga acggcatgtc gccaagcgcg
    12241 ggtttcaggt tacggccgat cgtcttgatc gcatcgtccg cactctcgac cttttcgggg
    12301 ccagcctggt ggagattaac cgtgacgtga cggagatcga atagccggtg atcaacggca
    12361 aggcgcatcc cggcgagtgt ccagaggatg gacttctcct ggccgccata tccatcgatg
    12421 cgcggctcac gccggggcat gtcgagttga tcgaggtagt cgaacagcat cgtgacccgt
    12481 gggtccgcga gatcagggca tgcagcagca agctcgctca gcagcaactc ttcgtcgaga
    12541 tcctggagat cgaatccggc ttcttgagtt gcgctggtat cctccatcgg tgtgacaaag
    12601 gaaggatctg atgatctgga aaggggtgta gaaggactaa ctggtcctcc actggccggt
    12661 gtcggcgacg ccccagccag cttggtgttt tcctgggacg ccggggcggc gctgccggaa
    12721 aaatgtaacc cttcatcggt cgtcaacgag gtcgtattca tagcctgatc gggccccttt
    12781 ctaccgctgc atcccgatcc taccgagcgc aaaaaagccg ctgttcgcgc ggacgcgcct
    12841 ttcctggcgc ggccaacaca aacagcggct cgctaccgat atggtgacgc cgatctccgg
    12901 ttcaaggtcg gcgcgtgccg gtcagatccc gcagcgcgcc agttgctcgc gcaggcccgt
    12961 aagcgggtat tcgaaagact tgctgatgcc gcccatagcc atcgtcgccc ggaacgcgac
    13021 tacccggcct gcgcgcatct ggtcgatgat ctgacgggct ttgtcgccag aggcagcggt
    13081 atagggcgcc gtcatgccgc gcatgctgtc ttgcatggcc gctaaggagc cggccaactg
    13141 agggttagca ggtgccatcg ccgcgatctg ctgctgcgtc tgttgcctca ttagctcgct
    13201 cgaagacccg gcaaccgtgt gtaccggcga ttcagccgcc gcgaggaccc acgtctcgtt
    13261 accatcaacc ttcaactcca cgtccccgac ggggataggc gcgctcggtc ggaacgtcgt
    13321 cacattggcg ccggctagcg gcacgcttga aatgccgacg tagacgttat ccccggtcct
    13381 ctcgatgaac ggatagtatt gaccattaaa cgtataggta aacgccccta cagactccga
    13441 tccgatactc gctctgcacg acgactcatt tttgaatttg tcgtactgat cggccgcgta
    13501 ccatgtcggc gccgaatgtg aaacgcaagc agacacgctc gccatcagca atataacacg
    13561 gcacaatgat aaaatgcgca ttcaactctc caaggcgtaa tcctggatcg agtctagctg
    13621 aggacctcaa tgacgttcaa ctggtagcat gaagactaag gcgccccctt ccggcagcct
    13681 gttccctgtt gcccgatttt cggcgaccgc agtgaataag ccccgctggc gaaggggcgg
    13741 catcgcgttt tttttggtgc cggccgcggt agatacagga gcggccgacc ccttctagag
    13801 gcagtcagcg ccaggaacct agtttggccc tcggagagct ctacctaaca tccaaattcg
    13861 cgaataggat gaagccagct cggcgagcac ccgcatccgg atgcggccag tttaccccga
    13921 cacatgagat ttgctgatga gcgaggcact ggaaggaacc accaatgaaa cagacggggg
    13981 cggcgggctg tccgttgtag gcgacgctct aggcgacctc ctcacgggca ttccagcacc
    14041 gatccgcaag aacgcagcca aagcgttcgc ccgcctttgc acggctgcgg tcgaatatcc
    14101 cgtaacgctg atcgaaggcg ccatcgcgga gaagcgggca gagtcgcagg cacgcgtgaa
    14161 gttgatcgac gcttccgcaa gtcagatcgc aat
//
LOCUS       DATGEM010000227         5677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1165427, whole genome shotgun sequence.
ACCESSION   DATGEM010000227 DATGEM010000000
VERSION     DATGEM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165427"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VKQ27_07090"
     CDS             complement(<1..661)
                     /locus_tag="VKQ27_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88733.1"
                     /translation="MLNIQTFDNRAGGNVLYKALSHPLAAEAIARLYTRLEGQVALYD
                     PEGIAAALLALYPDAPAHTRYVQDVTAVGPQALPLTDLANCKARTVLIAAFDARRASA
                     RIADLLPAGAAVLTLDEARLPAELLTNSGRYLDKQNFATNFAFFRDADGLSTRLVSAN
                     YWSGYGGGAIRLWLRLFDADGGALATWQQEVPAGPCGFSIDSREVRRRFKLPAFTGQL
                     FI"
     gene            complement(655..1599)
                     /locus_tag="VKQ27_07095"
     CDS             complement(655..1599)
                     /locus_tag="VKQ27_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HLY88734.1"
                     /translation="MSAAATSWILSEGYAGLQAQALGLAEAACLAPELRVLVPRAPWK
                     WVPASLWPAPLAAVRDAVRAPLPELVIGCGGVAAAVGAALRRGGRPVVQVQHPRMDPR
                     RFDVVVVNRHDELTGPNVVVTRTALHRVTPARLAEAGSVWAPRLEHLKRPLVAVLVGG
                     SNGRFRLDAEVAGELASQLAAMMQRDGVGVAVTPSRRTDPAAVRVLAEALIPLGAYVW
                     DGAGDNPYFGMLALADAIVVTVDSVSMVSEAAATSAPVLLAKLPGRSRRNGLFIDCML
                     AEGRVREFAGRFEQWPVAPLDDTPEAAAEMCRRLGLPC"
     gene            1675..2562
                     /locus_tag="VKQ27_07100"
     CDS             1675..2562
                     /locus_tag="VKQ27_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HLY88735.1"
                     /translation="MDRDLIGYGAHPPDPKWPGGARLAVNFVLNYEEGSEPSVPDGDP
                     ASEWGLTENGMLNPGVKGRDLAAEGMFAYGSRVGFWRIMRLFAERNLPLTVFGCALAL
                     ERHPPAAAAIRAAGHDVCCHGWRWEKHWELTQAEERDRIHRAVASLQQTMGERPLGWY
                     CRYGPSLNTRNLLADEGGFLYDSDAYDDELPYWTSVQGKPYLVVPYSLSTNDSKFGRG
                     TFATGDDFFTYCRDAFDFLYREGGRMMSIGLHMRIMGHPARAAGLERLLDHISRRPGV
                     WVTRRIEIARHWAATHPAA"
     gene            2559..3512
                     /locus_tag="VKQ27_07105"
     CDS             2559..3512
                     /locus_tag="VKQ27_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019647075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY88736.1"
                     /translation="MMQEPQRILIVKLGALGNVILSLGPFASIRRHHAGAHITLLTTV
                     PYASWLSQSQWFDTVWTVRRLDWWDLAGWLRLRRRLIDGHFDRIYDLQTSRHTSRYFR
                     LLPRRHRPDWSGIAPGCSLPDRDPDRDRLHDAERQFGQLRQAGIIERGPVDLSWSHAD
                     IGPFCLPDHFALLAPGSAPHRVVKRWPIEHYRALASRLLAHGVTPVVVGTTPEKPLAQ
                     AIDGAIDLTGRTDLAQVTSLARAATVAIGNDTGPMHLIAAAGCPSVVLFSRDSDPALC
                     APCGPSVTTLRRPDLSDLEPASVMDAVAAILPAQAGALVAG"
     gene            3512..4900
                     /locus_tag="VKQ27_07110"
     CDS             3512..4900
                     /locus_tag="VKQ27_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88737.1"
                     /translation="MADQPERPPHLTAPDGVPLPQRYWAILTVALGLTLAVLDGAIAN
                     VALPTIARDLDASPASSIWVVNAYQIAVTISLLPLASLGDIYGYRRVYQYGMIVFTIA
                     SLGCALSDSLLTLTIARIVQGFGAAGIMSVNGALVRFIYPRRLIGTGIGLNATIGSIA
                     SAVGPTVASAILSVATWPWLFAVNVPLGLIAIPVAFRALPVTHSSGVRFDGLSALWSA
                     LTFGILIIAISGMGHGGSASLGGAELCIAAVFGFVLVTRQLSLSSPMLPVDLLRIRLF
                     ALSVATSITGFLAQSMALVSLPFLFENTLGRDPVQTGLLLTPWPLSVAVIAPLTGRLA
                     DRYPVGLLSGCGLAVMAGGLALASLLPAHPSAFDIVWRMMICGLGFGFFNTPNNRAII
                     ISAPPTRAGAASGMQATSRLLGQSTGAAMVALMFGVFPISGTRESLVFAACAAGAAAL
                     VSFSRLTPVAQA"
     gene            complement(4903..5475)
                     /locus_tag="VKQ27_07115"
     CDS             complement(4903..5475)
                     /locus_tag="VKQ27_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLY88738.1"
                     /translation="MFAQNIGHVVKFLGLNEATGPVLTPEQLIGRLDTFLDAGLRMIS
                     QMPDERLSTEVPNRPRSYRVLGHHIFRIAETFLEVANGATLEYGKLTATPPEGMQTTA
                     AIAAYGTDVRQRLMAWWAAKPDKSARETVQTYYGPQLLHEYLERSTWHVGQHVRQWNM
                     LLGMAGIEADHPPGPADFANLPMPSQVWDG"
     gene            5452..>5677
                     /locus_tag="VKQ27_07120"
     CDS             5452..>5677
                     /locus_tag="VKQ27_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88739.1"
                     /translation="MADILREHVLVAARKHRDRPGSQPPQLIEPGGVFQHIDGLELDR
                     TDREKLFEFQATRSSRLPEHLQRGRVHNQLS"
BASE COUNT          885 a         2112 c         1736 g          944 t
ORIGIN      
        1 ggatgaacag ctgcccggtg aaggccggca gcttgaaccg ccggcggacc tcccggctgt
       61 cgatggaaaa tccgcagggc ccggccggca cttcctgctg ccacgtcgcc agcgcgccac
      121 cgtccgcatc aaacagccgc agccacagcc ggatcgcgcc accgccatag cccgaccagt
      181 agttggccga cacgagccgc gtcgaaagcc cgtccgcatc gcggaagaac gcgaagttgg
      241 tggcgaagtt ctgcttgtcg agatagcgcc cggaattggt caacagctcc gccggcaacc
      301 gcgcctcatc cagcgtcagt accgcggcac cggcaggcag cagatccgcg atgcgcgcag
      361 acgcgcgcct cgcatcgaac gcagcgatca acacggtgcg cgccttgcag ttcgccaggt
      421 ccgtcagcgg cagcgcctgc ggccccactg cagtcacatc ttgcacgtac cgcgtatgcg
      481 ccggcgcatc ggggtacagc gcgagtagcg ccgcggcgat gccctccgga tcatacagcg
      541 ccacctgccc ttcaagtcgt gtatacagcc tggcgatcgc ctcggccgcc agcggatgcg
      601 acagcgcctt gtacagcacg ttgccgccgg cgcgattatc gaatgtctgg atgttcagca
      661 tggcagcccc aaccggcggc acatctcggc ggccgcctca ggcgtatcgt cgagtggcgc
      721 caccggccat tgttcgaagc ggccggcgaa ttcgcgcacc cggccctcag ccagcatgca
      781 gtcgatgaac aggccgtttc gccgcgatcg gccaggaagc ttcgccagca gcaccggcgc
      841 ggacgtcgcc gccgcctccg agaccatgga gacactgtcg accgtcacca cgatcgcatc
      901 ggccagtgcg agcatgccga aataaggatt gtcgcccgcg ccatcccaca cgtaggcgcc
      961 gagcgggatc aatgcctcag ccagcacgcg taccgccgcg ggatcggtgc gccgcgatgg
     1021 cgtcaccgcc acgcccacgc cgtcccgctg catcatcgcg gcgagctgtg acgcgagctc
     1081 ccccgccacc tccgcatcca gccggaagcg gccattgctg ccaccgacca gcacggccac
     1141 cagcggccgc ttcaggtgtt cgaggcgtgg cgcccacacg ctccccgcct cggcaagccg
     1201 cgccggcgtc acccggtgca gcgccgtgcg ggtcaccacg acgttgggcc ccgtcagctc
     1261 atcgtgccgg ttcaccacca ccacatcgaa gcgcctgggg tccatccgag ggtgctgcac
     1321 ctgcaccacg gggcggccac ctcgccgcaa cgcagccccc accgccgcgg cgacgcctcc
     1381 gcagccgatg accagctccg gcaaaggcgc acgcacggcg tcgcgcaccg cggccagcgg
     1441 tgccggccaa agcgacgccg gcacccactt ccagggcgcc cgtggcacaa gcacgcgcaa
     1501 ttccggcgcc aggcaggcgg cctcggcaag gccaagcgcc tgcgcctgca agccggcata
     1561 gccctcgctg agaatccacg atgtggcggc ggccgacata taccggcttt tcaacgcgta
     1621 tcaccggcca gtcaaccagt ggccagcgcg atccgatcca ggcattctgc cgccatggac
     1681 agggacctga tcggctacgg cgcgcatccc cccgacccga aatggccggg cggtgccagg
     1741 ctcgcggtca acttcgtgct gaactacgag gaaggttcgg aaccttctgt tccggatggc
     1801 gatccagcat cggaatgggg actgaccgag aacggcatgt tgaacccggg tgtgaaaggc
     1861 cgcgacctcg ccgccgaggg catgttcgcc tacggcagcc gcgtcggttt ctggcggatc
     1921 atgcgcctgt tcgccgagcg caacctgccg ctcaccgtgt tcggctgcgc cctggcactg
     1981 gaacgccatc cccccgccgc cgccgcgatc cgcgccgccg ggcacgacgt gtgctgccac
     2041 ggctggcgct gggaaaagca ctgggaactc acccaagccg aggaacgtga ccgcatccac
     2101 cgcgcggtgg cgtccctcca gcagaccatg ggcgagcggc cactcggctg gtactgccgc
     2161 tatgggccca gcctcaacac ccgcaacctg ctcgctgacg aaggtggatt tctttacgat
     2221 tcagatgctt atgatgatga gcttccttat tggacgagcg tccagggcaa gccgtacctg
     2281 gtagtgccct acagcctgtc taccaacgac tcgaaattcg gccgcggcac cttcgccact
     2341 ggcgacgact tcttcaccta ttgccgcgac gccttcgact tcctgtatcg cgagggcggc
     2401 aggatgatgt ccatcggcct gcacatgcgc atcatgggcc atcccgcgcg cgcagccggg
     2461 cttgaacggc tgctggacca catcagccgc cggccagggg tctgggtcac ccggcgcatc
     2521 gagatcgccc gccactgggc cgccacgcat cccgcggcat gatgcaggag ccgcagcgga
     2581 tcctgatcgt gaagctcgga gcgctgggga acgtcatcct ctcgctcggc cctttcgcgt
     2641 cgatccgccg tcaccacgcc ggcgcccaca tcactctgct gacgaccgtc ccgtacgcct
     2701 cctggctgtc gcagtcacag tggttcgata ccgtgtggac cgttcggcgc ctcgactggt
     2761 gggacctcgc cggctggctg cgcctccgcc gccgcctgat cgacggacat ttcgaccgca
     2821 tctacgacct gcagacctca cgccatacca gccgctattt ccgcctgctg ccgcgccggc
     2881 acaggcccga ctggtccggc atcgcccccg gttgctcgct gcccgaccgc gatcccgacc
     2941 gcgaccggct gcatgacgcc gaacgccagt tcggtcagct tcgtcaggcc ggcatcatcg
     3001 aacgcggacc cgtcgacctc tcgtggagcc atgccgacat cggaccgttt tgcctgcccg
     3061 accacttcgc gctgcttgct cccggcagcg cgccgcatcg cgtggtcaag cgctggccga
     3121 tcgagcacta ccgcgctctg gcaagcagac tgctggcgca tggcgtaacc ccggtggtgg
     3181 taggcaccac gccggagaag ccgctggcac aggcgatcga cggcgcaata gacctgacgg
     3241 gccgtaccga tctcgcccag gtcaccagcc ttgcgcgcgc cgccaccgtg gcaatcggca
     3301 acgacaccgg ccccatgcac ctgattgccg ccgctggctg cccctcggtc gtgctgttct
     3361 cccgcgactc agaccccgcc ctgtgcgcac cgtgcggccc cagcgtgacg acgctgcgcc
     3421 gccccgacct gtcggatctc gaacccgctt ctgtgatgga cgctgtcgcc gccatcctgc
     3481 ctgcccaagc cggcgctctg gtcgccggct gatggcagac cagccagagc gcccgccgca
     3541 tctcaccgcc ccagacggcg tccctctccc ccagcgctac tgggccatcc tgaccgtcgc
     3601 actcggcctg acgctcgcag tgctcgatgg cgccatcgcc aacgtcgccc tgccgaccat
     3661 cgcccgcgat ctggatgcca gcccggcatc atcgatctgg gtggtgaacg cctaccagat
     3721 cgctgtgacg atatcgctcc tgccgctcgc ctcgctgggt gacatctacg gctaccgtcg
     3781 cgtctaccag tacgggatga tcgtgttcac catcgcatcg ctgggctgcg cgctctccga
     3841 ctcgctgctc accctcacca tcgcgcgcat cgtccagggc tttggcgccg ccgggatcat
     3901 gagcgtcaac ggcgccctcg tccgcttcat ctatccgcgc cggctcatcg gcaccggaat
     3961 cggactgaac gcgacgatcg ggtcgatcgc gtccgccgtc gggccgacgg tcgcctccgc
     4021 catcctgtcg gtcgccacct ggccctggct gttcgccgtc aatgtcccgc tcggactgat
     4081 cgcaatcccc gtcgccttcc gtgccctgcc ggtgacccac agctccggcg tccggttcga
     4141 cgggctcagt gcgctctgga gcgcactcac cttcggcatc ctgatcatcg ccatcagcgg
     4201 catgggccat ggcggcagcg ccagcctggg cggcgccgaa ctctgcatcg cggcggtgtt
     4261 cggcttcgtc ctggtgacgc ggcagttgtc gctcagctcg ccgatgctgc cggtcgacct
     4321 gctgcgtatc aggctgttcg ccctctccgt cgcgacgtcg atcaccggtt tcctcgccca
     4381 atcgatggcc ttggtgtcgc tgccgttcct gttcgagaac acgctcggcc gcgacccggt
     4441 ccagaccggc ctgctgctca cgccatggcc tttgtcggtc gcagtcatcg ccccactgac
     4501 cgggcggttg gcggaccgct atccggtggg gttgctcagc ggctgtggcc tggcggtcat
     4561 ggcgggcggc ctggcactcg ccagcctgtt gcccgcccat cccagcgcat tcgatatcgt
     4621 ctggcgcatg atgatctgtg gccttggctt cggcttcttc aacacaccga acaaccgggc
     4681 gatcattata tccgccccac ccacacgtgc cggcgcggcg agcggcatgc aggccacgtc
     4741 tcgcctcctg ggtcagagca ctggtgccgc gatggtagca ctgatgttcg gcgtgtttcc
     4801 gatcagcggc acgcgcgagt cgctggtgtt cgcggcctgc gcggctggtg ccgcggcgct
     4861 ggtgagcttc tcgcggctta cgccggtggc acaggcctga gcctagccgt cccagacctg
     4921 gctcggcatc ggcaggttgg caaagtccgc cgggccgggg ggatggtccg cctcgatgcc
     4981 cgccatgccc agcagcatgt tccactgccg cacatgctgg ccgacgtgcc acgtcgagcg
     5041 ctccaggtat tcgtgcagaa gctgtggtcc gtaataggtc tgcacggtct cgcgcgccga
     5101 cttgtccggc ttggccgccc accacgccat cagccgctgc cgcacgtcgg tgccataggc
     5161 agcgatggca gccgtggttt gcatcccttc cggcggcgtc gcggtcagct tgccgtactc
     5221 cagcgtggct ccgttggcca cttccaggaa tgtctctgcg atgcggaaga tgtgatgccc
     5281 cagcacgcgg tagctgcgcg gccgattcgg cacttctgtc gagagccttt cgtcgggcat
     5341 ctgcgagatc atgcgcagcc ccgcgtcaag gaacgtgtcg agccgcccga tcagttgctc
     5401 gggcgtcagc accggcccgg tcgcctcatt cagcccgagg aacttcacga catggccgat
     5461 attctgcgcg aacacgtact cgtcgccgcg cgaaagcacc gggacagacc gggctcccag
     5521 ccgcctcagc tcatcgagcc cggcggggtc ttccagcaca ttgatggact cgaactcgat
     5581 cgcacggacc gagagaaact ctttgagttt caggcaactc gttcatccag gctgccagaa
     5641 caccttcagc gcgggcgcgt ccataatcag ctctcac
//
LOCUS       DATGEM010000228         5331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_947234, whole genome shotgun sequence.
ACCESSION   DATGEM010000228 DATGEM010000000
VERSION     DATGEM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947234"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VKQ27_07125"
     CDS             <1..194
                     /locus_tag="VKQ27_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY88740.1"
                     /translation="AIETVFAYPGLGQLLMFALQRQDLPVMQAAILILTGVFCISNLI
                     ADLLYGVVNPRIRYGGAVG"
     gene            187..1059
                     /locus_tag="VKQ27_07130"
     CDS             187..1059
                     /locus_tag="VKQ27_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88741.1"
                     /translation="MADAAALDTDTSTDTASRRARLPISLKVGAAIVLALLIASALAQ
                     VIAPYPFDEMHIADRFSPPSLQYLAGTDEYGCDVFSRVLIGSQLSLLLGVSATLISLG
                     LGVPLGLLAGYRRGLVDEVIMRVLDILLAFPPVMLVLLILAVTQPGLIKTAFAIGLLS
                     APPIGRVTRGVTLDLMSGEFIEAARARGERLGYMLWRELLPNVWPVLMVEASLRVVFA
                     ILLGAVLSFLGFGVQPPSADWGLMISAGRDYVDEAPWISLAPGIAMSITVIGISLLGD
                     GLREVLDPRLSGRR"
     gene            1056..3050
                     /locus_tag="VKQ27_07135"
     CDS             1056..3050
                     /locus_tag="VKQ27_07135"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88742.1"
                     /translation="MNAEVEIPPPLLDVRDLTVRYGHEDRSAAVRQVSFHLQPGEALG
                     IVGESGSGKSSIAGAILDFLGPAARISGEIRFGGRDLLKLPRKQHRAVLGHKIGSVFQ
                     DPFSALNPALRIGRQIAEPLIRHLGMPPADAARRTVDLLREMGIHRAEDVARAYPHQL
                     SGGMKQRALIAAAVACEPPLLILDEPTTALDVTVEAQILRLLGRLRQQKRVSLLFISH
                     NLGVVRRLCDRVAVMYASQFVELGTMRGVLEHPAHPYSKGLLASRPPLQAMPRGNRMP
                     SIQGQMPIAPRPEEGCVFAPRCPFAEPRCAEQPQSMQAAMQGNLARCWKADVAGEWPR
                     QAAEVLDHEAFRAGDALVNVTHLRKTFSVSGRLAALLRRRHDHAAVDDVSLSISPGEV
                     LGLVGESGCGKSTLGRLTLRLLEQSAGSVEFDGADVSLVAGADLAEFRKQAQIVFQNV
                     GSSLNPRLSVGEALERPLVLFGLAKPRERRRRVEELLEMVRLPLSYRLRYPHQLSGGE
                     RQRVAIGRALATEPRFIVCDEPVSALDVSVQAAIVNLLADLRDQFGLAYLFISHDLAV
                     VAQLSDRIAVMYRGRICETGTPAGLLAGPRHPYTRMLLAAVEDTTIDEAPADEAGATA
                     LNGCAFAARCPCRLPDICDTTPPSLRAVGTSHEVACHNLQ"
     gene            3068..4378
                     /locus_tag="VKQ27_07140"
     CDS             3068..4378
                     /locus_tag="VKQ27_07140"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493858.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HLY88743.1"
                     /translation="MSAAPFHLGWFMNFTAGEWDHPLSVGGSPWDGRFYVDMAQALER
                     ACFDYIMIEDTLMVSETFGGSAEGGMKHAQFVPKHDPSPLAAVISAATQRLGVVATLS
                     TLAYPPFMLARLCSTLDHIAGGRFGWNIVTSGEELAAQNFGLEHLPPRQQRYDMADEY
                     VDLVCQLFNSWDADAVVMDRATGTYADYRKVRPIHFEGKYFKCRGPLNTVRSPQGQPV
                     FVQAGGSPRGRAFAARHADSIIAVANGIEGMKQYREDVRAHAEKWGRDPDHAKVLFLV
                     YPFLGETDAVAHAKYQRMTASPAFIERALVGISAITDIDFSKFPLDEPLPHLTTNGEQ
                     GSLDKFAQWGSGKTLRQLAMDRFEAVEPELSFVGSPDTVAERMRRVMEAVGGDGFLIS
                     TPFQRTSRRYIIEVTEGLIPALQRRGLTRSTYIGRTLREVLREF"
     gene            4531..4842
                     /locus_tag="VKQ27_07145"
     CDS             4531..4842
                     /locus_tag="VKQ27_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY88744.1"
                     /translation="MATKIRRSFTDEFKQEAVSLLASSDRPLTQVARELGIQPSMLRH
                     WRDDRQANCTVAGAAGSLASGGTSAEHAEMRRLEKELARAQMERDISKRAIGIFLGPP
                     K"
     gene            4981..>5331
                     /locus_tag="VKQ27_07150"
     CDS             4981..>5331
                     /locus_tag="VKQ27_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLY88745.1"
                     /translation="MIEEIRRVYGDSRGRYGRPRVHAALRAEGRQIGRNRVARLMHHH
                     GICARRKRRFCRTTDSSHEFPLAPNLLGRKFTASAPDRVWLADITYIPTAEGWLYLAV
                     VLDMFSRRVVGWAMD"
BASE COUNT          905 a         1669 c         1733 g         1024 t
ORIGIN      
        1 ttgcaattga aaccgtgttc gcctatccag gtcttggcca actgctgatg ttcgcgctgc
       61 agcgccagga tctgccggtg atgcaagcgg cgatcctgat cctgaccggc gtcttctgca
      121 tctccaacct catcgccgat ctgctctacg gcgtcgtcaa tccgcgcatc cgctatggtg
      181 gcgcagttgg ctgatgcggc cgccctggat accgacacca gcaccgacac cgcctcgcgg
      241 cgcgcgcgtc tgccgatctc actcaaggtc ggtgccgcga tcgtgctggc actgctgatt
      301 gcctcggcgt tggcacaggt gatcgcgccc tatccgttcg acgagatgca cattgccgac
      361 cggttctcgc cgcccagcct ccaatatctc gccggcaccg acgagtacgg ctgcgacgta
      421 ttcagccgcg ttttgatcgg ctcgcaactg tcgctgctgc tcggcgtcag tgcgaccctg
      481 atcagcctcg gcctcggcgt accgctcggc ctgctcgctg gatatcgccg cgggctggtc
      541 gacgaggtga tcatgcgagt actggacatc ctcctggcat tcccgccggt catgctggtg
      601 ctgctgatcc tcgcggtcac ccagcccggc ctgatcaaga ccgcattcgc catcggcctg
      661 ctgtccgcgc caccgatcgg acgcgtcacc cgcggtgtca ccctcgacct gatgagcggc
      721 gagttcatcg aggcggcgcg cgcccgcggc gagcggcttg gttatatgct gtggcgcgaa
      781 ttgctgccga atgtctggcc ggtgttgatg gtggaagcga gcctgcgcgt cgtgttcgcc
      841 atcctgctgg gcgcggtgct gagtttcctt ggcttcggcg tgcagccgcc gtccgccgac
      901 tggggcctga tgatcagcgc gggtcgcgac tacgtggacg aggcaccgtg gatctcgctc
      961 gctcccggca tcgccatgag catcaccgtc atcggcatca gcctcctggg cgacgggctg
     1021 cgcgaggtat tggatccacg gctgagcggc cggcgatgaa cgccgaagtg gagatccccc
     1081 cacctctgct cgatgtccgc gacctcaccg ttcgctatgg acacgaagac cggagtgctg
     1141 ccgtacggca ggtttcgttt catctgcaac caggcgaggc gcttggcatc gtcggcgaat
     1201 cgggatccgg caaaagcagc attgccggtg cgatcctgga tttcctgggg ccggccgcac
     1261 gtatcagcgg cgaaatccgc ttcggcgggc gcgacctgtt gaaacttccg cgcaagcaac
     1321 accgcgcggt gttgggtcat aagatcggct cggtcttcca ggatccgttc agcgcgctca
     1381 acccggcgct gcgcatcgga cggcagatag ccgaaccgct gatccggcat cttggtatgc
     1441 cgccggcgga tgccgcccgc cgcacggtgg acctgctgcg cgagatgggc attcaccgcg
     1501 ccgaggatgt tgcccgagca tacccgcatc aactcagcgg cgggatgaaa cagcgcgcgc
     1561 tgatcgccgc cgctgttgct tgcgagccgc cgctgctgat cttggatgaa ccgaccactg
     1621 cgctcgatgt cacggtcgaa gcacagattc tgcggttgtt ggggcgcttg cggcagcaga
     1681 agcgagtgag cctgctgttc atcagtcaca atctcggtgt ggtgcggcgg ctctgcgacc
     1741 gcgtggcggt gatgtacgcc agccagttcg tcgaactcgg caccatgcgc ggtgtgctgg
     1801 agcatccggc acatccctac agcaaggggt tgttggcgtc gcggccgccg ctgcaggcga
     1861 tgccgcgcgg caaccgcatg ccttcgatac agggccagat gccgatcgcg ccgcgcccgg
     1921 aagaaggctg tgtgttcgcg ccgcgttgcc cgttcgccga gccgcgctgt gctgagcagc
     1981 cgcaatcgat gcaggccgcg atgcagggta atctggcacg ctgctggaaa gcagacgtcg
     2041 caggcgaatg gccgcgacag gcagcggaag tgctcgatca cgaagcattc cgggccggcg
     2101 atgccctggt caacgtgaca catctgcgca agacgttctc ggtgagcggt cgtctcgctg
     2161 ccctgctgcg gcgtcgccac gaccacgcgg cggtggacga tgtctcgctg tcgatctcgc
     2221 caggcgaagt actcgggctg gtgggagaaa gcggctgcgg caagtcgact ctcggccgtc
     2281 tgactctgcg cctgcttgaa cagagcgccg gcagcgtgga gtttgacggc gccgatgtca
     2341 gcctggtcgc cggcgccgat ctcgcggaat ttcgcaagca ggcgcagatc gtgttccaga
     2401 acgtcggttc ctcactgaac ccgcgacttt cggttggcga ggcgctcgaa agaccgctgg
     2461 tgctgttcgg cctggcgaaa ccccgcgagc gacgcaggcg ggtcgaggaa ctgctggaga
     2521 tggtgcggct gccgctctcc taccggctgc gctacccgca tcaactcagt ggcggcgaac
     2581 gtcagcgtgt tgcgattggg cgcgcgctgg ctaccgagcc gcgcttcatc gtgtgcgacg
     2641 aaccggtgtc tgcgctggat gtctcggtgc aggccgcgat cgtcaatctg ctcgcggacc
     2701 tgcgcgatca gttcgggctc gcctatctgt tcatctcgca cgacctcgcg gtggtcgcac
     2761 agctttccga ccgcatcgca gtgatgtatc gcggccgtat ctgcgagacc ggcacgccgg
     2821 ccggactgct ggccgggccg cgccatccct atacgcgcat gctgctcgcc gccgtggagg
     2881 acaccaccat cgacgaggca cctgccgatg aagctggcgc gaccgcgctg aacggctgcg
     2941 cgtttgccgc gcgttgcccg tgtcgccttc ccgatatctg cgacacgacg ccgccctcgc
     3001 tgcgcgctgt cggcacatcg catgaggtcg cctgtcacaa tctgcagtaa cgaaaggacc
     3061 ctgaagcatg tccgccgcgc ccttccacct cggctggttc atgaacttca cagcaggcga
     3121 gtgggatcat ccgctgtcgg tggggggctc gccctgggac ggccggtttt acgtggatat
     3181 ggcgcaggca ctggaacggg cctgtttcga ctacatcatg atcgaagaca cgctgatggt
     3241 gtctgagacg tttggcggca gcgccgaagg tgggatgaag cacgcccagt tcgtgcccaa
     3301 gcacgaccct tcgccgctcg ccgcagtgat cagcgccgcc acgcagcgcc ttggcgtagt
     3361 ggcgaccctc tctaccctgg cctatccgcc gttcatgctg gcacggctgt gttcgacgct
     3421 cgaccacatc gccggcggcc ggttcggctg gaatatcgtc acctccggcg aggaactcgc
     3481 ggcacagaat tttggtctgg agcatctgcc accgcgccag cagcgctacg atatggcgga
     3541 tgaatatgtg gatctggtct gccagttgtt caattcctgg gacgcggacg ccgtggtgat
     3601 ggaccgagcg accggcacct acgccgatta ccgcaaggtt cggccgatcc atttcgaggg
     3661 aaagtatttc aagtgccgcg gcccgctaaa taccgtgcgc tcgccgcaag gccagccggt
     3721 gttcgtgcaa gccggcggct cgccccgcgg gcgagccttt gccgcgcggc acgcggattc
     3781 gatcatagca gtggccaacg gcatcgaagg gatgaagcaa tatcgcgagg acgtgcgcgc
     3841 gcacgccgag aaatggggcc gcgatcccga tcatgccaag gtgctgttcc tggtctatcc
     3901 gttcctaggc gaaacggatg ccgtggcaca cgcgaaatat cagcgcatga ctgcttcgcc
     3961 ggccttcatc gagcgtgcgc tcgtcggcat ctccgccatt accgacattg atttttcgaa
     4021 gttcccgctg gacgaaccgc tgccgcatct gacgacgaat ggcgagcaag gatcgctcga
     4081 caagtttgcg cagtggggca gcggcaaaac gcttcgtcag cttgcgatgg atcggttcga
     4141 ggcggtcgag ccggagctgt cgttcgtcgg ctcaccagac acggtagccg aacggatgag
     4201 gcgggtgatg gaagcggttg gtggcgatgg ctttctgatc agcacgccgt tccagcgcac
     4261 cagccgccgc tacatcatcg aggtgaccga aggacttatc ccggcattgc aacgccgtgg
     4321 tctgacccgc tcaacctata tcgggcgaac tctgcgcgaa gtgctgcgcg agttctgacc
     4381 tgccgcacgc cgtattggtc agggtggtcg attaaggcca ggtgctatgg ctttcttagc
     4441 gcctcttgct gtagtgggaa cgcctaaaca atgaccctcc ccggtacagt ggacacctct
     4501 ggtaagcccc tagatggagc gaggtgaccg atggcgacga agatccggcg atcattcacg
     4561 gacgagttca aacaagaagc ggtgtcattg ttggcatcga gtgatcgacc attgacccag
     4621 gtggcgaggg agttgggtat ccaaccctcg atgctgcgtc actggcgtga tgaccggcaa
     4681 gcaaattgca ctgtggcggg ggccgccgga agcttggctt cgggtggcac atcggcggag
     4741 catgccgaga tgcggcgcct ggagaaggag ctggcacggg ctcagatgga acgggacatt
     4801 tcaaaaaggg ccatcggcat attcttgggt ccaccgaaat gagatttcgc tttatccatg
     4861 atcaacgagc gacgttcccg gtgcgcgtga tgtgcaagat gctggacgtc ggcgccagcg
     4921 gctattacgc gtggagggat cgaccggaaa gcatgcgatc gtccaccaac caatcgccag
     4981 ttgattgagg aaatccgtcg cgtatatggc gatagcagag gtcgctacgg caggccacgt
     5041 gtgcatgccg ccctccgcgc ggagggacga cagatcgggc gcaatcgagt ggcgagattg
     5101 atgcaccacc atggcatttg tgcccgccgg aagcggcggt tctgccggac cacagacagc
     5161 agccacgaat tcccgctggc tcccaatctg ctcggccgaa agttcacggc atctgcgccc
     5221 gatcgtgtgt ggctggcgga cattacgtat attccgaccg cagagggttg gctttatctc
     5281 gcggtcgtcc tcgacatgtt ctcccgtcga gtggtgggtt gggcgatgga t
//
LOCUS       DATGEM010000229        13351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1134961, whole genome shotgun sequence.
ACCESSION   DATGEM010000229 DATGEM010000000
VERSION     DATGEM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13351
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134961"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VKQ27_07155"
     CDS             <1..575
                     /locus_tag="VKQ27_07155"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88746.1"
                     /translation="GHLTWDEGHQPKPGEIGFVFQDATLLPWASATDNVYLPLRLAGV
                     ARTEAMPRVHEALRHVGLSGFESARPRQLSGGMRMRVSIARALVTRPRLLLMDEPFAA
                     LDEFTRNKLQEDLLALWQELGCTVVFVTHSIYEAAFLARRIVLMTPRPGRIDRELPSP
                     LHPGAEARLDPAYAALVAQITRDLQQALAA"
     gene            572..1321
                     /locus_tag="VKQ27_07160"
     CDS             572..1321
                     /locus_tag="VKQ27_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY88747.1"
                     /translation="MKYAPALFGLAALVVWEVAVRITDVPSYLVPGPIAIIQAFFAEP
                     MLLLVSLGSTLLVTFAALLVAAALGVALAIAIASNRLLAAAIQPWAIVLQVTPIVAIA
                     PLIIVWVGNPFASLVVCATIVAFFPVFANTAAGLASAPPELIDLFRLYGATRTTTLRL
                     LRLPAALPYFLAGLRISGGLALVGAVVAEFVAGSGGFASGLAYRILEAGYRLQVPRMF
                     AALVLLSLAGIAINFGLGALGNFILRRRDDA"
     gene            complement(1336..2649)
                     /locus_tag="VKQ27_07165"
     CDS             complement(1336..2649)
                     /locus_tag="VKQ27_07165"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY88748.1"
                     /translation="MTNAIESEQLTRVGPGTMMGDLMRQYWLPAVKSSEVVAGGDPLR
                     LMLLGERLIAFRDQSGRIGVMDHRCPHRCASLFFGRNEPGGMRCVYHGWKFDADGNCI
                     DMPNLTPEQDFRQKVHAKAYKVCERAGVIWVYMGVRAEAPPMPEIEATLLPESELSIT
                     FTQRECNWLQALEGDIDTSHFSWLHVGSVRPEQVDPSNWLMYQVNNRAPDYHVSDTDW
                     GTMYCAFRPAEGDRTYWRFAHFAFPFWTFIPQGDFMDRVIARAWVPMDDTHVMFVGFS
                     WKQASRTAALAGGEPIPGSTPAPDMMPNSTDWFGRFRPVQNAANDYLIDREAQRDDVI
                     YTGITHIAMQDQAITESMGEIVDHSFEHLAPSDQMITRTRRRLLMAARALHEKGAAPP
                     CVDQPDIFRQVRSGEAVLAADDWQAAYRDRMGQVMRPTGWKVAAE"
     gene            2836..4230
                     /locus_tag="VKQ27_07170"
     CDS             2836..4230
                     /locus_tag="VKQ27_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88749.1"
                     /translation="MLEHLEQQQKLTRNQWRIIATANLGDMLDFFDFFLIGYVLAFII
                     GSWKLTFGQSAVILLSSGLGAVPGAFFWGWMADKFGRRTIFMATALNVSIATGIMAFT
                     PDQDALIPGWFFLAFFRFFVGFGNAGLIAVDIPLVQEFVPAYKRGWVSGLTTVLLPAG
                     NLMGAVSGAFLAPLIGWRGLFLIGLAPALLVLMIRYWVPESPHWLLRRGRMEEARRSL
                     AWALQVDPQQIALPTSVPDAVKPLPWTELFRYPRSIAAGCLTAISQTGGVGLLLWIIA
                     LFVLVLRITPAEASYLMIYVSILGILGRLFCSYLSDAIGRKASGAIIGYAGAITMALA
                     GYMNSVWLGGVSMFFVLVMAQRFFGDGSYAIIGPYIAEVWPARLRASGMGLAYGVGNI
                     GKIIGPLGLALIIGSSNYVSPKATLDAIVPAFLYLAFWYALAATAFAFLAVETKGRTI
                     DEIDATLVKAPAAE"
     gene            complement(4242..4802)
                     /locus_tag="VKQ27_07175"
     CDS             complement(4242..4802)
                     /locus_tag="VKQ27_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HLY88750.1"
                     /translation="MDAMELLVARESATKLTSPGPDQEALERIFQSALRAPDHGRLRP
                     WRFIVVPEERRERFGEIMADCLRRQQPDASADMLQRERDKAMRAPVIVVAAATVQRGH
                     KIPEVEQLVSAAAAAENIMLAANALGYGAMWKTGAPAYDSGVKQAFGLDADNDIVGFL
                     YLGTQVGGGSPAARPTVKDLVSVWQG"
     gene            complement(4816..5853)
                     /locus_tag="VKQ27_07180"
     CDS             complement(4816..5853)
                     /locus_tag="VKQ27_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY88751.1"
                     /translation="MIFRQLFDATSGTYTYLLASRRGGEALIIDPVLEKVDRYLQLIR
                     ELDLKLVKAIDTHLHADHITGLGALRDRTRCITVMGEQSSVDVVSMRIGEGDRLGIEG
                     ISLDVAYTPGHTDDSYSFIMPDRVFTGDTLLIRGTGRTDFQNGDPRAQYESIFNRLLR
                     LPDETMVFPAHDYKGDTVSTIGEEKRYNPRLQVKSVDEYMALMNGLNLSNPKMMDVAV
                     PANMRQGLAQQEIARRGWSMTADEAKALIGNRDVALIDLRERTERERHGVIPGSLHAP
                     YPDLQENIQDGGMLHELATATSKRLVFYCAFGERSAMAVQAAQDAGLASACHIHGGID
                     AWKKADGPLAH"
     gene            complement(5911..6795)
                     /locus_tag="VKQ27_07185"
     CDS             complement(5911..6795)
                     /locus_tag="VKQ27_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88752.1"
                     /translation="MNTVERIAAIAAEPRVSRPSDNFARYVKARNRRVRLRRFIRGAT
                     GVVLILLAWQVMSQVLDLELLLPEPLTVLRNVVATLTVSDPHWLYGPNIYVHLWHSFL
                     RAMAGFVSAAVLAIPLGLALGRISWLREFMMPAIQGVYPIPGIAWIPLAILWFGLGDA
                     AVVFVVFATVFFPLLFNAEAGARQISPTVLDAGRCFGARGVSFFFRIVLPATVPFIIT
                     GLRISLGGAWRMIVAGEMLASQDGIGYLLMESRFQFRAVDLMTAMILVAVVGYATEKV
                     IVGTIEKRTIEKWEVVSS"
     gene            complement(6792..7802)
                     /locus_tag="VKQ27_07190"
     CDS             complement(6792..7802)
                     /locus_tag="VKQ27_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88753.1"
                     /translation="MKRRGLLALPAVAVLSGLWRSRAFAADKIKVGMLKPNIVTVIYW
                     IAVKTGAFAKNGLEIAEYPVPSGQTAAGIEQLLRNDINFYLGASGEVAHANSRYLEAG
                     KALPIALIEGGISAGTFFVMRNDLKGKSLDELRQMPLKIGLSNPSSFHLIMFRAFLAT
                     KKLTTDDFKWQFLTVGGPEMLPALVSHQLDGFMHDALTTTLALRANAGFEFMSSNRGD
                     FGQEASLVPGTGVCGNMQFIKAHADVARRFTQALRDAGASYATSPRAEMVAIMAEWSR
                     QDPAVIEAMYDRFDPRVGLTAAAANAWWDILGRAMLARGEISQNMTVNDVFDLDYVQQ
                     KS"
     gene            complement(7859..8059)
                     /locus_tag="VKQ27_07195"
     CDS             complement(7859..8059)
                     /locus_tag="VKQ27_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88754.1"
                     /translation="MCGEGGALSCQRAVQRAWRDLCRLGDDEVSGFRACTTLYLIRHP
                     VVSPREARDLVAEWLDHAECDS"
     gene            complement(8065..9222)
                     /locus_tag="VKQ27_07200"
     CDS             complement(8065..9222)
                     /locus_tag="VKQ27_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88755.1"
                     /translation="MARGGYKFFDTDAHVGPYVDVLETYLSADDKRRLEAWSQYRSSS
                     KNGHVSYNKGQRRYQRRLYSETTEAAPGGYMAGFTGVKRERAISPDVDRSSAARIADM
                     DFEGADVNFMLPSGWFGTFTAGDDVALEMGMYRAFHRWMKDYCAPYSDRLGGVVLVSS
                     RDVQGSVEEMRRAGRERWAWGVLVYAPYGTPIDLPSLDPIWAEAQELNLSAVLHTFTV
                     MPPYAPGGLDTWDNLWLQRSAAHPWCGMRNMAALIGSGLLDRFPRLRLGCLEAGHGWL
                     PFWMARLDEHMETIRTALPPDLKHHPSEYVMSGRYYQSIEVSEGEKVTNMVADIVGEN
                     VLMYASDYPHGESAFPESTNIVLGWKHMSEDRKRKLFWENPVKFYARSGLV"
     gene            complement(9235..10206)
                     /locus_tag="VKQ27_07205"
     CDS             complement(9235..10206)
                     /locus_tag="VKQ27_07205"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HLY88756.1"
                     /translation="MALPPSDGARASTRLKQLIHRKGQVLSVMHPPTAALARVMERGG
                     CEALFVGTSAVVGGYTGMADVGTATMTECVKIAEWIANSVSTPVIIDGDTGHGGIMAV
                     RRLVRECIHAGIAGIRIDDQPIEGKRRTQSAGLEVVPLEQAIVRYRAAVDMKNELDPD
                     FVVMAQCYARDAANGGLEETLRRLAAYHREAGVDWVQLESPHSVDEIRQARAAVTCPF
                     SFMKGKLPRYLSLDEHLALGATIAWLPSFSHHVIWAAMSDFMEAFSAQGIGAWEAFVE
                     SRKQHPYTIPEVPADGEGLDKQRELEERYLSAAALEKYRRSSGGALS"
     gene            complement(10187..10993)
                     /locus_tag="VKQ27_07210"
     CDS             complement(10187..10993)
                     /locus_tag="VKQ27_07210"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88757.1"
                     /translation="MTGAATVIAEPSIAPPVIEATDVDHFYEGQLGWNHALDHLTLTI
                     RRNEFLCILGPSGCGKSTFLKIVAGLMHPTNGRVLLNGNPIKGPGLDRGVVFQDPALF
                     AWLTVIKNVEFGLLMGGWKKTAARERAMHVLEIVGLARQASLYPFQLSGGMKHRVAVA
                     RAWALTDAALLLMDEPFSAIDAINRMTLQDHLVNTWLTEQRTVLYVTHDLEEAVYLAD
                     RIVILRPSPGRLAADIRNDLPRPRDRHSAEFLAVKSTIMQEMQRHGVATE"
     gene            11132..11917
                     /locus_tag="VKQ27_07215"
     CDS             11132..11917
                     /locus_tag="VKQ27_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLY88758.1"
                     /translation="MGIAASGSERYSVSTVERALEVLRAFTYRQPELSLTDIAAATGL
                     HKATVFRLLATLCNGGMMLKNPRTGLYRLGYGIIAMSEIAKTTTGFVSQARPFMRQIR
                     DELNETVYIAVRVGDDRINLDQLEGIRDFRRVVTLGKPSPLYVGSTSHVILAALPDDE
                     IAAYIARTEFVPPFPGARIDAQTLRRAITDTRRNGFAETHNKRIDEGASISSLVRGAG
                     NEIMGALTVSIPIGRYTNTVRTRTIEFVTGAASALSGQLGASP"
     gene            complement(11923..12093)
                     /locus_tag="VKQ27_07220"
     CDS             complement(11923..12093)
                     /locus_tag="VKQ27_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HLY88759.1"
                     /translation="MANASINWTNSVSTDPSAPGQSGLSIKTTAVPEPGSLLLLGMGL
                     IGTVAAMRRVRG"
     gene            complement(12162..12473)
                     /locus_tag="VKQ27_07225"
     CDS             complement(12162..12473)
                     /locus_tag="VKQ27_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88760.1"
                     /translation="MQGPVTNNFCLTIKSSGYQWTPSNAGVVNPIYKVGAVNKTFTKS
                     DFPYGPETAKPGPGNGFVLPFYSGQNINDPSTASYLMFVDLDVGNDNIRSSIDSGGAK
                     V"
     gene            complement(13045..>13351)
                     /locus_tag="VKQ27_07230"
     CDS             complement(13045..>13351)
                     /locus_tag="VKQ27_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY88761.1"
                     /translation="ALRYVVHGTPQLMGHCYCADCRKACGSGFLPFIGIASTAVHFTG
                     ATRSFTSRSASATDAVRNFCAVCGSLVFGGVVGKDTSHTIYAGTLGDPTLFMPSIAI"
BASE COUNT         2473 a         4590 c         3791 g         2497 t
ORIGIN      
        1 ccggtcacct gacctgggac gaaggccacc agccaaagcc aggcgaaatc ggcttcgtct
       61 tccaggatgc cacgctcctt ccgtgggcca gcgccaccga caatgtctac ctgccgctcc
      121 gcctcgccgg cgttgcgcgc accgaggcca tgccccgggt ccacgaagcc ctccgccacg
      181 ttggcctgtc cggtttcgaa tcagcccgtc cccgccagct ttccggtggc atgcgcatgc
      241 gcgtctcgat cgcgcgcgca ctcgtgacac gtccgcgcct gctcctgatg gacgaaccct
      301 ttgccgccct cgatgagttc acccgcaaca agctgcagga agacctgctc gcactgtggc
      361 aggaactcgg ctgcacggtg gtcttcgtca cgcactcgat ctacgaggcc gccttcctcg
      421 ctcgccgcat cgtgctgatg acacctcgtc ccggacgcat cgaccgcgag ttgccatcgc
      481 ctttgcatcc cggcgcagaa gcccgcctcg atccggccta cgccgctctg gtcgcccaga
      541 tcacgcgcga cctgcaacag gctcttgccg catgaaatat gcccccgccc tcttcggcct
      601 ggccgcactc gtggtgtggg aagtcgctgt gcgcatcacc gatgttccca gctatctggt
      661 ccccggcccc attgcgatca tccaggcctt cttcgccgaa cccatgctgc tgctcgtgtc
      721 gctcggctcc acgctgctgg tgaccttcgc cgcacttctc gtcgccgcag cactcggcgt
      781 cgccctggca atcgcgatcg cctcaaaccg cctgctggca gcagcgatcc aaccctgggc
      841 catcgtgctt caggtcacgc cgatcgtcgc aatagcaccg ctgatcattg tctgggtcgg
      901 caatccattc gcatctctcg tcgtatgtgc aaccatcgtc gcgttcttcc cggtattcgc
      961 caacaccgca gccggtctcg catccgctcc tcccgaactg atcgacctgt ttcgcttgta
     1021 tggcgctact cgaaccacca cattgcgact gctgaggctt ccggctgccc tgccctactt
     1081 ccttgcaggt ctgcgcattt ccggtgggtt ggcattggtc ggcgccgtag tagccgaatt
     1141 cgtcgccggc tccggcggct tcgcctcagg tctcgcatac cgcatcctcg aagctggcta
     1201 tcggctgcaa gtgccgcgga tgttcgccgc actggtgttg ttgtcactcg cgggcatcgc
     1261 gatcaacttc ggactgggcg cgctcgggaa cttcatcctg cgacggcgcg acgacgcgtg
     1321 acgccgtggc ggcggttact ccgccgccac cttccatccg gttggccgca tcacctgccc
     1381 catccggtcg cgatacgccg cctgccagtc atcggcggcc agcacagcct cgccgctgcg
     1441 aacctgccgg aagatgtcgg gctgatcgac gcaagggggc gccgcgccct tctcgtgcag
     1501 cgcccgcgcc gccatcagca accgccgccg cgtgcgggtg atcatctgat cgcttggcgc
     1561 cagatgctcg aacgaatggt cgacgatctc acccatgctt tcggtgatcg cctggtcctg
     1621 catcgcgata tgcgtgatcc ccgtatagat cacgtcgtcg cgttgcgcct cgcgatcgat
     1681 caggtaatcg ttcgccgcat tctgcaccgg ccggaaccgg ccgaaccagt cggtggagtt
     1741 cggcatcata tcgggcgccg gcgttgaccc agggatcggc tcaccacccg ccagcgccgc
     1801 tgtccgggac gcctgcttcc acgagaaccc cacgaacatg acatgcgtgt cgtccatcgg
     1861 cacccaggca cgcgcgatca cgcggtccat gaaatcgccc tgtggaatga acgtccagaa
     1921 cggaaaggcg aaatgcgcga accgccaata cgtccgatcg ccctcggccg gccggaacgc
     1981 gcagtacatc gtcccccaat cggtatcgga cacatgatag tcgggggcgc gattgttcac
     2041 ctggtacatc agccagttgc tcggatccac ctgctccggc ctcaccgatc cgacatgcag
     2101 ccaactgaaa tgcgaggtat cgatgtcgcc ctccagcgcc tgcaaccaat tgcactcccg
     2161 ctgggtgaag gtgatggaaa gctcgctctc gggcagcaac gtcgcctcga tctccggcat
     2221 cggtggcgcc tccgcacgga cgcccatgta gacccagatc acccccgcgc gctcgcagac
     2281 cttgtaggcc ttcgcatgca ccttctgccg gaaatcctgc tccggcgtca ggttcggcat
     2341 gtctatacaa ttgccatccg cgtcgaactt ccagccatga taaacgcagc gcatgccgcc
     2401 cggctcgttc cgtccaaaga acagcgacgc gcagcgatgc ggacagcgat gatccatcac
     2461 gccaatacgc cccgactggt cacgaaacgc gatcagtctc tcccccagca gcatcagccg
     2521 cagcggatcg ccacctgcca ccacctccga cgacttcacc gccggcagcc agtactgccg
     2581 catcagatcg cccatcatcg tccctggccc gacacgcgtg agctgttcgc tttcgatcgc
     2641 gttggtcatg tctcgctccc agtcagttag ttgctgacaa agcagcagtg cctgcttatg
     2701 ctatttgatg gctggcatca tgaccagccc gcacctccgg aagcggtatc accgcggccg
     2761 gagcggaaga gcatgtacat cgtcgccgta atcgtttgcc gccaccgtgc cggacaagac
     2821 gaaatggagg gacgtatgct cgaacatctt gagcaacagc agaagctcac ccgcaatcaa
     2881 tggcgcatca tcgccaccgc caatctcggc gatatgctcg acttcttcga cttcttcctc
     2941 atcggctacg tgctcgcctt catcatcggc tcatggaaac tgaccttcgg ccagtcggcg
     3001 gtcatcctgt tgtcatcggg tctcggcgcg gtaccggggg cgttcttctg gggctggatg
     3061 gccgacaaat tcggccgccg caccatcttc atggcgaccg ccctcaatgt ctcgatcgcg
     3121 accggcatca tggcattcac accagaccag gacgctctga ttcccggctg gttcttcctg
     3181 gcattcttcc gcttcttcgt cggcttcggc aatgccggcc tgatcgcggt ggacattccg
     3241 ctggtgcagg aattcgtgcc cgcgtacaag cgcggctggg tcagcggatt gaccaccgtg
     3301 ctgctgcccg ccggcaactt gatgggagcg gtatctggcg ccttcctcgc accactcatc
     3361 ggctggcgtg gcctgttcct catcggtctc gcccccgccc tgctcgtgct gatgatccgc
     3421 tactgggttc ctgaatcacc gcactggctg ctgcgccgtg gccggatgga agaagcgcgc
     3481 aggtcactcg cctgggccct gcaggtggac ccgcagcaaa tagctctgcc caccagcgtg
     3541 cccgatgcag tgaaacccct gccctggaca gagttgttcc gctatccgcg gagcatcgcc
     3601 gcaggctgcc tgacggcgat cagtcagacc ggcggcgtcg ggctattgct ctggatcatc
     3661 gctctgttcg tgctcgtgct gcggatcacg ccagccgagg cctcttatct gatgatctat
     3721 gtatcgatcc tcggcatcct cggtcgcctg ttctgctcct acctctcgga tgcgatcggc
     3781 cgcaaggcgt cgggtgcgat catcggctat gccggcgcga tcaccatggc gctggccggc
     3841 tatatgaaca gcgtctggct gggcggcgtg tcgatgttct tcgtcctggt gatggcgcag
     3901 cggttcttcg gcgacggcag ctacgcgatc atcggtccct acattgccga ggtatggccg
     3961 gcgcgtctgc gcgccagtgg catgggcctg gcctacggcg ttggcaatat aggcaagatc
     4021 atcggtccgc tgggcctcgc gctgatcatc ggttcttcga actacgtcag cccgaaggcg
     4081 acgctcgacg cgatcgtgcc agcattcctc tacctggcgt tctggtacgc gctcgccgcc
     4141 accgcctttg cgttcctggc agtcgagaca aagggacgca cgatcgacga gatcgacgca
     4201 acgctggtga aggcgccggc ggccgaataa ggccgccccg cctatccttg ccagaccgac
     4261 acgaggtcct tcaccgtcgg ccgcgccgcc ggcgaaccgc cgccaacctg cgtgccaagg
     4321 tacaggaacc cgacaatatc gttgtcggcg tccagtccga acgcctgctt cacgcccgag
     4381 tcataagccg gcgcgccggt cttccacatc gcgccataac ccagcgcgtt cgccgccagc
     4441 atgatattct cggccgcggc ggcagcagac accaactgct ctacctcggg gatcttatgc
     4501 ccgcgctgca ctgtcgccgc cgccaccacg atcaccggcg cacgcatcgc cttgtcccgc
     4561 tcgcgttgca gcatgtcggc ggacgcgtcc ggctgctgcc ggcgcaaaca atcagccatg
     4621 atctcgccaa accgctcgcg cctttcctcc ggcaccacga tgaagcgcca cggccgaagc
     4681 cgcccgtgat ccggtgcgcg cagggcactt tggaagatgc gctccagtgc ctcctgatcc
     4741 ggcccaggcg atgtcagttt cgtcgccgat tcacgagcga ccagcagttc cattgcgtcc
     4801 atcgagcatc ctccctcagt gtgcgagcgg accgtccgcc tttttccacg cgtcgatgcc
     4861 gccgtgaata tggcacgcgc tcgcaagccc tgcatcctgc gctgcctgca cggccatcgc
     4921 cgaacgttcg ccgaacgcac agtagaacac cagccgcttg cttgtcgcgg tggccagctc
     4981 gtgcagcatg ccgccatcct ggatgttctc ctgcaggtcc ggatacggcg catgcagcga
     5041 gccaggaatc accccgtgac gctcgcgttc cgtgcgttcg cgcagatcga tcagcgcgac
     5101 gtcgcggttg ccgatcagcg ctttcgcctc gtcggccgtc attgaccaac cgcgccgcgc
     5161 aatctcctgc tgcgccagcc cctggcgcat attcgccggc accgcgacat ccatcatctt
     5221 cgggttggac aggttcaacc cgttcatcag cgccatgtac tcatcgacgg acttcacctg
     5281 aagccggggg ttgtaacgct tttcctcccc gatcgtgctg accgtatccc ccttgtaatc
     5341 atgcgccggg aacaccatcg tctcgtccgg caggcgcagc agccgattga agatcgactc
     5401 atactgcgcg cgcggatcgc cattctggaa gtcggtgcgc cctgtcccac gaatcagcag
     5461 cgtgtcgccg gtgaacacgc gatcaggcat gatgaagctg tagctgtcat cagtatggcc
     5521 aggcgtatag gcaacatcga ggctgatgcc ctcgatgccc agccggtctc cctcgccgat
     5581 gcgcatggac accacgtcca cactggattg ctcgcccatc acggtgatgc atcgcgtccg
     5641 gtcgcgcagc gcccccaggc cggtgatatg gtccgcgtgc agatgagtat cgatcgcctt
     5701 gaccagcttc aggtccagct cgcggatcag ttgcaggtag cgatccacct tctccagcac
     5761 cggatcgatg atcagcgctt cgccgccgcg gcgggaggcc agcagatagg tgtaggtgcc
     5821 ggatgtcgcg tcgaaaagct gacggaagat cacagccatc tcctgagaag ggttccagga
     5881 gaaagtttta tccaccgaat gccgtccgac tcaagaagat accacttccc acttctcgat
     5941 cgtgcgtttc tcgatcgtgc cgacgatcac cttttcggtc gcatagccca ccacggcgac
     6001 caggatcatt gccgtcatca gatcgaccgc ccgaaactga aaccgtgact ccatcagcag
     6061 gtaaccaatg ccgtcctggg acgccagcat ctcgccggcg acgatcatgc gccacgcacc
     6121 gcccaggctg atccgtaggc cggtgatgat gaacggcacg gtcgccggca acacgatcct
     6181 gaagaagaac gagacacccc gcgcaccgaa gcagcgccca gcgtccagca cggtcgggct
     6241 gatctgccgc gcaccggcct cggcgttgaa cagcaacggg aagaacaccg tagcgaacac
     6301 cacgaacacc accgccgcat cgcccagccc gaaccacaat atcgccagcg ggatccaggc
     6361 aatgcccggg atcgggtaga cgccctggat cgccggcatc atgaactcgc gcaaccagct
     6421 gatgcgccca agcgccaacc ccagcggaat ggcgagcacg gccgcggaca cgaacccggc
     6481 catggcccgc aggaaactgt gccagagatg cacatagatg ttcggcccat acagccagtg
     6541 tggatcagag acagtcagtg tcgcgaccac gttgcgcagt acggtcagcg gttctggcag
     6601 aagcagctcg aggtcgagca cctgcgacat cacctgccac gccagcaaaa tcagcacgac
     6661 gcccgtcgcg ccccgtatga agcgccgcag ccgcacgcgc cggttccgtg ccttgacgta
     6721 gcgcgcaaag ttgtccgacg ggcggctgac ccgtggctcc gcggcgattg cagcgatccg
     6781 ttcgaccgtg ttcatgactt ctgctgcacg tagtccagat caaacacgtc attcactgtc
     6841 atgttctgcg agatctcgcc acgcgccagc atcgcccgcc ccagaatgtc ccaccacgcg
     6901 ttggccgctg ccgcggtcag accaacgcga ggatcgaagc gatcgtacat cgcctcgatc
     6961 accgccggat cctgccggga ccattcggcc atgatcgcca ccatttccgc cctgggcgat
     7021 gtcgcataac tggcgcccgc atcacgcagc gcctgggtga agcgtcgcgc cacatcggca
     7081 tgcgccttga tgaactgcat gttgccgcaa acaccggtcc ccgggacgag acttgcttcc
     7141 tggccaaagt cgccgcgatt gctgctcatg aactcgaatc cggcattggc acgaagcgcg
     7201 agcgttgttg tcagcgcatc gtgcatgaat ccgtcgagct gatggctgac cagggccggc
     7261 agcatctccg ggccgccaac ggtcaggaac tgccacttga agtcgtccgt cgtcagtttc
     7321 ttcgtagcga gaaaggcccg gaacatgatc aggtggaatg aagacggatt cgacaacccg
     7381 atcttgagcg gcatctgcct cagttcgtcc agtgacttgc ccttcagatc attgcgcatc
     7441 acgaagaatg taccagcgga aatcccacct tcgatcaacg caatcggcaa cgctttgcca
     7501 gcttccagat agcgcgaatt ggcatgcgcg acctcgccgc tcgcaccgag atagaagttg
     7561 atgtcgttgc gcagcaactg ctcgatcccg gcagcggtct gacccgacgg caccggatat
     7621 tcggcgattt cgaggccatt cttcgcaaaa gcacccgtct tcaccgcgat ccagtagatc
     7681 acggtgacga tattcggctt cagcataccg accttgatct tgtcggcggc gaaggcccgc
     7741 gagcgccaca gtcccgacag aaccgccaca gcgggaagcg ccagcaggcc gcgccgcttc
     7801 attgcaggat cctcccgaat gtcgccgcgt tcaccacggc gctgcttcga tcttcacgct
     7861 atgagtcgca ttctgcatgg tcaagccatt cggcgacgag atcgcgcgcc tcgcgcggcg
     7921 ataccaccgg atgcctgatg agatagagcg tcgtgcacgc acggaaccca ctcacctcat
     7981 catcacccag gcggcatagg tcacgccaag cccgttgcac ggcgcgctgg cagctcaaag
     8041 cgccgccctc gccgcacatc gccgctagac cagcccggaa cgggcgtaga atttcactgg
     8101 attttcccag aacagcttgc gcttgcgatc ctcagacatg tgcttccagc caagcacgat
     8161 attggtcgat tcagggaatg cgctctcacc atgcggatag tccgaggcgt acatcaggac
     8221 gttttcgccc acgatgtcgg cgaccatgtt ggtgaccttc tcgccttccg acacctcgat
     8281 gctctggtag taccgcccgc tcatgacgta ctcgctggga tgatgcttca ggtcgggcgg
     8341 cagcgcggtg cggatcgtct ccatgtgctc gtccaggcgc gccatccaga acggcagcca
     8401 gccatgcccc gcctccagac aaccaaggcg cagcctcggg aaacgatcca gcagcccgct
     8461 gccgatgagc gccgccatgt tacgcatgcc gcaccacgga tgcgctgccg aacgctgcag
     8521 ccagagattg tcccacgtat cgagcccacc tggcgcataa ggcggcatca ccgtgaacgt
     8581 gtgcagcacc gctgacagat tgagctcctg cgcttccgcc cagatcggat ccagcgacgg
     8641 tagatcgatc ggtgtcccat agggtgcgta gaccagcacg ccccatgccc agcgctcgcg
     8701 tccggcccgg cgcatctcct cgacgcttcc ctggacatcg cgcgagctca ccagcacgac
     8761 accccccagc cgatccgaat agggcgcgca gtaatccttc atccagcgat ggaacgcacg
     8821 atacatgccc atctccagcg ccacgtcatc gccggcagtg aaggtgccga accagccgga
     8881 tggcagcatg aagttcacat ccgcgccctc gaaatccatg tcggcaatcc gcgcggcgga
     8941 agaccgatcg acatccggcg aaatagcacg ctcgcgcttc acgccggtga agcccgccat
     9001 atagccacct ggtgccgcct ccgtggtctc ggaatacagc cgcctttgat aacgccgctg
     9061 ccctttgttg taggaaacat ggccattctt gctgctggac ctgtactgcg accacgcttc
     9121 cagccgccgc ttgtcgtccg cgctcagata cgtctccagc acatcaacgt aaggtccgac
     9181 gtgcgcgtcc gtgtcgaaga acttgtaacc accgcgtgcc atacttggtc tccctcagga
     9241 cagtgcaccg ccggatgaac ggcggtattt ctccagcgcg gctgctgaca gatagcgttc
     9301 ctccaattcg cgctgcttat cgagcccctc accatcagca ggaacttccg gaatcgtgta
     9361 aggatgctgc ttgcggctct cgacgaacgc ttcccacgcg ccgattccct gcgcactgaa
     9421 cgcctccatg aagtccgaca tcgccgccca gatcacgtga tgcgaaaagc tcggcagcca
     9481 ggcgatcgtt gcgcctagcg ccagatgttc gtccagcgac agataccgcg gcagcttgcc
     9541 cttcatgaac gagaacggac acgtcactgc cgcccgcgcc tgcctgatct cgtccaccga
     9601 atgcggcgac tcaagctgga cccagtccac tccagcctcg cggtgatacg ccgcgaggcg
     9661 ccgcagcgtt tcctccagtc ccccatttgc cgcatcgcgc gcatagcatt gcgccatcac
     9721 cacgaaatcg gggtccagtt cgttcttcat gtccaccgcg gcgcggtagc ggacgatcgc
     9781 ctgctccagc ggcacgacct ccagccccgc gctctgagtc cgccgcttcc cctcgatcgg
     9841 ctgatcatcg atgcgaatgc cggcgatacc cgcgtggata cactcccgta ccagccgccg
     9901 caccgccatg atgccgccat gcccggtatc gccgtcgatg atcaccggcg tggacacgct
     9961 gttggcaatc cattccgcaa tctttacgca ttcggtcatc gtcgcggtgc cgacatcggc
    10021 cattccggtg tagccgccga ccacggcgct ggtgccgacg aacagcgcct cgcacccacc
    10081 tcgctccatg acacgcgcca atgctgccgt tggcgggtgc atcaccgaca gcacctgccc
    10141 cttgcgatga atgagctgct tcaggcgcgt ggacgcgcgc gcgccgtcac tcggtggcaa
    10201 cgccatgtcg ctgcatctcc tgcatgatgg tggacttcac cgcgagaaac tcggcggaat
    10261 gccgatcacg cggacgcggc agatcgttgc gaatgtcggc ggccagccgc ccaggcgaag
    10321 gccgcaggat cacgatgcga tctgcgagat agaccgcctc ttccagatcg tgcgtgacgt
    10381 agagcacggt tctctgctcg gtcagccagg tattcaccag gtggtcctgc agtgtcatgc
    10441 ggttgatcgc atcgatcgcc gagaacggtt catccatcag caacaacgcc gcatcggtca
    10501 gcgcccaggc acgcgccacc gcgacgcgat gcttcatccc gccggagagc tggaacggat
    10561 atagcgaggc ctgccgtgcc agtcctacga tctccagcac gtgcatcgcc cgttcgcgcg
    10621 ccgcggtctt cttccagccg cccatcagca acccgaattc gacgttcttg atcaccgtca
    10681 gccaggcgaa caatgccggg tcctggaaca ccacgccgcg gtccagaccc gggcccttga
    10741 tcgggttgcc attcagcagg acccggccat tggttgggtg catcagcccc gcgacgatct
    10801 tcaggaatgt gctcttgccg cagccactcg gcccaaggat gcacaggaac tcattgcgcc
    10861 ggatcgtcag cgtcaggtga tcgagcgcgt gattccaacc gagctgcccc tcgtagaagt
    10921 gatcgacatc ggtcgcctcg atcaccggcg gtgcgatcga cggttcagcg atgaccgtgg
    10981 ccgcgcccgt catgcgcgaa caccgttcat aggccgcatc actctggacg tcctctctgg
    11041 tttcatatat tgaaactcag ttttaatccg atgaacgcat gctaccggcg gcggccccgc
    11101 tgtcaagcag tgcgcgggaa aggctgccgt gatgggtatc gccgccagcg gttccgagcg
    11161 ctacagcgtc tcgacggtgg agcgcgcgct ggaggtcttg cgcgccttca cctatcgcca
    11221 accggagctg tcgctgaccg atatcgccgc ggccacgggt ctgcacaagg caaccgtgtt
    11281 tcgtctgctc gccacgctct gcaacggcgg gatgatgttg aagaaccccc gcaccggcct
    11341 gtaccggctc ggctacggca tcatcgccat gtcagagatc gcgaagacca ccaccggctt
    11401 cgtttcgcaa gcgcgtccct tcatgcgaca gatccgcgat gagctgaacg agactgtcta
    11461 catcgccgtc cgcgtcggcg atgacaggat caacctggat cagcttgagg gcatccgcga
    11521 cttccgccgc gtggtcacgc tcggaaaacc aagcccgctt tacgttggca gcaccagcca
    11581 cgtgatcctt gccgcgctgc cggatgacga gatcgcggcc tatattgccc ggaccgagtt
    11641 cgtacctcct ttccctggcg ccaggataga tgcgcagacg ctgcgccgcg ccatcaccga
    11701 cacgcggcgc aacggtttcg ccgagacgca taacaaacgt atcgacgaag gtgcctcgat
    11761 ctcgtcgctg gtccgcgggg caggcaacga gatcatgggc gcgctcaccg tttcgatccc
    11821 aatcggtcgc tacaccaaca ccgttcgcac tcgcacgatc gagtttgtaa ccggcgcggc
    11881 cagcgccctc tcagggcagc tcggtgccag cccctgaagc ggctaacctc tgacgcggcg
    11941 catggcggca accgtgccga tcagacccat cccaagcagc agcaacgaac cgggctcggg
    12001 taccgctgtc gtcttgatcg aaagtccact ctgacccggt gccgatggat cggtcgagac
    12061 gctgttcgtc cagttgatcg atgcgttcgc cacgttggca ttgaactccc aggcataggc
    12121 gttgatgcca aggtggtgtc atagatgccg gaaacgtcga actagacttt ggcgccaccg
    12181 ctatcaatgc tggaacggat gttgtcgttg ccgacatcga gatcaacgaa catcaggtag
    12241 gacgcggtcg atggatcatt gatgttctga cccgagtaga acggcaggac aaaaccgttg
    12301 cccgggcccg gtttcgcggt ctcgggccca tagggaaaat ccgacttcgt gaaggtcttg
    12361 ttgacggccc caaccttata gatcggattg accacgccgg cgttcgatgg tgtccactga
    12421 taaccgctcg acttgatcgt cagacagaaa ttgttggtga ccggtccctg caccgagaac
    12481 gccaggatta tatcgtcgtt gtacccacga ccaccggtcg tgctgaccca gaacgtgccg
    12541 gtagccgacg aagtggcgac gtcctcgcgg tgacctggcc agatgtgccg cttgcggagc
    12601 tgtcagtcgt gatgtggagc tgattcaatc caccgccagg ggcatcgagg aattagctat
    12661 cgttcggacc agtgccatat agcgcggcag catcattgga catctgaatg tagatgtccc
    12721 ggttggctgg caacggcgca gcctcagatg cgggaatatc cgacagaatc agcagcagca
    12781 aagcaccgga tgacgtatac cggattacgt tcgacgtggc tccccctcgt gagcgctata
    12841 tccagtcagg aattctgcat atgccagatc gcggacaatg tcttccacaa gcgtagtata
    12901 tcatcgcgga ttaggaaagg tggctatgcc ccgatggaca tggtgatcat gttgccgcag
    12961 ggcgacagcg tcgccgctag gacggcattg cctcgtatac cgtcaccccg tcggaatgat
    13021 cgcccaggca ggacggccac gcgcttagat cgcgatactc ggcatgaaca aggttggatc
    13081 gcccagcgtg ccggcataga tcgtgtgtga cgtgtccttc ccaaccacgc cgccgaacac
    13141 caggctgcca cagaccgcgc agaagttgcg caccgcatca gtggcgctcg cggaccggct
    13201 ggtgaagctg cgtgtcgcac ctgtgaagtg gaccgcggtg ctcgcgatgc cgataaacgg
    13261 caggaagccc gagccgcacg ccttgcggca gtccgcacaa tagcagtgtc ccatcaactg
    13321 cggtgtgcca tgcaccacgt agcgaagcgc g
//
LOCUS       DATGEM010000230         6256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_481834, whole genome shotgun sequence.
ACCESSION   DATGEM010000230 DATGEM010000000
VERSION     DATGEM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481834"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VKQ27_07235"
     CDS             <1..352
                     /locus_tag="VKQ27_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY88762.1"
                     /translation="ASFFLPRLVGEARARALAMLGDAIDAERAEAWGMIWKVVDDVAL
                     QTEAEALTANLARQPTASLAAMKRMFAAATANSLEAQLDLERDEQAAAARRADYAEGV
                     RAFVEKRAPAFRGR"
     gene            470..889
                     /gene="paaI"
                     /locus_tag="VKQ27_07240"
     CDS             470..889
                     /gene="paaI"
                     /locus_tag="VKQ27_07240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HLY88763.1"
                     /translation="MTSATDVVRARAEAMWAEDRATNGLGMRLDDVAPGRARLSMMIT
                     EVMANGHGICHGGFIFALADSAMAFAANPRGEPAVAQHASITFVRPGRIGEVLVAEAV
                     ERMHAGRSGIYDVRVSTEGGELVAEFRGHTRTIRAEK"
     gene            891..2180
                     /gene="paaK"
                     /locus_tag="VKQ27_07245"
     CDS             891..2180
                     /gene="paaK"
                     /locus_tag="VKQ27_07245"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HLY88764.1"
                     /translation="MAGLDPIETASRDEIAALQRERLAWALRHAYTNVAHYRAAFDAA
                     GVHPDDFRSLEDLAKFPFTTKQDLRANYPFGMFAVPREKLVRVHASSGTTGKPTVVGY
                     TAKDIETWAHVVARSIYAAGGRAGMIVHVAYGYGLFTGGLGAHYGAEKLGCTVVPVSG
                     GMTERQVQLITDFRPDIIMVTPSYMLAILDEFRRAGLDPRASSLNIGIFGAEPWTNAM
                     RQEIEAGFAMDAVDIYGLSEVMGPGVANECVETKDGLHIWEDHFYPEVIDPETGAVLP
                     DGSSGELVFTTLTKEGMPVVRYRTRDLTRLLPGTARTMRRMEKVTGRSDDMVIVRGVN
                     MFPTQVEEQLLKSDVLTGHYQIVLTRKGRMDEVAVHVEARPEYFDPVLLEAEAKVVEA
                     RIKDTIGLTTRVVVEAPGSVERSIGKARRVVDQRPKE"
     gene            complement(2192..3397)
                     /locus_tag="VKQ27_07250"
     CDS             complement(2192..3397)
                     /locus_tag="VKQ27_07250"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88765.1"
                     /translation="MADLTTRCCVVGGGPAGMMLGYLLARSGIDVTVLEKHADFLRDF
                     RGDTVHPSTMQVMHELGLLDAFLTRPHSEVRNLAAQIGDELIHLADFSHLPTHAKFIA
                     FMPQWDFLDFLADQARHLPTFHLLRRTEATGLVRNAGHVVGITATGPDGPLTIHAELT
                     VACDGRHSTLREAAGLPVTEIGAPMDVLWLRLSKHAADPGQSFGHIDKGRFFIMLDRN
                     DYWQCAFVIPKGGIDDVHAHGLEAFRAEIVQLSPFLSDRVQELATWDDVKLLTVAVNR
                     LETWYQQGLLLIGDAAHAMSPVGGVGINLAIQDAVATANVLVPRMKAGTPTLEDLRAV
                     QRRREWPTKMTQRMQVVIQNRVITNVLGSTQTPKPPALLRLVGHTSLLQRIPARVIGM
                     GFRPEHIRH"
     gene            complement(3398..4657)
                     /locus_tag="VKQ27_07255"
     CDS             complement(3398..4657)
                     /locus_tag="VKQ27_07255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88766.1"
                     /translation="MKFMWFHLMPYTELPEDFRDSHPSVWVDIHSSLFDPRRAHHMYN
                     DFMDELEFAAQCGFDAICVNEHHSNGYGLMPSPNLIASSLARRTTDTAICVMGNSLAL
                     YSPPTRVAEEFAMIDVISGGRLIAGFPVGTPMDTCYAYGQNPSQLRERYHEAHDLIMR
                     AWTETDTFAFNGRFNQQRYVNIWPRPIQRPHPPIWIPGGGSIETWQWCAEQDYVYSYL
                     SYYGHKVGQATMDGFWKEMSRLGKDRNPYRAGFAQTVAVAETREQAMDLYTEAAEYFF
                     GRCLHIDQRFVAPPGYTTEATTRVGLQSQVAKAAAYSDKFKLLPTKMPDLVQNGYIVL
                     GSPDDVIQQIEELANNLNVGNLMLLLQFGNMGKDLTKYNTKLFAERVMPKLRQKFGEW
                     DHRWWPQPMDTAQRAELSAYEPRQAAE"
     gene            complement(4981..5787)
                     /locus_tag="VKQ27_07260"
     CDS             complement(4981..5787)
                     /locus_tag="VKQ27_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88767.1"
                     /translation="MVTAVPETSSAASPPDPAQDHPAPTLPNNIEVFLHAVCILLAYG
                     RHMIETVCQRAAAPDFNAIAANFGTANLSTIIAHLNRGILRAFALQRVLLARAATGRD
                     VGIIKRHIPTIEEQLAAMDAPPEQPATRLTTRKYAPRPSRPAGWDDPELFMPTLEDLE
                     REARRCPVGRTMLKICLDLAVVPGFCHTQFWNELFELITYFGSNVSKIMEQKSRRQRA
                     FANEQDRNPKATWDWMNLSRDAPRRILGFFIGEPPINPLDPAAAIATAPP"
     gene            complement(5867..>6256)
                     /locus_tag="VKQ27_07265"
     CDS             complement(5867..>6256)
                     /locus_tag="VKQ27_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY88768.1"
                     /translation="SVGPAIVMPMIIGYKKARELLYFGDQIDARTALDLGMINRIVPL
                     AELRDASLAYAKRLSLISPEALYATKRAVNRVADASGFRTALYAGLDVVGPLYATNTE
                     YGARFREIAQTEGVPAAVRWRSAQFAK"
BASE COUNT         1031 a         1666 c         2429 g         1130 t
ORIGIN      
        1 ggccagcttt tttctgcctc gactggtggg cgaggcgcgg gcgcgggcac tggcgatgct
       61 gggcgacgcc atcgatgcgg agcgcgccga ggcctggggc atgatctgga aggtggtgga
      121 tgatgtggcg ttgcagaccg aggcggaggc gctgaccgcg aacctggcga ggcagccgac
      181 ggcgtcgctc gcggcgatga agcggatgtt cgccgcagcg acggcgaatt cgctggaggc
      241 gcagctggac ctggagcggg acgagcaggc ggcggcggca cggcgggccg actacgccga
      301 gggcgtgcgg gcgtttgtgg agaagcgcgc tccggcattc cgggggcggt gatgatgacg
      361 cgatcagctt tgtggtggcc ctgggcccgg gttggtggcg gttgaagcga cctattgacg
      421 atttccagcg gggaaaggaa ggcgtggata ctcgcgccgg ggcgcgagca tgacgtcggc
      481 gactgacgtg gtgcgtgcgc gggcggaggc gatgtgggca gaggatcgcg ccaccaatgg
      541 cctgggcatg cggcttgatg atgtcgcgcc gggccgcgcg cggctgtcaa tgatgatcac
      601 ggaggtgatg gcgaacgggc atggcatctg ccatggcggg ttcatcttcg cgctggctga
      661 ctcggcgatg gcgttcgcag ccaatccgcg gggtgagccg gcggtagcac agcacgcttc
      721 gatcaccttc gtgcggccag ggcgcattgg cgaagtgctg gtggccgagg cggtggaacg
      781 gatgcatgcg gggcgatccg gcatctacga cgtgcgggta tcgaccgagg gcggggagct
      841 ggtggccgag ttccgcgggc acacgcggac gatcagggcg gagaagtagg atggcggggc
      901 tcgatccgat cgagacggca tcgcgcgacg agatcgcggc gctgcaacgc gagcggctgg
      961 cgtgggcgtt gcggcatgcc tatacaaatg tggcacatta tcgcgcggca ttcgatgcgg
     1021 ccggcgtgca tccggatgat ttccgctcgc tcgaggacct ggcgaagttc ccgttcacca
     1081 ccaagcagga cctacgcgcg aattatccgt tcgggatgtt cgcggtgccg cgcgaaaagc
     1141 tggtgcgggt gcacgcgtcg tcgggcacga cggggaagcc gacggtcgtg ggttataccg
     1201 cgaaggatat cgaaacgtgg gcgcatgtgg tggcgcgctc gatctacgcg gccggcgggc
     1261 gggcggggat gatcgtgcac gtcgcctacg ggtatgggct gttcaccggg gggctgggcg
     1321 cgcattacgg ggcggagaag ctgggctgca ccgtggtgcc ggtctcgggc ggcatgaccg
     1381 agcggcaggt gcaactgatc accgacttcc gtcctgacat catcatggtg acgccgagct
     1441 acatgctggc gatcctggat gagttccgcc gcgccgggct cgatccgcgc gcgagttcgc
     1501 tgaacatcgg tatcttcggg gcggagccgt ggaccaatgc gatgcggcag gagatcgagg
     1561 ccgggtttgc gatggatgcg gtggatatct acgggctgtc ggaggtgatg ggacccggcg
     1621 tcgccaacga atgcgtcgag accaaggacg ggctgcatat ctgggaggac catttctatc
     1681 ccgaggtgat cgatccggag accggcgctg tgctgccgga cggatcgagc ggcgagctgg
     1741 tgttcacgac gctgaccaag gaaggcatgc cggtggtgcg ctatcgcacg cgcgacctga
     1801 cgcggctgct gcctgggaca gcgcgcacca tgcggcggat ggagaaggtc acggggcgct
     1861 ccgacgacat ggtgatcgtg cgcggggtca acatgtttcc gactcaggtg gaagagcagt
     1921 tgctgaagag cgacgtgctg accgggcact accagatcgt gctgacgcgc aaggggcgga
     1981 tggacgaggt ggcggtgcat gtagaggcgc ggccggagta tttcgacccg gtactgcttg
     2041 aggcggaagc gaaggtcgtg gaggcgcgga tcaaggatac gatcggtctg acgacgcggg
     2101 tcgttgtgga ggcgcctggg tcggtcgagc gttctattgg caaggcgcgg cgggtggtgg
     2161 atcagcggcc gaaggagtag gacggggcgc cttaatgccg gatgtgctcc ggacggaagc
     2221 ccatgccgat cacgcgtgcg gggatgcgtt gcagcaggga agtgtggccg acgagccgca
     2281 gcaatgcggg tggctttggt gtctgggtgc tgcccagcac gttggtgatc acccggttct
     2341 ggatcaccac ctgcatgcgc tgcgtcatct tcgtcggcca ctcgcggcgg cgctgcaccg
     2401 cgcgcagatc ctccagtgtc ggcgttccgg ccttcatgcg cggcaccagg acgttcgcag
     2461 tggcgaccgc atcctggatc gcgagattga tgccgacgcc gcccaccggc gacatcgcat
     2521 gcgccgcgtc accaatcagc agcagtccct gctgatacca ggtctccagg cgattgaccg
     2581 cgacggtcag cagtttcacg tcatcccagg tggcgagttc ctgcacccga tcgctcagga
     2641 acggtgagag ctgcacgatc tcggcgcgga acgcttccag gccgtgcgcg tgcacgtcat
     2701 cgatgccgcc cttcgggatg acgaaggcac actgccagta atcgttgcga tccagcatga
     2761 tgaagaagcg gcctttgtcg atgtggccga acgactggcc aggatcggcg gcatgcttgg
     2821 acagacggag ccacagcacg tccatcggcg cgccgatttc cgtcacgggg aggccggccg
     2881 cttcgcgcag ggtggagtgg cggccatcgc aggcgacggt gagttcggcg tggatggtga
     2941 gcgggccatc cgggccggtg gcggtgatac cgacgacatg accggcattg cgaacaagac
     3001 cggtggcctc ggtccggcgc agcagatgga acgtgggaag gtgccgcgcc tggtcggcaa
     3061 ggaagtcgag gaagtcccat tgcggcatga acgcgatgaa cttcgcgtgc gtcggcaggt
     3121 gggaaaaatc ggcaaggtgg atcagttcgt caccaatctg cgcggcgaga ttacggactt
     3181 cgctgtgcgg ccgggtgagg aaggcgtcga gcaggccaag ctcgtgcatg acctgcatcg
     3241 tggagggatg caccgtgtcg ccgcgaaagt cgcgcaggaa atcggcgtgc ttttccagca
     3301 cggtcacatc gatgccggag cgcgccagga ggtagccgag catcatgccg gcgggaccgc
     3361 caccgacgac gcagcagcgg gtggtcaggt cggccattta ttcggccgcc tggcgcggtt
     3421 cgtaggcaga cagttcggcg cgttgcgcgg tgtccatcgg ctgtggccac cagcggtggt
     3481 cccattcgcc gaatttctgg cgcagcttcg gcatcacgcg ttcggcgaac agctttgtgt
     3541 tgtacttggt gaggtccttg cccatgttgc cgaattggag aagcagcatc aggttgccaa
     3601 cgttgaggtt gttggcgagt tcctcgatct gctggatcac gtcgtcggga gaaccgagca
     3661 cgatgtagcc gttctgcacc aggtcaggca tcttcgtcgg cagcagcttg aacttgtcgg
     3721 aataggcggc agccttggca acctggctct gcaggccgac gcgggttgta gcttcggttg
     3781 tatagccagg gggcgcgacg aagcgctggt cgatgtgcag gcagcggccg aagaagtatt
     3841 cggcggcctc ggtgtagagg tccattgcct gctcgcgggt ttcggccacc gcgacggtct
     3901 gggcgaagcc ggcgcgatac ggattgcggt ccttgccgag gcgcgacatc tccttccaga
     3961 agccgtccat ggtggcctgg ccgaccttgt gaccgtagta ggagagatac gagtagacgt
     4021 agtcttgctc ggcgcaccat tgccaggttt cgatggagcc gccgccgggg atccagatcg
     4081 gcggatgcgg gcgctggatc gggcgtggcc agatgttgac gtagcgctgc tggttgaagc
     4141 ggccgttgaa ggcgaatgtg tcggtctcgg tccaggcgcg catgatcagg tcgtgggctt
     4201 cgtgatagcg ttcgcgaagc tgcgacgggt tctggccgta agcgtagcag gtatccatcg
     4261 gtgtgccgac ggggaagccg gcgatcaggc ggccgccgga gatgacgtcg atcatcgcga
     4321 attcttcggc cacgcgcgtc ggcgggctgt acagcgcgag cgaattcccc atgacgcaga
     4381 tcgctgtgtc tgtcgtgcgg cgggcgagcg aggatgcgat caggttgggc gacggcatca
     4441 ggccgtagcc gttggagtgg tgctcgttga cgcagatggc gtcgaagccg cattgggcgg
     4501 cgaattcgag ctcgtccatg aaatcgttgt acatgtggtg ggcgcggcgg gggtcgaaga
     4561 gagacgagtg gatgtcgacc cagaccgagg ggtgggagtc gcggaagtcc tcggggagtt
     4621 ccgtgtatgg catcagatgg aaccacatga atttcatggc gtgcctcccg ggcgcttgga
     4681 cgggagtaga tcatttgttg gggtggtttg ggaaccgagc agtctcagcg gcgtctcgtt
     4741 tcgctgtggg tgagacgggt ggaggcggtg cggcgggggg ctcagctatt tcacacatag
     4801 ggcgtggagg gaggcaccaa gctcgcagcg gcgtttgtgg cgcgatccgc ttggcgtttc
     4861 tgtgtgcgtt ctgaggctct gcgcctcggt tgtggatacg tcgatttcgg gcagcgcggg
     4921 cgccgccagg gaggcgggga ctgttgccga tggttactga aagggcatga ccgggcgcgg
     4981 tcagggcggc gcggtggcga tggcggccgc ggggtcgagc gggttgattg gcggctcgcc
     5041 gatgaagaag ccgaggatcc ggcgaggcgc gtctcgactg aggttcatcc agtcccaggt
     5101 ggctttggga ttgcggtcct gttcattggc aaatgccctc tgtcggcggg acttttgctc
     5161 catgatcttg ctgacgttgc tgccgaagta ggtgatgagc tcgaacagct cgttccagaa
     5221 ttgggtgtgg cagaagccgg ggacgactgc gagatcgagg cagatcttga gcatggtgcg
     5281 gccgacaggg cagcggcgcg cctcgcgttc caggtcctcc aatgtgggca tgaagagttc
     5341 gggatcgtcc cagccggcgg ggcgggaggg gcgtggtgcg tacttgcgtg tggtgaggcg
     5401 ggtggcgggc tgctcgggcg gcgcgtccat cgctgcgagc tgttcctcta tcgtggggat
     5461 gtggcgcttg atgatgccga cgtcgcggcc ggtggcggcg cgtgcgagca ggacacgctg
     5521 cagggcgaag gcgcgcagga tgccgcggtt gaggtgggcg atgatggtgg agaggttggc
     5581 agtgccgaag ttggcggcga tggcgttgaa gtcgggcgcg gcggcacgct ggcagacggt
     5641 ctcgatcatg tggcggccat aggcgagcag gatgcagacg gcgtggagga agacctcgat
     5701 gttgttgggc agagtggggg cggggtgatc ctgtgccgga tcgggcgggg aggcggcgga
     5761 cgacgtttcg ggcacggcgg tgaccatgcc ggggttttag caaaaacgca gagagcgagg
     5821 ccaggaaaat tttccgacgt gtgggcagat agtcaggcca ttgggcttac tttgcgaatt
     5881 gagcggagcg ccagcggacc gcggcgggta cgccttcggt ctgagcgatc tcgcgaaacc
     5941 gcgcgccgta ttcggtgttg gtggcataga gcgggccgac gacgtcgagc ccggcgtaga
     6001 gcgcggtgcg gaagccagac gcgtcggcca cgcggttgac ggcgcgcttc gtcgcataca
     6061 gcgcttccgg cgagatcagc gacaggcgtt tggcgtaggc gaggcttgcg tcgcgcagtt
     6121 ctgcgagagg aacgatgcgg ttgatcatgc cgaggtcgag cgcggtgcgg gcgtcgatct
     6181 ggtcgccgaa atagagcagc tctcgcgcct tcttgtagcc gatgatcatc ggcatgacga
     6241 tggcggggcc caccga
//
LOCUS       DATGEM010000231        13683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_822131, whole genome shotgun sequence.
ACCESSION   DATGEM010000231 DATGEM010000000
VERSION     DATGEM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822131"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..956
                     /locus_tag="VKQ27_07270"
     CDS             90..956
                     /locus_tag="VKQ27_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLY88769.1"
                     /translation="MAIRKLLLPLTGTAAGEAALTTSLMIARLWNAHVTALHVRVDSR
                     DVAPLAGEGLSGAMIEEMMSATEKESSDRAHAVRSMFDRFVAQHDVILSEAHPGTDAA
                     TASFASVTGREEDLVAQQARLADLTVVPHPDAGEDVSSSDALHAVLFDSGRPVLISPR
                     TAPTTIGSRLCLGWNGTAESASAVYAALPWLKRAEAVRILSAEGYQRRGPAAPELAAY
                     LALHGVHADIHMFQSVGNSVGAGLLAAARDFGCDLLAMGAYSHSRLRQLILGGVTRHV
                     LENAGLPVMMNR"
     gene            958..1389
                     /locus_tag="VKQ27_07275"
     CDS             958..1389
                     /locus_tag="VKQ27_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY88770.1"
                     /translation="MSEALDAYVKLLRANRAVLARVEPRLVASGLTTTQFGVLEAILH
                     KGPLSQRDLSLKVLTSPGNMTDLIDKLEARGLVRRVRQKADRRAVRVELTVAGAALIE
                     PLFAAHAGDIDAAMAGLDGDELRQLGTLLRKLGLAAGGVED"
     gene            1457..2155
                     /locus_tag="VKQ27_07280"
     CDS             1457..2155
                     /locus_tag="VKQ27_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLY88771.1"
                     /translation="MIEVRPFGELGAFRNEWLNAKHHFSFGGYHDPKRMGFGRLRVWN
                     DDEVAAGTGFDPHPHREMEIVTYIRQGAITHRDNLGNEGRTEAGDVQVMHAGTGIVHA
                     EYNREKTPTRLFQIWIQPDRHGAKPGWSTRQFPREGGLSVLASGRAADAESGALSLNA
                     DAAVLAGTLAAGETARVTLEPGRNVYLVPATGTVEVNGVKVSTRDGAAISGEAELTIA
                     ATEPSEVVLVDVAS"
     gene            2225..3739
                     /locus_tag="VKQ27_07285"
     CDS             2225..3739
                     /locus_tag="VKQ27_07285"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HLY88772.1"
                     /translation="MRFPLRGLVLASLLVPGVASAQNVTQEQAAALRQQLTDTLSELL
                     GPAVKLQDLPLKITANGDHYDMSLPIPGLEVTTGDPAVTAELKPLDGGRWSLEKARFP
                     STGNFTFTLPDTSAGPGGPMKGTYSIGKQDLQSTIDPSFASPSTSHSAVEALAITMDG
                     PSKHQEEHIDRMVGEAVLTPTKDGRLDLTSDATAEGLKSAALEDNKPAIAFGAKRVHA
                     TTHAKGINREHVAEFWGAFVKFIALVPKPPEKKEGETELPPLAKAQLRLMIAAMQDTV
                     SSISMQETVDDMQIEIADKGGATIKQLQFGFGGEAPDGSLHTWLDLGFEGVESPTMPP
                     KLATYLPKHFEIKPTLSGVPVAVLQKLAMDASDDDPAKHPVGPDLAAIFANGGAVIGL
                     ETLSFDLGPATLRGTGKVTVTSPSSWVGDAHVVATGFDELATQARENPDLQQALPVLV
                     MMRGLAKPDGDKLVWDIASEGPKLTVNGIDMSALTGGGGDKGKPPHPPHPGAKP"
     gene            complement(3718..4374)
                     /locus_tag="VKQ27_07290"
     CDS             complement(3718..4374)
                     /locus_tag="VKQ27_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLY88773.1"
                     /translation="MSPLVRDDAAFILGQTRAATAPLVPEITLQLATEITPIWQATET
                     WCHGNNAEPPFWAFAWPGGQALARYILDHPATVAGRRILDFAAGCGIAAIAAMRAGAA
                     SAEAAEIDPLARAALSLNAAANGIALTLPTSDVVGGRSRWDLILCGDVCYEAPMTSHI
                     LPWLRALATEAEVWIADPGRAYLPRDGLTPFATYAVPTSLELEDRTEREVTLYGLAPG
                     "
     gene            complement(4450..5253)
                     /locus_tag="VKQ27_07295"
     CDS             complement(4450..5253)
                     /locus_tag="VKQ27_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88774.1"
                     /translation="MNLAADQRPVASPPDPPQDNRDSTVPSNITIFLHAVGTLLTYGR
                     HLLDTVRQRATAPNFHAIAANFGTARLATILAHLHRGILRAEALERVLLARVAAGEDV
                     DFVLRRTRASAAATAPADTQPSQQVAPIPTRQRAPRPSRPAGWDDPELFMPTLEDLEC
                     LARRRPLGRTIYDICLDLAVVPGFCHSAFWNELFELMTLFGGSIDKLMREKTRRRQAF
                     SNEQDRNPKATWDWVNMSREAMRQALGFFIGEPPVNPLDPAAAVATAPP"
     gene            complement(5411..5554)
                     /locus_tag="VKQ27_07300"
     CDS             complement(5411..5554)
                     /locus_tag="VKQ27_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88775.1"
                     /translation="MGDSAKLPNSGPGSYRFVEHRRYLEALLEGLGVRERVTFVIHGP
                     CRG"
     gene            complement(5560..5745)
                     /locus_tag="VKQ27_07305"
     CDS             complement(5560..5745)
                     /locus_tag="VKQ27_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88776.1"
                     /translation="MISAAFPYQKQRRRVLDSEMAYVEVGQGDPTVLLHGNPTSSYLW
                     RNVLPHLQRLGVASRQT"
     gene            complement(5873..6610)
                     /locus_tag="VKQ27_07310"
     CDS             complement(5873..6610)
                     /locus_tag="VKQ27_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLY88777.1"
                     /translation="MSRSPLYERVESVLAGDIADGNLSPETQLPSEDGLIERFKVSRT
                     TVRKAIQNLVERGLLEIRRGKGTFVTQPKITQELTELTGFVEDMQALGRTPTARLLDK
                     RIVSADEPVAQHLGLAPGTPVVRLRRVRLADGVPMSFDETYLPRVLGEKIAANDLAAE
                     PVFALLENKYETPLLEAEYKLEAAAADSVAAEALEVPVGSPIFLIERTSYSAGNQPVD
                     YEKLHYRGDLIRFVTRLARRPKASIPK"
     gene            6727..7248
                     /locus_tag="VKQ27_07315"
     CDS             6727..7248
                     /locus_tag="VKQ27_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLY88778.1"
                     /translation="MRRIHGRSTEWGDLLTGRIGSSAFAYWAPFPLRLIVGYGFMEHG
                     FAKLARGPEAFPLILQALHVPAPHFMGWLTIVVEIFGGLAVLVGALVPLASIPMAAVL
                     LVAMFTVHLQYGFSSIKLQAVTAAGAQFGQPGFETDLLYLACLAALVLGGAGPLAIDG
                     LMTRRRDRHRLLG"
     gene            complement(7306..8226)
                     /gene="ubiA"
                     /locus_tag="VKQ27_07320"
     CDS             complement(7306..8226)
                     /gene="ubiA"
                     /locus_tag="VKQ27_07320"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HLY88779.1"
                     /translation="MMPRTVAAMAHTDIVATGWVARLPRSWLPYLLLARVDRPIGAWL
                     LFLPGLWGILLAGPAPAETIRLILLFAIGSMVMRSAGCVVNDLWDRDLDCQVARTAGR
                     PLASGMLRPRHALVFLVILLLAGLLILLQLNRLAQILGVASLLLVALYPLAKRVTWWP
                     QLMMGFTFGFGAPLGYAAGAGHLGAAWATLYGAAILWDLGFDTIYAHQDREDDALVGI
                     GSTARLFGEHTAPFLAACYAGTIILLGIAGWLTGLGPWFYPALLLPAALLARQVMQLD
                     INDPALCLRLFRANREAGLAAGLAILIGWA"
     gene            8225..8932
                     /locus_tag="VKQ27_07325"
     CDS             8225..8932
                     /locus_tag="VKQ27_07325"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HLY88780.1"
                     /translation="MTGSIRLFVDAPLAVGETIAGSAGQAHYLGTVMRRGVGDAVLLF
                     NGCDGEFAARIALLRRDRVELHIERLLRAQSQEPDLWLLFALLKRDATDLVMQKATEL
                     GVSALHPVITERCNTHRMNAGRLRAIAVEAAEQCERLTVPVLHEPRPLQEVLADWSPD
                     RRLVAALERSDAPRIAAVRGQTALLVGPEGGFSPAELEALRVHPFVTGVSLGPRVLRA
                     DTACIAGLALLQGGDCG"
     gene            8968..10347
                     /locus_tag="VKQ27_07330"
     CDS             8968..10347
                     /locus_tag="VKQ27_07330"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HLY88781.1"
                     /translation="MSNPGDADATPITSLKQLADYLAAGCKPAEQFRLGTEHEKFGFR
                     LADLRTPPYEPADGQPGSIRGLLEGLQGFGGTPILDRGAIIGLKQGDAAISLEPAGQL
                     ELSGAPLETLHQTAAELEAHYDQVRAVSNELAIGFLPLGFHPLAKRADMPWMPKGRYA
                     IMRRYMPEVGTLGLDMMTRTCTVQVNLDYASEQDMVRKLRVSLLLQPLATALFANSPF
                     TEGRPNGFLSYRAHVWTDTDNQRSGIPRVMFDPGFGFERYVEWLVDSVPMYFVYRDGA
                     YIDVAGASFRGYMNGQLHNVAGAVATVGDFADHVTTVFTDVRIKRFLEMRGADAGRAD
                     MMVAQAAFWVGLIYDDAALASAEALLRDATWEEAVALRAAVPREGLGASWRNGVLRDL
                     AQDVVAIARDGLRSRGRLDPSGRDEAMYLDPLMAIAAGEPTQAEHWLARFHQAWQGDV
                     RRIYSEGAI"
     gene            10492..12558
                     /locus_tag="VKQ27_07335"
     CDS             10492..12558
                     /locus_tag="VKQ27_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HLY88782.1"
                     /translation="MRLIGQMEAWTLTSENVLPAGRKTRALLAVIALSSPRPALRGRL
                     AELLWSRRPEEQARASLRQEIHRLLESLAPAGTEILTVTRDHLSLRPGLVWIDVEEVM
                     RATTDQPASLSLLDGDLLEDLDGIDPTFDAWLTTERERLRDRARNVAEALLREQVEPE
                     AAIPAAQRLLQIDRAHEGAWRALMRAHAARGERGMAIQAYDRCRAVLADLLDAAPSAE
                     TQKLLAEIRGPSGSRMPLRPPAPPPLPAPQMAAVVEEPEEPPAVLEPRAQEIRPTRGG
                     AHVGVMPLQLVGTSDEEAHLAPGLAEEITTALARFRWIFVVASNSLARFAADTRDETA
                     IRRTFGIDFLLDGSIQKVRNRLRITVRLLDLRAGNQVVWARRFDRQTNDLLSLQDEIA
                     SEVVAQIDPEILLIEAKRSASQPPVDATAYDLVLRAIPLIGRMERSRYIMAGEYLVNA
                     IALEPDYAAAHAWYAYWHIFLVGQGWAEDPHAMMEEAGELAERAVVLDPFDARALTIA
                     GHVRAYLHRRLHEAAALHERALSLNPNLAMAWALSAITYAYMGEAEEAERRNNRYKKL
                     SPLDPHAFFFDAFFILIHLMKRDYETAVAVGRAVSEMNPSFSATYKPYLAALGHLGRT
                     QEAAVVRRRLLAVEPDFTIERFVATSPLEREADRDHYAHGLRLAGVPERAAEVAEQNL
                     QGQEAH"
     gene            complement(12737..>13683)
                     /locus_tag="VKQ27_07340"
     CDS             complement(12737..>13683)
                     /locus_tag="VKQ27_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLY88783.1"
                     /translation="PGSDGIVPDIATARDVASRIGYPVLIKASAGGGGRGMKVAREAS
                     ELEDAFRIARTEARAAFNNDAVYIEKYLDHPRHIELQILADGRGSVVHFGERDCSLQR
                     RHQKLLEEAGSPALSPAGRDSLGATATAALAKIGYRNAGTLEFLYQDGQFSFIEMNTR
                     LQVEHPVTEMVCGIDLVREQIRIAAGAPLGYGQADIHFTGHAIECRINAEDPATFMPT
                     PGRVTAFHAPGGLGVRVDSALYAGYFVPPYYDSMVAKLIVHAPTRIDAINRLRRALDE
                     FAVVGIQTTIPLHQRIVDDPEFQRGDYTIHWLERFVAR"
BASE COUNT         2375 a         4040 c         4939 g         2329 t
ORIGIN      
        1 gccgaacaac cctccgcgcg catggtgcgt catgccatca taccgtgcta ggaacgcaac
       61 cctgatctgc atgggaggag gagccgcaca tggccatccg caagctgctg ctgccgctga
      121 ccggcaccgc tgccggcgaa gcggccttga ctacgagcct tatgatcgcc cgcctgtgga
      181 acgcccacgt caccgcgctg catgtgcggg tagacagccg cgacgtcgcg ccgctggctg
      241 gcgagggact gtcgggcgcg atgatcgagg aaatgatgtc cgccacagag aaggaaagca
      301 gcgaccgggc gcatgccgtc cgctcgatgt tcgaccggtt cgtggcgcag cacgacgtga
      361 tcctgtccga ggcgcatccg ggaacggacg ccgcgaccgc cagcttcgcg tcggtgacgg
      421 ggcgcgagga agatctggtc gctcagcagg cgcggctggc cgacctgacc gtggtgccgc
      481 atccggatgc cggcgaggac gtgtcatcgt ctgacgcgct gcatgccgtg ctgttcgatt
      541 cgggtcgtcc cgtcctgatc tcgccgcgca ccgcgcctac gaccatcggt tcgcgcctgt
      601 gcctgggttg gaacgggacc gcggagtcgg catccgccgt gtatgcggcg ctgccgtggc
      661 tgaaacgggc ggaggctgtg cgtatcctgt cggcggaggg atatcagcgg cgaggccccg
      721 cggcgcctga gctcgccgca tatctggcac tgcatggcgt gcatgcggac atccacatgt
      781 tccagtcggt aggaaactcc gtgggcgccg gattgcttgc cgcggcacgg gacttcggtt
      841 gcgacctgct ggccatgggg gcctattccc attcgcggct gcggcagctg atccttggcg
      901 gggtgacacg tcacgtactg gagaacgccg gtctgccggt gatgatgaac cgctgagttg
      961 tccgaggcgc tcgacgccta tgtgaaactg ctgcgcgcca accgggcggt actggcgcgc
     1021 gtggaacccc ggctcgtggc gtcagggctg acgacgacac agttcggcgt cctggaagcc
     1081 attctgcaca aagggccgct cagccagcgc gatctgagcc tgaaggtgct caccagcccc
     1141 ggcaatatga ccgacctgat cgacaagctg gaggcgcgcg ggctggtccg acgcgtgcga
     1201 cagaaagccg atcggcgggc ggtgcgggtc gagctgacag tggctggggc ggcgctgatc
     1261 gagccactgt tcgccgcgca tgccggcgat atcgacgccg cgatggcggg gctcgacggg
     1321 gacgagttgc ggcagctggg cactctgctg cggaagctgg ggctcgcggc gggaggggtt
     1381 gaagactgat gggacgcgca ctacttcatt gtcgaatcgt tcaatatcga acgaatggcc
     1441 aagaggagga tcagcgatga tcgaggtgag gcccttcggg gaactcggcg cattccgtaa
     1501 tgagtggctg aacgcgaagc accacttcag ttttggcggc taccacgatc ccaagcgtat
     1561 gggcttcggg cggttgcgcg tctggaacga tgatgaggtt gcggcgggaa ccggtttcga
     1621 tccgcacccg caccgcgaaa tggagatcgt gacctacatc cggcagggcg cgatcacaca
     1681 ccgggacaac cttggcaacg aggggcggac cgaggcgggc gacgtgcagg tgatgcacgc
     1741 cgggaccggg atcgtgcatg cggagtacaa ccgcgagaag acgccgaccc ggctgttcca
     1801 gatctggatc cagcctgacc ggcatggcgc gaagccaggc tggtcgacga ggcagtttcc
     1861 gcgcgagggc ggactgtcgg tgctcgcgag cggacgggcg gccgatgcgg agagcggcgc
     1921 tttgtcgctg aacgccgacg cggcggtact ggcagggacg ctggcggcag gcgagacggc
     1981 gcgggtgacg cttgagccgg ggcgcaatgt gtatctggtg ccggcgaccg ggacggtaga
     2041 agtgaacggc gtcaaggtga gtacacgcga cggagcggcg atcagcggtg aggccgagct
     2101 gacgatcgcc gcgacggagc cgagcgaggt ggtgctggtg gacgttgcgt cttaggtcgc
     2161 ttcttcgtgg cactcggaat ggtgggcgcc gtatagttgg cgcccatcgc cttcggaggc
     2221 ccctgtgcgt ttcccacttc gtggcttggt tttggcgtcg ctgctcgtgc ctggcgtcgc
     2281 ctctgcgcag aacgtgacgc aggagcaggc ggctgccttg cgtcagcaac tgaccgacac
     2341 gctgtctgag ttgctcggtc ctgcggtgaa gctgcaggat ctgccgctga agatcacggc
     2401 gaacggcgac cattacgata tgtcgttgcc gatcccggga ttggaagtga ccacaggcga
     2461 tccggccgtg acggcggagc tgaagccgct ggatggcgga cggtggtctc tggagaaggc
     2521 gaggttccca agcacgggca atttcacctt cacgctgccg gacaccagcg ccgggccggg
     2581 cggaccgatg aaggggacct attcgatcgg caagcaggat ctccagtcga cgatcgaccc
     2641 gagctttgcg tcgccttcga cctctcattc ggcggtggaa gcacttgcca tcaccatgga
     2701 tggaccaagc aagcaccagg aagagcatat cgaccggatg gttggtgagg ccgtgttgac
     2761 gccgacgaag gacggccggc tcgatctgac ctcggatgcg accgcggagg gtttgaagtc
     2821 ggctgccttg gaggacaaca agccggcgat tgcattcggc gcgaagcggg tgcacgcgac
     2881 gactcacgcg aagggaatca accgcgagca cgtcgccgaa ttctggggcg cgttcgtgaa
     2941 attcatcgcg ctggtaccga agccgcccga gaagaaagag ggcgagaccg agctgcctcc
     3001 tctggcgaaa gcgcaactgc ggctgatgat cgcggcgatg caggacacgg tatcctcgat
     3061 cagcatgcag gagaccgtgg acgatatgca gatcgagatc gccgacaagg gcggggcaac
     3121 gatcaagcaa ctgcagttcg gatttggtgg cgaggcgccg gacggcagcc tgcatacctg
     3181 gctggatctc gggttcgaag gcgtggaaag cccgacgatg ccaccgaagc tcgcgaccta
     3241 tctgcccaag catttcgaga taaagccgac gctgtcgggc gtgccggtgg cggtgctgca
     3301 gaagctcgcg atggacgcga gcgatgacga tccggcgaag catccggtgg gccccgatct
     3361 cgcggcgatc tttgccaatg gcggggcggt gatcgggctc gagacgctgt cgttcgacct
     3421 ggggccggcg acgctgcgcg ggacggggaa ggtgacggtg acctcgccct cgagctgggt
     3481 gggtgatgcg catgtcgtcg cgaccgggtt cgatgagctt gcgacgcagg cgcgggaaaa
     3541 ccccgacctg caacaggcgc tgccggtgct ggtgatgatg cgcgggctgg cgaagccgga
     3601 tggcgacaag ctggtgtggg atatcgcgag cgaaggtccg aagctcacgg tgaacgggat
     3661 cgacatgtcg gcgctgaccg gcggcggtgg cgacaagggc aagccgccac atcctcctca
     3721 cccgggggcc aagccgtaga gcgtcacttc acgttccgtg cggtcctcga gctcgaggct
     3781 ggtgggcacg gcgtaggtgg caaagggcgt gaggccgtcg cgcggcagat aggcgcgccc
     3841 gggatcggcg atccatacct cggcctcggt ggcgagggcg cgcagccacg ggaggatgtg
     3901 gctggtcatc ggcgcctcgt agcagacgtc gccgcacagg atgagatccc agcgggaccg
     3961 gccgccgacg acgtcgctgg tggggagggt gagcgctatg ccgttcgcgg cggcattcag
     4021 cgacagcgcg gcgcgggcaa gcgggtcgat ctcggcggcc tcggcggatg cggcgccggc
     4081 ccgcatggct gcgatggcgg cgatgccgca gccggcggcg aaatccagga tgcggcggcc
     4141 ggcgacggtg gcggggtgat ccaggatgta gcgcgcgagc gcctgaccgc caggccaggc
     4201 gaaggcccag aatggcggtt cggcgttgtt gccgtggcac caggtctcgg tggcctgcca
     4261 gatgggcgtg atctcggtgg caagctggag ggtgatctcg ggcaccagtg gcgcggttgc
     4321 ggcccgtgtc tggccaagaa tgaaagcggc gtcgtcacgg acgagtgggg acattccggg
     4381 gtcatagcag aacgccgcga ccgggtgggc cggtcccgtt ggtttcagcg gtgcgttgta
     4441 cgacgcggat cagggcgggg cggtggcgac agcggcggcc gggtcgagcg gattgacggg
     4501 cggctcgccg atgaagaagc cgagagcctg gcgcatcgcc tcccggctca tgttcaccca
     4561 atcccaggtg gccttggggt tgcgatcctg ctcgttggaa aatgcctgac ggcggcgggt
     4621 tttctcacgc atcagcttgt cgatgctgcc gccgaacaag gtcatgagct cgaacagctc
     4681 gttccagaag gcgctatggc agaagccggg gacgacggcg aggtcgaggc agatatcgta
     4741 gatggtgcgg ccgagggggc gccggcgcgc caggcattcg aggtcctcga gggttggcat
     4801 gaagagctcg gggtcgtccc agccggcagg gcgggatggg cgcggtgcgc gctggcgggt
     4861 ggggatgggc gcgacttgct ggctgggctg ggtgtcggct ggggcggtgg cggcggctga
     4921 cgcgcgggtg cggcgcagga cgaagtcgac gtcttcgccg gcggcgacgc gggcgagcag
     4981 aacgcgctca agcgcttcgg cgcgcaggat gccacggtgg aggtgggcga ggatggtggc
     5041 gagcctggcg gtgccgaagt tggcggcgat ggcgtggaag ttgggagcgg tggcgcgctg
     5101 gcggacggta tcgagcagat ggcggccgta ggtgagcaga gtgccgacgg cgtgcaggaa
     5161 gatggtgatg ttgctgggga ccgtggagtc gcggttgtcc tgtgggggat ccggagggga
     5221 ggcgacaggt ctttgatccg cggcaaggtt catgtctgac aaactaacag gataactaac
     5281 agagaggcaa ggatgatttt tctcaatggg tcctgtgaca gtaagcaatc gctgtatcca
     5341 aataacggct gcgctcagcg ccgcgcctct ttcagtgtgc gccgcaaatt gcttccgaaa
     5401 gtctggatgg ttagcccctg cacgggccgt ggatcacgaa ggtgacgcgc tcgcgcacac
     5461 ctaggccttc gagcagcgct tcaaggtagc ggcggtgctc gacgaagcga tacgagccgg
     5521 gaccgctgtt cggcagtttg gcggagtcgc ccatgccgat caggtctggc gcgatgcaac
     5581 gcccaagcgc tgcaggtgtg gcagcacatt gcgccagaga tacgatgaag tcgggttgcc
     5641 gtgcagcagg acggtagggt cgccctgtcc gacctcgaca tacgccatct cgctgtccag
     5701 gacgcgccgg cgctgcttct ggtaagggaa tgccgcagag atcatgtcgc ttctcccaag
     5761 tacaacgcgc tgagtttggg aagctggcga ttaggattcc tagggactgt tcttactttt
     5821 tctaaagata gagagccgcc gactcatggg gcgcgcagaa atggccaggt gattactttg
     5881 gaatagaggc cttcgggcgc cgggcaaggc gcgtgacaaa gcggatcagg tcgccgcgat
     5941 agtgcagctt ctcgtaatcg actggctgat tgccggcgct gtaggaggtg cgctcgatca
     6001 ggaagatcgg gctgccgacc ggcacctcga gcgcctcggc ggcaacagaa tccgcggccg
     6061 ccgcctcgag cttgtattcg gcttccagca gaggggtttc gtacttgttt tcgagtaggg
     6121 cgaagaccgg ttcagcggcg agatcgttcg ccgcgatttt ctcccctagc acacgcggca
     6181 gataggtctc gtcgaaggac atcggcacac catcggcgag gcgaacacgg cgaagcctga
     6241 cgaccggcgt gccaggtgcc agtcccaggt gctgcgcgac gggttcgtcg gccgagacga
     6301 tgcgcttgtc gagcaagcgg gcagtcgggg ttcgccccag ggcctgcata tcctcgacaa
     6361 accccgtcag ctcggtcagc tcctgtgtga tcttcggctg ggtgacgaaa gtgcctttgc
     6421 ccctgcggat ctcgagcagg ccgcgttcca ccaggttctg gatcgccttc cgaaccgtgg
     6481 tacggctgac cttgaagcgc tcgatcaggc cgtcttccga cggcaattgc gtctccggcg
     6541 acaggttgcc gtcggcgatg tcgccggcca ggacggattc gacgcgctcg tagaggggag
     6601 atcgcgacat cacgacaaca tgacgtcatt atgtcttgcc gtccagcgcg acgtcgccaa
     6661 ctgccggaac cgtgagggcg gcggtttgcc ggcgccccca cagggatcac cgaagcagag
     6721 gacacgatgc gccgcattca tggacggtca acggaatggg gagacctgct caccggccgg
     6781 atcggcagtt ccgccttcgc ttattgggcg ccgtttccgc tgcggctgat tgtcggatat
     6841 ggcttcatgg agcacggatt tgccaagctg gcccgggggc ccgaggcatt ccccttgatc
     6901 ctacaggcgc ttcatgtgcc ggcgccgcat ttcatgggat ggctcaccat tgtggtcgag
     6961 atattcggcg gcttggcggt gcttgtcggt gcgctggtgc cgcttgccag catcccgatg
     7021 gcggcggtcc tgctggttgc gatgttcacg gtgcacctgc agtacgggtt cagctcgatc
     7081 aaactccagg cggtgaccgc ggcgggggcg caattcgggc agccggggtt cgagaccgat
     7141 ctcctctacc ttgcctgcct tgcggccctt gtcctcgggg gcgccgggcc attggcgatc
     7201 gacggtctca tgacaaggcg cagagacagg cacaggcttc tcggctgaac gaccgcgatg
     7261 acgatgcggc cccggagcaa agcgtcctcg cattccatga accggtcatg cccagccgat
     7321 gaggatggcc agcccggcag caagcccggc ctcgcggttt gcccggaaca ggcgcaggca
     7381 cagcgccgga tcgttgatgt cgagttgcat gacctggcgc gccagcagcg cagcaggcaa
     7441 cagcaatgcc ggatagaacc aggggccgag tccggtcagc catcctgcta tgccgagcag
     7501 aatgatcgtg ccggcgtagc aggcggcgag gaacggcgcg gtatgctcgc cgaaaaggcg
     7561 tgcggtggag ccgatgccga caagggcatc gtcctcgcgg tcctgatgcg cgtagatcgt
     7621 gtcgaagccc aggtcccaga ggattgcggc gccgtacagc gtcgcccagg cagcaccgag
     7681 atgcccggcg cccgcggcgt agccgagcgg tgcgccgaag ccgaaggtga agcccatcat
     7741 gagctgcggc caccaggtga cgcgcttggc aagcggatag agcgcgacca gcagcagcga
     7801 tgccacgccg aggatctgcg cgagccggtt gagctgcagc aggatcagca ggccggccag
     7861 caggaggatc accaggaata ccagcgcatg ccgcgggcgc agcatgccgg aggccagggg
     7921 acgccccgcc gtgcgcgcca cttggcagtc gaggtcgcgg tcccacagat cgttcaccac
     7981 gcagccggcc gagcgcatca ccatgctgcc gatggcgaac agcaggatca ggcggatggt
     8041 ttcggcgggg gccgggccgg cgagcaggat gccccacagg ccaggcagga acagcagcca
     8101 ggcgccgatg gggcgatcga cccgggcaag taacaggtaa ggcaaccagg agcgcggcag
     8161 ccgtgccacc cagccggtcg cgacgatgtc ggtgtgcgcc atggctgcta ctgtgcgcgg
     8221 catcatgacc gggtcaatcc gcctgtttgt cgatgcgccg ctggcggttg gggaaacgat
     8281 cgcggggtcg gcaggccagg cacattatct cggcactgtc atgcggcgtg gcgtgggcga
     8341 cgcagtgctg ctgttcaacg gctgtgacgg cgagttcgct gcccgcatcg cgctgctgcg
     8401 acgggaccgg gtggagttgc acatcgagcg gttgttgcgg gcgcagtccc aggagcccga
     8461 tctgtggctc ctgttcgcac tgctgaaacg cgatgcgaca gacctggtca tgcagaaggc
     8521 aaccgagctt ggcgtctcgg cgctgcatcc ggtgataacg gaacgctgca atacgcacag
     8581 gatgaacgcg ggccggttgc gtgccatcgc ggtggaggcg gccgagcagt gtgaacgact
     8641 gactgtgccg gtgctgcacg aaccgcggcc gttgcaggaa gtgctggcag attggtcgcc
     8701 cgaccggcgg ctagttgctg cactggaacg cagcgatgca ccgcggatcg cagccgtgcg
     8761 cggccagacg gcattgctgg tggggcctga gggcgggttt tcgccagcgg agcttgaagc
     8821 gctgcgggtg catccctttg ttacaggcgt ctcacttggt ccgcgtgtac tgcgggctga
     8881 caccgcctgc atcgccggcc tggctctgtt gcagggggga gactgcggct agtaccatcc
     8941 aacgcaacct gttacgcccg gagcgacatg tccaatcccg gagatgccga cgcgacaccg
     9001 attacgtcgc tcaaacaact ggctgattac cttgctgccg gctgcaagcc ggcggagcag
     9061 ttccgcctcg gcaccgagca cgagaaattc ggatttcgtc tcgccgatct gaggacgcca
     9121 ccttatgaac cggctgatgg ccagcccgga tcaattcgcg gcctgctgga ggggctgcag
     9181 ggtttcggcg gcacgccgat actggatcgc ggcgcgatca tcgggttgaa gcagggcgac
     9241 gcggcaatct cgctggagcc cgcggggcag ctcgaattgt ctggtgcgcc gctcgaaaca
     9301 ctgcatcaga ctgcagcaga actggaagcg cactatgacc aggtgcgtgc ggtgtcgaac
     9361 gaactggcga tcgggttcct gccgctcggt ttccatccgt tggccaagcg cgcggatatg
     9421 ccgtggatgc ccaaggggcg gtacgcgatc atgcggcgct acatgccaga ggtcggaacg
     9481 cttggcctcg acatgatgac ccgcacctgc acggtgcagg tgaacctgga ttacgcgtca
     9541 gagcaggaca tggtgcgcaa gctgcgcgtg tcgctgctgt tgcagccact ggcgactgcg
     9601 ctgttcgcca actcaccgtt caccgagggg cggcccaatg gcttcctctc gtaccgggcg
     9661 catgtctgga ccgacacgga taaccagcgc tccggcatcc ctcgggtgat gttcgaccct
     9721 gggtttggat tcgagcgata tgtcgaatgg ctggtcgatt cagtgccgat gtatttcgtc
     9781 tatcgggatg gtgcgtacat cgacgtggct ggcgcatcct tccgcggcta tatgaacggc
     9841 caattgcaca acgtggcggg ggcggtcgcc actgtgggcg atttcgccga tcacgtaacg
     9901 acagtgttca cggacgtccg gatcaaacgt ttcctggaaa tgcgtggtgc cgatgccggg
     9961 cgggcggata tgatggtggc gcaggccgct ttctgggtgg ggctgatata cgacgacgcg
    10021 gcgctggctt cggccgaggc gctgttgcgc gatgccacct gggaggaggc tgttgcgctg
    10081 cgtgcggcag tgccccggga agggctgggt gcgtcgtggc ggaatggcgt gctgcgcgat
    10141 cttgcgcagg atgtggtggc gatcgcccgg gatggcctgc gttcacgcgg caggctcgac
    10201 ccgtcgggcc gggatgaggc gatgtatctc gatccattga tggcaatcgc cgccggtgag
    10261 ccaacccagg cagaacattg gctggcgcga ttccatcagg cgtggcaggg tgatgtccga
    10321 agaatttatt cggaaggggc aatctgaaag ggcggcgtaa cctttcgcgt gttcaagccg
    10381 ggttctggta acgaccacct ccgcagatat gacgcaatga tggcaggcgc ttcacaccga
    10441 ccacccgccc acctgacccc gccaagttcg gccggtcagg tcgtagttcg aatgcgtctg
    10501 atcggccaga tggaagcctg gacgctaacc agcgaaaatg tgttgccagc agggcgcaaa
    10561 acgcgtgctc tgctcgccgt tattgcgctg tcatcgccgc gtcccgcatt gcggggccgc
    10621 ctcgctgaac tgctgtggag ccggcgtccc gaggaacagg cccgggcctc gctccggcag
    10681 gagatccacc ggctgctcga gtcactggcg cccgctggaa ctgaaatatt aaccgttacg
    10741 cgcgaccatc tgtcgctgcg gccgggtctg gtctggatcg atgtcgaaga agtgatgcgg
    10801 gcgacgaccg accagccggc ttcgctgtcc ttgctcgacg gtgacctgct ggaagacctg
    10861 gacggaatcg acccgacatt cgacgcctgg ctgacgacgg aacgcgagcg actgcgcgac
    10921 cgcgccagga acgtggccga ggccctgctg cgcgagcagg tcgagccgga ggcggcgatc
    10981 cccgcggcgc agcggctgct gcagatcgac cgtgcccacg agggcgcgtg gcgggcgctg
    11041 atgcgcgccc atgccgcgcg cggcgagcgt ggcatggcga tccaggccta cgatcgctgc
    11101 cgggcggtgc tcgccgatct gctggatgcc gcaccgtcgg cggaaacgca aaagctgctg
    11161 gcagaaatcc gggggccttc cggcagccgg atgccactgc gtccccctgc gccgccgccg
    11221 ctgccggcac cgcagatggc ggccgttgtc gaggaaccgg aagagccgcc cgcggtgctg
    11281 gaaccgcgcg cacaggaaat ccgtccgacc cgcggtggcg cgcatgtcgg cgtgatgccg
    11341 ctgcaacttg tgggaacgtc cgacgaagag gcgcatctgg caccaggcct cgccgaagag
    11401 ataaccacgg cactggcccg gtttcgctgg atattcgtgg tggcttcgaa ttcgctggcg
    11461 cggttcgccg ccgatacgcg ggatgagaca gcgatccggc gcacattcgg catcgacttc
    11521 ctgctggacg gctcgatcca gaaggtgcgc aaccggttgc gcatcaccgt gcggctgctg
    11581 gacctgcggg ctggcaatca agtcgtctgg gcgcggcgtt ttgaccggca gaccaatgat
    11641 ctgctgtcgc tccaggatga gattgcgtcg gaagtcgtcg cgcaaatcga tccggaaatc
    11701 ctgctgatcg aggcgaagcg cagcgcaagc cagccgccgg tggatgccac cgcctatgat
    11761 ctggtgttgc gggcgattcc gctgatcggg cggatggagc gcagccgcta catcatggcg
    11821 ggcgagtacc tggtgaatgc gatcgcgctg gagcccgact atgccgcggc gcacgcctgg
    11881 tacgcctact ggcacatctt cctggttggg cagggctggg ccgaagatcc gcacgcgatg
    11941 atggaagagg ccggcgaact ggctgagcgt gccgtcgtgc tcgacccatt cgatgcgcgc
    12001 gcgctgacca tcgcgggaca tgtgcgggct tatctgcacc ggcggctgca tgaggcggcg
    12061 gcgctgcatg agcgagcgct gtcgctcaat ccgaatctgg ccatggcgtg ggcgctgtcg
    12121 gcgatcacct atgcctatat gggcgaggcg gaggaagccg agcggcgcaa caaccgctac
    12181 aagaagctgt cgccgctgga cccgcatgca ttctttttcg atgcgttctt catcctgatc
    12241 cacctgatga agcgtgatta tgagaccgcg gtggcggtgg gacgcgcggt cagcgagatg
    12301 aacccgtcgt tctcggcgac gtacaagcca tatctggctg cgctggggca tctgggccgg
    12361 acccaggagg ccgcggtggt gcggcgtcgg ctgctggcag tcgaaccgga tttcaccatt
    12421 gagcggtttg tcgcgacgtc accgctggag cgggaggctg accgcgacca ttacgcgcat
    12481 gggctccggt tggccggcgt gccggagcgc gccgcggagg tggcggagca gaacctgcag
    12541 ggacaggaag cgcactagag cgtgatgacc agaggcggaa tcgccggagg tttgaatcac
    12601 gcgataatgc gaagggcctg agcaagatct gcgagtcctc gagcgctcat cccgctctaa
    12661 gcgctgtccg caacggccgt tgccgatgca aacggtccaa ttcccacact cgccgcggga
    12721 aggaactgga cgcggatcag cgggcgacga agcgttccag ccagtgtatc gtgtagtcgc
    12781 ctcgctggaa ctccgggtca tctacgatgc gctggtgcag cgggatcgtc gtctggatac
    12841 cgacgacggc aaattcgtcg agcgcgcgac gcaggcggtt gatcgcgtcg atgcgcgtgg
    12901 gcgcgtggac aatcagcttg gccaccatgc tgtcgtagta aggcgggacg aagtagccgg
    12961 cgtaaagggc tgagtcgacg cgcactccaa ggccacctgg ggcatggaag gcggtcacgc
    13021 gacccggtgt aggcatgaat gtcgcgggat cttcagcgtt gatgcggcac tcgatcgcgt
    13081 ggccggtaaa gtgtatatcg gcttgtccat acccgagcgg cgcgccggcg gcgatgcgga
    13141 tctgctcgcg gacgaggtcg atgccacaga ccatctcggt gacgggatgt tcgacctgga
    13201 gccgggtgtt catctcgatg aaggaaaact ggccgtcctg ataaaggaac tccagcgtgc
    13261 cggcattgcg gtagccaatc tttgccaggg ccgcggttgc tgtggcaccg aggctgtcgc
    13321 ggccggcagg gctcagtgcg ggggagcctg cctcctccag cagcttctga tggcggcgct
    13381 gcagactgca gtcacgttcg ccgaagtgga ccacgctgcc gcggccgtcg gcgaggatct
    13441 gcagctcgat gtgccgcggg tggtcgagat acttttcgat atacacggcg tcattgttga
    13501 aggcggcgcg ggcttcggtg cgcgcaatgc ggaaggcatc ctcaagctcc gaggcctcac
    13561 gcgcaacctt catgccgcgg ccgccgccgc cggccgatgc cttgatgagc accgggtagc
    13621 cgatacgcga ggcaacgtcg cgggcagtcg cgatgtccgg cacgatgccg tcggagccgg
    13681 gaa
//
LOCUS       DATGEM010000232         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1622673, whole genome shotgun sequence.
ACCESSION   DATGEM010000232 DATGEM010000000
VERSION     DATGEM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622673"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VKQ27_07345"
     CDS             <1..413
                     /locus_tag="VKQ27_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HLY88784.1"
                     /translation="TYRASGAGGQHVNKTESAIRITHVPSGIIVACQTDRSQHRNRAT
                     AMAMLKARLYEAELQKREAAAAAVEDAKTNIGWGHQIRNYVLAPYQLVKDLRTGVEKG
                     NPDAVLDGDLDAFMAAALAARAGATRSEASAQAR"
     gene            527..1165
                     /locus_tag="VKQ27_07350"
     CDS             527..1165
                     /locus_tag="VKQ27_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88785.1"
                     /translation="MSRPVRTVHATSKGEIDHALATADRIIVEGDEELLSYAIDRAAV
                     EPEDRITIELPSRAARLPREPDEAPALRPAPVPTPVPRALGRANGRPREGVRQSTSFV
                     AVAAGSIVGLGIVGAVVWLIVPGRRYAPNAVIEPHSNITGHEATDDFWANLPNVLWPV
                     VATVAILALFLIARQAISSGNNVTIAWRVTEKVQGRVVITKIRDRTPGRRAA"
     gene            complement(1171..3594)
                     /locus_tag="VKQ27_07355"
     CDS             complement(1171..3594)
                     /locus_tag="VKQ27_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLY88786.1"
                     /translation="MSGPERMLSPGSFLGDGAAQLVVAIMVSLAGSGLATWGMSTTAD
                     NLRCKVSRVALSAMLLASTAWLVFAFALRWRFPSFEPEIPWPAMLQSWAIVGAGAVSC
                     VLIVCIGRPSVRNVALSGSILSSAVSCMVFASMSGLAAPSALAYDLSLVVAAMAVCST
                     LAATGLWLLHRSQRLRHLSGLLLGTAFPLLTILSLMSVLPFSEWQMAAATPGAMAFRP
                     VIVVFLSELGVSVALGLLGTGVDRQIATQFARENERLRQLSESTFEALVIHRDGTVLD
                     ANSAFCSLIGRSLSEIQGEPVARFVPAAKELSLVSPVTGRPEPLEIEIAGADNTPLPV
                     ELLSREIPYARGAARVTALRDIRERRAAEQRIWYLAQHDLLTGLPNRAHFNDVMTRQL
                     ALSKRDGIPLAVFCIDLDRFKHVNDTLGHAAGDLLLKQVADRITGIIREGDTLARIGG
                     DEFVLLQTGAAQPEGSAQLAQRVIDRLTEPFDLNGSSATVGASIGVALAPQDADKADE
                     LVSYADIALYRAKANGRGAYCFFKTGMDTLLRQRRELEQGIAHAISVGGFTLDFQPLF
                     SGEATDRLIGFEALLRWPDPVRGMIPPADFIPLAEECGLIVPLGAWVLQAACHEAVSW
                     PEPWRVAVNVSPRQFSTGDLVATVREVLDRTGLDPARLEIEITETLLITDSDAALRTL
                     QNLKRLGVRIVLDDFGTGYSSLSYLQRFPFDKVKIDRSFVTGLVTNDNARAIVNAILA
                     MSHQLHLEVTAEGVEQEAQLSILRSSHCDQIQGFLLARPIPQHELPRFIEYRGDAGQD
                     RVDEAALSH"
     gene            3729..4271
                     /locus_tag="VKQ27_07360"
     CDS             3729..4271
                     /locus_tag="VKQ27_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88787.1"
                     /translation="MSAWKLICRGVRCWGFIAALMAATRPAGAAAPSLSSQDLQVLGS
                     ALTFVEPRPAGTLAVVYSAADEASRQDAAAIMTALGKGLPIGGAILTPTLVDAAALGS
                     TDFGLAIIAANANGEAVARSVDARHALCVTADRPAVQRGLCTVAINSAGRVDIFLNNR
                     VAQRAGITFATAFRMMVHEQ"
     gene            4268..>4667
                     /locus_tag="VKQ27_07365"
     CDS             4268..>4667
                     /locus_tag="VKQ27_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HLY88788.1"
                     /translation="MKYWLSCVALCVTIIVVAALSVSPRAQQLDYGTLEKVFHEPVTT
                     SATGQPQRASEAPVNMTIITQDDIRRSGATTIPDALQYVAGVDVRRYGIADEEVGIRG
                     YNAAYNPRLLVLVNGRQVYSDDYGRVFWPTV"
BASE COUNT          880 a         1455 c         1539 g          793 t
ORIGIN      
        1 atacctatcg tgcgtcgggc gcgggtgggc agcacgtcaa caagactgaa agcgcaatcc
       61 ggatcacgca tgttccgagc ggcatcatcg tcgcgtgcca gacggaccgc agccagcacc
      121 gcaatagggc gacggcgatg gcgatgctga aggcaaggct gtacgaggcg gaactgcaga
      181 agcgggaggc ggccgcagcg gcggtggagg acgccaagac caacatcggc tggggacacc
      241 agatccgtaa ttacgtgttg gcgccgtatc agttggtgaa ggatctgcgg accggggtcg
      301 agaagggcaa tccggacgcg gtcctggacg gcgatctgga cgcgttcatg gcggcggcgc
      361 tggcggcgcg agccggtgct acgcgcagtg aggcgagcgc gcaggcaagg taactcttgc
      421 gcactcgagc agcggcggcg cgttcgatcc gcttcatggc gcgaagctgt gcaaccgcct
      481 tgcaggaggc gtaacagtag gtccgcacca gctcaggacg ctgcccatgt cacgccccgt
      541 gcggactgtc cacgcgacct ccaaggggga aatcgaccac gctctcgcga ccgcggaccg
      601 gatcatcgta gagggcgatg aggagttgct ttcctatgcc atcgacaggg ctgcggttga
      661 accggaagac cggatcacca ttgagctgcc cagccgagct gccagactgc cgcgcgaacc
      721 ggacgaggcg ccagcgttgc ggccggcccc cgtgcccacc cccgtcccca gagccctggg
      781 aagggcgaac ggacggcctc gagagggtgt ccgccaatct acgtcgtttg tcgctgttgc
      841 cgcaggttcc atcgtcggcc tcggcatcgt cggggcagtt gtctggctga ttgtgccagg
      901 gcggcggtat gctccaaacg cggtcatcga gcctcattca aatataactg gccacgaggc
      961 aacggatgac ttctgggcca acctgccgaa cgtgctgtgg ccggtggttg ccactgtcgc
     1021 aatcctggcg ctgttcctga tcgcgcggca ggcgatttcc agcgggaaca acgtcacgat
     1081 cgcctggagg gtgacggaaa aggtccaggg acgcgtggtg atcaccaaga tcagggaccg
     1141 gaccccgggg cggcgtgcgg cctgagacgg tcaatgcgaa agtgcggctt cgtcaacgcg
     1201 gtcctggccg gcatcgccac ggtactcgat gaaccgcggc aactcgtgct gcgggatcgg
     1261 cctggccagc aggaaaccct ggatctggtc gcagtgactg gagcgcagga tcgatagctg
     1321 cgcctcctgc tccacacctt cggcggtgac ttcgagatgc agttgatggc tcattgccag
     1381 gatcgcattg acgatcgccc gtgcgttgtc gttcgtcacc agtccggtca cgaacgaccg
     1441 gtcgatcttg accttgtcga acgggaagcg ctgcagatac gaaaggctcg aatagccggt
     1501 gccgaaatcg tcgagcacga tacgcacccc gagccgtttc aggttctgca gcgtgcgcag
     1561 cgcggcgtcg ctgtcggtga tcagcaacgt ctcggtgatc tcgatctcca ggcgcgccgg
     1621 atcaagaccg gtccggtcca gcacctcacg gacggtggca acgagatcgc cggtgctgaa
     1681 ttgccgcggc gagacattga ccgcgacacg ccacggttcc ggccaggaca cggcttcgtg
     1741 gcatgcagcc tgcaataccc atgcaccaag cggaacgatc agaccgcatt cctcggcgag
     1801 gggaatgaaa tcggcgggag ggatcatgcc gcggacggga tcgggccagc gcaacagtgc
     1861 ctcgaacccg atgagccggt cagtcgcctc gccggagaac agcggctgga agtccaaggt
     1921 aaagccaccg acgctgatcg cgtgtgctat gccctgctcg agttcacgcc gctggcgcaa
     1981 tagcgtgtcc ataccggtct tgaagaagca gtatgcgccg cggccgttcg ctttcgcgcg
     2041 atacagcgcg atatcggcat agctgaccaa ttcgtcggcc ttgtctgcat cctgcggcgc
     2101 cagagcgaca ccaatgctgg caccgaccgt tgcgctgctg ccgttgagat cgaacggctc
     2161 ggtcagccgg tcgatcaccc gttgcgccaa ctgggcggag ccctcgggtt gcgccgctcc
     2221 ggtctgcagc agaacgaact cgtcaccgcc gatgcgtgcc agcgtgtcgc cctcgcggat
     2281 gatgccggtg atgcggtcgg ccacctgctt cagcagcaga tcgccggcgg catgtcccag
     2341 agtgtcgttg acatgcttga accggtcgag atcgatacag aacaccgcca gcggtatccc
     2401 gtcgcgcttg gaaagcgcca gttggcgcgt catcacgtca ttgaagtggg cgcggttcgg
     2461 cagcccggtc agcagatcat gttgggcgag ataccatatg cgctgttcgg cggcacggcg
     2521 ttcgcggatg tcccgcagcg cggttacgcg ggcggcacct cgtgcgtacg gtatttcgcg
     2581 cgacagcaat tcgaccggaa gcggcgtgtt atcggcaccg gcgatttcaa tctcgagcgg
     2641 ctcgggcctg ccggtgacag gcgaaacgag tgacagctcc ttcgccgccg gcacgaagcg
     2701 agcaaccggt tcgccttgga tctcggacag ggagcgtccg atcagcgagc agaatgcact
     2761 gttggcatcg agcactgtcc cgtcgcgatg gatcaccagg gcctcgaacg tgctttcgct
     2821 gagttgtctg aggcgttcgt tctcgcgcgc gaactgtgtc gcgatctgac ggtcgacgcc
     2881 ggttcccaac aatcccaatg cgacgctcac gccgagttcg gacaggaaca cgacgatgac
     2941 aggtcgaaac gccatggccc ctggagtcgc ggctgccatc tgccattccg agaatggtag
     3001 taccgacatg agcgacagga tagtcagcag cgggaatgcg gtcccgagca acagcccact
     3061 cagatggcga agacgctgcg aacggtgtaa cagccacaat ccggtggcgg ccagagtgct
     3121 gcacactgcc atcgctgcga cgaccaggct gaggtcgtag gcgagcgccg aaggggcggc
     3181 gaggccgctc atgctggcga acaccatgca tgagaccgcg gacgacagga tcgagccgga
     3241 caaggcgacg ttgcgcacgc tcggtcggcc gatgcaaacg atgagcacgc aggacacggc
     3301 gccggcgccg acgatcgccc aggattgcag cattgcgggc caggggatct ctggctcgaa
     3361 actggggaag cgccaacgca gcgcgaacgc gaacaccagc catgccgtgc tcgccaacag
     3421 catggcggac aatgcaacgc gcgacacctt gcatcgcagg ttgtccgccg ttgtcgacat
     3481 gccccatgtt gcaaggccgc tgcctgccag gctcaccatt atggcgacga caagctgtgc
     3541 agcgccgtca ccaaggaatg atcccggcga gagcatccgt tcaggtccgc tcaactggca
     3601 tccgttatag gcggtcggtg tgaatgaagt gctaccgcaa attcgttggt atgtcggctc
     3661 agttcatcaa ttcctaatcc gctgctgcta gcacggaacc tgcagttccg ggggcgtgct
     3721 gtcggtagat gtcggcctgg aaactcatat gccgcggcgt gcgctgctgg ggcttcatcg
     3781 ctgctctcat ggcggcgacg cgaccagcag gtgcggcggc gccatcgctc tcatcacagg
     3841 acctacaggt cctcggcagc gcgctgacat tcgtcgaacc gcggccggca gggacgctgg
     3901 ccgtcgtcta cagcgccgct gatgaggcgt cccggcagga tgcggcggcg atcatgacag
     3961 cgctcgggaa gggcctgccg attggtggcg caatcctcac tccgacgctt gtcgatgcag
     4021 ccgcgcttgg ctcgaccgat ttcggcctcg ccatcatcgc agcgaacgcc aatggcgagg
     4081 ccgtggcccg gtccgttgat gctcggcatg cgctctgcgt caccgcggat cggcccgcgg
     4141 tgcaacgcgg gctctgcact gtcgccatca actcggccgg gcgggtggac atcttcctga
     4201 acaatcgcgt cgcgcagcgc gccggaatca cgtttgccac ggcgttccga atgatggtgc
     4261 acgagcaatg aaatactggc tttcatgcgt ggctctgtgc gtcacgatca tcgtggtggc
     4321 ggcgctatcg gtcagccccc gcgcacagca gcttgattac ggaacgctcg agaaggtgtt
     4381 ccacgaaccg gtcaccacat ctgccacggg ccagccgcag cgtgcgagcg aggcgccggt
     4441 aaacatgacg atcatcaccc aggacgatat ccgccgttcc ggcgcgacca ccatcccgga
     4501 cgcgctgcaa tacgtcgccg gtgtcgatgt gcgacgctac ggaatcgcgg acgaggaggt
     4561 tggcatccgc ggctacaacg cggcctacaa tccgcgcctc ctggtgttgg tgaacgggag
     4621 acaggtctat tcggacgatt acggccgcgt gttctggcca accgttc
//
LOCUS       DATGEM010000233         8925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_365025, whole genome shotgun sequence.
ACCESSION   DATGEM010000233 DATGEM010000000
VERSION     DATGEM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365025"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..543)
                     /locus_tag="VKQ27_07370"
     CDS             complement(205..543)
                     /locus_tag="VKQ27_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLY88789.1"
                     /translation="MKKIEAVIKPFKLDEVKEALHEVGLQGITVVEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVCDDAVLERAVEAIINSARTGRIGDGKIFVSPVEDVIRI
                     RTGERGEDAI"
     gene            835..2013
                     /locus_tag="VKQ27_07375"
     CDS             835..2013
                     /locus_tag="VKQ27_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HLY88790.1"
                     /translation="MKPANTLLSGIGTTIFTVMSALAVEHGSINLGQGFPDTEGPADV
                     VRAAADALLDGRNQYPPMPGVPELRQAVAAANRRFYGLEVDWASEVVVTSGATEAITA
                     CLMAVLNPGDEVVLIEPLYDTYLPVVRMLGCTPRLVRLEPPDWTLPRAELADAFGPKT
                     KAILLNSPMNPASKVFTEEELGFIAELVLRHDAYAICDEVYEHLVFDGERHIPLMTLP
                     GMRERCMRVGSAGKTFSLTGWKIGYVTTCPALAGNVAKAHQNLTFTTPPNLQRAVAMG
                     LAKDDAYFASLSGALQAKRDRLAAGLASLGLTVLPAKGSYFISTDFRPLGFNDDDVAF
                     CRHITEHAKVTAIPVSAFYDGPDAPSHYARFAFCKRDEVLDEAVARLRAHFGGRAAPG
                     "
     gene            complement(2016..2396)
                     /locus_tag="VKQ27_07380"
     CDS             complement(2016..2396)
                     /locus_tag="VKQ27_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88791.1"
                     /translation="MTLNPRPVLETDVPRTTFIDHVSPALPVDWAAGVGTFDVPLAAN
                     GATVLNVTSYRQISLLVGTTKATRAALFMGKISGSTLSSRIEFNLDGTIHTFPVVGPE
                     ISLELYANRSDPIEKVQLWLYLTS"
     gene            2552..2627
                     /locus_tag="VKQ27_07385"
     tRNA            2552..2627
                     /locus_tag="VKQ27_07385"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2585..2587,aa:His,seq:gtg)
     gene            2644..3225
                     /gene="mobA"
                     /locus_tag="VKQ27_07390"
     CDS             2644..3225
                     /gene="mobA"
                     /locus_tag="VKQ27_07390"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HLY88792.1"
                     /translation="MTGTAALILAGGRATRMGGGDKPLLAVAGRPMIARIIAALETRQ
                     VAISASGDPARFSVFGLPVLADGAFAGEGPLAGVLAGLDWAAGLGADTLLTVPGDTPF
                     IPLGLAGALTPAPACAASNGQLHPLVALWPVGCRDELRRLLSVPGRRDVAHFARSIGM
                     RRVDFPVAKWDAFMNVNTPEDLTVARTIAEEET"
     gene            3222..4025
                     /locus_tag="VKQ27_07395"
     CDS             3222..4025
                     /locus_tag="VKQ27_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HLY88793.1"
                     /translation="MTKTVAIGGLGAIGLKLARALDAGVDGLELIAVAARDRAKAEAN
                     LAGFRAPPQVVSLAELAQADVVVEAAPAAVFEQIATAAIDAGRVFVPASVGALLPRMH
                     LVQRAKGTGARIVVPTGALLGLDAVRAAAEGSVESVTIESRKPPRGLEGAPYLVKHGI
                     DVSGIVAPQRVFEGNAFDAAAGFPANVNVAAALALAGIGPVRTRVEIWADPGIDRNMH
                     TIRVEAEAARFSMTIENVPSAENPRTGQITALSMLACLRGLVSTLKVGT"
     gene            4057..5298
                     /locus_tag="VKQ27_07400"
     CDS             4057..5298
                     /locus_tag="VKQ27_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88794.1"
                     /translation="MQRLILAIAAFVLAIGGGAVRAADDVIIGVLYPMTGPNAVVGAD
                     AKAAMETAADIINGNHNIAHLILGKGGGLPNLGGAHVKLIIADHQGDPQKARAEAERL
                     ITQEHAVALIGSYQSATAATISQVADRYQVPYMSADNSSPSLNQRGLKWFFRTSPHDV
                     MFTQAMFDFFSAIGEKTGHKVKSVALIHEDTIFGTDSSNAQKQMAQAAGIKVVADIRY
                     RGNSPSMSAEVQELKAADADVIMPSSYTSDAILMLRGMHDVGYTPKAIMAQSAGFAEQ
                     AFIDAVGPLAEGVMSRSAFALDAAKARPAIPAVNALYRARNNKDLNDNTGRELIGLLV
                     LADAINRAGSVDEDKIHAALVATNIPGDETIMPWKGIKFDETGQNVEATPVIQQIKDG
                     KYHTIYPFDVASEPAVWNLGK"
     gene            5339..6214
                     /locus_tag="VKQ27_07405"
     CDS             5339..6214
                     /locus_tag="VKQ27_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88795.1"
                     /translation="MTGAILLQVAVGGLLMGLIYALVAAGLSLIFGLMDVVNFAHGEF
                     LMLAMYATFVLWLFTGLDPLLAVFPVALVLFGLGVAIYRLLMARALSVRFNRGMVQIF
                     VTFGLAIFLRGAAQFVFGSDFKSVSRSWLANRTVDLAGIYIPLPQLAAGLVSLVAFGA
                     LLAINRTEFGRALEATREDRDAVALIGIDRDRIFAFGWGLGSAAVGVAGAMLATFYYV
                     SPTVGANFALIAYVTVALGGFGSMTGALVAGLLIGEVEALTALLVEPSLKQVGMFVIY
                     LAVLMFRPRGLFGTL"
     gene            6342..7352
                     /locus_tag="VKQ27_07410"
     CDS             6342..7352
                     /locus_tag="VKQ27_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY88796.1"
                     /translation="MQTSITRRRRTELIVGGVLLVVLAVLPLPIHDVYTRNLIIITIM
                     FAGLAQAWNILGGYCGQISLSHALYFGAGAYVSTLLMLRLGVPPTVGMFAGGALGGLL
                     ALLVGWPCFRLSGHYYAIATLVVGEIGYLLVLNWDWVGGATGINIPYVRAHGDSWLWL
                     QFRIAKLPYHYVALAFASVAWLVAWIIEGSRWGFVWRAVKDDPIAARSLAVRVFPSKM
                     AAAAISGALTGIGGAIYAQYVGYIDPDSVLYGQLSILISLPAVLGGVGTLWGPLVGAA
                     VLIPMSELTRSYLGGSGSGVDLMIYGALIMLVALARPQGLASLIGVRVRAPAGADDAA
                     AA"
     gene            7336..8064
                     /locus_tag="VKQ27_07415"
     CDS             7336..8064
                     /locus_tag="VKQ27_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88797.1"
                     /translation="MPPLLEVDGVSKSFGGVAANVEISFDVVEGEILGLIGPNGAGKT
                     SLFNSISGEVEPDAGEVRLNGRRISGLRPEQCSLLGIARTFQVVRSFDSMTVLENVMV
                     GAFSRLSRARVAMARAMETLAFTGLAARADVAAHSLTPAEKRRLEVARALATEPKLLL
                     LDEMLTGLTPVEAQSGVQLIRDVRARGITVIMVEHVMEVLLPLVDRAVVLDLGRVLTI
                     GAPGEVVRHPEVIRAYLGDRYAAG"
     gene            8051..8749
                     /locus_tag="VKQ27_07420"
     CDS             8051..8749
                     /locus_tag="VKQ27_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88798.1"
                     /translation="MLRVEGVSASYRGLRALQGVSLEVAQGEIVAVVGANGAGKSTLL
                     KSIAGQVATEGVIEFEGVSLRRMAAHRITRLGVSMVPEGRRLFPRLSVEDNLRLGAYS
                     KRGADRFKPLELVFSLFPRLQERLPQRAETLSGGEQQMLAIGRALMTQPRFLMLDEPS
                     QGIMPRLVDDILAAVGRIRDLGVTVLLVEQRLVEALAIADRAYVLQTGRIVMEGPAAA
                     IAGDEAVRRAYLGI"
BASE COUNT         1369 a         2494 c         3329 g         1733 t
ORIGIN      
        1 tccacggaat gttccgtcag catctccagc actttcccga cgccgctggc cgcgcctgca
       61 ggctttctcg ccatgtatga ttaccccgtc gctagtcaca attggtgcgg gccgcaccat
      121 gcgaccgcat cccgtggcgg ccccctctac tggtttccgc caggggccga aagcggaatt
      181 cgtgcggttt tctgtcgttg cgcgctagat cgcgtcctcg ccacgttcgc cggtccggat
      241 gcggatgaca tcctcgaccg gcgagacgaa gatcttgccg tcgccaatgc gtccggtgcg
      301 ggcggagttg atgattgcct ccaccgcacg ctccaggacc gcgtcgtcac agacgacctc
      361 gatcttcacc ttggggagga aatcgaccac gtactcggcg ccacggtaca gctcggtatg
      421 acctttctgg cggccaaatc cctttgcctc gaccacggtg atgccttgca ggccgacttc
      481 gtgcagcgct tctttcacct catccagttt gaacggcttg atgacagcct cgatcttctt
      541 catgcgcgct cacatcccgg ctgcccgggc ctccctgtct ggctgcccgt gtcatacctg
      601 gacagaacgg cagcggcaca ggtttgcatg cggcgtgcca agcggcaatg caataagggt
      661 aaagggtcct gtatcgcggg agattgcgcc gcgacagtgc atatgacgcc ttgcttacgg
      721 cccggtcgcc aaatgcccag aatttgggca ctaaggcgcc acacctgttg ccgcagttga
      781 aggccgatgg tgctgaactc ataatcaaac cgcgtccaag ggagcctctc cgcagtgaag
      841 cctgccaaca cactcctgtc cgggatcggc accacgatct tcacggtgat gtcggcgctg
      901 gctgtcgagc atggctcgat caacctgggc caggggttcc cggacaccga ggggccggcg
      961 gacgtggtgc gcgcggcggc ggacgcgctg ctggatgggc gcaaccagta tccgccgatg
     1021 cctggcgtgc cggagctgcg tcaggccgtg gctgcggcga accggcgctt ctatgggctg
     1081 gaggtcgatt gggcgagcga ggtcgtggtg acgtcgggtg cgaccgaggc gatcaccgcc
     1141 tgcctgatgg cggtgctgaa cccaggcgac gaggtggtgc tgatcgagcc gctgtacgac
     1201 acatatctgc cggtggtgcg gatgctgggc tgcacgccgc gcctggtgcg gctggagccg
     1261 ccggactgga ccttgccgcg ggccgagctg gccgacgcgt tcggcccgaa gaccaaggct
     1321 atcctgctga attctccgat gaatccggcg agcaaggtgt tcaccgagga agagcttggc
     1381 ttcatcgccg agctcgtgct gcggcacgac gcgtatgcga tctgcgacga ggtgtacgag
     1441 cacctcgtgt tcgacggtga gcggcacatc ccgctgatga cgctgccggg catgcgcgag
     1501 cggtgcatgc gggttggcag cgcgggcaag acgttctcgt tgaccggctg gaagataggt
     1561 tatgtgacca cctgcccggc gctggcaggc aacgtggcga aggcgcacca gaacctgacc
     1621 ttcacgacgc cgcccaatct gcagcgggca gtggccatgg ggctggcgaa ggacgacgcc
     1681 tatttcgcct cactgtcggg ggcgctgcag gcgaagcgcg accgtctggc ggcggggctg
     1741 gcgagcctgg ggctgacggt gctgccggcg aagggcagct acttcatctc caccgatttc
     1801 cgcccgctgg gattcaacga cgacgacgtg gcgttctgcc ggcatatcac cgagcatgcg
     1861 aaggtgacgg cgatcccggt ctccgccttc tatgatggtc cggatgcgcc gagccattac
     1921 gcacgctttg cattctgcaa gcgcgatgag gtgctggatg aggcggtggc gcggctgcgg
     1981 gcgcatttcg gtggtcgcgc cgcgccgggc tgagcctacg aggtaaggta cagccagagc
     2041 tgcaccttct cgattgggtc ggacctgtta gcatagagct ccagcgagat ttccggaccg
     2101 acgacgggga atgtgtggat ggtaccgtca aggttgaact cgatcctgga ggagagcgtc
     2161 gacccgctta ttttacccat gaatagggcg gccctggtcg ctttggtggt gccgaccagc
     2221 aggctgatct ggcgatagct tgtgacattg agaacggtcg caccatttgc cgccaacggc
     2281 acgtcgaacg tcccgacgcc tgcggcccag tcgaccggca gcgcgggcga tacgtggtca
     2341 atgaaggtgg tcctggggac gtcggtttcc aataccggcc tgggattgag tgtcatggcc
     2401 gactccattt tgatgttgtg cgaagtgggc tggtggccca ggactacatg cttccctggc
     2461 ttcagcgatg tcattgaccc aggtcaaaat tgccgatcgt gcgcgagttg acggcaaccg
     2521 gcggaacgga ataaagccgg ccccccggat ggcggacata gctcagttgg tagagcgcca
     2581 ggttgtggtc ctggatgtca cgggttcgag tcccgttgtt cgccccaatt ccggcatttg
     2641 gcgatgaccg gtactgccgc actgatcctg gcgggtggcc gggcgacgcg catgggcggc
     2701 ggcgacaagc cgctgctcgc ggttgctggc cggccgatga tcgcgcggat cattgccgca
     2761 ctcgagacgc ggcaggtggc gatcagtgcc agtggcgatc cggcgcggtt ctccgtgttc
     2821 gggctgcctg tactggcaga cggggcgttc gcaggtgagg ggccgttggc cggtgtgctg
     2881 gccggcctcg attgggcggc cggcctcgga gccgatacgc tgctgacggt gcctggcgat
     2941 acgccgttca ttccgctggg gctcgccggg gcactgacgc ccgcgccggc ctgtgcggcg
     3001 agcaatggcc agctacatcc gcttgtcgcg ctctggccag tcgggtgccg ggatgaattg
     3061 cggcgactgt tgtcggtgcc cgggcggcgt gatgtcgcgc actttgcccg ctccatcggc
     3121 atgcggcggg ttgattttcc cgtggcgaaa tgggacgcgt tcatgaacgt caatacgccg
     3181 gaagatctga ccgtggcgcg aaccatcgct gaagaggaga catgacgaag actgtagcga
     3241 tcggcggtct cggcgcgatc gggctgaagc tggcgcgcgc cttggacgca ggcgtcgatg
     3301 ggctggaact gattgccgtc gcggcgcggg atcgggccaa ggcggaggca aatctggccg
     3361 ggtttcgggc gccgccacag gtcgtttcgc tcgcagagtt ggcgcaggct gacgtcgtgg
     3421 tggaggcagc gccggccgca gtgttcgagc aaattgccac ggcagcgatc gatgcggggc
     3481 gggtgttcgt gccggcctcg gttggggcgt tgctgcctcg gatgcatctg gttcagcggg
     3541 cgaagggaac aggtgcgcgc atcgtcgtgc cgacgggcgc actgcttgga ttggatgcgg
     3601 tgcgggcggc ggcggaggga tcggtggaaa gcgtaaccat cgaaagccgg aagccgccgc
     3661 gcgggttgga aggcgcaccc tatctggtga agcacggtat cgatgtgagc gggattgtcg
     3721 cgccgcagcg cgtattcgaa ggcaatgcat tcgatgcggc ggcgggattt ccggcgaacg
     3781 tcaatgtcgc cgcggcgctt gcgctggcgg gcatcggtcc ggtgcgaacc cgcgtggaga
     3841 tctgggccga tccggggatc gatcgcaaca tgcatacgat ccgcgtggag gcagaggcgg
     3901 cacggttcag catgacgatc gagaatgtgc cgagcgcaga gaacccacgg actggccaga
     3961 tcaccgcgct gtcgatgctg gcgtgtctgc ggggtctggt gtcaacgctc aaggttggca
     4021 cgtgaactgc atcgttttga ggtgaggggg attctcatgc aacgtttgat tttggcgatc
     4081 gcggcattcg tgctggcgat cggcgggggc gctgtgcgtg cggcagacga tgtgatcatc
     4141 ggcgtgctgt acccgatgac cgggccgaac gccgtggttg gtgccgacgc caaggctgcc
     4201 atggaaactg cagccgatat catcaatggg aatcacaaca ttgcgcatct gatactcggg
     4261 aagggcggcg gtctgcccaa tctcggcggg gcgcacgtca agctgattat tgcggatcat
     4321 cagggcgatc cgcagaaggc gcgggccgaa gcggagcgat tgatcacgca ggaacatgcg
     4381 gtggcgctga tcggatcgta tcagagcgcg acggcggcga cgatttcgca ggtggctgac
     4441 cgctatcagg taccgtacat gtcggctgac aactcgtcgc cgagcctcaa tcagcgtggc
     4501 ctgaaatggt tcttccgcac gtcgccgcac gacgtcatgt tcactcaggc gatgttcgac
     4561 ttcttcagcg ccatcggcga gaagaccggc cacaaggtga aatcggtcgc gttgatccac
     4621 gaggacacga tcttcggcac cgacagttcg aatgcgcaga agcagatggc gcaggcggcg
     4681 ggcatcaagg tcgtggcgga tatccgctac cgaggcaatt cgccctcgat gtcagcggag
     4741 gtgcaggagc tcaaggcagc ggacgctgat gtgatcatgc cgtcttcgta tacatcggac
     4801 gcgatcctga tgctgcgggg catgcatgac gttggctaca cgccgaaggc gatcatggcg
     4861 cagtcggccg gtttcgcaga gcaggcgttc atcgatgcag tcgggccgct ggctgaaggt
     4921 gtgatgagcc gctcggcctt cgcgctggat gcggcgaagg cgcgtcccgc cattcccgcg
     4981 gtgaatgcgc tgtaccgggc gcgcaacaac aaggacctga acgacaatac ggggcgcgag
     5041 cttattgggc tgctggtgct ggccgatgcg atcaaccggg cgggatcggt cgatgaggac
     5101 aagatccacg cggcgctggt ggcaacgaac attccaggcg atgagacgat catgccgtgg
     5161 aagggcatca agttcgacga gactggccag aatgtcgaag cgacgccggt gatccagcag
     5221 atcaaggacg gcaagtatca cacgatctat ccgttcgatg tcgcctccga gccggcggtc
     5281 tggaacctgg gcaagtgaca cgccgcctcg gcgcctgttc cggcgggcat gacgacgagt
     5341 gacaggggct atcctgcttc aggttgccgt cggcggattg ctgatgggcc tgatctatgc
     5401 gctcgtcgcg gccggcctgt cgctgatctt cgggctgatg gacgtggtga acttcgcgca
     5461 tggcgagttc ctgatgctcg ccatgtacgc gacgttcgtg ttgtggctgt tcacgggact
     5521 ggacccgttg cttgccgtgt ttcccgtggc gctggtgctg ttcgggttgg gtgtggcgat
     5581 ctaccggctg ctgatggcgc gcgcactgtc ggtgcggttc aaccgcggca tggtgcagat
     5641 tttcgttacg tttggcctgg cgatcttcct gcggggggcg gcgcagttcg tcttcggcag
     5701 cgacttcaag agcgtgagcc ggtcatggtt ggccaatcga acggttgatc tggcgggcat
     5761 ttatattccg ctgccacagc ttgccgccgg cctggtcagc ctggtggcgt tcggtgcact
     5821 gctggctatc aaccgcaccg agtttggcag ggcgctggag gcgacgcgcg aggaccgtga
     5881 cgccgtggcg ctgatcggca tcgaccgcga ccggatcttc gcgttcggct gggggttggg
     5941 gtccgcggcg gttggtgttg cgggagcgat gctggccacg ttctattatg tgtcgccaac
     6001 cgttggcgcg aacttcgcac tgatcgccta tgtgaccgtg gcgctgggcg ggtttggcag
     6061 catgaccggc gcgctggtgg ctggtctgct gattggcgag gtcgaggcgc tgaccgcact
     6121 gctggttgaa ccctccttga agcaggttgg catgttcgtg atctacctgg cggttctgat
     6181 gttccggccg cgcggactgt tcgggacgct gtgatggaac tagcgagtgc gactgccggg
     6241 actttctgtg gcgtggcgaa tgaagacgtg ggtgccgcgg ccaagccccg gcatgacgtc
     6301 gagggcggtg cgggggccca ggctcgacgg gtggcgccgt gatgcagaca tcgatcaccc
     6361 gacggcggcg gacggaactg atcgtcggcg gggtgctgct ggtggtgctc gcggtgctgc
     6421 cgctgccgat acatgatgtg tatactcgga atctgatcat catcaccatc atgttcgcgg
     6481 gcctggcgca ggcgtggaat atcctgggcg gttattgcgg gcagatctcg ctgagccacg
     6541 cgctgtattt cggtgccggg gcctatgtgt ccacactgct gatgcttcga ctgggcgtgc
     6601 ctccgactgt cgggatgttc gccgggggag cgctcggtgg gctgctggcg ctgctggtcg
     6661 gctggccgtg cttccggctc tcgggccatt actacgccat cgcgaccctg gtggtgggcg
     6721 agatcggtta tctgctggtg ctgaactggg actgggtggg tggtgccacc ggcatcaaca
     6781 tcccctatgt gcgcgcgcat ggtgattcct ggttgtggct gcagttccgg atcgcgaagt
     6841 tgccgtatca ctatgtggcg ctggcgttcg cctcggtggc gtggctggtg gcgtggatca
     6901 ttgaaggctc gcgctggggg tttgtctggc gcgccgtgaa ggacgatccg atcgcggcac
     6961 gcagcctggc ggtgcgggtg ttcccctcga agatggcggc ggcggcgatc agcggggcac
     7021 tgaccggcat cggtggcgcg atctacgcgc agtatgtggg gtatatcgat ccggattccg
     7081 tgctgtatgg gcaattgtcc atattgatct cgttgcccgc ggtgctgggt ggcgtgggca
     7141 cgctgtgggg accgctggtg ggggcggcgg tgcttatccc tatgtcggag ctgacgcggt
     7201 cttatctggg cgggtcgggg agcggcgtcg atctgatgat ctatggcgcg ctgatcatgc
     7261 tggtggcgct ggcgcggccg cagggtctgg cgagcctgat cggcgtgcgg gtgcgcgccc
     7321 cggcgggagc ggacgatgcc gccgctgctt gaggtcgacg gcgtcagcaa gtcgttcggc
     7381 ggcgtggcgg ccaatgttga gatcagcttc gatgtcgtcg agggcgagat ccttgggttg
     7441 atcgggccga acggggcagg gaagacgtcg ctgttcaatt cgatctcggg cgaggtggag
     7501 ccggatgcgg gggaggtacg gctgaacgga cgccgcatct ccgggctgcg gccggagcag
     7561 tgctcgttgc tcgggattgc gcggaccttt caggtggtgc gcagctttga ttccatgacg
     7621 gtgctggaga acgtcatggt gggggcgttc tcgcggctgt cgcgcgccag ggtggcgatg
     7681 gcgcgggcga tggagacgct ggcgttcacc gggctggcgg cgcgggcgga tgtcgcggcg
     7741 cattcgctga cgccggcgga gaagcggcgg ctggaggtgg cgcgggcgct ggcgaccgaa
     7801 ccgaagctgt tgctgctgga cgagatgctg acaggcctca cgccggtgga ggcgcagtcg
     7861 ggcgtgcagt tgatccgcga cgtgcgggcg cggggcatca ccgtgatcat ggtggagcac
     7921 gtgatggagg tgctgctgcc tctggtggat cgcgcggtgg tgctggatct cgggcgcgtg
     7981 ctgacgatcg gggcgccggg cgaggtggtg cggcatcccg aggtgatccg ggcgtatctg
     8041 ggggatcggt atgctgcggg ttgagggggt gtcggcgtcg taccgggggc tgcgggcgct
     8101 gcaaggcgtg tcgctggagg tagcacaggg cgagatcgtc gcggtggtgg gcgccaacgg
     8161 ggcgggcaag agcacgctgc tgaagtcgat cgcggggcag gtggcgaccg agggggtgat
     8221 cgagttcgag ggcgtgtcgc tgcggcggat ggcggcgcat cggatcacgc ggctgggggt
     8281 gtcgatggtg ccggaggggc ggcggctgtt tcctcggctt tcggtggagg acaatctgcg
     8341 gctgggggct tactcgaagc gcggggcgga tcggttcaag ccgctggaac tggtgttctc
     8401 gctgtttccc cgattgcagg agcggctgcc gcagcgtgcg gagacgctgt cgggcggtga
     8461 gcaacagatg ctggcgatcg ggcgggcgct gatgacgcag ccccggtttc tgatgctgga
     8521 tgagccgagc caggggatca tgccgcggct ggtggacgac atcctggccg cggtgggacg
     8581 gatccgtgac ctgggggtga cggtgctgct ggttgagcag cggctggtgg aggcgctggc
     8641 gatcgccgat cgggcgtatg tgctgcagac gggtcggatc gtgatggaag ggccggcagc
     8701 ggcgatcgcg ggggacgagg cggtgcggcg ggcgtatctg gggatttagg gggcatgggg
     8761 tggcggcaga aaagacgtgg ccgccgggcc caactgcttt cgcaggtgcc ggacgatgca
     8821 tatcgacgcc cgcgcgagat tgctccgctt cgctcgcaat gacggccgga gtgctgcgct
     8881 tcagggcggg gcggtggcga tcgcggcggc agggtcgagc gggtt
//
LOCUS       DATGEM010000234         5744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_245737, whole genome shotgun sequence.
ACCESSION   DATGEM010000234 DATGEM010000000
VERSION     DATGEM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245737"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VKQ27_07425"
     CDS             complement(<1..1043)
                     /locus_tag="VKQ27_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HLY88799.1"
                     /translation="MNVEAKCPMGNTVRGNAVLFEMTNRLWWPNQLDLSVLHPNPPAG
                     DPMGKDFNYRQEFKSLDLAAVKADIFALMTTSQEWWPADFGHYGPLFIRMAWHAAGTY
                     RIGDGRGGAGTGQQRFAPTNSWPDNANLDKARILLWPIKQKYGRKLSWADLLILTGNC
                     ALESMGFQTVGFGGGRPDVWEPENDTYWGPEREWLGDERYSGERDLANPLAAVQMGLI
                     YVNPEGPNGNPDPLKAAVDIRETFARMAMDDEETVALIAGGHAFGKTHGAADPSQYVG
                     SAPEGAPIEMQGLGWANSYGSGNGDDTITSGIEVIWTQTPTQWSNNFFKNLFEHEWEL
                     TKSPAGAHQWTPK"
     gene            1336..2949
                     /locus_tag="VKQ27_07430"
     CDS             1336..2949
                     /locus_tag="VKQ27_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HLY88800.1"
                     /translation="MRGRDLIAGLSVAGLMLPEAVAYAGIAGLPAQRAIFAAIAGCLI
                     YACVGQSRFAIVSPTSSAAAILAATLAAMPGSLAIKAGFATIAVAFTGIMFTAAAAAR
                     LGGLTGFISRPVLRGFAFGLAITIILKQLPILLGVSVHAPDLLHLVAALIRTIPAWSV
                     PSLAVGSVALAALVGLRRLPAVPGALLVLGLGIAASTLFRLPQHGVATVGMIDTSLVW
                     PSLPVLGWSEMSRLAQFTLPLVLILFAESWGTIRALALRHGDSVDANRELGALGAANL
                     ASAIVQGMPVGAGFSAGSASEAAGATSRLTGVVAAAGLAILILWARPLIADLPEPVLA
                     AVVIAALTHALDPGPLVRLWRIGRDQYVALGAALGVLALGVLDGMMVAITLSLAALIQ
                     RLATPYVAELGRLGRGHDFVDLARHPDAIAAPGITIWRPGQPLFFANCDMMLSDIVRR
                     AKAAACRAIILSLEESFDLDSTALDALAECDRALHDAGIRLQLARVHDHVRDVLASGG
                     MSDLLARCSYSVDDAVAALKAMLMSGEAK"
     gene            2949..3566
                     /locus_tag="VKQ27_07435"
     CDS             2949..3566
                     /locus_tag="VKQ27_07435"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HLY88801.1"
                     /translation="MRHDIEPQLHPVSIGDLRPTQMTVGLREVNRKRAEWRARGERDG
                     PDFLGRHMVPVVQGPKGRFYLIDHHHLVRALHEEGVEHVLVSVIANLGKLEKALFWTF
                     MDNRNWLHPFDADGKRHAHTRLPKSIGEMDDDPYRSLAGALRGAGGCAKDATPYAEFL
                     WADFLRRRIARKRVEETFQSALVTALDLARTSEASYLPGWCGPDE"
     gene            complement(3635..4270)
                     /locus_tag="VKQ27_07440"
     CDS             complement(3635..4270)
                     /locus_tag="VKQ27_07440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY88802.1"
                     /translation="MSVPASPPICLIVPGLGNSGPGHWQSIWERERGDCRRVELGRWN
                     DPLRNLWISRLDQAVTAAPSPVVLVAHSLGCLAVAWWAALLGGSALGPVQGALLVAPP
                     DPDRSNVDPRIARFAPIPTKPLPFPAIVVASRTDPYASFARSRAMARNWLADLIDMGR
                     SGHLNAESRLGSWQNGQALLQTLLGGRLPRPAERPLPRSGPSRRLRPATDV"
     gene            complement(4463..>5744)
                     /locus_tag="VKQ27_07445"
     CDS             complement(4463..>5744)
                     /locus_tag="VKQ27_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HLY88803.1"
                     /translation="DGPHATLIERRAETARGIAYSTIHPEHVLNVRAANMSALPDDPG
                     HFLRWLERRAIGPHNGFVPRRAYGAYLDELLDEAVARGGSRLRVIRSDARDVELGAEG
                     ADVLLDDGTRIRCDRIALALGNLPPTAPPGLDLERLPKGVHVADPWSHSLTDGLQGGD
                     TVLVIGTGLTMIDIALLLDAERFDGRIIALSRRGLIPLTHADALPGTIRAERPSPGCL
                     ALLADIRSRSKAVDWRSAVDELRPFTQSIWRAMPVAERRRFLRHLRPWWDVHRHRIAP
                     HVAERIAIMRREGRLEVIAGRPIGVAPLGDRSARVQLRRRGEDRIETVDVARIVNGTG
                     PQGDLTRTREPLLINLVRRRLIRPDPLHIGIDVDQNSRAIAGDGSASDRLLVLGPMTR
                     GNFWEIVAVPDIRQQVWSVARYLSAAHWVEGEGL"
BASE COUNT          907 a         1811 c         1947 g         1079 t
ORIGIN      
        1 ttcttcggcg tccattggtg cgcgccggcc gggctcttgg tcagttccca ctcatgctcg
       61 aacaggttct tgaagaaatt gttgctccac tgcgtcggcg tctgggtcca gatgacctcg
      121 atcccgctgg tgatggtgtc atcaccgttg ccgctgccat agctgttggc ccagccgagg
      181 ccctgcatct cgatcggcgc gccctcgggc gcagatccga catattgcga agggtcggcc
      241 gcgccatggg tcttgccgaa ggcgtggccg ccggcgatca gcgcgacggt ctcctcatcg
      301 tccatcgcca tgcgtgcgaa ggtttcacgg atgtcgaccg ccgccttgag cggatcggga
      361 ttgccgttgg ggccctccgg attgacgtag atcagaccca tctgcacggc agccagcgga
      421 ttggcgagat cgcgttcgcc gctgtagcgc tcatcgccca gccattcgcg ctcggggccc
      481 caataggtgt cgttttccgg ttcccacacg tcggggcggc cgccgccaaa gccgacggtc
      541 tggaagccca tcgactccag cgcgcaattg ccggtgagga tcagcagatc ggcccaggac
      601 agcttgcggc catatttctg cttgatcggc cagaggagga tgcgtgcctt gtcgagattg
      661 gcgttgtccg gccagctgtt ggtcggcgcg aaacgctgct ggcccgtgcc cgcgccgccg
      721 cggccgtcgc cgatgcggta ggtgccggcg gcatgccagg ccatgcggat gaacagcggg
      781 ccataatggc caaaatcggc cggccaccac tcctgcgacg tcgtcatcag cgcgaagatg
      841 tcggctttca ccgccgccag atcgaggctc ttgaactctt gccgatagtt gaagtccttc
      901 cccatcgggt cgcccgccgg agggttcgga tggaggaccg acaggtccag ttggttcggc
      961 caccacaacc ggttggtcat ctcgaacaga actgcgttcc cgcgcaccgt gttgcccatc
     1021 gggcatttgg cttcgacgtt catgcctact cctctcgcag ttagcgttgc tcgacccgtg
     1081 ttccgggaaa catggcgtcc cggcgttcgg ctgtccaatc gcctaaattt ctctgactga
     1141 tcgaagttta ctttggaacg aggtgtccgc ccgggtggta tgcctctcga aaatgcggcg
     1201 tttacgtggt tcgagcttac cggagtccga tgacgctgac catggcgtgt ctggcaggcg
     1261 gacgttcgat caggccaacg ttccgtcgag cgccatcccc cttgccaaag gcgggttgcg
     1321 gcgagcaaat ggcgcatgcg gggacgcgat ctgattgcgg ggctatcggt tgcgggcctg
     1381 atgttgcctg aagcagtggc ctatgccggg atcgccggct tgccggcgca gcgcgccatc
     1441 tttgccgcga tcgctggttg tctgatctat gcctgcgtcg gacagagccg gttcgcgatc
     1501 gtttctccga cgtcgtctgc ggcggcgatc ctggccgcaa cacttgcggc gatgccagga
     1561 agcctcgcga tcaaggccgg gttcgcgacg atcgcggtgg cgttcacagg cattatgttc
     1621 accgccgcag ccgcagccag gctcggcggg ttgaccggtt tcatatcccg tcccgtgttg
     1681 cggggcttcg cattcgggct ggcgatcacc atcatcctga agcagctacc aatcctgctc
     1741 ggcgtatcgg ttcatgcgcc ggaccttctc catctggtcg cggccttgat ccgcacgata
     1801 cccgcctgga gtgttcctag ccttgccgta gggagcgttg ccctggctgc gctggtggga
     1861 ttgcggcgcc tgcctgcggt tcccggagcc ttgctcgtgc ttggattggg catcgcggct
     1921 tcgacgctgt ttcggcttcc tcaacatggg gtggcaaccg tcggcatgat cgacacgagc
     1981 cttgtctggc cttcgcttcc agtcctgggc tggtcggaga tgtcgcggct ggcgcagttt
     2041 accctgccgc tcgtcctgat cctgttcgcc gaatcctggg gaaccatccg tgcgctcgcg
     2101 ttgcggcatg gggattcggt ggatgcgaac cgcgagctcg gtgcgctggg ggccgccaac
     2161 ctcgccagcg cgatcgttca gggaatgcca gtgggggcgg gcttctcggc cggctcggcg
     2221 agcgaggctg caggggcgac gagccggctg accggagtgg tggccgcagc cggtcttgcc
     2281 atcctgatcc tttgggcgcg gccgctgatc gcagacctgc ccgagcccgt gctcgccgcc
     2341 gtggtgatcg cggcgctgac ccacgcgctg gatcccgggc cgctggtcag gctctggcgg
     2401 atcgggcgtg atcaatatgt cgcgcttggg gctgcgctgg gagtattggc gctgggcgtg
     2461 ctcgacggca tgatggtcgc gattacgctg tcgctggccg cactgatcca gcggctggcg
     2521 actccctatg ttgcagagct tggacgattg gggcgcggcc atgatttcgt ggacctggcg
     2581 cggcatcccg atgccatcgc ggcaccgggc atcacaatct ggcgtcctgg gcagccgctt
     2641 ttcttcgcca attgcgacat gatgttaagc gacattgtca ggcgcgcgaa ggcggcggcc
     2701 tgccgtgcga tcattctgag cctggaggag agtttcgatc tcgacagcac cgcgctcgat
     2761 gcgctggcgg aatgcgatcg agcgctgcat gatgccggta tccggctgca gctcgctcgc
     2821 gtccatgatc atgtccgcga tgtgctggcc agcggtggga tgagcgatct gctcgcgcgc
     2881 tgcagctaca gcgtcgacga tgctgtcgca gcactcaagg cgatgctcat gtccggggag
     2941 gccaagtgat gaggcatgat atcgaaccgc agctccatcc cgtgtcgatc ggcgatctgc
     3001 ggcccaccca gatgacggtc gggcttcgcg aggtgaaccg caagcgggcc gaatggcgcg
     3061 cacgcgggga gcgcgacggg cccgatttcc tgggccggca catggtgccg gtggtccagg
     3121 gccccaaggg gcggttctac ctgatcgatc accatcatct cgtccgcgcg ctgcatgagg
     3181 aaggggtcga gcatgtcctc gtgtccgtga tcgccaacct cggcaagctc gagaaggcgc
     3241 tgttctggac gttcatggac aatcgcaact ggctgcatcc gttcgatgcg gacggcaagc
     3301 gtcacgccca cactcgtctc ccgaagtcga tcggcgagat ggatgacgac ccctatcggt
     3361 cgctcgccgg cgctcttcgg ggcgccgggg gttgcgccaa ggatgccacg ccctatgcgg
     3421 aattcctctg ggcagatttc cttcggcgac ggatcgcccg caagagggtc gaagagacat
     3481 tccaatccgc gctggtcact gccctcgacc tcgcacgcac gtctgaggcc agttacctgc
     3541 ccggctggtg tggacccgac gaatgatcga tagatttacg tcatgctcct aaaatggctt
     3601 cgatcatttt cgtgagttca ctcctcgtcg attcctatac gtcggtcgct ggccgcaagc
     3661 gcctggacgg gcctgatcgc gggagcgggc gttctgctgg acgcggcaac cgcccgccca
     3721 gaagcgtctg gagcaacgcc tgcccgtttt gccatgagcc gagacgcgat tcggcgttca
     3781 gatgacctga tcgccccatg tcgatcaaat cggccagcca gttgcgcgcc atcgcgcgcg
     3841 agcgagcgaa gctcgcataa gggtcggtgc ggctggcaac gacgattgcc gggaagggga
     3901 gtggtttggt cggtatcggc gcgaagcggg cgatgcgtgg atccacgttc gaccggtcag
     3961 ggtcgggcgg tgcgacaagc agggcgccct ggacaggacc aagcgccgag ccgcccagaa
     4021 gcgccgccca ccaggcgacc gccaggcagc cgaggctgtg cgcgaccagc acgaccgggg
     4081 atggcgcagc ggtcacagcc tgatcgaggc ggctgatcca cagattgcgc aggggatcgt
     4141 tccagcggcc aagctcgacc cttctgcaat cgccacgctc ccgctcccag atgctttgcc
     4201 aatggcccgg ccctgaattg cccagtccgg gaacaatgag gcagatcggc gggcttgcgg
     4261 gaacggacat gacgcaggtc tccggctact gggctgcagc ctatcggccc gttggacgct
     4321 gtcttgtcag gaaaaatgtc tagtcgaata gtagggattt aatcgcagag ttcagaggcc
     4381 tgaaaacacg accctatcgc gtctggctgg accaacgcga tggctctctc ctttcggcca
     4441 tgtcggcgcc ggaccctctg actcagagac cttcgccttc gacccagtgc gcggcagaga
     4501 ggtaccgggc gaccgaccag acctgctggc ggatgtccgg tacggcgacg atctcccaga
     4561 agttgccgcg tgtcatcggg ccgagcacga ggaggcgatc cgatgcgctt ccgtcgcccg
     4621 caatcgcccg ggaattctgg tcgacgtcga tgccgatatg cagcggatcg gggcggatga
     4681 gcctgcgccg gacgagattg atgagcaggg gctcgcgcgt gcgggtaaga tcgccctgcg
     4741 ggccggttcc gttgacgatg cgcgcgacgt cgaccgtttc gatcctgtcc tcgccccgcc
     4801 gccgcagctg cacgcgtgcg gatcgatcgc cgagcggggc gacgccgatc gggcgtcctg
     4861 cgatcacctc gaggcgcccc tcgcggcgca tgatggcgat gcgctcggcg acatgggggg
     4921 cgatgcggtg acggtggacg tcccaccagg gcctcaaatg ccgcaggaaa cggcgacgct
     4981 cggccacggg cattgcgcgc cagatgctct gggtgaaggg ccgcaattca tcgacggcgg
     5041 accgccagtc caccgcttta gaccgcgacc gaatgtcggc cagcagtgca aggcagccgg
     5101 gggaaggccg ctcggcgcgg attgtcccgg gaagcgcgtc cgcatgggtc agcgggatca
     5161 gcccgcgccg ggacagggcg atgatgcggc cgtcgaaccg ctctgcgtcg aggagaagcg
     5221 cgatgtcgat catggttagc ccggtgccga tcacgagcac cgtatcgccc ccttgcagcc
     5281 cgtcggtgag cgagtgggac cagggatcgg cgacatgcac gcctttgggc aatcgctcca
     5341 ggtcgaggcc tggcggggcc gttggcggca ggttgccgag cgcaagcgcg atgcgatcgc
     5401 aacgaatgcg cgtgccgtcg tcgagcagga catcggcgcc ctcggcgcca agctccacgt
     5461 cgcgcgcgtc cgagcggatg acacgaaggc gcgatccacc tctggcaacc gcctcgtcga
     5521 gcaactcgtc gagataggca ccataagcgc ggcgtgggac gaagccgttg tgcggcccga
     5581 tcgcgcgccg ttcgagccag cgcaggaaat gcccggggtc gtcgggaagc gcgctcatgt
     5641 tggcggcgcg gacgttcaga acatgttcgg gatggatcgt cgaataggcg atgccccgcg
     5701 cggtttcggc gcgtcgttcg atcagcgtcg cgtgcgggcc gtca
//
LOCUS       DATGEM010000235         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1703910, whole genome shotgun sequence.
ACCESSION   DATGEM010000235 DATGEM010000000
VERSION     DATGEM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703910"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..492)
                     /locus_tag="VKQ27_07450"
                     /pseudo
     CDS             complement(1..492)
                     /locus_tag="VKQ27_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            765..2225
                     /gene="istA"
                     /locus_tag="VKQ27_07455"
     CDS             765..2225
                     /gene="istA"
                     /locus_tag="VKQ27_07455"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLY88804.1"
                     /translation="MRKIREVLRLKAERLSDRQIAAAIGSARSTVQECIRRCREAGLA
                     WPLAPELDEEALHARLYCRHVPLSRTPAPDFAKLHAELKRPGVTRMLLWQEYKATQPD
                     GWQYSEFCEQYRRWLGKQDAVLRQVHPPGDKLFVDYAGQTVPITDRLTGLVREAQIFV
                     AVLGASNYAFAEATWTQTLPDWLGSHVRALNFFGGVAAAIVPDNLKSGVHKSCRYEPA
                     LNPEYHDFAEHYDVAILPARVRKPRDKAKVEGGVLIVERWILARLRNCTFFSLAELNK
                     AIAALLGELNTRPFQRQEGNRLSLFERIERPALRPLPMKPYEFAAWKKAKVNLDYHVQ
                     IERAFYSVPYQLIGKTVDVRLTKHGIEIFHRQRLVAAHVRGAERGHFRTDATHRPERH
                     SAVIDLTHEKLMQRAVAIGPATVEVLAQQVQRRRHPEEALRASLGILRLAKDFSPDVL
                     ERAAERAVALGAFSYRAVRALIGAPSTVVARAKVTG"
     gene            2311..>2664
                     /locus_tag="VKQ27_07460"
     CDS             2311..>2664
                     /locus_tag="VKQ27_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLY88805.1"
                     /translation="MSLYAKIRRLYFREKTPISEIARRTSLSRNTVKKWLRAAERSEL
                     KYTRKPGPTKLDPHIEQLQQAMAADAHRPKRDRRTLKTLFRQLQAQGYRGSYSRLTQN
                     VRRWQAQHGAVTARAA"
BASE COUNT          492 a          847 c          847 g          478 t
ORIGIN      
        1 cacgccgcag ccagtcgcgc agcatgcggg cgccggccca cgggtaatcc aggtgcaact
       61 cgtcgattcg gcgcatcagc gccaagtctt ccgggctcac cggttgcggc aggtagtaca
      121 ccgtgccgcg gctgatgccg acgagttcgg cctgacgggt aatggacaac gtgtggctgc
      181 ggtcgatcaa cgctttgcgc tcagcagtcc cgccttggtg agcgcgcctc ctaaaaaatc
      241 gatctccagc gcctgctggc cgatcttcgc gtgcagtgcc ttcaaatcga ccgatggctc
      301 ctcggcgtgg tgcgcttgac cgaacacgct ggccgcctgt tcgagcaact gcgtcttcca
      361 ctgcgcgatt tgcgtggcat ggacaccgaa ctgctcgccc agttcggcga gcgtcttgtc
      421 gccgcgcacg gcggccaagg ccactttggc tttgaattcc ggtgaatggt ttcgacgggt
      481 tctgcgcgtc atgttctgct ccaagtagcg aggcgtatca tcgcctcatc gagcagaaat
      541 tccacctatc gcactgtcaa aatttccggg gccagctctc aaactgcgta ttccggtcca
      601 tgccgggcca cgattccgga gcatgccggg ccgggattcc ggtcgatgcc gggccaccat
      661 tccggtccat gccggcaggg gtgagtaaag cgggttaacc gaagctaccg tgcgctcttt
      721 ttccatctgg aaggagagtg acggtggcgc aatcgaggtt acccgtgcgc aaaatacgag
      781 aggtgttgcg gctcaaggcc gagcggctga gtgatcgcca gattgcggcg gcgatcggca
      841 gtgcgcgctc caccgtgcag gagtgcatcc gccgttgtcg cgaggccgga ctggcgtggc
      901 cgctcgcgcc ggaactcgac gaagaggcgt tgcacgcacg attgtattgt cgccacgtac
      961 cgctgtcgcg cacgccggcg ccggactttg ccaagctgca cgcggaactc aagcgtcccg
     1021 gcgtcacgcg catgctgctg tggcaggaat acaaggccac gcagccggac ggctggcagt
     1081 acagcgagtt ttgcgagcag taccgacgct ggctgggcaa acaggatgcc gtactgcggc
     1141 aagtccaccc gcccggcgac aagttgttcg tcgattacgc cggccagacc gtgccgatca
     1201 ccgatcggct caccgggctg gtgcgcgaag cgcagatttt cgtggcagtg ctcggcgcga
     1261 gcaattacgc cttcgcggaa gccacgtgga cgcagacgct gccggactgg ctgggatcgc
     1321 acgtacgcgc gctgaacttt ttcggcggcg tggcggcggc gatcgtgccg gacaacttga
     1381 agtcgggcgt gcacaagtcc tgccgctacg aaccggcgct caaccccgag taccacgact
     1441 tcgccgaaca ctacgacgtg gcgattctgc cggcgcgcgt gcgcaaaccg cgcgacaagg
     1501 ccaaggtcga aggcggcgtt ctgatcgtcg aacgctggat cctggcgcgc ttgcgcaatt
     1561 gcacgttctt ttcgctggcc gagctcaaca aggcgattgc cgcgttgctc ggggagctga
     1621 acacacggcc attccagcgc caggaaggca atcgcctctc gctgttcgag cgcatcgaac
     1681 gtccagcgtt gcggccgttg ccgatgaagc cgtacgagtt tgcagcctgg aagaaggcca
     1741 aggtgaacct cgattaccac gtgcagatcg agcgtgcgtt ctactccgtg ccgtatcaac
     1801 tgatcggcaa aaccgtcgac gtgcgcctca cgaagcacgg catcgagatt ttccatcgcc
     1861 aacgcctcgt cgccgcgcac gtgcgcggcg ctgagcgcgg tcatttccgc acggacgcta
     1921 cgcatcggcc tgagcggcac agcgccgtga tcgacctgac ccacgagaag ctgatgcaac
     1981 gcgctgtcgc gatcggcccg gcgaccgtgg aagtgctcgc acaacaagtg caacggcgcc
     2041 gacaccctga agaagccttg cgtgccagcc ttggcattct gcgcttggcc aaagattttt
     2101 cgcccgacgt gctcgaacgc gctgccgaac gcgcggtggc cctcggcgct ttcagctatc
     2161 gcgccgtgcg cgcgctgatc ggcgcaccat ccactgttgt ggcgcgtgca aaggtgaccg
     2221 gatagccgag gatagtttcg cctaaaactg atcagggtgt tcaacctatt ctgctgactt
     2281 ctgagttagc aggactacga ggtgatcacc atgagtctat acgccaagat ccggcggctg
     2341 tatttccggg agaagacccc gatcagcgag attgcacgac gcacgagtct gtcgcgcaac
     2401 acggtcaaga agtggctccg ggctgcggaa cgctcggaac tgaagtacac gcgcaagcca
     2461 gggcctacca agctcgaccc gcatatcgag caactgcagc aggccatggc ggccgatgcg
     2521 catcgcccca agcgcgatcg gcgcacgctg aagacgttgt ttcggcagct ccaggcgcag
     2581 gggtatcgcg gcagctattc gcgcctcacg cagaacgtgc gccgctggca ggcgcagcat
     2641 ggcgcggtga cggcccgcgc ggcc
//
LOCUS       DATGEM010000236         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_188150, whole genome shotgun sequence.
ACCESSION   DATGEM010000236 DATGEM010000000
VERSION     DATGEM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188150"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..540)
                     /locus_tag="VKQ27_07465"
     CDS             complement(22..540)
                     /locus_tag="VKQ27_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88806.1"
                     /translation="MNDTTTVALSPETVLAHMKQNRVTDVVWLPDSETNWLYMLMKAE
                     PSLRLVGVTREGHACSIAAGLHAGGRKPLILIQNTGMMESGDSIRGWLLGLNVPVVLM
                     VGYRGWTRHGVNTDTAATYTERFLMAFGLNYYLVESDADAERISIAFQEAERTQRPVV
                     VLVGDEYHGFNR"
     gene            624..1496
                     /locus_tag="VKQ27_07470"
     CDS             624..1496
                     /locus_tag="VKQ27_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY88807.1"
                     /translation="MANTFRAGNMTVHRIIEMECGFTPALEFLPKLKPEVLAENRSWL
                     APAALDAQDNLVLCFQSYIVQTGRHNILVDSCIGNDKDRTARPLWHKKKDDTFMKGLA
                     AAGLTVNDIDFVMCTHLHVDHVGWNTRLENGRWVPTFPKARYLFSKKELDYWLAENAK
                     TELPPIVDSVIPIVEAKRCDLVTSDYAMDDTIALLPTPGHTIDHYAVAMGRGGKDAVI
                     TGDLIHSPLQAKYPELGMRVDYDTEQGADTRRKFLETYCDTDTLCCFAHFPSPSRGHI
                     KRWGTGFRCEYVND"
     gene            1570..2439
                     /locus_tag="VKQ27_07475"
     CDS             1570..2439
                     /locus_tag="VKQ27_07475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HLY88808.1"
                     /translation="MKFGLRYCNTGRYTDPARAVELMQAAEAAGFESAWTVEHTVIPR
                     GYQSAYPYTADGKLPGGEGDFLLPDPLIWMAYVAAATTRIKLATGILILPQHNPVIVA
                     KQVATLDHMSNGRILLGIGVGWLKEEFDAVGASFHDRGRRTDEYIAVMRELWSADAPN
                     FSGEYVNFRDAFMRPKPVKRNVPIIIGGQTAPAARRAGRLADGFFPGRGLPIDLFETV
                     RRAARDAGRDPAAVELTVGIPEDMSKLADYAAAGVSRVLIPTTGVAGLARKIAGPGDL
                     LAWRETIERFASL"
     gene            2436..3071
                     /locus_tag="VKQ27_07480"
     CDS             2436..3071
                     /locus_tag="VKQ27_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HLY88809.1"
                     /translation="MTLLIDLDGTLIDSRPGIAASLEAALRALGHTPDPAFDITPMIG
                     PPLPSVIGRVLALYNDDRVDIGIDAYRAHYGDVGLHQFTLYPGIAETLPALAEHQRCF
                     VVTSKRSLYAKRIIEKLGYTTMLGGVYGTEADGSLDDKADLIAAVLHKEDLDPSETIM
                     VGDRLHDIHGAHANGLRAVGVLWGYGSRAELEQAGADTLVSGPAELLGLVA"
BASE COUNT          592 a          951 c          987 g          612 t
ORIGIN      
        1 ctcgcaatga catgggggca ctcatcgatt gaatccgtga tattcatcgc cgaccagcac
       61 aacgactggc cgttgcgttc gctcggcttc ctggaacgcg atggaaatgc gctctgcgtc
      121 ggcatcactt tccaccaggt agtagttgag gccgaacgcc atcaggaagc gttccgtata
      181 ggttgccgca gtgtcggtgt ttacgccgtg gcgagtccag ccgcgataac ccaccatcag
      241 caccacgggc acattcagcc ccagaagcca gccgcggatg gagtcacctg actccatcat
      301 cccggtgttc tggatcagga tgagcggctt gcggccaccg gcatgcaatc ctgccgcgat
      361 cgagcaggcg tgtccctcgc gcgtcacgcc gaccagccgc aaggacggtt cggctttcat
      421 cagcatatac agccagttgg tttcgctgtc cggaagccag acgacgtcgg tgacgcggtt
      481 ctgcttcatg tgagcaagca ccgtctcggg gctgagggcc accgtcgtcg tatcgttcat
      541 cgtccactcc cagcgtgaga tttgtcggca ggcggggcag tagctaacat cataccgata
      601 aaccaccgca agggagcgat ccaatggcca acaccttccg cgccgggaac atgaccgttc
      661 accgcataat cgagatggaa tgcggcttta cgcctgccct ggagttcctg ccaaagctga
      721 agcctgaggt gctggcggag aaccgctcgt ggctggcacc ggcggcactg gatgcccagg
      781 acaacctggt gttgtgcttc cagtcctaca tcgtgcaaac cggccgtcat aatatcctag
      841 ttgatagttg catcggcaat gacaaggatc gcacggcgcg gccgctgtgg cataagaaga
      901 aggacgatac attcatgaag ggcctggccg cggcaggcct gaccgtcaac gacatcgact
      961 tcgtcatgtg cacccatctc cacgtcgatc atgtcgggtg gaatacgagg ctcgagaacg
     1021 ggcgctgggt cccgaccttc cccaaggcac gatatctgtt ctccaagaag gagctcgact
     1081 attggctggc tgagaatgcc aagaccgagc tgccaccgat tgtggacagc gtgattccga
     1141 tcgtcgaggc gaagcgttgt gacctggtga ccagcgacta cgccatggac gatacgattg
     1201 ccctgctgcc cacgccgggc cacaccatcg accactacgc ggtggcaatg ggccgcggcg
     1261 gcaaggacgc ggtgatcacc ggcgatctga tccactcacc attgcaggcg aagtatcccg
     1321 aacttgggat gcgcgttgac tacgacaccg aacagggggc cgacacacgc cgcaagttcc
     1381 tggagacgta ttgcgacacc gacacgctgt gctgcttcgc gcacttccct tcgccgtccc
     1441 gtgggcacat caagcgctgg ggcacagggt tccgctgcga atacgtcaac gattgaggct
     1501 gcgtgttcct gcttcatcgg catcatggca gcgacacgta gccatgatgc cgggtggatg
     1561 gactcgaaaa tgaaattcgg tctgcgttat tgcaatactg gccgctacac tgatccggcg
     1621 cgtgccgtcg aactgatgca ggccgctgaa gccgcgggct tcgagtccgc atggacggtg
     1681 gagcacactg tcattccgcg cggctaccag tctgcgtatc cctacacagc ggacggcaag
     1741 ctcccgggcg gcgagggcga tttcctgttg cctgatccgc ttatctggat ggcctatgtc
     1801 gctgccgcga ccacgcgcat caagcttgcc accggcatcc tgatcctgcc gcagcacaat
     1861 ccggtcattg tcgccaagca ggtcgcgacg ctggaccaca tgtcgaacgg acgcatcctg
     1921 ctcggcatcg gtgtcggctg gctgaaggaa gagttcgatg ctgtcggcgc cagcttccat
     1981 gatcgtggcc ggcgtactga cgaatatatc gccgtcatgc gcgaactttg gtcggctgac
     2041 gcgccgaatt tttccggcga atatgtgaac ttccgcgatg cgttcatgcg gcccaagccg
     2101 gtgaagcgga acgtgccgat cataattggc ggccagaccg caccggcggc gcgtcgtgcg
     2161 ggacggcttg ccgatgggtt ctttccgggg cgcggcctgc cgattgatct gtttgagacc
     2221 gtgcgccggg cggcgcgcga tgcggggcgc gatccagctg cggtggagct tacggtgggt
     2281 atcccagagg atatgtcgaa gcttgctgac tatgccgcgg cgggcgtgtc gcgtgtactg
     2341 ataccaacca ctggcgtcgc tggcctggcg cgcaagatcg ccggcccggg ggacttgctt
     2401 gcctggcgtg aaacgatcga gcgtttcgcc agcctgtgac cctgctgatc gatctcgacg
     2461 gcacgctgat cgactcgcgg cccggaatag cggcgagcct ggaagcggcg ctgcgtgcgc
     2521 tgggccacac gccggatccg gcgttcgaca tcacaccgat gatcggcccg ccgctgccct
     2581 cggtgatcgg ccgcgtgctg gcgctctata atgacgatcg tgttgatatc ggtatagacg
     2641 cctatcgcgc acattacgga gatgtgggtc tgcaccagtt cacgctgtat ccaggtattg
     2701 ccgagacgct gcctgcgctg gcggagcatc agcgttgctt cgtcgtcacg tccaagcgca
     2761 gcttgtatgc gaaacgtata atcgagaagc tgggctatac gaccatgctc ggcggtgtct
     2821 atggcactga ggcggatggc agcctggacg acaaggccga tctgattgcc gcagtcctgc
     2881 acaaggaaga tctcgatccg agcgagacga tcatggttgg cgatcgcctg cacgatatcc
     2941 atggcgccca tgcgaacgga ttgcgtgccg tcggcgtgtt gtgggggtat gggagccgcg
     3001 ccgaactaga gcaggcagga gccgatacgc tggtgagcgg accggctgag ctcctggggc
     3061 ttgtcgcctg aaccggcccc gcgtgtatac aggatcactc gtcggcggcg gccttgcgcg
     3121 ccatagcctc ttccgcgctg ga
//
LOCUS       DATGEM010000237         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_706714, whole genome shotgun sequence.
ACCESSION   DATGEM010000237 DATGEM010000000
VERSION     DATGEM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706714"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VKQ27_07485"
     CDS             complement(<1..558)
                     /locus_tag="VKQ27_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HLY88810.1"
                     /translation="MNTIDSPLLGLSQRLPPSNLQAEQALLGALLANNRAYERVSEFL
                     SPEHFADPIHGRIYQAIVRRIEAGQLADAVTLKAEFEHSGILEEVGGTAYLAQLLTAM
                     VGIINAGEYGRAIHDAWLRRQLIDIGETVVNNAFGADAELDGAQQIEAAEHTLFQLAT
                     DGSVSGGFISFERALTEAIVGAERAF"
     gene            complement(637..1878)
                     /locus_tag="VKQ27_07490"
     CDS             complement(637..1878)
                     /locus_tag="VKQ27_07490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HLY88811.1"
                     /translation="MQAVLDAITRRFIKFGSLMVRWPDGRTTTYTGPEGSGPEASFAL
                     RDNAAIRRMVLNPTLAVGECYMDGGLIPDERGIYNVLDLLMLNLESSSQGHPIARIRS
                     ALGLLKRRLDQYNPATRAQRNVAHHYDLNGRLYSLFLDRDRQYSCAYFPRGDETLEQA
                     QIAKKRHIAAKLCLDRPDLTVLDIGSGWGGLALTLARDYGARVTGITLSTEQLNESQA
                     RAAAEGLTDRVSFELLDYRAMDRKFDRIVSVGMFEHVGVGFYRAFFDTIARCLKPDGL
                     ALLHAIGRSDGPGFTNPWIAKYIFPGGYCPALSEVLPPLEQSRLVATDVEILRLHYAE
                     TLRHWRRRFTANRDTIAAIYDERFCRMFEFYLCGSEIAFRRAGQMVMQIQLAHQQTAA
                     PLTRDYITDRERSVARTKADA"
     gene            complement(1956..>2324)
                     /locus_tag="VKQ27_07495"
     CDS             complement(1956..>2324)
                     /locus_tag="VKQ27_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494969.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLY88812.1"
                     /translation="GVSPDQVQYVNAHGTSTPLGDDLELEAVERVFGAAAKGLAMSST
                     KSATGHLLGAAGAVEAIFSILAVRDNVAPPTLNLEEPSRESVIDRVARAAQDRRIDHA
                     LSNSFGFGGTNASVIFRSVN"
BASE COUNT          421 a          691 c          788 g          424 t
ORIGIN      
        1 aaacgcgcgt tcagcgccga cgatggcttc ggtgagagcg cgttcaaagc tgatgaagcc
       61 gccactcacc gagccgtccg tggcgagctg gaacagtgtg tgttccgctg cttcgatctg
      121 ctgagcgccg tcgagttccg catcggcgcc gaacgcgttg ttcaccacgg tctcgccgat
      181 atcgatcagc tggcggcgta gccacgcatc gtggatcgca cggccatatt cgccggcatt
      241 gatgatgccg accatcgcgg tgagaagctg cgccaggtac gcggtaccgc cgacttcctc
      301 gaggatgccg gagtgctcga actccgcctt cagggtgacg gcgtcggcaa gctggcccgc
      361 ttcgatgcgg cgcacaatgg cctgatagat gcggccgtgg atcgggtcgg cgaagtgctc
      421 tggggacagg aattccgaga cgcgctcata ggcccggttg tttgccagca atgctccgag
      481 cagcgcctgt tctgcctgca ggttggatgg cggcaggcgc tgggaaagac cgagaagggg
      541 cgaatcgatg gtgttcactg cctgcgagca taggcgcctg cgcggaggaa cggcagtggt
      601 cgcattacgg ggataacgtg gatatcgggg actaaactaa gcgtccgctt tcgtcctggc
      661 cacgctgcgt tcgcgatccg tgatgtagtc gcgtgtcagc ggcgcggccg tttgctgatg
      721 ggcgagctgg atctgcatca ccatctgccc ggcgcgacgg aacgcgattt ccgagccgca
      781 caggtagaat tcgaacatgc ggcagaagcg ttcgtcgtaa atcgccgcga tcgtgtcgcg
      841 attggcggtg aagcgccggc gccagtggcg taatgtctcg gcgtagtgca gtcgcaggat
      901 ctcgacgtcg gtggcgacca ggcggctctg ctccagtggc ggcagcactt cggacagggc
      961 agggcagtag ccgccgggga agatgtattt ggcgatccag ggattggtga agcctggacc
     1021 gtcgctgcga ccgatcgcgt gcagcagcgc cagaccgtcg ggcttgaggc atcgcgcgat
     1081 cgtgtcgaag aaggcgcgat agaagcccac gccgacatgt tcgaacatgc cgacggatac
     1141 aatgcgatcg aacttgcgat ccattgcgcg gtaatcgagc aactcgaatg acacgcgatc
     1201 ggtcaggccc tcggccgcgg cacgcgcttg ggattcattc aactgctcgg tagacaacgt
     1261 gatgccggtc acgcgcgccc cataatcgcg cgcaagggtc aatgccaacc cgccccagcc
     1321 tgagccgatg tccagcaccg tgaggtctgg ccgatcgagg cacagcttgg cggcaatgtg
     1381 gcgcttcttc gcgatctgcg cctgttccag ggtttcgtcg ccacgcggga agtaggcaca
     1441 ggagtattgc cggtcgcgat cgaggaacag gctgtacagc cggccgttca gatcgtaatg
     1501 atgggccacg ttgcgttgcg cgcgcgtcgc ggggttatac tggtcaaggc ggcgcttgag
     1561 caggccgagc gcggaccgta tgcgtgcgat cgggtggccc tggctgctgg actccaggtt
     1621 cagcatcagc agatcgagca cgttgtagat accgcgctca tcaggaatca ggccgccgtc
     1681 catgtaacac tcgccgacgg cgagtgttgg attgagcacc atgcggcgga tcgcggcgtt
     1741 gtcgcgcagc gcgaaagagg cttcggggcc agagccctcc ggcccggtat aggtcgtggt
     1801 ccgcccgtcg ggccagcgaa ccatcagact gccgaacttg atgaagcgcc gcgtgatggc
     1861 gtcaagaact gcctgcatgt cggtaacaat ggcgcaattc tggcgtaatg gtcaaagggt
     1921 gtttgggccg cttggaatac ccctaggcgg ccgcctcagt tcacgctgcg gaagatcaca
     1981 gacgcgttgg tgccgccgaa accaaagctg ttcgacaggg cgtggtcgat ccgccggtcc
     2041 tgcgccgccc tagcgacacg gtcgattacg ctttcgcggc tgggctcctc aaggttcagg
     2101 gtgggtggcg ccacattgtc gcgcacagcg aggatcgaga agatcgcctc caccgcaccg
     2161 gcggcaccga gcaggtgccc ggtcgccgac ttggtcgacg acattgcaag tcccttggcc
     2221 gcggcaccaa acacgcgctc gacggcttcg agttccagat cgtcgccaag cggcgtggag
     2281 gtcccgtgcg cgttcacgta ctgcacctga tccgggctga cgcc
//
LOCUS       DATGEM010000238        17971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1034963, whole genome shotgun sequence.
ACCESSION   DATGEM010000238 DATGEM010000000
VERSION     DATGEM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 17971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17971
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034963"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VKQ27_07500"
     CDS             complement(<1..771)
                     /locus_tag="VKQ27_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88813.1"
                     /translation="MSATQASKDPVLVVLQLTGGNDYLNTVIPYNNGLYKDNRKAVGI
                     ADSDMVHLDQAHAIPQYMAPLKKFWDDDKLAIMHGVGYLDSPRSHFRSMDIWHTCEAD
                     KVGTEGWLGRVIREFDPKKENVVTAVSFGACLFRALAVPGVPVACVAGPLEQYGFLPT
                     IRDQAQRLKVLETFSHMYQPIEGSGVMDYLGTTGLDGLKGADILKVAPGKYKSNVKYP
                     NTTIAQKLRGIAQVHLAGLGTRVFYCDHGSFDSHAGQAP"
     gene            complement(806..2200)
                     /locus_tag="VKQ27_07505"
     CDS             complement(806..2200)
                     /locus_tag="VKQ27_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HLY88814.1"
                     /translation="MANQDDIALVAHLMRRAGFGAGREELEARAAKGYDATVEELLNP
                     DSQPPVDPYVLLRHQPASLLPGGQPPMGNVNYMYYLVNTKRPLEEKMALFWHHVFATG
                     NSKVDNYDQLLEQIEMFRQHGMGNYRDLLLKVAKNPTMLFWLDNNQNHGTAVNENWGR
                     ELLELFSLGASNYTEVDVREASRAFTGWTFETKLPRLPYGRFPWKFEYRPEDHDDGEK
                     TFLGHKGNFNGEDIINIIVQQPACAKFICRHLYNFFVADEPQVPAWQIEAPRDQAAID
                     SMCKVFMDSKCEMKPLLRHVFKSDFFKNARYKHLKSPAEVVVGTLRLVGGYELPKPGY
                     GELSMQPSYMGQDLLNPPSVEGWHTGQEWINSGSLMSRINFVAAQLGNPDLPGVKKII
                     ERLKAQGTKTPEQLVANSLDLLGPIEVSADTKKELTDAAKQQGDIRWEDAGAPQRAAE
                     MLQLIAATREYQFG"
     gene            2439..3503
                     /locus_tag="VKQ27_07510"
     CDS             2439..3503
                     /locus_tag="VKQ27_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY88815.1"
                     /translation="MFVQSKYAISFIDVANRRSVVVTLAELRYLVAIADLSHFGRAAE
                     RCRVTQPTLSGQLKKLEETLGVALVERTTHLVALTPIGASVVAHARRILAEADQISEL
                     VRHRDGALTSVLRLGIIPTLSPYILPLIFQRLHQNFPKLRLVLREDLTANLLSALDTY
                     TLDVLLIALPDRVDGYRAMPLFVEPFCFACPPGHKLSNRSVVTEQELAHECLLLLDEG
                     HCLRDQALEVCGNHFKEGPRSSDDFRATSLETISEMVVAGLGCTLLPAMAVPYLTARH
                     RRLEVRPLVASKAYRRIGLLWRSSFPQADDLEELGQFIRDLLPDSVEPIGHGGARTRG
                     THARSPQSRAHALSAKSHRL"
     gene            3648..3893
                     /locus_tag="VKQ27_07515"
     CDS             3648..3893
                     /locus_tag="VKQ27_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88816.1"
                     /translation="MERMGNRDLQMCNRSAGPVVRAGILEGIQQSQVRFSITVPSCRH
                     LAEQDGRLSLIFLFGHACEYQIAATGNHLVGDSTEQI"
     gene            4177..5835
                     /locus_tag="VKQ27_07520"
     CDS             4177..5835
                     /locus_tag="VKQ27_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY88817.1"
                     /translation="MAEIKGADLLAKSLVEQGVQYMFGVVGFPVGPLAEAAQRAGLPY
                     IGMRNEQTASYAAGAVGYLTGRPGSCITVTGPGVIHGLAGLANAKENCWPMLLIGGAS
                     ETYRNGMGAFQEERQVLIATPVSKWAHAIEHVNRIPFYVEMAVRQSIYGRPGPSYLDI
                     ADDLIGGSCDVDKVVEAEKCPDPPRIQTLPQYVDQALDVLQSAQRPLVIIGKGMAYSH
                     AEDEVRAFIERTQLPFLASPMGKGVMPDDHPLSVGAARSLALQQADVVFLMGARFNWI
                     MHFGLPPRYSKDVRVIQLDISPEAMHQNKPAEVAMVGDGKAIIGQINEALKSRQWFYP
                     KETPWRQAIAKKSAENAAMIQPQKDDNSAPGGYYRLLKDVSAWVPKNAIICSEGASTM
                     DIGRTQLNNYAPRQRLDAGSYGTMGVGLGFVIAACVVHPDRPVVHVSGDSAIGFSGME
                     METLCRYGMPAKIVVFNNGGIGPGGGDIPDNAMLRMKPNSLIYGARYDLMMEAFGGKG
                     FYVEDPKDLRGALDEAMNTRGPALVNVKLSLGSQRKAQEFRWHS"
     gene            5910..7172
                     /locus_tag="VKQ27_07525"
     CDS             5910..7172
                     /locus_tag="VKQ27_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88818.1"
                     /translation="MVYLYPSVCICGSNFLTTPVSTYRALLAAPDVRALLLAALLARL
                     AGRMFALAIVLYALARFSSPELAGWLAFAAVAPGLLISPIAGALIDRVGSAWAITVDM
                     AASAILLATLALADRIGAAEPALVLLLTGAYSLTSPLSFAGIRALLPRLVPTDALGRA
                     NALDTALHGLTDVIGPASAGVLVGFAGSIAALCLIAAIYAAAAISLARVPHLAGRMPG
                     LRALLALAWFGLRRVVAQPTLRGLAVSYSLYEVCWGILVVVVPVFTAQRFGSGAGATI
                     AGLLWAGLGLVGAIAALVAGHLPAADRERRMMAIGMLLTAASAWPISAMFGLPGLAFG
                     LMLIGAAAGPIDVGVLTLRQRRTDPAELGRVLSVSMSLNLAGGPLGSALGGLLVVWSL
                     SGTFTIAALAATLGACAVSLIPGGTDRA"
     gene            complement(7306..7827)
                     /gene="pcaG"
                     /locus_tag="VKQ27_07530"
     CDS             complement(7306..7827)
                     /gene="pcaG"
                     /locus_tag="VKQ27_07530"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HLY88819.1"
                     /translation="MPIATASQTIGPYWHLLEDPSWADLTRFGAEGEKITLTGAVLDG
                     DGKPCADACVEIWQSDPAASDTFPGFGRSNTDRDGVYRFTTVKPGPVPGRGNTQQAPH
                     IALCILSRGLMRALMTRAYFQGDALNEADPLLMSIDEPARRGTLIAKPAGAGTWRMDV
                     RLQGDGETVFLDL"
     gene            complement(7831..8532)
                     /gene="pcaH"
                     /locus_tag="VKQ27_07535"
     CDS             complement(7831..8532)
                     /gene="pcaH"
                     /locus_tag="VKQ27_07535"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HLY88820.1"
                     /translation="MADPIPFRPHAPGTQPPYDAPAYLGTTKRHPKQKLHPLPQTITE
                     TTGPRFSPSRFPPMADLTMAAGKPAIGERIIVAGTITDEAGRPVPNTMVEIWQCNATG
                     RYDHPADQHDAPIDPNFHGAGRVFTDGAGRYRFLTIRPGAYPWPNHKNAWRPNHIHFS
                     YFGPAFATRLVTQMYFPGDPLLAFDPIFHAVPDEHARNRLVAVFDLDITEQDYALGYR
                     FDVVLRGRDATPMEH"
     gene            8683..9324
                     /locus_tag="VKQ27_07540"
     CDS             8683..9324
                     /locus_tag="VKQ27_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88821.1"
                     /translation="MGATKIMVIRDAEKPAPYDGANYSGVDAVGTTCGTDGAASLVTI
                     GWERAGALVTLFAPPRGPKTPILAQPQHIYASDPTAKESGKLPSQGPYETVTAIAAKL
                     GLTVNTKHKKSDYPRMVQDALGQDGVVPICWQHEDIPLDADGNVPGISQCILTDTGTQ
                     AKLGVPKSWPKGPNGVARYDLARVFDRPSGIGPITGFNFFAQMLLPGDTTAPT"
     gene            complement(9317..9925)
                     /locus_tag="VKQ27_07545"
     CDS             complement(9317..9925)
                     /locus_tag="VKQ27_07545"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HLY88822.1"
                     /translation="MTDWLTRCPLVAILRGVKPEEAVAIGLALERQGIAIVEVPLNSP
                     RPMESIALLAREFGERLLIGAGTVMTPAQVAEIAAAGGKLIVTPHADAAIVRVAKQHG
                     LIALPGFFTPAEAFAMLAAGADALKLFPSEAASPAVLRALRAVLPSGMAVLPVGGIDA
                     ANIPAWRAAGAAGFGIGSAIYKPGDSPETVGAKAHALVAALM"
     gene            complement(9922..10806)
                     /locus_tag="VKQ27_07550"
     CDS             complement(9922..10806)
                     /locus_tag="VKQ27_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HLY88823.1"
                     /translation="MIGVDWGTTSFRAFRMAADGTIRDRRIALKGILNVPDSRFADTL
                     REEIGPWLAAGENHVLLSGMIGSRQGWKEAPYVACPAGAPEIAASLVEIEFDWGQVKL
                     VPGLSGTDEAGVAEVMRGEETQVFGVLTAMGGSGLACLPGTHSKWVRVENSRIISFTT
                     HMTGEAFSALRGHTILGRMMRDGPSNGAPFDAGVRRAGDPGGLLHHIFGARAEVLAGR
                     LAETDAPAYLSGILIGHEVRAALAGRQGEVVHVIGSPELTALYGRAISAHGGFAERHD
                     GEAAARGLARIGEHATWR"
     gene            complement(10819..13725)
                     /gene="putA"
                     /locus_tag="VKQ27_07555"
     CDS             complement(10819..13725)
                     /gene="putA"
                     /locus_tag="VKQ27_07555"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HLY88824.1"
                     /translation="MEPLLTIAASSPGVAVRARTLVNAARASHRPGADVTDFLAEYGL
                     GTAEGVALLCLAEALLRIPDAATADALIEDRIAAADWQKHLGQGDSLAVNASAWAFML
                     TGRVLNLDNVAGVIGAMIRRLGEPVIRTALRRGMRMLARQFVMGRTIEEAVGRANRKL
                     RYSYDMLGEAAKTHADAERYQQAYLHAISVIKGANAGISVKLSALHPRYEPLQAVQCV
                     PALIGSLAGLALAAKEAGIGLTVDAEEADRLEISLDIFAAVLGDPRLAGWDGLGLAVQ
                     AYQKRAFPLIEWVAALGRRTGHRIPTRLVKGAYWDTEIKLAQVQGYSDYPVFTRKAAT
                     DVSWLACARRMMQHSDAIYPAFATHNAHSLAFVLETATADFETQRLHGMGEALYHGAA
                     WPVRVYAPVGTHEDLLAYLVRRLLENGANTSFVHRLVDPSVPEEAIIADPVIKAGPPN
                     SRIPLPRVLYPDRLNSAGFDLADRDAAPALLAALHRPPTVAGTREIRNPADRRGLVGA
                     INDASTEVIDAQLGALSRGWRDWDRAGGAHRAQVLERAADMIEAARDELLSLLVREGG
                     KTLGDGIAEIREAADYCRWYALLARRDFAAPRELAGPTGERNTWELGGRGIFAAISPW
                     NFPLAIFTGQVAAALAAGNAVAAKPAEQTPLVAARAVALLLEAGVPRNALALLPGDGA
                     VGQALVRDPRVAGVAFTGGTETAQSIARALATRPGPIVPFIAETGGLNAMIVDSSALA
                     EQVVTDVLASAFGSAGQRCSALRLLCLQEDIADRVLHMLQGATETLVIGDPIDVATDV
                     GPVIDEDARAALEQACATLGAPLFTLPLPPGTGHGSFFAPRAYLLRHASELRMEIFGP
                     VLCVVRYTADGLNELMDAIDRNGYGLTLGIHSRIEHTQRRIAARLRIGNTYVNRNQIG
                     AVVGVQPFGGRGLSGTGPKAGGPVTLYRFAEERVVSINTAAAGGNAALLASDEG"
     gene            complement(13831..15276)
                     /gene="hydA"
                     /locus_tag="VKQ27_07560"
     CDS             complement(13831..15276)
                     /gene="hydA"
                     /locus_tag="VKQ27_07560"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HLY88825.1"
                     /translation="MADYDLVIRGGTVVTAADTYRADVAIRGGRIAAVGDKLDGTDTL
                     DAGGLYVMPGGVDTHCHIEQLRPNGGSDEETFVTGSAACLAGGTTTVITFAAQFKGHG
                     ILDTLAEYHRRAKKAMVDYSFHQIITDPSDVVVRDEIPQVVASGVRSLKVFLTYEPSH
                     LDDREYIRVLAAARRTGALVTVHCENYEAIRWRTSALMADGRSAPKYHSWSRPKVVER
                     EATYRAVALAEMVDQPIQVFHVSCSEVAEEIARAQARGVKVWAETCPQYFVLGAADMD
                     RPGFEGAKFMCSPSPRDAATSAALWNDVKRGTLDVVSSDHSGASYEGEGGKRIHGNNA
                     PFPDIPNGVPGLAARLPLVFSEGVAKGRIDLNTYVRLISTNPAKLFGLYPRKGTIAPG
                     ADADLVLWDAGKRVTITNELMQHTIDYTPYEGMEVTGWPVATVKGGRVAMRDGKVQAE
                     PGTGQFLARAPYDLIKPTGNLPDGFDAAQFI"
     gene            complement(15292..16827)
                     /locus_tag="VKQ27_07565"
     CDS             complement(15292..16827)
                     /locus_tag="VKQ27_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88826.1"
                     /translation="MAASLIRSRAMITRALDRYRWEEIADGAVLQEDGMITAVGTFAD
                     LSRLHPTVPVIGSGDDILLPGFINGHHHVGLTPVQLGSPDMPLELWFVTRMVMRNVNP
                     YLDTLYSAFEMIASGITTVQHLHGWAPGGLPEVEARCEEILRAYQDVGMRVSYSFSVR
                     DQNRLVYQDDAAFVTSLPMELRGPVQRWFDRFQLSLDDFFAVFEDLHGRHHNDRRVKV
                     QLSPANLHWCSDAALTRFAETSRKYEVPMHMHLVETAYQKQYARRRGGGTAVEYIDRF
                     GLLGPHMTLGHGVWLNEKDIDRLAETGTCVCHNCSSNFRLRSGVAALNAFEAKGINTA
                     IGIDEAGINDDRDMLQEMRMVLRAHRVPGMEDDVVPTAAQVFRMATVGGAKTTAFGER
                     IGALEPGRAADVVLIDWRSLSWPYLDEETPTLDAVLQRAKSARTVICDGEVIYDEGRF
                     TKVDRDAALRELHQDLQRALADDEMERRKLSKALLPHVKAFYANYFDPAEHAPFYRPS
                     SRI"
     gene            16870..>17971
                     /locus_tag="VKQ27_07570"
     CDS             16870..>17971
                     /locus_tag="VKQ27_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLY88827.1"
                     /translation="MRLSHKTIRPTGRPITIQFDGRPIPALEGETIAAALSAAGIVAF
                     RRTLTGAPRGLHCGMGACFDCVVSVDGRIGQRACMTKAADGMIVSGEPTTPLAPLAPE
                     PEAGESPEQGCDVLVIGGGPAGLSAAIAAAESGASVVVLDERTATGGQYAKPLADSHV
                     DAAPDAQFRLGIDLRDQALAAGARIETDATVWGGFAPDEIAALVQGRAITYRPRRLII
                     APGAHERPVPLPGWTLPGVMTTGSLQTLVRAQRVCPGEHVLIAGSGPLNLQLACELLA
                     CGVKPLAVAESAPRPSASAWRQAWRMARTAPDLLREGIGMLVTLKRAGVPVLWSARIK
                     QLTGDGRVQTAHVAGRTFDRSYNVDVVALNLGF"
BASE COUNT         3256 a         6090 c         5365 g         3260 t
ORIGIN      
        1 cggggcctgg ccggcatgtg agtcgaagct gccgtggtcg cagtagaaga cccgggtgcc
       61 gaggccggcg agatgcacct gggcgatgcc gcggagcttc tgcgcaatcg tggtgttcgg
      121 atacttcaca ttcgacttgt acttgcccgg cgcgaccttc aggatgtcag cgcccttcag
      181 accatcgagg cctgtggttc cgaggtagtc catgacaccg ctgccttcga tcggctgata
      241 catgtgggag aaggtctcca ataccttcag ccgctgcgcc tgatcgcgga ttgtgggcag
      301 gaagccgtac tgctccaggg gcccggccac gcaggcgacg ggaacgcccg gtaccgccag
      361 cgcgcggaac aggcatgcgc cgaagctcac cgcggtgacg acgttctcct tcttcgggtc
      421 gaactcacgt atcacgcggc caagccagcc ttctgtgccg accttatcgg cctcacaggt
      481 atgccagatg tccatggagc ggaagtgcga gcgcggggaa tcgagatagc cgacgccatg
      541 catgatggcg agcttgtcgt catcccagaa cttcttcagc ggcgccatgt actgcgggat
      601 ggcgtgcgcc tgatccaggt ggaccatgtc gctgtcggcg atgccgaccg ccttgcgatt
      661 gtccttgtac aggccgttgt tgtacgggat caccgtgttc aggtagtcgt tgccgcccgt
      721 gagctgcaac acgaccagta cagggtcctt cgatgcttgc gttgccgaca tagtagcttc
      781 tctcctatcc gttccgcatc atgtttcagc cgaactgata ctcgcgcgtt gccgcgatga
      841 gctgcagcat ttcggccgcg cgttgtggtg caccggcgtc ttcccagcgg atgtcgcctt
      901 gctgctttgc cgcgtcggtg agctccttct tggtatcggc gctcacttca atcgggccga
      961 gcagatccag ggagttggcg acgagctgtt cgggcgtctt ggtgccctgg gccttcagtc
     1021 gctcgatgat cttcttcacg cctggcagat cgggattgcc gagctgtgcg gcgacaaagt
     1081 tgatgcgcga catcagcgag ccgctgttga tccactcctg accggtatgc cagccctcga
     1141 ccgacggcgg gttgagcaga tcctgcccca tgtatgaagg ctgcatcgac agctcgccat
     1201 aaccgggctt cggcagttca tagccaccga ccagacgcag tgtgccgacg accacttcgg
     1261 ccggcgactt caggtgcttg tagcgggcgt tcttgaagaa gtccgacttg aacacgtgac
     1321 gcagcagcgg cttcatctcg cacttcgaat ccatgaacac cttgcacatc gaatcgatgg
     1381 ccgcctgatc acgtggcgcc tcgatctgcc atgcaggcac ttgcggttcg tcggcgacga
     1441 agaagttgta caggtgccgg cagatgaact tcgcgcaagc cggctgctgc acgataatgt
     1501 tgatgatgtc ctcgccgttg aagttgccct tgtggccgag gaacgtcttc tcgccatcgt
     1561 cgtggtcttc cggccgatac tcgaacttcc acgggaagcg gccgtacggc aggcgcggca
     1621 gcttggtttc gaaggtccaa ccggtgaagg cacgcgacgc ttcgcgcacg tcgacctcgg
     1681 tgtagttgct cgcaccgagg ctgaagagtt cgagcagttc gcggccccag ttctcgttca
     1741 ccgccgtacc atgattctgg ttgttgtcca gccagaaaag catcgtcggg ttcttcgcca
     1801 ccttgagcag caggtcgcga tagttgccca tgccgtgctg gcggaacatc tcgatctgtt
     1861 ccagcagctg gtcgtagtta tcgaccttgg aattgccggt agcgaacacg tggtgccaga
     1921 acagcgccat cttctcttcc agcgggcgct tcgtgttcac cagatagtac atgtagttga
     1981 cgttgcccat tggcggctgg ccgccgggca gcaacgacgc cggttgatgg cgcagcagca
     2041 cgtagggatc tactggcggc tgactgtctg ggttcagcaa ttcctcgact gtcgcgtcgt
     2101 agcccttggc ggcacgtgct tccaactctt cgcggccagc accgaaaccg gcgcgtcgca
     2161 tcaaatgcgc aaccagcgcg atgtcgtcct gatttgccat atccgttcct cccgaccaaa
     2221 tgagtgataa tcgtatcctg ggctgctacg accccttctc tgttcgctgt cacatcatcg
     2281 ctcgcaagct cgccagtcag gggaccgaca tcaggtgcgt tgcgtcacct cgtcgccatg
     2341 cggcccttcg ctgatccgac ctgccaaacc acagcaagcc cgcagccaga aatgacagcc
     2401 agccacttgt attgtggggg agccttgccg ggaggacgat gtttgtccaa tctaaatatg
     2461 cgatttcgtt tatagacgtc gccaatagga gaagcgtcgt cgtgaccttg gccgaactac
     2521 ggtatttggt cgctatcgct gacctttcgc actttggccg agcagccgag cgctgccgcg
     2581 tgacacaacc cacgctcagt ggccaactga agaagttgga ggaaaccctt ggtgtcgcgc
     2641 tggtcgagcg cacgacacac ctcgtggccc ttaccccgat cggcgcaagc gttgtggccc
     2701 atgcgcgccg cattctcgct gaagccgatc agatatccga actggtgcgt catcgcgatg
     2761 gggcactcac cagcgtgctg cgtctcggca tcatcccgac gctaagccca tacattctgc
     2821 ctctgatctt ccagcgcctg caccagaact ttcccaagtt gcggctcgtg ttgcgggagg
     2881 atctgacggc caacctgttg tccgcgctcg atacctacac actggatgtc ctgttgatcg
     2941 cactccccga tcgggtcgac gggtatcgcg cgatgccgct gttcgtggag ccgttctgtt
     3001 tcgcttgccc acctgggcac aagctgagca accgttccgt ggtgacggaa caggaattgg
     3061 cacatgagtg cctgttactg ctggacgaag ggcattgcct gcgcgatcag gcgttggagg
     3121 tatgcggaaa ccacttcaag gaggggcctc gatcgagcga tgatttccgg gcgacaagcc
     3181 ttgagacgat ctcggaaatg gtggtggcag ggcttgggtg cacgttgttg ccggccatgg
     3241 cggtgccgta tctgaccgcg cgtcatcggc ggctcgaggt acgcccgctg gttgcttcga
     3301 aggcttacag gcgaattggc ctgctgtggc gatcgagttt cccgcaggcc gatgatctcg
     3361 aggaattggg ccaatttatt cgtgacctgc tgccggacag tgtcgaacct atcggccatg
     3421 gaggcgcacg tacccgaggc acgcacgcca gatcgccgca gtcgcgggcc catgccttaa
     3481 gtgccaaatc ccacagactg tgactgtgtg gcctgctcga gtgcttgctc gaaccggcac
     3541 gctcgcgcgc ggctggcttc ggcgaccatt tcaaaccagt cggcgatctc atcgaaccct
     3601 tcgtctcgag ccgttcgggc catccctgga tacatgacag cgtattcgtg gagcgcatgg
     3661 gcaatcgaga ccttcagatg tgcaatcgca gcgccggtcc cgtggtgcgc gccggcatcc
     3721 tcgagggcat ccagcaatcg caggtgcgct tcagcattac tgttccgtcg tgccgacatc
     3781 ttgcggaaca ggacggccgc ctgtcgctga tcttcctctt cggccacgcg tgcgaatacc
     3841 agatagcggc gactggcaat cacctcgtgg gcgacagcac cgagcagatt tgagtacgtg
     3901 cggctggcag tgatttcggc cattggtttc ctccctcccg ctcgacgagg cgggattgcg
     3961 ggtgggacca gcggctctat cggggccgtt cggcaaggtg ccttggtccc acgtgcgctt
     4021 gagaggcaaa cagagcacgc tcacgccttg gcggtcaatt gcatctaatc gcgcttcccc
     4081 aagcctatgc gagcgccggt ttcgcacgcc tgggatttga cgcgccaccg cacatcgttc
     4141 agcgtactgt catccaagtg gttgggaggg atatccatgg ctgaaatcaa aggcgcggac
     4201 ctgctcgcga agagtctggt ggagcaaggc gtgcagtata tgttcggtgt ggtcgggttc
     4261 ccggtgggtc cgctcgccga ggccgcacaa agggccggcc tgccgtatat cggcatgcgc
     4321 aacgaacaga ctgcgtccta cgctgccggt gccgttggtt atctcacagg ccgtcccggc
     4381 tcctgcatca cggtgaccgg ccccggcgtg attcacggtc tcgctggcct cgccaatgcg
     4441 aaagagaact gctggccgat gctgctgatc ggcggcgcgt cggagaccta tcgtaacggc
     4501 atgggcgcgt tccaggagga acgccaagtc ctgatcgcca ctcccgtcag caagtgggcg
     4561 catgcgatcg agcacgtgaa ccgcattccg ttctacgttg aaatggctgt gcgccagtcg
     4621 atttatggcc gtcccggtcc gtcgtatctc gacatcgcgg acgacctgat cggtggctcg
     4681 tgtgatgtgg acaaggtggt ggaggcggaa aaatgccccg acccgccgcg tatccagacg
     4741 ctgccgcaat atgttgatca ggcgcttgac gtgcttcaat cggcgcagcg gccgttggtc
     4801 atcatcggca aggggatggc gtattcgcac gccgaggatg aagtgcgcgc attcatcgag
     4861 cgcacacaac taccgttcct cgcctctccg atgggcaagg gggtgatgcc ggacgaccac
     4921 ccgctgtcgg tcggcgccgc gcgcagcctg gcgctgcagc aggcggacgt ggtgttcctg
     4981 atgggcgcac gcttcaactg gatcatgcat ttcggcctgc ctccgcgtta ctcgaaggac
     5041 gtgcgggtga tccagctcga catctcgccg gaggcgatgc accagaacaa gccggctgaa
     5101 gtggccatgg tgggtgacgg caaggcgatc atcggacaga tcaatgaggc gctgaagagc
     5161 cgccagtggt tctatcctaa ggagactccg tggcgccagg cgatcgccaa gaagtcggcc
     5221 gagaacgccg cgatgatcca gccgcagaag gacgataact cggcgccggg cggctattat
     5281 cggttgctga aggacgtctc cgcctgggtt ccgaagaacg ccatcatttg cagtgagggc
     5341 gcgagcacga tggatatcgg ccgcacccag ctgaacaact acgcgccgcg ccagcgtctt
     5401 gacgcgggta gttacggcac aatgggtgtt ggcctcgggt tcgtcatcgc cgcctgcgtt
     5461 gtgcaccccg atcggcctgt tgtgcatgta tcgggtgact cggccatcgg cttctccggc
     5521 atggagatgg agactctctg ccgctacggc atgccggcga agatcgtggt gttcaacaac
     5581 ggcggcatag gaccgggcgg cggcgatatc ccggacaatg cgatgctgag gatgaagccg
     5641 aattcgctga tctatggtgc ccgctacgac ctgatgatgg aagcgttcgg cggcaagggc
     5701 ttctatgtcg aggatccgaa ggacctgcgc ggcgctctgg atgaggcgat gaacacccgt
     5761 gggccggcgc tggtcaacgt gaagctgagc ctcggctcac agcggaaggc ccaggagttc
     5821 cgctggcaca gctgaaatga atggaaccac agatggacgc agataaacac agataaactg
     5881 atgcgtgcgg gcgagacgct gcccgcacct tggtctatct gtatccatct gtgtgtatct
     5941 gtggttcaaa tttcctgacc acgccggtct ccacctaccg agccctgttg gccgcgcccg
     6001 acgtgcgcgc cctgttactt gccgcactgc tggcacggct cgccggccgc atgttcgcac
     6061 tggcgatcgt gctttacgcg ctcgcccggt tcagttcgcc cgagctcgca ggatggctcg
     6121 cgttcgccgc ggtagccccc ggactgctga tcagcccgat tgctggagcg ttgatcgatc
     6181 gcgtcggctc cgcatgggcg attaccgtgg acatggcagc gagcgcgatc cttctggcaa
     6241 cactggcgct cgctgatcgg atcggcgcag ccgagccagc actcgtgctg ctgctgaccg
     6301 gcgcatattc attgaccagt ccactcagct tcgccggcat tcgcgctctg ttgccgaggc
     6361 tcgtgccaac agatgcactc ggccgtgcca acgcgctgga tacagcgctg cacggactga
     6421 ccgacgtcat tggcccggca tcggctggcg tactcgtcgg gttcgccgga tcaatcgcgg
     6481 cactctgtct cattgcggcg atctacgctg cagccgcaat cagcctggca cgcgtgccac
     6541 atttggcagg tcgcatgcca ggtctgcgcg ctctgctcgc gctggcatgg ttcggactgc
     6601 gacgcgtcgt cgctcagcca acgctgcgag gactggcggt ctcctactca ctctacgaag
     6661 tgtgctgggg catcctcgtc gtcgttgtgc cagtgttcac cgcacagcgg ttcggcagtg
     6721 gcgcaggtgc gacaatcgcc ggattgctat gggcgggatt ggggctggtc ggcgcaattg
     6781 ccgcgctggt cgcgggccat cttcccgccg cagaccgaga gcgccgaatg atggcaatcg
     6841 gcatgctgct gacagccgcg tccgcatggc caatctcagc tatgttcgga ctgccgggac
     6901 tggccttcgg cctgatgctg atcggcgccg ccgccggccc catcgatgtc ggtgtgctga
     6961 cgttacgaca gcgccggacc gatccggcag aactgggccg cgtgctgtcc gtttcaatga
     7021 gcctgaacct tgccggcggc ccactgggat cagcactcgg tggattgttg gtggtctggt
     7081 ctctgtcggg aacgttcacc atcgcggcgc tggcagcgac actgggagcc tgcgccgtct
     7141 ctctgatccc cggagggacc gaccgggcat agtccaaggc ggctacgtca tggcccattt
     7201 ttggcgctcc cgtcattgcg accaccgcga aagcgcaggg aagcaatcct gaacgagatt
     7261 gcctcgcttc gatcgcaatg acggaacccg gcaatccgac atcacttaca ggtctaggaa
     7321 caccgtctcc ccgtcgccct gcagccgcac atccatccgc cacgtgccgg cacccgcggg
     7381 cttggcgatc agcgtgccgc gccgagccgg ttcgtcgatc gacatcagca gcgggtccgc
     7441 ctcgttcagc gcgtcacctt ggaaatacgc ccgcgtcatc agcgcacgca tcaatccgcg
     7501 cgacagtata cacagagcga tatgcggcgc ctgctgcgta ttcccccgtc ccggcaccgg
     7561 ccccggcttc accgtggtga accgatagac cccatcgcga tccgtattgc tgcgcccgaa
     7621 tccaggaaac gtatcggacg ccgcaggatc agactgccag atctccacgc acgcatcggc
     7681 gcacggcttg ccatcgccat ccagcaccgc gccggtcagc gtgatcttct cgccctcggc
     7741 gccaaaccgg gtcaggtccg cccaggaagg atcctccagc agatgccagt acggcccgat
     7801 cgtctggctc gcggtcgcga tcggcatcgc tcagtgctcc atcggcgtgg catcacggcc
     7861 gcgcagcaca acatcgaacc ggtacccgag tgcgtaatcc tgctcagtga tgtccagatc
     7921 gaataccgcg accagccggt tccgcgcgtg ctcatccggc accgcatgga aaatcggatc
     7981 gaacgcaagc agcggatcgc ctggaaaata catctgcgtc accagacgcg tcgcgaacgc
     8041 cggcccgaaa tacgagaaat ggatgtggtt cggccgccac gcattcttgt ggttcggcca
     8101 tggatatgct cccggccgga tggtcagaaa acggtaccgc ccggccccat cggtgaacac
     8161 ccgtccggcc ccatggaaat tcggatcaat cggcgcgtcg tgctgatcgg ccggatggtc
     8221 gtaccgcccg gtcgcattgc actgccagat ctccaccatc gtgttcggca ctggccgacc
     8281 ggcttcatcg gtgatcgtgc ccgcgacaat gatccgctcg ccgatcgctg gcttcccggc
     8341 cgccatcgtc agatcagcca tcggtggaaa gcgcgagggg gagaaccgcg gtcccgtcgt
     8401 ctccgtgatg gtctgcggca gcggatgcag cttctgcttc ggatgccgct tggtcgtgcc
     8461 gagataggcc ggcgcgtcat acggcggctg cgtgccaggc gcatgcgggc ggaatgggat
     8521 gggatctgcc atcaggctcc tccacggtaa ggcgccgcag gctgtcacac cggacggccc
     8581 gctgcaattg atctgcggca acgcatcgcc agccccaaaa tatgttgttc ccggaacagc
     8641 aaggcgcgca tcacggctgt ccaggcctga gagaaacggc caatgggtgc cacaaaaatc
     8701 atggtgatcc gggacgcgga aaagccggcc ccctacgacg gggccaatta ttcaggcgtc
     8761 gatgcggtag gaacgacttg cggcaccgac ggtgcggcgt cgctcgtcac catcggctgg
     8821 gaacgtgcgg gcgcactcgt cacgctgttc gcgccgcccc gggggccgaa gacgccaata
     8881 ctggcgcaac cgcagcacat ctatgcctct gaccccaccg cgaaggagag cggtaagctg
     8941 cccagccagg gcccctatga gacagtgacc gcgatcgctg ccaagctcgg gttgacggtc
     9001 aatacgaagc acaagaagag cgactatccg aggatggtgc aggatgccct cggtcaggat
     9061 ggcgtcgtgc cgatctgctg gcagcatgag gacatcccgc tggacgcgga cggcaacgtc
     9121 cctggcatca gccaatgcat cctcaccgat acgggcacgc aagctaagct gggtgtcccg
     9181 aaatcctggc caaagggccc gaacggtgtc gcacgctatg atctagcccg agtgttcgat
     9241 cgaccgtcgg gcatcggtcc gatcaccggt ttcaatttct tcgcgcagat gctgctgcca
     9301 ggcgatacaa ctgcgcctac atgagcgccg ccaccagcgc atgtgccttg gcgccgacgg
     9361 tttccggact gtcgccgggc ttgtagatcg ccgagccgat gccgaagcca gcggcccccg
     9421 ccgcgcgcca ggccgggatg ttggccgcgt cgatgccacc caccggcagc accgccatcc
     9481 cactcggcaa tacggcacgc aacgcgcgca acaccgcggg gctggccgcc tcggaaggga
     9541 acagcttcag cgcatctgcg ccggcggcca gcatcgcgaa cgcctcggct ggcgtgaaga
     9601 acccgggcag cgcgatcaac ccatgctgct tcgccacgcg aacaatggca gcgtcggcgt
     9661 gaggcgtgac aatcaacttg ccgccagccg ctgcaatctc cgccacctgc gccggggtca
     9721 tcaccgtgcc ggcgccgatc agcagccgct cgccgaattc acgcgcgagc agagcgatgc
     9781 tctccatcgg acgcggcgag ttcagcggca cctcaacgat ggcgataccc tgccgctcca
     9841 gcgccaaacc gatcgccacc gcctcttcgg gcttcacgcc gcgcaggatg gcgaccaacg
     9901 ggcagcgcgt cagccagtcg gtcatcgcca cgtggcatgc tccccgattc gggccaggcc
     9961 gcgcgccgcc gcctcgccat cgtggcgttc ggcaaacccg ccatgcgccg aaatcgcgcg
    10021 tccatacagc gcggtgagtt cgggcgagcc gatgacgtgc acaacctcac cctggcgtcc
    10081 cgccagcgcc gcgcgaactt catggccgat caggataccc gacagatagg cgggcgcgtc
    10141 ggtctccgcg agccgccccg ccagcacctc cgcacgcgcg ccgaagatgt gatgcagcaa
    10201 accgccggga tcgcccgcgc gtcgcacgcc ggcgtcgaac ggcgcgccat tcgatgggcc
    10261 atcgcgcatc atccgcccca ggatcgtgtg gccgcgcaac gcactgaacg cttctccggt
    10321 catgtgcgtg gtgaagctga tgatgcggct gttctccacc cgcacccact ttgaatgcgt
    10381 ccccggcagg cacgccagcc cgctgccgcc catcgcggtc agcacgccga agacctgcgt
    10441 ttcctcgccg cgcatcacct ccgcgacgcc ggcttcatcg gtgcccgata gaccgggcac
    10501 cagcttcacc tgcccccaat cgaactcgat ctccaccagc gaggcagcga tttccggcgc
    10561 gccggccgga caggcaacgt aaggcgcctc tttccagccc tgccggctgc cgatcatgcc
    10621 cgacagcagc acgtggttct cgccggccgc gagccatgga ccgatctcct cgcgcagcgt
    10681 gtccgcaaag cggctgtcgg gcacgttgag aatgcccttc aacgcgatac gtcggtcccg
    10741 gatcgtgccg tccgcagcca tccggaatgc gcggaaactc gtggtgcccc aatcaacgcc
    10801 gatcatggtt cgtttcccct agccttcatc cgacgcgagc agcgccgcat tgcctcccgc
    10861 cgctgcggtg ttgatggata cgactcgttc ctcggcaaat cgataaagcg tgactggccc
    10921 gccagctttt gggcccgttc cggacaggcc acgcccaccg aacggctgta caccaaccac
    10981 ggcgccgatc tggttgcggt tgacgtaggt gttaccgatg cgcagccgag cggcgatacg
    11041 gcgctgcgtg tgttcgattc ggctatggat gccgagcgtc aagccgtagc cattgcggtc
    11101 gatggcatcc atcagctcgt tcaatccatc ggcagtgtaa cgcacgacgc acaggaccgg
    11161 cccaaagatt tccatccgca gttcgctggc atgccgcaaa agataggcac gaggtgcaaa
    11221 gaagctgcca tgcccggtgc ctggaggcaa aggcagcgtg aacaatggcg cgcccagtgt
    11281 ggcgcaggct tgttccaacg cagcacgcgc gtcttcgtcg atcaccggcc ccacatccgt
    11341 ggcaacgtcg atcggatcgc cgatcaccag cgtctccgtc gcgccttgca gcatgtggag
    11401 cacgcgatcg gcaatgtcct cctgcaggca gagcagccgc agcgccgagc aacgctgccc
    11461 tgccgaacca aaagcactcg cgagcacatc ggtgaccact tgctcggcca acgcggagct
    11521 gtcgacgatc atggcattca gcccaccggt ctcggcaatg aacggcacaa tcgggcccgg
    11581 tcgtgtggcg agcgccctcg cgatggattg cgcggtctct gtcccgccgg tgaaggcgac
    11641 cccagcaaca cgcggatcgc gcaccaaagc ctggccgaca gccccatcgc ccggcagcag
    11701 cgctagcgcg ttccttggca cgccggcctc cagcagcagt gcaaccgcgc gtgctgcgac
    11761 cagcggcgtc tgctcggcgg gcttcgccgc aacggcgttt cccgccgcga gcgccgctgc
    11821 aacttgaccc gtgaagatcg ccagcggaaa gttccacggc gaaatggcag cgaatatgcc
    11881 gcgtccgcca agctcccagg tattgcgctc gccggtcgga ccggcgagtt cgcgtggcgc
    11941 agcgaagtcg cggcgcgcga gcagtgcata ccaccggcag taatccgcag cctcgcggat
    12001 ttcagcaata ccgtctccga gcgtcttgcc gccttccctc accagcaatg acagcagctc
    12061 gtcgcgcgca gcctcgatca tatcggcagc gcgttccagc acctgggcgc ggtgcgcacc
    12121 gcccgctctg tcccaatcgc gccacccacg cgacagcgca ccgagctgcg catcgatcac
    12181 ctcggtggac gcatcattga tcgcgccaac cagtccgcga cgatcggcag gattgcggat
    12241 ttcgcgcgtg ccggcgacag ttggcggtcg atgcaacgct gccagcagcg ccggcgcggc
    12301 gtcacggtca gccagatcaa acccagcgga attcagccga tcgggataaa gcacgcgcgg
    12361 cagcggtatg cgcgagttcg gcggcccggc cttgatgacc ggatccgcga tgatcgcctc
    12421 ctccggcacc gacggatcga ccagccgatg cacaaagctg gtgttcgccc cattttccag
    12481 cagccgccgc accagatacg ccagcagatc ctcgtgcgtc cctacgggcg catacacacg
    12541 caccggccac gcggcgccat gatacagcgc ctcccccatg ccatgcagcc gctgcgtctc
    12601 gaaatcggct gtggccgtct ccagcacaaa cgccaggctg tgcgcgttgt gcgtcgcaaa
    12661 cgcgggatag atcgcgtcgc tgtgctgcat catccgccgg gcgcaggcga gccagctcac
    12721 gtcagttgcg gccttgcgcg taaacaccgg atagtcgctg tacccctgca cctgcgcgag
    12781 cttgatctca gtatcccaat acgctccctt taccagccgc gtcggaattc tatgccctgt
    12841 gcggcgcccc agcgccgcca cccattcgat caacgggaac gcacgcttct gatacgcctg
    12901 cacggccagg cccaacccat cccagccagc aagccgagga tcgcccagca cagcggcaaa
    12961 gatgtcgaga ctgatctcca gcctatcagc ctcctcggca tccaccgtaa gcccgatgcc
    13021 agcctccttc gctgccaatg ccaggcctgc aagcgagccg atcagcgcag gcacacactg
    13081 caccgcctgc aatggctcat agcgcggatg caacgccgaa agcttcaccg atatcccggc
    13141 attcgcgccc ttgatcacgc tgatcgcgtg cagatacgcc tgttgatacc gctcggcatc
    13201 ggcatgagtc ttcgcggcct cgcccagcat atcatagctg tatcgcagct tccgattggc
    13261 tcgccccacc gcctcctcaa tcgtgcgacc catgacgaac tggcgcgcca gcatccgcat
    13321 cccacggcga agcgcggtac ggatcaccgg ctcaccaagc cggcggatca tcgccccgat
    13381 cacacccgcc acattgtcca ggttcagcac gcgcccagtc agcatgaatg cccaggccga
    13441 cgcgttcacc gccagcgaat ccccctgccc gaggtgcttc tgccagtccg ctgccgcgat
    13501 gcggtcctcg atcagcgcat cggccgtcgc cgcatccgga atccgcaaca atgcctcggc
    13561 gaggcaaagc agagcgacac cttccgctgt cccaagccca tactcagcaa gaaagtcggt
    13621 gacatcggct cccggcctgt gcgatgcccg cgcggcattg acgagcgtac gagccctcac
    13681 cgcgacgcca ggcgacgacg cggcaatcgt cagcagcggc tccaatacat ccacctctgg
    13741 cgcacgatgc gaccggcgaa gtccgcccct gtcatccatt gtcattgcga gcggagcgaa
    13801 gcaatcctgc ccccacagcg caggttgaat tcagatgaac tgcgccgcgt cgaacccgtc
    13861 cggcaaattc cccgttggct tgatcagatc atatggcgcg cgtgccagga actgcccggt
    13921 tccgggctcc gcctgtacct tcccatcgcg catcgccacc cgtccgcctt tcaccgtggc
    13981 aaccggccag ccggttactt ccatcccctc gtacggtgta tagtctattg tatgctgcat
    14041 caactcattg gtgatggtga cgcgcttccc cgcatcccac agcaccaggt cggcatccgc
    14101 tcctggcgcg atagtccctt tccgaggata cagcccgaac agcttcgccg gattggtaga
    14161 aatcagccgc acataagtgt tgagatcgat ccgccccttc gcaacgccct cgctgaacac
    14221 cagaggcagc cgtgccgcca accccggcac accattggga atatcaggga aaggcgcatt
    14281 gttgccatga atacgcttgc cgccttcgcc ttcgtagctg gcgccgctgt gatcggatga
    14341 taccacatcc agcgtcccgc gcttcacatc gttccacagt gcggcacttg tcgcggcgtc
    14401 gcgcggactg gggctgcaca tgaatttcgc cccctcgaag ccaggccgat ccatatccgc
    14461 cgcaccgagc acgaaatatt gcgggcacgt ctccgcccac accttcaccc ctcgcgcctg
    14521 cgcccgggca atctcctcgg ccacttcgga gcacgacacg tggaacacct ggatcggctg
    14581 gtccaccatc tcggccaacg caacagctcg ataggtagcc tcgcgctcca caaccttcgg
    14641 cctcgaccat gaatgatact tcggcgcgct tcgcccatcc gccatcaacg cggaggtccg
    14701 ccagcggatc gcctcataat tttcgcaatg taccgtcacc agcgctccgg tccgccgcgc
    14761 agccgccaat acacggatat attcgcgatc atccaaatgg gaaggttcgt aggtcaggaa
    14821 caccttcagg ctccgcaccc ctgacgccac gacctgtgga atctcgtcgc gcacaacgac
    14881 atcggacgga tcggtgatga tctggtgaaa actataatcc accatcgcct tcttcgcccg
    14941 ccgatggtat tcggcgagcg tatccaggat gccatgcccc ttgaactgcg cggcaaaggt
    15001 gatgaccgtg gttgtcccgc cagccagaca agcggcgcta ccggtgacaa acgtctcctc
    15061 gtcagaaccg ccattcggcc gcagctgctc gatgtggcaa tgcgtatcga cgccgccggg
    15121 catgacatac agcccgcccg catcgagcgt gtcagtgcca tccagcttat caccgaccgc
    15181 cgcgatacgc ccgccgcgta tagcgacatc ggcgcgatac gtatcagccg ccgtcaccac
    15241 ggtaccgccg cggatcacca gatcatagtc ggccatgtgt cttgcctccg gtcagatacg
    15301 tgaactcggc cgatagaatg gcgcatgttc ggccgggtcg aaatagttgg cgtagaacgc
    15361 cttcacatgt ggcagcagcg ccttcgacag tttccgccgc tccatctcgt catccgcgag
    15421 cgcacgctgc aaatcctgat gcaattcgcg caaggccgca tcgcgatcga ccttggtgaa
    15481 ccgtccctcg tcgtagatca cctcgccgtc gcagatcacc gtgcgagcac tcttcgcccg
    15541 ctgcagcacc gcatccagcg tcggcgtctc ctcatccaga tacggccagc tcagcgagcg
    15601 ccagtcgatc aacaccacat ctgccgcgcg ccccggctcg agcgcgccaa tccgctcgcc
    15661 gaacgccgtg gtcttggcgc cgccgaccgt ggccatccga aacacctgcg ccgctgtcgg
    15721 caccacatcg tcctccatgc ccggcacacg gtgcgcccgc agcaccatcc gcatttcttg
    15781 cagcatgtcg cgatcgtcgt tgatgcccgc ttcgtcgatg ccgatagcgg tgttgatgcc
    15841 cttcgcctca aacgcattca gcgctgccac acccgaccgc aggcggaaat tcgacgaaca
    15901 gttatggcac acgcaggtgc cggtctccgc cagccgatcg atgtccttct cgttcagcca
    15961 tacaccatgc cccaacgtca tgtgaggccc cagcagcccg aaccggtcga tatactccac
    16021 cgcagtgcca ccaccgcgcc ggcgcgcgta ctgcttctga tacgcggttt ccaccagatg
    16081 catgtgcatc ggcacttcgt acttgcgcga ggtctcggca aatcgcgtca acgccgcatc
    16141 agagcaccaa tgcagattgg ccggcgacaa ctggaccttc acacggcgat cattgtggtg
    16201 ccggccatgg agatcctcga acacggcgaa aaagtcatcc agagacaact ggaaccgatc
    16261 gaaccatcgc tgcaccggcc cgcgcagctc catcggcagg ctagtcacga acgccgcatc
    16321 atcctgatac acgagccggt tctgatcccg caccgagaac gaataggaca cccgcatccc
    16381 cacgtcctga taagcgcgca gtatctcctc acaccgcgcc tccacctccg gcaaccctcc
    16441 gggtgcccaa ccgtgcagat gctgcaccgt ggtgatcccc gacgcaatca tctcgaatgc
    16501 ggaatacagc gtatccaggt acggattgac attccgcatc accatacggg tcacaaacca
    16561 cagttccagc ggcatatccg gtgagccgag ctgcaccggc gtcagcccga catggtgatg
    16621 cccattgatg aaacccggca gcaggatatc gtcgcctgac ccaatcaccg gcacggtcgg
    16681 atgcaaccgc gacagatcgg cgaacgtgcc gacagcggtg atcatgccat cttcctgcag
    16741 caccgcaccg tctgcgatct cctcccagcg gtagcgatcg agagcgcgcg tgatcatcgc
    16801 gcggctgcgg atcagagatg cggccatgtg tctcgcccca ggctgtcttc tccggcatgg
    16861 tggcgcgcca tgcgcctgtc ccacaagacg attcgcccca ccggccgccc gatcaccatc
    16921 cagttcgatg gccgcccgat cccggcactc gaaggcgaaa cgatcgccgc ggcgctttcc
    16981 gccgccggaa tcgtcgcgtt ccgccggacg ctaaccggcg ccccgcgagg tctgcactgc
    17041 ggcatgggcg cgtgcttcga ttgcgttgta tcggtcgatg gccgcatcgg ccagcgcgcc
    17101 tgcatgacca aggccgccga cggaatgatc gtgtccggcg agccaacgac acccttggcc
    17161 ccgctggcac ctgaacccga agccggagaa tcgccggaac agggctgcga cgtgctggtg
    17221 attggcggcg gacccgccgg cctgtccgcc gccatcgcgg ccgcggaatc cggcgcatcg
    17281 gtcgtggtgc tggacgaacg caccgccacc ggcggccagt acgccaagcc gcttgccgac
    17341 agccacgtcg acgccgctcc agacgcgcag ttccggctcg gcatcgatct ccgcgatcaa
    17401 gcgctcgccg ccggcgcgcg catcgaaacg gacgccacgg tctggggagg ctttgccccc
    17461 gacgaaattg ccgcgttggt gcaaggccgc gccatcacct atcgcccgcg ccgcctgatc
    17521 atcgccccgg gtgcgcacga acgccccgtg cccctgccag gctggactct gccaggcgtc
    17581 atgaccacgg gttcgctgca aacgctggtg cgcgcccaac gtgtctgccc tggcgagcat
    17641 gtgctgatcg ccggcagcgg cccgctcaac ctccagcttg cctgtgaact cctggcctgc
    17701 ggcgtgaagc cgctcgccgt cgcggaatcg gctcctcgcc ccagtgcctc cgcgtggcgg
    17761 caggcctgga ggatggcccg cactgcgcca gatctactgc gcgagggcat cggcatgctg
    17821 gtaacgctga agcgcgccgg cgtgcccgtc ctctggtctg cccggatcaa gcagctcacc
    17881 ggggacggcc gcgtccagac tgcacatgtc gctggtcgca ccttcgatcg tagctacaac
    17941 gtggatgtcg tcgcgctaaa cctcggcttc c
//
LOCUS       DATGEM010000239         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_290615, whole genome shotgun sequence.
ACCESSION   DATGEM010000239 DATGEM010000000
VERSION     DATGEM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290615"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2497)
                     /locus_tag="VKQ27_07575"
     CDS             complement(<1..2497)
                     /locus_tag="VKQ27_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY88828.1"
                     /translation="MLGARAVFQLPVSQYPKTENAVVTVSTTYYGADAQTIAGFITQP
                     LEAAISQAQGIDYLSSNSIAGVSIITATLRLNYDANRAMTEINTQVASVRNQLPPAAQ
                     QPVITVQVGQQVDAMYLGYFSDTIPPNSTTDYLLRVVKPKLDSIDGVQTAEILGGRTF
                     ALRAWLDPQRMAARGVSADEVYAALGSNNYLAGVGSTKGQMVSVDLKTNTDVQSLDEF
                     RQLVIKRQNGALVRLEDVANVVLGAENYDFNVAFSGQRSVFIGIKVAPRANILDVAGR
                     VRAALPDIAAQLPTGVTQKLAYDATIFVTSSIKEVIKTLVQALIIVTVVIFLFLGSPR
                     AVVIPVVAMPLSLIGAFFIMQSLDYSINLLTLLALVLAIGLVVDDAIIVVENVDRHLK
                     EGVPPFKAAIQAARELGGPIIAMTIVLVAAYVPIGLQGGLTGALFTQFAFTLAGAVTV
                     SAVIALSLSPMMCAKVFRSEQEEGRFTHFLDRTFNRLRTAYVRRLSNWLTTWPAVVLF
                     GALLLPCVALLFMTSKSELAPQEDQGVVLEATTGPPDATLNQMQAYSDAEFQIAKKLP
                     EYRQMFQLTGQPTVNQGFGGVLFVPWEQRHRSAAELQMLLQSEWNKIPGARVAAFQFP
                     ALPGAFGLPIQFVITTTEPIENLNEVANQVLDKARASGKFWFIDSDLKLDKPQQTVTV
                     DRDMVSDLGLTQQNVGSSLAAALGGGYVNYFSIAGRSYKVIPQVLQVDRLNPDQVLDY
                     YVRAGDGSLMPARTVANIKTTTVPESINHFQQLNSATIAGAPAVSQDQALKFLRDALK
                     EAAPSGYTADYSGESRQFMLGSGSFTLTLLFAII"
     gene            complement(2576..3679)
                     /locus_tag="VKQ27_07580"
     CDS             complement(2576..3679)
                     /locus_tag="VKQ27_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY88829.1"
                     /translation="MIIAAVAVVVLIVAIAGIKAILIMRMIAQAKANGAPAQTVTAAT
                     AAYSDWQPEISAVGSVRAMRGVDVTTEVTGLVRSVDFHSGDDAKRGQALVHLNADADI
                     ATLHSLEASADLASTVYARDKMQFEAQAISQAQLDADAADLRNKRAQAAAQAALVEKK
                     TLRAPFDGRLGITTVNPGQYLNTGDKVVTLQQLDPVYVDFRLPQEQLSQVHPGLEVRL
                     TLDAFPGETFAGRINAVDPLVDTSSRNFQAEATVPNPAHKVLPGMFVRVAVLASSKQR
                     YLTLPQTSITFNPYGSTVFLVQKDEKGKRTAQQTFVTTGPTRGDQIAVLKGIKEGDEV
                     ITSGQLKLKNGTPIDISSALQPSDAANPTPQEH"
     gene            complement(3700..>4036)
                     /locus_tag="VKQ27_07585"
     CDS             complement(3700..>4036)
                     /locus_tag="VKQ27_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLY88830.1"
                     /translation="LERATAVYREAVLSALQDVADTLRALEADARTLQADLITETDAQ
                     DTLNMTKLQYKVGGVSYLQLLNAERVFLQARQNRVQAEAARYADTAALFQALGGGWWN
                     RDNGKESRW"
BASE COUNT          686 a         1261 c         1356 g          733 t
ORIGIN      
        1 cgataatcgc gaagagcagt gtcagggtaa agctgcccga accgagcatg aactgccggg
       61 actccccgga gtaatcggcg gtgtaccccg aaggcgcggc ctccttgagc gcatcacgca
      121 ggaacttcag cgcctggtcc tgggagaccg cgggagcccc ggcgatggtt gccgagttga
      181 gctgttggaa atggttgatc gattccggca ccgtggtcgt cttgatgttc gcgaccgtgc
      241 gcgccggcat gagcgagcca tcgcccgcac gcacgtagta gtcgagcacc tggtcgggat
      301 tcaaccgatc gacctgcagc acctgcggga tgaccttgta ggagcggccc gcgatcgaga
      361 agtaattgac gtagccgccg ccgagcgcgg cggcgagcga gctgccgacg ttctgctgcg
      421 tgaggccgag gtcactcacc atgtcgcgat cgacagtcac ggtctgctgc ggcttgtcga
      481 gcttcaggtc cgagtcgatg aaccagaact tgccgctggc gcgcgccttg tcgagcacct
      541 gatttgcaac ctcgttcagg ttctcgatcg gctcggtggt ggtgatgacg aactggatgg
      601 gcaggccgaa cgcgccgggc agggcgggga actggaatgc ggcgacgcgt gcgcccggga
      661 ttttgttcca ctcgctctgc agcagcatct gcaactcagc cgcgctgcga tgccgctgct
      721 cccagggaac gaacagcacg cccccgaagc cctgattcac ggtgggctga ccggtcagct
      781 ggaacatctg ccggtattcg gggagcttct tggcgatctg gaactcggca tccgaatacg
      841 cctgcatctg gttcagcgtc gcatccggcg gacctgtagt cgcttccagt accacgccct
      901 ggtcctcctg cggcgcaagc tctgacttcg acgtcatgaa caggagagct acacaaggaa
      961 gcagcagtgc gccgaacaac accaccgctg gccaggtcgt cagccagttg gacaggcgcc
     1021 gtacgtaggc ggtgcgcagc cggttgaagg tgcggtcgag gaagtgggtg aaacggcctt
     1081 cctcctgctc gctgcggaag accttcgcgc acatcatggg cgaaaggctg agcgcgatca
     1141 ccgccgagac ggtcacggcg cccgcgagcg tgaaggcgaa ctgggtgaac agcgctcccg
     1201 tgagcccgcc ttggaggccg atcggcacgt aggcggcgac cagcacgatg gtcatggcaa
     1261 tgatcgggcc accgagctcg cgtgcggcct ggatcgccgc cttgaagggc ggcactcctt
     1321 ccttgaggtg tcggtcgacg ttctcgacca cgatgatcgc gtcgtcgacc accagtccga
     1381 tcgcgagcac cagcgcgagc agcgtgagca ggttgatcga gtaatcaagc gactgcatga
     1441 tgaagaaggc gccaatcagc gacagcggca tggccacgac cgggatcacg accgcacggg
     1501 ggctgccgag gaacaggaat atcaccacgg tgacgatgat cagtgcctgg acgagagtct
     1561 tgatgacttc cttgatggaa ctggtgacga agatggtggc atcgtaggcc agcttctgcg
     1621 tcacgccggt gggcagctga gccgcgatat cgggtagcgc ggcgcgcacc cgtcccgcga
     1681 catcgagtat gttggcgcgg ggcgccacct tgatgccgat gaacaccgac cgctggccgc
     1741 tgaaggcgac gttgaagtcg tagttctccg cgcccagcac cacgttggcg acgtcctcga
     1801 gccgcacgag cgcgccgttc tgccgcttga tgaccagctg tcggaactca tcgagcgact
     1861 ggacgtcggt gttcgtcttc aggtcgacgc tcaccatctg ccccttggtg gaacccacgc
     1921 cggcgaggta gttgttggag ccgagtgccg cgtacacctc gtcggcagag acgccgcgtg
     1981 ccgccatccg ctgcgggtcg agccaggcgc gcaatgcgaa ggtacggccg ccaaggatct
     2041 cagcggtttg cacgccgtcg attgagtcaa gcttgggctt caccacccgc aacaagtagt
     2101 cggtggtgct gttgggcggg atggtgtcgc tgaaatagcc caggtacatg gcatcgacct
     2161 gctggccgac ctgcaccgtg atgaccggct gctgcgctgc gggcggcagc tggttgcgga
     2221 ccgaggcgac ctgtgtattg atctcggtca tcgcgcggtt ggcgtcgtag ttcagtcgca
     2281 gcgttgccgt gatgatcgag accccggcga tgctgttcga ggacaggtag tcaatgccct
     2341 gggcctggga gatcgcagct tcgagcggct gagtgatgaa gcccgcgatg gtctgggcgt
     2401 cggcgccgta gtaggtggtg ctgaccgtga ccacggcgtt ctcggtcttc gggtactggc
     2461 tcaccggcag ctggaacacg gcgcgcgcgc ccagcaccag gatcagcaga ctcacgacga
     2521 ccgagaggac cggacgctcg atgaagaggt cagtgaattt catcgccgcg ctccttcaat
     2581 gctcctgcgg agtcgggttt gcggcatccg aaggctgcag ggcgctgctg atgtcgatcg
     2641 gcgtaccgtt cttgagcttg agctgaccgc tggtgatgac ctcgtcgccc tccttgatcc
     2701 ccttgaggac cgcgatctgg tcgccacgcg tcgggcctgt ggtgacgaag gtctgttgcg
     2761 ccgtgcgctt gcccttctcg tccttctgca ccaggaacac cgtcgatccg taagggttga
     2821 aggtaatcga ggtctgcggc agggtcaggt aacgctgctt ggagctggca agcaccgcaa
     2881 cgcgcacgaa catgcccggc agtaccttgt gtgcggggtt cggtacggtc gcctcggcct
     2941 ggaagttgcg gctcgaggtg tcgaccagcg gatcaaccgc gttgatcctg ccggcgaagg
     3001 tctcgccggg gaaggcatcg agggtcagac gcacctcgag gcccggatgc acctgcgaaa
     3061 gctgctcctg cggcagccgg aaatcgacgt acaccgggtc gagctgctgc agcgtcacca
     3121 ccttgtcgcc ggtattgagg tactgccccg ggttgacggt ggtgatcccg agccgtccgt
     3181 cgaagggcgc gcgcagcgtc ttcttctcga ccaatgccgc ctgggccgcg gcctgcgccc
     3241 gcttgttcct gagatcggcc gcatccgcat cgagctgcgc ctggctgatc gcctgcgcct
     3301 cgaactgcat tttgtcgcgg gcgtagacgg tggaggcgag atcggccgag gcctccagcg
     3361 aatgcagtgt ggcgatgtcg gcgtcggcgt tgaggtgcac cagcgcctgg ccgcgcttcg
     3421 catcatcgcc cgaatggaag tcgacgctgc gcaccagtcc cgtgacctcc gtcgtgacgt
     3481 cgacgccgcg catcgcgcgc acgctgccga ccgcgctgat ctccggctgc cagtcggagt
     3541 acgcggccgt cgctgcagtg acagtctgcg caggcgcacc gttggccttc gcctgagcaa
     3601 tcatccgcat gatcaggatg gctttgatgc ctgcgattgc gacgatgagg acgacgaccg
     3661 ccacggccgc aatgatcaag cgtcgcttca agaggggctt caccagcgac tctccttccc
     3721 attgtcccga ttccaccatc cgccgccgag cgcctggaag agcgccgcgg tgtccgcata
     3781 gcgcgccgcc tccgcctgca cgcggttctg ccgcgcctgc aggaacacgc gttccgcatt
     3841 cagcagctgc aggtagctga cgccgcccac cttgtattgg agcttggtca tgttgagagt
     3901 gtcctgcgcg tcggtctcgg tgatcaggtc cgcctggagc gtgcgggcat cggcctcgag
     3961 cgcccgcagc gtatcggcga cgtcctgcag tgccgacagc accgcctcgc gatacacggc
     4021 cgtggcgcgc tcgagg
//
LOCUS       DATGEM010000240         6377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1157476, whole genome shotgun sequence.
ACCESSION   DATGEM010000240 DATGEM010000000
VERSION     DATGEM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157476"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..993)
                     /locus_tag="VKQ27_07590"
     CDS             complement(55..993)
                     /locus_tag="VKQ27_07590"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HLY88831.1"
                     /translation="MAMIGGLIVQGLQMLLVLLLAPLLTGLVRKVKARLQRRRGPSLI
                     QPYRDLRRLLSKDVVLAENASWLFRVAPYVIFATTWVAAALVPTFATGLVFSWSGDLI
                     AITALLGSARFAQALAAMDIGTSFGGIGASREMMIASLAEPAMLMIVLSVALIVGSTQ
                     LSTIADHMAFANVGLRVSLGLALIAFVVVALAENARIPVDNPATHLELTMVHEAMVLE
                     YSGRHLAMIELAAALKLLLYISVIGCIFFPFGLAESYLVGLLCYLVKLTFAGVLLGVF
                     EMSIAKMRVFRVPNFLGAALMLGLLGVLLLFVSEGM"
     gene            complement(984..2984)
                     /gene="hyfB"
                     /locus_tag="VKQ27_07595"
     CDS             complement(984..2984)
                     /gene="hyfB"
                     /locus_tag="VKQ27_07595"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HLY88832.1"
                     /translation="MIVLPLLCVALQLCMAVAGACFASRPSGRMVVYGGSLVISAATL
                     LIALFSLAAAPSDTVLPLGLPWTGAHFHLDPLAAFFLAVVGLGSAGACLYALGYGRHE
                     HEPQRVLPFYPLFLAGLNLVVIAADAFSFLFAWELMSLASWALVMSHHREGDNAHAGY
                     VYIVMASFSGLALLLCFGVLAGASGDYSFAAIGATHLSPGVATLALVLALIGAGSKAG
                     VVPLHAWLPLAHPAAPSHVSALMSGVMTKVAVYGFIRIAFDLLGIPAWWSATIVLAVG
                     GITAVLGVLYALMEHDLKRLLAYHTVENIGIIFIGLGLAMAFRANALTSAAALGLTAA
                     LFHVFNHSVFKSLLFFGAGAVQTATGERDMDHLGGLIHRMPKTAFAFLIGCMAISALP
                     PLNGFVSEWLTFQAILLHPNLPQWGLKLAIPADGALLALSAALAAACFVKAFGITFLG
                     RPRTRTAADARETDRFSLQAMFAFAGLCLLAGILPGMVIDLLAPVTQALIGAHMPTQL
                     DVAWLSIIPVASARSSYNGLLVFVFIAASASLTVLAIHRFASHAVRRAPPWDCGFPDP
                     RPETQYTGGSFAQPIRRVYGATVFRTQETVTMPLPGELTAARIEKHLTDTVWAAFYAP
                     VATVIWRLAELLNVLQFLTIRRYLGFVFAALVILLLALALWQ"
     gene            complement(2981..3226)
                     /locus_tag="VKQ27_07600"
     CDS             complement(2981..3226)
                     /locus_tag="VKQ27_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY88833.1"
                     /translation="MRAARAMLGIDQRELARLSGLSLPTVQRMEASDGVVRGNVDSLM
                     KLVGALHEAGIELIGDNAASSQGGRGVRLTTPAPRRT"
     gene            complement(3331..3912)
                     /locus_tag="VKQ27_07605"
     CDS             complement(3331..3912)
                     /locus_tag="VKQ27_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88834.1"
                     /translation="MSVADPNHLILTGENPFLRLSETDSGTNFTTNASYWRIITCPAG
                     PGHVLYLKSELIGEHWRIYSDNIAMARWLQSTVQGMLNAELSDQSIPVTEASFYKEGD
                     PRYFWTEGVSAHDEEIALTWYSIGEPLLIHTQPNAEPGRPYGVCTVLIPALGGRLTRN
                     GVHAKGKPWPRERESRPFSTSALAFSESWTEAR"
     gene            complement(3930..4709)
                     /locus_tag="VKQ27_07610"
     CDS             complement(3930..4709)
                     /locus_tag="VKQ27_07610"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY88835.1"
                     /translation="MDLANQVALVTGAAHGIGKASACALAEAGAHVIVADIDRDGARA
                     TAESIAAGQRRAVAMQADVGDLNQIDRMVREALAAFGRIDILLNNAGVTRRADIMDII
                     EADWDRIHRVNAKGVFFCLQRVAQEMIPQHSGRIINIASIAGKGYVGASNVAYAASKG
                     AVIGMTKLAALQLAKHNINVNAICPGVTRTALSNANLAVAAQQQGVTLEEMERRRADV
                     IPLKRANDPEDIAAMVVFLASSGARNVTGQSFNVDGGLIPD"
     gene            4847..5152
                     /locus_tag="VKQ27_07615"
     CDS             4847..5152
                     /locus_tag="VKQ27_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88836.1"
                     /translation="MSLFRRFLPVVSDAHMEAPDPQRPIPLYDPGGMMNRMNRSFRRR
                     LRDRVEDLFHDACVTGDLATAEELLGVLENMQKRRQATGQDRRAGDSPFTAARVVGC"
     gene            5220..5513
                     /locus_tag="VKQ27_07620"
     CDS             5220..5513
                     /locus_tag="VKQ27_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HLY88837.1"
                     /translation="MAVRLVVTISANPGKGGELAQVFRARCAECMQEPGCEQFEIFQS
                     VLNPDKLALLELWTDQAALDAHAKLNATRPPMPSGLRAGGGVREDYTYTVTRS"
     gene            5595..5816
                     /locus_tag="VKQ27_07625"
     CDS             5595..5816
                     /locus_tag="VKQ27_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88838.1"
                     /translation="MRRGFPVVLVLATLLGGCVGTNSAGGQAQNAAPIGYRYYDSQHP
                     NYVIQPSAEAIANAQRGTYLWPPAMSVVR"
     gene            complement(5820..>6377)
                     /locus_tag="VKQ27_07630"
     CDS             complement(5820..>6377)
                     /locus_tag="VKQ27_07630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88839.1"
                     /translation="RTANAFGGLDFVWNHVGHPGPAAIEGLDWKDFELAMDLNLRTVL
                     VTTEAAIPELRARGGGALLYTASTSGLTGSQFSPIYNIAKFGVVGLVHGLAKRYAREK
                     IRVNAVCPGPTDTPMLRVFVARPDSQLPPGETPETLVLKRGGQVPMGRPAQPEEVANA
                     ALFLISDEASYITGVALPVDGGATA"
BASE COUNT         1271 a         1965 c         2085 g         1056 t
ORIGIN      
        1 ccagcacgag tccgccggcc agcatgtgag agacatcgaa cgcgaaactg tgcgtcacat
       61 gccctccgag acgaacagca gcagcacacc gagcaggccc agcatcagcg cagcaccaag
      121 gaagttgggc acacggaaca cgcgcatctt ggcgatcgac atctcgaaca cgccgagcag
      181 cacacctgcg aacgtcagct tcaccaggta gcagagcagg cctaccagat agctctcggc
      241 cagaccgaat ggaaagaaga tgcagccgat gacggaaata tagagcagca gcttcaaggc
      301 agcggcgagt tcgatcatcg ccagatggcg accggagtat tccagcacca tcgcctcatg
      361 caccatcgtc agttccagat gggttgccgg attgtcgacc gggatgcggg cgttctccgc
      421 cagcgctacg acaacaaacg cgataagcgc gagccccagc gatacgcgca ggccgacatt
      481 ggcgaaggcc atgtggtcgg cgatggtgga aagctgcgtt gaccccacga tcagcgcgac
      541 cgacagcacg atcatcagca tcgcaggctc ggcgagcgag gcgatcatca tctcgcgtga
      601 ggcgccaatg ccgccaaagc tcgtgccgat atccatggca gcaagcgcct gtgcgaagcg
      661 ggcgctgccg agcagcgcgg tgatcgcaat caggtcgccg gaccagctga acacgaggcc
      721 agtggcgaac gtgggaacca atgccgccgc aacccaggta gtggcaaaga tcacataagg
      781 cgctacacgg aacagccagg aggcattctc ggccagcacc acgtccttgc tgagcaaccg
      841 ccgcaggtcg cgatagggct ggatcagcga gggcccccga cggcgctgca gacgcgcctt
      901 caccttccgc acaaggccgg tgagcagcgg tgccagcagc agcaccagca gcatctgcaa
      961 gccctggact atcaggcctc cgatcattgc catagcgcga gagccagcag caggattacc
     1021 agggcagcga aaacgaaacc gagatagcgc ctgatggtca ggaattgcag aacgttcagc
     1081 agttccgcta ggcgccagat gacggtggca acgggcgcgt agaacgccgc ccacaccgta
     1141 tcggtgaggt gcttctcgat gcgggcggcc gtcagttcgc cgggcagcgg catcgtgacc
     1201 gtttcttgcg tgcggaacac cgtggcgccg tatacacggc ggattggctg ggcgaagctg
     1261 ccgccggtgt attgcgtctc gggccgcgga tcggggaagc cacaatccca gggcggtgca
     1321 cggcgcaccg cgtgtgaggc gaagcgatga atcgcgagca cagtcagcga tgccgaggcc
     1381 gcgatgaata cgaacaccag cagcccgttg taggaactgc gcgcgctcgc gaccgggatg
     1441 atcgacagcc atgcaacgtc gagctgtgtc ggcatatgcg cgccaatcag tgcctgcgtg
     1501 accggcgcca gcaggtcgat caccataccg ggaaggatcc cggcaagcag gcaaagcccg
     1561 gcgaaggcaa acatcgcctg cagcgagaaa cgatcggtct cgcgcgcatc ggcggcggtg
     1621 cgcgtgcggg ggcggccaag gaaggtgatg ccgaacgcct tgacgaagca tgccgctgca
     1681 agcgcggcgg acagcgccag cagcgcgcca tcggcgggaa ttgcgagctt caggccccat
     1741 tgcggcaggt tgggatgcag caggatcgcc tggaatgtca gccactcgga aacaaacccg
     1801 ttcagcggcg gcagcgcaga gatcgccatg cagccgatca ggaaggcaaa cgcggttttc
     1861 ggcatgcgat ggatcagccc gccgaggtga tccatgtcgc gctcaccggt tgcggtctgc
     1921 acggcgcctg cgccaaagaa cagcaggctc ttgaacaccg agtgattgaa cacgtggaac
     1981 aacgctgccg tcagcccaag tgccgcggcc gaggtaagtg cgttggcacg gaaggccatc
     2041 gccagcccaa ggccaatgaa gatgatgccg atattctcca ccgtgtggta ggcgagcagg
     2101 cgcttcaggt cgtgttccat gagcgcgtaa agcacgccaa gcacggcggt gatgccgccg
     2161 acggcaagga caatcgtggc agaccaccat gccgggatgc cgagcagatc gaaggcgatg
     2221 cggatgaagc cgtacaccgc gaccttggtc atcacgccgc tcataagcgc ggagacgtgg
     2281 ctcggcgccg caggatgggc gagcggcagc caggcgtgca gcggcacaac gcccgccttt
     2341 gatccggcgc cgatcagggc cagcacgagc gccagggttg caacgcccgg ggataagtgc
     2401 gtggcgccga tcgcggcaaa gctgtagtcg cccgacgcgc ccgccagcac gccgaaacag
     2461 agcagcaggg cgaggcccga gaaactcgcc atgacgatgt agacatatcc ggcgtgggcg
     2521 ttgtcgccct cccggtgatg cgacatcacc agtgcccagg aggcgagcga catcaactcc
     2581 catgcgaaca gaaagctgaa tgcatcggcg gcaatgacga cgaggttcag gccagcgaga
     2641 aacagcggat agaacgggag cacgcgttgt ggttcgtgct catgacggcc atagccgagc
     2701 gcgtagagac aggcgcccgc actgccaaga ccaaccaccg ctaggaagaa agcagccaac
     2761 gggtccagat gaaaatgcgc gccggtccag ggcaggccaa gcggcagcac cgtgtcggac
     2821 ggcgcggcgg caagcgagaa cagcgcgatc agcagcgtgg ccgcggagat cacgagggag
     2881 ccgccataga cgaccatgcg gcctgatggt cgtgaggcaa agcaggcgcc agcgacggcc
     2941 atgcagagct ggagcgcgac gcatagcaga ggcaacacga tcatgttcgc cgtggcgccg
     3001 gcgtcgtgag gcgcacgcct cggccaccct ggctgctggc agcgttatcg ccgatcagtt
     3061 cgatgccggc ttcatggagc gctccgacca gcttcatcag cgagtcgacg ttcccgcgca
     3121 cgacgccatc actggcttcc atgcgctgaa cggtcggcag cgagagaccg gaaagcctgg
     3181 ccagctcgcg ctgatcgata cccagcatgg cgcgcgcggc gcgcatctgt gtgccagtga
     3241 tcataggcgg gcttgcagat atgatgtttc aggcatcagt attaagactg acacctttgt
     3301 aatcactccg caatccttga aaccgataga ttaacgcgct tctgtccagc tctccgaaaa
     3361 cgccagcgcg ctggtgctga acgggcggct ttcgcgctcg cgcggccatg gcttgccctt
     3421 cgcgtgaacg ccgttccgcg tcagcctgcc gccgagggcg gggatcagga cggtgcacac
     3481 gccgtacgga cgacccggtt cggcgttcgg ctgggtgtgg atcagcagcg gttcgccgat
     3541 gctgtaccag gtcagcgcga tctcctcgtc gtgcgccgaa actccctcgg tccagaaata
     3601 ccgcgggtcg ccttccttgt agaacgatgc ctcggtcacc gggatggact gatcagagag
     3661 ctccgcattc agcattccct gcacggtcga ttgcagccag cgcgccatcg cgatgttgtc
     3721 cgagtaaatg cgccaatgct cgccgataag ctcgctcttg agatacagca cgtggccagg
     3781 tccggcgggg caggtgatga tgcgccagta gctggcattg gtggtgaagt tggttccgct
     3841 gtcggtttcg ctgagccgaa ggaatggatt ttccccagtc aggatcaggt gattcgggtc
     3901 ggcgacgctc atggttgtgc ctcctcggtt caatcgggaa tcaggccgcc atcgacgttg
     3961 aacgactgcc cggtgacgtt gcgcgcgcct gacgacgcca ggaacactac catggcggcg
     4021 atgtcctcgg gatcgttggc gcgtttcaaa gggatgacat ccgcgcggcg tcgctccatt
     4081 tcctccagcg tcacgccttg ctgctgcgcg gcgaccgcaa ggttggcgtt ggacagcgcg
     4141 gtgcgcgtca cgccaggaca gatcgcattg acgttgatgt tgtgcttcgc cagttgcagc
     4201 gcagccagct tcgtcatgcc gatcactgca cccttgctcg cggcgtaggc gacgttggat
     4261 gcgccgacat agcctttgcc ggcgatcgag gcgatgttga tgatgcgtcc gctgtgttgc
     4321 ggaatcatct cctgtgcgac gcgttgcagg cagaagaaca cgcccttcgc gttgacgcgg
     4381 tggatgcgat cccagtccgc ctcgatgatg tccatgatgt cggcgcggcg cgtgacaccg
     4441 gcattattca gcaggatgtc gatgcggcca aacgccgcaa gcgcttcacg caccatgcgg
     4501 tcgatctggt tcaggtcacc gacatcggct tgcatcgcaa cggcgcggcg ctggcccgcg
     4561 gcgatggatt cggcagtcgc tcgggcgccg tcgcggtcta tgtcggcgac gatgacgtgc
     4621 gcgcctgcct cggcgagcgc gcaggcggat gccttgccga ttccgtgcgc ggcaccggta
     4681 acgagtgcca cctggtttgc gaggtccata tggcgtttcc tcctgttcga tcgcagtgaa
     4741 gccgtgattg gcggcggcgt ccactggaag cactctcgtc ttcgtcattc gccgcaatgg
     4801 aatctgctgg ccagatgttc gtgggcagca gtattaataa cgcggcatgt cactctttcg
     4861 gcgattcctg ccagttgtat ccgacgcgca tatggaggcg cctgatccgc agcgcccgat
     4921 tccgctctac gaccctggcg gcatgatgaa ccgcatgaac cgctcgttcc ggcgccgcct
     4981 tcgtgatcgg gtcgaagatc tgttccatga cgcatgcgtc accggagacc tcgccaccgc
     5041 cgaggaattg ctgggcgttc tggagaatat gcagaagcga cggcaagcta ccggacagga
     5101 ccggcgtgcc ggggactccc cctttaccgc ggcacgtgtt gtcggctgtt aggctggttg
     5161 cctgacaatc gcaaaacagg gacacttcca cgcaatccgt tcacagggga ggaagtggca
     5221 tggcggtccg tctcgtggtg accatatcgg caaatccagg caagggcggc gaactcgccc
     5281 aggtgttccg agcgcgctgc gccgagtgca tgcaggaacc gggctgcgag cagttcgaga
     5341 ttttccagag cgtgctcaat ccggacaagt tggcgttgct ggaactatgg acagaccagg
     5401 ccgccctgga tgcacacgcc aagctcaatg cgacgcggcc tccgatgccg tccgggctac
     5461 gagcgggtgg cggcgtgcgt gaggactata cttacactgt gacccgatcg taactgcttg
     5521 gggttgcaag gctcgcgttg caactttgcc gcgacgcacc aactacccgc catcgtgcta
     5581 cggagtacct ggcgatgcgt cgcggctttc ctgtcgtact tgtcttggcc acgctgcttg
     5641 gcggctgcgt cggcacaaat tccgcgggcg gccaggcgca gaatgctgcg cccataggct
     5701 accgctatta tgacagccag caccccaact atgtgattca gccatcggcc gaggcgatcg
     5761 ccaacgcaca gcgtggcacg tacctttggc cgccggcgat gagcgttgtc cgctaggggt
     5821 tacgccgtgg cgccaccgtc caccggtagc gcgactccgg tgatgtagga ggcctcatcc
     5881 gagatcagga acagcgccgc gttggcgacc tcctcgggtt gggccgggcg tcccatcggt
     5941 acctggccgc cgcgtttcaa caccagcgtt tccggtgtct cgccgggcgg caactggctg
     6001 tccgggcgag caacaaacac gcgcagcatc ggcgtatcgg ttgggccagg acagactgcg
     6061 ttgaccctga tcttctcgcg cgcgtatcgc ttggcgaggc cgtgcacaag gccgacgacg
     6121 ccgaacttgg cgatgttgta gatcgggctg aactgcgagc cggtcagacc ggatgtggac
     6181 gcagtgtaga gcagcgcgcc gccgccgcgg gcgcgcagtt ccggaatggc agcctcggtg
     6241 gtgacaagaa ccgttcgcag gttcaggtcc atggcgagtt cgaaatcctt ccagtcgagg
     6301 ccttcgatag cggcagggcc cggatggccg acatgattcc agacaaaatc gagcccgccg
     6361 aaagcgttcg ctgtgcg
//
LOCUS       DATGEM010000241         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1043974, whole genome shotgun sequence.
ACCESSION   DATGEM010000241 DATGEM010000000
VERSION     DATGEM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043974"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VKQ27_07635"
     CDS             complement(<1..824)
                     /locus_tag="VKQ27_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HLY88840.1"
                     /translation="MLALLPPPSGLTEHAWLYFAVFAGVIAALVTEPLPNPAVGLIGV
                     ALMGVLSRYTLYAPADLAKPGFNVTSQTISWVLSGFASTTVWLVGAAFMFALGYQKTG
                     LGRRIALLLVRALGKSTLRVGYATALADAILAPFTPSNTARGAGITFPIVSNLPALYD
                     SRPNDPSARKFGGYIMWTTFAANCITSSLFMTACAPNFLAIEFIRRIVHVDITYVQWM
                     KGSLPFALPLLLALPLLVYWLYPPEVKRSPEVSVWAGGELRKMGAISKNEIILSVLV"
     gene            1069..2205
                     /locus_tag="VKQ27_07640"
     CDS             1069..2205
                     /locus_tag="VKQ27_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLY88841.1"
                     /translation="MAMNERTLAVITPEGLGGLIDGLRTDGFRVIGPKLRDGAIIYDD
                     IETLSDLPAGWTDEQDGGHYRVRRRDDAALFGYNVGPHAWKRFLHPPLLRLWRAERSA
                     DGMAIADTSDTAERYAFLAVRSCELHAIGIQDRVFLQGPYVDPHYRARREGAFIIAVN
                     CGDAGGTCFCTSMKTGPKAQDGFDLSVTELLADEHEFLVEVGSKRGAALLATVPNRPP
                     DARHTTMAADATARAEGNQGRHMRSDDVRELLQANLEHPRWDDVAQRCLTCGNCTMVC
                     PTCFCTSVFDENDLATGAAERKRRWDSCFTMDFSYIHGGSVRSSARSRYRQWMTHKLA
                     NWVDQFGTSGCVGCGRCITWCPVGIDITEEVRAIRGEGGSDGRA"
     gene            2192..2653
                     /locus_tag="VKQ27_07645"
     CDS             2192..2653
                     /locus_tag="VKQ27_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLY88842.1"
                     /translation="MEGLERIVAEHKLFEGLGPEFISLAAGCAKNVRFDTDQYLFHAD
                     DPADWIYLVRFGRVALEMVTPGRGAVQYETLGPGEVVGLTWLLPPYRWGYDARAIELV
                     RAIALDAKCLRDKCEVDHNLGYEVLKRFLPVLVQRLQSVRLQMLDVYGSAR"
     gene            2637..3491
                     /locus_tag="VKQ27_07650"
     CDS             2637..3491
                     /locus_tag="VKQ27_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HLY88843.1"
                     /translation="MDRLAERAPVTDAMVPRIARVARRARDLPDTWTLDLVSPDGGPL
                     MHYAPGQFTMMYVFGVGEIPVSISGDASDATRLVQTVRAVGKVSEAVTRLSAGDTLGI
                     RGPYGTSWPVEEGYGRDVVVVAGGLGLAPLRPALYQLRRERKRFGKLVLLYGTRRPED
                     ILYRRQLETWRRQLDMEIEVTVDHANTEWRGNVGVVTKLIPRLGIDTANAMALVCGPE
                     IMMRFAASALHDVGMPEEAIHLSMERNMKCAVGLCGHCQFAGSFICKDGAVMRYDRIR
                     RLLSLKEI"
     gene            3611..4387
                     /locus_tag="VKQ27_07655"
     CDS             3611..4387
                     /locus_tag="VKQ27_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HLY88844.1"
                     /translation="MNAGRRPKLAVWKFASCDGCQLSLLDCEDELLAVAGAVEIAFFP
                     EATRAVVRGPYDVSLVEGSVTTPHDAERLHTVRRQSRTLITIGACATAGGIQALRNFK
                     DVREFTQVVYARPDFIDTLTTSTPIKAHVNVDFELHGCPINKHQLVEVLAAFLNGRQP
                     NAPAHSVCVECKLRGTACVLVRGIPCLGPVTHAGCGAICPAYNRGCYGCFGPMETPNA
                     PALSRKLEACGMGQRDLQRVYSTFNANELPFREESARHGS"
     gene            4377..5663
                     /locus_tag="VKQ27_07660"
     CDS             4377..5663
                     /locus_tag="VKQ27_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HLY88845.1"
                     /translation="MARKTINVDYLARVEGEGGVKITFDGNTVRDVQLRIFEPPRFFE
                     AFLRGRAYTEAPDITARICGICPVAYQMSAVHAMEDALGVTVTPEVRELRRLLYCGEW
                     IESHVLHIYMLHAPDFLGYPGAVEMAKDHGDLVRQGLELKKIGNEILEVLGGRAIHPV
                     NVRVGGFYRAPTRREFAPLAERLKHARDIARATVRWVAGFDFPDCERDYEYVSLGAAG
                     EYPFNAGHVVSNKGLDITPHQYDAYFEESHVKRSNALHSHLQGRPYLTGPNARYSLNA
                     AQLSPLAREAAQEAALGETCTNPFRSIVVRAVEVLYACDEALRIIDAYREPEQPHLDL
                     TPRAGVGYACTEAPRGMLYHRYRIDADGSILDAKIVPPTSQNQPSIEDDLKTFAGDWL
                     DLPEEALRVRCEQAVRNHDPCISCATHFLKLEVVRM"
     gene            5660..>5848
                     /locus_tag="VKQ27_07665"
     CDS             5660..>5848
                     /locus_tag="VKQ27_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88846.1"
                     /translation="MTGRVVLAIGNPDRGDDAVGRFVGSLLQSSVMPDIPVTLLNGEG
                     AGIVAALEGVDEAWLVDAA"
BASE COUNT         1039 a         1695 c         1997 g         1117 t
ORIGIN      
        1 acgaggaccg aaaggatgat ctcgttcttc gagatcgcgc ccattttccg cagttcgccg
       61 cctgcccata cagacacctc cgggctgcgc ttcacctcgg gcggatacag ccaatagacc
      121 agcagcggca gcgccagcag caacggcagc gcgaatggca gcgagccctt catccactgc
      181 acatacgtga tgtccacatg cacgattctg cggatgaact cgatcgccag gaaattcggc
      241 gcgcacgccg tcatgaacaa cgaactcgtg atgcagttcg cggcgaacgt cgtccacatg
      301 atgtagccgc cgaatttccg cgccgacgga tcgttcggtc gcgagtcata gagcgcgggc
      361 aggttgctca cgatcgggaa ggtgatgcct gcgccgcgcg ccgtgttcga cggtgtgaac
      421 ggcgccagga ttgcgtccgc caatgccgtc gcgtaaccga cgcgcagcgt gctcttgccg
      481 agtgctcgca caagcagcag cgcgatgcgc cggccgaggc cggtcttctg gtagcccagc
      541 gcgaacatga acgcggcgcc gacgagccac accgtggtgc tggcaaagcc gctcaacacc
      601 cagctgattg tctggctggt gacgttgaag cctggctttg ccaggtcagc cggcgcgtag
      661 agtgtgtaac gcgacagcac gcccatcagc gccacgccga tcaggccaac cgcagggttc
      721 ggcagcggtt ctgtcacgag cgcagcgatg acaccggcaa ataccgcgaa atacagccag
      781 gcgtgctcgg tcaatccgga tggcggcggc agaagcgcaa gcattactgt gatcgccaca
      841 ggaatgacga cacgccacca atgaaagcct ggtccggccg cttgtcttgc cgtggtgagg
      901 gtcgcggagc tttggctcat ggcggaggca ccgttaactg tttgcttacc agtcccgggg
      961 gagggtgctg gcgaaaatca cccaaaggcg caaaatgcgc attgagctag atcaaggccc
     1021 gtgcgaatct cctgtggcct tgtgcactcc gcgcagccga taaaggccat ggcgatgaac
     1081 gaacgcacgc tggctgtgat aacgccggag gggttgggtg gcctgatcga tggcctccgc
     1141 accgacggtt tccgggtcat tggtccgaag ctgcgcgatg gcgccatcat ctatgacgat
     1201 atcgagacgc tatccgacct gccggccggc tggaccgacg aacaggatgg cggccattat
     1261 cgtgtgcgcc ggcgcgacga tgcggcgttg ttcggctaca atgtcgggcc gcatgcgtgg
     1321 aagcggttcc tgcatccgcc gttgctgcgc ctgtggcgcg ccgagcgaag tgcagacggc
     1381 atggcaatcg ccgatacgtc ggatacggcg gagcgttatg ccttcctcgc ggtgcgatca
     1441 tgcgaactgc atgcgatcgg cattcaggat cgggtgttcc tgcaagggcc ctatgtcgat
     1501 cctcactatc gtgcgcggcg agagggcgct ttcatcattg cggtgaactg cggtgatgca
     1561 ggcggaacgt gtttttgcac ctcgatgaag accgggccta aggcacagga cgggttcgat
     1621 ctgtcagtta ccgagcttct ggcagatgag catgagttcc tggttgaggt cggcagcaag
     1681 cgtggcgccg cgttgctcgc gactgtgccg aaccggccgc cggacgcccg gcacaccacc
     1741 atggcagccg atgccactgc gcgggccgag ggcaaccagg gacgccacat gcgctcggac
     1801 gacgtgcgcg agttgttgca ggcgaacctt gagcatccgc gttgggacga tgtcgcgcag
     1861 cgctgcctga cctgcggcaa ctgcacgatg gtctgcccga cctgcttctg cacctcggtg
     1921 ttcgatgaga acgacctcgc caccggtgcc gcggaacgca agcgtcgctg ggacagctgc
     1981 ttcacgatgg acttctcgta tatccatggc ggatcggtgc gaagttcagc gcggtcgcgc
     2041 taccggcagt ggatgacgca caagctggcg aactgggtgg accagttcgg tacatccggc
     2101 tgcgttggat gtggccgctg catcacctgg tgtcctgtcg gcatcgacat caccgaagag
     2161 gtccgcgcca tccgcggcga aggaggcagc gatggaaggg cttgagcgta tcgtcgcaga
     2221 gcacaagctg ttcgaggggc ttgggcctga gttcatctca ctggcggccg gctgcgcgaa
     2281 gaacgtgcgt ttcgacaccg accagtatct gttccacgcg gatgatccgg cagactggat
     2341 ttatctggtc cgtttcggac gggtggcatt ggaaatggtg acgccgggac gtggcgccgt
     2401 gcagtacgag acgctggggc cgggcgaggt cgttggcctc acctggctgc tgccccctta
     2461 ccgctggggc tacgacgcgc gcgcgatcga actggtgcgg gctatcgcgc tcgacgcgaa
     2521 gtgcctgcgt gacaaatgcg aagtcgatca taatctcggc tatgaggtcc tgaagcgttt
     2581 cctgccggta ctggtgcaac ggcttcagtc agtccgtttg cagatgttgg acgtctatgg
     2641 atcggctcgc tgaacgcgcg ccggtgacgg acgcgatggt gccgcgcatt gcgcgcgtcg
     2701 cgcgccgcgc gcgcgatctg ccggatacct ggacgctcga tctggtgtcg ccggatggtg
     2761 ggccgctgat gcactacgcg cctggccagt tcaccatgat gtatgtgttc ggcgttggcg
     2821 aaattcccgt cagcatcagc ggcgacgctt cggatgcgac acgcctggtg cagacggtcc
     2881 gcgcagttgg caaggtgagc gaggccgtca ccaggctgtc ggccggcgac acgctgggca
     2941 tccgagggcc atatggcacg tcatggccag tcgaggaggg ctatggccgc gatgttgtgg
     3001 tggtggcggg cggtcttggc ctggccccgc ttcggccggc gctgtatcag cttcgccgcg
     3061 aacggaagcg gttcggcaag ctcgtgctgc tgtacggcac gcgccggccg gaggatatcc
     3121 tgtatcgccg tcaactggag acctggcggc ggcagctcga tatggaaatc gaggtcacgg
     3181 tggaccacgc caataccgag tggcgcggca atgtcggcgt ggtgaccaag ctgatcccgc
     3241 gtctgggcat cgatacggcc aatgcgatgg cgctggtgtg tggaccggag atcatgatgc
     3301 ggttcgccgc gagtgcgctg catgacgtcg gcatgccgga ggaggcgatt catctgtcga
     3361 tggagcgcaa catgaaatgc gcggtgggcc tgtgtgggca ctgccagttc gccggtagtt
     3421 tcatttgcaa ggacggcgcg gtgatgcgct atgaccggat tcggcgcttg ctgtcgctga
     3481 aggagattta gatgtcatcg cgagaggcgc agcgacgaag caatctcgac ttaattgtgc
     3541 gcgcgcagtc gggccgagtt tgcttcgtcg cggcgctcct cgcaatgaca cttcgcgggg
     3601 gacacctttg atgaacgccg gacgcagacc caagcttgcc gtctggaaat tcgcctcgtg
     3661 cgacgggtgt cagctttcgc tgctggactg tgaggacgaa ttgctggctg tcgccggcgc
     3721 agtcgaaatt gcgttcttcc cggaagccac gcgagcggtg gtgcgcggac cttacgatgt
     3781 gtcgctggtg gaaggctccg tcacgacgcc gcatgatgcc gagcgcttgc atacagtgcg
     3841 gcggcagtcg cggacgctga tcacgattgg cgcctgtgcg accgcgggcg gcatccaggc
     3901 actgcgcaat ttcaaggatg tccgtgagtt cacgcaggtg gtctatgctc ggccggattt
     3961 catcgacaca ttgaccacgt caacgccgat caaagcgcac gtgaatgtgg actttgagct
     4021 gcatggttgt ccgatcaaca agcatcagct ggttgaagtg ctggcggcct ttctgaatgg
     4081 ccggcagccg aacgcgccgg cgcacagtgt ctgcgtggaa tgcaaactgc gtggcacggc
     4141 gtgtgtgctg gtgcgcggca ttccctgtct ggggccggtg acgcatgccg ggtgtggcgc
     4201 gatctgcccg gcgtacaatc gcggctgcta tggctgcttc ggaccgatgg agacgccgaa
     4261 tgcgccggcg ctgagccgca agctggaagc ctgcggcatg gggcaacgtg atctgcagcg
     4321 tgtctacagc actttcaacg ccaacgagct gccgttccgc gaggaaagcg cgcgccatgg
     4381 ctcgtaagac gatcaacgtt gattaccttg cccgcgtgga aggcgagggc ggggtgaaga
     4441 tcacgttcga cgggaacaca gtgcgcgatg tgcagttacg catcttcgag ccgccgcgct
     4501 tcttcgaggc attcctgcgt ggtcgcgcat acaccgaggc gcccgacatc acggcgcgca
     4561 tctgcggcat ctgccccgtt gcctaccaga tgagcgctgt gcacgcgatg gaggacgcgc
     4621 ttggggttac tgtgacgccc gaggtgcgcg agctgcgacg tctgctttat tgcggcgagt
     4681 ggatcgagag ccacgtactg catatctaca tgctgcatgc gccggacttt ttggggtatc
     4741 cgggcgcggt cgagatggcg aaggaccatg gcgatctggt gcgccagggg ctggagctga
     4801 agaagattgg caatgagatc ctggaggtgc tcggcggacg ggcgatccat ccagtgaatg
     4861 tgcgtgtcgg ggggttctat cgcgcgccga cgcgtcggga attcgcgccg ctggctgaac
     4921 ggttgaagca cgcgcgcgat atcgcacgcg caaccgtccg ctgggtggcg ggtttcgatt
     4981 tcccggattg cgagcgcgat tatgaatacg tgtcgttggg agcggccgga gagtatccgt
     5041 tcaacgccgg gcatgttgtg tccaataagg ggctggatat cacgccgcac cagtatgacg
     5101 cgtatttcga agagtcacat gtgaagcgct cgaatgcgct gcactcgcat ttgcagggac
     5161 ggccgtatct caccggtccg aatgcccgct acagcctgaa cgcggcgcag ctttcgccgt
     5221 tggcacgcga ggccgcgcag gaggccgctt tgggcgagac ctgcacaaac ccgtttcgca
     5281 gcatcgtggt gcgtgcggtt gaagtgctgt atgcctgcga tgaggcgctg cggatcatcg
     5341 atgcctatcg cgagccggaa cagccacacc tggatctgac tccgcgtgcg ggcgtgggat
     5401 atgcatgtac cgaggcgccg cgaggcatgc tgtaccaccg ctacaggatc gatgctgatg
     5461 gcagcatcct ggatgcgaag atcgtgccgc cgacatcaca gaaccagccc agcatcgagg
     5521 atgacctcaa gacgttcgcc ggcgattggc tggacctgcc ggaggaagcg ctgcgcgtgc
     5581 gctgtgagca ggccgtgcgc aatcacgatc cctgcatttc ctgcgcgacg catttcctga
     5641 agctggaagt ggtgcgtatg tgactgggcg ggttgtgctg gccattggca acccggatcg
     5701 tggcgacgat gcggtcgggc gcttcgttgg ttcgctgctg caatcgagcg tcatgccaga
     5761 cattcctgtc accctgctca acggcgaggg cgctgggatc gtcgccgcgc tggaaggcgt
     5821 ggatgaggca tggctggttg atgccgcc
//
LOCUS       DATGEM010000242         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_768789, whole genome shotgun sequence.
ACCESSION   DATGEM010000242 DATGEM010000000
VERSION     DATGEM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768789"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VKQ27_07670"
     CDS             complement(<1..228)
                     /locus_tag="VKQ27_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLY88847.1"
                     /translation="MTENPCQTIAIRGGLVLDASGFLGPADILVQHGNIAAIGAPGMP
                     VPDDAQIIDASRRLLHPGLVNAHTHGHGNLSR"
     gene            complement(221..802)
                     /locus_tag="VKQ27_07675"
     CDS             complement(221..802)
                     /locus_tag="VKQ27_07675"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HLY88848.1"
                     /translation="MIVEAGGRFSARLAAGERLRIVDTHGQQAVDFLCYSAELPVDRY
                     NAPNTMKINGNIYLGKGSKLYSDHARVLMRIIDDTCGRHDTIGGCCSTWSNFTRYGVS
                     DAPGCHQNFIAELANWSLSPSDVVANVNFFMNVPVRQDGAMAIEPGLSKPGDYVELIA
                     EIPVIAILSNCPQRFNPAAGFGPTPIEVLIRRD"
     gene            complement(807..2063)
                     /locus_tag="VKQ27_07680"
     CDS             complement(807..2063)
                     /locus_tag="VKQ27_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HLY88849.1"
                     /translation="MDRQFDLLLRGGRVIDPATGHDGIADVAVTDGRITAIGPSLPPA
                     AAQVIDATGKIVIAGMIDTHAHIYQHVTGKFGLNADMVGIQSGVTTVVDQGGPSCMTI
                     PGFRHFVVEPSKTRVLCFISAYLVGGLEGHLYPALYGPDQVDVKATIRAGRANADIVR
                     GVKAHAEIGGASRWGLEVIKLGKRISSELGVPLYIHLGQIWPVKDGATIDPDEVVREL
                     MPLMEPGDVLAHPFTRHPGGFVSTATGEVHPVVWAALDRGVTVDVGHGSHFSFEMARR
                     TLAAGIRPHTLGADMHGYNVRVPGEGDTAKSDNPFVGVAPFNLTHAMTELMTLGIPLP
                     EIVATVTSNAAKMLKMEDSIGSLQPGREADISVLDLLHGRFTLSDNSGETVIAPDLLT
                     PAFAIRAGKVMQSDSALIPPPALLAA"
     gene            2144..>3520
                     /locus_tag="VKQ27_07685"
     CDS             2144..>3520
                     /locus_tag="VKQ27_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY88850.1"
                     /translation="MEEGVGQSVPRLEDERYLRGKGEFIADIRLAGMRDLAFVRSPVA
                     HARIRGVRKPEGAVFAAADLVGVQPIVAVSGLPGFKPSVQPVLASDKVRHVGEPIAVC
                     VADSRAEAEDLAAAVEVDFEELPAVVDMRAALNSPTKLHEHWDNNIFLETFIEVAPEI
                     FARPAPIVVRRKLRTARQCMSPLEGRGVVSTWDRRLEQLLVYTSTQLPHIVRTGLAGC
                     LGLDQARIRIVAPDVGGGFGYKGILLQEEVCAAWLARHLGYPVRWIEDRREQLTGNAN
                     CREHDYDLTVYAEADGTLIGIDADAVVDAGAYSAYPFSACLEAAQVASILPGPYTMQA
                     YRCRTFSAATNKPPILPYRGVARAGVCFALEVALDAVARQAGLEPHEVRLRNLVPADR
                     MPFENITKKYFDSGDYPQCLRRVIEAIDLPAVRRRQAEATGAVRIGFGCAIFCEQAAH
                     GTTVYAGWG"
BASE COUNT          578 a          933 c         1325 g          684 t
ORIGIN      
        1 gcgcgagaga ttgccgtggc cgtgcgtgtg cgcgttcacc agaccggggt gcagcaggcg
       61 acgtgatgca tcgatgatct gtgcgtcgtc tggcacgggc atgccggggg cgccgatcgc
      121 ggcgatgttg ccgtgctgca cgaggatgtc ggcgggaccg aggaagccgc ttgcatcgag
      181 caccaatccg ccacggattg cgatcgtttg gcaggggttt tcagtcacgg cggatcagaa
      241 cctcgatggg tgtggggcca aagccggcgg cggggttgaa gcgttgcggg cagttggaaa
      301 gtatggcgat caccgggatt tcagcgatca gttccacgta gtcgcctggc ttggagaggc
      361 cgggttcgat ggccatcgcg ccgtcctggc gaaccgggac gttcatgaag aagttcacgt
      421 tcgcaacgac gtcggagggc gagagcgacc agttggcgag ttcggcgatg aagttctggt
      481 ggcagccagg cgcgtcgctg acgccatagc gcgtgaagtt gctccaggtg ctgcaacagc
      541 caccgatggt atcgtggcgg ccgcaggtgt catcgataat gcgcatcagc acccgggcgt
      601 ggtcggagta gagttttgag cctttgccca ggtagatgtt gccgttgatc ttcatcgtgt
      661 tgggtgcgtt gtagcgatcg accggcagtt cggccgaata gcacaggaaa tcgaccgcct
      721 gctggccgtg tgtatctaca atgcgcagcc gctcgccagc ggcgagccgg gcggagaaac
      781 gcccgccagc ttcgacgatc atgggatcag gccgcgagca gggcgggggg agggatcagc
      841 gcgctatcgg actgcatgac cttgccggcg cggatggcga aggctggtgt cagcaggtcg
      901 ggggcgatga ccgtctctcc gctgttgtcg gagagtgtga agcgaccgtg caggaggtcg
      961 agcaccgaaa tgtcggcctc gcggccaggc tggagcgagc cgatgctgtc ttccatcttc
     1021 agcatcttcg ccgcattgct ggtgacggtg gcgacgattt cggggagcgg gatgccgagc
     1081 gtcatcagtt cggtcatcgc gtgggtcaga ttgaagggtg cgacgccgac gaagggattg
     1141 tctgacttcg cggtatcgcc ttcgccgggg acgcgcacgt tgtagccgtg catgtcggcg
     1201 ccgagggtgt ggggacggat gccggcggcg agcgtgcggc gggccatttc gaagctgaaa
     1261 tgcgagccgt ggccgacgtc gacggtgacg cctcggtcga gcgccgccca gacgacggga
     1321 tggacttcgc cggtggcggt ggagacgaag ccgccggggt ggcgggtgaa gggatgtgcc
     1381 aatacgtcgc cgggttccat caatggcatg agttcgcgca cgacttcgtc ggggtcgatg
     1441 gtagcgccgt ctttcaccgg ccagatctga cccaggtgga tatacagggg aacgccgagc
     1501 tcgctggaga tgcgcttgcc gagcttgatg acttccaggc cccagcgcga ggcgccgcca
     1561 atctcggcat gtgccttcac gccgcggact atgtcggcgt tggcacggcc ggcgcggatg
     1621 gtggccttca cgtccacctg gtcgggtcca tacaaggcgg gatacagatg gccttcgagg
     1681 ccaccgacca gataggccga aatgaagcac aggacgcggg tcttcgacgg ttccacgacg
     1741 aagtggcgga agccggggat ggtcatgcag ctcgggccac cctggtcgac cacggtggtg
     1801 acgccggact ggatgccgac catgtcggcg ttcaggccga acttgccggt gacgtgttga
     1861 tagatgtgcg catgggtatc gatcatgccg gcgatgacga tcttgccggt tgcgtcgatg
     1921 acctgcgctg ctgccggtgg gagcgagggt ccgatggcgg tgatgcggcc gtcggtgacg
     1981 gcgacgtcgg caatgccgtc gtgacctgta gcggggtcga ttacgcgtcc gccacgcagc
     2041 agcagatcga attgcctgtc catcgaaatg acctcgccaa ttgtcggagt gcccggatcg
     2101 tcagtatgct gacggcttgg cgtttggtgc aagcgggggg ttcatggagg agggcgtcgg
     2161 ccaatcggtg cctcggctgg aagacgagcg ctacctgcgc ggcaaggggg agttcatcgc
     2221 ggacatccgc ctggctggta tgcgcgatct ggcatttgtg cgcagtcccg tggcgcatgc
     2281 gcggatccgg ggggtgcgca aacccgaggg ggcggtgttc gcggccgcgg atctggtggg
     2341 cgtgcagccg atcgttgcgg tatcaggatt gccggggttc aagccgtcgg tgcagccggt
     2401 gctggcatca gacaaggttc ggcatgttgg tgagccgatc gcggtgtgcg tggcggatag
     2461 ccgcgcggag gcagaggatc tggcggccgc cgtcgaggtt gatttcgagg aactgcccgc
     2521 ggttgttgac atgcgcgcag cgctgaacag cccgacgaag ctgcacgagc actgggataa
     2581 caacattttt ctggagactt tcatcgaggt tgcgccggag atatttgcgc gaccggcgcc
     2641 tattgttgtg cggcggaagc tgcgcacggc gcggcagtgc atgtcgccgc tggaggggcg
     2701 tggtgtcgtt tcgacatggg atcggcggct tgagcaactg ctggtatata cgtcgacgca
     2761 gttgccgcac atcgtgcgga ccgggctggc ggggtgcttg gggctggatc aggctcgtat
     2821 ccggatcgtg gctccggatg ttggcggtgg gtttggctac aagggcatcc tgctgcagga
     2881 ggaggtctgt gccgcgtggc tggcgcggca tctgggctat ccggtgcggt ggatcgagga
     2941 tcgccgcgag cagcttaccg ggaatgcgaa ctgccgcgag catgactatg acctgactgt
     3001 ctatgctgag gccgatggga cgctgatcgg catcgatgcg gatgcagttg tggatgcggg
     3061 tgcatattca gcctatccgt tctcggcgtg tctggaggcg gcgcaggtcg cctctatttt
     3121 gcctggaccg tatacgatgc aggcttatcg ctgccgcacg ttttccgcag cgacgaacaa
     3181 gccgccgatt ttgccttatc gcggggtggc gcgggcgggc gtgtgcttcg cactggaagt
     3241 ggcattggac gcggtggcac ggcaagcggg actggaaccg catgaggtca ggctgcggaa
     3301 tctggtgccg gcggatcgga tgccattcga gaatattacg aagaaatact ttgatagcgg
     3361 cgattatccg cagtgtttgc gcagggtgat cgaggcgatt gatcttccgg cggtacgccg
     3421 gcgtcaggcg gaagcgacgg gggcggttcg gatcggtttt ggctgcgcga tcttctgcga
     3481 gcaggccgcg catggcacga cggtctatgc cgggtggggc
//
LOCUS       DATGEM010000243        13333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_820104, whole genome shotgun sequence.
ACCESSION   DATGEM010000243 DATGEM010000000
VERSION     DATGEM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820104"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1095)
                     /locus_tag="VKQ27_07690"
     CDS             complement(154..1095)
                     /locus_tag="VKQ27_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HLY88851.1"
                     /translation="MTVDLLCMGEPMLEFNQLPAQPDGTQHYLEGHGGDTSNAAIAAS
                     RQGARVGYITALGQDMPGDSFVALWARENVDASTVIRSDRWQTGVYFVTHDAQGHHFL
                     HYRTNSAAAMYTQADLPTAAIAAARLFYVSGISQGISTSAADAVFAAIDIAKRNTGLV
                     AYDTNYRPRLWPPARAASIMHAAIAQCDYALPGIDDVHTLTGLTEPEAMLDFYLRLGP
                     KVVVLKMGEHGAWIATPDQRQRIPPHRVNVVDATGAGDTFCGSFLARILAGDAPEAAA
                     RYAGVAAALKCTGYGAVAPIPRAAEVRAAMQRGNSNV"
     gene            complement(1092..1715)
                     /locus_tag="VKQ27_07695"
     CDS             complement(1092..1715)
                     /locus_tag="VKQ27_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HLY88852.1"
                     /translation="MPESLLDTLRAARVVPVVRTQETRHAATVVQWLRDAGLRIFEIT
                     MTIPDAPSLIRELARDSRLLVGAGTVPDFATAETCLNAGARFIVAPWIDTSLAAPCRA
                     AGATLMLGALTPTEVRTALSAGADVVKVFPASSVGGPAHIRALRSVLPDVAFCPTGGV
                     EPDNVAVYLAAGAAFVGMGGALVDEKRIAAGDRGAIEATARQVLSAS"
     gene            complement(1875..2192)
                     /locus_tag="VKQ27_07700"
     CDS             complement(1875..2192)
                     /locus_tag="VKQ27_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88853.1"
                     /translation="MSGTGETARIIPFPVRRNAAALDEGQERLRRALEGLDSAIAGQR
                     VAVAAWRKALGELGTVVSGLGESLHSYRGNLDKLGERVAGLNSQARQLEQTADAALAS
                     RPK"
     gene            complement(2259..2726)
                     /locus_tag="VKQ27_07705"
     CDS             complement(2259..2726)
                     /locus_tag="VKQ27_07705"
                     /inference="COORDINATES: protein motif:HMM:NF023338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
                     /protein_id="HLY88854.1"
                     /translation="MPLIAILLSALGLIPLIGCALGALGPDPVTASHMLQALIGYSAL
                     ILAFAGGIHWGFELQTPQANRLVGRLRLAIPAIALLAAWAALMLPLVVAPASALIVLI
                     AAYLGAILVEQRAVAYGALPQPYLWLRWGFTIPAIAMLITAFTLRLLGQTIEF"
     gene            2791..4089
                     /locus_tag="VKQ27_07710"
     CDS             2791..4089
                     /locus_tag="VKQ27_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLY88855.1"
                     /translation="MGLGLEIVIVVLLILVNGLFALSELALVSARRARLAMLEHKGVA
                     GAARARELAQDPQRFLPTVQVGITLVSMLTGVFGGARIAGDLKTLLASFPGLAPAAET
                     LSLVLVVVVTTYLTMVLGELVPKHLALRRPEMAAVKVAPAIAFVARVSGPIVWLLNIS
                     SAGVLRLFGLHRVARQTVTEEELKALLVEGTQAGVLETEERDIIERVLRLADKPVRAI
                     MTPRTEIAWIDRTDSPKDIAAELKAAPHSRFVVCDGSVDNVVGVVRAKDLLDRILDGG
                     DLALGAALRQPMVVPDTVTALDSLERLRLDPLGMALVMDEYGSFEGVVTAADVLEAIV
                     GDPTDAAPQLADSSGEGEAALVMDGMMPVDELKARLSLPDLPAEGSYHTLAGLVLALL
                     RRVPRVGDRIVFSGWRFEVLEMDGRRVDKVRASREPAAET"
     gene            4164..5636
                     /locus_tag="VKQ27_07715"
     CDS             4164..5636
                     /locus_tag="VKQ27_07715"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HLY88856.1"
                     /translation="MSELLHRVEAASTSQLAPIGLPLCIDLDGTLLRIDSLFEATFSA
                     VLADWRVLFQLPRWLAEGRAHLKQELAARWEFVAAQLPYDQDVVQLIELERARGRRIV
                     LCTAADERIANAVAAHLGLFDDVIASDGSSNLRGERKAAELCRRFGEHGFVYAGNDST
                     DYAVWRHAAAAVVVNAPSSVARTAAELCRVAATIDDRAPRMRAALRALRPYQWVKNVF
                     TLVPLLAAGDFANGAGWRHSLAIMAAFCATASAIYLINDLSDLAADRAHPRKRLRPFA
                     SGALSLASGLAMVPVLFVLGAWLGLASGAVEALAVYAALSLGYTIRLKEQPLIDVFIL
                     AALYTIRLFGGGEASGHPVSMWLLGFSSFLFLSLALVKRVSELYRLQSAALSKTERRG
                     YVVQDLPMLQMFGCASSFASAVVLSLYVQSDTALHAYARPSMLWGVIPLLLFWQCRLW
                     LSASRGYMNDDPIVYAARDWVSWLVFAGVVGVAVAAWLPV"
     gene            5654..6034
                     /locus_tag="VKQ27_07720"
     CDS             5654..6034
                     /locus_tag="VKQ27_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLY88857.1"
                     /translation="MITWQIFALVLISVGGSAFGQIALKFGMASASVQAALTTGGVVP
                     VSTAIVINPFVLSGLFLYGFSAVVWLFVLARLDVSIAYSFVSLGFIVIMSLGCLLFGE
                     PFTLRKLLGTIVVMLGVYLVAAVR"
     gene            6031..7644
                     /locus_tag="VKQ27_07725"
     CDS             6031..7644
                     /locus_tag="VKQ27_07725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY88858.1"
                     /translation="MIARLELAFAAVVFLAGAMLLFVTAPHHGEFWWSDAPRHALNGA
                     FVKDMVAAMPAHPAAWAMQYYVKYPALTILFYPPLFYVILAPFYAVFGVSHATAVAVE
                     LLHYFALSFGLYMLVRRWVGPLTAIAVGLSAMAAPGIALWGRQVMLEVPSVAFAVWSA
                     VLLEDHLASGRLSRLYLAMFLLLCATYTKITTIFLFPVFALVLIVAQGWQELGKRQIW
                     FVIVFTVLGLIPVAFLTLKFGQANVQSVTGIPDARVARDSIAGWTWYVRQMPWQLGWP
                     LLALAVLAIPLGIAGRLSPRLSLADLTLICGWIVLGYLFLSLIDLKEARHALVFLPMV
                     LVAAGLSLDTLLPARATGPTLMLLVVGTGIYTWLQAPTPFVDGYREAAEWIAREAPKE
                     AIVVFSGKRDGSFIFDMRAIKVRGDISIIRSDKLLLDIAVRRTIGVQQKDLSEQQIGD
                     LLDRDGVSYVVAQDDFWTDLPEMARFQAVLRSPHFQVEEHIPVVSNVPTEDRNLTIYR
                     NLGDVSSGPHMVDLHLPIIGETVKGTVGK"
     gene            7641..8849
                     /locus_tag="VKQ27_07730"
     CDS             7641..8849
                     /locus_tag="VKQ27_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HLY88859.1"
                     /translation="MTVRQCAVLVGGRGSRLGALTAETPKPLLPCGDRPFLAWVMREF
                     IRFGVEEFVLLAGYRAEHVTAAVPKLAALLPRPVRILVSEETEPAGTGGALFHARALL
                     DQRFLLCNGDSLFDTNMAPLVTADARALMLLRRVDDASRYGVVSLAGGRVSGFDAVST
                     TSSPGIINAGVYLLDRSLINECTPCCSLEADILPRIAASGLLHGMVADGYFRDIGVPE
                     DFARAQSEVPGVLRRSALFLDRDGVVNVDHGYIGSRERFEWTAGALEAISAATGAGWH
                     VFVVTNQSGVARGYYDEAAVHTLHAWMVSEVRQFGGTIDDIRYCPYHEQATVAAYRRA
                     SDWRKPAPGMLLDLMRAWELEPERCILIGDQETDMAAARAAGMKGRRFAGGNLADFVR
                     PTLERFACRC"
     gene            8837..10861
                     /locus_tag="VKQ27_07735"
     CDS             8837..10861
                     /locus_tag="VKQ27_07735"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY88860.1"
                     /translation="MSLLSGFRVVQLGRGLAAAVCGRLLADIGADVTCVDADTSTPLA
                     AYLNRGKTIGTRDALASANLIVRESDDGVVDTTAAQVFISPFGRTGAKANAPASDLTL
                     MYASGIARLLTGQVDDLNEAPIRPIGEQSAFIGGLAAACAGMHAAQQHMSGAIIDVSI
                     EEALATMAMTELAGAALHGRSRSRKRITDGNGATVCILPARDGFTAISPREDRQWAAW
                     LQAMGSPAWGEDPRFRTKADRVANWNALHALMSEWSRRYDKQRIADTAQAARVPSFPL
                     RELAEQLDSPQLEHRNYWQAWDGAKVPGSPFGLTMTPAQRTAAAGPLPLSGVRVLDFS
                     WVIAGPTTTRYLAAMGADVIKVEAPGRGDPGRASELHTVLGQAKRAIVLDLKQPEAVD
                     IARRLAERCDVLVENFATGVMDRLGLGASALRECNPGLIYVSASGLGRSGPESHAVAY
                     GTLLQCYAGFAGLNRHPEIAPRVGFAWLDPMCGLMLAFITAAAIWRRRATGETARVDF
                     SMIEAMLWTLAGPLLATQRGTPPAPQGNRSDGHLLHGAWRCAGDDAWISIAVANNQQQ
                     QCLLDVIGDASLEAWLAQRTAADAEATLLRAGVPAAALASSTDLVASEHLRARGFWDG
                     GLPGLPWRASFGRRWGDAPKLGADTDAVLAEVLGCQEPDIRALRARGALG"
     gene            complement(10868..11425)
                     /locus_tag="VKQ27_07740"
     CDS             complement(10868..11425)
                     /locus_tag="VKQ27_07740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY88861.1"
                     /translation="MEPVARVTVTVTFLRMDRPPAVPSPGLPPDFQVVRATAPTVGFY
                     RYLYNTVGADYVWWLRRTVADDEIAALLSDPAVSIHVLYSNGEPAGFYELDGRGWPDI
                     NLSYFGLMPHAIGAGVGYPFLRNAVDTAWRENPRGMTVNTCTADHPRALPTYLRAGFR
                     TLRQVREVWNVPLRLGMRIPERLLT"
     gene            complement(11532..12452)
                     /locus_tag="VKQ27_07745"
     CDS             complement(11532..12452)
                     /locus_tag="VKQ27_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HLY88862.1"
                     /translation="MTLVPNIPSAPSLAQGAGGDLTATWTVPPVDSSHGAATAFNLQF
                     SLAGANAWTVLSNVTRPHLLVGLTFGAAYDVQVQSLNAAGASAWSATSSLSTASGASV
                     PNAPSISFVAPPPDGTNSVLTVAWMPPAADGDHPAAAGYNLRYSPSGAGTWTTISGVT
                     SPYSISGLSGAAAIDVEVQATNASGNAGAWSATTTASTWGATVAAGNLAMATTQVHNT
                     GVAPAGGANLTATPAPTAVTGAAFAWSASSSTVPTTGLIVAGSDGQPNGWGQYFDAPA
                     TAGTYYLWMLAQGASNVTIGALVSSAIVVT"
     gene            complement(12687..13333)
                     /locus_tag="VKQ27_07750"
                     /pseudo
     CDS             complement(12687..13333)
                     /locus_tag="VKQ27_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620998.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         2084 a         3938 c         4588 g         2723 t
ORIGIN      
        1 cgcgcattgc cctccatcag ccggcgcagt gcttcatcac cggtgatcga agtttcgggg
       61 ggagggcccg cggcggcgag ggcgaggacg gcgcctgtga ctcctatcag gcgacggcgc
      121 gacagccccg gcggtggcgc cgggacacaa ttttcacaca ttgctgtttc ccctttgcat
      181 ggccgccctg acctcggcgg ctcgtggtat cggcgcgacc gcgccatagc cggtgcattt
      241 cagcgcggcc gcgacgccag cgtagcgcgc ggcggcctcc ggtgcatcgc ctgccaggat
      301 gcgagccaga aaactgccgc agaacgtgtc gccagcgcca gttgcgtcga cgacgttcac
      361 gcgatgaggt gggattcttt ggcgttggtc gggcgtcgct atccaggcgc catgctcgcc
      421 catcttcagg accacgacct tgggtcctag acgcagatag aaatcgagca tggcctcggg
      481 ctcagtcaag ccggtcagcg tatgcacatc gtcgatcccg ggcagggcgt agtcgcattg
      541 tgcgatcgcc gcgtgcatga tggaagcggc acgcgccggc ggccacaggc gcggtcggta
      601 attggtgtca taggcgacca ggccggtatt gcgctttgcg atgtcgatgg ccgcgaagac
      661 ggcgtcggcc gcggacgtgg aaatcccctg gctgatgccc gaaacataga acaggcgcgc
      721 cgcggctatc gccgccgtgg gcaggtcggc ttgtgtatac atcgcggccg cggagttggt
      781 gcggtaatgc aggaaatgat gcccctgcgc gtcgtgcgtc acgaagtaaa cgccggtctg
      841 ccaacggtcc gaacggatca cggttgaagc gtcgacgttt tcccgtgccc acagcgccac
      901 gaagctgtcg ccgggcatat cctggccaag cgccgtgatg taccccacgc gtgcgccttg
      961 cctggatgct gcgattgccg cgttggacgt atcgccaccg tggccttcaa gatagtgctg
     1021 ggttccatcg ggctgtgcgg ggagctggtt gaattccagc atcggctcgc ccatgcacaa
     1081 caaatcgacc gtcatgacgc gctgagcacc tgtcgggcgg tggcttcgat cgcgccccga
     1141 tcgcccgcgg cgatccgttt ttcgtcgacc agagcgccgc ccatgccgac gaatgccgcg
     1201 ccggctgcga gatatacggc gacgttgtcc ggctcgacgc ctccggtcgg gcagaatgcg
     1261 acatccggca gcacgctgcg cagcgcgcgg atgtgtgctg ggccgccgac ggacgatgcc
     1321 gggaacacct tcacaacgtc ggctccggct gaaagcgcgg tgcgcacttc cgttggggtc
     1381 agcgcaccga gcatcaacgt ggcgcccgcg gcacggcagg gcgcggccag cgacgtgtcg
     1441 atccacggcg caacgatgaa ccgcgcgccg gcgttcaggc atgtctctgc cgtggcgaag
     1501 tccggcaccg tgccggctcc caccagcagg cgcgagtcgc gcgcgagctc gcggatcaaa
     1561 gacggcgcgt ctggtatggt catggtgatt tcgaagatgc gcaggccggc gtcgcgcagc
     1621 cactgcacga ccgtggcggc gtgccgcgtt tcctgagtac gcacgacagg cacgacgcgg
     1681 gctgcacgga gcgtgtccag caggctttct ggcatcgatc cacctccagc tgcgcgtact
     1741 gacaatgacc cgatgactgg tcttggacaa ctcgggcagg ccacgcggag caggtattgt
     1801 ggaggctaca tgcctatcgg gtaccgggtg ctacaggctt tgagggtagc gacgctgcac
     1861 ggaccggcaa actgttactt agggcggctc gccaaggcgg catccgctgt ctgctccagt
     1921 tgccgcgcct ggctgttcag gccggctacg cgctctccca gtttgtccag attgccgcga
     1981 tagctgtgca ggctctcgcc cagccccgac accacggtgc ccaattcgcc gagcgccttg
     2041 cgccacgccg ccacggccac gcgctgtccg gcgatcgcgc tatcgaggcc ttccaaggcg
     2101 cgccgcaacc gctcctgccc ttcatccagc gctgctgcat tccgccggac cgggaagggg
     2161 ataattcttg cagtttcgcc tgtcccggac atcgtcattt gccctctacc gccaatggtt
     2221 aataatgcct aaatcgaggg taaaaatcaa gaaaactgtc agaattctat tgtctgtccc
     2281 agcagccgaa gcgtgaatgc ggttatcaac atggcaatgg cagggattgt gaagccccag
     2341 cgtagccaca gatagggttg cggcaaggcg ccgtaggcca ccgcgcgttg ctccaccagg
     2401 atcgctccaa ggtaggcggc aatcaggacg atgagcgcgc tcgcgggcgc gacgacgagg
     2461 ggcagcatca gggccgccca ggccgcaagc aatgcgatgg ctggtatggc cagccgcaat
     2521 cgtcccacga gacggttcgc ctgtggcgtt tggagttcga acccccagtg aatgccgccg
     2581 gcaaaggcga ggatcagcgc tgagtacccg atcagtgcct ggagcatatg cgaagcggtg
     2641 accgggtccg gtccgagggc acccagcgca cagccgataa gcggtatcag tccgagcgca
     2701 ctcagcagga tggctatcag cggcatggca ggcatcagag cgccgaaccg tgctagctga
     2761 caaccgccct gccgcggtta tgctggtgcc atgggcctcg gactcgaaat cgttatcgtc
     2821 gttctgctga tcctggtgaa cgggttgttc gcgctcagcg agctggcgct ggtttcggcg
     2881 cggcgcgcac ggctggcgat gctggaacac aagggagttg ccggcgctgc gcgcgcacgt
     2941 gagctggcac aggacccgca gcggttcctg ccgacagtgc aggttgggat cacgctggtc
     3001 agcatgctga ccggtgtttt tggtggcgcg cgcattgccg gcgatctgaa gaccctgctc
     3061 gccagctttc ccgggcttgc gcccgcggcc gagacgctgt cgctggtgct tgtcgttgtc
     3121 gtgacgacct atttgaccat ggtgctgggc gagctggtac ccaagcacct ggcattgcgg
     3181 cgacccgaga tggcggccgt caaggtcgcg cccgccattg ctttcgtcgc acgggtcagc
     3241 gggccgattg tttggttgct caacatttcg tcggccggcg tgctgcggct gttcggcctg
     3301 caccgtgtag cgcgtcagac ggtcaccgag gaggagctaa aggctctgct ggtcgagggc
     3361 acccaggccg gtgtgctgga aaccgaggag cgcgacatta tcgagcgcgt gctccgtttg
     3421 gctgacaagc cggtgcgtgc gatcatgacc ccccgcaccg agatcgcctg gatcgaccgg
     3481 accgattcgc cgaaggatat cgccgccgag ctgaaagccg cgccgcactc gcggttcgtg
     3541 gtgtgcgacg gatcggtcga caatgtggtc ggcgtggtac gggcgaagga cctgctggat
     3601 cgcatcctgg atggcggcga cctggcgctt ggagctgcgc tgcgtcagcc gatggtggtg
     3661 cctgatacgg taacggctct cgattcccta gagcggctgc ggctggatcc gctggggatg
     3721 gcgctcgtga tggatgaata cggcagtttc gagggggttg tgaccgcagc cgacgtgctg
     3781 gaggcgattg ttggtgaccc gaccgatgcg gcgccccagt tggcagatag cagtggcgaa
     3841 ggcgaggccg cactggtgat ggacggcatg atgccggtgg acgaattgaa ggcacgactt
     3901 tcgctgccgg atctgccggc cgagggcagc taccacacgc tcgcgggcct ggtcctggcg
     3961 ctgttgcgcc gggtcccgcg ggtcggcgac cgcatagtat tctccggctg gcggtttgaa
     4021 gtgctggaga tggatggccg gcgcgtcgac aaggtgcgtg cgagccgtga gccggcagcg
     4081 gagacgtagt cggcatcccc gcaaagcttg ctccggccgg gtagaacccg gcataggaag
     4141 ccgtccactc gcccgaggcc gatttgtcgg aattgctgca tcgcgtcgag gccgcatcca
     4201 ccagtcaact ggctcccata ggcttgccgc tttgcatcga tctggacggc acgctgctcc
     4261 gcatcgattc gctctttgag gccacgtttt ccgccgttct ggcggactgg cgcgtcctgt
     4321 tccaattacc gcggtggctt gcggagggcc gcgctcacct gaagcaggaa ctcgcggccc
     4381 gatgggaatt tgtcgcggca caactgccgt acgatcagga tgtggtgcaa ctcatcgaac
     4441 ttgagcgcgc ccgaggccgt cgtatcgtgc tttgcacggc cgccgatgag cggatcgcga
     4501 atgcggtcgc cgcacatctg ggcttgttcg acgacgtgat tgcgtcggat ggcagcagca
     4561 atctgcgtgg tgagcgcaag gcggccgagc tgtgccgccg cttcggcgag cacggctttg
     4621 tctatgctgg caacgacagc accgattatg cggtatggcg acacgccgcg gcggcagttg
     4681 tggtgaatgc gccatcgtcc gttgcgcgca ccgcggcgga actgtgtcgc gttgccgcaa
     4741 cgatcgatga tcgtgcgccg cggatgcggg ctgcgctgcg agcgttacgg ccgtaccagt
     4801 gggtgaagaa cgtgttcact ctggtgcctc tgctcgccgc gggggacttc gccaacggcg
     4861 ccggatggcg gcattcgctc gcgatcatgg cggcattctg cgccaccgca tcggcaatct
     4921 atctgatcaa cgatctctcg gaccttgctg cggaccgcgc gcatccgcgc aagcggctgc
     4981 gcccgttcgc cagtggcgcg ctttcgctgg cgagtggcct ggcgatggtg cccgtgctgt
     5041 tcgtgctcgg tgcctggctg ggtctcgcca gcggagcggt cgaggcgctc gctgtatatg
     5101 ccgcgctgtc gcttggctac acgatccggc tgaaggaaca gccgctgatc gacgtgttca
     5161 ttctggcggc tctctacacc attcggttgt tcggcggcgg tgaggcgagc gggcatccgg
     5221 tttccatgtg gctgctgggg ttttcctcgt tcctgttcct cagtctcgcg ttggtgaagc
     5281 gcgtttcgga gctgtaccgg ctgcaatcgg cggcgctgtc gaagaccgag cggcgcggct
     5341 acgtggtgca ggacctgcca atgctgcaga tgttcggctg cgcttccagc ttcgcctctg
     5401 cggtcgtgct gtcgctatac gtacagagcg acacggccct gcatgcctat gcgcggccat
     5461 cgatgttgtg gggagtgatc ccgttgctcc tgttctggca gtgccgcctg tggctttctg
     5521 ccagccgcgg atacatgaac gatgacccga ttgtctatgc agcgcgggat tgggtctcgt
     5581 ggctggtgtt cgccggggtg gtgggcgtcg cggtcgcagc ttggttgccg gtatgaatag
     5641 gatttcgtct cccgtgatca cctggcagat ttttgcattg gtcctaatca gcgtcggcgg
     5701 ttcggcattc ggacagatag cgctgaaatt cggcatggcg tcggcttcgg tgcaggcggc
     5761 attgaccacc ggtggtgtcg tgccagtcag cactgccatt gtgatcaatc cgttcgtgct
     5821 gagtgggttg ttcctctacg ggttcagcgc ggtggtctgg ctgtttgtgc tggcgcggtt
     5881 ggacgtgtcg atcgcctatt cgttcgtgtc gctgggcttc atcgtcatca tgtcgctcgg
     5941 ctgtctgctg tttggtgagc cattcacctt gcgcaagttg cttggcacca tcgtggtcat
     6001 gcttggcgtg tatctggtgg ctgcggtgcg atgattgccc ggcttgagct tgcgtttgcc
     6061 gcggtcgtgt ttcttgcggg cgcgatgctg ctgtttgtca cggcgccgca tcacggtgag
     6121 ttctggtgga gcgatgcacc gcggcacgcg ctgaatggcg ctttcgtcaa ggacatggtt
     6181 gccgccatgc cggcgcatcc tgccgcgtgg gcaatgcagt actacgtgaa gtatcctgcg
     6241 ctgacgatcc tgttctaccc gccgttgttc tatgtgatcc tggcgccgtt ctatgcggtg
     6301 ttcggcgtgt cgcatgccac cgcggtagcg gtcgagctgc tgcactattt cgcgctgtcg
     6361 ttcgggctgt acatgctggt gcggcgctgg gtcggtccgc tgaccgccat tgcggttgga
     6421 ttgtcggcga tggccgcgcc cggcattgcg ctatgggggc gccaggtgat gctggaggtc
     6481 ccgtcggtcg cgttcgcggt gtggtcggcc gtactgctgg aggaccatct cgcgagcggg
     6541 cgtttgtcca ggctgtacct ggcgatgttt cttctgctgt gcgcgaccta caccaagatc
     6601 acgacaatct tcctgttccc ggtttttgca ttggtgctga ttgtcgcgca ggggtggcag
     6661 gaattgggca agcggcagat ctggttcgtg attgtgttca ccgtgctggg cctcattcct
     6721 gttgcatttc ttacgctcaa gttcggccag gcaaacgttc agtcagtcac cggcattccc
     6781 gatgcgaggg tagcgcgcga cagcatcgcg ggctggacct ggtatgtgcg gcagatgccg
     6841 tggcagctgg gatggccgct gctggcattg gcggtgctgg cgattcccct gggcattgcc
     6901 ggccgtctct ccccacgact gagcttggca gatctcacgc tgatctgtgg gtggatcgtg
     6961 ctgggctacc tgttcctgtc cttgatcgat ctgaaggaag cccgccacgc gctggtcttt
     7021 ctgccgatgg tgctggttgc ggcgggtctt agccttgata ccttgctgcc ggcgcgtgcg
     7081 acggggccca cgctgatgtt gctggtcgtt ggcaccggta tctacacgtg gctgcaggcg
     7141 ccgaccccat ttgtggacgg ctatcgcgag gcggctgagt ggatcgcgcg cgaggcccca
     7201 aaggaggcaa tcgtggtgtt ttccggcaag cgcgacgggt cgttcatctt cgacatgcgg
     7261 gcaatcaagg tacggggaga catatctatc atccgctccg acaagctcct gctggacatt
     7321 gcggtgcggc gcaccatcgg cgtgcagcaa aaggacctgt cggagcagca gatcggcgat
     7381 ctgctggatc gtgacggcgt cagctatgtg gtggcccagg acgacttctg gactgatctg
     7441 cctgaaatgg cgcggtttca agctgtgctt cgttcaccgc atttccaggt ggaggagcat
     7501 attcccgtcg tgtcaaatgt gccgacggag gatcggaacc tgacgatcta ccgcaacttg
     7561 ggcgatgtgt cctcggggcc gcacatggtg gatctgcatc tgccgatcat cggtgagacg
     7621 gtgaagggca ctgtgggcaa gtgacggtcc gccaatgtgc tgtgctggtt ggggggcgtg
     7681 gttcgcgtct tggcgcgttg acggccgaga cgccgaagcc gttgctgcca tgtggtgatc
     7741 ggccatttct cgcctgggtg atgcgcgaat tcatccgctt cggcgtcgag gagttcgtgc
     7801 tgctggcggg gtatcgtgct gagcatgtca cggcggcggt gccgaagctg gcagcgctgt
     7861 tgccgcgtcc ggtccggatc ctggtgtcgg aagagacgga gccggccggc acgggcgggg
     7921 cgttgtttca tgcgcgggcg ctgctcgatc agcggttctt gttgtgcaat ggcgattcgt
     7981 tgttcgacac gaatatggcg ccattggtca cggcggatgc tcgggcgctc atgctgctcc
     8041 gccgcgtaga cgatgcgtcc cgttatggcg tcgtgtcttt ggctggcggg cgcgtcagcg
     8101 gttttgatgc agtgtcgacg acatcgtcgc ccgggatcat caatgcgggt gtatatctgc
     8161 tcgatcgctc tctgataaat gagtgcacgc catgctgctc gctggaggcc gacattcttc
     8221 cccgcattgc cgcgagcgga ttgttgcatg gcatggttgc cgacggctat ttccgtgaca
     8281 tcggtgttcc agaggatttc gcccgcgccc agtcggaggt gcctggtgtg ctgcgtcgtt
     8341 cagctctgtt tctcgatcgt gacggtgtgg tgaacgtgga tcacggctat atcggcagcc
     8401 gcgagcgctt cgaatggacg gctggtgcgc ttgaggcgat aagcgctgct acgggcgctg
     8461 gatggcacgt cttcgtcgtg accaatcaat ccggcgtcgc gcgcggctat tatgatgaag
     8521 cggcggtgca cacgctgcat gcctggatgg tttccgaggt gcggcagttc ggcgggacga
     8581 tcgatgacat ccgctattgc ccctatcacg agcaggcgac tgtcgcggct tatcgccgcg
     8641 caagcgactg gcgaaagccg gcgccaggga tgctgctgga tctgatgcgc gcgtgggaac
     8701 tcgagccgga gcgttgcatc ctgattggcg atcaggagac tgacatggcg gccgcgcgag
     8761 cagctggcat gaaagggcgg cgcttcgccg gaggcaatct ggcggatttc gtcaggccga
     8821 cattggaaag attcgcatgt cgttgctgag tggttttcgc gttgtgcagc ttggtcgcgg
     8881 tctggctgcg gcggtctgtg gtcggctgct ggctgatatc ggtgcggacg tgacctgcgt
     8941 tgatgcggac acttccaccc ctctcgcggc ctatctaaat cgcggcaaga cgatcggcac
     9001 acgggatgcg ttggcgtccg cgaatctcat tgtccgagaa agcgatgacg gagtggtaga
     9061 taccacggcc gcgcaggtgt tcatttcgcc gttcggccgc accggcgcaa aggcgaatgc
     9121 accggccagc gaccttacgt tgatgtatgc cagtggcatc gcgcggttgc tgaccggcca
     9181 ggtggacgat ctgaacgaag cgccgatccg tccgattgga gagcagtcgg cttttatcgg
     9241 tggacttgcc gccgcctgcg ctgggatgca cgcggcgcaa cagcacatgt cgggcgcgat
     9301 catcgacgtt tcgattgagg aagcgctggc gaccatggcg atgaccgagc tggccggcgc
     9361 cgcattgcat ggccgcagcc gctcgcgcaa gcgcatcacc gatggcaacg gcgcgacggt
     9421 ttgcatcctg cccgcgcgcg atggtttcac tgccatttct ccgcgcgagg acaggcaatg
     9481 ggcggcttgg ctgcaggcga tgggttcgcc ggcatggggc gaagacccgc gcttccggac
     9541 caaggctgat cgtgtcgcga actggaatgc gctgcatgcg ctgatgtcgg aatggagccg
     9601 ccggtacgac aagcagcgga ttgccgacac ggcgcaggcg gcgcgcgtgc cgagcttccc
     9661 gttgcgcgaa ctggctgagc agcttgattc gccacagctc gagcaccgaa attactggca
     9721 ggcgtgggat ggcgctaaag tgcccggatc gccgtttggc ctgacgatga cgccggcgca
     9781 gaggacggct gcggcgggtc cattgccgct gtctggcgta cgtgtgctcg atttcagttg
     9841 ggtgatcgcg ggaccgacga cgacacgata tctcgcagcg atgggagccg atgtgatcaa
     9901 ggtcgaggcg cctggccgtg gcgatcccgg tcgcgcgtcg gaactgcata ctgtgctggg
     9961 tcaggcgaag cgggcgatcg ttctggatct gaagcagccc gaagcggtcg atatcgcgcg
    10021 ccggttggcg gagcgttgcg atgtgctggt ggagaatttc gccaccggcg tgatggaccg
    10081 ccttggcctc ggtgcatcgg cgctgcggga atgcaatccc gggctgatct acgtttccgc
    10141 atcggggctg gggcgcagcg ggcctgagtc tcatgcggtc gcttatggga cgctgctgca
    10201 atgttatgct ggcttcgccg gactcaaccg ccatcccgag attgcgcctc gcgttggctt
    10261 tgcctggctc gatccgatgt gtgggttgat gctcgctttc atcaccgccg cggccatctg
    10321 gcggcgccgt gcgactggcg aaacggcgcg cgtggatttt tccatgatcg aagcgatgct
    10381 ctggacgttg gcgggaccgc tgctggcgac gcaacgcggg acaccgccgg cgccacaagg
    10441 caatcgctcc gatggccacc tgctgcacgg cgcctggcgt tgcgcgggcg atgatgcgtg
    10501 gatcagcatc gccgtcgcca acaatcagca gcaacagtgc ctgctcgatg tgattggtga
    10561 tgcatcgctt gaagcgtggc tggcgcaacg gacggctgcg gatgctgaag cgacgttgct
    10621 gagagccggc gtgccggctg ccgccctcgc gagctcgacc gatcttgtcg caagcgaaca
    10681 tctgcgcgcg cggggcttct gggatggtgg cttacccggg ttgccatggc gcgcaagctt
    10741 cggccgcagg tggggcgatg cgccgaagct tggtgcggat accgatgccg tgttggccga
    10801 ggtcctcgga tgtcaggagc cggatatccg tgccctgcgc gccaggggag cgttgggctg
    10861 aagccagcta ggtcagcagc cgttccggaa tacgcatgcc caatcgtagc ggtacgttcc
    10921 acacttcgcg gacctgccgc agggtgcgga agccggcgcg gagatacgtg ggcaatgcgc
    10981 gtggatggtc ggcggtgcag gtgttcaccg tcatgccgcg cgggttctcg cgccaggctg
    11041 tatctacggc gttgcgcagg aagggatagc ctacgccagc cccgatcgca tgcggcatca
    11101 ggccgaagta gctgagattg atgtccggcc agccgcgacc gtccagttcg tagaaccctg
    11161 ccggctcgcc attgctgtac aggacatgga tcgatactgc cgggtcgctc agcagcgccg
    11221 cgatttcgtc atccgccacg gtgcggcgga gccaccagac gtagtcggca cctactgtat
    11281 tatataggta acgatagaat cccaccgtgg gcgctgttgc gcgcacgacc tgaaagtccg
    11341 gcggcagtcc cggcgaggga acggccggag gccgatccat ccgcagaaac gtgaccgtca
    11401 cggtgactcg cgccaccggc tccatcttct tctctccctc gctggagcgc ttcagtcatc
    11461 cagggaagcg gcgcctgcac gcgaggtcag gttagactgc cgcccgagtt cttccaagcg
    11521 gttgcgccct gctatgtgac gacaattgcg gaagacacca gcgcacctat ggtcacgtta
    11581 ctggcgcctt gtgccagcat ccacaggtag taggtgccag cggtcgccgg tgcgtcgaaa
    11641 tactgccccc atccattggg ctggccgtcg gaaccggcca cgatgaggcc cgtcgtaggt
    11701 actgtcgaag agcttgctga ccaggcgaag gccgctccgg tcacggcggt aggcgccggt
    11761 gtggcggtga ggttcgcccc accggcaggc gcgacgcccg tgttgtggac ctgcgtggtt
    11821 gccatggcga ggttgcccgc agcgaccgtg gctccccagg tgctggccgt cgtggtggcg
    11881 gaccatgcgc cggcattccc cgatgcattc gtcgcctgga cttcgacatc gatcgcggcc
    11941 gcaccggaga gtccactgat gctgtacggg ctggtgacgc ctgaaatcgt tgtccaagta
    12001 ccggcccccg acgggctgta gcgaaggtta tagcctgcgg ccgccggatg gtcgccgtcg
    12061 gctgccggcg gcatccaggc aacagtaagt acgctgttgg tgccgtccgg tggtggtgca
    12121 acgaagctga tcgacggcgc atttggcaca gaagcaccag aggccgtgga cagcgaactg
    12181 gtggctgacc atgcgctggc gccggccgcg ttcaggcttt gcacctggac atcatatgcc
    12241 gcgccaaagg tcagtcctac taacaaatgt gggcgggtga cgttcgatag gactgtccag
    12301 gcattggcgc cggctaggct gaattgcagg ttgaacgcag tcgcggcgcc atgcgagctg
    12361 tcgacgggcg gaactgtcca ggtcgcggtc aggtcgcctc ccgcgccttg tgcgagggat
    12421 ggggctgacg ggatgttggg aaccagtgtc acgggggagg gggccgttgg cgcgttgacc
    12481 cgggacaggg gaagcatgac ggaatgggac cggacgcccg gcgttggctg atatatcggc
    12541 atgtgttgat acggccccgc tctctaagat caatcatggc aacgatcggg gctctccccg
    12601 ttattcgtca tttatactaa cgaatgatgg tccgggatgt caaactgaat ctttgtggtg
    12661 attcggccgt ggctggcagg agagggtcag tcgtccagga agctgcgcaa ctttcggctt
    12721 cggctcggat gcttcagctt gcggagggcc ttggcctcga tctggcggat gcgctcgcgc
    12781 gtcacgttga actgctggcc gacctcctag agcgtgtggt cagtgttcat gccgatgccg
    12841 aagcgcatgc gcagtacgcg ttcctcgcgc ggcgtcagcg acgacagtac gcgcgtggtc
    12901 gcttcacgga gattggcctg gattgcggcg tccagcggga tgactgccgc cttgtcctcg
    12961 atgaagtcgc ccagatggct gtcttcctcg tcgccgatcg gcgtttccag gctgatcggc
    13021 tctttggcga tcttcagcac cttgcggact ttctcgagcg gcatgccgag tttctcagcc
    13081 agctcctcgg gcgcgggctc gcggccgatc tcgtgcagca tctggcgcga ggtgcgcacc
    13141 agtttgttga tcgtctcgat catgtgcacc gggatgcgga tcgtgcgcgc ctgatcagcg
    13201 attgagcggg tgatggcctg ccggatccac catgtggcgt aggtgctgaa cttgtagccg
    13261 cggcggtatt cgaacttatc gaccgccttc atcaggccga tattaccttc ctggatcagg
    13321 tccaggaact gca
//
LOCUS       DATGEM010000244         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1166681, whole genome shotgun sequence.
ACCESSION   DATGEM010000244 DATGEM010000000
VERSION     DATGEM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166681"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VKQ27_07755"
     CDS             complement(<1..203)
                     /locus_tag="VKQ27_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HLY88863.1"
                     /translation="MKALLSRLSIDPYIASIVGMVALASLLPAQGVGFKVASAAGTGA
                     IALLFFLQGARLSTQAALAGAKH"
     gene            305..1042
                     /locus_tag="VKQ27_07760"
                     /pseudo
     CDS             305..1042
                     /locus_tag="VKQ27_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(1078..1830)
                     /locus_tag="VKQ27_07765"
     CDS             complement(1078..1830)
                     /locus_tag="VKQ27_07765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLY88864.1"
                     /translation="MTERIAKWLARAGIASRRDAEKLIADGKVRLNNTPVTHPATFVS
                     PDDLIVVNGAVVEPPDRTRLWRYHKPVGLVTTHRDPQGRPTVFERLPSNIPRVISVGR
                     LDLTSEGLLLLTNDGALARRLELPATGWLRRYRVRVHGFPDERRLAAVAKGITIDGVA
                     YGPIDAGLDSRKGDNAWLTVSLREGRNREVRRVMTHLGLTVSRLIRIAYGSFQLGTLP
                     RGAVEEVPARVLRDQLPPAKIASDSAATGPRA"
     gene            complement(1930..>2295)
                     /locus_tag="VKQ27_07770"
     CDS             complement(1930..>2295)
                     /locus_tag="VKQ27_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631166.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLY88865.1"
                     /translation="AVGGTLIQHIPDAIADALEHEQPNPRHEPGHPVSVRTGTLLHRI
                     VGATEMRVNSAHHQAVRDPGPHAVVNALAPDSVIEGVEDARYRFCLGVQWHPEFLIDP
                     GDRRIFDALIAACHCDDGE"
BASE COUNT          363 a          696 c          841 g          395 t
ORIGIN      
        1 caatgcttcg ctccggcgag cgccgcctgg gtcgatagtc gcgcgccttg caggaagaac
       61 agcagcgcga tcgccccagt gccggcggcg cttgcaacct taaagccgac accctgggca
      121 ggcagcagcg aggcaagcgc caccatgccg acgatgctgg cgatgtaggg gtcgatcgac
      181 agccgggaga gcagagcctt catggacgcg aataattgcg ctgcgcgcgc tcattgtgaa
      241 ccgtgcttga tgtgcttacg gtgatcgtta tttatgatga gcggaggcag tcgtgcttga
      301 cccggtgctg ttggaaagct tcctcgctgt cgcccaggcc cgcaacttca gcgaggccgg
      361 gcggcgcctg aacctccggc agtccacgat cagccagcac atccggaaac tggaacagga
      421 agccggccga cggctgttcg tgcgcgacac ccattccgtg gtgcccaccg cggacggcga
      481 ggcgatggaa ggcttcgcac gctccattct ggaggccaac gaacgcgccc accgctactt
      541 cgccggttca cagctccgag gccgggtgcg gttcggaacc tcggaggatt ttgtcacatc
      601 gcgcctgccg gaggtgttgg ctgacttcgt ccgcctgcac ccgctggtgg atctggcact
      661 gaccgtggag aagagcgccg ggctgtatca gatgctcgat gccggcgatc tggatctggt
      721 gctgtgcaag cgccgcgcgg acgacgaccg tgggcgggtg gtgtggcgtg atcgcctcgt
      781 ctggatcggc accgatgcag cgcggatcga gccagaccag ccactgccac tgatcttgta
      841 cacgccgccg agcatcactc gttcagcggc gctggagacg ctggaacgtg ccgggcgatc
      901 gtggcgcatt gtctgctcca gcagcagcct gagcgggctg cgcgctgcgg cgctcgccgg
      961 gctgggggtg accgtgcatg cgggcgggct gatgccggag ggactggcgg agatgccggc
     1021 cgcctatcgt ctgccggatc ttggggatgt cgagttcgtg gtgttgggag cgacgcgtca
     1081 ggcgcgcggc cctgtggcgg cgctgtcgga tgcgattttg gcgggtggca actggtcgcg
     1141 cagcacgcgg gctggcactt cctcgaccgc gccgcgtggc agcgtgccga gttggaacga
     1201 gccgtaggcg atgcggatca gccgcgacac cgtcaggccg agatgggtca tgacgcggcg
     1261 gacctcgcga ttgcgaccct cgcgcaggct gacggtaagc caggcgttgt cgcccttacg
     1321 ggagtccagg ccagcgtcga tcgggccgta agcaacgcca tcgatggtaa tgcctttggc
     1381 gaccgctgcc aggcggcgtt catcggggaa gccgtggaca cgcacgcggt agcggcggag
     1441 ccagccggta gcgggaagct ccagccggcg agcgagcgcg ccatcgttgg tgagcaacag
     1501 caggccttca ctggtcaggt ctaggcgacc gacgctgatg acgcggggta tgttcgacgg
     1561 aagcctttcg aagaccgtgg ggcggccttg cggatcgcga tgagtggtca ccaggccaac
     1621 gggcttgtgg tagcgccaca ggcgggtgcg gtccggcggt tccaccaccg cgccgttcac
     1681 cactatcaga tcatcgggcg agacgaaggt ggcgggatgc gtcaccgggg tgttgttgag
     1741 gcgaaccttg ccgtcggcga tcagcttctc cgcatcgcgg cggctggcga tgccggcgcg
     1801 tgccagccac ttcgcgatgc gttcagtcat gtgttgttcc agacgtgcag gccgatgccc
     1861 ttgttgccgg ccccaaaggc gtgggtggtc gcgccaagcg cgaccatgac ggtgaggtgt
     1921 ggcgccaggt tattcaccgt catcacaatg gcaggcggcg atcagggcgt cgaagatgcg
     1981 gcggtcgcca gggtcaatga ggaactcggg atgccactga acgccgaggc agaagcggta
     2041 gcgcgcatct tccacgccct caatcacact gtcgggggcg agcgcgttga ccacggcgtg
     2101 cggtccggga tcgcggactg cctggtggtg cgcggagttc acacgcatct ccgtggcgcc
     2161 gacgatgcgg tgcagcagcg ttccggtgcg cacgctgacg gggtggccgg gttcgtggcg
     2221 tgggttgggc tgctcgtgct ccagcgcgtc ggcgatcgcg tcgggaatgt gctggatcag
     2281 cgtgccgccg accgc
//
LOCUS       DATGEM010000245         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_738997, whole genome shotgun sequence.
ACCESSION   DATGEM010000245 DATGEM010000000
VERSION     DATGEM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738997"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2500
                     /locus_tag="VKQ27_07775"
     CDS             <1..2500
                     /locus_tag="VKQ27_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88866.1"
                     /translation="LEPRIARCSSDDEPQPGAILALKVLDPAVGSGHFLVEACRYLAD
                     ALYAACRRCDELAAIADGRGLPARAELLRQRVATLPASAGTLLAYLPGRAGEGYASGV
                     SATRALAICRRLVAVRCLYGVDSNPLAIELAKLSLWLESYAEGLPLTFLDHRLVHGDS
                     IGGPLVGSLATLPVSREPLHPDLVTELGQRVDTAIDAARREVRALEVSVGVDAVDVAL
                     KAAARQRLDAALHVLRLLVRSWSGAVMCAQDDDAWLSLARRVAATGRWPASPNEHQAQ
                     MLEAGGLALPWDLTFPDVPDGFDAVISNPPWDIVQPNTSSFLAEFDLAVLDAQDNTAM
                     RHIQHHLLSEPSVARAWLAHKETFARRHRIVGRIYDHQKLGADGVAIGGKLDMYRVFA
                     ERMVQLAARDGAIGMIVPSAFHANEGASGVRQLYLEHTSLETCLSFENREKHFDIDGR
                     FKFALAIARKPGPTRTIRCGFYLTNLAQITETGRVITYDRDFVAASGGPHATFLELRH
                     RDDMALVRRMLQDHQPFRFWSGTAGVTLSRELHMTDDARNFTLAADVPPDVLHQYLPL
                     HEGKTIHQFSDRWGTGPRYVVAASRLAHKPQTVESARYYRCACREVAGATNERTVIAA
                     MLPPRVLCGHTISVERSPAQRPNASALALVGVMNSFCFDWLLRQKAAAHVSIYILAEL
                     PMPDLASDAERFVAHGGLRLCCNHRGFARLWGEQLGSAWSETGPRRSWPAIADEPERE
                     QLRAAMDAVVAYGYGLDHAQFERVLASFTHRSHPDAATRRLAAFDELASQGLQDFCHA
                     HDPYADIPMVTGRARPVINLVATRDLPVGAAAGV"
BASE COUNT          459 a          805 c          849 g          507 t
ORIGIN      
        1 gttggagccc aggatcgcgc gttgctcctc cgatgatgag ccccagccag gtgcgatact
       61 ggctttgaag gtgcttgatc cggccgtggg cagcggacac ttcctggtgg aggcgtgccg
      121 ataccttgcc gacgcactct acgccgcgtg ccggaggtgc gatgaactcg ccgcgatcgc
      181 cgatggcagg ggcctgcccg cacgcgccga attgttgcgg cagcgcgtgg cgacgcttcc
      241 cgcatccgcc ggcacgctgc tggcctatct gcctggtcga gccggcgaag gctatgcaag
      301 cggcgtgtcg gcgactcgcg cgctcgcgat ttgccggcgt ctcgtagctg tgcgttgcct
      361 gtatggcgtc gacagcaatc cgcttgcgat tgagctggcg aagctgtcgc tgtggctgga
      421 atcctatgcc gagggactac cgctgacgtt cctcgaccat cgactggttc acggcgattc
      481 gatcggcggc cccctcgtcg gatcgctggc gacgctgccc gtgtcgcgtg agccactgca
      541 tccggatctc gtgaccgaac tcggccagcg cgtagatacg gcgatagatg cggcacgaag
      601 ggaagtacgg gcgctggagg tgtccgtcgg tgtcgacgcg gtcgatgtcg cgctgaaagc
      661 ggcagccagg caacggctcg acgcggcgtt gcacgtacta cgactgttgg tgcgttcgtg
      721 gagcggtgcg gtcatgtgcg ctcaggacga cgacgcatgg ttatcgctgg ctcgtcgtgt
      781 cgcggcgaca gggcggtggc cggcatcgcc gaacgaacac caggcgcaga tgcttgaagc
      841 aggcggtctt gcgctgccct gggacctgac attccccgat gtgccagacg gctttgatgc
      901 tgtgatcagc aatccgccct gggatatcgt acagccgaac acgtcgagct tcctggccga
      961 gttcgatctc gctgtactcg acgcgcaaga taacacagca atgcggcata ttcagcatca
     1021 cctgctgagc gaaccgtccg tggcgcgcgc gtggcttgcg cacaaggaaa cattcgcgcg
     1081 gcgccaccgt atcgtcggtc gtatttacga ccaccaaaag ctcggcgccg acggcgtcgc
     1141 tatcggcggc aagctcgata tgtatcgtgt attcgccgag cgaatggtcc aactggccgc
     1201 tcgggatgga gctatcggca tgatcgtgcc ttcggcattc catgccaatg agggcgccag
     1261 cggcgtccga cagctgtacc tggagcacac atcgctggag acctgcctgt cattcgagaa
     1321 ccgtgagaaa cacttcgaca tagacggccg tttcaagttc gctcttgcga tcgctcggaa
     1381 accaggacca acccggacca tacgttgcgg gttctacctc accaatctgg cacagattac
     1441 cgagaccggc cgtgtgatta cctatgatcg cgacttcgtg gcggcgtcgg gtggccctca
     1501 tgcaaccttc ctggaactgc gccatcgcga tgatatggcc cttgttcgcc ggatgttgca
     1561 ggatcatcag cccttccgct tctggtcggg aacagctggt gtgacgctga gtcgggaact
     1621 gcacatgacc gacgatgcgc gcaatttcac gttggccgcc gatgtgccgc ccgatgtact
     1681 gcatcagtat ttgcccctgc acgaaggcaa gacgatccac cagttcagtg atcggtgggg
     1741 caccgggccg cgttatgtcg tcgccgcgag ccgactggca cacaagccgc aaaccgtgga
     1801 aagcgcgcgc tactatcgct gcgcatgccg tgaggttgcc ggagcaacca acgagcgtac
     1861 cgtgatagcc gccatgctac cgcccagagt gctgtgtggc cacacgatca gcgtcgagcg
     1921 gagccccgcg cagcgcccga acgcctcggc actcgcgctg gttggcgtga tgaacagctt
     1981 ctgcttcgac tggttgctgc ggcagaaggc ggctgcgcat gtcagcatct acatcttggc
     2041 cgagctgcca atgccggacc tggcgtcgga tgcggagcgc ttcgtcgcgc atggcggtct
     2101 gcggctgtgc tgcaaccatc gaggctttgc gcggttgtgg ggtgaacagc ttggcagtgc
     2161 atggtctgag accggaccgc gccgctcctg gccggcaatt gcggacgaac cggagcgtga
     2221 gcagctacgc gccgccatgg atgcggttgt cgcttacgga tacggcctcg atcatgcaca
     2281 gtttgagcgc gtgctggcga gctttaccca tcggtcgcat ccggatgccg ctacgcggcg
     2341 cctggcggca ttcgatgaac ttgccagcca gggcctccaa gacttctgtc atgcccacga
     2401 tccttacgcc gacattccaa tggtgaccgg acgggcgcgt ccggtcatca acctggtggc
     2461 aactcgcgat ctaccggtcg gcgccgccgc gggcgtatga tcatggccaa gaacaagggg
     2521 aaactttggc catggaagcc gaaagcttcg attatattgt cacgggcgct ggttcggcag
     2581 gctgtgtcgt cgcagcgcga ctgagcgaat ccggcaagca
//
LOCUS       DATGEM010000246         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_21332, whole genome shotgun sequence.
ACCESSION   DATGEM010000246 DATGEM010000000
VERSION     DATGEM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21332"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VKQ27_07780"
     CDS             <1..596
                     /locus_tag="VKQ27_07780"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY88867.1"
                     /translation="PKCSGVEDLGTLNHHLEALEAAAGAPIGSIGVVAIVTETAASVF
                     GLGTYAPVASRLLALCFGAEDLSGDLGINPRYSDGTYPAPVVFARGQTLVAAGALGVP
                     AIDTPYPDYTDPAGLKREADAAAADGFAGKILIHPAQIDVANAAFTPPDEQVRWAEQV
                     HAAFTDNPDRGVFALDGKMIDRPHWKLAQRILAAAGR"
     gene            complement(659..1585)
                     /locus_tag="VKQ27_07785"
     CDS             complement(659..1585)
                     /locus_tag="VKQ27_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLY88868.1"
                     /translation="MRIGMVTDSLAHLSLDDLLHTATELGIEMLEFPCGNWSPAPHLN
                     LDAMLASAQMRTEFQARLRDHNLSLSALNCSGNPLHPGDIGRQHQIVTSKTIRLAGLL
                     GVDRVVLMSGCPGGPGDANANWVTTAWPPEAARVLAWQWDEVLIPYWKGLVAEARAAG
                     VSKLCLELHGQQNVYNVATLFRLRDAVGDTVGANLDPSHLFWMGADPIAATRVLAPAI
                     YHVHAKDTRIDRANASINSLIDTTPNDRLLDRAWSYVTLGYGHDLLWWREFCSILRLV
                     GYDDVLSIEHEDQALPPLEGVRRSVDLLRNVI"
     gene            complement(1576..>2176)
                     /locus_tag="VKQ27_07790"
     CDS             complement(1576..>2176)
                     /locus_tag="VKQ27_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY88869.1"
                     /translation="AGAIGRLELLTIFSKDPGPPPISYVQTSGGLFRDCMIHDLDMAR
                     FLLGEEPVELHASASCLVDPAIGAVGDVDTAVATLRTTGGKLCQISNSRRATYGYDQR
                     IEAHGEKGLLRAGNVTATTVEFASGSGFASEPALPFFLERYAEAYRRELDAFITAVAA
                     GQAPQPSGEDGLKALLLADAATRSAQTGETVRLGAERCV"
BASE COUNT          370 a          651 c          768 g          387 t
ORIGIN      
        1 tgccgaaatg cagcggcgtg gaagacctgg ggacactgaa ccaccatctg gaggcgctgg
       61 aagctgcggc cggagcgccg atcgggtcaa tcggggtggt ggcgattgta acggagaccg
      121 cggcttcggt gttcgggctg ggaacctacg cgccggtggc gtcgcggctg ctggcactgt
      181 gtttcggggc tgaggatctt tcgggggacc tggggatcaa tccgcgctat tccgatggga
      241 cgtatcccgc tccggttgtg ttcgcgcgcg ggcagaccct ggtggcggct ggcgcgctgg
      301 gcgtgccggc gatcgatacc ccgtatccgg attacaccga cccggcgggg ctgaagcgcg
      361 aggcggatgc ggccgccgca gatgggtttg ccgggaagat cctgatccat ccggcgcaga
      421 tcgacgtggc gaatgccgcg ttcacgccgc cggatgagca ggtgcgttgg gccgagcagg
      481 tgcatgccgc attcactgac aatccggatc gcggggtgtt tgcgctggat gggaagatga
      541 tcgatcggcc ccactggaag ctggcgcagc ggatcctggc cgcggcgggg cggtagctac
      601 cgaagtggcc gtctgccgtc gatgcgtgac ttgcgatcct gcaccgcgtt cccacccttc
      661 agatcacgtt gcgcagcagg tccacggaac gacgcacccc ttccaacggg ggcagtgcct
      721 ggtcttcgtg ctcgatcgat aacacgtcgt catagccgac aaggcgcagg atggagcaga
      781 attcgcgcca ccacagaaga tcgtggccgt agccgagcgt gacatagctc caggcgcgat
      841 ccagcagacg gtcgttgggc gtggtgtcga tcaggctgtt gattgaagca ttggcgcgat
      901 cgatgcgcgt gtccttggcg tgaacgtgat agatcgcggg agccaggacg cgtgtggccg
      961 cgattggatc agcacccatc caaaaaagat ggctgggatc gagattggcg cccacggtgt
     1021 cgccaacggc gtcacgcagg cggaacaagg tggcgacgtt gtacacgttc tgctggccgt
     1081 ggagctccag gcatagcttg ctgacgccgg cagccctggc ttctgcgacg aggcctttcc
     1141 aatacgggat cagaacttcg tcccactgcc aggcgagcac gcgcgcggct tccggcggcc
     1201 aggcggtggt cacccagttg gcgttggcat caccggggcc gcctggacag ccggacatca
     1261 ggacgacgcg gtccacgcca agcagaccgg ccagccggat ggtcttggac gtgacgatct
     1321 ggtgctggcg gccaatgtct cctgggtgga gggggttgcc ggaacagttg agcgcgctga
     1381 gggagaggtt gtgatcgcgg agcctggcct ggaactcggt gcgcatctgg gcgctggcaa
     1441 gcatcgcatc gaggttgaga tgaggcgccg gcgaccagtt gccgcagggg aactccagca
     1501 tctcgatgcc gagttccgtg gcagtatgga gcaggtcgtc gagcgagaga tgggccaggc
     1561 tgtcggtcac catgcctata cgcatcgctc tgctccaagc cgtaccgttt cgccagtctg
     1621 cgccgaacgc gtggcggcat cggccaacag cagggctttc aggccatctt ctccgcttgg
     1681 ctgcggtgcc tggcccgccg ccacggccgt tatgaatgca tccagttcgc gccggtatgc
     1741 ctctgcgtag cgctccagga agaacggcag cgccggttcg ctggcgaaac cggatccgct
     1801 ggcgaactcc accgttgtcg cggtgacatt gccggcacgt agcaggccct tctcgccgtg
     1861 ggcttcgatg cgctggtcgt aaccgtaggt agcacggcga gagttggaga tctggcacag
     1921 cttgccgccc gtggtgcgca gcgtggctac ggcagtatcc acatcgccca ccgcgccgat
     1981 cgccgggtcc accaggcatg atgcggaagc gtgcagctcc accggttctt cgcccaacag
     2041 aaagcgcgcc atgtcaaggt cgtggatcat gcagtcgcgg aacaggccgc ccgaggtctg
     2101 aacatagctg atcggcggcg gccctggatc tttgctgaag atggtcagca gctcgagcct
     2161 gccgatcgcg ccggca
//
LOCUS       DATGEM010000247        18000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_567526, whole genome shotgun sequence.
ACCESSION   DATGEM010000247 DATGEM010000000
VERSION     DATGEM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 18000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18000
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567526"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VKQ27_07795"
     CDS             <1..622
                     /locus_tag="VKQ27_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HLY88870.1"
                     /translation="AWVDSLLIDHAVFCLVWSNFAAVAPGRLYRSNHPTPARLIALTR
                     RYGLKTLINLRGRTGNGSDALSRAAAEELGLEFVDMPLESRGAPQRDRILRLHDIYQH
                     MHAPALIHCKSGADRAGLAAGLFVMFQGGTARQALQQLSLRFGHIRQARTGILDAFFH
                     HYAGVAEGRKPFLDWVREDYDEAALRRDFHANGLASFINDWVLVHE"
     gene            640..1509
                     /locus_tag="VKQ27_07800"
     CDS             640..1509
                     /locus_tag="VKQ27_07800"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HLY88871.1"
                     /translation="MIERLPLRAWNGPFDTEVCTQAVDALESGRVLLAELPFGMSPNE
                     TFLLSPAIMGSERKNISYDPPTGQLANTALAGAQADCLKTMLRRFGDAAETLLRDLLP
                     GYAADLQRARTSFRPAEIAGRDYSPRHDDRLLHVDAFPSRPMHGRRILRLFCNVAPDG
                     VPRAWRVGEPFTDFARQFLPRTGNAVIGSAWLLERLGITKGRRTEYDRIMLRLHDTGK
                     LDSAYQSTAPRTDIAFSAGSTWICYTDQVLHAALAGHCALEQTFHLPISAMANPERSP
                     LRVLEQLAGRSLT"
     gene            complement(1538..1696)
                     /locus_tag="VKQ27_07805"
     CDS             complement(1538..1696)
                     /locus_tag="VKQ27_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88872.1"
                     /translation="MRAEDARQLADTMVDAGARHTMLLIAAGYEKMARRAALMHHMDL
                     PTALVDEC"
     gene            2035..3378
                     /locus_tag="VKQ27_07810"
     CDS             2035..3378
                     /locus_tag="VKQ27_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HLY88873.1"
                     /translation="MPTNPADSPILGTLYGSDAMRAVFDERALFQRMLDVEAALARAQ
                     ARLGVIPSEAAQSISRAARLENLNISELAESARTAGAPVVGVVAGLSRAAGDAGAWTH
                     WGATTQDIVDTAIVLQIRDGLGLIRASLRELVSALVAQSHRHRRTVMAGRTYLQQALP
                     TTLGLKCAIWAQPLIGHVHRLDALRSRVEQVSFGGAAGTLASLGSQGIAVMEGLAAEL
                     DLAAPAAPWHVNREGFAETVAFLGLICGSLAKFATDIILLTQTEVGEVAEPYFAGRGA
                     SSTMPQKRNPIASTYIVAAARNVHALVPVMLGTMAADQERPTGAWQAEWLALPQAFVL
                     THGALDHAGSIARGMVAAPASMQRNLDLTHGLIVAEAVMMGLAPQLGRAEAHHAVKHA
                     CDVALTEDISLADALAREPALAGKLNRAEIERLTDPANYLGSTDAFIDRVLALAT"
     gene            3796..4899
                     /gene="ugpC"
                     /locus_tag="VKQ27_07815"
     CDS             3796..4899
                     /gene="ugpC"
                     /locus_tag="VKQ27_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HLY88874.1"
                     /translation="MAEVSCRKVVKNYEGGVQAVKGIDLEIADQEFVVLVGPSGCGKS
                     TTLRMIAGLEEISDGEIWIGGDVVNDVPPRDRDIAMVFQNYALYPHMSVFDNMAFGLK
                     LRKFPKDEIKRRVDEAARILDIGMLLDRKPRALSGGQRQRVAMGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRTEIKKVHQTVRTTTVYVTHDQVEAMTLADRVVVMNHGVIEQVGA
                     PQELYHNPTTRFVAGFIGSPAMNFLPVRVEDGTGGGLAVRVTDQIKLPIPPARNDRYG
                     KYKDREMVLGLRPEHLFEYHENGRPGWERVELPVDVVEPMGMETMVHFFIGHEPVCAR
                     VDPATHAEPGEILPLTADMGQMHLMEPDTGRVV"
     gene            complement(4918..6021)
                     /locus_tag="VKQ27_07820"
     CDS             complement(4918..6021)
                     /locus_tag="VKQ27_07820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY88875.1"
                     /translation="MDTYQFDAVVIGAGIAGSTAAAALSPDCRVALIEAEAAAGYHTT
                     GRSAAMWILTYGPHDVQHLTGLSRSFLVSPPAGFTETPLLSQRSEVLVAPENQRAELE
                     AVLGGGIRPLQLDDLRAMIPAMRPDYAVLAAIEEDAFDIDVAALHQGYLRQLRAHSGV
                     LALRNRAGRIERRDGAWDVETSSGDVFRARTVVNASGSWGDEVAAIAGVAPLGLKPCR
                     RTGVIIDPAPWQVEHWPMVCDIGETWYARPEARTRLMVSPADKTPMYPHDVQPDEFDI
                     AVAIDRMQQALDIDVRRVERSWAGLRTFTPDGSLAFGWAPDAAGFFWCVGQGGYGIQT
                     SPAAGRLCADLILARDPGEAGEIVATISPARFS"
     gene            6350..6787
                     /locus_tag="VKQ27_07825"
     CDS             6350..6787
                     /locus_tag="VKQ27_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY88876.1"
                     /translation="MALKRMDNVGIVVEHLEETIDFFRELGLELEGRATIEEEWAGRV
                     TGLGDQRVEIAMMRTPDGHGRLELSRFLTPPAIADHRNAPVNALGYLRVMFAVDDIDE
                     TLERLRRRGTQLVGQVVQYKDAYRLCYIRGPEGLLIGLAQELS"
     gene            6893..7660
                     /locus_tag="VKQ27_07830"
     CDS             6893..7660
                     /locus_tag="VKQ27_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HLY88877.1"
                     /translation="MQSRITGTTMPVLEIGLEPGDVLVAPPGELSWMTANVQMRTTTA
                     TAGARGLWGAITRAVSGGGLFMTEFATAGGQGAVAFAAKVPGQIMPIDVQPGRGYLVH
                     RHGFLCGMQGIELGMALQRSLGGGIFGGEGFVLQRLTGNGQAWVELGGEIVTHELGAG
                     ETLLVHPGHVGMFEESVSFDITMIRGITNAIFGGDGLFLVQLTGPGRIWLQTLTMPNL
                     AHALMPYLGRQEPASTAQTVEAGAAGAVLRDIFGGQR"
     gene            complement(7785..8486)
                     /locus_tag="VKQ27_07835"
     CDS             complement(7785..8486)
                     /locus_tag="VKQ27_07835"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HLY88878.1"
                     /translation="MTNISQVAKDRGGKRPVQGYARGDATRLHIIETAVEVFGTHGFE
                     DASTRLIARRAGVNLAALHYYFGGKRELYRACADHLAGMGEARVAPFLAMMEQPGASR
                     EALLELLHRLLDAFADRLVGPRDPPALVAFFLREQIAPTEAYEILHRRVMAPLLGACA
                     SIVGRLIDQPADAEETVLLTLAILGPLMVFQRAREVSLRALHWPDFEGERLVRVKALV
                     WQQAQTMVAGRRGAA"
     gene            8526..9995
                     /locus_tag="VKQ27_07840"
     CDS             8526..9995
                     /locus_tag="VKQ27_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLY88879.1"
                     /translation="MRNMRALMSLVALLAGCTVGPDYHPPDTPMPARFSGPASAATAD
                     IAQWWHSFGDPELDHLIEQAMRDNPDVQTAASRVREARLQEIVAGAPLWPSLRADDQA
                     AHTHLSGNAISLGNLGTLTGGAIPSGGSSSLSSLGLSGTSFNTYQLGFDASWELDLFG
                     KTRRSVEASRDSTGAAVWDRRDTQVSLAAEVANTWFALHAAQARLDVAKGELARQQAL
                     MAVLRTRQSTGFVTGLDVAQQDTQVTAAAAAVPPFQAEIDARRHALAVLVGEAPETLD
                     LPATGAGSATPPGIPPGLPSDLLRRRPDIRSAERQLAAATANIGVAVADYYPQITLTA
                     SPSLISTALSNLLTWGSRNLSLSAGLVWPLFNAGTTKAKVGIANEHQQQALLAYRKTV
                     LTALKDVEDALSQYQSDRDRQAALQRSLNAAGNAAAVAHGQYRAGLIPFSTVLTTEAA
                     LYSARDQSAQANAALNQDLVALYKALGGGWSEETTEAAR"
     gene            9992..10942
                     /locus_tag="VKQ27_07845"
     CDS             9992..10942
                     /locus_tag="VKQ27_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLY88880.1"
                     /translation="MNRVRQILMILIAVGVGGWLAWRAWGPHPEDRVALSGYIEGEYL
                     YLSAPISGFVARVDVARGDRVAAGQKLFAMDDAPLRAQRDQAAANVTQAQAQIGSAQA
                     KLAQSRATAEATQAQATNAAEDLKRYRSALRVNAETVSRQQVDTAVATAANSAGQADA
                     ARSDVAAQEAQIAAAKAQAAAQQAALADVAARLAQLEPHAPAAARAQDVFYQKGEWVA
                     ENQPVISLLPDDKVKVRFFVPEAQVARYRPGQTVHFDCDGCATGLTARINYVSTQPEY
                     TPPIIYSRSTRDKLVFLVEALPADGRNLTPGLPVDVEPLR"
     gene            11100..12005
                     /locus_tag="VKQ27_07850"
     CDS             11100..12005
                     /locus_tag="VKQ27_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY88881.1"
                     /translation="MVLAIDVHDLNKSFGAHQVVRDVAIQVEAGHICGFLGPNGSGKT
                     TTLRMLCGLLTPDTGAGTCLGFDILKQTDQIKRNTGYMTQRFSLYEDLTVEENLDFIA
                     RMYGLDRRRERLQQALGRLGLADRRDQRAGTLSGGWKQRLALAACVLHQPRLLLLDEP
                     TAGVDPKARREFWDELHRLAGEGITVLVSTHYMDEAERCHDVAYIVGGRLIGRGTAEE
                     LVEQAGLITFLAEGARADRLAEELKGHPGVVMATPFGQDLHVSGTDRAALENAIAPHR
                     HEPYRWTETRPTLEEVFIYMMDADA"
     gene            12002..13126
                     /locus_tag="VKQ27_07855"
     CDS             12002..13126
                     /locus_tag="VKQ27_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY88882.1"
                     /translation="MIRFSFGRVLAVLVKEFRQMMRGQPSVILSIPIMQLLLFGYAIN
                     DNPHHLPTGVYIQDQGEFARSILAAMRNTGYFDIVATARNSAELDRLMALGKITFSVT
                     IPGDFTRRVIRHDNAQILVEADASDPTATGSAIAALAALPTTALARDLIGAVTPAATS
                     APFTAVVHSRYNPEAITSYNIVPGLLGTVLSMTLVFMTAQSLAKEREVGTLEGLLATP
                     VRPVEVLIGKLAPYVGIGILQTGIILLLARALFDVPEAGGWDALAIGVVLFIVGSLAL
                     GFLVSTFARTQQEAQLIAQFYFMPSFLLSGFFFPFAGMPGWAQAIGSLVPVTHFLRIV
                     RGSLLKGQGIADAWPSLAALMLFVLGVSVLAILRYRTTLD"
     gene            13194..14840
                     /locus_tag="VKQ27_07860"
     CDS             13194..14840
                     /locus_tag="VKQ27_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88883.1"
                     /translation="MDYPYDVGPYSRTVTTTSPDAQRWFDRGLNWCFGFHHEEAIRCF
                     EKALEADANCAMAHWGIGYAAGPNYNFPWELMDPAGKAATLGRAYDATRAALALAPNV
                     SAPERALIEALPSRYPQRDPIEDQRPWNNAFADAMRLVHQAHPDDLDIRNVFVEAIMN
                     RTPWRMWDLRTGATAPDAGTTEAREVLETAFSTLPGAMSHPGLLHLHVHLMEMSPNPE
                     AALVTGDRLREISPDMGHLTHMPTHIDIQCGHYRDAMLWNQRAIIADRKFYDRVGPMN
                     FYSGYRIHNYHFAAYGAMFLGQYEPAIAAANELVETMPEDFLRIPSPPMANFFESYVA
                     VRLHVMIRFGKWHEIIAQTLPADPELYCNTVAMTHYAKGVAHAALGDVPAAEAEQALF
                     RDAAKRVPDTRYIHNVKCVSLLAIAEQMLAGEIAYRRGEHDAAFAHLRAAISLEDDLP
                     YDEPWGWMQPVRHALGALLLEQGRAAEAEVVYREDLGLGGTLPRAQIHPDNVWALRGL
                     LDCLERRGERAEATLIRQRVTFAEARADVPVAVSCFCARG"
     gene            complement(14853..15137)
                     /locus_tag="VKQ27_07865"
     CDS             complement(14853..15137)
                     /locus_tag="VKQ27_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88884.1"
                     /translation="MIAFRILFGIDALAAAVVVFFFLWGLSDGTVSSFNILLWLAMLG
                     GVGGVPGGGVWLRANGHLRLANGVLLLLALPATLIALFFLLLIVAQPRWN"
     gene            15242..16666
                     /locus_tag="VKQ27_07870"
     CDS             15242..16666
                     /locus_tag="VKQ27_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY88885.1"
                     /translation="MVDLVVRGDTVVTPQGVGAYDIAIAGERIVAMAASGTFPIPDGA
                     RLIDATGKIVMPGGIDPHVHCKWFMPHPDGTAADTDPPDVVGRAALHGGTTTMIDFTR
                     ANQDPSLQKAIEKREQDWKGHSPCDYAQHIMVEGALPIDLPGQLAEAIQAGFATVKIF
                     TTDITPSRKGRMVDFGDIWEVFQVLKASGGLGVIHSEDNDIVMHMYGKLIREGRTGFT
                     NMAEVHNTLSEDISFRRVIRLAEKVGATLYMMHTSAASGVSAIREARARGVSIYGESL
                     HQYMLYTNEDYKRQNGQIYHTYPSLKSAEDQAALWSGTLDGSINCVATDEICCTLAKK
                     LQGSRIDDTTGGNAGVEPRVSLMYTEMVQNRRYSLSRFVDLVSTNAAKIMGLYPRKGA
                     LAAGSDADICVLDPGDNRVIRAQDLHEADYSPWEGRKMAAWPCLTVLRGKVVVENGAW
                     KGAVTDGKWQHRKVASEMLAGPML"
     gene            16755..17030
                     /locus_tag="VKQ27_07875"
     CDS             16755..17030
                     /locus_tag="VKQ27_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88886.1"
                     /translation="MTDAELRALLLDCLSLWGVKGRVEAADPGMRIIAGTTTLAVERA
                     PADMRPVRWLLHRPNRAPRVAPSIGALLTALRNALGAEAGNKLRVGT"
     gene            17027..>18000
                     /locus_tag="VKQ27_07880"
     CDS             17027..>18000
                     /locus_tag="VKQ27_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88887.1"
                     /translation="MKQDERGLPLSTDSTEAVHSFDRAVEHFLKFHADTMALVGRALA
                     ADPGFVMGHCLKAYLLLIAANPANRAQIDATLASAQAGAANITTRERMHLAAAEHWHR
                     GALDESFRIWGEILDAHPTDLLTFRISDTIWFRHGQTQPILTQADRVTPRWSADLPGY
                     DCAQTIWAFAHEEVGDTKNAERAIDAALERDATNYFAHHVKAHIMDTDCRAREGSDWL
                     GSQVPNWSLGNNLIHHLWWHRALLRLDLGEVDAVMASYDQDIRNFDAPMTKATPDHYV
                     DLQNAAALLWRLEQAGLDVGNRWEELADKAEARIGEAGHLLMVPHLMMA"
BASE COUNT         3329 a         6047 c         5602 g         3022 t
ORIGIN      
        1 tgcgtgggtc gattcgctgc tgatcgatca cgccgtgttc tgcctggtct ggagcaactt
       61 tgcggcggtc gcgccggggc ggctgtatcg gagcaatcat ccgacaccgg cgcgattgat
      121 cgctttgacg cggcgctacg gtttgaagac gctgatcaac ctgcgcggcc ggaccggcaa
      181 cggttcggac gcactgagcc gggcggcggc ggaagagctg gggctcgaat ttgtcgacat
      241 gccgttggag agccggggtg cgccgcaacg cgatcggatc ctgcgattgc atgatatcta
      301 ccagcacatg catgctcccg cgcttataca ctgcaaatcg ggtgctgatc gcgccggatt
      361 ggcggccggg ctgttcgtga tgttccaggg cggcactgcc cgtcaggcgc tgcagcagct
      421 ttcgttgcgg ttcggccaca tccggcaggc gaggaccggc atactggacg catttttcca
      481 tcactacgcc ggtgtggccg agggccggaa acccttcctc gactgggtac gtgaggacta
      541 tgatgaagca gcgctgcgcc gggatttcca tgcaaatggg ctggcaagtt tcatcaatga
      601 ctgggttctg gtgcacgaat agcgatggat gctgatcacg tgatcgaacg cctgcccttg
      661 cgggcgtgga atggcccatt cgatacagaa gtatgcacgc aggcggtcga tgcgttggaa
      721 tcaggccgcg tgctgttggc ggagctgcca ttcggtatgt cgccgaatga gacgtttctg
      781 ctgtcgcctg cgatcatggg cagtgagcgg aagaacatca gctacgatcc acccaccggc
      841 cagcttgcca acaccgcgct cgccggtgct caggccgact gcctgaagac catgctgcgc
      901 cgctttggcg atgcggccga gacgttgctg cgagatctgc tgccgggcta cgcggccgat
      961 ctgcagcgtg cgcgcacgag tttccgtccc gcggaaatcg cgggccgcga ctattcgccg
     1021 cggcacgacg atcggttgct gcatgtcgat gcgttcccca gccgcccgat gcacggcagg
     1081 cgcatcctgc ggctgttctg caacgtggca ccggatggcg tcccgcgtgc ctggcgcgtc
     1141 ggcgaaccgt tcaccgattt cgcgcggcag ttcctgccgc gaaccggcaa cgcggtcatc
     1201 ggcagtgcat ggttgctgga gcggttgggc atcacgaagg gacggcgcac cgaatacgac
     1261 cgcatcatgt tgcgcctgca tgacaccgga aagctggact cggcatatca atcgacagcg
     1321 ccacgaaccg acatcgcgtt ttctgctggt tcaacctgga tctgctacac tgaccaggtg
     1381 ctgcatgccg cactcgccgg ccattgcgcc ctggaacaga catttcattt gcccatctcc
     1441 gccatggcaa atccggagcg ctcgccattg cgcgtcctgg agcaactcgc aggacgatca
     1501 cttacctgac cccgctgccg cccagcgaac tcaggcgcta acactcatcg accagcgcag
     1561 tcggcagatc catgtggtgc atcagcgccg cgcgccgcgc catcttctcg tatccagccg
     1621 cgatcagcag catggtatgg cgcgcgccgg cgtcgaccat tgtgtcggcg agttgccggg
     1681 catcttctgc tctcaacaac cactccgccg gggttgaggg accggccatg ccgttttgat
     1741 agctatcagt attcggcaaa ccgaatagca aacgcgtgat acttcgtgag caataacgca
     1801 gaattggcca atatccgccg cgccccgtga cacgttcact taaagctgaa ccaagccgcc
     1861 gggctggtga ccagcaatca agccatggcc cgggagctac agctagcggc acggcttcgc
     1921 cccgaacctt cttgccgaat cgcaaattgc tcagatcttc aaggtctcgg gcctgggatt
     1981 actgccgaca cggtgcgata cacacgggtc aatattttcg ggcgagcaat ccccatgccc
     2041 acaaaccccg ccgacagccc gatcctgggc acgctctacg gcagcgacgc tatgcgcgcc
     2101 gtgttcgacg aacgcgccct gttccagcgc atgctggatg tcgaggccgc tctcgccagg
     2161 gcgcaggcca ggctcggcgt catcccatcc gaggcggctc agtccatcag ccgtgccgcg
     2221 cgcctcgaaa acctgaacat cagcgaactt gccgaaagcg ctcgcaccgc cggtgcgccg
     2281 gtcgttggag tggtcgccgg attgtcgcgc gccgccggcg atgccggtgc ctggacgcac
     2341 tggggcgcca caacgcagga catcgtcgat accgccatcg tgctgcagat ccgcgacggc
     2401 ctcgggctca tccgcgcttc gctgcgcgag cttgtgagcg cactcgttgc gcaatcccat
     2461 cgtcatcgcc gcaccgtgat ggcggggcgt acctatctgc agcaagccct gcccacgacg
     2521 ctcggattga aatgcgcgat ctgggcgcag ccgctgatcg gccacgtcca ccgcctcgac
     2581 gcgcttcgct cccgggtcga gcaagtcagc tttggcggtg ccgccggcac acttgcttcc
     2641 ctcggcagcc agggcattgc cgtcatggag gggctggccg ccgaactgga tttggccgcc
     2701 cccgctgccc cctggcatgt caaccgggaa ggcttcgccg agacggtggc gttcctcggc
     2761 ctgatctgcg gcagcctcgc aaaattcgcc accgacatca tcctgttgac gcaaacggaa
     2821 gtgggcgagg tggcggaacc ttacttcgcc ggccgtggcg cctcctccac catgccgcag
     2881 aagcggaacc ctatcgccag cacctacatc gttgccgccg cgcgcaatgt tcatgcgctg
     2941 gtgccggtca tgctcggcac catggctgcg gaccaggaac gtccgaccgg cgcgtggcag
     3001 gccgaatggc tggcactgcc acaggccttc gtgctcaccc acggcgcact cgaccacgcc
     3061 ggcagcatcg cccgaggcat ggtggccgcc ccagccagca tgcagcgcaa tctcgatctc
     3121 acccacggac tgatcgttgc cgaagcggtg atgatgggcc tcgcgccaca acttgggcgt
     3181 gccgaggcgc atcacgcggt caagcacgcc tgcgatgtcg cgttgaccga ggacatttcg
     3241 ctcgccgacg ccctcgcccg cgaaccagcg ctcgccggca aactcaaccg cgccgagatc
     3301 gagcgcctca ccgacccggc aaactatctg ggcagcaccg acgcgttcat cgatcgcgtc
     3361 ctggcgctcg ccacctgacg cagcgttcga cagtttcgca cgaaactgac gttacggcgc
     3421 cattgcttat cgcccggcca cccgctagcg tgcccggcca ataatgacac cggatcgtcg
     3481 gcccggcagc ggccggcgcg gacgtcgagg ggcggaaaat accgccacgg acaggggtct
     3541 ggctttttat ggccggcggg ctaaagccac gcggcatggg aggtacgtgg atggcgagga
     3601 ttcgtctcgt cacgcatggt tgtcggttgt tctcggaacc cgggtcctgt gatccggagc
     3661 gtccggcccg gcgcgggctg gacgtcaacc cgcattccga tctaaccctc cctggtggac
     3721 aagaacgtgg acggctcatc ggccgacgcc acatacgctg actttcatcg caaccacaaa
     3781 gggaacgaca agaccatggc tgaagtttcg tgccgcaagg tagtcaagaa ctacgaaggc
     3841 ggcgtgcaag cggtgaaagg catcgacctt gaaatcgctg accaggaatt cgttgtcctg
     3901 gtcggcccgt caggctgcgg caaatccacg acgctccgta tgatcgccgg cctggaagag
     3961 atttccgacg gcgagatctg gatcggcggc gacgttgtga acgacgtacc gccgcgcgac
     4021 cgcgacatcg ccatggtgtt ccagaactac gccctgtatc cacacatgag tgtgttcgac
     4081 aacatggcgt tcggcctgaa gctccgcaag ttccccaagg acgagatcaa gcggcgggtg
     4141 gacgaggcgg cgcgcatcct cgatatcggc atgctgctcg accgcaagcc ccgcgcgttg
     4201 tcgggcggcc agcgccagcg cgtggcaatg ggtcgcgcga tcgtccgcaa ccccaaggtg
     4261 ttcctgttcg acgagccgct gtccaacctg gacgccaagc tgcgcgtgca gatgcgcacc
     4321 gagatcaaga aagtgcacca gacggtgcgt accaccacgg tctacgtgac gcacgaccag
     4381 gtggaggcaa tgaccttggc cgaccgcgtt gtggtgatga accacggcgt gatcgagcag
     4441 gtcggtgcgc cgcaggaatt ataccacaac ccgacgacac ggttcgtggc ggggttcatc
     4501 ggctcgccgg cgatgaactt ccttccggtc cgggtggagg atggcacggg cggcggcctg
     4561 gcggtgcgcg tcaccgacca gatcaagctt cccattcccc ctgcccgcaa cgaccgctac
     4621 ggcaagtaca aggaccgcga aatggttctc gggctgcggc cggagcacct gttcgaatac
     4681 cacgagaacg ggcgccctgg atgggagcgc gtcgagctgc cggtcgatgt ggtcgagccg
     4741 atgggcatgg agacgatggt gcacttcttc atcggccacg agccggtatg cgcacgcgtc
     4801 gatcccgcga cgcacgccga acccggcgag atcctgccgc tgacagcgga catggggcag
     4861 atgcacctga tggagccgga caccggccgc gtggtctgac aggcggcagc ccctccccta
     4921 actgaagcgg gcgggagaga tcgtcgcgac gatctctccc gcctcgccag ggtcccgcgc
     4981 caggatcaaa tcggcacaga gacgcccggc ggcgggtgaa gtctggatgc cgtaaccgcc
     5041 ctgccccacg caccagaaga agccagccgc gtccggcgcc cagccgaacg ccaggctgcc
     5101 gtcgggagtg aatgtgcgca ggcccgccca gcttcgctcg acgcgtcgca cgtcaatgtc
     5161 cagcgcctgc tgcatgcggt cgattgcgac agcgatgtcg aactcgtccg gctgcacatc
     5221 gtgaggatac atcggggtct tgtcagccgg cgagaccata agccgggtgc gcgcttcagg
     5281 ccgggcatac caggtttccc cgatatcgca gaccatcggc cagtgctcca cctgccaggg
     5341 cgccggatcg atgatcacgc cggttcggcg gcacggtttc agcccaagtg gcgcgacccc
     5401 ggcgatcgcc gccacctcgt cgccccagga gcccgaggca ttcaccacgg tccgagcacg
     5461 gaacacgtcg ccactgctgg tctccacatc ccaggcgcca tcgcgccgct cgatccgtcc
     5521 cgcgcggttg cgcagcgcga gcacgccgct atgggcacgc aattgccgca gatagccctg
     5581 atgcaaagct gcaacgtcga tatcgaacgc atcctcctcg atcgccgcaa gcaccgcata
     5641 gtcaggccgc atggccggga tcatcgcgcg caggtcatcg agctgcagcg ggcgaatgcc
     5701 acctcccagc accgcttcaa gctccgcgcg ctggttctcg ggcgccacca gcacctccga
     5761 ccgttgcgac aataacggtg tttcagtgaa gcccgccggt ggtgacacga ggaacgagcg
     5821 cgacaatccg gtcagatgct gcacatcgtg cggcccatag gtcaggatcc acatcgccgc
     5881 ggagcggccg gttgtgtggt agccggccgc cgcctctgcc tcgatcagcg ccacccgaca
     5941 gtccggtgaa agtgcggcag ccgccgtcga gccggcgatg ccggcgccga tcacgaccgc
     6001 atcgaattga taggtatcca taggttctcc gaccgacaca cactcgtccc cagcctgact
     6061 ggtcttcccc tggtgttgac accagcttgc agcgcacgac tgcccgagct ttcctgcgag
     6121 ggccggcatt taattcaatc acaaagttcg cgaaggggca cgaagatcac gaaggacatt
     6181 tcagccgccc ttcgtcccct tcataaaccg aaggtccaga gcaagcctct tggcggcctt
     6241 cgcgttcctt cgcgaacttt gtcttgaata ctatagaccg cacgcaggat caggcattca
     6301 agacgagggc tccgttgttg gacaagacga cgcctcgtag attggacaca tggcgctcaa
     6361 acggatggac aacgtaggga tcgtcgtaga acacctcgaa gagacgattg atttctttcg
     6421 cgagctcggc cttgagctcg aagggcgggc cacgatcgaa gaagaatggg ctggacgtgt
     6481 caccggactg ggcgatcagc gcgtcgagat tgccatgatg cgcacgccgg acggtcacgg
     6541 ccggctcgag ctctcccgct tcctcacgcc gcctgccatt gcggatcacc gcaacgcccc
     6601 ggtgaacgct cttggctacc tccgcgtcat gttcgccgtg gacgacatcg atgagacgct
     6661 tgaaaggctc cgcaggcgcg gcacgcagct cgtaggccag gtagtgcagt ataaagacgc
     6721 gtatcggctc tgttacatcc gcgggcctga aggtctactc atcggactcg cccaagaact
     6781 cagctgagca gattgagcgg accgttgccg acatgcacgt cccgcccctc cagcctgcca
     6841 tcataaacgc agacgtgcca cactggaatc aatcgagttc cggagtcgca caatgcagtc
     6901 ccgcatcacc ggtaccacca tgcccgtgtt ggaaatcggg ctggagccag gcgacgtatt
     6961 ggtggcgccg cccggcgaac tctcatggat gaccgccaac gttcagatgc gcaccaccac
     7021 cgcgaccgcc ggcgcgcgcg gcctgtgggg tgcgatcacc cgcgccgtct ccggcggcgg
     7081 cctcttcatg accgaattcg ccactgcggg cggtcagggt gcggtggcgt tcgccgccaa
     7141 ggtgccggga cagatcatgc caatcgacgt ccagcccggg cgcggttatc tggttcaccg
     7201 tcacggcttc ctgtgcggca tgcagggcat cgagctgggc atggccctgc aacgctcgct
     7261 cggcggcggc atcttcggcg gcgagggctt cgtcctgcaa cgcctgaccg gcaacggaca
     7321 ggcctgggtg gaactgggcg gcgagattgt cacccatgag ctcggcgccg gcgagacgct
     7381 gctggtacat ccgggccatg tcgggatgtt cgaggaaagc gtctcgttcg acatcaccat
     7441 gatccgcggc atcaccaatg cgatcttcgg tggcgacggg ctgttcctgg tgcaactgac
     7501 tggccccggc cgcatctggc tgcagacgct gaccatgcca aacctggcgc atgcgctgat
     7561 gccctacctc ggtcggcagg aaccggccag caccgcacag accgttgaag cgggagccgc
     7621 aggagccgta ctgcgggata tcttcggcgg gcaaaggtaa caccagccct cgtcgagcgg
     7681 gaccgatcag ccgttctcgt catgccgggc gaaggcccgg aatccacgcc tttcgagccc
     7741 tcttcacgcc acaccggtac aagcccgcgg caacccgccc cccgtcacgc cgctcctctc
     7801 cgccccgcca ccatcgtctg cgcctgctgc cacaccagcg ccttcacccg caccagccgc
     7861 tccccctcga agtccggcca atgcagcgcc cgcagcgaca cctcgcgggc gcgctggaac
     7921 accatcagcg gccccagaat ggccagtgtc agcagcaccg tctcctccgc gtctgcaggc
     7981 tgatcgatca gcctgccgac aatcgatgcg cacgcgccca gcagcggcgc catgacgcgc
     8041 cggtgcagga tctcatacgc ctccgtcggc gcgatctgct cgcgcaggaa gaacgccacc
     8101 aacgcgggtg gatcccgagg gcccaccaga cggtcagcga acgcgtccag cagccggtgc
     8161 agcaactcca gcagcgcctc acgcgacgca ccaggctgtt ccatcatcgc caggaaaggc
     8221 gccacccgcg cctcacccat cccggccagg tggtcggcac aggcacggta aagctcgcgc
     8281 ttgccgccga aatagtaatg caaggcggcc aggttcacgc cggcccggcg ggcgatcagc
     8341 cgggtggacg cgtcctcgaa gccatgggtt ccgaacacct ccaccgctgt ctcgatgatg
     8401 tgcagccgcg tcgcgtcacc gcgcgcgtaa ccttgaaccg gccgtttgcc gccacgatct
     8461 ttggccactt gactgatatt ggtcatccga ctgatattcg cgggacctta gtgcgtgagg
     8521 gacctatgcg caacatgcgc gccctgatgt cgctcgtcgc actgctggcg ggctgcacgg
     8581 tcggcccgga ttaccatcct ccggacactc cgatgccggc gcggttcagc ggccccgcgt
     8641 ctgccgcgac cgcggacatc gcacaatggt ggcattcgtt cggcgatccg gaactcgatc
     8701 acctgatcga gcaggcaatg cgcgacaatc ccgatgtaca gaccgccgcg tcgcgggtcc
     8761 gcgaggcgcg gctgcaggaa atcgtcgccg gcgccccgct ctggccgagc ctgcgggcgg
     8821 atgaccaggc agcgcatacc catctcagcg gcaatgcgat ctcgcttggc aatctcggca
     8881 cgctgacagg aggtgccata ccgagcggtg gatcgagcag cctgtcgtcg cttggcctgt
     8941 ccggcaccag cttcaacacc taccagctcg gcttcgacgc ttcgtgggaa ctggatctgt
     9001 tcggcaagac gcgccgttcc gtcgaggcgt cgcgtgattc caccggcgcg gcggtctggg
     9061 atcggcggga tacccaggtg tcactcgctg ccgaggttgc caacacatgg ttcgcgctgc
     9121 acgccgccca ggcgcgcctc gacgtcgcga agggcgagct ggcccggcaa caggctctga
     9181 tggccgttct acggacgcgt cagagcaccg gtttcgtcac cggcctggac gtcgcacagc
     9241 aggacacgca ggtcaccgcc gccgctgccg ccgtgccccc atttcaggct gaaatcgacg
     9301 cgcggcgcca cgcgcttgcc gtgctggttg gagaagcgcc cgaaacgctg gaccttcctg
     9361 ccaccggcgc cggtagcgcg acgccccctg gcattccgcc cggcctgccc tccgatctgc
     9421 tgcgccggcg acccgacatc cgctcggccg agcgccagct tgcggcggcg acagcgaata
     9481 tcggcgtcgc ggtcgctgac tattacccgc agatcaccct caccgcatcg ccgtcgctga
     9541 tcagcacggc gctgtcgaac ctgctcacct ggggcagccg caatctgagc ctgagcgccg
     9601 gtcttgtttg gccgctgttc aacgccggca ccacgaaggc caaggtcggc atcgccaatg
     9661 aacaccagca gcaggcgttg ctggcatatc gcaagaccgt gctgacggcg ctgaaggatg
     9721 tggaagacgc gctgtcgcag tatcagtcgg acagggacag acaggcggcg ctgcaacgtt
     9781 cactgaatgc ggcgggcaac gcggccgctg tcgcgcacgg ccaatatcgc gccggactga
     9841 tcccgttctc gaccgtcttg accaccgagg ctgcgctcta ttccgcgcgg gaccagagcg
     9901 cgcaggccaa tgctgcgctg aaccaggatc tggtggcgtt gtacaaggcg cttggcggcg
     9961 gctggagcga ggaaaccacc gaggccgcac gatgaatcgc gtgcgccaga tcctgatgat
    10021 cctgatcgcc gtgggcgtcg gcggctggct ggcctggcgc gcgtggggac cgcatcccga
    10081 agaccgcgtc gcgttgtccg gctacatcga gggcgagtac ctgtatctct ccgccccgat
    10141 ctccgggttc gtcgcgcggg tcgatgtggc gcgcggcgat cgcgtcgccg ccggacaaaa
    10201 gctcttcgcc atggacgatg cgccgttgcg cgcgcagcgc gaccaggcgg cggcgaacgt
    10261 cacgcaggcc caggcgcaga tcggatcagc gcaggccaag ctggcgcagt cacgcgcgac
    10321 cgccgaagca acacaggccc aggcaaccaa cgctgccgaa gacctgaagc gctatcgcag
    10381 tgcgcttcgc gtcaacgccg aaaccgtatc gcgccagcag gtggatacag ccgtagctac
    10441 cgccgccaac tccgccggcc aggctgacgc ggcacgcagc gacgtcgcgg cacaagaggc
    10501 acagatcgcg gcagcaaagg cccaggctgc ggcgcaacag gccgcgttgg cggatgtggc
    10561 ggcccggttg gcgcagctcg agccgcacgc gccggcggcc gcgcgggcgc aggacgtatt
    10621 ttaccagaaa ggtgaatggg tggcggagaa ccagcccgtc atcagcctgc tgcctgacga
    10681 caaggtgaag gtgcgcttct tcgtccctga agcgcaggtc gcccgatacc ggccaggcca
    10741 gacggtgcat ttcgattgcg acggctgcgc cacaggtctc acagcgcgga tcaactatgt
    10801 cagcacgcaa cccgaatata cgccgccgat catctacagc cgcagtacgc gcgacaaatt
    10861 ggtattcctg gtcgaagcgc tgcctgccga tggccgcaac ctgacgccag gcttgccagt
    10921 cgatgtggag cctctgcgat gatgcaattc gattgtgccc acctcgctcc cctgtcattc
    10981 cgaggagcga agcgacgaag caatctcgga cgaattgcac gcgcgcaacc cgtccaggat
    11041 cgcttcgtcg tcactgcgct cctcaccacg acatgcagcg acccacagga acccgtctga
    11101 tggtcctcgc catcgacgtc cacgatctga acaagagttt tggcgcgcat caggtggtgc
    11161 gtgacgtggc gatccaggtc gaagccggtc acatctgcgg cttcctcggc ccgaacggct
    11221 ccggcaagac gaccaccctt cgtatgctgt gcggattgct gacccccgac accggcgcgg
    11281 gaacatgtct cggcttcgac atcctgaagc agaccgacca gatcaagcgc aacaccggct
    11341 acatgacgca gcgcttctcg ctctacgaag acctgaccgt cgaagagaac ctggatttca
    11401 tcgcccgcat gtatggtttg gaccggcggc gcgaacggct gcagcaagca ctgggccggc
    11461 tcggcctggc ggataggcgc gaccagcgcg ccggcacgct ctcgggcgga tggaaacaac
    11521 gcctggcgct cgccgcctgc gtacttcacc agccgcgatt gctgttgctc gacgagccaa
    11581 ccgccggcgt cgatccgaaa gcccggcgcg aattctggga cgaactgcat cgcctcgccg
    11641 gcgaaggcat aaccgtactc gtgtccaccc actacatgga cgaggcggaa cgctgccacg
    11701 acgtggccta catcgtcggc ggccgcctga taggccgcgg caccgccgaa gaactcgtcg
    11761 aacaggccgg gctgatcacc ttcctcgccg aaggcgcgcg cgcggatcgg ctggccgagg
    11821 aactgaaggg ccaccctggc gtggtgatgg cgacgccgtt cggccaggac ctgcatgtca
    11881 gcggcactga tcgcgcggcg ctggaaaatg cgatcgcgcc ccaccgccac gaaccctacc
    11941 gttggacgga aacccgcccg acactggaag aagtcttcat ctacatgatg gatgcggacg
    12001 catgatccgc ttctcattcg gccgtgtgct ggcggtgctg gtaaaggaat tccgccagat
    12061 gatgcgcggc cagccgagcg tgatcctgtc gatcccgatc atgcagctgc tgctgttcgg
    12121 ctacgcgatc aacgataacc cgcaccattt gccgaccggc gtgtacatcc aggatcaggg
    12181 cgagttcgcg cgttccatcc tcgcggcgat gcgcaacacc ggatacttcg acatcgtcgc
    12241 gaccgcgcga aactccgccg agctcgatcg gctgatggcg ctgggcaaga tcacgttcag
    12301 cgtcacgatc cccggcgact tcacccgccg cgtgatccgc cacgacaacg cgcagatcct
    12361 cgtggaagcc gacgcatcgg atccgactgc caccggcagt gcgatcgccg cactggcggc
    12421 cttgccaacc acggcactcg cgcgcgacct gatcggcgcc gtcacacccg cggcaaccag
    12481 cgcgccattc accgccgtgg tgcacagccg ctacaatccc gaagcgatca cgtcgtacaa
    12541 cattgtgccc ggactgctcg gcaccgtgtt gtccatgaca ctggtcttca tgaccgcgca
    12601 aagcctcgcc aaggaacgcg aggtcggcac gttggagggc ctgctcgcca cgccggtccg
    12661 ccctgtcgaa gtgctcattg gcaagctcgc tccctatgtc ggcatcggca tcctgcaaac
    12721 cggcatcatc ctgctgctgg cgcgtgcatt gttcgatgtg cccgaggcag gcggatggga
    12781 cgcgcttgcc atcggcgtgg tgctgttcat cgtcggcagt ctggcacttg gcttcctggt
    12841 ctccaccttt gcgcgcactc aacaggaagc gcagctcatc gcgcaattct atttcatgcc
    12901 gtctttcctg ttgtcgggct tcttctttcc gttcgccggc atgccgggct gggcgcaggc
    12961 gatcggctcg ctggtgccgg tgacgcattt cctgcgcatc gttcgcggct cgctgctcaa
    13021 ggggcagggc attgccgatg cgtggccaag ccttgccgcg cttatgctgt tcgtcctggg
    13081 cgtgagcgtg ctggcgatcc tgcgctaccg cacaacgctg gattaatttg ttggcgcctc
    13141 gcgcaacaga tcgttacaat ccgtcaatcg caccgaactc gaggggaaac accatggact
    13201 atccctatga cgtcggcccg tattcccgca cagtgaccac cacatcgccg gacgcccagc
    13261 gatggttcga tcgcggcctc aactggtgct tcggcttcca tcacgaggaa gccatccgct
    13321 gcttcgaaaa ggccctcgag gccgatgcga actgtgccat ggcgcattgg ggaatcggct
    13381 atgccgctgg tcccaactac aattttccct gggagctgat ggaccccgcc ggcaaggctg
    13441 caacgctcgg ccgcgcctat gacgccacgc gcgcggcgct cgcgctcgcc ccaaatgtca
    13501 gcgcccccga acgcgcgctg atcgaagcac tgccctcccg ctatccgcag cgcgatccca
    13561 tcgaggatca acgtccctgg aataatgcgt tcgccgacgc gatgcgtctc gttcatcagg
    13621 cgcatccaga cgacctcgac atacgcaacg tcttcgtcga ggcgatcatg aaccgaacgc
    13681 cttggcgcat gtgggatctg cgcaccggcg cgaccgcacc ggatgccgga acgaccgaag
    13741 cgcgcgaagt gctcgagacc gccttcagca cgctgccggg tgcgatgagc caccccggcc
    13801 tgctgcatct gcacgtccat ctgatggaga tgtcgccgaa ccccgaggcg gcattggtca
    13861 ccggcgatcg gctgcgggag atcagcccgg acatgggcca tctcactcac atgcctacgc
    13921 acatagatat acagtgtggc cactatcgtg acgcgatgct gtggaaccag agggccatca
    13981 ttgccgaccg caagttctac gaccgcgtcg ggccgatgaa cttctactct gggtaccgta
    14041 tccacaacta tcacttcgcg gcctacggcg cgatgttcct tggccagtac gagcccgcca
    14101 ttgccgccgc caatgaactg gtcgagacga tgccggagga cttcctgcgc attccctcgc
    14161 cgccgatggc gaacttcttc gagagctacg tcgccgttcg gctgcacgtg atgatccgct
    14221 tcggcaaatg gcacgagatc atcgcgcaga cgctgccggc cgaccccgag ctgtactgca
    14281 acacggtggc aatgacgcac tatgcgaagg gcgtcgcgca tgccgccctc ggcgacgttc
    14341 cggcggccga ggccgagcag gcactgttcc gtgacgccgc gaagcgtgtc ccggacacgc
    14401 gctacatcca caacgtgaaa tgcgtgtcgc tgctggccat cgccgagcag atgctggcag
    14461 gtgagatcgc ctatcgccgc ggcgagcacg atgcggcatt cgcccatctg cgtgccgcaa
    14521 tctcgttgga agacgatctg ccctacgacg aaccgtgggg ctggatgcag cccgtccgcc
    14581 acgccctggg tgcgctgcta ttggagcagg gacgcgccgc ggaagccgaa gtggtgtatc
    14641 gcgaggatct cggtctcggc ggcacattgc cgcgggcaca gatccacccg gacaatgtgt
    14701 gggcactgcg tggcctgctc gactgcctgg aacgtcgtgg cgagagagcc gaggcgaccc
    14761 tgatacgcca acgggtgacc tttgctgagg cgcgcgctga tgtgccggtg gcggtgtcgt
    14821 gtttctgcgc gcgagggtaa ggcgtccacc tttcagttcc acctgggctg cgcgacaatc
    14881 agcagcagga agaacagcgc gatcaatgta gccggaagcg ccagcagaag aagcacgccg
    14941 ttggccagcc gcaagtggcc gtttgcgcgc agccacactc ctccgcccgg cacgccgccg
    15001 acgccaccga gcattgccag ccacagcagg atgttgaacg acgagactgt accatcgctc
    15061 aatccccaca ggaaaaagaa caccaccact gccgccgcca gggcatcgat gccaaacaga
    15121 atccggaacg ctatcatacc gagcaggcta ctgtaccgcc ggccgctggc aagcaccgac
    15181 aaagcttgcc tgatccgccc agacgcggaa ccatgcgtac ggacgtaggg aggaagcggc
    15241 gatggtcgat ctggttgtgc gtggcgatac cgtggtgaca ccgcagggcg tcggtgccta
    15301 cgacatcgcg atcgccggcg agcgtatcgt cgccatggca gccagcggca ctttccccat
    15361 ccccgacggc gcgcgcctga tcgatgccac cggcaagatc gtcatgcccg gcggcatcga
    15421 tccgcacgtc cactgcaagt ggttcatgcc ccatccggac ggcaccgcgg cagacaccga
    15481 tccgcccgat gtggtgggac gcgccgcact gcacggcggc accacgacaa tgatcgactt
    15541 cacccgcgcc aaccaggacc ccagtctcca gaaggccatc gaaaagcgcg agcaggactg
    15601 gaagggccac tccccctgcg actatgccca gcacatcatg gtcgaaggcg cgctgccgat
    15661 cgacctgccc gggcagctcg ccgaggcgat ccaggcaggc ttcgccacag tgaagatctt
    15721 caccaccgac atcacgccgt cgcgcaaggg acgcatggtg gatttcggtg acatctggga
    15781 agtgttccag gtgctgaagg caagcggtgg cctcggggtg atccattccg aggacaacga
    15841 catcgtgatg cacatgtacg gcaagctgat tcgcgagggc cgcaccggct tcaccaatat
    15901 ggccgaggtc cacaacaccc tgtccgagga tatttccttc cgccgcgtga tccgcctggc
    15961 cgaaaaggtc ggcgcgacgc tgtacatgat gcacacctcc gcggcgagcg gtgtgtccgc
    16021 gatccgcgaa gcccgggcac gcggcgtgtc gatctatggc gagtcactac atcagtatat
    16081 gctgtacacg aacgaggact ataagcgcca gaacgggcag atctatcaca catatccctc
    16141 gctgaagtcg gccgaggatc aggcggccct gtggagcggc actctggatg gctccatcaa
    16201 ctgcgtcgcc accgacgaga tctgctgcac gctggcaaag aagctgcaag gctcgcgcat
    16261 cgatgacacg acgggcggca atgccggcgt cgagcctcgg gtatcgctga tgtataccga
    16321 aatggtgcag aaccggcgtt actcgctgag ccggttcgtc gatctggtct ccacgaacgc
    16381 agcgaaaatc atggggctgt atccgcgcaa gggtgcgctg gccgcggggt cggatgcgga
    16441 tatctgcgtg ctcgatccgg gcgacaatcg ggtgatccgc gctcaggatc tgcacgaggc
    16501 agactattcg ccatgggaag gccgcaagat ggcggcctgg ccctgcctga cggtgctccg
    16561 cggcaaggtg gtggtggaga atggcgcgtg gaagggtgca gtgaccgacg gcaaatggca
    16621 gcaccggaaa gttgcctcgg aaatgttggc agggcccatg ctgtgacaaa ggaattagga
    16681 accacagatg gacacggatc atctgtctgt atctgtgtgt atctgtgccc atctgtcgtt
    16741 ccatatagct gactatgact gacgctgaac tgcgggcgtt gttgctggat tgcctgtcgc
    16801 tgtggggcgt gaagggacgc gtcgaagcag ctgatcccgg catgcggatc attgccggca
    16861 ccaccacgct tgcggtcgaa cgtgcgcccg ccgatatgcg gcccgtccgc tggctgctgc
    16921 atcgtcccaa tcgcgcacca cgtgtcgcgc cgtcgattgg cgccctgctg acagcgctgc
    16981 gcaatgcgct cggtgcagag gccgggaaca aactgagggt tggaacatga aacaggacga
    17041 acgcggcctg ccgctctcga ccgacagcac cgaggcggtc cacagcttcg atcgcgcagt
    17101 cgagcatttc ctgaagttcc acgccgacac gatggcactg gtgggccgtg ccctcgccgc
    17161 ggatcccggc ttcgtcatgg gacactgcct caaggcctat ctgctgctga tagcggccaa
    17221 tcctgccaac cgcgcacaga tcgacgcgac gctggcaagc gcacaagccg gtgccgccaa
    17281 catcaccacg cgcgagagga tgcatctcgc cgccgcggaa cactggcatc gtggcgcgct
    17341 cgacgaatcc ttcaggatct ggggcgagat actcgacgcc catcccaccg acctgctgac
    17401 gttccgtatc tccgacacga tctggttccg ccatggccag acccagccga tcctgaccca
    17461 ggcggatcgc gtcacaccgc gctggtcggc ggatctgccg ggctatgatt gcgcccaaac
    17521 catctgggcg ttcgcgcatg aagaagtcgg tgacaccaaa aacgccgaac gtgcgatcga
    17581 cgccgcattg gaacgcgacg cgaccaatta cttcgcgcac cacgtcaagg cgcacatcat
    17641 ggataccgac tgccgcgcgc gcgagggcag cgactggctg ggcagccagg tgcccaactg
    17701 gtcgctgggc aacaacctga tccatcatct ctggtggcac cgcgccctgc tgcggctcga
    17761 cctcggcgaa gtggacgccg tgatggcaag ctacgatcag gacatccgca acttcgacgc
    17821 cccgatgacg aaggccaccc ccgaccacta cgtcgacctc cagaacgccg cggctctgtt
    17881 gtggcgcctg gaacaggccg gcctggatgt cgggaaccgg tgggaggaat tggcggacaa
    17941 agccgaagcc cgcatcggcg aggccggcca tctgctgatg gtgccgcacc tgatgatggc
//
LOCUS       DATGEM010000248         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_943162, whole genome shotgun sequence.
ACCESSION   DATGEM010000248 DATGEM010000000
VERSION     DATGEM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943162"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /gene="nuoG"
                     /locus_tag="VKQ27_07885"
     CDS             <1..1904
                     /gene="nuoG"
                     /locus_tag="VKQ27_07885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HLY88888.1"
                     /translation="AGNCRMCLVEIEKAPPKPISSCTYPVADGMVVHTDSPMVRNGRR
                     GVMEFLLINHPLDCPICDQGGECDLQDQAMGYGMDHSRYAENKRAVKDKNLGPLVKTV
                     MTRCIHCTRCIRFISEVAGVPDLGATSRGEHMEVGTYVEKALSSELSANIIDLCPVGA
                     LTSKPYAFVARPWELSKVDSVDVLDAVGCNIRIDARGPEVLRILPRLNEDVNEEWLGD
                     KSRFALDGLRRRRLDRPWVRRGGKLVEATWAEAFSAIAEKLHGKSGDRIGAVAGDLCD
                     AESMLALKDLMGALGSANLDCRQDGAHLNATRRDFYCFNSSINGIDDADAILLVGTNP
                     RREAPVLNARIRKRWLHASIPIGLIGSEVDLTYRVQQLGTNPTALNAEFFRDAKKPMI
                     IVGQAALARPDGAAVLAAAWRLAANVGALTPEWHGFNVLHTAAARVGALDLGFVPVAN
                     GKSLAQMLNGGVDVLWLLGADEFDTSQIGANTFVVYQGHHGDAGAARADVILPGAAYT
                     EKPGTYVNTEGRVQRGLRSVYPPGDAREDWTILRAFSDVVGHRLPYDSIDALRKRMEQ
                     VNPVFGRIGFLPRFGATDQTGPAGDPTVLSDVPFEPAVLNYYQTDPISRASATMAACT
                     STQLAEAAE"
     gene            1909..2946
                     /gene="nuoH"
                     /locus_tag="VKQ27_07890"
     CDS             1909..2946
                     /gene="nuoH"
                     /locus_tag="VKQ27_07890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HLY88889.1"
                     /translation="MEAFFLHTIIGRLILTVLEALLLLVPLLIGVAYLTYAERKVLAA
                     MQLRKGPNVVGPFGLWQPFADAIKMIMKETVIPAGANRLLFLLAPMLTMGLALIAWAV
                     IPVADGWVNADINVGILYLFAISSLGVYGIIIAGWASNSKYAFLGAMRSAAQMVSYEV
                     SMGFVMVSVLLCVGSLNLTDIVQGQNHYWFGFIPAWYAIPLFPMFIVFFISALAETNR
                     SPFDLPEGESEIVAGFFVEYSAMAFALFFLGEYANMILMSGMTTILFLGGWLPLFPVA
                     PFIWIPGVIWFVLKVCFCLFVFLWVRATFPRYRYDQLMRLGWKVFLPLSLLWLVITGG
                     ALMLFGWLPHA"
     gene            2959..3447
                     /gene="nuoI"
                     /locus_tag="VKQ27_07895"
     CDS             2959..3447
                     /gene="nuoI"
                     /locus_tag="VKQ27_07895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HLY88890.1"
                     /translation="MAFLDRTARSLLLGELVAGLGLTLRYFFKPKVTINYPYEKGPLS
                     PRFKGEHALRRYPNGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKC
                     IYCGLCEEACPVDAIVEGPNFEFAAETREELLYNKEKLLDNGDRWEAEIARRLELDAP
                     YR"
     gene            3444..4127
                     /locus_tag="VKQ27_07900"
     CDS             3444..4127
                     /locus_tag="VKQ27_07900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HLY88891.1"
                     /translation="MIATVFFYIFAVVLLISAAMVVSSRNPVHSVLFLILSFFNAAAL
                     FLIAGAEFLAMILMIVYVGAVAVLFLFVVMMLDIDFAELRGGFQRYLPIGATVGAVLF
                     IELALVISGWKFAPGAPTLRLAPVPAQVSNTAALGRLLYTDYVFLFQAAGLLLLVAMI
                     GAIVLTLRDRKTSRHQIIRVQTERTVAETLEMVTVSIGAGVKDIGIFEPKVAEQEEPE
                     SAEETHHGH"
     gene            4117..4431
                     /gene="nuoK"
                     /locus_tag="VKQ27_07905"
     CDS             4117..4431
                     /gene="nuoK"
                     /locus_tag="VKQ27_07905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HLY88892.1"
                     /translation="MGTEVGVGHYLVVSAILLVLGIFGIFLNRKNVIIILMSIELILL
                     AVNLNLVAFSASLGDLVGQVFAMFVLTVAAAEAAIGLAIVVIYFRNRGSIQVEDVNLM
                     KG"
     gene            4433..6364
                     /gene="nuoL"
                     /locus_tag="VKQ27_07910"
     CDS             4433..6364
                     /gene="nuoL"
                     /locus_tag="VKQ27_07910"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HLY88893.1"
                     /translation="MIYAVAVFAPLFGALVAGLLGRSIGDRAAMAVSVLCMLVAAALG
                     PIACFQLVGGDASAGTVSLGTWVDAGHFHASWALRYDTLSAVMVAMVCFVAMLIHVYS
                     IGYMAHDAYPRYRFFSYLSLFTFSMLMLVTADNLLQLFFGWEGVGLCSYLLIGYWYDR
                     PSANAAAIKAFITNRIGDLFFAVGIALIFFKFGSIEFSTIFPAVPQHGADTYSVLGGT
                     YPAFEVIGVLLFIGAMGKSAQIGLHVWLPDAMEGPTPVSALIHAATMVTAGVFLVARM
                     SPIYEFAPFALGLVTFIGATTALFAATIGCVQNDIKRVIAYSTCSQLGYMFAAAGVGA
                     FQVSIFHLITHAFFKALLFLSAGSVIHAMSDEQDMRKMGGLWRMIPWTCAMMWIGSLA
                     LAGIPPFAGYYSKDAIISATYASGTGIGMYGFICTVLAALLTAFYSWRLLFMTFNGKS
                     RADHHVLEHVHESPWVMIGPLLVLGTGAIVAGKLLDGTFIGEGWQEFWNGAIFNAPNN
                     HVLAGLEHVPGWVEMSPLVVGLLGIALAYVMYIANPLLPMRLATMFRPIYLFLLNKWY
                     FDELYDAIFVQPAIRLARMLWQTGDATIIDGVPNGLAEITVDGSRQAVKIQTGSLAVY
                     AFVMLIGVVVLVGLFLVSR"
     gene            6361..>6790
                     /locus_tag="VKQ27_07915"
     CDS             6361..>6790
                     /locus_tag="VKQ27_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLY88894.1"
                     /translation="MNAAGFPILSLIIWLPLVGGVIIMMVRGDEETVASNARWTALWT
                     SLIVLVLSLVLWVKFDTGEAGFQFIEEIPWLPEYGVGYKLGVDGISVLFVLLSTLLTP
                     ICILASWESIKRSVREYMLTFLILETMMVGMFCALDFVVFY"
BASE COUNT         1068 a         1944 c         2293 g         1485 t
ORIGIN      
        1 tcgccggcaa ctgccgtatg tgcctggtgg aaatcgagaa agcgcccccg aagcccatta
       61 gttcctgcac ctatccggtg gcggacggca tggtggtgca caccgattcg cccatggtgc
      121 gcaacgggcg gcgcggcgtg atggagttcc tgctgatcaa ccatccgctc gactgcccga
      181 tctgcgatca gggcggcgag tgcgatctgc aggaccaggc catgggctat ggcatggatc
      241 actcgcgcta tgcagagaac aagcgcgcgg tgaaggacaa gaaccttggt ccgctggtga
      301 aaacggtgat gacgcgctgc atccactgca cgcgatgcat ccgtttcatc agcgaggtgg
      361 ccggtgtgcc ggaccttggt gcgacgagcc gcggcgagca tatggaagtc ggcacctatg
      421 tggagaaggc gctgtcgtcc gagttgtccg ccaacatcat tgatctgtgc ccggttggcg
      481 cgctgacctc gaagccgtat gcgtttgtgg cgcggccgtg ggagctgtcg aaggtcgata
      541 gcgtcgatgt gctggatgcg gttggctgca acatccgcat cgatgcgcgc gggccggaag
      601 tgctgcgcat tctgcctcga ctgaatgaag acgtgaacga ggagtggctg ggcgacaagt
      661 cgcgcttcgc tctcgatggg ctgcgccggc ggcggctgga taggccgtgg gtgcggcgtg
      721 gtggcaagct ggtcgaggcg acgtgggcgg aggccttctc cgcgatcgcg gagaagctgc
      781 acggcaagag cggtgatcgt atcggcgcgg tggcgggcga tctgtgcgat gccgagagta
      841 tgctggcgtt gaaagacctg atgggcgcgc tcggttcggc caatctcgat tgccggcagg
      901 atggcgcgca tctcaacgcc acgcgtcgcg acttctactg cttcaacagc tcgatcaacg
      961 gcatcgacga cgcggatgcg atcctgctgg ttggcacgaa tccacgccgc gaagcgccag
     1021 tgctgaacgc gcgcatccgc aagcgttggc tgcatgcctc gatcccgatc ggactgatcg
     1081 gtagcgaggt agacctgacg tatcgcgtgc agcagcttgg caccaacccg acggcattga
     1141 acgctgagtt cttccgcgat gccaagaagc cgatgatcat cgtgggccag gcggcgctcg
     1201 cgcggccgga tggcgcagcg gtgctggcgg cggcgtggcg tctcgcggcg aatgttggcg
     1261 cactgacgcc ggaatggcat ggcttcaacg tgctgcacac cgccgcggcg cgtgttggcg
     1321 cgctggacct tggcttcgtg ccggtcgcga acggtaagtc gttggcgcag atgctgaatg
     1381 gcggcgtcga tgtgctgtgg ctgctcgggg cggacgagtt cgacacgtcg caaatcggtg
     1441 ccaatacgtt tgtggtgtat caggggcatc acggcgatgc cggcgcggcg cgggcagatg
     1501 tgatcctgcc gggcgcggct tatacagaaa agcccggcac ttatgtcaat accgaaggcc
     1561 gtgtgcagcg ggggctgcgt tccgtctacc caccgggcga tgcgcgtgag gactggacga
     1621 tcctgcgcgc gttcagcgat gtggtgggcc acaggctgcc ctatgattcg atcgatgcgc
     1681 tgcggaagcg catggagcag gtgaacccgg tgtttggccg cattggattt ctgccgcgtt
     1741 tcggtgcgac cgatcagacc ggaccggctg gcgatcccac cgtgctgtca gatgtgccgt
     1801 tcgaaccggc ggtgctgaat tattatcaga ccgatccgat cagccgcgcc agcgccacga
     1861 tggcggcgtg tacgtcgacg caactggccg aggcggcgga atagcgcaat ggaagccttc
     1921 ttcctccaca cgatcatcgg ccgcctgatc ctgaccgtgc tcgaagccct gttgctgctg
     1981 gtgccgctgc tgatcggtgt cgcctatttg acctacgccg agcgcaaggt gctggcagcg
     2041 atgcagttgc gcaaaggccc caacgtggtg gggccgttcg ggctgtggca gccgttcgcc
     2101 gacgcgatca agatgatcat gaaggagacg gtgatcccgg ccggggcaaa ccgtctgctg
     2161 ttcctgctgg caccgatgct gacgatggga ttggcgctga tcgcgtgggc ggtgatcccg
     2221 gtcgccgatg gatgggtgaa tgccgacatc aatgtcggca tcctgtacct gttcgcgatc
     2281 tcgtcgcttg gtgtttacgg catcatcatc gcgggctggg cgagcaattc gaaatacgcg
     2341 tttctgggcg cgatgcgcag cgcggcgcag atggtgagct acgaagtctc gatgggcttc
     2401 gtgatggtat cggtgctgct gtgcgtcggc tcgctgaacc tgaccgacat cgtgcagggg
     2461 cagaaccact actggttcgg cttcatcccg gcctggtacg cgatcccgct gtttccgatg
     2521 ttcatcgtgt tcttcatctc ggcactggcg gagacgaacc ggtcaccgtt cgatctgccg
     2581 gagggtgaaa gtgagatcgt cgcgggcttc ttcgttgaat atagcgcgat ggcgtttgcg
     2641 ctgttcttcc tcggcgagta cgccaacatg atcctgatga gcgggatgac gacgatcctg
     2701 ttcctgggcg gttggctgcc gctgtttccg gtggcgccgt tcatctggat cccaggcgtc
     2761 atctggttcg tgctgaaagt ctgcttctgc ctgttcgtgt tcctgtgggt gcgcgccacc
     2821 ttcccgcggt atcgctatga ccagctcatg cggcttggct ggaaggtgtt cctgccgttg
     2881 tcgctgctgt ggctggtgat caccggcggg gcgctgatgc tgttcggatg gctgccccat
     2941 gcgtgaggga atctgctgat ggcctttctc gaccgcaccg cgcgcagtct gctgctaggc
     3001 gagctcgtcg ccgggctcgg gctgacgctg cgctacttct tcaagccgaa ggtgacgatc
     3061 aactacccgt atgaaaaggg gccgctttcg ccgcggttca agggcgagca tgcgctgcgc
     3121 cggtatccga acggcgaaga gcgctgcatt gcctgcaagc tgtgcgaggc gatctgtccg
     3181 gcccaggcga tcaccatcga ggccgaacca cgtgccgatg ggtctcgccg taccacgcgt
     3241 tatgacatcg acatgacgaa gtgcatctac tgtgggttgt gcgaggaagc ctgcccggtg
     3301 gatgccatcg tggaagggcc gaatttcgag ttcgccgccg aaacgcgcga ggagctgctc
     3361 tataacaagg agaagctgct ggataacggc gatcgctggg aagccgaaat cgcgcgccgg
     3421 ctggaactgg acgcgcccta ccgatgatcg caaccgtctt cttctacatc ttcgcggtag
     3481 tcctgttgat ctcggcggcg atggtggtga gttcgcggaa tccggtgcat tcggtgctgt
     3541 tcctgatcct gtcgttcttc aacgcggcgg cgctgttcct gattgcgggt gccgagttcc
     3601 tcgcgatgat cctgatgatc gtctatgtgg gcgcggtcgc ggtgctgttc ctgtttgtcg
     3661 taatgatgct cgacatcgac tttgccgagc tgcgcggcgg atttcagcgg tatctaccga
     3721 ttggcgcgac ggtgggagcg gtgctgttca tcgaactggc gctggtgatc agcggatgga
     3781 agttcgctcc gggtgcgccg acgctacggc tcgcgccggt gccggcgcag gtgagcaata
     3841 cggcggcgct ggggcggttg ctctacacgg actatgtgtt cctgttccag gcagcgggac
     3901 tgttgctgct ggttgcgatg atcggcgcga tcgtgctcac gctgcgggat cggaagacgt
     3961 cgcggcatca gatcatccgg gtgcagacgg aacgcacggt ggccgagaca ttggagatgg
     4021 tcactgtgtc gattggtgcc ggtgtgaagg atatcggcat cttcgaaccg aaggtggccg
     4081 aacaggagga gcccgagtca gcggaggaga cgcaccatgg gcactgaagt tggcgttggc
     4141 cattacctcg tggtgtcggc gatcctgctg gtgcttggca tcttcggcat tttcctgaac
     4201 cgcaagaacg tcatcatcat cctgatgtcg atcgagctga tcctgctcgc ggtgaacctg
     4261 aatctggtgg cgttctcggc ctcgctgggt gacctggtgg gacaggtgtt cgcgatgttc
     4321 gtgttgacgg ttgcggctgc cgaggcggcg atcgggttgg caatcgtggt gatttacttc
     4381 cgcaaccgcg gctcgatcca ggttgaggac gtcaacctga tgaaggggtg agatgatcta
     4441 cgccgtcgca gtgttcgcgc cgctgttcgg cgctttggtt gccgggctgc tggggcggtc
     4501 catcggcgat cgcgccgcca tggcggtttc tgtgctgtgc atgctggtcg cggcggcact
     4561 tggcccgatt gcgtgcttcc agcttgttgg cggcgatgcg tcggcaggca cggtgtcgct
     4621 cggcacctgg gtcgacgcag gccatttcca tgcatcctgg gcactgcgct acgacacctt
     4681 gtccgccgtg atggtagcca tggtgtgctt cgtggccatg ctgatccacg tctattcgat
     4741 cggctacatg gcgcacgacg cgtacccgcg ctatcgcttc ttctcctacc tgtcgctgtt
     4801 caccttctcg atgctgatgc tggtgacggc ggacaacctg ctgcagctct tcttcggctg
     4861 ggaaggcgtg gggctctgtt cgtacctgct gatcggctac tggtatgaca ggccatctgc
     4921 gaatgccgcg gcgatcaagg cattcatcac caaccgtatc ggcgacctgt tttttgctgt
     4981 cggcatcgcg ctgatcttct tcaagttcgg ctcgatcgag ttctctacga ttttccccgc
     5041 ggttccgcag catggagctg acacttactc ggtcctgggc ggcacctatc ctgcgttcga
     5101 ggtgatcggt gtgctgctgt tcatcggcgc gatgggcaag tcggcacaga ttggcctgca
     5161 cgtgtggttg cctgacgcga tggaaggccc gacgccggtt tccgcgctga tccacgcggc
     5221 gacgatggtc accgccggcg tgttcctggt cgcgcgcatg tcgccgatct atgagttcgc
     5281 accgttcgcg cttgggctgg tcaccttcat cggcgccacg acggcgctgt tcgccgccac
     5341 cattggctgc gtgcagaacg atatcaagcg ggtgattgcg tattcgacgt gttcgcagct
     5401 cggctatatg ttcgctgcag caggcgtcgg cgcattccag gtgtcgatct tccacctgat
     5461 cacccacgct ttcttcaagg cgctgctgtt cctcagcgcc ggctcggtga tccacgcgat
     5521 gtccgacgaa caggacatgc ggaagatggg cggcctctgg cgcatgattc cctggacctg
     5581 cgcgatgatg tggataggca gcctggcact cgcgggcatc ccgccgtttg ccggctacta
     5641 ctccaaggat gcgatcatat cggccaccta cgcgtccggt accggcatcg gcatgtatgg
     5701 cttcatctgc acagtgctcg cggcgttgct cacggcattc tactcgtggc ggttgctgtt
     5761 catgacgttc aacggcaagt cgcgggccga tcatcacgtg ctggaacacg tgcacgagag
     5821 cccgtgggtg atgatcgggc cgctgctcgt gctggggacc ggcgcgatcg ttgccggcaa
     5881 gctgctggat ggcacgttca tcggcgaggg ctggcaggag ttctggaacg gcgcgatttt
     5941 caacgcgccg aacaaccacg tgctggcagg cctcgagcat gtgccgggat gggtggaaat
     6001 gtcgccgctg gtcgtcggcc tgctgggcat cgcgcttgcc tatgtgatgt acatcgccaa
     6061 cccgctgctg ccgatgcggc tcgcgaccat gttccgcccg atctacctgt tcctgctgaa
     6121 caagtggtat ttcgacgagc tgtacgacgc gatctttgtc caaccggcga tccggctggc
     6181 gcggatgcta tggcagaccg gtgacgccac gatcatcgat ggcgtgccca acgggcttgc
     6241 cgagatcacg gtcgatggct cgcggcaggc ggtgaaaatc cagaccggtt cgctcgctgt
     6301 ctatgcgttc gtcatgctca taggcgtcgt ggtgctggtc ggcctcttcc tggtgtcacg
     6361 atgaacgcag ccggcttccc gatcctgtca ctgatcatct ggctgccgct ggtcggcggc
     6421 gttatcatca tgatggtgcg cggcgacgag gagactgtcg cctcgaacgc acgctggacg
     6481 gcgctgtgga ccagcctgat cgtgctggtg ctgtcgctgg tgctgtgggt gaagttcgac
     6541 accggcgagg cggggttcca gttcatcgag gaaatcccgt ggctccccga atatggtgtc
     6601 ggctacaagc tgggcgttga cggcatttcc gtgctgttcg tgctgctttc cacgctgctg
     6661 acgccgatat gcatcctggc gagctgggag tcgatcaagc gctcggtgcg cgagtacatg
     6721 ctgaccttcc tcatcctgga aacgatgatg gtggggatgt tctgcgcgct cgacttcgtc
     6781 gtgttctacg
//
LOCUS       DATGEM010000249         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_36790, whole genome shotgun sequence.
ACCESSION   DATGEM010000249 DATGEM010000000
VERSION     DATGEM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36790"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..1425)
                     /locus_tag="VKQ27_07920"
     CDS             complement(364..1425)
                     /locus_tag="VKQ27_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLY88895.1"
                     /translation="MAGEPPQDDRTEAATPRRLQKAREEGHVPVSRELSTFAGLAAVT
                     LVLMMAGPSTLHDLALRLGVILSRAHELGGGSALRLAGIAGLRAAAPFVLAAMLAGSA
                     AVLLQTRFLLSARSLWVDFSRISPRAGAKRLLGPDSVIEAGKSLVKIAVLGFALWYVI
                     LSDLSALLRVPFVEPNRLLPSVAGPVLHVILTVLVAQAVITAVDFFWVVMRHSRSLRM
                     SRHDIMEEQKETEGDPHIKARLRQIRVLRARKRMLAAVPKATVVITNPTHYAVALSYD
                     RAKHAAPRVVAKGVDTLAARIREVAQDNLVPIVANPPLARALYQVELDSEIPAEHFQA
                     VAEIIAYVWRLARPWPAAA"
     gene            complement(1432..2211)
                     /locus_tag="VKQ27_07925"
     CDS             complement(1432..2211)
                     /locus_tag="VKQ27_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLY88896.1"
                     /translation="MTGEDAALLATLPAWAFAFVLVVARVGAAMSLLPGLGEAESPMI
                     VRVGLALGITVLLLPGLAPSIPKVPEASMQAALMVAAEIITGLWLGWLARLLVLALPI
                     AGQFISYMLGIANVLQPDPELGSQATPLERMFDVGAAVVILASGLYALPLMALAGSYG
                     LVPAGTLLPTADTAQTAVRAVGMSFALAVRLASPFLLVSIVWHVGTGLLARLVPRMQV
                     YFVAMPGQILGGLALLAASAGALLAAWEGSVQAGFDLLPGH"
     gene            complement(2208..2480)
                     /locus_tag="VKQ27_07930"
     CDS             complement(2208..2480)
                     /locus_tag="VKQ27_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HLY88897.1"
                     /translation="MNDADVALLMRDGILVMLKLGGPPLLVALVVGLLVALLQAVTQL
                     NEATLAFVPKVLALCAALFLLGPFMLSTLSSYMHLLIDRLVAVGGS"
     gene            complement(2488..2799)
                     /locus_tag="VKQ27_07935"
     CDS             complement(2488..2799)
                     /locus_tag="VKQ27_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HLY88898.1"
                     /translation="MTTIPTIVITPSTAADAYGRVDRGDGASAAAGGDFSATLTRAMQ
                     GAIDQFHAADAHAAQALTGGGSLTEVVTALSRAELTLQTTTAVRDRVLQAYQDIIKMT
                     I"
     gene            complement(2796..3203)
                     /gene="flgC"
                     /locus_tag="VKQ27_07940"
     CDS             complement(2796..3203)
                     /gene="flgC"
                     /locus_tag="VKQ27_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HLY88899.1"
                     /translation="MELDKALGIAASGMDAQTTRLRVIAQNLANQDTTGSSAGAEPYR
                     RKTVTFQNTLNKELGADTVQVTQVGHDQSDFPMRYDPSNPAANAAGYVKVPNVNSLVE
                     IMDMHEAERAYSANLAVMQSARGMLSRTIDLLK"
BASE COUNT          634 a         1095 c          957 g          543 t
ORIGIN      
        1 tcccagaggg cgaggctgag cttgccggcg gcgccagctg tgcggatatg tacgagcggt
       61 aaagcagtag ccgcagcgcc cagcttgaca gcgttacgac gcgatattgg tcgacccata
      121 gccatagacg aactcccttc gaccgcttgt tcggcggccg ttccaatgtt tgagttttgc
      181 gcgggggatg gcccgccgtc gacgatccta gagacggcac cgaagcggcg acctcggatt
      241 ccatcgacca gcacgccgag ctgtcagtaa cggaaaatta gcgataacac cagcccgtat
      301 cgttgtcgtc gtgattggca attggcaaat atcggcgcgc agttcgcgcc ctacgtgccg
      361 gtgtcacgcc gccgcgggcc acggccgcgc cagacgccag acatatgcaa tgatttccgc
      421 cacggcctga aaatgttccg ctggtatttc gctgtccagc tccacctggt acagcgcccg
      481 cgccagcggc gggttggcga caatcggtac gaggttgtcc tgcgccacct cgcggatacg
      541 agcggccagc gtatccacac ctttggccac cacgcgcggc gcggcgtgct ttgcgcggtc
      601 gtaggacagt gccaccgcat agtgcgttgg gttggtgatt acgacggtag cctttggcac
      661 agctgccagc atccgtttgc gggcgcggag cacgcgtatc tgtcgaagcc gggccttgat
      721 gtggggatcg ccctcagtct ctttctgctc ctccatgatg tcgtgccggc tcatgcgcag
      781 cgagcgcgaa tgccgcatca ccacccaaaa gaaatccact gccgtgatca ctgcctgggc
      841 caccagaact gtcaggatca cgtgcaggac tggcccggcc acgctaggca ggagccggtt
      901 tggttcgaca aacggaacgc gaaggagtgc cgacagatcg ctcaggatca cgtaccacag
      961 agcaaacccc agcaccgcga ttttcaccag cgatttgccg gcctcgatca cgctatccgg
     1021 accaagcaac cgcttcgcgc cggcgcgcgg actgatgcgc gagaaatcca cccaaagcga
     1081 tctggcgctg agcaggaagc gcgtctgcaa caggaccgcc gccgaacccg ccagcatcgc
     1141 cgcaagcacg aacggcgcgg ccgcgcgcag ccctgcaatg cctgccaggc ggagcgccga
     1201 acccccgccg agttcgtgcg cgcgcgacag gatcacgccc aagcgcagcg ccagatcgtg
     1261 caatgttgac ggcccggcca tcatcagcac cagcgtcacc gcagcgagcc ccgcgaatgt
     1321 gctcagctct cgtgagaccg ggacgtgtcc ttcctcgcgt gccttttgca gccggcgggg
     1381 cgttgcggct tccgttctgt cgtcctgtgg aggctcgcct gccatggcag ctcagtgccc
     1441 tggcagcagg tcgaagcctg cctgcacgga tccctcccag gcggccaaca acgcgcccgc
     1501 acttgcggcg agcagtgcaa gccctcccag aatctgacca ggcatggcaa cgaaatagac
     1561 ctgcatgcga gggaccaggc gcgccagcaa tcccgttcca acatgccaga cgatcgaaac
     1621 cagcaggaac ggcgaggcca ggcgcaccgc cagcgcgaat gacatgccta cagcgcgtac
     1681 tgctgtctgc gcagtgtcgg ccgtcggtag cagtgtaccg gctggaacca acccgtagct
     1741 gccggccaac gccatcagcg gcagtgcata cagcccgctg gcaagtatca cgaccgctgc
     1801 gccaacgtcg aacatccgct cgagcggggt ggcctggctg cccagttccg gatcaggctg
     1861 caggacattc gcaatcccca acatgtagga aatgaactgt ccggcgatcg gcagtgccag
     1921 caccagcagt cgcgccagcc agccgagcca caggccggtg atgatctccg cggcaaccat
     1981 aagtgccgcc tgcatgctcg cttcaggcac cttcggaatg gatggcgcga gccccggcag
     2041 cagcaacacg gtgatgccaa gcgcgagacc aacgcgcacg atcatcggtg attctgcctc
     2101 gccaagcccg ggcagcagcg acatggccgc gccgacacgc gccacgacca acacgaatgc
     2161 gaacgcccac gccggcaggg ttgcgagcag ggccgcatcc tcgccagtca tgagccaccg
     2221 acagcgacca atcgatcgat caacaggtgc atatagctcg acagcgtcga cagcatgaac
     2281 ggtccgagca gaaacagggc cgcgcacaac gcgagcactt tcggcacgaa tgccagcgtt
     2341 gcctcgttga gctgtgtcac cgcctgaagc agtgcgacca gcaacccgac aacgagagcc
     2401 accagcagcg gcggcccgcc cagcttcagc atcaccagaa tgccgtcccg catcagcagc
     2461 gcgacgtcgg catcgttcat tgcgacctca gatcgtcatt ttgatgatat cctgatacgc
     2521 ctgcaagacg cggtcgcgta ccgcggttgt ggtctgcaat gtgagttcgg ctcgcgacaa
     2581 cgcggtaacc acctcggtca agctgccgcc accggtcagg gcctgtgccg catgcgcatc
     2641 cgcggcatgg aattgatcga ttgctccctg catcgcccgc gtcagcgtcg cgctgaagtc
     2701 gccgccagcc gcggcactcg ctccgtcgcc ccgatcgaca cgcccataag catcagcagc
     2761 ggtcgaaggc gtgataacaa tggtggggat cgtcgtcatt tgagcagatc gatcgtgcgc
     2821 gaaagcatgc cgcgtgcgct ctgcatcacc gcgaggttgg cgctgtatgc acgctccgcc
     2881 tcatgcatat ccattatctc gaccaggctg ttcacattcg gcaccttcac atagcctgcc
     2941 gcgttcgccg ccgggttcga cggatcataa cgcatcggga aatcgctctg gtcgtgcccc
     3001 acctgcgtca cctgcacagt atcggcgccg agttccttgt tcagtgtgtt ctgaaacgtg
     3061 accgtcttgc gccgatacgg ttcggcaccc gctgacgaac cggtcgtgtc ctgattggcg
     3121 aggttttgcg cgatcacacg caaccgcgtt gtctgcgcat ccataccact cgccgcaatc
     3181 cccaacgcct tgtcgagttc catgcgcgac accttccctg ctgactggc
//
LOCUS       DATGEM010000250        22369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_819269, whole genome shotgun sequence.
ACCESSION   DATGEM010000250 DATGEM010000000
VERSION     DATGEM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 22369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22369
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819269"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VKQ27_07945"
     CDS             complement(<1..860)
                     /locus_tag="VKQ27_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88900.1"
                     /translation="MRRLLLAILLISTSPAFAADLALKRVMLSSAGVGYFEYEADVDG
                     AATLGLDVPLDQVDDVLTSLVVFDSAGGVGTVELPGRDNTRAEFGNLPFGPNALNSPV
                     DYLNSLQGVEISVDGPRPMTGRIMHADRITESLPGPPPATVQRTRVTLMGASGMQQFV
                     LEDAESIQVTDPALRTRIGSALESLRREASLSMRHITLHSDGTGQRTVRVGYVAVAPL
                     WKASYRLVLPAKDGDPAHLQGWAVLENQSGTDWNGISLTLQYGNPVTFRQAIYRSYYV
                     QRPEVPVEILG"
     gene            911..1105
                     /locus_tag="VKQ27_07950"
     CDS             911..1105
                     /locus_tag="VKQ27_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88901.1"
                     /translation="MALFSAVTAEDMQTARIVTGVAMALFIGAGVVPGLRQRAVAIRW
                     ALLGVYLVACVAFVGYVLLK"
     gene            complement(1112..1675)
                     /locus_tag="VKQ27_07955"
     CDS             complement(1112..1675)
                     /locus_tag="VKQ27_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HLY88902.1"
                     /translation="MTESIVAEPAITQGLRYDPTTIALHWVTAVLVVLLWIIGQTVDF
                     PPTPALRVDYRSLHIVFGATLGIILLARLAWRLTRRDVPPPIDQGLLLVIARLTHWGL
                     YVLLVVAVGLGIGTTWTRGDSLFGLFSIPAFDPGNRALMRQIHSWHALAANTVLIVAG
                     LHAAAALFHHFVMRDATLRRMLPWQLR"
     gene            complement(1684..2640)
                     /locus_tag="VKQ27_07960"
     CDS             complement(1684..2640)
                     /locus_tag="VKQ27_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HLY88903.1"
                     /translation="MRNFSDLTEREVLALAIANEEEDGRIYQDIAERLREDYPGSAKV
                     FTEMAGEEGDHRRRLIELYQDKFGEHIPLIRRRDVRGFVEHPAIWHIEPFGLDKVRDL
                     AEDMELETLRFYRRAAARSQDASVRKLLGDLAEAEVHHERIAGELVEENLPASVRDEE
                     DRHQRRMFILRVIQPGLAGLMDGSVSTLAPVFAAAFATGQSWNAFLVGIAASLGAGIS
                     MAFAEALSDNGSLTGRGAPYIRGAITGLMTTLGGIGHTLPYLIPNFWTATTVAITVVI
                     VELAVISWIRWKYMDTPPFSAAFQVAFGGALVFATGILIGSS"
     gene            complement(2733..3344)
                     /locus_tag="VKQ27_07965"
     CDS             complement(2733..3344)
                     /locus_tag="VKQ27_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88904.1"
                     /translation="MAKAVAWTHSFAAPITSSQQSLALYFQEMLLWAASRAGTQLGAL
                     DVGSGGAHGMKMEERFLLDNPKPHRFYRASVGSTVERKVYTELKNLVLTDITPIVASG
                     DWAGVYQANLATQSDLVRITFAIEYPPACSPALPSVFRSSRPDIRLALGQGSDGAYYE
                     ALYDLTSEGQIGHVLTKGDNWVNKHSVAYIAEIVWMNDDIMQK"
     gene            complement(3366..4697)
                     /locus_tag="VKQ27_07970"
     CDS             complement(3366..4697)
                     /locus_tag="VKQ27_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLY88905.1"
                     /translation="MSSRVLHRVGGAPPIATGGDGIYLLRADGVRIIDGSGGAAVACL
                     GHGNRRIAEAISRQAATMAYAHTGTFSSQPAEDLAHIVLDGEPGGLSHAYFCSSGSEG
                     TEAALKLARQYFVEIGQPQRTHFIARRQSYHGNTLGALGAGGNMMRRALYEPILAPAF
                     SLVSPCFAYRFKRNDESDQQYVDRLADELEAEFERLGPDTVIGFLAEPVVGATTGCVA
                     ALPSYFKRVREICDRHGALLILDEVMCGMGRTGTMHAWEQEGISPDLQIVAKGLGGGY
                     QPIGGVLIHSRIVQAINAGSGGFMHGHTYQAHPVACAAALEVQRIIRDDHLLDNVRAM
                     GTRLENGLIERFGNHRHVGDVRGRGLFWALEFVSDHATKQVFDPKLKINERVKREALA
                     RGLAIYPMGGTIDGKQGDHVIVAPPYIATGADIDTIVDRLGAAVDAALSEV"
     gene            4789..5676
                     /locus_tag="VKQ27_07975"
     CDS             4789..5676
                     /locus_tag="VKQ27_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY88906.1"
                     /translation="MADADIAAIRTMLASQPRPSGPAERRQRLDSFGSALGVPGDARL
                     EMVQINSVQAEWSATPAADPASAVLYLHGGGYMAGSIVSHRYVAIEIGRAACARTLAI
                     DYRRAPEHPYPAQLDDALAAYRHVLDQGLTPGKIAVGGDSAGGNLTLALLIALRTQGL
                     PLPACGWVVSPWSDLTASGATMQSKASVDPMIQKPYLLDLAQSFAGGRDLRDPLISPQ
                     LADLTGLPPLLIQVGSEETLLDDSVMLAGRAGAAGVATTLEIWPDMIHAFTMFFPRVA
                     ASRRATANAGRFIRRHFGD"
     gene            complement(5848..6933)
                     /locus_tag="VKQ27_07980"
     CDS             complement(5848..6933)
                     /locus_tag="VKQ27_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88907.1"
                     /translation="MEFGYFTMPSHPPERGLKAGHDWDLQTLRWLDELGYKEAWIGEH
                     YTAPWEPHPSPDLLIAQALTQTKRLRIGPGGFLLPYHHPAELAARVAMLDHLSEGRLN
                     FGVAASGLPTDWAMFQVDGTAGVNREMTREALDIILRLWSDEPTFDYKGQFWQVAKPE
                     PMFGFLKPHIYPLQKPHPPIGVAGFSKQSDTLKLAGEKGFLPLSLNLNPAYVASHWEA
                     VEIGAARTGRTPKRADWRLVREVFVADTDEEAMRLSAGGMMGRMMREYFLQLLGNFGF
                     LEYLKHDPNVADSDVTPEYCAKHNWLIGSPATVAEKLERIYREAGGFGGLLVFGFDYA
                     DNPQAWHNSLRLLIEEVGPRVAHLTPS"
     gene            complement(7180..8370)
                     /locus_tag="VKQ27_07985"
     CDS             complement(7180..8370)
                     /locus_tag="VKQ27_07985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY88908.1"
                     /translation="MPDELPLAGIRIIDLTRVLSGPFCTMLLGDLGADIIKVETPGEG
                     DTIRHQGAGRDGLSWYFANYNRNKRSITLNLRSDEGRDILAGLIRQSDVLMDNYRPGV
                     LSAMGFTDERLRELNPRLVRGSVTGFGTTGPYRDRPSFDFIAQAMSGFMSVNGAADHP
                     PLRAGPPVSDLIAGLYAALGIVAGLLREGRTRQGGTASVSLTGGLISFLGFLATDYLA
                     TGNLPKRTGNDHPIAAPYGLFRTADGEIAIAPAGEAMYLRLLRALDAESLRERPEFAT
                     NTQRATNRAAINAEIEARTTRETSEHWIRVLNEAGVPCSPVMQLDQVFADPQVIDQRS
                     VVTVAHPGHGDVSMLGPALHVDGAPLPVRRPAPALGEHTAEVLGELGYSTEAIVRLRE
                     RGVA"
     gene            8559..8936
                     /locus_tag="VKQ27_07990"
     CDS             8559..8936
                     /locus_tag="VKQ27_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY88909.1"
                     /translation="MCAADDGPIKIGVVSEEAAIVGQAISQGAELAVEIITYDNHSSA
                     SGAVRGYQRAVQQDHVSAVVTTFISEVALATEPWSARLHMPTITPAAASDLISKQLHD
                     EQDGVSFIRQGTGGGEVARVRSS"
     gene            complement(9068..9850)
                     /locus_tag="VKQ27_07995"
     CDS             complement(9068..9850)
                     /locus_tag="VKQ27_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88910.1"
                     /translation="MNDDSARRAAWSKVPEITLTFWVIKIAATTLGETGGDSVTMTLN
                     WGYLAGTALFLALLVALVLAQIRAERFRPFLYWATIVASTTFGTTMADFADRSLGIGY
                     TGGSLLLLACLLTVLLLWYLSERTISVDTVTTPRVEAFYWAAITFSQTLGTALGDWAA
                     DTGQLGYGGGALVFAAGLAIVAVLYVWTNASHVLLFWAAFILTRPLGATVGDFLDKPL
                     SDGGMALSRPLASAVIAAFIVMCVLVLPQRPGRHPGVPATAG"
     gene            complement(9952..12231)
                     /locus_tag="VKQ27_08000"
     CDS             complement(9952..12231)
                     /locus_tag="VKQ27_08000"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HLY88911.1"
                     /translation="MRASRPDIETLLTARWGVWLGSAALVLAGVFLIRYAVDQGLLGP
                     GPRCVLAGLLGVALIAAAEWLRGREILHPAVADNASPGLAAGGVAILFGASYGASAMY
                     GLVPPQAGFVLMAAASVAGIALSLRYGQVVAAIGLLGAFVTPALVNTENPSIPGLYGY
                     LLFVTAAALAVVRYTAWIWLGWATTIAGAIWVALAILGGAGSDVWAPALFVPAAAALN
                     LGLLPAAALNHPIGRKLAFIPCAALGAAGLSLAIDDQTAITRIGVLLLAPLLVWHASR
                     EPRLRLLPFLAAALFLALLATWNTDGSEQSLLLTAALVSGFFAASGLALERRPDTQLA
                     WASLTASIPVLTLAVCYALVTEFQPRFDWAAFAILLTAGLIGTTTRALRDNTRQRAGI
                     HAAGAVAALALGCGMFLRDEWLTIAVSLFLPALAWIAAEAELPALRRVALAVATIVLV
                     RLLLNWYVLDYGFGQWPIVNDLLIAYGVPGIAFAVAAALFRRDRDDATVGVLEAGSAA
                     FLTVLVALEVRHTFGPPAGLATPELGFPEAALHVSSLGVVCLTTLRIAERLQRPMLHI
                     AWKVQGALALIGAVLLLVANPGLTNEPVGNAPLIDWLLPAYLLPALLAIAASRHPAVA
                     GVPKLRIVLEGYSLLAGLAWLTLEVRHLFNGGSIGFADIPIGDAELWAWSGAWLAYGV
                     IVMAIGIHTGIRHMRLAALGIVGLAAAKVFLVDMSSLVGLWRVVSFLGLGVVLIGLGA
                     AYRHLAGPPPPDAPNAVEP"
     gene            12346..12552
                     /locus_tag="VKQ27_08005"
     CDS             12346..12552
                     /locus_tag="VKQ27_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88912.1"
                     /translation="MLDGGGAQLAHEPAQFMGLPPNAQREEKAYHAQQPTRGSDQDKR
                     HYVVHSEPYVGHVTGCDRYGGLVK"
     gene            complement(12597..13397)
                     /locus_tag="VKQ27_08010"
     CDS             complement(12597..13397)
                     /locus_tag="VKQ27_08010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY88913.1"
                     /translation="MGSISPEAIESVQQLSDTWRTFVLDRGVGDVSERPGMAIRWANS
                     KFPFWNCITFTDLGCGSRLLDERFAKATAYMRRKSRPGLIWLFDDLLDANARAGLPAA
                     AERAGLSLLFPGFGMAGDILPIAAPHHPQLDFVRVKTEEQLMAFADLNSRAYAMPLEH
                     GRDGLAGSVLWKSGMFSYLGLQDGVPVSAAATIATGNCLFLALVATAPEARRKGYGEA
                     TVRKALFEGGRATGLTRTVLHATLAGAPVYERIGYRKVASFGFYGLKE"
     gene            complement(13454..13585)
                     /locus_tag="VKQ27_08015"
     CDS             complement(13454..13585)
                     /locus_tag="VKQ27_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88914.1"
                     /translation="MLAIFSPGGIEGFFRQLPAVDRDGIKTVAAVYGCRMVGAPLHT"
     gene            complement(13613..13906)
                     /locus_tag="VKQ27_08020"
     CDS             complement(13613..13906)
                     /locus_tag="VKQ27_08020"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY88915.1"
                     /translation="MAVTTAPRDANRVPIEWLQWLPGARVAIRVASDITRGSFTMLEE
                     EAGPRSGPPLHVHQNEDEHFIFLEGVVRIICEERSEDVTAGMTLAVPRGVPHA"
     gene            complement(14145..14561)
                     /locus_tag="VKQ27_08025"
     CDS             complement(14145..14561)
                     /locus_tag="VKQ27_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY88916.1"
                     /translation="MTRRLTQQDYRLLSEFRYQLRCFLEFSEAAAARAGLKPRQHQAL
                     LAIKGAREGAIPSVGYLAERLRIRHHSAVELADRLAEAGLITREHDPEDRRRVRLNLT
                     PTAEERLAKLSANHLDELSRLRPALLELLLPDQPKQ"
     gene            14634..16439
                     /locus_tag="VKQ27_08030"
     CDS             14634..16439
                     /locus_tag="VKQ27_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLY88917.1"
                     /translation="MTDTALPGAGNGVADHATRAGIRHLGDFTTSPRVLLIAAIALVV
                     GTGGVIGGVILLKLIQLCTNLAYNGVFSLADLKPAGSPLGVASVLVPVIGALIIGFMA
                     RFGSEKIRGHGIPEAIEAILLGRSKLDLKVAILKPLSSAISIGTGGPFGAEGPIIMTG
                     GAIGSLIAQLLPVTDAERKTLLVAGAAAGMTTVFGTPIAAVMLAVELLLFEWTPRSFV
                     PVAVAAIVAELERTWLGLASPLFPFAGGMAITLVAALYWVLVGLLSGALSGLLTRLVY
                     LWEDAFLKLPIHWMWWPALGGVVVGIGGLIDPRALGVGYDNIAQMLQGGMPPASSLIL
                     LAVKAIIWAVALGSGTSGGVLAPLLIMGGGMGAALAHLLPVASPGFWALLAMAATMGG
                     TMRSPLTATFFAVEITGNTHVLLPLITACMAAHVVTVLIMRRSILTEKVARRGHHLSR
                     EYRVDPFALTRARDIMTTGVETVQDSMTLHQVARFMTQPDTRHPSFPVVDAARRVLGI
                     VDPPTVMRWRRAGEQRCRTLAELMARTKAVVAYPDEYLDEVVARMTQANVAHIPVVAR
                     EDGRLVGYIGWKDLMNVRARLQAEERERVVLYRVR"
     gene            complement(16546..19071)
                     /locus_tag="VKQ27_08035"
     CDS             complement(16546..19071)
                     /locus_tag="VKQ27_08035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLY88918.1"
                     /translation="MDAAAQAEHPGLTAGEAAERLRHEGPNELPRRGRRNLPHILHDV
                     LAEPMFALLLGAGVIYLLLGDHLEALLLLIFASLSVAIAVVQEARSERVLEALRDLTS
                     PRALVIRDCQRQRIAGREVVRGDLLAVSEGDRVAADATLIEAHDLQIDESLLTGESVP
                     VRKRRGLGTEDGMHPGGDDSPQVYSGTLVVRGQGLAIVRSTGPRSEIGRIGQTLQAIE
                     TAPANLSRQTSALVRAAAAVGLVCCLLVILLFGLLRGSWLDALLAGIALGMSMLPEEF
                     PLVLTVFMVMGAWRMSQARVLTRRAAAIETLGEATVLCTDKTGTLTENRMAVAVLQAT
                     ASPPTVLRGTEVPAHLHDLVHIAALASASNAYDPMDQATQSLAKAIGSCTTDGNLVKV
                     YGVQPDRLAFIQAWQHSADGALQIAAKGAPEAIAWLCRLDDAPRLRLLRDADQLAAGG
                     MRVLAVAAASTTGGAPEDPAAYDFHLVGLLGLADQLRASVPAAVAQCRAAGIRVVMIT
                     GDYPVTARAIAQRAGLDCDRVLDGETVAQMDDAEFLAAARQTTVFARVMPNEKLRIVR
                     ALRAAGEVVAMTGDGVNDAPSLRAADIGIAMGGRGTDVAREAASLVLLDDDFGSIVQT
                     IRLGRRIYDNLRNAMGYIIAVHVPIAGLALLPLLTGLPLIFGPVHIAFLEMVIDPVCS
                     MVFEAEAEEADIMRRPPRAAGTPLLSPVLITWGLVQGIVAFVFLASLYTITIRSGLPD
                     GDARALTFVSLVLVDLGLIMVNRNFNASLRDLVGQKNRALIWISGITVALLVLVLGTP
                     LGRELFRFGPLHPDDLSLVALVVIGTVAILEAAKRFWRARLTN"
     gene            complement(19085..19561)
                     /locus_tag="VKQ27_08040"
     CDS             complement(19085..19561)
                     /locus_tag="VKQ27_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLY88919.1"
                     /translation="MAPELGRRLLLGTIVAGSAAIVAVAQPTVGRGPGMMGGGMGPGM
                     MGGYWNTASYLDGLKTRLGITAGQEPVWKDYSDTVTGVGEQMQALHQTMFEAMGTASW
                     QERRNLMDSMFQTRQQASDTVHQAAIKLVAALAPAQKERAQQILPGLAYGHGMMMR"
     regulatory      19695..19810
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19965..21095
                     /gene="gcvT"
                     /locus_tag="VKQ27_08045"
     CDS             19965..21095
                     /gene="gcvT"
                     /locus_tag="VKQ27_08045"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HLY88920.1"
                     /translation="MIENALPDDPTPKALKTTPLDAWHRARGARMVEFAGYSMPVQYQ
                     GVIAEHLQCRAKAALFDVSHMGQATLAGPTAAAALESLVPGDIVGLKPGRQRYTLLTN
                     EAGGIKDDLMVANLGGDKLFLVVNAACKDVDFTHIGAHLPAGVQLQPHEDRALLALQG
                     PAAAAVLARLGQEAAGQPFMGVLNAMLGGIPCLVSRSGYTGEDGFEISVPADRAEALA
                     DRLVADPEVAPAGLGARDSLRLEAGLCLYGNDIDELTTPVEAGLTWVIGKRRRTSWDF
                     PGAAAIRDQLENGAVRHRVGIRPDGRAPARAQTDIVAGDGTAAGIITSGSFSPSLNAP
                     IAMGYVRKDLVADGTALHLIVRGKPLPARVVPLPFVPHRYAH"
     gene            21111..21500
                     /gene="gcvH"
                     /locus_tag="VKQ27_08050"
     CDS             21111..21500
                     /gene="gcvH"
                     /locus_tag="VKQ27_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLY88921.1"
                     /translation="MAKPPSDIRYTKEHEWIRLDGDIATVGITDHAQEQLGDVVFVEL
                     PEIGRDVAADEACAVVESVKAASDIYAPLTGKIAETNPTIIDDPSLVNSDAEGEGWFF
                     RIEIDDTDAFEALMDAAAYDEYLETLD"
     gene            21500..>22369
                     /locus_tag="VKQ27_08055"
     CDS             21500..>22369
                     /locus_tag="VKQ27_08055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013509.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HLY88922.1"
                     /translation="MDVWAELAELEAADGFVARHIGPSESDIAAMLHAVGAATLDDLA
                     AKTVPPAIRTNQALELPPPIDEAAVMAELRVLAAKNGGAKSLIGMGYHGTHMPPVIQR
                     NVLENPGWYTAYTPYQAEIAQGRLEALLNFQTMISELTAMEIANASLLDEGTAAAEAM
                     AMAHAISKTKSDVLAVATDLHPQTRAVLATRAKPIGITLVDFAPSDLAAIAAAQPFAV
                     VLQYPGTTGAIRELSPEIGAAHEVDALVIVCADPLALALLTPPGEMGADIVVGSSQRF
                     GVPIGFGGPHAGYM"
BASE COUNT         3909 a         6660 c         7929 g         3871 t
ORIGIN      
        1 ccaaggatct ccaccggcac ctcgggacgc tgcacataat agctgcgata gatcgcctgg
       61 cggaatgtca ccgggttgcc gtactgaagc gttagggaga tgccattcca atccgtgccg
      121 gattgattct ccagcaccgc ccagccctgc aggtgggcgg gatcaccgtc cttggcggga
      181 agcacgaggc gataggacgc cttccacaag ggcgcgacgg cgacataacc aacgcgcacc
      241 gtgcgctggc cggtgccgtc gctgtgcagc gtgatgtggc gcatgctgag gctggcctcg
      301 cggcggagcg actccagcgc ggaaccgatg cgcgtgcgca gggcgggatc ggtgacctgg
      361 atgctctcag cgtcttccag cacgaactgc tgcatgccgg acgcgcccat cagagtgacg
      421 cgggtgcgtt gcaccgttgc gggcggggga ccgggcagtg attcggtgat gcgatcggca
      481 tgcatgatgc ggccggtcat cgggcgtggt ccgtccacgc tgatctccac gccttgcagg
      541 ctgttcagat aatcgaccgg cgagttcagc gcgttgggcc cgaagggcag gttgccgaat
      601 tccgcgcggg tgttgtcacg gccggggagt tcgacggtgc cgacgccgcc ggcgctgtcg
      661 aacacgacga gcgaggtgag cacgtcgtcc acctgatcca gcggcacgtc gaggccgagg
      721 gtagcggcgc catcgacgtc ggcttcgtat tcgaagtagc cgacgccggc ggaggacagc
      781 atcacccgct tcagcgccag atcagccgcg aaggccggcg acgttgagat cagcagaatc
      841 gccagcagca atcgtcgcat tgcgttcctc ccacccggtg ccggagaata gcacggcagg
      901 aggcgaaaag gtggcgctgt tcagtgccgt gaccgcggaa gacatgcaga cagcacgtat
      961 cgtcacgggc gtggcgatgg cgctgttcat tggagcagga gtggtgcccg gattgcggca
     1021 gcgggcggtg gccattcgct gggcgctact tggagtctac cttgtggcgt gcgtggcgtt
     1081 cgttggttac gtgctgctga agtaggatcc gttatcgcag ctgccagggc agcatccggc
     1141 gcaatgtcgc atcgcgcatg acaaagtggt ggaacagagc cgccgcagca tgcaggcctg
     1201 caacgatcag cactgtgttg gcggcgagcg cgtgccagct gtggatctga cgcatcaatg
     1261 cgcggttgcc tggatcgaag gcgggaatgc tgaacagtcc gaacagcgaa tcgccgcgtg
     1321 tccacgtggt gcctatgccc agtccaactg cgaccaccag caggacataa agcccccagt
     1381 gcgtgaggcg ggcgatgacc agcagcaatc cctgatcgat aggtgggggg acatcgcgcc
     1441 gtgtgagccg ccaggccaga cgggcaagca gtatgatgcc caacgtggcg ccgaacacga
     1501 tatgcagcga gcgatagtcg acacggaggg caggggtcgg tgggaagtcg acggtctggc
     1561 cgatgatcca gagcaatacg accaggacgg cggtcaccca gtgcaatgcg atggtcgttg
     1621 ggtcatagcg caggccctgc gttatggcag gttccgccac gatcgattcg gtcatggagt
     1681 cctctagctg ctgccgataa ggatgccggt ggcgaatacc agggcgccac caaatgccac
     1741 ctgaaatgcg gcggaaaatg gcggggtatc catgtatttc cagcgtatcc aactgatcac
     1801 ggccaattca acaatcacta cggtgatcgc gaccgtcgtt gccgtccaga agttggggat
     1861 caggtagggc aacgtgtggc cgataccgcc gagcgtggtc atcagaccgg tgatggcgcc
     1921 gcgaatatac ggtgcgccgc ggccggtgag gctgccgttg tccgaaagcg cctcggcaaa
     1981 ggccatggag ataccggcgc ccagcgaggc ggcgatgcca acgaggaatg cgttccatga
     2041 ctggccggtg gcgaaggcgg cggcgaacac cggggccagg gtggagacgg agccatccat
     2101 caggccggcg aggccaggct ggatcacccg caggatgaac atgcggcgct ggtgccggtc
     2161 ttcttcgtcg cgcacgctgg cgggaagatt ctcttcgacg agttcgcccg ctatccgctc
     2221 gtggtgcacc tcggcttcgg caagatcgcc gagcagtttg cgaaccgagg cgtcctggct
     2281 gcgcgcggcg gcgcgacgat agaagcggag ggtttcgagc tccatgtcct cggcgaggtc
     2341 gcgcaccttg tcgaggccga agggttcgat gtgccagatc gcggggtgtt cgacgaagcc
     2401 acgcacgtcg cggcggcgga tcagcggaat gtgttcgccg aatttatcct ggtaaagctc
     2461 gatcaggcgg cggcgatgat caccttcctc gccggccatc tcggtgaaga ccttggccga
     2521 gccgggatag tcctcgcgca ggcgttcggc gatgtcctga tagatccggc cgtcctcctc
     2581 ctcgttcgcg atggccagcg cgaggacttc gcgttcggtc aggtcggaga aatttcgcat
     2641 ggccgcaatg tggcaattgc gatggcccgg tcaagacggg tgcgagatga tgcgactgct
     2701 tcgcaacagc agatgtggcg tatttctcag ttttatttct gcatgatgtc gtcgttcatc
     2761 cagacgatct cggcaatgta ggcgaccgag tgcttgttga cccagttgtc acccttggtg
     2821 agcacgtggc cgatctggcc ctccgacgtg aggtcgtaca gtgcctcgta ataggcgcca
     2881 tcgctgccct ggccgagcgc gaggcggatg tcgggacggg aactcctgaa cacggagggc
     2941 aatgcgggag agcaggcggg cggatattcg atggcgaagg tgatgcggac gagatcggac
     3001 tgggtcgcca ggttggcctg atagacgcca gcccagtcac cgctcgcgac gatgggggtt
     3061 atgtcggtga ggacgaggtt cttcagttcc gtgtagacct tgcgttcgac cgtggagccg
     3121 acggaggcgc ggtagaagcg atgaggcttt ggattgtcga gcaggaagcg ctcttccatc
     3181 ttcatgccat gtgcgccgcc tgatccgaca tcgagcgcgc cgagctgcgt gccagcacgg
     3241 gaagcggccc aaagcagcat ttcctggaaa tacagcgcca gcgattgctg gctgctggtg
     3301 atgggcgcgg cgaacgaatg ggtccacgca acagccttgg ccatgcactg cctcctgggg
     3361 tcggactaga cctcggacag cgcggcatcc actgctgcac cgaggcgatc gacgatggtg
     3421 tcgatgtctg ctccggtggc tatataggga ggcgcgacga taacgtgatc cccttgtttg
     3481 ccatcaatcg tgccgcccat cgggtagatg gcaaggccgc gcgcgagggc ttcgcgcttg
     3541 acgcgttcgt tgatcttcaa cttcggatcg aagacttgct ttgtcgcgtg gtcgctgacg
     3601 aattcgaggg cccagaacag gccgcggcct cgcacgtcgc cgacgtggcg gtgattgccg
     3661 aagcgctcta tcaggccgtt ctcgaggcgg gtgcccatgg cgcgcacgtt gtccagcagg
     3721 tggtcgtcgc ggatgatgcg ctggacctcg agtgcggcgg cgcaggcgac ggggtgcgcc
     3781 tggtaggtgt ggccgtgcat gaagccaccg gagccggcgt tgatggcttg cacgatcctg
     3841 ctgtggatga gtacgccccc gatgggctgg tagccaccgc ccaggccttt ggcgacgatc
     3901 tgtaggtcgg gtgaaatgcc ttcctgttcc caggcgtgca tcgtgccggt gcggcccatg
     3961 ccgcacatca cctcatcgag gatgaggagt gcgccgtggc ggtcgcagat ctcgcgcacg
     4021 cgcttgaagt agctggggag ggcggcgacg cagccggtgg tcgcgccgac gacgggttcg
     4081 gcgaggaaac cgatgacggt atcggggccg aggcgctcga actcggcttc gagttcatcg
     4141 gcgaggcgat cgacatattg ctgatcactc tcatcgttgc gtttgaaacg ataggcgaag
     4201 cagggggaaa cgaggctgaa ggcgggggcg agtatcggct cgtagagggc acgtcgcatc
     4261 atgttgccgc cggcgccgag cgcgccgagc gtgttgccgt ggtagctctg gcggcgggcg
     4321 ataaagtggg tgcgctgtgg ctggccgatt tcaacgaagt actggcgcgc gagcttgagg
     4381 gcggcttcgg tgccttctga gccggaggaa cagaaatagg cgtggctcag gccgccgggt
     4441 tcgccatcga gcacgatgtg ggcgaggtct tcggcgggct gactactgaa ggtgccggta
     4501 tgggcgtagg ccatggtggc ggcctggcgg ctgatggcct cggcgatgcg gcggttgccg
     4561 tggcccaggc aggcgaccgc ggcgccaccg gagccatcga tgatgcggac gccgtcggcg
     4621 cggagaaggt agatgccgtc gccgccggtg gcgatgggcg gggcgccgcc gacgcgatgg
     4681 aggacacgac tggacatggc cgctttaagc ctctcgttga tgcggatcgt ccattgtcac
     4741 cgacgccaga aaatgtcacg cgtgaactgc gcagaactgg gaggcaccat ggctgacgcc
     4801 gacatcgctg ctatccgcac catgctggca agccagccgc ggccgagcgg tccggctgag
     4861 cggcgtcagc gcctcgacag ctttggcagc gcgcttggtg tgccgggcga cgcgcggctg
     4921 gagatggtgc agatcaacag cgtacaagcc gagtggtcgg ccacgcctgc ggcagatccg
     4981 gcatccgctg tgctgtatct tcatgggggt ggctacatgg cgggctcgat cgtgagccat
     5041 cgctatgtcg cgatcgagat aggccgcgcg gcgtgcgccc gcactcttgc gatcgattac
     5101 cggcgcgcgc cggagcatcc ctacccggca caactcgatg atgcgcttgc cgcctatcgc
     5161 cacgtgctcg atcaggggct gacacctgga aagatcgctg tcgggggcga tagtgccgga
     5221 ggcaacctca cgctcgcatt gctgattgcg ctgcgaacac aaggcttgcc gctgccagcc
     5281 tgtggctggg ttgtttcacc gtggagcgat ctgacggcga gtggcgcgac gatgcaatcc
     5341 aaggcctcgg tcgatccgat gatccagaag ccgtacctgc tcgacttggc acagagtttt
     5401 gctggcggca gagatctgag agatccgctg atctcgccgc agcttgccga tctgacgggg
     5461 ttgccgccgc tgctcattca ggttggttcg gaggaaacgc tgctggatga ttcagtgatg
     5521 ctggccgggc gcgcgggcgc cgccggtgtt gccacgacac tggagatctg gcccgacatg
     5581 atccatgcgt tcacgatgtt ctttccgcgt gtcgcggcat cgcggcgagc gacggccaac
     5641 gccggccgct tcatccggcg gcatttcggg gattaagcgg ggatcagact cggccgccgt
     5701 tgcggttggg ctcgcggtaa ctaggaaaat ggaaccgcaa aacacgcaga tgataccggc
     5761 ggagacgagt gagttgtacc agccctgcag gtgccgccgc catctgcgtg ttctgcggtt
     5821 cgattctcag gacatttgct cgattgtcta tgacggggtg agatgcgcca cgcgcggccc
     5881 gacctcctcg atcagcagcc gtagcgagtt gtgccacgcc tgtggattgt cagcgtagtc
     5941 gaagccgaag accagcaggc caccgaagcc gcctgcctcg cggtagatac gctcgagctt
     6001 ttcggctacc gtcgcgggcg agccgatcag ccagttgtgc ttcgcgcagt attccggcgt
     6061 cacatcggaa tccgcgacgt taggatcgtg cttcaggtat tcgaggaagc cgaaattccc
     6121 aaggagttgc aggaaatatt cgcgcatcat gcggcccatc atgccgccgg ctgacaggcg
     6181 catggcttct tcatccgtgt cggcgacgaa cacttcgcgc accaggcgcc agtcggcgcg
     6241 tttgggtgtg cggccggtgc gcgcggcacc gatctccacg gcttcccagt ggctcgcgac
     6301 ataggccgga ttgaggttca ggctgagcgg caggaagcct ttctcgccgg caagcttcag
     6361 cgtgtccgac tgcttcgaga agccggcgac gccgatgggc gggtgcggct tttgcagggg
     6421 atagatgtgc ggcttcagga agccgaacat cggctcgggc tttgccacct gccagaactg
     6481 gcccttgtag tcgaacgtcg gttcgtcgct ccacaggcgg aggatgatgt cgagtgcctc
     6541 gcgggtcatc tcgcggttga cgccggcagt gccgtccacc tggaacatgg cccagtcggt
     6601 cggcaggccg gacgcggcga caccgaagtt cagccggcct tccgacagat ggtcgagcat
     6661 tgccacgcgc gcggcgagtt cggcgggatg gtggtagggg agtaggaagc cgccggggcc
     6721 gatacgcagt cgcttcgttt gggtcagcgc ctgggcgatc agcagatcag gcgaggggtg
     6781 cggttcccag ggcgcggtgt agtgctcacc gatccaggct tccttgtagc cgagctcgtc
     6841 gagccagcgc agggtttgca ggtcccagtc gtggccagcc ttcaggccgc gctcgggcgg
     6901 gtgggagggc atggtgaaat agccgaactc catggcgaga cctccttgtt ggttgaagca
     6961 agcctacacc cggacgcgtc gcggttgcca tgtggggtcg gtggcaacgt attggcgccg
     7021 atttggcgcc ggtgccgcgg cgatggccgt tacgatttcg atcgcgaaca ttctcaatga
     7081 ccgggcacga cgcgttgctg cgccggtagt gggtcagcct ggacaaaaga ctgctgagga
     7141 tgcacggggc gttggcgtgc atgcacccat gccgatgccc taggcaacgc cgcgctcgcg
     7201 aagccgaacg atcgcctccg tcgagtagcc aagttctccc agtacctcgg cggtgtgctc
     7261 gcccaacgcg ggggcagggc gccgcactgg cagcggtgcg ccatcgacgt gcagcgcggg
     7321 acccagcatc gacacatcgc cgtggcctgg atgcgccacc gtcacgaccg atcgctgatc
     7381 aatcacctgc ggatcggcga atacctggtc gagctgcatc accggggagc agggcacacc
     7441 ggcctcgttc agcacgcgga tccagtgctc gctggtttcg cgtgtcgtgc gcgcctcgat
     7501 ctcggcgttg atggcggcac ggttcgtggc gcgctgggtg ttggtggcga attccgggcg
     7561 ttcgcgcagg gattcggcat cgagcgcgcg caacagacgt agatacatcg cctcgccagc
     7621 cggggcgatg gcgatctcgc cgtcggcggt gcggaacagg ccatagggcg cggcgatcgg
     7681 atggtcgttg ccggtgcgct tcggcaggtt gccggtcgcg aggtaatctg tggcgaggaa
     7741 gcccaggaag ctgatcaggc cgcccgtgag gctgaccgat gcggttccac cctgacgcgt
     7801 gcgcccctca cgcagcagac cggcgacgat gccaagcgcc gcatagaggc cggcgatcag
     7861 gtcgctgacc ggaggcccgg cgcgcagcgg cgggtgatcg gcggcaccgt tgacgctcat
     7921 gaagccgctc atcgcctggg cgatgaagtc gaagctgggt cgatcgcgat aggggccggt
     7981 ggtgccgaag ccggtgacgc tgcctcgtac cagacgtggg ttcagttcac gcaggcgttc
     8041 gtcggtgaag cccatcgccg acaggacgcc gggacgatag ttgtccatca gcacgtcgga
     8101 ttgccggatc aggccggcca ggatgtcgcg gccttcatcc gagcggaggt tgagcgtgat
     8161 cgagcgcttg ttgcgattgt aattggcgaa gtaccaggag aggccatcgc ggccggcacc
     8221 ctggtggcgg atcgtgtcgc cttcgcctgg ggtttcaacc ttaatgatgt cggcgccgag
     8281 atcgccgagc agcatggtgc agaacgggcc cgacagcacg cgggtcagat cgataatgcg
     8341 aatgccggcg agcggtaatt catcaggcat agaatggtct cacatgtccg ctcatgaact
     8401 ctgcgtaacg tgactgtctt gccgatacgg cattttcaca ctgcagtgca agaaaaccag
     8461 ggaacaggag atccaagatt catgacaaat gtcataagcc gacttgcgcc gggcctggcc
     8521 gccgccgggg cgctggcctg ctggcatcga cagctccggt gtgcgccgct gatgacggtc
     8581 cgatcaagat tggcgtcgtc agcgaggaag ccgccatcgt aggacaggca atcagccagg
     8641 gcgcggaact tgctgtcgaa atcatcacgt atgacaatca ttcatctgct tccggcgcgg
     8701 tgcgcgggta tcagcgcgcc gtgcagcagg accatgtatc ggcggttgta actacattca
     8761 tcagcgaggt ggcgctggcg accgagccgt ggtcggctcg gctgcatatg cccaccatca
     8821 cgcctgcggc ggcgagcgat ctgatcagca agcagctgca cgacgaacaa gatggtgtat
     8881 ccttcattcg gcaaggtaca ggaggcggcg aagtggcgcg ggttcgcagt agctgacgag
     8941 ccgggccttg cgcggactgc aaacactgat cgatgggttc gcgataagct cgctctacgg
     9001 tctcggcgct gtcggcttca cgatcctgct cgcttcgccg aggagatggc gacagcctac
     9061 ctatagatca gccggctgtg gccggtacgc ccggatgccg gccaggccgc tgtggcagca
     9121 ccagcacgca catcacgatg aatgcggcga tcaccgccga ggcgagtggc cggctgagtg
     9181 ccatgccgcc gtcgctgagt ggtttgtcga ggaaatcgcc aacggttgca ccgaggggcc
     9241 gcgtcaggat gaatgccgcc cagaacagca gcacgtgcga tgcgtttgtc catacgtaga
     9301 ggacggcgac gatggccagt ccggccgcaa agaccagcgc tccaccgccg tagccgagtt
     9361 ggccggtgtc agcggcccag tcaccaaggg cggttccaag cgtttgggaa aacgtgattg
     9421 cggcccagta gaaggcttcc acccttgggg tggtcaccgt gtcgaccgag atggtgcgtt
     9481 ccgacaagta ccagagcagc agcactgtca gcaggcatgc cagcagcagc aaagagcctc
     9541 cggtatagcc gatgccgagc gagcgatcgg caaagtccgc catcgtggtg ccgaaggtcg
     9601 tggaggcgac gattgtcgcc caatagagga acgggcggaa ccgttcggca cgtatctgcg
     9661 caaggacgag ggcgacgagc agggcgagga agagggcggt gccggcgaga tagccccagt
     9721 tcaaggtcat ggtgactgaa tcgccaccgg tctcgcccag tgtggtggct gcgatcttga
     9781 tcacccagaa cgtgagtgtg atttccggca ccttgctcca ggcggcgcgc cgcgcgctgt
     9841 cgtcgttcat tccggcatga cctcgagacg ataagcatgg cagatggggc ttcctgccac
     9901 acggtctgca ataggtacgc taaaatcgcc gtgcgctgtc ttacatcgcc gtcatggttc
     9961 gacggcgttg ggggcgtcgg gcggtggcgg tcccgccagg tgccgatagg cggcgcccag
    10021 gccgatcagc accacaccga ggccgaggaa cgacacgacg cgccacaggc cgacgaggct
    10081 ggacatgtcg accaggaaca ccttcgccgc ggcaaggccg acgatgccga gcgcggcgag
    10141 gcgcatatgc ctgatgccgg tgtgaatgcc gatggccatc acaatgacgc cataggcgag
    10201 ccaggcaccg gaccatgccc atagttccgc gtcgccgatg gggatgtcgg cgaagccgat
    10261 cgagccgccg ttgaacaagt ggcgaacttc cagggtgagc caggccagtc ctgccagcag
    10321 actgtatcct tccagcacaa tgcgcagctt tggcacgcct gcgaccgctg gatggcggga
    10381 tgcggcgatg gcgagcaacg ccggcagcag ataggcgggc aggagccagt cgatcagcgg
    10441 ggcgttgcca acgggctcgt tagtgagacc agggttcgcg accaggagaa ggacggcgcc
    10501 gatgagggcg agggcgccct gcaccttcca ggcgatatgc agcatcggac gttgcagccg
    10561 ttcggcgata cgcaacgtgg tgaggcacac gaccccgagc gaactgacgt gcaatgcggc
    10621 ttcggggaag ccgagttcgg gtgtggcgag accggctggc gggccgaatg tgtgccgcac
    10681 ctcgagggcg accaggaccg tgaggaatgc ggcactgccg gcttccagca cgcccaccgt
    10741 ggcatcgtcg cggtcgcggc gaaacagcgc ggcggcaacg gcgaaggcga tgccggggac
    10801 gccgtaggcg atcagcaggt cgttgacgat cggccactgg ccgaagccgt aatccaacac
    10861 gtaccagttc agcagcaggc ggaccaatac aattgtagcg acggcgaggg cgacgcggcg
    10921 cagcgcggga agctcagctt cggcggcgat ccacgcgagt gcggggagga acagcgacac
    10981 ggcgatggtc agccactcgt cgcgcaggaa catgccgcag ccgagcgcga gtgccgcgac
    11041 cgcaccggcc gcgtggatgc cggcgcgctg gcgcgtgttg tcgcgcaagg cgcgggtggt
    11101 ggtgccgatc aggccggcgg tgaggaggat ggcgaaagcg gcccagtcga agcgcggctg
    11161 gaactcggtg acgagtgcgt agcaaacggc gagtgtcaat acggggatgc tggcggtcag
    11221 gctggcccag gcgagctgag tatcggggcg gcgctccaat gcgaggccac tggcggcgaa
    11281 gaagccggac acgagggcgg cggtgaggag cagcgattgt tcagatccgt ctgtgttcca
    11341 ggttgcgagc agggccagga acagcgcagc ggccaggaat ggcagcaggc gcaggcgtgg
    11401 ttcgcgcgac gcgtgccaga cgagcagcgg tgcgagcagc agcacgccga tgcgcgtgat
    11461 cgcggtctgg tcgtcgatcg cgagcgacag gccggcggca ccaagcgcgg cgcacgggat
    11521 gaaggcgagc ttgcggccga tgggatggtt gagtgccgcg gcaggcagaa ggccgaggtt
    11581 cagagctgcg gcggcgggta cgaagagcgc gggtgcccag acatcgctgc cggcaccgcc
    11641 gaggattgcc agtgcaaccc agatcgcgcc ggcgatggtc gttgcccagc cgagccagat
    11701 ccatgccgtg tagcggacaa cggcgagcgc ggcggcggtg acgaacagga ggtagccgta
    11761 gaggcccggg atcgaagggt tctcggtgtt gacgagcgcg ggggtgacga aggcgccgag
    11821 cagtccgatc gccgcaacca cttggccgta gcgcagcgac agggcaatgc cggcgaccga
    11881 ggcggccgcc atcagcacga agcccgcctg tggtggcacc agtccataca tcgcgctggc
    11941 gccgtagctg gcgccgaaca agattgcgac gcctccggcg gcgaggcctg gggaagcgtt
    12001 gtcggcgacc gccgggtgga ggatctcgcg cccgcgcagc cattccgccg cggcgatgag
    12061 ggcgacgccg aggaggccgg cgagcacgca gcgggggccg gggccgagca ggccttgatc
    12121 caccgcgtag cggatcagga aaacaccggc gagcaccagt gccgcggaac caagccagac
    12181 gccccagcgg gcggtcagca gcgtttcgat gtcgggccgc gaagcgcgca ccggctcagg
    12241 tgttgggagc ggtggcggga cgagaggttc gaggggaggg ggctctggcc cggggacagg
    12301 tggcggcggg gggactgcga caggctgctc gggaagtgga acggtttgct ggacggcggc
    12361 ggtgcgcaac tggcccacga gccggcgcag ttcatgggtc tcccgccgaa cgcccagcgc
    12421 gaggaaaaag cctatcacgc ccagcagcca acccgcggca gcgatcagga caaacggcac
    12481 tatgtcgtcc attccgaacc ctatgtcgga catgtgacag gctgtgaccg ttacggcggc
    12541 ctcgtcaaat gattcgagcg gtggggctgc ttgaagcgat ggaatcttcg gcgcgcttat
    12601 tctttcaggc cgtagaatcc gaacgaggcc accttgcggt agccgattcg ctcataaact
    12661 ggggcgccgg ccagggtggc gtgcaggaca gtccgggtca gcccggtcgc cctgccacct
    12721 tcgaacagtg cctttcgcac cgttgcttcc ccatagcctt ttcgccgcgc ctccggtgcg
    12781 gtcgcgacca aggccaggaa caggcagttg ccggtggcaa tcgtcgccgc agcggagacc
    12841 ggcacaccgt cctgtagccc gaggtagctg aacattccgg atttccacag caccgagcca
    12901 gcaagcccgt cccgtccatg ctcgaggggc atggcatagg cacgggagtt cagatcggcg
    12961 aacgccatca gctgctcctc ggtcttgacc cgcacgaaat ccagctgcgg atgatgcggc
    13021 gcggcgatcg gcaggatatc gcccgccatg ccgaaacctg ggaacaggag gctgagcccg
    13081 gcacgttcgg ccgcggctgg cagtcccgcg cgagcattcg catccagcag gtcatcgaac
    13141 agccagatca aaccgggccg gctcttgcga cgcatatagg cggtggcttt cgcgaagcgc
    13201 tcatccaaca ggcggctgcc gcagcccagg tcggtgaagg tgatgcagtt ccagaaggga
    13261 aatttactgt tggcccatcg gatcgccatg ccggggcgtt ccgatacgtc gccgacgcca
    13321 cggtcgagca cgaatgtccg ccaggtatcc gagagttgct gaaccgattc gatggcttcc
    13381 ggcgaaatgc tgcccatgat gttctccaag gtggcggata ttgacgtgtg ccgcctgtca
    13441 ctggcggcgc tggtcaggtg tggagcggag ccccgaccat gcggcatccg tagaccgcgg
    13501 cgacggtttt gataccgtcg cgatcgaccg cgggcagctg gcggaaaaat ccctcgatgc
    13561 cgccgggcga aaagatggcg agcatccgtg caggcgcatc cgacaggttg gctcacgcgt
    13621 gcggaacgcc tctcggcacg gccagtgtca tcccggcggt gacatcctcg ctgcgttctt
    13681 cgcagatgat gcgcacgacg ccttccagga agatgaaatg ctcatcttcg ttctgatgga
    13741 cgtggagagg tggaccggat cgtggtccag cctcttcctc cagcatggtg aagctgccac
    13801 gcgtgatgtc tgatgcgacg cggatcgcca cccgcgcgcc tggcagccat tgcagccatt
    13861 caatgggtac acgattggca tcccgtggag cggtggtaac ggccactatg gtcaatttga
    13921 tcgccttccg actttgagcg aggccacgct cgcggcgggg cgctcgttga cgatccaatc
    13981 tgactgctac gcggccgtta cgcgtcggca ggaaaggctt acaatcgtaa cttataccaa
    14041 tgctcgatga cggcgactga ctgtcacagg cacgaagatg tggatgccgg gccttccccg
    14101 gcataacggg agcatcgacc ggtctcgctc aataagatgt gggattactg cttcggctgg
    14161 tcgggcagga gcagctccag cagggcaggg cgcaggcggc tgagttcgtc caggtggttg
    14221 gcggagagtt tggccagccg ttcttcggcg gtcggggtga ggttcaggcg gacgcgccga
    14281 cgatcctcgg ggtcgtgttc gcgcgtgatc aatccggcct cggcgaggcg gtccgcaagt
    14341 tcaaccgcac tgtggtggcg gatgcgcagc cgttcggcaa ggtagccgac gctggggatg
    14401 gcgccttccc gtgcgccctt gatggcgagc agcgcctggt gttggcgcgg cttgaggccg
    14461 gcgcgcgcgg cggcggcttc actgaactcc aggaagcagc ggagctggta gcggaactcc
    14521 gacagcaatc ggtagtcctg ctgggtcagc cggcgtgtca cacgcgttcg cggggttgca
    14581 cagaatatat cgtgatgcga tgtatgtggg acctccaggg agaatataaa gacgtgactg
    14641 acaccgcact gcccggcgcg ggcaacggcg ttgccgacca tgccacgcgc gcgggcattc
    14701 gccatctggg cgacttcacc accagcccgc gcgtgctgct gatcgcggcg atcgccttag
    14761 tggttggaac gggtggggtg atcggcgggg tgatcctgct gaagctcatc cagctgtgta
    14821 ccaacttggc gtataatggc gtgttcagct tggccgatct gaagcccgca ggatcgccgc
    14881 ttggcgtggc gagcgtgctt gtgccggtga tcggggcact gatcatcggg ttcatggcgc
    14941 gctttggcag cgagaagatc cgtggtcacg gcattccgga ggcgatcgag gcgatcctgc
    15001 tagggcgcag caagcttgac ctgaaggtgg cgatcctgaa gccgctgtcg tcggcgatct
    15061 cgattggcac gggtggcccg ttcggggcgg aggggccgat catcatgacc ggcggtgcga
    15121 tcggctcact gatcgcgcaa ttgctgccgg tgacggatgc cgagcggaag acgttgctgg
    15181 tggcgggcgc ggcggccggc atgacgacgg tgttcggcac gccgatcgcc gcagtgatgc
    15241 tggcggtgga actgctgctg ttcgaatgga cgccgcggag ttttgtgcct gtcgcggtgg
    15301 ccgcgattgt ggcggagctg gagcggacgt ggctggggct ggcttcgccg ctgtttccgt
    15361 tcgcgggtgg gatggcgatc acgctggtag ccgcgctgta ctgggtgctg gtgggactgc
    15421 tgtccggcgc gctgtcgggg ctgctgacgc ggctggttta tctttgggag gacgcgttcc
    15481 tgaagctgcc gatccactgg atgtggtggc cggcgctggg tggcgtcgtg gttgggattg
    15541 gcgggctgat cgatccgcgt gcgctgggtg ttggctacga caatatcgcg cagatgctgc
    15601 aaggcgggat gcctcccgca tcgtcactga tattgctggc agtcaaggcg atcatctggg
    15661 ccgtggcgct ggggtcgggc acgtcgggcg gtgtgttggc gccgctgctg atcatgggcg
    15721 gcggtatggg tgcggcgctg gcccatctgc tgccggttgc cagccccggc ttttgggcgc
    15781 tgctggcgat ggcggcgacg atgggcggca cgatgcgctc gccgctgacc gcgacgttct
    15841 tcgcggtgga aatcaccgga aacacgcatg tgctgctgcc gctgatcacc gcctgcatgg
    15901 cggcacatgt tgtgactgtg ctgataatgc ggcgatcgat cctcaccgag aaggtggcac
    15961 gacgcgggca tcatctgtcg cgcgagtacc gcgtcgatcc gttcgcgctg acgcgcgcgc
    16021 gcgacatcat gacgaccggg gtcgagacgg tgcaggacag catgacgctg caccaggtgg
    16081 cgaggttcat gacgcagccg gacacgcggc atccgagctt cccggtggtg gatgcggcgc
    16141 ggcgtgtgct ggggattgtc gatccgccga cggtgatgcg ttggcgccgg gcgggcgagc
    16201 agcggtgcag gacgctggcg gagctgatgg cccggacgaa ggcggtggtg gcgtaccctg
    16261 atgaatatct cgatgaggtg gttgcgcgga tgacgcaggc gaatgtggcg catattccgg
    16321 tggttgcgcg cgaggacggg cggctggttg ggtatatcgg ttggaaggac ctgatgaatg
    16381 tgcgcgcgcg gctgcaggcg gaagagcgcg agcgggtggt gctgtatcgg gtgaggtaac
    16441 tctcaggtat cggcaatcca acggactttt gcgtggattg ccgcgttgcg gcgcgccgcc
    16501 aggcatccag gatcgcattc gccggcccat gcggtgtggg tcggttcaat ttgtgaggcg
    16561 tgcgcgccag aagcgtttcg ctgcttccag gatggctacg gtgccgatca ccacgagcgc
    16621 caccagcgag aggtcgtccg gatgcaacgg gccgaagcgg aacaactcgc gtcccagcgg
    16681 cgtgccgagc acgaggacca gcagcgccac ggtaatgccc gagatccaaa tgagggcgcg
    16741 atttttctgt ccgaccaggt ctcgcaacga tgcattgaag ttgcgattga ccattatcag
    16801 ccccaggtcc acgagcacca gcgatacaaa tgtcagggcg cgcgcgtcgc cgtcaggcaa
    16861 tccgctgcgg atggtgatcg tgtagaggct tgccaggaat acgaatgcga caattccttg
    16921 caccaggccc caggtgatca gcaccggcga cagcagcggc gttcctgccg cgcggggggg
    16981 ccgacgcatg atgtcggcct cctcagcctc ggcctcgaat accatcgagc ataccggatc
    17041 gatcaccatc tccaggaacg cgatatgcac cggcccgaag atcagcggca ggccggtaag
    17101 gagcggcagc aacgccaatc cggcaatcgg tacgtgcacc gcgatgatat agcccatcgc
    17161 attccgcagg ttgtcgtata tacgccggcc aagccggatc gtctgcacga tcgaaccgaa
    17221 atcatcgtcg agcagcacga gtgaggcggc ctcgcgtgcc acgtcggtgc cgcggccgcc
    17281 catcgcgatg ccaatgtcgg ctgcgcggag cgacggcgca tcgttgacgc cgtcgcccgt
    17341 catggccacc acttcgcctg ccgcgcgcaa ggcccgcacg atccgcagct tctcgtttgg
    17401 catgacgcga gcgaacaccg tggtctgcct ggcagcggcc aggaactcgg cgtcgtccat
    17461 ctgcgcgacc gtctcgccgt ccagcacacg gtcgcagtcg agaccggctc gctgggcgat
    17521 tgcgcgtgcg gtcacggggt aatcgccagt tatcatcacc acgcgaatgc cagctgcccg
    17581 acattgggcg accgcggcgg gcactgaagc gcggagctga tcagcgagac cgagcaggcc
    17641 gactagatga aaatcgtatg cggcagggtc ctcgggcgct cctcctgtgg ttgatgccgc
    17701 cgctacggcg agaacgcgca tcccacccgc ggccaattga tcggcatcgc ggagcaaccg
    17761 gagccgtggt gcgtcatcga gccggcagag ccaggcgatc gcctccggcg cgccctttgc
    17821 ggcgatctga agggcgccgt cggcggagtg ttgccaagcc tggatgaagg cgagccggtc
    17881 cggctgcacg ccgtaaacct tcacgaggtt gccgtctgtc gtgcagctgc cgatcgcctt
    17941 ggcgagcgac tgcgtggcct gatccatcgg atcataggca ttgctggcgc tcgccaatgc
    18001 ggcgatatgc accaaatcat gcagatgcgc aggcacttcg gtgccgcgca gcacggttgg
    18061 cgggcttgct gtcgcctgca acacggctac ggccatgcga ttttcggtca gtgtgccggt
    18121 cttgtcggtg cacagtacgg tcgcctcgcc gagggtctcg atagccgcgg cgcgacgcgt
    18181 caggacacgg gcctgcgaca tgcgccaggc gcccatgacc atgaacacgg tgagtacgag
    18241 agggaactct tcgggcagca tcgacatgcc gagcgcaatg cccgcgagca gcgcgtcgag
    18301 ccatgagccc cgcagcaggc cgaagagcag gatgaccagc aggcagcaga ccaggccgac
    18361 agcggcagcc gcgcggacga gcgctgaagt ctgccggctg agatttgccg gggcggtctc
    18421 gatcgcctgg agtgtctggc cgatccggcc gatctcgctg cgtggtccgg tgctgcgcac
    18481 gatcgcgagg ccttgtccgc ggaccaccag tgtccctgag tagacttggg gactgtcatc
    18541 accgcccgga tgcatcccat cctcagtgcc aagcccgcgg cgcttgcgca ccgggaccga
    18601 ttcgccggtc agcagtgatt catcgatctg gagatcgtgc gcttcgatga gcgtggcgtc
    18661 ggcggcgacg cgatcaccct cggatacagc gagaaggtcg ccgcgcacca cttcgcgtcc
    18721 ggcgatccgt tgccgctggc agtcgcggat gacgagggcg cgggggctgg tgagatcgcg
    18781 caaggcttcc aggacccgtt cgctgcgcgc ttcctgaacg acggcgatgg caacggagag
    18841 ggaggcgaag atcagcagca acagagcttc gaggtggtcg cccagcagca gatagatgac
    18901 gccggcgcct agcagcaggg cgaacatcgg ttcggccagc acatcgtgca ggatgtgggg
    18961 caggttgcgc cggccacgcc tcggcagctc gttgggcccc tcgtggcgca gccgttctgc
    19021 tgcttcgcca gccgttagcc ctgggtgttc ggcctgagcg gcggcgtcca agtgactgtc
    19081 ccgttcaccg catcatcatt ccgtggccgt atgccagccc gggaaggatc tgttgcgcgc
    19141 gttccttctg tgccggagca agtgctgcca ccagtttgat tgctgcctga tgcacggtat
    19201 cggacgcctg ctgccgcgtc tgaaacattg agtccatcag gtttcggcgt tcctgccagg
    19261 aggctgtgcc catcgcttcg aacatcgttt ggtggagagc ctgcatctgc tcgccaacgc
    19321 cggtcaccgt gtcggaatag tctttccaaa caggttcctg gcccgcggtg atgccgaggc
    19381 gtgtcttcag gccgtccaga tagctggcgg tgttccagta accgcccatc atgccgggtc
    19441 ccatgccgcc gcccatcatg ccggggccgc gccccactgt tggttgcgca acggcgacaa
    19501 tggcggcgga tccggcgacg atggttccga gcagcagcct gcgtccgagt tcgggtgcca
    19561 tgaggatgcc tctttggctg atcggagcac acatcgccga tctgccgcgg tggcgcattg
    19621 acctgggtca aggtaggccg cggttgcctc attgctcctt aaccttcttt ccggcatcct
    19681 gcctttcgtc ccagccaccc ggcgcgggag agagtggcgc tcgctctcgg gtttgacccg
    19741 agggttgctc cgccgccgaa ggcgcaaccg gcccccggaa acgctcaggc aaaaggaccg
    19801 cggcgggatc agggatctct ggaaagccgg cgggcatgct gcatgccagc cgcaccgacg
    19861 aggtaagggg cgcgcagcgc cctgaatctt tcaggtaccg cgacagaggg gggtcaactc
    19921 cgtcggcacc gattgccggc gggtgaccct tgtggagcgg accattgatc gagaatgcgc
    19981 tgccggacga cccgacgccg aaggctttga agaccacgcc gctggacgcg tggcatcgcg
    20041 cgcggggcgc gcgcatggtg gagttcgccg gctacagcat gccggtgcaa taccagggcg
    20101 tgatcgcgga gcacctgcag tgccgcgcca aggcggcact gttcgatgtc tcgcacatgg
    20161 ggcaggcgac gcttgccgga cccacggcgg ctgcggcgct ggagagcctg gtgccgggcg
    20221 atatcgtcgg tctgaagccg gggcgccagc gctacacgct gctgaccaac gaggcgggcg
    20281 ggatcaagga cgatctgatg gtcgcgaatc tcggtggcga taagcttttt ctcgttgtca
    20341 acgcggcctg caaggacgtg gatttcaccc atatcggcgc ccatctgccg gccggcgtgc
    20401 agcttcaacc tcacgaggat cgcgcgttgc ttgcgctgca ggggccggcc gcggcggctg
    20461 tgctcgcgcg gctcgggcaa gaggctgcgg gacagccgtt tatgggggtg ctgaatgcga
    20521 tgctgggcgg gattccgtgc ctcgtgtcgc ggtcgggcta caccggtgag gatggcttcg
    20581 agatttctgt gccagccgac cgagctgagg cgctggcgga tcggctcgtc gctgatcctg
    20641 aagtcgcgcc ggcgggtctt ggtgcgcgcg attcgctgcg gctggaagcg ggcctgtgcc
    20701 tctatggcaa tgatatcgac gagctgacaa cgccggtgga agccgggctg acctgggtga
    20761 tcgggaagcg tcggcgcaca agctgggatt tcccgggtgc ggccgcgatt cgcgaccagc
    20821 tggagaatgg cgcggtgcgg catcgcgtcg gcatccgtcc cgatggccgc gcacctgccc
    20881 gtgcgcagac cgatatcgtt gccggtgatg gcacagcggc cgggatcatc acgtccggca
    20941 gcttctcgcc ctcgctgaat gcaccgatcg ccatggggta tgtgcgcaag gaccttgtgg
    21001 ccgatggcac cgccctgcat ctgatcgtgc gtggcaagcc actgccggca cgagtcgtgc
    21061 cgctgccgtt cgtcccccac cgctacgccc actgacaaag gagcgtcccg atggccaaac
    21121 ctccctccga catccgctat accaaagaac atgaatggat ccggttggat ggcgatatcg
    21181 ccactgtcgg catcactgat catgcgcagg agcagctcgg cgacgtggtg ttcgtcgaac
    21241 tgccggaaat cggccgcgat gtggcggctg atgaagcatg cgccgtcgtc gaaagcgtga
    21301 aagctgcgtc ggacatctat gcgccgctga ccggcaagat cgccgagacc aacccgacga
    21361 tcatcgacga cccttccctc gtgaacagcg atgcggaagg cgaggggtgg ttcttccgca
    21421 tcgagatcga cgacaccgac gcgttcgagg cgctgatgga tgcagccgcc tacgacgaat
    21481 atctggagac cctcgactga tggatgtttg ggccgagctc gccgaactgg aagcggcgga
    21541 tggatttgtg gcgcggcata tcgggccgtc cgagtccgat atcgctgcga tgctgcatgc
    21601 agttggcgcg gcgacgctcg atgacctcgc ggcgaagacc gtgccgccgg cgatccgcac
    21661 caaccaggcg ctggagctgc cgcctccgat cgatgaggcg gcggtgatgg cggagctgcg
    21721 cgtcctggcc gcgaagaacg gcggggcgaa gtcgctgatt ggcatgggct atcacggcac
    21781 gcatatgcca ccggtgatcc agcggaatgt gctggagaat cctggctggt acaccgctta
    21841 cacgccgtac caggcggaga tcgcacaggg ccggctggag gcgctgctca acttccagac
    21901 gatgatcagc gagctgaccg cgatggagat cgccaacgct tcgctgctcg atgaaggcac
    21961 ggcggcggcc gaggcgatgg cgatggcgca tgccatcagc aagaccaagt ccgacgtgct
    22021 ggcggttgcc acggacctgc atccgcagac gcgcgcggtg ctggcgacgc gggcgaagcc
    22081 catcggtatc acgctggtgg atttcgcgcc gagcgacctc gcggcgatcg cggcggcgca
    22141 gccgtttgcc gtggtgctgc aatatccggg gacgacgggt gcgatacggg aactgtcgcc
    22201 ggagatcggc gcggcgcatg aggtggatgc gctggtgatc gtctgtgccg atccgctcgc
    22261 cctggcactg ctgacgccgc cgggcgagat gggcgccgat atcgtggtgg gttcgtcgca
    22321 gcggttcggt gtgccgatcg ggttcggtgg gccgcacgcc ggatacatg
//
LOCUS       DATGEM010000251         6709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_462936, whole genome shotgun sequence.
ACCESSION   DATGEM010000251 DATGEM010000000
VERSION     DATGEM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462936"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VKQ27_08060"
     CDS             complement(<1..1948)
                     /locus_tag="VKQ27_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY88923.1"
                     /translation="MPRNVAGWLGHLTHTPSDARRLSRRVAGNDTWAIGLVAAAIYTA
                     IVLSGMGTAVVIALAAVDNELDEVVTGALVFCGATATLAMRKIRDVRREMAARELADQ
                     RADAAAALLEDAVESISEGFVLFDADDRLVLCNRRYHELYPRCAKGIVVGAKFEDLLR
                     DGLAVSQYADAAGREEAWLAERLEQHGRANANVEQRDRDGRWTLITERRMRKGGIAGL
                     RIDITALKDTEAALRDSEERLDLAQEIAGIGSWQYDVEAQRYIWSKQMYRLCGLPPES
                     FVPTSAAVAPYVHPEDELMLRKFRDDLRFGIVREPIKARFIKQSGEIRVLRYEGRPIV
                     DSDGVIRRVLGTAQDVTELDMIQRELAQSQKMEVIGHLAGGVAHDFNNVLGVIIGNLE
                     SLRDHLVDDAKSEELCADALAGALHGAELISRLLAFARRQPLRPQNVDVNEMIRSTAA
                     LFDRLMGEQIEMRLDLGLALWRVLIDPVQMEAALANLVSNARDAMQQSGTLTIATRNL
                     PCELLAAEGQADLAGGDYVVTEVSDTGSGIAPDTISRIFEPFFTTKEQGKGSGLGLSM
                     VFGFINQSRGHVAVVSEPGSGATFRLYLPRCPDQQPVSQAEAVAPVAHVASGSRETIL
                     VVEDNERLRRVTVKQLVDLGYRVFE"
     gene            complement(2032..2835)
                     /locus_tag="VKQ27_08065"
     CDS             complement(2032..2835)
                     /locus_tag="VKQ27_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88924.1"
                     /translation="MTSSFLFGLLVRAIVSASVVVTATVVAEASGPFWSGLIVSLPIG
                     AGPAYAMLALQHPADFIAASALGSFSANAACFVFLTVVALLAPRIHWGRALGGGLLAW
                     VVSVVLIRQFDWDAMTATLLNAGVLLGCLAVTRSAGVEPGASASGPIPRRWFELPARA
                     ALVGLMVGSVVTLSRALGPSLTGMAAVFPIAFTSFTFLILPRVGGVVGAAIMLSALRA
                     MPGFGLSLLTLYLTAVPLGAGLALVVALLPSLAWSALLLLERGLRLRTA"
     gene            complement(2898..4229)
                     /gene="der"
                     /locus_tag="VKQ27_08070"
     CDS             complement(2898..4229)
                     /gene="der"
                     /locus_tag="VKQ27_08070"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLY88925.1"
                     /translation="MLPLVVIAGRPNVGKSTLFNRLAGRRLALVADTPGVTRDRKEAE
                     ALLRGRRVRLVDTAGLEEAPPESLAGRLREGAGVATATADLVVFVVDARAGVTPADRH
                     FAQWLRRQGRPVLLVANKAEGRSSQSSILDAFELGLGEPLAVSAEHGEGISDLMAEIA
                     ERLPDEVEEQEQTGRPLKLAIVGRPNAGKSTLLNRLIGEERMLTGPEPGLTRDAVSVV
                     LDDPGGPIELVDTAGLRRRARIEAPLEKLAVGAAIGALKLAEVVVLVVDAVEGVHDQD
                     LQIGQLIEREGRACVVALTKWDAVVDRSATRRAIGERLEMSLAQMRGLPVITLSALTG
                     VGMDRLLPAVRRAHELWNTRIPTAGLNRWFEQALARHPPPLVSGRRLKLRYVTQAKAR
                     PPTFVLFGTRAERLPEDYQRYLVNSLREAFDLPGTPIRIQLRGTKNPYAER"
     gene            complement(4239..5567)
                     /locus_tag="VKQ27_08075"
     CDS             complement(4239..5567)
                     /locus_tag="VKQ27_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLY88926.1"
                     /translation="MNRGAGFGRRMALLAPLALSGCGLWDDWFGEHKTPLPGKREPVA
                     GGRRALRVDEGVGKVVLPQAISNVAWPQAGGNPAHFMGHLAAGERLAEAWSVDIGAGG
                     GYRRRIMAQPVAANGIVYVMDSDAVVSAFDIPSGGRVWRFDTKKDEDDSTNVGGGLAI
                     DQGTVYAVNGLGELVALDAAKGAMKWRSDFHVPARSAPTIVEGRIFLITIEDRLIALT
                     TDNGRQLWDHQATNPTTSVLGSPAPAYTDGLVVAGFGSGELTAMRAESGTVVWTDSLA
                     ASVVGAGLADFSAIRGLPVIAGGRVYAVSMGGLMVANDLPTGRRLWEREVAGEDHPWA
                     AGSWMFIVSLDQRMAAISRDDGRVAWVTDLPRWENPEKQKNPITWYGPLLVGDRLVVA
                     GTSEMALAVSPYTGEILGRQGLSGKASLGPIAAAGTVFVVTDDGRLLALR"
     gene            complement(5572..6240)
                     /locus_tag="VKQ27_08080"
     CDS             complement(5572..6240)
                     /locus_tag="VKQ27_08080"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY88927.1"
                     /translation="MVDIFDEVEEELRAERAQQMLKRYGGLIIAAVIAIVGAAAGWQG
                     WRWYEARRDAAAAVEYLTAMNLADASTSAGTSSANRAAATAAFASVAASAPDGYATLG
                     RLREAALKADSSDLQGAAALWDQVAADQSADTLLRDLAVLLWAQHLLDKADPGVLQAR
                     LKPLAEPDNPWRTLAQEQLALLDLRLGKRDEAKTALTHLAQDITAPSGVRARAAALVG
                     RLGG"
     gene            6427..>6709
                     /locus_tag="VKQ27_08085"
     CDS             6427..>6709
                     /locus_tag="VKQ27_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88928.1"
                     /translation="MQHHWDVEADVVVVGFGAAGAAAAVTAHELGASVVLLEKAPQGE
                     HGGNTRVAGQGYLNTSSVEKAVAYLTALCGPFTVPEEMVRVWAEEMGQNN"
BASE COUNT         1242 a         2394 c         2000 g         1073 t
ORIGIN      
        1 attcaaacac acgatatccc aggtcgacga gctgcttcac cgtcacgcgc cggagccgct
       61 cgttatcttc gaccaccagt atcgtttcgc gggaacctga tgcgacgtgg gcaaccggtg
      121 cgaccgcttc cgcctggctc accggctgct gatccggaca tcgcggcaga tagagcctga
      181 acgtggcgcc agaaccgggt tcgctgacca ccgccacgtg gcctctggac tgattgatga
      241 atccaaaaac catcgacagg ccaagaccgg atcctttgcc ctgctccttg gtggtgaaga
      301 aaggctcgaa gatccggctt atggtatccg gcgcgatgcc gctgccggtg tccgacacct
      361 cggtcaccac atagtcgccg ccggcaaggt ctgcctgtcc ctcggctgcc aacagctcgc
      421 acggcagatt gcgcgtcgca atcgtcagcg ttccactctg ctgcatcgca tcgcgcgcgt
      481 tcgagacgag attggccaat gctgcctcca tttgaaccgg gtcgatcagc acgcgccaca
      541 atgcaaggcc caggtccagt ctcatctcga tctgctcacc catcagccgg tcgaacagag
      601 ccgccgtgga gcggatcatc tcgttgacat cgacgttttg aggccggagt ggctgccgcc
      661 gcgcgaacgc gaggagacga ctgatgagct cagccccgtg cagcgcacca gccagtgcat
      721 ccgcgcacaa ttcctctgac ttcgcgtcgt ctaccagatg atcgcgcaac gattccagat
      781 tgccgatgat gacgccgagc acgttgttga aatcgtgcgc gacgccgccg gcgaggtgtc
      841 cgatcacctc catcttctgc gattgcgcaa gctcccgctg gatcatgtcg agctcagtga
      901 catcctgggc agtgcccagg actcggcgaa tgacaccatc gctatcgacg atggggcggc
      961 cttcatagcg aagtacccgt atctcgccac tctgcttgat gaaccgagcc ttgatcggct
     1021 cacgcacgat gccgaaccgc agatcgtcgc ggaacttgcg cagcatcaat tcgtcttcgg
     1081 gatgaacata gggtgcaacg gcggccgacg tgggaacaaa ggactctggt ggcaggccgc
     1141 acaggcggta catctgcttc gaccagatat agcgctgagc ctcaacgtca tattgccagc
     1201 tgccgatccc tgcgatttct tgcgcgagat caagccgttc ctcgctgtcg cggagcgcgg
     1261 cctcggtatc cttcagcgca gtgatgtcga tgcgaaggcc ggcgatgccg cctttgcgca
     1321 tgcgccgttc ggtgatgagc gtccaacgcc cgtccctatc gcgctgttcc acattggcat
     1381 tggcgcggcc atgctgttcc aaacgctccg cgagccaggc ctcctcacga ccagccgcat
     1441 cggcatactg gctgacggca aggccgtcac gcagcagatc ctcaaacttc gccccgacaa
     1501 caataccctt ggcgcaccgt gggtatagtt cgtgatatcg cctgttgcac agcaccaggc
     1561 gatcatccgc gtcgaacagg acaaagccct cagagatact ctcgacggcg tcctcaagca
     1621 gggcagcggc cgcatcggcg cgctggtccg ccaactcgcg cgcagccatt tcccgacgca
     1681 cgtcacgtat cttgcgcatt gcgagcgtgg ccgtcgcccc gcagaacacc agtgcgccag
     1741 tcacgacctc gtcgagttcg ttatcgacag ctgccaaggc gatgacgacg gccgtcccca
     1801 tcccgctcag cacgatcgcg gtatagatcg ccgcggcgac cagcccgatg gcccaggtgt
     1861 cgttccccgc cacgcgacgc gacaggcgcc gtgcgtcaga cggtgtgtgc gtcaggtggc
     1921 caagccatcc agcgacattg cggggcattc gcatctgttc gagccaaccc gcccggattg
     1981 cggacgttgt acaattacaa cgccggaccc gccacacttt gctgtcgcca gctacgcagt
     2041 ccgcagtcgg agcccgcgct ccagcaacag cagcgccgac catgccagcg acggcaggag
     2101 tgccacgacc agcgccaggc cggcacccaa aggcaccgca gtcaaataca gcgtcaacaa
     2161 ggatagtccg aagcccggca tggccctgag tgcgctcaac atgatcgccg cgccgacaac
     2221 accgccgaca cgtggcagga tcaggaaggt gaagctggtg aatgcgattg gaaacaccgc
     2281 ggccatgccc gtcagcgacg gccccagggc gcggctcagc gtcaccacgc tgcccaccat
     2341 caatcctacc agcgcggcac gagctggcag ttcgaaccag cggcgcggga tcggaccact
     2401 cgctgacgca ccaggctcaa cgccggcgga tcgtgtcacc gccagacagc caaggagaac
     2461 gccggcattg agcagcgtcg ccgtcatcgc atcccagtcg aactggcgga tcagcaccac
     2521 cgaaaccacc catgcgagca gccctccgcc gagcgcccgg ccccagtgga tgcgaggcgc
     2581 cagtagcgcc actacggtca ggaacacgaa acacgcggca ttggcggaga aactgccgag
     2641 cgcgctggca gcgatgaaat ccgccggatg ctgcaaggca agcatcgcgt aggcggggcc
     2701 ggcgccgatt ggcagcgaga cgatcagccc gctccagaac ggcccggagg cttcggccac
     2761 cacggtcgcg gtgacaacca cagaagcgct gacgatggcg cggaccagca gtccaaaaag
     2821 gaacgaggaa gtcatggcgt ggcgcttgat tcctcctccc cgtgcccccc tcccgaaagc
     2881 ggagaggaaa cagccggtca acgctccgcg taggggttct tcgtgccccg caactgtatc
     2941 cggatcggtg ttcccggcag atcgaacgcc tctcgcaggc tgttgacgag ataacgttga
     3001 tagtcttccg gcagccgctc cgcccgcgtg ccgaacagca cgaaggtggg tggccgggcc
     3061 tttgcctgcg tcacgtagcg cagcttcagc cgcctcccgc tgaccagcgg cggaggatgc
     3121 cgagccaatg cctgttcgaa ccagcggttc agtccggcgg tgggaatccg cgtgttccac
     3181 aactcatgag cgcgccgcac cgccggcaac agccggtcca tcccaacacc tgtcagcgcc
     3241 gacagagtta tcaccggcag gccgcgcatt tgcgccaggc tcatctccag ccgctcgccg
     3301 atggcacgcc gcgtcgcgga acgatccacc accgcgtccc acttggtcag ggcaaccacg
     3361 caggcccggc cctcgcgctc gatcaactgg ccaatctgca ggtcctggtc atgcaccccc
     3421 tcgactgcat cgacgacgag caccaccact tcggccagtt tcagagcgcc gatcgccgcg
     3481 ccaacggcca gcttctcgag cggggcctcg atgcgtgccc ggcggcgcag gccggctgta
     3541 tccaccagtt cgatcggtcc tccgggatca tccagcacga cagacacagc atcacgcgtg
     3601 agccccggtt ccggacccgt cagcatccgc tcctcgccga tcaggcggtt cagcaatgtg
     3661 gacttcccgg cgttcggccg cccgacgatc gcgagcttta acggccgccc agtctgctcc
     3721 tgctcttcca cctcatccgg aagccgttcg gcgatctcgg ccatcagatc ggatattccc
     3781 tcgccgtgct cagccgacac ggcaagcggc tcacccaatc caagctcgaa cgcatccagg
     3841 atcgaagact ggctcgaccg cccctcggcc ttgttcgcaa ccagcaacac gggccgcccc
     3901 tgccggcgca gccattgcgc aaaatgccgg tctgccgggg tcactcccgc tcgcgcgtcc
     3961 accacgaaca ccacgaggtc ggcggtagcg gtcgcgactc cggcgccctc gcgcaaccgg
     4021 cccgcaagcg actcaggcgg cgcctcttca aggcccgcgg tgtccaccag gcgcacccgg
     4081 cgccctcgca gcagcgcctc tgcctccttg cggtcgcggg tgacgcctgg cgtatcagcg
     4141 accaacgcca gccgcctgcc tgccagacga ttgaacaacg tgctcttgcc gacgttagga
     4201 cggccggcaa tgaccaccag gggaagcatc ggccatcctc aacgcagggc cagtagcctg
     4261 ccgtcgtcgg ttaccacgaa taccgtgccg gccgccgcga tcgggccgag cgatgccttt
     4321 ccggacagcc cctgccgtcc gaggatctcg ccggtataag gactgactgc cagcgccatt
     4381 tcgctggtcc ccgccacaac cagacggtcg cccaccagaa gcggcccgta ccaggtgatc
     4441 gggttcttct gcttttcggg attttcccac cgtggcaggt cggtgaccca cgcgaccctg
     4501 ccatcgtcac ggctgatcgc ggccatccgc tgatcgagcg aaacgatgaa catccaggag
     4561 ccggcggccc agggatgatc ctcgcccgcg acctcgcgct cccacagccg ccggccggtc
     4621 ggaaggtcgt tggcgaccat cagcccgccc atactgacgg cataaacgcg cccccctgca
     4681 atcaccggca gaccacgaat ggccgagaag tcggccaggc cggcgcccac cacgctggcc
     4741 gccaggctgt ccgtccacac gaccgtgccg ctttccgcgc gcatcgcggt gagctcgcca
     4801 gagccgaatc ctgctaccac aagaccgtcg gtataagccg gggcaggcga accgagtacg
     4861 ctggtcgttg ggttggtggc ctgatggtcc cagagctgcc gtccgttatc ggtggtcagc
     4921 gcgatcagcc ggtcctcgat ggtgatcagg aaaatacggc cctccacaat ggtcggtgcc
     4981 gaacgcgccg gcacgtggaa atcgctgcgc cacttcattg cacccttggc cgcgtcgagc
     5041 gccaccagtt cacccaggcc gttcaccgcg tagaccgtcc cctggtcgat tgcgaggccg
     5101 ccaccaacgt tggtgctgtc gtcctcgtcc ttcttggtat cgaagcgcca cacccggccg
     5161 ccgctcggga tgtcgaaagc cgacacgaca gcatcggaat ccatcacata gacgatgcca
     5221 ttggcggcta ccggctgcgc catgatcctg cgccgataac caccgcccgc accgatatcg
     5281 acactccacg cctcggccag gcgctcgccc gccgccagat gccccatgaa atgcgccgga
     5341 ttgcccccag cctgcggcca cgccacgttg ctgatcgcct gcggcagaac gactttaccc
     5401 actccttcgt ccacccgcag cgcacggcgc ccccccgcaa ccggctcacg cttgccgggc
     5461 agtggggtct tgtgctcgcc gaaccaatcg tcccacagac cgcatccgct cagcgccagc
     5521 ggcgcaagca aggccattcg gcgcccaaag ccggctcccc tgttcatcgc gtcacccgcc
     5581 cagccggccg accaatgcgg cagcacgcgc gcgcacgcca ctcggggccg ttatatcctg
     5641 cgccagatgg gtcagcgcgg tcttcgcctc atcccgcttg ccaagccgta gatccagcag
     5701 cgcaagctgc tcctgcgcga gcgtccgcca tggattgtcg ggttcggcga gcggcttcag
     5761 ccgagcctgc aaaacgccgg gatccgcctt gtcaagcaga tgctgcgccc atagcaaaac
     5821 cgcgagatca cgcagcagcg tatcggcaga ttgatcggcc gccacctggt cccacagtgc
     5881 ggcagcaccc tggaggtcgc tggaatccgc cttcagcgcc gcttcccgca accgccccag
     5941 cgtcgcgtag ccgtccggcg cgctggcggc gacgctggca aaagccgcag tcgccgcggc
     6001 gcgattggcc gaactggtgc ctgccgaagt ggacgcgtcc gccaggttca tggcggtgag
     6061 atattcgacc gcggcagcgg catcccttcg tgcctcatac cagcgccatc cctgccagcc
     6121 tgcggcggcc cctacaatgg cgatcacggc ggcgatgatg aggccgccat agcgcttcag
     6181 catctgctgg gcgcgctcgg cgcggagttc ctcctcgacc tcgtcaaaaa tgtcgaccac
     6241 gtggtctccc gtctggtgct cgcgccaggg aatccaccgt cccgtgggcg gggcggacca
     6301 tagcgatgtg accgctgtgc ggcaaacacc cgcaattgca ccgcggtgca aaacagaaca
     6361 acattcagcc ggctgatcgg ggaccggctc cgccggccga agcacgtcgc ggagcacgaa
     6421 agttcgatgc agcatcactg ggatgtcgag gccgatgtcg tggtcgtcgg gttcggcgcg
     6481 gcgggcgccg cggcggcagt caccgcccac gagttgggcg ccagcgtggt ccttctcgaa
     6541 aaggcgcccc agggcgagca cggcggcaac acgcgcgtcg ccggccaggg ttatctgaac
     6601 acttcgtccg tcgagaaggc agttgcctat ctcaccgcgt tgtgcgggcc gtttaccgtg
     6661 ccggaggaga tggtgcgggt atgggccgag gagatgggcc agaacaatt
//
LOCUS       DATGEM010000252         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_473129, whole genome shotgun sequence.
ACCESSION   DATGEM010000252 DATGEM010000000
VERSION     DATGEM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473129"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="VKQ27_08090"
     CDS             complement(<1..1265)
                     /locus_tag="VKQ27_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88929.1"
                     /translation="MFVEAVSPLTWSAYGPYAWDAGWRDAFCEIGLFTRDEFKPAGQC
                     EIIGCFGGYVYINMSVTRVLAVRIPGMTIEAIDQSLFGDYPDVPPYRADPRDQNAERT
                     AEVSIWLQSLFTTDPKPVTDQDRVWLDALIAKRPDFAKMSDAQLLGYFRSLRAQARRL
                     FKRHVLNTYGGNVLTSVIAQICQAVGAGDFASKVTAAVGDVDSAFQSFELWELSRQVK
                     SSPVVAAAFDQGVEGLLRRLRGSADPGAKNFLVQWDVFIGHWGFIGPSVWEFRSPTYR
                     THPEIPLRMLDRARQAPDSSSPYARSATLIAEREAAIAEIAGRLAGNADVRGQFMAAA
                     RSAGNYLAARERGKMHCTLIIDEARAPLRALGQRLIRRGQLSRWEDVLLVTDEEADAF
                     VANPAAHAALLAERAARLEMLKAKEPPFV"
     gene            complement(1547..>2191)
                     /locus_tag="VKQ27_08095"
     CDS             complement(1547..>2191)
                     /locus_tag="VKQ27_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HLY88930.1"
                     /translation="ILVAAAPQAISSNFFCLLARADIALSVTLTAVSSALAVFLTPLI
                     AGLMFGVLLEHEAGFVLPVLAVMRQVAAGLLLPVGAGMLVRHFAPGFVARHRKHLQSL
                     SLAALGVMLVILLVNQARTIQDNLATIVLAAVLFTAGAAVLGFGVAKAFSWSREDMIT
                     LIAAFPSRSLSIATLIAVNVLGRLEFLSFAIVFFLVQAVLLVPVMVLARSRAGT"
BASE COUNT          428 a          736 c          682 g          345 t
ORIGIN      
        1 aacacgaacg gcggctcctt ggccttgagc atctcgagcc gcgccgcacg ttcggcgagc
       61 agggcggcgt gcgccgccgg gttggcgacg aaggcatcgg cctcttcgtc ggtgaccaag
      121 agcacatcct cccagcgcga caactggccg cgccttatca ggcgctgccc gagcgcgcgc
      181 agcggcgcac gcgcttcgtc gataatgagc gtgcagtgca tcttgccgcg ctcgcgcgcc
      241 gctagatagt ttccggccga acgggccgct gccatgaact ggccccgcac gtcggcattg
      301 ccggccagcc ggcccgcgat ctcggcgatg gccgcctcgc gctccgcgat cagtgtcgcc
      361 gagcgcgcgt acggtgacga tgaatcaggc gcctggcggg cgcggtcaag catgcgcagc
      421 gggatctcgg ggtgggtgcg gtaagtcggc gagcggaatt cccagacact tgggccgatg
      481 aagccccaat ggccaatgaa cacatcccat tgcactagaa agttcttcgc acctggatcg
      541 gcggagccac gcagccggcg gagcagccct tcgactcctt ggtcgaaggc tgcggcgacc
      601 acgggcgacg acttcacctg gcgcgacagt tcccacagct cgaaagactg gaatgccgag
      661 tccacatcgc ctaccgccgc agtaaccttg gaggcgaagt caccggcgcc gaccgcctgg
      721 cagatttgcg ctattacgct ggtcagcacg ttgccgccgt aggtgttgag cacgtgccgc
      781 ttgaagagcc gccgggcttg cgccctgagg gaacggaagt agcccagcag ttgcgcgtcg
      841 gacatcttcg caaagtccgg ccgcttcgcg atcagcgcat cgagccagac ccggtcctgg
      901 tcggtcacgg gcttcgggtc ggtcgtgaac agggactgca gccagatgct gacctcggcc
      961 gtacgttcgg cgttctggtc gcgcgggtca gcgcggtacg gcggcacgtc cggatagtcc
     1021 ccgaacaacg actggtcaat cgcctcgatg gtcatgcccg ggatgcggac cgctagcacg
     1081 cgcgtgaccg acatgttgat atacacgtaa ccgccgaagc agccgatgat ttcgcattgg
     1141 ccggccggct tgaactcgtc gcgcgtgaat aagccgatct cgcagaaagc gtcgcgccag
     1201 cctgcgtccc acgcatacgg gccgtatgcg ctccacgtga gcggcgacac cgcctcgacg
     1261 aacacctcgc caacattacc ccgcgtgtag acggggaagc gcgcgctgcc ctcgtcgtcg
     1321 cagacccaga ccttgttcct cataacttct tgtctcctcg ccgccctgcc tcggtaatcg
     1381 gctacgcgcc gtgaactgcc cccacactgc ggcagcgaag aaggcgggct tgccaggcgg
     1441 cgccggcagt attcgagtct tgccccaatg aatcgcaaat taccccttgg tccatcgatc
     1501 cgccttgatc cagcgcaagc tgatttgaac tgcgggcgcc gccggttcag gttccggcgc
     1561 ggctccgggc caggaccatc accggaacca gcagcaccgc ctgcacgagg aagaagacga
     1621 tggcaaagga caggaattcc agccgcccca gaacattcac tgcaatcagc gtcgcaatgc
     1681 tcagactgcg cgagggaaat gcagcgatca gggtgatcat gtcctcgcgg ctccacgaga
     1741 acgcctttgc gacaccgaag cccagtaccg cggcgccagc ggtgaaaaga accgcagcca
     1801 gcacgatcgt cgcgagattg tcctggatcg tgcgcgcctg gtttaccaac aggatcacca
     1861 gcatcacccc cagcgccgcc aggctcaggc tttgaagatg tttgcgatga cgcgccacga
     1921 atcctggcgc gaaatgacgc accagcatgc cggcgccgac cggcagcagc aggccggcgg
     1981 ctacctgccg catgaccgcg agaaccggca gaacgaagcc tgcttcatgt tccagcagca
     2041 ctccgaacat cagtcctgca atcaatggcg tcaagaacac cgccagcgcg ctcgacaccg
     2101 ctgtcaaggt caccgagagc gcgatatcgg cgcgcgccag caggcagaaa aaattcgacg
     2161 aaatcgcctg gggtgcggcg gcaacgagga t
//
LOCUS       DATGEM010000253         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_566487, whole genome shotgun sequence.
ACCESSION   DATGEM010000253 DATGEM010000000
VERSION     DATGEM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566487"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..564
                     /locus_tag="VKQ27_08100"
     CDS             28..564
                     /locus_tag="VKQ27_08100"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLY88931.1"
                     /translation="MAAAVVYIGTEMAGPGHVRHHGRGELVIEPSKASDAVARALIAA
                     GVPTDISDKVRGELWAKLILNCAYNALSGITQLPYGRLVKGAGMTAVMRDLVDECLAV
                     AKADGVTIPGDIDAAVRKIADTMPGQCSSTAQDLTRGKRSEIDHLNGLIVRRGEALGV
                     ATPANRLLHAMVKLIESK"
     gene            complement(657..1325)
                     /locus_tag="VKQ27_08105"
     CDS             complement(657..1325)
                     /locus_tag="VKQ27_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HLY88932.1"
                     /translation="MSRLGMVLNLDRCIGCWACAVACKMENSIGEGLWWQEIKTIGGA
                     DIDTSAGVFPNVSKHYQPRNCFHCADPPCLPVCPTDAIVQRADGIVEIIYDKCIGCGK
                     CEPACPYDAIEMNHFAPVLPPGLEHGHGAAEVAPRRAHVVEKCTFCSHRVDQGLQPAC
                     VEACPTQVITFGDVDDPHSAVARERAKADAFRINEQSGADPSVWFLPAQAGAKQRRST
                     EEPS"
     gene            complement(1322..>2411)
                     /locus_tag="VKQ27_08110"
     CDS             complement(1322..>2411)
                     /locus_tag="VKQ27_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HLY88933.1"
                     /translation="RLPRMAREKPLPAWARATPVPMIEVTQRELPVRALFCQGDWLNQ
                     RMGDMNKTTEYLSKLEFVVTVDHFWQTTTQWSDIVLPASTFLEATAPVRDAVVTGNSV
                     LLRQKVIDPVGDSRPDADIERDLARRLGLGEWFTETAEEIVRGQVDGARDPAFAGITY
                     ERLVAAGGGLRLDVPRTPNVQYADLCFDTPSGRAELYLEKLADMGEALPVYVEDHEAQ
                     RSHPLASEFPLVLVQAHVRQRAHSTFFNTGWTLEVWPEPTLEMNPDDATARGLASGDL
                     GEAHNHRGHVVARVVCNPDLPPGMCNLGEGWKQQQYIDGNVQMLTNDLINDAQTRVWG
                     HSNIPFYDTRIEVRRAKARDAAKAAAKS"
BASE COUNT          424 a          780 c          775 g          432 t
ORIGIN      
        1 cggctgcgcg tactcctgcg gcaggaagtg gccgcagccg tcgtgtatat cgggaccgag
       61 atggccggcc ctggacacgt cagacaccat ggccgcggcg aactggtcat cgagccgtcg
      121 aaagccagcg atgcggtcgc gcgtgcgttg atcgccgccg gcgtgccgac cgacatttcc
      181 gacaaggtgc gcggtgagtt gtgggcgaag ctgatcctga attgcgcgta taacgcacta
      241 tctgggatta cgcaattgcc ctacggccgg ctcgtaaagg gcgcgggcat gaccgctgtc
      301 atgcgcgacc tggtcgatga atgcctggct gttgcgaagg cggacggggt gacgattcca
      361 ggcgacatcg acgcggcggt gagaaaaatt gctgacacga tgccgggcca gtgttcgtcg
      421 accgcgcagg atctgacgcg cggcaagcgc agcgagatcg atcatctgaa cggcttgatc
      481 gtgcggcgtg gcgaagcgct gggcgtcgcg acgccggcca accggctcct gcatgcgatg
      541 gtcaaactga tcgagagcaa gtagcagatt tgacccgcgc caaaccccga tcgagcacga
      601 cgtccgcgga tctctggccg cacaaagagc gctaataccc ggagcgatcc cggaactcag
      661 gacggctctt ctgtgctgcg ccgctgcttt gcgccggctt gcgccggcag aaaccatacc
      721 gacggatcgg cgccggattg ttcgttgatg cggaaggcat ccgcttttgc gcgctcgcgc
      781 gccacggcgc tgtgcggatc gtcgacgtcg ccgaaggtaa tcacttgggt cgggcaggcc
      841 tcgacacagg cgggctgcag accttgatcc acacggtgac tgcagaacgt gcacttctcg
      901 accacatgcg cgcgccgagg cgccacctcg gctgcaccgt ggccgtgctc gagaccggga
      961 ggcaagacgg gcgcaaagtg attcatttcg atcgcatcgt aggggcaggc cggctcgcat
     1021 ttgccgcagc cgatacattt gtcgtaaatg atctcgacga taccatcggc gcgctgcacg
     1081 atcgcgtccg tcggacacac cggcaggcac ggcggatcgg cacagtgaaa gcagttgcgc
     1141 ggctgataat gcttgctgac attcgggaac acccccgccg aagtatcgat gtccgcgccc
     1201 ccgatggtct tgatttcctg ccaccacagg ccctcgccga tactgttctc catcttgcat
     1261 gcgaccgcgc acgcccagca gccgatgcag cggtccaggt tcagcaccat gcccagccgg
     1321 ctcatgactt cgccgccgcc ttggctgcgt ccctcgcctt ggcgcgcctc acttcgatac
     1381 gcgtatcgta gaatgggatg ttggagtgcc cccacacgcg cgtctgggca tcattgatca
     1441 ggtcgttggt cagcatctgc acatttccgt cgatgtactg ctgctgcttc cagccctcgc
     1501 cgaggttgca catgcccggc ggcagatcag gattgcaaac cacgcgcgcg accacgtgcc
     1561 cgcggtggtt gtgcgcttcg ccgaggtcgc cgctggccag gccgcgcgcg gtagcgtcat
     1621 cgggattcat ctccagggtg ggctcgggcc atacctccag cgtccagccg gtattgaaga
     1681 acgttgaatg cgcgcgctgc cgcacgtgcg cttgcaccag gaccagcgga aattccgacg
     1741 ccagtgggtg cgaacgctgt gcctcgtgat cttccacgta caccggcagc gcctcgccca
     1801 tgtcggcgag cttctccaga tacagctcgg cacgtccgct cggggtgtcg aagcacagat
     1861 cggcatactg cacgttgggc gtgcgcggca cgtcgagccg caaaccaccc ccggcggcga
     1921 ccaatctctc gtaggtgata ccggcaaacg cgggatcgcg cgcgccgtcg acctggccgc
     1981 gcacgatttc ctcggcagtc tcagtgaacc attcgccgag gccgagccgc cgcgcgaggt
     2041 cacgctctat gtcggcgtcg ggacgtgagt cgccgaccgg atcgattacc ttctggcgca
     2101 acaggacgct gttgccggtg accaccgcat cgcggaccgg tgccgtggct tcgaggaacg
     2161 tcgaggccgg cagcacgatg tcggaccatt gcgtggtcgt ttgccagaaa tggtcgacgg
     2221 tcaccacgaa ctcgagctta ctgaggtatt cggtggtctt attcatgtcg cccatgcgct
     2281 gattgagcca gtcgccctgg cagaacagcg cacgcaccgg cagctcgcgc tgcgtgacct
     2341 cgatcatcgg gaccggcgtc gcgcgcgccc aggccggtaa cggcttttca cgcgccatac
     2401 gcggcaggcg a
//
LOCUS       DATGEM010000254         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_225108, whole genome shotgun sequence.
ACCESSION   DATGEM010000254 DATGEM010000000
VERSION     DATGEM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225108"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VKQ27_08115"
     CDS             <1..471
                     /locus_tag="VKQ27_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY88934.1"
                     /translation="AWAKTGADLRVPAPGQAKKVALLGSLDHAIRELVVHTSPTKRSS
                     DFIAHLEQLDRLYGPRPGQPFTPVVLVEDNGPIHTSKPALAARAHWLTVEWLPKYAPE
                     LNDIEPVWRDLKAHHLAHQTFADADALDQAIHHAVANLNDERMPPPLAKLRIFA"
     gene            712..1944
                     /locus_tag="VKQ27_08120"
     CDS             712..1944
                     /locus_tag="VKQ27_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLY88935.1"
                     /translation="MEVLYPHCAGLDVHKETVVACVRHMSDGVVRREVRTFKTTTSDL
                     LALSEWLASEGCTHVAMEATGVYWKPVWHILGDGDFALTLANAAHVKNVPGRKTDVND
                     ATWLADLLAHGLIRASFVPDAATQEQRGLLRTRKQLVRERASHVQRLQRTLEDANIKL
                     DPAISDVTGVSGRAMIQALIDGEADPARLAELAHRRIRAAPEILREALRGRVTKHHRF
                     LLALHLKQIDALDEAIAGIDQEVDAHVEPFRATIAQLTTIPGISELSACVILAEIGHD
                     MSRFPTQRHLISWAGLCPKNDESAGKRRSSRMRKGAPWLKTTLIQCAWAAARRKGSYL
                     QAQFHRLRARRGAKKAIGAVAASMLTAAYHMLKDGTLYHDLGADHFDRRAKSVATKRL
                     IAKLESLGYDVQITPLAA"
     gene            2194..3426
                     /locus_tag="VKQ27_08125"
     CDS             2194..3426
                     /locus_tag="VKQ27_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLY88936.1"
                     /translation="MEVLHPRCAGLDVHKDMVVACLRLLVDGRPRREVRTFKTVTREL
                     LEMTEWLAGHDCTHIVMEATGVYWKPVWHILADGEFELLLANAAHVKNVPGRKTDVND
                     ATWLADLLAHGLVRASFVPDTPTQELRTLLRTRKQLIRERSSHTMRLQKTLEDANIKL
                     DSVITDIVGLSGRRMIEAVIAGETNPLKLADLAHRRIKASPQQLCDALRGRVTRHHRF
                     LLALHLQQIDAINVAIEQIDQEVDAGVAPYRDAIRLLSTMPGIGDLGACVIVSEIGID
                     MSRFATAGHLISWAGLCPRNDESAGKRRSSRLRKGALWLKTTLVQCAWTASRTKASYL
                     HAQFHRLRARRGVKKALCAVAASMLTAAYHMLRDGTEYQDLGADHFDKRAKTAHTNRL
                     VSRLENLGFSVTLTPVAA"
BASE COUNT          662 a         1265 c         1167 g          589 t
ORIGIN      
        1 gcctgggcga agacgggcgc cgacttgcgt gttcccgcac cgggacaagc aaaaaaggtc
       61 gcgctgctgg gctcgctcga tcacgccatc cgcgaactcg tcgtccacac cagcccgacc
      121 aagcgcagca gcgatttcat cgcccatctc gagcaactgg accggctcta tggtccgagg
      181 cccgggcaac ccttcacgcc cgtcgtgctg gtcgaggaca atggcccgat ccataccagc
      241 aagcccgcgc tcgcagcccg cgcacattgg ctcaccgtcg agtggctgcc caaatacgcg
      301 ccggaattga acgacatcga gcccgtctgg cgcgacctca aggcgcatca tctcgcgcac
      361 cagaccttcg ccgacgctga cgcactcgac caagcgatcc accacgccgt cgccaacctc
      421 aatgacgagc gcatgccgcc tccgttggcc aagctgcgaa tctttgctta gttcgccacc
      481 ggcgttgccg acccgcaggg catgaaacga tggggcggcc ggtgtatgct gcgactgcgt
      541 cgctggcggc gagatcggtg tttccttggt gcgcacaagc cctttgctta gttggatccg
      601 ggaggtctga gcacccctga gtgtgccacg agccggcgac ggctccggag cacgttttct
      661 agagttcttc tgacgggcgt cccaggccgg cggcgcgcca ggaggagcga gatggaagtt
      721 ctctacccgc actgcgccgg cctcgacgtg cacaaggaga cggtggtggc gtgtgtgcgc
      781 cacatgtcgg acggcgtggt caggcgcgag gtccgcacgt tcaagacgac gacctccgac
      841 ctgctggcgc tgtcggagtg gcttgcgtcg gagggctgca cgcacgtcgc gatggaggcg
      901 accggcgtct actggaagcc ggtctggcac atcctcggcg acggcgattt tgcgctgacg
      961 ctggccaatg ccgcgcacgt caagaacgtg ccggggcgca agaccgacgt gaacgacgcg
     1021 acgtggctgg ccgatctgct ggcgcacggc ctgatccgcg cgagcttcgt ccccgatgcc
     1081 gcgacgcagg aacagcgcgg cctgctgcgc acccgcaagc agctggtgcg cgagcgggcc
     1141 agtcacgtgc agcggctgca gcggacgctg gaggacgcca acatcaagct cgatccggcg
     1201 atctcggatg tcaccggggt cagcgggcgg gccatgatcc aggccctgat cgacggtgag
     1261 gccgaccccg cccggctcgc cgagctggcc caccggcgga tcagggccgc gcccgaaatc
     1321 ctgcgcgagg cgctgcgcgg ccgggtgacc aaacaccatc gtttcctgct cgccctgcac
     1381 ctcaagcaga tcgacgcgct cgacgaggcc atcgccggga tcgatcagga ggtcgacgcg
     1441 catgtcgaac cctttcgcgc cacgatcgcc cagttgacca ccattccggg catcagcgag
     1501 ctcagcgcct gcgtgatcct cgccgagatc ggccatgaca tgagccgctt cccgacccag
     1561 cggcacctca tctcctgggc cgggctatgc ccgaagaacg acgagagtgc cggcaagcgc
     1621 cgctccagcc ggatgcgcaa gggcgcgccg tggctgaaga ccacgctgat ccaatgcgcc
     1681 tgggcggcgg ctcgcaggaa gggcagttat ctccaggcgc agttccaccg cctgcgcgcc
     1741 cgccgcggcg ccaagaaggc gatcggcgcc gtcgccgctt ccatgctcac cgccgcctat
     1801 cacatgctca aggacggcac cctctaccac gacctcggcg ccgaccactt cgaccgccgc
     1861 gccaaaagcg tcgccaccaa acgcctgatc gcaaagctcg aaagtctcgg ctacgacgtc
     1921 caaatcacac cactcgccgc ataactggcg gtgagtttct ttctagttcg aaccgacaac
     1981 ggtcgacccg ccgcggatga aacgggtggt atgctgggaa ggtcgtcggc acagaggtcg
     2041 gttgtgtcct tggtgcgttt cagcccgttt tctagttgga cctggagtcc ctgagcaccc
     2101 ccgactgcgc caaggacggt ggcgccgccc cggagcgcgc ttgttagagt tcatcaacgg
     2161 gggctccacg ccgacggccg cgaggaggga gccatggaag tactgcatcc gcgttgcgct
     2221 ggtctggacg tgcataagga tatggtggtg gcctgcctac gcctgctggt ggacggccgg
     2281 ccgcggcggg aagttcggac attcaagacg gtcacccgcg aactgctgga gatgacggaa
     2341 tggcttgccg gccatgattg cacccacatc gtgatggagg cgacgggggt gtattggaag
     2401 ccggtctggc acatcctggc cgatggcgag ttcgagctgc tgctggccaa cgccgcgcac
     2461 gtcaagaacg tgccgggccg caagaccgat gtgaacgatg ccacctggtt ggcggatctg
     2521 ctggctcatg gcctggtgcg cgccagcttc gtgccggaca ccccgacgca ggagttgcgc
     2581 acgctcctgc gcacgcgcaa gcagcttatc cgggaacgat ccagccacac catgcggctg
     2641 cagaagaccc tggaagacgc caacatcaag ctggactcgg tgatcaccga catcgtcggc
     2701 ctcagcggcc gacggatgat cgaggcagtg atcgccggcg agaccaatcc gctcaagctg
     2761 gccgacctcg cccatcggcg gatcaaggcg tcgccgcagc aactctgcga cgccctgcgc
     2821 ggccgggtga ccagacatca ccgcttcctg ctcgccctgc atctgcaaca gatcgacgcc
     2881 atcaatgtcg ccatcgagca gatcgaccag gaggtggatg ccggtgtcgc gccgtatcgc
     2941 gatgccatcc gattgctgag caccatgccg gggatcggcg atctcggcgc ctgcgtcatc
     3001 gtctcggaaa tcggcatcga catgagccgc ttcgccaccg ccggtcatct gatctcctgg
     3061 gccgggctgt gcccgcgcaa cgacgagagc gccggcaagc gacgctccag ccgccttcgc
     3121 aagggcgcgc tctggctgaa gaccaccctg gtgcaatgcg cctggacggc gtccaggacg
     3181 aaagccagct acctgcacgc gcaattccac cgcctgcgcg cccggcgtgg ggtcaagaaa
     3241 gctctctgtg ccgtcgccgc ctccatgctg accgccgcct accacatgct gagagacgga
     3301 accgaatacc aggatctggg cgccgaccat ttcgacaaac gcgccaagac agcccacacc
     3361 aaccgcctcg tcagtcgcct cgaaaatctc ggcttctcgg tcaccctcac accggtcgcc
     3421 gcgtgaaggc gcccgtggcc aatcgaccga ggctctggtt tcttattagt ccggaaggca
     3481 gactgcgatt ttggagagga tgccggtcgg cgcgacccga ccggccgaca gtcacctgcc
     3541 gtatgcctgc gcaatctgcc cgaccagttt tgcccagggc atgtccatcg cgtcgtagac
     3601 ggcgctggcc tccggacgcg cgttgtcttt caggatctca accatcaggg tcgcgtagtc
     3661 cgagacgatc acgccggcct gca
//
LOCUS       DATGEM010000255         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1060978, whole genome shotgun sequence.
ACCESSION   DATGEM010000255 DATGEM010000000
VERSION     DATGEM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060978"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VKQ27_08130"
     CDS             complement(<1..205)
                     /locus_tag="VKQ27_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88937.1"
                     /translation="MRQLLAMPPEIELLPDGSYRPRRPPIATRIFAWAVVICVAAAAL
                     AVASFALWFALILIPVVVVAGAIA"
     gene            253..1218
                     /locus_tag="VKQ27_08135"
     CDS             253..1218
                     /locus_tag="VKQ27_08135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88938.1"
                     /translation="MKVGLFLNTQFQPGQPVAAHLPDLIAQTRAARDAGFKSLWFPHH
                     YLTGPLQMLQIVPMMAYLAPHAEGMQVGPNILILPLLNPVQVAEESATLDVLTSGNYV
                     LGVGLGYRETEFTAFNMSLKERAPRLEESIALMRRLWTEEKVTYKGRFYQVEDGSLCL
                     KPVHPGGPPIWMAGTAEAAIKRAARIADAWMIVPSTTLHDLTAQMRIYREALGHRVPD
                     CFPMVRECYVGENHKTALDECRAALQYKYDAYASWGLSGRQTPVLEELARNRFIIGDK
                     VFVKEEISRYREALGIDHFMMRCHWPGLEFDKALGSIRRLGEIFA"
     gene            1215..2573
                     /locus_tag="VKQ27_08140"
     CDS             1215..2573
                     /locus_tag="VKQ27_08140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY88939.1"
                     /translation="MRQMTLVAFLQAQNCTNFVGSWRHPEASPAFMTADYYRHIARVL
                     EAGKFHVGFFDDRLAMPDRYGGDHAHTVANGIRCVKMDPITILTVMGMATERMGLGST
                     CSTTYYEPFHVARVFQTLDLMTNGRAAWNIVTSMNDGEAFNMGHAAHGEHDQRYDRAD
                     EFLEVVMGHWDSWDDDALVIDKKTGTFAHPDKVRRLDHQGEYFRSRGPFTVPRSPQGH
                     PVLIQAGQSGRGRRFAARWAELVFVNYHSLEHARVDYAEFKKQVAATGRDPEKVAVAA
                     GVYTVVGETRAEAEDKVAMIDQLPKEIDQLSLLCEVLNVDLAQKPIDEPWTEEEIQTW
                     TGAQGLRDRVYRVLGKRNPTTRDFMEVTHRGTFRDHPRFVGSPKDVADGMEEWFSTRA
                     CDGYVIAASHVPGGYEEFVRYVVPELQRRGLMHKDYRGTTLRENLGLVRPAIDAWRKV
                     RH"
     gene            2589..3206
                     /locus_tag="VKQ27_08145"
     CDS             2589..3206
                     /locus_tag="VKQ27_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY88940.1"
                     /translation="MKLMYSPGACSVGIHVLLEEIGKPYDAQLVNLREGGQFKPEFTS
                     VNPKSKVPTLVRDDGSVLTEYPAIAYWLARTNPEKKLLPDDVEAQTRALEALDYCVST
                     LHMHGFSRLFRASSYTPNPADEEKVKERGNEMVEKGFAVMDKVLAGKDYLVGSFSIAD
                     SALFYTEFWGAKRMNMKLPPNCAAHLDRMLARPAVQRVMQQEGLA"
     gene            3208..>3631
                     /locus_tag="VKQ27_08150"
     CDS             3208..>3631
                     /locus_tag="VKQ27_08150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLY88941.1"
                     /translation="MHGAFQEATLAPIPFWFLRHGETDWNAQGISQGNVDIPLNATGV
                     AQARTAAQKLKGRGIATIVASPLSRAKVTAEFVGELLGLPVAIDADLREVGFGVQEGQ
                     PMSNWFADWISGAFTPEGAETFAALRARAVAAANRATVQ"
BASE COUNT          689 a          980 c         1309 g          653 t
ORIGIN      
        1 aggcgatcgc gccggccacg accacaaccg ggatcaggat cagcgcgaac cacagcgcga
       61 atgacgcgac ggccagcgcc gccgccgcca cgcagatgac gacagcccac gcgaagatcc
      121 gcgtggcgat cggtggacgc cggggacgat agcttccgtc tggcagcagt tcgatctcgg
      181 gtggcatggc caagagttgg cgcatccgct tcactggttc aaggtggtag ccgaaccgaa
      241 ggagaccgaa acgtgaaagt cggcctgttc cttaatacgc aatttcagcc gggacagcca
      301 gtggcggcgc atttgccgga cctgatcgcg cagacgcggg cggcgcggga tgccgggttc
      361 aagtcgctct ggttcccgca ccattatcta accgggccgc tgcagatgct gcagatcgtg
      421 ccgatgatgg cgtatctggc gccgcacgcg gaaggcatgc aggtggggcc gaacatcctg
      481 atcctgccgt tgctcaaccc ggtgcaggtg gcggaggagt cggccacgct ggatgtactg
      541 accagcggga attacgtgct cggcgtgggg ctgggctacc gcgagacgga attcaccgcg
      601 ttcaacatgt cgctgaagga gcgggcgcct cggctggagg aaagcatcgc gcttatgcgc
      661 cggctgtgga ccgaggagaa ggtgacgtac aagggacggt tctatcaggt ggaggatggc
      721 tcactgtgcc tgaagccagt gcatcccggc gggccgccga tctggatggc ggggacggcg
      781 gaggcggcga tcaagcgggc tgcacggatc gctgacgcgt ggatgatcgt gccgagcaca
      841 acgctgcatg acctaacggc gcagatgcgc atctaccgcg aggcgctggg gcatcgggtg
      901 ccggactgct ttccgatggt acgcgaatgc tatgtgggcg agaaccacaa gaccgcactg
      961 gatgagtgcc gggcagcgtt gcaatacaag tacgatgcgt acgcgtcgtg gggcttgtcg
     1021 gggcggcaga cgccggtgct ggaagagctg gcacgcaacc ggttcatcat cggcgacaag
     1081 gtgttcgtga aggaagagat ttcgcgctac agggaggcgc tgggcattga tcatttcatg
     1141 atgcgctgcc attggccggg gctggagttc gacaaggcat tgggcagcat tcgtcggctg
     1201 ggagagattt tcgcatgaga cagatgacgc tcgttgcctt ccttcaggcg cagaactgta
     1261 cgaatttcgt tgggtcgtgg cgacatccgg aagcgtcgcc ggcgttcatg acagcagatt
     1321 actaccgcca tatcgcgcgg gtgctggaag cggggaagtt ccacgtcggc ttcttcgatg
     1381 atcggttggc gatgccggac cggtacggcg gcgatcatgc gcatacggtg gccaacggca
     1441 tccggtgcgt gaagatggat ccgatcacca tcctgacggt gatggggatg gcgaccgagc
     1501 ggatggggct ggggtcgacg tgctccacca cgtattacga gcctttccat gtggcgcgcg
     1561 tgttccagac gctggacctg atgaccaatg ggcgcgcggc ctggaacatc gtgacctcga
     1621 tgaacgatgg cgaggcattc aacatgggcc acgccgcgca tggcgagcac gaccagcgct
     1681 atgaccgcgc tgacgaattc ctggaagtcg tcatgggtca ctgggattcg tgggacgacg
     1741 atgcgctggt gatcgacaag aagaccggca cgttcgcgca tcccgacaag gtgcggcggc
     1801 tcgaccatca gggcgaatat ttccggtcgc gcgggccgtt cacagtgcct cggtcaccac
     1861 aagggcaccc ggtgctgatc caggcagggc agagcgggcg agggcggagg ttcgccgcac
     1921 gctgggcgga actggtgttc gtgaactacc actcgctgga gcacgcgcgg gtggattatg
     1981 ccgaattcaa gaagcaggtc gccgctaccg ggcgcgatcc ggagaaggtg gctgtagccg
     2041 ctggtgtgta taccgtagtg ggtgagacgc gcgccgaggc ggaagacaag gtagcgatga
     2101 tcgaccagct gccgaaggag atcgaccagc tttcgctgct ctgcgaagtg ctgaacgtcg
     2161 atcttgcgca aaagccgatc gatgagccgt ggaccgagga agagatccag acctggacag
     2221 gcgcgcaggg gctgcgtgat cgggtctatc gcgtactggg caagcgcaac ccaacgacgc
     2281 gtgatttcat ggaggttacg catcggggga cgttccggga tcatccccgg ttcgtcgggt
     2341 cgccgaagga tgtggcggac gggatggagg agtggttctc aacccgcgcc tgcgacggct
     2401 acgtgatagc tgcgtcgcac gtgcccggcg ggtacgagga gttcgtgcgt tacgttgtcc
     2461 ctgaactgca acgccgcgga ctgatgcaca aggactatcg tggcaccaca ttgcgggaga
     2521 accttgggct ggtgcgccct gcgatcgatg cgtggcgtaa ggttcgtcat taggagagga
     2581 ggaagacaat gaagctgatg tattcgcctg gtgcctgttc cgtcggcatc catgtgctgc
     2641 ttgaggagat cggcaagccg tacgacgcgc agttggtgaa cctgcgcgag ggtggtcagt
     2701 tcaagccgga gttcacctcg gtcaatccga agtcgaaggt gccgacgttg gtgcgggatg
     2761 atggctcggt gctgaccgag tatccggcga tcgcctactg gctggcgcgg accaatcccg
     2821 aaaagaaatt gctgcccgat gacgtggaag cgcagacccg ggcgctggag gcgttggatt
     2881 actgcgtcag cacgttgcac atgcatgggt tctcgcggct gttccgtgct tccagctaca
     2941 cgccgaatcc ggcggatgag gagaaagtga aggagcgcgg caacgagatg gtcgagaaag
     3001 gcttcgccgt gatggacaag gtgctggccg ggaaagacta ccttgtcggc tcgttctcca
     3061 tcgctgactc ggcgctgttc tacaccgagt tctggggcgc caagcggatg aacatgaagc
     3121 tgccgccgaa ctgcgccgca catctggacc gaatgttggc gcgtccggcg gtgcagcggg
     3181 tgatgcagca ggaaggtctg gcctgacgtg cacggggcgt tccaggaggc gacgctggcg
     3241 ccgatcccgt tctggtttct gcgccatggc gaaaccgact ggaacgccca gggcatttcg
     3301 cagggcaatg tcgatattcc gctgaatgcg accggtgtgg cccaggcgcg taccgcggct
     3361 cagaagctga aggggcgagg gatcgcgacg atcgtcgcct cgccgctgtc gcgggcgaag
     3421 gtgactgcag agttcgtggg tgagttgctg gggctgccgg tggcgatcga tgctgatttg
     3481 cgtgaggttg gctttggtgt gcaggaaggt cagccgatga gtaactggtt cgctgactgg
     3541 atcagcgggg cattcacgcc ggaaggggcg gagacgtttg cggcgctgcg ggctcgggcg
     3601 gtggcggcgg ccaatcgggc aacggtgcag c
//
LOCUS       DATGEM010000256         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1332625, whole genome shotgun sequence.
ACCESSION   DATGEM010000256 DATGEM010000000
VERSION     DATGEM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332625"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VKQ27_08155"
     CDS             <1..570
                     /locus_tag="VKQ27_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLY88942.1"
                     /translation="SAIRKAMPIPPTEAIVVVGAGGLGLNAIAVLRAMKHQNIVVVDI
                     SPEKREAALKAGAHKVVDGSGEDVTRRILETAGGPVLAVIDLVNGTQTARFAFEALRK
                     GGKLIQVGLFGGELTVPLPLMAIRALTVQGSYVGNPKELRELVKLAQDGALPALPVAT
                     VPQNQANDALMRLRDGKVTGRLVLKSEAA"
     gene            complement(577..732)
                     /locus_tag="VKQ27_08160"
     CDS             complement(577..732)
                     /locus_tag="VKQ27_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88943.1"
                     /translation="MAVIDVAIQPDGRTRVWFDCRAKPRPEDEERIAQVVADAMADMA
                     DDRKLAN"
     gene            850..1869
                     /locus_tag="VKQ27_08165"
     CDS             850..1869
                     /locus_tag="VKQ27_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLY88944.1"
                     /translation="MSQNSAIRRSVVLKQRPDGAPRREDFEVRVDEVPQPGPGQVVTR
                     TIWLSIDPYMRGRLREQQLYAQPVNPGDVMTGESVGEVVASAHPDFVPGDMVVGSRGW
                     QTHSVSAGSQLVKLPRHGAPLSAYLGVLGMPGATAFRGITEICQPKKGETVVVSAASG
                     AVGSVAGQLAKRTGARVVGIAGGAEKCLWVQDSLGFDDCVDHRSMDLRRELTAACPNG
                     IDGYFENVGGAVQAAVFELLNPFARVAMCGMVSQYNEAEFPPGPNLGFVVGKRILIQG
                     FIVSDKPEKLVEWRKLAGPWVADGSLIYREDIVEGLESAPDALSGMLSGRNFGKLLVR
                     VSEEA"
     gene            complement(1969..>2847)
                     /locus_tag="VKQ27_08170"
     CDS             complement(1969..>2847)
                     /locus_tag="VKQ27_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY88945.1"
                     /translation="RAVRRHAILFVLSASATFTFGSAIVKALTADFPVMEIVAFRSTV
                     GFLLMLPAIIRSGGWAALRTRRPSGHVQRTLYGFIGTITSVYGFGVLPLVTVTALGFA
                     MPLILTIVSVPLLGERVGPRRATAVVVGLGGVLVMLRPWQVGAGAMPLGAVAIVMAGV
                     FTWALSMINIRQMGDAGERNVTIVAWYSLGTALLAALGCFGNWVTPTPWQFGALICAG
                     LTSGIAQLLMTEGYRAAETSLVAPFEYGAIIYATVLGVAIWGEWPDIWSLTGVVILIA
                     SGLYVWHREVTLGIRR"
BASE COUNT          538 a          886 c          957 g          466 t
ORIGIN      
        1 tcggcgatcc gcaaggcgat gccgatccca cccactgaag caatcgtggt cgtgggcgcc
       61 ggtgggctcg gactgaacgc gatcgcggtg ctacgcgcga tgaagcacca gaacatcgtg
      121 gtcgttgata tcagccccga aaagcgcgag gccgccttga aggctggggc gcacaaggtg
      181 gtggatggca gcggcgaaga cgtcaccagg cgcatcctgg aaaccgcggg tggccctgtg
      241 ctggcggtga tcgatctcgt gaacggcacg cagacggcgc gcttcgcctt cgaggcgctg
      301 cgcaagggcg gcaagctgat tcaggtgggc ctgttcggcg gcgagctgac cgtgcctctg
      361 ccattgatgg ccatccgcgc gctgaccgtg cagggcagct acgtcggcaa cccgaaagaa
      421 ttgcgcgaac tggtgaagct ggcgcaggac ggcgcgctgc ctgcgctgcc cgtcgccacg
      481 gtgccgcaaa atcaggcgaa cgatgcgttg atgcggttgc gcgatgggaa ggtgacggga
      541 cggctggtac ttaaatcgga agccgcctga gcggcgtcag ttcgccagct ttcgatcgtc
      601 ggccatgtcc gccatcgcat cggcgacaac ctgggcgatg cgctcctcat cctcggggcg
      661 cggcttggcg cggcagtcga accacacccg ggtgcggcca tccggctgaa tcgcaacatc
      721 gatgacggcc ataacgatct cctgggcgca ctgacgcctc attgatggca tcggtgcaat
      781 tgcagtgcag ttgcaccaca ctccgtctct gtggcgaaat tgggaatgat ccgtacggaa
      841 gggcacgaca tgagccaaaa ttccgcgatc cgccggtcgg tggtgctgaa gcagcgccca
      901 gacggtgcac cgcgccggga ggatttcgag gtccgcgtgg atgaggtgcc acagcccggc
      961 cccggccagg tggtcacgcg aacgatctgg ctgtcgatcg acccctatat gcgcggtcgg
     1021 cttcgcgagc agcagctcta cgcccagccg gtgaacccgg gcgacgtgat gacaggcgag
     1081 tccgtgggcg aggtcgtggc gtcagcgcat cctgatttcg tgccaggcga tatggtcgtg
     1141 ggttcgcgtg gctggcaaac gcattccgtg agcgccggca gccaactggt gaagctgccg
     1201 cggcatggcg cgccgctgtc ggcttatcta ggggtgctcg gcatgccagg cgctacagcg
     1261 tttcgcggca tcaccgagat ctgccagcca aagaagggcg agacagtcgt cgtgtccgca
     1321 gcctcaggcg cggttggttc cgttgccggg cagctcgcca aacggactgg cgcgcgggtc
     1381 gtgggtatcg cgggcggcgc ggagaagtgt ctttgggtac aggacagcct gggtttcgat
     1441 gattgcgtcg atcaccgctc gatggactta cggcgtgaac tgacggcggc ctgcccgaat
     1501 ggcatcgatg ggtatttcga gaatgttggc ggcgcggtgc aggcggcggt gtttgagctc
     1561 ctgaacccgt tcgcgcgcgt cgcgatgtgc ggaatggtgt cgcagtacaa cgaggcggag
     1621 ttcccgcccg gacccaacct cggcttcgtt gtcggcaagc gcattctgat ccagggcttc
     1681 attgtctccg acaagcccga aaagctggtc gagtggcgaa aactcgccgg accgtgggtc
     1741 gcggatggaa gcctgatcta ccgcgaggac atcgtggaag ggctggagag cgcgcccgac
     1801 gctttgtcag ggatgctgag cgggcgcaac tttggcaagc ttttggtacg ggtgagcgaa
     1861 gaggcatgat tcctcaatat cattgcgagg agcgttagcg acgaagcaat cccggaggca
     1921 gtggacacgg ccgagcttgc ctcattgctg acgctcctcg cgctgacact accgcctgat
     1981 ccccagcgtc acctctcggt gccagacgta tagccccgat gcgatcagaa tgacgacgcc
     2041 ggtcaggctc cagatatccg gccattcgcc ccagatcgcg acgcccagaa ccgttgcgta
     2101 gatgatagct ccatactcga atggcgctac cagcgaagtc tcagcggcgc gatagccctc
     2161 ggtcatcagc agttgcgcga tgccggacgt caggcctgca cagatcaacg cgccgaactg
     2221 ccacggcgtc ggcgtgaccc agttgccgaa gcaaccaagc gccgcgagca acgctgtgcc
     2281 caggctatac cacgcaacga tggtaacgtt gcgttcgccc gcgtcaccca tctggcggat
     2341 gttgatcatc gacagcgccc acgtgaatac gccggccatg acaattgcca cggcgcctag
     2401 cggcatcgcg ccggcgccca cctgccacgg ccgcagcatc accaacacgc cgcccagccc
     2461 cacaaccact gccgttgccc gacgcggtcc gactcgttcg cccagcaatg gcacggatac
     2521 gatggtcagg atcagcggca tggcgaagcc gagcgcggtg acagtcacca gcggcaacac
     2581 gccgaagccg taaaccgagg tgatcgtgcc gatgaagccg tagagcgtcc gctgcacgtg
     2641 accggacggc cgccgcgtgc gcagcgccgc ccagccgccc gagcgaatga tcgcaggcag
     2701 catgagcagg aagccaacgg tcgagcggaa ggcgacgatc tccatcaccg ggaaatccgc
     2761 cgtcagggcc ttcacgatcg ccgagccgaa cgtgaacgtg gcggaggcgc tcagcacgaa
     2821 caggatagcg tgacgccgca cggcgcg
//
LOCUS       DATGEM010000257         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_992037, whole genome shotgun sequence.
ACCESSION   DATGEM010000257 DATGEM010000000
VERSION     DATGEM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992037"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VKQ27_08175"
     CDS             complement(<1..332)
                     /locus_tag="VKQ27_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88946.1"
                     /translation="MTADNNFQHLEVLKRNRTKRHHSKEIFVEGVRPINLAIENGWVL
                     SSVAVAHSKQLSRWAQEVCDSAERVLRLPDSLMQQLSDKEEPSELIATFRMKETGLHN
                     IEPDSSLLL"
     gene            complement(364..1572)
                     /locus_tag="VKQ27_08180"
     CDS             complement(364..1572)
                     /locus_tag="VKQ27_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88947.1"
                     /translation="MLGLLRRGTFRWMWAGQTVSMLGDGVHGVALAWLVLTLTGSPIT
                     LGAVIVTGTLPRTALMLFGGALTDRLSARTLLLTSNLARCVLAALLAGLVLGGHLQLW
                     ELFVIEFLFGSADGLFIPAISAVVPDLVDDANLVNANALMGTSEQASMLAAPSAGGVL
                     VALVGTWGAFFANAASFLVAAAGAFPARRAARHHENHPHIGAAIAEGVNLIRSDKELR
                     AVFSIAVVAALAGGSVFLIGIPMLARTRFGLGATGLGLVLSAWGLGQLAGALTAARTG
                     LPRRWGYIIIAMSAASALVYAVMGVTSSIWVVIALLIPLGFGAAYSDDVARPVWIQRH
                     SRPGMMGRIFSLLELPYHSIAPIGMLVFGAIATKSLTIAFEGAAAIMLAAAVAYAVSH
                     TARHLSMERS"
     gene            complement(1644..>2253)
                     /locus_tag="VKQ27_08185"
     CDS             complement(1644..>2253)
                     /locus_tag="VKQ27_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88948.1"
                     /translation="LYVEDLHGGGHHSDIYTTTVPKGKGATTLWPMGWHQGRLVLSVW
                     TACTFEPLQSPNAWHVVDAATGIRTASIGDANCIPGAWPSPAGIACAVVNPGQINSQV
                     NVYDWNGALINSVRTQSGANELSPSGDLLSIGYLFGPGSPSTTIIKISDGTAVTVSGH
                     LACQWIDDSHVLAGDAVIAYPSGSVAPIGHGGVCAGRFPGSL"
BASE COUNT          440 a          697 c          730 g          386 t
ORIGIN      
        1 agcagcaggc tgctgtccgg ctcgatgttg tggaggcctg tctctttcat ccggaacgtc
       61 gcgatcagct ccgacggctc ctctttgtcg ctcaactgct gcatcagcga gtccggcagc
      121 ctgagcactc gctcggccga gtcgcacact tcctgcgccc agcgcgacag ctgcttgctg
      181 tgcgcgactg cgaccgagga cagcacccag ccgttttcga tggccagatt gatcggccgc
      241 acgccctcga cgaagatctc cttgctgtgg tgccgcttcg tgcggttgcg cttgagcact
      301 tcgaggtgct gaaagttgtt gtcggcggtc atcacttcga ttactcgggc tgatgccatg
      361 cgttcagctt cgctccattg agaggtgtct tgccgtgtga ctgaccgcgt aggccacggc
      421 tgcggccaac atgattgccg ccgctccctc aaacgcgatg gtcaggctct tggtggcaat
      481 cgcgccaaag acgagcatgc cgatgggagc gatcgagtgg taaggcagtt cgagcaggct
      541 gaatatccgg cccatcatcc cgggccggct gtggcgttga atccagaccg gtcgcgcgac
      601 gtcatccgaa taggcagcgc cgaatccgag tgggatcaga agcgcgatga cgacccatat
      661 cgagctcgtc acgcccatga ccgcatagac gagggcgctg gccgctgaca tggcgatgat
      721 gatgtagccc caccggcgag gcaggccggt gcgggccgcg gttagcgcac cggcgagctg
      781 cccgaggccc caggcggaca ggacgaggcc gagtcccgtg gcgccgaggc cgaatcgcgt
      841 tcgggccagc atggggatgc cgatcaggaa cacgcttccg cccgcgagcg ccgccaccac
      901 cgcgatcgag aacaccgcac gcagctcctt atcggatcgg atgaggttga ctccctcggc
      961 gattgcggcg ccgatgtggg gatggttctc gtggtggcgc gcagcacgcc gggcggggaa
     1021 tgcgcctgcc gccgccacca ggaaggaggc ggcgttggcg aagaacgcgc cccacgtgcc
     1081 gaccagtgcc accagcacac cgccggcgga cggagcggcc agcatcgagg cctgctcgga
     1141 cgtccccatg agggcgtttg cgttgacgag gttcgcgtcg tcgacgaggt cgggcacaac
     1201 cgcgctgatg gcggggatga acagtccgtc agcggagccg aacaggaact cgatgacgaa
     1261 gagctcccag agctgcaggt ggccgccgag aacaagcccc gcgaggagcg cagcgaggac
     1321 gcagcgagcg agattcgacg tcaacaagag cgtccgtgca gagaggcggt cggtcaatgc
     1381 gcctccgaac aacatgagcg ctgtgcgtgg aagggtgccg gtgacgatga ctgcgcccag
     1441 ggtgatcggc gatccagtca gagtgaggac gagccacgcg agcgccacgc cgtggacgcc
     1501 gtcgccgagc atggaaacgg tctgcccagc ccacatccag cgaaaggtcc cgcgccgcag
     1561 cagtccaagc acggggcaag cgtaagttcc gccgaggagg caggatcaat ccaaccttgg
     1621 gtccaccaag ggatcacctt tagctataaa gaacccggaa atctgcccgc gcacactccc
     1681 ccatgtccga tcggtgctac ggagccagac gggtatgcaa tgactgcgtc accagccaat
     1741 acatgagagt catctatcca ttggcaggcg agatggccgg atactgttac cgcagtgcca
     1801 tcactaatct tgatgatggt cgtggatggt gagcccggcc cgaataaata accaatagat
     1861 agcaaatctc cggacggcga cagttcgttc gcgccgctct gtgtccgcac gctattgatt
     1921 agcgcgccgt tccagtcgta gacattgacc tggctattga tttggcctgg attgaccaca
     1981 gcgcaagcga tacctgccgg agaaggccag gcgccgggga tgcagttcgc gtcgccgatg
     2041 gaggctgttc ggatcccagt cgccgcgtcg acgacgtgcc acgcattggg agactgcagg
     2101 ggctcaaacg tgcacgctgt ccagacgctg agtaccaagc ggccttggtg ccatcccatt
     2161 ggccacaatg tagttgcacc cttgcccttt gggaccgtcg ttgtgtagat gtccgaatga
     2221 tgccctccac catgcaggtc ctctacgtaa agc
//
LOCUS       DATGEM010000258         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1160026, whole genome shotgun sequence.
ACCESSION   DATGEM010000258 DATGEM010000000
VERSION     DATGEM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160026"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VKQ27_08190"
     CDS             <1..1680
                     /locus_tag="VKQ27_08190"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY88949.1"
                     /translation="DGFHLLFSGAGVHALQSQLADSVFHVPAEQMRIACPDVGGGFGV
                     KNALYPEWVMMLWAARTLGRPVRWMSERIEDFITTAQGRDNVTRACLALDAGGRFLAL
                     QADTVANLGAYLSSGGPGSSTNAPANAIGSGYDVPAIFMDVQGVFTNTVPIDAYRGAG
                     KPEVNYMMERLIDAAARQHGFDAIELRRRNLVHEFPYRKALGTVIDCGRFAANLDDAV
                     VAADHAGFAARRTATEASKRLRGIGVTCFMETARGAPNEGAELRFEQDDKVSVLVGTQ
                     SNGMGHETAYAQIAADLLGMPMETFRYVQADTAKVRAGNGHGGARSMHMGGAALCKAI
                     DTMLDKGRVIAARLLQAEQVVFADGRFAVQGEPDRSIDLPALARAARDPANMPDGMET
                     GLDTYVWNLLDLITFPNGCHIAEVEVDPETGEVTLERYSAVDDFGTLINPMLTIGQVH
                     GGVAQGIGQAMLEHTVYDPQSGQMLSGSFMDYTLPRAQDLPDFDITLSGVPTKANPLG
                     VKGSGQAGAMAAPQAIIVAVLDALAPLGVTHIDMPATPERVWRAIRSSKHG"
     gene            1673..>2378
                     /locus_tag="VKQ27_08195"
     CDS             1673..>2378
                     /locus_tag="VKQ27_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY88950.1"
                     /translation="MDNEPQHRIAAEDYLDALAAHGIEHLFCNPGTDFAPIVEAFSRA
                     ARSNRKVPRPMVVPHENAALSMAHGYTMVTGKPQAVMLHTNVGTANSINMLIDASRDR
                     IPLLLTSGRTPFTESGPSGARNAHIHWAQEMYDQAGMLREVVKWDYEMKRGDQAATVV
                     DRALELAMSSPKGPVYLSLPREVLGEVAATPAESNLAHRARPRAPIASAADIETLADW
                     IAEARKPLIITGHLGQD"
BASE COUNT          469 a          701 c          786 g          422 t
ORIGIN      
        1 gacggcttcc acctgttgtt ctccggcgca ggtgttcatg ccttgcagtc tcagcttgcg
       61 gacagcgtgt tccacgtgcc cgcggagcaa atgcgaatcg cctgccctga cgtcggtggc
      121 ggcttcggtg tcaagaacgc gctgtacccc gagtgggtga tgatgctgtg ggcagcacgc
      181 actttgggac ggcccgtgcg ctggatgagc gagcggatcg aagatttcat cactacggcg
      241 cagggacgcg ataacgtcac gcgcgcctgc ctggcactgg acgctggcgg ccgcttcctg
      301 gcgttgcagg ctgacaccgt tgcgaatctc ggtgcatatc tctcgtcggg cggccccggc
      361 agttccacca atgctccggc caatgccatc ggcagcggct atgatgtccc ggcgatcttc
      421 atggacgtgc agggcgtgtt caccaacacg gtgccgatcg atgcgtatcg tggcgccggc
      481 aaaccggagg tgaactatat gatggagcgg ctgatcgatg cagccgcccg gcagcatggc
      541 ttcgatgcta tcgaattgcg gcggcgcaat ctggtgcacg aatttcccta tcggaaagct
      601 ctgggcaccg tgatcgattg cggccggttt gcagccaatc tggatgatgc tgtggttgct
      661 gccgatcacg cgggatttgc cgcacgccgg accgccacgg aggcgagcaa gcggctgcgc
      721 ggcattggcg tgacatgctt catggagacc gcgcgcggcg ctccaaatga aggcgcggaa
      781 ttgcggttcg agcaggatga caaagtctcg gtcctggtcg gcacgcaatc caacggcatg
      841 gggcacgaga cagcgtacgc gcagatagct gctgatctgc tcggaatgcc gatggagaca
      901 tttcgctatg tgcaggccga cacagcgaaa gtgcgcgccg gcaacggcca cggcggtgca
      961 cggtcgatgc acatgggagg cgcggcgctg tgcaaggcca tagatacgat gctcgacaag
     1021 ggccgggtca tcgcagcacg gctgttgcag gccgaacagg tggtgttcgc cgatggacgt
     1081 tttgcggtgc agggcgagcc ggaccggtcg atcgatctgc cagccctggc gcgcgccgca
     1141 cgcgatcctg ccaatatgcc cgatggcatg gagactggcc tcgatactta cgtctggaac
     1201 ctgttggatc tgatcacctt ccccaatggc tgccacatcg cagaggttga agtcgatccg
     1261 gaaaccggag aggtcacgct ggagcgttac agcgcggtgg atgatttcgg cacgttgatc
     1321 aatccaatgc tgacgatcgg ccaggtgcat ggtggcgtgg cgcagggtat cggtcaggca
     1381 atgctggagc acacggtcta cgatccgcaa tcaggccaga tgctgagcgg ctcattcatg
     1441 gactatacgc tgccgcgtgc gcaggacctg ccggacttcg acatcaccct gagcggtgtg
     1501 cctaccaagg caaatccatt gggcgtgaag ggatcaggac aggctggtgc gatggcagcg
     1561 cctcaggcca tcatcgtcgc tgtcctggat gcattggcgc cactgggcgt gacgcacatc
     1621 gacatgccgg ccacgccgga acgggtttgg cgcgcgatca ggagcagcaa acatggataa
     1681 tgaaccgcag catcgcatcg cagcggaaga ctatctcgac gcgctcgcag cgcacgggat
     1741 cgagcacctg ttctgcaatc ccggcactga tttcgccccc atcgtagaag ctttttcgcg
     1801 cgcagcgcgg agcaaccgga aggtgccgcg accgatggtg gtaccgcacg agaatgcggc
     1861 gctgtcgatg gcacacggct acacgatggt caccggcaaa ccacaggcgg tgatgctgca
     1921 caccaatgtt ggtacagcca acagtatcaa catgctgata gacgccagcc gtgaccggat
     1981 ccctctgctg ctgacctcgg gccgcacgcc attcaccgag tcgggtccct caggtgcgcg
     2041 aaatgcgcat atccactggg cgcaggagat gtatgaccaa gcgggcatgc tgcgcgaggt
     2101 ggtgaaatgg gactatgaga tgaagcgcgg agaccaggcc gcgaccgtgg tggatcgcgc
     2161 gctggaactg gcaatgtcgt cgccaaaggg ccccgtgtat ctgtcgctgc cacgcgaagt
     2221 actgggcgaa gtggcagcaa cgccagcgga gagcaatctg gcgcatcgtg cgagaccgcg
     2281 cgcaccaatc gcatcggccg ccgatatcga aaccctggcg gactggatcg cagaagcccg
     2341 caagccgctg atcatcacgg gacacctggg ccaggacc
//
LOCUS       DATGEM010000259         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1307141, whole genome shotgun sequence.
ACCESSION   DATGEM010000259 DATGEM010000000
VERSION     DATGEM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307141"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..485)
                     /locus_tag="VKQ27_08200"
     CDS             complement(72..485)
                     /locus_tag="VKQ27_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88951.1"
                     /translation="MADDELSGDGAPSSLVTIAQVIYGLHALSIVIGLTSAITIVGAF
                     VFGFPSIFAVILNYVYRHGTRGTWLESHFRWQIRTFWFAALWIMIGGMVFVTLFIVLV
                     GIPIAWMIWIAAGLWVIYRVARGWLTLQDRKPMVF"
     gene            complement(576..1850)
                     /locus_tag="VKQ27_08205"
     CDS             complement(576..1850)
                     /locus_tag="VKQ27_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HLY88952.1"
                     /translation="MSEPLKKTDDQSFLRKLGPGLITGAADDDPSGIATYSQAGAQFG
                     LNMLWTSLVTFPLMVGIQIVSARIGRVTGEGLAANIRRLFPRWVLLGIVFLLVVANTI
                     NIAADIAAMGEALQLVAGGGEHGHAVIFGVLSLLLQVFIPYQRYVRVLKWLTLSLLAY
                     VAVAFSVHIDWADAIHKSFVPTLSFNPDFVAVVVAVFGTTISPYLFFWQASQEVEELR
                     AGNGTSSLTSTLEDARLHLRRIQIDTYIGMGFSNLIAFFIILSAAVTLHIAGITQIQT
                     SAQAALALRPIAGEFSFLLFSLGIIGTGMLAVPVLAGATAYAVADSFGWRQGLDRKLF
                     EATEFYAIIAIATLGGVLLDFTPIDPIKALFWSAVINGVIAVPIMVVMMLMATRDDVM
                     GPFVIKRRLKFLGWLATVAMAMAVVTMLATAW"
     gene            complement(1966..3963)
                     /locus_tag="VKQ27_08210"
     CDS             complement(1966..3963)
                     /locus_tag="VKQ27_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY88953.1"
                     /translation="MGTFAVMYQLHYSLNNLSLMALTLSVGFVVDDAIVMLENISRHM
                     EMGKTAMQATLEGAREVGFTIVSMTLSLAAVFIPVLFMGGILGKLLHEFAVTIMAAVL
                     VSGFVSLSLTPMLCSRWLRPVAHGSHGRLYLWSERAFDAFRDAYDVTLKWSIRHSGFT
                     MLVYLAVFAATAWLFSAMPKGFLPSTDTGQLLIFTEAAQDISFDAMAAKQRQAAKIVQ
                     SDPNVEAAMAFIGPSSSGSSQTLNLGRIFIRLKPRTERLPADAIIQELRPKLTSIPGM
                     KVYLQNIPPIRIGGKITKSEYQYTLQDADTQELYHWAPILESKMRGLPAYIDVNSDLQ
                     ITNPTVKVQIDRDKASALGVTAEQVEDALYSAFGTRQVSTIYTSSNEYFVILELEPEY
                     QSSPEALSLLYLRSSAGGLVPLSSVARIERGVSPLTVTHQGQLPAVTLSFNLKPGVSL
                     SQAIEQLNGLIAELRIPQTLIGSFQGTAQAFQSSLAGMGMLLIMSIFVIYLILGILYE
                     SYIHPVTILSGLPTAAFGALLTLMLFKLDLNMYGFVGLIMLIGIVKKNAIMMIDFAIE
                     AQRRDGKPAAQAIYEACLIRFRPIMMTTAAALMGTLPIAMGFGAGSEARRPLGLAVVG
                     GLLVSQLLTLYITPVVYLYLERARTWNERRRGRKPAMTAGA"
BASE COUNT          829 a         1174 c         1273 g          688 t
ORIGIN      
        1 ctcggctagc gccgtcgaaa gcagcgccta accgcgtcct ctgcggagag tggttttctc
       61 gaggcagttc cttagaagac catcggcttg cggtcctgca gcgtgagcca gccgcgagca
      121 acgcggtaga tgacccagag gccggccgcg atccagatca tccaggcgat cgggatgccg
      181 accagcacga tgaacaacgt cacgaacacc atcccgccga tcatgatcca tagcgctgca
      241 aaccagaatg tgcggatctg ccagcgaaaa tgggattcga gccaggtgcc gcgggtgccg
      301 tgccgataga catagttgag aatgaccgcg aagatcgacg gaaaaccgaa gacgaaagcg
      361 ccgacgatgg tgattgcgct ggtcaatccg atgacgatgc tcagggcatg cagcccgtag
      421 atgacctgcg cgatggtgac cagcgagctg ggagccccgt cgccggataa ttcgtcgtct
      481 gccatgtcgt tctccaagat aaataaccta caggcttcga ctcacgcctt ccggacgaat
      541 tcgggcggcc cggaaaattc cggcggcgga tcgcttcacc aagcggtcgc cagcatcgtc
      601 acgacagcca ttgccattgc cactgttgcc agccagccga ggaatttcag ccggcgcttg
      661 atcacgaacg gacccatgac gtcatcccgc gtcgccatca gcatcatcac gaccatgatc
      721 gggaccgcga tgacgccgtt gataacggcg ctccagaaca aggccttgat cggatcgatc
      781 ggggtgaagt cgagcagtac gccaccgagc gtcgcgatgg cgatgatcgc ataaaactcc
      841 gtcgcctcga acagcttgcg gtcgaggccc tggcgccagc cgaagctgtc ggccaccgcg
      901 taggccgtgg cgccggccag caccggcacg gccagcatgc ctgtgccgat gatgccgagg
      961 ctgaacagca ggaaggagaa ttccccggcg atcgggcgca atgccagcgc cgcctgcgcc
     1021 gacgtctgga tctgggtgat gcccgcgata tgcagggtta ccgccgcgga cagaatgatg
     1081 aagaaggcga tcaggttgga gaaacccatg ccgatgtaag tatcgatctg gatgcggcgc
     1141 agatgaaggc gggcatcctc cagcgtgctc gtcagcgagg aagtaccgtt gccagcgcgc
     1201 agttcctcga cttcctgcga agcctgccag aagaacaggt aggggctgat cgtggtgccg
     1261 aatacggcga ccacgacggc gacgaaatcc ggattgaacg aaagtgtggg cacaaacgac
     1321 ttgtggatcg catcggccca gtcgatatgc accgaaaacg cgaccgcgac gtaggccagc
     1381 aaggacagcg tcagccattt cagcacgcga acgtaacgtt ggtagggaat gaacacctgc
     1441 agcagcaggc tgaggacacc gaatatgacc gcatgaccgt gctcgccgcc gcctgcaaca
     1501 agctgcagag cctcgcccat tgcggcaatg tccgcggcga tgttgatggt gttggccacg
     1561 accagcagga agacgatgcc cagcaggacc cagcgcggaa acaggcgacg gatattggcg
     1621 gccaagccct cgccggtaac ccgcccgatg cgcgcgctga cgatctggat gccgaccatc
     1681 aacggaaagg tgaccagcga cgtccacagc atgttcaggc cgaactgcgc tccggcctgg
     1741 gaataggtgg cgatgccgct cggatcgtcg tccgcggctc cggtgatcag gccggggccg
     1801 agtttgcgca gaaaggattg gtcgtccgtc tttttcagcg gttcgctcat gtgttctttc
     1861 ggggaggagc acagggcgaa cggaacgggg tcgcgcggcg ccgcggctgt aacgccggga
     1921 ggagggaatc gttgacgctg cgtgcgacgc gaaaacaacc ccccgtcagg caccggcagt
     1981 catcgccggc ttgcggccgc gacggcgttc attccaggtt ctggctctct cgaggtaaag
     2041 atagactacg ggcgtaatgt acaaggtcag cagttgcgac accagcaatc cgccgaccac
     2101 cgcgaggccc agcggccggc gggcttccga tccggctcca aagcccatgg caatcggcag
     2161 tgtgcccatc agcgccgccg ccgtcgtcat catgatcggc cggaagcgga tcaggcaggc
     2221 ctcgtagatc gcctgcgcgg ccggcttgcc gtccctgcgc tgtgcttcga tggcgaagtc
     2281 gatcatcatg atggcgtttt tcttgacgat gccaatcagc atgatcaggc cgacgaagcc
     2341 gtacatattg aggtcgagct tgaacagcat gagggtcagc agcgcgccga aggcggcggt
     2401 cggcaggccg gacaggatcg tgacgggatg gatgtagctt tcgtagagaa tgcccagtat
     2461 tagataaatt acgaaaatcg acatgatcag cagcatgccc atgccggcca gcgacgactg
     2521 aaaggcctgc gccgtgccct gaaagctgcc gatcagcgtc tgcggaatgc gtagctcggc
     2581 aatgagtccg ttgagctgct cgatagcctg gctgagcgaa accccgggtt tcaggttgaa
     2641 agacagcgtt accgccggca attgcccctg gtgggtgacg gtcagcgggc tgacgccgcg
     2701 ctcgatgcgc gctaccgacg atagcggcac gagaccgccc gcactggatc gcaggtacag
     2761 caaggacagt gcttcggggc tggactggta ctccggctcc agctccaaga tgacaaaata
     2821 ttcattgctc gaggtgtaga tggtcgacac ctggcgagtg ccgaaggcac tgtagagcgc
     2881 gtcctcgact tgctcggcgg tcacgcccag tgcggacgcc ttgtcccggt cgatctgcac
     2941 ttttacggtc ggattggtga tctgcaggtc agagttcaca tcgatgtatg caggcaggcc
     3001 gcgcattttc gattccagga tgggcgccca gtgatacagc tcctgggtat cggcgtcctg
     3061 cagcgtgtac tggtactcgc tcttggttat tttgccgccg atgcgtattg gcgggatgtt
     3121 ctgcagatag accttcatgc ccgggatcga ggtgagtttc ggcctcagct cctggatgat
     3181 ggcgtcggcc ggcaggcgct ccgtgcgcgg cttcagacgg atgaaaatcc ggccgaggtt
     3241 cagcgtctga ctcgatccgg aagaggaagg cccgatgaag gccatggcgg cttcgacgtt
     3301 cgggtcggac tgcacgatct ttgcggcctg tcgctgcttc gccgccatcg cgtcgaaaga
     3361 gatgtcctgc gcggcttcgg tgaagatcag caactggccg gtgtcggtgc tgggcaggaa
     3421 ccccttcggc atggcggaga acagccaggc ggtcgcggcg aaaaccgcaa ggtacaccag
     3481 catcgtgaac ccgctgtgcc ggatcgacca tttcagcgtg acgtcatagg cgtcacgaaa
     3541 cgcgtcgaac gcgcgctcgc tccagagata aagcctgccg tggctgccgt gggcgactgg
     3601 acgcagccag cggctgcaca gcatcggcgt cagcgacagc gacacgaagc ccgacaccag
     3661 cacggctgcc atgatggtaa cggcgaactc gtgcagcagt ttgccgagaa tgccgcccat
     3721 gaacaggacc ggaatgaata ccgcggcgag cgacaacgtc atcgacacga tggtgaagcc
     3781 aacttcgcgc gcaccttcca gcgtcgcctg catcgctgtc ttgcccatct ccatgtggcg
     3841 gctgatgttc tccagcatga cgatggcgtc gtcgaccacg aatcccacgg agagcgtgag
     3901 cgccatgagc gagaggttgt tcaggctgta gtggagctgg tacatcacgg cgaaggtgcc
     3961 gatg
//
LOCUS       DATGEM010000260         5101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1213738, whole genome shotgun sequence.
ACCESSION   DATGEM010000260 DATGEM010000000
VERSION     DATGEM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213738"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VKQ27_08215"
     CDS             complement(<1..617)
                     /locus_tag="VKQ27_08215"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HLY88954.1"
                     /translation="MVAVSAGPVTMSQPVPIRRITSADIRAALREGFADFRAMRGDIL
                     FIALIYPLFSLVIAFASLGGVPLVLFFPMAAGLSLMGPLVAVGFYELARRREAGLEAT
                     WTHFLDIRKSHSFGSVLGVAVLMIALFGSWVWVGGAIHDIFLGTVPPPSIGVFLGQLF
                     GTPEGWAMMLVGNLVGFAYAVVALTLTWVTLPMLVDRPLDSWTAIS"
     gene            794..1822
                     /locus_tag="VKQ27_08220"
     CDS             794..1822
                     /locus_tag="VKQ27_08220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLY88955.1"
                     /translation="MGYRVVVAGATGNVGREMLAILAEREFPIDEIAVLASSRSTGDQ
                     VEFGDTGKMLTIKNIEHFDWTGWDMALFAIGSEATKKYAPIAAKAGCTVIDNSSLYRM
                     DPDVPLIVPEVNPEAIDGYRAKNIIANPNCSTAQMVVALKPLHDAATIKRVVVSTYQS
                     VSGAGKAGMDELFEQSRNIFVGDSAEPKKFTKQIAFNVIPHIDVFLDDGFTKEEWKMV
                     VETKKILDPKIKVVATCVRVPVFVGHSEAISIEFENEISAKQAQDILREAPGIMLIDK
                     REDGGYTTPIECVGEFATFVSRVREDPTVENGLVLWCVSDNLRKGAALNAVQIAELLG
                     RRHLKKAA"
     gene            1827..2279
                     /locus_tag="VKQ27_08225"
     CDS             1827..2279
                     /locus_tag="VKQ27_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY88956.1"
                     /translation="MTVEGGCRCGAVRYSLSIDKLPPIYACHCLQCQTWSGSAFSQQA
                     LLPEEAIHVTGPVIFYELTTGDRTSRQRMCGLCHTRIYNTNTRRPGIAVVRAGTLDES
                     HRLDVIAHIWVKRKQPWLTLPEGVPTWPESAPPADFAAMMMGAAALRV"
     gene            2611..2799
                     /locus_tag="VKQ27_08230"
     CDS             2611..2799
                     /locus_tag="VKQ27_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88957.1"
                     /translation="MMNGAIISTNEISPRIRNQLDAGLGRPKSSVPFPIMINITTPVK
                     RSKRGFGAAGIIPAINQP"
     gene            2943..3389
                     /locus_tag="VKQ27_08235"
     CDS             2943..3389
                     /locus_tag="VKQ27_08235"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY88958.1"
                     /translation="MSEIMTGGCQCGRVRYAVPIDSDEAYLCHCNYCKRATGGVSIAF
                     RTVRFADVQWTATPPDWYQSSPIARRPFCSACGTPLGFQFLDGENMDLTVGSFDEPAR
                     FRPTSNFSIETALEAWMDVTHLPGKRLDEHAPAVDRWIKALGKLPD"
     gene            3331..4137
                     /locus_tag="VKQ27_08240"
     CDS             3331..4137
                     /locus_tag="VKQ27_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY88959.1"
                     /translation="MSTRPPSTAGSRPLASSPTELPIHRFKSFDGTELAYREMGEGRP
                     VVLIHGFFSTATVNWIRYGHAAKIAAKGFRVIMPDLRGHGDSARPHDAAAYPRDALAD
                     DGFALIEHLGLIDYDLGGYSLGGRTTMRMLARGATPRRAIIGGMGLDGIVNTGGRGRH
                     FRNILTNLGSFERGTPEWLAEAFLKTVEGDPVALLHVLDTFVDTPREAVAALDLPVLI
                     VAGVQDDDNGSAEALADALPHGTHRTIPGNHMSAVTRPELGDAIAEFLAG"
     gene            4207..>5101
                     /locus_tag="VKQ27_08245"
     CDS             4207..>5101
                     /locus_tag="VKQ27_08245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLY88960.1"
                     /translation="MESATVIFFGLIATSLLLCIALAVAWRRFGREAHAALWALAYGL
                     SAANWTASLLKIWLLPAGSELTLLSVVCSVGSFALIALGFRRRAGLPLRLDAILAAAG
                     VTVIFAGGGVWDSVNTNAHPWLVALFCSAMLVVSTLSLRAQRHNVRRETGGPAFWMLA
                     LFAAYTLGLGVLALVVKPGINNMGYELYRLLFLMGVPTGLFGVGLFAMFLLAADLAEG
                     MRQLAASDPLTGILNRRGFEQAAQPLADECQRRGRPLAVVIADIDRFKTVNDRFGHSV
                     GDAVLQRFVGHMQTVVRGEDLF"
BASE COUNT          899 a         1649 c         1664 g          889 t
ORIGIN      
        1 gagatcgccg tccagctgtc cagcgggcgg tcgaccagca tcggcagcgt cacccaggtc
       61 agcgtcagcg ccacgaccgc gtaggcgaag ccgaccagat tgccgaccaa catcatcgcc
      121 cagccttccg gcgtgccgaa cagctggccc aggaatacgc cgatcgaggg tggcggcacg
      181 gtgccgagga aaatatcgtg aatggcgccg cccacccaca cccaggagcc gaacagcgcg
      241 atcatcagta ccgccacgcc cagcaccgat ccgaacgagt ggctcttgcg gatgtcgagg
      301 aaatgcgtcc acgtcgcctc cagcccggct tcgcgccggc gggcgagctc gtagaagccg
      361 accgcaacca gcggccccat cagcgacagt cccgcggcca tcgggaagaa cagcaccagc
      421 ggcacgcccc ccagcgacgc gaaggcgatc acaaggctga acagtggata gatgagcgcg
      481 atgaagagaa tgtcgccgcg catcgcgcgg aagtctgcga agccttcacg gagcgccgcg
      541 cgaatatcgg ccgatgtgat cctgcggatc ggtaccggtt gcgacatcgt gacggggccg
      601 gcggatactg cgaccattgc tactctcctc cgatgctcta tctgcactcc ctcggtcggg
      661 gacggtcatg ccgtcggcga tccggtgcgg catctttgcc gtaatcttgt tttaccatag
      721 cgcgcgtcgc tggacaaagc gggacgcaac gctatgccgc acgcgcaata tctgaaggtg
      781 aatggagtct gacatgggtt atcgggtcgt cgtcgcgggt gccacgggga acgttggtcg
      841 cgagatgctc gccatcctcg ccgagcgcga gtttcccatt gacgagatcg cggtgctggc
      901 ctcgtcgcgc tcgaccggcg atcaggtcga gttcggcgac accggtaaga tgctcaccat
      961 caaaaacata gagcatttcg actggaccgg ctgggacatg gcgctgttcg ccatcggctc
     1021 cgaggcgacc aagaaatatg cgccgattgc tgccaaggcc ggctgcacgg tgatcgacaa
     1081 ttcgtcgctc taccgcatgg acccggacgt gccgctgatc gtgcccgaag tgaaccccga
     1141 ggcgatcgac ggctatcgcg caaagaacat catcgccaat ccgaactgct cgaccgcgca
     1201 gatggtggtg gcgctgaagc cactgcacga tgcggcgacg atcaagcgcg tggtggtctc
     1261 gacctaccag tcggtttccg gcgcgggcaa ggcgggcatg gacgagctgt tcgagcagag
     1321 ccgcaacatc ttcgtcggtg acagcgccga gcccaagaag ttcaccaagc agatcgcctt
     1381 caacgtgatc ccgcacatcg acgtcttcct cgatgacggc ttcaccaagg aagagtggaa
     1441 gatggtggtc gagaccaaga aaatcctcga tccgaagatc aaggtggtcg ccacctgcgt
     1501 gcgcgtgccg gtgttcgtcg gccattcgga agcgatctcg atcgagttcg agaacgagat
     1561 ctcggccaag caggcgcagg acatcctgcg cgaggcgcct ggcatcatgc tgatcgataa
     1621 gcgcgaggac ggcggctaca ccacgccgat cgagtgcgtc ggcgagttcg ccaccttcgt
     1681 cagccgcgtc cgcgaggatc cgaccgtgga gaacggcctc gtgctgtggt gcgtcagcga
     1741 caatctccgc aagggcgcgg cgctgaacgc ggtgcagatt gccgagctgc tcggccgtcg
     1801 ccacctcaag aaggcggcct gatccgatga ccgtcgaggg cgggtgccgc tgcggcgcgg
     1861 tgcgctactc gctctcgatc gacaagctgc cgcccatcta cgcctgccat tgcctgcaat
     1921 gccagacatg gagcggcagc gctttcagcc agcaggcgct cctgcccgaa gaggcgatcc
     1981 atgtgaccgg cccggtcata ttctatgagc tgaccaccgg ggaccggacc tcgcgccagc
     2041 gcatgtgcgg cctctgtcac acgcgcatct acaacacgaa cacgcggcgg ccggggattg
     2101 cggtggtgcg ggcggggacg ctcgacgaat cccatcggct cgacgtgatc gcgcatatct
     2161 gggtgaagcg gaagcagccc tggctgacgt tgcccgaagg cgtgcccact tggcccgaaa
     2221 gcgcgccgcc agcggatttc gcggcgatga tgatgggggc agccgcgctg cgcgtgtgaa
     2281 gcgcgccacg cccctgcgag cactccgctc ggcctgagcc tgtcgaaggc ctgtgcttac
     2341 tgttttcgaa ggggaagacc gcccttcgac aggctcgggg cgaacggatg tcgggaattg
     2401 gtggaggccg tgtggaaacg gcgttcactg gatattgcag tgagggcgag cgcgacccgt
     2461 tcaggccttc atcgcttcga tccatcccct tccgcccgat ccggggtggc caccccggat
     2521 cgggcatctc atgatgccca aacaggctca aaagtcataa gacaagacga cagcctcggt
     2581 ggaaagcggc cataggcatc gagcaccaga atgatgaatg gtgccatcat cagcactaat
     2641 gaaatcagcc caaggattcg caaccaattg gatgccggcc ttggtcgacc gaagagcagc
     2701 gttccgtttc caatcatgat caacatcaca actcccgtaa aaaggagtaa gcggggattt
     2761 ggtgcggctg gcatcatccc ggctatcaac cagccgtaag agaggacaaa taggatttcg
     2821 tctgacagga tcgtctgacg gtacctccgt gcgggtgccc cggaggcgaa atttcagccg
     2881 ttcttggtgt caactttgtc aacttcgcgt cccgtgagag gccgccgcag gggagggcgt
     2941 cgatgagcga gatcatgacc ggcgggtgcc agtgcggccg cgtgcgctat gccgtaccga
     3001 tcgacagtga cgaggcctat ctctgccact gcaactactg caagcgggcg accggcggcg
     3061 tttcgatcgc cttcaggacc gtccgcttcg ccgacgtgca atggaccgcg acgccgccgg
     3121 actggtacca gtcctcgccc atcgcgcgac gacccttctg ctcggcgtgc ggcacgccgc
     3181 tcggcttcca attcctcgac ggcgagaata tggacctcac cgtcggttcg ttcgacgagc
     3241 cggcccgttt ccgcccgacc tccaacttct cgatcgagac cgcgctggag gcctggatgg
     3301 acgtcaccca cctgccgggc aagcgcttgg atgagcacgc gcccgccgtc gaccgctgga
     3361 tcaaggccct tggcaagctc cccgactgaa cttcccatcc accgcttcaa gagcttcgac
     3421 ggtaccgaac tggcctatcg cgagatgggc gagggtaggc ccgtcgtgct gatccacggc
     3481 ttcttctcga ccgcgacggt caactggatc cgttacggcc atgccgccaa gatcgccgcg
     3541 aagggcttcc gggtgatcat gccggacctg cgcggccacg gcgacagcgc ccgaccgcac
     3601 gacgccgccg cctatccgcg cgatgcgctg gccgacgacg gcttcgcgct gatcgagcat
     3661 ctcggcctca tcgattatga tctgggcggc tattcgctgg gcgggcggac gacgatgcgg
     3721 atgctcgcgc gcggcgccac gccgcgccgc gcgatcatcg gcggcatggg gctcgacggc
     3781 atcgtcaaca ccggcgggcg cggccggcac ttccgtaaca tcctcaccaa cctcggcagc
     3841 ttcgaacgcg gcacgcccga gtggttggcg gaggcctttc tcaagacggt ggagggcgat
     3901 ccggtggcgc tgctccacgt gctcgacacc ttcgtcgaca ccccgcgcga agcggtggcg
     3961 gcgctcgatc tgccggtgct gatcgtggcg ggcgtgcagg acgacgacaa tggctcggcc
     4021 gaggcgctgg cagacgcgct gccgcacggc acgcaccgca cgattccggg taatcatatg
     4081 agcgctgtca cacgccccga actgggcgat gcgattgcgg aattcctcgc cggctaggct
     4141 ttttccgggg cagactgcgc caaaagctta gcgaattctt caccgaaccg gccccatagc
     4201 gggaccatgg aatccgctac cgtcatcttc ttcggcctga tagcgacgag tctgctgctc
     4261 tgcatcgcgc tggctgtggc ctggcggcgt ttcgggcggg aggcgcatgc cgcactgtgg
     4321 gcgctggcct atggtctttc ggccgctaac tggacggcca gcctgctcaa gatctggctg
     4381 ctgccggcgg ggtcggaact gacgctgctg tcggtggtct gctcggtcgg cagcttcgcg
     4441 ctgatcgcgc tggggttccg tcgccgcgcc gggctgccgc tcaggctcga cgcgatcctc
     4501 gcggcggccg gcgtgaccgt catcttcgcg ggcggtggcg tgtgggactc tgtcaacacc
     4561 aacgcgcatc cctggctggt ggcgctgttc tgttcggcga tgctcgtggt ctcgacgctg
     4621 tcgcttcgcg cgcagcggca caatgtccgt cgcgagacgg gaggcccggc gttctggatg
     4681 ctggcgctgt tcgcagccta tacgctgggt ctcggcgtgc tcgccctcgt cgtgaagccc
     4741 ggcatcaaca atatgggcta tgagctctat cgcctgctgt tcctgatggg tgtgccgacc
     4801 gggctgttcg gcgtcgggct gttcgcgatg ttcctgctcg ccgccgatct ggcggagggc
     4861 atgcgccagc tcgccgccag cgacccgctc acgggcattc tcaaccggcg cgggtttgag
     4921 caggccgcgc agcccctggc cgacgagtgt cagcggcgcg ggcgtccgct ggcggtggtc
     4981 atcgccgaca tcgatcggtt caagacggtc aatgaccggt tcggccactc ggtgggcgat
     5041 gcggtgctcc agcgcttcgt cggccatatg cagacggtcg ttcggggcga ggatctgttc
     5101 g
//
LOCUS       DATGEM010000261         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_98558, whole genome shotgun sequence.
ACCESSION   DATGEM010000261 DATGEM010000000
VERSION     DATGEM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98558"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..494
                     /locus_tag="VKQ27_08250"
     CDS             168..494
                     /locus_tag="VKQ27_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLY88961.1"
                     /translation="MLYELRIYECVPGKLPDLLKRFDTITLKLWEKHGIKQAGFWTTV
                     IGESNQTLYYFVTWDSLAEREKKWNAFQSDPDWISARAKTEEHGAIVANVKNFILTPT
                     QFSAVK"
     gene            complement(600..1964)
                     /locus_tag="VKQ27_08255"
     CDS             complement(600..1964)
                     /locus_tag="VKQ27_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY88962.1"
                     /translation="MANAFVNAAGDTTARITDYILAARWSDLPDNVRREALRSFFNIL
                     GCTIGGARHDVVALADKSLAEYVGSPHATVIGRGRKADALHASLINCLSSSIYSYDDT
                     HAEAVVHPSGPVATAALALAERRPAKGADFLLAVALGIEFVCRLSKAISVPPAKGTVA
                     WSQTGVAAGIGAAIAAAKLLQLDAVQLQYAIGIALSQAAGFRVMHATMVSSFMPAQAA
                     QTGLRAAIMAEHGFTASPAALEGRYGFLEVFAEQPDTDALAGGLGTRFEILRNTYKPY
                     PCGIVIHPIIDACLQLRQEHSIDAAQVDSVQINASPGAMALCDRRNPQNELQAHVSLY
                     HWTSAVLLRGTARIAELQDPTVQDPAVIAFQDKVTTKRDDSLKGDAAVVAITLKDGSR
                     HTCRIDHCIGSAANPMNDDQLTRKFTELAEPVIGQARTREMVERGWGTESTVDAGDIA
                     RAAA"
     gene            complement(1977..2771)
                     /locus_tag="VKQ27_08260"
     CDS             complement(1977..2771)
                     /locus_tag="VKQ27_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY88963.1"
                     /translation="MDLNLQGKAALVTGASRGIGASIAQELAREGVQVCLAARDKAKL
                     EEVAASITAISGSNSTAIFPCDLREPGSADAAVATAVSAFGRLDILVNNAGATKRADF
                     FTLTEDDWMDGFALKFHGYVRTSRAAWPHLRERHGCIVNIVGVGSRTGSAEFTIGGSV
                     NVALLNFSKALADIGIKDGVRVNAINPGLIETERFGRNIERVTRLHNFANRDEAIAFL
                     LKSHGGVNRAGRPEEIGWLTAYLASDKADYIQGCIIDIDGGTTHSL"
     gene            complement(2877..>3057)
                     /locus_tag="VKQ27_08265"
     CDS             complement(2877..>3057)
                     /locus_tag="VKQ27_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88964.1"
                     /translation="LGWVMLRGRAEILHDGTEHDHAQDLLRSRYPQYREMALADLPVI
                     ALRVERVTSWGNLTR"
BASE COUNT          499 a          946 c         1053 g          559 t
ORIGIN      
        1 cggcgctggt ggcgtgcgtt ggcgcgctgg tgatgaccgg gcggctgatg cgttcgccgc
       61 tgatgcggcg tgatccggac aatccatggg gcatgaagcg gccgccttga tcgcgtccgc
      121 gccgcgccgc agactggccg catccactcg aaggggaggg acttacgatg ctgtatgagc
      181 tgcgtatcta tgagtgcgtg ccgggcaagc tgcccgatct gctgaagcgc ttcgacacca
      241 tcacgctgaa gctgtgggaa aagcacggca tcaagcaggc cggcttctgg accactgtga
      301 tcggcgagtc gaaccagacg ctgtattact tcgtgacgtg ggactcgctc gccgagcgcg
      361 agaagaagtg gaatgcgttc cagagtgatc cagactggat ttcggcccgg gcgaagaccg
      421 aggagcatgg cgcgatcgtc gccaacgtga agaacttcat cctgacgccg acgcagtttt
      481 ccgcggtgaa gtagcccgcc gaacccgtca attgcgagga ggcgcagccg acgaagcaat
      541 cccgatcgag attgcttcgt cgctccgctc ctcgcaatga cagggggcgt ggcgccctgc
      601 tacgccgccg cgcgcgcgat gtcgccggca tcgacagtag actccgtgcc ccagccgcgt
      661 tccaccattt cccgagtgcg cgcctggccg atcaccggtt cggcaagctc ggtgaatttg
      721 cgggtcaact ggtcatcgtt cattggattg gcggcgctgc cgatacaatg atcgatacgg
      781 caggtgtggc ggctcccgtc cttcagcgtg atcgccacca ccgccgcgtc gcccttcagg
      841 ctgtcgtcgc gtttcgtggt caccttgtcc tggaacgcga tcaccgcagg atcctgcacc
      901 gtggggtcct gcaactcggc gatgcgcgcc gttccgcgca gcagcaccgc cgacgtccag
      961 tgatacaggc tgacatgcgc ctggagctcg ttctgtgggt tgcgccggtc gcagagcgcc
     1021 atcgcgccgg gcgaggcgtt gatctgtacg ctgtccacct gggcggcgtc gatgctgtgc
     1081 tcctggcgga gttgcaggca ggcatcgatg atcggatgga tcacgatgcc gcacggatag
     1141 ggcttgtaag tgttgcgcag gatctcgaag cgcgtgccga ggccgccggc cagcgcatcc
     1201 gtgtccggct gttcggcgaa gacctcgaga aatccgtagc gaccttccag tgcagccggg
     1261 gaggcggtga agccatgctc ggccatgatc gcggcgcgca ggcccgtctg cgcggcctgt
     1321 gccggcatga acgacgacac catggtggcg tgcatgaccc ggaagccggc cgcctgcgac
     1381 agggcgatgc cgatggcgta ttgcagctgc accgcgtcca gctgcagcag cttcgccgcg
     1441 gcgatcgccg cgccgatgcc ggcggcgaca ccggtctgcg accaggcaac ggtgcccttg
     1501 gcgggtggca cggagatcgc cttcgacagt cggcatacga actcgatgcc gagcgcgacg
     1561 gcgagcagga agtcggcgcc cttggcgggg cggcgttcgg ccagcgccag cgctgccgtt
     1621 gcaaccgggc cggaaggatg caccaccgcc tcggcgtgtg tgtcatcgta tgagtatata
     1681 ctggagctga ggcagttgat caggctggcg tgcagcgcgt ctgccttgcg gcctcggccg
     1741 atcaccgtgg catgcggcga gccgacgtat tccgcgagcg atttgtcggc gagtgccacc
     1801 acgtcgtggc gtgcgccgcc gatggtgcag ccaaggatgt tgaagaaaga tcgcagtgcc
     1861 tcgcggcgga cgttgtcggg caggtcggac cagcgcgcgg ccaggatgta gtcggtgatg
     1921 cgcgcggtcg tgtcgccggc ggcgttgacg aatgcgttgg ccatcgcgag gctcctttac
     1981 agcgagtggg tggtgccgcc gtcgatatcg atgatgcagc cctggatgta atcggccttg
     2041 tcggaggcga ggtaggcggt gagccagccg atctcctcgg ggcggccggc gcggttgacg
     2101 ccaccgtgcg acttcaacag gaaagcaatg gcttcgtcgc ggttggcgaa attgtgcagc
     2161 ctggtcacgc gttcgatgtt gcggccaaag cgttccgttt cgatcaggcc tggattgatg
     2221 gcattgaccc gcacgccgtc cttgatgccg atgtcggcga gcgccttgct gaagttcagc
     2281 agtgccacgt tgaccgaccc gccgatggtg aactccgccg aaccggtgcg cgagccgaca
     2341 ccgacgatgt tgacgatgca gccgtgacgt tcgcgcagat gtggccacgc ggcgcgggac
     2401 gtgcgcacgt agccatggaa tttcagggcg aagccgtcca tccagtcatc ttcagtcagg
     2461 gtgaagaaat ccgcgcgctt ggtcgcgccg gcgttgttga ccaggatgtc tagccggccg
     2521 aacgccgata cggctgtagc gacggcggca tccgcgctgc caggttcccg gaggtcacac
     2581 gggaagatcg cggtgctgtt gctgccggag atcgcggtga tgctggcagc gacctcttcc
     2641 agtttggcct tgtcgcgcgc ggcgaggcag acctgtacgc cctcgcgcgc gagctcctgc
     2701 gcgatcgagg caccaatgcc gcgacttgcg ccggtcacga gcgccgcctt gccctgcagg
     2761 ttgaggtcca ttacgtctcc cccctgttcg ttcccttgct ggaaccctgt cattgcgagc
     2821 gaagcgatgc aatcccgccg agatggcctg gcttggattg caatgacagg ggtgctctag
     2881 cgggtcagat tgccccagct tgtcacgcgt tcgacacgca gtgcgatcac cggcaggtcc
     2941 gcgagtgcca tctcgcgata ttgcggatag cgcgagcgca gcaggtcctg ggcgtgatca
     3001 tgctcggtgc catcgtgcag gatctcggca cgtccacgca gcatcaccca gccgagc
//
LOCUS       DATGEM010000262         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_791234, whole genome shotgun sequence.
ACCESSION   DATGEM010000262 DATGEM010000000
VERSION     DATGEM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791234"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VKQ27_08270"
     CDS             complement(<1..1141)
                     /locus_tag="VKQ27_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY88965.1"
                     /translation="MSAAEAALENQKIGALQLRVAALCTLVQICDGYDLNSVAWAVPS
                     LIREWHLPPALFTAAFLWSSVGILVGALSAGPVGDRFGRRPLLIGSLTIFGIASLLTA
                     TAGSLTMLTVWRFFTGIGIGGGFSGAAALTGDYTPHRLRATMIMATFTGAPAGGFIGG
                     QIVALLLAHFDWPIIFLIGGAFPLVLVVALAIWLPESPRFLASRQNLAPHHASILERL
                     NVAPQQGEPLDVARGNPVAMLFRQGYALQTVLLWIIYFCSLMNLFLFAYWMPTVLNLM
                     GMTPPEAVFASSLRDFGAMFAVLYLGLAIDRIGPERALALHYAAGAIAIAVIALVALP
                     YALLLLVSFLAGMLITGSQTGANGTCGKLYPARMRTTGMGWALGIG"
     gene            1262..1945
                     /locus_tag="VKQ27_08275"
     CDS             1262..1945
                     /locus_tag="VKQ27_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HLY88966.1"
                     /translation="MINKIVQSMAEAMAGIKDGSTVLIGGFGAVGQPNALIDGLIEQG
                     ATDLTVAANNAGVGHVGLARLMELGRVRKIICSFPRSSDPVVFESLYRAGKIELEIVP
                     QGTLAERMRAAGAGVPAFFTATGVGTKMGMGKETREFDGRSYVMERSLPGDVALVEAW
                     EADRWGNLTFKESGRNFNPVMAMAGKLTIVQTQHVRELGELEPDHVVTPGIFVDRVLH
                     VPYGDPTGF"
     gene            2003..>2528
                     /locus_tag="VKQ27_08280"
     CDS             2003..>2528
                     /locus_tag="VKQ27_08280"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HLY88967.1"
                     /translation="MDARTPQPGEITDRNLALELVRVTEAAAIAASAWIGLGKKNEAD
                     GAAVEAMRKAFDQVAISGTVVIGEGEMDEAPMLYIGEKVGAGGPPMDIAVDPLEGTTL
                     TAKGGPSALAVVALAEHGNFLHAPDIYMDKIAVGGGLPEGVVDLDAAVGKNLRELARA
                     KRCEVSDLLACILDR"
BASE COUNT          462 a          744 c          918 g          404 t
ORIGIN      
        1 tgccgatccc gagtgcccat cccataccgg ttgtgcgcat gcgcgcggga tagagtttcc
       61 cgcaggtgcc gttggcgccg gtctggctgc cggtgatcaa catgccggcg aggaagctca
      121 ccagcagcag cagtgcatag ggcagggcaa ccagcgcgat gaccgcgatg gcgatggcgc
      181 cggcggcgta gtgtagagcc agcgcgcgtt ccggtccgat gcggtcgatc gccaggccca
      241 gatacaacac ggcgaacatc gcgccgaagt cgcgcaggct ggaggcgaac acggcctcgg
      301 gcggcgtcat gcccatgagg ttcagcaccg tcggcatcca gtaggcgaac aggaacaggt
      361 tcatcaggct gcagaagtat atgatccata gcagcacggt ctgcagcgcg tagccttggc
      421 ggaacagcat cgcgacggga ttgccgcgcg cgacgtcaag cggttcgcct tgttgcggtg
      481 cgacgttgag gcgctcaaga atgctggcgt gatgcggtgc caggttctgc ctgcttgcga
      541 ggaagcgtgg cgactcgggt agccagattg ccagcgccac aaccagaaca agtgggaatg
      601 ctccgccgat caggaaaatg atcggccagt cgaaatgcgc gagcagcagt gcgacgatct
      661 ggccgccgat gaagccgccg gccggcgcgc cggtgaaggt cgccatgatc atcgtggcgc
      721 gcaggcgatg cggcgtatag tcgccggtca atgcggccgc gcccgagaag ccaccgccga
      781 tgccgatgcc ggtaaagaag cgccacacgg tcagcatggt gagcgatccg gccgtcgcgg
      841 tgagcaacga ggcgatgccg aagatcgtga ggctgccgat cagcagcggg cggcgaccga
      901 agcggtcacc gacgggaccg gcggagagcg cgcccaccag gattccgacg ctggaccaga
      961 ggaaagcggc ggtgaacagt gccggtggca gatgccattc gcggatcagc gacggcaccg
     1021 cccaggcgac tgagttcagg tcgtagccgt cgcagatctg caccagggtg cagagtgcgg
     1081 cgacgcgcaa ttggagcgcg ccgatcttct ggttctcgag cgcggcctcg gccgctgaca
     1141 tcgccgggtt cgccattgtt cggcctttcg acgtgttctc cctgtggccg tgatgttgcc
     1201 ctgcccggtg gcgggcgcgc aagatgaggt ttgacgacga cgcccggagg gaaggcagcg
     1261 catgatcaac aaaatcgtcc agtccatggc ggaggccatg gcgggcatca aggacggctc
     1321 gaccgtgctg atcggtggct tcggcgcggt gggccagccg aatgcgttga tcgacgggct
     1381 gatcgaacag ggggcgaccg acctgacggt cgccgcgaat aacgcgggtg tggggcatgt
     1441 cgggctggcg cggctgatgg agcttgggcg cgtgcgcaag atcatctgtt cgttcccgcg
     1501 gtcgtcggat ccggtggtgt tcgaaagcct gtatcgcgct ggcaagatag agctggagat
     1561 cgtgccgcag ggcacgctgg cggagcggat gcgcgcggcc ggggcgggcg tgccggcgtt
     1621 cttcactgcg accggtgtcg gcaccaagat gggaatgggc aaggaaaccc gcgagttcga
     1681 tggccggagt tatgtcatgg agcgctcgct gccaggcgat gtggcactgg tggaggcgtg
     1741 ggaagccgat cgttggggca acctgacgtt caaggagtcg ggcaggaact tcaatcccgt
     1801 gatggcgatg gcggggaagc tgacaatcgt gcagacgcaa catgtccgcg agctgggtga
     1861 gctcgagccg gaccacgtgg tgaccccggg gattttcgtg gaccgcgtgc tgcacgtgcc
     1921 gtacggcgat cccactggct tctaggaccg cctctgcacc cctgctagtc tcccgccaac
     1981 cacaccttcg gagggaccgc ccatggatgc ccgcactccc caacccggcg agatcaccga
     2041 tcgcaacctc gcccttgaac ttgtgcgtgt caccgaggcc gcggcgatcg ctgcgtccgc
     2101 gtggatcggg ctcggcaaga agaacgaggc cgatggcgcc gccgtggagg cgatgcgcaa
     2161 ggccttcgat caggtcgcga tttccggcac cgtggtgatc ggcgagggcg agatggacga
     2221 agcgccgatg ctgtatatcg gcgagaaagt gggcgccggc ggaccgccga tggacattgc
     2281 cgtcgatccg ctggaaggca cgacgctgac cgcgaagggc ggtccaagcg ctcttgccgt
     2341 agtagcactt gccgagcacg gaaactttct gcatgcgccc gacatctaca tggacaagat
     2401 cgcggtcggc ggcgggttgc cagagggcgt ggtggatctc gatgcggcgg ttggcaagaa
     2461 cctgcgcgaa ctggcccggg caaagcggtg tgaagtgtcg gacctgctcg cctgcattct
     2521 ggaccgcg
//
LOCUS       DATGEM010000263         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1446703, whole genome shotgun sequence.
ACCESSION   DATGEM010000263 DATGEM010000000
VERSION     DATGEM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446703"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="VKQ27_08285"
     CDS             complement(<1..2010)
                     /locus_tag="VKQ27_08285"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HLY88968.1"
                     /translation="MRHIGSAALELLLALVFHGSARAAASGWASNPHGAARLITAVEA
                     AGAGQRVDVALQLRLTPGWHTYWRNPGDAGFAPTIDWSGSDNLARADIAWPAPHRLPS
                     VDGLETIGYVDGVILPIAITLEHPGAPLHLHAEVDYASCKDICIPYHARLDVALPAGL
                     ALPGPDAPLIASARARVPEDPQSANLVLLGATVAPMQGGARLSVQLRSTGAALHAPDL
                     FLEGLPHGSPGRPTVMLDDGGRAASLIVPIRDGAMTALVGATIHATVVDGARAAETDL
                     SPVPGPVPHPNEPRSYLAIAGLALLGGLVLNLMPCILPVLSLKLLALVGYAGAERRAA
                     RLGLLATAAGVVVSFGVLAAILIALKEAGAAIGWGIQFQQPWFLAGMALVTTLFAASL
                     WEWLPIVLPGRLAHAVGGARGRGRFSDAFLLGAFATMLAASCSAPFVGTAVGFALARG
                     PWEIALVFVALGLGMATPFLAIAAVPRLVGWLPRPGPWMRWLQFVLGFALLGTAVWLL
                     FALASETGLQPALLAGAALAVLLGVLAWLAHRASGRRFATLAAVTLAIVTVLAPALRG
                     EAGPVAAPAADASTGLWRPFDEAAVHQMIAQGQVVFVDVTAAWCLTCKANELAVLDRE
                     PVAGRLRGPGVTTVRADWTRPSPAVTALLQHFGRYGVPLDVVYGPD"
     gene            complement(2022..2198)
                     /locus_tag="VKQ27_08290"
     CDS             complement(2022..2198)
                     /locus_tag="VKQ27_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88969.1"
                     /translation="MPEHGMQMNGIRGQGGHHSMMSGGMMERCADMMQSMNGEDSRPN
                     SQWQNPPSGLAAPP"
     gene            complement(2627..>2953)
                     /locus_tag="VKQ27_08295"
     CDS             complement(2627..>2953)
                     /locus_tag="VKQ27_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HLY88970.1"
                     /translation="EGYATYLEQNGFKVDIRPTNDLAEISRKAGVPVELEGCHTSFIG
                     DYVVDGHVPVPSIHKLLDGKTDVAGITLPGMPPGSPGMFGEKTGPLTIYAISRDGTPP
                     RIFDVI"
BASE COUNT          600 a          962 c          944 g          447 t
ORIGIN      
        1 gtctgggcca tagactacgt cgagcggtac tccatagcgg ccaaagtgtt gcagcagcgc
       61 cgttaccgcg ggggatgggc gtgtccagtc cgcgcgaacc gtcgtcacgc ctggaccacg
      121 aagtctgcct gcgaccggct cccgatccag cacggccagc tcgttcgcct tacaggtgag
      181 gcaccaggcc gcggtgacat caacgaacac gacctgtcct tgcgcaatca tctggtgaac
      241 agccgcctcg tcaaaggggc gccataatcc agtcgaggca tcggctgccg gggccgcaac
      301 cggtcccgcc tctccccgca gggcaggcgc gagcacggtg acaatggcca gcgtcacggc
      361 ggctagcgtg gcgaagcggc ggccagaagc gcggtgggcc agccaggcga ggactcccag
      421 caggacagcc agagcggcgc cggcgaggag tgcgggctgc agacctgtct cggacgcaag
      481 cgcgaacagc agccatacag cggtgccaag caacgcgaac ccgagcacaa actgcagcca
      541 gcgcatccac gggcccggcc gtggcagcca accaacgagc ctgggaaccg ctgcaatggc
      601 aagaaacggc gttgccattc caagcccgag agcgacgaat accaaagcga tctcccaagg
      661 accgcgtgcc aaggcgaagc caaccgcagt gccgacgaag ggcgccgaac aggaggcagc
      721 gagcatggtt gcgaacgcgc cgagcaggaa agcatcactg aaccggccac gaccgcgggc
      781 accaccgacg gcatgcgcca accggccagg caggacgatc ggcaaccatt cccaaagact
      841 ggcggcgaat agcgtcgtca cgagtgccat acccgctaga aaccacggtt gctgaaactg
      901 gatcccccag ccgatggccg caccagcctc cttcaacgca atcaggatcg cagccagcac
      961 gccgaaggac acgaccacac cggcagccgt ggcgagaagg ccgaggcgtg cggcgcgccg
     1021 ctccgcgccg gcataaccga ccagagccag cagtttcagc gacagcaccg gtaggatgca
     1081 gggcatgaga ttgagcacga gccccccgag cagcgcgaga ccagcaatgg caaggtaact
     1141 ccttggctca ttagggtgcg ggactggacc cggcaccggt gacaggtccg tctccgcggc
     1201 gcgtgcgccg tcgaccaccg tcgcgtggat cgtggcgccc accagtgccg tcattgcacc
     1261 atcgcggatt ggcacaatca ggcttgccgc tcggccgccg tcgtccagca tcaccgtggg
     1321 tcgaccaggc gagccatgag gaagaccttc caggaacagg tcgggcgcat gcaaagctgc
     1381 tccggtgctc ctcaattgaa cggatagccg ggcgcccccc tgcattggtg cgaccgtcgc
     1441 cccgagcaaa acaaggtttg cagactgcgg atcctccggc acgcgtgccc gtgcagacgc
     1501 gattaacggt gcgtccggac caggaagagc cagcccggca ggcagggcca catcgaggcg
     1561 cgcgtggtag gggatacaga tatccttgca cgatgcatag tccacctcgg cgtgcaagtg
     1621 gagcggcgct cccgggtgct ccagtgtgat cgcgatcggc agaatgacgc cgtccacata
     1681 gccgatcgtc tcaagaccat cgacggatgg caggcgatgc ggtgcgggcc aggcgatgtc
     1741 ggctcgcgcc agattgtccg atcccgacca atcgatcgtg ggtgcgaagc ccgcatcgcc
     1801 cgggttgcgc caataggtat gccatccggg cgtgaggcgc agttgcagtg cgacatcgac
     1861 gcgctggccg gccccggcgg cctcaaccgc cgtgatcagc cgggccgcgc catgcggatt
     1921 gcttgcccag ccgctggctg ccgcgcgcgc cgaaccgtgg aatacaagcg ccagcagcag
     1981 ctccagcgca gctgaaccaa tgtgacgcat aggcgtgcgg ctcaaggagg tgccgcaagg
     2041 ccggacggcg gattttgcca ttggctgttt ggccggctgt cctcgccgtt catcgactgc
     2101 atcatgtcgg cgcagcgttc catcatgcca cccgacatca tgctatgatg tccgccttgt
     2161 cctcttatgc cgttcatttg catgccatgc tcgggcattg agcctgcatg gggatcggca
     2221 aatgcaggga gggtcgacca tccgacagca gcgaacagcg caacgaaggc gacagcacgc
     2281 gatagcctca tgtcctgtgt ccttcctgaa tttcaagcgg aggagtcgaa gtcgtcgtct
     2341 cggtaagttc gcgcgacgca tccgtgtttg atgcatggca tgaaccgcca cttgcgcgat
     2401 gcatacagag ccccagcgcg cacatcgccg cacaaggggc gaggctcagc aggattggcg
     2461 caacgccgat cgccacgagc cagttccact gcagtgccat tgcggctgca atgacgacca
     2521 atgcaacaat gccgaagaca agccgatgcc gtccgagcca cgcgatgaag ttgcgcgccg
     2581 ctggtggggt cggagagaca aaagtcgctt ccatggtttg atctgcctag atcacgtcga
     2641 agatgcgcgg cggcgtaccg tcgcggctga tcgcatagat ggtgagcggc ccggtcttct
     2701 cgccgaacat gccgggcgag cccggcggca tgcccggcag cgtgatgccg gcaacatcgg
     2761 tctttccgtc gagaagtttg tggatgctcg gtaccggcac atggccgtcc acgacgtagt
     2821 caccgatgaa acttgtatgg cacccctcaa gctctacggg aacgccggcc ttgcggctga
     2881 tctcggcaag gtcgttcgtt ggtctgatat ccaccttgaa gccattctgc tctagatacg
     2941 tcgcgtagcc ctc
//
LOCUS       DATGEM010000264         7945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_704096, whole genome shotgun sequence.
ACCESSION   DATGEM010000264 DATGEM010000000
VERSION     DATGEM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704096"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VKQ27_08300"
     CDS             complement(<1..437)
                     /locus_tag="VKQ27_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88971.1"
                     /translation="MTKAPMTVISGKYAVGITLTSKAQNPVTVTGTITPSNSIALYGP
                     GGGTNSWTISNAGSIGGGAHTGIALGDTIMYVATGIVTNQAGGRISGYDIGVNIGGPG
                     TLTNAAGGTIIGSHSDGVYFGSLAPATVINNGVLIGGFGGEYMR"
     gene            561..1346
                     /locus_tag="VKQ27_08305"
     CDS             561..1346
                     /locus_tag="VKQ27_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88972.1"
                     /translation="MPGKLRSILALLLPLLIAQGAVAQPTEDARLACDRAAAQAEHTW
                     NLPPGLLAAIGTVESGRIDPVALDRHAWPWSINADGAGYFAASKSEAIGLVRVLQARG
                     ARYIDVGCFQVDLFYHPDAFNSLEQAFDPDANAGAAARILVQGRFNATSWDQAVAAYH
                     SASLLKGAWYLQRVLAAWHGTRNFLLPAPGVSPGDRPGYVVLLSPAAKLVRVVTPSGS
                     SPVPVAGLPRVISPSAVPAQLEPAGLPRVLTHSEAMPRVGGRL"
     gene            1343..2185
                     /locus_tag="VKQ27_08310"
     CDS             1343..2185
                     /locus_tag="VKQ27_08310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY88973.1"
                     /translation="MSRAPISGFVIAYNRASLVGTSLRSLRFVDELIVIDKSSTDDTP
                     AIARRYADKVITVPWSPTVEETRAFALEQCRHDWIAFLDDDEMLNPAAIAHLHAGPTA
                     DAVALPLRHWILGAFDPEAYYWPEHHIRFFRKGAVSFRPVVHGGMQLHAGSVERVAPK
                     TQIFIEHLSHRDAEQWIERTNRYTSRPSRVNVEAEGTDLIDFAHRRIDHWLARGGHTD
                     GDDYPAAVALLRAIYDMVDRIKAWETQRGIDGAAAFRARCAELEQAYDALETSLGIAT
                     GMHR"
     gene            2182..3039
                     /gene="uppS"
                     /locus_tag="VKQ27_08315"
     CDS             2182..3039
                     /gene="uppS"
                     /locus_tag="VKQ27_08315"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HLY88974.1"
                     /translation="MSELIARQPAVSPIALSLHRWRRAVVGSITHPLYALYSRRLLAQ
                     VMLRQPPRHVGIILDGNRRFGRLSGMIDPMQVYAKGAQKLDDLLDWCGELSIPAVTLW
                     VCSTDNLAREPKQVAGILGALEAKLRALVDDPQIHRRRVRVQAIGRLDLLPESTIEAI
                     RAAESATAAYDAMLLSIAVAYGGREEIADAVREMLSEAQQNGASLADVIDDVCPERIG
                     RHLYLSEAPEPDLIIRTSGELRLSGFLLWQSCYSEFYFSDVLWPAFRKIDFLRAVRAF
                     QQRKRRYGR"
     gene            complement(3027..4052)
                     /locus_tag="VKQ27_08320"
     CDS             complement(3027..4052)
                     /locus_tag="VKQ27_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY88975.1"
                     /translation="MGEIFRRETAEHPEPFTGERLTTAIGGQVQIEHYHRYLFARSLV
                     AGLDVLDVASGEGYGSALLAQVARTVVGVEYSGRTACGAAMNFRRPNLYFMRGDARAL
                     PLVDASVDVVVSFETIEHFDHQTDFVCEVHRVLRPGGCCIVSTPDRDVYSPAGAAANP
                     FHVHELSGAEFVALLHTRFRHVSIVRQRAMIGSALLPDTIPEAPPLVFDRRGDTHFEA
                     CTGLPRAPYLVAVASDDEPPALPPSVYISRSDVDTDLLSAMALAKQVHETKAKLDETQ
                     AKLDDAQAKLDDTQTRLRDVTQRAEGAERERVEAEQRFALLRGSLRTFLRGYLPLLRA
                     HLFGQRP"
     gene            complement(4057..4884)
                     /locus_tag="VKQ27_08325"
     CDS             complement(4057..4884)
                     /locus_tag="VKQ27_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HLY88976.1"
                     /translation="MTDPRDALEAIGVIPDTEIDIGNAAIQLARVDAPNADWQAARAQ
                     LSELARDAVAIAKSLDTRDVSMRAEALAGLISGRYAFAGDSETYDDLANANMIRVVER
                     RKGLPVALGIIWLHAARAAGWGAHGVDFPAHFLIALEGKSVQAVVDVFAGGMTLDARD
                     LRVLLKRVEGEDAELRPGLLRPMSARRVLLRLQNNIMTRRLQAGDLAGGLTCTEDMLR
                     IAPDHAELWRQAAVMNQRLDRVGAALRCFDRFLALVPEGDAAARVRGAMNELRTRLN"
     gene            complement(5055..6881)
                     /gene="thiC"
                     /locus_tag="VKQ27_08330"
     CDS             complement(5055..6881)
                     /gene="thiC"
                     /locus_tag="VKQ27_08330"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HLY88977.1"
                     /translation="MTIQSKTAARPSSVTTGAIAGSRKIYTSPGGRPDINVPFREIAL
                     EPTAREEPYRAYDTSGPYTDPAVTIDLEAGLLPVRAGWIAKRGFERIAPRAVKDVDNG
                     GASGDKLVPTCPAEHTVLAGRPGKPVTQFEFARAGIITEEMIYVAHRENLGRQKALEG
                     AAERVADGESFGAEIPEFITPEFVRDEIARGRAIIPANINHPELEPVIIGRNFLVKIN
                     ANIGNSAVTSGAAEEVEKLVWATRWGADTVMDLSTGRNIHNIRGWILRNSSVPIGTVP
                     IYQALEKVDGEPDKLDWEVFKDTLIEQAEQGVDYFTIHAGVRLGYVPLTAKRVTGIVS
                     RGGSIMARWCLAHHKESFLYERFDEICDIMRKYDVSFSLGDGLRPGSNADANDAAQFA
                     ELETLGELTKIAWDKGCQVMIEGPGHVPMHKIKVNMDKQLRECGEAPFYTLGPLTTDV
                     APGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNRDDVKVGVITYRIAAHAADLA
                     KGHPSAKLRDDAISRARFEFRWQDQFNLSLDPDTARNFHDETLPKEAHKVAHFCSMCG
                     PKFCSMKITQDLRVEAERMAGLEQKSKEFAEHGKEIYLPAAE"
     regulatory      complement(6935..7066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7172..7288
                     /locus_tag="VKQ27_08335"
     CDS             7172..7288
                     /locus_tag="VKQ27_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLY88978.1"
                     /translation="MDTAVNIRLDADVLAFFRASGPGWQSRINAALRKVVGI"
     gene            complement(7437..7559)
                     /locus_tag="VKQ27_08340"
     CDS             complement(7437..7559)
                     /locus_tag="VKQ27_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HLY88979.1"
                     /translation="MRLPKSLLDTVKTSTQKAAVPYQQFIRLALEAAVGSCGAR"
     gene            complement(7645..7824)
                     /locus_tag="VKQ27_08345"
     CDS             complement(7645..7824)
                     /locus_tag="VKQ27_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HLY88980.1"
                     /translation="MKRRHLVKRYTLARLYDTTTACYVDVAQLRALVQAGDEVTVLDA
                     KTQKDITSEFLRISP"
BASE COUNT         1380 a         2691 c         2424 g         1450 t
ORIGIN      
        1 cgcatgtact cgccgccaaa cccgccgatg agaacgccgt tgttgatcac cgtcgccggg
       61 gcgaggctgc cgaaatacac accgtccgag tgcgagccga tgatggtgcc gcccgcggca
      121 ttggtcagcg tgcccggccc gccgatattg accccgatat cgtacccgga gatccggccg
      181 cccgcctggt tggtgacgat ccctgttgcg acatacatga tggtgtcgcc cagcgcgatg
      241 ccggtgtgag caccgccacc gatcgatccc gcattggaga tggtccaact gttcgttccg
      301 ccaccgggcc cgtacagcgc aatgctattc gatggcgtga tggtgccggt gaccgtgacc
      361 gggttctgcg ccttgctggt cagtgtgatg ccaacagcat atttgccgga aatgacggtc
      421 atcggagcct tggtcaatgc agcgatcgcc aggagtgacc gataacggtc gccgcattaa
      481 cgatcggtga aggcccgtcg catagtttct gtcttgtgat cgatcactca ccataaccac
      541 catcccggcc tcaccgtccc atgcccggca aactgcgctc catcctggct ttgttgctgc
      601 cactgttgat cgcacaggga gctgtcgcgc agccgacgga ggacgcccgt ttggcctgcg
      661 atcgcgccgc tgcccaggca gagcacacct ggaacctgcc acccggactg ctcgcggcaa
      721 tcggtacggt agagagcggc cggattgatc cggttgcatt ggatcgacat gcctggccct
      781 ggagcatcaa tgccgacggc gccggctatt tcgccgccag caaatccgag gcaatcggcc
      841 tggtgcgtgt gttgcaagcg cgcggcgccc gctacatcga tgtcggctgc ttccaggtgg
      901 acctgttcta ccaccccgac gcgttcaact ccctggaaca ggcgttcgat cccgacgcca
      961 acgccggcgc cgctgcgcgt atcctggtgc agggccggtt caacgccaca agctgggacc
     1021 aggccgtcgc ggcgtaccac tcggcctcac tgctgaaggg cgcctggtac ttgcaacggg
     1081 tgctggcagc ctggcatggc acgcgtaact tcctcctccc tgcgcccggc gttagcccag
     1141 gggatcgccc cggttacgtc gtcttgctgt ctcccgcggc aaagctggta cgggtcgtca
     1201 ctccctcggg ctcgtcgccg gtaccggtcg ctgggctgcc ccgcgtcatt tcaccgtccg
     1261 ccgtgcccgc gcaactcgaa ccggcggggc tgccgcgcgt cctgacgcac tccgaagcca
     1321 tgccacgcgt tggtggccgc ctgtgagccg cgcgccgatc agcgggttcg tgatcgccta
     1381 caaccgggca tcactcgtcg gcaccagcct gcgctcgctg cgcttcgtcg acgaattgat
     1441 cgtgatcgat aagtcatcga ccgacgacac gccggcaatt gccaggcgat acgccgacaa
     1501 ggtgattacg gtgccatggt cgcctacggt tgaagaaacg cgcgccttcg ccctggaaca
     1561 atgtcgccac gactggatcg ccttcctgga tgacgacgag atgcttaacc ctgcggcgat
     1621 cgcccacctg cacgccggcc ccaccgccga cgccgtcgcc ctgcctctgc ggcattggat
     1681 cctgggagcg ttcgatccag aggcctatta ttggcctgag caccacatcc ggttcttccg
     1741 caagggcgct gtaagctttc gaccggtagt gcacggtggc atgcagctcc acgccggcag
     1801 tgttgaacgt gtcgccccga agacacagat cttcatcgag catctgtcgc atcgcgacgc
     1861 ggagcagtgg atcgaacgga ccaaccgtta tacatcacgt ccaagtcggg tgaatgtaga
     1921 ggctgagggg accgatctga tcgacttcgc tcatcgccgc atcgaccact ggctcgcccg
     1981 cggcggccat accgacggcg acgactatcc tgccgcggtc gcattgctgc gcgcgatcta
     2041 cgacatggtc gaccgcatca aggcatggga gacgcagcgc gggattgatg gcgccgccgc
     2101 gttccgcgcg cgctgcgccg agctcgaaca ggcctatgac gcattggaga caagcctggg
     2161 catcgcgacc gggatgcacc gatgagcgaa ctgattgccc ggcaacccgc ggtcagtccc
     2221 atcgctctgt cactgcatcg ctggcgccgc gcggtggtgg gctcgatcac ccaccccctc
     2281 tacgcgctct acagccggcg gctgctcgcc caggtgatgc tgcgccaacc gccgcgtcac
     2341 gttggcatca tcctggatgg gaaccgccgc ttcggccgcc tctccggcat gatcgatccc
     2401 atgcaggtct acgccaaggg cgcgcagaag ctggatgacc tcctggactg gtgcggcgag
     2461 ttatccatac cggctgtaac gctctgggtc tgctccaccg acaatctcgc gcgcgagccg
     2521 aagcaggttg cgggcatcct cggtgcgctg gaggccaagc tgcgcgcgct ggtcgacgac
     2581 ccgcagatcc atcgccgccg cgtccgcgtc caggcgatcg ggcggctcga cctgctgccg
     2641 gagagcacga tcgaagcgat ccgcgccgcg gagagcgcta ccgcagcgta tgatgccatg
     2701 ctgctctcca tcgcggtcgc ctatggcggg cgcgaggaaa tcgccgacgc cgtgcgcgag
     2761 atgctgagcg aggcgcagca gaatggcgcc agcctggccg acgtcatcga tgacgtgtgc
     2821 cccgaacgga tcggccgcca tctgtatctc tccgaagcgc cggagcctga cctgatcatc
     2881 cgcaccagtg gcgagcttcg gctgtccggc ttcctgctct ggcaaagctg ctacagcgag
     2941 ttctacttct ccgacgttct ctggcccgcc ttccgcaaga tcgatttcct gcgcgccgtg
     3001 cgggcattcc aacagcgcaa gcggcgctac ggccgctgac cgaacaggtg cgcgcgcagc
     3061 agcggcaggt aaccgcgcaa gaatgtgcgc aacgagcctc gcagtaatgc gaatctctgt
     3121 tccgcctcga cgcgctcgcg ctccgctccc tccgcgcgct gcgtcacgtc ccgcagcctc
     3181 gtctgcgtgt catcgagctt cgcctgcgcg tcatccaact tcgcctgagt ctcatccagc
     3241 ttcgcctttg tctcatgcac ctgcttcgcg agagccatgg cgctgagcag gtcggtatcc
     3301 acatcactcc ggctgatata cacgctcggc ggcagcgccg gtggttcatc gtctgacgca
     3361 accgcgacca gatagggtgc gcgtggcagc ccggtgcaag cctcgaaatg cgtgtcgccg
     3421 cgccgatcga acaccagcgg tggcgcctcc ggtatcgtat cgggcaggag cgccgaaccg
     3481 atcatcgcgc gctgccgcac gatcgagaca tgccggaatc tggtgtgcag caaagcgaca
     3541 aattccgcgc cgctcagttc atgcacatgg aatggattgg ccgccgctcc agccggcgag
     3601 taaacgtcgc gatccggcgt gctgacgatg caacagccac cgggccggag cacgcggtgc
     3661 acctcgcaaa cgaaatccgt ctgatggtcg aaatgctcta tggtttcgaa tgatacgacc
     3721 acgtcgacgc tggcatcgac gagcggcagg gctcgcgcgt caccgcgcat gaaatacaaa
     3781 ttcggccgac ggaaattcat cgcggcaccg catgccgtcc gtcccgagta ctccacgccg
     3841 acaacagtcc gtgcgacctg cgccagcaac gccgaaccgt agccctcgcc actggcaaca
     3901 tcgagcacgt cgagaccggc gaccagcgat cgcgcgaaaa ggtagcggtg gtagtgttcg
     3961 atctgcacct ggccgccgat agcggtggtc aggcgctcgc ccgtgaaggg ctcgggatgc
     4021 tccgccgttt cgcggcgaaa gatctcaccc attgccctag ttgagccgcg tccgcagctc
     4081 gttcatcgcg ccgcgcacgc gtgccgctgc atcgccttcc ggcaccagcg caaggaaccg
     4141 atcgaagcag cgcagcgcgg cgccgacgcg atccagccgc tggttcatca ccgccgcctg
     4201 acgccacagc tccgcatggt ccggcgcgat gcgcagcatg tcctccgtgc aggtcagccc
     4261 gccagccagg tcgccggcct gcagccgccg cgtcatgatg ttgttctgca atcgcagcag
     4321 cacgcgccgc gcgctcatcg gccgcaaaag cccagggcgc agctcggcat cctcgccctc
     4381 cacgcgcttc agcaaaaccc gcagatcacg cgcatcgagc gtcattccgc cggcgaacac
     4441 atcgaccacc gcctgcacgc tcttcccttc cagcgcgatc aggaaatgcg caggaaagtc
     4501 caccccgtgc gcgccccatc cggcagcgcg ggccgcatgc agccagatga taccaagcgc
     4561 caccggcaag cctttgcggc gctcgaccac ccggatcatg ttggcattcg ccagatcgtc
     4621 gtaggtttcg ctgtcaccag cgaacgcgta gcggccagag atcagcccgg ccaacgcctc
     4681 cgcacgcatc gacacatcgc gggtgtcgag cgactttgcg atcgccaccg catcgcgtgc
     4741 gagctcggaa agttgtgccc gtgcggcctg ccaatcggcg ttcggggcgt caacgcgcgc
     4801 aagctggatc gcggcattac cgatatcaat ctcggtatcc ggtatgactc cgatcgcttc
     4861 caaagcatcg cgcgggtccg tcatccccgc agtgtggggt tacacgttac gccgcgcaag
     4921 agttggcagt acggccccag cgtcgtctca atgtcattgc gaggaggcaa agccgacgaa
     4981 gcaatctcgc gcagattgcg cgcgcacggg ccctcaggat tgcttcgtgg ctacaccccc
     5041 tcgcaacgcc aggatcattc cgccgccggc agatagatct ccttgccgtg ctcagcgaac
     5101 tccttgctct tctgctccag ccccgccatg cgctccgcct ccacccgcaa gtcctgcgtg
     5161 atcttcatcg agcagaactt cggcccgcac atcgagcaga aatgcgcgac cttgtgtgct
     5221 tccttcggca gcgtctcgtc atggaagttg cgtgccgtat cagggtcgag cgaaaggttg
     5281 aactgatcct gccaacggaa ctcgaaccgc gcccggctga tcgcatcgtc gcgcagcttg
     5341 gcactcggat gccccttcgc cagatccgcc gcatgggccg cgatccggta ggtgatcacg
     5401 cccaccttca catcgtcgcg gtttgggaga ccgagatgct ccttcggcgt gacatagcaa
     5461 agcattgcgg taccgaacca gccgatcatc gcggcaccga tcgctgaggt gatgtggtcg
     5521 tagccaggtg ccacgtcggt cgtcagcggg ccaagcgtgt agaacggtgc ttcgccgcac
     5581 tcgcgaagct gcttgtccat gttgaccttg atcttgtgca tcggcacgtg gccggggcct
     5641 tcgatcatca cctggcagcc tttgtcccag gcgatcttgg tcagctcgcc cagcgtctcc
     5701 aactcggcaa attgcgccgc gtcgttcgca tcggcgttgg agccaggccg cagcccgtcg
     5761 cccagcgaga acgacacatc gtatttgcgc atgatgtcgc agatctcgtc gaagcgctca
     5821 tacaggaagc tctccttgtg atgcgccagg caccaccgcg ccatgatcga gccgccgcgt
     5881 gacacgatgc cagtgacacg cttcgcggtg agcggcacat aaccgagacg cacgccggca
     5941 tggatggtga agtagtccac gccctgctcg gcctgctcga tcagcgtgtc cttgaacacc
     6001 tcccagtcga gcttatcagg ctcgccatcc accttctcca gcgcctggta gatcggcacc
     6061 gtgccgattg gcaccgacga attccggaga atccagccgc ggatgttgtg gatgttgcgc
     6121 ccggtggaaa ggtccatcac cgtgtcggcg ccccaacgtg tcgcccatac cagtttttcc
     6181 acctcttcgg ccgcgccgga cgtcaccgcc gagttgccga tattggcgtt gatcttcacc
     6241 aggaagttgc ggccgatgat gaccggctcg agctccgggt ggttgatgtt cgccgggatg
     6301 atggcgcggc cgcgcgcgat ctcgtcacgc acgaattccg gcgtgatgaa ttccggaatt
     6361 tcggcgccaa aactctcgcc atccgcgacc cgttcggcgg caccttccag cgccttctgc
     6421 cgcccgagat tctcgcggtg cgcaacatag atcatttctt cggtgatgat gccggcacgg
     6481 gcgaactcga actgcgtcac cggcttgccc ggccggccgg cgagcaccgt gtgctccgcc
     6541 ggacatgtcg gcaccagctt gtcgccgctg gcgccaccat tgtccacgtc cttcaccgcg
     6601 cgtggcgcga tgcgttcgaa gccgcgcttg gcgatccatc cagcgcgtac cggcagcagg
     6661 cccgcttcca gatcgatcgt gaccgccgga tcggtatacg gccccgacgt atcataggcg
     6721 cgatacggtt cctcgcgcgc ggttggctcc agcgcgatct cgcggaacgg cacgttgatg
     6781 tcagggcgtc ccccgggcga agtgtagatc ttgcgcgagc cggcgatcgc gccggtggtg
     6841 acgctgctcg gacgggcggc ggtcttcgac tggatagtca tggacgtctc ctcaaacgca
     6901 cacgtgggtt tccgcgggcg gaggaagggt gttgcgatgg ctgtagcacc cgtccctccg
     6961 ccggcatgac ccggttcagg ttcaagggtc accgcgccgc cgctcgtggg agcgggagaa
     7021 ggcggtatct cagcccctga acggcggggc ccccctcggt agtcggcaac aatgagggcg
     7081 ggtggaggcg gtgtcaaatg gatgacgggc gatgttcacc ggttggtagg aggccgcggc
     7141 ctaggaggtt gccctgacgc ggccggtttt tgtggataca gccgtcaaca ttcgcctcga
     7201 tgccgatgta ttggcgtttt tccgggcgag cgggcctggg tggcagtcgc ggatcaatgc
     7261 ggcgttgcga aaggtcgtgg gcatataggt gccgtcgaca agatgtctac tcaaatggcc
     7321 acatgacggt tgaggaggtc gctttcttca tgcgattatt gtcctcgcgc ctcgcgcgca
     7381 tctttgatga cttttacgcg acgagccaac gagcttagcc ggggcaggcc tcgaactcag
     7441 cgtgcgccgc acgaaccgac agcagcctcc aacgccagcc ggataaactg ctgatagggg
     7501 acggcggcct tctgcgtgga cgtcttcacc gtatccagca acgacttcgg cagccgcata
     7561 tcccatggat atccccggta caatttctgg gacgcatcac tgggaaaagg cacgctccca
     7621 aaactggccg caccgggggc acggttacgg cgaaatccgc agaaactccg aggtaatgtc
     7681 cttctgtgtc ttggcgtcca acaccgtcac ctcgtcaccg gcctgcacca gcgcccgaag
     7741 ctgtgcgaca tcgacatagc aagccgtcgt ggtgtcatag agccgggcga gggtataccg
     7801 cttgacgaga tgccggcgct tcattggtgc gttctgtgcc atccggcgag tatggcacgg
     7861 attcatcggg ccgcgcgacg tcatagcccc tgttcgcaac aagcggattg acgccgccgc
     7921 gcacgcccct catttcctcc cgcca
//
LOCUS       DATGEM010000265         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_370572, whole genome shotgun sequence.
ACCESSION   DATGEM010000265 DATGEM010000000
VERSION     DATGEM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370572"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VKQ27_08350"
     CDS             <1..937
                     /locus_tag="VKQ27_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HLY88981.1"
                     /translation="AHPQYYERAGDDGRVIKRPGTPRQGKVGIVSGGGSGHLPIFTGY
                     VGKGLLDACAIGEVFSSPSVDQMAGAMRVANAGAGVLRLYGNYGGDCMNFDMAGDIME
                     MEDVRSTTVLLTDDVASAPPAEKQKRRGVAGMVYAFKMAGAAAEAGANLDEVTRIAQN
                     AADSVRSIGAALTPCIVPQAGRPTFTIADDEMEMGMGIHGEPGVWRGKLRPADAVANE
                     MMDFLLKDMRIARGDRVSILVNSLGATPPEELYILYRIVAARLGDVGATIVMPLVGRY
                     ATSMEMAGVSFTLCKLDDELEKLLHAPCECAFWRV"
     gene            939..1541
                     /locus_tag="VKQ27_08355"
     CDS             939..1541
                     /locus_tag="VKQ27_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HLY88982.1"
                     /translation="MALTTPILADGLKRIATHMETVADDLNALDGQLGDGDLGVTMTR
                     GGRVLVDILPKLPADVGMALMACAQAMTKVSGSSYGTLLATGLLAVAKATRGRTEVPW
                     SETAALLRTALEAMMTRGKASLGDKTVLDALDAAATAAEGKTDPGQMLQAATAAVEAT
                     LDRMRNMQAKIGRARIFGERSVGLDDPGMRAFRDILRGLS"
     gene            complement(1615..2208)
                     /locus_tag="VKQ27_08360"
     CDS             complement(1615..2208)
                     /locus_tag="VKQ27_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLY88983.1"
                     /translation="MPASLIQALRAMAYNNAWANHRLLSACAGLSQAEFEAPRTGFFP
                     SIQATLNHILVVDWFYVDALEGGTLGPAAWANPVPCRTVPELQVAQAAVDRRLIAWCD
                     GLDEDGVELIIQVHRDTRVQTERADRLLLHMFQHQVHHRGQAHAMLSATTSVAPPQLD
                     EFFSIGEAPLRAAEFSELGWTEATVWGEGGTAPPKGT"
BASE COUNT          413 a          786 c          713 g          363 t
ORIGIN      
        1 ggctcatccg caatactacg agcgcgccgg tgatgatggc cgcgtgatca agcggcccgg
       61 cacgccgcgc caaggcaagg tcggcatcgt ctccggtggc ggctccggac atctgccgat
      121 cttcaccggc tacgtcggca aggggctgct ggacgcctgt gcgatcggcg aggtgttctc
      181 ctcgccgtcg gtcgatcaga tggccggggc gatgcgcgtg gcgaacgccg gcgccggtgt
      241 gctgcgcctc tatggcaact acggcggcga ctgcatgaac ttcgacatgg ccggcgacat
      301 catggaaatg gaggacgtgc gctccaccac cgttctgctg accgacgacg tcgccagcgc
      361 gccgccagcg gaaaagcaga agcgccgtgg cgtcgccggc atggtttacg cattcaaaat
      421 ggcgggtgcc gcagcagaag ccggcgccaa cctcgacgaa gtgacccgca tcgcgcagaa
      481 tgccgctgac tctgtgcgca gcatcggtgc cgcgctgacg ccgtgcatcg tgccacaggc
      541 cggccggccc accttcacca tcgctgacga cgagatggaa atgggcatgg gaatccacgg
      601 cgagcccggc gtctggcgag gcaagctacg tcccgcggat gcggtggcga atgagatgat
      661 ggacttcctg ctcaaggaca tgcggatcgc gcgcggtgat cgcgtgtcca tactggtgaa
      721 cagcctcggt gccacgccgc ccgaggaact ctacatcctg tatcgcatcg tcgccgcacg
      781 gcttggcgac gtaggcgcga caatcgtgat gccgctggtc ggccgctacg ccacatcaat
      841 ggaaatggca ggcgtatcgt tcacgttgtg caagctcgac gatgagcttg aaaaactgct
      901 gcacgcgccg tgcgagtgcg cgttctggcg ggtgtgacat ggcactcacc acaccgatcc
      961 tggctgacgg cctcaaacgc atcgccacgc acatggagac ggtggcggac gaccttaacg
     1021 cactcgatgg ccagctcggc gacggcgacc tcggcgtcac gatgacacgc ggcggacgcg
     1081 tgctggtgga catcctgccg aagctgccgg cggatgtcgg catggcactg atggcctgcg
     1141 cgcaggccat gaccaaggtg tccggctcca gctacggcac gctgctcgct accggcctgc
     1201 tcgccgtcgc gaaggccacg cgcggccgca ccgaggtgcc atggtccgaa accgccgccc
     1261 tgttgcgtac tgcgctggaa gccatgatga cgcgcggcaa ggcctcgtta ggcgacaaga
     1321 cggtgctgga tgcgctggac gccgcggcta cggcagccga aggcaagacc gatcctgggc
     1381 aaatgctgca agccgcgact gccgccgtgg aagcgacact ggaccgcatg cgcaatatgc
     1441 aggcgaagat cggccgcgcc cgcattttcg gcgaacgctc cgttggactg gacgatcccg
     1501 gcatgcgagc cttccgcgac atcctgcgcg gcttatcttg acattcgtgt atacgccaac
     1561 tcttcccaac cgtcatcgcc gggcatgacc cgggccatca gaagcgatcg gtacctacgt
     1621 gcccttggga ggcgctgtac ctccctctcc ccacaccgtc gcttcagtcc accccaactc
     1681 ggaaaattcc gccgcccgca acggcgcctc gccgatcgag aagaactcat cgagctgcgg
     1741 cggcgccact gacgtcgtcg cactcagcat cgcatgcgcc tgcccgcgat gatgcacctg
     1801 atgttggaac atatgcagca acagccgatc cgcccgctcc gtctgcacac gtgtgtcacg
     1861 atgcacctgg atgatcagct ccaccccatc ctcatccagc ccgtcgcacc acgcgatcag
     1921 ccgccgatcc accgccgcct gcgccacctg cagttccggc accgtccggc atggcacagg
     1981 gttcgcccat gcggccggtc ccagcgtgcc cccctccagc gcatcgacat agaaccagtc
     2041 gaccaccagg atgtggttca gcgtcgcctg tattgacgga aagaaaccgg tgcgcggcgc
     2101 ctcgaactca gcctggctca ggccggcaca cgccgacagc aggcgatggt tggcccaggc
     2161 gttgttgtac gccatggcgc gcagcgcttg aatcagcgag gccggcacga gccagcctca
     2221 gtgtccgacc tccactaccg cgtcggcagc gaacgccccc tgcccgtccg gcatc
//
LOCUS       DATGEM010000266         4559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1101044, whole genome shotgun sequence.
ACCESSION   DATGEM010000266 DATGEM010000000
VERSION     DATGEM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101044"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..3141
                     /locus_tag="VKQ27_08365"
     CDS             340..3141
                     /locus_tag="VKQ27_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY88984.1"
                     /translation="MTTTAEENKPYIFGSFELVPERRQLLNSGKPVNLGSRAFDVLIT
                     LVKSRGDTVSNATIMARAWPATTVDEASVRVHISALRKLLGDGRGTNRFIANVPGRGY
                     VFVAPVTRGQELTAAKPSDQLPPRSLPTPAADIVGRAPVIARLVALLSRHRFLTIVGI
                     GGIGKTTVALAVSEAAADAYPDGTWFVPLASLPTSSLVSSAVGGALGLATTGPDTLPV
                     LTASLRDKQALIVLDNSEHVTDGAAEVAEAILKAAPRVSILVTSRIPLRAEGEWRHRL
                     EPLETPSHHDDITAAEALAHPAVELFYERARASSGEFAITDANAPLVCEVCRRLDGVP
                     LALELAAAQIGTLGISVLARGLNDRFALLTLGHRTALPHQQTLRATMDWSYDLLTATE
                     QRVLQRLAVFRGDFTTEAAFAVAGDAELAGSAVLLHIASLATKSLLATDISGDVTYFR
                     LFETTRIYAIERLKETAALDDVARRHAEYFLALLATLDEERQAQPPDAHTALLRRHGD
                     EIHAALDWAFSPTGDTGIGLALTLAAIPLWFELFQLVVAHARLEQALDHAAPDSEDEM
                     RLRIAIGHALWYIGSGTAAIEPTFTRVLEIAEPIGATTAQTQALWGLWASCRGRGDYP
                     AALTMARRFADAADRTGDVSAMHLADRILGLTNHFLGDQPIARSFTERALRHPEHLDS
                     SLGLGYQVETPVAMAAQLARILWLQGFPDRAKLAATDAIEAARQSGHPFAMVYGLAFG
                     SASVALWTGDIPEATRVIERLIARTAGNRRTEQWVRYFASVLKLRGGNDTDALVASFV
                     EPRVDLFPVQLFDELVTQGTIPVPMPGPEPSQVLWNTAELLRIDAELLLWHNGPDAAA
                     NAEARLRRALAIAQEQTALSWELRATMSLARLWQRQGRNSEAYELLAAAHAKFTEGFG
                     TSDLLQARSLLDSLAPAAQ"
     gene            complement(3135..>4559)
                     /locus_tag="VKQ27_08370"
     CDS             complement(3135..>4559)
                     /locus_tag="VKQ27_08370"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HLY88985.1"
                     /translation="SPSLQAVRLGGLALMGGMTIFGGAAEALLSRSMHRLRSLLPPEL
                     AGVVILLVAIGNGMVGYRYLLVSGGDQAEALHWAVATVTLLVTIALNVWGKGIARAAC
                     ALVGIIVGYGVALPLGLVPHDQLAQLADLPPLQLPHVGYFAWSFDAVLIAPFLIAALA
                     NTLKAAALLTATEKLTDADWVRPNLKKIGGGVLADGLTTMLSGAFCVFGVNISASSVG
                     LSEASGVASRVVAYVIGGIFVVMAFIPDLVRFFTLMPAAVIGATFIFTSCAIIKGGIE
                     TIASRMLDARRTLVVGLALMTGLAVEAFPRFFHAVPASIAPLVDSPLVLGTVVGFALN
                     AVFRIGTRRRAVLNVDPHAVDLGAIQSFMEGRGGAWGARRDVIARASFAAQQLIEVIA
                     HDCAPKGPIALSGSFDEFDLVLEARYDGELLALPEHRPTVDEIAHDEQGVRLLAGFLL
                     RHNADRSSSTRRGDTCVVQFQFHH"
BASE COUNT          842 a         1592 c         1364 g          761 t
ORIGIN      
        1 gcagtgcgtc cagaacgtcg cgcacatctc catcatcgtc gacaatagca acgatattca
       61 attctgctga cggcacttaa gtaactccaa accgtttccg cgatactgac cgagcgcgca
      121 ccacgaaagc gaatggcacc cgcgtgcgct cacgccctgt tgtcacgaaa ttctctctcc
      181 ggcccgatgg cgacgaggcg agggtgaggt tggggccctc ttccccccgt gtcgcacttc
      241 ggggattgac aaccccgcaa cctccacgac tgtctttgct tcacgcaagc tgcgtcccgc
      301 agcaccgagg ttacctttct cattgatcac gcagttcgga tgacgaccac cgccgaggag
      361 aacaaaccct acatcttcgg atcgttcgag ctggttcccg agcggcggca gctgctcaat
      421 agcggcaagc cggtcaatct cggcagccgc gcgttcgatg tgctgatcac gctcgtcaag
      481 tcccggggcg acaccgtcag caacgcgacg atcatggctc gggcctggcc tgcaacgacc
      541 gtcgatgaag cctccgtgcg cgtccacatc agtgcgcttc gcaagctgct tggtgacggt
      601 cgcggcacca atcgtttcat tgccaacgtt ccgggccgtg gctacgtgtt cgtcgcgccc
      661 gtcacacgcg gtcaggagct caccgccgca aagccgtcgg atcagttgcc gccacggagt
      721 ctgccaacgc cggcggcgga tattgtcggg cgggcgcccg tcatcgccag actggtggcg
      781 cttctgtcac ggcacaggtt cctcaccatc gtcggcatcg ggggaatcgg caagactacc
      841 gtcgcgctcg cggtgtccga ggccgccgcc gacgcttatc ccgacggaac ctggttcgtc
      901 ccgctcgcat cactgccgac gtccagcctt gtctcgagcg ccgtgggcgg cgcgctcggc
      961 ctggcaacga cgggacctga cacactgccc gtgttgacgg cgtcactgcg cgacaaacag
     1021 gcgctgatcg tgctcgacaa ctctgagcat gtgaccgatg gcgcggccga ggtggcagag
     1081 gcgatcctga aggcagcgcc gcgcgttagc atcctcgtca ccagccgaat tccattgcga
     1141 gccgaaggag aatggcgcca ccggctggaa cccttggaga cgccgtccca tcacgatgac
     1201 atcaccgctg ccgaggcgct ggcacatccc gctgtggaat tgttctacga acgcgctcgc
     1261 gcatcgagcg gcgaattcgc gattacggac gccaacgcgc ccctggtctg cgaggtctgc
     1321 cgcaggctcg acggcgtgcc gctcgcactg gaattggcgg cggcgcagat tggaacattg
     1381 ggcatcagcg ttctcgcccg cggcctgaac gatcgcttcg ccctgctgac cttgggtcat
     1441 cgcaccgcgc tgccgcatca gcaaaccctc cgtgcgacga tggactggag ctatgacttg
     1501 ctgactgcga cggaacaacg cgtgctgcag cgcctcgctg tgtttcgtgg cgacttcacc
     1561 acggaagcgg ccttcgccgt cgccggcgac gcggaactcg ccggcagcgc cgtgcttctc
     1621 cacatcgcga gccttgcgac gaagtctttg ctcgccaccg atatcagtgg cgacgtcact
     1681 tattttcgcc tgttcgaaac gacgcgcatc tacgcgattg aacgcctgaa ggagaccgcc
     1741 gccctggacg acgtcgcccg tcgtcacgcg gagtacttcc tcgcgctgct cgccaccctc
     1801 gacgaagaac gccaagcaca gccgcccgat gcacatacag ccctgttgcg ccgtcacggc
     1861 gatgagatcc acgcagcatt ggattgggct ttctccccaa cgggtgacac cggaatcggc
     1921 cttgccctga cgctcgcagc aatccccttg tggttcgaac tgttccagtt ggttgtggcc
     1981 cacgctcgcc tcgaacaggc cctggatcac gccgcgccgg attccgaaga cgaaatgcgg
     2041 ctccgtatcg cgatcggcca cgcgctctgg tacatcggct ccggcaccgc cgccattgaa
     2101 ccaaccttca cgcgcgtgct ggaaatcgcc gaacccatcg gcgcgaccac cgcacaaaca
     2161 caagcactct gggggttgtg ggcgtcatgc cgcggccgcg gcgattatcc cgccgcactc
     2221 accatggctc ggcgctttgc cgatgccgct gatcgcacag gcgacgtcag cgccatgcat
     2281 ctggccgatc gcatcctcgg tctgaccaac catttccttg gcgaccaacc cattgcgcgc
     2341 agcttcaccg aacgcgcact gcgccatccg gaacatctcg attcctcact tgggcttggt
     2401 tatcaggtcg agacgccggt tgcgatggcc gcgcaactag cgcgcatcct gtggctgcag
     2461 ggcttccccg accgcgccaa actcgcggcg accgatgcga tcgaagccgc ccggcagagt
     2521 gggcatccct tcgccatggt ctatgggctt gcgttcggga gtgcgtcggt ggcgctgtgg
     2581 accggcgata tcccagaggc cacgcgcgtg atcgaacggc tgatcgcccg taccgcaggc
     2641 aaccggcgca cggagcaatg ggtacgctat ttcgcaagcg tcctgaaatt gcgcggcggg
     2701 aacgatactg acgcgctcgt tgcatccttc gtagagccgc gggtggatct ctttcccgtg
     2761 cagctgtttg acgagctggt gacacagggc accattccag tgcctatgcc tggtcccgag
     2821 ccgtcccagg tgctttggaa cacagccgaa ctgctgcgca tcgacgccga actgctgctt
     2881 tggcacaacg gacccgatgc cgccgccaac gcggaagcaa ggctgcgccg cgcattggca
     2941 atcgcccagg aacagaccgc cctgtcgtgg gagctccgcg ccaccatgag cctcgcacgc
     3001 ctgtggcaac gccaggggcg aaactcggaa gcctatgagc tgctcgccgc ggcccacgcc
     3061 aaattcaccg aaggctttgg caccagcgat cttcttcagg cgcgcagcct gctcgacagt
     3121 ctcgcaccgg cggctcagtg atggaactgg aactgtacca cgcaggtgtc tccccgccgc
     3181 gtcgatgaag agcgatcggc attgtggcgc agcaggaatc ccgcaagcag ccgcacgcct
     3241 tgctcgtcgt gcgcgatctc gtcaaccgtc ggccggtgct ccggcagtgc cagcagctcg
     3301 ccgtcgtacc gcgcctccag caccagatcg aactcgtcga agctaccgga cagtgcgatc
     3361 ggtcctttgg gcgcgcagtc gtgggcgatc acctcgataa gttgctgcgc ggcgaagctc
     3421 gcgcgcgcga tcacgtcgcg ccgggcaccc cacgcaccgc cacgcccctc catgaagctc
     3481 tggatcgccc cgagatcgac cgcatgcgga tcgacgttca gcacggcgcg gcggcgcgtg
     3541 ccgattcgga atactgcgtt gagcgcgaag ccgacgaccg tgcccagcac cagcggcgaa
     3601 tcgaccagcg gcgcgatcga cgccggcacc gcatggaaga atcgcggaaa ggcttccacc
     3661 gccagccctg tcatcaatgc aagcccgacc accagcgtcc gcctcgcatc cagcatacgc
     3721 gaggcgatcg tctcgatgcc gcccttgatg atcgcacacg acgtgaagat gaacgtcgcc
     3781 ccgatcaccg ccgccggcat cagcgtgaaa aaacgcacaa gatcggggat gaacgccatc
     3841 accacgaaga tgccgccgat cacataggcg acgacacgtg acgccacgcc actcgcctcg
     3901 ctgaggccaa ccgacgacgc gctgatattg acgccgaaca cgcagaatgc gccggacagc
     3961 atggtggtga ggccatccgc cagcacgccg ccaccgatct tcttcaggtt ggggcgcacc
     4021 cagtcggcgt cggtcagctt ctcggtcgcg gtcagcagtg ccgccgcctt cagcgtgttg
     4081 gccagtgcgg cgatcaggaa tggcgcgatc agcaccgcgt cgaacgacca cgcgaagtag
     4141 ccgacatgcg gcagttgcag cggcggcagg tcggcaagtt gcgccagctg atcatgcggc
     4201 accaggccga gcggcagcgc gacgccgtag cccacgatga tgcccaccag cgcgcaggct
     4261 gcacgtgcga tgcccttgcc ccagacgttc agcgcgatcg tcaccagcag cgtcacggtc
     4321 gccaccgccc agtgcagcgc ttccgcctga tcgccgccag acaccagcag atagcgatat
     4381 ccgaccatgc cgttgccgat ggcgaccagc aggatcacca cgcctgccag ctccggcggc
     4441 aacaatgaac gaaggcgatg catggaacgc gagagcagtg cctcggccgc gccgccgaat
     4501 atcgtcattc cccccataag cgcgaggcca ccaagccgca ccgcttgcag cgacggact
//
LOCUS       DATGEM010000267         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_419753, whole genome shotgun sequence.
ACCESSION   DATGEM010000267 DATGEM010000000
VERSION     DATGEM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419753"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VKQ27_08375"
     CDS             <1..785
                     /locus_tag="VKQ27_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY88986.1"
                     /translation="LRRAHFLKEFDPMLRDELSAQMQADGVHVIVQTRIAKVERASTG
                     LRITQENGEVVEGLDGLLWAIGRKPNTDRLDLRATGVAMDTRGAILTDAYQATNVPGI
                     YAIGDVIGKLDLTPVAIAAGRRLADRVFGGMKERKLDYDNIATVLFTHSPIGTIGLTE
                     HEAVQAHGADNVKVYETRFTPMYHAFTRRKVKCSIKLIVAGATEKIVGCHVIGPGADE
                     IMQGFAVAIHMGATKRDFDDTVAIHPTVGEELVTLRTARPAG"
     gene            848..1327
                     /locus_tag="VKQ27_08380"
     CDS             848..1327
                     /locus_tag="VKQ27_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY88987.1"
                     /translation="MITLNVNGKIAIVDADPDTPLLWVLRDYLNLTGTKFGCGVAVCG
                     TCTVHIDSRAVRSCVMPVSACTGRKITTIEGLGVDGKPHRVQQAWIDHQVPQCGYCQS
                     GMIMAAAALLESKPHPSDQDIAEGITNICRCGTYPRVSKAIQALAESGTRPKHRARS"
     gene            1324..>2068
                     /locus_tag="VKQ27_08385"
     CDS             1324..>2068
                     /locus_tag="VKQ27_08385"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY88988.1"
                     /translation="MKARTMEDGKPANKWRRRLLITAGIASGALAIGAWRFYRERDKL
                     SPPATLKAGGNEAILTGWIRIGTDGTITVQVPRQEMGQGITTALPMLVAEEMDADLSR
                     VRFEQAPIDPLYANATMIGDGVPMRPDDNGWLATVMRHTQYKLGEALGVQATGGSTSM
                     RDGWITMRRAGAAAREMLVQVAANRFGVPAAQCSVDCSVVEHKPSGRRATFGELALEA
                     ASVPVPQNAPLKNPERFTLLGKSQVRFDIP"
BASE COUNT          421 a          680 c          644 g          323 t
ORIGIN      
        1 tgttgcggcg cgcgcacttc ctgaaggagt tcgatcccat gctgcgcgat gaattgtcgg
       61 cgcagatgca ggccgacggc gtccacgtta tcgtccagac caggatcgca aaagtggaaa
      121 gagcgtcaac cggactgcgc atcacgcagg agaacggcga agttgtggaa gggctcgacg
      181 gattgctctg ggcgatagga cgcaaaccga atacggatcg cctggacttg cgcgcaactg
      241 gagtcgccat ggacacgcgc ggcgctatac tgaccgatgc ctatcaagcg accaatgttc
      301 ccggcatcta tgccatcggc gacgtcatcg gcaaactgga cctgacaccg gtggcgatcg
      361 ccgctggccg gcgtctcgcc gaccgcgtgt tcggcggcat gaaggagcgc aaactcgact
      421 acgacaatat cgccacggtg ctgttcacgc attcgccgat cggcaccatc ggcctcacgg
      481 agcatgaggc agtgcaggcg cacggcgcgg acaatgtgaa agtctacgag acacgattca
      541 cgccgatgta tcacgccttc acccgccgca aggtgaaatg ctcgatcaag ctaatcgtcg
      601 ccggggccac ggagaaaatc gtcggttgcc acgtcatcgg ccccggcgcc gatgaaatca
      661 tgcaaggctt cgcggtcgcc atccatatgg gcgcgaccaa gcgcgacttc gacgatacag
      721 tcgcaatcca tcccacggtg ggcgaagaac tcgtcaccct tcgcaccgcc cgccccgccg
      781 gctgaccgcg gctggacaag cgcacgcgag cgacgtcccg cattcaccca tgtccgcgga
      841 accagccatg atcacactca acgtaaacgg caagatcgcc atcgtcgatg ccgacccgga
      901 tacgcccctg ctgtgggtgc tgcgcgacta tctgaatctc accggtacga aattcggctg
      961 cggcgtggca gtctgcggca cctgcaccgt gcatatcgac agcagggcgg tgcggtcctg
     1021 cgtgatgccg gtctccgcct gcacgggccg gaagatcacc accattgagg ggcttggtgt
     1081 cgacggcaag ccgcatcgcg tgcagcaagc atggatcgac catcaggttc cgcagtgcgg
     1141 ctattgccag agcggaatga tcatggcggc ggccgcactg ctcgaatcga agcctcatcc
     1201 gtcggaccag gacattgccg agggcatcac caatatctgt cgctgcggca cctatcctcg
     1261 tgtcagcaag gcgattcagg cgctggccga atccggaacc cgccccaagc atagagcgcg
     1321 atcatgaagg cgcgaaccat ggaagacgga aaaccggcga acaagtggcg ccgccgcttg
     1381 ctcatcaccg cgggcatcgc cagcggtgcg ctcgcgatcg gcgcatggcg cttttaccgc
     1441 gaacgcgaca agctctcgcc tcccgccacg ctcaaggctg gcggcaacga agcgattctg
     1501 accggttgga tccgcatcgg caccgatggc acgatcacgg tccaggtccc gcgccaggaa
     1561 atgggccagg gcatcacgac cgcgctgccg atgctggtcg ccgaggaaat ggatgccgat
     1621 ctgtcccgtg tacgcttcga gcaggcgccg atcgatccgc tctacgcgaa cgcgaccatg
     1681 atcggcgacg gcgtaccgat gcgccccgac gacaacggct ggctcgccac cgtgatgcgc
     1741 cacacgcaat acaaactcgg cgaagcgctc ggcgtgcagg ccaccggcgg ttccaccagc
     1801 atgcgcgacg gctggatcac gatgcggcgg gccggtgcgg cagcccggga gatgctcgtg
     1861 caggtggcgg caaaccgctt cggcgtcccg gctgcacagt gcagcgtcga ttgcagcgtg
     1921 gtcgaacaca agccgagcgg aagacgcgcc acgttcggcg agctcgcgct ggaagcggca
     1981 tccgtccccg tgccgcagaa tgcgccgctc aagaaccctg agcgcttcac gctgctcgga
     2041 aaatcgcagg tccgcttcga cattcccg
//
LOCUS       DATGEM010000268         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_645576, whole genome shotgun sequence.
ACCESSION   DATGEM010000268 DATGEM010000000
VERSION     DATGEM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645576"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VKQ27_08390"
     CDS             complement(<1..336)
                     /locus_tag="VKQ27_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLY88989.1"
                     /translation="MERKDVEFPADGGVTLRGWLYLPSESNGRLPAITMAHGYAGIRQ
                     HGLERFARAFADAGFVVLLHDHRGFGASDGAIRHDIDPWQQIADWRYAISFLESLPNV
                     DPERIGLWGT"
     gene            429..1631
                     /locus_tag="VKQ27_08395"
     CDS             429..1631
                     /locus_tag="VKQ27_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY88990.1"
                     /translation="MTAMPEQKTEADAIAAETTATRILFIHGFLDGASIWDAVRERLA
                     SVHLKAEAIDLAGMGGTDAPADSISLDGYADDVVAHIRNGAAPVILVGQSMGSQIADL
                     VATRHPEMVAGLILLTPVPLAGARLPREVVAPFRDSGAKPDVQRSLREQLSFVLDQEQ
                     ADALTALGLQVKPDVVARLVDVWNEGHAAGREVARYEGPVVVVRGAADPFVNESMVGE
                     TLARYPAATSIQIDDAGHWAHVEQPAAVAAALGGFVAGLRGASQKDKAGDWQQAFADR
                     KASSFADAFAEDVELDASAMLAPVRGRDLVKKVMAVASRQYRELTFTDQATAGSKQYL
                     EWRATGHDGVAYRGVTILTRDVQGAMRQIAIHHRPLGAAMHFSRALADGLRGEIDAAH
                     FLESLESF"
     gene            1646..2371
                     /locus_tag="VKQ27_08400"
     CDS             1646..2371
                     /locus_tag="VKQ27_08400"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLY88991.1"
                     /translation="MIIDANELDAHHAYKLLIATVLPRPIGWLSTLSTDGVPNIAPFS
                     FFTVVGRKPPILSISMQPRSDDGTLKDSFINIRDTGEFVAHLVTLEMADKMHQTARDL
                     PPETDEFAEVGLERLPSEVVKPWRIAGPPIAMECKVDRIIPVGEFHDHVVWGRVVRFH
                     IRDDLYLENGRVDTAAFTPIGRLAAEYTAVDNVFTTPLEAGILEARRGRRMHRLDGLP
                     ADFAAVAQKNWSPSGAVTEKVEA"
     gene            2368..2853
                     /locus_tag="VKQ27_08405"
     CDS             2368..2853
                     /locus_tag="VKQ27_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLY88992.1"
                     /translation="MTEHLELPVLCNCARIRRASRVMTRYYDSVLAPSGLGINQFTLL
                     GYLHARGPMPLNRLSDLLAMDRATIGHNVRPLERDGLLRLVQSSQDRRVKMAELTAKG
                     LAKLDEARPLWFDAQKNFENVVGAEQAETIRAAMDAISDHRTNALAPLGVSGGSGATL
                     G"
     gene            complement(2890..>3171)
                     /locus_tag="VKQ27_08410"
     CDS             complement(2890..>3171)
                     /locus_tag="VKQ27_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88993.1"
                     /translation="FGEPTEVLELVDLPKPSQPAAGEVLVGVEYAPINMNDFYLMQGA
                     FPIRPSLPRIVGNVRLTQHALTSAMTFARHRRARRQDAASRCLLRSTAR"
BASE COUNT          600 a          950 c         1036 g          585 t
ORIGIN      
        1 cgtcccccac aggccgatcc gctcgggatc gacattcggc aggctctcca ggaaggagat
       61 ggcgtagcgc cagtcggcaa tctgctgcca ggggtcgata tcatgtcgga tcgcgccgtc
      121 gctggcgccg aagccgcgat ggtcgtgcag cagcacaacg aagcccgcgt cggcaaaggc
      181 gcgcgcaaat cgctcaaggc cgtgctggcg gatgccggca tagccatgag ccatggtgat
      241 cgccggtaac cggccgttcg actcgctggg aagataaagc cagccgcgta gggtgacgcc
      301 gccatcggcc gggaattcaa cgtctttgcg ttccatatca gcatctcttg caatataggg
      361 gcatatgaga ctatatagag aaaacaccgg tggtccgcaa ccatcgatcg caaggcagga
      421 tagatatgat gaccgctatg cccgagcaaa agaccgaggc ggatgcgatc gcggccgaaa
      481 caaccgcgac gcggatcctc ttcatccacg gatttctgga cggggcgtcg atatgggacg
      541 ctgtgcgaga gaggctcgcg tctgtgcacc tgaaggccga ggcgatcgac cttgctggca
      601 tgggcggtac agatgcgcca gccgattcca tctcgcttga cggatacgcc gatgacgtcg
      661 tggcccatat ccgcaatggc gcggcgcccg tgatcctggt cggacagagc atggggtccc
      721 agatcgcgga tctcgtcgcc acgcgccatc cggagatggt ggccggactc atccttctga
      781 cgcccgttcc tctggcggga gctcgactgc cgcgtgaagt cgtagcgccg ttcagggact
      841 ccggcgcgaa gcccgacgta cagaggagtc tgcgcgagca actgtccttc gttctcgatc
      901 aggagcaggc cgatgcgctg acggcactcg gactgcaggt gaagcctgac gtcgtcgcgc
      961 gtcttgtcga tgtctggaat gaaggccatg ctgcggggcg cgaggtcgct cgttatgagg
     1021 ggcctgtcgt ggttgtccgc ggtgccgccg atccgttcgt caacgagtcg atggtcggcg
     1081 agacactggc gcgctacccc gctgcaacat ccatccaaat cgacgatgcc ggtcattggg
     1141 ctcatgtcga gcagccggcg gcggtggcag cggcactcgg cggctttgtc gcagggcttc
     1201 gcggagccag ccagaaggat aaggctggcg attggcagca ggctttcgcg gaccgcaagg
     1261 catcgtcctt tgccgatgcg ttcgccgagg atgttgaact ggatgcgagc gcgatgctgg
     1321 ccccggttcg ggggcgtgac ctcgtcaaga aggtcatggc ggtagcaagc cgtcagtacc
     1381 gcgagctcac tttcaccgat caggcgacgg ctggttccaa gcaatatctt gaatggcggg
     1441 cgaccggcca tgatggcgtc gcatatcgcg gcgttaccat tctgacgcgc gacgttcagg
     1501 gtgcgatgcg tcagatcgcg atccaccacc ggccactcgg cgcggccatg catttctccc
     1561 gtgcgctggc cgacggtttg cgcggcgaaa tcgatgccgc gcacttcctt gaatccctcg
     1621 agtccttctg agcaggagag gtaccatgat cattgatgct aacgaactcg acgcccatca
     1681 tgcctataag ctgctcatag cgaccgtgct gccccggccg atcggctggc tgagtaccct
     1741 gtcgacggat ggcgtcccga acatcgcgcc tttctcgttt ttcactgtcg tcggccgcaa
     1801 gccgccaatc ctctcgatct ccatgcagcc ccgctccgac gacgggacgc tcaaggacag
     1861 ctttataaat atccgggata cgggcgagtt cgtcgcccat ctggtgacgc tggagatggc
     1921 ggacaagatg catcagacgg cgagggactt accgccggag actgacgaat tcgccgaagt
     1981 cgggctggaa cgcttgccga gcgaggtggt gaagccctgg cgaatagccg gtccgcccat
     2041 tgcaatggag tgtaaggtcg accgcatcat tccggtcggc gagttccacg accatgtcgt
     2101 gtggggccgc gtcgtgcgct tccacatccg cgacgatctc tatctggaga atggtcgcgt
     2161 cgacacggcc gcgttcacgc ccataggcag gctcgccgcg gaatatacgg cggtcgacaa
     2221 cgtgttcacg acgccactag aggcgggcat cctggaggcg cgtcggggcc gccgcatgca
     2281 tcgcctggac ggcctgcctg cggacttcgc cgctgttgcc cagaagaact ggtcgccgtc
     2341 gggggccgtg acggagaagg ttgaggcatg accgaacatc tcgaattgcc ggtgctgtgc
     2401 aattgcgcgc ggatccgtcg agcatcgcgt gtcatgacgc gctattacga cagcgtgctg
     2461 gcgccatcgg ggctcgggat caaccagttc accttgcttg gctacctcca cgcccgtggt
     2521 cctatgccgc tgaacaggct gtccgaccta ctggccatgg atcgggccac gattggccat
     2581 aatgtgcgcc ctcttgagcg agacgggctg ctgaggctgg tgcaaagttc gcaggatcgc
     2641 agggtcaaga tggcagaact gacagccaag gggctggcca aactcgatga agcccggccg
     2701 ctctggttcg atgcccagaa gaatttcgag aatgtcgtcg gtgccgagca ggcggagacg
     2761 atccgtgccg ctatggacgc gatatcggat caccggacca atgcccttgc ccccctcggt
     2821 gtgagcgggg ggagcggagc gacactgggg taacagccat caagggtaac atccggcggg
     2881 acggagcgat cagcgcgccg tcgaacgcaa caaacaacgg gatgccgcat cctgtcgcct
     2941 cgcccggcgg tgccgtgcga aggtcattgc cgacgtgagg gcgtgctgcg ttaggcggac
     3001 attgccgacg atcctgggta gtgaaggtcg aattgggaaa gcgccttgca tgaggtaaaa
     3061 gtcgttcatg ttgatcgggg catattctac accgaccagc acctcgcccg cagccggctg
     3121 tgacggcttc ggcaggtcga caagttcgag aacctctgtc ggttcaccaa a
//
LOCUS       DATGEM010000269         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_97162, whole genome shotgun sequence.
ACCESSION   DATGEM010000269 DATGEM010000000
VERSION     DATGEM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97162"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VKQ27_08415"
     CDS             <1..369
                     /locus_tag="VKQ27_08415"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLY88994.1"
                     /translation="METLRSRCRLRDGAIHTDCALKQVTALIYLNDGWLDQCGRLRLP
                     SGPDDIEDMIAEVPPLAGTLLAFRRSDNSWHGHKPFAGVRRAIMLNWMTDASAARRER
                     TRHAFSARVKRMANPMHGAG"
     gene            369..1013
                     /locus_tag="VKQ27_08420"
     CDS             369..1013
                     /locus_tag="VKQ27_08420"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLY88995.1"
                     /translation="MLGYVRRTLLTARQCDQPFCHWLLDDARPPFLADALAALPFAPP
                     LIGDTEGKRETDNGTRRLVTPVERGASPACASLADLLQDSATTALLGSVCGRSLAGSH
                     LRIEYCQDTDGFWLEPHTDIAAKRFTMLIYLSTHADAAEWGTDLFDADLQPAGRSSGQ
                     FNSGLMFLPANDTWHGFARRPIAGVRRSIIVNYVTPDWRSRHELAFPARPVSDV"
     gene            complement(1033..1815)
                     /locus_tag="VKQ27_08425"
     CDS             complement(1033..1815)
                     /locus_tag="VKQ27_08425"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HLY88996.1"
                     /translation="MVEFILPSNSRIGRGKTWKAPSGAKRIREFRIYRWSPDDGQNPH
                     TDTYEVDLDTCGPMVLDALIKIKNEIDPTLTFRRSCREGICGSCAMNIDGGNTLACLK
                     PIDEVKGTVRISPLPHMPVVKDLVPDLSQVYAQLRSIEPWLQADSPPPPDGERLQSHE
                     ERAKLDGLWECILCFCCSTACPSYWWNGDRYLGPAVLLQAYRWIADSRDEHTGERLDA
                     LEDPFKLYRCHTIMNCTDTCPKGLNPAKAIGSIKQLIVERQV"
     gene            complement(1817..>2323)
                     /locus_tag="VKQ27_08430"
     CDS             complement(1817..>2323)
                     /locus_tag="VKQ27_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629438.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HLY88997.1"
                     /translation="DASQPPLPPKAGEASLDRFDRTRHAKGGTRVANLRLDMQRTMQS
                     HAAVFRSSKMLAEGVNAMRRVWRGMEDMSVSDRSLIWNSDLVEALELQNLMGCAMTTM
                     AGAEARHESRGAHAHDDYPERDDANWMKHTLAWCDDRGDVALDYRPVKMQTLTNEVSV
                     IPPKQRVY"
BASE COUNT          387 a          783 c          719 g          434 t
ORIGIN      
        1 atggagacac tacgttctcg ctgtcgcttg cgcgatggcg ccatccacac cgactgcgcg
       61 ctgaagcagg tgaccgcgct gatctacctg aatgacggct ggctcgatca gtgcggccgg
      121 ttgcgcctgc cgagcggtcc cgacgacatc gaggatatga ttgccgaggt cccgcccctg
      181 gcaggcacgc tgctggcatt ccgccgctca gacaattcat ggcacggcca caagccgttc
      241 gcgggcgtgc ggcgcgccat catgctgaat tggatgactg acgcctccgc agcgcgtcgc
      301 gagagaactc gccacgcctt ctcggcgcgt gtgaagcgca tggcgaaccc aatgcacggt
      361 gcgggctgat gctgggctat gtccgccgaa cgctgctgac cgcgcgacaa tgcgatcaac
      421 ccttctgcca ctggctgctg gacgacgcgc ggccgccgtt tctcgcagat gcactcgccg
      481 cgctgccgtt cgcgccgccg ctcattggcg ataccgaggg caagcgcgag accgacaacg
      541 ggacaagacg cttggtcacg cctgtcgagc gcggtgcatc cccggcctgc gcctcgctgg
      601 cggacctgct gcaggacagc gcgacgaccg cgctgctcgg ctctgtctgc ggccgatcgc
      661 tcgccggttc gcatctacgc atcgagtatt gccaggatac cgacgggttc tggctggaac
      721 cgcacaccga catcgcggcc aagcggttca ccatgctcat ttacctctcg acgcacgctg
      781 atgccgcaga gtggggcacc gacctgttcg acgcggactt gcagccagca ggtcgttcat
      841 ccgggcagtt caacagcggc ctgatgttcc tgccggcaaa tgacacgtgg catggatttg
      901 cccgccggcc tatcgccggg gtgcggcgat cgatcatcgt caactacgta acgccggatt
      961 ggcggtcgcg ccacgagttg gcgttccccg cacgccccgt cagcgatgtg tgactgagaa
     1021 gagccggcct tcctacacct gccgctcgac aatcagctgc ttaatcgagc cgatcgcctt
     1081 cgccggattc agccccttcg ggcatgtatc cgtgcagttc atgatcgtgt ggcagcgata
     1141 cagcttgaag ggatcctcca gcgcatccag ccgctcgccc gtgtgctcgt cgcgagagtc
     1201 ggcgatccag cgataggctt gcagcaacac cgctggtccc agatagcggt cgccattcca
     1261 ccagtagctc gggcacgcgg tggaacagca gaagcacagg atgcactccc acagaccgtc
     1321 cagcttggcc cgctcctcat ggctttgcag ccgctcgcca tcgggcggcg gagggctgtc
     1381 cgcctgcagc catggctcga tgctgcgaag ctgcgcatag acctgcgaca gatcaggcac
     1441 caggtccttg accaccggca tgtgcggcag aggcgaaatc cgcaccgtgc ccttcacttc
     1501 gtcgatcggc tttaggcagg ccagcgtatt gccgccatcg atgttcatcg cgcaggagcc
     1561 gcagatgccc tcgcggcagg agcgacgaaa tgtcagcgtc ggatcgatct cgttcttgat
     1621 cttgatcagc gcgtccagga ccatcggccc gcacgtatcc agatccacct cgtaggtgtc
     1681 ggtatgcggg ttctgcccat catctggcga ccagcgatag atgcggaact cgcgtatccg
     1741 cttggcaccg gagggcgcct tccaggtctt gccacgaccg atgcggctgt tggagggcag
     1801 gatgaattcg accatctcag tatacccgct gcttcggcgg gatcaccgag acttcattgg
     1861 tcagcgtctg catcttcacc ggccgataat ccaacgcaac gtcgccgcgg tcatcgcacc
     1921 aggcgagggt gtgcttcatc cagttcgcgt cgtcgcgttc gggataatcg tcgtgtgcgt
     1981 gggcgccacg gctctcgtgg cgcgcctcgg cgccagccat cgtcgtcatc gcgcagccca
     2041 tcaggttctg cagttcaagc gcctcgacca ggtcgctgtt ccagatcagg ctgcgatccg
     2101 acaccgacat gtcttccatg ccgcgccaca cgcgacgcat ggcattgacg ccctcggcca
     2161 gcatcttgct gctacggaag accgcggcgt ggctctgcat cgtgcgctgc atgtcgagcc
     2221 gcagattggc cacccgtgtg ccacctttgg cgtggcgcgt gcggtcgaag cggtccagcg
     2281 aggcctcccc cgccttgggc ggcagcggcg gctggctggc gtc
//
LOCUS       DATGEM010000270         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_356318, whole genome shotgun sequence.
ACCESSION   DATGEM010000270 DATGEM010000000
VERSION     DATGEM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356318"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VKQ27_08435"
     CDS             <1..774
                     /locus_tag="VKQ27_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88998.1"
                     /translation="LHDAHRLTRSGKGTFDRTMAGVRLLQRHNAEFHVITVLTAGSLD
                     NPDALVRFYLDAGIDQVCFNLEESEGDHTSELFTAADVRTRFATFLDRFWRAARQTGR
                     FSYIREIDGMLPRILRPEEAVMGNEQVAPFGMLNVACNGDVSSFSPELLGLRNADYSD
                     FIVGNVHTGPLEQMLASAPMQRMAADIDRGVALCRSTCEYFSVCGGGAPINKLTENGS
                     FATARTRFCELTQMVPTDLILDALERMELNGQSALAAAT"
     gene            789..1526
                     /locus_tag="VKQ27_08440"
     CDS             789..1526
                     /locus_tag="VKQ27_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY88999.1"
                     /translation="MSQSNRMFRVALVALGALAATTGIALAQSAPAKPTSDNASAPAS
                     GAQDMAQSPSSSEDTTTSFPVFAVTSVEILHSTHNPSLSVIAVRGLTSSEGWDQGTLV
                     PLTSGNPADGVLDLVLVANAPPDAAAPGTYAPIEAVLPVPAMPFKAVRVRSASNTVTL
                     KDLNGYTEVKAPENPCGPCVGKRFVAKGASAPAGVEAGEIVREEDLPPDTRVLMPTDG
                     IKDMRTNPNRLTVIVGEDGRIVDAIWE"
     gene            1510..1977
                     /locus_tag="VKQ27_08445"
     CDS             1510..1977
                     /locus_tag="VKQ27_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89000.1"
                     /translation="MRSGNDTAFTAIALLIAMHAHAETPAAFCQRVGTDDTTRPIPEV
                     LVPEVNTAFGTSMPARMVMDTTVYRCAAGHVLACTVGANLPCGKADASRTPGEGLVQW
                     CRANPDAASIPAAVTGHDTLYEWSCHAGTPGIVRQTLHADPRGFVAEFWKRLP"
     gene            2231..3172
                     /locus_tag="VKQ27_08450"
     CDS             2231..3172
                     /locus_tag="VKQ27_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89001.1"
                     /translation="MALDSLDALLHFAPDYDSAIPYMQRTREYYGAIGYTTPYRWAHY
                     VEAPFTPFRTPLAKARVTIITTAAPYRADKGDQGPGAPYNGGAKFYEPYSGDTSQTHD
                     LRISHIGYDRVHTTATDSNTWFPLPALHRAVAAGRVGAITPRFHGAPTNRSHRVTIET
                     DAPEILRRCQEDGTDAALLVPNCPVCHQTSSLVARHLEANGIPTVVMGCAKDIVEHAA
                     VARFLFSDFPLGNSAGKPHDVASQDQTLELALRVLETAPGARTTMQSPIRWSENAEWK
                     LDYLNLDRMPPEELARRRREFDEQKAIARTLRVEKAA"
     gene            3356..>3833
                     /locus_tag="VKQ27_08455"
     CDS             3356..>3833
                     /locus_tag="VKQ27_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY89002.1"
                     /translation="MTRPFANVRILDFTQVLAGPYGSYQLALLGADVIKVERREGEDM
                     RRTPLSREWADRGMAPSWLAINGNKRSLTLDLQKPEAIEIVKRLAAEADVVMENFRPG
                     VMDKLGIGYAALSAINPRLIYCAISGFGQTGPERSGAGYDGKIQAMSGIMAITGHQE"
BASE COUNT          707 a         1355 c         1134 g          637 t
ORIGIN      
        1 ctgcatgacg cccatcgcct cacgcgcagc ggcaaaggta cattcgaccg gaccatggcc
       61 ggcgtccgcc tgctccagcg ccataacgcg gaattccacg tcatcaccgt gctgactgcc
      121 ggcagcctcg acaatcccga cgccctcgtg cgtttctacc ttgatgccgg tatcgaccag
      181 gtctgcttca acctcgagga gtcggaaggc gatcacacct cggaactgtt taccgctgcc
      241 gacgtgcgta cgcgcttcgc cactttcctc gatcggttct ggcgtgcggc ccgtcagaca
      301 ggccgcttca gctacatccg cgagatcgac ggcatgctgc ctcgcatcct ccgtcccgag
      361 gaagccgtga tgggcaatga gcaggtagct ccgttcggta tgctgaatgt tgcctgcaat
      421 ggcgacgtat ccagcttctc gccggaactg ctcggcctcc gcaacgccga ctacagcgac
      481 ttcatcgtcg gcaatgtcca cactggcccg ctggaacaga tgctggcaag cgcgccgatg
      541 cagcgcatgg cggcggacat cgatcgcggt gtggcattgt gccggagcac ctgcgaatac
      601 ttctcggtct gcggcggtgg cgctccaatc aacaagctga ccgagaatgg cagcttcgct
      661 actgctcgca cgcgtttctg cgagttgact cagatggtcc ccaccgacct catcctggat
      721 gcgctcgaac gcatggaact gaacggccaa tccgctctgg ccgccgcaac ctaaagctgg
      781 aggacgcaat gtcacagagc aaccgaatgt ttcgggtcgc ccttgtcgca ctgggtgcgc
      841 ttgctgcaac taccggcatt gccctggcgc agagtgcacc cgcgaagcca acttccgata
      901 atgcctcggc gcccgcgtcc ggggcacagg atatggcgca gtcgccatcc agctctgagg
      961 ataccaccac aagcttcccg gtcttcgcgg tgaccagcgt ggaaatcctt cacagcacac
     1021 acaatccatc tctctcggtg attgcggtgc gcggcctgac gagttcagag ggttgggatc
     1081 agggcacatt ggtgccgctg accagcggca acccggctga cggcgtgctg gacctcgttc
     1141 tggttgccaa cgcaccgccg gacgcagcag cccctggaac ctacgcgccg atagaggcgg
     1201 tcctgcctgt gccggcaatg cccttcaagg cggttcgtgt ccgcagcgcc agcaacacgg
     1261 tgacgttgaa agacctgaac ggttataccg aggtgaaggc gcccgaaaac ccctgcggcc
     1321 cctgcgtcgg caagcgcttc gtcgcgaaag gcgcctcggc gccagctggc gtggaggccg
     1381 gcgagattgt gcgtgaggaa gatctgccgc cggatacgcg agtgctgatg ccaaccgacg
     1441 gcatcaagga catgcgcacc aatccgaacc gcctgacggt gatcgtgggc gaggacggcc
     1501 gtatcgtcga tgcgatctgg gaatgacacc gctttcaccg cgatcgcgtt gctgatcgcg
     1561 atgcacgccc acgccgaaac gccggcggca ttctgccagc gcgtcggcac tgacgacacg
     1621 acgcggccaa tcccggaagt tctggtcccc gaggtgaata ccgcattcgg aaccagcatg
     1681 ccggcgcgca tggtgatgga tacaaccgta tatcgctgtg ccgccggcca tgtgctggca
     1741 tgtaccgtcg gcgccaacct cccgtgcggc aaggcggacg ccagccggac acctggtgag
     1801 ggtctggttc aatggtgccg cgccaatccc gatgccgcgt ccatccctgc agccgtgacc
     1861 gggcacgata cgctgtatga atggtcctgc catgccggta cccccggcat cgtccggcag
     1921 acgctccacg ctgatccgcg cggcttcgtc gccgagttct ggaagcggtt gccgtaaact
     1981 caggcgcctg cccgcttcac ggtttcttaa gcgtttaaca cggggaaggc cgaggactac
     2041 ggagcgccac aggagtgcgc cgctttgtcg tctgaccacg gactcccgtg gcgctccatg
     2101 gtcctcggcc ttctccgtgt tgagaagctg gccggggaac cggcgcggac ggtgccgcta
     2161 agccccggca agcctctgtc caatctcccc caaccgcgcc atgctgcgga caaagcaccg
     2221 gagacaccct atggccctgg acagcctcga cgcgctgcta cacttcgccc ccgactacga
     2281 ctccgccatc ccctacatgc agcgcacccg cgagtattac ggggcgatcg gctataccac
     2341 gccgtaccgc tgggcgcatt acgtcgaggc ccccttcacc ccgtttcgca cgcccctggc
     2401 gaaggcccgc gtcaccatca tcaccaccgc cgccccctat cgcgcggaca aaggcgacca
     2461 aggccctggc gcgccctaca acggcggtgc caagttctac gagccctact ccggcgatac
     2521 ctcgcagacg cacgaccttc gcatctcaca catcggctac gaccgggtcc acaccacagc
     2581 gaccgacagc aatacctggt tcccccttcc cgccctccac cgcgccgttg ccgctggccg
     2641 ggtcggtgcc atcacgcccc gcttccacgg tgcccccacc aaccgcagcc accgcgtcac
     2701 gatcgagacc gacgcgccgg aaatcctccg ccgctgccag gaggacggga ccgatgccgc
     2761 gctgctcgtc cccaactgcc cggtctgcca ccagaccagc agcctcgtcg cccgtcacct
     2821 tgaggcgaac ggcatcccca ccgtcgtgat gggctgcgcc aaggacatcg tcgaacacgc
     2881 cgccgtcgcg cgcttcctgt tcagcgactt cccgctcggc aacagcgccg gcaagccgca
     2941 cgacgtggcc tcgcaggacc agacactgga actcgcactg cgtgtcctgg aaaccgcgcc
     3001 cggggcccgc accacgatgc aatcgccgat ccgctggagc gagaatgccg agtggaagct
     3061 cgattacctg aacctcgatc gcatgccgcc ggaggaactc gcccggcgcc gccgcgaatt
     3121 cgacgagcag aaggcgatcg cccgcaccct ccgtgtcgaa aaggccgcct gatcacttga
     3181 agctcgcggc gcgccaccct ggagcgcgcc ttcgccgcca gacgcaaccc gcggatgtgg
     3241 ggcgctagta ctatatcctc tggcgcatcg ccgaaggcgc ctcgtcgcgg acagccggcg
     3301 ccaggctcat gctcgaagtc aaccgcctac gcttcattcc acaagagaac cagcaatgac
     3361 ccgccccttc gccaatgtcc gtattcttga cttcacccag gtgctcgccg gcccctatgg
     3421 cagctaccag cttgcattgc tcggcgctga cgtgatcaag gtggaacgcc gcgagggcga
     3481 ggacatgcgc cgcacgccgc tctctcgtga atgggccgat cgcggcatgg cgccatcgtg
     3541 gctggcgatc aacggcaaca agcgcagcct gacgctcgac ctgcagaagc cggaagcgat
     3601 cgagatcgtc aaacggctcg ccgccgaagc cgatgtggtg atggagaatt ttcgccccgg
     3661 cgtgatggat aagctcggga tcggctacgc cgcgctctcc gcgatcaatc cgaggctgat
     3721 ctattgcgcg atctcgggct tcggccagac cggtccggaa cgatcaggcg caggttacga
     3781 cggcaagatc caggcgatgt cgggcatcat ggcgatcaca ggccaccagg aga
//
LOCUS       DATGEM010000271        10442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1040566, whole genome shotgun sequence.
ACCESSION   DATGEM010000271 DATGEM010000000
VERSION     DATGEM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040566"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VKQ27_08460"
     CDS             <1..223
                     /locus_tag="VKQ27_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HLY89003.1"
                     /translation="AETDDLMQNAQAKRVRKKADWIVANDVSPETGIMGGEENAVHLI
                     TAEGVEDWPRLAKQEVARRLAERIAVALR"
     gene            complement(331..1281)
                     /locus_tag="VKQ27_08465"
     CDS             complement(331..1281)
                     /locus_tag="VKQ27_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89004.1"
                     /translation="MARNIAIGVGLMEFPFSSTDGFWRWVDMCEAGGIDSIWQTDRIV
                     SRQPILECMTALAAIAGRTRRIKFGVNVVSLALRDPVLLAKQCATIDVLSQGRLLPGF
                     GIGSPQGPEWSTLHIDTATRGRRTDEALEIIARLWSQDSVDFEGRHNHLTGASISPKP
                     VQPDLPMWIGGSSAAAVRRTARYGTGWQAGAETPADVGRVIAEIKAAVVEAGRCIDED
                     HYGAAFAYHFGKADDPGVAQVMEAYRKRTGRDPERHFAIGDAALMLDRIADYVEAGAS
                     KFILRPAARGDEGMLDQTRKLIEQVLPMVATRWPRQKKAA"
     gene            complement(1293..2042)
                     /locus_tag="VKQ27_08470"
     CDS             complement(1293..2042)
                     /locus_tag="VKQ27_08470"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HLY89005.1"
                     /translation="MDPSIAELRRMIEEAQRVVFFTGAGISTESGIPDFRSPGGIWTK
                     MKPIDFSEFMSSAESRRETWRRRFATDETIRSAEPNRGHRAVAALIDRGKASAVITQN
                     IDGLHQASGVPANRVIELHGNTTYAHCLDCAQRYEIIDVREEFERTGEPPNCNACGGF
                     VKTATVSFGQSMPPAAMIRAEEETLAADLFISIGSSLVVYPAAGFPEMAARNGTRLVI
                     LNREPTGLDPYADLVLHREIGATLGEAVGNN"
     gene            2155..3372
                     /locus_tag="VKQ27_08475"
     CDS             2155..3372
                     /locus_tag="VKQ27_08475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89006.1"
                     /translation="MSEITVARPIRRAWLIDSADFWRLWSVGLVVFTVRWVETVAVGV
                     FVYERTGSAFLVAMMTMLRLLPMGLFGAFLGAMAERIERRTSLLIVVAGMVVTSVTLA
                     TLAYLNQLEVWHLAVASFVNGMGWATDNPVRRVMIGEAVGASQMGTAMSIDVGANNAS
                     RMIGPTIGGLLLATMGIAGVFTLSVVLYVIALFAAFRVRYRNSFPSAAGESVLARIAE
                     GLRLVRNDKRLVGTLTVTVIYNVFGWPFTSMVPVIGKDHLQLGPEGIGALSSMDGIGA
                     FVGAVLMAMLCRPAFYRYAYVGGVLLYMVMLTVFALVPMPALAASALLFTGLGGAGFS
                     IMQATLVYLSAPPEMRSRMLGVLSVCIGVGPVGFVALGWLADTIGAQWATAATGITGL
                     VMMLLARPLWRGI"
     gene            complement(3355..3876)
                     /locus_tag="VKQ27_08480"
     CDS             complement(3355..3876)
                     /locus_tag="VKQ27_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89007.1"
                     /translation="MSHMVRELGQDDVAAFRRVRLDALRLHPEAFTASYEDEVQLSEA
                     QFAERLAAPGLTRFGGFAGDELVGLVGLNLRTGPKERHKAGLFSMYVAAGHRRSGLAR
                     DLVSAVIDHARGAGALVLQLFVTAGNAPAQRLYQRMGFVPYGTERRAIRIGDVFYDEE
                     LMALDLDRSDAAP"
     gene            complement(3883..4416)
                     /locus_tag="VKQ27_08485"
     CDS             complement(3883..4416)
                     /locus_tag="VKQ27_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89008.1"
                     /translation="MDIAKRFPQLPENVLRRMIDDMCSLLPPPEPDTPENRERFRQAA
                     INEFIALDPFDIFDAMAAKDIVVCEAMAWDCSRNSAKHGVELKLIGGWESLARKNLSL
                     AQSMRRDIERRRKKRLKEAQKKAPPPKREPSPLVLTPTGVAFRDLPGRLSLTSEQRTA
                     RIRALRLHLVEAPPTVH"
     gene            complement(4481..5398)
                     /locus_tag="VKQ27_08490"
     CDS             complement(4481..5398)
                     /locus_tag="VKQ27_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89009.1"
                     /translation="MPHDLRFFTMILPNQPWPELLRRYRHLEELGFDIAAFADHFADW
                     TGGKGIWFECFTLLTAVASRTSRIRLGAYVAQIPLRNPALLAHQALTLDHVSNGRLEL
                     GLGLGLPTDPSYAMMGLPNWSNRERVARFPEYVQLVDRLLRDDVTTFHGQHYQADSAV
                     TQPRPIQQPRPPIVIAAMGPTMLRHAAQYADTWNSISFTDSFDAQIKETRERIRLVDE
                     HCLKIGRDPATLRRSYLMLDVQARRTGGPVNYYASNDAFADMVRQVLALGVSEIGLYY
                     PLRDDDTARFETIATQIIPELKREYAGRN"
     gene            complement(5924..6772)
                     /gene="nadC"
                     /locus_tag="VKQ27_08495"
     CDS             complement(5924..6772)
                     /gene="nadC"
                     /locus_tag="VKQ27_08495"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HLY89010.1"
                     /translation="MIPPLPLIMVEPAVRAALLEDLGRAGDLTTDAIVPASARTECAL
                     IARQPGVVAGLDYAATAFQLIDPSIEILVERADGSRLAPGDLIAIIRGPARGILTAER
                     TALNFLCHLSGVASATRGIVDAIVGTKAQVVCTRKTMPGLRAAQKYAIRVGGGSNHRF
                     GLDDAVLIKDNHVAIAGGVRAAVERARAGVGHLVKIELEVDTLAQLHEAMTLRVDAVL
                     LDNMGPDMLREAVGIVDGRAITEASGRIRPDTAPAIAASGVDLLSAGWLTHSAQVLDI
                     GLDHRG"
     gene            complement(6769..7752)
                     /gene="nadA"
                     /locus_tag="VKQ27_08500"
     CDS             complement(6769..7752)
                     /gene="nadA"
                     /locus_tag="VKQ27_08500"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLY89011.1"
                     /translation="MSHAEVLSRTAPLYQRVKSVIPPMEWPVFADDIDAILALKRQRN
                     AVILGHNYMTPEIFHCVSDIVGDSLALAREAMRVDADVIVLAGVHFMAETAKLLNPTK
                     TVLIPDTRAGCSLAESITAADVRLLRERYPGVPVVTYVNTSAAVKAESDICCTSGNAR
                     KVIESLGVDRVIMLPDEYLAQNIAAQTGVKIITWAGHCEVHERFTAEQIRSLRRDNPG
                     VVVLAHPECPPDVVAEADFTGSTADMSGYVDKQKPAKVVLITECSMADNVSVHHPDVE
                     FVRPCNLCPHMKRITLANIRTALEEMRHEVTIDPAIAVRARQAVERMLAVR"
     gene            7942..8340
                     /locus_tag="VKQ27_08505"
     CDS             7942..8340
                     /locus_tag="VKQ27_08505"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLY89012.1"
                     /translation="MASVRQEIHVKASPDDAWDAIRDIGALHVRLVPGFVVDTRLEPG
                     ARIVTFANGLVAKELIIDLDDAARRLVWSAQSERLSHHNASVQVFPAAEGSTVVWTCD
                     LLPDDMRGPIEGMITQGMAAMQRTLDAVGR"
     gene            complement(8436..9350)
                     /locus_tag="VKQ27_08510"
     CDS             complement(8436..9350)
                     /locus_tag="VKQ27_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89013.1"
                     /translation="MDQVRTRRRPAAEATAELIAVIVAVTGDAPRVLTIGGGAALPSG
                     PFELTHRSLQAGMRSWVERQTHHPLGYVEQLYTFADRDRTRTGQRIVAISYLGLTREV
                     RTSDEPGVTWQSWYRYFPWEDRRSGTPGVVETIVARLKAWAEATVDAMARRDRQRRIG
                     ATFGSAWNEELILQRYELLWEAGLVPESPEARDPIEGVAMTHDHRRILATGIARLRAK
                     IKYRPVVFELMPPRFTLLQLQQTVEALAGRLLHKQNFRRLIEQQDLVEETGEITTETR
                     GRPAKLFRFRRDVVAERAVAGSKLPLAR"
     gene            9542..>10442
                     /locus_tag="VKQ27_08515"
     CDS             9542..>10442
                     /locus_tag="VKQ27_08515"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLY89014.1"
                     /translation="MHEVQSDAIVFFGATGDLAYKQIFPSLLGLVRDEGLNVPIIGVA
                     KAGWGLDQLKERAADSLKHAGINDPTAQSRLMELLRYVDGDYNDPATFAELRNQLGEA
                     TRPLHYLAVPPSLFATVAEALANSGAAEDEARLVIEKPFGHNRETARTLSRLLGKYFA
                     SENILRIDHYLGKESVQNITYTRFANLMFEPLWNRDHIASIQISMPESFGVQDRGRFY
                     DETGAVRDVLQNHLLQVLAQLTMDPPTGEEHEATRDQKAALLKAVRPLDAAHVVRGQY
                     VGYQQVPGVRAGSQVETYVAVKLF"
BASE COUNT         1782 a         3257 c         3416 g         1987 t
ORIGIN      
        1 agcagaaaca gacgatctga tgcaaaatgc gcaggccaag cgggtgcgca agaaagcgga
       61 ctggattgtc gcgaacgatg tctcgcctga gaccgggatt atgggtggcg aggagaacgc
      121 ggtgcacctg attaccgcgg agggtgtgga ggactggccg cggctggcca agcaggaagt
      181 ggcgcggcgt cttgcggaac ggatcgcggt ggcgttgcgg tgagggggca ccgggagggc
      241 ggagccgatc agggaaggtc ggggcgttcg gcggggtcga gattgcttcc ccccggattt
      301 caccgggggc ctcgcaatga cattggggtg ctaagcggct ttcttctgtc tgggccagcg
      361 cgtggcgacc atcggcagta cttgctcgat cagcttgcgc gtctggtcca gcatgccctc
      421 atcgccccgt gccgccgggc gcaggatgaa ctttgacgcg cctgcttcga cgtaatcggc
      481 gatgcgatcg agcatcaggg ctgcgtcgcc gatcgcgaag tgccgttccg gatcgcggcc
      541 ggtgcgcttg cggtaggcct ccatcacctg tgcgacgccg ggatcgtcgg ctttgccgaa
      601 gtgataggcg aaggcggcgc cgtagtgatc ttcgtctata cagcgtccgg cttccaccac
      661 cgctgccttg atctccgcga tcacccgccc gacgtcggct ggggtttcgg cgccggcctg
      721 ccatcccgtg ccgtatcggg ccgtgcggcg gaccgctgcg gccgaggaac caccgatcca
      781 catcggcaga tcaggctgga ccggctttgg cgagatcgat gcgccggtca gatgattgtg
      841 ccggccctcg aaatcgacac tgtcctgcga ccacaggcgc gcgatgatct ccagcgcttc
      901 atcggtccgg cgtccgcgcg tcgcggtatc gatatgcaac gtggaccact cgggtccctg
      961 ggggctgccg atgccgaagc ctggcagcaa gcgaccctgt gacagtacgt cgatcgtcgc
     1021 gcactgcttc gccagcagca ctggatcgcg caacgcgagc gacaccacgt tgacgccgaa
     1081 cttgatgcgg cgcgtgcgac cggcgatcgc ggcgagcgcc gtcatgcatt ccaggatcgg
     1141 ctggcggctg acgatgcgat cggtctgcca gatggaatcg atgccacctg cctcgcacat
     1201 atccacccag cgccagaaac catcggtgct ggagaaaggg aactccatca ggccaacgcc
     1261 gatggcgatg ttgcgtgcca tggccatctc cgtcagttgt tgccgactgc ctcgcccaat
     1321 gtcgcgccga tctcccggtg cagcaccaga tcggcatagg gatccagccc cgtgggttcg
     1381 cggttcagga tcaccagtcg cgtgccgttg cgcgccgcca tctcggggaa tcccgccgcg
     1441 ggatacacca caagggacga tccaatggag atgaacagat cggctgccaa agtttcctct
     1501 tcggcgcgga tcatcgccgc gggaggcatc gattggccga acgataccgt cgctgtcttc
     1561 acgaacccgc cgcacgcatt gcagttcggc ggttcgcctg ttcgttcgaa ctcctcgcgc
     1621 acgtcgatga tctcgtagcg ctgtgcacag tcgaggcaat gtgcgtaggt cgtgttgccg
     1681 tgcagctcga tcacccggtt cgccggcacg ccggacgcct gatgcagccc atcaatattc
     1741 tgcgtgatca ccgccgaggc cttgccgcgg tcgatcagcg ccgcgactgc ccggtgcccg
     1801 cggttcggtt cggcgctgcg tatcgtctcg tcggtggcaa agcggcgacg ccacgtctcg
     1861 cggcgcgact ccgccgacga catgaactcg gaaaaatcga tcggcttcat cttcgtccag
     1921 atgccgccgg gactgcggaa gtcggggatg cccgattccg tgctgatgcc ggcgccggtg
     1981 aagaacacca cgcgctgggc ttcctcgatc atgcggcgca gttccgcgat gctcggatcc
     2041 atcgatccct ccagttaccg ctatgctcca ccagtgtggg cgtcgcggca ataggttgcg
     2101 gcaccgcgcg cgcgacggca ggatcgcggc aattacccga ggaacgttcc gatcgtgtct
     2161 gaaatcaccg tggccaggcc gatccgccga gcctggctga tcgacagtgc cgatttctgg
     2221 cgcttgtggt ctgtcggcct cgttgtgttc acggtgcggt gggtagagac ggtcgctgtc
     2281 ggcgtgttcg tttatgagcg caccggttcg gccttcctcg tggcgatgat gacgatgctg
     2341 cgactgctgc cgatggggct gttcggcgcg ttcctcggtg ccatggccga gcgcatcgag
     2401 cgccgcacct ctctgctgat tgtcgtggcc ggcatggtgg tgacatcggt gacgctggcc
     2461 accctcgcgt acctgaacca gctggaggtc tggcacctgg cagtcgccag cttcgtcaat
     2521 ggcatgggct gggctacgga caatcccgtg cgccgcgtca tgatcggcga ggcggtcggc
     2581 gcgtcgcaga tgggcacggc gatgtcgatc gacgtaggcg ccaacaacgc cagccgcatg
     2641 atcggtccga cgatcggcgg actgctgctg gcgacgatgg gcatcgccgg cgtcttcacg
     2701 ctcagcgtgg ttctgtacgt gatcgctctg tttgcggcgt ttcgcgtccg ctatcgcaac
     2761 agctttccat cggctgccgg cgagtctgtc ctggcgcgca tcgccgaggg actgcgcctg
     2821 gtgaggaacg acaagcgcct ggtcggcacg ctgaccgtga cggtgatcta taacgtgttt
     2881 ggctggccgt tcacgagcat ggttccggtg attggcaagg atcacctgca actcggtccg
     2941 gagggcatcg gagcactctc cagcatggac ggtatcggcg catttgtcgg tgctgttctg
     3001 atggcgatgc tgtgccggcc cgcgttctat cgttacgcgt atgtcggcgg cgtgttgctc
     3061 tacatggtga tgctgacggt atttgccctg gttccgatgc cggccctggc cgcatcggca
     3121 ctgctgttca cgggactggg cggcgccggc ttcagcatca tgcaggcgac gctggtgtat
     3181 ctttccgcgc cacccgaaat gcgcagccgc atgcttggcg tgctgtcggt gtgcatcggc
     3241 gtgggccccg taggctttgt ggcgcttggc tggctggcgg atacgatcgg cgcgcaatgg
     3301 gcaacggcgg cgaccggtat taccggcctg gtgatgatgc tgctggcgcg tccgttatgg
     3361 cgcggcatct gatcgatcga gatcgagcgc cataagctct tcatcgtaga acacatcgcc
     3421 gatgcggatt gcgcgccgtt cggtcccata cggcacaaac cccatgcgct ggtacaggcg
     3481 ttgtgccggg gcgttgccag ccgtgacgaa aagctgcagc accaatgcgc ctgccccgcg
     3541 cgcgtgatcg atcaccgctg agacaaggtc ccgggcgagg cctgatcgcc gatgcccggc
     3601 cgcgacgtac atgctgaaca gccccgcctt gtgccgctcc ttcgggcccg tgcgcaggtt
     3661 gaggccaacc agcccaacca gttcatcgcc agcgaatccg ccaaagcgtg tcaggccagg
     3721 tgcggccagc cgttctgcga actgtgcctc actgagctgc acttcatctt cgtacgatgc
     3781 ggtaaatgcc tcgggatgca gccgcaaagc gtccagccgg acgcggcgaa acgccgctac
     3841 atcatcctgt ccaagttcgc gcaccatgtg gctcatgcgc tatcaatgca cggtaggcgg
     3901 tgcttcgacc agatgcagac gcagtgcgcg aatgcgcgcg gtcctttgct cgctggtcag
     3961 tgacaggcgc cccggcagat cgcggaacgc aaccccggtc ggcgtaagga cgagcggcga
     4021 cggctcgcgc ttcggcggcg gtgctttctt ctgcgcctct ttcaggcgct tcttccgccg
     4081 tctttcgatg tcgcggcgca tgctctgcgc cagggacagg tttttacgcg ccaggctctc
     4141 ccaacctcct atcagcttga gctcaactcc gtgtttggcg gaattgcggg agcaatccca
     4201 ggccattgcc tcgcagacga caatgtcctt tgccgccatg gcgtcgaaaa tgtcgaaagg
     4261 gtcgagtgct atgaattcgt tgattgcggc ttgccggaac ctttcgcggt tttccggcgt
     4321 gtctggctca ggcggaggca ggaggctgca catgtcgtcg atcatgcggc gcagcacgtt
     4381 ttctggcagt tgggggaaac gcttggcgat atccatttgc agcctcgacg agccagcgat
     4441 gaacgggcat gctaatcgtt atctatatta actgcaactg ttaattgcgg ccagcgtatt
     4501 cgcgcttcag ctcgggaatg atctgtgtcg cgatggtttc gaaccttgcc gtgtcgtcgt
     4561 cgcgcagcgg gtagtacagt ccgatttcgg acacccccag cgccaggacc tgccgcacca
     4621 tgtcggcaaa agcatcgttc gatgcgtagt agttgactgg tccaccggtc cgacgggcct
     4681 gcacgtcgag catcagataa gatcgccgca gcgtggcggg atcgcgcccg atctttaggc
     4741 aatgctcgtc gaccagacgg atccgttcgc gtgtctcctt gatctgtgcg tcaaagctgt
     4801 cggtaaagct gatgctgttc caggtatccg catattgcgc cgcgtgtcgc agcatggtcg
     4861 gacccatcgc cgcgataacg attggcggcc gaggttgctg gatggggcga ggctgggtga
     4921 ccgcgctgtc ggcctggtaa tgttggccgt gaaacgtcgt cacgtcatcg cgcagcagcc
     4981 gatcgacgag ttggacatat tccgggaagc gcgccacgcg ctcgcgattg ctccaattcg
     5041 gcaggcccat catcgcgtag gaaggatcgg tcggcagacc cagtcccagt ccaagctcca
     5101 ggcgtccgtt ggagacatga tcaagtgtca gtgcctgatg cgcaagcaac gccggattcc
     5161 gcagcgggat ctgggctacg taggcgccca gcctgatccg gctcgtgcgc gaggcgacgg
     5221 cggtgagcag ggtgaagcac tcgaaccaga tgcctttgcc gccggtccaa tcggcgaaat
     5281 gatcggcgaa ggccgcaatg tcgaagccga gttcctccag gtgccgatac cggcggagca
     5341 attccggcca gggctggttc ggcaggatca tggtgaagaa gcgcaagtcg tggggcatgg
     5401 ccgcctccgg gagacatgag ccgcgtttgt tcgaaggcta tcggatatcg gcggcggggt
     5461 cttgccgata cggtattatg ctctacgcaa tgcatatagg atggcataat cgccgcgatg
     5521 atccagagct tcaggtgcgg caggaccgaa gcgctgttca ggggcgaaag cgtcgatcgg
     5581 cgctggcgcg gtttcgaaaa ggtcgcgcgc cgcaagttgt tgatggtgga tgcagagcat
     5641 tccctctgcg atctgaccgt gccgccggcc aaccggtcag aggttctgaa gggcgatagg
     5701 cgtaccgacc aaccgtatca ccgagatcgt caacggtacc cgaggcgtta ccggcgagac
     5761 gaccgtcctg ctaagccacg cctttcgcac tacgccggag ttctggttga atttgcagtc
     5821 ccgctacgaa cttgacctgg ccaatacccg agtgccgagg gaggcaatcc agcgtgcgga
     5881 tgcgttcaac aaggagctga gggcggctta gcgcgaagcc aagctagccc cgatgatcca
     5941 gcccgatatc cagcacctga gcactgtgcg tgagccaacc tgccgacagc aggtccaccc
     6001 cgcttgccgc gatcgcgggt gcggtatccg ggcggatgcg cccggaggct tcggtgatgg
     6061 ctcgaccatc gacgatgcca actgcctcgc gcagcatgtc ggggcccata ttgtcgagca
     6121 ggactgcgtc cacccgtagc gtcatcgctt cgtggagctg tgccagggta tccacctcca
     6181 gctcgatctt caccagatga ccgacgccgg cacgcgcacg ttccaccgcc gcacgcactc
     6241 cgccggcgat cgcgacgtga ttgtccttga tcagcacggc gtcatcgagt ccgaaacggt
     6301 ggttcgaccc gccgccgacg cgtattgcgt atttctgcgc ggcgcgcagc ccgggcatgg
     6361 tcttgcgcgt gcacaccacc tgcgccttcg tcccgacgat cgcatccacg atgccgcgcg
     6421 ttgcggatgc cacgcccgac aggtggcaca ggaaattcaa cgcagtgcgc tctgccgtca
     6481 ggataccgcg cgccggtcct cggatgatcg cgatgaggtc acccggagcc aggcggctgc
     6541 catcggcgcg ttccaccaat atctcgatcg atggatcgat cagctggaac gccgtcgcgg
     6601 cgtaatccag gcccgccacc acgccgggtt ggcgggcaat cagggcgcat tccgtgcgcg
     6661 cgcttgccgg cacgatcgcg tccgtcgtga ggtcaccggc gcgcccgagg tcctccagca
     6721 gcgccgcgcg caccgctggt tcgaccatga tcagggggag gggtgggatc atcgtactgc
     6781 cagcatccgc tcaactgcct ggcgtgcacg tacggcaatc gcaggatcga tcgtcacctc
     6841 gtggcgcatc tcctccagcg ccgtgcggat attcgccagc gtgatgcgct tcatgtgcgg
     6901 gcataggttg cacggccgca cgaactccac gtccggatgg tgcacggaca cgttgtccgc
     6961 catcgagcac tcggtgatca gcaccacttt cgccggcttc tgcttgtcca cgtagccgga
     7021 catatcggcg gtggagccgg tgaaatcggc ctcggccacc acgtcgggcg ggcattcggg
     7081 atgcgccagc accacgacgc ccggattgtc gcgccgcagc gaacgaatct gctcggcggt
     7141 gaagcgttca tgcacctcgc aatggccggc ccaggtgatg atcttcaccc cggtctgcgc
     7201 ggcgatgttc tgcgccaggt actcgtcggg cagcatgatc acgcggtcta cgccgagcga
     7261 ttcgatcacc ttgcgcgcgt tgcccgaggt gcagcagatg tcggactccg cctttaccgc
     7321 ggccgacgtg ttgacatatg tcaccacggg cacgccggga tagcgctcgc gcagcagccg
     7381 gacgtcggct gccgtgatgg attccgccaa agaacagccg gcacgcgtgt ccgggatcag
     7441 cacggtcttg gtcgggttca gcagcttggc ggtctctgcc atgaagtgca cgccggccag
     7501 cacgatcacg tcggcgtcca ccctcatggc ctcgcgggcc agcgccaagc tatcgccgac
     7561 gatatcggag acgcagtgga agatttccgg cgtcatgtag ttgtggccca ggatcaccgc
     7621 gttgcgctgg cgcttgagcg ccaggatcgc gtcgatgtcg tcggcgaaca ccggccactc
     7681 catcggcggg atgacggatt tcacccgttg atagagcggt gcggtgcggc tcaggacctc
     7741 ggcatgggac atctggacag cctccttatg ctccaactga gcatttcccc tcttatgcta
     7801 gtgatgagca taaggatgtc aagagggagg taactttggg ggcggttgcg gcgaccttgg
     7861 ttaaagaggt ccgatttcgg ccagacgacg ctggtgtcgg cagttatcgt cggcgtgact
     7921 caagatctgg gaaggcatga gatggcatcg gtccgccagg agatccatgt gaaggcaagt
     7981 ccggacgatg cgtgggacgc gatccgcgac attggcgcct tgcatgtccg tctggtgccc
     8041 ggcttcgtcg tcgatacgag acttgagcct ggggctcgca tcgtcacatt cgccaatggc
     8101 ctggtcgcca aggaactgat catcgatctg gacgatgccg cgcgccgtct tgtctggtcg
     8161 gcccagagtg aacgacttag ccaccacaat gcatcggtac aggtttttcc ggccgccgag
     8221 ggatccacag tcgtctggac ttgcgacctg ttgccagacg atatgcgcgg cccgatcgag
     8281 ggcatgatca cccaggggat ggcggcgatg cagcgcaccc tcgatgcggt tggccgataa
     8341 cggccggctg tcgctcatcc cccatcctag gccgcaacgt acctgcgagg agtgatggcc
     8401 ggttcagggg tgggtcgcgc agtcgatctg ccgacctatc gtgccagcgg gagcttgctg
     8461 ccggcgacgg cgcgctcggc gaccacatcg cgccggaagc ggaacagctt tgctggccgg
     8521 ccacgcgttt cggtggtgat ttcgccggtt tcttcgacga ggtcctgctg ttcgatcagt
     8581 cgccggaaat tctgcttgtg gagcagccgt cctgcgagtg cttcgaccgt ctgttgcaat
     8641 tgaagcaagg tgaaccgagg cggcatcagt tcaaacacca ccggccggta tttgatcttc
     8701 gcccgcagac gagcgatgcc ggtggccagg atacggcgat ggtcgtgtgt catcgccaca
     8761 ccctcgattg gatcgcgcgc ctcgggggat tccgggacca gcccggcctc ccacagcagt
     8821 tcgtagcgtt gcaggatcag ttcctcgttc cacgccgaac cgaacgtggc gccgatccgc
     8881 cgctgacggt cccggcgggc catcgcatcg accgtggcct cggcccacgc cttcagccgc
     8941 gccacaattg tctccacgac ccctggagta ccgctgcgcc ggtcctccca cgggaaatac
     9001 cggtaccagc tctgccaggt cacgcccggt tcgtcagatg tccggacttc tcgggtcagc
     9061 ccaagatagc tgatcgccac gatgcgttgc ccggtacgcg tccggtcccg atcggcgaag
     9121 gtgtaaagct gctcgacata gccgagcggg tggtgggtct gccgctccac ccaggagcgc
     9181 atgcccgcct ggagggaccg gtgcgtcagc tcgaacggac cggatgggag ggcggcgcct
     9241 cctccgatgg tcaggacgcg cggagcatcg ccggtgaccg cgacgatcac tgcgatcagc
     9301 tcggcggtcg cctccgccgc gggtcgccga cgcgtgcgca cctggtccat gcgagtcgct
     9361 ttatgcagga agccgtcccc tattcgcacg ccaggcgaca ggccgccaac acagataagt
     9421 gagacactcg cccctccgcg gccggtatct cgcggccccc tatgtcgcaa atccgaacaa
     9481 gacggcgcgc gatgttcccc ctaaggtggt tgtgcgcggg catgaaccag aggtatgcga
     9541 gatgcacgag gtccagtcgg atgcgatcgt ttttttcggc gccactggcg acctggcgta
     9601 caagcagatt ttcccatcgc tccttggtct ggtccgtgac gagggcttga acgttccgat
     9661 cattggcgtc gccaaggccg gctggggcct ggaccagttg aaggagcgtg ccgccgacag
     9721 cctgaagcac gccggcatca atgacccgac ggcgcaatcc aggctgatgg agctgcttcg
     9781 ctacgtggat ggcgactata atgacccggc cacgttcgcg gagctgcgga accagctcgg
     9841 cgaagcgacg cggccgctgc actaccttgc cgtgccgccc agcttattcg ccacggttgc
     9901 ggaggcgctg gccaactcgg gtgcggcgga agatgaagcg cgcctggtga tcgagaagcc
     9961 gttcggccac aaccgcgaga cggcacgaac gctgagccgg ctgctcggca agtatttcgc
    10021 cagtgagaac atcctgcgca tcgaccacta cctgggcaag gagtcggtgc agaacatcac
    10081 ctatacgcgg ttcgccaacc tgatgttcga accgctgtgg aaccgcgacc acatcgccag
    10141 catccagatc tcgatgccgg agagttttgg tgtgcaggat cgcggccgtt tctatgacga
    10201 gacaggtgcg gtgcgcgacg tattgcagaa ccatctgctg caggtactgg cacagctcac
    10261 gatggacccg ccgacgggcg aggagcacga ggcgacgcgc gaccagaagg ccgcgctgct
    10321 gaaggcggtg cgtccgctgg atgcggcgca cgtggtgcgc gggcagtatg tcggttacca
    10381 gcaggtgccg ggtgtgcggg cagggtcgca ggtcgagaca tatgttgcgg tcaagctgtt
    10441 ca
//
LOCUS       DATGEM010000272         2458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_219869, whole genome shotgun sequence.
ACCESSION   DATGEM010000272 DATGEM010000000
VERSION     DATGEM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219869"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..330)
                     /locus_tag="VKQ27_08520"
                     /pseudo
     CDS             complement(139..330)
                     /locus_tag="VKQ27_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            complement(330..>2458)
                     /locus_tag="VKQ27_08525"
     CDS             complement(330..>2458)
                     /locus_tag="VKQ27_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HLY89015.1"
                     /translation="PEQIRIYDAYAGAFSIIHNNLDAAMRAANITGETGTLNAQAKSA
                     ARSAFESAKQRFFGHLLTSMKTPSLTRSIERDLDAGYAAVIQIVSTGEALMERRLAEI
                     PTEEWGDVQVDITPREYVLDYLAHSFPVQLYEPFTDSEGNLCSRPVYHDGQPVESREA
                     VTRRDRLIEKLASLPPVPGALDQIVQRFGTDMVAEVTGRSRRVVRKAGRLIVENRAAS
                     ANLAETAAFMDDAKRILVFSDAGGTGRSYHAELSARNRRLRVHYLLEPGWKADAAIQG
                     LGRTNRTNQAQPPLFRPIATDVKAEKRFLSTIARRLDTLGAITRGQRQTGGQGMFRPE
                     DNLESFYGRDALRQLYMLLVHGKVEGCSLERFEDATGLKLMDANGIRDELPPITTFLN
                     RLLALTIDLQNVLFTAFEQLLTARIEGAIASGTYDVGLETLRAESFVITNRRVIYTHP
                     STGAETRLLTITERRRNHPVTLEEALDRLSEVVLLINERSGRAAIQVPAPSLMLDDGE
                     IERRVRLIRPMEHNSLPLKMMPESHWAEADCERFAAAWQAELAQVPEFTDSTIHVVAG
                     LLLPIWKRLPNESTRVYRLQTDTGERIIGRKVSPAWVATTLAGDATALTPDAAFAALL
                     EGRTVLELAEGLKLRRARVMGTHRIELSDFTDGMRDRLRAYGLFGEIISWKLRMFVPT
                     DANGVGVLAKVFECYPVERVGEREAA"
BASE COUNT          439 a          764 c          820 g          435 t
ORIGIN      
        1 ggcgcgccag ttcagaagcg tcgcgggaca tcacgagacc tcccgcagcg cactgacatt
       61 atgttgcgta gaagccgtcg atcgtctggg ggaaagcgcc caatcgcggg ggcgcaacat
      121 aactctcggc gcaacatgtt ctgtccactt gccggcaggt cccttcgggg agtcctggag
      181 ccgtacaaac attgaacggc ccggactatt gtggacatcg ccaaccagcc aataccgccc
      241 ttcgcgcctg ccgttgaaga gatagtggcg gcacacggct tcagcctcac gcgcgagacg
      301 gcgtgccaga tcggaagcgt cgcggtacat cacgcggcct cccgttcacc cactcgttcg
      361 accgggtaac attcgaacac cttggccagc acgccgacgc cattggcgtc agtcggaacg
      421 aacatgcgca gcttccagga aatgatctcc ccgaagagcc cataagcccg caggcggtcc
      481 cgcatgccgt cagtgaaatc agacagttcg atgcgatgtg tgcccatcac gcgggcgcgc
      541 cgaagcttca gcccttcggc cagctcaaga accgtgcgac cttcgagcag cgcggcgaag
      601 gcggcgtccg gcgtcagcgc cgttgcgtcg ccagccagcg tagtcgccac ccaggcgggc
      661 gagaccttcc gaccgatgat gcgctcgccg gtatccgtct gaagccggta gacgcgggtg
      721 gactcgttcg gcagccgctt ccagatcggc agaagcaagc ctgccacgac atggatggtg
      781 ctgtcggtga actcgggcac ttgggcgagt tccgcctgcc aggccgcggc gaagcgttcg
      841 cagtcggctt ccgcccaatg gctttccggc atcatcttca gcggaaggct gttatgctcc
      901 atcggtcgga tcagccgcac gcgccgctcg atctcgccgt cgtccagcat gagactcggc
      961 gcaggaactt gaatggcggc gcggccagaa cgctcgttga tcagcaggac gacctcggag
     1021 agccgatcga gcgcctcctc cagcgtcacg ggatgattgc gccgacgttc ggtgatggtg
     1081 agcaaccggg tttcggcacc ggtgctggga tgcgtgtaga tgacacgccg gttggtgatg
     1141 acaaagctct cggcacgcaa ggtctccagg ccgacatcgt aggtgccgga cgcgatggcg
     1201 ccctcgatgc gcgcggtgag caactgctcg aacgccgtga acagcacgtt ctgcaggtcg
     1261 atggtcagcg ccagcagcct gttcaagaat gtggtgatcg ggggaagctc atccctgatg
     1321 ccgttggcat ccatgagctt gaggccggtc gcgtcctcga acctctcgag cgagcatccc
     1381 tcgaccttcc cgtgcaccag cagcatgtaa agttgacgca gtgcgtcgcg cccgtagaaa
     1441 ctttcgagat tgtcctcggg ccggaacatg ccctgcccgc cggtctggcg ctgaccgcgc
     1501 gtgatcgcgc cgagcgtgtc gagacggcgc gcgatggtgc tcaagaagcg cttctcggcc
     1561 ttaacgtcgg tggcgatcgg tcggaacagc ggcggctgcg cctggttggt acggttcgtg
     1621 cggccgaggc cctggatggc tgcatccgcc ttccagccgg gctcgagcag gtaatgaacc
     1681 cgcaacctgc gatttcgcgc cgacagttcg gcatggtacg aacggccagt gccgccggca
     1741 tcactgaaga caaggatgcg cttggcgtca tccatgaagg cggcggtctc tgcgaggttt
     1801 gccgatgcgg cgcggttctc aacgatgagg cggcctgcct tacggacgac gcggcgagag
     1861 cgtcccgtaa cctcggcgac catgtccgtg ccgaaccgct ggacaatctg gtcgagcgcg
     1921 cccggcacgg gcggaagcga ggcgagcttc tcgatcagac ggtcacggcg ggtgacggct
     1981 tctcggctct caaccggctg gccgtcgtga tagaccgggc gggagcagag attaccctcg
     2041 ctgtcggtga agggctcgta gagctggacc gggaaggaat gggcgagata atcgagtacg
     2101 tactcgcgcg gagtgatgtc gacctggacg tcgccccact cctcggtcgg gatctccgcg
     2161 agccggcgct ccatcagcgc ctcgccggtc gagacgatct ggatgacggc ggcatagccg
     2221 gcgtcgagat cgcgctcgat cgagcgggtc agcgagggtg tcttcattga ggtgagcaga
     2281 tggccgaaga agcgctgctt ggcgctttcg aaggcggatc gcgccgcgga ctttgcctgc
     2341 gcgttcagcg tgccggtctc gccggtgatg ttggcggcgc gcatcgcagc gtcgagattg
     2401 ttatggatga tgctgaatgc gcccgcataa gcatcgtaga tacggatctg ctcgggcg
//
LOCUS       DATGEM010000273         4680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1100109, whole genome shotgun sequence.
ACCESSION   DATGEM010000273 DATGEM010000000
VERSION     DATGEM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100109"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..976)
                     /locus_tag="VKQ27_08530"
     CDS             complement(194..976)
                     /locus_tag="VKQ27_08530"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HLY89016.1"
                     /translation="MLKGKSAIVTGSTSGIGLGIARALAAQGCNVMLNGFGAGPQIEK
                     LRAALAAEHQVTVLYSSADMSKPLEVAAMVDQVARRFGSVDVLVNNAGIQHVAPVEEF
                     PVEKWDQILAINLSSAFHAIRIALPGMRARNWGRIINIASAHGLVASPFKSAYVAAKH
                     GLVGLTRTVALETAGKGITCNAVCPGFVLTPLVETQIQDRAREHGISREDAITNVILE
                     RQPSREFVKIEEVAALTVFLAGDAAASITGAAYSIDGGWTAQ"
     gene            complement(1020..1799)
                     /locus_tag="VKQ27_08535"
     CDS             complement(1020..1799)
                     /locus_tag="VKQ27_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89017.1"
                     /translation="MLVAAGHRLEYVMLQPQNAGAGDRPTLVFLHEGLGSVAMWKRFP
                     AEIVAATGCAALVYSRYGYGRSDKLTQSRKVDYMHREALDVLPEVLDRLDIHAPVLIG
                     HSDGASIALIHAGAGKRPVRGVVAMAPHVFVEDITVRSIAAAMTTFRTTDLPQRLARY
                     HDDAESAFQGWNDIWLHPDFLRWNIETFLPGVACPLLLIQGEDDQYGTAAQVEAIARQ
                     VSGPVDTLMLPDCAHSPHQSMQKDATVAAIARFVAHLGKTG"
     gene            complement(1800..2513)
                     /locus_tag="VKQ27_08540"
     CDS             complement(1800..2513)
                     /locus_tag="VKQ27_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89018.1"
                     /translation="MPEVLIEARGIVAGYGVSRVLHGVDLVLKRGEAVGLMGRNGMGK
                     TTLLRSILGLLPLQAGEVKVRGVDMTGAPAHRIARAGIAYVPEGRGIFADLSVEENLL
                     MAARPGCDGRRDWTLARVLSAFPRLAQRLRHGGQQLSGGEQQMLTVGRALMTNPDVLI
                     LDEATEGLAPLVARDIWAILRQLRDTGLSTIIVDKNFAVVSALADHNIVLVKGRIVLD
                     GSALDLKRDPQLLQRHLGI"
     gene            complement(2524..3276)
                     /locus_tag="VKQ27_08545"
     CDS             complement(2524..3276)
                     /locus_tag="VKQ27_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89019.1"
                     /translation="MAESILQARDLSRHFGGLAALSEVSLACEMGQVHAVIGPNGAGK
                     STLVNLLSGELAASAGQVLFEGRDITGLAADRISQLGVARSYQKTNLFRQFTAFENCR
                     LAAQSRLRTSMHFFRPALTREDINAEAHRALAQAGLADRHDVVVSALSHGEQRQVEIA
                     MALATRPRMLLLDEPLAGMGAEESARIVALLHELARDHAILLVEHDMDAVFAVANTLT
                     VMVNGRVLESGSPARIRASRAVQEAYLGADSD"
     gene            complement(3269..4246)
                     /locus_tag="VKQ27_08550"
     CDS             complement(3269..4246)
                     /locus_tag="VKQ27_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89020.1"
                     /translation="MMPRSRSLAIVAILIVAALVLLPFVAEKFTVQFATRILIMAMFA
                     MSLNLLVGHAGLVSLGHAAYFGIAGYCLALASQQSQAANFWTSLPLVMIAAGALALVI
                     GALVLRTGGIYFIMATLAFAQMLYFLLHDTGIAGGSDGMYIYARPDASLFGWRPIDLE
                     RFGDFYFVVLALFVVVFGFIGALRASLFGRVLAGIRVNEQRMRSLGFATFRYKLACFV
                     LAGMLAGLAGYLAAVQFGVVNPDMLGWQLSGAVLMMVILGGMGTLAGPVIGAAAMLLL
                     ELGLQSLPALGDFNPGKHWQLSMGIVIVLVALFLPEGITRLLRRTAADG"
     gene            complement(4243..>4680)
                     /locus_tag="VKQ27_08555"
     CDS             complement(4243..>4680)
                     /locus_tag="VKQ27_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89021.1"
                     /translation="LAVLLWFLIQRTRLGMMIRAGAANREMVQSLGIDIDLLYRFVFA
                     LGVALAALAGMIAAPVSSVFPGMGNQVLIVCFVVVVIGGIGSVKGALVGALLIGLVDT
                     FGKVVELKVGSFRLLPELAGMSVYLLMAVVLLWRPAGLFGKRA"
BASE COUNT          909 a         1539 c         1471 g          761 t
ORIGIN      
        1 cgaactttct cacaatttcc atggcttctc ctctgttgtt gggaaacgaa cagacaccac
       61 ttcaactgct gctgcaatca ccgcctggcg ctgtggacag gcgggcccga caaggtccgg
      121 gccgatgttc tggatgcccg actcgcggaa ctcatttctt tgtccggtcc tcctatcttt
      181 tctctgtacg gcgctactgc gccgtccacc cgccatcgat cgaatacgcg gccccggtga
      241 tcgaggccgc tgcgtcgccg gcaaggaaca cggtcagtgc ggcgacttcc tcgattttca
      301 cgaactccct ggacggctgg cgttcgagga tgacattggt gatcgcgtct tcgcgcgaaa
      361 tcccgtgctc cctggcccga tcctggatct gggtttccac cagcggcgtc agtacgaagc
      421 ccggacacac ggcattgcag gtgatgccct tgccggcggt ctccagcgcg accgtcctgg
      481 tcaggccgac caggccgtgc ttggcggcga cataggccga cttgaacggc gacgccacca
      541 gaccgtgcgc ggaggcgata ttgatgatgc gcccccagtt gcgcgctctc attcccggca
      601 gcgcgatgcg gatggcgtga aacgcggagg aaaggttgat cgcaagaatc tggtcccatt
      661 tctcaaccgg gaattcctcc acgggtgcaa cgtgctggat gccggcgttg ttgaccagca
      721 cgtccaccga accgaatcgc cttgccacct gatccaccat tgcggccacc tccagcggtt
      781 tggacatgtc cgcggacgaa tacagcacgg tgacctggtg ttcggcggcc agcgcggcgc
      841 gcagcttttc gatctgcggt ccggctccga atccgttcag catgacgttg caaccctgcg
      901 cggccaatgc gcgcgcgatg ccgagcccta tgccgctggt ggacccggtg acgatggcgg
      961 atttgccttt caacatgttc gctgctcttc caaaagtaag attcagtgta acagacggtt
     1021 cagcccgttt tgcccagatg agctacgaag cgggcgatcg ccgcgaccgt ggcgtccttc
     1081 tgcatgctct ggtgcggcga atgcgcgcaa tctggcagca tcagggtgtc gaccggaccc
     1141 gatacctggc gcgcaatcgc ctcgacttgc gccgcggtgc catattggtc gtcttcgccc
     1201 tggatcagca gcagcgggca tgcgacgccg ggcaggaacg tttcgatgtt ccagcgcagg
     1261 aagtcgggat gcaaccagat gtcgttccag ccctggaacg ccgactcggc atcgtcgtgg
     1321 tagcgggcga gccgctgcgg caggtcggtg gtccggaacg tggtcatcgc cgcggcgatg
     1381 ctgcgcacgg tgatgtcctc gacgaagaca tgcggcgcca tggcaacgac gccacgcacc
     1441 ggacgcttgc cggcgcccgc gtggatcagc gcgatcgacg cgccgtcgct gtggccgatc
     1501 aggaccggcg catggatgtc gagccggtcg aggacttcgg gcagcacgtc gagcgcctcg
     1561 cgatgcatgt agtcgacctt gcgcgattgc gtgagcttgt ccgacctgcc gtaaccgtaa
     1621 cgcgaataca ccagcgcggc gcagccggtg gccgcaacga tttcggccgg aaagcgtttc
     1681 cacattgcca ccgagccgag gccttcgtgc aagaacacca gcgtcggccg atcgccggcc
     1741 ccagcattct gcggctgaag catgacgtat tcgagacggt gcccggctgc gacgagcatt
     1801 cagatcccca ggtgacgttg caggagttga gggtcgcgct tcaagtccag ggcgctgcca
     1861 tcgagaacga tccggccctt gaccagaacg atgttgtggt cggcgagcgc ggacaccacc
     1921 gcgaaattct tgtccacgat gatggtggac aggccggtat cgcgtaactg tctgaggatg
     1981 gcccagatgt cgcgtgcgac cagcggcgct aagccttctg tggcctcgtc gaggatcaat
     2041 acatccggat tggtcatcag ggcgcggcca acggtcagca tctgctgttc gccaccggat
     2101 aattgttgcc cgccgtgccg cagacgttgc gcaaggcgcg gaaatgccga cagcacgcgc
     2161 gccagcgtcc agtcgcgcct gccgtcgcat ccgggccgcg ccgccatgag cagattttcc
     2221 tcgacgctca gatccgcaaa gatgccgcga ccttccggca cgtaggcgat gcccgcccgc
     2281 gcgatgcggt gtgccggcgc gccggtcatg tccacgccgc gcactttcac ttcccctgcc
     2341 tgcaagggca gcaatccgag gatgctccgc agcagcgtgg tcttgcccat gccattgcgc
     2401 cccatcagcc cgaccgcttc gccgcgcttc aataccagat ccacgccgtg caacacgcgg
     2461 ctcaccccgt agcccgccac gatgcctctg gcttcgataa gtacctcagg cacgggtgca
     2521 gccttagtca ctatccgcac caagataagc ctcctgcacg gcgcggctgg cgcggatccg
     2581 ggcaggcgaa ccggattcga gcacgcggcc gttgaccatg acggtcagcg tgttggcgac
     2641 cgcgaatacg gcatccatgt cgtgttcgac cagcaggatg gcatggtcgc gcgccagttc
     2701 gtgcagcaat gcgacgatgc gtgcggactc ctcggcaccc atgccggcga gcggctcgtc
     2761 gagcaaaagc atgcgcgggc gcgtggccag ggccatggcg atctcgacct ggcgctgttc
     2821 gccgtgcgac agcgccgaca cgaccacatc atggcgatcg gcaagaccgg cctgcgcaag
     2881 cgcgcgatgc gcttcagcgt tgatgtcctc ccgcgtgagc gccggccgga agaaatgcat
     2941 cgacgtgcgc aggcgcgact gcgccgccag ccggcagttc tcgaaggcgg tgaactgccg
     3001 gaacagattg gttttctgat agctgcgcgc tacgcccagt tgggagatgc gatccgcggc
     3061 caacccggtg atatcgcggc cctcgaacaa cacctggccg gcgctcgcgg ccagctcgcc
     3121 ggacagcaga ttgacgagcg tgctcttgcc ggcgccgttc ggaccgatga cggcatgcac
     3181 ctgtcccatt tcgcaggcga gcgagacctc gctgagtgcg gccagcccgc cgaagtggcg
     3241 cgataaatcg cgcgcctgca ggatcgattc agccatccgc cgcggtccgt cgcaacaggc
     3301 gggtgatgcc ctcgggcagg aacaacgcca ccagcacgat gacgatgccc atcgacaact
     3361 gccagtgctt gccggggttg aagtcgccca gcgccgggag cgactgcaaa cccagttcca
     3421 gcagcagcat ggccgcggca ccgatgaccg gaccggccag cgtacccatg ccgccgagaa
     3481 tcaccatcat cagcacggca ccggacaatt gccagccgag catgtcggga ttgaccacgc
     3541 cgaattgaac cgccgcgaga taacccgcca ggccggccag cataccggca aggacgaagc
     3601 aggccagttt gtagcggaac gtcgcgaaac cgagcgagcg catgcgctgc tcgttgacgc
     3661 ggatgccggc gaggacgcgg ccgaacaacg atgcacgcaa tgcgccgatg aagccgaaaa
     3721 caacgacgaa cagcgcgagc acgacgaaat agaaatcgcc gaatctctcc agatcgatcg
     3781 gtcgccagcc gaacaggctg gcatcgggcc tggcatagat atacatgcca tcggacccgc
     3841 cggcgatgcc ggtgtcgtgc agcaggaaat agagcatctg cgcgaacgcc agcgtggcca
     3901 tgatgaaata gatgcctccg gtgcgcagta ccagggcgcc gatgaccagc gccaacgcgc
     3961 cggctgcgat catcaccagc ggcaggctgg tccagaaatt cgccgcttgt gattgctgcg
     4021 aagccaacgc caggcagtag cccgcgatac cgaagtaggc cgcgtggccg aggctgacca
     4081 gcccggcgtg gccgaccagc aggttcaggc tcatggcgaa catggccatg atcagaatgc
     4141 gggtggcaaa ctgaacggtg aatttctccg ccacgaacgg cagcaggacg agtgcggcga
     4201 cgatgaggat ggcaacgatg gccagggaac gcgagcgcgg catcatgccc gtttcccgaa
     4261 caagcccgcc ggccgccaga gcaggacaac ggccatcagc aggtagacgc tcatgccggc
     4321 cagttccggc agcaaccgga aactgccgac cttcagctcg acgaccttgc cgaaggtatc
     4381 caccaggccg atcagcaacg cgccgaccag cgcgcccttg actgaaccga tgccgccgat
     4441 gaccaccacc acgaagcaga cgatgagcac ctggttgccc atgcccggaa ataccgaaga
     4501 caccggcgcg gcgatcatgc ccgccaatgc agccagtgcc acgccgagcg cgaacacgaa
     4561 gcgatacaac agatcgatgt caatgccgag cgactgcacc atctcgcggt tggcggcgcc
     4621 ggcgcggatc atcatgccca gacgggtgcg ctggatcaga aaccagagca gcactgcgag
//
LOCUS       DATGEM010000274         6614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_586754, whole genome shotgun sequence.
ACCESSION   DATGEM010000274 DATGEM010000000
VERSION     DATGEM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586754"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VKQ27_08560"
     CDS             complement(<1..518)
                     /locus_tag="VKQ27_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLY89022.1"
                     /translation="MTDAAAEIARRRTFAIISHPDAGKTTLTEHLLRAGGAIQLAGNV
                     RAKGERRRTRSDWMGIERDRGISVVTSVMTFEHAGCVFNLLDTPGHEDFSEDTYRTLT
                     AVDSAVMVIDAAKGIEARTRKLFEICRLRDIPIVTFINKMDRESRDPIELLDEVASTL
                     ALDTAPVTWPVGR"
     gene            complement(687..1349)
                     /locus_tag="VKQ27_08565"
     CDS             complement(687..1349)
                     /locus_tag="VKQ27_08565"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLY89023.1"
                     /translation="MFAVIFEVVPKTERKDEYLELAKFLRPEIEKIDGFLDNERFASR
                     SVPGRVLSLSTWRDEKSVIRWRTLAVHHQVQEKGRFEVFQDYHLRVGEFTADSRPPAG
                     QVLRQQRLDETEVGAARYVTISELGPRDGKPATDDLVRDLDLPASGTQGLLDRDTYES
                     IYVAGKLLLLASWRDVGAADAWRPRAVGQTELWHRTVRVVRDYGMFDRREAPQYYPEV
                     KR"
     gene            complement(1361..2326)
                     /locus_tag="VKQ27_08570"
     CDS             complement(1361..2326)
                     /locus_tag="VKQ27_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY89024.1"
                     /translation="MQATKLDEREQHFWIPSTVSGLNLFLRNLSPKQPAARGKVVLYV
                     HGGTFPSALSIAHRFDGRSWRDELCERGFDVWGLDFVGFGASDRYPEMAEPAECHGVL
                     GSAEMASRQIECAARWICAHHTVPRISIIAHSWGTIATGRFAGDCPDLVDRLVFFGPL
                     TWRAPTAEAQRYPAWRLVSVEDQWTRFTAEVPAGEPSVLSRRHFDEWGPLYLDTDPES
                     RVRTPPSVRIPSGPWQDIADAWAGRLAYDPGLISAPVSIIRGEWDSLATDADARWLFD
                     ALRASPLRRDVKISRATHLMHLEEGRYALYRETEAFLRGDDQPMA"
     gene            2488..3735
                     /locus_tag="VKQ27_08575"
     CDS             2488..3735
                     /locus_tag="VKQ27_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLY89025.1"
                     /translation="MDTAIPPSTLAARSWPDGGTWRVPNWVYTDPVVFQREMERIFSA
                     SDWLYVCLEAELPKPGDFRRSQLGNREVIAVRGADGAINVLVNRCSHRSMQVCMASRG
                     SAKEFVCPYHQWTYDLAGNLLGVPFRRGYRGQGGMPDDFQPEQHGLQRLAVTTRHGVV
                     FASFGAPDESLETYLGDRMLGYFDRVFDGRDLIVLGYMRQRIPSNWKLMFENIKDPYH
                     ASLLHVFLVTFGLFRLDQQSAVEMDDTGRHAVLVSRRGAQESNEATSQMRAFRQNFAL
                     RDKRLLDPVREFPGDATVVLQTLWPNLIVQQQSNTLAMRQIVPLDARSFDLAWTFFGY
                     ADDTPEMRQRRLRQANLMGPAGLVSLDDSEAMLLSQAGIDQDDGAACLVEMGGRAVGN
                     EPHMVTETAIRGFYQHYRAVMGL"
     gene            3732..4229
                     /locus_tag="VKQ27_08580"
     CDS             3732..4229
                     /locus_tag="VKQ27_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY89026.1"
                     /translation="MIAVAYGLTAGCELRARLADLYCAYDNALNDGELEQWPALFAED
                     CIYKVIPRENVEQGLPVAVIYCESRDMLTDRVVALRETALFAPRIARRMTTAICLRTI
                     DGDGMHLTASFAVFQTMPDRPSELFLCGSCRDRVMDDAGTLRFAERVFIYDSTIVPTS
                     LVHPL"
     gene            complement(4265..4588)
                     /locus_tag="VKQ27_08585"
     CDS             complement(4265..4588)
                     /locus_tag="VKQ27_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLY89027.1"
                     /translation="MAFYELRQYHVRPGRQAEWVKVMEEEIIPFQVSKGMVICGSFRG
                     ETDESVYIWLRRFNSEAEREAQYKAVYDTDYWKTKIAPRVPEMLDREKMIITRMVPTA
                     LSTMQ"
     gene            4767..5744
                     /gene="hemB"
                     /locus_tag="VKQ27_08590"
     CDS             4767..5744
                     /gene="hemB"
                     /locus_tag="VKQ27_08590"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HLY89028.1"
                     /translation="MASPAFPTTRLRRLRRSEPLRALVRETTVTANDLIQPIFVEEGI
                     DEALPIAEMPGVSRIPERKIEHVVEGLARDGVKSLMLFGISHHKDASGSDAWNRDGLV
                     ARMVRKAKRAAPHLVVIPDVCFCEYTDHGHCGVIEHGHVANDITIENLARQAVVAADA
                     GADLVAPSAMMDGQIAAIRHALDAAGHTDTPIMAYSTKFASAFYGPFRVAAGCELQGD
                     RKAYQMDPMNGREAIRESLQDEAEGADMLMVKPGIAYLDVLARLRERTLLPIAVYQVS
                     GEYAMIKFAAAAGALNEPVVVRETLGAFKRAGADLILSYFARDVIREGF"
     gene            5774..>6614
                     /locus_tag="VKQ27_08595"
     CDS             5774..>6614
                     /locus_tag="VKQ27_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89029.1"
                     /translation="MFWSSLIDLLFPPDPGGLRLLAALRATLAAVIAFFAVVLLGLCV
                     ELPLSDRIVGFAVALFIAANVRDPTPRARLVTIAIAPFVAFASSVLATLLLPWPIASA
                     AIVPPLMFAIVYCASRGPRYASLGIVGLIIYFISLVARQPPETLPMRVVVLLTATAAV
                     VLVRGVLMREQPQVELERLRRAVHASVDRVLHRIARALDSGAWTDASRAVLRREVSRL
                     SETVMLAQARVAALGQQVPQQGSRWLYLLTIELATERVARAALQDLGGAEDRVELHTL
                     LNAL"
BASE COUNT         1149 a         2138 c         2101 g         1226 t
ORIGIN      
        1 cgaccgaccg gccaggtcac cggggccgta tccagcgcca gggtggacgc gacttcatcc
       61 agcaattcga tcggatcgcg gctctcgcgg tccatcttat tgatgaaggt aacgatgggg
      121 atgtcgcgca ggcggcagat ctcgaacagt tttcgcgtcc gcgcctcgat gcccttggca
      181 gcgtcgatca ccatcacggc cgaatcgacc gcggtcagcg tgcgataggt gtcctcggag
      241 aagtcctcgt ggccgggagt atcgagcagg ttgaacacgc agccggcatg ctcgaaggtc
      301 atcaccgatg tgaccacgga gatgccgcgg tcgcgctcta tgcccatcca gtcggaacgg
      361 gtgcgccggc gctcgccctt ggcccggaca ttgccggcca gctggatcgc cccaccagcg
      421 cgcagcagat gctccgtcag cgtggtcttg cccgcgtccg ggtgggagat gatggcgaat
      481 gtgcggcgtc gagcgatttc ggcggcggcg tcagtcatga gcggttccgg gcggccgccc
      541 ggccttcctt ggtcatggcc gggcgtagac ccggccatct ttctggcctc ataaggaagc
      601 gccggggacg gagccccggc gctgtctgca ctctaatata gggatgcggc ggccgataac
      661 agggggatac cgccgttccc ggcccgctac cgcttcacct caggataata ttgcggcgcc
      721 tcgcgccgat cgaacatgcc atagtcgcgc acgacgcgga ccgtgcgatg ccacagctcc
      781 gtctgtccca ccgcgcgagg tcgccaggcg tccgccgcgc ccacatcgcg ccatgacgcc
      841 agcagcagca gtttgccggc aacgtagata ctttcgtagg tatcgcggtc caacagaccc
      901 tgcgtgcccg atgcaggcag atcaaggtcg cggacgaggt cgtccgtcgc aggcttgccg
      961 tcacgcggac ccagctcgct gatcgtcacg tacctcgccg cccccacttc ggtctcgtcc
     1021 aaacgctgct gccgcagcac ctgccccgcc ggcggacggc tatccgcggt gaactcaccg
     1081 acccgcagat ggtagtcctg gaacacctcg aagcggccct tctcctgcac ctgatgatgt
     1141 accgcgagcg tccgccagcg gatcaccgac ttctcgtccc gccaagttga cagcgacagc
     1201 acccgccccg gcacgctccg gctggcgaag cgctcgttat cgaggaagcc gtcgatcttc
     1261 tcgatttctg ggcgcaggaa cttcgcgagc tccaggtatt cgtccttgcg ctcggtcttt
     1321 ggcacgactt caaaaatcac agcaaacatg tggatctcct tcacgccatc ggctggtcat
     1381 caccacggag gaatgcctcg gtctcgcgat acagagcgta gcggccttcc tccagatgca
     1441 tcagatgcgt ggcacggctg atcttcacgt cacggcgcag cggcgaggcg cgcaaggcat
     1501 cgaacagcca gcgcgcatcc gcgtcagtgg ccaaactgtc ccactcgccg cggatgatcg
     1561 agaccggcgc actgatcagg ccaggatcat aggcgaggcg accggcccag gcatcggcga
     1621 tgtcctgcca cggcccactc ggtatcctga cgctgggcgg cgtgcgcacg cggctctccg
     1681 gatcagtgtc caggtagagc ggtccccact catcgaaatg gcgccgcgac agcacggacg
     1741 gctcacccgc gggcacctcg gcggtgaagc gcgtccactg gtcctctacc gaaaccaacc
     1801 gccaggccgg ataacgctgc gcttcggccg tgggcgccct ccaggtcaag ggaccgaaga
     1861 acaccagccg gtcgaccaga tccgggcagt caccggcgaa ccggccagtc gcgatcgttc
     1921 cccatgagtg cgcgatgatc gagatgcgtg ggacggtgtg atgcgcacag atccagcgcg
     1981 ctgcacattc gatctggcga ctggccatct cggcggaacc gagcacgccg tgacactcag
     2041 ccggctcggc catctccgga tagcggtcgg acgcgccgaa gccgacaaaa tccaatcccc
     2101 acacgtcaaa acctcgctca caaagctcat cacgccacga gcggccatcg aagcggtgag
     2161 cgatcgaaag agcggaaggg aacgtgccgc cgtggacata gaggacgacc ttcccccgag
     2221 ccgcgggttg ctttggcgaa aggttgcgca ggaatagatt gagtccgctg acggtactcg
     2281 gaatccaaaa gtgctgctcg cgttcgtcaa gcttggtggc ctgcatctgg aatctcctgt
     2341 gtccgatgca tgcaacgtag gcaggcattg cctgttgcat cgagcgggaa cgcttcggcc
     2401 gatgccgtaa gatggagcgg ccgtgtgttg ctgtcccttc atcttcagcg catgctcttg
     2461 cgggcaagag cctgcccgga gaacgccatg gataccgcga tccctccatc aacgctcgct
     2521 gctcgatcat ggcccgatgg tggcacctgg cgtgtgccga actgggtcta taccgaccca
     2581 gtcgtcttcc agcgcgagat ggaacggatc tttagcgcga gcgattggct gtatgtatgc
     2641 ctcgaggcgg aactgccaaa acccggcgat ttcagacgca gccagcttgg caatcgcgag
     2701 gtcattgcag tccgtggtgc cgatggcgcg atcaacgtgc tcgtcaaccg atgttcgcat
     2761 cgcagcatgc aggtctgcat ggcgagccgt ggttcggcga aggaatttgt ctgcccctat
     2821 catcaatgga catatgacct tgccggcaac ctgctcgggg tcccgttccg ccgtggctat
     2881 cgtgggcaag gaggcatgcc cgacgatttc caacccgagc agcacggatt gcagcgcctc
     2941 gccgtcacga cgcggcacgg ggtcgtgttc gcaagcttcg gcgcacccga tgaatcgctt
     3001 gagacgtatc tgggcgaccg catgctgggc tatttcgacc gcgtgttcga cgggcgcgac
     3061 ctgattgttc tgggttacat gcgccagcgc atccccagca actggaagct gatgttcgag
     3121 aacatcaagg atccctacca cgccagtctg ctgcacgtgt ttctggtgac gttcggcctg
     3181 ttccgcctgg accagcaatc cgccgttgaa atggacgaca cgggacgtca tgccgttctg
     3241 gtgagtcggc gcggcgcgca ggagagcaac gaagcgacca gtcagatgcg cgccttcagg
     3301 cagaacttcg cgctgcgcga caagcggttg ctggacccgg tgcgagagtt tccgggcgat
     3361 gcaactgttg tcctgcagac attgtggcca aacctgatcg tgcagcagca gtcgaatacg
     3421 ttggcgatgc gccagatcgt gccgctggac gcacgcagct tcgatctggc gtggacgttc
     3481 ttcggctatg ccgatgacac gccggagatg cgacaacgcc gcctgcggca ggccaatctg
     3541 atgggaccgg cgggcctggt ctcgctcgac gacagcgagg cgatgctgct gtcgcaggcc
     3601 ggcatcgacc aggatgacgg cgcggcctgc ctggtcgaga tgggcggccg ggcggtgggc
     3661 aacgaaccgc atatggtcac cgagaccgcg attcgcggtt tctatcagca ctaccgggca
     3721 gtcatgggcc tatgatcgcg gtcgcgtacg ggctgacggc tggttgcgag ctgagagcgc
     3781 gtctcgctga cctgtattgt gcctacgaca acgcgctgaa cgatggcgag ctggagcaat
     3841 ggcccgcgtt gttcgcggag gattgcatct acaaggtgat cccgcgcgaa aacgtcgagc
     3901 agggattgcc ggtagcggtg atctattgcg agagccggga catgctgact gatcgcgtgg
     3961 tcgcactgcg cgagacggca ctgttcgcgc cgcgtattgc ccgcaggatg accaccgcca
     4021 tctgcctgcg tacaatcgac ggtgacggca tgcacctcac cgccagcttt gccgtcttcc
     4081 agaccatgcc cgatcgaccg agcgaactct tcctctgcgg cagctgccgc gatcgcgtga
     4141 tggacgacgc cggcacgctg cgcttcgccg agcgtgtgtt tatctacgac tcaacgatcg
     4201 tgccgacgtc actggttcat ccgttatgat gccctcttga tagcgagggc atcataacga
     4261 ctcactactg catcgtcgac agcgccgtcg gcaccatgcg cgtgatgatc atcttctcgc
     4321 ggtccagcat ctccggcacg cgcggcgcga tcttggtctt ccaatagtcg gtatcgtaga
     4381 ccgccttgta ctgcgcctca cgctcggcct cgctgttgaa gcggcgcagc cagatgtaca
     4441 cggactcatc ggtttcgcca cggaagctgc cgcagatcac catgcccttg gagacctgga
     4501 aggggatgat ctcctcctcc atgaccttca cccactcggc ctgcctgccg gggcgcacgt
     4561 ggtattggcg aagctcgtaa aaggccatcg aaacctccct gatgatagag aaccgaccat
     4621 accctgcggc cttcgggctt gcgaatccgg acaggttggc cgggtggtga tcggcgcgtg
     4681 agaacagggc tggtcgggct gcgcgcccaa cccgttgatt atcggggcct gaacgcgcaa
     4741 atcttgggcc tggatcggag actgccatgg cctcaccagc atttcccacg acccgtctgc
     4801 gccgtctgcg ccggtcggaa cccctgcgtg cactggtgcg cgagaccacg gtcaccgcga
     4861 acgacctgat ccagcccatc ttcgtcgagg aaggcatcga cgaagcgctg ccgatcgcag
     4921 agatgccggg cgtgtcccgc atcccggagc gcaagatcga gcacgtcgtc gaaggcctcg
     4981 cgcgcgatgg ggtgaagtcg ctgatgctgt tcggcatctc gcatcacaag gacgcctcgg
     5041 gcagcgatgc ctggaaccgc gacggcctgg tggcgcgcat ggtgcgcaag gcgaagcggg
     5101 cggcgcccca cctcgtggtg attcccgacg tctgcttctg cgaatacacc gaccacggcc
     5161 attgcggggt gatcgagcat ggccacgtgg ccaacgacat caccattgag aatctggcgc
     5221 gccaggctgt cgtcgcggct gacgccggcg ccgatttggt tgcgccctcg gcgatgatgg
     5281 atggtcagat cgccgcgatc cggcacgcgt tggatgctgc cggacacacc gacacgccga
     5341 tcatggcgta ctccaccaag ttcgcatcgg ccttctacgg cccgttccgc gttgcggccg
     5401 gttgcgagct gcaaggcgac cgcaaggcct accagatgga cccgatgaac ggccgcgagg
     5461 ccatccggga gtcgctgcag gatgaagccg aaggcgccga catgctaatg gtcaagcccg
     5521 gcatcgccta tctggatgta ttggcgcgcc tgcgcgaacg gacgctgttg cccatcgccg
     5581 tctaccaggt gagcggtgaa tacgcgatga tcaagttcgc tgcggcggcc ggcgcgctga
     5641 acgagccggt cgtggtgcgc gagacgctcg gggcattcaa gcgtgcgggc gccgacttga
     5701 tcctatccta tttcgccaga gacgtgatcc gcgaagggtt ctgatcgtca gcgatcagat
     5761 ctcgagccgg cacgtgttct ggtccagtct gattgacctg ctgtttccgc ccgacccagg
     5821 tgggttgcgg ttgttggctg ccctgcgcgc cacgctggcc gccgtcatcg cgttcttcgc
     5881 ggtggtcctg cttggcctgt gcgtggaact gccgttgagt gatcgcatcg tcgggtttgc
     5941 cgtcgccctg ttcatcgcgg cgaacgtacg cgacccaacg ccacgggcgc ggctggtcac
     6001 catcgcgatc gcgccgttcg tcgcgtttgc ctcgagcgtg ctcgcaacac tgctgctgcc
     6061 atggcctatc gcctcggcgg cgatcgtccc accgctgatg ttcgcaatcg tctattgcgc
     6121 gtcgcggggg ccgcgttacg cctcgctcgg gattgtcggg ttgattatct atttcatcag
     6181 cctggttgcc cgtcagccgc ccgagacact gccaatgcgg gttgtcgtcc tgttgaccgc
     6241 cacggctgct gtcgtgctgg tccgcggtgt gctgatgcgc gagcaaccgc aggtcgaact
     6301 ggagcgcctg cgccgcgccg tccatgcctc ggtcgatcgc gtgctgcacc ggatcgccag
     6361 ggcgcttgac agtggcgcat ggaccgatgc ctctcgcgcg gtccttcgac gcgaggtttc
     6421 ccggcttagc gagacagtca tgctggcaca ggcgcgcgtc gccgcgcttg gccagcaggt
     6481 tccgcaacaa ggcagtcgct ggctgtatct tttgacgatc gagttggcca ccgaacgggt
     6541 cgcccgcgcg gcgttgcagg atctcggcgg cgcggaagac cgcgtcgaac tgcacaccct
     6601 gctcaacgcc ttgc
//
LOCUS       DATGEM010000275         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_500430, whole genome shotgun sequence.
ACCESSION   DATGEM010000275 DATGEM010000000
VERSION     DATGEM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500430"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VKQ27_08600"
     CDS             complement(<1..870)
                     /locus_tag="VKQ27_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLY89030.1"
                     /translation="MNPPTGKLDTHRRWSRAESLYFALWVCLAPLSASAKEPVDYQSL
                     SLTQAAQLIREGKITSTELVDALLLQADAARDLNAFITLDSEAALNAAHAADAAQKQG
                     KPLGPLHGVPLVIKDNIHVAGMPNTAGTEALRNFIPRENARVVEALIAAGAIVLGKTN
                     MHELAFGATSNNATFGTVRNPYDPTRFPGGSSGGTAAAIAAGIAPGGLGTDTGGSVRI
                     PAALTGLVGLRPTSGRYSLEGVTPFSHTRDTVGPLARNVADVVLLDQVISGDRSSVQP
                     ADMKSVRLGVARDP"
     gene            complement(893..1828)
                     /locus_tag="VKQ27_08605"
     CDS             complement(893..1828)
                     /locus_tag="VKQ27_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY89031.1"
                     /translation="MPELILHHYPMSPFSEKIRLVLGYKKLAWKSVIIPRMMPKPDVV
                     ALTGGYRRTPLLQIGADIYCDTALICDVLEHRQPSPSLHPAGSNGGDRIVAQWADSIL
                     FWAAMGYSFSPAGAAFIFQGDAPEVAKAFGEDRAKMRGGASRMHPGDATSAYKSYLRR
                     IASMVDNQPYVIGQQPSLADFSCYHPLWFTRRIAPLAGIFDATPSLSAWMDRMAAIGQ
                     GSMQHSDALAAIAVAAQSEPASVEKDVFQDDHGIALGSRVTITAESFGLEPTEGELIA
                     ATRTRYSLRRSDARAGTVHVHFPRVGYQLRAVKPA"
     gene            complement(2235..>3453)
                     /locus_tag="VKQ27_08610"
     CDS             complement(2235..>3453)
                     /locus_tag="VKQ27_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLY89032.1"
                     /translation="VLAGCASAPQQAPPLRTPEPAESAAQPAPATANPAAVEQSLQPP
                     AVPTRAKATGTPEDARRHIVRGAAAIEMAKNADDLAEAADEFRKATEIAPDMAPAWYN
                     LGAVLAKTGKVDEAIASYRRYLALASDAADAQKVADEIIKLEFHQERQAKEQASSGVW
                     MESDGTPYSMQVVGNKWRLSTTVRPFQPDVEYRYGPGLGSIGMPISDMEQIAFNLELR
                     NNKLAGTWQRAETQVDKCTVPAETGDVQGELDAANGTVTLQFTKARYKTLTAAPFVLS
                     FETKDTCRDVSVLERRNVRFVFRGPLPAGGIGVYVTTAHGLMSSKDWIGELRVTGTVK
                     GSPAELAGLYSADMLIAIDGVPVTTLSAGEAIFRLRGAPGSDVRLTIQRPKAKEPVTV
                     TLRRQALPAVSGT"
BASE COUNT          562 a         1094 c         1133 g          664 t
ORIGIN      
        1 cgggtctctg gccacgccga ggcgaacgct tttcatgtcg gccggttgca ccgaagacct
       61 gtccccggag attacctgat ccaacagcac gacatcggca acattgcgtg caagcgggcc
      121 aaccgtatcg cgcgtatgcg aaaacggcgt gacgccctcg aggctgtatc gtccggaggt
      181 tggacgaagc cccacgagac ccgtcagcgc ggcggggatg cgtacggaac cgccagtatc
      241 ggttccgagt cctccaggcg cgattcccgc cgcgatcgcg gctgcggtgc cgccgctcga
      301 ccccccgggg aagcgcgtgg ggtcgtaggg attgcgaacc gtgccaaagg tcgcgttgtt
      361 gctggtcgcg ccaaaggcca attcatgcat gttggtcttg cccagtacga tcgcacccgc
      421 agcgatcaag gcttcaacca cccgcgcatt ttcccgtggg atgaagttgc gcagcgcttc
      481 ggtgcccgcc gtgttcggca tgcccgccac atgaatattg tccttgatca cgagcggcac
      541 gccgtgcagc ggtcccagag gtttgccttg cttttgcgcg gcgtcggcgg cgtgagctgc
      601 gttgagcgcg gcttcgctat ccagggtaat aaatgcattg agatcgcgtg ccgcgtcggc
      661 ttgcagcaac aatgcgtcga ccagctctgt actggtgatc ttgccttcgc ggataagttg
      721 tgcagcttgc gtgagcgata gactttggta gtccaccggt tccttcgcgc ttgcgctcaa
      781 aggtgccagg caaacccata gcgcgaaata caatgattca gcacgcgacc atcggcgatg
      841 cgtgtcgagc tttccggttg gcggattcat tttgaatgat gctgcgcgat ggtcatgctg
      901 gcttcactgc ccttagctgg tagccgacac gtggaaagtg cacatggacc gttccggccc
      961 gcgcgtcgct gcggcgcagg ctgtaacgcg tgcgggtggc agcgatcaat tcgccttcgg
     1021 tcggctccag gccgaagctt tcagcggtga tggtgacgcg gcttccgagc gcgatgccat
     1081 gatcgtcctg aaatacatct ttctcgacgc tggccggctc ggattgcgcc gccacggcga
     1141 tcgcggccag ggcgtcggag tgctgcatgc tgccctgccc gatggctgcc atgcggtcca
     1201 tccaggcgga gaggctgggc gtggcatcga aaatgccggc cagtggcgca atgcgcctgg
     1261 taaaccagag cgggtggtag caggagaaat cggcgaggct gggctgctgg ccaatcacat
     1321 agggttggtt atccaccatg ctcgcaatcc ggcgcagata cgacttgtag gctgaggttg
     1381 catcgcccgg gtgcatgcga ctcgccccgc cgcgcatctt ggcgcgatct tcgccgaacg
     1441 ccttggcgac ttcgggtgcg tcaccctgga aaatgaatgc ggcaccggcc gggctgaagc
     1501 tatagcccat cgccgcccag aacaatatgg agtccgccca ctgcgcgacg atgcgatccc
     1561 cgccattgct gcctgccgga tggaggctgg gcgagggctg gcgatgttcc agcacgtcgc
     1621 aaatgagcgc cgtgtcgcaa taaatgtccg ctccgatttg cagcaatgga gtgcgccggt
     1681 agccgcccgt cagcgcgacc acatcgggct tgggcatcat gcgcgggatg atgaccgact
     1741 tccatgccag ttttttgtag ccgaggacca ggcggatttt ttcggaaaac ggcgacatgg
     1801 ggtaatggtg gaggatcaac tcgggcacgg cgggtccttt cagcgtatgg ctggcatgcg
     1861 cgcgcagaaa tctacgcgct ggactgcgcg gcaaaccggc cccagcgcag gcctgaggtt
     1921 gggaacttta ccacttgtca tcggcggcgc atcgacacct gcgccacccc gatgccgggc
     1981 ctgtccgggg gcggattccc tcggggcttt gctttttttc gacggcttcg acttgcagga
     2041 actgttgagg gcgaccgact ggcgaacgag tgccttgaac gcggactcgt caagctcttt
     2101 tccttcgtgg atgtcgattg cgcggcgaac gttcatctat aaagtatagg cctgacccta
     2161 agtgtcccga gtgaacgcag gcccacccgc accgccgcgc ttgcacccgc cggttcagtt
     2221 gtgccaggcc gcgttcaggt tccggacacg gcgggcagtg cctggcggcg cagggtcacg
     2281 gtgaccggct ccttggcctt gggtcgctgg atggtcagcc gcacgtcgct gccgggcgca
     2341 ccacgcaggc ggaagatggc ctcgccggcc gacagggtcg tgaccggcac accgtcgatg
     2401 gcgatcagca tgtccgcgct gtacagtccg gccagctccg ccggcgaacc cttgacggtg
     2461 cccgtgacgc gcaactcgcc gatccagtcc ttcgagctca tcaggccatg cgccgtcgtg
     2521 acgtagacac cgataccgcc cgcgggcagg ggaccgcgga agacgaagcg cacgttgcgc
     2581 cgctccagca cgctcacgtc acggcaggtg tccttcgttt caaaggataa gacgaaaggc
     2641 gccgccgtga gggtcttgta gcgcgccttg gtgaactgca aggtcaccgt accattggcc
     2701 gcgtccagct cgccttgcac atcccccgtc tccgccggga ccgtgcactt gtcgacctgg
     2761 gtctcggcgc gctgccaggt tccggccagc ttgttgttgc gcaattccag gttgaaggcg
     2821 atttgctcca tatcgctgat gggcattcct atcgaaccga ggcccggacc gtagcggtat
     2881 tccacatcgg gctggaaggg ccggaccgtc gtggacaggc gccacttgtt gccgaccacc
     2941 tgcatcgagt aaggcgtgcc gtcgctctcc atccagacgc cgctgctggc ttgctccttg
     3001 gcctggcgct cctggtggaa ctccagcttg atgatctcgt cggccacctt ttgtgcgtcg
     3061 gcagcgtccg acgcgagggc caggtagcgc cggtagctgg caatggcctc gtcgaccttg
     3121 ccggtcttgg ccagcaccgc gcccaggttg taccaagccg gcgccatgtc cggcgcgatt
     3181 tcggtggcct tgcgaaactc gtcggcagcc tcggccaggt cgtccgcgtt cttggccatc
     3241 tcaatggccg ccgcgccgcg cacgatgtgg cgacgggcat cctcgggcgt gcccgtggcc
     3301 ttcgcgcgcg tgggcaccgc tggcggttgc aaggactgct cgacggcagc cggattcgcc
     3361 gtcgcagggg cgggctgggc cgcactctcg gccggttcgg gcgtgcgcaa cggcggcgcc
     3421 tgctgcggcg ccgaagcaca gcctgccagc acg
//
LOCUS       DATGEM010000276         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_207096, whole genome shotgun sequence.
ACCESSION   DATGEM010000276 DATGEM010000000
VERSION     DATGEM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207096"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VKQ27_08615"
     CDS             complement(<1..341)
                     /locus_tag="VKQ27_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY89033.1"
                     /translation="MHVLFGLVGGGNFELARTFGDIGGRYVGLRHELNAAAAGDAYSR
                     TTRSIGAVTLTQGPGLTHAVTAIFEAVKSGTPMLILAADTADQLANQAVNQAALAAAA
                     GAGFERWDQPQV"
     gene            complement(750..2210)
                     /locus_tag="VKQ27_08620"
     CDS             complement(750..2210)
                     /locus_tag="VKQ27_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HLY89034.1"
                     /translation="MLRNARKTDASAAGGPAPPDLKRGKLGTLSLLGQSVAGVGPSIG
                     AAAFFTLAFADAGNATWISVILAVIGVLAVGVCISYLARHHVSPGALYAFIPRGLRSG
                     GVGFLGAGIALTLGACATIGALFGFGLYAANFLSEASIANLSAGQIAYLDLVFLVIAG
                     LLAVLDIRISTRTLLVIEVLSMAAISSLLIVVLARHPGGAFNSAVATGRGSSIHGIVL
                     GAVFFILGFGGFESAAALGAEARRPRRSVPLAVLGSVLVVALFFVVNAYVQVLGFTGT
                     SQSLAAQAFPLTALAKLYHVGWLGTLVALGVAISFFSALNAFINYTARVAYAIARDRL
                     LPRHLGRVHPRTGAPHVAVAALTGMAAIALVIFTVAHADQATVFGDVSTIAGYCFTLL
                     YLLVSLAAIGWILQQLTRPAAVIAAGVIGAAAMAVEFYYSFVPFPAYPASLPLIIFIC
                     ILGALLITYIIVRITLAEVAARALRGTTLIEDDDFA"
     gene            complement(2383..>2703)
                     /locus_tag="VKQ27_08625"
     CDS             complement(2383..>2703)
                     /locus_tag="VKQ27_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89035.1"
                     /translation="QAEQLIGGRGAIAHGRDEVEAAIAQTRDFLQIMLHEVGTVHRRG
                     GTLKEAFQAAHAALAPTYSKWPIFEHCAPFDVQRVWDELNGIDWPHIWTQERDQEVWD
                     QLQD"
BASE COUNT          528 a          840 c          901 g          434 t
ORIGIN      
        1 acctgcggct ggtcccaccg ctcgaagccg gctccggcgg ccgcggccag tgccgcctgg
       61 ttcaccgcct ggttggccag ctggtcggcg gtgtcggccg cgaggatcag catcggcgtg
      121 ccggacttta cggcctcgaa gatcgctgtg actgcatgtg tgagccctgg tccctgggta
      181 agcgtgacag ctccgatcga ccgcgtggtg cgcgagtagg catcgcccgc agcagcggcg
      241 ttcagctcat gccgcagccc gacataccgt ccgccgatat caccgaatgt cctggccagc
      301 tcgaagttcc cgccgcctac gagcccgaac aggacatgca cgccactgtc tcgtagggcg
      361 tgcgcgagat cttgttgcgc cgtgttcacg agcggccccg gttacgccgg ccggggagcc
      421 tgccagggcg atccaccagg cacatcgcag caccacctcg cagacagccc ggcgcggccg
      481 ggcgacggtc caggtcgaca ctacgtcctg aatggagtgc catacagtcc atatcttcgg
      541 ctaaacgcca gtccaattga ttccgtgctt gctgcctgag ccgcttcgga tcactgatga
      601 caccggcggg gtgcgtgcac agcgaccggc gcggtgcaga atcggtgctg tgactcgtac
      661 ggcagagcgt cagcgccgcc gcgcatttca cgatcgtgga gggcgatcag gcgatcgcga
      721 tcgcgatcgc ggagccgacc tggagggggt taagcgaagt catcatcttc gatgagcgtc
      781 gtgccccgca gagcacgtgc ggccacctcg gccaaagtga tccggacaat gatgtaggta
      841 atcaggagtg cgccgaggat acatatgaag atgatcagcg gaaggcttgc cgggtaggcc
      901 gggaacggca cgaacgagta gtagaactca accgccatcg ccgccgctcc gatcacaccg
      961 gcagcgatga cagcagccgg cctggttagc tgctgaagga tccatccgat ggcagcaagc
     1021 gagaccagaa gataaagcaa ggtgaagcag tagcccgcta tcgtactgac gtcaccgaag
     1081 accgtggcct gatccgcatg cgccaccgtg aagatcacca aggcgatggc tgccattccg
     1141 gtgagcgctg ccacggcgac gtgcggagcg cccgtacgcg ggtggacccg gcccagatgc
     1201 ctcggaagca ggcggtcgcg ggcgattgca tatgccacgc gggctgtgta gttgatgaat
     1261 gcattgagtg cggagaagaa gctgatggcg acgcccagcg cgaccagggt tcccagccac
     1321 cctacgtggt acagcttcgc caaggctgtc agcgggaagg cctgcgcagc tagcgattgg
     1381 ctggtacctg tgaatcccag cacctggaca taagcattga ccacgaagaa gagggcaaca
     1441 accagcacgc tgcccagcac tgcgagcggg acgctgcggc gcggccgacg tgcctcagca
     1501 ccaagagccg ccgcgctttc gaagccgccg aacccgagaa tgaagaacac cgcccccagg
     1561 acgatgccgt ggatgcttga cccccgccct gttgcgacgg cggaattgaa agcgccgcca
     1621 gggtgacgcg cgagaacgac gatcagtagc gatgagatcg ccgccatgga cagtacttcg
     1681 attaccagca gagtccgcgt ggagatccgg atgtcgagga cggcgagaag ccctgcaatc
     1741 acgaggaaca ccaggtctag atacgcgatc tggcctgcgg acaggttcgc gatactggcc
     1801 tcggaaagga aattcgcggc atacaagccg aagccgaata gtgcccctat cgtggcgcag
     1861 gcaccaagcg tgagcgcgat gccagctccg aggaagccga cgccgccgga cctcagacct
     1921 ctcgggatga acgcgtagag ggcacccggt gagacgtgat ggcgcgctag gtaggaaatg
     1981 cagacgccga cggcaagtac gccaatgacg gccaggatga ccgaaatcca ggttgcgttc
     2041 ccggcgtcgg cgaaggccag cgtgaagaat gctgcagcac cgatgctggg accgacaccg
     2101 gccacagact ggcccagcag cgaaagagta ccgagcttgc cgcgcttcag atctggcggg
     2161 gcaggccccc ctgccgccga ggcgtctgtc ttgcgcgcat tcctgagcat gggatacctc
     2221 cagggattcc tcttcgggga tgggtggcgt tatcacgttc gggccgtgac cgctgtttac
     2281 gattgggtgt tgccggcgat ccggcacact gactgggttc acgctgggcc gcaggtcagg
     2341 accggtggca tcacggtacg gacaggccac tccggaatca ggtcagtctt gcagctggtc
     2401 ccacacttcc tggtcgcgtt cctgcgtcca gatatggggc cagtcgatgc cgttgagctc
     2461 gtcccacacg cgttgcacgt cgaacggggc acaatgctcg aagatcggcc atttgctgta
     2521 cgtgggggcg agcgcggcgt gcgcggcttg gaatgcctct ttcagggtgc cgccgcggcg
     2581 gtggacagtc ccgacctcgt gcagcatgat ctgcaggaag tcacgggtct gggcgatggc
     2641 agcctcgacc tcgtcgcgtc cgtgcgcgat ggcgccgcgg ccgccgatga gctgctcggc
     2701 ctg
//
LOCUS       DATGEM010000277         9142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_591204, whole genome shotgun sequence.
ACCESSION   DATGEM010000277 DATGEM010000000
VERSION     DATGEM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591204"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /gene="tssM"
                     /locus_tag="VKQ27_08630"
     CDS             complement(<1..2426)
                     /gene="tssM"
                     /locus_tag="VKQ27_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HLY89036.1"
                     /translation="MARTIALLTSRWCISFLGVALLAGVAWFFAPLVPGFEDWPPRVA
                     VVMALLMAWAGANAMLDLRRLHREAALAKGIAAGAERAEEAQALRERLTAGLDLLKRS
                     SRSRSYLYEHPWYAIIGPPGAGKTTALLNAGLRFPLAEQMGQGAIAGVGGTRLCDWWF
                     TEDAVLIDTAGRYTTQDSNADVDRAGWDAFLGLLKQTRPKQPLNGLIIAFPIGDIANG
                     RAAERKAHADAIRSRIEELQARFGIRMPVYLLFTKVDLVAGFTEFFDDLDREQRAQVW
                     GTTFDLTDGRDSVVELFTGELRVLIEQLNARLFNRMQAERDVERRARIAMFPGQMASL
                     EGLLADFLHMVFDEKRGGSTPQLRGVYFTSGTQEGTPFDRLTGTLARALGVDQGGTQS
                     LRPVQGRSYFLERLLKDVIFGEASLVAHSPAATRRRFALRIAGYAVAALLVIASGTAL
                     LWAHLAGQRAIAASSTALANYEQTAHGLTLDPVADDDLPRLVPLLDQARALPRGNQEP
                     SWLPPLLSQREKLSASAQTVYRHALQFALLPRLMWRLETQLRGNLNREEFLYEATRVY
                     LMLGNAGPLDVPLVTAWMKLDWETAYPGLGYAPLRDSLAQHLSALLAEPLPQMQLDGA
                     LLSQVRARIATVPLAQRVYSRIRPSAAAQHVPPWRPGDVLGVAGATLFMRSSGKPLSD
                     GIPGFLTTDGFHKVLLPSLDGAVKGVVSESWVLGSRVAFDPNGPQVPALEREVIGLYE
                     RDYAQAWDQMLADLNPVQLRSLPQAAQDLYILAASDSPMHSLLASIARQLTLSAVPAE
                     TQSGNPQAGAT"
     gene            complement(2433..3851)
                     /gene="icmH"
                     /locus_tag="VKQ27_08635"
     CDS             complement(2433..3851)
                     /gene="icmH"
                     /locus_tag="VKQ27_08635"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HLY89037.1"
                     /translation="MSDNPFQEPDDDRTVIRPAPGGRRPAPPAGTAPPRVPPATHDAR
                     PAATPAPAPPHLPPTTRDTRPASIPAVDPSLPPAFSVSPLAAAASPLLQLLNHLRQLR
                     QPPDLQGVRDRCLQDLRTFERQARDASIAMEHLRPAHFALCASLDDVVLNSPWGAGSD
                     WSKQTLVANQHPTVRDPDHFFLELRQMLAAPQRYLPAIEVMYLCLSLGYMGRYRRERG
                     GGELADVRAAAHAAIAAQLPAPAPELSRRWRGVVVPYQLGNRGVPVWVAMAAAVALCG
                     SLLLWASANLNAASDVVQAQALAAPPTLMPQITRAAAVQPVPSPPAPPEPSALDRLRA
                     ALQPDIDAHAVILLGTPAAPVVRLFDRTMFRPGTATVSTASVPMLERVSAALGKEPGT
                     VRVIDYADNQPVHTVRFPSSFQLSAARAEAVRAIVARKIGDPARVVAEGRGDADPIAS
                     NATAEGREQNQRIEIVLQQRQD"
     gene            complement(3862..4638)
                     /locus_tag="VKQ27_08640"
     CDS             complement(3862..4638)
                     /locus_tag="VKQ27_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HLY89038.1"
                     /translation="MGLVPSPTVLSVDQMGRIAPPIFTSVGRGAVNAPGDVFVVQSLL
                     NDRLPKPHAPVPVNGVADIGTTLAIETYQAVFLAMMPPTGRIDPGSATYYSLAARPLV
                     EGAPPPSAGHYGQVPTEVADAAIGSRKKWQVPASVTVAQWAVESAWGASMPPGSNNPF
                     GIKAVGDQPAVDSPTREVINGESVIITARFRAFTSLSEAFDDHGRLLATAPVYKDAMK
                     EANNPQAFADALTGVYATDPKYGFTLNWVIENYGLSQYDR"
     gene            complement(4892..6256)
                     /locus_tag="VKQ27_08645"
     CDS             complement(4892..6256)
                     /locus_tag="VKQ27_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY89039.1"
                     /translation="MDEPNAAELFAAHVAGVHYADIPPTAIERAKVFILDSLGVGIAG
                     STAPGRSEILAAARRWGAGDEATLWGGGGRLPAPAATMLNAFQMHCQEFDCVCEPAVL
                     HPMATLLPAALAYAEREGGISGKALLTSVAVGVDVSGWIGIASTQPLRFFRPATAGGF
                     GAAAAVARLAGLDTEGIVHAFGLLYTQTSGTMQAHVEAGPALPLQVGFNSRAALQAVD
                     LALTGLPGPLGSIDGPFGYLPLMEGGYTLDQVRAELGRTWLVAGLAHKPYPAGRATHG
                     AIEGVAVLRERHGFAADDVDEIVVTVPPLTGQLISRPDLPDPLPNYARLCTAFVVAKV
                     LQHGHLDLSHYRGTELTDERTHQLAANVRVQTDSNPDPNALVPQRLVVRLRDGRELHW
                     ACDSMLASAERPLTREQHLAKFRRCCAFADRPWHDAEIDALIACVDRLETLADVRVLT
                     KILG"
     gene            complement(6264..7343)
                     /gene="rutA"
                     /locus_tag="VKQ27_08650"
     CDS             complement(6264..7343)
                     /gene="rutA"
                     /locus_tag="VKQ27_08650"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HLY89040.1"
                     /translation="MDLGVFIPIGSNGWLISKTSPRYRPTFALNRDTTQLAERYGFEF
                     ALSMVKLRGFGGESEFWDHCLESFTLMAAIAAVTSRIRLYASTAVLTLPPAIVARMAS
                     TIDSVAPGRFGINIVSGWQGAEYSQMGLWPGNDHFANRYECSAEYVQIMRELWTTGRS
                     DFKGKYFTMDDCRLSPQPSSMPAIVAAGQSDVGMKFAAEHADFSFCLGAGVNTPTAFA
                     PSVARLVEFAAGTGRKVGAYGLFMVIADETDAKAEAKWELYKEGKDLDALSWMGVQAG
                     ADDKADENSTARSITNPVSAVNFNMGTLVGSYETVAALLDEIATVPGVAGVMLTFDDF
                     IAGMEAFGQRIQPLMQSRKAVLAAA"
     gene            7606..8460
                     /locus_tag="VKQ27_08655"
     CDS             7606..8460
                     /locus_tag="VKQ27_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HLY89041.1"
                     /translation="MRDLLAQPGIITLPGVFDGISARVANSLGFPALYMTGYGVVASA
                     LGVPDAGTATYTEMLDRVRMIASAVRVPFIADGDTGYGGLLNVDRTVRGYAAAGAAGI
                     QIEDQEFPKKCGHTEFRRVIPAQDAVMKIRVAVDARPDKSFLIVARTDARYAHGIDDA
                     LRRAEMFLSAGADVLFVESPESLEEMRRVAETFRGAPLLANMVEGGRTPYLSTSELEA
                     MGFKIALFPGTGFLAAAHALRAAYAHLQSAGTSTGGPPLYPFSEMNKLMGFPSVHAFE
                     AKWTEAGR"
     gene            8457..>9142
                     /locus_tag="VKQ27_08660"
     CDS             8457..>9142
                     /locus_tag="VKQ27_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HLY89042.1"
                     /translation="MTDALGWRHCFGVLGPSTNTVVQPDMEAMRPVGVTNHYSRIFTP
                     NADAVSNETFMSGARVIGGNTLDAVRSVMTCSPDSLVMGMSAVTFFGGMKGADEFQSM
                     LEAESCVKAVVGSHAVAAALRAYGGVRRVAFMSPYFPAANAEVRRYLEDAGFAVVRDR
                     CLQCPSWTAIACIPRETIRDCFRELDGDDVDAIVQVGTNLSAVRLAAAAELWLGKPVI
                     AINAAIYWQAL"
BASE COUNT         1607 a         2822 c         3108 g         1605 t
ORIGIN      
        1 gtggcgccgg cctggggatt gccggattgc gtctcggcag gcaccgcgga gagtgtcagt
       61 tgccttgcga tggaggccag aaggctgtgc atcggcgaat cggaggcggc cagtatatac
      121 agatcctggg ccgcctgcgg caggctgcga agctgcaccg gattcaggtc tgccagcatc
      181 tggtcccatg cctgcgcgta gtctctctcg taaagaccaa tcacttcacg ttcgagcgca
      241 ggcacctgcg gcccgttcgg gtcgaatgcc acgcggctgc cgagcaccca gctttccgaa
      301 accacgccct tcaccgcgcc atcgagcgac ggcagcagca ccttgtggaa cccatcggtt
      361 gtgaggaagc ctggaatgcc atcgctcagc ggcttgcccg atgagcgcat gaacagggtg
      421 gctcctgcga caccgagaac gtcacccggc cgccagggcg gcacgtgctg cgcagccgcg
      481 gatgggcgaa tgcgcgagta gacacgctgt gccagcggca cggttgcgat acgggcgcgt
      541 acctgtgaga gcagcgcgcc gtcaagctgc atttgcggca gaggttctgc cagcaatgca
      601 ctgaggtgct gcgccaggga atcgcgcaat ggtgcatagc cgaggcccgg ataggccgtc
      661 tcccagtcca gcttcatcca tgcagtcacc agcggcacat cgagtgggcc agcgttgccg
      721 agcatgaggt agacgcgcgt tgcctcgtag aggaattcct cgcggttcag gttgccgcgc
      781 agctgcgtct ccagtcgcca catcagccgc ggcagcagcg caaactgcag cgcatggcga
      841 tacaccgtct gagcggacgc gctgagcttc tcccgttgtg acaacagcgg cggcaaccag
      901 gatggttctt gattgccgcg cggcaaggcg cgtgcctgat cgagcaaggg aaccagccgg
      961 ggcaggtcat cgtcagcaac cggatcgagc gtcaggccat gagccgtctg ctcgtagttc
     1021 gccagcgcgg tcgatgaagc cgcgatcgcg cgctggcccg ccaggtgcgc ccacagcaac
     1081 gctgtaccgg acgcgatcac gagcagagcg gcgacggcat agccggcgat ccgcaatgcg
     1141 aaccggcgcc gtgtcgcggc ggggctgtga gcgacgagcg aggcttcgcc gaatatgaca
     1201 tccttcagga ggcgttccag gaagtagctg cgtccctgca ccggccgcag gctctgcgtg
     1261 ccgccctggt ccacaccgag cgcccgcgcc agagtaccgg tcaggcgatc gaatggtgtt
     1321 ccttcctggg tgccggaggt gaagtacaca ccccgcaact gtggcgtcga tccgccgcgc
     1381 ttctcgtcga acaccatgtg aaggaaatct gccaacagtc cttccaggct cgccatctgg
     1441 ccggggaaca ttgcgatacg cgcgcgccgc tcgacatcgc gctccgcctg catccgattg
     1501 aacaggcgcg cattcaactg ctcgatcagt actcgcaact cgcctgtgaa caactccacg
     1561 acactgtcgc gcccatcagt caggtcgaac gtcgttcccc agacctgcgc ccgctgctcg
     1621 cggtcgagat catcgaagaa ttcggtgaag ccggcgacaa gatcgacttt ggtgaagaga
     1681 agatagaccg gcatacgaat gccgaagcgg gcctgtagtt cctcgatccg gctgcggatg
     1741 gcgtccgcgt gcgccttgcg ttctgccgcc cggccgtttg cgatatcgcc aatcgggaaa
     1801 gcgatgatca ggccgttcag cggctgcttc ggcctggtct gcttcagcag gcccaggaag
     1861 gcatcccagc cggcgcggtc gacgtccgcg ttggaatcct gggtggtgta gcggccggcc
     1921 gtatcgatca gcacggcatc ctcggtgaac caccagtcgc ataggcgtgt gcctccgaca
     1981 cccgcgatgg cgccttgacc catctgctcg gccaatggga atcgcaggcc tgcattcagc
     2041 agggccgtgg tcttgccggc accgggcgga ccgatgatgg cgtaccacgg atgttcatac
     2101 aggtagctgc gactgcgcga tgaccgtttc aggaggtcga gccctgcggt gaggcgctct
     2161 cgcaatgcct gtgcttcttc ggcgcgttcc gcgccggcgg cgataccctt ggccaacgca
     2221 gcctcgcgat gaaggcggcg caggtccagc atggcattgg cgccggccca tgccatcagc
     2281 aatgccatca cgaccgcgac gcgcggcggc cagtcttcga accccggaac caacggcgcg
     2341 aagaaccacg ccacgcctgc caacaaggcg acgccgagga agctgatgca ccagcgtgag
     2401 gtcagcaggg caatcgtccg cgccatctgg tgtcagtcct ggcgctgctg caacacgatc
     2461 tcgatgcgct ggttctgctc gcgtccctct gcggtggcgt tggaggcgat cggatcggcg
     2521 tcaccccggc cttccgcgac gaccctggcc gggtcgccga tcttgcgggc gacgattgcg
     2581 cgcaccgctt cggcgcgggc ggccgaaagc tggaaactcg acggaaagcg gacggtgtgg
     2641 accggctgat tgtcggcata gtcgatgaca cggaccgtac cgggctcttt acccagcgcg
     2701 gcagagaccc gttccagcat ggggacggat gcggtcgaaa cggtcgcggt acccggcctg
     2761 aacatcgtcc gatcgaacag gcgaaccacc ggtgcggcag gtgtgccgag caggatgacg
     2821 gcgtgcgcat cgatgtcggg ctgcaacgcc gcgcgcagac gatcgagggc ggaaggttca
     2881 ggcggcgcgg gcggggatgg gacaggctgc acggcggccg cgcgggtgat ttgcggcatg
     2941 agcgtcggtg gcgcggcaag tgcctgtgcc tgcaccacat ccgacgccgc gttgagattg
     3001 gcggaggccc acagcaaaag gctgccgcac agagctaccg ccgcggccat ggcgacccac
     3061 accggcacgc cacggttgcc caattggtac ggtaccacga cgcctcgcca gcgccgcgag
     3121 agctcgggag caggggccgg gagctgagca gcgatcgccg cgtgcgcggc cgctcgcaca
     3181 tccgccagct cgccgcctcc gcgctcccgc cggtagcggc ccatatagcc aagcgatagg
     3241 cagaggtaca tcacttcgat ggccggcagg tagcgctggg gtgctgccag catctgcctg
     3301 agttcaagga agaagtggtc ggggtcgcgc accgttggat gctggttcgc gaccagggtc
     3361 tgcttcgacc agtcgcttcc tgcgccccag ggtgaattca gcacgacgtc gtcgagcgat
     3421 gcgcacaggg caaagtgggc cgggcggagg tgctccatcg cgatgctggc atcgcgcgcc
     3481 tggcgttcga acgtgcgcag gtcctgcagg catcggtcac gcacgccctg caggtcggga
     3541 ggctgccgca attgccgcag atggttcagc agttgcaaca gcggggacgc tgcggccgcg
     3601 agcggcgaga cgctgaacgc cgggggcagg ctgggatcga ctgcgggaat ggaggcaggc
     3661 cgtgtgtcgc gggtcgttgg ggggaggtgc gggggagccg gtgcgggagt ggcggcgggt
     3721 cgcgcgtcgt gggtcgctgg cgggacgcgc ggaggcgcgg tacccgcagg gggagccggg
     3781 cgacggccac cgggagccgg acggatcacc gtccgatcgt catccggttc ctggaacggg
     3841 ttgtcgctca tgggggccag atcaccggtc gtattggctc aggccgtagt tctcaatgac
     3901 ccagttcagg gtaaatccgt attttgggtc tgtcgcatat actccggtca gcgcgtcggc
     3961 gaacgcctgc gggttgttcg cctccttcat tgcatccttg tagacgggag cggtcgccag
     4021 caggcgacca tggtcatcga atgcctccga caacgaagtg aaggcgcgga agcgcgcggt
     4081 aatgatgacg ctctctccgt tgatcacttc gcgggtcggg ctatcgacgg caggctggtc
     4141 gccgacggcc ttgatgccga acggattgtt gctgccgggc ggcatcgagg cgccccaggc
     4201 gctctcaacc gcccattggg cgactgtcac cgatgctggc acttgccact tcttgcgaga
     4261 cccgatggca gcatccgcga cctccgtcgg cacctgaccg taatgcccgg ccgacggtgg
     4321 tggagctccc tcgaccagcg gccgggcggc aagtgaatag taggtggcgc tccccgggtc
     4381 gatgcgcccg gtgggcggca tcatggcaag gaacacggcc tggtaggtct cgatggccaa
     4441 cgtcgtgccg atatcggcca caccgttgac cgggaccggc gcatgcggtt tgggcaaccg
     4501 gtcgttgagc agcgactgga cgacaaagac atcgcctggc gcgttgacgg cgccgcggcc
     4561 aaccgatgtg aatatcggcg gtgcgattcg acccatctga tcgacgctca acaccgttgg
     4621 tgaagggacc agtcccaagg ctgcctcgag ggtatgcgaa tgccgcggtc agattagcgc
     4681 acaagcaggg cgctgaggcc aatgtattga agggtcttcc tgtcggcgct ggcgaaggtc
     4741 tggcgaacgc tgacccagcc agctgagttc acagctgcca agggtctgcg cggtgcagtt
     4801 tgtcggaccg caaaatatgg aaccgtaaaa gacgcaaaaa cacgcggagg gcgtaagcct
     4861 gcggctttca ggcaggtcag gttgccgata ctcacccgag gatcttggtc agcactctta
     4921 catcggccaa tgtttcgagg cgatcgacac aggcgatcag cgcgtcaatc tcggcatcgt
     4981 gccacggccg atcagcgaat gcgcagcagc gacggaattt cgccagatgt tgttctcggg
     5041 tcaatggtcg ctccgccgat gccagcatcg agtcgcatgc ccagtgcagt tcgcggccgt
     5101 cccgcagccg gacgacgagg cgctgtggca ccaacgcatt ggggtcgggg ttgctgtcag
     5161 tttgcacgcg cacgtttgct gcgagctgat gggtgcgctc gtcggtcagt tcggtgccgc
     5221 ggtaatgcga caggtccaga tggccgtgct gcaggacctt ggccaccacg aaggcggtgc
     5281 acagccttgc gtaattcggc agcgggtccg gcagatcagg gcgcgagatc aattgacccg
     5341 tcagcggcgg cacggtcacc acgatctcgt ccacatcatc agcggcaaag ccgtggcgtt
     5401 cgcgaagaac ggccacgcct tcgatcgcgc catgtgtggc tcggccagcg ggatagggtt
     5461 tgtgagcgag gccggcgaca agccaggtgc gccccagctc ggcgcgcacc tggtccagcg
     5521 tatagccgcc ctccatcagc ggcaggtagc cgaatggacc atcaatgctg ccaagcggtc
     5581 ccggcagtcc ggtgagcgcc agatccaccg cctgcaatgc ggcgcggctg ttgaatccaa
     5641 cctggagcgg cagagccggg ccggcttcga catgcgcctg catcgtgccg gaggtctgcg
     5701 tgtaaagcag gccgaaggcg tgcacgatgc cctcggtgtc cagcccggcg aggcgtgcca
     5761 cggctgccgc ggcgccaaag ccgcctgctg tggcaggacg gaaaaagcgg agcggctggg
     5821 tggacgcgat gccaatccag cctgacacgt ccacgccgac tgcgaccgac gtcaggagag
     5881 ccttgccgct gatgccgccc tcgcgctcgg cgtaagccag tgctgccggc agcagggtgg
     5941 ccatcggatg cagtacggcc ggttcgcaaa cgcagtcgaa ctcctggcaa tgcatctgga
     6001 aggcattgag cattgtcgca gccggtgccg gcagcctgcc gccgccaccc cacagggtgg
     6061 cctcatcgcc cgcaccccag cgtcgggctg cagccaggat ctcgctgcgt cccggcgcgg
     6121 tggaaccggc gatcccgaca ccgagactgt ccaggatgaa caccttggcg cgctctatcg
     6181 cagtgggtgg aatatctgcg tagtgcacgc cggcgacgtg cgcggcgaac agctcggcgg
     6241 cgttgggttc gtccatgggg cgctcaggct gcggcgagca cggctttgcg cgactgcatc
     6301 agcggctgga tgcgctggcc gaaggcttcc atgccggcga tgaagtcatc gaatgtcagc
     6361 atgacgccgg caacaccggg gacagttgcg atctcatcca gcagtgctgc gacggtttcg
     6421 tacgagccta ccagcgtgcc catgttgaag ttcaccgctg agactggatt ggtgatggag
     6481 cgggcggtgg aattctcatc ggccttgtcg tcggccccag cctgtacgcc catccagctg
     6541 agtgcgtcca ggtccttgcc ttccttgtag agctcccatt tggcctccgc tttcgcatct
     6601 gtttcgtcag cgatcaccat gaacaggccg taggcgccta ccttgcggcc ggtcccggcg
     6661 gcgaattcga caaggcgcgc caccgagggt gcaaaggcgg taggcgtgtt tacacctgcg
     6721 cccaggcaga agctgaaatc ggcgtgctca gcggcgaatt tcatgccgac atcgctttga
     6781 ccggcggcca cgattgcggg catcgaggac ggctgcgggc tcagccggca atcgtccatg
     6841 gtgaagtact tgcctttgaa gtccgagcgt cctgtcgtcc agagctcacg catgatctgc
     6901 acgtattccg cgctgcactc atatcggttg gcgaagtgat cgttgcctgg ccacaatccc
     6961 atctggctgt actctgctcc ctgccagccg gagacgatgt tgatgccgaa ccgcccgggc
     7021 gcgacgctgt ctatggtgga ggccatgcgg gcgacgatcg ccggtggcag tgtcaatacc
     7081 gctgtagacg cgtaaagcct gatccgtgac gtcaccgcgg cgattgccgc catcagggtg
     7141 aagctttcca ggcaatgatc ccagaactcg ctctcgccgc cgaagccgcg cagtttcacc
     7201 atggagagcg cgaactcgaa gccatatctc tccgccagct gggtggtatc gcgattgagt
     7261 gcgaaggtcg gccggtagcg gggcgaggtc ttcgagatca gccagccgtt gctgccgatg
     7321 ggaatgaaga cgccaaggtc catcgatgtg ctccgatcgc attagcgcag gggatgaggg
     7381 cgtgttgaat ggaggccagc ccctgcatgg tgtggcggcg agtgctgttg ggcaagcgcg
     7441 ttgtttcgaa cggatgtgtg ccaggcgccg ggtgactgcc gcaggcccaa ccggagggaa
     7501 ggttgctatc tcgggagcca ttgttcacaa gacaccgggc gctgcgccgc gggccgcagg
     7561 cgaaccgacg ccattggagg gacggagcca catatcgtga caaccatgcg agacctgctg
     7621 gcgcagcccg gcatcatcac gctgccaggc gtgttcgacg gcatttccgc cagggtggcg
     7681 aacagcctgg gatttccggc cctgtatatg acagggtatg gcgttgttgc gtcggcgctc
     7741 ggcgtgcctg atgctggcac cgccacctat accgagatgc tcgaccgcgt gcgcatgatc
     7801 gcctcggctg tcagagtgcc attcatcgcg gatggcgata cggggtatgg cggcctgctg
     7861 aatgtcgatc ggacagtacg cggctatgcg gctgccggtg ctgcggggat ccagatcgag
     7921 gatcaggaat ttcccaagaa gtgcggccac acggaattcc gtcgggtcat cccggcgcag
     7981 gatgcggtga tgaagatccg cgtggccgtg gatgcgcgtc cggacaagag cttcctcatc
     8041 gtcgcgcgca cggacgcacg ttatgcccac ggcatcgatg atgcgttgcg cagggcggag
     8101 atgttcctgt ccgccggtgc cgacgtgctg ttcgttgaga gtccggaaag cctggaagag
     8161 atgcgacgcg tggcggagac ctttcgtggc gcaccactgc tggcgaacat ggtcgaaggg
     8221 ggacgcacgc cgtatctttc caccagcgaa ctcgaagcca tggggttcaa gatcgccttg
     8281 ttcccaggga ccggtttcct tgctgctgcg cacgcgttgc gtgcggccta tgctcatctg
     8341 caatcggccg gcaccagtac gggcggaccg ccattgtatc cgttctccga gatgaacaag
     8401 ctgatgggct ttccgagtgt gcatgcgttt gaagcgaaat ggaccgaggc ggggcggtga
     8461 ccgacgcact gggctggcgc cattgcttcg gcgtgctggg cccgtccacc aatacggtgg
     8521 tgcagccgga catggaggcc atgcggcctg tcggcgtgac caatcactac agccgtattt
     8581 tcacgccgaa tgctgatgcc gtcagcaacg agaccttcat gtccggtgcg cgcgtgatcg
     8641 gcggtaacac gttggacgcg gtgcgcagtg tgatgacctg ctcgcctgat tcactggtga
     8701 tgggcatgtc ggccgtcaca tttttcggag gcatgaaggg cgcagacgaa tttcaatcca
     8761 tgctggaagc ggaaagctgt gtgaaggcgg tggtcggctc gcatgctgtt gcggctgcct
     8821 tgcgcgcgta tggcggagtg cggcgcgttg ccttcatgtc accatatttc ccagccgcca
     8881 atgccgaggt ccggcgctat ctggaggacg ccggttttgc cgtggtgcgc gaccgctgtc
     8941 tgcaatgtcc aagctggacg gcaatcgctt gcatccccag ggagacgatc cgcgactgct
     9001 tccgcgaact ggatggtgat gatgtcgatg cgatcgtgca ggtgggcacc aacctgagcg
     9061 ctgtccggct tgccgcagca gccgaactgt ggcttggcaa gccggtgatc gcgatcaatg
     9121 ccgccatcta ctggcaggca ct
//
LOCUS       DATGEM010000278         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_354769, whole genome shotgun sequence.
ACCESSION   DATGEM010000278 DATGEM010000000
VERSION     DATGEM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354769"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VKQ27_08665"
     CDS             complement(<1..257)
                     /locus_tag="VKQ27_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89043.1"
                     /translation="MNEQPKLAALGREHSSGFRPPDLVIGHVGFPPLLLQSAVAGDGG
                     WYFSLAIGQLDRPRAHVSVRLRTIADLFASESQSALVGLSS"
     gene            440..817
                     /locus_tag="VKQ27_08670"
     CDS             440..817
                     /locus_tag="VKQ27_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY89044.1"
                     /translation="MTALPRILVVEDDDMVGEILKVMLDECYDVASVAEVRRALAEIA
                     AQPINVVLLDYNLPDACGEDVAECAGRAGVPVVWMTGDLAAADALTAQHHAVLTKPFH
                     TSQLLEALAKAVQGAAVPALRAS"
     gene            1675..>2161
                     /locus_tag="VKQ27_08675"
     CDS             1675..>2161
                     /locus_tag="VKQ27_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HLY89045.1"
                     /translation="MQRWREQYLGLAQFPPAMAAAEVDQFFTLNASDLTAVATRRGPL
                     HRLGVGLQIGFLRMTGRTLNSFQMVPVAVLEHLGAQLGLTVPRLASIRALYRRRRTLF
                     EHQRVAMAALGFRHLTDHAARGLTAHLRRSAEATFSGAALTRLVRVWLHEHRYMLPGD
                     KR"
BASE COUNT          396 a          642 c          716 g          407 t
ORIGIN      
        1 tgggagctca ggccgaccag ggcactctgc gattcgcttg caaataggtc cgcgatcgta
       61 cgaagtcgga cagacacgtg agcgcgcggc cgatctagct gtccgatggc gagggaaaaa
      121 taccagccac catcaccggc aactgctgat tgcagcagaa gcgggggaaa cccaacatga
      181 ccgatcacga gatctggcgg gcggaagccg ctggagtgct cgcggccgag agcagcgagt
      241 ttcggttgct cgttcaagcg ccagaggcgt tcggtggcgc ggtgcggttc ctggttctac
      301 ggcgtggtgc agcgggggag ggagctgaga gccttttcgg gtccggcatc agagacgacg
      361 tgcgcagcgc catgcaagct gccgaacgga tggcggacag ttgggcacgg cttggtctcc
      421 tcgagactga tctatcccga tgaccgcgct gccccgcata ctcgtcgtcg aagacgacga
      481 tatggtcggc gaaatactca aggtgatgct cgatgagtgc tacgacgtgg cgagcgtcgc
      541 tgaggtcagg cgcgcgctcg ccgagatcgc cgctcagccg atcaacgtcg tgctccttga
      601 ctacaatctt cccgacgcgt gcggtgagga cgtcgcagaa tgtgcggggc gtgcaggcgt
      661 gccggtcgtc tggatgaccg gtgatcttgc ggcggcggac gcgctgaccg ctcagcacca
      721 tgccgtgttg acgaagccat tccacaccag ccagttactg gaagcgctcg cgaaagccgt
      781 gcagggggcc gctgtgcccg cgcttcgcgc gagctagatg tagctgcttg ccatcatccg
      841 aggccctgct cgcaaacaga gcaattttct gctcgaatta agtgacaatg gggccggccg
      901 catgctcaca ttgtctgcca gtgtgctcgc catcatcgcg ctgtgctcgc caacagaaga
      961 ttctgctcac attggctgcc aatgagcaaa actgccggaa aaatcaggca actacccgtt
     1021 cgtgctgcct gtagcgctgt cgtctttcgc cccgctttct cgaaagtcgc cggctgcttg
     1081 gtgtttcgga acacggcaat caagaacagt tttgagaggg caagccgggc caaaagcgcg
     1141 gtttggccag ggcttggccg ggatcgtcag aaaccatggt taacgagaac ggctggcgct
     1201 cgtacggccg aacctcgttg gcagggattg tgagcacgtt cctccgttgg cgagcacgac
     1261 gcgatgatgg cgagcacggt ggcagctaat gtgagcacga gcgccgcccc attagcagat
     1321 aatccgagca ggaacccgct ccgtttgcga gcacatgcgt cgatgattgc gagcggctac
     1381 agctagacaa ggggtacccc ggcagcagcg ggtcgtgcgc ggaacgccgg cccgcggcga
     1441 ccggccggcg ctcgatgcgg gggcgcatcc gatcacgcgc cgcgggtgac atctctatgg
     1501 gggttcacgt ccccgatgtg ccaaaagtga cggtaaggaa tctgtgacat atcaatggtt
     1561 tacatcggca tgtctggtat aacgcattga aatcatgaag ttttaagctt ttcctgcggc
     1621 gttctgcgac tcggtgctgc ctgcgtaacc tgctggggac cccgggaggc ggccatgcag
     1681 cggtggcgag agcagtatct cggactggcg cagtttccgc cggccatggc cgctgccgag
     1741 gttgaccagt tcttcaccct gaacgcgagc gatttgaccg ctgtcgcaac gcggcgaggg
     1801 ccgctccatc ggctgggtgt cgggctgcag atcggtttcc tgcggatgac gggccgcacg
     1861 ctgaactcgt tccagatggt tccggtcgcg gtgctggagc atctgggagc acagcttggc
     1921 ctgacggtgc cacgcctcgc gtccatccgg gcattgtatc ggcggcggcg cacgctgttc
     1981 gagcatcaac gcgtcgccat ggcagcgctg ggcttccgcc acctgaccga tcacgccgcg
     2041 cgcggtctga cggcgcattt gcgtcgctcc gccgaagcga cattcagtgg cgcggctctg
     2101 accaggctcg tccgggtctg gctccacgag catcgctaca tgctgcctgg cgacaagcgc
     2161 g
//
LOCUS       DATGEM010000279         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1649412, whole genome shotgun sequence.
ACCESSION   DATGEM010000279 DATGEM010000000
VERSION     DATGEM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649412"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VKQ27_08680"
     CDS             complement(<1..284)
                     /locus_tag="VKQ27_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89046.1"
                     /translation="MWENRCKIAVSGVGYSKVSRSADVPLAAHALTAVKEAVADSGLR
                     MEDIDGLATYPELPATGHAEVDGISVVSVNCMMAMLKLPNLTWHIQVGTT"
     gene            complement(296..721)
                     /locus_tag="VKQ27_08685"
     CDS             complement(296..721)
                     /locus_tag="VKQ27_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY89047.1"
                     /translation="MSDWNNVTRPIPVPDQYTRPFWDAAKRNALELQRCQSCGHFQHP
                     PYATCVQCVSTDLKFEPVRGQGAIYAYTIMYHTGDKRFASAVPYASIIVELDDAPGAL
                     LAGNLLGSEYTEAKVGRRVEVVFEQLNDDITLPQFRLAR"
     gene            complement(714..1307)
                     /locus_tag="VKQ27_08690"
     CDS             complement(714..1307)
                     /locus_tag="VKQ27_08690"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HLY89048.1"
                     /translation="MSQARRLPMTFEQFLEWERRQERKHEFIDGEVIARAGASEAHNI
                     IQVNLLTASVPKLRGSACRPFPSDMLVRTGTQRGRYPDMTIDCGPRRSDNLIAPDPRV
                     VFEILSQDSQHPDRTIKLADYNATRSTAHYVLIEQKAPLVDVYSRGPHGDFSLRPQEI
                     RGLEGIVELPAIGISLTMAEIYGGLDDLDPIQDEANE"
     gene            1389..>2264
                     /locus_tag="VKQ27_08695"
     CDS             1389..>2264
                     /locus_tag="VKQ27_08695"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89049.1"
                     /translation="MDAIAEDTRLPVDIDAHPLVQAALTLRPELRRYHKDIEREQRFP
                     PALVVQLHEAGVYRMLLPRALGGFQVDPLTYTRVVEVLAEGAGSVGWNIANNSIGQLV
                     TLGLPDEGVAEIYPSGHATVMAGTAVQGGGKAVAVEGGYRVTGHWTFGSGCQEATWML
                     GSFQILDDNGEPRRRADGGMYWRGVFRRDEAVVLPGSWDVAGLRGTGSFDWTVTDVFL
                     PERRSVAHVGVPVDNQWSRWPGVTYALPSVAWVGPHHSSVITGIARAGIDALIALAVE
                     KTPRGRSGMLCEYPQV"
BASE COUNT          359 a          635 c          835 g          435 t
ORIGIN      
        1 ttggtggtgc cgacctggat gtgccaggtg aggttgggca gcttcagcat cgccatcatg
       61 cagttgacgg agacgacgga gatgccgtcg acctcggcgt ggccggtggc gggcagttcc
      121 ggataggtgg cgaggccgtc gatgtcctcc atgcggaggc cggagtcagc gaccgcttcc
      181 ttcaccgcgg tgagtgcgtg cgcggcgagc gggacatcgg cgctgcgcga gactttcgag
      241 tagcccacgc cgctgacggc gatcttgcat cggttctccc acatgtcggt ctccttcagc
      301 gggccaggcg gaattgcggg agggtgatgt cgtcgttcag ctgctcgaac acgacttcga
      361 cgcggcggcc taccttggct tcggtgtatt cggagccgag caggttgccg gcgagcaaag
      421 cgcccggcgc atcgtcgagt tcgacgatga tgctggcgta gggcacggcg gatgcgaagc
      481 gcttgtcgcc ggtgtgatac atgatcgtat aggcgtagat ggcaccctgg ccacgcacag
      541 gctcgaactt gaggtcggtg gagacgcact gcacgcaggt ggcgtagggc gggtgctgga
      601 agtgcccgca gctctggcag cgctggagtt ccagcgcgtt gcgtttggcc gcgtcccaga
      661 acggcctggt gtattggtcg ggcaccggga tggggcgggt cacgttgttc cagtcactca
      721 ttggcttcgt cctgtatcgg gtcgaggtcg tcgagaccgc cgtagatctc ggccatggtc
      781 agcgagatac cgatggcggg gagttcgacg ataccttcca ggcccctgat ctcctgtggg
      841 cgcaaactga agtcgccatg cgggccacgg ctgtagacat cgacgagcgg tgccttctgc
      901 tcgatcaata cgtagtgtgc ggtcgaacgc gtggcgttgt agtcggcgag cttgatggtc
      961 cggtctggat gctgcgagtc ctgggatagt atctcgaaca cgacacgtgg gtcgggtgct
     1021 atcaggttgt cgctgcgtcg tggtccgcaa tcgatggtca tgtcgggata gcgccctcgc
     1081 tgggtgccgg tgcgtaccag catatccgag gggaatgggc ggcatgctga tccgcgcagc
     1141 tttggcaccg aggccgtgag caggttcacc tggatgatgt tgtgcgcctc gctcgcgccg
     1201 gccctggcga tcacctcgcc atcgatgaac tcgtgcttgc gctcctggcg ccgttcccat
     1261 tcgaggaatt gctcgaaggt catcggcagc cgcctggcct gggacatcac ctttctccgt
     1321 gccacaggta ctgtagcatg ggcagggtgg cgtcaatcgt gaacgaggca cccccggagg
     1381 aaaacgcaat ggacgcgatc gccgaagaca cgcgcttacc ggtcgatatc gatgcgcacc
     1441 cgctggtgca ggcggcgcta acgctgcggc cggagttgcg ccggtatcat aaagacatcg
     1501 agcgcgagca gcgcttcccg ccggctctgg tggtgcaact gcacgaggcc ggcgtctacc
     1561 gcatgcttct gccgcgcgcg ctcggcggtt tccaggttga tccgctgacc tatacgcggg
     1621 tggtggaggt gcttgccgaa ggggctggtt cggtggggtg gaacattgcc aacaacagca
     1681 taggccagct tgtcacactc ggactgccgg acgagggggt ggccgaaatc tatccgagtg
     1741 gccatgccac tgtgatggcg ggcaccgcgg tgcagggcgg cggtaaggcg gtggctgtcg
     1801 agggtggata ccgcgtcacc gggcattgga cctttggcag cggatgtcag gaagcgacct
     1861 ggatgctggg gagtttccag atcctcgatg ataacggcga gcctcggcgc cgggcagacg
     1921 gcggcatgta ctggcgcggc gtgttccggc gggacgaagc agtggtgctg ccgggaagct
     1981 gggacgtggc agggctgcgc ggcacgggca gcttcgactg gacggtgacg gatgtgttcc
     2041 tgccggagcg gcggagcgtg gcgcatgtcg gtgtgccggt ggacaatcag tggtcgcgct
     2101 ggccgggtgt gacttacgcg ttgcccagcg tcgcgtgggt ggggccgcat cacagctccg
     2161 ttatcacggg catcgcgcgc gccgggatcg atgcgctgat cgcgctagcg gtggagaaga
     2221 caccacgcgg gcgcagcggg atgctgtgcg agtatccaca ggtg
//
LOCUS       DATGEM010000280         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_687396, whole genome shotgun sequence.
ACCESSION   DATGEM010000280 DATGEM010000000
VERSION     DATGEM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687396"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1092
                     /locus_tag="VKQ27_08700"
     CDS             187..1092
                     /locus_tag="VKQ27_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HLY89050.1"
                     /translation="MQDLDETTVTEAALAQMANTPDPRLKEIMDAAVRHLHAFARDVR
                     LTPQEWLTGIQFLTAVGQACTPFRQEFILLADVLGVSALVNAMHDKKARELGTQSSLL
                     GPFYREGAPELPLGSSIVANPSIAEIVVYGRVTDNDGKPVPNALVQVWQTSEHGLYDL
                     QAENAAEMMDLRANFRTDAKGNYHFRTVRPLGYSIPMDGPVGEMVTAQNRHGFRPSHI
                     HFLIGGDGYRELVTALYFGDDVHIDSDTVFGVSGSLVVGAKDDTNSPIPGMKAVRYDF
                     RLAHAEEGDTGRVGADPSKLMKAAE"
     gene            1136..1924
                     /locus_tag="VKQ27_08705"
     CDS             1136..1924
                     /locus_tag="VKQ27_08705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLY89051.1"
                     /translation="MARRLIDISSPLKAGIKSDPPHMLPQIEYLDHHMTAPAMAEYMG
                     IRVDQLPDGEYAAVERVAISTHNGTHLDAPYHYFSTMNHALKPGGEPSWRIDEVPLEW
                     CFQPAVKLDFRHLPDGYVVTPNDVDAELGRIGHTLKALEIVVVNTAAGAKYGQDDYVD
                     SGCGMGKAATLWMLERGVRLVGTDAWSWDAPFSHTRRKVAETGDPGLVWEGHRAGREI
                     GYSHLEKLHNLEGLPADGFQVVCFPVKVHRASAGWTRAVAIIDD"
     gene            1952..>2622
                     /locus_tag="VKQ27_08710"
     CDS             1952..>2622
                     /locus_tag="VKQ27_08710"
                     /inference="COORDINATES: protein motif:HMM:NF042722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6635 family protein"
                     /protein_id="HLY89052.1"
                     /translation="MTPELARSAVESAVRRYCADRRARIPAFVDRHFSLRGAAALHQA
                     ALGWDILRTPFNLAMAAPAAGLHLAAVSARRLGGKRVARALSSRRLLLPTAVARRMEW
                     LINTELLELPCHIGDRISTRDALAEAIMAEPAVRGALEEPLAAIGRRGSEPAFRAQIE
                     RMLAEYAVTRGAAAEIATALTSLGTGALTLGKLTPGFVSFGPALASMAAQQAAISSFP
                     LGAGLG"
BASE COUNT          465 a          874 c          855 g          428 t
ORIGIN      
        1 gtccgcgccc atgtccaccc ccatgtcatt gcgaggagcg gagcgacgaa gcaatcccgg
       61 ggtaagctga cagcactgcg ccggccctat cgccggccgc cgctacgccg cccaccataa
      121 cagtcgagcc tttgtctgcc cctcgcccgc cgctatgctg cgggccttga gccacaggag
      181 cgtgccatgc aggacctgga cgaaaccacc gtcacggaag ccgccctcgc gcagatggcg
      241 aacacgccag atccgcgcct caaggaaatc atggatgcgg cggtgcgcca cctccacgcc
      301 ttcgcccgcg atgttcgcct gacaccgcag gaatggctca ccggcatcca gtttctcact
      361 gccgtaggcc aggcctgcac gccgttccgc caggagttca tcctgctcgc cgacgtgctc
      421 ggtgtcagcg cactggtcaa cgcgatgcac gacaagaagg cgcgtgaact cggcacgcaa
      481 agcagcctgc tcggtccgtt ctatcgcgag ggcgcgccgg agctcccgct cggctccagc
      541 atcgtcgcga atccctcaat cgccgagatc gtcgtctacg gccgcgtcac cgacaacgac
      601 ggcaagccgg tccctaacgc actggtgcag gtgtggcaga ccagcgaaca cggcctctat
      661 gacctgcagg ccgagaacgc cgccgagatg atggacctgc gcgccaattt ccgcaccgac
      721 gcgaagggca attaccattt ccgcacggtg cgtccgctcg gctactcgat cccgatggat
      781 ggaccagtgg gtgaaatggt gacggcgcag aaccgccacg gcttccgccc ctcgcacatc
      841 catttcctga tcggcggcga tggctaccgc gaactggtca ccgcgctgta tttcggcgac
      901 gacgtgcaca tcgattccga cacggtgttc ggcgtatcgg gttctctggt ggtcggagcg
      961 aaggacgaca caaactcgcc gatcccggga atgaaggcag tgcgatacga cttccgtctg
     1021 gcacatgccg aggaaggcga taccggtcgc gtcggcgccg atccctcaaa gctgatgaag
     1081 gccgctgaat aatggccggg tcacgcccgg ccgtcatgaa tggaggttgt gcacaatggc
     1141 tcgccggctg atcgacatct cctcgccgct gaaggcgggg atcaagagcg atccgccgca
     1201 catgctgccg cagatcgagt acctggatca ccacatgacg gcgcccgcga tggccgaata
     1261 catggggatc cgtgtcgatc agttgcccga tggcgaatac gcggcagtgg agcgcgtcgc
     1321 catcagcacg cacaacggca cgcatctcga cgccccgtac cactactttt ccacgatgaa
     1381 ccacgcgctg aagccgggtg gcgaaccgtc gtggcgcatc gacgaagtcc cgctcgaatg
     1441 gtgcttccag cctgcggtga agctggactt ccggcacctc ccggacggct acgtggtgac
     1501 gccaaacgat gtggacgcgg agctcgggcg tatcggccac acgctgaagg cgttggagat
     1561 cgtcgtggtc aacacggcgg ccggcgcgaa gtacggccag gacgactatg tcgattcagg
     1621 ctgcggcatg ggcaaggccg cgacgctctg gatgctggag cgcggcgtgc ggcttgtcgg
     1681 caccgacgcc tggagctggg acgcgccatt ttcccacacg cgacgcaagg ttgcggaaac
     1741 cggcgatccc ggcctggtct gggagggcca tcgcgccggc cgcgagatcg gctactcgca
     1801 cctggagaag ctgcataacc tggaagggct gcccgccgac ggcttccagg tcgtttgctt
     1861 ccccgtgaag gtgcatcgcg cctcggcagg ctggacgcga gcggtggcga ttatcgacga
     1921 ctaagccgcg cagccccatt tgcctggggt gatgacgccg gagttggcac gcagcgcggt
     1981 agaatccgcg gtccgccgct attgcgcgga caggcgcgcg cgcatcccgg cgtttgtcga
     2041 ccgacatttc tcactgcgtg gtgccgcggc gctgcatcag gccgccctcg gctgggacat
     2101 tctacgcacc ccgttcaacc tggcgatggc ggcaccagcc gcagggctgc accttgccgc
     2161 agttagcgcg cggcggctcg gcggcaagcg ggtcgcgcgg gcactttcga gccgtcggct
     2221 gttattgccg accgcggtcg cacgccgcat ggaatggctc atcaacaccg agctgctgga
     2281 actgccctgt cacatcggcg atcgcatttc gacgcgcgat gcactcgccg aggcaattat
     2341 ggctgaaccc gcggtgcgcg gcgcactcga agaaccgctg gcggcgatcg gtcgaagggg
     2401 cagcgagccg gcattccgag cacagatcga acgcatgctg gcggaatatg ccgtgacgcg
     2461 cggtgccgca gcggagatcg ccacggcttt gacatcgctg gggacagggg cgctgacgct
     2521 gggcaagctg acgcctggct ttgtctcatt cgggccggca ctggcgtcga tggccgctca
     2581 gcaggcggcg atctcatcgt tcccgctggg cgccgggctc gg
//
LOCUS       DATGEM010000281        13942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1382160, whole genome shotgun sequence.
ACCESSION   DATGEM010000281 DATGEM010000000
VERSION     DATGEM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382160"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VKQ27_08715"
     CDS             complement(<1..901)
                     /locus_tag="VKQ27_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLY89053.1"
                     /translation="MSELPKTFDPAAIEARWYSHWEETGQFRPERPDAEPFTIVIPPP
                     NVTGSLHIGHALDNTLQDILVRHARLKGKDALWVVGTDHAGIATQMVVERQMNAKGEK
                     RTDFARDAFIDKVWEWKAESGGTITRQLRRLGASCDWANERFTMDEGFSKAVVHVFVK
                     LYEQGLLYRDKRLVNWDPGLKTAISDLEVETREIAGKFWHLRYPLADGSGFIAVATTR
                     PETMLADMAVAVNPEDERYKALIGKQVRLPITGRLIPIVADEHADPGLGSGAVKITPG
                     HDFNDFEVGKRAGIAAGNMLNMLD"
     gene            complement(958..1521)
                     /locus_tag="VKQ27_08720"
     CDS             complement(958..1521)
                     /locus_tag="VKQ27_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HLY89054.1"
                     /translation="MGQINQEPSMEDILASIKRIIAEDSEAVGARAPRLRREAMAAVA
                     SSPLVASAGDEQPPQSDDEAAPEEAPAPTEVETPVKPDPSNDVLELTSPMPEAATTDA
                     PESETAAALVGLNGLVSSSAAQASRNAFAALSQLQVRAETGQENTLEGLVADLLRPML
                     REWLDRELPTLVERMVSAEITRLSGRG"
     gene            complement(1538..2971)
                     /locus_tag="VKQ27_08725"
     CDS             complement(1538..2971)
                     /locus_tag="VKQ27_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HLY89055.1"
                     /translation="MGALVVGLNGGTSAGATTLTDALVATYGGNPTLTAQRAQLRATD
                     EQAAIVRAAGRPTLGGSASYTQGLNGLRELQGYNRSLTAGAQLSVPLFQGGRVHAATR
                     AADSRVASGRQTLRATEGDVFVQAVSAYLDVLRDRAIVELNLNNVKVLQTTLEATTDR
                     FQVGDLTRTDVAQSQARLQDGQAQLIRARSNLVASEENYRRVVGQAAGALDQPPPLPV
                     LPENPDKAEDVAISNNPDIAAAAATVKATHYDVSQARASRMPTVSASAGTQYYQYMGF
                     AGNGLGVPVGNVRGDASQAGVTLNVPLYQGGLPGAQVRQAQAFESAALENETAIERQV
                     VANARAAFSSYQAAVEVIRSSQEALKANTLALEGVRAEQGAGLRQVLDTLNAEQEKLS
                     SEVQLVSAQHDAYVAGFALLNAMGLVDYKHLGLEGGALYDPTMNYRHAIHARGDWSES
                     PTPKPVAKPTYGPQIRPENPPVTSPAN"
     gene            complement(3028..3681)
                     /locus_tag="VKQ27_08730"
     CDS             complement(3028..3681)
                     /locus_tag="VKQ27_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLY89056.1"
                     /translation="MSEQDFDQLRRAMVTNQLRTNKVTDAAVLTAMGTTPRERFVAEG
                     QGALAYRDTIVPLGEGRGLNPPIATGRLLDAAAPQPGEHVLVVGAGTGYVAALLAAMG
                     CIVTALEESQDLAARAKTALQDLAGITLAQGPLAAGWPAKAPYALVYVDGAVEHLPEA
                     IIGQVAEGGRLVGAIAERSVIRLILGRRAEGAFAYNAFADVETASLPGFAPAKVFTF"
     gene            3848..3921
                     /locus_tag="VKQ27_08735"
     tRNA            3848..3921
                     /locus_tag="VKQ27_08735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3880..3882,aa:Cys,seq:gca)
     gene            complement(3986..5125)
                     /locus_tag="VKQ27_08740"
     CDS             complement(3986..5125)
                     /locus_tag="VKQ27_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY89057.1"
                     /translation="MTRRRKGRARFLPKYVTSFEDRHGKARYRFRRKGYEDHYFKAEL
                     GSDEFRQEYAACMKAVVVSEEQQTRWAIGTIGDLVARYIAVPARLAPTEATQGKIRSI
                     LEKWRDEYGTAYVADVNFQHIDDIITEKRKKVLVGKRWEGGIEAARKLRKELVRLFDH
                     AEKIGMIPKGANPVKHAEKVRVAAGQKSKGYYSWTEQDIDQYRARHALGSKPRLALEL
                     LLWTGQRRGDGYLFGPNDIKNGRFEITQGKTGKEMSLTIAPQLLAAIQAMPPLPADSP
                     AFLLSELGRPFSYASFGNKMRDWCDQAGLPQCTAHGLRKANARRLAERRMGNSSIKAV
                     GGWTNDREVSIYTAAANQAHLADLAITDLSDWETQKGRENETRDV"
     gene            complement(5122..5307)
                     /locus_tag="VKQ27_08745"
     CDS             complement(5122..5307)
                     /locus_tag="VKQ27_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89058.1"
                     /translation="MSARNVSFREADLKRALAAAQKSGIRQYRVEIAVDGTIAVVVGA
                     DAVKQARKNSMDELLGR"
     gene            complement(5304..5603)
                     /locus_tag="VKQ27_08750"
     CDS             complement(5304..5603)
                     /locus_tag="VKQ27_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89059.1"
                     /translation="MTSADEIARGLSKAQREKIIRSDVIVLRNRWDTRIAINLAKIGV
                     CERGRHINQFELTPLGLEVREILRSPRADTPGLAREIRAADYALLPALPSEEAKG"
     gene            complement(5590..6048)
                     /locus_tag="VKQ27_08755"
     CDS             complement(5590..6048)
                     /locus_tag="VKQ27_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89060.1"
                     /translation="MRYYLDTEFNGFGGQLLSLALVREDGESMYWVSSHNCDQHWDGW
                     VLQNVVPLYHECPVEADMATPLPQQIMNLMDGDGEPVIVTDWPDDIRYFCQAIITGPG
                     QMIPLPDGLEFELRRVDAYPTTLPGAVQHNAWWDAMALRHMLIGAPHDQR"
     gene            complement(6045..6827)
                     /locus_tag="VKQ27_08760"
     CDS             complement(6045..6827)
                     /locus_tag="VKQ27_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89061.1"
                     /translation="MHHELRKAVTAEVRRHIAEGDCPTKRSYNIETLENVIDDLTRTE
                     AAGGSPKRGDKIKWVRALDRRPLAVSGLEYVTVASWGLPGIPLVVTLQEVAGSFSMGW
                     FDRTEATTGGERLRDALDGFVDALTSGRNNPPTVLSINHDVLRACLIDAEAALAQPVR
                     VDDHALATSEVVDRGTTIDDQQDETAFAQPVQDAGVPWGQVDPVTRNNAAEINEARRL
                     LKIAGDEMIAIEPNPSVWVSENSALRAIVHLGRRAFGRERGE"
     gene            complement(6827..7066)
                     /locus_tag="VKQ27_08765"
     CDS             complement(6827..7066)
                     /locus_tag="VKQ27_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89062.1"
                     /translation="MDNREAIARIIDRFSCTHWDAGAKYDLTAEILALLTHTEKTDEQ
                     EIARPRCGCPTETVRRVIQDHGTCGMGGCPYGGDI"
     gene            complement(7059..7403)
                     /locus_tag="VKQ27_08770"
     CDS             complement(7059..7403)
                     /locus_tag="VKQ27_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89063.1"
                     /translation="MADGDDLRGNTGLPIFPERMPLWHRILCVLGYHKPEIRINNRPD
                     QCWGDRSRMRDACGRCGTYMNTQISDKAREAAAMEYAIRTYPMPAPRKAEFQAYVDGL
                     RPQRAPKEPSHG"
     gene            complement(7396..7947)
                     /locus_tag="VKQ27_08775"
     CDS             complement(7396..7947)
                     /locus_tag="VKQ27_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89064.1"
                     /translation="MTDTQPQSSEYARGSDEEDDHKTLIERLTGRARYLRDMNRVKSP
                     QLMEEAAEGLADQRRVMETVCVNLMRAPENAAAIAAYLSAKLRGASDADAVKEGEAAL
                     SPTPAQPQEGVAPDGWTFGGSEPPVGAWVTAPDGQTISQYGIRETQSQARRVGLVGVC
                     AKLEMFSELIACLAAAPKEANHG"
     gene            complement(7944..8153)
                     /locus_tag="VKQ27_08780"
     CDS             complement(7944..8153)
                     /locus_tag="VKQ27_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89065.1"
                     /translation="MSAQLGLEGVIQGLIGDLDALRSGSISVNDAIARSMLAKQIFNG
                     VRIYLNGSKMLASNARQINGSETAQ"
     gene            complement(8170..8427)
                     /locus_tag="VKQ27_08785"
     CDS             complement(8170..8427)
                     /locus_tag="VKQ27_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89066.1"
                     /translation="MSDSKAGFTCVICQRFICTRWNMPGRVSQIEPVCTGCEGQYTQG
                     IGKPTDGSFRDRRNVIRGFSLAEALHDEAARQNWSKRHAIA"
     gene            complement(8420..8995)
                     /locus_tag="VKQ27_08790"
     CDS             complement(8420..8995)
                     /locus_tag="VKQ27_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HLY89067.1"
                     /translation="MSELLDLLGDPVMRRSAEFAGCERILLSRAWGPGPRAFVIGCNP
                     STANALQDDPTSLWWNAWFQLFGFGRYDAGNLYPFCSSSPAECRKRVEAIDAGLDWSD
                     RDQLHFVNLPRVVEMAKAADQVFVCWGNIAWDQEWIEHVVEEIQTGEPPYPDLWCWGT
                     TKSGAPTHPMARGKHRIPRDQQPIIWRKADV"
     gene            complement(9093..9437)
                     /locus_tag="VKQ27_08795"
     CDS             complement(9093..9437)
                     /locus_tag="VKQ27_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89068.1"
                     /translation="MSAPFFDAVRLTKPLFVPGIRLVASDVMTVLFEDWSGVRSPSRA
                     RRRRARGHRQNIRFRQIADPKMIQGPDGTLYGHPHTIARVERELAAEMKRRSQSMREH
                     AVTSALMGRNPW"
     gene            complement(9434..9916)
                     /locus_tag="VKQ27_08800"
     CDS             complement(9434..9916)
                     /locus_tag="VKQ27_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89069.1"
                     /translation="MKRQHHPMSWRRRPSGVPGDPELRSLEHYKRNPDRVPAGRMVRI
                     WSAEWSAFWRPNGCGYTTQLAEAGRFLIQDAFAKTRGCDPDKRIWFAFIAPGDTGALA
                     RAERHWYEDLGDVLWWKFPVTEAPWVGTPLDSSWPGYHTHWTPLPPMPHKPKRGGQWA
                     "
     gene            complement(9913..10221)
                     /locus_tag="VKQ27_08805"
     CDS             complement(9913..10221)
                     /locus_tag="VKQ27_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89070.1"
                     /translation="MSEQFYDDVVAPALLDLAKQCRERGMPFLATVEYAPGEYGTTAD
                     LPPAAERSLPMDWSYVAARSQGNADTLILHLMKQAAERGHGSVTLAQLGIPHSPARAA
                     "
     gene            complement(10218..10955)
                     /locus_tag="VKQ27_08810"
     CDS             complement(10218..10955)
                     /locus_tag="VKQ27_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89071.1"
                     /translation="MVRALLAGRKTQTRRLLSRARVFATPERPAFTLAAEHMARALQG
                     ASGFRHLDGTGWFWEADAFEWQAPAERTGWMANIGYAAGDRLYVRESYYQQGHWEQAA
                     GVVTKGGRPKWKFVPDDDAIRFDPPIAFRKARHSADPGTTAWHRRLARFMPRSASRLT
                     LIVEDVRVEQLQLISEADAQAEGVDRLVWDGDGGWYASDAGTHYCGFAGIWQHLHGAE
                     SWDANPFVVPVTFRVERGNIDQIGRTA"
     gene            10987..11259
                     /locus_tag="VKQ27_08815"
     CDS             10987..11259
                     /locus_tag="VKQ27_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HLY89072.1"
                     /translation="MAFTIDPAEAVYDFRSSTVVFYLEDGGARVRCGVCQLALVDDYG
                     ATSTNQVEMLEVFRENCVAIGKAAERKHRGGLTEPNGHILVRTGDR"
     gene            complement(11268..11879)
                     /locus_tag="VKQ27_08820"
     CDS             complement(11268..11879)
                     /locus_tag="VKQ27_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89073.1"
                     /translation="MTDRFIPSLDDEALQAMCANSYRDFHAKGLDYLCLHRSEALTLK
                     AYFFAGECTESPELVVPHDHRYGFTTHVLAGGLVERRWAECDAGQGTVYQQFDYSTPL
                     NGGDGFTWKREVRLGGWDRGVHGAGTAYMTRSDQIHTLANVQRGTAVVIQQFRDVVLG
                     EPTRAFRRGLGHEAPSTDGLYSRMTPDQALQRIAQLHALGWPA"
     gene            complement(12045..12539)
                     /locus_tag="VKQ27_08825"
     CDS             complement(12045..12539)
                     /locus_tag="VKQ27_08825"
                     /inference="COORDINATES: protein motif:HMM:NF045163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89074.1"
                     /translation="MKLLIMSCCATKRPNPAPMPAIDRYDGPMWQTLRARLAELPAAV
                     AAMRSGELVVRVLSAQHGFIPVDIAILPYEQRLTSRRADDLLSMCTSDHAAIADVFAE
                     AEAVMFAGGALYRDTMWRASRGGLAQIMKVSETDGAGIGEHRAQLGDWLGVHFPCSER
                     GLAA"
     gene            complement(12536..13483)
                     /locus_tag="VKQ27_08830"
     CDS             complement(12536..13483)
                     /locus_tag="VKQ27_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HLY89075.1"
                     /translation="MGADTKIEWTDATVNFWWGCTKVGPGCDHCYAEVWSRRTGGGIW
                     GVGVPRRKIKSAVKLIHRLDNDYSWWAADHYIGQMHATSRRRVFIQSMSDLFDIEVPV
                     EWFAEAWQHIVSCNRLAIQIVTKRVSVVEKRLAEIGASWPAHAGLLISVVNQAEADRD
                     VPRLIALKAKLGIPWVGLSMEPLLGPVVLRKEWLEHLDWIIVGGESGAAARPINPDWV
                     RALRDQCAAALVAFLFKQWGEWAPMSAARIARDGPVTDRWGNVADWMSRYVVFDDDAG
                     AARVRGHSFTEHATDLVYRVGKKSAGRLLDGVQHDGVPA"
BASE COUNT         2363 a         4594 c         4423 g         2562 t
ORIGIN      
        1 catcgagcat gttgagcatg ttgccagccg cgatgccggc gcgcttgccc acctcgaaat
       61 cgttgaagtc gtggcccggc gtgatcttca ccgcgccgga acccagtccc ggatcggcat
      121 gctcgtcggc gacgatcgga atcaggcggc cggtgatcgg caggcggacc tgcttgccga
      181 tgagggcctt gtagcgctcg tcctcgggat tcaccgccac cgccatgtcg gcgagcatcg
      241 tctcggggcg cgttgtcgca accgcgatga agcccgagcc atctgccagc ggatagcgca
      301 agtgccagaa cttgccggcg atctcccgcg tctcgacctc gagatcggag atcgcggtct
      361 tgaggccggg atcccagttc accagtcgct tgtcgcggta cagcaggccc tgctcgtaga
      421 gcttgacgaa gacgtggacg acggccttcg agaagccctc gtccatcgtg aaacgctcat
      481 tcgcccagtc gcacgaggcg ccgaggcggc ggagctggcg cgtgatcgtg ccgccgctct
      541 cggccttcca ctcccacacc ttgtcgatga aggcgtcgcg cgcaaaatca gtgcgcttct
      601 cgccctttgc gttcatctgc cgctcgacga ccatctgcgt cgcgatgccg gcatgatcgg
      661 tgccgaccac ccacagcgcg tccttgccct tcagccgcgc gtggcggacg aggatgtcct
      721 gcagcgtgtt gtccagcgca tggccgatat gcagcgatcc cgtcacgttg ggcggcggga
      781 tcacgatggt gaagggctcg gcgtcgggcc gctcgggccg gaactggccg gtttcttccc
      841 aatgggaata ccagcgggcc tcgatggcag cgggatcgaa ggtcttgggg agttcgctca
      901 tgacggcgtc cgcctagcag gcgccgatag caaatgccaa gcgccgcagt cggggtttca
      961 gccgcgcccg ctcagacgcg tgatctccgc cgaaaccatc cgctccacga gggtgggcag
     1021 ctcgcggtca agccattcac gcagcattgg gcggagcaga tcggcgacca gaccctccag
     1081 cgtgttctcc tggccggttt cggcgcgaac ctgcagctgc gagagcgctg cgaaggcgtt
     1141 gcggctcgcc tgtgcggcag acgacgacac caggccgttc aggccgacca gcgcggcagc
     1201 ggtttcgctc tcgggagcgt cggtggtggc ggcttccggc atcgggctgg tgagctccag
     1261 aacatcgttg gaggggtcgg gtttgacggg cgtttcgact tccgtcggcg ccggagcctc
     1321 ttcgggagct gcctcgtcat ccgactgggg cggctgctcg tcccctgccg aggcgaccag
     1381 cgggctcgac gcaaccgcgg ccatcgcctc ccgacgcagg cgcggagcac gcgcgccaac
     1441 cgcctcgctg tcctcggcga tgatgcgctt gatggaggcc agaatgtcct ccatcgacgg
     1501 ttcttggttg atctgcccca taagccgcaa tttatcccta gtttgcgggc gaagtcacgg
     1561 ggggattttc aggccggatc tgcggcccgt aggtcggctt ggcgacgggt ttgggcgtgg
     1621 gactttccga ccagtcgccg cgtgcatgga tggcgtgccg gtagttcatg gtgggatcgt
     1681 acaacgcccc gccttccagc ccgagatgct tgtaatccac cagccccatc gcgttcagca
     1741 gcgcgaaacc ggcgacatag gcgtcatgct gcgcggagac gagctgcacc tcgctgctca
     1801 gcttctcctg ttcggcgttc agcgtgtcca gcacctgccg caggcccgcg ccctgttcgg
     1861 cgcggacgcc ttccagcgcc agcgtgttcg ccttgagcgc ctcctggctc gatcgaatca
     1921 cctcgaccgc cgcctgatag gacgagaagg ccgcgcgagc attggccacg acctgccgct
     1981 cgatggcggt ctcgttctcg agcgcggccg attcgaacgc ctgcgcctgc cgtacctgcg
     2041 ccccggggag gccgccctgg tagagcggaa cattcagggt cacgcccgcc tgagaggcgt
     2101 cgccccggac attgccgacc ggcacgccca ggccgttccc ggcaaagccc atatactgat
     2161 aatattgggt gccggcggac gcggagacag tcggcatccg gctggcacgc gcctgcgaca
     2221 cgtcataatg cgtggccttc accgtggcgg cggcggcggc gatatccgga ttgttggaaa
     2281 tcgcgacatc ctcagccttg tcggggttct ccggcagcac gggaagcggc ggcggctggt
     2341 cgagcgctcc ggccgcctgc ccgaccacgc ggcgataatt ctcttcgctc gcgacgaggt
     2401 tgctgcgcgc gcggatgagc tgcgcctggc cgtcctgaag gcgcgcctgc gactgggcga
     2461 cgtcggtacg ggtgaggtcg ccgacctgga aacgatccgt cgtcgcctcc aacgtggtct
     2521 gcaacacctt cacattgttg agattgagct cgacgatcgc gcgatcgcgc agcacgtcca
     2581 gataggctga cacggcctgg acgaacacgt caccctcggt cgcgcgcagc gtctggcgtc
     2641 ccgaagccac ccggctatcg gcggcccgcg tcgcggcatg cacgcggccg ccctggaaga
     2701 gcgggacgct caactgggca ccggcggtga gcgagcgatt atagccctgc aactcgcgca
     2761 gaccgttcag gccctgggtg tagctggcgc tgccccccag cgtcgggcga ccggcggcgc
     2821 ggacgatcgc cgcctgctca tccgtggcac gcagctgggc ccgctgagcg gtgagcgtgg
     2881 gattgccgcc gtaggtggcg accagcgcat cggtgagcgt tgtcgctcca gcactggtgc
     2941 ccccgttcag tccgaccacg agcgcgccca acgcagcgcc cgccttcacc acactccacc
     3001 ccttcatgct ccccccttgt tatccgtcta gaatgtaaag acctttgctg gcgcgaagcc
     3061 cggcagcgaa gccgtttcga cgtcggcgaa ggcgttgtag gcaaaggcgc cctcagcccg
     3121 acgcccgagg atcagccgga tgacgctgcg ctcggcgatc gcgccgacaa ggcggccgcc
     3181 ttcggcaacc tgaccgataa tagcctcggg cagatgctcg accgcgccat cgacatagac
     3241 gagcgcgtag ggcgccttgg ccggccagcc ggccgcgagc ggtccctgcg ccagcgtgat
     3301 gccggccagg tcttgcagcg ccgtcttcgc gcgtgcggcc agatcctggg attcttcgag
     3361 cgcggtgacg atgcagccca tcgcagcgag cagcgcagcg acatagcccg tgccggcccc
     3421 gaccacgagg acatgttcac ccggctgcgg cgccgcggcg tcgagcaggc gcccggtcgc
     3481 gatcggcggg ttgaggcctc gcccctcgcc cagcggaacg atcgtgtcgc gataggcgag
     3541 cgcgccctgc ccttccgcca cgaaccgctc gcgcggggtc gtgcccatcg ccgtcagcac
     3601 cgccgcgtcg gttaccttgt tggtgcgcag ctggttcgtc accatcgcac gccgcagctg
     3661 atcgaaatcc tgttcgctca tgtttcctga cctcgcacaa ctgtattgtc atagcaatac
     3721 actatctcac gcgagcgatc tctaagcgtt ccccgcccgc ctcgcaacgc cgggtcgccg
     3781 atatggggag attctcgtcg gttgcgtgtt gacaccgcgg ccgccccgtc gcatcagcgc
     3841 cgctctcggc ccgatggcgg agtggttacg cagaggactg caaatccttg cacgccggtt
     3901 cgattccggc tcgggcctcc actctctaaa aatatggcta ttttcccgtc cgttatccgg
     3961 cgttagacgt tttgcgtccg cagggttaga catcgcgcgt ctcgttctcg cgcccttttt
     4021 gcgtctccca atcggacaga tccgtgatcg cgagatccgc aagatgggcc tgatttgcgg
     4081 ccgcagtgta gatcgaaacc tcacggtcat tcgtccatcc accgacggcc ttaatcgagc
     4141 tgttgcccat cctgcgctct gccagtcggc gcgcgttggc cttacgcaga ccgtgcgcgg
     4201 tgcactgcgg cagaccagcc tgatcacacc aatcccgcat cttgttgccg aagctggcat
     4261 agctgaacgg cctgccgagc tcgctgagca ggaacgcggg cgaatcggcc ggtagcgggg
     4321 gcatcgcctg aatagccgcc aacagctgcg gcgcgatcgt caggctcatc tccttgcccg
     4381 tcttgccctg ggtgatttcg aaacgaccat tcttgatgtc gttcggcccg aacagatagc
     4441 cgtcgccccg gcgctgaccg gtccagagca gcagctcaag cgcgagacgc ggcttactgc
     4501 cgagcgcatg gcgggcgcga tattggtcga tgtcctgctc ggtccagctg tagtagcctt
     4561 tggacttctg gccggcggct acgcgcacct tctcggcgtg ctttaccggg ttggcaccct
     4621 tcgggatcat tccgattttc tcggcatggt cgaacaggcg gacgagctcc ttgcgcagtt
     4681 ttcgcgccgc ctcgatgcca ccttcccagc gcttgccgac cagcaccttc ttgcgtttct
     4741 ctgtgataat atcgtcgatg tgctggaagt tgacgtccgc cacataggcg gtgccatact
     4801 catcgcgcca tttttcgagg atggagcgga tcttgccctg cgtggcctcg gttggtgcga
     4861 gacgcgcggg tacggcgatg tagcgggcga caaggtcgcc aatcgtgccg atggcccagc
     4921 gcgtttgctg ctcctcggat acgacgaccg ccttcatgca ggcggcatat tcctgccgga
     4981 actcgtcgct tcccagctca gccttgaaat aatgatcctc gtagcccttt cggcggaacc
     5041 tataccgggc cttgccgtgg cgatcttcga aggacgtgac atatttgggt aggaagcgcg
     5101 ccctaccctt ccggcgcctg gtcatctgcc taacaactca tccatcgaat tcttccgagc
     5161 ttgcttcaca gcgtcggcgc cgacgacgac cgcgatggtg ccgtcgacgg cgatctcgac
     5221 cctgtattgc cggatgccgg acttctgcgc ggcggcgagc gcacgcttca ggtcggcttc
     5281 acgaaaagag acgtttcgcg cgctcaccct ttagcctcct cgctcggtaa tgccggcagg
     5341 agcgcataat cagcggcacg gatttccctc gccagccctg gcgtgtcagc gcgcggggac
     5401 cgcaggatct cccgcacctc gaggccgagc ggggtgagct cgaattgatt gatatgcctc
     5461 ccgcgctcgc agacaccgat tttcgcgagg ttaatcgcta tgcgcgtgtc ccaccggttg
     5521 cgcaggacaa tcacgtcgga acggataatc ttctctcgct gcgccttgct cagcccgcgc
     5581 gcgatctcat cagcgctggt catggggtgc tccgataagc atgtgacgca gcgccatcgc
     5641 gtcccaccaa gcgttatgct ggacagcgcc cggcaacgtg gttgggtagg catcgacgcg
     5701 gcgaagctca aattccaagc catcgggaag cggtatcatc tgacccggcc cagtgatgat
     5761 ggcttggcaa aaatagcgaa tgtcatcggg ccaatcggtg acaatcacgg gctcaccatc
     5821 accatccatg agattcatga tctgctgggg aagtggcgta gccatatctg cctcgaccgg
     5881 gcactcatga tagagtggca cgacgttctg cagcacccag ccgtcccaat gctgatcgca
     5941 gttatgcgac gagacccagt acatgctttc gccatcctcc cgaacaagag cgaggctgag
     6001 aagctgacca ccgaagccat tgaactcggt gtcgaggtaa tatctcattc ccctctctcc
     6061 cggccgaagg cgcggcggcc cagatgaaca atcgcacgga gagcggagtt ctcagacacc
     6121 cacacgctcg ggttgggctc gatcgcgatc atctcatcgc cagcaatctt gagcaggcgc
     6181 cgggcctcgt tgatctccgc tgcattgttc cgcgtgaccg gatcgacctg tccccagggg
     6241 acgcctgcat cctgcaccgg ctgggcgaag gcggtttcat cttgttgatc gtcaatcgtg
     6301 gttccacgat caacaacctc gcttgttgct aatgcgtgat catcaacgcg caccggctgg
     6361 gcgagggcgg cttcagcgtc aatcaggcag gcgcgcagca cgtcgtgatt gattgacagg
     6421 accgttggtg ggttatttcg gccgctggtc aaagcatcga cgaaaccatc aagcgcatcg
     6481 cgcaatcgct cccctcctgt cgttgcctcg gtacggtcga accatcccat cgagaacgat
     6541 cccgctacct cttgcagcgt gacgacaagg ggaatgcccg gaagtcccca tgaggcaacg
     6601 gtgacatatt ccaggccaga gacggcgagc ggtcgccgat caagggcgcg aacccacttg
     6661 atcttgtcgc cacgcttggg tgaaccgccg gccgcctcgg tgcgggtgag gtcgtcaatg
     6721 acgttttcca gcgtctctat gttgtaggat cgcttggtcg ggcaatcgcc ttccgcgatg
     6781 tgccggcgga cttcggcggt caccgctttt cgcagttcgt ggtgcatcag atgtctccgc
     6841 cataggggca tccgcccatt ccgcaggttc catgatcctg gatgacgcgc cgtactgtct
     6901 cggtcgggca tccgcagcga ggacgggcga tttcctgctc gtcggtcttc tcggtgtggg
     6961 tgaggagggc gagaatttcg gccgtcagat cgtacttggc ccccgcgtcc caatgggtgc
     7021 aggagaagcg atcgatgatc cgcgcaattg cttcgcggtt atccatggct tggctccttc
     7081 ggggcgcgtt gggggcgaag accatcgaca taggcctgaa actcagcctt gcgcggcgcg
     7141 ggcatggggt aagtccggat ggcgtattcc atcgcggccg cctcccgcgc cttgtcgctg
     7201 atttgcgtgt tcatatacgt gccgcatcgc ccgcaggcgt cacgcatccg actgcgatcg
     7261 ccccagcact gatcgggccg gttgttgatg cggatttccg gcttgtgata gccgaggacg
     7321 cacaggattc ggtgccataa aggcatccgt tccgggaaga tcggaagccc ggtgttgccc
     7381 cgcagatcat cgccatcagc catggttcgc ctccttcggt gcagcggcga ggcaggcaat
     7441 gagttcggaa aacatttcta gtttcgcaca aacgccgact agtccgacgc ggcgagcctg
     7501 cgattgtgtt tctctgatgc cgtattggct aatcgtctgc ccgtcaggag ccgtcaccca
     7561 tgcgccaact ggcggttcgc ttcctccgaa cgtccaccca tcaggcgcca ccccttcctg
     7621 cggctgcgcg ggtgtcgggg atagggctgc ttcaccttct ttcaccgcgt ctgcatcgct
     7681 agcaccacga agcttcgcgc tcaggtacgc ggctatggcg gcggcattct ccggtgcccg
     7741 catcagattg acgcagacgg tttccatcac ccggcgttga tctgcgagcc cctcagcagc
     7801 ctcttccatc aactgcgggc tcttcacgcg gttcatgtcc cgcaggtatc gagcgcggcc
     7861 ggtaagccgt tcgatcaacg tcttatgatc atcctcttcg tccgagcccc tcgcatattc
     7921 gctcgactgg ggttgcgtgt cggtcattga gccgtctccg atccattgat ctggcgcgca
     7981 ttgctcgcga gcattttgct cccgttgagg tagatgcgga cgccgttgaa aatctgcttg
     8041 gccagcatcg aacgagcgat ggcgtcgttg acgctgatcg accccgaacg aagcgcgtcg
     8101 aggtcgccaa tcagcccttg aatgacgccc tcaagcccaa gctgggccga cacgggcgcg
     8161 gactcgtaat cacgcgatgg catgacgctt gctccaattc tggcgggcgg cttcgtcgtg
     8221 cagggcttcc gccaagctga agccgcgaat gacgttccgg cggtctcgga aggagccatc
     8281 ggtgggcttg ccgatgccct gcgtgtactg gccttcgcag ccagtgcaga ccggctctat
     8341 ctggctcacg cgccccggca tgttccagcg cgtgcagatg aagcgctgac agatgacgca
     8401 ggtgaatccg gccttgctgt cagacatcgg ccttcctcca aatgatcggc tgttggtcgc
     8461 gcgggatgcg gtgcttgcct cgcgccatgg gatgcgtagg cgcgccgctc ttcgtcgtgc
     8521 cccagcacca gagatccggg tacggaggct cgccggtctg gatctcctcg acgacgtgct
     8581 cgatccactc ctgatcccag gcgatgttgc cccagcatac gaagacctgg tcggcagcct
     8641 tcgccatctc gacgacccga ggcagattga cgaagtggag ctggtcgcga tcggaccagt
     8701 cgagccccgc atcgatcgcc tcgacccgct tgcggcactc ggccgggctc gacgagcaga
     8761 acgggtagag gttgccagcg tcgtagcgac cgaagccgaa gagctggaac catgcgttcc
     8821 accaaagcga cgtcgggtcg tcctgcagcg cgttggcggt cgacggattg catccgatca
     8881 cgaaggcgcg aggccccggc ccccaggcgc gtgagagcag gatgcgttca catcccgcaa
     8941 attcggcgct ccggcgcatg acgggatcgc cgagcaggtc gagcagctca ctcatttcat
     9001 ggccctcgcg accaggatca gattgccgat caggaagagc gcgctgccga ccgcgaagag
     9061 gatggccggc agcgcggcaa tgaggcggtc gctcaccaag ggttccttcc catcagggcg
     9121 gaggtcaccg cgtgctcccg catagattgc gagcgccgct tcatctcagc tgccagctcc
     9181 cgttcaaccc gagcgatcgt atgcgggtgg ccgtagagcg tgccatctgg cccctggatc
     9241 atcttgggat cggctatctg gcggaatcgg atgttttgcc ggtggccccg tgcgcggcgc
     9301 cgccgggcgc gagacggcga gcggactcct gaccaatcct caaagaggac cgtcattacg
     9361 tcgctcgcca cgagcctgat gccgggcacg aacaaaggct tcgtcagcct gacggcgtca
     9421 aagaagggcg cgctcatgcc cactgacctc cgcgctttgg cttgtggggc atcggtggca
     9481 acggcgtcca atgcgtgtgg tagccgggcc agctgctatc gagcggcgtg cccacccagg
     9541 gggcctcggt gaccggaaac ttccaccaca ggacgtcccc gagatcctcg taccagtgac
     9601 gttccgcgcg cgctaaggcg ccggtatcgc ccggcgcgat gaacgcgaac cagatccgct
     9661 tgtccggatc gcatccacgc gtcttcgcga aagcgtcttg gatcaggaat cggccggcct
     9721 ctgcgagctg cgtcgtgtag ccgcacccat tcggcctcca gaaggcagac cattctgccg
     9781 accagatccg gaccatacgc ccagcgggca cgcgatctgg gttgcgcttg tagtgctcga
     9841 gcgagcgcaa ttcgggatcg cctggcacac cagacggccg gcgccgccac gacattgggt
     9901 gatgctggcg cttcatgccg ccctcgcggg cgaatgagga atgcctagct gagcgagcgt
     9961 gacggacccg tggccccgct cggccgcctg tttcatcaga tgcaggatca gcgtgtcggc
    10021 attgccctgc gaccgtgccg ccacatagga ccagtccatc ggcaggctgc gctcggctgc
    10081 gggcggcaga tcggcagtgg tgccgtattc gcccggcgca tattcgactg tggcaaggaa
    10141 cggcatgccg cgctcacggc actgcttggc aaggtccagc agcgcaggcg cgacaacgtc
    10201 gtcgtagaat tgctcgctca tgcggtcctc ccgatctgat cgatgttccc gcgctcgacg
    10261 cggaaggtga cgggaacgac gaaggggttg gcatcccagc tctcggcgcc gtggaggtgc
    10321 tgccaaatgc cggcgaaccc gcagtaatgg gttccagcgt cggatgcgta ccagccgccg
    10381 tcgccgtccc agacgagcct gtcgactcct tcggcctgcg cgtccgcctc gctgatcaac
    10441 tgcagctgct cgacgcgcac gtcctcgacg atcagcgtca ggcgcgaggc cgagcggggc
    10501 atgaagcgcg ccaagcgccg gtgccaagcg gtagttcccg ggtcggcgct atggcgcgcc
    10561 ttgcgaaacg ctatgggcgg atcgaagcgg atggcgtcgt cgtcgggcac gaacttccac
    10621 ttcggacgtc cgcccttcgt cactacgccc gccgcctgct cccagtggcc ctgctggtaa
    10681 tagctctccc gcacgtagag gcgatcaccg gcggcatagc cgatattggc catccatccc
    10741 gtgcgctctg cgggcgcctg ccattcgaag gcgtcagcct cccagaacca accagtcccg
    10801 tccaggtgtc ggaacccgga cgcgccttgc agcgcacggg ccatgtgctc ggccgccagc
    10861 gtgaaagccg gccgctctgg cgtggcgaat acccgcgccc gcgacagcag ccgccgcgtc
    10921 tgcgtcttgc ggccggcgag cagggcgcgc accatcggcc ccgagaatat gatggggcga
    10981 tcagtcatgg cgttcacaat tgatccggca gaagcggttt acgatttccg ttccagcacc
    11041 gtggttttct atttggagga tggcggcgcg cgcgttcggt gcggtgtttg ccaattggcg
    11101 ctggtggacg actatggggc cacctcgacg aaccaggtcg agatgctcga ggtcttccgc
    11161 gagaattgcg tcgcgattgg taaggctgcg gagcgcaaac accgcggcgg tttgaccgag
    11221 ccgaatggcc acatcctcgt aagaacgggg gatcggtagg ctccggatca tgccggccac
    11281 cccagagcgt gcagctgcgc gatccgctgg agagcctggt cgggcgtcat ccgcgagtag
    11341 aggccatctg tcgaaggcgc ctcgtgtccc aagccacggc ggaaggcccg cgtcggctcg
    11401 ccaaggacca catcgcggaa ctgctggatc acgacggcgg tgccgcgctg gacgttcgcc
    11461 agcgtgtgga tctgatccga tcgcgtcatg taggcggtgc cggcaccgtg aacgccgcga
    11521 tcccagcccc ccagccggac ttcgcgcttc caggtgaagc cgtcaccgcc gttcagcggg
    11581 gtcgaatagt cgaactgctg atagaccgtg ccctgcccgg catcgcactc ggcccagcgg
    11641 cgctcgacga gcccgccggc gagcacatgc gtcgtgaagc catatcggtg atcgtgtgga
    11701 acgacgagct ctgggctctc ggtacactca cccgcgaaga agtaggcctt gagcgtcagg
    11761 gcctcggagc ggtgcaggca gagatagtcg aggcccttgg cgtggaagtc gcgataggaa
    11821 ttcgcgcaca tagcctggag cgcctcgtca tcgaggctcg gaataaagcg atcggtcatg
    11881 gaacgggacc tcgttgaggg accaatagaa ggcagccgtc ccggcggcgc ccgaccagaa
    11941 ggtccaatgt gcttctcctc caccgaacaa gatggcgagg atatctacga gcaggatcgc
    12001 agctaagccg cagactgcag cccgggcata tttcccgacc acggtcatgc tgccagaccc
    12061 cgctcgctgc acgggaagtg aaccccaagc caatccccga gctgtgcccg gtgctcgcct
    12121 atcccggctc cgtccgtctc gctgaccttc atgatctggg cgaggccgcc gcgggacgcg
    12181 cgccacatgg tatcgcgata gagcgcaccg ccggcgaaca tgaccgcctc ggcctcagcg
    12241 aaaacgtcgg caatggcggc atggtccgac gtgcacatgc tcagcaggtc gtccgcgcgc
    12301 cgtgacgtca accgctgctc atacggcaga atggcgatgt cgacggggat gaatccgtgc
    12361 tgcgcgctca gaacccttac gacgagctca cctgatcgca tggcggcaac cgcagcaggc
    12421 agttcggcaa gccgcgcccg cagcgtctgc cacatgggtc cgtcgtaacg atcgatggcc
    12481 ggcataggcg ctgggttcgg gcgcttggtg gcgcagcaac tcatgatgag gagcttcatg
    12541 ccggcacccc atcgtgctgg acgccgtcga gcaggcggcc ggctgatttc ttgccgacgc
    12601 ggtagaccag gtcggtcgca tgttcggtga agctgtggcc tcggacgcga gcagcgcccg
    12661 catcgtcatc gaaaacgaca tagcggctca tccagtcagc cacgttgccc cagcggtcgg
    12721 tgacaggtcc gtctcgcgcg atccgagcgg cgctcatggg tgcccactcg ccccattgct
    12781 tgaagaggaa cgccaccaag gcagcggcgc attgatcgcg cagcgcccgg acccagtccg
    12841 gattaatggg gcgtgcagct gcgccgctct cgcctccgac gatgatccag tcgagatgct
    12901 ccagccattc cttgcggaga accacgggcc cgagcagtgg ctccatcgat aggccaaccc
    12961 agggtatgcc tagcttagcc ttcagcgcga tgaggcgcgg gacgtcgcga tcggcttcag
    13021 cctggttgac gacgctgatc agcagtccgg cgtgggccgg ccaagaggcc ccaatttctg
    13081 caaggcgctt ctcgacgacg gagacccgct tggtgacgat ctggatagcc aaccgattgc
    13141 acgacacgat gtgttgccat gcctcggcga accattcgac cggcacctcg atgtcgaaca
    13201 ggtcggacat cgactgaatg aatacgcgac gacgagaggt cgcgtgcatc tgcccgatat
    13261 agtgatcggc ggcccaccac gaataatcgt tgtccaggcg gtggatcagc ttcaccgcgc
    13321 tcttgatctt ccgacgcggc acgccgacgc cccagatgcc gccgcccgtg cgccgcgacc
    13381 acacctcggc gtaacagtgg tcacagccag gcccgacctt cgtgcagccc caccagaagt
    13441 tgacggtggc atcggtccac tcgatcttgg tgtcagcgcc catggccgcc tccattcgcg
    13501 agcgccagta gggtatcgcc gtggcagtac ctcgagcgct ctggacacca gcattcgacg
    13561 tcatgctcgg cgagctcatg gatgcgcgcc agaacacgct gccggagacg gtggagcgcg
    13621 atgatctcgt gatcaccgaa cccgaggcgc cgcatcgtcc ggtacgagag ctggccagcc
    13681 agccacgtgc gatgcagccc tgttgctcga gcatgaccga agcggtcgga gcgaaacggg
    13741 ttcgcataga tcgtcccgcg gccaacatag cgcggaggcg ccgcacggcc ccgccggtcc
    13801 tctggcttgc gaggccggcg gtgacgacgt ggtgcgccct cagatcgcga cgcagccgcg
    13861 atcacgcaac gctctccgga acgacctgcg cgcgcttgag gcactcgatc atgccgaatg
    13921 cgagcgtctt gatgcggaga gc
//
LOCUS       DATGEM010000282        10455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_663144, whole genome shotgun sequence.
ACCESSION   DATGEM010000282 DATGEM010000000
VERSION     DATGEM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663144"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VKQ27_08835"
     CDS             complement(<1..882)
                     /locus_tag="VKQ27_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLY89076.1"
                     /translation="METLVAGKNAVLVAPPGAGKTTLVPLALLEAGWLGDGGVLMLEP
                     RRLATRAAATRMASLRGEAVGGVIGFRTRLDAAVSAATRVEVVTEGLLVRRLQSDPTL
                     DGVAVVILDEVHERSLESDLALALCLDLQRVLRPELRLVAMSATADGARLAGLMDASV
                     VESAGRMFPVAVSHAGRDVPGPRELPEAMARAVRAALAEHEGDVLAFLPGMAEIRRTQ
                     SALEGCGAVVLPLHGELPPAEQDRALRPAEGRRVVLATSIAETSLTVPGVRIVVDGGW
                     RRAPRLDPSTGLTRLTTL"
     gene            984..3011
                     /locus_tag="VKQ27_08840"
     CDS             984..3011
                     /locus_tag="VKQ27_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HLY89077.1"
                     /translation="MLRLLTAILLLLPALGTTRASALESNRFTSPRDTASLVSEVDTV
                     VPGKPFRIGLRLQLAPGWHTYWRNPGDAGAPPDLQLTLPHGATATPIDWPTPRRIAEG
                     PLMTYAYSGDLLLPVTITATGGDTAVRTHAEWLVCREICVPEEADFQLDLPAGTGQPS
                     AQAPLFAALARQLPRPSPWHAVIGNDGTLLLQGPELTPATVVDAWFIPNASGVILDSA
                     AQPLTVWHGGFTLGLRPAKGFQASAPLTGVLSVRDRSGLETDVDLNAQPGAVPVPPPA
                     ISLNRTLLLAFIGGLILNLMPCVFPVLALKVIALAGAPRGKARWQAVAYTAGVLVAFA
                     GLGATLLAARAAGSATGWGFQFQSPVFVAAMAWLLFAIGLNLSGVFAVGSGLVRTGHG
                     LATRPGQLGSFFTGLLAVVVATPCTAPFMGVAIAAGLAAPPGVTMLVFVVMGLGLAAP
                     YALLASAPALGRLAPRPGRWMELLRQILAFPMYGASAWLVWVISQEAGPTGVLATSAG
                     LVLLGFAGWVLGITQNATDKGRRIGQSAAATAALAALAVLSGIATAPPVSADAAASGA
                     EPFSSSRLAALRAEGRPVFVDMTAAWCVTCLVNERVAIDTTAVRQAFAEHHVAYMRGD
                     WTRQDPHITDYLRENGRDGVPLYVYYPPSGQPQILPQILTENTVLSELNRG"
     gene            complement(3129..4280)
                     /locus_tag="VKQ27_08845"
     CDS             complement(3129..4280)
                     /locus_tag="VKQ27_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HLY89078.1"
                     /translation="MASIGRILTTHVGSLPRPHDLLDMMKASIAGRAVDEAVYRTRIR
                     DAVAACVRKQVACGIDVVCDGEQSKPGFFTYVRERLDGFEPRPDRPRTTFGAEVAAFP
                     EYYAEYFKAAMTGGAVAPVVPLVCTGPVRYRGGAALRRDIENLQAALAGVPHVAAFMP
                     AVAPSGVGENEYYNTDEAFFHAVGEALHEEYQAIVDAGFLLQIDDPFLSDLFGDPAFD
                     ARQRVERAEIYVSALNASLRGIPAERVRYHTCYGINHGPRIHEAALADVAAYMLRVDA
                     GAYSFEAANVRHEHEYHLWETVKLPQGKVLIPGVITHSSNIVEHPEWIAERLERFARL
                     VGRENVVAGADCGFSSQATYHTEVHPTVIWAKFEALRDGARIASERLWR"
     gene            complement(4297..5520)
                     /locus_tag="VKQ27_08850"
     CDS             complement(4297..5520)
                     /locus_tag="VKQ27_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89079.1"
                     /translation="MKFLFAHQNFPGQYVHILQHLVAKQQHDLVFLSEPNRNRINGVR
                     AIPYPKPSLPATEAHVGARELDAALRRAEVVQATAMNLKSLGFEPDIILGHHGWGELL
                     NLCDVWPGVPILGYFEFFYRIDGIDVNFDLEFPTQVSDYARIRAKNAVNLLALNLGQH
                     GQTPTQWQLSTYPEWAHPFITLLREGVKLDVCKPDAQARRRNLVIGKTTIKPNEKLVT
                     YVARDLEPYRGFHMMMRAIPHLLRARKDIRVVMVGADGISYGAPPQKGTWRETMLAEL
                     GDSIDPDRVLFPGRIEYNTYIAMLQRSDAHVYFSYPFVASWSLREALAAGCFVVGSDT
                     TTVREFVAHEQNGMLVPFFDPKALADTLLRGIEDTALSRRLRDNARKYAEKHLAMDDY
                     LKRYDALIAKVVGRK"
     gene            complement(5567..6547)
                     /locus_tag="VKQ27_08855"
     CDS             complement(5567..6547)
                     /locus_tag="VKQ27_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89080.1"
                     /translation="MSEAGTTNPNATAEQGAAQNRVTELKVSGHLMTLDTGVFAVFQV
                     PGSPPPGASTGLPGVRISLPPGAAERPDDLCISTFLNDGWISGHDGAALVRVAKGPAQ
                     VLVTVYQAPTASPDSAPRLQVMRLGAEQAGEARPAAAQAAVPILSADAADVVVHVQGV
                     GDQAAGINEWAGAKGSRRWIEGFSLTPHQDLDPADIEYQAVLGRGWLSPWIEGGKFCG
                     SRGMALPLLGLKVRLKGAAAKNYDCKYSASFVDGSAVGPVSGGETCEAESLAALEAFQ
                     ISIQQRGGAVAGAAPKAPAAATPKAPAAVRTRPGTPKPAAKRAAAKRASR"
     gene            complement(6623..9424)
                     /locus_tag="VKQ27_08860"
     CDS             complement(6623..9424)
                     /locus_tag="VKQ27_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89081.1"
                     /translation="MAREAFERGQAAAASGHGDEARRWLDRACRLAPTDRMLTLMLAT
                     ACLGYDDHRAAALFAMVTAEHDVREAWLGLASARRRLGDRAGAAEALAAALRRHVVEP
                     GFAALADAIAREVGAGGWLGVGRNGKLEEHILGGPVETSRLIGYPIDAAAIAATVGCV
                     SSQNGGLVGWAWHQGDPGTDPSLTIRPVTGRGRLVITASDLAHQVANGGLLARPRGFA
                     VPAEALQHMRGLLHVLGRDGKDLHGSPLDPRMELDAGQAAALALAVRYPAGRTARRPP
                     QSTAPMAMPVAMRVPRTAHVEPRRRPSATVVIPVYGGMTQALACIDSVGATGERPLVI
                     DDASPEPALADVLDDLARCHRIRLIRNRRNLGFVASANLGMRSAPGRDIVLLNSDTLV
                     ASGWLEEIRGIAYSAPDIGTVTPLSNDASILSYPDVQRRNPVPALGDTRLLDALARRA
                     NGGAAIEIPVGIGFCLYVKRACLDDVGVFRDDVFAQGYGEENDFCLRARHRGWRHVAA
                     PGVFVAHVGGHSFGSAGRHLRARNEVLLERLHPGYGGLVDAHTLADPLAEARRRLDLA
                     RWRAGRRRGSRAVILISHLAGGGVERQISVSVARHREAGHRAIVVRPSHAPDGSHCAT
                     VSDGTANGFPNLRYRLPDELPALQRLLATERPLMVELHHLVGHHPSVVELIGRLGLPY
                     DVHVHDYAWLCARVALVGPAGRYCGEPEVSVCEACVADGGNLIDEEIAVAALRQRSAR
                     LLAGARRVYTPSEDAAARIRRHFPRTRPVARPHEDDAAIVDLAAAPRRGRNRICVIGA
                     IGIHKGYQIVLDCARDAAERGLPLEFVVVGHTIDDGRLMATERVFVTGEFAPEEAIPL
                     IRAQHATLALLPSIFPETWCLSLGEAWRAGLRVAAFDIGAQAERIRRTGRGFLMPLGL
                     PTPAINNMLIAASGLSAKE"
     gene            complement(9457..>10455)
                     /locus_tag="VKQ27_08865"
     CDS             complement(9457..>10455)
                     /locus_tag="VKQ27_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HLY89082.1"
                     /translation="PANPVDGESWLRRAALAGDPEAAAVVGDLYARGGKLPPNYAEAA
                     IWFRHAADAGHRGAARALGLLHLTGAGVPRDSEEAAKWFRLSAQAGDRESRVDLANLL
                     LQGQGDADDALRTREWFEHAAASGDLVAAFNFGVCLAEGVGVERDDIKAAQWLRRAAD
                     GVVNAQYWYGRMLVEGRGQDANPEDGRAWIKRAADVGMVEAEVMLADLLISGRGGPKD
                     HAGAMVLFEKAAGRGHVGAMFAMGALNGGGHDVPTDRLAAQRWFQAAAERGHAYAQMM
                     LGRYLARGLAGERDIERARMWSERALAQGLQEARADIAALPQQAEPQPAEAQAASR"
BASE COUNT         1750 a         3965 c         3082 g         1658 t
ORIGIN      
        1 cagcgtggtt agccgggtca gcccggtgga cggatccagc cgaggcgccc gccgccaccc
       61 gccatccacc acgatccgta cgcccggcac cgtcagcgag gtctcggcga tcgacgtcgc
      121 cagcaccacc cgccgcccct cggccggccg caacgcccgg tcctgctctg ctggaggcag
      181 ctcgccatgc agcggcagca ccaccgcccc gcacccttcc agcgccgact gcgtccgccg
      241 gatctccgcc atcccaggca ggaacgccag cacatcgccc tcgtgctcgg ccagcgccgc
      301 ccgcactgcc cgcgccatcg cctccggcag ttcccgaggc cccggaacgt cccgccctgc
      361 atggctcacc gccaccggga acatccgccc cgcgctctcc accaccgagg catccatcag
      421 cccggccagc cgcgccccgt ccgccgtcgc cgacatcgcc accagtcgca actccggccg
      481 cagcacccgc tgcaagtcga ggcacagcgc cagcgccaga tcggactcca gcgaccgctc
      541 gtgcacttcg tccaggatca ccaccgccac gccatccagc gtcggatcgg attgcagccg
      601 gcgcaccagc agcccctcgg tgaccacctc cacccgcgtc gccgccgaca ccgccgcatc
      661 cagccgggtg cggaacccga tcactccgcc caccgcctcg cccctcagtg acgccatccg
      721 cgtcgcagcc gcccgggtcg ccagccgccg gggctccagc atcagaaccc caccatctcc
      781 cagccacccc gcttccagca gagccagagg taccagcgtc gttttgcctg ctcccggcgg
      841 cgccaccagc acggcatttt tcccggccac gagcgtctcc atcaaagcag gaagaacttc
      901 ggtgaccgga aggtctggca acatgctgtt cgtcgggtgc attctgccca tatgcgcatg
      961 aattcaccgg tgcggggaaa tcgatgctgc ggctgctgac tgcgatcctg ctccttctgc
     1021 cggccctcgg gaccacccga gccagcgcgt tggagagcaa tcgcttcacc tcgccgcgtg
     1081 acaccgcgtc gctggtctca gaggtcgaca cggtcgtccc cggcaagccc ttccgcatcg
     1141 gcctccgcct gcaactggct cccggctggc acacctactg gcgcaatccc ggggacgccg
     1201 gcgccccacc ggacctccag ctcaccctcc cccatggcgc caccgcgacc ccgatcgact
     1261 ggcccacgcc gcgccggatc gccgaaggcc cgctgatgac ctacgcctac agcggcgatc
     1321 tgctcctgcc ggtgacgatc accgcgaccg gcggcgatac ggccgtcaga actcacgccg
     1381 aatggctggt ctgccgcgaa atctgcgtcc ccgaggaagc agacttccag ctcgacctcc
     1441 ccgccggcac cggccagccc tccgcgcagg cccccctctt cgctgctctg gcacgccagc
     1501 ttccccgccc ctccccctgg cacgcagtga tcggcaacga cggcacgctc ctgctccagg
     1561 gcccggagct gacgccggcc accgtggtcg acgcctggtt catccccaat gcatccggcg
     1621 tcatcctgga cagcgccgcg cagcctctga ccgtctggca tggcggcttc accctcggcc
     1681 tgcgtcccgc caaaggcttc caagcctcgg cccctctgac cggcgtgctg tccgtgcgcg
     1741 accgcagcgg actcgaaacc gatgttgacc tcaacgcgca acccggcgcg gtgcccgtgc
     1801 caccgcccgc aatcagcctg aaccgcaccc tgctgctcgc cttcatcggc ggcttgatcc
     1861 tgaacctgat gccctgtgtc ttcccggtcc tggcgctcaa ggtcatcgcc ctcgccggcg
     1921 ccccgcgagg caaggcgcgg tggcaggcag tcgcctacac cgccggcgtg ctggtcgcct
     1981 tcgccggcct cggcgccacg ctgctcgccg cccgcgcggc cggttcggcg acaggctggg
     2041 gtttccagtt ccagtcaccg gtgttcgtcg ccgccatggc ctggctgctg ttcgccatcg
     2101 gcctgaacct gtccggcgtc ttcgcggtcg gctccggcct tgtccgcacc ggccacggcc
     2161 tcgccacccg cccaggccag cttggcagct tcttcactgg cctcctggca gtggtggtcg
     2221 cgaccccttg caccgcaccc ttcatgggtg tggcgatcgc tgccggcctc gccgcaccgc
     2281 ctggcgtgac gatgctagtg ttcgtcgtga tgggcctcgg cctcgccgcc ccttacgctc
     2341 tgctggcatc agccccggcg ctgggccgcc tggcaccgcg ccctggccgc tggatggaac
     2401 tccttcgcca gatactcgcc ttcccgatgt acggcgcgag tgcctggctg gtctgggtca
     2461 tcagccagga agctggcccg accggtgtgc tcgccacgtc cgccggcctg gtgctgcttg
     2521 gcttcgccgg ctgggtgctc ggcatcaccc agaacgcgac cgacaagggc cgccgcatcg
     2581 gccaatccgc cgcagccacc gccgcactgg cggcgcttgc cgtgctcagc ggcatcgcca
     2641 ccgcgccacc ggtgtcggcc gacgcagccg cttcaggcgc cgaacccttc agctccagcc
     2701 gcctcgctgc attacgcgcc gaggggcgtc cggtattcgt cgacatgacc gccgcctggt
     2761 gcgtcacctg cctggtcaac gaacgggtgg ccatcgacac aacggccgtt cgccaggcct
     2821 tcgccgagca ccatgtcgcc tacatgaggg gcgactggac acgccaggac ccgcacatca
     2881 ccgactacct gcgcgagaac ggccgcgatg gcgtgccgct ctacgtctac tacccgccca
     2941 gcggccaacc gcagatactg ccgcagatcc tcaccgagaa cactgttctc agtgaactga
     3001 accgcggcta agcccagtgc tcaccgcgtc ggactgtgct attgcgaccc cggcgaaggc
     3061 cgggggaagc aatctcggac ggcgtcgcct ctgtcgcgga ctcgtaccga gacgcctcca
     3121 atacagctct accgccacag ccgctcgctg gcgatccgcg ccccatcccg cagcgcctcg
     3181 aacttcgccc atatcaccgt cggatgcacc tcggtgtggt acgtcgcctg cgacgaaaat
     3241 ccgcagtccg cgcccgcgac gacattctcc cgacccacca gccgcgcgaa ccgttccagg
     3301 cgctcggcga tccactccgg atgctcgacg atattcgacg agtgcgtgat cactcccggt
     3361 atcagcacct tgccctgggg cagcttcacc gtctcccaca gatggtactc atgctcgtgc
     3421 cgcacattcg ccgcctcgaa gctgtatgca ccagcgtcta cgcgcagcat atacgcagcg
     3481 acatcggcga gtgctgcctc atggatccgc ggtccatgat ttatgccgta gcaggtgtga
     3541 tagcgcacac gctccgccgg aatcccccgc aggctcgcgt tcagcgccga gacatagatc
     3601 tccgcccgct ccacccgctg tcgcgcatcg aacgcaggat cgccgaacag atcggacagg
     3661 aacggatcat cgatctgcag caggaacccg gcatcgacga tcgcctggta ctcctcatgc
     3721 agcgcctcgc ccaccgcgtg aaagaacgcc tcatcggtgt tgtagtattc gttctcgcca
     3781 acgccgctcg gcgccaccgc cggcatgaac gccgccacat gcggcacgcc agccaacgcc
     3841 gcctgcagat tttcgatatc gcgtcgcaga gccgcaccgc cgcgataacg caccggaccc
     3901 gtgcacacta gcggcaccac cggcgccacg gcaccgcccg tcatcgccgc cttgaagtat
     3961 tcggcataat actccggaaa cgccgcgacc tcggcaccga acgtcgtgcg cggccgatcg
     4021 ggtcgcggct cgaacccatc cagccgctcg cgcacatagg tgaaaaatcc cggcttcgac
     4081 tgctcgccgt cgcagacgac atcgatgcca cacgccacct gctttcgcac gcacgccgca
     4141 accgcatcgc ggatgcgcgt ccgatacacc gcctcgtcga ccgcacgccc tgcgatcgac
     4201 gctttcatca tgtcgagcag atcgtgcggc cgtggcaggc tccccacatg cgtcgtcaga
     4261 atgcgaccga tgcttgccat cgcggtcagc tccccattat ttccgcccca ctaccttggc
     4321 gatcagcgca tcatacctct tcaggtaatc gtccatcgcc agatgcttct cggcgtattt
     4381 acgcgcgttg tcacgcagcc gccgcgacag cgccgtgtct tcgatcccgc gcagcagtgt
     4441 gtcggcgagc gccttcggat cgaaaaacgg caccaacatg ccattctgtt cgtgcgccac
     4501 gaattcccgc accgtcgtcg tgtcactgcc aacgacgaag cagcctgccg ccagcgcttc
     4561 acgcagagac cacgaagcga caaatggata gctgaagtaa acatgcgcgt ccgagcgctg
     4621 cagcatcgcg atataggtgt tgtattcaat acggccagga aacagcaccc gatccgggtc
     4681 tatactgtcg ccgagctcgg ccagcatcgt ttcgcgccac gtccccttct ggggcggcgc
     4741 cccatagctg atgccgtcag cccctaccat gaccacgcgg atatccttgc gggcacgcag
     4801 cagatgcggg atggcccgca tcatcatgtg gaagccgcga tacggctcca ggtcacgtgc
     4861 gacgtaggtg accagcttct cattcggctt gatcgtggtc ttgccaatga ccaggttgcg
     4921 gcgccgcgcc tgcgcgtccg gcttgcacac gtcgagcttc acgccttcgc gcagcaacgt
     4981 gatgaacgga tgtgcccatt caggataggt ggaaagctgc cattgcgtcg gcgtctggcc
     5041 gtgctggccc agattcagcg ccagcaggtt gacagcgttc tttgccctga tccgggcgta
     5101 gtctgacacc tgcgtaggaa attccagatc aaaattaacg tcgataccat cgatccggta
     5161 gaagaactcg aaatagccaa gtatcggcac cccgggccag acatcgcaca ggttcagcag
     5221 ctcgccccag ccgtggtggc cgagaatgat gtccggctcg aagccgagcg atttcaaatt
     5281 catcgcggtg gcctgcacga cctcggcgcg gcgcagagcg gcatcgagtt cgcgtgcgcc
     5341 aacatgggcc tcggtcgccg gcaatgatgg cttcgggtaa gggatggccc gcaccccgtt
     5401 gatgcggttc cggttgggct cgctcaggaa caccagatcg tgctgctgct tcgcgaccag
     5461 atgctgaagg atatgcacgt actgcccggg gaagttctgg tgcgcaaaca gaaatttcac
     5521 gccgcaacct tgtctgccga acggatccga gcgcagccgc ctggtttcag cgcgatgctc
     5581 gcttcgcggc ggctcgcttc gccgccggct ttggtgtgcc aggcctcgtg cggacggctg
     5641 ccggtgcttt cggcgtagcg gctgccggtg ccttcggtgc ggcgcctgcc accgcaccgc
     5701 cacgctgctg gatcgatatc tggaacgcct ccagtgccgc caggctttcc gcctcgcagg
     5761 tctcgccgcc ggataccggg cccaccgcgc taccatcgac gaagctggca gaatacttgc
     5821 agtcataatt cttcgcggcg gcgcctttca gccgcacctt cagcccgagc agcggcagag
     5881 ccatgccccg gctgccacag aacttcccgc cttcgatcca cggcgacagc cagcctcggc
     5941 caagtaccgc ttggtattca atatccgccg gatcgaggtc ctgatgcggc gtcagactga
     6001 agccttcgat ccagcgacgg ctgccttttg cgcccgccca ttcgttgatc ccggctgctt
     6061 ggtccccaac accctgcaca tgcacaacca catccgcagc atccgccgac aggatcggca
     6121 cggccgcctg cgcggcggcc ggtcgcgcct cacccgcctg ttccgctccc agccgcatga
     6181 cctgaagtcg cggtgcgctg tccgggctcg ccgtcggcgc ctgatacacc gtcaccagaa
     6241 cctgcgcggg gcccttcgcc acacgcacca gcgccgcacc gtcgtggccg ctgatccagc
     6301 cgtcgttaag gaatgtgctg atgcagagat cgtccggccg ctccgcagca cccggcggca
     6361 atgaaatgcg cacgcccggc aagcctgttg atgcgccagg aggcggtgag cctggcacct
     6421 ggaacacggc gaacacccct gtgtccagcg tcatcagatg ccccgagact ttcagttcag
     6481 tgacgcggtt ctgcgcagcg ccttgttccg ccgtcgcgtt gggattggtt gtgccagctt
     6541 cggacatgca gggtctcgct ctccggttgc ctctgtcagc agcgacttga ccgcttacgc
     6601 gatacaacca ttcgtagcaa agtcattcct ttgccgatag acccgaagca gcaatgagca
     6661 tgttgttgat ggccggcgtg ggcaggccaa gtggcatcag aaatccacgg ccagtgcgcc
     6721 ggatgcgctc cgcctgcgcg ccgatatcga atgccgcgac acgcagtccg gcacgccagg
     6781 cctcgccaag gctcaggcac caggtctcag gaaagatcga gggcaacaag gccagtgttg
     6841 cgtgttgcgc acggatcagc gggatcgcct cctccggtgc gaactcaccg gtgacgaaca
     6901 cccgctccgt tgccatcagg cgcccgtcat cgatcgtgtg gccaaccacc acgaactcaa
     6961 gcggcaagcc acgttccgca gcatcacgcg cgcagtccag cacaatctga tatcccttgt
     7021 ggatgccgat ggcgccaatc acgcagatcc ggtttcggcc gcggcgcggc gcggccgcga
     7081 gatcaacaat cgcggcatcg tcctcatgcg gccgcgccac cggccgcgtg cgcgggaagt
     7141 gccggcggat gcgagcagcg gcatcctctg aaggcgtgta gacacgccgc gctcccgcga
     7201 gaagccgcgc tgaacgctgg cgaagcgcgg cgactgcgat ctcctcgtcg atgagattgc
     7261 cgccgtcggc aacacaggct tcgcacaccg agacctcggg ttcgccacag taacgccccg
     7321 caggcccaac caatgcgacg cgcgcgcaca accaggcgta gtcgtgcacg tgaacgtcgt
     7381 agggtaagcc caggcggcct atcagctcga cgaccgaggg atgatgtcca accagatgat
     7441 gcagttcgac catcagcgga cgttccgtcg cgagcagacg ctgcaaagcc ggcagttcgt
     7501 ccggcagccg gtatcgcagg ttaggaaatc cgttcgccgt accgtcgctg acggtggcgc
     7561 aatggctgcc atccggcgcg tgcgatggac gcaccacgat cgcacggtgc ccagcctccc
     7621 gatgccgtgc caccgagacg ctgatctgcc gctcaacacc gccccccgcg agatggctga
     7681 tcaggatcac cgcccggctg ccgcgccgcc tgccggcacg ccagcgcgca aggtccagtc
     7741 gccgtcgcgc ctctgccagc ggatcggcca gcgtatgggc gtcaaccaac cccccatagc
     7801 cgggatgcag gcgctccagc agcacctcgt tccgtgcccg caagtgccgc cccgccgaac
     7861 cgaaggagtg gccgccaaca tgcgcgacga acacgcccgg ggccgccaca tggcgccagc
     7921 cacggtgacg cgcgcgcagg cagaaatcgt tttcctcgcc atagccctgg gcaaacacgt
     7981 cgtcacggaa cacgccgacg tcgtccaggc aggcacgctt cacgtatagg caaaagccga
     8041 tacccacggg aatttcgatt gccgcgccac cattggcgcg ccgggcaagc gcatcgagca
     8101 gtcgcgtatc ccccagtgcc ggcaccgggt tgcggcgctg gacgtcggga taactcagga
     8161 tgctcgcgtc gttggacagc ggtgtgacag tgccgatgtc cggcgcgctg taggcgatgc
     8221 cgcgaatctc ctccagccaa ccgctcgcaa cgagcgtgtc actgttcagc aggacgatgt
     8281 ctcgtccggg cgccgaacgc atgccgagat tcgcactcgc cacgaatccg aggttccgcc
     8341 tgttacggat caggcggatg cgatgacagc gcgcgagatc gtccaacacg tcggccaatg
     8401 ccggttccgg cgaagcatca tcgatcacca aaggccgttc accggttgca ccgacactgt
     8461 cgatgcaggc aagcgcctgc gtcatgccgc catataccgg aatgaccacg gtcgccgagg
     8521 ggcgccgccg gggttcgaca tgcgccgtgc gcggcaccct catggcaaca ggcatcgcca
     8581 tcggcgcagt cgattgcggc gggcgcctgg cggtccggcc ggccggatac cgcactgcca
     8641 gcgccagtgc ggcagcctgg ccggcatcca gctccatgcg cggatcgagc gggctgccat
     8701 gcaggtcctt gccgtcccgc ccaagcacgt gcagcagccc gcgcatgtgc tgcaaggctt
     8761 cggccggcac ggcgaagcct cgaggccgcg caagcaatcc gccgttcgcg acctgatgcg
     8821 caaggtcgct tgcggtgatc accagacggc ctcggccagt aaccggccgt atcgtcagcg
     8881 aaggatcggt gccggggtcg ccttgatgcc acgcccagcc caccagccca ccattctgac
     8941 tgctgacgca gccaacggtt gcggcgatcg cggcggcatc gatcgggtac ccgatcaggc
     9001 gcgacgtctc gactggacca ccaaggatat gctcctcgag ctttccattc cttccgacgc
     9061 caagccaccc gcccgcaccc acctcccgcg caatagcgtc cgccagtgcg gcaaatccag
     9121 gttcaaccac atgccggcga agcgcggcgg ccagcgcctc cgccgcgccg gctcgatcgc
     9181 ccagccggcg ccgcgctgac gcaagcccca gccacgcctc gcgcacgtcg tgctcggcag
     9241 tcaccatggc aaacagcgcg gctgcccggt gatcgtcata cccaaggcag gcggtcgcca
     9301 gcattagcgt cagcatgcga tcggtcggcg ccagacgaca ggcgcggtcg agccaccgcc
     9361 gcgcctcatc gccatgcccg gatgccgccg ccgcctggcc acgctcgaac gcttcgcgcg
     9421 ccaacccggc tgtgtcggat ggctcgcccg gtgcgctcag cgactggccg cctgcgcttc
     9481 ggccggttgc ggctccgcct gctgcggcaa cgctgcgata tcagcccttg cttcctgaag
     9541 accctgggcc agggcgcgct cgctccacat gcgcgcgcgc tcgatatcgc gttccccggc
     9601 cagtccacgc gcgaggtatc gtcccagcat catctgcgcg taggcatgcc cccgctcggc
     9661 cgccgcctgg aaccaacgct gtgccgccag cctgtctgtc ggcacgtcat ggcctccgcc
     9721 gttcagcgcg cccatggcga acatcgcgcc gacgtggccg cggccggccg ccttctcgaa
     9781 cagcaccatc gcacccgcat gatccttcgg cccgccgcgt ccggaaatca gcagatcagc
     9841 cagcatcact tccgcctcga ccatgccgac atcggcggca cgcttgatcc aggcgcgacc
     9901 atcctcggga ttcgcgtcct ggccccgccc ctcgaccagc atgcggccat accaatactg
     9961 cgcgttgacc acaccatcgg cggcccgccg gagccattgc gccgccttga tgtcgtcgcg
    10021 ttcgacgcca acaccctcgg caaggcagac gccgaaattg aaagccgcca ccaggtcacc
    10081 tgacgccgcc gcgtgctcga accactcgcg cgtgcgcaac gcatcgtccg catcgccctg
    10141 cccctgcaac agcaagttcg cgaggtccac acgcgactcg cggtccccgg cctgcgccga
    10201 caggcggaac cacttcgccg cttcttccga atcgcgcggc acgccggcgc cggtaagatg
    10261 caacagcccc agggcgcgcg ccgccccgcg atgcccggca tcggcggcat gccggaacca
    10321 gatcgccgcc tcggcgtagt tgggcggcaa cttgccgcct cgcgcataca aatcgcctac
    10381 gacggccgcg gcctcggggt ctcccgccaa cgcggcccgc cgcagccacg attcgccatc
    10441 gaccgggttg gcggg
//
LOCUS       DATGEM010000283        10011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1181056, whole genome shotgun sequence.
ACCESSION   DATGEM010000283 DATGEM010000000
VERSION     DATGEM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181056"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1194)
                     /locus_tag="VKQ27_08870"
     CDS             complement(244..1194)
                     /locus_tag="VKQ27_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLY89083.1"
                     /translation="MGLTIAPRQTPGTFLYAEPVAALEGLVADIAFLGVPYAQAYSYE
                     DITNDQSNGPTAMREASTRIIRSLERYDFDLGGPLYAGRAIRTVDCGDVRSEVGDAAG
                     HSRRTEQAVRAILAAGALPIVLGGDHGVPIPVLRAYEGRGPITLVQIDQHIDWRDEVN
                     GVREGLSSPIRRASEMAHVGEIFQIGLRATGSARQEEVDAALAYGAHLIPAWELHSVG
                     MEAVLARIPDGGRYYLTVDLDGMDPSIAPAVAAPCPGGVTMDQARTLIHGLVRKGRVV
                     GMDVVEITPRDDVNRITCITAGRMIVNLIGMAVRAGYFER"
     gene            1233..2549
                     /locus_tag="VKQ27_08875"
     CDS             1233..2549
                     /locus_tag="VKQ27_08875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89084.1"
                     /translation="MTTPRKMHFVAFLLAGPTAHHHGAWRHPEADLGVFTPEWHEHIA
                     RVLEAGKFDSLFFADILGLYDLYGGSFASILRCGGQMGLLDPIPPLSMMARVTSRIGL
                     GATLSSTFYPPYHLARSLGTLDLLSGGRVAWNVVASHGHLEAQNYGLDQLPPRDQRYD
                     FADEVVEAVVRLWESWDADALVLDKQRGIYADPDKVHYVNYQGRWIKTRGPLTVPRSP
                     QGRPVIMQAGSSPRGRDFAAKWGELVFTLQHSKPDMQAFYRDVKTRVVTHGRAPQDCL
                     ILPSIDIIVGETDAIARERQAYVNDLVQTEAGMAQMSGHVGVDLSRFPPDQPLANMEI
                     EAGSRGSLDVILQGTEAQGLTLGEAARRFATSELCPQIVGSPETIADHLQDLFESEAC
                     DGFILTPTLMPGMFEQFVRAVVPVLQRRGLFRQEYAGATLRDHLRD"
     gene            2559..3401
                     /locus_tag="VKQ27_08880"
     CDS             2559..3401
                     /locus_tag="VKQ27_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89085.1"
                     /translation="MKVAITGALGRLGRYVVAACTPHHTVRLIDLGPPADCHQADVRD
                     LPTLLTAFRDIDVVIHLGAIDKSVALDNATTMQVNAMGTWNVFEAAHQTGVRRVIHCS
                     SSSAIGVDQTNPTIPPAYLPIDEQHPAHPTDAYGLSKLCGERIAESFSRRGLEVIVIR
                     PCFVSFPDQAAFMAGEPGPAGRSEPMPYLRAYVGPEDCARGFLAAATAPYAGFETFFL
                     AAADTFATESTVPRLETLYGTAIPLRDPTLYRDNPRASPISHAAAQARLGWVPTTNWS
                     LDAK"
     gene            3559..3834
                     /locus_tag="VKQ27_08885"
     CDS             3559..3834
                     /locus_tag="VKQ27_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89086.1"
                     /translation="MVPPSNDALRQIIERDTDLVDTLNEYYGDATPDGGLDKVERELL
                     LDLLSRHYTGQLWPRTGGMEATRRFMANLQRAMAAAGWKLSGFALAA"
     gene            3962..5086
                     /locus_tag="VKQ27_08890"
     CDS             3962..5086
                     /locus_tag="VKQ27_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLY89087.1"
                     /translation="MTHPALDALLASTARPFEDATAMPPAVYTSSDILAVEQQRIFRR
                     EWICVGRASAIAEPGAYLTYEIAGQPMIILRDAACAIRAFSNVCLHRYSVLLEGTGRT
                     SAIICPYHAWTYGLDGALRGAPHMDRTPGFCRDNYRLPELRTETWQGWIYVTLNPDIA
                     PVAERLSKLTGMIHQYHMENYIETFREELVWDTNWKILAENFMESYHLPTLHRATIGP
                     ASKIDEMDCPPGFDTFNYHWITKDASLPIGNAHPDNTTLEGDWRRTVALMSIYPTQLI
                     SLTPGYFWYLLLSPRGTGQVHIIFAGGLAPEFISEPRGPEHMATLKALLDKVNAEDRS
                     GVEGVYRNAHAPLAAPGHLSWLERPNYDFARYLARQLAEG"
     gene            5264..6595
                     /locus_tag="VKQ27_08895"
     CDS             5264..6595
                     /locus_tag="VKQ27_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89088.1"
                     /translation="MEETVVAGGRIAGSSTLTRYQWLVFLVVWLGWTLDSADFGLYAL
                     VLRPALTELLGGNPPLTQVGSYGGILSMMGLLGWAIGGFVFGIYADYVGRVRALATSI
                     LLYSVFTALQGLSQGIWDFAIYRFIAGLGTGAELMVGIPLLAETLNSTHRAKIAGAMM
                     TGGALGTFLGAWVYGYIGSYGWRAVFFVGLLPALLLAIIRRRMIEPDRFATVLERRQT
                     IRAGQRVADDDSQFMRFVPLQLFSAEHRRNTIVGLLFGLGSLLAIWTSNIWLPTILSL
                     MIQKSGVTGSMAVPFVSHGIMLWSLGGIAGYCVFGFLADLLGRRGTITLYSLGTIAAG
                     LTLYLGLDSYYPYYPIVLPIFGFCVFGTFSGFAIYLPELFPTHVRSTAVGFTTGTARI
                     VTSFGPLVAGLMVGAFGGSFNRVTAVMTCMALFSIIAMAFARETKGAELPH"
     gene            6641..7330
                     /locus_tag="VKQ27_08900"
     CDS             6641..7330
                     /locus_tag="VKQ27_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89089.1"
                     /translation="MNFANKTVIVTGGTGALGRAVVGTLLAAGAQCRVPFVVDAEAAT
                     YPHKDRVTLIGHCELTNEADVARLYDGVTDLWASIHIAGVFAMGAVADTDKTGMLDML
                     NTNFVTCYLCSRAAVQNFSAAGGRIVNITARPGIEPRLGSGMAPYAAGKAAVAAFTQS
                     LAAEVAALSILVNAVAPSIIDTPANRAAMPKADHAAWPKPEEIAATIAFLASPDNKVT
                     TGAIVPVSGRT"
     gene            complement(7429..8616)
                     /locus_tag="VKQ27_08905"
     CDS             complement(7429..8616)
                     /locus_tag="VKQ27_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLY89090.1"
                     /translation="MSTSVQKPRGRQFFANPGPTNIPDSVLRALDRPSIDFHDPDFVE
                     VYDEAFAGVKRVLQTQHHLFMYNASGHGAWEASLTNLLSPGDTILVLESGYFSEEWAN
                     MARAHGLDVRLVGCDWRHGVDVAQLRATLTEDALAVIKAVCVVHNETATGMMLPLDEI
                     RAAIDAANHPALFLVDTISSLGSLDFRMDEWKIDCVVGGSQKGLMLPTGVSFTGVSPK
                     GLEAHARAKLPRFYFDWTLMEKRPQKSFIGTLPVNMFYGLSESIRLLEEEGLPNVFAR
                     HTRLAEATRRAVKVWAGNNGPQLFCTNPGRYSDSVTAIVMPEGHDADALRETARGRFN
                     VSLGGGLGALRGRVFRIGHLGDLNEPMLLGALSAVEMSLRINHVPHGKGGVDAAMEFL
                     SEG"
     gene            8802..9254
                     /locus_tag="VKQ27_08910"
     CDS             8802..9254
                     /locus_tag="VKQ27_08910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HLY89091.1"
                     /translation="MKRALLVREVLADCYCRLQPSAIHGIGVFAIRDIPRGKNPFQML
                     PRYARPGYMRITHEELEALPPGLARTIRALFVPTDGAMYIPTCGTNLVYLIAYLNHSN
                     VPNLCTRDGFNFFTRRKVREAEELTVDYRTYGADDLLPSPKSPLILNN"
     gene            9359..>10011
                     /locus_tag="VKQ27_08915"
     CDS             9359..>10011
                     /locus_tag="VKQ27_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89092.1"
                     /translation="MPDDVAAHAPADPIEQLPKAVYRTIIADLHALIPPPTLTDPELI
                     AERVHAAIAQIATMVPVNADEANIALRVVIAEANAKECARHARMYFNDTALLLKCEAQ
                     ANHCQRTANAARALLLRVQSARHKREANRTTCDQDAWTIHATQGYLLSANGERVSAPP
                     PPPTPEPPSPSPNAADDTSAPSHTEAELYAIMYPRRAAQIRAHGGMPPDARFDRPAP"
BASE COUNT         1792 a         3877 c         2632 g         1710 t
ORIGIN      
        1 cggattttgt tcatggacag tggccgcgtc ctggaggaca ccgccccaac gacattctcc
       61 acccaaccca cgcacccacg cgcgcgcgaa ttcctccgcc gcatccacgt ccccgtcgcc
      121 gtgtgccagt agctacatcc atcatcatgt cattgcgagg aacggagcga cgaagcaatc
      181 tcgaacgacc atgggtgaac ttcctgaatc cgcatcaccg ctccacccct cgcaacaagg
      241 gtgctagcgc tcgaaatatc ccgcccgcac cgccatcccg atcaggttca cgatcatccg
      301 cccagccgta atgcaggtga tccgattgac gtcgtcccga ggcgtgatct ccacgacatc
      361 catcccgacc acacgccctt tccgtaccag cccatggatc agcgtgcgcg cctgatccat
      421 cgtcacgcca ccaggacaag gcgccgccac cgccggtgca atcgacgggt ccatcccatc
      481 cagatccacc gtcagatagt accgcccgcc atccggaatg cgcgccagca cggcctccat
      541 cccgacagag tgcagttccc acgccggtat caggtgcgcg ccataggcca gcgccgcatc
      601 cacctcctcc tgccgcgcgc tgcccgtggc acgcagtccg atctggaaga tctcgccgac
      661 atgcgccatc tccgatgcgc gccggatcgg gctcgacagc ccctcgcgca ccccgttcac
      721 ctcatcgcgc caatcgatat gctgatcgat ctgcaccagc gtgatcggcc cacgcccctc
      781 atacgcccgc agcaccggta tcggcacccc gtgatcacca cccagcacga tcggcaacgc
      841 gcccgccgcc aggatcgcac gcaccgcctg ctcggtccgc cgcgaatgcc ccgccgcatc
      901 ccccacctcc gaccggacat cgccgcaatc caccgtgcgg atcgcacgcc ccgcgtagag
      961 cggcccgccc aggtcgaagt cgtaccgctc cagcgaccgg atgatccgcg tcgaagcctc
     1021 ccgcatcgcc gtcggcccat tgctctgatc gttggtgatg tcctcgtagc tgtaggcctg
     1081 cgcatacggc acgcccagga acgcgatgtc ggcaaccagc ccctccagcg ccgccaccgg
     1141 ttcggcatac aggaacgtgc caggcgtttg ccgtggcgct atggtgagac ccaccgctgt
     1201 tctcctctcc gcatgtcttt atggtccaag cgatgacgac gccccgcaag atgcatttcg
     1261 tggccttcct gctggcaggc cccaccgcgc accatcacgg cgcgtggcgc cacccggaag
     1321 ccgatctcgg cgtcttcacg ccggaatggc acgagcacat cgcccgcgtc ctcgaagccg
     1381 gcaagttcga cagcctgttc ttcgccgata tcctcggcct ctacgacctc tacggcggca
     1441 gcttcgcctc catcctgcgc tgcggcggcc agatgggcct gctcgacccg attcccccac
     1501 tctccatgat ggcgcgcgtc acctcgcgca ttggcttggg cgcaacgctg tcctccacct
     1561 tctatccgcc ctaccacctc gcccgctcgc tcggcacgct cgacctgctc agcggcggcc
     1621 gagtcgcctg gaatgtcgtc gcctcgcacg gccacctcga ggcgcagaac tacggcctcg
     1681 accagcttcc cccgcgcgac cagcgctacg acttcgccga cgaagtggtg gaagccgtcg
     1741 tccgcctgtg ggaatcgtgg gacgccgacg ccctggtgct cgacaagcag cgcggcatct
     1801 acgccgatcc cgacaaggtc cactacgtca attaccaggg ccgctggata aagacccgag
     1861 gccccctcac cgtcccccgc tccccccaag gacgcccggt gatcatgcag gccggttcct
     1921 ccccccgcgg acgcgacttc gccgccaaat ggggcgagct cgtcttcacc ctccagcaca
     1981 gcaaacccga catgcaggcc ttctaccgcg acgtaaagac gcgtgtagtg acacacggcc
     2041 gcgctcctca ggactgcctg atcctgccct ccatcgacat catcgtcggc gaaaccgacg
     2101 ccatcgcccg cgaacgccag gcctacgtga acgatctggt gcagaccgaa gccggcatgg
     2161 cgcagatgtc cggccacgtc ggcgtcgacc tctcgcgctt cccgcctgac cagccgctcg
     2221 ccaacatgga gatcgaagcc ggctcacgcg gctcgctcga cgtgatcctg caaggcaccg
     2281 aagcccaggg cctgacgctc ggcgaagccg cccgccgctt cgccaccagc gagctatgcc
     2341 cgcagatcgt cggctccccc gaaacgatcg ccgaccacct ccaggacttg ttcgagtccg
     2401 aagcctgcga cggcttcatc ctgaccccca cgttgatgcc aggcatgttc gagcaattcg
     2461 tccgcgccgt tgtccccgta ttgcagcgcc gcggcctgtt caggcaggaa tacgccggtg
     2521 ccacgctccg cgatcacctc cgcgactgac ccccacccat gaaggtcgca atcaccggcg
     2581 cgctcggtcg cctcggccgc tacgttgtag cggcctgcac cccccaccac accgtccgcc
     2641 tcatcgacct cggcccaccc gccgattgcc accaggccga tgtccgggac ctccccaccc
     2701 tcctcaccgc attccgcgat atcgacgtcg tcatccacct cggcgccatc gacaaatccg
     2761 ttgcgctgga caacgctacc accatgcagg tgaatgccat gggcacctgg aacgtcttcg
     2821 aggccgccca ccagaccggt gtccgccgcg tcatccactg ctccagcagc tccgcgattg
     2881 gcgtcgacca gaccaacccc acgatccccc cggcgtacct gccgatagac gagcagcacc
     2941 ccgcccatcc caccgacgcc tatggcctgt cgaaactctg cggcgagcga atcgccgaat
     3001 cgttctcccg ccgcggcctg gaagtgatcg tcatccgccc ctgcttcgtc tccttccccg
     3061 atcaggccgc cttcatggct ggcgagcccg gccccgccgg ccgctccgaa cccatgccct
     3121 acctccgtgc ctatgtcggc ccggaggact gtgcccgcgg ctttctcgcc gctgcgaccg
     3181 ccccctacgc cggattcgaa acatttttcc ttgccgctgc cgataccttc gccaccgagt
     3241 cgaccgtacc gcgcctggaa acgctgtatg ggacagcgat cccactgcga gacccaacgc
     3301 tttatcgcga caatccgcga gcctcgccga tctcccacgc cgccgcccaa gcccgcctgg
     3361 gctgggttcc aacgacgaat tggtcgcttg acgcaaaata agacagtcgc aagacagaat
     3421 tctgcttgga acccggcgca tttgcccccc atatgggcgg acgcgccgga gcaaccaccg
     3481 gacgcctgga gacagcccgt gagtatccta ttacccgctc ctctggcctg ctgccggtgg
     3541 gagcttgaac ggcgccacat ggtgccacca tccaacgacg ccctgcggca gatcattgag
     3601 cgtgataccg acctggtcga cacgctgaac gaatattacg gagacgccac gcccgacggc
     3661 ggcctcgaca aggtcgaacg cgaactgctg ctggacctgc tcagccgtca ctataccggc
     3721 cagctctggc cgcggaccgg cggcatggaa gccactcgcc ggttcatggc aaacctgcaa
     3781 cgcgccatgg ccgccgctgg ctggaagcta agcggcttcg ccctggccgc ctgactacca
     3841 atcccctctc cgcccgtgga ggcggagagg tagggaaaga tgggggccct ccctccgcgt
     3901 ccccgcttga cgccccgctc cttttgcgcg ctgctgcacc gcaacaaagg gagcgcacgc
     3961 cgtgactcac cctgccctag acgccctgct cgccagcacc gcgcgcccgt tcgaggacgc
     4021 gaccgccatg ccgcccgcgg tctacacatc ctccgacatc ctcgccgtcg agcagcagcg
     4081 catcttccgc cgggaatgga tttgcgtcgg ccgcgccagc gccattgccg aacccggcgc
     4141 ctacctcacc tacgagatcg ccggacagcc catgatcatc ctccgggacg ctgcgtgcgc
     4201 catccgcgct ttctccaacg tctgcctcca ccgctattcg gtcctgttgg aaggcaccgg
     4261 ccgtacgtcg gcaatcatct gcccctacca cgcctggacc tacggcctcg acggcgctct
     4321 ccgcggcgct ccccacatgg accgcacccc cggattctgc cgcgacaact accgcctgcc
     4381 cgagctccgc accgagacct ggcaaggctg gatctacgtc acgctgaatc ctgacatcgc
     4441 ccccgtcgcc gagcgcctgt cgaaactcac cggaatgatc caccagtatc acatggagaa
     4501 ctacatcgaa acgtttcgtg aggaactggt ttgggacacc aactggaaaa tcctcgccga
     4561 aaatttcatg gagagctacc acctccccac cctgcaccgc gctaccatcg gcccggcgtc
     4621 gaagatcgac gaaatggact gccctccagg ctttgacacc ttcaactacc actggatcac
     4681 gaaggacgcc tcgcttccca tcggcaatgc gcatcccgat aacaccaccc tggaaggcga
     4741 ctggcgccgc accgtcgcgc tgatgtcgat ctacccgact cagctgattt ccctcacgcc
     4801 cggctacttc tggtatctgc tcttgtcacc acgcggcacc ggccaagtgc acatcatctt
     4861 cgccggcggc ctcgcacccg agttcatcag cgagcctcgc ggccccgaac acatggctac
     4921 gctcaaagcc ctgctcgaca aggtcaacgc cgaggatcgc agcggcgtcg aaggcgtata
     4981 tcgcaacgca catgcaccac tcgccgctcc gggccacctg agttggctcg agcgtcccaa
     5041 ctacgatttc gcccgctacc tggcgcgcca actcgctgaa gggtgaacct ccatccctcg
     5101 ctccatcgcc ccgaagcgct tgatctcagc tgcaacctcc agtagttgga tcgctcgacg
     5161 aacgacggcc ggacccacct gacccaggtg gggagaaggc gtgacgtctc ggcgccatct
     5221 cccgaaccgg ataccggccg cgttgtgttg cgggagatat tccatggaag aaacggtcgt
     5281 cgcaggtgga cgcatcgctg gcagcagcac cctcacacgc taccaatggc tggtatttct
     5341 cgtcgtctgg ctcggctgga cactcgattc agcggatttc ggcctctacg cgctcgtact
     5401 gcgccctgcg ctgaccgaac tcctcggcgg caatccgcca cttacccagg ttggcagcta
     5461 cggcggcatc ctcagcatga tgggtctgct cggctgggcc atcggcggct tcgtcttcgg
     5521 tatatacgcc gattacgtcg gccgcgtccg cgcgctcgcc accagcatcc tgctctacag
     5581 cgtcttcact gcgcttcagg gcctctccca aggcatctgg gacttcgcca tctatcgctt
     5641 catcgccggc ctcggcaccg gcgccgaact catggtcggc atccctttgc tcgccgaaac
     5701 actcaacagc acgcaccgcg ccaagatcgc cggcgccatg atgacaggcg gcgcgctggg
     5761 cacattcctc ggtgcatggg tctatggata catcggtagc tacggctggc gcgccgtctt
     5821 cttcgtcggt ctgctgcccg ccctgctgct cgccatcata cgccggcgca tgatcgaacc
     5881 cgaccgcttc gccaccgtcc tcgaacgccg ccagaccatc cgcgccggcc agcgcgtcgc
     5941 cgacgacgac agccagttca tgcgcttcgt ccctctgcaa cttttcagcg cggagcatcg
     6001 ccgcaacacg atcgtcggcc tcctgttcgg ccttggttca ctcctggcga tctggacctc
     6061 caacatctgg ctgcccacga tcctgtcgct gatgatccag aaaagcggcg tgaccggctc
     6121 catggcggtc cccttcgtca gccacggcat aatgctctgg agcctcggcg gcatcgccgg
     6181 ttactgcgtg ttcggcttcc tcgccgatct gctcggccgc cgcggcacga tcacgctgta
     6241 cagtctcggc acgatcgccg ccgggctcac cctctacctc ggcctggaca gctattaccc
     6301 ctactaccca atcgttctcc caatcttcgg cttctgtgtg ttcggcacat tctccggctt
     6361 cgccatctac ctgcccgaac tcttcccgac ccacgtccgc tccaccgccg tcggcttcac
     6421 caccggcacc gcgcgtatcg tcaccagctt cggtccgctc gttgccggcc tgatggtcgg
     6481 cgccttcggc ggcagcttca acagggtgac cgccgtcatg acgtgcatgg cgctcttcag
     6541 catcattgcc atggcattcg cacgcgaaac gaaaggcgca gagttgccgc actgaggcag
     6601 ttggaggaac cttggcccct gaaccaccac caggtgtgcc atgaacttcg ccaacaagac
     6661 cgtgatcgtc accggaggaa caggcgcgct cggccgggct gtggttggca cgctgctggc
     6721 cgccggagca cagtgccgcg tgcctttcgt cgtcgatgcc gaggccgcca cgtatccgca
     6781 caaggaccgc gtcaccctga tcggccactg tgagctgacc aacgaggccg acgttgcgcg
     6841 cctctacgac ggtgtcaccg acctctgggc ctcgatccac atcgccggcg tcttcgcgat
     6901 gggcgcagtc gccgacaccg acaagaccgg catgctcgac atgctgaaca ccaacttcgt
     6961 gacctgctac ctgtgcagcc gcgccgccgt gcagaatttc agcgcggccg gcggccgcat
     7021 cgtcaacatc accgcccgcc ccggcatcga accgcgcctg ggcagcggca tggctcccta
     7081 cgccgccggc aaggccgccg tcgccgcctt cacccagtcc ctcgccgccg aggtcgccgc
     7141 cctcagcatc cttgtgaacg ccgtcgcacc atcgatcatc gacacgccag ccaatcgcgc
     7201 ggccatgccc aaagccgacc acgccgcctg gcctaaaccc gaggaaatcg ccgccacgat
     7261 cgccttcctc gcctcacccg acaacaaggt gaccaccggc gcgatcgtcc cagtctccgg
     7321 ccgaacctga cccggcagga gcaaggctcc tgtcattgca agcgaagcga agcaatctcg
     7381 aacgggattg cttcgccacc ccattccccg cgatgccggg atgtccgctc acccctccga
     7441 cagaaactcc atcgccgcgt ccacgccgcc cttaccgtgc ggcacgtgat tgatccggag
     7501 cgacatctcc accgccgaca gcgcccccag cagcatcggt tcgttcagat cccccagatg
     7561 cccaatccgg aacaccctgc ccctcaacgc gcccagcccg ccgcccagcg acacattgaa
     7621 ccgcccccgc gccgtctccc gcagagcatc cgcatcatgc ccctccggca tgacaatcgc
     7681 cgtgactgag tcggagtacc gccccggatt ggtgcagaac aactgcggcc cgttatttcc
     7741 cgcccacacc ttcaccgccc gccgcgtggc ctccgccagc cgcgtatgcc gcgcgaacac
     7801 gttcggcagc ccctcttcct ccagcagccg gatcgactcg ctcagtccgt agaacatgtt
     7861 gaccggcagc gtgccgatga agctcttctg cggccgcttc tccatcagcg tccaatcgaa
     7921 gtagaaccgc ggcagtttcg cgcgcgcatg cgcctccaac cccttcggcg acacgccggt
     7981 gaagctgact cccgtcggca gcatcagccc cttctggctg ccgcccacca cgcagtcgat
     8041 cttccattca tccatccgga aatcgagcga tcccagcgaa gagatcgtgt ccaccaggaa
     8101 cagcgccgga tggttcgccg cgtcgatagc cgcgcggatc tcatccagcg gcagcatcat
     8161 gccggtcgcg gtctcgttgt ggaccacgca caccgccttg atgacagcca acgcatcctc
     8221 ggtcagggtc gccctcagct gcgcgacatc cacgccatgc cgccagtcac agcccaccag
     8281 gcgcacgtcc agcccgtgcg cccgcgccat gttcgcccac tcctcggaga aataccccga
     8341 ctccagcacc aggatcgtat cgcccggcga cagcaggttg gtcagcgagg cctcccacgc
     8401 cccatgcccc gacgcgttgt acatgaacag gtggtgctgc gtttgcagca cccgcttcac
     8461 cccggcgaac gcctcgtcgt acacctcgac gaaatccggg tcatggaagt cgatcgacgg
     8521 ccgatcgagc gcgcgcagca cgctgtccgg aatattggtg ggccccggat tggcgaagaa
     8581 ctggcggcca cggggttttt ggacgcttgt gctcatatgc agaaccttcc gctggggggc
     8641 cgcatccatg gcgcaactca cgggtttgta aaggtccaag ttccgctggc cgccatgcac
     8701 cggaatgccc cgcgccggat atccgtccgg ccaacatcgc cttcgtgagc acggcagccc
     8761 ctgccggcta gcggtgcgcg ttattcttgc tacaccaacg catgaaacgc gccctgctgg
     8821 tccgagaagt cctcgccgac tgctattgcc ggcttcaacc gtccgccata cacggaatcg
     8881 gcgttttcgc tatccgcgac atcccacgcg gcaagaaccc atttcaaatg ctgccccggt
     8941 atgcccgccc cggttacatg aggataaccc acgaggaact tgaagcgctc cctcccggtc
     9001 tcgcccggac gatccgtgca ctcttcgtgc ctaccgacgg cgcgatgtat atacccacct
     9061 gcgggacaaa ccttgtctat ctgattgcat atcttaacca ttccaatgtg cctaacttgt
     9121 gcacccggga cggtttcaac ttcttcactc gccggaaggt tcgcgaagcc gaggaactga
     9181 ctgtcgatta tcgcacctat ggtgcagacg acctgctgcc atcacccaag tccccgctca
     9241 tattgaataa ttaagatctt cttttcgctt atatctacgg gggccaggca gcttgctctc
     9301 gccagcgcaa acaaaaataa tgcttgccgt cacttatact aactaatata acccactgat
     9361 gccagatgac gtcgctgcac acgctccggc cgatcccatc gaacagctcc ccaaggctgt
     9421 ctaccggacc atcattgccg atctgcatgc tcttatcccg ccaccaaccc tcactgaccc
     9481 cgagctcatc gccgagcgcg tccacgccgc catcgcgcag atcgccacca tggtcccggt
     9541 gaacgccgac gaggccaata tcgcgctgcg agtcgtcatc gccgaggcca acgccaagga
     9601 atgcgcccgg catgcccgca tgtacttcaa cgacaccgcc ctcttgctga aatgcgaggc
     9661 ccaggccaac cactgccagc gcaccgccaa tgccgcccgc gccctgctgc ttcgcgtcca
     9721 gtcagcccgc cacaagcgtg aggccaatcg caccacctgt gaccaggacg cctggaccat
     9781 ccatgcgacc cagggctacc tcctcagcgc caacggcgaa cgcgtttctg caccgccgcc
     9841 tccgccaact cctgagccac cttcgccgtc acccaatgcg gccgacgaca ccagcgcccc
     9901 aagtcacacc gaggccgagc tctacgccat catgtaccca cgccgcgcgg cccagatccg
     9961 cgcgcatggt ggcatgcccc ctgacgcccg cttcgaccgc cccgcccctg a
//
LOCUS       DATGEM010000284        13065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1318523, whole genome shotgun sequence.
ACCESSION   DATGEM010000284 DATGEM010000000
VERSION     DATGEM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318523"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VKQ27_08920"
     CDS             <1..910
                     /locus_tag="VKQ27_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY89093.1"
                     /translation="LAEKNGFGYEDLRKVNPRLVYVSISGYGPSGPHADLPGTDSMAQ
                     ALGGVAEAYSTPGQPLRTGIVSVADETCAILAFGGALAALTHARASGVGQKVDCSLLG
                     GQIRLMGWTLTTTMWRNQNPVTGQARVTGTPQSPGLSASFNDRDGKPLVFQLGGRQWK
                     QAMVALGFHEALERAGFADLGVIVASQEQRAILLCTLDDLFATGSRDDWVAKLRGADI
                     VSAPINTLLEASNDPDVVANGYVAEVEYPKHNKTLKVHGTPWRFSETPARIGVAPELG
                     ADIQEVLGELGYTPGEIQGLRDRKII"
     gene            907..1830
                     /locus_tag="VKQ27_08925"
     CDS             907..1830
                     /locus_tag="VKQ27_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY89094.1"
                     /translation="MSATQAALPAPQDIMRGILLMCAGVSTFPFMNAAVKLLAANYPA
                     AQITWARFTGHLLVMLVVFLPQYRSSVIRTRRPAVQIGRSLAMLFSNLLFVMAIGSVP
                     LATASAIGFTSPILVTALSVPLLHESVGWRRWSAVFIGFTGALIVIRPGTGFHDPAVL
                     LILLSSLVYALYQIGTRLVAPYDNAATGIIFAALFGSLGMTLVLPFVWVPPHSVFDTL
                     LFSCLGLLGGLGHYLVILAFQKGPAAVIAPLGYVELIGTTILGYLIFGNFPDFWTWIG
                     AGVIIASGIYIAFRERKRRLSSPAYRTSDST"
     gene            complement(1793..3091)
                     /locus_tag="VKQ27_08930"
     CDS             complement(1793..3091)
                     /locus_tag="VKQ27_08930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY89095.1"
                     /translation="MTSDLFTDDAKLTPYWWDRTPRPALPDATPPAKTDVVVIGSGYT
                     GLSAALQTARGGRETVVLDAEDAGWGCSSRNGGQISTSVKPRYAELVPQYGHETAFRI
                     VKEGQNSLAWIGDFIAAEEIDCDFRLAGRFHAAHNPARYEALAQSLKEQPKGLGIAAH
                     LVPRAEQRRELGTDAYYGGIVYEQHASVDPGRLHQGMLDRALAAGARVVPHCAAMAIE
                     PDGAGLRVTHARGTTTARNVVVATNGYTGSLTPWLRRRVIPIGSYIIATEPLPRETMA
                     SLMPKNRVVSDTRKVVYYYRASPDGQRILFGGRVSHNETNPHISGPRLHADMAAIFPD
                     LANVKISYSWMGFVAYTFDHMMRAGAHEGVHYALGYCGSGVGMAGYLGMRIGQQVLGL
                     KEGATAFDNLPFNTRPLYYGNPWFLAPSIMYYRWCDKRGN"
     gene            complement(3149..3508)
                     /locus_tag="VKQ27_08935"
     CDS             complement(3149..3508)
                     /locus_tag="VKQ27_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HLY89096.1"
                     /translation="MPAEVQITFRGMETSPSVEAQVRRRIEELYQISDRIAACRVVLE
                     AAHRRHRQGMIYHVRIDLTVPGGPLVIDREPGEDHAHEDMRVAVRDAFDAARRRLQDR
                     MRKLGGDVKQRATEAPA"
     gene            3907..4332
                     /locus_tag="VKQ27_08940"
     CDS             3907..4332
                     /locus_tag="VKQ27_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HLY89097.1"
                     /translation="MSVQVLYATRVTASGGRDGRVTTADGSLDLTMSTPTALGGAGAP
                     GNNPEQLFAASYAACFLASMKFVATQGGPQVPENASVCATVGIGPHSQGGFGLDVQLD
                     IALPGASPADADSLVRRAHLICPYSNATRNNVPVRLIIV"
     gene            4610..7459
                     /locus_tag="VKQ27_08945"
     CDS             4610..7459
                     /locus_tag="VKQ27_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY89098.1"
                     /translation="MTGSSAMESASVLGFGPFRLIPSRGLLLEGDKPCRLGSRALLIL
                     TALVERAGETVSKHDLLARAWPDTFVDDANLRVHVAALRKALGDGQAGTRYIMNVMGR
                     GYCFVAPVTRSDSDTQQPAVRGNTQGPSNLPAPLSRVLGRTEAIDSVAVQVPMRRCVS
                     VVGPGGIGKTTFAVAVGERLLAVYDHGVWFIDMASVSERHQIPFAMASVLGVSITSGN
                     PVPSLVGYLRDKHLLLLIDNCEHLVDTIAPLVETILRQAPNVHILATSREALRAEGEW
                     VYRLKPMEAPPPVDSLTAAEALQFDAVQLFVERASASLDGYRLTDADAPAAAEICRRL
                     DGLPLAIELIAARIDLFGVRGLATMLNDHFLLVSEGQRTAQPRQQNLLGALDWSYRLL
                     SSVEQTILCRLAVFQGDFTLDAAVVVATGNGVSQEQVYSGILTLSSKSLITTDVTGAA
                     REHHHRLLHVTRAFVAQKLAQSSDRDRVVRLHADYLCRLFAQAEADWEKTERQVWLAS
                     YARAIDDVRTALDWAFSPQGDAAIGVALTARAIPLGFQLSLIEEVKAWLERALLHAPL
                     LAVPQPLAEMRLNVGLGRLSHNMGLQPGHTTGFDRAIELSQALADPAYRTEPLIALAA
                     FNLTAGKYGAGIALAREAHAIAEASGDAKAVLAVNRIAAQVHHLNGDHVTAQQLATAV
                     LDYPVAQIPLAYNTMPVDRQVSMRVVLARIAWLRGSPQQASALARESVEHAASDSAFS
                     LCQALAMAAIPIALWSGDDAGAAAMTATLDQQASRVSLAFWRSWATAFRALLRVRAGI
                     DMEPPKLSGALQSETFATFSAELLEPVTLQRAENGEAGWCRPELFRAQGEWLLAQGAP
                     GAAKAAEGLFNNALELARQQGALTWELRAAISLTRLSQRRGQSSIGGGLLASVMQQFR
                     DSTATADMVEARELLAQVAPVENRLPQASRRRESA"
     gene            7550..8509
                     /locus_tag="VKQ27_08950"
     CDS             7550..8509
                     /locus_tag="VKQ27_08950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY89099.1"
                     /translation="MEYTTLGRTDIEVSVAGLGCGGFSRLGLSTGGTEASAVAIIHAA
                     IDLGVNLLDTAAPYGTEGVVGKAIKTVPRDKVVIATKSSVFRDGERWTPERVIASLDN
                     SLRELGIDCIDVFQLHAIAPSGYDYVREVIAPALLKEKAKGKFRFLGITETSPNDLEH
                     RMLLRAIPDGLWDTAMVAFHMMSQNTREAVFPATLQHRVGTLLMFAVRSIFARRERVA
                     DAMRELATEGKVPQALADDPDPLGFLVHPGGALNLTDAAYRYVRHEPGVDVVLFGTGD
                     HAHLRSNIASILRPPLPQADRGKLKQLFSHLRGVGLVAPPGAR"
     gene            8506..9258
                     /locus_tag="VKQ27_08955"
     CDS             8506..9258
                     /locus_tag="VKQ27_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89100.1"
                     /translation="MSGHLRLAAGLVFGVVLSVVAHAQDSDALAAQLDRPVSTTRVPA
                     KSDDDPIGQIECTYYRDFMLRETGTDTPDPNDATLVPIAIGAPRPPCDMAESANLLTL
                     KTEGYSYVGRKDHFLLFSATDPNGAVPFMLLDIGDGKVIFEDATLPDRSIQTVVLDNG
                     TLHLGYKRAFNASCSLLQDPASCWSRLVADRKIPNGLAQPSPDLCAVAYKEEQAPVED
                     PSIVSYAVDTTIDASGQVRTTAHGTIGCAPMP"
     gene            9273..10448
                     /locus_tag="VKQ27_08960"
     CDS             9273..10448
                     /locus_tag="VKQ27_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY89101.1"
                     /translation="MKIVRIDDLHCDAGWRTFSFLKISTDDGIAGWSEYTEADGSRGL
                     TGVIRGLAEPLIGTDPRPVQQIASTLWVRQVQAPGGVNARAIAAIENALLDIKGKALG
                     VPVYELFGGPVRERIPVYWSHCGSYRVRNHELVGVPSVRTYDDIAALGAEVKRRGFKA
                     LKTNILPFDGEKLTNFGPGFGRSPGYPELNCDRNTLRDVHDLLSAFRQGAGPDMGLHL
                     DVNYHFKTEGYLQIAKAVAPFDMTWLEIDTWDPQALALIRRAAPCPIASLESVCGRRA
                     FRPFLDNYSCDVAIIDVIWNGFLESIKIAAMAEAYEVNCAPHNYYGHLCSAISAHFCA
                     VVPNFRVMEIDIDSVAWRDELFHNPPAIENGELIIPKGPGWGVDVNEAAVGAHPPRG"
     gene            10561..11544
                     /locus_tag="VKQ27_08965"
     CDS             10561..11544
                     /locus_tag="VKQ27_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HLY89102.1"
                     /translation="MSARFDILGIGNAIVDVVAQSDDSFLSRHDMRKGSMTLIDSDAA
                     DTLYGAMPPGQESSGGSAANTCAVAAALGARVAYLGKVANDQLGNVFRHDMTAIGVHF
                     PSTPLYGGASTARCLILVTPDGQRTMNTYLGACVTFDEADVADALVADAAVTYLEGYL
                     FDPPAAQRAFRRAAAAAHAAGRQVALSLSDAFCVDRHRAAFLELVKQDVDILFANEAE
                     VTSLYEENTFEAAAARARADVALAALTRSEAGSIILRGAETVEITAEPTRVVDTTGAG
                     DAYAAGFLAGLTAGKPLPVCGKLASIAAAEVIGHYGARPQADLKKLAAAAG"
     gene            11659..>13065
                     /locus_tag="VKQ27_08970"
     CDS             11659..>13065
                     /locus_tag="VKQ27_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89103.1"
                     /translation="MRSGPWGAVCAAAIAFVVGIMPATADETPKRGGILTYMIPADAP
                     PSFDGHRETTYATIHATAPFYSVLIRLDPAHPEDVTKFVCDLCTELPTATDNGTSYAF
                     KIRQGVKWHDGSPLTADDVAASWENIIHPPAGYNSARANWYQMVDSISVTGGDTVVFR
                     LNFATLTFLPALADPYAYVYKKEILAKDMHWYEKNIMGSGPFRFAGYELGQSIKGERN
                     ADYYHPPEPYLDGFVGIFADKQVVRVEAIRADRATTEFRGLPPSAIDQLKAGLGDKIT
                     VQESDWNCGNLITPNSKRKPFDDVRVRKALLLAIDQWHGAPPLSKIANVKTVGGIVFP
                     GSPLAATKEELQKMAGFWPDIDKSRAEAKKLLQEAGQQNLSFEMLNRNVDQPYKYVGT
                     WLVDEWSKIGVHVTQKVVPTGPWFEAMRTGNFDVVVEANCNGVVNPVMDTQKYLPKKA
                     FVENYGGYDDLELIDIYGK"
BASE COUNT         2300 a         4174 c         4176 g         2415 t
ORIGIN      
        1 tctggcggaa aagaacggct ttggctatga ggatttgcgc aaggtcaacc cgcgtcttgt
       61 ttacgtatcg atctcgggtt acggcccgag cggtccgcac gccgatctgc ccggcaccga
      121 ttccatggcg caagcactgg gcggagtcgc ggaggcctat tccacgcccg gacagccgtt
      181 gcgcaccggg atcgtatccg tcgcggatga gacctgtgcg atcctggcgt ttggcggggc
      241 ccttgcggct ttgacgcatg cgcgcgcctc gggggttggc cagaaagtgg attgctcgct
      301 gctcggcggc cagatcaggc tgatgggttg gacactcacc accacgatgt ggcgtaacca
      361 gaacccggtg accgggcagg cgcgcgtcac cggtacgccg caaagccccg gcctcagtgc
      421 gagcttcaac gaccgtgatg gcaagccgct ggtgttccag ctgggtggac ggcagtggaa
      481 gcaggcgatg gtcgcccttg gcttccacga agcgctggag cgcgccggtt ttgctgatct
      541 gggcgtgatc gtcgcgtccc aggagcagcg tgcgatcctg ctgtgcacgc tggatgatct
      601 gtttgccacc ggatcacgcg atgactgggt ggcgaagctg cgcggcgcag acatcgtctc
      661 ggctccgatc aacacgctgc tcgaagcttc caatgatcct gacgtggtgg cgaacgggta
      721 tgttgccgag gtggagtacc ccaagcacaa caagacgctg aaagtccatg ggacgccgtg
      781 gcggttctcg gagacgccgg cgcggatcgg tgtcgctcca gaactcggcg ccgacatcca
      841 ggaagtgctg ggtgaactcg gctacacgcc tggggaaatc caaggcttgc gcgaccggaa
      901 gatcatttga gcgccacgca ggcggcgcta ccggcgccgc aggacatcat gcgcggcatc
      961 ctgctgatgt gcgccggcgt ctcgacgttc ccgttcatga acgcggcggt gaagctgctg
     1021 gcagcaaact atccagccgc gcagatcacc tgggcgcgct tcacgggcca tttgctggtg
     1081 atgctcgttg tcttcctgcc gcaatatcgc tcgagtgtga tccgcacacg ccgtcccgcg
     1141 gtgcagatcg gccgctcgct cgccatgttg ttcagcaacc tgctgttcgt catggcgatc
     1201 ggcagcgtcc cgctcgccac cgcctctgcc atcggcttca cctcgccaat cctcgtcacc
     1261 gcgctttccg tgccgctgct gcacgaaagc gtaggctggc ggcggtggag cgcagtattc
     1321 atcggcttca ctggcgcgct catcgttatc cgcccgggca cgggcttcca cgacccggca
     1381 gtgctgctga tcctgttgtc ctctctcgtc tacgcgctct accagattgg cacgcgcttg
     1441 gtcgcgccgt acgacaacgc cgcgaccggg atcatctttg ccgcgctctt cggttcgctc
     1501 ggtatgacac tcgtgctgcc gttcgtctgg gtcccaccgc acagcgtctt cgacacgctg
     1561 ctgttttcct gccttgggct tctgggtggg ctcgggcatt acctggtgat ccttgctttc
     1621 cagaaaggcc cggccgcggt gattgctccg cttggctatg tcgagctgat cggcaccacc
     1681 atcctcggct acctgatctt cggcaacttc ccggatttct ggacgtggat cggcgccggc
     1741 gtgatcattg cgtcgggtat ctacattgcc tttcgcgaga ggaagcggcg cctcagttcc
     1801 cccgcttatc gcaccagcga tagtacatga tcgatggcgc caagaaccat ggattgccgt
     1861 aatagagtgg ccgcgtgttg aacggcagat tgtcgaacgc cgtggcgcct tccttcaacc
     1921 ccagcacctg ctgcccgatg cgcatgccaa gatagcccgc catgccaaca ccggacccgc
     1981 aatagcccag cgcgtaatgc acaccctcat gtgcgccggc gcgcatcatg tggtcgaagg
     2041 tatacgcgac gaagcccatc catgaatagc tgatcttcac gttcgccaga tcggggaaaa
     2101 tcgccgccat atcggcatgc agccttggac cgctgatgtg tgggttggtc tcgttgtgtg
     2161 atacgcggcc gccgaacagg atgcgctgtc catccggtga cgcgcggtag tagtagacca
     2221 ccttgcgggt gtcgctcact acgcggtttt tcggcatcag gcttgccatg gtctcgcgcg
     2281 gcagcggctc ggttgcgatg atatagctgc cgatagggat cacccggcgc cgcagccagg
     2341 gcgtcaggga gccggtatag ccattcgttg caaccaccac attgcgtgcg gttgtggtgc
     2401 cccgcgcatg cgtcacgcgc agtccagcgc catccggctc gatcgccatc gccgcgcaat
     2461 gcggcaccac ccgggcaccg gcagccagcg cacgatcgag catgccctga tgcagccgcc
     2521 ccggatcaac cgaggcatgc tgctcgtaga cgatcccgcc gtaataagcg tcggtgccca
     2581 gttccctgcg ctgctctgcc cgcggcacaa gatgcgccgc gatccccagt cccttcggct
     2641 gttccttcag ggactgcgcc aacgcctcat accgggccgg gttgtgcgcc gcatggaacc
     2701 gccccgcgag ccggaaatcg caatcgatct cctcggcggc gatgaagtcc ccgatccacg
     2761 ccagtgagtt ctgtccctcc ttgacgatgc ggaacgccgt ctcgtggcca tattgcggca
     2821 ccagttccgc atagcggggc ttcacgctgg tcgagatctg cccgccgttg cgcgaactgc
     2881 acccccagcc cgcgtcctcg gcatccagca ccaccgtctc gcgcccgccg cgtgctgtct
     2941 gcaatgcagc gctcagcccg gtgtagccgg agccgatcac caccacgtct gtcttggccg
     3001 gcggagtggc atcgggcagc gctgggcgcg gcgtccgatc ccaccagtac ggcgtcagtt
     3061 tcgcgtcgtc ggtgaacagg tcgctggtca tagcttcggt ctcgttcggc tcgtcgtgtc
     3121 gaacgttacc gtcgggtggc ggcgtcggct atgcaggggc ctcggtcgcg cgctgcttca
     3181 cgtcaccgcc gagcttccgc atccggtcct gcaaccttcg gcgcgctgca tcgaacgcgt
     3241 cccgcacagc cacgcgcatg tcttcatgcg cgtgatcctc gcccggctca cggtcgatca
     3301 ccagtggccc gcctggaacg gttagatcga tgcggacgtg gtagatcatg ccctgccgat
     3361 gacgccgatg cgcagcctcc agcacgaccc ggcacgcggc gatgcgatca gagatctggt
     3421 acaactcctc gatgcggcgc cgcacctgcg cttcgaccga tggcgaagtc tccatgccac
     3481 ggaatgtgat ctggacttct gcgggcattc cagcctcctt cttcgacgcg gaaagcttaa
     3541 cgtcccgccg ctcattggcc ttgatctcga tcaaacgacg caacgatagc gcgcggcatc
     3601 caaaggagga gcgcgaccgg ctgccaccgc gctggcacga tgatggtttt cggcaaatcc
     3661 gtgtcgattc ttccaagcgg cgaagcagaa tggccggcaa gattcatgcg tgctcaacgg
     3721 ggacgggtga tgatgaattc cggcgcggcg accatcctca ccgctcatgg cgtgcagatt
     3781 tcagccagac ggacggggcg cgggacgacg agctgttcgg gcgcggcgcg attacgcgcc
     3841 ttgttggtat tggaagccgc acactagccg cggctgactt cccccaatca accgggacga
     3901 ccgcacatgt cggtgcaggt gctttatgca acgcgggtca ccgcgtcggg cggccgcgac
     3961 ggccgcgtga cgactgcgga tggcagcctc gatcttacga tgagtacgcc aacggcgctg
     4021 ggtggcgccg gggcgccggg caacaatccg gaacagctct tcgccgccag ctatgctgcg
     4081 tgcttcctgg catcgatgaa attcgtcgcc acccaggggg gaccacaggt gcctgagaac
     4141 gcgtcggttt gcgccaccgt cggcatcggt ccgcactcac agggcggatt tggcctcgat
     4201 gtgcagcttg atatcgcgtt gccgggcgct tcgcctgccg atgcagactc actggtgcgg
     4261 agggcccacc tcatctgccc ctattccaac gcgacacgca acaacgttcc ggtacggctg
     4321 attatcgtat gactgtggaa cgacccccgc cggggccggt cgacgccgcg taaagcggtg
     4381 ttaattcctg taaagcgtgg gtcggccagc gttacgcgac aagtgccatt tttgcccgac
     4441 tgcctgggat aatggcgggc attgttgcgt gcccccggac ggattgacgg tacatcagaa
     4501 ctcttcagca attgcccaag gaaactccac ggcaggtcag gctagtacaa actatcgggc
     4561 cagcctaatg agcccggcaa tgaccgccct gtgggaggct cgggtcaaaa tgacgggctc
     4621 atcagcaatg gagagcgcat cggttctggg atttggcccg ttcaggctaa tcccgtcgcg
     4681 cgggctgctg ctggagggcg acaagccgtg ccggctcggc agccgggcgc ttctcatact
     4741 gaccgccctg gtggaacggg ctggcgagac cgtcagcaaa cacgacctac tcgcccgcgc
     4801 ctggcccgac acgttcgtcg acgacgccaa tctgcgcgtg catgtcgccg cgctgcgcaa
     4861 ggcactcggc gacggccaag caggcacccg ctacatcatg aacgtcatgg gtcgcggcta
     4921 ttgcttcgtg gccccggtta cccgctccga cagcgataca cagcagccag ccgtccgggg
     4981 gaacacgcag gggccgagca atctccccgc gccattatcg cgcgtgttgg gccgtaccga
     5041 ggcgatcgac tcggtcgcgg tgcaggtgcc catgcggcgc tgcgtgagcg tcgtagggcc
     5101 gggcggcatc ggcaagacca ccttcgcggt tgctgtcggc gaacgtctgc tggcggtcta
     5161 tgatcacggc gtctggttca tcgacatggc ctctgtctct gaacggcatc agattccgtt
     5221 cgccatggcg tcggtgctgg gagtttcgat cacctccggc aatccggttc ctagcctcgt
     5281 tggttacctg cgcgacaagc acctgctgct gctgatcgac aattgcgagc atctggtcga
     5341 tacgatcgct ccgctggtgg aaaccatcct gcgccaggca ccgaacgtcc acatccttgc
     5401 gacgagccgc gaagccctac gggccgaggg tgagtgggtg taccggctga agccgatgga
     5461 ggcgccgccg cctgtcgact cgctgactgc cgccgaggca ctgcaattcg atgccgtgca
     5521 gttgttcgtc gagcgcgcgt ccgccagcct ggacgggtat cggctgaccg atgcggatgc
     5581 gcccgccgcg gcggaaatct gtcgccggct ggacggcctg ccactcgcca tcgagttgat
     5641 cgcagcccgc atcgacctgt tcggcgtgcg cgggcttgcg accatgctga acgaccactt
     5701 cctgctggtg tcggaaggcc agcggaccgc ccagccgcgc cagcagaacc tgctgggtgc
     5761 gctggactgg agctaccggc tgctgtcgtc ggtcgagcag acaatcctgt gccggcttgc
     5821 ggtgttccaa ggcgatttca cactggacgc cgcggtggtg gtagccaccg gcaacggtgt
     5881 cagccaggaa caggtctatt cggggatcct cacgctttcc agcaaatcgc tgatcacgac
     5941 cgacgtgacc ggtgcggcgc gcgagcatca tcaccggctg ttgcatgtca ctcgagcctt
     6001 cgttgcgcag aagctggcgc aatccagcga ccgcgaccgc gtcgtgcggt tgcacgcgga
     6061 ctatctctgc cgtttgttcg cccaggctga agctgactgg gagaagactg aacggcaggt
     6121 ttggcttgcc agctatgcgc gggcgatcga cgacgtgcgc accgcgctcg actgggcgtt
     6181 ttcaccgcag ggtgatgcag ccattggcgt ggcattgacg gcgcgtgcca ttccgcttgg
     6241 cttccagctt tcgctgatcg aagaggtaaa ggcgtggctc gaacgggcgc tgttgcacgc
     6301 gcccctcctg gctgtcccac agccactggc ggagatgcgc ctgaacgtcg ggctggggcg
     6361 cctttcgcac aacatgggac tgcaaccggg gcacaccacg ggcttcgatc gtgccatcga
     6421 actgtcgcag gcgcttgccg atcccgccta tcgcaccgag ccgctgatcg cgctggccgc
     6481 gttcaacctg accgcgggga agtatggtgc cggcatcgcg ctggcgcgcg aggcacacgc
     6541 gatcgcagag gcatcgggcg acgccaaggc ggtgctggcg gtcaatcgca tcgccgcaca
     6601 ggtgcatcat cttaatggcg atcatgtgac ggcacagcag ctcgcgacgg ccgtgctgga
     6661 ctatcctgtc gcgcaaattc cgctcgccta taacaccatg ccggtggatc gccaggtgtc
     6721 gatgcgtgtc gtgttggcgc ggattgcgtg gttgcgtgga tctccacagc aagcgagtgc
     6781 ccttgccagg gaaagcgtcg agcacgcagc tagcgacagt gcgttctcac tgtgccaggc
     6841 gctggcgatg gcagcgattc cgatcgcgct ttggagtggt gatgacgccg gcgcggcggc
     6901 gatgaccgcc acgctcgatc agcaggccag ccgcgtttca ttggccttct ggcgcagttg
     6961 ggctacggcc tttcgcgcgc tgctgcgcgt gcgcgccggt attgatatgg aaccgccgaa
     7021 actctcgggt gcgctgcaat cagagacgtt cgccacattc tcagcggaac tgctcgaacc
     7081 ggtgacgctc cagcgggctg agaatggtga ggccggctgg tgccgtccgg aactgttccg
     7141 cgcgcagggc gaatggttgc tggcccaggg cgcgccagga gcggccaagg cggccgaggg
     7201 gctgttcaac aacgcgcttg agctggcccg tcagcaaggc gcgctcacgt gggaacttcg
     7261 ggctgccatc agcctgaccc ggctttcaca gcgtcgcgga cagtcgagta tagggggtgg
     7321 gttgcttgca agcgtgatgc agcagtttcg cgacagtacc gcaaccgccg acatggtgga
     7381 ggcgagggaa ctgctggcgc aggtggcacc tgtcgagaac agactgccgc aggcgagccg
     7441 gaggcgggaa agcgcctgac gcgtccggta ttcgcggcaa cgccggctca actggcaaca
     7501 catcctgccg cgcgctagcc tctgtcttat cgggggagca ggtgcgcgca tggaatacac
     7561 gacgctcgga cggacggaca tcgaggtaag cgtcgcaggt ctgggctgtg gcggcttcag
     7621 ccggcttggc ctcagcaccg gcggcaccga ggcgagcgcc gtcgccatca tccatgcagc
     7681 gatcgacctg ggcgtgaacc tgctcgatac cgccgcgccc tatggcaccg agggcgttgt
     7741 cggcaaggcg atcaagaccg tgccgcgcga caaggtggtg attgccacca agtcatcggt
     7801 attccgcgat ggcgaacgct ggacgccgga gcgtgttatc gccagcctcg acaactcgct
     7861 gcgcgagctt ggcatcgact gcatcgatgt gttccagctc cacgcgatcg cgccatccgg
     7921 ctacgattat gtgcgcgagg tgattgcgcc ggcgctgctg aaggagaagg ctaagggcaa
     7981 gttccgcttc cttggtatca cggaaacgtc gccgaacgat ctggaacacc ggatgctgtt
     8041 gcgcgccatc cccgacgggc tttgggatac ggcgatggta gctttccata tgatgagcca
     8101 gaacacgcgg gaggcggtgt tcccggccac gctgcagcac cgcgtgggca cgctgctgat
     8161 gttcgcggtg cgcagcatct tcgcccgccg tgaacgcgtg gccgacgcga tgagggaact
     8221 ggctaccgag ggcaaggtgc cgcaagcgtt ggctgacgat cctgatccgc tcgggttcct
     8281 ggtgcatccg gggggagcgc tcaatctgac cgacgcggcg taccgctatg tgcggcatga
     8341 gccaggtgtc gatgtcgtgc tgttcggcac cggtgatcat gctcacctgc gttcgaatat
     8401 cgcgtcgatc ctgcggccgc ccttgccgca ggcggatcgc ggcaagctga agcagctgtt
     8461 cagccatctg cgcggcgttg gtctcgtcgc accgccgggc gccaggtgag cgggcacctt
     8521 cgcctcgccg ctgggctggt gttcggcgtg gtgttgtccg ttgtggcgca cgcgcaagac
     8581 tccgacgccc tcgccgccca gcttgatcga cctgtatcta caacgagggt gccggcgaaa
     8641 tcggacgatg atccgattgg ccagatagaa tgcacgtatt atcgcgactt catgctgaga
     8701 gagactggca ccgacacgcc cgatccgaat gacgcgacgt tggtcccgat cgcgataggt
     8761 gcaccacgtc cgccctgcga tatggccgaa tccgccaacc tgctcaccct gaaaaccgag
     8821 ggatacagct acgtgggccg caaggatcac ttcctgctgt tcagcgccac agatcccaac
     8881 ggcgctgtgc ccttcatgct gctcgacatc ggcgacggga aggtgatctt cgaggacgcc
     8941 acgctacccg atcgcagcat ccagaccgtc gtgctggaca atggcacgct ccatctgggc
     9001 tacaagcgcg cattcaatgc gtcatgctcg ctgctgcagg atccagcgag ctgctggtcg
     9061 cgcttggtcg ccgatcggaa aataccgaac ggcctggctc agccatcgcc ggatctgtgt
     9121 gccgtcgcct acaaggagga gcaggcgccc gtcgaagatc ccagcattgt cagttatgcc
     9181 gttgatacta cgatagatgc ttccggccag gtacgcacga cggcgcacgg caccatcggc
     9241 tgtgcgccca tgccatagtt gggagtaagt ccatgaagat agtccgtatc gacgacctgc
     9301 actgcgatgc tggatggcgc acgttctcgt tcctgaagat cagtacggac gacggcatcg
     9361 ccggctggtc cgagtatacc gaggccgacg gcagccgcgg tcttaccggc gtgatccgcg
     9421 gccttgccga accgctcatc gggacggacc cgcggccggt gcagcagatc gcctcgacgc
     9481 tgtgggtgcg ccaggtgcag gcgccaggtg gcgtcaatgc ccgcgccata gccgcgatcg
     9541 agaacgcgct gctggacatc aagggcaagg cgctcggcgt gccggtctat gaacttttcg
     9601 gtggtccggt gcgcgaacgc atcccggtct actggtcgca ctgcggctcc taccgagtgc
     9661 gcaaccacga acttgtcggc gtgccgtcag tgcgcaccta cgacgacatc gcagcactcg
     9721 gtgctgaagt gaagcgacgt ggattcaagg cgctgaagac caacatcctg ccgtttgatg
     9781 gcgagaagct gaccaacttt ggtccaggct ttggccgctc ccccggctat cccgagttga
     9841 actgcgaccg caacacgttg cgcgacgtac atgatctgct gagcgcgttc cgtcagggtg
     9901 ccggccctga catgggcctg catctcgacg tcaactacca cttcaagacg gagggctatc
     9961 tgcagatcgc caaggctgtc gcaccgttcg acatgacctg gctggagatc gacacctggg
    10021 atccgcaggc gcttgcgctg atccgccgcg ccgcgccgtg cccgatagca tcgctggaat
    10081 cagtgtgcgg gaggcgcgcg ttccgaccct tcctggacaa ctactcgtgc gacgtggcga
    10141 tcatcgacgt gatctggaac ggcttcctcg aatcgatcaa gattgcggcg atggcagagg
    10201 cctatgaagt gaactgcgcg ccgcacaatt actacgggca tctttgttcg gcgatcagtg
    10261 cgcatttctg tgcggtcgtg cccaacttcc gggtgatgga aatcgacatc gacagcgtgg
    10321 cctggcgcga cgaactgttc cataatccgc cggcgatcga gaacggcgaa ctgatcattc
    10381 cgaagggccc cggctggggt gtcgacgtca acgaagccgc tgttggcgcc catccgccac
    10441 gtgggtaacg aggcttgaac cacagatgga cacagatagt tccagcagcg ccgtgatccg
    10501 tgtccatctg tgttaatctg tggttcaaaa tacaattcct caatacaacg aaagtccgtc
    10561 atgagcgcac gcttcgacat tctcggcatc ggcaacgcga tcgtagacgt ggtcgcgcag
    10621 tcggacgact ctttcctgtc gcgccacgac atgcgaaagg gctcgatgac gctgatcgac
    10681 tcggacgccg ccgacacgct gtacggtgcg atgccgcccg gccaggaaag cagcggtggg
    10741 tcggcggcca acacctgtgc tgttgcggcg gcactcggtg cgcgcgtcgc ctatctcggc
    10801 aaggtcgcca acgatcagtt gggcaatgtg ttccgccatg acatgaccgc catcggcgtg
    10861 cacttcccgt ccacgccgct gtacggcggc gcttccacgg cgagatgcct gatcctggtc
    10921 acgccagacg gccagcgcac catgaacacc tatctcggtg cctgcgtcac cttcgatgag
    10981 gctgatgtcg ctgatgcgct cgtcgctgat gcagccgtca cttacctgga aggttacctg
    11041 tttgatcctc ctgccgcgca gcgtgccttc cgccgtgctg ccgctgccgc acatgccgcc
    11101 ggccgacagg tcgcgctgtc gctgtcggat gcgttctgcg tcgatcggca tcgcgccgca
    11161 tttctcgaac tggtgaagca ggacgtggac atcctgttcg ccaacgaagc cgaggtgacc
    11221 agcctgtatg aagagaacac gttcgaagcc gccgcggcac gcgcccgtgc cgatgtggcg
    11281 ctcgcggcgc tcacccgcag cgaggcaggc agcatcatcc tgcgcggcgc cgaaacggta
    11341 gagatcacag cggaaccgac ccgcgttgtg gatacgacgg gcgccggcga tgcctacgcc
    11401 gccgggtttc tcgcagggct tacggccggc aagccgctac cggtgtgcgg caaactcgcc
    11461 tcgatcgcgg ctgctgaggt gatcggccac tacggggcgc ggccgcaggc cgatctgaag
    11521 aagttggcag ccgccgccgg gtagcgggct cgcggaggct cgacggcaag gcttttttcg
    11581 ggcagtacgc caagcgtaac catcggcacg ccccgcgggg aattgccaag aatcaacaat
    11641 gcccgggagg catcgatcat gcgttcagga ccgtggggcg cggtttgcgc cgccgcaatc
    11701 gcattcgtcg tcggcatcat gcccgccact gccgacgaga cgccaaagcg cggtggcatc
    11761 ctcacctata tgatccccgc ggatgcgcca cccagtttcg acggccatcg cgaaaccacg
    11821 tacgcgacga tccacgccac ggcgccgttc tacagcgtgc tgatcaggct tgatcctgcc
    11881 catcctgagg acgtgacgaa attcgtttgc gacctctgca ccgagttgcc gacggccacc
    11941 gacaacggca ccagctatgc cttcaagatc cgccagggcg tgaagtggca cgacggctcg
    12001 ccgctcaccg ccgatgacgt cgcggcaagc tgggagaaca ttatccaccc gcccgcgggc
    12061 tacaacagcg cgcgcgccaa ctggtaccag atggtggaca gcatctcggt caccggcggc
    12121 gacaccgtcg tgttccgctt gaatttcgcc acgctcactt ttctcccggc gctggctgat
    12181 ccatacgcct atgtatacaa gaaggaaatt ctggcgaagg atatgcactg gtacgagaag
    12241 aacatcatgg gcagtgggcc attccggttc gccggctatg agctaggcca gtcgatcaag
    12301 ggcgagcgca acgcggacta ctaccaccca cccgagccct acctggacgg ctttgtcggc
    12361 atcttcgccg acaagcaggt ggtgcgcgtg gaggcgatcc gcgccgatcg cgcgaccacc
    12421 gagttccgcg gtctgccacc atcggcaatc gatcagctta aggcggggct cggcgacaag
    12481 atcaccgtgc aggagagcga ctggaattgc ggcaacctga ttacgcccaa ttccaagcgg
    12541 aagccgttcg acgacgtgcg ggtgcgcaag gcgctgctgt tggcgatcga ccagtggcac
    12601 ggcgcgccgc cgctgtcgaa gatcgccaac gtgaaaaccg tcggcggcat tgtcttcccc
    12661 gggtcaccgc tggccgcgac caaggaagag ctgcagaaga tggccggctt ctggccggac
    12721 atcgacaaat cgcgcgcgga ggcgaagaag ctgctgcagg aggcggggca gcagaacctc
    12781 agcttcgaga tgctgaaccg caacgtcgat caaccataca aatacgtcgg cacctggctg
    12841 gtcgatgagt ggagcaagat cggcgtgcat gtgacgcaga aggtcgtgcc taccggccca
    12901 tggttcgaag cgatgcgcac cggcaacttc gacgtcgtgg tggaggccaa ctgcaatggc
    12961 gtggtcaacc cggtgatgga cacgcagaag tacctgccga agaaggcatt cgtggagaac
    13021 tacggcggct atgacgacct ggaactgatc gatatctacg ggaag
//
LOCUS       DATGEM010000285        13792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_993666, whole genome shotgun sequence.
ACCESSION   DATGEM010000285 DATGEM010000000
VERSION     DATGEM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993666"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKQ27_08975"
     CDS             complement(<1..644)
                     /locus_tag="VKQ27_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HLY89104.1"
                     /translation="MGPLLEVEGLTRHFSVRQGLVIGKTIGVVRAVDGISFSLDRGET
                     LALVGESGCGKSTTARCVLRLIDPTGGNIRFEGTDITEMRGEPLRKLRRRMQIVFQDP
                     FASLNPRMTVGEILEEPLIVHAIGDRMARRARVNQLLGLVGLASYHTQRYPHEFSGGQ
                     RQRIGIARALAVEPSLVVCDEPVSALDVSIQAQVVNLLKELQARLGLSYLFIAH"
     gene            complement(665..1648)
                     /locus_tag="VKQ27_08980"
     CDS             complement(665..1648)
                     /locus_tag="VKQ27_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89105.1"
                     /translation="MSALLSVNGLRTVFHTHNGEVAAVDGVDLSVERGRTLGIVGESG
                     CGKSVLSLSVMRLVPSPGRIASGEILFEGKNLLDLSPAEIRGIRGNRIAMIFQEPMTS
                     LNPVFTVGDQITEAMRAHDRSASSAALRSRAISALQRVRIPAPERRFDEYPHQMSGGM
                     RQRVMIAMALACAPDLLIADEPTTALDVTVQAQILDLLRELQQQTGMAIILITHDLGV
                     VAEMANEVAVMYAGRVVERASGTAIFDDPQHPYTLGLLGSIPKIEENHDRLLAIEGTV
                     PAPFALPAGCRFHPRCVFSDAACAMQDPELRLLAEDHRAACIRAPVESLVS"
     gene            complement(1645..3075)
                     /locus_tag="VKQ27_08985"
     CDS             complement(1645..3075)
                     /locus_tag="VKQ27_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY89106.1"
                     /translation="MITVIRNADMVVAWDAATKSHVYMPDADVAFEGGVLRFVGRGYD
                     GPADEAVSGSGMMVMPGLVNIHSHPSSEPMNKGLIDEIGSPGFYNSSLYEYLPIFRAD
                     AEAIPHCVRVALSELLLSGVTTLADLSMAHPGWLDLLAEAGMRVCVAPMFRSGRWYTK
                     DGHLVEYAWDEAAGEKAMGEALSLIERAEQHPSGRLFGMVVPAQIDTCTEGLLRESFA
                     EAKARGVSWQIHAAQSVSEFHEITRRHGYTPIGWLDHLGLLSERSIIGHGIFLDDHPS
                     TRWHTDSDVKRLAETGTTVAHCPTVFARRGITLKDFGRYRRAGVNMGVGTDTYPHNML
                     DELRLVSYLARTQAGNPRTMNSADLFEAATIGGARALGRDDIGRLSPGCRADIVLVDI
                     THPMMRPSHDPIRSLIYAAGDRAVRTVYVDGQKVVDDGRVLTMDYPAAAAALNDAQKR
                     IKGNVPNLDWDHRTAEQISPLTFRMQ"
     gene            complement(3072..3767)
                     /locus_tag="VKQ27_08990"
     CDS             complement(3072..3767)
                     /locus_tag="VKQ27_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HLY89107.1"
                     /translation="MADRPFTQADLDVLAQWDTPTICNGLEITSPERRAFGYTVEPMV
                     TMYPKAKPIVGLARVGMIRSTEPPRGKVTDRADWYEYVAAKDFPTIAVIQDIDGRIGY
                     GAYWGEVQSNIHKALGSIGCVTNGSFRDMDMWAEGYQMIGGRVGPSHAWVHMVDFGKA
                     VNIFGMQVGHDDVIHADYHGAVVVPADAVKKLPAAIDLVSRREKVILDFCHSPDFSAA
                     KLREILKRSAEIH"
     gene            3806..4573
                     /locus_tag="VKQ27_08995"
     CDS             3806..4573
                     /locus_tag="VKQ27_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLY89108.1"
                     /translation="MQIIAKGGKALYGAPLGILMLEARFPRIPGDMGNAATWPFPVLY
                     RVVKGATPERVVLNAAAGLLDDFLAAAAELVDQGAEALTTNCGFLSLFQAELAAHVKV
                     PVATSSLIQVPWVQATLPPGKRVGIITVSGGSLTPRHLAAAGVPPDTPFVGTEDGREF
                     FRVLVLGEKQDMDVALAAADILQAGRTLLSRHPDIGAIVLECTNMPPYAFALRETLGL
                     PVYDIYSLISWFHTGLRPREFGPPADRATCLPDVPRP"
     gene            complement(4550..5554)
                     /locus_tag="VKQ27_09000"
     CDS             complement(4550..5554)
                     /locus_tag="VKQ27_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLY89109.1"
                     /translation="MSARHLLRSLAYHTGALSLARLRQRDTLTVLMLHRVLGRADPDF
                     ASADPAWTLSLSLFEQLLDFVVRHYSVVSLGDVIGAQEGLRPLPQRALLITFDDGWAD
                     NLRYAAPALRRKGLPAVVFAVPAAIASPGQCWWQELVFAATRLRKLDGWLARDEVRHA
                     VAALGDEPNGLEIVSRLGVMEEAARERLLTGIPASPCHARMMLEPGELQQLIEHGVAI
                     GLHGYSHVPLTRVPDVTAELRLAREAVEVLSGGKATISALGCPHGLYDNKVIDAARVQ
                     GIKHIFTSDPHLNATRGGMLDRTRTIGRINVVGRHIELARDELDPAGAARWLWARDVR
                     "
     gene            complement(5551..7587)
                     /locus_tag="VKQ27_09005"
     CDS             complement(5551..7587)
                     /locus_tag="VKQ27_09005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89110.1"
                     /translation="MTAPLVTVLIPAYNAAATIARAINSVLAQTFSDYEIIVVDDGSQ
                     DTTSEIVAAYRDERIRLFRLPQNRGESGAMNEGIAAARGELVAFLDADDEWLPTKLAK
                     QVTVLRNSPDAVIVTCGCRFIDGQGNVFREFGILPPGIKPTDVWRSLLSATFIAKPCV
                     VARLSALRSVGPFDTKLAIAADQDMWIRLAIIGAVEFVQEFLTTVHDTAGSLTKVYAH
                     KVDTYVLPMIQRHLAEQRSRLSDSEIRGILGDRFTSVGRNLYLTGSILRGAALIGRAV
                     MLGDQIPENLWYLAAASPPARILKKLVGHPGGRPQVSENPRVRTLSDDGLLRPSATDV
                     ADIPPGPLVLIVLIDAEAEFDWDGPFLRSLVSVRNLSRQVSAQNIFDRLKVRPTYLVD
                     FAVATQPEGYQPIRELLQSGRCEVGAHLQPWENPPFVEELSVRTSFNHNLPAWLQKEK
                     LRQLTDVIGSSFGIRPTSYRAGRYGVGDEMAWILEDLGYQIDVSVLPGYDLRSRHGPD
                     FRHAFTRPYWFGRQRKLLEIPLTTGFSGLLARGSEPQVSSATLYAALSSHGAAKLHMP
                     GIFARLGLLERITLTPEGMSIQELKHLTKLLLRRGHRVFTFNYHSSALLPGFTPYVRT
                     QADLDRMLQTIEEYLRFFIDQLGGVAMTPSEFRTSVLARATGAPVPELVGSLTQ"
     gene            complement(7594..8676)
                     /locus_tag="VKQ27_09010"
     CDS             complement(7594..8676)
                     /locus_tag="VKQ27_09010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89111.1"
                     /translation="MDSSSIRADVMRPSALGASEISLWQEMMKARRSLQRAFFSPAFA
                     LACERATDRAYVSVVYDGDGIKAFLPFQFATAWHQRLRLAERIGGGISDAAGLIARVG
                     FTADAGALMRVSGLASMFVTHLVEDQGQFGLDAAWCDTGYVTDVGADSDSYFAAVGER
                     SRDFVRDTERRLRRAERDLGRLEVVRFDPVPGALIAEIIAQKRAQYKRTEVADPFSAG
                     DSMRVISAMNETAELDCRLVLTRLEANSRVLAQHLGPQCQDVLNYWFPVYDPEVRNIS
                     PGRLLLWQTIRRAPQDGIKLIDRGVGDAPYKREFATGTTRYGRGNWYAGTMRSMAARS
                     WLSMEWRLRAWRRRVIARRRQNIDAQ"
     gene            complement(8717..10300)
                     /locus_tag="VKQ27_09015"
     CDS             complement(8717..10300)
                     /locus_tag="VKQ27_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89112.1"
                     /translation="MKRRDVLKSAVAGAAALAAPRVVHAEGAKTINFTPHADLASLDP
                     VWTTADITRNFSLAVFDTLYGYDAGFTVQPQMVEGHVISNDGKQWDLTLRDGLGFHDG
                     TPVLARDCVATIQRFAKRDPFGGALMARTDEISAPTDKVIRFKLKQPFALLPNALAQV
                     YCAIMPERLAKTDAMQQVAEAVGSGPFKFVASERVPGQRVVYVKNDKYVPRKDGVPSF
                     NAGPKIVYIDRVVWNFIPDPATAAAALAQKEIDWWENPTIDLVPQLRRDKDLTLAVKD
                     RTGEIGCLRFNHLFPPFDNPAIRRVVVDAMDQKEIMEAVAGADPSLITTDVGIFVPGT
                     PMASKLGVEITRGPKNIDKLKKDLVAAGYKGEKIVILAASTIPTIWAEAQVASDTLKK
                     IGFNTDFQSLEWGTVVQRRASKEPIDKGGWNIFYTYLGGFGNITPAAAIPIQSSGTNG
                     SWFGWPTDPKMEELRTAWFNAPDVAAQKKICDEMQAEFWQSPPYAPLGMYVQPTAFHN
                     YLTDVRDGWPQFYGVKKNA"
     gene            complement(10349..11197)
                     /locus_tag="VKQ27_09020"
     CDS             complement(10349..11197)
                     /locus_tag="VKQ27_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89113.1"
                     /translation="MTDLSALVAGPGGHSIRYQDPAFPDRPLTLHAARPRDWSREMPV
                     LFVHHGVGRNGRDYRDYWLRLVDEIQVFVIAIEFSEAFFPDYLWYHFGNRHDEDGTLN
                     PSEQWTYGVDERLFAALRAQGVTSQQRYGLFGHSAGGQYVHRMLSFGYRDHVAVAVSA
                     NAGTYAMPDLDVAWPFGLGETELDPPALGRLLEFPITVMTGTGDIKTTGRYFPKGPRS
                     MHQGATRHERAHNYVRAGHAAAEALGTRCAWTVINVPDVGHDGKRMSAAAAPVVARAL
                     HAENNC"
     gene            11294..11920
                     /locus_tag="VKQ27_09025"
     CDS             11294..11920
                     /locus_tag="VKQ27_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLY89114.1"
                     /translation="MQLAIIGAGNVGSALAAGWSAAGHRITFGVREPQAAPSGGATGT
                     TYASVAAAAKSAEVLILSVPWQAVPEALASAGDLTGKILIDCTNPLQMGPDGLELAIG
                     QTSSGAERVAELAPGASVFKTLNQTGFANMEHAHEFALPAVMYVAGDDEMRKPLVMSL
                     VAELGFQAVDAGRLRIARLLEPMAMLWIHMALNRGEPLTTAFALSRRP"
     gene            complement(11921..12157)
                     /locus_tag="VKQ27_09030"
     CDS             complement(11921..12157)
                     /locus_tag="VKQ27_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89115.1"
                     /translation="MHGLTEPVRGIGDNGGPPLDPGIDPNESWRHWCWKRAVKRAWKT
                     PGREIAMRRLTRAEALGISYREYTLEILERGVYL"
     gene            complement(12141..12935)
                     /locus_tag="VKQ27_09035"
     CDS             complement(12141..12935)
                     /locus_tag="VKQ27_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HLY89116.1"
                     /translation="MYDFTYQKPSSLADAVKALVEDGDAKALAGGMTLIPVLKQRLNK
                     PSLLVDLAKLGLVGVSVSGNTVTIQGMTTHGAVAQAAGLKGKIPSLAELASHIGDEAV
                     RHRGTIGGSIANNDPAADYPAAALALGATIKTNKRSIKADDFFQGMFTTALEQGELIT
                     AVDFPVPEKGAYEKFRNPASRYAIVGVYVAKTAGGVRVAVTGAGQSGVFRHTAMEQAL
                     TANFSPDAIAGITTPADGLNSDIHASAEYRAHLVGVMAKRAVARAY"
     gene            complement(12956..>13792)
                     /locus_tag="VKQ27_09040"
     CDS             complement(12956..>13792)
                     /locus_tag="VKQ27_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY89117.1"
                     /translation="QGHETTMAQLVVDQLGVPLDQVEVVHGDTGKIPFGMGTYGSRSL
                     AVGGSAMVKAMDKIVAKGKKIAAHLMEASVEDIEFKDGKFSVAGTDKEKTLTEISLAA
                     YVPHNYPIEELEPGLDETAFYDPKNFTFPGGCHVAEVEVDKETGTVEVVNFVAVDDVG
                     RVINPMIVEGQVQGGVAQGIGQALLENAVYDGAGQLLSGSMMDYTMPRADDLPNITVG
                     TEITLCTHNPLGSKGCGEVGAIGSPPAIVNAIVDAMRDYNVRHINMPVTPQKLWSLMA
                     AK"
BASE COUNT         2686 a         4684 c         3934 g         2488 t
ORIGIN      
        1 tcgtgcgcga tgaacaaata agacaggcca agccgcgcct gcagttcctt cagcaggttc
       61 accacctgcg cctggatcga gacatccagc gccgagaccg gctcgtcgca gacgacgagc
      121 gacggctcca cggccagtgc ccgagcaatg ccgatacgct ggcgctgccc gccggaaaac
      181 tcgtgcggat atcgttgcgt gtgatacgat gcgagaccca ccaggccaag caattggttc
      241 acccgcgctc gccgcgccat gcggtcgccg atggcgtgca cgatgagtgg ctcctccagt
      301 atttcgccca ccgtcatgcg cggattcaac gacgcgaacg gatcctggaa cacgatctgc
      361 atgcgacggc gcagttttcg cagcggctcg ccgcgcatct cggttatgtc ggtgccttca
      421 aaacggatgt tgccaccggt cggatcgatc agccgtagca cgcagcgggc ggtggtggac
      481 ttgccgcagc ccgactcacc caccagcgcc agcgtctccc ctcggtccag actgaaggaa
      541 attccgtcga cggcgcgcac cacaccaatg gttttgccga tcaccaggcc ttggcggacg
      601 ctgaaatgcc gcgtcaggcc ctcgacttcc agaagcggcc ccatcaccgg acttgatccc
      661 gccatcacga aaccaggctc tcgacgggcg cgcggatgca ggcggcgcga tggtcctcgg
      721 cgagcaaccg caattctgga tcctgcatgg cacacgcagc atcggagaac acacagcgtg
      781 gatggaagcg gcaaccagca ggcaatgcga acggtgccgg gacggtgcct tcgatcgcca
      841 gcaggcgatc atggttctcc tcgatcttcg ggatgctgcc gagcagaccc agcgtgtaag
      901 gatgctgcgg atcgtcgaag atcgccgtcc ctgacgctcg ttcaaccaca cgacccgcat
      961 acatcaccgc cacctcattc gccatttcag cgacgacgcc gagatcgtgc gtgatcagga
     1021 tgatcgccat ccccgtctgc tgctgcagct cacgcagcag atcgagaatc tgcgcttgca
     1081 ccgtcacgtc cagcgccgtg gttggctcat cggcaatcag cagatcgggc gcacaggcaa
     1141 gcgccattgc tatcatcaca cgttgccgca tgccgcccga catctggtgc gggtactcat
     1201 cgaagcggcg ctccggcgcc ggaatccgca cgcgctgcaa cgccgaaatg gcacggctcc
     1261 gcaacgccgc cgacgaggcg ctgcggtcgt gtgcacgcat cgcctctgtg atctgatcgc
     1321 cgacagtgaa taccgggttc aggctggtca tcggctcctg gaagatcatc gcaatgcgat
     1381 tgccgcggat gccacggatc tccgccggcg acagatcgag caggttcttt ccctcgaaca
     1441 ggatctcccc gctggcgatg cgccccggtg aaggaaccag ccgcatgacc gacaacgaaa
     1501 gtaccgactt cccgcagccc gactcgccaa cgatgcccag cgttcggccg cgttcgaccg
     1561 acagatcgac accatctacc gcggcgactt cgccgttgtg ggtgtgaaag accgtacgca
     1621 ggccgttgac agaaagcagc gcgctcattg catgcggaac gtcagaggcg agatctgctc
     1681 cgccgtgcgg tgatcccagt ccagattggg cacgttcccc ttgatgcgtt tctgcgcgtc
     1741 attcagcgcc gcagcagctg caggatagtc cattgtcaat acgcgaccgt cgtccaccac
     1801 cttctggcca tccacgtaaa ccgttcttac agcgcgatca cccgccgcgt aaatcaggct
     1861 gcggattgga tcgtgggacg gccgcatcat cggatgcgtg atgtccacca gcacgatgtc
     1921 tgctcggcag ccgggcgaca gacggccaat gtcgtcgcgc cccaacgcgc gtgcgccgcc
     1981 aatggtcgcg gcttcgaaca gatcggcgga gttcatcgta cgcggattgc ccgcctgagt
     2041 gcgcgccaga tacgacacca gtcgcaactc atccagcata ttgtgcggat acgtgtctgt
     2101 cccgacaccc atgttgacgc ccgcgcgacg gtagcggccg aaatccttca acgtgatccc
     2161 gcgacgggcg aacacagtcg gacaatgcgc cacggtggtg cccgtctcgg ccagccgctt
     2221 cacgtcgctg tcggtgtgcc agcgcgtcga ggggtgatca tccaggaaga tgccgtggcc
     2281 gatgatggag cgctccgaca gcaacccgag atgatcgagc cagccaatag gcgtatagcc
     2341 gtgacgacgc gtgatctcgt ggaactcact gacagattgc gccgcatgga tctgccacga
     2401 cacgccacgc gccttcgcct cggcaaacga ttcacgcagc aatccctcag tgcatgtgtc
     2461 gatctgggcc ggcaccacca tgccgaacaa ccgcccggat ggatgctgct cggcgcgttc
     2521 gatcagcgac agcgcttcgc ccatcgcctt ctcgccggcc gcttcatccc acgcatactc
     2581 caccaggtgc ccgtccttcg tgtaccagcg ccccgagcgg aacatcggcg ccacgcacac
     2641 gcgcattccg gcttccgcca gcagatccaa ccaccccgga tgcgccatcg acaaatcggc
     2701 cagcgtggtc acaccggaca gcaacagctc agacaacgcc acacgcacac aatgcggaat
     2761 cgcctccgca tccgcgcgga agatcggcag atactcgtag agtgaggaat tatagaagcc
     2821 aggactgccg atttcgtcaa tcagcccctt gttcatcggt tcgctggacg ggtgtgagtg
     2881 gatgttgacc aaccccggca tcaccatcat gccggagccg gacactgcct catcggccgg
     2941 cccatcatac ccgcgaccca cgaaccgcag cacgccgcct tcaaaggcaa catccgcatc
     3001 cggcatatac acatgcgact tggtggccgc gtcccaggcg accaccatat cggcattgcg
     3061 gatgacagtg atcaatgaat ttccgccgac cgcttcagaa tttcccgcag cttggccgca
     3121 gaaaaatccg gcgaatggca aaaatccagg atcaccttct cccgtcgtga caccagatcg
     3181 atcgccgccg gcagcttctt caccgcatca gccggcacaa ccaccgcgcc atggtaatcg
     3241 gcatggatca catcatcatg ccccacctgc atgccaaaga tgttcaccgc cttgccgaaa
     3301 tcgaccatgt gcacccaggc gtggctcgga ccaacgcgcc cgccaatcat ctggtagccc
     3361 tccgcccaca tatccatgtc ccggaacgaa ccattcgtca cgcagccgat ggaaccaagc
     3421 gccttgtgga tattggactg cacctcaccc caatacgcgc catagccgat acggccgtcg
     3481 atatcctgga tcaccgcgat ggtcgggaaa tccttcgccg ccacgtactc gtaccagtca
     3541 gcacgatctg tcaccttgcc ccgtggtggc tccgtggagc ggatcatccc gacgcgcgcc
     3601 agcccaacga tcggcttcgc cttcggatac atcgtgacca ttggctccac cgtgtagccg
     3661 aatgcacggc gctccggcga ggtgatctcc agcccattgc agatggttgg cgtgtcccat
     3721 tgggcaagca catcaaggtc ggcctgagtg aatggacgat ccgccatcac ttcctcctgt
     3781 cgttcggttg aggcatggtg acagcatgca gatcatcgcc aagggcggca aggcgctgta
     3841 cggcgccccg ctcggaatcc tcatgctgga ggcgcggttt ccgcgcattc ccggcgatat
     3901 gggcaatgcc gccacatggc catttccggt gctctaccgc gtggtgaagg gcgccacccc
     3961 cgagcgcgtc gtgctgaacg ccgccgccgg cctgctggac gatttcctcg ccgccgcagc
     4021 cgaactggtt gatcagggcg ccgaggcgct caccaccaac tgcggcttcc tctccctgtt
     4081 ccaggccgag ctggcagcgc acgtcaaggt gccggttgcg accagttcgc tgatccaggt
     4141 gccatgggtg caggcaacgc tgccaccggg taagcgtgtg ggaataatta cagtatcggg
     4201 tggatcgctc acgccacggc acctcgccgc ggccggggtc ccgcccgata ccccattcgt
     4261 gggaaccgag gatggccgcg agttcttccg tgtgctggtg ctcggcgaga agcaggacat
     4321 ggacgttgca cttgccgccg ctgacatcct gcaagccgga cgcacgctgc tgtcgcgtca
     4381 tccggatatc ggcgcgatcg tgctggaatg caccaacatg ccgccctacg ccttcgcctt
     4441 gcgcgaaacg ctggggctgc ccgtctacga tatctattca ctgatctcct ggttccacac
     4501 tggcttgcgg ccacgtgaat tcggtccgcc tgcagatcgg gcaacctgcc taccggacgt
     4561 ccctcgccca tagccaacgc gccgcaccgg ctgggtccag ttcgtctcga gccagttcga
     4621 tatgccggcc gaccacattg atccggccga tggtccgcgt gcgatcgagc atgccgcccc
     4681 gcgtcgcgtt caggtgcggg tcactggtga atatgtgctt gatcccttga acgcgcgccg
     4741 catcgatgac cttgttgtcg tatagcccat gcggacagcc cagcgccgag atagtcgcct
     4801 taccaccgga caacacctct acggcctcgc gggcaagccg cagttccgcg gtgacatccg
     4861 gtacacgcgt caacgggaca tggctatagc catgcagccc aatcgcaacg ccatgttcga
     4921 tcagctgttg cagttcgcct ggctccagca tcatccgcgc gtgacagggc gacgctggaa
     4981 tccctgtcag caaccgctcg cgcgccgcct cttccatcac tcccaggcga ctgacgattt
     5041 cgagcccgtt cggttcatca cccagcgccg cgacggcatg gcgtacttca tcccgcgcca
     5101 gccatccatc cagcttgcgc aggcgcgttg cggcaaacac caactcctgc caccagcatt
     5161 ggcccggcga tgcgatggca gccgggactg caaacaccac agctggcagt cccttgcggc
     5221 gcaaagccgg cgctgcatag cgcagattat cagcccaccc atcatcaaac gtgatcagca
     5281 gcgcgcgctg cggcaatggc cgtagtccct cctgcgcgcc gatcacgtca cccaggctga
     5341 caacactgta gtgccggaca acaaagtcga gcagctgttc aaacagcgac aacgacaagg
     5401 tccaggctgg atcggcgctc gcgaaatctg gatcggctcg accaaggacc cgatggagca
     5461 tcaatacggt cagcgtatca cgctgccgga gccgagccag cgacagcgca ccggtatgat
     5521 aggcaagact gcgcagcaaa tggcgtgcac tcattgcgtc agagaaccca ccagttctgg
     5581 cacgggcgcg cctgtagccc tcgcgaggac ggatgtccgg aactccgatg gcgtcatggc
     5641 aacaccgcca agctggtcga tgaagaaacg cagatactcc tcgatcgtct gtagcatccg
     5701 atcgagatcg gcctgcgttc gtacatacgg cgtaaagcca ggcagcagtg ctgaactgtg
     5761 atagttgaaa gtgaacacgc ggtgaccacg acgcaacagc agcttggtca gatgcttcaa
     5821 ctcctggatc gacatgcctt ccggcgtcaa ggtgatccgc tccagcagcc ccagtcgtgc
     5881 gaaaataccg ggcatatgca gcttcgccgc tccgtggctc gacagcgcgg catacaaggt
     5941 cgcgctggag acctgcggct cgctaccgcg cgccaatagc ccggagaagc cggtcgtcaa
     6001 cggaatctcc aagagtttgc gctggcggcc aaaccagtag ggacgggtaa acgcatgccg
     6061 gaagtccggc ccatgccgcg atcgcaaatc gtagcctggc aacacgctga catcgatctg
     6121 ataacccaga tcttccagga tccacgccat ttcatcgcca acaccgtaac ggccagcacg
     6181 ataggatgtc ggcctgatgc cgaagctgct gccgatcaca tcagtcagtt gtcgcagttt
     6241 ttctttttgc agccacgccg gaaggttatg gttgaatgag gtccgcacgc tgagctcttc
     6301 aacgaatggc ggattctccc acggctgcag atgtgcgccg acctcgcagc gccccgattg
     6361 cagcaattca cggatcggct ggtagccttc cggctgcgtc gcgacagcga aatcgacgag
     6421 ataggtcggc cgcactttca agcgatcgaa gatgttctgc gccgatacct gacggctcaa
     6481 attgcgcaca ctgaccagcg aacgcaggaa cggaccatcc caatcaaact ctgcctcggc
     6541 atcgatcagc acgatcagaa ccagaggacc gggtggaata tcggccacgt ccgtcgctga
     6601 tggtcggagc aaaccatcat ccgagagcgt ccggaccctc gggttctcgc tgacctgtgg
     6661 cctgccgccg ggatgtccaa ccagcttctt caggattcgc gccggcggag atgcggccgc
     6721 gagataccaa aggttctctg gtatctgatc gcccaacatc accgcacggc cgatcagcgc
     6781 agcgccacgc aatatcgatc cggtcagata cagatttcga cccaccgatg tgaagcggtc
     6841 gcccaggata ccgcgaattt ctgagtcaga cagacggctt cgctgctcgg ccagatgacg
     6901 ctggatcatc ggcaggacat atgtatccac cttatgcgcg tagaccttgg tcaggctgcc
     6961 ggccgtgtca tgtactgtcg tcaggaattc ctggacgaac tccaccgcgc caatgatcgc
     7021 cagcctgatc cacatgtcct ggtcagccgc gatcgccagc ttggtatcga atggcccaac
     7081 gctgcggagt gccgatagac gcgccacgac gcagggcttg gcaatgaatg tcgctgagag
     7141 aagcgagcgc cagacgtcgg tcggcttgat gccaggcggc agaatgccga attcgcgaaa
     7201 cacattgcct tggccatcaa tgaaacggca gccgcatgtc acaatcacag catccgggct
     7261 attacgcagc accgtgactt gcttggccag tttcgtcggc agccattcgt catccgcgtc
     7321 aagaaacgct acaagttcgc cacgggccgc ggcaatgcct tcattcatgg ccccgctctc
     7381 gccacgattc tgcggcaagc ggaacagacg tatgcgctca tcgcgatagg cagcgacaat
     7441 ctccgaagta gtgtcttggc tgccatcgtc caccacgatg atctcgtaat cgctgaaagt
     7501 ctgggccaac accgaattga tggctcgtgc gatcgtcgcc gccgcgttgt aggccgggat
     7561 cagcacagtg accaatggag cggtcatatt cacctactgc gcgtcaatgt tctgtcgtcg
     7621 cctcgcgatc acccgcctgc gccatgctcg caggcgccac tccatggaaa gccatgaacg
     7681 cgcggccatc gagcgcatgg taccggcata ccagttcccg cggccgtaac gcgtcgttcc
     7741 agtagcaaat tcacgcttgt atggtgcatc accaacgccg cgatcgatga gcttgatccc
     7801 gtcctggggc gcacggcgga tcgtctgcca caacaaaagc cgccccggcg agatgttccg
     7861 cacctccgga tcatacacag gaaaccaata attgagaaca tcctgacact gcggccccag
     7921 atgctgtgca agcacccgac tgttcgcttc cagcctggtg aggaccagcc ggcaatccag
     7981 ttcggctgtc tcgttcatcg cgctgatcac gcgcatgctg tcgccagcgc tgaatggatc
     8041 cgccacctcg gtccgcttat attgtgcgcg cttctgcgcg atgatctcgg caatcagcgc
     8101 gcccggcacc ggatcgaacc tgaccacctc caagcggcca agatcccgct cggcgcggcg
     8161 cagccgccgc tccgtatccc gcacgaagtc acggctgcgc tcgccgactg cagcaaaata
     8221 gctgtcgcta tccgccccca catcggtgac atagccggta tcacaccacg ctgcatccag
     8281 gccgaactgg ccctgatctt caacaagatg cgtcacgaac atagaggcca ggcctgacac
     8341 acgcatcagt gcgccagcat ccgctgtgaa tccaacccgt gcgatcaggc ccgccgcatc
     8401 cgatatgcct ccacctatac gctccgcgag ccgcaggcgc tgatgccagg cggtcgcgaa
     8461 ctggaacggc agaaacgctt tgatcccatc cccgtcgtaa accaccgaca cataggcacg
     8521 gtccgtcgcc cgttcgcagg ccaaggcaaa ggctggtgaa aaaaatgcac gctgcagcga
     8581 acggcgcgcc ttcatcatct cctgccacag cgaaatctcg ctcgccccca gagccgacgg
     8641 tcgcatcacg tccgcccgga tcgagctgct atccatcgac aggcattcgc actcgggcga
     8701 ggcaggccaa tggcagctac gcgttcttct tcacgccata gaactgcggc cacccatcgc
     8761 gcacatccgt cagataattg tggaaggccg tgggctgcac atacatgcca agcggtgcat
     8821 agggcgggct ctgccagaac tccgcttgca tctcgtcgca gatcttcttc tgagccgcca
     8881 catccggcgc gttgaaccac gcagtccgca gctcctccat cttcggatcg gtgggccagc
     8941 cgaaccagct cccattggtg ccgctcgact ggatcgggat cgccgccgcc ggcgtgatgt
     9001 tcccgaagcc gcccagatac gtatagaaaa tgttccagcc gcccttgtcg atcggctcct
     9061 tgctggcgcg gcgctgcacc accgtgcccc attccagcga ctggaaatcg gtgttgaagc
     9121 cgatcttctt cagcgtatcg gacgccacct gagcctcggc ccagatggta gggatcgtgg
     9181 acgccgcaag gatcacgatc ttctcgccct tgtagcccgc cgcgaccagg tccttcttca
     9241 gcttgtcgat attcttgggc ccgcgcgtga tctccacgcc aagcttgctc gccatcggcg
     9301 tgcccggcac gaagatgccg acgtcggtcg tgatcaggct cggatcggca ccggcgaccg
     9361 cttccatgat ctccttctgg tccatggcat ccaccacaac ccgacggatc gccggattgt
     9421 cgaatggcgg gaacaggtgg ttgaagcgca ggcagccaat ctccccggtc cggtccttca
     9481 ccgcgagcgt caggtccttg tcacgccgaa gctgcggcac caggtcgatg gttgggttct
     9541 cccaccagtc gatttccttc tgggccagcg ccgctgccgc cgtcgccggg tccggaatga
     9601 aattccacac cacgcgatcg atgtagacga tcttcggacc tgcattgaag ctcggcacgc
     9661 cgtccttgcg ggggacatac ttgtcgttct tcacataaac cacccgctgc cccggcacgc
     9721 gctccgaggc gacgaacttg aacgggccgc tgccaacggc ctccgccacc tgctgcatcg
     9781 catccgtctt cgccagacgc tccggcatga tcgcgcaata cacctgagca agcgcgttcg
     9841 gcagcagcgc gaaaggctgc ttcagcttga agcggatcac cttgtcagtg ggggccgaga
     9901 tctcatcggt gcgtgccatc aaagccccgc cgaacggatc acgcttggcg aaccgctgga
     9961 tcgtcgcgac gcaatcgcgc gccagtactg gcgtgccatc atggaatccc aggccatcac
    10021 gcagggtcag gtcccactgc ttgccatcgt tgctgatgac atggccctca accatctgcg
    10081 gctgcacggt gaagccagcg tcgtagccgt acagcgtgtc gaatacggca agcgagaaat
    10141 tgcgggtgat gtccgccgtg gtccatacgg gatcgaggga agccaggtca gcgtgcggcg
    10201 tgaagttgat ggtcttggcg ccctcggcat gcaccacacg cggtgcggcc agcgccgcgg
    10261 cgccggcaac tgcggatttc aggacatcac gacgtttcat ctattggccc ccttggatcg
    10321 gctgatcttc taaggcagcg cgcggccgtc agcaattatt ctcggcgtgc agggcacgtg
    10381 caacgacagg cgccgccgcg gccgacatcc gcttgccgtc atggcctacg tccggcacgt
    10441 tgatcacggt ccaggcacag cgcgtgccaa gcgcctcagc cgcggcgtga ccggcgcgga
    10501 catagttgtg cgcacgttcg tgccgggttg cgccctggtg catcgagcgc ggccctttcg
    10561 gaaaataccg gcccgtcgtc ttgatgtccc cagtgccggt catcacggtg atcgggaatt
    10621 ccaacaggcg gcccaatgcc ggcggatcga gctctgtctc gccaagcccg aacggccagg
    10681 cgacatccag gtcaggcatg gcataggtgc cggcattggc actcaccgcg acagcgacat
    10741 gatcccgata gccgaacgac agcatgcgat gcacatactg gccgccggcg gaatggccga
    10801 acagcccata acgctgctgg ctcgtcaccc cctgcgcccg cagcgccgcg aacaggcgtt
    10861 cgtcgactcc ataggtccac tgctcagacg gattgagcgt gccgtcctcg tcatgacgat
    10921 tgccgaagtg ataccagaga tagtcgggga aaaacgcttc cgagaactcg atagcgatga
    10981 caaagacctg gatctcatcg accagcctca gccagtaatc gcgatagtcc ctgccgttgc
    11041 ggccaacgcc atgatgtacg aacagtaccg gcatctcacg cgaccagtcg cgcggtcgcg
    11101 ccgcatgcag cgtcaatgga cgatcaggaa acgccggatc ctgataccgg atcgaatggc
    11161 cgccaggccc ggcaacgagg gctgagagat cagtcatggc cgcaaatcct gcaacgcccc
    11221 tggtcaactg gccagtatca atttcgccac ctgatctgac atacattcca gcgaacgcag
    11281 ggaggggcac actatgcaat tggcaatcat cggtgccggc aatgtcggca gcgcgctggc
    11341 cgctggctgg agcgccgcgg gtcatcgcat cactttcggc gtgcgcgagc cgcaagccgc
    11401 accgtccggc ggagcgacag gcacaaccta tgcaagcgta gcggcggccg cgaagagtgc
    11461 cgaggtcctc attttgtccg ttccctggca ggccgtgccg gaggcacttg catcagcagg
    11521 cgatttgacc ggcaagatcc tgatcgactg caccaatcct ctgcagatgg gaccggacgg
    11581 gctcgaactg gctatcggcc aaacgagttc aggagccgaa cgtgtggcag aactcgcccc
    11641 cggagcctcg gtgttcaaga cgctcaacca gaccggcttc gccaacatgg agcacgctca
    11701 cgaattcgcc ctgcctgctg tcatgtacgt cgcgggcgac gacgaaatgc gaaagcctct
    11761 ggtaatgtca ctggttgctg aacttggctt ccaggcggtc gacgccggtc ggctgcgcat
    11821 cgcccgcctt ctggagccga tggcgatgct gtggatccac atggcgctca atcgcggcga
    11881 gcctctgaca actgcattcg cgctgtcccg acggccgtag ctacagatat accccgcgct
    11941 cgaggatctc cagcgtatat tcgcgatagc tgattcccag cgcctcggcc cgcgtcaggc
    12001 gccgcatcgc gatctcgcgg cctggcgtct tccacgcacg cttcaccgcg cgcttccagc
    12061 accagtgccg ccacgactcg ttcggatcga tgccgggatc gagcggggga ccgccgttgt
    12121 cgccgatccc ccggaccggt tcagtaagcc cgtgcaacgg cgcgcttcgc catcacaccc
    12181 accaggtgcg cgcgatactc ggcgctggcg tggatgtcgc tgttcagtcc atccgccggc
    12241 gtggtgatcc cggcgatcgc atcaggcgag aaattcgccg tcagtgcctg ctccatcgcg
    12301 gtatggcgga acacgccgct ctgcccggcg ccggtcaccg ccacgcgcac gccgccagcg
    12361 gtcttcgcca cgtacacacc gacgatcgca tagcgcgacg ccggattgcg gaacttctca
    12421 taggccccct tctcgggcac cggaaagtcc accgccgtga tcagttcgcc ctgctccagc
    12481 gccgtggtga acatgccctg gaagaagtca tccgccttga tcgagcgctt gttggttttg
    12541 atcgtcgccc ccagcgccaa cgcggccgcc ggataatccg cggcaggatc attgttcgcg
    12601 atcgagccgc cgatcgtgcc acggtgccgc accgcctcgt cgccgatatg cgaggcaagc
    12661 tccgccagcg acgggatctt gcccttcagc ccggcagcct gcgccaccgc gccatgcgtc
    12721 gtcataccct ggatcgtcac cgtgttgccc gacacgctca cgccaaccag gccgagtttc
    12781 gccaggtcca ccagcaggct cggcttgttc aatcgttgct tcagcaccgg gatcagcgtc
    12841 atgccccccg ccagcgcctt cgcatcgcca tcctcaacaa gcgccttcac cgcatcggcg
    12901 aggctcgatg gcttctggta tgtgaaatca tacatcgtgc tgttcctcgt tccgattact
    12961 tcgcagccat gagcgaccag agtttctgcg gcgtgaccgg catgttgatg tgccgcacgt
    13021 tgtagtccct cattgcatct acaatcgcat tgacgatcgc aggcggcgag ccgatcgcgc
    13081 cgacctcgcc gcagcccttc gaacccagcg gattgtgcgt gcagagcgtg atctcggtac
    13141 ccaccgtgat attcggcagg tcatccgccc gcggcatcgt gtagtccatc atcgagccgg
    13201 acagcagttg cccggcaccg tcatacaccg cgttctccag cagcgcctgg cctatgccct
    13261 gcgccacgcc gccctgcacc tggccctcga cgatcatcgg gttgatcacg cgcccgacat
    13321 cgtcgacggc cacgaagttc accacctcga cggtgccggt ctccttgtcc acttccactt
    13381 ccgcgacgtg gcacccgcca gggaaggtga agttcttcgg atcatagaac gccgtctcgt
    13441 ccaggcccgg ttccagctcc tcgatcggat agttgtgcgg cacatatgcg gccagcgaga
    13501 tctcagtcag cgtcttttcc ttgtcggtgc cggcaacgct gaacttccca tccttgaact
    13561 cgatgtcctc caccgaggct tccatcagat gggcggcgat cttcttgcct ttggcgacga
    13621 tcttgtccat cgccttcacc atggcggaac caccaaccgc gaggctgcgg ctgccgtagg
    13681 tgcccatgcc gaacgggatc ttgcccgtgt caccatgcac cacctccacc tggtcgagcg
    13741 gcacgccgag ctggtccacc acaagctgcg ccatcgtcgt ctcgtggccc tg
//
LOCUS       DATGEM010000286         7314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_852895, whole genome shotgun sequence.
ACCESSION   DATGEM010000286 DATGEM010000000
VERSION     DATGEM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852895"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VKQ27_09045"
     CDS             complement(<1..466)
                     /locus_tag="VKQ27_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HLY89118.1"
                     /translation="MDSSSMGRRSERVAVNSGKFLELEGLRGLAAAMVVAWHFVWAFA
                     PSELGSVAGSPEQGLVGSPIAAGIDGPAAVVLFFVLSGFVIPLRFFRSGRARTVVHAA
                     TKRWPRLAGLSVLAVLFSYGLFRFGLFHYREAAALTGSDWLGAYGGGDPGGTL"
     gene            complement(813..1193)
                     /locus_tag="VKQ27_09050"
     CDS             complement(813..1193)
                     /locus_tag="VKQ27_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HLY89119.1"
                     /translation="MPAEPLGILLLSGTHDRAHYAFVLASGAAAIGRQVVLFATNAGC
                     HAVLTDWSGLGDAARDERAREAGVAGLAELRDAARELGVRMIACEAGLRVENIEPARL
                     MPGVEVAGVATFLSSVGAGQIIAV"
     gene            complement(1183..1998)
                     /gene="moeB"
                     /locus_tag="VKQ27_09055"
     CDS             complement(1183..1998)
                     /gene="moeB"
                     /locus_tag="VKQ27_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLY89120.1"
                     /translation="MDLDFSEDEIRRYSRHILLQDVGGIGQARLKAARVLVIGAGGLG
                     SPLALYLAAAGVGTIGIVDHDRLELSNLQRQIAHTTDRIGQPKALSAAQTAHAINPEV
                     RVEPHDVRLDADNAAELIGRYDIVCDGTDNFVTRFLVADACVLARRTLISAAVLRFEG
                     QLSVFKPHVEGPCYRCLYPEAPPEGAVPTCSEAGVLGAVTGVMGTLQATETLKEILGI
                     GETLAGKLLIWDALATRFRTVRLRPDPTCALCSPAATIKDLSEHRHAVVACAG"
     gene            2085..2825
                     /gene="queC"
                     /locus_tag="VKQ27_09060"
     CDS             2085..2825
                     /gene="queC"
                     /locus_tag="VKQ27_09060"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HLY89121.1"
                     /translation="MSPDHALVLFSGGQDSTTCLAWALARYPHVETVGFAYGQRHAVE
                     LDCRAPIRSAIGAMWPGRLGPDYTVDLAATIGGLGATALTSETTIAMTEEGLPNTFVP
                     GRNLLFLTAAAALAYRRGLRRIVGGMCETDYSGYPDCRDDTIKAMQLALNLGMQRRFV
                     LETPLMWRDKAATWELAQSLGGDALVELIRHETHSCYLGDRSRLHAWGYGCGTCPACE
                     LRANGWQRWRASAASASINVTSFSGDSA"
     gene            2822..3190
                     /locus_tag="VKQ27_09065"
     CDS             2822..3190
                     /locus_tag="VKQ27_09065"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HLY89122.1"
                     /translation="MKFVLPLAVLAVALAAPAAQAQRGISIQARTAGELAELCAANPK
                     DAQGDAKINFCQGYAQGALSTELRHTEGKKLFCFPSPAPTRSATMAQFVDWVRALPDH
                     KSLPAVDGFFRFLGERFPCK"
     gene            complement(3200..3355)
                     /locus_tag="VKQ27_09070"
     CDS             complement(3200..3355)
                     /locus_tag="VKQ27_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89123.1"
                     /translation="MTEDLHQTIEELRAAVQGRDDLIAIAAHALRDPMTPIASRVDLL
                     LAAVRTR"
     gene            3583..4005
                     /locus_tag="VKQ27_09075"
     CDS             3583..4005
                     /locus_tag="VKQ27_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLY89124.1"
                     /translation="MALFPDPFGTLLGLQNTLDAFRRSDWLQSGPSAGGSYPPLNVFR
                     KGDDFTLVAELPGISSADLDIQVKGRTVRLSGSKPVKYPEKASVHRRERLQGRFDRSI
                     TLPVEVDQDRAAAEYRDGILAITLPRAERDKPRSIRIG"
     gene            4017..4415
                     /locus_tag="VKQ27_09080"
     CDS             4017..4415
                     /locus_tag="VKQ27_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLY89125.1"
                     /translation="MSGSQELAVREKQELARQEEQTVPGRYYVPAADIFETDDALTLL
                     LEMPGVSKDNVDVQIENDVLRVEGKIDYATYKDIEPVYTEYNIGHYARAFTLSNKIDR
                     DAISARVEDGVLTLTLPKAKEAMPRRITIS"
     gene            4491..4865
                     /locus_tag="VKQ27_09085"
     CDS             4491..4865
                     /locus_tag="VKQ27_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HLY89126.1"
                     /translation="MRQDRVTHHRAVDIAVLVCVVGLAVATWAAISDTGRTSTWRPCF
                     TISATIFLALAAATPHATWAAAMRLAMSGWLMMAPWLLAFADLPLARWSHVIAGSLIA
                     ALSAPRLLRSPALLTNQDAGCT"
     gene            4957..5124
                     /locus_tag="VKQ27_09090"
     CDS             4957..5124
                     /locus_tag="VKQ27_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89127.1"
                     /translation="MQIDTATPTDHLSNQAIIERCSETRQTDENADPPWPLLSRPMQE
                     PIVWPRVFPGL"
     gene            complement(5200..6051)
                     /locus_tag="VKQ27_09095"
     CDS             complement(5200..6051)
                     /locus_tag="VKQ27_09095"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HLY89128.1"
                     /translation="MTTGKIAFQGVLGAYSDLACRVAYPDRETLPCESFETAMDAVRE
                     GRADLAMLPCENSLAGRVPDIHHLLPESGLFVIGEHFQRVEHCLLAVKGASIEGLKRA
                     HSHTVALGQVRHSLRRLGLTPVVQADTAGAAELVAGWGRREEAAVASSLAAEIYGLKI
                     LAANIEDAAHNTTRFYVMARQPKTPSVSEPNLMTTFVFRVRNVPAALYKALGGFATNG
                     VNMTKLESYMLEGHFVATQFLCDADGHPEQPGLRRALEELGFFSSEVRVLGVYPAAPF
                     RLAQGAG"
     gene            complement(6048..6794)
                     /locus_tag="VKQ27_09100"
     CDS             complement(6048..6794)
                     /locus_tag="VKQ27_09100"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HLY89129.1"
                     /translation="MNPVIVIPSRLASTRLPGKPLADIHGKPMIVHVMERAQEATIGP
                     VAVACADPEIAEAVRAAGGRAVMTNPDLATGSDRVYAALAEIDPDGRHDVVVNVQGDF
                     PTVTAAQLRAVVLPLVDQEVDIGTLVTPIASDEEAHTASFVKAACAFDEGQLVAPALY
                     FSREAIPWGEGPRWHHIGIYAYRRQALARFVLLPSAPLERRESLEQLRALEAGMRIAC
                     ARVDYGPFGVDTPADLERARRVLDPKRKAG"
     gene            6937..>7314
                     /locus_tag="VKQ27_09105"
     CDS             6937..>7314
                     /locus_tag="VKQ27_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLY89130.1"
                     /translation="MDTMEVNKVIAAVLVGGILFFLTGLLGDHLVHQTELEKTVLAIA
                     AAPEPSGGPAAPTGPGDITPLLASADVKVGEQFTHRVCIACHTFDKGGKNGVGPNLYG
                     VVERPRDSEPGFSYSPAMEKFKGK"
BASE COUNT         1337 a         2644 c         2165 g         1168 t
ORIGIN      
        1 acagcgtgcc gccggggtcg cccccaccat acgcgccaag ccagtccgac cctgtcagag
       61 cggcagcctc ccgatagtga aacaggccga aacggaacag cccgtaggag aacagcacag
      121 caaggaccga cagaccggcg agcctcggcc agcgcttggt agccgcatgc acgacggtcc
      181 gggctcggcc ggaacggaag aaccgaaggg gaatgacgaa cccggagagc acaaagaaca
      241 atacgaccgc cgccgggccg tctatgcctg cggcgattgg gctcccgacc agcccctgct
      301 caggcgaacc cgccacgctg ccaagctccg atggcgcgaa tgcccatacg aaatgccatg
      361 caaccaccat cgcggctgcc agcccacgca gcccttcaag ctccaggaac ttgcccgaat
      421 tcacagcaac gcgttcactg cgacgcccca tactggaaga gtccattgcg ctcatcacag
      481 gaggcaggtc ctacgcggcg attttaacta ttcgccaggt cactgtcggc cgccgcgttc
      541 gcgacatcct aaagactatc gtaggtaaat atcggcgctc tgccgcactg cgccgttgtg
      601 ggttcggaac agtcaggaag agcagtcgca tcgcgctaaa acgcacgggc gccggcccgg
      661 cagcaatcgc ggcacgttca actccgtttg agcgcccgtg cttgtcagac ctcggcccga
      721 tttggccgaa cacgaaagcc ggcgtctcgc acgtctcctg cccgaatgcg caatcggcgc
      781 ttcagcaaag gcgatgaaac actcaccgcc tctcagaccg cgatgatctg ccctgcgcct
      841 accgatgaga ggaacgttgc gacaccggca acctcgacgc ccggcataag ccgcgctggt
      901 tcgatgtttt cgacccgcag gccagcctcg caggcgatca tgcgcacgcc gagctcgcgc
      961 gccgcgtcac gcagctctgc cagacccgcg acacctgcct cacgggcgcg ttcatcgcgc
     1021 gcggcgtcgc ccaggccgga ccaatcggtc agcaccgcat gacacccggc attggtcgcg
     1081 aacaacacca cctggcgccc gatggccgcg gcgcccgagg ccagcacaaa ggcgtagtga
     1141 gcccgatcgt gcgtgccgga cagcaacagg atgccgagcg gttcagccgg cacaggcaac
     1201 gacagcgtgg cggtgctcag acaggtcctt gatcgtcgcg gcggggctgc agagcgcgca
     1261 ggtcgggtcc ggcctcagcc ggacagtgcg gaagcgggtc gccagcgcat cccagatcag
     1321 cagcttgcct gccagcgttt cgccgatacc aaggatctcc ttcaaagtct cggtcgcctg
     1381 taaagtgccc atcacgccgg tgacggcgcc gagcacgccc gcctcggagc acgtcggcac
     1441 cgcgccttcc ggcggcgcct caggatacag gcagcgataa cacggtccct cgacatgcgg
     1501 cttgaacacc gaaagctgcc cctcgaagcg cagcaccgcc gccgatatca aggtgcgacg
     1561 cgccagcacg cacgcatcgg ccaccaggaa ccgcgtgacg aagttgtccg tgccgtcgca
     1621 cacgatgtcg tagcgtccaa tcagctcggc cgcattatcc gcatccagcc gcacgtcgtg
     1681 cggctcgacg cgcacctcag ggttgatcgc gtgagcggtc tgggcagccg acagcgcctt
     1741 gggctggccg atccggtccg tggtgtgcgc gatctggcgc tgcaggttcg acagctccag
     1801 ccggtcgtga tcgacaatcc cgatggtgcc gacaccggct gccgcgagat acagcgcgag
     1861 cggtgaaccc agcccccccg cgcctatcac cagtacgcga gccgccttga gcctggcctg
     1921 gccaatgccg ccgacgtcct ggagcaggat gtggcgcgaa taccgacgga tttcgtcttc
     1981 ggagaaatcg aggtccatgg gcagaaggat agtgcgcgcc ccgtgtcatt gcgaggagca
     2041 gcgcgacgaa gcaatctccg gcaccttgtt catcgcagtt gcacatgtcg cctgaccacg
     2101 ctctggtgct gttctccggc ggccaggaca gcaccacctg tctggcctgg gcactcgccc
     2161 gctatccgca tgtcgagacc gtcggcttcg cctacggcca gcgtcacgct gtcgaactcg
     2221 actgccgcgc cccgatccgc tccgccatcg gcgccatgtg gccgggccgc ctcggccccg
     2281 actacaccgt cgatctcgcg gcgacgatcg gcggcctggg cgccaccgcg ctgaccagtg
     2341 aaacgacaat cgcgatgacc gaggaaggcc tgcccaacac cttcgtcccc ggccgcaacc
     2401 tgctgttcct cactgccgcc gcggcgctgg cgtaccgccg tggcctgcgc cgcatcgtcg
     2461 gcggcatgtg cgagaccgac tactccggct accccgactg ccgcgacgac accatcaagg
     2521 cgatgcagct tgcgctgaac ctcggcatgc aacgtcgctt cgtgctcgag acaccgctga
     2581 tgtggcgcga caaggccgcc acctgggaac tggcccagtc gctcggcggc gatgcactgg
     2641 tcgagctgat ccggcacgaa acccacagct gctacctcgg cgaccggtcg cgcctccacg
     2701 catggggcta tggctgtggc acctgcccgg cgtgcgagct gcgcgccaat ggctggcagc
     2761 gctggcgcgc gagcgcagcc tcggcttcaa tcaacgtcac atccttttca ggagactccg
     2821 catgaagttc gtcctcccgc tcgctgtcct ggctgtggcc ctcgccgcgc cggcagccca
     2881 ggcccagcgc ggcatcagca ttcaggcgcg caccgccggc gaactcgcag agctatgcgc
     2941 cgccaatccc aaggatgccc agggcgacgc caagatcaat ttctgccagg gctatgccca
     3001 gggcgccctg tccacggagc tgcgccacac cgagggcaag aagctgttct gcttcccgag
     3061 cccggcaccg acccggtcag caaccatggc gcagttcgtc gattgggtgc gtgcccttcc
     3121 cgaccacaag tcgctgccag cggttgacgg gttcttccgc ttcctgggcg aacgcttccc
     3181 ctgcaagtag cttggcgagt caccgcgtcc gcacagcggc gagcagcagg tccacccggc
     3241 tcgctatcgg cgtcatcggg tcgcgcagcg catgcgcggc tatcgcgatg aggtcatcgc
     3301 gcccctgcac cgccgcccgc agttcctcaa tagtctgatg caaatcctcg gtcacgttcc
     3361 gccggcgatt ctgcaggtct gctagttggg cagagccggc ggattcacga agtaatccct
     3421 ggttcatccc cgcttgacgc cagcatcgca atgcgtggcc tcaacgaatc cccttgacgc
     3481 gccttctccg aaaactacct cactggcact cgaacgcagg gagtgctaac gccgcggtcg
     3541 caccctccct tggttcgaag caacagatag ggagaggtca caatggcatt gtttcctgac
     3601 ccattcggta cactcctggg tcttcagaac accctcgatg cattccgccg cagcgactgg
     3661 ctgcaatcag gcccaagcgc cggcggcagc tacccacccc tgaacgtctt ccgcaaaggt
     3721 gacgacttca ccctcgtcgc cgaattgccg ggcatcagca gcgcggacct ggacatccag
     3781 gtgaagggcc gcacagtccg tctgtccggc tcgaaaccgg tgaagtatcc agagaaggcc
     3841 agcgtccatc gccgcgaacg cctgcagggt cgcttcgatc gttccatcac cttgccggtg
     3901 gaagtcgacc aggatcgcgc cgccgccgaa tatcgcgacg gaatcctcgc gatcacgctg
     3961 ccgcgcgcgg aacgcgacaa accccgttcg atccggattg gctgaaggag gcgacgatgt
     4021 caggttctca ggagctggct gtccgtgaaa agcaggaact ggcgcggcag gaagagcaga
     4081 cggtccccgg gcgctactac gtcccggccg ctgacatttt cgagacggac gacgcgctga
     4141 cacttctgtt ggagatgccc ggcgtcagca aggacaatgt tgacgtccag atcgaaaacg
     4201 acgtgctgcg tgtcgaaggc aagatcgact acgcgaccta caaggacatc gagcctgtct
     4261 atacagaata caatatcggc cactacgcgc gtgcattcac cctgtcgaac aagatcgacc
     4321 gggatgcgat cagcgcccgg gtggaggacg gcgtgctgac cttgacgctg ccaaaggcca
     4381 aggaggccat gccccgccgg atcaccataa gctgacgaac cgccggatcc cgccacggcg
     4441 gcggggtccg gccttcaacc tgacggtgca agcaaaggag gatcggaacc gtgaggcagg
     4501 atcgtgtgac gcaccatcgt gccgtcgaca ttgccgtgct ggtgtgcgtg gtggggctcg
     4561 ccgtcgcaac ctgggccgcg atatctgata ccggcaggac ttccacctgg cggccctgtt
     4621 tcaccatatc ggcaaccatt ttcctggcgc ttgccgctgc gacgccgcac gcgacgtggg
     4681 ccgcggctat gcggctcgcg atgagcggct ggctcatgat ggcgccctgg ctgctggcgt
     4741 ttgccgacct gccgctcgcc cggtggtctc atgtcattgc cggttcactg atcgccgcat
     4801 tgtcagcgcc acgcttgctg cgcagtccgg cattgctgac aaaccaggat gcaggctgca
     4861 cttgaagtgc cgcaccggaa aactatctgg ccggtgccgg gaggccttcg gggctcggca
     4921 ctgaacagtc catgttgctc tcaggaggat gtggtcatgc agatcgatac cgcgacacct
     4981 accgatcacc tgtccaacca ggccatcatc gagcgctgca gcgaaacccg ccagaccgac
     5041 gagaatgcag acccgccgtg gccattattg tcacggccaa tgcaagagcc gatcgtctgg
     5101 ccgcgggtct tccccggtct gtagcaaagg cacccggatg cacgcgggga ctgcggcctc
     5161 tgtccgcgtc catccgcgtg cagctgcggt tccagttccc tagcccgccc cttgcgccag
     5221 tctgaacggc gccgccggat aaacgcccag cacgcgcacc tccgaggaga agaaccccag
     5281 ctcttccaga gcccgccgca gccccggctg ctccggatgc ccatccgcgt cgcacaggaa
     5341 ctgcgtcgcg acgaagtgcc cttcgagcat gtagctctcc agcttcgtca tgttcacgcc
     5401 gttggtcgcg aacccgccca gcgccttgta cagcgccgcc ggcacgttcc tcacccggaa
     5461 cacgaacgtc gtcatcaggt tgggctcgct caccgatggc gtcttcggct gccgcgccat
     5521 cacatagaac cgcgtcgtgt tgtgcgctgc atcctcgatg ttcgccgcca ggatcttcag
     5581 cccatagatc tctgccgcca gcgacgacgc gaccgccgcc tcctcccgcc gcccccaccc
     5641 ggcgaccagc tcagccgccc ccgcggtatc cgcctgcacc accggcgtca gccccagccg
     5701 gcgcaggctg tgccgcacct gccccagcgc cacggtatgc gaatgcgccc gcttcagccc
     5761 ctcgatcgag gcgcccttca ccgccagcag acagtgctcc acccgctgga agtgttcgcc
     5821 gatcacgaac agccccgact caggcagcag gtggtggatg tccggcaccc gccccgccag
     5881 gctgttctcg cacggcagca tcgccaggtc ggcgcgcccc tcgcgcaccg catccatcgc
     5941 cgtctcgaac gactcgcacg gcaacgtctc cctgtccgga taggccaccc tgcatgcgag
     6001 gtccgagtac gcccccagca ccccctgaaa cgctattttt cccgtcgtca tcccgccttc
     6061 cgcttcggat ccagtactcg cctggcgcgc tccaggtccg ccggcgtatc gaccccgaac
     6121 ggtccgtaat caacccgcgc gcaggcaatc cgcatccccg cttccagagc acgcaactgc
     6181 tccaggctct cccgccgctc cagaggagca gacggcagca gcacgaagcg cgccagcgcc
     6241 tgccgccggt aagcataaat gccgatatgg tgccagcgcg gcccctcgcc ccatgggatc
     6301 gcctcccgcg agaaatacag ggcaggcgcc accagctgcc cctcatcgaa cgcacatgcc
     6361 gccttcacga acgacgccgt atgcgcctcc tcatcactgg cgatcggcgt taccagcgtc
     6421 ccgatgtcca cctcctgatc gaccagaggc agcaccaccg cccgaagctg cgccgccgtc
     6481 accgtcggga aatccccctg cacgttgacc acgacatcat gccgcccgtc ggggtcgatc
     6541 tctgccagtg ccgcgtaaac acgatcggat cccgtcgcca ggtccgggtt cgtcatcacc
     6601 gcccggccgc cagccgcgcg caccgcctcg gcgatttccg gatccgcaca cgccaccgcc
     6661 accggaccaa tcgtcgcctc ctgcgcgcgc tccatcacgt gcacgatcat cggcttgcca
     6721 tggatgtccg ccagcggctt gccaggcagc cgcgtcgagg ccaggcggga aggaatgaca
     6781 atcaccggat tcacaaaagc accacgccca cggtgggttg atgggtaagg accgtttccc
     6841 tatggtgcgc gcaccttatg gaggcgaacg gccgcgtgca acggctggcc ggtgaacctc
     6901 catgcacgca tgtttggact ggggaagtca cgccgcatgg atacgatgga ggtcaataag
     6961 gtcattgccg ccgttctggt gggtggcatc ctcttcttcc tcaccggcct tctcggcgat
     7021 catctcgtcc accagaccga actggaaaaa accgttctgg ccatcgccgc cgcccccgag
     7081 ccgtccggcg gcccagccgc gccaaccgga cccggcgata tcaccccgct gctcgcctcc
     7141 gccgacgtca aggtcggtga gcagttcacc caccgcgtct gcatcgcctg tcacaccttc
     7201 gacaagggcg gcaagaacgg cgtcggcccg aacctgtatg gcgtggtcga gcgcccgcgc
     7261 gactccgagc ccggcttcag ctattcgccg gcgatggaga aattcaaggg caag
//
LOCUS       DATGEM010000287        29979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_339871, whole genome shotgun sequence.
ACCESSION   DATGEM010000287 DATGEM010000000
VERSION     DATGEM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 29979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29979
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339871"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VKQ27_09110"
     CDS             complement(<1..603)
                     /locus_tag="VKQ27_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY89131.1"
                     /translation="MAALAPGVMSADEAALWSALRIDRDDAARQHLFERHAGFARSIA
                     RRQFNGRSQGDLEFQDVHQWACTGLLEAIDRFDPTRGVAFRTYAAHRISGSISNGIST
                     MSEVREQQSARARVRRDRLRSLAPGDVVGLSDADALQSLAAIAMGLAVGFMLEGTRLY
                     VEDEEETIVSRDQANGYDSAAWYELTERLLVEIESLPSRER"
     gene            complement(612..1367)
                     /gene="fliP"
                     /locus_tag="VKQ27_09115"
     CDS             complement(612..1367)
                     /gene="fliP"
                     /locus_tag="VKQ27_09115"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HLY89132.1"
                     /translation="MTALLLVGWLTPCQTIAAAPARAAESSMLGTPTQNSALTRVEPQ
                     TAQTVRIALILSLMAILPALVICTTSFTRIIVVLSMIRHGLGMPETPPNQVLVSLAIF
                     LTMFSMAPTFEALNDHALQPFLAGRMTVDQAAQAGGAPLREFMLRQVHDQDIKAVYDI
                     ARAPLPARAADVGFMQLVPAFMLNELRVAFRIGFVILLPFLLIDIVVSSILLALGMLM
                     VPPSTLSLPIKVLMFVLIDGWSLILQGVLGSFH"
     gene            complement(1406..1777)
                     /locus_tag="VKQ27_09120"
     CDS             complement(1406..1777)
                     /locus_tag="VKQ27_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89133.1"
                     /translation="MRAPVRYARWFGALAVLSASGPAFAQLANPQDDGVSFGRVVGAL
                     LLCLGLAVAGALAIRARLGGGLALTLPGKSPRRLRLHESLRVSPQVQLAIVACDGREM
                     LISTSASGAQLITHLPPEIRQ"
     gene            complement(1774..2076)
                     /locus_tag="VKQ27_09125"
     CDS             complement(1774..2076)
                     /locus_tag="VKQ27_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HLY89134.1"
                     /translation="MTDKNNPLPATADASAAKPIVAIDQELFRDVRVDIEARLGRAEL
                     SVDALMALKSGSVVTLSTGLADHVDLYVNDACVARGEIVAVGDKFGVRIVEVAAKQ"
     gene            complement(2094..2825)
                     /locus_tag="VKQ27_09130"
     CDS             complement(2094..2825)
                     /locus_tag="VKQ27_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HLY89135.1"
                     /translation="MSSAARAWLPDGAFERSAARRVLSPVLEAWSNDWFVDAMISLAD
                     VREADRATGLHGLVVDRPGQPTTISLTARGKRLLFETACSVDLAEIELSDTDRRLVDI
                     LVEEIASDLLARLDAMLEVPRVHDTPSHSLVLTLKGRDLLTINVSRSLLASWLKSGMP
                     QPRGREGTLTKRSRAIGETTLSLDAVVGSASIALADLTHLAIGDILILDQALDAGVAM
                     TLSSSGRPIVHGQLGREDDKPTLQF"
     gene            complement(2825..4018)
                     /locus_tag="VKQ27_09135"
     CDS             complement(2825..4018)
                     /locus_tag="VKQ27_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HLY89136.1"
                     /translation="MLGAIYIGMSGMDAYEKGLQTISNNVANLNTLGFKATEVSFTDL
                     FDYGGQGEMLGSNAGSWQSGNGVSYGTPFIDFSQGTLNQTSQGLDLAIQGGGFLMLLG
                     GAQPVYARTGSFKVDDQGFISLQGSDRHLAVIGPDGKPEAVNVSAKRTSPPKATTTIT
                     FSDNLSSSATDATVSNLSVYDSNGVKQTWTVKFTRNQSSGVTGNWAVQVTDSTGRTVG
                     TSTLNFIGGTIDPTTTKLVINDTPAGADPLAVTLDFSSGVTSFSSGTTSTIQATAVDG
                     NGPGDLSDVAVDENGQIKLTYSNGKTDLEGAVAVADFRDPQQLQRIGNGIYTHNGTEQ
                     VTLRASNTAGVGTVVSKQLETSNVDLSKEFGDLILVQRGYQASSQVVSVANDMIQQLF
                     GIRGQ"
     gene            complement(4030..4437)
                     /locus_tag="VKQ27_09140"
     CDS             complement(4030..4437)
                     /locus_tag="VKQ27_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HLY89137.1"
                     /translation="MAVAPVSGSSGAQSAYGLDFQSLLKIILTQLTYQDPLKPVDNTE
                     FVSQLAQFSQLQQSQTLNNQITTLLSAQAATQATSLLGRTVDMVATSTTGSTTSGQVT
                     AVSFSSGQPQLTIKTADGQIVSSLSLADVTTVR"
     gene            complement(5455..5904)
                     /locus_tag="VKQ27_09145"
     CDS             complement(5455..5904)
                     /locus_tag="VKQ27_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89138.1"
                     /translation="MGRPSAKSLRVIAELRGIQETAASLAAGAAAARSRSAREALLDS
                     DRRLGELHGGWEASRDGKAIDIEAGLRWSAAWRRAAGDNEHRRDLLLTMEKDERASRS
                     LWEDAMRREDAATSEYRRAQTKEQARIEETRLQEVSERFSRLWATRS"
     gene            complement(5904..7223)
                     /locus_tag="VKQ27_09150"
     CDS             complement(5904..7223)
                     /locus_tag="VKQ27_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HLY89139.1"
                     /translation="MMAHPWQDRIRMADPVRRTGRVRRIRATHVEADGPALPLGAHCR
                     IAGQRSDGPAPILAEVVRVDRESISLVPFEDGASTFPGAEVVALPASQHVAVGDAFLG
                     RAVDALGRAIDEGMNPKGTTLAPLHGETVSPLDRASPRQMLPTGIRAIDGLLTLARGQ
                     RVGIFAAAGVGKTSLITQLAAQVDADVVVLVLVGERGREVEALWSGLPSSIRNRTSLV
                     AATSDQPAMSRVRAGHYAMALAEHWRKQGKQVLLLLDSVTRLAMAMREIGLAAGEPPT
                     VRAYTPNVFAAIPRLIERAGAMRAGGSISAVMTVLSETDDVDDPISEMMKSLLDGHII
                     LSRTLAEQGHFPAIDVSRSVSRQAEALRSPEQAVHVRQVAAWISAYEQSRAMRQAGLY
                     ASGSDALIDEAIARHASITAFLRQGSTEASSPEKSYALLEALQGVHG"
     gene            complement(7220..7885)
                     /locus_tag="VKQ27_09155"
     CDS             complement(7220..7885)
                     /locus_tag="VKQ27_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89140.1"
                     /translation="MSPLIKAGGDLSPPVRAIGDRLARPTGEASAQHRRIEQLEALLR
                     ERDGQLEEMRQSLASQRQEAFDEGFAAGLATADERTQDRLALLGKGIVDAKADLDVAI
                     AGTERLAALLARECLDKMFDEVEDHAHLVCGLIGKQVALMESAALLAVHVSSSDFSSA
                     ALSELQCKFDLEGVEFHSSKDLPSGACRFHLRLGAMELGVDQQWPALQQLLTDLAREH
                     QPA"
     gene            complement(7882..8244)
                     /locus_tag="VKQ27_09160"
     CDS             complement(7882..8244)
                     /locus_tag="VKQ27_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89141.1"
                     /translation="MAFELDRALRREIARLAMLEPDDLRAILEALEPGRRRRVEGLLA
                     QLSEPDMPNPQGTKWPSWIEDRASGRIPGLTDRSRKALAACAERVVPNDAAPRRAAKP
                     QSLVEQAFHALSRSGVRR"
     gene            complement(8228..9454)
                     /gene="fliF"
                     /locus_tag="VKQ27_09165"
     CDS             complement(8228..9454)
                     /gene="fliF"
                     /locus_tag="VKQ27_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HLY89142.1"
                     /translation="MLDRFRSLAVGRQLVIAIGGVVLLTALLVGAWTMLRTPYRPLFT
                     GLKPADAAAIVGELQHKKSAYRLADGGTTVLVPADKVDATRLDLMNGTLALKGTVGFE
                     LFNKSDMGLTDFAQRINYQRALQGELERTIMALDGVDSARVHLSLGEDRIFRQDRVPP
                     KASVTLHMRADASLPAVMATGIQRLVAAAVPQLEPTDVVILDERGEIIGASSAPTGQR
                     STTTDEGQAVGDYYAARIRAALRPSFGDHVQVQAHADLTQTGEQANPFDGAARSFPLQ
                     VTLAADSSVEPAVHEDIQTVTTEAAHLDPARGDVINFGPITRPADVATSPALPMVTKP
                     VATVAPAVSSEEAESASSYYRWSALLVAILAGATIFLLRSRARVRTLSPAQRAKLAAR
                     LDDLLEREEARDGIRA"
     gene            complement(9468..9776)
                     /gene="fliE"
                     /locus_tag="VKQ27_09170"
     CDS             complement(9468..9776)
                     /gene="fliE"
                     /locus_tag="VKQ27_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HLY89143.1"
                     /translation="MPITALPPAAGMLVPQALPVSQAPAVGHGPTSFAHMIEQGVETV
                     NDKVTAADREVRAFVLDDSIPVHQVSFALEQARFSLELMLQVRGRLVEGYQQLMNMQL
                     "
     gene            complement(9782..10210)
                     /gene="flgC"
                     /locus_tag="VKQ27_09175"
     CDS             complement(9782..10210)
                     /gene="flgC"
                     /locus_tag="VKQ27_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HLY89144.1"
                     /translation="MKAMEISRTGLDVEWRRLEVIAENLANAGTARTADGTAFQPREL
                     ISGPKGEFASYLEGHPNPDRLAGVEVYGIEPTNDAPRVVHEPGNPQADAQGMVTYPGV
                     DQAAQMTLLVKTARAYQSNIVAMTAARDMYSKAIDLGGRS"
     gene            complement(10212..10541)
                     /locus_tag="VKQ27_09180"
     CDS             complement(10212..10541)
                     /locus_tag="VKQ27_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89145.1"
                     /translation="MTIKALDGLAQRATVTSENIANSGTRGYRPLRVTFEDALRRAAD
                     QGVDAVQSVTPRTERLPLDNLPAGGELRADLEMSTASTTALRYGALISVLGREMQLDT
                     LAVTGNG"
     gene            complement(10572..11681)
                     /locus_tag="VKQ27_09185"
     CDS             complement(10572..11681)
                     /locus_tag="VKQ27_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HLY89146.1"
                     /translation="MTRLVLLLAALLLPSAAFAETGDVRVKELGRFLGWRENALVGYG
                     IVTGLAGSGDSPHSAATQQALSNVLSRLGTSLPPDVLQSRNVAAVIVTATLPPDANVG
                     DKIDATVTSIGDARSLVGGTLLMTPLLGADRNSYALAQGALVVGGYHFEDNLNLKQKN
                     YPTSGTLSAGATVELPVHAELLGPDRRLTFVLKNPDFTTAERIADGLNRALDRQHATV
                     ASADRIFIQVSDTADANSVITQIEGVTIRPDQLARVVINERSGTVVAGGPVQISSVVI
                     SQGDIKVSVTTDNQASQPYILGGYAPGARSLIVTNSKLEVSDAANDAVIRFPNTTIAD
                     LVMGLNRAKVSTRGIIAILEAIKAAGALQADIIVQ"
     gene            complement(11678..12226)
                     /locus_tag="VKQ27_09190"
     CDS             complement(11678..12226)
                     /locus_tag="VKQ27_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HLY89147.1"
                     /translation="MTALSAHTASADNLYRPGSWPALASDRNARQVGDLVTILVYENA
                     SATNSASSGSAKSAKMQGAVTAGHSFNKSGGVEETGASDSSGTTGRSGQMVAQISATV
                     SAVEPNGDLDIRGEQVIHINGERTLIKIKGRVRTADISTANTVLSTRLADAMIDYDGS
                     GFVSRSARPGIVTRIFSWLGLL"
     gene            complement(12789..13559)
                     /locus_tag="VKQ27_09195"
     CDS             complement(12789..13559)
                     /locus_tag="VKQ27_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HLY89148.1"
                     /translation="MNGAFYIGATGLDTQQRALDVIANNIANLNTPGFRRSTVRFSEL
                     VSVPRDGNDLPIAAADGSAGMSGVQLASSTHVWTAGDLRQTGSSMDVAIDGDGFLEMM
                     GPSGQILLTRGGTLKVNTDGYLAGADGTPLRAMISVPQGTSQLTINPDGTVLAASQGE
                     AAHQIGQIELVMAKDPDALVDRGGGYYEALDDADLTAARPGLDGAGSLAQGAIEQGNV
                     NLSDEMVTLMLVQRAYSASAQVVQAGDQLMAITNGLRR"
     gene            complement(13556..14311)
                     /locus_tag="VKQ27_09200"
     CDS             complement(13556..14311)
                     /locus_tag="VKQ27_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HLY89149.1"
                     /translation="MRRPLPCAFHFINPVFMGVLIAFAEAILSRASGRAEIAAQNISN
                     LTTPGYKSRASFQALLTASPANVRTPDAVGSKIDWSPGKIVQTHNPLDLAISGDGFFA
                     VRSGDKLFYTRNGQFQRDADGHLVTADGMVLQATTGDASVPAGVPAIAPDGTIREDGK
                     DAGQIVVVDFKDKSGLVAMGNGCFAAPTNAAHAIDTPRVVQGAVEQSNASDATEMLSM
                     MASLRSAETGQHIVQLYDDLMAQAATGFGQGQS"
     gene            complement(14349..16400)
                     /locus_tag="VKQ27_09205"
     CDS             complement(14349..16400)
                     /locus_tag="VKQ27_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HLY89150.1"
                     /translation="MSSAVLGRIFGKNHDLVLVFGVIMILLVLFAPIPPALLDLLIIT
                     NFALGLTILLLTFYVARPVSFSTFPSLLLVATLFRLSLNVAATRLILTGANAGEVIGS
                     IGSFAVQGNFVIGLVVFFILVVVQYVVVTSGAQRVSEVAARFVLDSVPGQQMSIDADL
                     NMGLIDQNEAKRRRKELEKEAAFYGAMDGASKFVKGDAIAGIIILLIDVIAGWIVGVA
                     QMDMGWSDALQTFTLLTIGDGIVTQVPALVISVATGIIVTRSSADRQLGTEIFSQLAS
                     VPKIPLIVLGALTLLLTLPGMPKWPIALLMLLGIASFAAARRRKQAEEEEALFDVAQA
                     DAEQAGRAPGVPPVEVALGRELGQQWMAIKPLLAERIATLRAEREKSTGFVLPSVILQ
                     DGPQLASFDYEILLHGARHGTSRIFPDRMLAIRAESATRGPAIGGIETKEPAYGLPAI
                     WIEEQDYDPAREQGYTLVDPVTVLMTHLGEVMRVEAPSLLSRADVVAMLEGVRSRQPG
                     LVEELIPAIMTVSDVQRVLQNLLAEEVSIRSIDRIAEALVDVGRSIKDPAELTELVRQ
                     RLSHAICQSLRGSQDQLSVLSLDPRIEGQIGESLRRTDGSNAMILEPKFADQLIRRMI
                     PLTESMIKQRLAPVLLCSPEIRRHFKTFTRRSLPRLAVLSVSEVPAAIDLASFAIVKI
                     D"
     gene            complement(16397..17464)
                     /locus_tag="VKQ27_09210"
     CDS             complement(16397..17464)
                     /locus_tag="VKQ27_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLY89151.1"
                     /translation="MAEQEQNRSEAPSAYKLMRARRQGQVARGLDLGFFTALCAFLAY
                     AWISGERLISTLARSMRDAFISSAQLAGDRDTIFAVIGLLGSRIEGPVATFATSIFVL
                     VLLLELLQTGIVFSSEPLKLDFSRLNPAQGLKRLFSIRLLLETAKNLLKLALYSIAAW
                     LIVRNMLHSDVASIQDAASLAHVMTHSAMRFLEIFAALALLFAAIDQLIARRGFLKRM
                     RMSRREMKQEVRDREGEPRLKQKRKQMHAEFVRNSRSMKGLRGADMLIVNPLHIAVAL
                     RYDPRSMVAPTVVAVGVNQLALRLKRLAFLYGIPVVEDRVLARSLLRSAAVGHAIPAT
                     SFQPVADIYNRLRRQRRRQEK"
     gene            complement(17464..18186)
                     /locus_tag="VKQ27_09215"
     CDS             complement(17464..18186)
                     /locus_tag="VKQ27_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLY89152.1"
                     /translation="MLLGLRIAPSFALAPPFTLVRMPALFRVLLGLGLSAAILGARPT
                     GALTGDIEPQVLFPAAVRELAIGMMFVLVLQLLFGALYLAGRTIDVQAGFGLAILIDP
                     TTRAQTPLVGTLFAYATAAIFFANDGHLALLRLMAATLDAVPLGSWTMPHALQHLLTF
                     IAVIFSMALGFAGTVIATIFLIDVAISFLSRTVPQMNILVFGFQVKTLTLLLTLPLVF
                     SLGGTLLVRMVTVAIEAIPGMI"
     gene            complement(18227..18493)
                     /locus_tag="VKQ27_09220"
     CDS             complement(18227..18493)
                     /locus_tag="VKQ27_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HLY89153.1"
                     /translation="MNSDRALELMSQLLWNAMLIAGPVLVTALAVGLLVSVLQVATQL
                     QEATLSYVPKLLACAAVLVLLGPWLIGRLTAYATALLMMIPQLS"
     gene            18705..>29979
                     /locus_tag="VKQ27_09225"
     CDS             18705..>29979
                     /locus_tag="VKQ27_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLY89154.1"
                     /translation="MSSKNGGKSGSARDLGVAMVAVFTGLGLGVERGSGFVLGSRGQL
                     GSAGFGRFGENVYVNAATGNLAIQRSDEVLLGVGADANVGRSYNSLGAMSDDNGDNWR
                     LNASRSVAGLTGTLGTVGSTVTRTDWDGSDTVYSWDASRNAYVCQQGSGAYDTLSFAG
                     GIWTWTDGSSQRIETYDDAQGGRILNSVDTDGNALSFSYTGSLLTRVTTADGEHVDMS
                     YAGNNLTQLVTTLQDGSTLSRVRYGYDVQNRLSSVTTDLTPADNSVADGQVVTTTYSY
                     DGTSDRVASISQTGGARLDISYVLVGSDYRVASLTQAQASGASSTTSFSYDVANRITT
                     VTDAQGQVSKLTYDAQGQLVRLEQPAPVSGGTALVSTFSYTATGDVASVTDGNGNQTS
                     YSYDANGNLTLSRDAAGNTVAYSYDAANHLLTETHYLVPDPDGAGAGQPGQPLTTRYA
                     YDAHGHLRYVVSAAGRVTRYDYTASGQVASNVDYATNLYDTSGLGATDPLALATLDSW
                     TAGIADKSGFHVTDTSYDFRGNVSTVTSYSQTSTAGVGLTSAAYSRVTYVYDQAGHLL
                     SRHDNRTGAGSETFLYDGLGRLIGSTDLAGATTSIVFADASNSQVVTAANGVTTTSVY
                     DLAGELISTSLSGTGVAAMTTSYAYDSLGQLRMTTNALGQKSYFLYDHDGREVATIAA
                     DGALTETVYDAGGRVAHTVQYATKLTAAQLASLVDANGKPTSVDLASVRPSANAADRW
                     NWDVYDQANRLIETIDSAGGATVYGYDGASQLVSTTRYANAIAAATLAGFKTTPAVTL
                     QLPVADTAHDRVTRSFYDQDGLLVGTLDATGALTQFVYDAAGEKIRTVGYANFTSADL
                     RATGSFAQLLASVGTSATDNRIDSVYDDEGQLRFTLDANARPTEFVYDASGNLIHSID
                     YAGSIAPAASYALASVQAQVASLAGDPANRTTRFVYDAAGRLAYVIDAAGTVTATSYD
                     VVGHVVKQTVSATSYTAVGDPDLASMQAWASASAGAADHVSRVLYDGVGRPVYAVDAE
                     GYVTESQYDALGEVVKTIRYADRYTVDDTTTTAQLQTLIGTLPASAVVTSYAYDTAGR
                     VTDMTDATSGVTHYGYDALGQLTDTTLAYGSADAVTTHTAYDSAGRVLSVTQGYGTAV
                     AATTSYTYDALGHVLTVTDARSNVTTNGYDALGRVVSVTDPLSGVTSYQYDAFGNRVK
                     TTDVRSNTSYAYYDALDRLVLQVDAAGYATATSYSVGDAVLSVTHYATKVSGSYGVGT
                     PPALTASATKDEVTSFIRDKLDRVTRVTDAEGYYEQYTLDAFGNRGTVRNKLGGITTN
                     VFDRRGLLVSETKPETSTRSDGSIEASSVTTSYAYDARGNRIQMIEAAGLAEQRTTSY
                     AYDKLDRMVSKTGDAFAILNSDKTSTSLATPVETFRYDARGNLIEHDDAAGARTLSYY
                     DALDRKIASVNALGTLSAWTYDGNGNVLTTRVYGDAVALPATPGGTPPAPVNSANYRE
                     TRYAYDKDNRQIETRVVSIDVGEHSNGTYVNGTQDIVTATAYDAAGNIISQTDPRGHA
                     VYSFYDKAGHKIAEVDQENYLTSWDRDGDGNVVTETRYATRLSVVVGTASDPAALKVN
                     AGSSADDRITQFSYDRNGRRLTETRLNLVASSVSATGALTTVTTNATVSYNYNGLGQV
                     TRKTEANGDTTDYSFDFFGRMTEIARSGYVDSNGASVRPTTDMQYDGLDELTRQLVRG
                     TDATTEADDRITSYAYDKAGHVITMTDPTNFVTSSYYDIAGHLVKQQWVRLKSDGSGE
                     TDADFYSYDLLGHQTMQQLGIFNGGTSWTLSETTDMRYDAYGEMVARGISTGGNPANY
                     QEFADYDAMGRVWRTNFNDGVTKAYVYDANGNATLLLQSTGSADLRTLTLAQMVAAGS
                     GVTETQSVFDKRNQLIETIQPSIANAAQLPGSINAFDTVNAGSNFTGGAVSVGSTRGA
                     TGSSGSVSGTPGALAADRGTVTFTLQWAAPHGSGSYTPNFSYVGNIGYGGGAFHLWIS
                     GPQNYNKEVVPGQRSLDGLVNGTYTLTLYQDGPAGTGQVFITQGSIVVSATSGSASIP
                     VSPPANLQFQGQNPNTSKVMLFVRPQGSSGPYQRVATTQLLNSAGAAVAGWFTADPNA
                     APFNGLPANSGWDVKYYAMDANGNTLNAQTATLSVDGSGNPSFSNIVAQPIGGAGKSI
                     LTREFNGDWLISSEQAPGTQSVLMYYRVAGSNSAWSTTWMSNNGYGQHNLWATSVTNW
                     GGPYEYWLEDFSGTNGTGSLIGKNYGTFSVGGQPSALTSYADQPETVHIYNQPPATSM
                     LLWYKPSGTSNWIQAANPTWNGATGSWDWDANGITPDKLNDYAYDFRYETYNGSVLVN
                     QAHGQVQLGYDPAVFSNVIDQLPATVVFSPPQANATTLILNYRAAGSTGAYSTITLSR
                     DASGKFSVNADAARPASGSITYEYFYDLRDSAGNPVAPVGGEDHATGYLTISADRSTD
                     TRTLQWVLVGSLDPAATIDRQQAYNAFGDIVSETDGLNRVTTMAYNVIGKLILKQAPL
                     VSVTDEHGVQSQVRPTERYYYDLAGRLVGVDDANGNRNTLALLAGSGEEAGENAITTK
                     EFHADGGVRSYGIDVFGDVRTVTDELNFVTTNSYDKMDRLVQVAHPTRVGGNSNGVAL
                     TDFYAYDGLGHRIKHWNSQLGSTVVETTDYGGKGRVIQTRDFAGNVTSYSYSWSTTLT
                     NSGTGTVGGLIKSETDASGHTSVEQDDYFSRVVTKTDLGGHSFNYGYDKAGNLIQQTN
                     TAGQSIAYAYYGNGYIRSITDNSLHVQSTFEYDKEGNRTLESYIGLGTNPIYYQVAAI
                     SYDELNRIKTFTDAKATISYEYDANSNRRRVLSYYHDGINGDAQTQDYWYKYDGMNRF
                     VLTMGTLSGGVGGTIQTGTTGVQIGYDAAGQRKFAINGSDGSREDYSYTADGYLEDTK
                     INGVLRARRVNDAVGRVTTYSEYLTDGVTTNFSQASTYDGDNRVTDQIVTQRQSDGSY
                     TYTNVHNDYRADAGGGVYTGADQGVITHSRQQQQGSSVVQDTVYGYQWWDEAKQASIQ
                     VKGTDPNNPNNYQWAPGASQLSYDVNGHLKSAVISGGTAAVVTYTNDAYGQVLEREQT
                     SGSTIGPRQLYYYFDGNRIGDVGNDGPSQVDYAAALAQRGTVVAPGAFRGGKPVASAD
                     FDENYQPIGPSYPGMAATAYTVRSGDTLASIAQAVWGDSSLWYLIADANGLTASDTLV
                     AGQQISIPNKVANIHNRTGVYRVYNPGEAIGDLLPTLPAEPAPPVHNHGGCGVFGQIL
                     LVAVAVAVSAVTYGALGGEALQGVAAIEAGAAAGAAGSVASQALGVATGIQDHFSFKG
                     VALAAIAGGIGSGLGKVGILKNATDFDKVLQNVARGAISNGLTQGVALATGLQNQFDW
                     TGVAAGGIASGVAGAFPGVGGHGVDHYLAEGLSGSAAAVAASAARSLLSGSDFGDNIL
                     RELPDAIGATIGNAAAHTAVAEFASRSGPTAYNTYNGPQDARVISDIVVVGRLQNWAD
                     RLLRGLDAVADWAADHNIVTALADPISRQLDNAGLHSSANLVHAAGTFVASGERAIYH
                     DAVGIGRAIVHPVQTELAIAGALDTAIQSNASDVLNGARQSYNNFASANIDTQAGMLG
                     GAAGHVAFATLSGPVAEASLGRIASLVGDARIASIPLDTRGLAADSAAVRVGPYGDLT
                     AELKGTGIQANHLNQDAAFRDLIPRNDGLAVGMRGNAFTEVGSAHYEFHSSLEEFWSP
                     YR"
BASE COUNT         5782 a         9530 c         9451 g         5216 t
ORIGIN      
        1 gcgctcccga gacggcaagc tctcgatctc tacaaggagc cgctcggtga gttcgtacca
       61 cgccgcactg tcatagccgt tcgcctgatc ccgcgatacg atcgtctcct cctcgtcctc
      121 cacatagagg cgggttccct ccagcatgaa gcctacggca agacccatcg cgatcgctgc
      181 cagcgattga agcgcatcgg cgtcggacag gccgacaacg tcgccgggcg caagtgagcg
      241 caaccgatca cgccgcactc gtgcgcgcgc tgactgctgt tcgcggactt cggacatggt
      301 cgagatgccg ttcgaaatgc tgccggagat acgatgtgca gcataagtgc ggaaggcgac
      361 gccgcgggta ggatcgaagc ggtcgatggc ttccaggagg ccggtgcacg cccattgatg
      421 cacgtcctga aattcgaggt cgccctggga gcggccattg aactgccggc gggcgatcga
      481 gcgggcaaaa ccggcgtggc gttcgaagag atgttgtcgc gccgcgtcat cccgatcgat
      541 gcgcagcgcc gaccataagg cggcctcatc ggcggacatc accccaggag cgagtgcagc
      601 catgccgtca actaatggaa actgcccagg acgccttgca gaatgaggct ccatccgtcg
      661 atcaacacga acatcaagac cttgatcgga agtgacaggg tcgagggagg aaccatcagc
      721 atgccgagcg cgagcaggat gctggagacg acgatatcga tcaggagaaa gggcaggagg
      781 atcacaaagc cgatccgaaa cgctacacgc aactcgttga gcatgaaggc cgggaccaac
      841 tgcatgaagc cgacatcggc tgcccgcgcc ggcaagggtg cgcgcgcgat gtcgtagacg
      901 gccttgatgt cctggtcgtg cacctgcctg agcatgaact cacgaagcgg tgcgccgccc
      961 gcctgggcag cctgatcgac agtcattcgt ccggcgagaa agggctgcag cgcatgatcg
     1021 ttgagcgctt cgaaagtggg cgccatcgag aacatcgtca ggaagatcgc caggctaacg
     1081 agcacctggt tcggtggagt ctcgggcatc cccaatccat ggcggatcat cgagaggacg
     1141 acgatgatcc gtgtgaagga cgtcgtgcaa atgacgagtg caggcaggat ggccatcagg
     1201 gagaggatga gcgcgatcct caccgtctga gccgtctggg gctcgacgcg cgtcagcgcg
     1261 gaattctgcg tcggcgtacc gagcatcgac gattctgcgg cacgcgccgg agccgccgcg
     1321 attgtctgac acggtgtcag ccagccgacg agaaggagtg ccgtcaaaat cgccgtcctg
     1381 actgctcgcg ataatatttg ccgcgtcact gtcggatctc gggaggaaga tgggtgatca
     1441 gttgcgcgcc ggacgccgag gtcgaaatca gcatctcacg gccgtcgcac gcgacgatcg
     1501 caagctgaac ctgagggctc acgcgcagac tctcgtgaag ccgcaggcgc cgcggactct
     1561 tgccaggcag ggtgagcgcc agcccaccac cgagccgagc acggatcgcc agcgcgccgg
     1621 cgaccgccag ccccaggcag aggaggaggg cacccacgac gcgaccgaat gagacgccgt
     1681 catcctgcgg gttggccagt tgagcgaacg cgggcccaga agcgctgagc actgccagag
     1741 caccgaacca ccgcgcatat cggacggggg ctctcattgt ttggctgcga cctcaacgat
     1801 ccgcacgcca aacttgtcgc cgactgcgac gatctcgccg cgcgccacgc acgcgtcgtt
     1861 cacataaaga tcgacatggt ccgcaagccc cgtggacaag gtgacgacgg aacccgattt
     1921 cagcgccatc aacgcatcga cgctgagttc agcgcggccg agccgcgctt cgatatcgac
     1981 acggacatcg cggaaaagct cctgatcgat ggcgacgatc ggcttagcgg cgctcgcatc
     2041 ggcagttgca gggagagggt tgttcttgtc tgtcatgatc gatgggcgaa ccattaaaat
     2101 tggagagtgg gcttgtcgtc ttcgcgcccg agctggccgt gcacgattgg gcggccggag
     2161 cttgacaggg tcatcgccac gcccgcgtcc agcgcctggt cgaggatcaa tatgtccccg
     2221 atcgcgagat gcgtcaggtc ggcgagcgca atcgaggctg atccaacgac cgcatccaac
     2281 gataaagtcg tttcgccaat cgcgcgcgat cgtttggtca gcgtgccctc acgaccccga
     2341 ggctgcggca tgcccgactt cagccacgag gctagcagcg atcgcgagac gttgatcgtt
     2401 aacagatcgc gccccttcag cgtcaacacc aggctgtgcg atggcgtatc atgcacgcga
     2461 ggcacttcca gcatggcgtc gagccttgca agcaagtcac tggcaatctc ttcgaccagt
     2521 atatcgacca gacgacgatc cgtgtctgat agctcgatct cggccaaatc caccgaacag
     2581 gccgtttcga agaggagccg tttgccgcgg gctgtcagcg agatcgtcgt cggttggccg
     2641 ggccggtcga caacgaggcc gtggagccct gtcgctcgat cagcctcgcg gacgtccgcg
     2701 aggctgatca tcgcatcgac gaaccagtca ttggaccatg cttcaagcac cggcgagagc
     2761 actcgccgcg ccgccgaacg ttcgaacgca ccatccggaa gccaggctcg ggcagccgac
     2821 gacattattg ccctcggatc ccaaagagct gctggatcat gtcattggcg acgctgacca
     2881 cctgcgacga cgcctgatag ccgcgctgga cgaggatcaa atcgccgaat tccttcgaga
     2941 gatcgacatt cgaggtctcc aactgcttgg atacaactgt tcccacgcca gcggtgttgc
     3001 tggcgcgcag cgtcacctgt tcggtgccgt tatgggtgta gattccgttg ccgatgcgct
     3061 ggagctgctg gggatcgcgg aagtctgcta ccgcgacggc gccctccaga tcggtcttgc
     3121 cgttggagta agtgagcttg atctgcccat tctcgtcgac cgcgacatcg ctgagatcgc
     3181 cggggccatt gccatccacc gcggttgcct ggatcgtcga cgtggttccg gacgagaagg
     3241 aggtcacgcc agacgagaag tcgagcgtga ccgcaagcgg atccgccccg gccggcgtgt
     3301 cgttgatgac gagtttggtc gtcgtcggat caattgtccc gccgatgaaa ttgagcgtgg
     3361 atgtgccgac cgtgcgtccg gtcgagtcgg taacctgaac cgcccagttt cccgtgacgc
     3421 cggagctctg gttgcgagtg aacttgaccg tccaggtctg cttgacgcca ttgctgtcat
     3481 agaccgacag gttcgatacg gttgcgtcgg tcgccgacga ggacagattg tcggagaatg
     3541 tgatcgttgt cgtcgccttg gggggactgg tccgctttgc tgacacgttg acggcttccg
     3601 gtttcccgtc gggaccgatc accgcgaggt ggcgatcgct tccctggagc gagataaagc
     3661 cttggtcgtc gaccttgaac gaacccgtcc tcgcatagac cggctgcgcg ccgccgagca
     3721 gcatgaggaa acctccgccc tgtatcgcga ggtcaagacc ctggctcgtc tgattgagtg
     3781 ttccttgcga gaaatcgatg aacggcgttc catagcttac gccattaccc gattgccaac
     3841 tgcccgcgtt ggaaccaagc atttcgcctt gcccgccata atcgaacagg tcagtgaagc
     3901 tgacctcggt ggccttgaag cccaacgtgt tgagattggc gacgttgttg ctgatcgtct
     3961 ggaggccttt ctcataggcg tccatgccgc tcatgccgat atagattgct ccaagcatgc
     4021 ccgttcctcc tatcgaaccg tggtaacatc ggcgagcgaa agactggaaa cgatctggcc
     4081 gtccgctgtt ttgattgtca gctggggctg acccgacgag aaactgaccg ccgtcacctg
     4141 acctgatgtc gtcgagccgg tcgtgctggt tgcgaccata tcgaccgtcc ggcccagcag
     4201 gctcgtcgct tgtgttgcag cctgcgctga aagaagcgtc gtgatctgat tattgagcgt
     4261 ctggctctgc tgcagctggg aaaattgcgc aagctgcgac acgaattcgg tattgtcgac
     4321 aggcttgagc ggatcctgat aggtcagctg cgtcaggatg atcttgagca aggactggaa
     4381 gtccaggccg taggcgctct gtgcgccgga tgagccacta actggcgcga cggccatggg
     4441 atactccttg ctcgaaggcg tggtcgggca ccggctgacc attcaaccgg acatcatgga
     4501 tcgaaaatcc gaactcggcc gcgacatcgg cgagcaatga ccgaagcttg gcgcggccct
     4561 cgataccatc ctcgccgccg cgggccacga cctgcaagga accggcttgt tcggacacgg
     4621 tcaggctgac cgctgtgggc tggggatcgg gcgtggctgc ccatcgtccc tgacgcgggg
     4681 tggcggccga ttgcgtcgct ggggcgtcga cttcggtttc ctcggacaga ggagatgcca
     4741 aaggatttgg gaggcttgtc acgggggcat cttgcaccgc tcgtacggga cgaagcgagg
     4801 caagagcagg caagaccggc cccatcattc ccgcgggatc cgggacagtg acaacaggag
     4861 cggctcgccc gtcccgttcg tcgagagcgg tgcttcctcc gatgagcgtg accgggggca
     4921 tgccgtcggg ggcgagtgcg cgccaagaag caggcgcgtg tccgtcaccg agctgggctg
     4981 cgccaaatct gccgaacacg ccgagcgcgc cgaaagattg ggtaggttgc gcagttgctt
     5041 cctgctcgtc cgaccgatcg gccagcgcgt cgaagggagc cgtgggactg gcgatcataa
     5101 ggggcggagg aacctggtgg cttccggctt gtgaaacagg cggcaaggaa agccctgggt
     5161 gaggcagtcc tttgggcaaa gaatcgatga tgcccgtgat cttgtagtcc gaagacgtcg
     5221 gaagcgctcg ctcacctcct gaattcgcct gtcctcgatg cgggcctgct ccttggatta
     5281 gacccggcgc cgccgctgca cgcggctcat ccgatcgatc gagcagcgcg tcgaaggggg
     5341 gtgtgggctt cgcgtacgta ggagccgtcg ggacacgatg gcttctggct cgaggagcag
     5401 acggcaagga gagcccggga tgagcccgtc cttcgggcaa ggaaccgatc atgctcatga
     5461 ccttgtggcc caaagacgcg agaagcgctc gctcacctcc tgaagtcgcg tctcctcgat
     5521 gcgggcctgt tccttggttt gcgcccggcg atattcggat gtcgcggcgt cctctcgacg
     5581 catggcatcc tcccacaggc ttcgactggc cctttcatcc ttctccatcg tcagcaacag
     5641 gtcgcgacga tgctcattgt ccccagctgc gcgccgccac gcggcggacc agcgcaggcc
     5701 cgcctcgata tcgatcgctt tgccgtcgcg cgaggcctcc catccgccat gaagttcgcc
     5761 gagccggcga tcgctgtcca aaagcgcctc ccgcgcgctc cggctgcggg ccgcagccgc
     5821 gcctgccgcg agcgaggcgg cagtctcctg gatgccacgc agctcggcga tgacgcgaag
     5881 tgattttgcc gaagggcggc ccatcagccg tggactccct gcagggcttc cagaagcgca
     5941 tagcttttct cggggctcga ggcctcggtc gagccttggc gcaggaaggc tgtgatgctc
     6001 gcatgccggg caatggcctc atcgatcagc gcatcgctac ctgaagcata gaggccggct
     6061 tggcgcattg cgcgcgactg ctcataagcg ctgatccagg ccgcaacctg ccgcacatgg
     6121 acggcctgct cgggcgagcg aagcgcctcc gcctggcggc tgacgctgcg gctcacgtcg
     6181 atcgccggaa aatgcccctg ctcggccagg gtccgcgaga ggatgatgtg cccgtcgagc
     6241 agggacttca tcatctcgct gatcggatcg tcgacatcgt cggtttcact cagcaccgtc
     6301 atgaccgcgc tgatcgaacc acccgcgcgc atcgctccgg cccgttcgat aagacgcggg
     6361 atcgcggcaa acacgttggg tgtatatgcg cgaacggtcg gcggttcgcc cgccgccagt
     6421 ccgatctcgc gcattgccat cgccagcctc gtgacggaat cgaggagcag caacacctgc
     6481 ttgccttgct tgcgccaatg ttcggcgagc gccatcgcgt aatggcctgc gcgaacgcgt
     6541 gacatggccg gctgatccga ggttgctgcg accagcgagg tccggtttct gatcgagctt
     6601 ggcagtccgg accaaagcgc ttcgacttcg cgtcctcgtt cgccgaccag cacgaggacg
     6661 accacatccg catccacctg cgctgccaac tgcgtgatga gactggtctt gccaacgccg
     6721 gctgccgcga aaatcccgac ccgctggccc ctcgccaagg ttagcaaacc gtcgatggcc
     6781 ctgatacccg tggggagcat ctggcgcggc gatgcccgat caagcggcga cacagtctcg
     6841 ccatggagag gcgccagggt tgtacctttg gggttcatgc cttcgtcgat cgctcgtccc
     6901 aacgcgtcga ccgcgcggcc gagaaatgcg tcgccaaccg cgacatgctg gcttgccggg
     6961 agtgccacga cctcggcacc aggaaaggtc gaagctccgt cctcgaacgg gacgaggctg
     7021 atgctttcgc ggtcgacgcg gactacctcg gcgaggatcg gcgccgggcc gtcgcttctt
     7081 tggcccgcaa tccggcagtg cgccccgagc ggcaaggcgg ggccatcggc ttcgacatgg
     7141 gtggcacgga tgcggcggac gcgtccggtg cgccgcaccg ggtcggccat gcggatgcga
     7201 tcctgccagg gatgcgccat catgccggtt ggtgctcccg cgcgagatcg gtcagaagtt
     7261 gttgcagcgc cggccattgt tggtcgacgc cgagttccat ggctcccagc ctcaggtgga
     7321 aacgacatgc gcccgaaggc aggtccttcg acgaatgaaa ttcgaccccc tccaggtcga
     7381 atttgcattg gagttcgctt aacgcagcgc tcgagaagtc gctggacgag acatgcaccg
     7441 ccagaagcgc tgcactctcc atcaatgcga cctgcttgcc gatcaggccg cagaccagat
     7501 gggcatggtc ctcgacctcg tcgaacatct tgtcgagaca ttcgcgcgcg aggagggccg
     7561 caagtcgctc ggtgccggcg atcgcgacgt cgagatcggc cttcgcgtca acgataccct
     7621 tgccaagcag tgccagacga tcctgagtgc gttcatcggc ggtggccagg cccgccgcga
     7681 aaccttcgtc gaacgcttct tggcgttggg atgcgaggct ttgcctcatc tcttccaact
     7741 ggccgtcgcg ctcccgcagc aacgcttcga gctgctcgat ccgcctgtgt tgggccgatg
     7801 cctctccagt cggtctggcc agacgatctc caatcgcccg aacgggcggc gacaaatcgc
     7861 cccctgcctt gatcagaggg ctcatcgccg cacgcccgat cgggacaagg cgtggaatgc
     7921 ctgttcaacc aggctctgcg gtttcgctgc gcgccgaggc gcagcatcgt tcggaacgac
     7981 gcgctccgcg cacgccgcca gggcctttcg agatcgatcc gtcagccccg gtatgcgtcc
     8041 cgacgctcgg tcctcgatcc acgatggcca cttggtgccc tgagggttcg gcatgtcggg
     8101 ttccgagagc tgtgcgagca gtccctcgac acgccgacgt cggccaggtt cgagcgcctc
     8161 gagtatggca cgcaaatcgt ccggctccag catcgcgagc cgcgcgatct cccgacgcaa
     8221 agcgcgatca agctcgaatg ccatcgcgcg cctcctcgcg ttcgagcaga tcgtccaggc
     8281 gtgcggcaag ctttgcccgc tgtgcagggg agagagtgcg gacccgcgcg cgtgaacgca
     8341 gtaggaatat cgtagcgccg gcaaggatgg cgacgaggag ggcgctccac cggtaatagg
     8401 acgatgcgct ctccgcctcc tcgctgctca cggcaggggc gaccgtcgcg accggcttgg
     8461 taaccatggg cagggcggga gacgttgcga catctgccgg gcgtgttatg gggccgaaat
     8521 tgattacatc gccacgggcc ggatcgaggt gagcagcctc ggtcgtcacc gtctggatgt
     8581 cctcatggac cgccggctcc acagaagagt ccgcagccag cgtcacctga agcggaaagg
     8641 atctagcagc gccatcgaat ggattggcct gttcgccagt ctgcgtgagg tcggcatggg
     8701 cttgaacctg gacatgatct ccgaagctcg ggcgaagtgc cgcgcggatc cgagcggcat
     8761 aatagtcgcc cacggcctga ccttcatcgg tggtggtgga gcgctgcccg gtcggagcgc
     8821 tgctcgcgcc gatgatctcg ccacgttcgt cgaggatgac cacgtcagtg ggctcgagct
     8881 gtggaaccgc ggctgcgacg agacgctgaa tgcccgtcgc catcactgct ggaaggctcg
     8941 catccgcgcg catgtgcagg gtcaccgacg cttttggcgg aacccgatcc tggcggaaga
     9001 tacgatcctc accgagagac agatgaacac gtgccgagtc caccccgtcg agggccatga
     9061 tggtgcgttc caactcgccc tgcaaagccc gctgataatt gatgcgctgg gcaaaatcgg
     9121 tcagccccat gtccgatttg ttgaacagct cgaagccgac cgtgcccttg agcgcgagcg
     9181 tgccgttcat gagatcaagg cgcgtcgcgt cgaccttgtc ggcagggacg agcacggttg
     9241 tgccaccgtc ggccaagcga taagctgatt ttttatgttg cagctcgccg acgatcgccg
     9301 ccgcatctgc cggcttcagt ccggtaaaaa ggggccgata gggcgttcgc agcatcgtcc
     9361 aggcaccgac cagcagcgcc gtcagcagca caacgccacc gatcgcgatg acgagctgtc
     9421 gtccaactgc aaggctgcgg aagcggtcaa gcacggatct tacgccttca aagctgcatg
     9481 ttcatcagct gctggtaacc ctcgaccaga cggcctcgaa cctgcagcat gagttcaaga
     9541 gagaagcgag cctgctcgag cgcgaaactc acctgatgga cgggaatgga atcgtcgagc
     9601 acgaaagcgc ggacctcgcg gtccgccgcc gtgactttgt cgttcacggt ctcgacgcct
     9661 tgctcgatca tgtgcgcgaa tgaggtagga ccgtggccaa cagcgggggc ctgcgaaacc
     9721 ggaagcgcct gcggcaccaa catccccgct gccggcggca gcgcagtgat cggcatcacg
     9781 ctcacgaccg gcctcccaga tcgatcgcct tgctgtacat atcgcgcgcg gcggtcatcg
     9841 ccacgatgtt ggactgatag gccctcgcgg tcttcaccag gagcgtcatc tgcgccgcct
     9901 ggtcgacgcc cggataggtc accatacctt gggcatcggc ctgcggattg ccaggctcat
     9961 gcaccacccg cggcgcatcg ttggtcggct cgatgccata gacctcaacg cctgcgagcc
    10021 gatcaggatt ggggtggccc tccaggtagg aggcgaactc tcccttgggc cccgagatga
    10081 gttcgcgagg ctgaaacgcg gtaccatccg cagtgcgcgc tgtacccgca ttggcgagat
    10141 tctctgcgat cacttcgagg cgacgccatt caacgtcgag gccggtgcgg ctgatttcca
    10201 tggccttcat cttatccgtt ccccgtcacg gccagagtgt cgagctgcat ttcgcggccg
    10261 agcaccgaga tcaaggcgcc gtagcgaagc gctgtcgtcg atgccgtgct catctcgaga
    10321 tcggccctga gttcgccgcc ggccggcaga ttgtcgagag gtaggcgctc cgttcggggt
    10381 gtgaccgatt ggaccgcgtc aacgccctga tcggccgcgc ggcgcagcgc atcctcgaat
    10441 gtcacgcgca atgggcgata gccccgggtt ccggaattag cgatattctc cgatgtgacg
    10501 gtggcgcgct gggcgaggcc gtccagcgcc ttgatcgtca atattgcatt gctgacgtcc
    10561 atcggcggct cctattgaac gatgatatcg gcttgcagcg cgccggctgc cttgatcgcc
    10621 tcgagaatgg cgatgatgcc gcgcgtgctg accttggcgc gattgagccc catgacgaga
    10681 tcggcgatag ttgtattggg gaagcggatc actgcgtcat tggcagcatc gctgacctca
    10741 agcttgctgt tcgtgacgat cagactgcgc gcacccggcg cgtagccgcc caggatgtag
    10801 ggttgcgacg cctgattgtc ggtcgtcacc gataccttga tatcgccttg tgaaatgacg
    10861 acgcttgaga tctgaaccgg accgccggcc acgacggttc ccgatcgctc gttgatgacg
    10921 acccgtgcca gctggtcggg tcggatcgtc acgccctcga tttgggtgat cacgctattg
    10981 gcatcggccg tgtcggagac ttggatgaag atcctgtccg cgcttgcaac cgtcgcgtgc
    11041 tgccggtcga gcgcgcgatt gagaccatcg gcgatccgtt cggcggtggt gaagtcagga
    11101 ttcttcaaga cgaaggtgag ccttctgtcg ggccccagca actcggcatg gacgggcagt
    11161 tcgaccgtcg cgcctgccga caaggtgcca gacgtcggat agtttttctg cttgaggttg
    11221 agattgtcct cgaaatgata gccgccgaca accagcgcgc cctgcgccag ggcgtagcta
    11281 ttccggtccg cgcccagcag cggcgtcatc aaaagggtgc cgccgacgag gctgcgggcg
    11341 tctccgatcg atgtcaccgt cgcgtcgatc ttgtctccga cattggcgtc gggcggaagc
    11401 gtcgcagtca cgataaccgc tgcgacattg cggctttgca aaacgtccgg cggcaggctg
    11461 gttcccagcc ggctgagcac attgctcagc gcctgctggg tggcggcgct gtggggggaa
    11521 tcgcctgaac ccgcaagacc ggtcacgatc ccgtatccga ccagcgcatt ttcgcgccac
    11581 ccgagaaacc gtccgagttc cttcactcgg acgtcgccgg tttccgcgaa cgccgcggaa
    11641 gggaggagaa gagcggcgag cagcaaaacc aggcgcgtca tagcaagccc agccagctga
    11701 agatccgggt aacgatgccc ggccgagcgc tacgcgagac gaagccgctg ccgtcataat
    11761 cgatcatggc gtcggcgagc ctcgtggaaa gaaccgtgtt cgccgtcgag atgtcggcgg
    11821 tcctcacgcg gcccttgatt ttgatgaggg tccgctcgcc gttgatgtga atgacctgct
    11881 ccccgcgaat gtcgaggtcg ccattgggct cgactgcact gaccgtagcg ctgatctggg
    11941 ccaccatctg tcccgagcgg cccgtcgtgc cgctgctgtc cgacgccccc gtttcttcca
    12001 ctccacccga cttgttgaaa gaatgcccgg ccgtcaccgc gccttgcatc ttggcgctct
    12061 tggccgatcc gctgcttgcg ctgttcgtgg cggacgcgtt ttcatagacc aggatagtga
    12121 cgagatcgcc gacctggcgc gcgttacgat ccgacgcgag cgcgggccag gatccgggcc
    12181 ggtagagatt gtcggcgctc gcggtgtggg cgctcagcgc tgtcactgct actcccacga
    12241 gcagccatcg tgcagaccgg ctcattggcc ggcctccgcg tataggacgg acagggtctt
    12301 gccatctgcg tcgcggacaa agagccgttg tcctgggcgg gctggctgca atgcttcgac
    12361 ccgacgctcg atccggaccg cgccgatcgc gacctccaag ttgacccggt catgggactg
    12421 aacgcttggg cccgattgcc atgccgcacg aggcataggc ttctcgctcg ccgacaattg
    12481 agtgcgagcc gcatcgatat gcacgcggat ctcaccgcct gttgatcgcg gcagccagca
    12541 tccgagtacg ggcatcctcg cgcgtaaacc agccacgagc ggggcaaggg ggatcgtctc
    12601 gggtcgtgat cgcgcgggag ccacgaccat attgcttgcc cgcgcccgca gatgtgccgg
    12661 aacggtggag agatccgcaa tatcggccag acggacgggg tgctcttcga tcatcacatc
    12721 ggtgtgaaac gcgatcggtg cgggctgcaa agaacaggca gtatcatggg gaagaggggc
    12781 cggaaggatc aacggcgcag gccgtttgtg atggccatga gctgatcgcc tgcttgcacg
    12841 acctgcgcgc tggccgaata agctcgctgg acgagcatca gcgtcaccat ctcatccgac
    12901 aaattgacat tgccctgctc gattgcgccc tgcgccaggc tgccggcacc gtccaggccc
    12961 gggcgagcgg ctgtgagatc cgcatcatcg agcgcttcat aatagccgcc gccacgatcg
    13021 accaacgcgt cggggtcctt ggccatcaca agctcgatct gaccaatctg atgtgccgct
    13081 tcgccctgcg aggctgcgag cacggtcccg tcaggattga tcgttagctg cgaggtgccc
    13141 tgcggtacgg aaatcatggc tcggagtggt gtgccatccg caccggccag atagccgtcg
    13201 gtgttgacct taagtgttcc gccgcgtgtg agcaggatct gacccgacgg ccccatcatc
    13261 tcgaggaagc cgtcgccatc gattgcgaca tccatgctcg agccggtctg gcgcaggtca
    13321 cccgcggtcc agacatgcgt ggacgacgca agctggaccc cggacatgcc tgccgaacca
    13381 tcggctgcgg caatcgggag atcgttgcca tcgcgaggaa ccgacacgag ttcgctgaac
    13441 cgcacggtcg agcggcgaaa gccgggtgtg ttcagattgg cgatgttgtt ggcgatcaca
    13501 tcgagcgcac gctgttgcgt atcgaggccg gtcgctccga tatagaatgc tccgttcatg
    13561 attggccctg tccaaatccc gtcgcggctt gcgccatcag gtcgtcataa agctgcacga
    13621 tatgctggcc ggtctccgca cttctgagcg acgccatcat gctgagcatc tcggtcgcgt
    13681 cggacgcgtt cgattgctcg accgcgcctt gaacgacgcg cggcgtatcg attgcgtgcg
    13741 cggcattcgt gggcgcggcg aagcagccat tgcccatcgc aaccagacca gacttgtcct
    13801 tgaagtcgac cacgacgatc tggccggcgt ccttgccgtc ctcgcggatc gtgccgtcag
    13861 gggcgatcgc gggaactccg gctggaacgg atgcgtcgcc cgttgttgcc tggagcacca
    13921 tgccatcggc ggtcacaaga tgaccgtccg catcgcgctg gaattggcca ttgcgcgtat
    13981 agaacagctt gtcacccgac cggaccgcga agaatccgtc gcccgaaatc gcgaggtcga
    14041 gaggattgtg cgtctggaca atcttgccag gcgaccagtc gatcttgctc ccgacggcgt
    14101 cgggggttcg cacatttgcc ggacttgccg tcaaaagcgc ctgaaacgac gctcggcttt
    14161 tgtagcccgg agtcgtgagg ttggaaatat tctgtgcggc aatttctgcg cggccagaag
    14221 cacgcgataa aatggcctca gcgaacgcta taagtacccc catgaagact ggattaataa
    14281 agtgaaaggc gcatggcaac ggtcgtcgca atcaattcga ctgcagccgg tcgatccgca
    14341 ggggataact agtcgatctt gacgatggcg aaggaggcaa ggtcgatcgc ggccggaacc
    14401 tcgctgaccg aaagaacagc caggcgagga aggcttcgcc tggtgaaggt tttgaaatga
    14461 cgccggattt ctggactgca cagcagaacc ggggccagcc tctgcttgat catcgattcg
    14521 gtaagtggga tcatgcggcg aatgagctga tcggcgaatt tcggctcgag gatcatcgca
    14581 ttgcttccat ccgtgcgccg gaggctttcg ccgatctgcc cttcgattcg tgggtcgaga
    14641 ctcagcacgg agagctggtc ctggctgccc cggagagact gacagatcgc gtggctgagg
    14701 cgctggcgga ccagttcggt aagctcggcc ggatccttga tggaacggcc gacgtcgacg
    14761 agcgcctccg cgatccgatc gatcgagcgg atcgacactt cctcggcgag aaggttttga
    14821 agcacgcgct gaacgtcgga caccgtcatg atggccggga tcagttcctc gacgaggccc
    14881 ggctggcgcg atcgcacgcc ttcgagcatc gcgacgacat cggcgcgcga gagcagcgag
    14941 ggggcttcaa cccgcatcac ctcaccaaga tgcgtcatga gcaccgttac gggatcgaca
    15001 agcgtgtagc cttgctcgcg tgccggatca taatcctgct cctcgatcca gatggccggc
    15061 agcccgtatg caggctcctt ggtctcgatc ccgccgatgg caggtccccg cgtcgcactc
    15121 tccgcgcgga tcgccagcat acggtcgggg aagatgcgtg atgtcccatg gcgcgcgcca
    15181 tggagcagga tttcatagtc gaaggacgca agctgcggtc cgtcctggag gatcactgag
    15241 ggaagtacaa agcccgtgct cttctcgcgt tcggcgcgca gtgttgcaat ccgctcggcg
    15301 agaagcggct tgatcgccat ccactgctga ccgagttcgc ggcccagcgc cacctcgaca
    15361 gggggaacgc ctggcgcgcg accggcctgc tcggcatccg cttgcgcgac gtcgaacagc
    15421 gcttcttcct cctcggcctg cttgcgtcgg cgcgccgccg cgaacgacgc aatgcccagc
    15481 agcatgagca gcgcgatcgg ccatttgggc atgccgggaa gtgtaaggag caatgtcagc
    15541 gcgccgagca cgatcaacgg gatcttgggc accgaggcca actggctgaa aatctcggtg
    15601 ccgagctggc ggtcggcgga cgagcgcgtg acgatgatgc cggtcgccac tgatatcacc
    15661 agcgcgggca cttgggtgac gattccgtcg ccaatggtga gcagcgtaaa ggtctgcagc
    15721 gcatcgctcc agcccatgtc catctgggcg acgccgacga tccagccggc gatgacgtcg
    15781 atcagcagga tgatgatgcc ggcgatcgcg tcgcccttca cgaatttgct ggcgccatcc
    15841 atggccccgt aaaaggctgc ttccttctca agctccttgc gtcgcctctt ggcttcgttc
    15901 tgatcaatca gacccatgtt gagatcggca tcgatgctca tctgctgacc tggaaccgaa
    15961 tccagcacga agcgggcggc gacctcggac acgcgctgcg cgccggacgt caccacgaca
    16021 tattgcacga ccaccaggat gaagaagacc accagcccga tgacgaaatt gccttgaacg
    16081 gcaaaggacc cgatcgaacc gatcacctcg ccggcgttgg cgcccgtcaa gatcagtcgc
    16141 gtggcggcga cattcagcga aaggcggaac aacgttgcca caagaaggag cgaagggaag
    16201 gtcgagaacg aaaccggacg cgccacgtag aaggtcagca gcaggatcgt cagacccagc
    16261 gcgaaattgg tgatgatcag gaggtccagc agcgccggtg gaatcggtgc aaacaggacg
    16321 agcaggatca tgatgacgcc gaaaaccagc accagatcgt gatttttgcc gaagatccgc
    16381 cccaacaccg cgctactcac ttttcctgtc gcctgcgctg gcggcgcagt ctgttgtaaa
    16441 tgtcggcaac cggctgaaag ctggtcgccg ggatggcgtg tccgaccgca gcgcttcgca
    16501 gcagggaacg cgcgagcacg cggtcctcga cgaccgggat cccgtaaagg aaggcaagcc
    16561 gcttgagccg tagcgcaagc tggttgacgc ccacggccac gacggtgggt gccaccatcg
    16621 accgtggatc gtagcgtaac gcgacggcga tgtgcagagg gttgacgatc agcatgtcgg
    16681 ccccacgcaa ccccttcatg ctgcggctgt tacggacgaa ctcggcatgc atttgcttgc
    16741 gcttttgctt gagacgtggc tcgccctcgc ggtcgcgcac ctcctgcttc atctcacgcc
    16801 ggctcattcg catgcgcttg aggaagccgc gccgcgcgat cagttggtcg atcgcggcga
    16861 acagcagcgc aagtgctgca aaaatctcca gaaagcgcat ggccgagtgc gtcatcacgt
    16921 gcgcgaggct tgcggcatcc tggatgctgg cgacgtccga atgcagcatg ttccggacga
    16981 tcagccaggc tgcgatggaa tagagagcga gcttcaggag attcttggca gtctcgagga
    17041 gcagcctgat cgagaagagg cgtttcagcc cttgggcggg gttgaggcgc gagaaatcga
    17101 gcttgagcgg ttcggacgag aacacgatcc ccgtctgcaa aagctccagg agcaggacga
    17161 gcacgaagat gctggttgca aaagtcgcaa ccggcccctc gatcctgctg cctagcagcc
    17221 cgatgaccgc gaagatcgta tcgcggtctc cggcgagttg cgcgctgctg ataaaggcat
    17281 cgcgcatcga acgcgccagc gtggagatga gcctttcgcc ggaaatccaa gcataagcga
    17341 gaaatgcgca aagcgccgtg aaaaagccga gatccagtcc gcgcgcgacc tggccttgtc
    17401 ggcgtgcccg catgagcttg taggcggatg gcgcctccga tcggttctgc tcctgctcgg
    17461 ccatcagatc atcccgggga tggcttcgat cgcgactgtc accatgcgta cgagaagcgt
    17521 tccgcccagg ctgaacacca gcggcaaggt cagcagtaaa gtgagcgtct tcacctggaa
    17581 gccgaagacg agaatgttca tctggggtac ggtgcgagac aggaacgaga tggcgacgtc
    17641 gataaggaat atcgtggcga taaccgtgcc agcgaaaccg agcgccatcg agaaaatgac
    17701 ggcaatgaac gtcaggagat gctgcagcgc gtgcggcatt gtccagctgc cgagcggtac
    17761 cgcgtcgagc gtcgccgcca tcaaccgaag cagcgcgaga tgtccatcgt tcgcgaagaa
    17821 gatggccgct gtcgcgtagg cgaagagtgt gccgacaagc ggggtttgcg cgcgcgtggt
    17881 ggggtcgatc aggatggcca gcccgaagcc cgcttgcacg tcgattgtgc gtccggccag
    17941 atagagggcc ccaaacagca gttgcagcac cagcacgaac atcatgccaa tggccagttc
    18001 ccggacagct gcaggaaaga ggacctgagg ctcgatgtcg ccggtcaggg caccggtcgg
    18061 acgtgctccc aaaattgccg ccgacaaccc caggcccagc agcacgcgaa agagcgccgg
    18121 catgcgcacg agcgtgaaag gcggcgcgag tgcgaaggat ggagcaattc gcaggcccag
    18181 cagcaaggtg gaggcgatcc acgcggacag gccgagacct ggcatgtcag ctcagctgcg
    18241 ggatcatcat gaggagggcg gtcgcataag cggtgaggcg cccgatcagc caagggccca
    18301 gcaggacgag caccgccgcg caggcgagca gcttgggcac atagctcagc gtcgcttcct
    18361 gaagctgcgt tgcgacctgc agtacgctga caagcaagcc cacggcgagc gcggtgacga
    18421 gcaccggtcc cgcaatgagc atggcattcc atagaagctg actcatcagc tccaaagcgc
    18481 gatcgctgtt cacgaatctc tagacccctc gggctcgcat atccggttgg tcggcagatt
    18541 caaacgcatg atcgcagtcg gactgtccgg aaacggtgtg tcgaaaggcc ccgcaccgga
    18601 tgctatgccc gcatcgcatt gctcgtgccc ggccaagctc gctaagcccg tcttccaatc
    18661 tcattttcaa aaataaatcg ctaaaccaac gccgaaccct ccggatgtct agcaagaacg
    18721 gcggaaagag tgggtccgcc cgcgatctgg gggtggcgat ggtagcggtg tttacgggcc
    18781 tgggcctggg cgtggagcgg ggttcgggtt tcgtgctggg gtcgcggggc cagctggggt
    18841 cggcggggtt cggccggttc ggcgagaatg tctacgtcaa tgcggcgacg ggcaatctgg
    18901 cgatccagcg cagcgacgag gtgctgctgg gggtgggcgc ggacgcgaac gtcgggcgca
    18961 gctacaacag cctcggcgcg atgtcggacg acaatggcga caattggcgg ttgaacgcga
    19021 gccgcagcgt ggccggtctg accggtacgc tgggcacggt gggcagcacg gtgacgcgga
    19081 ccgactggga cgggtcggac acggtctaca gctgggatgc gagccgcaat gcctatgtct
    19141 gccagcaggg cagcggcgct tatgatacgc tgagctttgc cggtggcatc tggacctgga
    19201 ccgacggcag cagccagcgg atcgagacct atgacgatgc ccagggcggc cgcattctca
    19261 acagcgtcga taccgacggc aatgcgctga gcttcagcta taccggcagc ctgctgaccc
    19321 gcgtcaccac cgcggacggc gaacatgtcg acatgagcta tgccggcaac aacctcaccc
    19381 agctggtcac gacactgcag gacggttcga cgctgagccg ggtgcgctat ggctatgacg
    19441 tgcagaaccg gttgagctcg gtcactaccg acctcacacc tgccgataat tcggtcgccg
    19501 acggccaggt ggtgaccacg acctacagtt atgacgggac cagcgaccgg gttgcctcga
    19561 tcagccagac cggcggcgcc cggctcgaca tcagctatgt gctggtcggg tccgactatc
    19621 gggtggcgag cctcacccag gcgcaggcct cgggggcgag ttcgaccacg agcttctctt
    19681 acgacgtcgc caaccgcatc acgacggtga ccgacgcgca gggccaggtc agcaagctca
    19741 cttatgatgc gcagggccag cttgtccgcc tcgagcaacc ggcgccggtt tcgggcggga
    19801 cggcactggt ctcgaccttc agctacaccg cgaccggcga tgtcgcgagc gtgaccgacg
    19861 gcaatggcaa ccagaccagc tacagctatg acgccaacgg caatctgacg ctgagccgcg
    19921 atgccgccgg caacacggtg gcctacagct atgacgccgc caaccatctg ctgaccgaga
    19981 cgcactatct cgtgcccgac cccgacgggg ccggtgccgg ccagcccggc cagccgctga
    20041 cgacgcgcta tgcttatgac gcgcacggcc atctacgcta tgtggtcagt gccgcagggc
    20101 gggtgacgcg ctatgattac acggcgagcg gccaggtcgc gagcaacgtc gactatgcga
    20161 ccaacctcta cgacaccagc gggctgggtg cgaccgatcc gctggcgctg gcgacgctcg
    20221 acagctggac ggctggcatc gccgacaaga gcggcttcca cgtcaccgac accagctatg
    20281 atttccgcgg caatgtttcg accgtcacca gctattcgca gacctcgacc gccggcgtcg
    20341 ggctgaccag cgcggcctac agtcgggtga cctatgtcta cgaccaggcg ggccatttgc
    20401 tcagccggca cgacaaccgg acaggggcgg gcagcgagac cttcctctat gacgggctcg
    20461 gccgtctgat cggctcgacc gacttggccg gtgccaccac cagcatcgtc tttgccgacg
    20521 ccagcaacag ccaggtggtg acggccgcca acggcgtcac caccacctcg gtctatgacc
    20581 tagcgggtga actgatctcg accagcctgt cggggaccgg cgttgcggcg atgacgacca
    20641 gctacgccta tgacagcctc ggccagctgc gcatgacgac caatgcactc ggccagaaga
    20701 gctatttcct ctacgaccat gatggccgcg aggtcgccac catcgccgcc gacggtgcgc
    20761 tgaccgagac ggtctacgac gcgggcggcc gggtggcgca caccgtccaa tatgcgacca
    20821 agctgacggc ggcccaactc gccagcctgg tcgacgccaa tggcaagccg accagcgtag
    20881 acttagcgag cgtgcgcccg agcgcgaacg ccgccgaccg ctggaactgg gacgtctacg
    20941 accaggccaa tcgtctcatc gagacgatcg attccgccgg cggcgcgacg gtctatggct
    21001 atgacggtgc atcgcagctg gtctcgacca cgcgttatgc caatgccatt gcggcggcga
    21061 cgctggcagg gttcaagacc acgccggctg tgacgctgca attgccggtg gcggatacgg
    21121 cgcacgaccg ggtgacgcgg agcttctacg accaggacgg gctgctggtt ggcacgctcg
    21181 atgcgaccgg tgcgctcacc cagttcgtct atgacgcggc cggcgagaag atccgtaccg
    21241 tcggttatgc caacttcacc agtgcagacc tgcgcgccac cggcagcttc gcccagctgc
    21301 tcgctagcgt cggcaccagt gcgaccgaca accgtatcga ctctgtctat gacgacgaag
    21361 gtcagctgcg cttcacgctt gatgccaatg cgcggccgac cgaatttgtc tatgacgcgt
    21421 cgggcaacct catccacagc atcgactatg ccggctcgat cgcgccggcg gcgagctatg
    21481 cgctggcctc ggtgcaggcc caggtggcga gcctcgccgg cgacccggcc aaccgcacga
    21541 cgcgtttcgt ctatgatgcg gccgggcggc tcgcctatgt gatcgatgcc gccggcaccg
    21601 tcaccgccac cagctatgat gtcgtcggcc atgtcgtgaa gcagacggtg tcggcgacca
    21661 gctacaccgc tgtcggcgac cccgacctcg cctccatgca ggcctgggcg agcgccagtg
    21721 ccggcgctgc cgaccatgtc tcgcgcgtcc tctatgacgg ggtcggccgg ccggtctatg
    21781 cggtcgacgc cgaaggctat gtcaccgaga gccaatatga cgcgctcggc gaggtggtga
    21841 agacgatccg ctatgcggat cgctatacgg tcgacgacac caccaccacc gcccagctcc
    21901 agacactaat cggcacgctg cccgcgagcg cggtggtgac cagctatgcc tatgacacgg
    21961 ctggccgcgt caccgacatg accgacgcta ccagcggggt cacccattat ggctatgacg
    22021 cgctcggcca gcttaccgac acgacgctcg cctatggctc cgcggacgcc gtcaccaccc
    22081 acaccgccta tgacagtgcc gggcgcgtgc tgtcggtcac gcaaggctat ggcaccgcag
    22141 tggcggcgac caccagctat acgtacgatg cgctcggcca tgtcctgacc gtcaccgatg
    22201 cgcgcagcaa cgtcaccacc aacggctatg acgcgctggg ccgcgtggtg agtgtcaccg
    22261 atccgctgag cggggtgacc agctatcagt atgacgcgtt cggcaaccgg gtgaagacca
    22321 ccgacgtgcg cagcaatacg agctatgcct attatgacgc gctcgaccgg ctggtgctgc
    22381 aggtcgatgc ggcgggctat gcgaccgcga ccagctattc ggtcggtgac gcggtcctgt
    22441 cggtcaccca ttatgccacc aaggtgtcgg gcagctatgg ggtcggcacg ccgcctgccc
    22501 tcacggccag cgccacgaag gatgaggtca ccagcttcat ccgcgacaag ctcgaccggg
    22561 tgacccgggt taccgacgct gagggctatt atgagcagta cacgctcgat gccttcggca
    22621 accgggggac ggtcaggaac aagctgggtg gtatcaccac caacgtcttc gaccggcgcg
    22681 gattgctggt ctccgagaca aagcccgaaa cctcgacgcg ttccgatggc agcatcgagg
    22741 caagcagcgt caccaccagc tatgcctatg acgcgcgcgg caaccgtatc cagatgatcg
    22801 aggcggcggg tctcgccgag cagcgcacca ccagctacgc ctacgacaag ctcgaccgga
    22861 tggtctcgaa gaccggcgat gccttcgcga tcctcaacag cgacaagacc tcgaccagcc
    22921 tcgccacccc ggtcgagacc ttccgctacg atgcacgcgg caatctcatc gagcatgacg
    22981 acgccgccgg cgcgcgcacg ctgagctatt atgacgcgct cgaccgcaag atcgcttcgg
    23041 tcaacgcgct cggcacgctg agcgcctgga cctatgacgg caacggcaat gtgctcacca
    23101 cgcgcgtcta tggcgatgcg gtggcactgc cggcgacccc gggcggcacg ccgccggcac
    23161 cggtcaacag cgccaattat cgcgagacgc gctatgccta tgacaaggac aacaggcaga
    23221 tcgagacgcg cgtcgtcagc atcgacgtcg gcgagcacag caacggcact tacgttaatg
    23281 gcacgcagga tatcgtcacc gcgaccgcct atgacgcggc cggcaacatc atcagccaga
    23341 ccgatccgcg cggccatgcg gtctacagct tctacgacaa ggctggccac aagatcgccg
    23401 aggtcgacca ggaaaattac ctgacgagct gggaccggga tggcgacggc aatgtcgtca
    23461 ccgagacgcg ctacgccacc cggctctccg tcgtcgtcgg cacggccagt gatccggccg
    23521 cgctcaaggt caatgccggg agcagcgccg acgaccgcat tacccagttc agctatgatc
    23581 ggaacggccg gcggctgacc gagacgcggc tcaacctggt cgcctcgagc gtgtcggcga
    23641 ccggcgcgct gaccacggtc accaccaatg cgacggtgag ctacaactat aatggtctcg
    23701 gccaggtcac ccgcaagacc gaggccaatg gcgacactac cgactacagc ttcgatttct
    23761 tcggtcgcat gaccgagatc gccaggagcg gctatgtcga cagcaacggc gcctcggtgc
    23821 ggccgaccac cgacatgcaa tatgacgggc tcgatgaact gacccgccag ctggtgcgcg
    23881 gtaccgatgc caccaccgag gccgacgacc ggattaccag ctacgcctat gacaaggccg
    23941 ggcacgtcat caccatgacc gacccaacca acttcgtgac cagctcatac tacgacatcg
    24001 ccggccatct ggtgaagcag cagtgggtgc gtctgaagtc ggacggcagc ggcgagaccg
    24061 atgccgactt ctacagctat gacctgctcg gccaccagac catgcagcaa ctcggcatct
    24121 tcaacggtgg caccagctgg acgctgagcg agaccaccga catgcgctac gatgcctatg
    24181 gcgagatggt ggcgcgcggg atcagcaccg gcggcaatcc ggcaaactac caggaattcg
    24241 ccgactatga cgcgatgggg cgcgtctggc ggaccaattt caacgacggc gtcaccaagg
    24301 cctatgtcta cgacgccaac ggtaatgcca cgctgttgct gcagtcgacc ggcagcgctg
    24361 acctgaggac gctgacactt gcacagatgg tggcggccgg cagcggcgtc accgagacgc
    24421 agagcgtgtt cgacaagcgc aaccagctca tcgagacgat ccagccgagc atcgccaacg
    24481 ccgcccagct gccaggcagc ataaacgcct tcgataccgt caatgccgga tccaacttca
    24541 ccggcggcgc cgtctccgtt gggtccaccc gcggcgccac cggaagctcc ggcagcgtct
    24601 cagggactcc aggggcgctg gcggcggatc gggggactgt aacctttaca ctccaatggg
    24661 ctgcgcccca tggttccggc tcttacacac ctaattttag ctacgttggt aatatcggct
    24721 atggaggtgg agcatttcat ctttggatat ctggcccgca aaattacaat aaagaggttg
    24781 ttcctggtca gagatcattg gatgggcttg tgaatgggac atatactcta actctctacc
    24841 aggatgggcc ggctggaact gggcaggtat tcattacgca agggtcgatt gttgtaagcg
    24901 caacaagcgg atctgcgagt attcccgttt cgcctcctgc caatctccaa tttcagggcc
    24961 agaatccgaa cacgagcaag gtgatgttgt tcgtgcgacc gcaggggtcg tcgggcccgt
    25021 accagcgggt ggcgaccacg cagttgctca attcggccgg tgctgcggtg gcgggctggt
    25081 tcacggccga ccccaacgcg gcgccattca acgggctgcc cgccaatagc ggctgggacg
    25141 tgaaatatta tgcgatggat gccaacggca acacactcaa cgcgcagacc gcgacgctgt
    25201 cggtcgacgg ttcgggtaat cccagcttta gtaacatcgt ggcccagcct atcggcggcg
    25261 ccggcaaatc gatcctcacc cgcgaattca acggcgactg gctgatctcg tccgaacagg
    25321 cgccgggcac ccagagcgtg ctcatgtact accgcgtcgc cggcagcaat tccgcctggt
    25381 cgaccacctg gatgtccaac aacggctatg gccagcataa tctgtgggcg accagcgtca
    25441 ccaactgggg cgggccttac gaatattggc ttgaggactt ctcggggacc aacggcaccg
    25501 gctcgctgat cggcaagaat tatggcacct tctcggtcgg cggccagccg tcggcgctga
    25561 ccagctatgc cgaccagccc gagacggtgc acatctacaa ccagccgccc gcgacctcga
    25621 tgctgctctg gtacaagcca tcgggcacca gcaactggat ccaggcggcc aacccgacct
    25681 ggaacggcgc caccggttcg tgggactggg acgccaacgg catcacgcct gataagctca
    25741 acgactatgc ctacgacttc cgctacgaga cctataatgg cagcgtactg gtcaaccagg
    25801 cgcatggcca ggtccagctc ggctatgatc cggcggtctt ctccaatgtc atcgaccagc
    25861 tgcccgcgac cgtcgtgttc tcaccgccgc aggccaatgc cacgaccctc atcctcaact
    25921 atcgcgccgc cggttcgacc ggtgcctaca gcacgattac gctgagtcgt gacgcctcgg
    25981 gcaagttcag cgtcaacgcc gacgcggcgc gccctgccag cggcagcatc acctatgaat
    26041 atttctacga tctgcgcgac agcgccggca atccggtcgc gccggtcggc ggcgaggatc
    26101 atgccaccgg ctatctcacg atcagcgccg atcgctcgac cgacaccagg accttgcaat
    26161 gggtgctggt cggttcgctc gatccggctg cgaccatcga tcgccagcag gcctacaacg
    26221 ccttcggcga catcgtctcg gagaccgacg ggctcaaccg tgtcaccacc atggcctaca
    26281 atgtcatcgg caagctgatc ctcaagcagg cgccgctggt ctcggtcacc gacgagcatg
    26341 gcgtccagtc gcaggtgcgt ccgaccgagc gttattatta cgacctcgcc ggccgtctgg
    26401 tcggggtcga cgacgccaat ggcaaccgca acacgctggc gctgctcgcc ggtagcggcg
    26461 aagaggcggg cgagaacgcg atcacgacca aggagttcca tgccgatggc ggcgtgagga
    26521 gctatggtat cgacgtcttc ggcgatgtcc gcaccgtcac ggacgaactc aactttgtca
    26581 ccaccaacag ctacgacaag atggaccggc tggtccaggt cgcccatccg acccgtgtcg
    26641 gcggcaattc caacggtgtc gccctcaccg acttctacgc ctatgatggc ctcggccatc
    26701 gcatcaagca ttggaactca cagctcggca gcactgtcgt cgagacaacc gattatggcg
    26761 gcaaggggcg tgtgatccag acgcgggact ttgccggcaa tgtgacgagt tacagctaca
    26821 gctggtcgac gacgctgacc aatagcggca ccggcacggt ggggggattg atcaaatccg
    26881 agaccgacgc cagcggccac acttctgtcg agcaggacga ctatttcagc cgggtggtga
    26941 ccaagaccga tcttggcggc cacagcttca actatggcta cgacaaggcg ggcaacctca
    27001 tccagcagac taatacggcg gggcagagca tcgcttatgc ctattatggc aatggctata
    27061 tacgctcgat caccgacaac agcctgcacg tccagtcgac cttcgaatat gacaaggaag
    27121 gcaaccgcac gctcgaaagc tatatcggcc tcggcacgaa cccgatctat tatcaggtcg
    27181 ccgcgatcag ctatgacgag ctcaaccgga tcaagacctt caccgacgcc aaggcgacga
    27241 tctcctacga atatgacgcc aacagcaacc gccggcgggt gctgagctat tatcatgacg
    27301 gcatcaacgg tgacgcccag acccaggatt actggtacaa atatgacggc atgaaccgct
    27361 tcgtgctgac catgggcacg ctcagtggcg gggtcggcgg caccatccag accggcacca
    27421 ccggcgtcca gatcggctat gatgccgcag gccagcgcaa gttcgcgatc aatggcagcg
    27481 acggcagccg cgaggattac agctacaccg ccgacggcta tctcgaggac accaagatca
    27541 acggcgtgtt gcgcgcgcgc cgcgtcaacg acgcggtggg gcgggtgacc acctacagcg
    27601 aatatctcac cgacggcgtc accacgaatt tcagccaggc ctcgacctat gacggcgaca
    27661 accgcgtcac cgaccagatc gtcacccagc gccagagcga cggctcctac acctatacca
    27721 acgtccacaa cgactatcgc gccgacgccg gcggcggcgt ctacaccggc gccgatcagg
    27781 gggtgatcac ccacagccgc cagcagcagc agggctcctc ggtggtccag gacaccgtct
    27841 acggctatca atggtgggac gaggccaagc aggccagcat ccaggtcaag ggcaccgatc
    27901 ccaacaaccc caacaactat cagtgggcgc cgggcgcctc gcagctcagc tacgacgtca
    27961 acggccacct caagagcgcg gtgatctcag gcggtacggc ggcggtggtc acctacacca
    28021 acgacgccta tggccaggtg ctcgaacgcg agcagaccag cggcagcacg atcgggccgc
    28081 gccagctcta ttattatttc gacggcaacc ggatcggcga cgtcggcaat gacgggccga
    28141 gccaggtcga ttatgccgca gcgctcgccc agcgcggcac cgtggtcgcg cccggcgcct
    28201 ttcgcggcgg caagcccgtc gccagcgccg acttcgacga gaattaccag ccgatcgggc
    28261 cgagctaccc cggcatggcg gcaaccgctt acacggtgcg ttcgggcgat acgctcgcca
    28321 gcatcgccca ggctgtctgg ggggattcga gcctctggta tctgatcgcc gatgccaatg
    28381 gcctgaccgc cagcgacacg ctggtcgccg gccagcagat cagtatcccc aacaaagtcg
    28441 ccaacatcca caaccgcacc ggcgtctacc gggtctacaa tcccggcgag gcgatcggcg
    28501 acctcctgcc gacactgccg gccgaacctg cgccccccgt ccacaaccat ggcggctgcg
    28561 gtgtcttcgg gcagatcctc ctcgtcgccg tcgccgtcgc tgtctccgcg gtaacgtatg
    28621 gcgcgctggg gggggaggcg ttgcagggag tggcggcaat cgaagcgggg gccgctgccg
    28681 gcgccgctgg cagcgtggcg agccaggcgc tgggcgtcgc caccggcatc caggatcatt
    28741 tcagcttcaa gggcgtggcg ctcgccgcca tcgccggcgg gatcggcagc gggctcggta
    28801 aggtcgggat cctcaagaat gcaaccgact tcgacaaggt gctccagaat gttgcgcgcg
    28861 gagcgatcag caacgggctc acccagggcg tggcgttggc caccggcctg cagaaccagt
    28921 tcgactggac cggggttgct gccgggggca tcgccagcgg cgtggcgggg gcgttccccg
    28981 gggtcggcgg tcatggggtt gaccattatc tcgccgaggg gctttctggc agcgccgctg
    29041 ctgtcgccgc atccgccgcc cgctcgttac tctccggctc cgacttcggc gacaacatcc
    29101 tgcgcgagtt gcccgatgca ataggcgcca ccatcgggaa tgcggctgcc cacactgcag
    29161 tggcggagtt cgcctcaaga tcggggccaa cggcctacaa cacctataac ggtccccagg
    29221 atgctcgtgt aatcagcgat attgtcgtcg ttggtcggct ccaaaactgg gcggaccgct
    29281 tactccgtgg gctcgacgcc gtcgcagact gggcggctga ccataatatt gttacagcct
    29341 tggctgatcc gattagccgt caattggaca acgccggcct tcattcctca gcgaatctcg
    29401 tccacgctgc tggtacgttc gtagcttcgg gagagcgggc gatctaccat gacgccgttg
    29461 gcatcggacg agcaattgtg cacccagtcc agaccgagct ggccatcgca ggcgcactgg
    29521 ataccgccat tcaaagcaat gcgtcggatg tcctcaacgg cgcccggcag agctacaata
    29581 acttcgcgtc tgctaacatc gacacccaag cggggatgct cggcggggcc gcaggccacg
    29641 tcgcgttcgc gacactttct ggccccgttg ctgaagcatc attgggtcga atcgcgtccc
    29701 tagtcggaga tgctcgaatt gcctccattc cacttgatac tcgcggcctc gccgcagaca
    29761 gtgcagcggt tcgcgtgggt ccatatggcg acctaaccgc cgaactgaag ggaacaggaa
    29821 ttcaggccaa tcacctgaat caggacgccg cctttagaga tttgatcccg cgcaatgacg
    29881 gcctggctgt cggcatgcgt ggtaatgcgt ttactgaggt tggttcggcg cactacgaat
    29941 ttcattccag ccttgaggag ttctggagtc cctatcggg
//
LOCUS       DATGEM010000288         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_95116, whole genome shotgun sequence.
ACCESSION   DATGEM010000288 DATGEM010000000
VERSION     DATGEM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95116"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VKQ27_09230"
     CDS             complement(<1..1155)
                     /locus_tag="VKQ27_09230"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HLY89155.1"
                     /translation="MNAPMPRPSGFLYSPMFPLGPDRTPWKKLPIDGVRTVTCDGQTV
                     LRIAPDALSELAFQAYHDVSHLLRPGHLAQLRAILDDPEASSNDRFVALDLLKNANIA
                     AGGVLPMCQDTGTALVFGKKGQRVWVDGDEEEALSWGVHRTYTETNLRYSQMAPLSMF
                     EEVNTGNNLPVQFDIMSAPGEHHADELHLMFVAKGGGSANKSFLFQETRAILSPERLL
                     KFLDGKMRSLGTSACPPYHLAIVIGGTSAELTLKTVKLASTRWLDTLPTTGSKAGHAI
                     RDLEMEQKVLELSRTLGIGAQFGGKYFCHDVRVIRLPRHGASLPVGVGVSCSADRQIM
                     AKITPEGVFLEQLETDPAKYLPDTTDQHLGDDAVAVDLNRPMSEIRCQLSA"
     gene            complement(1161..1670)
                     /locus_tag="VKQ27_09235"
     CDS             complement(1161..1670)
                     /locus_tag="VKQ27_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HLY89156.1"
                     /translation="MEDDTEQRPESLLPYEEWMAEAMREVVALAVSHAAANGLPGEHH
                     FYITFRTDYPGVVMPPRLRAQYPQEMTIVLQHQFWDLKFDPVANTISVGLSFGGVPAS
                     MVVPLGAVTAFVDPYVRYGLRFTPVPAGEPPPEEPEAEPPPPQLEQPSETPQVVSLDA
                     FRRRPPTKE"
     gene            1796..>2601
                     /locus_tag="VKQ27_09240"
     CDS             1796..>2601
                     /locus_tag="VKQ27_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89157.1"
                     /translation="MDATPPVEAQPWQRTLWTMVGVQFVMSIAFSILSPIMPLFLPQL
                     GVVSAEAVDVWSGVLASVTSFVAIFTAPVWGSLADRYGRKLMVLRSTLGISIFTLLMG
                     VAGNPWHMLAFRAGMGALAGFNSAATVLVSTQVPEQRLGYSLGWLGTGQLVGSLIGPI
                     IGGAVADVTGSYRMPFFFAGAVSLVAFAGTWWFVPERFTPPTGGRQRHSLLAGLRMLA
                     RSKALLALIVVMLMAQFATQAVQPVVTLFVQEMLGQRPDLATLGGLAFSVT"
BASE COUNT          396 a          794 c          907 g          504 t
ORIGIN      
        1 ggctgacagc tggcagcgga tttcgctcat cggccggttc aggtccacgg cgaccgcgtc
       61 atcgcccagg tgctggtcag ttgtatccgg caaatacttc gccggatcgg tctccaattg
      121 ctcgaggaac acgccctcgg gcgtgatctt tgccatgatt tggcgatcgg cgctgcacga
      181 gacgccgacg ccgacaggca gcgatgcgcc atgccgaggc aggcggatca cgcgtacgtc
      241 gtgacagaag tatttaccgc cgaactgcgc gccgatgccg agcgtgcgcg acagctccag
      301 gaccttctgt tccatctcca ggtcgcggat cgcgtggccc gccttgctgc cggtcgtcgg
      361 gagcgtgtca agccagcgcg tggatgccag cttcaccgtc ttcagtgtca gttcggcgct
      421 ggtgccaccg atcacgatgg ccagatggta gggcgggcag gcgctggttc cgagcgagcg
      481 catcttgccg tcgaggaatt tcagcagccg ctcgggcgac aggatggcgc gcgtctcctg
      541 gaacaggaac gacttgttgg cgcttccgcc gcccttggcg acgaacatca ggtgaagctc
      601 gtcggcgtgg tgttcgccgg gagccgacat gatgtcgaac tgtaccggca ggttgttgcc
      661 ggtgttgacc tcctcgaaca tcgacagcgg cgccatttgc gaatagcgca ggttggtttc
      721 tgtgtaggtg cgatgcacgc cccaggacag cgcttcttcc tcgtcgccat cgacccaaac
      781 gcgctggcct ttcttcccga acaccagcgc ggtgccggtg tcctggcaca tcggcagcac
      841 gccacctgcg gcgatgttgg cgttcttcag cagatcgagc gcgacgaagc gatcgttgct
      901 gctcgcttcg gggtcgtcga ggatcgcgcg caactgcgcc agatggcctg gccgaagcag
      961 atgcgacacg tcgtgatagg cctggaatgc gagttcgctc agcgcatcgg gggcgatgcg
     1021 cagcacggtc tggccgtcgc acgtcacggt gcgcacgcca tcgatcggca gcttcttcca
     1081 cggcgtgcga tccggcccga gcggaaacat cggcgaatag aggaagcccg agggccgcgg
     1141 cattggcgcg ttcatcgcgg ttattccttt gtcggcgggc ggcggcggaa cgcgtcgagg
     1201 ctcaccacct gcggggtctc gctcggctgt tccagttgtg gtggcggcgg ttcggcttcg
     1261 ggttcttccg gtggcggttc accagcgggc accggcgtga agcgtagccc atagcgcaca
     1321 tacggatcga cgaaggcggt gacggcgccg agagggacca ccatcgacgc cggcacaccg
     1381 ccgaacgaca ggccaacact gatcgtattg gcgaccggat cgaacttcag gtcccagaac
     1441 tggtgctgca gaacgatggt catttcctgt ggatattgcg cgcgcaggcg cggcggcatc
     1501 accacgccgg ggtaatcggt gcggaacgtg atgtagaagt gatgctcgcc tggcaggcca
     1561 ttggcggcgg cgtgcgaaac cgccagcgcc accacctcgc gcattgcctc ggccatccac
     1621 tcttcatacg gcagcaggct ttccggccgt tgctccgtgt catcttccat cggtgatcct
     1681 cacgctccgg agcacagata gcgcgcgggg cgccgggggc aaaggggcgc gccggcgctt
     1741 tacagttcgg ctcaaccctc gaatgcttgc gcgatggaat cgaggcgaac cccgaatgga
     1801 cgctacgccg ccggttgagg cgcagccctg gcagcgaacg ctgtggacga tggtcggggt
     1861 gcagttcgtc atgagcatcg cattcagcat cctcagcccg ataatgccgc tgttcctgcc
     1921 gcagcttggc gtggtttcgg cggaggcggt ggatgtttgg tctggcgtgc tggcctcggt
     1981 cacctcgttc gtggcgatct tcaccgcgcc ggtctggggc agcctggctg accgttacgg
     2041 gcgaaagctg atggtgctgc gatccacgct aggcatctcg atcttcacgc tcctgatggg
     2101 agttgccggc aatccctggc acatgctggc gtttcgcgcc ggcatgggcg ctttggcggg
     2161 attcaattcg gcggcgacgg tgctggtttc gacgcaggtg ccggagcagc ggctgggcta
     2221 ctcgctgggc tggctgggca ccggacagct tgtcgggtcg ctgatcggcc cgatcatcgg
     2281 cggcgccgtg gctgatgtca ccggcagcta tcggatgccg ttcttctttg ccggcgcggt
     2341 gtcgctggtg gcgtttgccg gaacgtggtg gttcgtgccc gaacgcttca cgccgccgac
     2401 cgggggacgg caacggcatt cgctgctggc agggctgcgg atgctggcgc ggtcgaaggc
     2461 actgctggcg ctgatcgtgg tgatgctgat ggcgcagttc gcgacccagg cggtgcagcc
     2521 tgtggtgacg ctgttcgtgc aggagatgct cgggcagcgt ccggacctgg cgacgctggg
     2581 cgggctggcg ttttcggtga c
//
LOCUS       DATGEM010000289         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1252647, whole genome shotgun sequence.
ACCESSION   DATGEM010000289 DATGEM010000000
VERSION     DATGEM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252647"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..553
                     /locus_tag="VKQ27_09245"
     CDS             278..553
                     /locus_tag="VKQ27_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89158.1"
                     /translation="MAGDRADDTRSRGRRPKAPVDAPAQREGVLGLLGDEAVTLAKVF
                     GSAIRDAPNQRVEGRKEAASRFADVPWYRPDVRLGRTAADTGAGRGQ"
     gene            543..1217
                     /locus_tag="VKQ27_09250"
     CDS             543..1217
                     /locus_tag="VKQ27_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89159.1"
                     /translation="MVNEVADAYRAYQQPFRHSGDRQTKTPAATNAAALGRGCAGATN
                     LIFPPLTGLIKGSGLANDIRSATGLSNDAAANAAMTAGGGIKLGAAGAVERAGARAVG
                     KLNPVRDATRKLMAPGTVDDNARAAATMHRMSIGTRRAEAESQFHALEDHQRVVGNAS
                     LQDQHDLARFIDTRGRAVPLRNSKLQPAADAIRDIAKRYRTWIENALSDDGGSSEFKE
                     DYYPRL"
     gene            complement(2275..2430)
                     /locus_tag="VKQ27_09255"
     CDS             complement(2275..2430)
                     /locus_tag="VKQ27_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89160.1"
                     /translation="MADEMSIASSSDGMRGQMDFSAAGAWCASLLICAGGVAVLLWLG
                     LSGTHTR"
     gene            2562..3335
                     /locus_tag="VKQ27_09260"
     CDS             2562..3335
                     /locus_tag="VKQ27_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HLY89161.1"
                     /translation="MRYEISGTVMQTVSIDLDRGERIYSQTHAMAWMSDGIDMATHTG
                     GGLFAGLRRAVSGGSLFITDYSAEQSGHIAFAPRFPGKILAMTLQPGQSLICRKETFL
                     CAESSVTFDIALQQRLGAGFFAGEGFILQRVTGPGTVFLDLSGEVVEKTLAPGERLRV
                     HAGHIGIQEPSVSTDIQMVRGFRNVLFGGEGLFLATLTGPGKIWLQSMPILNLAEEIA
                     RHLPGNESARGGRGVGAALGGGAAGAAIGGLLGGLLGNE"
     gene            complement(3343..>4098)
                     /locus_tag="VKQ27_09265"
     CDS             complement(3343..>4098)
                     /locus_tag="VKQ27_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89162.1"
                     /translation="SHVERTQPIFDLAGITHFAGLPTMPGVPEPEWAEAERKRCTMPF
                     YWDPFGDPARCGAISSRFVGEPGPSPILRDWIVAIATHPIAYAEHRLAHLNATLRIGG
                     RYDERGATAPGDSEPNPHGLGQPGGPLTHGIERLAAVVAETPLGSPAVWLVAVAAIGW
                     TLHGTARQPARDLGLALALSAMLMTASFAVVSIASDLRYHLWLMAATALAAVLLAGCT
                     GIDRRRLRWSLALIAVACVASVVARAYGVPVRF"
BASE COUNT          776 a         1377 c         1277 g          668 t
ORIGIN      
        1 cgggctaggc attttggcgt ggctgtagtt ccgcgccgcc ctcccgtctg catcctgggg
       61 ccgacacctc tccccccacc gatttcgatt tggacgcctc gcaaatacta tccgctgcat
      121 ttcgacactg aaatcgatgc aactacaagc actagcatat atctcatcgc gtatagtaat
      181 tagcactatg actaattgca gcattcttta ctaaattctg tatttgaacg atctaccaat
      241 ataagatcac ttcccagttt tatggtggag atcttcgatg gcaggcgacc gcgcggacga
      301 cacccgcagc cgtggccgcc gtcctaaggc cccggtggac gcgcctgcgc agcgagaggg
      361 tgtgctcggc ctgctcggcg acgaggccgt gaccctggcc aaagtgtttg gctcggccat
      421 ccgtgatgcg cccaaccagc gcgtagaggg gcggaaggag gccgcttccc gctttgctga
      481 cgtgccgtgg tatcggccag acgtccgcct tggccgcacg gcggccgaca caggggcggg
      541 gcgtggtcaa tgaagtggca gacgcatatc gcgcctacca gcaacccttc cggcacagcg
      601 gcgaccggca aacgaagacg cccgccgcga cgaacgctgc cgcgcttggc cgtggctgcg
      661 ctggcgcgac caacctgatt ttcccacccc tcaccggcct gatcaaaggc agcggcctcg
      721 ccaacgatat tcgatcggcc accggcctgt cgaacgatgc ggcggcgaac gctgcaatga
      781 ccgcaggcgg tgggatcaaa ctgggcgctg ctggcgctgt cgagcgcgcc ggcgctcgcg
      841 ctgttggcaa actcaacccc gtgcgagatg caacgcgcaa acttatggcg ccgggcacgg
      901 tggacgataa cgctcgtgcg gccgcgacca tgcatcgcat gtcgatcgga acgcggcgag
      961 cagaagccga gagccaattt cacgcgcttg aggaccatca gcgcgtcgtc ggcaatgcta
     1021 gccttcagga ccagcacgac cttgcccgct tcatcgacac gcgcgggcga gcagtgccgc
     1081 ttcgcaactc caagctccag ccggccgccg atgccatacg cgatatcgcc aaacgctacc
     1141 gcacgtggat cgaaaacgct ttgtctgacg acggcggcag cagcgagttc aaggaagact
     1201 attacccgcg gctgtgaaag aacccaccga gcgaagtcca ggccactatc gatcgtaacg
     1261 gctacggggg cggcaaacag ggtagcggca ccaatctcaa ggggcgccgc atcccgacct
     1321 atgccgaagg cttggccatg ggcttcaagc ctgtctacga gaacccgctc gacgcgatga
     1381 aggcctatgc cggctacatg gctgtgcgac aaccacgaca tccagttctc caagctactc
     1441 agtcgtcttg gtctgctttg gtcctgtagc ccgcccgaac tcgaaacctt ggcgcgcgag
     1501 atacgccacg cctgtagctg ggtcgtgcca ccctcccggc acgagcgtca cttcaatgtg
     1561 cgagagacca tcgggtacgt tcagagacgt ggcgcgtcac ccctatttca ttgcatgccg
     1621 tccgccgaca agcacggctg gcaaagctgg ctgatctgat ggccacagcc ccatctccgc
     1681 agtgacagct tatggtgcga tcccgctcga aaatgacagc tttgggtgct caaaatgacc
     1741 agcggctgat atgggtccgc acgaaaacgg gagattacga cagagtggct gacagcttat
     1801 gcgctaggtc acagcgcacg atgtttatgg aaagcctacg cgcatcgcgg ggcctcgctc
     1861 tcgaaaacac atgccttgat gcatagctgt acctcgccac aaagcgatga caagagaacg
     1921 atggcaaacg aagcaagctc gtcgaggaag ccactgccgg gcgagccccc tcgtcccggc
     1981 cgagaagaag cacagcagga tttacgcaat cgcgccttat ccctggcgag cttctgggag
     2041 gccgtcgacc caccacccgc gcatcccgag gggcagccaa aacctctcaa ggcatgctga
     2101 aacacattat gatggaatga cgatgatgat gaagcttttg gtatttgcgg cgctgtccgc
     2161 cgtctcgacc gcgtcggttg ccagtgcgga gcaacgcatg gccgtgaacg aggccggtca
     2221 ttgggaatgg cagtcgacgc cctcctatgg tcctcgagct cccttgccgg cccctcatcg
     2281 ggtgtgggtg cccgatagcc ctaaccatag cagcaccgcg acaccgcctg cacagatcag
     2341 cagcgaggca caccaggctc cggcggcact gaaatccatc tgaccgcgca tcccatcgga
     2401 ggagctggcg atggacatct cgtccgccac cgtcggttcc tcctggtgcg ttcgcgtctg
     2461 gtcacatgcg cgcgaagcgc cttgtgacgc acatcgaaaa gccgatgacc acgctcgctg
     2521 ccgcgctata acccggccat cgacagcgaa ggggcgggcc gatgcgctac gagatttccg
     2581 gaaccgtgat gcagaccgtt tcgatcgacc tcgatcgcgg cgagcgcatc tacagccaga
     2641 cgcacgcgat ggcgtggatg tccgacggca tcgacatggc gacccacacc ggcggcggcc
     2701 tcttcgcggg cctgcgccgc gcggtcagcg gcgggagcct gttcatcacc gactatagcg
     2761 ccgaacagag cggccatatc gccttcgcac cgcgtttccc cggcaagatt ctcgcgatga
     2821 cgctccagcc cggccagtcg ctgatctgcc gcaaggaaac cttcctctgc gccgaatcct
     2881 cggtgacgtt cgacatcgcg ctccagcagc ggctcggtgc cggcttcttc gcgggcgaag
     2941 gcttcatcct ccagcgcgtg accgggccgg gcacggtgtt cctcgatctc tcgggcgagg
     3001 tggtggagaa gacgctggcg ccgggcgagc ggctgcgcgt ccatgccggc catatcggca
     3061 ttcaggaacc gagcgtctcc actgacatcc agatggtgcg gggcttccgc aatgtcctgt
     3121 tcggcggcga gggtctgttc ctcgcgacgc tcaccgggcc ggggaagatc tggctgcaat
     3181 cgatgccgat cctcaacctc gccgaagaga tcgcgcgcca cctgcctggt aacgagagcg
     3241 cgcgcggcgg ccggggcgtc ggcgcggcgc tgggcggagg cgccgcgggc gcggcgatcg
     3301 gcgggctgct cgggggtctg ctcggcaacg agtgaggccg gatcagaagc gcaccggcac
     3361 tccgtaggcc cgcgccacca ccgacgcgac gcaggccact gcaatcagcg ccaggctcca
     3421 acgcagccgc cgtcggtcga tccccgtaca gcctgccagc agcaccgccg ccagcgccgt
     3481 cgccgccatc agccagagat gatagcgcag gtcggacgcg atgctcacca ccgcgaagct
     3541 cgcggtcatg agcatcgccg acagcgccag tgcaaggccg agatcgcgcg cgggctgtcg
     3601 cgcggtgccg tgcagtgtcc agccgatcgc cgccaccgcc accagccaca ccgccggcga
     3661 gcccagcggc gtttccgcca cgaccgccgc cagccgctcg atcccgtgag tgagcggccc
     3721 gccgggctgg ccgagaccat gggggttggg ctccgaatcc cccggcgccg tggcgccgcg
     3781 ctcgtcatag cggccgccga tccgcagcgt ggcgttcaga tgcgccagcc ggtgctcggc
     3841 ataagcgatc ggatgcgtcg cgatcgccac gatccagtcg cgcaggatcg gcgacgggcc
     3901 aggctcaccc acaaaacggc tgctgatcgc accacagcgc gccggatcgc cgaacggatc
     3961 ccagtagaag ggcatggtgc agcgcttgcg ctcggcctcg gcccattccg gctcgggcac
     4021 gccgggcatc gtcggcaatc cggcaaagtg cgtgatgccg gcgagatcga agatcggctg
     4081 ggtgcgctcg acgtgcga
//
LOCUS       DATGEM010000290        19273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_324878, whole genome shotgun sequence.
ACCESSION   DATGEM010000290 DATGEM010000000
VERSION     DATGEM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 19273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19273
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324878"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..964
                     /gene="paaA"
                     /locus_tag="VKQ27_09270"
     CDS             53..964
                     /gene="paaA"
                     /locus_tag="VKQ27_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HLY89163.1"
                     /translation="MDAEERIEPNDWMPDAYRRTLVRQISQHAHSEIVGMLPEGNWIT
                     RAPTLRRKAALLAKVQDEGGHGLYLYAAAETLGVSREELVDQLLTGRAKYSSIFNYPT
                     LTWADIGAIGWLVDGAAIMNQIPLCRCSYGPYARAMIRICKEESFHQRQGFEIMTTLA
                     HGSEEQRAMAQDALNRWWWPSLMMFGPPDSQSQHSDASMRWKIKRFTNDSLRQKFVDA
                     TVPQGHYLGLTFPDPDLGFNETTGHWEFGVIDWDEFRRVLTGDGPCNRERLAARHRAH
                     EDGAWVREAALAYAAKQRSAPRREAAE"
     gene            961..1248
                     /gene="paaB"
                     /locus_tag="VKQ27_09275"
     CDS             961..1248
                     /gene="paaB"
                     /locus_tag="VKQ27_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HLY89164.1"
                     /translation="MIETATPLWEVFIRARTGLAHRHVGSVHAADATLALQAARDVYT
                     RRGEGLSIWVVPSNAISASDPSDKDMLFEPTASKIYRHPTFYEVPDEVDNM"
     gene            1308..2567
                     /locus_tag="VKQ27_09280"
     CDS             1308..2567
                     /locus_tag="VKQ27_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY89165.1"
                     /translation="MNAPVNFDPREDAASLPIDRIDVSQPRLYQDDIWEPYFERLRRD
                     DPVHWSEGSMYGDYWSVTKYRDIMAVDTNQHVFSSDALMGGIVLRDAPMDYRRPSFIS
                     MDQPKHDEQRKVVSPVVAPSNLQRLSGTIRERTQRVLDELPRGEPFDFVDRVSIELTT
                     QMLATLFDFPFEERRKLTYWSNVATVNTRAGTEIDSDEKREAVLQQMMEAFTELWRER
                     AAQPPRPDLISMLAHGEATRDLPSRPAEFMGNTLLLLVGGNDTTRNSMSGGLWALHNH
                     PDQYRKLMTNPELLTGAISEIIRWVTPVIHMRRTALRDTELGGKRIRKGEKVAMWYIS
                     GNRDEDGIERANEFLIDRARPRQHLSFGFGIHRCVGNRLAELQLTSLWEEMLKRFPMI
                     EVLESPTRVYSNLLRSISSMKVRIPVH"
     gene            2677..4185
                     /locus_tag="VKQ27_09285"
     CDS             2677..4185
                     /locus_tag="VKQ27_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY89166.1"
                     /translation="MLLPASPRTRFIGATALVIAIAVMARAFELGSQSLWTDELFTRY
                     YPSLFSLKFLWTTGLFHENSPPLYYIAIKAWMALFGSSETALRSLSLLASVLTLPLVY
                     LLAMELFHQRRHGLLAMLAFSLLPMQIGFAQEARTYALLLIPLCGALLAIARMLRGDW
                     QWQTAVLYGFCATAALYCHVIAAFFLVACNIVVIAATARHQRQAWRRWVAINALVFVL
                     TIPELMAILGEGRSGSGISWIPPFRPVDVVRALSPVVVGSATPDKVPGAELALLLLAV
                     LAVRQILVPSARSTLLVLLAIPCIYVLLIATASLLHPIFIARMFCWLDLPLALLLADA
                     IATPWRYRSVAAGMACAAIGTGLGYQYTTIHNEPWRSIFHQIGPQLAHADEVVLAPLT
                     DPTTIAYYAPYLGHPRMLDTAPHGNVENGLMPDRMNVHWVTPAQVIDRVHSGAEVWLI
                     MRPPDAPYVGSLLTELPPPSTRIARSCDKVICIMALAWSRHATPASTGFDVR"
     gene            4457..6046
                     /locus_tag="VKQ27_09290"
     CDS             4457..6046
                     /locus_tag="VKQ27_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89167.1"
                     /translation="MKLWLGPQTLVLGSVGICIGILICVCARHIARLSTLVLLDRLAS
                     NDRAVGVIIIAATVLPAAIIFLAFDRFANSADEGAFLFQATTFLHGHLWDAAPAEPGL
                     FAQNDVVAHNGIWISQYLPGWPAVLALFQAIHLPWLAAPMCGAALLLLLWTALRLECT
                     RPAMVVPILAAYASTGFFLLNASTYFSHCVSAVTVVGSIVCILRAEGNPSPWWPVAAG
                     VCFGAALLCRVDSAALVGTAILAGWIEQGCKRRTFLLGFVGAAPLLALFCIYNTLVTG
                     SPLIVPTAWAGNITLGTHGLIGTDAPEGRFRMLVQTAWRTGELADTASLVLPGLYFVS
                     LYLRLRSRNFRFFDFVPLANFAVFLIYPDLGGFQMGPRYWFDGFIVMHITVASVFSRQ
                     TVEWQRFIVACCLLLVPVSLARLPGQVLFEARVMHERSSVFRLAAALPRDGRSIILIN
                     DFDSAFNDRANRTAPNLAMDFVRNGINLDKPVLFARGDVPDALPRACALYPQASIFAF
                     NLDSAHPDGWLAPLACAPHGA"
     gene            6080..6829
                     /gene="paaC"
                     /locus_tag="VKQ27_09295"
     CDS             6080..6829
                     /gene="paaC"
                     /locus_tag="VKQ27_09295"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HLY89168.1"
                     /translation="MATTSITVKETPLVRQLLYRADDALILGHRLSEWTGHAPMLEEE
                     LALANIALDLIGQARLLFAYAGELTNHTEDQLAYLRDTLAWHNCLLVELPNGDFAFTI
                     ARLMLFAAFADPFWRAATASSDNTVAAIAAKAEKETTYHLRHATEWLIRLGDGTNESH
                     RRAQSAIDDLWPYTGELFAADEGDVMPNTSLLRDIWSGTLDTALVRATLARPADGWMQ
                     SGGRAGRHTEHLGYLLAELQYLQRTYPGAPW"
     gene            6841..7311
                     /gene="paaD"
                     /locus_tag="VKQ27_09300"
     CDS             6841..7311
                     /gene="paaD"
                     /locus_tag="VKQ27_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HLY89169.1"
                     /translation="MRERALAALRDVCDPEIPVLTIGDLGILRHVSVDGDTVDVTITP
                     TYTGCPAMSMITLDIETALSHAGIKARVHTSLSPAWTTDWLTDAAREKLAAYGIAPPP
                     HTSSRRALFAAAQSACPRCGSTDTEQLSEFGSTACKSLHRCRACREPFDAFKCH"
     gene            7323..7715
                     /locus_tag="VKQ27_09305"
     CDS             7323..7715
                     /locus_tag="VKQ27_09305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY89170.1"
                     /translation="MPRQAIVTEQAPQPGGHFAQAIAVEATGRLVFISGMVAQRPDGT
                     IAGVGDIEAQTRQVCENVKAAVEAAGGTLANVCRVDVYVRNMENLAPIHKVRREYFPA
                     PPPASTLVEVSKFTHPDYLIEMSAIAVI"
     gene            7731..8612
                     /locus_tag="VKQ27_09310"
     CDS             7731..8612
                     /locus_tag="VKQ27_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89171.1"
                     /translation="MTSLRGNSGPRYKHATDESAPYDTITVDKLTPIIGAEIGGVDLS
                     QPLGNQTFEEIHRALAENSVIFFRDQHLSEDQHLDFGRRFGKLHLHPAAPHAPDRPEL
                     MVIHADKDSPRANGEGWHSDVSCDTEPPMGSILYIRTCPPHGGDTLFASMYAAYEALS
                     DRMKAYLDGLTAIHDGEAAYRGTYANYGISDKVTYPRAEHPIVRTHPVTRRKALYVNR
                     GFTQRIVGVPRDESSAILHYLWDHAENELFQCRFRWRENSIAFWDNRCVQHRAMWDYW
                     PHTRSGHRVTVQGDRPV"
     gene            8612..8998
                     /locus_tag="VKQ27_09315"
     CDS             8612..8998
                     /locus_tag="VKQ27_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY89172.1"
                     /translation="MANAATSPLGVYLEHLEKGELAYQFSPDANAAVFYPRIICPYTG
                     KDNLEWRISEGLGTVHATTAVYPQKGDPYNVALIDMDEGFRLMSRVEEFPPLDVCIGM
                     RVKLRVHPAEGDEPPYPVFIPAGDAP"
     gene            8995..10149
                     /locus_tag="VKQ27_09320"
     CDS             8995..10149
                     /locus_tag="VKQ27_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HLY89173.1"
                     /translation="MNGLARGSAAIVGAAESDIGAVAANMSPIDLMAQGIHRALADCG
                     LALKDVDGLWCATTQARTSAMSLCEYLRLPNAYTDSTIVGGSSFEVHVAHAQAALAAG
                     LCSVAVIAYGSTQRTVGRRAASAREHNPYETPFRPFLPATAYALAASRHMYDYGTTRE
                     QLAEVAVAAREWALLNPMAWEKKPLTVADVLNARPIAHPFTVRDICLITDGGGAIVMI
                     SADRARSLAKPPVYILGCGQAITHASISSMPNLTTTGARESGAAAYRMARMRESDIDV
                     LELYDAFTINTVLFLEDLGFCPKGEGGRFVSGGRIGPKGSLPVNTNGGGLSYCHPGMY
                     GLFLLIEAVRQLRGECAARQVAQAETALVHGNGGVLSAQATVILGTQSTL"
     gene            complement(10231..11046)
                     /locus_tag="VKQ27_09325"
     CDS             complement(10231..11046)
                     /locus_tag="VKQ27_09325"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HLY89174.1"
                     /translation="MDRVAQLIRDCSIELSPRDEFTGEALRGLVKPDAAVFVNYPSSV
                     SHHDIVAACVRLRRAGFDPVPHVAARRLASFTQASDFLLRAVNEAGVERILLIGGDDS
                     PIGPFRASLDLLATGVVERSGVRAVSFAGYPEGHPAIDSATLDAALQAKISLARQAGL
                     VVSVVTQFGFEADPIERWIEARAEGCPVHVGIAGPASVATLAKFAIRCGIGTSLRALA
                     RGHTAFARVLVEAAPDELIGQLAASGAPIAGLHLYTFGGVRRVTEWIQRRVSG"
     gene            complement(11046..12416)
                     /locus_tag="VKQ27_09330"
     CDS             complement(11046..12416)
                     /locus_tag="VKQ27_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89175.1"
                     /translation="MSEALLRDIAEFCRRSHMAESTFGRLAVNDGKLVSRLRLGGRVT
                     TDTVDRVHAFIERAPQRPTVNGVPVPIPAIRAGPSAGTSGNFRFYDNRQKYLLFVTTC
                     SEKWVVAQRVGMELANIHPRPPALRLFDAGMGDGTVLSRTMRAMHARFPTMPFHIVGK
                     EISLEDVRLVLEKMPDRLFEHPATVLVMTNMYYSEAPWLKPNSVNAATSLVWHELSLS
                     GNTAHDFEDQITELGPFLAESWRAKVSQTSGNPVYEKPVVLVIYREDHKFLLDQVRPR
                     RGFTEAEYDLVIASQPYRARASLEFKARRVVAPLARALAPGGRLIAIHSHGDDPGLEI
                     IRRIWPDENPFTTDRHALLRATKAELGHAGRDLNFGAYADARSLFRYDMHTLPSEVSE
                     TIGTSTLFAAWNAAVYVAQIEDQRLSEAISSGAYLDATQSVLREHGGLWFWDESYVIS
                     RKRDSG"
     gene            complement(12525..14849)
                     /locus_tag="VKQ27_09335"
     CDS             complement(12525..14849)
                     /locus_tag="VKQ27_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY89176.1"
                     /translation="MAKYGIGQPVTRFEDPRLLRGQGRFINDVNLPGQVHAVIVRSPH
                     ARARIAAVDTRAARALFGVLAVFTEADLAADGLGTMRVSLPRKRPDGSPLFYSPHPGL
                     ARGEVRYVGDPVAMVIAETLSVARDAAELVEIAYEPLPSVTDTARVLVPGAPAVWEAC
                     PDNLSHIYEAGNRAAADAAIAGAAHVVRRRLVISRVYAQFMEPRGAIGTFDPGEERYT
                     LYADVQYPHRVRDALARNIFKVPESKIRVIAGDVGGGFGTKGWQYPEHRLVLWAARRL
                     GRPVKWSSTRSEAIQADEHARDNITEAILALDAEGRFVALRADTICNVGAYISAERNL
                     LSTFSNVATLSGVYAIPAAYTHVSVAFSNSNPTAPYRGAGRPEAIYVIERLIDEAARE
                     LGFDRVALRRRNLVPESAMPYRNAFGVNYDCGAFERGMDAALSLADYDGFAARREESR
                     TRGRLRGIGIVNAIERAASPGLEFAEIRFNPGGSAMVLMGSKNQGQGHETMFKQIVHE
                     KLGLDPAEVQYIDGDTDRVAFGIGTMGSRSMVIGGSALTVAAGKIVAKARKIAAHMLE
                     ADEADIEFADGRFSVAGTDRALTIKQVAMAAFQPARLPPGLEPGLYETGTFTPKQDTF
                     PNGCHICEVEIDPDTGAVELLSYGVSDDVGTVINPLGLKGQIHGGIAQGLGQVLMEQV
                     AYDPESGQLLSGSFMDYAMPRADTTCDVAVASNAVPTALNPLGAKGAGEAGTVGALPA
                     VMNAILDALRGVGVRDLDMPATSSRVWAAMAAAA"
     gene            complement(14853..15941)
                     /locus_tag="VKQ27_09340"
     CDS             complement(14853..15941)
                     /locus_tag="VKQ27_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HLY89177.1"
                     /translation="MKAAVLHEANQPLTIEEVEHAPLKRREVLVRTAFAGLCHSDLHF
                     MEGLYPFPTPCVLGHEAAGVIEAVGEDVTYVKPGDHVITCLSVFCGECPQCMTGHPNL
                     CENTEVKLMPGAARRLTWKGAVLNQAMNLSAFAEKMLVHEHAVVKVRDDVPLDRCALV
                     GCGVMTGVGAVFHAAKVEPGATVAVIGCGGIGLSAVNGAAMAGAARIIAIDTVPAKLE
                     VAKQMGATDTINASNADPVEMVKEMTGGGVPYSFEAIGLKATAEQSFQMLRPGGTATI
                     IGMIPFGTKIELHGADFLRDRKIQGTSMGGNRFRVDMPRLLEMYVQGKLKLDHMISGH
                     IKLNEINQGFAQMKQGTLVRQLIDFSAG"
     gene            complement(15997..16392)
                     /locus_tag="VKQ27_09345"
     CDS             complement(15997..16392)
                     /locus_tag="VKQ27_09345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLY89178.1"
                     /translation="MDLAERMQQELPPLPRWMGLKVTVAEPKRVVAELTVREELCTSG
                     SIMHGGAVMAFADTVGAIGTIVNLREGQGTTTLESKTNFFAGSPVGAKLVAEAVPLHR
                     GRRTQVWETRITNEQGRLVAKVTQTQMVL"
     gene            16561..17682
                     /locus_tag="VKQ27_09350"
     CDS             16561..17682
                     /locus_tag="VKQ27_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLY89179.1"
                     /translation="MEDIITLTQIEAPSFQEATRAKAWREMAEAHGLKNLQIDAEGNV
                     TGLRPGMGNGPLICVAAHLDTVFPAGTDLTVRREGTKLLAPGVGDDTRSLAVLLAWLR
                     AMDAAQIQTRADILFVADVGEEGPGDLRGMRYLFQKGPYKDRISAFITVDSPDMEHIA
                     TGGVGSKRYRIIFNGPGGHSYGAFGLVNPMFAMADAITRLGQIKVPVTPKTTYSASVT
                     GGGTSINSIPNSVWTDFDLRSEEPVELDRLEASFLAIIAKSVAAENTVRSTRNGPIAT
                     DVQKTGDRPAGQTDPSQELVQLAHTAIVAHGFQPRFESSSTDANIPMSLGIPAIKIGS
                     GGTGGRGHSLDEWIDVAPEPSIRGMAAGLATLLGLAGVV"
     gene            complement(17665..18879)
                     /locus_tag="VKQ27_09355"
     CDS             complement(17665..18879)
                     /locus_tag="VKQ27_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HLY89180.1"
                     /translation="MPTDMLDPPPRHRAGYVEFPVLHVRGGTSTGLVISERWAPEPQE
                     LREELLRHLMGLPLEGTLPGNKQITGLGRIVPTGNKVFFARMEEAGRARRIVSTFAQL
                     AADKAAVDWSVNCGNMTAALPLWALETGNADAGNADAFEIDIFNTNTKVTAASRMRRN
                     SDGTFLTAEIPGVDGAFPAVDLFLANPVGAKTGQLLPTGRPVDEIAGYQVSCVDVAVP
                     MVIARAADFGKTGHDRLEELDTDAGLMATLRSIWVEAGLRMGLKVGGRLMSAEELAQS
                     ETIPKICIVGAPRGAGHISVRYFTPQMAHRSMAVSGGCCLAAAALIPGSIAHEVARGL
                     PAAGAAYDEVDVGIENPAGVLEATIEARAAGVGVQVRKAAYRRSTQILLRGSVPLYRA
                     SDALRGVLHDAS"
     gene            complement(18912..>19273)
                     /locus_tag="VKQ27_09360"
     CDS             complement(18912..>19273)
                     /locus_tag="VKQ27_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89181.1"
                     /translation="ARIMPLAPAETAPVPQVDAAALRQRIVALSEEILGERERTVFLA
                     RCLAERRNMRRYESLASELGVTRERVFELEGSARRKIAAALARDGLLQQATALEDPSK
                     GRRRRIAQPMDLATSTQ"
BASE COUNT         3540 a         6824 c         5384 g         3525 t
ORIGIN      
        1 ccccaccggt cccgtcgaca ccagcgaagc tgccacgcgc tttcagtcgc gtatcgacgc
       61 ggaagaacgc atcgaaccaa acgactggat gcccgacgcc tatcgccgta ccctggtccg
      121 ccagatcagc cagcatgcgc attcggaaat cgttggcatg ctgcccgagg gcaattggat
      181 cactcgcgcc cccacgctgc gccgcaaggc cgcactgctt gccaaggtgc aggacgaagg
      241 cggccacggt ctctacctct acgccgctgc cgaaaccctc ggggtctcgc gcgaggaact
      301 tgtcgaccag ctcctgacag gccgcgcgaa atactcgtcc atcttcaact atccgacgct
      361 gacctgggcc gatatcggcg cgatcggctg gctggtcgat ggtgcggcga tcatgaacca
      421 gatcccgtta tgccgctgct cctacggccc ctatgcacgc gcgatgatcc gcatctgcaa
      481 ggaggaatcg ttccatcagc gccagggctt cgagatcatg accaccctcg cccacggcag
      541 cgaagaacag cgcgcgatgg cgcaggatgc gctgaaccgc tggtggtggc cgagcctgat
      601 gatgttcggc ccaccggact cgcaatcgca gcattccgac gcctcgatgc gctggaagat
      661 caagcgcttc accaacgact cgctgcgcca gaaattcgtc gatgcgacgg tgccgcaggg
      721 ccactatctc ggccttacct tccccgaccc cgatctgggc ttcaacgaga ccacaggcca
      781 ctgggaattc ggagtgatcg attgggacga attccgccgc gtgctcaccg gtgatggccc
      841 gtgcaaccgt gaacgcctcg ccgcccgcca ccgcgcgcac gaggacggtg cctgggtgcg
      901 cgaagccgca ctcgcctacg ccgcaaagca gcggtccgcc ccacgacgcg aagccgccga
      961 atgatcgaaa cagccacacc gctctgggag gtcttcattc gggcccgcac cggcctcgcg
     1021 catcgccacg tcgggtcggt ccacgccgct gatgctacat tggcgttgca ggctgcgcgc
     1081 gacgtctata cgcgccgcgg cgagggcctt tccatctggg tcgtcccgtc gaacgccatc
     1141 agcgcatccg atccgtccga caaggacatg ctgttcgagc ccactgcttc aaaaatctat
     1201 cgccacccga cattctacga agtccccgac gaagtggaca acatgtagcc cacagtgcac
     1261 gctgccgctc aatcaagcat cacgccggcc agcagggaag aaacacgatg aacgctccgg
     1321 tcaatttcga cccgcgtgag gacgctgcat cgcttccgat cgacaggatc gatgtcagcc
     1381 aaccgcgcct gtaccaggac gacatctggg aaccatattt cgagcgcctg cgccgcgacg
     1441 atcccgtgca ttggagcgaa ggcagtatgt acggagacta ttggtcggtg acgaaatacc
     1501 gcgacatcat ggcggtcgac accaaccagc acgtgttttc gtccgatgcc ctgatgggcg
     1561 gcatcgtgct gcgcgacgca ccgatggact accgccgccc cagcttcatc tccatggatc
     1621 agccaaagca cgacgaacaa cgcaaggtcg tctcccctgt cgtcgcgcca tcgaatctac
     1681 aacgactgtc cggcaccatc cgcgaacgca cgcagcgtgt gctggacgaa ctaccccgcg
     1741 gcgaaccgtt cgactttgtc gatcgcgtat cgatagagct caccacgcag atgctcgcca
     1801 cactgttcga tttcccattc gaggagcgcc gcaagctcac gtactggtcc aacgtggcca
     1861 ccgtgaacac gcgcgccggc acggaaatcg actccgacga aaagcgcgaa gccgttctcc
     1921 agcagatgat ggaggccttt accgagctct ggcgcgagcg cgccgcgcag ccgccacggc
     1981 ccgacctgat ttcgatgctg gcccacggcg aggcaacgcg cgatctgcca tcaaggccgg
     2041 cggaattcat gggcaacacg ctgctgctgt tggtcggtgg caacgatacg acgcgcaatt
     2101 ccatgtcggg cgggttgtgg gcattgcaca atcatccgga ccaatatcgc aagctgatga
     2161 caaatcccga gctgctcact ggcgcgatat cggagatcat ccgctgggtc acgccggtga
     2221 tccatatgcg ccgcaccgcg ctgcgtgaca ccgagctggg cggtaagcgc atccgcaaag
     2281 gcgagaaggt cgcaatgtgg tacatctcgg gcaatcgcga cgaagatggc atcgagcgcg
     2341 ccaacgagtt cctgatcgac cgcgcgcgtc cacgccagca cctttcgttt gggttcggca
     2401 tccaccgttg cgtcggcaac cggctcgccg agttgcagct caccagtctc tgggaggaga
     2461 tgctcaagcg tttccctatg atcgaggtgt tggaatcacc cacgcgcgtt tattcgaacc
     2521 ttctgcgcag catctcatcc atgaaggtgc gcataccggt tcactgatac gacgtcacgt
     2581 tcgctcgagc ctgccatcac atatgcagga ggcggctggc caagtgcgcg ctttgcgcgg
     2641 caggtagtgg ccaccttctc caatcgagga acgtgtatgc tactgccagc aagcccgcgc
     2701 acacgtttta ttggcgccac ggcgctggta attgcgattg ccgtcatggc gcgcgccttc
     2761 gagctgggca gccagtcgct gtggacggat gaactcttca cccgctacta tccaagcctc
     2821 ttcagcctga aatttctgtg gacaaccggc ctgtttcacg agaacagccc accactctac
     2881 tacatcgcaa tcaaggcctg gatggcgctg ttcggtagca gcgaaaccgc gctgcggtcc
     2941 ctgtcactgc tggcttcggt cctgacactg ccgctcgtct atctgcttgc aatggaactg
     3001 ttccatcaac gacgccatgg cctgctcgca atgctggctt tctccttgct gccaatgcag
     3061 atcggctttg cccaggaagc gagaacctat gctctactgc tgataccact gtgcggcgca
     3121 ttgctcgcca tcgcgcgcat gctgcgcggg gattggcaat ggcaaactgc tgtgctttat
     3181 ggtttctgcg cgacagctgc actctattgt cacgtcatcg cggcattctt tctcgttgcc
     3241 tgcaacattg tcgtcatcgc cgcgactgcg cgccaccagc gtcaggcgtg gcgccgctgg
     3301 gttgccatca atgcccttgt cttcgttttg accattccgg aactgatggc gatccttgga
     3361 gagggccgat ccggatccgg catttcgtgg attcctccgt tccgcccagt ggacgtggtg
     3421 cgcgcactca gtccggtcgt cgtcggttcg gcaacccctg acaaggtgcc gggagccgag
     3481 ctcgcactgt tgttgcttgc cgtgctggcg gtgcgccaaa tcctggtgcc gtcggcgcgc
     3541 agcacattgc tcgtgttgct cgccatacct tgtatctacg tcctgctcat cgctacagcc
     3601 agcctgctcc acccgatctt catcgcgcgc atgttctgct ggctggatct gccgctcgcg
     3661 cttctactcg ccgacgcgat cgcaacgccg tggagatatc gctccgtggc cgccggcatg
     3721 gcctgcgccg ccatcggcac aggattgggc taccaataca caactatcca taacgaaccc
     3781 tggcgaagca ttttccacca gatcgggcca cagcttgcgc atgccgacga ggtggttctt
     3841 gcgccgctga ccgatcccac caccatcgcc tactacgcac cgtatctcgg gcatccgcgc
     3901 atgttggata ccgcgccgca cggcaacgtt gaaaacggtc tgatgccaga ccggatgaac
     3961 gtacactggg tcacgccggc acaggtaatc gatagggtcc actccggcgc cgaggtatgg
     4021 ctgatcatgc gtccaccaga cgcaccttac gttggatcgt tactgacgga acttccgcct
     4081 ccgtccacac gtatcgcacg atcgtgcgac aaggtcatct gtatcatggc actggcatgg
     4141 tcccgccacg caaccccagc cagcaccggc ttcgacgtca ggtaagcgct tgcgacctcg
     4201 tgtcgccatc tcaccaactg gcacgatcag ttccacagcc ggggtgacaa aaccgtgagg
     4261 caaagcgtca cgcatgggac gccggccctg atctgcgata tatggctggt gtatgagtga
     4321 gtcgaagcga gtattgcctc ttcggcacct gctgccctcc gatctcaaga tcccgtcagc
     4381 ccatccgttt gttacgcctg caatttctct gggtttgcta tcgcttgcat tgtccgttgt
     4441 cacggtaaca tcgcggatga agctatggct cggccctcag acgctggtgc tgggcagcgt
     4501 tggtatttgt atcggcattc tgatctgtgt atgcgcacgc cacatcgcac gtttgtccac
     4561 cctggtgctg ctcgatcgcc tagcaagtaa cgaccgtgcg gtaggcgtca tcatcattgc
     4621 cgccactgtc ctgcctgctg cgattatctt ccttgccttc gatcgattcg ccaactccgc
     4681 cgatgagggt gccttcctgt ttcaggcgac gacgtttctg catggtcatc tgtgggacgc
     4741 cgcacctgcc gaaccgggac tgttcgcgca gaatgatgtc gtggcgcata acggcatctg
     4801 gatcagccag tacctgcctg gctggccggc ggtcctcgca ttgttccagg cgatccatct
     4861 gccgtggctc gctgcgccaa tgtgtggcgc ggcattgcta ttgctgctct ggacagcatt
     4921 gcgtctggaa tgcacgcggc cagccatggt cgtgccaatc ctggcagctt acgccagcac
     4981 cgggtttttc ctgctgaacg cctccaccta tttctcgcat tgcgtcagtg cggtcacagt
     5041 ggttggcagt atcgtctgca tccttcgagc ggagggaaat ccgtcgccat ggtggccggt
     5101 cgccgcgggt gtctgtttcg gtgccgcgct gctctgccgg gtcgacagtg ccgcgctggt
     5161 tggcaccgcg atactagccg gctggatcga acaaggctgt aagcgacgca ccttcctgct
     5221 cggcttcgtc ggcgcggcac cgctgctggc tttattctgt atctacaaca cgctcgtcac
     5281 cggcagcccg ctcatcgtcc ccaccgcctg ggcaggcaat ataacgctcg gcacccacgg
     5341 cctgataggt accgatgcgc cagagggccg ctttcgcatg ttggtgcaga ccgcgtggcg
     5401 gaccggcgaa ttagcggaca ccgcgtcgct ggtcctgcca ggcctctatt ttgtctcgct
     5461 ctacctccga ttgcgatcac gcaatttccg ttttttcgac ttcgtgccgc tcgcgaactt
     5521 cgccgtcttc ctgatttacc cagatctcgg tggcttccag atgggaccac gctactggtt
     5581 cgacggcttc atcgtcatgc acatcactgt cgccagcgtc ttttcccgcc agaccgtgga
     5641 atggcaacgc ttcattgttg cctgttgcct gctgctggtc ccggtcagcc tggctcgcct
     5701 gccaggccag gtgctgttcg aggcccgcgt catgcatgag cgatcgtctg tattccggct
     5761 tgccgctgcc ctgccgaggg acgggcgctc catcatcctg atcaatgact tcgatagtgc
     5821 attcaatgac cgtgccaatc gcacagcacc gaacctcgca atggacttcg tgcgcaacgg
     5881 catcaatttg gataagcccg tgctgttcgc acgcggcgac gtccccgatg cgcttccccg
     5941 tgcctgcgcc ctgtatccgc aagcctcgat attcgcgttc aatctggaca gcgcccatcc
     6001 cgatggctgg ctcgcaccgc tcgcctgcgc accgcatggg gcgtgatacg cccggcctaa
     6061 ccatgctaag ctcacgccca tggccacgac atccatcacg gtcaaggaga caccccttgt
     6121 tcgccagctt ctgtaccggg cggacgatgc gctgattctc ggccaccgcc tgtccgaatg
     6181 gaccggccat gcaccgatgc tggaagagga gctggctctt gccaacatcg ccctcgatct
     6241 cattggccag gcccggctgc tcttcgcgta tgcgggcgaa ctcaccaacc ataccgagga
     6301 tcaactcgcc tatctgcgcg acacactggc atggcacaat tgtctgttgg tggaactgcc
     6361 gaacggtgac ttcgccttca ccattgcgcg gctgatgctg ttcgccgcct tcgccgatcc
     6421 attctggcgc gcagcaactg cgtcgtccga caatacagtc gcagcgatcg ctgcaaaggc
     6481 ggaaaaagaa acgacctacc atctgcgcca cgcgacggag tggcttatcc ggctcggcga
     6541 tggcaccaac gaaagccatc gccgtgccca gagtgcgatc gatgatctgt ggccttatac
     6601 cggtgaactg ttcgccgctg acgaaggcga cgtgatgcct aacacctcat tgctgcgcga
     6661 catatggagc ggcaccctag ataccgcgtt ggtacgcgcc acattggcgc gccccgcgga
     6721 tggctggatg cagtccggtg gccgcgccgg tcgccacacc gaacatctcg gctacctatt
     6781 ggccgaactt cagtatctgc aacgcacata tcctggtgct ccatggtagc cacagcaaac
     6841 ttgcgcgaac gcgcgctcgc agcattgcgg gatgtctgcg acccagaaat cccggttctc
     6901 accatcggtg atctgggcat cctacgccac gtatcagtgg acggcgacac ggtcgacgtg
     6961 acaatcacgc ccacctacac cggctgcccg gcaatgagca tgataacgct cgacatcgaa
     7021 acggccctgt ctcacgccgg catcaaagca cgcgtgcata ccagcctgtc gcctgcctgg
     7081 accaccgatt ggctcaccga tgccgcgcgc gagaaactcg ccgcctacgg catcgcgcca
     7141 ccgccgcaca cttcgtcgcg ccgggcgcta ttcgccgccg cgcagtccgc ctgcccgcgc
     7201 tgcggcagca ccgacaccga gcagctcagc gaattcggct ccaccgcctg caaatcgctg
     7261 caccgctgcc gcgcctgccg tgaaccgttc gacgcattca agtgccattg aaggagcacg
     7321 ccatgcctcg ccaagccatc gtcaccgagc aggcgccgca acccggaggt cacttcgccc
     7381 aggccattgc cgttgaagca actggccgcc tcgtgttcat ctccggcatg gtcgcacagc
     7441 ggcccgacgg taccatcgcc ggtgtcggcg acatcgaggc ccagacccgc caggtctgcg
     7501 agaacgtgaa ggccgcggtg gaggctgccg gcggcacgct ggccaatgtc tgccgcgtcg
     7561 atgtctacgt gcgcaatatg gaaaacctgg cgccgatcca taaggtgcgc cgcgagtatt
     7621 tcccggcacc gccaccggcc tcgacgcttg tcgaagtcag caagttcacc catcctgact
     7681 atctgatcga gatgagtgcg atcgccgtga tctgattggg agagtcatcc atgaccagcc
     7741 tgcgtggcaa cagcggcccg cgctacaagc acgccaccga tgaatcggcg ccctacgaca
     7801 ccatcaccgt cgataagctc accccgatta tcggcgccga gatcggcggc gtcgacctgt
     7861 cgcagccgct cggcaaccag accttcgagg aaatccaccg cgcgctcgcc gaaaactccg
     7921 tgatcttctt ccgcgaccag cacctcagcg aggaccagca tctcgatttc ggccgccgtt
     7981 tcggcaagtt gcatctccat cccgccgcgc cccatgcgcc tgaccgtccc gaactgatgg
     8041 tcatccacgc cgacaaggat tcaccccgcg ccaacggcga aggttggcac agcgacgttt
     8101 cctgcgacac agaaccgccg atgggcagca tattgtatat acgaacgtgc cccccgcacg
     8161 gcggcgacac cctgttcgcc tctatgtacg ccgcatacga agccctgtcc gatcgcatga
     8221 aagcttatct cgacggcctg acggcgatcc acgacggcga ggcagcctat cgcggcactt
     8281 acgccaacta cggcatatcc gataaagtaa cctatccacg tgccgagcac ccgatcgttc
     8341 gcacgcatcc ggtcactcgc aggaaagctc tctacgtcaa tcgtggcttt acccaacgca
     8401 tcgtcggcgt cccgcgcgat gagagtagcg cgatcctgca ttacctgtgg gatcacgcgg
     8461 agaacgagct gttccagtgc cgcttccgct ggcgcgagaa ttccatcgcc ttctgggaca
     8521 atcgctgcgt ccagcaccgt gcgatgtggg actactggcc gcacacccgc tccggccatc
     8581 gcgtcactgt ccagggcgat cggccggtgt aatggcaaac gcggccacat cccccctcgg
     8641 ggtctatctg gaacacctgg aaaagggcga gctcgcctac cagttcagcc ctgatgccaa
     8701 cgccgctgta ttctacccgc gcatcatctg tccctacacc ggtaaggaca atctggagtg
     8761 gcgcatcagc gagggcctgg gcaccgtcca cgccaccacg gccgtctatc cacagaaggg
     8821 cgatccctac aacgtcgcgc ttatcgacat ggatgaaggt ttccgcttga tgagccgcgt
     8881 cgaggagttc ccgccactcg acgtgtgcat cggcatgcgg gtgaaattgc gcgtccatcc
     8941 ggccgagggc gacgaaccgc cctatcccgt cttcataccc gccggagatg cgccatgaac
     9001 ggcctcgccc gcggtagcgc cgcgatagtt ggcgcagccg aatccgacat aggcgcagtc
     9061 gcagccaata tgagcccgat cgacctgatg gcgcagggca tccaccgtgc gctcgccgac
     9121 tgtggcctgg cattgaagga tgtggacggt ctctggtgcg ctacgacgca ggcgcgtacc
     9181 tcggcgatgt cactctgtga gtatctacgc ctgccaaacg cctataccga ttccaccatc
     9241 gttggtggtt cgtcgttcga ggtacatgtc gcgcacgcgc aggccgcgct tgcggccggc
     9301 ctgtgctcgg tcgcagtgat cgcctatggc agcacgcaac gtactgtggg ccgccgcgcc
     9361 gcttcggcgc gcgagcacaa cccatacgag acaccgttcc gcccgttcct acctgccact
     9421 gcctacgcgc ttgccgcgtc gcgccacatg tacgattacg gcaccacacg cgaacagctt
     9481 gccgaggtcg cagtcgccgc gcgcgaatgg gcacttctca acccgatggc ctgggagaaa
     9541 aagccgctca ccgttgcgga cgttctgaac gcccgaccga tcgctcatcc gttcacagtt
     9601 cgcgatatct gcctgataac cgacggcggc ggcgccatcg tgatgatatc agccgatcgc
     9661 gcgcgcagtc tggccaagcc gccagtctat atcctgggct gcggtcaagc gatcacgcac
     9721 gcatccatct cgtcgatgcc aaatctcaca accaccggtg cgcgcgagtc cggcgcggcc
     9781 gcctacagga tggcgcgcat gcgcgaatcc gacatcgacg tacttgaact gtacgatgcg
     9841 ttcacgatca ataccgtgct gttcttggaa gatctcggct tctgcccaaa gggcgaaggc
     9901 ggccgctttg tcagcggcgg acgcatcggc ccaaaaggca gcctgccagt caataccaac
     9961 ggcggcggtc tctcctattg ccaccccggc atgtacggcc tgttcctctt gatcgaagcg
    10021 gtccgacagc ttcgcggcga atgcgctgcc cgccaggttg cccaggcgga aaccgcgctg
    10081 gtgcacggca acggcggcgt cctatccgcg caggccacgg tgatcctggg tacccagtcg
    10141 accttgtagc tacatctgtc attgcgagga gcgcagcgac gaagcaatcc cggcaagttc
    10201 gttccgttct gatggcaatt acacgggacg ttacccgctc accctacgct gtatccattc
    10261 cgtcacgcgc cgcaccccgc cgaatgtata tagatgcagt ccggcgatcg gcgcgccact
    10321 cgccgccaac tgcccaatca gctcgtctgg cgccgcctcg accagcacgc gcgcaaacgc
    10381 cgtatgccca cgagccaagg ctcgtagcga cgtgccaatc ccgcagcgga tcgcgaattt
    10441 cgccaaggtc gcgacactgg ccggcccggc aatccccaca tgcaccggac aaccctcagc
    10501 ccgggcctcg atccaccgtt cgatcggatc tgcctcgaaa ccaaattgcg tcaccaccga
    10561 cactaccagc ccagcttgcc gtgccaggga aatcttcgcc tgcaaagccg catccagcgt
    10621 ggcggaatcg atcgcaggat gcccttccgg gtagccggcg aacgagacgg cacgtacgcc
    10681 gctgcgctct acgacaccag tcgccagcag atcgagactt gcccggaagg gtccaatcgg
    10741 gctgtcgtca ccaccgatca gcaggatccg ctcgacgccg gcctcgttca ctgcccgcag
    10801 caggaaatcg cttgcctggg taaagcttgc cagccgtcgt gccgcgacat gcggtaccgg
    10861 atcgaacccc gccctccgca gccgcacaca ggctgcgaca atgtcatgat gcgacacgct
    10921 cgatggatag ttcacgaaca ccgcagcgtc gggcttcact agtccgcgca gtgcctcccc
    10981 ggtaaattcg tcgcgtggcg acaattcgat cgagcaatcg cggatcagtt gtgcaacgcg
    11041 gtccatcagc cggaatcccg cttgcgcgag atcacgtacg attcatccca gaaccatagc
    11101 ccgccatgct cccgcaatac tgactgcgtc gcgtccagat aagcgccgct gctgatcgcc
    11161 tcggacagcc gttggtcctc gatctgcgcc acatagaccg ccgcattcca cgcggcaaac
    11221 aacgtcgatg tgccaatggt ctcgctgacc tcgctcggca gcgtgtgcat gtcgtagcgg
    11281 aacaacgacc gcgcatccgc ataggcacca aaattcagat cgcgcccggc atgccccagc
    11341 tccgccttgg tcgcacgcag cagtgcatgc ctgtcggtgg tgaacgggtt ctcgtcaggc
    11401 cagatacgcc ggatgatctc gagcccggga tcgtcgccat gcgagtggat cgcgatcagc
    11461 cgcccccccg gcgccagcgc tcgcgccaga ggtgccacca cgcgacgcgc cttgaactcg
    11521 agcgacgccc gcgcgcgata gggctgcgag gcaatcacca gatcatattc agcctcggtg
    11581 aaaccgcgcc ggggccgcac ctggtccagc aggaacttgt ggtcctcgcg atagatcacc
    11641 agcaccaccg gtttctcgta taccggattg ccggatgtct ggctcacctt ggcccgccag
    11701 ctctccgcga ggaacggccc aagttcggtg atctgatcct cgaaatcgtg cgcggtattc
    11761 ccgctcagcg acagctcatg ccacaccagg ctggtcgccg cgttcaccga gttcggcttc
    11821 agccatggcg cctcggaata gtacatgttt gtcatcacca ggacggtggc aggatgctcg
    11881 aacagcctgt ccggcatctt ctccagtacc agccgcacgt cctccaggct gatctccttg
    11941 ccgacgatgt ggaacggcat ggtgggaaac cgcgcgtgca tcgcgcgcat ggtgcgcgac
    12001 agtacggtgc cgtcgcccat gccggcatcg aacaggcgaa gcgccggtgg gcgcgggtgg
    12061 atgttggcca gctccatgcc cacccgttgt gcaacaaccc acttctcgct acatgtcgtg
    12121 acgaacagca gatatttctg ccgattgtcg tagaagcgga aattgcccga cgtgccggcg
    12181 gatggccctg cccgaatggc tgggatcgga accggcactc cgttcaccgt tggccgctgc
    12241 ggcgcccgct cgatgaaggc atgaacccgg tctaccgtat ccgtcgtgac ccggccgccc
    12301 agccgcagcc ggctcaccag cttgccgtcg ttcaccgcca gccggccaaa ggtcgactcc
    12361 gccatgtgag agcggcggca gaactcggcg atgtcgcgta gcagcgcttc gctcaacgga
    12421 cgatctcccg gatcgctatt gtccggaagt cagccgccgc cagcgctcgt cgtcaaccac
    12481 aatcctgccc accataagag tgggcaacaa agtgggcaga ctgactacgc cgcggccgcc
    12541 atcgccgccc atacccgcga actggttgct ggcatatcca ggtctcgcac tcccacacca
    12601 cgcagcgcgt ccaggatcgc gttcatcaca gccggcaagg cacccaccgt gccagcctcg
    12661 cccgcgcctt tcgcccccag cgggttcaat gccgtgggca cggcattgct cgcaacagcc
    12721 acatcgcagg tcgtatcagc ccgcggcatc gcgtaatcca tgaagctgcc gctcaaaagc
    12781 tgcccggact caggatcata cgccacctgc tccatcagca cctggcccag tccctgcgcg
    12841 atgccgccat ggatctgccc cttcagccca agcggattga tcaccgtccc cacgtcatcc
    12901 gagaccccat agctcagcaa ctccaccgcg ccggtgtcgg gatcgatctc cacttcgcag
    12961 atatggcaac cgttcgggaa cgtatcctgc tttggcgtga acgtgcctgt ttcgtacagc
    13021 ccaggttcca gcccaggcgg cagccgtgcc ggctggaacg ccgccatcgc cacctgcttg
    13081 atggtcaacg cgcgatccgt tcccgcaacg ctgaacctcc catccgcaaa ctcgatatcg
    13141 gcctcatccg cctccagcat atgcgcggcg atcttccgcg ccttggcaac gatcttgccc
    13201 gccgccacgg tcagcgcgct gccgccgatc accatcgatc gcgaccccat cgtgccgatg
    13261 ccgaatgcca cgcgatccgt atcgccatcg atgtactgca cctcggccgg gtccagccca
    13321 agcttctcat gcacgatctg cttgaacatc gtctcgtggc cctggccttg gttcttgctg
    13381 cccatcagta ccatcgccga gccacccgga ttgaaccgga tttcggcaaa ttccaacccg
    13441 ggcgatgccg cccgctcgat cgcattgaca atgccaatgc cgcgtagccg cccgcgtgtc
    13501 cgcgactctt cgcgccgcgc cgcgaaaccg tcatagtctg ccaaggacag cgccgcatcc
    13561 atgccgcgct caaatgcacc gcagtcatag ttcacgccga acgcgttgcg gtacggcatt
    13621 gcgctctccg gcaccaggtt ccgccgccgc agcgccacac gatcgaaccc caactcccgc
    13681 gccgcctcat cgatcagccg ctcgatcacg tagatcgcct cgggccggcc cgccccgcga
    13741 tacggtgccg ttggattgct gttggagaaa gcgacagaca catgtgtata cgcagccggt
    13801 atcgcgtata ccccgctcag cgtcgccaca ttgctgaacg tcgacagcag attgcgttcc
    13861 gccgagatat acgcgcccac attgcagatc gtgtcggccc gcagcgccac gaacctcccc
    13921 tccgcatcca gcgccaggat cgcctcggtg atgttgtcgc gcgcatgttc gtcggcctgt
    13981 atcgcttcgc tgcgcgtgct cgaccacttc accggccgac ccagccgccg cgccgcccat
    14041 agcaccaggc gatgctcggg atactgccac cccttggtgc cgaacccgcc gccgacatcg
    14101 cctgcgatca cccggatctt gctctccggc accttgaata tgttgcgcgc cagcgcgtca
    14161 cgcacccggt gcgggtactg cacatcggcg tacagcgtgt agcgttcctc gccgggatcg
    14221 aacgtgccga ttgcgccacg cggttccatg aactgcgcat agacgcggct gatcaccaac
    14281 cgccgccgga cgacatgcgc cgccccggca atcgcggcat ccgccgctgc gcggttccct
    14341 gcctcataga tatgggaaag attgtcggga caggcctccc atactgccgg cgcgccaggc
    14401 accagcaccc gcgcggtgtc cgtcaccgaa ggcagcggct catacgcaat ctcgaccaat
    14461 tccgccgcat cgcgggccac gctcagcgtc tctgcgatca ccatcgccac cggatcgccg
    14521 acatagcgca cctcaccgcg cgccagcccc ggatgcggcg aatagaacag cggcgaccca
    14581 tccgggcgct tgcgtggcag gctcacgcgc atcgtgccga gcccgtccgc cgcaagatcc
    14641 gcctcggtga acaccgccag cacgccgaac agcgcgcgcg ccgcccgcgt atccacagct
    14701 gcgatccggg cacgcgcatg cgggctgcgc acgatcaccg catgaacctg cccgggcagg
    14761 ttcacatcat tgatgaaacg cccctgcccg cgcagcagcc gagggtcctc gaaccgggtg
    14821 accggttggc cgataccata cttggccacg tctcagccgg cggagaaatc gatcagctgg
    14881 cgcaccagcg taccctgctt catctgtgcg aacccctggt tgatctcgtt cagcttgata
    14941 tggcccgaga tcatatggtc cagcttcaac ttgccctgca catacatctc cagcagccga
    15001 ggcatatcca cgcggaaccg gttgccgccc atcgaggtgc cctggatctt ccggtcacgc
    15061 aagaaatccg cgccgtgcag ttcgatcttc gtgccaaacg gaatcatccc gatgatcgtc
    15121 gcggtcccac ctggacgcag catctggaac gactgctcgg cggttgcctt caggccgatg
    15181 gcttcgaagg agtacggcac gccgccgccg gtcatctcct tgaccatctc caccggatcg
    15241 gcgttcgaag cgttgatcgt atcggtggcg cccatctgct tcgccacttc aagtttcgcc
    15301 ggcaccgtgt cgatcgcgat gatgcgcgcc gcgccggcca tcgccgcgcc gttcaccgca
    15361 gagagcccga tgccgccgca gccgatcacc gcgaccgtcg cgccgggctc aaccttcgcc
    15421 gcatggaaga ccgctccgac acccgtcatc acgccacagc caaccagtgc gcagcgatcg
    15481 agcggcacgt catcgcgcac cttcaccaca gcatgctcat gcaccagcat cttctcggcg
    15541 aaggcggaca ggttcatcgc ctggttcagc accgcaccct tccaggtgag ccggcgcgcc
    15601 gcgccgggca tcagcttcac ctcggtgttc tcgcataggt tcggatgccc ggtcatacac
    15661 tgcgggcact cgccacagaa cactgacagg caggtgatga catgatcgcc aggcttcacg
    15721 tacgtcacat cctcgcccac cgcctcgatc acaccggccg cttcgtgacc cagcacgcaa
    15781 ggcgtcggga acggatacag cccctccatg aaatgcagat cggaatggca caggccggcg
    15841 aaagccgtcc gcaccagcac ctcgcggcgc ttcagcggtg cgtgttccac ctcctcgatc
    15901 gtcaggggct ggttcgcttc atgcagcacg gctgccttca tggttccctc cgtcacattc
    15961 ttgcccgagc ttagcctatc gccaggaaga gcgccgctac aacaccatct gcgtctgggt
    16021 gaccttcgcc accagccgcc cttgttcgtt ggtgatccgc gtctcccaaa cctgagtgcg
    16081 gcgtccgcga tgcagcggca ccgcctcggc gaccagcttc gcccccaccg gcgagccggc
    16141 gaagaagttg gtcttgcttt ccagcgtcgt cgtcccctgc ccttcccgca ggttcacgat
    16201 ggtgccgatt gctcccaccg tatcagcaaa tgccatcacc gcgccgccat gcatgatgct
    16261 gcccgacgta cacagttcct cgcgcaccgt cagttccgcc accacacgct tcggttccgc
    16321 caccgtcacc ttcagtccca tccagcgcgg caacggcggc agttcctgct gcatgcgttc
    16381 tgccagatcc atgcaaacct cccatcttgc cggcacgatc cgcctcagcc attaccgggc
    16441 ggacgcagcc ggagacgcca tgccatgacc agtcataccg agtcgattat ccgccaagtc
    16501 atggcgagcg aggcttaccg caccgccgtc gccactctcg ccgccgagca tgagcggaca
    16561 gtggaagaca tcatcaccct cacccagatc gaggccccgt ccttccagga agccacccgc
    16621 gccaaggcgt ggcgcgagat ggccgaagcg cacggcctga agaacctgca gatcgacgcc
    16681 gagggcaatg tcaccggcct gcgccccgga atgggcaatg gcccactcat ctgcgtcgct
    16741 gcccatctcg acacagtgtt ccctgccggc accgacctca ccgtccgccg cgagggcacc
    16801 aaacttcttg cccctggcgt cggcgatgac acccgctcgc ttgccgtcct gctcgcctgg
    16861 ctgcgcgcca tggatgccgc ccaaatccag acccgcgccg acatcctgtt cgtcgccgat
    16921 gtgggcgagg aaggccccgg cgatctgcga ggcatgcgct acctgttcca gaagggcccc
    16981 tacaaggatc gcatcagcgc cttcatcacc gtggacagcc ccgacatgga acacatcgcc
    17041 actggcggcg tgggctccaa gcgctaccgc atcatcttca acggtcccgg aggccatagc
    17101 tacggtgcat ttggcctggt caatccgatg ttcgccatgg ccgatgccat cacgcgcctc
    17161 ggtcagatca aggtgccggt tacccccaag accacataca gcgcgagcgt cacgggcggc
    17221 ggcacgtcta ttaactcgat ccccaatagc gtctggaccg acttcgacct gcgttcggaa
    17281 gaacccgtcg aactggaccg cctggaagcc agcttcctcg cgatcatcgc aaagtcggtc
    17341 gcagccgaga acaccgtccg ttccactcgc aacggcccaa tcgccaccga cgtccaaaaa
    17401 accggcgacc gccccgcggg ccagaccgac ccgtcgcagg aactggttca gctcgcccac
    17461 acagccatcg tcgcgcatgg ttttcagccc cgtttcgaaa gttcctccac tgacgccaac
    17521 atcccgatga gccttggcat ccccgccatc aagatcggct cgggcggcac tggcggccgc
    17581 ggccattcgc tcgatgaatg gatcgacgtc gcgcccgagc cctccatccg tggcatggca
    17641 gcgggcctcg cgacactgct tggactagct ggcgtcgtgt agcacgcctc gcagcgcgtc
    17701 agacgcgcga tatagcggca cactcccccg cagcaggatc tgcgtactcc gccgatacgc
    17761 ggccttgcgc acctggactc cgaccccggc cgcccgcgcc tcgatcgtcg cctccaggac
    17821 accagccggg ttctcgatcc caacgtccac ctcgtcgtac gccgcaccgg ccgccggtag
    17881 tccacgcgcc acctcatgcg cgatcgaacc agggatcagc gccgccgccg ccaggcagca
    17941 cccgcccgat accgccatag accggtgcgc catctgcggc gtgaaatacc gaaccgagat
    18001 atgccccgcg ccccgcggag ccccaacgat gcatatcttt gggatcgtct cactctgcgc
    18061 cagctcctcg gcggacatca gccgcccgcc aaccttcagc cccatgcgca gcccagcctc
    18121 cacccagatc gaccgcagcg ttgccatcag cccggcatcg gtatcgagct cctccagccg
    18181 gtcatgcccc gtcttcccga aatcggcggc ccgcgcgatc accatcggca ccgccacgtc
    18241 aacgcacgac acttgatagc cagcaatttc gtcgaccggg cgtcccgtcg gcagcaactg
    18301 ccctgtcttt gctcccaccg gattggccag aaacagatcc acggcgggaa acgccccatc
    18361 cacgccaggg atttctgccg taaggaacgt gccgtcgctg ttccgccgca tccgcgaggc
    18421 ggccgtcacc ttggtattcg tattgaagat atcgatctcg aacgcgtcgg cgttgcccgc
    18481 gtcggcattg cccgtctcca gcgcccacaa cggcagcgcc gcagtcatat tgccgcagtt
    18541 cacgctccaa tcgactgcag ccttgtccgc cgcaagctgc gcaaatgtgg agacgatacg
    18601 ccgtgcccgc ccggcctcct ccatgcgcgc aaaaaacacc ttgttgccgg tgggcacgat
    18661 gcgccccagc ccggtgatct gcttgttgcc aggtagcgtc ccctccagtg ggagccccat
    18721 cagatgccgc agcaactcct cgcgcagctc ctgcggctcc ggcgcccagc gctccgagat
    18781 caccaaacct gtcgatgttc ctccccgcac gtgcaacacg ggaaactcga catacccagc
    18841 ccgatggcgt ggcggcggat cgagcatatc agtaggcatc gagcggacct cggttgttcc
    18901 gaaatcgtca gctactgcgt cgaggtggcc aagtccatcg gctgggcaat acgccgcctc
    18961 cgccctttcg acggatcttc cagcgcggta gcctgctgca gcaacccgtc acgcgccaga
    19021 gccgcggcga tcttccgccg cgcgctgccc tcgagctcga acacgcgttc gcgcgtcaca
    19081 ccgagttcgc tggccaggct ttcatagcgc cgcatgtttc gccgttcggc gaggcagcgc
    19141 gcgaggaaca cggtccgctc tcgctcccct agaatctcct cgctcagcgc gacaatccgc
    19201 tgtcgcagcg cagccgcgtc gacctgtggg accggtgccg tctccgctgg cgccaatggc
    19261 atgattcgag cga
//
LOCUS       DATGEM010000291        13063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_912771, whole genome shotgun sequence.
ACCESSION   DATGEM010000291 DATGEM010000000
VERSION     DATGEM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13063
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912771"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VKQ27_09365"
     CDS             complement(<1..457)
                     /locus_tag="VKQ27_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89182.1"
                     /translation="MLNELPLRLHHHAYAVKDQETNRQFFEDILGIPLVATWCERTFR
                     PEVGREVDYCHTFYEVGDGSALAFFQFADEEAWEKNRAILQDVGGSWHVAFKVSQATF
                     DELMQRTETANVPVRKIDHGYCVSMYMKTPDGLRLEFTVDAPDADKIAAM"
     gene            525..1718
                     /locus_tag="VKQ27_09370"
     CDS             525..1718
                     /locus_tag="VKQ27_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLY89183.1"
                     /translation="MGKPRILIAGAGIGGIVAALALLQRGFEVALYEQAADLREIGAG
                     VQISPNGSRVLCHLGLQAAMEAIASVPTAKDMRLFSTGQAWRVQDLGATAVARFGSPY
                     WLVHRGDFHAVLVRALEERAPGAAHVGARCVLFEQDASGVTLLLGNGKRVRGDALIGA
                     DGVHSRIRGQLFGDGRATFTGYMAWRGVVPMERLPPHLRQPHGNTWIGPFGHVVTYPL
                     RRGELLNFVTAIERDDWLVESWSEAGTVDECRRDFARWHPDVLAIVDAIETPYKWAML
                     GRQPLEHWSVGRVSLLGDACHPTLPFLAQGANMAIEDGMVLARCLEAFADVPDALRHY
                     EAARLERTSRIVLGSLENVSRYHNPQLADPAEAQAFMDREFAPRAMGARYDWLYEYDA
                     LKVAV"
     gene            1813..2919
                     /locus_tag="VKQ27_09375"
     CDS             1813..2919
                     /locus_tag="VKQ27_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89184.1"
                     /translation="MRLSLVLAGFLLVGHLARADEPEFNIGVVASVTGPFAAPTKDTF
                     DGFNAWMKVHGLPGKKIVLQTVDDETNPVNASNAFRKLASDPKVSLIYLFINSNSAMA
                     AKSFASEFKVPIITGGGADALGVPADPWMFKVAPSNRDYMIALSEYIKRKGYTKVAHL
                     YSTDTFGQYDHTNLEKLAPQYGYRLVANESFAIDDTSFNAQFTRIRAAQPDLIYSSAS
                     ARAAILSFKQYKQLGMTTPLVVTGAAVSKAFFDAIGGPAEADGMMMMTQRGSLSAGLG
                     GESAKYYAELKDVLGRTPVFFNVFGFDVGLITAGAVEHSDGSRQGIRNALEKLKDLPA
                     INGPVTYTPVDHTGQDSRSITLGKLENGKSVPAE"
     gene            2919..3806
                     /locus_tag="VKQ27_09380"
     CDS             2919..3806
                     /locus_tag="VKQ27_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89185.1"
                     /translation="MDTTVFLQLLLPGLTTGCVYALVALGFVLCANVSGVVNFAQGEF
                     VMLGGMMAAWLIGLGVPFALAIIAATALGAAAGAAQEVLTLAPVRRQPHFIQITTTVG
                     FAVVLRGITLIVAGKDALSVPGFSGDGVFFLFGAILPVQSLWVWGATVLLLVGTFGFL
                     RYTSTGRAVRACSINLQAARLMGVNAERLRVIVFAVSGATGALAGVVITPIVLASWDA
                     GLSYSTKGFIGAIIGGFRSPSAAVAAGVFIGLLEALSAGYVSSGWKDFIVYGVLLAWL
                     LVQGGVFLRGRVDATAGGP"
     gene            3803..5533
                     /locus_tag="VKQ27_09385"
     CDS             3803..5533
                     /locus_tag="VKQ27_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLY89186.1"
                     /translation="MKYKAAIATASFLLAVVLYPLCYPDSYHLGVGISAGAMAVSTVG
                     VVLLLGLAHQLAIGQAAFSMIGGYANAILCVRYGWDPFVAMVAGAVLAMVLAYAIATP
                     LLKLRGFVLAMATLALHLMLIVVAVQVDFTGGPMGVTGIPKFAVFGLPLSNDRSFFWF
                     IWLLVFGAVWIGLRIDHSSIGRALRAIAASDQAAGSVGIDITRHKVQMFVIGGGMASV
                     SGSLLVHYLRATDPTVFSFGYSINLITGTIIGGLTSIWGGALGAAIIIFLRELLRELE
                     LPLWESVIMGALTVLVLIVFPRGIAGFVSRIYDRVVGDEAPRRVVVTEPGAVALPAMS
                     DPPALVLDGVSRSFGSLRAVNAVDFTVPAGAITALIGPNGAGKTTLFNLISGHLRLDA
                     GVVRFDGREIQALEPRDVARLGIGRTFQNLQLFDNMTVLQNVMCGHHRFGGAGAEQAA
                     RDTLAFVGLQGAERHEPASLPFGHQRLVEIARALALRPRMLLMDEPASGLNDAETERL
                     SELIVRVAALGVTVLLVEHDMRLVMGLADHVVVMDHGEKIAEGAPEPVRADPAVIAAY
                     LGQELPARAA"
     gene            5523..6281
                     /locus_tag="VKQ27_09390"
     CDS             5523..6281
                     /locus_tag="VKQ27_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89187.1"
                     /translation="MLLELDRVSSGYGRSPVLHDVSLTVGAGEIVALIGANGAGKSTL
                     LNTVMGLVATSSGEVRFDRRAITRMPTPAIVRAGIAQVPERRQLFGTMSVEENLLMGA
                     YACGDSSALEEQLARFPIMRERRHQLARTLSGGEQQMVALARAAMSRPRLLLLDEPSL
                     GLAPMIVARIMREIAALREAGGTVLIVEQNANAALGIADRGYVLENGRIALEGQAGEL
                     LVNPAVQEAYLGGHGGHYAIEARIRAKKIELLGR"
     gene            complement(6310..6840)
                     /locus_tag="VKQ27_09395"
     CDS             complement(6310..6840)
                     /locus_tag="VKQ27_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89188.1"
                     /translation="MGRRNYLIEGVSGAGKTAVCTELQRRGYQAIHGDRELAYRGDPQ
                     TGLPTAPEMAAPTATWMSEHQIWDVGKVQALVANHKEAVTFFCGGSRNFAKFIDLFDG
                     AFVLDVDVDTMNRRIDERVALDPTDFGGKPGERELAVRLHQTKQDIPKDAIIIDATAP
                     LARVVDEILRLSDASK"
     gene            7180..8292
                     /locus_tag="VKQ27_09400"
     CDS             7180..8292
                     /locus_tag="VKQ27_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89189.1"
                     /translation="MLVRLAAALFAALSVMAVPAAAQSEFKIGVVASLTGGFAAASTD
                     ENAGVQAWVKARGLSGRKIVFETLDDETNPVSAINAFRKVASDPSVSLIYALTNSNSA
                     MAIKSIASEFKVPIISSGAVETLGVPADPWLFKIAPGPRDMMIVLCEYAKKKGYTRLA
                     SLHSKDAFGQAEETYIKELAPKYGLKLVDVEEFNTDDTNFTPQIVKVKAADPQLIYNG
                     ATGRPGILTFKLIKQMQITAPLAVQQAAVSKALFDAIGGPDEANGVLTPSQIGAFGTV
                     MGGDTAKLYTELQKALGRTPVYLNTFGYDVGLITEAAMKNSDGSRQGIRDAIEKLKDL
                     PAVNGPVTFSPKDHTGQDYRSLTMAKLQHGVLTPAD"
     gene            8309..9574
                     /locus_tag="VKQ27_09405"
     CDS             8309..9574
                     /locus_tag="VKQ27_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY89190.1"
                     /translation="MLPPLRWVTILIPCLFAIPGARAADLPEPWAEYASDGGIDIRAV
                     TVPGMPCPKVVADGAVVPSKTRGQPDGAGGPYPVQVCVAHAATPPRAAAVDGVPVPVL
                     PATINRIVVVGDTGCRLKGTFVQDCNDPVKWPFATVARLAAARRPDLVIHVGDYHYRE
                     TPCPADRPGCAGSPYGDNWAVWQKDFFDPAAPLLAAAPWVMVRGNHEICSRGGHGWFR
                     LLDPHPDAVECTATTPPYALHIDTLNLLIFDGADADDSKADPEKVALYREQMQSLLTN
                     APPHSWLLTHHPVWALVEGEGAKQGDTLNATQQAAVRGLIPAGLNMVLSGHVHDFTSY
                     DFGPQRPSQLIIGEGGDVNDAIVQPVTGGIMLDGMKVRRAFAMPDWGYVVLRRVSEGW
                     AAAVYATNDSVLARCSLRGRNLTCRSVPH"
     gene            9571..10812
                     /locus_tag="VKQ27_09410"
     CDS             9571..10812
                     /locus_tag="VKQ27_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLY89191.1"
                     /translation="MTRDPTREAAFDLLSAVVDRRRPLEEALDGLPEMPPRDRAAGHR
                     LAAAVLRRMGTLDAVLEPFLRKEPPDPVRHVLRIGAAGLLLLETPSHAAVATAVALAR
                     SRGLAPFAGLVNAVLRRVSEAGAPAMAELDGPRLDTPAWLWASWGEAARAIAVAHQHE
                     APLDLTLRPGAAVPAGGDVLPSGSVRFPPGTRVAEIEGFEAGTWWVQDAAAALPARLM
                     GAQPGEHVADLCAAPGGKTGQLAAAGAAVVAVERDGARLARLCDNLRRWRLAAETVQA
                     DVLEWKAPQLFDAILLDTPCSATGTIRRHPDVPHTKRPRDVRALMETQDKLLIAAAAM
                     LRPGGRLVYAVCSLQPEEGAARIEAALQRGGVRHEPFTPDELVDLPEARTEQGFLRTL
                     PSMWPERGGMDGFFAARLVRV"
     gene            10861..11517
                     /gene="rpe"
                     /locus_tag="VKQ27_09415"
     CDS             10861..11517
                     /gene="rpe"
                     /locus_tag="VKQ27_09415"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLY89192.1"
                     /translation="MPASRVTIAPSLLAADFGRLREEVVAVEAAGADWLHLDIMDGHF
                     VPNISFGPPVMKSLRPLTKLPFDVHLMITPVDPYVEAFAQAGADHMLVHPEAGPHLHR
                     TLQLIRSHGKKAGVVLNPSTPPETIAWVMDLIDIILVMTVNPGFGGQAFLESQLLKIQ
                     ALRQMIDAAGRPIALSVDGGVAPETAPQVIAAGADTLVAGTAVFGTSDYAAAIAALRG
                     "
     gene            11514..>13063
                     /locus_tag="VKQ27_09420"
     CDS             11514..>13063
                     /locus_tag="VKQ27_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HLY89193.1"
                     /translation="MSGTDPRRWLRDARRVMARLPSLRISRVPDAPALPVRDPWPGDP
                     GRGARLLRGELEIGGGVRTLRPGAWGETAGSPVVDAMAHGFTWLRDLRALGTDAARLR
                     ARALVSEWVGGASSEALAHRPDVVGARITAWLGHYDFFAATADDVFRQKLMARLVSDA
                     RSLSAALPAEELDARGLTALKGLIAAAVALPEHAGFLTRALRFLPQEITRQVLPDGCH
                     VERSPAAQLAALQDLTEIRALLQAAQAAPPTALAGAIDRMAPALRVMRHGDGGLALFN
                     GSKEESSSLIDIALTQAGRGGRGPSSLKDGGFQRLQAGRSVVLVDCGVPPPPGVDRLS
                     HAGTLSMEMSVGRERLIVNCGAFPAGSGQWRDAMRATAAHSTLTIADVNSSELKTDGL
                     GRRPSVVEAQRQEANGAHWLEASHDGWKKLFGAVHRRRLYVAESGEDVRGEDVVEAPS
                     PQPFTLRFHLHPDVNASLQQDGEAVLLRLRSGSGWRLRADGARMSLEESVYLGGHEPR
                     RSEQVVLTG"
BASE COUNT         2062 a         3555 c         4875 g         2571 t
ORIGIN      
        1 gcatcgcggc gatcttgtcg gcgtctggcg cgtcgacggt gaactcgagg cggaggccgt
       61 ccggcgtttt catatacatc gacacgcaat agccgtggtc gatcttgcgt accggcacgt
      121 tggccgtctc cgtgcgctgc atgagttcgt cgaaggttgc ctgcgagact ttgaacgcga
      181 cgtgccagga gccaccgaca tcctgcagga tagcgcggtt cttttcccag gcttcctcgt
      241 cggcgaattg gaagaaggcg agcgcactgc cgtcgcccac ttcgtagaag gtgtggcagt
      301 agtcgacctc gcggccgact tccgggcgga aggtgcgttc gcaccaggtg gcgacgaggg
      361 gaatgccgag gatgtcctcg aagaactgac gattggtttc ctggtccttc accgcatagg
      421 catggtgatg caaccgcaac ggtagttcat tcaacatctc aatctcccgc ttgcatccga
      481 tccgagttcc cggttgtgtg ctgccatcgc acgggggagg ggacttgggc aagccacgga
      541 ttctgatcgc gggggctggc atcggcggca ttgtggccgc gctcgctctg ctgcaacgcg
      601 gcttcgaggt cgcgctgtat gagcaggcgg cagatctgcg cgagattggc gcgggcgttc
      661 agatctcgcc gaacgggtcg cgggtgctct gccatctggg gttgcaggcc gccatggagg
      721 cgatcgcctc ggtgccgaca gccaaggata tgcggctgtt cagtacaggt caggcctggc
      781 gcgtgcagga tctgggtgcg acggcggtgg ctcggttcgg gtcgccgtac tggctggtgc
      841 atcgcggcga tttccatgca gtgctggtgc gggcgttaga ggaacgggcg ccgggggccg
      901 cgcatgtggg cgcgcggtgc gtgctgttcg agcaggatgc aagcggcgtg acgttgctgc
      961 tgggaaacgg caaacgggtg cggggcgatg cgctgatcgg cgcggatggc gtgcattcgc
     1021 ggattcgcgg acaactcttc ggcgatgggc gggcgacctt caccgggtat atggcgtggc
     1081 ggggcgtggt gccgatggag cggttgccgc cgcatctgcg gcagccgcat gggaatacct
     1141 ggatcgggcc gttcggtcat gtggtgacgt atccgctgcg gcggggcgaa ctgctgaatt
     1201 tcgtcacagc gatcgagcgg gatgactggc tggtggagag ctggtcggag gcaggcacgg
     1261 ttgacgagtg ccggcgggat tttgcccggt ggcatccgga tgtattggcg atcgtggatg
     1321 cgatcgagac gccttacaag tgggcgatgc tggggcggca gccactggag cactggtcgg
     1381 tggggcgggt cagcctgctg ggggacgcgt gtcatccgac gctgcctttc ctcgctcagg
     1441 gcgcgaatat ggcaatcgag gatggcatgg tgctggctcg gtgcctggag gctttcgctg
     1501 atgtgccgga cgcattgcgg cactacgagg cagcgcggct ggagcggacg tcgcggattg
     1561 tgctcgggtc attggagaat gtgtcgcggt atcacaatcc acagttggcc gatccggcgg
     1621 aggcgcaagc tttcatggat cgggagttcg cgccgcgggc gatgggggcg cggtatgact
     1681 ggctgtatga gtacgatgcg ctgaaggtgg cggtgtgaat agttgaagac gtggatggtc
     1741 gcgccgcgag cggctatgac gattaatgcg aggccgcgac gggttgtgcg caagcatgat
     1801 gggagattga acgtgcgact gtctctggtc ctggctggat tcttgttggt tggtcacctc
     1861 gcgcgcgccg atgagccgga attcaatatc ggggtggtgg cgtcggtgac cgggcccttt
     1921 gcggcgccga cgaaggatac gttcgatggg ttcaatgcct ggatgaaggt gcacgggctg
     1981 ccgggtaaga agatcgtgct gcagacggtc gatgacgaga ccaacccggt gaatgcgtcg
     2041 aacgcgttcc gcaaactggc gtcagatcca aaggtttcgc tgatctacct gttcatcaat
     2101 agcaattcgg cgatggcggc gaagtccttc gcctcggagt tcaaggtgcc gatcatcacc
     2161 gggggtggtg cagatgcgct gggggttccg gccgatccgt ggatgttcaa ggtggcaccg
     2221 tcgaatcgcg actacatgat cgcgctgtcg gagtacatca agcgcaaggg ctatacgaag
     2281 gtcgcacatc tctattccac cgatacattc gggcaatacg atcatacgaa cctggagaag
     2341 ctggcgccgc aatatggcta caggctggtg gccaacgaga gtttcgcgat cgacgatacc
     2401 agcttcaacg cgcaattcac ccggatacgc gcggcgcagc ccgatctgat ctacagcagt
     2461 gcgagtgcgc gggccgcgat cctgtcattc aagcagtaca agcagcttgg catgacgacg
     2521 ccactggtgg tgacgggagc cgccgtcagc aaggcgttct tcgatgccat cggcggcccg
     2581 gcggaggccg atggaatgat gatgatgact cagcgaggct cgctcagcgc ggggctgggg
     2641 ggtgagtcgg cgaaatacta tgcagagcta aaggatgtgc tggggcgtac gccggtgttc
     2701 ttcaatgtgt tcggttttga cgtggggctg ataaccgcag gtgccgtgga gcattcggat
     2761 ggctcccggc aaggtattcg caatgcgctg gagaagctga aggacctgcc ggcgatcaat
     2821 ggcccggtga cctatacgcc ggttgatcac accgggcagg attcacgatc catcacgctt
     2881 gggaagctgg agaacggcaa gtctgtgccg gctgagtgat ggataccaca gtcttcctgc
     2941 aactgctgct gcctgggctc accaccgggt gcgtatatgc gctggtggcg ctgggatttg
     3001 tcctgtgcgc caacgtctcg ggtgtggtga atttcgcgca gggcgagttc gtcatgctgg
     3061 gtggcatgat ggcggcatgg ctgatcgggc ttggcgtgcc gttcgctctg gcgatcatcg
     3121 ccgcgacggc gttgggcgcg gctgcgggag cggcgcagga agtgctgacg ctggcgccgg
     3181 tgcgtcggca accgcacttc atccagatca ccacgaccgt gggatttgcc gtggtgttgc
     3241 gcggcatcac tcttatcgtc gcgggcaagg atgcgctgag cgtgccgggg ttttccggtg
     3301 acggcgtgtt cttcctgttt ggtgcgatct tgcctgtgca gagcttgtgg gtgtggggtg
     3361 ccacggtgct gctgctggtg gggacgttcg ggttcctgcg ttataccagc acggggcgcg
     3421 cggtgcgggc gtgttcgatc aatctgcagg cggcacggtt gatgggtgtg aatgccgagc
     3481 ggctgcgcgt gatcgtgttc gcggtgagtg gggcgacggg agctttggct ggcgtggtga
     3541 tcacgccgat cgtgctagcg agttgggatg cggggcttag ctacagcacc aaggggttta
     3601 tcggggcgat cattggcggg ttccgatcgc cgtcggcggc ggtggcggcg ggagtgttca
     3661 tcggactgct ggaggcgttg agcgccggtt atgtgtcgtc ggggtggaag gattttattg
     3721 tttacggtgt gttgttggcg tggctgctgg tgcagggcgg cgtgttcttg cgcgggcggg
     3781 tggatgcgac ggcaggcggg ccgtgaaata caaggccgcg attgccactg cgtctttcct
     3841 gcttgcggtg gtgctgtatc cactttgcta tcccgatagc tatcacctgg gtgttggcat
     3901 cagcgctggg gcgatggcgg tgagtaccgt tggtgtggtg ctgctgttgg gactggcgca
     3961 ccagcttgcg atcgggcagg cggcgttcag catgatcggc ggttacgcca acgcgatcct
     4021 gtgcgtgcgc tacggatggg atccgtttgt cgcgatggtg gcgggtgccg tgctggcgat
     4081 ggtgttggcc tatgccattg caacgccgct cttgaagctg cggggctttg tgctggcgat
     4141 ggcgaccttg gctctgcacc tgatgctgat cgtggtcgct gtgcaggtgg atttcaccgg
     4201 tgggccgatg ggtgttaccg gcattccaaa attcgcggtg ttcggtctgc cgctgagcaa
     4261 tgaccgctcg ttcttctggt tcatctggct gctggtattt ggcgcggttt ggattgggct
     4321 gcggatcgat cattcgtcca ttgggcgcgc gctacgggcg atcgcggcaa gcgatcaggc
     4381 ggcgggatcg gtcggcatcg atatcacgcg gcataaagtg cagatgttcg ttattggcgg
     4441 cgggatggcg agtgtttccg ggtcgctgct ggtgcactat ctgcgcgcga ccgatccgac
     4501 cgtgttcagc tttggctaca gcatcaacct gatcacgggg acgatcattg gcgggctgac
     4561 ctcgatctgg ggtggggcgc tcggggcggc gatcatcatt tttctgcgcg agctgctgcg
     4621 cgagctggag ttgccgttgt gggagtcggt gatcatgggc gcgctgaccg tgctggtcct
     4681 gatcgtgttt ccccggggca tcgcgggctt tgtcagccgt atctacgacc gggtggtggg
     4741 tgacgaggcg cctcggcgtg tggtggtgac ggaaccggga gccgtggcgt tgccggcgat
     4801 gagcgatccg ccagcgctgg tgctggatgg tgtgtcgcgc agtttcggca gcctgcgcgc
     4861 ggtgaatgcg gtggatttca ccgtgccggc aggtgcgatc accgcgctga tcgggccaaa
     4921 tggggcgggg aagacgacgc tgttcaatct gatcagcggg catctgcggc tcgacgcggg
     4981 ggtcgtacgg ttcgacgggc gcgagatcca ggctctggag ccccgggatg tggctcggct
     5041 ggggatcgga cgcacgttcc agaacctgca actgttcgac aatatgactg tgctgcagaa
     5101 cgtgatgtgc gggcatcatc ggttcggcgg tgccggcgcg gaacaggcag cgcgcgatac
     5161 gttggcgttt gtaggattgc agggagcgga gaggcatgag ccggcgtcgc tgccgttcgg
     5221 gcatcagcgg ctggtggaga ttgcacgggc gttggcgctg cggcctcgga tgctgctgat
     5281 ggatgagccg gcgtcggggc tgaacgatgc ggagacggag cggctgtcgg agctgatcgt
     5341 gcgggtggcg gcgttgggcg tgacagtgct gctggtggag cacgatatgc ggctggtgat
     5401 ggggttggcg gaccatgtgg tggtgatgga tcacggggag aagatcgccg aaggcgcgcc
     5461 ggaaccggtg cgcgccgatc ctgcagtgat tgccgcgtat cttggccagg agttgccggc
     5521 ccgtgctgct tgagctggat cgggtcagca gcggatatgg ccggtcgccg gtactgcatg
     5581 atgtgtcgct gactgtaggg gccggggaga tcgtcgcgct gattggcgcg aatggggcgg
     5641 ggaagagcac gctgctgaat acggtgatgg ggctggtggc gacctcatcg ggcgaggtcc
     5701 ggttcgacag gcgtgcgatc acgcggatgc cgacgccggc gatcgtgcgg gcggggatcg
     5761 ctcaggtgcc ggagcggcgg cagttgttcg gcacgatgtc ggtggaagag aacttactga
     5821 tgggagcgta cgcgtgcggc gattccagcg cgttggagga gcagcttgcg cgttttccca
     5881 tcatgcgaga gcggcggcat cagctggctc ggacgctgtc gggtggcgag cagcagatgg
     5941 tggcgctggc gcgcgcggca atgtcgcggc ctcgactgct gttgctggac gagccttcgc
     6001 tagggctggc tcccatgatc gtcgcgcgga tcatgcgcga gatcgcggcg ctgcgcgaag
     6061 cgggcggaac ggtgctcatc gtcgagcaga atgcgaatgc ggcactgggg atcgcggatc
     6121 ggggatacgt gctggagaat gggcggattg cgctggaggg gcaggcgggt gagctgctgg
     6181 tcaatccggc ggtgcaggag gcttatctcg gcgggcatgg cgggcattac gcgatcgagg
     6241 cacggatacg agcgaagaag atcgagctgt tggggcgtta gatatgcggt cgtgaatgac
     6301 tggcttcagt catttacttg catcgctcag acggaggatc tcatcaacga cgcgcgcgag
     6361 cggtgcggtg gcgtcgatga tgatggcgtc tttcggaatg tcttgcttcg tttgatgcaa
     6421 tcgcacagcg agttctcgtt cccctggttt tccaccgaag tcagttgggt ccagtgccac
     6481 tcgctcatca atccgcctgt tcatcgtgtc cacgtcgacg tccaggacaa acgcgccgtc
     6541 aaataagtcg atgaatttgg caaaatttct cgagccaccg cagaaaaagg tgaccgcctc
     6601 cttatggttg gcgacgaggg cttgcacctt ccccacatcc caaatctggt gctcagacat
     6661 ccaggtagcc gtcggtgcag ccatttccgg tgccgtcggt aggccggttt gtggatcgcc
     6721 tcgatacgcc aattcccggt cgccatgaat ggcctggtag ccacgtcgct gcaattctgt
     6781 acagaccgcc gttttgccgg cgccggaaac gccttcaatc agataatttc tcctgcccat
     6841 ggctcgataa tactcctttc aatgggatgg cgtctttggg ctgattcaag cgcagggtca
     6901 gtctagcgat cgaccgaccg gtaagactcg gcggagcgat gtcgttagag ggaccagcgg
     6961 tggagttgct ggtcaatccc gcattgtagg aggcgtatct cgacgggcat tgtggatatt
     7021 gcgcgatcga ggcgcggatc cggcagaaga agctggagct gttgggacgc taacgcgaag
     7081 aatgtcgcgg atgggcttgt cccggccatt cgtacaggca ctgtaccgca acggatggcc
     7141 gggacgagcc cggccaccac ggtttgggag gaaatgcgca tgctcgttcg tctggccgcc
     7201 gcattgtttg cggccttatc ggttatggct gtgcccgccg cggcacagtc cgaattcaag
     7261 atcggtgttg ttgcatcgct gaccggcggg ttcgcagcgg cttccactga tgagaacgct
     7321 ggcgtccagg catgggtgaa ggcgcgcgga ctgtccgggc ggaagatcgt gttcgagacg
     7381 ttggatgacg aaaccaaccc ggtgagcgcc atcaatgcgt tccgcaaggt tgccagcgat
     7441 ccttcagtgt cgctgatcta tgcgttgacc aacagcaact cggcgatggc gatcaagtcg
     7501 atcgcctcgg agttcaaggt gccgatcatt tccagtggcg ctgtcgagac gctgggcgta
     7561 ccggcagatc cttggctgtt caagatcgcg ccggggccgc gcgatatgat gatcgtgctt
     7621 tgtgaatacg cgaagaagaa gggctacacg cggttggcgt cgctgcacag caaggatgcg
     7681 ttcggccagg cggaggagac gtacatcaag gaactggcgc caaaatacgg gctgaagctg
     7741 gttgatgtgg aggagttcaa taccgacgac accaacttca cgccgcagat cgtcaaggtg
     7801 aaggcggctg atccacaatt gatctacaat ggcgcgacgg gacggccggg catcctgacg
     7861 ttcaagctta tcaagcagat gcagatcacc gcgccacttg cagtgcagca ggctgcggtc
     7921 agcaaggcgc tgttcgacgc gatcggcggg ccggatgagg cgaacggggt gctgacgccg
     7981 agtcagatcg gcgcgttcgg tacggtgatg gggggagata cggcgaagct gtataccgag
     8041 ttgcagaagg cgctcgggcg gacgcctgtc tatctcaaca cgttcggcta tgacgtgggg
     8101 ctgatcaccg aggcggcgat gaagaactcc gatggctcgc gccaaggcat tcgcgatgcg
     8161 atcgaaaagc tcaaggacct gccggcagtt aacggtccgg tgacattctc gccgaaggat
     8221 cacaccggac aggactaccg ctcgcttacc atggccaaac tgcagcatgg cgtgctgacg
     8281 ccggctgact gaaccgaagg ggagattgat gctgccgccg ctgcgctggg tcaccatcct
     8341 gatcccgtgc ctgtttgcga ttcccggcgc acgcgccgcg gacctgccgg aaccatgggc
     8401 ggagtacgcc agcgatggcg gcatcgatat ccgcgccgtg accgtgcctg ggatgccctg
     8461 ccccaaggtg gtggcggatg gtgcggtggt gccaagcaag acacgggggc agccggacgg
     8521 tgcgggtggg ccttacccgg tgcaggtgtg cgttgcccat gcggcgacgc cccctcgtgc
     8581 ggctgcggtc gatggcgtgc cggtgccggt gcttccggcc accatcaacc gcatcgtcgt
     8641 ggtcggcgat accgggtgcc ggctgaaggg taccttcgtg caggattgca atgatccggt
     8701 gaagtggccg tttgctacgg tggcgcgtct ggcggcggcg cggcggcccg acctggtgat
     8761 ccatgtcggc gattatcatt accgggagac gccatgtccg gccgatcggc ctggctgtgc
     8821 cggcagtccg tacggcgaca attgggcagt gtggcaaaag gactttttcg acccggccgc
     8881 gcctctgctg gcggcggcac cgtgggttat ggtgcgtggc aaccacgaaa tctgcagtcg
     8941 cggcgggcat ggctggttcc gcctgcttga tcctcatccg gacgccgtgg aatgcactgc
     9001 gaccacgccg ccttacgcgc tgcacatcga tacgttgaac ctgctgatct tcgatggtgc
     9061 ggacgctgac gatagcaagg ctgaccctga gaaggtcgcg ctttaccggg agcagatgca
     9121 gtcgctgctc acgaacgcgc cgccacactc gtggctgctg acccaccatc cggtctgggc
     9181 gctggtggaa ggcgagggcg ccaagcaggg cgacacgctg aatgccacgc aacaggcggc
     9241 agtccgcggc ctgatccctg cgggcctgaa tatggtcctc tcgggacacg tacatgattt
     9301 cacgagttac gatttcgggc cacaacgtcc gtcgcaactg atcatcggcg aaggcggtga
     9361 tgtgaacgac gccatcgtgc agccggtcac cggcggcatt atgttggacg gcatgaaagt
     9421 gagacgcgcg tttgcgatgc ccgactgggg ctatgtggtc ctgcgtcgtg tttcggaggg
     9481 ttgggctgcc gcggtctacg cgaccaacga tagcgtcctg gcccgctgtt ccttgcgtgg
     9541 tcggaatctt acctgccgct cggtcccaca ttgacgcgtg atcctacgcg tgaggcggcg
     9601 ttcgacctgc tgagcgctgt cgtcgatcgg cggcgtccgc tggaagaggc gctggacggc
     9661 ttgccggaga tgccgccacg ggatcgcgcg gcggggcatc ggctggcggc ggctgtgctg
     9721 cgccgcatgg gcacgctgga cgcggtgctg gagccgttcc tgcgcaagga gccacctgat
     9781 ccggtgcggc atgtgcttcg tatcggtgcg gccggattgc tgttgctgga gacgccgagc
     9841 cacgcggccg tggcgacagc ggtggcgtta gcgcgcagcc gcggattggc gccgtttgcc
     9901 gggctggtga atgcggtgct gcgacgtgtc tccgaggcgg gagcgccggc gatggcggag
     9961 ctggatgggc cgcggctgga tacgccggcg tggctttggg cgtcgtgggg cgaggctgcg
    10021 cgtgccattg cggtggcgca ccagcatgag gcgccgctgg acctgacatt gcgcccaggt
    10081 gcagcggtgc cggccggggg cgatgtgctg ccgtcaggat cggtgaggtt cccgcctggt
    10141 acgcgtgtgg cggagatcga agggttcgaa gccggtacgt ggtgggttca ggacgctgcg
    10201 gcggcgttac cggcgcgcct tatgggcgct caacccggag agcatgtggc ggacctctgc
    10261 gctgctccgg gcggtaagac ggggcagcta gctgcggctg gggccgcggt cgtggcggtg
    10321 gagcgtgatg gggcgcggct cgctcggctg tgtgacaatc tgcggcgctg gcgcctggcg
    10381 gcggagaccg tgcaggccga cgtactggaa tggaaagcgc cccagctctt cgacgcgatt
    10441 ctgctcgata cgccgtgcag cgcgaccggc acgatccggc gacatccgga tgtgccgcac
    10501 accaagcgcc cgcgcgacgt gcgtgctctg atggagacgc aggataagct gctgatcgca
    10561 gcggctgcca tgctgcggcc gggcgggcgc ctggtttacg cggtctgctc cttgcagcca
    10621 gaggaaggtg cggcccgcat cgaggcggcg ctgcaacgtg gtggcgtgcg gcatgagccg
    10681 ttcacgccgg acgagctggt agatttgccg gaagcccgca cggaacaggg ctttctgcgc
    10741 acgctgccaa gcatgtggcc ggagcgcggc ggcatggatg gatttttcgc ggcccggctg
    10801 gtgcgggtgt gactcagcgg cgccttgtgt ttcggcgggt gcgctgcata gatcgctgac
    10861 atgccagcct cccgagtcac tatcgccccc agcctgctcg ccgccgattt cggccgattg
    10921 cgcgaggaag tggtggcggt ggaagcggcc ggtgcggact ggctgcacct cgacatcatg
    10981 gacgggcatt tcgtgccgaa catcagcttc ggccctccgg tgatgaagag cctgcgcccg
    11041 ctgacgaaac tgccgttcga cgtgcatttg atgattacgc cggtcgatcc gtatgtggag
    11101 gcattcgcgc aggcaggcgc ggatcacatg ctggtgcacc ccgaggcggg gccgcacctg
    11161 catcgcacgc tgcagcttat ccgctcacac gggaagaagg ccggggtggt gctgaacccg
    11221 tctacgccgc cggagacaat tgcctgggtg atggacctga tcgacatcat cctggtgatg
    11281 acggtcaatc cgggattcgg cggccaggcg tttctggaat cccagttgct caagatccag
    11341 gcgctgcggc agatgatcga tgcggcgggc cggccaattg cgctgtctgt cgacggggga
    11401 gtggccccgg agacggcgcc tcaggtgatc gcggcgggag ccgatacgct ggtcgcgggc
    11461 actgcggtgt ttggcacgtc ggactatgcc gcggcgatcg ccgccctgcg cggatgagtg
    11521 gtaccgatcc tcggcgctgg ctgcgcgatg cgcgccgcgt catggcccga ctgccaagcc
    11581 tgcggatcag ccgggtgccc gacgcgcccg ctctgccggt ccgcgatccc tggcctggcg
    11641 atccgggacg cggtgcccgg ctgctgcgcg gcgagttgga gattggcggc ggcgtacgga
    11701 cgttacggcc cggggcgtgg ggcgagacag cgggatcgcc ggtcgttgat gcgatggcgc
    11761 acgggttcac ctggctgcgc gatctgcgcg cgcttggaac ggatgcggcg cggctgcgcg
    11821 cccgggccct ggtatcggag tgggtcggtg gggcatccag cgaggcgctg gcacatcggc
    11881 ctgatgtggt gggggcgcgg atcacggcgt ggctcgggca ttatgatttc tttgccgcga
    11941 ccgcagatga tgtgttccga cagaagctga tggcgcggct ggtatcggat gcgcgcagcc
    12001 tgtcagccgc cttgcccgcc gaggaactgg atgcccgcgg gctgactgcg ctgaaggggc
    12061 tgatcgctgc ggcagtggcg ttgccggagc atgccggctt cctgacccgg gcgctgcggt
    12121 tcctgccgca ggagatcacg cggcaggtgc tgccggacgg gtgccatgtg gagcgcagcc
    12181 cggcggcgca acttgctgcg ttgcaggacc tgaccgagat ccgggcgctg ttgcaggcgg
    12241 cgcaggcggc acctcctacg gcattggcag gggcgatcga tcggatggcg ccggccttgc
    12301 gggtgatgcg acatggtgac ggcggactgg cgctgttcaa cggcagcaag gaagagagca
    12361 gcagtctgat cgacatcgcg ctgacccagg ccgggcgcgg cgggcgcggg ccctcttcgt
    12421 tgaaggatgg cgggttccag cggttgcagg cgggacgcag cgtcgtgctg gtggattgcg
    12481 gggttcctcc gccaccgggg gtcgaccggc tgtcgcatgc tggcacgcta tcgatggaga
    12541 tgtcggtcgg tcgggagcgg ctgatcgtga attgcggtgc attccctgcc gggagcgggc
    12601 agtggcgcga cgccatgcgc gcaacggctg ctcactcgac tctgacgatc gcggacgtga
    12661 attcttccga gctgaaaacg gatgggctgg ggcggcggcc ttccgtcgtg gaggcgcagc
    12721 ggcaggaggc gaacggcgcg cattggctgg aagcgagcca tgatggctgg aagaagctgt
    12781 tcggcgcggt gcatcggcgg cgcttgtatg ttgcggaaag cggcgaggac gtgcgcggcg
    12841 aggatgtggt cgaggcaccg agcccgcagc cgtttacgct gcggttccat ttgcatccgg
    12901 acgtgaacgc cagcctgcag caggatggcg aggcagtgct gctgcggctg cgctcgggca
    12961 gtggctggcg gctgcgggcg gatggagcgc ggatgagcct ggaggagagc gtctatcttg
    13021 gggggcacga gccacggcgc agtgagcagg tggtgctgac cgg
//
LOCUS       DATGEM010000292         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1158974, whole genome shotgun sequence.
ACCESSION   DATGEM010000292 DATGEM010000000
VERSION     DATGEM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158974"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2645
                     /locus_tag="VKQ27_09425"
                     /pseudo
     CDS             43..2645
                     /locus_tag="VKQ27_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            2642..4273
                     /locus_tag="VKQ27_09430"
     CDS             2642..4273
                     /locus_tag="VKQ27_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLY89194.1"
                     /translation="MKNIGPRIPYGRSTSEYPFGTRSTRRKTKVGNLVFVRLPCISSV
                     ASVVQDLSVRCLAATTALALLAACTVGPDYVRPVADKPVAYKEIEGWKTAQPRDQELR
                     GKWWEAYNDPLLNGMLEQVSISNQNLVQAAAQFRQARALVQVARAGYLPSVSAGAGVT
                     RSQSSSGFISQNQNLTARGPSTSYSLSLDAAWEPDLWGRVRRTVEANEAGAQASAGDL
                     VALRLSIHAELAQDYFQLRALDTQKQLFDDTLTAYQRTLTLTQNQYTAGVAAKVDVIQ
                     AQTQLKTTQAQALDIGVQRAQLEHAIALLLGKPASDYSLPPASLAALPPAVPLGVPSA
                     LLERRPDIAAAERRVNAANAQIGVAEAAYYPSLTLSADGGYQSSRFSHWLTVPSRFWS
                     VGPALAQTLFDGGLRRAQTDQAIAAYDANVAGYRQTVLTGFKEVEDSLVALRILEEEA
                     AVQDEAVQNARQSVALTTNQYKAGIVSYLNVVTVQATALANERTAVDILNRRLAASVQ
                     LIKALGGGWKATDLPTPDELARGEPFPRKEAVPMR"
BASE COUNT          815 a         1451 c         1398 g          796 t
ORIGIN      
        1 tacgacgcgg cctccagcat ccttgcgcag aagctttcgc agatggacgg catcggccag
       61 gtttccgtcg gcggcagctc gctgcccgcc gtgcgcgtgg aaatgattcc gtcggcgttg
      121 cacaagtacg gcatcggcac cgaggacgtg cgcatcgcca tcgccacagc gaacgccaac
      181 cggcccaaag gcatggtgga agacgcatcg cgccactggc agatctatgc caacgaccag
      241 gcgcgcgaag cggcggagta tatgccgctg atcatcgcct atcgcaacgg ggccgcagtg
      301 catctcaccg acgtggcggc aatcaccgat tccgttcagg atctgcgcaa cgccggatcg
      361 gccgacggca agcccgcggt gctgatgatc ctgtaccggc agccgggtgc caacatcatc
      421 gagactgtgg atcgggtgcg ggcaacgctg ccgatgctgc gcgcctcgat tccgggcgca
      481 atcgacctcg cggtcgtgat agaccgcacg cctaccatcc gcgcgtcgct gcacgatgtc
      541 gaacgcacct tgctgatctc gattgcgctg gtggtgatgg tcgtgttcct gttcctgcgc
      601 aaggccagcg cgacgctgat tccgaccgtt gccgtgccgg tatcgctgct cggcacgttc
      661 ggtgcgatgt atctggccgg ctacagcctg aacaacctgt cgctgatggc cctaaccgtg
      721 gccaccggct tcgtcgtcga cgacgccatc gtcgtgctgg agaacatctg gcgccgcatc
      781 gaggcaggca tgccgccatg gaagccgctc tggtggggac gcgcgaggtc ggcttcactg
      841 tgttgtcgat gagcctgtcg ctgatcgccg tgttcatccc gatcctgctc atgggcggca
      901 tcatcggccg gttgttccgc gaattcgcgg tgacgctttc ggtcgccatc ctgatttcgc
      961 tggtgctttc gctcaccaca acgccgatga tggccgcgcg gctgttgcgc aagcagagcc
     1021 acgaccggga acccgggcgc cttgcgcgcg tcagccggcg cgcattcgac acactgcagc
     1081 atggctacca gcgttccctc gcgtgggcgc tgcggcacag cctgttcatg atgttgctgc
     1141 tggcggcgac cgtctgcctg aatgtctatc tctacatcat cgtgcccaag ggattttttc
     1201 cgcagcagga taccggccgg ctgagcggct tcatccaggc cgaccagggt atttcgttcc
     1261 aggccatgca gaagaagctc aatgatttca ttgaaatcgt gcgccaggac ccggcggtgg
     1321 aaaacgtcat cggttttacc ggcggcgggc agcgcaacag tggagcgatg ttcatcgcgc
     1381 taaagccgct ttcacagcgc aagatgtccg ccgacatggt gatcgcgcgc ctgcgcggca
     1441 aactcgtgcg cgaacccggc gccagtttgt tcctgcagcc ggtgcaggac atccgcatgg
     1501 gcgggcgcgc ggccaacgca cagtatcagt acacgctgca ggcggacgat ctcgccgagt
     1561 tgcgatcatg ggaacctcgc atacgtcagg cgctgatgca gttgccggaa ctggccgacg
     1621 tgaacaccga cagccaggac aagggtatac agacttcgct gacgatcgac cgcgatgccg
     1681 cctcgcggct gggcattgac ctgcgcaccc tcgacgcgac actgaacaac ctgttcggcc
     1741 agcgcgaggt atcggtcatc tataccgccc tgaaccaata tcgtgtggtc atggaagccg
     1801 ctccgcaata ctggcaaagc cccgaggcgc tcgatgacgt ctacgtgagc agcgccgccg
     1861 gtaaacaggt gccgttgtcg gccttcgcaa actacggccc gaccagcacg ccgttggcgg
     1921 tgaaccatca ggggcagttc gtcgcttcga cgatctcatt caatcttccg gtcggtgttt
     1981 cactgtcgga tgcgacacgt gcgatcagcg ataccatcaa tcgcatcggc gtaccgactt
     2041 cgattcaagg cagtttccag ggcacggcgc gcgccttcca ggcgtcgctc aacagccagc
     2101 cctggctgat cctgacggcg ttgctgacgg tatacatcgt gctcggcatg ctctacgaga
     2161 gtttcgttca cccgatcacc atcctgtcga cgctgccctc cgcgggggtg ggcgcgctgc
     2221 tcgcgttgct cgcctgcggc agcgagttca gcatcattgc gctgatcggc gtgatcctgc
     2281 tgatcggcat tgtcaagaaa aacgcgatca tgatgatcga cttcgcactc gacgcggagc
     2341 gcaggctggg catggcgccc gagcaggcga tctaccaggc ctgcctgctg cgattcaggc
     2401 cgatcatgat gaccaccatg gccgccctgc tcggcgctgt gccgctcgcc atcggtttcg
     2461 gtgacggtgc cgaactgcgc cgcccgctcg gcatctcgat cgtgggcggc ctgatcgtca
     2521 gccagatgct gactttgtac accacaccgg tggtctatct ctatctggac cgcttccgcc
     2581 tgtggtgcaa acgcgctatc gcccggcgca cgccgcctgt cggaacttca ggaagtccag
     2641 catgaaaaat attgggccac ggatacccta cgggcgaagc acgtcggagt acccttttgg
     2701 aacacggagt acgcggagaa aaaccaaagt ggggaatctc gttttcgttc gcttgccttg
     2761 tatttcctcc gtggcctccg tggttcagga tctatccgtt agatgcctcg ccgctacgac
     2821 cgctctggca ttgctcgccg catgtacggt cggcccggat tacgtgcggc cggtcgccga
     2881 caagcccgtg gcgtacaagg aaatagaggg ctggaaaacg gcgcagccac gcgaccagga
     2941 actgcgcggc aagtggtggg aagcgtacaa cgacccgctg ctgaacggca tgctggagca
     3001 ggtcagcatt tccaaccaga acctggtcca ggccgcggca caattccggc aggcgcgagc
     3061 gctggtgcaa gtggcgcgcg cgggttatct gccgagcgtc tcggccggcg ccggggtaac
     3121 gcgttcgcaa tcgtcctccg gcttcatcag ccagaaccag aatctgactg cgcgcgggcc
     3181 gagcacaagt tactcgcttt cgcttgatgc ggcctgggaa cccgatctgt ggggccgcgt
     3241 gcgccgcacc gtggaagcga acgaagccgg cgcacaggcc agtgccggcg acctggtcgc
     3301 attgcgcctg tcgattcacg cggaactcgc gcaggactat ttccagctgc gcgcgctgga
     3361 cacgcagaag cagttgttcg acgacaccct cacggcttat cagcgtacgc tcacgctgac
     3421 gcaaaatcag tacaccgccg gcgtcgccgc caaggtcgac gtgatccagg cgcagacgca
     3481 gctgaagacc acgcaggcgc aggcgctcga catcggggtg cagcgcgcgc agcttgagca
     3541 cgcgattgcc ttgctgctcg gcaagccggc ttccgactac tcgctgccgc cggcgtcgct
     3601 ggccgcgctg ccgcccgccg ttccgttggg cgtgccctcc gccttgctcg aacgccggcc
     3661 ggacatcgcc gcggccgaac gccgcgtcaa cgcagcgaat gcgcaaatcg gcgtcgccga
     3721 agccgcctac tacccgtcgc tcacgctgtc cgcagacggc ggttaccaga gttcgcgttt
     3781 ctcccactgg cttacagtac ccagccgctt ctggtccgtg ggacccgcgt tggcacagac
     3841 cctgttcgac ggcggcctgc gacgcgcaca gaccgaccag gcgattgccg cctacgacgc
     3901 caacgtcgcc ggctaccgcc agacggtgct gaccggcttc aaggaagtcg aggacagcct
     3961 cgtggcattg cgtatcctgg aagaagaagc agcggtccag gacgaagcgg tgcagaacgc
     4021 ccgccagtcg gtggcgctga ccaccaacca gtacaaggcc ggcatcgtca gctacctgaa
     4081 cgtggtgacg gtgcaagcca ccgcgttggc caacgaaagg accgccgtgg acatcctgaa
     4141 ccggcggctg gcggcaagcg tgcagttgat caaggcactc ggagggggct ggaaagcgac
     4201 cgacctgccg acaccggacg aactggcgcg cggcgagccc tttccgcgca aggaagccgt
     4261 gccgatgcga taggcagatg gagcaagcgc cccagggcag tcgcaaccgg agggtgccgg
     4321 atgaaaaacc gaagcgcgac aaagcgactg ccggatggga ccggcatcgc caaagcagta
     4381 aggaggattc ttcgccatga cgtcccgccg cgcttttcta acggattcgc atgcgacccg
     4441 gatcgtgccg gtcgccattg
//
LOCUS       DATGEM010000293         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1369517, whole genome shotgun sequence.
ACCESSION   DATGEM010000293 DATGEM010000000
VERSION     DATGEM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369517"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1501
                     /gene="glnA"
                     /locus_tag="VKQ27_09435"
     CDS             68..1501
                     /gene="glnA"
                     /locus_tag="VKQ27_09435"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HLY89195.1"
                     /translation="MARKPAGAASGVGKVLEMLTEHSVEYVDLRFTDPRGKWQHTAQH
                     VSTIEEDTFRDGIMFDGSSIAGWKAINESDMILMPDPDTAVMDPFAAKPSLVLICDIV
                     EPSTGQPYNRDPRSTAKKAEAYVKASGVGDAVFFGPEAEFFVFDSVKYGTGGNYGIYQ
                     LESQEGPQASLKDYPEGNMGHRPSVKGGYFPVPPVDSESDLRAEMLSTMGEMGVVIEK
                     HHHEVAQSQHELGMKFGPMVKMADALQIYKYCIHNVAHSYGKTATFMPKPIYGDNGSG
                     MHCHQSIWKAGKPLFAGNSYADLSETCLYYIGGIIKHAKSLNAFTNPTTNSYKRLIPG
                     FEAPVLLAYSARNRSASCRIPYATNPKAKRVEVRFPDAAANPYLAFAAMLMAGLDGIK
                     NKIHPGDPMDKDLYDLPPEELKGIPTVCGSLREAIGALEADHEYLLAGDVFSMDQIES
                     YIELKWGEVYRFEHTPHPVEFEMYYSV"
     gene            1724..2062
                     /locus_tag="VKQ27_09440"
     CDS             1724..2062
                     /locus_tag="VKQ27_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLY89196.1"
                     /translation="MKLIMAIIKPFKLDDVREALTPLGVQGLTVSEVKGFGRQKGQTE
                     IYRGAEYHVNFLPKVKIEVVVPADLTDQVVEAITKSAHTGKIGDGKIFVIDIERAVRI
                     RTGEMDVAAL"
     gene            2149..>2985
                     /locus_tag="VKQ27_09445"
     CDS             2149..>2985
                     /locus_tag="VKQ27_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HLY89197.1"
                     /translation="MPAMADDAPPKLDSGDTAWMLASTALVLMMTVPGLALFYGGMVR
                     KKNILATLMQSFAITCIVTIVWCVAGYSLAFTPGNEYVGDLSRFMLNGIADHIAKGND
                     TQGFVLQSGIKGVAPMATTIPETVYMMFQMTFAIITPALIVGAFADRMKFSALCIFMT
                     LWSLLIYSPVAHWMWAPSGWSAQNGQLDFAGGTVVHINAGIAGLVCALVLGKRVGYGT
                     DNMAPYNLAYSVIGASLLWVGWFGFNAGSAVAANGRAGMAMSVTQIATAAAALGWMFA
                     EWI"
BASE COUNT          594 a          913 c          921 g          557 t
ORIGIN      
        1 gccgccacgg gatgcggtcg catggtgcgg cccgcaccaa ttgtgactag cgacggggta
       61 atcatacatg gcgagaaagc ctgcaggcgc ggccagcggc gtcgggaaag tgctggagat
      121 gctgacggaa cattccgtgg aatacgtgga cctgcggttc accgatcccc gcggcaagtg
      181 gcagcacacc gcccagcatg tttccaccat tgaggaagac accttccgcg acggcatcat
      241 gttcgacggc tcttcgattg ccggttggaa ggcgatcaat gagtccgaca tgatcctgat
      301 gccggatccc gataccgcgg tgatggaccc gttcgcggca aagccaagcc tggtgctgat
      361 ctgcgatatc gtcgagccgt ctaccggcca gccctataac cgtgaccctc gctccaccgc
      421 caagaaggcc gaggcctatg tgaaggcgtc aggcgtcggc gatgccgtct tcttcggtcc
      481 ggaagccgag ttcttcgtgt tcgacagcgt gaagtacggc accggtggca actacggcat
      541 ctatcagctc gaaagccagg aagggccgca ggccagcctg aaggactacc cggaaggcaa
      601 catgggtcat cgcccgtcgg tcaagggcgg ctacttccca gtcccgccgg tcgacagcga
      661 aagcgatctg cgcgccgaaa tgctctcgac catgggcgag atgggcgtgg ttatcgagaa
      721 gcaccaccac gaagtcgcgc agtcgcagca cgagctcggc atgaagttcg ggccgatggt
      781 gaagatggcc gacgcgctgc agatctataa atactgcatc cacaacgtgg cacacagcta
      841 tggcaagacc gcgacgttca tgccgaagcc gatctatggc gacaatggct ctggcatgca
      901 ttgccaccag tcgatctgga aggccggcaa gccgctgttc gcaggcaatt cttacgccga
      961 tctgtcggag acctgcctct actacatcgg cggcatcatc aagcatgcaa agtcgctgaa
     1021 cgcgttcacc aacccgacca ccaacagcta caagcgcctg atccctggct tcgaggcgcc
     1081 ggtgctgctg gcctattcgg cgcgtaaccg ctctgcctcg tgccgcatcc cgtatgcgac
     1141 caacccgaag gcgaagcgcg tcgaagtccg gttcccggac gccgctgcca atccgtatct
     1201 cgcgttcgcc gcgatgctga tggccggcct ggatggtatc aagaacaaga tccatcctgg
     1261 tgatccaatg gacaaggacc tttacgacct gccgccggaa gagctgaagg gcatcccgac
     1321 agtgtgcggc agcctgcgcg aggcgatcgg tgccctggaa gccgaccatg agtatctgct
     1381 ggccggcgat gtgttcagca tggaccagat cgaaagctac atcgaactga agtggggcga
     1441 ggtatatcgc ttcgaacaca cgccgcaccc ggtagagttc gagatgtatt actccgtctg
     1501 atcaccaaca ccttttccct gcccagcgcc cccgcctttc caggaaaggc gggggcgctt
     1561 cgcttttggc cgacagctgc tcgtttcttg agcaatacac cgccgcggct cgaaaaaacc
     1621 tgcgagaccc ggcgctccat cgtcgcggca tgattcttga tagacccctg atgaccgttc
     1681 cgccgtaggg gcggtgacgc gagagcaacg ttaggggcag tgcatgaagc tgatcatggc
     1741 catcatcaag cccttcaagc tcgatgacgt gcgtgaggcg ttgacgccac ttggcgtgca
     1801 gggtctcacc gtctccgagg tgaaggggtt cggccggcag aaaggccaga ccgaaatcta
     1861 ccgcggtgcc gaataccacg tgaactttct gcccaaagtg aagatcgaag tcgtggtgcc
     1921 ggccgatctg accgaccagg tggtcgaagc gatcaccaag tcggcgcata ccggcaaaat
     1981 cggcgacggc aagatcttcg tcatcgacat cgaacgcgcc gtgcggatcc gcaccggcga
     2041 aatggatgtc gcggcactct aaagaccaaa caaggggaaa tcataactat ggttcgacgc
     2101 cgacttgccg ttgggctggg cgctgtgctg ccgctcgtct tgctggcgat gccagcgatg
     2161 gcggacgacg cgccgcccaa gctcgactca ggtgacactg cctggatgct tgccagcact
     2221 gcacttgtcc tcatgatgac ggttcctggc ctggcgctgt tctatggcgg catggtccgg
     2281 aagaagaata ttctcgccac tctgatgcag tcgttcgcca tcacctgcat cgtgaccatc
     2341 gtctggtgcg tggccggcta cagcctcgca ttcacgccgg gcaacgaata tgtcggcgat
     2401 ctgtcgcgct tcatgctgaa cggcatcgcc gatcacatcg ccaaaggcaa cgacacgcag
     2461 ggcttcgtgc tgcagtcggg catcaagggc gtggcaccga tggccacgac gatccctgag
     2521 accgtctaca tgatgttcca gatgacgttc gcgatcatca cgccggcgct gatcgtcggt
     2581 gccttcgccg atcggatgaa gttctcggcg ctgtgcatct tcatgacgct gtggtcgctg
     2641 ctgatttatt cgccggtcgc gcactggatg tgggcgccga gcggctggag cgcgcagaat
     2701 ggccagctcg acttcgccgg cggcaccgtg gtgcacatca acgccggtat tgcgggactg
     2761 gtctgcgcat tggtactggg caagcgcgtc ggctacggca ctgacaacat ggcgccgtac
     2821 aacctggcct attcggtgat cggcgcatca ctgctgtggg tcggctggtt cggcttcaac
     2881 gccgggtccg ccgtggcggc gaacggccgc gcgggcatgg ccatgtcggt gacgcagatc
     2941 gccaccgccg ccgcggcact gggctggatg ttcgcggagt ggata
//
LOCUS       DATGEM010000294         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1117558, whole genome shotgun sequence.
ACCESSION   DATGEM010000294 DATGEM010000000
VERSION     DATGEM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117558"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VKQ27_09450"
     CDS             <1..302
                     /locus_tag="VKQ27_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HLY89198.1"
                     /translation="EAFNNLLPTADAIDFRAHAASLGANAEQAEGIAELETALRRALT
                     ADKTSVVVIRTDPHIATAAGGHWWDVGVPEVSARPQVNAARAEYEAKRVEQRLGT"
     gene            complement(197..>2156)
                     /locus_tag="VKQ27_09455"
     CDS             complement(197..>2156)
                     /locus_tag="VKQ27_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89199.1"
                     /translation="GKVDYTLRCLASIAAYPPRASLEVIVVDDAADDGSTECLAAIAG
                     IRLIINPKNLGYLRACNAAARVAAGEYLLFLNNDTQVLAGWLDPLLTPFRDQRNVGAV
                     GSKLLYPDGLLQEAGCIIWNDGSGWNYGRLDDPGRPAYNYLREVDYCSGASLMVPRAL
                     FSRVGGFDEQYAPAYCEDSDLAFRLREVGYKVIYQPRSRIVHYEGVSHGRDTSSGIKA
                     YQVRNVRAFNTRWRDVLARDHLPNGAHVMRARDRAGQRDIILVIDHYVPEPDRDAGSR
                     SATCMIRALLQDGLVVKFWPQNLRYSPGYTDALQDLGVEVAFGGDGNAFRSWLAENGA
                     DIRYALVCRPHVALDYLPELSRYPNIGTLFYGEDLHFRRMRMQARVEDDRSIARQADH
                     MERQERAVWRSVDVVLYPSDDESATVTAMEPGVNARTLLPYCFADFGVSRAPPTEPTL
                     LFVGGFAHLPNQHAAQWFVEFVLPRVRAQVPDAKLAIVGSNPSPEVCALAGQSVSVTA
                     NVSDERLRELYRTSRVAVVPLRYGAGVKLKVVEALREGLPLVTTPVGAQGLPGLGDIV
                     SAHDDATGMADAICRLLTDDVLWQERCAAQIAFAAARFSEAAFRESMTAALAQVPSRC
                     STRFASYSARAALTCGRAETSGTPTSHQ"
BASE COUNT          425 a          713 c          668 g          350 t
ORIGIN      
        1 gtgaggcatt caacaacctg ctgccgaccg ccgacgccat cgactttcgc gcccatgccg
       61 caagcctagg tgccaatgca gaacaggctg aggggatcgc cgaactggaa acggcgctcc
      121 ggcgcgcgtt gactgcggac aagaccagtg tagtggtgat ccgcactgac cctcacatcg
      181 cgaccgctgc cggtggtcat tggtgggacg ttggcgtgcc ggaagtctca gcccggccgc
      241 aggtcaacgc ggcacgcgcc gaatacgagg caaaacgcgt cgagcagcgg ctcggcacct
      301 gagccagtgc cgctgtcatg ctttcgcgga aggcggcttc cgagaaacgc gctgccgcga
      361 aggcaatctg cgccgcacag cgttcctgcc atagcacgtc atcggtcagc aggcggcaga
      421 tcgcgtcggc catacccgtc gcatcatcat gggcggagac gatgtcaccc aatcccggca
      481 gcccctgtgc accaaccggc gtggtaacca gtggcaggcc ttcacgcagc gcctccacca
      541 ctttcagctt cacaccggcg ccatagcgca gcggcaccac cgcaacccgc gatgtccgat
      601 aaagttcgcg cagccgttcg tcgctgacgt tggcggtgac gctgactgat tgcccggcca
      661 gcgcacacac ctccggcgaa ggattggaac caacgatcgc cagcttcgca tccggcacct
      721 gggcccggac ccgcggcagc acgaactcga cgaaccactg tgcggcatgc tggttcggca
      781 gatgcgcgaa cccgccgacg aacagcaaag tgggctccgt tggcggcgcg cgggataccc
      841 cgaaatcggc aaagcaatac ggcaacagcg tccgcgcatt gacgcccggc tccatcgcgg
      901 tgaccgtcgc gctctcgtcg tccgacggat agagcaccac atcaaccgag cgccagacag
      961 cacgttcctg ccgctccatg tgatcggctt gccgagcgat gctgcgatcg tcttccactc
     1021 gcgcctgcat gcgcatccgg cggaagtgca ggtcttcgcc gtaaaacagt gtgccgatat
     1081 tcggataacg gctgagttcc ggcaggtaat ccagtgccac gtgcggccgg cacaccagcg
     1141 cataacggat atcagcgccg ttttccgcca gccaggagcg gaacgcgttg ccatcgccac
     1201 cgaacgcgac ctccacgccc aggtcctgca aggcatcggt atagccggga ctgtaacgca
     1261 ggttctgcgg ccagaatttc accacgagcc cgtcctgcaa cagggcacgg atcatgcacg
     1321 ttgcggagcg tgaaccggca tcgcggtccg gttccggcac gtaatgatca attaccagga
     1381 tgatgtcgcg ttggcctgcc cggtcacggg cccgcatgac atgcgcaccg ttcggcaggt
     1441 gatcgcgcgc cagcacatcg cgccatcgcg tattgaaggc acgcacgttg cggacctgat
     1501 acgccttgat gccgctgctc gtatcgcgac catggctgac gccttcgtag tgtacgatgc
     1561 gcgagcgcgg ctggtagatc accttgtagc caacctcgcg caagcggaac gccaggtcgc
     1621 tgtcctcgca gtaggcaggc gcatactgtt cgtcgaagcc gccaacgcgg gagaatagcg
     1681 cgcgcggcac catgagcgag gcacctgagc agtaatcgac ctcgcgcaga taattgtagg
     1741 cggggcgacc gggatcatcg agccgcccat aattccagcc cgatccgtcg ttccagatga
     1801 tgcagccagc ttcctggagc aagccgtccg gatacagcag cttggagccg acggcgccca
     1861 cattcctttg gtcacggaaa ggggtcagca acggatcgag ccagcctgcg aggacctgcg
     1921 tgtcgttgtt gaggaacagc aggtattcac cggcggccac acgcgccgcc gcattgcagg
     1981 cccgcaggta gccaaggttc tttggattga tgatcagccg gatgcctgcg atggcggcga
     2041 ggcactcggt agagccgtcg tccgccgcgt catcgacgac gatgacttca agcgacgccc
     2101 gcggcggata cgctgcgatc gatgccaggc agcgcagggt gtaatcgacc ttgccg
//
LOCUS       DATGEM010000295         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1405729, whole genome shotgun sequence.
ACCESSION   DATGEM010000295 DATGEM010000000
VERSION     DATGEM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405729"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..925)
                     /locus_tag="VKQ27_09460"
     CDS             complement(8..925)
                     /locus_tag="VKQ27_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89200.1"
                     /translation="MATPVATAYVPPRSFAASGGARYRLIKVWREARRIPLLPSAVIV
                     MFVIFAVFAPLVSPYDPTQIALVQKLKPPFFIEGGGIAHLLGTDNLGRDVLARLIWGS
                     RVSLSVTAAVVVISSIVGLLIGLAAGYVGGRADDFLMRVADGSIAFPAVLIALLFAVS
                     LGPGVTTVIIALSVLSWANYARIVRGEVLRMRQLEFIVGARVIGCSPLRVMVAHLFPN
                     LVDVWVVMITLQVGLIVLSEAALGFLGAGVPPPTPSWGSLVSDGRNFVDTAWWISFFP
                     GLAIGLLVLSVNYLGDWLRDRMDPQLRHR"
     gene            complement(930..1844)
                     /locus_tag="VKQ27_09465"
     CDS             complement(930..1844)
                     /locus_tag="VKQ27_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89201.1"
                     /translation="MGRFLLNRVIQSIVTILVISMVVFLMARATGDPVTLLASDKATA
                     QDIAVLKQQLGLDQPVPVQYWVFLTNAVRGNLGKSLADKGDVAQLIGERLPNSAVIGL
                     PALLLSTVIGMAMGVVAAVKRDTWVDKSMRVIAVLGQSMPAFWVSIMAIFFFSVVLRV
                     LPTSGMGTPDHYILPIAVLTFFALPMRMRLMRSSLLEIMGTEYVKQARAKGLSESAVV
                     WRHAVRNALSPQLTSFGLTLAFAVTGAVLVETVFSWPGLGLLAYQAMLARDYPLIQGT
                     VLLVAVLTIGANLLVDMLYAVVDPRIRY"
     gene            complement(1864..2622)
                     /gene="yqeC"
                     /locus_tag="VKQ27_09470"
     CDS             complement(1864..2622)
                     /gene="yqeC"
                     /locus_tag="VKQ27_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HLY89202.1"
                     /translation="MEQRLAQALGIGEREVVTIVGGGGKTTTMFRLGRELRAAGAGVV
                     LTTTTHIFAPAPEADLETVVEQDAARSLAKLREALGRGHLPVVGTDIKPDGRLGGVSS
                     EVADYFAEHPDLAYVIVEADGSAHKPFKAPLEYEPVVPASTTLLVAVVGVDALGMPLD
                     AEHIHRPLRVAELSGAELNQPLSAEAIASVLVHPLGPLRDAPHGARVLVLLNKADSPE
                     RIAAARAIASELRAVNGPPVIIGAVASAALLDPI"
     gene            2637..3485
                     /locus_tag="VKQ27_09475"
     CDS             2637..3485
                     /locus_tag="VKQ27_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY89203.1"
                     /translation="MPTIDAQVHAYERNHAGRPWVGHLQGPPEVTGDQMVAAMDAVGV
                     DGAILISPFSLYRYDASYALEVYAAHPDRFGLIKPVDPTDPAVAETIADWAATAGTVG
                     VRIMLNRELPADPADPGINRVLATARQHSLPVNMLAWGRLDQARELAARNPDTQVVID
                     HVGLQQPFLPPPPAEPFAELPRVLALAAQPNIAIKITGACTLSHEPFPYRDIWDPLGR
                     IFDAFGLERCLWGTDWTRAVALLTYEQGVEAFRVSDRLSDSDRAMLMGGALERVYNWA
                     PSRLKA"
     gene            3585..>3882
                     /locus_tag="VKQ27_09480"
     CDS             3585..>3882
                     /locus_tag="VKQ27_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLY89204.1"
                     /translation="MMQHRRPRYIGLDVHRATIAVAIAEEEGSPSSYGTIANDPSAVR
                     KLMTRLGGKDVDLRVAYEAGPTGYALHRQLSKLGIQCIVVAPSLIPVRPGDKVKT"
BASE COUNT          780 a         1305 c         1198 g          599 t
ORIGIN      
        1 gaaggggtca gcggtggcgc aactgcgggt ccattcggtc ccgcagccag tcacccagat
       61 aattgaccga cagcaccagc agcccaatcg ccagccctgg aaagaaggag atccaccagg
      121 ccgtatccac gaaattgcgg ccatccgaaa ccagcgagcc ccaagacgga gtcggcggcg
      181 ggacgccggc tcccaggaac cccaacgccg cttccgatag gacgatcaag ccaacctgca
      241 gcgtgatcat caccacccac acgtcaacca gatttgggaa caaatgcgcc accatcacgc
      301 gcagcggcga acagccaatg acccgcgcac cgacaatgaa ttccagttgc cgcatgcgca
      361 gcacctcgcc ccggacgatg cgcgcataat tggcccagct cagcaccgaa agcgcaatga
      421 tgaccgtggt aacccccggc ccaagactga ccgcaaacag cagcgcaatg agaaccgcgg
      481 gaaacgcgat gctgccatcc gcgacgcgca tgagaaaatc gtcggctcgg ccaccgacgt
      541 agcccgccgc cagaccaatc aacaggccaa caatgctcga aatcaccacc accgctgcgg
      601 tgacgctgag cgacacccgc gagccccaga tcagccgagc caggacatca cgccccagat
      661 tgtcggtgcc aagcagatgc gcgatgcccc cgccctcgat aaagaacggc ggcttcagct
      721 tctgcacgag cgcaatctgg gtcggatcat agggcgacac cagcggagcg aacaccgcga
      781 aaatgacgaa catcacaatg acggcgctcg gcagcagcgg aattcggcgc gcttcccgcc
      841 atacctttat cagccggtat ctggcaccgc ccgacgcggc aaaacttcgc ggtgggacat
      901 aggccgtcgc gaccggtgta gccattgcgt cagtacctga tgcgcggatc gaccaccgcg
      961 tacagcatgt cgaccagcag gttcgcgccg atcgtcagga ctgccaccag caagaccgtt
     1021 ccctgaatga gcggatagtc gcgtgccagc atggcttgat acgccagcag acccagaccc
     1081 ggccaggaaa atacggtttc gaccagcacg gcgccggtga ccgcaaatgc cagcgtcagc
     1141 ccaaaggagg tgagttgcgg agagagcgcg ttgcgcactg catgccgcca gaccaccgcc
     1201 gactccgaca gacctttcgc gcgcgcttgc ttcacatatt cggtacccat gatctccagg
     1261 agcgacgagc gcatcagccg catgcgcatg ggcaacgcga agaaggtcag caccgcgata
     1321 ggcaagatgt aatggtccgg tgtgcccatc cctgaggttg gcaacacccg cagaacaact
     1381 gagaaaaaga agatggccat gatcgacacc cagaaggcgg gcatcgactg gcccaggacg
     1441 gcaatgaccc gcatcgactt gtcgacccac gtgtcgcgct tgaccgccgc gacgacaccc
     1501 atggccatgc cgatcaccgt gctcagcagc agcgcgggca ggccaatcac cgccgagttc
     1561 ggcagtcgct cgccgatcag ctgagccacg tcgcctttgt cggccagcga cttgcccaga
     1621 ttgccgcgga cggcgttggt caggaacacc cagtactgaa cgggcacggg ctgatcgagg
     1681 ccgagctgtt gcttcagcac ggcgatgtcc tgggccgtgg ccttgtcaga ggcgagcagc
     1741 gtgaccgggt cgccggtggc gcgcgccatc aggaacacga ccatcgagat caccaggatg
     1801 gtgacgatcg attggatcac ccgattcagc aaaaagcggc ccatgccacc gtcccaaacg
     1861 cgatcatata ggatcaagca gcgccgcgct cgcaaccgcg ccgatgatca ccggcggtcc
     1921 gttgacagca cgcagctcgc tcgcgatcgc gcgtgccgct gcgattcgtt cgggcgagtc
     1981 ggctttgttc agcagcacca gcacacgcgc gccgtgcggc gcatcccgca gtggacccag
     2041 tgggtggacc agcacgctcg caattgcttc agcactgagc ggctgattca gttccgctcc
     2101 actcagctcc gccacgcgta atgggcggtg gatatgctcc gcatccagag gcatgccaag
     2161 cgcatccact ccaacgaccg ccaccagcag cgtggtggag gcgggcacca ccggttcgta
     2221 ctcgagcggg gccttgaatg gcttgtgagc ggaaccatcg gcctcgacga tgacgtaggc
     2281 caggtccggg tgctctgcaa aatagtccgc gacctcggac gagactccgc ccagcctgcc
     2341 gtccggtttg atgtcggttc cgacaactgg gagatgccca cggccgagtg cttcgcgcag
     2401 ctttgccagg ctccgcgccg cgtcctgctc gaccaccgtc tccaggtcgg cctcgggcgc
     2461 tggtgcaaag atgtgcgtcg tggtggtgag caccactcca gctcccgccg cgcgcagctc
     2521 gcgcccgagt cggaacatgg tcgtcgtttt gccgccacca ccaacgatcg tcaccacctc
     2581 gcgctcgccg attccgagcg cctgcgccag ccgctgttcc atgcgaagat tgtgcggtgc
     2641 caacaatcga cgcgcaggtc cacgcctacg aacgcaacca cgccggccgg ccctgggtcg
     2701 gccacctgca aggtccgccc gaggttaccg gcgatcaaat ggtcgcggcg atggacgcag
     2761 tcggcgttga cggcgcgatc ctgatctcgc cgttttcgct gtatcgctac gacgccagct
     2821 acgcgctcga ggtctacgcc gcgcatcctg accgcttcgg actgatcaaa ccggtcgatc
     2881 ccaccgaccc cgcggtcgcc gagaccatcg ccgactgggc ggcaaccgcc gggacggtcg
     2941 gggtgcgcat catgctgaac cgcgagctgc cggccgaccc agccgatccg ggcattaacc
     3001 gcgttctggc aactgccagg cagcactcgt tgccggtgaa catgctcgcc tggggccggc
     3061 tcgatcaggc gcgcgagctg gccgcgcgca atcccgacac gcaggtcgtg atcgaccacg
     3121 tcggcctgca gcagccattc ctgccgccgc cgcccgcgga gccgtttgcc gagctgccca
     3181 gggtcctggc gctggctgcc cagccgaaca tcgccatcaa gatcaccggc gcgtgcacct
     3241 tgtcgcacga gccctttccg tatcgcgata tctgggatcc gctcgggcgc atcttcgacg
     3301 ctttcgggct ggagcgttgt ctgtggggca ccgactggac gcgcgcggtc gcgttactca
     3361 cttatgagca gggggttgaa gcttttcgag tcagcgaccg gctctcggac agcgatcggg
     3421 cgatgctcat ggggggcgcg ctggagcgcg tgtacaactg ggcgccgtcg cggctgaaag
     3481 cttagtcaat atggaagggc cggcgcacgc agtaccgtag attgcgcggg tggacgaagc
     3541 tcatcccgcg tagagctaca tgcgtggagg tcggcccagt gagaatgatg caacatcgac
     3601 ggccccggta catcggtctg gatgtgcacc gcgcgacgat cgccgtggcg atcgccgaag
     3661 aggagggttc gccgagcagc tacggcacga ttgccaacga tccgtctgcg gtgcgcaagc
     3721 tgatgacgcg cctgggtggc aaggacgtgg acttgcgggt tgcgtacgag gctggaccga
     3781 cgggttatgc attgcatcgg caactctcca agctgggcat ccaatgcatc gtggtggcgc
     3841 cctcgctgat accggtgcga cccggcgaca aggtgaaaac tg
//
LOCUS       DATGEM010000296         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1279312, whole genome shotgun sequence.
ACCESSION   DATGEM010000296 DATGEM010000000
VERSION     DATGEM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279312"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1328
                     /locus_tag="VKQ27_09485"
     CDS             51..1328
                     /locus_tag="VKQ27_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY89205.1"
                     /translation="MGFDAIARASRIRPGEPGLLRVALDTIINALGRRALEHERARLA
                     VRLEQARRLETVGTLASGIAHNFNNIIGAILGYVEIADEQHVAPRILGEIRKAGERAR
                     DLVDQILTFARRRDAQRSVFDLRTLATEAASLLRASLPAQVELAVHTAREPVSVLGVP
                     AQLQQVILNLCNNAAQAMDHAGRIDLDVALVDVPESAQFSHGVLGAGRYARITVSDTG
                     RGMDQAVLERLFEPFFTTRPTGNGLGLATARDTVREHGGAIHVLSAIGRGSRFEIWLP
                     YRPAGTRASGAALPFGEGEVVLVLECDAEQLLRDEEMLAALGYEPAGFTGMDAAWEAF
                     AESDQSFDLVLLAHSASMAEMLAFSERLHRLAPRLPILIATSAHDVFNAGQLLGAGIA
                     DVVPWPLAAVETALALQSALRRSQPAQAQYADS"
     gene            complement(1335..2405)
                     /gene="purM"
                     /locus_tag="VKQ27_09490"
     CDS             complement(1335..2405)
                     /gene="purM"
                     /locus_tag="VKQ27_09490"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HLY89206.1"
                     /translation="MTRGDQPQGLTYRAAGVDIEAGDALVETIKPLARATARAGVMGG
                     LGGFGALFDLKAAGFVDPVLVSGTDGVGTKLKIAIETGIHETVGIDLVAMCVNDIVVQ
                     GAEPLFFLDYFATGKLDVRQAREVIAGIASGCHEAGCALVGGETAEMPGLYRDGDYDL
                     AGFAVGAAERSALLPRGVAVGDALLGLASSGVHSNAFSLVRRVVEVSTLAWDAPAPFD
                     PETTLGAALMTPTRIYVPAVLALHRAGLLKAAAHITGGGLVGNVPRALPRGAAAVLEP
                     YWSVPAVFSWLAKAGCIAPDEMLRVFNCGIGMVLVVAAEDVERATEMLRDTGESVARI
                     GHIAAGDGEAQVRIEVPEGWPG"
     gene            2463..3029
                     /locus_tag="VKQ27_09495"
     CDS             2463..3029
                     /locus_tag="VKQ27_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLY89207.1"
                     /translation="MPDGSELDRSSTATLVTWPNVITFARLCAVPAAVWLVMEHWFAG
                     AFYLFAAAGLSDAVDGWLARRYGGNSVGAILDPVADKALLVSMYVTLAAVNVVPDWLA
                     ILVVFRDMVIVGGVVVLSLLGQSVMIRPLYVSKLNTVFQIVLIAVSLLLAGFDLVAPI
                     LVDALAWVVGATTFASGAAYVWTTVRGR"
     gene            3026..>3849
                     /locus_tag="VKQ27_09500"
     CDS             3026..>3849
                     /locus_tag="VKQ27_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLY89208.1"
                     /translation="MTGVADRPPPEEANPPPEPPPWFPEPPALAGRATRGQRVALGVG
                     LLVVLWLVLQLFASVLAPFVAAAGIAYVLDPPTTKLTHFGLPRGLAALVMILVLLAGV
                     LLFVLLLYPLILQQIGLLIGRIPQYVQLLQRWASEVISDLQQNFGSDVVNDKLRDLVS
                     SQAGSMLSVIASSLTSITNAGFAIFNVLTLAIVTPVVGFYLLRDWQRVIAMVDSWLPH
                     RYADVIRAQAREVDRILSAWVRGQALCCIFLALYYALALTIAGLDLGLIVGLAAGAL"
BASE COUNT          567 a         1493 c         1145 g          644 t
ORIGIN      
        1 ggggctggac gtgcgccacc gcaaccctcg ccgacggcac cgtggtgctg atgggcttcg
       61 atgccatcgc ccgtgccagc cgcattcgac ccggcgagcc cggcctgctg cgcgtcgcac
      121 tggataccat tatcaacgcg ctcggcaggc gcgcactcga gcatgagcgc gcccggctcg
      181 ccgtccggct ggaacaggcg cgccgcctgg aaaccgtcgg caccctggcc agcggcatcg
      241 cccacaactt caacaacatc atcggcgcca tcctcggcta tgtggaaatc gccgatgagc
      301 agcacgtggc accgcgtatt ctcggcgaga tccgcaaggc gggcgaacgg gcgcgcgacc
      361 tcgtcgatca gatcctcacc ttcgcccgcc gccgcgatgc ccagcgcagc gtcttcgacc
      421 ttcggacctt ggcaaccgag gcggcatcgc tgctgcgcgc atcgctgccg gcgcaggtcg
      481 agctggcggt tcataccgcc cgcgaacctg tctcagtgct cggggtgccg gcgcagttgc
      541 agcaggtgat cctcaacctc tgcaacaacg ccgcacaggc gatggaccac gctggtcgca
      601 tcgacctcga cgtcgcattg gtagacgtgc ccgaatcggc gcagttcagc cacggcgtgc
      661 tcggcgccgg ccgctacgcg cggatcacgg tgagcgacac cgggcgcggc atggatcagg
      721 cggtgctcga gcgcctgttc gagcctttct tcaccacccg ccccaccggc aacggcctcg
      781 gactggccac cgcccgcgac accgttcgcg agcacggcgg tgccatccac gtgctgagcg
      841 ccatcggacg cggcagccgc ttcgagatct ggctgccgta ccggcccgcc ggaacgcgcg
      901 catccggcgc cgcactgcca ttcggcgagg gcgaggtggt cctggtgctg gagtgcgacg
      961 ccgaacaatt gctgcgcgac gaggagatgc tggcggcact cggctacgag ccggcgggct
     1021 tcaccggcat ggacgcggcc tgggaggcgt tcgccgagtc agaccagagc ttcgacctcg
     1081 tcctgctggc gcattccgcc tcgatggcgg agatgctcgc attctccgag cggctccacc
     1141 gcctggctcc ccgcctgccg atcctcatcg ccacgtccgc ccatgatgta ttcaacgccg
     1201 gacaactgct tggtgccggc atcgccgatg tcgtgccctg gccgctcgcc gcggtcgaga
     1261 ccgcactggc attgcagagt gccctgcggc gcagccagcc cgctcaggcg caatacgctg
     1321 acagctgaag gccactaccc cggccagccc tccggcacct cgatccttac ctgcgcctcg
     1381 ccgtcgcctg ccgcgatgtg cccgatccgc gccacgctct cccccgtgtc gcgcagcatc
     1441 tcggtcgccc gctccacgtc ctcggcggca acgacaagca ccatcccgat cccgcagttg
     1501 aacacgcgca gcatctcgtc cggcgcgatg catcccgcct tcgccagcca cgaaaacacg
     1561 gccggcaccg accagtaagg ctccagcacc gcagccgccc cccgcggcag cgccctgggc
     1621 acattgccca ccagcccacc accggtgata tgagcggccg ccttcagcag ccccgcccgg
     1681 tgcagcgcca gcaccgccgg cacatagatc cgcgtcggcg tcatcagcgc cgcccccagc
     1741 gtggtctccg gatcgaacgg cgccggcgca tcccaggcga gcgtgctgac ctccaccacc
     1801 cgccgcacca gcgaaaacgc gttggaatga acgccagaac tcgccagccc cagcaaagca
     1861 tcccccaccg ccacgccccg aggcagcagt gcgctccgct ccgccgcccc gaccgcgaat
     1921 cccgccaggt cgtaatcccc gtcgcggtac agccccggca tctcggccgt ctcgccgccc
     1981 accagcgcgc acccggcctc gtggcagccc gacgcgatcc cggcgatcac ctcgcgcgcc
     2041 tgccgcacgt ccagcttccc ggtcgcgaag tagtccagga agaacagcgg ctccgccccc
     2101 tgcaccacga tgtcgttgac gcacatcgcc accaggtcga tgcccaccgt ctcgtggatg
     2161 cccgtctcga tcgcgatctt cagcttcgtg ccgacgccgt cggtccccga taccaacacc
     2221 gggtccacga accctgccgc cttcaggtcg aacagcgccc cgaacccgcc gagaccgccc
     2281 atcacccccg cacgcgccgt cgcccgcgcc agcggcttga tcgtctccac cagcgcatcg
     2341 cctgcctcga tatcgacgcc ggccgcgcga taggtcaaac cctgtggttg atcgcctctg
     2401 gtcatcatcg ccctcgatac gctactgcct tggaccgacg caatccgggg aaaagcaggc
     2461 ggatgccgga cggatcggag ctcgatcgca gcagcaccgc cacgctggtg acatggccca
     2521 acgtgatcac cttcgcgcgc ctctgcgccg ttcccgccgc ggtctggctg gtgatggaac
     2581 actggttcgc cggcgcgttc tacctgttcg ccgcggccgg actgtcggat gccgtggatg
     2641 gctggctcgc gcgccgctac ggtggcaact cagtgggagc aatccttgac ccggtggctg
     2701 acaaggccct cctggtgtcc atgtacgtaa cgctcgccgc ggtgaatgtc gtaccggact
     2761 ggctcgccat cctggtggtg ttccgcgaca tggtgatcgt gggcggcgtt gtggtgctgt
     2821 cactgcttgg ccaatccgtg atgatccgac cgctctacgt ctcgaaactt aataccgtgt
     2881 ttcagatcgt cctgatcgcc gtcagcctgt tgctcgccgg cttcgacctc gttgccccga
     2941 ttctggtgga cgcactcgcc tgggtcgtcg gcgccactac cttcgcctcc ggcgcggcct
     3001 acgtatggac cacggtgcgc ggccgatgac cggcgtcgcc gacagaccgc cgcccgaaga
     3061 agccaacccg ccgcccgagc ctcccccctg gttccctgag ccgcccgcgc tcgccggccg
     3121 tgccacccgc ggccagcgcg tcgccctcgg ggtcggcctg ctcgtcgtcc tctggctcgt
     3181 gctccagctc ttcgcctcgg tgctcgcgcc cttcgtcgcc gccgccggca tcgcctacgt
     3241 acttgatccg ccgactacga aactgaccca cttcggcctt ccccgaggcc tcgccgcctt
     3301 ggtgatgatc cttgtcctgc tggcgggcgt cctgctgttc gtcctgctgc tctacccgct
     3361 gatcctccag cagataggcc tgctcatcgg ccgcatcccg caatacgtgc agctgctgca
     3421 gcgctgggcg agcgaggtga tctccgacct ccagcagaac ttcggctccg acgtggtgaa
     3481 cgacaagctg cgcgaccttg tcagcagcca ggctggcagc atgctgagtg tcatcgcctc
     3541 ctcgctcacc agcatcacca acgcgggctt cgccatcttc aacgtgctga cgctcgccat
     3601 cgtcactccc gtcgtcggct tctacctgct gcgcgactgg cagagggtga tcgcgatggt
     3661 cgattcctgg ttgccccatc gctatgccga cgtgatccgc gcccaggcgc gcgaggtcga
     3721 tcgcatcctt tccgcctggg tccggggcca ggcgctctgc tgcattttcc tcgccctcta
     3781 ctacgcgctt gcacttacca ttgccggtct ggatctcggc ctcatcgttg gccttgcggc
     3841 cggcgcgct
//
LOCUS       DATGEM010000297         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_457809, whole genome shotgun sequence.
ACCESSION   DATGEM010000297 DATGEM010000000
VERSION     DATGEM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457809"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VKQ27_09505"
     CDS             complement(<1..791)
                     /locus_tag="VKQ27_09505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLY89209.1"
                     /translation="MRQWLMGALAAAVLVALPADAKIRTASIVVDSETGEVLSASNVD
                     SRIYPASLTKMMTLYLMFDDLESGKLHLSDRMPVSRKASSQRPSKLGLGAGQTLTVEN
                     ALYGMVVKSANDAAMCAGEYMGGGSEAAFAERMTRKAHELGMTRTTFKNPNGLPNPEQ
                     KSTARDLATLARALLHNHAKEYHYFSARQFNYNGATINGHNHLLDWYEGADGIKTGYI
                     DASGFNLVTSAKRDGRRLVGVVLGGASGPSRDRMMAKLLDAGFNRT"
     gene            1100..1822
                     /locus_tag="VKQ27_09510"
     CDS             1100..1822
                     /locus_tag="VKQ27_09510"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HLY89210.1"
                     /translation="MATAKTPKKVAAAAPAAAKSAARAVKAKPAPARAAVAKAPEDAI
                     VHSAEIVGEPAAQPARKAAAEVTKAVEKATEVAAKAVDATVAETSAEVTKTTDTVAKI
                     AEKPSKAVAVVGKAADKAQDSVLKGVVPFLDFSRDNAILLVAAGNELALGLHKLSLSL
                     LDWSAESCDKGVAAGQAMLSAKSVEEVFDLSQSLAKDGFNQLLKEGSELSALSSKLIE
                     DTIVPLPGRLVAAVEKLASHAA"
     gene            2039..2368
                     /gene="clpS"
                     /locus_tag="VKQ27_09515"
     CDS             2039..2368
                     /gene="clpS"
                     /locus_tag="VKQ27_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLY89211.1"
                     /translation="MSGDDNRNGDDGTQNGVVIKTRPKTKRPAMFKVLMLNDDYTPME
                     FVVHVLERFFSKNREEATRIMMHVHQRGVGVCGVFTYEVAETKVTQVMDLARQNQHPL
                     QCTIEKE"
     gene            2373..>3400
                     /locus_tag="VKQ27_09520"
     CDS             2373..>3400
                     /locus_tag="VKQ27_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY89212.1"
                     /translation="MLSRNLEASLHRALGLASERHHEYATLEHLLLSLTEDQEAISVL
                     RACNVDVDRLRTDLTEFVDSALADLVSARGGDPKPTAGFQRVVQRAAIHVQSSGREEV
                     TGANVVVALFSERESHAVYFLQLQDMTRLDAVNYISHGIAKTPGRSQSRPVHGADEDS
                     KGETTVKKGQEALNAYCVNLNKKAQAGKIDPLIGRDLEIERTIQILCRRSKNNPLYVG
                     DPGVGKTAIAEGLARRIVKNEVPDVLSNAVIYSLDMGALLAGTRYRGDFEERLKAVVS
                     ELEAIEGAVLFIDEIHTVIGAGATSGGSMDASNLLKPALATGALRCIGSTTYKEYRTH
                     FEKDRALVR"
BASE COUNT          654 a         1030 c         1093 g          623 t
ORIGIN      
        1 gtgcggttga agccggcatc cagaagcttc gccatcatgc ggtcgcgcga gggaccgctg
       61 gccccgccca ataccacgcc gaccagacgg cggccgtcgc gtttggccga ggtgaccaga
      121 ttgaagcccg aagcgtcgat atagccggtc ttgatgccgt cggcgccttc ataccagtcg
      181 agcagatggt tgtgcccgtt gatggtggcg ccgttgtaat tgaactggcg ggccgagaag
      241 tagtgatact ccttggcatg gttgtgcagc agcgcgcggg ccagggtggc gagatcgcgc
      301 gcggtgctct tctgctccgg attgggcagg ccgttcggat tcttgaaggt ggtgcgggtc
      361 atgccgagct cgtgcgcctt cctggtcatg cgctcggcga aagccgcctc ggaaccgccg
      421 cccatatatt cgccggcgca catcgccgca tcgttggcgg atttgaccac catgccatac
      481 agcgcgttct cgacggtcag ggtctggccg gcgccaaggc ccagcttgga cgggcgctgg
      541 ctggacgcct tgcgcgagac cggcatgcgg tccgacagat gcagcttgcc ggattcgaga
      601 tcgtcgaaca tcaggtacag cgtcatcatc ttggtgagag acgcgggata gatgcgcgaa
      661 tcgacgtttg aggccgaaag aacctcgccg gtttcggagt cgaccacgat ggacgcggtc
      721 ctgatcttgg cgtcggcggg caaggcgaca aggaccgcag ccgccagagc acccatcaac
      781 cactgccgca ttctgccatc ccctgagtgt gtcccgtgga atcaccataa aaaattcgtt
      841 accgcctgtc tatggcgcgc tgcaaccgat ttatggcaag ccctggcgga agtttctgcc
      901 agcaggggtt agaatgaggt taacgcggcg catggctgcc ctgtgacaat catgttgcgc
      961 tgcacaaaat tccttgacgt catggccggc ggaggatcat aagcgaggat tgctgcgccg
     1021 cgttcgaggg gctgaggcaa ctcgtcgtgt gcgcgatgtg ccccccaata agctcgttca
     1081 gtcagggagt tttcccacga tggccactgc caaaaccccg aagaaagttg cggccgccgc
     1141 tccggctgcg gcaaagtctg cggctcgtgc cgtgaaggcc aagccggcgc ccgcccgcgc
     1201 cgcggtggcc aaggcccccg aggacgcgat cgtccattcc gccgagatcg tcggcgagcc
     1261 ggcggctcag ccagcccgga aggctgccgc cgaagtgacc aaggccgtcg aaaaagccac
     1321 ggaagtggcg gccaaggctg tcgatgccac cgtggccgag acgagcgccg aagtgaccaa
     1381 gaccaccgac acggtggcca agatcgctga aaagccctcc aaggcggtgg ccgtggtcgg
     1441 caaggccgcc gacaaggctc aggacagcgt gctgaagggc gtcgtccctt tcctcgattt
     1501 cagccgcgac aatgcgatcc tgctggtcgc tgccggaaac gagctggcgc tgggcctgca
     1561 caagctgtcc ttgtcgctgc tcgactggtc ggccgagagc tgcgacaagg gtgtggccgc
     1621 gggccaggcc atgctgtcgg ccaagagcgt cgaggaggtt ttcgacctct cgcagtcgct
     1681 ggccaaggac gggttcaacc agcttttgaa ggaaggctcc gagctcagcg cgctgtcctc
     1741 aaagctgatc gaggacacca tcgttccgtt gcccggccgc ctggtcgcgg cggtcgagaa
     1801 gctggcttcc cacgccgcct gaatttgtca ccggaatatg tcgaccaggc tcacgaaggt
     1861 cactcttcgt gggccttatt tttgtgcttt atagctgcta tcgtgatggt gcccggttaa
     1921 gggattcgga tcgttgaaat gggtaggggg gcttttcccc ggcacttctc gaccaatata
     1981 tcggggaggg agattcccac cttaattccg acaccatcga cagttttgat tggcaggcat
     2041 gagcggagac gacaacagga acggcgacga cggcacgcag aacggcgtgg tcatcaagac
     2101 caggccgaag accaagcggc cggccatgtt caaggttctg atgctcaatg acgactacac
     2161 tccgatggag ttcgtcgtcc atgtgttgga gcggttcttc tccaagaatc gtgaagaggc
     2221 cacgcgaatc atgatgcatg tgcatcagag gggcgtcggc gtatgcggtg tttttaccta
     2281 cgaagtagct gaaaccaaag tgactcaggt gatggatctg gcgcgccaga atcagcatcc
     2341 cctgcaatgc acgatcgaga aggagtgagg ccatgctgtc gcgaaacctc gaggcgagtc
     2401 tccatcgggc attgggattg gcgtcggaac gccatcacga atatgccacc ctggaacatc
     2461 ttctgttgtc tctcaccgag gatcaggaag cgatctcggt attacgggcc tgcaatgtcg
     2521 acgtcgaccg gctgcggacc gacctgaccg aattcgtcga tagcgccctg gccgatctgg
     2581 tttcggcgcg cggcggcgat cccaagccga ccgccggttt ccagcgcgtc gtccagcgcg
     2641 ccgccatcca tgtgcaatcc tcgggacgtg aagaggtgac cggcgccaat gtcgtcgtcg
     2701 ccctgttctc cgagcgcgag agccatgccg tctatttcct gcagctgcag gacatgaccc
     2761 gcctcgatgc ggtgaactat atctcgcacg gcatcgccaa gacgccgggc cgcagccaga
     2821 gccgtccggt gcatggcgcc gacgaggatt ccaagggcga gaccaccgtc aagaagggcc
     2881 aggaggcgct caacgcctat tgcgtcaatc tcaacaagaa ggcccaggcc ggcaagatcg
     2941 acccgctgat cgggcgcgat ctcgagatcg agcgcaccat ccagatcctg tgccgccgct
     3001 cgaagaacaa tccgctctat gtcggtgatc cgggcgtcgg caagaccgcc atcgccgagg
     3061 gcctggcgcg gcgcatcgtc aagaacgaag tgcccgacgt cctgtccaac gcggtgatct
     3121 attcgctcga catgggcgca ctgctggccg gcacgcgtta ccggggtgat ttcgaggagc
     3181 ggctgaaggc cgtcgtctcg gaattggagg cgatcgaagg tgcggtgctg ttcatcgacg
     3241 agattcatac cgtgatcggc gccggcgcga cctccggcgg ctcgatggac gcgtccaacc
     3301 tgctgaagcc ggccctggcg acgggcgcgc tgcgctgcat cggctcgacc acctacaagg
     3361 aataccgcac ccatttcgag aaggaccgcg ctctggtgcg
//
LOCUS       DATGEM010000298         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_46824, whole genome shotgun sequence.
ACCESSION   DATGEM010000298 DATGEM010000000
VERSION     DATGEM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46824"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VKQ27_09525"
     CDS             <1..389
                     /locus_tag="VKQ27_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLY89213.1"
                     /translation="QIVEIAPKERLFSEPRHPYTRTLMTAIPRPDPHRHGTRQLPGGD
                     VPSPMNPPEGCRFHTRCPFVIDRCRAEMPLLRRWDDGHLTACHRAEELPPAGEVIEPG
                     HVAPMAAKRLALYAKRRAEMGTNAVG"
     gene            449..1636
                     /locus_tag="VKQ27_09530"
     CDS             449..1636
                     /locus_tag="VKQ27_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLY89214.1"
                     /translation="MTTVNANIVAPRLLLIAGGAVRQIADVLAKFELSRPLVVTDPFM
                     VSSGHVRRCLDPLAAAGIAVTIFSDTVPDPTDTVIEAGVAELKRGNFDCLIGFGGGSP
                     IDTAKAMAILAAGGGKMRDYKVPFAADKGALPIIAIPTTAGTGSECTRFTVITDVERD
                     EKMLIAGLGSLPLAAIVDYELTYTVPPRTTADTGVDSLTHALEAFVSKRANPLSDTLA
                     IAAMEMIGRHIRAAYAEPRNALAREGMMIGASTAGLAFSNASVALVHGMSRPIGAHFH
                     VPHGLSNAMLLPAVTRYSVPGAEGRYAEASRRIGFAVQQDADATAAAKLVAGLEALNK
                     ELSVPTPADYGISESVWNEKMELMAEQALASGSPANNPRVPNKGEIVALYREIWTGAP
                     RNA"
     gene            1711..3018
                     /locus_tag="VKQ27_09535"
     CDS             1711..3018
                     /locus_tag="VKQ27_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89215.1"
                     /translation="MADALEQRTMRKVYLHLLPLAMIVYFFCYLDRINISFAALQMNA
                     SIGLTAAAYGLSSTAFYLGYCIFEVPSNVILEKVGARIWIARIMITWGLASGATAFVT
                     GPTSFLTVRFLLGLAEAGLFPGIVLLFTYWFPDRHRARIVSSFTLALPISVALGAPIS
                     TSILGMDGFLGVAGWKWIYVMEAIPTILIGIFVLILMTDRPAKASWLSAEEKNWLTNT
                     LEHERRAVESARKFSMLEGMLNPKILLLSINYLGIVTASLGLLLFVPQIIKSLGATNM
                     GTGFATMLAYICGAISMLVWGWVSDRMGERRWNLFWACMLATLGLVLTGMTIGTWWAL
                     AGMCVATAGFYGTKGAFWAMPSMLLTGTAAAAGIAWINSIGNVGGAIGPAIVGWMKDV
                     TGSYAGGLYGLAIFTGVSALLAAFALHIPRQMPVRGGVGVAAE"
     gene            3228..4160
                     /locus_tag="VKQ27_09540"
     CDS             3228..4160
                     /locus_tag="VKQ27_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HLY89216.1"
                     /translation="MALAIWAAALASRWLLDTVVEAGPFLTFLPAIALTTVLCGRGPA
                     LVVIAASAVASDYFWLPPSGFSMEWPTTPMSLTLFILIGLFEMLLVDSLYRSSSGNAE
                     REGRLAMSLRLRETMFHELRHRIANQLHVIAATLEGAQIRIDQGAPPEDVLEQAIGRI
                     SSITYLQELLDDKTNYRRGVAPLLRDMLDHIFYDVDVAVQVRATPECLPEEQMTIICL
                     IVIEAAMNSLKHIFRRRRGHMFAVELRRLMDDRLLLTVWDDGPGFDAGTVMANPDGLG
                     LSIMQGLAAELGGTLSLETRGGTTVRVEFAEVAV"
     gene            complement(4164..>4837)
                     /locus_tag="VKQ27_09545"
     CDS             complement(4164..>4837)
                     /locus_tag="VKQ27_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY89217.1"
                     /translation="GQQLLAFDADAGIGAIVLTGNERAFAAGADIKEMKDREHPAAML
                     EDFIGARWETVLRVKKPVIAAVAGFALGGGCELAMMCDFILAADTAKFGQPEINLGVL
                     PGAGGTQRLTRAAGKSKAMDMILTARMMDAAEAERANLVSRVFSADTLIPEALKIAER
                     IAALSPVAVALAKQSVNRAFETTLAEGVRHERALFLSLFGTHDQREGMAAFAEKRKPA
                     FKLGG"
BASE COUNT          825 a         1471 c         1561 g          980 t
ORIGIN      
        1 gccagatcgt tgagatcgct ccgaaggagc gattgttctc cgagccgcgg catccgtata
       61 cgcgcacatt gatgacagcc attccacggc cggacccgca ccgccatggg acacgccagt
      121 tgccaggtgg cgacgtgccg agcccgatga acccgccgga aggctgccgg ttccacaccc
      181 gctgcccgtt cgtgatcgat cgttgccgcg cggaaatgcc gttgttgcgc cggtgggatg
      241 acggccatct cactgcctgt catcgcgcgg aggagttgcc gcccgcgggc gaagtcatcg
      301 agccgggtca cgtagctccg atggcggcga aacgcctggc actctacgcg aaacggcgag
      361 cagagatggg aaccaatgcc gttggatgat gcgccgtatt tgggtgcata atccataaag
      421 cgaccctcgt ccaagggagg agcgtgccat gacgacagtg aatgccaata tcgttgcccc
      481 ccgcctactg ctcattgctg gtggtgccgt gcgccagatt gccgatgtgc tggcgaagtt
      541 cgaactgtcg cggccgctgg tggtgactga tccgttcatg gtgtcgtctg gccatgtgcg
      601 gcgctgcctg gatccgctcg ctgccgctgg tatcgctgtc accatattca gtgacacggt
      661 gcctgatccc accgatacag tgatcgaggc aggcgtcgcc gaactgaagc gcggcaactt
      721 cgattgcctt ataggctttg gtggcggctc gccgatcgac accgcgaagg cgatggccat
      781 cctggcggct ggcggcggca agatgcgcga ttacaaagtg ccgttcgcgg ccgacaaagg
      841 cgcactgccg atcattgcca ttccgaccac cgcgggcacg ggcagcgaat gcacgcgctt
      901 cacggtcatc accgatgtgg agcgcgacga gaagatgctg attgccggcc ttggctcgct
      961 gccccttgcg gctatcgtgg actatgagct tacctatacc gtcccgccgc gcactaccgc
     1021 cgataccggc gtggacagtc tcacgcatgc gctggaggca ttcgtgtcga agcgggcgaa
     1081 cccgctatcc gatacgcttg cgattgccgc gatggaaatg atcggccgcc acatccgcgc
     1141 cgcttacgcg gagccgcgca acgcattggc gcgcgagggc atgatgatcg gtgccagcac
     1201 cgcgggcctg gcattctcca atgcctcggt cgcgctggtg cacggtatgt cgcggccgat
     1261 cggcgcgcat tttcacgtgc cgcacgggtt gtccaacgcc atgctgcttc ccgcggtcac
     1321 gcggtattcc gttccggggg cggaaggacg ctatgccgag gcgtcgcgac gtatcggctt
     1381 tgctgtccaa caggatgccg atgcaacggc tgctgccaag ctggttgccg ggctcgaagc
     1441 gttgaacaag gaactgtcgg tgccgacgcc ggctgattac ggcatcagcg agagcgtgtg
     1501 gaatgagaaa atggaactga tggcagagca ggcacttgca tcgggcagcc cggccaacaa
     1561 cccacgcgtg ccgaacaagg gcgaaatcgt agcgctgtac cgggaaatct ggactggcgc
     1621 gcctcgcaac gcctgaacag ggccgttgcc attcgtcgtg ccgccttgct agggttcgtt
     1681 tcaagtaaga cgaaccctag ggagtaaatg atggccgacg ctcttgaaca gagaacgatg
     1741 cgcaaggtgt atttacacct gttgccgctc gcgatgattg tctacttctt ctgctatctc
     1801 gatcgcatca atatcagctt tgccgcattg cagatgaacg cgagcatcgg cctgaccgct
     1861 gccgcatacg gcctgagctc cactgcgttc tatcttggct attgcatctt cgaagttccg
     1921 agcaacgtca tcctggaaaa ggtcggtgca cgcatctgga tcgcgcgcat catgatcacc
     1981 tgggggctgg cgtccggcgc gacagcgttc gttacggggc cgaccagctt cctgaccgtg
     2041 cgctttctgc ttgggctggc cgaagccggg ctcttcccag gcatcgtgct gttgttcacc
     2101 tattggtttc ccgatcgcca ccgcgcgcgc attgtctcca gcttcacgct ggcgttgcca
     2161 atctcggtcg cgttaggcgc accgatctcc acatcgatcc tcgggatgga cggtttcctg
     2221 ggcgttgccg gctggaagtg gatctacgtc atggaggcaa tcccgacgat tttgatcggt
     2281 atcttcgtgc tgatcctgat gaccgatcgg ccagcgaagg caagctggct gtccgccgag
     2341 gaaaagaact ggctgaccaa cacgctggaa catgagcggc gcgccgtcga atcagcgcgc
     2401 aagttcagca tgctggaagg catgctgaac ccgaagatcc tgttgctgtc gatcaactat
     2461 cttgggatcg tgaccgccag cctcggtttg ctgctgttcg tgccgcagat catcaagtcg
     2521 ctcggcgcga cgaacatggg taccggtttc gcgaccatgc tcgcgtatat ctgcggcgcg
     2581 atcagcatgc tggtctgggg ctgggtttcc gaccgcatgg gcgagcggcg ctggaatctg
     2641 ttctgggcct gcatgctggc tacccttggg ctggtgctga ccggcatgac catcggcacc
     2701 tggtgggcgc ttgccgggat gtgcgtcgca accgccggtt tctacgggac caagggagcc
     2761 ttctgggcca tgccgtccat gctgctgacc ggcacggccg cagccgcggg catcgcctgg
     2821 atcaactcca tcggcaatgt cggcggagcg atcgggccgg cgatcgtcgg ttggatgaag
     2881 gatgttaccg gcagctacgc tggcgggctg tatggcctgg ccatatttac cggcgtctct
     2941 gcgctgcttg cggcctttgc tctgcacatt ccccggcaga tgccggtgcg tggcggcgta
     3001 ggcgtcgcgg ccgagtagca ctgacgtcag cgggccggac actcggcgcc ggcccgctgg
     3061 ctacgcgacg tttcattcgt taggaacgtc ggctgaatga gtctatacac gtggcaatgc
     3121 taggttcacc gcacatggag gcatggacgc gggaggcgcc aaatgtgggg gcgtccgtag
     3181 tcgtgccgcg gctgcgccgg catcggagcg tttgggttcg ttggctgttg gcgttagcca
     3241 tttgggcggc cgcgcttgcc tcgcggtggt tattggacac tgtggtcgag gccggccctt
     3301 tcctgacgtt cctgcccgcg atcgccctga cgacggtgct ctgcggacga gggccggcgc
     3361 tggtggtcat cgctgcctcg gccgtggcca gtgattactt ctggttgccg ccgtctggct
     3421 tttcgatgga atggccaacc acgccaatgt ccctgaccct gttcatcctg atcggactat
     3481 tcgaaatgct gctggtggac tcgctctatc ggtcctcgag cggcaatgcc gagcgagagg
     3541 ggcggctggc aatgtcgttg cgcctgcgcg aaacgatgtt ccacgaattg cgccaccgta
     3601 ttgccaatca gctgcatgtc atcgccgcta cgctggaggg agcgcagatc cggatcgacc
     3661 aaggtgcgcc accggaagat gtgcttgagc aggcgatcgg acgcatttcc tcgatcacct
     3721 atttgcagga gctgctggac gacaagacga attatcgccg cggcgtggca ccattgctgc
     3781 gcgatatgct ggatcacatc ttctatgacg tggatgtcgc cgtacaggtg agagcgacac
     3841 ccgagtgcct gccggaggag cagatgacga tcatctgcct gatcgtgatc gaggcggcga
     3901 tgaactcatt gaagcacatc tttcgccggc gccgcggcca tatgttcgca gttgaactcc
     3961 ggcgactgat ggatgatcgg ctcttgctga cagtgtggga tgacgggccg ggtttcgatg
     4021 ccggcacggt catggccaac cccgacgggc tcggcctttc catcatgcaa gggctggccg
     4081 ccgagcttgg cggcacgctc agcctggaga cccgtggcgg caccactgtg agggtggagt
     4141 tcgccgaagt cgccgtgtag ctgctatccg ccgagcttga acgccggttt ccgcttttcg
     4201 gcgaaagctg ccatgccttc gcgctgatcg tgcgtgccga acagcgacag gaacagcgca
     4261 cgctcgtgcc ggacgccttc ggccagcgtg gtttcgaacg cgcggttcac tgactgcttc
     4321 gcgagggcaa cggccaccgg tgaaagtgcg gcgatgcgtt cggcgatctt cagtgcctct
     4381 ggaatgagcg tatcggcaga gaacacacgg gatacgaggt tggcgcgctc tgcctctgct
     4441 gcatccatca tgcgagcggt caggatcatg tccatcgcct tggattttcc cgccgcgcgc
     4501 gtcagccgct gtgtgccgcc cgcgccaggc agaacgccga ggttgatctc aggctggccg
     4561 aacttcgcgg tgtcggcggc gaggatgaag tcgcacatca tcgccagttc acagccaccg
     4621 ccgagcgcga agccggccac cgcggcgatc accggcttct tcacgcgcag cacggtttcc
     4681 cagcgcgcgc cgatgaaatc ctcgagcatt gccgcggggt gttcgcggtc cttcatttcc
     4741 ttgatgtcgg cgcctgcggc gaaggcccgc tcgttgccgg tcagcacgat ggcgccgata
     4801 cccgcatccg catcgaaggc gagaagctgc tgtccga
//
LOCUS       DATGEM010000299         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_884095, whole genome shotgun sequence.
ACCESSION   DATGEM010000299 DATGEM010000000
VERSION     DATGEM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884095"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..837
                     /locus_tag="VKQ27_09550"
     CDS             61..837
                     /locus_tag="VKQ27_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89218.1"
                     /translation="MRWSVSGIIISLLLALPVPSRAGSEPVIVCNAPDEFLTTDAPLA
                     EFGAAIAAGGPVNILAVGSATTVGAPSTKDQKPTAIEGGTFPVQMVRSLNAAMPSVRF
                     TLTVRGGRGMTADAMLKLLDETLKQQHYPLVLWQTGTVEAVRGLRPDGLLDVLHQGAD
                     AVSDGGGNLVLIDPQFSRFLRANTDVDAYEDVMRQAAANPGVALFQRFDLMRSWADDG
                     SVDLERTAKEDRDKALDRLNSCLGQVLAKFVLSGADAAKK"
     gene            904..1818
                     /locus_tag="VKQ27_09555"
     CDS             904..1818
                     /locus_tag="VKQ27_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89219.1"
                     /translation="MAGMLEGKVAVVTGAGRGIGRATAICLAANGAKVVVCDVGAAVT
                     GEGHDVGPAQQVVSEIDQAHGKGRAVANTDSVAEWENAQKIVQTAIDNFGRIDMVVNN
                     AGILRDRMFHYMSPEEWDAVIKVHLYGCFYVSRAAVPHFRKQESGCFVHITSTSGLIG
                     SVGQTNYGAAKLGIAGLSRCIAMDMQRYKIRSNCIGPHAFSRMIETVPGQSEEQLKAR
                     AERTRPDHIAQLIAFLGTDAAEGVSGQIFGVRGNEVYLYNQPRPIRIVARTDGWTPEK
                     LAEHWLPAVKNSFTPLERTRDVYPWDPV"
     gene            complement(2205..3698)
                     /locus_tag="VKQ27_09560"
     CDS             complement(2205..3698)
                     /locus_tag="VKQ27_09560"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HLY89220.1"
                     /translation="MSTLLNDRPLELEIKFRLHGGARAALAEHPALSGNHRAQREVST
                     YFDTADGDLRRAGATLRVRRHGARRVQTVKLQGPGGAFARGEWEWPVQTDTPDLRRLA
                     ETPIAGMANSPLEPIFVTDVQRDVREVRQDGATIEVAIDRGHVRAGNAAEEIAELELE
                     LKQGDPGALYRLAIGLHADATLSLAAESKADRGWRLRTGQPRPAERHTDVDLPAGITA
                     TEAFRRILDNTLGHFMANHPAATSGSIEGVHQMRIAIRRGRAALLLFKPQLEPHAAAR
                     FTDALRQLGRVFGEARDWDVFCTDMLNSAESGGVARSWLDLLRQPAETQRIAAHTRVA
                     TELEAPVLTSTVLGLQAWDIASDTSLVDVAPDLVASLDQKVRHRGRRIAKLDYEGLHK
                     LRKSLKKLRYGIEFLAPLLKEEDTKRYLHRCKALLKHLGALNDAVVAVKLAEQLGGER
                     QPELAPAVSALAEWTDDRRSKIRRDIEDAWAKLQDAQLPACSHGAKR"
     gene            complement(3809..>4634)
                     /locus_tag="VKQ27_09565"
     CDS             complement(3809..>4634)
                     /locus_tag="VKQ27_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLY89221.1"
                     /translation="MASTRLDGPKERGGTQAMSVDLIIEGPVAKIVLNRPEKLNALTY
                     PMRAQLRDYTQQLRFDDNVRAIIVTGEGRAFCTGADVSGMQRGGLRAERQKMQGGSHQ
                     FLRNLHAIEKPVIAAVRGPTVGIGWSIALACDLIVASETALFSQVFRRIGLAPDGGAI
                     WFLTRRIGLSRAKELVFSARFVEAPEALELGLVNHVVPDGELMAKTEELAADLATGPT
                     FAFGMAKKLFHMASGPSYDDFLDMEAFVQPQLDQTDDHREGVAAFKEKRKPNFVGR"
BASE COUNT          780 a         1379 c         1591 g          884 t
ORIGIN      
        1 gttccgcgcc aaacagtcag cttgccggcg gtcgcgcgga ctgataccgg ttaggcgtat
       61 atgcgctggt cggtctcggg aatcataatc agcctgttgc ttgcgttgcc ggtaccgtcg
      121 cgggccggca gcgaaccggt catcgtctgc aacgcgccgg atgaattcct gacaaccgac
      181 gcgcctctgg cagagttcgg cgcggcgatc gccgcgggcg ggccggtcaa cattctggca
      241 gtcggttctg cgaccactgt cggcgcgccg agcaccaagg accagaagcc caccgccatc
      301 gagggtggta ccttccccgt gcagatggtc cgctcgctga atgccgcgat gccgtccgtg
      361 cggttcacgc tgaccgtgcg gggcgggcgt ggcatgacgg ccgatgcgat gctgaagctg
      421 ctcgatgaga ctctgaagca gcagcattat ccgctggtgt tatggcagac cggcacggtg
      481 gaggcggtgc gtggcctgcg tcctgacggt ctgctggacg tgctgcacca gggtgccgac
      541 gcggtgagcg atgggggcgg caatctggtg ctgatcgatc cgcagttcag ccgcttcctg
      601 cgggccaaca ccgatgtcga cgcctatgag gacgtgatgc gtcaggcggc ggcaaatccc
      661 ggtgtcgcgc tgttccagcg ctttgatctg atgcgttcgt gggcggatga cggcagcgtc
      721 gatctggagc gcacggcgaa ggaggaccgc gacaaggcgc tcgatcgatt gaactcctgt
      781 ctcggccagg tgctggcgaa gttcgtgctg agcggcgccg acgcggcgaa gaagtagggt
      841 gggatgacac acggcccact cgggcgcatt ctttgcccat cctgttcaac ggaggaaacg
      901 tgaatggctg ggatgcttga gggcaaggtt gccgtggtca ccggcgcggg gcgcggaatt
      961 gggcgtgcga ccgcgatctg cctggcggcg aatggcgcga aggtcgtggt gtgcgacgtg
     1021 ggcgcggcgg tgaccggtga aggccatgac gttggaccgg cgcagcaggt ggtgtctgaa
     1081 attgatcagg cgcatggcaa gggccgcgcc gtggcgaaca ccgacagcgt cgcggagtgg
     1141 gagaatgccc agaagatcgt ccagacggcg atcgacaatt tcggccgcat cgacatggtg
     1201 gtgaacaacg ccggcatcct gcgtgaccgc atgttccact acatgtcgcc tgaagagtgg
     1261 gatgcggtca tcaaggtgca cctttatggc tgcttctatg tcagtcgcgc agcggtgccg
     1321 catttccgca agcaggaaag cggctgcttc gtgcacatca cctccacatc ggggctgatc
     1381 gggtcggtgg ggcagacgaa ctatggcgcg gcgaagctgg gcatcgcggg attgtcgcgt
     1441 tgtatcgcga tggacatgca gcgctacaag atccgctcca actgcatcgg gccacatgcg
     1501 ttcagccgca tgatcgagac agtgccgggg cagagcgagg agcagttgaa ggcgcgtgcc
     1561 gagcggacgc ggccggatca tatcgcgcag ttgatcgcgt tccttggtac tgatgccgcg
     1621 gagggtgtgt cggggcagat ctttggcgtg cggggcaacg aggtctatct ctacaaccag
     1681 ccgcggccga tccgcatcgt ggcgcggacg gatgggtgga caccggagaa actagcggag
     1741 cattggctgc cggcggtgaa gaacagcttc accccgctgg agcggacacg cgacgtctat
     1801 ccgtgggatc cggtgtaagc gggcgactat ccgggcggag cgggggcggt ctccaggagg
     1861 gatcgcttcg cttcgctgtc tagcgctgtg ttcgaggaat tggggcttgc ggtagtctgg
     1921 tgctggggga cattccggcg ataactgcta ctggggcaat agttaagcgg attggctcgc
     1981 ggtgcgcgcc actgcacgga tttcacggaa agtatttatt tattactact tggcgttgca
     2041 gcatatagta aatatcaatc tatacagtgt aatctgtgca gtggcgcgca tcgcgagcca
     2101 atctgcgcaa tccgtgttaa ggcttgccct gccgccaacg gcacggaagc cgcgcaaagg
     2161 tgggcgccat tcacgcagtc gccaccactc aacgaagagg gacgtcagcg cttcgcgccg
     2221 tgggagcaag cagggagttg cgcatcctgc agcttggccc acgcgtcctc gatgtcccgg
     2281 cggatcttgc ttcgccggtc gtccgtccac tcggcgagcg ctgataccgc aggcgccaat
     2341 tccggctgac gctcgccacc gagctgttcg gccagcttca ccgcgacgac cgcatcgttc
     2401 aacgccccga ggtgtttcag cagcgccttg catcgatgca gatagcgctt ggtgtcctct
     2461 tctttgagaa gcggcgccag gaattctatg ccatagcgca gcttcttgag tgacttgcgc
     2521 aacttgtgca ggccctcgta atcgagcttc gcgatgcggc ggccgcgatg gcgcaccttc
     2581 tggtcgagac tcgccaccag gtctggcgct acgtcaacga gtgaggtgtc gctggcgata
     2641 tcccacgcct gcagaccgag tactgtcgag gtcagcaccg gtgcctcgag ctcggtggcg
     2701 acgcgtgtat gtgccgcgat acgctgtgtt tctgccggtt ggcgcagcag atcgagccat
     2761 gacctggcga cgccacctga ctcggcactg ttgagcatgt cggtgcagaa cacgtcccaa
     2821 tcgcgggctt cgccgaatac gcgccctagc tggcgcagag catccgtgaa acgggccgcg
     2881 gcgtgcggct cgagttgcgg cttgaacagc aatagcgcgg cacgaccacg gcggatggca
     2941 atgcgcatct gatgcacacc ctcgatgcta cccgacgtgg ccgctggatg gttggccatg
     3001 aaatggccga gcgtgttgtc gaggatgcgg cggaatgcct cggttgcagt gatgccggcc
     3061 ggcaggtcga catccgtgtg tctttccgcc ggacgcggct gaccggtgcg taggcgccag
     3121 ccacgatccg ccttgctttc ggcggcgagg cttagcgttg catcagcgtg caggccgatt
     3181 gccaatcgat acagggcgcc ggggtcgcct tgcttcagtt cgagctcgag ttcggcaatt
     3241 tcctccgcgg cgttaccggc tcggacgtgg ccccgatcga tcgccacctc gatcgtcgcg
     3301 ccatcctgac ggacctcgcg gacatcccgc tgcacgtcag tgacgaagat cggctccaag
     3361 ggcgagtttg ccatgccggc tataggtgtt tctgcgaggc gccggaggtc tggcgtatcg
     3421 gtctgaacag gccattccca ctcaccacgc gcgaatgcgc caccgggacc ctgaagcttc
     3481 accgtctgca cgcgtcgcgc gccgtggcgg cgcacgcgca acgttgcgcc ggcgcgtctc
     3541 aggtcgccgt cagcggtatc gaaataggtg gagacctcgc gttgcgctcg atgattgccg
     3601 gacagcgcgg ggtgctcggc cagggcggcg cgcgcgcctc catgcagccg gaacttgatc
     3661 tcgagttcca gtggccgatc gttaagaagg gtgctcatga attcctgcaa cgcgcggcgc
     3721 gccatcgcgt tgccccccga atgtcgtcta gcgccggcac tcttcgtcgt gcgcggcggg
     3781 cgtggcattg acctggatca aaggtgggtc aacggccgac gaagttcggc ttccgctttt
     3841 ccttgaaggc ggcgacacct tcgcggtggt cgtcggtctg gtcgagctgc ggttggacga
     3901 aggcttccat gtcgaggaag tcgtcgtagc tggggccgct cgccatgtgg aacagtttct
     3961 tcgccatgcc gaatgcgaag gttggacccg tcgccaggtc ggctgccagc tcttcggtct
     4021 ttgccatcag ttcgccgtcc gggacgacgt ggttcaccaa tccgagttcc agtgcttccg
     4081 gggcttcgac gaagcgggcg gagaagacga gctccttggc gcgagacagg ccgatgcggc
     4141 gggtcaggaa ccagattgcg ccgccatccg gcgccaggcc gatacggcgg aagacttgtg
     4201 agaagagcgc agtctcggat gcaacgatca ggtcgcaggc gagcgcgatg ctccagccga
     4261 tgccgacggt ggggccgcga acggcggcga tcaccggctt ctcgatcgca tgcaggttgc
     4321 gcaggaactg gtggctgccg ccttgcatct tctggcgctc ggcgcgcagg ccgccacgct
     4381 gcatgccgct gacgtcggcg ccggtgcaga aggcgcggcc ctcgccggtg acgatgatgg
     4441 cgcggacgtt gtcatcgaag cgaagctgct gcgtatagtc acggagttgc gcgcgcatcg
     4501 ggtaggtgag cgcgttcagt ttctccggcc ggttcagtac gatcttcgcc accggcccct
     4561 cgatgatcag gtcaaccgac atcgcctgcg ttcctccgcg ttccttcggt ccatccaagc
     4621 gtgttgacgc catg
//
LOCUS       DATGEM010000300         8254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_377086, whole genome shotgun sequence.
ACCESSION   DATGEM010000300 DATGEM010000000
VERSION     DATGEM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377086"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..892
                     /locus_tag="VKQ27_09570"
     CDS             191..892
                     /locus_tag="VKQ27_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLY89222.1"
                     /translation="MQAVVDALGMTPLFERLELPLRQRIEAISETVEVGAEHVLSQQG
                     ALPMHLYVLLEGQVALSSTAADGTTALVEVLHPLDHFVLASVLSRLPNLMTARTVGPA
                     RVVAIDADGLMDIVEHEAAVANALLRSVSREFRTMVRQVRDLKLRTAAQRLGCYLLAR
                     VQDGTAMQAEFRLPFDKGLLAARLGCRQENLSRAFAALRGYGVETHGSRVILHDIPRL
                     KALAVPDEVSDPGAF"
     gene            965..1270
                     /gene="rpmB"
                     /locus_tag="VKQ27_09575"
     CDS             965..1270
                     /gene="rpmB"
                     /locus_tag="VKQ27_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLY89223.1"
                     /translation="MSRRCQITGKGVLAGNNVSHANNRTRRRFLPNLQDASLLSDILG
                     LPVRMKLSTRAIRTIEHNGGIDAYILGTREGLLSPDAKALKRRLLRAQAKRAASAAA"
     gene            1294..2115
                     /gene="prmC"
                     /locus_tag="VKQ27_09580"
     CDS             1294..2115
                     /gene="prmC"
                     /locus_tag="VKQ27_09580"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HLY89224.1"
                     /translation="MDDAAARLRVAGIDNPRLEARLLLAHALGLAPADVLRDRDTLVD
                     GDALLPLVIRRAAREPLALILGRREFWSLDFAVSPATLIPRPESETLIEAALAAFAGR
                     DPPDRVLDLGTGTGCLLLAALSEFRSAFGVGVDRSAEAAPLAKANASALGLAGRAAFV
                     CADWAGALGARFDLILCNPPYIPTSCLPGLMPDVAEHEPWSALDGGSDGYAAYRHLLP
                     EILRLLQPDGVAVLELGLDQADAVGDMARRMGLVVDFRNDLSGIERAVLLRRTSP"
     gene            2383..2847
                     /locus_tag="VKQ27_09585"
     CDS             2383..2847
                     /locus_tag="VKQ27_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HLY89225.1"
                     /translation="MNMKRMRGRGHRPGGGGGGGGLRHHGGGSGSGIPLNRNHVFDSS
                     GPDLRIRGTAQQLFEKYLQLGRDATSGGDRVMAEGYFQHAEHYFRILNAMNQAAQQSQ
                     QQNGGQRRPYNGEEGGQPSDQPPEEAEVRSPAGVGEQPEAREIPIAAAPPEA"
     gene            2933..3715
                     /locus_tag="VKQ27_09590"
     CDS             2933..3715
                     /locus_tag="VKQ27_09590"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLY89226.1"
                     /translation="MRWARFEQNGSPAYGVVEGDEIIPVRGSPFEMWERTAARLPIAS
                     VKLLVPVVPLTFYAAGLNYAEHVREAAEKLGLKVELPTAADAGYRANNALIAQGEAIV
                     IPEDATEQVQYEGELVAVIGRKCKHLARENALSAVLGYTIGNDVSERTWQKSDRTLWR
                     AKNTDTFKPMGPWIETNVKLDELVTRVRLNGEERSVFPTNHMVFGVVDFLVAMTKYLT
                     LYPGDVLWMGTEGATANMKHGDVCEVEISGIGTLRNPVVRAS"
     gene            3987..5168
                     /locus_tag="VKQ27_09595"
     CDS             3987..5168
                     /locus_tag="VKQ27_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HLY89227.1"
                     /translation="MQRDVVVVSGVRTAIGDFGGGLKDVSPTDLGAQVVREALSRASV
                     DGAEVGQVVFGNVIHTEPKDMYLARVAAMNAGVAKEAPALTLNRLCGSGLQAVVSAAQ
                     TIMLGDSEIAVAGGAESMSRAPYLAQGMRWGARMGDAAMIDMMLAALHDPFDNIHMGE
                     TAENLAESHQITRGDQDALALESHRRASRAIAEGRFKQQILPIKLKTRRGETIVDTDE
                     HPRADQTEDALAKLRPVFRKDGTVTAGNASGINDGAAAVVLMERATAAKRGVKPMGRL
                     VAYAHAGVDPKIMGIGPVPATRLALQRAGLAVGDIDVIESNEAFAAQACAVAKDLGFD
                     PAKTNPNGSGISLGHPVGATGCILTVKALYELERIGGRYALVTMCIGGGQGIAAVFER
                     L"
     gene            5197..5721
                     /locus_tag="VKQ27_09600"
     CDS             5197..5721
                     /locus_tag="VKQ27_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89228.1"
                     /translation="MAGGQRGSSAVCRCGETAIELSGEPIQSVICFCESCRTAARGFE
                     RDLGAPPTVNADGGVHYCLYRKDRVKIARGAHHLREFRLKPDSPTRRVVASCCSSPMF
                     VEFTRGHWLTMFLDRLSGQAPEPQMCVMTKYRPEGLKLPRGIPAYGTLPPSLMIKLLA
                     AWAAMGFRRPTIVW"
     gene            complement(5931..7055)
                     /locus_tag="VKQ27_09605"
     CDS             complement(5931..7055)
                     /locus_tag="VKQ27_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HLY89229.1"
                     /translation="MMPFIDLKAQQARIAVELRRRLDGVLAHCQFILGPEVAELEREL
                     AAFCGARHCVTVSSGTDALQIALMAENVGRGDAVFLPAFTYTATAEVPLVLGATPVFV
                     DVDPHTFQIDPAHLEQRITEIRRAGKLRPRALIGVDLFGQPADWPALAAIARREDLFT
                     VDDLAQSFGCTQQGRPLGAAADATATSFFPSKPLGAYGDGGALFTESDDRAALYRSLR
                     THGEGMSRYEVLHTGMNGRLDSMQAAVLLAKMTVFPNELAIRERIARYYDSRLGNAVT
                     TPARVPDSTSAWAIYAILLRDAATRDHVQASLRANGVPTAIYYPRPLHTQPAYQDHHD
                     GSHLPVSEDLAERILALPIHPDMSEENMAHVCDQVLAAVG"
     gene            complement(7052..>8254)
                     /locus_tag="VKQ27_09610"
     CDS             complement(7052..>8254)
                     /locus_tag="VKQ27_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HLY89230.1"
                     /translation="LQIRRAPRPTALDTATERGDKLELRPMAIEDLLNRAQVPLDREG
                     MARLIQGRRVIVTGAGGTIGSELARQVAALGPERLILVDNGEYALWLIDLELAERHPH
                     VPRESLLADIRDEGRIRAVFEQWRPELVFHAAALKHVPMVEANPLEGLLTNVAGTRHV
                     ADAARAVGSLAMVLISTDKAVNPTSVMGASKRLAEMYCQGLDIASHGMRCVTVRFGNV
                     LGSTGSVVPLFQRQLERGGPLTVTHPDMQRYFMTVREAVGLVLQASVVRVGNADLPAR
                     DEGGIFVLDMGPPVKIVDLARQMVRLAGLVPDRDVEIRFTGLRPGEKLHEELFHGTEP
                     PVPTNYPGLLMAAPRTADPAIVGRAIDEIAGACRGGQSRLALTLLGRLVPEFEHNPEG
                     LMPVVNRP"
BASE COUNT         1373 a         2370 c         3039 g         1472 t
ORIGIN      
        1 cattttaagt gccttatgtc tgccaattcc atcacgcccg cttgatggca agcctagcac
       61 caacgaccga tccggacacg ccgagagccg tgcgtgccag aggcaatcta gaccgcgttc
      121 agcagccgag attattttaa caaatcgtgc ggggaatggg ataataagcc gggcgccggg
      181 tggggctgat atgcaggcag tcgttgatgc gttgggaatg accccactgt tcgagcggct
      241 agagcttccg ttgaggcaac ggatcgaggc aatcagtgaa accgtcgaag ttggcgccga
      301 gcatgtgctc tcccagcagg gcgcgctgcc gatgcacctt tatgtgctat tggaggggca
      361 ggtcgccctg agcagtactg ccgccgatgg cacgacggca ttagtcgagg tgctgcatcc
      421 gctcgaccat ttcgttctgg cctccgtcct gtcgcggctg cccaatctga tgacggcccg
      481 cacggtagga cctgcccgtg tggtggcgat cgacgcggac ggactgatgg acatcgtgga
      541 gcacgaggcg gcagtcgcca acgccttgct gcgttcggtc tctcgcgagt tccggaccat
      601 ggtgcgtcag gtccgcgatc tcaaactgcg gaccgccgca cagcggctgg gctgttatct
      661 gttggcgcgg gtgcaggatg gcacggcgat gcaggcggaa tttcgcctgc cattcgacaa
      721 ggggctgctt gcggcccgtc tcgggtgccg ccaggagaac ctgtcgcgcg ccttcgccgc
      781 gttgcgcggc tacggcgtcg aaacccatgg ctcccgcgtc atcctgcacg acattccgcg
      841 cctgaaggct ctggcggtgc cggacgaggt cagcgatcct ggtgcattct gacctctggc
      901 cttgacgctg ccgggacgat tggcgataag ccgcgccttc cgatcggacc aaggtgcaat
      961 cacgatgtct cgccgctgtc agatcaccgg caagggcgtg ctggccggta acaacgtcag
     1021 ccacgccaat aacaggaccc gccggcgctt cctgcccaat ctccaggacg cgtcgctgtt
     1081 gtccgacatc ctgggcctgc cggtcaggat gaagctttcc acccgcgcca tccgtaccat
     1141 cgagcacaat ggcggcatcg atgcgtatat cctggggacg cgggaggggc tgctgtcgcc
     1201 tgacgcgaag gccctgaagc gccggctgct gcgcgcgcag gcgaagcggg cggccagcgc
     1261 cgcggcctag ccgcgaccac gatccgacaa gctattgacg atgctgccgc gcggttgcgc
     1321 gtggctggca ttgacaatcc gcggttggag gcccggttgc tgctcgcgca cgcgctcggt
     1381 ctcgcgccgg ccgacgtgct gcgcgaccgc gatacgctgg tggatgggga cgcgttgttg
     1441 ccgctggtca ttcggcgcgc ggctcgcgaa ccgctggcgc ttatcctggg gaggcgcgaa
     1501 ttctggagcc tcgacttcgc ggtgtcgccg gcgacgttga tcccgcgccc ggagtcggag
     1561 accctgattg aggcggcact ggcagccttt gcggggcgtg atccgcccga tcgtgtgctg
     1621 gatctgggca ccggaaccgg ttgcctgctg ctggcggcgc tgtcggagtt ccggtcggca
     1681 tttggcgttg gtgtggaccg ttcggctgag gccgccccac tagccaaagc caacgccagt
     1741 gcgctcggtc tggctggccg tgctgcattc gtatgtgctg attgggccgg tgccctcggc
     1801 gctcgcttcg atcttatctt gtgcaacccg ccttacatcc ccacgtcatg tttaccgggc
     1861 ctgatgccgg acgtggcaga gcatgagcca tggtctgccc tggatggcgg ttccgacgga
     1921 tatgctgcct acaggcacct gcttcccgag atcctccgcc tcctgcaacc ggacggcgtt
     1981 gcggtgctgg agttggggct ggaccaggcc gacgcagtgg gcgatatggc gcggcggatg
     2041 ggtctagtgg tggatttccg caacgatctc tcgggaatcg aacgggccgt gctgctgcgg
     2101 cggacatcgc catgaaaaaa ccgtttggca gcgcgaggcg gtcggtctag ctttctggtt
     2161 ggcagggtca gccgcacggc ggccgccccg ggcaatctgg tcgaaccgct cgtcgtctgc
     2221 tcaggtggat ggggctatcg gacagggggc cggccggaac caatgccgat ggattgaagc
     2281 ctggctgaga ggggcaagcc ccgagagacg cccgcgggtg gaactgcgtg catctccgtg
     2341 ttccctcccg ccaatgcaaa gagaaggcgt aacggcgata gtatgaacat gaaacgtatg
     2401 cgtggacgcg gccatcgacc tggtggcggc ggcggtggtg gtggcctgcg gcaccacgga
     2461 ggaggtagcg gcagcgggat tccgctcaat cgcaaccatg tgttcgacag cagcggcccc
     2521 gatctgcgga ttcgcggcac cgcgcagcag ttgttcgaga aatatctgca gctcgggcgg
     2581 gatgcgacca gtggcggcga tcgcgtgatg gccgagggct atttccagca tgcggaacac
     2641 tatttccgca ttctgaacgc gatgaaccag gcggcgcagc agagccagca gcagaatggc
     2701 ggtcagcgcc ggccgtacaa tggtgaggag ggtgggcaac cctctgatca gcctcccgag
     2761 gaagctgagg tgcgttcgcc cgcaggcgtg ggcgagcagc ccgaggcgcg cgaaatcccg
     2821 atcgccgcgg cgcctcccga agcgtaggcc gtaccgcact ggcagcgtcc caccgcttcg
     2881 cgcggcgggg gcggccatgt atggtgcgcc cgtcgcaggg gagaaaacgc gaatgcgctg
     2941 ggcacgcttc gaacagaacg gctcgccggc ttatggcgtc gtcgaaggcg atgagatcat
     3001 tccggtgcgc ggctcgccgt tcgagatgtg ggagcgcacg gcagcgcgcc ttccgatcgc
     3061 cagcgtgaag ctgctggtgc cagtggttcc cttgacgttc tatgcggctg ggttgaacta
     3121 cgcggagcat gtgcgcgagg cggccgagaa gttggggttg aaggtcgagc tgccgacggc
     3181 ggcagatgcc ggctatcgcg ccaacaacgc gctgatcgcg cagggcgagg cgatcgttat
     3241 tcctgaagat gcgaccgaac aggtgcagta cgagggtgaa ctcgtcgcgg tgatcgggcg
     3301 caagtgcaag catctggcgc gcgagaatgc gctgtcggcg gtgctgggct atacgatcgg
     3361 caacgatgtc agcgagcgta cctggcagaa aagcgaccgc acgctgtggc gcgcgaagaa
     3421 caccgatacg ttcaagccga tggggccttg gatcgagacc aatgtgaagc tcgatgagct
     3481 ggtcacgcgc gtgcggctga acggcgagga acggagcgtg ttcccgacca atcacatggt
     3541 gttcggtgtg gtcgattttc tcgttgcgat gacgaagtac ctgacgctgt atccgggcga
     3601 cgtgctgtgg atgggcaccg aaggtgccac cgccaatatg aagcacggcg atgtctgcga
     3661 ggtggagatc agcggcatcg gcacgttgcg caatccggtg gtgcgggcga gctgaagggc
     3721 cactggcgcg aggcagtgaa ttccgtgacg gcgtccatgg ccgtccggct tgaactgatt
     3781 ggtggaactg cgcgatccac gtcctttctt ccaatgggaa agacgtggat gccgggacaa
     3841 agtcccggca tgacgaaatg caacgacgct cgcatcttgc cgacagtatc tgtgttcaga
     3901 gcaccggaca gtcgtgggga gcgccggata atgaccgggt gcattgaacc gagtagggtg
     3961 ttgcgatcat gagccaggag gcgagcatgc aacgcgacgt ggtcgtggtt tccggcgtac
     4021 gcacggcgat tggcgatttc gggggcgggt tgaaggatgt gtcgccgact gatctcggcg
     4081 cgcaggtggt gcgcgaggcg ttgtcgcgcg ccagcgtgga tggcgcggag gttgggcagg
     4141 ttgtgttcgg caacgtgatc cataccgagc ctaaggacat gtacctcgcg cgggttgcgg
     4201 cgatgaatgc gggcgttgcc aaggaagcgc cggcgctgac gctgaaccgg ctgtgcgggt
     4261 ccgggctgca ggctgtggtt tcggcggcgc agacgatcat gctgggtgac agcgagatcg
     4321 cggtggccgg tggcgccgag agcatgagcc gggcgccgta cctggcgcag gggatgcgct
     4381 ggggcgcgag gatgggtgat gcggcgatga tcgacatgat gctcgccgcg ctgcacgatc
     4441 cgttcgacaa tattcacatg ggcgagacgg ccgagaatct tgccgagagc caccagatca
     4501 cgcgcggcga ccaggatgcg ctggcgctgg aatcgcatcg gcgtgcatcg cgcgcgatcg
     4561 cggaggggcg gttcaagcag cagatattgc cgatcaagct gaagacgcgc cgtggcgaga
     4621 cgattgtgga tacggatgaa catccgcgcg cggatcagac cgaggacgcc ctggcgaagc
     4681 tgcgaccggt gttccggaag gatgggacag tgaccgcggg gaatgcgtcg gggatcaatg
     4741 atggcgcggc ggctgtggtg ctgatggagc gtgccacggc ggcgaagcgc ggggtgaagc
     4801 cgatgggacg gctggtggct tatgcgcatg cgggtgtcga tccgaagatc atgggtattg
     4861 ggccggtgcc ggcgacgcgg ctcgcgttgc agcgggcggg tctggccgtt ggcgatatcg
     4921 atgtgatcga gagcaacgag gcgttcgccg cgcaggcgtg tgccgtggcc aaggatcttg
     4981 ggttcgatcc ggcgaagacc aatccgaatg ggtctggcat ttcgcttggc catccggtgg
     5041 gggcgacggg gtgcatcctg acggtgaagg cgctgtacga gctggagcgg attggcggac
     5101 gttatgctct ggtcactatg tgcattggcg gcgggcaggg tatcgcggcg gtgttcgaga
     5161 ggctgtgagg cggggcgctt ctgaggggca aagccaatgg cgggcgggca gcgaggcagc
     5221 tcggctgtct gccggtgcgg tgagacggcg atcgagttga gcggtgagcc gatccaaagc
     5281 gtgatctgtt tttgtgagag ctgccggacc gccgcgcgcg ggttcgaacg ggacctgggt
     5341 gctccaccta ccgtcaatgc ggatggcggc gtgcattact gcctgtaccg gaaggaccgg
     5401 gtgaagattg cgcgtggtgc gcaccacctg cgggaattcc gtctgaagcc ggactcgccg
     5461 acgcgccgcg tggtggcgag ctgctgcagc tcgccgatgt tcgtggaatt cacccgtgga
     5521 cattggctga cgatgttcct ggatcggctg tcggggcagg cgccggagcc gcagatgtgc
     5581 gtgatgacga agtacaggcc ggaaggtttg aagctgccgc gcggcatccc tgcctatggt
     5641 acgttaccgc ctagtctcat gatcaagctg ctcgccgcct gggcggcgat gggattccgc
     5701 cgtccgacga ttgtctggtg aagatcgcgg tagtggcaga gcccaatgaa tacgactcgt
     5761 tgctcgacgg tcgaaggacg tggatgtcga ccttcgtcgg catgacgcta accgccaatc
     5821 gcggtcccgc tcgtggtcgg tgacccccgg ccatcggaca ccagaggcac caatgttaat
     5881 gttcctgcgg agcgtgatgg ccggctcaag gccggccacg acgtgagagg ctatccgact
     5941 gctgcgagaa cctgatcgca gacgtgggcc atgttctctt ccgacatgtc gggatggatc
     6001 ggcagggcga gaattcgttc ggccaggtct tccgagacgg ggaggtggct gccgtcgtgg
     6061 tggtcctggt aggccggctg tgtgtgcagc ggccggggat agtagatggc ggtcgggaca
     6121 ccgttggcgc gcaaggacgc ctgcacgtgg tcgcgcgttg ccgcgtctcg taggaggatc
     6181 gcgtagattg cccaggcact ggtgctgtcc ggcacgcgcg ccggtgttgt gaccgcattg
     6241 ccgaggcggg aatcgtagta gcgcgcgatg cgctcgcgga tggccagctc attggggaac
     6301 acggtcatct tcgccagcaa gacagcggcc tgcattgaat ccaggcggcc gttcatgccg
     6361 gtgtgcagga cctcgtagcg cgacatgcct tcgccatggg tgcgcaggct gcgatagagg
     6421 gcggcacggt cgtcgctttc ggtgaagagc gcgccgccgt cgccgtaggc gcccagtggc
     6481 tttgagggga agaagctggt ggcggttgca tcggccgcgg cacccagggg gcggccttgc
     6541 tgtgtacagc cgaagctctg ggccaggtcg tcgactgtga agaggtcctc gcggcgggcg
     6601 atggctgcga gggctggcca gtcggctggc tggccgaaca gatctacgcc gatgagtgcg
     6661 cgcgggcgga gcttgccggc gcggcggatc tcggtgattc gctgttccag atgagccggg
     6721 tcgatctgga aggtgtgggg atcgacatcg acgaagaccg gtgtcgcgcc gaggaccagg
     6781 gggacttcgg cggtggcggt gtaggtgaag gctggcagaa agaccgcgtc gcctcggcca
     6841 acgttttccg ccatcagggc gatctgaagc gcatcggtgc cggagctgac tgtgacgcaa
     6901 tggcgggcgc cgcagaacgc ggccagttcg cgttccagtt cggcgacctc gggtcccagg
     6961 atgaactggc agtgggccag cacgccgtcg agccgacggc gcaattctac cgcgatccgc
     7021 gcctgctggg ccttcaggtc gataaacggc atcatgggcg gttcaccacg ggcatcagtc
     7081 cttcggggtt gtgttcgaac tcgggtacca gcctaccgag caaggtaagt gccaggcgcg
     7141 actggccacc tcggcaggcg ccggcgatct cgtcgatggc gcggccgacg atggcggggt
     7201 cggcggtgcg gggtgcggcc atcagaaggc cggggtaatt cgtgggtacc gggggttcgg
     7261 tgccgtggaa gagttcctcg tgcagctttt caccagggcg gaggccggtg aagcggattt
     7321 ccacatcgcg atccgggacg aggccagcga ggcggaccat ctggcgcgcc agatcgacga
     7381 tcttcacggg ggggcccatg tcgaggacga aaatgccgcc ttcgtcgcgg gccgggagat
     7441 cggcgttgcc tacgcgcacc acgcttgcct gaagcaccag tcccaccgcc tcgcgcaccg
     7501 tcatgaagta gcgctgcatg tcaggatgcg tgacggtcaa cgggccgccg cgttccaatt
     7561 ggcgctggaa cagcgggacc accgagccgg tgctgcccag cacattgccg aagcggacgg
     7621 tgacacagcg catgccatgg gaggcgatgt ccagcccctg gcagtacatt tcggcgaggc
     7681 gcttggaggc tcccatcacc gacgtggggt tcaccgcctt gtcggtcgag atcagcacca
     7741 tggcgagcga gccgacggca cgggcggcgt ccgcgacgtg gcgggtgccg gcgacgttgg
     7801 tcagcaggcc ttccagcggg ttggcctcga ccatcgggac gtgtttcagg gcggcggcgt
     7861 ggaagaccag ttcggggcgc cattgctcga agaccgcgcg gatgcggcct tcgtcgcgga
     7921 tgtcggccag cagggattcg cgcgggacgt gcgggtggcg ctcggcgagc tccaggtcga
     7981 tcagccagag ggcgtattcg ccgttgtcga cgaggatcag gcgttcgggt ccgagtgcgg
     8041 cgacctggcg ggccaactcg ctgccgatgg tgccaccggc gccggtgacg atgacgcggc
     8101 ggccctggat cagccgggcc atgccctcgc gatcgagggg gacctgggca cgattcagca
     8161 ggtcctcgat ggccatgggg cgcagttcga gcttgtcgcc gcgttcggtc gcggtatcca
     8221 gggcggtcgg gcggggcgcg cggcggatct gcaa
//
LOCUS       DATGEM010000301         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_985619, whole genome shotgun sequence.
ACCESSION   DATGEM010000301 DATGEM010000000
VERSION     DATGEM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985619"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VKQ27_09615"
     CDS             <1..141
                     /locus_tag="VKQ27_09615"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLY89231.1"
                     /translation="EPLTGAAQMALKMREDVVTDGGYPEKVLANAPERAGDFFVVPKV
                     IE"
     gene            141..1628
                     /gene="gatA"
                     /locus_tag="VKQ27_09620"
     CDS             141..1628
                     /gene="gatA"
                     /locus_tag="VKQ27_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HLY89232.1"
                     /translation="MAEALTDLTIAAARTGLRKREFTSRELTEAHLAAIEALNPRLNA
                     FITVTGERALAQADAADAAIARSELAPLTGIPLAIKDLFCTEGVRTTAGSRILEPFVP
                     PYESTVSANLLRDGAVFLGKANMDEFAMGSSNMTSAYGPVANPWKRRQDAEAVLVPGG
                     SSGGSASAVAARIAMGATGTDTGGSIRQPASFCGIAGIKPTYGRCSRWGVVAFASSLD
                     QAGPFARTVEDCAILLGSMAGHDPKDSTSADVPVPDFVAACRRDVKGLRIGIPREYRL
                     DGMPEDIERLWRQGVAWLRDAGAEVVDVSLPHTKYGLTTYYIVAPAECSSNLARYDGV
                     RFGRREPGVDLRDLYERTRAAGFGAEVRRRVLIGTYVLSAGYYDAYYVRAQKVRALIL
                     RDFTEAFSQVDALLAPTAPSAAFAQGDNMDDPIKMYLNDAFTIPGSMAGVPGMSVPAG
                     LDVNGLPLGLQVIGKPFDEETVFAVAAAIERAAGITALPQLRAGA"
     gene            1632..>2689
                     /gene="gatB"
                     /locus_tag="VKQ27_09625"
     CDS             1632..>2689
                     /gene="gatB"
                     /locus_tag="VKQ27_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLY89233.1"
                     /translation="MSYALEGSTGTWEIVVGLEVHAQVISKAKLFSGAATDFGAPPNT
                     QVSFIDAAFPGMLPVINRECVAQAVRTGLGLNAHIHLVSRFDRKNYFYADLPAGYQIS
                     QYQHPIVGEGRIEIELADGSTRDIGITRLHLEQDAGKSLHDQHPTRSYIDLNRSGVAL
                     MEIVSEPDIRSPEEAGAYLRKLRSIMRYLGTCDGNMDEGSMRADVNVSVRKHGEPYRT
                     RCEVKNVNSIRFVMQAIEAEAKRQIEVWESGSAVEQETRLFDPGRGMTRSMRSKEDAH
                     DYRYFPDPDLLPLVLEEGWVQALKSKLPELPDAKKRRFVQDFGLPAYDAGVLVAEQST
                     ADFYETVAKGRDAKIAAN"
BASE COUNT          477 a          793 c          930 g          489 t
ORIGIN      
        1 gagccgctga caggcgccgc gcagatggcg ctgaagatgc gcgaggacgt ggtgacggat
       61 ggcggctatc cagagaaagt gctggccaac gcgccggaaa gggcagggga cttttttgtc
      121 gtgcccaagg tcatcgagtg atggccgagg ccctgaccga tctgacgatt gccgcggccc
      181 gcacggggct gcggaaacgc gaattcacat cgcgcgaact gaccgaagcg catttggcgg
      241 cgatcgaggc gctgaacccg cggctcaacg ccttcatcac ggtaacaggc gagcgcgctc
      301 tggcacaggc cgacgcggcc gatgcggcga tcgcgcgcag cgagttggcg ccgctgacgg
      361 gcataccact ggcgatcaag gacctgttct gcactgaggg cgtgcggact accgccggca
      421 gccgcatcct ggagccgttc gtgccgcctt atgagagcac ggtcagcgcc aatctgctgc
      481 gcgacggcgc ggtgttcctg ggcaaggcga acatggacga attcgccatg ggctcgtcca
      541 acatgaccag cgcctatgga ccggtggcga atccgtggaa gcggcggcag gacgccgagg
      601 cagtgctcgt gccgggtggt tcttccggcg gctccgcgtc tgccgtggcg gcgcggatcg
      661 ccatgggtgc gacgggtacc gataccggcg gctcgatccg ccagccggcg tcgttctgcg
      721 gcattgccgg catcaaaccg acctacgggc gttgttcccg ctggggggtt gtcgcgtttg
      781 cgtcgtcgct cgatcaggct gggccgttcg cccgcacggt cgaggattgc gcgatcctgc
      841 ttggttcgat ggcggggcac gatccgaagg attcgacgtc tgccgatgtg ccagtgcctg
      901 attttgtcgc ggcgtgccgg cgcgacgtca aagggctgcg cattgggata ccgcgcgaat
      961 atcggctcga tggcatgccg gaggatatcg agcggctttg gcggcagggc gtcgcgtggt
     1021 tgcgcgatgc cggtgccgaa gttgtggacg tttcattgcc gcacacgaaa tacggactga
     1081 cgacctacta tattgtcgct cccgcggaat gttcgtcgaa cctggcccgt tatgacggcg
     1141 tgcggttcgg tcgacgcgag cctggcgttg acttgcgtga tctctacgag cgcacgcgcg
     1201 cggcaggttt cggcgcggag gtgcggcggc gcgtgctgat cggcacgtat gtgctgtcgg
     1261 ctggctatta cgacgcttat tacgtgaggg cgcagaaggt gcgagcgctg atcctgcgtg
     1321 atttcaccga ggcattttcg caggtcgatg cgttgctggc gccaaccgca ccgtccgccg
     1381 ccttcgcgca aggcgacaac atggacgatc cgatcaagat gtacctgaac gacgcattca
     1441 ctattccggg cagcatggcc ggggtgccgg gaatgtcggt cccggccggt ctcgacgtga
     1501 atggattgcc gctcgggttg caggtgatcg gcaaaccatt cgacgaagaa accgtgttcg
     1561 ccgtggccgc ggcgatcgag cgggccgccg gcattaccgc gttaccgcaa ttgcgggcgg
     1621 gagcctgaac catgagttat gcactcgaag gatccaccgg tacctgggag atcgtcgtgg
     1681 gcctcgaggt ccacgcgcag gtgatcagca aggcgaagct gttctccggc gctgccaccg
     1741 atttcggcgc gccacccaac acgcaggtga gctttatcga cgccgcgttc cccggcatgt
     1801 tgccggtgat caatcgcgaa tgcgtggcgc aggcagtgcg cacagggctt ggattgaacg
     1861 cgcacatcca tctggtcagc cggttcgatc ggaagaacta cttctacgcc gacctgccgg
     1921 ctggttatca gatcagtcag tatcagcatc cgatcgtcgg cgaaggtcgc atcgagatcg
     1981 agctcgccga cggctcaacg cgcgacatcg gtattacgcg cctgcatctc gagcaggatg
     2041 ccgggaagtc gctgcacgac cagcatccga ccaggtccta catcgatctg aaccgctccg
     2101 gcgtcgcact gatggagatt gtatcggagc cggacatccg ttcccccgag gaggccggcg
     2161 cgtatctacg caagctgcgt tccatcatgc gttacctggg gacgtgcgac gggaacatgg
     2221 acgaagggtc catgcgcgcc gacgtcaacg tgtctgtgcg caagcatggc gagccgtatc
     2281 gcacgcggtg cgaagtgaag aacgtgaatt ccatccgctt cgtgatgcag gcgatcgaag
     2341 cggaagcgaa gcgccagatt gaggtgtggg aaagcggcag tgcggtcgaa caggagacgc
     2401 gtctgttcga tcccggtcgc ggcatgacgc gctccatgcg cagcaaggaa gacgcccatg
     2461 actatcgcta tttccccgat ccggacctgc tgcccttggt gctggaggag gggtgggtgc
     2521 aggcgctgaa gtcgaagctg ccggagctgc ccgacgcgaa gaagcgccgc ttcgtgcagg
     2581 attttggctt gccggcctac gatgcaggcg tgctggtcgc cgagcagtcg acggcggact
     2641 tctatgagac tgtggcgaaa ggacgcgacg cgaagatcgc cgccaactg
//
LOCUS       DATGEM010000302         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1398186, whole genome shotgun sequence.
ACCESSION   DATGEM010000302 DATGEM010000000
VERSION     DATGEM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398186"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1163)
                     /locus_tag="VKQ27_09630"
     CDS             complement(186..1163)
                     /locus_tag="VKQ27_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89234.1"
                     /translation="MSAATKQSRTHLKAAAELSPVEPNAGSADTSSQNIFTQPEQNQL
                     PQLTNCVIITTNMNHAAPTSAASPQDPLQDRRVRPVPNNIEVLLHAVGVLLTYGRHLI
                     DTVRQRATAPNFNAIAACFGTSNLSTIVAHLNRGLLRAAALERLLLARAATGRDIDVI
                     KRHTWTTLEPGADPAEDTQPEPPAAQPSITHKRASRPSRPAGWDDPELFMPTLQELER
                     QVRRHPIGRTILHICLDLGVVPGFCHSAFWNELFELITHFGSSVSKVMEEKTRRRQAF
                     AKEQDRNPKASWDWLSLSRDAPRQILGFFIGEPPVNPLDPAAAIATGPP"
     gene            1259..2782
                     /gene="guaB"
                     /locus_tag="VKQ27_09635"
     CDS             1259..2782
                     /gene="guaB"
                     /locus_tag="VKQ27_09635"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLY89235.1"
                     /translation="MAFDTPHSHTSSRSSGLSPEDRRIEEALAFDDVLLVPGYSQVLP
                     SSTDTRTRLTRTISLNIPIISAAMDTVTEASMAIAMAQQGGMGVIHKNLGPEEQAAQV
                     RRVKKYESGMVVNPLTIHPDQTLADVTALMASNRISGIPVVERDTNRLVGIITHRDVR
                     FATDPHQKVYELMTRENLVTVTAGVSPDEARRLLHRHRIEKLLVVDDAYRCVGLITVK
                     DMDKAETHPLANKDELGRLRVAAATGVGPDGQARSRALIEAEVDVIVVDTAHGHSRGV
                     LEAVEAIKKLSNAVQVIAGNVATPEGALALIEAGADGVKIGIGPGSICTTRVVAGVGV
                     PQFTAVLETAAACREAGVPGIADGGMRTSGDIVKAIAAGADAVMIGSLLAGTDEAPGE
                     VFLYQGRSYKSYRGMGSIGAMARGSADRYFQQDIKDQLKLVPEGVEGMVPYKGPVAQA
                     IHQLVGGLKAGMGYTGSADIGELQRSSRFRRVTGAGLRESHVHDVAISREAPNYRQD"
     gene            2787..3443
                     /locus_tag="VKQ27_09640"
     CDS             2787..3443
                     /locus_tag="VKQ27_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HLY89236.1"
                     /translation="MARAAVFDAYGTLLNVHAAMAKHAARLGPDWERMSADWRTKQIE
                     YSWVRSLAGAAHHRDFWSLTREALAFVATRYGIVDEALLADVLAAYRRLDAYPEVPAM
                     LGSLRDRGVPRAILSNGEPGMLADAVRGAGIAELLDDVLSVESVGVFKPDPRVYRLVT
                     ERFGLPPEAIDFYSSNPWDAFGARAFGFRVSWVNRSRQPDEYGLRGHVAELADLSAVT
                     "
     gene            3440..4642
                     /locus_tag="VKQ27_09645"
     CDS             3440..4642
                     /locus_tag="VKQ27_09645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HLY89237.1"
                     /translation="MTPAARTESAIELLGLVLEGERPADAVANDYFRTRRFIGSGDRR
                     AVAERTWRVLRGYRRLSWWLRDQPTPRLLIAASLVLDGGTLDDFTGGRFAPEALAPSE
                     RAVLRRLEGRSLDHPSMPDGVRLEVPDWIVGRIAPEDLRALLEPATLDLRVNLLKGTR
                     DEARAALAGEGVETVLTPLSPWGLRVDGRRQVTTGAAFRGGLVEIQDEGSQLVAALVD
                     ARPSMRVADLCAGAGGKTLALAMTMQNRGQLIACDVSAARLDNAVQRLRRAGVHNAER
                     HLLVRGDKWIKRHAEQFDRVLVDAPCTGTGTWRRNPDARLRLGEDDMAELLPKQMAIL
                     DQAGSLVRKGGRLVYATCSLLAEENEDQVTAFLSRHSGFAQTDVLALSPARHGTDGFF
                     AAVLERGA"
     gene            4797..>5007
                     /locus_tag="VKQ27_09650"
     CDS             4797..>5007
                     /locus_tag="VKQ27_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89238.1"
                     /translation="MISVRRARAADAISIGSVHVAAWRSAYPGILPDDFLARLSVPRQ
                     AAHYDAAIRSSSTGVFVASASGNDVP"
BASE COUNT          778 a         1430 c         1904 g          895 t
ORIGIN      
        1 tggcatggtc gctgcccatc tcgtccgcca tgtgcgggtt ctcgcgttgg tggggctgat
       61 gccactatgg cagaccgatc gccctcggca acaagggcag aggcatggca cgcttcgccc
      121 gaaggagcga cgggtcggtc ctactcagag gggtctgtgt tggctggcac tgtattgcgg
      181 gtgcgtcagg gcgggccagt ggcgatggcg gccgcagggt cgagcgggtt caccggtggc
      241 tcgccgatga agaagccgag gatctggcgg ggcgcatcgc ggctcaggct gagccagtcc
      301 cagctggctt tggggttgcg gtcctgctcc ttggcgaagg cctgccggcg gcgggttttt
      361 tcctccatga ccttgctgac gctgctgccg aaatgggtga tgagttcgaa cagttcgttc
      421 cagaaggcgc tgtggcagaa gcctgggacg acgcccagat cgagacagat atggaggatg
      481 gtgcggccga tcgggtgacg gcgcacctgg cgctccaatt cctgcaatgt gggcatgaag
      541 agttcgggat catcccagcc cgcgggacgc gaggggcgtg aggcgcgctt gtgcgtgatg
      601 gagggctggg ccgctggcgg ctcgggctgg gtgtcttctg ccggatcggc ccctggttcc
      661 agcgttgtcc aggtgtggcg cttgatgacg tcgatgtcgc ggccggtggc ggcgcgggcg
      721 aggagcaggc gctcgagcgc cgcggcacgc aggaggcccc ggttcaggtg ggcgacgatc
      781 gtcgagaggt tgctggtgcc gaagcacgcg gcgatggcgt tgaaattggg cgcggtggcg
      841 cgctggcgga cagtgtcgat gaggtggcgg ccataggtga gcaggacgcc gacggcgtgg
      901 aggagtacct cgatgttgtt ggggaccggc cggacacggc gatcctgtaa gggatcttgg
      961 ggggaggcag cagacgtggg tgcggcgtgg ttcatgttgg ttgttataat aacgcaattc
     1021 gttagttgcg gcaactgatt ttgttcgggt tgggtgaaaa tattctgact ggaggtatcg
     1081 gcggagccgg cgttcggttc gacggggctg agctcggcag cagctttcaa atgcgttctg
     1141 gattgcttcg tcgctgcact catcgcaatg acagtgctgg ggtctaactg acgcgtgctt
     1201 gcagactccc acccaggcgc ctatgtgggg ccgccaaggt agcggaaagg cccgccccat
     1261 ggcgttcgac accccccaca gtcacacctc cagcaggtcg tcgggactct cgcccgagga
     1321 caggcgcatc gaagaagcgc ttgccttcga tgacgtgctg ctggtgccgg gctactccca
     1381 ggtgctgccc tcgtccactg atacgcggac gcggctgacc aggacgatct cgctgaatat
     1441 accgatcatt tcagcggcta tggacaccgt taccgaggcc tcgatggcga ttgccatggc
     1501 gcagcagggc ggaatggggg tgatccacaa gaacctcggg cccgaggaac aggcagcaca
     1561 ggtccgtcgg gtgaagaagt atgaatccgg catggtggtg aatccgctca ccatccaccc
     1621 tgaccagacg ctggcggacg tcaccgcgct gatggcaagc aacaggatca gcggcattcc
     1681 ggtggtggaa cgggacacca atcgtctggt gggcatcatc acccatcgcg acgtgcgttt
     1741 tgccaccgac ccgcatcaga aagtctacga actgatgacg cgggagaacc tggtgacggt
     1801 caccgccggc gtctcgccgg atgaggcccg ccggctgctg caccggcatc ggatcgagaa
     1861 gctgctggtg gtggatgatg catacagatg cgtcgggctg atcactgtga aggacatgga
     1921 caaggcagag acgcatccgc ttgccaacaa ggatgagctc ggccggttgc gcgtggccgc
     1981 tgcgaccggt gtggggccgg atggccaggc gcggtctcgc gcgctgatcg aggcggaagt
     2041 ggacgtgatt gtggtggata ccgcgcacgg tcactcacgc ggcgtgctgg aggcggtgga
     2101 ggcgatcaag aagctttcca acgcagtgca ggtcatcgcg ggcaatgttg ccactccgga
     2161 aggtgcgttg gcgctgatcg aggccggtgc cgatggagtg aagatcggga tcgggccggg
     2221 ttcgatctgc acgacgcggg tggtggccgg ggtcggtgtg ccgcagttca ccgcggtgct
     2281 ggagacggcg gcggcatgcc gcgaggctgg tgtgccgggg atcgccgatg gcggcatgcg
     2341 cacgtcgggc gacatcgtga aggcgatcgc ggcgggggcg gacgcggtga tgatcggctc
     2401 gctgctggcc ggcacggatg aggcgcccgg cgaggtgttc ctatatcagg gccggtcgta
     2461 caagtcgtat cgcgggatgg gcagcatcgg cgccatggcg cgcggttcgg cggaccgcta
     2521 cttccagcag gacatcaagg accagttgaa gctggtgccg gaaggcgtgg agggcatggt
     2581 gccctacaag gggccggtgg cgcaggcgat ccaccagttg gtgggcgggc tgaaggccgg
     2641 catgggctac accggcagcg cagacatcgg cgaattgcag cgaagcagcc ggttccgccg
     2701 ggtcaccggg gcgggactgc gcgagagcca tgtgcatgac gtggcgatca gccgcgaggc
     2761 gccgaactac cggcaggatt gaacctatgg cacgcgccgc ggtgttcgac gcctatggca
     2821 cattgctgaa cgtgcatgcg gcgatggcga agcatgcagc gcgactgggg ccggactggg
     2881 agcgcatgag cgcggactgg cgcaccaagc agatcgaata cagctgggtc cgcagtctcg
     2941 ccggagccgc gcaccatcgc gacttctggt cgctgacgcg ggaggcgctg gcgttcgtgg
     3001 cgacgcggta cgggatcgtg gatgaggcgc tgttggctga cgtgcttgcg gcctaccggc
     3061 gtctcgacgc gtatcccgag gtgccggcga tgctgggatc gctgcgcgac cgcggcgtac
     3121 cgcgcgcgat cctttcgaac ggagagcctg ggatgctggc ggatgcggtg cgcggcgctg
     3181 gcatcgcgga gctgctggac gacgtgctga gcgtggaaag cgttggcgtg ttcaagcccg
     3241 atccgcgcgt ctatcggctg gtgacggaac ggttcggcct gccgccggag gcgatcgatt
     3301 tctactcctc caacccgtgg gatgcgtttg gcgcgcgggc attcggcttc cgggtatcgt
     3361 gggtcaaccg gtcgcgccag cccgacgaat atgggctgcg cggacacgtc gcggaactgg
     3421 cggacctgtc ggctgtcacg tgacgcctgc cgcgaggacg gaatctgcca ttgaattgct
     3481 gggtctcgtc ctcgagggtg agcgcccggc cgatgccgtt gccaacgact acttccgcac
     3541 ccggcgcttc attggttcgg gcgatcgacg tgcggtcgcc gagcgcacct ggcgcgtgtt
     3601 gcggggctat cggcggctga gctggtggct gcgggatcag ccgacgcccc ggctgctgat
     3661 cgctgcttca ctggtgctgg acggtgggac gctcgacgac ttcaccggcg ggcgcttcgc
     3721 gcctgaggcg ctggctccct cggagcgcgc ggttctgcgc cggctggaag ggcgctcgct
     3781 ggatcatccg tcgatgccgg acggcgtgcg gctggaggtg ccggactgga ttgtcgggcg
     3841 cattgccccg gaggatctgc gcgcgctgct ggaaccggcg acactcgacc tgcgcgtcaa
     3901 tctgctgaag ggcacgcgcg acgaggctcg tgccgcgctt gccggcgagg gcgtggagac
     3961 ggtgcttacg ccgctctcgc cgtggggcct gcgcgtcgac gggcggcgtc aggtcacgac
     4021 cggcgcggcg tttcgcggcg gcctggtgga aatccaggac gaaggcagcc agcttgtcgc
     4081 ggcgctggtc gatgcgcgtc ccagcatgcg cgtggctgat ctatgtgccg gggcgggtgg
     4141 caagacactg gcgctggcga tgacgatgca gaaccgcggg cagttgatcg cctgcgacgt
     4201 gtcggcggca cggctcgata atgcggtgca gcggctgcgc agggcggggg tgcacaatgc
     4261 ggagcggcac ttgctggtgc gcggcgacaa atggatcaag cggcacgccg aacagttcga
     4321 tcgggtgctg gtcgatgcgc cttgcaccgg gaccggcacg tggcggcgca atccggacgc
     4381 gcggcttcgc ctgggcgaag atgacatggc tgaactgctg ccgaaacaga tggcaattct
     4441 cgatcaggct ggctcgctgg ttcgcaaagg cggccgtttg gtctacgcta cctgctcact
     4501 gctggctgag gaaaacgagg accaggtgac tgcctttctt tcgcgccatt caggcttcgc
     4561 ccagacagat gttctggcgc tgtcgccggc gcggcatggc actgatgggt tcttcgcggc
     4621 ggtgctggag cggggtgcgt gatcgccacg ccggcgactg aaggcgtggg tgctcgcgcc
     4681 gagcgcgagc atgacggacg ttgcggttgg ccgggctgtc gcctggatgc atcgatgatg
     4741 gccgggtcga gcccggccat gacgagtgag gaccgggctg tcgcctggag gtacccgtga
     4801 tcagcgtgcg gcgtgcacgg gcggcggatg ccatttccat cggctcggta catgtcgcgg
     4861 cgtggcgcag cgcgtatccg ggcatccttc ccgacgattt tctggcacgg ttgtcggtgc
     4921 cgcgtcaggc ggcgcattac gatgcggcga tccggtcgtc ttcgaccggt gtgttcgtgg
     4981 catcggcatc cggcaacgac gtgccgc
//
LOCUS       DATGEM010000303         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_371483, whole genome shotgun sequence.
ACCESSION   DATGEM010000303 DATGEM010000000
VERSION     DATGEM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371483"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1575
                     /locus_tag="VKQ27_09655"
     CDS             409..1575
                     /locus_tag="VKQ27_09655"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLY89239.1"
                     /translation="MPRQRKPNDLSRSLIALDMDTTLIAVVELSLSSWLVAGIVPGVE
                     RQPLKKLKTDENALLALLNRWRDEAVRSGHKISRIAVAFEAGRDGFWLARWLKARGIE
                     AHVIHASSVAVSREHRRAKTDRLDTELLKRAFVGWLRGERDHCKTAAIPDLREEDAKR
                     PSRERESLVGEQSRLVNRMKATLIRLGIRGFNPKLRHAEQRLETLRTPEGEPIPANTL
                     AELKRDMQRRRFICDQIRSIEQERLDRLARAPDTEPHAMVRLLARVTGIGIETADLLV
                     HEVLLRNMRDRRAVARYGGLTGSPDRSGGTNREKGMARSGNARVRRGMIQLAWRFLLF
                     QKNSALAQWYRQRTEHAPKARKTMIVALARKLLIALWRLVREGVIPEGVAMRPA"
BASE COUNT          440 a          651 c          734 g          424 t
ORIGIN      
        1 ggaccggctc gatacggtgt ggtccgcttc ggtgctgccc gggtcgggag cttggttact
       61 tgcagtggtc tcgtcgggga cacggcctgc aggattgacg gagcttgcat gttttctgtc
      121 tctcgataag aggagcgcgc cgcaaggcga cgcgataacc cggcgacgtg cacccgcgtt
      181 caaaccaact ctgtttgggg tgttagcatc atccgagcct ggggatgctg cccttcgacg
      241 tttggaccta tcgtctcgat ctgcagcgta gagttaagag ctattaaagt ctgagcaaaa
      301 ttgatcactg cagatcgatc ggggctggtt ttgtgcagat tctatggggc gcgcggcgag
      361 gagattgtat ccatcggcgc ggatgtaaac cgagcgacgg agccccccat gccacggcag
      421 cgtaagccga atgaccttag ccgatccctg atagccctcg acatggatac gacgttgatt
      481 gccgtagtcg agttgagcct gtcgagctgg ttggttgccg gtatcgtacc cggcgtcgag
      541 cgccagccac ttaagaagtt gaagaccgat gagaatgctc tgcttgcgct gctcaatcgc
      601 tggcgcgatg aagcggtgag gtcgggacat aagatctcgc gcatcgccgt ggccttcgag
      661 gccggccgcg acggcttctg gttagcgcgc tggctcaaag cacgtggcat tgaggcgcat
      721 gtgattcacg cgtcgagcgt ggcggtgtcg cgcgagcatc ggcgggccaa gacggatcgg
      781 ctcgacaccg agctcttgaa gcgtgccttt gtgggctggc tgcggggcga gcgtgatcat
      841 tgcaagacgg ctgccattcc ggaccttcgc gaggaggacg ctaagcgacc aagccgcgag
      901 cgcgagagcc tggttggcga gcagagccgg ctcgtcaatc gtatgaaagc gacattgatc
      961 cggcttggga tccgcggctt caatccgaag ctgagacatg cggagcagcg gctcgagacg
     1021 ttgcgcacac ccgagggcga accaatcccg gccaacactc tggccgagct caagcgggac
     1081 atgcagcgac ggcggttcat ctgtgaccag atccggagca tcgagcagga gcgtcttgac
     1141 cgtttggcgc gtgcacccga cactgagcca cacgccatgg tgcgcctgct ggcacgcgtc
     1201 accggtatcg gcatcgagac cgccgacttg cttgtgcatg aggtgctgct gcggaacatg
     1261 cgcgatcgcc gggcggtcgc gcgttacgga gggctgacag gctcgcctga ccggagcggt
     1321 ggcacgaacc gcgagaaagg gatggcacga tccggcaatg cccgcgtgcg ccgcggcatg
     1381 atccaactag cgtggcggtt cctgctgttc cagaagaaca gtgctctggc gcaatggtat
     1441 cgtcagcgta ccgagcatgc accgaaagct cgcaagacca tgattgtggc attggcccgc
     1501 aagctcctga tcgcattgtg gcggctggtg cgggagggcg tgattccgga gggtgtcgcc
     1561 atgcgcccgg cgtgatgaac acgagctatt gaatatacgt cgccaatagc ctgacggttt
     1621 tcggctcgat ccgcaccacg gtgagggttc gccgatgacg gttcgaggtg gcggggtccc
     1681 gcaaaaacca tggctttcat gccgttgtgc agaatgggcc cgccgcctcg gagcttcgct
     1741 gccgatgcgc aagactgcat catggtcagg gtcgttgcgg cccgccgaat acaaggttgc
     1801 ggcacgatgt tcgtgcccga caagcgagct ccccctcgat tccgtcgcat cgcacttggt
     1861 acgagacacc actcaacgtc gatgaaagga agtcctcaaa atagaaatcc atagccgatc
     1921 agtcgcgccg aatgaaattc gcgcgccccc ggcaacgaat tatgatggct cggtttgcgc
     1981 attcgccgtc aagccccgcg ccttcggcgc gccgcaagcg gcttcggggc ttgaccgctc
     2041 agcgcaaccg agcaggaggg ctgctgtgcc gaatgcaatc tcatggcgcg tcaaaatgac
     2101 tcaacaacac ctttgcttgc tacttgacaa caggagcccc atacaagggc tgacaggctc
     2161 gcctgaccgg agcggtggca cgaaccgcga gaaagggatg gcacgatccg gcaatgcccg
     2221 cgtgcgccgc ggcatgatcc aactagcgt
//
LOCUS       DATGEM010000304         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1579048, whole genome shotgun sequence.
ACCESSION   DATGEM010000304 DATGEM010000000
VERSION     DATGEM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579048"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VKQ27_09660"
     CDS             <1..296
                     /locus_tag="VKQ27_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HLY89240.1"
                     /translation="IHPATRRALRARDKHCRWPGCDRPINWTSPHHIEFWGHGGETKP
                     PNLLSLCWFHHRLVHEGGWQVVKAGKEFRFIPPQQTSVLKEWRARGPGYRQAA"
     gene            complement(319..1491)
                     /locus_tag="VKQ27_09665"
     CDS             complement(319..1491)
                     /locus_tag="VKQ27_09665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89241.1"
                     /translation="MLGHLTVDSYVGVIPVLYPVLIGRFDLNFATVGLISLAYSGTAA
                     ISQPLFGVIADRYGTRFTGVALVWTAITFALVGFVPTFPLLVALACASGLGSGAFHPF
                     GALDVRALLPKRSRSAGMSIYVTAGTVGVAIGPLIGIGLFTWFGLHGTGWLVIPGIAT
                     GTYLLWRMRTAAPSSATERKTAPAYRQVVPMLAIAAVIGVMMSRSWTVSVFQTFTPTW
                     YRELGYGPGFYGPLVTTLVLSSAVGTVGCGALADRFGRRTVIIGTLVLSIPAILLYTL
                     FPGPWGFVTGIAIGFLAASTAPLMLLMAQQLMASRAGLASGLVMGLGFVTGALGVPIN
                     GAIGDAIGLQKSLMTHVILVVVTIGVAWLLPSEPDMEKYSAVAAPPAEALKPSVVT"
     gene            complement(1708..2334)
                     /locus_tag="VKQ27_09670"
     CDS             complement(1708..2334)
                     /locus_tag="VKQ27_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89242.1"
                     /translation="MFFLVVFSPNLPMYGDSLGDWGRWAPTHQSAARDAAALLLLAYL
                     LYMVFSLYVATLVRRSEPSTATGVQVATVAIAVKFAIEIMQIAVLVVPETGGSKDFGG
                     SMAQLGSVLSVLSLVPFAVFLVAVGVSALISRAVPIWLAWFTLGVAGIHVIAIVLGLA
                     GVSASPMLVAFGYVWFASIPAWPLVTSVALLVSALRRPGMGLRAATAS"
     gene            complement(2417..2893)
                     /locus_tag="VKQ27_09675"
     CDS             complement(2417..2893)
                     /locus_tag="VKQ27_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89243.1"
                     /translation="MAGGLLQVAGAAIPGTTWLTAGLSSLSHLLLLAGMLGLWTSGAM
                     GRGRIARAGTWITVISWAILAVAEFVDVVSTDVAVPLFSAATVGLPAGLILAGIATLR
                     TRLWKGWHRFIPLIAGLFVVLVVFPSFALPGHDFEYVIAAWGVCFVLLGAAMWAEA"
     gene            complement(2959..>3340)
                     /locus_tag="VKQ27_09680"
     CDS             complement(2959..>3340)
                     /locus_tag="VKQ27_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLY89244.1"
                     /translation="TFDFADTFSGQAAPSIQPINVRLGDLVRLHIVNHSRQSHPIHIH
                     GHVFSVLAKNGRTITGSPVHLDAILVGPGDSWDVGFVADNPGIWMLHCHVLIHAAGGM
                     SMTVNYEGISSPFTMGSRSGNIPE"
BASE COUNT          654 a         1106 c         1100 g          480 t
ORIGIN      
        1 cgatccaccc ggcgacgcgg cgggcactgc gagcacgaga caagcactgc cggtggccgg
       61 gttgcgaccg gccgatcaac tggacgagcc cgcatcacat cgagttctgg ggtcacggcg
      121 gcgagaccaa accgcccaac ctgctgtccc tgtgctggtt ccaccaccgg ctggtgcacg
      181 agggcggatg gcaggtggtc aaggccggca aggagtttcg cttcatcccg ccgcagcaga
      241 cgtcagtgct gaaagagtgg cgggcgcgcg gaccggggta tcgccaggcc gcttaggcgg
      301 ccgagccggt tcccggaatt acgtcacgac cgacggcttg agcgcctcag ccggcggcgc
      361 tgcgaccgcg gagtatttct ccatgtcggg ttcgctgggc agcagccacg cgacgccgat
      421 cgtcaccacg accaggatga cgtgcgtcat gagcgatttc tgcaggccga tcgcgtcacc
      481 gatggcgccg ttgatgggga cgccgagcgc gccggtgacg aagccgagtc ccatgaccaa
      541 ccccgaggcg agcccagcgc gtgaggccat gagctgctgc gccatcagca gcatgagagg
      601 cgctgtcgac gcggccagga atccgattgc gatgcccgtc acgaatcccc acgggccggg
      661 gaacagcgtg tacagcagga tcgccgggat gctgaggacc agcgtgccga tgatcacggt
      721 gcgcctgccg aagcgatccg ccagcgcgcc gcagcccaca gtccccaccg cgctcgaaag
      781 gaccagcgtc gtgaccagcg gcccgtagaa cccgggcccg tagccaagct ccctgtacca
      841 ggtgggcgtg aaggtctgga agacagacac ggtccagctg cgcgacatca tcacgccgat
      901 caccgcagcg atggcgagca tcggcacgac ctgtctgtac gccggcgcgg tcttgcgttc
      961 ggtcgcgctg ctgggtgccg ccgtgcgcat cctccacagg aggtatgtgc cggtggcgat
     1021 gcccgggatc accagccagc cggtgccgtg cagcccgaac catgtgaaga ggccgatgcc
     1081 gatcagcgga ccgatggcga caccgacggt gcccgcggtc acgtagatcg acatgccggc
     1141 gctgcggctt cgcttgggca gcagcgcgcg cacgtcgagc gcgccgaacg ggtggaaggc
     1201 cccggagccg agccccgacg cgcacgccag agccaccagc agcgggaacg tcggcacgaa
     1261 gccgaccagc gcgaaagtga tcgcggtcca gacgagcgcg acgcccgtga agcgggtccc
     1321 gtagcggtcg gcgatgactc cgaacagcgg ctgcgagatc gcggcggtgc cgctgtacgc
     1381 gaggctgatc aaaccgaccg ttgcgaagtt caggtcgaac cgtccgatca gcaccggata
     1441 aaggacggga atgacgccga cgtagctgtc gaccgtgagg tggccgagca tcagcgtgat
     1501 gagcttcccg tcgcgcagga agtccctcac gcgatctagg ttagcgggct aatcccacac
     1561 gctcttgcgc ttcgatcccg cggcccggga tgaccctacc cgcgataccg atcgatcgct
     1621 tgatgtatcg ctgcggtgcc gaactccagg gcgctcaccg gcacccgctc atcagtgtca
     1681 tgcatggtcc gctcaaggac cccaggatca cgacgccgtg gcggcgcgta gccccatgcc
     1741 gggtcgcctg agggcagaaa ccagcagggc cacgctggtg accagcggcc atgcgggaat
     1801 gctcgcgaac cagacatagc cgaacgcgac cagcatcggg ctggccgata ccccggcgag
     1861 tccgaggacg atggcgatga cgtgaattcc cgcgaccccc aacgtgaacc acgccagcca
     1921 tatggggacc gctcggctga tcagggctga aacaccgaca gcgaccagaa agaccgcgaa
     1981 cggaaccagg ctcagtactg acaacaccga gcccagctgc gccatcgagc caccaaagtc
     2041 ttttgagcca cccgtctccg gcactacgag gaccgcgatc tgcattattt cgatcgcaaa
     2101 tttcacggcg atggcgactg ttgcaacttg gacccctgtg gccgtgcttg gctcactgcg
     2161 tctgaccagc gtcgccacgt aaaggctgaa gaccatgtac aggagatagg ccagcagcag
     2221 cagcgctgcg gcgtcacgcg cggccgactg gtgcgtcgga gcccatcgac cccaatcccc
     2281 gagcgaatcg ccatacatcg gaaggttggg cgaaaagacg acgagaaaga acaccgcgct
     2341 ccccacgagt ccggtcgccg gcattgccag ccgcgtccag cgagcgaggt cagcgccttg
     2401 ccttgtcaca gccatgtcaa gcctccgccc acatcgctgc gcccagcagc acgaagcaca
     2461 cgccccaggc ggcgatgacg tactcgaagt cgtgacccgg cagggcgaac gaagggaaaa
     2521 ccacaagcac cacaaagaga ccggcgatca gcggtatgaa acggtgccaa cctttccaca
     2581 ggcgcgttcg caaagtggcg atgccggcaa gaatcaatcc cgccggcagc cctacggtgg
     2641 cggcgctgaa cagagggacg gcgacgtcgg tgctgaccac atcgacaaat tcggcgacgg
     2701 caagaatcgc ccagctgatc acggtgatcc aggtcccggc ccgtgcgata cggccgcggc
     2761 ccatcgctcc ggacgtccac agtcccagca tgccggccag cagaagcagg tggctgaggc
     2821 tgctcaggcc ggctgtcaac catgtcgtgc ccgggatcgc ggcgcccgcg acctgcagca
     2881 ggcccccggc catcgtcccc cacgccaacc agacaaccgt cgggatggta gttcgcggcg
     2941 cagtggcagt ggcctggttc attctgggat gttccctgag cgcgagccca tggtgaacgg
     3001 tgatgagatg ccctcgtagt tgacggtcat gctcatcccg ccggccgcgt gtatcagcac
     3061 gtggcagtgc agcatccaga tgcccgggtt gtcggccacg aagccaacgt cccagctgtc
     3121 gcccggtccg acgaggatcg cgtcgaggtg caccgggctg cctgtgatcg tccggccgtt
     3181 cttggccaga accgagaaga cgtggccgtg gatgtgaatc gggtgtgatt gccggctgtg
     3241 gttgacgatg tgcagccgga ccaggtctcc caatcggacg ttgatcggct gaatggacgg
     3301 tgcggcctgc ccgctgaagg tgtcggcgaa atcgaaggtt
//
LOCUS       DATGEM010000305         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1146780, whole genome shotgun sequence.
ACCESSION   DATGEM010000305 DATGEM010000000
VERSION     DATGEM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146780"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /gene="parC"
                     /locus_tag="VKQ27_09685"
     CDS             complement(<1..1450)
                     /gene="parC"
                     /locus_tag="VKQ27_09685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HLY89245.1"
                     /translation="MPDDLIGRIEDTRLSDALSERYLVYALSTIMSRSLPDVRDGLKP
                     VHRRLIYAMHLLRLDPTAGFKKCARVVGDVIGKFHPHGELAVYDALVRLAQEFAARFP
                     LVEGQGNFGNIDGDNAAAMRYTEARLTEVARAMLAGIDEDTVDFRQTYDGEESEPVVL
                     PGAFPNLLANGAAGIAVGMATSIPPHNAGEVCAAAIHLIRHPGATTADLLVHMPGPDF
                     PTGGVLVEDKTSILSAYETGRGGFRLRAKWHVEKGKHGTWQIIVTEMPYQVQKGKLIE
                     QIAQLMEERKLPLLGDIHDESTEIVRLVIEPKTRGVEPEVLMETLFRASGLETRFPLN
                     MNVLDATRTPLVMSLPQVLRAWLDHRHEVLVRRTRHRLAAIERRIEVLDGYLLVYLNV
                     DEVIAIIRHEDEPKPRLMARFKLSDTQAESILNMRLRSLRRLEEMEIRKEHKSLSKER
                     KDIQALLKDEKLRWARIVEELEQTRQKFGLGAL"
     gene            complement(1440..2189)
                     /gene="recO"
                     /locus_tag="VKQ27_09690"
     CDS             complement(1440..2189)
                     /gene="recO"
                     /locus_tag="VKQ27_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLY89246.1"
                     /translation="MEWDAAAIVLDARPYGEGDAIATVMTAEHGTHRGLARGGATRGR
                     AAVWQPGNLMQVRWVARLADQLGSFSAELVHPAAALAMEDALGLAMLSAACAVVEGAL
                     PEREPHPRVFEGLVHLIAHLSEGAALLPDLIRWETLLLADLGYGLDLTRCAVTGETAG
                     LAFVSPKTGRAVTAEAAGAWTSRLLRLPSFLVGGNVARPEDWRAGLRLTGHFLARDAF
                     GHHHRPLPAARQMLYDRVAALAAESEQQDAG"
     gene            2318..>3418
                     /locus_tag="VKQ27_09695"
     CDS             2318..>3418
                     /locus_tag="VKQ27_09695"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLY89247.1"
                     /translation="MITAFRRYLETWVVRAFFLIMVASFVVWGVGDVVRMIGANPTWV
                     AKVGDQTIEGQQFQDAYQRDMAQVTRRLPSGQEPSQDMRNSAAKQTLQQLLTEAALDQ
                     ELQRLHVVAPDAAVRQAVFATPAFRGANGQFDRQTFETVLRNNGLSEPRFLDMVRGQL
                     QQQQLLEAVTAGAAASESMLHPLYESQFEKRSADMVEFPLAAAPEPPVPVEAELRRWY
                     DNHPDQYSAPEYRRIKAIVLSPETLAKEIPITDADLHAAYQQHLAEYIKPEKRSAEVI
                     SVSDEAKANALAATWESGADWAAMQKAAKDAGASAISFDDATQSQFPDPDLAKDVFAA
                     TKDTVTGPTKGALGWHVVKVSAIDPGSTRSFDE"
BASE COUNT          592 a         1299 c          985 g          542 t
ORIGIN      
        1 ccagcgcccc caacccgaat ttctggcgcg tctgctccag ctcctcgacg atacgcgccc
       61 accgcagctt ttcgtccttc agcagcgcct ggatgtcctt gcgctccttc gacagcgact
      121 tgtgctcctt gcggatctcc atctcctcca gccgccgcaa cgaccgcagc cgcatgttca
      181 ggatcgactc ggcctgcgta tcgctcagct tgaaccgcgc catcagccga ggcttcggtt
      241 cgtcctcatg ccggatgatc gcgatcacct catctacatt cagatagacg agcagatagc
      301 catcgagcac ctcgatccgc cgctcgatcg ccgccagccg gtgccgcgtg cgccgcacca
      361 gcacctcgtg gcgatgatcg agccatgccc gcagcacctg cggcagcgac atcaccagcg
      421 gcgtgcgcgt cgcatccagc acgttcatgt tcagcggaaa ccgcgtctcc agccccgaag
      481 cgcggaacag cgtctccatc agcacttccg gctcaacgcc gcgcgtcttc ggctcgatca
      541 ccaggcgcac gatctcggtg ctttcgtcat ggatatcgcc gagcagaggc aacttccgtt
      601 cctccatcaa ctgcgcaatc tgctcgatca gcttcccctt ctgcacctga tacggcatct
      661 cggtgacgat gatctgccag gtgccgtgct tgcccttctc gacatgccac ttcgcgcgca
      721 gccggaaccc gccgcgtccc gtttcgtacg cggacaggat cgatgtcttg tcctccacca
      781 gcacgccgcc cgtcggaaag tcgggccccg gcatgtgcac cagcagatcc gccgtcgtcg
      841 cccccggatg ccggatcagg tggatcgccg ccgcgcacac ctcgcccgca ttgtgcggcg
      901 ggatcgacgt cgccatcccg acagcaatcc ccgccgcgcc attcgccagc aggttcggga
      961 acgcgcccgg caacaccacc ggctcgcttt cctcgccatc atatgtttgc cggaaatcga
     1021 ccgtgtcctc gtcgatgcct gccagcatcg cgcgcgcaac ctcggtcagc cgcgcctcgg
     1081 tgtagcgcat cgccgccgcg ttatcgccgt cgatattgcc gaaattcccc tgcccctcca
     1141 ccagcgggaa ccgcgccgcg aactcctgcg ccagccgcac cagcgcatcg tacacagcca
     1201 gctcgccatg cggatggaac ttgccgatca catcgcccac aacgcgcgcg cacttcttga
     1261 accccgccgt cggatccagc cgcagcaggt gcatggcgta gatcagccgc cggtgcaccg
     1321 gcttcagccc gtcgcgcaca tccggcagcg accgcgacat gatggtggac agcgcataga
     1381 cgaggtaccg ctccgacagc gcgtcagaga ggcgcgtatc ctcgatgcgg ccaatcaggt
     1441 catccggcat cctgctgctc cgattcggcg gccagtgccg cgacgcggtc gtatagcatc
     1501 tgccgcgctg ctggcagcgg gcggtggtga tggccgaacg cgtcgcgcgc caggaagtga
     1561 ccggtcagcc gcagcccggc ccgccagtcc tccggcctcg ccacattgcc tcccacaagg
     1621 aaggacggca gccgcagcag ccgcgatgtc cacgcccccg ccgcctctgc cgtcaccgcc
     1681 cgccccgtct tcggcgatac aaatgccagc cccgccgtct cgccggtcac cgcgcagcgc
     1741 gtcaggtcga gcccgtaccc cagatcggcc agcagcaatg tctcccaccg gatcaggtcg
     1801 ggcagcagcg ccgccccctc cgacagatgc gcgatcagat gcaccagccc ctcgaacacg
     1861 cgcggatgcg gctcgcgttc cggcagcgcc ccctccacca ctgcgcatgc cgccgacagc
     1921 atcgccaggc ccagcgcatc ctccatcgcc agtgccgccg ccgggtgcac cagttcggcg
     1981 ctgaagctgc cgagctggtc cgccagccgc gccacccagc gcacctgcat caggttccct
     2041 ggctgccaga ccgccgcgcg cccgcgcgtt gcgccgccac gcgccagccc ccgatgcgtg
     2101 ccgtgttcag cggtcatgac cgtggcgatc gcatcaccct cgccataggg acgggcatcc
     2161 aggacgatcg ctgctgcgtc ccactccatg gccgcgaccc gctagcaggt ttcgccgcgc
     2221 gggcctactg cccaccccag actccacggc gcgcgacgct ttcccgcccc gacgaactta
     2281 cctataccct gcgcctctcc gcttcagagg cgcctgcatg atcacagcct tccgccgcta
     2341 cctcgagaca tgggtggtcc gggccttctt cctgatcatg gtcgcctcat tcgttgtctg
     2401 gggcgtcggc gacgtcgtcc gcatgatcgg cgccaaccca acctgggtcg ccaaggtggg
     2461 cgaccagacc atcgagggcc agcaattcca ggacgcctac caacgggata tggcacaggt
     2521 cacgcgccgg ctgccctcgg gccaggaacc gtcccaggac atgcgcaaca gcgccgcgaa
     2581 acagacgctg cagcagctgc tcaccgaggc ggcgctggat caggaattgc aacgcctgca
     2641 cgtcgtcgcc cccgatgcgg cggtacgcca agccgtgttc gccacaccgg ccttccgcgg
     2701 tgccaacgga caattcgacc ggcagacctt cgagaccgtg ctgcgcaaca atggcctgag
     2761 cgaaccccgc ttcctcgaca tggtgcgcgg ccagttgcag cagcagcaat tgctcgaggc
     2821 ggtcaccgcc ggggcggcag catccgaaag catgttgcac ccgctttacg agagccagtt
     2881 cgagaagcgc tccgcagaca tggtcgaatt cccgctggct gccgcgccag aaccgccagt
     2941 gccggtggag gcggagctgc gacgctggta cgacaaccac ccggatcagt actccgcacc
     3001 cgagtatcgc cgcatcaagg ccatcgtgct gtcgcccgag accctcgcta aggaaatccc
     3061 gatcaccgac gccgatttgc acgctgccta tcagcagcat ctggcggagt acataaagcc
     3121 cgagaagcgt tctgcggaag tgatctccgt gtccgacgag gccaaggcca acgcactggc
     3181 cgcgacctgg gagagtggcg ccgactgggc agcgatgcag aaggcagcca aggacgccgg
     3241 cgcgtcggcg atttcctttg atgacgccac ccagagccaa ttccctgacc cggaccttgc
     3301 caaggacgtg ttcgcggcga ccaaggacac tgtgaccgga ccaaccaagg gcgcattggg
     3361 ctggcacgtg gtcaaggtct ccgccatcga tccgggcagc acgcgcagct tcgatgag
//
LOCUS       DATGEM010000306        23860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_511684, whole genome shotgun sequence.
ACCESSION   DATGEM010000306 DATGEM010000000
VERSION     DATGEM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 23860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23860
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511684"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VKQ27_09700"
     CDS             complement(<1..765)
                     /locus_tag="VKQ27_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HLY89248.1"
                     /translation="MDLDDDACYRALTTRDARFDGRLFVAVRTTGIYCRPICPARTPK
                     RENVTFYPTASAAQEAGYRPCLRCRPESSPDLAAWRGTSNTVSRALALIGEGALDNEA
                     VDTLAERLGVGERQLRRLFKQHLGASPIAVAQTRRVLFAKQLIVETQMPFAEVALASG
                     FGSIRRFNATFHALYDRPPRELRRRSMRDVDRSAAAGITLNLPYAPPYDWDAMIGFLA
                     AREIAGIECVDGVMRYQRTVVLDGVQGTVSVEPNDRG"
     gene            complement(843..1340)
                     /locus_tag="VKQ27_09705"
     CDS             complement(843..1340)
                     /locus_tag="VKQ27_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HLY89249.1"
                     /translation="MEWAEETIIRLRALWDEGLSTAEIGRRLGVSKNAVVGKAHRLDL
                     PARPSPIRRDGSGSSAPRRSTPRRVAGPTLPPLSSTAAIVGSAVMGPPVATLVPKPAP
                     APRVVPPAPRPYGRVVTCCWPIGEPGTRSFRFCDDASEPGKPYCTEHAKLAYVKVRDR
                     REDAA"
     gene            complement(1476..1718)
                     /locus_tag="VKQ27_09710"
     CDS             complement(1476..1718)
                     /locus_tag="VKQ27_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLY89250.1"
                     /translation="MGSFSIWHWMVVLLVVLLLFGGGKVSSLMGDFAKGIKAFKKNMA
                     EGEDASMEASGEKPTGSISGPAGEAAPRQRETTAAH"
     gene            2113..2322
                     /locus_tag="VKQ27_09715"
     CDS             2113..2322
                     /locus_tag="VKQ27_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLY89251.1"
                     /translation="MPKGTVKWFNPTKGYGFIAPDTGGKDVFVHISAVQKAGLRSLSE
                     GQKIGFEVEQQQNGRTAAVNLSQSD"
     gene            complement(2461..3033)
                     /locus_tag="VKQ27_09720"
     CDS             complement(2461..3033)
                     /locus_tag="VKQ27_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89252.1"
                     /translation="MALSSTAIRIILDRIEATIDQKILSQSVATKSKRIGDPQQDEFW
                     GQLVLYEQDLRRILRQLVGRQIALENERSLLWRIPRQYRYSARQSVNDRESATLDLVE
                     QAEAILRKLMELSGDAATMKPADWEAIGEKAMELADKLDHALVHTVVQQVQKGPAFTS
                     TALPGLGLDHLAPLVGLLIAYIMSKRKRRN"
     gene            3157..3798
                     /locus_tag="VKQ27_09725"
     CDS             3157..3798
                     /locus_tag="VKQ27_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLY89253.1"
                     /translation="MTSSATLFLRQFMRSPTTIGAILPSSPALAEAMLAPIDFGTART
                     IVEFGPGTGAFTSEIAKRLRPGCRYLGIELNPVFARNLTARFPRLCFVNGSAADLTPI
                     LAERDIDDVDAIVCGLPWATLPVSLQEAVFAAMDRALVPGGVFVTFGYFQSLVMPAAQ
                     ALRRRLLREFPDVRRSPVVWANVPPAFAYICRKREQAFLAPGGHPVAGRLQEI"
     gene            3849..4511
                     /locus_tag="VKQ27_09730"
     CDS             3849..4511
                     /locus_tag="VKQ27_09730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY89254.1"
                     /translation="MSDTLSRRQAGRPHARMDVEAVRAAYRRWAGIYDTVFGSILLGA
                     RRRAVALVNQLPGREVLEVGVGTGLALPYYNADRRITGIDLSAEMLAVAGRRVAETRL
                     RNVAALREMDAEATDFPDASFDTAVAMFVASVVPHPKQLLAEMRRVVRPGGNILFVTH
                     IAADKGPRWWVERTLAPASRALGWHPDFSLEAMCSAEEIATCFCTPVPPFGFFTLVRL
                     GN"
     gene            4728..5603
                     /gene="coxB"
                     /locus_tag="VKQ27_09735"
     CDS             4728..5603
                     /gene="coxB"
                     /locus_tag="VKQ27_09735"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLY89255.1"
                     /translation="MVRRISNYRRLFAALLTFMALPGVAFAQAVGAPKPWEIGMQAAY
                     GPLKRDEIWLHNIVLVIITLITLFVAGLLVWVIYRYNARRNPVPSRTSHNTLVEVLWT
                     VIPVVILVGMAIPSFRLVYYEDRTHDPELTIKVTGHQWYWEYTYPDNNNIDFSSYIIP
                     DDQLKPGQMRLLTVDNELVIPVNKNIRILTTSGDVIHSFFIPALGVQRYAIPGRTIET
                     WMRADTAGTFYGECNQICGTNHSRMPIVVRAVPEQEFNAWLAQAKTKFASETPQSPDT
                     TNEPRQQVASAGVQH"
     gene            5603..7195
                     /gene="ctaD"
                     /locus_tag="VKQ27_09740"
     CDS             5603..7195
                     /gene="ctaD"
                     /locus_tag="VKQ27_09740"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HLY89256.1"
                     /translation="MSATTHDHAHDHHDHKPGFFVRWFCSTNHKDIGTLYLTFAVVGG
                     LMGGILSEIMRAQLSFPGNHVVTDGQEWNTIITSHGLLMIFFSVMPALIGGFGNWFIP
                     LMIGAPDMAFPRLNNISFWLLPPAFTLIMIGLFMGESGSGWTLYPPLSNQTYETGMGM
                     DCTLFALHLAGASSLLGAINFITTIFNMRAPGMTLHKMPLFIWAMLVTAFLLLLSIPV
                     LAGAITMLICDRNFGTSFFDPAGGGDPVLFQHLFWFFGHPEVYIMIVPGFGIISHVIS
                     TFSRKPVFGYLGMAYAMVAIGVIGFVVWAHHMFISGIDVDTRAYFMAATLVIAVPTGI
                     KIFSWIATMWGGSIELKTPMLWAIGFLFVFTIGGVTGVVLANAGIDEIVHNTYYVVAH
                     FHYVLSLGAVFSIFAGFYYWIGKMSGHQYPEFWGKVHFWLTFIGVNLTFFPQHFLGLS
                     GMPRRYPDYPAAFAGWNFVSSMGAYVSGVAFLVFLYVVFRTFTSKEHVADNYWGDGAT
                     TLEWTVSSPPPFHTFQELPRFR"
     gene            7342..8259
                     /locus_tag="VKQ27_09745"
     CDS             7342..8259
                     /locus_tag="VKQ27_09745"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HLY89257.1"
                     /translation="MSEAVTTTTTEAAELSDWFALLKPRVMTLVVFTGLIGLLIAPGH
                     LNPVLAFTAVLCIAVAAGACGAINMWYDRDIDAVMRRTRNRPIPAGRIEPGAALGFGV
                     TLAVGAVIMMGLAVNIAAASVLALSIAFYVFVYTMWLKRRTPQNIVIGGAAGAFPPVI
                     GWAAVTGSVDLVPLVLFAIVFFWTPPHFWSLALFANSDYQRAGVPMLPVVAGAKVTRR
                     HIVAYTLLLVPLSLTPWLIGFAGPVYGISALMLGLGFIASVFRVASDKQDAAGVSLTN
                     DAPARMAFKFSIIYLFVLFAALAVDRLVG"
     gene            8256..8393
                     /locus_tag="VKQ27_09750"
     CDS             8256..8393
                     /locus_tag="VKQ27_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89258.1"
                     /translation="MTLAVALDPDDMRRRRARNWAVLIVLLALSALFYAITIVKMTHH
                     G"
     gene            8523..9116
                     /locus_tag="VKQ27_09755"
     CDS             8523..9116
                     /locus_tag="VKQ27_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLY89259.1"
                     /translation="MRTSNRAIGISLATVIVGMAGVSFAAIPLYRVFCSVTGYDGTPQ
                     IGAAAAPGATSQQITVRFNANTHPSLPWDFHPQQPELRVALGDEHLAFYTARNTASAP
                     LTGVALYNVTPDTVGKYFHKTACFCFNQQTLAAGQQMQFPLSFWVDPAIAKDPNTADI
                     HTITLSYTFFRTLEDAQKSGALAEAGPHVGPLQTQTP"
     gene            9171..10022
                     /locus_tag="VKQ27_09760"
     CDS             9171..10022
                     /locus_tag="VKQ27_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLY89260.1"
                     /translation="MSEHIHGVTTIESSGLKQPYHLVDPSPWPIVGGLAGFCVVFGII
                     LAAHFGSYALLVIGALLVFVTMFFWWRDVIRESRTPGLHTPVVRLGLRYGMLFFITSE
                     VMFFVGFFWAFFNFALLPETQGNGQTVWPPTTIHTFDPFHLPFLNTMILLLSGTTITW
                     AHHALLNNDRKGLTAGLGLTILLGLSFTMCQAIEYSDAPFKFVGGGVYPSTFFLATGF
                     HGFHVMVGTCFLIVCWFRARAGQFTPERHFGFEAAAWYWHFVDVVWLFLFTCIYWWGA
                     SPVTAPH"
     gene            10019..10378
                     /locus_tag="VKQ27_09765"
     CDS             10019..10378
                     /locus_tag="VKQ27_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HLY89261.1"
                     /translation="MTEPLPSPLRVALGCRCPRCGKGKLYKGLLEIRDRCDVCGLDLR
                     KNDTGDGAAAGVTLVLGAIVVALAFWVEFRFEPPFWVHIMLWPLITVLGAIWLTRVAK
                     AALVAMQFRHRSSEMGL"
     gene            10375..11067
                     /locus_tag="VKQ27_09770"
     CDS             10375..11067
                     /locus_tag="VKQ27_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HLY89262.1"
                     /translation="MTGKLRRLLWPGVMTVFMLAVLLGLGTWQVQRLHWKLGILAQIA
                     RAEAGPPIPLPAVPTQFAKVAVAGHLRDDLAATFGADVRDTPGGPVLGTQLIVPLERP
                     GAEPILVDRGWIPGKPPHPIPPVGGDVTLQGYVHPGYTPGWFSAADNPGARQFYTLDP
                     AAIGAALGLPRVAPFVLIELGSAPPGGYPLPAQHLPQPPNNHLSYAITWYGLAVALVV
                     IFVIWARKVLAE"
     gene            11064..12551
                     /locus_tag="VKQ27_09775"
     CDS             11064..12551
                     /locus_tag="VKQ27_09775"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HLY89263.1"
                     /translation="MTAYDRLAARFARIATLGEASSVLNWDAATMMPPGGGAARGDQL
                     AVLAGMSHALLVAAEVGDDLAEAEQAGENDPWRTANLRLMRHAYTRATAMPADLVEAQ
                     ARANSACEKVWREARRQSNFAMVRPHLEEVVRLVREAAAALAPALGLSAYDALADGYQ
                     RGMRAAEVAPVFASYEAFLATALPRAEELQAGRPVPVRPHGPFPITRQEALCRELSER
                     LGLDFAHARLDRSAHPFSGGTVTDVRITTRYDEADFTQAVLAVVHETGHALYERGLPG
                     DYARQPVGEAAGMAAHESQSLIVEMQACRSDAFLSYFGPKLHAAFGNDTTPYQPENLA
                     RLWRRVERSYIRVDADEMTYPAHVILRFRLEQALIDGALAVADLPGAWNEAFQRLLGM
                     TPPDDARGCLQDIHWYDGALGYFPSYTLGAMAAAQLMAAARRAVPSLDASLARGDLSP
                     LLHWLRANVHGKGSLLEFNPLLQAATGEPLNPAYFQQHLTARYLQ"
     gene            12593..12982
                     /locus_tag="VKQ27_09780"
     CDS             12593..12982
                     /locus_tag="VKQ27_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89264.1"
                     /translation="MQIQRAAVVAAFCAVLSACASKPPPAPEPKPPPPPEIDGEYRGT
                     TTRFQADTKACPHPGLVDFQVIQHQFQYRWDYDTFVLATIAPDGGVTGGAERITLVGK
                     QNGPIIEGDVTNGNCGLHFTVKLHPAP"
     gene            13019..14416
                     /gene="thrC"
                     /locus_tag="VKQ27_09785"
     CDS             13019..14416
                     /gene="thrC"
                     /locus_tag="VKQ27_09785"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLY89265.1"
                     /translation="MQYVSTRGTAPVRDFAGVLLAGLAEDGGLYVPETWPVFTPADWR
                     ALRGLPYHALAARVIQPFVGKAIPAATLAALCREAYAGFGHPAVVPLVQLETGLFVQE
                     LFHGPTLSFKDMALQLIGRLFDHVLTERDTRVTIVGATSGDTGSAAIEACAGRDRVDI
                     AFLHPHHRTSEVQRRQMTSVTAPNVANIAIDGTFDDCQDLVKAMFADTPFRQEMHLSA
                     MNSINWARIAAQIPYYLASALALGAPDREVAFSVPTGNFGNILAAWVARRMGLPIARL
                     IVGSNRNDILARFLTSNDMSITSVEPSLSPSMDIQVSSNFERLLFELLERDAQATAAA
                     MEAFRRTGRMTVPDAQWHRCRTVFHGFRLDDAGTEAEIARVYQATGYLADPHTAIGIA
                     AARANPVHGVPIVTMATAHPAKFPDVIARATGIHPPLPPRLTDLYERPEQFSVLPNDL
                     AAVEAKVRALAHRNA"
     gene            14430..15581
                     /locus_tag="VKQ27_09790"
     CDS             14430..15581
                     /locus_tag="VKQ27_09790"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY89266.1"
                     /translation="MSQSLEGVRVLELARYQAGPRGGMILSDLGAEVIKIERMGGEET
                     RHHAPMVRGQSIYFTVYNRGKKSICLDMRQERGKEIFTGLVKTADVVLENFRPGTMKA
                     MGFDYERLRAINPRIILVSVSGFGQYGPYTDRPAFDPLGQAMSGLMSLTGKPVGQPLG
                     TASSVVDRYTALHATIGTLAALRHRDRTGEGQLVDCCLLDSALTMVEIPTSYYLSTGE
                     EGGEGGRPPYRAKDGYVVISAGGREMAARLAQLVSSGTIAMSGEPITAGQGGSDTRRA
                     LEAWCAERPVEEICSALMEIGVPVAPVRTIPEVAKDPHLWEREMLVKMEDAVAGELYV
                     PGVTVKMSKTPGRVGPVPTPGQHTDELLGQLLGYDADTLGALRRDAIIA"
     gene            15638..16159
                     /locus_tag="VKQ27_09795"
     CDS             15638..16159
                     /locus_tag="VKQ27_09795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLY89267.1"
                     /translation="MALTVATGQEIDGKAFWTTLGQRATGMTVVTADGDDGPTGFLGL
                     SAAHVTASPPTMLVSVDRKTSALAGILARRHFAVNFLPADAGQVADAFGGRAGVSGAD
                     RFAPGAWRTLATGAPVYNDALGAFDCTVAQVVQHGDISIIIGTVVAAAWRPDGEPLVF
                     FRGKSFTGMGGAA"
     gene            complement(16201..17433)
                     /locus_tag="VKQ27_09800"
     CDS             complement(16201..17433)
                     /locus_tag="VKQ27_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLY89268.1"
                     /translation="MLSDERNRLLCEVGPGTKMGALLRRYWQPIAAVSELDDAPTKAV
                     RLLGEDLVLYKDLSGHYGLLERHCPHRSADLSYGIVEDCGLRCNYHGWMFASDGACLE
                     QPFEDIDAPQIRFREQIRIKAYRVEAKAGLLWAYLGPEPAPLVPNWEPFTWDNGFVQI
                     VFATVPCNWFQCQENSIDPVHFEWMHDNWGARLKGRNDYAPRHLKLAFDEFEYGITYR
                     RVREDSDEKNPLWTVGRVCLWPNALFTGNHFEWRVPIDDTNTLSVGWFFTRVPRDREP
                     YVQNSIPYWYSPITDEKSGRWITSHIMNQDFIGWVGQGAITDRKREHLGRSDRGIIMM
                     RQRFFRDLEALGSGDDPKAVVRDAEVNRCIELPIIGRKSFVEGLERDEIVRREFPFLV
                     GQPDAVRRAYNDAMGFAD"
     gene            complement(17449..18534)
                     /locus_tag="VKQ27_09805"
     CDS             complement(17449..18534)
                     /locus_tag="VKQ27_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89269.1"
                     /translation="MSDEPDSSDKVALELRKLRWELSFWARAAAVAVPIAAFVVAAAQ
                     TYIAVQDSKSKDLLALRDLELKRIESGEKLVEFTTAQTELLLSADIEKRTQALTYAST
                     LLPPQQACTVINAVIIASPGHADFIAPAQHIRALIVKQHPELLALTECTDDAIFDTQN
                     EISIAVAQAKQESQSPEYLPAVAPVAAPPPPAPAPVPTAVPSPVPTAPAPAPQVKAAA
                     APKAVPTAPPPTAAAAPLCARPGDTATLPLVVYYQIVQANDRDSAARIGFEMPAEFPS
                     AGIELVAATAPARQTPQVRYYRAEQKSAAEWLACMLTEAYAKVTGSKPRVNGTDANFA
                     VVPLAGRYKGLPAHRAEVWFPAITQSN"
     gene            complement(18565..19521)
                     /locus_tag="VKQ27_09810"
     CDS             complement(18565..19521)
                     /locus_tag="VKQ27_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HLY89270.1"
                     /translation="MAIIVGSGEHRYEVIENWGKLPDGWRYGEVAAVGVDSKDNVYVF
                     ARSEHPMMVFDGEGNFLRSWGEGLFKRPHGVHLAPDDTIYCTDDGDHTVRRLTLEGKL
                     LLEIGVPGEPAPMYGGDPFNRCTHTALSPEGDLYVADGYGNARVHKYSPDGKLQFSWG
                     EPGTDPGAFNIVHNICCDADGWVYVADRENHRVQVFDGKGKYQSQWNNLHRPCGLCMT
                     QGRNPIAYIGESGPGMAVNAKSPGIGPRVSIVTHEGKPIARLGDATRERSSQFIAPHG
                     VAIDSRGNIYVGEVSRTAMRNRGTPIAPEEDIRCMQKLVKLS"
     gene            19600..20592
                     /locus_tag="VKQ27_09815"
     CDS             19600..20592
                     /locus_tag="VKQ27_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY89271.1"
                     /translation="MTDTHYASYTFNDFRLQNGEVMPEITLAYCTRGTLAPDGRNAVL
                     VTHGYTSSHRMISSGTGSSEGAWSTLVGPGAPIDTGRDFVVCSNMLGSSYGSTNAASR
                     DPRTGRPYGSRFPNITLPDIVTAQRHLLDHLGVKHLRAVVGPSYGGFQAFQWAVTFPD
                     FMDGIVPVVTSPRAQSTDRVATLRARFERNPNWHGGDYYDTPGMFDSLLELRIETLKR
                     YGMPGSDEEITRVASVWAREFDANSLVILGRAMEAYDVTPEMRRIRVPMLYVLSRTDA
                     LFPPGLAPGVMASLRDAGVEASYFEIDSELGHTASGTDAAKWASALRGFMARLP"
     gene            complement(20594..21832)
                     /locus_tag="VKQ27_09820"
     CDS             complement(20594..21832)
                     /locus_tag="VKQ27_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY89272.1"
                     /translation="MTHITRRAAFHIGASAALLTLGTRRGYAADKSVTIGIDLSLTGA
                     DAETAVRIRDGFMMAIDEANEKGGPAGYHLNVLLLDDGTATAGQYDPAQGATNARKMV
                     ADPSVVAALGPMSSTPGKAMSPIFSQGNLATITPTSTNPDITDPKFAALYRPAGKAVY
                     FRTVTTDAYQGPNMANYFADTLKVKSVYGLDDSGAYGIGISTAFETQAKKRGIQVLGH
                     DRLDPKAADYTPALTKIKALGAQAIYYGGDAQAGVKLAKQAYDLIPNAIKAGGDGMYG
                     PSILQGAGFPSADGWYATVASPHMMEDTELQPWVKRFTAKTGRQPSDYSITAYDAAMV
                     VINAVERVAKSGKPVERVAVRDAIASTKLKTLQGEVSFDDNGDIRQRVVAVFQVKHDA
                     AHPVDDLVHQYRYVGVAPEA"
     gene            complement(22019..23092)
                     /locus_tag="VKQ27_09825"
     CDS             complement(22019..23092)
                     /locus_tag="VKQ27_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLY89273.1"
                     /translation="MRDETRMLLESMGRLFEDHCTKAVADAAEAGTFPAALWQAVSDT
                     GVPLAGVAGSEVEWSDLYAALRVAGRHAAPIPLAETMLAAWLASSAGFTPGEGPMTVG
                     PVRAADKLTLQRDGNGWRLFGRASRLPWGQQAGQVALIATDPEGNTMVVFGDGIPGAT
                     PPSGGANMAGEPRDTYEFAGAGLSGNEVAPAPAGIDQAALYRRGALARAMMMAGAMER
                     ALDTAVGYAGERKQFGRPIGKFQAVQQNLAVLAGQVAAAGAAADAGVEALAVSDPAKQ
                     EFLVGIAKTRVGDAATIASEIAHQVHGAIGFTKEYSLQLSTRRLWSWRDEFGGDTEWA
                     AKVGAYVCSGGADALWPTLTNDS"
     gene            complement(23107..>23860)
                     /locus_tag="VKQ27_09830"
     CDS             complement(23107..>23860)
                     /locus_tag="VKQ27_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89274.1"
                     /translation="SGSDLAGTKTRAVKVDGGFRINGSKLWTSNAHRADYMILFCKMA
                     SDGQEEVDRHAGATQFLVDLKNSPGIEIRPVINLLGEHHFNQIFLTDLFVPDNRLFGE
                     IGNGWRQVSSELAFERSGPDRFLVLFKLLTEMVRVAGPNPDRATAAALGRLVAQLATL
                     RRMSLSIAGMLQDGLQPLNEAAVTKDLGTRYEQDLPVVARQLFPDEPDLQAVDTYVSM
                     LARGIGNAPRLSIQGGTREILRGIIARGLGLR"
BASE COUNT         4016 a         7667 c         7672 g         4505 t
ORIGIN      
        1 gcctcgatca ttcggttcaa cgctgaccgt tccctgcacg ccatccagca caaccgtgcg
       61 ctggtagcgc atcacaccat cgacgcactc gatcccggca atctcgcgcg ctgccaggaa
      121 gccaatcatc gcgtcccaat cgtaaggcgg cgcgtatggc aggttcagcg tgatgccagc
      181 ggctgcggag cgatccacat cgcgcatgct cctgcgccgc aactcacgcg gcggccggtc
      241 atatagcgcg tggaacgtcg cgttgaaccg ccggatactg ccaaaccccg atgccaacgc
      301 gacttcggca aacggcatct gcgtttcgac aatgagctgc ttcgcgaaca gcacgcgccg
      361 cgtctgtgca accgcaatcg gcgaagcacc gagatgctgc ttgaacagcc gccgcaattg
      421 ccgttcacca acacctagcc gctccgccag tgtatctacg gcctcattgt ccagcgcgcc
      481 ctcgccgatc agcgcgagcg cccgcgatac ggtattcgat gtcccgcgcc acgcggcgag
      541 gtccggcgaa ctctccggcc ggcagcgcag gcacggccga taaccggctt cctgcgcggc
      601 actcgccgtc ggatagaacg tgacgttttc ccgcttcggc gtgcgggccg ggcagattgg
      661 tcggcaataa atccccgtcg tgcgcaccgc gacgaacaga cggccgtcga agcgggcatc
      721 tcgggtggtc aaggcgcggt agcaggcgtc atcatccagg tccatgcgcg caccatcgca
      781 gatggcagcg cgccggtctc gccgttttcg gacgcgattg cgcgggcctg cttaacgagc
      841 cctcacgccg catcctcgcg acggtcgcgc accttcacgt aggccagctt ggcgtgctcg
      901 gtgcagtacg gcttgcctgg ctcagaggca tcgtcgcaga accggaagct gcgcgtgccc
      961 ggttcgccga tgggccagca acaggtgacc acacgcccgt agggccgtgg tgcgggcgga
     1021 accacgcgcg gcgctggcgc gggcttcggc actagcgtgg cgactggcgg acccatcacc
     1081 gcgctgccaa caatcgccgc cgtactggaa agtggcggca aggtcggccc cgccaccctg
     1141 cgtggtgtcg agcggcgcgg tgcgctgcta cccgagccat cgcgccggat cggcgacgga
     1201 cgggccggca ggtccagccg atgcgccttg cccactacag cgtttttcga aactccgagg
     1261 cgccgtccga tttcagcggt ggacagtccc tcatcccaaa gcgcgcgaag ccggataatc
     1321 gtttcttcgg cccactccat ctcgtggtcc ccccgatggt gcaaccacaa gatacggtat
     1381 acggcaaaac gccgccgtca actcatcatt ggcggcggac cacaaaaact tgtggaaaac
     1441 cgtgtatgca accgcaagaa cttgcgtgaa gggcgtcagt gcgcggcggt tgtctcgcgc
     1501 tggcgcggcg cagcctctcc cgccgggccg gatatcgagc ccgtcggctt ctcgccgctc
     1561 gcctccatcg aggcatcttc gccttccgcc atgttcttct tgaaggcctt gatgcctttc
     1621 gcgaagtcgc ccatcaggct gctgacctta ccgccgccga acagcagcag aacgaccagc
     1681 agcaccacca tccagtgcca aatgctgaag ctacccataa atcctccaag cgggctgatc
     1741 gcggcgcccc gttggcgcga aatcctcgcc ggcccaagcg aggcaatcta gcggcaccaa
     1801 cgcctgctgc caacgacatg gcgaaatcca cgcatggatg caagcggttt ggagtccagg
     1861 acggcaacgt ggccggtttg caatgcacgt gacaggattc ttgccaaatg attgctggac
     1921 tgattgcggc gacgacttgg tccgcgtatc gtgcctccgg caggatgata ccgttcggca
     1981 tcgaacaata acggatgggg atcttgctac agggaaggaa agcgtcccgg agcgaatccg
     2041 ttgcgctttc ctataatttg gtcgacgtct cctccggtgg gcgtcgcccg caggtagaaa
     2101 ggcaacgcgt cgatgcccaa gggcaccgtt aagtggttca acccaaccaa aggctatggc
     2161 ttcattgccc ccgacacagg aggcaaggat gtcttcgtgc atataagtgc cgtgcagaaa
     2221 gccggcctgc gcagcctgag cgagggccag aaaatcggct tcgaagtcga gcagcagcag
     2281 aacggccgca cagcggcagt caatctgtcg cagtccgact agccgtcata gcgggcggcg
     2341 gtctccgttt ggccgccacc gcgtcttaca ccaaccatgc gccgcccgcc gcggcgcgtg
     2401 gttactacgc gtggcaacct cctccgaaat tcggacagag gcccaacgtt gcgtgggtgc
     2461 tcagttgcgc cgcttccgct tgctcatgat gtacgcgatg agcagcccga ccagcggggc
     2521 aagatggtcg agccccaggc cggggagcgc ggtggaagtg aaggcggggc ccttctgcac
     2581 ctgctgaacc acagtgtgga ccagggcgtg atccagttta tcggccagct ccatcgcctt
     2641 ttcgccgatg gcctcccagt cggcgggctt catcgtcgcg gcgtcgccag agagttccat
     2701 cagcttgcgc aagatggctt ccgcctgctc caccagatcc agcgtggcgc tctcgcggtc
     2761 gttcaccgac tggcgcgcgg agtagcgata ctggcgcgga atgcgccata gcaggctccg
     2821 ttcgttctcg agcgcgatct ggcgcccgac cagctggcga aggatgcggc gcaggtcctg
     2881 ctcgtaaagg acgagctgcc cccagaactc gtcctgttgc ggatcgccaa tgcgcttgga
     2941 tttggtggcg acgctctgcg agaggatctt ctggtcgatc gtggcctcga tgcgatcgag
     3001 aataatgcgg atagcggtgg atgacagggc catacggtcg tcgctccgtc tgcggagacg
     3061 gcggcataac acgccacctg caacgacttt ccgcgcggtc cgatcacgtt tcaggtaggg
     3121 ttgccgagac cacgggtcgc ctcgcattgt ggggccatga caagctcagc gaccctcttc
     3181 ctgcgtcagt tcatgcgatc gccgaccacc attggtgcga tcctgccgag ttcaccggca
     3241 ttggccgagg cgatgctcgc cccgatcgac ttcggcaccg cgcgcaccat cgtggagttc
     3301 ggcccaggca ccggggcttt caccagcgag attgcgaagc gcctgcgacc cggctgccga
     3361 tacctgggga ttgagctgaa cccggtgttt gcccgcaacc tgaccgcccg ctttcccagg
     3421 ctgtgcttcg tgaacggcag tgcagccgat ctgacgccga tcctggcgga gcgcgacatt
     3481 gacgatgtcg acgcgatcgt ttgcggactg ccctgggcga cattgcctgt gtcactgcag
     3541 gaggcggtgt ttgcggcaat ggatcgcgca ttggtgccag gcggcgtgtt cgtcacattc
     3601 ggctatttcc agagccttgt gatgccggct gctcaggcac tgcgccgccg gctcctccgg
     3661 gaattcccgg atgtgcgccg ctcaccggtt gtttgggcga acgtgccgcc cgcgttcgca
     3721 tatatttgtc ggaagaggga acaggcattc ctggcaccgg gtgggcatcc ggtggcaggg
     3781 aggttacaag agatttaagc ggagcgattc gccacccgcc agcacggccc gggaggaaca
     3841 cgaccccgat gagcgacacc ctgagccggc gtcaagccgg acgcccgcac gcgcggatgg
     3901 atgtggaggc ggtgcgcgcc gcctaccgcc gctgggcggg aatttacgac acggtgttcg
     3961 gcagcatcct gctgggtgcg cggcgtcggg cagtggcgtt ggtgaaccag cttcctgggc
     4021 gcgaagtgct ggaggtgggg gtcgggaccg gcctcgccct gccgtattac aacgcggacc
     4081 gccggatcac cggcatcgat ctgtcggctg agatgctggc ggtggccgga cggcgggtcg
     4141 cggagacgcg actgcgcaac gtcgcggcgc tacgggagat ggacgccgaa gcgaccgact
     4201 ttcctgacgc atcgttcgac accgcggtgg cgatgttcgt ggcgtcggtg gtgccgcatc
     4261 caaagcagtt gctggcggaa atgcgccggg tagtgcgtcc cggtggcaac atcctgttcg
     4321 tcacgcatat cgctgccgac aaaggaccac ggtggtgggt cgaacggacg ctggcgccgg
     4381 cttcgcgcgc attggggtgg catccggact tttcgctgga ggcgatgtgt tcggcggagg
     4441 aaattgcgac ttgtttctgc acgccggtac cacccttcgg tttcttcacg ctggtgcggc
     4501 tcgggaactg atcgcgcgca accgccatgg ccgggcctga cccggccatc tgcttcggac
     4561 ggcaacggtg ttgtgtgggt ggaaggcgtc ggcccggtca agctcagcca tgaccagggc
     4621 gagagctgtt ccgcaacgct cactcccttg atctcctgca cgtggtccgg taggccacgc
     4681 attggagagc cggcccggca cgggaccgga aaggggaccg aagcgggatg gtgcggcgga
     4741 tttcgaatta ccggcggctg ttcgccgcgt tattgacgtt catggcgctg cctggcgtcg
     4801 catttgccca ggcggtgggt gctccaaagc cctgggaaat cggcatgcag gcggcttacg
     4861 ggccgctcaa gcgagacgag atctggctgc acaacatcgt gctggtcatc atcaccctga
     4921 tcacgctgtt cgtcgccggg ctgctggtct gggtgatcta tcgctacaat gcgagacgaa
     4981 acccggtgcc gagccggacc tcgcacaaca cgctggtcga ggtgctgtgg acggtgatcc
     5041 cggtggtgat cctggtcggc atggccatcc cgtcgttccg cttggtctac tacgaggacc
     5101 ggacgcacga cccggaactg accatcaaag tgacgggaca tcagtggtac tgggagtaca
     5161 cctacccgga caacaacaat atcgacttca gcagctacat catcccggac gatcagctca
     5221 agccgggcca gatgcggctg ctgacggtgg acaacgagct ggtgatcccg gtcaacaaga
     5281 acatccgcat cctgaccacg agcggcgacg tgatccacag cttcttcatc cccgcgcttg
     5341 gcgtgcagcg ctatgctatt ccgggacgga cgatcgagac ctggatgcgc gcggatactg
     5401 ccggcacgtt ctatggcgag tgcaaccaga tctgcggcac gaaccacagc cgcatgccga
     5461 ttgttgtgcg tgcggtgccg gagcaagaat tcaacgcctg gctggcgcag gcgaagacaa
     5521 aattcgcaag cgagacgccg caatcgcctg ataccacgaa cgagccgcgc cagcaggtgg
     5581 cgtcggcggg agtgcagcac tgatgtccgc gaccactcac gatcacgccc acgatcatca
     5641 cgaccacaag ccgggattct ttgtgcgttg gttctgcagc accaaccaca aggacatcgg
     5701 cacgctctac ctgaccttcg ccgtggtggg cgggctgatg ggtggaatcc tgtcggagat
     5761 catgcgggcc cagctcagct ttccgggcaa ccatgtcgtc accgacgggc aagagtggaa
     5821 caccatcatc acctcacacg ggctgctgat gatcttcttc tcggtcatgc ccgcgctgat
     5881 cggcggcttc ggcaactggt tcatccctct gatgatcggc gcaccggaca tggcgtttcc
     5941 ccggttgaac aacatcagct tctggctgct gccgccggcc ttcaccctga tcatgatcgg
     6001 gctgttcatg ggtgagtcgg gatcgggctg gacactgtat ccgccgctct cgaaccagac
     6061 ctacgagacc ggcatgggta tggattgcac gctgttcgcg ctgcaccttg ccggcgcgag
     6121 ctcgctgctg ggcgcgatca atttcatcac cacgatcttc aacatgcgcg cgcccggcat
     6181 gacgctgcac aagatgccgc tgttcatctg ggcaatgctg gtcacagcgt tcctgctgct
     6241 gctgtcgatc ccggtactcg ccggtgcgat cacaatgctg atctgcgacc gcaatttcgg
     6301 cacctcgttc ttcgatccgg caggcggcgg cgatccggtg cttttccagc acctgttctg
     6361 gttcttcggc catcccgagg tctacatcat gatcgtgccg ggcttcggca tcatcagcca
     6421 tgtgatctcg acgttcagca gaaagccggt gttcggctac ctgggcatgg cctacgcgat
     6481 ggtcgccatt ggggtgatcg gcttcgtggt gtgggcgcac cacatgttca tctccggcat
     6541 cgacgtcgat acgcgcgcct acttcatggc ggcgacgctg gtcatcgcag tgccgacggg
     6601 tatcaagatc ttctcgtgga tcgccacgat gtggggcggt tcaatcgagc tgaaaacgcc
     6661 gatgctgtgg gccatcggct tcctgttcgt cttcaccatc ggcggcgtca ccggagtggt
     6721 gctggcgaat gcgggcatcg atgagatcgt gcacaacacc tattacgtcg tggcgcactt
     6781 ccactacgtg ctgtcgctcg gcgcggtgtt ctcgatcttc gccggattct actactggat
     6841 cggcaagatg tcgggtcacc agtatcccga gttctggggg aaggtgcact tctggctgac
     6901 gttcatcggc gtgaacctga ccttcttccc gcagcacttc ctggggctgt ccggcatgcc
     6961 gcggcggtat ccggattatc cggcggcgtt cgcggggtgg aacttcgtgt cgtccatggg
     7021 ggcgtatgtc agtggcgtgg cattcctggt gttcctgtac gtcgtgttcc ggaccttcac
     7081 ctcgaaggag cacgtggcgg ataactactg gggtgacggt gcgacaacgc tggagtggac
     7141 tgtgtcatcg ccgccgccgt tccacacgtt ccaggaactc ccgcgcttcc gctgatggct
     7201 gggtcagggc gcggccatga cgataaaacg cgggcttcgc ccgaggtttc gctgatggcc
     7261 ggggccgggc ccggcccatg accaccgggt caagcccggt ggcaggccta gaggatcggg
     7321 cttcgcccga agcattttga tatgagtgag gcggttacta ccactaccac cgaagcggcc
     7381 gaactcagcg actggtttgc gttgctgaag cctcgggtga tgacattggt ggtgttcacc
     7441 gggctcatcg ggttgctgat agcgccgggc catctgaatc ccgtcctcgc atttaccgcg
     7501 gtgctgtgca tcgcagtggc ggccggcgcg tgcggcgcga tcaacatgtg gtacgatcgc
     7561 gatatcgacg cggtgatgcg gcggacccgg aaccggccca taccggctgg gcggatcgag
     7621 cccggcgcgg cactgggctt cggcgtgacg ctggccgtcg gcgcggtgat catgatgggg
     7681 ctggcagtga acatcgccgc cgcctcggtc ctggcgcttt ccatcgcgtt ctacgttttc
     7741 gtctatacga tgtggcttaa gcgccgcacc ccgcagaaca tcgtgatcgg gggcgccgcg
     7801 ggcgcgtttc ctccggtgat cggctgggcg gcggtgacgg ggtcggtgga cctggtgccg
     7861 ctggtgctgt tcgccatcgt gttcttctgg acgccaccgc atttctggtc attggcgctg
     7921 ttcgccaaca gcgattacca gcgggcgggt gtgccgatgc tgcctgtcgt ggcgggcgcg
     7981 aaggtgacgc ggcggcacat cgttgcctac acgctgctgc tggtcccgct ttcgctgacg
     8041 ccgtggctga tcgggttcgc cgggccggtg tatggcattt cggcgctgat gctgggtctt
     8101 gggtttatcg ccagcgtttt ccgcgtcgcc tccgacaagc aagacgcggc gggcgtcagc
     8161 ctcaccaatg atgcaccggc gcgaatggcg ttcaagttct ccatcatcta cctgttcgtg
     8221 ctgttcgcgg cgctggcggt tgaccggctg gtagggtgac cttggctgtg gcactcgatc
     8281 cggatgacat gcgtcggcgc cgcgcgcgca actgggcggt actgatcgtg ctgctggcac
     8341 tatcggcgct gttctatgcc atcaccatcg tcaagatgac ccatcacgga tgatgagcga
     8401 cgcgaccatg acgattcacg tgtcattgcc agcgcagcga aacaatctcg gacgagccgt
     8461 gcgcgcacaa cagcctcgag catgctgcgc gacgcttgcc acgaccgaag ggggcgcggc
     8521 gcatgcggac gtctaaccgc gccattggca tctccctggc caccgtcatc gtcggcatgg
     8581 cgggtgtgtc gttcgcagcc atcccgctct accgcgtgtt ctgctcggtc accggctatg
     8641 acggcacgcc gcagatcggc gccgccgcgg caccgggggc gaccagccag cagatcaccg
     8701 tgcgtttcaa tgccaacacg cacccgtcgc tgccctggga cttccacccg cagcaaccag
     8761 agcttcgtgt ggcgctcggc gacgagcatc tcgctttcta caccgcgcgc aacaccgcca
     8821 gcgcgcccct caccggcgtg gcgctgtaca atgtgacgcc cgacacggtg ggcaagtatt
     8881 tccacaagac cgcctgcttc tgcttcaacc agcagacgct cgccgcgggc cagcagatgc
     8941 agttcccgct gagcttctgg gtcgatccgg cgatcgccaa ggatcccaac acggcggaca
     9001 tccacacgat cacattgtcc tacacgttct tccgaacgct ggaggatgcg cagaaatccg
     9061 gtgcgttggc agaggcgggg ccgcatgtag gccctctgca gacgcaaacc ccttgaacgc
     9121 acggatgaaa ccgcgatgat gcggcacaac ggaacgagaa tgggttcggc atgagcgagc
     9181 acatacacgg cgtcaccacg atcgagagct cagggctgaa gcaaccctat catctcgtcg
     9241 atcccagccc atggccgatc gttggcggcc tggcggggtt ctgcgtggtg ttcggcatca
     9301 tcctggccgc gcatttcggc agctacgcgc tgctggtaat cggcgcattg ctcgtcttcg
     9361 tcaccatgtt cttctggtgg cgcgacgtga tccgcgagag ccgcacgccg ggcctgcaca
     9421 caccggtggt tcggctcggg ctgcgctacg gcatgctgtt cttcatcacc tcggaggtga
     9481 tgttcttcgt cggcttcttc tgggccttct tcaatttcgc gctgttgccg gagacgcagg
     9541 gcaatgggca gacggtgtgg ccgccgacca ccatccacac gttcgatccg ttccacctgc
     9601 cgttcctcaa cacgatgatc ctgctgctct cgggcaccac gatcacctgg gcgcaccatg
     9661 cgctgctgaa caatgaccgc aaggggctga cggccggact ggggctgacc atcctgctgg
     9721 gcctcagctt cacgatgtgc caggcgatcg agtactccga cgcgccgttc aagttcgtgg
     9781 gcggcggcgt ttatccgtcc accttcttcc tggccaccgg tttccatggc ttccacgtga
     9841 tggtcgggac ctgcttcctg attgtctgct ggttccgggc gcgcgccggg cagttcacgc
     9901 cggagcggca tttcggcttt gaggcggccg cctggtattg gcacttcgtc gatgtggtct
     9961 ggctgttcct gttcacctgc atctactggt ggggcgccag cccggtcacc gcgccacatt
    10021 gaccgaaccg ctgccctcgc cgcttcgtgt ggcgctgggc tgccggtgcc cgcgctgcgg
    10081 gaagggcaag ctgtacaagg ggctgctgga gatccgtgac cgctgcgatg tgtgcgggct
    10141 ggatttgcgc aagaacgaca ctggcgatgg cgccgcggct ggggtgacgc tggtgctcgg
    10201 cgcgattgtc gtggcgctgg cgttctgggt ggagttccgc ttcgagccgc cgttctgggt
    10261 gcacatcatg ttgtggccgc tgatcacggt gctgggggcg atctggttga cccgcgtggc
    10321 caaggcagca ctggtggcga tgcagttccg ccatcggtca tccgagatgg ggttgtgacg
    10381 ggcaagctgc gtcgcctgct ttggcccggc gtgatgactg tattcatgct ggccgttctg
    10441 ctggggctgg gcacatggca ggtgcagcgg ctgcactgga agctgggcat tctggcgcag
    10501 atcgcacgag ccgaggccgg gccgcctatc cccttgccgg cggtgccgac gcaattcgcc
    10561 aaggtggctg tcgccgggca tctgcgggac gatctggccg cgacgttcgg cgccgatgtg
    10621 cgcgacaccc cgggagggcc ggtgctcggc acgcaactga tcgtgccgct ggaacgtccg
    10681 ggggccgaac cgatactggt ggatcgcggc tggatccccg gcaagccgcc gcacccgatc
    10741 ccaccagtcg gcggcgacgt tacgctgcaa ggttacgtgc accccggtta cacgcccggc
    10801 tggttcagcg cggccgacaa tccgggggcg cggcagttct atacgctgga tcctgccgcg
    10861 atcggcgcgg cgctagggct gccgcgtgtg gcgccgttcg tcctgatcga gctgggttcg
    10921 gcgccacctg gcggctatcc gctgccggcg cagcacttgc cgcagccgcc gaacaaccac
    10981 ttgtcctacg cgatcacctg gtatggcctg gcggtcgcgc tcgtggtgat ctttgtgatc
    11041 tgggctagaa aggtattggc tgaatgactg cttacgaccg gctggccgcg cggtttgcgc
    11101 gcatcgcgac actcggtgag gcctcgtcgg tgctgaactg ggatgccgcg accatgatgc
    11161 cgcccggcgg aggcgccgcc cgtggcgatc agctcgccgt gctggcaggc atgtcgcatg
    11221 ccctgctggt tgcagccgag gttggcgacg atctcgccga agccgaacag gccggcgaga
    11281 acgatccgtg gcgcaccgcc aatctccgtc tgatgcgtca tgcctatacg cgcgcgacag
    11341 ccatgccggc ggacctggtc gaggcgcagg cgcgcgccaa ctcggcctgt gagaaggtct
    11401 ggcgcgaggc gcgccgtcag tcgaatttcg ccatggtgcg gccgcacctt gaggaagtgg
    11461 tgcggctggt acgtgaggcc gccgccgcgc tggcgccggc actcggcctc agtgcctatg
    11521 atgcactggc cgatggatat cagcggggaa tgcgtgcggc cgaagtcgct ccggtgttcg
    11581 ccagctatga ggctttcctc gccacagccc tgccacgcgc cgaggaattg caggccggcc
    11641 gtcccgtgcc cgttcgtcct cacgggccgt tcccgatcac gaggcaggaa gccttgtgcc
    11701 gggagctgtc cgagcggctc gggctggatt tcgcccacgc ccgcctggac cgttcggcac
    11761 accctttctc cggcggcacg gtaacggacg tgcgcatcac cacgcgatat gatgaggccg
    11821 acttcacgca ggcggtgctc gccgtggtgc acgagaccgg ccacgcactg tatgagcgcg
    11881 gcctccctgg cgattatgcc cgccaacccg taggtgaggc cgccgggatg gcggcgcatg
    11941 agagccagtc gctgatcgtt gaaatgcagg cctgccgctc ggacgccttc ctctcgtatt
    12001 tcggccccaa gctgcacgcc gcctttggca acgacacaac gccctaccag ccggagaacc
    12061 tggcgcggct gtggcggcgc gttgagcgta gctacattcg tgttgatgcc gacgagatga
    12121 cctatcccgc acatgtgatc ctgcgcttcc ggctggagca ggcgctgatc gatggggcgc
    12181 tcgctgtggc cgatctgccg ggcgcatgga atgaggcgtt ccagcgactg ctcgggatga
    12241 cgccgccgga tgatgcgcgc ggctgcttgc aggacatcca ctggtatgac ggcgcgctcg
    12301 gttatttccc cagctatacc ctgggtgcga tggcggcagc gcaactgatg gcagcggcga
    12361 gacgcgccgt tccgtcactg gatgcctcgc tggcacgggg agatctgtcg ccgctgctgc
    12421 attggctgcg cgccaacgtg cacggcaagg gcagtctgct ggagttcaat ccgctgctgc
    12481 aggcggcaac cggcgagccg ctcaatccgg cgtatttcca gcagcatctg acggcgcgct
    12541 atctgcaata gcggtcttgc caaactttgc ggcgccacgc atgacggtgc ccatgcaaat
    12601 ccaacgtgcg gccgtcgtcg cggccttctg cgccgtgctg agtgcctgcg cgtcaaagcc
    12661 gccccctgcg ccggagccca aaccgccgcc accgccggaa atcgacggcg aataccgcgg
    12721 gaccaccacg cgctttcagg ccgacaccaa ggcctgtccg catcctggat tggtcgactt
    12781 ccaggtgatc cagcatcagt tccagtatcg ctgggactat gacaccttcg ttctggcaac
    12841 catcgcaccc gatggcggcg tcaccggcgg cgccgagcgc atcacactgg tgggcaagca
    12901 gaacgggccg atcatcgagg gcgatgtcac caacggcaat tgcggtctgc atttcacggt
    12961 gaagctgcac ccggcgccgt aattgctggg gagcgggcgc cgcagtaccc tgcgcctcat
    13021 gcaatacgtc tccacccgtg gcaccgctcc tgtgcgcgac tttgccggcg tattgctggc
    13081 cggccttgcc gaggatggcg gcctttacgt cccggaaacc tggcctgtct tcacaccagc
    13141 cgactggcgc gcccttcggg ggcttcccta ccacgcgctg gcggcacggg tgatccagcc
    13201 gttcgtcggc aaagcgatcc ccgccgcaac cctcgccgcg ctgtgccgcg aggcatatgc
    13261 cggcttcggc catccggccg tggtgccgct cgtccagctc gagaccggcc tgttcgtaca
    13321 ggagctgttc cacgggccga cgctgtcctt caaggacatg gccctgcagc tgatcggccg
    13381 cctgttcgac catgtgctga cagaacgcga cactcgcgtg acgatcgtcg gggccacgtc
    13441 cggcgatacc ggatccgccg cgatcgaggc ctgcgccggg cgcgaccgcg tcgatatcgc
    13501 tttcctgcat ccgcaccatc gcaccagcga agtgcagcgg cggcagatga ccagcgtcac
    13561 tgcgccgaat gtcgccaaca tcgcgatcga tggcacgttt gacgattgcc aggacctggt
    13621 gaaggcgatg ttcgccgata cgccattccg gcaggagatg catctgtcgg cgatgaactc
    13681 gatcaactgg gcgcggatcg cagcgcaaat cccctactat cttgcgtcgg cgctggcgct
    13741 gggcgcgccg gaccgggagg tggcgttcag cgtgccgact ggtaatttcg gcaatatcct
    13801 cgcggcgtgg gtggcgcgac ggatgggatt gccgattgcc aggctgatcg ttggctccaa
    13861 tcgcaatgac atcctggcgc gcttcctgac aagcaacgac atgtcgatca cctcagtgga
    13921 accgagcctg tcgcccagca tggacatcca ggtcagttcg aatttcgagc gcctattgtt
    13981 cgaactgctg gagcgcgatg cgcaggcgac cgccgcggct atggaagcgt tccggcgcac
    14041 cggccgcatg acggtgccag atgcgcaatg gcatcgctgc cgcacggtct ttcacggctt
    14101 ccggcttgat gacgcgggaa cggaagcgga aatcgctcgg gtgtaccagg caacgggcta
    14161 tctcgccgac ccgcataccg cgatcggcat cgccgcggcg cgcgccaatc ctgtgcatgg
    14221 agtgccgatt gtcacgatgg cgaccgcgca tcccgcgaag tttcccgacg tgatcgcgcg
    14281 cgcaaccggc attcatccgc cgttgccgcc gcgcctgacc gacctgtacg agcggccaga
    14341 gcagttcagt gtgctgccca acgaccttgc cgcggtggag gccaaggtgc gcgcgctggc
    14401 gcaccgtaac gcatagaaag gaaatcgcca tgagccaaag tctcgaaggc gtacgcgtgc
    14461 tggagctggc gcgttatcag gctggtccgc ggggcggcat gatcctctcc gatctcggcg
    14521 ccgaggtgat caagatcgaa cggatgggcg gcgaggagac gcggcatcac gcgcccatgg
    14581 tgcgcgggca gagtatatac ttcacggtat acaatcgtgg caagaagagc atctgcctcg
    14641 atatgcggca ggagcgcggc aaggagatct tcaccggcct ggtgaagacc gcggatgtcg
    14701 tgctggagaa tttccggccc ggcacgatga aggcgatggg tttcgattac gaaaggctgc
    14761 gcgcgatcaa tccgcgcatc atcctggttt ccgtgtcggg cttcggtcag tacggcccgt
    14821 acaccgatcg gccggcgttc gatccgttgg gccaggcgat gagcgggctg atgtcgctga
    14881 ccggcaagcc ggtggggcag ccactgggca cggcgtcgtc cgtcgtggac cggtataccg
    14941 cgctgcatgc caccatcggc acgctggcgg cgctgcgcca tcgggatcgt acgggtgaag
    15001 gacagctggt ggattgctgc ctactggatt cggccctcac aatggtggag atacccacgt
    15061 cctattacct gtccaccggc gaggagggcg gtgaaggcgg acggccgccg tatcgcgcca
    15121 aggatggcta cgtggtgatc tcggcgggtg ggcgcgagat ggcggcgcga ctggcacaac
    15181 tggtatccag tggaaccata gccatgagcg gcgagccgat cacggcgggt cagggcggca
    15241 gcgatacccg tcgcgcgctt gaggcttggt gcgctgaacg tccggtggaa gagatctgct
    15301 ccgcactgat ggagatcggc gttcccgtgg ccccggtacg cacaattccc gaggtggcga
    15361 aagacccgca tctgtgggaa cgcgagatgc tggtgaagat ggaggatgcc gtcgccggtg
    15421 aactctatgt accgggagtg acagtgaaga tgtcgaagac gcccggacgc gtggggcccg
    15481 tcccgacgcc gggtcagcac acggatgaac tgctggggca gctgctgggc tacgacgccg
    15541 atacgctcgg cgcattgcgg cgtgacgcta tcatcgcttg agagcagtct ggtccacaga
    15601 ttgcattgat tatccccagc aaaccggagg cgatgagatg gcactgaccg tggcgaccgg
    15661 ccaggagatc gacggcaagg cgttctggac gacgctaggg cagcgcgcga ccggcatgac
    15721 ggtggtcacc gccgatggcg atgacgggcc aaccggcttt ctcggcctct cggctgcgca
    15781 tgtgacggcc agtcccccca ccatgctggt ctcggtcgat cgcaagacca gcgcactggc
    15841 aggcatcctg gcgcgccggc acttcgcggt gaatttcctg cccgcagatg ccggccaggt
    15901 ggcggatgcg tttggcggcc gcgctggggt ttccggcgcc gatcggttcg cgcccggagc
    15961 atggcgcacg ctggcaaccg gtgcgccagt ctacaatgac gctctcggtg ccttcgactg
    16021 cacggtggcg caggtcgtgc agcacggcga tatctcgatc atcatcggca cggtcgtcgc
    16081 cgcggcatgg cgcccagatg gggagcccct ggtgtttttc cgtgggaaga gcttcaccgg
    16141 aatgggcggc gcagcatagc gcagtggtcc ccctgtcatt gcgacgagcg gagcgacgtg
    16201 tcagtccgcg aaccccatcg catcattata agcgcgacgc accgcatccg gctgcccgac
    16261 caggaatgga aactccctgc gcacaatctc gtcgcgctcc agaccctcga caaaactctt
    16321 ccgcccgatg atcggcagtt cgatgcagcg gttcacctcc gcatcgcgca ccacggcctt
    16381 tggatcgtcg ccgctgccca gtgcctccag atcgcggaag aaacgctggc gcatcatgat
    16441 gatgccacgg tcgctgcggc cgagatgctc gcgcttgcga tcggtgatcg cgccctggcc
    16501 gacccagcct atgaaatcct ggttcatgat gtggctggtg atccaccggc cggacttctc
    16561 gtcggtgatc ggtgaatacc agtatggaat gctgttctgc acgtaaggtt cgcggtctct
    16621 cggcactcgc gtgaaaaacc agccgacact cagcgtgttc gtatcatcga tcggcacccg
    16681 ccactcgaag tgattgccgg tgaacagcgc gttcggccag aggcagacgc ggccaaccgt
    16741 ccaaagcgga ttcttctcgt ccgagtcctc gcgcacccgc cgataggtga tgccgtattc
    16801 gaactcatcg aacgccagct tcaggtggcg cggcgcatag tcattgcgcc ccttcagccg
    16861 tgcgccccag ttatcgtgca tccattcgaa atgcaccgga tcgatcgagt tctcctggca
    16921 ctggaaccag ttgcagggca ccgtcgcgaa cacgatctgc acgaagccgt tgtcccaggt
    16981 gaacggctcc cagttcggca ccagcggtgc cggttccggc ccgagatacg cccagagcaa
    17041 tcctgccttt gcctccaccc gataggcctt gatacggatc tgttcgcgga agcggatttg
    17101 cggcgcgtcg atgtcctcga acggctgctc caggcatgcg ccgtccgaag caaacatcca
    17161 accgtgataa ttgcaacgta gaccgcaatc ttcaacgatg ccgtagctca gatcggcgct
    17221 acgatgcggg caatgacgct ccagcaatcc gtagtggcct gaaagatcct tgtacagcac
    17281 caggtcttcg cccagcagcc gcaccgcctt ggtcggcgca tcatccagtt ccgagaccgc
    17341 tgctatcggc tgccaatagc ggcgcagcag ggcacccatc ttcgtgccag gcccgacttc
    17401 gcagagcagc cggttgcgtt catcactcaa catcagcgac ccctgacgtc agttcgattg
    17461 cgtgattgcg ggaaaccata cctcggcccg gtgcgccggc aggcccttgt agcggccggc
    17521 caatggaacc accgcgaaat tcgcgtcggt gccattcact cgcggcttgc tgccggtgac
    17581 tttcgcatag gcttcagtga gcatgcaggc gagccattcg gcggcactct tctgctctgc
    17641 gcgatagtag cggacctgtg gcgtttgcct ggccggcgcg gtggccgcca caagctctat
    17701 gccggcagac ggaaactccg ccggcatctc gaacccgatg cgggcggccg aatcccggtc
    17761 gttggcctgg acgatctgat agtagacgac aagcggcagt gtcgcggtgt cgccgggccg
    17821 agcgcagaga ggtgccgcgg ccgctgtggg cggcggcgcc gttggcactg ctttcggtgc
    17881 tgcggccgcc ttcacttgtg gcgcaggcgc cggagcggtc ggcaccggcg acggcactgc
    17941 ggtcggcacc ggcgctggag cgggcggcgg cggcgctgcg actggggcta cggctggcaa
    18001 atactctggc gactgactct cctgcttcgc ctgtgccacg gcgatggaaa tttcattctg
    18061 ggtatcgaag atagcgtcat cggtacattc tgtgagggcc aatagctcgg gatgctgctt
    18121 gacgatcagc gcgcggatat gctgcgcggg tgcgatgaag tccgcatggc cgggcgatgc
    18181 gatgatgacc gcgttgatga ccgtgcaagc ctgttgtggc ggcaagagcg tgcttgcata
    18241 ggtcagtgcc tgggtgcgtt tctcgatatc ggcggacagc agcaactcgg tctgcgccgt
    18301 ggtgaattcg acgagcttct cgccgctttc gatgcgcttc agctccagat cgcgcagcgc
    18361 aagcagatcc ttcgatttcg agtcctgcac agcgatatag gtctgcgccg cggcaacaac
    18421 gaatgcggcg attggcaccg ccaccgcggc agcgcgcgcc cagaatgaca gttcccagcg
    18481 caacttgcgc aattccagcg cgaccttgtc cgaactgtct ggttcgtccg acatcgctac
    18541 ctccaaccat caccagccag ggcgtcagga gagcttcacc aacttctgca tgcagcggat
    18601 gtcctcttcc ggcgcgatcg gcgtcccgcg gttgcgcatc gccgtgcgcg acacttcgcc
    18661 cacgtagata ttgccgcgtg agtcgatggc aaccccatgc ggcgcgatga actggctgga
    18721 gcgttcgcgc gtcgcatcgc ccagccgtgc gatcggtttg ccctcatgtg ttacgattga
    18781 aacgcgcggg ccaatccccg gcgacttcgc gttgaccgcc atgccgggac cgctctcccc
    18841 gatatacgcg atcggattcc tgccctgcgt catgcacagc ccgcaaggtc gatgcaggtt
    18901 gttccactgc gactggtatt ttcccttgcc gtcaaacacc tgcacgcggt ggttctcgcg
    18961 gtccgcgacg tagacccagc catccgcatc acagcagata ttgtgcacga tgttgaacgc
    19021 gccggggtca gtgcccggct cgccccagga aaattgcaac ttgccgtccg gtgaatactt
    19081 gtggacgcgg gcgttgccgt agccgtcagc gacatatagg tcgccctcag gcgaaagagc
    19141 ggtgtgagta caccggttga acggatcgcc gccatacatc ggtgccggtt cgcctggaac
    19201 gccgatctcc agcagcagct ttccctcgag cgtcaggcga cgcaccgtat ggtcgccgtc
    19261 atcggtgcaa tagatcgtat cgtcgggtgc caggtgcacg ccgtgcggac gcttgaacag
    19321 gccctcgccc caggaacgca ggaaattgcc ctcgccgtcg aacaccatca tcggatgctc
    19381 gctgcgtgcg aagacgtaga cattgtcctt gctgtccacg ccgactgccg cgacttcgcc
    19441 atagcgccag ccatccggca gcttgcccca gttctcgatc acctcgtaac ggtgctcgcc
    19501 tgagccgacg atgatcgcca tgttccctct cccgcggttt tgggggagcg tagcaccatt
    19561 gcgcgcgacg gccagtgtgg gcagcagagg aggttcggca tgactgatac ccattacgcg
    19621 agctatacct tcaacgactt tcgtttgcag aacggcgagg tgatgccgga gatcaccctc
    19681 gcctactgca cgcgcggcac gttggcgccc gatgggcgca acgcagtgct ggtgacgcac
    19741 ggctatacca gcagccatcg catgatcagc tccggaactg gttcgtccga gggcgcctgg
    19801 agcacgctgg tggggcccgg tgcgccgatc gatacggggc gcgacttcgt cgtgtgttcc
    19861 aacatgctgg ggtccagtta cggctccacc aatgccgcgt cgcgcgatcc gcgtaccggt
    19921 aggccgtacg ggtcccgctt cccgaatatc acgctgccgg acatcgtcac cgcgcagcgg
    19981 catttgctgg atcatctcgg ggtgaagcac ctgcgcgcgg tggtcgggcc atcgtatggc
    20041 gggtttcagg cgtttcagtg ggctgtgacg tttcccgatt tcatggacgg aatcgttccc
    20101 gtggtgacct cgcctcgtgc gcaaagtacg gatcgcgtgg cgacattgcg ggcgcgcttc
    20161 gagcgcaatc cgaactggca tggtggcgac tattacgaca cgccaggcat gttcgactcg
    20221 ctgcttgaac tgcgcattga gacgctgaag cgctacggca tgcctggttc agacgaggag
    20281 atcacccggg tagcgtcggt ctgggcgcgc gaattcgacg ccaattcgct ggtgatcctg
    20341 gggcgcgcca tggaagccta cgacgtgacg cctgagatgc gccgcatccg cgtcccaatg
    20401 ctgtatgttc tatcccgcac cgatgcgctg ttcccgccgg gccttgcgcc cggggtgatg
    20461 gcgagcctgc gcgacgcggg tgtcgaggcc agctatttcg agatcgacag cgagctcggg
    20521 cacacggcgt cggggaccga tgcggcaaag tgggcatcgg ccctgcgcgg cttcatggcg
    20581 cgccttccat agcttatgct tcgggtgcca cgcctacata cctgtattga tgcaccagat
    20641 cgtccaccgg atgcgccgcg tcgtgcttca cctggaacac cgcgacgacg cgctgcctga
    20701 tatcgccgtt gtcgtcaaag ctgacctcgc cttgcagcgt cttcagcttt gtcgatgcga
    20761 tggcgtcccg caccgcgaca cgttccacag gcttgccgga tttcgccaca cgctcgaccg
    20821 cgttgatgac gaccatcgcc gcgtcatagg cggtgatgga gtaatccgac ggctgtcggc
    20881 cggtcttggc ggtgaagcgc tttacccagg gctgtagctc cgtatcctcc atcatgtgtg
    20941 ggcttgcgac cgttgcgtac cagccatccg ccgacggaaa tccggcgccc tggaggatag
    21001 acggaccata catgccatca ccgccggcct tgatcgcatt agggatgaga tcataggcct
    21061 gcttggccag cttcacgccg gcctgggcat cgccgccgta gtagatcgcc tgtgcgccga
    21121 gcgccttgat cttcgtcagc gccggcgtgt agtcggcagc cttcggatcc agacggtcgt
    21181 ggcccagtac ctggatgccg cgcttcttcg cctgtgtctc gaacgcggtg gaaatgccga
    21241 taccgtaggc gccactgtca tcgagaccgt acacactctt caccttcagc gtgtcggcga
    21301 agtagttggc catgttggga ccctggtagg cgtcggtggt gactgtgcgg aagtagacgg
    21361 ctttgcccgc cggccgataa agcgcggcaa acttcggatc ggtgatgtca gggttcgtcg
    21421 acgtcggcgt gatcgtcgcg agattgccct ggctgaatat tggcgacatt gccttgcctg
    21481 gcgtgctgct catcggacca agcgcggcga cgaccgaagg atcggccacc atcttgcgtg
    21541 cattggtggc gccctgtgca ggatcgtact gtcccgctgt cgctgtgccg tcatccagca
    21601 ataacacgtt cagatggtag ccggcgggtc cgcctttctc attggcctcg tcgatcgcca
    21661 tcatgaagcc gtcgcggata cgaacggcgg tctcggcatc ggcgccggtg agcgacagat
    21721 cgatgccgat cgtgactgat ttgtcggcgg cgtatccgcg ccgcgtgcca agcgtcaaca
    21781 gcgcagccga tgccccgatg tggaaagcgg cacggcgagt gatgtgcgtc atgaccttac
    21841 gcccccattt gttgattatg ccccagccta gccgtgggat catgcgcact caagtttgct
    21901 gtctgacaca ctgtcattgc gaggtctccg gcgaaggccg gagaacgaag caatctcggg
    21961 ccatacagct cggtattctc ttgccacgtc gcttcgccgc gtcacgcgac accatcgact
    22021 agctgtcatt cgtgagcgtt ggccacaacg cgtcggcgcc gccactacaa acataggcac
    22081 caacctttgc tgcccattcg gtgtccccac cgaactcgtc gcgccacgac cacaagcgcc
    22141 gcgtggacag ttgcagcgag tattccttgg tgaaaccaat cgccccatgc acttgatgcg
    22201 caatctctga cgcgatcgtc gccgcatcgc ccacgcgcgt tttggcaatc ccgaccagga
    22261 actcctgctt cgccggatcg ctcacggcaa gcgcctccac gccggcatca gcggccgcac
    22321 cggccgccgc cacttgcccg gccagcaccg caaggttctg ttgcacggcc tggaacttgc
    22381 cgatcgggcg gccgaactgc ttgcgctcgc cggcataacc gacggcggta tccagcgcgc
    22441 gttccattgc accggccatc atcatggcgc gagcaagtgc gccgcgccgg tagagcgccg
    22501 cctggtcgat gcccgctggc gctggcgcaa cctcgttgcc ggacaacccg gcgccagcaa
    22561 attcatacgt atcgcgtggc tcaccggcca tgttggcacc gcccgacggc ggtgttgctc
    22621 cgggaatgcc atcaccgaac accaccatgg tgttgccctc agggtcagtc gcaataagcg
    22681 cgacctggcc agcctgttgg ccccagggca gccggctcgc gcgcccgaac agccgccagc
    22741 cattgccgtc gcgttgcagc gtcagcttgt cggcggcgcg cacgggaccg acggtcatcg
    22801 gcccctcgcc cggggtgaaa ccggcggacg atgccagcca cgcggctagc atcgtttcgg
    22861 cgagcggtat cggcgcggca tgacgccccg ccacgcgcag cgcggcgtag agatcggacc
    22921 attcgacctc cgagccggca acgcctgcga gcggtactcc ggtatcgctg acggcctgcc
    22981 acagtgcggc agggaacgtg cccgcttcgg cggcatcggc gacggccttg gtgcagtggt
    23041 cctcgaacag gcggcccatg ctttccagca gcatgcgtgt ttcatcgcgc atcggctcgt
    23101 cctccttcag cgcaggccaa ggccgcgcgc gatgatgccg cgcaggatct cgcgtgtgcc
    23161 gccctggatg gacagtcgtg gcgcgttgcc gatgccgcgc gccagcatag acacgtatgt
    23221 atctaccgcc tgcagatctg gttcgtcggg gaacagttgc cgggcaacaa ccggcagatc
    23281 ctgttcgtag cgcgtgccca ggtccttggt caccgcggcc tcgttcagcg gttgcagccc
    23341 gtcctgcagc atgccggcga tcgacagcga catgcggcgc agcgtggcga gctgcgcaac
    23401 cagccgcccc aatgctgcag ctgtcgcgcg atcgggattg ggcccggcca cgcgaaccat
    23461 ctccgtcagc aacttgaaca gcacgaggaa acggtccggc ccgctgcgct cgaacgcgag
    23521 ctcgctggat acctggcgcc agccattgcc gatttcgccg aacagccgat tgtccggcac
    23581 gaacaggtcg gtgaggaaga tctgattgaa gtgatgctca ccgagcagat tgatcaccgg
    23641 cctgatctcg atgccgggcg agttcttcag gtccaccagg aactgcgtcg caccggcgtg
    23701 gcgatcgacc tcctcctgcc cgtcgctcgc catcttgcag aacaggatca tgtagtcggc
    23761 gcgatgcgcg ttcgacgtcc acagcttgct gccgttgatg cggaagccgc catcgacctt
    23821 caccgcgcgt gtcttggtgc ccgcaaggtc agagccggaa
//
LOCUS       DATGEM010000307         5433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_920274, whole genome shotgun sequence.
ACCESSION   DATGEM010000307 DATGEM010000000
VERSION     DATGEM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920274"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VKQ27_09835"
     CDS             complement(<1..1072)
                     /locus_tag="VKQ27_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY89275.1"
                     /translation="MPTSATDFSIPFLRGRRTNKWHKFADDVLPAWVADMDFGVAPPI
                     AEAMARLARNQEYGYAAREGLLAESFARRMQARFNWTVDPADAHPIGDLVQASFSSVM
                     AFSEPGDAVVLQLPSYPPFMRAIEDTGRRLIANHMRDNGTRWELDLAAYDAAADPRAR
                     VLIFCHPQNPTGRAYTRGELEQVAAFVLRHDLIVVSDEIHADIVYPGGTHIPLASLSP
                     EMAARTITITSATKSFNIPALRCAVMHFGTPALKERFFARIPSRLLGSPGVTGVDATI
                     AAWDEGQPWLDEILGQLQANRDWLMQNLGEALPGVTLRRPEATYLAWLDCSALKLPVP
                     AGQFFLDSARVGLNAGETFGERY"
     gene            1202..1753
                     /locus_tag="VKQ27_09840"
     CDS             1202..1753
                     /locus_tag="VKQ27_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HLY89276.1"
                     /translation="MAQGALDELAGLLEGKGARRYGLSAVNQLQHALQAALLAEQSGC
                     DAALITAALLHDIGHMVHGLGENPAEDGIDDRHEELGRAYLAPLFGPDVTEPVRLHVP
                     AKRYLCATEADYFGQLSPDSVLSLKLQGGPMSDAEVAAFRALPHSTAAVQLRRFDEGA
                     KVAELATPPVAHFLPYVQACLRA"
     gene            1844..2809
                     /locus_tag="VKQ27_09845"
     CDS             1844..2809
                     /locus_tag="VKQ27_09845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLY89277.1"
                     /translation="MPFIRTALTELLSIEHPIMLAPMGSAAGGRLAAAVSHAGGLGLI
                     GSGYADSETIKRELAAAGNARVGVGFIQWALDDKPEALDVALAASPPAVMLSFGDPSR
                     HAGAIKAAGSRLICQVQSLAGARQAAEAGADIIIAQGRDSGGHSGTVRGTMGLVPAVV
                     DAVAPIPVVAAGGIANGRGLAAALALGASGVLMGTRFTASQESLWQDVLKQGAVDASG
                     DNTEQTRVFDIVRGAKWPAHFPGRVVKNAFFAKWHGREDELANAWQDEEKHYLDAPAG
                     DLATRVLWTGEGVDLVNDIPPAAAIIERIVAEAIAVLRRGAAQIR"
     gene            2878..3474
                     /locus_tag="VKQ27_09850"
     CDS             2878..3474
                     /locus_tag="VKQ27_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89278.1"
                     /translation="MNEDRARQTDIAMAEYRMLRDEIASATNRRFEIWKYGIPVVGLL
                     LAYGAKSISEPADGWSGYYIIGALTPALIWLLISLWTGEYLRSQRAGYYCRRLEQKIN
                     TWHPDSLGWETFLSPTKGKAKHETYDAELRFAQCICYVLYVIGLSFLFKRPSFLLIDW
                     LIAILAVFVIAFAHVELSRRVRRTMADAWRYNSALEPE"
     gene            3593..4153
                     /locus_tag="VKQ27_09855"
     CDS             3593..4153
                     /locus_tag="VKQ27_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89279.1"
                     /translation="MQDTLTWRAPTIAPQQLDTLIAFSFGNRLQPNGNSEPGPTNEAL
                     AKALVSVHRIKPVHVYAQWEVAEAVGDLIPEATISSIYPRRGNNAEMIYLSTDGVLAA
                     IVTDAGPAHVLGKVGIIAFSDHQFRCVTTARRRGLDAYAPADIAMPSTYDPESGQPWC
                     RTRMAYLLHDIGERISARRAEAIGMG"
     gene            complement(4288..>5433)
                     /gene="groL"
                     /locus_tag="VKQ27_09860"
     CDS             complement(4288..>5433)
                     /gene="groL"
                     /locus_tag="VKQ27_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423234.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HLY89280.1"
                     /translation="MQKVGNEGVITVEEAKGIQTELDVVEGMQFDRGYVSPYFITNAE
                     KMIAELDQPYVLIHEKKLSGLQPMLPLLESIVQSGRPLLIIAEDVEGEALATLVVNKL
                     RGGLKVAAVKAPGFGDRRKAMLEDIAILTGGTVISEDLGIKLENVKLNDLGKAKKVLI
                     EKENSTIVEGAGKKQDIVGRCNQIRAQIEETTSDYDREKLQERLAKLAGGVAVIRVGG
                     STEVEVKERKDRVDDSLHATRAAVEEGIVPGGGVALARASLILAKLKADNDDQRFGVE
                     IVRKAIMMPLRQIAENAGEDGAVIAGKVLDKDEYNWGFDAQTGEFKDLVKSGIIDPTK
                     VVRTALQDAASVAGLLVTTEAMVAEKPEKKAPMMPPGGGGGMGDMDF"
BASE COUNT          945 a         1789 c         1715 g          984 t
ORIGIN      
        1 cataccgttc gccgaacgtt tcgcccgcgt taagccccac gcgcgcgctg tccaggaaga
       61 actgtcccgc cggcaccggc agcttcagcg ccgaacagtc cagccacgca agataggtcg
      121 cctccggccg ccgcagcgtg acgccgggca gcgcctcgcc caggttctgc atcagccagt
      181 cgcgattggc ctgcagctgc cccaggattt cgtccagcca gggctgcccc tcatcccacg
      241 ccgcgatcgt cgcatcgaca ccggtgacgc ctggcgagcc gagcagccgc gacgggatgc
      301 gcgcgaagaa ccgttccttc agcgccggcg tgccgaaatg catcacggca cagcgcagcg
      361 ccgggatgtt gaagctcttg gtcgccgacg tgatggtgat ggtgcgcgcc gccatctccg
      421 gcgacagcga tgccagcgga atatgcgtgc cgccgggata gacgatgtcg gcatggatct
      481 cgtctgacac cacgatcagg tcgtggcgca gcacgaacgc cgcgacctgc tccagctcgc
      541 cgcgtgtata cgcacggccc gtcgggttct gcggatggca gaagatcagc acccgggcgc
      601 gcggatctgc tgccgcgtcg tacgccgcaa gatccagctc ccagcgcgtg ccgttgtcac
      661 gcatgtggtt ggcgatcagc cggcgcccgg tgtcctcgat cgcccgcatg aacggcggat
      721 aggacgggag ctgcagcacc acggcatcgc ccggttcgct gaacgccatc accgaggaaa
      781 agctcgcctg cacaaggtcg ccgatcggat gggcgtcggc cggatcgacg gtccagttga
      841 agcgggcctg catgcggcgg gcaaacgatt cggccagcag cccctcgcgc gccgcatagc
      901 cgtattcctg gttgcgcgcc agtcgtgcca tcgcttcggc gatcggcggc gccacgccga
      961 aatccatatc ggcgacccag gccggcagca cgtcatcggc gaacttgtgc cacttgttgg
     1021 tgcggcggcc gcgcaggaag ggaatgctga agtcggtggc actggtgggc atgcggttcg
     1081 ttctcctgtt gcgcgccgta tgcgccttag cgcgcggcgg gcgcggcgtc gagttggccg
     1141 cggctgacag cggcacccgg cctggactag ggtgccgccg cattggtcgg gatgatggga
     1201 catggcccag ggcgcgctgg atgaactggc tggactgctc gaaggcaaag gcgcccggcg
     1261 ctacgggctg agcgcggtga accagttgca gcacgcgctg caggcggctt tgctggccga
     1321 gcagtcaggc tgcgatgcag cgctgatcac cgcggccctg ctgcacgaca tcggccacat
     1381 ggtgcacggg ctgggcgaga accctgccga ggacggcatc gacgatcgcc acgaagagct
     1441 cgggcgggcc tacctggcac cgctgttcgg ccccgacgta acggaaccgg tgcgcctgca
     1501 cgtccccgcc aagcgctacc tgtgcgcgac cgaggcggat tacttcgggc aactctcgcc
     1561 ggacagcgtg ctgagcctga agctgcaagg cggcccgatg tcggacgccg aggtggccgc
     1621 cttccgcgcc ctgccccatt cgacggccgc ggtgcagctg aggcgcttcg acgagggcgc
     1681 caaggtggcc gaactggcga cgccaccggt cgcgcatttc ctgccgtatg tgcaggcgtg
     1741 cctacgcgcg tagcggcggt cggcccaggc tccgcgtgat attggcgacc ggatagaaca
     1801 gtctgtccgg caaccaccga taattcatgc gagggaggtg ttcatgcctt tcatcaggac
     1861 cgcgctcacc gagctgctca gcatcgagca tcccatcatg ctggcgccga tgggctccgc
     1921 cgcgggcggc cgtctcgccg cagcggtcag ccatgccggc ggtctgggcc tgatcggttc
     1981 aggttatgcc gacagcgaga cgatcaaacg ggaactggca gccgccggca acgcgcgtgt
     2041 gggcgtgggg ttcatccaat gggcgctgga tgacaagccc gaggcgctgg atgtcgccct
     2101 cgcagcgtcg ccgccagcgg tgatgctgtc gttcggcgat ccgtcgcgtc atgccggcgc
     2161 gatcaaggcg gcggggtccc ggctgatctg ccaggtgcag tcgctggcgg gcgcgcgtca
     2221 ggcagcagag gcgggcgccg acatcatcat tgcgcaaggc cgcgattccg gcggccattc
     2281 gggcaccgtg cgcggcacca tgggcctcgt gccggcggtc gtcgatgcgg tggcgccaat
     2341 tccggtggtg gcggcaggcg gcatcgccaa cggccggggg ctcgccgcgg cgctggcgct
     2401 aggcgcgtcc ggcgtgctga tgggaacccg gttcaccgcg tcgcaggaat cgctctggca
     2461 ggacgtgctg aagcagggcg ctgtcgatgc gagcggcgac aacacagagc agacgcgcgt
     2521 gttcgacatc gtccgcggcg cgaagtggcc ggcgcacttc cctggccgcg tggtgaagaa
     2581 cgcgttcttc gccaaatggc acggacgcga agacgaactg gccaacgcct ggcaagacga
     2641 agagaagcac tatctcgatg cgcccgccgg cgatcttgcc actcgcgtgc tgtggacggg
     2701 cgaaggcgtg gacctggtca acgacatccc gccggccgcc gcgatcatcg agcgcatcgt
     2761 ggccgaagcg atcgctgtac tgcgacgcgg cgcggcacaa atccgctaga gccgtcgtca
     2821 gcttcaccgg ttgacctaca tcaatgcgcg atatcgcgcc tgtcggtaac gacaagcatg
     2881 aacgaagatc gcgctcgtca gacagatata gccatggccg agtaccgcat gcttcgggat
     2941 gagattgcta gcgcgaccaa tcggcgtttc gaaatctgga aatacggcat tcccgtagtc
     3001 gggctgctgc ttgcctatgg tgcgaaatcc atttcagaac ccgcggacgg ctggagcgga
     3061 tattatatca taggagcgct tactcctgcg ctgatttggc tgctgataag tttgtggacc
     3121 ggcgaatacc tgcgatctca gcgggcgggc tattactgta ggcgtctgga acaaaagata
     3181 aatacgtggc atcccgattc tctaggctgg gagacgttcc tgagccccac gaagggcaaa
     3241 gccaagcacg agacttacga tgcagaatta cgatttgctc aatgcatatg ttatgttttg
     3301 tatgtgattg ggttgtcctt tctatttaag cgcccatctt tcctcttgat cgactggctg
     3361 atagcgatct tagcagtgtt cgtcatcgcc ttcgcgcacg tcgaactgag ccgccgcgtc
     3421 cggcgcacga tggccgacgc ttggcgttac aactcggcgc tcgaaccaga atgaaacgtc
     3481 tctgagggca atggagattc cgggagtgac cgctgtatca tctgcgatcc gccaccatct
     3541 gaattccgag ctcggagacg cccagaccgc cagccaacta gcggagctac tgttgcagga
     3601 caccctgacc tggcgagccc ccactatcgc ccctcagcag ttagacacgc taatcgcctt
     3661 cagcttcgga aaccgcttgc agccgaatgg caatagcgag ccaggcccca ccaacgaggc
     3721 cttggccaag gctctggtga gcgtgcatag aataaaacct gtacatgttt atgcccaatg
     3781 ggaggttgcc gaggccgtag gagatctgat cccggaggcc acaatttctt ccatttaccc
     3841 tcggcgaggc aataatgctg agatgatata cctgagtaca gatggtgttt tggcagcgat
     3901 cgtgacggat gccggaccag cccatgtgct tggcaaggtc ggaatcatcg ccttctccga
     3961 tcaccagttt cgttgcgtaa ccactgcacg gcgccgtggt ctcgatgcgt atgctcctgc
     4021 cgatatcgcg atgccctcga cctatgatcc cgaatctggc caaccttggt gcaggacgcg
     4081 catggcctat ttgctgcacg acataggcga gcggatttca gcccgccgcg ctgaggcgat
     4141 tggcatgggc tagcgagggc gtcgcacccg ttagacacga ctaccgaagt cagcatgccc
     4201 actcaaccac gccctggcgg atcaagcaga aagggcggac cttgcggccc gccccttcca
     4261 actcgcagtg actgagactt ccgagaatca gaagtccata tcacccatgc cgccgccacc
     4321 accgggtggc atcatcggcg ccttcttctc cggcttctcg gcaaccatcg cctcggtggt
     4381 caccagcagg cccgcgaccg acgccgcatc ctgcagcgct gtgcgcacca ccttggtcgg
     4441 gtcgatgatg ccggacttca ccaggtcctt gaactcgccg gtctgcgcgt caaagcccca
     4501 gttgtactcg tccttgtcca gcaccttgcc ggcgatgacc gcaccgtcct cgccagcgtt
     4561 ctcggcgatc tgccgcagcg gcatcatgat cgccttgcga acgatctcga cgccgaaccg
     4621 ctgatcgtcg ttgtcggcct tcagcttcgc caggatcagc gacgcacgcg ccagcgcgac
     4681 gccaccaccc ggaacgatgc cttcctcaac cgccgcacgc gtcgcgtgca ggctgtcgtc
     4741 cacgcggtcc ttgcgctcct tcacctcgac ttcggtcgag ccgccgacgc ggatcaccgc
     4801 gacgccgccc gccagcttcg ccaggcgctc ctgcagcttc tcgcggtcgt agtccgaggt
     4861 ggtctcctcg atctgcgcac ggatctggtt gcaacggccg acgatgtcct gcttcttgcc
     4921 ggcgccctcg acgatggtgc tgttctcctt ctcgatcagc accttcttcg ccttgccgag
     4981 gtcgttcagc ttgacgttct cgagcttgat gccgagatct tcgctgatca ccgtgccgcc
     5041 ggtgaggatc gcgatgtcct cgagcatcgc cttgcggcga tcaccgaagc caggcgcctt
     5101 caccgccgcg accttcaggc cgccgcgcag cttgttcacc accagcgtcg ccagcgcctc
     5161 accctcgacg tcctcggcga tgatcagcag cggacggccg gactgcacga tgctctccag
     5221 cagcggcagc atcggctgca ggccggacag cttcttctcg tggatcagga catacggctg
     5281 atccagctcg gcgatcatct tctcggcgtt ggtgatgaag tacgggctga catagccgcg
     5341 gtcgaactgc atgccctcga ccacgtcgag ctcggtctgg atgcccttgg cttcctcgac
     5401 cgtgatcacg ccctcgttgc caaccttctg cat
//
LOCUS       DATGEM010000308         6595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1548550, whole genome shotgun sequence.
ACCESSION   DATGEM010000308 DATGEM010000000
VERSION     DATGEM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548550"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VKQ27_09865"
     CDS             <1..1506
                     /locus_tag="VKQ27_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HLY89281.1"
                     /translation="KVQQRAVRTAALRTFRHLHALSLRFHLDRQTGALARAIDRGTTG
                     IQSTLRLAVFNVVPTLIELLMVTAIIWHLFNWKFAAVTFVAVVSYVGFTMGFAGWRVR
                     ARRTMNENDNDASTKALDSLLNFETVKYFGNEAHEAARYDSALARYERAAVRVQVSLN
                     MLNIGQSLIIAAGLTLIMLMATDGVRLGQMTVGQFVLVNTYLMQLYQPLGFLGFVYMT
                     IKQGIVDMEQMFSLLEVDQEIADKPDAPDLMAHDGELRFEGVHFGYRPDREILKGIDF
                     VVPAGRKLAIVGPTGAGKSTISRVLFRFYDVTQGRITIDGQDIRDVTQESLRTTIGVV
                     PQDTVLFNDTIRYNIAYGRPDASQQEIEQAARLAQVHDFVQSLPDGYDTKVGERGLKL
                     SGGEKQRVAIARTILKNPRVLILDEATSALDTRTEQDIQAALRAVARHRTTLVIAHRL
                     STVVDADEIIVLSDGRVAERGRHSALLARGGLYARMWALQAEQETEAELAD"
     gene            1527..1754
                     /locus_tag="VKQ27_09870"
     CDS             1527..1754
                     /locus_tag="VKQ27_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89282.1"
                     /translation="MTDEQTPADLSHAGAVVDKAIEYMVGQNISSLSIASALLGGAMA
                     LLARSVADEAIIQILNNAIASVRSGELRQSH"
     gene            1813..2562
                     /locus_tag="VKQ27_09875"
     CDS             1813..2562
                     /locus_tag="VKQ27_09875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HLY89283.1"
                     /translation="MAKLEGKVALVTGSGRNIGRATVLKLAAEGAHVVVNARSNQAEA
                     DAVVREARGLGVKALAIVADVASKEQVEAMAATALSEFGRVDILINNAAIRPHKPFTE
                     LTQQDWEHVRGVVLDGAVYLTRAVIESMVKHQYGRILFFTGEGAFVGGSGRAHVSAAK
                     MGLVGFARGLASEFAPKNIRVNVVSPGSIDTQRANPEWYQGRVPNAAGIPLGRQGHVD
                     EIAATCLFLVSDDGGFITGQTIHVNGGTGYY"
     gene            2623..3606
                     /locus_tag="VKQ27_09880"
     CDS             2623..3606
                     /locus_tag="VKQ27_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HLY89284.1"
                     /translation="MPDAIPVIDVADHLAGRLGALHDLARQFRDALTTVGFMVITGHD
                     VPKTLIDQTFGEARRLHDLPIPEKLALKLNEHNNGYMAMSRYAVWTSDVNKNDKPDLN
                     EAFFIKRERGLDNPLRRSGRRFVGANVWPDEAGLPDFRAHALEYADTMDGFARRMMAP
                     LAVALDLPPDWFDEAFSESQFTLRLSHYPPVQAAANQFGIAPHTDSSFMTFLAQTEVP
                     GLQVRMESGDWLEVPYIPGSFAVNSGDMMKRWTNGRFKSTPHRALPPVGLHRYAIPFF
                     IGPQFDWEIACLPTCMGPDNPPRWPPITYAAWQEYWYDANYDPKAQQDLVA"
     gene            3640..4587
                     /locus_tag="VKQ27_09885"
     CDS             3640..4587
                     /locus_tag="VKQ27_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY89285.1"
                     /translation="MEKRKLGRSNISLPPVMFGGNVFGWTADEATSFKLLDACMDAGL
                     NAIDTADVYSVWARGHQGGESEIVIGNWLKKRGKRDDVIIATKVGSQMGSGDKGLSRA
                     WIMKEVDASLKRLQTDVIDLYQAHRDDPGTPQEETMQAFADLVKAGKVRVLGASNFTA
                     DRLKSALEVSKAHNWPRYESLQPHYNLVERQAYEAELEPVCKAEDLGVINYYALAAGF
                     LTGKYRSEADLGKSPRGGGVKKYLNERGFAVLKALDGIAASHKATPAQVALAWLIARP
                     SVTAPIASATSVDQLQDIIAAAQLQLSAAEVAALDTASV"
     gene            complement(4699..6249)
                     /locus_tag="VKQ27_09890"
     CDS             complement(4699..6249)
                     /locus_tag="VKQ27_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY89286.1"
                     /translation="MSATAAVTPLERTQHRGMITACIICATLLQSLDQTIANVALPYM
                     QGSFSASYDEITWVLTTYITAAAIMTAPVGWLAARFGRKRLFVTCIIGFTITSMLCGA
                     AQSLEQIVLFRLMQGVCSAALVPLSQAILFDIYPVEKRGFAMAVWGMGVMIGPILGPT
                     LGGYLTEMYNWRYVFYINLPFGVLAILGLILFMHESDGDRRLRFDWLGFAVLSLGIGA
                     LQMLLDRGNDQDWFSSREIIIEAVLAGLGTYLFLVHMAWAPKPLIRPVLFRDVNFSAG
                     LVLMFAVGTILVSSIALMAPWLQTLGNYPVATAGLLMAPRGIGNLFTIVLCGRLVMRV
                     DPRYLVGVGIVIQCWSFWTMTGWTPDVSVHELVTTIMIQGAGLGLVFTPLQVLAFATL
                     APSLRTEGAALFSLGRNIGAAIGVSVTTSLIAHNAQALHEVIGASVNPFNRALQMIGA
                     ANPWMNPAHHHGAAMLDQIVNHQAQIIAYINDYVLMIFTTAPALLLLFLMRRPQHAPA
                     AEMVQPNE"
     gene            complement(6313..>6595)
                     /locus_tag="VKQ27_09895"
     CDS             complement(6313..>6595)
                     /locus_tag="VKQ27_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89287.1"
                     /translation="IKTIAVGLITEPAQAEAVLQDGHADLVAMARELMIHADWPLRAA
                     KELQAGHYDLMAPAIAHRVKRRDEQSEAYPPGSAVEIPIAADRTEAYRW"
BASE COUNT         1298 a         1989 c         2152 g         1156 t
ORIGIN      
        1 aaggtgcaac agcgcgccgt gcgtaccgcc gcgctgcgca cgttccgtca tctgcatgcg
       61 ctgagcctgc gcttccatct cgatcgccag accggtgcgc tggcccgcgc gatcgaccgc
      121 ggcactacag gcatccagtc cacgctgcgg ctcgctgtat tcaacgtggt gcccacgctg
      181 atcgaactgc tgatggtcac cgcgatcatc tggcacctgt tcaactggaa gttcgccgcg
      241 gtgacatttg tcgccgtggt cagctatgtc ggcttcacga tgggcttcgc cggctggcgc
      301 gtgcgcgcgc ggcggacaat gaatgagaac gacaacgacg catccaccaa ggcgctcgac
      361 agcctgctga acttcgagac ggtgaagtat ttcggcaacg aggcgcatga ggccgcccgt
      421 tacgattcgg cgctcgcgcg ctacgagcgg gccgcggtgc gcgtgcaggt gtcgctgaac
      481 atgctgaaca ttggccaatc gttgatcatc gcggcggggc tgacactgat catgctgatg
      541 gcgaccgacg gcgtgcgcct tggccaaatg accgttggcc agttcgtgct ggtaaacacg
      601 tatctgatgc aactctatca gcctctgggc ttcctggggt tcgtctacat gacgatcaag
      661 caggggatcg tcgatatgga gcagatgttc agcctgcttg aggtggatca ggagattgcc
      721 gacaagcccg atgcgccgga cctcatggcc catgatgggg agcttcgatt cgagggcgtg
      781 cattttggtt atcgccctga tcgggagatc ctgaagggca tagattttgt cgtgccggca
      841 gggcgcaagc tggcgatcgt cgggccaacc ggcgcaggga aatcaaccat cagccgcgtg
      901 ctgttccggt tctatgacgt gacgcaaggt cgcattacga tcgatggcca ggacattcgc
      961 gacgtgacac aggagagcct gcgcaccacg atcggcgtgg tgccgcagga caccgtgctg
     1021 ttcaacgata cgatccgata caacattgcc tatggccggc ccgatgcctc gcagcaggag
     1081 atcgagcagg ccgcccggct ggcgcaggtg cacgatttcg ttcagtcact gcctgatggc
     1141 tacgacacca aggtgggcga gcgtggcctg aagctctcag gcggcgagaa gcagcgtgtg
     1201 gcgatcgcgc gcaccatcct gaaaaacccg cgcgttttga tcctggatga ggcgaccagc
     1261 gcgctggata cccgcaccga acaggacatc caggccgcgt tgcgcgccgt ggcgcggcat
     1321 cgcacgacgc tggtgattgc gcaccggctg tccacggtgg tggatgcgga cgagatcatc
     1381 gtactatcgg atggccgcgt cgccgagcgc ggcaggcact cggcgctcct ggcgcgcggt
     1441 ggtctgtatg cacgaatgtg ggcactgcaa gccgagcagg agacggaagc ggagctggcg
     1501 gattgatatg aacgggaggc gaagcaatga ctgacgaaca gacacctgcg gacctcagcc
     1561 acgccggtgc agtggtggat aaggcgatcg aatacatggt gggccagaac atcagttctc
     1621 tgtctatcgc ctcggcgctg ctaggtggag caatggcgct gttggcgcgc agcgtcgccg
     1681 atgaggcgat catccagatc ctgaacaacg ccattgccag tgtgcgcagt ggggaattgc
     1741 gccaatctca ttaggtgtca ttgcgataag cgcggcgacg aagcaatctc gcgccagcaa
     1801 ggaaggaacg tcatggccaa actcgaggga aaagttgcgc tggtcaccgg ttctggtcgg
     1861 aatatcggcc gcgccacggt gctgaagctg gcggctgaag gcgcacatgt cgtggtcaac
     1921 gcccgcagca atcaggctga ggccgacgcc gtggtgcgcg aagcccgtgg gctcggcgtg
     1981 aaggcgcttg ccatcgtggc cgacgtcgca agcaaggagc aggtcgaggc gatggccgcg
     2041 acggcgctgt cggagttcgg ccgcgttgat atcctgatca acaacgccgc catccggccg
     2101 cacaagccat tcacagaact cacccagcag gactgggaac atgtgcgtgg cgtcgtgctc
     2161 gatggtgcgg tctatctaac ccgcgccgtc atcgagtcga tggtgaaaca ccaatatgga
     2221 cgtatcctgt tcttcacggg cgaaggcgcc tttgtcggcg gcagtggacg cgcgcacgtc
     2281 tcggccgcga agatgggcct ggttggcttt gcgcgcggcc tcgccagcga gtttgcgccg
     2341 aagaacatcc gcgtgaatgt ggtttcaccc ggaagtatcg acacgcagcg ggccaatccg
     2401 gagtggtatc agggccgggt accgaatgcg gcgggcattc cgcttgggcg gcaggggcat
     2461 gtagacgaga tcgccgcgac ctgcctgttc ctggtcagcg atgatggcgg cttcatcacc
     2521 ggccagacga tccacgtgaa cggcggcacc gggtactact agggcgcatc tgttcatcgg
     2581 ggcgtccgcc tgcataatgc cgcggtgcag caggagacgc ccatgccaga cgcgatccca
     2641 gtcatcgacg ttgccgacca tctggccggc cggcttggcg cgttgcatga cctcgcgcgg
     2701 caattccgcg atgcgctcac aaccgtgggt ttcatggtga tcaccggcca cgatgtgccg
     2761 aagacgctga tcgatcagac attcggcgag gcgcgccgcc tacacgactt gccgataccg
     2821 gaaaaactcg cgctgaagct gaacgaacac aacaacggct acatggcgat gagccgctat
     2881 gcggtgtgga cctccgacgt caacaagaac gacaaaccgg acctgaacga ggcgttcttc
     2941 atcaagcgcg agcgcgggct ggacaatccg ctgcgccgct caggtcgccg ctttgttggc
     3001 gcgaatgtgt ggccggatga agcggggctg cccgatttcc gggcccacgc actcgaatat
     3061 gcagacacga tggatgggtt cgcgcgccgc atgatggcac cgttggcagt ggcgttggat
     3121 ctgccgcccg attggttcga cgaagcgttc agtgagagcc agttcaccct gcgcctgtcg
     3181 cactatccgc cggtgcaggc tgcagccaac caatttggca tcgcgccgca tacggacagc
     3241 agcttcatga cgttcctggc gcagaccgag gttcccggcc tgcaggtccg catggagtcg
     3301 ggtgactggc tcgaagtgcc ttacattccg gggtcgttcg cggttaactc cggcgacatg
     3361 atgaagcgct ggacgaacgg ccgcttcaag tcgaccccgc atcgtgcgtt gccgccggtg
     3421 ggcctgcatc gctacgccat cccgttcttc attggtccgc agttcgattg ggaaatcgcc
     3481 tgtctgccca cctgcatggg gccggacaat ccgccacgct ggccaccgat cacctacgcg
     3541 gcctggcagg aatattggta cgacgcgaac tatgatccga aggcgcagca ggatctggtg
     3601 gcgtaagtcg tgagcaccgc gcgcaaggga gagacgacaa tggaaaagcg taagctcggg
     3661 cgttccaata tttcgctgcc gccggtgatg ttcggcggca acgtgttcgg atggaccgct
     3721 gatgaggcga cctcgttcaa gctgctcgac gcgtgtatgg acgcgggcct caatgcgatt
     3781 gacacggccg atgtctattc cgtgtgggcg cgcggccatc agggaggcga gtcggaaatc
     3841 gtcattggca attggctgaa gaagcgtggc aagcgcgatg acgtgatcat cgccaccaag
     3901 gtcggcagcc agatgggctc cggcgacaag ggcctctcgc gcgcctggat catgaaagag
     3961 gttgatgcat cgctgaagcg gctccagacc gacgtgatcg atctgtatca ggcccatcgt
     4021 gacgatccgg ggacgccgca ggaagagacg atgcaggcct ttgccgatct ggtaaaggcg
     4081 ggaaaggtgc gcgtgctggg cgcctccaac ttcaccgccg atcggctgaa atcggcactc
     4141 gaggtcagca aggcgcacaa ctggccgcgc tacgagtcgc tgcaaccgca ttacaatctg
     4201 gtcgagcggc aggcctatga ggcagagctg gaaccggtat gcaaggccga ggatctcggc
     4261 gtgatcaatt actacgcctt ggcggcgggt ttcctgacgg gcaagtaccg ctccgaagcc
     4321 gatctgggta agagcccgcg cggtggtggg gtgaagaagt acctgaatga gcgtggcttc
     4381 gcggtgctga aggcgcttga cgggatcgct gcaagccaca aggcaacacc agcccaggtt
     4441 gcgctagcct ggctgatcgc gcggcccagc gtgacggcgc cgatcgcgag tgccaccagc
     4501 gtcgatcagt tgcaggacat catcgcggca gcgcagttgc agctgagcgc ggcggaggtt
     4561 gccgcgctgg atacggcgag cgtgtaggct taccgtcccc caccgtcatg ccgggcggaa
     4621 ggcccggcac ccgcgtcgtt actcgcacca gccacggcat aacgaaccga caggaccgtc
     4681 gccaagtata tcgtcccgtt actcattcgg ctgcaccatt tctgccgccg gagcgtgttg
     4741 cggccggcgc atcaggaaca ggagcagcag cgccggcgcc gtggtgaaga tcatcagcac
     4801 gtagtcgttg atataggcaa tgatctgtgc ctggtgattg acgatctgat cgagcatcgc
     4861 tgcgccgtga tggtgggccg gattcatcca tggattcgcc gcgccgatca tctgcagggc
     4921 gcgattgaac gggttgaccg atgcgccgat gacctcgtgc agcgcctggg cattgtgcgc
     4981 aatcagcgac gtcgtcacgg aaacaccgat cgccgcgccg atattgcggc ccaggctgaa
     5041 cagcgcggcg ccttccgtgc gcaggctggg cgccagcgtg gcgaatgcga gcacctggag
     5101 cggtgtaaat acaaggccga gaccggcgcc ctggatcatg atggtggtga caagctcgtg
     5161 gacggacacg tccggcgtcc agcctgtcat cgtccagaac gaccagcact ggatcacgat
     5221 gccgacgccc accaagtagc gcgggtccac ccgcatgacc agccggccgc acaggacgat
     5281 ggtaaacagg ttgccgatgc cgcgcggcgc catcagcagg ccggcggtag cgaccggata
     5341 attccccaga gtttgcaacc acggcgccat cagcgcgatc gaggacacca ggatggtgcc
     5401 gaccgcgaac atcaacacca gcccggcgga gaagttcacg tcacggaata acacaggccg
     5461 gatcaggggt ttcggcgccc aggccatgtg cacaaggaac aagtaggtgc cgaggccagc
     5521 cagtaccgcc tcgatgatga tctcgcggga ggagaaccag tcctggtcgt tgccgcgatc
     5581 cagcaacatc tggagtgcgc cgataccgag gctgagaacc gcgaaaccca accagtcaaa
     5641 gcgcagacgg cggtcgccgt ctgactcgtg catgaacagg atcagcccga ggatggccag
     5701 cacgccgaac ggcaggttaa tatagaatac gtaacgccag ttgtacatct cagtcaggta
     5761 gccaccgagc gtcgggccga ggatcgggcc gatcatcaca cccatgcccc aaacagccat
     5821 ggcaaagccg cgcttctcga ctggataaat gtcgaacagg atggcctggg acagtggcac
     5881 caaggcggca ctgcacaccc cctgcatcaa tcggaacagc acgatctgtt ccagcgactg
     5941 cgccgcgcca cacagcatcg aggtgatggt gaagccgatg atgcaggtca cgaatagccg
     6001 cttccggccg aacctcgcgg caagccagcc cactggcgcg gtcatgatcg ccgccgccgt
     6061 gatataggtg gtcagcaccc aggtgatctc gtcgtagctg gccgagaagc tgccctgcat
     6121 gtaaggcagc gccacattgg cgatcgtctg gtcgagcgat tgcagcagtg tcgcgcagat
     6181 aatgcacgcg gtgatcatgc cgcgatgttg cgtgcgctcc agcggcgtaa cggcagccgt
     6241 ggcagacatc gggcctggcg tttctccctg accgcgactc tgggcgatca gggagacagg
     6301 atcaagttac tattaccagc ggtatgcttc ggtccggtcg gcagcgatcg gaatctccac
     6361 ggcgctgccc ggcggataag cctcggactg ctcgtcccgc cgcttcaccc ggtgcgcgat
     6421 cgccggcgcc atcaggtcgt agtgtccggc ctgcaattcc tttgccgcgc gcagtggcca
     6481 gtcggcgtgg atcatcagtt cccgcgccat cgccaccaga tcggcatggc cgtcctgcag
     6541 caccgcctcg gcctgcgccg gttcggtgat caatcccacc gcgatcgtct tgatg
//
LOCUS       DATGEM010000309         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1276159, whole genome shotgun sequence.
ACCESSION   DATGEM010000309 DATGEM010000000
VERSION     DATGEM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276159"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..798)
                     /locus_tag="VKQ27_09900"
     CDS             complement(202..798)
                     /locus_tag="VKQ27_09900"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLY89288.1"
                     /translation="MLALIDNYDSFTFNLYHFLGDVGARCEVWRNDKITVADVLARAP
                     EAIVLSPGPCTPNEAGICLDLIAAAAGRIPVLGVCLGHQAIGQAFGGSVVRAPVPMHG
                     KVSRIFHTGTDIFAGVASPLSATRYHSLIVDRATMPDVLVPTAWTEDGLVMGLRHRTL
                     PIFGVQFHPESIASQHGHDLLANFLAIARGTNEPAKAA"
     gene            complement(862..1593)
                     /locus_tag="VKQ27_09905"
     CDS             complement(862..1593)
                     /locus_tag="VKQ27_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY89289.1"
                     /translation="MVSARLARATVCDPCASCEARPLSVCNAIADVDLGRLAAIATVV
                     EVSAGQCFIDEGEPARAFFNITAGTAKLFKLLPDGRRQITGFVSTGHFLGLAVSDTYA
                     FSAEAIEPVRFCRFQRSRLRSLLDDFPLMEKRLLEVAANELVAAQEQMLLLGRKTARE
                     RLASFLLLQSRQGTPCGQPRMRFIMPMTRGDIADYLGLTIETVSRTLTRLRADGMIDF
                     ASQSEVLITSRAALEHLAGGLTRIV"
     gene            1726..>2035
                     /locus_tag="VKQ27_09910"
     CDS             1726..>2035
                     /locus_tag="VKQ27_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89290.1"
                     /translation="MVDFIIETLGREQLAAVYPLVREAVPSLSLTSWLRFARPLTGSR
                     RGTHCGIVTARREGRPFPCGLFCFRVDDDLALGRVLVADHFVAVDLLDPGAVLTALVE
                     E"
BASE COUNT          342 a          568 c          752 g          373 t
ORIGIN      
        1 gtggtcgacg gcgcccggcg ggttgcattc ggtgatttaa gcggtggtcg cgagagcggg
       61 cgaagtgttc accaatttgg agcgtgatgc gtccctgcta gccaaattgg cgacgcccta
      121 tcctcattac acactccaat aaggccgaac gggattgctt cgtcgctacg cttctcgcaa
      181 tgacactacg gccgttgggc gtcacgccgc ctttgccggt tcgtttgttc cgcgggcgat
      241 cgccaggaag ttggctagca ggtcgtggcc gtgctggctg gcgatgctct cgggatggaa
      301 ctgcacaccg aagatcggca gcgtgcggtg gcggaggccc atgacgaggc cgtcctcggt
      361 ccaggcggtg ggcacgagca catcgggcat cgtggcgcgg tcgacgatga ggctgtggta
      421 gcgcgttgcg ctgagtgggg aggcgacgcc cgcgaagata tcggtgccgg tgtggaagat
      481 gcggctgacc ttgccgtgca tcggcacggg cgcgcgcacg acgctgccgc cgaaggcctg
      541 gccgatcgcc tggtggccga ggcagacgcc gagcaccggg atcctgccgg ccgcagcggc
      601 gatcaggtcg aggcagatac cggcctcatt gggcgtgcag gggccggggg agagcacgat
      661 cgcctcgggc gcgcgtgcca gaacgtctgc gacggtgatc ttgtcgttgc gccagacctc
      721 gcagcgcgcg ccgacgtcgc cgaggaagtg gtacaggttg aaggtgaatg agtcgtagtt
      781 gtcgatcagt gccagcatgg cggcaatatt tgcgccgcca tacgcggtgg gtcaattctg
      841 tgcagcaagc cgttccaggg gtcagactat cctggtcaga ccgcctgcaa gatgttccag
      901 cgcggcgcgg ctggtgatca gcacctcact ttgggaggca aagtcgatca tgccgtcggc
      961 gcgcaggcgc gtcagggtgc ggctcaccgt ttcgatggtg agaccgagat agtcggcgat
     1021 gtcgccgcgt gtcatcggca tgatgaagcg catgcggggt tgaccacacg gcgtgccttg
     1081 ccggctttgc agcagaagga atgaggcaag acgctcccga gcggtcttgc ggcctagcag
     1141 cagcatctgt tcctgcgctg ccaccaattc gttggcggcg acttcgagca ggcgcttttc
     1201 catgagggga aagtcgtcga gcagactgcg cagcctggag cgctggaagc ggcagaagcg
     1261 gaccggctcg atcgcctcgg cgctgaaggc ataggtgtcg gagacggcga ggccgaggaa
     1321 atggccggta ctgacaaagc cggtgatctg gcgccggcca tcgggcagca gcttgaaaag
     1381 cttggcggtg ccggcggtga tgttgaagaa ggcgcgcgca ggctcgcctt cgtcgatgaa
     1441 acactggccg gcggagacct cgacgacggt ggcgatggcg gcgagacgtc cgaggtccac
     1501 atcggcgatg gcattgcata cgctgagggg ccgcgcttcg cagctggcgc aggggtcgca
     1561 gacggtggca cgcgcaagcc tggccgaaac catggcgttc tccgcagggc agtattgcct
     1621 catggagtta aaattcgctt ctccttgggc cgcattgatc caggtcaacg atttgcaccc
     1681 gagccatggc gcaggctggt cgatggacgg acagggtagg cgacgatggt cgacttcatc
     1741 atcgagacgc ttgggcgcga acagcttgcg gcagtttatc cgttggtacg ggaagctgtg
     1801 ccaagtttga gcctgacaag ctggctgcgg ttcgcccggc cgctgaccgg gtcgcggcgt
     1861 ggcacgcatt gcgggatcgt gacggcgcgg cgcgaggggc ggccgttccc gtgtgggctg
     1921 ttctgcttcc gggtggatga tgacctggca ttgggtcggg tgctggtggc ggatcatttc
     1981 gtcgcagtgg acctgctgga tcccggggca gtgctgacgg cgctggtcga ggaac
//
LOCUS       DATGEM010000310         8049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1213709, whole genome shotgun sequence.
ACCESSION   DATGEM010000310 DATGEM010000000
VERSION     DATGEM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213709"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VKQ27_09915"
     CDS             <1..131
                     /locus_tag="VKQ27_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HLY89291.1"
                     /translation="AGKAAGTSIVLKDYVRFQLGEGIEKEESDFAAEVAAAAGVKA"
     gene            296..1018
                     /gene="pyrH"
                     /locus_tag="VKQ27_09920"
     CDS             296..1018
                     /gene="pyrH"
                     /locus_tag="VKQ27_09920"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLY89292.1"
                     /translation="MTRPRFQRILLKLSGEVLMGSGQFGIDPDTVARVAGEIAAAKEA
                     GHELCIVVGGGNIFRGLAAAEVGFERATADYMGMLATVMNALAVQNALEQIGVDTRVQ
                     SAIPMASVCEPFIRRRAERHLEKGRVVIFAAGVGSPFFTTDSGAALRAAEMKCDALFK
                     GTSVDGVYNADPKKVADAQRYEEVSFSRVLSDDLKVMDASAIALCRDNNIPIVVFNIR
                     EQGNLAKVLAGEGTATIVRNGE"
     gene            1020..1583
                     /gene="frr"
                     /locus_tag="VKQ27_09925"
     CDS             1020..1583
                     /gene="frr"
                     /locus_tag="VKQ27_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLY89293.1"
                     /translation="MAGPAYDKADLERRMAGAVESLKHDLQGLRTGRASTALLDPVTV
                     EVYGSHMPLNQLATVSAPEPRMLSVQVWDKSNVGPADKAIRSAGLGLNPIVDGQTLRL
                     PIPDLTEERRKELAKLCGSYAEKARIAVRNVRRDGMDDLKLDEKKNGLSEDERKRHEG
                     EVQKLTDATIVEIDAATAAKEKEILGK"
     gene            1613..2335
                     /locus_tag="VKQ27_09930"
     CDS             1613..2335
                     /locus_tag="VKQ27_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HLY89294.1"
                     /translation="MGSGGPDDPVVPRHVAIIMDGNGRWAKARRLPRVAGHKQGIEAV
                     RRTVRAAGDLGIEALTLYAFSSENWRRPAAEIADLMGLLRHYIRAELNELARQGVRLR
                     IVGDYRKLEPDLVRMLDEAVEKTAGNSRLTLAIALNYGSQDELLRATRRLMREARAGE
                     MDPDALDDAAFEARLDTAGLPRLDLLIRTSGEQRLSNFLLWQAAYAELVFTDTLWPDF
                     DREHLAAAIDSFGRRERRYGGL"
     gene            2332..3126
                     /locus_tag="VKQ27_09935"
     CDS             2332..3126
                     /locus_tag="VKQ27_09935"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLY89295.1"
                     /translation="MTAATTAAAGRCSDLAIRSVTGVTLILIAVACLVYGQDPFWLLL
                     ATATLVMMAEWASLIHAERWQMWLAFVGLTLALFLEQPHLDIPTAAAWYALGTATLCV
                     AVVTRNIRLGAGMLYAGLPVLSLFYIHEQYDGIDLSFWTLAVVWATDIGAYFSGRAIG
                     GPKLAPRWSPNKTWAGLIGGMISATLVGFGLLTVLHVQPRLAAFGGLLAVAAQAGDLF
                     ESMMKRRAGVKDSGWLLPGHGGVMDRLDGVVPVVCIVALIVASGIL"
     gene            3131..4285
                     /locus_tag="VKQ27_09940"
     CDS             3131..4285
                     /locus_tag="VKQ27_09940"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLY89296.1"
                     /translation="MRTVTILGATGSVGRSTLDLVEREPDRFHVEALTAQSDVEGLAA
                     AALRTGAAVAVIGDASCYGALKDALAGSSVEAAAGPAAIVEAAGRGADWTMASIVGLA
                     GLEPTMAALSCGKTVALANKESLVSAGSIMIRAAEAAGARLLPVDSEHNAVFQCYEPQ
                     PGRVSRIILTASGGPFRTWTHDQMRTVTPAQAVKHPNWSMGAKISVDSATMMNKGLEL
                     IEAHHLFPVGADKLDVLVHPQSVVHSMVEYIDGSVLAQLGAPDMRIPIAYALAWPERM
                     ETPCARLDLAAVGKLEFEAPDYVRFPALALAKAALTEGGARPAILNAANESAVAAFLG
                     GQIGFLDIAAVVEQVLAQYEPPPPDRLADVVAIDAEARQKAAQVMQRMAA"
     gene            4282..5415
                     /locus_tag="VKQ27_09945"
     CDS             4282..5415
                     /locus_tag="VKQ27_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HLY89297.1"
                     /translation="MTEIGGFVWAVACFLLVIGPLIFIHELGHYFVGRWFGVKAETFS
                     IGFGREVFGWTDRRGTRWKVAALPLGGYVRFAGDMGPASLPTPEWLALPATERVQTFQ
                     AKPVWQRFLIVLAGPAVNFIFALLVIAAILMAFGEPVTPTVVATVQPASGAAAAGIHP
                     EDHILRIDGVPMRRFEDIAGYTFLHPEKDLRIDLLRSGRPLTLVAHTHAAIEEDRFGN
                     RARKGVLGISPHPGAIQPVPLAEVPVRAGGYVIQAVDTTLTAISQIILGTRSASELSG
                     PVGVARTAGEQATLGWFPIVLLMAGISINLGFINLLPIPMLDGGHLLFYLVEAVRRRP
                     VAPQVQEWAFRSGLALLLGLMLFVTINDLGTAGLWQRLVRLIG"
     gene            5547..>8049
                     /gene="bamA"
                     /locus_tag="VKQ27_09950"
     CDS             5547..>8049
                     /gene="bamA"
                     /locus_tag="VKQ27_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HLY89298.1"
                     /translation="MEGKYSRIGSALLIGTILGTGYVVPAQARREMAKPHGVPVPTAP
                     TAPSASAPAPASAQEIIRSIKVTGNQRLESETVLSYIKLRVGEVYTRESGDTALKALF
                     ATELFADVTIRDENAGDIVVAVRENPVVNRIVLEGNKRLKSDKIMPEIKLSAREIFTR
                     SKVRADVGRIIELYRRQGRYAASVEPQMVLLDQNRVDVVFEIHEGEKSKVRRINIIGN
                     EHFSDSQIKAQMATKEATFTHFLSSGTSYDPDRLAYDQQKLRQFYLTQGYADFRVVSA
                     VAELTPDKKDFIVTYVVEEGKRYKFGKINVESDIRDLKPAMLQPLVRVKPGDWYNAKT
                     VEDTVDSMTETAGLLGYAFASIEPNFNRDGNKLTMGVTFHVNQSPRVYVERIDINGNT
                     LTQDKVVRREFRLAEGDAFNGYQVKRSRDRIRSLGFFQDKLEIDQKPGSAPDKVVLEA
                     NVQEKSTGQLQISAGYSSLEKFIVNLSIEQANFRGKGQTVRASADWSAYSKSVSLGFT
                     EPYLFDKNIALGGDIFRRDYRSFDYTNNGNDRNTTYNQTTTGFQIRMGIPLTEYWSLA
                     TRYGLSQDKVSLDKNTYYSTFNSTGTLACDPILAGRYLCDAVGNRTTSSVGYSLAFDN
                     LNNRITPTAGNRFVFSQDFAGLGGSVKYVRTTAQDDKYHNLGAGFVLNIHGEGGYILP
                     YGSKRFDDTGNEVDRIRLTDRFYLGEPQIRGFDIRGVGPRVVRYSYNADGTPNLTKDG
                     KLQDDALGGRAYYLAHLEMQIPLGSGAKEAGFKPSIFMDAGSVFGVKKPQTDDFKPGD
                     PALTRPILDASGNHQCIDTSGALSTKPSTGCATGTS"
BASE COUNT         1447 a         2419 c         2651 g         1532 t
ORIGIN      
        1 aggccgggaa ggccgccggc acctcgatcg ttctgaagga ctatgtccgc ttccagctcg
       61 gtgaaggcat cgagaaggaa gagagcgact tcgccgctga agttgctgct gcggccggcg
      121 ttaaggccta agcttcgctg agtggcgccc gtcggcgcgc gcggttcgga aacgggcgcg
      181 cgagcaggcg ggcgttttct tttcgtggcg gatggtggtt caccctgctt ggcaggggcg
      241 ggggccatct ctaaggtgcc gcgactttct cgatccgatc ggacatcatt cctgcatgac
      301 ccgcccgcgt tttcaacgaa tcctgctcaa gctctcgggc gaggtgctga tgggctccgg
      361 ccagttcggc atcgatccgg acacggtcgc ccgcgttgcc ggtgaaatcg ctgctgccaa
      421 ggaagcgggg cacgagctct gcatcgtggt gggtggcggc aatatcttcc gcggcctcgc
      481 tgctgccgaa gtgggcttcg agcgcgccac cgccgattat atgggcatgc tcgcgaccgt
      541 catgaacgcg cttgccgtgc agaatgcgct cgagcagatc ggcgtcgata cgcgcgtaca
      601 gtcggccatt ccgatggctt cggtgtgcga acccttcatc cgccgccgtg cagagcgtca
      661 cctggaaaag gggcgcgtag tgatcttcgc cgccggcgtg ggcagcccgt tcttcaccac
      721 cgatagcggc gctgcgctgc gcgccgccga gatgaagtgc gacgcattgt tcaagggcac
      781 ttcggtcgat ggcgtttata atgccgatcc gaagaaggtg gccgatgccc agcgttacga
      841 ggaagtcagc ttcagccgcg tgttgagcga cgacctcaag gtgatggatg ccagcgcgat
      901 tgcgctgtgc cgtgacaata acatcccgat cgtcgtcttc aatatccgcg agcaaggcaa
      961 tttggccaaa gtgctcgccg gcgaaggcac ggcgacgatc gtccgcaacg gagagtgaga
     1021 tggcgggacc cgcttacgac aaggccgacc tcgagcgccg catggccggc gcggttgaat
     1081 cgctgaagca cgaccttcag gggctgcgca cggggcgcgc ctcgacggcg ctgctcgatc
     1141 cggtgacggt tgaggtttac ggctctcaca tgccgctcaa ccagcttgcg accgtctcgg
     1201 cacccgagcc gcgcatgctg tcggtgcagg tgtgggacaa gtcgaatgtc gggccggccg
     1261 acaaggcgat ccgctcggcg ggcctcggcc tcaacccgat cgtcgatggc cagacgctgc
     1321 gcctgccgat ccccgacctc acggaagagc gccgcaagga gcttgccaag ctgtgtggct
     1381 cctacgccga aaaggcccgg atcgccgtgc gcaacgtgcg tcgtgacggc atggatgatc
     1441 tcaagctcga cgagaagaag aacggcctct ccgaggacga gcgtaagcgc cacgaaggcg
     1501 aagtgcagaa gctgactgac gccaccattg tcgagattga tgcggcgacg gcggcgaagg
     1561 agaaggaaat cctcggcaag tgacgctcgc gtccgccaat gccgcatcca ccgtggggag
     1621 cggtggcccg gacgatcctg tggtaccgcg tcatgtcgcg atcatcatgg acggcaatgg
     1681 ccgctgggcg aaggcccgtc ggctgccgcg cgttgctggc cacaagcaag gcatcgaggc
     1741 cgtacgccgt accgttcgcg cggcgggcga cctgggcatc gaggcgctga cgctctacgc
     1801 cttctcgtcg gagaactggc gccgtccggc tgccgagatc gccgatctga tggggctact
     1861 gcgccactat atccgcgcgg agctcaacga gcttgcgcgc cagggtgtgc gactgcggat
     1921 cgtcggcgac tatcgcaagc tcgagccaga cctcgttcgc atgctcgacg aggcggtgga
     1981 gaagacggcc ggcaatagcc ggctgaccct cgcgatcgcc ctcaactatg gctcgcagga
     2041 cgaattgctg cgcgcgacgc gccggctgat gcgtgaggcc cgtgccggtg agatggatcc
     2101 cgatgcactg gacgatgccg cgttcgaggc gcggctcgat accgcaggcc tcccccggct
     2161 ggatctgctg atccggacgt cgggcgaaca acgcctctcc aatttcctgc tatggcaggc
     2221 cgcatacgcc gagcttgtct tcaccgacac actctggccg gatttcgacc gcgagcatct
     2281 cgccgccgcc atcgacagtt tcggccggcg cgagcggcgt tacggggggc tatgacagcc
     2341 gccacgaccg cagcggctgg ccgctgctcc gatctcgcta tccgctcagt caccggggtc
     2401 acgctgatcc tgatcgcagt cgcgtgtctg gtctacggcc aggatccgtt ctggttgctg
     2461 ctggcgacgg ccacgctggt gatgatggcg gaatgggcga gcctgatcca cgcagagcgt
     2521 tggcagatgt ggctcgcgtt cgtggggctc acgctcgcgc tgtttctcga gcagccccac
     2581 ctcgacatac ccacggcggc tgcttggtat gctctgggaa ccgcgacgct ctgtgttgca
     2641 gtcgtcaccc gcaacattcg gctcggggct ggcatgctct atgccggtct tccagtccta
     2701 tcgcttttct acattcacga gcaatatgac ggcattgacc tgagcttttg gacgctcgcg
     2761 gtggtatggg cgacggatat cggagcgtat ttctccggac gggcgattgg cgggcccaag
     2821 ctcgcgccgc ggtggagccc caacaagacc tgggcggggc tgatcggtgg tatgatttcg
     2881 gccacgctgg ttgggtttgg cttgttgacg gtgctgcatg tgcagccgcg gctggctgcg
     2941 ttcggcgggt tgcttgctgt cgcggcgcaa gcaggcgatc tgttcgagag catgatgaag
     3001 cgtcgcgcag gcgtgaagga ttcgggctgg ctgttgcccg gccatggcgg cgtgatggac
     3061 aggctggacg gtgtcgtccc agtggtctgc atcgtcgcgc tgatcgtcgc atcagggata
     3121 ttgtgaaacc atgcggactg ttaccattct gggcgccaca ggttcggtcg ggcgttcgac
     3181 gctcgatctg gtcgaacggg agcccgaccg ttttcatgtc gaggcgctga ccgcgcagag
     3241 cgatgtggag ggactggccg ccgcggccct gcggaccggt gcggccgtgg ccgtgatcgg
     3301 cgacgcaagc tgctatgggg cgctcaagga tgcgcttgca ggaagcagcg tcgaggcggc
     3361 cgcaggaccg gctgcgatcg tggaagccgc agggcgcgga gcggactgga cgatggcttc
     3421 catcgtgggc cttgccgggc tggagccgac gatggcggca ttgtcgtgcg gcaagacggt
     3481 cgcactggcg aacaaggaat cgctcgtctc agccggctcg atcatgattc gcgcagccga
     3541 agctgcggga gcgcgattgc tgcccgtcga ctcggagcac aacgccgtct tccaatgcta
     3601 cgagccgcag cccggtcgcg tcagccggat cattctcacg gccagcggtg gtccattccg
     3661 cacctggacg catgatcaga tgcgcacggt gacgcctgca caggcggtga aacacccgaa
     3721 ctggtcgatg ggcgcgaaga tctcggtcga ttcggcgacg atgatgaaca agggcctcga
     3781 actgatcgag gcgcatcacc tcttcccggt tggggccgac aagctggacg tgcttgtcca
     3841 tccccagtcg gtcgtccact cgatggtcga atatatcgac gggtcggttc tggcgcagct
     3901 tggtgcacca gacatgcgga ttccgatcgc ttacgcgctg gcatggcccg agcgcatgga
     3961 gacgccctgc gcccggctcg acctcgcggc tgtcggcaag ctggagttcg aggcgcccga
     4021 ttatgttcgt tttccggcgc tggcgctcgc caaggcggcg ctgacggaag ggggcgcgcg
     4081 gcctgcaatt ctcaatgccg caaatgaatc agccgtggcg gcgttcctgg gcgggcagat
     4141 cggttttctg gatatcgcgg ctgtcgtgga acaggttctg gcacaatatg agccacctcc
     4201 gcccgatagg cttgccgatg tcgtggcgat cgatgccgag gcacgtcaaa aagctgcaca
     4261 ggtgatgcag aggatggcgg catgacggag atcggcggct ttgtctgggc ggtagcatgt
     4321 ttcctgctcg tgatcgggcc gctgatcttt atccacgaac tcggtcatta tttcgtcggg
     4381 cgctggttcg gggtgaaggc ggagaccttc tccatcgggt tcggcaggga ggtattcggc
     4441 tggaccgacc gacgcggaac ccgatggaag gtcgcagctc tgccgctcgg gggctatgtt
     4501 cgcttcgccg gcgatatggg gccggcgagc ctgcccacgc cggaatggct ggcacttcca
     4561 gccacggagc gtgtgcagac cttccaggca aagccggtat ggcagcgttt cctgatcgtg
     4621 ctggctgggc cagccgtgaa cttcattttc gcgctcctgg tgatcgcagc catcctgatg
     4681 gctttcggcg agcccgtgac accgaccgtc gtggccacag tacaacccgc atcgggcgcg
     4741 gctgccgccg gtattcatcc tgaagaccat atcctgcgca tcgatggggt tccgatgcgc
     4801 cgcttcgagg acatcgcggg ctacacattc ctgcatccgg agaaggatct gcgaatcgat
     4861 ctgctgagga gcggacggcc tctcacgctc gtcgcccata cgcacgcggc gatcgaggag
     4921 gatcgtttcg gtaaccgggc ccggaagggg gtgttgggca tttcacccca tccaggcgcg
     4981 atccagcctg tgccgttagc cgaggttccg gtgcgcgccg gtggctatgt catccaggcg
     5041 gtcgatacga cgttgaccgc gatctcacag atcatcctcg gaacacggtc tgccagcgag
     5101 ttgtcaggcc cagtaggtgt tgcgcgaacc gctggtgagc aagcgaccct cggctggttt
     5161 ccgatcgtcc tgttgatggc gggtatttcg atcaatctgg ggttcatcaa tctcctgcca
     5221 attccgatgc tcgatggcgg acatcttctc ttctatctgg tcgaggcggt ccggcggcgt
     5281 ccagtcgcgc cgcaggttca ggaatgggcg tttcgatcgg ggcttgcgct tttgctcggc
     5341 cttatgctct tcgtgacgat caatgacctg ggaacggcgg gcctctggca gaggctggta
     5401 agactgatcg gctgaggccg gacatcggcg gaccggagag agccgggttg atcgccgaag
     5461 cttgatcggg caggggcgta ggacagggag cgcgggcatg gtgcaatacc atgtctggca
     5521 agtgcgaggg cgggtacagc gtgagaatgg aaggcaagta cagccggatt ggatccgcgc
     5581 tgctgattgg aacgatcctc ggtacgggtt atgtcgtccc cgcccaggcc cgccgcgaaa
     5641 tggccaagcc gcacggtgtt ccggtgccga cggctccgac tgcgccgagc gcgtcggctc
     5701 ctgcgccggc cagtgctcag gaaatcatcc gctcgatcaa ggtgacgggc aaccagcgtc
     5761 tcgaatctga gacggtgctc tcgtatatca agctgcgggt cggcgaagtt tacacgcgcg
     5821 agtcaggcga cacggcgctg aaggcgcttt tcgcgaccga actctttgcg gacgtcacca
     5881 ttcgtgacga gaatgccggc gatatcgtcg tcgcggtgcg cgaaaacccg gtcgtcaatc
     5941 ggatcgtgct cgagggcaac aagcgcctca agtccgacaa gatcatgccg gagatcaagc
     6001 tctccgctcg tgagatcttc acccgctcga aggttcgtgc cgacgtcggc cggatcatcg
     6061 agctctaccg ccgccaggga cgctatgccg ccagcgtcga gccgcagatg gtgttgctcg
     6121 accagaatcg cgtcgacgtc gtgttcgaga tccacgaagg cgagaagtcc aaggtccgcc
     6181 ggatcaacat catcggcaac gagcatttct cggatagcca gatcaaggcg cagatggcga
     6241 cgaaggaagc gacgttcacg cacttcctgt cgtccggcac cagctatgat cccgatcgcc
     6301 tggcctatga tcagcagaag cttcgccagt tctacctgac gcagggttat gctgacttcc
     6361 gcgtggtgtc cgccgttgcc gagcttacgc cggacaagaa ggacttcatc gtcacctacg
     6421 tggtcgagga agggaagcgc tacaagttcg gcaagatcaa cgttgaaagc gacatccgcg
     6481 atctcaagcc ggcgatgttg cagccgctgg ttcgcgtgaa accgggggac tggtacaacg
     6541 cgaagaccgt cgaggacacg gtcgactcta tgacggagac ggccggcctg ctgggctacg
     6601 ccttcgccag catagagccg aacttcaatc gcgacggcaa caagctgacc atgggggtga
     6661 ccttccatgt gaaccagtcg ccccgcgtct acgtcgaacg catcgacatc aacggcaaca
     6721 cgctcacgca ggacaaggtc gtccggcgcg agttccgcct cgccgagggg gatgcgttca
     6781 acggttatca ggtcaagcgc tcgcgggatc gtattcggtc gctgggtttc tttcaggaca
     6841 agctggagat cgatcagaag cccggctcgg caccggacaa ggtggtgctc gaagcgaacg
     6901 tgcaggagaa gtcgaccggt cagcttcaga tctcggccgg ctattcgagc cttgagaagt
     6961 tcatcgtcaa cctgtcgatc gaacaggcca acttccgcgg caaggggcag accgttcgcg
     7021 ccagcgcgga ctggtcggcc tattccaagt cggttagcct aggcttcacc gagccctatc
     7081 tgttcgacaa gaatatcgcg cttggtggtg acatcttccg tcgcgactat cgatcgttcg
     7141 actatacaaa caacggcaac gatcgaaaca cgacctacaa ccagacgacg accggtttcc
     7201 agattcgcat gggcatcccg ctcaccgaat actggtcgtt ggcaacgcgc tatggcctca
     7261 gtcaggacaa ggtgtcgctc gacaagaaca cctattacag cacgttcaac tccacgggca
     7321 cgttggcttg cgatccgatc ctggccggcc gctatctctg cgacgctgtc ggcaaccgga
     7381 cgacgtcctc ggtcggctac agcctcgcgt tcgacaatct caacaaccgg atcacgccga
     7441 cggcgggcaa ccggtttgtg ttcagccagg atttcgcggg tctgggcggc agcgtgaaat
     7501 atgtccgcac gaccgcgcag gatgataaat atcataacct cggtgccggt ttcgttctga
     7561 atattcacgg cgagggcggg tatatcctgc cctatggctc gaagcgcttc gacgatacgg
     7621 gtaacgaggt cgatcgcatc cgcttgacgg atcgcttcta tctgggtgag ccgcagattc
     7681 gtggcttcga catccgaggg gttggcccgc gtgtcgttcg ttattcgtac aatgcggatg
     7741 gtacgcccaa cctgacgaag gatggcaagc tacaggacga tgcgctgggc ggtcgtgcct
     7801 attatctcgc ccacctcgaa atgcagattc cgctcggttc cggcgccaag gaagcgggtt
     7861 tcaagccctc gatcttcatg gacgcaggtt cagtattcgg ggtgaagaag ccacagacgg
     7921 atgattttaa gccaggagat cccgcgctga cgcggccgat cctcgatgcc agcggcaacc
     7981 accagtgcat cgatacgagc ggcgcgctgt cgaccaaacc gtcgacgggc tgcgccacgg
     8041 gtacgtcgg
//
LOCUS       DATGEM010000311         7962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_310599, whole genome shotgun sequence.
ACCESSION   DATGEM010000311 DATGEM010000000
VERSION     DATGEM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310599"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VKQ27_09955"
     CDS             <1..1459
                     /locus_tag="VKQ27_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89299.1"
                     /translation="AASPDNAALTAIAALLAHRSDEAGGLDDRRLPDTDDIELWRAYR
                     QVQLQPRSPSAASQFAATLPLLLSYPPEMRQRLLPVVAEALVEGGEIDAATALLRTRK
                     DDGSLDLARGMLEQAKGDIDAALATYDRLAQSRDQRDHARAAVNAVNLRFAAGRIDAR
                     EAAERLDKLQYAWRGDRFEEELRDRLVALRQQSGDWRAALALLRENVALSPDNPAPRA
                     RLTDAFAAFLRGSAADSMTPLEFVSLVNENADLLPAGPDGDALEARLADRLLALDLPQ
                     RAGPVLDKLMKAAPTGAGRAVFGARLAAVRQREGDARGVLDALSASDAPDLPKELTDR
                     RTMLLATAQALGGDTQRALSELATVDGPAADEARAAILERANNWPAAQKALADYVAKT
                     VPPEGALGETQRRTLLRLATAAARAGDDASLATLRQTHTARMGSGPLADMFRLLTADK
                     VRDVSDLKRSGQEAALARALPAELKALQQGGAQTR"
     gene            1588..2016
                     /gene="speD"
                     /locus_tag="VKQ27_09960"
     CDS             1588..2016
                     /gene="speD"
                     /locus_tag="VKQ27_09960"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HLY89300.1"
                     /translation="MDALSPLGMVSDFPSGIDQKDYFVEKDGVKFAGTHLLLDLWGAR
                     SLTDPELIDRALRNAAEAANATILHSHFHHFGPDGGVSGVLVLAESHISIHTWPERAF
                     AAVDIFMCGACNPYHSLPVLKEVFAPKSILLSEQRRGLIP"
     gene            2017..2886
                     /gene="speE"
                     /locus_tag="VKQ27_09965"
     CDS             2017..2886
                     /gene="speE"
                     /locus_tag="VKQ27_09965"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HLY89301.1"
                     /translation="MTTDSWLNETLYPDWGQRFRVKRELARVQSDFQDIVIFESFSHG
                     RVMVLDGVIQITERDEFVYQEMLTHVPLLAHGAAANVLIIGAGDGGVLRRVLQHRGVQ
                     RAVMAEIDGEVIRLAREHMPDIGGGAWTDPRAEVIVGDGIDYVKRAPDASCDVIIVDS
                     TDPIGVGEVLFTDEFYANCARILTARGLVVNQCGVPFMQADELRDTSARRAKFFPHVT
                     AYVAAVPTYVGGFMTLGWAAKDPSLTRVGVDDVQARADAAGILGTTRYWTPEIHVGAF
                     SLPPYIGEYLPRG"
     gene            complement(2897..3619)
                     /locus_tag="VKQ27_09970"
     CDS             complement(2897..3619)
                     /locus_tag="VKQ27_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89302.1"
                     /translation="MKVLAIWLLLIGSAIAQERVDVQSRPGVTQPVYITAAANPRATV
                     LLFPGAAGVYEHSRNNFLVRVAPQFSAAGMTVAVFDTPSDHPNGMGQPFRASPQHGTD
                     IAAVVALLKSRSPAPLWLVGTSNGSISAAEGAAVVGRPGIAGTVLTSSVWQGGMLSTP
                     LGQIRMPVLVVHNHDDGCRFSPFSDTASSMALMTQASPKELLAVSGGSQRSDPCQALS
                     PHGYYGIENQVVPSIIAWIQVH"
     gene            complement(3616..4065)
                     /locus_tag="VKQ27_09975"
     CDS             complement(3616..4065)
                     /locus_tag="VKQ27_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLY89303.1"
                     /translation="MARISVQQADFDVGIEFAALTAGRTDIGGIGCFVGTVRNNPGEH
                     VVAMTLEHYPAMTERAIARIVEEAERRWSLLGCTVIHRVGRLTPGANIVLVLAASSHR
                     QAALDATAFLIDWLKTKAPFWKREELTDGAATWVEAREADDAAAARW"
     gene            complement(4070..4342)
                     /gene="moaD"
                     /locus_tag="VKQ27_09980"
                     /pseudo
     CDS             complement(4070..4342)
                     /gene="moaD"
                     /locus_tag="VKQ27_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
     gene            complement(4349..4966)
                     /locus_tag="VKQ27_09985"
     CDS             complement(4349..4966)
                     /locus_tag="VKQ27_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89304.1"
                     /translation="MRRIAFAFLCLTLSGCAYNTWWNPPFSSGSNPNMPAGSSENMRR
                     VVGEQVDVKPLTPEPGDIWPGPLPPEPTLQELEQQGEQAAPEQPVPGSPQFEQQQKGA
                     PPYQPPPPAGRGSSTPPGSAQPGLSALPLPKSPPPASITPPPRNPAGQVYQTPGGPVV
                     TNGGTSGYQTTTTPGGGSAIIVPNGNGTSTIIHSDGRIETVPTPR"
     gene            complement(5055..5540)
                     /gene="mobB"
                     /locus_tag="VKQ27_09990"
     CDS             complement(5055..5540)
                     /gene="mobB"
                     /locus_tag="VKQ27_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HLY89305.1"
                     /translation="MLGIVGWSGSGKTTLLTRLIPLLRARGLTISTVKHTHHGFDMDK
                     PGKDTYRHREAGAHEVLVASGTRWALLHEVVGREPDLPVLLERMEPVDLVLVEGYKTH
                     PFPKLEVYRPAVGKPPIWPEQPDIVAVASDILVETDRIMLPLNDPAPIAAWIIEFCIA
                     A"
     gene            complement(5549..6154)
                     /gene="pgsA"
                     /locus_tag="VKQ27_09995"
     CDS             complement(5549..6154)
                     /gene="pgsA"
                     /locus_tag="VKQ27_09995"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLY89306.1"
                     /translation="MLTDLPNLLTLSRIAAIPLLVVLVALHQAPADIGACIVFSAAAI
                     TDFFDGRLARSRSQQSDLGRMLDPIADKLLVAAALMMLVGLGRLSALGLYPAIVIMLR
                     EILVSGLREYLAELRIGLPVTQLAKWKTGFQMGALGTLLAGDSSASWLHLSFIPVTGI
                     GEIMLWVAAVLTLWTGWDYLMAGLHHASVPRSPVMPKRQVP"
     gene            6367..6805
                     /locus_tag="VKQ27_10000"
                     /pseudo
     CDS             6367..6805
                     /locus_tag="VKQ27_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089859.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            6928..7527
                     /locus_tag="VKQ27_10005"
     CDS             6928..7527
                     /locus_tag="VKQ27_10005"
                     /inference="COORDINATES: protein motif:HMM:NF028177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HLY89307.1"
                     /translation="MTSVRPSDLQSFLTLAETAILRGTRDDGPVPQAAERVFSALRAS
                     VGQVGQAAPARPPVCRHLTSALEQARRAPGPVGALAAAFAAVEPRLEWKARAGAEAHG
                     EAFLAGHANAALIGSEGLEIRGDVWIGVSLMAPGTRYPDHRHPPEEIYVVLSPGQWRQ
                     ADKPWHEPGIGGLVYNPPDIVHAMRSMGEPLLALWFLPT"
     gene            complement(7537..>7962)
                     /locus_tag="VKQ27_10010"
     CDS             complement(7537..>7962)
                     /locus_tag="VKQ27_10010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY89308.1"
                     /translation="DRQIAVNLRTTLELLQALVPLMAERGWGRVVTIGSVQQERPHPS
                     MFVYAGTKAAQFNWALSLARQFGGQGVTVNNLAPGAILTARNRAQMAVEGDALVKHIP
                     TGRLGRPDDLAGAAMLLCSDAGSYINGANLFVDGGRTAI"
BASE COUNT         1345 a         2410 c         2792 g         1415 t
ORIGIN      
        1 ggcggcctca ccggacaacg ccgccctcac tgcgattgcg gcgctgctcg cgcatcgttc
       61 ggacgaggcc gggggccttg acgatcgccg cttgcccgat accgacgaca tcgaactctg
      121 gcgggcctat cgtcaggtgc aactgcagcc ccgttcgcca tccgctgcct cccaattcgc
      181 ggcgacattg ccactcctgt tgagctatcc gccggaaatg cgccagcgct tgctaccggt
      241 ggtggccgag gctctggtgg agggcggcga gatcgatgca gccaccgcgc tgctgaggac
      301 acgcaaggat gacggatcgc tcgatctggc gcgcggcatg ttggaacagg cgaaaggcga
      361 catcgacgca gctctggcga cctatgatcg ccttgcgcaa tcccgggatc agcgggacca
      421 tgcccgtgcg gcggtgaacg ccgtcaattt gcggtttgcg gccggacgca tcgacgcgcg
      481 cgaggccgcg gagcggctcg acaagttgca gtatgcatgg cgcggcgatc gcttcgaaga
      541 ggagttgcgc gatcgcctgg tggccttacg ccagcaatcg ggtgactggc gggcagcgct
      601 tgccctgctg cgcgagaacg tggctctgtc gcctgacaat ccagctccgc gcgccaggct
      661 gaccgacgca tttgccgcct tcctacgagg cagcgctgcc gattcaatga ccccgctgga
      721 gttcgtttcg ttggtgaatg agaatgccga cttgctgccc gctggaccgg atggtgatgc
      781 gctagaggcg cggctggccg atcggctgct ggctctcgac ctgccgcagc gtgccggtcc
      841 ggtgttggac aagctaatga aagcggcgcc gacgggtgcc gggcgcgccg tgtttggcgc
      901 ccgtctggcg gcggtgcggc agagggaggg cgacgcccgt ggtgtgctcg atgcattatc
      961 ggcgtccgac gcaccggacc ttccgaagga gctgacggat cggcgcacga tgcttctagc
     1021 gaccgcgcag gcgctgggtg gcgacacgca gcgtgccttg agcgaacttg ccacggtaga
     1081 cggtcccgcg gcggacgagg cacgcgctgc gatcctggag cgggccaaca actggccggc
     1141 cgcgcagaag gctttggcgg attacgtcgc caaaacggtg ccgccggagg gcgcactggg
     1201 cgagacgcag cggcggacgc tgctgcggct ggcgaccgcg gcggcgcggg ctggcgatga
     1261 tgcgtcgctc gctactctgc ggcagaccca caccgcgcgt atgggtagtg gtccgctagc
     1321 ggacatgttc aggctgctga cggcggacaa ggtgcgggat gtgtcggatc tcaagcgatc
     1381 tggccaggag gcggcccttg cccgcgcgtt gccggccgag ctgaaagccc tacagcaggg
     1441 aggtgcgcag acccgctagg cagatgatca aaaaaggggg ttgcatcacg cccctccttt
     1501 gattatttgc cgcgcccttg gcccgaggga acggacgtgg cgacgcgccc gttcaacagg
     1561 ctgtctgctg gtaggaacgg ggtagcgatg gacgcactca gcccactggg gatggtctcg
     1621 gactttccga gcggcatcga tcagaaggac tatttcgtcg agaaggacgg cgtgaagttc
     1681 gccggtacgc atctgctgct ggatttgtgg ggggcgcgca gcctcacgga tccggagttg
     1741 atcgaccgtg cgttgcgtaa tgccgcggaa gcagccaacg cgacaatatt gcacagccac
     1801 ttccatcatt tcgggccgga tggcggcgtc agcggcgtgc tggtgctggc ggagagccac
     1861 atctcgatcc acacatggcc agagcgcgcc ttcgccgcgg tggatatctt catgtgcggc
     1921 gcgtgtaatc cctatcactc gctgccggtg ctgaaggagg tgttcgcgcc gaaatcgatc
     1981 ctgctgtccg agcagcggcg cgggctgatc ccgtaagtga ccaccgatag ctggctcaac
     2041 gagacgctgt atcctgactg ggggcagcgc ttccgggtga agcgtgagct cgcgcgtgtg
     2101 cagagcgact tccaggacat cgtgatcttc gagagtttct cgcatggtcg cgtgatggtg
     2161 ctggacggcg taatccagat caccgagcgc gacgagtttg tctatcagga gatgctgacc
     2221 catgtgccgc tgctggcgca tggggcggcg gcgaatgtgc tgatcatcgg cgcgggtgat
     2281 ggcggcgtgc tgcggcgcgt gctgcagcat cgtggcgtgc agcgcgcggt gatggcggag
     2341 atcgatggcg aggtgatccg tctagccagg gagcacatgc cggatatcgg cggtggggca
     2401 tggaccgatc cgcgcgccga ggtgatcgtc ggcgacggca tcgactatgt gaaacgcgcg
     2461 cccgacgctt cgtgcgatgt gatcatcgtc gattcaacag atccgatcgg tgtcggcgag
     2521 gtgctgttca ccgacgagtt ctatgccaat tgcgcgcgca tcctgacagc acgcgggctg
     2581 gtggtgaacc agtgcggcgt gccgttcatg caggctgacg agctgcgtga cacaagtgcg
     2641 cggcgggcga aattcttccc gcatgtcacg gcgtatgttg cggcggtgcc gacttacgtc
     2701 ggcggattca tgacgttggg gtgggcggcg aaggacccat cgctgacccg cgtcggtgtc
     2761 gatgacgtgc aggcgcgtgc cgacgcggcg ggtattctgg gcaccacgcg gtactggacg
     2821 ccggagatcc atgtgggggc gttcagcctt cctccctata tcggcgaata tttgccgcgc
     2881 gggtgagggg ttgttgtcag tgtacctgta tccacgcgat gatggacggc acgacctggt
     2941 tctcgatgcc gtaataacca tgaggcgata atgcttggca ggggtcgctt cgctgcgacc
     3001 cgccggaaac cgccagcagt tccttaggag aagcctgggt catcagcgcc atcgatgatg
     3061 cggtgtcgct gaatgggctg aagcggcagc cgtcgtcatg attatgcacg accaggactg
     3121 gcatcctgat ctgaccgagc ggcgtgctga gcatgccgcc ctgccagacc gatgatgtga
     3181 ggacggtgcc tgcgatgccc ggtcggccga caaccgcggc accctcggcg gccgagatgg
     3241 atccattgct ggtgccgaca agccagagtg gcgccggcga gcggcttttc aatagcgcga
     3301 caactgcagc gatgtccgtg ccatgctgcg gcgaggcgcg gaacggttgg cccatgccgt
     3361 tcggatggtc ggatggtgta tcgaacaccg cgacagtcat gccggcggcc gaaaactgcg
     3421 gcgcgacccg gaccaggaaa ttgttgcgcg agtgctcgta gacgccagcg gctccgggga
     3481 agagcagaac ggtcgccctc gggttggcag ccgcggttat gtagaccggc tgcgtaactc
     3541 ccggacgcga ttgcacgtca acccgttcct gtgcaatcgc gctgccgatc agcaacagcc
     3601 agatcgcaag aactttcacc accgtgcggc cgcagcgtcg tctgcttcgc gcgcctcgac
     3661 ccaggtggcg gcgccgtcgg ttaattcctc acgcttccag aagggggcct tggttttcag
     3721 ccagtcgatc aggaaggccg tggcatcgag cgcggcctgg cgatgggacg aggcagcgag
     3781 caccagcacg atgttcgcgc cgggggtcag gcggccgacg cggtggatca ccgtgcagcc
     3841 gagcagggac cagcggcgtt cggcttcctc aacgatgcgg gcgatggccc gctcggtcat
     3901 cgccggataa tgctccagtg tcatggcgac gacgtgctcg ccgggattgt tgcgcacggt
     3961 gccgacgaag cagccgatgc cgccgatatc ggtgcgtccg gccgtcagcg cggcgaattc
     4021 gatgccgacg tcgaagtccg cttgctggac actgatgcgg gccaagcggt cagcctcccg
     4081 tcaccggagg aaagaatgca acctcgtccc ccggaccgac acgggtggcc ggctcggcga
     4141 attcctggtt caccgcgcat cggaccgtgc ggcggttggc gaaggcgctc gcttagccgg
     4201 ggcctcgctc cgtcagccag gcgatgagat cggcgaccgt ggcaatgccg gtgggcagtg
     4261 gcaactgctc ctcggacgcg ccgacgcgtt cgcgcagcca ggcgaaatac agaatggtca
     4321 gtggggcttc gacatcggcc atgacgactc aacgtggtgt gggtaccgtc tcgatacggc
     4381 cgtctgaatg tatgatcgtg ctggtgccat tgccattggg aacgatgatc gccgaaccgc
     4441 cgccaggcgt ggtggtggtc tggtagccac tggtgccgcc attggtgacc acggggccgc
     4501 cgggcgtctg gtaaacctgt ccggccgggt ttctcggtgg cggcgtgatg ctcgccggcg
     4561 ggggcgactt cggtagcggc agggcggaca ggccaggttg ggccgatccg ggaggcgtgg
     4621 aactgccgcg gccggcgggc ggcggtggct gatagggtgg tgcacccttc tgctgctgct
     4681 cgaattgtgg tgagccgggc accggctgtt cgggagcggc ctgctctccc tgttgctcca
     4741 gttcctgcag tgtcggttcc gggggcagcg ggccgggcca gatatcgccc ggttcggggg
     4801 tgagcggctt cacgtccacc tgctcgccca cgacccggcg catgttctcg ctgctgccgg
     4861 ccggcatatt ggggttggag cctgagctga acggggggtt ccaccacgtg ttgtaggcgc
     4921 atccggacag cgtcaggcac agaaaggcga aggcgatgcg ccgcatcaag atgggctccc
     4981 accggaacgg ttcgtcggat cgacggccgg acggggccag ccggcgcgga atgtgcgccg
     5041 gtcatgcctg atgctcaagc ggcgatacaa aattcgatga tccaagcggc gatcggggcg
     5101 gggtcgttca acggcagcat tatccggtca gtctccacga ggatatcgct ggccactgca
     5161 acgatgtccg gctgctccgg ccagatcggc ggcttgccga ccgccggccg gtatacttcg
     5221 agcttgggga acgggtgcgt cttgtagcct tcaaccagca ccagatcgac cggctccatc
     5281 cgctccagca gcaccggcag atcgggttcc cggccgacca cctcgtgcag cagagcccag
     5341 cgggtgcccg atgcgacgag gacctcgtgg gcgcccgcct cgcgatggcg gtaggtatcc
     5401 ttcccgggct tgtccatgtc gaagccatga tgcgtgtgct tgacggtgga gatcgtcagg
     5461 ccccgcgcac gcagcagagg aatgaggcgc gtcagcagcg tcgtcttgcc gctgccagac
     5521 cagccgacga taccgagcac cgattgggtc aggggacttg ccgcttcggc atgaccggcg
     5581 agcgcgggac ggatgcgtga tggaggcccg ccatcagata gtcccagccg gtccaaaggg
     5641 tcagcaccgc tgcgacccac agcatgatct cgccaatgcc agtgacaggg atgaaagaaa
     5701 gatgcagcca actggccgat gaatcgcccg ccagcagcgt gccgagggcg cccatctgga
     5761 acccggtctt ccatttggcg agttgggtca ccggcaggcc gatgcgcagc tcggccagat
     5821 attcgcgcag gccgctcacc aggatctcgc gcagcatgat gacgatggct ggatacaggc
     5881 cgagcgccga aagccgacca aggcctacca gcatcatcag cgcggcagcc accaggagct
     5941 tgtcggcgat cgggtcgagc atgcggccga gatcggactg ctgactacgc gagcgcgcaa
     6001 gcctgccgtc gaaaaaatcg gtaatggcgg cggcggaaaa gacgatgcac gctccgatat
     6061 cggccggcgc ctggtgcagc gcgaccagaa caaccagcag cggaatggcg gcgatccgtg
     6121 atagcgtcag caggttgggc aagtcggtga gcatcgttgt cagcctaatc gcatgtctgc
     6181 cgcttcggga ggggggacat ccgcaactgt cacggaactg agatagtcga tcccattcgc
     6241 atcgctagcc cgggggcgaa acgacagcat agccaggggc cccgtatgtc tgaagcactt
     6301 gcggtggccg tcccgacgca gccgaagctc gacaggcgac cacatgtcag tggcgctatc
     6361 attcttatga aactgcctag tatcgacatc gggatcatcc tgaacggcat catcagtggc
     6421 atcgacgccg cgcacgctcc ggcggtcgcc atcggccact tcgtgctgcc gggcaccgcg
     6481 ctgcttgggt acgagttcgt gaacggtttc catgacaccg ccaatgcggt cgcaacggtg
     6541 atctacaccc gcaggctgcc gccacacgtt gcagtcatat ggtcaagtgc gttcaacttt
     6601 ttgggcgtga tcgtgtcgtc gggtgggtct tggcgctcag cttgccgagc ttgttgctgg
     6661 tggagttgat cctgtgatcc tgcagattgg gcagcggcgg cttcgcgatg atctttgcgc
     6721 tgccgaatgc ggcgatcgtc tggaaccttg gcacgtggga ttggcgaacc agctcatggc
     6781 acctgcaggg tcggcgatac acggcgcaaa agaacataac tcgaacgact ggttaatatg
     6841 aggtgcgcga tcgatccggg ccgcgatggg ctccgttacc tggcgcgatg tgcgagactg
     6901 tgatcgcgca tggcgggagt gtcacgcatg acgtcggtcc gtccttcaga tctccaatcg
     6961 ttcctgacct tggccgagac ggcgatcctt cgcggtaccc gcgatgacgg gccggtaccg
     7021 caggcggctg aacgcgtctt ttcggcgctg cgggcctccg tgggacaggt tgggcaggct
     7081 gctcctgcga ggccgcctgt gtgccgtcat ctgacgtcgg cattggagca ggcgcgccgc
     7141 gcacccggcc ctgtcggtgc gctggctgcg gcctttgccg ccgtcgaacc gcggctcgag
     7201 tggaaggcgc gggctggcgc cgaggcgcac ggcgaggcct tcctggccgg gcatgccaac
     7261 gcagcgctga ttggctcgga gggactggaa atccgcggcg atgtctggat cggtgtcagc
     7321 ctcatggcgc cgggtacgcg atatcctgac catcgccatc cgccagagga aatctacgtc
     7381 gtgctgtcgc ccggccaatg gcgacaggcg gacaagccgt ggcatgagcc tggcatcggc
     7441 gggctggtct acaatccacc ggacatcgtg catgcgatgc gctcgatggg ggagccgctg
     7501 ctcgcgttgt ggttcctgcc gacgtgatgc cgagaatcag atcgctgtcc ggccgccatc
     7561 gacgaacaga ttggcgccgt taatgtagct gccggcgtcg gagcaaagga gcatcgcggc
     7621 gccggccaag tcgtcaggcc ggccgaggcg gccggtgggg atgtgcttga cgagggcatc
     7681 gccttccacc gccatctggg cgcggttgcg cgctgtcagg atcgcgccgg gggcgagatt
     7741 gttaactgtg acgccctggc cgccgaactg ccgggccaag cttagcgccc aattgaactg
     7801 tgctgccttg gtgccggcgt agacgaacat cgacgggtgc ggacgctcct gctgcacgct
     7861 gccaatggtg accacacggc cccagcctcg ctccgccatc agtggcacca gtgcctggag
     7921 caattcgagc gtggtgcgga gattgacggc gatctggcgg tc
//
LOCUS       DATGEM010000312         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1057138, whole genome shotgun sequence.
ACCESSION   DATGEM010000312 DATGEM010000000
VERSION     DATGEM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057138"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VKQ27_10015"
     CDS             complement(<1..274)
                     /locus_tag="VKQ27_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLY89309.1"
                     /translation="MIDVLLVIIIFLMLTTTYSRFAELKINLPSAEAEKQLDRPNEID
                     VVISAGGQYLVQRKAVAFRDVANLAQELRQAGANMKDPVVVVNADSS"
     gene            complement(322..1038)
                     /locus_tag="VKQ27_10020"
     CDS             complement(322..1038)
                     /locus_tag="VKQ27_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLY89310.1"
                     /translation="MLIPDPQAKVSAILHPRKFLHHGRDKKLLTIIQAAGWPIYFLLL
                     ASIIAVALIIERFIILRKEKVVPTGLLDSVLDAYQRQGMSGEMLDRLAENSPLGEVLA
                     AGMRNYKSSRYVMKDAIEEAGRAVSHELERFLTTLGTIATVSPLMGLFGTVVGMIEIF
                     GSQSPTGANPQQLAHGISVALYNTGFGLVIAIPAMIFFRHFRARVESFVVEMEQQAAR
                     LVDVVHGQRFDFQPPPRDEA"
     gene            1347..>2462
                     /gene="xseA"
                     /locus_tag="VKQ27_10025"
     CDS             1347..>2462
                     /gene="xseA"
                     /locus_tag="VKQ27_10025"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLY89311.1"
                     /translation="MLGEPELARVSGPLRSEILSVSELNRSIRDLLEHRYPLLWVQGE
                     ISNFTVAKSGHAYFVLKDEDAQVRCVMFRQRAQYLDWSPRDGARVQVQVLLTLYESRG
                     EFQLNVEHMRPAGVGALFEAFLRLREKLEREGLFDVSAKRALPRYPRNIGVVTSLQGA
                     ALRDVLTTLARRNPSIPVVIYPSPVQGEGAADRIAAALRAAGARMECDVLILCRGGGS
                     IEDLWTFNAESIARAIRACPIPVVTGVGHEVDFTIADFAADRRAPTPTAAAELVSPAR
                     EDLLTMIASREERLRVGVHRQLGDRAQRIDLLTRCLIDPEARLRVQSDRLEHLVARLV
                     HGAGRALEERRWMLARLKQRGEACLPDVEQLGQRLVDH"
BASE COUNT          458 a          750 c          759 g          495 t
ORIGIN      
        1 cgctgctgtc cgcgttgacc acgacgaccg ggtccttcat gttcgcgccg gcctggcgca
       61 gctcctgcgc gagattcgcg acgtcccgga aggcgacagc cttgcgctgc accaggtatt
      121 gtccgccggc gctgatgacg acatcgatct cgtttggacg atcgagctgc ttctccgcct
      181 ctgcgctggg caggttgatc ttgagctcgg caaatctgga gtaggtcgtg gtcagcatga
      241 ggaagatgat gatgaccagc aggacgtcga tcatcgggat caggttgatc tcgggatcgt
      301 ccttgcgtgt gcgcgcgaac ttcatgcctc gtcgcggggc gggggctgga aatcaaagcg
      361 ctgcccgtgc acgacgtcga cgagcctggc ggcctgctgc tccatttcca cgacgaagct
      421 ctccaccctg gcgcggaaat gccgaaaaaa gatcatcgca gggatggcga tcacgagccc
      481 gaacccggtg ttgtagagcg cgaccgagat gccgtgtgca agctgctgtg gattcgcgcc
      541 cgtcggggac tgtgatccga agatctcgat cattccgacc accgttccga acaatcccat
      601 gagcgggctg acggtcgcaa ttgtcccgag cgtggtgaga aaccgctcga gctcatggct
      661 cacggcgcgg cccgcctcct cgatcgcgtc cttcatcacg tagcgcgagc tcttgtagtt
      721 gcgcatgccg gccgcgagca cctccccgag cggagaattt tcggccagcc tgtcgagcat
      781 ctctccgctc atgccctgcc gctgatatgc gtcgaggacg ctgtccagaa gaccggtcgg
      841 caccaccttt tccttgcgga ggatgatgaa ccgctcgatg atcagggcga ccgcgatgat
      901 cgatgcaagg agaaggaaat agatcggcca gccggcggct tgaatgatag ttaacaactt
      961 cttatccctt ccgtgatgca aaaatttccg tggatgcaaa atcgcggaca ctttagcctg
     1021 cgggtcggga atcagcaagg cggcggtttg aaacggtttt tctcgaacgt gcgttgatcg
     1081 actgcaatac ttgcgcgggg acttgcggac aattttcccg ctcttttctt atccacagtt
     1141 actgtggata aggttgtgca ttacgcgtcg acagcgaacg taagtaaatc gcaaataagg
     1201 gtttttacca gaatgaccaa atattgaacc gcattcaata tcgcgctgtt tcaataagtt
     1261 acgtaatctc tctcaactct tacctgaagc ccgtcgagta cgcttccgca ggaaagttcc
     1321 cattgtggat aggttagaat gcgccaatgc tcggagaacc agaactggcg cgggtttccg
     1381 gcccacttcg atctgagatc ctgagtgtat cggagctgaa tcgaagcatc cgcgatctcc
     1441 tcgagcatcg ctatcctctc ctgtgggtgc aaggggagat ctcgaacttc accgtggcca
     1501 aatccgggca cgcgtatttc gtgctcaaag acgaggacgc ccaggtccgc tgcgtgatgt
     1561 tccgacagcg agcccagtat cttgactggt cgcctcgcga cggcgccagg gtgcaggtcc
     1621 aggtgctgct gaccttgtac gaatcccgcg gcgaattcca gctcaatgtc gagcatatgc
     1681 gtccggcggg cgtgggcgca ctcttcgaag cgtttctcag gctgagggaa aagctcgagc
     1741 gcgaaggtct gttcgacgtc tcggcaaagc gcgcgcttcc acgctatccg cggaacatcg
     1801 gagtcgtgac gtcacttcag ggagcggcgc ttcgcgacgt cctgacgacg ctagcccgcc
     1861 ggaatccgtc cattcccgtg gtcatctatc cctcgcccgt gcaaggggaa ggcgccgctg
     1921 accgcatcgc cgccgccctg cgggccgcgg gggcgagaat ggaatgcgac gtgttgatcc
     1981 tttgccgggg cggcggaagc atcgaggatc tgtggacatt caacgcggaa tcgatcgccc
     2041 gagccatccg cgcctgtccc attccagtcg tcactggcgt cggccacgag gtcgatttca
     2101 ccatcgcgga cttcgccgcg gatcggcgcg cccccacgcc gacggcggcc gccgagctgg
     2161 tcagcccggc ccgggaggat cttctgacga tgatcgcatc ccgggaggaa cggcttcgcg
     2221 tcggcgtgca tcggcagctc ggagaccggg cgcagcgcat cgacctgctg acccgctgcc
     2281 tgatcgatcc ggaggcgcgc cttcgggttc aatccgaccg gctcgagcac ctcgtcgcgc
     2341 gtctcgtcca cggcgccggg cgggctctgg aggaacgccg ctggatgctc gcgcgcctga
     2401 agcaacgcgg cgaggcgtgc ctgccggacg tcgagcagtt gggccagcgc cttgtcgatc
     2461 ac
//
LOCUS       DATGEM010000313         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_721777, whole genome shotgun sequence.
ACCESSION   DATGEM010000313 DATGEM010000000
VERSION     DATGEM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721777"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VKQ27_10030"
     CDS             <1..572
                     /locus_tag="VKQ27_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HLY89312.1"
                     /translation="STIGSPLCADCSRVWQIDALRAREPGGAAWRCALAMAYYTKVLQ
                     PDEQVRVIGHLHWSIYLRALFVLVVALVVAVGSAWLADPDWQRIALWAAAAIAVIGLL
                     MLFGAWVRRRATEIVVTDRRVIFKRGLVSRHTVEMNVGKIETVDVEQGLGGRLWGYGT
                     VMIHGTGSGFEPLVGIGAPIQIRNAIVAG"
     gene            621..932
                     /locus_tag="VKQ27_10035"
     CDS             621..932
                     /locus_tag="VKQ27_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89313.1"
                     /translation="MRKFLLLLPEWTPDWVLLPVCDPEQRRLMLWSYGLMMRVRLMSV
                     VVALVEPVATRGLVTVLRRHADALSAEVAAWRRAVWESTPDRSHKPPVLPVPACKNPW
                     I"
     gene            1275..2063
                     /gene="rpsB"
                     /locus_tag="VKQ27_10040"
     CDS             1275..2063
                     /gene="rpsB"
                     /locus_tag="VKQ27_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLY89314.1"
                     /translation="MAMPDYSMRQLLEAGVHFGHHTRRWNPAMSPYLYGVRNQVHIID
                     LQQTVPLLDRALRAVRDVTAAGGRVLFVGTKRAAAEYVADAAKRCGQYYVNHRWLGGT
                     LTNWKTITGSIKRLRQIEEQLGGNTEGLTKKEVLDITRDKEKLDRALGGIKEMGGLPD
                     ILFIIDTNKEKLAVEEANKLRIPVVAVLDSNSDPRGVTYPVPGNDDAIRAITLYCDLI
                     AGAVLDGISAEMAASGQDIGAVEELPPEVMPEVEVPAEAANPSL"
     gene            2082..2993
                     /gene="tsf"
                     /locus_tag="VKQ27_10045"
     CDS             2082..2993
                     /gene="tsf"
                     /locus_tag="VKQ27_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003623141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLY89315.1"
                     /translation="MPEITAALVKDLREKTGAGMMDCKKALTETGGEIEPAVDWLRQK
                     GLAAAAKKSGRVAAEGLIGVASTSGRAAMVEVNAETDFVARNEQFQAFVEAVAKIALR
                     VGEDLDAIKAAQYPDSAHTVAEQLTELIAKIGENMNLRRARVLNAPNGVVATYVHSAL
                     RPGLGKIGVLVAIEGGGEMAALETLGRQVGMHVAAARPEALDVAAVDPAALEREKAVL
                     IAQAQESGRPPNIIEKMVEGRIRKYYEEVVLLEQVWVHDGESRVKAVVSKAGVKLGGF
                     ARFQLGEGIDKPEGPDFASEVAATAGV"
     gene            3126..4016
                     /locus_tag="VKQ27_10050"
     CDS             3126..4016
                     /locus_tag="VKQ27_10050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89316.1"
                     /translation="MSVQLGPLRPDFVAEVSGLDLAQPMDTATVDALWSAIDQYAVLI
                     FRDQRLTDMQLRDFAARFGPLEIGRAAARPGRRRIEIPQIGDISNLDINNNVRALDDR
                     RRLDFLGNRLWHTDASYMPVPVVLGMLHAVALPPPSPFGNGETEFADMRAAYDALPDN
                     TKQAIDGLAVEHDIFWSRGLIGVTEFPPGEREQYPPSKQRLVRTHPGSQRKTLYLSAH
                     ASHVVGWPVADGRLLLWDLSAHATQQQFVYSHSWRVGDLVIWDNRVTMHRGRPHDEKQ
                     PRDLRRATTLDTANTLDQAA"
     gene            complement(4013..>4964)
                     /locus_tag="VKQ27_10055"
     CDS             complement(4013..>4964)
                     /locus_tag="VKQ27_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HLY89317.1"
                     /translation="VSNLPALYDSRPNDPSARKFGGYIMWTTFAANCITSSLFMTACA
                     PNFLAIEFIRKIVHVDITYVQWMKGSLPFALPLLLALPLLVYWLYPPQVKRSPEVSVW
                     AGGELRKMGAISKNEIILSVLVIGAILLWVLGTSIIDPTLTALAGIGLMLVCGVVTWD
                     DMAKNHSAWTTLTLLATLVTLADGLSRAGFIKWFADFVAAHVGGYSPTLILVMMVAVY
                     FLSHYMFASLTAHTTAMMPMMLAAGMGIQGLPAPTLAMALALTTGIMGVITPYATGPG
                     LAYYNSGYLPPADFWRLGTIFGLIFLAALLLIGVPLLAMG"
BASE COUNT          878 a         1555 c         1689 g          842 t
ORIGIN      
        1 cgagcacgat cggttctccc ctttgcgcgg actgctcacg ggtatggcaa atcgacgccc
       61 tgcgcgccag ggaacccggg ggcgcggcgt ggcgttgtgc gttggcgatg gcgtactaca
      121 cgaaggtgct gcaaccggat gagcaggtga gggtgatcgg ccacctgcac tggtcgatct
      181 atctgcgtgc cctgttcgtg ctggtggtcg ctttggttgt ggcggtgggc tcggcgtggc
      241 tggctgaccc ggactggcag cgcatcgcgc tgtgggccgc ggcggcaatt gccgtgatcg
      301 ggctgctgat gctgttcggc gcctgggtgc ggcgccgggc gaccgaaatc gtggtgaccg
      361 accgccgggt gatcttcaag cgtggcctgg tgtcgcgtca caccgtggag atgaacgtcg
      421 gaaagatcga gacggtggat gtggagcaag ggctgggtgg ccggctgtgg ggctacggca
      481 ccgtgatgat ccacggcacc ggctcgggtt ttgagccgct ggtcggcatc ggcgcgccga
      541 tccagatccg caacgccatc gtcgccggat agggcgcggc ggttaaccag ccgttagcgc
      601 tgccgcgtcc atgctgcatc atgcgcaagt tcctgctgct cttgcccgag tggaccccgg
      661 actgggtgct gctgcctgtc tgcgatccgg agcagcgtcg cctcatgctg tggagctatg
      721 ggctgatgat gcgggtgcgg ctcatgtcgg tggttgtggc gctggtcgag ccggtggcca
      781 cgcgcgggct ggtcacagtg cttcgccgcc acgcggatgc gctgtcggct gaggtggccg
      841 catggcggcg cgcggtgtgg gagagcactc ccgaccgttc gcacaagccg ccggtactgc
      901 cagtgccggc gtgcaagaac ccgtggatat gatcggccgt gccatgagcg tgggttggcc
      961 cacgtaatca taaatccagc catggatggc tcgacgatga cagagctaag ccgcgccttc
     1021 gcatccgcgg cactccagct catatgggcg acctttggtc acgatggacc ataatagtca
     1081 ctgcgacccg ccgcggcagg ttcgcatcca ccttgcgttt cgcctgcttc tcggccatat
     1141 cccgcgcgcc ggcccatgcc ggtaattcac acgcggggtg cctttcccgg ccaggcttag
     1201 tcctcgggcg ggtccggtgt cgcaagacag cccccgcgga ggcacaaccg gacgaagcga
     1261 aaggaaccgc ccctatggcg atgcccgatt attccatgcg tcagctgctg gaagcaggcg
     1321 tgcatttcgg ccaccacacg cgccggtgga acccggcaat gtcgccgtac ctgtatggtg
     1381 tgcgcaacca ggttcacatc atcgatctgc aacagacggt gccgctgctc gaccgtgcac
     1441 tgcgcgccgt tcgtgacgtg accgctgccg gcgggcgcgt gctgttcgtc ggcaccaagc
     1501 gggctgcggc tgaatacgtc gccgacgcgg ccaagcgctg cgggcagtat tacgtcaacc
     1561 accgctggct cggcggcacg ctgaccaact ggaagacgat cacaggctcg atcaagcgcc
     1621 tgcgccagat cgaggagcag cttggcggca acacggaagg cctcaccaag aaggaggtgc
     1681 tcgacattac gcgcgacaag gagaagctcg atcgcgcgct tggcggcatc aaggagatgg
     1741 gcggcctgcc ggatatcctg ttcatcatcg acaccaacaa agagaagctg gcggtcgagg
     1801 aggcgaacaa gctccgcatt ccggtggtcg cggtgctgga cagcaattcc gatccgcgcg
     1861 gcgtgaccta cccagtgccg ggcaacgacg acgcgatccg cgccattaca ctgtactgcg
     1921 acctgatcgc cggcgcggtg ctcgatggga tcagcgccga gatggctgcc tcgggccagg
     1981 atatcggcgc ggtggaggaa ctgccgccgg aagtcatgcc ggaggtggaa gttccggctg
     2041 aagccgccaa tccgtcacta tgatcatcgg agaccgaacc gatgcctgag atcactgctg
     2101 ccctggtgaa ggacctgcgc gagaagaccg gcgcgggcat gatggactgc aagaaggcgc
     2161 tgaccgagac cgggggagag atcgaacccg cggtcgactg gctgcgtcag aagggccttg
     2221 ccgccgcggc caagaagtcg ggccgcgtcg ccgcggaggg gctgattggc gtcgcgtcga
     2281 cgtcgggccg ggcggccatg gtcgaggtga acgccgagac cgattttgtc gcgcggaacg
     2341 aacagtttca ggccttcgta gaggctgtgg cgaagatcgc gctgagggtg ggcgaggacc
     2401 tcgatgcgat caaggccgcg cagtacccgg acagcgccca caccgtcgct gagcaactga
     2461 ccgagctgat cgccaagatc ggcgagaaca tgaacctgcg gcgtgcacgt gtgctgaacg
     2521 caccgaacgg tgtggtcgcg acctatgtgc attcggcgct gcgccctggg cttggcaaga
     2581 tcggcgtgct ggtcgccatt gaaggcggcg gtgagatggc ggcgctggag acgctcggcc
     2641 gccaggttgg catgcacgtc gcggcggcac gacctgaggc gctggatgtc gctgccgtcg
     2701 atcccgccgc actggaacgc gagaaagcgg tgctgatcgc gcaggcccag gagtcgggcc
     2761 gcccgccgaa catcatcgag aagatggtcg agggccgcat ccgcaagtac tacgaggaag
     2821 tggtgctgct cgagcaggtc tgggtgcacg acggcgagag ccgcgtgaag gccgtcgtgt
     2881 cgaaggcggg cgtgaagctg ggcgggttcg ctcggttcca gttaggcgag gggatcgaca
     2941 agcctgaggg tccggacttc gccagcgagg tcgccgcgac ggctggggtc taagcggact
     3001 gcctgcatcg gccgcggagc gggatgccgc gccgccggtg ccgcttccgg gacatcgcgt
     3061 aggcttggtt ccttgcgccg gccggcgctc gggtgtagcc gctgacggca aggagggagt
     3121 gtgccatgtc cgttcagctc gggccgctgc gtcccgactt tgtcgccgag gtgtcgggcc
     3181 ttgatctggc gcagcccatg gacaccgcca ccgtcgacgc cctgtggtcc gcgattgacc
     3241 agtacgcggt tctgatcttt cgcgaccagc gcctcaccga tatgcagttg cgcgacttcg
     3301 ccgcgcgctt cggcccgctg gagatcggcc gtgccgccgc tcgccccggc cggcggcgca
     3361 tcgaaatccc gcagatcggc gacatatcca acctcgacat caacaacaac gtgcgcgccc
     3421 tggacgacag gcgcaggctt gacttccttg gcaaccggct ttggcacact gatgcttcct
     3481 acatgccggt gccggtcgtg cttggcatgc tgcacgccgt cgcgctgccg ccgccgtcgc
     3541 cgttcggcaa cggcgagacg gagttcgccg acatgcgtgc cgcttatgac gcgcttcccg
     3601 acaatacgaa gcaggccatc gatggtctcg cggtggagca cgacatcttc tggtcgcgtg
     3661 gcctgatcgg tgtcaccgag ttcccgcccg gcgagcgcga gcaatacccg ccctcgaagc
     3721 aacggcttgt gcgcacccat ccaggatcgc agcggaagac gctctatctg tcagcccacg
     3781 catcgcatgt ggtcgggtgg ccggtggcgg acggccgact gctgctatgg gatctcagcg
     3841 cgcatgcgac gcagcagcag ttcgtctaca gccattcatg gcgcgtcggt gatctggtga
     3901 tctgggataa tcgcgtcacg atgcatcgcg gccggccgca tgacgaaaag cagccgcgcg
     3961 acctgcgccg cgcgactaca ttggacacgg ccaacacgct ggatcaggca gcctagccca
     4021 tcgccagcaa cggcacgccg atgagcagca gcgctgccag gaagatcaac ccgaagatgg
     4081 tacccagccg ccagaagtct gccggcggca gatagccgct gttgtagtag gcaagccccg
     4141 gtccggtcgc atatggcgtg atcacgccca tgatgccggt ggtcagtgcc agggccatcg
     4201 caagcgtcgg cgcgggcaat ccctggatgc ccatgcccgc tgccagcatc atcggcatca
     4261 tcgccgtggt gtgcgccgtc aggcttgcga acatgtaatg cgagaggaaa tataccgcga
     4321 ccatcatcac caggatgagc gtcggcgagt agccgccaac gtgtgccgcg acgaaatcgg
     4381 cgaaccattt gatgaagccc gcgcgggaca gaccgtcggc cagcgtcacc agggtcgcga
     4441 gcagcgtaag tgtcgtccac gccgagtggt tcttcgccat gtcgtcccag gtgaccacgc
     4501 cacacacaag catcagcccg atgccggcca gagccgtcag cgtcggatcg atgatgcttg
     4561 tgccaagcac ccacagcagg atcgcgccga tcacgaggac cgaaaggatg atctcgttct
     4621 tcgagatcgc gcccattttc cgcagttcgc cgcctgccca tacagacacc tccgggctgc
     4681 gcttcacctg cggcggatac agccaataga ccagcagcgg cagcgccagc agcaatggca
     4741 gtgcgaacgg caacgagccc ttcatccact gcacatacgt gatgtccaca tgcacgatct
     4801 tgcggatgaa ctcgatcgcc aggaaattcg gcgcgcacgc tgtcatgaac agtgaactcg
     4861 tgatgcaatt agcggcaaac gtcgtccaca tgatgtagcc gccgaatttc cgcgccgacg
     4921 gatcgttcgg tcgcgagtca tagagcgcgg gcaggttgct cacg
//
LOCUS       DATGEM010000314         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1510416, whole genome shotgun sequence.
ACCESSION   DATGEM010000314 DATGEM010000000
VERSION     DATGEM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510416"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VKQ27_10060"
     CDS             <1..378
                     /locus_tag="VKQ27_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HLY89318.1"
                     /translation="IAQAADAPSELSLMHLYPIDGAVHRVPKDATAWTTRDATWSMVI
                     AGIDAHPEQADRLKTWGRGYWNAIHPFNLAGAYVNFMMDDEVQGRIQASYGENYQRLA
                     SVKATYDPQNLFRVNQNILPAAA"
     gene            complement(1063..2097)
                     /locus_tag="VKQ27_10065"
     CDS             complement(1063..2097)
                     /locus_tag="VKQ27_10065"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLY89319.1"
                     /translation="MMETHFTRLVGCELPIQMAGMGGVAIPPLVVAVCRAGGLGMLAG
                     DGSSPETMQRLLDDVRSGTDRPFGVSFLMPYLREPACVDAAAAAARVVEFFYGAPDAQ
                     FVERVHAAGALACWQVGSADEAQTADQVGCDLIVAQGVEAGGHIRGQIGLLSLLDQVL
                     RRVEVPVIAAGGIGSGRAMATALAGGAAAVRVGTRFVAALESPAHPLYVEQLVEARAE
                     DTEVTEGFDLAWPDAPHRVLRSAIEAARGAPDEVIGANHDYFDPRRSSPVLRWQVLTP
                     TVLTTGNIAAMPLWAGESVNFVASRQSAAEIVRDLVNEAAPLLAHGFPCADSKPDSRI
                     GAPFSPSQPH"
     gene            complement(2255..2677)
                     /locus_tag="VKQ27_10070"
     CDS             complement(2255..2677)
                     /locus_tag="VKQ27_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HLY89320.1"
                     /translation="MGGRAVPGQDDRASGQRTRRRPGRRRSWQATAYRRSVQVAQNAV
                     EAPGAMQAICHLSFGDYSGADYAAQLFMDALIHGWDIAKGTGQNTRLDPELVAACHPI
                     AEELTRQFRQAGVFGEDLPVSADADPQTRLLALLGRSA"
BASE COUNT          518 a          937 c          919 g          470 t
ORIGIN      
        1 atcgctcaag ccgccgatgc gccgagcgag ctgtcgctga tgcacctgta cccgatcgat
       61 ggcgccgtcc accgcgtgcc gaaagacgcc accgcctgga caacgcgcga tgccacctgg
      121 tcgatggtca tcgccgggat cgacgcacac cccgagcagg cggatcggtt gaagacgtgg
      181 ggccgcggct actggaacgc aattcacccg ttcaatctgg caggcgccta cgtcaacttc
      241 atgatggacg acgaggttca agggcgaatt caggccagct atggcgagaa ctaccaacgt
      301 cttgcctcgg tgaaggcgac gtacgatccg cagaacctgt ttcgggtcaa ccagaacatc
      361 ttgcccgccg cggcctgaga gaacgaggtc atcggggcac gaatagatgg acgaacgacg
      421 cggggcttgg gaggtggtgg gcgccgtgac tgcggtctac gcggccggcg agcgacagga
      481 cttcgcagca cgccgcgcac tccgtggagc cgccgcgtcg ttgggtacat acgacacaga
      541 gctcgcgtgg tgaatcggaa ggttattgat tgccgagatt tcacgaacgc gatcgactgc
      601 actctgggga gctcagcggc ggagtcatcg tcgcatagca ggcccggcgt cacgacggcg
      661 tgcctcgctc gcacaactct gcaatccatc cgctgctagt gtcgcctcaa gtccgtcaac
      721 attcggcagt cggcataagg ggcaatcagg tagacgtact gcaagacgcg gatgagtgtg
      781 cggctcgcac ccgcccactg catcatcgct acggcacaca cgacagtcag cagagaaccg
      841 atctcctgca accagccggc aagcacatag ttgcggcatg ttgagcgccg agctccagga
      901 tgtcctggat gccggcatgg caccagccag cgcggtcagt ccgcgaaaca atctatcaga
      961 ttgtttcgcc cccggggtgc attggggcag tgcctttcgt gctgcgcagc gtcaggtgag
     1021 ggctgcgatt tgcgtccagg cggcgggaac gacgagcgca catcaatgtg gttgcgatgg
     1081 gctgaatggc gcgccgatcc gggaatccgg cttcgaatcg gcgcacggaa atccatgcgc
     1141 gagcagaggc gctgcttcgt tcaccaggtc gcgcacgatt tccgccgccg actggcggct
     1201 ggcaacgaaa ttcaccgatt cgccggccca cagtggcatc gcggcgatgt tgccagtggt
     1261 gagcaccgtc ggcgtgagca cttgccagcg cagtactgga ctgctgcggc gcgggtcgaa
     1321 gtagtcatga ttcgcgccga tcacttcgtc gggggcgcca cgagccgctt caatggcgga
     1381 ccgcaatacg cggtgaggcg catcgggcca cgcaaggtcg aacccttcag tcacctcggt
     1441 gtcttccgcg cgagcctcca ccaactgttc gacatacagc ggatgcgccg gcgactccag
     1501 cgctgccacg aagcgcgtgc cgacgcgtac cgcggccgcg ccgcctgcca atgcagtagc
     1561 catggcgcga ccgctgccga tgccaccggc ggcgatgacc ggtacttcca cgcggcgcag
     1621 cacctggtcg agcagactca gcagtccgat ctggccgcgg atgtgaccgc cggcctcgac
     1681 accctgcgcc acgatcagat cgcagccaac ctggtcggcg gtctgcgcct cgtcggcgga
     1741 accaacctgc cagcaagcta gtgcgccagc cgcgtgcacg cgctccacaa attgtgcgtc
     1801 aggagcgccg tagaagaatt ccaccactcg cgccgcggcg gcggcggcat cgacgcacgc
     1861 gggctcgcgg aggtacggca tcaggaaact gacgccaaag ggccggtcgg tgccagagcg
     1921 cacgtcgtcc aacaatcgct gcattgtctc tggtgaggag ccatctcctg ccagcatgcc
     1981 gagcccaccg gcgcggcaca ccgccaccac cagcggagga atcgcgactc cgcccatgcc
     2041 cgccatctgg atcggtagct cgcagccgac cagccgcgtg aaatgcgtct ccatcatggc
     2101 cgtgcctcct gtagcggcgc gtgcgcgaag cgatcgacgt gcggttgaag gatgtctgct
     2161 ggagcgtcgt tgaccgtagc ctcgcgatcg acgaataccg gctccggctg gacggctgcg
     2221 acgcggagat gctggccaga taccgcaggt ctcgctacgc actgcgcccg agcagcgcca
     2281 gcagccgtgt ttgaggatcg gcgtccgcgc tcacgggcag gtcctcgcca aacacgccgg
     2341 cctggcggaa ctggcgcgtc agctcctcgg cgatcgggtg acacgccgcc accagctcgg
     2401 ggtcgagccg cgtgttctgg ccagtaccct tggcaatgtc ccagccgtgg atgagcgcat
     2461 ccatgaatag ctgggcggcg taatcggcgc ccgagtaatc gccgaacgag aggtggcaaa
     2521 tcgcctgcat cgcaccaggc gcctcgacgg cgttctgggc gacctgtact gagcggcgat
     2581 aggcggtcgc ctgccaggat cgtcgcctgc caggtcgccg tcgagtgcgt tgcccacttg
     2641 ctcgatcgtc ttgccctgga acagctcggc cgcccacaga ttctcgctaa cgatgtggct
     2701 ggcgacctgc cgtgcgtccc actccgtgca tggcgtcgag gcgtgccagc ggtcagcggg
     2761 aaccccctcg atgatccgct gcgtcgccgc catcgctcgt acgtagaggt caccgttagt
     2821 catcttcgtc gtcgtcacta gtgt
//
LOCUS       DATGEM010000315         5847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_352619, whole genome shotgun sequence.
ACCESSION   DATGEM010000315 DATGEM010000000
VERSION     DATGEM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352619"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..421)
                     /locus_tag="VKQ27_10075"
     CDS             complement(230..421)
                     /locus_tag="VKQ27_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLY89321.1"
                     /translation="MEELVIEDAEACQLAAELAELTGHSLRLAVVTALREERDRALSR
                     RRRHERIMAITREIALSAA"
     gene            complement(441..1301)
                     /locus_tag="VKQ27_10080"
     CDS             complement(441..1301)
                     /locus_tag="VKQ27_10080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HLY89322.1"
                     /translation="MQGTKPSFQALILRLHDYWARQGCVILQPYDVEMGAGTFHPATT
                     LRSLGAKPWRAAYVQPSRRPTDGRYGENPNRLQHYYQYQVIMKPSPEDAQDLLLGSYR
                     ELGLDPLVHDFRFVEDDWEGPTLGAWGLGWEVWCDGMEVGQFTYFQQMGGIPVVAPSF
                     EMTYGLERLAMYVQDVENVYDLDFNGAGVSYGDVFLRAEREYSAFNFEHADTAMLTRH
                     FADAETECGALLEQRLPLPAYDQCIKASHLFNLLDARGVISVTERASYIGRVRALAKG
                     CCEAWVATDN"
     gene            1379..1807
                     /locus_tag="VKQ27_10085"
     CDS             1379..1807
                     /locus_tag="VKQ27_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLY89323.1"
                     /translation="MSLDGQTDDTIRAILSRTRRIAVVGASDKPWRPSNGVFRFLLAQ
                     GYDATPVNPTIAGTAIHGRPVVAFLAEAAPLDMVDIFRASANAGAAVDEAIRLGARSV
                     WMQLGVIDRAAAERARAAGLAVVMDRCPAIELPRLGIARA"
     gene            1861..2751
                     /locus_tag="VKQ27_10090"
     CDS             1861..2751
                     /locus_tag="VKQ27_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HLY89324.1"
                     /translation="MTNEERDIITQFIARVGGAQAAQPSFGGSVPATTTPALPPVDPE
                     ADRFIADLFTRYPEARYRITQLAFVQEHALTEAQNRLQRMDWELQQARQQAQQAPQPQ
                     QAGGSGFFGGLFGGGSRPSAPPPQQGGPAWNQGAQQPQYQQYQQAPPPPPQYAPGYQP
                     GMFQRQGSGFLGSALTTAAGVAGGLVAGNALMNLFSGGHGGGFGGGFGGGGNTTIIEE
                     NNSPWGGAAGSVDQGTWDTSGGGGSGANPGNVDNGTWDTSGGGSDQSSWTDNSSSDSG
                     WDNSGGGDASWDTSSPDDST"
     gene            complement(2743..3087)
                     /locus_tag="VKQ27_10095"
     CDS             complement(2743..3087)
                     /locus_tag="VKQ27_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLY89325.1"
                     /translation="MSPQRHSPAAVFAALGDPTRLALVGKLSVGTPLSIARLTDGSDL
                     TRQAVTKHLRVLESSGVVGKRPQGRESLYQLRPAAIQDMRAYLDEISRQWDDALARLK
                     TFVEAEAVPPSG"
     gene            complement(3068..3520)
                     /locus_tag="VKQ27_10100"
     CDS             complement(3068..3520)
                     /locus_tag="VKQ27_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLY89326.1"
                     /translation="MSDRIEKQIDIKAPVARVWRAIADYREFGTWFRVAMEGPFVAGQ
                     EARGRITYPGKEQLVMTVTVQRIEPERLLSFTWHPYAIDPNTDYSAEPATLVEFILEP
                     AAEGTRLTVVESGFDALPPHRRDLAFRMNEGGWAIQVRNIAQHVAATP"
     gene            complement(3631..4368)
                     /locus_tag="VKQ27_10105"
     CDS             complement(3631..4368)
                     /locus_tag="VKQ27_10105"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HLY89327.1"
                     /translation="MRPIVVIPTRLGSTRLPGKALTDINGSPMIAHVWRRGIEADIGP
                     VVVACGDQPIADTIQDLGGRAVLTDPALPTGSDRAYAALGMVDPDGAYDVVVVLQGDL
                     PTIDPVTVRATLRPLRRDADVSIATLATEINDPLELQAPQVVKIALAVKPGDTVGRAI
                     YFSRAAIPAGAGRHYHHVGMYTYQREALARFVRLPRGILEQRESLEQLRALEFGMRID
                     AAVIETEPLGVDTLADLERARELLAQA"
     gene            4484..5338
                     /locus_tag="VKQ27_10110"
     CDS             4484..5338
                     /locus_tag="VKQ27_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY89328.1"
                     /translation="MTTISNAMPPTRGMIGLTVRAAERFGVQALIACGALLIVVPLLL
                     TLYLSVFDEKLILFPPRGYTLTWYPAILPNFGDAIVTSLELGAAAVVGSLLLGVPAGI
                     GLARHRFRGRGAISTLLLAPLTVPGIALGLSVYVGLVAIDERFGSAVTGSMAGLVLAH
                     IMLTTPWVVRLCLASLTNHEHAAEEAAASLGAGPLRVIWRVTLPAMRSGIIAAALFAF
                     IFSFENLELALFLTSPGVTTLPVAVLQYLEYHIDPLVSAVAVAQIVAVAALLLVLDRF
                     VRLGEVVQ"
     gene            5335..>5847
                     /locus_tag="VKQ27_10115"
     CDS             5335..>5847
                     /locus_tag="VKQ27_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89329.1"
                     /translation="MSDAHALALEGLSKQFGTQTAVDEVDLAVRPGELIVLLGPSGCG
                     KTTTLRMIAGFVPPSGGDIRVDGSSIVALPAHRREMGIVFQSYALFPHLSVARNIAFG
                     LEMRRMAAAAIASRVAEMLRLVKLEAFADRLPRQLSGGQQQRVALARALAINPRVLLL
                     DEPLSNLDASL"
BASE COUNT          948 a         1965 c         1921 g         1013 t
ORIGIN      
        1 ggtggggaag gccgattcct gggtagaact agtgatccat ccgaccagtg tcgagcgccg
       61 gtgcttccga tgacgtgaca acatcaccca acaaccagtc ctggcacctg cacgcgatag
      121 ttttgtttca aacacggaga aggccgagga ccacggaggg cacaggagtc ggagcggccc
      181 cgactcctgt gccctccgtg gtcctcagcc ttctccgtgt tgacgaatcc tatgccgcgc
      241 tcagcgcgat ctcgcgggtg atcgccatga tgcgctcgtg ccggcgccga cgggacagtg
      301 cccgatcgcg ttcctcgcgc agtgccgtca cgaccgccaa acggagactg tggcccgtca
      361 gttccgccaa ctccgctgca agttggcagg cctcggcatc ctcgatcacc agttcttcca
      421 tgtcgtttcc ttccgcatca tcagttatcc gtagcaaccc aggcctcgca gcagcccttc
      481 gcgagggcgc gcacgcggcc gatatagctc gcgcgttcgg tgacgctgat caccccgcgc
      541 gcatccagca gattgaacag gtggctcgcc ttgatgcact ggtcgtaggc cggcagcggc
      601 aaccgctgtt ccagcagcgc gccgcactcc gtctcggcat ctgcgaagtg ccgcgtcaac
      661 atcgctgtat ctgcatgttc gaagttgaac gcgctgtatt cgcgctcggc gcggaggaag
      721 acatcgccgt agctcacgcc agcaccgttg aagtccaggt cgtagacatt ctcgacgtcc
      781 tgcacataca tcgcgagccg ctccagcccg taggtcatct cgaacgacgg cgcgacgacc
      841 ggaatgccgc ccatttgctg gaagtaggtg aactgcccca cctccatgcc gtcgcaccac
      901 acttcccagc caagacccca ggcgccgagc gtcggcccct cccagtcatc ctcgacgaag
      961 cggaagtcgt gcaccagcgg gtccagcccg agctcgcgat agctccccag caggaggtcc
     1021 tgagcgtctt ctggcgacgg cttcatgatg acctgatact ggtaatagtg ctgcagacgg
     1081 ttcgggttct cgccgtagcg gccgtcggtg ggacgccgcg atggctgcac atacgccgcc
     1141 cgccacggct tcgcccccag cgaccgcaac gtggttgccg ggtggaatgt tccggcgccc
     1201 atctcaacat cgtagggttg cagaatcaca cagccttgcc gcgcccagta gtcatggagc
     1261 cgcaggatca gtgcctgaaa actgggcttc gtgccttgca ttcgaccggg cgatgttgat
     1321 tgcggcgcca catagagtac ctgtggacaa ccggcaacac ccttgtaagg cggaattcat
     1381 gtcactcgat ggccagaccg acgacacgat ccgggccatc ctgagcagga cccgccgcat
     1441 cgccgtcgtc ggcgcctcgg acaagccttg gcggccgtcg aacggggtgt tccgtttcct
     1501 gctggcccag ggctacgacg cgacgccagt caatccgacg atagccggca ccgccatcca
     1561 cggccgcccc gtggtcgcct tcctggccga ggcagcgccg ctcgacatgg tggacatctt
     1621 ccgcgcctcc gccaatgcgg gggcggcggt ggacgaggcg atccggctcg gcgcccgcag
     1681 tgtctggatg cagcttggtg tgatcgaccg cgcagcggca gagcgggcac gcgccgcggg
     1741 gctggccgtg gtaatggacc ggtgtccggc gatcgagctg cctcgtctcg gtatcgcacg
     1801 tgcttgaagg gaagcggcgc ccgcgccaca tcgcagccac ggaacccagg agggtgccca
     1861 atgaccaatg aagagcgcga catcatcacc cagttcattg cccgcgtcgg tggcgcgcag
     1921 gcggcacagc cgtcgttcgg cggctcggtg cccgcgacca cgacaccggc actgccgccg
     1981 gtcgatcccg aggcggatcg cttcatcgcg gatcttttca cgcgctaccc cgaggcgcgt
     2041 taccgcatca cccagcttgc cttcgtgcag gagcacgcgc tgaccgaggc gcagaaccgc
     2101 ctccagcgga tggactggga gctgcagcag gcccgccagc aggcgcagca ggccccgcaa
     2161 ccgcagcagg cggggggtag cggtttcttt ggcgggctgt tcggcggtgg ctcgcgcccg
     2221 tcggcgcctc cgccgcaaca gggcgggccg gcatggaacc agggagcgca gcagccgcag
     2281 tatcagcagt accaacaggc cccgccgccg ccgccgcagt acgccccggg ctatcagcct
     2341 ggcatgttcc agcggcaggg gtccggcttc ctcggttcgg cgctgaccac cgcggccggc
     2401 gtggcaggcg gtctggtcgc cggtaatgcg ctgatgaacc tgttctccgg tggacatggc
     2461 ggcgggttcg ggggtggctt cggcggcgga ggcaacacca cgatcatcga agagaacaac
     2521 agcccctggg gcggcgccgc cggcagtgtc gatcagggca catgggacac atcgggcggc
     2581 ggcggttctg gagccaatcc cggcaatgtt gacaacggca cgtgggatac ctctggcggc
     2641 ggcagcgacc agtcgtcctg gaccgacaac agcagcagcg attcaggctg ggacaacagc
     2701 ggcggtggtg atgccagctg ggacaccagc agcccggacg actcaacctg aaggcggcac
     2761 cgcttcggct tcgacgaacg tcttgaggcg ggccagcgcg tcgtcccact gacgggaaat
     2821 ctcgtcgaga tacgcgcgca tgtcctggat cgctgccggc cggagctggt acaggctttc
     2881 gcggccttgg ggccgtttgc cgaccacgcc gctgctttcc agcacgcgca ggtgcttggt
     2941 gacggcctga cgcgtgaggt ccgagccatc ggtcagccgc gcgatggaca gcggagtgcc
     3001 gacgctcagc ttgccgacca gtgcgagccg cgtcggatca ccgagcgcgg cgaacacggc
     3061 cgccgggcta tggcgttgcg gcgacatgtt gggcgatgtt ccgcacctgg atcgcccagc
     3121 cgccctcgtt catgcggaac gccaggtcgc ggcgatgcgg cggcagggcg tcgaagcctg
     3181 attcgacgac tgtaagtcgt gtgccttccg cggcgggctc cagaatgaac tccaccagcg
     3241 tggcgggttc ggccgaataa tcggtgttcg ggtcaatcgc atagggatgc caggtgaaag
     3301 aaagcaaccg ctccggttcg atgcgctgca cggtgactgt catcaccagt tgctccttgc
     3361 ctggataggt gatccggccg cgcgcctcct ggccggccac gaacggccct tccattgcga
     3421 cgcggaacca cgtgccaaat tcgcggtaat cggcgatggc gcgccatacg cgagcgaccg
     3481 gcgccttgat gtcgatctgc ttttcgatgc ggtcggacat gcgcaacctc ctggttgcct
     3541 tttggttaca gcgttccgcg aagatatgca accctgaagt tgcatatctg gccctttctg
     3601 gttggcaaca gcagttttcc ggacgcgcac ctaggcttgc gccaataatt cgcgcgcccg
     3661 ctccaggtca gccagggtat cgacaccgag cggttcggtc tcgatcactg ccgcatcgat
     3721 ccgcatgccg aattcgagcg cgcgcagctg ctccaggctc tcgcgctgct ccaggatgcc
     3781 tcgcggcaga cgaacgaaac gcgcgagtgc ttcgcgctgg taggtgtaca tgccgacatg
     3841 gtggtagtgc ctgcccgcgc cagccgggat ggcggcgcgg ctgaagtaga tcgcccgacc
     3901 aaccgtatcg ccaggcttga ccgcgagcgc gatcttcacc acctggggcg cctgcaactc
     3961 aagcgggtcg ttgatctcgg ttgccagcgt ggcgatggac acatccgcat cccgccggag
     4021 cggtcgcaat gtcgctcgca cggtgaccgg atcgatcgtc ggcagatcgc cctgcaacac
     4081 cacgaccacg tcgtaggcac catccggatc gaccatcccg agcgcggcgt acgcgcggtc
     4141 cgagccggtt ggcagtgccg ggtcggtcag caccgcgcgg ccgccaaggt cctggatcgt
     4201 atcggcaatc ggctggtcac cgcaggcgac gacgacgggg ccgatatcgg cctcgatgcc
     4261 gcgccgccag acatgcgcga tcatcgggct gccattgatg tcggtcagcg ccttgccggg
     4321 tagtcgcgtt gagccgagtc tggtgggaat cacgacgatc gggcgcatcg gaagtctccc
     4381 atcgaaccag caaccgcaag actaccaccc gcgtcagtcg gttgacagcg caccacacca
     4441 ccgctcagtt tcctccatcg cagcgacgac gcgatcgggg ggcatgacaa ccatcagcaa
     4501 cgccatgccg ccgacgcgcg gcatgatcgg actgaccgtg cgcgccgccg aacgcttcgg
     4561 tgtgcaggcg ctgatcgcct gcggcgcgct gttgattgtc gtgccgctgc tgctgacgct
     4621 ctacctcagc gtgttcgacg aaaagctgat cctgtttccc ccgcgcggct acacgctgac
     4681 ctggtatccg gcaatcctgc cgaatttcgg cgacgccatc gtcacgagcc tggagctcgg
     4741 cgcggccgcc gttgtgggca gcctgctgct cggcgtgcct gcgggtatcg gcctggcgcg
     4801 gcatcgcttc cgtggccgag gcgccatctc cacgctgctg ctggcgccac tcaccgtgcc
     4861 gggtatcgcg cttgggcttt cggtctatgt cgggctcgtg gcgatcgatg agcggttcgg
     4921 ttccgctgtc actggctcga tggcgggact ggtgctggcg cacatcatgc tcaccacgcc
     4981 ctgggtcgtg cggctgtgcc tcgccagcct caccaaccat gagcacgccg ccgaggaggc
     5041 cgccgcgagc ctcggcgccg gtccgctgcg cgtgatctgg cgcgtcacct tgccggcgat
     5101 gcgatcgggc atcatcgccg cggcgctgtt cgccttcatc ttctcgttcg agaacctcga
     5161 actggcgctg ttcctgacca gccccggcgt gaccacgctg cccgtcgcgg tactgcaata
     5221 cctggaatac cacatcgacc ccctggtttc cgccgtcgcg gtggcgcaga tcgtggcggt
     5281 ggcggcactg ctgctggtgc tcgatcgctt cgtccggctg ggcgaggtgg tgcagtgagc
     5341 gacgcacacg cgttggcgct ggaagggctg tcgaagcagt tcggcacgca gacggcggtg
     5401 gacgaggtgg atctcgccgt gcggccgggc gagctgatcg tgctgcttgg cccctccggc
     5461 tgcggcaaga cgacgacgct gcgcatgatc gccggctttg tgccgccgag cggcggcgat
     5521 atccgcgtgg acggcagcag cattgtcgcg ctgccagcgc atcgccgcga aatgggcatc
     5581 gtgttccaga gctacgcgct gtttccccac ctgagcgtcg cacgcaacat cgcgttcgga
     5641 ttggagatgc ggcggatggc agcggccgct atagcctcgc gcgtggccga gatgctgcgg
     5701 ctggtgaagc tggaggcctt cgccgatcgc ctgccgcggc agctttccgg cgggcaacag
     5761 cagcgcgtgg cgctggcacg cgcactcgcg atcaatccgc gcgtgctgct gctggatgag
     5821 ccgctgtcca atctcgatgc gtcgctg
//
LOCUS       DATGEM010000316        14134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1401222, whole genome shotgun sequence.
ACCESSION   DATGEM010000316 DATGEM010000000
VERSION     DATGEM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14134
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401222"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1685
                     /locus_tag="VKQ27_10120"
     CDS             141..1685
                     /locus_tag="VKQ27_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89330.1"
                     /translation="MRAALKDEIDASIRGSYEIGGGVTMFDFSASSPLVHMHEQTHRD
                     NVNNTLFGYWITRLADLGERPPAEGAKPANVLLSRAIQGAWDTMEGAATFTEFLNIIC
                     GAVTFDLNAWEAELPADYRRAFALYYRSLKGFARPYPDGDLQLLALSTLNHAVAETVL
                     DGALHSLPALPASLADLTRAFARADLPRKLRRTLTAVHRIDIRGSMKHVENAQWLPDG
                     SDGSRFVRGQVMAIKHALTAYLHPRSKVGMIHDRMHIDLRIAIDAELCRTIADLPPRP
                     FVRRIIGGASTADLGAVSPTWQVARTRVAIGEVAERVRLEQAHPDTFAVGIVILRDPR
                     SDSYTGGVIPIDLVEQENGQGIGLFRESAQFDGTPAEVQALSSELGAEGYFWITPLVV
                     RGDETTAVPYDWEFLSGLAAPLFFFSSEFSEVALTALMAGFGRKPELYATSMRKGGHT
                     FDVVWFKQENATLFWVCGGLVNDRARSMGWADVGGYRATLVPQPEEAALAAAICLVLS
                     DLGKDE"
     gene            complement(1801..2004)
                     /locus_tag="VKQ27_10125"
     CDS             complement(1801..2004)
                     /locus_tag="VKQ27_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89331.1"
                     /translation="MTTTLIDDPVLVRFSKALDEMYGDPHRGSDYDVAAFLHDIRSRD
                     PGIQHEVREGTRRPPRRLVPDFI"
     gene            complement(2013..3488)
                     /locus_tag="VKQ27_10130"
     CDS             complement(2013..3488)
                     /locus_tag="VKQ27_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY89332.1"
                     /translation="MTQDKATRHTHQSFFFESDIFSADRLIARRARPGTTIQEPARAI
                     PVLRECDVLVVGGGPSGTAAAVAAARTGADVVLLERYNHLGGLSTGGLVIWIDRMTDW
                     SGKQVICGIANDLLDRLPKDALAGPPRADWGSKDEATAAYWKDRTAAYHGIVTWSPTL
                     DPERLKLVSQDLVLESGVQLLLHCWGAAPIIEDGAVAGVVFESKEGRLAIRARVTVDC
                     TGDGDIFGRAGAGADTDVEERDIHHCMNTAWIWGGCDMDRWIAFKTSDAAGFSAFMDR
                     GRELCGGLFEKPFVSWRNDVALFMGPRLSGYSAIDVQDLTEVEIRSHRAMARHLEVYR
                     AHAPGFEHAFLMLSAPQIGVRHSRRLPGMARVLRERWGEAEPYPDEIGVSPSLSPKMA
                     PISVPYGCLVPRTVDGLLVAGRHVSCDAASHSFMREIPQCWLTGQAAGAAAGVSVARS
                     VAPRAVPVAEVQAELLRQGAFVRAAAPSPMQPADVPAKMEA"
     gene            3581..3967
                     /locus_tag="VKQ27_10135"
     CDS             3581..3967
                     /locus_tag="VKQ27_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89333.1"
                     /translation="MKQMATCLTDQQEAVTLGSVVAFDGQYGVVWHATSSTLRVLRMT
                     RGMTAVRLSLANEVALHLPVSLGGWFIAYDELVAWPRQQCSVVGEINERCLLKTIDAR
                     SSTKVPRIPAGIVGTFAESAIRSARG"
     gene            4054..4446
                     /locus_tag="VKQ27_10140"
     CDS             4054..4446
                     /locus_tag="VKQ27_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89334.1"
                     /translation="MVPSAGSPIMLKQAIPNVFVRDFEAALAYYAGPLGFRTLFAYGD
                     VPFYAHVARDDAILAIRHVDGPVIDHSAGEDLLSAFIEVSDVNALYQQLRTAGARIQH
                     GPRDEPWGMRSMIVSDLDNNLICFASNL"
     gene            complement(4473..5546)
                     /locus_tag="VKQ27_10145"
     CDS             complement(4473..5546)
                     /locus_tag="VKQ27_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89335.1"
                     /translation="MNDRLLIGTKKGLFELRRARGEWSITATRFLGDPISMLLADPRD
                     GTLFAAEALGHFGVKLQRSRDHGATWQEIPAPAFPESGTEGPSVSYLFCLEPGAADQP
                     GSIWCGTIPGALFLSKDHGETWSLNEALHALPERKDWMGGGFDDAGVASICIDPRDAA
                     HMTIGVSTGGVWSSSDGGTHWTAASQGMRADYFPEPRNQAPEVQDIHRLVQCPAAPDA
                     LWVQHHNGVFRSTDAARSWQEVTTIKPAKFGFAVVVHPHDPNTAWFVPGVKDECRVPA
                     DGRLVVARTRDGAQSFDVLTNGLPQQHAYDLVYRHALAVDDTGTRLAMGSTTGHLWIT
                     EDSGDAWTLVAGHLPPISCVRFG"
     gene            complement(5650..5919)
                     /locus_tag="VKQ27_10150"
     CDS             complement(5650..5919)
                     /locus_tag="VKQ27_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLY89336.1"
                     /translation="MARVSFTANLQRHLSCPDQTVPGSTVRAVLDQVFAAEPRLRSYI
                     LDDQGRLRKHVAIYINGDRIADRLTLTDPIVDSDEVFVFQALSGG"
     gene            complement(5926..6333)
                     /locus_tag="VKQ27_10155"
     CDS             complement(5926..6333)
                     /locus_tag="VKQ27_10155"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89337.1"
                     /translation="MQIQPYLFLDGRAEEAIEFWRKAVGAEVQMLMRWKDCPEKGNSP
                     TPMPDDKVMHASIRIGDSTILASDGNCSGQVKPQGFALSLTAKDPAEAQKLFGALSEG
                     GQVQMPLGKTFFSPAFGMLADKFGVSWMVYVEH"
     gene            6422..7039
                     /locus_tag="VKQ27_10160"
     CDS             6422..7039
                     /locus_tag="VKQ27_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HLY89338.1"
                     /translation="MDVMRRAYHHGDLRRALLEAGIALAREGGPEAVVLREATRRAGV
                     VPNAAYRHFASRDALLQAVRAAALAALAVAMEKELAHARKQSDVARASLRAIGVAYLR
                     FAAREPGLFRTAFTIAGEPETAGEPGWAGRSGMNPFQLLGAALDQMVAAGALPAKRRP
                     GAEYLAWSAVHGLAMLVLEGPLRRMTRKEIQEIGRRVVEMVEKGL"
     gene            complement(7060..7965)
                     /locus_tag="VKQ27_10165"
     CDS             complement(7060..7965)
                     /locus_tag="VKQ27_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY89339.1"
                     /translation="MDRIAALELFVAVVDAGSLSGAARASNLSLPSVSRHITALEEHV
                     GTRLVIRTTRSLAMTEAGRVYYEHARRMLADLNEIEQGLLADASIPAGKLSIWAPTLF
                     GRVFIAPLLARFLADNPRVTIDVTLLDRDFNLVEEGIDLAIRIGPLKDSSLIIRRLGS
                     LLWVVSAAPDYLRRRGTPRTPDDLDDHDCLAFTQPGYEWRFCRDGRASSRQPPARMRA
                     NALDAVVEAAVAGNGLVYAPAWQVQDHVAAGRLRVVLRDYEVPPLPVNAVLSHTRLLS
                     NKVRTLIDFLAAQLNSRDFSSLRSS"
     gene            8107..9096
                     /locus_tag="VKQ27_10170"
     CDS             8107..9096
                     /locus_tag="VKQ27_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89340.1"
                     /translation="MRFGLFGSAEAGCDGSGRGFHGYVDYNVEAEALGYCSSFLVEHH
                     FTGWSQVSATLQLLGWIAARTTTLRVGSAVMVLPWHNPVLLAEQAATLDVLSNGRLDL
                     GVGKGYRHSEFKGFGIPAEEADVRFEEALTILVKSWTTRGRFAYSGHFWTYDDIVVEP
                     APHQQPHPPLWMAAASEASVCRVAERGCNLLLDQYGSVEAHARRVAQFRTEVERRGGT
                     FDPLSVAVARNLYVAKTDADKAEALARYRAMQQRTLAASRDPHHAGGSHILRYDGTDT
                     EPNMLYGTVDEITGQLEGLRRAGIQYLLLNIGGMSRDSLRTFAREIMPVFAVA"
     gene            complement(9106..9996)
                     /gene="rsmI"
                     /locus_tag="VKQ27_10175"
     CDS             complement(9106..9996)
                     /gene="rsmI"
                     /locus_tag="VKQ27_10175"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLY89341.1"
                     /translation="MPDDACDASPEPRQEGPSSLPNGLVLVSTPIGNLGDLTPRARDI
                     LRAADIVLCEDTRHTAKLLAVIGANVKQQPLHEHNEDAAIPTVLRRLREGARIALVSD
                     AGMPLVSDPGYRLVRAALAEGLPVTALPGANAAVMALVLSGLPPLPFLMQGFPPPKRA
                     ARRSSFGQLRAAERAGLAATLIWYEAPHRLAETLSDMADVFGDRHAAIARELTKQFEE
                     VRRGMLTELAGHYAASAPRGEITIVVGPPEQDDHEDLDGQLRTALATSSVKDAAALVA
                     AATGLPRKVVYARALELSKA"
     gene            9981..11258
                     /locus_tag="VKQ27_10180"
     CDS             9981..11258
                     /locus_tag="VKQ27_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HLY89342.1"
                     /translation="MHRRAFVLLLGTTALAGCGGGGYGYGYGGGAFGRYGGAPVPLSG
                     GPPAGPGGPAGQRVAILLPLSGPRADIGQGMLRAAQLAMDAQGAPPLDAKDTGGTPDG
                     AAAAARAAIGDGAGLILGPLTSPETAAVAPVARGANVAVLAFTNDPSQAQPGVWTLGI
                     TPGQQVRRLVAAAQGQGKTQFAALLPDSDFGHVMGDALTRATAADSLPPPNLRFHSPG
                     MQSINQTTRDLSDYASRGGPIYSQIKSLRESGTSEGRKQAQEMARTAVVPPPSFNVLL
                     LADTGESLAEVASVLPYYDVNRSQVQIIGPSLWASPSSGSGQVPGAWYAAPDPAARAA
                     FDQAYSAKYGGPAGPLGELAYDAASISRVLGSRGDYSVGALTQDAGFLGADGWFALLS
                     DGQVRRALAVFVIERGGPQMLEPAPQSGNVPGA"
     gene            complement(11261..13129)
                     /locus_tag="VKQ27_10185"
     CDS             complement(11261..13129)
                     /locus_tag="VKQ27_10185"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HLY89343.1"
                     /translation="MRFALGLLLFAALGLAGIPARAWDDFQIIEWQHRDAAQLATLKQ
                     LGVTAATVVANRDGTGTPVEQQIAPMLANGLRWYVENIATDYYSAYHRWLPPHPVNWR
                     FLDAQQRYRANPDDESAVWRDPSLADPMWQRRIRDRLIATVRGQMRYRPLYYSLGDET
                     GIADLSAFWDFDLSPGSLAGMRVWLRQTYGSLAALNAEWGTRFADWNDVHPATTREAM
                     RRTDDNYSSWADFKAWMDVAFARALRMGTDAVHHAEPTALAAIEGVQVPGWGGYDYTL
                     LVNAVDVLEVDDLPLAHSLNPHVTTLTTSFDAQPRGLHKLWRNLLAGSRGVILWDSDG
                     TIVRPDAALGERGQAYAETFREIRGGIGHLLLNSEPHSDPIAILYSPASFRVQWMLEQ
                     KPKGDAWMNRDAEAELSGDAAREAMWAYASAVTHQGLQPMFVSPAMIEHGALRQRGIK
                     LLILPHAIALSRAEAQQIRDFRGAVIADVQPGKFDEHGRRLDHPLLDAGTSRMVAPDQ
                     FRNAASEGCQPPLPVIASVAKQSRPDCFAALAAISPSIHVDAADVTVHIWRDGKAQIV
                     GVQRDFAPDAPDEQVTLNWPGQRTILDLRTNRTLGRSDHLTARLDAVYPLLLSLTP"
     gene            complement(13126..13824)
                     /locus_tag="VKQ27_10190"
     CDS             complement(13126..13824)
                     /locus_tag="VKQ27_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLY89344.1"
                     /translation="MASITVRATDGSGSFSAHIAEPASKPTGVVVLIQEIFGVNQAMR
                     DIAAWVADIGFIAVCPDLFWRIEPGIDITDKSEAEWKQAFELFGKFDQAKGIEDLKAT
                     VAAARKLPGANGKVATMGYCLGGRLAFMMAEQSDADANISYYGVGLDSLLNDLGKVTK
                     PLLVHIADKDEFFPAEGRAKVVEATKGRAHIACHVYPNADHAFARVGGVHWDGRSATI
                     ANGRSTEALVAAIG"
     gene            complement(13928..>14134)
                     /locus_tag="VKQ27_10195"
     CDS             complement(13928..>14134)
                     /locus_tag="VKQ27_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89345.1"
                     /translation="VACLGIGILMSHLTLLRYKINAWSTAPAIRYSLSAHIQPLLCPH
                     AKLTTNVKLNDSTPLVTASCPPLW"
BASE COUNT         2417 a         4239 c         4968 g         2510 t
ORIGIN      
        1 acagcagcgg cgttgggcgc gttatccatc atgtctgtat atctaacatc agagcaggcg
       61 gcgaggcaaa cgattccttt tggtgatggg aacgtggatc tgattatgta ttcctgccta
      121 ctccggcgcg ggagacgcga atgcgggcag ccctgaagga cgagatcgac gcctcgattc
      181 gaggcagcta cgaaatcggc ggtggcgtca ccatgttcga tttcagcgcg tcgagcccat
      241 tggtgcatat gcacgagcag actcaccggg acaacgtcaa caacactctg ttcggctatt
      301 ggatcacgcg cctggcggac ctcggtgaga ggccgcctgc cgagggtgcg aagcccgcca
      361 acgtactcct gtcgcgagcc attcagggag cgtgggatac gatggaagga gccgcgacgt
      421 tcaccgaatt cctgaacatc atctgcggcg cagtgacgtt cgatctgaat gcctgggagg
      481 ccgaactccc ggccgactac cgccgggcct tcgcgctcta ctatcgaagc ctgaagggct
      541 ttgcgaggcc gtatccggat ggtgatttgc agctacttgc actgtccacg cttaatcatg
      601 cagtggccga aacggtgctc gacggtgccc tccattccct gcctgctctg cctgcgagcc
      661 tggccgatct gaccagggcg tttgccagag ccgatctgcc gcggaaattg cgtcgcacgc
      721 tgacggcggt ccatcgtatc gacatacgtg gcagcatgaa acatgtcgag aatgcgcaat
      781 ggctgccgga cggcagcgat gggtcgcgat ttgtgcgagg ccaggtgatg gccatcaaac
      841 acgcgctgac ggcgtatctt catccacgct cgaaggttgg aatgatccac gatcggatgc
      901 acatcgatct gcggatcgcc atcgatgccg agctttgccg cacgattgcg gatctgccgc
      961 cgcgtccctt cgtgcggcgg atcatcggcg gtgcatccac tgctgatctg ggcgccgttt
     1021 ctccgacctg gcaggtggcg cgcacgcgcg tggcgatcgg cgaggtcgcc gaacgtgtcc
     1081 ggctggaaca ggcgcatccc gacacattcg cggtcggtat cgtcatcctg cgcgaccctc
     1141 gctcggattc ttacaccgga ggcgtcatac ccatcgacct ggtcgagcag gagaatgggc
     1201 aggggatcgg attgttccgg gagtctgcgc agttcgatgg cacgcctgcc gaggtgcagg
     1261 cgctttccag cgagctgggg gcggaaggtt acttctggat cacgccgctt gtggtccggg
     1321 gcgacgagac gacggccgtg ccttacgact gggagttcct gtcaggactg gccgcgccgt
     1381 tgttcttttt cagcagcgag ttttccgagg tcgctctcac agcgctgatg gcgggcttcg
     1441 gccggaaacc ggagctgtac gcgacatcga tgcgaaaggg gggccacacg ttcgatgtgg
     1501 tctggttcaa gcaggagaac gccaccttgt tctgggtgtg cggcggcttg gtgaacgacc
     1561 gcgcgcgcag catgggatgg gcggacgtgg gcggctaccg cgccacgttg gtacctcagc
     1621 cggaggaggc tgcgcttgct gctgccatct gcctggtgtt gtcggacctg gggaaggatg
     1681 aatgagccgg gcagcggaaa gctggctgcg tgatcgatgt cggcgcagag catcggcata
     1741 atccaacaca aagctcgcga agatcacgaa ggacattcca ggcgctattc gtccggtctc
     1801 ttaaatgaaa tccggaacaa gtctcctcgg cggtcgtcgc gttccttcgc gaacttcgtg
     1861 ttgaatccca ggatcgcggc ttcgaatgtc gtgcaggaag gccgcgacgt cataatcaga
     1921 gccgcggtgc ggatcaccgt acatttcatc cagcgccttg ctgaagcgga ccaggacagg
     1981 atcatcgatc agcgttgtgg tcatagcgct tttcacgcct ccatcttggc aggcacgtcg
     2041 gctggctgca tcgggcttgg cgccgctgcc ctgacgaacg ccccctgccg caacagctcc
     2101 gcctgcactt ccgctaccgg caccgcgcgc ggggccacgc tccgcgccac agacacgccc
     2161 gctgccgcac cagcagcctg gccggtcagc cagcactgcg ggatctcgcg catgaacgaa
     2221 tggcttgcgg cgtcgcagga cacgtggcga ccggcgacca gcagcccatc caccgtgcgc
     2281 ggcaccaggc agccatacgg caccgatatc ggtgccatct tcggcgacag cgagggcgac
     2341 acgccgatct cgtcggggta aggttcggcc tcgccccagc gctcgcgcag cacgcgcgcc
     2401 ataccgggca gacggcgcga atggcgcacg ccaatctgcg gggcggacag catcaggaag
     2461 gcgtgctcga agccgggggc gtgggcgcga tacacctcga gatgacgcgc catggcgcgg
     2521 tgcgagcgga tttccacctc ggtcaggtcc tgcacgtcga tcgcggaata gccggagagg
     2581 cgtgggccca tgaatagcgc cacgtcattg cgccaggaga cgaacggctt ctcgaacaag
     2641 ccgccgcaca gctcgcgacc gcgatccatg aaggcggaga agcctgccgc gtcggaggtc
     2701 ttgaacgcga tccagcggtc catgtcgcaa ccgccccaga tccaggcggt gttcatgcaa
     2761 tggtggatgt cgcgctcctc gacgtcggtg tccgcgcctg ctccggcgcg gccaaaaatg
     2821 tcgccatcgc cggtgcagtc cacggtgaca cgggcgcgga tcgcgaggcg gccttccttg
     2881 ctctcgaaca ccacgccggc gacggcgccg tcctcgatga tcggcgccgc gccccagcag
     2941 tgcagcagga gctggacgcc gctttcgagg accaggtcct ggctgacgag cttcaggcgc
     3001 tcgggatcga gcgtggggga ccaggtgacg atgccgtggt aggcggcggt gcggtccttc
     3061 cagtaggcgg cggtggcttc gtccttactg ccccagtcgg cgcggggcgg tccggcgagc
     3121 gcgtctttcg gcagccgatc gagcaggtcg ttggcgatgc cgcagatcac ctgcttgccg
     3181 gaccagtcgg tcatgcggtc gatccagatg actaggcctc cggtggagag gccgcccagg
     3241 tggttgtagc gctccagcag cacgacgtcg gcgccggtgc gggccgcggc gaccgcggcg
     3301 gcggtgcccg aggggccgcc gcccaccacc agcacgtcgc attcgcgcag cacggggatg
     3361 gcgcgggcgg gttcctggat ggtggtgccg ggacgggcgc ggcgcgcgat caggcggtcc
     3421 gccgagaaga tgtcggactc gaagaagaac gactggtgag tgtggcgggt ggccttgtcc
     3481 tgggtcacgg tggttctcct tcaaatctca cacacttgtg agtagcagac ttgcctcggc
     3541 aagcgcagcg tccgccgcct taagccaagg gaggttacgc atgaagcaga tggcaacatg
     3601 cctgaccgat cagcaagaag cagtaacgct cggttcggtg gtggccttcg atggtcagta
     3661 tggtgtggtg tggcacgcaa ccagctctac gctgcgcgtg ctgcggatga cgcgcggcat
     3721 gacggcggtg cggctgtcac tggcaaatga agtcgcgttg catttgccgg tcagcctcgg
     3781 cggctggttc attgcttatg acgaactggt ggcgtggccg cgtcagcaat gcagcgtcgt
     3841 cggcgagatc aacgaacgct gcctcctgaa gacaatcgat gcgcgcagct cgacgaaggt
     3901 gccgcggatt cccgcaggca tcgtcggaac attcgctgag agcgcgatcc gaagcgcacg
     3961 cggctgatcg gcagtggatg gccggcggcc agagcattgg ccgccggtat tttctgcagg
     4021 catcgagcac ggtaaactac aagcagatca cagatggtgc cgagcgctgg ctcgccgatc
     4081 atgctgaagc aggcgatccc gaacgtcttc gtcagggatt tcgaggcggc tctcgcatac
     4141 tatgctgggc cactcggctt tcgcacgctg ttcgcctatg gcgatgttcc gttctatgcg
     4201 cacgtggcgc gtgacgacgc gatattggcc atccgccacg tcgacgggcc ggtcattgac
     4261 catagcgcgg gcgaagatct gctcagcgcg ttcattgagg tgtcggacgt caacgcgctg
     4321 tatcaacaat tgcgaaccgc tggtgcgcgc atccagcacg ggccgcgcga tgagccttgg
     4381 ggcatgcgga gcatgatcgt cagcgatctc gacaacaacc tgatatgctt cgcgtccaat
     4441 ctgtagcggc ttggatgccg gaacgggtgt gatcagccga aacgcacgca gctgatcggc
     4501 ggcaggtggc cggcgaccag tgtccaggcg tcgccgctgt cctcggtgat ccagagatgg
     4561 ccggtggtcg agcccatcgc gagccgcgtg ccggtgtcat ccaccgcaag cgcgtggcga
     4621 tagaccagat cgtaggcgtg ctgctggggc aacccgttgg tgaggacgtc gaaggattgg
     4681 gcgccgtcac gggtgcgggc gacgacgagg cggccatccg ctggcacgcg gcactcgtcc
     4741 ttcacgccgg gcacgaacca ggcggtgttc ggatcatgcg gatgcaccac gacggcgaaa
     4801 ccgaatttcg caggcttgat cgtggtgacc tcctgccagg aacgcgcggc gtcggtggag
     4861 cggaagacgc cgttgtggtg ctgcacccat agcgcgtcgg gggcagcggg gcattgcacc
     4921 aggcggtgga tgtcctgcac ttcgggcgcc tggttacggg gttcggggaa gtagtcggcg
     4981 cgcatccctt gggaggcggc ggtccagtga gtgcctccgt cgctggagga ccagacgcct
     5041 ccggtcgaga cacctatcgt catgtgggcg gcgtcgcggg gatcgatgca gatggaagcg
     5101 acgccagcat cgtcgaagcc cccgcccatc cagtccttgc gttcggggag ggcgtgcagg
     5161 gcctcgttca gcgaccaggt ctcgccgtgg tctttcgaca ggaagagcgc gccggggatg
     5221 gtgccgcacc agatcgagcc tggctgatcg gctgcgccgg gctcgaggca gaagaggtag
     5281 gagacgctgg ggccttcagt gccggactca gggaacgcgg gcgcggggat ttcctgccag
     5341 gtcgcgccat gatcgcgcga gcgctgaagc ttgacgccga aatggccgag ggcttcggcg
     5401 gcgaagaggg tgccgtcgcg tggatcggca agcagcatgg agatgggatc gccgaggaag
     5461 cgcgtagcgg tgatcgacca ctcgccacgt gcgcggcgca gttcgaacag gcccttcttg
     5521 gtgccgatca gcagacgatc gttcatgcta tggctcccga ttgggttgtc attgcgagga
     5581 acggagcaaa gctcgcgcgg gattgcttgg tcacttcgtt cctcgcaatg acaaaggtat
     5641 gggcatgggt tatccgccgg acagggcctg gaacacgaat acttcgtcgg agtcgacgat
     5701 tggatcggtg agggttagcc gatcggcgat gcggtcgccg ttgatgtaga tcgctacatg
     5761 cttacgcagg cggccctggt cgtcgagtat atacgagcgt aaacgcggct cggcagcgaa
     5821 cacctggtcc agcacggcac gcactgtgct gcccgggacc gtctggtccg ggcagctcag
     5881 gtggcgttgc aggttcgcgg tgaacgagac gcgagccacg atccctcaat gctcgacgta
     5941 gaccatccat gatacgccga acttgtcggc aagcataccg aatgccggcg aaaagaacgt
     6001 cttgccaagc ggcatctgca cctggccgcc ttcggacaac gcgccgaaca acttctgggc
     6061 ttcggccgga tccttggcgg tcagcgacag cgcaaaacct tgtggcttga cctggccact
     6121 gcaattgccg tccgaagcca ggatcgttga gtcgccgatg cggatgctgg cgtgcatcac
     6181 tttgtcatcc ggcattggtg tcggtgagtt gcccttctcg gggcagtcct tccagcgcat
     6241 cagcatctgc acttcggcgc cgacggcctt gcgccagaac tcgatcgctt cctcggcgcg
     6301 gccgtccagg aacaggtagg gttgaatctg cattgatctt ctcctccacg atgtctacgc
     6361 tgtccacatg gagatagcac atggtgtgga tggtgtccac atgctcgcgg agcggtgatc
     6421 gatggatgtg atgcgccgcg cgtatcacca cggcgatctg cggcgtgcgc tgctggaggc
     6481 ggggatcgcg ctggctcggg aaggcggtcc tgaggcggtc gtgctgcgcg aggcgacgcg
     6541 gcgcgcgggt gtggtgccta acgcggccta ccggcacttc gcaagccgcg atgcgctgct
     6601 gcaagcagtg cgggcggcgg cgctggcggc cctggccgtg gcgatggaga aagaactggc
     6661 gcacgcgcgg aagcagtccg atgtggcgcg cgccagcctg cgggccatcg gcgtcgcgta
     6721 tctgcgattt gccgcgcggg agcccgggct gttccgcacc gcattcacga ttgccggcga
     6781 gccggagaca gcgggcgagc cgggttgggc ggggcgcagc gggatgaacc cgttccagct
     6841 actcggggcc gcactggacc agatggtggc agccggcgcg ctgccggcga agcgacggcc
     6901 cggggcggaa taccttgcgt ggtcggccgt gcacggtttg gcgatgctgg tgctcgaagg
     6961 gccgctgcgg cgaatgacgc gcaaggaaat ccaggagatc gggcggcgcg tggtggagat
     7021 ggtggagaag gggctgtaga gcctcgcgaa gctgcccgct caggatgagc ggagggaaga
     7081 gaaatcgcgt gaattcagct gcgccgccag gaagtcaatg agggtgcgga ctttgttcga
     7141 aagcagcctc gtatggctca gcacagcgtt gaccggtaat ggcggcactt cataatcgcg
     7201 cagaacaact cgcagccttc cagcggcaac atggtcctgt acttgccagg caggagcata
     7261 gacgagaccg ttgccggcaa cggctgcttc gacgacggca tcgagcgcgt tggcgcgcat
     7321 gcgcgcgggt ggctggcggc tggacgcgcg tccatcgcgg cagaagcgcc actcatagcc
     7381 tggttgggtg aatgccagac agtcgtggtc atcgaggtcg tccggtgtcc tcggcgtgcc
     7441 ccggcgcctg agatagtcgg gtgcggcgct gacgacccat aacaagctgc ccagacggcg
     7501 gatgatcagg ctcgagtctt tcagcggccc gatccggatc gccaggtcga ttccctcctc
     7561 gaccaggttg aagtctcggt cgagcagcgt gacgtcgatg gtgacgcgcg gattgtccgc
     7621 caggaaacgg gcgagcaaag gagcaataaa tacgcggcca aacaacgtcg gcgcccagat
     7681 gctcagtttc cccgctggaa tgcttgcgtc ggcgagcagg ccctgttcga tctcgttgag
     7741 atcggccagc atgcgcctgg catgttcgta atacacgcgg cctgcctcgg tcattgccag
     7801 cgagcgggtc gtgcgtatca cgaggcgggt tccgacatgc tcctccagtg ccgtgatgtg
     7861 gcgggacacc gagggcagag acaggttcga ggcacgcgcg gcgccggaca agctgcccgc
     7921 gtcaacaacc gctacgaaca gttccaaagc agctatgcga tccatgacgt cctttcgttc
     7981 aacggcatga taacggcctg gtacgtccat tgcgaaagaa tctgaaagga tgactttccg
     8041 cggttgcggc taccggtgaa gggggcagtc gccctacctc tgccggcgca ggcgaggagc
     8101 ggataaatgc gatttgggct gttcggcagt gccgaggctg gctgtgacgg ttccggccgg
     8161 gggttccacg gctacgtcga ttataatgtc gaggccgaag cactgggcta ttgcagcagc
     8221 ttccttgtcg agcaccattt cacaggctgg tcgcaagtct cggcgacgct gcaattgtta
     8281 gggtggattg cagcacggac cacgacattg cgggtcggtt cggctgtgat ggtgctgccg
     8341 tggcataacc cggtgctgct cgctgagcag gcagcgacgc tggatgtcct ctcgaatggc
     8401 cggctcgatc tcggcgtcgg caaaggctat cggcacagtg agttcaaagg cttcggcatt
     8461 ccggccgagg aagccgatgt gcgatttgag gaagcgctga cgatcctggt gaagtcgtgg
     8521 accaccagag ggcgatttgc ctacagcggc cacttctgga cgtatgatga cattgttgtc
     8581 gagccagcac cgcaccagca gccgcatcct ccgctgtgga tggccgcggc cagcgaagct
     8641 tcagtctgcc gcgttgccga acgcggctgc aacttgctgc tcgaccaata tggatctgtc
     8701 gaggcacatg cccggcgggt tgcgcagttc cgcactgagg tcgagcgtcg tggcggcacg
     8761 ttcgatcctc tgagcgtcgc tgttgcgcgc aacctctacg ttgcgaagac cgacgcggac
     8821 aaggcggaag cgttggcgcg ctaccgcgcc atgcagcagc ggacactcgc ggcgtcgcgc
     8881 gacccgcatc acgcgggcgg ctcacatatt ctgcgctatg atggcacgga cacggaaccg
     8941 aacatgctct atggcacggt ggatgagatc accgggcaac tcgaggggct gcgccgtgcc
     9001 gggatacaat acctgctgct caatattggc ggcatgtccc gcgacagcct gcgaacattc
     9061 gcgcgcgaga tcatgccggt ttttgccgtg gcgtgaacgg accgcttagg ccttggagag
     9121 ctccagggca cgggcataga ccaccttgcg cggcagaccg gtggcggcgg cgaccagtgc
     9181 ggctgcgtcc ttgacgctgc tggtggccaa ggccgtgcgc aactgaccgt cgaggtcttc
     9241 gtggtcgtcc tgttcgggtg ggccgacaac gattgttatt tcaccgcgag gcgcgctggc
     9301 ggcgtagtgc ccggcaagct cggtcagcat gccgcggcgt acttcttcga attgcttggt
     9361 cagttcgcgt gcgatggccg catgacggtc accgaacacg tcggccatgt ccgacaaagt
     9421 ctcggcaagg cggtgcggtg cctcgtacca gatcagagtc gccgcgaggc cggcgcgctc
     9481 ggccgcccga agctggccga aagaggagcg gcgcgcggcc cgcttcggcg gtgggaagcc
     9541 ctgcatgagg aacggcagcg gcggcaggcc ggacagcacc agggccatca cggcggcgtt
     9601 cgctccgggt agggcggtca ccggcaatcc ctcggcgagg gctgcccgga cgagccgata
     9661 gccggggtcg gagaccaacg gcatgccggc atcggagacc agcgcgatcc gggcaccttc
     9721 gcgcaggcgg cgcaagacgg tggggatcgc ggcatcttcg ttgtgctcgt gcagcggctg
     9781 ctgcttcaca ttcgcgccga tcacggcaag gagctttgcg gtatggcgcg tgtcctcgca
     9841 gagaacgatg tcggcagccc gcaggatgtc acgcgcgcgt ggtgtcagat cgccaaggtt
     9901 gccgataggc gtggaaacca gcacaagacc gttcggcagg gaggatggtc cctcctgtcg
     9961 cggctcagga gatgcgtcgc atgcatcgtc gggcattcgt gctccttctt ggcacaacgg
    10021 ccctcgccgg ctgcggtggg ggcggctacg gatatggtta cggcgggggc gcctttggtc
    10081 gctatggcgg cgcgccggtg ccattgtccg gaggaccgcc cgccggtcca ggggggccgg
    10141 ccgggcagcg tgtcgcgata ctgctgccgc tgtcggggcc gcgtgccgat atcgggcagg
    10201 gcatgttgcg ggcggcgcag cttgcgatgg atgcgcaggg cgctccgccg ctcgacgcga
    10261 aggataccgg agggacaccg gatggtgcag ccgccgcggc gcgcgccgcg atcggcgatg
    10321 gagccgggct gattctgggg ccgctgacct cgccggagac ggccgccgtg gcaccggtcg
    10381 cccgtggggc caatgtcgcg gtgctggcgt tcaccaacga cccgtcgcag gcccagccag
    10441 gggtatggac gctcgggatc acgccgggcc agcaggtgcg gcgcctggtg gcggcggcgc
    10501 aggggcaggg caagacgcag ttcgccgcgt tgctgccgga tagcgatttc ggccacgtga
    10561 tgggcgatgc gctgacgcgc gccactgccg ccgacagcct gccgccgccg aacctgcgct
    10621 ttcattcgcc gggcatgcaa tcgatcaacc agaccacgcg cgacctgtcg gactatgcca
    10681 gccgcggcgg accgatctac tcgcagatca agtcgctgcg ggagtccggc acgtccgagg
    10741 gacgcaagca ggcgcaggag atggcccgga ccgccgtggt gccgccaccg agcttcaacg
    10801 tgctgctgct ggctgatacc ggcgagtcat tggctgaggt ggcgtccgtg ctgccgtatt
    10861 acgacgtgaa ccgatcgcag gtgcagatca tcgggccgtc attatgggcg tcgccatcga
    10921 gcgggtcggg gcaggtcccg ggggcatggt atgcagcgcc cgatccggcg gcgcgcgcgg
    10981 cattcgatca ggcgtattcg gcgaaatacg gtggcccggc ggggccgctg ggggagttgg
    11041 cgtacgacgc ggcctcgatc tcgcgcgtgc tggggagccg gggcgattac tcggtgggag
    11101 cgttgaccca ggatgccggg ttcctcggcg cggacggatg gttcgcgctg ctctcggatg
    11161 ggcaggtgcg gcgcgcgctg gcggtgttcg tgatcgaacg cggagggccg cagatgctgg
    11221 agccggcacc gcaatcgggg aatgtgccgg gcgcctgagg tcagggcgtg agcgagagaa
    11281 gcagcgggta gaccgcgtcg agtctggcgg tgagatggtc gctgcgaccg agcgtgcggt
    11341 tggtgcggag gtcgaggatc gtgcgctgac ctggccagtt aagggtgact tgttcgtcag
    11401 gtgcgtcggg ggcgaagtcg cgctgcacgc cgacgatttg cgctttaccg tcacgccaga
    11461 tgtggacggt gacgtctgcc gcatcgacgt ggatcgatgg ggagattgct gcgagcgcag
    11521 cgaagcaatc cgggcgagat tgcttcgcta cgctcgcaat gacagggaga ggcggctgac
    11581 agccctcgga tgcggcgttg cggaactggt caggagcgac catccgggat gttccggcat
    11641 ccagcagcgg gtggtccagg cggcggccat gttcgtcgaa cttgccgggc tgcacgtctg
    11701 cgatcactgc tccgcggaag tcacgtatct gctgcgcctc ggctcgcgac agagcgatcg
    11761 cgtgcggcag gatcagcagc ttgatgccgc gctggcgcag tgcgccgtgc tcgatcattg
    11821 ccggtgagac gaacattggt tgcaggccct ggtgggtcac tgcggaggca taggcccaca
    11881 tcgcctcacg tgcggcatcg ccggacagtt ctgcctcggc gtcgcggttc atccatgcat
    11941 cgcctttcgg cttctgctcc agcatccatt gcacgcgaaa gctggcgggc gagtagagaa
    12001 tggcgatcgg atccgagtgc ggttcgctgt tgaggagcag gtgaccgatg ccgccgcgga
    12061 tctctcggaa tgtttcggcg taggcctggc cgcgttcacc gagtgccgca tcgggccgga
    12121 cgatggtgcc atcgctgtcc cacaggatca cgccacggct gccggcgagc agattgcgcc
    12181 atagcttgtg gagaccgcga ggctgcgcgt cgaatgacgt ggtgagcgtg gtgacgtgcg
    12241 ggttgagcga atgcgccagg ggaagatcgt ccacctccag cacatcgacg gcattcacca
    12301 gcaacgtgta gtcgtagcca ccccagcccg gaacctgaac gccctcgatc gccgcgagcg
    12361 ccgttggttc ggcgtgatgc accgcatcgg tgcccatgcg gagcgcgcgg gcgaacgcta
    12421 cgtccatcca cgctttgaaa tcggcccagg aggaataatt gtcgtcggtg cggcgcatcg
    12481 cttcgcgcgt ggtcgcgggg tgtacgtcgt tccagtccgc gaaccgggtt ccccactcgg
    12541 cgttcagtgc ggcgagtgat ccatacgtct ggcgcagcca gacccgcatg ccggcaagcg
    12601 atcccggcga cagatcgaaa tcccagaaag cagagagatc ggcaatgccg gtctcgtcgc
    12661 cgagactgta gtagagcggc cggtagcgca tctgcccgcg caccgtggcg atcagccgat
    12721 cgcggatgcg cctttgccac atcggatcgg cgaggctggg atcgcgccag acagcggact
    12781 cgtcatcggg gttggcgcga tagcgctgct gggcatcgag gaaacgccag ttcaccggat
    12841 gtggcggcaa ccagcgatga taggccgagt agtagtcggt cgcgatgttc tccacgtacc
    12901 aacgcaggcc attcgcgagc atgggcgcga tttgctgctc gaccggcgtg ccggtgccgt
    12961 cgcggttggc gacaaccgtg gcggcggtga cgccaagctg cttcagcgtg gcaagttgcg
    13021 cggcgtcgcg atgctgccac tcgatgatct ggaagtcgtc ccacgcgcgc gcggggatgc
    13081 ccgcgaggcc gagtgccgcg aacagcagca gcccgagggc gaacctcacc cgatcgccgc
    13141 gaccagcgct tctgtgctgc ggccgttggc gatcgtggcg gagcggccat cccaatgaac
    13201 gccgccgacg cgggcgaagg cgtgatccgc attcggatag acatggcagg cgatgtgcgc
    13261 gcggcccttg gttgcctcaa ccaccttggc gcggccttcg gctgggaaga actcgtcctt
    13321 gtcggcgatg tgcaccagca gcggctttgt caccttgccg agatcgttca gcaggctatc
    13381 cagtccgaca ccgtaatagg aaatgttggc gtcagcgtcc gactgttccg ccatcatgaa
    13441 tgcgaggcgg ccgcccaggc agtaacccat ggtggcgacc ttgccgttcg caccaggcag
    13501 cttgcgcgcg gcggcgacgg tcgccttcag gtcttcgatg ccttttgcct ggtcgaactt
    13561 gccgaacagc tcgaaggcct gcttccactc ggcttccgat ttgtcggtga tatcgatgcc
    13621 gggttcgatg cgccagaaca ggtcggggca gaccgcgatg aagccgatat cggcgaccca
    13681 tgccgcgatg tcgcgcatgg cctggttcac gccaaagatc tcctggatca gcacgaccac
    13741 gccggttggc ttgctcgccg gctcggcgat atgagctgag aagctgcctg atccgtcggt
    13801 ggcccggact gtgatacttg ccatgacgcg ctctccctgc tgatgcgcga gaggttatcg
    13861 cgccctggcc cgactgtcat tggaagccgg tttcattaga gcgcgcccgt ccattttcgg
    13921 cgggccgtca ccaaagcggt gggcaactcg cggtgacgag gggggtggag tcgttgagct
    13981 ttacattcgt agtgagcttg gcgtgcggac agagaagcgg ctggatgtgg gccgagaggg
    14041 agtaccggat ggcgggggcg gtgctccagg cgttgatttt gtagcgcagc aaggtcaggt
    14101 gagacatcag aatgccgatg ccgagacagg cgac
//
LOCUS       DATGEM010000317         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1441242, whole genome shotgun sequence.
ACCESSION   DATGEM010000317 DATGEM010000000
VERSION     DATGEM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441242"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VKQ27_10200"
     CDS             complement(<1..359)
                     /locus_tag="VKQ27_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLY89346.1"
                     /translation="MIDQLKQNNRAWAEGKLAVDPNFFKRLEAQQKPEYLWIGCSDSR
                     VPANEIGGLAPGELFVHRNVANLAPPQDANYLSVLQFAVDVLKVKHILVVGHYGCGGV
                     SAAVDGKRRGLVDHWLHP"
     gene            594..1817
                     /locus_tag="VKQ27_10205"
     CDS             594..1817
                     /locus_tag="VKQ27_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY89347.1"
                     /translation="MSDPTDERVELDAIVAQYRAELARLNYVRGSINVYLRSIRRLFR
                     LMEEQGVALGDLTPDVAADLVRRAQWRCDRQQYAVFIVKRFVEYLAAQGIARPPAPPT
                     AAAMARAALRRDYEDYLLRQRGLSDRTIASCWYVADRFLTFRFGDNDVDFGRIVPGDI
                     VAFLQRLTGRKTPYRDKTQPTHLRNFFQYLFKEGLTATNLALCIPKVAQRYAARLPRH
                     LAPEEVESVLASVRSDPRFGRRNHAMILLLARLGLRAPEVIAIQLDDIDWRAGELVVR
                     GKGQRHDRVPIPPDVGEALADYIRHDRVSASRALFVSTRAPRGPFKDGQMLNAILKDA
                     FARCGVKPPCPYVGSHVLRHSLATNLVRNGASLDEVRDVLRHRSRASTLIYAKVDIEG
                     LRSIAQTWPSTGGAQ"
     gene            1814..>2339
                     /locus_tag="VKQ27_10210"
     CDS             1814..>2339
                     /locus_tag="VKQ27_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY89348.1"
                     /translation="MSLRQELDRYLTIRRSLGYDLRTSERILRRFIAFAESQSTDHIS
                     TDLFVKWQGVFGQASRQTWAARLCIVRLFAQWLHNLDPAHEIPPQGLIPNRSRRSRPH
                     IYSEEEIAKIVAAAAELPSVYGMRGLTYATLFGLIAMTGLRISEALALNVDDVDLDLG
                     VLTIRRGKGGKARFV"
BASE COUNT          406 a          744 c          736 g          453 t
ORIGIN      
        1 ggatgcagcc aatggtcgac gaggccgcgc cgcttgccgt ccaccgccgc gctcacgccg
       61 ccgcagccat aatgtccgac caccaggatg tgcttcacct tgagcacgtc gacggcgaac
      121 tgcagcaccg acaggtagtt cgcgtcctgc ggcggggcga ggttggcgac attgcggtgg
      181 acaaacagct caccgggggc caggcctccg atctcgttgg cgggcacccg gctgtcggag
      241 cagccgatcc agagatattc gggcttctgc tgagcctcca gccgcttgaa gaagttgggg
      301 tcgacggcga gcttgccctc ggcccaagca cggttgttct gcttgagttg gtcgatcatg
      361 gccttcgtta gcactccgtt tgtgctgccg agccggaccg gaatttgccg ccttgtcggg
      421 gcagcagggc gatcacagcc cggttgtgat ctcgactgtg ccgagtgccg cctatcgcgg
      481 gcgcccagtt gttcaaccgt caattctggc cgtgcggcgt tcggcataat gctgatcgat
      541 tactatagca gcggcgcggc atccttggct cctcaacaac aaggagcaag gacatgtccg
      601 atccgacaga tgagcgggtg gagctcgacg cgatcgtcgc ccaatatcgc gccgagctgg
      661 cacggttgaa ctacgttaga ggttcgatca acgtctatct gcgctcgatc cggaggcttt
      721 tccggctgat ggaagagcag ggcgtggcac tcggcgacct gacgccggat gtcgcggccg
      781 atctggttcg tcgagcacag tggcgctgcg accgccagca atatgctgtc ttcatcgtca
      841 agcgcttcgt cgagtatctg gcggcgcaag ggatcgcgag gccaccggcg ccgccgacgg
      901 cagcggcgat ggcccgcgcc gccctgcggc gcgattacga ggattatctt cttcgccagc
      961 gggggctcag cgataggacg atcgcaagct gttggtacgt cgctgaccgg ttcctgacct
     1021 tccgcttcgg cgacaatgat gtcgacttcg gtcggattgt gcccggtgat atcgtggcgt
     1081 tcctccagcg gctgacgggg cgcaagacgc cgtatcggga caagacccag ccgacacacc
     1141 tgcgcaactt cttccagtac ctcttcaagg aggggctgac cgccaccaat ctggcgctct
     1201 gcattccgaa ggttgcgcaa cgttatgcgg cgcggctgcc ccgtcatctg gcgccggaag
     1261 aggtggaatc tgttcttgct tctgtgcgca gcgatccccg cttcggacgg cgcaatcatg
     1321 cgatgatcct cctgcttgcg cgccttggcc tgcgagctcc agaggtgatt gccatccagc
     1381 tcgacgatat tgattggcga gccggcgagc tggtcgtgcg tggcaagggg cagcggcatg
     1441 atcgagtgcc gatcccaccc gacgtgggcg aagcgctggc cgattatatc cgccatgacc
     1501 gcgtgtccgc ctcgcgcgct ttattcgtca gtaccagggc gccacgcggc cccttcaaag
     1561 atgggcagat gctcaacgcc atcctcaagg atgcgttcgc ccgctgcggg gtgaagcccc
     1621 cgtgtcccta tgtcggatcg catgtcctgc gccatagcct tgcgacgaac ctggtgcgca
     1681 acggggcatc gctggatgaa gtgcgcgatg tgttgcgcca tcgttcccgt gcttccaccc
     1741 ttatctacgc caaggtggat atcgagggcc tccgctcgat cgcccagacc tggccgagca
     1801 cgggaggcgc gcaatgagtc tccgccaaga actcgatcgg tacctcacga ttcggcgcag
     1861 cctgggctac gacctccgca cgagcgagcg gatcctgaga cgcttcatcg ccttcgccga
     1921 aagccaaagc accgatcaca tcagcaccga tctgttcgtg aagtggcaag gcgtgttcgg
     1981 ccaggcaagc cgacaaacct gggctgcccg actctgcatt gtgcggctgt tcgcccaatg
     2041 gctgcacaac cttgacccgg cccatgagat tccaccacag ggtctgattc caaaccggtc
     2101 ccgacgctcc cggccgcaca tatatagcga agaggagatt gcgaagatcg tcgcggctgc
     2161 cgccgaactc ccttcggttt atggcatgcg cggccttacc tatgccacgc tgttcggcct
     2221 catagcgatg acggggctgc ggatcagcga ggccctggcg ctcaacgtcg acgacgtcga
     2281 tctcgatctc ggcgtgctga ccatccggcg cggcaagggt ggcaaggcgc ggttcgtac
//
LOCUS       DATGEM010000318         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1637939, whole genome shotgun sequence.
ACCESSION   DATGEM010000318 DATGEM010000000
VERSION     DATGEM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637939"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VKQ27_10215"
     CDS             <1..784
                     /locus_tag="VKQ27_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89349.1"
                     /translation="ADAHTKLVVGHHPAFSVNGFAGAYERDIGPEHASSFWNVLVSHN
                     VLAYLCSHILAFDVQVHRGVLQLCTAGAGTLHRMPEKFEYLHCTQLAIDGAGLRCQVL
                     DTTGAVREAFHWPPAADTGLSFRITGRTTAEAGPAQTLASASDPGILSPLWIGLCGPH
                     QVLCVVIGPEPGRSPHTWHGPTIQPDQPFEIRLRLDPTMGPGGLLCQLNDETSWSSLH
                     GASPWGVERLRLPSEWSMGHGQYGADDRPFLGNGLSVSIIGN"
     gene            799..1824
                     /locus_tag="VKQ27_10220"
     CDS             799..1824
                     /locus_tag="VKQ27_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLY89350.1"
                     /translation="MRARDLGLGCGSLPPGEDNTIADVPGITVGHATLIKADIRTGVT
                     AVLPHEGDLFRDKVTAASVVLNGFGKSTGLVQVHELGTLETPILLTNTFAVGTCANAL
                     IRRAIAANQEIGRRTATVNPVVLECNDGYLNDIQAMAVTETHANAAIDAASKTFTVGS
                     VGAGTGMSCFGLKGGIGSASRQFHLDGSSCHLGVLVLANFGRPGDLRLPDGRRVEATA
                     PQAAETGSCIILIATDVPLDHRQLTRVARRAGVGLAWCGSFWGNGSGDIALAFSTANR
                     VPHTADRDLIQHRILAETRIDLLFEAVGEATQEAVLDALTSSETMLGRSGHQRTGLRD
                     LMSPPRN"
     gene            complement(1855..2238)
                     /locus_tag="VKQ27_10225"
     CDS             complement(1855..2238)
                     /locus_tag="VKQ27_10225"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HLY89351.1"
                     /translation="MNPERLLRAYRRTEYSAAGCIVLIGRRSPDRLFQVLGAYSAVFI
                     TAWNPMSRRMPDGWNRQAQRRLRERLRRFGSVPADGTLGRWHEAHLLVSVADMRPMVR
                     LARIFRQRAVVVIKRDHPAKLVVVA"
     gene            complement(2235..3383)
                     /gene="rodA"
                     /locus_tag="VKQ27_10230"
     CDS             complement(2235..3383)
                     /gene="rodA"
                     /locus_tag="VKQ27_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HLY89352.1"
                     /translation="MREGRLWLQTSFGLGNKVLQINWLYVLLLCCLAGVGYGALYSAA
                     GGSTEPYAARHVMRFAAGLVMLVGIAMVDIRFIKRLAWPSYFISLVLLVVVMRYGHVG
                     KGAQRWIEFGGLQLQPSELMKISLVLALASWFHRASWERIGNPLFLIPPLLAVLVPVA
                     LIVKEPNLGTAVITAILGGVMFFAAGVRWWKFLLVALPIPFIAKIAYGHLHDYQRARI
                     DTFLNPENDPLGAGYNIIQSKIALGSGGMWGQGYLQGSQGHLDFLPEKQTDFIFTMIG
                     EEFGFVGGVAVMALLCLIVLCGMLVALRCRHQFGRLVALGIATNFFLYVFVNIAMVMG
                     AIPVGGVPLPLVSHGGSAMFTVMIGFGVLMSVHVHRDVEFTEPRNALA"
     gene            complement(3442..5313)
                     /gene="mrdA"
                     /locus_tag="VKQ27_10235"
     CDS             complement(3442..5313)
                     /gene="mrdA"
                     /locus_tag="VKQ27_10235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLY89353.1"
                     /translation="MRRDTRRTGVFTRRALLLMGGQVAVLGGLAARLYQVQVLEGARY
                     ATLARENRVSARLIAPPRGRMLDRFGTVVAGNKLNWRALLVAEQTTDLTATLDSFSRV
                     VPLSDYERARIEREVRRHRRFIPVMVREFLNWDEMAAIEVNAPDLPGIIVDVGTTRMY
                     PFGDHLAHVIGYVAPPSEADVEADAALSLPGIRIGRAGLEKQYDQDLRGRAGAVQVEV
                     NAYGRVIRELDRQEGAEGEELGLTIDAQLQQQVIARLGDECASAVVLDCTNGEVLAMA
                     TNPSFDPSLFDAGVSQAQWKAWSQDKRTPLLNRAIAGVYAPGSTFKMNVAMAALEAKA
                     ITTADTFECPGFFDLGDARFHCWRKGGHGTLNLRGGLKNSCDVFFYQVAKKVGIDPIA
                     AMGHNFGLGIELAIDLPGVRAGLIPTREWRRAQGHPWNLGDTVVAGIGQGYIQVSPLE
                     LATYVARVATGREVVPHLTRKLAGELQPGSDPADWPALGLTERFLHAVREGMWAVVNE
                     AGGTAPQAKLPDQRWQLAGKTGSSQVRRVSRAQREETNFDSSKLPWDQRPHALFVAYA
                     PYDAPRYAVSVVVEHGNAGAAAAAPIARDIMMDTLQRDPGGRDTPPGDKVAMNQVTR"
     gene            complement(5313..5867)
                     /locus_tag="VKQ27_10240"
     CDS             complement(5313..5867)
                     /locus_tag="VKQ27_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HLY89354.1"
                     /translation="MARADQTPGIRPRATFARQLDIAARHGFPATCTILLMLLTQIPF
                     GFADQAALLPAVTLACVYFWSLFRPSGMPPPVVFVIGLLLDLLGYLPLGVGALALLLV
                     HGLVLRWRRVLSQQGFLPVWLAFAGFAAGASALAWILTDVLTFRLLPPWPAIFEAALT
                     AALYPALAILFVRAHRGVADPDRV"
     gene            complement(5867..6730)
                     /gene="mreC"
                     /locus_tag="VKQ27_10245"
     CDS             complement(5867..6730)
                     /gene="mreC"
                     /locus_tag="VKQ27_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLY89355.1"
                     /translation="MLRLSIQARQALAKLTLPVLIAACFGLMLLGKADAVVGERLRMA
                     LADALAPIYLALAEPLAGLRDMVSGTTDLWDVRAQNEQLRVENEQLRRWQSIALALDA
                     ENERLKANLHWIPDSAPSYVTARVVADAGGVYAKAVLLSVGPNHSIRKGEIAVDERGL
                     VGRVTEVGARTARVLLLTDLNSRIPVILEHSHAHAILLGTNGPRPRLMFWPEGSAPRE
                     GEQVITSAEAGAFPADLPVGTVHYTNSNVPEVVPTALLDRLEIVRIFDYGLTGVDDPE
                     AAARAMQGRAY"
BASE COUNT         1360 a         2399 c         2009 g         1099 t
ORIGIN      
        1 tgccgacgcg cacaccaaac tcgttgtcgg ccaccacccg gcattttccg tgaacggctt
       61 cgcaggcgct tatgagcggg acatcggacc ggaacacgcc agctccttct ggaacgtgct
      121 cgtctcgcac aacgtactcg cttatctgtg cagccacatc cttgcattcg acgtccaggt
      181 gcaccgcggc gtgctgcagc tctgcacagc gggtgcaggc acgctgcatc gcatgccgga
      241 gaagtttgaa tacctgcact gcacacagct cgccatcgac ggtgccggtc ttcgctgcca
      301 ggtgctcgat acgaccggcg ccgtgcgtga ggctttccac tggccgcctg ctgccgacac
      361 aggtctgtcc ttccgcatca ctggacgcac caccgcggag gccggtcccg cacaaactct
      421 cgcctcggcc tccgatccag gcatcctttc gccgttgtgg attggcttgt gcggaccgca
      481 tcaggtccta tgcgtcgtca tcggtccgga accgggccgc agtcctcaca cgtggcacgg
      541 ccccaccatc caacccgatc agccgtttga gatccgcctg cggctcgacc caacgatggg
      601 acccggagga ttgctgtgcc aactgaacga cgaaacatcg tggtcttcgc tgcacggcgc
      661 atcaccgtgg ggagtggaac ggctgcgcct cccatccgaa tggagcatgg gccacggtca
      721 gtatggtgca gatgatcgac cgttcctcgg gaatggtctg tctgtctcaa tcatcggcaa
      781 ctaaagaaag cgacgcccat gcgcgcacgt gatctcggcc tgggctgcgg ctcgctgccg
      841 cccggcgaag ataacacaat cgctgacgtt cccggcatca ccgtcggcca tgcaaccttg
      901 ataaaggccg acatccgcac cggcgtcacc gccgttctgc cgcacgaagg cgacctgttc
      961 cgggacaagg tgacggccgc gagcgtggtg ctgaacggct tcggcaagag cacgggcctc
     1021 gtgcaggtgc acgaactcgg cacactcgaa acaccgatcc tcctcaccaa cacctttgcc
     1081 gtcggtacct gcgccaacgc actgatccgc cgcgcgatcg ccgccaacca agaaatcggt
     1141 cggcggaccg ccacagtgaa tccggtcgtc ctggagtgca acgacggcta cctgaatgac
     1201 atccaggcga tggccgtcac cgagactcac gccaacgcag caatcgatgc cgcttcgaag
     1261 acctttacag tcggctctgt gggggccggc accggcatga gctgttttgg cctgaagggc
     1321 ggcatcggca gtgcgtcgcg tcagttccac ctcgacggct cgtcctgtca tctcggcgtg
     1381 cttgtcctcg cgaatttcgg ccgccccggc gatctacgct tgcccgatgg gcgccgtgtc
     1441 gaggccacgg caccacaggc cgcggaaacc ggttcctgca tcatacttat cgccaccgac
     1501 gtgccgctcg accatcggca gctcacacgt gtggcaaggc gcgctggtgt cggcctggca
     1561 tggtgcggct cattctgggg caatggcagc ggtgatattg ccctggcatt cagcaccgcc
     1621 aaccgcgtcc cgcacacggc ggatcgcgac cttatccagc atcgcatact cgctgaaacg
     1681 cgcatcgatc tgctcttcga ggccgtgggc gaagccactc aggaagctgt tctcgatgcg
     1741 ctgacgagct ccgagaccat gctgggaagg agtggccatc agcgcacagg attgcgcgac
     1801 ttgatgtcac ctccccgcaa ttgactgcag agctgaggcg ccgccatcgc cgcgctacgc
     1861 caccaccacc agcttcgccg ggtggtcccg cttaatcacg acaaccgctc gctgacggaa
     1921 gatcctggcc aaacgcacca tcggacgcat gtcggcaaca gaaaccagca gatgcgcttc
     1981 atgccagcgt ccaagcgtcc cgtccgcggg caccgatcca aaacgccgca accgctcccg
     2041 cagccggcgt tgtgcctgcc ggttccagcc atccggcatc cgccgcgaca tcgggttcca
     2101 ggcggtgatg aacacagccg aatacgcacc cagtacctga aacaggcgat cgggagagcg
     2161 ccgcccaatc agcacaatgc atcccgcggc cgagtattcc gttcggcgat aggcgcgcaa
     2221 caaccgctcg gggttcacgc cagtgcgttg cgtggctcgg tgaactccac atcgcgatgc
     2281 acgtgaactg acatcagcac accgaagcca atcatcacgg tgaacattgc tgacccgcca
     2341 tgcgagacca gcggcagggg tacgcccccc accggaatgg cgcccatcac cattgcaata
     2401 ttgacgaaca cataaaggaa gaagttcgtg gcaattccca gcgcaaccag ccgtccgaac
     2461 tggtgacggc agcgcagcgc caccagcatg ccgcagagca cgatcaggca gagcagcgcc
     2521 atcaccgcca cgccgccaac aaagccgaac tcctcaccaa tcatggtgaa tatgaagtcg
     2581 gtctgctttt ccggcaggaa atccaggtgg ccttgcgacc cttgcaggta gccctgcccc
     2641 cacatcccac ccgaccccag tgcaatcttc gactggatga tgttgtagcc ggcaccgagc
     2701 ggatcgtttt ccggattcag aaaggtatcg atgcgagccc gttggtaatc atgcagatga
     2761 ccataggcga tcttggcaat gaacgggatc ggcagtgcga ccagcaggaa cttccaccag
     2821 cgcactccgg cggcgaagaa catcacgcca ccgaggatcg ccgtgatgac cgcggtgcca
     2881 aggttcggct ccttcacgat cagtgccacg ggcaccagca ccgcaagcag cggcgggatc
     2941 aggaacagcg gattgccaat gcgctcccac gaggcgcggt ggaaccacga cgccaacgct
     3001 agcaccaggg agatcttcat aagctccgac ggctgcaact gcaggccgcc aaactcgatc
     3061 cagcgctgcg cgcccttgcc cacgtggcca taccgcatga ccaccacgag caacacgagc
     3121 gaaatgaaat agctaggcca cgccagccgt ttgatgaatc ggatgtccac catcgcgatg
     3181 ccgacaagca tcaccagccc ggcggcgaag cgcatcacgt gtcgcgccgc atagggttcc
     3241 gtcgaaccac ccgcggcgct gtacagcgcg ccataaccca cacccgccag gcagcacagc
     3301 agcaacacgt acagccagtt gatctgcagc accttgttgc caagcccaaa cgaggtctgc
     3361 agccagagcc gcccttccct catgcgtggt ttcgggcgca agcccgactt cgagccgtca
     3421 gggccggact tgatccggcc ctcatcgcgt cacctgattc atcgcgacct tgtcgccagg
     3481 tggcgtatcc cgcccgcctg gatcgcgctg cagtgtgtcc atcatgatgt cgcgtgcgat
     3541 aggcgcggcc gcggcggcgc ccgcattgcc gtgctccacc acaacggaaa ccgcatagcg
     3601 cggcgcgtca tacggtgcat aagcgacaaa cagggcatgt ggccgctgat cccagggcag
     3661 cttcgaactg tcgaagttgg tttcctcgcg ctgcgcacgc gagacgcgac gcacctgcga
     3721 cgaaccggtc ttgcccgcca gctgccaacg ctggtcgggt agcttcgcct ggggcgcagt
     3781 accgccagcc tcgttgacca ctgcccacat cccttcgcgc accgcgtgca gaaagcgctc
     3841 ggtcaggccc agcgctggcc agtcggcggg atccgacccc ggctgcaatt ctccagccag
     3901 cttgcgcgtc agatgtggca ccacctcacg accggtagcc acacgcgcga cataggtggc
     3961 gagttccagt ggcgagactt ggatataccc ctggccaatg cctgcaacga ccgtatcgcc
     4021 caggttccac ggatggccct gagccctccg ccactcgcgc gtcggaatca gcccggctcg
     4081 cacacctggc agatcgatcg ccagctcaat ccccaggccg aagttgtggc ccatggctgc
     4141 gatgggatcg atgcccacct ttttcgccac ctgatagaag aacacatcac agctgttctt
     4201 cagccccccg cgcaggttca gcgtgccgtg cccaccctta cgccagcaat ggaaccgcgc
     4261 atcgccgaga tcgaaaaagc caggacactc gaacgtatcg gccgtcgtga tcgccttcgc
     4321 ctccaaagcg gccatcgcga cattcatctt gaatgtcgaa ccgggtgcat atacgccggc
     4381 gatcgcccgg ttcagcaaag gcgtgcgctt gtcctgcgac caggccttcc actgcgcctg
     4441 cgacacacca gcatcgaaca gcgacggatc gaacgacggg ttggtggcca tcgccagcac
     4501 ctcgccattg gtgcagtcga gcaccacggc gctggcacac tcatctccga ggcgggcaat
     4561 aacctgctgc tgcaactgcg catcgatcgt caggccaagt tcctcgccct cggcaccctc
     4621 ttgccgatcc agttcgcgga tcacccgccc ataggcgttc acctcgacct gcaccgcgcc
     4681 tgcacggcca cgcaaatcct ggtcatactg cttctccagg ccggcgcggc cgatacggat
     4741 gcctggcagc gacagcgccg cgtcggcttc gacatccgcc tccgacggcg gtgccacgta
     4801 gccgatcaca tgggcaaggt gatcgccgaa cggatacatc cgtgttgtcc cgacatcgac
     4861 gatgatgccg ggcagatcgg gcgcattcac ctcgatcgcc gccatctcgt cccagttcag
     4921 gaactcccgc accatcacgg ggatgaaccg gcgatgccgg cgcacctctc gctcgatccg
     4981 cgcccgctcg tagtcggaaa gcgggacgac acgcgagaaa ctatccagcg tggcggtgag
     5041 atcggtggtc tgctcggcaa ccagcagcgc acgccagttg agcttgttgc ctgcgaccac
     5101 cgtgccgaac cgatccagca tccggccgcg cggaggcgcg atcagccgtg cactcacgcg
     5161 attctcgcgt gccagcgtgg catagcgggc accttccagc acctgcacct gatacaggcg
     5221 cgccgccagg cccccgagca ccgcgacctg cccgcccatc agcagcaggg cgcgtcgcgt
     5281 gaaaaccccg gtccggcgag tatcgcgacg catcacacgc ggtccggatc agcgacgccg
     5341 cgatgagccc gcacgaacag gattgccagc gccggatata gcgctgccgt cagtgcagcc
     5401 tcgaatatcg ccggccaggg gggcagcaag cggaaggtca gaacgtccgt caggatccag
     5461 gccaacgccg aggcgcccgc agcaaatccg gcaaacgcca gccaaaccgg cagaaagccc
     5521 tgctgactca gcacgcgccg ccagcgcagc accagcccat gcaccagcag caaggccagc
     5581 gcaccgacac ccagcggcag atagccaagc agatcgagaa gcaggccgat cacgaacacc
     5641 accggcggcg gcatgccgct cggccggaac agcgaccaga aatagacgca ggccagcgtc
     5701 accgccggca atagcgccgc ctgatcggcg aagccgaacg ggatctgggt cagcaacatc
     5761 agaaggatgg tgcaggtcgc cgggaacccg tgccgggcag caatgtcgag ctggcgcgcg
     5821 aaagtggccc gcggccggat cccgggtgtc tgatcggcac gcgccatcag taagcacgtc
     5881 cctgcatggc ccgcgccgca gcttcgggat cgtcaactcc ggtcaggcca tagtcgaaga
     5941 tccgcacgat ctccaggcgg tccagcaagg ccgtgggcac cacctccggc acgttggaat
     6001 tggtatagtg gacggtgccg accggcagat ccgcaggaaa cgcgcctgct tccgcgctcg
     6061 ttatcacctg ctcaccctcc cgcggcgccg aaccttccgg ccagaacatc agccttggcc
     6121 gaggtccgtt cgtgcccagc aggatggcat gcgcgtgtga atgctccagg attaccggga
     6181 tgcgactgtt gaggtcggtc agcaacagaa cacgcgccgt cctcgccccc acttcggtga
     6241 cccgtcccac caggccacgt tcgtccactg cgatctcgcc cttgcggatg ctgtggttcg
     6301 ggcccaccga cagcaggaca gccttggcgt agacaccgcc ggcatccgcc acgaccctgg
     6361 cggtgacgta cgacggcgcg gaatcaggga tccaatgcag gttggccttc aaccgctcgt
     6421 tctcggcgtc cagcgcgagc gcgatcgact gccacctgcg caactgttcg ttctcgacgc
     6481 gaagctgctc gttctgggcg cggacgtccc acaggtcggt tgtgccggag accatatcac
     6541 gcaatccagc cagcggttca gccagcgcga gatagattgg cgcgagtgca tccgccagcg
     6601 ccatccggag acgctctcct actaccgcat cggccttgcc gagcagcatc aggccgaagc
     6661 atgcagcgat cagcaccggc agggtaagtt tggccagggc ctgccgggcc tggatggaaa
     6721 gacggagcat ggagaggcgg tcctagtgga cagcccctct atacgcgaac cgccgcgagg
     6781 cacaaagccc cttatttttc agcggcttgg accgttacga accgccctgc ccgcggtcca
     6841 gcagtttgcg gtcggactgc accgtat
//
LOCUS       DATGEM010000319         5157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_594078, whole genome shotgun sequence.
ACCESSION   DATGEM010000319 DATGEM010000000
VERSION     DATGEM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594078"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VKQ27_10250"
     CDS             complement(<1..560)
                     /locus_tag="VKQ27_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLY89356.1"
                     /translation="MASNIVGRVTQVQGAVVDVQFDGELPFIQNALHTKLSEHTLVLE
                     VAQELGERTVRCIAMDSTDGLVRGQEVVDTGGPITVPVGPETLGRILNVIGEPIDEQG
                     PINTKMRYPIHRDAPSFEEQATSAEVLVTGIKVVDLLAPYLKGGKVGLFGGAGVGKTV
                     IIQELINNIAKAHGGVSVFAGVGERTR"
     gene            complement(577..1458)
                     /locus_tag="VKQ27_10255"
     CDS             complement(577..1458)
                     /locus_tag="VKQ27_10255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HLY89357.1"
                     /translation="MPSLKDLRTRINSVKSTQKITSAMKMVAASKLRRAQAQAEAARP
                     YAERMERMLRALAASVSDMPNAPALLAGTGKQQNYLLVPVTADRGLAGAFNASIGRAT
                     RTLARRLEAEGKTVKILPVGRKGRDYLRRELASRITSDISFAGKRRLEFSDAEDIGVR
                     ITTMLAAGEVDVCILIYNRFRSVISQIVTEQQLIPAPPAPEGEAVDLGGASYEFEPDE
                     ETILTRLLPQALAIAIFRALLESAAGEHGARMTAMDNATRNAGDMIKRLTLNYNRARQ
                     ANITKELIEIISGAEAV"
     gene            complement(1558..3090)
                     /gene="atpA"
                     /locus_tag="VKQ27_10260"
     CDS             complement(1558..3090)
                     /gene="atpA"
                     /locus_tag="VKQ27_10260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLY89358.1"
                     /translation="MEIRPAEISEILKKQIAAFDTEANVAETGQVLSVGDGIARVFGL
                     QNVMAGEMVEFPGAGLRGMALNLETDNVGVVIFGDDKQIREGDTVSRTGSIVDVPVGD
                     ALLGRVVDGLGNPIDNKGPIAAAERRRVEVKAPGIIPRKSVHEPMQTGLKAIDALIPI
                     GRGQRELIIGDRQTGKTAVIIDTIINQKQINAGSDESKKLYCIYVAIGQKRSTVAQLV
                     QTLEEYGAMQYSIVVAATASDPAPMQFLAPYTGCTMGEYFRDNKRHAVIFYDDLSKQA
                     VAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMSEEYGLGSLTALPVIETQAGD
                     VSAYIPTNVISITDGQIFLETELFFRGIRPAVNVGISVSRVGSAAQVRAMKQVAGRIK
                     LELAQYREMAAFAQFASDLDASTQQLLARGSRLTELLKQPQFKPVPVEEQVVAIFAGV
                     RGYLDKIDLNQVGRFEAGVLSELKSREPAILDSIRTDLEIKPDTEKKLVAFMDNFAKS
                     FA"
     gene            complement(3090..3659)
                     /locus_tag="VKQ27_10265"
     CDS             complement(3090..3659)
                     /locus_tag="VKQ27_10265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HLY89359.1"
                     /translation="MVSGATTIASGGGLADRYAAALYAHADDQHALDQVVSEMETLGR
                     IIEASADFRRLLDSPLIDVNQATRAALAVLEQEGFSKPVRDFVGVISSNRRLRNLPDI
                     VEAFAHLVAERRGIVTAHVATAHPLTDVQRQQLRARLIEAGYGNVNIHERVEPDLLGG
                     LVVRIGARLYDTSLKSRLQRLQYAMKGAA"
     gene            3831..4103
                     /locus_tag="VKQ27_10270"
     CDS             3831..4103
                     /locus_tag="VKQ27_10270"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLY89360.1"
                     /translation="MDDFAAVPSVGAYEAKTHLSELLDRVERGEQIVITRHGRPVARL
                     VPEGREDVEEALTALDRITARRKALAARGVRLTQAEMRALREEGRR"
     gene            4114..4524
                     /locus_tag="VKQ27_10275"
     CDS             4114..4524
                     /locus_tag="VKQ27_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY89361.1"
                     /translation="MVTCVLDASITVAALIEEERSDEARGVFRAIIDGSAVVPGLWAL
                     EVGNILLILERRRVLSAIARREHLDDLSRLPIVVDHETTARAWQETMTLAERHVLTLY
                     DAAYLELSLRRQLPLATFDEALRKAAAAVNVHVL"
     gene            4678..>5157
                     /locus_tag="VKQ27_10280"
     CDS             4678..>5157
                     /locus_tag="VKQ27_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89362.1"
                     /translation="MPSRIARRTALRLAGAAAGSGAITGFPTIWAQNIKNVTLRQFGT
                     GVSNLNAIADKVKQDLGFRLEMTALDTDAVVQKAVTQPKSYDVADIEYFSCKKVFPTG
                     VLQPMDTKKIANYEKIVPIFITGKLTPASIIAQGTAPHTVGFVEAKNSTKFAPHPTEW
                     "
BASE COUNT          844 a         1610 c         1736 g          967 t
ORIGIN      
        1 cgcgtgcgct cgcccacgcc ggcgaataca gacacgccac cgtgcgcctt ggcgatgttg
       61 ttgatcagct cctggatgat cacggtcttg cccacgccag cgccgccgaa caggccgacc
      121 ttgccgccct tgaggtaggg cgccaggagg tccaccacct tgatgccggt gaccaggact
      181 tctgccgatg tcgcctgctc ttcgaaggac ggggcatcgc ggtgaatcgg atagcgcatc
      241 ttcgtgttga tcgggccctg ctcgtcgatc ggctcgccga tcacgttcag gatgcggcca
      301 agcgtctcgg ggccgacggg cacggtgatc gggccgccgg tgtccaccac ttcctgcccg
      361 cgtaccaggc catcggtgct gtccatcgcg atgcagcgga ccgtgcgctc gccgagttcc
      421 tgggcgactt cgagcaccag tgtgtgctcg gagagtttgg tgtgcagcgc gttctggatg
      481 aacggcagct cgccgtcgaa ctgcacgtcc acgacggcgc cctgcacctg ggtcacgcgg
      541 ccgacgatgt tgcttgccat atctcttctg cccctgtcag acggcttcag cgccggaaat
      601 gatttcgatg agttccttgg tgatattggc ctggcgcgcg cggttgtagt tgagcgtcag
      661 gcgcttgatc atgtcgccgg cattgcgcgt tgcgttgtcc atggcggtca tgcgcgcgcc
      721 atgctcgccg gccgcgcttt ccagcagcgc gcggaagatc gcgatggcaa gtgcctgcgg
      781 cagaaggcgg gtcaggatgg tttcctcgtc cggctcgaat tcgtagctgg cgccaccgag
      841 atcgaccgct tcgccttccg gtgcaggcgg cgcaggaatc agctgctgct cggtgacgat
      901 ctgggagatc accgaacgga agcggttgta gatcaggata cagacgtcaa cctcgcccgc
      961 ggccagcatc gtggtgatgc ggacgccgat atcctccgcg tcgctgaatt ccaggcggcg
     1021 cttgccggcg aaggagatgt cgctggtgat gcggctggct agttcgcggc gcagatagtc
     1081 gcggcccttg cggccgacag gcaggatctt gacggtcttg ccttcggcct ccaggcggcg
     1141 tgcgagcgtg cgggtcgcgc ggccgatact ggcgttgaac gcgccagcga ggccgcgatc
     1201 ggcggtgacc gggaccagca ggtagttctg ctgcttgccg gtgccggcga gcagggcagg
     1261 ggcgttcggc atgtcggaca cagaggcggc gagggcacgc agcatgcgtt ccatgcgctc
     1321 ggcatagggg cgcgcggctt ccgcctgcgc ctgggcgcgg cgcagcttcg acgccgccac
     1381 catcttcatg gcagaggtta ttttctgcgt cgacttcacg ctgttgatgc gtgtgcggag
     1441 gtctttcagg ctaggcaagg ttcacctgcg gtcactgtca ttgcgagcaa aacgaagcaa
     1501 tcccgtccga aattgctgga tcgctgcgct actcgccatg acatgggtgg catggggtta
     1561 ggcaaagctc ttggcgaagt tgtccatgaa ggcaaccagc ttcttttccg tatctggctt
     1621 gatctccagg tcggtgcgga tcgagtccag aatcgctggc tcgcgcgact tcagttccga
     1681 cagcaccccg gcctcgaagc ggccaacctg gttgaggtcg atcttgtcga gatagccgcg
     1741 cactccggcg aagatcgcaa caacctgctc ctcaaccggc acaggcttga actgcggctg
     1801 cttcagcaac tcagtcagcc gcgaaccgcg cgccagcaac tgctgcgtgg aggcatccag
     1861 gtccgaggcg aactgcgcaa acgctgccat ctcgcgatac tgcgccagct ccagcttgat
     1921 gcgcccggcg acctgcttca tcgcgcgtac ctgtgcggca gaacccacgc gcgacaccga
     1981 gataccgacg ttcaccgccg gacggatgcc gcggaagaac agctcggtct ccaggaagat
     2041 ctggccgtcc gtgatggaga tcacgttggt cgggatgtag gcggacacgt cgccggcctg
     2101 cgtctcgatc accggcaggg ccgtgagcga cccaagcccg tactcctcgc tcatcttcgc
     2161 cgcgcgctcc agcaggcgcg agtgcaggta gaacacgtcg cccggatacg cctcgcgtcc
     2221 tggcgggcgg cggagcagca gcgacatctg gcgatacgcg acagcctgct tcgacaggtc
     2281 gtcgtagaag atcaccgcgt ggcgcttatt gtcgcggaag tactcgccca tcgtgcagcc
     2341 ggtgtatggc gccaggaact gcatcggcgc cggatcggac gccgtggcgg cgaccacgat
     2401 ggagtattgc atcgcgccgt attcttccag cgtctgcacc aactgcgcca ccgtggagcg
     2461 cttttggccg atcgcgacgt agatgcagta gagcttcttg ctctcgtcgg agccggcatt
     2521 gatctgcttc tggttgatga tcgtgtcgat gatcaccgcc gtcttgcccg tctggcggtc
     2581 gccgatgatc agctcgcgct gaccgcggcc gatcgggatc agcgcgtcga tcgccttgag
     2641 accggtctgc atcggctcgt gcaccgactt gcggggaatg atgccgggcg ccttcacctc
     2701 gacgcggcgg cgctcggcgg cagcgatcgg gcccttgttg tcgatcggat taccgaggcc
     2761 atccaccact cggccgagca gcgcgtcgcc caccggcacg tccacgatgg agcctgtgcg
     2821 gctgacggtg tcaccttcgc ggatctgctt gtcgtcgccg aagatcacca cgccgacgtt
     2881 gtcggtctcc aggttcagcg ccatgccgcg caggccggcg cccgggaact cgaccatctc
     2941 accggccatg acgttctgca ggccgaacac gcgggcgatg ccgtcaccga cggacagcac
     3001 ctggccggtc tcggcgacgt tggcttccgt gtcgaacgcg gcgatctgct tcttgaggat
     3061 ctccgagatc tcggcggggc ggatctccat caggcggctc ccttcatggc gtactgcagg
     3121 cgctgcaggc gtgacttaag cgaggtgtcg taaaggcgcg ccccgatccg gacgacaagg
     3181 ccgccgagca gatcgggttc cacccgttcg tggatgttga cgttgccgta gccggcctcg
     3241 atcaggcggg cgcgaagctg ctggcgctgc acgtcggtca gcgggtgcgc ggtggcaacg
     3301 tgggcggtga cgatgccgcg gcgctcggca acaagatggg caaaggcttc gacgatgtcc
     3361 ggcaggttgc gcagccggcg gttggacgaa atcaccccga cgaagtcgcg cacgggcttg
     3421 gagaagcctt cctgctcgag gacggcgagc gcggccctgg tggcctggtt cacgtcgatc
     3481 aggggcgaat ccagcaggcg gcggaaatcg gcgctggcct cgattatgcg gccgagggtc
     3541 tccatttcgg agaccacctg gtcgagcgca tgctgatcgt cggcatgcgc gtagagagcg
     3601 gcggcatagc ggtcggcgag gccgccgccc gatgcgatcg tcgtagcgcc agacaccagg
     3661 tggcgttccc tatgttggcc gcgccagagc gcgggcgtga aacgggggcc ggccgaggtt
     3721 cggctggcgg cgcggggcct gtagcatggg gtctggtgcc tcgcaacata gtgcaaccaa
     3781 ttctcgtgcg tcgctttgca ctggtcggac cactggtcta aatactgctc atggatgatt
     3841 tcgctgctgt gccgtccgtg ggcgcgtatg aggccaagac ccacctgtcg gagctcctcg
     3901 accgcgtgga gcgcggcgag cagatcgtca tcacccgtca tgggcgtccc gtggcgcgcc
     3961 tggttcctga aggacgagag gatgtcgagg aggcactgac ggcgcttgat cgtattacag
     4021 cccggcgcaa ggcgctcgcc gcgcggggcg ttcgcctgac gcaggccgag atgcgggcgt
     4081 tgcgcgaaga aggtcgccgc tgatcggcac ccagtggtca cgtgcgttct cgacgcgtcg
     4141 atcacggttg cggcgctgat cgaagaggag cgttccgatg aggcgcgtgg cgtgtttcgc
     4201 gcgattattg acgggagcgc ggtcgttcct gggctgtggg cgcttgaggt cggcaacatc
     4261 ctgctcatcc tcgagcgtcg gcgcgtgctg tctgccatcg cgcgacggga gcatttggat
     4321 gatctctcac gtttgccgat cgtcgttgac catgaaacga cggcgcgcgc ttggcaggag
     4381 accatgacgc tggccgaacg tcatgtgctc acgctgtatg atgccgctta tctcgagttg
     4441 agcctgcgcc gccagttgcc gcttgcgaca ttcgacgaag cactacggaa ggcagcggct
     4501 gccgtgaacg tccacgtgct ttgataccgt gctgcctatt gttgaggcgc gatcgccgcc
     4561 ctcctgacgc caaataggtc agtcgtgcca caggccggtg ctgcgctgcg ccgtcgtccg
     4621 ttgtggtacg tcgattgcct gctgcatcgc ggattggcca gcaaaagggg aacgtttatg
     4681 ccaagcagga tcgctcgccg caccgcattg cgcctcgccg gtgccgcggc gggatcgggc
     4741 gccatcaccg gctttccgac gatctgggcg cagaacatca agaacgtgac cctgcggcag
     4801 ttcggcaccg gcgtgtccaa cctcaatgcc atcgccgaca aggtgaagca ggacctcggc
     4861 ttcaggctgg agatgaccgc gctcgatacc gatgcggtgg tgcagaaggc ggtgacgcag
     4921 ccgaaatcct atgacgtggc ggatatcgaa tacttctcct gcaagaaggt gttccccacc
     4981 ggcgtgctcc agccgatgga taccaagaag atcgcgaact acgagaagat cgtgccgatc
     5041 ttcatcaccg ggaagctgac gcccgcttcc atcatcgcgc aggggacggc gccgcacacg
     5101 gtcgggtttg tcgaggcgaa gaactcgacg aaattcgcgc cgcatccgac cgagtgg
//
LOCUS       DATGEM010000320         6709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_472613, whole genome shotgun sequence.
ACCESSION   DATGEM010000320 DATGEM010000000
VERSION     DATGEM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472613"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="gspM"
                     /locus_tag="VKQ27_10285"
     CDS             complement(<1..519)
                     /gene="gspM"
                     /locus_tag="VKQ27_10285"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HLY89363.1"
                     /translation="MSLPEGRRGQFLATALTLAVLAAAWLGVGKPLLDWYQSGVDTLV
                     QRQMLLRHMEALAQTLPTLEQTPAAARRESTPLLSGATDALAAAAMQNAVQAMATRAG
                     VELASMETLPAEARGGYRRIGLRVSLTAPWPVLVELLRVASQGEPRMLVDDVQLRAMP
                     MPGNAPAAMPVGA"
     gene            complement(527..1501)
                     /locus_tag="VKQ27_10290"
     CDS             complement(527..1501)
                     /locus_tag="VKQ27_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLY89364.1"
                     /translation="MHALLPWGLLRGGEGGDALMIGARGPHVVLTLRRGGRENPLGQF
                     GAAGDGLAVAVRALRRLPRRVVLQLGPGTLLERPVELPLAAERDIDRVIGFEMDRLTP
                     FAADEVVWQAAVMRRDHAQKRLVLRLSLAPRRAIQPWLDMLARAGLAPGWLELPAADG
                     SPRRIALADHVAPRPSRVFGIGASLVAALAVAVIATPFVMQYFARSTTERTIVALQPQ
                     VKRVEALRRQQTDASGDVLAAERGRIGNVIQVLATLTDILPDDAWLTEFTLHQGKLNI
                     AGQSPNAARLIPALAAEPTLRNPAFAAPVTRAPDGRTDLFVIHADLAP"
     gene            complement(1655..2887)
                     /locus_tag="VKQ27_10295"
     CDS             complement(1655..2887)
                     /locus_tag="VKQ27_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLY89365.1"
                     /translation="MAQFRYTAIGAGGEMLRGVLEAGSEAEVVARLQRQGSLPMRAEP
                     VGDGGFLGGLLSADFLPRQKLSAQQIADVTRELATMLGAGQDLDRALRYALETAPNAR
                     VRRVLEGIRNAVRDGSPLSQALAREPASFSRLYVGMVRAGEASGRLAEALARLAELLE
                     RQRRVAATVTSALIYPCLLAVAAVGAVTLLLTEVLPQFVPLFEQSGARLPGSTQFLID
                     AGAFLSNWGIGLLCAGLGLVLACGALLRRPGPRLAADRLILRLPVIGHLQRELMAARL
                     TRALGTLLVNGVPLIGALAIAREALGNRVAMLALDRAGQSARGGGSLAQPLGESGVFP
                     ARTIHLLRLGEENAQLGTMALHAAEIHEERSRIAVQRLVALMVPAITIVMGAAVAGIV
                     ASLLTAMLSLNDLAAAGG"
     gene            complement(2966..3826)
                     /locus_tag="VKQ27_10300"
     CDS             complement(2966..3826)
                     /locus_tag="VKQ27_10300"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89366.1"
                     /translation="MTMGSPTTDAREPSNRVMAPGGQCLPGERGFALLIVLFVLGFLA
                     LLETHFVATGRTWMAMARNSRAAAQVEAATAGAIQGEIFYLLSGAGSAPRKVRIGATL
                     VDLAVANESDLLNPNLAGPTELARLSQELGADTPLSASLAAAILDWRTEGILPRPGGA
                     KTPQYRAAGLVYGPADGPFRSVSELGLVLGMPPALLERLRPHFTVFTAMEPVGLSSDP
                     IVSRAVQAVSPAGALPTGGDLGGIAVERITASGRGPDGAATVLEAVVRLNATASGLPF
                     EIFSWRRAGG"
     gene            complement(3801..5663)
                     /locus_tag="VKQ27_10305"
     CDS             complement(3801..5663)
                     /locus_tag="VKQ27_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLY89367.1"
                     /translation="MGTRDNQTTTPIKHVIVIYGENRSFDHLFATYRPRHGQSVRNLL
                     SEGIIDELGNPGPKAAKATQYQATDTTTYSIAPTKAGAYQVLPPVMTGGAATSASDTS
                     PPPFATAAAAAAADYGLLPSDIPKLTTGATGLPKDGVDTRRPNATSLPPYPYQLTPGI
                     GYDAYAASPVHRFYQAWQQSDCAVSQASFDNPSGCLNDLFPWVEVTIGAGSNGKAQPA
                     NFNDQSTGEGSTSMGFYNVLQGDVPYFKQLADQYTMSDNYHQPSMGGTGLDSIIAGFG
                     DAIWYTKNGQPATPPSNQIENPNPQSGTNNYYTQDGYSGGSYSACADTAQPGVGAVVS
                     YLQALPRKVAANCEAGHYYLLNNYNPGFFGDGSVDTTDTFAIPPVTTRSIGDALHADN
                     VSVTWFGEGWDQYKANPNDPTNVYCNICNPFQYETSIMTNPALRDAMIQDTKDFYADV
                     QQGVLPAVSLVKPSGLNDGHPSSSKYDIFEGFVRKIVTAVQSNPELWKSTAIFITTDE
                     AGGYYDSGYIQPLDFFGDGPRIQMIVVSPYSQGGNITHSYTDHVSILKFIERNWHVGP
                     ISSRSRDNLPNPIAAPFNPYVPINSPALGDLMDMFNFKSGHDHDHDHDDGQSDD"
     gene            complement(5931..6536)
                     /locus_tag="VKQ27_10310"
     CDS             complement(5931..6536)
                     /locus_tag="VKQ27_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLY89368.1"
                     /translation="MPHPAAHGSGFTLLEVLVTLVVLGLLMVTLTQGTRVGLRAWAMD
                     GNVAQRVNGLETTDRALRQLIERALPQDLRSNDGGLVGDAHALSFTTTLPEGYGARST
                     HEADVSLRVSSDHRLELLWRPHYSRWIVQPPAPSVIPLVDGVERLDVGYLEPGSQGRW
                     SAAWPKRALPPLVRLRIVFPAGDERHWPDIVIAPMRDPLAW"
BASE COUNT         1220 a         2270 c         2108 g         1111 t
ORIGIN      
        1 ggcgcccacc ggcattgccg cgggtgcgtt ccctggcatc ggcatggcac gcagttgcac
       61 atcgtcgacc aacatgcgcg gctcgccctg gctcgccact cgcagcagct cgaccagcac
      121 cggccacggc gcggtcagcg atacgcgaag ccctattctg cggtagccgc ctcgcgcttc
      181 cgccggcagc gtttccatgc tcgcgagctc gacgcccgca cgcgttgcca tcgcctgcac
      241 cgcattctgc atcgccgccg cggccagcgc atcggtcgcg ccggacagca gtggcgtgct
      301 ctcgcgccgc gccgcggcag gcgtctgttc cagcgtcggc aatgtctggg ccagggcctc
      361 catgtggcgc agaagcatct ggcgctgcac cagcgtatcc acgcccgact gataccagtc
      421 gagcagtggt tttccgacgc ccagccatgc ggctgccagc acggcgagcg tcagcgctgt
      481 cgcaaggaac tggccgcggc ggccttcggg gagggacatg tccatcttag ggggcaagat
      541 cagcgtggat cacgaacagg tcggtgcggc catcgggcgc acgcgtcacc ggcgccgcga
      601 aggccgggtt gcgcagggtg ggttccgccg ccagcgccgg tatgagccgc gccgcgttcg
      661 gcgactggcc ggcaatgttc aacttgccct ggtggagggt gaattccgtc agccaggcgt
      721 catccggaag gatgtcggta agagtagcca gaacctggat gacgttccct atacgccctc
      781 gctcggccgc cagcacgtcg ccgctcgcgt ctgtttgttg ccggcgcagc gcctcgaccc
      841 gcttcacttg cggctgcagt gcgacaatcg tgcgctcggt cgtgctgcgc gcgaaatact
      901 gcataacgaa tggcgttgcg atgacggcca cggccaacgc tgcaacgagg ctggcgccga
      961 tcccgaaaac acggctgggc ctgggcgcca cgtgatccgc cagcgcaatc cggcgcggcg
     1021 aaccatcggc ggccggcagc tccagccagc ccggcgccag cccggcccgc gccagcatgt
     1081 ccagccaggg ctgaatcgcg cgccgtggcg cgaggctgag ccgcagcacc aatcgctttt
     1141 gcgcgtgatc gcgcctcatg acagccgcct gccagacgac ctcgtcggcg gcaaacgggg
     1201 tcagccggtc catttcgaac ccgatcacac ggtcgatgtc ccgctccgcg gccaggggca
     1261 gctcgaccgg acgttccagc agcgtacccg gaccgagttg cagcaccacg cgccgcggca
     1321 gccgtcgcaa tgcccggacg gcgaccgcca gcccatcacc cgctgcacca aactgaccga
     1381 gcggattctc ccggccaccg cgacgcagcg tcagcaccac atgcgggccc cgcgccccaa
     1441 tcatcagcgc gtcgcctccc tcgccgccac gcaacaggcc ccaagggagc agcgcgtgca
     1501 tctgtcttgc ccaccacaag acgatttcac gcaacatgcc gcagccccgg tcgaactcgc
     1561 gaaagcatta acatggagga tcacgcacag acagcggacg aaaccataac gtcatccaac
     1621 tgccatggga atcccagatc gccagagtgc tccatcagcc gcccgccgcg gccaggtcgt
     1681 tgaggctgag catcgccgtc aacagcgagg cgacgatacc tgccaccgct gcccccatca
     1741 cgatggttat ggccggcacc atcagcgcga cgagccgctg caccgcaatc cgcgaccgct
     1801 cctcgtgaat ttccgccgcg tgcaatgcca tcgtgccaag ctgggcattc tcttcgccca
     1861 ggcgcagaag gtggatggtg cgcgccggaa acacgccgga ctcgccgagc ggttgcgcca
     1921 gactgccgcc gccacgcgca ctttgccctg cccgatcgag cgcaagcatc gcgacgcggt
     1981 tgcccagagc ctctcgcgcg atggccagtg ccccgatcag cggcacacca ttcaccagca
     2041 aagtgcccag ggcacgtgtc agccgcgcgg ccatcagttc gcgctgcaaa tgtccgatga
     2101 ccggcaatcg cagtatcagc ctgtcggcag ccaggcgcgg tcccggccgg cgcagcaggg
     2161 ccccgcacgc gagcaccagc ccgagccccg cgcacagcag cccgatgccc caattgctga
     2221 ggaaggcgcc ggcgtcgatc aggaactgtg tcgagcccgg aagtcgcgcg ccgctttgtt
     2281 cgaaaagcgg cacgaattgc ggcaggactt ctgtgagcaa cagggtcacg gcgcccacgg
     2341 cggctaccgc cagcaggcaa ggatagatca gcgctgaggt tacggtcgcc gcgacccggc
     2401 gctgccgctc cagcaactca gccaatcgcg ccagggcctc ggcaagccgc ccgctcgctt
     2461 cgcccgcacg gaccatgccg acatagagcc gcgagaagct cgccggctcg cgggccagcg
     2521 cctgcgacag cgggctgccg tcgcgcacgg cattgcggat gccttccagc acacgccgca
     2581 cgcgcgcgtt tggtgccgtc tcaagagcat agcgcagggc acggtcgagg tcctggccgg
     2641 cgccgagcat ggtcgccagc tcacgcgtca cgtcggcaat ctgctgtgcg gaaagcttct
     2701 gtcgcggcag gaaatcggcg ctgagcaacc cgcccaggaa gccaccgtcg ccgaccggtt
     2761 cggcacgcat cggcagactg ccctggcgct gcaggcgggc aacgacctcg gcctcgctgc
     2821 ctgcttccag cacaccacgg agcatttcgc cgccggcgcc gatggctgtg taacggaact
     2881 gcgccatggc gcactattgt cagcaccgcc gacgtcaagg caagtgaaag gctagcgaat
     2941 cgctgcaaca aatccgccca gcccttcagc caccggcgcg ccgccatgag aatatctcga
     3001 agggcagacc gctggcggtg gcattcaggc gaacgactgc ctcgagcaca gtggccgcgc
     3061 catccgggcc cctgccgctc gccgttatgc gttcgacggc gatgcctccc agatccccgc
     3121 ccgtcggcaa ggctcctgcc ggggatactg cctgcaccgc gcgcgaaacg atcgggtcgc
     3181 tcgacaggcc aaccggttcc atggcggtga ataccgtaaa gtgcggacgc agccgctcaa
     3241 gcaatgcagg cggcatcccg agaacaaggc cgagttcgga aacgctgcgg aatgggccgt
     3301 ccgccggacc gtacacgagg ccggccgcac gatattgcgg cgtcttggcc cctcccgggc
     3361 gcggcaagat cccctccgtc cgccagtcga gaatcgcggc ggcaagggag gctgacaggg
     3421 gggtatcggc tccaagttcc tgcgacagtc tcgccagttc cgtggggccg gcgagattag
     3481 ggttgagcag gtcgctttca ttggcgaccg ccaggtccac cagcgtcgca ccgatgcgaa
     3541 ccttacgtgg cgccgaaccg gcgccgctca gcaggtagaa tatctctccc tggatcgcac
     3601 cggcggtcgc cgcttcgacc tgcgcagcgg cccggctgtt ccgcgccatg gccatccacg
     3661 tccggccggt ggcgacaaag tgcgtttcga gcagtgccag gaacccgaga acaaacaaca
     3721 cgatcagcaa ggcgaaaccg cgctcgccag ggaggcattg cccccctggc gccattacgc
     3781 gattggacgg ttcacgcgcg tcagtcgtcg gactgcccat cgtcatggtc gtgatcgtga
     3841 tcgtggcccg atttgaagtt gaacatgtcc atcagatcgc caagcgccgg gctgttgatc
     3901 ggcacatagg ggttgaacgg cgccgcaatc gggttgggca agttgtcccg gctccggctg
     3961 gagatcggac caacatgcca gttccgctcg atgaacttca ggatcgagac gtgatcggtg
     4021 tagctgtgcg tgatgttgcc gccctgtgaa tagggcgaca ccacgatcat ctggatgcgc
     4081 ggcccatcac cgaagaagtc gagcggctgg atgtagcccg agtcatagta gccgccggcc
     4141 tcgtcagtgg tgatgaagat cgccgttgac ttccacagct cagggttgga ctggaccgcc
     4201 gtcacgatct tgcggacgaa accctcgaag atatcgtatt tcgacgatga aggatgacca
     4261 tcgttcaatc cgctcggctt gaccagcgag acagccggca gcacgccctg ctggacatcc
     4321 gcgtaaaagt ccttggtatc ctggatcatg gcatcccgca gcgccgggtt ggtcatgatc
     4381 gaggtttcgt actggaacgg attgcagatg ttgcagtaga cattggtcgg atcgttcggg
     4441 ttcgccttgt actgatccca tccctcgccg aaccaggtca ccgacacgtt gtccgcatgc
     4501 aatgcatcgc cgatgctgcg ggtggtgacc ggcggtatcg cgaaggtgtc cgttgtatcc
     4561 acggagccat caccgaagaa gcccggattg tagttgttca gcagataata gtgccctgcc
     4621 tcgcagttcg ccgcgacctt ccgtggcagc gcctggaggt aggagaccac cgcacccacg
     4681 ccaggctgcg ctgtatcggc gcacgcgctg tacgagccgc ctgaatagcc atcctgcgta
     4741 tagtagttgt tggtgccgga ctgcgggttc ggattctcga tctggttgct cggcggcgtc
     4801 gccggctggc cattcttcgt ataccagatt gcatcaccaa agccggcgat aatgctgtcc
     4861 agccccgtcc cgcccatgga cggctgatgg taattgtccg acatcgtgta ctgatcggcg
     4921 agctgcttga agtacgggac gtcgccctgc agcacgttgt agaagcccat cgaggttgag
     4981 ccttcacccg tgctctgatc gttgaaattg gccggctgtg ccttgccatt gctgcccgcg
     5041 ccaatcgtta cttcaaccca ggggaataga tcgttcaggc acccgctcgg gttgtcaaag
     5101 ctcgcttggc tgacggcgca atcggactgc tgccatgcct ggtagaaccg atgcacgggg
     5161 ctggcggcat aggcgtcgta gccgatgcca ggcgtgagct gatacgggta cggcgggaga
     5221 ctcgtcgcat tggggcgccg ggtatcaacg ccgtccttcg gcaatcccgt tgcaccggtg
     5281 gtcagtttgg ggatgtcgct cggcagcagc ccgtaatcag cagcggcagc agcagcagca
     5341 gtagcaaacg gcggcgggct tgtgtcgctc gcgctagtgg cggcgccacc cgtcatcacc
     5401 ggcggcagca cctggtacgc gccagccttg gtgggcgcaa tgctataggt cgtggtgtca
     5461 gtcgcctggt attgcgtggc cttcgccgcc ttcggtcccg gattgccgag ctcgtcgatg
     5521 atgccctcgg agagcaggtt gcgcacggac tggccatggc gaggacgata ggttgcaaac
     5581 agatgatcga agctgcgatt ctcgccatag atcacgatga catgcttgat cggtgtcgtc
     5641 gtctgattgt cgcgcgtgcc caccgcaggt gcagccaggg ccgcgggtgc aaaagcattt
     5701 aaaccgattg cgaaagccga tacgcaagca aatagccgac gccgccaggt gacggtggtc
     5761 atgccgaact cccctgttct gttttgaccg gggaccatat agcggggggt tctgactgat
     5821 cctttacagg aatgttacca gaaaatgacg gcgttgatca tgccgcaatt gtgatgttcc
     5881 tctgcgccgc gtcgccggcg cgcggtcccg gtatccgcgc ccaggcacca ctaccaagcc
     5941 agcgggtctc gcatcggagc aatgacaatg tccggccaat ggcgttcgtc gccggcagga
     6001 aacacgatgc gcagccggac caggggcggc aatgcgcgtt tcggccaggc agcgctccag
     6061 cgcccctgcg aacctggttc cagatacccg acatccaggc gctccactcc atccactaaa
     6121 gggatgaccg agggcgcggg cggctgcacg atccaccggc tgtaatgcgg acgccagagc
     6181 agctccagac gatgatcgga agacacccgc agcgacacat ccgcttcatg cgtcgagcgt
     6241 gcgccatatc cttccggcaa ggtcgtggtg aacgacagcg cgtgcgcatc gccgacaagc
     6301 cccccgtcgt ttgatcgcag gtcctgaggc aacgcgcgct cgatcaactg ccgcagggcc
     6361 cgatccgtgg tctcaagccc attcacgcgc tgcgccacgt tgccgtccat cgcccatgcg
     6421 cgcagcccaa cccgcgtccc ttgcgtcagc gtcaccatca acaggccgag gaccacaagg
     6481 gtcaccagga cctcgaggag cgtgaacccg cttccgtgcg ctgccgggtg cggcatggcg
     6541 gaagcgccat ttttcgcacg acggtcaccg ccccgccacc tcccgatgga tggcaggtcg
     6601 atgacagatc gctggagcag cgatctattg cgccgcgacc gtatcaacgt gacccgtcag
     6661 ccagttcact gccaccacca tgcggatgtt ggacgcctgc acgacaatt
//
LOCUS       DATGEM010000321         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_535155, whole genome shotgun sequence.
ACCESSION   DATGEM010000321 DATGEM010000000
VERSION     DATGEM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535155"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VKQ27_10315"
     CDS             complement(<1..1175)
                     /locus_tag="VKQ27_10315"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY89369.1"
                     /translation="MPASGGPRLFRRHRRRIIDERRTVVATDAVTIGLRRGRPSAALL
                     AHVLVAKYCDHLPLYRQSAMYARDGLDLDRSTLADWVGQAAWLLDPVVEGIRAHVFAA
                     DKIHGDDTPVPVLAPGTGKTKTGRLGVYARDDRPFCGTAPPAAAFFYSPDRKGERPHE
                     HLTDFTGFLQADAYAGYEALYDSGRTVPGPIVEVGCWAHCRRKYFDVWEATKSAVAKE
                     ALDRIAAIYVIEAEARDRPIGERVAIRAKACPLIDGFFDWGHAMLGKLSAKSALADAF
                     RDTLKRREALSRFLSDGRLETDNNRAENCLRGVALGRKNWTFAGSDAGGESAAASYTI
                     IETALCRARHNAVYAEGRTMPNGREGSRGKGRVARRRAAPVPATFGIVLRPTTLRGTR
                     "
     gene            1137..1649
                     /locus_tag="VKQ27_10320"
     CDS             1137..1649
                     /locus_tag="VKQ27_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89370.1"
                     /translation="MPAEEPGPSTRWQVIKMVAGWAAKRAAILAGEDIAGGPVGLLLN
                     AAQIASWVHDYGPYIQAYTDPPQSLEDLETAAQGPARRGYDVHHIVEQASGRSGEIPT
                     DLIDSEENLVSIPTLRHWELNSWYQTKNEDFTDAQGDPMAPRDYLTGKGYDERRRVGL
                     IGMRAVGILK"
     gene            1646..2008
                     /locus_tag="VKQ27_10325"
     CDS             1646..2008
                     /locus_tag="VKQ27_10325"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HLY89371.1"
                     /translation="MKLDKLTTDELVAQFVSLSEQQYLLMLNDDFKKFNHLFDKQTEI
                     DKELRKRGIEARLELTKLFNHPNIQVRLSAAKRSLGVARESALDVLRQIIKEDFGPFR
                     LRAGMTVALVEDGTVEPT"
     gene            complement(2380..2529)
                     /locus_tag="VKQ27_10330"
                     /pseudo
     CDS             complement(2380..2529)
                     /locus_tag="VKQ27_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2683..2758)
                     /locus_tag="VKQ27_10335"
     tRNA            complement(2683..2758)
                     /locus_tag="VKQ27_10335"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2723..2725),aa:Phe,seq:gaa)
     gene            complement(2909..>3645)
                     /locus_tag="VKQ27_10340"
     CDS             complement(2909..>3645)
                     /locus_tag="VKQ27_10340"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HLY89372.1"
                     /translation="VEEVGRPLHLLYPLLLLAIPAALVMKQPDLGTAMMLVLSTGGIF
                     FLAGVRLWKFAIAIAGAAGLVPIAFGFMRDYQKKRILTFLNPENDSLGAGYHITQSKI
                     ALGSGGMWGKGYLEGTQSHLNFLPEKHTDFIFTMFAEEFGMAGGCVLLALYALVVAYG
                     CAIGLRSRNHFGRMLALGVTLNFFLYVFINTAMVMGLIPVVGVPLPLISYGGTAMLTL
                     MFGFGLVISVYIHRDVRINRRGESES"
BASE COUNT          708 a         1164 c         1126 g          647 t
ORIGIN      
        1 cgcgttcctc gaagcgttgt aggccgcaag actatgccga atgtcgccgg gacaggggcc
       61 gctcgacgcc ttgcaactct gcccttgccg cggctaccct cccggccatt cggcatagtt
      121 cggccttcgg cataaacggc gttatgccgc gcgcggcata aggccgtctc gatgatggtg
      181 taactggcgg cagcgctttc gccgccggca tcgctgccag cgaaggtcca gttcttccgt
      241 ccgagggcaa cgccgcgcag gcaattctct gcacggttat tgtccgtttc gaggcggcca
      301 tcggagagga agcggctcag agcctcgcgc cgttttaggg tatcgcggaa ggcatcggcc
      361 aacgccgact tggccgacag cttccccagc atcgcatgcc cccaatcaaa gaacccatcg
      421 atcagcgggc aggcctttgc ccttatcgcg acgcgctcgc cgatgggcct atcgcgcgct
      481 tcggcttcga tcacgtagat cgccgcgatc cggtcgagcg cttctttggc gaccgccgac
      541 ttcgtcgcct cccacacgtc gaaatacttc cggcggcagt gcgcccagca gccaacctcg
      601 acgatcggtc cgggcaccgt ccggccggaa tcataaagcg cttcgtaccc ggcataggcg
      661 tcagcctgga ggaagccagt gaagtccgtc agatgctcgt gtggtcgctc gcccttgcgg
      721 tccgggctgt agaagaatgc cgctgccggt ggcgccgtgc cgcaaaacgg tcgatcgtcg
      781 cgggcataga ccccgaggcg gccggtcttg gtcttgccgg tgccgggtgc cagcacggga
      841 actggcgtgt cgtcgccatg gatcttgtcg gcggcaaaga catgcgcccg gataccttcc
      901 acgaccggat cgagcagcca ggcggcttgg ccaacccaat cggcgagggt cgagcggtcg
      961 agatcgaggc catcgcgcgc gtacatcgcg ctctgccggt agagcggcag gtgatcgcaa
     1021 tacttggcga ccagcacatg ggccagcagt gccgcactcg gccgaccgcg ccggagccca
     1081 atcgtcacgg catcggtggc aaccacggtc cgccgctcat cgatgatgcg ccgccggtgc
     1141 cggcggaaga gcctgggccc tccactcgct ggcaagtcat caagatggtc gctggctggg
     1201 cggcgaagcg cgccgcgatc ctggccggcg aagacatcgc cggcggcccc gtcggcttgc
     1261 tgctgaatgc ggcgcagatc gcctcgtggg tccatgacta cggcccctac atccaggctt
     1321 ataccgatcc gccgcagtcg ctcgaggatc tcgaaactgc ggcgcaagga ccggcgcgcc
     1381 gagggtatga tgtccatcac atcgtggagc aggcatcggg acgcagcggc gagattccga
     1441 ccgatctgat cgattcggag gagaacctcg tcagcatccc taccttgcgg cattgggaac
     1501 tcaattcctg gtaccaaacg aaaaatgaag attttaccga cgcccaaggc gacccgatgg
     1561 cgcctcggga ctaccttacc ggtaaaggct acgatgaacg tagaagggta gggctgatcg
     1621 gaatgcgagc tgtggggatt ttaaaatgaa actcgataag ctgacgaccg atgaactcgt
     1681 cgcccagttt gtgtcactca gcgagcagca atacctactg atgctcaatg atgatttcaa
     1741 gaaattcaat catctgttcg acaaacaaac cgagatagat aaggaattac gcaagcgggg
     1801 catcgaggcg cgcctcgaac taacgaaact ctttaaccat ccaaatatcc aagttcggtt
     1861 gagcgccgct aaacgtagcc tcggagtagc tcgggagtcc gctctagacg tccttcggca
     1921 gatcatcaag gaggattttg ggcctttccg cctgcgcgcc ggcatgaccg tcgctcttgt
     1981 tgaggatggc accgtcgagc cgacctgacg cctgtcatcc ttcaggcctt atagactgcc
     2041 gatcgagtgg atagacgagg atgccgtcga tcggcagcag ggcgtggctg tgagtagccg
     2101 aatgatttgg cacaaagcga ggccccgccg gtatcgtcgg gatcgcgacc aagcttagga
     2161 tactgttcgg accatgctca accggccgag gccgatccga tcagcgatcg aggtcgacgc
     2221 gccgaatccg ggtcctacgc ccgcagtcgc ccgctgacgc catgcacgct ctgcgcccgc
     2281 cttgcggcgg gcgtcagaac tttttgtggc gagatccttc atcacagctt gtcgagcatc
     2341 gcctcgacca acagcagtag gccgtcctca gcggcttcgc tccgacaagt cgaggccgtc
     2401 gtacttcgcc ttccggtcgg agaatgtcgt gtcgcagagg ccgcgcttgc ggcaaatacc
     2461 cgccgtcttc gcgcctatct cctgcccccg caggatcccg atgatctgct cttccgtgac
     2521 cgcgatcact ccatcgtccg cccccaggag aggcggactc tagatcaggc cggaggaatt
     2581 ttcagggatc aagtcacccg gttgttaccc caccgcatca gccggtcata agcgaccgac
     2641 gccgcttcgc cgctcaaacc gttgctgcgc ttgaaaggga aatggtgccc aggggcggaa
     2701 tcgaaccacc gacactgcga ttttcagtcg catgctctac caactgagct acctgggctc
     2761 cggggcaacc gaaggagcgc ttctataaga aatatccggg cgcctgtcca gcgccgcttt
     2821 gcatgcgggg gatgcgccgg aaaagcttgc gcttcggtgc gattctccga gaccggcacc
     2881 gcgcacccat cccggagaat cacgggtgtc agctttccga ctcgccgcgc cggttgatgc
     2941 gcacgtcgcg gtggatatag acgctgatga ccaggccgaa gccgaacatc agggtcagca
     3001 tcgccgtgcc gccgtaggaa atcagcggca gcggcacgcc caccaccggg atcaggccca
     3061 tgaccatcgc ggtgttgatg aagacataga ggaagaagtt gagcgtcacg cccagcgcca
     3121 gcatccggcc gaagtggttg cggctcctaa gcccgatcgc gcagccgtag gccacgacca
     3181 gcgcatagag cgcgagcagc acgcagccgc cggccatgcc gaattcctcg gcgaacatgg
     3241 tgaagatgaa gtcggtgtgc ttctcgggca ggaagttgag gtggctctgt gtcccctcca
     3301 gatagccctt gccccacatg ccgccggagc ccagcgcgat cttggactgg gtgatgtggt
     3361 atccggcgcc caggctgtcg ttctccggat tgaggaaggt caggatgcgc ttcttctgat
     3421 agtcgcgcat gaagccgaag gcgatcggca ccaggcccgc ggcgccggcg atggcgatgg
     3481 cgaatttcca caggcgcacg ccggccagga agaagatgcc gccagtcgac agcaccagca
     3541 tcatcgccgt gcccaggtcc ggctgcttca tcacgagcgc cgccgggatc gccagcagca
     3601 gcagcggata gagcaggtgc agcggccggc cgacctcctc gaccg
//
LOCUS       DATGEM010000322         8158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1051260, whole genome shotgun sequence.
ACCESSION   DATGEM010000322 DATGEM010000000
VERSION     DATGEM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051260"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..652)
                     /locus_tag="VKQ27_10345"
     CDS             complement(83..652)
                     /locus_tag="VKQ27_10345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLY89373.1"
                     /translation="MRLRCSLALMVLLPCTALAEPALRPEATLLPPVGSGCISSPFGP
                     RVLPNHPLAGTYHNGVDMPAPAGAAIRTVAPGTVIRVQRKGPGGLEILVQHDGFVGIY
                     SHLGMVTPAIAEGQHTLSAGEKIGVVGHSGVTYGMHVYFEILKNGHAVDPAPLLGLPL
                     CNGGVIRRPSDMLAADGKIPPTRHYAGLN"
     gene            complement(727..1824)
                     /locus_tag="VKQ27_10350"
     CDS             complement(727..1824)
                     /locus_tag="VKQ27_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY89374.1"
                     /translation="MKITAVETYWTRIPFDMGGKPATQGGLNWQTMNTVWLRVITDQG
                     LEGWGEGFGHASAAATKTVMDTQLAPLAIGQDARDVGGLRIRLAKALHGFGRNGSHSF
                     ALSALDIALWDIAGKAANLPLWRLFGATPVSHLTSYASLLRYGAAPMVSAACERAISR
                     GYRDIKLHEITVPEVRAARQAIGQDARLMVDTNCPWTVWEAIDIARRMREYDLTWLEE
                     PVSPPGDHKGLARVRREGGVPIAAGENAAGLHDFHAQFEAGAIDVAQPSVIKIGGPSA
                     MFEIAALAKVHGVRVVPHNAYFGPGFLASLHVNATIAPDAPFERLFIDLETSPLGDWV
                     VAKDGKVAVPAGPGLGCDPDMQILKRYLVSA"
     gene            1985..2500
                     /locus_tag="VKQ27_10355"
     CDS             1985..2500
                     /locus_tag="VKQ27_10355"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HLY89375.1"
                     /translation="MDLKEHIRGIPDFPKPGILFYDISTLLRDADAWQVAMGRMARGV
                     RAYQPDLLAGIESRGFLIAAPLALKLGCGFIMLRKRNKLPGVTVGLNYSLEYGTDRVE
                     VQADAVQPGQRVVIVDDLLATGGTMAAGIALLRQVGAVVPAAAALIELTFLNGRSRLD
                     VPCEALVAYDE"
     gene            2570..3634
                     /locus_tag="VKQ27_10360"
     CDS             2570..3634
                     /locus_tag="VKQ27_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89376.1"
                     /translation="MIDQSPLGRRLLLASLGTLPAIARVGRVRAAPSSAAFRDNPRLL
                     VAGPEDGALNRWADALQPALEQSLPSDTAIRRVEIGSADGVTGANQFEARGAPDGTTL
                     LLVPGQATLAWMVGDSRAQFDVGHWVPVMAGMTSGIAVGRPGVLTSGARVRVAAAGAA
                     SLDLAALLGLQLLGVPIQPVFGITDVATAHSAFTQNAVDAVFLRGQRVPDQFSVLAAS
                     GAQGLFTIGILGEAGQAARDPIFPDVEHFSELYQMRAGRAPSGLLFNAWRAVAAAAQL
                     DFGLVLQQLTPAAMVSLWRRAGADAAAALPVQAVGTSLSVRLVAAPDATTNTAAAVAS
                     ASTLTELRGWLAGQLNWKPA"
     gene            3631..4440
                     /locus_tag="VKQ27_10365"
     CDS             3631..4440
                     /locus_tag="VKQ27_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLY89377.1"
                     /translation="MKLWYQSMSRQSEWGSYPRILRGVLDHVRDPDTEIHVAGITEIG
                     GTGDQYRYLEYLETGEVLKNVHRAVREGFDAFLIGNIADPGLQEAREIADIPVLGLCE
                     SALHVACMMGARFSLVTINEKFTPRIVENVWRYRLQDRMAAVNRMQVERLTDLGAGFE
                     PGPSRDAIVTQFRRAAKANAEAGAEVVIAAGGVVMALLAHAGIHEAAGLPILNGITNL
                     VKLGEMAVKTNRLMGGRFTSKRCTYAPPPAGQIAEFRKYYGADIYPTVKEG"
     gene            4440..5273
                     /locus_tag="VKQ27_10370"
     CDS             4440..5273
                     /locus_tag="VKQ27_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89378.1"
                     /translation="MLRLRTALGKSPLIRALKGGTVSSDRVGFEFVEVDPITRAFRRQ
                     TRGMEFDLCEIALTTHAQARAYDKPITALPVVLLRGLHHGALMCRADSPLRGPAALVG
                     KRIGVRAWSQTTGVWMRGVLHDEYGIAPSSMTWITEEDAHVQEFTDPPHVERMAQGQD
                     LRGLLLSGAVDAVIAVAGLDPADVRTVIPDADAVAQEWSRKTGVFPINHVVVVKDALL
                     AAHPWLADELMRLFVAAKGDAPVPYGVEANRPAIELLMRYASEQGLIPRPFRVEELFA
                     T"
     gene            5796..6992
                     /locus_tag="VKQ27_10375"
     CDS             5796..6992
                     /locus_tag="VKQ27_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLY89379.1"
                     /translation="MSADFIRTDGAARALRIIAIVSAAALLVWALANVVLLVFMAALL
                     AIILRGISGWLADLTGAPLKLMLAFVSLGILAFLLALLYFIGPSLVTQTQALFAQLNQ
                     TFDRLRQTYGNTGWGHLLFQHLSPGELLNNSIFGSAGALASSTLGGVVSAFVLVVTAM
                     YFAVDPDLYVDGIVRLFDIPHRPRARIALEHVGRTLKLWSLGQLIDMIAVGVLTVIGL
                     TVLGLPLALALAVLAGLLTFIPYFGAIAAAIPAMLIAFAADWRKAIWVAVIFTIAHIA
                     EGYIIAPLVQRNTADLPPAVTILSMTILGTLFGTLGVILGAPVSAAVLVLVREVYVGG
                     VLGDADVVPQPCVVLASSLVTVAHHDVDRLPGLGGHPAPEAVRTGATGMRAMRPRAGE
                     PHGTAP"
     gene            6961..7659
                     /locus_tag="VKQ27_10380"
     CDS             6961..7659
                     /locus_tag="VKQ27_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HLY89380.1"
                     /translation="MLENRTGRRLDRALACVIAVLLLLIPANTLPLMTVHLGPVTSSK
                     VLASGLCIAWAQGWPLVRIVLALEAVILPFLRFGLLSVTLAAIRSSHHDRWVGRAFRY
                     SEMLDLWAMTDVLLIGAGIGYGRLAAQTSVTIDAGGWCFVCVAVMTMITRGTLEASGS
                     LAQIGTGTFGRTVLGHRLHRMRPAAARCRRWQALSALLRKGSSAASGLVEHMLRAADR
                     HCRLDAARLWLPDE"
     gene            7652..8041
                     /locus_tag="VKQ27_10385"
     CDS             7652..8041
                     /locus_tag="VKQ27_10385"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HLY89381.1"
                     /translation="MSEFWKGEQPSPHTVINGIILLFTSGFWYFGVIIFFVSVVFPLS
                     KLAALAWFLTSIHRRSRWKLRLKAKVYRFIDQIGRWSTLDPFTVLVFVPMIQFGQLAH
                     FDFMGGAAAFLATVVLSMVATRALAPV"
BASE COUNT         1307 a         2437 c         2776 g         1638 t
ORIGIN      
        1 tggccgggca aatcatacaa tttggttaag cgccaacagg cgccgcgctg aacgcagcga
       61 gccgcaaccc gtcggtgagg atttagttca gtccggcgta gtggcgtgtc ggcggtatct
      121 tgccgtccgc cgccaacatg tcgctcgggc ggcgaatcac gccaccattg cacagcggca
      181 ggccaagaag cggcgctgga tcgaccgcgt gcccgttctt caggatctcg aaatatacgt
      241 gcatgccata ggtcacgccg ctatggccca ccacgccgat cttttcgccg gccgacaggg
      301 tgtgctggcc ctcggcgatc gccggcgtga ccatgccaag gtggctgtag atgccgacga
      361 agccatcgtg ctgcactagt atctccagcc ctcctggccc tttgcgctgc actcggatca
      421 ccgtgccggg tgccaccgtg cggatcgcgg cacccgcggg cgccggcata tcaacgccgt
      481 tatgatacgt gccggccagg ggatggtttg gcagtacgcg cgggccaaac ggcgacgata
      541 tgcagcccga gccaaccggt ggcaggagcg ttgcctccgg ccgaagtgcg ggttcggcga
      601 gagccgtaca cggcagcaag accattagag cgagcgaaca gcggagccgc atgacggtta
      661 gatcaggcac gatgctgcgc gcccgtccag tcgaatcgcc gtgtctccag acacgacacg
      721 gtgcgctcac gcggatacca ggtagcgctt caggatctgc atgtcggggt cacagcccag
      781 gcccggcccg gccggcaccg ccaccttgcc gtccttcgcc acgacccagt cgccgagtgg
      841 gcttgtttcg agatcgatga acaaccgctc gaagggcgcg tcgggcgcga tagtggcgtt
      901 cacgtgcagg gaggcgagga agcccgggcc gaaataggcg ttgtgcggca ccacgcgcac
      961 tccgtgcact ttggcaagcg ccgcgatctc gaacattgcg gatggaccgc cgatcttgat
     1021 cacgctcggc tgcgcaacat cgatggcgcc ggcttcgaat tgcgcgtgga agtcgtgcag
     1081 gccggcggca ttctcgcccg cagcgattgg cacaccgcct tcgcggcgga cgcgcgcgag
     1141 gcctttgtgg tcgccgggcg gggagacagg ctcctccagc caggtcaggt catactcgcg
     1201 catccggcgc gcgatatcga tcgcttccca gaccgtccag ggacagttgg tatccaccat
     1261 cagccgcgcg tcctggccga ttgcctgccg tgcagcgcgc acttccggca ccgtgatctc
     1321 gtgcagcttg atgtcgcggt agccgcggct gatcgcgcgt tcgcaggctg cactgaccat
     1381 cggggcagca ccatagcgga gcaagctggc ataggacgtg aggtgcgaca caggcgttgc
     1441 gccgaacaat ctccacagcg ggaggtttgc tgccttgcct gcgatgtccc acagcgcgat
     1501 gtcgagcgcc gacagtgcga acgaatgtga gccgttgcgg ccaaagccat ggagcgcttt
     1561 tgcaaggcgt atgcgcaggc cgccgacgtc gcgtgcgtcc tggccaatcg ccagtggcgc
     1621 gagctgtgtg tccatcacgg tcttggtcgc tgccgcagag gcgtggccga agccttcgcc
     1681 ccagccctcc aggccctggt cggtgatgac gcgcagccac accgtgttca tcgtctgcca
     1741 gttgaggccg ccctgggttg ccggcttgcc gcccatgtcg aatggaatgc gggtccagta
     1801 ggtctcgacg gcggtgatct tcatggcgtt tccctcacgg cggaagagcc gagcttaatc
     1861 ggcgcgcagc aataagagaa attccaacgg caggcgtcgt ctgcggtgaa ttgccgcgtc
     1921 tcttctcgtt gggaagccga tgctctacat cggctgatct attctctttt ggtaaggccg
     1981 cagcatggac ctgaaggagc acatccgggg cattccggat tttcccaaac cgggcatcct
     2041 gttttacgac atctccacgc tgctgcggga cgccgatgcg tggcaggtgg cgatggggcg
     2101 catggcgcgt ggggtgcggg cctatcagcc ggatctgctc gccgggatcg agagccgcgg
     2161 ctttctgatc gccgcgccgc tggcactgaa gcttggttgt ggattcatca tgctgcggaa
     2221 gcgcaacaag ctgccgggcg taacggtcgg gctgaactac tcgctggaat acggcactga
     2281 tcgcgtcgag gtgcaagccg acgcggtgca gccgggtcag cgggtggtca tcgtcgatga
     2341 cctgctggcc acaggcggca caatggccgc aggcatcgct ctgctgcggc aggttggcgc
     2401 agtcgtgccg gctgcggcgg cgctgatcga gctgacattc ctgaatggac gctcacgcct
     2461 ggatgtgcct tgcgaagcgc tcgtcgcgta tgacgagtga tcgaaccaag gcggctgatt
     2521 tgactcctgg tgggttgtga gtaggaagtt tgccccttgg ggggcgagaa tgatcgacca
     2581 gtcgccgctc ggccggcgtc tcctgcttgc gagcctgggc accctgcccg ccattgcgcg
     2641 ggtgggacgc gtgcgtgccg ccccgtcctc ggccgccttc cgtgacaatc cccgcctgct
     2701 tgtcgccggc ccggaagatg gtgcgctgaa ccgctgggca gacgcgctgc aacccgcgct
     2761 cgagcaatcg ctaccgtccg atacggctat tcggcgcgtt gagattggca gtgccgacgg
     2821 cgtcactggt gccaaccagt ttgaggcgcg aggcgcacct gatggcacga ccctgttgct
     2881 ggttcctggg caggcgacat tggcctggat ggtgggcgat tcgcgggcgc agttcgacgt
     2941 cggtcactgg gtcccggtga tggccggcat gacgtccggc atcgcggttg gccgtcccgg
     3001 cgtgcttact agcggagcgc gggtgcgcgt tgccgcagct ggagcggcga gcctcgatct
     3061 ggcggcgttg ctgggcctgc aattgctagg agtgccaatc cagccggtgt tcggcatcac
     3121 cgatgtggca acagctcata gcgccttcac gcagaatgcg gtggacgcgg tgttcctgcg
     3181 cggtcaacgc gtacccgatc agtttagtgt tctggccgca tctggcgctc agggcctgtt
     3241 caccatcggt atcctgggcg aggcggggca agcggcgcgc gacccgatct ttcccgacgt
     3301 cgagcatttt tctgaattgt accagatgcg tgcgggcagg gctccaagcg ggctcttgtt
     3361 caatgcctgg cgcgcggtgg ccgccgcggc gcagctggat ttcgggttgg tgctgcaaca
     3421 acttaccccg gcggcaatgg tctcgctgtg gcggcgcgcc ggggccgatg ctgcggcggc
     3481 gctgccagtg caagctgtcg gcacctcgct gagcgtgcgg ctggtggcgg caccggatgc
     3541 cacaaccaac accgctgcag cggttgccag cgcatccaca ttgaccgagc tgcgtggttg
     3601 gctggcgggg cagctcaact ggaagccggc atgaagctct ggtaccagag catgtcgcga
     3661 caaagcgagt gggggagtta tccgaggatc ctgcgtggcg tcctggacca tgttcgcgat
     3721 ccggacaccg agatccacgt ggctggcatc accgagatcg gtggcacagg cgatcagtat
     3781 cgctacctgg aatatctcga gacaggtgaa gtgctgaaga acgtgcaccg cgcggtgcgc
     3841 gaggggttcg acgcgtttct catcggcaat atcgctgatc ccgggctgca ggaggcacgc
     3901 gagatcgctg atataccggt gctggggcta tgcgagtcgg cattgcatgt cgcatgtatg
     3961 atgggcgcgc ggttttcgct ggtgacgatc aacgagaagt tcacgccgcg tatcgttgag
     4021 aatgtctggc ggtatcggct gcaggatcgg atggcggcgg tcaaccgtat gcaggtggaa
     4081 cggctgacgg atctcggtgc gggtttcgag ccggggccgt cgcgcgatgc gatcgtgacg
     4141 cagttccggc gcgcggcgaa ggcgaatgcc gaggctgggg cagaggtggt gatcgccgcc
     4201 gggggtgtgg tgatggcgct gctggcacat gccggcatcc acgaggcggc cggtctgccg
     4261 atactgaatg gcatcaccaa cctggtgaag ctgggcgaga tggcggtgaa aactaaccgg
     4321 ttgatgggcg ggcggttcac gtcgaagcgc tgcacttatg cgccgccgcc ggcagggcag
     4381 atcgccgagt tccgtaagta ctacggggca gatatctatc cgaccgtgaa ggagggctga
     4441 tgctccggct ccgcaccgcg ctgggcaaat cgccattgat acgcgcactg aagggcggca
     4501 cggtgtcctc ggatcgggtc gggttcgagt tcgtcgaggt tgatccgatc acgcgcgcgt
     4561 tccggcgtca gacgcgcggg atggagttcg acctgtgcga gatcgcgctg accacacatg
     4621 cgcaggcgcg cgcatatgac aagccaatca cggcgctgcc ggtggtgctg ctgcgcggct
     4681 tgcatcatgg cgcgctgatg tgccgggcgg attcgcctct gcgcgggccg gcggccctcg
     4741 tgggcaagcg catcggcgtg cgtgcgtggt cgcagacgac gggcgtgtgg atgcgaggcg
     4801 tgctgcacga cgagtatggt atagcgccga gctcgatgac gtggatcacc gaggaagacg
     4861 cgcacgtgca ggagttcacc gatccgccgc acgtggagcg catggctcaa gggcaggacc
     4921 tgcgcggttt gttgctgtca ggtgcggtcg atgcagtgat cgcggtggcc gggcttgatc
     4981 cggcagacgt gcgcaccgtg atcccggatg cggatgccgt tgcgcaggaa tggtcgcgca
     5041 agaccggcgt gtttccgatc aaccacgtgg tggtggtgaa ggatgcgctg ctggccgcgc
     5101 acccttggct ggctgatgaa ctgatgcggc ttttcgtcgc cgccaaagga gatgcgccgg
     5161 tcccgtatgg cgtcgaggcg aatcgccctg cgatcgaact gctgatgcgc tatgcctccg
     5221 agcaaggact gattccacgg ccatttcgcg tcgaggaatt gttcgccacg tagcgcaggc
     5281 gctccgagcg ctcagagttc ggaagagatc cttgtcggcg agttgcgcga gcgtgcggct
     5341 catgcctgac gcgatggcgt tgccgctagg gccatcagaa gcagtttgaa tgcggacgtg
     5401 ccgtatggcg ctggtggttt tcctgccatg cagcgaccaa tccgcttaca gggtcacctc
     5461 cccagtgggg accgcaacaa actgctccac gttcagagta agcgatcgcg cgtccggcgg
     5521 cgcaggatcg ccggcagtga gcaccgttcg gctctggtgc tctacagcat ccggcggttc
     5581 gtcttccgac tcgctcattc ggcgctgtaa cgccacggcg gcgcggccag ttccgaaggc
     5641 acatcgcgag tccgcggtgc ttcggctatg cgaccttcaa ctttcgcggc gcggacggca
     5701 cggatgcgtc caatctgtgt tgacggatca cagacaccgg catgtcggca caccacattg
     5761 gcacatctca gatcggttcg tcggactgca gaccgatgag cgctgacttc attcgtacgg
     5821 atggcgccgc gcgagcactg cggatcatcg cgatcgtctc ggccgcggcc ctgctcgtgt
     5881 gggcgctcgc caacgttgtc ttgttggtgt tcatggcggc gctgctggcg atcatactgc
     5941 gtggcatcag cggctggctg gcggatctga caggagcgcc gttgaagctg atgctggcct
     6001 ttgtcagcct cgggatttta gcctttctgc tggcactgct gtatttcatt ggcccgagtc
     6061 tggtgactca gacacaggcg ctattcgcgc aactcaacca aacttttgat cgcctccggc
     6121 aaacgtacgg caacactggc tggggacatt tgctcttcca gcatctgtcc ccaggcgaac
     6181 tgctcaacaa tagtattttc ggcagcgcgg gtgcgttggc cagctcgacg cttggcggcg
     6241 tggtaagtgc tttcgttctt gtcgtcacgg ccatgtattt cgctgttgat ccggaccttt
     6301 acgtggacgg tattgtccgc ctgttcgaca ttccgcatcg gccgcgcgcg cgaatcgcgc
     6361 ttgaacatgt ggggcgcaca ctgaaactat ggtcattggg ccagcttatc gacatgattg
     6421 ccgtgggagt gctcacggtg atcggcctga ccgtgcttgg actgccgctg gcgctggccc
     6481 tggcggtgct ggctgggttg ctgacgttca ttccgtattt cggcgcgata gccgctgcca
     6541 ttcccgcaat gctgatcgcc ttcgccgctg actggcgaaa ggccatttgg gttgcggtca
     6601 tcttcacgat cgcgcacatc gccgaaggtt acatcatcgc cccactggtg cagcggaaca
     6661 cggccgacct gcccccggct gtgaccattc tgtcgatgac gatactcggg acgctgttcg
     6721 ggacactcgg cgtgatcctc ggggcgccag tctcggcggc ggtcctggtc ctggtgcgcg
     6781 aggtctatgt tggcggcgta ttgggcgatg ccgatgtggt gccgcagccg tgcgtagtgc
     6841 tggcatcgag ccttgtcacg gtagcacacc atgacgttga tcgcctgccg ggattgggcg
     6901 gccatccagc gcctgaagcc gtccgaacag gggcgactgg aatgcgtgcg atgcggccgc
     6961 gtgctggaga accgcacggg acggcgcctt gatcgcgcgc ttgcctgcgt gatagcggta
     7021 ctcttgctgt tgataccggc gaacactctg ccgctgatga cggtgcatct ggggccagtg
     7081 acgagttcga aggtgctggc ctcgggtttg tgcattgcct gggcgcaagg ctggccgctg
     7141 gtcaggatcg tgctcgcgct agaagccgtc attcttcctt tccttcggtt tggactcttg
     7201 tctgtcacgt tggcagcgat ccgctcgagc catcatgatc gatgggtggg tcgtgcattc
     7261 cgctactccg agatgctgga tttgtgggcg atgaccgatg tcctgctgat aggtgcggga
     7321 atcggctatg gccggctcgc ggcgcagact tccgtgacga ttgatgctgg cggctggtgc
     7381 tttgtctgcg tggcggtgat gacgatgatc acgcgcggta cgctcgaagc atcgggaagt
     7441 ctggcgcaga ttggaacagg aacctttggc cgtaccgtcc tgggccatcg cctgcacaga
     7501 atgcgacctg ctgctgcccg ctgtcgccgt tggcaagcgc tgtccgcgct gctccgcaag
     7561 ggttcatcgg cggcgtccgg gctcgttgag catatgctcc gcgctgctga tcgccactgt
     7621 cgccttgacg ccgctcgcct atggcttccc gatgagtgaa ttctggaagg gcgagcagcc
     7681 ctctccgcac acggtgataa acggcatcat actgctgttc accagcggct tctggtattt
     7741 cggggtgatc atatttttcg tctcagtcgt gttcccgctg agcaagctgg cggcactggc
     7801 atggttcctg acatcgatcc accggcgatc gcgctggaag ctgcgtctga aggccaaggt
     7861 ctatcgattc attgaccaga tcggccgctg gtcaactctg gatccgttca ccgtgttggt
     7921 gttcgtgccg atgatccagt tcggtcagct tgcgcatttc gacttcatgg gaggcgcggc
     7981 agccttcctt gccactgtcg tgctgtcgat ggtcgccaca cgggcgttgg cccccgtctg
     8041 atgtgggatg cggcggccga ccagaagagc gctgtcactc cggttacggc ggggagcgcg
     8101 agagcctgag acccctcgtc agccgtggac tggtggccta tggtctgcgg gcgatcat
//
LOCUS       DATGEM010000323         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_98959, whole genome shotgun sequence.
ACCESSION   DATGEM010000323 DATGEM010000000
VERSION     DATGEM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98959"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VKQ27_10390"
     CDS             complement(<1..877)
                     /locus_tag="VKQ27_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HLY89382.1"
                     /translation="MTYIAKPKLHHPQLPANNLGFTRRDYEGAVSTLCAGCGHDSISA
                     AIIQACFELDLPPHRIAKLSGIGCSSKTPTYFLSSSHGFNSVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHSIRRGVNMVYIVENNGVYGLTKGQFSATSDRGSKS
                     KKGAINTDSAIDMVLLAIELGATYVARSFSGDKAQLVPLIKGAILHRGAAFIDCISPC
                     VAFNNHPGSTKSFDYVREHNDAVNHLDVMLPRETITADYAPGEVEEVTQHDGSVLKLR
                     KLAAEYDVHDRIAAMN"
     gene            complement(908..2740)
                     /locus_tag="VKQ27_10395"
     CDS             complement(908..2740)
                     /locus_tag="VKQ27_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HLY89383.1"
                     /translation="MSQPKINDFVVKFANINGSGSASANQLFAKSILRMGVPVSPRNI
                     FPSNIQGLPTWYEVRVSEAGYLGARGGVDLMVAMNPQTWDKDVFGIDPGGYLFYDSTK
                     PLPKSKFRDDIRVIGVPLTEICNREYSDPRQRQLFRNIIYVGALAALLEIDIAEIEKL
                     IGEQFQGKDKLIAPNIRALHLGRDYTRTHFECPIGLSVRRASAVENRIFIDGNTAAAL
                     GCVYGGATVAAWYPITPSSSLAEAFARHCRSYRRDGAGNSRYAVIQAEDELASIGMVI
                     GASWNGARAFTCTSGPGISLMQEFIGLAYFAEIPAVIFDVQRGGPSTGMPTRTQQGDV
                     RACAYASHGDTKHVLLFPEDPAESFDFAADAFDIAERLQTPVFVMLDLDIGMNDRLSR
                     PLAWNDARRYDRGKVATFEDLESGKEFGRYLDVDGDGITYRTYPGTHPSRGSFFTRGT
                     TKDRYARYSEAGPDYLDNMQRLLRKFESAKDVVPKPLVRQALQPSRLGVIYYGSTSPA
                     MHEALDTLEQRGLHFDAMRVRAFPFHDQVVDFIAEHDHVFVVEQNRDAQLRMLIVNEC
                     EIDPAKLTPVLHYDGTPITARFIVKEIAEDAAALKVTPMRKVLP"
BASE COUNT          514 a          863 c          908 g          535 t
ORIGIN      
        1 agttcatcgc ggcgatgcgg tcgtggacgt cgtattcggc ggcaagcttg cgcaatttca
       61 gaaccgagcc gtcgtgctgg gtgacctcct cgacctcgcc cggcgcatag tccgccgtga
      121 tcgtctcgcg cggcaacatc acgtcgaggt ggttgaccgc atcgttgtgc tcgcgcacgt
      181 agtcgaagct cttggtcgag ccgggatgat tgttgaacgc cacgcagggc gaaatgcaat
      241 cgatgaacgc cgcgccacgg tgcaggatcg cgcccttgat cagcggcacc agttgcgcct
      301 tgtcgccgga aaagctgcgc gcgacgtaag tggcgccgag ttcgatggcc agcaggacca
      361 tgtcgatggc cgaatcggta ttgatcgcgc ccttcttgct cttcgagccg cggtccgacg
      421 tcgccgagaa ctggcccttc gtcagtccgt agacgccgtt gttctcgacg atatagacca
      481 tgttcacgcc gcggcggatg ctgtgcgcga attgcccgag tccgatcgac gcggaatcgc
      541 cgtcgcccga tacgccgagg taaatcagtt cgcggttcgc caggttggcg ccggtcagca
      601 ccgacggcat gcgcccgtgc acgctgttga agccgtgcga actgctcagg aaatacgtcg
      661 gcgtcttcga cgagcagccg atgccggaca gcttggcgat gcgatgcggc ggcaagtcga
      721 gctcgaagca ggcctggatg atcgcggcac tgatcgaatc gtggccgcag ccggcgcaga
      781 gcgtggacac cgctccttca taatcgcgcc gcgtgaatcc cagattgttg gcgggaagtt
      841 gcgggtggtg aagcttcggc ttggcgatgt aagtcatggc agcgtccggg ttatcgggat
      901 gttccgctca gggtagaacc ttgcgcatcg gcgtcacctt cagggcggcg gcgtcctctg
      961 cgatttcctt gacgatgaag cgcgcagtga tcggcgtgcc gtcgtagtgc agcaccgggg
     1021 tgagtttggc cggatcgatc tcgcactcgt tgacgatcag catgcgcaat tgcgcgtcgc
     1081 gattctgctc caccacgaac acatgatcgt gctcggcgat gaaatccacg acctgatcat
     1141 gaaaggggaa tgcgcgcacg cgcatcgcgt cgaagtgcaa accacgctgt tcgagcgtat
     1201 cgagcgcttc gtgcatcgcc ggactggtcg agccgtagta gatcacgccg agcctgctcg
     1261 gctgcagggc ctggcgaacc agcggcttcg gcactacatc cttggccgac tcgaacttgc
     1321 gcagcagccg ctgcatattg tccaggtaat cggggccggc ttcgctgtag cgcgcatagc
     1381 gatccttggt ggtgccgcgc gtgaagaacg agccgcgcga tggatgcgtg cccggatagg
     1441 tgcgataggt aatgccgtcg ccgtcgacgt ccagatatcg cccgaattcc ttgccggact
     1501 cgagatcttc aaaggttgca accttgccgc gatcgtaacg gcgggcgtcg ttccacgcga
     1561 gcggccggct caggcgatcg ttcatgccga tatccaggtc cagcatcacg aacacgggcg
     1621 tctgcagccg ctcggcgatg tcgaacgcgt cggctgcgaa gtcgaagctt tcggccgggt
     1681 cttccgggaa cagcagcaca tgcttggtat cgccatgcga ggcatacgcg caggcgcgca
     1741 cgtcaccttg ctgcgtgcgg gtcggcatgc cggtggaggg tccgccacgc tgcacgtcga
     1801 aaatcaccgc gggaatctcg gcaaaatagg ccaggccgat gaactcctgc atcagcgaga
     1861 tgccgggacc ggacgtgcag gtaaaggccc gcgcgccgtt ccagcttgcg ccaatgacca
     1921 tgccgatcga ggccagctcg tcctcggcct ggatcaccgc atagcgacta ttcccggcgc
     1981 catctctgcg atagcttcgg cagtgccggg caaacgcttc cgccaggctg gacgacggcg
     2041 tgatcggata ccacgccgca acagtcgcgc cgccatacac acagcccagg gcggccgcgg
     2101 tattgccgtc gatgaatatg cggttttcca ccgcggaagc acgccgcaca ctcagtccga
     2161 tcgggcactc gaagtgggtc ctggtgtaat cgcgcccgag gtgcaacgcg cggatgttcg
     2221 gcgcgatcag cttgtccttg ccctggaatt gctcgccgat cagcttctcg atttcggcga
     2281 tgtcgatctc gagcagcgca gccagggcac cgacatagat aatgttgcgg aacagctggc
     2341 gctggcgggg gtcggaatac tcccggttgc agatctcggt cagcggcacc ccgatcacgc
     2401 gaatgtcgtc gcggaatttc gatttcggca gcggcttggt ggagtcgtag aacaggtaac
     2461 cgccgggatc gatgccgaac acgtccttgt cccaggtctg cggattcatc gccaccatca
     2521 ggtcgacgcc gccgcgcgca cccaggtaac ccgcttccga aacacgcact tcgtaccagg
     2581 tcggcagacc ctggatgttg gagggaaaga tgttgcgcgg gctgaccggc acgcccatgc
     2641 gcaggatgga tttcgcgaac aactggttcg ccgaggccga gccggagccg ttgatgttcg
     2701 cgaacttgac gacgaagtcg tttattttgg gctgcgacat gccggccccg cgtgcgtgac
     2761 attgagtaga aatttgcgca tgtcccacgc gccggtcggg caacgttccg cacacaatcc
//
LOCUS       DATGEM010000324         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_309523, whole genome shotgun sequence.
ACCESSION   DATGEM010000324 DATGEM010000000
VERSION     DATGEM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309523"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VKQ27_10400"
     CDS             complement(<1..315)
                     /locus_tag="VKQ27_10400"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLY89384.1"
                     /translation="MGVLNVTPDSFSDGGDFLDTDAAIAAGLAMVAAGADIVDVGGES
                     TRPSSHHTSPEVEQARIVPVIRVLTAAGVHVSVDTRNAATMAAALDAGAAIVNDVYAL
                     GFD"
     gene            complement(908..2836)
                     /gene="ftsH"
                     /locus_tag="VKQ27_10405"
     CDS             complement(908..2836)
                     /gene="ftsH"
                     /locus_tag="VKQ27_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLY89385.1"
                     /translation="MSNFGRNLALWVIIALLLVVLFNLFQPGSNHTAATQVAYSDFIN
                     EVNGGRVRDVVIQGRTVSGQLTDGRTFQTYTPEDSTLVQRLTDKGVRVIAKPDDDGAN
                     PLLHYLLSWFPMLLLIGVWVFFMRQMQAGGGRAMGFGKSRARMLTEKQGRVTFDDVAG
                     IDEAKGELQEIVEFLKDPQKFQRLGGKIPKGVLLVGPPGTGKTLLARAIAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQMLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPNPDVNGREKI
                     LRVHMRKVPLASDVDPKVIARGTPGFSGADLANLVNEAALLAARIGKRVVAMAEFEAA
                     KDKVMMGTERRSLVMSEAEKRMTAYHEGGHALCAMHEPECDPVHKATIIPRGRALGLV
                     MSLPEGDRYSKSKSKLLAELTMAMGGRAAEEIIFGPDKVSNGAAGDIKMATDQARRMI
                     TEWGMSDKLGMIAYGDNSQEVFLGHSVTQAKNVSEATAREIDAEIKDIIDRAYAKARR
                     ILTENIEELHRLARGLLEHETLTGDEIRTVLRGEPVIRKVVDEPAPESRRASVPTTGR
                     PAAPTPPAGGLGPAPQPG"
BASE COUNT          544 a         1068 c          862 g          539 t
ORIGIN      
        1 atcgaagccc agtgcataga cgtcgttcac gattgcagcc ccggcatcca gcgccgctgc
       61 catcgtcgca gcattgcgtg tatcgacaga cacatggaca ccggcggcag tcagcaccct
      121 gatcacaggt acgatacgcg cctgttcaac ctccggagac gtgtggtgcg acgatggccg
      181 cgtgctctcg ccacccacat cgacaatgtc cgcgccggcc gccaccatcg ccaatccagc
      241 cgcgatggcc gcgtcggtat cgaggaagtc tcccccgtcg ctgaaactgt ctggcgttac
      301 gttgagcacg cccatcactc gcggacgatc gagcgtaagc ccagcccaac actttacctc
      361 cgccatgggc ataccagaac cagactgccc tcagtgcctt cccgcagaac ccgcatgtca
      421 ggagtgcagc ttgtcaccga tattccaaat agccaggctg gccgcaccat cctcccgctc
      481 catcacgcac tggcacatca gcctatagca gatcagggct ccgcgacgtg gacactaatt
      541 gtcggcgacc gtgatggccc actgcagtat ctgtcgggaa atggcgcgcc atacttcgcg
      601 ggatgctggc atcgtctcat gagtcagaag aggcacggcg tccaatacac cgcaaggggt
      661 gaaacagttg acgttctgcg cgccaggtcg ccccgtccgg acgaacccgt cgatgatgaa
      721 gatctggcgg cgccccacgg tcgccagttc agacactgca ccggctgagt gcgcgacgac
      781 gacagcacac ggcctcagcg cgttcggtga taccgtcatt tcgtagagcg ccacggtcca
      841 ggctttcagg ctgacgttgg gtggtcactc agtgaccacc ccccgacaat gggcgcggcg
      901 tgccgcgcta acccggctgc ggtgccggcc ccaatccacc cgccggcggc gtcggcgcag
      961 ctggccgccc tgtcgtcggt accgacgcgc ggcggctctc cggcgccggc tcgtcgacca
     1021 ccttgcggat taccggctcg ccacgcagca ccgtgcgaat ctcgtccccg gtgagcgtct
     1081 cgtgctccag cagcccacgt gccaggcgat gcagttcctc gatattctcg gtcaggatcc
     1141 ggcgcgcctt cgcgtaggcc cgatcgatga tgtccttgat ctcggcatcg atctcgcgcg
     1201 ccgtcgcttc cgacacgttc ttcgcctgcg tgaccgagtg ccccaggaac acctcctggc
     1261 tgttgtcgcc gtaggcgatc atgccgagct tgtcgctcat gccccattcg gtgatcatcc
     1321 gccgcgcctg gtcggtcgcc atcttgatgt cgcccgccgc accatttgac accttatcgg
     1381 gtccgaagat gatctcctcc gccgcgcggc cacccatcgc catggtcagc tcggccagca
     1441 gcttcgattt gctcttcgag tagcggtcgc cttcgggcag cgacatcacc aggcccagtg
     1501 cccggccgcg cgggatgatc gtcgccttgt gtaccggatc gcattccggc tcatgcatcg
     1561 cacacagcgc gtggccgccc tcgtgatagg cggtcatccg cttctcggcc tcggacatca
     1621 cgagtgagcg ccgctcggtg cccatcatca ccttgtcctt ggcggcctcg aactccgcca
     1681 tcgccacaac gcgcttgccg atgcgcgcgg ccagcagcgc cgcctcgttc acaaggttcg
     1741 ccagatcggc acccgagaac ccgggtgtcc cccgcgcgat caccttcgga tcgacgtcgg
     1801 atgccagcgg caccttgcgc atatgcaccc gcagaatctt ctcgcgcccg ttcacgtcag
     1861 gattaggcac aacaacctgc cggtcgaagc ggccagggcg taacagcgcc gggtccagca
     1921 cgtcggggcg gttggttgcc gcgatcagga tgacgccctc gttgctctcg aagccgtcca
     1981 tttcaaccag catctggttc agcgtctgct cgcgttcgtc gttgccgccg cccaggccag
     2041 ccccgcgatg ccggcccaca gcatcgattt cgtcgatgaa gatgatgcag ggcgcgttct
     2101 tcttgccctg ctcgaacata tcgcgcaccc ggctagcacc gacgcccacg aacatttcga
     2161 caaagtcgga accggagatc gtgaaaaacg gcacattcgc ttcaccggcg atggcccgcg
     2221 ccagcagcgt cttgccggtg ccgggaggcc cgaccagcag aacgccctta gggatcttgc
     2281 cgcccaggcg ctggaatttc tgcgggtcct tcaggaattc gacgatctcc tgcagctcac
     2341 ccttcgcctc atcgatgccg gccacgtcat cgaacgtcac ccgcccctgc ttctcggtga
     2401 gcattcgcgc ccgcgacttg ccgaagccca tcgcgcgccc gccaccggcc tgcatctggc
     2461 gcatgaagaa cacccagacg ccaatgagca gcagcatcgg gaaccaggac agcaggtaat
     2521 gcagcagcgg attggcgccg tcatcatccg gcttggcaat tacccgcacg cccttgtcgg
     2581 tgagacgttg caccagcgtg gagtcttccg gcgtataggt ctggaacgtg cgcccgtcgg
     2641 tgagctggcc tgaaaccgtg cggccttgga tcaccacatc gcgcacacgc ccgccattca
     2701 cctcattgat gaagtcggaa taagcgacct gcgttgccgc cgtgtggttg gaacccggct
     2761 ggaacaggtt gaacagcacc accagcagca gggcgatgat gacccacaaa gccagattgc
     2821 ggccgaaatt gctcatgtct ctctgctcat atccacacgg cgcggtccaa cacggatccc
     2881 gccaacattg catgacagcc agacaccaag gaacggtgaa cggcccgcat gacgttccga
     2941 caacataggg cgtctctgcc cttcatgcat ccccaaaccg atacggcgcg cccgcggcag
     3001 ggcgggacgg ggc
//
LOCUS       DATGEM010000325        11297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1400438, whole genome shotgun sequence.
ACCESSION   DATGEM010000325 DATGEM010000000
VERSION     DATGEM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400438"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VKQ27_10410"
     CDS             complement(<1..840)
                     /locus_tag="VKQ27_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLY89386.1"
                     /translation="MADNQRWSRRPAGSTWGDFGADDERGRLNLLTPQKVLQGIAEVK
                     VGKTFCLSLPLDYPGGAILSPRRHPPVLKPTMRGDRPNMNYPLRCDDPTATDIVCDDT
                     VLLTLQYSTQWDSLAHVGQMFDVDGDGRAEDVFYNGYRAGRDIIGPAFYDAQGNMTPC
                     DEPQGARHLGVQNMAESCMQGRGVLIDLEAHFGRTGHIVSYDELMDIMRKDNVVVEPG
                     DFVLFRTGFAEMLLEMNKQPDREKLMAGTCGLDGRDPKLQQWVTDSGAVALISDNFAV
                     ESMP"
     gene            894..2486
                     /locus_tag="VKQ27_10415"
     CDS             894..2486
                     /locus_tag="VKQ27_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLY89387.1"
                     /translation="MHTLRLILLTATASAILTMPLAAADQPANPIDPSVSPPEATTNV
                     VPSAQTDNAIAQLPKLAQDILARTHIPGLAISVVRDGKTVYAAGFGVRRVGTTDAVDA
                     DTVFQIASLSKPLAATVVAHQVGAGIVSWDTVLQRFLPWFALNDPWITTHVTIGDMFA
                     HRSGLPDHAGDELEDLGYDRDQVLRRLRFAKLHSFRDEYAYTNFGLTAAAEAVAVASH
                     SDWATLSQQVLYGPLGMTSTSSRYADFIGRSNRAAGHVLINGQYAAKYQRQPDAQSPA
                     GGVSSSANDMARWMSLVLQGGMFDGKRIIAADALLPAITAQIVSSPSHVPAARAGFYG
                     YGFNVGTLASGRVSLGHSGAFALGAATNFVMIPSLGVGIDVLSNAAPIGAVEALGMDF
                     ADLVQFGVVTRDWLAAYGALMQPMSAPTGSLVGKSAPAHPAPARQPDDYAGNYHNDYF
                     GDLGVERRGGSLMLLLGPKRTPYKLSHWDGDVFTFEPIGENANAGSISKVTFAVKATG
                     RADKVDVEFFDDSGWGAFVRRQ"
     gene            complement(2487..3368)
                     /locus_tag="VKQ27_10420"
     CDS             complement(2487..3368)
                     /locus_tag="VKQ27_10420"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89388.1"
                     /translation="MAISISQITPVFAGEVSGVDITQPLSREDVAAIEAGMDRHAVLV
                     FHDQHLTDDTQRAFTRNFGSLESSPGGHIATAAERRLPLDMQDASNLDKEQKPLARDD
                     RRRMFNLGNRLWHSDSSYRAMPAKYSLLSGRVVVDRGGHTEFADMRAAYDALDDATKT
                     EIDDLVCEHSLMFSRGTLGFTELSEAERAMFRPVRQRLVRTHPVTGRKSLFLSAHCGG
                     IVGWPVPEARAFLRDLAEHATQPQFVYSHKWRQWDLVIWDNRQTMHRVTRFDETQIRD
                     MRRTTVTGDAMTVAQAA"
     gene            complement(3382..4260)
                     /locus_tag="VKQ27_10425"
     CDS             complement(3382..4260)
                     /locus_tag="VKQ27_10425"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89389.1"
                     /translation="MTVSIKPLHPLFVGEVSGTHITQPLDRDVVATIEAGMDRYAILV
                     FRDQRVTDEQQLAFSRNFGELEVTRGTGISKPGELRLDPAFADVSNLDTNNLPLARDN
                     RRRLYSLGNRLWHSDSSFKAVPATYSLLSGRIVADKGGETEFADMRAAYDALDAATRA
                     SIEDLVCEHSLQYSREQLGFDLTPEERATMRPVRQRLVRAHPVTARKSLYLASHIGTI
                     IGWPMPEARAFIRDLTEHATQPQFVYTHAWRPWDLVMWDNRQVMHRVRRYDESKPRDM
                     RRTTVAGTTVTVAQAA"
     gene            complement(4360..5250)
                     /locus_tag="VKQ27_10430"
     CDS             complement(4360..5250)
                     /locus_tag="VKQ27_10430"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89390.1"
                     /translation="MNIRQVHPVFVGEVSDIDITQPLTREQAAAIEAGMDRYAVLAFR
                     DQALTDEQQLEFSRNFGELEVTLAGQMAKPEDRRLGDRLELGDISNLTARNAIRERDD
                     RSRMYALANRLWHSDASFRAIGAGYTLLHGRVIPGKGGNTEFADMRAAYDALDDGTKA
                     EIEDLVCEHSIVFSREQIGFADYAPGNEDRLRPVRHRLVITHPVTGRKSLYLSSHIGG
                     IVGWPVPEARAFIRDLTEHATQRQFVYSHAWRVGDLVMWDNRTVMHRARRFDDLKEVR
                     DLRRTSIKGTGLEVAQAKAA"
     gene            complement(5340..6674)
                     /locus_tag="VKQ27_10435"
     CDS             complement(5340..6674)
                     /locus_tag="VKQ27_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HLY89391.1"
                     /translation="MSRLVVVSNRVAPITEGEPTAGGLAAGVLDALKQQGGIWFGWSG
                     EITDDAVSPAHMERTVGPITLYTIDLCRRDYNEYYRGFANGTLWPTLHYQISHAQFSW
                     EDFAGYRRVNAVFAQALAKVLRPDDIIWAHDYHLLGLAEALRSLGLRNRIGLFLHTPF
                     PAPGVFMTIPAHAELVRAMSQFDLLGFQTQDDRTAFTDYVERHAPAQETRTGVYPIGV
                     HVDDVRREAEAPAHQNAATRLHESLLGRPLILSVDRLDYSKGLRQRFIAVERFFEKHP
                     SHQGNVVFMQIAPPSRGDIETYREIRHALEAEAGRINGRFTEVDWMPLRYLNRGFARS
                     ALMPLYAEAQVGLVTPLRDGMNLVAKEYVAAQNPANPGVLVLSQFAGAARELDAALIV
                     NPYDEAGVATALDRALAMPRDERQERHRSMLSVMRRNSLDAWRDRFVADLAA"
     gene            complement(6872..7930)
                     /gene="pstS"
                     /locus_tag="VKQ27_10440"
     CDS             complement(6872..7930)
                     /gene="pstS"
                     /locus_tag="VKQ27_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HLY89392.1"
                     /translation="MTENLRRAIAGAALGIAFAVSAANADSITINETGSTLLYPLFKA
                     WIDGYKGVASDVVLTAASTGSGVGIDSAIGGSVRIGASDAYLSDEVAAHNPELLNIPL
                     AISAVAINYNLPGLNGANIMISGPALAAIYSGGVTEWDDPKIQALNPGVELPHKAIIP
                     VRRADASGDTFVFTQFLDFSAESWEDNPGYGTDIPWPKVAGEKTAPGNEGVIATLAAT
                     PYAIGYAGVSYASAIAKAQLGTAAVENQAGKFVLASRQAIADAAALLDPRTPPYERIS
                     LAYAPGDTSYPLVNYEYALVSQTQPNAATAGAVRSFLLWAISVTGGNGDNYLTPVGFI
                     PLPDFIRGLSQAQIAKIK"
     gene            complement(7927..9426)
                     /locus_tag="VKQ27_10445"
     CDS             complement(7927..9426)
                     /locus_tag="VKQ27_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89393.1"
                     /translation="MADGTQGAPAVVGAKTVQEYIDELPRWPDGTSLKSTPMTGMQWR
                     IWTLAAFGKFFEGFVVFMGGVTVPLINREFHLAAAQAGLVTSASLAGILVGTIALGGL
                     ADYFGRKRMFIAEMIIFCLFLALLIMVQGFVSLVICLFGVGMALGCDYPTAHMIISEN
                     IPSVSRGKLVLGAFAFQAIGAMSGIAVGAAVLMIDPELDAWRWMYATALVPAVLITVG
                     RFYITESANWLIVRGAHDEAEKAVTRLLHRTPRYPQEVALVRNVAHEASSSTKSFMAL
                     FNKDNRGATIFSSAPWFLQDLGTYGIGVFTPTMIAAALGSGPDKIRSPADLIASGIHS
                     AQAAALTTSLLIVGIIFAIALADKYGRVLLQAAGFIGCAIGLVIATLSIDAEGSMKLY
                     LIMAGVMFFNFMTNVGPNAQTYLLAGEVFPTEVRGMGAGFAAMIGKVGAVATTFGFPI
                     LLASMGTRTLLYALALTSVLGAVVTWAYRIETTGVNLDQIGRVPQEAHA"
     gene            complement(9578..>11297)
                     /locus_tag="VKQ27_10450"
     CDS             complement(9578..>11297)
                     /locus_tag="VKQ27_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89394.1"
                     /translation="YVRRHPWSHATVLVSVMVAVACAVSVQYGLKHLVDVISVGPAKA
                     QGLWLAFAILCGLVAADSLLWRVAGWAAAYTFVAVTGDIRRDLFGHLSGHSPSYFSER
                     LPGALASRITATSNAAFTIENTGSWNVVPPVVAVVCSIGFIASVNPTMAGTLVAASAA
                     LGMLIFYLAKHGTPLHRSFAAKAAAVDGELVDVVSNMSVVRAFGAMFREQRRIAGTIG
                     VELSARRSSLIYLERLRLIHAVITATLIAGIVGWGLMLWQEGQATVGDLVLITSLSIG
                     ILHCTRDLAVALVDLTQHVARLEEAIGSLLVEHELPDRPDAKQLVAGPGEVVFDHVGF
                     AYAGRAAVLRDFELTIQPGQRIGLVGSSGAGKSTVLALLQRFYDVQGGAITIDGQDIR
                     DVTQESLRAVMSIVPQDVSLFHRTVLENIRYARPDAPEEDVLAAAEMANCREFIEALP
                     DGFATMVGDRGVKLSGGQRQRLAIARALLKDTPILLLDEATSALDSESERAIQDALDR
                     LMHGRTVIAIAHRLATLKSFDRIIVMDRGRIVDDGRPAELALRPGPYRDMLRKQQLAE
                     PVQEAA"
BASE COUNT         1957 a         3452 c         3824 g         2064 t
ORIGIN      
        1 cggcatgctt tcgacggcga aattgtcaga gatgagtgcg acggcgccgc tgtcggtcac
       61 ccattgctgc agcttcgggt cgcgcccatc gaggccgcag gtgccggcca tcagcttctc
      121 gcggtccggc tgcttgttca tttccagcag catttcggcg aagccggtgc ggaacagcac
      181 gaagtcgccc ggttcgacga cgacgttgtc cttgcgcatg atgtccatca gctcgtcata
      241 gctgacgatg tgaccggtgc ggccgaaatg cgcctcgaga tcgatcagca cgccgcggcc
      301 ctgcatgcat gactcggcca tgttctgcac gccgagatgc cgtgcgccct gcggttcgtc
      361 gcagggggtc atgttgccct gggcgtcgta gaaggcgggg ccgatgatgt cgcggccggc
      421 gcggtagcca ttgtagaaca cgtcctcggc gcggccgtcg ccatcgacgt cgaacatctg
      481 gccgacatgc gcgagagagt cccactgggt ggagtattgc agcgtcagca ggaccgtgtc
      541 gtcgcagacg atgtcggtgg cggtgggatc gtcgcagcgt agcgggtagt tcatgttcgg
      601 cctgtcgccg cgcatggtgg gcttcagcac cggcggatgg cggcgcggcg agaggatggc
      661 gccgccggga taatcgagcg ggagggacag gcagaaggtc ttgccgacct tcacttcggc
      721 gatgccttgc aggacctttt gcggggtcag gaggttgagc cggccgcgtt cgtcgtcggc
      781 accgaagtcg ccccaggtgg agcctgcggg gcggcggctc cagcgttgat tgtctgccat
      841 cgacattccc ttccgttcgg cgtttgcttc gcccgcgata tagaggacgc gtcatgcata
      901 cgctgcgcct tatcctgctt acagcgacgg cgagtgcaat actaaccatg cctctggccg
      961 ctgccgacca gcctgccaac cccattgatc cgtccgtgtc gccacctgag gcaacaacga
     1021 acgtcgtgcc ctcagcacag accgacaatg cgatcgcgca gctgcccaaa ctcgcgcagg
     1081 acatactggc ccgaacgcat attccggggc tggcgatctc ggtcgtgcgc gatggcaaga
     1141 ctgtatacgc tgcggggttc ggtgttcgac gcgtaggaac gacggacgcg gtcgatgctg
     1201 atacggtgtt tcagatcgcg tcgttgtcga agccgcttgc cgcgacggtg gtggcgcatc
     1261 aggtgggggc cggcatcgtc tcgtgggaca ccgtccttca aagattcctg ccgtggttcg
     1321 cgctgaacga tccctggatc acaacgcatg tcacgatcgg tgacatgttt gcacatcgct
     1381 cgggcttgcc tgatcatgct ggcgatgagc tcgaggatct tggctatgat cgcgatcagg
     1441 tgctgcgccg gctgcggttc gcaaagctgc actcgtttcg tgacgagtat gcttatacga
     1501 actttgggct gaccgctgcc gccgaggcag ttgccgttgc gtcgcactcc gattgggcga
     1561 cgttgtcgca gcaggtcctc tatgggccgc tcggcatgac ctccaccagt tcgcgctatg
     1621 ccgacttcat cggtcggtcg aaccgtgcgg ctgggcacgt actgatcaac ggccagtatg
     1681 cggcgaaata tcagcgccag cctgatgcac aatctccggc gggcggggtg agttcatccg
     1741 ccaacgatat ggcccgatgg atgtcgctgg ttctgcaagg cggcatgttc gacggcaagc
     1801 gtatcatcgc ggcggatgcc ttgctgccgg cgatcacggc gcagatcgtg tcctcaccgt
     1861 cgcatgtgcc cgcggcgcgc gcgggcttct acggctatgg gttcaatgtt ggcaccctgg
     1921 cgtcaggccg cgtgtctctg ggtcattccg gggccttcgc attgggggca gcgacgaatt
     1981 tcgtgatgat cccctcgctc ggtgttggca tcgacgtact gagcaatgcc gcgccgattg
     2041 gcgccgttga ggcattgggt atggactttg ccgacctggt tcagttcggt gtggtcacac
     2101 gggactggct tgctgcctac ggagccctga tgcagccgat gtccgcgcca acagggtcgc
     2161 tggtcggaaa atccgcgcct gcacatccag caccggcacg acagccggac gactacgcgg
     2221 gcaactatca caatgactac tttggcgatc tcggcgtgga gcggcgcggt ggctcattga
     2281 tgctgttgct tggccccaag cgtaccccat acaagctgtc tcactgggat ggcgatgtat
     2341 tcacgttcga gcccatcggc gagaacgcca atgccggttc gatctccaaa gtcacatttg
     2401 ctgtgaaggc gactggccgg gcggacaaag tcgatgtgga gttcttcgac gattctggct
     2461 ggggcgcgtt tgtgcggcgc caatagtcac gccgcctgcg ccacggtcat cgcgtcgccg
     2521 gtcacagtgg tccgccgcat gtcacggatc tgcgtttcgt cgaaccgcgt gacgcggtgc
     2581 atggtctggc ggttgtccca tatgacaagg tcccactgcc gccatttgtg cgaatatacg
     2641 aattgtggct gcgtcgcgtg ttccgcgaga tcgcgcagga acgcacgggc ttcgggcacc
     2701 ggccagccga caatcccgcc acaatgcgcc gacaggaaaa gcgacttacg tccggttacg
     2761 gggtgcgtgc ggaccaggcg ctggcggacg gggcggaaca tggcgcgttc cgcctcgctg
     2821 agttcagtga aaccgagcgt gccgcgcgag aacatcaggg agtgttcgca gacgaggtcg
     2881 tcgatctctg tcttggtcgc atcatccagc gcatcgtagg cggcgcgcat gtcggcgaat
     2941 tccgtatgtc cgccgcgatc gacgacaacg cggcccgaga gcagcgaata ttttgccggc
     3001 atcgctcgat acgaactgtc cgagtgccac aaccggttgc cgaggttgaa catcctccgc
     3061 cgatcgtcgc gcgccagcgg cttctgctcc ttgtccaggt tggaggcatc ctgcatgtca
     3121 agcggcaggc gccgctcggc cgccgtcgcg atatggccgc cgggcgatga ctccagcgag
     3181 ccgaaattgc gcgtgaaggc gcgttgcgtg tcgtcggtca gatgctgatc gtggaacaca
     3241 agcaccgcat ggcgatccat gccagcctcg attgccgcaa cgtcctcgcg cgacagcggc
     3301 tgcgtgatgt cgacgccgga cacttcgccg gcgaagactg gcgtgatctg ggagatgctg
     3361 atcgccattg cactgtttcc ttcaggctgc ctgtgcaacc gtcaccgtgg tgccggcaac
     3421 ggttgtgcgg cgcatgtcgc ggggcttcga ctcatcgtag cggcgcacgc ggtgcatgac
     3481 ctgacggttg tcccacatca ccaggtccca cgggcgccac gcatgtgtat agacaaactg
     3541 tggctgcgtc gcgtgttcgg tcaggtcgcg gatgaaggca cgtgcctccg gcatcggcca
     3601 gccaatgatc gtgcctatgt gtgaggcgag gtacagcgac ttacgtgctg tgaccgggtg
     3661 tgcgcggaca agacgctggc gcaccgggcg catggtggcg cgttcctctg gtgtaaggtc
     3721 gaagccgagc tgctcgcgcg aatattgcag ggagtgctcg caaaccaggt cttcgatcga
     3781 tgcgcgcgtt gccgcgtcca gcgcatcgta ggcggcgcgc atgtcggcga actctgtctc
     3841 gccgcccttg tccgcgacga tgcgcccgga caggagcgaa tacgtcgcgg gcactgcctt
     3901 gaacgagctg tccgagtgcc acaggcggtt gcccaggctg tacagccggc gccggttgtc
     3961 gcgcgccagc ggcaagttgt tggtgtcgag gttggatacg tcggcaaatg caggatcgag
     4021 gcgaagttcc cctggcttgc tgatgccggt gccacgggtg acttccaact cgccgaaatt
     4081 gcggctgaag gcgagctgct gctcgtctgt caccctctgg tcgcggaaga ccaggatagc
     4141 gtaacgatcc atgccggctt caatcgtggc cacgacatcg cggtcgagcg gctgcgtgat
     4201 gtgggtgccg gagacctcgc ccacgaacag cggatgcagc ggtttgatcg atacggtcat
     4261 tgtgaacgtg cctcggagca agccagccat gacgggccag cagaatcgcg ctcgcgaggc
     4321 cagcgatgct ggccccgacg atggggattt cgtgtgtctt caggcggctt tcgcctgcgc
     4381 gacctccagc ccggtgccct tgatcgaggt gcgcctcagg tcgcgcactt ccttcaggtc
     4441 gtcgaagcgg cgcgcgcggt gcatcacggt gcgattgtcc cacatcacca gatcgccgac
     4501 gcgccaggca tgcgaataga cgaactggcg ctgtgtggcg tgttcggtca ggtcgcggat
     4561 gaaggcgcgc gcctccggca ccggccagcc gacgatgccg ccgatatgcg atgatagata
     4621 cagcgatttg cggcctgtca cgggatgggt gatcaccagg cgatggcgca cgggccgcag
     4681 ccgatcttca ttgccgggcg cgtagtcggc aaagcctatc tgctcgcggg aaaagacgat
     4741 ggagtgttcg cagaccagat cttcgatctc ggccttcgtg ccatcgtcca gcgcgtcgta
     4801 ggcggcacgc atatcggcga actcggtgtt gccgcccttg cctgggatga cgcggccgtg
     4861 cagcagcgtg tagccggcgc cgatcgcgcg gaacgatgca tcggaatgcc atagccggtt
     4921 cgccagcgcg tacatccgag acctgtcgtc gcgttcgcgt atcgcgttgc gcgcggtgag
     4981 attggagata tcgccaagtt ccagccgatc gccgaggcgc cggtcctcgg gtttcgccat
     5041 ctggccggcg agtgtcactt ccagctcgcc gaagttgcgg ctgaactcga gttgctgctc
     5101 atccgtcagc gcctgatcac gaaacgccag aacggcgtag cgatccatgc cggcctcgat
     5161 cgcggcggcc tgctcacgcg tgagaggttg cgtgatgtcg atgtcagaga cttcaccgac
     5221 gaacactgga tgcacttggc ggatgttcat tgtgggacgg ctcctggaaa tttggcccaa
     5281 gcttagcgga ggtcgcccgt tctgtcattg cgccgctggc cggatcggtc gcgggacgat
     5341 caagcagcga gatcagccac gaagcggtcg cgccaggcgt ccaggctgtt gcgccgcatc
     5401 accgacagca tcgacctgtg ccgctcctgg cgttcgtcgc ggggcatggc gagggcacga
     5461 tcaagcgctg tcgcgacgcc ggcttcgtcg taaggattga cgatcagcgc ggcgtccagt
     5521 tcgcgcgcgg cgccggcgaa ttgcgagagg accagcacgc caggattggc tgggttctgt
     5581 gctgcgacgt attcctttgc taccaggttc atgccgtcgc gcaggggagt cacgaggccc
     5641 acctgggcct cggcgtagag cggcatcagt gcgctgcggg cgaagccgcg gttgaggtag
     5701 cggagcggca tccagtccac ctcggtgaag cggccgttga tccggccggc ctcggcttcc
     5761 agcgcgtggc ggatttcgcg ataggtttcg atgtcgccgc gcgatggcgg ggcgatctgc
     5821 atgaacacca cattgccctg gtggctgggg tgcttctcga agaagcgctc caccgcgatg
     5881 aagcgctgac ggaggccctt ggagtaatcc agccggtcga cactgaggat caacggtcgt
     5941 ccaagcaggc tttcatgcag ccgggttgcg gcgttctgat gtgccggcgc ctccgcttcg
     6001 cggcggacgt cgtcgacatg cacgccaatc ggatacacgc ccgtcctggt ctcctgcgcc
     6061 ggcgcgtggc gctccacgta atcggtgaat gcggtcctgt cgtcttgcgt ctggaagccg
     6121 agcagatcga actgcgacat ggcgcggacc agctcggcgt gcgccgggat cgtcatgaag
     6181 accccaggtg ccgggaatgg cgtgtgcagg aagaggccga tgcgattgcg cagtccgagt
     6241 gaccgcagtg cttcggccag accgagcagg tgataatcgt gtgcccagat gatgtcgtct
     6301 ggccgcaaca cctttgccag cgcctgcgca aacacagcgt tcacgcggcg atagccggcg
     6361 aaatcctccc aggaaaactg cgcgtggctg atctgatagt gcagggtggg ccatagcgtg
     6421 ccgttggcga acccgcggta gtattcgttg tagtcgcgcc ggcagaggtc gatcgtatac
     6481 agcgtgatcg gaccgaccgt gcgctccatg tgtgccggcg agacggcatc gtcggtgatc
     6541 tcgccgctcc agccgaacca gatgccaccc tgctgcttga gcgcatccaa cacgccggcg
     6601 gcgaggccac cagcggtggg ttcgccttcc gtgattggcg ccactcggtt cgagacgaca
     6661 acaagccgcg acatccctca ggaccctacc ttcgggaaaa ccagatcaca tgactgtgtc
     6721 cgatacgtgg cggatttgca ccgaatttac tgctggatcc gacgggaaag ggcgtggatg
     6781 ccggggcttt gcccggcatg gcgagaatcg ccggtcggtc ccgctcaatc atgtctggtg
     6841 gcgcgcgttg ccgcgttgcc gggtgcgaat atcacttgat cttggcgatc tgcgcctggc
     6901 tcagtccgcg gatgaaatcg ggcagcggga tgaagccgac gggtgtcagg tagttgtcgc
     6961 cattgccgcc cgtgaccgaa atcgcccaca gcagaaatga ccgcaccgct ccggccgtag
     7021 cggcgtttgg ctgcgtttgc gagaccaggg cgtattcgta gttgaccaat gggtagctgg
     7081 tgtcgcccgg ggcatacgcg aggctgatgc gctcgtaggg tggcgtgcgt gggtcgagca
     7141 gagcggcggc atcggcgatc gcctgccggg aggcgaggac gaacttgccg gcctggttct
     7201 cgactgcggc cgtgccgagc tgggctttgg caatcgcgct ggcgtaactg acgcccgcat
     7261 agccgatcgc gtagggcgtg gctgcgagcg tcgcgatgac gccttcgttg ccgggtgcgg
     7321 ttttctcccc ggcgaccttg ggccaaggga tgtcggtgcc ataacccgga ttgtcttccc
     7381 agctctcggc cgagaaatcc aggaattggg tgaatacgaa cgtatcgcca gacgcatcgg
     7441 cgcggcggac cggaatgatg gccttgtgcg gcagttccac gcccggattc agcgcctgga
     7501 ttttgggatc atcccactcg gtcacgccgc cggaatagat ggcggcgagc gccgggccac
     7561 tgatcatgat gttcgcgcca ttgaggcccg gcagattgta gttgatggcg accgcggaga
     7621 tggcgagcgg tatgttgagg agctccgggt tatgcgctgc gacctcgtcg gagagatagg
     7681 catcggaggc gccgatacga acggagccgc cgatcgctga gtcgataccg acgccggaac
     7741 cggtgctcgc cgccgtcagt acgacgtcgg acgcgacgcc cttgtagccg tcgatccagg
     7801 ccttgaagag cgggtacagg agagttgagc cggtctcgtt gatggtgatg ctgtcggcgt
     7861 tggcggcaga gaccgcgaag gcgatgccaa gggcggcccc ggcgatcgcg cggcgaaggt
     7921 tctctgtcat gcgtgcgcct cctggggaac gcgcccgatc tggtcgagat tcacgccggt
     7981 cgtctcgatg cggtaggccc aggtcacgac cgcgcccaac accgaggtga gcgcgagcgc
     8041 gtaaagcagc gtacgggttc ccatggaggc cagcaggatc gggaagccga aggtggtggc
     8101 gaccgcgccc actttgccga tcatcgccgc gaagcccgcg cccatgccgc gtacctcggt
     8161 cgggaacact tcgcccgcga gcaggtaggt ctgtgcgttg gggccgacat tggtcatgaa
     8221 gttgaagaac atcacgccgg ccatgatcag gtagagcttc atcgagccct cggcatcgat
     8281 cgacagcgtg gcgatgacaa gcccgatggc acagccgatg aagccggcgg cctgcaggag
     8341 gacgcgtcca tatttgtcgg cgagcgcgat tgcgaagatg atgccgacga tcagcaacga
     8401 ggtcgtcagc gccgcggctt gcgcggaatg gatgccgctt gcgatcagat cggcgggact
     8461 gcggatctta tcgggaccgc tgccgagggc ggccgcgatc atggtggggg tgaagacgcc
     8521 gatgccgtag gtaccgagat cctggaggaa ccagggcgcc gacgagaaga tggtggcgcc
     8581 gcggttgtcc ttgttgaaca gcgccatgaa ggactttgtt gatgagcttg cctcgtgcgc
     8641 gacgttgcgg acgagagcca cctcttgcgg gtagcgcggc gtacggtgga ggaggcgggt
     8701 aacggctttc tccgcctcat cgtgagcgcc acgcacgatg agccagttgg cgctttcggt
     8761 gatgtagaag cggccgacgg tgatgagtac ggccggcacc agtgcggttg cgtacatcca
     8821 tcgccaggca tcgagctccg ggtcgatcat aagcacggcg gcgcctactg cgatgccgga
     8881 catggcaccg atcgcctgga aggcgaaggc gcccagcacc agcttgccgc gcgagacgct
     8941 ggggatgttc tccgagatga tcatgtgcgc ggtcggatag tcgcagccga gcgccatgcc
     9001 gacgccgaac aggcagatca ccagcgacac gaagccctgg accatgatca gcagggcgag
     9061 gaacaggcag aagatgatca tctcggcgat gaacatgcgc ttgcggccga aatagtccgc
     9121 gagaccgccg agtgcgatgg tgccgacaag gatgcccgca aggcttgccg aggtgacgag
     9181 gcccgcctgg gcggcggcaa gatggaattc ccggttgatc agcggcaccg tgacgccgcc
     9241 catgaagacg acgaacccct cgaagaactt gccgaacgcc gcgagcgtcc agatgcgcca
     9301 ctgcatgccc gtcatgggcg tggatttcag cgaggtgccg tcgggccaac gcggcagttc
     9361 gtcgatgtat tcctgaactg tctttgcgcc tacgactgcc ggcgcgccct gtgtaccatc
     9421 ggccattggc ggctcaatcc cactcgtccc cacggcgcga ccgccatcac ctggccttgg
     9481 cggccaggtg gcggtgcgac agtgatcagg gtggccaaaa actggcgcga cggcacgtga
     9541 tgctttgatg tcagccgccg ggcaggccgg cagcggctta cgctgcctcc tgcaccggtt
     9601 cggcaagctg ctgcttgcgc agcatgtcgc gataggggcc ggggcgcagc gcgagctccg
     9661 ccggccggcc gtcatcgacg atgcggccgc gatccatgac gatgatccga tcaaagctct
     9721 tcagcgtcgc caggcggtgt gcgatggcga tcaccgtgcg cccatgcatc agccggtcga
     9781 gtgcgtcctg gattgcccgt tcgctctcgc tgtccaatgc actggtcgcc tcgtcgagca
     9841 gcaggatcgg cgtgtccttg agcaatgccc gggcgatggc cagccgctga cgctgtccgc
     9901 cggagagctt cacgccccga tcaccgacca tggtggcgaa gccatcggga agcgcctcga
     9961 tgaactcgcg gcagttcgcc atctcggcgg cggccagcac gtcttcctcg ggcgcatcgg
    10021 gacgggcgta acggatgttc tccagcaccg tacggtggaa caggcttacg tcctgcggga
    10081 caatcgacat cacggcgcgc aggctttcct gggtcacgtc gcggatgtcc tggccgtcga
    10141 tcgtgatggc gccgccctgc acatcgtaga accgctgcag aagcgccagc acggtggact
    10201 tgccggcgcc ggaggagccg accagcccta tgcgctgccc aggctggatg gtcagttcga
    10261 agtcgcgcag cactgcggct cgacccgcat aggcgaaacc gacatggtcg aacaccactt
    10321 cgcctgggcc ggcgaccaat tgcttcgcgt cgggacgatc cggcagttcg tgttcgacca
    10381 gcagcgagcc gatcgcttcc tccagccgcg caacgtgctg ggtcaggtca accagggcca
    10441 ccgcgagatc gcgcgtgcaa tgcaggatgc cgatggagag agacgtgatc agcaccaggt
    10501 cgccgactgt cgcctgacct tcctgccaga gcatcagccc ccagccgacg atgccggcga
    10561 tcagcgtcgc ggtgatgacc gcgtggatca gccgcagccg ctcgagatag atcaggctgg
    10621 agcgacgagc gctcaactcg acgccaatcg tgccggcgat gcgccgctgc tcacggaaca
    10681 tcgcgccgaa cgcgcgcacc acggacatgt tgctgaccac atcgaccagc tcgccatcca
    10741 ctgcggcggc cttggcggcg aaggagcgat gcagcggcgt gccgtgcttg gccaggtaga
    10801 agatcagcat tccgagcgct gccgaggcgg caaccagcgt gccggccatc gtcgggttca
    10861 ccgaggcgat gaagccgatg gagcagacca ctgccacgac cggcggcacc acgttccagg
    10921 atccggtgtt ctcgatggtg aaggcggcat tggaggtcgc ggtgatgcgg ctggcgagcg
    10981 cgccaggcag gcgctcggag aagtagctcg gcgaatggcc agagaggtgg ccgaacaggt
    11041 cgcgccgtat atcgccggtc acggccacaa atgtataggc ggccgcccag cccgcgacac
    11101 gccatagcag gctgtcggca gcgaccagtc cgcatagtat ggcaaatgcc aaccacaggc
    11161 cctgggcctt cgccggaccg acggagatca catcgaccaa atgcttcagg ccgtactgaa
    11221 cggagaccgc gcaggccacc gccaccatca ccgatacaag tactgtggcg tggctccagg
    11281 gatggcgccg cacgtaa
//
LOCUS       DATGEM010000326         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_916971, whole genome shotgun sequence.
ACCESSION   DATGEM010000326 DATGEM010000000
VERSION     DATGEM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916971"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..179
                     /locus_tag="VKQ27_10455"
     CDS             51..179
                     /locus_tag="VKQ27_10455"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89395.1"
                     /translation="MLAASLGLSRMYRNDLAQLDAAMVLYDAFYRWARDAVNETHS"
     gene            143..1819
                     /locus_tag="VKQ27_10460"
     CDS             143..1819
                     /locus_tag="VKQ27_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLY89396.1"
                     /translation="MGAGRCKRDPLMMSSRKTILGLFGVVALGAAALYEFALPGFSSA
                     RSEPPKAEIAVATWMLRHSVPADAAQQRNPLGADPADIAAGHDLFQKNCEICHGYDGS
                     GKTQIGGGEYPRAPVLMTLLPSLSDGEVFYHVRNGIRLTGMPAWGFPDREMWQLVLYM
                     RHLPKTAPLPVEATADASDGADKHFVGSESCKTCHAEIYERWKKTPMANVVRDPHEHP
                     DAFIPDFSKPDPLLTFSKDDVAFIYGSIWKQRYFKKVGDDYVPLPAQWDVTHKKWAPY
                     FVKNGTDWWSTLFPPDNNQRPTSQLCDGCHSVDYDIATHKVAEWNVGCEACHGPASQH
                     VKNPIRETVINPARLDFVKANDTCIQCHSQGRPPGNPIDGKLYDWPVGFHMGANLSDH
                     WRLEEHKLGETTFTHFPDGTAHKNRMQGNDFVQSLMYTRGVTCFSCHDVHGTANPAQL
                     RLPPSQICLSCHGPNTQNGPHTASIEAHTHHKADSPGSQCVACHMPKIEQTIADVNVH
                     AHTFRVVMPSETDAYKIPNSCNGCHADKSTEWASTALKSWKTQSPWRMDK"
     gene            complement(1861..2439)
                     /locus_tag="VKQ27_10465"
     CDS             complement(1861..2439)
                     /locus_tag="VKQ27_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HLY89397.1"
                     /translation="MFDSFLVSTGVVALAEVGDKTQLLSLLLAAKYRKPVPIILGVFA
                     ATLLSHAGAGGIGALLADFLRPAILNWAVVASFALMAGWILVPDKLDDAESGISKGHS
                     GIFVTVALTFFMAEMGDKTQIATVALAAQFHAFFSVAGGTTLGMMIANVPVVFLGYRF
                     ADRLPAKAVRGVAALIFVVLGGLALRNALMTP"
     regulatory      complement(2447..2613)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            complement(2780..3526)
                     /locus_tag="VKQ27_10470"
     CDS             complement(2780..3526)
                     /locus_tag="VKQ27_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89398.1"
                     /translation="MGVAVALTALGVAARADELRLISPNEIDYREIEIEHEGSAGFDR
                     SPDKDGGRVYSLDLGTGVTRWWHVGVELDWEREAGQGNPVQLDGAQIESLVRLTEPGE
                     NWLDAGVYAEYSRSSLHGRDSPDDLLVGGVFLKDIGRTTHTLNLFFDRAFSPDEDVSG
                     VRFSYAWQSRWNLWRQFAPAIEAFGRAGRVDRPDGFQDGQLIVGPVATGTFLLGPAGR
                     LRYEAGYLLGATEASPSGTVRWKIEMEIPF"
     gene            complement(3538..>3975)
                     /locus_tag="VKQ27_10475"
     CDS             complement(3538..>3975)
                     /locus_tag="VKQ27_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HLY89399.1"
                     /translation="SPRSGRPMLAAYARLVPHHRPAVRFNLTFGSAVALRHYAIMRLA
                     ASFLLLVVAAAPLAADEPLRLVLHEHKFTPERLDVPAHAKFRLQVRNDSDATIEWESD
                     ELDREEVILPGEERSIFLGPLEPGTYPFYDDRYQASKGALVAK"
BASE COUNT          794 a         1291 c         1212 g          678 t
ORIGIN      
        1 tccggggcgc cgatacggcg aggttcgaca ttgcgcccga ggcttcgggt ttgctggccg
       61 cctcgctcgg cctgtcgcga atgtatcgga acgacctcgc ccaattggac gcggcaatgg
      121 tcctctatga cgccttttat cgttgggcgc gggacgctgt aaacgagacc cactcatgat
      181 gtcatcccgt aagacaatcc tcggcctttt cggtgtcgtc gcgctcggag cggcggctct
      241 ctatgaattc gcgcttcccg gcttctccag cgcccgttcc gagcctccga aagcggagat
      301 tgccgtcgcc acctggatgc tgcgtcacag cgttcccgcc gacgcggccc aacagcgtaa
      361 tcctctgggg gccgatccag ccgacatcgc ggccgggcat gatctgttcc agaagaattg
      421 cgagatctgc cacggttatg acggtagcgg gaaaacgcag atcggcggcg gggaataccc
      481 gcgcgcgccc gtcttgatga cgttgctgcc ctcgctatcc gacggcgagg tcttctacca
      541 tgtccgcaac ggcattcggc tgaccggtat gccggcctgg ggtttccccg accgcgagat
      601 gtggcaactc gtcctctata tgcgccatct gccgaaaacg gcgcctctgc ccgtcgaagc
      661 gaccgccgat gcgtcggacg gagccgacaa gcattttgtc ggatcggaaa gctgcaaaac
      721 ctgccatgcc gagatctatg agcgttggaa aaagacgcct atggcgaatg tcgtgcgcga
      781 tccgcatgag catcccgacg ccttcattcc cgacttctcg aaaccagacc cgctcctgac
      841 cttctcgaaa gacgatgtcg ccttcatcta tggcagcatc tggaagcagc gctatttcaa
      901 gaaggtcggc gacgactatg ttccgctacc ggctcaatgg gacgtcactc acaaaaaatg
      961 ggcgccttat ttcgtcaaga acggcaccga ctggtggtcg acgctgttcc cgccggacaa
     1021 taatcagcgg ccgaccagcc agctctgcga cggctgccat tcggtcgatt acgacattgc
     1081 gactcataag gtggccgagt ggaatgtcgg ctgtgaagct tgtcacggcc cggcaagcca
     1141 gcatgttaaa aacccgatcc gcgagacggt catcaacccg gcccggctcg acttcgtgaa
     1201 ggcgaacgat acctgtatcc agtgccattc acagggccgc ccgcccggca atccgatcga
     1261 cggcaagctc tatgactggc ccgtcggctt tcatatgggc gccaatctca gcgaccattg
     1321 gcggctggaa gagcacaaac tgggcgagac gaccttcacc cattttccgg atggcacggc
     1381 gcataagaac cgcatgcaag gcaacgattt cgtgcaaagc ctgatgtata cgcgcggcgt
     1441 cacctgcttc agctgccacg atgtccatgg caccgcgaat ccggcgcagc tccgtctgcc
     1501 tccatcgcag atctgcctgt cctgtcacgg tccgaatacc cagaacggac cgcacaccgc
     1561 gtccatcgag gcgcataccc atcacaaggc ggacagcccc ggcagccagt gcgtcgcctg
     1621 ccacatgccg aagatcgagc agacgatcgc cgatgtgaat gtgcacgccc atacattccg
     1681 cgtggtgatg ccgtccgaaa cggacgccta taagatcccc aattcctgca atggctgcca
     1741 cgcggacaaa tcgacagaat gggcgagcac ggcgctcaag agctggaaaa ctcagtcgcc
     1801 ttggcggatg gataaatagc ggcggcggca taggggaaat gccgccgcgt ccatccgctg
     1861 ctacggcgtc atcagcgcgt tccgcagcgc caatccgccc agcacgacga agatcagcgc
     1921 cgccacccca cggacggcct tggccggcag ccggtcggcg aagcggtagc cgaggaagac
     1981 gaccgggaca ttggcgatca tcatgcccag cgtggttccc cccgccaccg agaagaacgc
     2041 atgaaactgg gcggccaggg cgactgttgc gatctgcgtc ttatcgccca tctcggccat
     2101 gaagaaggtc agcgcgacgg tcacgaagat gccggaatgc cctttcgaga ttccgctctc
     2161 ggcgtcgtcg agcttgtcgg gcaccaggat ccaaccggcc atcagggcga aggacgcgac
     2221 caccgcccag ttcaggatgg cggggcgcag gaagtccgcc agcagtgcgc cgatgccacc
     2281 cgcgccggcg tggctcagca gggtcgcggc gaacacaccc aggatgatcg gaaccggctt
     2341 gcggtatttc gccgccagca acagcgacag cagctgcgtc ttgtcgccga cttcggcgag
     2401 ggcgacgaca ccggtggaaa ccaggaagga gtcgaacatc gagacaagtg ctccaggccg
     2461 gacacgacat tgaccgcgtc gcgtccccgg ccccggagac aacgcggtca atggtctcgc
     2521 caaatcctgg atcgcccgca ccatgcctcg cggcaagttt gttgatacgg gcccctccgg
     2581 agtggagggc gactactccc caatgacggg cggcaatcta atcggcccgc tcgacggggg
     2641 ccagtgaaaa ttttgctagg gcgggagacg ttagactcgg ccagaagact catcccgctc
     2701 ggaggcgcgt tgcgacgacc ccgagggcgc gggataggct accccgccat agcacgatga
     2761 cgacgaccgt catcgtgttt taaaacggga tctccatctc gatcttccag cgcacggtgc
     2821 ccgacggact ggcttcggtg gcgccgagca gatagccggc ctcgtagcgc agccttcccg
     2881 ccggtcccag caggaaggtg ccggtcgcca ccgggccgac gatcaactgc ccatcctgga
     2941 aaccgtcagg ccgatcgacc cggccggcgc ggccgaacgc ctcgatggcg ggcgcgaatt
     3001 gccgccaaag attccatcgg ctttgccagg cataggagaa gcgaaccccg ctgacatcct
     3061 cgtcggggct gaaggcccga tcgaaaaaca ggttcaatgt atgggtggtc cggccgatat
     3121 ccttgagaaa cacgccgcct accagcaggt cgtcgggtga gtcccggccg tgcagggagg
     3181 atcgggaata ttcggcatag actcccgcat cgagccagtt ctcgccgggc tcggtcaagc
     3241 gaaccaggga ttcgatctgc gcgccgtcca gttgaaccgg gttgccctga cccgcctcgc
     3301 gctcccagtc gagctcgacg ccgacatgcc accagcgcgt gacgcccgtc cctagatcca
     3361 gagaatagac ccgcccgcca tccttgtccg gcgagcggtc gaagcctgcc gagccctcat
     3421 gctcgatctc gatctcgcgg taatcgatct cgttgggcga aatcaggcgc agctcatccg
     3481 cgcgcgcggc aacacccagg gccgtcagcg caaccgcgac tcccatcctc gccgatctca
     3541 tttggcgacc agcgcgcctt tgctggcttg gtagcggtcg tcatagaagg ggtaagtacc
     3601 gggctcgagc gggccgagaa agatcgaccg ctcctcgccc ggcaagatca cctcttcgcg
     3661 gtcgagttcg tcgctctccc actcgatggt cgcgtcgctg tcgttcctga cctgcaggcg
     3721 gaatttggca tgggccggca cgtcgagccg ttcgggcgtg aatttgtgct cgtgaagcac
     3781 gaggcgcagc ggctcgtcgg cggccaaggg tgcggccgcc acgacaagca gaagaaaaga
     3841 agcggcaagt cgcatgatgg catagtgtcg cagagcgacc gcggaaccga aagtcaggtt
     3901 aaacctgacg gccgggcggt ggtgaggcac caatcgggcg tatgcagcca gcattggccg
     3961 tcctgatcgt ggcga
//
LOCUS       DATGEM010000327         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1399942, whole genome shotgun sequence.
ACCESSION   DATGEM010000327 DATGEM010000000
VERSION     DATGEM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399942"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2787
                     /locus_tag="VKQ27_10480"
     CDS             <1..2787
                     /locus_tag="VKQ27_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HLY89400.1"
                     /translation="FNADFDGDQMAVHVPLSLEAQLEGRVLMMSTNNILSPANGKPII
                     VPSQDIVLGLYYLSLDTPEFREAADADVPAFGTIGEIEFALHAKAITLHSKIKARYET
                     IDAGGNPIRQIVVTTPGRMMIAQILPKHPMVPFELINRQLTKKSVSDVIDMVYRHCGQ
                     KECVIFADRLMGLGFGQAAKAGISFGKDDLIIPVEKDELIHRTQAEVKEFEQQYQDGL
                     ITAGERYNKVVDAWSRCTDEVAGAMMKEISKQEVGKQTNSVWMMSHSGARGSPAQMRQ
                     LAGMRGLMAKPSGEIIEQPIIANFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDCIIFEEDCGTERGLTVRAVMDGGEVVSALSERILGRTSAEDVTDPVSNE
                     VIVPANTLIEEDAADAIEKAGVEAVKIRSVLTCESTVGVCGHCYGRDLARGTPVNIGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAAQRGAEQSSVEASHEGIVNVKNRNVVMNSQG
                     VPVVMSRNCEIVLTDDKQRERARFRVPYGARLLVDEGQQVARAQKLAEWDPYTLPIIT
                     ERNGKVEYIDLIEGITLVERMDEVTGLTSKVVVDYKQGAKGVDLRPRLQLKDDHGEVV
                     KLPNGTDARYFLAPDSILSVDAGAVVAAGDVLARIPREGSKTRDITGGLPRVAELFEA
                     RRPKDHAVIAENDGRVEFGKDYKNKRRVIVKNDETTEETEYLIPKGKHVSVQEGDFVR
                     RGDPLVDGPRVPHDILKVMGVEALSDYLVNEIQDVYRLQGVKINDKHIEVIVRQMLQK
                     VEILDPGDTTFLAGEQVDRTEFDAVNSKLGKDEKPAHAMPVLQGITKASLQTNSFISA
                     ASFQETTRVLTEAATAGKTDTLTGLKENVIVGRLIPAGTGSVMNRLRAIAAGRDQRTL
                     SMGTTPQITEEAAE"
     gene            3233..3682
                     /locus_tag="VKQ27_10485"
     CDS             3233..3682
                     /locus_tag="VKQ27_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89401.1"
                     /translation="MDDQPSAATGFHHVAIRAVDFDATIRFYTEGLGCSVRYRFSVPG
                     RIDRAAFLDMGDGRFIEVFGSGSTVQSEGRRRRPDEEPTEGALLHFCFRVADVDTAYA
                     RALAAGAVSRIAPRERQLGGEPRAEVQIAFITGPNGEVIEFLKSDLL"
BASE COUNT          751 a         1113 c         1334 g          691 t
ORIGIN      
        1 ttcaacgccg acttcgacgg cgaccagatg gcggtgcacg tgccgctgtc gctggaagcg
       61 cagctcgaag gccgcgtgct gatgatgtcg accaacaaca ttctcagccc cgctaacggc
      121 aagccgatca tcgtgccgtc gcaggacatc gtgcttgggc tgtactacct gtcgctcgac
      181 acgcctgagt tccgggaagc agcggatgct gacgtgccgg cattcggcac gatcggtgag
      241 atcgagttcg cgctgcacgc caaggcgatt acgctgcaca gcaagatcaa ggcgcgctac
      301 gagacgatcg acgcgggtgg caatccgatc cgccagatcg tggtgaccac gccgggccgg
      361 atgatgatcg cgcagatcct gccgaagcac ccgatggtgc cgttcgagct gatcaaccgg
      421 cagctcacca agaagagcgt ctccgacgtg atcgacatgg tgtatcgcca ttgcggtcag
      481 aaagagtgcg tgatcttcgc cgaccggctg atggggcttg gctttggtca ggcggcgaag
      541 gcgggcatct ccttcggcaa ggatgacctg atcatcccgg tcgagaagga tgagctgatc
      601 caccgtaccc aggccgaggt gaaggagttc gagcagcagt atcaggacgg cctgatcact
      661 gctggcgagc gctacaacaa ggtggtggac gcctggtcgc gctgcaccga cgaagtcgcc
      721 ggcgcgatga tgaaggagat ctcgaagcag gaggtcggca agcagacaaa ctcggtgtgg
      781 atgatgagcc attcgggtgc gcgtggttcg ccggcgcaga tgcgtcagct tgccggtatg
      841 cgcgggctga tggcgaagcc gtcgggcgag atcatcgagc agccgatcat cgccaacttc
      901 aaggaaggcc tgtcggtgct ggagtacttc aactccacgc acggcgctcg caagggcttg
      961 gccgacaccg cgttgaagac ggcgaactcc ggctacctga cacgccgtct ggtggacgtg
     1021 gcgcaggact gcatcatctt cgaagaggac tgcggcaccg agcgcggctt gaccgtgcgc
     1081 gcggtgatgg atggtggcga ggttgtctcg gcactgtctg agcgcatcct gggccgcacc
     1141 tcggctgagg acgtgaccga ccctgtctcc aacgaggtga tcgtgccggc caacacgcta
     1201 atcgaggaag atgccgccga tgcgatcgaa aaggccggtg tcgaggcggt taagatccgc
     1261 tcggtgctga cctgcgagtc gactgttggc gtgtgcggcc attgctacgg gcgcgatctg
     1321 gcccgcggca cgccggtgaa tatcggcgag gccgtcggcg tcatcgcggc gcagtcgatc
     1381 ggcgaacccg gcacgcagtt gacgatgcgc acgttccata tcggcggcgc ggcgcagcgt
     1441 ggtgccgaac agtcgagcgt tgaggcgagc cacgaaggca tcgtcaacgt caagaaccgc
     1501 aacgtggtga tgaacagcca gggcgtaccg gtggtgatgt cgcgcaattg cgaaatcgtc
     1561 ctgacggacg acaagcagcg cgagcgtgcg cgcttccgtg tgccgtatgg cgcgcggttg
     1621 ctggtggatg aaggtcagca ggtggcgcgc gcacagaagc tggcggagtg ggatccgtac
     1681 actttgccga tcatcaccga gcgcaacggc aaggtcgaat acatcgacct gatcgaaggc
     1741 atcacgctgg tcgagcgtat ggacgaggtc acgggtctga cgtcgaaggt ggtggtggac
     1801 tacaagcagg gcgccaaggg cgtcgatctg cggccacgcc tgcagctgaa ggacgaccac
     1861 ggcgaggtcg tgaagctgcc gaacggcact gatgcgcgct acttcctcgc acctgactcc
     1921 atcctttcgg tggatgccgg tgccgtggtg gcggcgggcg acgtgctggc gcgtatccca
     1981 cgtgaaggca gcaagacgcg cgacatcacc ggtggtctgc cgcgtgtggc ggaattgttc
     2041 gaggcacgcc ggccgaagga tcatgctgtc atcgccgaga acgatggccg cgtggagttc
     2101 ggcaaggact acaagaacaa gcgccgcgtc atcgtgaaga acgatgagac gacggaagag
     2161 accgagtacc tgatcccgaa gggcaagcac gtctcggtgc aggaaggcga cttcgtgcgc
     2221 cgtggcgatc cgctggtcga cgggcctcgg gtgccgcacg acatccttaa ggtgatgggt
     2281 gtcgaggctc tgtcggacta cctagtgaac gaaatccagg acgtgtaccg cctgcagggt
     2341 gtgaagatca acgacaagca catcgaggtt atcgttcgcc agatgctgca gaaggttgag
     2401 attctcgatc cgggcgatac gacgttcctg gccggcgagc aggtggaccg caccgagttc
     2461 gatgctgtga acagcaagct cggcaaggac gagaagccgg cgcatgcgat gccggtgctg
     2521 cagggcatca ccaaggcgtc gctgcagacc aacagcttca tcagtgccgc ttcgttccag
     2581 gagacgacgc gcgtgctgac cgaggctgcg acggcaggca agaccgacac gctgaccggt
     2641 ctgaaggaga acgtgattgt cggacgcttg atcccagcag gcacgggctc ggtcatgaac
     2701 cggctgcgcg cgattgctgc agggcgcgat cagcggacgc tgagcatggg tacgacgccg
     2761 cagattaccg aggaagcggc ggagtaagca ttcagcccgc gagggttggt gggccggcgg
     2821 cgcgatggtg ccgccggcct tttctttttg ggggagagag ccgacgacag gcctgctcag
     2881 ccgctgataa tcgcttgtgc agtaggaggc tgggcggacc gcgcgccgcg acgaaccaag
     2941 aacgatcact atcgcctctg atgccgagtt attcgttcga gcagagcagg cagggattga
     3001 cgttggcgcc agcgctaatg cgttctttcg cggccttgtc catgcgtctc acgtcggcag
     3061 gcggcggcag gacgcgggaa gtccaaaacg tgctccccat gatggatcct ttgtcgccag
     3121 tcagagcttg ccctgagatt gcggagtggc cgaatgtcgg gttggctgat ctggcttggc
     3181 gccaaggtcg ggcgactagc acccgtacta caaagacagg aggatcgaac caatggacga
     3241 ccagccttcg gccgcgacgg gcttccacca tgttgcgatc cgcgctgtgg attttgacgc
     3301 gacgatccgt ttctacaccg aaggccttgg ctgctccgtc cgctaccgct tctcagtgcc
     3361 cgggcggatc gatcgcgccg cgtttctcga tatgggcgac ggccgtttca ttgaggtgtt
     3421 cgggtcgggt tccacagttc aatcggaggg ccgccgccgc cgccccgacg aggaaccgac
     3481 ggaaggagcg ctgctgcatt tctgcttccg cgttgcggac gtggatacgg cctatgcgcg
     3541 agcgctggcg gcaggcgcag tgtcgcgtat cgcgccaaga gagaggcagc ttggtggtga
     3601 gccgcgggcc gaggttcaga tcgccttcat tactggtccg aatggtgaag tgatcgagtt
     3661 cctgaagagc gatctgctct gagcagccct gaggtttcac caacctgctg cgtcttccag
     3721 ctgccgaact gatcgaacgg agcattcgca atacgctggt ccgtgccgga tgtacccagc
     3781 gtcagtaacg cgctctcatt gcctggacct catgtcaccg ggcttccgca acggtaacca
     3841 gcagttgcgt tggctggggt tgaccagtca cggcatgacc ggggtagtc
//
LOCUS       DATGEM010000328        11286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_938211, whole genome shotgun sequence.
ACCESSION   DATGEM010000328 DATGEM010000000
VERSION     DATGEM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938211"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1087
                     /locus_tag="VKQ27_10490"
     CDS             68..1087
                     /locus_tag="VKQ27_10490"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY89402.1"
                     /translation="MQRGKLSRRRVLTGAAGVSAATILHWPANAAEFSYKLGSSSPME
                     HPAMAAQQDAANKIKQESNGRLEITIYPNSVLGGDTAMIAQTISGAMEMYNLPIDLLA
                     PRNPVCGLPGVGFAFPDYAHVWAAMDGEFGNFLRAAAEEIGLYCLEKGYDHGFRQITT
                     RTKPITSPDDLKGFKIRLPVAPYLISLFRHLGASPTAINFNEVYSALQTGIVDGQENP
                     LVLIDTAKLFEVQKYCSLTNHVWAGFHTSFNVAAWKKLPPDLQEMCHHNFSEVALKER
                     DAFVTMTKSEQQNLSGKGLTFNSPDTKPFRDVLAKTGFYPDMKKTAGDKGWALLEKYV
                     GPLTS"
     gene            1084..2961
                     /locus_tag="VKQ27_10495"
     CDS             1084..2961
                     /locus_tag="VKQ27_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLY89403.1"
                     /translation="MSLDATTVPQDVPGWAVLRRCAFLIDQGIGTACEAIGAALVLAE
                     VCILFAGVVSRYALDSPLFWTDELANFLFLWLSMLGMVVALRRDGHMRLTTVANWVPP
                     HIARWFAAVASLVVIAFVLEVLWPATQYLDQQRYVELITLQISDGYRVTAIVVGMALA
                     AVISLLRLLETTTWRSFCLAVLTVGGIAALLWAGRPVLLSMGFGSLIVFFVVIVAACV
                     AIGVPIGFGFGIATMSYLGLTSALPLSVVVNRMDEGMSNLLLLSVPMFVFLGLLMEMA
                     GIARVMVAFLAALVGHVRGGLSYVLLGAMYLVSGISGSKAADMAAVAPVLFPEMQRRG
                     SHPGELAALLASSSAMSETIPPSLVLITIGSVANVSITALFTGGLLPAAVAAVALLVM
                     SWYRSRHMDISGGERASTGQILRTFVIAVPGLTLPFVIRAAVIDGVATATEVSTVGVV
                     YTAVLGMFVYRQFDWRRIYPILVDTLSLTGAVMLIIGTATAMAWALTQSGFSQWLVAT
                     MATVPGGKLGFLAITAVTFVILGSVLEGIPAIVLFGPLLFPVAKAMGVHEVHYAMIII
                     LAMGIGLFAPPFGVGFYGACAIGRVSPDDALGRMWPYLAALAVALAIVVAVPWLSIGF
                     L"
     gene            3100..3816
                     /locus_tag="VKQ27_10500"
     CDS             3100..3816
                     /locus_tag="VKQ27_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HLY89404.1"
                     /translation="MDAAGLTAVRPLKRNLREQLVDMLGGDIINGRLQPGDLLPSEDV
                     LVTRYGVSRTVLREALNVLSGKGLLDARPKRGTVVRPKSAWSQLDPVVLGWRGGNVSR
                     SLDHLMDLRRMIEPAAAALAAQRRTAKDLARISAAYTAMERAKDAVQEFMDADLAFHV
                     ACLEATHNEFLSPIAHAIRTELMTSMRVTNRDPEENQKVSLPLHRTIRDAIAARDPDA
                     ARVAMQRHLDDTERRRRVGK"
     gene            complement(3820..4266)
                     /locus_tag="VKQ27_10505"
     CDS             complement(3820..4266)
                     /locus_tag="VKQ27_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY89405.1"
                     /translation="MASPSRPDRSITLEDRFGISDLFTRYTCALDAGEVDTIVDCFTE
                     DGALVSPAVGAHRGRAAIRAFAERFARFRANGSQLRHVISNLMMQVDGDRAHATCYLT
                     VFLTRDGNSSLMAPGCYDCELRRTDGVWRFQNRIVRHDHDYTLEGL"
     gene            4300..5589
                     /locus_tag="VKQ27_10510"
     CDS             4300..5589
                     /locus_tag="VKQ27_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89406.1"
                     /translation="MRNNRRFAIYIMLFLFLCIYSLDRVTMGVAGSTVAKELHIGPVA
                     LGYLFSSYLWLYAVLLLPVGAMTDRVGTQRMSALAAGFWSLSQALGGAATSLTFLLCT
                     RLGLGVFESAANPCAHRALREWTPRRERGLATAIWYSGTTAGPAVGSPMVGWLITEYG
                     WRSSFIVTGIIGFIWVVAWVPFYRSPDKARWLREPEREMILRERDVSEAVQGDSVGYR
                     GLLFDNATMWGLALTQGCINYTAYFFLAWLPSFLQASYGLTVLQASGYTAVPFALSTV
                     LALGLSYMCDRTLSASALHDGKRRYAVALGALMSGLIVLVPYAGSINLATVVLTISLT
                     FNTFAQSMNFALTNDRLRSTGDVGRAYAFFTLGGISFGIAGPIVTGYLVSLTGDFKVA
                     LVLCGGLSLAAAILVTSLTRQPMGERAVNGVAVAGAV"
     gene            5589..6410
                     /locus_tag="VKQ27_10515"
     CDS             5589..6410
                     /locus_tag="VKQ27_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY89407.1"
                     /translation="MAMPLPDISNPVRRRLRANELALGLIVRISRTGDIARIARASGH
                     DFVFIDTQHGMFSIETIGHIAQVALGCGVAPFVRARSVRDPDVPVLLDNGVTGIVFPD
                     VNTEEDAQVAVRACKFAPIGRRSVTTGYPYFDYRPVPSSDAIRLLNDNTLVVCMIESL
                     QGLANAEAIASVEGVDVVQVALTDLLADMGKPGAYGDPEAMEAVARVTRAALAHGKFA
                     GVGGDNEPARQRIFIRDGVRFISTQSDASLLLAGATRIADGLREIANNASLETKS"
     gene            6423..7409
                     /locus_tag="VKQ27_10520"
     CDS             6423..7409
                     /locus_tag="VKQ27_10520"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY89408.1"
                     /translation="MILSRRRFAGIVGGTALAMPAIRTRAADAIQLKFATADTMQDTS
                     YSVAQHFADEVAHRTNGKYAMQLFVNGALGTALNLANSLQTGILDCAILTTGWMQSFV
                     PSVQVVDLPFLFKDAATAAKILDGSVGQQIFADMEAKGFVGLEWGWYGWRQMETRDKA
                     VHTPDDLRGLKMRIQSGPVFVAMFKALGAIPVPLDGTEVYLALSQKTVDGLEFPLPTS
                     VNFKVYEVCKDVALTNHIYNAGILMVSKARWTQMTPADQTAFREAGQTIRGYWRDTIA
                     KASDSAADTLKQRGMRINAVDFKPFQEKMTSVYTDFKPKYPKLLDDILAQQS"
     gene            7406..8032
                     /locus_tag="VKQ27_10525"
     CDS             7406..8032
                     /locus_tag="VKQ27_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89409.1"
                     /translation="MSGQRGLLLATMEPPPAIEEEFQDWYDTEHFPERAGCPGFLTAR
                     RFICVDGWPRYLALYDLQDVQVLHGPDYTKFAGARYSPWTHRIMSRVWGQYRAEGVQL
                     YPGQALLGGNGAATRVMLCRFRQVPPDHAAPIEQTLRALDARVETAQARLYVATQPDG
                     VDYIGIVELRGNLPIGADAFGTAAKYLDLLNLYVPYTRRMAGAFPTET"
     gene            8029..9867
                     /locus_tag="VKQ27_10530"
     CDS             8029..9867
                     /locus_tag="VKQ27_10530"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLY89410.1"
                     /translation="MKQPVAILDAGVDVVVGTALVGELLAVITDVLGRTFWDAPLLWA
                     DEVSSLALSVIAFIGGAIAYRREQHVFIRTFVDLLPDRLRHASYAFADWLVLLLAAVC
                     CHASLGLLSARWDETTPILEMRGTWLALPLTVGMVVLGLYALIRLHAQPKRAVALGGA
                     VVFGLAVVIIAAHTWWLPALSTGAAVSCSIVVVLLTALLGVPIGFALILGTMLFLYSG
                     GLVGFIALPQNMVDGVSRFVLLALPFFILAGFVMESGGISRRLVMFVASLVGFLRGGL
                     MQVTVVSMYIVSGISGSKAADVTAVGLVMRDMLEEKGYDPAEAAAVLAASAAMGECVP
                     PSIAMLVLGSVTSLSTGALFAAGLLPAAVIAICLMLLIYVRSVRSDVQHSEHIGHGHR
                     LRLAADAIMPASMPAFLFAGIFTGFATPTELSAFAVAYGLLIAVLLYREIRLPGLGRI
                     IATSSTVAAMVLFTLAAAQSFSWVLSAAQVPQALATLLAAAHENAALFMICSIIGLVV
                     LGSLLEGLPALLILAPLLLPMAPALGINQLHYGIVLLIAMGIGAFLPPLGVGFYIACA
                     VARAPMGRSSHIMVPYVVVLLLGLVLVAFIPWLTLVLPRAFGFAGV"
     gene            complement(9943..10764)
                     /locus_tag="VKQ27_10535"
     CDS             complement(9943..10764)
                     /locus_tag="VKQ27_10535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY89411.1"
                     /translation="MKLGIHLPHAGEQATPTLIRRHAIRAEALGFADVWVSEHIIVPR
                     KEFPRSPLFYDPVVTLSWVAAVTQRVRLGTTVLVLPMRHPLPLAKELSTLHNLSEGRL
                     ILGAGVGWLEPEFAALGVPFRERGRRMDEGIAMMRAVWTQDPVTFEARYIPARIVDMT
                     MQPQPVSKIPLWFGGSSDAALKRTIRIGDGWHGSRETPDEAAPIIRRLRAERPGDDLT
                     ISMRIQWNGKDQAELRDRLAAYEAVGVEHVLVAPNNREVDDWDEVIEGAGRMVSA"
     gene            complement(10856..>11286)
                     /locus_tag="VKQ27_10540"
     CDS             complement(10856..>11286)
                     /locus_tag="VKQ27_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89412.1"
                     /translation="AIARLPTQKRAPRPSRPAGWNDPELFMPTMQDLEREARRSPVGR
                     ILLKICLDLAVVPAFCHSQFWNELFELITYFGSSVNQLMQEKARRRKAFANEQDRNPK
                     ATWDWLNLGRDAPRQILGFFIGEPPVNPFDPAAAKATAPP"
BASE COUNT         1785 a         3289 c         3936 g         2276 t
ORIGIN      
        1 gagcctcccg ccacgctgcc gcaaaatcag aagcggccgt atcggcccaa acaggaggat
       61 atcgtcgatg cagcgaggga aactgtcccg cagacgcgtc ttgacgggag cggcgggcgt
      121 ttccgccgca accattttgc actggcccgc caacgccgcc gagttcagct acaagcttgg
      181 ttcgtcatcg ccgatggagc atccggcgat ggctgcgcaa caggatgcag ccaacaagat
      241 caagcaggaa agcaacggac ggctggagat caccatctac ccgaacagcg tgctgggcgg
      301 tgatacagcg atgatcgcgc agacaatctc cggggcgatg gagatgtaca atctgccgat
      361 cgatctgctg gcgccgcgca atccggtgtg cggtctgcct ggcgtcggct ttgcatttcc
      421 tgattacgcc catgtctggg ctgcgatgga tggcgagttc ggcaatttcc tgcgcgccgc
      481 ggcggaggag attggccttt attgcctgga gaagggctac gatcacggct tccgccagat
      541 caccacgcgt accaagccga tcacgagccc ggatgacctg aaaggcttca agatccgcct
      601 gccggtcgcg ccgtatctga tctcgctgtt ccgtcacctg ggtgcgtcgc cgacggcgat
      661 caacttcaat gaggtctaca gcgcgctgca gactggcatt gtcgatggcc aggagaaccc
      721 gctggtgctg atcgacactg cgaagctgtt cgaggtgcag aagtactgca gcctgaccaa
      781 ccatgtgtgg gcgggtttcc acacctcgtt caatgttgcc gcgtggaaga agctgccacc
      841 tgatctacag gagatgtgcc accacaactt ctccgaggtc gcactcaagg aacgtgacgc
      901 cttcgtcacc atgaccaaga gcgagcagca gaacctgagc ggcaaagggc tcacgttcaa
      961 ttcgccggac accaagccgt tccgcgacgt gctggcgaag accggctttt atccggacat
     1021 gaagaaaacc gccggcgaca aggggtgggc gctgctggag aaatacgtcg ggccgctgac
     1081 gtcgtgagcc tcgacgcgac aactgttccg caggatgtgc ccgggtgggc cgtcctgcgc
     1141 cgctgcgcat tcctgatcga ccagggcatc ggcacggcct gcgaggccat tggcgccgca
     1201 ctggtactgg cggaggtctg catcctgttc gccggcgtgg tgtcgcgtta tgcgctcgac
     1261 agcccgctgt tctggaccga tgagctggcg aatttcctgt tcctgtggct gtccatgctg
     1321 ggcatggtgg tggcgctgcg gcgcgacggc catatgcggc tgaccacggt ggccaactgg
     1381 gttccgcccc atatcgcgcg ctggtttgcc gcagttgcct cgctggtagt gatcgccttt
     1441 gtgctggaag tgctgtggcc ggctacccag tacctggacc agcagcgcta tgtcgagctg
     1501 atcacgctgc agatctccga cggctatcgc gtcaccgcca tcgtcgtcgg catggcgctg
     1561 gccgcggtca tctcactgtt gcgcctgctg gagaccacaa cgtggcgcag cttctgcctt
     1621 gcggtgctga ctgtcggcgg catcgccgcg ctgttgtggg ccgggcgccc ggtgctgctg
     1681 tcgatgggct ttggcagcct gatcgtcttc ttcgtggtca tcgtcgccgc ctgcgtggcg
     1741 atcggtgtac cgatcggatt tggtttcggc attgcgacga tgtcgtatct tggcctgacc
     1801 agcgcgctgc cgctctcggt ggtggtgaac cgcatggacg agggcatgtc gaacctgctg
     1861 ctgctctcgg tgccgatgtt cgtgttcctt ggcctgctga tggagatggc aggcatcgcg
     1921 cgtgtgatgg tggcatttct cgccgcgctg gtggggcatg tgcgcggcgg gctgtcgtat
     1981 gtgctgctgg gcgcaatgta cctcgtgtcg ggcatatccg gctccaaggc ggccgatatg
     2041 gcagcggtgg cgccagtgct gttccccgag atgcagcgcc gcggttcgca tcccggggag
     2101 ctggcggcgc tgctggcgag ctccagcgca atgtcggaga ccatcccgcc gagcctggtg
     2161 ctgatcacca tcggttcggt cgccaacgtg tcgatcaccg cgctgttcac cggcggcctg
     2221 ctgccggcgg cggtggcggc ggtcgcactg ctggtgatga gctggtaccg ctcgcgccac
     2281 atggatatca gcggtggtga gcgcgcctcc accggccaga tcctgcggac tttcgtcatc
     2341 gcggtgcccg gcctgacgct gccgttcgtg atccgcgccg cggtgatcga tggggtggcg
     2401 accgcgaccg aagtctccac tgttggcgtg gtgtacacgg cggtgctggg gatgttcgta
     2461 tatcgacagt tcgactggcg gcggatctat ccgatcctgg tggatacgct gtcactcacc
     2521 ggcgcggtca tgctgatcat cggcaccgcc accgcgatgg cctgggcgct gactcagtcc
     2581 ggcttctccc aatggctggt tgcgactatg gccactgtgc cgggcggcaa gcttggcttc
     2641 cttgccatca cggccgtgac gttcgtgatc cttggcagcg tgctggaggg tatcccggcg
     2701 atcgtgctgt tcgggccgct gctgttcccg gtggcgaagg cgatgggcgt gcacgaggtg
     2761 cactacgcca tgatcatcat cctcgcgatg ggtatcggcc tgttcgcgcc accattcggt
     2821 gtcgggttct acggtgcgtg cgccatcggg cgcgtatcgc ccgatgatgc gctggggcgg
     2881 atgtggcctt acctggcggc gctggccgtc gcgctggcaa tcgtggtggc cgtaccgtgg
     2941 ctttcgattg ggttcctgtg agcacctgtc gtactctttg taatatgata tggtagccgc
     3001 tgaaccagca ggtaggctca tgagcataac cggggaaagc ggcgacatcg gcgtcgacgc
     3061 cgcgtgggcg tcggcgttca tcaaggtctg acgggcgcga tggatgctgc cggcctgacg
     3121 gctgtccgtc cgctgaagcg caatctgcgc gagcaactcg tggacatgct cggcggcgac
     3181 atcatcaatg gccgccttca gccaggtgat ctgttgccca gcgaggatgt gctggtgacg
     3241 cgctacgggg tcagccgcac cgtgctgcgc gaggcgctca acgtactgtc cggcaagggg
     3301 ttgctggatg cgcggccaaa gcgcgggacg gtggtgcggc cgaagtcggc atggagccaa
     3361 cttgatccgg tggtgctggg ttggcgcggc ggcaacgtca gccgcagcct ggatcatttg
     3421 atggacctgc ggcggatgat cgagcctgcc gccgcggcac tcgccgcgca acgccgtact
     3481 gcgaaggacc tggcgcgcat cagcgctgcc tataccgcga tggagcgggc gaaagatgcg
     3541 gtgcaggaat tcatggatgc ggatctggcg ttccatgtcg cctgcctgga ggcgacgcat
     3601 aacgaattcc tgtcaccgat cgcgcatgcg atccgcaccg agctgatgac gtccatgcgg
     3661 gtgacaaatc gcgatccgga ggagaaccag aaggtctcgt tgccgctgca ccggaccatt
     3721 cgcgacgcga tcgccgcacg ggatccggat gcggcacggg tggcgatgca gaggcacctg
     3781 gatgataccg agagacggcg gcgggtaggg aagtagccgc tacaacccct caagcgtgta
     3841 gtcgtgatcg tggcgcacga tgcggttctg aaagcgccag acgccgtcgg tgcggcgcag
     3901 ttcgcagtcg taacagccgg gcgccatcag gctgctattg ccatcgcgcg tgaggaacac
     3961 cgtgagatag caggtggcgt gggcgcgatc gccgtcgacc tgcatcatca ggtttgagat
     4021 cacgtgacgt aactgtgagc cgttggcgcg gaaacgggcg aagcgttcgg cgaaggcgcg
     4081 gatggcagca cggccccggt gcgcgccgac ggcgggactg accagcgcgc catcctcggt
     4141 gaagcagtcc acgatcgtgt ccacctcacc ggcatccagc gcgcaggtgt agcgggtgaa
     4201 caagtcactg atgccgaaac gatcctccag cgtgatcgag cggtctggac gcgatggtga
     4261 tgccatgttc atatcataag actaattgga ggagacagta tgcgcaacaa ccggcgcttc
     4321 gccatctaca tcatgctgtt cctgttcctg tgcatctact cgctggatcg cgtgaccatg
     4381 ggcgtcgccg gcagcacggt tgccaaggag ctgcatatcg ggccggtggc gctgggatac
     4441 ctgttctcct cctatctttg gctctacgcc gtgctgctgt tgccggtcgg tgcgatgacg
     4501 gatcgggtcg gtacgcagcg aatgagcgcg ttggctgccg ggttctggtc cctgtcgcag
     4561 gcgctgggcg gcgctgccac gtcgctgacg tttttgttgt gcacgcggct tggcctgggc
     4621 gtgttcgaga gcgccgccaa tccgtgcgcg catcgtgcgc tacgtgagtg gacaccgcgg
     4681 cgtgagcgcg ggctggcgac ggcgatctgg tacagcggga cgactgcggg ccctgcagtc
     4741 gggtcgccga tggtgggctg gctgatcacc gagtatggct ggcgctcatc gttcatcgtg
     4801 acaggcatca tcgggtttat ctgggtggtg gcgtgggtgc cgttctaccg ctcacccgac
     4861 aaggcgcgct ggctgcgcga acccgagcgc gagatgatcc tgcgcgaacg cgacgtatcc
     4921 gaggcggtgc aaggcgattc cgtcgggtat cgagggctgc tgttcgacaa cgccacgatg
     4981 tggggcctgg ccctgacaca gggctgcatc aactacaccg cgtatttctt ccttgcctgg
     5041 ctgcccagct tcctgcaggc ctcgtatgga ctgaccgtgc tgcaggcgag cggctacacc
     5101 gcagtgccgt ttgctttgag cacggtgctc gcactggggt tgagctatat gtgcgatcgg
     5161 acgctgtcag cctcggcgct gcatgatggc aaacgacgct atgcggtggc gctgggggca
     5221 ctgatgtcgg ggctgatcgt gctggtgccg tatgccggct cgatcaatct ggcgacggtg
     5281 gttctgacaa tctcgctgac attcaacacg ttcgcgcaat cgatgaattt cgcactgacc
     5341 aatgatcggc tgcggtcgac cggtgatgtc ggacgcgcct atgcgttctt cacgctcggt
     5401 ggcatttcgt tcggcatagc tgggccgatc gtgaccggct acctggtgtc gctcaccggc
     5461 gacttcaagg ttgcgctcgt gttgtgtggc ggactttcgc tcgcggcggc gatcctggtc
     5521 accagcctga cgcgacagcc gatgggcgaa cgtgccgtca acggcgttgc ggtggcaggc
     5581 gcagtgtgat ggcgatgccg ctgcccgata tcagcaatcc cgttcgccgg cggttgcggg
     5641 cgaatgagct ggcactgggg ctgatcgtgc gaatctcgcg caccggcgac atcgcgcgga
     5701 tcgcgcgtgc gtcggggcat gatttcgtgt tcatcgatac gcagcatggg atgttctcga
     5761 tcgagacgat agggcatatc gctcaggtgg cgttgggctg cggcgtggcg ccttttgtgc
     5821 gggcacgcag cgtgcgggat ccagacgttc cggtcctgct ggataacggc gttactggaa
     5881 tcgtgtttcc ggacgtgaat acagaagagg atgcgcaggt cgcagtgcgt gcctgcaagt
     5941 tcgcgccgat cgggcggcgg tctgtcacca cgggataccc gtatttcgat tatcgaccgg
     6001 tgccgtcatc ggatgcgatc cggctgctga acgacaatac gttggtggtc tgcatgatcg
     6061 agtcgttgca ggggcttgcc aatgccgagg cgatagcctc ggtcgagggc gtggatgtgg
     6121 tgcaggtggc actgaccgat ctgctggcag acatgggcaa gcccggagcc tatggcgatc
     6181 ccgaggcgat ggaggcggtg gcacgtgtta cgcgcgccgc tctggcgcac ggcaaatttg
     6241 ccggcgtcgg cggcgacaac gagccggcac ggcagcgcat cttcatccgt gatggcgtgc
     6301 ggttcatctc cacgcagtcg gacgcatcgc tgctgctagc gggagcgacg cgcatcgccg
     6361 atgggttgcg cgagatcgcg aacaatgcct cgctggagac caaatcataa ggaggaaacg
     6421 ccatgatttt atcacgccgt cgattcgccg gcatcgtggg cggtaccgcg ctggcgatgc
     6481 cggcgatacg cacacgggcg gccgacgcca tccaactgaa gttcgccact gccgatacga
     6541 tgcaggatac gtcgtacagc gtggcgcagc acttcgcgga cgaggttgcc catcgcacca
     6601 atggcaagta tgcgatgcag ctttttgtca acggtgcgct gggaacggcg ctgaacctgg
     6661 cgaacagcct gcagaccggc attctggatt gcgccatcct gacgacggga tggatgcaat
     6721 cgttcgtgcc gtcggtgcag gtcgttgacc tgccattcct gttcaaggat gcggcgaccg
     6781 cggcgaagat ccttgatggc tcggtgggac agcagatctt tgcggatatg gaggcgaagg
     6841 gctttgtcgg ccttgagtgg ggctggtacg gctggcggca aatggagaca cgcgacaaag
     6901 ccgtgcacac accggacgat ctacgcggat tgaagatgcg cattcagtcg gggccggtat
     6961 tcgtggcgat gttcaaggcg ctgggcgcga tcccggtgcc gctggatggc accgaggtgt
     7021 atctggcgct gtcgcagaag acggtggacg ggttggaatt cccgttgccg acgtcggtga
     7081 acttcaaggt gtacgaggtc tgcaaggatg tggcgctgac caatcacatc tacaacgcgg
     7141 ggatattgat ggtgagcaaa gcgcgctgga cgcagatgac gccagccgat cagaccgcgt
     7201 tccgcgaggc gggacagacc atacggggct attggcgtga cacgatagcc aaggcgtccg
     7261 acagcgctgc cgatacgctc aagcagcgcg gcatgcggat caacgccgtc gatttcaagc
     7321 cgttccagga gaagatgacg tctgtgtata cggacttcaa accgaagtat ccgaagctgc
     7381 tcgacgatat cctggcgcag cagtcatgag cgggcagcgc ggcctgctgc tggcgacgat
     7441 ggaaccgccg ccggcgatcg aagaagagtt ccaggactgg tatgacaccg agcattttcc
     7501 ggaacgtgcc ggctgccccg gcttcctgac ggcgcggcgc ttcatctgtg tcgatggctg
     7561 gccacgctac ctggcgctgt atgacctgca ggacgtgcag gtgctgcatg ggccggacta
     7621 cacgaagttt gccggcgcgc gatattcgcc ctggacgcat cgaatcatgt cgcgtgtctg
     7681 ggggcagtat cgcgccgagg gcgtgcagct ttatcccggc caggcattgc ttggcggcaa
     7741 cggcgcagcg acgcgagtga tgctgtgccg attccggcag gtgccaccag atcacgcagc
     7801 accgatcgaa cagacgctgc gtgcgctgga cgcgcgtgtg gaaaccgctc aggcacggct
     7861 ttacgtggct acccagccgg acggtgtgga ctatatcggc attgtggaat tgcgtggcaa
     7921 tctgcccatt ggggcggacg cgttcggcac ggcggcgaag tatctcgatc tgctcaacct
     7981 ttatgtcccc tacacgcgcc gcatggctgg cgcgtttccg acggagacat gaagcaaccc
     8041 gtcgcgatcc tcgatgcggg ggtcgatgtc gttgttggca cagcgttggt gggcgagttg
     8101 ctggcggtca tcaccgacgt gcttggccgc accttctggg atgcaccgct gctgtgggct
     8161 gacgaggttt ccagcctggc actgtcggtc atcgcgttca tcggcggcgc cattgcctat
     8221 cggcgcgagc agcacgtgtt catccgcact tttgtcgatc tgctgcctga tcggctgcga
     8281 catgcaagct atgcctttgc cgattggctg gtgctgctgc tcgcggcggt ctgttgccat
     8341 gcttcgctcg gcttgctctc ggcgcgctgg gacgagacga caccgatcct tgagatgcgc
     8401 ggcacgtggc tcgcattgcc gcttaccgtt ggcatggttg tgcttggcct ctatgcactg
     8461 atccgattgc acgctcagcc gaagcgcgcc gtggctctcg gtggtgccgt ggtgtttggc
     8521 ctggccgtcg tgatcattgc cgcccatacg tggtggttgc cggcgctatc gacgggcgct
     8581 gccgtgtcat gctcgatcgt tgtggtgttg ctgacagcgc tgcttggcgt gccaatcggg
     8641 tttgcgctga tcctcggcac aatgctgttc ctgtacagcg gcggattggt ggggttcatc
     8701 gcgctgcctc agaacatggt cgatggggtc agccggtttg tcctgctggc gcttccgttc
     8761 ttcatattgg cggggtttgt gatggagtcg ggcggcatct cgcgccggct cgtcatgttc
     8821 gtcgcatcgc tggtcgggtt cctgcgcggc ggactgatgc aggtcaccgt ggtcagcatg
     8881 tacatcgtgt ccggcatatc ggggtccaag gcagccgacg tcacagccgt cggcttggtg
     8941 atgcgcgaca tgctggagga gaaaggctac gaccctgcgg aggccgcagc cgtgctggca
     9001 gcctcggcgg cgatgggcga atgcgtgcca ccgagcatcg cgatgctggt gctcggctcg
     9061 gtgacttcgc tgtccaccgg cgcactgttc gccgctggat tgctaccggc ggcggtcatc
     9121 gcgatctgcc ttatgctgct gatctatgtg cgatcggtgc ggagcgatgt gcagcattcc
     9181 gagcatatcg gccatggtca tcgcctgagg ctggcggcag atgcgatcat gccggcatcc
     9241 atgccggcgt tcctgttcgc gggcatattc accgggtttg cgaccccgac ggagttatcc
     9301 gccttcgcgg tggcgtatgg gctattgatc gcggtgttgc tgtatcgcga gatccggctg
     9361 cccggcctgg ggcggatcat cgccacatca tcgactgtcg cggcaatggt gctgttcacg
     9421 ctggcggcgg cgcagtcgtt ctcctgggtg ctgtcggcgg cgcaggtgcc tcaggcattg
     9481 gcgacgctgc tcgcggcggc acacgagaac gcggcgctgt tcatgatctg ctcgatcatc
     9541 gggctggtcg tgctcgggtc gctgctggag gggctgccgg cactgctgat cctggcgccg
     9601 ttgctgctgc caatggctcc agcgctgggc atcaaccagc tgcactatgg catcgtgctg
     9661 ctgatcgcta tgggcatcgg tgctttcctg ccgccgctgg gggtgggttt ttacattgcg
     9721 tgcgcagtgg cgcgcgcgcc gatgggacga tcatcgcaca tcatggtgcc ttacgtggtg
     9781 gtcctgctgc tgggccttgt gctggtggcg ttcattccct ggttgacgct cgtgttgccg
     9841 cgcgcgtttg ggtttgccgg ggtgtagtct gcggcgaccg cgatcgaaag acgtgggtac
     9901 tcgcgccaag cgcgagcatg acgaagggac gatcgccgct gtctaggcgc tcaccatgcg
     9961 accggcgcct tcgatcactt cgtcccagtc atctacctcg cggttgttcg gcgcgacaag
    10021 gacgtgctcg acgccgacgg cttcgtaagc cgcaaggcgg tcgcgcagtt cggcctggtc
    10081 cttgccgttc cactggatgc gcatcgagat agtgaggtca tcgccgggac gttcggcgcg
    10141 gaggcgcctg atgatcgggg cggcttcgtc gggggtctcg cggctgccgt gccagccgtc
    10201 gccaatgcgg atggtgcgct tcagcgccgc gtcggagctg ccgccgaacc agagcgggat
    10261 cttgctgacc ggctgcggct gcatcgtcat gtcaacgatc ctggcgggga tgtatctggc
    10321 ctcgaaggtg accgggtcct gggtccagac ggcgcgcatc atggctatgc cttcgtccat
    10381 acggcggccg cgttccctga agggaacgcc gagggcggcg aattccggct ccagccagcc
    10441 gacaccggcg ccgaggatca ggcggccttc cgacaggttg tgcagggtgg agagctcctt
    10501 ggcgagcggc aggggatggc gcatcggcag caccagcacc gtggtgccga gacggacacg
    10561 ctgcgtgacg gcggcgaccc agctcagggt gacgacgggg tcgtagaaca gcggcgagcg
    10621 ggggaattcc ttgcgcggga cgatgatgtg ctcgctgacc cagacgtcgg caaagccgag
    10681 cgcctcggcg cggatggcgt ggcggcggat cagggtgggg gtggcctgct cgccggcgtg
    10741 gggcaggtgg atgccgagct tcatgcgcgt tcccttcggt tgttgcgcga agaatgcgcc
    10801 gaaccgagcg agcggccaat cgcggtgaag caggcatgag gcagtgcccg atggttcagg
    10861 gcggggcggt ggctttggcg gcggcagggt cgaacgggtt gaccggtggc tcgccgatga
    10921 agaagccgag gatctggcga ggggcgtccc ggcccaggtt gagccagtcc caggtggctt
    10981 tggggttgcg gtcctgctcg ttggcaaatg ccttacggcg gcgggctttt tcctgcatca
    11041 gctggttcac gctgctgccg aaatacgtaa tgagctcgaa cagctcgttc cagaactggc
    11101 tgtggcaaaa ggcggggacg acggcgagat ccaggcagat cttgagcagg atgcggccga
    11161 cggggctgcg gcgcgcctcg cgctcgaggt cctgcatggt gggcatgaaa agctcggggt
    11221 cattccagcc ggcggggcgg gaggggcgtg gggcacgctt ttgcgtgggc aggcgcgcga
    11281 tggctg
//
LOCUS       DATGEM010000329         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_421999, whole genome shotgun sequence.
ACCESSION   DATGEM010000329 DATGEM010000000
VERSION     DATGEM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421999"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VKQ27_10545"
     CDS             <1..274
                     /locus_tag="VKQ27_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89413.1"
                     /translation="VDRARTQPEISQARGVWVARMAIAVTLLGVVMSHAVLLRYKINF
                     SGTRPEVRYWLSQKIQPLLCPRARLTPDVKLDNFIPLVTPNCPPLW"
     gene            366..1091
                     /locus_tag="VKQ27_10550"
     CDS             366..1091
                     /locus_tag="VKQ27_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY89414.1"
                     /translation="MSDSNRIAVTAALLGDPARANMLAALMDGRALTAKELAYAAHVT
                     PQTASGHLAKLSDGGLLAGEKQGRHRYYRLASPLVGQMLEGVMAVSGPEVPRATTWRG
                     GEALRTARTCYDHLAGRLGVALSDALTDRGHVALGADGGEVTEAGLRFLSEFGAAPGA
                     GRRVFCRPCLDWSERRPHLAGRVGAALACRCFELGWIKRQRDTRAVAITSAGREGLSC
                     TFGITLDDEARTPAGGERPVFAD"
     gene            complement(1088..1864)
                     /locus_tag="VKQ27_10555"
     CDS             complement(1088..1864)
                     /locus_tag="VKQ27_10555"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HLY89415.1"
                     /translation="MSYRPPRRPQFFYTTAPLPCPYLAGRTERKVVTEITGPDADSLH
                     DRLSRAGFRRSHNIAYAPVCPSCQACIPIRIPVATFQQDRTQRKIWRANLMLEGFEVP
                     ARATAEQFQLFQRYQSARHGDGDMATMSFYDYRAMVEDTPIETFIIEFRDPDDRMVSA
                     CLADKLGDGLSAVYSFFAPALERRSLGTHAILWLIERARSLGLPYVYLGYWVPESRKM
                     AYKSRFRPAEVLAGGSWRMLTDADTGEVVPHEALVSESAS"
     gene            complement(1861..>2213)
                     /locus_tag="VKQ27_10560"
     CDS             complement(1861..>2213)
                     /locus_tag="VKQ27_10560"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLY89416.1"
                     /translation="LFGLMTLGLGFPMMGILPFIPFCYHFLFVAGSGATPGQQAMGLA
                     VRRNHDLGPPTALQALVYTLVFYVTLALTGLLLLIALFTIRKRTLHDIASGLVVVRTR
                     ALTGSAGSWNMQGG"
BASE COUNT          395 a          664 c          786 g          368 t
ORIGIN      
        1 tgtcgatcgc gcccgcacgc agcccgagat cagccaggcg cgcggggtgt gggtggcgcg
       61 gatggcgatc gccgtaacgc tgctcggcgt cgtcatgtcc catgcggtcc tgctacgata
      121 caaaatcaac tttagtggaa cgaggccgga ggtgcgttac tggctgtcac aaaagatcca
      181 gccgttgctt tgtccccggg cgcgactgac gccggacgtg aaacttgaca acttcatccc
      241 gctggtgacg ccaaactgcc cgccgctatg gtgatggccg cgacaattcg gcgtgggccg
      301 aactatcggt gcctgcgctg gcggtcgtgc tgaattatgt tcatagcggc aatccggagg
      361 gcgtcatgag cgactcaaat cgcatcgccg taacggccgc gctcttgggc gatccggccc
      421 gggcaaacat gctggctgcg ttgatggacg gccgcgcgct gaccgcgaag gaactggcgt
      481 atgccgcgca cgtcacgccg cagaccgcga gcgggcatct ggcgaagctg tccgacggcg
      541 ggctgctggc gggcgagaag caggggcggc atcgctatta tcgtctggcc tcgccgctgg
      601 tcggacagat gctggaaggc gtgatggcag tgtccggccc cgaggtgccg cgtgcaacga
      661 cctggcgtgg cggggaggcg ctgcggaccg cgcgcacctg ctacgatcat ttggccgggc
      721 ggctaggtgt agccctgtcg gatgctctga ccgaccgtgg acatgtggca ctgggcgcgg
      781 atggcggcga ggtgacggaa gcaggcttgc gtttcctcag cgagttcggt gccgcaccgg
      841 gagccggacg gcgtgtattc tgccggccgt gtctggattg gagcgagcgg cggccacatc
      901 tggccgggcg tgtaggagcg gcgctcgcat gccggtgctt cgagttgggc tggatcaaac
      961 ggcagcggga tacgcgggcg gtggcgatta ccagcgccgg gcgggagggg ctcagctgca
     1021 cattcggcat aacgttggat gacgaagcac gaactccagc tgggggtgag cgtcccgtct
     1081 tcgctgatta actcgcgctc tctgacacca aagcctcgtg cggcacgacc tcgccggtgt
     1141 ctgcatcggt gagcatccgc cacgagccac ctgccagcac ctcggccggg cggaaccgcg
     1201 acttgtaggc catcttgcgg ctctccggca cccagtaacc gagatacacg tagggcagtc
     1261 caagtgatcg cgcgcgctcg atcagccaaa ggatcgcgtg cgtgcccagc gagcgccgct
     1321 ccagcgccgg cgcgaagaat gaatacacag ccgacagccc gtcgccgagc ttgtccgcga
     1381 ggcaggcgct caccatccga tcgtccggat cgcggaactc gatgatgaag gtctcgatcg
     1441 gcgtgtcctc caccatggcg cggtagtcgt agaaactcat ggtggccatg tcgccatcgc
     1501 catgtcgcgc cgattggtag cgctggaaca gctggaactg ttcggcggtg gcacgggcag
     1561 gaacctcgaa gccctcgagc atcaggttgg cgcgccagat cttccgctgc gtgcggtcct
     1621 gctggaacgt ggcgaccgga atgcggatgg ggatgcaggc ctggcaggac gggcagaccg
     1681 gcgcgtaggc gatgttatgg ctgcggcgga agccggcgcg cgacaggcgg tcgtgcagcg
     1741 agtcggcatc ggggccggtg atttccgtca cgaccttgcg ctcggtccgt ccggccagat
     1801 aggggcaggg cagaggggcc gtcgtgtaaa agaactgcgg ccggcggggt ggtcggtagc
     1861 tcatccgccc tgcatgttcc aggagccggc gctgccggtc aacgccctcg tacgcactac
     1921 gacaagtccg ctggcaatat cgtgcaaggt gcgcttgcgg atggtgaaca gcgcgatcag
     1981 cagcagcagg ccagtgagcg ctagagtgac atagaacacc aacgtgtaga ccagcgcctg
     2041 caatgccgtt ggtgggccga gatcgtggtt acggcggacg gccagaccca tcgcctgctg
     2101 gcctggcgtc gcgcccgaac cggcgacgaa caggaaatga taacagaatg ggatgaacgg
     2161 caggatgccc atcatcggaa agccgaggcc gagcgtcatc agtccgaata gca
//
LOCUS       DATGEM010000330         9589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_577299, whole genome shotgun sequence.
ACCESSION   DATGEM010000330 DATGEM010000000
VERSION     DATGEM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577299"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VKQ27_10565"
     CDS             <1..441
                     /locus_tag="VKQ27_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLY89417.1"
                     /translation="VVPGWVSDSSFLAGYGAAQAVPGPLFTFSAYLGAVANIPPGGVA
                     GAMIALVAIFVPGILALMGTLPFWHGLRARSDARAAMAGINAAVVGLLAAALYNPVWI
                     SAVQSSADFAVAAAGFVLLVVWRAPPLLVVLACAAAGVGLAVVA"
     gene            complement(575..1066)
                     /locus_tag="VKQ27_10570"
     CDS             complement(575..1066)
                     /locus_tag="VKQ27_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89418.1"
                     /translation="MHLRLAVLCTFLVCGRAWAACTGQPYDQLDFWLGAWHDPAAPAA
                     EHYTARRTAGGCAVEEVLTGANGQVQGLGLSGWDTERNQWRQLWVDKDRIVTTYLGGP
                     AADGTFVLTSEPKASGTRWQYTYRNIRPNSIDADYASRVGDAGPWKTVWSGHFERIGP
                     AEK"
     gene            complement(1129..4257)
                     /locus_tag="VKQ27_10575"
     CDS             complement(1129..4257)
                     /locus_tag="VKQ27_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY89419.1"
                     /translation="MSISTPFIKRPIATSLVMAALVLVGIAAYPLLPVAPLPRVDFPT
                     IVVTGQLPGASPETMAATVAQPLETQIAQIPGVSQLTSSSILGVTSVTVQFDLDRSID
                     GAAGDIQAAIQDAAGQLPKTMPGPPTYRKVNPSDAPIMILAAQSEAMPLIDLDDYAEN
                     VLSQQISQISGVAQVLIGGQQKRAVRIEVDPEKIAAKGMTLEDVRTLLQSATVNAPKG
                     SIDGEHQAFTVYANDQLTRADQYKDVVLAYRNGAPIRVSDIGHAIDGPENRMLAAWQN
                     GKRGILLIIFKQPGANVIDTVERIKTALPRLEASIPPAIHITTIMDRTVTIRASVKDV
                     QFTLMLAVCLVVMVIFLFLRNLWATIIPTTTVPVALICTFAFMYLLNYSLDNLSLMAL
                     TIAVGFVIDDAIVMLENIFRHVEEGMKPMEAAIKGAGEIGFTIVSISFSLVAVFIPLL
                     LMGGIVGRLFREFAVTVTVAVMVSAFVCLTLTPMMCSRFLHHDSGKHGLLYRAIEAVF
                     TGMNSFYRRTLDIALKFHFITLLVFLGTVALSAYLFIIIPKGLFPAQDNGILIGITEA
                     RQDISFKEMMNVQQRLADIIAQDPDTAAYVSSMGGGFAGATNNQGRLFISLKPIDQRV
                     GGTVQDYINRLRPKIAKVSGGKLFLQPIPDVRVGARLAKTEYQYTLQDANLEELYDWA
                     PKILGSLQKLSMLRDVTSDQQNGGTTATLVIDRDAAGRFGIQPQVIDDTLYDAFGQRQ
                     ITQYFSQVNSYHLILEVPPEQAGDPATLDKLYVRSSNGQAVPLSTFVHWSTAPVQPLS
                     ISHQSQFPAVTISFNLAAGVALGDAVNAIQDAVRQMGAPITVAGSFQGTAQAFQSSLA
                     TLPYLLAAAFISVYIILGILYESYILPLTILSTVFSAGVGALLTLLLVHYDLTLIATI
                     GLILLIGIVKKNGIMMVDFAITAERRDGMSPQNAIRQACLLRFRPILMTTMAAMLTGV
                     PLMLGHGAGSEFRRPLGFAMVGGLALSQLLTLYTTPVIYLYLDRFQRLISPARRRLPH
                     LAEKMGEAATAD"
     gene            complement(4265..5374)
                     /locus_tag="VKQ27_10580"
     CDS             complement(4265..5374)
                     /locus_tag="VKQ27_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY89420.1"
                     /translation="MLVFLGWAACVPALAQQARPSATAVPVSVTKAKRQDVPVWLRGI
                     GTVQALNTVVVRARVDGTLMQVPVTEGQLVKKGALLAVIDPRPYQASLDQAQAKKGAD
                     QAQLGNAKLDLQRYASLAQSSFASRQQVDTQTMQVAQLTASLKGDDAAIETAQLNLSF
                     CYITAPFDGRIGLRQLDPGNIVRATDLAGIMMITQVRPIAVVFTLPQRQLPEIVEAMG
                     KGTLPVTAWSNDEHEQLSEGSLLTPDNMIDPATGTVKLKATFANENDRLWPGQFVEAR
                     VLVRTEPQVVTVPSQAVLHGMANLYVYMVKPDSTVLRQSVEAVDRGDVMVITKGLQAG
                     QEIVLDGQSRLDNGMLITAMTPSAPASASPAQTGG"
     gene            complement(5593..6174)
                     /locus_tag="VKQ27_10585"
     CDS             complement(5593..6174)
                     /locus_tag="VKQ27_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY89421.1"
                     /translation="MRNDGTLDRRKVASVLLRDPGDLLVVSGLGAPSYDAAAAGDRPL
                     NFYLWGAMGGAAMIGLGIALAQPSRRVLVLTGDGEMLMGMGSLATIAAARAPNLAIAV
                     LDNARYGETGSQPSHTGLTTDLAAVAAACGWSNTTTARSMDEVEALRIRLRQEHLFAV
                     LRIAPDEPPRHLPPRDGGYLADRFRRALGVSAD"
     gene            complement(6257..6775)
                     /locus_tag="VKQ27_10590"
     CDS             complement(6257..6775)
                     /locus_tag="VKQ27_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HLY89422.1"
                     /translation="MPAQSWQGEVFAALKAARITQVGYVPDAGHAHLIASAHADPEMH
                     TVVLTTEEEGIALAAGAWIGGQRAVLLMQSSGVGNCINMLSLPVNCRMPFLTLVTMRG
                     EWAEFNPWQVPMGTATPAVLTAAGVHVRRADQPGDVAEIVAASAAFAFDSAVPVAVLF
                     SQRLIGAKVFVK"
     gene            complement(6864..7577)
                     /locus_tag="VKQ27_10595"
     CDS             complement(6864..7577)
                     /locus_tag="VKQ27_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY89423.1"
                     /translation="MIELYGMGSPNVVKIYIALEELGLSYNVHPVDVFTGKQFDAQFL
                     KLNPMAKVPVITDSDGPGGKPITLFESGAILQYLAEKTGKLLPKDMAAKYEAIEWMMV
                     QMTTLGPMFGQHVHFVRFAPAGNDYSKSRYTTQVHRVLEVMDQRLASTHWLGGAEYGI
                     ADIATYPWARNIPALLGNDAAAKYPNLMRWVGVINERPAVKKALAAVEDVRARTTQFD
                     KAQANDLDKMFGRGQFAAA"
     gene            7691..>9589
                     /locus_tag="VKQ27_10600"
     CDS             7691..>9589
                     /locus_tag="VKQ27_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY89424.1"
                     /translation="MKFERLAAGWEPRFTPSTTVERSAVQSEAFVFGSFTLMPAERTL
                     LDGDMPLRLGSRALDILVALVENAGETVRKDELMARAWPDTVVDEASLRVHIAALRKT
                     LGDGHAGRRFIANIPGRGYVFVAVATRQHNAVIEPPRAAITGSNIPASLTRIIGRDNV
                     IAMLSEQIARRRLLTIVGPGGIGKTTIAAAVAEAARPSFPDGLWYVGLASVPSAELVP
                     TAVAAVLGISLPATDPIAGLVAWLRDRQALIILDNCEHVVEAAAALAESIVRSTPNVR
                     LLATSREPLRAVGEWRHRLTPLEFPPHANGLAAEDALQYPAIQLFNERAVATADEFLL
                     KDADVPALVEICRRLDGVPLALELAAAHVGVLGIKGLAERLDDRLALLIQGRRTALPR
                     HQTLRATLDWSYDLLPADEQVILRRLAAFQGEFTMEAASAVATDAAISSDDVVNAIAS
                     LVDKSLVSADIGGDVTYYHLLEMTRSYAQERLAGGGERDRIMRRHAEYFREVFAASHA
                     GADVRPKEEWLRHYGRHIGNLRAALDWAFGRGGDVALGVVLAAAATDFWCALALLSEC
                     CDWGTKAIAQLGAAEGTRDEMRLQCSLGQSYTYSKGMRLDAKSALNRAAVLAEELGEL
                     QYQVRIYYA"
BASE COUNT         1621 a         3120 c         3052 g         1796 t
ORIGIN      
        1 gttgtgccgg gctgggtgtc ggacagctcc ttcctcgccg gctatggcgc ggctcaggcg
       61 gtgccggggc cgttgttcac gttctcagcg taccttggtg ctgtcgccaa tatacccccg
      121 ggtggggtgg cgggcgcgat gattgccctg gtggcgatct tcgtgcccgg gatcctggcg
      181 ctcatgggca cgctgccatt ctggcacggg ttgcgtgcgc ggtccgatgc aagggcggca
      241 atggctggca tcaacgccgc cgtggtcggc ttgttagccg cggcgcttta caatccggtc
      301 tggatcagcg ccgtgcagag ttccgctgac ttcgcggtgg cggctgcggg ctttgtgctg
      361 ctggtggtct ggcgcgcacc tccgctgttg gtcgtgctgg cctgtgcggc ggccggcgtc
      421 gggttggcgg ttgtcgcctg ataccagcgc ccaatggaga cgactcattg ctcgacggtc
      481 aaacgacgtg gatgccgggc cttcgccggg catgacgaga accgctgatc ggtccctctc
      541 aatgaggtct gcgatgaggc gcggcggcgg ccggttattt ttctgccgga ccgatccgtt
      601 cgaagtgccc cgaccatacc gtcttccagg gtccggcgtc gccgacacgc gaggcataat
      661 cggcgtcgat gctgttgggc cggatgttcc ggtaggtgta ctgccacctg gttccgctgg
      721 ctttcggctc ggacgtcagg acgaacgtgc cgtccgcagc cggaccgccc aggtaggtcg
      781 tcacgattct gtccttgtcg acccagagct gtcgccactg gttccgttca gtgtcccagc
      841 cggacaggcc gagtccctgg acctgaccgt ttgcgccagt cagtacttcc tcgacggcgc
      901 agccaccggc ggtgcgcctg gctgtgtaat gttcggccgc tggtgcggcc gggtcgtgcc
      961 atgcgcctaa ccagaaatcg agctggtcat agggctggcc ggtgcacgcg gcccaagcgc
     1021 gtccacagac caagaaggtg cacagcacgg ccaaccgcag atgcatcaca gcagcctcat
     1081 cgcatgcccg tgccgtgcca gaacgctgct actcttgcgc gaccccggtc agtcggcggt
     1141 tgccgcctcg cccatcttct ccgcaaggtg tggcaggcgc cgccgcgccg gcgagatcag
     1201 ccgctggaag cggtccaggt acagatagat caccggcgtc gtgtacagcg tcagcaactg
     1261 gctcagcgcc aacccgccca ccattgcgaa gcccagcggc cggcggaact ctgatccagc
     1321 cccgtgcccc agcatcagcg gcacgcccgt cagcatggcc gccatcgttg tcatcagaat
     1381 cggccggaag cgcagcagac acgcctgccg tatggcattc tgtggcgaca tcccgtcacg
     1441 ccgttcggca gtgatcgcga agtccaccat catgatgccg ttcttcttca cgatgccgat
     1501 gagcaggatc agcccgatcg tcgcgatcag cgtcaggtcg tagtggacca ggaggagcgt
     1561 cagcagagcg ccgacgcccg ccgagaacac ggtggacagg atggtcagcg gcaggatgta
     1621 gctctcatac aggatgccga ggatgatgta gacgctgatg aatgccgcgg cgagcaggta
     1681 gggcagcgtc gccagcgagc tctggaacgc ctgcgcggtg ccctggaatg agccggcaac
     1741 ggttatcggc gcgcccatct gccgcacggc atcctggatc gcgttcaccg cgtcgcccaa
     1801 tgccactcct gccgccaggt tgaagcttat ggtcactgcc gggaactggc tttggtggct
     1861 gatggacaga ggctgcaccg gtgcggtgct ccagtgcacg aaggtcgaaa gcggcaccgc
     1921 ctgaccgttg gatgatcgta catacagctt gtcgagcgtc gccggatcgc cggcctgttc
     1981 cgggggcact tcaaggatca ggtgatagga attcacctgc gagaagtatt gcgtgatctg
     2041 tcgctgcccg aacgcgtcgt acagcgtgtc gtcgatgacc tgcggctgga tgccgaaccg
     2101 acccgccgca tcgcggtcga tcaccagcgt cgcggtggtg ccgccgttct gctggtcgct
     2161 cgtcacgtcg cgcagcatcg acagcttttg cagcgagccg aggattttcg gtgcccaatc
     2221 gtacaactcc tccaggttcg cgtcctggag cgtgtactga tactcggtct ttgccagccg
     2281 cgcgcccacg cgcacgtcgg ggatcggctg aaggaacagt ttgccgcctg acaccttggc
     2341 gatcttcggc cgcagacggt tgatgtaatc ttgcactgtg ccgccgaccc gctgatcgat
     2401 cggcttcagg gaaatgaaca gccgcccctg gttgtttgtc gcgcccgcga atccgccacc
     2461 catcgaggac acgtacgccg cggtgtccgg gtcctgcgca atgatgtccg ccagccgctg
     2521 ctgcacattc atcatctcct tgaaggagat gtcctgacgt gcctcggtga taccgatcag
     2581 gatgccgttg tcctgcgccg ggaacagacc tttcggaatg atgatgaaca ggtaggcact
     2641 cagcgccacg gttccgagga agacgagcag cgtgatgaag tggaacttca acgcaatgtc
     2701 cagcgtgcgc cggtagaatg agttcattcc ggtgaacacc gcctcgatcg cgcgataaag
     2761 cagaccgtgc ttgccggaat cgtggtgcag gaagcgcgag cacatcatgg gcgtcagcgt
     2821 caggcacacg aaggcggaga ccatcactgc cacggtcacc gtcaccgcga actcgcggaa
     2881 caggcgtccg acaatgcccc ccatcagcag cagggggatg aacaccgcga ccagagagaa
     2941 gctgatcgac acgatggtga agccgatttc gcccgcgccc ttgatggcgg cctccatcgg
     3001 tttcatgccc tcttccacgt gacggaaaat gttctccagc atcacgatgg catcgtcgat
     3061 aacgaagccc accgcgatgg tcagggccat cagcgacaga ttgtccaggc tgtaattcag
     3121 cagatacatg aacgcgaagg tgcagataag agccactggc accgtcgtgg ttgggatgat
     3181 cgtcgcccag aggttccgca ggaacaggaa gatgaccatc acgacaaggc agacggcgag
     3241 catcaaggtg aactgcacgt ccttcaccga ggcgcggatg gtcaccgtgc gatccatgat
     3301 ggtcgtgatg tggatcgccg gtgggatcga tgcttccagc ctgggcagcg ctgtcttgat
     3361 ccgttccacg gtgtcgatca cgttggcgcc gggctgcttg aagatgatca ggaggatgcc
     3421 gcgcttcccg ttctgccacg cggcgagcat ccggttttct ggcccatcga tcgcatggcc
     3481 gatgtcgctc acgcggattg gcgcgccatt gcgataggcg aggaccacgt ccttgtactg
     3541 gtcagcgcgc gtgagctggt cgttggcgta gaccgtgaat gcctgatgct cgccatcgat
     3601 gctgcccttc ggcgcattga ccgtcgcgct ttggagcagg gtgcggacgt cctccagcgt
     3661 catgcccttc gcggcgatct tctccggatc gacctcgatc cgcaccgccc gcttctgctg
     3721 tccgccaatc agcacctgtg cgacgccgga tatctgcgag atctgttgcg acagcacgtt
     3781 ctcggcataa tcgtccaggt cgatcagcgg catcgcctcg gactgtgccg cgaggatcat
     3841 gattggcgcg tcggacgggt tcaccttccg ataggtcggc ggacccggca tggtcttcgg
     3901 caattgcccg gccgcatcct ggatcgccgc ctggatatcg cctgccgcgc cgtcgatgct
     3961 gcggtccagg tcgaactgca cggtcacgct cgtcacaccg aggatgctgg acgaggtaag
     4021 ctgcgaaaca ccagggatct gggcgatctg ggtctccagc ggctgcgcca ctgtcgccgc
     4081 catggtttcc ggacttgcgc ctggtaactg ccccgtcacc acaatggtcg ggaagtcgac
     4141 gcgcggcagc ggcgcgaccg gcagcagcgg ataggcggca atgcccacca gcaccagcgc
     4201 ggccatcacc agcgatgtcg cgatcggccg tttgatgaag ggggtggaga tgctcatgcc
     4261 gcgttcagcc gccggtctgc gccggtgacg cggacgccgg ggcggatggc gtcatcgccg
     4321 tgatcagcat gccgttgtcg agccgggact gtccgtcgag cacgatctcc tgcccggcct
     4381 gcaagccctt cgtgatgacc atcacgtcgc cgcggtccac cgcctccacg ctctgtcgca
     4441 gtaccgtgga gtccggcttg accatgtata cgtagaggtt ggccatcccg tgcagcacgg
     4501 cctggcttgg cacggtcacc acctgaggct cggtgcgcac cagaacccgc gcttcgacga
     4561 attgtcctgg ccacaaccga tcattttcgt tggcgaaggt agctttcaac ttcaccgtgc
     4621 cagtggccgg atcgatcatg ttgtctggcg tcagcagcga gccttcgctc aactgctcgt
     4681 gctcatcgtt cgaccatgcg gtgaccggca gggtgccttt gcccatcgcc tcgacgatct
     4741 ccggcagttg gcgttgcggc agcgtgaaca cgacggcgat tggacgaacc tgggtgatca
     4801 tcatgatgcc ggcaaggtcg gttgcacgca cgatattgcc aggatcgagc tgtcgcaggc
     4861 cgatgcgccc atcgaagggc gccgtgatgt aacagaagct gaggttcagc tgcgcggtct
     4921 cgatcgccgc gtcgtcgcct ttcagggatg cggtgagctg ggccacctgc atcgtctggg
     4981 tatcgacctg ctggcgcgag gcgaaactcg actgcgccag cgaagcgtag cgctgcaggt
     5041 cgagcttcgc gttgccgagc tgggcctgat ccgccccctt ctttgcctgc gcctggtcca
     5101 gcgatgcctg gtatggccgc ggatcgatca ctgccagcag cgcacccttc ttgaccaact
     5161 ggccctcggt taccggcacc tgcatcagtg tgccgtccac ccgggcgcgc acgaccacgg
     5221 tgttcagcgc ctgcacggtg ccgatgccgc gcagccagac cggcacgtcc tgccgcttgg
     5281 ccttggtcac gctgacggga accgcggtgg ccgaaggacg cgcctgctgc gccagcgccg
     5341 gaacacatgc ggcccagccc aggaagacca gcatcacgaa attcgcaatt agtccacggc
     5401 gtattggcgc cgagaatacc gccttacgtc caggtcgcac tcaagtctcc cgaagcccct
     5461 gtcgcgtgcc ccatgtcgtc cagggcagcg ccattccaat ttttgcattc tccgtgaaat
     5521 tgtcgcgaac tgccagcggc aaatcccgtc gctgactaaa gcttaacccg ccgaaacaca
     5581 ctgaaacatg cgtcagtccg ctgagacgcc gagggcgcgt ctgaaccggt cggccaggta
     5641 gcccccgtcg cgtggaggga gatgtcgcgg tggttcatcc ggcgcaatcc gcagcaccgc
     5701 gaacagatgt tcctgccgga gccggatgcg gagtgcctcc acttcgtcca tgctgcgcgc
     5761 ggtggttgta tttgaccagc cacaggccgc ggcaaccgcc gcgaggtccg tcgtcaaccc
     5821 ggtatggctg ggctgcgaac ctgtctcgcc gtagcgcgca ttgtccagca ccgcgatcgc
     5881 gaggttcgga gcccttgcag cggcaatggt tgcaagcgat cccattccca tgagcatctc
     5941 gccgtcacct gtcagcacca gcacgcgccg cgagggctgc gccagggcga tccccagccc
     6001 gatcatcgca gcaccaccca tcgcacccca caggtagaaa ttgagcggcc ggtccccggc
     6061 agccgctgcg tcataactgg gcgctcccag cccgctcacc acgagcagat cgcctggatc
     6121 gcgcagcagg acggaggcga ccttcctccg gtcaagcgtt ccgtcgtttc tcatgtgtag
     6181 ttccgggcgc gagcccggcc tcttgagcct gtcaccgggc ttgtcccgat ggtcattgcc
     6241 ggacttgatc cggtgatcac ttcacgaaca ccttcgcgcc gatcagccgc tgcgagaaca
     6301 gcacggcgac cggcacggca ctgtcgaatg cgaatgctgc cgaggccgcg acgatctccg
     6361 ccacatcgcc tggctgatcg gctcggcgca catgcacgcc ggcggcggtc agcacggccg
     6421 gtgtggcagt gcccatcggc acctgccacg gattgaactc cgcccattcg ccccgcatgg
     6481 tcaccagcgt caggaacggc atccggcaat tgaccggcag cgacagcatg ttgatgcagt
     6541 tccccacacc cgatgactgc atcagcagca ccgcccgctg gccaccgatc cacgcgcctg
     6601 cggcgagggc gatgccttcc tcctcggtgg tcagcacgac ggtgtgcatc tccggatcgg
     6661 cgtgcgccga cgcaatcaaa tgcgcatgcc ccgcatcggg cacgtagcca acctgcgtaa
     6721 tgcgcgctgc cttcagcgcg gcaaacacct ctccctgcca ggattgagcg ggcatgctgg
     6781 tcctattcgt gtgcttccgg gatgccaaat tggcgcctgg cgggaacggc gccaaccgtc
     6841 ccccgccagt accgccaaag cgctcaggct gccgcgaact gtccgcgccc gaacatcttg
     6901 tccagatcgt ttgcctgtgc cttgtcgaac tgcgtcgtcc tggcacggac atcttcgacc
     6961 gcagcgagtg ccttcttcac cgcggggcgc tcgttgatca cccccaccca ccgcatcagg
     7021 ttcggatact tcgcggctgc atcgttgccc agcaatgccg ggatgttccg tgcccacgga
     7081 taggtcgcga tatcggcgat gccatattcc gcaccgccta accaatgcgt gctcgccagg
     7141 cgctggtcca tcacctcgag cacgcgatgc acctgtgtcg tgtagcggct cttggaatag
     7201 tcattccccg ccggcgcgaa gcgcacgaaa tgcacatgct gcccgaacat cggtccgagc
     7261 gtcgtcatct gcaccatcat ccactcgatc gcctcgtatt tcgccgccat gtcctttggc
     7321 agcagcttgc cggtcttctc ggcgaggtat tgcaggatgg cgcccgactc gaacagcgtg
     7381 atcggcttgc cgcctggtcc gtcgctgtcg gtgatcacgg gcaccttcgc catcgggttg
     7441 agcttcagga actgcgcgtc gaattgcttg ccggtgaaga cgtccacggg atgaacgttg
     7501 tacgacagtc caagctcctc gagcgcaatg tagatcttca ccacattcgg gctgcccatg
     7561 ccgtaaagtt cgatcattgg ttcctcctgg tctgatgtcc caatttcgca cgcccggtcg
     7621 ccaggagaaa ggacggtgcg accgaatgcc gcttgccatt gccgcactgc ggcattgcgc
     7681 ctaggctggc gtgaaattcg aaaggctcgc cgccgggtgg gagccacggt ttacgccatc
     7741 caccaccgtc gaaaggtcag cggtgcagag cgaggcgttc gtattcgggt cgttcacgct
     7801 gatgccggcg gagcgcacgt tgctcgacgg tgacatgccg ctgcggctcg gcagccgcgc
     7861 actcgatatc ctggtcgcgc tggtggaaaa cgcaggcgaa accgtccgca aggacgaact
     7921 gatggcccgt gcctggcccg acacggtcgt tgatgaagcg tcgttgcgcg tgcatatcgc
     7981 ggcgcttcgc aagaccctgg gggatggcca cgccggcagg cggttcatcg ccaacatccc
     8041 gggccgcggc tacgtgttcg ttgccgtggc cactcgccag cacaacgcgg tcatcgagcc
     8101 gcctcgcgct gcgataaccg gcagcaacat cccggcctcg ctcacccgga tcatcggccg
     8161 cgacaatgtc atcgcgatgc tctccgaaca gatcgccagg cgacggctgc tgacgatcgt
     8221 gggcccaggc ggcatcggca agacgacgat agccgccgcc gtggctgagg cggcccgccc
     8281 gtcattcccg gatgggctgt ggtatgtcgg cctggcatcc gtgcccagtg cggaactggt
     8341 gccaacggcg gttgccgcgg tgctcggtat ctcgctccct gccaccgatc ccatcgcggg
     8401 tctcgtggca tggctgcgcg acaggcaggc gctcatcatc ctcgataatt gcgagcacgt
     8461 tgtcgaagct gccgcggcac tggccgagag cattgtccgg tctacgccaa acgtccggct
     8521 gcttgcgaca agccgcgaac cgctgcgtgc tgtgggcgag tggcggcatc gccttacgcc
     8581 gcttgagttc ccgccccatg cgaacggatt ggcggcggag gacgcactgc aatatcccgc
     8641 gattcaattg ttcaatgagc gtgccgtcgc cacggcggac gagtttctac tgaaggacgc
     8701 cgacgttccc gcgctggtgg aaatctgccg gcgcctcgac ggtgtaccgc tggcgctgga
     8761 actcgccgcc gcgcatgtcg gcgtgctcgg catcaaaggg cttgcggagc ggcttgacga
     8821 tcggctcgcg ctgctgatcc aggggcgccg gacggcgctg ccgcgccatc agacgctacg
     8881 cgcaacgctc gactggagct atgacctgtt gcctgcggac gagcaggtca tcctgcgccg
     8941 ccttgccgcc ttccagggcg agttcaccat ggaagccgcc agcgcggtgg ccaccgatgc
     9001 ggcaatcagt tcggatgatg tggtgaacgc cattgccagc ctggtcgaca agtcgctggt
     9061 ctcggccgac atcggcggcg acgttaccta ctaccatctg ctggaaatga cccgcagcta
     9121 cgcgcaggag cggctggcgg gcggtggtga gcgcgatcgg atcatgcgcc ggcacgcgga
     9181 gtatttccgt gaggtgtttg cggcgtccca tgccggtgcg gacgtgcggc cgaaggagga
     9241 gtggctgcgc cactatgggc ggcacatcgg caatctccgc gccgcgctgg actgggcatt
     9301 cgggcggggc ggcgacgttg ctcttggcgt tgtgcttgcc gctgctgcaa ctgatttctg
     9361 gtgtgcgctc gcactgctga gcgaatgctg cgactgggga acgaaggcga tcgcacagct
     9421 cggcgccgcc gaaggaacgc gcgacgagat gcggctgcaa tgcagccttg ggcagtccta
     9481 tacctacagc aagggcatgc gcctggatgc caagtcggcg ctgaaccgtg cggcggtgct
     9541 tgccgaggag ctgggtgagc tgcagtacca ggttcgtatc tactatgcc
//
LOCUS       DATGEM010000331         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1203732, whole genome shotgun sequence.
ACCESSION   DATGEM010000331 DATGEM010000000
VERSION     DATGEM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203732"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>2823
                     /locus_tag="VKQ27_10605"
     CDS             45..>2823
                     /locus_tag="VKQ27_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY89425.1"
                     /translation="MRFTHRFIDRPILATVISVFLMLIGLGALVILPVAQYPEIVPPT
                     VQITTVYPGASADVVSKTVATPLEQEINGVEDMIYMSSQSTGDGKLTLTVTFRIGTDL
                     NVAQMLTQNRVQDALPRLPEDVQRLGVQVRKATPNILLAVHLYSPDGTRDNLYLSNYA
                     TLHVKDALARLPGVGDVQLFGARDYAMRIWLDPDKVAAYNLNADEVLAALRAQNVQVS
                     AGILNQPPVAGTPAYQLNVQALGRLTTPDQFADIVLKNDGQGRVTRLRDVGRVEVGAA
                     DYGSTAYMDRAEGLPLLIFAQPGANSLAVEHEVLSTMQDLKKDFPPGVSDIIIYDPTI
                     FVAKSVHEVVTTIFVAILLVVGVVFLFLQTWRASLIPVIAIPVSLVGTFIILYALGIS
                     LNNLSLFGLVLAVGIVVDDAIVVVENVERNIAAGMTPKEAAHRTMDEVGGALVSIALV
                     LCAVFVPSAFLSGISGQFFRQFAVTIAASTVISCFVSLTLSPALCSLLLLPHAPHEAR
                     RRSLVARLLHGAFGYFNRGFERMSDAYGALTSRLVRALAVVVVVYVGLIATAGVEFSR
                     EPTGFIPEQDQGYLITVLQLPPGASLQRTEQVVKRAVDIILKTPGVEHVAPFAGLDAT
                     TFTVASNAGTIFSGLPSLYNHDIPGITANSVLADLRKRLSVIQDAYVLTIPPPPVQGI
                     GNSGGFKMMLEDRGGLGTEALVKAANTLVAAANKDPSFAGVFTLINAGSPSVYADIDR
                     LKAEKVGLTPTDVFNALQVYLGSQYVNDFNFLGRTYEVIAQADARFRRDSQDLTGLKA
                     RNSAGEMVPIGTVAHLKDATIPYRVPRYNLYPAAEVQGVAAPGVATGTALHRMEALAA
                     TVLPAGIGYEWTEIAFQQEQKGTPTMLVFGAAALFVFLVLAANYESWKLPLAVVLIVP
                     MCLLASVTGLMF"
BASE COUNT          479 a          914 c          859 g          571 t
ORIGIN      
        1 gatcacgccg gctccgtccg gcgccgcaag ctaggacgct caccatgcgt ttcacgcacc
       61 gcttcataga ccgccccatt ctggcgacgg tgatcagcgt cttcctgatg ctgatcgggc
      121 ttggcgctct ggtgatcctg ccggttgcgc aatatccgga gatcgtcccc cccaccgtcc
      181 agatcacgac ggtctatccc ggcgcctccg ccgacgtggt cagcaagacc gttgccacgc
      241 cgctcgagca ggagatcaac ggcgtcgagg acatgatcta catgagcagc cagtcgacgg
      301 gcgacggcaa gctcacccta acagtgacat tccgcatcgg cactgatctg aacgtggctc
      361 agatgctgac gcagaaccgc gtacaggatg cactgccgcg gctgccggag gacgtccagc
      421 gccttggcgt gcaggttcgc aaggcgacgc cgaacattct gcttgccgtg catctctatt
      481 cacccgatgg aacgcgcgac aacctctacc tgtcgaacta tgcgacgctg catgtgaagg
      541 atgcgctggc gcggcttccc ggtgtcggcg atgtgcagct tttcggcgcc cgcgattacg
      601 cgatgcgcat ctggctcgac cccgacaagg tggccgctta caacctgaat gccgacgaag
      661 tcctcgcggc tctgcgcgcg cagaacgtgc aggtctcggc gggcatcctc aaccagccgc
      721 cggttgccgg gactccggcg taccagctca atgtgcaggc gctcggccgg ctcaccacgc
      781 ctgatcagtt cgccgacatc gttctgaaga acgatgggca aggacgtgtg acccgtctgc
      841 gcgatgtcgg ccgcgtcgag gtcggcgcgg ccgattacgg gtccacagcc tacatggatc
      901 gcgccgaggg cctaccattg ctgatcttcg cgcagcctgg cgcgaactct ctggcagttg
      961 agcacgaggt cctgtcaacg atgcaggacc tcaagaagga ctttccccca ggcgtcagcg
     1021 acatcatcat ctacgacccg acaatcttcg tcgcgaagtc cgtgcacgag gtggtgacga
     1081 cgatattcgt cgcgatcctg ctcgtcgtcg gcgtggtgtt tctgttcctg caaacctggc
     1141 gtgcctcgct gataccggtg attgccattc cggtctcgct tgtcggcacc ttcatcatcc
     1201 tttacgcgct cggcatatcg ctgaacaatc tttcgttgtt tggactggtg cttgccgtcg
     1261 gcatcgtggt cgacgatgcg atcgtcgtgg ttgagaacgt cgaacgtaac atcgccgctg
     1321 gcatgacacc gaaggaagcc gcgcatcgca cgatggatga agtaggcggc gcccttgtct
     1381 ccatcgcgct ggtgctgtgc gcagtgttcg taccatccgc ctttctctcc ggcatctcgg
     1441 gccagttctt ccgccagttc gccgtcacca tcgccgcttc gacggtgatc tcctgcttcg
     1501 tgtcgctgac cctgagtccc gcgctctgct cgctgttatt gctgccacat gccccgcacg
     1561 aagccaggcg acgttcgttg gtggcgcggc tgctgcatgg cgcgttcggc tacttcaacc
     1621 gcggcttcga gcgcatgtcg gacgcctatg gcgcgctgac aagccgcctg gtgcgggcgc
     1681 ttgcggtcgt cgtggtggtc tatgtcggcc ttattgccac agctggcgtc gaattcagcc
     1741 gcgagccgac cggcttcatc cccgagcagg atcagggcta tctcatcacc gtcctgcagc
     1801 ttccgcccgg tgcgagcctg caacgcaccg agcaggtagt gaagcgcgcc gtcgacatca
     1861 tcctgaaaac gcccggcgtt gagcatgtcg ctccgttcgc aggtctcgat gcgaccactt
     1921 tcaccgtggc ttccaacgca ggaacgatct tctccggctt accgtcgctg tacaaccacg
     1981 acatccctgg catcactgcg aacagcgtgc tggccgatct gcgcaagcgg ctttcagtga
     2041 tccaggatgc ttacgtgctg acgatcccgc cgccgcccgt gcagggcatc ggcaattccg
     2101 gcgggttcaa gatgatgctg gaggatcgcg gcggcctcgg taccgaggca ctcgtcaaag
     2161 cggcgaacac gctcgtggcc gctgcgaaca aggaccccag cttcgccggc gtatttacgc
     2221 tgatcaatgc ggggtcgccg tcggtctatg ctgatatcga tcggctcaag gccgagaaag
     2281 tcgggctgac accgaccgac gtattcaacg cgctccaggt ctatctgggc tcgcaatacg
     2341 tcaatgactt caatttcctt ggccgcacct acgaggtgat cgcccaggcg gatgcgcgct
     2401 ttcgtcgcga cagccaggat ctgactgggc tgaaggcgcg caacagtgcc ggcgagatgg
     2461 tgccgatcgg aacggtggct catctgaagg acgcaacgat cccctaccgc gtgccacgct
     2521 acaatttgta tccggcggcc gaggtgcagg gcgtcgccgc gccaggcgtt gcgacgggca
     2581 ccgcactgca tcggatggag gcgctcgcgg caacggtact tcctgcgggc atcggctacg
     2641 agtggaccga gatcgccttc cagcaggaac agaagggcac gccgacgatg ctggtgttcg
     2701 gtgctgccgc gctgttcgtg ttcctggtgc tggcggcgaa ctacgagagc tggaagctgc
     2761 cgctcgcggt cgtgttgatc gtgccgatgt gtctccttgc ctccgtcaca ggactgatgt
     2821 tcc
//
LOCUS       DATGEM010000332         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1392815, whole genome shotgun sequence.
ACCESSION   DATGEM010000332 DATGEM010000000
VERSION     DATGEM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392815"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..498)
                     /gene="acpS"
                     /locus_tag="VKQ27_10610"
     CDS             complement(91..498)
                     /gene="acpS"
                     /locus_tag="VKQ27_10610"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HLY89426.1"
                     /translation="MILGLGSDLCDIRRIEAAIERHGDRFINRVFTETERAKAASRTE
                     RIQAATYAKRFAAKEAAAKALGTGFRNGVFFSNLGVANLRSGQPTLQMTGGAATRLAA
                     ITPAGMVASIALTMTDEYPYAFAQVIIWAMPER"
     gene            complement(583..2829)
                     /locus_tag="VKQ27_10615"
     CDS             complement(583..2829)
                     /locus_tag="VKQ27_10615"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HLY89427.1"
                     /translation="MTGGGPVFAPEGVAAEAVHAPSPPAITLCDKLRAYDPKADTGVI
                     DAAYTLAAHAHGTQLRDNGDPYITHPVAVANILAGYRLDTASIVTGLLHDVIEDTPVK
                     LAELQTRFGGEIAGLVDGVTKLTRLELQSDRTKQAENFRKLVLAISRDIRVLLVKLAD
                     RLHNMRTLHFVQDPERRRRIARETMEIYAPLAERIGMDAVKTELQNLAFAQLDPEACA
                     TIQARLNFLRGQGADVIEEVRAELKRVCEQGGVNVVEVVGREKSPYSIWEKMHRRNVA
                     FEQLSDIMAFRIVVPSKEACYAALGAVHSAYPVIAGRFKDYISTPKSNGYQSLHTGVT
                     LREPRNQKIEIQIRTADMNDIAENGVAAHWVYKGPDKKKADKNDLQRFRWVQDLLEIL
                     ENSAAPDEFLENTKLELYEDQVFCFTPKGQLIQLPRGATPVDFAYAVHSQVGDACVGA
                     KINGRLLPLRHQLQNGDQVEIMTARGGTPSPQWERFVVTGKARARIRRFIHQQQRQQH
                     IDMGRAELTKAFRGAGVDGSEKALEPALKTLKFASVDDLYVAVGNGNLGPKDVVHAAY
                     PELRQAPRAPRIIPAPPTRARHGHKTDFDMPITGLVPGMAYHFAGCCHPVPGDEIVGI
                     VTTGKGVTIHARDCPTLASFAATPERFIEVDWNQDALSGGRHDGKALHHTARISVIGS
                     NEPTALANLTNAIAKQDGAVASLKIVNRQADFIEALVDVDVRDVAHLSKVIAGLRGAV
                     GIRGVERG"
     gene            complement(2836..3246)
                     /gene="rpoZ"
                     /locus_tag="VKQ27_10620"
     CDS             complement(2836..3246)
                     /gene="rpoZ"
                     /locus_tag="VKQ27_10620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLY89428.1"
                     /translation="MARVTVEDCVQKVPNRFELVLLAAQRARNLSRGEELTVDRDNDK
                     NPVVALREIAEETIELGKIEQDLVKSLSRAPEPEPADEEVLDLIPTDQNIFGLQDVTA
                     EEEAATLPVEGEDMSPEDLEAAIEAELGGRAAPR"
     gene            3363..3800
                     /locus_tag="VKQ27_10625"
     CDS             3363..3800
                     /locus_tag="VKQ27_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89429.1"
                     /translation="MMTTRVHTLGGLALFFGWLLAGAAGCAPPAPPSPAFDGMYVGQD
                     SLVRGGGFLCGLPTRDLSIAIRNGQFDYPFQVVPPRTAPMPVRVAADGTVAGQLEYGT
                     SESAFRREYITAQATLSGRITGDMIEATISDLRCTRRLVARRS"
     gene            complement(3936..4445)
                     /gene="folK"
                     /locus_tag="VKQ27_10630"
     CDS             complement(3936..4445)
                     /gene="folK"
                     /locus_tag="VKQ27_10630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLY89430.1"
                     /translation="MILVGIGSNLPGQDGVAPLETCRWAVRRLAALPGLRLDAVSQWY
                     VTRPMPPSGQPPYVNAVARLAVDPDAAEPDPAVLLAALHAIEAEAGRVRGEPNAARTL
                     DLDIVAMGGMVRTAPDPVLPHPRAHLRAFVLVPLLDVAAGWIHPVEGVEAGELLRRLP
                     DQGIRLLQR"
     gene            4587..5192
                     /locus_tag="VKQ27_10635"
     CDS             4587..5192
                     /locus_tag="VKQ27_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLY89431.1"
                     /translation="MHFLPNERVALFIDGANLYSASRNLGFDVDYRNLLDFFRKKSHV
                     IRAYYYSAVLETDEYSPLKPLTDWLAYNGYTLVTKPAKEFTDSSGRRRVKGSMDIEVA
                     VDMLELAPRLDHAILFSGDSDFRRLVEAVQRKGVKVSVVSSIRTTPPMVADELRRQAD
                     HFLELAEIAPEFTRRQTEPRARSVPVRQTPAEPFVDPHDAA"
     gene            5189..5824
                     /locus_tag="VKQ27_10640"
     CDS             5189..5824
                     /locus_tag="VKQ27_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLY89432.1"
                     /translation="MTTGAPAPDCPLCPRLCEYRQANRAAHPDWFNGPVPSFGSLDAR
                     LLIVGLAPGVRGANRTGRPFTGDFAGELLYRTLLHFGLAHGHYRADPQDGLALVNCRV
                     TNALRCVPPANLPTPLETANCNQFLAAEIAAMPDLRAVLALGGTAHNAVLRANGLRPA
                     RIAFKHGALHTLPGERVLADSYHVSRLNTNTGRLTEAMFHDVVRDLLERMV"
     gene            5897..6376
                     /locus_tag="VKQ27_10645"
     CDS             5897..6376
                     /locus_tag="VKQ27_10645"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HLY89433.1"
                     /translation="MDFRITGLPVEPFIPFFNMSDEELLTHGASRVVAKPEDAPLMQP
                     CRVSLRDSEPADVSILLNFPHHVTPTSPYRASGPIFVREGVTETASFINHVPAQQRIR
                     LLSVRAYDAAGIMVDADVREGTDLEAVIERMFARDDVAFLHAHNAKRGCYSCRIDRV"
     gene            6386..>6939
                     /locus_tag="VKQ27_10650"
     CDS             6386..>6939
                     /locus_tag="VKQ27_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY89434.1"
                     /translation="MNIYAQTLLDAAYAAFRNRRYAEAFNVLGFVAGSGWRDALTFRF
                     LAHLEDLRDDTEAALSWLSAAIAIDPANPTAHSHSGDALFKLNRMSDAEAAYRRAITL
                     DPTLTEAYGGLAQVLHLADRDDEALACAERSQIDAKDPVHAHRILGTALAALNRHEEA
                     IEQFRTAQIVNPDDATARTYEGMAL"
BASE COUNT         1163 a         2368 c         2102 g         1306 t
ORIGIN      
        1 gtgtagggat catagacgca gccactcagc agcgccgccg caccggcaag cacgacgaca
       61 gcaccaaggc gtcgcatggt ttgctctcct ttaccgctcc ggcatcgccc aaatgatcac
      121 ctgggcgaac gcgtacgggt attcgtcggt catggtcagc gcaatcgagg cgaccatgcc
      181 agcgggcgtg atagccgcca gccgcgtcgc cgcgccacca gtcatttgta acgtaggctg
      241 accggagcgc aggttcgcaa caccgagatt ggagaagaac acgccattcc ggaatcctgt
      301 gcccagcgcc tttgccgccg cttcttttgc ggcaaaccgt ttcgcgtaag tcgcggcctg
      361 gatgcgctcg gtgcggctcg ccgccttcgc gcgttctgtt tccgtgaaga cgcggttgat
      421 gaaccggtca ccgtgccgct ctatcgcggc ctcaatacgg cggatgtcgc ataggtcgga
      481 gccgaggccg aggatcatga gcgtgctgtc attgccagca gcggagccgc gggcgggcca
      541 ggccaaggaa cccttgcgcg cctgggaata acggaacagc cctcatccac gttccacgcc
      601 acgaatgcca accgcgccgc gcagacccgc gatcaccttc gacaggtgcg ccacgtcgcg
      661 cacatccaca tcgaccaggg cttcgataaa gtccgcctga cgattgacga tcttcaagct
      721 cgccaccgct ccgtcctgct tggcgatcgc attggtgaga ttggcaagtg ccgttggctc
      781 attcgacccg atgacgctga tgcgcgcggt atgatgcagg gccttgccat catgccggcc
      841 acccgacaac gcatcctgat tccagtccac ttcgatgaac cgttccggcg tggcggcgaa
      901 acttgccagc gtcggacaat cgcgcgcgtg gattgtcacg ccctttccgg tggtgacgat
      961 gccgacaatc tcgtcgcccg gtaccggatg gcagcagccg gcgaagtgat acgccatgcc
     1021 cggcaccagc ccggtgatcg gcatgtcgaa gtcggtcttg tgcccgtgcc tcgcgcgcgt
     1081 cgggggcgcc ggaatgatcc gcggtgcacg cggtgcctgc cgcagctccg gatatgcggc
     1141 atgcaccacg tccttcggcc cgagattgcc gttaccgaca gcaacataaa ggtcgtcgac
     1201 tgatgcaaat ttcaacgttt tcagcgccgg ctccagcgcc ttttccgagc cgtcgacacc
     1261 cgcgccacga aacgccttgg tcagctcggc gcgtcccata tcgatatgct gctgccgttg
     1321 ctgctgatgg atgaaccgcc ggatgcgtgc ccgcgccttg ccggtgacga cgaagcgttc
     1381 ccactgcggc gatggtgtgc cgccgcgcgc cgtcatgatc tcgacctggt cgccgttctg
     1441 cagctggtgc cgcaacggca gcagccgccc gttgatcttc gcgcccacac aggcatcgcc
     1501 tacctgcgag tgcaccgcat aagcgaagtc caccggcgtc gcgccgcgcg gcaactggat
     1561 caactgaccc ttcggcgtga agcagaagac ctggtcttcg tagagttcca gcttggtgtt
     1621 ttccaggaac tcgtccggcg ccgcagaatt ctccaggatt tccaggaggt cctggaccca
     1681 tcggaaccgc tgcaggtcgt tcttgtctgc cttcttcttg tccggtccct tgtagaccca
     1741 gtgcgcggcg acgccgtttt cggcgatgtc gttcatgtcc gcggtgcgga tctggatctc
     1801 gatcttctgg ttgcgcggtt cgcgcagcgt cacgccggtg tgcaggctct ggtagccgtt
     1861 cgatttcggc gtggaaatgt agtccttgaa ccgccccgcg atcaccggat acgccgaatg
     1921 caccgcgccg agcgcggcgt agcaggcctc cttgcttggc acgacgatgc ggaacgccat
     1981 gatgtcggac aactgctcga atgcgacgtt gcggcggtgc atcttctccc agatcgagta
     2041 gggcgacttc tcccggccca ccacctcgac cacgttcacg ccgccctgtt cgcaaacgcg
     2101 cttcagctcc gcgcgcacct cctcgatcac atccgcgccc tggccacgca ggaagttcag
     2161 ccgcgcctgg atcgttgcgc aggcttccgg atcgagctgt gcaaacgcga ggttctgcaa
     2221 ctcggtcttc accgcatcca tgccgatgcg ttcggcgagg ggtgcgtaga tttccattgt
     2281 ttcgcgcgcg atgcggcggc ggcgctcggg atcctgcacg aaatgcaggg tcctcatgtt
     2341 gtgcaggcga tcggccagct tcaccagcag cacgcggata tcgcggctga tcgccagcac
     2401 gagcttgcgg aaattctccg cctgcttggt gcggtcggat tgcagttcca ggcgggtcag
     2461 cttggtgacg ccgtccacca gccctgcaat ctcgccgccg aacctggttt gcagctccgc
     2521 cagcttgacc ggcgtgtcct cgatcacgtc gtgcaacagc cccgtgacga tcgaggcggt
     2581 atccagccgg taacccgcca ggatgttcgc aaccgctaca gggtgggtga tgtagggatc
     2641 gccgttgtcg cgaagctggg ttccgtgcgc gtgcgccgcc agtgtgtagg ctgcgtcgat
     2701 cacgccggta tcggccttcg gatcgtaggc tcgcagcttg tcgcagagcg tgatcgcagg
     2761 cggggagggg gcgtgcacgg cttccgccgc cacgccttca ggcgcgaata cggggccgcc
     2821 ccccgtcata tgcggctacc ttggggcggc gcggccgccc agctcagcct cgatggccgc
     2881 ctcgagatcc tcgggcgaca tatcttcgcc ttcgacgggc agggtcgccg cctcttcctc
     2941 ggccgtgacg tcctgcaggc cgaagatgtt ctggtcggtc gggataagat ccaatacctc
     3001 ctcgtccgcg ggctcgggct caggcgcgcg ggacagggat ttcaccaaat cctgctcgat
     3061 cttgccaagc tcgatagtct cctcggcgat ctcgcgcagc gcgacgactg gattcttgtc
     3121 gttgtcgcga tcgacggtca gttcctcgcc gcgactcagg ttgcgcgcac gctgggcggc
     3181 aagcaacacc aactcgaagc ggttcggaac cttttggacg caatcttcaa cggtgacgcg
     3241 cgccatgcgc tcagcctcca ggcattggac ttgggcgaac tggtcagata agcgaggcag
     3301 tgcgtcaaag caagcctcgg agcaggttct tgccctctgg cgcgacggta caacattcgg
     3361 gcgtgatgac cactcgtgtg cacaccctcg gcgggcttgc gcttttcttc ggctggctcc
     3421 tggccggggc agccggctgc gctccgcctg caccgccgag ccctgccttc gacggtatgt
     3481 atgtcgggca ggacagcctt gtgcgtggcg gcggcttcct gtgcggcctg ccgacgcggg
     3541 acctgagcat tgcgatccgg aatggccagt tcgactatcc cttccaggtc gtgccaccgc
     3601 gaaccgcacc aatgcccgtg cgtgtcgcgg ccgacggaac ggtggccggg caacttgagt
     3661 acggcacatc ggaatctgcc ttccggcgtg aatacatcac tgcgcaggca accttatctg
     3721 gtcggatcac gggagatatg atcgaggcga ccatcagcga tctgcggtgc acacgaaggc
     3781 tcgtcgcccg gcggagctaa caccggcagc ctcctagagg gtgatggcca aaggcagaag
     3841 tgccggaggc ctgaatcacg cgataaaaca aaaggccaga gcgagatcag cgcgcccttc
     3901 agcgctcatc ccgctctggt ggctgcttcg ccggatcagc gctgaagcag ccgtatcccc
     3961 tgatccggta gccgccgcag cagttccccc gcttccacgc cttccaccgg atggatccat
     4021 cccgccgcga catccagcag tggcaccagg acgaatgccc gcagatgcgc gcgcggatgc
     4081 ggcagcaccg gatcgggcgc cgttcgaacc atgccgccca tggcaacgat atcgaggtcc
     4141 agcgtccgcg ctgcgttcgg ctcgccgcgg acccgccctg cctctgcttc gatcgcatgc
     4201 aacgcggcca gcagtactgc cgggtccggt tccgccgcgt ccgggtcaac cgccagccgc
     4261 gccaccgcgt tcacgtaagg aggctggccg gacggcggca tcggccttgt aacgtaccac
     4321 tgcgagacag catccaggcg cagtcccggc aatgctgcca ggcgccttac cgcccaccgg
     4381 caggtctcca gcggcgcaac accgtcctgt cccggtagat tcgacccgat acccacgagg
     4441 atcattggca atggattccg atcgcctgtt cacaattcgg caagaaactg cgatgatttg
     4501 ctacgcgacc gcctcggttt cctgcctttt cgcggtctgt tcggtttgta tctggctcgc
     4561 acatctgctt cgagaggctc ggttccatgc atttcctgcc caatgaaaga gttgccctgt
     4621 ttattgacgg cgccaatctc tattccgcct cgcgcaacct cggcttcgat gtggactatc
     4681 gcaacctgct ggacttcttc cgcaagaagt cgcatgtcat tcgggcctat tattattccg
     4741 ctgtgttgga gaccgatgaa tactcgccgc taaagccgct gactgattgg ctggcctaca
     4801 atggctacac gctggtgacc aagcccgcca aggaattcac cgattcatcc ggccgccgcc
     4861 gcgtgaaagg cagcatggac atcgaggtgg cggtcgacat gctggaactg gcgccccgcc
     4921 tggatcacgc gatcttgttc agcggagact cggacttccg ccgcctggtc gaggcggttc
     4981 agcggaaggg cgtcaaggtt tcggtcgtct cctcgatccg caccactcca ccgatggtcg
     5041 ccgacgaact gcgccgccag gcagaccatt tcctcgagct ggccgagatc gcgccggagt
     5101 tcacgcgccg ccagaccgag ccgcgtgccc gttccgtgcc ggtgcgccag acccccgccg
     5161 aaccgttcgt cgatccgcac gacgcggcgt gaccacaggc gctccggcgc ccgactgccc
     5221 tctctgcccg cgactgtgcg agtaccggca ggcgaaccgc gccgcccatc ccgactggtt
     5281 caacggtcct gtccccagct tcggcagcct cgatgcgcgt ctgttgatcg tcggtcttgc
     5341 ccctggcgtg cgaggtgcca accgcaccgg ccggccgttc accggcgact ttgccggcga
     5401 actgctctac cgcaccctgc tgcatttcgg cctggcgcat ggccactatc gcgcggatcc
     5461 gcaggacggt ctggcgctgg tcaattgccg tgtcaccaac gcgttgcgct gcgtgccgcc
     5521 cgccaacctg ccgacgccgc tggagaccgc caactgcaac cagttcctcg ctgccgagat
     5581 cgcggcgatg cctgatctgc gtgctgtgct ggcgcttggc ggcaccgcgc ataatgctgt
     5641 gctgcgcgcc aacggcctgc gcccggcacg catcgccttc aagcatggcg cgctccacac
     5701 gctgccgggc gaacgcgtgc tggccgacag ctatcacgta tcccgcctca acacgaacac
     5761 cggccggctc accgaggcaa tgttccacga cgtcgtccgc gatctgctgg agcgcatggt
     5821 ctgagcgcaa tcgccggatg gcgccgctat cccgcatcga cgcatgttgg ctgcgtcaaa
     5881 cgcaaggaga gccaacatgg acttccgcat cacaggcctg ccggttgaac ctttcatccc
     5941 cttcttcaac atgagcgacg aggaattgct gacccacggc gcgagccgtg tcgttgccaa
     6001 gcccgaggac gcgcccctga tgcagccttg ccgcgtcagc ctgcgtgatt ccgagcctgc
     6061 cgacgtctcg atcctgctga acttcccgca ccatgtgacg cccacttcgc cctatcgcgc
     6121 gagcggcccg atcttcgtcc gcgagggcgt caccgagacc gccagcttca tcaaccacgt
     6181 gcctgcgcag cagcgcatcc gcctgctgtc ggtgcgcgcc tatgatgcgg ccggcatcat
     6241 ggtagatgcc gacgtgcgcg aaggcaccga cctggaagcg gtgatcgagc gcatgttcgc
     6301 ccgcgatgac gttgccttcc tgcatgcgca caatgccaag cgcggctgct attcctgccg
     6361 catcgatcgg gtctaaagcg tatgcatgaa catctacgcg cagacgctgc ttgatgccgc
     6421 ctacgctgcg ttccggaacc ggcgctatgc cgaggccttc aatgtcctcg gctttgtcgc
     6481 cggctccggc tggcgcgatg cgctgacgtt ccgcttcctc gcgcatctgg aagatctccg
     6541 tgacgacact gaggccgcgc tgtcctggct ttctgccgcc atcgccatcg atcccgccaa
     6601 cccaacggcc cacagccact ccggggatgc gctgttcaaa ctcaatcgca tgagcgatgc
     6661 cgaagccgcg tatcgtcgcg ccataacact cgatcccacg ctcaccgaag catatggcgg
     6721 cctggcacag gtgctgcatc tggcggatcg cgatgacgag gcgctggcct gcgcggagag
     6781 atcccagatc gatgcaaagg acccggtcca cgcgcatcgc atccttggca ccgcgctggc
     6841 cgcgctcaac cggcatgagg aagcgatcga gcagtttcgc accgcgcaga tcgtgaaccc
     6901 cgacgatgcg actgcacgga cgtatgaagg catggcgct
//
LOCUS       DATGEM010000333         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1642781, whole genome shotgun sequence.
ACCESSION   DATGEM010000333 DATGEM010000000
VERSION     DATGEM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642781"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2459)
                     /locus_tag="VKQ27_10655"
     CDS             complement(<1..2459)
                     /locus_tag="VKQ27_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY89435.1"
                     /translation="MIVPHLFSPDGSRDQLYLSNYATLHIKDAIARLDGVGAVETFGA
                     RDYAMRIWLDPDKTAAHDLTAGEVVAALRAQNVQVSAGVLAQPPVTQPGAFQLNVETL
                     GRLTAPSQFADIIVKQDKEGRVTHLRDIGRVELGALDYSANGYMDRYHSVPILIYRRP
                     GSNELATAREIRKTMAELAKDFPPGIDYFNQYDATVFISQSVHEVVMTIIGAVVLVVL
                     VVILFLQTWRASIIPIVAIPVSLVGTFAILAALGFSINNLSLFGLVLAVGIVVDDAIV
                     VVENVERNLRLGLSPRDAARLTMNEVGGALIAIALTLCAVFVPSAFIAGISGEFFRQF
                     AVTIAASTLISCFVSLTLSPALCAVLLRPHRAHEASIHHPPLVRAIAASFSAFNRGFE
                     WLSERFGLLTSRLIRLSGVLLVVYAGLTGLTAWQFNRAPTGFIPDLDQGYLITVLQLP
                     PGSSLARNDDVTREVNEILLTTPGVATTAAFAGLDGATFTLASNKGAIFSVFTPFEER
                     EPKGLTAKAILASINQRLAGIEGAFVITVEPPPVNGIGGAGGFKMMLEDRNNAGPAAL
                     EAAARDLIAAANADPRLAAVFTTFSSATPSVFADIERVRAAKLGVPPERVFEAMQVYL
                     GSSYINDFNYLGRTYQVMAQADDRFRMGPADVARFKTRNDAGEMVPIGSIASFRDTTG
                     PYRVPRYNQFPAAEILGSPAPGISSGTALAVMEQLAAERLPPGIGFEWTDLSFEQKRA
                     GNTAIFIFLASVVFVFMVLAAQYESWTQPLAIILIVPMCLLAAVSGLLLRGMSVDILA
                     QIGFVVLVALASKNAILIVEFA"
BASE COUNT          532 a          716 c          810 g          436 t
ORIGIN      
        1 gcgaactcga cgatcaggat ggcgtttttg gacgctaggg cgaccaaaac gacgaatcca
       61 atctgggcaa ggatgtcgac actcatgcca cgcagaagca gccccgatac agctgccagc
      121 aagcacatag gaacgatgag aatgatcgcc agaggctgag tccagctctc atactgcgct
      181 gccagcacca tgaacacgaa gaccaccgaa gcaaggaaaa taaatatcgc cgtgttccca
      241 gcccgtttct gctcgaagga aaggtcagtc cactcgaacc cgatacctgg gggcaaccgc
      301 tccgcggcca actgttccat cacggcaagc gctgttccac tcgatatgcc cggggcgggg
      361 cttcccagga tctcagccgc cgggaattgg ttgtagcggg gcactcgata ggggccggtg
      421 gtgtcacgga aactggcaat ggacccgata ggcaccattt cgccggcatc gttgcgcgtc
      481 ttgaagcggg cgacgtccgc gggccccatg cggaagcgat cgtcggcttg cgccatgacc
      541 tgataggtgc gccccagata attgaagtca ttgatgtagg acgagccgag atagacctgc
      601 atcgcctcaa atacgcgttc gggggggacg cctagtttgg ccgctcttac gcgttcgata
      661 tcggcaaaaa cgctcggcgt agcgctgctg aaagtcgtga aaaccgccgc gaggcgtgga
      721 tctgcatttg cggcggcgat cagatcgcgc gcggccgctt cgagcgcagc tgggccggcg
      781 ttgttgcggt cttccagcat catcttgaag ccaccggcgc cgccgatgcc gtttaccgga
      841 ggtggttcca cggttatgac gaacgcgcct tcgatgcctg cgagccgctg attgatcgac
      901 gccaggatgg ccttggcggt caggcctttc ggctctcgtt cttcgaatgg cgtgaagaca
      961 gaaaagattg cacctttgtt tgaggcaagg gtgaaggttg ccccatcgag gccggcgaag
     1021 gcggctgtcg tcgccacgcc aggggtggtc agcaatatct cgttcacttc gcgggtcacg
     1081 tcatcgttgc gtgccaagga agaccccgga ggaagctgca ggacggtaat cagatagccc
     1141 tgatcgaggt cggggataaa tcccgtcggt gcccggttga attgccaggc cgtcagccca
     1201 gtcaggcctg catagactac caggagaacg ccgctcaggc ggatcaggcg actggtcaag
     1261 aggccgaagc gctccgacag ccattcgaat ccgcggttga aggccgagaa ggacgcggcg
     1321 atcgcgcgga ccagcggagg gtgatggatc gaggcttcgt gagctcggtg cggacgcagc
     1381 agcaccgcgc acaaggcggg actgagagtc agcgagacga agcaggaaat cagggtggag
     1441 gcggcgatgg tgaccgcaaa ctgccggaag aactcgccgg agatgccggc gatgaacgcc
     1501 gaaggaacga agacggcgca aagtgtcagc gcgatggcga tgagcgcgcc gcctacctcg
     1561 ttcatggtaa gcctcgccgc atccctgggc gagaggccga ggcggagatt gcgctccaca
     1621 ttctcgacga cgacgatcgc gtcatccacg acgatcccga ccgccagcac cagaccgaag
     1681 agggacaggt tgttgatgga aaacccgaga gcggccagga tggcgaaggt cccgaccagg
     1741 gaaacgggga tcgccacgat cgggatgatc gatgcccgcc aggtctgcag gaacagaatc
     1801 acgacgagaa cgaccaggac gaccgcgccg atgatggtca tcacgacctc atgcacggac
     1861 tggctgatga atacggtggc gtcgtactga ttgaagtagt cgatgccggg aggaaagtcc
     1921 ttggccaact ccgccatggt tttgcggatc tcgcgcgccg tggccagttc gttggagcca
     1981 ggccgccgat agatcaatat cggaaccgag tggtagcgat ccatatagcc gttggcgctg
     2041 tagtccagag ccccgagctc cacccggccg atatcgcgca ggtgagtgac gcgaccttcc
     2101 ttgtcctgtt tgacgatgat atcggcgaac tgcgaggggg cggtcagacg cccgagggtt
     2161 tccacattga gttggaaagc gcctggttgc gtcacgggcg gctgtgccag cacgccggcg
     2221 gagacctgga cattctgcgc gcgcaatgcg gcgaccactt cgccagcggt cagatcgtgc
     2281 gccgccgtct tgtctgggtc cagccagatg cgcatcgcat agtcccgcgc gccgaatgtc
     2341 tcaacagctc ctactccgtc gagcctggcg atcgcatcct tgatatgaag tgtcgcgtag
     2401 ttcgagaggt agagctgatc gcgcgatccg tccggcgaaa agagatgagg aacgatcacg
     2461 tagtcgctcg ctactttctt gacgcttatg ccga
//
LOCUS       DATGEM010000334         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1394744, whole genome shotgun sequence.
ACCESSION   DATGEM010000334 DATGEM010000000
VERSION     DATGEM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394744"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VKQ27_10660"
     CDS             complement(<1..211)
                     /locus_tag="VKQ27_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89436.1"
                     /translation="MTGLNNEPITSEPKDNVHDLQDHSRRRRPAVLSAGAMAVSTSAA
                     DAHYAGSYQTYCKTFYHTVLVGYDDY"
     gene            complement(408..1985)
                     /locus_tag="VKQ27_10665"
     CDS             complement(408..1985)
                     /locus_tag="VKQ27_10665"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HLY89437.1"
                     /translation="MFDDGSRDVAVPVRLKGIRVERSRQFGDVYMALALWRGIGLEDL
                     CERLLPPGQECISWAKMAAVLVAARFCEPSSELHIAEDWYRRTALCDLLQLGDEEVNK
                     DRLYRGLDHLLVHKAALEAHLAKRCGELFAIQNEVLLYDVTSTYFEGLAEANPQAKRG
                     YSRDHRPDCKQVCIALVVTFDGFPLGYEVFAGNTHDSKTLQTIVDTMEARHGVVGRVW
                     ISDRGMASAENLAWLRETGRRYIIGAPKSELKKFAAELARPDGWRTVHEGVEVKLARH
                     PETDETVILCRSADRRSKERAMHDKFSGRIDAALKRLAARLARSKKRLDQAQVNRQIG
                     RILQQNQRAGARFAIALQPDDCPAGFRLVVTHNDAFDDWAAISEGTYLLRSNIDDWSD
                     AQLWKAYIQLTQAEAAFRIQKDQLNVRPIWHQREDRVQAHILVCFLAFVLWKCLEMWQ
                     SRAGLGNSPRTVLEELARIQAHDVVLPTTTHGQIRLRCVTQPDAAQAALLDRLGIVLP
                     KRMRLAEHELPALARSA"
     gene            2318..>2656
                     /locus_tag="VKQ27_10670"
     CDS             2318..>2656
                     /locus_tag="VKQ27_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLY89438.1"
                     /translation="MRKLGYLFVAPDALGLLVFVGAPMFLALSLGFFEVSGFGGYRFV
                     GLANYRRMLADPLFLQSLRVTLTYVVLIVPSLYAAGLGLALLVQRGGRINGICRSLFF
                     IPQTVSLVVVA"
BASE COUNT          462 a          851 c          834 g          509 t
ORIGIN      
        1 agtagtcgtc atagccgacg agaacggtgt ggtagaaggt cttgcaatag gtctggtagg
       61 agcccgcgta gtgcgcgtcg gcggccgagg tcgagacggc catggcgccg gcggaaagaa
      121 cggcagggcg gcgacggcga gaatggtctt ggagatcatg gacgttgtcc tttggttcag
      181 aggttatcgg ttcgttgttc agtccggtca cgatcggtca tcgatcgttg ggctgctttt
      241 acgggagagc gtctgaacca cggctgagac ggccatttat ccaccgttca ggatgagtaa
      301 actcagcgtg agccgcgcgt tagcccaact tgcgcatttt cgaccatcga ctttaggcaa
      361 atcaatacct tagtggaaaa gtcgccacta catttttgag attggggtca ggcgctgcgt
      421 gcgagcgcgg gcagctcgtg ctcggccagg cgcatgcgct tgggtaggac gatgccgagg
      481 cgatcgagga gcgcggcttg cgcggcatcg ggctgggtga cacaacgcaa acggatttgg
      541 ccatgcgttg tggtcggcag gacaacgtcg tgcgcctgaa tgcgggcaag ctcttcgagg
      601 accgtgcgcg gggagtttcc gagaccggcg cggctctgcc acatctcgag gcacttccac
      661 agaacgaagg cgaggaagca aaccaggatg tgggcttgca cccgatcctc gcgctggtgc
      721 cagatcggcc ggacgttgag ctgatccttc tggatgcgga aggcagcctc ggcttgggtc
      781 aactggatgt aggctttcca gagctgcgcg tcgctccaat cgtcgatatt ggaacgcagg
      841 agataggtgc cttccgagat cgccgcccaa tcgtcgaagg cgtcgttgtg ggtcacgacg
      901 agacggaatc cggccgggca atcatccggc tgcaaggcga tggcgaagcg ggcaccggcg
      961 cgctggttct gttgcaggat gcggccgatc tgccggttga cttgcgcttg atcgagccgc
     1021 ttctttgaac gggcgaggcg ggcggcgagg cgtttgagcg ctgcgtcgat gcggccactg
     1081 aacttgtcgt gcatcgcccg ctccttgcta cgccggtcgg cggagcggca gaggatcacc
     1141 gtctcatccg tctcggggtg ccgggcgagc ttgacctcga caccttcatg gaccgtgcgc
     1201 cagccgtccg ggcgcgcaag ctcggcagcg aacttcttca gctccgactt cggcgccccg
     1261 atgatgtagc gacgaccggt ctcgcgcagc caggcgaggt tctcggcact cgccatgccg
     1321 cggtcgctga tccagacgcg gccgaccacg ccatgccggg cttccatggt gtcgacgatc
     1381 gtctgtaatg tcttcgaatc gtgggtgttg ccggcaaaga cctcgtagcc cagcggaaag
     1441 ccgtcgaagg tcacgacgag ggcgatacac acttgcttgc agtcggggcg atgatcgcgg
     1501 gagtagccgc gcttggcctg cgggttggcc tccgccaagc cctcgaaata ggtgctcgtc
     1561 acgtcataga gcagcacctc gttctggatc gcgaacagtt cgccgcagcg cttcgccaga
     1621 tgcgcttcca gcgcagcctt gtgcaccagc aggtgatcga gcccacgata gagccgatcc
     1681 ttgttgacct cttcgtcgcc aagctgcagc aggtcgcaga gcgccgtgcg acggtaccag
     1741 tcctcggcga tgtgcagttc gctcgacggc tcgcagaacc gcgccgcaac cagcaccgcc
     1801 gccatcttcg cccaggagat acactcctgg cccggcggca gaagccgctc gcacagatct
     1861 tccagtccga ttccgcgcca cagcgccagc gccatataga catcgccaaa ctgccgcgag
     1921 cgctcgacgc ggatgccctt caagcgaacc ggcaccgcca catcccggct gccgtcgtcg
     1981 aacaattgcg cctcttccgg cgccccgatc agatgccgcg cgaggcctcg agcttcgatc
     2041 cgacccgcct cgtcaagctc gccgagatgc gccaccgtct gttgaatgac acggcgcccg
     2101 acccggacgc tgcgcaccaa actccaatag cgatggacct tcccgtcctt cttgcgagtc
     2161 gtgtggcgta gatacatgcc gcgaatcaaa actgactcgc ggcccgccgg aaagacccaa
     2221 ggtcgccact acacgccgaa tccgccctct caccgccagc aacccattga tccgtaaagg
     2281 ccgctgcgac aaaacgactc ccccttggga cggcaaattg cgcaagttgg gctacctctt
     2341 cgtcgctccc gacgcgcttg gcctcctcgt cttcgtcggc gcgccgatgt tccttgcgct
     2401 cagcctcggc ttcttcgagg tgagcggctt tggcggctat cgcttcgtcg ggctcgccaa
     2461 ctaccgccgt atgctcgccg acccgctctt cctgcagagc ttgcgggtta cgctgaccta
     2521 tgtcgtcttg atcgtgccga gcctctatgc cgccggactg ggcctcgccc tcctcgtcca
     2581 gcgcggcggc cgcatcaacg gcatctgtcg cagccttttc ttcatcccgc agaccgtcag
     2641 tctggtcgtc gtcgcg
//
LOCUS       DATGEM010000335         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1214753, whole genome shotgun sequence.
ACCESSION   DATGEM010000335 DATGEM010000000
VERSION     DATGEM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214753"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..261)
                     /locus_tag="VKQ27_10675"
     tRNA            complement(186..261)
                     /locus_tag="VKQ27_10675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Glu,seq:ttc)
     gene            complement(304..2073)
                     /gene="recJ"
                     /locus_tag="VKQ27_10680"
     CDS             complement(304..2073)
                     /gene="recJ"
                     /locus_tag="VKQ27_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HLY89439.1"
                     /translation="MSDCVLGVGRSLSGRRWIWRSGQDRIGLGICQRMGVPEIVGRLL
                     AARGIDIDSASHFLDPTLRALLPDPSVLVDMDVAAERLAHAVQHGETVGVFGDYDVDG
                     ACSAALMCTLLRELGCEVLYHVPDRMMEGYGPNGPALHSLVLRGASLIVCVDCGTAAA
                     EALAFVAGDADIVVLDHHKAEGAPPSIVATVNPNRLDCSSGLSFLCAAAVAFLAAVAM
                     VRTLRRQGYFNARKEPDLIGLLDLVALATICDVMPLTGVNRALVCQGLKVMARRARPG
                     VAALLDVAQSRDRLNAGTCSFALGPRINAAGRISEADLGLRLLLCGDPVEAASLAATL
                     DAVNRQRQEVEAAILEAAMAAAEAQAGKGHAALMISGPAWHPGVVGIVAGRIKERFNR
                     PACVAGIADDMAKGSGRSVAGHDLGAVVIAARQAGILKTGGGHPMAAGFSLLPSRLED
                     FQAFLNERLASAASLPAAADLPVEGTLAVPGATMELAEHLARLAPFGAGNEEPILVLQ
                     RARVVRADRVGREGTAIRAFVEGEGGGPRLKALLFRAREGALAETLLCRDGLPLHLAG
                     YLRAEEWNGVVNPGFIISDAAAA"
     gene            complement(2192..>2478)
                     /locus_tag="VKQ27_10685"
     CDS             complement(2192..>2478)
                     /locus_tag="VKQ27_10685"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HLY89440.1"
                     /translation="GQMQGRLLFENDEQVERVKQMGHRDPKRKFSLEEMAKGDVMFAC
                     TGVTSGTMLRGVKRFGHGAATHSMVMRSKSGTVRFVEAHHNFHTKTWVRG"
BASE COUNT          465 a          931 c          678 g          404 t
ORIGIN      
        1 cccgcggcgc tcacttgatg cttcaggtcc gcaccgccat cgtcaacggc acgttcgacc
       61 agaatcaccg cgacatctgc cggcatcgcc cctcatttcg catggcagca tgatcgcccc
      121 catgtttggg acggtctcat gtctaatttc gaccgggcac ccctcgaaaa acctagcaaa
      181 atcaatggcg tcccgtacgg gattcgaacc cgtgttacca acgtgaaagg ctggtgtcct
      241 gggcctctag acgaacggga caacggtcag ggcaggggtg ggtagcggag cacccccggg
      301 gggtcaagcc gccgccgcgt cgctgataat gaacccggga ttgaccaccc cattccattc
      361 ctcagcccgc agatagccag caagatgcag cggcaatccg tcgcggcaca gcagtgtctc
      421 cgccagcgcc ccctcgcgcg cgcggaacag cagcgccttc agccggggcc ctccgccttc
      481 gccttccaca aacgcccgga tcgcagttcc ttcgcgcccg acccgatcgg cgcgcaccac
      541 ccgcgcgcgc tgcagtacca ggattggctc ctcgttgccc gctccgaacg gcgccagtcg
      601 cgccaggtgt tccgccagtt ccatcgtggc tcccgggacc gcgagggtcc cttccaccgg
      661 cagatcggcc gcagccggca aggacgccgc cgacgccagt cgctcattca ggaacgcttg
      721 aaagtcctcc agccgagacg gcagcagcga gaatcctgca gccatcgggt ggccgccgcc
      781 cgtcttcaaa atccccgcct gccgcgctgc gatcaccacc gcgcccaggt catggcccgc
      841 caccgacctt ccggaccctt tcgccatgtc gtccgcaatg ccggccacgc acgccggccg
      901 gttgaagcgc tccttgatgc gccctgccac gatgccgaca acgcccggat gccacgccgg
      961 cccgctgatc atcagcgccg catgcccctt cccggcctgt gcctcggcgg cagccatcgc
     1021 cgcctcgaga atggcggctt caacctcctg ccgctgtcga ttcacggcat cgagcgtcgc
     1081 cgccaatgat gccgcctcaa ccgggtcccc gcacagcaac agccgcagcc cgagatctgc
     1141 ttccgagatt cgccctgccg cattgatcct cgggcccagc gcgaaactgc acgtccccgc
     1201 gttcagccga tcgcgcgact gcgccacatc cagcagtgct gccacgccag gacgtgcccg
     1261 ccgcgccatc accttcagtc cctggcacac cagcgcgcgg ttcactccgg tcaacggcat
     1321 cacgtcgcag attgtcgcaa gcgcaaccag atcgagcaga ccaatcaggt ccggctcctt
     1381 gcgcgcattg aaatagccct gccgacgcag ggttcgcacc attgcgaccg cggccaggaa
     1441 cgccactgcc gccgcgcaca gaaaggacag gccggatgag cagtccagcc ggttcgggtt
     1501 cactgtcgcg acgatggacg gcggcgcccc ttcggccttg tggtgatcca gcaccacgat
     1561 atcggcatcg ccagccacga atgccaacgc ttcggccgcc gccgtgccgc aatcgacgca
     1621 cacgatcagc gatgcgccac gcagcaccag cgaatgcagt gccggaccgt tcggaccata
     1681 gccctccatc atgcggtcgg gaacgtgata gagcacctcg cagccaagct cccgcagcag
     1741 cgtgcacatc aatgcggcac tgcacgcacc atccacgtcg taatcgccga acacgccgac
     1801 cgtttcgcca tgctgcaccg cgtgcgcaag gcgttccgcc gccacatcca tatcgaccag
     1861 cacggacgga tccggcagta acgcccgcaa ggtcggatca aggaaatgag aggcggaatc
     1921 gatgtcgatg ccgcgcgctg ctagcaaccg tccgacgatt tccggcacgc ccatacgttg
     1981 gcatatacct aggccgatac ggtcctggcc gctgcgccag atccaccgtc gcccgctcag
     2041 gctgcggccg acacccagga cgcagtcgct catatgaacg gaaccacagg tgcacacaga
     2101 tagacacaga tgcttccgca caaagcaaat ctgcgtccat ctgtagcctg ccccggactt
     2161 ggtccggggt ccatctgtgg ttccattatt cttatccccg cacccaggtc ttggtgtgga
     2221 agttgtgatg tgcctcaacg aaccgcaccg tgccggactt gctgcgcatc accatggaat
     2281 gtgtcgccgc accatggccg aaccgcttca cgccgcgcaa catcgtgcca gacgtgacac
     2341 ccgtgcaggc gaacatcaca tcacccttgg ccatctcttc cagcgagaac ttccgcttcg
     2401 gatcgcgatg tcccatctgc ttcactcgct cgacctgttc gtcgttctcg aacaacagcc
     2461 gtccctgcat ctgccctc
//
LOCUS       DATGEM010000336         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1501087, whole genome shotgun sequence.
ACCESSION   DATGEM010000336 DATGEM010000000
VERSION     DATGEM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501087"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..473)
                     /locus_tag="VKQ27_10690"
     CDS             complement(33..473)
                     /locus_tag="VKQ27_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY89441.1"
                     /translation="MRRVIGMDIHRTFGEVVIGEDGKLRHAGRVDMTRTALEGFGKSL
                     RATDEVVIEATGNCMAASRVLSPFVARVVIANPLQVKAIAHAHVKTDKVDAGTLASLY
                     AAGYLPEIWTPDAVTERKRRLVARRYQIVRHRTRIKNEVHAILQ"
     gene            621..932
                     /locus_tag="VKQ27_10695"
                     /pseudo
     CDS             621..932
                     /locus_tag="VKQ27_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1079..1324
                     /locus_tag="VKQ27_10700"
     CDS             1079..1324
                     /locus_tag="VKQ27_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLY89442.1"
                     /translation="METVNIHEAKTHLSRLIERASRGESFIIAKAGKPMVKVTPIGAP
                     TGSQVRRLGFMAGQIEVPDDFDRMGEGEIDALFGGDA"
     gene            1321..1707
                     /locus_tag="VKQ27_10705"
     CDS             1321..1707
                     /locus_tag="VKQ27_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY89443.1"
                     /translation="MKLLIDTHLLLWAASAPDKLPAAARDLIDDSENSLLFSVASIWE
                     VAIKRGLGRDGFKVDPRLLRRGLLDNGYEELAITSEHAVSVDSLPPIHRDPFDRLLIA
                     QATVEGIILLTADEHVGDYPGPIRKV"
     gene            1670..1984
                     /locus_tag="VKQ27_10710"
     CDS             1670..1984
                     /locus_tag="VKQ27_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLY89444.1"
                     /translation="MLAIIPALFARYDLTEPFDDPRSLVCPLLLGPAERVVDRDQLRI
                     CREQATRDGWQVVAVYKDAAISGSSVTLRPGIQALLQDAQARKFERGAGGGVRPRVAR
                     SG"
     gene            1941..2099
                     /locus_tag="VKQ27_10715"
                     /pseudo
     CDS             1941..2099
                     /locus_tag="VKQ27_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2339..2527
                     /locus_tag="VKQ27_10720"
                     /pseudo
     CDS             2339..2527
                     /locus_tag="VKQ27_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2766..>3116
                     /locus_tag="VKQ27_10725"
     CDS             2766..>3116
                     /locus_tag="VKQ27_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89445.1"
                     /translation="MAAAPSASDGAGLEKVLKAISGLVAMAEQGLATRAVADKLLALE
                     AQEDVLRVRLAAGPIDMPDIHPNISEIYRRRVERLAEALHQPEERDEAADAIRALIER
                     VTLTPAARRGQGDAT"
BASE COUNT          584 a         1035 c          969 g          528 t
ORIGIN      
        1 cggcaacccc tttctgccaa ccaggtgagc agttactgca ggatagcgtg cacttcgttc
       61 ttgatccgtg tccggtgccg gacgatctga tagcgacgcg caacgagacg gcgtttgcgt
      121 tccgttaccg catctggcgt ccagatctcc ggcaggtagc cggcagcgta caggctcgcc
      181 agggtgcccg catcgacctt gtcggtcttc acatgcgcat gcgcgatcgc cttcacctgg
      241 agcggattgg cgatcaccac ccgcgccacg aagggcgaga gcacgcgcga cgccgccatg
      301 cagttgcccg tcgcctcgat cacgacctca tcggtcgcac gcaagctctt gccgaagcct
      361 tcgagcgccg tgcgggtcat atcgacccgg cctgcatggc gcagttttcc atcttcccca
      421 attaccacct cgccaaaggt tcggtggata tccattccga tcacgcgccg cattggttcc
      481 tcctgtcagc atgacgacgg gagcggtggg cgacacgaca cctacggatc cgcgctctcg
      541 gcgcaaccgg gcgagtcgca gaggcggcca gctactaact cgagctctca gctcatcgta
      601 tacatcggcc tgcccacact ttcgcgctcc cggtgcctct gtcccggatg gtcgcaccat
      661 ataccaaaac caggatgatc tcggcgggga tttcggcacc ggcaaccctc ataccggtta
      721 caaatgcgat cgaggctttg aactcgaagc tcagacgcgc cgtccgagcc aggggccact
      781 tccccagcga cgatgccgcc accaagctgc tctatctgat cttgaaccgg tcggagaaag
      841 agtggaaaat gccgccacgc gagtggtcca tggccaaggc tcaattcgcc gtcatcttcg
      901 gcgagcgctt catcagggcc atggcggcgt aatattcaac cgcccgcccg cacacggaaa
      961 ttctgacagt cccggcgcgc tgccaatggc aacacccctt cggcgatcca acctgatccg
     1021 aattgagaag cggagcgaat ggtgatagac taacgacgct tagtccattg gaggcgtgat
     1081 ggagacggtc aacatccacg aagccaagac gcatctctcg cggctcatcg agcgcgcctc
     1141 gcggggtgaa tccttcatca tcgccaaggc cggcaagccg atggtgaagg tcaccccgat
     1201 cggcgcacca accggtagcc aggtccgtcg cctcggcttc atggccggac agatcgaggt
     1261 gcccgacgac ttcgatcgca tgggcgaggg cgagattgac gcactgttcg gcggcgacgc
     1321 gtgaagctgc tgatcgatac gcaccttctg ctctgggcgg catccgcacc tgacaagctc
     1381 ccggcagcgg cgcgcgacct gatcgacgac agcgagaaca gcctgctgtt cagcgtcgcg
     1441 agcatctggg aggtcgccat caagcgcggc ctcggccgcg atggtttcaa ggtcgaccca
     1501 cggctgttgc ggcggggcct gctcgacaat ggctatgagg agttggcgat caccagcgaa
     1561 catgcggtgt cggtcgacag cctgccccct atccatcgcg atcccttcga ccgtctgctc
     1621 attgcccagg ccacggtcga gggtattatc ctgctgaccg ccgacgaaca tgttggcgat
     1681 tatcccggcc ctattcgcaa ggtatgatct gacggagccg ttcgatgacc cgcgtagcct
     1741 tgtatgcccg ttactcctcg gaccagcaga gcgcgtcgtc gatcgagatc agttgcggat
     1801 ttgccgggag caggcgacac gagacggctg gcaggtggtg gccgtctaca aggacgccgc
     1861 catctcgggt tcaagcgtga cgctccgccc cggcatccag gcgctgctcc aggacgcaca
     1921 ggcgcgcaaa ttcgaacgcg gtgctggcgg aggcgttcga ccgcgtgtcg cgcgatcagg
     1981 ctgacgtcgc gacgctctac aagcatctgc aattcgccgg ggtccagatc gtcaccctcg
     2041 ccgaaggcga aatctcggaa ctccatgtcg atctcaaggg aacgatgaat gcgctattcc
     2101 tgaaggacct ggcgaggaag acccatcgcg gcctgcgggg acgcgtcgag aagggtaagg
     2161 ccggcggcgg cctctgctac ggctatgacg tcgtcaaacg cctcgatagc gcaggcgagc
     2221 ccgtccgcgg cgagcggacc atcaacgaga ccgaggccga ggtggtgcgc cgcgtgttcc
     2281 atgacttcgc caacggcgtc agccccgcac catcgcccag cgcctcaatg cggatggcat
     2341 ccccggcccc ggcggccagc tctggaacgg aacgacgctg cgcggtcacg ccaggcgtgg
     2401 caccggcttc atcaacaacg agctctatgt ggggcgcctg gtctggaacc ggcagcgcta
     2461 tgtgaaggat cccagcaccg gaaagcgggt gtcacgcctc aagcccgagt ccgagtggat
     2521 catcaccgag gtgcccgagc tgcgcatcgt cgacgatcag ctctggcaag ccgccaaggc
     2581 gcgccaggag agcatctctc tgcgctacgc aaccgctatc aaagcgacgc gcgccgctcg
     2641 caaccagctc aatgcgacgc atcgccccag gtcgctgccc tccgggctcg ttttctgcgg
     2701 cgtctgtggt ggcccctatt cactgcgcgg ccagggtcgc ttcgcctgct cggcgcatat
     2761 cgacaatggc agctgcgccc agcgcgtccg acggggcggg actggagaag gtgctgaagg
     2821 ccatcagcgg cttggtggcg atggcggagc aaggcctcgc gacacgcgca gtggccgaca
     2881 agctgctcgc actggaggcg caggaagacg tcctccgcgt tcgcctggct gcgggcccta
     2941 tcgacatgcc ggacatccac cccaacattt ccgagatcta ccgccgcagg gtcgagcgtc
     3001 tcgcagaggc actccatcag ccggaggaac gggacgaagc ggcggacgcg atccgtgcgc
     3061 ttatcgagcg ggtgacgctg accccggccg ctcggcgcgg gcagggcgac gcaacg
//
LOCUS       DATGEM010000337        12809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_917941, whole genome shotgun sequence.
ACCESSION   DATGEM010000337 DATGEM010000000
VERSION     DATGEM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917941"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..595
                     /locus_tag="VKQ27_10730"
     CDS             44..595
                     /locus_tag="VKQ27_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89446.1"
                     /translation="MKYALWGAAFVMAAACLGTPALADDGQFGCPAAGTTMTLSVGSP
                     QTFGPSSGFDCPHGGGLQHAGFANQASSEQVKEASEKIWPLKVGNKISADIYASGGRS
                     SHGTYSCEVQGRESVTVHAGKFDAFKVKCIMEFPQARYQDERMYWWSPELKFPVKFDY
                     QLIQGTAFGTVPLKSWELVSVSH"
     gene            1243..2280
                     /locus_tag="VKQ27_10735"
     CDS             1243..2280
                     /locus_tag="VKQ27_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLY89447.1"
                     /translation="MSKPPHFSRAPTIGEVAQAAGVSRATVSRTFSQPDRLSETTVQR
                     VLAAAERLGYVPNQTARALSTGRAGNIALIVPDVANPFFPPLIRGAQARADEAGYAVF
                     LGDADETPAREDILLGKLAAQVEGFVLAAPRLPEDRLRIHAKRRPLVLINRDVANIPR
                     VLIDTAAGVAAAIDHLVALGHRHIVYVSGPSASWSNQQRQLAAGLAAERTGIRLDRIA
                     THRPTYLAGQECTDELLATGATAVVAFDDLVAQGIMAGLASRGTPVPASLSVVGCDDV
                     LAERTYPPLTTIAAHCAEAGQRAVELLLEMLASGAPRTTPVTIAAELVVRATTAPPPR
                     AASRRPRGGHG"
     gene            2365..3129
                     /locus_tag="VKQ27_10740"
     CDS             2365..3129
                     /locus_tag="VKQ27_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HLY89448.1"
                     /translation="MIHIDLQPTKIALGAAAAAAGAKAIRAAIEERGHANIIVATGMS
                     QFELLAALVAEPGIDWSRVTAFHLDEYIGMPETHPASFRRYLKERFVSHLPNLRAFHF
                     VRGDAADVDAEIERLNALIAAHPIDVTFAGIGENGHLAFNDPPADFAATEGYKRVELD
                     EACRRQQFGEGWFPTLDDVPRQAISMTVQQIMRSALVILAVPDARKAEAVRATIEGAV
                     TNLCPASILQRHPNCQLFLDPPSAGELARAPRSAAR"
     gene            3173..4360
                     /locus_tag="VKQ27_10745"
     CDS             3173..4360
                     /locus_tag="VKQ27_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY89449.1"
                     /translation="MGWLTRNFVCRTAGFAAVIAFAADGCAQSSAATPKAPLVINVMD
                     VAGDLEVSQPALEKFRKEHPELVSKFVFTRATAPELAGKLKAQQDAGSVDIDFVLTGN
                     DALAAGMDQNLWLDILPAYQNRFPDLTQLYLPGAYAMQKMARGQAFVLDWYPSGPLLE
                     YAPDRVKDMPDTAEGLLAWCKAHPGRFMYARPSNSGPGRTFVMGLPYLLGDKDPQDPV
                     AGWDKTWNYLKELDSCIEYYPSGTGATMKELAEGSRDVIATTTGWDINPRYLGIVPAD
                     LKVGTFKGFHWVGDANYMAIPKGGSPEKRDVVLELMAFLLKPEQQAYMYDHGYMYPGP
                     AIKNVPVAMAPAESQETIRKFGRPEYSGLIADNPIEPPLDAKPLVAMFQKWDREIGAK
                     KMN"
     gene            complement(4396..4980)
                     /locus_tag="VKQ27_10750"
     CDS             complement(4396..4980)
                     /locus_tag="VKQ27_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89450.1"
                     /translation="MAALIAVALPQPVDAAATGWLGTNWGDSTDGLGRQFGAAATRLD
                     RSIDFGDSYADLVLKDYDIGGYRFIVYFQMDKHTHGLKRIQIERGRHGAVPQVAKAAF
                     DFLVASYGPPTMACAIRAHTIDEQNLTEEVWQREGNEVRALFREASLNTLDPYLARAV
                     GDFVTGSTAEGLSQQLLIRIAPAGTEPEDCRARK"
     gene            complement(5167..5694)
                     /locus_tag="VKQ27_10755"
     CDS             complement(5167..5694)
                     /locus_tag="VKQ27_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89451.1"
                     /translation="MPRVLVSDMVGFIKKLPHDLVASFRSTLAEALEASLLLCIVDAS
                     DPTCEPQLEVSRTVLREIGARDVPSRLVFNKIDRVSEAARAAILAKHPGAILLSAKAP
                     EDVAALRETIIAFFEDAMVEEQLVLPYAKQRLLSDVYEHARVLSEDYDATGRIITVRG
                     LPGAITRLRRTLAAS"
     gene            complement(5639..6352)
                     /locus_tag="VKQ27_10760"
     CDS             complement(5639..6352)
                     /locus_tag="VKQ27_10760"
                     /inference="COORDINATES: protein motif:HMM:NF027678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HLY89452.1"
                     /translation="MLGKGRLEGLAAITGGTGVAGSLVTVRKSKARQRFEAADVTKPI
                     VPRGEPDLEASPKPEFVIVDHDLSPAQARNLERATGAQVLDRTGVIVEIFHRHAHSRE
                     AKLQVEIARLKCVSSRLRESLTGGGRQQGPGAGESDLELDRRRIRDRLAELKEQLKDI
                     ERDDDQRRSARRDHPRVALVGYTNAGKSSLMRALTGSEVLVADKLFATLDTTVRAAAR
                     DGAARARLGHGRLHQEAAA"
     gene            complement(6434..7975)
                     /locus_tag="VKQ27_10765"
     CDS             complement(6434..7975)
                     /locus_tag="VKQ27_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89453.1"
                     /translation="MRTDNMTTNRVRLLTGCCVFALGLLAGTAQAKDHDHDDRDHFKI
                     EHDSLVISSSTYDPTRGAVAGLRPGSVLPNTAKATTTAISGNNYVTVWSNESVDASFG
                     VTSPILLTGLDASSGRVLHTIGVPEEAVVTSFSSKSELALHLTQDQDERRLVFVGYAG
                     AGVGAIDASNSDAVPGQDPTNPVTFAFGLKYAFPRTIVSMDKHGNFAYTPTINYGGNN
                     GRAALLGSNGLYYSVGNANNGNATTFGASNGTNPDVTETTGLEAVIPLDAPTLYVAIP
                     PSSSAEVDPLLQMVSNGKLDKPGKDDNFRGVTEHRGALYFTKGSGSNGIDTVYTVSSL
                     PTIAGAAEAQISVVPGFPTDSARVTGGNFTPFAVFFANDTTMYVTDEGSGNATDVANH
                     GGLQKWSLVNGVWQLDYVLTQGLIGVVDTNLNGPAGPYPAVTTVGLRNLTGVVGRDGM
                     VTLWATTATSSASSDNGADPNKVVRITDYLPAKSLTGSVTHATFRTIAGPTYGTVYRG
                     VAYAD"
     gene            complement(8857..9687)
                     /locus_tag="VKQ27_10770"
     CDS             complement(8857..9687)
                     /locus_tag="VKQ27_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLY89454.1"
                     /translation="MTTLFDRRRFLVGGAVLLASPAVITAPVFAVTPDEIKRRGKVII
                     GIQGDNPPWGYVDSSGKQQGLDSDMSELFAKELGVPLEYVQLTIANRIPALTSGRVDL
                     LFATMAMLPERAKAVQFSKPYAANIITMVASKGVEIKTAADLSKYVVGVARGSVQDTE
                     VTKNAPAGTTIRRFDGDAPTIQALLSGQVEAVGGNIFYVPRLNEAKPDTYENKLEFTK
                     LYNGACTRLGEREINASLNSFIDKIRANGELARIYPKWMSNPLPAFPDSLPDIPFVAS
                     "
     gene            complement(9735..10376)
                     /locus_tag="VKQ27_10775"
     CDS             complement(9735..10376)
                     /locus_tag="VKQ27_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLY89455.1"
                     /translation="MNASELLFLLDGLKWTIALSAIGFVFGAIGGLGVALARTSGLHP
                     LERCMAGYIALFQGTPLLMQLFMVYYGLALIGLKLDAWQAVAIGFTLHASAFLGEIWR
                     GSIQAIPRGQTEAAKALSLGYVARMRLVILPQALRISLPATIGFLVQLTKGTSLAAIV
                     GFTELARAGTMVSNQIFKPLLVFGVVGALYFTICWPLSLYGTWLERRLAVAAR"
     gene            complement(10387..11055)
                     /locus_tag="VKQ27_10780"
     CDS             complement(10387..11055)
                     /locus_tag="VKQ27_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLY89456.1"
                     /translation="MNYKLDFGAVIDGLPDLLWGCLGTLGLALSGMLLALVIGIGGVV
                     LRGSSYRGLRWLVVSFVELIRNTPFLVQIYFIFFALPLLGLRLDPTPTAIIALGVNGG
                     AYAIEIIRGGVKSISAGQVEAGLALGLRKALVFRLVVLRPALRAIFPSLTSQFILLTL
                     TTSIASAISAYELTSVAQNIENNSFRSFEVYGAITILYFAMSWMLMRMFALIEARYFS
                     YPVK"
     gene            complement(11161..12264)
                     /locus_tag="VKQ27_10785"
     CDS             complement(11161..12264)
                     /locus_tag="VKQ27_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY89457.1"
                     /translation="MSNIAIGVIGTGLIGRKHLQKIAEHPDFDLAGIADVNVDAAAAL
                     NPQAPVFSDHRRLLDEVRPDAVIIASPNQHHAQIGIDCARCGIDILVEKPVTDTLETA
                     AQLIAEVRKAGIRTLVGHHRRHHRQVKTLRALLRDQRIGSLVGVSAIWATYKPDTYFA
                     AGPWRTQPGGGPILINLIHEIDFLRFTVGEIVAVGAITSDRQRGFAVEDTMSALIEFD
                     TGALGSFFASDSAVSPWTTEQGVGESAEFPFSGESSYRFMGTRGALEFPNIVQWSQAD
                     GAQDWNRPIQAQRIHAPTIDPYIAQLDHFRDVVRGVAASVQSVEDGTKTLIATLAVTE
                     AAATGARIDLRGRYEALLAQASAPLDHQGREAL"
     gene            complement(12261..>12809)
                     /locus_tag="VKQ27_10790"
     CDS             complement(12261..>12809)
                     /locus_tag="VKQ27_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLY89458.1"
                     /translation="DGIRLAARFADLCDLARPFGLRVDLEFMRWREVGTLAQAETVLR
                     RAGKSNGAILLDALHLHRSGGKPADLAPLSASALRAAQLCDAGANIPQTEAEVIAEAR
                     EGRLPPGEGVLPLAELLEALPGDVNLSVEMPFPGLEVRSRLARAFDATRRVLAIRSQG
                     TADRARESATFPTRSPSGPRET"
BASE COUNT         2327 a         4099 c         4178 g         2205 t
ORIGIN      
        1 ctgataccat ggctacccta caattgccgt ggcggatcag acgatgaagt acgctctatg
       61 gggcgctgct ttcgtgatgg cggcagcttg cctcgggacg cctgcgcttg ccgatgacgg
      121 ccagttcggc tgtccggcag cgggaacgac catgaccctg tcggtgggat cgccccagac
      181 cttcggaccg tcgagcggat ttgactgtcc ccatggcggt ggcctgcaac atgccggctt
      241 tgccaatcag gcgagttccg agcaggtaaa ggaagcctcg gagaagatct ggccgctcaa
      301 ggtcggaaac aagatcagcg ccgacatcta cgcctccggc ggcaggtcca gccacggcac
      361 ctattcctgc gaggtccagg ggcgcgaatc cgtcaccgtg catgccggaa aattcgatgc
      421 gttcaaggtg aagtgcatca tggaattccc gcaggcacgg taccaggacg agcggatgta
      481 ctggtggtcg ccggagctga aatttcccgt gaaattcgac tatcagctga tccaaggtac
      541 cgcatttggc acggttccct tgaaaagctg ggagttggtc tcggtcagcc attgatctgc
      601 gtgcaggccg ccgacatgga tctgatcgag ggcgagcgcg acaccacgcc cttgattcag
      661 tccatgtcgg agcccgggct caagcgccgc cgcccgtgaa cagaacggcc gtgatcagca
      721 ttgccgccgt ggccgcgacg aatttcacga aataggcgta gaagccataa gtcagatgag
      781 caggcatgac atcctccgac ggttgatccg ggcgaaggcg gcggggctgc gtccggggcg
      841 ttcatttccc cgtgggaccg ctctgcgctt caaccggtaa tcaagaccgt aggatgctgg
      901 cccggccgcg tcttcttcac ttcgacggag ggcggccggc cttccacaga cggcggctgc
      961 gtcgacaaat gaccggccgg catcgacgaa aatccgcgac gggctgcgat ttactccgga
     1021 acgccggcaa atcctggacc ggcggcaccc tattgccgaa cgcgcccccg gccccaaggc
     1081 gcccctggcg gccgctcagc cgaacttgat cgtttcttgc tagcgacacg gacgcgagtc
     1141 cttcgcacga tcacggaccg cattgactct tcatgcaacc ggttgcataa ctgctgcaac
     1201 cggttgcact ggaagaattg aacataatag agcaggcatt tcatgtcgaa gccgccgcat
     1261 ttcagccgcg cccccacgat cggcgaggtt gcacaggccg caggagtgtc gcgagcaacg
     1321 gtttcgcgca cgttcagcca gccggatcgc ttgagcgaga caacggtgca gcgcgtgctc
     1381 gcggcggccg agcgcctggg ttacgtaccc aaccagacgg cgcgggcgct cagcaccggg
     1441 cgtgcaggga atatcgccct gatcgtgccg gacgtggcca accccttctt tccgcccctc
     1501 atccggggcg cccaggcaag agcggatgaa gccggctatg ccgtctttct gggtgacgcc
     1561 gacgaaacgc ccgcacggga ggatatcctg ctcggcaagc tcgcggcaca ggtcgagggc
     1621 ttcgttctgg cagcgcctcg cctgccggag gaccgcctcc gcatccacgc gaaacgccgt
     1681 ccgctcgtgc tcataaatcg cgacgtggcc aatatcccgc gcgtgctcat cgacaccgcc
     1741 gcgggcgtcg ccgctgcgat cgatcatctc gtagccctcg gccatcggca catcgtctat
     1801 gtcagcggac cctccgcctc ctggtcgaac cagcagcggc agctggccgc cggcctggcg
     1861 gcagaacgta ccggtatccg cctggaccgg atcgccacgc accgtccgac ctacctcgcc
     1921 ggccaggagt gcaccgacga actgctcgcg accggtgcga cggcggtcgt cgccttcgat
     1981 gatctcgtcg cgcaggggat catggccggc ctcgcgagcc gggggacgcc cgtgcccgcg
     2041 agcttgagcg tcgtcggctg cgacgacgtc ctggcggagc ggacctatcc gccactgacg
     2101 acgattgccg cgcattgcgc ggaagcgggg caacgcgccg tcgaactgct gcttgagatg
     2161 cttgcctccg gggcaccgcg gaccaccccg gtcacgatcg ccgcggaact cgtcgtccgc
     2221 gccacgacgg cacccccgcc acgcgctgcc tcccggcggc cgagaggcgg acacggatga
     2281 gaactttcac gacgccatga gggggcttaa gcgcccgctg cttcgggttc ccccgcaccg
     2341 ccagaaccag acgatgggat gaccatgatc cacatagacc tgcagccaac caagattgcg
     2401 ctcggcgcgg ccgcggccgc ggccggcgcc aaggcgatcc gcgccgccat cgaggaacgg
     2461 gggcacgcca acatcatcgt tgccaccggc atgagccagt tcgagctcct cgccgcgctg
     2521 gtcgccgaac cagggatcga ctggtcgcgc gtgacggcct tccacctcga cgaatatatc
     2581 ggcatgccgg agacccatcc ggcgagcttc cgccggtatc tcaaggaacg gtttgtctcc
     2641 cacctgccca acttgcgcgc cttccacttc gtcaggggcg acgccgccga cgtcgatgcg
     2701 gagatcgagc gcctcaacgc tctgatcgca gcacacccga tcgacgtgac cttcgccggc
     2761 atcggcgaga acggccacct cgccttcaac gatccgccgg ccgactttgc cgcgacggag
     2821 ggttacaagc gcgtcgaact cgacgaggct tgccgacggc agcaattcgg cgagggctgg
     2881 ttcccgacgt tggatgacgt tccgcgccag gcaatctcca tgaccgtgca gcagatcatg
     2941 cgcagcgcgc tcgtcatcct ggcggtgccc gatgcccgca aggcggaggc cgtacgcgcg
     3001 acgatcgagg gcgcggtgac gaacctctgc ccggcctcga tcctgcagcg ccatccgaac
     3061 tgccagctgt tcctcgatcc gccatcggcc ggcgagctcg ccagggcgcc gcgctccgcg
     3121 gcccgctgag gcccggcagg acccgttctt cacgaagaca aaggagagaa caatggggtg
     3181 gcttactcgg aacttcgtct gccgcaccgc cggcttcgcc gcggtgatcg ctttcgcggc
     3241 cgatggctgc gcacaatcat cggccgcaac gcccaaggcg ccgctcgtca tcaacgtgat
     3301 ggatgtcgcg ggcgacctcg aggtcagcca gccggctctc gagaaattcc gcaaggagca
     3361 ccccgaactc gtctcgaagt tcgtcttcac ccgagcgacc gcacccgagc ttgcaggcaa
     3421 gctcaaggcc caacaggacg ccggcagcgt cgacatcgac ttcgtcctga cgggcaacga
     3481 tgcgctggcc gcaggcatgg accagaatct ctggctcgac atccttccgg cttatcagaa
     3541 ccgctttccc gacctcaccc aactctatct gccgggtgcc tacgccatgc agaaaatggc
     3601 gcgtggccag gccttcgtgc tcgattggta cccgtcagga ccgctgctcg aatatgcccc
     3661 cgaccgcgtg aaggacatgc cggatacggc agaggggctg ctcgcctggt gcaaggcgca
     3721 tccgggccgc ttcatgtatg cgcggccgtc gaactccggt cctggtcgaa ccttcgtgat
     3781 ggggttgccc tatcttctcg gcgacaagga tccgcaggac ccggtcgccg gttgggataa
     3841 gacttggaat tacctgaaag agctcgacag ctgcattgaa tattatccct ccgggacggg
     3901 cgcaacgatg aaggaactgg ccgagggttc gcgcgacgtc atcgcgacca cgaccggatg
     3961 ggacatcaac ccgcgctatc tcggcatcgt gccggcggac ctcaaggtcg gcacgttcaa
     4021 gggcttccat tgggtcggcg acgccaacta catggccatt cccaaaggcg gatcgcccga
     4081 gaagcgggac gtcgtcctcg agctgatggc gttcctgctg aagcccgagc agcaggccta
     4141 catgtacgat cacggctaca tgtatccggg accggcgatc aagaacgtgc cggttgccat
     4201 ggccccggcc gaaagccagg agaccatccg gaagttcggc cggccggaat attcaggcct
     4261 gatcgccgac aatcccatcg aaccgccgct cgacgccaag ccgctggtcg ccatgttcca
     4321 aaaatgggat cgcgagatcg gcgccaagaa gatgaattga cgccggaacc tacatccgat
     4381 tcccggccaa caatctcatt tcctggcgcg gcaatcctcg ggttccgttc cggctggggc
     4441 aatgcggatc agcaattgct gcgacaggcc ttcagcggtc gagccggtga cgaagtcgcc
     4501 gactgcccgc gccagatatg ggtcgagggt attgaggctg gcttcgcgaa agagcgcgcg
     4561 cacctcattg ccctcgcgct gccagacctc ttcggtcagg ttctgctcgt cgattgtgtg
     4621 ggcgcggatg gcgcaggcca tggtcggcgg cccatagctt gccaccagga aatcgaaggc
     4681 ggccttggcg acctggggca cggcaccgtg tcggccgcgt tcgatctgga tgcgtttcag
     4741 gccgtgcgtg tgcttatcca tctggaagta aacgatgaag cgatagccgc cgatgtcgta
     4801 atccttgagc acgaggtcgg cataggaatc gccgaagtcg atcgagcgat cgaggcgcgt
     4861 cgccgcggcg ccgaattgcc gacccagccc atcggtgctg tcgccccagt tggtgccgag
     4921 ccacccggtc gcggcggcat cgacgggctg cggcaaagcg acggcaatca gcgccgccaa
     4981 caatataggg tgcctaggca tggttggaac ggcacatggc gatcagctcc ggtcgggtga
     5041 accataccag atcgccgtgc cggcaagcga cgcggccgga cgccgcttta gatcagtccc
     5101 cggccacgcc cggaattaaa aaagacggag cacggacgcc tcaccgtttc ccatcggcgc
     5161 tcgaattcac gaggcggcga gggttcttcg caggcgcgtg atcgcgccgg gcaggccgcg
     5221 caccgtgatg atccgaccgg tggcgtcata gtcctcggag agcacgcgcg cgtgctcata
     5281 cacgtcgctg agcagccgct gcttcgcata gggcagcacc agttgctcct ccaccatcgc
     5341 atcctcgaaa aacgcgatga tcgtctcgcg cagtgccgcc acgtcctcgg gggccttggc
     5401 ggagagaagg atcgcgccgg ggtgcttggc gaggatggcg gcgcgcgccg cctcgctcac
     5461 gcgatcgatc ttgttgaaga ccaggcgcga ggggacgtcc ctggcaccga tctcgcgcag
     5521 cacggtgcgg ctcacctcga gttgtggctc gcacgtcgga tcggacgcgt cgacgatgca
     5581 cagcagcagc gacgcctcaa gcgcttcggc gagcgtggat cggaacgagg cgacaagatc
     5641 atgcggcagc ttcttgatga agccgaccat gtccgagacg agcacgcgcg gcaccgtctc
     5701 gggctgcagc gcgcaccgtc gtgtcgagcg tcgcgaacag cttgtccgcg accaggacct
     5761 cgctgcccgt gagcgcgcgc atgagcgacg acttgcctgc gttcgtgtag ccgaccagtg
     5821 cgacccgggg gtgatcccga cgcgcggacc gacgctggtc gtcgtctcgc tcgatatcct
     5881 tcagctgctc tttcagctcg gcgaggcgat cgcggatcct gcggcgatcg agctcgagat
     5941 cggactcgcc cgcgccagga ccctgctgcc gcccaccgcc cgtcagcgat tcccgcaggc
     6001 gcgacgagac acacttcaac cgcgcgatct caacctgcag cttcgcctcg cggctgtgcg
     6061 catggcgatg gaagatctcg acgatcacgc cggtccgatc gaggacttgc gcgccggttg
     6121 cacgctcaag gttgcgggcc tgggccggcg agagatcatg atcgacgatc acgaactccg
     6181 gctttggcga cgcctccaga tcgggctcgc cccgcgggac aatcggcttc gtcacgtcgg
     6241 cagcttcaaa gcgctggcgt gccttcgact tccggacggt caccagcgag ccggcgacgc
     6301 ccgtcccgcc ggtaatcgcc gcgagcccct cgagccgccc cttgccgagc actgtcgcac
     6361 cgtcgatctc ctcacgctag cgggcgcaga tcgaccagcc cgaccgatcg gccgggctgg
     6421 tcggtcgcct gcgtcagtcg gcgtaagcga cgccgcgata gaccgtgccg taggtcggcc
     6481 cggcgatcgt ccggaacgtc gcgtgcgtca ccgagccggt caggctcttg gccggcagat
     6541 aatctgtgat ccgcaccacc ttgttcggat cggcgccatt gtcgcttgag gcgctcgagg
     6601 tcgctgtcgt ggcccagagc gtcaccatgc cgtcccgacc gacgacgccg gtcaggttgc
     6661 gcagaccgac cgtggtcacg gccggatagg ggccggcggg cccgttcaga ttggtatcga
     6721 cgacaccgat caggccctgg gtcagtacat agtcaagctg ccagacgcca ttgaccaggc
     6781 tccatttctg aaggccgcca tgattagcca cgtcggtcgc gttgccggag ccttcatccg
     6841 tgacgtacat cgtggtgtcg ttcgcgaaga acacggcaaa cggcgtgaag ttgccgccgg
     6901 tcaccctggc cgaatccgtc ggaaagcccg gcacgaccga gatctgagcc tcggccgcgc
     6961 ccgcgatcgt cggaagcgag ctgacggtat agaccgtgtc gatgccattg ctgccgctgc
     7021 ccttggtgaa gtagagcgca ccgcgatgtt cggtcacgcc gcggaaattg tcgtccttgc
     7081 cgggtttgtc gagcttgccg ttggacacca tctgcaacag cggatcgact tcggccgacg
     7141 aggacggcgg gatcgccaca tagagcgtcg gcgcgtcgag cggaataacg gcttcgaggc
     7201 ccgtcgtctc ggtgacgtcg ggattcgtgc cgttgctggc gccgaaggtc gtcgcattgc
     7261 cgttattggc attgccgacc gaataataca ggccgtttga accgagcagc gcggcgcgac
     7321 cgttattgcc gccgtaattg atcgtcggcg tgtaggcaaa gttgccgtgc ttgtccatcg
     7381 aaacgattgt gcgggggaac gcgtacttca gcccgaacgc gaaggtgacc gggttcgtcg
     7441 gatcctgccc cgggaccgcg tccgagttcg acgcgtcgat cgcaccgacg ccggccccgg
     7501 cgtaaccgac gaatacgaga cggcgctcgt cctgatcttg ggtaagatgg agggccagct
     7561 cagacttcga agaaaagctg gtgacgactg cctcctccgg cacgccgatc gtgtgcaaga
     7621 cgcgaccgct ggatgcatcg agaccggtca acaggattgg cgaggtgacg ccgaacgagg
     7681 catcgaccga ctcattgctc cagacggtca cataattgtt gccggagatg gccgtggttg
     7741 tcgcctttgc cgtgttcggc aataccgagc cgggccttaa tccagcgacc gcaccccgcg
     7801 tcgggtcgta ggtgctgctg ctgatcacca gcgagtcatg ctcgatcttg aaatggtctc
     7861 tgtcgtcgtg atcgtggtcc ttggcctggg cggtcccggc cagcagcccg agcgcaaaaa
     7921 cacagcatcc ggtgagcagt cgaacgcgat tggtcgtcat attgtcggtg cgcatggtac
     7981 ctcggaatga ctctatgggt ggaatctgtt cgagaaaagt gaatggaaaa tattccgtta
     8041 atatttcaac tctgcgaccg gtgagctgtc gttccgtgac ggcgggagag agatcccggc
     8101 ggattgaagc cgggcacggg cgagattact tgctgtaacg caattcgtcc ctttgagggg
     8161 atggcacgcg ctggagaaat gccagtccgc tgcggttcag cagccagagt ttttgccgct
     8221 tcactgttta tagtggagtt ttcgatcgat accgccgcgc gcccggaaag agtagacgta
     8281 tccaatgagg cacgcattcg ccagggggcg agtgctatca atctggatca tccgtgtccg
     8341 gcgaagaggg gtggagtgtt ggatcagagc attgaagcag ccgagttggg cgctctgcca
     8401 cccagccgct tttcggctcg aacgggccga aaccggaatg gccgcctcgg agaaccaact
     8461 agcgatagcg gacaccgggt gacaaccttg cggaggtcgg agccgaaggg cagaacggcc
     8521 agcccaatgt agagcaagca cggggcgaaa ccagccgagc actagaaagt tcgagcggca
     8581 ttgacccgga cagcttgagg tcggccgtca agcggccgac tggcggactt gcagcgtcgt
     8641 gccctctcag cagagtgtgg accgaccgca tagcgcgatc ggccaccagg tgcctagggc
     8701 gcttcatcgg tcggcctgca accccggcca gatggccgcc cgatgaagcc acagcgttcc
     8761 ggccgaggcc ggtccaagct cgggggcaag ctcaacgttg ggtcggactc taactccatc
     8821 tggaggaaat tacgggggtc aggtcaacat ggacgatcag ctcgccacga acgggatatc
     8881 gggaaggcta tcggggaatg ccggaagggg attgctcatc catttcggat agatcctcgc
     8941 cagttcaccg ttcgcccgga tcttgtcgat gaagctgttg agggaggcgt tgatctccct
     9001 ctcgcctagg cgtgtgcagg cgccgttgta gagcttcgtg aattcgagct tgttctcata
     9061 ggtatccggc tttgcttcgt tgaggcgcgg cacatagaag atattgccgc ccaccgcttc
     9121 gacctgaccg gacagcaagg cctggatcgt cggcgcatcg ccgtcgaagc gacggatggt
     9181 cgtgccggca ggcgcgttct tcgtcacctc ggtgtcctgc acgctgccgc gcgcgacgcc
     9241 gaccacgtat ttgctgaggt ccgcggccgt tttgatctcg acacccttcg acgccaccat
     9301 ggtgatgatg tttgccgcat agggcttgct gaactgcacg gctttggcgc gctcgggcag
     9361 catcgccatg gtggcgaaca gcaggtcgac gcgccctgag gtcagcgcgg ggatgcggtt
     9421 ggcgatggtc agctggacat attcgagcgg aacgcccagc tccttggcga agagctcgga
     9481 catgtctgaa tccagtccct gctgcttgcc gctggagtcg acgtaacccc agggcggatt
     9541 gtcgccttgg atgccgatga ttactttgcc gcggcgcttg atctcgtccg gcgtgacggc
     9601 gaataccggc gcagtgataa cggcgggtga agccaacagc acggcgccac ccaccagaaa
     9661 gcggcgtcga tcgaacagcg tcgtcatgga gcgcgtcatt cttgtcctcc cctattgagg
     9721 taccgccgtt caggtcatcg cgccgccacg gcgagccggc gctccagcca tgtcccgtag
     9781 agggacagcg gccagcagat ggtgaaatag agggcgccca cgacgccgaa gacgagcagg
     9841 ggcttgaaga tctggttgga gaccatggtt ccggcgcggg cgagctcggt gaagccgacg
     9901 atcgcggcga gcgaggttcc cttggtgagc tgcacgagga acccgatcgt cgccggcagc
     9961 gagatgcgaa gggcctgcgg caggatgacg agccgcatcc gtgcaacgta gccgaggctg
    10021 agcgccttcg cggcctcggt ctgtcccctc ggtatcgcct ggatcgagcc gcgccagatc
    10081 tcgccgagga aagcgctcgc atgcagcgtg aagccgatgg cgaccgcttg ccaggcatcg
    10141 agcttcaggc cgatcagggc cagcccgtag tagaccatga agagctgcat caggagcggc
    10201 gtgccctgaa acagggcgat gtaaccggcc atgcagcgtt cgagcggatg cagtccggat
    10261 gtgcgggcga gcgccacgcc gagccccccg atcgcaccga agacaaagcc gatcgccgaa
    10321 agggcgatgg tccatttgag gccatcgagc aggaaaagga gttcgcttgc gttcatggcg
    10381 cgcccctcat ttcacggggt agctgaaata gcgggcttcg ataagggcaa acatccgcat
    10441 caacatccac gacatcgcga agtagaggat ggtgatcgct ccgtagacct cgaagctgcg
    10501 gaaggaattg ttctcgatgt tctgtgcgac cgaggtcagc tcataggcgg aaatggcgga
    10561 ggcgatgctc gtcgtcagcg tgagaaggat gaattggctg gtcagcgagg gaaagatcgc
    10621 gcgcagggcc ggccgcagga cgacgagccg aaacaccaga gccttgcgaa ggcccagggc
    10681 caggccggcc tcgacctgcc ccgcgctgat cgatttcacc ccgcctctga tgatctcgat
    10741 cgcataggcg ccgccgttca cgcccagggc aatgatcgcg gtcggcgtcg gatcgagccg
    10801 gagccccagg agcggcagcg cgaagaagat gaagtagatt tgcaccagga acggcgtgtt
    10861 gcggatcagc tcgacgaagg agacgacgag ccagcgcagg ccgcggtagc tggagccgcg
    10921 cagcacgacc ccgccgatgc cgatcacgag cgccagcagc atgccgctca gcgcgaggcc
    10981 cagcgtgccg aggcagcccc acaagaggtc gggcaggccg tcgatgacgg cgccgaaatc
    11041 aagcttgtag ttcaaggcgc ttcccctctg cttcggcgga accttcttgc gggtccgatc
    11101 ttgggtgtcg tgcggacttc tatcctcgtg ttgcggcttc tccccattgc ccccgcaggg
    11161 tcacaaggct tcgcgacctt gatgatcgag gggggccgaa gcctgcgcca aaagcgcctc
    11221 gtagcggccg cgcagatcga tccgcgcccc tgtcgccgcc gcttcggtga cggcgagcgt
    11281 cgcgatcagc gtcttcgtgc cgtcctcgac gctctgcacc gaggctgcca cgccccggac
    11341 cacatcgcgg aaatgatcca gctgggcgat ataagggtcg atcgtggggg cgtgaatgcg
    11401 ttgggcctgg atcggccgat tccaatcctg cgcgccatcg gcttgcgacc attggacgat
    11461 attggggaat tccagcgcgc cgcgggttcc catgaaccgg tagctgcttt cgccgctgaa
    11521 cgggaattcc gccgattcgc ccacgccttg ctcggtggtc cacggcgaga cggcgctgtc
    11581 gctggcaaag aaggaaccga gcgccccggt gtcgaactcg atcagcgcgc tcatcgtgtc
    11641 ctcgacggcg aagccgcgct ggcgatcgga ggtgatggcg ccgacggcga cgatctctcc
    11701 gacggtgaag cgcaggaaat cgatctcatg aatgaggttg atcaggatcg gtccgccgcc
    11761 cggctgcgtc cgccatggcc cggccgcgaa ataggtgtct ggcttgtagg tggcccagat
    11821 cgccgagacg cccaccaggc tgccgatccg ctgatcccgc agcagagccc gcaacgtctt
    11881 gacctgccgg tgatgacggc ggtggtggcc gaccagcgtc ctgatgccgg ccttgcggac
    11941 ttccgcgatg agctgggccg ccgtctcgag cgtgtcggtg acgggctttt cgacgaggat
    12001 gtcgatgccg caccgcgcgc agtcgatgcc gatctgggca tgatgctgat tgggtgaggc
    12061 gatgatcacc gcatcgggcc ggacctcatc gagcagacgg cgatgatcgg agaagacggg
    12121 ggcttgcgga ttgagcgcgg cggccgcgtc gacattgacg tcggcgatcc ctgccaggtc
    12181 gaagtccgga tgctcggcga tcttctggag atgcttgcgt ccgatcaggc ccgtgccgat
    12241 gacgccgatt gcgatattgc tcatgtttct ctcggtcccg atgggctgcg tgtggggaac
    12301 gtggcgcttt cccgggcgcg gtctgccgtt ccctgcgatc ggatcgccag cacgcgccgc
    12361 gtcgcatcga aggccctcgc cagacgcgat cggacctcga ggccggggaa aggcatctcg
    12421 acgctgaggt tgacgtcgcc cggcagagcc tcgagcagct cggcgagcgg caggacgcct
    12481 tcgcccggcg gcagtcgccc ttccctggcc tcggcgatca cttcggcctc ggtctgcggg
    12541 atattcgccc cggcgtcgca caattgagcc gcgcgcaagg cgcttgccga gagaggtgcg
    12601 aggtcggccg gctttccgcc ggaccgatgg agatggagcg cgtcgagcag gatggcgccg
    12661 ttggatttgc ccgcccggcg caagacggtt tcggcctgcg ccagggtgcc gacctcgcgc
    12721 cagcgcatga attccagatc gacgcgaagc ccgaacggtc gggcgagatc gcacagatcg
    12781 gcgaaacggg cggccagccg gatgccatc
//
LOCUS       DATGEM010000338         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_346840, whole genome shotgun sequence.
ACCESSION   DATGEM010000338 DATGEM010000000
VERSION     DATGEM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346840"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VKQ27_10795"
     CDS             complement(<1..352)
                     /locus_tag="VKQ27_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89459.1"
                     /translation="MARFNAVDLVLWILRIGVLAVVSVGTIATLITNRYTAAQWFDFV
                     MFGLTIGSIYAQIALGYTMVYGVLRLINFAHGDIVMTGAFAGYFVATPLDRSGFLEAH
                     PVLGMGAILAVAIIT"
     gene            complement(395..1654)
                     /locus_tag="VKQ27_10800"
     CDS             complement(395..1654)
                     /locus_tag="VKQ27_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY89460.1"
                     /translation="MRITFNKCGPALAKVLAAVGLLAIAHAASAATVGPVTDPLGVIR
                     IAKGAPIQIGAYWVLSGADTALGLDEKRGVEIAFKDLGGKVQGHPLKLNAEDDGCNAE
                     GGQTAATKLAANPQTVIVLGPACSSAATPGAPILWQQGVVDICTACTAPALTAADRKP
                     EYEGFARTVFSDSEQGKADATYVHNVVKAGSVVTIHDGSPYAQQLQQVFADNFKKLGG
                     KVLSQEAIAPTDVDMHPVLTRIASEKPDLIYFPVFTAAAAQILRQAKETPGLEKTKLM
                     GGGSLLSADFIEAAGPSVVGFIIGYPDLSPEAMGKGYPKFVEEYKKAYNEAPISGYHA
                     NAYDGAMLAIKAIEKVAKSDKDGNLYIGKKALRDAVFAEKFEGISGPIACDKYGECSQ
                     FKPSILQFSSSDPKTFGIGQNPKKIWP"
     gene            complement(1744..2886)
                     /locus_tag="VKQ27_10805"
     CDS             complement(1744..2886)
                     /locus_tag="VKQ27_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLY89461.1"
                     /translation="MDTDNWIGTWTAAPAPAESGAFANQTLRMNPRISLGGNQVRVRL
                     SNAYGTRPLRIGAAHIGLRANGPAIVEGSDRALTFGGDRAAVIAAGAVLVSDPVELQV
                     APLADVAVTVFLPGDVPANFGITGRYARQTNYISPPGDFSAETVMPVGRLTDDWFFVC
                     GIDVLAPPDTGAVVALGDSLTDANISTHDAFARWPDQLARRLIARGRGRPMAVLNQGL
                     GGNRILHDIRGDSGLRRFDRDVLAQPGVTHVIVMLGTNDLRNRWAKPEEEVTAEQMVA
                     GLKQMALRAHARGIRIFGGTLTPFENETFMVGAWTPAREAVRLAVNAWIRDSGAFDAV
                     VDFDAGVRDPEHPARMLPIYDCGDHLHPSDAGYNRMGDIIDLSLFE"
     gene            2945..3376
                     /locus_tag="VKQ27_10810"
     CDS             2945..3376
                     /locus_tag="VKQ27_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HLY89462.1"
                     /translation="MRYGFAVLVLLLGACSQSGETRNFSLSRDSAPETVAATQMPLSM
                     PPDLAMRPKRAGALMPNSEASAPQPDVSTGSAGQDAFLDAAGGASPSSDVRGQIDESS
                     GLVYPNGEFVSRVMTWTPPPGYHPLATQASKGWLSGWFNWF"
     gene            complement(3363..4202)
                     /gene="gluQRS"
                     /locus_tag="VKQ27_10815"
     CDS             complement(3363..4202)
                     /gene="gluQRS"
                     /locus_tag="VKQ27_10815"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HLY89463.1"
                     /translation="MIVTRFAPSPTGYLHLGHAFSALFAWRRAREVGGRFLLRLEDID
                     PTRCRPEYAAAILEDLAWLGLDWDGELRIQSQHLADYRAALDQLAARDLLFPCFCTRA
                     DIQRSAAAPHTPDGTPLYPGTCRKLTAAERAARIAAGIPHAQRLDMQRALACVSTRLT
                     FEEDAEGSLSCYPERFGDVVLARKDAPASYHLCVTHDDALQGVTLVTRGADLKRATDL
                     HRLLQSLMHWPAPRYAHHGLLTDDTGRRLAKRDSAATLRGLREAGQTPEQVRALIAAV
                     RTN"
     gene            complement(4199..>4874)
                     /locus_tag="VKQ27_10820"
     CDS             complement(4199..>4874)
                     /locus_tag="VKQ27_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89464.1"
                     /translation="AHATAHLAYEEGDPPAARSFLTNWLTTYPREGALYSHLSWHLAL
                     AHLEAGDIPIANSLFREAFAPDVHTGPPRGKLNDGVSFLWRRELAGQPRDPAAWRAMH
                     AFVMSSFPRPGAAFSDMHIALAQAAAGDDAALSTRTQQIEVLAQQGLYSSGPCVPAVA
                     RAFAAFEQREYPATIAALEPIADDLERIGGSHAQLDLVRFTLLKAYLAADRPDDANRL
                     LRARRR"
BASE COUNT          842 a         1551 c         1637 g          844 t
ORIGIN      
        1 atgtgatgat tgccaccgcc aggatcgcgc ccattcccag caccggatgc gcctcaagga
       61 agcccgaccg atccagcggc gttgccacga aatagcccgc gaacgcaccg gtcatcacga
      121 tatcgccgtg cgcgaagttg atcagccgca gcacaccgta gaccatcgtg tagcccagtg
      181 cgatctgcgc gtagatactg ccgatggtca ggccgaacat gacgaagtcg aaccactgcg
      241 cggctgtata gcggtttgtg atcagcgtcg cgatcgtgcc aacagagacc acggccaaaa
      301 cgccgatgcg caggatccac agaaccagat cgacggcatt gaaacgggcc atggctgtgc
      361 gccatggccc gctggcagtg acagtcccgc tcacctacgg ccagatcttc tttgggttct
      421 gaccaatacc gaacgtcttt ggatccgacg aggagaactg caatatcgaa ggcttgaact
      481 gcgagcactc accatacttg tcacaggcga tcggcccgct gatgccctcg aacttctcgg
      541 cgaagaccgc gtcacgcagc gccttcttgc cgatatagag gttgccgtcc ttgtcgctct
      601 tggcaacctt ttcgatcgcc ttgatcgcca gcattgcacc atcataggca ttggcatgat
      661 agcctgagat cggcgcctcg ttatacgcct tcttgtattc ttcgacgaac ttcgggtagc
      721 ccttgcccat ggcctcgggc gacaggtcgg gatagccgat gatgaagcca acgaccgatg
      781 gccccgcggc ctcgatgaag tcagctgaca gcagcgagcc gccgcccatc agcttggtct
      841 tctccaggcc cggcgtctcc tttgcctggc gcaggatctg cgccgctgcc gccgtaaata
      901 cggggaagta gatcagatcc ggcttctcgc tggcgatgcg cgtcagcacc ggatgcatgt
      961 ccacgtccgt cggcgcgatg gcttcctgcg acagcacctt gccgcccaac ttcttgaagt
     1021 tgtcggcaaa cacctgctgc aactgttgtg catacgggct gccgtcgtgg atcgtcacca
     1081 ccgatcccgc cttgacgacg ttgtgcacat aggtcgcgtc ggccttgccc tgctcgctgt
     1141 cactgaacac ggtgcgcgca aagccctcat attccggctt gcggtctgcg gcagtcagtg
     1201 cgggcgccgt gcatgccgtg cagatatcca cgacaccctg ctgccagagg atcggcgcgc
     1261 ccggtgtcgc tgccgacgag cacgccggtc caagcacgat cacggtctgc gggttggccg
     1321 ccagcttggt tgccgcggtc tggccacctt cggcgttgca gccatcatct tctgcgttca
     1381 gcttcagcgg gtggccctgc accttgccgc cgagatcctt gaaggcgatc tcaacgccac
     1441 gcttctcgtc cagccccagc gccgtatcgg caccggacag cacccaatag gcaccaatct
     1501 gtataggcgc gcctttggca atgcgaatca ccccgagcgg atctgtcacc ggcccgaccg
     1561 tcgccgccga ggcggcatgc gcgatggcga gaagcccgac ggccgcgagt actttcgcca
     1621 gcgccggacc acacttgttg aacgtgatgc gcaaagtccc cctcctgttt atgtgcgacc
     1681 gcgcctccca gccacgcgac cgtgcagaac tttgcggcgg cgcagcagaa caagtcaacg
     1741 ggcttattcg aacaacgaca ggtcaatgat gtcccccatc cgattgtaac cggcatcact
     1801 cgggtgtaga tggtcgccac aatcgtatat cggcagcata cgcgccggat gttccggatc
     1861 gcggacgccg gcgtcgaaat ccaccacggc gtcgaacgcg ccgctatcgc gaatccacgc
     1921 gttcaccgcc aggcgaaccg cctcgcgcgc cggggtccag gcgcccacca tgaatgtctc
     1981 gttctcgaac ggtgtcaggg tgccgccgaa gatcctgatg ccgcgcgcgt gcgcccggag
     2041 cgccatctgc ttcaggccgg cgaccatctg ttcggcggtt acctcctcct cgggcttcgc
     2101 ccagcgattg cgcaggtcgt tggtaccgag catcacgata acatgtgtca cgcccggctg
     2161 tgcgagcacg tcgcggtcga accgtcgcaa gccgctgtca ccacgaatgt catgcaggat
     2221 gcggttgccg ccgagaccct ggttcaacac cgccatcggc cgacctctgc cgcgtgcgat
     2281 cagccggcgc gcgagctgat ctggccagcg cgcgaatgcg tcgtgcgtgg agatgttcgc
     2341 gtcggtgagc gaatcgccaa gtgccacgac ggcgccggta tccggcggcg ccagtacgtc
     2401 gatgccgcag acgaagaacc agtcgtcggt cagccgtccc accggcatca ctgtctcggc
     2461 ggagaaatca ccgggcggcg agatgtagtt ggtctgccgt gcgtagcggc cagtaatgcc
     2521 gaaatttgcc ggcacatcgc ctggcaggaa gactgtcacc gcgacgtccg ccagtggcgc
     2581 tacctgcagt tcaaccggat cgctgaccag gaccgctcca gccgcgatca cggccgcgcg
     2641 gtcgccgccg aatgtcagag cgcgatcgga accttcgacg atcgccggac cgttcgcccg
     2701 aagtccgata tgcgcggcgc cgatacgcag cgggcgcgtg ccataggcgt tggacagccg
     2761 cacgcgtacc tggttgccgc ccaggctgat cctagggttc atccgcaagg tctggttggc
     2821 gaatgcgcca ctctcggcgg gtgcgggcgc tgccgtccag gttccaatcc agttatctgt
     2881 gtccatggcg tgacctcccc agcagcggac agtataagca atgccgccga tgcgggagac
     2941 tttgatgcga tacggctttg cagtcctggt gctgctgctt ggggcgtgca gccagagcgg
     3001 ggagacgcgc aatttcagcc tctcgcgtga ttccgcgccg gaaacggtgg cagcgacgca
     3061 gatgccgctg tcgatgccgc cggacctggc gatgcggcca aagcgtgcgg gcgccctgat
     3121 gcccaactcc gaggccagcg cgccccagcc ggacgtgtcc actggcagcg caggacagga
     3181 tgcattcctg gatgccgctg gcggagcctc cccgtcatcc gatgtgcgtg gccagatcga
     3241 tgagagttcc ggcctggtct atcctaacgg cgagttcgtc agccgcgtca tgacctggac
     3301 gccgccgcct ggctatcacc cgttggcgac gcaggccagc aaggggtggt tgagcggctg
     3361 gttcaattgg ttctgacggc ggcgatcaga gctcgcacct gctccggcgt ctgacccgcc
     3421 tcgcgtagac cgcggagcgt agcagcactg tcccgcttcg ccagccggcg gccggtgtcg
     3481 tcggtcagca gcccgtggtg ggcatagcgc ggtgctggcc agtgcatcag cgattggagc
     3541 aggcggtgca ggtccgtggc ccgcttcaag tcggcgccgc gcgttaccag tgtgacgccc
     3601 tggagcgcgt catcgtgcgt gacgcacaga tggtagctcg cgggtgcatc ctttcgcgcc
     3661 agcaccacat cgccgaatcg ctctgggtag caggagagcg aaccctcggc gtcttcctcg
     3721 aaggtgaggc gggtgctgac gcacgcaagc gctcgttgca tgtccaggcg ctgcgcatgg
     3781 gggataccag cggcgatgcg cgctgcccgt tcggcggcgg tgagtttacg gcaagtacct
     3841 gggtagagcg gggttccatc cggcgtatgg ggtgccgcgg ccgatcgctg aatgtcggcg
     3901 cgggtgcaga agcaggggaa caggagatcg cgcgcggcaa gctgatcgag cgcggcccgg
     3961 tagtctgcga ggtgctgcga ctggatgcgg agctcgccat cccagtccaa accgagccag
     4021 gccaggtcct caaggatcgc cgcagcgtat tcggggcggc agcgcgtggg gtcgatatcc
     4081 tccaggcgca gcaggaaccg gccgcccacc tcgcgggcgc ggcgccaggc gaacagcgcg
     4141 ctgaaggcat ggccgaggtg aaggtagccc gtggggctgg gcgcgaatcg ggtgacgatc
     4201 aacggcggcg ggctcgtagc aggcggttcg catcgtcggg gcggtcggct gcgagatagg
     4261 ccttgagcag ggtgaagcgc acgaggtcaa gctgggcgtg gctcccgccg atgcgttcca
     4321 aatcgtcggc gatcggctcg agggcggcaa tggtagcagg gtattcgcgc tgctcgaagg
     4381 cggcgaaggc gcgggcaact gcggggacgc aggggccgga cgagtagaga ccctgctgcg
     4441 ccagaacttc gatctgctgg gtgcgggtgg aaagtgctgc gtcgtcaccg gccgcggcct
     4501 gggcgagggc gatgtgcata tccgagaagg cggcgcccgg acgggggaaa gagctcatga
     4561 cgaaggcgtg catcgcgcgc caggcggcgg ggtcgcgggg ttggccggca agctcgcggc
     4621 gccagaggaa ggaaacgccg tcgttcagct tgcctcgggg cgggccggtg tggacatcgg
     4681 gggcgaaggc ttcgcggaaa aggctgttgg cgatggggat gtcgccggct tcgaggtggg
     4741 cgagtgccag gtgccaactg aggtggctgt agagggcgcc ctcccgcggg taggtggtga
     4801 gccagttggt gaggaaggaa cgggcggccg gaggatcgcc ctcctcgtag gccagatggg
     4861 cggtggcgtg ggcc
//
LOCUS       DATGEM010000339         5012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1326398, whole genome shotgun sequence.
ACCESSION   DATGEM010000339 DATGEM010000000
VERSION     DATGEM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326398"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..998)
                     /locus_tag="VKQ27_10825"
     CDS             complement(78..998)
                     /locus_tag="VKQ27_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLY89465.1"
                     /translation="MSELRVMATGLCFPEGPVAMADGSVVLVEIERQTVSRVLPDGSV
                     QVIAHTGGGPNGLAIGPDGAFYVCNNGGFAWRLEENLFRPAMVASDYVTGRIQRVDAR
                     TGSVSVLYDRCGAIKLNGPNDIVFDALGGFYFTDLGKTRARDRDNGVIYYALADGSRI
                     VEVAYPALTPNGIGLSPDGTVLYVAETEPARVWAFDITSPGVVAKQGWPSPHGGRLVI
                     GLPGLQRFDSLAVDASGNICVATLITGCISVIAPDGRLMRQVPMGDPVTTNICFGGPD
                     RRTAYITLSGTGQLVAMEWPEPGLRLAHAA"
     gene            1091..2449
                     /locus_tag="VKQ27_10830"
     CDS             1091..2449
                     /locus_tag="VKQ27_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLY89466.1"
                     /translation="MRLPLPTPWSGRFDQGTPLLLMVGAAFAASAAVAVMVPRWFWLP
                     LGIIAVGGIGILAYRHTIAFCVAWLLIVGATLEMALADTIGLGLYQPVIAAVKATEIL
                     LAALCVLRYGPYSDVFNPGLAFLAMFGFGLVHGLHPGLTPVDSLRSLIGSIAPFAFGF
                     SRLSARWARAIVRATIWIPLLSVAGGAALSLAGLRDLFVESGGERLAGLGHPAFLAGF
                     CLAAIYGCLIELYREGLSRWMILLAVNFAILVLTGARAPMAYGVAVTGLTLVFVRSDA
                     FPRRCRILPLLLAACLLPLLLVLAGHLTEVRLFNVLTTEAANLSGRELLWPPFQRSAD
                     ASPWFGWGVGAGNAIIPPDSELARIIQSWAAHNEYLRMSVEGGQIGRGLLIALFVLWV
                     VHHTRVLCRTDRAIMRLVFLAFAAHAYTDNVLISTTACVFFAFVTAVFARGRLMNTAE
                     VA"
     gene            2480..3439
                     /locus_tag="VKQ27_10835"
     CDS             2480..3439
                     /locus_tag="VKQ27_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HLY89467.1"
                     /translation="MLRAFLLLLPMALWANVAFAAGLAYVVNSGAASISLIEMSTEKE
                     LRVIPLLREPHHLALSPDGKSLLVGDTVGNEMFFFDPATAALQKRLPVADPYQLAFSP
                     NGKFLVVNGLARNQVDVYDAASMQLLKRFPIASMPSHLDYAPDSSRVFVSLQGTDSLV
                     AIDLNKLEVLWKVKIGSTPAGVMWHNGKVLVADMGTDYVAVTEPSDGRVVERIHTGKG
                     AHNLFLSPDRKIIWVNNRVGGTTTSVDATTLAVIRTYRIPGGPDDIAFAPDGKLWITR
                     RWAEKVAVLDPQTGDYQTIDVGRSPHGIFLYPKGDAGRSVAAR"
     gene            3563..4513
                     /locus_tag="VKQ27_10840"
     CDS             3563..4513
                     /locus_tag="VKQ27_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HLY89468.1"
                     /translation="MPDPFDLTAGWLQGHLLIPFLYATDMMEWEDVSYGWALFAVYGA
                     CQVAITFAVCVPLERWRPVECWPDARAVTVDVLYTIISRVGILPLFTFVLFYRAQVSL
                     NGFLADHGYVPPMLERVFPFLLGQPVLTFIIYAIILDCADYWRHRLSHMFYWWWALHS
                     LHHAQRQMTFWSDDRNHILDDVISALWFGAIALAIGVPPLQFPLIVLLLRFLESLSHA
                     NARVSFGWFGERLLISPRFHRAHHGLLAAGQRSCNYGAVLPWWDTLFGTADFSREYVA
                     TGDPTGEEALASGSYIAQQWAGLRRFIRVVGRSGRARNAH"
     gene            complement(4457..>5012)
                     /locus_tag="VKQ27_10845"
     CDS             complement(4457..>5012)
                     /locus_tag="VKQ27_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HLY89469.1"
                     /translation="FPYPLRDLLGDEAHAAAYRDGSYVTLRLTSSMYHRFHAPHHCHV
                     EAVTYIAGDTWNVNPIALKRVERLFCKNERAAIRVRLDQTEHRLTLVAVAAILVAGIR
                     VNFLDGSNDVRLGGPRTTWLDAPMMKGQEMGWFQHGSTIIVFAPQGFTLAENVRQGQT
                     IRVGQPLMRIPGSARPTDDADKSP"
BASE COUNT          794 a         1543 c         1646 g         1029 t
ORIGIN      
        1 gggtaggtag ctgagccaga gttacccggt gctttcacgg ggcggagagc cgctggcccc
       61 agcccgcgaa attcgcgcta tgccgcgtgt gcgaggcgca agccgggctc gggccactcc
      121 atcgcgacca gctggccggt gccggacagc gtgatgtacg ccgtgcgccg gtccgggcca
      181 ccgaagcaga tattggtggt caccgggtcg cccatcggca cctggcgcat caggcgtcca
      241 tcaggtgcga tcacgctgat gcagcccgtg atcagtgtgg ccacgcatat gttgccggac
      301 gcgtcgacgg ccaggctgtc gaagcgctgc aaaccgggca gcccgatcac cagccggccg
      361 ccgtgcggcg agggccagcc ctgcttagcg accacccctg gcgaagtgat gtcgaacgcc
      421 cacacccgcg caggctcggt ttcggcgacg tagagcaccg tgccgtcggg cgacaggccg
      481 atgccgttgg gtgtcagggc cggataggca acctcgacaa tgcgggaacc gtcggccagc
      541 gcatagtaga tgacgccgtt gtcgcgatcg cgcgcgcggg tcttgccgag gtcggtgaaa
      601 tagaaccccc cgagcgcgtc gaacacaatg tcattcggac cgttgagttt gatggcgcca
      661 cagcggtcgt agaggacgct gacagaacct gtgcgggcat cgacccgctg gatgcggccg
      721 gtcacgtagt ccgacgcgac cattgccggg cggaacaggt tttcctccag ccgccaggcg
      781 aagccgccgt tgttgcagac gtagaaggcg ccgtccggtc cgattgccag gccgttcggc
      841 ccgccgcccg tatgagcgat cacctgcacg gagccatcgg gcaacacgcg actgacggtc
      901 tggcgctcga tctccaccag cacgaccgag ccatcggcca tcgccaccgg gccttcgggg
      961 aagcatagcc ccgtggccat cacgcgcagt tcactcatcc gtccgttccc ccgcgacagt
     1021 ttgttgaagc ctagccgaat tactggcgga aaaaagcggt ttggtaggga tttcatgctc
     1081 cgatagaggc atgcgattgc cgctgccgac gccgtggtcg ggccggttcg atcagggcac
     1141 gccgctgctg ctcatggttg gtgcggcgtt cgccgcctct gcggcggtgg cggtaatggt
     1201 gccgaggtgg ttctggctgc cacttggcat catcgcagtg ggcggtattg ggatactggc
     1261 ctatcgccat acgatcgctt tctgcgttgc ctggctgctg atagtcggcg cgacgctgga
     1321 gatggcattg gccgatacca tcgggcttgg cctgtatcag ccggtgatcg ccgccgtgaa
     1381 ggcgaccgaa atcctgctcg ccgcgctgtg tgtgctgcgc tatggcccat actcggatgt
     1441 gttcaaccca ggattggcct tcctggcgat gttcgggttc gggctggtcc atggcctgca
     1501 ccccggcctg acgccggtcg acagtctccg ctcgctgatt ggctcgatag cgccgttcgc
     1561 gttcgggttc agccggcttt ccgctcgctg ggcgcgcgcc atcgtcaggg cgacaatttg
     1621 gatcccgctg ttgtcggtgg cgggaggcgc ggctctgtcg ctggccggcc tgcgcgatct
     1681 tttcgtggag agcggcggcg aacggctggc tggtctcggc catccggcgt tcctggccgg
     1741 gttctgcctc gctgcgatct atggctgcct gatcgaactg tatcgggagg gcctgtcgcg
     1801 ctggatgata ctgctggctg tcaacttcgc cattctcgtg ttgactggcg cgcgggcgcc
     1861 gatggcttac ggggtcgcgg tcactgggct gactttggtc ttcgtccggt cagacgcatt
     1921 cccgcgccgt tgccgcattc tgccgctgct gctggctgcc tgcctgttgc cgttactgtt
     1981 ggtgctggcc gggcacctga ccgaggtacg cctcttcaac gtgctgacca ccgaggccgc
     2041 taatctgagc ggccgcgaac tgctgtggcc accgttccag cggtcggccg atgcgtcgcc
     2101 gtggttcggc tggggcgtcg gcgcaggcaa cgcgatcatc ccgcccgata gcgagcttgc
     2161 gcgcataatt caaagctggg cagcgcacaa cgagtacctg cggatgtcgg tcgagggggg
     2221 ccagattggc cgcggtctgc tgatcgccct gtttgtatta tgggtcgtgc atcacacgcg
     2281 tgttctgtgt cgcaccgacc gtgctatcat gcgcctcgtg ttcctggcat tcgccgcaca
     2341 cgcctacacc gacaacgtgc tgatctccac gactgcctgt gtgttctttg ctttcgtcac
     2401 cgcagtgttc gcgcgcggac gcttgatgaa cacggcagag gtggcgtagg caactagcgg
     2461 attttgccgg agccggtgca tgttacgcgc ctttctgctg ctgctgccga tggcgctgtg
     2521 ggccaacgtg gcgttcgccg ccggccttgc gtacgtggtg aactcaggcg ctgcatcgat
     2581 cagcctgatc gagatgtcga ccgagaagga actgcgggtg atcccgctgc tgcgcgagcc
     2641 gcatcacctg gcgctcagtc ctgacgggaa gagcctgctg gtgggcgata ccgttggcaa
     2701 cgaaatgttc ttcttcgatc cggcgacggc agcgttgcag aagcgcttac cggtggccga
     2761 cccgtaccag cttgcattca gtcccaatgg gaagtttctt gtggtcaacg ggctcgcccg
     2821 gaatcaggtc gatgtctacg acgcggcaag catgcagttg ctgaagcgct ttccgatcgc
     2881 gtcgatgccg agccacctgg attacgcacc ggactcatca cgcgtgttcg tgagtttgca
     2941 aggtacggac agcctggtgg cgatcgacct gaacaagctc gaggtgctgt ggaaggtgaa
     3001 gatcggcagc acgccagccg gggtgatgtg gcacaacggc aaggtgttgg tggccgatat
     3061 ggggaccgac tacgtcgctg tgaccgagcc ttcggatggg cgcgtggttg agcgtatcca
     3121 cacggggaag ggggcgcata acctgttcct gtcgccggat cgcaagatca tctgggtgaa
     3181 caaccgtgtg ggtgggacga ccacctcggt cgatgcaacg acgttggcag tgatccgcac
     3241 gtaccggatt cctggcggcc cggacgatat tgcctttgcg ccggatggga agctgtggat
     3301 cacgcgacgc tgggcggaga aggtggcggt gctcgacccg cagacgggcg actaccagac
     3361 gatcgacgta ggccgctcgc cgcatgggat cttcctgtat ccgaagggcg acgcaggtcg
     3421 cagcgtggcg gcacgctgac gttgttctag aatcacggag acacaccgat ggatacagat
     3481 aagcccatcg tacacttgca aagctccaga ttttatctgt gtttatccgt gtccatttgt
     3541 ggttcaatat ccttggttcc acatgcctga ccccttcgac ctcactgccg gctggcttca
     3601 aggccatctg ctgatcccgt tcctgtacgc taccgacatg atggaatggg aggacgtgtc
     3661 ttacggttgg gcactgttcg ccgtttatgg cgcctgccag gtggcgatca cctttgccgt
     3721 gtgcgtgcca ctggaacgct ggcgtccagt cgagtgctgg ccggatgcgc gcgccgtcac
     3781 ggtggacgtg ctgtacacga tcatctcgcg ggtcgggatc ctgccgctgt tcaccttcgt
     3841 gctgttctat cgtgcgcagg tatcgctgaa cggcttcctg gccgaccacg gctacgtgcc
     3901 gccgatgctg gagcgggtat tcccatttct gctcggccag cccgtgctga ccttcatcat
     3961 ctacgcgata atcctcgact gcgccgacta ctggcggcat cggctgtcgc acatgttcta
     4021 ctggtggtgg gcgctgcact cgctgcatca cgcgcagcgc cagatgacgt tctggtctga
     4081 tgatcgcaac cacatcctgg acgatgtgat cagcgcgctt tggtttggcg cgattgcgct
     4141 ggcgatcggc gtgccgccgt tgcagttccc tcttatcgta ttgctgctgc gctttctgga
     4201 aagcctgtcg cacgccaatg cgcgggtgtc attcggctgg tttggcgaac gcttgctgat
     4261 ctcgccgcgg ttccatcgcg cgcatcacgg gctgctggct gcggggcagc ggagctgcaa
     4321 ctatggcgcc gtcctaccgt ggtgggacac gctgttcggc accgcggact tctctcgtga
     4381 gtacgtggcg accggtgatc cgacaggcga ggaggcgctg gcgagcggat cctacatcgc
     4441 gcagcaatgg gcggggctac ggcgatttat ccgcgtcgtc ggtcggtcgg gccgagccag
     4501 gaatgcgcat tagcggttgc ccgacgcgga tcgtctgacc ctgcctcacg ttctccgcca
     4561 acgtgaatcc ctgtggcgca aacacgatga tggtggagcc gtgttggaac caccccattt
     4621 cctggccctt catcattggc gcgtcgagcc acgtggtgcg cggtccaccc aggcgcacat
     4681 cgttgctgcc gtccaggaag ttgacccgga tgcctgccac caggatcgcc gcaaccgcca
     4741 ccagtgtcag ccgatgctcc gtctgatcca accgcacccg gattgctgca cgctcattct
     4801 tgcagaacag ccgctccacc cgcttgagcg cgatcggatt gacgttccag gtatcgccgg
     4861 cgatgtaagt gacagcctcc acgtggcagt gatgcggcgc gtggaaccga tgatacatcg
     4921 atgaggtcag ccgcagcgtc acgtaggacc cgtcgcgata tgccgcggcg tgcgcctcat
     4981 caccgagcaa gtcacgcagc ggatagggga ac
//
LOCUS       DATGEM010000340         9072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_95171, whole genome shotgun sequence.
ACCESSION   DATGEM010000340 DATGEM010000000
VERSION     DATGEM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95171"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..673)
                     /locus_tag="VKQ27_10850"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="VKQ27_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631516.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
     gene            complement(722..1222)
                     /locus_tag="VKQ27_10855"
     CDS             complement(722..1222)
                     /locus_tag="VKQ27_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89470.1"
                     /translation="MHAHHPKPNDSKVSGIGTVLLGVAAIALAGMVGTIGLIRQVGTL
                     GPKVGDIVAFDPQDTMSRDIKPHVIAVPADQIGMACELDVQAMHQGGGSLIIESRRLE
                     PGGGIRIHWSGARTATDGSNCGSSADLVVEQDDIEILALAAGGFGPSAAKHASGMLWS
                     RNTPMQ"
     gene            complement(1487..2881)
                     /gene="gltX"
                     /locus_tag="VKQ27_10860"
     CDS             complement(1487..2881)
                     /gene="gltX"
                     /locus_tag="VKQ27_10860"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLY89471.1"
                     /translation="MSVRVRFAPSPTGPLHIGSARTALFNYLFARHHRGEFLLRIEDT
                     DRERSTEASTKGILDSLDWLGLPRDGQTVFQSTRQARHVEVAHALLATGHAYYCFCTP
                     EELQRERETARAEGRVWRYDGRWRDRDPATAPPGVKPVIRLKAPREGDTVVHDLVQGN
                     VRVANSELDDMIILRSDGVPVYNHSVVVDDHDMGITHVIRGDDHLTNTFRQVQIYQAM
                     EWELPRFAHLPLIHGPDGTKLSKRHGAQFVLEFDEQGYLPEALNNFLLRLGWGHGDVE
                     LLDREEAIRLFDLDGVGRAPGRMDYAKLSYLNGVYIRQADDERLTRDVLRRLARRTDL
                     ALGSKAAQRIRTLMPALKERAKTLVELTDAATFLARALPLPLEPKAAAILTPEARLML
                     RDVATALAASDFSPPGIDAALRAFAESTGRKLGQVAQPLRAALTGSTASPGIDLTLAA
                     LGKEEALARLAAVA"
     gene            2942..5125
                     /locus_tag="VKQ27_10865"
     CDS             2942..5125
                     /locus_tag="VKQ27_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLY89472.1"
                     /translation="MRQKFADWLDAWRAAEQGHFALWLPVFMGAGVLLYFGLRSEPWA
                     WLGASVAVPAVAGAVLARSWPVTRAALAALAAGAVGFTAAQFATATVGPLDPLPNHAA
                     IYTGTVRGVEAVPTGRRVTLDTVTIEGEPAPLHRRLRVRLKANDVTVVTSGDIVRVRA
                     LVRPASPPAYPGAWDMQRDVFYSDLSGMGYALGPVDLRSHAPDSAMLWILRLRESINR
                     RIDAVLGGGVAALAQALLAGVMSGVPPADMAAFRDSGLAHLLSVSGLHIAIVMGIGVT
                     VVRLSLAAWPYAALRWPCKHIAVLAGLGAGAFYMVLTGSQVPMVRSFLMAAFVGVAML
                     AGRRALSMRTLVVAAALLVLVAPDALIGASMQMSFAAVMALIAGNEAFGGRLSALYRR
                     HGWGGKAAAYVLGLVLTSLLAGSATLPFGAYHFGRVQFYFALSNLVAVPLTGIVVMPA
                     GMLSLALMPLGWEWPALVVMGWGIEGVLWIAHAAAALPGATWMAPHMPPWGILIVAFG
                     MVWLSLWRSRLRLFGIAVMLAGLSSPLLERPPDILVSAEARLIAVRTPAGAFVQQISG
                     ASKFTREAWLRYWGEPAFQPIPARGTSANGAIDCSQPVCVLRPRAGARPALLVRGSAR
                     PDGCGDAAVLVAAEPARRLCPRPWPALVDRFTVWRYGAAAIWLSAGRARVLTDRAYRG
                     DRPWVPPVPTPRGRIAPGLAPAERDVVPAPAAPPPQQEASGAVMR"
     gene            complement(5122..5844)
                     /gene="lexA"
                     /locus_tag="VKQ27_10870"
     CDS             complement(5122..5844)
                     /gene="lexA"
                     /locus_tag="VKQ27_10870"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLY89473.1"
                     /translation="MLTRKQYELLMYIDHHLKQTGFSPSFEEMKDALKLKSKSGIHRL
                     ISALEERGFLGRRRHRARALEVLRLPENLGARGNGEAALVQEPAQTFAPNVIRGDFKP
                     KISGVQAANDAEAVQLPLYGRIAAGLPIEALQDPTSFVGTPMSLLGNGEHYALEIAGD
                     SMVEAGILDGDTVIIRKSDTADTGQIVVALVDENEVTLKRLRRRGNSIALEPCNVRHE
                     TRIFPADRVKVQGKLVALMRRY"
     gene            complement(5962..7167)
                     /gene="glp"
                     /locus_tag="VKQ27_10875"
     CDS             complement(5962..7167)
                     /gene="glp"
                     /locus_tag="VKQ27_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HLY89474.1"
                     /translation="MISVEEARDRILATLHPTPAEIAALADAWGRVTATPVAARLTQP
                     PTDVSAMDGYALRAADGTLGATLHVIGAAPAGHPFDRPVGPGQAVRLFTGSVVPQGAD
                     GILLQEDATAIGTEVRINEAVVAGRHIRRAGQDFAAGDTVVPAGRRLTARDIGLAAAA
                     NHPWLTVHRRPRVAILATGDEIAMPGEPIPSGGIVSSNSHALAALVRASGGEPVVLPV
                     AADTREAVAAVADSIHGMDMLVTTGGASVGDHDLVIESLKSRGLELDFWQIAMRPGKP
                     LLYGKLGAVPVLGLPGNPVSALVCSILFLLPALNRLQGLPAAPPPTIQAIAGAPLKAN
                     DHRADHLRSAITADSLGRVVATPFPVQDSAMLRRLARADALILRAPNAPALPQGAEIS
                     VIPLDSLGI"
     gene            complement(7178..8194)
                     /locus_tag="VKQ27_10880"
     CDS             complement(7178..8194)
                     /locus_tag="VKQ27_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HLY89475.1"
                     /translation="MGDRILIWGAGAIGGSVGAWLKRAGHDITFVDVVPDHVTAIRTT
                     GLRITGPVEEFTVTTPALLPEEVTGIWPRIFLAVKAHHTEAATRSLTPHLAQNGYVLS
                     LQNGLNEHVIQRIVGRDRTIGAFINFGADWIAPGEILFGGRGAVVLGELDGVITPRLE
                     ALHAIMRDFEPDAITTSDIWSYLWGKLGYGAMLFAQGLGRMSIADCLARPELLQLWRR
                     IGEEAITVAEAEGVRPRGFNGYDIDAFRRGATEDAARASVAAMVSFNSTSAKTHSGIW
                     RDLAVRKRRTEVDVQIAPIADIGALHGIDCRVTRRLVEMVHEVEEGRRAMTDDNLLEL
                     ARVN"
     gene            complement(8194..8889)
                     /locus_tag="VKQ27_10885"
     CDS             complement(8194..8889)
                     /locus_tag="VKQ27_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLY89476.1"
                     /translation="MRIAEMDWRMVEDWVRHDDRCALPLGSTEQHAGLSLATDAILAE
                     RVAAEAAEPLGIPVFPALPFGLTPYFTAFPGTVTLRVATYAAVLRDVLDSLKHAGFRR
                     VLIVNGHGGNAPAGGIAQEWMMDNPDARVRYHEWWRAPLTAAAVRAIDPVASHASWME
                     NFPWTRLANRPPQEGIKRMIDTDRLRMLPPFEVRTLIDDGNFGGRYQRDDSEMLELWD
                     VAVRETRAMLESW"
BASE COUNT         1378 a         2711 c         3392 g         1591 t
ORIGIN      
        1 ctggagacgc agcatcatga cgatgtcagc gccggcgagg cctcgccatg tcatggaaga
       61 cttcgacgcc gaggcgttcg gcttcggccg gcatcagcgt gggtgggccg acgacgcgga
      121 cccggctgcc catggtggtg agcaggtgga tgttcgagcg ggcgacccgg gagtgcagca
      181 cgtcgccgca gatggcgacg atgaggcctt cgagccggcc cttgcggcgc ttgatggtga
      241 gggcgtcgag cagcgcctgg gtggggtgct cgtgcgttcc gtcgccggcg ttgatcactg
      301 cggcatcgac cttctgggcg agcaggttgg gggcgccgga ctggtcgtgc cgcaccacca
      361 gcaggtcaca gtgcatcgcg ttgagcgtgg cggcggtgtc gagcagggtc tcgcccttgt
      421 tcaccgagga ggtggagaca gacatgttga tcacgtcggc acccagccgc ttgccggcaa
      481 gttcgaagct ggtacgggtg cgggtcgagt cctcgaagaa caggttgatc agcgtgcggc
      541 cgcgcagcag gtcgcgctgg gttttgccgg aacggttgag cagaacgtag ctctcggcga
      601 ggtcgagcaa atgcccgatc tcgggggggt agaggccctc gatcgccagg agatgctttt
      661 tccggtacga cacgaccctt ggtcgcagaa agcggccgcg gccgtcaacg cccggttcgg
      721 gtcactgcat cggggtgttt cggctccaca acatgccgct cgcatgcttt gccgcgctgg
      781 ggccgaaacc gccggccgca agtgccagaa tctcgatatc gtcctgttcg accacgaggt
      841 ccgccgaact gccgcaattg ctgccgtcgg tcgcggtgcg cgcgcctgac cagtggatcc
      901 ggatgccccc gccgggctcc aggcggcgcg actcgatgat caagctgcca ccgccctgat
      961 gcatggcctg gacatcaagc tcgcaggcca tgccgatctg atcggccggg accgcaatga
     1021 catgaggctt gatatcgcgc gacatcgtgt cctgcgggtc gaaggcgacg atgtcgccga
     1081 ctttgggacc gagggtcccg acctgccgga tcaagccgat cgtgcctacc atgccggcga
     1141 gcgcaatcgc tgccacaccg agaaggacgg tgccgattcc gctgaccttg ctgtcatttg
     1201 gtttcgggtg atgtgcatgc atgactggtt ttccacgttg tcgtccggga cgcagtatcg
     1261 cccggcgtgg ttcatatttg atgaagtcaa ctgtgagata gaatcagtaa atatactgat
     1321 tataaagaaa aatggcggca cctaggggtg ccgccgaggg tatggggagg agtccaagag
     1381 agaggagagc acgggtcgga acccgtccgc ctcacagacg gcagattgcc cggcgggcga
     1441 cggaccagca ttgatccaga tcaagggtgc gtaatttttc ttcgcttcag gcgacggcgg
     1501 cgaggcgagc gagcgcctct tccttgccca gggccgccag ggtcagatcg atccccgggc
     1561 ttgccgtgct gccggtcagc gcggcacgca gcggctgcgc aacctggccg agtttgcgac
     1621 cagtgctttc ggcgaaggct cgtagcgccg cgtcaatccc gggcggcgag aagtcgctcg
     1681 cggccagcgc ggtggcaacg tcgcgcagca tcagccgtgc ctcgggcgtc aggatcgcgg
     1741 ccgcttttgg ttccagcggc agaggtaggg cgcgggcaag aaatgtcgct gcatcagtga
     1801 gttccaccaa agtcttggcg cgctccttga gtgccggcat caaggtacgg atgcgttgcg
     1861 cggctttgct gccgagagcc agatccgtgc ggcgggccag ccgtcgcagc acgtcgcggg
     1921 tcagccgctc gtcgtctgcc tgccggatgt agacaccgtt caggtaggaa agcttggcgt
     1981 agtccatccg ccccggggcg cggccgacac catccagatc gaacagccgg atggcctcct
     2041 cgcggtcgag gagctcgaca tcgccatgac cccagccgag gcgcagcaga aaattgttca
     2101 gcgcttcagg cagatagccc tgttcgtcga attccagcac gaactgcgca ccgtgccgtt
     2161 tcgagagctt ggtgccgtcg ggaccatgga tcagcggcaa gtgggcgaag cggggcagtt
     2221 cccactccat ggcctggtag atctggacct ggcggaaggt attggtgagg tgatcgtcgc
     2281 cgcggatcac gtgggttatg cccatatcgt ggtcgtccac cacgacggag tggttgtaga
     2341 cgggcacccc atcgctgcgc aggatgatca tgtcgtccaa ctcggagttg gcgacgcgga
     2401 cgttgccctg cacgaggtcg tggacgacgg tgtcgccctc gcgaggggcc ttgaggcgga
     2461 ttaccggctt cacgccgggt ggggcggttg ccggatcgcg gtcgcgccag cggccgtcgt
     2521 agcgccagac gcggccctcg gcgcgggcgg tctcgcgttc ccgctgcagt tcctcggggg
     2581 tgcagaagca gtagtaggcg tggccggtgg ccagaagggc gtgcgccacc tcgacgtgcc
     2641 gcgcctggcg ggtggactgg aacacagtct ggccgtcacg cggcaggccg agccagtcga
     2701 ggctgtcgag gatgcccttg gtcgatgcct cggtgctccg ttcgcggtcg gtgtcctcga
     2761 tgcggagcag gaattcgccg cggtgatggc gggcgaacag gtagttgaac agtgcggtgc
     2821 gggcgctgcc gatatgcagc ggcccggtcg ggctgggggc gaagcggacg cgaacggaca
     2881 tgcacatgat ctagcacgca cattcaccgg ctgtagaggc gagggcggta acctcccatg
     2941 catgcggcag aaattcgcgg actggctgga cgcgtggcga gccgcggagc aggggcactt
     3001 tgcgctgtgg ttgcctgtgt tcatgggcgc gggggtgctg ctttacttcg ggcttcggtc
     3061 cgaaccatgg gcatggctcg gtgcttctgt cgcagtccct gccgtcgcgg gcgccgtgct
     3121 ggcgcggtct tggccggtta cgcgcgccgc tctggcggct ttagcggccg gcgccgtcgg
     3181 tttcaccgcg gcgcagtttg ccacggccac ggttgggccg ctcgatccgc tgccgaacca
     3241 tgcggctatt tacaccggca cagtccgcgg cgttgaggca gtgccgacgg ggcgccgggt
     3301 cacgctcgat acggtgacaa tcgaagggga gccggcaccg ctgcaccggc ggctgcgcgt
     3361 gcggctgaag gcgaacgatg tcaccgtggt gacgagcggc gatattgtcc gcgtccgcgc
     3421 gctggtgcgg ccggcgtcgc caccggccta tcccggcgcc tgggacatgc agcgcgacgt
     3481 gttctactcc gacctctcgg gcatgggata cgcgctgggg ccggtcgatc tgcgcagcca
     3541 cgctcccgac agcgccatgc tgtggatcct gcggctgcgc gagtcgatca accgccggat
     3601 cgacgcggtg ctgggcggcg gcgtggcggc gttggcgcag gcactgctcg ccggcgtgat
     3661 gtccggtgtg ccgccggccg atatggcagc ctttcgtgat tccggcctgg cacatctgct
     3721 gtcggtgtcg ggcctgcaca tcgcgatcgt gatgggcatc ggtgtgacag tggtgcgcct
     3781 gtcgcttgcc gcgtggccgt atgccgcgct gcgatggccc tgcaaacaca ttgcggtgct
     3841 ggcgggcctg ggggccggcg cgttctacat ggtgctgacc ggctcgcagg tgccaatggt
     3901 gcgcagcttc ctgatggcgg cattcgtcgg ggtggcgatg ctcgccggca ggcgggcgtt
     3961 gtcgatgcgc accctggtcg ttgcggcagc gctgctggtg ctggtggcgc ccgacgcgct
     4021 gatcggcgcc tcgatgcaga tgagctttgc cgcggtgatg gcgctgatcg cgggcaatga
     4081 agcattcgga gggcgactga gcgcgctgta ccgccgccac ggctggggcg gcaaggctgc
     4141 cgcctatgtg ctggggctgg tactgaccag cctgctggcc ggcagcgcca cattgccatt
     4201 cggcgcctac catttcgggc gcgtgcagtt ctatttcgcg ctgtcgaacc tggtcgccgt
     4261 gccgctgaca ggcatcgtgg tgatgccggc cggtatgctg agcctggcgc tgatgccgtt
     4321 gggctgggag tggccggcgc tcgtggtcat ggggtggggc atcgagggcg tgctgtggat
     4381 cgcccacgcc gctgctgctc tgccgggcgc gacatggatg gcgccgcata tgccgccatg
     4441 ggggattctc atcgtggcct tcggcatggt ctggctgtcg ctgtggcgga gccggctgcg
     4501 cctgttcggc atcgcggtca tgctggccgg gctgtcgtcg ccgctgctgg agcgaccccc
     4561 ggacatcctg gtgtcggccg aagcgcggct gatcgcggtg cgcacgcccg ccggagcgtt
     4621 tgttcagcag atttcagggg catcgaagtt cacccgggaa gcctggcttc gctattgggg
     4681 cgaaccggcc ttccagccga tcccggcgcg gggcacgtcg gcgaatggcg cgattgactg
     4741 cagccagccg gtctgcgtgc tgcggcccag ggcaggtgcg cggccggcat tgctggtgcg
     4801 gggttcggca cgtccggacg ggtgtggcga cgcggcggtg ctggttgccg ccgagcctgc
     4861 tcgcagactg tgtccacggc cctggccggc gctggtcgac cggttcacgg tctggcgcta
     4921 cggggcggcg gccatttggc tgagcgcagg acgcgcccgg gtgctaacag atcgggcgta
     4981 tcggggcgat cggccgtggg tgcctccggt gccgacgcct cgggggcgga ttgcgccagg
     5041 cctggcgcca gcggagcggg atgtcgtgcc ggcgcctgcc gctcccccgc cgcaacagga
     5101 agcgagcggt gcggtgatgc gttagtaccg gcgcatcagt gcgacgagct tgccctggac
     5161 cttgacccga tcagccggga agatgcgggt ttcgtggcgg acgttgcagg gctcgagggc
     5221 gatcgaattg ccgcggcggc gcaggcgctt cagggtcacc tcgttctcgt cgacgagggc
     5281 aacgacaatc tggccggtgt cggcggtatc gcttttgcgg atgatgacgg tgtcgccgtc
     5341 gaggatgccc gcctcgacca tcgaatcgcc ggcgatctcc agggcgtaat gttccccgtt
     5401 gcccaagagc gacattggcg tgccgacgaa gctggttgga tcctgcaacg cctcgatcgg
     5461 caaaccggcg gcgatacggc catagagcgg caactggact gcttcggcgt cgttggcggc
     5521 ctggacaccg gagatcttag gcttgaagtc gccgcggatg acgttggggg cgaaggtctg
     5581 ggcaggctcc tgcacgagtg cggcctcgcc attgccgcga gcaccgaggt tctccggcag
     5641 gcgcaggact tcgagggcgc gagcacggtg gcggcggcgg ccgagaaagc cgcgttcctc
     5701 gagtgcggag atcagccggt ggataccgga cttggatttc agtttcagcg cgtccttcat
     5761 ctcctcgaag ctcggcgaga agccggtctg cttcaggtga tggtcgatgt acatcagcag
     5821 ttcgtactgc ttgcgcgtga gcatggcggg cgttctttcc cctctgggcg cgctcggcag
     5881 cgcgccggaa caaaccggca actgattggt tgttctagat aggttcgcgc cgcgtcgtca
     5941 agaattccta aacgttcgcg ctcaaattcc gagtgaatct aggggaatga cgctgatttc
     6001 cgcgccctga ggcagggccg gggcattggg cgcgcgcaaa atcagcgcgt cggcgcgcgc
     6061 caggcgcctt aacatcgctg aatcctgcac ggggaagggg gtcgcgacca cgcggccaag
     6121 cgaatcggcg gtgatggcgg aacgcagatg gtccgcgcgg tggtcgttcg ctttgagtgg
     6181 cgcccccgcg atggcctgaa ttgtcggcgg cggcgcggcg ggaaggccct gaaggcggtt
     6241 cagggcaggc agcaggaaca ggatcgagca gaccagggcg gaaaccgggt tgccgggcag
     6301 accgaggacc ggcactgcgc cgagcttgcc gtagagcagc ggtttcccgg ggcgcatcgc
     6361 gatctgccag aaatccagtt ccagtccgcg gctcttcagg ctttcaatga ccaggtcgtg
     6421 gtcgcccacc gaggcgccgc cggtggtgac cagcatgtcc atgccgtgga tggaatcggc
     6481 gacggcggcg acggcctcgc gtgtgtcggc ggcgacgggg agcacgaccg gttcgccgcc
     6541 ggaggcgcgg accaaggcag cgagggcgtg cgagttggag ctgacgatgc cgccggaggg
     6601 gatcggttcg ccgggcatgg cgatctcgtc cccggtggcg aggatggcga cgcgcggacg
     6661 gcggtggacg gtcagccagg gatggttggc ggctgcggcg aggccaatgt cgcgcgcggt
     6721 gaggcggcgg ccggccggaa cgacggtgtc gccggcggcg aagtcctggc cggcgcggcg
     6781 gatgtggcgg ccggcaacca cggcctcgtt gatgcggact tcggtgccga tggcggtggc
     6841 gtcttcctgg aggaggatgc cgtcggcacc ttgagggacg acgctgccgg tgaacaggcg
     6901 gacggcctgg ccgggaccga cgggacgatc aaaggggtgg ccggcgggag ccgcgccaat
     6961 gacgtggagc gtggcaccga gcgtgccgtc ggcggcgcga agggcgtagc cgtccatggc
     7021 ggagacgtcg gtggggggct gggtgagacg ggcggcgaca ggggtggcgg tgacgcggcc
     7081 ccaggcatcg gcgagggcgg cgatttccgc gggcgtggga tggagcgtgg cgaggatgcg
     7141 gtccctggct tcttcgacgg agatcatcga ggctctccta gttcaccctg gccaattcga
     7201 gcaggttgtc gtcggtcatc gcgcggcggc cttcttccac ttcgtggacc atctccacca
     7261 gcctgcgggt gacccggcag tcgatgccgt gaagggcgcc gatgtcggcg atgggtgcga
     7321 tctgaacatc tacctcggtg cggcgcttgc gcacggcaag atcgcgccag atgccggaat
     7381 gtgtcttcgc gctggtgctg ttgaatgaaa ccatcgcggc gacactggcg cgggcggcgt
     7441 cctcggtggc gccgcgcctg aaggcgtcga tgtcgtagcc gttgaagccg cgcgggcgaa
     7501 cgccctcggc ctcggcgacg gtgatcgcct cttcgccgat gcggcgccaa agctgcagca
     7561 gttccgggcg tgcgaggcag tcagcgatgc tcatccggcc gaggccttgg gcgaacagca
     7621 tcgcgccgta gccgagcttg ccccagagat aggaccagat atcgctggtg gtgatcgcgt
     7681 ccggctcgaa gtcgcgcatg atggcgtgga gtgcttcgag gcgcggggtg atgacaccgt
     7741 cgagttcgcc gagcacgacg gcgccgcggc cgccgaacag gatctcgccg ggcgcgatcc
     7801 agtcggcgcc gaagttgatg aaggcgccga tggtgcggtc gcggccgacg atgcgctgga
     7861 tgacgtgttc attgaggccg ttctgcagcg acaggacgta accgttctgg gccagatggg
     7921 gcgtgagcga gcgggtggca gcttcggtat gatgggcttt cactgcgagg aagatgcgcg
     7981 gccagatgcc ggtgacttcc tcgggcagca gggcaggggt ggtgacggtg aactcttcca
     8041 ccgggccggt gatgcgcagg cctgttgtgc ggatggcggt gacgtggtcg ggaacgacgt
     8101 cgacgaacgt gatgtcgtgg ccggcgcgtt tcagccatgc gccaaccgag ccgccgattg
     8161 cgccggcgcc ccagatgagg atgcgatcgc ccattaccag ctttccagca tggcgcgcgt
     8221 ttcccgcacg gcgacgtccc acaattccag catttccgaa tcgtcgcgct ggtagcggcc
     8281 gccgaagttg ccgtcatcga tcagggtgcg gacttcgaag ggggggagca tgcgcaggcg
     8341 atcggtgtcg atcatgcgct tgatgccttc ctgcggggga cggttggcga ggcgcgtcca
     8401 ggggaaattc tccatccagc tagcatggct ggcgacagga tcgatggcgc gcacggccgc
     8461 ggcggtgaga ggcgcgcgcc accattcgtg gtagcggacg cgcgcgtccg gattgtccat
     8521 catccattcc tgcgcgatgc cgccggctgg cgcattgccg ccatggccgt tgacgatcag
     8581 gacgcggcgg aagccggcgt gcttcaggct gtccagcaca tcgcgcagca cggcggcata
     8641 ggtcgcaacg cgcagcgtga cggtgccggg gaaagcggtg aaatagggcg tcaagccgaa
     8701 ggggagggcc gggaagaccg gtatgccaag cggttcggca gcttcggcgg cgacgcgttc
     8761 ggcaaggatc gcatcagtgg cgagcgacag gccggcgtgc tgttcagtgc tgcccagtgg
     8821 caaggcgcag cgatcgtcgt ggcggaccca gtcttccacc atgcgccagt ccatctcggc
     8881 tattcgcatg tccggtgtgc ctcgtgctgc ggttggatcg cacgctggac agagttggtg
     8941 agggcgtcaa ggaagggttc acagggggac cgcggagaag caatgaggac cacggagaaa
     9001 ttcgatgcgc gctacgcgcg cttcagacat gacgatttat accgaagccc aatgacggaa
     9061 gctcattgct cg
//
LOCUS       DATGEM010000341         7222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_123639, whole genome shotgun sequence.
ACCESSION   DATGEM010000341 DATGEM010000000
VERSION     DATGEM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123639"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="dctP"
                     /locus_tag="VKQ27_10890"
     CDS             complement(<1..911)
                     /gene="dctP"
                     /locus_tag="VKQ27_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HLY89477.1"
                     /translation="MSQGISRRALTRASVLTLGSFAILRHARGDAPIELRCSLDTAPS
                     HPRNVAFREFLSKIEQESGGQIKTKLFESGSLFPDLQVLKALVQGQVEMACPGTWTIT
                     GFVPDADFSQLPAFYGRPAEVVHKVVNGTAGKFVNDEIHNKLHVQVLGGWFDLGFNEW
                     FSTRKPLESLASLKDMKLRSPGGVLNSFRIRYFGGIPNVTAWPDVPLAMSQGTFDGLI
                     SSNESVNSSKLFDSGMRHSLQDQQGMGLYVPMANADFWQKIGPKLQEMIQKVWADNLP
                     TWRDNTLKLQANARGVLTKQGVTFVDVP"
     gene            complement(992..1621)
                     /locus_tag="VKQ27_10895"
     CDS             complement(992..1621)
                     /locus_tag="VKQ27_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89478.1"
                     /translation="MTRRSSLLLPLLLAACGGEERRDYPPLRYNYLLPLGLNVAQIRI
                     EQRYMPSGAPPDVSQLDPAPPVDALRNMATDRLQALGAAGTAVFSILNAALIRKDDTL
                     SGTFEVELDVYSAPDTRAAYATASVTGSYTGDLDDIRGRLYDMTRSLMDRMNVEFEYQ
                     VRRSLGGWLLSAGVPQAPVQQQPLIVSPPPDALPPGSPPPGPPPNPQRY"
     gene            complement(1624..2583)
                     /locus_tag="VKQ27_10900"
     CDS             complement(1624..2583)
                     /locus_tag="VKQ27_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HLY89479.1"
                     /translation="MSETPAFLDGDRPPVPDIVVPRGVTPFYLPKRPVRGRLVRLGPL
                     ADALLNRRANHPAVTRLAGQSLALAAALSTALKFRGSFSLQAKGDGPVTMLLADCSDT
                     GALRGYARTKQEKLDALLEADRVPGAGQLLGAGYLAFTVDQGPDQNRQQGIVSIEGGN
                     LAEMALHYFRTSEQLRCFVHLACEQTPDGWRAGALILEKIAGAGGIDPLLDEEAQEES
                     WRTATLLAGTLTDAELLDDNLSPERLLYRLFHSEGVALDRARALSYGCRCSRARLVGI
                     LEGFPPDDLDHMAVDGDIVMTCEFCNYDFRFARDDVHGRSVAS"
     gene            2633..3106
                     /locus_tag="VKQ27_10905"
     CDS             2633..3106
                     /locus_tag="VKQ27_10905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89480.1"
                     /translation="MNFHLRPIVLADATEVAALIRTAFAAQSSLTDPLPSAMRVTEAD
                     VSAHLRTGGGAVAEANGAIVGSALWTEQDGGLYLSRLAVAPAWRGRGIAKALVASGEA
                     AAREKKLPRIHLSTRIPLLDNRRLFAACGFVETTREAHPGYAEPTSVQMEKRLLD"
     gene            3128..4297
                     /locus_tag="VKQ27_10910"
     CDS             3128..4297
                     /locus_tag="VKQ27_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89481.1"
                     /translation="MGSRPWLILAAVALARIGFGYQYQTVASLGPELMHLFQFDYATL
                     GTLIGAFMFFGAFLALPLGLLGRRFGDRLVLGAGLLLMVVGPLISTFSDGPAGIGLGR
                     GLAGIGAVAMIVLQNKIIADWFTGHQLMWALSVSSAAYPVGVGLAQLVLPPLTHSYGW
                     QAGFLSDSVPMAVSLVLFLVSYRASPHAVAQPRTFSLPSARECLLLAIAGLIWTAYTA
                     GYSSYLNYVPSLMAVRGEGLVLTGVVLTIATWGNVPATMLGAGLATRLGAFRIFLFST
                     CALVVGIGGAAVLDWPVTFAVIIGIVGSLHPGVIMAVGTLSARPENRAVGMGLFYSMY
                     YAGGAVVPALCGRAADIYGSPEGALLAASVISVLALPMFLLHRRMAAHETMLARA"
     gene            complement(4218..4754)
                     /locus_tag="VKQ27_10915"
     CDS             complement(4218..4754)
                     /locus_tag="VKQ27_10915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89482.1"
                     /translation="MSTARLARASDLHGLLELFRVSEVSAAAEPLERAERILSETLAR
                     DDVALFVSDANTVVVATCLLITAPNLLRGGRRHGFLENVVTHPDHRGQGHGRAVVQAA
                     LAEAWARDCHHVLLQSGRQDPRVHLFYQSLGFEPGIRVGYAARRPIGIAGPMPAPASF
                     RAPPSGDAAGTSGAPVQK"
     gene            complement(4957..6981)
                     /locus_tag="VKQ27_10920"
     CDS             complement(4957..6981)
                     /locus_tag="VKQ27_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLY89483.1"
                     /translation="MTTAANPDLTETRFVLPPDAKCLQVVELSARLRARIGPVEDDQS
                     VITRPGARVTAKLITATLAALIAEFREPRLITDAVLQFSRTREQDPSAVLDVAFDAIA
                     RLIDGRILVPEDAIDARASEPGLAAGQAFGTFEIDAVMWSLDDTEVYRARSQDGTVVA
                     LKIAREKTAAVAALLGHEATILQRLNGAVTPKLLAHGIEQGRSFIAMEWCEGVSVAIA
                     AQQARAARDRSRLHRLVRAMFEAYSSLHASGVLHGDIHPGNCLVADNGTVTLIDFGRA
                     SLIDAGDEIDPARAGIPQFYDPAMAEALLAGRLPPACTELSEQYSVCVLAYLLLTGRH
                     PIDAPAVQEDLLQRIAARPPLPFATRGIAAWPAVERVLRRGMARQPADRFENMSGLAR
                     SFAATALAATSGPPRSAPELETAIEQTRRLAHSILPPLHRAWFALRAAMALEDAELLA
                     AAAVLIEDAGADWIIQSVATRIAHARSDTRLVRQCFRRFLDSGPPTDRRDTIAAILAS
                     AEALEGTALRDDTAAELGRWASQHLHRLFAEPQQRAAGDDAYLMHAALTLAKAGAVSP
                     QDGLAERLDAIDADEASVWEWAAAYDLFAREDYRQRALDAPRPRQPLARAFALLRLHQ
                     LTGEMQWVAAAHDLLTELAPEQAALLAIELRMPECAVLPSFLLVSNGYDD"
     gene            complement(6978..>7222)
                     /locus_tag="VKQ27_10925"
     CDS             complement(6978..>7222)
                     /locus_tag="VKQ27_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89484.1"
                     /translation="FTSEVGGDGIVSAGVDPPAGNAPTSWRAWVTEKLASALSASPAM
                     NVEDRVGAALAGIRADGVDPCSWSLVDNASARFDAT"
BASE COUNT         1230 a         2419 c         2280 g         1293 t
ORIGIN      
        1 ggcacatcga cgaacgtgac gccctgcttg gtgagcacgc cgcgcgcgtt ggcctgcagc
       61 ttcaaggtgt tgtcgcgcca cgtcggcaga ttatccgccc acaccttctg gatcatctcc
      121 tgcaacttcg gcccgatctt ctgccagaag tcggcgttgg ccatcggcac gtacaggccc
      181 atcccctgct ggtcctgcag cgagtgccgc atgccggaat caaacagctt ggaactgttc
      241 acgctttcat tgctgctgat caggccgtcg aacgtgccct gcgacatcgc gagcggcacg
      301 tcgggccacg cggtcacgtt tgggatgccg ccaaagtagc ggattcggaa tgaattcagt
      361 acgccgccgg gactgcgcag cttcatgtcc ttcagcgacg ccagtgattc gagcggtttg
      421 cgcgtggaga accactcgtt gaagccaaga tcgaaccaac cgcccagcac ctgcacgtgc
      481 agcttgttgt ggatctcgtc gttgacgaac ttgccggcgg tgccgttcac caccttgtgc
      541 acaacttcgg cgggccgtcc ataaaacgcc ggcagctgcg agaagtctgc gtcaggcacg
      601 aagcctgtga tcgtccatgt gccagggcag gccatctcca cctgaccctg taccaacgcc
      661 ttgagtacct gaagatcagg aaatagcgag ccgctctcga acagcttggt cttgatctgc
      721 ccgcccgatt cctgctcgat cttgctcagg aattcacgga acgcaacgtt tcggggatgt
      781 gacggcgcag tatccagcga gcagcgcagc tcgatcggcg catcaccgcg tgcatggcgc
      841 agaatggcaa aactgccgag tgtcaaaact gaagcgcgag tcaatgcgcg tcgcgagatg
      901 ccctgcgaca tggccgtgtc ctccgaaggt ttgcgcggga ttggcgcggg gtatgacgat
      961 gcggcgcccc cgcgtcaagc cgatgacggc gtcaataccg ttgcgggttc ggcggtggcc
     1021 cgggtggtgg cgaaccagga ggcagtgcgt cgggaggcgg cgaaactatc aaaggctgct
     1081 gttgcaccgg cgcctgcggc acgccggccg acaacagcca tccacccagc gaacgacgaa
     1141 cctggtactc gaattcgacg ttcatccgat ccatcaggct cctggtcatg tcatagagcc
     1201 tgccgcggat atcatccaag tcgccagtat aactgccggt gacgcttgcc gttgcgtatg
     1261 cagcacgagt gtcgggcgcc gagtagacgt ccagttccac ctcgaaggtg ccggatagcg
     1321 tatcgtcttt ccggatcagc gccgcattga ggatcgagaa cactgcagtc ccggcagcgc
     1381 cgagcgcctg caaccggtca gtcgccatgt tgcgcagcgc atccaccgga ggtgccggat
     1441 cgagctggct cacatccggc ggcgcgccgg acggcatgta gcgttgctcg atccggatct
     1501 gcgccacgtt cagcccaagc ggcaacagat agttgtaacg aaggggtgga taatcgcgcc
     1561 gctcctcccc gccgcaggcg gcaagcagca atggcagcag caaggacgaa cgtcgggtca
     1621 atatcaagag gcaacactcc ggccatgcac atcatcgcgt gcaaagcgga agtcgtaatt
     1681 gcagaactca caggtcatca cgatgtcgcc gtccactgcc atgtgatcca gatcgtcagg
     1741 cgggaaccct tccaggattc cgacaagccg tgcgcgtgag caccggcagc cgtaggacag
     1801 cgcgcgcgcc cgatccagtg cgacgccttc gctgtggaac agccggtaca ggaggcgctc
     1861 gggtgacagg ttgtcgtcca gcagctcggc atcggtcagc gtgcccgcga gcaacgtagc
     1921 ggttcgccag ctttcttcct gcgcttcttc atccagcagc ggatcaatac cgccggcacc
     1981 ggcgatcttt tccaggatca gcgcaccggc gcgccatccg tccggcgtct gctcgcaggc
     2041 cagatgcacg aagcatcgca actgctcgct ggtgcggaag tagtgcaggg ccatctccgc
     2101 caggttgccg ccctcgatgc tgacgatacc ctgctggcga ttctggtccg gcccctgatc
     2161 aacggtgaat gccaggtagc ccgcgcccag caattgacca gctcccggca cgcgatccgc
     2221 ttccagcaat gcgtccagct tctcctgctt ggtccgcgcg tagccgcgca gcgcgccggt
     2281 atcggagcaa tctgccagca gcatcgtcac cggcccgtcg cccttggcct gtaggctgaa
     2341 cgagccgcgg aatttcaggg ctgtggacag ggcagccgcc agcgccagcg actggccggc
     2401 gaggcgcgtc accgcgggat ggttcgcacg tcggttcaac agcgcgtcag ccagcgggcc
     2461 gagccgcacc agccggccac gtaccggccg cttcggcagg tagaaaggcg tgacgccgcg
     2521 cggcacaacg atatcgggca ccggcggacg gtcgccgtcg aggaaggctg gcgtttcgga
     2581 catcgcccca acataggcac ggccggtcgc gcgggctacc gtccgggtta atatgaattt
     2641 ccatcttcgt cccatcgtcc tggctgacgc gacagaagtc gccgcgctga tccggactgc
     2701 cttcgctgcg cagtccagcc tcacggaccc actgccctcg gcgatgcgcg tcaccgaggc
     2761 cgatgtctcg gcacatcttc gcaccggcgg cggcgccgtg gccgaggcca atggagcgat
     2821 cgtcggctcc gccctgtgga ccgagcagga tggcggcctc tatttgtccc gcctcgccgt
     2881 ggcgccggcc tggcgcggcc gtggtatcgc caaggcgctg gtcgcgtcgg gtgaggcggc
     2941 ggcgcgcgag aagaaactgc cgcgcatcca tctcagcacc cgcatcccgc tgctcgacaa
     3001 ccgccggctg ttcgcggcgt gcggcttcgt cgagacaacg cgtgaggcgc accccggcta
     3061 tgccgaaccg acctcggtgc agatggaaaa gcggctgcta gattagcagc gcagttggag
     3121 acgaaagatg ggcagccgcc cttggctgat tcttgccgcg gtggcgctcg cccgcatcgg
     3181 ctttggctac cagtaccaga ccgtggcctc gcttggcccc gagctgatgc accttttcca
     3241 gttcgactac gcgacgctgg gcacgctgat cggcgcattc atgttcttcg gcgccttcct
     3301 ggcgctgccc ctcgggctgc tgggccggcg cttcggcgat cggctcgtgc ttggtgccgg
     3361 cctcctgctg atggtggtgg ggccgctgat cagcacgttc tccgatggac ctgccggcat
     3421 cggcctaggt cgcggcctcg ctggcatcgg cgcggtcgca atgatcgtgc tgcagaacaa
     3481 gatcatcgcc gactggttca ccggccacca gctgatgtgg gcgctcagcg tgtcgtccgc
     3541 cgcctatccc gtaggcgtcg gcctggcgca gctcgtgttg ccgccgctga cccattccta
     3601 tggctggcag gccggcttcc tgtcggacag cgtaccgatg gcggtgtcgc tcgtgttgtt
     3661 cctggtcagc tatcgggctt ccccgcatgc tgtcgcccag ccgcgcactt tctcgctgcc
     3721 gagcgcgcgt gagtgcctgc tgctggcgat cgctggcctg atctggaccg cctataccgc
     3781 cggctactcc tcttacctga actacgtccc ctcattgatg gcggtgcgcg gagagggcct
     3841 ggtgttgacc ggcgtcgtgc tgacgatcgc gacctggggg aatgtgcccg ccaccatgtt
     3901 gggcgccggc cttgccacgc gcctgggcgc ctttcgcatc ttcctgttca gcacctgcgc
     3961 gcttgttgtc ggcatcggtg gggcagccgt gctcgactgg ccggtgacat tcgcggtgat
     4021 cattggcatc gttggctcgc tgcatcccgg cgtgatcatg gccgtcggca cgctgtcggc
     4081 gcggccggag aaccgcgccg tcggcatggg gctgttctat tcgatgtact acgcgggcgg
     4141 tgccgtcgtg ccggcgctgt gcggtcgcgc cgccgatatc tatggcagcc ccgagggcgc
     4201 cttgctcgcc gcatccgtca tttctgtact ggcgctcccg atgttcctgc tgcatcgccg
     4261 gatggcggcg cacgaaacga tgctggcgcg ggcataggcc ctgcaattcc aatcggccgc
     4321 cgcgcggcat agccgacgcg aatgccgggc tcgaacccca gggactgata gaacaggtgc
     4381 acgcgagggt cctgcctgcc actttgcagc agcacatggt ggcaatcgcg cgcccacgcc
     4441 tccgcgagtg cggcttgcac cacggcacgg ccgtggccct gaccgcgatg gtccggatgc
     4501 gtcacgacgt tctcgagaaa cccatgtctg cgcccgccgc gcagaagatt cggcgcggtg
     4561 atcagcaggc acgtcgcgac cacgaccgtg ttcgcgtctg acacgaacag cgccacatcg
     4621 tcgcgggcca acgtttcaga gaggatgcgc tcggcgcgct cgagcggttc ggcggccgca
     4681 ctcacttcgg agacgcgaaa cagttccagc aaaccatgca gatcggatgc tcgggccagg
     4741 cgtgctgtgc tcatggcagg ctccagttgt gaccccccgt catcgccggc ttgtgacccc
     4801 tcgtcatagc cggccttgag ccggccatca cactcggcag gcacgatggt gcctgggcag
     4861 gccgacgacc ggctcaatgt caggcgagag gttggggcgt agtcaacgag gcttgctact
     4921 tggtcttact ggaacgggat cgtggcgcaa cacggatcag tcgtcgtagc cgttcgaaac
     4981 aagcaggaag gatggtaaga ccgcgcattc gggcattctc aactcgatag ccaacagtgc
     5041 cgcctgctcg ggggcgagct ctgtcagcag gtcgtgtgct gcagcgaccc actgcatctc
     5101 ccccgtcaac tgatgcagcc tcagcagggc gaacgcgcgg gccaacggtt gacgtggccg
     5161 aggcgcatcc agcgcgcgct gcctgtaatc ctcgcgggcg aacaaatcat aggccgctgc
     5221 ccactcccat acactcgcct catcagcatc gattgcatcg agccgttccg cgagaccgtc
     5281 ctgcggcgaa actgctccag cctttgcaag cgtcagggcc gcgtgcatca ggtacgcgtc
     5341 gtcgcctgcc gcccgttgct gcggctccgc aaaaagccgg tgcagatgct gcgaagccca
     5401 tcgcccgagt tcagcggctg tgtcatcacg caaagcggtg ccttccagtg cctcagccga
     5461 ggccagtatc gccgcaatgg tgtctcgcct gtccgtcggc gggccggaat ccaggaaacg
     5521 ccggaagcat tgccgcacca accgggtatc cgaccgcgcg tgcgcgatcc gggtggcaac
     5581 cgactgaatg atccagtccg cgcccgcgtc ctcaatcagc accgccgctg cggcgaggag
     5641 ttcagcatcc tccaaagcca tggcagcgcg cagtgcgaac cacgcacggt gcaacggcgg
     5701 cagtatcgaa tgcgcgaggc gacgtgtctg ctcaattgcc gtttccagtt ctggcgccga
     5761 acgaggtgga ccactggtcg ccgctaaagc cgtggcggcg aagcttcgcg caagtccgct
     5821 catgttctcg aagcgatccg ccggctgtct cgccataccc cgtcgcagca ctcgctccac
     5881 ggcaggccac gccgcgatcc cccgggtcgc aaacggcaac ggcgggcgcg cagcaattcg
     5941 ctggagcaaa tcctcctgga ccgcgggtgc atcgatgggg tggcgcccgg tcagcagcag
     6001 ataggccagg acgcacaccg aatactgttc cgatagctcc gtacaggctg gcggcaatct
     6061 tcccgccagc aacgcctctg ccatcgccgg atcgtagaat tgcgggatgc cggcgcgggc
     6121 gggatcaatc tcgtcgccgg cgtcgatcag gctggcgcgg ccgaaatcaa tgagtgtcac
     6181 ggtgccgttg tcggcgacca ggcaattccc cgggtggatg tcgccatgca ggacgccgct
     6241 cgcgtgcagg ctactgtacg cctcgaacat cgcgcggacg aggcgatgca gtctgctacg
     6301 gtcgcgcgcc gcccgcgcct gttgcgctgc gatggcgacc gagacacctt cgcaccattc
     6361 catggcgata aagctgcgtc cctgctcaat cccatgggcc aagagcttcg gcgtgacggc
     6421 gccgttcagg cgctgcagaa tcgttgcttc gtggccaagg agcgccgcca ccgctgccgt
     6481 cttttcgcgc gcgatcttca gcgcgacaac agtaccgtcc tgactcctcg cccgataaac
     6541 ctcggtgtcg tccagcgacc acatcaccgc atcgatttcg aacgtgccaa acgcctggcc
     6601 cgccgcaaga cccggctccg atgcgcgtgc atcgatggca tcctccggaa cgaggatacg
     6661 cccatcgatc agccgcgcga tggcatcgaa tgccacatcg agcacagcgg acggatcctg
     6721 ttcgcgcgtg cggctgaatt gcagcaccgc gtcggtgatc aggcgcggtt cgcggaattc
     6781 ggcgatcaac gctgcgagcg tcgcggtgat gagtttcgcc gtcacccggg cgccgggccg
     6841 agttatgacc gactggtcat cctccaccgg tccgatccgc gcccgcaggc gagccgaaag
     6901 ttccaccacc tgcaggcatt tcgcatcagg aggcagtaca aaccgcgtct ccgtcaggtc
     6961 gggattcgcg gctgtcgtca tgtcgcgtcg aaacgcgccg atgcattatc gaccagcgac
     7021 catgagcacg gatccactcc gtctgctcga atgcctgcga gcgctgcgcc caccctgtcc
     7081 tcgacattca ttgctggcga tgcagaaaga gccgatgcca gcttttccgt tacccaggct
     7141 cgccagctcg ttggtgcatt tcccgccggg ggatcgacgc cggcggagac gatcccatcg
     7201 ccgccgacct cgctggtgaa cg
//
LOCUS       DATGEM010000342         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1469716, whole genome shotgun sequence.
ACCESSION   DATGEM010000342 DATGEM010000000
VERSION     DATGEM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469716"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VKQ27_10930"
     CDS             complement(<1..818)
                     /locus_tag="VKQ27_10930"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLY89485.1"
                     /translation="MLKPRLAALCLAAALVTIGGGNAFAQSDDDSMGGDTSLNLPNDV
                     AVFGKFDPAVRKATAIVNGFIITDTDVDQRLNLVLAANGQQVSADERDKLRLQVLRNL
                     IDETLEIQEAKSNSVEIPAGDIDQTFQRVAGQFKNTPDQFTTFLKSKGSSPNSIKRQI
                     EGELAWQRLLDRKVQPFINVGEDEVKQVIARLNASKGAKEYHVGEIFLSATPQTDEQV
                     HANADRVMDQIRNGGSFVAYARQYSEASTAAVGGDLGWVRAEQLPDQISQLLPQ"
     gene            complement(946..3282)
                     /gene="lptD"
                     /locus_tag="VKQ27_10935"
     CDS             complement(946..3282)
                     /gene="lptD"
                     /locus_tag="VKQ27_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HLY89486.1"
                     /translation="MPAAAQTTSPAASDPSAKVGSADAASATGGISVAGGAFGAQPDG
                     RPALVPSNASATFPAAGANAPLSDDEIVFTADTLDYDYKEHVVVASGDVRMLRQGNRL
                     RADRVEWNRDTGEVHAMGNVAIANPQGDITYGRDLQLTDTLKDGVIEDLLLVLENGGR
                     MAATTGGRANGIYTLTHAAYTPCDVTGSKGCPKDPIWKITAARVVYDPNRHRLSYRDA
                     RLTFLGVPILWLPAFSHPDGSGQSGTSGLLVPDISYNGRNGFEVATPYYIRLAPNRDL
                     TVTPYIYTNVLPMLEAEYRSLDSHGAWRVQAYATDSARAVNDITPGSRRFRGYLEANG
                     TYQLDPYWRVSASIRAVTDKTFLRRYDINDDDRLRSTIKAERIDADSYLSIAGWEFET
                     MVPGVRQKTQPIALPVLDYRLRAPEHLLGGTFTFEANTLAITRIEGQDTQRAFVSAQW
                     EKWLLTPFGQQVTFTAYGRGDIYHSSDNLATSTVIYQGMPGWEGRGIGAVAADVRWPF
                     VGDFLTGTQRITPRVQIVASPKTRNLAIPNEDARAIDLEDSNLFALNRFPGYDRWEDG
                     ARVTYGLEYALDLPHFSLRSVVGQSYRFYTPSDIASGATGFGILPPGTGLSGRTSDIV
                     GRTTLRYGSFLSLTHRFRVDRKSFAVRRNEIDATLGNQQTYFEAGYLKLNRHISDTIE
                     DLRDHEELRLGGRLALLKRWSLFGSTTLDLSGDKNNPLVNPNINENGDTVKSRVGLAY
                     EDDCFEFSVSWRRDYTSIGDARRGNTIQFRLAFKNLGR"
BASE COUNT          604 a         1095 c         1208 g          674 t
ORIGIN      
        1 atctgcggca ggagctggga aatctgatcg ggtagctgct cggcgcggac ccagccgaga
       61 tcgccgccca ccgccgcggt cgaggcttcg gaatattgac gggcataagc gacgaagctg
      121 ccgccgttgc ggatctggtc catcacgcga tcggcgttgg cgtgaacctg ctcatcggtt
      181 tgcggagttg ccgaaaggaa gatttcgccg acatgatatt ccttggcgcc cttcgaggcg
      241 ttcaggcgtg cgatcacctg cttcacctcg tcctcgccga cgttgatgaa cggctgcacc
      301 ttgcgatcga gcaggcgctg ccacgcgagc tcaccctcga tctgccgctt gatcgagttg
      361 ggcgaagagc ccttcgattt caggaaggtc gtgaactggt caggcgtgtt tttgaactgc
      421 ccggcgacgc gctggaacgt ctggtcgatg tcccccgccg ggatttccac gctgttggac
      481 ttggcttcct gaatttccag cgtctcgtcg atgaggttgc gcagcacctg gaggcgcaac
      541 ttgtcgcgct cgtccgcgct gacctgctgg ccattggcgg ccagcacgag gttcaggcgc
      601 tggtcgacgt cggtatcggt gatgatgaag ccgttgacga tcgcggtcgc cttgcggacc
      661 gccggatcga acttgccgaa gacagcgacg tcgttcggaa ggttgagcga cgtatcgccg
      721 cccatagagt cgtcatcgct ctgcgcgaag gcgtttcctc caccgatcgt caccagcgct
      781 gcggccaggc aaagcgccgc caggcgcggc ttgagcatcc gtttattgga aatcacgctg
      841 tcctcagtgt cccgcccgtc gcgtcgcgcc ctgccgcagc tccccccgtg agagcgtcgt
      901 tgcggcaacc tgcaggcatg tcgcatgtcc tcgatgaata cagacttagc gtccgaggtt
      961 cttgaacgcc agccggaact ggatggtgtt tccgcgccgg gcgtcgccga tcgacgtata
     1021 gtcgcgccgc cagcttacgc tgaattcgaa gcaatcatcc tcgtaggcga ggccgacgcg
     1081 cgatttcacc gtatcgccat tttcgttgat gttcggattg accagtggat tgttcttgtc
     1141 gccggagaga tcgagcgtgg tcgagccaaa cagcgaccag cgcttgagga gcgccaggcg
     1201 accaccgagg cggagttcct catggtcccg cagatcctcg atcgtgtcgc tgatatggcg
     1261 gttgagcttg agatagcccg cctcgaaata ggtctgctga ttgccgagcg tcgcgtcgat
     1321 ctcgttgcgg cggacggcga aactcttccg gtcgacacgg aaacgatggg tgaggctcag
     1381 gaagcttccg tagcgcagcg tggtgcgccc gacgatgtcg gaggtccgtc ccgaaaggcc
     1441 cgtccccggc ggaaggatgc cgaacccggt ggcacccgac gcaatgtccg acggggtgta
     1501 gaagcgatag ctctggccga cgaccgatcg cagcgagaag tgcggcagat ccagcgcata
     1561 ttcgaggccg taggtgacgc gcgcgccatc ttcccagcga tcatagcccg gaaagcggtt
     1621 gagcgcgaaa aggttcgaat cctccagatc gatcgcgcgt gcatcctcgt tggggatggc
     1681 gaggttgcgc gtcttgggtg aggcaacgat ctggacacgc ggcgtgatgc gctgtgtgcc
     1741 ggtcaggaaa tcgccgacga acggccagcg gacatcggcc gcgaccgccc cgatcccacg
     1801 cccctcccag ccgggcatgc cctggtagat gacggtcgac gtcgcgagat tgtcgctgct
     1861 gtggtagatg tcgccacggc cataagcggt aaaagtcact tgctgaccga atggtgtgag
     1921 cagccacttc tcccattgtg cgctgacgaa cgcgcgctgg gtgtcctggc cctcgatccg
     1981 ggtgatcgcg agcgtgttgg cctcgaaggt gaaggtgccg ccgagcagat gctcgggtgc
     2041 gcgcagccga tagtcgagta ccggaagcgc gatcggctgg gtcttctgcc ttacgccggg
     2101 aaccatcgtc tcgaattccc agccagcgat cgagagatag ctgtccgcat cgatccgctc
     2161 ggctttgatc gtcgagcgca gccggtcatc gtcgttgatg tcgtagcggc gcaggaaggt
     2221 cttgtcggtg acggcgcgga tcgaggcgct cacccgccag tagggatcga gctgatatgt
     2281 cccgttcgcc tcgagatagc cgcggaagcg gcgcgagccg ggcgtgatat cgttgacggc
     2341 acgggcgctg tccgtggcgt aggcctgcac gcgccacgcg ccatggctgt cgagcgaacg
     2401 gtactcggcc tccagcatcg gcagcacgtt ggtgtagata tagggcgtga ccgtcaggtc
     2461 gcgattgggc gcgagccgga tgtaataggg cgtcgcgacc tcgaagccgt tgcggccgtt
     2521 gtagctgatg tccgggacca gcaggcccga cgtgccgctc tggcccgacc cgtccggatg
     2581 cgagaaagcg ggaagccaca ggatcggcac gccgaggaag gtgagccgtg cgtcgcgata
     2641 ggacaggcga tggcggttcg gatcatagac cacgcgtgcc gcagtgatct tccagatcgg
     2701 atccttgggg cagcccttgc tgccggtgac gtcgcagggc gtataggcgg catgggtgag
     2761 cgtatagatg ccgttcgccc ggccgccggt ggtggcggcc atgcgcccgc cattctctag
     2821 caccagcagc agatcctcga tcacgccgtc cttcagcgtg tcggtgagct gcaggtcgcg
     2881 cccataggta atgtcgccct gcgggttggc gatcgcgaca ttgcccattg catggacctc
     2941 gccggtgtcg cggttccact cgacccgatc ggcgcggaga cggttgccct gtcgcaacat
     3001 ccgcacgtcg cccgacgcga cgacgacatg ctccttgtaa tcatagtcca gcgtgtcggc
     3061 cgtgaagacg atttcgtcgt cggagagcgg ggcgttcgct ccggcggcgg ggaaggtcgc
     3121 ggaagcgttg gagggcacca gtgcggggcg accatccggc tgcgcgccga acgcgcctcc
     3181 cgcgacactg atgcctccgg tcgcgctggc cgcatcagcc gaaccgacct tcgcgctcgg
     3241 atcgctggct gcgggggatg tcgtctgagc ggccgcgggc attgcggcca cgaccaacac
     3301 cagcggcagc gcagtagcga gccttaggcg aaaagatgat cgtgtcgggc tgatgaagtt
     3361 cacttttccc ccagagtgcc cgaacggcat cgctttcctg cgcctatcgc accgccgggc
     3421 cggctccgca atgtctcgcg gcgctcgggt gcgatggctc gtagggctgg cgcggaccgg
     3481 cgagggttgc catatgcgcg accaaggcgc ttggtggcgc cccgtaacgg aggtttcaaa
     3541 gttccgatga acgtgcgctt cgcagatcgt cgccccgaag g
//
LOCUS       DATGEM010000343         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1696986, whole genome shotgun sequence.
ACCESSION   DATGEM010000343 DATGEM010000000
VERSION     DATGEM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696986"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VKQ27_10940"
     CDS             <1..327
                     /locus_tag="VKQ27_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89487.1"
                     /translation="YAPLIAQAREADASFRRTLDEERGALTRGRSAAMGSEAWTAAQQ
                     ALSRVETARGPVVRTLSELDAARDAEITHTDSGEAIAAANAFEQVRRIDEAEAGALTQ
                     AWPPAP"
     gene            complement(735..914)
                     /locus_tag="VKQ27_10945"
     CDS             complement(735..914)
                     /locus_tag="VKQ27_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89488.1"
                     /translation="MISATKRYRVAPFGGDAILVSAADCGPLGRLLDHDGLQGWRGAL
                     TGRVRGATLAPGTWN"
     gene            complement(1018..2952)
                     /locus_tag="VKQ27_10950"
     CDS             complement(1018..2952)
                     /locus_tag="VKQ27_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HLY89489.1"
                     /translation="MPLDPRYPADRLSFMLADSAASRLLIDATQARLSFEGRVANMED
                     EAALTQSCPAVPPPPQGDGRDLAYIIYTSGSTGVPKGVMLAHTVLGLIDWFERVLTPE
                     ALARVAATSSICFDPSVVEIFLPLCTGGCVVLKENLLDPFAPEEAPTLLQGVASALRE
                     IARAGVIPPSVRVLHVGGEPLSADVARELYRTTSVEDIFNHYGPTEATTAATVALVER
                     DAQDRPTIGYPVAGACIHLLTPEGKPIGDGDVGEIYIGGSGVACGYWNRPELDGERFL
                     PDPFSGADGARMYRTGDLARRLPDGALAFLGRVEGQVKILGHRVELAEIELTLQRIAG
                     VDDEAVLADDAPNGQTKLIAFVSGSSASDMAAVRGSLAKWLPRAMRPSKIVRVESFPR
                     TLSGKIDYKALPGLIPRRSGKDALPARTETGIEERGLEATITRIFRELLDQSDVGPHD
                     DFFDLGGDSLLAYRLALEIEAAIDRPVSPVVVGQASTPRALAELIDAITEIANEHICM
                     LAKGGPDRPVFCLPDVDGQSSNFVTFAKLLEKHRPVYGLIPIAGRAQRQPGDRAAFHR
                     ELSRSPSRSAAAGALLYRRIFLRRRRRVRSGAQSGSRRRGGHADPHRRAGGAPLFRVA
                     GPASLALAAWRFLPQADGAT"
     gene            complement(2877..3332)
                     /locus_tag="VKQ27_10955"
     CDS             complement(2877..3332)
                     /locus_tag="VKQ27_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY89490.1"
                     /translation="MTMDFSVERSLAADPSTAGLQARSETHPADATNANDAHQPGVGG
                     QIWAVDRIDAAVRRQAMRSPEVQAVVWKGTALTYGELEVRSNRLAHYLRRLGVGPDVR
                     VAVMLQRTPSLIVALLAILKAGGAMCRSIPATLPIACPSCWPTARRAGS"
     gene            complement(3329..3997)
                     /locus_tag="VKQ27_10960"
     CDS             complement(3329..3997)
                     /locus_tag="VKQ27_10960"
                     /inference="COORDINATES: protein motif:HMM:NF044569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89491.1"
                     /translation="MPVACEERYSRDSRARLLTPGEVIGGHARYWQYDDASRAGLIRI
                     AHFVTGTLAKPHPDLGRAGAVCPYVLLSLANHRLLLSTSPLETDDLDQIMEEMITIGS
                     MFTHMIGSEIINKELRISIIHIFLKVSFNNRFESIKVVQKTLEQRFVDRGLVIGEFYP
                     GCEAYGLHSPTFRPFDAPVAALAVREMTLAHAPCLVSRESFRRAYLDQFGDAGRRQIA
                     GLTQ"
     gene            complement(4363..4581)
                     /locus_tag="VKQ27_10965"
     CDS             complement(4363..4581)
                     /locus_tag="VKQ27_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89492.1"
                     /translation="MADLPDGFDRLKAELLWLHDLGLLPAQTMPSPSWTGCQRFADAL
                     AEHLESPHFLTFLSLAMLLWTGVYASWR"
     gene            4739..>4957
                     /locus_tag="VKQ27_10970"
     CDS             4739..>4957
                     /locus_tag="VKQ27_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89493.1"
                     /translation="MSSMRYSDFDLHHPSFLAPRSIPLLAGRWLVVGIAGGVVMDILL
                     LLSAHLRIDISRASLGFAAWAVASGVICY"
BASE COUNT          977 a         1607 c         1533 g          840 t
ORIGIN      
        1 tacgcccccc ttatcgccca ggcgcgcgag gccgacgcct cgttccgccg cacgctcgac
       61 gaggagcgcg gcgcgctgac ccgcggacgt tcggccgcga tgggcagcga agcctggacg
      121 gccgcgcagc aggcgctgtc acgggtggag acggcgcgcg ggccggtggt gcgcaccttg
      181 agcgaactcg atgccgcgcg tgatgccgag atcactcata ccgatagcgg tgaggcgatc
      241 gccgctgcca atgcgttcga gcaggtccgc cggatcgacg aagcggaagc gggcgctctg
      301 acccaagcgt ggccccccgc cccctgaccc tgcgcgacgg tggacaaatc gggacagcgc
      361 gtcgcaattg ccccattcgc acgccgacga gccgcacatg tcgagtccgc aattcccgct
      421 gaggcccgtc catcgtatgc tgcagcgcag ctacgaagga gtacggccct atggcacgtc
      481 tttttggaag cctgcaccgg ttgacacgga tcgcattatg gcggcgcaca agagccgcga
      541 ccatctctac aaccttctgg ctcggcgcga acgcacccga acttagcagc gaatagcgcg
      601 cgccctcccc atcgcccggg cacatcgcct cccggacggg tgccctgggc gcgaggctcc
      661 ttaggcggaa ttgtcgcggc cgcagagcca tatctgcaac agggcgacga gttggctcac
      721 ccaggggttg cgcatcaatt ccatgtgccc ggtgcaagcg tggcgccccg cacccgcccg
      781 gtcagcgccc cccgccagcc ttgcagaccg tcatggtcaa gcagccggcc aagggggccg
      841 cagtcggctg cgctcaccag gatcgcatcg ccgccgaagg gggccacgcg atagcgtttt
      901 gtcgcgctga tcatggcacg agccaacgtc ctgctgcgct cagagaccca gatcggcgcc
      961 tgatcctgcg aggagcccca tttcgtccag cgcgagccac ggaccgtctc gatcgcctca
     1021 tgtagccccg tctgcttgag gtaagaaacg ccatgcagcc aaagccagcg aagcaggacc
     1081 ggcaacccga aacaacggcg ccccaccagc gcgtcgatga ggatcagcgt gacctcctcg
     1141 ccttcggctt ccagactgcg cgccagatcg aacgcggcga cgccgccgaa ggaatatccg
     1201 gcgatacaat aaggcccctg cggctgctga gcgcgaaggg ctgcgagata gttcgcggtg
     1261 aaatgcggca cggtcgcccg gttggcgctg ggctctgccg gcaataggaa ttaggccgta
     1321 gaccgggcga tgcttctcca gcagcttcgc gaaggtcacg aagttgctcg attggccgtc
     1381 gacatccggc aagcagaaga ccggcctgtc cgggccgccc ttcgccagca tacagatatg
     1441 ttcgttcgcg atctcggtga tcgcgtcgat cagctcggcc agagcgcgtg gcgtgctggc
     1501 ttgccccacg acgacggggg acaccgggcg atcgatcgcg gcttcgattt ccaaagccag
     1561 acgataggcc agcaacgaat ccccgcccag atcgaagaaa tcgtcgtgag ggccgacgtc
     1621 ggactgatcc agcagctccc ggaagatgcg ggtgatggtc gcctcgaggc ctctctcctc
     1681 gatcccggtc tcggtgcgag cgggcaaggc atctttcccc gatcgtcgcg ggatcaggcc
     1741 gggaagcgcc ttatagtcga tcttgcccga caatgttctg gggaagctct ccacccggac
     1801 gatcttcgaa ggccgcatcg cgcgcgggag ccatttcgcc agggatccgc gcacggccgc
     1861 catgtcggaa gccgacgacc ccgatacgaa ggcgatcaac ttggtttgcc cgttcggcgc
     1921 atcgtcggcg agcacggcct catcgtccac gccggcgatg cgctggaggg tcagttcgat
     1981 ctccgccagc tcgacacgat ggccgaggat cttcacctgg ccctcgacgc ggcccaggaa
     2041 cgccagcgca ccatcgggca gccggcgggc aaggtctccg gtacgataca ttcgggcacc
     2101 atcggcgcca ctgaacggat cgggcaggaa gcgttcgccg tcgagctccg gtcgattcca
     2161 ataaccgcat gcgacgccgg aaccgccgat atagatctca ccgacatcgc cgtcgccgat
     2221 cggcttcccc tccggcgtga gaaggtggat gcaggcccct gccacgggat atccgatcgt
     2281 cggcctgtcc tgagcatccc gttcgaccag cgccaccgtc gccgcggtgg tcgcttcggt
     2341 cgggccataa tggttgaaga tatcctcgac gctcgtcgtc cgatacagtt cgcgcgcgac
     2401 atcggctgac agcggctcgc ccccgacgtg gaggacgcgg acgcttggcg gaatgacccc
     2461 ggcacgtgca atctcgcgca acgcagatgc cacgccctga agaagcgtag gcgcttcttc
     2521 aggggcgaag ggatcgagca ggttctcctt gagcaccacg catccccccg tacagagcgg
     2581 gagaaagatc tcgacgaccg acggatcgaa acagatcgag ctggtggcgg ccaccctggc
     2641 caaggcttcg ggcgtcagca cccgctcgaa ccagtcgatc aagccgagga cggtgtgggc
     2701 cagcatcacc cccttgggca caccggtcga tcccgaagtg tagataatat aagcgagatc
     2761 cctgccatcg ccctgtggcg ggggcggcac ggcaggacag gactgcgtca gggcagcttc
     2821 gtcctccata ttcgcaaccc tgccctcgaa cgatagtcgg gcttgtgtag cgtcgatcag
     2881 gagccggctc gccgcgctgt cggccagcat gaaggacagg cgatcggcag ggtagcgggg
     2941 atcgagcggc acatagcccc cccggccttc aatatcgcca gcagggcgac gatcagcgag
     3001 ggtgtccgct gcagcatgac ggcaacgcgg acgtcggggc cgacgccaag gcgacgaaga
     3061 taatgcgcca gccggttgga ccggacctcc agctccccat aagtgagcgc cgttcccttc
     3121 cagacgacgg cctgcacctc gggcgatcgc atcgcctgcc ggcgaacggc cgcatcgatc
     3181 cggtcgacgg cccagatctg gccgccaacg cccggctgat gtgcgtcatt agcgttggtg
     3241 gcgtccgctg gatgggtctc cgaccttgcc tggaggccgg ccgtgctcgg atcggcggcg
     3301 agcgaacgct cgacagaaaa atccatggtc attgtgtcag ccccgcgatc tgtcgccggc
     3361 ctgcatcacc gaactgatcg agataggccc tgcgaaagct ctcgcgggaa acgagacatg
     3421 gtgcgtgcgc cagcgtcatt tcgcgcaccg ccagagcggc aaccggcgcg tcgaatggac
     3481 gaaatgtcgg actatggaga ccatacgctt cacatcccgg gtaaaactcc ccgatcacaa
     3541 gccctcgatc gacaaatctt tgctcaagtg tcttctgaac tactttgata gactcaaaac
     3601 ggttattgaa tgacactttt agaaatatat gaataataga tattcgcaat tctttattta
     3661 taatttcact gccaatcata tgtgtgaaca tcgatccgat cgtgatcatt tcctccatga
     3721 tctgatcaag atcatcggtt tcaagcggtg aggtcgacag caacaagcgg tggtttgcga
     3781 gtgatagcag cacgtaaggg cacaccgcgc cggcccggcc gagatcggga tgaggcttgg
     3841 ccagcgtgcc ggttacgaag tgcgcgatgc ggatgaggcc tgctcgcgag gcatcgtcat
     3901 attgccagta gcgtgcatgg ccaccgatca cctcgccggg tgtcaggaga cgcgcgcgac
     3961 tatcgcgact ataacgctcc tcacaagcca caggcactgg cccccccctg actggccatc
     4021 tccctcaaca gtaaggatta tgctcgcgag aaaagagccg gcaaacgaag caaaggggga
     4081 gcgcctggtg aattggggcg cggcggatgg gatccgcagg gcgactcccg cgcgaagctg
     4141 ccatggtcga cgccgagccg cgatatccag cgcccggcag cggatcgatc tcgaatattt
     4201 tatcttccag gatgtcgaga gcgatggcgt acacaatcgc ccacccgtca gcaccgccat
     4261 aggagcaatc acgatcgttg tcataaacac agacgcatca cgaagcgatg agatcgccat
     4321 cgcccgacga gggactggat cggctgttca ttaatgccgt tgtcagcgcc atgacgcgta
     4381 cacgcccgtc caaagcagca tggcaagaga aagaaacgta agaaagtgtg gagattcaag
     4441 atgctcggcg agagcatcgg cgaaccgctg gcaccctgtc cagcttggtg acggcatggt
     4501 ctgagccggc aacaagccga gatcgtgaag ccacagaagc tcagccttga gcctgtcaaa
     4561 accatccggc agatcggcca cagcgcatcc tcgatgggag gaagttcgcc ccctcctgcc
     4621 tctagccggc tattgagccg ggcaccactg tcacaaaata gtcactgaca cggcgtgcta
     4681 tgcctggtgc gagatcatcc gatgacacca ttggcccatg tcgtgctagg cgccgccgat
     4741 gagcagcatg cgatattccg atttcgactt gcatcacccg tccttcctgg cgcctcgctc
     4801 cattccgctt cttgcgggcc ggtggctggt cgtgggaatc gcggggggcg tcgtgatgga
     4861 tatcctcctc ctgctgtcgg cgcacctgcg catcgacatc tcgcgggcca gccttggctt
     4921 cgcggcatgg gccgtcgcct ccggagtgat ctgttat
//
LOCUS       DATGEM010000344         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1433503, whole genome shotgun sequence.
ACCESSION   DATGEM010000344 DATGEM010000000
VERSION     DATGEM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433503"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VKQ27_10975"
     CDS             complement(<1..1222)
                     /locus_tag="VKQ27_10975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLY89494.1"
                     /translation="MAEDLPPREAMEFDVVIVGGGPSGLAAAIRLKQLIPDAAVCLVE
                     KGSEVGAHILSGACLEPRALNELIPDWKEKGAPLDTPATDDRFLFLTATRSFRMPTPP
                     QMHNHGNYIVSLGNFCRWLATQAEELGVEIYPGFAAAELLEEDGRVVGIATGDMGIGK
                     DGQPTDNFQRGMELRARYTLLAEGCRGSLSKQIIRKFSLRDHCDPQTYAIGIKELWEI
                     PAENHKAGLVEHTIGWPLDSSTYGGSFVYHFGNNLVSFGFVIGLDYRNPWLSPFEEMQ
                     RFKTHPEMRKHFEGGRRISYGARALNEGGLQSIPRLIFPGGALIGDAAGFVNVPKIKG
                     THTSMKSGMVAAETVAEALAAGDARDLSAYEARLRECWVWDELSRVRNVRPSFAKFGL
                     WGGLAYSAIDTYLLR"
     gene            1329..2843
                     /locus_tag="VKQ27_10980"
     CDS             1329..2843
                     /locus_tag="VKQ27_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY89495.1"
                     /translation="MSGPAKTDHEIDELRRRLAALEAQATGLGKAQVMTRDFAGEIRF
                     WAGGMERLYGYTAAEAVGRNSHNLLCTEFPQSLRDLERELLESEHWTGELHHRRRDGE
                     EIVVVSHQSLLRGDTPLVTEANNDITEAWRGREARLYLASIVESSEDAIIGKTLSGII
                     TSWNRAAEAIFGYTSPEVIGQSITLLHPPDRLHEEAMILERLSRGERISHFETTRLRK
                     DGSEIAVTLTMSPIYDHSGQIIGVSKIVRDITPERRSQSRIAGLQAELVHVARLSTMG
                     QMASAIAHELNQPLTAVSNYVGALGRMLAAGSVDAERAQDIVNRIRAQASRAGDVIRR
                     LRDHVAKRAAVRKPENLNAVIGEAVELGLVGTRQHNMQSDLRLDPAVPEVSIDRIQIG
                     QVLINLVRNAAEAMQAGERRELSVVTRLNGDMAEIVVSDTGPGIAPEVADRLFQSFVT
                     TKPSGLGLGLSICREIVEAHGGQIAASTNAAGGATFTVTLPLSRGEEDHDPAAR"
     gene            complement(2803..3309)
                     /locus_tag="VKQ27_10985"
     CDS             complement(2803..3309)
                     /locus_tag="VKQ27_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HLY89496.1"
                     /translation="MISLRLAKIAMAASMAAFAGLVAIDNVIDYGTNFSFVEHVLSMD
                     TTFTTNTLRWRAITEPVLWHAAYWVIIIGEALTGAAYTIGAFMLTRALRAGGEDFNNS
                     KHFVHLATALGFLVWFFGFMVVGGEWFSMWQSPQWNGQEAAFRFYVAVLGVGIFIALQ
                     DRDLPRRG"
     gene            complement(3349..>3477)
                     /locus_tag="VKQ27_10990"
     CDS             complement(3349..>3477)
                     /locus_tag="VKQ27_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLY89497.1"
                     /translation="KYMGMERSTFLVDKTGKIAKAWRKVSVTGHAAAVLAAAQALK"
BASE COUNT          672 a         1138 c         1082 g          585 t
ORIGIN      
        1 cgcgcagcag gtaagtatca atcgcggaat aggcgaggcc gccccatagt ccaaacttcg
       61 cgaacgacgg acgaacgttc cttacccgcg acaactcgtc ccaaacccaa cactcgcgca
      121 acctcgcttc gtaggccgac aggtcccggg catcgcccgc cgctagtgcc tcggccacgg
      181 tttcggccgc gaccatgccg gacttcatcg acgtatgcgt gcccttgatc ttcgggacgt
      241 tcacgaagcc ggcggcgtcg ccgatcagcg caccgcccgg gaaaatcagg cgggggatcg
      301 attgcagccc gccttcattc agcgcgcgcg ctccatagct gatgcggcgg ccaccttcga
      361 aatgcttgcg catctccgga tgcgtcttga accgctgcat ctcctcaaac ggtgaaagcc
      421 agggattgcg atagtcgagg ccgatgacga aaccaaagga aaccaaattg tttccgaaat
      481 gatacacgaa cgaaccgccg taggtgctgc tgtcgagcgg ccagccgatc gtgtgctcga
      541 ccaggccggc cttatggttc tcggcgggga tttcccacag ttccttgatg ccgatggcgt
      601 aagtctgcgg atcgcaatgg tcgcgcaggc tgaatttgcg gatgatctgc ttggacagcg
      661 agccgcggca gccctcggcg agcagcgtgt agcgcgcacg cagctccata ccgcgctgga
      721 aattgtcggt aggctggccg tccttgccga tgcccatatc gccggtggca atgccgacga
      781 cgcggccgtc ttcctccagc agctcggccg cggcgaagcc ggggtagatc tcgaccccaa
      841 gctcctcggc ctgggtcgcc agccagcggc agaaattgcc gagtgagacg atgtagttgc
      901 cgtggttgtg catctgcggc ggcgtcggca tgcgaaagga gcgcgtcgcc gtcaggaaca
      961 ggaaccgatc gtcggtcgcc ggcgtgtcga gcggggcacc cttctccttc cagtccggga
     1021 tcagctcgtt caaggcgcgc ggttcgaggc aggcgccgga caggatgtgc gcgccaactt
     1081 cgcttccctt ttccaccagg cagaccgcgg catccggaat cagttgcttc aggcggatcg
     1141 cggcggcgag accggatggg cccccgccca cgataaccac gtcgaactcc attgcctccc
     1201 gcgggggcaa gtcctcggcc atctcgaatc ctcccggact gtcgggcggc aacctaggca
     1261 gactggttgc gacacgcaaa gagacggcct ttaccacccg cccacatgaa tgtgatacgc
     1321 tccctcgcat gagcggaccc gccaagacgg atcacgaaat tgacgagctg cgccggcgcc
     1381 ttgcggcgct cgaggcgcag gcgaccggtt tgggcaaggc ccaggtcatg acgcgggatt
     1441 tcgccggcga gatccgcttc tgggcgggcg gcatggaacg gttgtacggc tacaccgctg
     1501 ccgaggcggt cggccgcaat tcccacaact tgctgtgtac cgaattcccc cagtcgttgc
     1561 gcgatctcga acgcgagttg ctcgaaagcg agcactggac cggcgaactg caccaccgca
     1621 ggcgagacgg cgaagagatc gtcgtcgtca gccaccagtc actgctgcgt ggcgacacgc
     1681 cgctcgtcac cgaggccaac aacgacatca ccgaggcttg gcgaggccgc gaggcacggc
     1741 tgtacctcgc cagcattgtc gaatcgtcgg aagacgccat catcggcaag acgctgtccg
     1801 gcatcatcac cagctggaac cgtgccgccg aggcgatctt cggttacacc agccccgaag
     1861 tgatcggcca gtccatcaca ttgctgcatc cgccggatcg gctgcacgaa gaggctatga
     1921 tcctggagcg gctgagccgc ggcgaacgga tcagccattt cgaaacgacg cgcctgcgaa
     1981 aggatggcag cgagatagcg gtgacgctga ccatgtcacc catctacgat cactcagggc
     2041 agatcatcgg ggtctccaag atcgtgcgcg acatcacccc cgagcggcga agccagtcgc
     2101 gcatcgcggg actccaggcc gagctggtcc atgtcgcgcg gttgtccacc atgggccaga
     2161 tggcgtcggc gattgcacac gagcttaacc agccactgac ggcggtctcc aattacgtcg
     2221 gcgcgcttgg acgcatgctg gcagcaggca gcgtcgacgc ggagcgggcg caggacatcg
     2281 tcaatcgtat cagagcgcag gccagccgcg ccggggatgt gatccgccgg ctacgcgacc
     2341 acgtcgcaaa gcgtgccgca gttcgcaagc cggagaacct gaacgcagtc atcggggaag
     2401 cggtggagct aggtctcgtc ggcacccggc aacacaatat gcagagcgac ctccgattgg
     2461 atccggctgt cccagaggtg tcaatcgatc gcatccagat tggtcaggtg ctgatcaacc
     2521 tggtgcgcaa cgccgccgaa gcgatgcaag ccggcgagcg acgcgaattg tccgtcgtga
     2581 cccgattgaa cggcgacatg gcggaaattg tggtcagcga caccggacca ggcatcgcgc
     2641 cggaagtggc cgaccgcctg ttccagtcgt tcgtcacgac caagccgtca ggccttgggc
     2701 ttggcctatc gatctgccgt gagatcgtcg aggcacacgg tggccagatc gccgcgtcca
     2761 ccaacgcggc tggcggtgcc accttcaccg ttacactgcc gctcagccgc ggcgaggaag
     2821 atcacgatcc tgcagcgcga tgaaaatgcc cacgcccagc accgcgacat agaagcggaa
     2881 tgctgcctcc tgcccgttcc actgcggcga ttgccacatc gagaaccact cgccgcccac
     2941 caccatgaag ccgaagaacc acaccaggaa cccgagcgcg gtcgcgagat ggacgaagtg
     3001 cttggagttg ttgaagtctt cgccgccggc acgtagtgcg cgggtcagca tgaaggcgcc
     3061 gatggtgtag gccgcaccgg tcagcgcctc gccaatgatg atgacccaat aggcggcgtg
     3121 ccacagcacc ggctcggtga tcgcgcgcca gcgcagcgtg ttggtggtga atgtcgtgtc
     3181 catgctcagc acgtgctcca cgaagctgaa attcgtgccg tagtctatga cgttgtcgat
     3241 ggcaaccagg ccggcgaatg ccgccatcga tgcggccatg gcgatcttgg ccaggcggag
     3301 ggagatcatg tcgctgctat ctcccctccc cctccttccc cagcctcgct atttcaatgc
     3361 ctgcgccgcc gccagcacag ccgcggcatg gccggtgacg ctgaccttgc gccaggcctt
     3421 ggcgattttc cccgtcttgt cgaccaggaa cgtgctgcgc tccatgccca tatactt
//
LOCUS       DATGEM010000345         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1062014, whole genome shotgun sequence.
ACCESSION   DATGEM010000345 DATGEM010000000
VERSION     DATGEM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062014"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1270
                     /locus_tag="VKQ27_10995"
     CDS             233..1270
                     /locus_tag="VKQ27_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY89498.1"
                     /translation="MSARAEQIRFGVIGINHGHIYGMANQLLDAGAELVAFFAPEDDL
                     AAEFSNKFPQARRARSKAEVLEDRSLALIASAGIPNERAPLGVETMRHGKDYLSDKPG
                     FTTLEQLEEARSVQRQTRRIYSVCYSERVQNRATVRAGELVEAGAIGRPLQTVGLGPH
                     RLNAPSRPKWFFEHDRYGGILTDIGSHQVDQFLFFTGSTSAEVVASQVGNVAHPEYPG
                     LDDFGDMLLRGDGGTGYVRVDWFTPDGLSAWGDGRLTILGTDGFIEIRKNVDIGGRAG
                     GDHLFIVDQRETRYMDCKNVELPFGPRLLDDVRNRTETAMPQAHTFLASELALNAQAQ
                     ARSITRQSVAA"
     gene            1267..2346
                     /locus_tag="VKQ27_11000"
     CDS             1267..2346
                     /locus_tag="VKQ27_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY89499.1"
                     /translation="MSTPDRLRVAVVGAGIGRSHIRGYQAVGDRFELLALCDIDAARG
                     KPVAEEHHIPRFVTDFAELLRMDDLDVIDICTPPHLHFEQIQQVLASGKHAICEKPLV
                     SSLREVDGLKRLEASSGRRIMPIFQYRFGHGMQKLKLLLERGIAGKPYLATVETAWRR
                     RPAYYDVPWRGKWATERGGALLGHAIHNHDLLCYVLGAPKRLWAYATTRVNAIEVEDC
                     AAIAMEMADGSAASSGVTLGSAEEITRHRFSFSGLVAESNTRPYTNSGDPWTFVGDSP
                     DRDEEIALTLARFKPLPEHYPGQFYRFYDAIRSGGELPVTLADARLSIELITGMYYSS
                     ETGQAVTFPIGADHPRYTSWIPAGK"
     gene            2468..>3383
                     /locus_tag="VKQ27_11005"
     CDS             2468..>3383
                     /locus_tag="VKQ27_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY89500.1"
                     /translation="MTVAALLAGCGTGSSNYTPAGGAAAPTQPAGAAAGQPTTAAAAA
                     GGQKTHLVYFNARGAEAAERALVNRYMQDHPNIEIEYLSTTSLNGPSDTDAIANLIFN
                     IQAKKTIDIAKIEVSRTPLDLMAASAIQELNAVGGDAVKNQLKGLLNNNYVDINNGTW
                     AIPYEFDPFGYVYNADMYKDAGLNPDNPPKSWDDLRQVNTTLKSKNPNVWPICQPINN
                     LAKTMPWVWTSGGDFWDKDILPTKADFTNSVVSNVYKFEEEWSQKQWRNTDELNDTTT
                     LQLMISRKCVGLIYSTDEILQLEANDPKT"
BASE COUNT          686 a         1071 c         1026 g          600 t
ORIGIN      
        1 gtccaagcct ggcgcaccga gatctcctgg ccgatcaaac tgacggcggc cgccggctag
       61 aagggcgcca tccgatttga cttttccctg ttcgatctcg cagaggtcca ccaacgtgaa
      121 gggtagggtg cttagcgcct gttgaacccc gaattcggtc gacccggaac cgccagagcc
      181 cgactgcgac gctcgatgcg caacgcccac gcagacgctt tagcatctga ctatgagcgc
      241 tcgtgcggaa cagatccgct tcggtgtgat cggcatcaat catggccaca tttacggcat
      301 ggccaaccag ttgctggatg caggagcgga actcgtcgcg tttttcgctc ccgaagatga
      361 cctcgctgcc gagttcagca acaagttccc ccaggcgcgc cgcgcgaggt cgaaggcgga
      421 agtcctcgag gatcgctcgc tggcgctgat cgcgagcgca ggcattccga atgaacgcgc
      481 tccgctcggc gtcgaaacca tgcggcacgg caaggactac ctgagcgaca agcccgggtt
      541 tacgacgctc gaacagctcg aggaggcacg cagcgtccag cgacagacgc gtcgcatcta
      601 ttcagtctgc tacagcgagc gagtccagaa ccgcgcgact gtccgcgctg gcgagctggt
      661 cgaagcgggc gcgatcggtc gtccgctcca gactgtcgga cttgggccgc accgtctgaa
      721 cgctcccagc cgaccgaaat ggttcttcga gcacgatcgt tacggcggga tcctcaccga
      781 catcggctca catcaggtgg accaattcct ctttttcacg ggctcgactt ccgcagaagt
      841 ggtggcatcg caggttggca acgtggcgca ccctgagtac ccgggtctcg atgattttgg
      901 cgatatgctg ctgcgcggcg acggcggcac gggctatgtg cgcgtcgatt ggttcacgcc
      961 cgatggcctg agcgcgtggg gcgatggtcg gctgacgatc ctcggcaccg atggcttcat
     1021 cgagatccgc aagaacgtgg atatcggcgg ccgcgcgggt ggtgaccacc tgttcatcgt
     1081 cgaccagcgc gagacgcgct acatggactg caagaacgtc gaattgccat tcggaccgcg
     1141 gctgctcgat gacgttcgca atcgcaccga aaccgcgatg ccgcaggctc acaccttcct
     1201 ggcatccgag ctcgccctga acgcacaggc tcaggcgcgc tccatcacgc gccagagcgt
     1261 cgccgcgtga gcacccccga tcgcctgcgc gtcgcggtcg tcggcgctgg gatcgggcgc
     1321 tcgcatatca ggggctacca ggccgttggg gaccgcttcg agctgcttgc gctgtgtgac
     1381 atcgacgctg cacgcggaaa gccagttgcc gaggagcacc acatccctcg cttcgttacg
     1441 gatttcgccg agctgctgcg gatggacgac ctcgatgtca tcgacatctg cacgccgcca
     1501 cacctgcatt tcgagcagat ccagcaggta ctcgcctcgg gcaaacacgc gatctgcgag
     1561 aaaccactcg tgagctcttt gcgcgaggtc gacgggctga aacgcctcga agcgagctcg
     1621 ggtcggcgca tcatgccgat ttttcagtat cgattcggcc acggcatgca gaagctcaag
     1681 ctgctgctcg agcgcgggat cgctggcaag ccctacctgg caactgtcga gaccgcgtgg
     1741 cgacggcggc ccgcgtatta cgatgttccc tggcgaggga agtgggcgac tgaacgcggc
     1801 ggcgccctgc tgggtcacgc catccacaac cacgatctgc tgtgctacgt gttgggcgcg
     1861 ccgaagcgac tgtgggcgta tgccacgacg cgcgtcaacg caatcgaagt cgaggactgc
     1921 gcggcgatcg cgatggaaat ggccgatggc tcggcagctt ccagcggcgt gacgctcggc
     1981 tcggcggaag agatcacccg ccatcgcttc tcgttcagtg gcctggtggc tgagagcaac
     2041 acacggccgt acaccaattc aggtgatccg tggactttcg ttggcgattc acccgacaga
     2101 gacgaggaaa tcgctctgac cctggcgagg ttcaagccgc tgcccgagca ctacccgggc
     2161 cagttctatc gcttctacga tgcgattcgg tcgggcggcg agctgcctgt cacgctggca
     2221 gatgcgcgct tgtcgatcga gctgatcacc ggcatgtact actcgtccga gacgggtcag
     2281 gcggtcacct ttccaatcgg cgccgatcac ccgcggtaca ccagctggat ccccgccgga
     2341 aagtaacgaa acgttgccat acagcgaagc gtggcacgag ttacgctgat taataggtag
     2401 cgcgcacttt ccgaccggag gtgaccttac ggtgctctca gccaggctta ctcatctcgc
     2461 gacccttgtg acggtcgccg ccttgctggc tggttgcgga acaggcagca gcaattacac
     2521 acccgcgggc ggcgcggcag cgccgacgca accggctggt gcagcagcgg gccaacccac
     2581 aaccgccgcg gcggccgcgg gcggccagaa gacgcacctc gtctacttca acgcccgcgg
     2641 cgcggaagcg gccgaacgcg cgctggtcaa tcgctacatg caggaccacc ccaacatcga
     2701 gatcgaatat ctctcgacga cgtcgctcaa tggtcctagc gacacggacg cgatcgcgaa
     2761 cctgattttc aacattcagg cgaagaagac tatcgatatc gccaagatcg aagtcagccg
     2821 cacgccgctt gacctcatgg cggccagtgc gatccaggaa ctgaacgcgg tcggcggcga
     2881 cgccgtgaag aaccagctga aaggtttgct caacaataac tacgtcgaca tcaacaatgg
     2941 cacctgggcc attccctacg agttcgatcc cttcggctac gtctacaacg ccgacatgta
     3001 caaggacgcc ggcctcaatc cggacaatcc gccgaagagc tgggatgacc tgcgccaggt
     3061 caataccacg ttgaagtcca agaacccgaa tgtctggccg atctgccagc cgatcaataa
     3121 tctggccaag actatgccct gggtctggac ttcgggggga gatttctggg acaaggacat
     3181 cttgccgacg aaagccgact tcaccaactc cgtcgtttcg aacgtctaca agttcgaaga
     3241 ggagtggtcg cagaaacagt ggcgaaacac cgacgagctg aatgacacaa cgacgctcca
     3301 gctgatgatt tcgcgcaaat gcgtgggctt gatttacagc accgacgaga tcctgcagct
     3361 cgaggctaat gatccgaaaa ctg
//
LOCUS       DATGEM010000346         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1145652, whole genome shotgun sequence.
ACCESSION   DATGEM010000346 DATGEM010000000
VERSION     DATGEM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145652"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1296)
                     /gene="earP"
                     /locus_tag="VKQ27_11010"
     CDS             complement(175..1296)
                     /gene="earP"
                     /locus_tag="VKQ27_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HLY89501.1"
                     /translation="MRWDLFCAVVDNLGDIGVCWRLARQLAAEHAAEVRLWVDDLATF
                     RQLAPGLDLAASRQRLGGIEVCAWATPFAPVKPADIVVETFGCELPASYVQAMKARTR
                     PPVWINLEYLSGEPWVGRYHGLPSPQPPLTKYFFFPGFASDTGGALMERGLNARRVAF
                     QADAAEQARFLSGIRVAARAPDELLVSLFCYPQAPMPALFDAWASGSRPIRVIAFADT
                     PAAQALVAAGLASGARRGPLSGQIVPFLTQDDYDRVLWACDWNFVRGEDSFMRAQWAA
                     RPFVWQVYPQQEQNHRIKMQAFLARYLGASVMGSSAGLVDLWTAWNGLLPPARLGPAW
                     TASLATMGGLSRLASAWPERLEALGDLAGNLARFCAERV"
     gene            complement(1397..2122)
                     /gene="fabG"
                     /locus_tag="VKQ27_11015"
     CDS             complement(1397..2122)
                     /gene="fabG"
                     /locus_tag="VKQ27_11015"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HLY89502.1"
                     /translation="MKVKTALVTGGSGAIGSAICRALAAQGLRVIVHANHRLAEAQRL
                     AAELQAGGAAAQAIAFDVTDRDAAARVLASIEEQAPVQVLVNNAGVHDDAPLAGMSGS
                     QWDRVIDVSLNGFYNVTHPLLLPMIRTRWGRIINISSVAGVIGNRGQVNYSAAKAGLH
                     GATMSLALELASRGITVNAIAPGVIDSPVIRQAFPKQTIEALVPMKRAGTTDEVAALV
                     AFLASAQAGYITGQVISINGGMA"
     gene            2258..>3018
                     /locus_tag="VKQ27_11020"
     CDS             2258..>3018
                     /locus_tag="VKQ27_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLY89503.1"
                     /translation="MQSSVHQTELLLFFTLLQLAVIVVAARLAGELAVRLSQSRAVGE
                     IIAGLVLGPSLFGLLLPELFQFVFRSAPPEPMTILSQIGLILLMFQIGLEFDFAHLKA
                     GQNRNAVLRISVMGLALPFALGLLFGWVSHPYLAAQINLLGYALFVGTAFSITALPIL
                     GRILMELGLTRTRVGVIAISSAAINDVIGWLMLAVVTALTAAAFSPASFVLKIGLILI
                     YIAVCWWGIRPLLLRLLHYCGADGGSLSPNLVGIL"
BASE COUNT          521 a         1028 c          922 g          547 t
ORIGIN      
        1 gcaggttctt caatttcatc ctcatcacgg cggcattgcg gccgcccttg ttgtattcgg
       61 cgcgctgcac gaccatcggg tccttgccga ccatgatcac gttgccggcg cgcacatcct
      121 gagcctgtat catcgtctat cctgaaagag agaggggtgc gaaaaagcgc gtgattatac
      181 ccgttccgcg cagaaccggg ccaggtttcc ggccagatcg cccagtgctt cgaggcgctc
      241 cggccatgcg cttgccagac gggaaaggcc gcccatggtc gccagactcg ctgtccaggc
      301 cggccccagg cgggcgggcg gaagcaggcc attccacgcc gtccacaggt cgaccagccc
      361 agcggacgaa cccatgaccg acgcgccgag gtatcgggcc aggaaggcct gcattttgat
      421 gcggtgattc tgttcctgct gcggatacac ctgccacacg aaaggtcggg ccgcccattg
      481 ggcgcgcatg aacgagtcct cgccgcgcac gaagttccag tcgcatgccc acagcacccg
      541 gtcgtagtcg tcctgcgtga gaaacggcac gatctgcccg gacagcgggc cgcgccttgc
      601 gcccgaggcc agtcccgcgg cgaccagtgc ctgtgccgcc ggcgtatccg caaaggcgat
      661 cacccggatc ggccgactgc ccgacgccca ggcgtcgaac agtgccggca tcggcgcctg
      721 cggatagcaa aacagggaga ccagcagttc gtccggcgcg cgtgcggcca cgcggatgcc
      781 cgaaaggaaa cgggcctgtt cggccgcatc cgcctgaaag gccacgcgcc gggcattcag
      841 gccgcgctcc atcagcgcgc cgccggtatc ggacgcgaaa cccggaaaga aaaaatactt
      901 ggtcagcggc ggctgcggcg acggcaggcc gtgataacgg cccacccagg gctcaccgct
      961 caggtattcg agattgatcc agaccggcgg ccgggtgcgc gccttcatcg cctgcacgta
     1021 agacgccggc aattcgcaac cgaaggtttc caccacgatg tcggcgggtt tgaccggcgc
     1081 gaaaggcgtg gcccatgcgc aaacctcgat gccaccgagc ctctggcggg aagccgcgag
     1141 gtcaagcccg ggtgcaagct ggcggaatgt tgccaggtcg tccacccaca gccgcacctc
     1201 ggccgcatgt tccgccgcga gctggcgcgc caggcgccag cacacgccaa tgtcgccgag
     1261 gttgtcgacg acggcacaga aaaggtccca gcgcatttaa gtacagtgaa cggtgaatcg
     1321 tgaatggtga ttggtaaaaa gcctctctaa accgattttc gccaatcacc catcactcat
     1381 caccaatcac cgcctttcac gccattccgc cattaatcga aatcacctgc cccgtgatat
     1441 agccggcctg cgccgaggcc aggaacgcca cgagcgccgc tacctcatct gtcgtgcccg
     1501 cgcgcttcat cggcaccagt gcctcaatgg tttgctttgg aaaggcctgc ctgatcaccg
     1561 gcgaatcgat gaccccgggc gcaatcgcat tgaccgtgat gccgcgcgag gccagttcca
     1621 gcgccagcga catggtcgcg ccgtgcaggc cggccttcgc cgccgagtaa ttgacctggc
     1681 cgcgattgcc gatgacgccg gctaccgagg aaatattgat gatcctgccc cagcgcgtgc
     1741 ggatcatggg cagcagcagc gggtgcgtca cgttgtaaaa gccgttcagc gatacgtcga
     1801 tcacgcgatc ccattgcgac ccgctcatcc cggccagggg cgcatcgtca tgcacgccgg
     1861 cattgttgac cagcacctgc accggcgcct gttcctcgat cgacgcaagc acccgggccg
     1921 cagcatcgcg atccgtcacg tcgaaggcga tcgcctgcgc cgccgcgccg ccagcctgca
     1981 actccgcggc caggcgctgc gcctctgcga gcctatggtt ggcgtgcacg atcacccgca
     2041 ggccctgtgc cgccagggcg cggcagatcg ccgatccgat cgccccgctg ccgccggtta
     2101 ccaatgccgt tttcactttc atcttcgtcg tcctgatttg cggaccggat tttacccggt
     2161 cgatgttatg cttcgccggc gcgcaccgcg gcatggtgcc atagcggggg catggcgcta
     2221 tcgcaggcgg gtctcgtcta aagggagaaa tgatcgcatg caatcgagcg tgcaccaaac
     2281 cgaactgctg ctgttcttca cgctgctgca gcttgcggtg atcgtggttg ctgcgcggct
     2341 cgcaggggag ctcgccgtgc ggctgtcgca gtcccgcgca gtgggcgaga ttatcgccgg
     2401 gctggtgctc ggaccttcgc tgttcgggct actgctcccg gagctgttcc aattcgtgtt
     2461 ccgctccgct cccccggaac cgatgacgat cctcagccag atcggattga tcctgctgat
     2521 gttccagatc gggctggaat tcgacttcgc ccacctgaag gccggccaga atcgcaacgc
     2581 ggtgctgcgc atttccgtca tgggactggc gttgccgttc gcgctcggcc tgctgttcgg
     2641 ctgggtctcg cacccctatc tcgccgcgca aatcaacctg ctcggctacg ccctcttcgt
     2701 cggcaccgca ttttcaatca ccgcgctgcc catcctcggc cgcattctga tggagctcgg
     2761 cctgacccgg acccgcgtcg gggttatcgc catcagctcg gctgcgatca atgatgtgat
     2821 cggatggctg atgctggccg tggtcacggc gctcacggct gcggcattct cgcccgccag
     2881 cttcgtcctg aaaatcggcc tgatcctgat ttacatcgca gtgtgctggt gggggatacg
     2941 cccgctcctg ctgcgcctgt tgcactactg cggcgcagac ggcgggtcgc tgtcgccaaa
     3001 cctggtcggt atcctgat
//
LOCUS       DATGEM010000347         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1164010, whole genome shotgun sequence.
ACCESSION   DATGEM010000347 DATGEM010000000
VERSION     DATGEM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164010"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VKQ27_11025"
     CDS             complement(<1..994)
                     /locus_tag="VKQ27_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89504.1"
                     /translation="MTAHAIAGPEVARYASAARPRRISLPPGWPFLAMLILSPLWYVL
                     GLGSFIWILLAAPMFAHLLMTRSWRAPRGFILWLFFLLWVLLSAVGLQTTGNAFTYLF
                     RTGAYVAAGLGFLYLVNVDEKDLPTDKILVALTVFWIVIVGGGFLALAIPHFSFSSPF
                     QKVLPGSFLSNRYLHDLVHPSLSQAQALGGVRAIGHAITRPAAPFPYTNQWGGTYAFL
                     IPFVILTMRRLRSRRRRIALAVLLVISLVPFVISINRGAWLSLAVAALYAWIRFAVRR
                     NLRSSVGMLVLLGVVVLTVLVTPLRGVVLARLGHHGSASLRLELYHQSAQSALQS"
     gene            complement(997..1920)
                     /locus_tag="VKQ27_11030"
     CDS             complement(997..1920)
                     /locus_tag="VKQ27_11030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89505.1"
                     /translation="MTSPASWPSVSAVIPTRNRPDLLRRAVQSVLDQRYPGALECVVV
                     FDGESPRRPDVTVPPGRSLRTISNDRSPGLAGARNAGAAACDGDLLAWCDDDDEWLPE
                     KLTQQAEAMRRFPDAAVATCGICVCVGNRRFTRSPASDVVPREALLRSRQMWIHSSTL
                     LIRRDCFFGEIGPVDEAIPASYGEDYEWIIRASQATPIVASPEPLVRVYWQNSYYADR
                     WDVMISGIQYHLDKHPELAADARNAARMYGRLAFAYGASGMSRQARRWASRSIRLDWR
                     QPRGYLAMLVSARIISPRQVMALAHSQGRGI"
     gene            complement(1917..2855)
                     /locus_tag="VKQ27_11035"
     CDS             complement(1917..2855)
                     /locus_tag="VKQ27_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89506.1"
                     /translation="MLDRLLGELPGFAAVGELRYIWSEALTDNRLCGCGARFLACPFW
                     LDVGMRAYGGWDRIDSQQAVSLERSIARHRHIPLLAMGGSMPSRYAARIQEYLAMLTP
                     LYQAISDASGASVIVDSTKDAAYAHVLRRMPGVDFRLLHLVRDSRGVAWSWAKVVKSQ
                     EVVGRDHYMRQFAPSVTGLRWMAFNLIVESLGWFGVPRLLLRYETLVADPAAALAAIA
                     AFAGKPVPAAEMVFLDGDGARLGVHHTVAGNPMRLTAGRIDIRMDDEWRTSMPFGQRA
                     GVTALTWPLLRHYAGSPPRELASATHDLARPEGSSR"
     gene            complement(2915..>3328)
                     /locus_tag="VKQ27_11040"
     CDS             complement(2915..>3328)
                     /locus_tag="VKQ27_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89507.1"
                     /translation="AGPPAYLPRYSLGASSLRWLLYNGMTQAFRSTRVPYVMFRYEDL
                     VHTPRDLVGLALRHAGVTGAAAEPDYIEGNRVRLAANHTVEGNPMRFVNGELDLRADE
                     QWQREMPSRQRRLVTALTLPMLTAYGYPMNGKRPA"
BASE COUNT          662 a         1074 c         1099 g          493 t
ORIGIN      
        1 gcgattgcag ggcgctctgg gccgactgat ggtagagctc gaggcgcagc gacgcgctgc
       61 catgatgccc tagtcgcgcc agaactaccc cccgcaaggg cgtgaccagg accgtcagca
      121 cgacaacgcc cagcagcacc agcattccca cgctcgatcg aagattccgc cggacggcga
      181 accggatcca ggcatagagg gccgcgacgg caaggctgag ccacgccccg cggttgatcg
      241 agataacgaa cggcaccagc gaaatcacca gcaggaccgc aagggcaatt cgccggcggc
      301 gggagcgcaa ccggcgcatg gtgaggatga cgaaggggat caggaacgca taggttccgc
      361 cccactggtt ggtgtaagga aatggggccg ctggccgggt gatcgcgtgc ccgatcgctc
      421 tgactccgcc gagcgcctgg gcctgagaaa gactcgggtg gaccaagtca tggaggtacc
      481 ggttggagag aaaggagccc ggaaggactt tttgaaatgg agaagagaac gaaaaatgcg
      541 gaatggcaag ggcaaggaac ccgcccccga caataacgat ccagaagaca gtcagagcaa
      601 cgagaatctt gtctgtgggc agatcttttt catcgacatt taccagataa aggaatccta
      661 gccctgccgc cacgtaagcg cccgtccgga acagataagt gaacgcgtta cctgttgtct
      721 gcagcccgac tgccgagagc aatacccaca agagaaagaa aagccagagg atgaatcctc
      781 tcggagctcg ccacgagcgg gtcatcagga ggtgcgcaaa catcggcgcg gccaggagta
      841 tccagatgaa cgagccgaga ccgagcacat accacagcgg agagaggata agcatggcga
      901 ggaacggcca tcccggcggc aggctgatgc gccgagggcg cgccgctgag gcgtaccgcg
      961 ccacctccgg cccggctatt gcgtgcgccg tcatacctag atacccctcc cctgggaatg
     1021 tgccagcgcc atcacctggc gcggcgaaat gatgcgagca gagacgagca tcgccaggta
     1081 tcccctcggt tgccgccagt cgagccgtat ggagcggctc gcccaccggc gggcctggcg
     1141 ggacataccc gaggcgccgt aggcaaacgc cagccgacca tacatgcgcg ccgcgttgcg
     1201 cgcgtcagca gccagctcag ggtgcttgtc cagatggtac tggatgcctg agatcatcac
     1261 atcccaccga tcggcgtagt acgagttctg ccagtacacc cggaccagtg gctcgggact
     1321 ggcgacgatc ggggtggcct gcgaagcccg gatgatccat tcgtagtctt cgccgtaact
     1381 ggcgggtatg gcctcgtcga ctggtccgat ctcgccgaag aaacagtctc gcctgatgag
     1441 cagcgtgctg gagtggatcc acatctggcg cgagcggagc aaggcttcgc gcggcacgac
     1501 gtccgaggca ggagagcggg tgaagcgccg gttgcctacg catacgcaga tgccgcacgt
     1561 ggcgacggcc gcgtcgggaa agcgccgcat cgcctcagcc tgctgcgtca gcttctccgg
     1621 gagccactcg tcgtcgtcat cgcaccacgc cagaaggtcg ccatcgcagg cggcagcgcc
     1681 ggcgttccgc gcccccgcga gaccgggaga gcgatcgttg ctgatcgtcc tgagcgagcg
     1741 gcccggcgga accgtcacgt cgggccgccg gggagactcg ccgtcgaaca cgacgacaca
     1801 ttcgagcgca cccggataac gctggtcaag gacgctctga accgccctgc gcagcagatc
     1861 gggcctgttc cgcgtgggaa tgaccgcgct caccgacggc caggatgccg ggctggtcat
     1921 cggctcgacc cttcgggcct ggctaggtca tgcgtggcgc ttgccagctc gcgtgggggc
     1981 gaacccgcat agtggcgcag cagcggccag gtgagcgcgg tcaccccggc tcgctggccg
     2041 aatggcatgg acgtgcgcca ttcgtcgtcc atccggatgt caatccggcc ggccgtcaat
     2101 cgcatcgggt tcccggctac ggtgtgatgc acgcccaaac gcgcgccgtc accgtccagg
     2161 aacaccatct ccgccgcggg gaccggcttc ccggcgaacg cggcgatggc ggcgagcgca
     2221 gcggccgggt ccgccacgag cgtctcatac cgcagcagca gacgcggcac gccaaaccat
     2281 ccgagggatt caacgatgag attgaaggcc atccatcgca ggccggtcac cgacggcgcg
     2341 aactgcctca tgtagtggtc ccggccgacc acctcctgag atttgacgac cttcgcccag
     2401 gaccacgcca cccctcggct gtcgcggacc agatgcagca gccggaaatc tactcccggc
     2461 atccgccgaa ggacgtgcgc atacgcggca tccttggtcg aatcgacgat aacgctcgcg
     2521 ccgctggcat cgctgatggc ctgatacaga ggcgtcagca tggcgaggta ttcctggatc
     2581 cgggcggcgt agcgagacgg catcgaaccg cccattgcca gcagggggat gtggcggtgg
     2641 cgggctatcg agcgctccag cgagacagcc tgttgcgaat cgatccggtc ccagccgcca
     2701 taagctcgca ttccgacgtc gagccagaat gggcaggcga ggaaacgagc gccgcatccg
     2761 cacaggcggt tgtcggtcag tgcctcactc cagatgtacc ggagctcgcc tacggccgcg
     2821 aaccccggca gctctccgag cagccggtcg agcaaggtac tgccgctgcg tcctgcgccg
     2881 ccgatgacta gcacggtccg gcgcccggtg ctggtcatgc cggacgcttg ccgttcatcg
     2941 ggtacccgta cgccgtcagc atcggcagcg tcagggccgt taccaggcgg cgctgccttg
     3001 acggcatttc ccgttgccat tgctcatcgg cccggagatc aagctcgcca tttacaaacc
     3061 gcatgggatt gccctcgacg gtgtggttgg cggcgagccg gacgcggttg ccctcgatgt
     3121 agtccggttc tgcggccgcg ccggtgacgc ccgcatgccg cagggcaaga ccgaccaagt
     3181 cgcgcggggt atgcacgagg tcctcatagc gaaacatcac gtacgggacg cgcgtcgatc
     3241 tgaaggcctg agtcatcccg ttgtaaagca gccagcgcag cgatgacgcg ccgaggctgt
     3301 agcgaggaag ataggcgggc ggacctgc
//
LOCUS       DATGEM010000348         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_465734, whole genome shotgun sequence.
ACCESSION   DATGEM010000348 DATGEM010000000
VERSION     DATGEM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465734"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VKQ27_11045"
     CDS             <1..187
                     /locus_tag="VKQ27_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89508.1"
                     /translation="ATRIFAWAVVICVVAAALAVASFALWFALILIPVVVVAGAIAWL
                     AFRFQLWRAGGRWPPRA"
     gene            complement(177..482)
                     /locus_tag="VKQ27_11050"
     CDS             complement(177..482)
                     /locus_tag="VKQ27_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLY89509.1"
                     /translation="MQFLVMLYDGTDAEAPARRAATRPAHLEGIKGNVANGKLLFGGP
                     LFNDAQKPTGSFLLVECADRAELDAIIANDPYTKGNVWQKVEVKPTRLVVRNGTITP"
     gene            complement(467..1483)
                     /locus_tag="VKQ27_11055"
     CDS             complement(467..1483)
                     /locus_tag="VKQ27_11055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89510.1"
                     /translation="MLRLSVLDQSTVVTGRSPDTSIRESLALAKHCEALGYTRYWCAE
                     HHNSASQAGTAPEILISAIAATTSRIRVGSAGVMLPHYSSLKVAEQFRVLEAIAPGRI
                     DLGLGRAPGSDGRTAFALNPLAETAADHFPAQVRDLLAWLSGTKLVEGHPFRDIIAQP
                     GGPTLPEVWMLGSSDYGPQVAAYFGIPFCFAHFITDGRGVEQAMAIYREGYRPSERHP
                     KPYAALCVWALAADTEEEAARLFSSREMWRLGRDRGVYEALPSPEEAAAQHYSEGELA
                     RIERLRARALYGTPSVVGEKLRALASEHGVDEIAILTTLHDPEGRRRSYTLLAQEFGL
                     CSSS"
     gene            complement(1694..2737)
                     /locus_tag="VKQ27_11060"
     CDS             complement(1694..2737)
                     /locus_tag="VKQ27_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HLY89511.1"
                     /translation="MARYDSILGTVGNTPVVRINKLAPAGVNLFVKVEAFNPLGSVKD
                     RLALGVIEAAERSGELKPGQTVVEATSGNTGIGLAMVCAAKGYPLVVTMAESFSVERR
                     RLMRFLGAKVVLTPASGRGMAMVTKANELAAMNGWFLTRQFENEANADMHSRTTAREI
                     IDDFKDTHLDYWVTGYGTGGTLKGVARVLAKESPNTKIVACEPADAAMLSSGEPQQRN
                     ADGSPAAAHPAFKPHPMQGWAPDFIPKLTADAVDSGVLHKILRIPGPDAQKYSKELAA
                     KEGIFVGLSAGATFAGALQICAEAPKGANILAMLPDTGERYLSTPLFGDIPADMTEEE
                     VAISKSTPSAQMP"
     gene            2823..4310
                     /locus_tag="VKQ27_11065"
     CDS             2823..4310
                     /locus_tag="VKQ27_11065"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLY89512.1"
                     /translation="MNDTAISTATETFDVLIVGAGISGIGGAYHLTKQCPGTRFVVLE
                     SQESFGGTWRTHKYPGIRSDSDLHTFGYRFKPWRSAPIATAAEILHYMGEVIEENDLA
                     RHIRYRHAISSASWSGEDNLWTITATRLDTGEVKRFRANFLWMCQGYYRHAEGYTPHW
                     DGMESFNGRIVHPQAWPDDLDYHNKTVVVIGSGATAATLIPAIAPDCKHVTMLQRSPT
                     YFRAARNADELAELLRDLQIDETWIHEIVRRKILRDQDLFTRKTFEQPEIAKKELIAG
                     VRANLGPDYDVEKHFTPSYRPWRQRIAFVPDGDLFQGIRSGKASVVTDEIERFTETGI
                     LLKSGTRLDADIIITATGFHLSVLGDIEFTIDGKPLVFSDTVTYRGMMFTGIPNMAWV
                     FGYFRASWTLRADLVADFVCRLLNHMRQRGARRVEVVLRPEDRDMPLLPWIDTESFNP
                     GYVLRGMHLLPKRGDKPEWQHTQDYWKEKDEFPAIDFDGAAFLYA"
BASE COUNT          800 a         1590 c         1226 g          807 t
ORIGIN      
        1 cgctacgcgg atcttcgcgt gggctgtcgt catctgcgtg gtggcggctg cgctggccgt
       61 cgcatcattc gcactgtggt tcgcgctgat cctgatcccg gttgtggtgg tggccggcgc
      121 gatcgcctgg ctggcgttcc gcttccagct ctggcgcgcc ggaggccgct ggccgcctag
      181 ggcgtgatcg tcccgttgcg taccaccaga cgcgttggct tcacctccac cttctgccag
      241 acgttcccct tcgtgtaggg atcattggcg atgatcgcgt cgagctcggc gcgatctgca
      301 cactccacca gcagaaacga gccggtgggc ttctgcgcgt cattgaacag aggtccgccg
      361 aacagcagct taccattggc gacgttgccc tttatcccct ccagatgcgc ggggcgtgtc
      421 gcagctcgcc gagccggcgc ctcggcatcc gtgccgtcat agagcatcac gaggaactgc
      481 ataatccaaa ctcctgtgcc agcagtgtat acgatcgccg ccgcccctcc ggatcatgca
      541 gcgtggtcag gatggcgatc tcatcgaccc cgtgttcaga cgccaaggcc cggagcttct
      601 cccccaccac cgacggcgtg ccatatagcg cccgtgcccg caatcgctcg atgcgcgcca
      661 gctcgccctc ggagtagtgc tgcgcggccg cctcttccgg cgatggtagc gcttcataga
      721 cgccgcggtc gcgacccagc cgccacatct cccgcgagct gaacaaccga gcggcctctt
      781 cctcggtatc tgccgccaaa gcccacacgc acagcgctgc gtagggcttc ggatggcgct
      841 cgctgggacg atacccctcc cgatagatcg ccatcgcctg ctcaacgccg cgcccgtcgg
      901 tgatgaaatg tgcgaagcag aatgggatgc cgaaatacgc tgctacctgc gggccataat
      961 ccgaactgcc gagcatccaa acttccggta gcgtcggccc gcctggctgc gcgatgatgt
     1021 cgcggaacgg atgcccttcc accagcttgg tgcccgaaag ccacgccagc agatcgcgca
     1081 cctgcgctgg gaaatggtcc gctgcggtct ccgccagcgg gttcagcgcg aacgccgtac
     1141 gcccatcgga ccccggcgct cgcccaagcc ccagatcgat gcgccccggc gcaatcgcct
     1201 ccagcacgcg gaactgctcg gctaccttca ggctggaata gtgcggcagc atcactcccg
     1261 cggatcccac cctgatgcgc gatgtggtcg cggcaatcgc gctgatcagg atttccggcg
     1321 ccgtccctgc ctggctggcc gagttatggt gttccgcgca ccagtaccgc gtgtaaccaa
     1381 gcgcctcgca atgcttcgcc agcgccaggc tctcgcggat cgaagtatcc ggcgagcgcc
     1441 cggtcaccac cgtcgattgg tcaagcacgg aaaggcgaag cattccctgg ctccacggtc
     1501 atgccaaaca ttatggtgct gatgagcggg aaagcgagag ggcagacgct gcctatggca
     1561 ctattggtca gaaaacgcgt cctgccctaa gcgccaatcc cgtgccaact gtcattgcga
     1621 gcgaagcgaa gcaacctcga acgagattgc ttcgtcactc cgttcctcgc aatgacagta
     1681 gacacaaacg ccattacggc atctgtgcgc tcggcgtcga cttggagatc gcaacctcct
     1741 cctcggtcat atccgccgga atgtcaccaa acagcggcgt gctgagatag cgctcgccgg
     1801 tatccggcag catcgccagg atattcgcgc ccttcggcgc ttcggcgcag atctgcaacg
     1861 cgccggcgaa cgtcgccccc gcgctgagac caacgaagat gccttccttc gccgccagct
     1921 ccttgctgta tttctgcgcg tcgggccccg gtatccgcag gatcttgtgc agcacgccgc
     1981 tgtcgactgc gtccgccgtc aacttgggga tgaaatcggg cgcccacccc tgcatcggat
     2041 gtggcttgaa agccggatgc gcggccgccg gcgagccatc cgcattccgc tgctgaggtt
     2101 cgccgctgct cagcatagcc gcatccgccg gctcgcacgc gacaatcttg gtgttgggag
     2161 attccttcgc cagcacccgt gccaccccct tcagcgtgcc gcctgtgccg tatccggtca
     2221 cccaataatc cagatgcgta tccttgaagt catcgatgat ctcgcgcgcc gtcgtgcgcg
     2281 agtgcatatc cgcattggcc tcattctcga actgccgcgt caggaaccag ccgttcatcg
     2341 ccgcgagctc gttggccttg gtcaccatcg ccatcccgcg tcccgaagcc ggcgtcagca
     2401 ccactttcgc gccgaggaac cgcatcagcc ggcggcgctc aacgctgaaa ctctccgcca
     2461 tcgtcaccac cagcggatag cccttcgcgg cacagaccat cgccagaccg ataccggtgt
     2521 tgccgctggt cgcctccacg accgtctgtc caggcttcag ctcgccgctg cgctccgccg
     2581 cctcgatcac ccccagcgcc agccgatcct tcaccgaacc gagcggattg aacgcctcca
     2641 ccttgacgaa caggttcaca cccgccggag cgagcttgtt gatccgcacc accggcgtgt
     2701 tgccgacggt tcccagaatg ctgtcgtagc gcgccatggc tgcctccctg ttgtctcccg
     2761 gcgcatatag gcatccccgc accgcgcttg ctagatatgc cgcagagggg agcatcattc
     2821 ccatgaacga tacagcaatc tcgaccgcga ccgagacctt cgacgtcctg atcgtcggcg
     2881 ccggcatttc cggcatcggc ggcgcatatc atctcacgaa gcaatgcccc ggcacgcgat
     2941 ttgtcgtgct ggaatcgcag gagagcttcg gcggcacctg gcgcacgcac aaatatcccg
     3001 gcatccgctc cgacagcgac ctgcacacct tcggctatcg tttcaagccc tggcgcagcg
     3061 cgccgatcgc gactgccgcc gagatcctcc actacatggg cgaagtgatc gaggagaacg
     3121 atctcgcccg tcacatccgc tatcgccacg cgatctcctc tgcaagctgg tccggcgagg
     3181 ataatctttg gaccatcaca gccacgcgcc tcgataccgg cgaagtgaag cggttccgcg
     3241 cgaatttcct gtggatgtgc cagggttatt accgtcacgc ggaaggctac acgccgcact
     3301 gggacggcat ggaaagcttc aatggccgca tcgttcatcc gcaggcctgg cccgacgacc
     3361 tcgactacca caacaagacg gttgtggtga ttggctctgg cgctaccgcc gcgacactga
     3421 tccctgcgat cgcgcccgac tgcaagcacg tcacgatgct gcagcgctcc cccacctatt
     3481 ttcgcgccgc gcgcaacgcc gacgaacttg ccgaactcct gcgcgatctc cagatcgacg
     3541 aaacctggat ccacgagatc gtccgccgca agatcctccg cgaccaggat ctgttcacac
     3601 gcaagacctt cgagcagccc gagatcgcca agaaagagct catcgctggc gtacgcgcta
     3661 acctcggtcc cgactacgac gtggaaaagc acttcacccc cagctatcgc ccctggcgcc
     3721 agcgcatcgc cttcgtccct gacggcgacc tcttccaagg catccgctct ggcaaagcct
     3781 ccgtcgtcac cgacgagatc gaacgcttca ccgaaaccgg catcctgctg aaatccggca
     3841 cccggctcga cgccgacatc atcatcaccg ccaccggctt ccacctgagc gtgctcggcg
     3901 acatcgagtt caccatcgat ggcaagcccc tcgttttctc cgacaccgtc acctatcgcg
     3961 gcatgatgtt caccggcatc ccgaacatgg cctgggtgtt cggctatttc cgcgcaagct
     4021 ggactctgcg ggccgacctc gtcgccgatt tcgtgtgccg gctgctcaac cacatgaggc
     4081 aaaggggcgc ccgccgcgtg gaagttgttc tccgccccga ggaccgcgac atgccactcc
     4141 tcccctggat cgacaccgag agcttcaacc ccggctacgt cctccgtggc atgcacctgc
     4201 tgcccaaacg cggcgacaag cccgaatggc agcacacgca agactactgg aaggaaaaag
     4261 acgagttccc ggcaattgat ttcgacggcg ctgcgttcct gtatgcatga ggagcgttct
     4321 gccgtcctcc ccccgcgtca tgccgggagc agggccaaca tgtaccacca ttgttgatac
     4381 aagcctctac ccaaccccca ccgccttctt ctccaccagc ccc
//
LOCUS       DATGEM010000349         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_698289, whole genome shotgun sequence.
ACCESSION   DATGEM010000349 DATGEM010000000
VERSION     DATGEM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698289"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1089)
                     /gene="galE"
                     /locus_tag="VKQ27_11070"
     CDS             complement(100..1089)
                     /gene="galE"
                     /locus_tag="VKQ27_11070"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010668280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HLY89513.1"
                     /translation="MAQRFLVTGGAGYVGSHLVLALVERGDSVVVIDNLRTGHRAAVP
                     DGAQLAVIDLADTNAVDALLSEGPWDAVFHFAALTQVGESMRLPFHYLIDNGMHGMRL
                     IEACVRHGVSRFVLSSTCAVYGTPATVPIPEDAPQNPESPYGEGKRFIERALYWADRI
                     HGLRFAALRYFNAAGADPAGRIGEDHRPETHLIPLVIDAALDRRPELDVYGNDYPTPD
                     GTCIRDYVHVNDLADAHIRAVDRLERGSITLNLGIGSGHSVLQVIDAVERVSGRRVPH
                     RMCPRRPGDPAALVASAQHAQQELRWRPRFADLDEIVRTALLWREAHPNGYGD"
     gene            complement(1097..3295)
                     /locus_tag="VKQ27_11075"
     CDS             complement(1097..3295)
                     /locus_tag="VKQ27_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine kinase"
                     /protein_id="HLY89514.1"
                     /translation="MLDRPAGTTEASRTARRERQPHEREPDTAALSAATVLAILRRRK
                     WPFLASTVLCPLLAFVALSQLTPKYTASGTLLYDAAEYNVRELQSILRVDPITDAVMA
                     TQAEVLRGMPVVEQVANRLNLFANPDFNPALRPTPWWRRALHRMVAGEHVAAPDIMGP
                     QLDPSRNATLAAVHAALVVTPLKASHVLEVSFTARDPVLAAAAANHAMDAYVKARLGA
                     KYGAVMRARDWLEQRAKELRVEVHHYEDEIAKYRAEHGLVEGMHAGLDSEQISLLSEN
                     LARARNTLAEAEGRLDAASGRAGAAAQAAIASSVAPLRARQGQLTAELQSKLGRLGPN
                     HPDIQATRVQLAETDRSIAAEIARVVAATDADVRADRDRVAKLQTDLEDVRAQLQRNA
                     QAQVPLNAMQRDADASRSLLLSVLERIQQTTQQSAIEAPDAHEISLALPPSQPSFPRT
                     GSWMAGAVASGVLFGLLLVYLWELTDASFHTGDDIRSTLGLPCLALIPRVGRRELKGM
                     AVEEYAARKPLSPFAEQLRALRAGLSLWPNRPRVVAVTAARPREGKTVVTEALGRLAA
                     TNGERVIVVDCDIRQPNPGTGAGLVDCLLDHVTLADVVRRDPATGMAYIPCGRRDTNA
                     LGLLMSASMERLLQVLREEYDLVLLDTPPAEAITDARIVAGLADATLFCVRWRATSRG
                     VATHALGLLEEAHANVVGAALTQVDINVHVRSGYADAEVYHPRNGGYFRE"
     gene            3461..3982
                     /locus_tag="VKQ27_11080"
     CDS             3461..3982
                     /locus_tag="VKQ27_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HLY89515.1"
                     /translation="MLTGCSGPGSRLPMLPDGSPGSYALGPGDQVRIIVYGEDNLTGE
                     FRVNDAGNIALPLVGAVHAAGLTSSGLETAVGAALRRDNLVRNPSVAVEVIAYRAIYV
                     LGEVNKPGQYAYQPGMTVVTAVAVAGGFTYRAVQGYAAVVRTADGTAVEGRASRQSFV
                     RPGDVITVLERRF"
     gene            3966..4781
                     /locus_tag="VKQ27_11085"
     CDS             3966..4781
                     /locus_tag="VKQ27_11085"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HLY89516.1"
                     /translation="MSAGSDRIRRFAVLLALLGVAGARDAPAPGPLHIIGGDNAGCLA
                     GAVRLPADGDGFQTIHLGRSSFWGAPVTIAGIEELGRTAHAAGLGDLYVEDVARPRGG
                     SLPGGHVGHQLGLDADVGLDVSRKPALSATEREAVELPSMVRADQRGIDPAYWSPQVA
                     TLLHLAAELPGVDRILVNAAIKQQLCDTVHGDRSWLRMIRPWYDHSAHMHIHFRCPPG
                     QRDCVDIPPQPAGDSCDATLQWWFDQLSAPPQPAKPYRPPPLPVACKAVMEAP"
     gene            complement(4785..>5870)
                     /locus_tag="VKQ27_11090"
     CDS             complement(4785..>5870)
                     /locus_tag="VKQ27_11090"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HLY89517.1"
                     /translation="DDFWRWREILYRFLAILTPDDVQAIAAQLYVECLLNGYTSIAEF
                     HYLHNAPDGSRYADKAEMSRRIKAASRDAGIGLTLLPVLYRHSQFGGAPPTEGQRRFI
                     LSLDDHADLCATVGDGVAPHSLRAVTPEDLASAIAIAGDKPIHIHIAEQQKEVTDCIA
                     WSGTRPVAWLLDHAQVDQRWCLVHATHIDAAECTRLAASGAIAGLCQTTEANLGDGIF
                     PLRDYLAEGGRFGIGSDSNVSTSPVEELRWLEYLRRLETRTRNVTTNLYTRAVAGGAQ
                     ALGRAIGAIAPGTCADLVVLDTEHPSLVGHSGETLLDAWLFSGNATPVRQVMVSGHWV
                     VRDGVHRVQRQIATEFARTMRQLADRL"
BASE COUNT          995 a         1813 c         2042 g         1020 t
ORIGIN      
        1 gtgccggtgt cagagaagac gagagggtat ccgttccaga gcgccggcca caacaaagat
       61 gcagcggcga gcaggatagg ggcgactgcg ctcgatcgct cagtcgccat agccgttggg
      121 atgcgcctcg cgccatagca gcgcggtgcg cacgatctca tccaggtcgg cgaatcgtgg
      181 ccgccagcgg agctcctgtt gcgcgtgctg cgctgatgcg accagtgccg cgggatcgcc
      241 cgggcggcgc gggcacatcc gatgcggtac gcgacgtccc gacacacgct cgacggcatc
      301 gatcacctgc agcaccgagt ggccagagcc gatgccgaga ttaagggtga tgctgcctcg
      361 ctccagccgg tccaccgcgc ggatatgcgc gtcggccagg tcgttgacgt gtacgtagtc
      421 ccggatgcag gtaccatccg gcgttgggta gtcgttgcca tagacgtcga gttccgggcg
      481 gcggtcgagc gcggcgtcga tcaccagcgg gatcagatgg gtttccggac gatggtcctc
      541 gccgatgcgg cccgccgggt cggcgccggc agcattgaaa tagcggagcg cagcgaagcg
      601 caggccatgg atgcgatcgg cccagtagag cgcgcgctcg atgaatcgct tgccctcgcc
      661 atagggcgat tcgggatttt gcggcgcgtc ctccggaatc ggcaccgtcg ccggcgtgcc
      721 gtacaccgca caggtcgacg atagaacaaa gcgcgatacg ccgtgtcgga cacaagcctc
      781 gatcagccgc atgccgtgca tgccgttgtc gatcaggtag tggaacggca gtcgcatgct
      841 ctcgcccacc tgggtgagcg cggcgaaatg gaacacggcg tcccacggtc cttcggaaag
      901 cagggcgtcc acggcattgg tgtcggccag gtcgatgacg gcaagctgcg cgccgtcagg
      961 gactgccgcc ctatgaccgg tgcgcagatt gtcgatcacc accacgctgt cgccccgttc
     1021 aaccagagcc agcaccaggt ggctgccgac ataaccggcc ccgccggtga caagaaaccg
     1081 ttgcgccatc gttgttctac tcccggaagt agccgccgtt gcgcggatgg tacacctccg
     1141 catcggcata gcccgagcgc acatggacgt tgatgtccac ctgcgtcaag gctgcgccaa
     1201 ccacgttggc gtgcgcttcc tccaggagcc caagagcgtg cgtggcgaca ccgcgcgacg
     1261 tggcgcgcca ccgcacacag aacaatgtgg cgtcggcaag gccggcgact atgcgtgcgt
     1321 ccgtgatcgc ttcggcaggc ggcgtgtcga gcagcaccag gtcgtactcc tcgcgcagta
     1381 cctgcagcag acgctccatc gatgcggaca tcaagaggcc aagcgcgttg gtatcgcgcc
     1441 ggccacacgg aatgtaggcc atgccggtgg ccggatccct gcgaaccaca tcggcgagtg
     1501 ttacgtggtc aagcagacag tccaccaggc ccgcccctgt gccagggttc ggttggcgga
     1561 tgtcgcagtc caccacgatg acacgttcgc cgttggtcgc agccaggcgc ccaagagcct
     1621 cggtgaccac ggtcttgcct tcgcgtgggc gcgccgccgt gacggcgacg acacgcggcc
     1681 ggttgggcca gagcgacagg cccgcacgaa gcgcgcgaag ctgctcggcg aatggcgaga
     1741 gcggcttgcg tgcggcgtat tcctcgacag ccatgccctt cagctcgcgc cggccgacgc
     1801 gtgggatcag cgcaaggcag ggcaggccaa gggtgctgcg gatgtcgtcg ccggtatgga
     1861 agctggcatc ggtaagctcc cacagataga ccagcagcaa accgaacagg acgcctgatg
     1921 cgacggcgcc cgccatccac gatccggtgc gcgggaagct gggctgcgac ggcggcagcg
     1981 ccagcgatat ttcgtgggcg tcaggcgctt cgatcgctga ttgctgagtg gtctgctgga
     2041 tacgctcgag taccgacagc aacaggctgc gcgatgcatc ggcgtcgcgc tgcatggcat
     2101 tcaggggaac ctgcgcttgc gcgttccgct gaagctgggc gcgcacatcc tccagatcgg
     2161 tctgcaactt ggcgacccgg tcgcggtcag cgcgcacgtc ggcatcggtg gcggcaacca
     2221 cgcgggcgat ttcggctgcg atcgaacggt cggtttcggc aagctgcaca cgcgtcgctt
     2281 ggatgtccgg gtggttgggt ccgaggcggc cgagcttgga ctgcaattca gccgtcaact
     2341 gtccctggcg agcgcgcaaa ggcgcgactg acgaagcgat cgcagcctga gccgcggcac
     2401 cagcgcggcc gcttgctgca tcgagccggc cctcggcttc agccagcgtg ttgcgggcgc
     2461 gggccaggtt ctccgacaga aggctgatct gttcgctgtc caggccagcg tgcatgcctt
     2521 ccaccagacc gtgctctgca cggtacttgg cgatctcatc ctcgtaatga tggacttcga
     2581 cccggagttc cttggcgcgc tgctcgagcc aatcgcgggc tcgcattacg gcgccgtact
     2641 tggcgcccag tcgggccttc acgtaggcat ccatcgcatg attggccgcg gctgcagcca
     2701 gcacaggatc gcgtgcggtg aacgacacct ccagcacatg cgatgctttg agcggggtaa
     2761 cgaccagagc agcgtgcacc gctgccagcg tcgcgttgcg tgaaggatcg agctgcgggc
     2821 ccatgatgtc tggtgcggct acgtgttcgc cggcaaccat ccggtgcaaa gcgcggcgcc
     2881 accagggcgt tggacgcagc gcgggattga agtcgggatt ggcgaacagg ttcagccggt
     2941 tcgcgacctg ctccaccacc ggcatgccgc gcaacacctc agcctgggtt gccatcaccg
     3001 catcggtgat cggatccacc cgcaggatgc tttgcagctc gcggacattg tactccgctg
     3061 catcatacaa aagggtgccg ctggcggtgt acttcggagt gagctgactg agcgcgacaa
     3121 atgccagcaa cgggcacagc accgtcgagg caaggaacgg ccatttgcgc ctgcgcagga
     3181 tggcaaggac ggtggcagcg ctcagtgcgg cagtatcggg ctcccgctcg tgcggctgcc
     3241 gttcgcggcg cgccgtgcgg gaggcttcgg tcgtgcctgc cggtcggtcg agcatcgccg
     3301 tcctgcatcg ggggaagaca gcccaccggc ttattggcga gcgcgtgtcc cgatggagat
     3361 aggattaccg cctgcgtctt aatctccggc taattcctgc cacctcatag tggccggcat
     3421 ggatgcgcgg cgttgtgtcc ctgcgctgat gattctgttg atgctgaccg gttgtagcgg
     3481 gccgggcagt cgtctgccga tgctgcccga cggcagtccc gggtcgtatg cgctcgggcc
     3541 gggcgatcag gtgcggatca tcgtgtatgg cgaagacaat ctgaccggcg aattccgggt
     3601 gaacgatgcc gggaatatcg cgcttccgct ggtcggcgcc gtacacgcgg cggggctgac
     3661 atcgagcggg ctggaaaccg cggttggtgc agcgttgcgt cgtgacaacc tggtccgcaa
     3721 tccatcggtg gcggtggagg tgattgccta tcgcgcgatc tatgtactgg gcgaggtgaa
     3781 caagccaggg cagtacgcat accagccagg gatgacggtg gtgaccgcgg tggccgtggc
     3841 aggcggcttc acctatcgcg cggtgcaagg ttatgctgcg gtcgttcgca ccgcggacgg
     3901 gacggcggtg gagggcaggg cgagcaggca gtccttcgta cggccgggcg atgtcatcac
     3961 ggtgcttgag cgcaggttct gatcgcatcc gccggtttgc cgtgctgctc gcgttgttgg
     4021 gcgttgctgg cgcacgcgat gcgccggcgc cggggccgtt gcacatcatc ggtggcgata
     4081 atgccggatg cctggccggc gcggtgcggt tgccggccga cggcgacggg tttcagacca
     4141 tccatctggg gcgaagctcg ttctggggcg cgccggtgac gatcgccggt atagaggaac
     4201 tgggaagaac ggcgcacgcg gccggccttg gcgacctgta cgtcgaggac gttgcccggc
     4261 cacgcggcgg gtcgttgcct ggtgggcacg tcgggcatca gttgggcctg gatgccgatg
     4321 tcggactgga cgtgtcgcgg aagcctgcgc tgagtgcgac ggagcgtgag gcggttgaac
     4381 tgccgagcat ggtgcgagcc gatcagcgcg gcatcgatcc agcgtactgg tcgccgcagg
     4441 tcgcgacgct gctgcatctt gcggccgaac tgcccggcgt tgaccgcatc ctggtgaacg
     4501 cggcgatcaa gcagcagctt tgcgacacgg tgcacggcga tcgatcgtgg ctgcggatga
     4561 tacgaccatg gtacgaccac tcggcgcata tgcatattca tttccgctgc ccgccgggtc
     4621 agcgcgattg cgtcgacatt ccgccgcagc cggctggcga tagctgcgac gcgacgctgc
     4681 aatggtggtt cgaccagttg agcgccccgc cgcagccggc caagccgtac cgcccgccac
     4741 cgttgcctgt tgcgtgcaag gcagtgatgg aagcgccctg actgtcagag ccgatcagcg
     4801 agctggcgca tcgtgcgggc gaattcggtc gcgatctgcc gctgtactcg atgcacgcca
     4861 tcgcgcacga cccaatggcc ggagaccata acctggcgaa cgggtgtcgc gttgccggag
     4921 aacagccagg cgtcgagcag cgtttcgccg gagtggccga caagggacgg gtgttcggtg
     4981 tccagcacca cgagatcggc gcaagtgcct ggggcgatcg cgccgattgc gcggccgagt
     5041 gcctgcgctc cgccggcgac agcgcgcgta tataagtttg tcgtcacgtt gcgggtgcgc
     5101 gtttccaggc ggcgtaggta ttccagccag cgcaattcct cgaccggcga ggtgctgaca
     5161 ttggaatcgc tgccgatgcc gaatcggccg ccttcggcaa gatagtcacg cagcggaaag
     5221 atcccgtcac cgagattggc ctcggtggtt tggcacaggc cggcaattgc gccgctcgcc
     5281 gcgaggcggg tgcattccgc ggcgtcgatg tgcgtcgcgt gaactaggca ccagcgctga
     5341 tctacctgcg catggtccag cagccaggca actgggcgcg tgccgctcca ggcaatacag
     5401 tcggtcactt ccttctgctg ctcggcgatg tggatgtgga tgggtttgtc gccggcgatc
     5461 gcgatcgccg atgccaagtc ctccggcgtg acggcgcgca atgagtgagg cgcgacgccg
     5521 tcgcctactg tggcacacag gtccgcatgg tcatcaagcg agagaatgaa acggcgctgg
     5581 ccttctgtcg gtggcgcacc tccgaattgc gaatgccggt acagcacggg gagaagcgtg
     5641 aggccaatgc cggcatcgcg agaggcggcc ttgatacggc gggacatctc ggctttgtcc
     5701 gcgtagcgcg atccatctgg tgcattgtgc agatagtgga attcggcaat cgaggtgtag
     5761 ccattcagta gacactcgac atagagctgt gcggcgatgg cttgcacatc atcgggggtg
     5821 aggatggcaa ggaagcgata cagtatctcg cgccaccgcc agaaatcgtc
//
LOCUS       DATGEM010000350         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1027507, whole genome shotgun sequence.
ACCESSION   DATGEM010000350 DATGEM010000000
VERSION     DATGEM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027507"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VKQ27_11095"
     CDS             complement(<1..630)
                     /locus_tag="VKQ27_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89518.1"
                     /translation="MISLTDPWLADALARWAPHGMIADWLDDQGAAIADVLRLFAGTA
                     PEFIDGLHLPVVLAFAHAHGLAAEADKAKDLREMAPVTPQGVLDMIEQGARTRQRAED
                     LHCFAGYARKDVPFYNAVCFCERRIACWSLDGYVPPPAPVAILHLEPSPYQRPRPFLS
                     ARCEKCGRIAWSVYPGPEHRDWKRAVAACFGCSGIIPPEPPVWGVSWLRG"
     gene            complement(738..1364)
                     /locus_tag="VKQ27_11100"
     CDS             complement(738..1364)
                     /locus_tag="VKQ27_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89519.1"
                     /translation="MTRNWRIYYIDENALLHIFVQRDMPRFLSLTLPTNLPPGAKVLD
                     VRHSWDRKAFGFRVEHPSFDPVPDGALIPEAGPLEWNIVRVLPDYDNPERKGPPAYRL
                     LDAALEEADRLRGVFTDLCNDRAVVEAAREQGAKEEEKRLLGLIEEYMQAWKKPGDRL
                     ADLVVSAMGVLASAVRHKDQDYASAMDRIIEELMLGLPGEVGDVFPAA"
     gene            complement(1361..2452)
                     /locus_tag="VKQ27_11105"
     CDS             complement(1361..2452)
                     /locus_tag="VKQ27_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89520.1"
                     /translation="MANIGTLASHGPAKLTLEHPDGPTLQWALASAEVRPENITREVN
                     LGLGFPAELEYVGQRITVSGRVIPQQHPQDGTIYTHSTTLPAPTVPRDEITAEAVAEL
                     RGILNNLRRICDRPDTLVSPRIDQIVARLLAILGLSPGERAFVQDAADANAAGRMGDL
                     DDVLAIFADWLEDGARPEIAGRMRRLRFADGDVLILTYSPEDAEAAGAVHTAMDRIRD
                     SLPTKVDCLAISLPESVKIEEIDAVHMRTLGWVRTAEHDALFANLQSEVKDEPECVVD
                     LDRKHGGGLTSGNIIRLIRRLKQENAQLREVSKQKSIALLEAMGPVRGMAESLAATER
                     ASPSRKTPADLGMPDTLMGHPIIVEDDQP"
     gene            complement(2445..2645)
                     /locus_tag="VKQ27_11110"
     CDS             complement(2445..2645)
                     /locus_tag="VKQ27_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89521.1"
                     /translation="MIFRRVVGGKTAADLACLQLEVQADAKDLLAKGWIVDQRSPTVT
                     VYPESGTIEFTFAATPPEKRNG"
BASE COUNT          437 a          908 c          912 g          483 t
ORIGIN      
        1 cccgcgcagc cacgagacgc cccacaccgg cggctcggga gggatgatcc ccgaacaccc
       61 gaagcacgcg gccacggcgc gcttccagtc gcgatgctct ggaccggggt agaccgacca
      121 tgcgatgcgc ccgcacttct cgcagcgggc gctgaggaag gggcgcggcc gctgataagg
      181 gctgggttcg agatggagaa tggcaacggg ggccggcggc ggaacgtatc cgtcgaggct
      241 ccagcaggcg atgcgccgtt cgcagaagca gaccgcattg taaaagggaa cgtccttgcg
      301 tgcataaccg gcgaagcagt ggaggtcttc ggcccgttgt cgcgttcttg ctccctgctc
      361 gatcatgtca aggacgccct gcggcgtgac cggcgccatc tcacgcagat ccttcgcttt
      421 gtccgcctcg gcggcgaggc cgtgcgcgtg ggcaaaggcc agcaccacgg gcaagtgcag
      481 gccgtcgatg aattccgggg cggtcccggc gaacaggcgc agcacgtcgg caatggccgc
      541 gccctggtcg tcgagccagt cggcgatcat gccgtggggc gcccagcggg ccagcgcgtc
      601 ggcgagccag gggtcggtga gggagatcat ggctgatgca actccgctgc atcggtggcg
      661 tgggattcaa attcccacgg ttcgcgaacc tggatcggtt cgcccgcctg ggccgcctga
      721 agggccgcgc cgatgcttca tgcggccggg aacacgtcgc caacctcgcc tggaaggcca
      781 agcataagtt cctcgatgat gcgatccatc gcagaggcgt aatcctgatc cttgtgcctg
      841 acggccgatg ctagaacgcc catcgccgag acgaccagat ccgcaaggcg gtcgcccggc
      901 ttcttccatg cttgcatgta ttcctcgatg agccccaaca gtcgcttctc ctcctccttc
      961 gccccctgct cgcgcgccgc ctccacaacc gccctatcgt tgcacaggtc ggtgaagacg
     1021 ccgcgcaggc gatcggcctc ctcaagcgcg gcgtccagca ggcggtaggc gggcgggccc
     1081 ttccgctccg ggttgtcgta gtcgggcagg acgcggacga tgttccactc cagaggcccc
     1141 gcctcgggga tcagggcgcc gtccggtacg gggtcgaacg agggatgctc gacgcggaag
     1201 ccgaacgcct ttcggtccca ggagtgccgc acgtccagca ccttcgcgcc gggcggcagg
     1261 ttggtcggga gcgtcaacga gagaaagcgc ggcatgtccc gctgcacgaa gatgtgcaag
     1321 agggcgttct cgtcgatgta gtagattcgc cagttgcgag tcatggctgg tcgtcctcca
     1381 cgatgatcgg gtggcccatg agcgtgtctg gcatgcccag gtcggccggg gtcttacgcg
     1441 atggggaggc tctttccgtt gccgccagac tctctgccat gccgcgaacc ggccccatag
     1501 cctccagcag agcaatgctt ttctgtttgg agacttcccg caattgggcg ttctcctgct
     1561 tcagccggcg gatcaggcgg atgatgttcc cgctggtcag accgccgccg tgcttgcggt
     1621 cgagatcgac cacgcattcc ggctcgtcct tgacctcgct ctggaggttg gcgaagagcg
     1681 cgtcgtgttc ggccgtgcgc acccagccca gcgttcgcat gtggaccgcg tcaatttctt
     1741 cgatcttcac gctctcgggt aggctgatcg ccaggcaatc gaccttcgtg ggcagagaat
     1801 cccgtatccg gtccattgcc gtgtggaccg ccccggccgc ttccgcatcc tcgggcgaat
     1861 aggtcagaat cagcacgtcg ccgtccgcga accgcaggcg gcgcatgcgc ccggcgatct
     1921 cgggccgggc gccgtcctcc agccagtccg cgaagattgc cagcacgtcg tcgaggtcgc
     1981 ccatgcggcc ggcagcgttg gcgtcggcgg cgtcctgcac gaaggcgcgt tcgccggggg
     2041 acaggcccag gatcgccagc agtcgcgcaa caatctggtc gatgcgcggg ctgacaagcg
     2101 tgtccggccg gtcgcaaatg cgccgaaggt tgttcaggat tccccgcagc tccgcaaccg
     2161 cttccgccgt gatctcgtcg cgcggcacgg tcggggccgg caaggtggtg gaatgcgtgt
     2221 agatcgtccc gtcctgcggg tgctgctgcg gaatcacccg gcccgacacc gtgatccgct
     2281 ggccgacgta ctccagttcg gctggaaatc cgagccccag gtttacctcg cgggtgatgt
     2341 tctccggccg gacctcggcg ctcgccagcg cccactggag cgtcgggccg tcgggatgct
     2401 ccagcgtcag tttcgccggt ccatgcgagg ccagcgtgcc gatgttagcc attgcgtttc
     2461 tccggagggg tcgcggcaaa ggtaaactcg atggttcccg actcggggta aacggtcacg
     2521 gtgggcgaac gctgatcgac aatccatccc ttcgccagca ggtctttagc gtcagcctgg
     2581 acttccagtt gcaagcaggc aaggtcggcg gccgtttttc cgcccaccac gcggcgaaag
     2641 atcatacgaa cctcatcgtt tcccagggac cgccgcaggg atgccacttc tcccactcga
     2701 ccctggagtc gagcaggtag tccgccgccg tgcagggctg
//
LOCUS       DATGEM010000351         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1441508, whole genome shotgun sequence.
ACCESSION   DATGEM010000351 DATGEM010000000
VERSION     DATGEM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441508"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VKQ27_11115"
     CDS             complement(<1..1103)
                     /locus_tag="VKQ27_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLY89522.1"
                     /translation="MLACCAPEAPPPGLSDAEVAAALGIMAPLAPLYRALLSKQEGQR
                     GAPVVILQVGDSHTANDSFSGRLRELFQAQFGDAGRGVLPPGVPHPYYRPARVSVYTQ
                     GWSVVSSYRGEPGPFGITGLRQHADLPAQMTLSVDDPGELALVEIEVLRQPGGGTLDV
                     NGTAIATAAPAPQAVWLKVPMAAGTTALTVSARGDGPVDLLAWSIGRGRPGVIYANLG
                     TIGASADLLDQWDQSILHQDIAHLAPSMIVVAFGTNEGFRDSTDPTAFAADFAARLRD
                     LHAAAPGAALVVLGPPDAYRRRGKRSSAPVACGDPNWTEPPNLGAIREAERSVAAQEH
                     AWFWDWRAAMGGPCSMMRWAATVPPYAAPDHVH"
     gene            1229..1945
                     /locus_tag="VKQ27_11120"
     CDS             1229..1945
                     /locus_tag="VKQ27_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HLY89523.1"
                     /translation="MLQRRGLLFAFGLAALPARIACATDTRTRILVVGDSQAQGLAAG
                     LQRQFRRDPSWRVVDRSRIATGLCSPSRFDWPAAVPAIGEEERGAIAVVMFGANDRPP
                     IRMHGSINEKLEATFCTTYGAHVRAIAAALKHSCAGVIWVGHPIVRDPVYAEDMALLN
                     RLYAEQSLAAGADWFPSWPLFTDADGNFAPYGKGADGETTRLRADDGVHLTGPGYDVL
                     ARALLPVVEIYQPVATHAPA"
     gene            1951..2481
                     /locus_tag="VKQ27_11125"
     CDS             1951..2481
                     /locus_tag="VKQ27_11125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLY89524.1"
                     /translation="MLDCVVLGDSIAVGVGQARPDCQTFAVSGISSDHYVQIFPGAPQ
                     ARTAIISLGVNDSESVATADNLARLRGRVVADTVYWLVSGTNPHIRDAVHAIAARFGD
                     RLIDVVPLAGADHIHPDRTGYARLAAETGGTDDGTSAPASAYQDSPQQQPVYQAIPGL
                     KVWNGPDNLNGVPVKQ"
     gene            complement(2492..>2721)
                     /locus_tag="VKQ27_11130"
     CDS             complement(2492..>2721)
                     /locus_tag="VKQ27_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89525.1"
                     /translation="DNGDPLMFIEAIPADHTRQFIEDALVYSWQYAAAMHVPAASLDA
                     LAAGHYPRLVRANARAKFGGDRACSMTAAQR"
BASE COUNT          468 a          971 c          841 g          441 t
ORIGIN      
        1 atgtgcacgt gatccggtgc ggcgtagggc ggcacagtcg cggcccagcg catcatgctg
       61 cacgggccac ccatcgccgc ccgccagtcc cagaaccagg catgctcctg cgcggccacg
      121 ctgcgctccg cctcgcggat agcgcccaga tttggcggtt cggtccaatt gggatcgccg
      181 caggcgaccg gcgcgctact ccgtttgccg cgcctgcgat aggcatcagg cggcccgagc
      241 acaaccagcg cagcccccgg cgccgccgca tggaggtcgc gcaatcgtgc agcgaaatcg
      301 gccgcgaaag cggtcggatc agtgctgtcg cggaatcctt cgttagtgcc gaatgccaca
      361 acgatcatcg aaggtgccag atgcgcgatg tcctggtgca gaatcgactg gtcccactga
      421 tccagcaggt cggcgctggc accgatggtc ccaaggttag cgtagatcac tccgggccgg
      481 ccgcggccga tgctccaggc cagcaggtcg actggcccat cgccgcgcgc actcaccgtc
      541 agcgcggtcg tccccgccgc catcggaacc ttcagccaca ccgcctgcgg tgccggcgca
      601 gccgtcgcaa tggcggtgcc gttcacatcc agcgtgccgc cgcccggctg gcgcagtacc
      661 tcgatctcga ccagcgccag ctcgcccggg tcatcgaccg acaacgtcat ctgcgccggc
      721 aggtccgcat gctgccgcag tccggtgatc ccgaagggcc caggctcgcc gcggtaactg
      781 ctgaccacgc tccagccctg cgtgtaaacc gacacccgcg ccggccggta atacggatgc
      841 ggcacaccgg gaggcaacac accgcggccg gcatcgccga actgtgcctg gaacaactcc
      901 cgtagccgcc cggaaaaact gtcgttggcc gtgtggctgt cgccaacctg cagaatgacg
      961 acgggggccc cgcgctgtcc ctcctgtttg gacaggagcg cccggtacag gggcgccagg
     1021 ggcgccataa tacccagtgc agcggcgacc tcggcatcgg aaagtccggg cggcggcgcc
     1081 tctggagcgc agcaagcaag caacagcgcg atcgcgccgg agaaaaaatg tcgtcggcac
     1141 gatgagaaca taaggctttg gttgctcctg cgaatccgtg ctgttaattt agcacgacaa
     1201 agcctctgaa attctacgga tcctgaccat gttgcagcga cgcggtctgc tgttcgcttt
     1261 cggccttgcc gcgctgcccg cgcgcatcgc atgcgcaacc gacaccagga cccgcatcct
     1321 cgtcgtgggt gactcgcagg cacaaggtct tgccgccggg ctgcagcgcc agttccgccg
     1381 cgacccgagt tggcgggtgg tcgatcgatc ccggatcgcg accggactct gctcgccgtc
     1441 ccgcttcgat tggcccgccg ccgtccccgc catcggcgag gaagagcgcg gcgccatcgc
     1501 tgtcgtcatg ttcggcgcca acgaccgccc gccgatacgc atgcacggct ctatcaatga
     1561 aaagctcgag gcgacgttct gcaccaccta cggcgcccat gtgcgcgcca tcgcggctgc
     1621 gctgaagcat tcctgcgccg gcgtcatctg ggtcgggcat ccgatcgtcc gtgatccggt
     1681 ttacgccgag gacatggcgc tgctgaaccg cctctatgcc gagcagtcgc tggccgccgg
     1741 cgccgactgg ttcccgtcct ggccgctgtt cactgatgcc gatggcaatt tcgctcctta
     1801 cggcaagggc gccgacggtg agacaacccg cctgcgtgca gacgacggcg tgcacctcac
     1861 cggccccgga tacgacgtgc ttgccagagc tctgctgccc gtcgtcgaaa tctaccagcc
     1921 cgtggccacg cacgcgccgg cgtaatcggt ttgctcgatt gcgtcgttct cggtgacagc
     1981 atcgcggtcg gggtcggcca ggccaggccg gactgccaga cgttcgcagt ctccggtatc
     2041 agctccgacc actacgtgca gatcttcccc ggcgcgccgc aggcccgcac agcaatcatt
     2101 agcctggggg tcaatgacag cgaaagtgtc gcaactgctg acaacctcgc taggctgcgc
     2161 ggacgcgtcg tggccgatac cgtctactgg ctggtctcag gcaccaaccc gcacatccgc
     2221 gacgcggtcc acgcgattgc cgcacggttc ggcgacaggc tcatcgatgt ggttccactg
     2281 gcaggcgccg accacatcca ccctgaccgc accggctacg cccggctcgc agcggaaacc
     2341 ggcggaaccg acgatggcac ttcagcccct gcctcggctt accaggattc tccgcagcag
     2401 cagccagtct accaggccat tccgggactg aaggtctgga acggaccgga caatctgaat
     2461 ggtgtgccgg taaagcagta aggatgccga cctaccgctg ggccgctgtc atactgcacg
     2521 cgcgatcacc cccgaacttc gctctggcgt tggcccgaac cagccgcgga tagtgccccg
     2581 ccgccaacgc atcgagactc gccgccggca cgtgcatcgc cgcggcgtac tgccacgaat
     2641 agaccagcgc atcctcgatg aattgccgcg tgtggtccgc cggaatcgcc tcgatgaaca
     2701 tcagcggatc gccgttgtct c
//
LOCUS       DATGEM010000352         9574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_511315, whole genome shotgun sequence.
ACCESSION   DATGEM010000352 DATGEM010000000
VERSION     DATGEM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511315"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1306)
                     /locus_tag="VKQ27_11135"
     CDS             complement(95..1306)
                     /locus_tag="VKQ27_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89526.1"
                     /translation="MPTEAAPESAYAWLRLAISVALSTIGGISLWSVVVVLPAVQTEF
                     GVDRATASLPYTLAMVGFALGGVMMGRMADRWGIMWSTLIGAAALSAGYIAAAASTSL
                     WQFALAHGVLVAFGGAATFGPMVADISHWFTRRRGIAVAIAASGNYLAGTAWPPLVQH
                     YDQSIGLRQTLLGIGILCAVTMIPLALTLRRRPPVHVGTVTTSVSTASLGLSPGALQT
                     TLVLAGFCCCVAMSMPQVHIVAYCGDLGYGITRGTEMLSLMLGLGIVSRVGSGFLADR
                     LGGLPTLLIGSIGQMLALLLYIGFSTLYPLYAISALFGLVQGGIVPCYAIIVREYMPA
                     REAGMRFGIVIMATLFGMAFGGWISGVIFDATGSYHAAFANGALWNLLNACIAVFLLS
                     RARTARRWATA"
     gene            complement(1373..2110)
                     /gene="hypB"
                     /locus_tag="VKQ27_11140"
     CDS             complement(1373..2110)
                     /gene="hypB"
                     /locus_tag="VKQ27_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HLY89527.1"
                     /translation="MEHEHVHADGTRHSHHHDHEHRTTVIALNTRILAKNDALAARNR
                     AWFAGREILAINLVSAPGAGKTTLLERTIRDLDGQTNIGVLEGDQATTLDSDRVRAAG
                     ASVVQVNTGTGCHLEADQVARGLNELRPPTRSIVMIENVGNLVCPAMFDLGERAKVVL
                     LSVTEGDDKPLKYPHMFRAASLMLLNKVDLLPHVDFDVQRALACAREVNPDMSVLRLS
                     ARTGEGMTAWYEWLQREAKAASEAAFI"
     gene            complement(2143..2385)
                     /locus_tag="VKQ27_11145"
     CDS             complement(2143..2385)
                     /locus_tag="VKQ27_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HLY89528.1"
                     /translation="MHELSLTREIVSIACNAASGQRVHMISVEVGKLSCVSPDALAFC
                     FDVVAQGTLAEGARLNIRRTNGDELHVVTLEVEEAV"
     gene            complement(2385..3404)
                     /gene="hypE"
                     /locus_tag="VKQ27_11150"
     CDS             complement(2385..3404)
                     /gene="hypE"
                     /locus_tag="VKQ27_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HLY89529.1"
                     /translation="MKTVTLSHGGGGKAMKDLIDVVFVSVFDNPLLAPLDDQARVPLA
                     SLAQYGDRLAFTTDSFVVDPLEFPGGDIGKLAVCGTINDLAVGGAIPLYLSCAAIIEE
                     GLPFDLLERMARSMALAARDAGVDIVTGDTKVVQRGACDKLFLTTTGIGVIRGDVVLG
                     AQYARPGDVVLVNGVLGDHGAAILAARGDLALDAPIDSDCAALHGLVETLLVAAPNTR
                     FMRDATRGGVATVLNEIAHAAQCGIELSETAVPLREPVKAFCEILGLDPLYLANEGRL
                     VAVVPPDEAARALAAMRGHPLGLGACRIGQIVERRPGLVVMHTVLGGDRIVDMLVGDQ
                     LPRIC"
     gene            complement(3401..4510)
                     /gene="hypD"
                     /locus_tag="VKQ27_11155"
     CDS             complement(3401..4510)
                     /gene="hypD"
                     /locus_tag="VKQ27_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HLY89530.1"
                     /translation="MKYVDEFRDRDKAAALLHAIEAVTEEIRPAKALQIMEVCGGHTH
                     AIFRYGIEAMLPDAVELVHGPGCPVCVLPMGRVDDCVAIATQPGVILVTFGDAMRVPG
                     SQGSLMQAKANGADIRMVYSPTDALAIARNNPEREVVFFGLGFETTMPSTALTVLEAE
                     RDGISNFSVFCNHITIVPTLKTILDSPDLRIDGFVGPGHVSMVIGSAPYEFIARQYRK
                     PLVISGFEPLDLLQSVWMVLRQIADGRAEIENQYARVVASGGNAAAQRAIDAVYEPRE
                     HFEWRGLGSIDHSGVRFREAYARFDAERRFVVPGVRVTDPAACQCGEVLKGALKPQQC
                     RVFGTDCTPETPMGALMVSSEGACAAYYRYAAKTV"
     gene            complement(4507..4755)
                     /locus_tag="VKQ27_11160"
     CDS             complement(4507..4755)
                     /locus_tag="VKQ27_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HLY89531.1"
                     /translation="MCLGIPAQIVCIDDADRMLATAEVGGVRRQVNIICIVDDHHPPE
                     SCVGDWVLLHVGFAMSRIDAAEAEATLGVLTELGEMAP"
     gene            complement(4765..6954)
                     /gene="hypF"
                     /locus_tag="VKQ27_11165"
     CDS             complement(4765..6954)
                     /gene="hypF"
                     /locus_tag="VKQ27_11165"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HLY89532.1"
                     /translation="MQQSEIRVRGRVQGVGFRPAVWRIARELGLSGDVRNDPGGVLIR
                     VAGEEAAINGLLQRLRDGQPRLARIERMDCRAIEGSCAPGFRIVASAGGEPRTEITPD
                     AAVCAECAREVLDRGQRRAGYAFANCTQCGPRFSILRQIPYDRSATTMAPFGLCSACR
                     QEYEEPGDRRFHAEAIACPACGPRLQFDVEIAAQVLQSGGIVALKGIGGYQLACDATN
                     AGSVLRLRDAKHRETKPFALMARDAEIVHRYCAVGAEELRQLSSPEAPIVLMRVRGER
                     LPDAVAPGVKTLGVMLPTTPLHLLLLQDINRPVVMTSGNLSDEPQIIDDGEARERLGG
                     IADVLISHDRVIVTRVDDSVVRVVGGIARLVRRSRGYAPAAIALPAGFEGAPDLLAAG
                     AEQKNTFCLLTDGKAFLSQHIGDLEDARTFDAYRGAIEHFAELFDHTPTTVAADAHPE
                     YLSSKYARECGLSLIEVQHHHAHVAACLAENGRPLDAPPVLGIVLDGLGWGDDGTIWG
                     GEFLLAHYRQARRLAMFKPVAMPGGVAAVREPWRNLYAHLAAAGLLQRPHAMLDAMIR
                     AGINSPLASSCGRLFDAMAAALGICRERQGHEGEAASRLEALVRPCDLTGEAAYPMVV
                     DGWIDPAPLWQAVMNDLQAQVPPEVMAARFHKGVAAAVVAMTRRLHGFDTVALSGGCF
                     QNAVLLELIGSQLRDAGYRVLSHSTVPTNDGGLALGQAAIAAARLMA"
     gene            7165..7332
                     /locus_tag="VKQ27_11170"
     CDS             7165..7332
                     /locus_tag="VKQ27_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89533.1"
                     /translation="MAEPEFGGVQLFPSPDSLTRLLMQADRVVETEFNALVRKIAAAL
                     ESRPSSCGARA"
     gene            complement(7435..8613)
                     /locus_tag="VKQ27_11175"
     CDS             complement(7435..8613)
                     /locus_tag="VKQ27_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
                     /protein_id="HLY89534.1"
                     /translation="MFWQKLHDAIVAMLHVERRFEPFFRSAVDAVLREPGAAVIQYLI
                     NRQRRDERLGRAEERELPDEAEHLQSIIDTMAEYMRRHWQPGNYQRAGNTKTHGVVRG
                     EVTIRDDLPAPFRHGVFAEPKTYRAWVRFSGPGPDWPRDIDDVGFVSCAIKLMGVPGP
                     KLLDDEKFTQDFLSICTPTFVTPDTRANAKLQSWILKGRPLFYFTDPRDSHLLDGLMQ
                     SWWNKTQSNPLGERYWSCVPYLLGDGQAMMYSVYPRSKVYRHIPRLPLTPPDDYLRDN
                     MVKTLAEQDVEFDIMLQLQTDPFRMPIENAAVRWPERLSPFVPAARLFIPRQKFDSPA
                     QLAFANNLSYNPWHCLPEHRPLGNQSRARRRMYYELSQFRQRVNATPHIEPTGDEVFV
                     "
     gene            8895..9329
                     /locus_tag="VKQ27_11180"
     CDS             8895..9329
                     /locus_tag="VKQ27_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89535.1"
                     /translation="MAYDVPQLLKMSQAELDALFTASQAGPIPEGQANGTAIIAPGTT
                     FSPRIADFISHFAWQGKVFDPKSSTLRNRIGPFGLNAIIARVYKAPSWLDNEECIVLD
                     YSETSLVAHWIRDEIRLIQPPGLYLGKVFWDKSRLIDFSLQF"
     gene            9345..>9574
                     /locus_tag="VKQ27_11185"
     CDS             9345..>9574
                     /locus_tag="VKQ27_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89536.1"
                     /translation="MTPQSNFMVLAPILAEREHDLRALLATMTAAPGMAEPNNSLFPF
                     ARFPRVHVARFVILDDATLDDLPPGDPLRHALL"
BASE COUNT         1945 a         3293 c         2723 g         1613 t
ORIGIN      
        1 cacccagcgc tgcgcccagg catttacccg cgccagacgc tccgaggata gcccaacctc
       61 ttccggtttc gcctgcattg ctcgctcctt cgcttcaggc cgttgcccag cgccgcgcgg
      121 tgcgggcgcg gctcagcaga aacaccgcaa tacaggcgtt gagcaggttc cacaacgcgc
      181 cattggcgaa cgctgcgtgg taggaaccag tggcgtcgaa gatcacgcca gagatccagc
      241 cgccaaacgc catcccgaac agggtcgcca tgataacgat accgaagcgc atgccggcct
      301 cgcgcgccgg catgtactcc cggacgatga tggcatagca gggtacgatg ccgccctgca
      361 cgagcccgaa aagcgcggat attgcataca acggatagag ggtgctgaaa ccgatataca
      421 gcaatagggc cagcatctgg ccgatcgagc cgatgagcag cgtgggcagc ccgccaagcc
      481 gatcggcgag gaagccggag ccgacacgac tgacaattcc taggccaagc atcagcgaca
      541 gcatttcagt accgcgggta atgccatagc cgagatcgcc gcagtaagcg acgatatgca
      601 cctgcggcat cgacatggcc acgcagcaac agaatcccgc cagcacgagc gtcgtctgta
      661 gcgcgcccgg cgataggccg agcgatgccg tggatacgct tgtcgtgacg gtgccgacgt
      721 gtaccggcgg cctgcgccgc agcgtcagtg ccagcgggat catggtaacg gcgcatagga
      781 tgccgatacc caacaacgtc tgccgcaggc cgatcgactg atcgtagtgc tgcacgagag
      841 gcggccaagc agtgcccgcc agatagttgc cagacgccgc aatggcgaca gcgatgcctc
      901 gccgccgagt gaaccagtgc gatatgtcgg ccaccatcgg gccgaatgtt gccgcaccac
      961 cgaacgcgac cagcactcca tgggcaagcg cgaactgcca caggctggtc gatgcagccg
     1021 ccgcgatata accagcgctg agagcggcgg cgccaatcag tgtcgaccac atgatgcccc
     1081 agcgatcggc catgcgcccc atcatcacgc cgcccaatgc gaagcccacc atcgcaagcg
     1141 tatacggcaa tgaggcggtg gcgcgatcga cgccaaattc cgtctgcacc gccggcagca
     1201 cgacaaccac ggaccacaga ctgatgcccc cgatggtgct cagcgccacg ctgatcgcga
     1261 gacgcagcca ggcatacgcg gattctggag ccgcttcggt cggcacagtt ggacggtcct
     1321 tcccattccc atgtcatcgc gaggcgcagc gatgaaatga caggcgctta tgtcaaatga
     1381 aggccgcctc gctcgccgcc ttggcctcgc gctgcagcca ctcgtaccac gccgtcatac
     1441 cctctcccgt gcgggcggac agccgcagaa ccgacatatc aggattgacc tcacgcgcgc
     1501 aggccaatgc acgctgaaca tcaaagtcca catgtggcag caggtcgacc ttgttcagca
     1561 gcatcagcga cgccgcacgg aacatgtgcg gatacttcaa tggcttgtcg tcgccctcgg
     1621 tgaccgagag cagcacgacc ttcgcgcgct cccccagatc gaacatcgcc ggacacacca
     1681 gattcccgac attctcgatc atcacgatgg accgcgtggg cggccgcaac tcgttcaggc
     1741 cgcgcgccac ctggtccgcc tccagatgac agccggttcc ggtgttcacc tgcacaacgg
     1801 acgccccggc tgcgcgaacg cgatcgctgt ccagcgtggt ggcctgatca ccttccagca
     1861 cgccgatatt ggtctgccca tccagatcac gaatggtccg ctccagcaac gtggtcttcc
     1921 ctgccccggg cgcgctgacc agattgatgg ccagaatctc ccgccccgcg aaccaggcac
     1981 gattgcgcgc cgccaatgca tcgttcttcg ccaggatgcg cgtgttcaac gcgatgaccg
     2041 tggtgcgatg ctcgtggtca tgatgatggc tatgccgcgt gccgtcggcg tggacgtgct
     2101 cgtgctccat ctctggctgg ccgcaaccgc aatgtccgca catcacacag cctcctcaac
     2161 ttccagggtg accacatgca gctcatcgcc attggtgcgt cggatgttga gccgcgcacc
     2221 ctccgccaaa gttccctgcg caaccacatc gaagcagaat gccagcgcat ccggcgaaac
     2281 gcaggacaac ttgccgactt ccaccgagat catatgcacg cgctggcccg atgccgcatt
     2341 gcaggcgata gaaacgatct cccgtgtcag cgacagctcg tgcatcagca gatccgaggc
     2401 agttggtcac ccaccagcat atcgacgatc cgatccccgc cgagcacggt gtgcatcacc
     2461 accagcccgg gccggcgctc cacaatctgc ccaatgcggc aagcgcccaa gccgagcgga
     2521 tgcccgcgca ttgccgccaa cgcacgcgcc gcctcgtcag gaggcaccac ggcgaccagc
     2581 cgtccctcat tcgcaagata cagcggatcc agcccgagaa tctcgcagaa cgccttcacc
     2641 ggctcgcgca gcggcaccgc cgtttcgctc agttcaatcc cacactgtgc ggcgtgcgcg
     2701 atctcattca gtaccgtcgc aacaccaccg cgcgtcgcat cacgcatgaa gcgcgtattc
     2761 ggcgcggcca ccagcagcgt ctcgaccagc ccatgcagcg cagcgcaatc gctatcgatc
     2821 ggcgcatcca gcgcaagatc gccgcgagcg gcaagaatgg cagcgccatg atcgcccaat
     2881 acaccgttca ccagcacgac atcgcccggt cgcgcgtact gcgcacccag cacgacgtca
     2941 ccgcgaatca ccccaattcc tgtcgtggtc aggaacagct tgtcgcaagc cccgcgctgc
     3001 acgaccttcg tgtcgccggt gacgatgtca acgccagcgt cacgtgccgc caaagccatc
     3061 gaccgcgcca tacgctccag gaggtcgaac ggcagcccct cctcgatgat cgcagcacac
     3121 gacaggtaca gtgggatcgc accacccacc gccaggtcat tgatcgtgcc gcacaccgcc
     3181 agtttgccga tatcgccgcc aggaaactcc agcggatcga ccacaaacga atccgtggtg
     3241 aacgccaacc gatcgccata ctgcgccagc gacgccagcg gtacgcgcgc ctgatcatcc
     3301 agcggcgcca atagcggatt atcgaacacc gacacgaaca cgacgtcgat caggtccttc
     3361 atcgccttgc cgccgccgcc gtgactgagc gtgacagtct tcatacggtt ttcgcggcat
     3421 agcgataata tgcagcgcac gcgccttccg aggacaccat cagcgccccc atgggcgtct
     3481 ccggtgtaca gtcggtacca aacaccctgc actgttgcgg cttcaaagcg cctttcagca
     3541 cctccccgca ctgacacgcc gccggatccg taacccgtac gcccggcacc acgaaccgcc
     3601 gctctgcatc gaaccgggca tacgcctcgc ggaaccgcac ccccgagtga tcgatcgaac
     3661 ccagcccgcg ccactcgaaa tgctcgcgcg gctcatacac cgcgtcgatc gcccgctgcg
     3721 ccgctgcgtt gccgccggat gccaccaccc gcgcatactg gttctcgatc tccgcgcggc
     3781 catcggcgat ttgccgcagc accatccaga ccgattgcaa caggtcgagc ggttcgaagc
     3841 cagagatcac cagcggcttg cgatactgcc gcgcgatgaa ttcgtatggc gcgctgccga
     3901 tcaccatgga cacatggccc gggccaacaa agccatcgat acgcagatcg gggctgtcga
     3961 gaatggtctt cagcgtcggc acaatggtga tgtgattgca gaacaccgag aagttgctga
     4021 taccgtcccg ctccgcctcc agcacggtaa gcgcggtgga cggcatggta gtctcgaagc
     4081 cgagcccgaa gaacaccacc tcccgctccg gattattccg ggcaatcgcc aatgcgtcgg
     4141 tgggcgaata caccatgcgg atatccgcgc cgttggcctt cgcctgcatc aggctgcctt
     4201 gcgaccccgg cacccgcatc gcatcgccga acgtcacgag gatcactcct ggctgcgtag
     4261 cgatcgcgac gcagtcatcg acgcgtccca tcggcagcac gcagaccggg caacccggcc
     4321 catgcacgag ttccacggca tccggcagca tcgcctcgat gccatagcgg aagatcgcat
     4381 gcgtgtggcc gccacagact tccataatct gcaacgcttt cgccggccgg atctcctccg
     4441 taacggcctc gatggcatgc aacagcgcgg cagccttgtc gcgatcgcgg aattcgtcga
     4501 cgtatttcat ggcgccatct cgcctagctc ggtcaacacg cccagcgtgg cttctgcctc
     4561 tgccgcatca atgcggctca ttgcgaaacc aacgtgcaga agcacccagt cgccgacaca
     4621 ggattccggc ggatgatgat catcgacgat gcagatgatg ttgacctgcc gccgtacccc
     4681 gccaacttca gcggttgcca gcatgcggtc cgcatcatcg atgcagacga tctgcgcggg
     4741 aatgccaaga cacatgggcg cctcctacgc catcagccga gcggcggcta tcgcagcctg
     4801 gcccaatgcc agcccaccat cgttcgtcgg cacagtactg tgtgacagca cacgataacc
     4861 tgcatcgcgc agttggctcc ctataagctc gagcagcacg gcattctgaa agcagccgcc
     4921 ggacagcgcc acggtatcga atccgtgcag ccgacgcgtc atcgccacga ccgccgccgc
     4981 aacaccttta tggaagcgcg ccgccatgac ctctggcggc acctgcgcct gcagatcatt
     5041 catgacagcc tgccacagcg gcgccggatc gatccagcca tccaccacca tcggatacgc
     5101 agcttcacca gtcagatcgc acggtcgcac cagcgcctca aggcgcgagg ccgcctcgcc
     5161 ctcatgtccc tgccgctcgc ggcagatacc aagtgctgct gccatcgcat cgaacagccg
     5221 gccgcaggat gacgccaacg gcgaattgat accggcccgg atcatcgcgt cgagcatcgc
     5281 gtggggccgt tgcagcaggc cagccgccgc aaggtgcgca tagaggttgc gccatggctc
     5341 ccgcaccgcc gccacgccac ctggcatcgc cactggcttg aacatcgcca gccgccgggc
     5401 ctgccgataa tgcgccaaca ggaactcgcc cccccagatt gtgccgtcat caccccagcc
     5461 cagcccgtcc agcacgatcc caagcaccgg cggagcatcg agcggccgcc cattctccgc
     5521 taggcacgcg gcgacatgcg cgtggtggtg ctgcacctcg atcaagctca gcccgcactc
     5581 gcgcgcgtat ttcgaagaca agtactcggg atgcgcatca gctgcgaccg tcgttggcgt
     5641 atggtcgaat agttccgcga agtgttcgat ggcgcctcga taggcgtcga aggttcgcgc
     5701 atcttcgagg tcgcctatgt gctgcgacag gaacgccttg ccgtcggtca gcaggcagaa
     5761 agtgttcttc tgctcagcac ccgcggcaag cagatcaggc gctccttcga accccgctgg
     5821 caacgctatc gccgccggag catagccgcg cgaacgccgc accaggcgcg caataccacc
     5881 aacgacacgg accacactgt catcgacccg cgtcacgatg acgcggtcat ggctgatcag
     5941 cacatcggct atgccgccaa gccgttcccg cgcctcgccg tcatcgataa tctgtggctc
     6001 gtcggagagg ttgccactcg tcatcacaac aggccgattg atatcctgca gaagcagcag
     6061 atgcagcggg gtggttggca gcatgacgcc cagggttttg acgccgggtg caaccgcatc
     6121 tggcaagcgt tcgccgcgaa cccgcatcaa cacaatcgga gcctcaggac tggaaagttg
     6181 gcgcaattcc tctgccccca ccgcgcaata acggtggact atctcggcat cgcgcgccat
     6241 caacgcgaac ggcttggtct cccggtgctt cgcgtcacgc aggcgcagca ccgaaccggc
     6301 attcgtggca tcacaggcaa gctggtagcc gccaatcccc tttaacgcga cgatgccgcc
     6361 cgactgcagc acctgcgctg cgatctcgac atcgaactgc agtcgaggcc cgcacgcggg
     6421 gcacgcaatc gcctcggcgt ggaaccggcg atcacccggc tcctcgtatt cctgccggca
     6481 agcagagcac agtccgaatg gcgccatcgt cgtagcagac cggtcatatg gaatctgacg
     6541 caaaatgctg aaccgtggac cgcactgcgt gcagttagcg aacgcgtaac cggcccggcg
     6601 ctgcccgcgg tccagcacct cgcgcgcaca ctccgcacaa accgcagcgt ccggggtaat
     6661 ctccgtccgg ggctcgcccc ccgcgctcgc aacgatgcgg aacccgggtg cgcagcttcc
     6721 ctcgatcgcc cggcagtcca ttcgctcgat gcgggccaac cgaggctgac cgtcgcgcaa
     6781 ccgctgcaac aggccgttga tcgccgcttc ctcgcccgca acgcggatca gcaccccacc
     6841 ggggtcattg cgcacgtcgc ccgacaggcc caactcgcgc gcaatccgcc acacggcggg
     6901 ccggaaaccg acgccttgaa cgcgcccgcg gacgcggatt tcggactgct gcatccccat
     6961 cgatccaatg ccgcccggca acgtgctggc caaatggtaa acgcagtatg aaaaacgcgc
     7021 cacaacacgt tgaatatgac cggatgaaat gtttcaacac gcccgtgaca tggttctgac
     7081 acacgccgct accacatact gctctgccgg cgcggaaggg acagtcaaat gtacactgca
     7141 tgcgagacct gcggtcgtcc cctgatggct gagccagaat ttggtggcgt ccagctgttt
     7201 ccgtcgcctg actcgttgac ccgcctgctc atgcaggcgg atcgggtcgt cgaaacggag
     7261 ttcaatgcat tggtacgcaa gattgccgcg gcattggaaa gccgccccag cagttgcggg
     7321 gcgcgcgcct gaccactggc caggggaggg tcgaagcggc cacgaatatc ccctgcgggc
     7381 agcaacgacc gtcgctgagc tgcagttcag ccgctccatc gccatcctgt cgtcctacac
     7441 gaaaacttca tcgccagtgg gttcgatatg aggggttgcg ttcacccgct gccggaactg
     7501 ggacagttca taatacattc gccgccgggc gcggctctga ttccccagag gacgatgctc
     7561 gggaagacaa tgccaagggt tgtaggacag gttgttcgca aacgcgagtt gtgctggcga
     7621 atcgaatttc tgtcgaggga tgaatagtcg ggctgccggc acgaacggag acagacgctc
     7681 cggccaacgg accgcggcat tctcaattgg catccggaat gggtctgtct gcaactgcag
     7741 catgatgtcg aactccacat cctgctcggc cagcgtcttc accatgttgt cgcgcagata
     7801 atcgtctggt ggtgtcagcg gcagccgcgg tatgtggcga tacactttcg acctcggata
     7861 gaccgaatac atcatcgcct gtccatcgcc caacaggtac ggcacacagc tccagtaccg
     7921 ctcgcccagc gggttgctct gcgtcttgtt ccaccacgac tgcataaggc cgtcgagcaa
     7981 gtgcgaatcc cgcggatcgg tgaaatagaa gagtggccgc cccttcagga tccacgactg
     8041 cagcttggcg ttcgcacggg tgtcgggtgt gacgaacgtc ggcgtgcaga tcgacagaaa
     8101 atcctgggta aacttttcgt catcgagcag cttcgggccc ggaacaccca tcagcttgat
     8161 cgcgcagctc acgaaaccca cgtcgtcgat atcgcgcggc cagtcgggac ccggaccgga
     8221 gaagcgcacc caggcacgat aggtctttgg ctcggcgaac acgccgtgcc ggaagggtgc
     8281 tggcaagtca tcgcgtatcg tcacttcgcc acggaccacg ccgtgcgtct tggtattccc
     8341 tgcgcgttga tagttccccg gttgccaatg ccggcgcata tactcggcca tcgtatcgat
     8401 gattgactgg agatgctccg cttcatccgg cagttcgcgc tcttcggccc tgccaagcct
     8461 ttcgtcccgc cgttggcggt tgatcagata ctgaatgacc gccgcgcctg gttcgcgcag
     8521 cacggcatcc accgccgaac ggaagaaggg ctcgaaccgg cgctcgacat ggagcattgc
     8581 aactatggcg tcgtgcagtt tctgccagaa catcggatcg acgctccccc gcggctatgt
     8641 gcctcttcct tgtatatatc gatgttgccc gagtgcagca aaatctgttg cataatggat
     8701 aaaatctatg ctgtggtcac gccatggcag cgcgcgcccg cagcggccgc actgcgcgac
     8761 tctgcggact atctgacgtc acaaacgtcg aatggcgagc tgatctcgcc gatgaatgac
     8821 aggttcggca ctatgccaga aagcatcgtt cgacagcgcg gccgcggcaa aacaacggaa
     8881 cccaggggga catcatggca tacgacgtac cgcaactctt gaaaatgtcg caggctgaac
     8941 tggatgcact gttcaccgcc agccaggccg gtccaatccc tgagggtcag gccaacggca
     9001 cggcaatcat cgcaccgggc accacgttca gcccgcggat cgcggacttc atcagtcatt
     9061 ttgcctggca aggcaaagta ttcgatccga aatcgtccac gctgcgcaac cggatcggcc
     9121 cgttcggact gaacgcgatc atcgcccgtg tctacaaggc accgagttgg ctggacaacg
     9181 aggaatgcat cgtactcgac tattcggaga catcactggt cgcgcactgg atccgcgatg
     9241 agatccgcct tatccagccg ccggggctct atcttggcaa agtattctgg gacaagagcc
     9301 ggctgatcga tttctcgctg cagttctgaa ctgccagccg cggcatgacg ccacagtcta
     9361 acttcatggt gttggcgcct atcctcgcgg agcgcgaaca cgatctgcgg gctttgctgg
     9421 cgacgatgac cgcggcgccc ggcatggctg agccgaataa ctcgctgttt ccgttcgctc
     9481 ggtttccgcg tgtgcacgtc gcgcgattcg tcattctgga cgacgctacg ttggacgatc
     9541 tgccgccagg cgatccattg cgccacgcac tgct
//
LOCUS       DATGEM010000353        11066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_129120, whole genome shotgun sequence.
ACCESSION   DATGEM010000353 DATGEM010000000
VERSION     DATGEM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11066
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129120"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VKQ27_11190"
     CDS             <1..663
                     /locus_tag="VKQ27_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89537.1"
                     /translation="QVRDLHLTVPSAAGPVNILRGIDLDIGAGEAVGIVGPSGSGKTS
                     LLMVLAGLERATSGSILLAGRLVTGLDEDALAGLRREAIGIVFQAFHLIPSMTALENV
                     AVPMELAGARDALPRAKDALAAVGLAHRLTHLPGQLSGGEQQRVALARAFAPRPRLLL
                     ADEPTGNLDQATGEAVMELLFQLRVETGATLMLITHDPSLAARCSRCVHMADGRVAEL
                     VG"
     gene            663..3167
                     /locus_tag="VKQ27_11195"
     CDS             663..3167
                     /locus_tag="VKQ27_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLY89538.1"
                     /translation="MLALRLARRDLRGGTRGLWIVLLCLALGVAVIAAVGSLRAATDQ
                     GLVTDGKRILGGDLEVESGTQPPPDALRDWLAARGGRISQVVQMRSMLVAPVTGERQL
                     VELKAVDGAWPLVGEAQIGHAAASAGRIAPLLANHGLIAEQVVVDRLNVRIGDTVRLG
                     NTSFILRAVLGSEPDRVAAPLLLGPRVLISAEALPSTGLVVPGSMVQYAIRTTVPDPA
                     IVEQQLKAGFPSQGWRIRDVHDAAPGVARFIDQTSLFMTLVGLTSLLVGGIGVANGVR
                     AWLDARAGTIATLRCLGAPSRLVFTACLLQVLALALTGIAIGLVLGAVTPIALASWLK
                     DVLPVPPVLGIYTRPLVLAAAYGLLTALAFSLWPLGRAARIPGAALFRDAFLPGRVLP
                     PAAIIAANAAIVAGLITLTIVTSTDRQFALWFCAAAVGTLLLFRLGGSFVMLAARAAN
                     KFGSAATRLGVASLHRPGAATPLMLVSVGLGLSTLAAVALIQGNVQREILDQLPANAP
                     SFFFVDIQNTELPRFESLVRAQPGVEDLEQVPSLRARIVAVNGVPADKVRATPNTAWA
                     LRGDRGLTYAATPPPGTHIVAGHWWPADYDGPPLVSFDANLAQGWGVHLGDTIRVNVL
                     GRDIDLKVGSLRDIAWQSLSLNFTLVASPGLLSHAPHTHIATVRVADADQGALLRAIT
                     DALPNVTGIRVQDVLAAIAALLDQVAAALTATGSLTLIAGALVLVGAVAAGRRRRIRE
                     AIILKALGASRRQIRTVWLVEFGSLGLAAGLIAALVGTLASFGVVHYLMHFEWTFLPS
                     RLAATLIASLAMMLVFGYAGTATALRAKPAPMLRNE"
     gene            3288..4058
                     /locus_tag="VKQ27_11200"
     CDS             3288..4058
                     /locus_tag="VKQ27_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLY89539.1"
                     /translation="MAFSPDYRTYSRASAPTTAAVLDAGLRAYMLRVYNWMSSGLVLT
                     GIIAYAIANTSLIDAFYPLVQTPFGMVRHASALAMISIFAPLAFVLVLSLGVNRLSKS
                     AAQTLYWVFCAAMGASLTNIFLIYTSESIVRVFFITAATFAAMSIYGYTTKTDLSRFG
                     SFLMMGLFGIIIAGLVNIFLHSSALQFAISIIGVVVFTGLTAYDTQRIKSSYVQYAYA
                     EGPDAAAKRSVYDALNLYLNFINLFMLLLQLLGNRNSN"
     gene            4221..4964
                     /locus_tag="VKQ27_11205"
     CDS             4221..4964
                     /locus_tag="VKQ27_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89540.1"
                     /translation="MATIVTAGGYRYIPHAFQYSGGVEALPGHRIERVEFSRPIPLAA
                     GFTWIEAYLGKLGVPLTGFCACELRSPEQFTDQGFIDFNRHYIGTLLRWGVMKNAEDN
                     PVARSNVIPLLDKPTEPSFHAFCFARPANGASGSFVIAGSGEASDEPVAYRERTIRYG
                     ETSPDAMREKALFVVGRMDQRMAVLAKTWVDTTATQIYSVHPFPAEDIVRCGAAWHGM
                     LWHYTRPPVEGLEFEMDCRSVPVEHHVIP"
     gene            5037..5660
                     /locus_tag="VKQ27_11210"
                     /pseudo
     CDS             5037..5660
                     /locus_tag="VKQ27_11210"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            5717..6529
                     /locus_tag="VKQ27_11215"
     CDS             5717..6529
                     /locus_tag="VKQ27_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HLY89541.1"
                     /translation="MLGHDITWIDWLIAAAPFSIVLSICLYYIAMVMLPPESSEVPGG
                     EAAVRRALADIGPMTGKEKRLLAISLILLCFWATEQILHRFDSATTTTVAVAILFLPG
                     IGVMSWNEVQPRIPWGTVVLFGIGISLGTVLLQTKAAIWLANLIVTGFGLNDLGALAI
                     LSVLSLFLIVIHLGFASATALASSLIPIIIAVLQKVQTPGINVIGMTMILQFVVSFGF
                     ILPVNSPQGMVAYGTDSFAVRDFIRVGLVLTVVAYLLTLVFGATYWRWLGYA"
     gene            complement(6526..7485)
                     /locus_tag="VKQ27_11220"
     CDS             complement(6526..7485)
                     /locus_tag="VKQ27_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLY89542.1"
                     /translation="MKASAGTAAKGRGASAPRPAAPRRTASTKPAKAAHAAAVKKIVA
                     APAKVAKAASAKTETPSAAPKPATAAKVIAAKPGPAKQEAKAHAVPTNAAPLPAPLAA
                     KPAPKPTAAPLQAVQEKAPAPALTPTAAAPAAVPVQSPGRPMMPDRPPPRPMAKAEIF
                     VEGDHVVYPTHGVGKVERIATEEIAGHKLELIHITFEENRMTLRVPVAKARTAGLRKL
                     ATRKLFDEALSVLKGRARIKRTMWSRRAQEYEAKINSGDPLAIAEVVRDLHRNAGQPD
                     QSFSERQIYEAAMDRLAAELAALDKTDKLTAATKLTNFLKTVS"
     gene            complement(7737..8069)
                     /gene="fdxA"
                     /locus_tag="VKQ27_11225"
     CDS             complement(7737..8069)
                     /gene="fdxA"
                     /locus_tag="VKQ27_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HLY89543.1"
                     /translation="MTYVVTETCIRCKYMDCVEVCPVDCFYVGANMLVIHPDECIDCG
                     VCEPECPVEAIVPDSDDKAGSWLEQNRTYAGIWPNITRKGEPPADADDWKDKPDKGKL
                     FDPEPGKP"
     gene            complement(8122..8358)
                     /locus_tag="VKQ27_11230"
     CDS             complement(8122..8358)
                     /locus_tag="VKQ27_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HLY89544.1"
                     /translation="MRARSDCAAMVAAGSIRINRQATDKAHARLRVGDVLTVPVHRSV
                     RVVRVVSLAARRGPASEARLLYEEILETEPGPCA"
     gene            complement(8526..11027)
                     /locus_tag="VKQ27_11235"
     CDS             complement(8526..11027)
                     /locus_tag="VKQ27_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLY89545.1"
                     /translation="MPGLDSGFAPRVKAVLGPTNTGKTHLAIERMLGHASGIIGFPLR
                     LLARENYDRMVAHKGERHVALITGEEKIIPPEARWFSCTVEAMPLDRDAEFLAVDEIQ
                     LCADPDRGHVFTDRLLHARGMVETMFLGAETIRPLLQRLVPQASVETRPRLSQLNHVG
                     QTKLVRLPPRSAVVAFSAGEVYAIAELIRRRRGGCAVVMGRLSPRTRNAQVALYQDKE
                     VDFLVATDAIGMGLNMDVDHVAFASLGKFDGHRPRRLAPAEVAQIAGRAGRGMRDGTF
                     GTTGQCPPLGDEVAAAVEAHSFDALDQLCWRNSDLDFSHVDALLDTLMVPPPRPGLVK
                     GNDAADFETLAALARDPDIRRMAEGRRMVGKLWEACQIPDFRKLADDTHTRLCARVFG
                     HIAKDGRLPTDWLASQIGMLARADGDIDTLMQRLAGVRVWSYVAARADWVQDSPHWQG
                     RAREVEDLLSDALHERLTSRFVDRRAAHLMRRLEAGEDQELLSAVTRRGEVIVEGHPV
                     GRIGGFRFFPDPAAEGDAKKLVLRAARRALREEMPRRVSAVEAAPDAALTLGAEIIWD
                     GAPIARLRRGISALRPRVEVSGSEFLDGAQRERMRLRLQRFVDDRIAAELAPLHKAVE
                     KAAGQPGFRGMLHRLTEALGMIPGDEGDGLPPTSRGVLKAIGVKAGRFGLFLPALLKP
                     RAAGMRALLWGLQHGVAAPELPSPDQVSLPARPDWPVGFADAMGWLEAGPVLLRIDIA
                     ERVAAELAWSARRGATAMPAGLASRFSLKTELLPVVLRRLGFRIVPAAGLSANEFGPP
                     APAMILPLRRRRTTVEPQVIVQAHGPFAVLAALKR"
BASE COUNT         1730 a         3821 c         3451 g         2064 t
ORIGIN      
        1 caggtcaggg acctgcacct gaccgtgccg tccgctgccg ggccggtcaa catcctccga
       61 gggattgacc tggatatcgg tgcgggcgag gcggtcggca tcgtcggtcc ctccggctcc
      121 ggcaagacca gcctgctgat ggtgctggcg ggactggagc gcgccacgtc cggcagcatc
      181 ctgctggctg gccgcctggt taccgggctg gacgaggacg cgctcgctgg actgcgacgc
      241 gaggcaatcg gcatcgtgtt ccaggccttc caccttatcc ccagcatgac cgcattggag
      301 aacgtcgccg tgcccatgga gttggctggc gcacgagacg cgctgccgcg tgcgaaggat
      361 gcgctggcgg cggtgggcct tgcgcatcgc ctgacccatc tgcctggtca gctttccggt
      421 ggtgaacagc agcgggttgc gctcgcccgc gcgttcgccc cgcgcccgcg cctgctgctg
      481 gccgatgaac ccaccggcaa tctggaccag gcgacgggtg aagcggtgat ggagctgttg
      541 ttccaactgc gtgtcgagac tggcgcgacc ctgatgctga tcacgcacga cccaagcctg
      601 gcggcgcggt gttcgcgctg cgtccacatg gcggatggcc gggtggcgga gctggttggc
      661 tgatgctggc gctgcggttg gcgcgacgtg acctgcgagg cggaacgcgc ggcctgtgga
      721 tcgtgctgct gtgcctggcg ctcggcgtcg cggtcatcgc cgcggtggga tcgctgcggg
      781 cggcgaccga tcagggactg gtgaccgacg gaaagcggat tctcggtggc gatctggagg
      841 tggagagcgg cactcaacca ccacccgatg ccttgcggga ctggctcgcg gcgcgcggtg
      901 gcaggatctc gcaagtagtg cagatgcggt cgatgctggt ggccccggtt accggcgagc
      961 gccagctggt ggagctgaag gcggtggatg gcgcctggcc gctggtgggc gaggcgcaaa
     1021 tcggccacgc ggccgcatcc gccggccgaa tcgcccctct cttggccaac cacggcctga
     1081 tcgccgagca ggtcgtggtc gaccgcctca atgtgcgcat tggcgacaca gttcgcctgg
     1141 gcaacaccag cttcatactc cgtgctgtgc tgggctcgga accggaccgt gtcgccgcgc
     1201 cgctgctgct tggtccgcgc gtgctgatct cggctgaggc cctaccctca acaggcctcg
     1261 tcgtccccgg ctccatggtg cagtatgcga tccggaccac cgtcccggat cctgccatcg
     1321 tcgagcagca attgaaggcc gggttcccca gccagggctg gcgcatccgc gatgttcacg
     1381 acgctgcccc aggggtggcc cgcttcatcg atcagaccag cctgttcatg accctggtgg
     1441 ggcttacatc ccttctggtc ggcgggatcg gcgttgccaa tggggtccgc gcatggctcg
     1501 atgcccgggc cgggaccatc gccaccctcc gttgcctcgg cgccccctcg cgccttgtgt
     1561 tcactgcgtg cctgttgcag gtcctggccc tagcgctgac gggcattgcg atcgggctcg
     1621 tgctgggagc cgtcaccccg atcgctcttg ccagctggct gaaggacgtc ctgccagttc
     1681 cccctgttct cggaatttac acccggccgc tggtcctcgc cgctgcctac gggctgctga
     1741 ccgcactcgc gttctcgctc tggccgctcg gccgggccgc ccgtatccct ggcgctgcgc
     1801 tcttccggga cgcattcctc ccggggcgcg ttctcccgcc cgccgcgata atcgctgcca
     1861 atgccgccat cgtcgccggc ctgatcactc taacgatcgt cacatctacc gatcgccagt
     1921 ttgccctgtg gttctgcgcc gcggcagtcg gcacgttgct gttgttccgc ctgggcggct
     1981 cgttcgtcat gctcgcggcc cgcgccgcca acaagtttgg cagcgccgcc acccgcctgg
     2041 gcgtcgccag cctccaccgc cccggtgcgg ccacaccgct gatgctggtc tcagtcggtt
     2101 taggcctctc taccctggcc gccgtagcgc tcatccaggg taacgttcag cgcgagatcc
     2161 tcgaccagct tcccgccaac gcccccagct ttttcttcgt ggacatccag aatacggagc
     2221 tcccacgttt tgagtccctg gttcgcgccc agcccggcgt ggaggacctc gaacaggtgc
     2281 ccagccttcg cgcccgcata gtcgcggtga acggcgtgcc ggcagacaaa gtgcgcgcca
     2341 cgcccaatac cgcctgggca ctgcgcggcg atcgggggct cacttacgcc gccactccac
     2401 ctcccggcac ccacatcgtc gccggccatt ggtggccggc cgactacgac ggacctccgc
     2461 tggtctcgtt cgacgccaat cttgcccaag gctggggcgt ccatctcggc gacacgattc
     2521 gggtcaacgt actcggccgg gacatcgacc tgaaagtcgg cagcctgcgc gatatcgcct
     2581 ggcaaagcct ctccctgaac ttcaccctcg tcgccagtcc cggtctgctg tcgcatgccc
     2641 cccataccca catcgccact gtccgcgtcg ccgacgccga ccagggtgct ctgctgcgtg
     2701 ccatcactga cgccctgccc aacgttacgg gcatccgcgt ccaggatgtg ctggctgcca
     2761 tcgcggcgct gctcgaccaa gtggctgccg cccttactgc gaccggctcc ctcactctga
     2821 tcgccggtgc cctggtcctg gtgggtgcgg tagccgccgg ccggcgccga cggatccgcg
     2881 aagcaatcat cctgaaggca ctgggcgcca gccgccgcca gatccgcacc gtatggctgg
     2941 tggagtttgg cagcctgggc ctcgccgccg gactgattgc agcgctggtg ggtaccctgg
     3001 caagtttcgg ggtggtccac tatctgatgc acttcgaatg gacgttcctg ccaagccgcc
     3061 tggcggcgac gctgatagca tctctcgcca tgatgctggt gttcggctat gctggcactg
     3121 cgaccgcgct gcgggccaag ccggccccga tgctgcggaa cgagtgaagg ccggcgtacc
     3181 gaggatgggt taccgagctt tcattaccat aatggcttga accgctccgg cccctctaca
     3241 aaatgagagg ccacggacaa cctgcccgaa aggcaaagag gacacacatg gccttcagtc
     3301 ccgactaccg gacctactcg agagcatcgg cccccacgac cgctgcggtc ctggacgccg
     3361 ggctccgcgc atacatgctg cgggtctaca attggatgag ttccggtctc gtgctgaccg
     3421 gtatcatcgc ctatgcaatc gccaacacca gcctgatcga tgcattctat ccgctggtgc
     3481 agacgccgtt cggcatggtg cgccacgcct cggccctggc gatgatttcg atctttgcgc
     3541 cgctggcctt cgtgctggtg ctgagcctcg gcgtcaatcg gctgtcgaaa tcggccgcac
     3601 agacactcta ttgggtgttc tgcgccgcga tgggcgccag cctcaccaat attttcctga
     3661 tctacaccag cgagtcgatc gtgcgggtgt tcttcatcac agcggccacc ttcgcggcga
     3721 tgagcatcta cggctacacg acgaagacgg acctgagtcg cttcggcagt ttcttaatga
     3781 tgggcctgtt cggcatcatc attgccgggc tggtcaacat tttcctgcac agctcggcgc
     3841 tgcagttcgc gatcagcatc atcggcgtcg tcgtgttcac cggccttacg gcctacgaca
     3901 cgcagcggat caagtcctcc tacgtgcagt atgcctacgc tgagggcccg gacgcagccg
     3961 ccaagcgcag cgtctatgat gcgctgaatc tttacctgaa cttcatcaac ctgttcatgt
     4021 tgctgctgca gttgcttggc aaccgcaaca gcaactgacg tcaggccagt cttctgtgcg
     4081 aagggcctcg gggcaacccg aggccctttg cttttaccct cttcggttcc cgacacggcc
     4141 gggcgtgact cggcgaccgt ttgcgtcccc actgttccct gccttgaata ccgtccgcgc
     4201 agcaacacag ggcaacctca atggcaacga tagtaacggc cggtggctac cgctacatcc
     4261 cgcatgcctt ccaatactcg ggcggcgtag aagcccttcc gggccatcgg atcgagcgcg
     4321 ttgaattctc gcggcccatc ccgctcgccg cgggcttcac ctggatcgaa gcctatctcg
     4381 gcaaacttgg cgtcccactg accggcttct gcgcctgcga gcttcgttct cccgagcagt
     4441 tcaccgacca gggcttcatc gacttcaacc gccactacat tggcacgctg ctccgctggg
     4501 gcgtgatgaa gaacgctgaa gacaacccgg tggcacgcag caacgtcatc ccgctgctgg
     4561 acaagcccac cgaacccagc ttccatgcct tctgctttgc gcgtcctgcc aacggcgcct
     4621 ccggctcctt cgtcatcgcc ggcagcggtg aggcgagtga cgaaccggtt gcctaccgcg
     4681 aacgcacaat ccgctatggc gaaaccagcc ccgatgccat gcgtgagaag gcgttgtttg
     4741 tcgtcggacg tatggaccag cgtatggccg tgcttgccaa gacctgggtg gacaccaccg
     4801 ctacacagat ctacagcgtg catcccttcc cggccgagga catcgtccgt tgcggcgccg
     4861 cttggcatgg catgctctgg cactatacgc gcccgcccgt cgaaggcctg gaattcgaga
     4921 tggactgccg cagcgtgccc gtggagcacc acgtcatccc gtagccgttg acccagatca
     4981 acgcatcata aggaagttcg tgcgacaggc gcgaagccag ggaagaggcc gcgcccatgt
     5041 caaccaccac cctgatcgcc aagcccacga ctccgctgcc gaaaatatgg tcgctggcgc
     5101 tcgccgtcac cgtcctgctg gtcatcctgt tcgcgccggc actggctggc ctgccgatcg
     5161 caggccaacg aatgctggcg gtgttcggct ttgcgatcgt ggtctgggtg accgaggcgc
     5221 tggactacgc cgtaagcgcc gtggtgatcg ctggcctgat ggccgtgttg cttggcatct
     5281 cgcccgaccc ggccaagcct gccgctctgc tgggcacgac ccaaggtctg accatggcgg
     5341 tcagcggctt cggcaacacg gcgctgatcc tggtcgctgc tgctttgttc ctcgctgccg
     5401 cgatgtcggt aactgggctc gaccgccgga tcgccctggt cattctctcg catgtcggca
     5461 cgtctgcggc acgtatcgtc attggcggca ttgccatcgc cgtcgcgctg agcttcctgg
     5521 tgccgagtgc cacggcgcgc gccgcggtgg tcgtgcccat catgctcggc gtcgtggttg
     5581 cgttcggcgc cgacaaacgg agccggtttg cagcgcttgt catgctcacc acggtgcagg
     5641 cggtcagcgt ctggaatgtc ggggtgaaga cggggccgcc gcgcagaaca tggttgccat
     5701 cggcttcatc cagcgcatgc tcggccacga catcacctgg atcgactggc tcatcgccgc
     5761 ggcaccgttc tccatcgtgc tgtcgatctg cctctactac atcgccatgg tcatgctccc
     5821 gcccgaaagc agcgaagtgc caggtggcga ggcggcggtg cggcgagccc tcgcagacat
     5881 cgggccgatg acgggcaagg agaagcggct gctggccata tcgctgatcc tgctctgctt
     5941 ttgggcgacg gagcagattc tccatcggtt cgacagtgcc accacgacca cggtggccgt
     6001 cgcgatccta ttcctgccag gtatcggcgt gatgagctgg aacgaggtgc agccgcgtat
     6061 cccgtggggc acggtcgtgc tgttcggcat cgggataagc ctcggcactg tcctgcttca
     6121 gaccaaggcg gcgatctggc tggccaacct gatcgtcacc ggtttcggcc tgaatgacct
     6181 tggtgctttg gcaattctct ccgtgctgtc gctgttcctg atcgtgatcc atcttggctt
     6241 tgccagtgcg actgcgctcg cgtcttcgct gatcccgatc atcatcgccg tcctgcagaa
     6301 ggtgcaaaca ccagggatca acgtcatcgg catgacgatg atcctgcagt tcgtggtcag
     6361 tttcggcttc atcctgccgg tgaactcgcc gcagggcatg gtggcctatg gcaccgactc
     6421 gttcgctgtg cgcgacttca tccgcgtcgg tctcgtgctg accgtcgtcg cctatctgct
     6481 gactctagta tttggcgcca cctattggag gtggctcggt tatgcctagc tcaccgtctt
     6541 caagaaattg gtcaacttgg tcgccgcagt aagcttgtcg gtcttgtcga gcgccgccag
     6601 ctcggccgca agtcgatcca tcgccgcctc gtagatctgc cgttccgaaa aactctggtc
     6661 gggttggccg gcgttgcggt gcaggtcgcg caccacctca gcgatcgcca gcgggtcgcc
     6721 ggagttgatt ttcgcttcat actcctgcgc ccggcgcgac cacatggtcc gcttgatccg
     6781 cgcgcggccc ttcagtaccg acagcgcctc atcgaacagc ttgcgagtgg cgagcttccg
     6841 cagaccggca gtccgtgcct tggcgaccgg cacacgcaag gtcatgcggt tctcctcgaa
     6901 cgtgatgtgg atcagttcga gcttgtggcc ggcgatctct tcggtggcga tgcgttccac
     6961 cttgccgacg ccatgcgtcg ggtaaacgac gtggtcgcct tcaacgaaga tctccgcctt
     7021 ggccatcggc cggggcggcg gccggtccgg catcatcggc cggccgggcg actgtacagg
     7081 gactgctgcc ggggcggcgg ctgtgggtgt cagagcagga gccggggcct tctcctgtac
     7141 ggcctgcaac ggcgcggcgg tcggtttcgg ggcgggcttg gccgcgaggg gagcaggcag
     7201 gggggcggcg tttgtcggca cggcgtgggc ctttgcctcc tgcttggcgg gaccaggctt
     7261 cgcagcaata accttggcag cggtcgccgg cttcggagcg gccgacggag tttccgtctt
     7321 tgccgaagcg gccttcgcca ccttggccgg cgctgcgacg atcttcttga ctgccgcggc
     7381 gtgcgctgcc ttggccggct tggtggacgc cgtccgacgt ggggccgccg gacgcggagc
     7441 cgatgctccc cgacctttcg ctgcggttcc cgcagatgcc ttcatgcctg acacctcttt
     7501 aatcttcgtc cttcaaacgt tgcgatccac ccacggttcg cgcggccatg tcctcggcca
     7561 gcgcgcaccg ctccgatggt tcgcctcctg ggggtagcgg gagcagtccg cgcttttggg
     7621 cagccgcctc cactgacacg cccctatata gcatcacagc ggtgttacgt catgggggca
     7681 cgcggaacga acgacgcaaa gcgtcgcccg gcggtcacac ttcggcttga ggcggatcag
     7741 ggcttgccgg gctcggggtc gaacagcttg cccttgtcgg gcttgtcctt ccaatcgtcg
     7801 gcatccgcag gcggttctcc ctttcgggtg atattcggcc agataccagc ataggtgcgg
     7861 ttctgctcca gccaggaacc cgccttgtcg tcgctgtccg gcacgatcgc ctcaacgggg
     7921 cattccggct cgcagacccc gcaatcaatg cattcgtccg gatggatcac cagcatattg
     7981 gcgccaacat agaagcagtc gacagggcat acctcgacgc agtccatata cttgcagcga
     8041 atgcaggtct cggtgaccac ataggtcata ggtcagtccc cggcgctctt gtcgacggcg
     8101 gcgatatgcc gatgattgtg ctcaggcgca aggtccgggc tcggtttcaa ggatttcctc
     8161 gtatagcagg cgagcctcgg aagccgggcc tcgcctggcc gccagggaca ccacacgcac
     8221 aacgcgcacc gagcggtgta ccggcaccgt cagcacgtcg ccgacgcgca gccttgcgtg
     8281 ggccttgtcc gtcgcctggc ggttgatgcg gatcgatccc gcggcgacca tcgccgcgca
     8341 atcgcttcgg gcacgcatga accgcgcaca ccagagccac ttgtccaggc gctgccagtc
     8401 tcggtcttcc tgctccgcag gcacttatcg gaaccaaaat tgggcaccgg cggaggcaga
     8461 tggggttcgg ccatgtattg atcggcgtcc atctgcatcc atttgcgatt ccaattcctt
     8521 cgttgctatc gcttcagtgc cgccagcacg gcgaagggcc catgcgcctg cacaataacc
     8581 tgcggttcga cggtggtgcg cctgcggcgc aatggcagga tcatcgctgg tgccggcggt
     8641 ccgaactcgt tggccgacag cccggccgcc ggcacgatac gaaatccaag acgccgcagc
     8701 acgaccggca gcagctccgt cttcagtgaa aaccgcgaag ccaaccctgc cggcatggcc
     8761 gtggcaccgc gccgtgctga ccaggccaat tcggcagcca cgcgttcggc gatgtcaatg
     8821 cgcagcaata ctggtccggc ctccaaccac cccatggcgt cagcaaaccc cacaggccaa
     8881 tccggccgag ccggcagcga cacctggtct ggcgacggca gctcgggcgc cgcaacgccg
     8941 tgctgcagcc cccacaggag cgcccgcatg ccagcggccc gaggcttcaa aagcgccggc
     9001 agaaacaggc caaaccgccc cgccttcacg ccgatcgcct tgagtacccc acgggaggtg
     9061 ggcggcagtc catctccctc atcgcccggg atcatcccga gtgcctcggt cagccgatgc
     9121 agcatgcctc ggaaaccggg ctgaccagcc gccttttcca ccgccttgtg cagcggggcc
     9181 agctcggccg cgatccggtc gtcaacgaaa cgctgcaggc gaagccgcat ccgctcgcgc
     9241 tgcgcgccgt ccaggaactc gctgccggat acctcgaccc gcggccgcaa tgcgctgatg
     9301 ccgcgccgca atcgggctat cggcgcgcca tcccagatga tctccgcacc cagagtgagc
     9361 gccgcatccg gcgccgcttc gacggcggac acccggcgcg gcatctcctc gcgtagtgcg
     9421 cggcgcgcgg cgcgcaacac cagcttcttc gcatcccctt ccgctgccgg atcagggaag
     9481 aagcggaaac cgccaatccg tccaacggga tgcccttcca cgatgacctc gccgcggcgg
     9541 gtcaccgccg acagcagctc ctggtcctcg cctgcttcca atcgtcgcat caggtgcgca
     9601 gcgcggcgat cgacgaaccg gcttgtcagc cgctcgtgca gcgcgtcaga cagcaggtcc
     9661 tcgacctcgc gcgcgcgtcc ctgccagtgc gggctgtcct gcacccagtc cgcccgcgcc
     9721 gcgacgtagg accacacccg aacaccggcc aggcgctgca tcagcgtgtc gatgtcgcca
     9781 tccgcccgcg cgagcatacc aatctggctg gccagccaat cggtgggcaa tcgtccatcc
     9841 ttggcgatgt gcccgaacac ccgcgcgcac agccgcgtgt gcgtgtcgtc cgcgagcttg
     9901 cggaagtctg gaatctggca cgcctcccac agtttcccca ccatccggcg cccctcggcc
     9961 atccgccgga tgtcaggatc gcgtgcgaga gccgcgagcg tctcgaaatc ggctgcgtcg
    10021 ttccccttca ccaggcccgg cctaggcggc ggcaccatca gcgtgtcaag cagcgcatcg
    10081 acatggctga agtctaggtc gctgttgcgc caacagagct ggtcgagcgc atcgaaactg
    10141 tgcgcctcga cggccgctgc gacctcatcg ccaagtggcg ggcactggcc cgtcgtcccg
    10201 aacgtcccgt cccgcatccc ccgcccggcg cggccggcga tctgcgccac ctcggcgggt
    10261 gccagccggc gcggccgatg cccgtcgaac tttcccaggc tggcgaaggc gacatggtcc
    10321 acgtccatgt tcagacccat gccgatggcg tcggtagcca ccaggaaatc gacctctttg
    10381 tcctgataca acgccacctg ggcgttgcgg gtgcgcggcg acagccgccc catcaccacc
    10441 gcacagccgc cccgtcgacg ccggatcaac tcggcgatcg cgtaaacctc gccggcgctg
    10501 aacgcgacca ctgccgaacg cggcggcaat cgcaccagct tggtctgtcc gacgtggttg
    10561 agctgcgaca accgcggccg ggtctccacg cttgcctgtg gcaccagccg ctgcagcagc
    10621 ggccggatcg tctcggcgcc gaggaacata gtctccacca tgccccgcgc gtgcagcaac
    10681 cgatcggtga acacgtgccc gcgatcggga tcagcgcaga gctggatctc atccaccgcc
    10741 aggaattcgg catcccgatc cagcggcatc gcctccacag tgcaggagaa ccagcgcgcc
    10801 tcgggcggga tgattttctc ttctccagtg atcagcgcaa catgacgctc gcccttatgg
    10861 gccaccatgc ggtcgtaatt ctcgcgggcc agcaggcgga ggggaaagcc gatgatgccc
    10921 gaggcatgcc ccagcatgcg ctcgatggcg agatgggtct tgccggtgtt tgtcgggccg
    10981 agaacggctt tgacacgcgg agcaaaaccg gaatcgagac cgggcatggc aagatggttg
    11041 gatgttgggg cgcggaatgc aagcgg
//
LOCUS       DATGEM010000354         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_840742, whole genome shotgun sequence.
ACCESSION   DATGEM010000354 DATGEM010000000
VERSION     DATGEM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840742"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>4275
                     /locus_tag="VKQ27_11240"
     CDS             10..>4275
                     /locus_tag="VKQ27_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HLY89546.1"
                     /translation="MPVRAWLFAVAPTRHVLLVLSHHIASDGESMAPLLADIAFAYTA
                     RQRGNAPDWLPLPVQYADYTLWQRQCLGDSSDPGSLISTHVAYWKETLAGLPEQIDLP
                     SDRPRQSHASNRGDEVRFFIGTALHEGLVHIGKACGGTVFMALHASVAALLSKLGAGT
                     DVPLGTPIAGRTDHALEALVGFFVNTLVLRTDLSGDPSFRELLAHVRAVDLGAYSHQD
                     LPFEILVETLNPTRTTAHHPLVQVMVAMQNNDDVLPELPGVKESVHELQSPCAKFDLE
                     FHFEEKRTPDGQPKGIDALLTFATDLFERETALALTRRLMLLVQTLVADPDRRIGSIN
                     LLSDDERAQLAAWNATARPMADASLATLFERQAAKTPDAVALVCDGATLTYAELDTRA
                     NRLAHYLIRRGVRTQSHVALWLERSPELIVALLAVLKAGGAYVPLHNAHPVPRLRFVV
                     EQSAAMVVLTDRDLDAAEWPAMVIDVRGTAWHSEPCTAPGLEVDVQSLAYIMYTSGST
                     GTPKGIAVTQRNVAELALDHCWQDGAQQRVLLHSPHAFDASTYEIWVPLLSGQQILIA
                     PPEELDVAALAQLIAGRDVTAAFLTTALFRLLADEQPQCFARMRAVWTGGEAASPAAF
                     QAVLDRCPDLTLVHVYGPTETTTFATHYAMRAPHRVGASVPIGAPMDNTQVHVLDHAL
                     QPVPVGVCGELYIGGSGLARGYLQRPGLTAERFVANPFGHGTRLYRTGDLVRWLPDGQ
                     LDFMGRADHQVKIRGFRIELGEIEAALLRQSAVAQATVIAREDRPGVKQLVGYIVPSA
                     GSLIDPAALRRTLGEQLPDYMVPVALVVLEALPLTPNGKIDRKALPAPDFTPSSRRAP
                     RTPQEHVLAGLFAQVLGLTEVGVDDSFFDLGGDSISSIQLVSRARKAGLVFTPKDVFT
                     LQTVAALALKAGALHTPSAAPVESAIGAVPLTPIMRWWLEQDGPIGRFNQSMLLQVPA
                     LELAKLTTALEALLDHHDALRLRVQREPAGWSAQILAPGSVDVAACVQRVSIAGLNVA
                     QREALVAQHSDAAAARLDPEAGRMLQAIWFDAAHEPGRLLLVLHHLVVDGVSWRILVP
                     DLAAAYDGAALDPVGTSLRGWAQRLVTEAQSARRVAELPLWRDILQTEDPPLAARTLD
                     AAKDTVQTSAHLSLTLPAELTSELLTHVPALFHARINDVLLTAFALAIAHWRRQRLPD
                     TGLALRIDLEGHGREEIFDNLDLSRTAGWFTSLFPVRLDPGPIDLAQAMAGGSALGLA
                     LKRIKEQLRALPDHGIGFGLLRHLNPDTAQVLSAPRASQIAFNYLGRFAAGSAQHWST
                     APEASALQGGDAPARPLAHAIELNALTCDTAAGPELSAHWSWAANLFTHTDIQSLAKT
                     WFDLLAALVTHAQQPHAGGFTPSDLPLLTLQQAEIDQLQERLPA"
BASE COUNT          688 a         1469 c         1356 g          762 t
ORIGIN      
        1 acccgagaga tcccggtccg tgcctggttg ttcgccgttg cacccacgcg ccacgtactc
       61 ctcgtattga gtcatcacat cgcaagcgac ggcgaatcca tggccccgct gctggcagat
      121 atcgcgttcg cgtacaccgc acgccagcgg ggtaacgcgc ctgattggct gccgctgccc
      181 gtgcagtacg ccgactacac gctgtggcaa cgccaatgcc tgggtgactc gtccgatccg
      241 ggaagcctga tcagcacaca cgttgcctac tggaaagaga cgctcgccgg cctcccggag
      301 cagatcgatc tgccgagcga tcgtccgcgt cagtcgcacg ccagcaatcg cggtgacgaa
      361 gtccgttttt tcatcggcac agccttacac gaaggactcg tgcacatcgg caaagcgtgc
      421 ggcggcaccg tattcatggc gcttcatgcg tcggtagcgg cgctgttatc gaagctcggg
      481 gcaggcaccg atgtgccgct cggcacaccg attgccggtc gcaccgacca cgcgctcgaa
      541 gcgctggtcg gattctttgt gaacaccctg gtgctgcgca ccgacctctc tggcgatccc
      601 tcgttccgtg aactcctggc ccacgtgcgc gcagtcgatc tcggcgcgta ttcgcatcag
      661 gatctgccgt tcgaaatctt ggtcgagaca ctcaacccga cgcgcacgac ggcccaccat
      721 cccctggtgc aggtcatggt tgcgatgcag aacaacgacg acgtcctgcc ggaattgccc
      781 ggcgtgaagg agtcggtgca cgagctgcag tcgccctgcg caaagttcga cctcgagttc
      841 catttcgagg agaagcgtac tcccgacggc cagccgaaag ggatcgatgc actattgacg
      901 tttgccacgg acttgttcga gcgcgaaacc gcgctcgcgc tgacccggcg gttgatgctg
      961 ctggtccaaa cgttggtcgc ggatccggat cgacggatcg gttcgatcaa tttgctgagc
     1021 gatgacgaac gcgcgcagct tgccgcgtgg aatgcgacgg cacgaccgat ggccgatgcg
     1081 tcgctggcga cgttgttcga acggcaagcc gccaagacgc cggatgcggt ggcgctggta
     1141 tgcgatggtg cgacgctgac gtacgcggag ctcgatacgc gtgcgaatcg tttggcgcat
     1201 tatctgattc gtcgcggcgt acggacgcag tcgcacgtcg cgctctggct cgagcgatcg
     1261 cccgagctga tcgtcgccct gcttgccgtg ctcaaagccg gcggcgcgta cgtgccgctg
     1321 cacaatgcac acccggttcc gcgcctgcgc ttcgtggtcg aacaatcggc cgcgatggtg
     1381 gtattgaccg atcgcgatct ggacgccgcc gagtggccgg cgatggtgat cgacgttcgc
     1441 ggcacggcgt ggcactccga accctgcacc gcccccggac tcgaggtcga cgtgcagtcc
     1501 ctcgcctaca tcatgtacac ctcgggttcg accggcacac ccaaaggcat cgccgtcacg
     1561 cagcgcaacg tcgccgaact ggcactggat cactgctggc aggacggtgc gcagcagcgc
     1621 gtgctgctgc attcgccgca cgcgttcgat gcctcgacct acgagatctg ggtaccgttg
     1681 ctgtcgggcc agcaaatcct gatcgcgccg ccggaagaac tcgacgtggc ggcgctggcg
     1741 cagctgatcg caggccgcga tgtcacggcg gctttcctga caacggcgct gttccgcttg
     1801 ttggctgacg agcagccgca atgcttcgcc cgcatgcgcg ccgtatggac cggaggcgaa
     1861 gccgcctcgc ccgctgcgtt ccaggccgtg ctcgaccgct gccccgacct caccctggtg
     1921 cacgtctacg gtccgaccga gacgaccacg ttcgccacgc attacgccat gcgcgcgccg
     1981 catcgtgtcg gcgccagcgt gccgatcggc gcaccgatgg acaacaccca ggtccacgtg
     2041 ctcgatcatg cgctacaacc cgtgccggtg ggcgtttgcg gtgaactcta tattggcggc
     2101 agcggattgg cgcgcgggta tttgcagcgc cctgggctga ccgcagagcg tttcgtcgcc
     2161 aacccgtttg gccatggcac acgtctgtat cgcacgggcg atttggtgcg ctggctgccg
     2221 gatggccaac tcgacttcat gggccgtgcg gatcatcagg tcaagatccg cggcttccgc
     2281 atcgagctcg gcgagatcga ggcggcgctg ctgcggcaaa gcgccgtcgc gcaggcgacg
     2341 gtgattgcgc gcgaagaccg gcccggcgtc aagcagctgg tcggctacat cgtgccgtcg
     2401 gcgggcagcc tgatcgatcc cgccgccttg cgccgcacgc tgggtgagca gttgccggat
     2461 tacatggtgc cggttgcgtt ggtcgtgctc gaggcgctgc cactgacgcc caacggcaag
     2521 atcgaccgca aggcgctgcc ggcaccggac ttcacgccca gctcgcgccg tgcgccgcgc
     2581 acgccgcagg agcacgtgct cgccggcctg ttcgcgcagg tgctcggcct gactgaagtc
     2641 ggcgtcgacg attcgttctt tgacctcggc ggcgacagta tcagttcgat ccagctggtc
     2701 agccgcgcgc gcaaggcggg tttggtgttc acgccgaaag atgtgtttac gctgcagacc
     2761 gttgcggcct tggcgctaaa ggccggtgcg ctccacacgc cctcggctgc gcccgtggag
     2821 tcggccatcg gtgccgtacc gctgacgccg atcatgcgct ggtggctgga acaggatggc
     2881 ccgattgggc gtttcaacca gtcgatgctg ttgcaggtac cggcgctgga gttggccaag
     2941 ctcacgacgg cgcttgaggc actgctcgat catcacgacg ccttgcgcct gcgcgtgcag
     3001 cgtgagccgg cggggtggag tgcgcagatc ctggcgccag gcagcgtcga tgtcgcggcc
     3061 tgcgtgcagc gtgtttcaat cgctggattg aacgtggcgc aacgtgaagc actcgttgcg
     3121 cagcacagcg atgcggccgc ggctcgtctg gatcccgaag ccggacgcat gttgcaagcc
     3181 atctggttcg atgcggcaca tgaaccggga cgcttgttgt tggtgttgca ccatctcgtc
     3241 gtcgatggcg tgtcgtggcg aattctcgtg cccgatctgg ctgccgccta cgacggcgcg
     3301 gcgctcgatc cggtcggtac gtccttgcgc ggctgggcac agcgcctggt caccgaagcg
     3361 cagtcggcgc ggcgcgtggc agagctgccg ctgtggcgcg acatcctgca gaccgaggat
     3421 ccgccgctgg cggcgcgcac gctcgatgcg gccaaggata cggtgcaaac cagcgcgcat
     3481 ctgagcctca cgttgccggc agaactcacc tcggaattgc tgacccacgt ccctgcgctg
     3541 ttccacgcgc gcatcaacga tgtcctgctg acggcgttcg ccttggccat cgcgcactgg
     3601 cgccggcaac gcctgccgga caccggcctg gcgttgcgca tcgacctcga aggccacggt
     3661 cgcgaagaaa tcttcgacaa cctcgatctc tcgcgcaccg ccggctggtt caccagcctg
     3721 ttcccggtgc gcctggatcc cggcccgatc gatctggcac aagccatggc cggcggcagc
     3781 gctctgggcc tcgcgctcaa gcgcatcaag gaacaactgc gtgccctgcc cgatcatggc
     3841 atcggcttcg gcttgctgcg gcatctcaat cccgacaccg cgcaggttct cagcgcacct
     3901 cgcgcctcgc aaatcgcctt caactacctc ggccgcttcg ccgccggcag cgcgcagcac
     3961 tggtccacag ccccggaagc cagcgcgctg cagggcggcg acgcgcccgc gcgaccgctc
     4021 gcccacgcga tcgagctcaa cgccctcacc tgcgataccg ccgccggccc cgagctcagc
     4081 gcccactggt cctgggccgc caacctcttc acccacaccg acatccagtc cctcgctaaa
     4141 acctggttcg acctgctcgc cgctctcgtc acccacgcac agcagcccca tgccggcggc
     4201 tttaccccgt cggatctgcc gttgctcacg ttgcagcaag ccgaaatcga tcagctacag
     4261 gaacgcctcc ccgcg
//
LOCUS       DATGEM010000355         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_591614, whole genome shotgun sequence.
ACCESSION   DATGEM010000355 DATGEM010000000
VERSION     DATGEM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591614"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VKQ27_11245"
     CDS             <1..242
                     /locus_tag="VKQ27_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLY89547.1"
                     /translation="WADGIRVDTEGNVWCGFTGGEGEDGVAVFAPDGTMIGRILLPER
                     CANVCFGGAKRNRLFMAASQSIYAVYVEAQGVPGG"
     gene            complement(362..979)
                     /locus_tag="VKQ27_11250"
     CDS             complement(362..979)
                     /locus_tag="VKQ27_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLY89548.1"
                     /translation="MAPDPNKAAKSGPSPFIYGIIGLVVAVLIMGTGAFFWLSGGGSA
                     SLVGGPFTLEDGAGKQVSDRDFRGKYMLVYFGYTFCPDVCPTTLNEVADALDHLGTKA
                     NDLQPIFITVDPKRDTPAVVKQYTAAFSPKLIGLTGTPEQIAAVAQAYRVYYAEHRTG
                     PGPNDYSMDHSSILYLMGPDGKFIAPVRADESGKDMAADLSKLMS"
     gene            complement(1025..1765)
                     /locus_tag="VKQ27_11255"
     CDS             complement(1025..1765)
                     /locus_tag="VKQ27_11255"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89549.1"
                     /translation="MRHIALDHVGVVTRDLAALATQYDQLGFTLTPLARHADGRIGNR
                     CVMLRDSYMELLAVVDPAASSATLERFLARHPGVHILAFAVTDPHAEVARLQRAGVAA
                     PAVTGFERSSPDGSSEMARFELIQLPDQPEGRFNLVRHLTPDRLWQDRFLHHPNNAMS
                     LLSIELAVAEPLATAARLSRLLGCPAVPDPTHGFSLELTRGRLRLIEGAGPRITKLTI
                     STSDAGRAGIEIGASEAGGVALQFTKSD"
     gene            complement(1762..2424)
                     /locus_tag="VKQ27_11260"
     CDS             complement(1762..2424)
                     /locus_tag="VKQ27_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY89550.1"
                     /translation="MTDGTLYIGNRRYSSWSLRGWLAVKLAKLDVDVKMIHFSRPGPT
                     PAIGKLSPNGLVPYLEHRGARVWESLAVCEYCADVTPSLWPSDRIARAHARAMSAEMH
                     AGFLGLRQAMWMNLGRDFAGLGRTPEALADVARIEALWSDTRQQFAAGGRFLFGTEFT
                     AADAMFAPVVTRFITWKPDLTTATRAYCDAVRAHPLVGEWYEGAAAEPAEWLLPDYEK
                     AQ"
     gene            2516..>3118
                     /locus_tag="VKQ27_11265"
     CDS             2516..>3118
                     /locus_tag="VKQ27_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89551.1"
                     /translation="MTKVALSGASGNIGQVLRPALLRLGYPLRSASGRRPLTPLSAGE
                     DVLQGDLRDPAIVDRLLNGIDVLIHMAGTSVERPLPEVIENNLVGLNAVYEGARRHRV
                     KRIVFASSNHAVGMHSVDSKLNLDCDLRPDGFYGLSKVWGEAMSRMYWDKHGIEGICL
                     RIGTAIEKPTEFRHLSTWLGNDDLVQLIDRCIKVPDIGFLV"
BASE COUNT          576 a          978 c         1026 g          538 t
ORIGIN      
        1 gctgggcgga cggcattcgc gtcgacaccg aaggcaatgt gtggtgcggc ttcaccggtg
       61 gcgaaggtga ggatggcgtc gcggtcttcg cccctgacgg aacgatgatc ggacggatcc
      121 tgctgccgga gcgctgcgcc aatgtgtgct ttggcggggc aaagcggaac cggctcttca
      181 tggcggcgag ccagtcgatc tacgcggtct atgtcgaggc gcaaggcgtg ccgggcggtt
      241 agcccctgtc attgcgaccc cggcggaagc agggggaagc aatctcgaat gggaagggcc
      301 ggccaccgcc ccgctcctca caaagtccgc atgacagggc cgtttgcggc acgcccgctc
      361 gttacgacat cagcttgctc agatccgccg ccatatcctt gccgctttcg tcggcgcgga
      421 ccggggcgat gaacttcccg tccggcccca tcaggtaaag tatcgaacta tgatccatcg
      481 aatagtcgtt cggccccggg ccagtgcggt gttccgcgta atagacgcga taagcctgcg
      541 cgactgccgc aatctgctcc ggcgtcccag tcaggccgat cagctttggt gaaaacgcgg
      601 cagtgtactg cttcaccaca gcgggcgtgt cgcgcttcgg atcgaccgtg atgaagattg
      661 gctgcaggtc gttagccttg gtgccaaggt ggtcgagtgc gtcggcgacc tcgttgagcg
      721 tggtcgggca gacgtccggg cagaacgtgt agccgaaata gaccagcata tacttgccac
      781 ggaagtcgcg gtcgctgacc tgcttgccgg cgccatcctc cagcgtgaac gggcctccga
      841 ccagagaggc ggagccacca ccgctcaacc agaagaaagc gccggtgccc atgatcagca
      901 cggcaaccac aaggccgatt atgccgtaaa tgaaggggga agggccggat ttcgcggcct
      961 tgttcggatc gggagccatg gtggccggac cctagacctg cggaggcgac gcagatagcc
     1021 ccggctaatc ggacttagtg aattgtaatg cgacgccgcc ggcctcgctg gccccgatct
     1081 cgatgcccgc gcggcccgca tctgacgtcg atatcgtcag cttcgtgatc cgtggtccgg
     1141 cgccttcgat cagccgcagc cggccacgtg tcagttcgag cgagaagccg tgcgtcggat
     1201 cgggcacggc cggacatccg agcagacgtg acaggcgcgc cgccgtggcc agcggctcgg
     1261 cgaccgccag ctcgatgctg agcagcgaca tggcgttgtt cggatggtgc aggaagcgat
     1321 cctgccatag ccggtccggc gtcaggtgac gcaccaggtt gaagcggccc tcgggctggt
     1381 ctggcaactg gatcagttcg aagcgcgcca tctcgctgct gccatcaggg ctgctgcgct
     1441 cgaagccggt caccgcggga gcggcaacgc cggcacgttg cagccgtgcc acctcggcgt
     1501 gcgggtccgt caccgcaaac gccaggatgt ggacacctgg atgacgtgcg agaaaacgtt
     1561 ccagcgtcgc actgctcgcg gccggatcaa ccacggccag caactccatg tagctgtccc
     1621 gcagcatcac gcagcggttg ccgatacgcc catccgcgtg gcgcgccagc ggtgtaagag
     1681 tgaagccaag ctggtcatat tgcgtggcga gcgcggcaag atcgcgcgtc accacgccaa
     1741 catgatcgag tgcgatatgc ctcactgcgc cttctcataa tctggcagca gccattccgc
     1801 cggttcggcg gcggcaccct cgtaccactc gcccaccaag gggtgcgcgc gcaccgcatc
     1861 gcaataggcg cgcgtcgccg tggtcagatc cggcttccag gtgatgaacc gcgtcaccac
     1921 cggcgcgaac atcgcatccg cggcagtgaa ctcggtgccg aacagaaacc ggccaccggc
     1981 cgcaaattgc tggcgcgtat cggaccaaag tgcctcgata cgcgcgacgt cggcgagggc
     2041 ttccggcgtg cgcccaagcc cggcgaaatc gcgccccagg ttcatccaca tcgcctgacg
     2101 caagcccaga aatccagcat gcatttccgc gctcatggcg cgcgcatggg cgcgggcgat
     2161 ccggtcggat ggccacagcg atggcgtgac atcggcgcaa tattcgcaga cagcaagcga
     2221 ctcccagacg cgagcgcctc gatgttcgag atacggcacg aggccatttg gtgacagctt
     2281 gccgatggcc ggcgtcggtc cggggcggct gaaatggatc atcttcacgt caacgtcgag
     2341 cttcgccagc ttcaccgcga gccagccgcg cagtgaccac gacgaatagc ggcggttgcc
     2401 gatatagagt gtgccgtctg tcatcgttta ccctccatca acgcggtgtc gtcttgcatg
     2461 atcgaggggc gcggcccatg gatgggcgtg cccggtcgtc agcggaggaa catgcatgac
     2521 caaagtggca ttgtcaggcg ccagcgggaa catcggccag gttctgcgcc cggcgttgct
     2581 cagactgggc tatccgctgc gttcggcaag cgggcggcgg ccgttgaccc cactctcggc
     2641 cggcgaagat gtgcttcaag gtgatctgcg cgaccccgcg attgtcgatc ggctgctgaa
     2701 cgggatcgat gtgctgatcc acatggcagg caccagcgtc gagcggccgc tgcctgaagt
     2761 catcgaaaac aacctcgtcg gcctgaacgc ggtatacgaa ggcgctcgcc ggcatcgcgt
     2821 gaagcgtatc gtgttcgcca gttcgaacca tgccgtcggc atgcatagcg tggatagcaa
     2881 gctgaacctg gactgcgacc tgcggccgga tggtttctac gggctgagca aggtctgggg
     2941 tgaggcgatg tcccgcatgt actgggacaa gcacggcatc gagggcattt gcctgcgtat
     3001 cggcacggcg atcgagaagc cgacggaatt ccggcatctc agcacatggc ttggcaacga
     3061 cgacctggtg cagttgatcg atcgctgcat caaggtgccg gatatcgggt tcctggtc
//
LOCUS       DATGEM010000356        13028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_384629, whole genome shotgun sequence.
ACCESSION   DATGEM010000356 DATGEM010000000
VERSION     DATGEM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384629"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VKQ27_11270"
     CDS             complement(<1..665)
                     /locus_tag="VKQ27_11270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HLY89552.1"
                     /translation="MPAQTNQRIDGKRLWDLLMDMAQIGATPKGGVRRLTLTDVDKRG
                     RDRFRADCEAAGLTVRVDAIGNMFARRPGRDPNRLPVLFGSHLDSQPSGGKFDGALGV
                     LAGLEVMRSLNDLGIVTEAPLELVNWTDEEGSRFGHSLMGSGVWSGVFPLDKAYALTD
                     VEGVTVRTALDGISYRGDQPAAPFPADAYFELHIEQGPILEREVTDIGIVTGAQAMVW
                     YDAV"
     gene            733..1149
                     /locus_tag="VKQ27_11275"
     CDS             733..1149
                     /locus_tag="VKQ27_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89553.1"
                     /translation="MRATSVMLAVMLLAGCKLIDQTTFAPSPEAKAQAVEVPKADART
                     PLVSINFAQPDPDYQGLLRYALHAAAERDPSVEYDVVAMLPPNADVGAQQKHGLEVMR
                     AMIAQGVQANRIHLGLRSAAAGAEPEVRVYVRNLRG"
     gene            1245..2447
                     /gene="hemA"
                     /locus_tag="VKQ27_11280"
     CDS             1245..2447
                     /gene="hemA"
                     /locus_tag="VKQ27_11280"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HLY89554.1"
                     /translation="MHPFHAHCRDALDAIESQGRYRRFTPLRKQAERFPLYRRPDGSE
                     VLVWSSNDYLGMGGHPVVVTAACVAACDMGTGAGGTRNISGTSPAHDALEDELADLHG
                     KDAALLFTSGFVSNQAALSTILNSMPGWMVFSDEKNHASMIAGIKGSKATCRIFRHND
                     LGHLEELLREAPVAAPKLIAFESVYSMDADIAPIGAICDLATRYGAMTYLDEVHAVGM
                     YGPSGGGVSERDGVAARVDVIEGTLAKAFGCHGGYITGDAAVVDYIRSCAPGFIFTTS
                     LPPMVAAAALASVRHVREDAARRAKLFERAETLKRRLRAAGVPVLPSASHIVPVHVGD
                     AALCTAVSRRLLDEFGMYATPINYPTVPRGEERLRLTPTPLHTDAMMDRLIAALGVVL
                     GAERREAA"
     gene            3125..3763
                     /locus_tag="VKQ27_11285"
     CDS             3125..3763
                     /locus_tag="VKQ27_11285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLY89555.1"
                     /translation="MAFGDAVGQAINFGRQVATTGAYTLALPSLLHMKQKPAAWVFPR
                     YGKAIDEIVLHGTESKVSQVKSLDYLASKNDDKHSIHYWIGRDFGLLYQLVPEDKSTP
                     HAGNPDNVPKVEDHNFRSIGIEMYQWDPDVLRAKKLPVDFTDWQYDAVAQLVYDICRR
                     RGIKRSMVVGHGMINPVQRADPQGFDWPRFNRLLDQLSARLGGLLGDDFKLS"
     gene            3848..5008
                     /locus_tag="VKQ27_11290"
     CDS             3848..5008
                     /locus_tag="VKQ27_11290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HLY89556.1"
                     /translation="MRLNTGQGRRLRAGSPWVFSNELAMKPEYRQMPPGGLARIEGDD
                     GARFGTFMFNPHSLIAARLLDRDPAAVIDVEWLRGRLREAIALRERVCDTPYHRLVHA
                     EADRLPGLVIDRYDTVAVIQANAAGMDRLTPLIVEALTALLPLRAVVARNDSGARRQE
                     GLAEEVALPFGEDARAEVVEGGVRFPVDPLEGQKTGWFFDQRPNRDRVTALAEGARVL
                     DVFCHVGAFGVRCAVAGAREVLLVDSSAPALAQAEQAAALNGVAERVRTRRGDAFEVM
                     GELAGERFDLVVCDPPAFARSRKDAEAGLRAYGRMARTAASLVAPGGFLFVASCSHHA
                     PLEAWSTQIAFGLHRARREARIVYTGGAGPDHPVHPHLPETAYLKAQLIQVL"
     gene            5005..6501
                     /locus_tag="VKQ27_11295"
     CDS             5005..6501
                     /locus_tag="VKQ27_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89557.1"
                     /translation="MSQTQHSRKPFGGAVIAAISLSLLILAVWAALVATLSSLGDSDP
                     AGNGLEQAFAGFEIILVWILLAILLVVAGVQGAMTPPGVASAVVLLPASGVAAAVALG
                     LLADRATAPFVWPIVVPAVVPPVVVAFGFWALMPSLRAAVPARFAVGGAWGIVLLLCV
                     AMWPLSLVRDRAVQQAVDEQARWSAELAAMKADAPLWEWMRFLETRNRLDIDRALERI
                     RALERRQSDAEVMLDREDFPLRYLGSLDVRITQAICDKARGMLMRQVRALVPQTANAQ
                     PYSDVADKVAGALSAIQWLVGYGCPCEAEAQAWQTMAAAYRDTNFDVVSLGEYRDPKE
                     LGWAVRQDPAQFGMMLPQAPLMAWLNVAEVPALREQALAGAGRVAGRTDQAIVLLRQD
                     AAAMVLRALPVLDLDATPALCGAAMPAVYTTLTSVARPGSDDPRPYGVLLQQMGGDAP
                     LVALQWLASHGCDAGAAVEEAEGLVRAYQDSPQRAAMLANLARLPHRP"
     gene            6505..7644
                     /locus_tag="VKQ27_11300"
     CDS             6505..7644
                     /locus_tag="VKQ27_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89558.1"
                     /translation="MDPSRANNPLFGSNRFKLGVFSANCDGGLTMSLAPERWRADWDD
                     IVAMTRIADEAGLEFILPVAKWRGYQGKANIYGRSFETLTHGAALGALTKRIAIFSTV
                     HVPLVTPAFGAKAIATIDHVTHGRAGLNIVCGWNQEEFDLHGVTIDQHRRYEHGLEWF
                     RVWSRMLGGGPEFDWDGEFFHLKRLQTDPVSVQRPWPAVMSAGFSPKGRDFAAQAADV
                     LFTTMSELDQAPALLADIGAHVARYGRRVDVYTMSHVVCRPTRREAEEFYHYFAEEMA
                     DADGQAYYRRQRGPVVQDAAGNKIRRPLVNRLMRESGKRYDGAYPGAYPLVGTPDDIV
                     ADMVQMSELGLAGTSVAFLDYLNEIPFFVQEALPRLARLGLRREE"
     gene            7644..7982
                     /locus_tag="VKQ27_11305"
     CDS             7644..7982
                     /locus_tag="VKQ27_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89559.1"
                     /translation="MSAYLVVRAVVPEVARRDFDTWYRDEHLPDAVKAFAALRGWRGW
                     SRTDPSVHYAFYEFADVAAAEAVGSSPAIKKLIAEFDARWGTSVTRTREVIEVAVAGS
                     GPAMTGGNDG"
     gene            7975..9036
                     /locus_tag="VKQ27_11310"
     CDS             7975..9036
                     /locus_tag="VKQ27_11310"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLY89560.1"
                     /translation="MGNKIAFMGVGAVGGYAGAHMVQAGEDVTFIDPWPSHVEKMQRD
                     GLKISHIRDVPEFTVKVRAMHVTDAQQLAKEKPIDIAFVCMKSYDTEWATTLISQYLA
                     PGGFIVSLQNCMNEETIAGVVGWGKTVGCIASSITVDLCEPGHVRRAAGKSGTKHTVF
                     RTGEVHGRVTDRVKEIARLVGYADSALITENLWGERWSKLVTNVMGNGLSACTGMLGK
                     EMVQNDSIRRFSSRLGSEAIRVGQALGYELEEIMHLDPEIIAKAGEGDASATRVYDEH
                     RLGEVSKGGGEHRPSMGQDMVKGRRTEIEFLNGFVAKKGDEIGIATPANVVLTDIVKR
                     VERGDLKPDPKHITDLRLN"
     gene            9120..10163
                     /locus_tag="VKQ27_11315"
     CDS             9120..10163
                     /locus_tag="VKQ27_11315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY89561.1"
                     /translation="MRVFTRLPMHDWATTAQAARDAEAAGFDAVMTVELGHDVFAPLA
                     IAAMATGRVELTPSVAVAFPRSPTVLASQAWDLQANSRGRFVLGLGSQVKGHNERRFG
                     IAWTAPAPRLRDYVRALRAVWQCWETRGKLEFRSEHYTLTLMTPDFSPEPTGLPMVPV
                     TIAAVGEAMLRVAGQVADGVRLHPLCSRKYLEEVCLPQIEVGRQRGGGAREHFDVHGG
                     GFVVTGPDEATVAAGMEAARRRIGFYGSTRTYMPILALHGLEELGLKLHRMSVEGRWG
                     EMSALVSDDVVRIFAACGTYPEIAGAIGQRYGGAADSIDLEFPEAAPVGLRREVLDDI
                     RRIPHRFSGFAPR"
     gene            10176..11636
                     /gene="hpaB"
                     /locus_tag="VKQ27_11320"
     CDS             10176..11636
                     /gene="hpaB"
                     /locus_tag="VKQ27_11320"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HLY89562.1"
                     /translation="MPARTGRQYLDGLREQQREVWIGGERVKDVTTHPGLARGAQAIA
                     SLYDMQCDPKLQDQMTYTSPTSGDRVGLSFIQPRTREELEARRVMMLNWARTTCGMMG
                     RSPDFMNVTFAAWGAAADYWGRSKPEYGVNMRRYYEYIRENDLVLTHSLINLQRSRNI
                     SAQYKLEEGTALRAVKETSRGLVVKGARVLATLGPISDEIAVYSPRLGQYSEDHSPFA
                     LAFSIPCGTPGLRFLCRDSFDLGRSHFDHPLGSRFEEMDCIVFFDDVEVPWDRVFLYG
                     DVKLLNGAASGTHYSAHSSHQGAAKNLAKCEFVLGIALLMTEALGSTHLPHTEERIGE
                     LMLNTTMTRALMRAAEADAKIDEWGVMCPDPVTIESTRNLFMTAYPRMIEILQLLGSS
                     SFMITPSEVDFKGPLAGHIEQYLATDTIGARDRVSLFRLAWDVAGSSFGSRQVLYERF
                     FASDPLTRARALAAIYPKNEAMERVLTFLKQEDGRV"
     gene            11694..12212
                     /locus_tag="VKQ27_11325"
     CDS             11694..12212
                     /locus_tag="VKQ27_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89563.1"
                     /translation="MTYRPTHLGHVNLYVRNAERSRQWYEDVLGLHTYHFRPGRAAFM
                     SADKEKSHEVALMELGDDAPLQLKRQVGLNHAAFMMASLDDLKEAYYRLKEKGVKFDH
                     ISDHGLSLGIYFRDPDGNGLEVSYELPREKWPRQDSVFAPDVVNLGLFPGPWDEEIAA
                     QKAREAAKVPAE"
     gene            complement(12303..12965)
                     /locus_tag="VKQ27_11330"
     CDS             complement(12303..12965)
                     /locus_tag="VKQ27_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY89564.1"
                     /translation="MPSPIVIAGDPHGNFTPIIRACSAEDPGTLILLGDCELRVPLHH
                     MLGSLFALGWQIHWILGNKDTETATEFDHLAGDFPEGDIGGKVIEVAGMRIAGLGGVF
                     KPRIWHPPEAPSFNTRHEFMLSLPPREHWRGGLPLWHRDSIFPEDFARLAQHRFDVLV
                     THEAPSCHRHGFAAIDRLAEVTGARLIIHGHHHESYAARLNNGIDVRGLAVAETWVIE
                     IA"
BASE COUNT         2141 a         3543 c         4943 g         2401 t
ORIGIN      
        1 acggcatcat accagaccat cgcctgggcg cctgtcacga taccaatgtc agtgacttcg
       61 cgttccagga tcgggccttg ttcaatatgc agctcgaaat acgcatcggc cgggaatggg
      121 gcggcaggtt ggtcgccgcg atagctaatg ccatcgaggg ctgtgcgcac agtcacaccc
      181 tcgacgtctg tgagagcgta agctttatcc agcgggaaca cgcccgacca aacgccggag
      241 cccatcagcg aatggccgaa gcggctgcct tcctcgtcgg tccagttgac cagttcgagc
      301 ggcgcttcgg tgacgatgcc gagatcgttc aggctgcgca tcacttcgag tccggcgagc
      361 acgcccaacg cgccgtcgaa cttgccgccg ctgggctggc tgtcgagatg gctgccgaac
      421 aggaccggca agcggttggg gtcgcggccg gggcggcggg cgaacatgtt gccgatggcg
      481 tccacacgga ccgtgagccc ggcggcctcg cagtcggcgc ggaagcggtc gcggccgcgc
      541 ttgtcgacgt cggtgagggt gaggcggcgg acaccgccct tgggtgtggc gccgatctgc
      601 gccatgtcca tcaggaggtc ccagaggcgc ttgccgtcga tgcgctgatt ggtctgggcg
      661 ggcatggttg gcgtcctcgt gcggtgaggc ggcatcttgc ccgcgcagcg gttcgctgca
      721 agacagggag gtatgagagc gacctcggtg atgctggcgg tgatgctgct ggcgggctgc
      781 aagctgatcg accagaccac gttcgcgccc tcgccggagg ccaaggcgca ggccgtcgag
      841 gtcccgaagg cggatgcgcg cacgccattg gtctcgatca attttgccca accggacccc
      901 gactaccagg gcctgcttcg ctatgcactg catgcggcgg cggaacgcga tccgtccgtg
      961 gagtacgacg tggtggcgat gctaccgccg aacgccgacg tcggcgcgca gcagaagcat
     1021 gggctggagg tgatgcgggc gatgatcgca cagggcgtgc aggcgaatcg catccatctt
     1081 gggttgcgct cggcggcggc tggggcagag ccggaagtga gagtctacgt ccgcaacttg
     1141 cggggttaac gtttcttcat gctccgtaag ggattgtacg catgggtcgc ggtgtctttg
     1201 atggctaagg tgcgcaccgc gcgatactgg aggcttggct gaggatgcat ccgttccacg
     1261 ctcattgccg tgacgcgctc gacgcgatcg aatcgcaggg ccgctatcgc cgcttcacgc
     1321 cgctgcgcaa gcaggcggag cgtttcccgc tctaccgccg gccggacggc agcgaggtgc
     1381 tggtgtggtc gtccaacgac tatctcggca tgggcgggca tccggtggtg gtcaccgcgg
     1441 cgtgtgtcgc ggcgtgcgac atggggaccg gcgcgggtgg cacgcgcaac atcagcggca
     1501 ccagcccggc gcatgatgcg ctggaggatg agcttgctga cctgcatggc aaggatgcgg
     1561 cgctgctgtt cacgtcgggg ttcgtgtcga accaggcagc gctgtccacg atcctgaact
     1621 cgatgcctgg gtggatggtg ttctcggacg agaagaacca cgcctcgatg atcgccggga
     1681 tcaagggatc gaaggcgacg tgccgcatct tccggcacaa tgacctggga catctggaag
     1741 agctgctacg cgaggcgcct gtggcggcgc cgaagctgat cgcgttcgag tcggtgtatt
     1801 cgatggatgc cgatatcgcg ccgatcgggg cgatctgtga tctggcgacc cggtacgggg
     1861 cgatgacgta tctcgatgag gtgcatgcgg tgggcatgta cgggccgtcg ggcggcggcg
     1921 tgtcagagcg agatggcgtc gcggcccggg tggacgtgat cgaaggcacg ctggcgaagg
     1981 cgttcggctg ccatggcggc tacatcacag gcgacgcggc ggtggtggat tacatccggt
     2041 cctgcgcgcc ggggttcatc ttcaccacgt cactgcctcc gatggtggcc gcggcggcgc
     2101 tggcgagtgt gcggcatgtg cgcgaggacg cggcgcggcg ggcgaagctg ttcgagcggg
     2161 cggagacgct gaaacgtcgg cttcgcgcgg cgggagtgcc ggtgctgccg tcggcgagcc
     2221 acatcgttcc ggtgcatgtg ggcgatgcgg cgctgtgcac ggctgtgagc cggcggttgc
     2281 tggacgaatt cggcatgtat gcgacgccga tcaactatcc gacagtgcct cggggcgagg
     2341 aacggctgcg gctaaccccg accccgctgc acaccgacgc tatgatggac cggctgatcg
     2401 cggcgctcgg cgtggtgctg ggagcggagc ggcgcgaggc ggcgtagcgg caaaccggaa
     2461 ctgcctcctg aaagagggag gtaggcatcg gctgatacga ttggccgatg gccaccgtct
     2521 cgtccgcagc gcctggtcga aagcgaactg agcggtcgca cgttgctgct ccttcacttg
     2581 tccgggtggc tgtgcgctgg agtgactggc gcgcacatct ttatcatgtt ctatctgcca
     2641 ggatttcaat gtccgccgtc gaccttcaag agtagccttg gattttccgc cctcggcctt
     2701 acggtcgggg tctgcggggg gcgttcgcgt tatcggggct gctgatctgc ttgctgcggc
     2761 ggttcaacct gatcaattac actcatccgt cggtgcacgt tccgactggt gggagaaagc
     2821 attcgatttg ggcgatcggc ttttcgctgg cactattcgg gtgcattgcg attcccatca
     2881 ataattttgg gcattatttc tgcctgatgc ccgcgggcgt agtcattcgt tccggatatt
     2941 tggatgcgcc gcgacacctg gcatgggatg atctgttgcc ttgcggagca accgcgttca
     3001 atttgatttg tatacgtcat cgctacgata tgtgacgcca gtcacagctt ttaccgggca
     3061 aacggacgtt ccatcggcgg atgcggaggt ccggtcttgc gggcaccaaa cggtaggagc
     3121 gacgatggca ttcggtgatg cggtgggaca ggccatcaat ttcggccggc aggtcgcaac
     3181 cacaggtgca tatacgctgg cgttaccgag cctgctgcac atgaagcaga agccggcggc
     3241 ctgggtgttt ccgcgctatg gcaaggcgat cgacgagatc gtcctgcatg gcaccgaaag
     3301 caaggtaagc caggtcaaga gcctggatta tctcgcgagc aagaacgacg acaagcactc
     3361 gatccactac tggatcggcc gcgatttcgg cctgctgtat cagcttgtgc cggaggacaa
     3421 gtcgacgccg catgcgggca atcccgataa tgtcccaaag gttgaggacc acaatttccg
     3481 ctccatcggc atcgagatgt atcagtggga tcccgatgtc ctgcgggcga agaagcttcc
     3541 tgtggatttc accgactggc agtatgacgc cgttgcacag ctcgtttacg acatctgccg
     3601 acggcgcggg atcaagcgca gcatggtggt cggacacggc atgatcaatc cggtgcagcg
     3661 agccgaccca caggggttcg actggcctcg gttcaaccgc ctgctggatc agctttcggc
     3721 gcgattgggc ggtctgctgg gtgatgattt caagctgagc tgatctcggc catttgacct
     3781 gtcggctatc gccgatacaa cggctcgctg aacagcgagc ggatcgcgtc atttggatcg
     3841 tcccatattg cggttgaata cagggcaggg acggcggctg cgcgctggct ccccctgggt
     3901 gttctccaac gaactcgcga tgaagcccga gtatcggcag atgccgccgg gcgggttggc
     3961 gcgaatcgag ggcgacgatg gtgcccgctt cgggacgttc atgttcaatc cgcacagcct
     4021 gatcgcggcg cggctgctgg atcgcgatcc ggcggcggtg atcgatgtgg aatggctgcg
     4081 cgggcggtta cgtgaggcga tcgcgctgcg ggagcgcgta tgcgacacgc cgtaccatcg
     4141 gctggtgcat gcggaagccg accggctgcc ggggctggtg atcgatcggt acgacaccgt
     4201 ggcagtgatc caggcgaacg ctgcggggat ggatcggctg acgccgctga tcgtggaggc
     4261 gctgactgcg ctgctgccgc tgcgggcggt ggtggcacgg aatgattccg gcgcacggcg
     4321 gcaggaggga cttgccgagg aggtcgcgct gccgttcggc gaggatgcgc gggccgaggt
     4381 ggtggagggc ggcgtacggt tcccggtcga tccgctggag gggcagaaga ccggatggtt
     4441 cttcgatcag cggccaaatc gtgatcgggt gacggctctg gctgagggcg cacgggtact
     4501 ggacgtgttc tgccacgtgg gcgcgttcgg ggtacgctgc gcggtggcgg gagcgcggga
     4561 ggtgctgctg gtggacagca gcgcgccagc ccttgctcag gccgagcagg cggctgcgct
     4621 gaacggcgtg gccgagcggg tgcggacacg gcgaggcgat gcgttcgagg tgatgggcga
     4681 actggcgggc gagcggttcg atctggtggt gtgtgatccg ccggcgttcg ctcggtcccg
     4741 gaaggacgcc gaggcggggc tgcgggcgta tgggcggatg gcgcgcacgg cggcgtctct
     4801 ggtggcgccg ggcgggtttc tgttcgtcgc gtcgtgcagc catcacgctc cgctggaggc
     4861 gtggagcaca cagattgcgt tcgggctgca ccgggcacgg cgggaggcgc ggattgtgta
     4921 tacgggcgga gcggggcctg accatccggt tcatccgcat ctgccggaga cggcttatct
     4981 gaaagcgcag ctgatccagg tgttgtgagc cagacccagc attcacgtaa gccgttcggc
     5041 ggcgcagtca ttgctgcgat cagtctgagc ctgctgatcc tggcggtgtg ggcggcgttg
     5101 gtcgcgacgc tcagcagcct gggcgacagc gatccggcgg ggaatgggct ggagcaggcg
     5161 ttcgccgggt tcgagatcat cctggtgtgg atactcctgg ctatactgct cgtcgtggcg
     5221 ggcgtgcagg gggcaatgac gccgccaggc gttgcgtcgg ctgtggtgct gttgccggca
     5281 tccggtgtcg ctgctgctgt tgcactgggg ctgctggcgg atcgggcgac agcaccattc
     5341 gtgtggccaa tcgtggttcc ggcggtcgtg cctccggttg tggtggcgtt tgggttctgg
     5401 gcgttgatgc cctcgttgcg cgcggcggtg ccggcgcgtt ttgcggtggg tggggcgtgg
     5461 ggcatcgtgc tgctgctgtg cgttgcgatg tggcctctgt ccctggtgcg cgatcgggca
     5521 gtgcagcagg cggtggacga gcaggcgagg tggtcagctg agctggcggc gatgaaggca
     5581 gacgcgccgc tgtgggagtg gatgcggttc ctggagacac gcaaccggtt ggatatcgac
     5641 cgggcgctgg agcgtatccg tgcgctggag cggcggcaga gcgatgcgga ggttatgctg
     5701 gatcgcgagg atttcccgct gcggtatctg gggtcgctcg atgtgcggat aacgcaggcg
     5761 atttgcgaca aggcacgggg aatgctgatg cggcaggtgc gggcgctcgt gccgcagacc
     5821 gcgaacgctc aaccgtactc ggatgttgct gataaggtgg cgggcgcgtt gagtgcgatc
     5881 cagtggctgg ttggatacgg ttgtccctgc gaggcggagg cgcaggcgtg gcagacgatg
     5941 gcggcggcgt atcgcgacac gaatttcgac gtggtgagtt tgggtgagta tcgcgatccc
     6001 aaggaattgg ggtgggcggt gcgacaggac ccggcgcagt ttggcatgat gctgcctcag
     6061 gcgccgctga tggcgtggct gaatgtggcc gaggtgccgg cgttgcggga gcaggcgttg
     6121 gctggtgcgg gtcgggtagc cgggcggacc gatcaggcga tcgtgttgct gcgtcaggat
     6181 gcggcggcga tggtgctgcg ggcgctgcct gtgcttgatc tggatgcgac gccggcgttg
     6241 tgcggcgccg caatgccggc cgtatatacg acgctgactt cggtggcacg gcctggatcg
     6301 gatgaccctc ggccatatgg tgtgttgttg cagcagatgg gaggcgatgc gcctctggtg
     6361 gcgctgcagt ggctggcctc gcatggctgc gatgcgggtg ccgcggtcga ggaggcggag
     6421 gggttggtgc gggcgtatca ggactcgccg cagcgggcgg caatgcttgc caatctcgcg
     6481 cggttgccgc acaggccgta ggccatggat ccgtcgcgcg ccaacaatcc tctgttcggc
     6541 tcgaaccgct tcaagctggg cgtgttcagt gccaattgcg atggcgggct gacgatgtcg
     6601 ctggcgccgg agcggtggcg cgccgattgg gacgatatcg tcgcgatgac gcgcatcgcc
     6661 gacgaggccg ggcttgagtt tatcctgccg gtggcgaaat ggcgtggtta tcaggggaag
     6721 gcgaacatct acgggcgttc gttcgagacg ctgacgcatg gcgcggcgtt gggagcgctg
     6781 acaaagcgga tcgcgatctt ctccaccgtg catgtgccac tggtcacacc ggcgttcggc
     6841 gcgaaagcga tcgcgacgat cgaccatgtg acgcatggac gggcggggct gaacatcgtg
     6901 tgcggatgga accaggagga gttcgatctg catggtgtga cgatcgatca gcatcggcgg
     6961 tatgagcacg ggctggagtg gtttcgtgtg tggtcgcgga tgctgggggg cgggccggag
     7021 ttcgactggg atggcgagtt tttccacctg aagcgattgc agacggatcc ggtgtcggtg
     7081 cagcggccgt ggccggcggt aatgtcggcg gggttctcgc cgaaaggacg cgatttcgcc
     7141 gcgcaggcgg ccgacgtgct gttcaccacg atgagcgaac tggaccaggc gccggcgctg
     7201 ctggcggata tcggcgcgca cgtcgcccgg tatgggcgtc gggtcgacgt gtatacgatg
     7261 tcgcatgtgg tgtgccggcc gacgcggcgc gaggcggaag agttctatca ctattttgcc
     7321 gaagagatgg cggatgcgga cgggcaggca tactaccggc ggcagcgtgg gcctgtggtg
     7381 caggatgcgg cggggaacaa aatccggcgg cctctggtga accggttgat gcgtgagagc
     7441 ggcaagaggt acgacggggc gtatcctggc gcgtatccac tggtgggcac gccggacgac
     7501 atcgttgccg atatggtgca gatgagcgaa ctggggctgg cggggacttc ggttgcgttc
     7561 ctcgactacc tgaacgaaat tcccttcttc gtgcaggaag cgctgccgcg gctcgcgcgg
     7621 cttggcctgc gccgggagga gtgatgtcgg cttatctggt ggtgcgcgcg gtggtgcccg
     7681 aagtggctcg gcgcgacttc gatacgtggt atcgcgacga gcatctgccg gacgcggtga
     7741 aggcatttgc cgcgctgcgc ggctggcgag gctggagccg gaccgaccct tccgtgcact
     7801 acgcgttcta tgagtttgcc gatgtcgcgg ctgcggaggc ggttgggtcg tcgcctgcga
     7861 tcaagaagct gattgcggag ttcgatgcgc ggtggggcac gagtgtgacg cgcacgcgcg
     7921 aggttatcga agtagcggtg gccgggtcgg gcccggccat gacaggagga aacgatgggt
     7981 aacaagatcg cattcatggg tgtcggcgcg gtgggcggct atgccggcgc gcatatggtg
     8041 caggcgggcg aggacgtgac gttcatcgat ccgtggccgt cgcatgtgga gaagatgcag
     8101 cgcgatgggc tgaagatcag ccatatccgc gatgtgccgg agttcaccgt gaaggtgcgc
     8161 gcgatgcatg tgacggacgc gcagcagctt gccaaggaaa agccgatcga catcgcgttc
     8221 gtctgcatga agtcgtacga cacggaatgg gcgacgacac tgatcagcca gtacctggcg
     8281 ccgggcgggt tcatcgtttc gttacagaac tgcatgaacg aggagacgat tgccggcgtg
     8341 gtgggctggg gcaagacggt ggggtgtatc gcgagctcga tcaccgttga tctgtgtgag
     8401 ccggggcacg tgcggcgcgc ggcgggcaag agcggcacga agcacacggt gttccgcacg
     8461 ggcgaggtgc atgggcgcgt caccgatcgg gtgaaggaga tcgcccggct ggttggatat
     8521 gcggacagcg cgctgataac cgagaacctt tggggcgagc ggtggtcgaa gctggtgacc
     8581 aacgtgatgg gcaacgggct gtcggcgtgc accggaatgc tcggcaagga gatggtgcag
     8641 aacgactcga tccggcggtt ctcctcgcgg ctgggaagcg aggcgatccg cgttggccag
     8701 gcgctggggt atgagttgga ggagatcatg cacctcgatc cggagatcat cgcgaaggcg
     8761 ggcgagggcg atgcgtcggc gacgcgggtt tacgacgagc accggctggg cgaggtgagc
     8821 aaagggggcg gcgagcaccg gccctcgatg ggccaggaca tggtgaaggg acggcggacg
     8881 gagatcgagt tcctgaacgg gttcgtggcg aagaaggggg acgagatcgg gatcgcgacg
     8941 ccggcgaacg tggtgctgac ggatatcgtg aagcgggtgg agaggggcga cctgaagcct
     9001 gatccgaagc acatcacgga tctgcggctg aactagcccc gtgtcattgc gagcggagcg
     9061 aagcaatctc gtgcgggatc gcttcgtcgc tatgcttctc gcaatgacaa ggtgacacaa
     9121 tgcgcgtgtt tacccgcctc ccgatgcatg actgggccac aacagcacaa gcggcacgcg
     9181 acgcggaggc ggctggattc gacgcggtaa tgacggtcga gttggggcac gacgtgtttg
     9241 ccccgctcgc gatcgccgcg atggctacgg ggcgggtgga gctgacgccg tcggtggcgg
     9301 tggcgtttcc gcgcagcccg acggtgctgg cgtcgcaggc ttgggacctg caggcgaact
     9361 cgcgcgggcg gttcgtgctg gggctgggca gtcaggtgaa ggggcacaat gaacggcggt
     9421 tcggcattgc gtggacagcg ccggcgcctc ggctgcggga ctatgtgcgg gcgttgcgcg
     9481 cggtgtggca gtgctgggag acgcgcggaa agctggagtt ccgcagcgag cattacacgc
     9541 tgacgttgat gacgccggat ttctctcccg agccgacggg gttgccgatg gtgccggtga
     9601 cgatcgcagc ggtgggcgag gcgatgctgc gcgtggccgg gcaggtggcg gatggggtgc
     9661 ggctgcaccc gctgtgctcg cggaagtatc tcgaggaggt gtgcctgccg cagatcgagg
     9721 ttggccggca gcgtggcggc ggggcgcgcg agcatttcga tgtgcatggc ggcgggttcg
     9781 tggtgacagg gccggatgag gcgacggttg ccgcggggat ggaggctgca cggcggcgga
     9841 tcgggttcta tgggtctaca cgtacgtata tgcccatcct ggcgctgcat ggtttggagg
     9901 agctggggct gaagctgcac cggatgtcgg tggaagggcg ctggggcgag atgagcgcgc
     9961 tggtgtcgga tgacgtggta cggatctttg ccgcatgcgg gacgtatccg gagatcgccg
    10021 gggcgatcgg gcagcggtat ggcggggctg cggattcgat cgatctggag tttcccgagg
    10081 cggcgccggt ggggctgcgg cgggaagtgc ttgacgacat caggcgcatc ccgcatcgct
    10141 ttagcggatt tgcccccaga taaaggagac gcgccatgcc ggcacgcacg ggacgccagt
    10201 acctcgatgg gctgcgtgag cagcaacgcg aggtctggat cggcggtgag cgcgtcaagg
    10261 atgtgacgac gcatccgggg ctggcgagag gcgcgcaggc gatcgcgtcg ctgtacgaca
    10321 tgcagtgcga tccgaagctg caggatcaga tgacctacac gtcgccaacg tcgggcgacc
    10381 gggtgggact gtcgttcatt cagccgcgca cgcgcgagga gctggaagcg cggcgcgtga
    10441 tgatgctgaa ctgggcgcgg acaacgtgcg gcatgatggg gcggtcgccc gacttcatga
    10501 acgtgacctt cgccgcatgg ggagcggcgg cggattactg ggggcgcagc aagccggaat
    10561 acggcgtcaa tatgcgtcgc tactacgagt atatacgcga gaacgacctg gtgctgacgc
    10621 actcgctgat caacctgcag cgcagccgga atatctcggc gcagtacaag ctggaggaag
    10681 gcacggcgct gcgggcggtg aaggagacga gccgcgggct agtggtgaag ggcgcgcggg
    10741 tgctggcgac gctggggccg atctcggatg agatcgcggt gtattcgccg cggctgggac
    10801 agtactccga ggatcacagt ccgttcgcgc tggcgttctc catcccgtgc ggcacgccgg
    10861 ggctgcggtt cctgtgccgc gacagcttcg acctggggcg gtcgcatttc gaccatccgc
    10921 tcgggtcgcg cttcgaggag atggactgca tcgtgttctt cgacgatgtg gaagtgccgt
    10981 gggaccgcgt gttcctgtat ggcgacgtga aactgctgaa cggggcggcg agcggcacgc
    11041 attactcggc gcattcatca catcaggggg cggcgaagaa cctggccaag tgcgagttcg
    11101 tgctggggat cgcgctgctg atgaccgagg cactgggcag cacgcatctg cctcatacgg
    11161 aagagcgcat cggcgagctg atgctgaaca cgacgatgac gcgtgcgctg atgcgagcgg
    11221 cggaggcgga tgcgaagatc gacgaatggg gcgtgatgtg tcccgatccg gtgacgatcg
    11281 agagcacgcg caatctgttc atgaccgcgt atccgcggat gatcgagatc ctgcagttgc
    11341 tgggttcgtc gagcttcatg atcacgccga gcgaggtgga tttcaaaggg ccgctggcgg
    11401 ggcatatcga gcagtatctg gcgaccgaca ccatcggcgc ccgcgatcgg gtcagcctgt
    11461 tccgcctggc gtgggatgtg gcggggagtt cgtttggcag ccggcaagtg ctgtacgagc
    11521 ggttctttgc ctcggacccg ttgacacggg cccgcgcgct ggcggcaatc tatcccaaga
    11581 acgaagcgat ggaacgcgtg ctgacgttct tgaaacagga ggatgggcgg gtctagcccg
    11641 cccatgacga tcgaaggccg ggctacgtcc ggaatcaaat ggaggactca cccatgacgt
    11701 atcgaccgac gcatctggga cacgtcaacc tgtatgtgcg caatgcggag cgctcgcggc
    11761 aatggtacga ggatgtgctg gggctgcaca cgtatcactt ccggccgggt cgcgcggcgt
    11821 tcatgtcggc ggacaaggag aagtcccacg aggtcgcgct gatggaactt ggcgacgatg
    11881 cgcccctgca gttgaagcgg caggtggggc tgaaccatgc ggcattcatg atggcgtcgc
    11941 tggatgatct gaaggaggcc tactaccggc tgaaagagaa aggggtgaag ttcgaccaca
    12001 tctccgatca cgggttgtcg ttggggatct atttccgcga tccggacggg aatgggctcg
    12061 aggtgtcata tgagctgccg cgcgagaagt ggccgcggca ggacagcgtg ttcgcgccgg
    12121 atgtggtcaa tctcgggctg ttccccgggc cgtgggatga ggagatcgcg gcgcagaagg
    12181 cgcgtgaggc ggcgaaggtg ccggcggaat aagcgcaaca cggcctgatt ccaagggtgt
    12241 ttcgatcgcg tttcaccacg actgtgcatg gccggtcatg acgtatccgt ggcgtcgtca
    12301 tgtcaggcga tttcgatgac ccaggtctcc gcgaccgcca ggccgcgcac gtcgatgccg
    12361 ttgttcagcc ttgcggcata agattcgtgg tggtgaccgt ggatgatgag gcgcgcgccg
    12421 gtgacttcgg cgaggcggtc gatcgcggcg aagccgtggc ggtggcagga cggcgcttcg
    12481 tgcgtgacca gcacgtcgaa gcggtgctgg gcgaggcggg cgaagtcttc cgggaagatc
    12541 gagtcgcggt gccacagcgg caggccgccg cgccagtgtt cgcgtggcgg caggctgagc
    12601 atgaattcat ggcgggtgtt gaagctgggc gcttcgggtg ggtgccagat gcgcggcttg
    12661 aacacaccac ccaggccggc gatgcgcatg ccggcaacct cgatgacctt gccgccgata
    12721 tcgccctcgg ggaaatcccc cgcgagatga tcgaactcgg tcgcggtttc agtgtccttg
    12781 ttgccgagga tccagtgtat ctgccagccg agcgcgaaca gtgacccgag catgtggtgc
    12841 aacggcacgc gcagttcgca atcgccgagc aggatcagcg tgccgggatc ctcggcgctg
    12901 caggcacgga tgatcggcgt gaagttcccg tgcggatcac ccgcgatgac gataggcgat
    12961 ggcacgcttc acacctcatg aacgggtgcc gccgtagccg agcagccggg cgacggggcc
    13021 gatcgtcc
//
LOCUS       DATGEM010000357         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1389531, whole genome shotgun sequence.
ACCESSION   DATGEM010000357 DATGEM010000000
VERSION     DATGEM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389531"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VKQ27_11335"
     CDS             complement(<1..535)
                     /locus_tag="VKQ27_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLY89565.1"
                     /translation="MAVGTVATRGRAARRRWSQTALGSWVTTADHKRIGIMYIYTGIF
                     FFFIGGIEAALIRLQLARPDGTVLSPEAYDQVFTMHGTTMIFLFVMPTMAGFGNYLVP
                     LMIGARDMAFPRLNAFSFWISAFGGLLLYFSYLGGDGLYGMGSAPDVGWFAYAPLTAK
                     VFSPGHSTDYWTLSLLLT"
     gene            complement(602..1603)
                     /gene="coxB"
                     /locus_tag="VKQ27_11340"
     CDS             complement(602..1603)
                     /gene="coxB"
                     /locus_tag="VKQ27_11340"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLY89566.1"
                     /translation="MVLSACEKSPSTLDPAGPIATSEAGIFWFILGLSTFIFVVVTAV
                     LLYSVFRFRARPDSPAPRQIHGNTKIEIAWTVAPSVILFIVLGITIGTMFALAQPADA
                     NTVRVRAVAHQWWWEFQYTAPNGQTVDVADELHMPVGTVVEISLFSDNVIHSMWVPEL
                     GGKTDVIPGHDNKMWLKADKAGRYRGECAEYCGTQHAHMDFVAVAEDPGTFQTWLTNE
                     QATVQKPTDGTPDANALAVFQSAGCSGCHVINGISPANIRLIGPNLTHFGSRRLIAGW
                     VLENNPTNLRKWILQAQQVKDGSDMPSFDGSVPTVNGGKALTSDQLDSLVNYLESLQ"
     gene            complement(1760..2383)
                     /locus_tag="VKQ27_11345"
     CDS             complement(1760..2383)
                     /locus_tag="VKQ27_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLY89567.1"
                     /translation="MKLRLVSRIIVLALVVVVGGGILLHNRTQAANSPASRGLQGTLL
                     NDPAPTFTLRDQTGTSVSLAALRGRPVVLTFLYTHCPDACPLTAEKLHTAAQALGADA
                     TKVSWLAVSIDPAGDTPASATAFVAAHHLTGRLQFLLGGASQVSLIWNAYHIPVQPEP
                     ASPGKPTVVDHLLGLYLIDGAGRERVYMGDDFDPAVLSANLRLLMAG"
BASE COUNT          489 a          853 c          816 g          429 t
ORIGIN      
        1 cagtcaacaa cagtgacagg gtccaatagt ccgtgctatg acccggcgag aagaccttcg
       61 cggtcagcgg agcataggcg aaccacccaa catctggcgc ggaacccatg ccataaagcc
      121 catcgccgcc cagataactg aagtacagca gcaaaccgcc aaacgccgat atccagaaac
      181 taaatgcatt cagccgcgga aacgccatgt cccgcgcccc gatcatcagc ggcacaagat
      241 agttgccgaa gcccgccatc gtcggcatga cgaacaggaa gatcatggtc gtcccatgca
      301 tggtgaagac ctgatcgtac gcctccgggc tgagcaccgt gccgtcgggc cgcgccagtt
      361 gcaagcgaat gagcgccgcc tcgatgccgc cgatgaagaa gaagaagatg cccgtgtaga
      421 tgtacatgat gccgatccgc ttgtgatcgg ccgtcgtcac ccacgagccg agcgcggtct
      481 ggctccagcg tctgcgcgcc gcgcgcccgc gtgtcgcgac tgttccgact gccatggttc
      541 ctctcctgct gctgtgttca gttcctcagc cctatggctg gatgtcgttc aagcgtctgt
      601 gctactggag gctctccaga tagttgacga ggctgtcgag ctggtcggag gtcagcgcct
      661 tgccgccgtt gacggtggga accgagccgt cgaagctcgg catgtcactg ccgtccttca
      721 cctgctgcgc ctgcagaatc cacttgcgca ggttcgtcgg gttgttctcg agcacccagc
      781 ccgcgatcag cctgcggctg ccgaaatggg tcaggttcgg gccgatcagg cggatgttgg
      841 ccgggctgat gccgttgatc acgtggcagc cggagcagcc ggcgctctgg aagacagcga
      901 gcgcgttggc gtcgggcgtg ccatcggtcg gcttctgcac cgtcgcctgt tcgttggtca
      961 gccaggtctg gaatgtgccg ggatcctcgg cgacggcgac gaaatccata tgcgcgtgct
     1021 gcgtgccgca gtactcagcg cattcgccgc gatagcgacc cgccttgtcc gccttcagcc
     1081 acatcttatt gtcgtggccg gggatgacgt cggtcttgcc gcccagttcc ggcacccaca
     1141 tgctatggat aacgttgtcg gagaacagcg agatctcgac gaccgtgcca accggcatgt
     1201 gcagctcgtc ggcgacatcc accgtctggc cgttcggcgc ggtgtactgg aattcccacc
     1261 accattgatg cgcgacggcg cgcacgcgca cggtgttggc gtcggccggc tgcgccagcg
     1321 cgaacatcgt gccgatggtg atgccaagca cgataaagag gatgaccgat ggcgccaccg
     1381 tccacgcgat ctcgatcttg gtgttgccgt gaatctgccg cggcgccggt gaatccggcc
     1441 gcgcgcggaa gcggaacacc gaatagagca gcaccgcggt gaccacgacg aagataaacg
     1501 tcgagagtcc gaggatgaac cagaagatgc cggcctcgct ggtggcgatg gggccggccg
     1561 ggtccagggt gctgggggat ttttcgcagg cgctcagcac caccgacgcc agcagcgcca
     1621 gcagcgagac actcgcccac cgtctcatct tcgcacggaa ccgcatggtg agattcctcg
     1681 acctcctcag tatgccggcg cttccaccgc gatgtctacg tcagcggatg cgccgggccg
     1741 gccaatagca agagaacgct cagcccgcca tcagcaagcg cagattcgcg ctcagcacgg
     1801 cgggatcgaa gtcgtcgccc atatagacac gctcgcggcc cgccccgtca atcaggtaca
     1861 ggccgagcag atgatccacg acggtcggct tgcctggcga tgctggctcc ggctgcaccg
     1921 gaatgtgata ggcgttccag atcaggctga cctgggatgc gccgcccagc aagaactgga
     1981 gcctgccggt caagtggtgc gccgcgacga acgccgtcgc cgaggctggc gtgtcgcccg
     2041 ccggatcaat gctcaccgcc agccaggaaa ccttcgtggc atccgcgccg agcgcctgcg
     2101 ccgccgtgtg cagcttctcc gctgtgagcg ggcaggcatc cgggcaatgc gtatacagga
     2161 acgtcagcac taccggccgc ccgcgcagcg ccgccagcga cacgctcgtg ccggtctggt
     2221 cgcgcagcgt gaaggtcggc gccggatcgt tcagcagcgt gccttgcagc ccccgcgacg
     2281 ctggcgaatt ggcggcttgc gtgcgattgt gcagcagaat gccgccgcca acgacgacca
     2341 ccagggccag cacgatgatg cgtgagacga gccgcagctt catgggcgag ccttaaccct
     2401 tggcgcgggg atcaccgcag tacccgatcc actaccatcg ccgcgaagag cagcgccaaa
     2461 tagtagttcg agaaccagaa cagcgtgcgc gcccgcttca gcgtcttctc gcgggccagc
     2521 aggaacgcta gcgccagcaa gcccaacccc agcagcagcg ccgcggccag gtacaggtat
     2581 cccatcg
//
LOCUS       DATGEM010000358         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1334123, whole genome shotgun sequence.
ACCESSION   DATGEM010000358 DATGEM010000000
VERSION     DATGEM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334123"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1558)
                     /locus_tag="VKQ27_11350"
     CDS             complement(260..1558)
                     /locus_tag="VKQ27_11350"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLY89568.1"
                     /translation="MVTRAPFLKARYSGYHHRQVPKEDTELTHVGPETPCGEYMRRFW
                     QPICYSDELKDLPLAVKVLGEELVVFRDGSGAVGLLERHCSHRGTSLEFGLVGAKGIR
                     CCYHGWLFDVDGAILETPGEPANSTLKDRLCHGAYPVHEAHGLVFAYMGPPEQMPDFP
                     EYDSLSRAGYRIIPGQKYFYPCNWLQILENTMDAVHTAFLHTIVSGSVFTDEFGVLPE
                     LDFTETPVGIIYIATRRVGENVWARMVENVLPNLQQVAPVWEDGQQAHPFSGPMMSRW
                     IVPLDDTNTMLIELRHVSATDGVVTPAWWADRYTALPGQVAADTYEESQRRPQDFEAQ
                     VSQRPIAVHGLEHLGTTDRGISMFRNQIRHGIRAVKAGNDPVGLFRNGSGAIPTYCND
                     TVVRVPPAKTAELDKKLLRETGRRLAEGYIEAPPLLAAAE"
     gene            1681..>2684
                     /locus_tag="VKQ27_11355"
     CDS             1681..>2684
                     /locus_tag="VKQ27_11355"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLY89569.1"
                     /translation="MSNGPFRAAGYPWRLHWGQQVIEQSLRESTDRARAKRAFVICSP
                     SVNRRTDIIRRIEAALGGVYAGVYDGIAKDSTYASVLAATTAARVANADALIAVGGGS
                     VIVATRAVAIFLAESGDPFQLMTQYPEGKSAYSPRLLAPKPPIINVPTTPTSAMNRAG
                     TGLKNPDLDHRMEYFDPKTRPQAIFLDHEALLTAPPDLIRSTATTVFATNVAAMSQTD
                     INPLAEGDRNHAFHLANRAYPRLMGELDNPALRIDLALAAFLQNRTEDDGSRRFRGGA
                     FAGSYAVSTALHVRYPHVGQGEATSVVHAPKIRITEKPHPASTRQVTEALNLWRDTMT
                     P"
BASE COUNT          507 a          960 c          747 g          470 t
ORIGIN      
        1 cagagggccg gggagttcga gccgacatgc gccgccgcac cgcgcataat gaggctccgc
       61 tccgcggccc tctgcggtta tccgctgtcc tccgcacaaa taaagatggc tccgccaccc
      121 gcacgaacac caccggccga agccgacgac gacaatggct agccgagctt tcgggcgcgc
      181 caggctactc ccggcgccgc cactgctgac cgtgcatcgg cgagcaatcg ttcagcagta
      241 cgagcaagcg cccaactgct tattccgcag ccgccagcaa cggtggcgct tcgatatagc
      301 cctcggcgag ccgccggccg gtctcacgca gaagcttctt gtcgagctcg gccgttttcg
      361 ccggtggcac gcgcacgacc gtgtcgttgc aatacgtcgg gatcgcgccg ctgccgttgc
      421 gaaacaggcc caccggatcg ttgcccgcct taaccgcccg aatgccgtgc cgaatctggt
      481 tgcggaacat cgagatcccg cgatccgtcg tgccgagatg ttcaaggccg tgcacagcga
      541 tcggccgctg cgacacctgc gcctcaaaat cctgcggccg gcgttggctt tcctcgtagg
      601 tatcggcggc aacttgccca gggagcgccg tgtaccgatc agcccaccag gctggtgtca
      661 caacgccgtc agtcgcactg acatggcgca gttcgatcag catggtattg gtatcgtcca
      721 gcggcacgat ccaacggctc atcatcggcc cactgaacgg atgcgcctgc tgaccatcct
      781 cccagaccgg agcgacctgc tgcaggttgg gcaatacatt ctcgaccatc cgcgcccaga
      841 cattctcgcc gacgcgacgc gtcgcaatgt agatgatgcc gacgggcgtc tcggtaaaat
      901 ccaattccgg caagacgccg aactcgtcgg tgaacaccga gccgctgacg atcgtgtgga
      961 gaaatgcggt gtgtaccgca tccattgtgt tttccaggat ctgcagccag ttgcacgggt
     1021 agaagtattt ctgtccagga ataatccggt agcctgcccg gctcaagctg tcatactccg
     1081 ggaaatccgg catctgctcg ggtggcccca tataggcgaa caccaaccca tgtgcctcgt
     1141 gcaccggata ggcgccatga cagagccgat ccttcagcgt gctgttcgcg ggctcgccag
     1201 gtgtctcgag gatcgcgcca tccacgtcga acagccagcc atggtagcag cagcgtatcc
     1261 ctttggcgcc aaccagaccg aactccagcg acgtgccgcg gtgcgagcaa tgccgctcca
     1321 acaggccaac cgcgccgctg ccgtcgcgga atacaaccag ctcttcgccg agcaccttga
     1381 ccgccagcgg taggtctttc aactcgtcgg agtagcatat cggttgccag aagcgtcgca
     1441 tgtattcgcc acacggcgtc tccggcccaa catgcgtcaa ctcggtgtct tccttgggaa
     1501 cctgacgatg atggtaaccg ctgtaacgcg ccttcaagaa cggagccctc gtcaccattg
     1561 caagcctcct ggattcctgg gtttatcgtc gggcggtggt tctcgcttcc ctcgttcggt
     1621 gccggcataa tgggacagcc gaggcacagt gtgcaatctt gttgaaaagg acggcgccca
     1681 atgagtaatg gccccttcag ggcggcaggc tatccatggc gtttgcactg gggccaacag
     1741 gtgatcgaac agagcctgcg ggaatccacg gatcgggcgc gcgccaagcg cgccttcgtc
     1801 atttgctcgc cctcggtgaa ccggcgcaca gacatcattc gccgcatcga ggctgccctc
     1861 ggcggcgtat atgcaggcgt ctatgacggc atcgccaagg actccacata tgcctcggtc
     1921 ctcgccgcaa ccaccgctgc gcgggtcgcc aatgccgatg cgctgatcgc cgtaggtggc
     1981 ggcagcgtca tcgttgccac ccgtgccgtc gccatcttcc ttgcggagtc aggcgacccc
     2041 ttccagctca tgacccagta tccggaggga aaatcggcct acagccctcg tctgctcgcg
     2101 cccaaaccgc cgatcatcaa cgtccccacc acgcccacca gcgccatgaa ccgcgcgggc
     2161 accggcctga agaaccccga cctcgaccac cgtatggaat atttcgaccc gaagacccga
     2221 ccccaggcga tcttcctcga tcacgaggca ctgctcaccg caccgcccga cctcatccgc
     2281 tccaccgcca ccacggtgtt cgccactaat gttgccgcca tgtcgcagac cgacatcaac
     2341 ccgctggccg agggcgaccg caaccacgcc ttccacctcg ccaaccgggc ctatccgcgc
     2401 cttatgggtg agctcgataa cccagcgctg cgcattgacc tcgcactcgc cgccttcctg
     2461 cagaaccgca ccgaggacga cggctcccgc cgcttccgag gcggcgcctt cgctggcagc
     2521 tacgccgtct ccacggctct tcacgtccgc tacccacatg tcggccaggg cgaggccacc
     2581 tcggtcgtcc atgcccccaa aatccgcatc acggagaagc cccaccctgc ctccacccgc
     2641 caggtaaccg aagccctgaa cctctggcgc gacaccatga cccc
//
LOCUS       DATGEM010000359        23578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1494168, whole genome shotgun sequence.
ACCESSION   DATGEM010000359 DATGEM010000000
VERSION     DATGEM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 23578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23578
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494168"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VKQ27_11360"
     CDS             <1..866
                     /locus_tag="VKQ27_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HLY89570.1"
                     /translation="VRERKTWRRGLRLCFWSGHSHGRYSGSTWYADQNWDELDRRCAV
                     HVNVDSTGGVGAMVLKNAAAAPELIALAGDAISEQAGSEYAGKRMSRSSDQSFWGIGI
                     PAMFGALSEQTPAPVKMRNALGWWWHTPHDLLDKIDEKNLVRDTKVYVHTLCRLLTDT
                     VLPLDFARHADALLEELAALRTALADRLSIDSLITAAETLRTNAQGDHSDAALMRASR
                     ALDPVYYTSGDRFAHDPALPLPAWPVLDPLRKLAKVAPGTDSGRFLAVSATRGRNRLA
                     YALREAIAALS"
     gene            complement(1001..1462)
                     /locus_tag="VKQ27_11365"
     CDS             complement(1001..1462)
                     /locus_tag="VKQ27_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLY89571.1"
                     /translation="MIPPAERYFEDYTVGLVDEFGEVEVTAEEIVGFARRYDPQAMHT
                     DAEASARGPFGGLIASGWHTAAMVMRLFVEHYLSRVATLPAPGIDELRWVLPVRPGDR
                     LRVRVTVTEARRSRSKPDRGIVRSLVEVLNQRDEVVMTQRPMNLMRCRTDG"
     gene            complement(1459..2907)
                     /gene="boxB"
                     /locus_tag="VKQ27_11370"
     CDS             complement(1459..2907)
                     /gene="boxB"
                     /locus_tag="VKQ27_11370"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HLY89572.1"
                     /translation="MGEGTTIDYDGLIPNNVGLADDARVRRALETWHPGYIDWWRQMG
                     PEGFQDRLVYLRTAIGVGSEGWAKFGYVKMPEYRWGVLLAPAVEDRKIGFGAHKGEPV
                     WQEVPGEYRAMLRRLLVIQGDTEPASVEQQRHLGVTAPSQYDMRNLFQVNVEEGRHLW
                     AMVYLLQKYFGRDGREEAEELLRRRSGDQDSPRMLGAFNEATPDWLSFFMFTFFTDRD
                     GKMQLAALAQSGFDPLSRTCRFMLTEEAHHMFVGETGVQRIVQRTCDAMREAGIDDPA
                     DVERVRALGVIDLPTLQRKANLHFSLTLDLFGNEISTNAANAFNAGLKGRYHEDRLTD
                     DHVLGNDTYPVLRLKDGEIAREDVPALSALNMRLRDDFVDDANAGVTRWNRVLERSGL
                     EFRLRLPHVAFNRRIGEFAAIHADTEGHLLNGDRWDAQRDTMLPTNDDNSYIESLMRP
                     VNEPGAFASWIAPPTRGIDNKPGDFEYVKIEA"
     gene            complement(3005..4621)
                     /gene="boxC"
                     /locus_tag="VKQ27_11375"
     CDS             complement(3005..4621)
                     /gene="boxC"
                     /locus_tag="VKQ27_11375"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HLY89573.1"
                     /translation="MIDFQIDPSRYRHWRLAFDGPIATLTMDVDPDGGLFEGCELKMN
                     SYDLGVDIELADAVQRLRFEHPEVRTVILRSGKEGVFSAGANIRMLAKATHGHKVNFC
                     KFTNETRLAIEDACDNSGQRWLAAVSGSAAGGGYELAMAAEHIMLVDDRRSGVALPEL
                     PLLAVLPGTGGLTRLTDKRMVRRDRADVFCSTEEGVRGRRAVDWRLVDEVVPPSSWEA
                     KLRERALALAEKSDRPVDAKGVVLAPLQRTLSDDGNVYTHVRATFDRGQRRAELTVRG
                     PGEVSGLDWALLLARELDDAILHLRLNEPTLGLIVLRSEGDPAAVLAADEELEARKDD
                     WLVREIRLYWKRVLKRLDMTSRSLIALIEPGSCFAGTLAELLFAADRSVMFAGTRSSD
                     NRGPATIALSALNFGAYPMGNGLTRVATRFLGEPDSFARARECVGERLEAEDAEAAGL
                     VTLAYDDTDWSDEVRLMLEERASFSPDALTAMEANLRFAGPETMESRIFGRLTAWQNW
                     VFQRPSAVGAEGSLKRYGSGLRPNYDTERV"
     gene            complement(4618..5391)
                     /locus_tag="VKQ27_11380"
     CDS             complement(4618..5391)
                     /locus_tag="VKQ27_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89574.1"
                     /translation="MIIDGRSLETKWWGPGPEVAPTLVLLHEGLGCVTLWRDFPEALV
                     AATDCGVFAYSRFGYGGSDPVTLPRPVTYMHDEALSILPHVLDAAGVRRGILLGHSDG
                     GSIAAIYAGAVHDARVLGVVMLAAHFFVEDLNVAAIGAIRRSYEEGDLRARLARYHRD
                     VEVAFRGWNDSWLDPRFRDFDITDQVASIRVPILALQGAEDPYGSDEQLRVLERAARC
                     DVETRVIDGAQHSPHLEARQATLDAIVPFVRNVLLKATA"
     gene            complement(5388..6947)
                     /locus_tag="VKQ27_11385"
     CDS             complement(5388..6947)
                     /locus_tag="VKQ27_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HLY89575.1"
                     /translation="MLSGAGNAVEYFVDRHVSEGRGGSLMCRDPWRSLSYGELEVSTR
                     RFAGALRGAGIAREQRVVLLLLDTIDFPVAFWGAMRAGVVPVPINTLLTHEMVGYILA
                     DSRATGLVISAPLVEPLLPVLHSVPELRRIIVAAPDGSAAAVDDARAVGFGAFIEGGD
                     PATATVDAPADEVAFWLYSSGSTGAPKGVRHVHGSLRATADSYGAQVLQIRADDVMFS
                     AAKAFHAYGLGNSLTFPMSIGASAVLLPERPTPDAVLETMARHQPTIFAGVPTLFAAM
                     LANPHIGPKAGSQRLRRCISAGEALPADIGRRWSDMVGVDILDGIGSTEMLHIFLSNR
                     ADDVRYGTSGKPVPGYEALILDEHGGRVPNGEEGELVVRGPSAADGYWNQRGKTRRTF
                     RGEWTHTGDTYIRDHEGYFRFRGRTDEMLKVSGVWVSPFEVEEALVSHPAVLESAVIG
                     AQDHDGLTKPKAFVVLQDAARQQDAETVKELLKTHVKERIGVWKYPRWIEFVDGLPKT
                     ATGKIQRFRLQ"
     gene            complement(7034..7573)
                     /locus_tag="VKQ27_11390"
     CDS             complement(7034..7573)
                     /locus_tag="VKQ27_11390"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLY89576.1"
                     /translation="MVRPGAIEVWSVNLVTAAVVALLVYAALADIVARIIPDGIVVWV
                     AVLGLATRVMAGLPSVAASIVAALLSLALLVVLHSRGVMGGGDVKLAAAIVLGLPIGS
                     IYWFVMITALAGGVLALLHLVLRAAIRTAPHAPPRGAPLLRRAVTAEWWRIARRGSLP
                     YGVAISCGGIWAILASRGG"
     gene            complement(7801..8901)
                     /locus_tag="VKQ27_11395"
     CDS             complement(7801..8901)
                     /locus_tag="VKQ27_11395"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89577.1"
                     /translation="MSELLDRPVRPTPVNVRALLDRSAHVTRVRARAPLRISFAGGGT
                     DLAFYADTFGGCVLNATIGMFAYCSIKLRDDQKVRFVALDHEQEWEFDGDAVGAPPGL
                     LAMHAAVHRRIVGQYLGGACLPVTVMTSSDALPGSGLGSSSTLVVAIIEAYRELLALP
                     LGEYDVANLAYQIERVDVGQAGGRQDQYAATFGGINFMEFAGEQTVVNPLRIRSATLT
                     ELEAMLVLYSTGISRESAKIITEQSASLRSGHAASLEAMHQMRRDTYVMKDALLHNDL
                     QAFASVLGRSWEAKKRTASAISNGEIDRVHALAMESGAWSGKVSGAGGGGFLFFLVPP
                     ERRPGLLRVLEREPGQTRICGFTKQGAQAWRV"
     gene            complement(8894..9397)
                     /locus_tag="VKQ27_11400"
     CDS             complement(8894..9397)
                     /locus_tag="VKQ27_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HLY89578.1"
                     /translation="MQFDPLPLPGAFVVAPERHADARGYFARTFCSDEFAAHGLAGSF
                     VQCSTSFNVRRGTLRGMHYQRPPYEEVKLVRCTRGVVLDVLLHRESGRWHAVELSEQN
                     GLAVYIPAGFAHGFQTLLANSEVFYQMAAAHHPAAAAVIRWDDPAYAIAWPIRPPILS
                     ERDAGHE"
     gene            complement(9397..10614)
                     /locus_tag="VKQ27_11405"
     CDS             complement(9397..10614)
                     /locus_tag="VKQ27_11405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY89579.1"
                     /translation="MTCRFCGAPLRRKFVDLGRAPLSNACLTAEQLRLPEPHYPLCAY
                     VCEECLLAQVEEVVSAEQIFGEYLYFSSYSDAWLRHADAYAKRMTDELRLDAQSLVVE
                     VASNDGYLLQYFRQRGVGVLGVEPAANVAAVAIGKGIATEIAFFDIETACRLAERGKA
                     DLICANNVLAHVPDLNGFVAGFPVLLKTSGIVTAEFPHLQRLIAETQFDTIYHEHFSY
                     FSLLVVDRLFARHGLYVFDVDRLPTHGGSLRVHACHVGANRQRTARFHAVLAEERAAG
                     LDRIETYECFGEAVIDAKCALLEFLIKARRGGELVVGYGAPAKGNTLLNYCGVGPELL
                     AFTVDRSPHKQGRFLPGSQIPVFSPERILQIRPDYVLILPWNLRDEIVGQMQAIRDWG
                     GRFVVPIPRAQVL"
     gene            complement(10611..11687)
                     /gene="rfbG"
                     /locus_tag="VKQ27_11410"
     CDS             complement(10611..11687)
                     /gene="rfbG"
                     /locus_tag="VKQ27_11410"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HLY89580.1"
                     /translation="MRAVQPLLNPRQDFWQGRRVLVVGHTGFKGSWLSVWLHRMGASV
                     VGLSLPPPTEPSLFALAGLDAQVETHIGDIRDRATVAAALARWRPEVVFHLAAQALVR
                     RSYREPVETFATNVMGTVHVLEAIRATPSVRCTVVVTSDKCYEDREWPWPYREIDAVG
                     GREPYSASKGCAELVASSYRRSFYPAIATARAGNVIGGGDWAEDRLLPDCIRALTAGA
                     SIAIRNPHAVRPWQHVLEPLCGYLLLAERLAENPARFGDAWNFGPPDEDARPVGWIAS
                     NLIERWGDHASWEIVSGDGPHETETLKVDASKARMRLGWTPRLPLEEALRWTVDWYRR
                     QAKGEPALALTGAQIVKYCGCAPA"
     gene            complement(12045..13262)
                     /locus_tag="VKQ27_11415"
     CDS             complement(12045..13262)
                     /locus_tag="VKQ27_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89581.1"
                     /translation="MRLSAGQRQAWLLLAACFVTFSVGAACMHSYTVFLIAFIEAFGW
                     TRAESSIAYSVSQMITGATSPLVGVLVDRLGPKRLVLIGGALLAVGLLASSQVHSLWQ
                     VILLYGVLMTLGANCLGLVVFVPILSRRFVRHRGTAVAVVQSANGFARAFSAPLSQVL
                     IAGLGWRGAYLAQGGFMAVAVLPLAAMFRSSDADNPTASSQAGLPSGWTLRLAARTPH
                     FWLLFLVYVFTGLGSFLVALHQLAFAVTIGFDKLYAAEVLGLGAFLSLPGVIVTGWMS
                     DYIGRELSAVVTYGTSIFGVVCAMLISSPDQHLLLWLHSCFFGLTWGARGPAITAKTA
                     DLFPGPRLGTILGAITIGTGVGSAAGSWLAGWSFDMTGNYHVAFALSIFFYVCGGVAF
                     WLLRRPPALAVAD"
     gene            complement(13326..14558)
                     /locus_tag="VKQ27_11420"
     CDS             complement(13326..14558)
                     /locus_tag="VKQ27_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89582.1"
                     /translation="MSDIAVVPAQGEFRQGWPVVLACFATAVFAWGFGFYGQSVYFAD
                     LHATRGWPSSLIASATTVYYLAGGLMLAYVHRAMALLGPRLLLAVGAVVLGVGAIWLG
                     RVTAPWQLYAAGLVMAVGWSATTTTAIATTLAHWFDRRRGLALSLALNGASGGGFIVT
                     PILARSVRMFGLGPGVTWLVLCGLAILLPLILVGVGRVASIEQSASPRPSVRRETGHL
                     PAFTTQGEALRSVRVWSIALPFALALAAQVGFIVHQLAFLRPQLGNEGAANAVAATAL
                     AAAVGRLAMAPLIDRLNQRVASAASFASQAAALVLMLLLPQQPVALYAGSIVFGVSVG
                     NVITLPALIVQREFAAQSFGLVIGLISMVGYTLLAFGPTLLGLAHDATGGYSAALLLC
                     IALQFAGAVIVLIRPRRG"
     gene            14639..15559
                     /gene="gcvA"
                     /locus_tag="VKQ27_11425"
     CDS             14639..15559
                     /gene="gcvA"
                     /locus_tag="VKQ27_11425"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HLY89583.1"
                     /translation="MSLPPLNALRAFEATARHLSMKEAATELSVTPGAVSQLVRGLEQ
                     RLGTQLFRRSNRALVLTESGQSYFVPIRHAFRQITEATRRLQATPNAGVLTVSAPPAF
                     AESWLVPRLGTFRTHHPDIELNIATTRRLANFVADGVDVAIRHGLGTYPGLRCDRIAT
                     IAMITVCSPRFLAALPRRPRTPADLLHISLLHEAERQEWALWFQAQGVTDVGHTASSG
                     ISFDDQMLLIRAAASDQGVALVTETLARRELDNGNLVRVLDVAWPQEFSYWLVCPRAT
                     AEVPKIVAFRDWLISQRARQARRATPNRTP"
     gene            complement(15507..16409)
                     /locus_tag="VKQ27_11430"
     CDS             complement(15507..16409)
                     /locus_tag="VKQ27_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HLY89584.1"
                     /translation="MQMPLPEAIYLPFESGPYRMSMSLTTVPESAWFEIDERYVDEMR
                     ERRELLAVRHGDVFGALPGSEAARGEALREVVANLCEHAPQWFARDGDVVRNALTGES
                     WELAAAPCDPLEVAGRLVQEDLCIIQNGADGPRFTAAVLCFPSRWRLNEKLGKPLTSV
                     HGPVPHYAERLAAPVDRFMARVKVGHIASRLNWSVLDDPALYQPTGKWREATNARITP
                     ENAGDSLYLRVERQTLRRLPQSGAILFGIRVHSYQLARAITRPEHAARLAEAVRALPE
                     ATMHYKSLRAFGSALLAWLDARAG"
     gene            16523..17434
                     /locus_tag="VKQ27_11435"
     CDS             16523..17434
                     /locus_tag="VKQ27_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLY89585.1"
                     /translation="MPDVRIMAEKFGFPEGPVAMPDGAVILTEIAGRCCTRIGPDGSR
                     STIGNAGGGPNGLALGPDGALYCCNNGGGKYPSGHWMAIGPADDYDGGCIQRIDPKTG
                     DTRVLYTHVNGHKLSAPNDLVFDTNGGFYFTDLGKRFARTRDHGGLYYALPDGSKVVE
                     VAYPVLSPNGCGLSPDGKVLYVADTEPARLWAFDITTPGVVKKQGWPSPHGGRLIAGL
                     GGFQRFDSLAVQANGDVAVATLHAGCITVISPDGKIVRQVTMPDEFPTNICFGGADMR
                     TAYVTLSATGRLGSLQWPEPGLKLNFN"
     gene            17496..17726
                     /locus_tag="VKQ27_11440"
     CDS             17496..17726
                     /locus_tag="VKQ27_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLY89586.1"
                     /translation="MSDRFEGTARELGGRVQQKVGEFAGDAKTQASGLYNQAAGQAEQ
                     QVAKLSDVIKDQPITSALVAIGIGYLLGRFTA"
     gene            17791..20019
                     /gene="glgX"
                     /locus_tag="VKQ27_11445"
     CDS             17791..20019
                     /gene="glgX"
                     /locus_tag="VKQ27_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HLY89587.1"
                     /translation="MPSNARKSRLSEGSPNPRGATWDGLGVNFALFSANATKVELCLF
                     DDAGRTELERIELPEYTNEVWHGYLPDARPGTIYGYRVHGQHDPRNGHRFNPNKLVLD
                     PYAKAFVGNLRWDNALFGYTIGSKDEDLSLDRRDNARFMPKCRVIDPAFTWGRERRPD
                     VPWDRTIIYETHVRGHTKLHPAVPERLRGTFSGLGRKEVVEYIRSLGVTSVELLPIHA
                     FVNDRHLLERGLTNYWGYNSIGFFAPDPRYSAVPAFVFSEFKEMVARLHDAGLEVILD
                     VVYNHTAEGNERGPTLSFRGIDNASYYRLLPDNLRYYINDTGTGNTLNLSHPSVLQMV
                     TDSLRYWVTEMHVDGFRFDLGTILAREDHGFVETHGFLQSCQQDPVLSQVKLIAEPWD
                     IGPGGYQVGGFSPGWAEWNDKFRDTVRAFWKGDDGKLPELATRLAASADAFNRRGRKP
                     WSSVNFITAHDGFTLHDLVSYNDKHNEANGENNQDGHSDNLSWNHGVEGETDDPDIKA
                     LRCRQMRNLLATLLLSQGTPMLLAGDEFGRTQKGNNNAYAQDNEISWIDWNIGESGGA
                     LIEFVRRAIAVRNAFPILRRSRFLTGEYNAEFDVKDVRWLTPVATDMEEEQWRDGNAR
                     CFGMLMDGRAQATGIKQPSLDATVLVVLNAHHDVVNFTLPEVLGGQAWRCLLDTNVPD
                     LTAAPRITSGNEYQVTGRSLVLFAMQPDNAQSVALTRAREALRQVAENPLADLLVEET
                     AN"
     gene            complement(20149..21726)
                     /locus_tag="VKQ27_11450"
     CDS             complement(20149..21726)
                     /locus_tag="VKQ27_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89588.1"
                     /translation="MKRRTFVRTGLAASAAAALPRFSIAQPANARVLKFVPQANLTVL
                     DPIITTAAVTINHGWMVFDTLFGVNAQLQPKPQMAEGYTVSDDGRTCLIKLRDGLKFH
                     DGEPVLARDCAASLARWAARDTFGQTAAKSVESWGTADDKTVKITLKRPFPLLITGMA
                     MQPSFIMPERLAKTDPFKGVTEMIGSGPFRFIKSDFVAGSGAAWEKFDGYVPRQEKPE
                     WTSGGKVVEFPRIEWKVIPDASTASAALQTGEVDWYEQAAEDLVPLLRKNGEIVLGPS
                     NPQGYVGGMRFNTIQPPFNDVRLRRAVLAAVNQADYMRAIMGEDTSAWRICRSQYPCG
                     TMYGTEVNLPVQQGDLEAAKKMIKEADYNGAKAVIINPTDFPTIGPFGDITYDMFKKL
                     GINAELADTDWGTVVQRRASKEPVEKGGWSVFHTWFTGGFILNPVLTAPFRGQGTAGW
                     FGWYDDTKVEELTQEWLDATDDDSRKKIAAAIQAENYAQAATITLGQFQIPTAYRKSL
                     VGKLECNGPLFWNVKRA"
     gene            22113..>23578
                     /locus_tag="VKQ27_11455"
     CDS             22113..>23578
                     /locus_tag="VKQ27_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HLY89589.1"
                     /translation="MAGIPAIRVSTAQPSATAKMARSTYQDLFDASLSQYAAPAFSAS
                     FVTGSAPFCSLMLPITMGFDAYRSVPTSAATTTSPTAAKATSTALYLQEAGDTSLISV
                     NDIHQGQIGDCFLLASIGEIALWHPSAIMNMIHANADGTETVTLHLAASGQLPTYGTT
                     QFRATTVTVTNVFPSYAVNNGATQDVVNGVKEIWVQVLEKAVATLGGGYNYIANGGNP
                     MIAMEELTGQDTRYISSPASLTFSMLQGHMAAGDLIVMDTYSTGLLPYNLVNNHAYMF
                     ESVTVVNGTPMIQLGNPWGYNQPQLIPLSQLSRGIAEIDIGNFTDSNLITGGPGNDTV
                     TLSALVTNASVDLGAGNDTLTLANGTNSATVANTETVIGGTGSDAITLATAANNASVD
                     LGAGNDTLAFGSFTNSASVARVETLTGGSGNDTITLGSALTAAMSVDLGAGSNKLTLA
                     NGGNTGTVKNVATLVGGTGSDTLTLGGALVNGSVDLGSG"
BASE COUNT         4689 a         8470 c         6544 g         3875 t
ORIGIN      
        1 cagtgcgaga acgcaagacg tggcgccgcg gcttgcggct gtgcttctgg tccggccatt
       61 cgcacgggcg ctactccgga tccacctggt acgccgacca gaattgggac gaactggatc
      121 gacgctgcgc cgtgcacgtg aacgtcgact ccactggcgg cgttggagcc atggtgctga
      181 agaacgccgc ggccgcgccg gagcttatcg ctcttgccgg cgatgcgatc agcgaacagg
      241 ccggttccga gtatgccggc aagcgcatgt cgcggtcgtc ggatcaatcg ttctggggga
      301 tcggcattcc cgcgatgttt ggcgcgctca gtgagcagac gcccgcaccc gtgaagatgc
      361 ggaatgcact cggctggtgg tggcatacgc cgcacgatct gctcgacaag atcgatgaga
      421 aaaaccttgt gcgggatacg aaggtctatg tgcatacgct gtgccggttg ctgaccgaca
      481 ccgtgctgcc actggatttc gccaggcacg ccgacgcgct gctggaggaa ctcgccgcgt
      541 tgcgcaccgc attggctgat cggctctcga tcgacagcct gatcactgcg gccgagacac
      601 tacgaaccaa cgctcagggc gaccattcgg acgccgcact gatgcgggcg tcgcgcgccc
      661 tcgacccggt gtactatacg tccggcgacc gtttcgcgca cgatccggcg ctcccattgc
      721 cggcgtggcc ggtgctggat ccgcttcgca agcttgcgaa ggtcgccccc ggcaccgaca
      781 gcggccgctt cctggcagtc agtgcgaccc gcggccgcaa tcgcctggcc tatgcactga
      841 gagaagcgat cgccgcattg agttgacgtg tcacgacgct ttgagccgac gtcccgtcga
      901 cacgttgagt tgacctgtca ttgcgaggac cccggcgaag gccgggggac gaagcagtct
      961 cgtccagttt gctccgtcgc cgctcttcac gctgagcgct tcatccatca gtcctgcacc
     1021 gcatcaggtt catcggccgc tgcgtcatca cgacctcgtc cctctggttc agcacctcca
     1081 ccaggctgcg cacaatgccg cggtccggct tgctccgcga ccgccgcgct tccgtcaccg
     1141 tcacccggac gcgcaacctg tcgccggggc gcaccggcag tacccaccga agctcatcga
     1201 tgccaggcgc aggcaatgtc gccaccctcg acaggtaatg ctctacgaac agccgcatga
     1261 ccatcgccgc ggtgtgccaa ccgctggcta tcaggccgcc gaatggacca cgcgccgacg
     1321 cctccgcatc ggtgtgcatc gcctgcggat cgtaacgccg cgcgaagccc acaatctcct
     1381 cggcagttac ctccacctca ccgaactcat ccaccaggcc aaccgtatag tcctcgaagt
     1441 accgctcggc cggtggaatc atgcctcgat cttcacgtac tcaaaatcgc ctggcttgtt
     1501 gtcgatgcca cgtgtcggcg gcgcgatcca cgaagcaaac gcaccaggct catttaccgg
     1561 ccgcatcagg ctttcgatgt agctgttatc gtcattcgtc ggcagcatag tatcgcgctg
     1621 tgcatcccat cgatcgccat tcagcagatg gccttccgtg tccgcatgga tcgccgcgaa
     1681 ttcgccaatc cggcgattga acgcgacgtg tggcagacgc agacggaact cgaggccact
     1741 acgctccagc acgcggttcc agcgcgtgac gccggcattg gcatcgtcca caaagtcatc
     1801 gcgcagccgc atattcagcg ccgacagcgc cggcacgtcc tccctggcaa tctctccgtc
     1861 tttcaggcgc agcaccggat aagtgtcgtt gcccagcaca tgatcgtcgg tcagccgatc
     1921 ctcatgatag cgccccttca gcccggcatt gaacgcgttc gccgcattgg tgctgatctc
     1981 gttgccgaac aggtccagtg tcagcgagaa atgcagattg gccttgcgct gcaacgtcgg
     2041 cagatcgatc acgcccagcg cccgcacccg ttccacatcc gccggatcat cgatcccagc
     2101 ctcacgcatc gcatcacacg tgcgctgcac gatccgctgc acgccagtct cgccaacgaa
     2161 catatggtgc gcttcctcgg tcagcatgaa gcggcaggtc cgcgacagcg ggtcgaaccc
     2221 cgactgcgcc agcgccgcca actgcatctt gccgtcacgg tcggtgaaga acgtgaacat
     2281 gaagaacgac agccagtcag gcgtcgcctc attgaacgca cccagcatac gcggactgtc
     2341 ctggtcaccc gagcggcgcc gcaacagttc ctccgcctcc tcccgcccgt cacggccgaa
     2401 atatttttgc agcagataga ccatcgccca aaggtgccgg ccttcctcga cattcacctg
     2461 gaacaggttg cgcatgtcgt attgcgaagg cgcagttact ccgagatgcc gctgctgctc
     2521 aacggatgcc ggttcagtat cgccctggat caccagcaac cggcgcagca tcgcgcgata
     2581 ttcgcctggc acttcctgcc ataccggctc gcccttgtgc gcgccaaagc cgatcttgcg
     2641 gtcttctacc gcgggcgcca gcagaacgcc ccagcgatat tccggcatct tcacatagcc
     2701 gaacttcgcc cacccttccg atccgacgcc gatcgccgtg cgtagataca ccagccgatc
     2761 ctggaaccct tccgggccca tctgccgcca ccagtcgata tagccgggat gccaggtctc
     2821 cagcgcacgc cgaacgcgcg catcgtcggc caggccaacg ttgttcggga tcagcccgtc
     2881 atagtcgatg gtcgttccct cacccatcac acgctcctcc gccatccgtc gcggccaacc
     2941 cacgacccgc ccatcgaacg tacctccgac atccgtatcg tcatggccgg gcttgacccg
     3001 gccatcaaac gcgctccgtg tcatagtttg gccgcagccc gctgccgtag cgcttcaacg
     3061 atccttccgc gccaactgcg ctcggccgct ggaacaccca gttctgccag gccgtcaggc
     3121 ggccaaaaat ccgcgactcc atcgtctcag ggccggcaaa gcgcaggttc gcttccatcg
     3181 cggtcagcgc atccggcgag aatgatgcac gctcctccag catcaggcga acttcatcgc
     3241 tccagtccgt gtcatcatag gcaagcgtca ccagccccgc tgcttcagcg tcctccgctt
     3301 cgagccgttc cccaacgcac tcccgtgctc gcgcaaagct gtccggttcg ccgaggaacc
     3361 gcgtcgccac acgcgtaagc ccgttgccca tcgggtaagc gccgaagttc agcgccgaca
     3421 gcgcgattgt cgccggtcct cggttatcgc tgctgcgcgt cccggcgaac atcactgatc
     3481 ggtccgccgc gaacagcaac tccgccagcg tgcccgcgaa gcagctcccc ggctcgatca
     3541 gcgctatcag gctgcgcgac gtcatatcca gccgcttcaa cacgcgtttc cagtacagcc
     3601 ggatctcgcg caccagccag tcgtccttgc gcgcctccaa ctcctcgtcg gccgccaaca
     3661 cggccgccgg atccccctcc gaccgcagca cgatcagacc aagcgtcggc tcgttcaggc
     3721 gcaggtgcag gatcgcgtca tccagctcac gcgccagcag caacgcccag tcaaggccag
     3781 acacctcccc aggtccgcgc accgtgagct cggcccgccg ctgcccgcga tcgaacgtcg
     3841 cacgcacatg tgtatataca ttcccatcat cactcaacgt gcgctgcaac ggcgccagca
     3901 cgacaccctt ggcatcgacc ggacgatccg acttctccgc caatgccaga gcgcgttcgc
     3961 gcagcttcgc ctcccatgac gatggcggca cgacctcatc caccaggcgc caatccaccg
     4021 cgcgacgccc gcgcacgccc tcctcggtgg agcaaaacac atcggcccga tcgcgccgca
     4081 ccatccgctt gtcggtcagt cgcgtcagac ccccagttcc cggcaacacc gcgagcagcg
     4141 gcagttccgg cagcgccacg cccgaccgtc gatcatctac cagcatgatg tgctcggccg
     4201 ccatcgccag ttcatagccg ccacccgccg cgctgccgct caccgccgcc agccagcgct
     4261 ggccggaatt gtcgcaggca tcctcgattg ccagccgcgt ctcgttggtg aacttgcaga
     4321 aattcacctt atggccgtgc gtcgccttgg ccagcatgcg gatattggca cctgcggaga
     4381 acaccccttc cttgccggag cgcaggatca ccgtgcgcac ctcgggatgc tcgaagcgca
     4441 gccgctgcac cgcatcggcc agctcgatgt ccacgcccag atcgtacgaa ttcatcttca
     4501 gttcgcagcc ctcgaacagg ccgccatccg gatcaacgtc catcgtcaat gtcgcgatcg
     4561 gcccgtcgaa cgccaggcgc caatggcgat aacggctcgg atcgatttga aagtcgatca
     4621 tgcggttgct ttcagcaata cattgcgaac gaaaggcacg atcgcatcca gggtcgcctg
     4681 ccgcgcctcg agatgcggtg aatgctgcgc gccgtcgatc acccgcgtct ccacatcgca
     4741 gcgcgcggcg cgctccagca cacgcagctg ctcgtcggag ccatacggat cctccgcgcc
     4801 ttgcagcgcc agtatcggca cacggatact ggccacctgg tcagtgatgt caaagtcgcg
     4861 gaagcgcggg tcaagccagg agtcgttcca cccacggaac gccacctcga catcccggtg
     4921 atagcgggcc agccgcgccc tcagatcccc ctcctcgtag ctccgcctga tggcaccgat
     4981 cgccgcaacg ttgaggtcct cgacgaagaa atgcgccgca agcatcacca cgccaagcac
     5041 ccgggcatca tgcacggcac cggcatagat cgccgcaatc gatccaccat cggagtgccc
     5101 gagaagtatc ccgcgccgca ccccggcagc gtccagcacg tgcggcaata tcgacagcgc
     5161 ctcatcatgc atatacgtca ccggccgcgg cagcgtcacc ggatccgatc cgccataacc
     5221 aaagcgcgag taggcgaaca cgccacagtc ggtcgccgcc accagcgcct cggggaaatc
     5281 ccgccacagt gtgacgcagc ccaggccttc gtgcagcaac accagcgtcg gcgcgacctc
     5341 cggacccggt ccccaccatt tggtctcaag cgaacggccg tcgatgatca ttgcaaacgg
     5401 aaccgttgga tcttgccggt cgccgtcttc ggcaggccat cgacgaattc gatccagcgc
     5461 gggtatttcc acaccccgat ccgctctttg acatgcgtct tcagcagctc tttcaccgtc
     5521 tcggcatcct gctgcctcgc ggcatcctgc agcacgacga acgccttcgg cttggtcaga
     5581 ccatcatggt cctgcgcacc gatcaccgcg gactccaaca ccgccggatg cgacaccagc
     5641 gcctcttcca cctcgaacgg cgatacccaa acgccgctca ccttcagcat ttcgtcggta
     5701 cgcccgcgaa accggaaata accctcatga tcgcgaatat aggtatcgcc ggtgtgcgtc
     5761 cactcaccgc ggaacgtgcg tcgcgtcttg ccgcgctggt tccagtagcc gtcggccgca
     5821 gatggaccac gcaccaccag ttcgccttcc tcaccgttcg gcacccggcc gccgtgctca
     5881 tccaggatca gcgcctcgta acccgggacc ggcttgcccg atgtgccata tcgaacatca
     5941 tcagcacggt tcgacaggaa gatatgcagc atctcggtcg aaccgatgcc gtccaggatg
     6001 tcgacaccca ccatatccga ccagcgccgt ccgatatcgg cgggcaacgc ctcaccggct
     6061 gaaatgcaac gccgcagacg ctgcgagccc gccttcggcc cgatatgggg attggcaagc
     6121 atcgcggcaa acagcgtcgg cacgcccgca aagatggtcg gctgatgccg cgccatcgtc
     6181 tccagcaccg catccggcgt cggccgctct ggcagaagca cggcagacgc tccgatggac
     6241 atcggaaacg tcagcgaatt gcccagccca taggcgtgga atgccttcgc cgccgagaac
     6301 atcacgtcgt ccgcgcggat ctgcagcacc tgtgcgccat agctgtcagc ggtcgcgcgc
     6361 aggctgccat gcacgtgccg cacccccttc ggcgcacccg tcgagcccga tgaataaagc
     6421 cagaacgcga cctcatctgc cggcgcatcc actgtcgccg tcgccggatc gccaccctcg
     6481 atgaacgccc cgaaccccac cgcgcgcgca tcatccactg ccgccgcact gccatccggt
     6541 gccgcaacaa tgatccgccg cagttccggc acgctgtgca ataccggcag cagcggttcc
     6601 accagcggtg cggaaatcac cagccccgtg gcccggctat ccgccaggat gtacccaacc
     6661 atctcatgtg tcagcagcgt gttgatcggc accggcacca cgccggcccg catggcaccc
     6721 cagaacgcca cgggaaaatc gatggtgtcg agcagcagca acaccacccg ctgctcgcgc
     6781 gcgatgcccg ctccgcgcag cgcacccgcg aagcgtcgcg tcgacacttc caactcgccg
     6841 tagctaagtg accgccacgg atcgcggcac atcagcgatc cgccacgtcc ctcactgaca
     6901 tgacgatcga cgaagtactc gaccgcgttc cccgcgcctg agagcatcca ccatcacctc
     6961 cgcgtggagt ataaccccgc gcctggaggg ctggcgatgg ctccagagca ttggcaatgc
     7021 gaatgccata cggtcagccc cctcggctgg ccagaatggc ccagatacct ccgcacgaga
     7081 tcgcgacacc atagggcagc gaaccgcgcc gcgcgatacg ccaccactcg gctgtcaccg
     7141 cacggcgcag caacggggca cctcgcggcg gcgcgtgcgg cgccgtacgg atcgccgcac
     7201 gcagcacgag atgcaacaac gccagaacgc ccccggcgag agcggtgatc atgacgaacc
     7261 agtagatcga tccgatcggc agcccaagca caatggccgc cgcgagcttc acgtcgccgc
     7321 cgcccatgac accccggctg tgcaaaacga cgagcaatgc aagagacagc agagcagcaa
     7381 cgatcgaggc ggcaacgctg ggcaggccag ccatgacgcg cgtcgcaagc ccgagcaccg
     7441 ccacccaaac gacaatgcca tcaggaatga tgcgggccac gatatccgcc agcgccgcgt
     7501 acaccagcag agccacaacc gccgccgtca caaggttgac tgaccacacc tcgatcgcac
     7561 ctggtctgac cacttatcac atgctcgcac cgactgatgg cagcgcacca gtcgcaagaa
     7621 attggaaccg caaatcacgc aaaaggctac ggcgtggagt gaccccccga actgagacac
     7681 gacaaagaca cactctgcga ggagaggaga acgagtgtgc gtcgtttcct tgtctcacct
     7741 taggggcact ccaaagccac tttttgcgtg ttttgcggtt ccattttcct tcggacctgc
     7801 tcacacccgc caagcctgcg ccccttgctt ggtgaagcca cagatgcgcg tctgcccagg
     7861 ctcccgctcg agcacccgca gcagacccgg tcgccgctcc ggcggtacca ggaagaacag
     7921 gaatccccca cctcccgcgc cagatacctt gccgctccat gcgccggatt ccatcgcgag
     7981 tgcgtgcacc cgatcgatct ccccgttgga gatcgcactt gcagtgcgct tcttcgcctc
     8041 ccagcttcgc cccagcaccg aggcaaacgc ttgcagatca ttgtgcagca gcgcgtcctt
     8101 catcacatag gtgtcacgcc gcatctgatg catcgcctcc agcgaggccg catgtccact
     8161 ccgcaggctg gccgattgct cggtgatgat cttcgccgat tcgcgcgaga tccccgtcga
     8221 gtacagcacc agcatcgcct ccagctcggt cagcgtcgcc gaacggatcc ggagcgggtt
     8281 cacgaccgtc tgctcgcctg caaactccat gaaattaatg ccgccaaacg ttgcagcgta
     8341 ctggtcctgc cgcccgcccg cctgccccac atccacgcgc tcgatctggt aggccaggtt
     8401 cgcaacgtca tactcgccga gcggcaacgc caacagttcc cgatacgcct cgatgatcgc
     8461 cacgacgagc gttgacgacg aaccaagccc ggagcccggc agcgcatcgg agctcgtcat
     8521 caccgtcacc ggcagacatg ctccaccaag atactgccca acgatccgcc gatgcaccgc
     8581 tgcgtgcatt gcaagcagac caggcggcgc gcccaccgca tcgccgtcaa attcccactc
     8641 ctgctcatgg tcgagtgcca cgaaccgcac cttctgatca tcgcgcagct tgatcgagca
     8701 gtacgcaaac atcccgatcg tcgcgttcag cacgcaacca ccaaacgtat ccgcatagaa
     8761 ggcaaggtca gttccgccac ccgcaaagct gatgcgcaga ggcgcccgcg cgcgcacccg
     8821 cgtcacatgc gccgatcggt ccagcagagc ccgcacgttc accggtgtcg ggcggaccgg
     8881 gcgatccagc agctcactca tggcccgcgt cccgttccga caggattggc ggcctgatcg
     8941 gccaggcaat cgcataggcc ggatcgtccc accggatcac ggccgccgcc gcggggtgat
     9001 gtgccgccgc catctgatag aacacctcgg agtttgccag cagtgtctgg aacccgtggg
     9061 caaaccccgc tggtatatac acagcaagcc cgttctgctc gctgagctcc acagcgtgcc
     9121 atcgtccgga ctcgcggtgc agcagcacat ccagcaccac cccgcgcgtg caacgaacca
     9181 gtttcacctc ctcataagga ggacgctggt agtgcatgcc gcgcaatgtg ccacgccgca
     9241 cattgaacga ggtgctgcat tgcacgaacg atccggccag cccgtgcgca gcaaactcat
     9301 cgctgcagaa ggttcgcgcg aaatagcccc gcgcgtccgc atgccgctcc ggtgcgacga
     9361 cgaaagcgcc cggcaacggc agcggatcga actgcatcag agcacctgcg cgcgagggat
     9421 cggcacgacg aaccggccgc cccagtcgcg tatcgcctgc atttgcccga cgatctcgtc
     9481 gcgcaggttc cagggcagga tcagtacata atccggccgg atctgaagga tgcgctccgg
     9541 tgaaaatacc gggatctgcg agccaggcag aaaccggccc tgcttatgcg gactgcggtc
     9601 aacggtgaac gccagcagct ccgggccaac cccacaataa ttgagcaacg tattgccctt
     9661 ggccggcgca ccataaccca cgaccagctc tcccccccgc cgcgccttga tgaggaattc
     9721 cagcagcgcg cacttcgcgt cgatcaccgc ttcgccaaag cactcatagg tctcgattcg
     9781 atcgagcccc gcagcacgct cctccgccag caccgcgtgg aaccgcgccg tgcgctgcct
     9841 gttcgccccc acatggcacg catgcacgcg cagcgagccg ccatgcgtcg gtaaccgatc
     9901 gacatcaaac acatacagcc cgtgacgtgc gaacagacga tcgaccacca gcagcgaaaa
     9961 atacgagaaa tgctcgtggt agatcgtatc gaactgcgtc tccgcgatca gccgctgcag
    10021 atgcgggaat tccgccgtaa cgatgccgga cgtcttcagc agcaccggaa aacccgcaac
    10081 gaaaccattc aggtctggca catgcgccaa cacgttgttg gcacagatca gatcagcctt
    10141 gccgcgttcc gcaagccggc atgctgtctc gatatcgaag aaggcaatct cggtcgcaat
    10201 ccctttgccg atcgcaacgg cggcaacatt cgccgccggc tcgacgccca ggacgcccac
    10261 cccgcgttgg cggaagtact gcagcaggta gccgtcattg ctcgcgacct cgacaaccaa
    10321 cgactgcgca tcgagccgca gttcgtccgt catacgcttg gcatatgcat ccgcatgccg
    10381 cagccaggca tccgagtagg acgaaaaata gagatactca ccgaatatct gctcagccga
    10441 tacgacttcc tcgacctgcg ccagcagaca ttcctcgcag acataagcgc acagcggata
    10501 atgcggctca ggcaaccgaa gctgctccgc ggtcaggcag gcattcgaca gcggtgccct
    10561 gccgagatcc acgaacttcc tgcgtagcgg cgccccgcag aaccggcagg tcatgccggt
    10621 gcgcatccgc aatacttgac gatctgcgct cccgtcagcg caagcgccgg ctcgcccttg
    10681 gcctgtcggc ggtaccagtc gaccgtccag cgcagtgcct cctccagcgg cagccgcggc
    10741 gtccaaccca gccgcatacg tgcctttgag gcatcaacct tcagcgtttc cgtctcatgc
    10801 ggcccatcgc cggagacaat ttcccagctc gcgtgatcac cccaacgctc gatgagattg
    10861 gacgcaatcc agccgaccgg ccgtgcatcc tcatcgggcg gcccgaaatt ccatgcgtcg
    10921 ccgaaccgcg cgggattctc cgccaatcgt tcagccagca acagatagcc gcacaagggc
    10981 tccagcacat gctgccatgg ccgtacggca tgcggattgc ggattgcgat cgacgcgccg
    11041 gcagtcagcg cacgaataca gtccggcagc agccgatcct ccgcccagtc accgccgcca
    11101 atcacgttgc ctgcgcgcgc cgtggcaatg gccgggtaga acgacctgcg atacgacgat
    11161 gcaaccagct cggcacagcc tttcgaggca ctgtacggtt cgcgcccgcc aactgcatcg
    11221 atttcacggt atggccacgg ccactcgcga tcctcgtagc atttgtcgct tgtcaccacg
    11281 accgtgcaac gaactgacgg cgtcgcgcgg atggcctcca gcacatgaac ggtacccatc
    11341 acattggtcg cgaacgtctc caccggctcg cgatacgatc ggcgcacgag agcctgcgcg
    11401 gcgagatgga acacgacctc gggccgccac cgtgccagtg ccgcagccac tgtcgcccga
    11461 tcgcgaatgt cgccgatgtg cgtctcgacc tgcgcatcca ggcccgccag cgcgaacagg
    11521 ctcggctccg tcggtggcgg caacgacagg ccaacgacgc ttgcccccat ccgatgcagc
    11581 cagacgctaa gccaggatcc cttgaaaccg gtatgaccga cgacaaggac gcgccgtccc
    11641 tgccagaagt cttggcgcgg attgagcaga ggttgcacgg cgcgcaccag agcctcccga
    11701 cctgcgatgg caggcgggca gtggaacccg gcggcgtaaa gaaatggtta gcggaacccg
    11761 cgaagtccta tcgtgaggag cgagccagtt ccgacgcctg gtcgcgcgcc ctttgcatcc
    11821 ctcgcaaaat cgcctccgcg tccagctgcg actggtgctg ccgccacccc tcatcgtgcc
    11881 gaaccagatc ggcctcggat ccagacgggc tcattccgca agcagaaagg caggcaaatg
    11941 tgatcagggc agcaacaagg cggatcacgg gcaattcact cctctgatcg acaatttgta
    12001 gcgaccagcc gtcgcgggcg ccagaattaa agcgcgagtg ccaatcaatc tgcaacggcg
    12061 agcgccggtg gccgccgcaa cagccagaac gccacgccgc cacaaacata aaagaagatc
    12121 gaaagtgcaa acgccacatg ataattgccg gtcatatcaa agctccaacc ggccagccac
    12181 gagcccgccg ccgaacccac cccagtgccg atggtgatcg cgccaaggat ggtacccagc
    12241 cgaggcccgg gaaacagatc cgccgtcttc gccgtgatcg ccggtccgcg cgcgccccac
    12301 gtcaggccaa agaaacacga atgtagccaa agcaacagat gttgatcagg agagctgatc
    12361 aacatcgcgc acacaacacc aaaaatcgag gttccgtaag tcactacggc agaaagttca
    12421 cgtccaatgt aatctgacat ccagccggtg acgatgacgc ccggcagcga caggaacgcg
    12481 ccaagcccca gcacctcggc ggcatagagt ttgtcgaatc cgatggtcac cgcaaacgca
    12541 agctggtgca gcgccaccag gaacgaaccc agtccggtga acacgtagac caggaagagc
    12601 agccagaaat gcggtgtgcg cgccgccagg cgcagcgtcc agccagacgg tagtccagcc
    12661 tggctggacg ctgtcggatt gtccgcatcc gaactccgga acatcgcggc cagcggcagc
    12721 accgccaccg ccatgaatcc cccctgcgcg agatacgcgc cgcgccagcc cagtcccgca
    12781 atcagtacct gtgaaagcgg cgcggaaaac gcgcgtgcaa aaccattggc cgactgcacg
    12841 accgccaccg ccgtgccgcg atgccttaca aagcgccgcg acaggatcgg cacgaacacc
    12901 accagaccca ggcagttggc ccccagtgtc atcagcacgc cgtacagcag gatgacctgc
    12961 cagagcgagt gcacctgcga gcttgcgagc agtccgaccg ccagcagagc gccgccgata
    13021 agcaccagcc gcttcggtcc cagccggtcg accaggacgc ccaccagcgg cgaggttgcg
    13081 ccggtgatca tctgcgacac cgaataggcg atcgagcttt ccgcccgggt ccatccgaac
    13141 gcctcgatga aggcgatcag gaacaccgtg taggaatgca tgcacgcagc accgacactg
    13201 aacgtgacga agcatgctgc aagcaacagc caggcctggc gttgcccggc actcaggcgc
    13261 atggattggt tgcttcggtt cgatgcgcca cgacacggcg agtgtgcccg ccgtcggcct
    13321 cggcctcaac ccctgcgtgg cctgatcagc acgatcaccg cgccggcaaa ctgcagagca
    13381 atgcaaagca gcagcgccgc gctgtatccg ccagttgcgt cgtgcgccag acccagcagc
    13441 gtcggaccga acgcgagcag cgtatatccc accatcgaga tcagcccgat cacgaggcca
    13501 aacgattgtg ccgcaaactc tcgctgcacg atcaacgccg gcagcgtgat cacattgccc
    13561 accgagacgc cgaacacgat gctgccggca taaagtgcga caggttgttg cggcagcaac
    13621 aacatcagca ccaacgccgc cgcctgtgag gcgaagctcg ccgccgacgc cacgcgctga
    13681 ttgagccgat cgatcagcgg tgccatcgcc agccgcccga ccgctgccgc caatgctgtt
    13741 gcggcaaccg cattggccgc gccctcattg ccgagttgcg gccgcaggaa ggccagttgg
    13801 tgcacgatga aaccaacctg cgctgccagt gccaacgcga acggcagtgc aatgctccac
    13861 acgcgaacgc tgcgcagcgc ctcaccctgc gtggtgaatg ccggcaagtg ccctgtctcc
    13921 cggcgcacgc ttggccgcgg cgaggcactc tgctcgatcg acgcaacacg ccccacgccc
    13981 accaggatca atggcaacag gatcgccagc ccgcacagca ccagccacgt cacgcccggc
    14041 cccagtccga acatccgcac cgaacgcgcc aggattggcg tgacgatgaa cccgccgccg
    14101 cttgcgccat tcagcgcaag actcagagcc aggccgcgtc gcctgtcgaa ccagtgcgcg
    14161 agagtcgtgg caatcgccgt ggtggtcgtg gcagaccacc caaccgccat cacgagccct
    14221 gccgcataaa gctgccacgg cgcggtcacc cgaccgagcc atatcgcgcc gacaccaagt
    14281 acgactgcgc caaccgccag caacagacgt ggccccagca acgccattgc ccgatgcacg
    14341 taagccagca tcaggccgcc cgccagatag tagaccgtcg tcgccgacgc gatcagcgac
    14401 gacggccagc cgcgcgtcgc atgcaggtcg gcgaaataca ccgattgacc ataaaacccg
    14461 aaaccccagg caaacacggc cgtcgcaaag cacgccaata ccaccggcca gccttgacgg
    14521 aattcgccct gtgcggggac cactgcaata tcgctcatgt gaggaaactg agatgccacc
    14581 cggcgccaca caaacgattc ttcatggcag cccggaagga gaaaatctaa actgaagcat
    14641 gtccctcccg ccgctcaacg cgctgcgtgc cttcgaggcc accgcgcgcc atctcagcat
    14701 gaaggaagct gccaccgaac tctcggtgac acctggtgcc gtcagccagc tcgtgcgcgg
    14761 ccttgagcag cggctcggca cgcaactgtt ccgccgcagc aaccgcgcgc tggtgctcac
    14821 cgagtccggc cagagctact tcgtcccgat ccgtcacgcc ttccgccaga tcaccgaggc
    14881 cacccgccgg cttcaggcca cccccaatgc cggtgtcctc accgtcagcg cgccaccagc
    14941 cttcgccgaa tcctggctgg tgccgcgcct cggcacattc cgcacacacc atcccgacat
    15001 cgaactgaac attgccacaa cgcgccgtct ggcaaacttc gtcgcggacg gcgtcgacgt
    15061 cgcgatccgt cacggcctcg gcacgtatcc cggcctgcgc tgcgaccgga tcgcgacgat
    15121 cgccatgatc accgtgtgca gtccgcgctt cctcgccgca ttgccgcgac ggccacggac
    15181 gcccgccgat ctgctgcaca tcagcctgct gcacgaagcc gagcggcagg aatgggcgct
    15241 gtggttccag gcgcaaggcg tcaccgatgt tggtcacacc gcctccagcg gcatcagctt
    15301 cgacgaccaa atgttgctga tccgtgccgc tgcatccgat cagggcgttg cactggtcac
    15361 cgaaacgctc gcccggcgcg agctcgataa cggcaacctc gtgcgcgtgc tggatgtcgc
    15421 ctggccgcaa gagttttcct attggctggt gtgcccacgc gccactgccg aagtgccaaa
    15481 gatcgtcgcc ttccgcgact ggctgatcag ccagcgcgcg cgtcaagcca ggcgagcaac
    15541 gccgaaccga acgccctgag gctcttgtaa tgcatcgtag cctccggcaa cgcgcgcacc
    15601 gcttcggcca accgcgccgc atgctccggc cgcgtgatcg cgcgcgccag ctgatagctg
    15661 tgcacccgga tgccgaacag gatcgcaccg gactgcggca ggcggcgcag cgtctgtcgt
    15721 tccacccgta gatacagcga gtcgccggcg ttctccggcg tgatccgcgc attggtcgct
    15781 tcacgccact tccccgtcgg ctgatacaac gccggatcgt ccagcactga ccagttcagc
    15841 cgcgaggcga tatgtcccac cttcaccctc gccatgaacc gatcaaccgg tgccgcgagc
    15901 cgttccgcgt aatgcggcac cggcccatgg accgacgtca acggcttgcc gagcttctca
    15961 ttcagccgcc accggcttgg aaagcacagc actgcggccg tgaagcgcgg gccatcagcg
    16021 ccgttctgga tgatgcaaag atcctcctgc accagccggc cagccacctc cagcggatca
    16081 cacggcgccg ccgcaagctc ccagctttct cctgtcagcg cattgcgcac aacgtcgccg
    16141 tcacgcgcga accactgtgg tgcgtgctcg cacagattgg cgacgacttc gcgcagcgct
    16201 tcgccacgcg ccgcctctga tccaggcagc gccccaaaca catcgccgtg ccgcacagcc
    16261 agcaactctc gccgctcccg catctcatcg acatagcgct catcgatctc gaaccacgcg
    16321 ctctccggca cagtcgtcag cgacatcgac atgcggtacg gcccgctctc gaacggcagg
    16381 taaatcgctt ccggcagcgg catttgcatt gcgcaatctt gccagaggcc agccacctcc
    16441 gtcgagcagt accgtgcgac ttgacgcaag ccccgtcagc gcgacactgc cgcaccgatc
    16501 cacagaaggg ggaaacgaaa ccatgccaga cgtccgcatc atggccgaga aattcggctt
    16561 ccccgaaggt ccggtggcga tgcccgatgg cgcggtgatc ctcaccgaga tcgccggccg
    16621 ctgctgcacc cgcatcggcc ccgatggctc ccgttccacc atcggcaatg cgggcggtgg
    16681 tcccaatggc ctcgcactcg gcccagacgg tgcgctctat tgctgcaaca atggcggcgg
    16741 caaataccca tccggccatt ggatggcgat cggtcccgcc gacgactacg acggtggctg
    16801 catccagcgc atcgatccga agaccggcga cacgcgcgtg ctctacacgc acgtcaacgg
    16861 ccacaagctc tccgcaccga acgacctggt gttcgacacc aatggtggct tttacttcac
    16921 cgatctcggc aagcgtttcg cccgtacgcg cgatcacggc ggcctctatt acgccttgcc
    16981 tgacggctcg aaagttgtcg aggtcgccta tcctgtcctc agccccaacg gctgtggcct
    17041 gtcgcctgat ggcaaggtgc tctacgtcgc cgatacggaa cccgcgcgcc tttgggcatt
    17101 cgacatcacc acgccgggcg tggtgaagaa gcaaggctgg ccatcaccgc acggcggcag
    17161 actgatcgcc ggcctcggtg gcttccagcg cttcgacagc ctggcagtgc aggccaacgg
    17221 tgatgtcgcc gtcgcaacgc tgcacgcggg ctgcatcacc gtcatctcgc cggacggcaa
    17281 gatcgtacgc caggtgacca tgcccgacga attccccacc aatatctgct ttggcggcgc
    17341 tgatatgcgc actgcttacg ttacgctgtc agccaccggt cggctcggtt cattgcaatg
    17401 gccagaaccc ggactgaagc tgaacttcaa ctgacctgat ggaacctccg gcgacgtccc
    17461 gcgttgacgt cgcctctctc catggaggct cctcaatgag cgacaggttc gaaggtacgg
    17521 cccgcgagct cggcggccgc gtgcaacaga aggtgggcga attcgccggc gatgcgaaga
    17581 cgcaggcgag cggcttgtac aaccaggccg ccggacaggc cgagcagcag gtcgcaaagc
    17641 tgagcgacgt catcaaggac caaccgatca cctccgcatt ggtcgctatc ggtatcggtt
    17701 atctgctggg acgattcacc gcctgaacaa aagttgtctg gaatgaatgg ccccgctgcc
    17761 gcatctcaac gcggcagtag gggagaagcc atgccgtcga acgcccgcaa gtcgcgcctg
    17821 agcgaggggt cgcccaatcc tcgcggcgca acgtgggatg ggctcggcgt aaacttcgcc
    17881 ctcttctcgg caaacgccac gaaagtcgag ctatgcctgt tcgatgacgc cggccgcacc
    17941 gagctggagc gcatcgagct ccccgaatac accaatgaag tgtggcacgg ctatctgccg
    18001 gatgcgcgcc ccggtaccat ctacggctat cgcgtccacg gccaacacga ccctcgcaac
    18061 ggtcatcgct tcaatcccaa caagctcgtg ctcgatccct acgcaaaggc gtttgtcggc
    18121 aatcttcgct gggacaatgc attgttcggc tacacgatcg ggtcgaagga cgaagatctc
    18181 agcctcgatc ggcgcgacaa tgcgcgcttc atgccgaaat gccgggtgat agatccggcg
    18241 ttcacctggg gccgggaacg ccgtcccgac gtgccctggg accgcaccat catctacgag
    18301 acgcatgtcc gcggccacac caaactgcat ccggcggtgc cggaacgcct gcgcggtacg
    18361 ttttccggcc tcggccgcaa ggaggttgtc gaatacatcc gctccctcgg cgtcacctcg
    18421 gtcgaactgc tgccgataca cgccttcgtg aacgatcggc atctgctgga aaggggcctc
    18481 accaactact ggggctacaa ttccatcggc ttcttcgcac ccgacccacg ctattccgcg
    18541 gtgcccgcct tcgtgttctc cgaattcaag gaaatggtcg cgcgcctgca tgatgccgga
    18601 ctcgaggtga tcctcgacgt cgtctacaac cacaccgccg aaggcaacga acgaggcccc
    18661 actctctcgt tccgaggcat cgataacgca tcttattatc ggctgctgcc cgacaatctt
    18721 cgctattaca tcaacgatac cggcaccggg aacacgctga acctgtcgca tcccagcgtg
    18781 ttgcagatgg tcaccgactc gctgcgctac tgggtgaccg aaatgcacgt ggacggcttc
    18841 cgcttcgatc tcggcaccat cctcgcgcgc gaagaccacg gcttcgtcga gacacatggc
    18901 ttcctgcaaa gttgccagca ggatccggta ttgtcgcagg tgaaactgat cgccgaaccc
    18961 tgggacattg gaccgggcgg ctatcaggtc ggcggctttt cgccaggctg ggccgagtgg
    19021 aacgacaaat tccgcgacac cgtccgcgcc ttctggaagg gcgatgacgg caagctcccg
    19081 gaactcgcca cgcgcctcgc cgcctccgcc gatgccttca atcgccgcgg acgcaaaccg
    19141 tggtccagcg tgaatttcat caccgcgcat gacggcttca cgctgcacga cctcgtctcc
    19201 tacaacgaca aacacaacga agcgaatggc gagaacaacc aggacggtca ttccgacaac
    19261 ctgtcgtgga atcacggcgt cgaaggtgaa acagacgatc cggacatcaa ggcgctgcgc
    19321 tgtcgccaga tgcgcaacct gctcgccacg ttgctgctgt cgcagggcac gccgatgctg
    19381 ctcgccggtg acgaattcgg ccgcacgcaa aagggcaaca acaacgccta cgcccaggac
    19441 aatgagataa gctggatcga ctggaacatc ggcgaaagcg gcggcgcact catcgagttc
    19501 gtccgccgcg cgatcgcggt gcgtaacgcc ttcccaatcc tgcgccgctc gcgcttcctc
    19561 accggcgaat acaacgccga attcgacgtg aaggacgtgc gctggctgac gcctgtggcc
    19621 accgatatgg aggaagagca gtggcgtgac ggcaatgcgc gctgcttcgg catgctgatg
    19681 gacggccgcg cacaggccac cggtatcaaa cagccatcat tggacgcgac cgtactggtg
    19741 gtgctgaatg cccaccacga cgtcgtgaat ttcacgctgc ccgaggtcct cggcggacag
    19801 gcatggcgct gcctgctcga taccaatgtc cccgacctca ccgcagcgcc gcgcatcacc
    19861 agcggcaacg aataccaggt caccggacgc tcattggtgc tgttcgcaat gcaaccggat
    19921 aatgcgcaat ccgttgcact cacccgtgcc cgcgaggcac tgcgccaggt cgccgaaaat
    19981 ccccttgccg atctgctcgt ggaggaaacg gccaactgat gtccgacccg cccaaaccgc
    20041 ccggttcgcc gccagacgaa gacgaccccg atgcccccgt gccaatcgag gatccgccgg
    20101 cgccgatccc cgtcccgccc gatctgcccc ccgaaccgct ccgcgcgctc aggctcgctt
    20161 cacattccag aacagcggcc cgttgcattc cagcttcccc accaggctct tccgatacgc
    20221 cgtcggaatc tggaactgcc ccagcgttat cgtcgccgcc tgcgcgtaat tctccgcctg
    20281 gatcgccgcc gcaatcttct tccgcgaatc atcgtcggtc gcgtccagcc actcctgtgt
    20341 cagctcttcg actttcgtgt cgtcatacca gccgaaccat ccggccgtcc cctgcccgcg
    20401 gaacggcgcc gtcagcaccg gattgaggat gaacccgccg gtgaaccagg tatggaacac
    20461 tgaccagccg cccttctcca ccggctcctt gctggcgcgc cgctgcacaa cggtgcccca
    20521 gtcggtatcc gccagctccg cattgatgcc tagcttcttg aacatgtcgt aggtgatgtc
    20581 gccgaacggc ccgattgtcg ggaagtccgt cgggttgatg atcaccgcct tggcgccgtt
    20641 gtaatcggcc tccttgatca tcttcttcgc ggcttccagg tcgccctgct gcaccggcag
    20701 gttcacctcg gtgccataca tcgtcccaca cggatactgc gaacggcaga tgcgccaggc
    20761 cgacgtatct tcacccatga tcgcgcgcat gtagtcggcc tggttcaccg cagccagcac
    20821 cgcacgccgc agccgtacat cattgaacgg cggctgtatg gtattgaacc gcatgccgcc
    20881 aacgtaaccc tgcgggttcg atggcccgag gacaatttcg ccattcttcc gcagcaacgg
    20941 cacaaggtcc tcggctgcct gctcgtacca gtccacttcc ccggtctgca gtgcggccga
    21001 cgccgtggac gcatccggaa tcaccttcca ttcgatgcgc gggaactcca cgaccttgcc
    21061 acccgacgtc cactccggct tctcttgccg aggcacgtat ccgtcgaact tctcccacgc
    21121 cgcgccactg cccgcgacga aatcgctctt gatgaagcgg aacggccccg aaccgatcat
    21181 ctccgtcacg cccttgaacg gatcggtctt cgccagtcgc tccggcatga tgaaggacgg
    21241 ctgcatcgcc atgccggtga tcagcagcgg gaatggccgc ttcagcgtga tcttcaccgt
    21301 cttgtcgtca gcggtccccc aactctccac cgactttgcc gcggtctgcc cgaacgtatc
    21361 ccgcgcggcc caccgcgcca ggctcgcggc acagtcgcgt gccagcaccg gctcgccgtc
    21421 gtggaacttc aacccgtcgc gcagcttgat caggcaggtg cggccgtcgt ccgaaaccgt
    21481 gtagccctcc gccatttgtg gtttcggttg taactgtgcg ttcacgccaa acagcgtgtc
    21541 gaacaccatc cagccatggt tgattgtcac cgccgccgtg gtgatgatcg ggtccagcac
    21601 cgtcaggttc gcctgcggca caaatttcaa cacccgcgca ttcgccggct gagcgatgga
    21661 gaatcgcggc aacgccgctg ccgcgctggc ggcgagccct gtccgcacga atgtacgccg
    21721 tttcatgaag cccccgcttg cttattgcac cgcagcatgt cgagtttcac tacaagccgc
    21781 aagcaagatc gccacgaacg attacctgcg acgcagccgc cgcctgtcat tgcgaggccc
    21841 ccggcgaagg ccggggaacg aagcaatctc gaacgggttt cgcctgatcc tcccgaattc
    21901 cttcatcggt tcgctccctt cccctccttc ctccaccaca ccttcgtatt cacgaacgta
    21961 accacggcat attttgccgt tctgttcacg ttttgtcagc gtttcctcgc tagattgccg
    22021 cccacagacc cgcccgagcc gcgctctgcg ggaaggatgc atggcgaaaa actcgcaagg
    22081 gccaagagcc ctgcgacgat gtggagatcg ccatggccgg cataccggcg atccgggtta
    22141 gtactgccca gcccagtgcg acagcaaaga tggctcgctc cacctatcag gatctctttg
    22201 atgcatctct gagccagtac gccgcacccg ccttctccgc ctcattcgtc accggcagcg
    22261 cgcccttctg cagcctgatg ctgcccatca cgatggggtt cgacgcctac cgaagcgtgc
    22321 caacgtcggc agcgaccaca acgtcgccca ccgctgcgaa ggccacctcc accgcgctct
    22381 acctgcagga agctggcgat acctcgctga tcagcgtcaa cgacatccat caggggcaga
    22441 tcggcgattg cttcctgctg gcatccattg gcgagatcgc gctttggcac ccctcggcga
    22501 tcatgaacat gatccacgcc aacgccgacg gcaccgagac ggttactctg cacctcgccg
    22561 catctggcca gttgcccacc tacggcacga cacagttcag agccactacc gttaccgtca
    22621 ccaacgtctt ccccagctac gccgtcaaca acggcgccac gcaggatgtg gtcaatggcg
    22681 tgaaggaaat ctgggtccag gtcctggaaa aggctgtcgc aaccctcggt ggcggttaca
    22741 attacatcgc caacggcggc aatccgatga tcgccatgga ggagctgacc ggtcaggata
    22801 ccagatacat ctcctccccg gcatcgctca ccttctcgat gctgcaaggc cacatggcgg
    22861 ccggcgacct gatcgtgatg gacacatact cgacaggcct gctgccgtac aatctggtca
    22921 acaatcacgc ctacatgttc gagagcgtca ccgtcgtcaa cggaacgccc atgatccagc
    22981 tgggcaatcc atggggctac aaccagccgc agctcatacc gctctcccag ctttcccgcg
    23041 gcatcgccga gatcgacatc ggcaatttta ccgacagcaa cctgatcacc ggaggccccg
    23101 gcaatgacac cgtcaccctc agcgcattgg tcaccaatgc atcggtcgat cttggcgctg
    23161 gcaacgacac actcaccctg gcaaatggca ccaattcagc aactgtcgcc aataccgaga
    23221 cagtcattgg cggtaccggc agcgatgcca tcaccctcgc aaccgccgcc aacaatgcca
    23281 gcgtcgatct gggggcgggc aatgacactc tggctttcgg cagcttcacc aactccgcct
    23341 cggtagcccg tgttgaaacc ctgaccggtg gctccggtaa cgacaccatc accctcggca
    23401 gcgcgctcac cgctgcgatg tccgtcgatc tcggtgcggg cagcaataag ctgaccctgg
    23461 cgaacggcgg caacaccggc acagtgaaga atgttgccac actcgttggc ggaacaggca
    23521 gcgacacgct aacgcttggc ggcgccttgg tgaacggatc ggtggatctt ggcagtgg
//
LOCUS       DATGEM010000360         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_364279, whole genome shotgun sequence.
ACCESSION   DATGEM010000360 DATGEM010000000
VERSION     DATGEM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364279"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VKQ27_11460"
     CDS             complement(<1..995)
                     /locus_tag="VKQ27_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY89590.1"
                     /translation="MRSDIRHSVCPHDCPSTCALEVEVLDGRRIGAVHGAKANTYTSG
                     VICNKVSRYAERIHHPDRLTHPLLRTGPKGSGQFRPITWDEALDRTASTFTDVAAKHG
                     TEAVWPYYYAGTMGLVQRDGINRLRHVMRYSRQKLTICTALPELGWQAGVGRSWGPDP
                     REMAKSDLIVVWGGNPVATQVNVMTHIARARKERGAKLVVVDPYRSATAAVADQHIAP
                     RPGTDGALACAVMHIAFRDGFADRAYMAKYADCPDELEAHLASRGPDWASAITGLPVQ
                     QIEAFAHLYCATQRSYIRLGYGFARTRNGVANMHAVSCLPTVTGKWQYEGAGALWS"
     gene            1066..2667
                     /locus_tag="VKQ27_11465"
     CDS             1066..2667
                     /locus_tag="VKQ27_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89591.1"
                     /translation="MLTRRALLATSVAAPAVLSEALGQARAATPKGVAVMAKQIDDIV
                     SFDPAESYEFTNGEVDANCYRRLIRPDLSDATKIQGDLAEKWEVSKDGLTFTFHMRRD
                     AKFDSGNPVTANDAAFSLQRVVKLNKTPGFIITQFGFNADNVEKTIRALDDYTLEMKL
                     PNEAEATSFVLYCLSANVGSVVDTKTAMANQANGDLGNAWLKTHTAGAGVYRLTEWAA
                     NDHVIIDANPHTADKPAMHRVVLRHVADPSTQLLMLRKGDVDIARELGSDQLKSIAGD
                     KTLTVESSPQGTSMYIAMNQSMTELQKVQVHQAIKWAVDYDAIAKNITPGTWSVCQTF
                     LPTALPGSLKATPFKKDIAKAKQLLAEAGLPNGFSVTMDYISTSPSSEIAQAIQADLA
                     AIGIKVSLLPGDQKQVITKTRARQHQLAMLIWGTDYFDPNSNCQAWCENPDDSDQSKL
                     KILAWRSHFADKELTSEAEAAVRELDPEKRIALYHKIQQQFMDRAPFAMLLQRNAVAV
                     LGKGVSGFKVGPMPDYTHYSTIRKV"
     gene            2669..>3486
                     /locus_tag="VKQ27_11470"
     CDS             2669..>3486
                     /locus_tag="VKQ27_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89592.1"
                     /translation="MLTRRTLLATSTASLAAPAIISSADAATPKNTVVMAKQIDDIIG
                     GFDPAQSYEFANNECCGNIYRKLIVPDPKDTDKLVGDLAEKWEISKDGLTFNFAIRKG
                     VLFDSGKELTAEDVAFSLQRVVKLNLTPGFILTQFGWSADNAEKMIKATDAHTLQLVL
                     PAVQASSFVLYCLSATCGGIVEKATAMAHQTNGDLGNVWMRTHTAGTGPYRLVDWAAS
                     DHIILEANPHSALKPKVPRVIIRHVADPSAQLLMVQKGDVDMARDLTPDQLKSVV"
BASE COUNT          695 a         1069 c         1141 g          581 t
ORIGIN      
        1 gaccacagcg cgccagcgcc ctcgtattgc cacttgccgg tgacggtggg caggcagctc
       61 accgcgtgca tgttggcgac gccgttgcgc gtgcgggcaa agccgtagcc cagacggatg
      121 tagctacgct gcgttgcgca atagaggtgc gcgaacgcct cgatctgctg cacgggcagg
      181 ccggtgatcg ccgacgccca atccggacca cgagacgcca gatgcgcctc cagttcgtcc
      241 gggcagtcgg catacttcgc catatacgcg cggtcggcga agccatcgcg gaaggcgatg
      301 tgcatcaccg cgcaggccag cgcgccgtcg gtgcccggac gtggcgcgat gtgctggtcg
      361 gccaccgcgg ctgtggcgct gcgataggga tccaccacca ccagctttgc gccgcgttcc
      421 ttccgggcgc gtgcgatatg cgtcatgacg ttgacctggg tcgcgacggg gttgccgccc
      481 cagacgacga tcaggtcgga cttcgccatc tcgcgcggat cggggcccca ggaacggccg
      541 acgccggcct gccagccgag ttcgggcagg gcagtgcata tcgtcagctt ctggcgcgaa
      601 tagcgcatca cgtggcgcag gcggttgatg ccatcgcgct gtaccaggcc catcgtaccg
      661 gcatagtaat aaggccacac cgcctcggtg ccatgcttcg cagccacgtc ggtgaaagtg
      721 ctggcggtgc ggtccagcgc ctcgtcccag gtgatcgggc ggaactggcc ggatcccttc
      781 ggaccggtgc gcagcagggg gtgggtcagc cgatcgggat gatggatacg ctcggcgtaa
      841 cgagagacct tgttgcagat gacgccggaa gtataggtgt tggccttggc gccgtggacg
      901 gcaccgatac ggcggccgtc cagtacctcc acctcgagcg cgcaggtgga ggggcagtcg
      961 tgcggacaca ccgaatgacg aatatcactt cgcatgggca cggagcctag cgcgcggcct
     1021 cgcggcttgg caaagtgcgg gcaagcacga tttgggagac tgaccatgct gactcggcgc
     1081 gcactgctcg ccacctcggt tgctgccccg gccgtactga gcgaggctct gggccaggcg
     1141 cgggccgcca cccccaaggg cgtcgcggtc atggcaaagc agatcgacga cattgtcagc
     1201 ttcgatccgg ccgagtcgta tgaattcacc aatggcgagg tggacgccaa ctgctaccgc
     1261 cgcctgatcc ggcccgacct gagcgacgcc accaagatcc agggcgacct ggctgaaaag
     1321 tgggaggtca gcaaggacgg cctgaccttc actttccata tgcggcgtga cgcgaagttc
     1381 gactccggca atccggtcac cgcgaacgac gccgcattca gcctgcagcg ggtggtgaag
     1441 ctgaacaaga cgccggggtt catcatcacg cagttcggct tcaatgcgga caatgtcgag
     1501 aagacgatcc gggcgctgga tgactacacg ctggagatga agctgccgaa cgaggcggag
     1561 gcgacgagtt tcgtgctgta ttgcctgtcg gccaatgtcg gcagcgttgt ggatacgaag
     1621 acggcgatgg cgaaccaggc caacggggat ctgggcaatg cctggctgaa gacgcatacc
     1681 gcgggcgccg gggtataccg gctcacggaa tgggcggcga atgatcacgt gatcatcgac
     1741 gccaatccgc acaccgcgga caagcctgcc atgcaccggg tcgtgttgcg gcatgtcgcc
     1801 gatccttcga ctcagctcct gatgctgcgc aagggcgatg tggatatcgc gcgcgagctt
     1861 ggctcggatc aactgaagtc gatcgccggc gacaagacgc tgacggtcga gtcctcgccg
     1921 cagggcacgt cgatgtatat cgcgatgaac cagagcatga ccgaactgca gaaggtgcag
     1981 gttcatcagg cgatcaagtg ggcggtcgat tacgatgcga tcgccaagaa catcacgcca
     2041 ggcacctgga gtgtttgtca gacattcctg cccacggccc tgcccggatc attgaaggca
     2101 acgccgttca agaaggacat cgccaaggca aagcagttat tggcagaggc cggcctgccc
     2161 aatggcttct cggtgacgat ggactatatc tccacctcgc ccagttcgga gattgcccag
     2221 gcaatccagg cggacctcgc cgcaatcggc atcaaggtct cgctgctgcc aggcgatcag
     2281 aagcaggtca tcacgaagac gcgtgcccgg cagcatcaat tggcgatgct gatctggggg
     2341 accgattact tcgacccgaa ttcgaattgc caggcctggt gcgaaaatcc tgatgattcg
     2401 gatcagagca agctgaagat cctcgcctgg cgcagccact tcgcggacaa ggaactgacc
     2461 agcgaagccg aggcggcggt gcgcgaactg gatccggaaa agcgcatcgc gctgtatcac
     2521 aagatccagc agcaattcat ggatcgtgcg ccgtttgcca tgctgctgca gcggaacgcc
     2581 gtcgcggtgc tcggcaaggg cgtgtccggc ttcaaggttg gaccgatgcc cgattacacg
     2641 cactactcca cgatcaggaa ggtatgacat gctgacccga cgcaccctgc tggcgacgtc
     2701 cacagccagt ctcgcggctc ccgcaatcat cagcagcgcc gatgcggcga cgccgaagaa
     2761 caccgtggtg atggcgaagc agatcgacga catcatcggc ggtttcgatc cggcgcagtc
     2821 ctacgaattc gccaacaacg aatgctgcgg caacatctat cgcaagctga tcgttcccga
     2881 cccgaaggac accgacaagc tggtcggcga cctcgcggag aaatgggaga tcagcaagga
     2941 cggccttacc ttcaacttcg caatccgaaa gggcgtgctg ttcgacagtg ggaaagagct
     3001 cacggctgag gacgtcgcat tcagcctgca acgcgtggtg aagctgaacc tcacgccggg
     3061 cttcatcctg acgcagttcg gctggagcgc cgacaacgcg gagaagatga tcaaggccac
     3121 ggacgcccat actttgcagc ttgtgctgcc ggcggtgcag gccagcagct tcgtgctcta
     3181 ctgcctgtcc gcgacctgcg gcggcatcgt cgagaaggcg acggcgatgg cgcaccagac
     3241 caacggcgat ctcggcaatg tgtggatgcg cacgcacacc gccgggacag ggccgtatcg
     3301 gctggtggac tgggcggcgt cggaccacat cattctggag gccaatccgc actcggcgct
     3361 gaagccgaag gtgccgcggg tgatcatccg gcatgtcgcc gatccgtcgg cgcagcttct
     3421 gatggtgcag aagggcgacg tggacatggc tcgcgacctg acgccggatc agctcaagtc
     3481 cgtggt
//
LOCUS       DATGEM010000361         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_690406, whole genome shotgun sequence.
ACCESSION   DATGEM010000361 DATGEM010000000
VERSION     DATGEM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690406"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VKQ27_11475"
     CDS             complement(<1..236)
                     /locus_tag="VKQ27_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89593.1"
                     /translation="MLVTYRIFSNDGLEAAFNAAANALFILPFFLFSALAGQIADCRD
                     KAWIIRIVKTCEIAIMLVGALGLMLQSVPLLLTAL"
     gene            600..1169
                     /gene="pgsA"
                     /locus_tag="VKQ27_11480"
     CDS             600..1169
                     /gene="pgsA"
                     /locus_tag="VKQ27_11480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLY89594.1"
                     /translation="MLSLPNILTLSRIFAVPILAALLWHPGWVGYVLAFLLYCVAGIT
                     DYFDGYLARAQGTVSKLGIFLDPIADKIMVAAVIVMLISTEQVIGGHPVIAGWHVVAA
                     LVILLREIAVSGLREFLAQLSVSVPVSRLAKWKTTFQMVALGALILAGAVPQWPWMHS
                     LGLVCLWLAAGLTLVTGWDYLRVGLKHMD"
     gene            1174..1431
                     /locus_tag="VKQ27_11485"
     CDS             1174..1431
                     /locus_tag="VKQ27_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HLY89595.1"
                     /translation="MTIQLLYFAWVREAIGCDGEAIEPPAGVADVAGLIGWLAARGGG
                     YAGALSDPERLRCAVDQRFAGLGSPIAGVSEIAIFPPVTGG"
     gene            1434..1895
                     /locus_tag="VKQ27_11490"
     CDS             1434..1895
                     /locus_tag="VKQ27_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLY89596.1"
                     /translation="MRVSVQETDFDVAAELTRLEALGGGGVASFTGVVRGGEGLEALR
                     LEAYPGMTQHSLEAIAEEAGRRWPLLGLTLIHRHGVIPVGGRIVLVAVASRHRAAALE
                     ACGFLIDWTKTRAPFWKQELLADGRRQWVEARPEDQAAATAWDASAEHGKP"
     gene            complement(1905..2804)
                     /locus_tag="VKQ27_11495"
     CDS             complement(1905..2804)
                     /locus_tag="VKQ27_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HLY89597.1"
                     /translation="MSIYLPTLKQLQYLVALQEHGHFGNAAEACFVTQSTLSAGLREL
                     ESLLGLTLVERTRRVVRFTPVGERIALQARHVLAEAETLTDLARAAGKPLSGELRMGV
                     IPTIAPFLLPRLLPRLRAEWPDLKLYLREEVTAAVCDSLQRGRLDCVLLALPYRCGDV
                     ESAELFEDQLLFAFPGNQRASLPDPVPPEAIDERNLLLLEDGHCLKDHALAACNRPEL
                     AAEASMLGTSLHTLVQMVDNGLGVTLLPQMAIEAGILNGTDVITRRLDAERPIRRIAL
                     AWRKGSPRAKEFELLAEALRKAV"
     gene            2915..3457
                     /locus_tag="VKQ27_11500"
     CDS             2915..3457
                     /locus_tag="VKQ27_11500"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLY89598.1"
                     /translation="MLTVGDTLPGLTLSVQQGVSALPAGETIDLAQTDGKWKILFYWP
                     KDFTFVCPTEILGYYELNQDFADRDAVLIGASTDTDFVHFAWRKSDERLAAAEFPWIA
                     DNKKELANALGIVDKDAGVAFRATFIIDPDNVIQHVTVNGLNVGRNPQETLRVLDALQ
                     TDELCPCNWEQGAEVLKPAA"
     gene            3575..4093
                     /locus_tag="VKQ27_11505"
     CDS             3575..4093
                     /locus_tag="VKQ27_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY89599.1"
                     /translation="MSLKTFADALPDYAKDLRLNVGSLLNDQTLGDQRKYGLLLSCAH
                     GTGYKPIVEAAEAECAGKLSPEAANAARAAAAVMAMNNVYYRFVHLVSNPEYGTLPAK
                     LRMNVIGNPGIEKADFELFSLAVSAMNGCGMCIDSHEKVLKQHGVSSDAVQSGVRVGA
                     VLKAVATVHATL"
     gene            4194..>4607
                     /locus_tag="VKQ27_11510"
     CDS             4194..>4607
                     /locus_tag="VKQ27_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HLY89600.1"
                     /translation="MPGRLVAYLDSIKARDPAPRSRVEILLYPGVWAVGFHRIAHALF
                     KTRFFFLARLVNHWSRMWTAIDIHPGAVIGRNFFIDHGFSVIGETSVIGDNVTIYQCV
                     TLGGTNPVNGQGGKRHPTLLDGAVVGSGAQVLGPIT"
BASE COUNT          773 a         1465 c         1545 g          824 t
ORIGIN      
        1 agcgcggtca gcagcagcgg caccgactgc agcatcaggc cgagcgcgcc gaccagcatg
       61 atcgcgatct cgcacgtctt cacgatgcgg atgatccacg ccttgtcgcg gcaatccgcg
      121 atctgcccgg cgagcgcgga gaacaggaag aagggcagga tgaacagggc gttggcggcg
      181 gcgttgaacg cggcttcgag cccgtcgttc gagaagatgc gataggtgac cagcatcacc
      241 atcgccgtct tgaacagatt gtcgttgaaa gcccccagaa actgggtggc gaacaggggc
      301 aggaatcgcc tgcgggtcaa gagcttcaga gatgcttgca tatacgaacg cgacgacccg
      361 cctgccccaa ggtgtggcag cacatagcgc agccgggcgt ccacgccaaa tcccatcatg
      421 ggacgggccg gtcccccgat ggggcaaggc gagtgggaat gcccggcaat agcaaagtcc
      481 ggcaagaaaa ggacacgggg ccgaaatcat cgcgcaggcc ggacaaggcg gtttcttgcg
      541 agcgaaaacc gctctaggct ccgcggtgga cgatcttccc gcaggactgc caccggccga
      601 tgctctcgct tcccaacatt ctgacgctgt cgcgaatttt cgcggttccc atcctcgcgg
      661 ccctgctctg gcatccgggc tgggtcggct atgtgctggc cttcctgctc tactgcgtgg
      721 ccggcatcac cgattatttc gacggctatc tcgcccgcgc gcagggcacg gtctcgaagc
      781 tcggcatctt cctcgatccg atcgcggaca agatcatggt cgcagcggtg atcgtgatgc
      841 tgatctccac cgagcaggtg atcggcggcc atccggtgat cgccggctgg catgtcgtgg
      901 cggcgctggt gatcctgctg cgcgagatcg cggtgtccgg gctgcgcgaa ttcctggcgc
      961 agttgtcggt gtcggtgccg gtcagccggc tcgccaagtg gaagacgacc ttccagatgg
     1021 tcgcgctcgg cgcgctgatc ctggcgggcg cggtgccgca atggccgtgg atgcactcgc
     1081 tgggcctcgt ctgcctgtgg ctggctgcgg gccttacgct ggtaacgggc tgggattatc
     1141 tacgggttgg cctcaagcat atggactgaa ccggtgacga tccaactgct ctatttcgcc
     1201 tgggtacgcg aagcgatcgg ctgcgatggc gaggcgatcg agccgcctgc cggcgtggcg
     1261 gacgtggcgg ggctgatcgg ctggctggcg gcgcgaggcg gcggctatgc gggcgcgctg
     1321 tccgatcccg agcggttgcg ttgcgcggtc gaccagcgtt tcgcagggct cggcagcccg
     1381 atcgcgggcg tcagcgagat cgcgatcttc ccgcccgtca ccggaggctg agcatgcgtg
     1441 tttcggtgca ggagacggat ttcgacgttg ccgcggaact cacccggctg gaagcgctgg
     1501 gcggcggcgg ggtggcgagc ttcaccggcg tggtgcgcgg cggggaaggc ctggaagcgc
     1561 tgcgcctcga ggcctatccg ggcatgaccc agcactcgct ggaggcgatc gccgaggagg
     1621 cagggcgacg ctggccgctg cttgggctga cgctgatcca ccgccacggc gtgatcccgg
     1681 tcggcgggcg gatcgtgctg gtcgccgtcg catcgcggca tcgtgccgca gcattggagg
     1741 cctgcggctt cctgatcgac tggaccaaga cccgcgcgcc gttctggaag caggaattgc
     1801 tcgccgacgg ccgccgccag tgggtcgagg cgcggcctga ggatcaggct gccgccaccg
     1861 cctgggatgc cagcgcggag cacggcaagc cgtgatcggg ccgctcagac ggccttgcgc
     1921 agagcctccg ccaacagctc gaattccttc gcgcgcggcg agcccttgcg ccaggcgagc
     1981 gcgatccggc gtatcggccg ctcggcatcg agcctgcggg tgatgacgtc ggtgccgttc
     2041 aggatgccgg cctcgatcgc catctgcggc agcagcgtga cccccaggcc attgtccacc
     2101 atctgcacca gcgtgtgcag cgacgtgccc agcatcgagg cctccgcggc gagctcgggg
     2161 cggttgcagg cggcgagcgc atggtccttg aggcaatgcc cgtcctcgag cagcagcagg
     2221 ttgcgctcgt cgatcgcctc aggcgggacg ggatcgggca ggctcgcgcg ctggtttccg
     2281 gggaaggcga acaagagctg atcctcgaac agttcggccg attcaacatc cccgcagcga
     2341 taaggcagcg ccagcagcac gcagtcgagc cgcccgcgct ggagcgaatc gcacaccgcc
     2401 gccgtcacct cctcgcgcag atagagcttg aggtcgggcc attcggcacg cagtctggga
     2461 agcagccggg gcagcaggaa gggcgcgatg gtggggatca cccccatgcg gagctcgccc
     2521 gacagcggct tgccggccgc gcgcgcgaga tcggtgagag tctcggcctc ggccagcacg
     2581 tgccgcgcct gcagcgcgat gcgctcgccc accggcgtga agcgcacgac gcggcgggtc
     2641 cgctcgacca gcgtcagccc gagcagcgat tccaactcgc gcaaccccgc cgacagtgtc
     2701 gactgcgtga cgaaacacgc ctctgccgcg ttgccgaaat ggccatgttc ttgaagagcc
     2761 acgagatatt gcaactgctt cagggtgggg aggtagatgc tcatagagcg aacttttcga
     2821 tcatagccgg aggaataatc gattggctcc cacattggca atgctgcaga gccgcatcgt
     2881 tagtttcgca cctgcaccct aaaggagacc atccatgctc accgttggcg atacgctgcc
     2941 tggcctcacg ctttccgttc agcagggcgt ttccgctctg ccggccggcg agacgattga
     3001 cctcgcccag accgatggca agtggaagat cctcttctac tggccgaagg atttcacctt
     3061 cgtctgcccc accgagatcc tcggctatta cgagctaaac caggatttcg cggatcgcga
     3121 cgccgtgctg atcggcgcgt cgaccgacac cgacttcgtc cacttcgcgt ggcgcaagtc
     3181 cgacgagcgt ctcgcggccg cggaattccc gtggattgcg gacaacaaga aggagctggc
     3241 gaacgcgctt ggcatcgtcg acaaggatgc cggcgttgcc ttccgcgcca ccttcatcat
     3301 cgatccggac aacgtcatcc agcatgtgac ggtgaacggc ctcaacgttg gtcgtaatcc
     3361 gcaggagacg ctgcgcgttc tggatgcgct gcagactgac gagctttgcc cctgcaactg
     3421 ggagcagggt gccgaggttc tgaagccggc cgcttaaggc ctgctgcctc actcccctta
     3481 gcgcggctgg gcttcccccc tccctgctgg tcgcgctcct tggggcgcgt gggcatcatg
     3541 ctcgcgcgcc cctttttctt agatggagcc tctgatgtcg ctcaaaacct tcgcagatgc
     3601 actgcccgat tatgccaagg atctgcggct gaacgtcggt tcgctgctca atgaccagac
     3661 gctgggtgat cagcgcaaat atggcctgct gctctcctgt gcgcacggca ccggctacaa
     3721 gccgatcgtc gaggcagccg aggccgagtg tgccggcaag ctctcgccgg aagccgccaa
     3781 tgcggcccgt gccgcggctg cggtgatggc gatgaacaac gtctattacc gcttcgttca
     3841 cctcgtctcg aacccggaat atggcacgct tccggccaag ctgcgcatga acgtgatcgg
     3901 caatccgggc atcgagaagg ccgatttcga gctgttcagc ctcgccgttt cggcgatgaa
     3961 cggctgcggc atgtgcattg acagccacga gaaggtgctc aagcagcacg gcgtttcgtc
     4021 ggatgcggtg cagtcgggag tgcgcgtcgg cgcagtgctg aaggcggtcg cgaccgtcca
     4081 cgcgacgctc tgaccggcag gcgctgaaat gggggcggtg gccttgtccg ccgcccctct
     4141 cgcattcccg cgaatgccgt cctaaataag gacccaatca aggggagcct atgatgcccg
     4201 gccgactggt cgcttatctg gattcgatca aggcgcggga tcctgcgcca cgatcacgcg
     4261 tggaaattct gctgtacccg ggcgtctggg cggtgggctt tcatcgtatc gcgcatgcgc
     4321 tgttcaagac gcgtttcttt tttctcgcgc ggttggtcaa tcactggtct cgcatgtgga
     4381 ccgcgattga catccatccc ggcgcggtga tcggccgcaa cttcttcatc gatcatggct
     4441 tcagcgtgat cggcgagacg tcggtgatcg gcgacaatgt cacgatctac cagtgcgtga
     4501 cgctgggtgg caccaatcct gtgaacggcc agggcggcaa acgccatccg acgctgctcg
     4561 acggcgcggt ggtgggttcc ggcgcgcagg tgctggggcc gatcacg
//
LOCUS       DATGEM010000362         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_679669, whole genome shotgun sequence.
ACCESSION   DATGEM010000362 DATGEM010000000
VERSION     DATGEM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679669"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VKQ27_11515"
     CDS             <1..363
                     /locus_tag="VKQ27_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89601.1"
                     /translation="LKTIWPRFFTAYYLADKVSLMSSLYTHRYDSVDHQRLGAMPLVV
                     LSAEYSWGKSGTPEGIWYKRSYSKIWIGLHEALAHLSSRGVHRIIKGSGHTIQLEKPQ
                     AVIDAVDEVLRQLPTGAK"
     gene            773..1999
                     /locus_tag="VKQ27_11520"
     CDS             773..1999
                     /locus_tag="VKQ27_11520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HLY89602.1"
                     /translation="MAGQRTDHNRRELLMGLLAAPAAAILPRVSRAQAARAPTAPPGL
                     EELLNIADFEAAARAVLPPAHFGYLATGIDDDRTVAWNHDAYGQLEISVRRFSDVSRT
                     ELGIDLFGMRWPSPVYLSAVSAQRAFHPEAELATARAAATRSALMMLSAAGSTGPEDV
                     MAARGGPVWQQIYPTDDWEVARAVIERAQRAGCSAIVLTVDAHWPRYNEALVRAMRSD
                     PRTCTNCHVNNSHDFVRKAPIYQGIDVSRVKESTQPSALTWEYLDRVRKLVKGRLLIK
                     GIMRGDEAEDCVRHGADGVIVSNHGGRDEESLRSTIETLPEVVAACRGRVPVLVDGGI
                     RRGTDVFKALALGASAVGVGRPQIWGLAPYGQAGVEAVLDIYMRELRAIMRQAGTPSV
                     ASITRDFVVARAQAPR"
     gene            complement(2052..2696)
                     /locus_tag="VKQ27_11525"
     CDS             complement(2052..2696)
                     /locus_tag="VKQ27_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY89603.1"
                     /translation="MITISAFKWVPEFAQGQVRDLRARWALEEAGLPYRTRLLEQGDQ
                     DKPEYRALQPFGQVPILEEAGLVLFESGAIVLHIGERSEALLPKEAGARARATQWLIS
                     ALNSIEPFLMNVAVIDLFYAQEEWAKLRRPGAVEFAQRRLSGLSKSLGDKPFLDGDRF
                     TAGDLMMTTVLRILKHTDIVSGDRRLAAYIERCTGRPAFKRALDAQIGDFKKAA"
     gene            complement(2729..3178)
                     /locus_tag="VKQ27_11530"
     CDS             complement(2729..3178)
                     /locus_tag="VKQ27_11530"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLY89604.1"
                     /translation="MTSLTLVRRIRARPSIVFEALTTPEGIAQWWGPDAGPVLAAETD
                     VRIGGRFRVRFRMLDGTEHESAGEYLEIVEPERLVMSWQWTNGGEPEEAGEVSRIEID
                     LKRIDAGTEITFTHARLRTQASRASHEQGWAGALDKLVRHFSTEHFA"
     gene            complement(3175..3531)
                     /locus_tag="VKQ27_11535"
     CDS             complement(3175..3531)
                     /locus_tag="VKQ27_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLY89605.1"
                     /translation="MVNYSERTLDRTFAALVDETRRAILARLAREDSATVSELAKPFA
                     IKLPAVMKHLDVLYDAGLISRSKVGRVVTVRIQAGPMREAMDWMRRYERFWSKSLDRL
                     AAYAESKEGAATKKRR"
     gene            3915..4163
                     /locus_tag="VKQ27_11540"
     CDS             3915..4163
                     /locus_tag="VKQ27_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89606.1"
                     /translation="MHATIFDIDGTLLDSYGIDNSMYADAVRAALGPVVIRDAWHKYA
                     RVTDTGVLADICNDNAMAYDGAVSTTVMKVYLGFLRSH"
     gene            complement(4374..>4541)
                     /locus_tag="VKQ27_11545"
     CDS             complement(4374..>4541)
                     /locus_tag="VKQ27_11545"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HLY89607.1"
                     /translation="RLAFTSENLILSFSLPNFYFHAVTAYDILRARGVPLGKRDYEGR
                     LRTRTAQARTL"
BASE COUNT          847 a         1434 c         1395 g          865 t
ORIGIN      
        1 ctcaaaacga tctggcctcg gttcttcacc gcctactatc ttgccgacaa agtctctctc
       61 atgtcgtcgc tgtatacaca ccggtacgac agcgtcgatc atcagcgcct tggcgcaatg
      121 ccgcttgtcg tgctctctgc ggagtactca tgggggaaaa gcggcacgcc cgaaggcatc
      181 tggtacaaac gatcctactc gaagatctgg attggtctgc acgaggcgct cgcgcacctc
      241 tcctcgcgcg gcgtacaccg gatcatcaaa ggatcaggtc acacgattca attggaaaaa
      301 ccgcaggcgg tcatcgacgc cgtcgacgaa gtgctccgcc aactccccac cggtgcgaag
      361 tgaaccgtcc gttggacaag gaacctaacc ggatcccaga cgtcgtacgg cgaacttggc
      421 gagcgcaatg caaccgtctg gcgacggcat cttgacgatg cggtcccgga cggatccgcg
      481 tcgcttgggt ggcgacccga tttacggaag cgacggacag tattccgtgc gactctacgc
      541 agtgcgacac ggcccttgtc agggggtccg gcaaagcggt tcactaaacc tgatgcccca
      601 tgctgcatca aatttgcgat caaatgtctt ggctttcata aatcgtcacg tcctcgtctc
      661 gggaacgtct tactgaaata attcgcacat tgtcgccacg cctcgtgacc atggtatacg
      721 aggttcgagg cagcagggat tcagggtcgg aacacattca ggagaagata aaatggccgg
      781 ccaacgcact gaccacaatc gtcgagaact gctgatgggc cttctggccg cgcccgcggc
      841 cgcgatcttg ccgcgggttt cgcgcgcgca ggcggcccgg gcgcctacgg cgccgcccgg
      901 gctggaggaa ctgctgaaca tcgcagattt cgaggccgca gcgcgagccg tgctgccgcc
      961 cgcgcacttc ggctatcttg ccaccggcat cgatgatgac cggacagtgg cttggaacca
     1021 cgacgcctac ggtcagctgg aaatcagcgt acggcgcttc tccgacgtga gccgcaccga
     1081 gttgggcatc gacctattcg gcatgcgctg gccctcgccg gtatacctct cggccgtctc
     1141 cgcccagcgt gccttccacc ctgaggccga gctcgccacc gcgcgagctg cggcaacgcg
     1201 ctcggcgctg atgatgctgt cggctgccgg ctcgaccgga ccggaggacg tgatggccgc
     1261 gcgcggcgga cccgtgtggc agcagatcta ccccaccgac gattgggagg tcgcgcgcgc
     1321 ggtgatcgag cgcgcgcagc gcgccggctg cagcgccatc gtgctcaccg tcgacgccca
     1381 ctggccgcgc tacaacgagg cgctggttcg tgcgatgcgc agcgatccgc gcacctgcac
     1441 caactgccac gtcaacaact cgcacgactt cgtgcgcaaa gcgcccatct accagggcat
     1501 tgacgtctcg cgcgtgaagg aatcgacgca gccttcggcg cttacgtggg aatatcttga
     1561 ccgcgtgcgc aagctggtca aggggaggct cctgatcaaa ggcatcatgc gcggcgacga
     1621 agccgaggac tgtgtccgcc acggtgccga cggcgtgatc gtctcgaacc acggcgggcg
     1681 agacgaggag tcgcttcgct cgacgattga aaccttgccg gaggtggtcg cggcctgtcg
     1741 cggccgcgtg ccggtgctgg tggatggcgg catccggcgc ggcaccgacg tgttcaaggc
     1801 gctggcgctg ggcgcaagcg cagtcggcgt cggccgccct cagatctggg ggctcgcccc
     1861 ctacggccag gccggcgttg aagcggtact cgatatttac atgcgcgagc tgcgcgccat
     1921 catgcgccag gccggtacgc ccagcgtcgc ctcgatcacg cgcgactttg tggtcgcgcg
     1981 cgcgcaggca ccgcgctgac gcgccggcct attccgggat cgcaggacgc ccggacgtac
     2041 atcgaacctc ttcatgctgc tttcttgaaa tctccaatct gcgcgtcaag ggcgcgctta
     2101 aaagcgggtc ggccggtgca gcgttcgatg tacgctgcca gacgccggtc gccggagacg
     2161 atgtcggtat gcttaaggat gcgcagtacc gtcgtcatca taaggtcccc tgccgtaaag
     2221 cgatcgccat cgagaaaggg cttgtcaccc agcgacttgg agaggcccga caggcgcctc
     2281 tgcgcgaact ccacggcacc ggggcgtcgc agtttcgccc actcctcctg tgcgtaaaag
     2341 agatcgatta ccgcgacatt catcaagaag ggctcaatcg aattgagcgc tgatatcagc
     2401 cattgcgtcg cgcgtgcgcg cgcgccggct tccttcggca gcagcgcctc gctgcgctcg
     2461 cctatatgca gcacgattgc tccggattcg aagagcacga ggccggcttc ttcgagtatc
     2521 ggcacctggc cgaaaggttg cagagcacgg tactccggct tgtcctgatc tccctgctcg
     2581 aggaggcgag tccggtaggg cagccccgct tcctccagtg cccaacgtgc gcgcaagtcg
     2641 cgcacttggc cttgagcaaa ctccggaacc catttgaacg ctgagattgt gatcatcgtg
     2701 catgctcctc gatggaaatt gtttgggtct atgcaaaatg ctctgtggag aaatggcgca
     2761 cgagcttgtc gagcgcgccg gcccagccct gctcgtggct cgcacgtgaa gcctgtgttc
     2821 tgaggcgcgc atgtgtgaag gtgatctcag tccccgcatc tatgcgtttg aggtcaattt
     2881 ctatgcggga cacttcgccg gcctcctcag gctccccgcc attggtccat tgccagctca
     2941 tgaccaggcg ttcgggctcg acgatctcga ggtactcgcc ggcgctctca tgttcggtgc
     3001 cgtcgagcat gcggaagcgc acccggaacc ggccgccgat gcggacgtcc gtctcggcag
     3061 caagcaccgg accggcgtcc ggtccccacc actgagcgat gccttctggt gtggtcagcg
     3121 cctcgaagac gatcgacggc cgggcgcgaa tgcggcgcac gagggtaagg ctggtcatcg
     3181 tcgtttcttc gtcgccgccc cttccttgct ttcggcatag gcggcaagac gatcgaggct
     3241 cttggaccag aatcgttcgt agcggcgcat ccaatccatc gcctccctca taggcccagc
     3301 ttggattcgc accgtaacga cgcgtccaac ctttgaccgc gagatgagac cggcatcgta
     3361 cagaacgtcc aggtgcttca tcacggcggg caacttgatc gcgaacggct tcgcgagctc
     3421 gctcaccgtc gcgctgtctt cgcgcgcgag ccgagccaag attgcccggc gagtctcatc
     3481 caccagcgcc gcgaaagttc ggtcgagtgt tcgttcagaa tagttcacca tctagtgtac
     3541 tatgccgact ggtgcctccg gtgtcaatgt gagtctcggc gtggatgcgc taactcaacc
     3601 gcgcgcgggg gcggcctact acggtggacc aacatcgaag caggtgaata gattctgcgc
     3661 gctgtcccag cggcgctagc cggccaacta cacacagtct cttcccttac tgaagcttcc
     3721 cgaaccggac attcaccatc ccgcgcacgc cacgctagcg gctccggctc cgtgtcagtc
     3781 agcgtgaggc ctgttgacgg cgtgctgcga aatggtgatg aattggccaa gaattccggt
     3841 cgtctgcggc tgcctctttc gtggtgtaaa atcgactcca ggctgcaaac gatcaaggaa
     3901 gtgattaggc acacatgcac gcgaccatct tcgatatcga cgggacgcta ctcgattcct
     3961 acggtatcga caattcgatg tacgctgacg cggttcgcgc ggcgctcggc ccagtggtta
     4021 ttcgagatgc gtggcataaa tacgcccgcg tgactgatac cggcgttctc gctgacatat
     4081 gcaacgacaa cgcgatggcc tatgacggcg cggtatcgac aacggtcatg aaagtctact
     4141 tggggttcct ccgatctcat taaaactacg cgacgacaag ggcgccacac atcttttggc
     4201 ggtgcgacat cagcgtgtcg ccgccgcgga agaaagccgc tattcgcgat aggccgcttc
     4261 gcgtcgatca cggtcgatta gcgccgatct tcaagtaggg tgtcgcgtag ccggttcggg
     4321 aaatgggcac caggcggtag tcggccggga gctgtcgttt cggcgaaagc gtcctatagg
     4381 gtacgagcct gggcagtccg agtgcgcagc cggccctcgt aatcgcgctt tccgagcggc
     4441 acaccccgcg cgcgcagaat gtcgtatgcg gtgacggcgt ggaaatagaa gttgggcagc
     4501 gagaacgaaa ggatgaggtt ctccgacgtg aaggctagcc t
//
LOCUS       DATGEM010000363         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1106541, whole genome shotgun sequence.
ACCESSION   DATGEM010000363 DATGEM010000000
VERSION     DATGEM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106541"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VKQ27_11550"
     CDS             complement(<1..929)
                     /locus_tag="VKQ27_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89608.1"
                     /translation="MIGFAGMTHLGLVSAICAAEKGFTVIGFDLDSARTSALTRGELP
                     VSEPRLPELLQKNRERLLFTSDAAVLSSCDVVYVAPDVPTDDQGESDLGSINDMLEAV
                     FARTRDNAVIVVLSQVPPGFTRGKLVAGKILYYQVETLIFGRAVERALQPERYIVGCA
                     DPTEPLPAVFASFLGAHECPILPMRFESAELAKISINMCLVASVSTANTLAELCEKIG
                     ADWSEIVPALKLDKRIGQYAYLAPGLGIAGGNLERDLATVCSLSDNHGTDTGVVRAWI
                     ANSRYRRDWVLRTLHATVLSRREDPVIAVLGLS"
     gene            complement(938..1729)
                     /locus_tag="VKQ27_11555"
     CDS             complement(938..1729)
                     /locus_tag="VKQ27_11555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY89609.1"
                     /translation="MLLHAKDRLTGGADALTPPRALHFVGGGDFRAIGRGYLQHFQDV
                     GGLRPDEHVLDIGCGTGRMAIPLMEYLGERGTYAGFDIYGPAIDWCTRNISSRDTRFK
                     FFHADIYNLEYNARGKMRADQFRFPLDDESIDFAFATSVFTHMHPEEIRRYLDEMRRV
                     LRYEGRALATFFICDGPQPAEAGARSVMDFRYPISEGYTVDPDTPERAIAYREDWIRR
                     NVGEAGLRVQDPVRYGSWSGRRDGFDFQDIVLIRKEPQPRAGEKA"
     gene            complement(1804..2655)
                     /locus_tag="VKQ27_11560"
     CDS             complement(1804..2655)
                     /locus_tag="VKQ27_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89610.1"
                     /translation="MVVLGAGGFIAGAIAKRLKAEGIATLGLGRPGLDLLQPGAAQAL
                     ADALSADDVLVFASARAPCKDLAMLRENLAMAESVCAALHRRPVAHAIYLSSDAVYKD
                     SRGPLTEASCAEPGSLHGVMHLAREIALRQHHVGALALVRPTLVYGIDDPHNGYGPNR
                     FRRLAASGQDIVLFGEGEERRDHVDVEDVAELVRLIVLHRSTGIANAVSGQTVSFREL
                     AEFAASEFAPHVAIRNAQRTGPMPHGGYRPFDNAAVLKALPGFRFKGWREGLSKVHAA
                     AKAKADA"
     gene            complement(2697..>3184)
                     /locus_tag="VKQ27_11565"
     CDS             complement(2697..>3184)
                     /locus_tag="VKQ27_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89611.1"
                     /translation="DQGAGVLPDLGSHLLDTALFWFGRPSEPFRIHSANRFENRAFDH
                     VAFGSNGAPVLQMEITLLSWRNHFYADIFAEKGSAHIQSLCKWGPSTITVRDRKLPSG
                     RPDEESVTLVESDPTWEIEYRHFRQLCENGGGNLDNDIWINDVLRRLSSDLGAKAPGA
                     R"
BASE COUNT          586 a         1009 c         1036 g          553 t
ORIGIN      
        1 tatgagagtc ccagtaccgc gatgaccggg tcctcccgcc tcgagagcac ggtggcatgc
       61 agcgtacgca acacccagtc gcggcggtac cggctgttgg caatccaggc gcgcaccacg
      121 ccggtgtcgg tgccgtggtt gtccgaaaga ctgcacaccg tggcgaggtc gcgctcgagg
      181 ttgccgccgg cgatgccgag gcccggggcg aggtaagcgt actgacctat gcgcttgtcg
      241 agtttgaggg cggggacgat ctccgaccag tcggcaccaa ttttctcgca aagttcggcg
      301 agcgtatttg cagtgctaac cgaggcaacc aggcacatat tgatcgagat cttggcgagt
      361 tcggcgcttt cgaagcgcat cggcaggatg gggcattcgt gcgcgccaag aaaggacgcg
      421 aataccgcgg ggagcggctc tgtcggatcg gcgcagccaa cgatgtagcg ctcgggctgc
      481 agcgcacgct cgacggcacg cccgaatatc agggtctcga cctgatagta aaggatcttc
      541 cccgcgacca atttgccgcg ggtgaaacct ggcggcactt gcgatagaac gacgatcacg
      601 gcgttgtcgc gcgtcctggc gaacacggct tcgagcatgt cgttgatcga accgagatcg
      661 ctttccccct gatcgtcggt cggcacatcc ggggcaacgt agaccacgtc acagctggag
      721 agcacggcgg cgtccgatgt gaacagcaac cgttccctgt ttttctgcag caactccggg
      781 agacggggtt cggacaccgg aagctcgccg cgcgtgaggg ctgaggtacg cgcggaatcg
      841 aggtcgaagc cgatcaccgt gaagcctttc tccgcagcgc agatcgccga gaccagcccc
      901 aggtgcgtca tgccggcgaa gccaatcacg gggccgctca cgccttctct cccgcgcgcg
      961 gctggggctc tttgcggatc agcacgatgt cctggaaatc gaaaccgtct ctgcgcccgg
     1021 accagcttcc gtaacgaacc ggatcctgca ccctcaagcc cgcctcgccg acgtttcgcc
     1081 tgatccagtc ttcccgatag gcgatcgccc gttcgggagt atcaggatcg acggtgtatc
     1141 cctcggagat cggatagcga aaatccatca cggaccgtgc cccggcttct gctggttgtg
     1201 gtccgtcgca gatgaagaat gtcgccaagg cccggccttc gtagcgaagc acccggcgca
     1261 tttcgtcgag gtaccggcgt atctcttctg gatgcatgtg ggtgaaaacc gaggtcgcga
     1321 aggcaaagtc gatcgattcg tcgtcaaggg ggaaacggaa ttgatccgct ctcatcttcc
     1381 cgcgagcgtt gtattcgaga ttgtagatgt ccgcgtggaa gaacttaaac cgcgtgtcgc
     1441 gcgaagagat gttacgcgtg caccagtcta tcgccgggcc gtagatatcg aatccagcgt
     1501 aggttccgcg ttcgccgaga tattccatga gcgggatcgc catgcgaccc gtaccgcaac
     1561 caatatcgag gacatgctcg tcgggacgta agccgcctac atcctgaaag tgctggagat
     1621 atccccggcc gatcgcgcgg aaatccccgc cccccacgaa atgcagcgcg cgcggcgggg
     1681 tcaacgcgtc cgctcccccg gtcaggcggt ccttggcgtg gagcaacatg agataggcgt
     1741 cccgaaccca ccgcacaaaa cgccgcggaa accgaaacaa ggaggatccg ggcttcggag
     1801 gactcacgcg tctgccttcg ctttcgcggc ggcgtggacc ttcgataagc cttctcgcca
     1861 tcccttgaag cggaacccgg ggagcgcctt gagcaccgca gcgttgtcga atggccggta
     1921 gccaccgtgc ggcatcggcc cggtgcgctg tgcattccgg atcgcgacgt gcggagcgaa
     1981 ttcggaggcg gcgaattccg ccagctcgcg aaacgagacg gtctgccccg agacggcgtt
     2041 ggcgatgccc gtgctccgat gtagcacgat caggcgcacc agttcggcga catcttccac
     2101 gtcgacgtgg tcgcgtcgct cctcgccttc gccgaagagc acgatatcct gtcccgacgc
     2161 cgcaaggcgc cggaatcggt tcgggccgta gccgttgtgc gggtcgtcga tcccgtatac
     2221 gagcgtcggc cgcaccaacg cgagcgcgcc gacatggtgc tggcgcagcg cgatctcgcg
     2281 ggcgaggtgc atcacgccgt gcagcgatcc gggctcggcg cacgaggcct cggtgagcgg
     2341 cccgcgggaa tccttgtaca cggcgtccga gctgagatag atcgcgtgcg cgactgggcg
     2401 cctgtgcaag gcggcgcaca cggattccgc catcgcgagg ttctcgcgca gcatcgcgag
     2461 gtccttgcaa ggtgcccgcg ccgaagcgaa aacgagcaca tcgtcggccg acaaggcgtc
     2521 tgctagcgcc tgcgcggcgc ccggttgcag cagatcgagc cccggcctgc ccaatcccag
     2581 cgtcgcgatg ccctcggcct tcaggcgctt cgcaatcgcg cctgcgatga agcccccggc
     2641 gccgagcacg accacgcgcg gcggcttctt cggagtcgaa tgaagatgcg tcagcatcag
     2701 cgcgcgcctg gtgctttggc gccgaggtcc gaggacaggc ggcgcagcac gtcattgatc
     2761 cagatgtcgt tgtcgagatt gccgccgccg ttttcgcaga gctgtcggaa gtggcggtac
     2821 tcgatttccc aggttggatc ggactcgacc agcgtcaccg attcctcgtc agggcgacca
     2881 ctcggcagct tgcggtcgcg cacggtaatc gtgctgggcc cccacttgca cagcgactga
     2941 atgtgcgcgc tgcccttctc tgcaaagatg tcggcgtaga agtgattgcg ccaggagagc
     3001 agggtaatct ccatctgcag caccggcgcg ccgttcgagc cgaaggcgac atgatcgaag
     3061 gcgcggttct cgaaccggtt ggccgaatgg atgcgaaacg gctccgaggg gcggccgaac
     3121 cagaagagtg cggtgtcgag caggtgcgat cccaggtcag gcagcacgcc cgcgccctgg
     3181 tcgc
//
LOCUS       DATGEM010000364         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_120102, whole genome shotgun sequence.
ACCESSION   DATGEM010000364 DATGEM010000000
VERSION     DATGEM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120102"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VKQ27_11570"
     CDS             <1..787
                     /locus_tag="VKQ27_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89612.1"
                     /translation="FSVLALVMTRNWLAWGVYPLVLTAAAWFLAPLEQPAISIVLALA
                     IVTPMGPLIWRLAFRPVADASVLSLLIVSMALHFVIIGMGLVFFGPEEASAPTITDAS
                     FDLGQLNISGQSLCVLGSCIVLVAGLRWFFNATMHGKALRAAASDRLGARLVGIDANA
                     TGALAFGLASFIAALAGVLIGAVTPLSYDAGFDIGLKGFVAAIMGGLVSYPLAAAGAV
                     LVGVLESLGSFWASAYREVIVFTLLIPVLVWLSLRAGSGEERA"
     gene            784..2547
                     /locus_tag="VKQ27_11575"
     CDS             784..2547
                     /locus_tag="VKQ27_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLY89613.1"
                     /translation="MKPPAWLALLGIAVLAPLLLPTFYVTLAGYIGLATIVALGLVLL
                     TGISGQTSFGQASFVGLAAYATTMLTKFAGQPPIIGLIAGLAITALVAWLLGLITARL
                     SGHFLALVSVAWGISFFSLFGTLPWLHGFNGIGDIPPLTLGPLSASDQRINLAVILLV
                     VAAIMAMSANLLDSRLGRAIRALNGARLMGESMGIDTVLLSRHVFVVAGIYAGLAGFL
                     FAHFQRFVSPTPFNLGASIDYLFMVIIGGSESLWGAPIGATLVVVLRDQFNDWIPRIT
                     GRAGDFEALVFSLVVILLLQRAPEGLLPILMRLKRQPPPPFVPPEPAPDLSIAATAPM
                     PGEELLSVESVSRYFGGLAANRDVTFSVRASEIVALIGPNGAGKTTLFNVVSGVIPPS
                     SGTVRLFGSIVQHRRPHHVAASGVARTFQHVRLLGRRSVLENIALGAHLSGRIGMLRA
                     MLRLDRAEERALLDHARRQAARLGLTPMLEMRAGDLPLGQQRVVEIARALCLRPRLLL
                     LDEPAAGLRLAEKQRLAILLRQLRAEGMGVLLVEHDMDFVMGLADRVVVMDFGQKIAE
                     GQPEQVQSDPVVMEAYLGAAA"
     gene            2544..>2994
                     /locus_tag="VKQ27_11580"
     CDS             2544..>2994
                     /locus_tag="VKQ27_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89614.1"
                     /translation="MTAPLLHIDGLRAGYGKVEVLHGVSLSVPEGRMVAIVGPNGAGK
                     TTLMGAVMGLLPRSGGDVRYDGSVAGTTEALVASGAVLVPERRALFADMSVEDNLQLG
                     FYVRRRAGERDIQPALREVFAIFPRLQERRRQVAGTLSGGERQMLALG"
BASE COUNT          413 a          980 c          981 g          620 t
ORIGIN      
        1 attttccgtg ctggcgctgg tcatgacgcg caactggctg gcctggggcg tatatccgct
       61 ggtattgacc gcagccgcct ggttcctggc gccgctggaa cagcctgcga tctcgatcgt
      121 gctcgcgctg gcaatcgtca ccccgatggg cccgctgatc tggcgcctgg cattccgtcc
      181 tgtcgcggat gcatcggtgc tgtcgctact gatcgtgtcg atggcgctgc acttcgtgat
      241 catcggcatg ggactggtgt tctttggccc ggaagaggcc agcgctccaa ccatcaccga
      301 tgccagcttc gatctgggac aactcaacat ctctggccag tcgttgtgcg tgctgggcag
      361 ctgcatcgtg ctggtcgccg gcctgcgctg gttcttcaac gcgacgatgc acggcaaagc
      421 cttgcgcgcc gctgcctccg acaggctggg cgcgcggctc gtcggcatcg acgcgaacgc
      481 caccggtgcg ctggcctttg gactcgcatc cttcatcgcc gcactggccg gcgtgctgat
      541 cggtgcggtg acaccgcttt cctacgacgc aggcttcgat atcggcctga agggtttcgt
      601 cgccgcgatc atgggaggcc tggtgtcgta tccgctggcc gctgcgggcg cggtgctggt
      661 gggcgtgctg gaatcgcttg gcagcttctg ggccagcgcc taccgcgagg tgatcgtctt
      721 caccctgctg atccccgttc tggtgtggct gtcattgcgg gctggttctg gcgaggagcg
      781 cgcgtgaagc cacccgcctg gctggccttg cttggcatcg ccgtgctcgc accgctgctc
      841 ctgccaacgt tctacgtgac gctggccggc tatatcggcc tggccaccat cgtcgcgttg
      901 ggcctggtcc tgctcaccgg catcagtggt cagacgtcat tcggtcaggc gagcttcgtc
      961 ggcctcgcag cttacgcgac gaccatgctg acgaagttcg ccggccagcc accgatcatt
     1021 ggcctgatcg cggggctggc gatcaccgcg ctggtcgcct ggctgctcgg cctgatcacg
     1081 gcgcgcctgt ctggccactt cctcgcattg gtgtcggtcg cgtggggcat ctcgttcttc
     1141 tcgctgttcg gcaccttgcc atggctgcac ggcttcaacg gaatcggcga catcccgccg
     1201 ctgacgctcg gcccactgtc ggcctcggac cagcgcatca acctcgctgt catcctgctc
     1261 gtcgtcgcgg ccatcatggc catgtcggcg aacctgctcg acagcaggct gggccgtgcg
     1321 atccgtgccc tgaacggcgc acgccttatg ggcgagagca tgggcatcga caccgtgctg
     1381 ctcagccgcc acgtgttcgt cgtcgccgga atctatgccg gccttgccgg cttcctgttc
     1441 gcgcatttcc agcgcttcgt cagccccacg ccgttcaacc tcggcgccag catcgactac
     1501 ctgttcatgg tgatcatcgg tggctcggaa tcactgtggg gcgcgccgat cggcgccacg
     1561 ctggtggtcg tgctgcgtga tcagttcaac gactggatcc cgcgcatcac cggccgcgcc
     1621 ggcgatttcg aggcactggt gttcagcctc gtcgtcatcc tgctgctgca acgagcgccg
     1681 gagggattgc tgccgatcct tatgcgcctg aagcggcaac cgccgccacc attcgtgcca
     1741 cctgaacccg caccggacct gtcgattgcc gccaccgctc cgatgcctgg cgaggaactt
     1801 ctgtcggtcg aatcagtctc ccgctacttc ggcggtctgg cagccaatcg cgacgtgacc
     1861 ttcagcgtgc gcgccagcga gatcgttgca ctgatcgggc cgaatggcgc gggcaagaca
     1921 acactgttca acgtcgtcag cggcgtgatc ccgccaagct ctggtacggt gcggctgttc
     1981 gggtctatcg tgcagcatcg ccggccgcac catgtcgctg catctggtgt cgcacgcacc
     2041 tttcagcatg tccgtctgct cggccgccgc agcgtgctgg aaaacatcgc gctcggtgcg
     2101 cacctctccg gccgtatcgg catgctgcgc gccatgctgc gtctggatcg tgcggaggaa
     2161 cgcgcgttgc tcgaccatgc gcgccggcag gcggcacgcc tcgggttgac cccgatgctg
     2221 gagatgcgag cgggtgatct gcctctcggt cagcagcgcg ttgtggaaat cgcacgcgcg
     2281 ctctgtctgc gaccgcgtct cttgttgctg gatgaacctg ctgccggatt gcgccttgcc
     2341 gagaagcaac ggctcgcgat cctgttgcgg caattgcgcg cggaaggcat gggcgtgctg
     2401 ctggtcgagc acgatatgga tttcgtcatg ggcctggccg accgcgtcgt ggtgatggat
     2461 ttcggccaga agatcgccga agggcagccc gaacaggtgc agtccgatcc cgttgtgatg
     2521 gaagcctatc tcggcgccgc ggcatgaccg cgccgctgct gcacatcgat ggcctgcgcg
     2581 ccggctacgg caaggtggag gtgctgcacg gcgtcagcct ctcagtgccc gagggccgca
     2641 tggtggcgat cgtcggcccc aacggtgccg gcaaaacaac gctgatgggc gcggtaatgg
     2701 gactgctgcc gcgcagtggc ggcgatgtgc gctacgacgg tagtgtcgcg ggaacgactg
     2761 aagcgctggt tgcttctggt gccgtgctgg ttcccgaacg acgtgcgctg ttcgcggata
     2821 tgagcgtgga agataacctg cagcttggct tctacgtccg tcgccgcgct ggcgagcgag
     2881 acatccagcc agccttgcgc gaggtgttcg cgatctttcc gcggttgcag gaacgtcgcc
     2941 ggcaagtcgc tggtacgctc tccggcggtg aacgccagat gttggcattg ggcc
//
LOCUS       DATGEM010000365         6531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1494488, whole genome shotgun sequence.
ACCESSION   DATGEM010000365 DATGEM010000000
VERSION     DATGEM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494488"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VKQ27_11585"
     CDS             complement(<1..383)
                     /locus_tag="VKQ27_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HLY89615.1"
                     /translation="MMVFRRLLRDRSALLGLIILVTLIVVAIFAYPLATFPQDVTSYD
                     PPHRLLPPSMTYWFGTDRMGGDIYSRLLFGARLTILIAVVATSVSVLVGVPVGLLAGY
                     YNNRLSDLLMRISDIFLAVPQIVLA"
     gene            complement(442..1443)
                     /locus_tag="VKQ27_11590"
     CDS             complement(442..1443)
                     /locus_tag="VKQ27_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89616.1"
                     /translation="MRLLAMVVRRIAWMPPTLLGLVLLVFAISHVVPADPARIMAGEN
                     ATPEQVAKVRHDFGLDLPLPVQFGHYVRDVVVGDMGTSLFTQRPVADDLLERLPATFE
                     LAIYAMVLAILIGVPLGVVAALHRNSLLDHVVRIVTIGGLAMAAFWLAILLQLLFSMW
                     LGVTPVQGRIDGWGPDPITGFYTLDALLRGDWDTLGQALRYLALPVTTLALPAMATIV
                     RFTRAGVLNVMSSNFVFYQAAMGIPRRRVVWKYILRSALIGTLTQIGLIFGTLIANAV
                     VVETVFDWPGLGSYAVGSILQSDYNAIMSFTLFIGVVFIMTNLLVDVAQAVLDPRGR"
     gene            complement(1489..2484)
                     /locus_tag="VKQ27_11595"
     CDS             complement(1489..2484)
                     /locus_tag="VKQ27_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY89617.1"
                     /translation="MERRTFGRTGLQLSILGFGCGAVGGLMVRGTPSDQERAVAKAIE
                     AGINYFDTAAQYGNGESEKNLGRILAKLKPNVIVGTKVRILSADFNRIAAAVGESLDA
                     SLKRMGKEQVDIFHLHNAITTGGGGESLSAAQVLNDVVPAFEKLREQGKLRFPGITAV
                     GDTAALHQVIDARAFFSAQVSYNMLNPSAAESLPPRYPAQDYGKMFDHTRAAGVGVVG
                     IRVLAGGALSGVTERHPIASPPPDPIGSANSYNADLERAGRLMPLVHEGYAATLSEAA
                     IRFAMTHEAMGSILVGMATIDQFEQSLAAVNKGPLPSAALVRLAELQKGFVGEQR"
     gene            complement(2510..3484)
                     /locus_tag="VKQ27_11600"
     CDS             complement(2510..3484)
                     /locus_tag="VKQ27_11600"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLY89618.1"
                     /translation="MRICVYGAGAIGGHLAVRLARGGAEVSVLARGPHLAAIQRDGLT
                     VHASDGQHNVRVQASDDAKALGPQDAVIVTVKAPALPAVAAGIGPLLNSDTSVAFVMN
                     GIPWFYFHGIGGKLEGHRLPKIDPDDALWRTVGPERAMAGVVYAASAVIAPGVIEVEN
                     AEPRVVLGEPDDRESQRVQAIASHINAGGMTAIVSTKIRDEIWNKLLGNLGNGPLAVL
                     TQVSIKNLYQEPTCVDAARRVMEEAQAVANALGAHPSSNHAKRIEHGRNLDHKPSILQ
                     DLELGRPMEIDGIFDAPLELARAAGVATPTLDLLVAMAKVRARAAGLY"
     gene            complement(3578..3955)
                     /locus_tag="VKQ27_11605"
     CDS             complement(3578..3955)
                     /locus_tag="VKQ27_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HLY89619.1"
                     /translation="MKGLLPAVLVLLAAAPAVHWSHAQPMTVVMVDDRFQPDHLTFER
                     GTQYLLRLENHGKDLHEFTAPEFFAAAVMRDPGQLANGGQEVVVQPGGSADIYLEPLK
                     AGTYHLTCADHDWDGMVGEIVVR"
     gene            complement(3952..4890)
                     /locus_tag="VKQ27_11610"
     CDS             complement(3952..4890)
                     /locus_tag="VKQ27_11610"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89620.1"
                     /translation="MSIFVIGGTGFIGTRVIRLLAARGEEIVCMDINPGAASFDDLGK
                     QVRVMRGDVTQFDDVMAATSSAKPSRIINLSYFLGSDHAPHIATKLNIVGMDNCFEAA
                     RLAGVNRVVFASSLAVNGMQKHYGDRMITEDDFRHGDVQYAMHKIFNEFQAQDYNEKF
                     GMTITAVRPANVTGPDKVRGSVDHVNIITQPAQGKPISFPYKDAMRCPIHVDDIAEVF
                     VRVLLADKPKHGVYNSGGVAISLGEIADLVREFLPDARVTFEHEVGGKATSGNYLIDN
                     TRLVQEFGVQYLPYRQRVLQIINDVRRQVGLPTVAA"
     gene            4932..5315
                     /locus_tag="VKQ27_11615"
     CDS             4932..5315
                     /locus_tag="VKQ27_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY89621.1"
                     /translation="MRPQAEAKIVRLAEAETVSFGPLADYARLTGDDGLPFFTGVQTC
                     KPGYQTPLHWHPYVECLFILEGTMEAWLEGQDDKPSRLGPGDMIALPACTPHVFRNAG
                     EGTLRLLGIHGSPTRIVHRLEEQSP"
     gene            complement(5312..6004)
                     /locus_tag="VKQ27_11620"
     CDS             complement(5312..6004)
                     /locus_tag="VKQ27_11620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLY89622.1"
                     /translation="MNWFEVPDGVMQRVIARTGTPHPHAELDPKRTALVVVDLQNAFM
                     DDEIGHAVCPMARDIVPAVNRLASVVRGVGGGVFWIKNTFDERSALEWSHAQEMLTPA
                     ARGRRIEAMSEGSKGQELWPELDVRPEDEIVRKYRFSAFMPGTCELPYRLRARGFDTV
                     LITGTVTNVCCESSARDAMMTNFKTIMVSDANAAMNMEEHTASLVAFYAIFGDVMDTD
                     FVVQRLTRNDQV"
     gene            complement(6001..6300)
                     /locus_tag="VKQ27_11625"
     CDS             complement(6001..6300)
                     /locus_tag="VKQ27_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89623.1"
                     /translation="MKIQDIDRVNHLIAELNGMKELIAHAERAELADFEVFIKLSGDA
                     SIRMSSEGAASTHYSGFSASPEFLGRLQRLSIDELDARRRAIIAELAKLGVEADG"
     gene            complement(6297..>6531)
                     /locus_tag="VKQ27_11630"
     CDS             complement(6297..>6531)
                     /locus_tag="VKQ27_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89624.1"
                     /translation="HCIELPFVFGTFDAWADAPMLAGGDRKQMADLSEAMRQAWIAFV
                     RTGEPASSWPRHDVDQRAVMRFGTRIGAVGDIA"
BASE COUNT         1336 a         2207 c         1833 g         1155 t
ORIGIN      
        1 atggccagca cgatctgcgg caccgccagg aagatgtcgg agatccgcat cagcaggtcg
       61 ctgagccggt tgttataata cccagcaagc agcccgaccg gtacgccaac gagtacggag
      121 accgaagtgg cgacgacggc gatcaggatg gtcagccgtg caccgaacag cagccggcta
      181 tatatgtccc cgcccatacg gtcggtaccg aaccagtagg tcatactcgg cggaagcagc
      241 cggtgcggtg ggtcatagct ggtgacgtcc tgcggaaatg tggccagcgg ataggcaaat
      301 atcgccacca cgatcagcgt gacaaggatg atgaggccaa gcagggccga tcggtcccgt
      361 agcagccggc ggaagaccat catggatcgc tccgggcaaa cgcccgctcc gaacacgcca
      421 tgcccgaagt tgatccaacc atcatcgccc gcgtggatcc agcaccgcct gcgccacgtc
      481 caccagcagg tttgtcatga tgaacaccac gccgatgaac agcgtgaagc tcatgatggc
      541 attgtagtcg gactgcagga tcgatccgac cgcataactg ccaagcccgg gccaatcgaa
      601 cacggtttcc acgaccacag cattagcaat tagcgtgccg aatatcaggc caatctgagt
      661 caacgtgccg atcaatgcgc tgcgaagaat gtatttccag acgacacgac gccgtggaat
      721 acccatcgcc gcttggtaga acacgaagtt gctcgacatc acgttcagca cgccggcacg
      781 cgtgaaacgc acgatcgttg ccatggccgg cagggccaac gtagtcacgg gcagcgcgag
      841 ataccgcaac gcctggccca acgtgtccca atcgccacga agcaaggcgt ccaatgtgta
      901 gaacccggtg atcggatcgg gtccccagcc atcgatgcgc ccctgcacgg gcgtaacgcc
      961 aagccacatc gaaaacaaca gttgcagcag tatcgccagc cagaacgcgg ccatcgccag
     1021 cccaccgatg gtgacgatac gcaccacatg gtcaagcaac gaattccgat gcagggccgc
     1081 cacgacccca agcggcacac cgatcaatat cgccagcacc atcgcataga tcgcgagttc
     1141 gaacgtcgcc ggcagtcgct ccagaagatc gtccgcgaca ggacgctgag tgaacaggct
     1201 cgtccccatg tcgcccacga ctacatcgcg cacgtaatgc ccgaattgca ctggcagcgg
     1261 cagatcgaga ccaaagtcgt ggcggacctt ggccacttgc tccggcgtcg cattctcgcc
     1321 cgccatgatg cgcgccggat cggcaggcac cacgtgcgag attgcgaata cgagcagcac
     1381 aagcccgagc agcgtcggcg gcatccaggc aatccgccgc acaaccatag ccaacagtcg
     1441 cattactcag gcaaccgacg catcgcggat gatgccacag cccatcaatc agcgttgctc
     1501 gccaacgaac cccttttgca gctcagccag gcgcaccagc gcggccgatg gcagcggccc
     1561 cttgttgacc gccgcaagcg actgttcgaa ctgatcaatc gtcgccatgc cgacaaggat
     1621 gcttcccatt gcctcatgag tcatggcgaa gcggatcgca gcctcggaca aggttgcagc
     1681 ataaccctcg tgcaccagtg gcatcaggcg gcccgcccgt tccaagtcgg cattgtatga
     1741 attcgccgac ccgatgggat caggcggcgg actggcaatg ggatgccgct ccgtcacacc
     1801 gctgagcgca cctcctgcca gcacgcggat gccgaccaca ccgaccccgg cagcgcgcgt
     1861 atgatcgaac atttttccat aatcctgcgc cggataacgc ggcggcaacg attccgcggc
     1921 gctggggttg agcatgttgt agctcacctg cgcggagaag aaggcgcgcg cgtcgatcac
     1981 ctgatgcagc gctgctgtat caccaaccgc tgtgatgcca gggaagcgaa gcttgccctg
     2041 ttcacgcagc ttttcgaatg ccgggacaac atcgttcagg acctgtgccg ccgacagcga
     2101 ttcaccgcca ccgcctgtgg tgatcgcgtt gtgcagatgg aatatgtcca cctgctcctt
     2161 gcccatgcgc ttcaggctgg catccagcga ctccccgacc gccgcggcga tacgattgaa
     2221 gtcagctgac aaaatacgga cctttgtgcc gacgatcacg ttgggcttca gcttcgcgag
     2281 gatacggcca aggttcttct cggactcgcc gttgccgtac tgcgcggccg tgtcgaagta
     2341 attgatgcca gcttcaatcg ccttcgcgac tgcacgctcc tggtcggatg gcgtgccgcg
     2401 taccatcagg ccgccgacgg caccgcagcc aaaaccgaga atggaaagct gcagtcccgt
     2461 cctgccaaat gtccgtcgtt ccatggccgt ctccagttat tctgttgcct caatacagcc
     2521 cagccgcgcg ggctctgact ttcgccatcg caaccagcaa atcgagcgtc ggcgtggcaa
     2581 cgccggccgc gcgcgcaagt tccagtggcg catcaaaaat gccgtcgatc tccatcggcc
     2641 ggccaagctc caggtcctgc aggatgctcg gcttatggtc cagattgcgg ccgtgctcaa
     2701 tgcgcttggc gtgattgctg ctggggtgcg ccccaagtgc atttgccacg gcctgagcct
     2761 cctccatcac ccggcgcgcc gcgtcgacgc atgtcggctc ttggtacagg ttcttgatgc
     2821 tgacctgcgt cagcacagcg agcggcccgt taccgagatt gcccagcaac ttgttccaga
     2881 tctcatcgcg gatcttcgta ctgacgatcg ccgtcatgcc gcccgcattg atgtgcgacg
     2941 cgatggcctg cacacgttgc gattcacgat catccggctc gccaagcacg acgcgaggtt
     3001 cggcgttctc aacttcaatg acaccgggcg caatgacggc actggcggca tagacgacgc
     3061 cggccatcgc gcgttccgga ccaaccgtac gccacagcgc gtcatccgga tcgatcttcg
     3121 gcagacggtg gccttcaagc tttccgccaa ttccatggaa gtagaaccag ggaatgccgt
     3181 tcatcacgaa tgcaaccgaa gtgtccgaat tcagcaatgg cccgatgccg gcggcaaccg
     3241 cgggaagcgc tggcgccttc accgtgacga tcaccgcgtc ctgcggaccg agcgccttcg
     3301 catcatcgct cgcctgcacg cggacattgt gctgaccgtc gctggcatgc accgtcaggc
     3361 cgtcgcgctg gatcgccgcc agatgcggcc cgcgcgcaag caccgacacc tccgcacccc
     3421 ctcgcgcgag gcgcacggcc agatggccgc cgatcgcacc ggcgccatac acgcaaatcc
     3481 gcatcccagc ctcctcaggt ttgcggcaag tctggcgatt gcggctgcgc ggcgcaacca
     3541 gtcgtccacg gatgcaccgg cagcgcgctg cgcccagcta cctcacaacg atctcgccga
     3601 ccattccatc ccagtcatga tcagcgcagg tcaggtgata agtccctgcc ttcagaggct
     3661 ccaggtagat atcggctgac ccacccggct gcaccacaac ttcctggccg ccattcgcca
     3721 gttgccctgg gtctcgcatc acggcagccg cgaaaaactc cggcgcggtg aactcatgca
     3781 aatccttccc gtgattctcc aggcgcaaca gatactgcgt gccgcgttcg aatgtcagat
     3841 gatccggctg gaaccggtca tccaccatca ccaccgtcat gggctgcgcg tgcgaccagt
     3901 gaaccgctgg cgccgccgcc agcaagacca ggacagccgg cagcagcccc ttcaagcggc
     3961 aactgtcggc aagccaacct gccggcgcac atcgttgatg atctgcagca cgcgctggcg
     4021 atacggcagg tactgtacgc cgaactcctg cactaggcgc gtgttgtcga tcagatagtt
     4081 gcccgacgtc gccttgcccc caacctcatg ctcgaacgtc acgcgtgcat cgggcaggaa
     4141 ttcccgcacc agatcagcaa tttcacccag actgatcgcg acgccacccg agttgtagac
     4201 gccgtgcttt ggcttatcag ccagcagcac acgcacgaac acctcggcga tgtcgtccac
     4261 atggatcgga caacgcatcg catccttgta tgggaaggag atcggcttgc cctgcgccgg
     4321 ctgggtgatg atgttcacat gatccaccga gccacgcacc ttgtctggcc ccgtcacgtt
     4381 ggccggccgt accgcagtga tcgtcatgcc gaatttctcg ttgtaatcct gcgcctgaaa
     4441 ttcgttgaag atcttatgca tcgcatactg cacgtcgccg tgcctgaaat cgtcctcggt
     4501 gatcatccga tcaccatagt gcttctgcat gccgttcacc gccagagaac tggcgaacac
     4561 cacgcggttc acccctgcca ggcgcgctgc ctcgaagcag ttgtccatac cgacgatgtt
     4621 cagcttggtg gcgatgtgcg gcgcatgatc gctgcccagg aagtaggaca ggttgatgat
     4681 gcggctcggc ttcgcactgc tcgtagcggc catcacgtcg tcgaactgcg tcacatcgcc
     4741 acgcatcaca cgcacctgct tgcccagatc gtcgaacgag gcagcacccg gattgatgtc
     4801 catgcagacg atctcttccc cgcgcgccgc aagcagacgg ataacgcgcg tgccgatgaa
     4861 ccccgtgcca ccgatgacga agatggacat attgatttcc tcccaaggcc ttggtgtggc
     4921 agactggcac gatgagaccc caggcggaag cgaagatcgt gcggcttgcc gaggccgaaa
     4981 cagtatcgtt cggtccgttg gcagactacg cgcgactgac cggtgatgat ggcctgccgt
     5041 tcttcaccgg cgtgcagaca tgcaagcccg gctaccagac gccgctgcat tggcacccct
     5101 atgtcgagtg cctcttcatc ctggaaggca cgatggaagc ctggctggag ggccaggacg
     5161 acaaaccgtc ccggcttggc cccggcgaca tgatcgcgct gcctgcctgc acgccgcatg
     5221 tgttccgcaa cgcgggcgaa ggaacgctcc ggctgcttgg catccacggc tcaccgacgc
     5281 gcatcgtgca tcggttggaa gagcagtccc cctagacctg atcgttccgc gtcagccgct
     5341 ggacgacgaa atccgtatcc atcacatcgc caaagatcgc gtagaaggcg accagcgagg
     5401 ccgtgtgttc ctccatgttc atcgccgcat tggcgtcact gaccatgatc gtcttgaagt
     5461 tcgtcatcat cgcgtcgcgc gcagatgact cgcagcaaac attggtgacg gtgccagtga
     5521 tcaataccgt atcaaagccg cgcgcccgca gccgataagg caactcacag gtcccaggca
     5581 tgaacgcact gaaccggtac ttgcggacaa tctcatcctc cggccggaca tccagctcag
     5641 gccacagttc ctggcccttg ctcccctcgc tcatcgcttc gatccggcgc ccacgagccg
     5701 ccggcgtcaa catctcctgc gcatggctcc actcaagcgc actgcgttca tcgaacgtgt
     5761 tcttgatcca gaacacgccg ccacccacgc cgcgcaccac cgatgcaagc cggttcaccg
     5821 caggcacgat gtcgcgcgcc atcgggcaca cagcgtggcc gatctcgtcg tccatgaaag
     5881 cgttctgcag atcgacgacc accagtgccg tacgcttcgg atcaagttcc gcgtgcggat
     5941 gcggtgtgcc tgtgcgcgcg ataacccgct gcatgacccc gtcgggaacc tcgaaccagt
     6001 tcatccgtcc gcctccacgc caagtttcgc cagctcagca ataatggcgc gccgccgggc
     6061 atcgagttcg tcgatcgaca ggcgctgcag ccgcccgagg aattccggcg aagcggaaaa
     6121 tccgctatag tgtgtggacg cagccccttc gctcgacatg cggatcgaag catcgccgga
     6181 cagcttgatg aacacctcga agtccgccag ctctgcccgt tcggcatggg cgatcagttc
     6241 tttcatcccg ttcaactcgg caatcagatg gttcacccgg tcgatgtcct ggatcttcat
     6301 gcaatatcgc ccaccgcccc gatgcgcgta ccgaaccgca tcacggccct ttggtcaaca
     6361 tcgtgccgtg gccaggatga cgccggctcg ccagtgcgga cgaatgcaat ccacgcctgg
     6421 cgcatcgctt cggacaggtc cgccatctgc ttccgatcgc cgccggccag catcggcgca
     6481 tctgcccacg catcgaacgt gccgaacacg aacggcaact cgatgcagtg g
//
LOCUS       DATGEM010000366         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1170555, whole genome shotgun sequence.
ACCESSION   DATGEM010000366 DATGEM010000000
VERSION     DATGEM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170555"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1254
                     /locus_tag="VKQ27_11635"
     CDS             178..1254
                     /locus_tag="VKQ27_11635"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HLY89625.1"
                     /translation="MPRSYRIASIPADGIGPEVISAGLEVLDAVAARDGNFTLTVDHY
                     DWGSDRYRKHGAFMPADAMAWLRTADAIYFGAVGDPDIPDHVTLWGLRLPICQGLDQY
                     ANVRPTRILPGITSPLRDVGPGDLDWVIVRENSEGEYAGHGGRAHRGLPEEVATETAI
                     FTRRGVERIMRYAFDIARGRPRKKLTVVTKSNAQRHGMVFWDEIAALVSHDYPDVTWD
                     KELVDAMTMRMVRRPQTIDTVVATNLHADILSDLAAALAGSLGIAPTGNVDPERRGPS
                     MFEPIHGSAFDITGKGIANPVATFWTGAMMLEHLGEKPAADLLMRAVEQVCAAGIMTP
                     DLGGKATTAEVTRAVCEAVRGLNR"
     gene            1254..>2302
                     /locus_tag="VKQ27_11640"
     CDS             1254..>2302
                     /locus_tag="VKQ27_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89626.1"
                     /translation="MDEDHLLEAARALLPALRDRAAATNAARDVPPETIADYHSANIL
                     RIIQPRRFGGLQLSFSLFSRIVETLTEACPSSAWVYAVLGEHQWLLASYPEQAQIDIW
                     GDSPRALASSSLAPRSTAERIAGGWRLSGTFPFSSGSTHASWAIIGAFLGKPGDPAAV
                     AYLLVPLGDIEIIDDWHVLGLRGTGSRSLKLHDAFIPDHRVVLTSDLFAGTVPGARIH
                     TDYPLVRAPRGLMVNYSLPPVAIALGNRALAIALETLRGRVSRGLTTMAISEYVQITI
                     AEAAAMIDAAALTMHHGREVAEARIADPTPIPSEYVARTRRNMTHAQHQVQAAVEKLI
                     EVCGARSVYDADPLAA"
BASE COUNT          392 a          875 c          674 g          361 t
ORIGIN      
        1 cgcaattggt tgcctacgag gcacgacttc aaaatttgct gaatgcgagg ccgctcgttg
       61 ccaggcgacg acggccactc gatttggacg cagacccgcc gtggcgtgcg agcatttggt
      121 gatccctcgc ccgcgcgcta ccactcgcag aactatcgcc aaccgggaac cacgagaatg
      181 ccccgctcct accgcatcgc ctccatcccc gccgacggca ttgggcccga ggtcatcagc
      241 gccggtctcg aagtgctgga tgccgttgcc gcgcgcgacg gcaatttcac cctcacggtc
      301 gaccactacg attggggctc tgaccgctac cgcaagcacg gcgccttcat gccggccgac
      361 gcgatggcct ggctgcgcac ggcagatgcg atctatttcg gcgccgtcgg cgatcccgac
      421 atccccgacc acgtcacgct ctggggcctg cgcctgccca tctgccaggg ccttgaccag
      481 tacgccaacg tgcgccccac gcgcatcctt cccggcatca cctcgccgct gcgcgacgtc
      541 gggccaggcg acctcgactg ggtcatcgtc cgcgagaaca gcgaaggcga gtatgccggc
      601 cacggcggcc gcgcccatcg cggcctgccc gaggaagtcg ccaccgagac cgccatcttc
      661 actcgccgcg gtgtcgaacg gatcatgcgc tacgccttcg atatcgcccg cggccgtccc
      721 cgcaaaaagc tgaccgtcgt caccaagtcg aacgcgcagc gtcacggcat ggtgttctgg
      781 gacgagatcg cggcccttgt ctcccacgac tatcccgacg tgacctggga caaggaactg
      841 gtcgacgcaa tgaccatgcg catggtgcgc cggccgcaga cgatagatac agttgtcgcc
      901 acgaacctgc acgccgacat cctgtccgac ctcgccgccg ctctggccgg ttcgctcggt
      961 atcgccccca ccggcaacgt cgatccagaa cgccgcggcc cgtcgatgtt cgaaccgatc
     1021 cacggcagcg ccttcgacat taccggcaag ggcatcgcca atccggttgc cacgttctgg
     1081 accggcgcaa tgatgctgga acatctcggc gaaaagcccg cggcggacct gctgatgcgg
     1141 gcggtggagc aggtctgcgc cgccggcatc atgacccccg acctcggcgg caaggccact
     1201 acggccgaag tgacgcgcgc ggtctgtgag gcagtgcgcg gcctgaaccg ctgatggacg
     1261 aggaccactt actcgaggcg gcgcgggcgc tgctgcccgc gctgcgagac cgtgccgcgg
     1321 cgaccaacgc cgcgcgcgac gtgccacctg agacgatcgc cgactaccac agcgccaaca
     1381 tcctgcgcat catccagccc cgccgtttcg gcggtctcca gctcagcttc tcgttatttt
     1441 cccggatcgt cgagaccctg accgaagcct gtccttcctc cgcctgggtc tacgcggtgc
     1501 tgggcgaaca ccaatggctg ctcgcctcct atcccgaaca ggcgcagatc gacatctggg
     1561 gcgacagtcc acgcgcgctc gcctcctcct cgctcgctcc acgatccacc gccgaacgca
     1621 tcgccggcgg ctggcgtctc agcggcacat tccccttctc gagcggcagc acgcacgcaa
     1681 gctgggccat catcggtgcc ttcctcggca agccaggcga tcccgccgcc gtcgcctatc
     1741 ttctcgtccc cctcggcgac atcgagatca tcgacgattg gcacgtgctc gggcttcgcg
     1801 gcaccggcag ccgctcactg aagctgcacg acgcgttcat ccccgaccat cgcgtcgtcc
     1861 tgacatcgga cctcttcgcc ggcacagtcc ccggcgccag gatccacacg gattacccgc
     1921 tggtccgcgc cccccgtggt ctgatggtga actactcgct gccgccggtc gcgatcgcgc
     1981 tcggcaaccg ggccctcgcc atcgcattgg aaaccctgcg cggccgcgtc tcccgcggcc
     2041 tcaccacaat ggccatcagt gaatacgtgc aaataacgat cgccgaagcc gccgcgatga
     2101 tcgacgcggc agcactcacg atgcaccacg gccgcgaagt cgccgaagcg cgcatcgccg
     2161 acccaacgcc catcccatcg gaatacgtcg cccgcacccg ccgcaacatg acccacgcac
     2221 agcaccaggt gcaggctgcg gtggagaagc tgatcgaggt ctgcggcgcg cgctcggtgt
     2281 acgacgccga cccgctggcc gc
//
LOCUS       DATGEM010000367        16290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_452261, whole genome shotgun sequence.
ACCESSION   DATGEM010000367 DATGEM010000000
VERSION     DATGEM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 16290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16290
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452261"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VKQ27_11645"
     CDS             <1..551
                     /locus_tag="VKQ27_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY89627.1"
                     /translation="AVQSMVDQATKAFGSVDISVSNASLRLHTPFLDISLEEWQRIIN
                     SNLNASFYLARAVIPGMKQNGWGRIIHISGRDGFMPIPNRVHNITCKAGVYAMAKALA
                     LEFGPHGITANTVSPGLIDTTRDWTQYPQFRNGYGERIKEIPVRRAGHVDEIAAACAY
                     LASDAAGFVTGQVIHVNGGDVM"
     gene            668..1225
                     /locus_tag="VKQ27_11650"
     CDS             668..1225
                     /locus_tag="VKQ27_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLY89628.1"
                     /translation="MHNGRQFMCRAAIAALAGWPLASRAMAANPADEGRQAAALLRAE
                     FVDATGGLHRLSELTRPLLLVDLWAAWCSGCIEELPTIDRLARQLGPESIDVVLIRHE
                     MNWQDDTVFARKNRLPFRFWRLSPHSAVLTEAAFGMESDRFGLPQSLVFAGRARTLVQ
                     SELGSQDWCAPPQLRRVRAWLAAAS"
     gene            complement(2279..3433)
                     /locus_tag="VKQ27_11655"
     CDS             complement(2279..3433)
                     /locus_tag="VKQ27_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89629.1"
                     /translation="MTETRPGRRSPAREQASTLIAFFVAGFAMASFAPLVPFVKARAG
                     IGEGALGLLLLCLGVGSLVTMFLAGALAARFGCRRVILASGVPLCLCLPLLGTLSDPL
                     ALGVTLLVFGGAIGSIDVVMNIQAIIVERESGRAMMSGFHGLFSVGGIAGSVCMTAAL
                     GAGASPIDATMGGVAVIIAALACAAPNLLAHASKHRPTVFAVPRGIVLFIGVLCFIAF
                     QAEGAVLDWSGVYLSGTEGMATHYAGLGYVAFTVTMTVGRLTGDRIVERFGGTRVILL
                     GGLCSASGFALTSLVSNWQPALFGFALIGLGCSNIVPVLFTRIGRQTLMPEHIAVSAI
                     STMGYSGILTGPALIGLVAQLSSLSLAFLIMAALQLGVAASGRFTSHRHR"
     gene            complement(3450..6089)
                     /gene="alaS"
                     /locus_tag="VKQ27_11660"
     CDS             complement(3450..6089)
                     /gene="alaS"
                     /locus_tag="VKQ27_11660"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLY89630.1"
                     /translation="MASSNDIRATFLDYFARNGHQVVESSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGQERRPYVRATTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEKAIPYAWELITKDYGLPKDRLLVTVYAEDDDAAAIWKKVTGFPDEKIIRIPTDANY
                     WRMGETGPCGPCSEISYDHGAEIPGGPPGSPDEDGDRFIEIWNLVFMQFEEGPPGTRV
                     PLPRPSIDTGMGLERIAAILQGKHDNYDIDIFRALILASAEATGTEPDGPHRVDHRVV
                     GDHLRSCSFLIADGVLPSNDGRGYVLRRIMRRAMRHAHMMGARDPLIYRLVPTLVRQM
                     GSAYPELARAEPLITETLLLEETRFRQMLERGLHLLNEETARLGDRQPLSGAVAFRLY
                     DTFGFPLDLTQDALREQGREVDLAGFETAMDEQRTRARAAWAGSGEAATERVWFELKE
                     SVGASEFLGYSTETAEATIVALVVNGAPVERAAAGSEVALVLNQTPFYGESGGQVGDT
                     GTITGPDGLRITVSDTQKKLGDLFVHLGRIESGTAQVGTAVLAEVDHARRTTVRAHHS
                     ATHLLHEALRRRLGTHVTQKGSLNAPDRLRFDVSQPRPMTADDIAWVETEVNARIREN
                     TEVTTRLMTPDAAVELGAMALFGEKYGEEVRVVSMGASEGNKAAYSIELCGGTHVRRT
                     GDIGYFKILSEGAVAAGVRRIEAVTGAYAEAVVTEMQHRLNDISGALRASPAEAPERV
                     AAIQDTVRRLERQVAELQRKLATGGSAAEIEDVNGVKLAARNLGEVPARDLKGLAEAI
                     GKQLGTGVVALVSTAEGKASIVVGVSQDLTGRFNAVELVRAASAAVGGKGGGGRPDMA
                     QAGGPDGGRAEEALAAVRGALAV"
     gene            6231..6512
                     /locus_tag="VKQ27_11665"
     CDS             6231..6512
                     /locus_tag="VKQ27_11665"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLY89631.1"
                     /translation="MIRSFADRNAEALFQGRFARRLGADIQRAAQRKLRQIHDAEQLT
                     DLAAPPGNRLEPLKGRRAGQHSIRINDRWRICIVWCDGGAEQVEIVGYH"
     gene            6524..6817
                     /locus_tag="VKQ27_11670"
     CDS             6524..6817
                     /locus_tag="VKQ27_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HLY89632.1"
                     /translation="MRELPPIHPGEQLREEFMKPLGLTAYRLAKDMGVPVTRIQAIIA
                     LKRDITGDTALRLGRYFNTTPEFWLNMQRDYELERAAEALGERLANEVKPRAA"
     gene            complement(6821..7783)
                     /locus_tag="VKQ27_11675"
     CDS             complement(6821..7783)
                     /locus_tag="VKQ27_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY89633.1"
                     /translation="MDSVDFLPADEIERALEDRLGRFHARQRRGIEIDHEAQKFGQGL
                     TFFHIENLPVLDAAIAAVVRLSGAWPRGRANAARVRLRHNELLSERLPGEFDGFRILH
                     LTDLHADMSEPAMMEAARLVGELQYDLCVLTGDYRGRTFGPYHRSLTNMLRLREALRG
                     DIYGVLGNHDTVLMLPDLERMGIRMLMNEHLTIERGGSAIFLAGIDDAHFYRVDNIEK
                     VAAAMPHDMFSVLLSHTPEIYRQASHAGFDLLLAGHTHGGQICLPGGIPVILASKLPR
                     SLGKGAWNYRGMSGYTSVGAGACGVPVRFNCPPEVTLHTLRKAR"
     gene            complement(7856..9178)
                     /gene="hslU"
                     /locus_tag="VKQ27_11680"
     CDS             complement(7856..9178)
                     /gene="hslU"
                     /locus_tag="VKQ27_11680"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HLY89634.1"
                     /translation="MEVPTYSPREIVSELDRFIVGQNDAKRAVAIALRNRWRRQQLPE
                     AMREEVVPKNILMIGPTGCGKTEIARRLAKLAQAPFLKVEATKFTEVGYVGRDVESIA
                     RDLVEASINMLRESSRKEVQAQAELAAEERIITALVGEGAAADTRSKFRRMLRNGEFE
                     QKEIEVDVSQSSGTPIGQLDIPGMPPGQVINLGEMMKGMFGGRPTQKRKLTVEAARRI
                     LQQEEADQLLDNDRLTKDAVSHAENNGIVFIDEIDKVAARTMEGGFRGGDVSREGVQR
                     DLLPLIEGTTVSTKYGQVKTDHVLFIASGAFHLTKPSDLLPELQGRLPIRVELAGLSR
                     DDLRRILTEPEHSLIKQSSALLATEKVTLNFTDGAVDALAELAADINDRVENIGARRL
                     HTVLERLLEEISFTASDKAGETITVDAAYVNERVAPLAQKGDLSRFIL"
     gene            complement(9181..9735)
                     /gene="hslV"
                     /locus_tag="VKQ27_11685"
     CDS             complement(9181..9735)
                     /gene="hslV"
                     /locus_tag="VKQ27_11685"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLY89635.1"
                     /translation="MIAAADPTGWHGTTILCVRRDGHVTMAGDGQVSMGQTIVKGNAR
                     KVRRIGTGGTVLAGFAGATADAFTLLERLEGKLERFPGQLERACVDLAKDWRTDRYLR
                     RLEAMMAVADKDRSFTLTGTGDVLEPEDGVIAIGSGGNYALAAARALIAVDNLSAEEI
                     ARRAMKIAAEICVYTNNNVIIETI"
     gene            complement(9797..10732)
                     /locus_tag="VKQ27_11690"
     CDS             complement(9797..10732)
                     /locus_tag="VKQ27_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLY89636.1"
                     /translation="MQSPPVLTERVPPHAFFLVSAVFHYLGPSFAVLLFVRLEPTGVA
                     WLRIATAGAAFALWRRPWRRLAMLSSDDRMVIAGLGLVLAAMNIVFYQAIARLPLATV
                     GAIEFAGPIAIAAWGTRTMRNLVALALATAGVIALTEVRLAAQPVGYLFAFANCGLFM
                     LYILLGHRIAASAPAVSGIDRLALAMLVATVAALPVGLQQALPAFTDAPLMVAAVGVG
                     ISSSVIPYVCDQLAMRRLPRASFALLLSLLPASAAVIGFLVLRQAPSLPDMVGILLVV
                     AGVGLHRAEATGAARSKPGPAAATFAESAIELRRG"
     gene            10824..11306
                     /locus_tag="VKQ27_11695"
     CDS             10824..11306
                     /locus_tag="VKQ27_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLY89637.1"
                     /translation="MNLQYADLLRDPRNIELLHLLREDPRIGTAELARRVGMSAPAVR
                     ERVLRLEEAGIIRGYSVEIDPAALGYPICAYVRIRPAPGRLPQVADLARRMPQVVECH
                     RVTGEDCFVVKLYLPALEQLDEVIDQFLAHGQTTTSLVQSTPVPLRDLPLPDSPKIAT
                     "
     gene            11408..12805
                     /locus_tag="VKQ27_11700"
     CDS             11408..12805
                     /locus_tag="VKQ27_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89638.1"
                     /translation="MATQAELAARLDRLPMTKHIWYLVTLISLGGCFEIYDLFLTAYI
                     APGLNKAGYFTAESLGIFNVLGPLHVAGIGTFVFALFAGLFVGAICLGHVADHYGRRT
                     VFTFSLVWYSITTIIMAFQTSGFGVDLWRFIAGIGIGVELVTIDTYVAEVVPRSHRGR
                     AFAFNQFFQFLAVPIMAFIAYLLVPTAPFGFEGWRWVVLIGSAGAIVIWFIRLGLPES
                     PRWLARHNRLAEAEQVIASIEAGVAADIGGTLPAPGVAEEVEQGEGSFAEIWQPPYDR
                     RAIILSVFNFFQTFGYYGFAAWVPTLLIAKGITITTSLLYAFVIAIANPVGPLLGTLI
                     ADRMERKWQVCLGAIGIGVFGMLFADATDHTLLIVFGVLVTLCNNLMSYSFHNYQSEV
                     FPTRIRSRAVGFVYSWSRLSAALSGLVVAYLLNLGGVSAVFTFIAFSMFIVVISIGGF
                     GPRTRGLALEAISNA"
     gene            complement(12973..14037)
                     /locus_tag="VKQ27_11705"
     CDS             complement(12973..14037)
                     /locus_tag="VKQ27_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89639.1"
                     /translation="MKFGFTVMADIDEIGFFNHAESLGYDSVWVADSQMLFSDCYAVL
                     ALAARATCTLRIGPGTAICGTRIPPVQVAAMATLNRLAPGRVFLGIGTGNTAMRSMGQ
                     RPMRIAVFADYLRVLSALLRGEVVDYAFNGAAKPISMLMHEWKYMSLEPKIPLYVSGF
                     GPRAMGLAGEYGDGLVFAIPPRGVAVAEALGHVRQGAGRAGRDLTGFRNSALTNVAVL
                     QPGEAADSDRIKAAIGPNVMASVYYFYDEVHEKTIDPPPFLERIWKPYCALVEATPPE
                     QRHFRTHEFHYTRLHPGEAELIDAQLIRDTCLVGTPDELIERIRELERDGLQELTFAT
                     GNASKWRLAEDFARLVMARL"
     gene            complement(14106..14768)
                     /locus_tag="VKQ27_11710"
     CDS             complement(14106..14768)
                     /locus_tag="VKQ27_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY89640.1"
                     /translation="MKLKILIADDHALLRQGMRQLLGDEHPEYEFAEADGLDATLDAL
                     AAGGADLLLIDLGMPGMAGAESLRALREAHPATRVAVITGSDDRGTILECLGAGVHGY
                     ILKSSSVDEIQRAISTILAGGVYVAPSVTQLDPARAPPVAPVVEALAGVPDMQQEIPR
                     FTHRQLDVLRLLAEGRSTKDIARTLELGPGTVKIHLAAIFRALNAHNRTEAVVLASRL
                     KL"
     gene            complement(14976..15941)
                     /locus_tag="VKQ27_11715"
     CDS             complement(14976..15941)
                     /locus_tag="VKQ27_11715"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF021205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HLY89641.1"
                     /translation="MTRALFVLAFVFGIAGCAANDPYGPNAQGGCPLIRETTMPIEMH
                     RNMLFVRVTIAGQPVKLLVDTGAEKTLLTEQTVERLHLPRDYTHATRTFGIGNATAAW
                     DALLPDGMVLGGVHFPVDRVTVGHFDIADVGGDAADGLLGADILLAFDLDLDLPKQQL
                     TLYRARRQCPDATPPWPQPYIGLTGVATHRDRLMVPFELDGVPGLAVVDTGAQLSSIS
                     ERMAERMGVIPSVLSRDRTVVAHGAAPNQVEVRLHRFRELRVGPAVMEAPALPVVPMT
                     SGMGDALVGADFLRGRRTWLSFSTHRIFVTPLEGTPLLAITNTGE"
     gene            complement(15938..>16290)
                     /locus_tag="VKQ27_11720"
     CDS             complement(15938..>16290)
                     /locus_tag="VKQ27_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89642.1"
                     /translation="LALPELSHVFDLLPKLPRSTSHTHERLRQPSVVRADALHLLFPP
                     AGSVLSADGPVTIRVMGGRRPLTFMVDGAPLPTDRIRRDVAWQPAGPGFYHLTVLDAD
                     GTAVRANVQVRAIP"
BASE COUNT         2896 a         5293 c         5176 g         2925 t
ORIGIN      
        1 cggctgtgca atcgatggtc gatcaggcga ccaaggcatt cggcagtgtc gatatctcgg
       61 tctccaatgc gtcgctgcgc ctgcacacgc cgttcctcga catctcgctg gaggaatggc
      121 agcgcatcat caactccaac ctcaatgcat cgttctacct ggcgcgtgcg gtcatccccg
      181 gcatgaagca gaacggctgg gggcgcatca tccacatctc cggccgcgac ggcttcatgc
      241 cgatacccaa ccgcgtccac aacatcacct gcaaggcggg cgtatatgca atggcaaagg
      301 cattggcgct cgagttcggt ccgcacggca tcacggcgaa caccgtctca cccggcctga
      361 tcgatacgac gcgtgactgg acgcagtacc cgcagttccg caacggatat ggcgagcgga
      421 tcaaggaaat cccggtacgc cgcgccggcc atgtcgatga gatcgcggcc gcctgtgcct
      481 acctcgcgag tgacgccgcc ggcttcgtca ccggccaggt catccacgtc aacggcggcg
      541 atgtgatgta gatcctggct cctgccggcg gcgatccgat ggaaacgttt gcgcacccgc
      601 gcgtgcaacg acaatgtaaa gacgacgagg cactctgcgc gcaggctgaa acgacggagc
      661 cttttacgtg cacaacgggc ggcagttcat gtgccgcgcg gcgatcgccg cactggctgg
      721 gtggccgctc gcttcccgcg ccatggcagc gaatccagct gacgagggac gccaggcggc
      781 cgctttgctg cgcgcggagt ttgtcgatgc caccggtggt ttgcatcgac tgagcgaatt
      841 gacccgcccg ctgctgctgg tcgatctctg ggcggcctgg tgttcaggat gcatcgagga
      901 actgccgacg atcgaccgcc tggccaggca acttggacct gagagtattg atgtcgttct
      961 gatcaggcac gaaatgaact ggcaggacga cactgtgttc gcacgcaaga accggctccc
     1021 cttcaggttc tggcggttgt cgccgcattc ggcggttctg accgaggccg ccttcggcat
     1081 ggagtctgac cggttcgggc taccgcaatc cctggtcttc gccggccggg ctcggactct
     1141 ggttcagtca gagcttgggt cgcaggactg gtgcgcgccg ccgcagctcc ggcgtgtccg
     1201 tgcctggctg gccgcggcaa gctgacgcat caacagcagt acggccacgc cgccgacgag
     1261 gaccgagagg gagccgggtt cgggaaccga gggatcaggc ggcgagggat cggacggcga
     1321 accgggcgtg gtcggcgtga cggggtagta tgattcgggc gtgaagggcg gcagcacgtt
     1381 ggctacttca aacgcactgg gcgcgatcgc agggggcgat tcgggccagt tggactgcag
     1441 aaggccagtc tgcccaaacg acgaccaggg cgcaaagacc ggtgccggct gcatgtagcc
     1501 gggcggcgga gccggcactg gcggcgcgct tgccgggact ggcgcaagca gaccaccacc
     1561 cgcaaccgct attggtgctg ccttgcagac gagttcgacc caggtgtggg caggacgagc
     1621 gacggcgcga gccgcaccac gggcgacgtg atgcggcccc cggctaacaa agtgtcgcaa
     1681 cggatgcgcg gcgcgtgtca ggccgctcgc ggccgaggcg gcggtccgat gcacgatagt
     1741 ccaacaggcg cttgacattg gttgccggcc cccgccggcg cattcccgaa cgtgatagtt
     1801 gatccggtca catacgcgct cggacgtacc aataatacga ctataatcaa tgtctatgcg
     1861 aattgcaggg gattttcgcg catgagttgc cgcaccgcca cggcctcttg tcggggctcc
     1921 gcggatcgcc actgatgacg cgactgcgcg agccgcgtca tcacggtcga ttgcgccact
     1981 ttttcggacg tcccgcggct gcctcggccg tcgaccgcgg cccaggcggc gcgtatcagt
     2041 tcggccgctt caaggcaggc tgtcggagcc tccggtcact tctcggggtc agatattcta
     2101 ccatagattg cagatctgtg ttgcagcttc gccacctgcc gctccgggcg ccggcgctga
     2161 cctgcatagc gacgacgcac tgcggcgcct cggcagcatt cgcacacagc gggcctggca
     2221 ttcgttcagc cggtgctcct tcgcggcgac aacggcttcg tcaccagacg gacccacgtt
     2281 aacggtgccg atgtgatgtg aaccgcccgc tggcggccac gccaagctgc agcgctgcca
     2341 tgatcaggaa ggccaaagac aggctggata gctgtgcgac caggccgatc agtgccggcc
     2401 cggtcaggat gcctgaatag cccatcgtgc tgatcgccga gacggcgata tgttccggca
     2461 taagggtctg ccgcccgatc cgggtgaaca gcaccggcac gatgttggag cagccgagcc
     2521 cgatcagcgc gaagccgaac agcgccggct gccagttcga taccagactg gtcaacgcga
     2581 agccgcttgc cgagcacagc ccgcccaaca ggatgaccct cgtgccgcca aaccgctcga
     2641 cgatacgatc gccggtcagc cggccgaccg tcatggtgac cgtgaatgcc acgtagccga
     2701 gaccggcgta gtgggtggcc atcccctcgg tcccgctgag gtatacgcca ctccagtcga
     2761 gcaccgcgcc ttccgcctgg aatgcgatga agcacaacac accgatgaac aacacgatgc
     2821 ctcgcggcac ggcgaaaacc gtcggcctgt gtttgctggc atgggccagg agatttggtg
     2881 ccgcgcacgc cagtgcggcg atgatgacgg cgactccacc catcgttgca tcgattggcg
     2941 atgcccctgc gccgagcgcg gctgtcatgc agaccgagcc ggcgatgccg ccaacgctga
     3001 acagcccatg gaagccggac atcatggccc ggccactctc tcgctcaaca atgatggcct
     3061 ggatgttcat gacgacatcg atggagccga ttgccccacc gaacaccagc agcgtcaccc
     3121 caagcgcaag cggatcggag agagtcccca gaagcggcag gcacaggcag agcggcacgc
     3181 cggacgccag tatgacccgc cggcagccga accgcgcggc cagggcgccc gccaggaaca
     3241 tggtgacgag cgagccgacg cccaggcaaa gcagcagcag cccgagtgca ccctcgccga
     3301 tgccggctct cgcctttacg aacggcacca gcggggcgaa cgacgccatg gcgaagcccg
     3361 cgacgaagaa tgcgatcagc gtcgaggcct gctcgcgtgc cgggctgcgc cggccgggtc
     3421 gtgtttcggt caagaccgaa ggcgagcggc tataccgcca atgcgcctct taccgcggcc
     3481 agtgcctctt cggcgcgccc gccatcgggt ccaccggcct gtgccatatc cggcctcccg
     3541 ccgccgccct tgccaccgac cgcagccgag gcggcgcgca cgagctccac cgcgttgaac
     3601 cggccggtca ggtcctgcga cacgcccaca acgatactgg ccttgccctc ggccgtggat
     3661 acaagcgcca ctacacccgt gccaagctgc ttgccgatcg cttcggccag gcccttgaga
     3721 tcgcgcgcgg ggacctcgcc caggttgcgc gcggccagtt tcacgccgtt cacatcctcg
     3781 atctctgcgg cgctgccgcc ggtcgccagc ttgcgctgca gctccgcgac ctgccgctcc
     3841 agccgccgca cggtgtcctg tatcgcggcg acacgctccg gtgcctcggc ggggctggca
     3901 cgcagcgccc ctgagatgtc gttcagccta tgctgcatct cggtcaccac cgcttcggcg
     3961 taagcaccgg tcaccgcctc gatacgacgc acacccgcgg ccaccgcgcc ctccgacagg
     4021 atcttgaagt agccgatgtc gccggtgcgc cgcacatgag tgccgccgca cagctcgatc
     4081 gaatacgctg ccttgttgcc ttcgctggcg cccatcgaaa cgacgcgcac ttcctcgccg
     4141 tacttctcgc cgaacagcgc catcgcaccg agttcgacag ccgcatcggg cgtcatcagc
     4201 cgcgtggtca cttcggtgtt ctcgcggatg cgtgcgttca cttcggtttc cacccaggcg
     4261 atgtcgtctg ccgtcatcgg acgaggctgc gacacatcga accgcagacg atccggcgcg
     4321 ttcaggctgc ccttctgcgt cacatgcgtg ccgagccgcc gccgcagggc ctcatgcagc
     4381 aggtgggttg ccgagtgatg cgcgcggacc gtggtgcgcc gcgcatgatc cacttccgcc
     4441 aggacggccg tgccaacctg cgccgtaccg ctctcgatgc ggccgagatg cacgaacaga
     4501 tcgccgagct tcttctgcgt gtcgctcacc gtgatgcgca ggccatccgg cccggtgatc
     4561 gtgccggtat cgccgacttg gccaccgctt tcgccgtaga acggcgtctg gttcagcacg
     4621 agagcaacct cgctgcccgc cgcggccctt tccaccggcg cgccattcac caccagcgcg
     4681 acaatcgtcg cctcggccgt ttccgttgag tagccgagga actcggatgc gccgacactc
     4741 tccttcagct cgaaccacac gcgctcggtc gcagcttcgc ccgagccggc ccaggcggcg
     4801 cgggcacgcg tccgttgctc gtccatcgcc gtctcgaagc cggcgaggtc cacctcgcga
     4861 ccctgctcgc gcagcgcatc ctgagtgaga tccagtggga agccaaaggt gtcatagagc
     4921 cggaacgcga cggcacccga aagcggctga cggtcgccga gccgcgcggt ctcctcattg
     4981 agcaagtgca ggcctcgctc gagcatctgc cggaaccgtg tctcttccag cagcagggtc
     5041 tcggtgatca gaggttcggc gcgcgcgagt tcaggatagg cgctgcccat ctgtcgcacc
     5101 agcgtcggca cgagccggta tatcagaggg tcgcgcgccc ccatcatgtg ggcatggcgc
     5161 attgcgcggc gcatgatccg gcgcaggacg tagccacgcc catcgttcga aggcagcacg
     5221 ccatctgcga tcagaaagga acaactgcgc agatggtcgc caacaacgcg atgatcgacg
     5281 cgatgcgggc cgtcgggctc ggtgcccgtg gcctcggcgc tggccaggat cagcgcgcgg
     5341 aatatgtcga tgtcgtagtt gtcgtgtttc ccctgcagaa tggcggcgat gcgctccaga
     5401 cccatgccgg tgtcgatcga gggccgtggc agcggcacgc gtgtgccggg tggaccttcc
     5461 tcgaactgca tgaacaccag gttccagatc tcgatgaagc ggtcgccgtc ctcgtccggt
     5521 gagcccggtg ggcccccagg aatttccgca ccatggtcgt aggagatctc gctgcacgga
     5581 ccgcaggggc cggtttcgcc catgcgccag taattggcgt cggtgggaat gcggatgatc
     5641 ttctcgtccg ggaagccggt cacctttttc cagatcgccg cggcatcgtc gtcctcggcg
     5701 taaaccgtga ccagcagtcg atcctttggc aggccataat ccttggtgat cagctcccag
     5761 gcgtacggga tcgccttctc tttgaaatag tcgccgaagc tgaagttgcc cagcatctcg
     5821 aagaaggtgt ggtggcgcgc ggtgtagccg acgttatcga ggtcgttgtg cttgccgccg
     5881 gcgcggacgc atttctgcgc cgtggtcgcc cgcacatagg ggcgcctttc ctggccggtg
     5941 aacacgttct tgaactgcac catgccggcg ttggtgaaca tcagcgttgg atcgttgcgc
     6001 ggcaccagcg gcgagctttc caccacctgg tgcccattgc gggcaaagta gtcgaggaag
     6061 gtggcgcgga tgtcattgct gctggccatg gcggtcagtg ggcttccaga gtgttggcga
     6121 agagctatga ggtagcgcgc agggggccaa atgtcaccgg tcagcggtcg ggataaccgg
     6181 cgcgcacccc gtacgcttga cgttcagaac gcggcacgta tacagggcga gtgattcgct
     6241 cgttcgccga tcgcaatgct gaagcgctat tccaaggccg tttcgcgcgg cgactgggcg
     6301 ccgatatcca acgggctgca cagcgtaagt tgcggcagat ccacgatgcg gaacagctga
     6361 cagacctggc ggctccgccg ggaaatcgcc tcgaaccgct gaaggggcgg cgggccggac
     6421 aacactccat ccgcatcaac gaccgttggc gaatttgcat tgtctggtgc gatggcggag
     6481 ctgagcaggt tgaaatcgtc ggctatcatt gaggaccacg gcaatgcgtg agcttccccc
     6541 aatccatccc ggcgaacagt tgcgggaaga gttcatgaaa ccgctgggcc tgacggccta
     6601 tcgactggca aaggacatgg gtgtcccggt aacgcgcatt caggcgatca tcgccctgaa
     6661 gcgagacatt accggcgaca ctgcgctgcg gctgggccgc tacttcaaca cgacgccgga
     6721 gttctggctg aacatgcaac gcgactacga actggaaaga gccgccgagg ctttgggcga
     6781 gcgtctggca aacgaggtga agcctcgtgc cgcgtaatcc tcagcgcgcc ttgcgaagcg
     6841 tatgcagtgt cacctcaggc gggcaattga accgcacggg aacaccacaa gcgccggcac
     6901 cgacggatgt gtagccggac atcccgcgat aattccacgc gcccttgccc agcgaccgcg
     6961 gcagcttcga cgcgagaata actgggatgc cgccaggcag acagatctgg ccgccatgcg
     7021 tatgccccgc cagcaacaag tcgaagccgg catgcgaggc ctgccgatag atttccggcg
     7081 tgtgcgacag caggacagag aacatatcgt gcggcatcgc ggccgcgacc ttctcgatgt
     7141 tgtccacccg gtagaaatgc gcgtcgtcaa ttcccgccag gaagatcgcg ctgcccccgc
     7201 gttcgatagt gaggtgctcg ttcatcagca tccggatgcc catgcgctcg aggtccggca
     7261 gcatcagcac ggtgtcatgg ttgccgagaa cgccgtagat gtccccgcgc aaggcctccc
     7321 gcaggcgcag catgttggtc agcgaacgat gatacgggcc gaaggtccgt ccgcgataat
     7381 cgcctgtcag gacacacagg tcatactgaa gctcgccgac gagccgtgcg gcttccatca
     7441 tcgccggctc gctcatgtcg gcgtgcaggt ccgtcagatg caggatcctg aagccgtcga
     7501 attcgccggg cagtcgttca gagagcagtt cgttgtggcg cagcctgacg cgcgctgcat
     7561 tggcacgacc gcgcggccat gcaccggaca gacgcacaac cgcagcaatg gcggcgtcga
     7621 ggaccggtag attctcgatg tgaaagaacg tcagcccctg gccgaacttt tgcgcctcgt
     7681 ggtcgatctc gatgccgcgc cgctgtcgcg cgtggaagcg gcccaggcga tcttccagcg
     7741 cacgttcgat ctcgtcggcg ggaaggaagt ctaccgagtc catgccgcct ccatgtgcgc
     7801 ggtcacaggg agattggcac caccccctct ccccgcagcc aacgtggtgg cgagcttaca
     7861 ggatgaaccg gctcaggtcg cccttctgcg ccagcggagc tacacgctcg ttcacatagg
     7921 cggcgtccac ggtgatcgtc tcgcccgcct tatcactggc ggtgaagctg atctcctcca
     7981 gcaaccgctc cagtacagtg tgcaggcggc gcgcgccgat gttctcgacc cggtcattga
     8041 tgtccgccgc cagttccgcc agtgcgtcca ccgcaccgtc ggtgaagttc agtgtcacct
     8101 tctcggtcgc cagaagcgct gacgactgct ttatcagcga atgttccggc tcggtcagga
     8161 tgcgccgcag atcgtcccgc gacagccctg ccaactccac gcggatcggc agacggccct
     8221 gcaattccgg cagcaggtca gatggcttcg tcaggtggaa cgcgcccgag gcgatgaaca
     8281 gcacatggtc ggtcttcacc tgcccatact tggttgagac cgtggtgccc tcgatcagcg
     8341 gcagcaggtc gcgctgcacg ccctcacggg acacgtcgcc gccgcggaag ccgccctcca
     8401 tggtgcgcgc cgccactttg tcgatctcgt cgatgaagac aatgccgttg ttctcggcgt
     8461 gcgacacggc atccttggtg agccggtcgt tgtcgagcaa ctgatcggct tcttcctgct
     8521 gcagaatgcg ccgtgctgcc tcgacggtca gcttgcgctt ctgtgtcggc cgcccgccga
     8581 acatgccttt catcatttcg ccgaggttga tcacctggcc gggcggcatc cccggaatgt
     8641 cgagctgacc gatcggcgtg ccgctggatt gcgacacgtc aacctcgatc tccttctgct
     8701 cgaattcgcc gttgcgcagc atgcggcgga atttgctgcg ggtgtccgcc gccgcaccct
     8761 caccgaccag cgcggtgatg atgcgctcct cggcagcgag ttcggcttgc gcctgcactt
     8821 ccttgcgcga gctctcgcgc agcatgttga tgctggcctc gaccaggtcg cgggcgatgc
     8881 tctccacgtc gcggccgaca tagccgactt cggtgaactt ggtcgcctcg accttcagaa
     8941 acggtgcctg ggcgagcttg gccagccggc gggcgatctc cgtcttgccg cagccggtcg
     9001 ggccaatcat taagatgttc ttgggaacga cttcttcgcg catcgcctcg ggcagctgct
     9061 gccgccgcca gcgattgcgc agcgcgattg ctaccgcgcg cttggcatcg ttctggccga
     9121 cgatgaagcg gtccagctca gagacgatct cgcggggtga gtaggtggga acttccatat
     9181 ctagatggtc tcgatgatca cgttgttgtt ggtatagacg catatttccg cggcaatctt
     9241 catcgcgcgg cgggcgatct cctcggcgga caggttgtcc accgcgatca gcgcgcgggc
     9301 cgcggccagc gcgtagttgc cgcccgatcc gatcgcgatc acgccgtcct ccggctccag
     9361 cacgtcgccg gtgccggtga gggtgaagct acggtccttg tcagccaccg ccatcattgc
     9421 ctccagcctg cgcagatagc ggtcggtgcg ccagtccttg gccagatcga cgcaggcgcg
     9481 ttcgagttgg ccgggaaagc gctcgagctt gccttccagc cgttcaagaa gggtaaacgc
     9541 atcggcagtg gcgcccgcga agcccgcgag cacagtgccg ccggtgccga tgcggcgcac
     9601 cttgcgggcg ttgcccttga cgatggtctg acccatcgag acctggccgt cgccggccat
     9661 agtgacgtgt ccatcccgcc tgacgcacag gatggttgtg ccgtgccacc cggtcgggtc
     9721 ggctgcggcg atcatggtgt tctgcatagc cccgctaaat ggcgccgaat gcgggggttc
     9781 acaaggacat ggacgatcag ccgcgtcgta gctcgattgc agattcggca aaggtcgcgg
     9841 cggctggccc aggtttgctt cgcgcagccc cggttgcctc cgcccgatgg aggccgacgc
     9901 cagccaccac gagcaggatg ccaaccatgt ccggcagcga cggcgcctgc cgcagtacca
     9961 gaaagccgat gacagcggcg ctcgcgggaa gcagtgagag cagaagcgcg aagctcgcgc
    10021 gcggcaggcg gcgcatggcc agttgatcac aaacgtaggg tatgaccgag gacgatatgc
    10081 ccacgccgac agccgctacc atcaggggtg catccgtgaa ggcgggcaga gcctgctgca
    10141 gccctaccgg cagggccgct accgtcgcca cgagcatcgc gagggccagt cggtcgatgc
    10201 cggatacggc cggtgcgctg gccgcgatcc ggtgtcccag caggatgtag agcatgaaca
    10261 gtccgcaatt ggcgaaggcg aacaggtagc ccacgggctg ggccgccagg cgcacttcgg
    10321 tcagcgcaat cacgccggcc gtcgccagcg ccagggctac caggttgcgc atggtccgcg
    10381 tgccccacgc tgcgattgcg atcggacccg caaattcgat cgctcccacg gtcgcaagcg
    10441 gcaagcgtgc gatggcctgg tagaacacga tattcatcgc tgccagcacg aggccgagac
    10501 cggcgatgac catccggtca tccgacgaga gcatggccag ccggcgccac ggccggcgcc
    10561 agagggcaaa cgcggcgccg gctgtcgcaa tccgcagcca cgcgacgcca gtcggctcca
    10621 gccggacaaa gagcagtacg gcgaacgacg gccccaggta atggaacacc gcactgacca
    10681 ggaagaaggc gtgcggcggg acacgctctg tcagaacagg tggcgactgc ataccagcag
    10741 aatcccatgt tgcgtcgggg cgataaatgc tgatacgaag ggaatatatg gaatatgccg
    10801 atgattcaga ggcaaattag agaatgaacc ttcaatacgc agacctgctg cgcgacccgc
    10861 gcaacatcga gctgctgcac ctactccggg aagacccccg gatcggaaca gcggagctgg
    10921 cgcgccgggt cggcatgtcg gcgcccgcgg tgcgcgagcg cgtgctgcgg ctggaggagg
    10981 ctggcatcat ccgcggctat tcggtggaga tcgatccagc agcgcttggc tacccgatct
    11041 gcgcctatgt gcgcatccgg cccgcgcctg gacggctgcc gcaagtggcc gacctcgcgc
    11101 gccgaatgcc gcaggtggtg gaatgccacc gcgtcacggg cgaggattgt ttcgtggtaa
    11161 aactgtatct gccagcgctc gagcaactcg acgaggtgat cgaccagttc ctcgcccacg
    11221 gccagacgac gacaagcctg gtgcagagca cgcccgtgcc actccgcgac ctgcctttgc
    11281 cggacagccc gaaaatcgca acgtagcgtt gcgcggcgtc cccttgacgc atggggaccc
    11341 ggagtccccg atgcttcgat gttcggtttc ttcaccgaat cgtactggct ccactgggga
    11401 gggaatgttg gcgacacaag cagaactcgc ggcgcggctc gaccggctgc caatgacgaa
    11461 acacatctgg tacctggtca cgctgatctc gctcggcggc tgctttgaga tttatgacct
    11521 gttcttgact gcctacatcg ctccgggcct gaacaaggcc ggttatttca ccgcggaatc
    11581 gctcggtatc ttcaacgtcc tcggtccgct ccacgtggct ggtatcggca ctttcgtgtt
    11641 tgcgctcttc gccgggttgt tcgttggcgc catctgcctg gggcatgtgg ccgatcacta
    11701 cggacgcagg acggtattca cattctcact ggtctggtac tcgatcacca cgatcatcat
    11761 ggcgttccag acttcaggct tcggtgtcga tctgtggcgg ttcatcgctg gcatcggtat
    11821 cggcgtggaa ctggtcacga tcgataccta cgtggctgaa gtggtgcctc gcagccatcg
    11881 cgggcgcgcc ttcgcgttca accagttctt ccagttcctc gctgtgccga tcatggcgtt
    11941 catcgcctat ctgctggtgc cgacggcgcc gtttggcttc gagggctggc gttgggtggt
    12001 gctcatcggc tcggccggtg cgatagttat ctggttcatt cggcttgggc tgccagagtc
    12061 gccgcgctgg ctggcgcgcc ataaccggct tgccgaggcg gagcaggtca tcgccagcat
    12121 tgaagccggc gtcgccgccg acataggcgg cacgctgcct gctcctggcg tggcggaaga
    12181 agtcgaacaa ggtgagggtt cgttcgccga gatctggcaa ccaccttacg atcggcgtgc
    12241 gatcatcctg tcggtgttca acttcttcca gaccttcggc tattacggtt tcgccgcctg
    12301 ggtgccgaca ttgctgattg ccaaaggcat cacgatcacc accagcctgc tctatgcctt
    12361 cgtcattgcc atcgccaatc ccgtcgggcc gctgctcggc acattgatcg ccgacaggat
    12421 ggagcggaaa tggcaggtct gtctgggcgc catcggcatt ggcgtgtttg gaatgctgtt
    12481 cgccgatgcg accgatcata cgctgctgat cgtgttcggc gtgctggtga cgctgtgcaa
    12541 caacctgatg tcgtacagct tccacaatta ccagtccgag gtgtttccca cccgcatccg
    12601 ctcccgtgcg gtggggttcg tgtattcgtg gagccgacta tcggctgccc tgtccggcct
    12661 ggtcgttgca tatctgctga atttgggtgg cgtcagcgcc gtgttcactt tcatcgcgtt
    12721 ctcaatgttc atcgtggtga tctccatcgg tggctttgga ccacggacgc ggggattggc
    12781 actggaagcc atatcgaacg cttgagtttg cgggagtagg ccagcctgtg agacagcccg
    12841 gcccgccacc tgcgccgatg cgcatgatca ctacagtcct tcggcaggag actgcgatgg
    12901 ctgtccatca ctatctccct cgtagcgccg gtcgaggacc accttcggcc ggcgtcgtaa
    12961 ttttcagcgc cgctatagcc tcgccatcac aagtcgcgcg aagtcttccg ccagtcgcca
    13021 cttgcttgcg ttacctgtgg caaatgtcag ctcctgcagg ccatcgcgtt cgagctctcg
    13081 gatacgctcg atcagttcgt cgggcgtgcc gaccagacag gtgtcgcgga tcaattgcgc
    13141 gtcgatcagt tcggcttcac cggggtgcag cctggtgtag tggaactcgt gtgtgcggaa
    13201 gtgccgttgc tctggcggcg ttgcttcgac cagtgcgcaa tagggtttcc agatccgttc
    13261 aaggaagggt ggcggatcga tcgtcttctc gtgcacttcg tcatagaagt aatatacact
    13321 tgccatgaca ttggggccga tcgccgcctt gatgcgatcg gagtctgctg cctcgccggg
    13381 ttgcagcacg gcgacgttgg tcagcgcgga gttacggaaa cctgttaggt cgcggccggc
    13441 acgaccggca ccctggcgca catgacccaa tgcttcggcg acagccacgc cacgtggcgg
    13501 aatggcgaac acgaggccgt cgccatactc acccgccaga cccatcgcac gcgggccgaa
    13561 ccccgagaca tagagcggga tctttggctc gaggctcatg tatttccatt cgtgcatcag
    13621 catgctgatc ggcttggccg cgccgttgaa cgcgtagtcc acgacctcgc cacgcaacag
    13681 cgccgacagc acccgcagat agtctgcgaa gacagcgatc cgcatcggcc gctgacccat
    13741 gctgcgcatc gcggtattgc cggtaccgat gccaaggaac acgcgtcccg gagccagccg
    13801 gttcagcgtt gccatggcgg ccacctgtac cggcgggatg cgtgtgccgc agatcgcggt
    13861 gccggggccg atgcgcagcg tgcatgtcgc gcgcgcggca agggccagca cggcataaca
    13921 gtccgagaac agcatctggc tgtcggcgac ccagacagaa tcgtagccca gggattcggc
    13981 gtggttgaag aagccgatct catcgatgtc ggccatgacg gtgaagccga atttcatgga
    14041 gttctcccgg tgtggcgttc gatggtggtg ccacacccgg aacagcagca gcaacgcccc
    14101 ttgtgctaca gcttcaacct ggatgccagc accaccgcct cggtccggtt atgcgcgttc
    14161 agcgcccgaa agatcgccgc gaggtggatc ttcaccgttc cgggcccaag ttccagcgtc
    14221 cgcgcaatgt ctttcgtgga acggccctcg gcgagcagcc gcagcacgtc gagctggcga
    14281 tgggtgaacc gcggtatctc ttgctgcatg tcggggacac cggccaatgc ctcgactacc
    14341 ggtgccactg gcggagcgcg ggccggatca agctgcgtca ccgacggtgc gacgtagacg
    14401 ccacctgcca gaatggtcga aatcgcccgc tggatttcgt caaccgatga cgacttcagg
    14461 atgtagccat gcacgcccgc gcccaggcac tcgaggatcg ttccgcgatc gtcggaaccc
    14521 gtgatcactg ccaccctggt ggccggatgc gcttcgcgta acgcacggag gctctccgcc
    14581 ccggccatcc ctggcattcc gagatcgatc agcagcaggt ccgccccacc cgcggcaagc
    14641 gcatcgagcg ttgcatccaa tccatctgct tcggcgaact cgtattcggg atgttcatcg
    14701 ccgaggagtt ggcgcatccc ctgtcgcagc agcgcgtggt cgtcggcgat caggatcttc
    14761 aacttcatcg cctggcccaa ttagcaggta tttgcttgca agatactatc cgcgtttcgt
    14821 tctggctgct tccgcacaac ccagttgaca tcaggccgag atggtcagca taccttggtt
    14881 tcctcaattc tttgcgatcg tccgtggaaa ttgtcgtctg ccactattgg gggggtagta
    14941 aaactcaatc gcagaaactg aacgtttatt cgccgctatt cccccgtatt ggtaatcgcc
    15001 aataatggtg tcccctcgag cggggtcacg aagatgcgat gcgtggagaa cgacagccag
    15061 gtgcggcgcc ccctcaggaa gtcggctcca accaaggcat cacccatgcc gctggtcatc
    15121 ggcaccacag ggagggccgg tgcctccatc acggccggtc cgacacggag ctcacgaaac
    15181 cggtggagcc gtacttccac ctggttgggc gccgcgccat gagccacgac ggtgcgatcg
    15241 cgcgagagca cgcttgggat cacgcccatc cgctccgcca tgcgctcact gatcgagctg
    15301 agctgcgctc cggtgtcgac gacggccagc ccggggacgc catccaactc aaagggcacc
    15361 atcagccgat cccggtgcgt cgcaacaccg gtgagcccga tatagggctg cggccatggc
    15421 ggtgtggcgt ccggacactg gcgccgggcg cgatacaggg tgagctgttg cttcggcaga
    15481 tcgagatcga gatcgaaggc caggagaatg tcggcaccga gcaagccatc cgcagcgtcg
    15541 ccaccgacat cggcaatgtc gaaatggcct accgtcacgc ggtcaaccgg gaaatgcacg
    15601 ccacccagca ccattccgtc cggcagcagc gcgtcccagg ctgcggtcgc gttgccgatg
    15661 ccaaaggtgc gcgttgcatg cgtgtagtca cgcggcagat gcagccgctc gaccgtttgc
    15721 tcggtcagca gggtcttttc ggccccggta tcgacgagca gctttaccgg ctggccggca
    15781 atcgtcaccc ggacgaacag catgttccga tgcatctcga ttggcatcgt ggtttcgcgg
    15841 atcagcgggc agccgccttg ggcattcggg ccatacgggt cattggcggc gcatccagcg
    15901 atgccaaaca cgaaagccag aacaaatagc gcgcgggtca tggaatggcg cgcacctgta
    15961 cgttggcgcg caccgcggtg ccgtcggcgt cgagcacggt cagatgatag aagccaggac
    16021 cggccggctg ccaggccaca tcccgccgga tgcggtcagt gggaagcggc gccccgtcca
    16081 ccatgaaggt caacggccgg cgtccgccca tcacgcggat ggtgaccggc ccgtcggcgg
    16141 acagaacgga gcctgcgggc gggaacagca ggtgaagcgc gtccgcgcgc acgacggatg
    16201 gttgcctcag ccgctcatgg gtatgcgagg tggagcgagg caatttcggc agcaaatcga
    16261 acacgtggga cagctctggc agtgctagcg
//
LOCUS       DATGEM010000368         3804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1424571, whole genome shotgun sequence.
ACCESSION   DATGEM010000368 DATGEM010000000
VERSION     DATGEM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424571"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..692
                     /locus_tag="VKQ27_11725"
     CDS             141..692
                     /locus_tag="VKQ27_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol phosphotransferase"
                     /protein_id="HLY89643.1"
                     /translation="MVARIVLLNGVGSAGKTSIAKALQSIAHVPLLHIKMDAFLDMLP
                     EAMVGHPDGLVFEPMELDGKPAVAIRSGPVADRAFRGMRHSVAALAAQGNDLVVDEVL
                     LGTKAREYAELLSPFRLYRVGVFAPLNVLEARERQRGDRLIGLARWQYDRVHQNMTYD
                     LELDTSRASPMTCAELIRDTFSL"
     gene            complement(682..1356)
                     /locus_tag="VKQ27_11730"
     CDS             complement(682..1356)
                     /locus_tag="VKQ27_11730"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HLY89644.1"
                     /translation="MPQHIGVVACSAEGASLCYRTICAEGARLLGPHSHPEVSMHTPS
                     LAEYVHWLDRDDWAGVGELMLASARKLATIGADFLICPDNTIHQALPLIVERSPLPWL
                     HIASAVAEEAATRGFRRVGLLGTRWLVTGPVYPSEYMRPDTEERAELNRIIMDELVCG
                     VFRPEAVAFHQRVIARMKQAGCDGVVLGCTEIPLIINDDNSPLPTLDSTRLLARAALR
                     RAVSTG"
     gene            complement(1393..2082)
                     /locus_tag="VKQ27_11735"
     CDS             complement(1393..2082)
                     /locus_tag="VKQ27_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89645.1"
                     /translation="MAKSLLLIGASRGLGHAMAEEFLKHGWNVVGTVRGEARTRLHEL
                     ADAATGRVDIETIDITVPEQIAALRSRLAERSFDMLFVNAGVTNNPDETIGEVTTEEF
                     VRVMVTNALSPMRVVEALHDLVIPDGTIGVMSSGQGSVTNNTTGMREVYRGSKAALNT
                     FMRSFAARHAEDKRAIVLMAPGWVRTDMGGSSGRLGIEESIPNVVRTLLAVQGKAGLQ
                     YLDYLGSTVPW"
     gene            complement(2234..>3804)
                     /locus_tag="VKQ27_11740"
     CDS             complement(2234..>3804)
                     /locus_tag="VKQ27_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89646.1"
                     /translation="LSDGGTGGGQLLLNGQPVNGDTSFTAAEFAKLTYQAGADGTQQN
                     LIVVAQTGKLLANGTLTQVIDSPAVAITANSAQTGSINAAGALVIKPTGAEGDVAGIA
                     SEAGILTGLGTGRPTVRTDGNFTAVAGDAFRIGDLFQASALSGQTLAGYRVALGNGSG
                     QLLLNCQPVNGKTSFTPSQFAQLTYVAGSDATPQNIVVVAQTGKALANGTLTQVTDSL
                     AVEIAASATGTRSINALNALVGTPAGVEGDITAIASEANIFTGFVGSGRPSLQTDGNF
                     TAVAGQLYRLDDLFHGQPSSGQTIAGYRIALSDAGIGGGQLLLNGQPVNGKTIFTADQ
                     FVHLTYQAGTDGTRQEMVAVAQTGTRLADGTLTHVTDSPAIQITATAAATGSINAMNA
                     MVNPPVGSDESTAVIASEANILTGLASSGRPTLQITLLSASPVAQADLPGLQGAYSTA
                     GAMQSESELDLSRYYPGATSGYESPGAFASVNTSAVAALLLLGGSATGAFQTADDLAA
                     TSQAIRAYTVVSGL"
BASE COUNT          705 a         1322 c         1047 g          730 t
ORIGIN      
        1 catccgtcag gcggatcgcc gcatccaccg cctcaatgcg ggctcgatcc gccgcgccga
       61 tgcgttgtat gctcactacg ttgacattgc cgcgcatggc cgcgcttgta tcatgaatcc
      121 gcaccagtct gaaggcagcc atggttgcgc gcattgtcct gctcaatggc gtcggcagtg
      181 ccggcaagac gtccatcgcg aaagcgctcc agagtatcgc acacgtccct cttcttcaca
      241 tcaagatgga cgcatttctc gacatgctgc ccgaagccat ggtcggacat cccgatgggc
      301 tggttttcga gcccatggaa ttggacggca aaccggcggt cgcgatcagg tccggcccgg
      361 ttgccgaccg tgctttccgc ggcatgcgtc actcggtcgc tgccctggcg gcacaaggca
      421 acgatctggt tgtcgatgag gtgctgctcg gtacgaaggc gcgcgaatac gcagaactgc
      481 tctcgccctt ccggctctac cgcgtgggtg tctttgctcc gctcaacgta cttgaggcac
      541 gcgaacgtca gcgtggcgat cggctgatcg gtttggcgcg ctggcagtac gaccgggtcc
      601 accagaacat gacatacgat cttgaactcg ataccagccg cgcatcaccc atgacatgcg
      661 cggaactgat cagggatacc ttcagcctgt agacaccgca cgacgcagcg cggcccgagc
      721 caggagccgc gtggaatcca gtgtcggcag cggcgaatta tcatcattga tgatcagcgg
      781 aatttccgtg cagcccaaca cgaccccatc gcaccccgcc tgcttcatcc ttgcaatcac
      841 ccgctgatgg aatgcaaccg cctccggccg gaacacgccg cataccagct catccatgat
      901 gatgcgattt agctccgccc gttcctccgt gtcaggtcgc atatactcag acggatacac
      961 cggtcccgtc accaaccacc gcgtccccag caatccgacc cgccggaacc cgcgcgtcgc
     1021 cgcctcttcc gccacagcag atgcgatatg cagccacggc agcggcgacc tctcaacgat
     1081 cagtgggagt gcctgatgga tcgtgttgtc cgggcagatc aggaaatccg ccccgatcgt
     1141 cgccaacttg cgcgccgagg ccagcatcag ctcgcccaca cccgcccaat catcgcgatc
     1201 cagccagtgc acatactccg ccagcgacgg cgtgtgcatg gagacttccg gatgcgagtg
     1261 tggtcccaac agccgcgcac cctcggcaca gatggtgcgg tagcagagcg acgcgccctc
     1321 agcagagcag gcgacgacgc cgatgtgctg cggcatgcgt atctcaccaa tatcttgcga
     1381 accacacctt cactaccagg gaacagtgct gccgagatag tccaggtatt gcagccctgc
     1441 cttcccttgc acggcaagca gcgtgcgcac aacatttgga atgctctctt ctatgccgag
     1501 acgcccgctc gatccaccca tatccgtgcg cacccagccc ggtgccatca ggactatcgc
     1561 ccgcttgtcc tcggcatgac gcgccgcaaa gcttcgcatg aacgtgttca gcgccgcctt
     1621 gcttccccga tacacctcgc gcatcccggt cgtattgttg gtaacgctac cctgcccgga
     1681 cgacatcacg ccgatcgtcc catccggaat taccaggtca tgcaaggcct ccaccacgcg
     1741 catcgggcta agcgcgttcg tcaccatcac gcgaacgaac tcttccgtgg tcacctcacc
     1801 gatagtctcg tccggattgt tggtcacacc cgcgttgacg aacagcatgt cgaagctgcg
     1861 ctccgccaaa cgcgagcgca atgcagcaat ctgctcgggc acagtgatgt ctatcgtctc
     1921 gatatcgaca cgtcctgtcg ctgcatcggc gagttcgtgg agcctggtgc gcgcctcgcc
     1981 acgcaccgtg cccaccacat tccacccatg cttcaggaac tcttccgcca tcgcgtggcc
     2041 gaggccgcgc gacgcgccaa tcagaagcag gctcttcgcc acgatcgcaa tccttctgct
     2101 gtccgcgctc acaatcggtg cgcatcaaat atcggactcc gccgtgtcga aaatcagctc
     2161 ttcgcgaata gccaggcgga accggccaag gccgtccgcg gggttgccat ttcggccagc
     2221 ttgcacctca ggctcacaac ccgctcacca ccgtgtaagc cctgatggcc tgagaggtcg
     2281 ccgcaagatc atcagccgtc tggaatgcac ctgtcgccga gccgccaagc agcaacagtg
     2341 ccgccaccgc cgacgtattc acgctggcga acgcgcctgg tgattcatat ccggatgtag
     2401 cgccaggata ataccgcgac agatcgagtt ccgattcaga ctgcatcgcg ccggccgtgc
     2461 tgtaggcgcc ctgcagccct ggcaggtcag cctgcgcgac cggcgaggcc gacagcagcg
     2521 ttatctgcaa cgttggccgc ccggagcttg ccagtccggt aaggatgttg gcctcgctgg
     2581 cgatcaccgc cgtactttca tccgaaccca ctggcggatt gaccatggcg ttcattgcat
     2641 tgatagagcc ggtcgctgcc gcagtcgccg tgatctgtat cgccgggcta tcggtcacat
     2701 gtgtcagcgt gccgtctgcc aacctcgttc ccgtctgcgc caccgccacc atttcctgcc
     2761 gcgttccatc ggtgcccgcc tgataagtca ggtgcacgaa ttgatctgcg gtgaaaatgg
     2821 tcttcccgtt caccggctgc ccatttagca gcaactgtcc gccaccaatg ccggcgtcgc
     2881 tcagagcaat ccgataaccg gcaatggttt gtccgctcga cggctgccca tggaacaggt
     2941 cgtcgagccg atacaactgg cccgcaaccg cggtgaaatt cccgtccgtc tgcagcgacg
     3001 gccgcccgga gccgacgaag ccggtaaaga tgttggcctc gctggcgatc gcagtaatgt
     3061 cgccctccac cccagccggc gtgccaacga gtgcgttcag cgcgttgatg gaccgcgtac
     3121 cggtagcgct tgccgctatt tcaaccgcca ggctgtccgt cacctgcgtc agcgtgccgt
     3181 tcgccagtgc tttgccggtc tgcgccacca ccacgatgtt ctgtggcgtc gcatcgctgc
     3241 cagcgacata ggtcagttgg gcgaactggc ttggcgtgaa gctggttttc ccgttcacag
     3301 gctgacaatt cagcagcaat tgcccgctcc cgttccccaa ggcgacgcga tagccggcaa
     3361 gcgtctgccc gctcagcgcg cttgcctgga acagatcgcc gatccggaac gcatcaccgg
     3421 ccaccgcggt gaagttgcca tcggtccgca cagtcggccg cccagtaccg aggccagtca
     3481 gaatgcctgc ctcactcgcg atgccggcta catcaccctc cgctcctgtc ggcttgatga
     3541 ccaacgctcc ggcagcattg atcgagccgg tctgcgctga attcgcagtg attgctacag
     3601 ctgggctgtc gatcacctgg gtcagtgtgc catttgccag caacttcccg gtctgcgcca
     3661 ccacaatcag gttctgctgg gtcccatccg ccccggcctg ataggtcaac ttcgcgaact
     3721 cggctgccgt gaagctggtg tccccgttca ccggctgacc gttcagcagc aattgtcctc
     3781 cgccggtgcc gccatcactc aacg
//
LOCUS       DATGEM010000369         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_511920, whole genome shotgun sequence.
ACCESSION   DATGEM010000369 DATGEM010000000
VERSION     DATGEM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511920"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="istA"
                     /locus_tag="VKQ27_11745"
     CDS             <1..1434
                     /gene="istA"
                     /locus_tag="VKQ27_11745"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HLY89647.1"
                     /translation="RVGTIARQLHVHHGTVARVLAQAGLPRIGPPAHRSQIESYLPFI
                     HLTLEKFPTLTASRLYAMVRERGYRGSGDHFRHLIACYRPRPAAEAYLRLCSLPGEQA
                     QVDWGHFGHLVIGRARRPLMAFVMVLSYSRQIFLRFFLDARMESFLRGHVGAFTAWNG
                     CPRVLLYDNLKSAVLERHGDAIRFHPTLIAFSGHYRYEPRPVAVARGNEKGRVERAVR
                     YVRDNFFAARAFADLDDLNVQAETWCRGMAADRRCPGQQGLSVREAFTAETPRLLALP
                     DNPYPLIERVTVKAGKTPYVRFDLNDYSIPHTKVQRLLTVLADPNEVRIVDGAEVLAC
                     HRRSYDKGAQIEDAAHVRVLVEHKRAARHHRGTDHLAKVAPESQTLLLRAAERGDNLG
                     AITAALLRLLERYGAAELDAAIREAIERGVPHPNAVRLALEHRRELRQEAPPVAVILP
                     EHVKARDGHVQPHRLEPYDQLKEQGDD"
     gene            1427..2179
                     /gene="istB"
                     /locus_tag="VKQ27_11750"
     CDS             1427..2179
                     /gene="istB"
                     /locus_tag="VKQ27_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HLY89648.1"
                     /translation="MTNLDALHMRAQALNLHGLRAHWEEAAAAGWLTTLIEWEEQERT
                     RRSLERRLRAAHIGRFKPLCDFDWTWPKRCDRAAFEALMALDFLKDATNAILVGPNGV
                     GKSTLARNIAHQALIHGHTVLFTSAGQLLGELAALDSDSALRRRLRHYAGPRLLVIDE
                     VGYLSYSNRHADLLFELVSRRYEHNSTIVTTNRPFAEWREVFPNAACVVSLVDRLVHN
                     AEVLAIEGQSYRLKEAQERADQRARQRRKAKS"
     gene            2148..2390
                     /locus_tag="VKQ27_11755"
     CDS             2148..2390
                     /locus_tag="VKQ27_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89649.1"
                     /translation="MRANAAKPSHEQSLERAAAHHPPQDSGLLDAGAGLAVVELLDDL
                     RDRIWTHYSVQLLDQYREQYGPADSAHINTTEDPSL"
BASE COUNT          468 a          947 c          759 g          467 t
ORIGIN      
        1 cgcgttggca cgatcgcccg ccagctgcat gtccaccacg gcaccgtggc gcgggtcttg
       61 gcccaagctg gcctgcccag gattggaccg ccggcacatc gctcgcagat cgaatcctat
      121 ctgccgttca tccatctgac cctagagaag ttcccgacgc tgaccgccag ccggctgtac
      181 gcgatggtac gcgaacgcgg ctaccgtggc agcggcgatc acttccgcca cctcatcgcc
      241 tgttaccgac cgcgccccgc ggcggaggcc tatctacgtc tgtgcagcct gcccggcgag
      301 caggcgcaag tcgattgggg gcattttggt catctcgtga tcggacgcgc acgccgtccc
      361 ttgatggcct tcgtcatggt gctgagctac tcgcgccaga ttttcctgcg cttcttcctc
      421 gatgcccgta tggagagctt cctgcgcggt cacgtcggcg ccttcacggc ctggaacgga
      481 tgtccgcgtg tcctgctcta cgacaatctc aagagcgcag tgctggagcg ccacggcgat
      541 gccatccgct ttcatcccac cctgatcgcg ttctccggac attatcgtta tgagccgcgt
      601 ccggtggcgg tggcacgcgg caacgagaag ggccgcgtcg agcgcgccgt ccgctatgtg
      661 cgcgacaact tcttcgcggc ccgcgctttc gccgatctcg acgacctcaa tgtccaggcg
      721 gagacttggt gccgcggcat ggcggcggat cgccgctgcc ccggacaaca gggccttagc
      781 gtccgcgagg ccttcaccgc agagacgccc aggctgctgg cgttgcccga caacccctat
      841 ccgctcatcg agcgcgtgac cgtgaaggcc ggcaagaccc cctacgtgcg cttcgacctc
      901 aatgattatt ccattccgca caccaaggtg cagcgtctgc tcacggtgct ggccgatccg
      961 aacgaggtgc gcatcgtcga tggcgcggag gtgctcgcct gccatcgccg cagctacgac
     1021 aagggggcgc aaatcgagga tgccgcccat gtccgggtct tggttgagca caaacgcgcc
     1081 gcgcgtcacc accgcggcac cgaccatctc gcaaaggtgg cgcccgaaag ccagaccttg
     1141 ctcctgcgcg ccgccgagcg cggtgacaat ctcggagcca tcaccgccgc cctgctgcgg
     1201 ctgctggagc gctacggcgc cgccgagctt gacgccgcca tccgcgaggc gatcgagcgc
     1261 ggcgtgcctc atccaaatgc ggtgcggctc gcactcgagc accgccgcga actgcgtcag
     1321 gaggcgccgc ccgtcgccgt cattctgccc gagcatgtca aggcgcgcga tggccacgtg
     1381 cagccgcacc ggctcgaacc ctatgaccaa ctcaaggagc aaggcgatga ctaacctcga
     1441 cgctctgcac atgcgcgccc aggcgctcaa cctgcatggc ctgcgggcgc attgggagga
     1501 agcggccgcg gccggttggc ttacaacgct gatcgagtgg gaggaacagg agcgcacgcg
     1561 ccgaagcctg gagcgccggc tgcgggccgc tcatatcgga cgcttcaaac cgctctgcga
     1621 cttcgattgg acatggccca aacgctgcga ccgcgccgcc tttgaagcgc tgatggcgct
     1681 cgacttcctc aaggacgcaa ccaacgccat cctggtcggc cccaacggtg tcggcaaatc
     1741 gacgctcgca cgcaacatcg cccatcaggc cctcatccac ggtcacaccg tgctgttcac
     1801 cagcgccggc caattgctcg gcgaactcgc cgcactcgac agcgattccg ccctgcgccg
     1861 gcgcttgcgt cactatgccg gccctcgtct tctggtgatc gacgaagtcg gatatctctc
     1921 ctattccaat cgccatgccg acctgctgtt cgagctcgtc agccgccgtt acgagcacaa
     1981 cagcacgatc gtaaccacca accggccctt cgccgaatgg cgcgaggtct ttccaaacgc
     2041 cgcctgcgtc gtctccctgg tcgatcgcct ggtccacaac gccgaggtcc tcgccatcga
     2101 gggccaatcc tatcgcctca aggaagccca ggaacgagcc gaccagcgtg cgcgccaacg
     2161 ccgcaaagcc aagtcatgag caaagtctcg aacgcgctgc cgctcaccat ccccctcaag
     2221 attccggcct actggacgcc ggagcaggcc tcgccgttgt cgaactgctt gatgacctgc
     2281 gcgatcgcat ctggacgcac tacagcgtgc agttgcttga tcaataccgc gagcaatacg
     2341 gccccgccga cagtgctcac atcaatacaa ctgaagatcc atcgctctga gtcgccatgc
     2401 cgccaaaact tcaggggcct cgacggcccc tttatcttca ccagcgcgag cttcgccgtc
     2461 tatgcgcgga ttcacaccgc cggtaacagt gatctggacg acacgcgcgc catatacgat
     2521 agcctcacgc gttccagtac cgctgcagca acttcacgag cgagaattcg cacacgagct
     2581 gccacagttc ctgtcttcgt ttcttggtgg ccatagcgct ctccattccg gtctgttctc
     2641 g
//
LOCUS       DATGEM010000370         6062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_765349, whole genome shotgun sequence.
ACCESSION   DATGEM010000370 DATGEM010000000
VERSION     DATGEM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765349"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VKQ27_11760"
     CDS             complement(<1..453)
                     /locus_tag="VKQ27_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89650.1"
                     /translation="MRRVAKWLGWIICIIVGAPVLLVALALIAANTGPGRDAIESLVP
                     KLTGDTVRLAGLAGRFPDAVRARRIELRDASGDYATIENATFDWSPLQLLHWQVVIDR
                     LAADAIDVLRMPASSSSSSGSTGLPAPVVLRELTVGRIDIGAAVAGTAA"
     gene            complement(453..2267)
                     /locus_tag="VKQ27_11765"
     CDS             complement(453..2267)
                     /locus_tag="VKQ27_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HLY89651.1"
                     /translation="MRHILFFLLVLLTWTGEALGADPQPYAVTLKPTGNTALDQALHD
                     ASQLVSLRENAPVGPFALVARAREDAGRFQTALNSFGYYKAIAVLTIDGHALDDATLP
                     DTLDHAPANPPVSVVVSFDLGPLFHLGAVTIQGNIPEAARAALGLAQGAPAVASGVLA
                     AQQRLLSAIRAAGHPLAKVDLPPVTLRPAANLMDVTFQASAGPYASIGTISVSGLKTV
                     NESYVRRRLLLHSGEKFSPEAIEAARADLAGIGVFSVVRISPADHLAPDGTIPIQIDV
                     TERPLHAVELGIAYSTDLGLNPTAAWHDRNLFGNGEQLNITAGTELGGNAVTKPGYNA
                     GIQFIKPDFLARNQSLEIDLDAIKQSLEAYDQRALMEKIEMARKLFEHWTIGYGLSGE
                     QEEITQEGVRANYNLIGIPLSAKFDNTDNLFNPTRGFRAALLLTPTESLGHVNATFMI
                     SQITGSAYFDVSGGGRTVLATRGLIGKAFGAQQFSLPPDQRFYAGGSSTVRGFRYQSI
                     GPQFPDGNPQGGTGISAGSIELRQRILGNWGAVGFVDAGQVTANGAPFTSNWRIGAGV
                     GARYYTSIGPIRLDVAVPLNREPHGDAFELYIGIGQAF"
     gene            2404..3288
                     /locus_tag="VKQ27_11770"
     CDS             2404..3288
                     /locus_tag="VKQ27_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLY89652.1"
                     /translation="MLAGQDVAHNPLFAERKLGLLRPFRVVRRAGGIVLLTLPCMLVQ
                     AVFIVLPGRLKVTFARFYWSTACRLLGVGVRVIGAPASAAGCPVIFVSNHSSWLDVPV
                     LGGRIDACFVSKDEVGQWPVVGTVARLGRTIFVSRQRQATGRERDIMRERLAAGDNLL
                     LFPEGTTSDGSRVLPFRSSFFAIAEGPEAPLIQPISVVYDRLSGLPTGRACRPVFAWY
                     GNMDLASHFWRFAQYKGLRVTVLLHAPIDPTRFASRKALSHAVWQTVADGASRLRQNR
                     PVAAKDPIALGGTEPAYA"
     gene            3420..4748
                     /gene="miaB"
                     /locus_tag="VKQ27_11775"
     CDS             3420..4748
                     /gene="miaB"
                     /locus_tag="VKQ27_11775"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLY89653.1"
                     /translation="MKRLHVITWGCQMNMYDSGRMADVLAPLGYVPTPMPDGADMVIL
                     NTCHIRDKAAEKVFSELGRLRRLQEQRAADGGRMILAVAGCVAQAEGEQILARAPYVD
                     IVLGPQTYHRLPEMVARAALAGGAVIETDFPVEDKFDFLPDAAPQGITAFLTIQEGCD
                     KFCSFCVVPYTRGAEQSRPATAVIAEACRLVAQGTREITLLGQNVNAWHGEAGGLGRL
                     LHDLAELPGLLRLRYTTSHPRDMDDDLIAAHRDLPSLMPFLHLPVQSGSDRMLKAMNR
                     GHTAADYLGTVEKLRSARPDIALSSDFIVGHPGESEADFQATLALVREVRFAQAFSFK
                     YSPRPGTPAAVTPDQIPEPEKERRLTELQALLRDQQARFNAGCVGLRLPVLFTGPGRH
                     PGQIAGRSPFLQPVHITGSDNLIGNEFPVQIAAALPNSLSATLVEERVCA"
     gene            4838..5794
                     /locus_tag="VKQ27_11780"
     CDS             4838..5794
                     /locus_tag="VKQ27_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012552948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLY89654.1"
                     /translation="MLLGDHDRHMVRIEQALGVRLSCRGNRVAISGEASRVDAAQAAL
                     EGLWRRLERGESIGRSEVEAAIRMADDADPRLPLSDLPAIRTRRGAVGPRSPGQAAYI
                     EALASHDMVFGIGPAGTGKTYLAVAQAVAMLQAGKVERIVLSRPAVEAGERLGFLPGD
                     LKEKVDPYLRPLYDALHDMMPGDQVVRRLANGEIEVAPLAFMRGRTLAHCYAILDEAQ
                     NTTPVQMKMWLTRMGEGTRMVVTGDLSQVDLPAGTRSGLRDALDTLEGIAGIGVARMG
                     TRDVVRHPLVARIVDAYDQRAAAEGDKRREARRRGETDGTGK"
     gene            5798..>6062
                     /locus_tag="VKQ27_11785"
     CDS             5798..>6062
                     /locus_tag="VKQ27_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation factor"
                     /protein_id="HLY89655.1"
                     /translation="MDPGGSSGPAQAGFQDEVNVIVAEPAWRRLIPHAEFVAARAARA
                     AGASGTVVLAADRTVRRLNARHRRQNKSTNVLTYTAPAPEMLLA"
BASE COUNT         1064 a         1998 c         1941 g         1059 t
ORIGIN      
        1 tgccgccgtg ccggccacag ccgcgccaat gtcgatacgc cccactgtaa gctcgcgcag
       61 cacgaccggc gccggcaatc cggtacttcc ggacgaagac gatgagctgg ccggcatacg
      121 caacacgtcg atcgcgtcag cagccagccg gtcgatcacg acttgccaat ggagcagctg
      181 caacggtgac cagtcgaatg tcgcattctc gatcgtggcg taatcgccag aggcatcgcg
      241 cagttcgatc cgcctcgcgc gcacagcatc gggaaagcgg cctgccagcc cggccagacg
      301 caccgtatcg ccggtcagtt tcggcaccag ggactcgatc gcgtcgcggc caggaccggt
      361 gttggcggca attagcgcga gcgcgaccag cagtaccgga gcgcccacga tgatgcaaat
      421 gatccatccg agccacttcg cgacgcgccg catcagaacg cctgcccaat gccgatatac
      481 agctcgaaag cgtcgccgtg aggctctcgg ttcaaaggaa ctgcgacatc gagacgaatt
      541 gggccaatcg atgtgtaata gcgcgctccc acccccgccc cgatacgcca gttgctggta
      601 aagggggctc cgttggcagt gacctggccg gcatcgacga agccgacagc accccagttc
      661 cccaggatgc gctggcggag ctcaattgag ccggcactga tcccggtgcc accctgcgga
      721 ttgccatcgg ggaattgtgg cccgattgac tgatagcgga agccacgcac cgtgctggat
      781 ccgccggcgt agaagcgttg atccggcggc aggctgaact gttgcgcgcc gaacgccttg
      841 ccaatcaggc cgcgcgtcgc gagcacggtg cggcccccac cgctcacgtc gaaataggcc
      901 gaacctgtga tctggctgat catgaatgta gcgttgacat gcccgagcga ttccgtcggc
      961 gtcagcagca gcgccgcgcg gaatccgcgc gttggattga acagattgtc cgtgttgtcg
     1021 aatttggccg acagcggaat gccgatcagg ttgtaattcg ctcggacgcc ttcctgggtg
     1081 atttcctcct gctcaccact tagcccgtag ccaatggtcc agtgctcgaa gagcttacgg
     1141 gccatttcga tcttctccat cagcgcacgc tgatcgtacg cctcgagact ttgcttgatc
     1201 gcgtcgagat cgatctccag cgactggtta cgcgccagga aatccggttt gatgaactgg
     1261 atgccggcat tgtatccggg cttggtcacc gcattgccgc caagctccgt gccagcggtg
     1321 atgttgagtt gctcgccgtt gccaaacagg ttgcggtcgt gccaggcggc tgtcgggttg
     1381 aggcccaggt cggtcgaata ggcaatgcca agctccaccg cgtgcagcgg tcgttcggtg
     1441 acgtcgatct ggattgggat cgtgccatca ggcgccagat ggtctgcggg cgaaatgcgc
     1501 acgacggaga aaacaccgat ccctgccaga tcggcacggg ccgcctcgat cgcctccggg
     1561 ctgaactttt cacccgaatg cagcagcagc cgacggcgca catagctttc attcaccgtc
     1621 ttcagcccgc tgacgctgat cgtgccgata ctggcgtagg gaccggcgct ggcctggaac
     1681 gtcacgtcca tcaggttggc agccggacgc agcgtgaccg gcggcaaatc taccttcgcc
     1741 aacgggtgcc cggctgcacg aattgcgctc aacagtcgct gctgcgctgc cagcacgccc
     1801 gacgccacgg ctggcgcccc ctgcgcgagt cccagtgcgg cacgcgccgc ttcggggata
     1861 tttccctgga tggtgactgc gccgaggtgg aaaagtggcc caagatcaaa actgaccacc
     1921 acggagaccg gaggattggc cggcgcatga tccaatgtat ccggcagcgt ggcgtcatcc
     1981 agcgcatggc cgtcgatcgt aaggacggcg atggccttgt agtagccaaa gctgttcaat
     2041 gcggtctgaa accgccccgc gtcttcccgc gcccgagcca ccagtgcaaa cgggcccacc
     2101 ggcgcattct cgcgcagcga cacaagctga gaggcatcgt gaagggcctg gtccagggcc
     2161 gtgttgcccg tcggcttcaa tgtcaccgca taaggctgcg gatcggcgcc caatgcctca
     2221 cccgtccagg tcaacagcac gaggagaaag aacagaatat ggcgcattag cggcacggta
     2281 gccggtgcag tgttccccca agtgtcacgg ttgcgccatt gttaggaaac tgtgagcgag
     2341 ctatccggat ccatcatgag ggaatcctcc cgtcgtccgc gtcgcctgaa agcaggccgc
     2401 ccggtgctgg ccggccagga tgtcgcccat aatcctctgt tcgccgagcg caaactcggc
     2461 ctcttgcggc cgttccgcgt ggtccgcaga gccggcggca tcgtgctgtt gacacttcca
     2521 tgcatgctgg tccaggcagt ctttatcgtg cttcccggcc gcctgaaagt gacgtttgca
     2581 cggttctatt ggtctacggc gtgccgcctg cttggcgtcg gcgtacgggt gattggggcg
     2641 cccgccagcg ccgccggctg cccggtgatc ttcgtctcca atcattcgtc ctggctggac
     2701 gtccccgtac tcggggggcg aatcgacgcg tgcttcgtgt cgaaggacga ggtgggacaa
     2761 tggccagtcg tgggcaccgt agcgcggctg ggccgcacga tcttcgtgtc gcgccagcgc
     2821 caggcgaccg ggcgcgaacg cgacatcatg cgcgagaggc tggcagccgg cgacaacctg
     2881 ctgctgttcc ccgaaggcac cacctcggac ggctcccgcg tgctgccgtt ccgcagttcg
     2941 tttttcgcca tcgccgaagg cccagaagcg ccactgatcc agcccatttc ggtggtctat
     3001 gaccggctta gcggtctgcc aacagggcgg gcgtgccggc cagtgtttgc ctggtacggc
     3061 aacatggacc tggcgtcgca tttctggcgg tttgcgcaat acaaggggct gcgggtcacg
     3121 gtgctgctgc acgcgccgat cgatccgacg cgcttcgcca gtcggaaggc gctgtcgcac
     3181 gcagtctggc agacggtcgc cgacggggca tcgcgattgc gacagaaccg gccggtggca
     3241 gcaaaggacc cgatcgcgct tggggggacg gaaccggcct atgcgtgagt gaacttgtgg
     3301 ttgacaggac gacgcccatt cctgcctgat tgctcccatg caccgaagcg gttccgcttg
     3361 acccgcctgg caggtcccac gataccaccc gccctttcct ggcaggagta tcgcgattga
     3421 tgaagcggct gcacgtcatc acctggggct gccagatgaa catgtatgac agcgggcgga
     3481 tggcggatgt gctggccccg ctaggctacg tgccgacgcc gatgccggac ggcgcggaca
     3541 tggtgatcct caacacctgc catatccgcg acaaggcggc ggaaaaggtg ttctccgaac
     3601 tcggccgcct gcgccgcctg caggagcagc gcgcggctga cggcgggcgc atgatcctgg
     3661 cagtcgccgg ttgcgtcgcc caggcggaag gtgaacagat tctggcccgc gcaccgtacg
     3721 tggacatcgt gctcggcccg cagacctatc accggttgcc cgagatggtc gcgcgcgcgg
     3781 cccttgccgg cggggcagta atcgagacgg acttccccgt ggaggacaag ttcgacttcc
     3841 tgcccgacgc tgcgccgcag ggtatcaccg cattcctgac catccaggaa gggtgcgaca
     3901 aattctgctc attctgcgtg gtgccataca cccgtggcgc cgagcagagc cgccctgcca
     3961 cggccgtcat cgccgaggcc tgccggctgg tcgctcaggg tacgcgcgag atcaccctgc
     4021 tcgggcagaa cgttaatgca tggcatggtg aagcaggcgg cctcggtcgc ctgcttcacg
     4081 acctggccga actgcccgga ctgctacgac tgcgctacac cacgtcgcat ccgcgagaca
     4141 tggacgacga cctgatcgcc gcccatcgcg acctgcccag cctgatgccg ttcttgcacc
     4201 tgccggtgca gtcaggctcc gaccgcatgc tgaaggcgat gaaccgcggc cataccgccg
     4261 cggattacct gggcacggtg gagaagctgc gctcggcccg ccctgacatc gcgctatcgt
     4321 ccgacttcat cgtcggccat cccggcgagt cggaggctga cttccaggcc acgctggcgc
     4381 tggtgcgcga ggtccgcttc gcccaggcat tcagctttaa gtactctccg cgccccggga
     4441 cgccggccgc agtgactccc gaccagattc cggagccgga aaaggagcgc cgcctaaccg
     4501 aactgcaggc tttgctgcgc gaccagcagg cacgattcaa tgccggctgc gtcgggctgc
     4561 gcttgccggt tctgttcacc ggcccaggcc gccatcccgg ccagatcgcc ggccgttcac
     4621 cgttccttca acccgtgcac attacagggt cggacaatct gatcgggaac gaatttcccg
     4681 tgcagatcgc cgctgccctt cctaactctc tgtctgcaac cctcgtcgag gagagagtct
     4741 gcgcttgagc cagaccacga tcatccctgc catcactgtc ccatccccgc acggcaaggc
     4801 cgtgacgctc cagttcagtg acaacacgct gttgccgatg ctgctgggtg accatgaccg
     4861 ccacatggtg cgcatcgagc aggcgcttgg cgtgcggcta tcctgtcggg gcaatcgcgt
     4921 cgcgatcagc ggcgaggcat cgcgcgtgga tgccgcccag gcggcactgg aaggattgtg
     4981 gcgacgactg gaacgtggcg agagcatcgg ccggtcggaa gtggaagcgg cgatccgcat
     5041 ggcggatgac gccgacccgc gccttcccct gtccgacctg cccgcgatcc gcacccgccg
     5101 cggcgcggtt ggtccgcgta gcccggggca ggccgcctat atcgaggcgc tcgcgtccca
     5161 cgatatggtt ttcggcatcg gccctgccgg aactggcaaa acctacctcg cggtggccca
     5221 ggccgtggcg atgcttcagg ccggcaaggt ggaacgcatc gtgctgtccc gcccggcggt
     5281 ggaagccggc gaacggcttg gtttccttcc tggcgacctc aaggaaaagg tcgaccccta
     5341 tttgcgcccc ctctacgacg cgctgcacga catgatgcca ggcgatcagg tcgtccgccg
     5401 cctcgccaac ggcgagatcg aggttgcccc gctcgctttc atgcgtggcc gcaccctggc
     5461 gcactgctac gccatcctgg acgaggccca gaatactacc ccggtgcaga tgaaaatgtg
     5521 gctcacccgc atgggcgagg gcacccgcat ggtagtcacc ggcgacctgt cgcaggtcga
     5581 cctgcccgcc ggcacccgct caggtctgcg cgatgcgctg gacacactgg aaggcattgc
     5641 tggcataggt gttgcgcgca tgggcacacg ggacgtggtt cggcatcctc tggttgcacg
     5701 catcgtagat gcgtatgatc agcgtgctgc ggcggaaggc gacaagcgac gcgaagcgcg
     5761 ccggcgcggt gagactgacg ggactggcaa ataggcgatg gaccccggag gaagtagtgg
     5821 ccccgcgcag gcggggttcc aagacgaggt caacgtcatt gtggccgagc cggcatggcg
     5881 tcggctgatt ccgcacgccg aatttgtggc agcacgcgcg gcgcgggcgg cgggcgcaag
     5941 cggcacagta gttttggcgg ctgaccgcac ggttcgccgc ctgaacgcgc gccaccggcg
     6001 acagaacaaa tccaccaatg tgctgaccta caccgctccg gcgcccgaga tgctgctcgc
     6061 gc
//
LOCUS       DATGEM010000371         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1313331, whole genome shotgun sequence.
ACCESSION   DATGEM010000371 DATGEM010000000
VERSION     DATGEM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313331"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VKQ27_11790"
     CDS             complement(<1..776)
                     /locus_tag="VKQ27_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HLY89656.1"
                     /translation="MADGSIDTRTAHISAEEALALHATGRPGKLETRISKPITTARDL
                     SLAYSPGVAEPCLHIQRDASLAYDYTAKGNMVAVVSNGTAVLGLGNLGAVAAKPVMEG
                     KIALFKRFADIDGIDLEIDSENVDDVVNAVRFLAGFGGINLEDIKAPECFVIEQRLRE
                     LMDIPVFHDDQHGTAIVAAAGLINACHLTGRELRDTRLVVNGAGAAGIACVELLKAMG
                     MRPEHVVLCDTKGVVYQGRTEGMNQWKSAHAAATKARTLT"
     gene            1394..2638
                     /locus_tag="VKQ27_11795"
     CDS             1394..2638
                     /locus_tag="VKQ27_11795"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLY89657.1"
                     /translation="MPAPQAEPDTGIQTLLDRAPAVAVAGEQLNAALLRRFYARHDFE
                     PVWTTRQAQANSLVDAVLRAGDQGLDPELFHASLLRHRAMLRPLDRDLLLSDAFLSYA
                     DALARGAVPVERRRDDEVLTPGQIDVAAALDAAIGSPDPGAVIEGLAPTTPTYRALRL
                     ALQKVRPGSRGAMDRLRTIEVNLERQRWLPRPLPEDRAWVNVADERLVLYRGGQAVFS
                     TKVIVGEDTRHNQSPEFRTTIDASLFNPPWVIPSDIVTNEILPQISRDPDYLKRNNMI
                     LLAGGEAEQLPGPDAGLGLIMFDMPNRFDVYLHDTPDKRLFKRDNRRISHGCIRVQEP
                     REFAALLMGEPIDMIDQGIALGGTKREKLPTPVPVFVVYQTAFVDAGGTLQFRRDFYN
                     RDGEIWQQLQNQPHGTYASALK"
     gene            2861..3280
                     /locus_tag="VKQ27_11800"
     CDS             2861..3280
                     /locus_tag="VKQ27_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89658.1"
                     /translation="MTKYLHTMIRVSSLEDTIAFFGVLGLREIRRRDDEKGRYTNVFL
                     AAPGDDAAPVELTYNWDSEPYTGGRNFGHLAYAVDNIYDTCNAFIARGVTINRPPRDG
                     RMAFIRTPDGISIELLQAGNALPPAEPWSSMSNTGSW"
     gene            3652..>4273
                     /locus_tag="VKQ27_11805"
     CDS             3652..>4273
                     /locus_tag="VKQ27_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLY89659.1"
                     /translation="MAPHPQRLTVTRRAWPLARPLKTAHGVETTVDAVVAEISDVESR
                     GRGECMPLRRYGESADSVVAALEAMKGAITSGLARDTLQSALPPGATRNALDCAFWDM
                     DAKRAYCSVAELAGLSAVTPVVTAFTLDFDTPERMAEQAAANRTRPLLRLECFGDGDI
                     ERVRAVREAVPRARLIIDANESWNERQLGEYMPTLSDLRVELIEQPL"
BASE COUNT          755 a         1268 c         1494 g          756 t
ORIGIN      
        1 tcggtcagcg tacgggcctt ggtggcggcg gcgtgggccg acttccactg gttcatgccc
       61 tcggtgcggc cctggtaaac gacgcccttg gtgtcgcaca ggacgacatg ctcggggcgc
      121 atgcccatcg ccttcagcaa ttcgacgcag gcgatgccgg cggcgcctgc accgttcacc
      181 accagccggg tgtcgcgcag ttcgcggccg gtgaggtggc aggcgttgat caggccggcg
      241 gcggcgacga tggcggtgcc gtgctggtca tcgtggaaca ccgggatgtc catcagctcg
      301 cgcaggcgct gctcgatgac gaagcattcc ggggccttga tgtcctccag gttgatgccg
      361 ccgaagccgg cgaggaagcg gacggcgttc accacgtcat cgacgttctc ggagtcgatt
      421 tcgaggtcga tgccgtcgat atcggcgaag cgcttgaaca gcgcgatctt gccttccatc
      481 accggcttgg cggcgacggc gcccaggttg ccgaggccga ggacggcggt tccgttggaa
      541 accacggcga ccatgttgcc cttggcggtg tagtcgtagg cgagggaggc gtcgcgctgg
      601 atgtgcaggc atggttcggc gacgccgggc gaataggcga gcgagaggtc gcgcgcggtt
      661 gtaatcggct tggagatgcg cgtttccagc ttgccggggc ggccggtagc gtggagggcg
      721 agcgcttcct cggccgaaat gtgggcggtg cgggtgtcga tgctgccgtc ggccattgga
      781 gtttcccctg cgctacgcgt cgttcagggc atcatgggga gaaggggcgc ggaagggaag
      841 ccgggggcgg ctcaggcgca aggaagcagc tgatgctcgc gcgggttggc gcgcggtcga
      901 gcgaacgtag cgacccaatc ccgcacgaga ttgcatcgct acgctcgcaa tgacagcgtc
      961 cggctggact tgagtggtgt tagcctcgag ctggcggagc cggcggcgga ggtggcggcg
     1021 gcggtggggc tgcctggaca ggcgcgggcg gcggtggggg cgctggctgg gaacaggccg
     1081 cgagaagcgc aatgggaacc aggacggcaa ccattgctgc gccacggaag agtttactgc
     1141 gggccattcc tcactccttt gttgcactat gcgtagaaac agttaatttt aagggcaatt
     1201 ctcaacgaag agtcaaactg ctggttgcgt agccgacaaa ggtcgacgaa tggcacgaat
     1261 gtgtgcgccg cggcgggagg ccggatctgg ccgccaaaag gtttcgactt ccgcctgggt
     1321 tcgggcgttc gttcttgcca tggtatgcgc actggctgcg tgcggcagag atccgcagcc
     1381 tgacgcagag gttgtgcccg cgccgcaggc cgagcctgac actgggatcc agacgctgct
     1441 ggaccgtgct cctgcagtgg cggtggccgg tgagcagctg aacgccgcgt tgctgcggcg
     1501 cttctatgcg cgacatgatt tcgagccggt ctggaccacg aggcaagcgc aggcgaattc
     1561 attggtcgat gccgtgctgc gcgccggcga ccagggcctg gatcctgaac tgtttcacgc
     1621 cagcctgttg cggcacagag cgatgttgcg gccgctcgac cgcgacctgc tgctgtcgga
     1681 cgccttcctg tcgtatgccg acgcactggc ccgcggcgcc gtgccggttg agcgccggag
     1741 ggacgacgag gtcctgactc ccgggcagat cgacgttgcg gctgcgctcg atgccgcaat
     1801 cggcagccct gatccggggg cggtgatcga ggggctggca ccgacgacgc cgacctaccg
     1861 ggcgctgcgc ctggccttgc agaaggttcg cccggggagc aggggcgcga tggaccgcct
     1921 gcgcacgatc gaggttaacc tggaacgcca gcgctggctg ccgcgtccgc tgccggagga
     1981 ccgggcctgg gtgaacgtcg ccgacgagcg gctggtcctc taccgcggcg ggcaggcggt
     2041 tttttcgacg aaggtcatcg tcggcgaaga taccaggcac aatcagagcc cggaatttcg
     2101 taccacgatc gacgccagcc tgttcaaccc gccatgggtc atcccgagtg atatcgttac
     2161 caacgagatc ctgccgcaaa tcagccgcga cccggattac ctgaaacgga acaacatgat
     2221 cctgctggcg ggcggcgagg cggaacagct gccgggaccc gatgcggggc ttggactgat
     2281 catgttcgac atgccgaacc ggttcgacgt ttacctgcac gataccccgg acaagcgcct
     2341 gttcaagcgc gataaccgcc gcatcagcca cgggtgcatc cgggtacagg agccgcgtga
     2401 atttgccgcg ctgctgatgg gggaacccat cgacatgatc gatcaaggga tcgcgttggg
     2461 cggcaccaaa cgcgagaaac tgccgacgcc ggtgccggtg ttcgtggtgt accagacggc
     2521 gttcgtcgat gccgggggga cgctgcaatt ccgccgcgac ttctacaacc gcgacgggga
     2581 gatctggcag cagctgcaga accagcccca cgggacctat gcctcggcgc tgaaatagcg
     2641 cctggagccg ttggggtagc gacgaagcag ggcggacggt ggcggcaggc tccatgacaa
     2701 tatatctaac ttcatgttag agacgtgtcc aggaaaatct tcttgtaggc tgcatccata
     2761 atgacgctcg gcgatggtgc gcggaatcgg cattgtgctt ccaagccgga catttcgcac
     2821 tatggtgtcg cccggctttg acaaccgcgg aggacaggcc atgaccaagt atctgcacac
     2881 gatgatccgg gtcagttcac tggaggatac catcgccttc ttcggggtac tcgggctgcg
     2941 cgagatccgc cgccgtgacg acgagaaggg ccgctacacc aacgtgttcc tcgccgcacc
     3001 gggcgatgac gcggctcccg tggagctgac gtataactgg gattccgagc catataccgg
     3061 cggccgcaac ttcgggcatc tcgcctatgc ggtcgacaac atctacgaca cctgcaatgc
     3121 gttcatcgcg cgcggggtaa cgatcaaccg cccgccgcgc gacggccgca tggcgttcat
     3181 ccggacgccg gacggcatct cgatcgaact gctgcaggct ggcaacgcgc tgccgccggc
     3241 tgaaccttgg tccagcatgt ccaataccgg gtcctggtag ggcgggtggt ttcgtgtgaa
     3301 atcaggcgcc gagcgcgaca ccttcgtgac cggcggcaat gacgtcattc tcagcatcgg
     3361 gatcacgtcc gcgttgcagt gcagcgattg acagggtttg gcgcctggct ctgaggttga
     3421 tcacccgtgc ggatcgcaga attcaacgca aagttcgcta aggaaagcga aggccaccaa
     3481 gaagatcctg tattgaccga aagcacttcg cgatcttcgc gcgcccttcg cgggctttgc
     3541 gttgaatcat cctcccgggt gctggtcatg acggaggatg aatggtcagt acccgcggcc
     3601 gctggtatcc gacggcatgc gtagaagatc gccgaaaggg ttgggataga gatggcgcca
     3661 catcctcaac gactgacagt cacccgccgc gcgtggccgc ttgcgcgccc gttgaagacc
     3721 gcgcacgggg tcgagaccac ggttgacgcc gtcgtcgccg aaatctccga cgtcgagtca
     3781 cgcggccgcg gcgaatgtat gccgctacga cggtacggcg agagcgccga cagtgtcgtc
     3841 gccgcgcttg aagcgatgaa aggcgcgatc acctcggggc tcgcgcgcga cacgctgcaa
     3901 agtgcgctgc cgccgggtgc tacgcgcaac gcgctcgatt gcgcattctg ggacatggat
     3961 gcgaagcgcg cttattgcag cgttgcggaa ctggctgggt tgagcgctgt gacgccggtc
     4021 gtgaccgcct tcacgctcga cttcgacacg ccggagagga tggctgagca ggcggccgcc
     4081 aatcgcaccc ggccgttgct gcgattggaa tgttttggcg atggcgatat cgagcgggtg
     4141 cgcgcggtgc gggaggcggt gccgagggcg cgccttatca tcgatgccaa tgagagctgg
     4201 aatgagcgcc agcttggcga atacatgccg acgctgagtg atctgcgggt ggagttgata
     4261 gagcagccgc tgc
//
LOCUS       DATGEM010000372         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1419661, whole genome shotgun sequence.
ACCESSION   DATGEM010000372 DATGEM010000000
VERSION     DATGEM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419661"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1184)
                     /locus_tag="VKQ27_11810"
     CDS             complement(120..1184)
                     /locus_tag="VKQ27_11810"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89660.1"
                     /translation="MLAGKTEAKLEGEDGMAPYREVITAPAAWTNRSIGGKAGLTQRL
                     TAAQLAAFDAALARTRHLQPQETTREDFAHPEISALVAGLDETIRRGRGAVLLSGLTR
                     ATHSEEDMERIYWGIGVRLGDPAVQSMLGDRLGHVQHVKDDPVARGYRSNEELTPHTD
                     SYRVVGLMCLQRAETGGLSRIVSSLAIHNEILATRPDLLEPLYEGYHYAVAEAQFSSK
                     PVTDFKIPVFCNVDGVVSCNFVRDFMRRAAKLRGETFPKKLAEAIEYMSEVAERDDIG
                     IQFLLEPGEMLLWHNFQMLHAREAYRDSPEHTRHLLRLWLKIDNDRPICDEILARAKI
                     YERVHQERSGRVPESLVAAG"
     gene            1224..1967
                     /locus_tag="VKQ27_11815"
     CDS             1224..1967
                     /locus_tag="VKQ27_11815"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY89661.1"
                     /translation="MRLAGKVAIISGAASGMGAATARLFAREGAKVVIADVLEHEGGQ
                     IAGSIGSAARFEHLDVTSEDDWAAVVSAATTHFGKLDILVNNAGISGSAEQDFYSTEA
                     WHRIMGVNATGVFFGIKHAVQAMKAGGGGAIVNLSSIAGIIGSEHVHMAYNASKAAVR
                     LMTKSVAVQHAKDNIRANSVHPGIMPAMRTSGRTADPAIRAERMRVIPMRRPGEVDEV
                     AYAILFLASDEASYITGSEIHVDGGAIAI"
     gene            complement(1986..2504)
                     /locus_tag="VKQ27_11820"
     CDS             complement(1986..2504)
                     /locus_tag="VKQ27_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89662.1"
                     /translation="MAYAGTASTRPHLVPQVAVPRFRWPHLSRRAHALLMIGLMISPA
                     FLADDIGYCVQRLFMTADQIAMQQESDQRMPADIYVFHVACSDPGASTLQRERWSEFA
                     ANHGWPRYPQAGPGCFRPDRALYGVAGLKMFNVACPTMVLSVADQHRWVAFAANRGWT
                     EYAQAGVGCVDP"
     gene            2710..>3716
                     /locus_tag="VKQ27_11825"
     CDS             2710..>3716
                     /locus_tag="VKQ27_11825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HLY89663.1"
                     /translation="MTSRHGQRMETDVADLRISEVADEIIEIQHAAVLEGDFEIDLGT
                     TFTDPKARAEFSRCPTLEGLRHYRLRDVTLDASTMLLVRDRQRIAETRYLVPDSAYAD
                     MLTKPLHPMPLDPAEHYIIGGNRAWHNYFHWMVQAVPAIDIGLRHANHRRVTIVLPPD
                     PRPWQEETLALLGYQEVPRYILHGSGHFLLQSAEFSELVGARGPELVARVATATYKRM
                     SQAVPWTEPGAEEIYVARTDSQNRIAENEAELMEMLQRQGVSIVVPGSLTVSEQIATF
                     RAARLVIGPHGAGMSNIAFCRPGSFVYEMLPRHYPNFAFNRIAQASGLNYVADMFESF
                     GS"
BASE COUNT          704 a         1214 c         1124 g          674 t
ORIGIN      
        1 aagaccggag cggttggacg cgtcaacgca aattatcacg tgtagtgatc tccggctgct
       61 cgcgctgcgc ggaagcgatc tgcgtgatct gtgtgattcg cggttaaatt cattgtctgt
      121 caccccgccg caaccaggct ctctggtaca cgtccagacc gttcctgatg cacgcgctcg
      181 taaatcttgg cacgcgcgag aatctcatcg cagatcggcc ggtcgttatc gatcttcagc
      241 cacagtcgca gcaggtggcg cgtgtgctcg ggcgaatccc gataggcctc gcgcgcatgc
      301 agcatctgga aattgtgcca gagcagcatc tcgccgggct ccagaaggaa ctggatgccg
      361 atatcgtcgc gttccgccac ctcgctcatg tactcaatgg cttcggcaag ctttttcggg
      421 aacgtctcgc cacgcagctt cgccgcgcgt cgcatgaagt cgcgcacgaa gttgcagctc
      481 accacaccat cgacgttgca gaacaccggt atcttgaaat cggtcaccgg cttgctggag
      541 aactgcgcct ccgcgacagc gtaatgatac ccctcgtaga ggggctccag cagatccggc
      601 cgcgttgcaa ggatttcgtt gtggatcgcg aggctgctga cgatgcggct caggccaccg
      661 gtctccgccc gttgcaggca catgagcccg accacgcgat aggagtctgt gtgcggcgtg
      721 agttcctcat tgctgcgata gccgcgcgca accggatcgt ccttcacgtg ctgcacgtgc
      781 cccagccgat cgcccagcat gctttgcacc gcaggatcgc cgagacggac gccaataccc
      841 cagtagatac gctccatatc ctcttcgctg tgcgtcgcgc gtgtcaggcc tgacagcagc
      901 accgctccac gcccgcgccg tatcgtctcg tccagcccgg cgaccagtgc agaaatctcc
      961 ggatgtgcga aatcctccct tgtcgtctcc tgcggctgta aatgccgcgt ccgcgcaagc
     1021 gccgcatcaa acgccgcgag ttgcgctgcc gtcagacgct gcgtgaggcc ggccttcccg
     1081 ccgatgctcc gattggtcca cgccgccggt gcggtgatca cctcgcgata cggtgccatg
     1141 ccgtcctctc cctcaagctt cgcttccgtc ttcccggcca gcatggcaac gtcgcgggca
     1201 attcagcaag ggaggacgcc gccatgcgac tggccggcaa ggtagcgatc atcagtggtg
     1261 ccgcatccgg gatgggggcc gcgaccgccc gactgttcgc gcgcgaaggc gccaaggtcg
     1321 tcatcgccga cgtgctggag cacgaaggcg gacagatcgc cggcagtatc ggaagcgccg
     1381 cgcgcttcga acatctcgac gtcaccagcg aggacgactg ggcggccgtc gtctccgccg
     1441 ccacgacgca cttcggcaag ctggacatac tggtgaacaa cgccggcatt tccggcagtg
     1501 ccgaacagga cttttacagc accgaggcct ggcaccgcat catgggtgtc aacgcgaccg
     1561 gcgtcttctt cggcatcaaa cacgcagtcc aggcaatgaa ggccggtggc ggcggggcca
     1621 tcgtcaacct gtcgtcgatc gccgggatca tcggcagcga gcacgtgcac atggcgtata
     1681 acgcatcaaa ggcggccgtc cggctcatga caaaatccgt cgcggtgcag catgcgaagg
     1741 acaacatccg cgccaattcg gtgcacccgg gcatcatgcc agcgatgcgg acctctggcc
     1801 gcacggccga tcccgccatc cgcgcggaac ggatgcgcgt gatccctatg cgccgccccg
     1861 gtgaagtgga tgaggtcgcc tacgctatcc tgttcctggc atcagacgaa gcatcgtaca
     1921 tcaccggatc ggaaatccac gtggatggcg gcgcgatcgc gatctgacgc ggggccgccg
     1981 gcagcttatg gatcgacgca tcccacgccc gcctgggcat actcggtcca cccgcggtta
     2041 gctgcgaaag cgacccaccg gtgctgatcc gccactgaaa gcaccatcgt tgggcaggca
     2101 acattgaaca tcttcagtcc tgcgacgccg tacagggccc ggtcaggccg gaaacatccc
     2161 ggtccggcct gaggataacg cggccaccca tggttggctg cgaattcgct ccagcgctcg
     2221 cgttgcagag tggacgcgcc aggatcggag caggcgacat ggaagacata gatgtcggct
     2281 ggcattctct ggtccgactc ctgctgcatg gcgatctgat ccgccgtcat gaacaggcgc
     2341 tgcacgcaat agcctatgtc gtcagcgagg aatgccggac tgatcatcag gccgatcatc
     2401 agcagggcat gggctcgccg cgacaaatgc ggccaacgga atctcggcac cgcgacttgc
     2461 ggcacgagat gggggcgcgt cgaagccgtg ccggcatatg ccatttccgc ctccctgatg
     2521 cgggagctga ccgctcaccg cgtccgcctc gcgagcgcca ccttgccggc agtcgtcatc
     2581 acggcacgct ggattacact ccgcattcgg acgcgaggca agacaattga gcgaacgatt
     2641 cgccgtgttg tcctggcgct gctaacacat gcggacgggt ggcgtggcta ggttcgggga
     2701 agggtcatcg tgaccagccg gcacggtcaa cgcatggaaa cggatgtggc cgacctgcgg
     2761 atcagcgaag ttgctgacga aatcatcgaa atccagcatg ctgcggtgct ggaaggcgat
     2821 ttcgaaatcg acctcggcac caccttcacc gatccgaagg cgcgtgccga attctccagg
     2881 tgccctaccc ttgaagggct caggcattac cggttgcgcg atgtcacgct cgatgcatcg
     2941 acgatgctgc tcgttcgtga tcggcaacgc atcgccgaga cccgctacct ggttccggat
     3001 tcggcgtacg cagacatgct gaccaagccg ctgcacccga tgcctttgga tccggcggag
     3061 cattacatca tcggcggcaa ccgcgcctgg cataactatt tccattggat ggtccaggca
     3121 gtcccggcga tcgacatcgg cctgcgccat gccaatcacc ggcgggtcac gatcgttctg
     3181 ccgccagatc cgcgcccctg gcaggaggaa acccttgcat tgctgggata tcaggaggtt
     3241 ccccgctaca tcctgcacgg atcagggcat ttcctcctgc aaagcgctga attcagcgaa
     3301 ctcgtgggcg cgcgaggtcc tgagctcgtc gcccgtgtcg ccaccgccac ctacaagcgc
     3361 atgagccaag cggttccctg gacggaaccg ggtgccgagg aaatctatgt ggcgcgtacc
     3421 gactcgcaga accgcatcgc cgagaatgaa gccgagctga tggagatgct gcaacgccag
     3481 ggcgtgagca ttgtcgtacc cgggtcgctc actgtcagtg agcagattgc cacgttccgc
     3541 gccgcccggc tcgtcatcgg cccgcacggc gccggcatga gcaacatcgc gttctgccgt
     3601 cccggcagct tcgtctacga gatgctgccg cggcattatc caaacttcgc attcaaccgg
     3661 atcgctcagg cgagcggatt gaactacgtg gccgatatgt tcgagagctt cggatc
//
LOCUS       DATGEM010000373         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_167419, whole genome shotgun sequence.
ACCESSION   DATGEM010000373 DATGEM010000000
VERSION     DATGEM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167419"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VKQ27_11830"
     CDS             complement(<1..557)
                     /locus_tag="VKQ27_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89664.1"
                     /translation="MRAASAVISHMRAEPFAAVGTGIYVVILAMAVFAPHLAPYDPRA
                     ILRQGHRIARYLPISVEHWLGTTSSGQDVLSQLIYGARAALEVGLTAAIAVVAIGTLL
                     GLLAGYLGGWVDKVVTRVADIALGLPFLPFMLVLASLTAPGTRVVIVTVALLLWPNTA
                     RVIRAQVLSLRERAWVEAARVTGCST"
     gene            complement(544..1524)
                     /locus_tag="VKQ27_11835"
     CDS             complement(544..1524)
                     /locus_tag="VKQ27_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89665.1"
                     /translation="MRRLIAERLGHLLLVLWVIATLTFLLFRLMPGDPTLNFLSPTFT
                     DETRAALLKSFGLDKPLWEQYMLYMGNLLRGDFGRSFLQDAPVASILWDALPNTIILT
                     LVSLCIAYAFGIVAGAFLAFRRGGFVEAAAIPTALATRAAPDFWLGMLVLALFAFQLG
                     WFPTGGAVSPGATTGSLGHRLTSGDFWWHLCLPALTLTLFLQGLPLLLMRATMLEVMN
                     DEFVVMVRMKGLSRWSIVMRHAARNALLPVVTAFALGLGASIGSNVVIETVFAWPGIG
                     RVLVQAVQGSDYPVAQGAFIMIAFVLVVMNTVADITYALLDPRVSVGARR"
     gene            complement(1533..>2710)
                     /locus_tag="VKQ27_11840"
     CDS             complement(1533..>2710)
                     /locus_tag="VKQ27_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89666.1"
                     /translation="PFNVVAYNKTVWDPKSMISQAGIGIRCTWTWINIKPLGAQKDVI
                     LNSTAPPTNLNPFNIPGGQGSWATEIVWDRLMRVGPDGLAQPWAAEKVERPDATTVNV
                     TLRKGMKWSDGQPVTIDDAVFSLVAPGFADKAPMYKPFVANIASVEATGPDTLTIKLK
                     HPDAAFLVSSLSKLNLAPKHVWQPIFDDLKNKPQTAESIQEEHPVSSGPFRIVRASLN
                     QEIVLEANHDHWSHPTINRWIMRIMPNVEASVGALKSGEINFLSDYTGDPEVLKDLAK
                     SNPNIEVAQALDIGFKFIAYNERRPPFDNLAFRQAISAVIDRKLLAEDAWGGAAEPAN
                     SWISPALGVWHDKGIVDRVPGGNVDAAKKILKDAGFVAVDGKLHYPKGEKETTEAYH"
BASE COUNT          569 a          925 c          766 g          450 t
ORIGIN      
        1 gtcgagcaac ctgtaacgcg agccgcctcc acccaggcgc gctcgcgcag cgataacacc
       61 tgcgcacgga tcacccgcgc agtgttcggc cacagcagca gcgccaccgt cacgatcacc
      121 acccgcgtcc ccggcgcggt cagcgaggcc agcaccagca tgaacggcag gaacggcagc
      181 cccaacgcaa tatcggcgac ccgcgtcacg accttgtcca cccagccgcc aaggtagccg
      241 gccagcaagc ccagcaacgt gccgatcgcc accaccgcga tcgctgccgt caggcccact
      301 tccaaagcag cacgggcgcc ataaatgagc tggctcagca cgtcctgacc gctggacgtg
      361 gtgcccagcc aatgctccac cgatatcggc agatagcgag caatgcgatg cccctggcgc
      421 aatatcgcgc gagggtcata tggcgccaag tgcggcgcga acaccgccat cgccaggatc
      481 acgacgtaga tccctgtgcc gacagctgcg aacggctcag cccgcatatg gctgatgaca
      541 gcgctagcgg cgcgcaccga ccgataccct tggatcaagc agcgcgtagg taatgtcggc
      601 aaccgtgttc atcaccacca gcacgaaagc gatcatgatg aaggcgccct gagccacggg
      661 ataatccgag ccctgcacgg cctgcaccag cacacggccg atgccaggcc aggcgaacac
      721 cgtctcgatc accacattgc tgccaatgct ggcgcccagc cccaacgcga acgcggtcac
      781 caccggcaac aacgcattgc gcgccgcatg ccgcatcacg atcgaccagc gcgacagtcc
      841 cttcatgcgc accatcacca cgaattcgtc gttcattacc tccagcatcg tggcgcgcat
      901 caacagcagc ggcaggccct gcaggaacag cgtcagtgtc agcgccggca ggcaaagatg
      961 ccaccagaaa tcccctgacg tcagccgatg accaagcgaa cccgtcgtcg cgccaggact
     1021 gaccgcgccg cccgtcggaa accagccaag ctggaatgca aacagcgcca gcaccagcat
     1081 gccaagccag aagtcaggcg cggcccgcgt ggcaagcgcg gtcgggatcg ccgccgcctc
     1141 gacgaaacca ccccgcctga atgccaggaa tgcaccagcc acaatcccga acgcataggc
     1201 aatacagagc gatacaagcg tcaggatgat tgtattgggc agtgcgtccc acaggatgga
     1261 cgccactggc gcgtcctgca ggaacgatcg gccgaaatcg ccccgcagca gattgcccat
     1321 gtagagcatg tattgctccc acaacggctt atcgaggccg aagcttttca gcagcgccgc
     1381 gcgcgtctcg tcggtgaacg tcggcgacag gaaattcagc gtcgggtcgc ccggcatcag
     1441 gcggaacagc aggaacgtca gcgtggcgat cacccacagc accagcagca ggtgaccaag
     1501 ccgctccgca atcagacgcc gcatcgacag gctcagtgat aggcttcggt cgtttccttc
     1561 tcacccttcg gatagtgcag cttgccatca accgcgacga agccagcgtc cttcaggatc
     1621 ttcttcgctg catcaacatt gccgcccggc acgcgatcga cgatgccctt gtcgtgccac
     1681 acgccaagcg ccggcgaaat ccatgagttg gccggttctg ccgcaccacc ccaggcatcc
     1741 tcggccagca gcttccgatc gatcaccgcc gagatcgcct gccggaacgc gagattgtcg
     1801 aacggcggcc gccgctcgtt atacgcaatg aacttgaagc cgatatccag cgcctgcgcc
     1861 acttcgatgt tgggattgga ttttgccaga tccttcagta cctcaggatc acccgtatag
     1921 tcggacagga agttgatctc gccgctcttc aacgcgccca ccgatgcctc gacattcggc
     1981 atgatccgca tgatccagcg gttgatggtc ggatgcgacc agtgatcgtg attggcttcc
     2041 agcacgatct cctggttgag gcttgcgcgc acgatgcgga acggaccaga gctgaccggg
     2101 tgctcttcct ggatgctctc tgccgtctgc ggtttgttct tcaggtcgtc gaaaatcggc
     2161 tgccagacat gcttcggtgc cagattcagc ttcgacagcg aactgaccag gaacgccgca
     2221 tccggatgct tgagcttgat agtcagcgtg tcagggccag tagcctcgac gctcgcgata
     2281 ttggcgacaa acggcttgta catcggcgcc ttgtcggcaa agcctggcgc aaccaggctg
     2341 aacaccgcgt cgtcgatagt gaccggctgt ccgtcgctcc acttcatgcc cttgcgcagg
     2401 gtgacgttca ccgtggtggc atcaggacgt tccaccttct cggccgccca gggctgcgcc
     2461 aatccatccg gcccaacccg catcaggcga tcccagacaa tttcggttgc ccatgaaccc
     2521 tgcccgccag ggatgttgaa cggattgaga ttggtcggcg gcgcggtgga attcaggatc
     2581 acgtccttct gcgcgccgag cggcttgatg ttgatccatg tccaggtgca gcggatgccg
     2641 atgcccgctt gcgagatcat gctcttcgga tcccacaccg tcttgttgta ggccacaacg
     2701 ttgaacgggt
//
LOCUS       DATGEM010000374         7900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_461659, whole genome shotgun sequence.
ACCESSION   DATGEM010000374 DATGEM010000000
VERSION     DATGEM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461659"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VKQ27_11845"
     CDS             <1..202
                     /locus_tag="VKQ27_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89667.1"
                     /translation="DLGQGFGADLTEREIDWLANNEWARTAEDILWRRSKLGLRINAK
                     ALRTYLSSSNKIRFAAPSISSY"
     gene            complement(145..288)
                     /gene="ccoS"
                     /locus_tag="VKQ27_11850"
     CDS             complement(145..288)
                     /gene="ccoS"
                     /locus_tag="VKQ27_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HLY89668.1"
                     /translation="MNVILYLLGLGVLLGGGGLAVFLWALRSGQYDDMDGAANRILFD
                     DDK"
     gene            complement(285..2723)
                     /locus_tag="VKQ27_11855"
     CDS             complement(285..2723)
                     /locus_tag="VKQ27_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HLY89669.1"
                     /translation="MGADTDDLGGWAGDGCDAAGHRAVSVVELVREEAASPAGSCLHC
                     HLPMGAARQFCCPGCAAAYETIQALGLGRYYAQRVLDPALRAPRPELAEARDVTRHVA
                     TRADGSHELTLAVDGLQCGACVWLIESVLAREPDVLEGRVNLTTRRLRLVWRGLAARA
                     NSFVEAIEGLGYRLVPFSAEALAAAQDSTGRALLRALAVAGFAAGNVMLLSIGVWAGA
                     EHATRDLLHWVSALIAMPAIAYAGRPFFLSAWVVLRRGRTNMDVPISLGVVLVTGMSL
                     IQTIRGSEDAYFDSACALLFFLLIGRLLDHRARGQARATAEQLLMLRTADVAVVRDDG
                     HVERRGQESVATGDRVLVGMGERIGVDGVVERGASLLDTSLVTGESLPAPVTPGAAVF
                     AGTLNLGEALTVRSTATGGGTLLAECVRLIEAAEARRSRFVVLADRVARRYAPAVHVT
                     ALATFLWWYFVAGVPAGTALLTASAVLIVTCPCALALAVPVVQVIATGRLFRSGLLLK
                     SPTALERLADVDTVVFDKTGTLTEPVLALERGVGFDEAALQVAASLAVASRHPLARSL
                     VAAAGPVAMAEGAAEVPGQGVRARDVKLGSRSFVVTDDRVKPEDGRDEREGPEMWLAR
                     PGVAPVRFGFVETLREDAAATVARLRAMGVEVHLLSGDRASAVASVAQAIGIGTWQAE
                     RTPAQKVAAIEALMSAGRKVLMVGDGLNDGPSLAAATVSASPATAADVSQTVADVVFQ
                     GSRLAPVAAVIETARRARGVMRQNLALSIGYNVLMVPLAVAGFVTPWLAAAAMSSSSL
                     AVMLNSFRLRGGSI"
     gene            complement(2651..3151)
                     /locus_tag="VKQ27_11860"
     CDS             complement(2651..3151)
                     /locus_tag="VKQ27_11860"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HLY89670.1"
                     /translation="MSYIDWRDAKPQGRSAWRFFPWAVVGAIALVVAVNAGMVYAALH
                     SFPGAAEGDEGFALSNHYDVVLERAQHEAALGWVVSAQADDAGRAVVLLTDRAGAALH
                     GATVEATAERPLGAPDRRLLAFHEMAEGRYVADASLPTKGQWELTLTTSADGQAMAVT
                     RRVIVR"
     gene            complement(3148..4605)
                     /gene="ccoG"
                     /locus_tag="VKQ27_11865"
     CDS             complement(3148..4605)
                     /gene="ccoG"
                     /locus_tag="VKQ27_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HLY89671.1"
                     /translation="MARISKADRQLQLLREESGDHLYANRVRVYPKSVHGPVRNVKWT
                     VLIVCLGVYYLLPWLRWHRGVNEPTQAVLLSLWNERFYFFNLEFWPQDIYYLTGLLIM
                     GAVTLFLVTSLIGRVWCGYTCPQTVWTDLFMWIERLIEGDRNERMKRDAEPVSFDTVW
                     RKMAKHAAWLAIAFWTGGAWIMYYVDAPTVTVEFWSGTASREVYFFTFLFTATTYLLA
                     GWAREQVCTYMCPWPRFQSAMLDEQSITVTYQGWRGEPRTRGKRHGDARVGDCVDCGA
                     CVTACPTGIDIRDGIQLECINCGLCIDACNHVMERIGHPKWLITWDTLADQAAKAQGK
                     QTTLHLLRPRTLIYVSALAIAVIVMTGALLMRSSLGLSVLHDRAPLFVRLPDGDLRNG
                     YTVKIINKATAPVLYELRVNGLNGAILAEAEDGLGPASRLGLMVGADRVGTFRVTVAG
                     EPDRLVNGSQEVDFVLRNAMTGEQTVYHSVFMGPR"
     gene            complement(4615..5484)
                     /gene="ccoP"
                     /locus_tag="VKQ27_11870"
     CDS             complement(4615..5484)
                     /gene="ccoP"
                     /locus_tag="VKQ27_11870"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HLY89672.1"
                     /translation="MPTKIEKDTVTGTDTTGHEWDGLKELNTPLPKWWIYVFYATVAW
                     SFVWFVLYPSLPGVTGYFHGVLGYSQRAAVDQDVAAVMRQRAGAMDKIKTDSFAQIQQ
                     DPRLLAMADTAGRIAFANNCQPCHGAGGGGQPGYPALAAGAWIWGGTLPAIQQTITYG
                     IRSGDEKGRDTQMPRFGVDGIMKPAEIQQVADFVMTLYRHAEGGQAVSAGKKLFADNC
                     AVCHGDAGQGDREKGAPRLASRVHLYGDSRDIVVGQITNPRMGVMPTWHTRLDEATIK
                     SLTLYVHSLGGGE"
     gene            complement(5497..5643)
                     /locus_tag="VKQ27_11875"
     CDS             complement(5497..5643)
                     /locus_tag="VKQ27_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HLY89673.1"
                     /translation="MMTVISWLQHNWIVAVVAVFVLIVITTYWPGRKSLIERHGQIPL
                     EDDR"
     gene            complement(5646..6365)
                     /gene="ccoO"
                     /locus_tag="VKQ27_11880"
     CDS             complement(5646..6365)
                     /gene="ccoO"
                     /locus_tag="VKQ27_11880"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HLY89674.1"
                     /translation="MFIRHELIEKNAFLLLILTLLTVAIGGIVEVVPLFTIETTVEHV
                     KGVRPYSPLEQRGRDIYVREGCYLCHSQQIRALKDEVERYGHFSLAAESMYDHPFQWG
                     SKRIGPDLARVGGKYSSDWHYAHLKNAQGVVPESLMPHYAFLAETPLVTDDIQARLRT
                     LRTVGVPYSDEDVADAAADLRAQADPNGDPSALLKRYPKAVVVPGNGREVTEMDALVA
                     YLQVLGTMVDFADPTNEHLQQ"
     gene            complement(6369..7835)
                     /gene="ccoN"
                     /locus_tag="VKQ27_11885"
     CDS             complement(6369..7835)
                     /gene="ccoN"
                     /locus_tag="VKQ27_11885"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HLY89675.1"
                     /translation="MADVGIAMSHAAPEYNEAIVKKFLIASVFWGVAAFLVGVYIATE
                     LTWPSFNLGLSFINFGRLRPVHTSAAIFAFGGCALFATSFHVVQRTCRARLFGGEALA
                     NFVFWGYQFFIVMAALSYVMGFSQSQEYAEPEWHIDLWLTVVWVCYAIIFIGTLMKRE
                     EPHIYVANWFYLAFILTVAVLHIGNNIDIPISVFSAKSYPVAAGVQNAMIQWWYGHNA
                     VGFFLTAGFLGMMYYFIPKAANRPVYSYRLSVVNFWSLIFLYIWAGPHHLHWTALPDW
                     TQTLGMAFSVMLWMPSWGGMINGLMTLSGAWDKLRTDPSLRFSVTAVGFYGMSTFEGP
                     VMSVRDVNALSHYTDWTIGHVHSGALGWVAFISFGAIYYLVPVLWKRSSLYSTRLVAW
                     HYWIATLGIVFYITSMWVAGIMEGLMWRAYDQFGFLQYSFAESVVAVHPYFVIRLLGG
                     LMFLSGALLMVFNLIMTVRSSSTERAIAPQLAAVAAGE"
BASE COUNT         1618 a         2900 c         2123 g         1259 t
ORIGIN      
        1 agaccttggc caaggcttcg gagcggacct gaccgaacgc gagattgatt ggctggcaaa
       61 caacgaatgg gcgcgcacgg cagaagatat actctggcga cgcagcaagc tcggattgcg
      121 catcaacgcg aaagcgctca ggacctactt gtcatcgtca aacaagatcc ggttcgctgc
      181 accgtccata tcatcgtatt gaccggatcg cagcgcccac aggaacaccg ccagtccgcc
      241 gccgccaagc agcacgccca agcccagcaa atacaggata acgttcatat cgagccgcct
      301 cgcaggcgga agctgttgag catcaccgcc agagacgaac tcgacatcgc cgccgccgcc
      361 agccatggcg tgacgaatcc cgccaccgcg agcggcacca tcagcacgtt atacccaatg
      421 gacagcgcca aattctgccg catgacgccg cgcgcacgcc gcgccgtctc gatcaccgca
      481 gccacgggtg ccagccggga gccctggaac accacatccg ccaccgtctg gctgacatcg
      541 gccgccgtcg ccggcgacgc tgacactgtc gcggcagcaa gcgagggccc atcattcaac
      601 ccatccccca ccatcagcac cttgcgcccc gcagacatca acgcctcgat cgcagccacc
      661 ttctgcgccg gcgttcgctc tgcctgccac gtgccaatcc cgatcgcctg cgccaccgac
      721 gccacggcgc tggcgcggtc gcccgacaac aggtgcacct ccacccccat cgcccgaagc
      781 cgcgctaccg tcgcggctgc atcttcgcgc aacgtctcca caaacccgaa ccgcaccggc
      841 gctacaccag gtcgagccag ccacatctcc ggcccctcgc gctcgtcacg cccgtcctcg
      901 ggcttaaccc ggtcatctgt cacaacgaag ctccggctcc cgagctttac atcccgagcc
      961 cgaacccctt gcccaggcac ctctgccgca ccctcggcca tcgccaccgg gccagccgcc
     1021 gccaccaggg atcgagcaag cggatgtcgg ctcgccacgg ccagcgacgc cgccacctgc
     1081 aacgccgcct catcgaagcc gacaccgcgt tccaacgcga gcaccggttc agtcagcgtc
     1141 cccgtcttgt caaatacaac cgtatcgaca tccgccagcc gctccagcgc cgtcggcgac
     1201 ttcagcagca agcccgagcg gaatagccgc cctgtcgcga tcacctgcac cacgggtacc
     1261 gccagcgcca atgcgcaggg acaagtaacg atcagcaccg cggatgcggt cagcagcgca
     1321 gtccccgccg gcacacccgc cacgaaatac caccacagga atgtcgccag cgctgtcaca
     1381 tgcactgccg gcgcgtaccg gcgcgccacc cggtccgcca gcaccacaaa tcgggaccgt
     1441 cgagcctccg cggcctcgat aagccgcacg cattcggcca acagcgtgcc gccgccggtc
     1501 gcggttgacc gcactgtcaa agcctcgccc aggttcagtg tgccggcaaa cactgccgcg
     1561 cccggcgtca ccggggctgg caggctctct cccgtcacca gacttgtatc cagcagagac
     1621 gcgccccgtt ctacaacgcc atccacgcca atccgctcgc ccatgccgac gagcacccgg
     1681 tcgcccgtcg cgacgctctc ctgcccacgc cgctccacat gcccatcgtc gcgcaccacc
     1741 gcgacatcag ccgttcgcag catcagcaac tgctccgccg tcgcccgagc ctggcctcgc
     1801 gcgcgatgat ccagcagtcg cccgatcagc aaaaagaaca gcagtgcgca ggcggaatcg
     1861 aaatacgcgt cttcgctgcc ccgtatggtc tgtatcagac tcattccggt caccagcaca
     1921 acgcccaggc tgatcggcac atccatgttg gtgcgtccgc gccgcagcac cacccacgcg
     1981 gacaaaaaga acggcctccc cgcataagcg atcgccggca tcgcgatcag cgccgacacc
     2041 cagtgcagca gatcgcgcgt cgcgtgctcc gctcccgccc acaccccgat cgacagcagc
     2101 atcacattgc ccgcggcgaa ccctgcgacc gccagcgccc gaagcaacgc ccgcccggtg
     2161 ctatcctgag ccgccgccag cgcctcggca ctgaatggca cgagccggta gccgaggccc
     2221 tcgatcgcct ccacaaacga attggcacgc gccgccaagc cgcgccacac caggcgcagc
     2281 ctccgtgtgg tcaggttgac gcgtccctcc agcacgtccg gttcgcgcgc cagcacggac
     2341 tcgatcaacc acacgcaagc cccgcattgc agcccatcta ccgccagcgt cagctcatgg
     2401 ctgccatccg cgcgcgtcgc gacatgccgt gtgacatccc gcgcctccgc caactcgggt
     2461 cgaggcgccc gcagcgccgg atcgagcacc cgctgtgcat agtatcgccc cagcccaagc
     2521 gcctggatcg tctcataggc cgccgcgcac ccggggcagc agaactgacg cgcggcgccc
     2581 ataggcagat ggcaatgaag gcacgaaccg gccggagaag ccgcctcctc gcggaccagc
     2641 tcgacgacac tcaccgcacg atgacccgcc gcgtcacagc catcgcctgc ccatccgccg
     2701 aggtcgtcag tgtcagctcc cactgcccct tcgtcggcaa cgatgcatcg gcaacatacc
     2761 gcccctccgc catctcatgg aacgccagca gccgcctgtc cggcgcaccc agcggccgct
     2821 ccgcggtcgc ctccactgtt gccccgtgca gtgccgcacc tgcccggtca gtcagcaaaa
     2881 ccaccgcccg tcccgcgtcg tccgcctgtg cggatacaac ccaccccagc gctgcctcat
     2941 gctgcgcgcg ttccagcacg acgtcgtagt gattgctcag cgcgaacccc tcatcccctt
     3001 cggctgcacc cggaaagctg tgcaatgccg cgtagaccat cccggcattc accgcgacga
     3061 ccagcgcgat cgcaccaacc accgcccatg gaaagaaccg ccatgccgag cgtccttgcg
     3121 gctttgcatc cctccaatcg atgtagctca tcgtggcccc atgaatacag aatgatacac
     3181 agtctgctca ccggtcattg cattgcgcag gacgaaatcc acctcctgcg agccattcac
     3241 cagccgatcc ggttcgcccg ccaccgtcac ccggaaggtg cctaccctgt cggctccaac
     3301 catcagcccc agccggctcg ccggcccaag cccatcctcg gcttccgcca ggatcgcgcc
     3361 attcaggcca ttgacccgca gctcatacag cactggtgcc gtagccttgt tgatgatctt
     3421 cacggtgtag ccattacgca gatccccgtc cggcaaccgc acgaacagcg gcgcgcgatc
     3481 atgcagcacc gacaacccca gagacgaccg catcagcagc gcgccggtca tcacgatcac
     3541 ggcaattgcc agcgccgaga catagatcag tgtccgaggc cgcagcagat gtagcgtcgt
     3601 ctgcttcccc tgcgccttcg ctgcctggtc agccagcgtg tcccaggtga tcagccactt
     3661 aggatgcccg atgcgctcca tcacgtggtt gcacgcatct atacacagcc cgcagttgat
     3721 gcactccagt tggatgccgt cgcggatgtc gatcccggtc gggcatgccg tgacacaagc
     3781 gccacaatcc acgcaatcgc ccacccgcgc gtcgccatgt cgcttgccac gtgtccgggg
     3841 ttcaccgcgc cagccttggt acgtgaccgt gatgctctgc tcatccagca tcgccgattg
     3901 gaaccgtggc cacgggcaca tgtacgtgca cacctgctcg cgcgcccagc cagccagcag
     3961 ataagtggtc gcggtgaaca ggaacgtgaa aaagtacact tctctggacg cggtgccgct
     4021 ccagaactcc accgtcaccg tcggcgcatc gacgtagtac atgatccacg cgccgccggt
     4081 ccagaacgcg atcgccagcc acgccgcgtg cttcgccatc ttgcgccaca ccgtgtcgaa
     4141 gctcaccggc tccgcatcac gtttcatgcg ctcgttgcgg tcaccttcga tcagccgctc
     4201 gatccacatg aacagatcgg tccacaccgt ctgcgggcaa gtatagccgc accacacgcg
     4261 gccaatcagg ctggtaacca ggaacagtgt gaccgcaccc atgatcagca ggccagtcag
     4321 gtaatagatg tcctgcggcc agaactcgag attgaagaaa tagaaccgct cattccacag
     4381 cgacagcagc accgcctgcg tcggctcatt cacgccacga tgccagcgca gccacggcag
     4441 cagatagtag acgcccagac agacgatcag caccgtccac ttgacgttgc gcaccggccc
     4501 gtgcaccgat ttcgggtaga cgcgcacgcg attggcatac aggtgatcgc ctgattcctc
     4561 gcggagcagc tggagttgcc ggtcagcctt gctgatgcga gccatggcgt cgcttcactc
     4621 cccgcctccc agcgaatgca catacagcgt caggctcttg atcgtcgcct catccagccg
     4681 cgtatgccac gtcggcatca cgcccatccg agggttggtg atctgtccca ccacgatgtc
     4741 gcgcgaatcg ccgtacagat gcacgcgcga tgccaggcgc ggcgcaccct tttcgcgatc
     4801 accttgcccc gcatcgccat ggcacacagc gcagttgtcg gcgaacagct tcttcccggc
     4861 cgacacggcc tgcccaccct cggcatgtcg atacaaggtc atgacaaagt cagcgacttg
     4921 ctggatctca gccggcttca taatcccatc cacgccgaac cggggcatct gtgtatcgcg
     4981 ccccttctcg tcaccgctgc gaatgccata cgtgatggtc tgctggatcg ccggcagcgt
     5041 cccgccccag atccacgcgc cagccgcaag cgccggatag cccggctgtc cgccaccgcc
     5101 cgcgccatga cacggctggc agttattggc gaacgcaatg cgccccgcgg tgtccgccat
     5161 cgcgagcaac cgcgggtcct gctggatctg cgcgaacgag tccgtcttga tcttatccat
     5221 cgcgccggcg cgttgcctca tcaccgccgc cacgtcttga tccacagccg cgcgctgcga
     5281 ataacccagc actccgtgga aatacccggt cacacccggt aacgatggat acagcacgaa
     5341 ccacacgaat gaccaggcaa cggtggcata aaacacatag atccaccatt tcggcagcgg
     5401 tgtgttcagc tccttcagcc cgtcccattc atggccggtc gtgtccgtgc cggtgacggt
     5461 gtccttctcg atcttggtgg gcacgatttc gcctcctcag cgatcgtctt cgagcgggat
     5521 ctgtccgtgc cgctcgatca gggacttgcg tcccggccag taggtcgtga tcacgatcag
     5581 cacgaacacg gcaaccaccg ctacgatcca attgtgctgc agccaggaaa tcacggtcat
     5641 catggctatt gctgcaggtg ttcgttggtg ggatccgcga aatccaccat cgtgccgagg
     5701 acctggagat acgcgaccag cgcatccatc tccgtcacct cgcgcccgtt acccggaacc
     5761 accaccgcct tcggataccg cttcagcagc gcgctcggat ccccattcgg atcagcctgc
     5821 gcccgcagat cagccgctgc gtcggcaaca tcctcatcgg aatacggcac accaaccgtc
     5881 cgcagtgtcc gcagccgcgc ctggatgtcg tccgtcacca gcggcgtctc agcaaggaac
     5941 gcatagtgcg gcatcagcga ctccggcacc acgccctgtg cattcttcag atgtgcgtaa
     6001 tgccagtcac tcgaatactt gccgccgaca cgtgccaggt ctggcccaat ccgcttgctg
     6061 ccccactgga acggatgatc gtacatgctc tccgccgcga gcgagaaatg gccatagcgt
     6121 tccacttcat ctttcagcgc gcggatctgc tggctgtggc acagatagca gccctcgcgc
     6181 acatagatgt cacgcccgcg ctgctccagc ggcgagtaag gccgcacacc cttcacatgc
     6241 tccaccgtgg tttcgatggt gaacagcggc accacctcga cgataccgcc gatggccacc
     6301 gtcaacagcg tcaggatcag tagaagaaac gcattcttct cgatgagttc gtgacggatg
     6361 aacatctgtt attctcccgc cgcgacggct gccaactgcg gcgcgatcgc ccgctcggtc
     6421 gatgagctgc gcacagtcat gatcagattg aacaccatga gcagtgcacc gctcaggaac
     6481 atcagcccgc ctagcaatcg gatcacgaag tacgggtgca ccgcgaccac cgattctgcg
     6541 aacgagtact gcaggaagcc gaactggtcg taggctcgcc acatcaggcc ttccatgatg
     6601 ccggcaaccc acatcgaggt gatgtagaat acgataccca gcgtcgcgat ccagtagtgc
     6661 cacgccacca gtcgcgtgga gtacagactg ctgcgcttcc aaagcaccgg caccaggtaa
     6721 tagatcgcgc caaaactgat gaacgccacc cagccgagtg caccggaatg cacgtgcccg
     6781 atcgtccagt cagtgtagtg cgacagcgcg ttgacatcgc gcaccgacat caccggccct
     6841 tcgaaggttg acattccgta gaagcccacc gcggtaaccg agaagcgcag cgacggatca
     6901 gtacgcagtt tgtcccacgc gcccgacagc gtcatcagcc cgttgatcat gccaccccac
     6961 gatggcatcc acagcatgac ggaaaacgcc atgcccagcg tctgcgtcca gtcaggcagg
     7021 gcggtccagt gcaggtgatg cgggccggcc catatataca ggaaaatcag cgaccagaag
     7081 ttcaccaccg aaagccggta agaatacaca ggacggttcg ctgccttcgg gatgaagtag
     7141 tacatcatcc ccaggaaccc agcggtcagg aagaagccca cggcattgtg gccataccac
     7201 cactggatca tggcgttctg cacaccggcg gccaccggat agctcttggc cgaaaacacc
     7261 gagatcggaa tgtcgatgtt gttgccgata tgcagcacgg caaccgtcag gatgaaggcg
     7321 agatagaacc agttcgccac gtagatgtgc ggttcctcgc gcttcatcag cgtgccgatg
     7381 aagatgatcg cgtagcagac ccagacgacg gtcagccaca ggtctatatg ccattcaggt
     7441 tcggcatatt cctggctctg gctgaacccc atcacgtagg acagcgccgc catgacgatg
     7501 aagaactggt agccccagaa cacgaaattg gccaaagcct cgccgccgaa cagccgcgct
     7561 cggcatgtcc gctgcacgac atggaacgac gttgcgaaca gcgcgcatcc gccaaaggcg
     7621 aagatcgccg ccgacgtgtg caccggccgc aggcgcccga aattgatgaa cgaaaggccc
     7681 aggttgaacg agggccatgt tagttcagtc gcgatataaa cgccgaccag gaatgccgcc
     7741 acgccccaga acaccgaggc gatcaggaat ttcttcacaa tcgcctcgtt gtattccggt
     7801 gcggcatgcg acatggcgat gccgacgtca gccatgtcgg cgtgcctctg ccttgtcgaa
     7861 gtgccggcgg agcagaccga actcgaacac cgtggcgaag
//
LOCUS       DATGEM010000375         7939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_478183, whole genome shotgun sequence.
ACCESSION   DATGEM010000375 DATGEM010000000
VERSION     DATGEM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478183"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VKQ27_11890"
     CDS             <1..570
                     /locus_tag="VKQ27_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLY89676.1"
                     /translation="SRGAPVEKRERAGLLLLALASLLLRGGERVTIIAPDQRPIAGRA
                     GLDRLADSLELLSNDDAGLPPQVPLPRHARVVLLGDFLSPLSEIQAAVGRLAAIPVSG
                     HLLQVLDPAEADLPYVGRIRFRGLEREGDTLIPRVENVRQEYGQRLKAQQEGLSAICA
                     AAGFGFAIHRTDHSPEAALLGLYMGLTAR"
     gene            629..3313
                     /locus_tag="VKQ27_11895"
     CDS             629..3313
                     /locus_tag="VKQ27_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HLY89677.1"
                     /translation="MIFAAPWVLLALPALPLLWWLLRVTPPAPRSETFPAIRLLMGLH
                     AREETPARTPWWLLLLRMTVATLVIVALARPILDAGSSLPGSGPLLLVVDNGWASAGD
                     WPRRMQAANTVLDRAERAGRQVALLTTAPDETGAPLQATAPMPVADLRSRLAALHPEP
                     WPPDRAGAVAPLRAWHHPDTAVVYLGDGLTHGDDFAPFAAALSAAGSVTELRNPAAAA
                     RLMLPPGNEADRLIARVRQVPQPLPGQAVVLAQSGDGRTLARATIDLPPGADEGSAAI
                     TLPPELRNRLSRLVLQGPPTAASLVLLDERWRRRPVGLLAGDLATADTPFAGPLYFLR
                     RALAAYTEVREADLNSLLSRDLSVLILADRPLPAGPDRDAVEAWVAKGGLLIRFAGPR
                     TAEQPIGESDPLLPVHLLGGDRQLGGALSWAEPAGVAQFAAGSPFAGLQVPAEVKVTR
                     QVLAEPSADLASHTWATLADGTPLVTEAAHGAGRVVLFHVTANADWSNLPLSGLFVEM
                     LRRLVALSAGVSTPADNTVLAPAETLDGYGVLSTPPAAATGLPADAVATTKASPRHPP
                     GLYGPENGRRALNLGANLSAPAAAPAISGARVEDYAAAVPERALGPSLLAAALVLLAL
                     DMLIALGLRGLLRSTRVAAGVLLILLAAPAAHALDTDTNPALATRLGYVVTNDPQLDN
                     IARTGLEGLSDYVNRRTAATLVEPDPVEPGKTDLSFYPLLYWPISADAQPLTPPQAAA
                     LNDYMSRGGIILIDTRDSGSGAGFAPGTETALQRVARGLIIPPLAPLTTEHVLAHAFY
                     LLQDFPGRYTGDTVWVQRDQDRTNDSVSPVIIGGNDWAAAWATDDAGRNPYAVIPGGE
                     RQRTLAYRFGVNLVMYALTGNYKGDQVHVPAILQRLGQ"
     gene            3310..5415
                     /locus_tag="VKQ27_11900"
     CDS             3310..5415
                     /locus_tag="VKQ27_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89678.1"
                     /translation="MRPEEVIAALRFEPLLPVWAIAALGVLALLIVLLAAFRRARGTI
                     LRAAGFAILVLWLAGPRIVQESRENLPDIGLLVVDQTASMQIGDRTRLTDAARAAIQQ
                     QAGKLRDLELRTITVPESGDSGTRLFSSIDRALADIPRSRLAGTIAITDGQVHDIPDT
                     APGGAPLNVLVPARGEETDRHLRVIEAPSYGIVGKTATIRVAIDDLGVAHPTGSANLT
                     IRRDGDPPTVENVPIGVEQTIGIPITRAGPTVIEMAANPLPGEVSTINNRAVVEINGV
                     RDRLRVLLISGEPHPGERTWRRLLKADPSVDLVHFTILRPPEKDDLTPLNELALIAFP
                     VRELFLDKINEFDLIILDRFQNRGLLPLPYIANIADRVRDGGALLLSVGPEFSGASSL
                     AATPLGSILPAYPAQAHAVVDEKFRPRVTDLGQRHPVTEGLTGANPAGKADVPPSWGS
                     WYRHIDATGLHGEDLMDTRDGDPLLLLNRVGKGRVALLLSDQIWLWSRGHEGGGPQAE
                     LLRRVAHWLMKEPELEENTLTAHVADGRLTIERRSTDPAPPGAVTVTDPDGKAQSLQL
                     TPSAPGRATVTLPATTPGVWQATDGTHAAYAAAGAANPLEFADLRATATPLRKLARTS
                     GGGVHFIGTGKPGAAPDVPELRRTEPDRPAAGGSWIGLERRHDHVVTGIASLTLLPAW
                     AALPLMLGLLVAGWRREGT"
     gene            complement(5483..5863)
                     /locus_tag="VKQ27_11905"
     CDS             complement(5483..5863)
                     /locus_tag="VKQ27_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89679.1"
                     /translation="MKYTWEHIHLRSPDPAATADWYADKLGAEVIRTSQPDGSTRIDL
                     DLTGQKIFIAKADPAMTGASPQTPYMGLEHFGLTVPDIDAAVAELKAKGVAFTMEPKT
                     IRPGVRIAFLTAPQNVSIELIQRG"
     gene            complement(5873..7081)
                     /locus_tag="VKQ27_11910"
     CDS             complement(5873..7081)
                     /locus_tag="VKQ27_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89680.1"
                     /translation="MAQPEAHNFRLISQDLMSGFGNVGEGMSLQLAKGGRRIMWMAHE
                     CAPKNFTAVEVTDPRKPKVIVQTDLPHRNVRSNSLEICGDVMAVAYQTGTHGATPAGI
                     ELFDISTPEEPKRIAFIDRSGPRSRGVHQLWFIDGKTIHCSSGAADFMPRNLKDDQCY
                     TAFDVRDPTKPKELGRWWYPGTEERDAAPPVPRHPKFDTGWRAHNTNVYPERPDRAYV
                     GYIDGGACVLDISDIGAPKLVGHWNPHPPFPGFTHTVMPLFDRDLYVVSDECVRTEGA
                     DWPKLVWLLDARREDNLVSMATLPIPPMEDYALGGRYGAHNLWENRPGPAFQSSTLVF
                     GTYFGGGLRVHDISDPLQPKEVASFIPEPPEGSPMKAGMINDVYVDENRLVYTVDRFA
                     GGLYVLEMTM"
     gene            complement(7117..7533)
                     /locus_tag="VKQ27_11915"
     CDS             complement(7117..7533)
                     /locus_tag="VKQ27_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLY89681.1"
                     /translation="MRQGEAVFVDSAAWIALALSRDPLHEQAREHWELLQGVGAALHT
                     SIPVIIETFTFLDRNANRDVALAWKEAIYKPRAVKVLACELRDLQQSWEYFRRRDLHK
                     LSAVDASSFAIMRRARIRLAYTFDHHFAVVGFRLVG"
     gene            complement(7533..7742)
                     /locus_tag="VKQ27_11920"
     CDS             complement(7533..7742)
                     /locus_tag="VKQ27_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HLY89682.1"
                     /translation="MVKTQVYLRQEELDALREIAARSGCSVAELVRDAVRKTVLSSRA
                     SGPVALWDGEPKRSSIEHDSIYDDV"
     gene            7859..>7939
                     /locus_tag="VKQ27_11925"
     CDS             7859..>7939
                     /locus_tag="VKQ27_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLY89683.1"
                     /translation="MSEAANTVPVTILTGFLGSGKTTVLNH"
BASE COUNT         1258 a         2928 c         2402 g         1351 t
ORIGIN      
        1 tcgcggggcg ctcccgtcga gaagcgcgaa cgtgcgggcc tgctattgct ggcgctggcc
       61 tcgctgctgc tccgcggcgg cgagcgcgtg accatcatcg ctcccgacca gcgcccgatt
      121 gccggacgcg ccgggctcga tcgcctcgcg gactccctgg agctcttgtc aaacgatgat
      181 gccggcctgc cgccgcaggt ccccttgccg cgccacgccc gcgtcgtgct ccttggcgat
      241 ttcctgtcgc ccctgtccga aatccaggcg gcggttggac gcctcgccgc cattccggtc
      301 agcggccacc tgctccaggt tctggatccc gccgaagccg atcttcccta tgtcggccgt
      361 atccgctttc ggggactgga gcgcgaaggc gacaccctga tcccgcgcgt ggaaaacgtg
      421 cggcaggaat acggccagag actgaaagcc cagcaggaag gcctttcggc aatctgtgct
      481 gctgccgggt ttggatttgc cattcaccgc acggatcatt cacccgaagc ggctttactc
      541 ggtctatata tgggcctgac agcacgatga cgaccgggac aggcccggtg gtagcctctg
      601 aaggccgggc tggggcccgg gcgtccgtat gatctttgcc gcgccctggg ttctcctggc
      661 acttccggcc ttgccgctgc tctggtggct gctccgtgtc acgcccccgg caccgcgcag
      721 cgagaccttc ccggcgatcc gcctgctcat gggcctgcat gcgcgcgagg agacgccggc
      781 gcgcacgccc tggtggctgc tgctactgcg catgaccgtc gcgacgctcg tcatcgtggc
      841 cctggcgcgg ccaatcctcg atgccggatc cagtctgccg ggcagcggtc cgctgctgct
      901 ggtggttgac aacggctggg cctccgccgg tgactggccg cgccggatgc aggcggcgaa
      961 tacagtgctg gaccgcgccg agcgggcggg ccgccaggtt gccctcctga ccaccgcgcc
     1021 cgacgagacg ggcgcaccgc tgcaggccac tgctcccatg cctgtcgccg acttgcggtc
     1081 gcgcctggcc gcgctgcatc cagaaccgtg gccgcccgat cgcgctggcg ccgtggcacc
     1141 gctgcgggcc tggcatcacc ctgataccgc cgtggtttat ctcggcgacg gcctgacgca
     1201 cggcgacgac ttcgccccgt ttgccgccgc gttgagcgcg gcaggttcgg tgactgaatt
     1261 gcgcaacccg gccgctgcgg cgcgtctgat gctgcccccc ggcaacgagg ccgacaggct
     1321 gatcgcccgc gtgcggcagg tgccacagcc gctcccgggt caggctgtcg tgctggcgca
     1381 gagcggcgac ggcaggacgc ttgcgcgcgc caccatcgat ctgccgccgg gcgccgacga
     1441 gggcagcgct gcgatcacat tgccgccgga actccgcaac cgtctaagcc ggttggtgct
     1501 gcaaggacct ccaacagccg cctcgctggt cctgctggat gagcgctggc ggcggcggcc
     1561 cgtcggcctg ctcgccggcg atctcgccac cgctgatacg ccgttcgcgg gcccgctgta
     1621 tttcctgcgc cgcgcactgg ccgcgtatac cgaggtccgc gaagccgacc tcaacagcct
     1681 tctgagccgt gacctttcgg tgctgatctt ggcggaccgg ccgctgccgg ctggccccga
     1741 ccgcgatgcg gtggaggcct gggtcgccaa gggcgggctg ctcatccgct tcgccggccc
     1801 gcgcactgcc gagcagccga tcggcgagag cgaccctctg ctgcccgtgc acttgctggg
     1861 cggcgatcgc cagcttggcg gcgcgctgtc gtgggccgaa cccgcgggcg ttgcccagtt
     1921 cgccgccggc tcgccattcg ccggcctgca agtcccggcc gaggtgaagg tcacccgcca
     1981 ggttcttgcc gaaccctcgg ctgacctcgc ctcccacacc tgggcgacgc tcgccgacgg
     2041 cacgccactg gtgaccgagg ccgcacacgg cgccggccgc gtcgtgctct tccacgtcac
     2101 cgcgaatgcc gactggtcca acctgccgct ctccggcctg ttcgtggaaa tgctccgccg
     2161 tctggtcgcc ttgtccgccg gcgtcagcac cccggccgac aacaccgtcc tggcgccggc
     2221 tgagacgctg gacggctatg gcgtgctctc gacgccgccc gccgcggcca ccggcctgcc
     2281 agctgacgcg gtcgccacca ccaaggcgtc gccacgccat ccaccgggcc tctacggacc
     2341 cgagaacggc cgccgggcgc tgaaccttgg cgcaaaccta tccgccccgg ccgcggcacc
     2401 cgcaatatcc ggcgcgcgcg tcgaggacta cgccgccgcc gttccggaac gcgcgctcgg
     2461 accgtccctg cttgccgcgg ccctggtgct gctggcactc gacatgctga tcgccctggg
     2521 cctgcgcggc ctgctgcgct cgacgcgcgt ggccgccggc gtcctgctga tcctgctggc
     2581 cgcacccgcg gcccatgcgc tcgacaccga caccaatccg gccctggcga cccgtctcgg
     2641 ctacgtcgtg accaacgatc cgcagttgga caatatcgcg cgcaccggcc tggagggact
     2701 gtccgactac gtcaaccgcc gcacggcggc gacgctggtc gaaccggatc cagtcgagcc
     2761 cggcaagacc gatctcagct tctacccgct gctctactgg ccgatcagcg ctgacgcgca
     2821 gccgttgacg ccgccacagg ccgccgcgct caacgattac atgagtcgtg gcggcataat
     2881 cctcatcgac actcgcgatt ccggttctgg tgccgggttt gctccgggca ccgagacggc
     2941 cctgcagcgc gttgcccgtg gcctgatcat tccgccactg gccccactca ccaccgagca
     3001 cgtgctcgcc cacgccttct acctgttgca ggattttccc ggccgctaca ccggcgatac
     3061 ggtctgggtg cagcgcgacc aggatcgcac gaatgacagc gtcagcccgg tgatcatcgg
     3121 cggcaatgac tgggccgccg cctgggcaac ggatgacgcg ggccgcaacc cttatgccgt
     3181 gatccccggt ggtgaacgac agcgcactct ggcctaccgc ttcggcgtca atctcgtgat
     3241 gtacgcgctg accggaaact acaaaggcga ccaggtccac gtgccggcca tcctgcaaag
     3301 gctggggcaa tgagaccgga agaagtcatc gccgcactgc gcttcgagcc gctgctcccg
     3361 gtctgggcaa tcgccgctct cggcgtgctc gctctcctta tcgtcctgct cgccgccttc
     3421 cgccgcgccc gaggcaccat cctgcgcgcc gccggcttcg ccatcctggt gctctggctc
     3481 gccggtccgc gcatcgtgca agaaagccgc gagaaccttc ccgacatcgg gctccttgtc
     3541 gttgaccaga ccgcgtcgat gcagattggc gaccgcaccc gcctgaccga cgccgcacgc
     3601 gccgcgattc agcagcaggc cggcaagctc cgcgatctcg aactccgcac catcaccgtc
     3661 ccggaaagcg gcgattcagg cacccggctc ttctcctcga tcgaccgcgc cctcgctgac
     3721 attccgcgtt cacgcctcgc cggcactatc gcgatcacgg atggccaggt gcacgacatt
     3781 ccggacaccg cacccggcgg cgcacccctc aacgtgctgg taccggccag aggtgaggag
     3841 accgatcgcc acctccgcgt gatcgaggcc cccagctacg gcatcgtcgg taagaccgcc
     3901 acgatccgcg tcgcgatcga cgatctcggc gtagcgcatc ccaccggctc cgccaatctc
     3961 acgatccgcc gggacggcga tccgccaacg gtggagaacg tccccatcgg cgtcgaacag
     4021 acgatcggca tcccgatcac ccgtgccggt cccacggtga tcgaaatggc cgccaacccg
     4081 ctccccggcg aggtgtctac catcaacaac cgcgccgtcg tcgaaatcaa cggcgttcgc
     4141 gaccgcctgc gcgtgctgct gatctcgggt gagccacatc ccggcgaacg cacctggcga
     4201 cgcctgctga aggccgaccc ttcggttgac ctggtgcatt tcaccatcct gcgcccgccc
     4261 gagaaggacg acctcacccc gctcaacgaa ctcgccctga tcgccttccc ggtgcgcgag
     4321 ctgttcctcg acaagatcaa cgagttcgac ctgatcatcc tggaccgctt ccaaaaccgc
     4381 ggcctgctgc cgctgccata tatcgccaac atcgctgatc gcgtgcgcga cggtggcgcg
     4441 ctgctgttga gcgtggggcc ggagttctcc ggcgccagca gcctcgccgc tacgccgctt
     4501 ggttcgatcc tccccgccta ccccgcccag gcgcacgccg tggtggacga aaagttccgc
     4561 ccgcgggtga cggaccttgg ccagcggcat ccggtgactg aaggcctcac gggcgcaaac
     4621 ccggccggca aggccgatgt gccgccgagc tggggatcct ggtaccgcca catcgacgcc
     4681 actggcctgc acggcgagga cctgatggac acgcgcgacg gcgatcctct gctgttgctg
     4741 aaccgcgtcg gcaagggccg tgtcgcactg ctgctctccg accagatctg gctgtggtca
     4801 cgcggccacg aaggcggcgg cccacaggct gaactgctgc gacgcgtcgc acactggctg
     4861 atgaaagagc cggagctgga ggagaacacc cttaccgccc acgtcgccga tggccgcctg
     4921 acgatcgagc ggcgctcaac cgatcccgcc ccgccaggcg ccgtcaccgt caccgatccc
     4981 gacggcaagg cccagtcatt gcagctcact ccgtccgcac cgggccgcgc caccgtaacg
     5041 ctgccggcca cgactcctgg cgtctggcag gccaccgacg gcacgcacgc agcctacgcc
     5101 gccgccggcg ccgccaatcc gctggagttc gccgaccttc gcgccaccgc cacgccgctg
     5161 cgcaaactgg cacgcacctc gggtggtggt gtccacttca tcggcacggg caagccgggc
     5221 gccgcacccg acgtgccgga actccgccgc accgaaccgg accggcccgc cgccggcggc
     5281 tcctggatcg gcctcgagcg ccgtcacgat cacgtggtga cgggtatagc ctcgctgacc
     5341 ctgcttccgg cctgggccgc gctgccgctg atgctgggcc tgcttgtggc tggctggcgc
     5401 cgcgagggaa catagctccc ttctccgtcc ctttgggggc gaagcagctt gcccccaggg
     5461 gatgagtgag ctgggggcct acctaccccc gctgtatcag ctcaatcgag acgttctgcg
     5521 gcgcagtcag gaacgcgatc cggaccccgg gccgtatcgt cttcggctcc atggtgaatg
     5581 ccacaccctt tgccttcagc tctgccaccg ctgcgtcgat atccggcacc gtgagcccga
     5641 agtgttccag ccccatatac ggagtctgcg gcgaggcgcc agtcattgcc gggtctgcct
     5701 tcgcgatgaa gatcttctgc ccggtcaggt ccagatcgat ccgcgtcgat ccatccggct
     5761 gcgacgtcct gatcacctcc gcccccagct tgtccgcgta ccagtccgcc gtcgcagccg
     5821 gatcaggcga gcgcaggtgt atatgttccc atgtatactt catagcttcc tcctacattg
     5881 tcatttccag cacgtacaac ccgccagcga accgatccac cgtatacacc agccggttct
     5941 cgtccacgta aacgtcgttg atcatccccg ccttcatcgg cgatccttcc ggaggctccg
     6001 gaatgaacga cgccacttcc ttcggctgca gcggatcgct gatatcgtgc acccgcagcc
     6061 cgccgccgaa atacgtaccg aacaccagcg tgctggactg gaacgccggc cccggccggt
     6121 tctcccacag gttatgcgcg ccataccgcc caccgagcgc ataatcctcc atcggcggaa
     6181 tcggcagcgt cgccatcgac accagattgt cctcgcgccg cgcgtccagc agccacacca
     6241 gcttcggcca atcggcgcct tccgttcgca cgcactcatc gctcaccaca tacaggtcgc
     6301 gatcgaacag aggcatcacc gtgtgggtga acccggggaa cggcggatgg ggattccagt
     6361 gccccaccag cttcggcgcg ccgatatccg agatatccag cacgcacgcg ccgccatcaa
     6421 tatagccgac atacgcccga tccggccgct ctggatatac attagtattg tgcgcccgcc
     6481 acccggtgtc gaacttcgga tgccggggca ccggcggtgc cgcatcgcgt tcttctgtgc
     6541 cgggatacca ccagcgcccc agctccttgg gcttcgtcgg atccctcacg tcgaacgccg
     6601 tatagcactg gtcgtccttc agattccgcg gcatgaaatc cgccgccccg ctggaacaat
     6661 ggatggtctt gccgtcgatg aaccacagct gatgcacgcc gcgcgaccgc ggtcccgacc
     6721 gatcaatgaa cgcaatgcgc ttcggctctt ccggcgtgga gatatcgaac agctcgatgc
     6781 cggcgggtgt cgccccatgc gtgccggtct ggtacgccac cgccatcacg tcgccgcaga
     6841 tctccagcga attcgatcgc acgttgcgat gcggtagatc ggtctgcacg atcaccttcg
     6901 gcttgcgggg atcggtcacc tccaccgcgg tgaagttctt cggcgcgcat tcatgcgcca
     6961 tccacatgat ccgccgcccg cccttcgcca gctgcaggct catgccctcg ccaacattgc
     7021 cgaagccgct catcaggtct tgcgagatca ggcggaaatt atgggcttcc ggttgtgcca
     7081 tgcgcgtttc tccccgatga acgatttgac gaacacctaa cccactaacc tgaagccaac
     7141 aacggcgaag tgatgatcga aggtgtaggc aagtctgatc cgcgcgcggc gcatgatcgc
     7201 aaagctgctg gcatcgacgg ctgagagctt atgcaggtcc cgccggcgaa aatactccca
     7261 ggattgctgc agatcgcgca actcgcaggc aagaaccttt accgcacgag gcttatatat
     7321 cgcttccttc caagccaaag cgacatcgcg gtttgcattc ctgtccagga atgtgaatgt
     7381 ctcgatgatg actggaattg aggtgtgaag cgccgcgccg acaccttgca atagctccca
     7441 gtgttcgcgc gcttgctcgt gcagcggatc acgcgagagc gccagggcaa tccaagcggc
     7501 gctgtcgacg aaaactgcct ctccctgccg catcacacgt catcgtagat gctgtcgtgt
     7561 tcgattgacg aacgcttcgg ctcgccgtcc catagagcca ccggtccgga cgcgcgagaa
     7621 ctcagcacgg tcttgcggac tgcatcgcgc accagctccg ccacgctgca tccggatcgc
     7681 gccgcaatct cccgcagcgc gtcgagctct tcctggcgga gatagacctg cgttttgacc
     7741 atatgtgtca aattggagat ggagttgacc actgtcaatg caaggcgtca cagtggcgaa
     7801 cccggcagtg ctttcgcgtc ggttgtgcca tgcgcatttc tccccgatgc tgcgcccgat
     7861 gagcgaggca gcgaacactg tgccggtgac gatactcacc ggcttcctcg gcagcggcaa
     7921 gaccacggtg ctgaaccac
//
LOCUS       DATGEM010000376         6038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1119803, whole genome shotgun sequence.
ACCESSION   DATGEM010000376 DATGEM010000000
VERSION     DATGEM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119803"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VKQ27_11930"
     CDS             complement(<1..545)
                     /locus_tag="VKQ27_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89684.1"
                     /translation="MKANTDHIATSHVGSLPRPDSLIAAFHAVETGKPMDEHTLDTTL
                     RNAVHEVVQHQRDIGIDIPGDGEFGKPMSTRVQYGSWWRYSWGRLGGLKTGTEDIYQM
                     APHRSKPGEVVLTSFGDRRDRTTFAEAYRDPDSGITTGPRPPAPVCVGPIKYIGQEAL
                     AADIANFKAALAAAGVSEGFMT"
     gene            686..2548
                     /locus_tag="VKQ27_11935"
     CDS             686..2548
                     /locus_tag="VKQ27_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89685.1"
                     /translation="MLRRAEALLDPTARQPEEPPVDGLWPFYWHYLRQQRWLIFGLFL
                     FGGLAGIVDVTIPAFIGRVVTLVSGRTPEALVREAWPQLLGMAAVLILLRPAVFMCHV
                     TLVNQVVNPGLSNMIRWQNHWHVVRQSWTFFQNDFAGRIANRVIQAGPALRESVVMSF
                     DAAWYIVVYGSSALLLLASIDWRLTPPIILWFTGYGCILWYFVPRLRERSRTVSEMRS
                     LLTGRVVDSYTNILTVKLFARARDEDAFVREAVDLHTGAFRDQTRMITGYIMILALMN
                     AALIVGTSALAIWQWSIGRITVGSVATVLPLTWQITNMAGWVARSVTAIFENIGTVQD
                     GMKSIAVERQMPDPPEARALQVTRGEIRFEDVHFDYGRQRRPDRGGVLHGIDLTIAAG
                     ERVGLVGHSGAGKSTLVNLLLHFYNPERGRILIDGQDIGTVTQESLRTQIAMVTQDTS
                     LLHRSISDNIRYGRPEATDAMIEDAARRAEAHEFIVGLEDWHERRGYDAHVGERGVKL
                     SGGQRQRVALARVILKNAPILVLDEATSALDSEVEAAIQSQLDGLMEGRTVIAIAHRL
                     STIARMDRLVVLDHGRIVEAGTHAQLLARDGTYARLWRRQSGGFTPELMETAAK"
     gene            2609..2863
                     /locus_tag="VKQ27_11940"
     CDS             2609..2863
                     /locus_tag="VKQ27_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89686.1"
                     /translation="MHIALNPLVALGVVLATALTDALYVMFTAAVAARRPIAAANWSA
                     TWYLVSSFAVISYTSNVVYVVFAAIGSWCGAYLSLAWLRR"
     gene            complement(3257..4081)
                     /locus_tag="VKQ27_11945"
     CDS             complement(3257..4081)
                     /locus_tag="VKQ27_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY89687.1"
                     /translation="MLLWSSSFIASRAGLRYLSPLLFVSVRMVIAAAALILVMILLNR
                     SWRVLSGRWGHLAVSGVLTNSLLLMTAHYAMVHTGAAQIALVQTLNPLLTAVVAWPVL
                     GEKLRPLQWLGLLLGATGVVLIVGLAALRSPVELHGLLLTVGGVVGMCSGTLYFGRFC
                     RGIPMIEGTTVQFIAAAIACVAATLLLETPHATWNEIAIASVGWNVVAVSLGGMALYY
                     FMLSHGTAARAAANFYLVPGTASLLAWILLGERLAALTIGGLVVSSLGCWLVARRD"
     gene            4547..5917
                     /locus_tag="VKQ27_11950"
     CDS             4547..5917
                     /locus_tag="VKQ27_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HLY89688.1"
                     /translation="MTARILIVDDVPANTRLLEAKLSAEYYQVASAKDGFEALRMAHE
                     WQPDLILLDVMMPGMDGFECCRKLKEDATTLHIPVVMITALGEPAERLRGLESGADDF
                     LTKPVEYETLMARVRSLVRLKRLLDEWRARGETARAMGLTGDSVAVPTVAGARALVVD
                     DWDLGAQTMQEALSRDGIVAGRARSGSEALALSSANPFDLIVLSLSLIEEDPLKLASS
                     LRAADATHDIPLLLIAEPEERDSILRGFDLGANDWLVLPLDENELRARARNQIRRKFY
                     QDRLRTDLGTALEMALIDPLTGMYNQRYLMRHLRGLLENAQQRELAVLMVDVDHFKSV
                     NDDYGHAAGDRALRAIADALRSNTRVFDSLARYGGEEFVVVMPGTGLDDASLAAERLR
                     VAVEETPFLCDATRTIRLTVSVGVGCSPLHATTPEALLRAADVALYAAKRAGRNRVEI
                     AADTPT"
     gene            complement(5910..>6038)
                     /gene="rpmG"
                     /locus_tag="VKQ27_11955"
     CDS             complement(5910..>6038)
                     /gene="rpmG"
                     /locus_tag="VKQ27_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLY89689.1"
                     /translation="SADTGFFYVTKKNARTQTGKLELKKYDPVVRKHVAFREAKIK"
BASE COUNT         1038 a         1746 c         2112 g         1142 t
ORIGIN      
        1 gtcatgaagc cttccgacac accggcggcc gcgagggctg ccttgaagtt ggcgatgtcg
       61 gcggcgaggg cttcctggcc gatatacttg atgggcccga cgcagactgg ggcggggggg
      121 cgcgggccag tggtgatgcc ggagtcggga tcgcgatagg cctcggcgaa ggtggtacgg
      181 tcgcggcggt cgccgaaact ggtgaggacc acttcgcccg gcttcgaacg gtggggggcc
      241 atctggtaaa tgtcttccgt gccggtcttg agtcccccga gacggcccca ggagtagcgc
      301 caccaggagc catactggac ccgcgtgctc atcggcttgc cgaattcgcc gtcgcctggg
      361 atgtcgatgc cgatatcgcg ctgatgttgc acgacttcat gcacggcatt gcgcagcgtt
      421 gtatcgagtg tgtgttcgtc catcggcttg ccggtttcca cggcgtggaa cgcggcgatc
      481 agggaatcag ggcgcggcag gctgccgaca tggctggtgg cgatgtggtc tgtgttggcc
      541 ttcatggctt cctcccttgt gggtggaggc tacagcgtta tccggctggc tgaaacggtt
      601 tgactgatct ccggggcggc attacccatc ccgccccgtg cggcatgcct gtcgtcgggt
      661 ttccgatgga gttttccccc gcgccatgtt gcgccgcgcc gaagccctgc tcgatccgac
      721 tgcccggcag cccgaagagc cgccggtgga cggactgtgg ccgttctact ggcattattt
      781 gcggcagcaa cgctggctga ttttcggact gttcctgttc ggcggcctcg cgggcatcgt
      841 cgatgtgacg attccagcgt tcatcggacg ggtggtgacc ctggtgtccg gccgcacgcc
      901 cgaggcgctg gtgcgcgagg cgtggccgca gcttctgggg atggcagcgg tgcttatatt
      961 gctgcgcccc gcggtattca tgtgccacgt gacgctggtc aaccaggtgg tgaacccggg
     1021 cctgtcgaac atgatccgat ggcagaacca ttggcatgtg gtgcggcaga gttggacctt
     1081 cttccagaac gatttcgccg ggcggattgc caatcgcgtg atccaggcgg ggccggcact
     1141 gcgcgagagc gtcgtgatgt cgttcgatgc ggcatggtac atcgtcgtct atgggagcag
     1201 cgcgctgctg ctgctggctt cgatagactg gcggctgacg ccgccgatca tcctgtggtt
     1261 caccggatat ggctgcatac tgtggtattt tgtgccgcgg ctgcgcgaac gctcgcgcac
     1321 ggtgtcggag atgcgctcgc tgctcaccgg gcgggtggtc gatagttaca cgaacatcct
     1381 cacggtgaag ctattcgcgc gggcgcggga cgaggacgcg ttcgtgcgcg aggcggtcga
     1441 cctgcacacc ggcgcattcc gcgatcagac gcggatgatc accggctaca tcatgatcct
     1501 cgcgctgatg aatgcggcgc tgatcgtcgg cacctcggcg ctggcgatct ggcagtggag
     1561 catcgggcgg atcaccgtcg ggtcggtggc gacggtgctg ccactgacgt ggcagatcac
     1621 caacatggcg ggctgggtgg cgcgcagcgt gacggcgatc tttgagaata tcggcacggt
     1681 gcaggacggc atgaagtcga tcgcggtgga gcggcagatg cctgatccgc ctgaggcgcg
     1741 cgcattgcag gtgacgcgcg gggaaatccg gttcgaggac gtgcatttcg attatggccg
     1801 ccagcggcgg ccggatcgcg gcggtgtgct gcacgggatc gacctgacca ttgccgccgg
     1861 cgagcgggtg gggctggtcg gtcattcggg tgccggcaag tccacgctgg tgaacctgct
     1921 gctgcatttc tacaacccgg agcgcgggcg catcctgatc gatgggcagg acatcgggac
     1981 ggtgacgcag gagagcctgc ggacgcagat cgcgatggtg acgcaggata cctcgctgct
     2041 gcaccggtca atcagcgaca acatccgata cggcaggccg gaggcgaccg acgcgatgat
     2101 cgaggatgcg gcgcgccgcg cggaggctca cgagttcatc gtggggctgg aagactggca
     2161 cgaacggcgc ggctacgacg cgcatgttgg tgagcgcggg gtgaagctgt cgggcgggca
     2221 gcggcagcgc gtcgccctgg cgagggtgat cctgaagaac gcgccaatac tggtgctgga
     2281 tgaagcgacg tcggcgctgg attccgaggt ggaagcggcg atccagagcc agctcgatgg
     2341 gctgatggaa ggccgcacgg ttatcgccat cgcgcaccgg ctttcgacca ttgcgcggat
     2401 ggaccggctg gtggtgctgg atcacgggcg tatcgtcgag gccgggacgc atgcgcagct
     2461 attggcgcgc gacggaacat atgcgcggct gtggcggcgg cagtcggggg ggttcacgcc
     2521 ggagctgatg gaaacagccg cgaaataacc gtggtgccgg ctggcggcat gggcactacc
     2581 ctgcatgacg atgcattggg ggttcgtcat gcatattgcg cttaatccgc tggtcgctct
     2641 tggcgtggtg ctcgccacgg cgctgaccga cgcgctctac gtcatgttca ccgcagctgt
     2701 cgccgcacgg cggccgatcg ccgcggcgaa ctggagtgca acgtggtatc tcgtgtcatc
     2761 gtttgcggtg atcagctata ccagcaacgt cgtgtacgtg gtgtttgccg cgattggttc
     2821 gtggtgcggg gcgtatctgt cgctggcgtg gctgcgccgc tagatgggga tgagcgctga
     2881 aggacgcgct gatctcgctt tggccctttg tttatcgcgt gattcaaact tccggcgatc
     2941 ccgcctctgg tcatcacggt cgagcgcttt gatgctcaca gatgatgctg cagcaacccg
     3001 tgagggcaag cactggctat ttggccctcg tctggaaatg ccgatcctgc gtgaggatca
     3061 aagtgttcaa gcacaaagtt cgggaaggta cgcgaaggcc accaaggaga cttgttcagg
     3121 gccttcggct tatagagcgg acgaatggcg cacaaaatgt ccttcctgat cttcgtgccc
     3181 ctttgtgaac tttgtgttga attattgtcc caaatgccgg ccttcatagg gaagcaccgc
     3241 cctccatgac ggtgcctcaa tcgcggcgtg caaccagcca gcagccaagc gatgatacga
     3301 caagtccacc aatcgtcagg gccgcaagcc gttcgccgag caatatccag gcgagcagtg
     3361 aggcggtccc cggtaccagg tagaaattcg cagcggcccg ggcagcggtg ccgtgcgaca
     3421 gcatgaagta gtagagcgcc atgccgccga gcgagactgc gacgacgttc cacccaacgg
     3481 acgcaatcgc gatctcgttc catgtcgcgt gaggcgtttc aagcagcagc gtggcggcga
     3541 cgcaagcgat cgcggcggcg atgaactgga cggttgtgcc ctcgatcatg ggtatgccgc
     3601 gacagaagcg gccgaaatac aaggtgccgc tgcacatgcc gacgacaccg ccgacggtaa
     3661 gcagcagacc gtggagttcg accgggctgc ggagcgcggc caggccgacg atgagcacca
     3721 ctccagtggc gccgagcagc aggcccagcc attgcaacgg gcgaagcttc tcgcccagca
     3781 ccggccaggc gaccacggcg gtcagcagcg ggttcagcgt ctgcaccagg gcaatctgcg
     3841 ccgcgcccgt atgcaccatg gcgtaatgcg cggtcatcag cagcaggctg ttggtgagca
     3901 cgcccgatac cgcaaggtgc ccccagcggc ccgacagcac gcgccatgag cggttcagaa
     3961 ggatcatcac gaggatcagc gcggccgcgg cgatgaccat acgcaccgac acgaacaaca
     4021 acggcgacag gtagcgaagc ccggccctgg aggcgatgaa cgaggaactc caaagcagca
     4081 cgaaggcgaa tggggttgat ctcgggccgg gccgcggagc gacgtgttgg cgggctggaa
     4141 ggtgagaggc gtccacggct tcgccttagc gttgcacgca cgcttgcgcc agggtggcgg
     4201 tgtgttagcg cagtataatg gaacctgttt gtaacgtcgg gagcgtcata caggatcagg
     4261 cactgtcctg atcgcgtcaa ctctctctgt attgacagtg cgggatgggc ggcaaatggc
     4321 attgcctgga tggataaccc tcttaggggt ttggcacttt atattcctat attattgcaa
     4381 cgtgtctcta ccctaacaga aggcggcgga tttccgatcg tggcatggtg aacctggcta
     4441 tcggtctgct cactttcgtg tcgcgcggcc atcggttatg gtcgctcgct gttcggtctg
     4501 cgccggcctg gcgtggcctg gtcttccaag gacgattgcg cggccaatga ccgccaggat
     4561 tctgattgtc gatgacgtgc cggcgaatac ccgtctcctg gaggcgaaac tttcggccga
     4621 gtactatcag gtggcaagcg ccaaggacgg cttcgaggcg ctgcgcatgg cgcatgagtg
     4681 gcagcccgac ttgatcctgc tcgacgtgat gatgccaggc atggacggtt tcgagtgctg
     4741 ccggaagctc aaggaagacg ctactacgct tcacattccc gtggtgatga tcaccgcact
     4801 tggggagcct gctgagcggc tgcgtgggct cgaatctggc gccgacgatt tcctgaccaa
     4861 gccggtggaa tacgagacac tgatggcgcg ggtgcgcagc ctggtgcgct tgaagcgcct
     4921 gctggacgag tggcgcgcgc gcggcgagac ggcacgtgcc atgggactga ccggcgattc
     4981 cgtggcggtg ccgacggtcg ccggtgcgcg cgcgctggtg gtggacgact gggacctggg
     5041 cgcgcagacg atgcaggagg cgctgtcgcg tgacggtatc gtggccgggc gcgcccgatc
     5101 gggaagcgaa gcgttggcac tatcgtcggc gaatccgttc gacctgatcg tgctgagcct
     5161 gtcgctgatc gaagaggatc cgctgaagct cgcctcatcc ttgcgcgcgg cggacgcgac
     5221 acatgacatc ccgctgctgc tgatcgccga accagaggag cgtgacagca ttctgcgtgg
     5281 ctttgatctc ggtgcgaacg actggctggt gctgccgctc gatgagaacg agctgcgcgc
     5341 gcgggcgcgg aaccagatac ggcggaagtt ctaccaggac cggctccgga cggatcttgg
     5401 cacggcgctc gagatggcgc tgatcgaccc gctgacgggg atgtacaacc agcgctacct
     5461 gatgcggcat cttcgcgggc tgctggagaa tgcgcagcag cgcgagcttg cagtgctgat
     5521 ggtcgatgtc gaccatttca aatcggtgaa cgacgattat ggccacgcgg ctggcgacag
     5581 ggcattgcgc gcgatcgccg atgcgctgcg ctccaacacg cgggtgttcg attcactggc
     5641 aagatatggc ggcgaggagt ttgtcgtggt gatgccgggc acgggtctgg acgacgcctc
     5701 tttggcggcg gaacggctgc gtgtcgcggt ggaagagacg ccgttcttgt gtgacgcgac
     5761 gcgcaccatc cggctgaccg tgagcgtcgg ggtcggctgc agcccgttgc acgccacgac
     5821 gcccgaggcc ctgctgcgcg cagccgacgt cgcgctttac gcggccaagc gggccggacg
     5881 caatcgcgtg gaaatcgccg ccgacacgcc tacttgatct tggcttcccg gaacgcgacg
     5941 tgcttgcgca cgaccgggtc atatttcttc aactccagct tgccggtctg ggtgcgcgca
     6001 ttcttcttcg tgacatagaa gaagccggtg tcggcgct
//
LOCUS       DATGEM010000377         8617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1259613, whole genome shotgun sequence.
ACCESSION   DATGEM010000377 DATGEM010000000
VERSION     DATGEM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259613"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VKQ27_11960"
     CDS             <1..887
                     /locus_tag="VKQ27_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLY89690.1"
                     /translation="RDLQDSPFDSYIQTDATINHGNSGGPLIDRNGDVLGITTALYNP
                     QENGGFIGIGFAIPSNTASFVENFLLDPNHPKPGWLGVTLQDMNTELSEALDVPQSGG
                     AIISAVAPSGPANQASLHVGDVVETINGTPENDSRAFNRAIVMLKVGEPAHLTIWRNG
                     KQKDVDATAAAWPNYMPYGGMMHGAMAEAMMKRPPDSGMTLAAITDEARKKYGLDPKL
                     IGVLVASVEQDCEARELGIEPGDVIKAVQGEAVASPADIANAMQSAHEQRRPYLAVLV
                     QRNQQLRWLPLSISAAGS"
     gene            complement(996..1439)
                     /locus_tag="VKQ27_11965"
     CDS             complement(996..1439)
                     /locus_tag="VKQ27_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89691.1"
                     /translation="MHLMGKMTLAALLLGTTIVGSALADNSLTTGCPAGTQRLEGEGG
                     AGGNWQPTPGSAVQRTAAASKTEGEGGAGGNWQQAPGQGAQRTAAASKTEGEGGAGGN
                     WQQAPGQGVQRTAAASKTEGEGGAGGNWQPTPGSAVQHTAAAPCK"
     gene            1774..2892
                     /locus_tag="VKQ27_11970"
     CDS             1774..2892
                     /locus_tag="VKQ27_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLY89692.1"
                     /translation="MNEQSTAPEMRVGDSSDAPKVPFLQMPPRRFATAIRLFVLLLIG
                     GLIVFVYYEWDYWIGWATEQTTDDAYLQSDLTPLAAHVPGYAAHLLVHDYEKVKAGQL
                     LVQIDDRDYRAQLAQAQASVEASEAAIATIAQQKLLQNALIAQAKASIEGSKADVTRY
                     HLETIRQQNLLSTRIVGTQQAVEQAVDNERRADATLSLNQAQLQQQEQQINVLTSQEA
                     QARATLETQKAARDLAAINLGYTRITSPVDGMVGLRLVQEGQYLSVGTQVISVVPLPR
                     VWVLANYKETQMTRVRIGQPATVTVDTFPGVVLHGHVESWAPASGAQFSLLPPDNATG
                     NFTKVVQRVTVKILLDPDPKVDDLLRPGMSVIPTIDTK"
     gene            2889..4538
                     /locus_tag="VKQ27_11975"
     CDS             2889..4538
                     /locus_tag="VKQ27_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89693.1"
                     /translation="MSLSHGLAGWLGWHPAAPRDHSLCHPTDKAAQTRPAVSPNPYIG
                     TLAVMIGSVIATLDSRITVFGLADVRGGVHAGFDEGAWITTALTCGQMMIGPVSSWLG
                     AVFGVRRVMEISAAVFAVSNFLLPFSPNVHWVLLFQFISGLSSGTFIPLTIGFVVLSL
                     PPRLVVFGVAAYALNLELSLNVAASIEGWFDDHWNWHWIFWDTALLAPVMLLCIRVGM
                     PRQPVNRELLKTADWSGILYASLGFSILYAALDQGNRLDWLNSGLITALLLGGFLLLV
                     AFVVQELISARPWINLRYAAAGNIPLLFLLITFFRFALLATAYIIPQFLVTVQGYRAV
                     EVGSALLWIALPQFIIAPATGFLLRYVDPRFSLAFGFALVGVGCFMGGQLTHDWVSRD
                     FLAGLLVQALGQSIGLTSLVWFFLRHLEPSEVLTFGAVLQTGRLFGAQLGSAFVQTFV
                     RVREQVASNLLGLHVTPGSYLTHQRLQDYAHAVAGRSVGTPAASARADALLAHAVQNQ
                     ANVLSYIDGFMIIGFATVVVLALMLTLRDPPPSVLPIFRKN"
     gene            4660..7422
                     /locus_tag="VKQ27_11980"
     CDS             4660..7422
                     /locus_tag="VKQ27_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLY89694.1"
                     /translation="MATNTRQPIYVNGDWEIDLSRRELRLRGVPVALGGRAFEIIESL
                     VESAGMLVTKEDLMASVWPGAAIEDNTLQVHISAVRRAFGADRGMLATAFGRGYRLLG
                     DWTVRDDDTPLSQAEPSLPPAPLPRDEITTNLPLAASELIGRGDAVRQLRDLLSIHRV
                     VTLTGPGGIGKTALALETARSLLLGAQSEVWFVELMALADPDLVPTAVAGILRLQPGG
                     GAPSAESVARAIGRRKMLLLLDNCEHLIDAAARLAETVVRACPRASILATTREVLRID
                     GECVLRVPPLDVPALDSEAPTDVLSHSAVHLFIARTRMRNQDFSPRGADMPLIANICR
                     RLEGIPLAIEFAAARAAVLGLYEVATRLDDRFDLLIDGRRTALPRHQTLRATLDWSYD
                     LLPAAEQRILRRLAVFPGGFTLDAAAAVMAPDGSSTVIDGIANLVAKSLVTLDAAASA
                     ERWRLLDTVRAYAQEKLIETGEAEGTARRQAEFFLGLLTDEHADSEIDNIRAALDWAF
                     SPAGNSRLAINLTVACVPLWTRLSLLGECRTRVEAALTVLDGATEPRAELTLRAALGM
                     ALAYTSGPIDAAKPNWQRVRDLADALGDTEYQLRAIYGLCLHETLICEYRVALALALQ
                     FQKIAASASAELPTADRMMATVLHYLGNQAGACEYAERSLAAPLPPDRNLHAAHYGVD
                     QRVGATVQLARALWLRGFPDQAMATAQVAVDEATDVGHANSVCLALADGACIVAILCG
                     DLSAATRFARALTEHADRHALGVWRTYGLALRGRLMIKSQPLEGAALLRSALDGLNDT
                     PFDIRFQLYLVWLAEVLEAAGQASEALTAIDEALERAERTEERWYLPEILRIRGELST
                     DADYLARSFQLAREQNALSWELRAAASRVRGQHAEPALLGDTLARFTEGFSTADLLAA
                     SQLAGE"
     gene            complement(7551..>8617)
                     /locus_tag="VKQ27_11985"
     CDS             complement(7551..>8617)
                     /locus_tag="VKQ27_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLY89695.1"
                     /translation="AMRGNVNVLSIQRISAADRARIEAVDAVIRLTDAGGWFDGEYRD
                     SWPAHSSSRYLTAGAAGRGTREERDALLAEAEVIIGGWPFPLDLRARSPRLKWFHQRP
                     AGASNLRIGDLWGSDVVVTTSRGFANPLPMAEYAIAGILHFAKGLDRAVIDRTDGTFD
                     HWAYRPLLLEGKTLCVVGAGGIGREVGRLGAALGMRVVGTRRSVIADEALPAGFVALG
                     GADALDGFLAEADFVAVCCQWTDETTGLINAPRLALMKPGVVLINVARGEIIDEAALV
                     DALARGHLRGVVLDVYVGEFDRKPPEALWRDPRVLITPHISGASDESRHGGIELFCDN
                     LRAWLDGREMRNVIDWDRGY"
BASE COUNT         1482 a         3069 c         2502 g         1564 t
ORIGIN      
        1 accgcgatct gcaggattcg cccttcgaca gctatatcca gactgatgcc acgataaacc
       61 acggcaattc aggcggcccc ttgatcgatc ggaacggaga cgtcctcggc atcaccaccg
      121 cgctatacaa tccgcaggaa aatggcggct tcattggcat cggctttgcc attccatcga
      181 ataccgccag cttcgtcgag aattttctgc tcgatccgaa ccaccccaaa ccgggatggc
      241 tgggcgtcac cctacaggat atgaacaccg aactgtccga ggcactcgat gtgccgcagt
      301 ccggcggcgc gatcatttcg gcggtcgccc catccggtcc cgccaaccag gcttcgctgc
      361 acgttggcga cgtcgtcgag acgatcaacg gcacgcccga aaacgactcc cgtgccttca
      421 accgggcaat agtcatgttg aaggtcggcg aaccggcgca tctgacaatc tggcgaaatg
      481 gcaagcaaaa ggatgtggat gccactgccg cagcatggcc caattacatg ccgtatggcg
      541 gcatgatgca cggcgcgatg gccgaagcca tgatgaagcg accgcctgat tccggcatga
      601 cccttgccgc cattacggac gaggctcgca agaaatatgg gctcgatccg aagctgatcg
      661 gcgtcctggt tgcgtcggtc gagcaggact gcgaagcacg cgagctcggt atcgaacctg
      721 gcgatgtaat caaggctgta cagggcgagg cggtggcatc gccagccgac attgcaaatg
      781 caatgcaatc agcacacgag cagcgtcgcc cctatcttgc agtgctagtg cagaggaacc
      841 agcaactgcg ttggctgcct ctttcaatta gcgcggcggg atcgtagtac gcgaccggca
      901 cactgacggc agaacacgct cgtatacaca aaaatcccgg ctggcgggtg tgccagacgg
      961 gattttgttt ggcccgctta tcggaagcgg ctgctttact tgcagggcgc cgccgcagtg
     1021 tgctgcacgg cagatccggg cgtcggctgc cagttgccgc cagcaccgcc ctcaccctcg
     1081 gtcttgctcg ccgcggccgt ccgctgcacg ccctgaccag gcgcctgctg ccagttacca
     1141 ccagcgccgc cctcaccttc ggtcttgctc gccgcggccg tccgctgcgc gccctgacca
     1201 ggcgcttgct gccagttgcc accggcaccg ccctcaccct cggtcttgct cgccgcggcc
     1261 gtccgctgca cggccgaccc gggcgtcggc tgccagttgc cgccggcacc accctcaccc
     1321 tcaaggcgct gcgtgccagc cgggcatccg gtcgtcagtg agttgtcagc cagcgcagaa
     1381 ccaacaatcg tcgtgcccag caacagtgct gccagtgtca tctttcccat gagatgcatg
     1441 gtaatcctcc atagggggtt tcccgccgca gccgcaggcc gcttcccgcg aagtcccgcc
     1501 gcttcgtctt caagtctctc gcgcggactt gccgcgccgc tcacgttccg gctttgcgcc
     1561 agtccagccc ggattggatt gaaggtttcg ggcaattcgg cgcaatctgg ccgttcccat
     1621 aacagcaacg cccttcagtc agtcctggtc cgcgcctctt attccatgca tcgtcgcgcg
     1681 gcacggccga ccgcagtttt cagcaagaac gctcggcggc aactcgacac cttcgtttct
     1741 cgccaccact cggtggtccg ggagccgcca cgtatgaacg agcaatcaac cgcacccgag
     1801 atgcgcgtcg gcgacagctc ggatgcgccg aaggtcccgt tcctgcaaat gccaccgcgc
     1861 cggttcgcaa ctgccatccg cttgttcgtg ctgttgctga tcggtggttt gatcgtgttc
     1921 gtctattacg agtgggacta ttggattggc tgggccaccg aacagacaac ggacgacgcc
     1981 taccttcagt ccgatctcac gcccctagcc gcgcacgtcc cgggttacgc ggcgcatttg
     2041 cttgtgcacg actacgagaa ggtgaaagcc ggccagcttc tggtccagat cgacgatcgc
     2101 gactaccgcg cccagctcgc ccaggcacag gccagcgtcg aggcttccga ggcggctatc
     2161 gccacaatcg cccagcaaaa gctcctgcag aacgcactga tcgcccaggc caaggcctcg
     2221 atcgaaggca gcaaggccga cgtgacccgc taccacctcg aaacaatccg gcaacagaac
     2281 ctgctctcca cccgcatcgt cggaacgcag caggcggtcg agcaggctgt cgacaatgaa
     2341 cgccgcgccg acgccacact ctcgctcaac caggcgcaac ttcagcagca ggagcagcag
     2401 atcaacgtcc tgaccagcca ggaggcacag gcgcgcgcca cgctggagac gcagaaggca
     2461 gcacgtgacc tcgcagcgat caatctcggc tacacccgca tcacctcgcc ggttgacggc
     2521 atggtcggcc tccgcctggt gcaggagggc cagtatctga gtgtggggac ccaggtcatc
     2581 tcggtcgtcc cgctgccccg cgtctgggtg cttgcaaatt acaaggaaac ccagatgacc
     2641 cgggtacgca tcggccaacc ggcgaccgtt accgttgaca ccttccccgg cgtcgtgctg
     2701 cacggccacg tcgaaagctg ggctccggcc tccggcgcgc agttctcgct gctgccaccg
     2761 gataatgcga ccggcaattt caccaaggtg gtgcagcgcg tcacggtgaa gatcctgctc
     2821 gatcccgacc caaaggtgga cgacctgctg cgccctggca tgtcggtgat cccgacaatc
     2881 gacacgaaat gagcctgagc cacggtctcg caggctggct cggctggcat ccggccgccc
     2941 cacgggacca ttcgctgtgc catcccaccg acaaggcagc acagacccgt ccagcagtca
     3001 gcccaaaccc ctacatcggc acactggcgg tgatgatcgg ctcggtgatc gccacgctcg
     3061 acagccgtat cactgtgttc ggcctcgccg acgtgcgcgg cggcgtgcat gccggcttcg
     3121 atgaaggcgc atggatcacc accgctctca cctgcggcca gatgatgatt ggcccggtat
     3181 cgtcgtggct tggcgccgtg ttcggcgtac ggcgcgtaat ggagatctcc gctgccgtat
     3241 tcgcggtctc caacttcctg ctgcccttct cgccaaacgt ccattgggtg ctgctgttcc
     3301 agttcatcag cggcctctcg tccggcacct tcatccccct gaccatcggc ttcgtcgtgc
     3361 tgagcctacc gccgcgactg gtggtgttcg gcgtcgccgc ctacgcgctc aatctggaat
     3421 tgtcgttgaa cgtcgccgcc tcgatcgagg gctggttcga cgatcactgg aactggcact
     3481 ggatcttctg ggataccgcg ctgctcgcgc cggtcatgct gctctgcatc cgcgtcggca
     3541 tgccgcgcca gccggtcaat cgagaattac tgaagacggc ggactggtcc ggcatacttt
     3601 atgcctcgct cggcttcagc atcctttatg cagcgctcga tcagggcaac cgcctcgact
     3661 ggctgaattc cggcctgatc acggctctgc tgctgggcgg tttcctcctg ctcgtcgcat
     3721 tcgtggtgca ggaactcatc agcgcacggc cctggatcaa tctccgctat gccgctgcgg
     3781 ggaacattcc cctgctgttc ctgctgatca cgttcttccg cttcgccctg ctcgccaccg
     3841 cctacatcat cccgcagttc ctggtcacgg ttcagggcta tcgtgcggtc gaggtcggca
     3901 gcgccctgct gtggatcgcc ctgccgcagt tcatcatcgc gcccgcgacc ggcttcctgc
     3961 tgcgctatgt cgacccgcgc ttctccctgg ccttcggttt cgctcttgtc ggcgtcgggt
     4021 gcttcatggg cggtcagctc acccatgact gggtcagccg tgattttctc gcagggctgc
     4081 ttgtgcaggc gctgggccag tccatcggcc tcacgtcgct ggtctggttc ttcctcaggc
     4141 acctggaacc gagcgaggtg ctgacattcg gagccgtgtt gcagaccggc cgcctgttcg
     4201 gcgcccagtt gggatctgcc ttcgtgcaga cctttgtccg cgtgcgcgag caggtcgctt
     4261 ccaacctgct cggcctgcac gtcacgccag gttcctacct gacccaccag cggcttcagg
     4321 actatgccca cgcagtggcc ggacggtcag tcggcacccc cgcggcaagc gcccgtgccg
     4381 acgccctgtt ggcccatgcc gtccaaaacc aggcgaacgt tctatcctat atcgacgggt
     4441 tcatgatcat cggcttcgca accgtagtcg tcctggccct gatgctgacg ttgcgggatc
     4501 cgccaccctc cgtgctgccg atcttccgca agaactgatc ccggccccac ctcttccctc
     4561 gattaaattt gcgccttttg gacgattcga tcgtcgcgcg cattgccgcg acggcaattc
     4621 tgctcaattc tgaattttcc tgaatccgag ccaggagcag tggcgaccaa tacgcgacag
     4681 ccgatctacg tgaacggcga ttgggagatt gatctttccc ggcgcgagct gcggttgcgc
     4741 ggcgtccccg tcgcgttagg cgggcgcgcc ttcgagatca tcgagagcct ggtagaatcg
     4801 gccggcatgc tggtcacgaa ggaagacctg atggcgagcg tctggccggg ggccgcgatc
     4861 gaggacaaca cgctccaggt ccacatctcg gccgtgcgtc gcgcgttcgg ggccgatcgc
     4921 ggcatgctgg cgacggcgtt cggccgcggc taccggctgc ttggcgactg gaccgtccgc
     4981 gacgatgaca ccccactcag ccaggccgaa ccttccctgc cacccgcgcc gctcccgcgg
     5041 gatgagataa ccaccaacct ccccctcgcc gcctcagagt tgatcggccg cggcgacgcc
     5101 gtccgccaat tgcgcgatct cctctccatc caccgcgttg tcactcttac cggccctggt
     5161 ggcatcggta aaacagctct ggcgctggaa accgcacgct ccctgttgct gggtgcccaa
     5221 agcgaggtct ggtttgtcga gttgatggcg ttggccgacc cggacctcgt gccaaccgcg
     5281 gtcgccggca tccttcgcct gcagcctggt ggcggagcgc cctcggctga atccgttgcc
     5341 cgcgccatcg gccgccggaa aatgctgttg ctgctcgaca actgcgagca ccttatcgac
     5401 gcagccgcac gcctcgccga aacagtggtc cgtgcctgtc ccagggcctc tatcctggcg
     5461 accacccgag aggtcctgcg catcgacggc gaatgtgtcc tccgcgtccc gccgctcgac
     5521 gtgcccgcac tggacagcga ggcccccacc gacgtgctga gccacagcgc cgtccacctg
     5581 ttcattgccc gcacgcgcat gcgcaatcag gatttctctc cgcgcggcgc ggacatgccg
     5641 ttgatcgcca acatctgccg tcgcctggaa ggcattccac tcgccatcga gttcgccgcc
     5701 gcccgcgccg cggtgcttgg cctttacgaa gtcgccaccc gcctggacga ccgcttcgac
     5761 ctgttgatcg atggccgccg caccgccctc cctcgtcacc agacactgcg cgccacgctc
     5821 gactggagtt acgatcttct gcccgcagcc gagcaacgca tcttgcgccg gctggcggta
     5881 ttccccggcg gcttcaccct cgacgccgcc gccgcggtca tggcgccgga tggcagcagc
     5941 acggtcatcg acgggatcgc caacctcgtc gccaagtcgc tggttacgct ggatgctgcc
     6001 gcatcggccg agcgctggcg gctgctcgat accgtccgcg cctatgccca ggaaaaactc
     6061 atcgagaccg gcgaggccga aggcaccgcc aggcgacagg ctgagttctt cctcggactg
     6121 ctgaccgatg aacatgccga cagcgaaatc gacaatatcc gtgccgccct cgactgggca
     6181 ttttctccgg ccggcaacag caggcttgcc atcaatctta ccgtcgcctg cgtgccattg
     6241 tggacacgcc tgtcgctact gggcgaatgc cgcacacgcg tcgaggcggc gctgaccgtc
     6301 ctggacggtg ccaccgagcc tcgtgccgaa ttgacactgc gggctgccct cggcatggcg
     6361 ctcgcttaca ccagcggacc gatagatgct gccaagccaa actggcagcg agtccgcgat
     6421 ctcgccgacg ctttgggcga caccgaatac cagttgcgcg ccatttacgg actgtgcctt
     6481 cacgaaaccc ttatctgcga ataccgcgtc gcgctggctc tggctctgca gttccagaag
     6541 atcgccgcgt ccgctagcgc cgaactgcct acggcggacc gcatgatggc gacggtgctg
     6601 cactatctcg gtaatcaggc tggagcgtgt gaatacgccg aacgcagcct ggccgcgccc
     6661 ctgccgcccg accgcaatct ccacgccgcg cattatggcg tcgatcaacg ggtcggcgcg
     6721 acggtgcaac ttgcccgtgc cttgtggctg cgcggcttcc ccgaccaggc aatggcaacc
     6781 gcacaggttg cggtcgacga agcgacagac gtcggtcatg ccaactcggt gtgtctggca
     6841 ctcgcagacg gcgcctgcat cgtcgcgatc ctctgcggcg atctcagcgc ggccactcgc
     6901 ttcgcgaggg cactcaccga acacgcggat cgccatgcgc tgggcgtctg gcgcacctac
     6961 gggcttgcgc tgcgcggacg gttgatgatc aagagccaac cgctggaagg tgccgccctg
     7021 ttgcgctccg ctctggacgg actcaacgac acgccattcg acatccggtt ccagctgtat
     7081 ctcgtctggc tcgcggaagt cctggaagcc gccggccagg ccagcgaagc gctgacagcg
     7141 atcgacgaag cgctggaacg cgccgaacgc accgaggagc gttggtatct ccccgagatc
     7201 ctgcgcatcc gcggcgagtt atccacggat gcggactatc tcgcccgctc gtttcagctt
     7261 gcgcgcgagc agaacgcgct atcgtgggaa ttgcgcgctg cagccagccg ggtgcgcgga
     7321 caacacgccg aaccggcact attgggcgac acgctcgcac gtttcaccga gggcttttcc
     7381 accgctgatc tgcttgccgc ctcgcagctg gccggcgagt agtccctgta cccagcgccc
     7441 ctctccgccc ccgggaggag acgttgggtg aagtggggaa cagaccagag agtcagccgt
     7501 atggccggtt gagatacgtc aggcgccgcc agtcggtgac catctcaccg ctagtacccc
     7561 ctgtcccagt caatcacgtt ccgcatctcg cgcccgtcca gccacgcccg cagattgtcg
     7621 cagaacagtt cgatcccccc atgccggctc tcatcactcg cgccagaaat atgcggcgtg
     7681 atcaataccc gtggatcgcg ccaaagcgcc tcgggtggct ttcgatcgaa ctcgcccaca
     7741 tacacatcca gcaccacgcc tcgcagatgc ccgcgcgcca gagcgtccac cagcgcggcc
     7801 tcatcgatga tctcgccgcg cgcgacgttt atcagcacca cacccggctt catcagcgcg
     7861 agccgcggag cgttgatcag ccccgtcgtc tcgtctgtcc actgacagca caccgcgacg
     7921 aaatcagcct ccgccaggaa gccatcgagc gcatcggcgc cacccagcgc gacaaaccca
     7981 gctggcagcg cctcatccgc aatcaccgac cgccgcgtcc cgacaacgcg catccccagc
     8041 gccgcgccca gccgtccgac ctcgcggcca atcccgccag cgcccaccac gcatagtgtc
     8101 ttgccctcca gcagcagcgg ccggtacgcc cagtgatcga atgtgccgtc agttcgatcg
     8161 atcaccgcgc ggtccagccc cttggcaaag tgcaggatcc cggcgatcgc gtattccgcc
     8221 atcggcagcg gattggcgaa accgcgcgac gtcgtcacca ccacatcgct accccagagg
     8281 tcgccgatac gcagattgga cgcgccggcc ggccgctggt gaaaccactt cagccgcggc
     8341 gaccgcgcgc gcagatccag tggaaacggc catcccccga tgatcacctc ggcctcggcc
     8401 aacagcgcat cacgctcctc ccgcgtaccg cggcccgccg cgcccgccgt cagataccgg
     8461 gatgacgaat gcgccggcca gctgtcgcga tactcgccat cgaaccagcc gcccgcatcc
     8521 gtcagtcgga tcaccgcatc caccgcctcg atgcgcgcac gatccgccgc gctgatgcgt
     8581 tgtatgctca gtacattgac attgccgcgc atggccg
//
LOCUS       DATGEM010000378         8548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_243143, whole genome shotgun sequence.
ACCESSION   DATGEM010000378 DATGEM010000000
VERSION     DATGEM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243143"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VKQ27_11990"
     CDS             complement(<1..965)
                     /locus_tag="VKQ27_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89696.1"
                     /translation="MLELLDRQQKLTLNQWKIAIAATLGDMLDFFDFFLIGFVLAFIV
                     GDWHLTYGQSAAILFASGISAPFGSLFWGWMADKIGRRKVMIATVLNFSLATGAMAVT
                     PDGAWIYLAICRFIVGLGVTGLYSVDITLVQEFVPASKRGWLTGLTTTMLPVGVLLGA
                     LLGGFATPYIGWRGLFAVGLLPAALTLYIRAWVPESPHWLMRMGRVEEARRSIAWALQ
                     IDPASIALPAVPPVVRHTRWLELFRYPRSLIAGCLTGLTQTGGVGLTLWMVTLFVMVL
                     RIAPPEASKLAIWVSLAAVAGRFFCSWISDAMGRRASGVLTCLIAA"
     gene            1255..2250
                     /locus_tag="VKQ27_11995"
     CDS             1255..2250
                     /locus_tag="VKQ27_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HLY89697.1"
                     /translation="MNRVVQLLPDSFTAGIIAVVALAVVLPCSGDGARWVAFGSNAAV
                     ALLFFLQGARLSRAAVVSATLHWRLHAIILAATFIVFPLLGLALGPLSGTILPQPLYI
                     GLLFLCCLPSAVQASVVFTSVAGGNVAAALCSASLSSILGIVVTPLLGSVLLQAHGTL
                     SVSGIQSIAVHLLLPFVAGQMVQSRIGGWMQRHERLVRMVDRLSVFLMVYAVISAATI
                     SGAWSRLPASGFLVLVLVDATLLLTMLGLTVFASRSLGLSRQDEIAIVFCGSKKSLVT
                     GIPMASLLFPHVSLGLIVLPLMVFHQLQLMTCATLAQRYALGAARRRPAPVTAAS"
     gene            complement(2303..3112)
                     /locus_tag="VKQ27_12000"
     CDS             complement(2303..3112)
                     /locus_tag="VKQ27_12000"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY89698.1"
                     /translation="MKDNSIWRAVREGRAALGTGLKEFASRGVPWIIDASGFDYCMID
                     HEHGAFDLETIADLAGWFQATNVSAIVRIHKSFSYLIPAILDQGIMGIQVSEVDNVEE
                     ARAIVREAKYPPIGNRGISGQGMHTGYRSHGARHATEYAPWANANIITCVSIESLEGL
                     ENVEAIAAVEGMDMIAYGHSDLSARLGVHLQLEHPKFRAAVRRIADACNVHGKLARGS
                     AETEAQIEEYWQLGCKVLNLPGTDVSTYLDGLKARAGRAHARLKSIGVPTP"
     gene            3275..3658
                     /locus_tag="VKQ27_12005"
     CDS             3275..3658
                     /locus_tag="VKQ27_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89699.1"
                     /translation="MITLVVKDYDEAISFYVEKLGFVLRQDTALSAEKRWVVVSPASG
                     AETAVLLARAALPRQETAVGDQTGGRVFLFLETDNFWRDFAALSEKGVHFPESPRVEP
                     YGVVAVFEDLYGNRWDLIEKKTPSD"
     gene            3785..4216
                     /locus_tag="VKQ27_12010"
     CDS             3785..4216
                     /locus_tag="VKQ27_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLY89700.1"
                     /translation="MERMTTGHFFEDLQVGQSDSVSKTITEADILLYAAVSTDTNPIH
                     IDAEAAKSSIFGERIAHGMLSAGLISAVLGTRLPGPGSLYMRQSLRFAAPVKIGDTVK
                     AAATIIALNPEKKRATLSTICTVGDTVVIDGEAYVQVPSRT"
     gene            4213..5142
                     /locus_tag="VKQ27_12015"
     CDS             4213..5142
                     /locus_tag="VKQ27_12015"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HLY89701.1"
                     /translation="MIRIFHDWRELPADTRGATVALGNFDGVHRGHANVIHAAHSARP
                     QKPLAVLTFEPHPREHFRPDDPPFRLTLSAERADALAALGVTLLFELRFDHEFSLMPA
                     ETFVSEVLHQGVGAKHLVCGPDFAFGRRRGGGADFLAQRAEALGMGLTLVPLLADAAG
                     PISATRIRRVLQDGYPERATADLGRPWTIRGTVAHGDARGRTIGFPTANIALGRHLEP
                     ARGVYAVTIRLPDGSSRKGVANIGRRPTVNEGPESRLEVNIFDFSGDIYDAELAVSLH
                     AYIREERKFAGLDALKAQIATDAAEARRLLADT"
     gene            5207..5575
                     /locus_tag="VKQ27_12020"
     CDS             5207..5575
                     /locus_tag="VKQ27_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89702.1"
                     /translation="MATKRLLGVEFLREGLRAREPERPAPGRGVLAGEDIARRRAVLT
                     YSPDILRTLRDMNEPDGVSPRTLFTALRDRIQGKADFGDFQLALDDAYENRYVEIVGK
                     DDFGDAKYRLTDLARSIAPA"
     gene            5647..6420
                     /locus_tag="VKQ27_12025"
     CDS             5647..6420
                     /locus_tag="VKQ27_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89703.1"
                     /translation="MFSILIALVAVLTLVLLRRPAINIQTYFPDTGFGVWLWTFFIGA
                     LVGSAELISRYRDNPGAALSSRPGLLFIALNGIASILALYLMRHFKLAGVDPSKPDQD
                     LVLQALLAGTGAMVVIRSKLFTIRQPGGNDVSFGPAFAVDTFLSSINREVDRRRVPDR
                     NRRVAQRAHALRRCSFKEAAPFLTSALAAFQDMDEDDAKRLRDEFISLATDEAYAAYT
                     DEVKFYLVGFDILTVFGEKAFDSLFDSLTDYFATGEPPH"
     gene            6554..7462
                     /locus_tag="VKQ27_12030"
     CDS             6554..7462
                     /locus_tag="VKQ27_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY89704.1"
                     /translation="MRTNFRWAGLAGAIAMTLAAAPAATAANAPDCPNGGVVRFGVEP
                     YDTSAKLVPIYDHIGKALADKIGCQVQIFVTTTYNAEIEAMRVGKLEIGEFGPLGYVL
                     AHQVAKAEAVAAFGNKEGKPDSYWASLVTWPGSGIKTVAEIRGHSFAFSDPASTSGHL
                     FPAYGLRKAGIDPDKDIRAIYAGSHTSSYEALYNHKVDAGELNSEQVESATQRGHYKD
                     GDVVFLWKSDPIPTDPITVRGDLSPEFKARLTKALQTLDLTVLPDADRKIMGLSGDHF
                     VPQTDGAYDGIRDLVKTLNIDLNKLS"
     gene            7462..8205
                     /gene="phnC"
                     /locus_tag="VKQ27_12035"
     CDS             7462..8205
                     /gene="phnC"
                     /locus_tag="VKQ27_12035"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HLY89705.1"
                     /translation="MLRVGRLTLRYPTGKQALAGVDLDVSAGELLIVLGGNGCGKTTL
                     LRCITRALAPTEGEVWLNGHEISRLSGERLRRARLDVAMVWQHASLVRRRSVLANVTS
                     GALGRNQTWRTAFGGLPPDEVDLARRYLHQVGLPHLAQQRAGTLSGGQAQRVAIARAL
                     AQRPVVMLADEPVASLDPEAAQEIMRLLRKMAHDEGLAVLCVLHQVDLAFAYADRVVG
                     MRDGRVAFDRRRADVSHDQVQRLYLAEAA"
     gene            8202..>8548
                     /locus_tag="VKQ27_12040"
     CDS             8202..>8548
                     /locus_tag="VKQ27_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89706.1"
                     /translation="MSATVAPSGWRNAAAVALRTVAVVVAILIVVQTLIVVQARPQDL
                     ITGFHGMVDIVRRASPPDFAKLPDVVWPTLETIDIAIFGTIGGVLMALPLAVMAATRH
                     GGQRLADCRRTRST"
BASE COUNT         1475 a         2887 c         2659 g         1527 t
ORIGIN      
        1 gcggcgatca ggcaggtaag aacgcccgac gcacggcggc ccatcgcgtc ggagatccac
       61 gagcagaaga agcgaccggc caccgcggcg aggctgaccc agattgccag cttcgatgcc
      121 tcgggtggcg ctatccgcag aaccatgacg aacagcgtga ccatccaaag agtgagtcca
      181 acgccgccgg tctgagtgag gccggtgagg catcccgcga taagactgcg gggatacctg
      241 aacagctcca gccagcgggt atgcctgacg acaggtggca cagccggcag ggcgatcgaa
      301 gctggatcga tctgcaatgc ccatgcgatc gagcgacggg cctcctcgac ccgccccatg
      361 cgcatcagcc aatgcggcga ctccggtacc cacgcgcgga tatacaaagt gagcgcggcc
      421 ggcagaaggc caacggcaaa aaggccacgc caaccaatat agggggtcgc gaaaccgccc
      481 agcaacgctc ccagcaatac gccgacaggc agcatggtcg tggtcaggcc ggtcagccag
      541 ccacgcttcg acgccggcac gaattcctgc accagcgtga tatcgacgga gtagagtcca
      601 gtcacgccca ggccaacgat gaaacgacat atcgcgaggt agatccaagc gccgtctggc
      661 gtcacggcca tcgcgccggt ggcgagggaa aagttcagca ccgtggcgat catgaccttg
      721 cggcggccga tcttgtccgc catccagccc cagaacagcg agccgaacgg tgcactgatg
      781 cccgatgcga acagaatagc cgccgactga ccataggtca ggtgccagtc accaacgatg
      841 aaggccagta cgaacccgat caggaagaaa tcgaaaaagt ccagcatgtc gccgagagtt
      901 gcggcgatcg cgatcttcca ttggttcaga gtgagcttct gctgccgatc gagcagttcg
      961 agcatgacgg cacctcccct gtgggcgaca cgagcggccg gccttgctgt tgattacggg
     1021 cgtgtctctt gacggccggt caatcacatt gcggcaggcg ccatcaccaa tgcgtcgggg
     1081 gatagcagcc tcgttgcgcc catcatctga cttgctgggc gagcgtcgac tcacatccgc
     1141 gtgagaaggg cttgcctgcc ggtcgggcaa ccgcttgctc gacgaccgcc gcgtcgccta
     1201 acgtctcggg accgaatgat tccgaggtag acgggaggcg gcggggaaac agcattgaac
     1261 agggtcgtgc agctgctccc cgacagtttt accgccggga tcatcgcggt tgtcgcgctc
     1321 gccgtggtgt tgccctgcag cggagatggc gctcggtggg ttgcgttcgg cagcaatgcc
     1381 gctgttgcgc tgttgttttt cctgcagggc gcgcggcttt cccgggcagc ggtggtcagt
     1441 gccacgctgc attggcggct gcacgcgatt atcctggctg caaccttcat cgtgtttccg
     1501 ctgctcggtc tggcgcttgg cccgctctcg ggaacgatac tgccgcagcc gctctatatc
     1561 ggcctgctgt tcctttgctg cctgccgtcg gctgtgcagg catccgtggt gttcacctcg
     1621 gtcgcgggcg gcaacgtagc ggcagcgctc tgcagcgcat cgctttcaag cattcttggt
     1681 attgtcgtga cgccgctgtt gggcagcgtg ctgctccagg ctcatggcac cctgtcggta
     1741 agcggcatcc aatcgatcgc ggtgcatctt ttgctgcctt tcgtcgccgg acagatggtg
     1801 cagagcagaa tcggcggatg gatgcagcgg cacgagaggc tggttcgcat ggtcgaccgc
     1861 ctttcggtat tcctgatggt ttatgcggtg atcagcgctg cgacgatatc cggtgcgtgg
     1921 tctcgcctgc cggcatcggg tttcctcgtc ctggtgctcg tcgacgcgac gctgttgctg
     1981 acgatgctgg ggctgaccgt ttttgcctcg cgcagccttg gtctttcgcg gcaggatgaa
     2041 atcgcgatcg tgttctgtgg gtcgaagaag agcctggtaa ccgggatccc gatggccagc
     2101 ctgctgttcc cccacgtctc tctcggtctc atcgtgctgc cgctgatggt gttccaccag
     2161 ttgcagttga tgacatgcgc cacgctcgcc cagcgctatg cactgggtgc cgcacggcgc
     2221 aggccggcgc ccgttaccgc ggcatcgtag ccgcaggccg cggcacgctt atcgggtttc
     2281 ggttctcaca ccggcgcgtt gctcaaggcg tcggcacgcc gatggatttg agccgcgcat
     2341 gggctcgccc ggcgcgcgcc ttcagtccat cgagataggt gctgacatcg gtgccgggca
     2401 ggttcagaac cttgcagcca agctgccagt actcctcgat ttgcgcctcg gtctccgccg
     2461 acccgcgggc aagcttgcca tgcacgttgc aggcgtcggc gatgcggcgc acggctgccc
     2521 tgaacttcgg atgctcgagc tggagatgca cgccaagccg cgcactgagg tccgaatgac
     2581 cataggcgat catgtccata ccctccaccg ccgcgatcgc ctcgacattc tccaggccct
     2641 cgagcgactc gatcgatacg caggtaatga tgttggcatt ggcccatggc gcatactcgg
     2701 tggcatgacg cgcgccatgg ctgcggtagc cggtgtgcat cccctgcccc gagataccgc
     2761 ggttgccgat tggcggatac tttgcctccc gcacaatagc gcgtgcctcc tcgacgttat
     2821 cgacctcgga gacctggata cccatgattc cctgatcgag gatcgcgggg atcaggtagc
     2881 tgaaggactt gtggatacgc acgatcgccg agacattggt cgcctgaaac cacccggcga
     2941 gatccgcgat cgtttcaagg tcgaacgcgc catgttcatg atcgatcatg cagtagtcga
     3001 agccggaggc atcgatgatc cacggcacgc cacgggacgc gaactccttg agtccggtgc
     3061 cgagcgcggc ccgaccctcg cgcaccgccc gccatatgct gttgtctttc atggatcgtc
     3121 caaccctccc gtcacgctct ggcagcatac gccaccagcg atggcgccag aacccgcacc
     3181 ttccccgcgc cggtggggaa caactacggg agcgcggttg ctggacatgt gggggtccgg
     3241 atgctctggt ccggcaatgc ccaggcggat cggcttgata actctcgtcg tgaaggatta
     3301 cgacgaagcg atctcgtttt acgtcgagaa gctcggcttc gtgttgcggc aggacacggc
     3361 cctgtcggcc gaaaagcggt gggtcgtggt atcccctgca agcggcgccg aaacagcagt
     3421 gcttcttgct cgcgcagcac taccgcgcca ggagaccgcg gtaggagacc agaccggagg
     3481 gcgggtcttt ctgttcctgg agaccgacaa cttctggcgt gatttcgctg ctctctccga
     3541 gaagggagtg catttcccgg agagtccgcg ggtcgagcct tatggtgttg tcgccgtatt
     3601 cgaggacctt tacggcaatc gctgggacct catcgagaag aagacgccca gcgattgacg
     3661 gccagccccg gacccgaaac cggtcatcgg gcccggccgc cgacgtgaca tccccttccc
     3721 gccaccgcgc ccatgcgatt gatttgcccg tcacattccc ccagatagcg cccacacacg
     3781 ccggatggag cgcatgacca cagggcactt cttcgaggat ctccaggtcg gccagtccga
     3841 cagcgtcagc aagacgatca cggaagccga tatcctgctg tatgccgccg tctccaccga
     3901 caccaaccca atccatatcg acgccgaggc cgccaaatcc tccattttcg gtgagcggat
     3961 cgcccatggc atgctctccg ccgggctgat cagcgcggtg cttggcaccc gcctccctgg
     4021 ccccggctcg ctctacatgc gccagtcgct gcgcttcgcc gccccggtga agatcggtga
     4081 tacggtaaaa gccgccgcca ccatcatcgc cctgaacccc gaaaagaagc gggccacgct
     4141 gtccactatt tgcaccgtcg gcgacacggt ggtgatcgac ggcgaggcct acgttcaggt
     4201 cccatcccgc acgtgatccg catcttccac gactggcgcg agcttccagc cgacacccgc
     4261 ggagccactg tcgccctcgg caacttcgat ggcgtccatc gcggccacgc caacgtcatc
     4321 cacgccgccc attcggcccg cccgcaaaag ccgcttgcgg tgctcacctt cgagccgcac
     4381 ccccgcgagc acttccgccc cgacgacccg ccgtttcggc tcaccctctc cgctgaacgc
     4441 gccgacgcgc tggccgccct cggcgtcacc ctgctgttcg agcttcgctt cgaccacgaa
     4501 ttcagcctta tgccggctga gactttcgtc tccgaggtcc tccaccaggg ggtgggcgcg
     4561 aagcacctgg tctgcggacc tgacttcgcg ttcggccgcc gtcgtggcgg cggcgccgac
     4621 ttcctcgccc agcgcgccga ggccctcggc atgggactca ccctcgtgcc gctgctcgcg
     4681 gatgccgccg gcccgatctc ggccacccgt atccgccgcg tcctccagga cggctacccg
     4741 gagcgcgcca ccgcagatct cggccgcccc tggacgatcc gtggcacggt ggcccacggc
     4801 gatgcgcgag gccgaaccat cggcttcccc accgccaaca tcgcgctagg ccgccacctg
     4861 gagccggccc gcggcgtcta cgcggtgacc atccgcctgc ctgacggctc ttcccgcaag
     4921 ggcgtcgcca acatcggccg ccgtcccacc gtgaacgaag gcccggagag ccgcctcgag
     4981 gtcaacatct tcgacttcag cggcgacatc tacgacgcgg aacttgcggt ctcgctgcac
     5041 gcctacatac gcgaggaacg gaaattcgcc ggcctcgatg cgttgaaggc gcagatcgcc
     5101 accgacgcgg cggaagcacg caggctactg gccgacacat agccgacgac gtccttggca
     5161 ggatacggtc caggtacgat atcctgcact gaaggaggcg ttgatcatgg cgacgaaacg
     5221 gttgctgggc gtcgagttcc tgcgagaagg cttgagggcc agagagccgg agcgtcccgc
     5281 gccaggccgc ggcgtcctcg ccggagagga tatcgcgcgc cggcgcgccg tcctgaccta
     5341 tagtcccgac atactgcgca ccttgcgcga catgaacgag ccggacggtg tttcgccgcg
     5401 cacgctgttc accgcgctgc gtgaccgcat ccagggcaag gccgatttcg gcgacttcca
     5461 gctcgcgctc gatgacgcgt atgaaaaccg ctatgtcgag atcgtcggca aggatgattt
     5521 cggcgatgcg aaataccggc tgaccgacct cgccaggtcg atcgcacccg cctgacctga
     5581 tccgtgcagc ctccgaggcc aacaccgcca gattctggcc gcggcgccag cgcggattgc
     5641 atcgtattgt tttcgatcct tatcgctctc gtagcagtgc tgaccctggt cctgctccga
     5701 cggcccgcta tcaacatcca gacatatttt cccgataccg gcttcggcgt ctggctctgg
     5761 acgttcttca tcggcgcgct ggttggctcc gcagaactga tctcccgcta tcgcgacaac
     5821 ccgggcgccg ccctgtccag ccggcccggt ttgctgttca tcgcgttgaa tggcatcgcg
     5881 tcgatcctcg cgctctacct gatgcgccat ttcaagcttg ccggcgtcga cccgagcaag
     5941 cccgaccagg acctcgttct ccaggctctt ctggccggca ccggtgcgat ggttgtgatc
     6001 cgctcgaagc tgttcaccat ccgccagccc ggcggcaacg atgtgtcctt cgggcccgcc
     6061 ttcgcggtgg acacgttcct gtccagcatc aatcgcgaag ttgaccgccg ccgcgtaccg
     6121 gaccgcaacc ggcgtgtcgc gcagcgggcg cacgcattgc ggcgctgtag cttcaaggag
     6181 gcggccccgt ttctcacctc cgcgcttgcc gccttccagg acatggacga ggacgatgcg
     6241 aagcgactgc gggacgaatt catctcgctg gcaactgacg aggcctacgc tgcctacacg
     6301 gacgaggtga aattctacct ggttggcttc gacatcctca ccgtgttcgg cgagaaggcg
     6361 ttcgacagcc tgttcgactc actgaccgac tatttcgcca cgggcgagcc gccgcactag
     6421 cgcccgctct ccaatccccc ggacctgttc gcaggtcatc cgggtccgac acggaacctt
     6481 catcgatccg tcatcgctcc tccatccacg gcgcctagac catccccaca gctgcaggaa
     6541 tggggagaca tcaatgcgaa cgaatttccg ctgggccggc ctggccggcg ccattgccat
     6601 gacgctggcc gccgcacccg ccgcgacagc cgccaatgcg ccggactgcc cgaacggcgg
     6661 cgtggtccgc ttcggtgtcg agccatacga cacgtcggcc aagctcgtcc ccatctacga
     6721 ccacatcggc aaggcgctgg ccgacaagat cggctgccag gtgcagatct tcgtcaccac
     6781 cacctacaac gccgagatcg aggcaatgcg tgtcggcaag ctcgagatcg gcgagttcgg
     6841 tccgctcggc tacgtgctgg cgcatcaggt ggcgaaggcc gaggccgttg ctgctttcgg
     6901 caacaaggaa ggcaagccgg acagctattg ggcgagcctg gtgacctggc ccggttccgg
     6961 catcaagacg gtggcggaga tccgcggcca ctccttcgcg ttctccgacc ctgcatccac
     7021 ctcgggccac ctgttcccgg cctatggcct gcgcaaggct ggcatcgatc cagacaagga
     7081 catccgcgcg atctacgccg gcagccacac ttcgtcatat gaggcgctgt acaaccacaa
     7141 agtcgatgct ggcgaactga acagcgagca ggtggagtcg gccacgcagc gcggccatta
     7201 caaggatggc gatgtggtct tcctctggaa gtccgatccg atcccgaccg acccgatcac
     7261 cgtgcgcggc gacctctcac ccgaattcaa ggcacggctg accaaggcgc tgcagacact
     7321 cgacctgacg gtattgcctg atgccgacag gaagatcatg ggcctgagcg gcgaccactt
     7381 cgtacctcag accgacggcg catatgacgg catccgcgat cttgtgaaga cgctcaacat
     7441 cgacctgaac aagctgagct gatgctgcga gttggcaggc tgacgttgcg ctatcccacc
     7501 ggcaagcagg cgcttgccgg tgtcgatctg gacgtctcgg cgggcgagtt actgatcgtg
     7561 ctcggcggca atggctgcgg caagacgacg ctgctccgct gcatcacccg cgcgttggca
     7621 ccgaccgaag gcgaggtctg gctgaacggg cacgaaatct cgcgcctctc cggggagagg
     7681 ctgcggcgtg cgcggctcga cgtggcgatg gtctggcagc acgcgagcct ggtgcgccgt
     7741 cgcagcgtgc tggccaacgt cacgtccggt gcgttgggcc gcaaccagac atggcggaca
     7801 gcgtttggcg gattgccgcc ggacgaggtc gacctggcgc ggcgttatct gcatcaggtc
     7861 ggcctgccgc acctggcgca gcaacgcgcc ggcacgttgt ccggcggcca ggcgcagcgc
     7921 gtggcgatcg cccgcgccct ggcccagcgg ccggtggtca tgctggccga cgaaccggtc
     7981 gccagtctcg accccgaagc cgcgcaggag atcatgcgcc tgctgcgcaa gatggcgcac
     8041 gacgagggcc tcgcggtgct gtgcgtactg caccaggtgg atctggcatt cgcctatgcg
     8101 gaccgcgtgg tcggcatgcg cgacggtcgc gtcgccttcg accgccgccg ggccgacgtg
     8161 tcgcacgatc aggtccagcg cctgtatttg gccgaggccg cgtgagcgcc accgttgcgc
     8221 ccagcggctg gcgcaacgcc gccgccgtgg cgctgcgcac cgttgcggtc gtggtggcga
     8281 tcctgatcgt cgtgcagaca ctgatcgtgg tgcaggcgcg gccacaggac ctgatcaccg
     8341 gcttccacgg catggtagat atcgtccgcc gcgcctcacc tcccgatttc gccaagttgc
     8401 cggacgtggt ctggccgaca ctggagacga tcgacatcgc aatcttcggc actatcggcg
     8461 gtgtgctgat ggcgctgccg ctcgcggtga tggccgcaac cagacatggc ggacagcgtt
     8521 tggcggattg ccgccggacg aggtcgac
//
LOCUS       DATGEM010000379        10037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_246024, whole genome shotgun sequence.
ACCESSION   DATGEM010000379 DATGEM010000000
VERSION     DATGEM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246024"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2477
                     /gene="secA"
                     /locus_tag="VKQ27_12045"
     CDS             <1..2477
                     /gene="secA"
                     /locus_tag="VKQ27_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HLY89707.1"
                     /translation="VQLVGGMVLHDGKISEMKTGEGKTLVATLPVYLNALAGKGVHVV
                     TVNDYLASRDADWMGQIYHFLGMEVGVIVHGQDDEKKRMQYAADITYGTNNEFGFDYL
                     RDNMKYRLEDMVQREFFFGIVDEVDSILIDEARTPLIISGPAEDSSDLYRQVDAVVRE
                     LVKDPSTYDKDEKMRTVALTEPGSERVEDMLREAGVLTEGNLYDIFNVSVVHHVQQSL
                     RAHTLFTRDVDYIERQGKIVIIDEFTGRMMEGRRYSEGLHQALEAKEHVEVQAENQTL
                     ASITFQNYFRLYPKLGGMTGTAMTEADELAEIYKLDVVEIPTNVPCIRNDEDDEVYRT
                     AAEKYEAVAELIDEARKKGQPVLVGTTSIEKSEVIHEVLRKKRIPHAVLNARFHEQEA
                     EIVAQAGAPGAVTIATNMAGRGTDIKLGGNLEMRLKKELDGIDDPAIHAARAAEINDE
                     IAAAHQKVKEAGGLFVIGTERHESRRIDNQLRGRSGRQGDPGRSRFFLSLEDDLMRIF
                     GSDRMGGMLQRLGLKEGEAIVHPWINKALEKAQKKVEARNFDTRKNVLKYDDVMNSQR
                     KEVYAQRKEFMKVSDVSETVAEMRAEVIDNLVSRRIPEKGFHENWELKELTEDIQRVL
                     NQTLPIEDWGREEGIDETHLRERIEQAADAMMAAKAANLGPELMRFIEKSLLIQVMDA
                     VWKEHLYALDHMRQGIGLRAYGQRDPLNEYKSEAFALFNAMLDELKERVTMMLARVEL
                     APDPVQPPPPTIMQELHPQPAPSFGGEPDAAMEGAGAMTITAPQPLRSEAVDPNDPAT
                     WRNASRNAPCPCGSGKKYKHCHGRLA"
     gene            2667..3335
                     /locus_tag="VKQ27_12050"
     CDS             2667..3335
                     /locus_tag="VKQ27_12050"
                     /inference="COORDINATES: protein motif:HMM:NF037384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LirA/MavJ family T4SS effector"
                     /protein_id="HLY89708.1"
                     /translation="MPEPQWHTLPINLTLAFPLVPHIERYQRIYDYLNTRDEVMLELS
                     AIDAATSSKQTFRAFLERWERQLGFSADVKTIHGIGTKEKIAKHFNSVPDRINYVDNE
                     NLIGEAAFMQILSAKRPMVDVGAGSEHGLLTHRVQWVMIGMWDRRTRSLGVGEYLAPI
                     YQGMANPNARAKQLVRVVDGPNERRNLWDIVVDSFNKNATHPEYLHDRFVKNVFPSLY
                     DKWD"
     gene            3369..4511
                     /locus_tag="VKQ27_12055"
     CDS             3369..4511
                     /locus_tag="VKQ27_12055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY89709.1"
                     /translation="MPSLLAVSTLPPWPVLNGYSLRVAHLLEHLAAQWSITLIAPPVE
                     ALPPAIARHVPVALPPSGVTYPWRFDQTSLRATLNHVVAETHFDRALIFPGAEALWFN
                     RIDLPPAVIDIIDCNPLEFWRGFLSYRGLRQRYRAFREIAIATRYARRMVRSYTATTC
                     VGEADTRWLRRIGGRDTVHMIPNGVEVPIHLTEESATPTLIFTGALDYQPNIEAVLFA
                     AQSIWPRIVAAKPDARFVIAGRNPVPAIRALSNVEIAANVPDMTKVLGESWVAMAPMR
                     SGVGIKNKVLEAWACARPVVMSTLATNGLIVPPDHAALVGTTADAMAGAVLRLFDDAD
                     TRHQLGKSARENVQQHFTWARASARLNKLLCTSCGETCPGLDPGVR"
     gene            complement(4541..5116)
                     /locus_tag="VKQ27_12060"
     CDS             complement(4541..5116)
                     /locus_tag="VKQ27_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89710.1"
                     /translation="MSRLVALMLLMIAAAPMAHADDVTDQINEALKAYQKHDTQTALA
                     ALDAAENLLRQARADTLKDLLPPVPAGWTADDAETTTVGVAMMGGGTTVSRTYHLEAQ
                     QVEVQIMADSPMLQGLATLLGSPFAAAGGMKTVVIGGRRMSYSENDNSYMALIGDKVI
                     VKVEGNKDTPDPTLKSFVGAIDFAGIEKLVR"
     gene            complement(5120..7498)
                     /locus_tag="VKQ27_12065"
     CDS             complement(5120..7498)
                     /locus_tag="VKQ27_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HLY89711.1"
                     /translation="MDTPQTVLSPSARDADRLHVLRQLERKLLWLSSWMIHNANHIRP
                     NRDGLKVGGHQASCASVISIMTALYFEIARPQDRIAVKPHAGPVFHAINYLFGRQTLE
                     KMQGLRQLGGVQSYPSRVKDGPEVDFSTGSVGLGVAMTTFSALMADYVRLHNLGGADV
                     PVGRHIAIAGDAELDEGNIYEALLEGWKHDVRNLWWIVDYNRQSLDSVVPDRLFGRIE
                     GLFRDMGWNVVTIKYGRKLEAAFERDGGEALRAWIDDCPNSLYSALTFQGGASWRQAL
                     LADLGRSKARAIIDPMSDDELGALMTNLGGHDIETVIDTLRAEADGDQPTCFIAYTIK
                     GMGLPFAGHKDNHAGLMTKEQMEVFRASMDIRPGHEWDMFEGLGVPAEELRAFIDACP
                     FATPLTPQGRTLTAPMVAVPAALPMPQVAARKMSTQAGFGDILAEIGRGQGELKALAE
                     HIVTTSPDVTVSTNLGPWVNRRGIFDRHTRNDVFRDAKLASAQRWGMSPKGQHVELGI
                     AEQNLFLLLGAAGLTPAMTGARLLPVGTVYDPFVNRGLDALIYACYQDARFLLVGTPS
                     GLTLAPEGGQHQSINTPLIGIAADRLASFEPAHVDELAILLRHAFEFMQRPDGSAVWL
                     RLSTRQLEQPARSLDAAAVIEGAHWVVPPADGARIAIGYQGPVAPEAAAAFAELVAEE
                     PGAGLFAITSADRLHAGWLAAMRGRRGGDRGAMSHIERLLAPMAPGAALVTVLDGHPA
                     THAWLGSVRGQRVVPLGPDHFGQTGDIPDLYREYGMDADAILDACAQALLAV"
     gene            complement(7557..8084)
                     /locus_tag="VKQ27_12070"
     CDS             complement(7557..8084)
                     /locus_tag="VKQ27_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89712.1"
                     /translation="MADAGADSQVSNAQYRFLFQLNEGGGKVAYDNDLPVPAMLACAA
                     AESGWGGSKIFGKTGCHFNLQKPSWYTWMKCRTVKMQTDGQGDGNLVWVDFCAADDAA
                     ESVGLWCTWILNYPNVGHRNEVLSFRHSPRDFCARLPKVGFGPQNVKAWEETKKGYLD
                     VYDQFQLGSFVWSAP"
     gene            complement(8192..9571)
                     /locus_tag="VKQ27_12075"
     CDS             complement(8192..9571)
                     /locus_tag="VKQ27_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HLY89713.1"
                     /translation="MSDAAAPSLLDRAIRRITTVWRDMASGPVDEPIAAQMRACLDGR
                     GGEVSARNRAAKLAETYQELDDAGRKDFLRTLAAFDSDAEAVGRAYAEVQAAADPAEL
                     AVAKADLRRALEPPRLKLLTQFTTIPDGRKFLVDLREFLLRSRKDDRLLAALEADLRG
                     LLAAWFDIGFLELQRIDWNSPASLLEKLVDYEAVHAIRSWRDIKNRLDSDRRCYAFFH
                     PRMPGEPLIFVEVALVKGLAGSVQSLLDEKAPVQDAREADTAIFYSISNCQRGLAGIS
                     FGNFLIKRVVEELSAEFRNLKAFATLSPIPGFRKWLDAQLASDETDVLSDEEAASLGS
                     AMPAPSGKESLAAIVAKRGWWREVALRKTAEPVLVRLCARYLMMETNGEKRARDPVAH
                     FHLSNGARVERINMLGDTSEKGAKESATLMVNYLYDPAKIEDWHEDYAGEGKRNASTT
                     VRRLARGWS"
     gene            complement(9599..>10037)
                     /locus_tag="VKQ27_12080"
     CDS             complement(9599..>10037)
                     /locus_tag="VKQ27_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HLY89714.1"
                     /translation="FAGEPDALRGAAVMTPHAGEFARAFGAAGADRIDAVRAAAARTG
                     GVVLLKGADTIVAAPDGRVAINASAPPWLATAGSGDVLSGVIGGLLAQGMDAWEAAAA
                     GAWVHGRAGALAGQGLVAEDLLSAIAPALVDASCAPHMSGERL"
BASE COUNT         1999 a         3685 c         2743 g         1610 t
ORIGIN      
        1 acgtgcagct cgtcggcggc atggtcctgc acgacggcaa gatctccgaa atgaagaccg
       61 gcgaaggcaa gaccctggtg gccaccctcc ccgtgtacct gaatgcgctc gccggcaagg
      121 gcgtgcacgt cgtcaccgtc aacgactacc tcgccagccg cgacgccgac tggatgggcc
      181 agatctacca cttcctcggc atggaggtgg gcgtcatcgt ccacggccag gacgacgaga
      241 agaagcgcat gcaatatgcc gccgacatca cctacggcac caacaacgag ttcggcttcg
      301 actacctccg cgacaacatg aagtatcgcc tggaggacat ggtccagcgc gaattcttct
      361 tcggcatcgt cgacgaggtg gacagcatcc tgatcgacga ggcgcgcacg ccgctgatca
      421 tctccggtcc cgccgaggac agttccgatc tctaccgaca ggtggatgcc gtcgtgcggg
      481 aactggtgaa ggaccccagc acctacgaca aggacgagaa gatgcgcacc gtcgcgctca
      541 ccgagccagg ctcggagcgc gtcgaggaca tgctgcgcga ggccggcgtg ctgaccgaag
      601 gcaacctcta cgacattttc aacgtctcgg tcgtgcacca cgtgcagcag tcgctgcgcg
      661 cccacaccct gttcacccgc gacgtcgact acatcgagcg ccagggcaag atcgtcatca
      721 tcgacgaatt caccggccgc atgatggaag gccgtcgcta ctcggaaggc ctgcaccagg
      781 ccctggaagc gaaggagcac gtcgaggtcc aggccgagaa ccagaccctc gcctcgatca
      841 cattccagaa ctacttccgc ctctacccaa agctcggcgg catgaccggc acggcgatga
      901 ccgaagcgga tgaactcgcc gagatctaca agctcgacgt ggtcgagatc cccaccaacg
      961 tcccctgcat ccgcaacgac gaagacgacg aggtctaccg gaccgccgcg gaaaaatatg
     1021 aagcggtcgc cgagctgatc gacgaggcgc gcaagaaagg ccagccggtc ctcgtcggca
     1081 ccacctcgat cgagaagagc gaggtcatcc atgaagtcct gcgcaagaag cgcatcccgc
     1141 acgctgtgct gaatgcccgc ttccacgaac aggaagccga gatcgtcgcg caagctggcg
     1201 cacccggtgc cgtgaccatc gccacgaaca tggccggccg cggcaccgac atcaaactcg
     1261 gcggcaacct ggagatgcgg ctcaagaagg agctcgacgg catcgacgat cccgcgatcc
     1321 acgccgcccg cgccgccgag atcaacgacg agatcgccgc cgcacaccag aaggtgaagg
     1381 aagccggtgg cctgttcgtc atcggcaccg aacgccacga aagtcgccgc atcgacaacc
     1441 agctgcgcgg ccgctccggc cgtcagggcg accccggccg ctcgcgcttc ttcctgtcgc
     1501 tcgaagatga cctgatgcgc atcttcggct ccgaccggat gggcggcatg ctgcagcgcc
     1561 tcggcctgaa ggaaggcgag gcgatcgtcc atccctggat caacaaggcg ctcgagaagg
     1621 cacagaagaa ggtcgaggca cgcaacttcg acacccgcaa gaacgtgctg aaatacgacg
     1681 acgtgatgaa ctcgcagcgc aaggaggtct acgcccagcg gaaggagttc atgaaggtct
     1741 cggacgtctc cgagacagtc gccgagatgc gcgccgaggt gatcgacaac ctcgtatcac
     1801 gccggatacc cgagaaaggc ttccacgaga attgggagct gaaagaactc acggaagaca
     1861 tccagcgcgt gctgaaccag acgctgccga tcgaggactg gggccgcgag gaaggcatcg
     1921 acgaaacgca tctgcgcgag cgcatcgagc aggcggccga tgccatgatg gcggccaagg
     1981 cggcaaatct cggccccgag ctgatgcgct tcatcgagaa gtcgctgctg atccaggtga
     2041 tggacgcagt ctggaaagag cacctctatg cgctggatca catgcgccag ggtatcggcc
     2101 tgcgcgccta tggccagcgc gatccgctga acgagtacaa atccgaggcc ttcgccctgt
     2161 tcaacgcgat gctggacgag ctgaaggaac gcgtgacgat gatgctggcg cgagtcgagc
     2221 tggcacccga tccggtccag cccccaccgc ccacgatcat gcaggaactg catccgcagc
     2281 cagctccatc ctttggtggc gaaccggatg cggcgatgga gggagccggc gcgatgacga
     2341 tcaccgcgcc gcagccgctg cgcagcgagg cggtcgatcc caatgacccc gccacctggc
     2401 gcaacgcctc ccgcaacgcc ccctgcccct gcggctccgg caagaaatac aaacactgcc
     2461 acggccgcct ggcataaggc cgccagcccc gaccgccccg acgcgggtca tcgcgaggag
     2521 cgtagcgcga agcaaacccg gccgggcttg cttcccactt gcacgaactc gtgaagttgc
     2581 gccatcgcga tgatgcttgc gatcgccgtg cccacccctg catacaatcg cccaggttcg
     2641 gggcactgca acgcagggaa cacgtcatgc ccgaaccgca atggcacacg ctgccgatca
     2701 acctcaccct cgccttcccc ctggtcccgc acatcgagcg ctaccagcgt atctacgatt
     2761 acctcaacac ccgcgacgag gtcatgctgg agctaagcgc gatcgacgcc gccacatcca
     2821 gcaagcaaac cttccgtgcc ttcctggaac gctgggaacg ccagctcggc ttctccgccg
     2881 acgttaaaac gatccacggc atcggcacca aagagaaaat cgccaagcac ttcaactctg
     2941 tgcctgacag gatcaattac gtcgacaacg agaacctgat cggcgaggcc gccttcatgc
     3001 agatcctctc ggcaaagcgc ccgatggtgg acgtcggcgc cggctctgaa cacggcctcc
     3061 tgacccaccg cgtccagtgg gtcatgatcg gcatgtggga ccgccgtacc cgaagcctgg
     3121 gcgtcggcga atacctagcc ccgatctatc agggcatggc caatccgaat gcccgcgcaa
     3181 aacaactggt ccgcgtagtc gatggcccga acgaaagacg caacctctgg gacatcgtcg
     3241 tcgacagctt caacaagaac gcgactcacc cggaatatct gcacgaccgc ttcgtgaaga
     3301 acgtgttccc cagcctctac gacaagtggg attgaccagg cacggcagcg cggctctaca
     3361 ccagccgcat gccgtctctc ctcgcggtct ccacgctgcc gccgtggccg gtgctgaacg
     3421 gctactcgct gcgcgtcgcg cacctgctgg aacaccttgc ggcgcaatgg tcgatcaccc
     3481 tgattgcgcc acctgttgaa gcgctgccac ccgccatcgc acggcacgtg ccggtcgcgc
     3541 tacctccaag cggcgtcacc tatccatggc gcttcgacca gaccagcctc cgcgccaccc
     3601 ttaaccacgt cgtcgccgaa acccacttcg accgcgcgct gatcttcccc ggcgccgaag
     3661 cgctgtggtt caaccgcatc gatctcccgc ccgccgtcat cgacatcatc gactgcaacc
     3721 cgctggagtt ctggcgcggc tttctctctt accgtggtct gcggcagcgc tatcgtgcct
     3781 tccgcgagat cgccatagcc acgcgatatg cacgccgcat ggtgcgtagc tacacagcaa
     3841 ccacctgcgt cggggaagcc gacacacgtt ggctccgccg cataggcggc cgtgacacgg
     3901 tgcacatgat cccgaacggt gtcgaagtgc cgatacatct caccgaagaa tccgccactc
     3961 ccacgctgat ctttaccggc gccctggatt accagcccaa tattgaagca gtgctgttcg
     4021 ctgctcagtc aatctggccg cgcatcgtcg ccgccaaacc cgacgctcgt ttcgtcatcg
     4081 caggccgcaa tccggtcccc gcgatacgtg cgctgtcgaa cgtggaaatc gctgccaatg
     4141 tgcccgacat gaccaaggtc ctcggcgaat catgggtcgc aatggcaccc atgcgctccg
     4201 gcgtcggcat caagaacaag gtgctggaag cctgggcctg cgcgcgcccc gtcgtgatga
     4261 gcacgctcgc caccaacggc ctgatcgtcc caccggacca tgcagcactc gtcggcacca
     4321 ccgccgatgc catggccggc gcagtactgc gcctgttcga tgatgcagac acgcgccacc
     4381 agctcggcaa gtcagcccga gagaacgtgc aacagcattt cacctgggcc cgtgcctcgg
     4441 cgcgcctcaa caaactgttg tgcacgtcct gcggagaaac ctgccccggg ctcgatcctg
     4501 gggtcaggtg aggtggggat ctccaatcct ccggcgaacc ctaacgcacc agcttttcga
     4561 tccccgcaaa gtcgattgcc ccaacgaagc tcttcagcgt cggatctggc gtatctttgt
     4621 tgccctcgac cttcacgatc accttatcgc cgatcagcgc catataactg ttgtcattct
     4681 cggaatacga catccgccgc ccgccaatca ccacagtctt catcccgcca gccgcagcga
     4741 atgggctgcc gagcagcgtg gccaaccctt gcagcatcgg tgaatcggcc atgatctgca
     4801 cctcgacctg ctgcgcttcc aaatgatagg tgcggctcac ggtggtgcct ccacccatca
     4861 tcgcaacgcc aacggtcgtc gtttccgcat catccgccgt ccatcccgcc ggtactggcg
     4921 gcaacaggtc cttcagcgta tcggcgcgtg cctgacgcag caggttctcc gccgcatcga
     4981 gcgccgcgag cgcggtttgc gtgtcatgct tctgatatgc cttcagcgcc tcattgatct
     5041 gatcggtcac atcatccgca tgcgccatcg gcgcagcagc gatcatcagc agcatcagtg
     5101 cgacgaggcg gctcatgcac tacaccgcaa gcagcgcctg cgcgcacgca tccagtatcg
     5161 cgtcggcgtc catgccatac tcccgataca gatccggaat atcgcccgtc tgcccgaagt
     5221 gatccggccc tagcggcacc acccgctgcc ctcgcaccga ccccagccac gcatgcgtcg
     5281 ccggatgccc atccaacacg gtcaccagcg ccgcacccgg cgccatgggc gccagcaacc
     5341 gttcgatatg cgacatcgct ccgcgatccc caccccgccg gccacgcatc gccgccagcc
     5401 accccgcatg cagccgatcg gcgctggtaa tcgcgaacaa cccagcgcca ggctcctccg
     5461 ccaccagttc agcgaatgcc gccgccgcct cgggcgccac cggcccctga tacccaatag
     5521 cgatgcgcgc gccatccgca ggaggcacca cccagtgcgc cccctcgatc accgccgccg
     5581 catccaacga gcgtgccggc tgctccagct gccgtgtaga caaccgtagc cacaccgcgg
     5641 acccgtccgg ccgctgcatg aactcgaacg catgacgcag caggatagcc agctcatcga
     5701 catgcgccgg ctcgaagctc gccagccgat ccgctgcgat cccgatcagc ggcgtgttga
     5761 tcgactgatg ctgtccgccc tccggcgcca gcgtcagccc ggacggcgtg cccaccagca
     5821 agaaccgcgc gtcctggtaa caggcataga tcaacgcatc cagcccgcgg ttgacgaacg
     5881 gatcatacac cgtccccacc ggcagcagcc gcgctccggt cattgccggg gtcaaccctg
     5941 ccgcacccag cagcaggaac aggttctgct ccgctatgcc aagctcgaca tgctgccctt
     6001 tcggcgacat tccccaccgc tgtgcactcg ccagcttcgc atcgcggaac acgtcgttgc
     6061 gtgtatgccg atcgaagatc ccacgccggt tcacccacgg cccaagattg gtggaaaccg
     6121 tcacatcggg cgacgtggtg acgatatgct cggccaacgc cttcagctcg ccctgcccgc
     6181 gcccgatctc cgcgagaatg tcgccaaacc ccgcctgcgt ggacatctta cgggccgcca
     6241 cctgcggcat cggcaaagcc gccggcaccg ccaccatcgg cgcggtcagc gttcgcccct
     6301 gcggcgtcag cggcgtggca aacgggcacg catcgatgaa cgcacgcagc tcttccgccg
     6361 gtacacccaa cccctcgaac atgtcccatt catgtcccgg ccggatgtcc atggaggcac
     6421 ggaacacctc catctgctcc ttcgtcatca gcccggcatg gttgtccttg tgccccgcga
     6481 acggcagccc catccccttg atcgtatatg caatgaagca cgtcggctga tcgccgtcgg
     6541 cctccgcgcg cagcgtatcg atcacggtct cgatgtcatg cccgccgaga ttggtcatca
     6601 acgcgccaag ctcatcatca gacatcgggt cgatgatcgc gcgcgccttc gaccgaccca
     6661 gatccgccag cagtgcctgc cgccacgaag ccccgccctg gaacgtcagc gccgagtaca
     6721 acgagttcgg gcagtcatcg atccaagccc gcagcgcctc gccgccatcg cgctcgaacg
     6781 ccgcctccag cttgcggcca tattttatgg tgacgacgtt ccaccccata tcgcggaaca
     6841 gcccttcgat gcggccgaac agccgatcgg gcaccacgct gtcgaggctc tgccgattgt
     6901 agtccacgat ccaccacaga ttgcggacgt cgtgcttcca cccctcgagc agcgcctcgt
     6961 agatgttgcc ctcatccagc tccgcatcgc cggcgatcgc gatatgccgt cccaccggca
     7021 cgtcggcgcc gccaagattg tgcaaccgca cataatccgc catcagcgcg gagaaagtcg
     7081 tcatcgccac gcccagcccg accgagccag tggaaaaatc cacctccggt ccgtccttca
     7141 cccgcgacgg gtaggactgc accccgccca actgccgcag cccctgcatc ttctccagcg
     7201 tctgccgccc gaacaggtag ttgatcgcat ggaataccgg cccggcatgc ggcttcaccg
     7261 caatccggtc ctgcggcctg gcaatctcga aatacagcgc cgtcatgatg gagatgaccg
     7321 aggcgcacga cgcctgatgt ccacccacct tcagcccatc gcgattgggc cggatgtggt
     7381 tggcattgtg gatcatccac gacgacagcc acagcagctt ccgctcgagc tgccgcagga
     7441 catgcagccg gtctgcgtcc cgggcagatg gagacagaac ggtctgtggc gtatccatgg
     7501 acgtcctcaa tagccggtga gcaagggcca gcttagcgcc ttgcaccggt cgcccgctac
     7561 ggcgcgctcc agacgaaact gccgagctgg aactggtcgt acacgtcaag atagcccttc
     7621 ttggtctcct cccatgcctt cacgttctgc ggcccgaatc ccaccttcgg caggcgcgcg
     7681 cagaaatcac gtggggaatg gcggaacgac agcacttcat tgcggtgacc cacattggga
     7741 taattcagga tccaggtgca ccacaggccc accgactccg ccgcatcatc agcagcgcag
     7801 aaatcaaccc agaccagatt gccgtcgccc tgaccgtcgg tctgcatctt gaccgtccgg
     7861 catttcatcc acgtgtacca gctcggcttc tgcagattga agtggcagcc ggtcttgccg
     7921 aaaatcttgc tgccgcccca gccactctcg gctgccgcgc aggccagcat cgccggcacc
     7981 ggcaggtcat tatcatacgc aactttcccg ccaccctcat tcaattgaaa caggaaacga
     8041 tactgcgcgt tgctaacctg cgagtccgca cccgcgtcgg ccatgaccag atcctcctga
     8101 tgtgccgttt gcacaccggg ccggtataac attaactttg cgtaattgag ctaattcccg
     8161 acggcgcact caggtttgtg atcgctcgcg ctcagctcca ccctcgcgca agccgccgca
     8221 ccgtcgtgga agcattgcgc ttcccttcgc ccgcgtaatc ctcgtgccag tcctcgatct
     8281 tcgccggatc gtagaggtaa ttcaccatca gcgtggcgct ctccttggca cctttctccg
     8341 acgtgtcgcc aagcatattg atccgctcca cccgcgcccc attcgacaga tggaaatgcg
     8401 ccaccggatc gcgcgcccgc ttctccccgt tcgtctccat catcagatac cgcgcgcaca
     8461 gccgtaccag caccggttcg gcggtctttc gcagcgccac ctcgcgccac catccccgct
     8521 tggcgacaat cgctgccagc gactctttgc ccgacggcgc cggcatagcc gaaccaagac
     8581 tcgccgcctc ctcgtccgac agcacgtccg tctcatccga agccagttgc gcatccagcc
     8641 acttccggaa cccagggata ggcgacagcg tcgcgaacgc cttcagattg cggaactccg
     8701 ccgacaactc ctccaccacg cgcttgatca gaaaattgcc gaagctgatc cccgctagcc
     8761 cgcgctggca gttgctgatc gaatagaaaa tcgccgtatc cgcctcgcgc gcatcctgca
     8821 ccggcgcctt ctcatccagc aggctctgca cgctgcccgc cagtcccttc accagcgcca
     8881 cctccacaaa gatcagcggc tcccccggca tgcgcggatg aaagaacgca tagcaccgcc
     8941 gatcggaatc cagccggttc ttgatgtcgc gccacgaccg gatcgcgtgc accgcctcgt
     9001 aatccaccag tttctcgagc aacgaggccg ggctgttcca gtcgatgcgc tgcaactcca
     9061 ggaacccgat atcgaaccac gccgccagca gcccgcgcag atccgcctcc agcgccgcaa
     9121 gcaaccgatc gtccttacga gaccgcaaca gaaactcgcg caaatcgacc aggaacttgc
     9181 ggccgtccgg aatcgtggtg aactgcgtca gcagcttgag ccgaggcggc tccagcgcgc
     9241 gccgcagatc ggccttcgcc accgccagct ccgccggatc agccgccgcc tgcacctcgg
     9301 cataggcccg ccccaccgcc tcggcatccg aatcgaacgc ggccagcgtg cgcaagaaat
     9361 ccttgcgccc agcatcatcc agttcttgat acgtttcagc caatttcgcc gcccggttgc
     9421 gcgcgctgac ctcgccgccg cgcccatcca ggcaggcccg catctgcgcc gcgatcggct
     9481 catccaccgg ccctgaagcc atatcgcgcc acaccgtcgt aatgcgccgg atagcacgat
     9541 ccagaagact cggggcagcg gcatcactca tacggtttgt cctcgctacg caatcgcatc
     9601 ataggcgctc acccgacata tgcggtgcac acgatgcgtc gaccagtgcc ggagcgatcg
     9661 cggacaaaag atcctcggca accagcccct gccccgctag agccccagca cgcccatgca
     9721 cccacgcacc cgccgcagcg gcttcccagg cgtccatccc ctgagccagc agcccgccaa
     9781 tcacccccga aagtacatcg cccgacccag ccgtcgccaa ccagggaggt gccgaagcat
     9841 tgatcgccac ccgaccatcg ggcgccgcca cgatcgtatc ggcccccttc agcagcacca
     9901 caccgcccgt ccgcgccgcc gccgcacgca ccgcatcaat ccgatccgcc cctgccgcac
     9961 caaacgcccg ggcaaactcg cccgcatgtg gcgtcatcac cgcggccccg cgcagcgcgt
    10021 ctggctctcc ggcaaat
//
LOCUS       DATGEM010000380         8917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_876927, whole genome shotgun sequence.
ACCESSION   DATGEM010000380 DATGEM010000000
VERSION     DATGEM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876927"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1466
                     /gene="murF"
                     /locus_tag="VKQ27_12085"
     CDS             84..1466
                     /gene="murF"
                     /locus_tag="VKQ27_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HLY89715.1"
                     /translation="MTALWTPHDLLEATGGVMDTPFAADGVSIDTRTLAGGDLFVALR
                     GERGDGHGFVAEAMAKGAAGAMVHRDVPDSSRLLLVDDTLAGLHRLGGYARVRFGGRL
                     IAITGSVGKTTVKEMLRTILGAFGPTHAAVASYNNHWGLPLTLARMPPGAAFCVTEIG
                     MNHPGEIAPLANLARPHVALITTVEKAHIGYLGSIEAIADEKAQLLRGLGTGGVAVLP
                     ADSPLLPRLRSAAGAARVVTFGTTAGADARLTAIASDADGSDVAAEVQGRAVRFRLNA
                     PGRHMAINALAAIAGAIELTMCPAEIISRITRALEEFSPLAGRGARRSIGVPGGTVLL
                     LDESYNGNPASVRAALAVLRLQPARRRIAVLGDMLELGEAGPAEHRALAADVAAAADL
                     LFACGPLMAELFAAAPPAARGAHTPDSASLAPVLARAVGPGDAILVKGSLGSRMKLVV
                     NALEQHAEAA"
     gene            1466..2554
                     /gene="mraY"
                     /locus_tag="VKQ27_12090"
     CDS             1466..2554
                     /gene="mraY"
                     /locus_tag="VKQ27_12090"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLY89716.1"
                     /translation="MLFDLARPLATQFILFNLFRYITFRSGAACLTALVVSFILGPPV
                     IRWLRSLQRNGQPIRTDGPERHLVDKKGTPTMGGVLILFALSISTLLWVDLRNLYVWA
                     VLFVTLGYGALGFADDYMKLSRRNTKGVSPKIKLIAQTLIGLIAAVWLMQLTRGALAT
                     SVAIPVFKEVLVQLGFLFPVFGMLVMLGASNAVNLTDGLDGLAIVPTIICAGVFALIA
                     YLVGNRIFADYLQLNPVPGVGELAVFCSALIGAGLGFLWFNAPPAAVFMGDTGSLSLG
                     GALGAIAVATKHEIVLLLVGGLFVVETVSVIIQVFWYKRTGRRVFLMAPLHHHFEKKG
                     WSEPTIVIRFWIISMILALVGLATLKIR"
     gene            2757..3884
                     /locus_tag="VKQ27_12095"
     CDS             2757..3884
                     /locus_tag="VKQ27_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HLY89717.1"
                     /translation="MPTLSRADNSVLGRWWWTVDRWTLGAIGVLIGFGYVMMLAASPA
                     VAERIGTPRDLFILKQVFFLAAAGAIVVSVSLLSPRSIRRVAIIGCIIALALTAATLV
                     TGVEIKGARRWIALPGMSVQPSEFLKPCFAIVAAWLISEGRRTPRFPGKFLAIGIYAV
                     IALLLKSQPDVGMLAVITAVFFVQLYVDGLNLILVGCAMVAFGFAGVGAYMFFSHVQR
                     RVQEFLHPGTGLGVDYQPHTALEAFGNGGLLGRGPGEGRVKDVLPDAHADFVFAVAGE
                     EFGMIVCLAIIAVFGFIVLRGLLRLLKEQDLFVVLSCTGLVTGFGLQAFVNMASSLQL
                     IPTKGMTLPFISYGGSSALAIALGMGMLLALTRRRHHGDVP"
     gene            3881..4984
                     /gene="murG"
                     /locus_tag="VKQ27_12100"
     CDS             3881..4984
                     /gene="murG"
                     /locus_tag="VKQ27_12100"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HLY89718.1"
                     /translation="MRGPAVFPIMIAAGGTGGHFFPAEALASELLARGRRVALMTDTR
                     SGGLQSPVFAGLEQYVISGAGIAGRGWRAGSAMLSLARGVAEARRIVVRSNAAVVVGF
                     GGYPCVAPVLATRLMRRRPPVVLHEQNAVLGRANRFLARHTDILALSFAATERVPETA
                     ATVVTGNPVRPTVRSVPYVPPSDVLRVLVLGGSLGARVFSDVVPPALVALPEGLRTRL
                     SVVQQCRAEDLAQVRAAYDAAEISAELSAFFPDVAERLAAAHLVIARAGASTVAELAV
                     AGRPAILVPLPGAIDDHQSANARALEQARGASVIAQRDFTPDALSGRLAALLASPDML
                     SHAALAAGSIARADATARLADVVEQLMRQEVSA"
     gene            4981..6414
                     /gene="murC"
                     /locus_tag="VKQ27_12105"
     CDS             4981..6414
                     /gene="murC"
                     /locus_tag="VKQ27_12105"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HLY89719.1"
                     /translation="MRALPLSIGTIHFVGIGGIGMSGIAEVLHNLGYAVQGSDIADST
                     NVRRLRDAGIPVAIGHDAANLGGAQVIVVSSAVKAENPEVATARARLIPVVRRAEMLA
                     ELMRLKWSVAIGGTHGKTTTTSMIAAVLEAARLDPTVINGGIINAYNTNTRLGAGDWM
                     VVEADESDGSFLRLPAVIAVVTNMDPEHLDHWGTPEAMVAGYDQFVANVPFYGFAVLC
                     IDHPAVQQMIPRLSDRRIITYGFSPQADVRADRVLPDKLGSTFEVTVTDRARNRTRKL
                     GPYRLPMLGAHNVQNALAAVAVGVEMEIDDDTLRSAFAGFGGVKRRFTKTGEAGGITI
                     IDDYGHHPVEIAATLKAARQAGARDVIAVVQPHRFTRLQTLFNEFCTCMNDAGTVIVA
                     DVYPAGEAPIPGVTRDALVEGLRARGHRSVVPLPSPARLAEMVFAIAKPGDFVMCLGA
                     GNITSWAAALPGELQELQQRRVSGSAA"
     gene            6414..7352
                     /gene="murB"
                     /locus_tag="VKQ27_12110"
     CDS             6414..7352
                     /gene="murB"
                     /locus_tag="VKQ27_12110"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HLY89720.1"
                     /translation="MMAAAAIPTFVDTLPPSRGRVQANAPLAPFTWFRVGGPAEALVR
                     PADAQDLAQFLRALPPEVPVHAIGACSNLIIRDGGLPGVVLRLARGFSTITAEADAVI
                     AGAAALDVTVAEHAAAAGLTGLEFLCGIPGSIGGAVVMNGGAYGGDVASVLDWAEVVT
                     RSGDQLRLSAANLAFAYRHSRLPHGAVVVRARLRARAGEPRAIAMRMADIRAAREASQ
                     PIRARTGGSTFRNPPDMKAWELIDAAGCRGLTRGGAQVSEKHCNFLINTGTATAADIE
                     GLGEEVRRRVHAATGVTLQWEIHRIGVPGSKPEAVA"
     gene            7349..8269
                     /locus_tag="VKQ27_12115"
     CDS             7349..8269
                     /locus_tag="VKQ27_12115"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HLY89721.1"
                     /translation="MTRVAVLYGGMSAEREVSLSTGIQVIPALREAGFDVTPIEVGED
                     LTAVIAALTPRPDAVFNALHGRFGEDGAIQGVLDWLAIPYTHSGVRASALAMDKVAAK
                     AVFAAAGLPVPRGRVVQQDELETTDPLPLPYVVKPISEGSSVGVEIIRNGDNRRAEVA
                     RSWRFGSAALVEEYIPGRELTVAVMGDRALAVTEIIADAGVFYDYESKYADGGSRHVI
                     PARIHADSYDRALSVALSAHRALGCRGATRCDFRYDDTAGEPGRLVLLELNTQPGLTP
                     TSLLPEQAAHHGMNFSQLCAWMVENAACRA"
     gene            8257..>8917
                     /locus_tag="VKQ27_12120"
     CDS             8257..>8917
                     /locus_tag="VKQ27_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLY89722.1"
                     /translation="MPRVARRNPRNSVNDRPARWKLWLRRQRKFLRPAGWGIFALVVV
                     LIGLATVRSAAPNGTLATLRERVGGVTALAGLRVTDVVIQGRANTPEPLLRAAIGVTK
                     GDPILGFSVELARERIESLSWVEQATVERQLPGTVVVYLKERRPFAIWQNQGKFVLID
                     RNGQVVANQNVSEFRQLPLVVGPGAPMDAALLIDALTDRPDLQKRVVAAVRVGERRWN
                     LQ"
BASE COUNT         1371 a         2624 c         3141 g         1781 t
ORIGIN      
        1 gggcacgagc aggggcagac tgtcgggggt gtggtcattc cgttcgatga tgtgagtgtg
       61 gtgcgtcggt tggcggggca agcatgactg ctctctggac cccgcatgac ctgctggagg
      121 cgactggcgg cgtgatggac acaccatttg ccgctgacgg cgtttccatc gatacgagaa
      181 cccttgccgg cggcgacctg ttcgtggcgc tacgtggcga gagaggcgat ggacacggct
      241 tcgtggccga ggccatggcg aagggtgcgg cgggcgcgat ggtgcatcgc gacgtgcctg
      301 actcgagccg cctgctgctg gtggacgata cgctggccgg actgcatcgg cttggcgggt
      361 atgcgcgggt gcggttcggc ggccgcctga tcgccatcac cggcagtgtc ggcaagacaa
      421 ccgtcaagga aatgctgcgg acaatcctgg gcgcattcgg gccaacgcat gccgcggtcg
      481 cctcctacaa caatcactgg gggctgccat tgacgctcgc acggatgccg cctggcgccg
      541 cgttctgcgt caccgagatc ggcatgaacc atcctggtga gatcgcgcct ctggccaatc
      601 ttgcccggcc gcatgtcgcc ctgatcacca ccgtggagaa ggcgcatatc ggctatctgg
      661 gttccattga agcgatcgcc gacgaaaaag cacagcttct gcgcgggctg ggaacgggcg
      721 gggttgccgt gctgccggca gactctccgc tgctgcctcg gttacggagc gcggcggggg
      781 cggcgcgtgt cgtcaccttc ggcacgacag ccggggctga tgcgaggctg actgcaatcg
      841 cgtcggatgc cgatggcagc gacgtcgcgg cagaggtgca gggccgcgcg gtaaggttcc
      901 ggctcaatgc acctggacgg catatggcga tcaacgcgct ggcggccatc gcaggcgcaa
      961 tcgaactgac aatgtgccca gcagagatca tcagccgtat tactcgtgcg ctggaagagt
     1021 tttctccgct cgcgggacgg ggcgcgcggc ggtcgatcgg ggtcccaggc gggacggtgc
     1081 tgctgttgga cgaaagctat aacgggaacc cggcctctgt ccgtgctgcg ctcgcggtgc
     1141 tgcgcctgca gcctgcgcgg cgccggatcg ctgtgctcgg cgatatgctg gaactgggag
     1201 aagcggggcc ggcggaacat cgtgcgctcg cggccgatgt tgctgcggca gcggacctgc
     1261 ttttcgcgtg cgggccgctg atggcggagc tgttcgcggc ggcgccaccg gctgctcgcg
     1321 gggcccatac gccggattct gcgtcgctgg caccagtgct ggcccgagcg gtcggaccgg
     1381 gtgatgcgat cctggtcaag ggaagcctgg gcagccgcat gaagctggtg gtcaatgcgc
     1441 tcgagcagca tgcggaggcc gcgtaatgct gttcgatctg gcgcgaccgc tcgccactca
     1501 gttcattctg ttcaatctgt tccgctacat cacgttccgc tcaggtgctg cgtgcctgac
     1561 ggctctggtc gtcagcttca tcctgggccc accggtcatc cggtggctcc gatcgctgca
     1621 gcggaacggt cagcccatca ggacggatgg tcccgaacgg catctggtcg acaagaaggg
     1681 cacgccgacg atgggtggcg tgctgatcct gttcgcgttg tcgatctcga cattgctgtg
     1741 ggtggatctg cgcaaccttt atgtgtgggc tgtgctgttc gtgacgctcg gctacggcgc
     1801 cctggggttt gccgacgatt acatgaagct gtcgcggcgg aacacgaaag gcgtgtcccc
     1861 gaagataaag ctcattgcgc agacgctgat cgggctgatc gccgcggtct ggctgatgca
     1921 actcacgcga ggcgcgttgg cgacgtctgt cgcgataccg gtgttcaagg aagttctggt
     1981 ccagcttggc tttctgttcc ctgtgttcgg catgctggtg atgcttggcg catcgaacgc
     2041 ggtgaacctg accgacgggt tggatggtct tgccatagtg cccaccatca tctgcgcagg
     2101 cgtctttgcg ctgatcgcgt atctggtggg aaaccgcatt ttcgcggact acctgcaact
     2161 gaatcccgtt cccggggtcg gcgagcttgc tgtgttctgt tcagcgttga ttggcgctgg
     2221 gcttgggttt ctctggttca atgcgccgcc ggcagcggta ttcatggggg ataccggatc
     2281 gctgtcattg ggcggcgcgc tcggtgcgat cgccgtggcg accaagcacg aaatcgtcct
     2341 gctgctggtt ggcggactct tcgtagtgga gacggtttcc gtcatcattc aggtgttctg
     2401 gtacaagcgg accgggcgac gagtgttcct gatggcgccg ctgcaccatc acttcgagaa
     2461 gaagggctgg tcggaaccca ccatagtgat ccgattctgg atcatctcga tgatactcgc
     2521 actggtcggt ctggcgacgc tgaagatccg atgatcacgc agcaccagtt acgggtccac
     2581 acagagatca ccgagatccg cggagcttca cggagacgtt ttagcggcgc tgcgtgcgac
     2641 ctgcgctgtg tttcctccgt gatgctctgt ggttccttgc gctctccgcg tggccccttc
     2701 cgccgaatcg caatgctggg cttccatgcg ggcgctttgc tggcagggct cgcctgatgc
     2761 cgactttatc tcgcgccgat aattccgtgc tgggccgctg gtggtggacg gtggaccgct
     2821 ggacgctcgg cgccatcggc gtgctgatcg gcttcggcta cgtcatgatg cttgcggcca
     2881 gtccggcggt tgccgagcgt atcggcacgc cgcgcgactt gttcatcctg aagcaggtct
     2941 ttttccttgc cgcggccggc gcgatcgtgg tgagcgtgtc actgctgtcg ccgcgcagca
     3001 tccgccgggt ggcgataatc ggctgtatca tcgccttggc acttaccgct gccacccttg
     3061 tgacgggtgt ggagatcaag ggcgcgcggc gctggatcgc gttgcccggg atgtcggtgc
     3121 agccgtcgga gttcctgaag ccgtgcttcg ccatcgtcgc ggcctggcta atcagcgagg
     3181 gtcggcgcac gccgcgcttc cccggcaaat tcttggcaat cggcatctac gccgtgatcg
     3241 cgctgctcct gaagtcgcag ccggatgtgg gcatgctcgc ggtgatcacc gcggtgttct
     3301 ttgtccagct ttatgttgat ggtctgaacc tgatcctggt gggctgcgcg atggtggcct
     3361 tcggttttgc cggcgtgggc gcctacatgt tcttctcgca cgtgcagagg cgcgtgcagg
     3421 agttcctgca cccgggcacc ggactcggcg ttgactatca gccgcacaca gcgctggaag
     3481 cattcggcaa cggcgggctg ctggggcgag gtcctggcga gggcagagtg aaggacgtat
     3541 tgcccgacgc acatgcggac ttcgtgttcg cggtggcagg cgaggaattc ggcatgatcg
     3601 tctgcctcgc catcatcgct gtattcgggt ttatcgtcct gcgcggactg ctgcggctgc
     3661 tgaaggaaca ggacctgttt gtcgtcctgt cctgcaccgg actggtcacc ggcttcgggc
     3721 tgcaggcgtt tgtgaacatg gcgtcgtcgc tgcagcttat cccaaccaag ggaatgacgt
     3781 tgccgttcat ctcctacggc ggttcgtcag ctctggcgat tgcgctgggc atgggcatgc
     3841 tgctggcact gacgcgccgc cgacatcatg gcgacgtgcc atgaggggac cggcggtgtt
     3901 tcccatcatg atcgcggctg gcggcactgg aggacacttc ttcccagccg aggcgctggc
     3961 gagcgaactg ctggcgcgcg gacgtcgtgt ggcgctgatg accgatacgc gctcgggagg
     4021 gctgcaaagc cccgtgttcg cggggctgga gcaatacgta atcagcggtg ccggcatcgc
     4081 gggacgcgga tggagggcgg gtagcgcaat gctgtcgctc gcacgtgggg tagcggaggc
     4141 gcggcgcatc gtggtgcgca gcaacgcggc ggtggtggtg gggtttggcg gttatccgtg
     4201 cgtggcgccc gtgctggcga cgcgactgat gcggcggcgg ccacctgtgg ttctgcacga
     4261 gcagaatgca gtgctgggac gagccaaccg attcctggca cggcacaccg atattctggc
     4321 gctgagtttc gcggcgaccg agcgcgtgcc ggagacggcg gccaccgttg tcacggggaa
     4381 cccggtgcgg ccgacagtcc gctctgtgcc gtatgtgccg cccagcgatg tgctgcgcgt
     4441 gctggttctg ggtggatcgc tcggcgcgcg tgtgttcagc gatgtggtgc cgccggcatt
     4501 ggtggcattg ccagaagggc tgcggactcg gctttccgtt gtgcagcagt gccgtgcgga
     4561 ggatctggct caggtgcgtg ctgcgtatga tgcggcggag atttccgcgg agctgtcggc
     4621 attctttccg gatgtggcgg aacggctggc cgcggcgcat ctggtaatcg cgcgggccgg
     4681 ggcatcgacg gttgccgaat tggccgtcgc ggggcggccg gcgatcctgg tgccgttgcc
     4741 gggagcaatc gacgatcatc aatcggcgaa tgcgcgggca ctggaacagg cgcgaggcgc
     4801 gtcggtgatc gcgcagcgtg acttcacgcc tgatgcattg tccggtcggt tggccgcgct
     4861 gctggcgtcg cctgacatgc tgagccatgc ggcgctggcg gccggatcga tcgcacgggc
     4921 ggacgcaacg gcacgactgg ctgacgttgt cgaacagctc atgcgtcagg aggtgtccgc
     4981 gtgagagctc ttcctctttc catcggcacc atccatttcg taggcattgg cggcatcggc
     5041 atgtcgggca tcgccgaggt actgcacaat ctcggctacg cagtgcaagg cagcgacatc
     5101 gcggattcca cgaatgtccg gcgattgcgc gacgccggta tcccggtggc cattgggcat
     5161 gatgctgcca acctgggcgg tgcgcaggtc attgtggttt cgtcggcagt gaaggccgag
     5221 aatccggagg tcgccacggc acgcgcgcgg ctgatccctg tggtgcgacg agccgagatg
     5281 ctcgccgaac tgatgcgttt gaaatggtcg gtggccattg gcggtaccca tgggaagacc
     5341 acgaccacaa gcatgattgc ggcggtactc gaagccgcgc gcctggatcc cacagtgatc
     5401 aacggcggca tcatcaacgc ctacaacacc aatacgcggc ttggcgccgg cgactggatg
     5461 gtggtcgagg cggatgaaag cgatggcagc ttcctgcggc tgccggcggt gatcgccgtg
     5521 gtcacgaata tggaccccga acatctcgat cattggggga cgccggaggc gatggtggcc
     5581 ggctacgatc agttcgtcgc caacgtgccg ttctatggct tcgccgtgtt gtgcatcgat
     5641 catcccgcgg tgcagcagat gattccgcgc ctctctgatc ggcgtatcat cacttacggg
     5701 ttctcaccgc aggccgatgt gcgcgcggac cgcgtgttgc cggacaagct tgggtcgacg
     5761 ttcgaagtca cggtgacgga tcgtgcgcgc aacaggacgc ggaagctggg gccgtatcga
     5821 ttgccgatgc tcggcgcaca caacgtgcag aatgcattgg cggccgtggc tgtcggcgtc
     5881 gaaatggaaa tcgacgacga cacgctgcgc agtgccttcg ctggttttgg tggggtgaag
     5941 cggcggttca ccaagaccgg cgaggcaggc ggcatcacga tcatcgatga ctacgggcat
     6001 catccggtgg aaatcgccgc gacgttgaag gcagcgcggc aggcaggggc gcgcgatgtg
     6061 atcgcggtcg tgcagccgca tcgctttact cgcttgcaaa cgttgttcaa cgagttctgc
     6121 acctgcatga atgatgcggg tactgtgatc gtcgccgacg tctatcctgc tggcgaggcg
     6181 ccgataccgg gtgtgacacg tgacgcgctg gtggagggat tgcgggcacg cggtcatcgc
     6241 agcgtggtgc cgctgccgtc gccggcacgt ctggccgaga tggtattcgc gatcgccaag
     6301 cccggcgatt tcgtcatgtg cctgggtgcc ggcaacatca ccagctgggc ggcggcgctg
     6361 cctggcgaac tgcaggaact tcagcagcgc cgtgtttccg ggagtgcggc gtgatgatgg
     6421 ccgccgccgc tatcccgacg tttgtcgata cgctgccgcc gtcgcgcggg cgtgtccagg
     6481 ccaacgcgcc cctggcgccg ttcacctggt tccgtgtcgg cggcccggcc gaggcattgg
     6541 tgcgtccggc cgacgcgcag gatctggcgc agttcctgcg ggcgttgccg cctgaggttc
     6601 cggtccacgc gattggtgcc tgttccaacc tgattatccg cgatggcgga ctgcctggcg
     6661 tggtgctgcg gcttgcccgc gggttcagca cgatcactgc ggaagccgat gcggtgatag
     6721 caggggcagc tgcattggac gtgaccgtgg ctgaacatgc ggcagccgcg ggattaaccg
     6781 ggctggagtt tctctgtggc attccgggca gcatcggcgg cgccgtggtg atgaacggcg
     6841 gcgcctacgg cggagacgtt gcttccgttc tggattgggc ggaagtggtt acgcgaagcg
     6901 gcgatcaact tcgcctttct gcggcgaatc tggcgttcgc ttaccggcat tcccgattgc
     6961 cgcatggagc ggttgtggtg cgagcccggt tgcgcgcgcg ggcaggcgag cctcgggcga
     7021 tcgccatgcg catggcggat atccgtgcgg cccgcgaagc ttcgcagccg atccgggcgc
     7081 gaactggtgg gtccacgttc cgcaatccac cggacatgaa ggcttgggag ttgattgatg
     7141 ccgccggctg ccgcggcctg acgcgcggtg gtgcacaggt atcggagaag cactgcaatt
     7201 tcctgatcaa taccgggact gcgacggctg cggatatcga aggccttggc gaggaagtgc
     7261 ggcgacgtgt gcatgccgcc accggtgtga ccttgcaatg ggagatccat cgcatcggcg
     7321 tgccgggcag caaaccggag gcggtggcat gacccgcgtc gctgtcctct acggcggcat
     7381 gtctgctgaa cgcgaagtca gcttgtccac cggaatacag gtcatcccgg cgctgcgcga
     7441 agccggcttc gatgtgacac cgatcgaagt cggcgaggac ctgaccgccg tcatcgctgc
     7501 gctgacgccg cgtcccgatg ccgtgttcaa cgcgttgcat gggcgctttg gtgaagatgg
     7561 agcgatccag ggcgtgctcg actggctcgc aatcccttat acccattctg gtgtccgcgc
     7621 gtctgcgctt gcgatggaca aggttgccgc gaaggcggtg ttcgccgccg cgggtctgcc
     7681 tgtgccgcgt ggccgggtgg tacagcaaga tgaattggaa accaccgatc ctctgccgtt
     7741 gccttacgtg gtgaagccga tcagcgaagg ttcatcggtt ggcgtcgaga tcatccgcaa
     7801 tggcgacaac cgacgagcag aagttgcgcg ctcgtggcgg ttcggcagcg cggcgctggt
     7861 cgaagagtac atcccgggcc gcgaactcac cgtggcggtc atgggggatc gcgcgctcgc
     7921 cgtgacggag atcatcgcgg acgccggcgt cttctacgac tacgaatcaa aatatgccga
     7981 cggcggatcg cgccacgtga ttccggcacg tattcacgcc gatagctacg accgcgccct
     8041 cagcgtggcg ctttcggcgc atcgtgcgct cggctgccgt ggcgccacac gctgcgactt
     8101 ccgttatgat gatacggcag gcgagcccgg gcgcctggtc ttgctggagc ttaacacgca
     8161 gccaggcctg acgccgactt ctctgctgcc ggaacaggcc gcgcatcacg gcatgaattt
     8221 ctcacagctt tgcgcctgga tggtggagaa cgcggcatgc cgcgcgtagc tcgccgcaat
     8281 ccgcgcaact cggtcaatga ccggccggca cggtggaagc tatggctgcg cagacaacga
     8341 aagttcctgc gtccggcggg ctggggcatt ttcgcattgg tcgtcgtact gatcgggctg
     8401 gcgacggtgc ggtcggcggc gcctaatggt acgcttgcga cgttgcgcga acgcgtaggc
     8461 ggagtgaccg cccttgccgg gttgcgtgtt accgatgtgg tgattcaggg gcgtgccaat
     8521 acgccggagc cactgctgcg tgccgcgatc ggcgtgacca agggcgaccc gatccttggc
     8581 ttttcggttg aattggcgcg cgagcggatc gaatcgcttt cctgggtgga acaggcgacg
     8641 gtcgagcgtc agctccctgg aactgtggtt gtctatctga aagagcggcg accgttcgcg
     8701 atctggcaga accagggcaa gttcgtgttg atcgaccgca atggccaggt ggttgcgaac
     8761 cagaacgtct ccgaattccg gcagcttccg cttgttgtgg ggccgggcgc gccgatggac
     8821 gccgccctgc tgatcgatgc cctcaccgat cggcccgatc tgcagaagcg cgttgtcgcc
     8881 gcggtacggg tgggtgagcg gcgttggaac ctgcaac
//
LOCUS       DATGEM010000381         6773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_857030, whole genome shotgun sequence.
ACCESSION   DATGEM010000381 DATGEM010000000
VERSION     DATGEM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857030"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VKQ27_12125"
     CDS             complement(<1..553)
                     /locus_tag="VKQ27_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89723.1"
                     /translation="MTRSAVRWSWQLPFYYGWVIIGIAFITMAIAVTARTAFSLLLPP
                     LIGEFGWDRGLTAGAFSFGFLISALLSPVAGRLMDRNGPRVVIGTGVCLMSLGLFTAP
                     LIRTPWQLYATLGFLVAVGAGFMTYTAHSLFLPHWFVRRRALAISIAFAGAGIGGIVL
                     LPWLQTIILREGWRASCSAMGYLV"
     gene            complement(550..1521)
                     /locus_tag="VKQ27_12130"
     CDS             complement(550..1521)
                     /locus_tag="VKQ27_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLY89724.1"
                     /translation="MKIAFAGAFAARLAEPVRAKLTMPCDITVDDEVGTIQHLNDADV
                     LVAMGFSARMAEAAQRLRLVQVPGAGLDRIDRAALRPGTYLANAYGHEAGIAEYVIGA
                     MIALTRTFARLDARLRRGEWESQWAIGHPAPPLWPELGGKTLGILGFGHIGRALARRA
                     LAFDMRICAMRSRAMPDAPAGMAFVGGPERLDEVLKEADFLAATLPLSPATHHLLDER
                     RLRLLKPTAILINVGRAEICEEVALYQTLVSGRIAGAALDVWYRYPGTGEPTLPATQP
                     FHTLGNVMMTPHVSGWTEGMLDARARLIAENIARIARGEPPLNAIDA"
     gene            1629..2435
                     /locus_tag="VKQ27_12135"
     CDS             1629..2435
                     /locus_tag="VKQ27_12135"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY89725.1"
                     /translation="MKTPQAFASRLVWWRKRRGLSQLQLALAAGCSQRHVSFLELGRT
                     NPSREMVLRLSATLEMPLRQSNELLLAAGYAPVWTEADLGALALAPVRDALGYMLRQQ
                     EPYPAVVVDRRWNLLQANQGAVALVEFLVGPVAPGASINLADALVAPDVLRPCLTNWE
                     DVVRFFLRSVQADAAADATTETAALLERLLGYPDVRPLIIAGVLPVVDGPVLPMHFAK
                     GGIMLRLFTTIATLGTPQDITVQELRIESFFPMDEATRDVFRGWAGLRED"
     gene            complement(2421..2882)
                     /locus_tag="VKQ27_12140"
     CDS             complement(2421..2882)
                     /locus_tag="VKQ27_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89726.1"
                     /translation="MDLMDNALRCPQSHNLIICLGEGFALLKDVEDRAERQRRNWIEA
                     YRGLGDAGAVCRRFGVSPIDRVCQLADKTPLWAAVGDAYDASKERIKIRHHAVVVALQ
                     ALKSSLGTAQVIVRPDPGGGSCKTVANIEGLGTLDIQALPGVNNPVASIFS"
     gene            complement(3546..4487)
                     /locus_tag="VKQ27_12145"
     CDS             complement(3546..4487)
                     /locus_tag="VKQ27_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89727.1"
                     /translation="MTANTLRVLAAYGAAVLLFVIGNWVHPGFASFDSVTSILLIASF
                     VGLVAAGQCFVILIGGIDLSVPWVLNAAAILLVTESLGEDSRALYAVAVTLGMGVLAG
                     LVNGIGVVWFGVPAVVMTLAMNGIIEGLALGLSGGMTCTACASYAPPVIAAVVHDVWL
                     GIPAALWLWLAVIAVVTFVLSLTSFGRTTYAIGNNARAAYLAGINVNLTTAALYALSG
                     LFSAMAGILLVGFGGQASLGMGAPYLFQSIAAVVIGGVSILGGSGSYLGAAAGAASLT
                     ALVSVLLALNMPDYGRSIIYGVVILALLLLYGREQRE"
     gene            complement(4484..5488)
                     /locus_tag="VKQ27_12150"
     CDS             complement(4484..5488)
                     /locus_tag="VKQ27_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89728.1"
                     /translation="MPSDATIPPRVVQRRPRAAHMLGQNAGLILAAFILVLCIAGFAA
                     MFQIQQQRLPGNFEVTSTVNNTMPLALAAIGQSIVVLTRGIDLSVGGVVDLSNALAAI
                     WLTGGVLHMIGISFLIVLIGAGAGLINGLLVGLGRLQPIVVTLATLSIFQGLAIRVLP
                     QPGGEVPPGYTIFLANPNQPTALVFLAVAIVAWMALRRSRLGVTIFAIGNDEASAVTL
                     GVNAIRTKVFAYMIGGALYAVAGLFLAATATAGDATTGDGYTLTSIVAVVLGGVNLFG
                     GRGSAVGAICGAFITTMIVNILFFAHINPLYQSFYEGLFLVVAVVFGALIGRLMRGIR
                     "
     gene            complement(5478..>6773)
                     /locus_tag="VKQ27_12155"
     CDS             complement(5478..>6773)
                     /locus_tag="VKQ27_12155"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLY89729.1"
                     /translation="RLAGPADAVAHGIGTVFQELTLLPWMTVAENLLIGREPRAFGFI
                     RRAELGPAAERTLAELGIYHIDPRALAADISLAERQIIEIVRVIVRRPRVLLLDEPTS
                     SLVEREVEWLFARIRELSAAGTAVVFTSHRWKEITSIADRITIFRNGTEVGTFTGIDE
                     SEAITWMTGQQVEALYPACPPIPANARPALELAEATVNGSARLGFSLAAGEILGVGGL
                     AGQGHREMFLGLFGAPPLRHGTIRIDGKPTRIASPADAIRANLGIALVPEDRKSEGLL
                     LPLSVRDNLTLPILPRLSRLGVIRSAAEKRISTEMIQQLGIRTPGMAQPVGALSGGNQ
                     QKVLVGRWLLADSRILLLYDVTRGVDIATKHEIYELMMRLAGEGRAILFYSSDTEELA
                     HLCHRVLVLRYGRIAAELAPPALTAEAIVSAAIHDAHAE"
BASE COUNT         1356 a         2162 c         2122 g         1133 t
ORIGIN      
        1 gcaccaggta gcccatggcc gagcatgatg cgcgccagcc ctcgcgcagg atgatcgtct
       61 gaagccatgg cagcaaaacg atcccgccaa tccccgcgcc ggcgaaggcg atgctgatcg
      121 ccagtgcgcg ccggcgcacg aaccagtggg gcaggaacag cgaatgcgcc gtgtaggtca
      181 tgaagcctgc gcccacggcc accaggaagc cgagcgtcgc atacaattgc catggcgttc
      241 gtatcaacgg cgcggtgaac aggcccaggc tcatcaggca cacgcctgtg ccgatgacaa
      301 cgcgtgggcc gttgcggtcc atcaaccgtc cggctaccgg actcaacagt gcggaaatca
      361 agaagccgaa tgagaatgcg ccggccgtca ggccacgatc ccaaccgaac tcgccgatca
      421 gcggcggcag caacagtgag aacgccgtgc gagccgtgac cgcgattgcc atcgtgatga
      481 aggctatgcc gatgatcacc cagccgtagt agaacggcag ctgccaggac cagcgcaccg
      541 cggacctcgt cacgcgtcga tcgcattgag cggcggctcg cctcgcgcaa tcctggcgat
      601 gttctcggcg atcagccggg cacgtgcatc gagcatgcct tcggtccagc cggacacgtg
      661 cggcgtcatc ataacgttgc cgagcgtatg gaacggttgc gtcgcgggga gcgtcggttc
      721 gcccgtgcct ggataacgat accacacatc cagggccgcg cctgcaatgc gccctgacac
      781 aagcgtctgg tagagcgcta cctcttcgca gatctcggca cggccaacat tgatcaggat
      841 cgccgttggc ttcagcaatc gcaggcgccg ctcgtccagc agatggtgcg tcgccggcga
      901 cagaggcagt gtggcggcga gaaagtctgc ctctttcagc acttcatcca gccgctcggg
      961 accaccaaca aatgccatgc cagcgggtgc atctggcatc gcccggctcc gcatcgcgca
     1021 gatacgcatg tcgaacgcca atgcccggcg tgcgagcgcc cgtccgatat ggccgaagcc
     1081 taggataccc aatgtcttgc cgcccagttc cggccagagc ggcggtgcgg gatgaccaat
     1141 ggcccattgg ctctcccact caccccgccg cagccgggca tcgagacgcg cgaaggtccg
     1201 agtgagcgcg atcatcgcgc cgatgacgta ttcagcgatc ccggcttcgt gcccgtatgc
     1261 attcgccagg tacgtgccgg gccgcagcgc ggcacgatcg atccgatcga gacccgcgcc
     1321 aggcacctgc accaatcgca gccgttgagc tgcctcagcc atgcgggccg agaaccccat
     1381 tgcgaccagc acgtcggcat cgttcagatg ctggatggtg cccacctcat catcgacggt
     1441 aatatcgcac ggcatcgtca gcttcgcgcg gactggttcg gcaagccgcg ccgcgaacgc
     1501 gcccgcaaag gcaatcttca ttccgtggtc ctcgctgtgg cgcgcggcgc gtgttgcgtg
     1561 ttgcggcggc gtccgactca tgcactatcc cgcgcggttg ccgatgttca actacatgcc
     1621 aggtagtcat gaaaacgccg caagcctttg catcgcgtct ggtgtggtgg cggaagcgac
     1681 gcgggctgtc gcagcttcaa ctcgcgctgg ccgcgggctg ttcgcagcgc cacgtcagtt
     1741 tcctcgaact tggccgcacc aatccaagcc gcgagatggt gctgcggctg agcgcaacgc
     1801 tggagatgcc gttgcgacag tcgaacgagc tgctgctcgc cgcaggatat gcgccggtgt
     1861 ggaccgaagc agatctgggg gcgctggcgc tggcgccggt acgcgacgcg ctcggctata
     1921 tgttgcggca gcaggagcct tatccggctg tcgtggtgga tcgacgatgg aacctgttgc
     1981 aggccaatca gggcgcggtc gcgctggtgg aattcctggt gggaccggtg gcgccgggtg
     2041 cctcgatcaa tctcgccgat gcgcttgtcg cgcccgatgt gctgcggccc tgtctgacca
     2101 actgggaaga cgtcgtgcgc ttcttcctgc gcagcgtgca ggctgatgcg gctgcggatg
     2161 ccaccacgga aacggcggcg cttctggaac ggctgctcgg ctatccggat gtgcgtccac
     2221 ttataatagc cggcgtgctt cctgtggtgg atggcccagt gctgccgatg cacttcgcga
     2281 agggggggat catgctgcgg ttgttcacga cgattgcgac acttggcacg ccgcaggaca
     2341 tcacagtaca ggaactgcgg atcgagagct tcttcccgat ggacgaggca acgcgtgacg
     2401 tgttccgagg ctgggcaggg ctacgagaag attgaggcaa ccgggttgtt cacgccgggc
     2461 agcgcctgga tatccagcgt gcccaggcct tcaatgttcg caactgtctt gcagctcccc
     2521 ccgccgggat cgggacgcac gatcacttgt gccgttccta gagatgattt caacgcttgt
     2581 aacgctacaa caacggcatg gtgccggatt ttgatccgct ctttcgaggc atcgtaagca
     2641 tcaccgacag ctgcccatag cggggtcttg tcggccagct gacacactcg gtcgattgga
     2701 gagacaccga agcggcgaca cactgcaccc gcgtcgccaa gaccgcgata cgcctcgatc
     2761 cagttgcgtc gctggcgctc cgcgcgatcc tcaacgtctt tcaacagagc aaatccttca
     2821 ccgaggcaga tgatgaggtt gtgggattgt gggcaacgca aagcgttgtc catcaagtcc
     2881 ataccctgcc agatccggtg aaacgatgtc tatgaaccgg acacgtgaag gtatcgagct
     2941 tgtgcaccag tcgctccgta tgcacccaac acaacgcatg gcggccaacc agaaactgac
     3001 cggcaacgtc gctgacgatg accgtgttga gcaggagtcc gtgcgcatgg atgctggccc
     3061 agatggctcc gcatcgcggc gatagcggat cacctgggca cgcagcacca gatcccgcac
     3121 caggtagccg gcgtagccct tgaagcgtga ccctgccgga acgcgccggc aactttgatc
     3181 acacgctcct cgaccctcac acacgcggcg tcaatttgcc gcgccgacgc cgcttcgccc
     3241 gcttgcctcc gctcgctcca actcggcaac tacgccaaat agggcgacca tcagagcctt
     3301 catctcggca atagaaagcc catcaatgtc cggcgctggt gccatctcca gaatgaatca
     3361 taaccgctcg cgtggggggt aacgaaaact tccttcccgc caccggtttt gccccggtta
     3421 ccagcagcac gacaccgttc atgctcaccc actgtgatac aggatccaga acgcggttcc
     3481 ggtggcggcc aattcatgca gttccttagc gaagttagct ttttccccgc tcgcccatgc
     3541 cgtcactact ctcgttgttc tcgcccatac agcagcaaca gcgccagaat gaccacgccg
     3601 tagatgatgc tgcggccata gtccggcata ttcagcgcga gcagtacact taccagcgcg
     3661 gtcaggctgg cggcgccggc ggcagcgccg agatagctgc cgctgcctcc caggatgctg
     3721 accccaccga tgaccacggc ggcaatcgat tggaacaggt acggtgcccc catacctaat
     3781 gaggcctgac cgccgaagcc caccagcaga atgccggcca tggcggagaa caatccgctt
     3841 agcgcataga gcgccgcggt ggtcaggttg acattgatgc cggccagata agctgcacgg
     3901 gcattgttgc caattgcgta ggtggttcgg ccgaagctcg tcaggctcag aacgaacgtc
     3961 acgactgcga tcactgccag ccacaaccac aatgcggcgg ggatgcccag ccacacatca
     4021 tgcaccacgg cggcgatcac cggtggtgcg taggaagcgc aggccgtgca cgtcatgccg
     4081 ccgctgaggc ccagtgcgag gccctcaatg atgccgttca ttgccagcgt catcaccacg
     4141 gccgggacac caaaccagac gacaccgatc ccgttcacca ggccggccaa cactcccatg
     4201 ccgagagtca ccgcaacggc atagagagcc cggctatcct cacccaggga ctctgtcacc
     4261 agtaggatcg ctgccgcgtt caacacccat ggcaccgaaa ggtcgattcc accgatgagg
     4321 atgacgaagc attggccggc ggccaccagc ccgacgaatg acgcgatcag caggatcgag
     4381 gtcacgctgt cgaagctggc gaacccggga tgcacccaat tgccaatgac gaacagcaac
     4441 accgcggcgc cataagcggc tagcacacgt agcgtattcg cggtcatcgt atgcctcgca
     4501 tcagccgccc aatcagggca ccgaacacca cggcgacgac caggaaaagg ccttcgtaga
     4561 aagactgata gagcggattg atgtgggcga agaacaggat gttgacgatc atggtggtga
     4621 tgaatgcacc gcagatcgcg cctaccgcgc tgccacgtcc gccgaacaaa ttcacgccgc
     4681 ccagcacgac cgcgacgatt gaggtcagcg tgtagccatc gccggtcgtg gcgtcgcccg
     4741 cggtcgcggt cgcggccagg aacaatccag ccaccgcgta cagggcaccg ccgatcatgt
     4801 aggcgaagac cttcgtgcgg atcgcgttca cgcccagcgt caccgccgag gcctcgtcat
     4861 tgccgatagc gaagatggtg acgccgaggc gtgaacgtcg cagcgccatc cacgcaacga
     4921 ttgcaacggc gagaaagacc aacgcggtag gttgattagg attagccaag aaaatggtgt
     4981 agccgggcgg cacctcgccg ccgggctgcg gcagtacgcg gatcgcgagt ccctggaaga
     5041 tcgacagtgt cgccagcgtc actacaatcg gctgcaggcg ccccaggccg acgagcaacc
     5101 cgttgatcag tcccgccccc gcgccgatca acacgattaa gaatgagatg ccgatcatgt
     5161 gcagcacgcc gccagtcagc cagatcgcgg ccagcgcatt gctcaggtcg accacaccgc
     5221 caacagacaa atcgatcccg cgcgtcagca ccacaatgga ctggccgatt gctgccagtg
     5281 ccagcggcat ggtgttattg accgtggagg tgacttcgaa attgcctggc agccgctgct
     5341 gctggatctg aaacatcgcc gcgaatccgg cgatgcagag caccaggatg aaggcggcca
     5401 ggattaggcc ggcgttctgg ccgagcatgt gtgcggcacg cgggcgccgt tgcacgaccc
     5461 gcggcgggat ggtggcgtca ctcggcatgg gcgtcgtgga tcgcggcgga gacgatcgcc
     5521 tccgcggtca gtgcgggagg cgccagttcg gcggcgatgc ggccatagcg cagcaccagc
     5581 acgcgatggc agagatgggc gagttcctcg gtgtcggagg aatagaacag gatcgccctg
     5641 ccctcaccag cgaggcgcat catcagttca tagatttcat gttttgtggc gatgtcgacg
     5701 ccgcgggtca catcatagag caacaggatg cgcgagtcgg ccagcagcca gcggccaacc
     5761 agcaccttct gctgattgcc accggaaagc gcaccgactg gctgggccat gcccggcgtc
     5821 cgaatcccca gctgttgaat catctccgtg cttatccgtt tttccgcggc gctccggatc
     5881 acgccgagcc gggacaggcg aggcaggatc ggcaaggtga ggttgtcgcg tactgacagg
     5941 ggtagcagca ggccctcgga cttacggtcc tccgggacca gggcaatgcc gaggttggcg
     6001 cggatggcgt cagcgggact ggcgatgcgc gttggcttgc cgtcgatacg gatggtgccg
     6061 tgacgcagcg gtggcgcgcc gaacaagccc aggaacattt cgcgatgacc ttggccggcg
     6121 aggccgccga caccgaggat ttccccggcg gccaggctaa aaccaaggcg cgcactgccg
     6181 ttcaccgtag cctccgccag ttccagcgcc gggcgagcgt tggcaggaat cggagggcag
     6241 gcaggatata gcgcctcgac ttgctggccg gtcatccagg tgatggcttc ggactcgtcg
     6301 atcccagtga aggtgcccac ctcagtgccg ttgcggaaga tggtgatgcg atcggcgatc
     6361 gacgtgatct ccttccaacg gtgggaggtg aacaccaccg cggtgccggc tgcgctgagt
     6421 tcgcggatgc gcgcgaacaa ccattccacc tcacgctcga ccaatgatga tgtgggttcg
     6481 tccaatagca ggacgcgcgg gcggcggacg atgacgcgca caatctcgat aatctggcgt
     6541 tcggcgagag agatgtccgc ggcaagtgcg cgcggatcaa tatgatagat gccgagttcc
     6601 gcaagcgtac gctctgctgc agggccgagc tcggcgcggc ggataaagcc gaaagcccgc
     6661 ggttcacggc cgatcaggag gttctcagcg acggtcatcc agggcagcag agttagctcc
     6721 tggaacacgg tgccaatgcc gtgcgcaacg gcgtcggcgg ggccggcgag acg
//
LOCUS       DATGEM010000382         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_5023, whole genome shotgun sequence.
ACCESSION   DATGEM010000382 DATGEM010000000
VERSION     DATGEM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VKQ27_12160"
     CDS             complement(<1..1181)
                     /locus_tag="VKQ27_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLY89730.1"
                     /translation="MNVAPPPGSLRPEPVDLPPDVEVSRTRALVGNIANILLGKVVTL
                     VLATLAFGVALATFIILARGAPLSPKPGITVGLVLANLIAILLLVAVLAGRLTRVWVE
                     RRRGSAGSRLHVRLVLLFGGVAVAPAIVVACFAVAFFHFGIQSWFNDPVRQAINESLQ
                     VSRGYLEEHRDNIRSVALEMANDLTHAGQFLSADPSVFADVLATQTTLRGLTEALIYD
                     PLTKQVLAAAGLFAGMGVTLPPKQVTDSALNGEVVVLNAGDGTRVQAVVRLDSTPPLM
                     LMIGRPIDPQILEHMTRTEQAVAEYQRLDENRSWLQIAFAWIFAIVAMMVLLAAVLIG
                     LVLANQIARPIGRLINAAERVRSGDLAVRVAEVQTGDELAGLARAFNRMTGQLSAQRG
                     EL"
     gene            complement(1178..2626)
                     /gene="ntrC"
                     /locus_tag="VKQ27_12165"
     CDS             complement(1178..2626)
                     /gene="ntrC"
                     /locus_tag="VKQ27_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HLY89731.1"
                     /translation="MTPPTVLVADDDRSIRTVLTQALGRSGYQVRSTGNAATLWRWVE
                     DGEGDLVITDVVMPDENGLDLIPRIRKLRPDLRVVVMSAQSTLMTAVKATQRGAFEYL
                     PKPFDLQELLSVVGRALAVSPTPAVTAPEPRDADEKLPLIGRSPAMQEIYRTIARLTT
                     ADLTVMINGESGTGKELVARALHDYGKRRTGPFVAINMAAIPRELIESELFGHERGAF
                     TGATTRSQGRFEQANGGTLFLDEIGDMPPEAQTRLLRVLQEGEFTSVGGRQPIKANVR
                     IIAATHRDLRNSIRQGVFREDLFYRLNVVPIRLPPLRERVEDIPLLARHFLERAQDDG
                     LPAKSLDQGAVDQLKKHSWPGNVRELENLMRRLAALCPSETIGAEQIAAELVEAPTAQ
                     TEAPAVAGPEPLVRAVERHIRDFLAAHDGSMSPSDIYDRVIAEVERPLIRLTLAATRG
                     NQIKAAAMLGLNRNTLRKKIRDLDIPVVRGLM"
     gene            complement(2648..3787)
                     /locus_tag="VKQ27_12170"
     CDS             complement(2648..3787)
                     /locus_tag="VKQ27_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY89732.1"
                     /translation="MINPVTRAALALRERRRAPDPGFILGALPVPVVMLDAENRFRYA
                     NPAAEQFLGMSSSGLSQLRLRDLVPDDNPLFLLVNQVRQGVATVSDHDLSMDSPRLNK
                     RGVTVQGSPLPEEPGAVLLVMQDASAARALDRQLAFRSAARSVTGMAAILAHEVKNPL
                     SGIRGAAQLLESSVEPQDRELTVLIRDEADRIRALVDRMEIFGEKPIARSAVNIHRVL
                     EHVRKLAQSGFAPHVRFQEIYDPSLPPVWGNRDQLVQVLLNLVKNATEAATHETQPNP
                     EITLITSFQHGMRLAVPGTTTRMEIPLLVAVRDNGPGIPDDIRPHLFEPFVTSKASGS
                     GLGLALVAKIIGDHGGLIEVDSRPGRTEFRLHLPVLTDEEAEPGR"
     gene            complement(3784..>4506)
                     /locus_tag="VKQ27_12175"
     CDS             complement(3784..>4506)
                     /locus_tag="VKQ27_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HLY89733.1"
                     /translation="ALMRDETAAAAILAATVRAVAVPVTLKMRMGWDHASLNAPRLAR
                     IAQDCGIRMVTVHGRTRQQFYTGSADWDFISQVKDAVEIPVIANGDILTEDDAADALR
                     RSGADGLMIGRGCYGRPWFLAQVAHFLRTGSRLPDPPLAQQKDIITTHYRAVLEQFGE
                     PAGVRLARKHVSWYSRGLPGSAEFRAVVNRLPDAASVLRLINGFYDRLIAGGITRAAP
                     MRTELLAGSGTAEAEGTTRWAA"
BASE COUNT          831 a         1473 c         1442 g          760 t
ORIGIN      
        1 agttcgccgc gctgcgcgga aagctggccg gtcatgcgat tgaacgcccg cgcaaggcca
       61 gcgagttcat cgccggtctg cacctccgcg acacgcaccg ccagatcgcc gcttcgcacc
      121 cgctccgccg cattgatcag ccggccaatg ggacgcgcaa tctgattggc cagcacaaga
      181 ccaatgagca cagccgcgag caggaccatc attgcgacga tggcgaagat ccaggcaaag
      241 gcaatctgta gccaggacct gttttcgtcg aggcgctgat attcggcgac cgcctgttca
      301 gtccgcgtca tatgttcgag gatctgtgga tcgatcggcc ggccaatcat cagcatcagc
      361 ggcggtgtcg aatcaagacg caccaccgct tgcacgcggg ttccgtcgcc tgcgttcagg
      421 accaccacct cgccattgag ggccgaatcc gtgacctgct tcggcggcag cgtgacgccc
      481 ataccggcaa acagaccggc tgcggccaaa acctgcttgg tcagggggtc gtaaatcagt
      541 gcttcggtaa gaccgcgcaa cgtggtctgc gtagcaagga cgtcagcgaa cactgacgga
      601 tcagcactca ggaactgacc ggcgtgtgtc agatcgttgg ccatttcgag cgcaaccgaa
      661 cggatgttgt cgcggtgttc ctcgaggtag ccgcgcgaaa cctgcagcga ctcattgatc
      721 gcctgacgga cgggatcgtt gaaccaggac tgaatgccga agtggaagaa cgccaccgcg
      781 aagcacgcga ccacgatggc cggcgcgacc gccacgccac cgaacagcag cacgagccgc
      841 acatgcagcc gcgagccggc ggaacctcgc cggcgctcga cccaaaccct cgtcagccgg
      901 ccggcaagga ccgcgacgag cagtaggatc gcgatcaggt tcgccaggac gaggccaacg
      961 gtgatgcctg gcttcgggct cagcggtgca ccgcgcgcca ggatgatgaa cgtcgcaagg
     1021 gcaacgccga acgccagtgt ggccaacacc agcgtgacga ccttgccgag caggatattg
     1081 gcgatgttgc ctaccaacgc gcgcgtacgg gagacctcaa cgtccggcgg caggtcgacc
     1141 ggttcaggcc tcagtgaccc gggtggcggg gcgacgttca catcagccct cgcacgactg
     1201 gaatgtcgag atcacggatt ttcttgcgca gggtgttccg gttgaggccg agcatggccg
     1261 ctgccttgat ctgattgcca cgcgttgccg cgagcgtaag gcggatcagc ggccgctcca
     1321 cctcggcgat cacccggtcg tatatgtcgg aaggcgacat gctgccgtcg tgtgccgcga
     1381 ggaaatcccg gatgtgacgt tcgacggcac gcaccagcgg ctcagggccg gcaactgcgg
     1441 gcgcctcggt ctgcgcggtg ggcgcctcca ccagctccgc cgcaatctgt tcggcaccga
     1501 tggtttcgct gggacatagc gcagccaggc gccgcatgag gttctccagc tcccggacgt
     1561 tccccggcca actgtgcttc ttgagctggt ccaccgcgcc ctggtccaga cttttcgcgg
     1621 gaaggccatc atcctgggcc cgctcgagga agtgccgcgc aaggagtggg atgtcctcta
     1681 cccgctcccg cagaggcggt agccgaatcg gcacaacgtt caaccggtag aacaggtcct
     1741 cgcggaacac cccttgcctg attgagtttc gcagatcgcg gtgcgtggcc gcgatgatgc
     1801 gcacattcgc cttgatcggc tggcggccac ccaccgatgt aaactcgcct tcctggagca
     1861 cacgcagcag gcgcgtctgc gcctcgggcg gcatgtcgcc aatctcatcg aggaagagcg
     1921 tgccgccatt ggcctgttcg aaacggccct ggctgcgcgt ggtggcgccc gtgaaggcac
     1981 cccgctcgtg gccgaacagc tcactctcga tcaattcccg ggggatcgcc gccatgttga
     2041 tggcgacgaa agggcctgtt cggcgcttgc cgtagtcatg cagtgcgcgg gcaacgagct
     2101 ccttcccagt gcccgattcg ccattgatca tgacggtcag gtcggccgtc gtgaggcggg
     2161 caatggtgcg gtagatttcc tgcatcgccg ggctgcggcc gatcagcggc aacttctcgt
     2221 cagcatcacg cggctctggc gccgtgaccg cgggtgtcgg cgagaccgcc aacgcacggc
     2281 cgacaacgga cagaagttcc tgcaaatcga acggcttggg caaatattcg aatgcaccgc
     2341 gctgggtcgc ctttaccgcc gtcatcagcg tggactgcgc gctcatgacc accacgcgca
     2401 ggtcgggccg gagcttgcgg atacgcggga tcaggtccag gccattctca tccggcatca
     2461 ccacgtcagt gatcacaagg tcgccctcgc cgtcctcgac ccagcgccat agggtcgcag
     2521 cattgccggt gctgcgcacc tgataacccg agcggcccaa tgcctgggtc agcacggtcc
     2581 gaatcgagcg gtcgtcgtcg gcgaccagga cagtaggagg agtcatcata agtccttagc
     2641 catcgcttca gcggccgggc tcggcttcct cgtccgtcag caccggaaga tggaggcgaa
     2701 attcggtgcg gccggggcgg ctgtccactt cgatcagtcc gccatggtcg ccaatgatct
     2761 tcgccaccaa tgccaggccg agcccgctgc cactggcctt ggaggtgaca aacggttcga
     2821 acaggtgcgg ccggatgtcg tccggaatgc cgggaccatt gtcacgcacc gctacgagca
     2881 aggggatttc catccgggtc gtggtgccgg gcaccgccag gcgcataccg tgctggaagc
     2941 tggtgatcag cgtgatctcc gggttgggct gcgtctcatg cgtcgccgcc tcggtcgcgt
     3001 tcttcaccag gttcagcaag acctggacga gctggtcgcg gttgccccac accggcggca
     3061 gcgacggatc gtagatttcc tggaaccgca catgtggcgc gaacccggac tgcgcaagct
     3121 tgcgcacgtg ctccagcacc cgatggatat taacggcgct gcgggcaatc ggcttctcgc
     3181 caaaaatctc catgcgatcg acgagggcac gtatcctgtc agcctcgtcg cggatcagca
     3241 cggtgagttc ccggtcctga ggctcgacgc tggattccag caattgagct gccccgcgga
     3301 taccggagag cgggttcttc acctcatgtg cgaggattgc cgccatgccc gtgacgctgc
     3361 gggcagcgct gcgaaacgca agctgccgat cgagcgcgcg ggccgccgat gcatcctgca
     3421 taaccagcag cacagcgccc ggttcttcag gcagcggcga gccctgaacg gtaacccctc
     3481 gtttgttcaa tcggggactg tccatcgaaa gatcatgatc ggagacggtc gcgacgccct
     3541 gacggacctg attgaccagc aggaacaaag gattgtcgtc cggtaccaga tcgcgcaggc
     3601 gaagctgcga caggccggag gaggacatgc cgagaaactg ttcggctgcg ggatttgcgt
     3661 agcggaaccg gttttccgca tcgagcatga ccacagggac tggcagcgcg cccagaatga
     3721 acccgggatc tggcgcccgg cggcgctcac gcagcgccaa agccgcacgc gtcaccggat
     3781 tgatcatgcg gcccaccggg tcgttccttc ggcctccgcc gtgccagacc ccgccagcaa
     3841 ctcggtccgc attggcgccg ctctggtgat cccaccagcg atcaggcgat cgtagaaacc
     3901 attgatcaac cgcagtaccg acgcagcgtc cgggagccgg ttcaccacgg cacggaattc
     3961 agcggaaccg ggtagacccc gcgagtacca ggagacgtgc ttgcgcgcca ggcgcacgcc
     4021 tgctggttcg ccgaattgct ccagaacggc gcggtaatgc gttgtgataa tgtctttttg
     4081 ctgtgccagc ggtgggtccg gcaagcggct gccggtgcgc aggaaatgcg ccacctgggc
     4141 caggaaccat ggacgtccat agcagccgcg cccgatcatc aggccgtccg caccggagcg
     4201 gcgcagcgca tcggccgcat catcctcggt gaggatgtcg ccattggcga tcaccgggat
     4261 ttccaccgcg tccttcacct ggctgatgaa gtcccagtcc gccgaaccgg tgtagaactg
     4321 ctggcgggtg cggccatgca ccgtgaccat gcggatgccg cagtcctgcg cgatccgtgc
     4381 cagacgtggc gcgttcaggc tggcgtggtc ccagcccatc cgcatcttca gcgtgaccgg
     4441 aacagcgacc gcgcgcaccg tggcggcaag gatcgcggcg gcggccgtct cgtcgcgcat
     4501 cagcgc
//
LOCUS       DATGEM010000383         5816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_512165, whole genome shotgun sequence.
ACCESSION   DATGEM010000383 DATGEM010000000
VERSION     DATGEM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512165"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1113
                     /locus_tag="VKQ27_12180"
     CDS             310..1113
                     /locus_tag="VKQ27_12180"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY89734.1"
                     /translation="MNRLDSKVALISGAARGIGGETARLMAQAGAKVVVGDVLDDRGR
                     QTVAAIAAGGGQAEYIHLDVTREDSWAAAIDVAVSRFGKLDILVNNAGVFVGKGIEEI
                     SLEEWNRLVAVNMTGVFLGTRIAAPALRDAAKSSEHGSAIVNLASIAGIVGSQLDPLY
                     SMTKGGVTLFTKSAALEFGRKGYGIRVNSIHPGVIQTDMGEQTFVARAQRSGTNDTAK
                     IRQTVTDTVPWGRLGMTMDIAKGIVFLASDDACYMNGAGLVVDGGVTAM"
     gene            complement(1132..1968)
                     /locus_tag="VKQ27_12185"
     CDS             complement(1132..1968)
                     /locus_tag="VKQ27_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89735.1"
                     /translation="MKDLSGKTAIVTGAASGIGLGIATALAEQGANVVMADIQKDTVE
                     QAAHTLSGTNKRVMPVRIDVTQEQSVLDALARAEREFGKLHIACNNAGVPMHGTRLVD
                     VPLRDWEFVIGVNMWGVIHGIRHFVPAILKHGEEGHIVNTASVAGVQNRRGTNQGPYS
                     MTKYAVLSLSEALEIELEGTNVGVSVLCPGPIATNIARGARNRPDHMGGPQVRATDEA
                     TLSERLATTGLDPKRVGERVVDAIRTGTFYAFVSAVPADVIRARHRRIEEALNSEWVT
                     HY"
     gene            complement(1965..3038)
                     /locus_tag="VKQ27_12190"
     CDS             complement(1965..3038)
                     /locus_tag="VKQ27_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY89736.1"
                     /translation="MARVGESKGEYRGGVYREDGALKSWLEKRPREAALEPDLPIIDP
                     HHHFWDNPHRGLYLLPELLADLGGGHNIVSTVFLECRAMYRKAGPREMAPLGEVEFVA
                     GLAAMSASGNYGPCRVAEGIVSGGDLTVGAKVRELFEAQKVAAGGRLRGMRHGVAWDT
                     HEAVYKYASREVPLHQVMDPKFREGFAQLAPLGMSFESWQYHPQLPDATDLARSFPGT
                     TIILNHVGGILGVGPYNNHRQEVLQGWHKDISALAKCPNVYCKLGGLGMVSVGYDFHE
                     QELPPTSEDLAAAWRPYIEHCIEAFGVDRCMFESNFPPDKQSGGYTELWNAFKRITAN
                     ATPAEKKALYSGTAAKVYRLIAP"
     gene            3156..4262
                     /locus_tag="VKQ27_12195"
     CDS             3156..4262
                     /locus_tag="VKQ27_12195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89737.1"
                     /translation="MQFSFTSEQEEFRAILRRFLEDKSPATVVRRLMETEAGWDREAW
                     REMNEQLGLSAVHIPEAYGGQGFGFVELGIVLEEMGRALLCAPFFASTVLAATAIMNA
                     GTEAQKRRLLPGIASGETIATLAFTEPNGRWDADGIETRVSGAYRLDGVKSFVVDGHT
                     ADLIVVVARGEKGLSFFTVAGDAPGLTRRPLKVMDPTRKLALLEFRGVAAELLGEDGG
                     GDAPFARTMVQAAVCLANESVGGAERLREQALEYANLRVQFGRAISSFQSMKHKQADM
                     LCDVELAKSAAYVAASAAAEDDAELPALASLAKATACEAYMQTAIHTIQIHGGIGFTW
                     DNDTHLWFKRAKSSEVFLGDPTYHRELMMRAWGV"
     gene            4265..5521
                     /locus_tag="VKQ27_12200"
     CDS             4265..5521
                     /locus_tag="VKQ27_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89738.1"
                     /translation="MSEATEASVRADVRAWLEANFDPELGLVEWRNKLIDSGWGVPSW
                     PKAWFGRELPMALEMVVEDEMQRVGAVGVAKTGIRRLAAATLLVHGTDDQKQRFLRRS
                     LTGEDTWCQLFSEPGSGSDLAGAMTRAEFKGNRWVINGQKVWTTSAHHADWGLLLART
                     DWDKPKHQGLSYFVLDIHQPGVEVRPLRQMNGHASFNQVFFTDAEVPPENLVNEVGRG
                     WAVATTTLMHERRGADGISRWGKSSDRAGRVYEEERAENATVMEPYKWYPQRAGRVDL
                     VIERAKAAGRASDPVVRQEIARLLCMSKSAEWTARRARAAQMQGKPQGPEGSLGKLVS
                     SNVARAAARVHTLIAGADAMLTGEDGAMNGVIAEILVSVPAGSIAGGTDEIQRNIISE
                     RVLGMPKEPRTDNDRPFRDVPKNLVV"
     gene            5616..>5816
                     /locus_tag="VKQ27_12205"
     CDS             5616..>5816
                     /locus_tag="VKQ27_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HLY89739.1"
                     /translation="MQLASRTYPVDDIHAAQELYHANGWTDGLPVVPPTAEAVAACLD
                     WAMMAPDQLVGIEPVRGVAITAE"
BASE COUNT          948 a         1604 c         2225 g         1039 t
ORIGIN      
        1 gcctcgatca gcaggcggga accgccggga acacgaagca attccgcgat cgcgccgctg
       61 ccgccgccgg taatcgccag tgcggccttg cgtccggacg cctgggttgc ggcgatcaat
      121 tcctgccacg cagagaccgg cgggctcatg ttgtgtcttg tggtccgacg ttgaagttcg
      181 aagcagggag cgtgagaggc agttcaggcg gagtcaacca aagcgttgca ccccggtggc
      241 tcgccggtgc tcgtgcggtt gcgcctgatg tcataggctc gccgaagagt ggaagcggag
      301 accagcgata tgaatcgtct cgatagcaag gttgctctga tctcgggggc cgcgcgtggc
      361 atcggtggag agacggcgcg actgatggcg caggccggcg cgaaggtggt ggtaggcgac
      421 gtgctggacg atcgcggacg gcagacagtc gctgccatcg cggccggcgg cgggcaggct
      481 gagtatatac atctggatgt gacgcgggag gactcgtggg ctgccgcgat cgacgttgcg
      541 gtcagccggt ttggcaagct cgacatcctg gtgaacaacg ccggcgtctt cgtgggcaag
      601 gggatcgagg agatcagcct ggaagaatgg aaccgactgg ttgccgtgaa catgaccggc
      661 gtgttcctgg gcaccaggat tgccgctccc gcactccgcg atgcggcgaa atcgagcgag
      721 cacggcagtg ccatcgtcaa tcttgcctcg atcgccggta tcgtcggctc acagcttgat
      781 ccgttgtatt cgatgacgaa ggggggcgta acgctgttca ccaaatcggc ggcgctggag
      841 ttcgggcgaa aagggtatgg gatccgggtg aattcgatcc atcctggcgt gattcagacc
      901 gacatgggtg agcagacttt cgtcgcgcgt gcgcagcggt ccggtacgaa cgatactgcg
      961 aagatccggc agactgtgac tgacactgtg ccgtggggac ggctgggcat gacgatggat
     1021 atcgccaagg gcatcgtttt cctggcctcg gatgatgcct gctacatgaa tggcgccggg
     1081 cttgtcgttg atggcggcgt tacagcgatg tgagggcgag cgggtggcgc gtcagtagtg
     1141 cgtcacccac tccgagttca gtgcctcctc gatgcggcgg tggcgcgccc tgatcacgtc
     1201 ggcggggacg gcgctgacga aggcgtagaa ggtcccggtg cggatcgcat cgaccacacg
     1261 ctcgccgacg cgcttcggat cgaggccggt ggttgcgagg cgctcgctca gcgtcgcttc
     1321 atcggtggcg cggacctgcg ggccgcccat gtggtcgggg cggttgcgtg caccgcgcgc
     1381 gatgttggtg gcgatcgggc cggggcacag gacggagacg ccgacattgg tgccttccag
     1441 ttcgatctcc agcgcctcgg acagcgacag gactgcgtat ttcgtcatcg aatacgggcc
     1501 ctggttggtg ccgcggcggt tctggacgcc ggcgaccgag gcggtgttga cgatatggcc
     1561 ttcctcgccg tgcttcagga tggcgggcac gaagtggcgg atgccatgga tcacgcccca
     1621 catgttcacg ccgatgacga actcccagtc gcgcagcggc acgtcgacca ggcgggtacc
     1681 atgcatcggc acaccggcgt tgttgcaggc gatgtggagc ttgccgaact cgcgctcggc
     1741 ccgcgccagg gcatccagca ctgactgttc ctgcgtcacg tcgatgcgca ccggcatgac
     1801 gcgcttgttg gtgccggaga gcgtgtgggc ggcctgctct accgtgtctt tctggatgtc
     1861 ggccatcacc acgttagcgc cctgctcggc gagcgcggtg gcgatgccga ggccgatgcc
     1921 ggaggcagca ccagtgacga tggcggtctt gccggagagg tccttcatgg cgcgatcagc
     1981 cgatagactt tcgcggcggt gccgctgtag agcgccttct tctcggcggg tgttgcgttg
     2041 gcggtgatgc gcttgaaggc gttccacagc tcggtgtagc cgcctgactg cttatcgggc
     2101 gggaagttgc tttcgaacat gcagcggtcg acgccgaacg cttcgatgca gtgttcgata
     2161 tatgggcgcc aggcggccgc caggtcttcg gaggttggcg gcagctcttg ctcatggaag
     2221 tcgtagccca ctgagaccat gccgaggccg cctagcttgc aatagacgtt ggggcacttg
     2281 gcgagtgcgc tgatgtcctt gtgccagcct tgcaggactt cttggcggtg gttgttgtag
     2341 gggccgacgc cgaggatgcc gccgacgtgg ttcaggatga tcgtggtgcc ggggaacgag
     2401 cgcgcgaggt cggtcgcgtc gggtagttgc ggatgatact gccacgattc gaagctcatc
     2461 ccgagcgggg cgagctgcgc gaagccttcg cggaatttcg gatccatgac ctggtgcagc
     2521 gggacctcgc gggaggcgta tttgtaaacg gcctcgtggg tgtcccaggc gacgccgtgg
     2581 cgcatgccgc gcaaccggcc gccggcggcg accttctgtg cctcgaagag ttcgcggacc
     2641 ttggcgccaa cggtgaggtc gccaccgctg acgatgcctt cggcgacgcg gcaagggccg
     2701 tagttgcccg aggcgctcat tgcggcgagg ccggcgacga attcgacctc gccaagcggg
     2761 gccatctcgc gcgggccggc tttgcggtac atcgcacggc actcgaggaa gaccgtggag
     2821 acgatgttgt gaccgccgcc gagatcggcg agcagttcgg gcagcagata gagaccgcga
     2881 tgcgggttgt cccagaagtg gtggtgcggg tcgatgatgg gaaggtcggg ctccagcgct
     2941 gcctcgcgcg ggcgcttttc gagccaggat ttcagggcgc cgtcctcgcg ataaacgccg
     3001 ccgcggtact cgcctttcga ctcaccaaca cgtgccattg ccgtttcctc cgtcgcgagg
     3061 acggtagagc ggggtggtgg gttgggcaac cggcgggcct tgcggaagta gcgctgctgg
     3121 cttcaatagg gtgtgccgtc ccgggaggaa accgcatgca gttcagcttc accagcgagc
     3181 aggaagaatt tcgcgccatc ctgcgtcggt tcctggagga caagtcgccg gccaccgtgg
     3241 tgcggcggtt gatggagacc gaggcggggt gggatcgcga ggcttggcgc gagatgaacg
     3301 agcagcttgg gttgagtgcg gtgcatatcc cggaggccta tggcggacag gggttcgggt
     3361 tcgtcgaact ggggatcgtg ctggaggaga tgggccgcgc actgctgtgc gcgccgttct
     3421 ttgcgtccac ggtgctggcg gcgacggcga tcatgaatgc gggcaccgag gcgcagaagc
     3481 ggaggctgct gccggggatc gcctcgggcg agacgatcgc gacgctggcg ttcaccgaac
     3541 cgaacggtcg gtgggacgcg gacgggatcg agacgcgcgt atcaggggcg tatcggctgg
     3601 atggggtgaa gagtttcgtc gtcgatgggc atacggcgga cctgattgtc gtggtcgcgc
     3661 gcggggagaa ggggctgtcg ttcttcaccg tggcgggtga tgcgccgggg ctgacgcggc
     3721 gtccgctgaa ggtaatggat ccgacgcgca agctcgcttt gctggagttt cgtggggtcg
     3781 cggcggagct gttgggtgag gatggcggcg gggacgcgcc gttcgcccgc acgatggtcc
     3841 aggcggcggt ttgtctggcg aacgagagcg tgggcggcgc cgaacggctg cgggagcagg
     3901 cgctggagta cgccaatctg cgggtgcagt tcgggcgggc gatctcatcg ttccagtcga
     3961 tgaagcacaa gcaggcggac atgctgtgcg acgtggaact ggcgaagtcg gcggcctatg
     4021 tcgcggcgtc cgcggcggcg gaggatgatg ccgagctgcc ggcgctggca tcgctggcga
     4081 aggcgacggc gtgcgaggcg tatatgcaga cggcgataca tacgatccag atccatggcg
     4141 ggatcggctt cacgtgggac aacgacacac atctgtggtt caagcgggcg aagagctccg
     4201 aggtgttcct gggtgatccg acgtatcacc gggagctcat gatgcgggcg tggggggtct
     4261 gacgatgagc gaagcgaccg aagcctctgt ccgcgccgat gtgcgtgcct ggctggaggc
     4321 gaatttcgat ccggagcttg ggttggtgga gtggcgcaac aagctgatcg actccgggtg
     4381 gggcgtgccg tcctggccga aggcgtggtt cggtcgcgag ctgccgatgg cgctggagat
     4441 ggtggtcgag gatgagatgc agcgggtcgg cgccgttggg gtggcgaaga ccggcatccg
     4501 ccggctggcc gcggcgacgc tgctggtgca tggcacagac gatcagaagc agcggttcct
     4561 gcggcgcagc ctgacgggcg aggatacgtg gtgccagttg ttctcggagc cggggtcggg
     4621 gtctgatctt gccggcgcga tgacgcgggc ggagttcaag ggcaatcgtt gggtgatcaa
     4681 cgggcagaag gtgtggacca ccagcgcgca ccatgccgat tgggggctgc tgcttgcacg
     4741 gactgattgg gacaagccta agcaccaggg gctgtcgtat ttcgttttgg atatacacca
     4801 gccgggcgtg gaggtgcggc cgctgcggca gatgaacggg catgccagct tcaaccaggt
     4861 gttcttcacg gatgccgagg tgccgccgga gaacctggtg aacgaagtgg gccggggctg
     4921 ggcggtagcg accacgacgc tgatgcatga gcggcgtggc gcggatggga tcagccgctg
     4981 ggggaagtcg tccgatcggg cggggcgggt gtacgaggaa gagcgggccg agaacgccac
     5041 cgtgatggag ccatacaagt ggtatccaca gcgggccggg cgggttgatc tcgtgatcga
     5101 gcgggcgaag gcggctgggc gtgcgagcga tccggtggtg cggcaggaga tcgccaggct
     5161 gctgtgcatg tcgaagtcgg cggagtggac tgcgcggcga gcacgggcgg cgcagatgca
     5221 gggcaagcct caggggccag aagggtcgct cggcaagcta gtgtcgagca atgtggcgcg
     5281 cgcggcggcg cgggtgcata cgctgatcgc cggcgcggac gcgatgctga ccggcgagga
     5341 tggcgcgatg aacggggtga tcgcggagat cctggtcagt gtgccggcgg ggtcgatcgc
     5401 gggcgggacg gacgagatcc agcggaacat catctcggag cgcgtgctgg ggatgccgaa
     5461 ggagccgcgc acggacaatg atcggccgtt ccgggatgtg ccgaagaatt tggtggtgtg
     5521 agcatgtctc cgtccggcct gcactgtcat tgcgagcgaa gcgaagcaac cccgtcgaga
     5581 ttgcttcgct tcgctcgcaa tgacaggtga agccgatgca gctcgcctcg cgcacctacc
     5641 cggtcgatga catccatgcg gcgcaggaac tttaccacgc caatggctgg accgatggct
     5701 tgccggtcgt gccccccacc gcggaggccg tggcagcatg cctggattgg gcgatgatgg
     5761 ccccggatca gcttgtcggc atcgagccgg tgcgcggcgt ggcgatcacc gcggag
//
LOCUS       DATGEM010000384        10842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1261614, whole genome shotgun sequence.
ACCESSION   DATGEM010000384 DATGEM010000000
VERSION     DATGEM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261614"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..7252
                     /locus_tag="VKQ27_12210"
     CDS             <1..7252
                     /locus_tag="VKQ27_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89740.1"
                     /translation="GRPSRWSRERYLHPTPRTRGRSYTFDHGLIANVSEFDVELFGIS
                     PREAQQIDPQQLLLLEVTYEALEDAGLSRTVLAGSKVGVFVGASFCDHFHRRLMDVAT
                     LDGYFMTGAAMSILSNRISHAFDWKGPSMTVDTACSSSLVALHLACQALRVGEVDTAI
                     VGAVNLLLAPEPFVGFSQAALLSPSGRCRPFSAEADGYVRSEGAAAIVLRRSDALAPD
                     NAPRAWVLGTGSNSDGHTNGISLPNSGTQAALLREVYAAAGVAADDLCYLEAHATGTA
                     VGDPIEARAIGETLGCSRVRPLPVGSAKSSFGHLEAASGMVGLFAAMTVVQSRSLPRL
                     AHFAAPNPNIDFAGLCLAPTAVARPLADHEAAIVGVNSFGFGGTNAHAIIAAEPLAPV
                     TPRHRRAAPATAELPPLILSAHSAPALAALADRWVEFLPTVHQAELVPTLRGAARGRP
                     LYDHRLVAHGSEPQALTEALRSWRAGSPTSRVISGQADADALGFVFAGNGSQWPGMGQ
                     LAMAENRTFRVAIGRLDGILGLRLGWSIVAALEVPDPARLRRTDVAQPLLFAIQYALV
                     EALAEAGIRPAAVVGHSVGEIGAAWACGALGLEDALDVLIARSSLQQRSHGLGRMAVC
                     GLDSAALHATLAEHGLALEIAAMNAPRAVTVAGSAADIARLGTVMRAADIFFSELDLD
                     YAFHSSLLDPIEQELRHRLAGLVSSPAVVPFVSTVTGEVESAPALDAEYWWRNIREPV
                     RLDAALDALDALGVGTLLEIGPHPVLQFFLRQGQRKSGRPIRVLASLARGQTSEDPVL
                     GVIAACHVAGQDIGRGPLFDGERRTRELPRYPFQRQTISLASSIENLDVSDIARTHPL
                     LGFPIGDSGLLWRAQVDTTLFPWLGDHVVDGDVIIPATALLDMALAAARVRFADNQVL
                     DLLDVEFRQPVLLEAGSLVELGLQISPERNTFEIVSRTRLSDAPWRLHVSGRLAVGEW
                     APAGTPLSPLAGVTMAPDVLYDRAASVGLHYGTTFRTVETLTLHDTRSATVTLRVPDA
                     DHGLRALSGSILDPAWVDGSLHGLIGLLTSRNGTDPAMMLPRRFARLRHFGSSKAVHT
                     ARLTIVRTGPRAVEAAIQLLDADGERVADMSGVWLARVPLRRQHEALPLWATELRSLP
                     PLRRPMSRLRAPLTGPALGNTASQDAAILLDACISSAAYAALAPLAAVAELNPVALVA
                     AGDVHPAARRLLQHLLQWLEADGLASHHGGAWRLAEQCDLPAAPEIWRSLLGDLPQIS
                     GELALLGAAIEGLPRALANGSHAAADLSRAMLEQMLKDGPDGHAIIAAVAGRVAGIAN
                     ACSVDRPLRILEIGARDASLARTIARLLGRSVPGLYYVVAIAGIEAADLPDNVLHGLP
                     GRFEAWDQVSEPPPEILPGAFDLIIGTYAGSLGPAGRALPTILAPLLAPEGRMILAEP
                     LPNRLWQLALDGDLERSGGTGQHLLSGASWLALMDGAGLRHAHAEQIAGMIWPASLLE
                     GEAPLFDEPTETSDRQTAMRQTDVLLVGEPEDALTQALIQQLGTQGQPPRCCRLPELL
                     AELERPASDIPASDIIVITGDTATASRQLGNLAGAIGRIAKPARLCLVTRGDATGEPV
                     AAALEAALRVLANENNGLCCRSIRISRNLLPEVAAMRIVEELQAGADEPELYLSATAR
                     QAPRVRRLSPATLPIESAQLAIGQPGNLDTLHWHPFTATKPGAGEVMLDVAAAGINFR
                     DLMWSLGVLPDEALLDGFAGATLGMECAGTVLEVGADITALQPGDRVMALAPAALATH
                     TTTRAAACVPIPASMSFAAAATMPVAFMTVCHALGDLARITEGETVLVHAAAGGVGLA
                     AIQYATARGAEVIVTAGSEVKRTFLRTLGIRHVFDSRSLRFADDVMAATSSRGVDVVL
                     NSLHGEAMRRSMALLAPFGRFIELGKRDFIADTAVGVRPLRRNAAYFGVDTDHLAHLR
                     PARAAAMLEELSALMASGALRPLPHRVMGYADVVEAFRVMQSAEHIGKLVLSVERAAP
                     RVAPRPTGFAAEPAGAYVVSGGVNGFGLETAKWLAARGARHLALVGRRGGKTPGADEA
                     LAGLRTAGCDAQVYACDVSDATALATVLATVRERQAPIVGVVHAAMVLRDGMMSGMAE
                     ADFDAVMAPKFAGANELDRLTRNDPISLFLLFSSATTLLANPGQANYVAANAAIEALA
                     ERRRAEGLPALAVAWGPIADVGVLARDAGAADVLARRFGNQKTTARTALDQLDRMLAS
                     PQCVAAPVSVNWRQARQTLPGLASPRFSEIAARSREATVETGVALRELLLQKTSAEAR
                     AMVQAVLTDIIGEVLRLPAGRLQPQARLADIGIDSLINVEVRLAIEERFGTNLPMVAI
                     SDETTLGDMAEIVLRAIGVGNQEAPNLALRLAERNEGQGAWLAGVEGSNAAD"
     gene            7269..8624
                     /locus_tag="VKQ27_12215"
     CDS             7269..8624
                     /locus_tag="VKQ27_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY89741.1"
                     /translation="MAGIFGISDDAKRKLINGMLARRTSSAAPPPARTTERPPLGDPQ
                     RYDELRLMRAAAAHLDLANPYFKVHEGTAGATACIGNRQYINFASYNYCGLNGDPRVQ
                     QAAIRAMRTFGTSAGASRLVAGERPLHAELEQALAAHYTAEDCVVMVSGHATNVTVLG
                     HLLSAGDLVLHDALAQNSVVQGCVLSGAHRLPFPHNDLHAAERLLERARPTARQALIV
                     VEGHYGMDGDVPDLAGLVALARRYDAHIMVDEAHSLGVLGATGRGVFEHCGVAPSDID
                     IWMGTLSKTLAGCGGYIAGSAAMVDYLRHTAPGFVYSVGISPPLAAASLAALRALHEE
                     PERVARLQANGALFLAEAKARSLDTGASIGAAIVPVVVGSSIRAARAAELLFERGVNV
                     QPIIHPAVPERAARLRFFLSYQHEPEQIREAVRLTAESVAQAKAEQIPLADVIRLLSP
                     R"
     gene            8633..9394
                     /locus_tag="VKQ27_12220"
     CDS             8633..9394
                     /locus_tag="VKQ27_12220"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89742.1"
                     /translation="MLRQHIATIEALVVRDLMVRFGRQDLGFVWAVLEPMILVSGVLG
                     VWSVLHGHDIRGVPVLSFIVTGYMPLTVWRHTTSSMTRVMQNQSNLLYHRPITQFDIV
                     FSRLFLELLSATTALLVVGFILISLGLVRPVADWSLALLGWSLTCLYFGGMGMVIAGL
                     TQVWEKTEKFVQPVNYFALPFSGVFFMVDWLPSWAQNAVLWNPSVNCIEMLRAGVIGP
                     RIATHFDAGYIILVTLAMLVLGLSFVRASRRHIEV"
     gene            complement(9412..10620)
                     /locus_tag="VKQ27_12225"
     CDS             complement(9412..10620)
                     /locus_tag="VKQ27_12225"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLY89743.1"
                     /translation="MRRLEAVEAKLDRLLLQIEFVRTHSATYLGQGLALTHLVNERPM
                     IVNSNDLGGPMQFISGGEYEVENIGVLFSFMSDDTVTFVDAGANLGFFSLMVGERLRP
                     NGKVFAFEPHPLLADLARRNAFINGLESVVTVYPFGLSDVTAETNFGYPKDHLGGGRI
                     GETDQLSRYDVVRSSIRRLDDVLDADVMVDLMKIDVEGHEPQVMRGMQQVIARSPRLK
                     ILFENLGKHADRSARTEEALRPFGFHLYAVVGDAQLSPLAPGELANFSGYVLAARPEQ
                     VATLDRRCFNIYPSQLIIDGTPPRRPGELLINEGTPAEALFHGPYWFLRSGVWALHIH
                     GKIEGEIELLIAERYGRVTLKRRMSAAQMQWQFFNDQNLVQFEVVARAAPDIDKARVE
                     IERIELVRMG"
BASE COUNT         1763 a         3444 c         3564 g         2071 t
ORIGIN      
        1 cggccgccca tcgcgctggt cgcgggagcg ctatctgcac ccaaccccgc ggacgcgggg
       61 gcgtagctac acgttcgatc acgggctgat cgccaacgtc agcgagttcg atgtcgaact
      121 cttcggcatt tcgccgcgtg aggcccagca aatcgatccg cagcagctcc ttctgctgga
      181 ggtgacctac gaggcgctgg aagacgccgg actgtcacga accgtgctcg ccggcagcaa
      241 ggtcggtgtg ttcgtaggcg cgtccttttg cgaccacttc catcgccggt tgatggatgt
      301 cgccacgctc gacggttact tcatgaccgg cgccgcgatg agcatcctgt cgaaccgcat
      361 ctcgcatgca ttcgactgga aggggccgag catgaccgtc gatacggcgt gctcgtcctc
      421 gctcgtggcg ttgcacctgg cctgccaggc gctgcgggtt ggcgaggtcg ataccgccat
      481 cgtcggcgcg gtaaatctct tgctcgcgcc agagccgttt gtcggctttt cccaggccgc
      541 attgctgtca ccgagcgggc gctgccggcc attttcggca gaggctgacg gttacgtccg
      601 ctccgagggt gccgcagcca tcgtcctgcg ccggagcgac gcgttggcac cagacaacgc
      661 gccgcgcgcc tgggtgctgg ggaccggctc caattccgac ggtcatacca acgggatttc
      721 gctgccgaac tcagggacgc aggctgccct gctgcgcgag gtctatgccg cggccggcgt
      781 cgcggcggat gatctctgct atcttgaggc gcacgcgacc ggtactgccg tcggcgatcc
      841 gatcgaggcg cgcgccatag gcgaaacctt gggttgttcg cgcgtcaggc cgctgcctgt
      901 cggatcggcg aaatccagct tcggccacct ggaggcggca agcggaatgg ttggcctgtt
      961 cgccgccatg actgtggtcc agtcacgctc gctgcctcgt ctggcgcatt ttgccgcgcc
     1021 aaacccgaac atcgacttcg caggactttg cctggcgccg actgccgtcg ctcggcctct
     1081 ggcggaccat gaagcagcca tcgtcggcgt aaattctttc ggcttcggcg gcacgaatgc
     1141 ccacgcgatc attgctgccg aacccctcgc gccagtaacc ccgcgccatc gtcgtgccgc
     1201 cccggccacc gctgagttgc caccgctgat tctgtcagct cacagtgctc cggcgctcgc
     1261 agcacttgcc gaccgctggg tggaattcct gccgacggtg catcaggcgg agctggtgcc
     1321 gacgttgcgc ggtgcggcac gggggcggcc gctgtatgat catcgcctcg tggcgcatgg
     1381 atcggagccc caggcactca ccgaggcatt gcggtcgtgg cgcgctggtt cgccgacatc
     1441 gcgggtgatc agcggccagg ctgacgccga cgctcttggc ttcgtcttcg ccggcaacgg
     1501 gtcacaatgg cctggcatgg ggcagctcgc gatggccgag aaccgcacgt tccgtgtggc
     1561 catcggccgg ctcgatggca tcctcggcct tcggctgggt tggtcgatcg tagcagcact
     1621 ggaagtgcca gacccggcaa ggctgcggcg gaccgatgtt gcccagcctc tgctgttcgc
     1681 catccaatac gcgcttgtcg aggctttggc cgaagccggc attcgccctg cggcggtggt
     1741 gggccacagc gttggcgaga ttggcgccgc ctgggcgtgc ggcgccctcg gcctggaaga
     1801 cgcgctcgat gtgctgatcg cccgcagctc gctgcagcag cgcagtcacg gcctggggcg
     1861 catggcagtg tgcggactgg atagcgccgc gctgcacgcc acgctcgcgg aacacggtct
     1921 ggcgctggaa atcgctgcga tgaatgcgcc gcgtgccgtc accgtggcag gttccgccgc
     1981 cgatattgcc cgccttggca ccgtcatgcg cgcggcggac atcttcttca gcgaactcga
     2041 cctggattac gcattccaca gcagcctgct ggatccaatc gagcaggaat tgcggcacag
     2101 gctcgccggc ctggtcagca gcccggctgt cgtgccgttt gtatctacag tgaccggcga
     2161 ggtcgagtcc gcgcctgcgc tggatgcgga atactggtgg cgcaacatcc gtgagccggt
     2221 ccgcctggat gcggcgctcg atgcgctcga tgcgctcggt gtcggcacgc tgctggagat
     2281 tggcccgcac cccgtgttgc agttcttcct tcgccagggg cagcgcaagt cggggcgccc
     2341 gatccgcgtg cttgcaagtc tcgcgcgggg tcagaccagc gaggacccgg tcctgggcgt
     2401 gatcgctgcc tgtcacgttg ccggccagga catcgggcgt ggtccgctgt ttgacggtga
     2461 acgacgaacc cgcgaattgc cgcgctatcc gttccagcgg cagacgatct cattggccag
     2521 ttcgattgag aaccttgatg tatcggatat cgctcgaacg catcctctgc tgggttttcc
     2581 gatcggcgac agcggactgc tctggcgcgc gcaggtagat acaacgctct tcccctggct
     2641 cggggatcat gtggtcgacg gcgatgtcat cattcctgcg acagcgctcc tcgacatggc
     2701 acttgccgcg gcacgtgtcc gcttcgctga caaccaggta cttgacttgc tggacgttga
     2761 gtttcgccag ccggtgctgc tcgaagcggg cagcctcgtc gagcttggcc tgcagatcag
     2821 ccccgagcgc aacacgttcg aaatcgtcag ccggacgcgg ctgtccgatg ctccgtggcg
     2881 gttgcatgtc agtggtcgtc tcgctgtcgg cgagtgggcg ccggcgggga ctccattgtc
     2941 tcccctcgcg ggcgtcacga tggcaccaga cgtcctctac gaccgtgcgg catcggtcgg
     3001 attgcattac ggcacgacgt tccggactgt cgaaacgctg acgctgcacg acacgcgatc
     3061 ggcaacggtt acgcttcgtg tgccggacgc cgatcacggc ttgcgcgccc tgtcgggatc
     3121 catcctggat cctgcctggg tcgatggttc cttgcatgga ctgatcgggc tgctgaccag
     3181 tcggaacggt acggatccgg ccatgatgct gccccggcgg ttcgcccggc tccgccattt
     3241 cggttcatcg aaggccgtcc atacagcgcg gctgacgatt gtccgaaccg gacctcgagc
     3301 cgttgaagcc gccattcaat tgctggatgc cgatggcgag cgggtggccg acatgtctgg
     3361 tgtctggttg gcgcgggtgc ccttgcgtcg tcagcacgaa gcattgcccc tgtgggccac
     3421 agagctgcgt tcgctgccgc cgctgcgccg gccgatgagc cggctgcgcg cgccgttgac
     3481 aggccccgcg ctgggcaaca ccgcgtctca ggacgcagcc attctcctgg atgcgtgcat
     3541 cagctccgct gcctacgccg cgcttgcgcc cttggcggca gtcgccgaat tgaacccggt
     3601 tgcactggtg gcggcgggag atgtgcatcc cgcggccaga cggctgttgc aacatctgct
     3661 gcaatggctc gaggcggatg ggctggcaag ccaccacggt ggcgcctggc ggctcgccga
     3721 gcagtgcgat ctgccggcgg cacccgagat ctggcggtcc ctgctcggcg atttgccgca
     3781 gatttccggc gaactcgcgc tcctcggcgc cgcgatagag ggattgccgc gcgccctcgc
     3841 aaatggctcg catgctgccg cggacctttc gcgagccatg ctcgagcaga tgctcaagga
     3901 tggtccggat ggccatgcaa ttatcgccgc cgtcgctggc cgcgttgcag gcatcgcaaa
     3961 cgcatgttcc gtcgaccgtc cattgcgcat tctggagatc ggcgcgcgcg atgcgagtct
     4021 ggctcgcaca atcgctcgcc tgctcgggcg gtcggtgcct ggcctctact acgtcgtcgc
     4081 gatcgccgga atcgaggcgg cggatctgcc cgacaatgtc ctgcatggat tgccgggtcg
     4141 gttcgaggcc tgggatcagg tgagcgagcc gccgccggaa atccttcctg gagccttcga
     4201 cctgatcatc ggcacctatg cgggtagcct ggggccggcc ggccgagccc tgccgacaat
     4261 actcgccccg ctgctggcac cggaaggccg aatgatcctg gccgagccgc tgccgaaccg
     4321 gctgtggcaa ctcgccttgg acggcgatct ggaacgttcc ggtggcacag gtcagcatct
     4381 gctgagcggt gcgagttggc tggcattgat ggatggcgcc ggcctgcgcc atgcccatgc
     4441 ggagcagatt gccgggatga tctggcccgc gtcgctgctc gaaggcgagg caccgctgtt
     4501 cgatgagccc accgagacat cggatcggca aacggcgatg cgccagaccg atgtcctgtt
     4561 ggtcggagag ccggaggacg cgctcacgca ggctctgatc cagcaactgg gcacgcaagg
     4621 ccagccaccg cgatgctgcc gtttgcccga gttgctcgcc gaactggagc ggccagcgtc
     4681 ggacatccca gcgtcggata tcatcgtcat caccggcgat acagcgaccg cgtcgcggca
     4741 gttgggcaat cttgccggcg cgatcggccg cattgcgaaa cctgccaggc tgtgcctcgt
     4801 gacgcgtggc gatgcgaccg gcgaaccggt cgccgccgcg ctggaagctg ccctgcgcgt
     4861 gctggcaaac gagaacaacg gcctgtgctg ccgcagcatc cgcatctcgc gcaacctgtt
     4921 gccggaagtc gcagccatgc ggattgtcga ggaactgcag gcaggcgccg acgaaccgga
     4981 actttacctg tccgccaccg cacggcaggc cccacgggtc aggcggctct cgccggcaac
     5041 gttgccaatt gaatcagcgc aattggcaat cggccagccg ggcaatctcg atacgttgca
     5101 ttggcatccc tttaccgcga ccaagcccgg agctggcgaa gtgatgctgg acgttgccgc
     5161 ggccgggatc aacttccgcg atctgatgtg gtcgctcggt gtcttgcccg atgaagcgct
     5221 gctggacggc tttgccgggg ccacactcgg gatggaatgc gcgggcaccg ttctggaggt
     5281 tggggcagac atcaccgcgt tgcagcccgg cgatcgggtg atggctctgg cgccggcggc
     5341 gctcgcaacg cacacgacga cacgcgctgc cgcctgcgtg ccgatacccg cgagcatgag
     5401 ctttgccgcc gccgcgacga tgccggttgc cttcatgact gtctgccatg cgctcggcga
     5461 tctggcccgg atcaccgaag gtgagacggt actggtgcac gccgctgccg gcggcgtcgg
     5521 ccttgctgcc atccagtatg cgaccgcgcg aggcgcggag gtcatcgtca cggcgggatc
     5581 cgaggtgaag cggaccttcc tccggacgct gggtatccgg cacgtgttcg attcgcgcag
     5641 cctgcgtttc gccgatgacg tcatggcggc aaccagcagt cgtggggtcg atgtcgtgct
     5701 gaactcgctg cacggcgaag cgatgcgccg cagtatggca cttcttgctc cattcggccg
     5761 cttcatcgag ctgggcaagc gcgacttcat cgccgacacg gctgtcggtg tgcgcccgtt
     5821 gcggcgcaat gctgcctatt tcggcgtaga tacagaccat cttgcgcacc tgcggcccgc
     5881 gcgtgcggca gcgatgctcg aggaactgtc cgcattgatg gcgagcggtg cgttgcgtcc
     5941 gctgccacat cgcgtgatgg gctacgccga tgtggtcgag gccttccgtg tgatgcaatc
     6001 cgccgagcat atcggcaagc tggtgctgtc ggtggagcgc gccgcaccgc gtgtcgcgcc
     6061 gcggccgact ggtttcgccg ccgaaccagc gggagcctac gtggtctcgg gcggagtgaa
     6121 cggcttcggc ctggaaacag cgaaatggct ggcagcgcgg ggtgccaggc atctcgcgct
     6181 ggttggaaga cgcggtggca agaccccagg tgccgacgag gcattggccg gtttgcgcac
     6241 tgcgggctgc gatgcccagg tctatgcctg tgatgtctct gatgccactg cgctcgcgac
     6301 cgtcctggca acagtgcgcg agcggcaggc tcccatcgtc ggcgtggtgc atgcggcgat
     6361 ggtgcttcgc gacggcatga tgtccgggat ggcggaggcc gacttcgacg cggtgatggc
     6421 acccaagttc gcaggtgcca acgaactcga tcggttgacg cgcaatgatc cgatatcgct
     6481 gttcctgctg ttctcctcgg ccacgacgct gctcgccaat cccgggcagg ccaattacgt
     6541 cgcggccaac gctgcaatcg aagcacttgc cgaacggcgt cgtgccgaag gattgccggc
     6601 actggccgtc gcctgggggc cgatcgccga tgtaggcgtg ctcgcgcgcg atgctggcgc
     6661 tgccgacgtg ctggcgcgcc gcttcggcaa ccagaagacg acggcgcgta cagcgctcga
     6721 ccagttggat cggatgctgg cgtcgccgca gtgtgttgcg gcgccagtct cggtgaactg
     6781 gcggcaggca cggcagacac ttccggggct cgcatcgccg cgcttctccg agattgcggc
     6841 acgatcgcgc gaagctacgg tcgagaccgg tgtcgcattg cgcgaactcc tgttgcagaa
     6901 gacttcagcg gaggcacgcg ccatggtgca ggcggtgctg accgacatca tcggcgaggt
     6961 gcttcgcctg cctgccgggc gcctccaacc acaggcccgc ctcgcggata tcggcatcga
     7021 ctcgttgatc aatgtcgagg tgcggctcgc gatcgaagag cgctttggaa cgaacctgcc
     7081 gatggtcgcc atctccgacg agacgacgct cggcgatatg gcagaaatcg tgctgcgggc
     7141 gatcggtgtg ggcaatcagg aagcgccgaa tctcgccctg cggctggcgg aacgcaacga
     7201 gggtcaggga gcctggctcg ccggggtgga aggcagcaac gctgcggatt gattgagcgg
     7261 gaggtatggt ggcagggata tttggcatat cggacgatgc gaagcggaag ctgatcaacg
     7321 gtatgcttgc gcgtcgcacg tcctccgccg caccaccgcc ggcccggacg accgagcggc
     7381 ctccgctcgg cgacccacag cgctatgacg agctgcgtct gatgcgcgcc gcggccgcgc
     7441 atctcgacct ggccaatccc tacttcaaag tgcacgaagg cacagcaggc gccacagcct
     7501 gtatcggtaa tcgtcaatac atcaactttg cctcgtataa ttactgcggg ctcaacggtg
     7561 atcctcgcgt gcagcaggcg gcgatccgcg caatgaggac gttcgggacc tcggctggcg
     7621 cgagccggct tgtggccggt gagcgcccgt tgcacgccga gctggaacag gcattggcgg
     7681 cacactacac ggcggaggac tgcgtggtga tggtcagcgg ccacgctacc aatgtcacgg
     7741 tactcggcca tctgttatcg gccggcgatc tggtgctgca tgacgcgctg gctcagaaca
     7801 gcgtcgtgca aggctgcgtt ctgtccggtg cgcaccggct gccattcccg cacaacgatc
     7861 tgcatgcggc ggaacgattg ctggaacggg cgaggccgac ggcgcgccag gcgcttatcg
     7921 tcgtggaagg ccactacggt atggacggcg acgtccccga cctggcgggc ctcgtcgcac
     7981 tcgcgcgacg ctacgatgcg catatcatgg tcgatgaggc gcacagcctg ggtgtcctgg
     8041 gcgccaccgg gcgcggcgtg ttcgagcact gtggcgttgc tccatccgat atcgacatct
     8101 ggatgggcac actcagcaag acgctggccg gttgcggcgg ctatatcgcc ggaagtgccg
     8161 cgatggtcga ttacctgcgc catacagcgc ccgggttcgt ctactcggtg ggcatatcgc
     8221 cgccgctggc cgcggcatcg ctggcggcgt tgcgggcctt gcacgaggag ccggaacgtg
     8281 tcgcacggct gcaggcgaat ggagcgttgt tcctggccga agccaaggcg cgctccctgg
     8341 ataccggcgc gtcgatcggt gcggcaattg tgccggttgt cgtaggcagt tcgatccgcg
     8401 cagcgcgtgc cgctgagttg ttgttcgagc gtggcgtcaa tgtccagcct atcatccacc
     8461 cggctgtccc ggagcgggcg gcgaggctgc gcttcttcct gtcgtatcaa cacgaaccgg
     8521 agcagatccg cgaagcggtg cggctgaccg ccgaatcagt cgcccaggcg aaggcggagc
     8581 aaatcccgct cgccgacgtt atccgtttgc tgtcgccacg ttagggaagc cggtgctgcg
     8641 tcaacacatt gccaccatcg aggcgctggt tgtccgtgac ctgatggtgc gcttcggacg
     8701 tcaggatctt ggttttgtct gggcagtcct ggaaccgatg attctggtca gcggcgtgct
     8761 tggcgtatgg agcgtgctgc acggccatga catccgcggg gtgccggtgc tgagcttcat
     8821 tgttaccggc tacatgccgc tgaccgtatg gcggcacacg acaagttcca tgacccgcgt
     8881 catgcagaat caatcgaacc tgctatatca tcggccgatc acgcagttcg acatcgtctt
     8941 ttcccgactt ttcctcgaac tgctgtcagc gaccaccgca ttgctggtcg tgggatttat
     9001 tctgatatcc ctgggactcg tccggcccgt cgccgactgg agcctggcgc tgctcggctg
     9061 gtcgctgacg tgtctttatt ttggtggcat gggcatggtc attgccggac tgactcaggt
     9121 ctgggagaag accgagaaat tcgtgcagcc ggtgaactac tttgcgttgc cgttctccgg
     9181 cgtctttttc atggtcgact ggctgccgag ctgggcgcag aatgcggtgc tgtggaatcc
     9241 ttcggtgaat tgcatcgaga tgctgcgggc tggcgttatt ggcccccgga tcgccacgca
     9301 tttcgacgct ggctacatta tactggtgac actcgcgatg ctggtgctcg gactctcatt
     9361 cgtccgcgcg agcaggcgcc acatcgaggt gtagcaagag aatgcgcaag actatcccat
     9421 gcgcaccagt tcaatgcgct cgatctcgac ccgcgcctta tcgatgtccg gggctgcccg
     9481 agcgacgact tcgaactgaa ccaggttctg gtcgttgaag aactgccatt gcatctgcgc
     9541 agcagacatc cgccgtttca gcgtgacccg gccataccgc tctgcgataa gcaattcgat
     9601 ctcgccctcg atcttgccat ggatatgcaa agcccatacc ccgctacgca gaaaccaata
     9661 ggggccatga aacaaggcct ccgcgggtgt tccttcgttg atcagtaatt cgccgggacg
     9721 gcgcggtggc gtgccgtcaa ttatgagctg tgatggatag atattgaaac agcgccgatc
     9781 cagggtagcc acctgctcgg ggcgcgcagc gaggacgtag ccggagaagt tcgccagctc
     9841 gccaggagca agcggagaca gctgagcatc accgacgacg gcatagagat ggaagccgaa
     9901 tggccgcaat gcctcttcgg tcctggctga acgatccgca tgcttgccaa ggttctcgaa
     9961 caggatcttg agccgcggcg agcgtgcgat gacctgctgc atgccgcgca tgacctgtgg
    10021 ctcgtgccct tcgacatcga tcttcatcag gtccaccatg acatcggcat cgagcacgtc
    10081 gtccagacga cgtatgctgg accggacgac atcgtaacgg ctcagctgat ctgtctcgcc
    10141 gatccggcct ccacccagat ggtccttggg atatccgaag ttcgtttctg ccgtgacgtc
    10201 cgacaggccg aacggatata cagtgacgac ggactccagc ccgttgatga atgcattgcg
    10261 ccgcgcgagg tccgcgagta agggatgcgg ctcgaacgcg aaaaccttgc cattcggccg
    10321 aagccgctca ccgaccatga gcgagaaaaa cccgaggttg gcacccgcat cgacgaacgt
    10381 gacggtatcg tcgctcatga agctgaacaa caccccgata ttctcaactt catactcgcc
    10441 gcccgagatg aactgcatcg ggccaccgag gtcattggag ttgactatca ttggccgttc
    10501 attgaccaga tgcgtaagcg caagcccttg accaaggtat gtcgccgagt gtgtgcggac
    10561 aaattcgatt tgcagcagca gccggtcgag tttcgcctcg acggcctcga gcctcctcac
    10621 caactcatcc atatgtgcgg gctctccgga tcggtcgttc cccctacgcc aggcgtacca
    10681 gttcgatgcg ctctatcgcg acccgggctt tggcactgaa aggccgggca atgacttcga
    10741 aatgggtcag gttgcgttcg ttggtgaatt gccactgcat ctgtgcggtg gacatccgct
    10801 ggtccaggac cactttgcca tagcgctctg ccaggaaaat ct
//
LOCUS       DATGEM010000385         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_89384, whole genome shotgun sequence.
ACCESSION   DATGEM010000385 DATGEM010000000
VERSION     DATGEM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89384"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VKQ27_12230"
     CDS             <1..1065
                     /locus_tag="VKQ27_12230"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HLY89744.1"
                     /translation="RLHMTLLAVLRHLTFAAALALLSAAVVRGMIAVHVLDRPDPRKA
                     HTIATPKGGGVGIIVAFLVGIAVLYRFAEFSRLADPYFIGVIEASVAIAVVSFLDDLY
                     DWKFTVKLAAQVLATLVAVGSGIYVSDYRVPYVGPLYVGWLGAPATVLWLLFVTNAMN
                     FIDGLNGLAAGVALIVSCFLCAIAGLYGGWFAYFASLLLAAGLAGFLPFNFPRARIFM
                     GDVGSQFCGFILAILAVAASRLDGVELSFLIVPMLLSGVLFDVAFTLVRRAVAGERVT
                     APHRGHLYQVAQRSGVPDVAVALIYWGFAGLGGICAFVFLAAPSPDKPFVPLALLLPQ
                     LIWLGFVVRQAHVARLGRWG"
     gene            complement(1137..1532)
                     /locus_tag="VKQ27_12235"
     CDS             complement(1137..1532)
                     /locus_tag="VKQ27_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY89745.1"
                     /translation="MAVNPTADQMVGILRETVVALVRRDGPDLSARQLGVFLTCYLRD
                     GGHTVRGLAAELNVSKPAITRALDRLGELDLARRKVDPLDRRSILVQRTLKGAAFLRD
                     LRSIMGEAATASKKAPAATEAHSRRTATA"
     gene            complement(1679..3079)
                     /locus_tag="VKQ27_12240"
     CDS             complement(1679..3079)
                     /locus_tag="VKQ27_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HLY89746.1"
                     /translation="MLEQALNCLVATAATMRPLHAVRPNGLAVFLETLSEPQSRFLRQ
                     LEFKAAAQELVFLPGESGVVGAVLGLGDDRSPAAFGNLAFRLPNGDPWQLQPGDYDLP
                     AATLGFCLGAYRYGSLKTSENRPSAQLVAPQEQTASRIAASATWMVRDLVNTPANLLG
                     PVELADFAISLGKRYGAATELIADAALNSDYPTVAMVGQGSARPPRVAILRWTGSTAH
                     AAAPLISLCGKGVCFDTGGYDMKPSSGMLRMKKDMGGAATVLGLARMIMEADLPVRLT
                     VRVGCVENSVSGTAMRPLDVVRTRKGITVEIGNTDAEGRLVLCDLLADACDESPALLI
                     DCATLTGAARVALGPDLPAMFCNDDGWADTLLHAGQAEHDPLWRLPLWAGYDRWLDSP
                     IADCGNVSSKPHAGAITAALFLQRFVSAGTVWAHFDLYAWNDQSGPARPEGGEAYAMR
                     AVFAGIRQVSADPQSH"
     gene            complement(3150..>4046)
                     /locus_tag="VKQ27_12245"
     CDS             complement(3150..>4046)
                     /locus_tag="VKQ27_12245"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HLY89747.1"
                     /translation="NRVIPVNAMHITEVQQLAKQRPIDVAFIAVKSYDTVWATRMIRQ
                     YLSPGGFIVSSQNCINEERIASVVGWGRTVGCVVGGGVGVELFEPGHVRRTMMKNPNV
                     VSFHVGEPHGRVTKRTEQLAEMLHGVDNADATDNLWGERWTKLCVNGMRNGVSAATGL
                     GGNARDGHDAIRRICIRLGGESVRVGQALGFTLGKIGALDPEKLALASEGNRGALEEI
                     ETLMLAGTSAAARSDLQRPSMAQDMAKGRRTEIEFMNGFIAEKGDEVGVPAPTHAMLT
                     DVVKRVERGEIAADPTNVLVNT"
BASE COUNT          624 a         1243 c         1368 g          811 t
ORIGIN      
        1 cggctgcaca tgaccctgct tgccgtcctg cgccatctga cattcgccgc cgcgctggcg
       61 ctgctttccg ccgccgtggt gcgggggatg atcgccgtgc acgtcctgga tcggcctgac
      121 ccgcgcaaag cgcatacgat cgctacgccg aaaggtgggg gcgttggcat tatcgtcgcc
      181 ttcctggtgg gcatcgcggt gctttaccgc ttcgcggaat tctcgcgcct ggctgatccg
      241 tatttcatcg gcgtgatcga ggcctcggtg gcgatcgcgg tggtgtcgtt cctggacgac
      301 ctctacgact ggaaattcac ggtgaagctg gcggcacagg tgctggcaac gctggtggcg
      361 gtcggcagcg gaatctacgt ctcggactat cgtgtgccgt atgtcgggcc gctttacgtg
      421 ggctggctgg gagctccggc gaccgtcctg tggctgctgt ttgtgaccaa cgcgatgaac
      481 ttcatcgacg ggctgaacgg attggccgcg ggtgttgcgc tcatcgtgtc atgcttcctg
      541 tgcgccatcg cgggtctgta tggtggctgg ttcgcctatt tcgcatcgtt gctgctggcg
      601 gctggcctgg cggggttcct gccgttcaat tttcctcgtg cgcgcatttt catgggggat
      661 gtcggcagcc agttctgcgg tttcattctg gctattcttg cagtcgcggc cagccggctg
      721 gacggggttg agctgtcctt cctgatcgtg ccgatgctgc tgtctggcgt gctgttcgac
      781 gtggcgttca cgctggtgcg gcgggcggtt gccggcgaga gggtgaccgc gccgcatcga
      841 gggcacctgt atcaggtagc acagcgatcc ggtgtgccgg acgtcgccgt cgcgctgatc
      901 tactggggtt tcgcggggtt gggcggcatc tgtgccttcg tgttccttgc cgcgccttcc
      961 ccggataagc cgttcgttcc gctggcgctg ttgctgccgc aacttatctg gctcggcttc
     1021 gtcgttcgcc aggcacatgt ggcgaggttg ggccggtggg gctgagtggc gctgaccgaa
     1081 ccgctctgcc cgccctcacg ctttgacgtg gggcggggga ggggcgccag cagggctcag
     1141 gccgtggcgg tgcggcggct atgggcttcc gtggctgcag gcgctttctt ggaggcggtg
     1201 gcggcctcgc ccatgatcga gcgcagatcg cggaggaagg cagcgccctt cagcgtgcgc
     1261 tgaaccagaa tgctgcggcg atccagcggg tccaccttgc ggcgcgcgag atcgagttcg
     1321 cccagccgat cgagcgcgcg ggttatcgcc ggcttcgata cgttcagctc ggcggcgagg
     1381 ccgcggaccg tgtggccgcc gtcgcgcaga tagcaagtga ggaatacgcc aagctggcgc
     1441 gccgaaagat cgggcccgtc gcggcgcacg agtgcgacga cggtctcccg caaaatgcca
     1501 accatttggt cagcggtcgg attcacggcc atgcttattt cctttctggt acccggaccg
     1561 gcaatgcatc cgcgatgggt tcgtggacgc aggggttgct ggtgcgaatt cgatacctag
     1621 tcaatcccat tacaggtgcg aagccttttc ccactcgctc gcgtgcatgt gacagggatt
     1681 aatgcgattg cggatcggcc gatacctgtc tgataccggc gaaaaccgct cgcatggcgt
     1741 aagcttcgcc accttccggg cgagccggtc cggactggtc attccacgca tagaggtcga
     1801 agtgcgccca tacagttcca gccgaaacga aacgctgcaa aaataatgct gctgtgatgg
     1861 cacctgcatg tggctttgac gagacattgc cgcaatcggc aatagggctg tcaagccagc
     1921 ggtcgtagcc agcccacaat ggcaggcgcc aaagcggatc atgttcggct tggccggcat
     1981 gcaacagtgt gtcggcccag ccatcatcgt tgcagaacat ggctggcagg tctgggccga
     2041 gtgccacgcg cgccgcaccg gtcagtgtgg cgcagtcgat cagcagggcc ggtgattcgt
     2101 cgcatgcatc ggccagcagg tcacacaaga cgagccgccc ctcggcatct gtattgccga
     2161 tttcgactgt tattcctttg cgtgtgcgta cgacgtcgag cgggcgcatc gcggtgccgg
     2221 agacggagtt ttcgacgcaa ccaacccgga ccgtgaggcg cacgggcaga tccgcctcca
     2281 tgatcatacg ggccaggccg agcacggtgg ccgcgcctcc catgtctttc ttcatgcgca
     2341 gcatgccgct cgatggtttc atgtcgtaac caccggtgtc gaagcagacg cctttgccgc
     2401 acagcgaaat caacggcgca gcggcgtgcg cggtgctgcc ggtccatcgc aggatggcga
     2461 cgcgtggcgg ccgcgccgag ccttggccca ccatcgcgac ggtgggataa tcgctgttca
     2521 gcgccgcgtc cgcgatcagt tcggtagcgg caccataacg cttgccgagc gaaatcgcga
     2581 agtccgccaa ttctacgggg cccagcagat tggccggtgt gttgacgagg tcgcgcacca
     2641 tccaggtggc ggatgcggcg atgcgactcg cggtttgttc ttgcggcgca accagctgcg
     2701 cggacggacg gttctccgac gtcttcagcg aaccgtagcg ataggcgccg aggcagaagc
     2761 cgagcgtcgc ggcaggcaga tcgtagtctc caggctgcaa ttgccaggga tcgccgtttg
     2821 gcaggcggaa tgcgaggttg ccgaaggcgg cgggcgagcg atcatcgccc aggccgagca
     2881 cggcgccgac gacaccggat tcgccgggaa ggaacaccaa ttcctgtgct gcggccttga
     2941 attcgagctg gcgcaggaag cgtgactgcg gctcggacag ggtttccagg aaaaccgcaa
     3001 gtccattggg gcgaaccgca tgcaacggtc gcatcgtcgc cgcggtggcc accaggcagt
     3061 tgagcgcttg ttccagcatg gtgcttttga cccttaccgc ttgattagcc ggtgcgggag
     3121 cggccgacct gacgaacgct ggtggctctt catgtgttga ccagcacgtt cgtcgggtcg
     3181 gcggcgattt cgccgcgttc gacgcgtttc acgacatcag tgagcatcgc gtgggttggc
     3241 gcgggcacgc caacttcgtc gcctttctca gcgatgaagc cgttcatgaa ctcaatctcg
     3301 gtgcggcggc cttttgccat gtcctgcgcc atcgagggtc gctgcaggtc gctgcgcgcg
     3361 gcggcgctgg tgccggcgag catcagcgtc tcgatctcct caagcgcgcc gcggttgcct
     3421 tccgaggcca gtgccagttt ctcggggtcc agcgcaccga tcttgcccaa cgtgaagccg
     3481 agcgcctgac cgacgcgcac cgactcgccg cccagcctga tgcagatgcg acggatcgca
     3541 tcgtggccgt cgcgcgcgtt gccgccgagg ccggtggcgg cggaaacgcc attgcgcatg
     3601 ccgttgacgc agagcttggt ccagcgttcg ccccacagat tgtcggtggc gtccgcgttg
     3661 tccacgccgt ggagcatctc ggccagttgt tcggtgcgtt tcgtgacacg gccatgcggt
     3721 tcgccgacgt ggaacgatac gacattgggg ttcttcatca tggtgcggcg gacgtggccg
     3781 ggctcgaaca gctccacgcc gacgccgccg ccgaccacgc atcccacggt gcgcccccag
     3841 ccgacgacgg aggcgatgcg ctcctcgttg atgcagttct gcgacgacac gatgaagccg
     3901 cctggcgaga gatactggcg gatcatccgc gtcgcccaca ccgtgtcgta cgacttcacc
     3961 gcgatgaagg cgacatcgat cgggcgctgt ttggcgagct gctgcacctc ggttatgtgc
     4021 atcgcgttga ccgggatcac acggtt
//
LOCUS       DATGEM010000386         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1026619, whole genome shotgun sequence.
ACCESSION   DATGEM010000386 DATGEM010000000
VERSION     DATGEM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026619"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VKQ27_12250"
     rRNA            <1..315
                     /locus_tag="VKQ27_12250"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            473..587
                     /gene="rrf"
                     /locus_tag="VKQ27_12255"
     rRNA            473..587
                     /gene="rrf"
                     /locus_tag="VKQ27_12255"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            706..782
                     /locus_tag="VKQ27_12260"
     tRNA            706..782
                     /locus_tag="VKQ27_12260"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:740..742,aa:Met,seq:cat)
     gene            complement(846..1337)
                     /locus_tag="VKQ27_12265"
     CDS             complement(846..1337)
                     /locus_tag="VKQ27_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89748.1"
                     /translation="MPLAVTIIYKAGVYCVFAFIVRMIESNAEGWRRGTSIAEFLATF
                     SWHRFLAIQIWLFVLFLIFVSSSELTALLGVGALRQLLFHHRALDLALGRRARTQVLV
                     QLGHLTASHTPEELADRASPANAKLPRLLRGLTKQPVGLREAGKFHSRQGPSSRADGG
                     PNN"
     gene            1411..1758
                     /locus_tag="VKQ27_12270"
     CDS             1411..1758
                     /locus_tag="VKQ27_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HLY89749.1"
                     /translation="MLVTAFRHKDFGVTVGCGMGGGMTEIIDDVVFSRAPLDRDGAQD
                     LLPRLRTLPRLPSLLSGCQPALAADFIAGFTVLVANAPWSGFTLEANPLKVGQDAVAA
                     VDGLLIVESQLSP"
     gene            1900..>2704
                     /locus_tag="VKQ27_12275"
     CDS             1900..>2704
                     /locus_tag="VKQ27_12275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89750.1"
                     /translation="MNFDFSDDSKLLREQAQRFLGEQCTPKVVRHVLEGDAPYSSALW
                     QAMAEMGWLGAAIPDRYEGAGLGYEALCVLAEELGRVLAPVPFVSTVCLAAEAILAAG
                     SEAQKREYLPKIVRGELIATFALAEGPGEPREPGVRARLTDGRITGSKWPAPDGSIAQ
                     LAVVTTRDESGVVLALVDLAAPGVTRSAVPMVDPSADHARLDFDNAPAAVLGTGGHGW
                     DMVRRLLDRAAILTAFAQVGGATASLEMATAYAKERFAFGRPIGSFQAIK"
BASE COUNT          515 a          841 c          873 g          475 t
ORIGIN      
        1 acgtgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggtgaagg
       61 agacttgaga ggatctgtcc ctagtacgag aggaccggga tggacgcacc tctggtgcac
      121 cggttgtcgc gccagcggca ccgccgggta gctatgtgcg gacgggataa ccgctgaaag
      181 catctaagcg ggaaacccac ctcgaaacga ggtctccccg agggccgtga tagaccatca
      241 cgttgatagg ccaggtgtgg aaacgcggca acgcgcgaag ctgactggtc ctaatcgccc
      301 gatagagctc aagatctgtt cccggggtca caagcccagg gacaccatgc atggaaatca
      361 tcctcagcat gtcctcggac ctgatccgaa gaccagcaca ctccggggtg agcccggggc
      421 aagcaacagc atcagaacct gcattcagcc cgataacgga tgggctggac gacctggtgg
      481 ctttggcggg gggcccgcac ccgatcccat cccgaactcg gccgtgaaaa cccccagcgc
      541 ccatggtact gcgtctcaag gcgcgggaga gtaggtcgcc gccaggtctt ccagcccatt
      601 cgttcctcgg gatgagcccg agcacaagta cgaggcccgc ctcacggagt gaggcgctgg
      661 ccgaagtgac gtccccgggc tcgtcccggg gacgtctgtt tctggcgcgg ggtggagcag
      721 cccggtagct cgtcaggctc ataacctgaa ggccgcaggt tcaaatcctg cccccgcaac
      781 caacaatccc ccactccacc aatgccgcta caggtccttg tccgaggtga tctgaccctg
      841 attgttcaat tgttcgggcc accgtcagct cgagatgatg gcccctgtct gctgtgaaat
      901 ttcccggcct cgcgaagacc gaccggctgc ttggtcaatc ctcttagcag gcgcggcagc
      961 ttagcgttgg ctggcgaagc gcgatctgcc agttcctccg gtgtgtggct tgccgtcaga
     1021 tgaccgagtt gcaccagtac ctgagtccgt gctcgccgcc caagcgccaa atccagtgca
     1081 cggtgatgga acagcagctg ccgcagcgcg cccacgccga gcagtgcggt gagctcgcta
     1141 gacgagacaa agataaggaa cagcacgaaa agccagatct ggatcgcgag gaatcggtgc
     1201 cacgagaacg ttgccaagaa ttccgcgatt gaggtaccgc gccgccagcc ttccgcattc
     1261 gattcaatca tccgcacgat aaaggcgaac acgcaataca cgccggcctt gtagatgatc
     1321 gtcacggcca gcggcatcga gtcgtagcgc cgcaggaatg gcgttcgatc agcgccgcgg
     1381 tcaccgcaag tagcagagcc acgcccgctg ttgctggtca ccgcgttccg ccacaaggac
     1441 ttcggcgtca cggtcggctg cggcatgggt ggcggcatga cggagatcat cgacgacgtg
     1501 gtgttctccc gtgcaccgct tgaccgcgat ggagcgcaag atctgctgcc acggttgcgc
     1561 acgctgccgc ggcttccgtc attgctgtcc ggctgccagc cagccctggc cgcggacttc
     1621 atcgccggtt tcactgtatt ggtcgccaat gcgccgtggt caggattcac actcgaggcg
     1681 aatccgttga aggtgggcca ggatgcggtg gcggctgtcg atggcctgct gatcgttgag
     1741 agccagctgt cgccttgagc aacgggtgcc tgagcctgcg ctagcgatcg gagcataggc
     1801 ggcactcgct tccgatgagt ggccgccggg ctcaacagaa ccccgggcac tgttctaaac
     1861 tcgaggagac ttcggacgca gggcatcggg tggagcgcca tgaacttcga tttctccgac
     1921 gacagcaagc tgctgcgcga acaagcgcaa aggtttctgg gcgagcaatg cacgccgaag
     1981 gtggtgcggc acgtattgga aggcgacgcg ccatatagct ccgcgttgtg gcaggcgatg
     2041 gcggaaatgg gctggctcgg cgccgccatt ccggaccgtt acgagggcgc aggcctcggc
     2101 tatgaggcac tgtgcgtgct ggctgaggaa ctcggccgcg tgctggcccc agtgccattt
     2161 gtctcgaccg tctgcctcgc cgccgaagcg atcctggctg ctggcagcga ggcacagaag
     2221 cgcgagtacc tgccgaagat cgtgcgtggc gaactgatag cgacattcgc tctggcggaa
     2281 ggcccaggtg aaccgcgtga accaggtgtg cgggcacgtc ttaccgacgg ccgcatcacg
     2341 ggcagcaagt ggcccgcacc ggatggcagc atcgcacagc tcgccgtggt cacaacgcgc
     2401 gacgagtcgg gcgttgtgct ggcgctggtc gatcttgctg caccgggtgt cacgcgcagc
     2461 gctgtgccga tggtcgatcc tagcgccgat cacgccagac tggatttcga taacgcgcct
     2521 gctgccgtgc tgggcacggg cggccacggc tgggacatgg tgcgacgcct gctggatcgc
     2581 gccgccatcc tgaccgcatt cgcccaggtg ggcggcgcca ctgcatcact cgaaatggcg
     2641 accgcctatg ccaaagaacg cttcgcattc ggccggccga tcggatcgtt ccaggcgatc
     2701 aagc
//
LOCUS       DATGEM010000387         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_576425, whole genome shotgun sequence.
ACCESSION   DATGEM010000387 DATGEM010000000
VERSION     DATGEM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576425"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VKQ27_12280"
     CDS             <1..496
                     /locus_tag="VKQ27_12280"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HLY89751.1"
                     /translation="PRVAPGSDLTALLLDRYLTPADSITVIGLADRWVPMLIERHRIS
                     SIAHYDPPRGFERDPAALSAAVEFVLAHPARFVFLAVGSPRQEILADAVRSTGRATGT
                     GFCIGASLDFLAGARRRAPEWMQRCGLEWLYRLGSDPLHMARRYLLDGPAIFPMLLRE
                     RLHA"
     gene            complement(501..1472)
                     /locus_tag="VKQ27_12285"
     CDS             complement(501..1472)
                     /locus_tag="VKQ27_12285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY89752.1"
                     /translation="MSMRHIAGAPAAPDGAYDADIVILSLDRSEETLAAINSALAQTG
                     VSRHVFVIDQGSRAVGLARLASAIAGRDDTTLVALDRNHGVAGGRNFGSALGHGRIIF
                     GLDNDAEFADSTTLARAVTALDADPQLAAIGCHILLYANGTDDQSSWGYPPRLLPRTS
                     EAFDTITFVGAGHAIRRAAWDMCGGYDDALYFCWEEFDFCLRAIEQGWRIRYRGDIAV
                     RHKVSAEHRIAWSGLRWYHYVRNRMYIGRKWSAGWLPLMPRFGFYLLKGARNGMLMQT
                     LRALPAAIRMARNTDRHPYSARTRAYLRRNDTVYRASPLREIFGHLH"
     gene            complement(1526..2224)
                     /locus_tag="VKQ27_12290"
     CDS             complement(1526..2224)
                     /locus_tag="VKQ27_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLY89753.1"
                     /translation="MFQSSGSTLQPANFGPIPISYGSTPDHHLRSGCNGRTKRAIDVV
                     GAALLITILFVPMLLIAVAIRLDSPGPILFRQRRIGFANLPFETLKFRTMQHRAGRIR
                     TLRQTSRHDPRVTRVGAVLRRTSLDELPQLFNVLRGEMSLVGPRPHAPGTCAGGKPFE
                     LLTPRYPARHEVRPGMTGLAQVRGLRGETETEEKLLRRVEADLEYIENWSLWLDLTIL
                     LRTAKAVFGNQNAY"
     gene            complement(2386..2748)
                     /locus_tag="VKQ27_12295"
     CDS             complement(2386..2748)
                     /locus_tag="VKQ27_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HLY89754.1"
                     /translation="MKFGTVGTVGWIADTVTVYSLRHSLGLYGAGAAAYLVGASMTWL
                     FNRIWTFRGLGAGPVHRQWARFLMVNLVGFTLNRGTYAILVTYSPLCAREPVFATAAG
                     AVAGMFMNFGLSRTMVFR"
     gene            3145..>3764
                     /locus_tag="VKQ27_12300"
     CDS             3145..>3764
                     /locus_tag="VKQ27_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89755.1"
                     /translation="MLAYAARRILATIPVMLLVAFFVFSLLYIAPGDPAAIIAGDQAT
                     PADVERIRISLGLDRPFVVRFGEWLWHILHGDLGTSIFTNLPVSHMIEQRIEPTLSLM
                     LLTIVFAIVVAIPLGVVAAWKQGSWIDSAVMVLAVFGFSTPVFVMGYILAYVFALQLD
                     WLPVQGYTPLSHGVWPWLRNLVLPVVALGAIYVALIARITRASMLEV"
BASE COUNT          678 a         1146 c         1198 g          742 t
ORIGIN      
        1 gccgcgggtc gctcccggca gtgacctgac ggcgctgttg ctggatcgct atcttacccc
       61 ggcggacagt atcaccgtca tcggtctcgc cgaccgctgg gtgccgatgc tgatcgagcg
      121 acaccggatc agttccatcg cgcattacga tccgccgcgt gggttcgagc gggacccggc
      181 cgcgctgtca gctgctgtcg agtttgtcct ggcgcatcct gcgcggttcg ttttcctggc
      241 ggtggggtcg cctcgccagg aaatcctggc agacgcagtc cgcagtactg gacgcgcgac
      301 cggtaccggt ttttgcatcg gggcgagcct ggatttcctc gccggagcac gccggcgtgc
      361 cccggagtgg atgcagcgct gcggcctcga gtggttgtac cggctcggca gtgatccgct
      421 gcatatggcg cgacgctatc tgctcgacgg tcccgccatt ttcccgatgc tgctgcgcga
      481 acgcctgcac gcctgacagg tcagtgcagg tgtccaaaga tttcgcgcag agggctggca
      541 cgatagaccg tatcattccg tcgcaggtaa gcgcgtgtcc ttgcggaata ggggtgccgg
      601 tcagtgttgc gtgccatccg tatcgctgcc ggcaatgcgc gcagagtctg catcagcatt
      661 ccgttgcgcg ctcctttcaa gaggtagaac ccgaagcgcg gcatcagcgg cagccatccc
      721 gcactccact tgcggccgat atacatgcga ttacgcacgt aatggtacca gcggagccct
      781 gaccacgcga tgcggtgctc ggcgctcacc ttgtgccgta ccgcgatatc gccgcgatag
      841 cggatccgcc agccttgctc gatggcgcga agacagaaat cgaattcttc ccagcagaaa
      901 tacagcgcgt catcgtagcc gccgcacatg tcccacgcgg cgcgccggat tgcatgcccg
      961 gccccgacga aggtgatcgt gtcgaaagcc tcgctcgtac gtggcaataa tcgcggcggg
     1021 tagccccacg acgattggtc atccgtgcca ttggcataca gtaagatgtg gcaaccgatc
     1081 gcagccaatt gcggatcggc atcgagcgcc gtcaccgccc gtgcgagtgt cgtgctgtcg
     1141 gcgaattccg catcgttatc caacccgaag atgatccggc catgacccag cgctgagcca
     1201 aagttgcgac caccggcgac tccgtggttc cgatccaatg caaccagggt cgtatcgtca
     1261 cgaccagcga tggcgctggc cagtcgcgcg aggcccacgg cgcgtgagcc ctggtcaatc
     1321 acgaacacat ggcgcgacac gcccgtctgg gccaaggccg agttgatggc ggcgagggtt
     1381 tcctccgatc gatccagcga caggatcacg atatccgcgt cgtaagcgcc gtctggcgcg
     1441 gcaggcgccc cggcaatatg gcgcatactc atacggtgtc gtaacgttct gagcatgatg
     1501 attcggccgg ctggcgccgt cccgctcaat aggcgttctg attcccaaac actgcttttg
     1561 cggtgcgcaa gagaatggtc aggtccagcc agagagacca gttctctata tattccaggt
     1621 cggcttcgac ccttcggagc aatttttcct cagtctcggt ttcgccgcgg agaccgcgca
     1681 cctgcgcaag accggtcata ccgggacgca cttcatgtcg tgcagggtaa cgcggtgtca
     1741 gcagctcgaa tggcttgccg ccggcacaag taccgggcgc atgtgggcgt gggccaacca
     1801 gtgacatctc gccgcgcagg acgttgaaca actgtggcaa ttcgtcgagc gatgttcgtc
     1861 gcaaaacggc acccacccgg gtcacgcgcg gatcgtgccg actggtctgt cgcagcgtcc
     1921 ttatccgccc ggcacgatgc tgcatggtgc ggaacttcaa ggtttcgaag ggaaggttgg
     1981 cgaacccgat ccgccgctgg cggaacagga tcggtccagg gctgtcgaga cggatcgcca
     2041 ctgcgatcag cagcatcgga acgaagagga tggtgatgag cagcgcggcg ccaaccacgt
     2101 cgatggcacg ttttgtccgc ccattgcagc cacttcgaag gtggtggtcc ggcgtgctgc
     2161 cgtaagaaat tgggatcggg ccgaaattgg ccggctgaag cgtactgccg ctgctttgaa
     2221 acatcgccgc gcctgaccag aaccgggaga gttgtatttt tccctaaatg accatcagta
     2281 gttgcaacta tctggcgatc aattcaccgc cggattaaca cggcgcctgc gcctgtgttt
     2341 tggcccaaaa atatttcggt ggggtggcgc ggcggctcga ggcgatcagc gaaacaccat
     2401 cgttctcgag aggccaaaat tcatgaacat gccggcaacc gctccggcgg ctgttgcgaa
     2461 caccggctct cttgcgcaga gcggcgaata ggttaccagg atggcgtagg tgccacggtt
     2521 gagtgtgaag cccacgaggt tgaccataag aaagcgggcc cactgccggt gcacgggccc
     2581 ggctcccagg ccgcgaaagg tccagatccg gttgaacagc cacgtcatgc tggcgcccac
     2641 cagatacgcc gccgcgccgg cgccatagag gccgagcgaa tgtcgcagcg agtaaacggt
     2701 gacggtgtcc gctatccacc ccactgtgcc gacggtgcca aacttcatga actgcaccaa
     2761 cagaaaaatg cgaccggacg tcgcataggg gcgtgtcggt gggaaccggc gggccaatcg
     2821 aagcagcaat gcttgcatgt gccctgacat cgccgcaggg cggcgcgcgg tcaatcctcg
     2881 ccgcacaggg gtgttgcagg ttcaccgccg gcgggggccg acatgtcgtg cagattaagg
     2941 cggcctgaca attgcattag gttacgggca ttagatgcgg atggcggctg gcctttcccc
     3001 ccggcctgag tcagtctagg gatgccgggg ctggaattac ggcaagccag cacaaaacca
     3061 agcggaggcg catgcgggcg atgcggtgtg agtggattgc agggcgtgcg tcgatccgag
     3121 ctttgatggc gtggatctga ctgtatgctc gcttatgctg cgcgccggat cctggcgacc
     3181 attcctgtta tgcttctggt cgccttcttt gtgttcagtc tgctctacat tgcgccaggc
     3241 gaccccgccg cgattatcgc cggtgatcag gcgacgccgg ctgatgtgga gcgcatccgg
     3301 atctcgcttg gcctcgacag accattcgtg gtgaggttcg gcgaatggct gtggcatatc
     3361 ctgcacggtg acctcggcac gtcgatcttc accaacctgc cggtcagcca tatgatcgag
     3421 cagcgtatcg aaccgacttt gtcgctcatg ctgctgacca tcgtattcgc gattgtcgtg
     3481 gccattccgc tgggtgtggt ggcggcctgg aagcagggct cgtggataga cagcgcggtg
     3541 atggtgctgg cggtgttcgg cttttccacg ccggtgttcg tgatggggta tatcctggcc
     3601 tatgtgttcg cgctgcagct cgactggctg ccggtgcagg gctacacgcc gctgtcgcac
     3661 ggcgtgtggc cgtggctgcg caatctcgtt ctccccgtcg tcgcgctcgg cgccatctac
     3721 gtcgcattga tcgcccggat cacccgagcc tcaatgctgg aggt
//
LOCUS       DATGEM010000388         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_384093, whole genome shotgun sequence.
ACCESSION   DATGEM010000388 DATGEM010000000
VERSION     DATGEM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384093"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..288)
                     /locus_tag="VKQ27_12305"
     CDS             complement(58..288)
                     /locus_tag="VKQ27_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89756.1"
                     /translation="MPQSTNAHAAHGLGDFLTTPRLIPIALIAVAIGAVSAFVAAALL
                     RLNGLFTNLFFFQRWGADLVSPAAASILKSRF"
     gene            complement(289..741)
                     /locus_tag="VKQ27_12310"
     CDS             complement(289..741)
                     /locus_tag="VKQ27_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLY89757.1"
                     /translation="MPARLPALTPLDYAALARFRRELRRFLAFSERASHIAGLEPQQH
                     QLLLALRGFGSPDRAPTIGEIAEWLLIQHHSAVELVDRAVARGLVERQQDAHDRRRVL
                     VQLTPAGEAILAQLSLQHREELESTAPALLAALGDVLQPRPQSQARAH"
     gene            836..2584
                     /locus_tag="VKQ27_12315"
     CDS             836..2584
                     /locus_tag="VKQ27_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY89758.1"
                     /translation="MSETTSDVLIDRLCDWGVDTIFGLPGDGINGVMEALRTRQDRIR
                     FVHVRHEEAAAFMACAYAKFTGRLGVCLATSGPGGIHLLNGLYDAKLDRAPVLAITGM
                     AYHDLVHTFTQQDVELDKLFMDVAVYNNRVMGKAHVENTLDLAVRAALAYKGVAHITI
                     PADIQDMQADERSKRNKLGHTSDVRGRAAGVADEAALRQAADLLNSGKKVAILAGQGA
                     LHASTQLEQLADVLAAPIVKPLLGKGAVPDDSPYTTGGIGLLGTRPSQEALEACDRLL
                     MVGTSFPYIEDLPKPGAAAAVQIDIDALRIGLRYPVEVGLVGDSARTIDQLLPLLQRK
                     EDRGFLETAQKGMADWWQQMEEQGTLRSVPMKPQVVGYELNRYLRDDAIITCDSGTIS
                     TWAARHIRIRRGQQFSLSGNLATMANGFPYAIAAQLAYPDRQVVAFVGDGGFSMLMAE
                     LSTCVKYQLPVKIVVIKNNVLGQIKWEQMVFLGNPQYGVELQPMDHVKFAEACGATGF
                     SISDPAECGRIMEQALNTPGPVVIEAVVDPNEPPMPPKVTRDQAMKFGKSLMRGEPNR
                     EKIALTAIADKVRELV"
BASE COUNT          526 a          880 c          913 g          461 t
ORIGIN      
        1 gtccttcggc gccgaagggt ccaccggaac cgatcgaaat tgccgaagag agcggcttca
       61 gaaccgcgac tttaggatcg acgcggcagc tggcgaaacg aggtccgcgc cccagcgctg
      121 gaagaagaac aggttcgtga acaacccgtt gagtcgcagc agggcggcag ccacaaaggc
      181 gctcaccgca ccaattgcca ccgcgatgag cgcgatcgga atcaggcgtg gcgtggtgag
      241 aaagtcgccc agcccatggg cggcgtgcgc gttggttgat tgtggcattc agtgcgcccg
      301 tgcctgactc tgcggacgag gctgcaagac gtcgccgagc gccgcgagca gcgccggtgc
      361 cgtcgactcc agttcctcgc ggtgttggag tgacagctgc gccagtattg cttcgcccgc
      421 cggtgtcaat tggacgagga cgcgccggcg gtcgtgggcg tcctgctggc gctcgaccag
      481 tccacgtgcg acggcgcggt ccacaagctc cacagcgctg tggtgctgga tcaacagcca
      541 ctccgcgatt tcgccgatgg tcggcgcgcg gtcggggctg ccgaagccgc gcaaggcgag
      601 cagcagctgg tgttgctggg gttcgaggcc ggcgatgtgt gaggcgcgct cgctgaaggc
      661 caggaagcgc ctgagctcgc gacggaagcg cgcgagcgcg gcgtagtcga gtggggtgag
      721 ggcgggcagg cgggcgggca agacagcccg attgtatcgt ctgacgacat acttttgcaa
      781 gcctcacggt tcggcgagtc aacggcatgc gttctgcacc gcatcgtgca acgacatgag
      841 cgagacaact tcggatgttc tgatcgaccg cctctgcgat tggggcgtgg ataccatctt
      901 cggcctgccc ggcgatggca tcaacggcgt catggaagcc ctgcgcacac gccaggaccg
      961 cattcgcttc gtacacgtcc gccacgaaga agccgccgcc ttcatggcct gcgcgtacgc
     1021 caagttcacc ggccgcctgg gtgtctgcct cgccacgtcc ggtccaggcg ggatccacct
     1081 gctcaacggg ctgtacgacg ccaagctgga ccgcgcgcca gtgctcgcca tcacaggcat
     1141 ggcgtatcac gacctggtcc acacctttac gcagcaggac gtggagctcg acaagctctt
     1201 catggacgtc gcggtttaca acaaccgcgt catgggtaaa gcccacgtcg agaacacgct
     1261 ggacctcgcc gtgcgcgcgg cgctggccta caaaggcgtt gcgcacatca ccattccagc
     1321 cgacatccag gatatgcagg ccgacgagcg ctccaagcgc aacaagctcg ggcacacctc
     1381 cgacgttcgc ggccgggcag caggtgtcgc cgacgaagcc gcgctacgcc aggcggccga
     1441 cctgctcaac agcggcaaga aagtcgccat cctggccggc cagggcgcgc tgcatgcctc
     1501 cacgcagctc gaacagctcg ccgacgtgct ggccgcgccg atcgtcaagc cgctgctcgg
     1561 caaaggcgcg gtgcccgacg acagccccta caccaccggc ggcatcggac tgctcggcac
     1621 caggccgtcg caggaagcgc tggaggcgtg cgaccggttg ctcatggttg gtacgtcgtt
     1681 cccatatatc gaagacctgc ccaaaccggg agcagccgcc gcggtgcaga tcgacatcga
     1741 tgcgctgcgc atcggcctgc gttaccccgt cgaggttgga ctcgtcggcg acagcgcgcg
     1801 aaccatcgac cagcttttgc cgctgctgca gcgcaaagaa gatcgcggct tcctcgaaac
     1861 cgcgcaaaag ggcatggccg attggtggca gcagatggag gagcaaggaa ccctgcgctc
     1921 cgtccccatg aagccgcaag ttgtcgggta cgaactgaat cgctacttgc gcgacgacgc
     1981 gattatcacc tgcgacagtg gcacaatttc cacgtgggca gcccgccaca tccgcattcg
     2041 gcgcgggcag caattctcgc tcagcggcaa tctcgccacg atggccaacg ggtttccgta
     2101 tgccatcgcc gcgcagctcg cctatcccga ccgccaggtg gttgccttcg tcggcgacgg
     2161 cggcttttcc atgctgatgg cggagctgtc gacctgcgtg aagtatcagc tcccggtcaa
     2221 gatcgtggtg atcaaaaaca acgtgctggg acagatcaag tgggaacaga tggtgtttct
     2281 cggcaatccg caatacggcg tcgaattgca gccgatggat cacgtcaaat tcgccgaagc
     2341 gtgcggtgct accggctttt cgatctcgga tccggcggag tgtggtcgga ttatggaaca
     2401 ggcgctcaac acacctggtc cggtggtgat cgaggcggtg gttgatccaa acgagccgcc
     2461 aatgccgccg aaggtcacgc gcgaccaggc gatgaagttc ggcaagtcgc tgatgcgcgg
     2521 cgaaccaaat cgcgaaaaga tcgcactgac ggcgattgcc gacaaagtgc gcgagctggt
     2581 atgagcgcag tcgcggagtc cgactagcgt ggtctggcgc atgcacagcg aggcaggcga
     2641 tccgcctggc ggagccgttt gtggaatgcg gcgtctcgcg tgtcgacgag gcggtctcgt
     2701 ccgatcaact gcatgaactc gcgctgatcc cgcaagcggg cgccgtcgag atcctgtgtc
     2761 cagcctgatg ctggttcagc
//
LOCUS       DATGEM010000389        18742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_928226, whole genome shotgun sequence.
ACCESSION   DATGEM010000389 DATGEM010000000
VERSION     DATGEM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 18742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18742
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928226"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VKQ27_12320"
     CDS             <1..1032
                     /locus_tag="VKQ27_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HLY89759.1"
                     /translation="GRCNFTNVEAVPDRYLSANPHFAKSALGRYRPEQFIELVDRYGI
                     AWHEKTLGQLFCDGSARQIVAMLLDECAVGDTTIHCGDPIRDIAHADGRFTVTFGGQA
                     ATAPALVIATGGPSIPKMGATGFAYDVARRFGLKVVEPRPALVPLTLGGDEALFRSLS
                     GVASDVVARAGKTSFREAALFTHRGLSGPAILQISSYWRHGENLGIDFLPSAEAGWLR
                     EAKRARPRAGLRSVLGGLLPDRLAETLAERLDLVGELANLSDRALEAAERRLADWRFQ
                     PNGTEGFAKAEVTVGGIATTGLSSQTMEARTVPGLYAIGEAVDVTGWLGGYNFQWAWA
                     SGWAAGQAL"
     gene            complement(1029..3407)
                     /locus_tag="VKQ27_12325"
     CDS             complement(1029..3407)
                     /locus_tag="VKQ27_12325"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HLY89760.1"
                     /translation="MIARLLLALSLLLATPAIAQDADPIATATQSLDQAADEFQAIDG
                     AFNGNLSTTERQSLQDRANTVKQTTTDQVATLTTQLGLIDARIAELGPVTPGVVEAPD
                     IRAQRKLLAQQRSTVDSAIKRGKLLGVEADQLAVEIGESQANAFSERMTERVASPLTP
                     VFWTALLHASPRDGRRVAALLAAEGEALRRGLSGDGLIPALIGLAIAIALAGPIRMAL
                     RATGRRYVIEHVPGSRVRRSGLALWRATVGACIPGLATVALIEGLRGGGMLAPGWEGL
                     AQSFQRAVFVAALITAVAGALLQPKQGSWRLVGLSDHAAKALLPWAVAAAGAVLTSVL
                     LIAFRDAIGASGPSRAAADSAAALVYIALVLGLLLTIGRLRSHRTRADEEHETSQAGF
                     AFVSLAAWAVVIIAVVAMSAGYVSLAYFLSRFVVWVAVVASTLYLSLLAVDDICSNLF
                     RRDSRLADNLHHGFGLRRSLIDQFGVALSALLRLVLIAIAASLIFAPFGSNLTTLLAQ
                     SDRIAQGVTIGQITIAPGAIFRAGAVLFVGLFVVRSAQHWLTNRYLPATELDAGARNS
                     ISMVARYTGLILAVLWSLASLGIGMERIALLLSALSVGIGFGLQAITQNFVSGLILLA
                     ERPVKIGDLVRIGDQEGDVKRISVRATEIQIADRSTLIVPNSELITKTIRNMTLADPI
                     GRVQLNFSVPMGTDIGQVREMVLRMFAETHAVLNDPAPSLFIDSIIDGRINLMSFGYV
                     HSPREVYATRSHLLFELLEKLPAVGIELGTAPQRFEFIGAPPAPAAKKPEKS"
     gene            complement(3489..3893)
                     /gene="cyoD"
                     /locus_tag="VKQ27_12330"
     CDS             complement(3489..3893)
                     /gene="cyoD"
                     /locus_tag="VKQ27_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HLY89761.1"
                     /translation="MSNDSHYDERHDDAAPGDEHGSTLQGIQTYVFGLILATGLTATS
                     FWVASTHVLWGPGVPIGLCVLAIAQMGIHLVFFLHITTGPDNTNNVLALAFGVLIVFL
                     VVAGSLWIMANLNSNMLPMDQMDRLTNLQMQR"
     gene            complement(3890..4576)
                     /locus_tag="VKQ27_12335"
     CDS             complement(3890..4576)
                     /locus_tag="VKQ27_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome (ubi)quinol oxidase subunit III"
                     /protein_id="HLY89762.1"
                     /translation="MNASTAPHAPLQPVLDPHRLGHRHGGGGGHGDAAGHGEGGPASK
                     RIIVGYGFWIFLLSDIVMFSAIFASYAVLSGQTAGGPTGAQLFDRTTVAVETGALLIS
                     SFLCGLAMIAANARNMLWTQIGLLVTGLFGFVFIALELREFADMIAHGAGPDRSAFLS
                     AFFTLVGCHGMHVSVGLLWLGTMMAQIWAKGFRPTIMRRLMCFSLFWHALDIIWVALF
                     TIVYLLGTLR"
     gene            complement(4573..6582)
                     /gene="cyoB"
                     /locus_tag="VKQ27_12340"
     CDS             complement(4573..6582)
                     /gene="cyoB"
                     /locus_tag="VKQ27_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HLY89763.1"
                     /translation="MLGKLSWSAIPFDQPIPLMTSIVMILVIGAVLAWITVKGHWPYL
                     WHEWITSVDHKRIGIMYVMLGLVMLLRGFADAIMMRSQLAIAAGPAHGYLPPEHYDQI
                     FSAHGTIMIFFTAMPFVIGLMNFVVPLQLGVRDVAFPTFNSVSLWLTATGVLLTNASL
                     VIGEFARTGWLAYPPLSELKFSPGVGVDYYLWSLQISGIGTLLTGINFVTTILKMRAP
                     GMSYTRMPVFCWTALASSLLIIAAFPVLTATFAMLSLDRYLGFHFFSNDMGGNPMMYI
                     NLIWVWGHPEVYILVLPMFGVFSEVISTFSSKPLFGYRSMILATMAICILSFLVWLHH
                     FFTMGAGANVNAFFGIMTMIIAVPTGAKVFNWLFTMYGGRVRFEVPILWSIGFMITFV
                     IGGMTGVLLAVPPADFVLHNSLFLIAHFHNVIIGGVVFGAFAGYTYWFPKAFGFTLHE
                     GLGKAAFWCWFIGFYLAFMPLYALGLMGATRRMQHYDNMGWQPLMIAAEVGAIIIFCG
                     VLCQIAQLVVSIRTREQRRDLTGDPWDGRSLEWATPSPPPVFNFAVMLDVHGEEAYWS
                     IKQRAIEEQRLAEEPDYKPIEMPRNSPTGFVTAIFATATGFALIWHIWWLVIVGLIGA
                     FATFVWFAWRDEDEYEIPAAEVARIDRERRRVRTEVLLPSLSRPS"
     gene            complement(6585..7553)
                     /gene="cyoA"
                     /locus_tag="VKQ27_12345"
     CDS             complement(6585..7553)
                     /gene="cyoA"
                     /locus_tag="VKQ27_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HLY89764.1"
                     /translation="MRDVPESVIEPVDRSPRGSGRRREAGSGRAFGTLIPTAALLLLA
                     GCSEGVLDPQGPIGSQEKTILLNSLGIMLAIVIPTVLATLGVAWWFRSSNTRAHYRPE
                     WDYSGRVEMIVWSIPMMVILLLGGITWVGSHDLDPPKPLPAPAGAINVDVVSLDWKWL
                     FLYPDQGIASVNRIVVPAGTPVRFRLTAATVMNSFFVPQLGSQIYTMPNMATRLNLQA
                     DKPGSYPGISAQFSGDGFSGMTFTMDAVSPGQFAKWVKTAQASGSALDAAGYAALEKP
                     SQSVAPYTYKSVMPGLFDAIVHTQAAPPEAPRLARTGAKPAPRTEG"
     gene            complement(7720..8679)
                     /locus_tag="VKQ27_12350"
     CDS             complement(7720..8679)
                     /locus_tag="VKQ27_12350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HLY89765.1"
                     /translation="MRALVFETFGGPEVLHEAEVPRPVPTSRQALVRVEYAGLNFADV
                     YRRQGNYHLAGTPPWILGYEGAGVIAELGGEVEGLAVGDRVAFADSPHANAEFAAVDA
                     ERLIPLPDDIGTDTAAALLLQGLTAQYLVADSHIIRPGEIVVVHAAGGGVGLLLVQLA
                     AQYGATVVGLASSEGKRAAAKAAGAAHVFGYDDWKEAILSDVGPADVVYDSIGATLAD
                     SLGVARTGGTVIFYGMAGGDPAPVDPRLLMDRSLTLTGGDLWNVLASAENRRTRATAL
                     FNAIRAGHLTVQIAATYPLTDGADAHRFLQGRGAIGKVLLHIG"
     gene            complement(8837..8962)
                     /locus_tag="VKQ27_12355"
     CDS             complement(8837..8962)
                     /locus_tag="VKQ27_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89766.1"
                     /translation="MRRLDWQTGRPDNLARVGLKALGQPLQPAAQEQIALFDEDI"
     gene            8981..9808
                     /locus_tag="VKQ27_12360"
     CDS             8981..9808
                     /locus_tag="VKQ27_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HLY89767.1"
                     /translation="MDRFGPLSVGEAVEALGVSQPAVTRHAGALVELGLLDASRGDGD
                     QRQRTLALTEAGERLMVQAKRLIWPRIDAAVRDMCNGLSGPFLEQITMLETELGRASL
                     EQRVNRIAAAPAEMPGLSIREYSDELADDFYRINAEWIEQMFVLEENDRQILTRPREL
                     IIDRGGVILFVESAELGVIGTCALIKIEEGRFELTKMGVSESARGLKAGEFLLRAILE
                     RAQNMQIETLYLLTNWKCASAIHLYEKLGFDHDAEIMRIYGSRYERCNVAMAFRGAA"
     gene            complement(9816..10697)
                     /locus_tag="VKQ27_12365"
     CDS             complement(9816..10697)
                     /locus_tag="VKQ27_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY89768.1"
                     /translation="MTDMIEALRTFVRVGERLNFSAVATELNASHTTIARRIDFLETR
                     FAARLFHRSTRALALTDEGLRLLDHGRAILEDIEQAELDLSQGEAGVSGVVRVGVTTA
                     LGLHYAERLATLHARHPSLRIEFAVADWRSNMAEEGLDLALRVGEVPPGMEDVALLGP
                     VDRVLVAAPAYLEERGAPDRPEDLHRHECIGYGYGPSPAMWDVGGLRLRVSGCFRANS
                     SEAVYRAVLSGLGIGLLPHIQVRHDLAAGRLRPLLDGVIIDPLILSVIHRFGRTGLPP
                     RIRVVRDFLIEHFPQPH"
     gene            10822..12333
                     /locus_tag="VKQ27_12370"
     CDS             10822..12333
                     /locus_tag="VKQ27_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HLY89769.1"
                     /translation="MTDRIAHAGLRAKLMSAADAAAKIENGMTVGMSGFTGSGYPKAV
                     PLALADRIEAEHAQGRPMKVRVWTGASTGPELDGALAKADGIELRLPYNSDPIARDRI
                     NRGTMDYLDMHLSQVAPMAWQGFLGPLDLAVIEVTGIRPDGSLIPSSSVGNNKTWIDR
                     AKGVILEVNRWQSEALEGMHDIYYGTALPPHRVPIPLVRPDDRIGESYLRCDPDKVIA
                     VVETDSPDRNAPFSPPDATANAIAGHILEFLRHEVSVGRLPPSLLPIQSGVGNIANAV
                     LSGLLDSPFEDLTAYTEVIQDGMLDLLSAGKLRMASATAFSLSPDAAASLNADMSRFR
                     DKMILRPQEISNHPELIRRLGCIAMNGLIEADIYGNVNSTNVMGSRIQNGIGGSGDFA
                     RNAYVSIFMTPSTAKAGAISAIVPQAAHVDHIMQDVQVIVTEQGLADLRGLSPKQRAV
                     LIIEKCSHPDYRPALRDYYERALRGSYGLHAPSLLSEALSWHQRYVETGSMRL"
     gene            complement(12343..13140)
                     /locus_tag="VKQ27_12375"
     CDS             complement(12343..13140)
                     /locus_tag="VKQ27_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLY89770.1"
                     /translation="MPAIHPVTDPDDPRIEAYRNIRERDLVGRDKLFVAEGKVVLAML
                     LASATYRPSSLLIAEHRLGSLSPLLAQAPDDVPVYTAAQPVLDGIAGFPIHRGILALG
                     RRVHAPSADALLGSLPARADILLLSGIANHDNMGGIFRNAAAFGASAILLDATCCDPL
                     YRKAIRVSVGAALLVPFASIGEDEDPIALLERHQFAPIALTPAGATTLSTYRPQARNA
                     VLLGAEGPGLPPALIARASSVRIPMAPGFDSLNVATTSGIVLHHLLG"
     gene            complement(13264..13500)
                     /locus_tag="VKQ27_12380"
     CDS             complement(13264..13500)
                     /locus_tag="VKQ27_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89771.1"
                     /translation="MSGLNWDGSKDLQRHKKIESLVRGLQETFGSRAEEIAREQFGAA
                     TAGSEAAKAWHLILDELAALPRPGQPPAAEPQQP"
     gene            complement(13570..13809)
                     /locus_tag="VKQ27_12385"
     CDS             complement(13570..13809)
                     /locus_tag="VKQ27_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89772.1"
                     /translation="MFQLLGAISYPLYTIHGPLVSLVQGVNKQLHLGGWGLTLDIMSL
                     GAMALAALPLARWDIRVRAMLTAGFRRGIILGRID"
     gene            complement(13810..14211)
                     /locus_tag="VKQ27_12390"
     CDS             complement(13810..14211)
                     /locus_tag="VKQ27_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89773.1"
                     /translation="MVVVAGDRDQHSICCVSPENVERATRLYLRVLRGGRPMGILKLG
                     TINFGWTWDTLPLGIVRVRYAFPFGVLLFRYRNHIPQYIGRVLPVAPLTLLAVVLFGQ
                     EHLALDIALLVVGARLLIAVGFRSTAGRRYG"
     gene            14328..14501
                     /locus_tag="VKQ27_12395"
     CDS             14328..14501
                     /locus_tag="VKQ27_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89774.1"
                     /translation="MKDQYLLHAQRDDQSHAKERIEADQPFLAEGEAAQIAIKPCVIG
                     HRHDEAAEHEEQV"
     gene            complement(14498..15424)
                     /locus_tag="VKQ27_12400"
     CDS             complement(14498..15424)
                     /locus_tag="VKQ27_12400"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY89775.1"
                     /translation="MSVNMGRWTLKRIEDAFTEFFRRVKRDQKAGFPPYRSRYRWRSF
                     GLLEASGLRIKGHYLLLRGITRALRMNLHRPLPLDATLKGATFTRRGRHWFVSLQIES
                     QTIIAATAPAGRAIGLDVGVEHLASWSDGTIDGHVVNARPRSRRERDLRLAQRALARC
                     RRGSRRRAKVRERLARVQASVARQRDTHLHQQAERLARSHRLIVIEKLSIRNMTRSAA
                     GTVDAPGTNVAAKAGLNRSILDASMGRFLQYLRYKAARAGGTVLEVRAAGTSQQCSAC
                     GTKVPKTLSIRDHRCGCGLEIQRDVNAARRRT"
     gene            complement(15681..15815)
                     /locus_tag="VKQ27_12405"
     CDS             complement(15681..15815)
                     /locus_tag="VKQ27_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89776.1"
                     /translation="MSDQDHWPLLDAIRGVAALAVVWFHLHFVVGYDCAGAPGGRLGF
                     "
     gene            complement(15959..17290)
                     /locus_tag="VKQ27_12410"
     CDS             complement(15959..17290)
                     /locus_tag="VKQ27_12410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY89777.1"
                     /translation="MSTPPRLRRDRWRVGTIGIWGGKMEYGDHHCRHCGTALEICVAD
                     LGLMPIANDLIDRSLPSQEAPKAPLVAMVCGECRLVQTIDYKAANDIFRDDYVYFSSE
                     SISWLEHARKYVGEMAQRFGLDGDSRHIEIASNDGYLLQYSQSLGIPTLGVEPCASVA
                     TVARDKGIDTRQAFFSQALAGQLVAEGWHADLVTANNVFAHVPDVNDFAAGIRTLLMP
                     NGVATVEVQHLLRLMQRNQFDTIYHEHFSYYSLLSAKRVFEAAGLRVFDVDELPTHGG
                     SVRFYLCRDDSSVQTLPSVARVLAEEIAYGLDRDEVYVGFSKQISQLKQDLVELLDRL
                     KQDGKKILGYGAPAKGVILLNYCGISTGIIDFTVDKAPSKQNKILPGVGIPILHPKML
                     ESEEPDYILVLAWNIADEIIAQIRAAFPYQGAFIIPMPKPHIIQGVVSVAA"
     gene            17736..18434
                     /locus_tag="VKQ27_12415"
     CDS             17736..18434
                     /locus_tag="VKQ27_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY89778.1"
                     /translation="MPSQGAITILGRLPIAIEGLKRILTEYGFDVIGSGMDIASIPKS
                     IVQEGDFHIVIVDELIDVHGPKYVGEVLKTFRDARIVIASNAFDFATMAACFREGAFG
                     YIVKSISSKSLVASLQLVAAGEKVMPTSLVDALPDHPGGGYDSGYTSPSFRDVGLSQR
                     EAEILTYLVNGQPNKKISRDLKISEATVKVHVKAILRKVGVQNRTQAAIWAMGEKEDS
                     ANTGEGEPSHNGHP"
BASE COUNT         3575 a         5750 c         6167 g         3250 t
ORIGIN      
        1 gggcgctgca acttcaccaa cgtggaggcg gtgccggatc gctatctttc ggccaatccg
       61 cacttcgcca agtcggcgct gggccgctat cggccggaac agttcatcga gctggtcgat
      121 cgctacggca tcgcctggca cgagaagacg ctgggccagc tgttctgcga cggttcggcg
      181 cgacagatcg tggcgatgct gctcgacgag tgtgccgtgg gcgatacgac catccattgc
      241 ggcgatccga tccgcgacat tgcgcatgct gacggtcgct tcacggtgac cttcgggggg
      301 caggccgcaa cagcgccggc gctggtgatt gccactggcg gcccgtctat tcccaagatg
      361 ggcgcgacgg gcttcgctta tgatgtcgcg aggcgcttcg ggctcaaggt ggtcgagccg
      421 cggccggcgc tggtgccgct gacgctgggc ggcgatgagg cgctgttccg ctcgctctcg
      481 ggcgtggcga gcgacgtggt cgcacgggcc ggcaagacga gcttccggga ggcagcgctg
      541 ttcacccatc gcggcctgtc ggggccggcg attctgcaga tctcgtccta ctggcgacat
      601 ggcgagaacc ttggcatcga tttcctgccc tcggccgagg cgggctggct gcgcgaggcg
      661 aagcgcgcgc ggccgcgtgc ggggttacgc agcgtgcttg gtgggctgtt gccggatcga
      721 ctggcggaaa cactggcgga gcgcctcgac ctcgttggtg agcttgccaa cctttccgac
      781 cgggcgctgg aggcggccga gcgccggctg gcggactggc gcttccagcc caacggcacc
      841 gaaggcttcg ccaaggccga ggtgacggtc ggcgggatcg ccactactgg cctgtcgtcg
      901 cagacgatgg aggcgcggac ggtgcccggg ctctacgcga tcggcgaggc ggtggatgtc
      961 accggctggc tcggcggcta caattttcag tgggcctggg cgagcggctg ggcagccgga
     1021 caggcgctct agctcttctc gggcttcttt gcggcaggtg ccggcggtgc gccgatgaat
     1081 tcgaaacgct gcggcgcagt gccgagctcg atccccacgg cgggcagttt ctcaaggagc
     1141 tcgaacagga ggtggctgcg cgtcgcatag acctcgcgcg ggctgtggac atagccgaag
     1201 ctcatcaggt tgatccgccc gtcgatgatc gaatcgatga agaggctggg ggccgggtcg
     1261 ttgagcacag cgtgcgtctc ggcgaacatc cgcagcacca tctctcgcac ctgaccgata
     1321 tcggtgccca tcggcaccga gaagttgagt tgcacgcgcc caatcggatc ggccagcgtc
     1381 atgttgcgga tcgtcttggt gatcagctcc gaattgggga cgatcagggt cgatcgatcg
     1441 gcgatctgga tctcggtggc gcgcacgctg atccgcttca cgtcgccctc ctgatcgccg
     1501 atgcggacga ggtcgccgat cttcaccggg cgctcggcga gcaggatcag gcccgagacg
     1561 aaattctgcg tgatcgcttg caagccgaag ccgatgccga ccgacagggc tgacagcagc
     1621 agcgcgatcc gttccatccc gatgccgagc gaggccagcg accacagcac cgccaggatc
     1681 aggccggtat agcgcgccac catgctgatc gagttgcgcg cgccggcatc gagctcggtc
     1741 gcgggcagat agcgattggt cagccaatgc tgggccgagc ggaccacgaa cagcccgacg
     1801 aacagcaccg cgccggcccg gaagatcgca cccggcgcga tggtgatctg cccgatggtc
     1861 acgccctggg cgatgcggtc gctctgggcg agaagcgtgg tgaggtttga accaaacggc
     1921 gcgaagatca ggctcgcagc gatcgcgatc aggaccagcc gcagcagcgc cgagagcgcg
     1981 acgccgaact ggtcgatcag gctacgacgc agaccgaagc cgtgatggag gttgtccgcc
     2041 agccggctgt cgcgccggaa caggttgctg cagatatcgt cgacagcgag cagcgagagg
     2101 tagagcgtcg aggccaccac cgcgacccac acgacgaagc gcgacaggaa ataggcgagg
     2161 ctgacatagc ctgccgacat cgccaccacc gcgatgatga cgacggccca tgccgccagc
     2221 gagacgaagg cgaatccggc ctggctggtc tcatgttctt cgtctgcgcg ggtgcgatgc
     2281 gagcgcaacc ggccaatcgt gagcagtagg ccgagcacca gcgcgatata gacaagggcg
     2341 gcggcactgt cggctgcggc acgcgagggc ccgcttgcgc cgatcgcgtc gcggaatgcg
     2401 atcagcagca cgcttgtcag cacggcaccg gccgcggcca ccgcccatgg cagcagcgct
     2461 ttggccgcat ggtcagacag ccccaccagc cgccatgagc cctgcttggg ctggagcagc
     2521 gcgcccgcca ccgctgtgat gagggcggcg acgaacactg ctcgttggaa gctctgcgcg
     2581 aggccctccc atccgggcgc cagcatgccg ccgccacgca atccctcgat cagcgcgacg
     2641 gtggctaggc cgggaatgca ggcgccgaca gtggcccgcc acaaggcgag gccggaccgg
     2701 cggacgcggc tccccggcac atgctcgatg acatagcggc gcccggtcgc ccgcagcgcc
     2761 atccggatcg gtccagccag cgcgatcgcg atggcgaggc cgatcagcgc ggggatcagc
     2821 ccgtcgcccg acaggccgcg acggagcgct tcgccttcgg cagccagcaa tgccgcgacc
     2881 cgccgcccgt cgcgcggaga ggcgtgcagc agcgccgtcc agaataccgg ggtcagaggc
     2941 gacgccacgc gttccgtcat ccgttcgcta aaggcgttgg cctggctttc gccgatctcg
     3001 accgcgagct gatcggcctc gacgcccagc agcttgccac gcttgatcgc cgaatcgacg
     3061 gtcgagcgct gctgggcgag cagcttgcgc tgggcacgga tgtcgggcgc ctcgacgacg
     3121 ccgggagtga cgggccccaa ctccgcgatc cgggcgtcaa tcagtcctag ctgagttgtg
     3181 agcgtggcaa cctggtcggt ggttgtctgc ttcaccgtgt tggcgcggtc ctgaaggctt
     3241 tgccgttcgg tagtgctgag gttgccgtta aaggctccgt cgatcgcctg gaactcgtcg
     3301 gccgcctggt cgagtgactg ggtcgccgtg gcgatcgggt ctgcatcctg cgcgatcgcc
     3361 ggggtggcga ggaggagcga gagagcgagg agaaggcgcg cgatcatgcg cgtcttccta
     3421 tcgcctccga aacgctccgc ccagcccact tggcgggttt gccgctcgcc tagtcgcgag
     3481 agcgcgcttc atcgctgcat ctggaggttc gttagccgat ccatctggtc catgggcagc
     3541 atgttggaat tgaggtttgc catgatccac agcgaacccg cgaccaccag gaagacgatc
     3601 agcacgccga acgcgagagc cagcacgttg ttggtgttgt ccggaccggt ggtgatgtgc
     3661 aggaagaaga cgaggtgaat gcccatctgc gcgatcgcca gcacgcacaa gccgatcggc
     3721 accccgggcc cccacagcac atgggtggac gctacccaga acgacgtcgc ggtcagcccc
     3781 gtcgccagga tgaggccgaa gacataggtc tggatgccct gaagtgtcga tccatgctcg
     3841 tcgccggggg cggcgtcgtc atgacgctcg tcataatggc tgtcgttgct catcgcaggg
     3901 ttcccagcag atagacgatg gtgaacagcg cgacccagat gatgtcgagc gcatgccaga
     3961 acaggctgaa gcacatcagc cggcgcatga tggttgggcg gaagcccttg gcccagatct
     4021 gcgccatcat cgtgccgagc cacagtaggc cgacgctgac gtgcatgccg tggcagccga
     4081 ccagcgtgaa gaaggccgag aggaaggcac tgcggtccgg cccggcgccg tgcgcgatca
     4141 tgtcggcgaa ctcgcgtagc tcgagcgcga tgaagacgaa gccgaacagt ccggtgacga
     4201 gcaggccgat ctgcgtccac agcatgttgc gagcgttcgc cgcgatcatc gccagcccac
     4261 acaggaagct tgagatgagc agcgcaccgg tctcgaccgc gacggttgtg cggtcgaaca
     4321 gctgcgcgcc ggttggtccg cctgcggtct ggccggagag taccgcgtag gacgcgaaga
     4381 tcgccgagaa catcacgatg tcggacagca ggaagatcca gaagccgtag ccgacgatga
     4441 tccgcttcga cgcggggccg ccttcgccgt ggccggcggc atccccgtgg ccgcctccgc
     4501 cgccgtgacg gtggccgagc cggtgcggat caaggacggg ttggagcggg gcgtggggtg
     4561 cggtgctcgc gttcatgagg ggcggctcag gctggggagg agcacttcgg tacgcacgcg
     4621 ccgccgttcg cggtcgatcc gtgccacctc tgcggcgggg atttcatatt cgtcctcgtc
     4681 tcgccaggcg aaccagacga aggtcgcgaa cgccccgatc aggccgacga tcaccagcca
     4741 ccagatgtgc cagatcagcg cgaagccagt cgcggtggcg aagatggcgg tgacgaagcc
     4801 ggtcgggctg ttgcgcggca tttcgatcgg cttgtaatcg ggctcctcgg cgaggcgctg
     4861 ctcctcgatc gcgcgctgct tgatgctcca ataggcctcc tcgccatgga catcgagcat
     4921 caccgcgaag ttgaacaccg gcggcggcga gggggttgcc cattcgagcg agcggccgtc
     4981 ccagggatcg ccggtcagat cgcgacgttg ctcgcgcgtg cggatcgaaa cgacgagctg
     5041 cgcgatctgg cagaggacac cgcagaagat gatgatcgcg cccacttcgg cggcgatcat
     5101 aagcggctgc cagcccatat tgtcataatg ctgcatgcgg cgcgtcgcgc ccatcagacc
     5161 cagcgcgtag agcggcatga aggccagata gaagccgatg aaccagcacc agaaggccgc
     5221 cttgccaagg ccttcgtgca gcgtgaagcc gaacgccttg gggaaccagt aggtgtagcc
     5281 cgcgaacgcg ccgaacacga cgccgccgat gatcacattg tggaagtgcg cgatcagaaa
     5341 cagcgagttg tggagcacga agtcggccgg aggcaccgcc agcaatacgc cggtcatgcc
     5401 gccgatcacg aaggtgatca tgaagccaat cgaccacagg atcggcacct cgaagcggac
     5461 acgcccgccg tacatcgtga acagccagtt gaacaccttg gcgccggtcg gcactgcgat
     5521 gatcatcgtc atgatgccga agaatgcgtt gacgttcgcg cccgcgccca tcgtgaagaa
     5581 atggtgcagc cacaccagga acgacaggat acagatcgcc atcgtggcga ggatcatcga
     5641 gcggtagccg aacaagggtt tgctcgagaa ggtggagatc acctcggaga acaccccgaa
     5701 catgggtagg acgaggatgt acacctccgg atgtccccac acccagatga ggttgatgta
     5761 catcatggga ttgccgccca tgtcgttgga aaagaagtgg aaacccagat agcggtcgag
     5821 cgacagcatc gcgaaggtcg cggtcagcac cgggaaggcg gcgatgatca gcaggctgga
     5881 ggccagcgcc gtccagcaga acaccggcat gcgcgtgtaa ctcatgcccg gcgcgcgcat
     5941 cttgaggatg gtcgtgacga agttgattcc cgtcagcaat gttccgatgc ccgatatctg
     6001 gagcgaccag agatagtagt cgacacccac gccgggagag aatttgagct cggacagcgg
     6061 cggataggcg agccagccgg tgcgcgcgaa ctcgccgatc accagcgagg cgttggtcag
     6121 cagcacgccg gtcgcggtca gccacaggct caccgagttg aaggtcggga acgcaacgtc
     6181 ccgcacaccc aattgaagag gcacgacaaa attcatcaag ccgatgacga agggcatcgc
     6241 cgtgaagaag atcatgatcg tgccgtgggc cgagaagatc tgatcataat gctcgggtgg
     6301 cagatagccg tgagcaggcc cggcggcgat cgcgagctgg gaacgcatca tgatcgcgtc
     6361 ggcaaaaccg cgcagcaaca tgacgagccc gagcatgacg tacatgatgc cgatgcgctt
     6421 gtgatcgacc gaggtgatcc actcgtgcca cagatagggc cagtggccct tcacggtgat
     6481 ccaggcgagc accgcgccga tcaccaggat catcacgatc gaggtcatca gcgggatcgg
     6541 ctgatcaaac gggatcgcgg accagctgag ctttccgagc atgttcagcc ctccgtccgg
     6601 ggagcgggtt tggcgccggt ccgggcgagg cggggcgctt cgggtggtgc ggcctgggtg
     6661 tggacgatgg cgtcgaacag tcctggcatc accgacttgt aggtataggg cgcgaccgac
     6721 tggctgggtt tctcaagcgc cgcatagccg gcagcgtcga gcgccgaccc gctggcctgc
     6781 gccgtcttca cccatttggc gaactggccc ggagacaccg catccatcgt gaaggtcatg
     6841 cccgaaaagc catcgccgct gaattgtgcg gagatgccgg gatagctgcc gggcttgtcc
     6901 gcctgcaggt tcagtcgtgt ggccatgttg ggcatggtgt agatttggct gccgagctgc
     6961 ggcacgaaga agctgttcat taccgtcgcc gcggtcaacc ggaaacgaac cggcgtgccg
     7021 gcgggcacga cgatgcgatt gacgctggcg atgccctgat cgggatagag gaacagccat
     7081 ttccagtcga gcgagacgac gtcgacgttg atggccccgg ccggcgccgg gagcggcttg
     7141 ggtggatcaa ggtcgtggct gccgacccag gtgatgccgc ccagcagcag gatcaccatc
     7201 atcgggatcg accacacgat catctcgacc cgccccgaat agtcccattc ggggcggtag
     7261 tgggcgcggg tgttggagga gcggaaccac caggccactc ccagcgtcgc cagcacggtg
     7321 ggaatgacga tcgccagcat gatgccgagc gagttgagga ggatggtctt ttcctgcgat
     7381 ccgatcggtc cctggggatc gagaacccct tccgagcagc cggcgagcag gagaagtgca
     7441 gcggtcggga tcagtgtgcc gaatgcacgg ccggatcctg cttcacgcct tcggccgctg
     7501 ccgcgtggcg agcggtcgac gggctcgatg acactctccg gcacgtcgcg catggagtaa
     7561 tatcccctga agcgtcggct gatcgtttcc gtcgctccat agcctattag gcgacgacga
     7621 gcacctgttc ttgtatgttt gtgacgtgcc agcttgttac gtgccccttt ccacggctga
     7681 tcaagctgat gagaggcact gccgggctgc cgggctgcat cagccgatgt ggagcagcac
     7741 cttgccaatc gcgccacggc cctggaggaa gcgatgggcg tccgcgccgt cggtaagcgg
     7801 ataggtggcg gcgatctgca cagtgaggtg gccagcgcga atggcgttga acagcgcggt
     7861 ggcgcgcgtc cggcgattct cggcggaagc gagcacgttc cagaggtcgc cgccggtgag
     7921 cgtgagtgat cgatccatca gcaggcgggg atcgaccggc gcaggatcgc cgcctgccat
     7981 gccgtagaag atcaccgtgc cgcccgtcct cgcgacgccg aggctgtccg cgagtgtcgc
     8041 tccgatcgag tcgtacacga catcggccgg accgacgtcg ctgagaatgg cctccttcca
     8101 gtcgtcatag ccgaaaacat gtgcggcacc ggcggccttc gcggccgccc gcttcccctc
     8161 gctcgatgcg aggccgacga cggtcgcgcc gtattgggcg gctagctgga cgaggagcag
     8221 cccgacaccg ccgccggcgg cgtgcacgac cacgatctct cccggccgta tgatatggct
     8281 gtcggcgacg agatattgcg ccgtgagccc ctggagcagt agcgcggcgg cggtgtccgt
     8341 tccgatgtcg tcgggcagcg ggatcagtcg ctcggcatcg acggcggcga attcggcatt
     8401 ggcgtgaggg ctgtccgcaa aggccacgcg gtcgccgacc gctagccctt cgacctcgcc
     8461 gccgagttcc gcgatcacgc ccgcgccctc atagcccagg atccacggcg gcgtgccggc
     8521 gagatgatag ttgccctggc ggcgatagac gtccgcgaaa ttgagcccgg catattcgac
     8581 gcgcacgagc gcctggcgcg aggtcggcac aggacggggc acttcagcct cgtggagcac
     8641 ctcgggccct ccgaacgtct cgaagaccag cgcgcgcatc cgttcactcc tgtccgatgg
     8701 aaagcgtgcc ccttatcgcg ctcggcgtct ctcagccaaa tttttgcgac gacgtgcacg
     8761 ccgtcgggct attcccgttt tctataacgc gatatatagc atgttatatt tatcggagag
     8821 gccgcgaaga atggtttcag atatcctcgt cgaacagggc aatctgttcc tgggcagccg
     8881 gctgaagcgg ctggccgagc gccttcaggc cgacgcgggc aaggttatcc ggtctgccgg
     8941 tctgccaatc cagccggcgc attttccgct gctcgcagcg atcgatcgct tcggaccgct
     9001 cagcgtcggc gaggcggtcg aggcactggg tgtgagccag ccggcggtga ctcgccatgc
     9061 gggcgcgctt gtcgaactcg gcctgctcga cgcgagccgc ggcgatggcg accagcgcca
     9121 gcggaccctc gcgctgacgg aggcgggcga gcgcctgatg gtgcaggcca agcggttgat
     9181 ctggccgcgg atcgacgctg cggtgcggga tatgtgtaac ggcctttcgg gcccctttct
     9241 ggagcagatc accatgttgg aaacggaact cggccgcgcc tcgttggagc agcgtgtgaa
     9301 ccgtatcgct gccgcgccag ccgaaatgcc cggtctgtcg atccgcgaat attcggacga
     9361 gctggccgac gatttctacc ggatcaacgc cgagtggatc gagcagatgt tcgtgctcga
     9421 ggagaatgac cggcagatcc tgacgcgccc gcgcgagctc atcatcgatc ggggcggcgt
     9481 gatcctgttc gtggagtcgg ccgagctcgg cgtgatcggc acctgcgcac tgatcaagat
     9541 cgaggagggc cgtttcgagc tcaccaagat gggcgtgtcg gaaagcgcgc gcggtctcaa
     9601 ggcgggcgag ttcctgctcc gcgcgatcct tgagcgggcg cagaacatgc aaatcgagac
     9661 gctctacctg ctcaccaact ggaagtgcgc ctcggcgatt catctctatg aaaagctcgg
     9721 cttcgatcat gatgccgaga tcatgcggat ctatggaagc cgctacgagc gctgcaacgt
     9781 ggcgatggcg tttcgcggcg cagcgtgaac ggccgtcagt ggggctgcgg gaaatgttcg
     9841 atcaggaaat cgcggacgac gcggatccgg ggcggtagtc cggtcctgcc gaagcggtgg
     9901 atgacggaca ggatcagcgg atcgatgatg acgccgtcca gcaatggcct gagccggcca
     9961 gcggcgagat catgccgcac ctggatatgc gggagcaggc cgatgccaag tccgctcaac
    10021 acggcgcgat agaccgcttc gctgctgttg gcgcggaagc atccggagac gcgcagccgc
    10081 aggccgccca cgtcccacat cgccgggctc gggccatagc cataaccgat gcactcgtgg
    10141 cgatgcaggt cttcgggtcg atcgggtgcg ccgcgctcct ccagataggc gggcgcggca
    10201 accagcacgc gatcaaccgg gccgagcaga gcgacgtctt ccattccagg cggcacttca
    10261 ccgacgcgga gcgcaagatc gagtccttcc tcggccatgt tgctgcgcca gtcggcgact
    10321 gcgaattcga ttctcagact cgggtggcga gcatgaagcg tcgccagacg ctccgcataa
    10381 tgcaggccga gcgccgtcgt tacgccgacc cggaccaccc cgctcacgcc ggcttcgccc
    10441 tggctcagat ccagctcggc ctgctcgata tcctccagga tcgcgcggcc atgatccagc
    10501 aagcggagcc cttcgtcggt gagcgccagc gcccgggtgg atcggtggaa cagccgcgct
    10561 gcgaagcgcg tctccagaaa atcgatgcgt cgtgctatcg tcgtgtggct cgcattgagt
    10621 tcggtcgcga ccgcggagaa gttcaggcgt tctcccacgc gcacaaaggt tcgaagcgcc
    10681 tcgatcatat ccgtcatctg aacgtcctgt gcacaactga cctgcgtatt atcgtccttg
    10741 tttctatccg cgcacaagcc gagcaagcgt tcaaaggcag cggtaaggca cctgccgccg
    10801 atacgatagg agacgcattc gatgactgat cgcatcgccc atgctggcct gcgcgcgaag
    10861 ctgatgagcg cggcggacgc cgccgcgaag atcgagaacg gcatgaccgt cggcatgagc
    10921 ggcttcactg gttcgggcta tcccaaggcg gtgccgctgg cactggcgga tcgcatcgag
    10981 gcggagcacg cccaagggcg gccgatgaag gtgcgggtgt ggaccggcgc gtcgaccggg
    11041 cccgaactcg acggcgcgct cgccaaggcg gatggcatcg agctcaggtt gccctacaat
    11101 tccgatccga tcgcgcgtga tcgcatcaat cgcggcacga tggactatct cgacatgcac
    11161 ctgagccaag tggcgccgat ggcgtggcag ggctttctgg ggccgctcga tctggcggtg
    11221 atcgaggtga cgggcatccg gcctgacgga tcgttgatcc cgtcctcgtc cgttggcaac
    11281 aacaagacct ggatcgatcg cgccaagggc gtgatcctcg aggtcaatcg ctggcagagc
    11341 gaggcgctgg aggggatgca cgacatctat tacggcaccg cgctgccgcc gcaccgcgtg
    11401 ccgatcccgc tggtccgccc cgacgaccgc atcggcgaga gctatttgcg ctgcgatccc
    11461 gacaaggtga tcgcggtggt cgaaaccgac tcgcccgatc gcaacgcccc gttcagcccg
    11521 cccgatgcga cggcgaacgc gatcgccggg cacattttgg aattcctgcg ccacgaggtg
    11581 tcggtgggcc gcctgccgcc gtcgctgctg ccgatccagt caggcgtggg caacatcgcc
    11641 aatgcagtgc tgagcggcct gctcgacagc ccgttcgagg atctgaccgc ctataccgag
    11701 gtgatccagg acggcatgct ggacctgctt tcggcgggca agctcaggat ggcgtcggcg
    11761 accgccttct cgctcagccc cgacgcggcg gcttcgctca acgccgacat gtcgcgcttc
    11821 cgcgacaaga tgatcctgcg cccgcaggag atcagcaacc atcccgaact gatccggcgg
    11881 ctgggctgca tcgcgatgaa cgggctgatc gaggccgaca tctacggcaa cgtcaattcc
    11941 accaacgtca tgggatcgcg catccagaac gggatcggcg gatcgggcga cttcgcgcgc
    12001 aacgcctatg tctcgatctt catgacgccg tcgaccgcga aggcgggagc gatctcggcg
    12061 atcgtgccgc aggccgcgca tgtcgatcac atcatgcagg acgtgcaggt gatcgtcacc
    12121 gagcaggggc tggccgatct gcgcgggctg tcgcccaagc agcgggcagt gctgatcatc
    12181 gagaaatgct cgcacccgga ttaccgcccg gcgctgcgcg actattatga gcgcgcgctg
    12241 cgggggtcat acgggttgca cgcgccctcg ctgctgtccg aggcgctgtc ctggcaccag
    12301 cgctacgtcg agacgggatc gatgcgcctc tgaacggcgc cgtcagccga gcaggtggtg
    12361 gagcacgatc ccgctcgtcg tcgcgacgtt gagggaatcg aagcctggcg ccatcgggat
    12421 gcggacgctg ctggcgcggg cgatcagcgc gggtggcagg cccgggcctt cggcgcccag
    12481 cagcactgcg ttgcgcgcct gggggcgata ggtggagagc gtcgtcgcgc cggccggggt
    12541 cagcgcgatc ggcgcgaact ggtgccgttc gagaagcgcg atcggatcct cgtcctcgcc
    12601 gatggaggcg aacggcacga gcagcgcggc gcccaccgaa acgcgtatcg ccttgcggta
    12661 cagcggatcg cagcatgtcg cgtcgagcag gatcgcgctc gcgccgaagg ccgcggcgtt
    12721 gcggaagatg ccacccatat tgtcatggtt ggcgatgccc gacagcagga ggatgtcggc
    12781 ccgcgccgga aggctgccga gcagcgcgtc ggcgctcggc gcatgaacgc gccggcccag
    12841 cgccaggata ccacggtgga tcgggaagcc ggcgatcccg tccagcaccg gctgcgcggc
    12901 ggtatagacc ggcacatcgt cgggagcctg cgcgagcagg ggggataggc ttcccagccg
    12961 atgctcggcg atcagcagcg atgacggccg gtaggtcgcc gatgcgagca gcatcgcgag
    13021 cacgaccttg ccttcggcca cgaacagctt gtcgcggccg accagatcgc gttcgcggat
    13081 gttgcgatag gcctcgatcc ggggatcatc tggatcggtg acgggatgga tggcaggcat
    13141 tgcggctttc gaagctgtga caggcgtgac gtgcgggctc gaacgtctcg cgcgccgcga
    13201 cggcctagca gaccggctct tggatgaaag gcgtgattcc cgcgctctgg atcgggcggg
    13261 aagtcagggc tgctgtggtt cggccgcagg cggctggccg gggcggggaa gggccgccag
    13321 ttcgtcgaga atcaggtgcc aggccttggc cgcctcgctg cctgcggtcg ccgcgccgaa
    13381 ttgctcgcgg gcgatctctt ccgcgcgcga tccgaaggtt tcctgaagcc cccgcacgag
    13441 gctttcgatc ttcttatgtc gctggaggtc tttcgaaccg tcccagttca atcccgacac
    13501 tatgcccatc ctgctgactg gtgtctgatc gaccttctat cagtgtcggg cgaatcggtg
    13561 ccctacgatt tagtctatac gtcctaaaat gatccctcgc cggaacccgg ccgtcagcat
    13621 ggcacggacc cgtatgtccc accttgcaag cggcagcgcc gccagcgcca tcgcaccgag
    13681 cgacatgata tcgagcgtca agccccatcc tcccaaatgc agctgcttat tcacgccctg
    13741 caccagcgac acgagcggac catggatggt gtatagcgga taggaaatgg ccccgagcag
    13801 ttggaacacc tagccatagc ggcggcctgc cgtgctccga aacccaaccg caatcagcag
    13861 gcgcgcgccg acaaccagca atgcgatgtc gagcgccaga tgctcctggc cgaacaagac
    13921 cacggccaat agcgtgagcg gggcaaccgg taagacacgc ccgatatatt gtggaatatg
    13981 gttgcgatac cggaacagga ggacgccgaa gggaaaggca tatctaaccc ggacgattcc
    14041 cagcggcaag gtatcccatg tccagccaaa gttgatcgtt cctagtttta aaatgcccat
    14101 aggacggccg ccccgcaaga cgcggagata aaggcgagta gcccgttcaa cattctccgg
    14161 tgaaacgcag caaatagaat gttgatcgcg atctccagcg acaacgacca tgctggcccg
    14221 tcaatgggga aagtccaacc tgttccgctg ttgaacggcg acggaagcca gaatgcctca
    14281 aacatggaag acagcactgt gtcctgctct gtcaggctgc ctgctcggtg aaggatcagt
    14341 atctgctgca cgcccaacgc gatgatcaat cccacgcgaa agagcggata gaggcggatc
    14401 agccgtttct agcagaaggc gaggcagctc agatcgcgat caagccttgc gtgataggcc
    14461 atcgccacga cgaagccgct gagcatgaag aacaagtcta ggttctccgc cgcgctgcat
    14521 tcacatcccg ttggatctcg agaccacatc cgcagcgatg atcgcggatg ctgagcgtct
    14581 tcgggacctt tgtcccgcag gcgctgcact gctggctggt gccagcggct cgaacctcca
    14641 gcaccgtgcc accggctctc gcagctttgt agcgcagata ctggagaaaa cgtcccatcg
    14701 aggcgtcgag gatcgagcgg ttgagcccgg ccttggccgc cacgttcgtg cccggcgcgt
    14761 caactgtgcc agccgccgaa cgggtcatgt tccggatcga gagcttctcg atgacgatca
    14821 gccgatgcga tcgcgccagg cgctctgcct gctgatgcag atgggtatcc cgctgtcgag
    14881 caacactggc ttgcacgcga gccaggcgtt cacgcacttt ggcccggcgc cggcttccgc
    14941 gccggcagcg tgcgagggcg cgctgggcca ggcggagatc acgctcgcgc cgtgatcgcg
    15001 ggcgcgcgtt gacgacatgc ccatcgatag tgccatcgct ccagctggcc agatgctcaa
    15061 cgccgacatc gagaccgatc gcgcggccgg ctggcgcggt ggcggcgatg atcgtctggc
    15121 tctcgatctg aagcgacacg aaccaatggc ggccgcgcct agtgaaggtg gcgcccttga
    15181 gcgtggcatc cagcggcaac ggccgatgga ggttcatgcg gagcgctcgg gtgataccgc
    15241 gcagcagcag atagtgacct ttgatccgta atccggacgc ctcgagcagg ccaaaactgc
    15301 gccagcgata gcggctgcga tagggcggaa agcccgcctt ctggtcgcgc ttcactcggc
    15361 ggaagaactc cgtgaaggca tcctcgatgc gcttgagcgt ccagcgaccc atgttgacgg
    15421 acagtgcgcc tgaccatcag gatccgccct gcgaatctcg gtcagcgacc tttgctggtc
    15481 gagacgagtg atggatttcc gagccttgga ataagctccg atccgctctt ccaacgccgc
    15541 attatagagt tcacgctgca tctcgctcaa cgccgcgagc tgggcatcct gcccggccga
    15601 cggccgcagt ttgtagcgat atgtcaccag catggttcat gacatgccgt tcctactttg
    15661 ttccatcaac cggatttcct ctagaaccca agtctaccgc caggggcccc cgcacagtca
    15721 tagccgacga cgaaatggag atggaaccag acgacggcaa gcgcagccac gccacgaatg
    15781 gcatcaagca gcggccaatg gtcttgatcg gacatgccac cacccggaga ccttcgattc
    15841 tattcccggt ttgtgctgca aagccggcgg cccgcccggt gcagtgctgg catgcggtgc
    15901 aactgatcgt gaaaatgcct ggcgttcgta accgccggca tatccgatgg agccgcagct
    15961 atgccgctac gctgacgaca ccctgaatga tatgcggctt gggcatcgga atgatgaagg
    16021 ccccctgata ggggaaggca gcccggatct gcgcgatgat ctcgtcggca atgttccatg
    16081 caaggaccag gatatagtcg ggttcctcac tttccagcat tttcgggtgc aggattggaa
    16141 tgcccacgcc gggcagaatc ttgttctgtt tggagggcgc cttgtcgacc gtaaaatcga
    16201 tgatgccggt cgagatcccg caataattga gtaggatcac gcccttggcc ggcgcaccat
    16261 aacccaggat ctttttaccg tcctgcttga ggcggtcgag aagctccaca aggtcctgct
    16321 tcagctgaga aatttgcttc gagaagccga cataaacctc gtcacggtca agcccgtacg
    16381 cgatttcctc ggccagcacc cgcgcgacgc tgggcagcgt ctggaccgag ctatcgtccc
    16441 ggcagaggta gaaacggacg gagccgccat gtgtgggaag ctcgtcgaca tcgaacaccc
    16501 tgaggcccgc cgcctcgaag acacgtttgg cgctcagcag agaataatag gaaaaatgct
    16561 catggtagat cgtgtcgaac tggtttcgct gcatgaggcg gaggagatgc tgcacctcga
    16621 cggtcgccac tccattgggc atgaggagcg tccggatacc tgccgcgaag tcgttgacgt
    16681 cggggacatg cgcgaaaaca ttgttcgcgg ttacgagatc cgcgtgccag ccctcggcga
    16741 cgagctgccc ggctaatgcc tgggagaaaa atgcctggcg ggtgtcgatg cccttgtcgc
    16801 gcgccaccgt cgcaaccgag gcgcatggct cgacgcctag ggtgggaatg ccgagcgatt
    16861 gggaatattg cagcagatag ccgtcgttgc tggcgatttc gatatgccgg gaatcgccat
    16921 ccaacccgaa gcgctgggcc atctccccga catatttgcg ggcatgctcc agccacgaaa
    16981 tcgactccga ggagaagtaa acataatcat cgcggaagat gtcgttggcg gccttgtagt
    17041 cgatggtctg gaccagtcgg cattcaccgc acaccatcgc gaccaggggc gcttttggcg
    17101 cttcctgaga gggtagtgac ctgtcgatca ggtcgttcgc gatcggcatc aatccaagat
    17161 ccgccacgca aatctcaagt gcggtgccgc agtgacggca gtgatgatca ccatactcca
    17221 tcttgccccc ccaaatgcca atggtgccga cacgccagcg gtcgcgccgc agccggggcg
    17281 gcgtactcac aatcgccaag gatggaacca tgcgaagttg ggcgcagcaa tcatctaagt
    17341 ttggctaccg accactattt ggaggtacaa tcagcgcccg cgaagccacc gtatctcccg
    17401 ttgtctcagg ggtttggcag gaggtgtccg taaatcatcc actttagtca tgagtaggat
    17461 ccacgtaggt ggagatgtta tgatagatca attggatatt caatccagat tctacgggtg
    17521 tattttcttc aaatttgtcg atcagaattt tctatattat catttaaccg taccaaccta
    17581 ccgataggtt tacccacgaa gggtaatggc aactcgattg attaaattta cgccgcatct
    17641 gcgctcctat accagatagt gtcggacagg gtattgagaa aggacaaggt cgctttccta
    17701 tcaccagcca tgcccgagga caggctatga gggacatgcc atcacaaggc gcaataacga
    17761 ttctggggcg cctgccgatc gcgatcgaag gattgaaaag aatactcacg gaatatggtt
    17821 ttgacgtgat cggctctggt atggatattg cctccatccc aaaatctatt gtccaagagg
    17881 gcgattttca tatcgtcatc gtcgatgaat tgatcgatgt tcacggccct aaatatgtgg
    17941 gggaagtgct gaaaacgttt cgcgacgctc gaatagtgat cgcgtcaaac gcgttcgatt
    18001 ttgccacgat ggcggcctgt ttccgtgaag gagcttttgg ctacattgtg aagagcatct
    18061 cgtcgaaatc gctcgtcgca tcgctgcagc tggttgcggc gggagagaag gtgatgccga
    18121 cgagcttggt cgacgcgtta ccggatcacc cggggggcgg gtacgatagt ggctacacca
    18181 gtccatcctt tcgggatgtt ggattgtcgc agcgcgaggc tgagatactc acctatctgg
    18241 tcaatggtca gcccaataag aagatatcgc gcgatctcaa gattagtgaa gcgactgtca
    18301 aagtccatgt aaaggcaatt ctgcggaagg ttggcgtgca gaaccgtacc caggcggcga
    18361 tttgggcaat gggtgaaaag gaagacagcg ccaatacggg agagggggaa ccgtcccaca
    18421 acgggcaccc ctgagggcgt cgggtggggc gtatcatgtc atggtggcgt ataggtgtac
    18481 ggcgaggtgc gagacgaacc acggtcctgc ggtattcaat ccgcgcatca gagtctgatc
    18541 agccgtcgtc ttactgccgc cgcgtccgac cctgcatctc ttcatcttac ttcacgccaa
    18601 aagaggcgat cggcgaccta cgcgctctct tgaattcgga catgagaatc agcactggtt
    18661 catgatgcac gccgtcagtt acgaaggccg cctaatgaca accatgatgg ccggttcgtg
    18721 aagagttgat cggcatgttc cc
//
LOCUS       DATGEM010000390         6844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_273928, whole genome shotgun sequence.
ACCESSION   DATGEM010000390 DATGEM010000000
VERSION     DATGEM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273928"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1561
                     /locus_tag="VKQ27_12420"
     CDS             206..1561
                     /locus_tag="VKQ27_12420"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLY89779.1"
                     /translation="MGKVASAMWAMLGGAICGAGLLSSVSAFADSTTDCHVGSYRLAS
                     GAAVDIAPSEGDTLRWRLFTGETGQLHRQADGTWKSTFGWTDRLDGKTVSFSDCEKGE
                     VTFGADVGHRIAFDTIETTFVSNGVKLVGRLVMPKGKDKAPVVVLVHGSEHDSARDFY
                     SLQRMFPAQGIGAFVYDKRGTGASGGVYTQDFDILADDAVRALEEARRLGGARVGRSG
                     YQGGSEGGWVAPLAANKARVDFTIVSFGLAVTILEEDQQSVALDMHFHHHEADTPKAL
                     ELARAGERVIETGGKEGYAEFDALRQKYRNEPWYKDVHGDFVFMVLPLDKAQIDEAVK
                     QFDFHTPFRYEPMLALRSSTTPQLWISGTDDLEAPSAETARRIKSLIADGRDYTFALY
                     PGAEHGMTEYELNATGERISTRYAPGYFQMMADFIRNGRIGAHYGAADITQPSPRSGA
                     P"
     gene            1558..2895
                     /locus_tag="VKQ27_12425"
     CDS             1558..2895
                     /locus_tag="VKQ27_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY89780.1"
                     /translation="MSAPADPRHGGARRWRFVEVPMRGRLIGLGLQVCLILPWLSPSE
                     ARADTSRDAVVAVAQCRGGKKVALDVQILACSRILNTGGESREYAVWALNERASARID
                     KGDVDGALSDIGIALQLEPESPVTFANRGRAYNAKGDHNRAISDYDKAIEINRDFYPA
                     YFDRAIAFEQMGNPDRAIADFDKVIALKPNDPAGYRERGDMWMIKNDTDRALADLDHA
                     LEIKPDYEPAFGSRGDVWLKRKEWGKAISDFDQALRLKPDSPARIPPLYYARGVAWVE
                     SGDLDRGIADLDESLRLRPDNVLTLEARSSALQRKGQLDKALVDLDKAIRLEPDNGYA
                     LNGRCFVRGLRNRDLDDALADCDRAIHLLPGQPAILDSRAFLRFRRAEYAQAIADTDA
                     SLAVDPKFADAMFVRGLAEKALGRISAAAADLAAARAADPTVGDVYASSGVVP"
     gene            complement(3363..3530)
                     /locus_tag="VKQ27_12430"
     CDS             complement(3363..3530)
                     /locus_tag="VKQ27_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89781.1"
                     /translation="MKKPTAPLPPKTQGEKFIDLARELECDEDEEAFDERLRRIAKEP
                     KLAKEKPDGER"
     gene            complement(3475..4269)
                     /locus_tag="VKQ27_12435"
     CDS             complement(3475..4269)
                     /locus_tag="VKQ27_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89782.1"
                     /translation="MPLGEAIELIKKLHANNRTNPVDREAAVKEMGYASLSGRSAKVL
                     SDLSHFGLLEKSGTGSVRVARRAVEILYPESGESQQQALHEAAGTPSLFAELNEHFDD
                     GLPSENALRSYLMRKSFASAAIPPVIKSYLETYRSLEQQNVSGGHRQSDENPGESKGS
                     KIDAHPTGGGADHTRQKTPADGGQPKREVTLMDGERVIFVEEVGAGQYLKLVATGQLD
                     DSLLEALEDFTKRQRKRLGAPSKPKDPPSEEANGTTAAEDAGGKVH"
     gene            complement(4718..5171)
                     /locus_tag="VKQ27_12440"
                     /pseudo
     CDS             complement(4718..5171)
                     /locus_tag="VKQ27_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            5237..5449
                     /locus_tag="VKQ27_12445"
                     /pseudo
     CDS             5237..5449
                     /locus_tag="VKQ27_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5669..6268
                     /locus_tag="VKQ27_12450"
     CDS             5669..6268
                     /locus_tag="VKQ27_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89783.1"
                     /translation="MSESLLVAGVVCIVAAIVGGGIKLLGAEVPVLNSFPRQALLFVV
                     GAGFLLASFRVGANRSPTPSPPSPAQAAAQAPAQTPTSASVQAPPSAPSGAVAPCGAA
                     AALSCLPRSSSPVVFVDEAAANRAASQNIQDSLSTTQAQAEAIREGASGKASRLCGIV
                     GSNDSSPLGKHLAAIRLNNPQPADGQSTPACLKLAQSFF"
     gene            complement(6312..6518)
                     /locus_tag="VKQ27_12455"
     CDS             complement(6312..6518)
                     /locus_tag="VKQ27_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HLY89784.1"
                     /translation="MPDHVYKIVEIVGSSETSIEEAINSAVARASKTLRNLRWFEVVQ
                     TRGEIRDGKAHFFQVELRVGFTME"
BASE COUNT         1239 a         2176 c         2129 g         1300 t
ORIGIN      
        1 ccgctcttcc gcgccgaggc cgaactgctc gcgccctcga ccctgaccgt cggcgccgtc
       61 gccgacgccc tcgagcgcct ctccgccgag atcatggtcg acatcaccct ggaggagcgt
      121 cgatcgtagg cgaaaggtgg agaaggttta acgggacagc cgctcgccga acagcgagcg
      181 tggtcgccgc cgaagatcac gaaaggtggg caaggtggcg agcgcgatgt gggccatgct
      241 cggcggggcg atctgtggcg ccgggctgct gtcgtccgta tcggcgttcg cggattccac
      301 aacggactgt catgtggggt cgtatcgact ggcgagcggc gcggcggtgg atatcgcccc
      361 ctcggaggga gacacgctgc gctggcgact gttcaccggc gaaaccgggc agcttcacag
      421 gcaggccgac gggacctgga agagcacgtt cggctggacc gatcggctgg acggcaagac
      481 tgtctccttc tccgactgcg agaagggcga agtcacgttc ggcgcggacg tgggccatcg
      541 catcgccttc gacaccatcg agacgacgtt cgtcagcaac ggcgtgaaac tggtcggccg
      601 cctcgtcatg cccaagggca aggacaaggc gcccgtggtg gttctggtgc acggttcgga
      661 gcacgactcc gcgcgagatt tctattcgct gcagcgcatg ttcccggccc agggcatcgg
      721 cgcgttcgtc tatgacaaac ggggcactgg cgcgtctggc ggggtctata cgcaggactt
      781 cgacatcctc gccgatgacg cggtgcgcgc cctggaagag gcgcgcaggc tcggcggcgc
      841 ccgcgtcggc cgcagcggct atcagggcgg cagcgaaggg ggatgggtgg cgccgctggc
      901 ggcgaacaag gcgagggtgg acttcaccat cgtcagcttc ggcctggccg tcaccatcct
      961 cgaggaagac cagcagagcg tggcgctcga catgcacttt catcatcacg aggccgatac
     1021 gcccaaggcg ctggagctgg cccgcgccgg cgagcgggtc atcgagaccg gcggcaagga
     1081 gggctatgcc gagttcgacg ccttgcggca aaagtatagg aacgagccct ggtacaagga
     1141 cgtgcacggc gatttcgtgt tcatggttct gcccctcgac aaggcgcaga tcgacgaggc
     1201 cgtgaagcag ttcgatttcc acacgccgtt ccgctatgag ccgatgctgg cgctccgaag
     1261 ctcgacgacg ccgcaactgt ggatttcagg taccgacgat ctcgaggcgc cgagcgcgga
     1321 gaccgccagg cgcatcaagt cgctgatcgc ggacggcaga gactacacgt tcgccctgta
     1381 ccccggcgcc gaacacggca tgaccgaata tgagctgaac gcgaccggcg aacggatctc
     1441 gacgcgctac gcgccgggct acttccagat gatggccgac ttcatccgca acggccggat
     1501 cggcgcccac tatggcgcag cggacatcac gcaaccctcg ccgcgttcag gcgcgccttg
     1561 agcgcaccgg ccgatccgcg gcatggtgga gcgcggcgtt ggcgatttgt cgaggttccg
     1621 atgcgtggcc ggttgatcgg tcttggcctg caagtctgcc tgatccttcc atggctgtcg
     1681 ccctctgaag cgcgggccga cacctcacgc gacgccgtcg tcgccgtcgc ccaatgccgt
     1741 ggcggaaaaa aagtggcgct cgatgtgcag attctagctt gttccaggat cctcaacacg
     1801 ggtggcgagt cgagggagta cgccgtctgg gccctgaacg agcgtgcaag tgcgcggatc
     1861 gacaagggcg atgtcgatgg agcgctctcc gacatcggca tcgcgctcca gctagagccg
     1921 gaatcccccg tgacgttcgc taaccgtggt cgtgcgtaca acgccaaggg cgaccacaac
     1981 cgcgcgatct ccgattacga caaagccatc gagatcaatc gcgatttcta tccggcgtat
     2041 ttcgaccggg cgatcgcatt cgagcagatg gggaacccgg atcgagcgat cgccgatttc
     2101 gacaaggtca tagcgctcaa gccaaacgac ccggccggct acagagagcg gggtgacatg
     2161 tggatgatca agaatgatac ggaccgcgcg ctcgccgacc tcgaccacgc cctcgaaatc
     2221 aaacccgatt acgaacccgc tttcggcagc cgaggtgacg tctggttgaa gaggaaggag
     2281 tggggcaaag ccatttcgga ctttgaccag gcgctccgac tgaagccgga ttcgccggcg
     2341 cgaatcccgc ccctctacta cgccagaggc gttgcatggg tggaaagcgg ggatttggat
     2401 cgaggtattg ctgatctcga cgagtctctg cggcttaggc cagacaatgt actgaccctt
     2461 gaggcccgca gttcagcttt gcagcgaaag ggtcagttgg acaaagcctt ggtcgatcta
     2521 gacaaggcaa tacggctgga gccagacaac ggatatgctc tgaacggccg gtgttttgtt
     2581 cgcggcctcc gcaaccgcga cctcgatgac gctcttgccg attgtgatcg cgccatccat
     2641 ctgcttcctg gccaacctgc cattctggac agtcgagcgt tcttgcggtt ccgacgcgcc
     2701 gaatacgcac aggctatcgc ggataccgac gcatccctcg cagtcgatcc caagttcgcg
     2761 gacgcaatgt tcgtacgcgg attggccgag aaggccttgg gcagaatttc ggcggcggcc
     2821 gccgatctag ctgccgcgcg cgcggcggat ccgaccgtcg gcgacgtcta tgcgagttcc
     2881 ggcgtcgttc catgaatcgc cgaggcgact atgtcttttg caaacccgta gggcgccttc
     2941 ttctacgcca ggtccaccat ccaatcgcga catagccggc gaacgccaca gccatcccga
     3001 ttaacagaat ttctctcttc tccgctgcgc aagcagccac gatatccctc ggaccagcgc
     3061 aatcgccatc gacagttagg ataagcccga gcaggaaaag agcggcgaac cagtaagcaa
     3121 gcgtgaggag aatggcccgg atagtggcct tggctatcgt caatctgatt aatcatcctc
     3181 cggtgacgtc tgagccatcg tagccgcgtg ggcctttgcg gcttcccggc tagccttagc
     3241 ctctgctgag gctcggtcag cttcactagg ctgggtatag gaccttatgc gggagtagat
     3301 caggaagacc gacacggcgg caaatagaac gagaaagagt atctgaccgg ccgcgtggag
     3361 ggtcaccgtt cgccatccgg tttttccttc gcgagcttcg gctccttagc gatccgcctc
     3421 aggcgctcgt cgaaggcttc ctcgtcctcg tcgcattcca gttcgcgcgc gaggtcaatg
     3481 aacttttccc cctgcgtctt cggcggcagt ggtgccgttg gcttcttcac ttggcgggtc
     3541 cttcggtttt gacggtgccc caaggcgctt gcgctggcgc ttcgtaaagt cctccagcgc
     3601 ctccaacagg ctatcgtcca actggcctgt ggcaaccagt ttgagatact gcccggctcc
     3661 gacctcctct acaaagatca ctcgctctcc gtccatcagg gtaacctctc gcttaggctg
     3721 ccctccgtca gcgggagttt tctggcgcgt atgatccgcg ccgcctcctg tggggtgagc
     3781 atctatcttg gaaccctttg attctccggg attttcatct gattgtcgat gaccgccgct
     3841 tacattctgt tgctcaagag atcggtaagt ttctaggtag ctctttatga cgggggggat
     3901 cgccgcgctg gcgaatgact tgcgcataag gtaggagcgc aaagcgtttt cagatggcaa
     3961 tccgtcgtca aaatgctcgt taagctccgc gaacagcgac ggcgtgccag ctgcttcgtg
     4021 cagcgcctgc tgctgagatt cacccgattc ggggtagagg atttcgacag cacgccgcgc
     4081 gactcgaact gagccggttc ccgatttctc caaaagtcca aaatgggaaa gatcggatag
     4141 gaccttggcg gaacggccgc taaggctcgc gtatcccatt tccttgaccg ccgcttcacg
     4201 atccaccggg tttgtccgat tattggcgtg aagtttcttt atcaattcaa tagcttctcc
     4261 gagcggcatg ctcggatagc cagggcttcg ggtaacggcc atctctgatg ctccttgtgt
     4321 gttgcccgcc aggagtaaca aaaactgcgc gtccgcgcaa gattttcttg cgtgggatac
     4381 cttactggtt gacggcgtcg gagtaaggcg gataaatatc gacgccgtcc tcatcacggc
     4441 agaaaggttg gcggttatga ccaggagggt cgccctcgcc gcgtcaaagc tcgcgcgaac
     4501 gcggtagggg ttaaacgaga gaggccgccg cccaggcccg ggcgacgacc tcaatgtgag
     4561 ccaagatcgg agtgaccgga actggcggtc gtgcccatag cacggccgcc tttcttttgg
     4621 aatcccattg tcttcaaagg gtaatttcag ctgatccgac tcggatgccg ccaccatctc
     4681 aggaccgccg ccatggcggc gacgacgccc acgcggctta tgcttcgggc cgctcaccag
     4741 tcctgcaata cgtcaggcgc ttgccgaccg cccccaacag cgccgtatga gcccccgcgg
     4801 tgtcgtcgac tcccagcgcg acacggttcg aatagcggaa gtcgaactcg gcgaggtagc
     4861 ggtgaaggtg cttgcctcgg cagtgctgat agacgccggt catgccgcgc ttgaagatcg
     4921 agtagaagcc ttcgacggtg ttggtgtgaa tggtcttgtc ctgccggttc acgtactggc
     4981 ccttgccgtg ggttgacgga gacgtgatcc gcgaaggcgc ggcccgggaa cttgtaagcg
     5041 ccagcctcat cagtgattag ccgggccttg ggcgacaccg ccaccgacag cacgtcatga
     5101 accacgtcca tgcggaattg ggtgatgact tcgctgtagg cgcggccggt atcgcggtcg
     5161 atcaggctca tgactgcgta cttgtgatgc gggccgccct tgaactccac gccgggcctg
     5221 taagggaatc aacgccatgt ccattctttc cgccccccac tttcacgacg aagccgccgc
     5281 cttcgcgaag gtggaagccg ccctttggcc gaatggtccc gcgtgctttc actgtggcgc
     5341 ggccgaccgc atcagcgcca tcaagccgaa ccccgccaag cgcgtccggt ttggtctgaa
     5401 gaagtgcggc cactgcaaga agcagttcac cgttcgcatg ggcacgatct caagccgtga
     5461 ccagtctcgc gcagcttcgg gagaaactgc tgaccggccc ccgcatggac ggctcccaat
     5521 ctgtcgtcgg atgcctggac agaagcacct gtccctgggg gcgcctgggg gcgcatcgct
     5581 ccacccgcct atgtcggcgc gcccctggac gagaccctga acttgtgtcg ccattgctgg
     5641 atcagggtca gggccgaggt gcgccggggt gtcggaaagt ctgttggtcg ctggggtggt
     5701 ctgcatcgtc gccgccattg tcgggggcgg cataaagctg ctcggcgcgg aagtgcccgt
     5761 gctcaactcc tttccccgcc aggcgctgct gttcgtcgtt ggcgcggggt ttctgctcgc
     5821 cagcttccgg gtcggcgcca acaggtctcc aacgccttcg ccgccatcgc ccgcgcaggc
     5881 agcggctcag gctcctgcac aaacgcccac atccgcgtcc gtccaggctc cgccgtcggc
     5941 tccctctggc gccgtcgcgc cttgcggtgc ggcggcggcc ctgtcgtgtc tgccaaggag
     6001 ctcgagcccg gtggtgttcg tcgacgaggc cgccgccaac cgcgccgcga gccagaatat
     6061 ccaggacagc ctcagcacca cccaggccca ggccgaggcg atccgggagg gcgccagcgg
     6121 caaggcgtcc cggctgtgcg gcatcgtcgg ctccaacgac tccagcccgc tgggcaagca
     6181 cctcgccgcg atcaggctga acaatccgca gcccgcggat ggccagtcca ccccggcgtg
     6241 cctgaagctg gcgcagagct tcttctaggc cgggccccgg gcgatcccgt gttcggcagg
     6301 cccgatacag cctactccat cgtgaagcct accctcagct caacctggaa gaaatgggct
     6361 tttccatccc tgatctcgcc acgtgtctgg accacctcga accatcgcag gttgcgaagc
     6421 gtcttgctcg ctcgggcgac ggcgctgttg atcgcctctt ctatgcttgt ttccgatgag
     6481 ccaacgatct caacgatctt atagacgtga tccggcatgg cagcctcctc gtccgcttgc
     6541 gtcccttgat actgcatccg tagctcacgc gggtccaccc cgacgttcgg acacgccacg
     6601 gacgcatccc gacccaggcc acggggaatg gaaggcgggg tccgtcggga acgtcgtcct
     6661 gataaacagc cgtcagtcga tgccgccgcc gggagaatcg cttatgaaac gcatctggtc
     6721 ccttgcggcc cttaccctcg tcgccgcctt tgccgccggc gcggcgttcg ccttgccgaa
     6781 gttcgaggac cactatgcct cgaacgggac cgtgaagatc cactacgtcg ccgccggttc
     6841 cagc
//
LOCUS       DATGEM010000391         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_796870, whole genome shotgun sequence.
ACCESSION   DATGEM010000391 DATGEM010000000
VERSION     DATGEM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796870"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /gene="gcvP"
                     /locus_tag="VKQ27_12460"
     CDS             <1..1827
                     /gene="gcvP"
                     /locus_tag="VKQ27_12460"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HLY89785.1"
                     /translation="IARRVASYTAVLASGLRRLGCTLAHESAFDTLRVTTGAKTEILM
                     QAACDAGYNLRRASATSVGITLDETTTRADLASLWKVFADQQALPDIGQFEQGIASLI
                     PPALERTSAFLTHPVFNRHHSETNMLRYLRSLADKDLALDRTMIPLGSCTMKLNAAAE
                     MIPITWPEFAGLHPFAPRDQLAGYDTLRAHLEAWLCEATGYSGVSLQPNAGSQGEYAG
                     LLIIKAWHDSRGDHQRNICLIPESAHGTNPASAHMAGMQVVVTKCDAGGNVDLADLKA
                     RCEQYSARLGAVMITYPSTYGVFDPQVKALCALVHSHGGRVYVDGANLNALVGLAAPG
                     EFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVAADLAPFLPAHRSAGLGTDRQVGAISA
                     APLGNAGVLPISWMYMRMMGAQGLQAATETAILSANYIAARLADDYELGFSGGIAGIK
                     GGGVAHECILDLRPLKDSTGGAEGISAEDVAKRLIDYGFHAPTLSFPVAGTLMVEPTE
                     SESLQELDRFCDAMIAIRAEIAKVESGQWPRADNPLKNAPHTAETLLKSDWPHPYSRE
                     EAAYPRASLRKAKYWSPVGRVDNAWGDRNLFCSCPPLGRYET"
     gene            complement(1930..>2516)
                     /locus_tag="VKQ27_12465"
     CDS             complement(1930..>2516)
                     /locus_tag="VKQ27_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HLY89786.1"
                     /translation="FLVLLSACAAVRADHYRWIDADGKVNYSDQPPPANIKQVERIKS
                     AGGKPGDAPLPYALQQAVTNFPVTLFVSECGDACSRARELLAKRGIPYTEMDATDPAT
                     QEELKKLTGGAIQVPVLKVGSDTTKGFEEGKWNTSLDAAGYPQTAVIAPRPPTKPPKP
                     AAAPPPPAAVAPPAAAVPPAEQPATPSPPAEATQ"
BASE COUNT          459 a          802 c          819 g          436 t
ORIGIN      
        1 attgcgcggc gcgtggccag ctatacggca gtgctggcgt cgggattgcg gcgcctcggc
       61 tgcacgctgg cgcacgaaag cgccttcgac acgcttcggg tgacgaccgg cgcgaagacc
      121 gaaatcctga tgcaggcggc gtgcgacgcg ggctacaacc tgcgccgcgc gtcggcgacg
      181 agtgtcggca tcacgctcga cgagacgacg acgcgcgcgg accttgcgtc actgtggaag
      241 gtgtttgcgg atcagcaggc cctgcccgat attgggcagt tcgaacaagg catcgcctcg
      301 ctgatcccgc ccgcgctgga acgcaccagc gccttcctca cgcatccggt gttcaaccgc
      361 catcacagcg agaccaacat gctgcgctac ctgcgcagtc tggccgacaa ggatctcgcg
      421 ctcgatcgca cgatgatccc gctgggctcg tgcaccatga agctcaatgc cgcagccgag
      481 atgattccga tcacctggcc ggaattcgcc ggcctgcacc cgttcgcgcc gcgcgatcaa
      541 ctcgcgggat acgacacttt gcgcgcgcat ctcgaagcct ggctgtgtga ggccaccggc
      601 tacagcggcg tgagcctgca acccaacgcc ggctcgcagg gggaatacgc cggtctgttg
      661 atcatcaagg cctggcacga ctcgcgcggc gaccatcagc gcaacatctg cctgattccc
      721 gagtcggcgc atggcaccaa cccggccagc gcgcatatgg cgggcatgca agtggtggtg
      781 acgaagtgcg atgccggggg caacgtcgat ctcgcggacc tgaaagcccg gtgcgagcag
      841 tacagtgcgc gcctcggcgc ggtgatgatc acttaccctt ccacctacgg cgtattcgac
      901 ccgcaggtga aggcgttgtg cgcgctggtc cacagccatg ggggtcgcgt ctatgtcgat
      961 ggtgcgaacc tgaacgcact ggtcgggctg gcggcgccgg gcgaattcgg cggcgatgtg
     1021 agccacctca acctgcacaa aaccttctgc attccgcatg gcggcggcgg cccgggcgtc
     1081 gggccagtgt gcgtggcggc ggatctggcg cccttccttc cggcacatcg cagtgcgggc
     1141 ctcggaacgg accggcaagt gggcgccatc agcgcggcgc cgctcggcaa tgcaggagtg
     1201 ctgccgatca gttggatgta catgcgcatg atgggcgcgc agggattgca ggcggcgacc
     1261 gagacggcga tcctgtcggc caactacatc gccgcgcgcc tggctgacga ctacgagctg
     1321 gggttcagcg gaggcatcgc cggcattaaa ggcggcggcg tggcgcacga atgcatcctc
     1381 gacctgcggc ccctgaagga cagcaccggc ggcgccgagg gcatcagcgc ggaagacgtg
     1441 gccaaacgcc tgatcgacta tggctttcac gccccgacac tgagttttcc ggtggccggc
     1501 acgttgatgg tcgaacccac cgaaagcgaa tcgctgcagg agctggaccg tttttgcgac
     1561 gcgatgatcg ccatccgcgc cgaaatcgcc aaggtcgagt ccggtcaatg gccgcgggcc
     1621 gacaacccgc tgaagaacgc gccgcacacg gccgaaacct tgctcaagag cgactggccg
     1681 catccctact cgcgcgaaga agccgcctac ccgcgcgcaa gtctacgcaa ggccaagtac
     1741 tggtcgcccg tgggtcgggt ggacaacgcc tggggcgacc gcaacctgtt ctgttcctgc
     1801 ccgccgcttg gccgctacga gacttaaccc gggttaaccc gacttattat cgaaatctcg
     1861 aggctggata agatcgcctt agaggtctct gaaatcttcc aaaggtcgca agcgacgagc
     1921 ttcgaggaac tattgcgtag cttccgcggg aggcgaaggt gtcgccggtt gctcggctgg
     1981 tggaacggcg gcggccggtg gggcgacggc ggccggtggg ggtggggcag cggccggctt
     2041 gggcggtttg gtcggcggac gcggagcaat cacagccgtt tgtggataac cagcagcgtc
     2101 cagcgacgta ttccacttcc cctcctcgaa gcctttggtc gtatcactgc caactttcag
     2161 aaccggaacc tggatagcgc cccccgtgag ttttttcaac tcttcctggg tcgctggatc
     2221 cgttgcatcc atctcggtat acggaatgcc gcgctttgcg agcagttcac gcgcgcgcga
     2281 acaagcgtcc ccgcattcgc tgacaaacaa cgttaccgga aaattcgtga ccgcctgctg
     2341 gagcgcataa ggcaagggag catcaccggg ttttccaccc gccgatttta tcctctcgac
     2401 ctgcttgatg ttggcgggcg gaggttgatc gctgtagttg accttgccgt ccgcatctat
     2461 ccagcggtag tgatcggctc gcacggcggc gcaggccgat aacagaacca gaaaca
//
LOCUS       DATGEM010000392         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1371412, whole genome shotgun sequence.
ACCESSION   DATGEM010000392 DATGEM010000000
VERSION     DATGEM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371412"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1028
                     /locus_tag="VKQ27_12470"
     CDS             147..1028
                     /locus_tag="VKQ27_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY89787.1"
                     /translation="MNASLDQDAASFEAHRRALTGLAYRMLGSRAEAEDVVQDAYLRW
                     HAADHAAVEEPRRYLGTVVTRLCLDRLKSARAKHEEYVGPWLPEPVVDEVFDEPSAGE
                     LAHDISVALMLLLERLSPLERASFLLHDVFGLDFSEVAGALGRGEAACRQLAVRARAH
                     IEAGRPRFPASREEGHRLAVAFQVAVASGDTEGLMQILAEDAVLYSDGGGKRAAALNP
                     IFGADRIVRFFAGVLRKNSTLASVVARSAVVNGLAGFVLREEDGSIDTMAIDHRDGRI
                     VGIYLTRNPEKLRHVRF"
     gene            complement(1059..1919)
                     /locus_tag="VKQ27_12475"
     CDS             complement(1059..1919)
                     /locus_tag="VKQ27_12475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY89788.1"
                     /translation="MRNTPDMSPDSTAARVTLWRALHFELDPTPHVIEDVIGLRLLAP
                     NADWRRRGDMDPEFTRPFRASIVARARFIDDLVGEQVRRGTTQYVILGAGLDSFAQRR
                     SDLTSHLTIFEVDQPGPQSWKRRRLIELGFGVPDWLRFISTDFAAGEPWYGNLAGTGF
                     DYGKPAIVVSTGVSMYLTKDANAATLRQIATLAAGSILAMTFLLPVELAAAEARPGLE
                     MALKGARASGTPFLSFFTPPEMLALAGEAGLGNPRHVSAADLAQRCFAARTDGIRPPN
                     NAEEFLVAST"
     gene            2022..3008
                     /locus_tag="VKQ27_12480"
     CDS             2022..3008
                     /locus_tag="VKQ27_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89789.1"
                     /translation="MKRNTWGRAVLLLGLALLSIAGGKAALADTVTIAHSTWVGYGPL
                     YIARDKGFFKQNGVDVDLVVMEDPKERFPTLMADRIQMIASTVDTALLYLKKPTDFQY
                     VVAIDDSNGGDGIVAKKDITSVADLKGKHVGVSEGSVSEFYLNVLLSKAGLKESDLNT
                     VNMTAADAGGAFVAGRVDAAVTWEPWLSRGKATSFGHLLTDSSKTPGLITDVLIVKTD
                     WAKSHQKEIAAIVKSWNEAVAFYEQHPDEAIPIMAKGVGGWLKDPKDFKDTLSGIKFY
                     DEAANKVFFGTSGKPGPLTNTVQQAIDIWGSHGKLQVKATPAELISYSYINQ"
     gene            3008..3808
                     /locus_tag="VKQ27_12485"
     CDS             3008..3808
                     /locus_tag="VKQ27_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89790.1"
                     /translation="MASFLDTYVAPKKEIPRHTYLAVSFVVTATILAVWCGLSYGGLV
                     REDFLPTPGAVAGAAIAGLGDGSLAVNTAVSVTEIMSGFIIASVFAVPLGILMGSFKV
                     VEAAIEPVTNFVRYLPVSALIPLLILWVGIDITEKIAVIFIGTFFQQLILIADVSRGV
                     SQDLLDVSYTLGATRRDVVARVLIPATMPGVMDTLRVTLGWAWTYLVVAELVAANKGL
                     GYFILNSMRGLFTEQIFLGIMVIGLLGLITDQLFKTLRNWMMPWAASP"
BASE COUNT          690 a         1076 c         1354 g          742 t
ORIGIN      
        1 ctcgctttcg ccatgctcgc ggggcggatc tatccaacgg tgaaatatgc gctgggccac
       61 ggccatgcct gcatgcggct gaccatcggc ggggagaccc ggccggtgct gcgcgaggtg
      121 acgcagccgc agcgggctgc cgcggcatga acgccagcct cgaccaggac gccgcttcgt
      181 tcgaggcgca tcggcgcgcg ctgaccggcc tggcttatcg catgctgggc tcgcgcgccg
      241 aggccgagga cgtggtgcag gatgcgtatc tacgctggca cgctgcggat catgcggcgg
      301 tcgaggagcc acgccgctac ctgggcacag tggtgacgcg gctgtgcctc gaccggttga
      361 agtcggcgcg cgccaagcat gaggaatatg tcgggccttg gctgccggag cccgtggtcg
      421 atgaagtgtt cgacgagccc tcggcgggcg agctggcgca tgacatctcg gtggcgctga
      481 tgctgctgct ggaacggctg tcgccattgg agcgggcgag tttcctgctg catgacgtgt
      541 tcgggctgga tttttccgaa gtggcgggcg cgctggggcg gggcgaggcg gcgtgccgcc
      601 agcttgcggt gcgggcgcgc gcgcatatcg aggcggggcg accgcggttt cctgcatcgc
      661 gcgaggaggg ccatcggctg gccgtcgcct tccaggtggc ggtggcgtcg ggcgataccg
      721 aagggctgat gcagatactg gctgaggatg ctgtgctgta ttcggacggc ggcggcaagc
      781 gcgcggcagc actcaatccg atcttcggcg ccgatcggat cgtgcggttc tttgctggcg
      841 tgctgcggaa gaattcgaca ctggccagcg ttgtcgcgcg gtccgccgtg gtgaacgggc
      901 tggcgggctt cgtgctgcgc gaggaggacg gatcgatcga cacgatggcg atcgatcacc
      961 gtgatgggcg gattgtcggg atctacctga cgcgcaatcc ggagaagctg cggcacgtaa
     1021 ggttctgagc gaactgtggt ggtgccgcgc cccggtattc aagtggacgc aaccagaaac
     1081 tcctcggcgt tgtttggcgg gcgaatccca tccgttctgg ccgcaaagca gcgctgcgca
     1141 aggtccgctg ctgacacgtg ccgtgggttt cccagcccgg cttcgccggc tagcgccagc
     1201 atctccggcg gcgtgaagaa gctgaggaat ggtgtgccgc tggcgcgtgc tcccttcagc
     1261 gccatctcga gtccgggacg cgcctcggcg gctgccaact caacgggaag caggaatgtc
     1321 atggcaagga tcgatcctgc cgcgagcgtg gcgatctgtc gcagcgtggc cgcgtttgcg
     1381 tccttggtga gatacatgct cacgccagtg gagaccacga tcgccggctt gccgtaatcg
     1441 aagccggtac cagcgaggtt gccgtaccag ggctcgcccg ccgcgaagtc cgtcgatatg
     1501 aagcgcagcc agtcggggac gccgaagcca agctcgatca ggcgccggcg cttccatgac
     1561 tgtggaccgg gttgatcgac ctcgaagatc gtgaggtgcg aggtcaaatc cgaccttcgc
     1621 tgtgcgaagc tgtcgagacc agcaccgagg atgacgtatt gggtcgttcc ccggcggacc
     1681 tgttccccaa caagatcgtc gatgaagcgg gcgcgggcca caatcgaggc gcgaaagggc
     1741 cgggtgaatt ctggatccat atctccgcgg cgacgccaat cggcgtttgg agccaacaat
     1801 ctcagcccga tcacatcctc gatcacgtgt ggagtcggat cgagctcgaa atgcaaagcg
     1861 cgccatagcg tgacccgcgc agcggtgctg tctggcgaca tatctggcgt attgcgcatt
     1921 gcgccaacat ggaccgcctg cggttatttt tgctatggca aattcggagg cgttgcgggc
     1981 ggcgcatggt gtcgcgatgc aggctgacga gggagggaac tatgaaacgg aatacctggg
     2041 gcagggctgt cctgctattg ggactggctc ttctgtcaat cgcgggaggg aaagccgcgc
     2101 tggccgatac cgtgacgatc gcccactcca cctgggtcgg ctacgggccg ctgtacattg
     2161 cccgcgacaa gggcttcttc aagcagaacg gcgtcgatgt tgacctggtg gtcatggagg
     2221 atccgaagga gcggtttccc acgctgatgg ctgacaggat ccagatgatc gccagcacgg
     2281 tggatacggc gctgctgtat ctcaagaagc ccactgactt tcagtatgtg gtggcgatcg
     2341 acgattcgaa cgggggcgat ggaatcgtcg caaagaagga catcaccagt gttgctgatc
     2401 tcaagggcaa gcatgtgggg gtcagcgaag gttcggtctc ggagttctat ctgaacgtgt
     2461 tgctgagcaa ggccgggctg aaggaaagcg acctcaacac ggtgaacatg acggccgcgg
     2521 atgcgggtgg agcgttcgtt gcgggccggg ttgatgccgc ggtgacatgg gaaccatggc
     2581 tgagccgcgg caaggcgacc tcgttcggac atctgctgac ggacagttcg aagacgcctg
     2641 gattgatcac cgacgtgctg atcgtgaaga cggactgggc caagtcacac cagaaggaaa
     2701 tcgctgcgat cgtgaagtcc tggaacgagg cggtggcgtt ctacgagcag catcctgacg
     2761 aggcgatccc gatcatggcg aagggcgtcg gcggttggct gaaggatccg aaagacttca
     2821 aggacacgct gtcggggatc aaattctatg acgaggcggc caacaaggtg ttcttcggca
     2881 cgtccggtaa gccggggcca ctgaccaata ccgtgcagca ggcgatcgac atctggggca
     2941 gccacgggaa gctgcaggta aaggcgacac ccgccgaatt gatcagctac agctacatca
     3001 accagtaatg gcgtcatttc tcgacacgta tgtggcgccc aagaaggaga tcccgcgcca
     3061 cacttacctg gctgtcagct tcgtggtcac agcgaccatc ctggcggtgt ggtgcgggct
     3121 ttcctatggc gggctggtgc gggaggattt cctcccgaca ccgggtgcgg tcgctggcgc
     3181 cgcgatagcg ggattgggtg atggatcgct ggcggtcaac acggctgtga gcgtcaccga
     3241 gatcatgtct ggcttcatca ttgcatcggt attcgcggtg ccgcttggca ttctcatggg
     3301 cagcttcaaa gtcgtcgagg ccgcgatcga gccggtgacc aatttcgttc gctatctgcc
     3361 ggtgtcggca ttgattccgc tgttgatcct gtgggtgggg atcgacatca ccgagaagat
     3421 cgcggtgatc ttcatcggca cgttcttcca gcaactgatc ctgatcgccg acgtgtcgcg
     3481 gggcgtttcg caggatctgc tggatgtctc gtatacgctg ggagcgacac gccgcgatgt
     3541 ggtggcgcgt gtattgatcc cggcgacgat gccgggcgtg atggacacgc tgcgcgtcac
     3601 gctggggtgg gcatggacgt atctcgtggt tgctgagctg gtggcggcca acaaggggct
     3661 tggctacttc atcctcaatt cgatgcgggg cctgttcacc gaacagatat tcctgggcat
     3721 catggtgatc ggtttgctgg gactgattac cgaccagctc ttcaagacgc tgcgaaactg
     3781 gatgatgcca tgggcggcga gcccgtgatg ttgcagcagg tcgagggggc ggcgaccgcc
     3841 ggtgcaaagc tgcggatcga ta
//
LOCUS       DATGEM010000393         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_137516, whole genome shotgun sequence.
ACCESSION   DATGEM010000393 DATGEM010000000
VERSION     DATGEM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137516"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VKQ27_12490"
     CDS             <1..510
                     /locus_tag="VKQ27_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLY89791.1"
                     /translation="EIELVGLDNREFRLREALAPLRAAPRFSFVLIDCPPSLGLLTLN
                     GLVAADAVLVPLQCEFYALEGISGLVRTIDMVKRGLNPDLRLQGIVLTMFDKRNNLSD
                     LVAADARGFFGQTVYETVIPRNIRISEAPSHGKPVLLYDFRSPGAQAYVRLAGEVLRR
                     ERPAAGEAA"
     gene            510..1385
                     /locus_tag="VKQ27_12495"
     CDS             510..1385
                     /locus_tag="VKQ27_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLY89792.1"
                     /translation="MAKRDASPRLGRGLAALLGDAAVQAKPQGGPAVRQIPLDLLEPN
                     PFQPRTVMDQGALAELTQSVRAHGILQPLLIRPHPDATERFQIVAGERRWRAAGSAGL
                     HEVPALVQAMSDSEAAAIALIENLQRQDLNAIDEAEGYDRLLTQFGLTQEALGEAVGK
                     SRSHIANTLRLLNLPASVKEAVRKGAISAGHARALLALPAPDAALREVIGKQLSVRQT
                     EALANRPSRPPPQPGDDTSRGPSADAKALEAMLIQQLGLTTRVAFDGQGGSVQFFYTD
                     LEQLDELLRRLGVQQ"
     gene            complement(1392..2417)
                     /gene="holA"
                     /locus_tag="VKQ27_12500"
     CDS             complement(1392..2417)
                     /gene="holA"
                     /locus_tag="VKQ27_12500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HLY89793.1"
                     /translation="MKVPPQRVAAFLRDPGTTRAVLLYGEDHGMIRDHAATLVRAVAG
                     SVDDPFLVADLGKDQIPQLTSEAASLPLTGGRRVVRLREATDAATDPVASILKGSAPA
                     LVVLEGPGLATRSRLRSLLEAAPDGAAIACYPEEGRALSETIRAALSEGGVTIDADAQ
                     SWLTDQLGADRVSTRQEVDKLALYVGPGGRVDLDAAIACVGDLAGLSLDDALFAATAG
                     DVVLSDRALELAVAEGSTPVGTIRAALTHLQRLHRLRLSVDEGLGVADAVKGARPPIF
                     FRRVGAFTRALGLWSSNALVAAMAGLAEAERACKRTGAPDEVLARNAVLTLARRAAGV
                     AGRHRSQ"
     gene            complement(2424..2951)
                     /gene="lptE"
                     /locus_tag="VKQ27_12505"
     CDS             complement(2424..2951)
                     /gene="lptE"
                     /locus_tag="VKQ27_12505"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HLY89794.1"
                     /translation="MRRLGRRQVVALGLALSGCGFQPVYMPTATGKAGVAQRELAAIH
                     VNLIPDRPGQELRQALQDRLELAGGDVKRRYDLQVGFSVAGEGIAVQNDDTATRVRLI
                     GTAIWTLTAQDSGKTKLSSGFAKAVDAMNILDSQYFALDLENEAVQKRIAETLADQIT
                     MQLAAFFRKRAAAAG"
     gene            complement(2951..5536)
                     /gene="leuS"
                     /locus_tag="VKQ27_12510"
     CDS             complement(2951..5536)
                     /gene="leuS"
                     /locus_tag="VKQ27_12510"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLY89795.1"
                     /translation="MNESVTVAAEAPHYDFRAAEPRWQRAWAERGCFRVDDVPTDNKP
                     KYYVMEMFPYPSGKIHMGHVRNYTLGDVVARYKRARGFSVLHPMGWDAFGLPAENAAR
                     ERNIHPAKWTWENIANMRAELQRMGLSLEWEREFATCDPAYYGHQQKLFLDFLRAGLV
                     ERKQSWVNWDPVDGTVLANEQVIDGRGWRSGALVEKKQLSQWFLKITDYAQELLDALD
                     TLDRWPERVRLMQAKWIGRSEGARLTFLLVQPEACFEGGEVYTTRPDTLFGMSFLALA
                     PEHPLSAAVAARDPKAAEFIAECRRLGTSEAAIETAEKRGYDTGLRVKHPFMPNATFP
                     VWIANFVLMEYGTGAIFGCPAHDQRDLDFARKYGLSVVPVVLPPGKDPATFAVGNEAY
                     VGPGSLINSEFLNGLDVDAAKRAAIEALDRLGFGSGEVNWRLRDWGVSRQRYWGCPIP
                     IIHCQACGVVPVPDDQLPVKLPDDVTFDRPGNPLDHHPTWKHVNCPSCGKQAQRETDT
                     FDTFVDSSWYFARYCSPHADVPVVRPAADHWMPVDQYIGGIEHAILHLLYSRFFARAM
                     KQTGHLNVDEPFAGLFTQGMVTHESYRSAPGPNGAWLYPEEVEKLADGAAIHRTTGEP
                     VVVGRVEAMSKSKRNTVDPEAIITRYGADTARWFILSDNPPERDMEWTEAGVAGAYRF
                     TQRVFRLVENLAPGAAGDGALALLRMTHRTIAAVSEALESFAFNVAVARLYEFANAIA
                     DAERAGVERRDAVEALARLLSPMMPHLAEEMYHRLHPEGVGLVAELAWPEAAPDMLVA
                     DSVTIAVQVMGKLRATIVCPPNAGEAANMAAAMREPNVQRALEGKQIRKTIYVPDRII
                     NFVAN"
     gene            5601..5819
                     /locus_tag="VKQ27_12515"
     CDS             5601..5819
                     /locus_tag="VKQ27_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89796.1"
                     /translation="MMNEFTAVYRPGIFDVANLDEAAPIILTANGASTEHRWATETPY
                     FTDPITRTVAPATHGSRPGERSGGGKAS"
     gene            complement(5816..6379)
                     /locus_tag="VKQ27_12520"
     CDS             complement(5816..6379)
                     /locus_tag="VKQ27_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HLY89797.1"
                     /translation="MTAILKSALIIVVGSMLLAGCGGSVKPNPTDPGYVGQQGPASSG
                     SMPGGSILGDDSVVFGTAKQRPNDSGGAGGTGGGALGVNAYLWRGALDTLSFMPLASA
                     DPFGGVIITDWYTPPTSSNERFKATAYILGRELRSDGVRVSIFRQVLQDGHWVDSSVS
                     PVTVGEIENKVLARARELREQSMANSS"
     gene            complement(6521..>7016)
                     /locus_tag="VKQ27_12525"
     CDS             complement(6521..>7016)
                     /locus_tag="VKQ27_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89798.1"
                     /translation="YTGPGVNTAAGLANLGAAGVRAVGGAFTGQYDGLSLGSGGLALT
                     YAGFTVGGNVIGGRVNGQLGLVPKGGAHETAELVGAKYVTGPWTVGIVAERGDYQGNP
                     LLAGLTQRRGRAIDGGVQYQVAPGMAVWAEYMYQDVWQGGAGGQGTVNVHSQGFLLGD
                     VVNF"
BASE COUNT         1224 a         2390 c         2221 g         1181 t
ORIGIN      
        1 gaaatcgaac tggtcgggct ggacaatcgc gagttccggc ttcgcgaagc tctcgcgccg
       61 ctgcgagccg cgccccggtt ctcctttgtc ctgatcgact gccctcccag cctggggttg
      121 ctcacgctga acggactggt cgctgccgat gcggtactgg tgccgctaca atgtgagttc
      181 tatgcactgg agggcatcag cgggctggtc cggaccatcg atatggtgaa acgtggcctg
      241 aaccccgatc tgcgtctgca gggaatcgtg ctgacgatgt ttgataaacg caacaacttg
      301 tccgatctgg tggcagcgga cgcgcgtggc ttcttcgggc agaccgtcta cgagaccgtc
      361 attccccgca acatccgcat ctccgaggcg cccagccacg gcaaacccgt gctgttgtac
      421 gactttcgtt ctcctggggc gcaggcctat gtgcgcctcg caggcgaagt cctgcgacgc
      481 gagcggcctg ctgcgggaga agccgcataa tggcgaagcg ggacgcgagc ccacgcctgg
      541 gccgaggcct tgccgccctg cttggcgatg cggcggtaca ggcaaagccc cagggagggc
      601 ctgcagtgcg gcagatcccc ctcgatctgt tggagcccaa tccgttccag ccgcgcactg
      661 tgatggatca gggggctctg gcagagctga cccagtcggt gcgtgcccac ggcatcctgc
      721 agccattgct gatccgcccg catcccgacg cgacggagcg cttccagatt gtcgccgggg
      781 aacgtcgctg gcgcgcagcc ggctcagcgg ggctgcacga ggtccccgca ctggtgcagg
      841 ccatgtccga cagcgaagcc gcggcgatcg ccttgatcga aaacctgcaa cgccaggacc
      901 tcaacgcaat cgacgaagcg gaagggtacg accgcctcct cacgcagttc ggcctgaccc
      961 aagaggcgct gggagaggca gtcggcaagt cccgcagcca tatcgcgaac acgctccgcc
     1021 tgctcaatct cccagcatcg gtgaaagaag cggtccgcaa gggcgcgatc tccgccggtc
     1081 atgcacgtgc cttgcttgcg ctcccggcac cagatgccgc cttacgtgag gtgatcggga
     1141 agcagttgtc ggtccgccag accgaggcgc tggccaatcg gccatctcgc ccaccaccgc
     1201 agccaggtga cgacacttca cgcgggccaa gtgccgacgc aaaggcgctg gaagcgatgc
     1261 tcattcagca gcttggcctg accacgcggg tggcattcga cggccagggg ggcagtgtgc
     1321 aatttttcta tactgatctc gagcaactag acgaactgct gcgccgattg ggggttcagc
     1381 agtaggccgc gtcactggga cctatgccga cccgccaccc cagccgcccg acgcgccaag
     1441 gtcagcacgg cgttgcgcgc cagcacctcg tccggggcgc ccgtgcgctt gcacgcacgt
     1501 tccgcctcgg ccaaccccgc catggccgcc accagcgcat tggaggacca taacccaagc
     1561 gctctggtga aggcgccgac ccgccggaag aaaatcggtg gacgcgcgcc cttcaccgca
     1621 tccgcgacac ccaacccttc atcgacggag aggcgcaatc gatgcagccg ctgcaagtgc
     1681 gtcagcgcgg cccggattgt ccccaccggc gtggagccct ccgccaccgc caattcgagc
     1741 gcgcgatcgg acagcaccac atcccccgcg gtcgcggcga acagcgcgtc gtccagcgaa
     1801 agcccggcaa gatcgcccac gcaggcgatc gccgcatcga gatccacccg gccgccgggc
     1861 ccaacataaa gcgccagctt atccacttcc tgccgcgtcg agacccgatc ggcgcccagc
     1921 tgatcggtga gccagctctg cgcatcggca tcgatggtca cgccgccctc actcagcgcc
     1981 gcgcggatgg tttcggacag tgctcgacct tcttccggat agcaggcaat ggctgccccg
     2041 tccggtgctg cctccaacag gctgcgcagg cgcgaccgcg tcgccaggcc cggcccttcc
     2101 aggaccacga gcgccggggc actgcccttc aggatcgatg caacgggatc cgtcgcggca
     2161 tcggtcgcct cacgcagccg gacgacgcga cggccacccg tcagcggcag cgacgccgct
     2221 tcactcgtaa gctgcggaat ctggtccttt cccaggtccg cgaccaggaa gggatcgtcg
     2281 accgatccgg ccaccgcccg cacgagcgtc gcggcgtggt cgcggatcat tccgtggtcc
     2341 tcgccgtaca agagcacggc ccgcgtggtg cccgggtccc ggaggaaggc ggccactcgc
     2401 tggggcggaa ccttcacggc tgatcagccg gcggcagcgg cgcgtttgcg gaaaaatgcc
     2461 gcgagctgca tggtgatctg gtcggccagc gtctcggcga tccgcttctg caccgcctcg
     2521 ttctccagat cgagtgcgaa atactggcta tccaggatgt tcatcgcgtc cacggctttg
     2581 gcgaagccac tgctcagctt ggtcttgcca gagtcctggg cggtcaaagt ccagatcgca
     2641 gtgccgatga ggcgaacgcg ggtggcggta tcgtcgttct ggacggcgat cccttcaccc
     2701 gcgacggaga aaccaacctg gaggtcataa cgccgcttaa cgtcgccgcc ggccagctcc
     2761 agccggtcct gcaatgcctg gcgaagctcc tggccgggcc ggtcggggat cagattgacg
     2821 tggatggccg ccagttcccg ttgcgccacg ccggctttgc cggtggccgt cggcatataa
     2881 accggctgaa aaccgcagcc ggatagggcc aatcccagcg ccacgacctg ccgccgtccg
     2941 agccgtcgca tcagtttgcc acgaagttga tgatgcggtc cgggacgtag atcgtcttgc
     3001 ggatctgctt cccctcgagc gcgcgctgca cgttgggctc cctcatggcc gctgccatat
     3061 tcgcagcctc gccggcgttc ggcgggcaca cgatcgtagc gcggagctta cccatcacct
     3121 gcacggcgat ggtgacgctg tcggccacca gcatgtcggg ggcggcctcc ggccaagcca
     3181 gttcggctac caaccccacg ccttccggat gcagccggtg gtacatttcc tccgccaggt
     3241 gcggcatcat cggcgatagg agccgggcca gcgcttccac cgcatcacgc cgctccaccc
     3301 cagcacgctc ggcgtccgcg atcgcattgg cgaattcgta gagccgagca acggcgacgt
     3361 tgaaggcgaa gctttccaag gcctcggata ctgctgcgat cgtccggtgg gtcatgcgca
     3421 ggagtgcaag cgcgccatcg cccgcagcgc cgggagccag gttctccacc aggcggaaca
     3481 cccgctgggt gaagcgatag gcgccggcga cgccggcctc ggtccattcc atgtcccgct
     3541 ctggcggatt gtccgaaagg atgaaccagc gtgccgtgtc cgcgccatag cgggtgatga
     3601 tcgcctcggg gtccaccgtg ttgcgcttgg acttgctcat cgcttcgacg cggccaacca
     3661 cgactggctc gccggtcgtc cgatggatgg ctgcaccgtc cgcaagcttc tccacttcct
     3721 cagggtagag ccacgcgcca ttgggccccg gtgcgctccg gtagctttcg tgcgttacca
     3781 tgccctgggt gaacaggccg gcgaacggtt cgtcgacgtt gaggtgcccc gtctgcttca
     3841 tggcgcgggc aaagaagcgc gaatagagca ggtgcaggat ggcgtgctcg atgccgccga
     3901 tatactggtc gacgggcatc caatgatcag ccgcggggcg gaccaccggg acatccgcat
     3961 gcgggctgca atagcgggcg aaataccaag aactgtcgac aaaggtgtcg aaggtgtcgg
     4021 tttcccgctg cgcctgcttg ccacaactcg ggcagttgac gtgcttccag gtggggtggt
     4081 ggtcgagcgg attgccggga cggtcgaacg tgacgtcgtc gggtagtttc accggcaatt
     4141 ggtcatctgg gaccgggacg acgccgcagg cctggcagtg gatgatcggg atagggcaac
     4201 cccagtaacg ctgccggctg acgccccagt cgcgcaggcg ccagttcact tcgccacttc
     4261 cgaagccaag ccgatccaac gcctcgatcg ccgcgcgctt ggcggcatcc acatcgagcc
     4321 cattcaggaa ttccgagttg atcagcgagc cggggccgac ataggcctca ttgccgacgg
     4381 cgaaggtcgc aggatcctta cccggcggga gcacgaccgg caccacgctc aatccgtact
     4441 tccgggcgaa atccagatcc cgctggtcgt gcgccgggca gccgaagatc gcgccggtgc
     4501 cgtattccat caacacgaag ttggcgatcc acaccggaaa ggtcgcgttc ggcatgaacg
     4561 ggtgcttcac ccgtaggccg gtatcgtaac cgcgcttttc cgcggtctcg atcgccgcct
     4621 ccgaggtccc cagccgccgg cattcggcaa tgaactcggc ggctttcggg tcgcgcgccg
     4681 cgacagcggc ggacagcgga tgctccggcg ccagcgccag gaaggacatg ccgaacaacg
     4741 tgtcggggcg cgtagtgtag acctcgccgc cctcgaagca tgcctccggc tgaaccagca
     4801 ggaaagtcag tcgcgcgcct tcgctgcgac cgatccattt tgcctgcatc agccggaccc
     4861 gctcgggcca gcgatccaac gtatcgagcg cgtccagcag ctcctgggcg tagtcggtga
     4921 tcttcaggaa ccactggctg agctgcttct tttccaccag cgcgccagaa cgccagccgc
     4981 ggccgtcgat cacctgctcg ttggccagca ccgtgccatc gaccggatcc cagttcaccc
     5041 agctctgctt gcgctcgacg agaccggcac gcaggaaatc gaggaacagc ttctgctgat
     5101 ggccgtaata agccgggtcg caggtggcga actcgcgttc ccattccagc gagagcccca
     5161 tgcgctgcaa ctcggcgcgc atgttggcga tgttctccca ggtccacttc gccggatgga
     5221 tgttgcgctc gcgcgccgcg ttctcggctg gcaggccgaa tgcgtcccag cccatcggat
     5281 gcagcaccga aaagccgcgt gcccgcttgt agcgcgccac gacgtcgccg agcgtgtagt
     5341 tgcgcacgtg ccccatgtgg atcttcccgc tcggatacgg gaacatctcc atgacgtagt
     5401 atttcggctt gttgtccgtc ggcacatcgt caacgcggaa gcagccgcgc tcggcccagg
     5461 cgcgctgcca acgcggttcg gcggcccgga agtcgtagtg cggcgcttcg gccgcgaccg
     5521 tcacgctttc attcatgtcg gatgtatcct gcgccgcacg gcaccgtgca agagccgcgg
     5581 caaacaggga tattgtgcgg atgatgaacg agttcaccgc cgtgtacagg ccgggcatct
     5641 ttgatgtcgc caacctggat gaggcggcgc ctatcatcct caccgccaat ggcgcaagca
     5701 cggagcatcg ttgggcgacc gagacgcctt acttcaccga tcccattacc cgaacggttg
     5761 cgcctgcgac gcacggctcg cggccggggg aacgttccgg tggaggtaag gcttcctagc
     5821 tgctgttcgc catcgactgc tcacgcagtt cgcgggcgcg cgcgagcacc ttgttctcaa
     5881 tctcaccgac agtcaccggg ctgaccgagg aatccaccca atggccgtcc tgcaagacct
     5941 ggcggaagat gctgacccgc acgccatcgc tgcgcaattc gcggccaagg atataggcgg
     6001 tggccttgaa gcgctcgttg ctggaggtgg ggggcgtgta ccagtcggtg ataataacgc
     6061 cgccaaacgg atcggccgag gccaggggca tgaagctgag cgtatccagc gccccccgcc
     6121 acagataggc gtttacgccg agcgcgccac cgccggtgcc gccagcccca cccgagtcat
     6181 tcgggcgctg cttggcggtg ccgaagacca cactgtcgtc gcccaagatg ctgccgccag
     6241 gcatcgagcc gctgctcgct ggaccttgtt ggccaacgta gccagggtca gtggggttgg
     6301 gcttcacact accgccgcag ccggcaagaa gcatcgagcc gacgacgatt atgagggcgg
     6361 atttgagtat tgcggtcatc ccaggttgct catggacaag tagcggcact gcttagcgcc
     6421 tttgtacgcg tcgcgccaag catggccaag ggtttccgtc gaaaaagcaa ggggcggcga
     6481 ggtttccctc gccgcccctg ctgtgaagcc ttggctagtg ttagaagttg accacgtcgc
     6541 caagcaagaa gccctgtgag tggacgttga ccgtcccttg gccaccagca ccgccctgcc
     6601 agacatcctg atacatatac tcggcccaca cggccatgcc cggtgccacc tggtattgca
     6661 cgccaccgtc gatggcgcgg ccacggcgct gggtcaggcc ggccaggaga ggattgccct
     6721 ggtagtcgcc acgttcggcg acgatgccga ccgtccatgg accagtgaca tacttcgcac
     6781 cgaccaactc cgccgtttca tgtgcgccgc ccttcggcac caggcccaac tggccgttca
     6841 cacggccgcc gatcacgttg ccgccgacgg tgaacccggc gtaggtcagc gcgagaccgc
     6901 cgctgccgag gctcaggccg tcatactggc cggtgaatgc gccacctacc gcgcgaacac
     6961 ccgccgcacc gaggttggcc aagcccgccg ccgtgttcac acccgggccg gtgtag
//
LOCUS       DATGEM010000394         6654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_696716, whole genome shotgun sequence.
ACCESSION   DATGEM010000394 DATGEM010000000
VERSION     DATGEM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696716"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VKQ27_12530"
     CDS             complement(<1..270)
                     /locus_tag="VKQ27_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89799.1"
                     /translation="MSLLSVCNLTAAFGTARGEIRAIEDVSFSLDEGEILGIVGESGS
                     GKSVTALTIMGLLPQPPARVAGGSILFAGEELTKLSDTRMQRIRGP"
     gene            complement(422..1330)
                     /locus_tag="VKQ27_12535"
     CDS             complement(422..1330)
                     /locus_tag="VKQ27_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89800.1"
                     /translation="MAPDSAALTLAEALEDAVPVRHHHPAWTTLRRLLHHKLFVTGFA
                     VFAVFVLVALLAPWIAPVDPNKLAMRYRFLPPSAEHLFGTDNFGRSLWSRVIWGAQLS
                     LIIGVSVVAINAVFGTLIGAVAGYFRSFDNVLMRFNDALMAFPAVLLAIGVTSVLGPS
                     VNDVIIALGIVYTPRTARIVRSSVIVLREMEYVQAAVAAGAGHWRILRKHILPNAMAP
                     LIVQLSFLFAYAVLTEATLSFLGVGAVPPTPTWGNIMAEGRQYMTDAPWIIAIPGAAL
                     MVTVLGLNLLGDGLRDVLDPRLRVQQ"
     gene            complement(1320..2270)
                     /locus_tag="VKQ27_12540"
     CDS             complement(1320..2270)
                     /locus_tag="VKQ27_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89801.1"
                     /translation="MLGVILRRLGSALPILVIVSLITFGMIHMIPGDPAAAIAGLSAT
                     PEQIASIRRDLGLDQPLLTQLWRWYVGLLHGDLGRSLLLGQPVLYATMLRLPVTLALS
                     AYALVITLVLGLVSGVIAALRQNTWIDQAAMVFAMIGISVPNFYLGLLMIIFFAVDLG
                     WLPTGGYMPFSTSPLGWLATSTMPAISLALLLAGLLARITRSTMLEVLRQDYIRTARA
                     KGLPRRLVVVKHALSNALIPITTVIGIIVSLLISGSVVTETLFSIPGIGQLLTQAVLN
                     RDYPMVQGGLLITTALLVLVNIGVDVCYALLDPRVRYDGT"
     gene            complement(2274..3842)
                     /locus_tag="VKQ27_12545"
     CDS             complement(2274..3842)
                     /locus_tag="VKQ27_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY89802.1"
                     /translation="MKHTAILSALTVAAALQAQPVWAAPFKCPHVGGDFTFGQEANIN
                     SLDQMTSSTISTRNIAMNMFEALMTRDENFNPINDLAASVTESPDHMTYTFKLRQGVH
                     FHNGKLMTSADVVASFDRYAKVGLERSMLANVDRWDAPDPETFVIHMKKLQPTFLIAL
                     SSFSVPIVIIPAEEKDDPPQQLKTIGTGPFQLIESVPGSYVKIKRYPGYVPNTQFEQR
                     TGFGGYKQACFDTVTFRIVTEPGARVAGLKTGELQGVEDIPAKSLPDIKNDKNITILP
                     LKNWWIQISYPNTSVPPTDNLNFRKAVQAALDMDDIMDAASDGNYSLNVGFQYPNQSD
                     YTDAGKATYNIKDPALAKKYLAESGYKGEPVVLLTDKDYAPMYNSALVMQQQLQAIGI
                     NAQLKVVDWPTSVQMALKGDTGWNYFYTGWGTQPALGALSTMNFFVEPNATYRPKGEV
                     DDPEVLKDWQDMNNLPTPEGRQQAFAAMQKYVLEQAYSLPFGSLTKVQGVRSNVEGFV
                     PFRIPRVFNVWFTH"
     gene            complement(4042..4626)
                     /locus_tag="VKQ27_12550"
     CDS             complement(4042..4626)
                     /locus_tag="VKQ27_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HLY89803.1"
                     /translation="MRDTVVVFARAPRLGAVKRRLARDIGDRAALRFHLATMTRLVRA
                     LAGVRRFRVIVAMTPDRARVRLPGARVAQGGGDLGVRMHRAFWHWPRGRVAIIGCDIP
                     EAGVADVLAAFRALGRADAVFGPAEDGGYWLVGMGPRRPAQPFAGVRWSSEDALADTL
                     ANFRGRRIVMLRMLHDVDSGEDWRQYNLNTDQAD"
     gene            complement(4623..5267)
                     /locus_tag="VKQ27_12555"
     CDS             complement(4623..5267)
                     /locus_tag="VKQ27_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HLY89804.1"
                     /translation="MIPALNAAATLGATLKSVEGADEVVVVDGGSEDGTVAVAERLGA
                     RVVAAPRGRGGQLAAGVEAARGEWLLLLHADTRLEEGWLRVSPQGGRRGEEQAYYFRL
                     VLDCDDPRARRLERLVAWRSRVLGLPYGDQGLLIHRDLLRSVGGVRVLPLMEDVDLVR
                     RLGRRRLAAVDVAAVTSAAKWVRDGWYRRSARNLVCLGLYFLGVPPRVIARLYG"
     gene            5351..6469
                     /locus_tag="VKQ27_12560"
     CDS             5351..6469
                     /locus_tag="VKQ27_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HLY89805.1"
                     /translation="MRDDSEAGNMPSLARHGRGATTNPPVRFESQAIAPFDDGWDTLA
                     TEFGELPKLATTLTKDSTRSAISWNSSPDIGFDRAVNPYRGCEHGCIYCYARPTHAYL
                     GYSPGLDFETKLIFKPEVADILEKEFRKPGYTARTMALGSNTDPYQPVERTLKLTRAI
                     LQVLDRYNHPVGIVTKSAGVLRDLDILSAMARRKLARVYLSVTTLDPALARIMEPRAA
                     TPARRLHAISELTRAGVPTGVMVAPMIPGLNDAEMEKIVEAAARAGARHAGYVLLRLP
                     HELKQMFEDWLATHFPDRARHVLSLIRETRAGGLSDARFHHRFAGQGVYADLLLRRFA
                     RATRQWGLDEAREGLDCGKFAVPDDGRPKVTEAQMSLF"
BASE COUNT         1316 a         2571 c         1738 g         1029 t
ORIGIN      
        1 cggcccccgg atgcgctgca tccgcgtatc cgacagcttc gtcagctctt cgccggcaaa
       61 caggatactg ccgcccgcca cccgagccgg cggctgtggc agcagtccca tgatggtgag
      121 cgcggtgaca ctcttccctg acccggactc cccgacgatc cccaggatct cgccctcatc
      181 cagcgagaac gacacatcct cgatcgccct gatctccccg cgcgcggtgc caaacgccgc
      241 cgtcagattg cagacagaaa gcaggctcat aacgtaccag aaaagaagga ttcacacgga
      301 gaccacgaag gttccacgga gaaccacgga tcaaaatgaa tgcgcgcgca gcgcgcgccc
      361 agaatgctta cctcagtggc cctccgtggt tctcggtgtt ctccgtgtgg aaccttcctt
      421 ttcactgctg caccctcaac cgcggatcca gcacgtcccg caacccatcc cccagcaggt
      481 tcaagcccag cacagtgacc atcagcgccg cccccggtat cgcaatgatc caaggcgcgt
      541 cggtcatgta ctgccgcccc tccgccatga tgttccccca tgtcggcgtc ggcggcaccg
      601 cacccacccc caggaacgaa agcgtcgcct cggtcagcac cgcgtaggcg aacagaaagc
      661 tcaactgcac gatcagcggc gccatcgcgt tcggcaggat gtgcttccgc aggatccgcc
      721 agtgccccgc ccccgccgcg acagccgcct gcacatactc catctcgcgc agcacgatca
      781 ccgacgaccg cacgatccgc gccgtccgcg gcgtatacac aatccccagc gcgatgatca
      841 cgtcgttcac cgacggccca agcaccgacg tgaccccgat cgccagcagc accgccggaa
      901 acgccatcag agcgtcgttg aaccgcatca gcacgttgtc gaacgaccgg aaataccccg
      961 ccaccgcccc gatcaaggtg ccgaacaccg cgttgatcgc caccaccgac accccgatga
     1021 tcagcgacag ttgcgcgccc cagatcaccc gagaccacag cgagcgcccg aaattgtcgg
     1081 tgccgaacag atgctccgcc gagggaggca agaaccggta ccgcatcgcc agcttgttcg
     1141 gatccaccgg cgcgatccac ggcgccagca acgccaccag cacgaacacc gcgaacaccg
     1201 cgaacccggt gacgaacagc ttgtgatgga gcaatcggcg cagcgtggtc caggccggat
     1261 ggtgatgccg caccggcacc gcatcctcca gcgcctctgc cagcgtcagt gccgcgctgt
     1321 caggtgccat cgtagcgcac ccggggatcg agcagcgcgt agcacacatc gaccccgata
     1381 ttcaccagca ccagcagcgc cgtggtgatc agcagcccac cctgcaccat cggatagtcg
     1441 cggttcagca ccgcctgcgt cagcaactgc ccgatccccg gaatcgagaa cagcgtctcc
     1501 gtcaccaccg acccggaaat cagcaagctg acaatgatcc cgatcaccgt ggtgatcggg
     1561 atcagcgcgt tggacagcgc atgcttcacc accaccaggc gccgcggcag ccctttcgcg
     1621 cgcgccgtcc ggatataatc ctgccgcagc acctccagca tcgtggaccg cgtgatccgc
     1681 gccagcagcc ccgccagcag cagcgccagc gatatcgccg gcatggtgga tgtcgccagc
     1741 caccccagcg gactcgtgct gaacggcata tatccgcccg tcggcagcca ccccaggtcc
     1801 accgcaaaga agatgatcat cagcagcccg agatagaaat tcggcaccga gatcccgatc
     1861 atcgcgaaca ccatcgccgc ctggtcgatc caggtgttct gccgcaacgc cgcgatcaca
     1921 ccgctgacca ggcccagcac cagcgtaatg acaagcgcat aggcggaaag cgccagcgtc
     1981 accggcagcc gcagcatcgt cgcatacagc accggctgcc ccagcagcag cgaccgcccc
     2041 agatcgccgt gcagcagccc cacgtaccaa cgccagagct gcgtcagcaa gggctgatcc
     2101 agccccaggt cacgccggat cgaggcgatc tgctccggcg tggcactcag cccggcgatc
     2161 gctgccgccg gatcgcccgg gatcatatgg atcatgccga acgtgatcag gctgacgatg
     2221 accagtatcg gcagcgcgct tcccaggcgc cgcagaatga cgcccagcat ccctcagtgc
     2281 gtgaaccaca cattgaacac gcgaggaatc cggaacggca caaacccctc gacgttcgac
     2341 cgtacgccct gcaccttggt cagcgacccg aaaggcaaag aatacgcctg ctccagcacg
     2401 tatttctgca tggcggcaaa agcctgctgc cgcccctccg gcgtcggcag gttgttcatg
     2461 tcctgccagt ccttcagcac ctcgggatcg tccacttcac ccttcggccg ataggtcgca
     2521 ttcggctcga cgaagaagtt catggtagag agcgcgccca gcgccggctg cgtcccccat
     2581 cccgtataga agtagttcca cccggtatcg cccttcagcg ccatctgcac cgaggtcggc
     2641 cagtccacca ccttgagctg cgcattgatg ccgatggcct gcaactgctg ctgcatcacc
     2701 agcgccgaat tgtacatcgg cgcatagtcc ttgtcggtca gcagcaccac cggttcgccc
     2761 ttgtagcctg attccgccag atacttcttc gccagcgccg gatccttgat gttgtaggtc
     2821 gctttgccgg catcggtgta atcggactgg ttcggatact ggaacccgac gttcagcgag
     2881 tagttgccat ccgatgcggc atccatgatg tcgtccatat ccagcgccgc ctgcaccgcc
     2941 ttgcggaaat tcagattgtc cgtcggcggc accgaagtgt tgggatacga aatctggatc
     3001 caccagttct tcagcggcag gatcgtgatg ttcttgtcgt tcttgatgtc cggcagcgac
     3061 ttggcaggaa tatcctccac gccctgcagc tcgcccgtct tcagccccgc aacgcgcgcg
     3121 ccaggctccg tcacgatgcg gaacgtgacc gtatcgaaac acgcctgctt gtagccgccg
     3181 aacccggtcc gctgctcgaa ctgcgtgttc ggcacatacc ccgggtaccg cttgatcttc
     3241 acgtagctgc ccggcacaga ctcgatcagc tggaacggcc ccgttccgat cgtcttcagc
     3301 tgctgcggcg gatcgtcctt ctcctccgct ggaatgatca cgatcggcac gctgaacgaa
     3361 ctcagcgcga tcaggaacgt cggctgcaac ttcttcatgt gaatgacaaa ggtctcggga
     3421 tccggcgcat cccagcgatc gacattggcc agcatggagc gttcgagccc cacctttgca
     3481 tagcgatcga acgaggccac cacatcggcc gatgtcatca gcttgccgtt atggaaatgc
     3541 acgccctgcc gcagcttgaa cgtgtatgtc atgtggtccg gcgactctgt caccgaggcc
     3601 gcaagatcat tgatcggatt gaagttctca tcacgcgtca tcaacgcctc gaacatgttc
     3661 atggcgatgt tgcgcgtgga aatagtggaa gaagtcatct ggtcgagcga attgatgttc
     3721 gcttcctgac caaaggtgaa atcgccgccg acatgcgggc atttgaacgg cgctgcccat
     3781 accggctgtg cctgaagtgc cgcagcgact gtcagcgcgc tgaggatggc ggtgtgcttc
     3841 atcgttatcg tcccccgatc acccgcccta gaccgcggac tgaccaccgc ttgggcacgc
     3901 aacgtgacga gacggggatg tgccgtcaag gccggcgcct gcgagtcaga aacaaagaat
     3961 tacaacacgg attacgcgga ttccgtcacg gatttcacgg aaagaaaata ttataaaata
     4021 ctcaaccgtg caatccgtgt atcagtccgc ctgatccgtg ttcaaattat actgtcgcca
     4081 atcctcacca ctgtccacgt catgcagcat ccgcagcatg acaatccgcc gcccacgaaa
     4141 gttcgccaac gtatccgcca acgcatcctc gctcgaccac cgcaccccag caaatggctg
     4201 cgccggccgc cgcggcccca tccccaccag ccaatacccc ccatcctccg ccggcccgaa
     4261 cacggcatcc gcacgcccca gcgcccgaaa cgccgccagc acatcggcca ccccggcctc
     4321 cggaatatcg catccgatga tcgcgacgcg cccccgcggc cagtgccaga aagcccgatg
     4381 catccgcacg ccaagatcgc cgccgccctg cgccacacgc gcaccaggca gccgcacccg
     4441 tgcccgatcc ggcgtcatcg ccacgatcac ccgaaaccgt cgcacgcccg ccagcgcccg
     4501 caccagtcgc gtcatcgtcg ccaaatggaa ccgcagcgcc gcccgatcgc cgatatcccg
     4561 cgccagccgc cgcttcaccg cccccagccg aggcgcccgc gcgaacacca cgaccgtatc
     4621 cctcatccgt acagccgggc gatcacccga ggcggcacac ccaggaagta cagccccagg
     4681 caaaccagat tgcgcgccga ccgccggtac cacccgtccc gcacccactt agccgccgag
     4741 gtcaccgcag ccacatccac cgcggccagc cgtcgccgcc ccaaccgccg caccaggtcg
     4801 acatcctcca tcagcggcaa cacccgcacg ccccccaccg accgcagcag gtcccgatgg
     4861 atcagcagtc cctgatcccc ataaggcaac cccaacaccc ggctccgcca agccaccaac
     4921 cgctccagcc tccgagcccg cggatcatcg caatccagca ccaaccggaa ataatacgct
     4981 tgctcctccc ctctccgccc cccctggggg gaaacccgca accacccctc ctccaaccgg
     5041 gtatccgcat gcagcagcaa cagccactcc cctcgcgccg cctcgacacc agcggccaac
     5101 tgcccgcccc gtccccgagg cgccgccacc acccgcgccc ccaaccgctc ggccaccgcg
     5161 accgtgccgt cctcgctccc cccatccacc acgaccacct cgtcggcccc ctcgaccgac
     5221 ttcagcgtcg cccccagcgt cgccgccgca ttcagcgcag ggatcacaac ggacaacatg
     5281 ttcctacaat gttcttgacc ccgtccattc gctgcgccac tcttctccag gaacggacgg
     5341 agaacacgtc atgcgcgacg actctgaagc tggcaacatg ccatcgctcg cccgccacgg
     5401 ccgtggtgcc accaccaatc cgcccgtccg cttcgaaagc caggccatcg ccccgttcga
     5461 tgacggctgg gacacgctcg ccaccgagtt cggcgaactc cccaagctcg ccaccaccct
     5521 cacgaaggac tccacccgct ccgcgataag ctggaacagc tcgccggaca tcggcttcga
     5581 ccgtgcggtg aacccctacc gaggctgcga gcacggctgc atctactgtt atgcccgtcc
     5641 cacccatgcc tatctcggct actcccccgg cctcgacttc gagaccaagc tgatcttcaa
     5701 gcccgaagtt gccgacatcc tcgagaagga attccgcaag ccgggctaca ccgcccgcac
     5761 catggcgctc ggctccaaca ccgatcccta ccagccggtc gagcgcaccc tgaagctgac
     5821 ccgcgccatt ctccaggtgc tggaccgcta taaccacccc gtcggcatcg tcaccaagtc
     5881 ggccggcgtg ctgcgcgatc tcgacatcct cagcgccatg gccaggcgca agctcgcccg
     5941 cgtctacctc tccgtcacca ccctggaccc ggcgctcgcc cgcatcatgg agccgcgcgc
     6001 cgccactccc gcccgccgcc tgcacgccat ctccgagttg acccgtgccg gcgttccaac
     6061 cggcgtcatg gtcgccccga tgatccccgg ccttaacgac gccgagatgg agaaaatcgt
     6121 cgaagccgca gcccgcgccg gcgcccgcca tgccggctac gtcctgctcc gcctccccca
     6181 cgaactgaag cagatgttcg aggactggct cgccacccac ttcccagacc gcgccaggca
     6241 cgtcctcagc ctgatccgcg agacgcgtgc cggcgggctc agcgacgccc gcttccacca
     6301 ccgcttcgcg ggccaggggg tctatgccga cctcctcctc cgccgcttcg cccgtgccac
     6361 ccgccagtgg ggcctggacg aagcccgcga gggcctcgac tgcggcaaat tcgccgtccc
     6421 cgacgacggc cgccccaaag tcaccgaagc ccagatgtcg ctgttctgac cctgccgaac
     6481 ccgccgtgtt gccaacctgc cgaccgggtg gtaccgcccg gccaggccaa tatgagcgat
     6541 ctcgccatca tcaccggttc cgacgccggc tatttccccc tggtccagga actgatcgcc
     6601 tcggtcgagc acgccgcccc atctggcatc tgcaccttcg gcatgctgga cgcc
//
LOCUS       DATGEM010000395         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_927163, whole genome shotgun sequence.
ACCESSION   DATGEM010000395 DATGEM010000000
VERSION     DATGEM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927163"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VKQ27_12565"
     CDS             complement(<1..527)
                     /locus_tag="VKQ27_12565"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HLY89806.1"
                     /translation="MSFRRGAFRAVLLATLALAPFAARAQSDQQLTVDRAIKTVGDLT
                     HDKAFGNAHQLLGRARAVLIVPKLFKGGFIVGGEGGTGVLLAREPGGRWSDPAFYAIG
                     SASLGFQAGLEQSEMVLLIMTQKGLDAILRDQFKVGAQAGIAVANLGSGVEGAVSAGG
                     PDIVVWSSSSGVYAGV"
     gene            complement(668..1054)
                     /gene="crcB"
                     /locus_tag="VKQ27_12570"
     CDS             complement(668..1054)
                     /gene="crcB"
                     /locus_tag="VKQ27_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HLY89807.1"
                     /translation="MGTVTTWLAVAVGGALGSLARFWIAAVMTMLTGPRFPWGTLLIN
                     VLGSFVIGLVAGLTAMPPRVGMHPDVRVFLMVGVCGGFTTFSAFSLQSLELLQAGEMV
                     PAVGYMFGSVVLCLIGVWAGWLLGRA"
     gene            1257..1697
                     /locus_tag="VKQ27_12575"
     CDS             1257..1697
                     /locus_tag="VKQ27_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY89808.1"
                     /translation="MARLLRVDADKRARAHGMTRAQWGILIWLERQPGLSQKELAEIL
                     EVEPITVARLIDRLEGRGMVERRPDPKDRRIWRLHLCPPAIPVLREIDLQRIDMRDLI
                     TDGLDEITIETMTEALLTMKSILIHDAHPPRRYSPIQTEARETV"
     gene            1698..>2211
                     /locus_tag="VKQ27_12580"
     CDS             1698..>2211
                     /locus_tag="VKQ27_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HLY89809.1"
                     /translation="MSQATATAGPASAARVTEAPVRTRRLSRSLLRPLLMLGGIAVVI
                     VGAGAYWLTGGRYVSIDDAYVRAAKETLSTDVSGIVADVPVHEGQRVKKGDVLLRLDP
                     KQFEIMVQSAKGALGAKTWDLNALKVDYQRMLRDVAVKEAQVQADQANFERYSNLVKS
                     GGVTRADYDNA"
BASE COUNT          426 a          775 c          653 g          357 t
ORIGIN      
        1 acgccggcgt aaaccccgct tgacgagctc cacaccacga tgtccggccc accggcgctc
       61 accgcgccct cgacgccgga tccaaggttc gccacagcga tccccgcctg cgcgccgacc
      121 ttgaactggt cgcgcaggat cgcgtcgagg cccttctgtg tcatgatcag cagcaccatc
      181 tcggactgct ccagccccgc ctggaagcca agcgatgccg aaccgattgc atagaacgcc
      241 ggatcgctcc accggccgcc aggttcgcgc gccagcagaa caccggtgcc gccctcgccg
      301 cccacgatga agccgccctt gaacaatttt ggcacgatca gcactgcgcg cgcgcggccc
      361 agcaactggt gcgcgttgcc gaacgccttg tcgtgcgtca ggtcgccgac cgtcttgatc
      421 gcgcgatcca ccgtaagctg ctggtccgat tgtgcccgcg cggcgaacgg ggcgagcgct
      481 agtgtggcga gaagaacggc gcgaaacgcg cccctgcgga agctcatgtg caacccccaa
      541 tttccgttgc ggggtggcaa atggccgcgc caatcgggcc gctttgtggc ccgtcgggca
      601 tcctgggatc acacttggct gaggaccgca ccactgtttc ggccccgggc gtcctgggtt
      661 gtgaagctca agcccgcccc aatagccacc ccgcccaaac gccgatcaga cacaggacaa
      721 ccgatccgaa catatagccg accgccggca ccatttcgcc cgcctgaagc aactccaggc
      781 tttgcaggct gaatgccgag aacgtggtga acccgccgca gacccccacc atcaggaaca
      841 cccgcacgtc cggatgcata ccgacccgtg gcggcatcgc cgtcagcccc gccaccagcc
      901 cgatgacgaa tgatccgagc acattgatca gcagcgtgcc ccaggggaac cgcggccccg
      961 tcagcatggt catcaccgcc gctatccaga atcgcgcaag gctccccagc gctccgccga
     1021 ccgctacggc gagccacgtc gttaccgtcc ccatggacag ccgctataca agacatgccg
     1081 gtatccgccc aggacggcag gatcaggcaa gacaagcggg tccgccctgc gtgcggcgca
     1141 tggacttacc gctatcctcc ttatcataac gcagcttatt atcactcctt cctaggaaag
     1201 ggaccgtcta gcctgtgcac actttccaac gcgacctgtt gttcttgctt cacgacgtgg
     1261 cccggctgct gcgcgtggat gccgacaaac gggcccgtgc gcatggcatg acccgcgccc
     1321 agtggggcat cctgatctgg ctggagcgcc agcctggcct gtcgcagaaa gagctcgcgg
     1381 aaatcctgga agtagagccg atcacggtcg cccgcctgat cgacaggctg gaggggcgcg
     1441 ggatggtgga gcgtcgtccg gaccccaagg accggcgcat ttggcgcctg catctgtgcc
     1501 cgcctgccat ccccgttctg cgggaaatcg accttcagcg catcgatatg cgggacctga
     1561 tcaccgacgg cctggacgag ataacaatag aaacgatgac ggaagcccta ctgacgatga
     1621 agtccatctt gatccacgac gctcatccgc cgcgccgcta ctcaccaatc caaaccgaag
     1681 cgcgggagac cgtatagatg tcgcaggcca ctgccaccgc cggacccgca tccgccgctc
     1741 gcgttaccga agcgcccgtc cgcacccgca ggctcagccg gtccctgctg cgtccgctgc
     1801 tgatgctggg gggcatcgcc gtcgtcatcg tcggtgccgg cgcctactgg ctgactggcg
     1861 gccgttacgt gtcgatcgat gatgcatatg tgcgcgccgc caaagaaacg ctgtccaccg
     1921 acgtgtccgg catcgtcgcg gatgtcccgg tgcatgaagg ccagcgcgtc aagaagggtg
     1981 acgtgctgct gcgcctcgat cccaagcagt tcgagatcat ggtgcaatcc gccaaggggg
     2041 cgcttggggc caagacatgg gacctcaacg cgctgaaggt cgattaccaa cgcatgctgc
     2101 gcgatgtcgc cgtgaaggaa gcccaagtcc aggccgatca ggcaaatttc gagcgctatt
     2161 cgaacctggt gaagtcaggc ggtgttaccc gcgccgatta cgacaatgcg c
//
LOCUS       DATGEM010000396         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_984105, whole genome shotgun sequence.
ACCESSION   DATGEM010000396 DATGEM010000000
VERSION     DATGEM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984105"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VKQ27_12585"
     CDS             complement(<1..500)
                     /locus_tag="VKQ27_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY89810.1"
                     /translation="MPVNTSLRFGVVGLGRAAAATVPAILRNPNTSVVAAADSNAEVL
                     EAFAQRYAARTYGTIEELCADRDVDAVYIATPTHLHTQHVLTAAAAGKHVICEKPLAI
                     TLEDADRMIAAVDAQRVHFIVGQSQSYEPPVRAIRELVRQGTLGRLALINGWYYNDWL
                     YRPRLPA"
     gene            complement(490..1524)
                     /locus_tag="VKQ27_12590"
     CDS             complement(490..1524)
                     /locus_tag="VKQ27_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89811.1"
                     /translation="MSELRKRGRTGVIVLIVAAAFSVPFWVAGFLTFQLAAAFSYAIA
                     LLGLTLITGRAGLVSIGHSASFGVGAYTGVVLIWLLHLPYGWAIPAGAVTGFIFGALV
                     GLPITRLSKNRQSMVIVTLALAIAMPQILKRFPEYTGGTNGVNLPTPAPPSVLPLAQD
                     QWIYLLSLCLLLLMFWFCRNLLHGRIGRAMTAMRNHEVAAATSGVNVPAYKLLAFAFG
                     SMFAGVGGAVFVLVTQFANPDSFSLVLSITFLTGGIIGGIDSAVGAILGALFVEFVPV
                     VTSDMAMAHDFPPAIIYAIVLVAVIFLMPDGLVGLAARVRRRRSAVNRVDGVDRAAGA
                     VPSMQEELAR"
     gene            complement(1517..2398)
                     /locus_tag="VKQ27_12595"
     CDS             complement(1517..2398)
                     /locus_tag="VKQ27_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY89812.1"
                     /translation="MQSFLQLVASGIATGIIFGSLGLAFVLVFQATRTLNLAQGEIAA
                     LGTFGAWTLLQLGLPYWAVFVIMTGASFVGGFVIHRLLVRPLDGKSHFSVIVVTLALL
                     IGINGFNLFVWGGDINQFPTPFGQRPVNLGGVIVNSHDLGTIAVLALVLVLLYLLIER
                     TNIGLVLRATAQNRISAELIGIPTDLTVGIGWGLASGLGTIAGMLAAPVAFLEPGMMI
                     NLLPYALAAITLGGLTSPVGALIGGILVGVFENLLGNYVGFIGQDLKLPVVLLLMAIV
                     LLFRPQGLFGKVEITRV"
     gene            complement(2398..>2577)
                     /locus_tag="VKQ27_12600"
     CDS             complement(2398..>2577)
                     /locus_tag="VKQ27_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89813.1"
                     /translation="LQRLNASERVTMLLVEQNASIALRLATFANVLETGHMVLSGRAD
                     VLQQNDAVRRAYLGY"
BASE COUNT          568 a          817 c          799 g          393 t
ORIGIN      
        1 gctggaagtc gcggtcgata gagccagtcg ttgtagtacc acccattgat gagcgcaagt
       61 cgaccaagcg tgccctggcg caccagctcg cgaatcgccc gaacaggtgg ctcgtaactc
      121 tgcgattgtc cgacgatgaa gtgcacgcgc tgcgcgtcga cggcggcgat catccgatcc
      181 gcgtcctcca gcgtgatcgc cagcggtttt tcgcagatga cgtgttttcc agcggcagcg
      241 gcggtcagca catgttgggt atgcagatgt gtcggcgtcg cgatgtacac cgcatccacg
      301 tcgcgatcag cgcataattc ctcgatagtc ccataggtcc gcgctgcgta gcgttgcgcg
      361 aatgcctcca gcacctcggc gttgctgtcg gccgcggcca cgaccgacgt gttcgggttt
      421 cggaggatgg ctggtaccgt ggcggcagca gcccggccaa gtccgacaac accgaagcgc
      481 agcgaggtgt taacgggcaa gctcttcctg catgctggga acagcccccg ccgcccgatc
      541 cacgccgtcc acccggttca ccgcggaccg gcggcgacgc acccgcgctg ccagccccac
      601 cagaccatcc ggcatgagaa aaatcaccgc caccagcacg attgcgtaaa tgatcgccgg
      661 cggaaaatca tgcgccatgg ccatgtcgga cgtgacaacc ggcacgaact cgacgaacaa
      721 tgcgccgaga atggcaccaa ccgccgagtc gatgccgccg ataatgccac cggtcagaaa
      781 cgtgatcgac agtacgagcg agaagctgtc tggattggcg aattgcgtca cgagcacaaa
      841 cacggcaccg ccgactcccg cgaacatcga gccgaatgcg aacgccagga gtttgtaggc
      901 cggcacgttg actcccgacg tggcggcggc cacttcgtgg ttgcgcatcg ccgtcatcgc
      961 gcgcccgata cgcccgtgca acaggttgcg gcaaaaccag aacatcagca gcagcagaca
     1021 gagcgacagc aggtagatcc attgatcttg cgcaaggggc agcactgatg gtggcgctgg
     1081 tgtcggcagg ttcacaccgt tggttccacc ggtatattcg gggaagcgct tcaggatctg
     1141 cggcatcgca atcgccagcg cgagcgtgac gataaccatg ctctgtcggt ttttggacaa
     1201 cctcgtgatg ggcagaccga caagtgctcc gaagataaat ccggtgactg cgccggcggg
     1261 aatggcccag ccatacggca ggtgcagcag ccagatgagg accacgccgg tgtacgcgcc
     1321 cacgccgaaa ctggcggagt ggccgatgga gaccaggccg gcgcggccag tgatcagggt
     1381 gaggcccagc agcgcgatgg cgtacgagaa cgccgctgcc agctgaaagg tcagaaaacc
     1441 ggcgacccag aatggcaccg agaaggctgc cgccacaatc aggacgatca cgccagtgcg
     1501 gccgcgtttg cggagctcag acacgcgtga tctcgacctt gccgaataac ccttgtgggc
     1561 ggaacagcag cacaatcgcc atcagcagca gaaccaccgg cagtttcaga tcctgcccga
     1621 tgaaaccgac gtaattgccc aaaagattct cgaacacgcc cacgaggatc ccgccgatca
     1681 gcgcgcctac cggactcgtc aggcctccaa gggtgattgc ggccaacgcg tatggcagca
     1741 ggttgatcat catgcccggt tcgaggaagg caaccggcgc cgccaacatc ccggcgatgg
     1801 tcccaagccc actggccagg ccccaaccga tacccacagt caggtcggtt ggaatgccga
     1861 tcagctcggc cgatattcga ttctgcgccg tcgcccgcaa caccagaccg atgttggtgc
     1921 gctcgatgag caggtacagc aaaaccagca cgagcgccag caccgcgatg gtgcccagat
     1981 cgtgcgagtt cacgatcact ccgccaagat tgactgggcg ctggccaaac ggcgtgggga
     2041 actggttgat gtcgccgccc cagacgaaca ggttgaaccc attgatgcca atgagcagcg
     2101 cgagggtaac gacgatgacg gagaagtgcg acttgccgtc aagtgggcgg accagcagcc
     2161 ggtggatgac gaagccacca acgaacgacg cgccagtcat gatgacgaac accgcccagt
     2221 acggcaggcc caactggagt aaggtccacg cgccgaacgt gccaagcgcg gcgatctcgc
     2281 cctgggccag attgagcgtt cgcgtggcct ggaagaccag aacgaaggcc aggccgaggc
     2341 tgccgaaaat gatgcccgtt gcgatgccgc tggcgaccaa ctggaggaag gactgcatca
     2401 gtaccccagg tacgcgcgac gcacggcgtc gttctgctgc agcacgtccg cgcggcccga
     2461 cagcaccatg tggccagttt ccaggacatt ggcaaatgtc gcaagacgca gcgcgatcga
     2521 ggcgttctgc tcaaccagga gcatcgtcac ccgctccgag gcattcagcc gctggag
//
LOCUS       DATGEM010000397        22670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1637962, whole genome shotgun sequence.
ACCESSION   DATGEM010000397 DATGEM010000000
VERSION     DATGEM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 22670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22670
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637962"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VKQ27_12605"
     CDS             <1..444
                     /locus_tag="VKQ27_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89814.1"
                     /translation="ERGVFGGLTVEENLRIAALTAPAPWGLDRIYAMFSRLAERRRSR
                     GGSLSGGEQQMLTIARALIRDARIILLDEPFEGLAPLIVRDLVRISRELAAQGRTIIV
                     VEQNVVAALSFADRVYILNNGHVVYEGTPDSLHANPEVMKSHLGV"
     gene            complement(452..628)
                     /locus_tag="VKQ27_12610"
     CDS             complement(452..628)
                     /locus_tag="VKQ27_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HLY89815.1"
                     /translation="MGLLLLILIIVLLFGGGGYYGYRSGYYGGAHYGGGFGLILLLLV
                     LFLLFGAPGYWGHY"
     gene            742..1743
                     /gene="epmA"
                     /locus_tag="VKQ27_12615"
     CDS             742..1743
                     /gene="epmA"
                     /locus_tag="VKQ27_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HLY89816.1"
                     /translation="MPQPTWDPDSLAARLPFLHRRALLTAGTRAYFTARGYTEVETPF
                     AVPAPGEEVHLRAFRTEREYPDGASKPLWLHTSPEFAMKKLLVAGAGPIYQLARVWRN
                     GEGSDLHAPEFTMLEWYHPGATMDSLIDETTAYLRAVLPPVVTCRGVTTDLSRIERMT
                     VADAFTRYVGADVLAAADDAPSLAAAAGTRLREGETWEDLFFRLLLERVEPNLGRAHP
                     TFLTHWPAAQAALARRDPADPRVAERFELFVCGIELANAFVELTDADEQRARFESDRA
                     RRHAISGEAWPLDEDFLAALTFGMPPSAGIALGFDRLAMIASGANRIDQVLWLPEPD"
     gene            1776..2123
                     /locus_tag="VKQ27_12620"
     CDS             1776..2123
                     /locus_tag="VKQ27_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89817.1"
                     /translation="MRSAALLLLAGLLGGCANQLAERQAYLKQFIGQPESSVVQAMGV
                     PSRTFQTGGVTYLAYNEHRIDFIPGTPTYAPSYLGSYGAFPPQIVQLDCETTFQVVDG
                     KIASFQLRGNACG"
     gene            complement(2165..2638)
                     /locus_tag="VKQ27_12625"
     CDS             complement(2165..2638)
                     /locus_tag="VKQ27_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HLY89818.1"
                     /translation="MTDAILAAHADTDQPLATFRAIDAALAREPGHILFTVLVHHAAL
                     RQSERFYTNKPEAYPIGGRKPVTDSPWMQRVIHGGNPYIGRTREDIAANFFDHELIHS
                     LGCDSILNMPVRWRGQTMGTLNLCHRAGYYDESHMPRMRLIAQLALPALLLVQNR"
     gene            complement(2685..3650)
                     /locus_tag="VKQ27_12630"
     CDS             complement(2685..3650)
                     /locus_tag="VKQ27_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLY89819.1"
                     /translation="MAQPKSKGTKGKTRPRARSNSGSNGSGTSPARDYSIAAVGRALD
                     LLEALSRIGPAPLAALADAARCTRTAGFRLLRTLEARGFAIQDEARGMWRLGARWGVL
                     GRAAVEQGALAATAMPFLAALGKTSGENVYLRVRDGLESETIAIYQTDPGLRLYSEVG
                     KRQTLHAGSSRLLLAHAPEAVQTQVLAQRLPRFTPATRTDSAWIAADLQRIRTRGYLM
                     TTDEVVAGAATVAAPIRDASGQVVAVLSVSAPTMRMRPPRPRALLPMVLDAAQKLSRM
                     LGAGGGEAAPRAGASERKGASTATRSEGVVLAPALAAPGPHSIFR"
     gene            4032..4116
                     /locus_tag="VKQ27_12635"
     tRNA            4032..4116
                     /locus_tag="VKQ27_12635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4066..4068,aa:Leu,seq:tag)
     gene            4149..5501
                     /gene="tig"
                     /locus_tag="VKQ27_12640"
     CDS             4149..5501
                     /gene="tig"
                     /locus_tag="VKQ27_12640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HLY89820.1"
                     /translation="MQVTETLSEGLKRAYTVVVPAADLETKRAARLANLSKTLTLPGF
                     RPGKIPPNIVKQRFGTAVGAEVLEESVSEATQQVLSERGLRPAQQPKVDVVSLDPTGT
                     TGKDLEFKVELELLPEVTPPDFSAIKLTRLKTEPDGETIDKGLNEIAQRNRTLEEVPA
                     EELGERGAATGEVLTVDFTGKMDGTEFPGGAGKDIEVEIGGSNFIPGFSEQLEGMKPG
                     ETRTIEVTFPADYGVATLAGKPATFDVTAHKISRGNVPAVDDELATKLGFEDLAEMRD
                     TITKRFQSEYDQLSRLRLKRQLLDALADTVKFESPEGMVNQEFDQIWQRLESERQQGR
                     LDDDDKDKDEETLRSDYRAIAERRVRLGLLLAEIGRINSITVQPDEMTRAMRAEAMRF
                     PGQEQQVFEFFRQNPRATDSLRGPLFEEKVINFILELAQVEDQTVSLEELTKEPPSPV
                     "
     gene            5521..6402
                     /locus_tag="VKQ27_12645"
     CDS             5521..6402
                     /locus_tag="VKQ27_12645"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY89821.1"
                     /translation="MDITLSGQTLGARITGIDLGQTLSDVAFRDILRALGARGVLCFP
                     QQTLTPDQLAAFGRRFGELEINVANVYHEPNHPEMMILSNIRKDGKQIGANDAGQGWH
                     TDMSYSHDIALANILHAQHVPMRNGKPLGETQFRNMHAAYDDLPDELKHKLEGRTATH
                     DFAKFWDMMVAKPGSSRKPLTPEQRAKKPPVSQPIFRRHPITGRYVLYANVGYTMFID
                     GMDPLESGQILDFLFRHQERADYLYAHHWSVGDVLIWDNIGTVHNAVGDYTADEPRYM
                     RRVQVMATLDYGALLAV"
     gene            complement(6450..6977)
                     /locus_tag="VKQ27_12650"
     CDS             complement(6450..6977)
                     /locus_tag="VKQ27_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89822.1"
                     /translation="MIALLAVCTVCGCTGTITSDAPLLASRNASFPLPRHSQIEASTL
                     HDDVWQRDTRKGRIDLVDGAYRVTGPDEPAPSPERYLFRQVGRGEFVAQARNDDAWAY
                     GLIVRADRYYLFTFNRSGENCTSLTAAEQSDLHITVHDDRCYVANLPDLIGLLQHMRK
                     KFPHPTSAFTVSAGG"
     gene            7170..7901
                     /locus_tag="VKQ27_12655"
     CDS             7170..7901
                     /locus_tag="VKQ27_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY89823.1"
                     /translation="MAEFAGKRIVITGGAGGIGIETARTMLAHGAHCVLVDIDEERLG
                     QARETLGTARIATLRSSLETPEACAAALDAAGGPVGALIHLAGLFERDPFDAGEHGVW
                     DRAIAANLTNAYDLAVAFATRYDTRDGPARIVLTSSVAYRRGSAGYPSYAAAKAGLVG
                     LTRSLARKLAPDVLVNAVAPGLIDTRMAVDTIAERGEAYKNEIPVKRYGHPAEVASVI
                     RFLCSRDSSYVNGQTITVDGGITNA"
     gene            complement(7908..8675)
                     /locus_tag="VKQ27_12660"
     CDS             complement(7908..8675)
                     /locus_tag="VKQ27_12660"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY89824.1"
                     /translation="MAGLLEGKSALITGGGGGIGRATSLAFAREGARVAVADASEQTA
                     RETVALINAAGGQAMTLTGDVTNSATVEAMVRSVVTSYGRLDCAFNNAGIAGFQVDAA
                     GKKTADWSEESFDRMIGVNLKGVWLCMKYELPQMVAQGGGAIVNTASIAGLIGLPTSS
                     AYVAAKHGVVGLTKTAALEYADDKVRVNAVCPGYIETNMTADTMRRRGAEIMAKVPFN
                     RMGKPEEIAEMVVWLCSDRASYVSGAAYNVDGGYMAI"
     gene            8741..9355
                     /locus_tag="VKQ27_12665"
     CDS             8741..9355
                     /locus_tag="VKQ27_12665"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY89825.1"
                     /translation="MDQRAMREEILAYKRERILEEAVKLFYERGFTGTTLDDIAAELG
                     VTKPFIYTHFRSKTELLAALCKPTIELSLDAVARGAKSSGTPTERLRRAMVEFTQVVL
                     SRQANIAIYFREEKNLEPAALAEINALRKKFDRVLSNLLSEGVAAGEFELKDVSLAAL
                     AIGGMISWAYTWHRPEGRLPLSEMCEQMADLALQMVGVRTKVAA"
     gene            complement(9468..10415)
                     /locus_tag="VKQ27_12670"
     CDS             complement(9468..10415)
                     /locus_tag="VKQ27_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89826.1"
                     /translation="MSLDPDAETLLAMMRAAGRAPMETLSPDEARQQFRAGRTVTQPD
                     PQEVAEVRDLSCPGPHGAIPLRAYRPMGTAANEVLPVLVYYHGGGWLLGDLDSHDVAC
                     RHYANAAKCRVVSVDYRMAPEYKFPAAVDDCAAATGWVVAQADALGIDRKRVAVGGDS
                     AGGNLAAVMALMARDGDLPAVAFQLLVYPVTDLGMTHESYRRVTEGVPLTSRTMDWFI
                     DHYLPGPKDRADWRASPLRAADLSGVAPALVLTASYDPLCDEGVAYAERLEREGVRVI
                     HLHFSDQLHGFVGQGRIIRAGNMALDMMAAALKRALWGG"
     gene            complement(10427..11209)
                     /locus_tag="VKQ27_12675"
     CDS             complement(10427..11209)
                     /locus_tag="VKQ27_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89827.1"
                     /translation="MSEHETLTVDVNGFAMRVWRKGSGPKLGFLAGFGGLPRWVPFLD
                     ELAKNRTVIVPSLPGFPGGDRAHATLDTHLDWLLATHELLAKAGLDGADLAGSSVGAS
                     MAAEMAALWPASVRRLALIAPFGLFDDAEPPTDPWAQRGDAVPGLMCADPEIWKALKA
                     VPEGANSIEWPIEQTRANEAAARIFWPLGNTKLEKRLKLIQAPTLLVWGEKDSIMPRS
                     YAAKIAKSISGKSETKVIAGAGHLAELDKPAEVAATILGWMN"
     gene            complement(11223..12503)
                     /locus_tag="VKQ27_12680"
     CDS             complement(11223..12503)
                     /locus_tag="VKQ27_12680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89828.1"
                     /translation="MKLMWFHLMPYTELPDDFRDKNPSVWVDIHSSLFDPKRAHLMYN
                     DFMDELEYAAQCGFDAICVNEHHSNGYGLMPSPNLIASSLARRTTDTAICVMGNSLAL
                     YNPPTRVAEEFAMIDVISGGRLIAGFPVGTPMDTCYAYGQNPSMLRQRYLEAHDLVMR
                     AWQEQDTFAFNGRFNQQRYVNIWPRPVQRPHPPVWIPGGGSVETWQWCAEMDYVYCYL
                     SYYGYKAGQATMEGFWNEMARLGKDRNPYRAGFLQFVGVGESRQHAFDLYAEAAEYFY
                     GRCLHVDPRWAVPPGYTSEGSQRAGIESQVKKAADSNVRGKSTGERFASVAREMDAIV
                     DNGYVIVGSPDEVAEQLREVATNLNVGHLMLLLQFGNMSKDLTRYNTKLFAEQVLPKL
                     KDHFAGWEDRWWPQPMEQAQRADIPAFMPKLAAE"
     gene            complement(12557..13189)
                     /locus_tag="VKQ27_12685"
     CDS             complement(12557..13189)
                     /locus_tag="VKQ27_12685"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLY89829.1"
                     /translation="MLDEIATEMRATAQWSGRAALSPQVATALGRVRRSAFVPPESEW
                     AAYQNRPLPIGCGQTISQPYIVALMTELLDLQPTDKVLEIGTGSGYQAAVLAELAPAV
                     YSIETIAELSQLAGQVLISEGCTNVILRVGNGALGWPEEAPFDAIIVTAAAERIPSAL
                     LHQLAPGGRMIIPVGPHGGDQKLILITKDKTGGITQREVLQVAFVPLTDG"
     gene            13278..14453
                     /locus_tag="VKQ27_12690"
     CDS             13278..14453
                     /locus_tag="VKQ27_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY89830.1"
                     /translation="MDSTPAGTDPIARARKLTPVLAAAAPRIDAARELPPDVLDAMHA
                     AGMFRLLVPRALGGAELDPATYIQCVEAIAAGDASVAWCMNQGSGCSMSAGYLQPDVA
                     REMFGGERDVLAWGMGPGAKAIRTDGGWRITGNWAFASGSRHATWLGAHCPCFEADGT
                     SQRYPDGRARERTMLFRRELATIKDIWQVVGLRGTGSDSYAVTDLFVDDAHTLTRDSP
                     EERREPGLIYKFAAMQIYASGFAAVALGTARAALDSFIELARGKTPAQTQAALRDSPV
                     IQSAIGIADAKLNAAHTWLLTVLRNAQDATLLAGEVPTEHRVKIRQASTFAIHQAKEV
                     VDTAYHEAGSTAIFNSNPFERRFRDVNAVTQQVQGRRAHFETIGQHILGANPSLRWL"
     gene            complement(14524..15270)
                     /gene="otsB"
                     /locus_tag="VKQ27_12695"
     CDS             complement(14524..15270)
                     /gene="otsB"
                     /locus_tag="VKQ27_12695"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLY89831.1"
                     /translation="MGSLPPIGRAALLLDLDGTLLDIAPTPDGVVVPDELPGVLRTLR
                     RLLGDAVAVVTGRPVETIDRLLGDAVFAVAGEHGGAVRHAPGAVLERPALPEPPAMMI
                     EGAKRLVAAHPGALFEPKARGFSLHYRAAPKIGEALREGLAALLAGEAAFELLQGSMI
                     WEVRPRGVHKGTAVAALMDRAPFAGKLPVFLGDDVTDEDGIDAAVAMGGAGIRVPEVF
                     GDAAGVRAWLADIAAGRAWPLTDRLSLREA"
     gene            complement(15270..15809)
                     /locus_tag="VKQ27_12700"
     CDS             complement(15270..15809)
                     /locus_tag="VKQ27_12700"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HLY89832.1"
                     /translation="MMGRSFLLMMALGLPAAALAQGMSPITATPLLPPAGTAPQTVPN
                     VAAMPLPAEQTQPPAAQQPAQPGATTATPSGPQPMQLTWVSQGAAQVQVLDKVNAQNT
                     VMTLKVGQQAQYESLTIQVAACSIHPPDQPRDAAAYLKITDNHPDSPGFQGWMMADNP
                     ALSMLESPMYDVRVVGCQA"
     gene            complement(15806..16024)
                     /locus_tag="VKQ27_12705"
     CDS             complement(15806..16024)
                     /locus_tag="VKQ27_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89833.1"
                     /translation="MIPPPPTVWPQIDGAPVSCREKLRMLAENHAELAQAMQDAFEDA
                     VLMGVDEQAMRRILTGMVDGLESPKRAR"
     gene            complement(16021..16533)
                     /gene="mlaD"
                     /locus_tag="VKQ27_12710"
     CDS             complement(16021..16533)
                     /gene="mlaD"
                     /locus_tag="VKQ27_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HLY89834.1"
                     /translation="MRRHSVVEVLVGAIVLIVAAGFLGYALAHSGRTTGSGYDLSARF
                     DHIDGLGVGSDVRLAGVKIGTVTSEQIDPKSFEAVVDFSVRNDIRLPKDSAAIITSES
                     LLGGKYISVQAGADDAMLQPGQRITITQGSVSIEELLGKFIFSMTNAPKPGQAAPASG
                     QTPAAGTTKP"
     gene            complement(16538..16891)
                     /locus_tag="VKQ27_12715"
     CDS             complement(16538..16891)
                     /locus_tag="VKQ27_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HLY89835.1"
                     /translation="MNLGTRLFTWLKGRRVGADSLGNIYYQERSARPGYRVRRWVAYA
                     GAPEASLVPAEWHAWLHYTTDAPLVERARRPWEKPHLPNVTGTPASYRPPGHDYQGGN
                     RARATGDYEAWTPGG"
     gene            17023..18525
                     /locus_tag="VKQ27_12720"
     CDS             17023..18525
                     /locus_tag="VKQ27_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HLY89836.1"
                     /translation="MKYPRTWQGVRMRRVMAHPDPDAPQRQVTLPAAWDDRAASALAE
                     LAPGGAPVMLAGAAEAWIAPIAERALRAGLETPLAERLHRMLLLRHGAPSLSVWRGNA
                     GAEPGFVLDLASFHDAAIGFDAPAFAEAVETAVTALTLFAPAATRISVGMADLAGLLA
                     LLGIEYGSDDSLATTRAVAAILRGRAEAASGAMARLFGSVAPAALDWPAPPAETPVPG
                     LAEAARAARLAAAGFDGLRHTALTAVAAPAAADALLGVETGGIAPAFSPLDETGALNR
                     TARAWLAARHITPEAALARLLAGNDPLPAASVTGAIAMHDAVSPFVHTMPARPEPLRQ
                     PMHTVRRRELPTRRAGYTQKAAVGGHRLYLRTGEYDDGSLGEVFIALHKEGAAFRGLM
                     DNFAVAISLGLQHGVPLEAFVEAFTFTRFGPAGAVEGDPAVAHATSLLDYTFRHLAAN
                     YLGRRDIPEAEIEEADTVGNGSRDHAPLLPLDLPAESPRARRRGFKVVSR"
     gene            18589..19056
                     /locus_tag="VKQ27_12725"
     CDS             18589..19056
                     /locus_tag="VKQ27_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY89837.1"
                     /translation="MPSRVELKLAELGITLPSPMPPIANYVPYVVTGDLVVVSGQVPA
                     VDGKIAITGKVSYGVSLEQGREAARLCFINLLVHLKAACGGDLDRVKRVVRLGGFVAS
                     PPEFRDHAQVMNGASDLAVAVFGDAGRHARTTIGVPALPADAAVEVEGMFEIS"
     gene            19066..20229
                     /locus_tag="VKQ27_12730"
     CDS             19066..20229
                     /locus_tag="VKQ27_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY89838.1"
                     /translation="MPDGAATLTLTLHQKIADVPAADWDACAGPGNPFVSHAFLSAME
                     DSGSASARTGWLPQHAVLRGEAGQVVAVAPMYAKSHSYGEYVFDHGWAHALERAGGDY
                     YPKLQVAAPFSPVPGPRLLRRPDSGIPVAALASALEQACASLGVSSVHITFCAEDEWQ
                     QLGDAGWLQRVGMQFHWENAGYATFDDFLGSLSSRKRKVLRRERRDANAAGLTFHALS
                     GADIKEAHWDAFYRFYRSTVDRKWGSAYLSRQFFSLLGERLGDRVVLMLADNAGKPVA
                     GALNLAGDEALYGRNWGCRGDWPFLHFELCYYRAIDWAIEHKLPRVEAGAQGRHKIQR
                     GYLPKPTYSAHWIAHTGLRRAIANFLEDERLGIHAEMAHLAEQSPFRKEDETD"
     gene            20326..21165
                     /locus_tag="VKQ27_12735"
     CDS             20326..21165
                     /locus_tag="VKQ27_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLY89839.1"
                     /translation="MLSAHVVAIVLLAATLHAGWNVLVRSGRDRRQETALLLIGGTFW
                     AVVLLPFLPGLPLASWPYLLISSVLNGVFFILIAEAYSRGGIALAYPVMRGVAPMLTL
                     VAAWLVLHEVLPTAGWFGVAAICGGVVLLARGRGDPEEGRAVRFALANAVVISAYTLN
                     DAIGVRIAQEPLSYTLWMFPLSAVPTWLWLRHRTSLHWPAPREALRGIGGAGASIGAY
                     ALVLWAITQAPVAPVAALRESSILIGVVLARVLLGEQPGRRGWGGAVAIAVGAAILRL
                     APG"
     gene            complement(21182..21937)
                     /locus_tag="VKQ27_12740"
     CDS             complement(21182..21937)
                     /locus_tag="VKQ27_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY89840.1"
                     /translation="MPTWSPPRVAHDAASLRFVLAPARLVRGHRVYAIGDIHGCLDEL
                     QALHAAVADDFKRNQPVSAVLVHLGDYIDFGPDSAGVIELLASGPPVAGLRTVNLMGD
                     HERTALDALSGDGAAATDWLHTGGEAALRSWGIGVEVRRTDWVRFVPEAHVAFMQGLE
                     PAYRAGGYLFVHAGVRPGVALEKQAEDDLLGIRQSFLASEQDFGAVVVHGHSAAPTPV
                     MKENRIGIDTGAGCGGRLTCAVFEEDGVAFIAA"
     gene            22087..22569
                     /locus_tag="VKQ27_12745"
     CDS             22087..22569
                     /locus_tag="VKQ27_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HLY89841.1"
                     /translation="MAKITFPVEAGHIMLFARAVGDYNPVYHDAEAAKKSEVGGIIAP
                     PTFPQAMAQFDPDYPLRWRPGQPWFGSGKTPSGVTERASGGGGLHAEQHFEYHRPLRP
                     GDVLTVETKPGNTWERESKRAGKLTFRERIAEYRDQKGELVITARSVGVTTERPVERS
                     "
BASE COUNT         3935 a         7567 c         7171 g         3997 t
ORIGIN      
        1 gaacgcggcg tcttcggcgg cctgaccgtg gaggagaacc tgcgcatcgc cgcgctcacc
       61 gcacctgcgc cctggggact cgaccgtatc tacgcgatgt tttcccgcct cgccgaacgg
      121 cgccgttcgc gtggcggcag cctgtcaggt ggtgaacagc agatgctgac aatcgcccgc
      181 gcgctgatcc gtgacgcgcg catcatcctg ctggacgaac cgttcgaagg actggcgccg
      241 ctgatcgtgc gcgaccttgt gcggatcagc cgcgagcttg cggcgcaagg gcgcacgatc
      301 attgtggtgg agcagaacgt ggtggccgcg ctcagcttcg ccgaccgggt gtatatcctg
      361 aacaatgggc acgtggtgta cgagggcacg ccagactcgc tgcacgcgaa ccccgaggtg
      421 atgaagagtc acctcggggt ctgaagcggc gtcagtagtg accccagtag ccgggggcgc
      481 caaagagcag gaacaggacc aacagcagca ggatcaggcc gaacccgccg ccatagtgag
      541 cgccgccgta gtagccggac cggtagccgt agtatccgcc gccaccgaac agcagcacga
      601 taatcaggat cagtagtaac aggcccatga tgcggtgcct ttcccctgtg gtgtcgacca
      661 ctttggagcg ccgcaaggtc acggcgtggc acaatcatgg cgatgcggcg gcattgccca
      721 ttctgacgta agcttgaccg catgccgcag ccaacctggg accctgatag ccttgctgcc
      781 cgcctgccat tcctgcaccg ccgtgcgctg ctgacggcgg gcacgcgcgc gtattttact
      841 gcccgcggct atacagaggt ggagacaccc tttgccgtgc cggcgcccgg ggaggaggtt
      901 cacctgcgcg ctttccgcac cgagcgcgaa tatccggacg gcgccagcaa gccgctctgg
      961 ctgcacacca gtccagaatt cgccatgaag aagctgctgg tggcgggagc gggaccgatc
     1021 taccagttgg cgcgcgtctg gcggaacggc gagggaagcg atctccacgc accggagttc
     1081 accatgctgg aatggtatca ccccggcgcg accatggact cactgattga tgaaaccacc
     1141 gcatatctgc gtgccgtgtt gccgcctgtc gtgacatgcc gtggtgttac caccgacctc
     1201 tcgcgcatcg agcgtatgac ggtcgccgac gcattcacgc gctacgtcgg tgcggatgtc
     1261 ctggccgccg cggatgacgc accgtcgctc gccgccgccg ctgggacccg gctgcgtgaa
     1321 ggcgagacct gggaggacct gttcttccgt ctgttgctgg agcgcgtgga accgaacctc
     1381 ggccgcgcgc accccacgtt cctgacacat tggcctgccg cacaggcagc cttggccagg
     1441 cgcgatccgg cggatccgcg cgtcgctgaa cgcttcgagc tgttcgtctg tgggatcgag
     1501 ctggccaatg cctttgtcga actgaccgac gcggatgaac agcgcgcgcg cttcgagtcg
     1561 gaccgggcgc ggcggcatgc catttccggg gaggcctggc cgctcgatga agacttcctg
     1621 gctgctctta ccttcgggat gccgccatcg gccgggatcg ccctcggctt cgacaggctg
     1681 gccatgatcg catcaggagc aaaccgcatc gatcaggtat tgtggctgcc ggaaccggac
     1741 taagtacagt cgcggttagc cctggagtgc gtccgatgcg ttccgccgcc ttgttgttgc
     1801 tggctgggct gcttggcggc tgtgccaatc aattggcaga gcggcaagcc tatctgaagc
     1861 agttcatagg ccagcccgag tcatcggtcg tgcaggcgat gggagttccg tcgcgcacct
     1921 tccagactgg cggggtcacg tatctggcct acaatgaaca ccgcatagac tttatcccgg
     1981 gcacgccaac ttatgcgcca tcttatctcg gctcttacgg cgctttcccg ccgcagatcg
     2041 tacagcttga ctgcgagaca acattccagg tcgtggatgg caaaattgct tcattccagc
     2101 ttcgcggcaa cgcctgcggc tgaaaatccg ttgcttggtc ggtgtgttct aagcgcggtg
     2161 tcggctatcg gttctggacc agcagcagcg ccggcaaagc caactgcgca atcaggcgca
     2221 tgcgcggcat gtggctctcg tcgtaatagc cggcgcgatg gcagaggttg agcgtaccca
     2281 ttgtctggcc tcgccagcgc accggcatgt tcaggatgct gtcgcagccg agcgaatgga
     2341 tcagctcgtg gtcgaagaag ttggcggcaa tgtcctcgcg ggtgcggccg atatacggat
     2401 tgccgccgtg gatgacgcgc tgcatccacg gtgagtcggt caccggcttc cggccgccga
     2461 tgggataggc ttcgggcttg ttggtgtaga agcgttcgga ctggcgaagc gctgcgtggt
     2521 gaacaagaac ggtgaatagg atgtggccgg gctcgcgtgc cagcgccgca tcgatggcgc
     2581 ggaacgtcgc cagaggctga tcagtgtcgg cgtgcgcggc gaggatcgcg tcggtcattc
     2641 accgccctcc gcagataacc agcccgacaa ttgatccggc ttgcttatcg gaaaatcgag
     2701 tgcggccctg gggcggcgag ggcaggggcg agcacgacgc cttcgctcct cgttgcagtc
     2761 gatgcgccct ttcgttcgct ggcgccagcc cgaggcgcgg cttcgccgcc tccggcgccg
     2821 agcatccgcg acagcttctg ggccgcatcc agcaccatgg gcaacaaggc ccgaggccga
     2881 ggcggtcgca tccgcatggt cggggcgctg accgacaaca cagccaccac ctgccccgat
     2941 gcgtcgcgta tcggtgccgc cacggtcgcc gccccggcga cgacttcgtc cgtcgtcatc
     3001 agatagccac gtgtgcggat gcgctgcagg tcggcggcaa tccaggccga gtcagtgcgg
     3061 gtcgccggcg tgaaacgcgg gagccgctgc gccagcacct gcgtctgcac cgcctcgggc
     3121 gcgtgcgcga gcaacaggcg gctggatccg gcgtggaggg tctggcgctt gccgacctcg
     3181 ctgtacagcc gcagccctgg atcagtttgg tagatggcga tcgtctcgct ctccagcccg
     3241 tcacggaccc gcaaatacac gttctcgccg ctggtcttgc cgagtgcggc caggaacggc
     3301 attgccgtcg ccgccaacgc cccctgctcg acagccgccc gtcccagcac gccccagcgt
     3361 gcgccgagcc gccacatccc gcgggcctcg tcctggatgg caaagccgcg tgcttccaac
     3421 gtccggagca gccggaatcc ggcggtgcgg gtgcatcgcg ctgcgtccgc cagcgcggct
     3481 aacggcgctg ggccgatgcg cgacagcgcc tcaaggagat ccagcgcccg cccgacggca
     3541 gcgatgctgt agtcgcgggc cggtgaggtg ccggacccat tcgatccgct gttggagcgg
     3601 gcacgcggac gggtcttccc cttggtgccc ttgctcttag gctgggccat acgggttcct
     3661 aggtgaaagt atcggttgat cgcagattag cacggccatc cgttgttgag catggggaca
     3721 aggtgaacaa attgggtgaa ttgcgcgcaa aatcctcggg gcgagcggtg cacaactccg
     3781 tggacaatga cacccggcat cgcatgcgtc aaaaggcagc ggcccggatg tggtcgccag
     3841 gagcctatct aagcgaagat taatacggat aaatccacta cacatccata tattcggcgc
     3901 ggacctgggc tgcgattcgc ggaagcgtct ccgggtgcgc gtcggcggcc gccgtgagct
     3961 tgtggcccaa tcaaggctgc gctaattccc cgcgttcgca tcggaaatga cgggccgcag
     4021 ggcttgccgg cgcgggcgtg gtggaattgg cagacacgcc agatttaggt tctggtggcg
     4081 caagccgtgg gggttcaagt ccctccgccc gcaccagcag gcggacggac aggcacagga
     4141 ttgatcgaat gcaggttacc gaaaccctct ccgagggtct gaagcgcgcc tacaccgtgg
     4201 tggttcccgc ggccgacctc gagaccaaac gcgcggcgcg gctggccaat ctcagcaaga
     4261 cgctgacgtt gcccggattc cgccccggca agatcccgcc caacatcgtc aagcagcgct
     4321 ttggcacggc tgtcggcgcg gaagtgctgg aggagtcggt cagcgaagca acgcagcagg
     4381 tgctcagcga acgcggcctt cgcccggcgc agcagccgaa ggtggatgtg gtcagcctcg
     4441 atcccaccgg cacaaccggc aaggacctgg aatttaaggt cgaactggaa cttttgccgg
     4501 aggtcacgcc gcccgacttc tcggcgatca agctgacccg cctgaagacc gagcccgatg
     4561 gcgagaccat cgacaagggt ctgaacgaaa tcgcccagcg caaccggacg ctggaggagg
     4621 tcccggccga ggaactcggc gagcgcggcg ccgcgacggg cgaagtcctg acggtcgact
     4681 tcaccggcaa gatggacggc accgaattcc cgggcggtgc tggcaaggac atcgaggtcg
     4741 agatcggcgg ctccaacttc atcccgggct tcagcgagca gctcgagggg atgaagccgg
     4801 gcgagacgcg cacgatcgag gtgaccttcc ccgccgacta cggtgtcgcg acgctcgccg
     4861 gcaagccggc tacctttgat gtgactgccc acaagatcag ccgcggcaac gtccccgcgg
     4921 tggacgacga actggcgacg aaactcggct tcgaggacct ggccgagatg cgcgacacga
     4981 tcaccaagcg cttccagtcg gaatatgatc agttgtcgcg cctgcgcctg aagcgccagt
     5041 tgctcgatgc cttggctgat accgtgaagt tcgagtcacc ggaaggcatg gtcaatcagg
     5101 agttcgacca gatctggcag cggctggaga gcgagcgcca gcagggccgg ctggatgacg
     5161 acgacaagga taaggacgaa gagacgctgc gctctgacta tcgcgcgatc gccgagcggc
     5221 gcgtgcgcct ggggctgctg ctggcggaaa tcggccgtat caacagcatc actgtgcagc
     5281 ccgatgagat gacacgcgcg atgcgcgctg aggcgatgcg ctttcccggc caggaacagc
     5341 aggtgttcga gttcttccgc cagaacccgc gcgcgactga ctcgttgcgc gggccgctgt
     5401 tcgaggagaa ggtgatcaac ttcatccttg agctggccca ggtcgaggac cagacggtgt
     5461 cgctcgaaga actgaccaag gagccgccgt ccccagtctg aagtggacag gagatccgcg
     5521 atggacatca ccctcagcgg ccagacgctc ggagcccgga ttaccggcat cgatctcggt
     5581 cagacgcttt ccgacgttgc ctttcgcgat atcctgcgcg cgctgggggc gcgtggcgtg
     5641 ctgtgcttcc cacaacagac gctgacaccc gatcagcttg ccgcgttcgg gcgcaggttc
     5701 ggtgagctgg agatcaacgt cgccaacgtc tatcacgagc cgaaccatcc cgagatgatg
     5761 atcctgtcca acatcaggaa ggacgggaag cagatcggcg ccaatgatgc cggccagggc
     5821 tggcataccg acatgtccta tagccacgat atcgcgctgg cgaacatcct tcatgcgcag
     5881 catgtgccga tgcgcaacgg caagccgctg ggcgagacgc agttccgcaa catgcatgcg
     5941 gcgtacgacg atctgccgga tgagttgaag cacaagctgg aagggcggac ggcgacgcac
     6001 gacttcgcca agttctggga catgatggtc gcgaagcccg gctcctcacg gaagcctctg
     6061 acgcccgagc agcgcgcgaa gaagccgccg gtgtcgcagc cgattttccg gcggcatccg
     6121 atcaccggac ggtatgttct gtatgccaat gtcggctaca cgatgttcat cgatgggatg
     6181 gatccgctgg agagcggcca gattctcgac ttcctgttcc gccaccagga gcgcgccgac
     6241 tatctgtatg cacatcactg gtcggtgggc gacgtactga tatgggacaa tatcggcacc
     6301 gtgcacaacg ccgttggcga ctacacggcg gatgaacctc ggtatatgcg acgggtgcag
     6361 gtgatggcga cgctggatta cggtgcgttg ctggctgtct gaacgaccga ccagtgcctg
     6421 caaatcgcgc gctcgctgaa cgccgtcact tatccgcctg cactgacagt gaaagcggat
     6481 gtcgggtgcg gaaacttctt gcgcatgtgc tgcaagaggc caattagatc gggcaggttc
     6541 gccacgtagc agcgatcatc atgaacggtt atatggaggt cactttgctc cgcagccgtt
     6601 aggctggtgc aattctcgcc gctccgattg aaagtaaaca gatagtatct gtccgcccgc
     6661 acgatcagcc catatgccca cgcgtcgtca ttccgtgcct gggcgacgaa ctcaccccga
     6721 ccgacctgcc tgaacagata tctctcgggc gatggtgccg gctcgtccgg accggtcaca
     6781 cgatatgccc catccacgag atcgatacgg cctttccttg tgtcacgctg ccaaacatcg
     6841 tcgtgcaatg tgcttgcttc aatctgggaa tgacgtggca acggaaacga ggcattcctg
     6901 gatgccagca aaggggcatc gctggtaatc gtgccagtac acccacacac cgtgcagacc
     6961 gcaagcagcg caatcaccgc ccgcatccct gtcacccccg tgtttggcat cgtatcgtat
     7021 tagatacctc cgagcgatga agcaccaggg ctcgcggcac gggatgacgg ttaacgctga
     7081 attggagtat ggattgacaa acagcatgac ggagacagag cccgtgcaag cgatgccaat
     7141 gctggcttct acaccccagg agcactgaca tggcggagtt tgccggtaag cgcatcgtca
     7201 taacgggcgg cgccggaggt atcggcatag agaccgcgcg cacaatgctg gcgcatggcg
     7261 cgcattgcgt gctggtggac atcgacgagg aacgcctggg gcaggcacgc gaaacgcttg
     7321 gcacggcgcg gatcgcgaca ttgcggtctt ccctggagac gcctgaggca tgtgctgccg
     7381 cgttggatgc ggccggtggg ccggtgggcg cattgatcca tcttgcaggc ctgttcgagc
     7441 gtgatccgtt cgatgcaggc gaacatggcg tatgggatcg cgcgatcgcc gccaatctga
     7501 ccaatgccta cgatctcgcg gtggccttcg ccacgcgcta cgacacgcgc gacgggccgg
     7561 cgcggatcgt gctgacgagc tcggtcgcgt atcggcgtgg cagtgcggga tatccgtcgt
     7621 acgccgctgc aaaagcggga ctggttgggc tgacgcggtc gctggcgcgg aagctggcgc
     7681 cggatgtgct ggtgaacgcc gtcgcgccag ggctgatcga cacgcgcatg gctgtagata
     7741 ccattgctga gcgcggcgag gcgtacaaga acgaaatccc cgtgaagcgc tatgggcacc
     7801 cggccgaggt ggcatcggtg atccggttcc tgtgttcgcg cgactcgtcc tatgtgaacg
     7861 gccagacgat cacggtcgat ggcgggataa cgaacgcctg aggatggtca gatcgccata
     7921 tagccgccgt ccacgttgta ggccgcgccg ctgacatacg atgccctatc cgaacacagc
     7981 cagacgacca tttcggcgat ttcctccggc ttgcccatgc ggttgaacgg caccttggcc
     8041 atgatctcgg caccgcgacg gcgcatcgtg tccgccgtca tgttcgtttc gatatagccg
     8101 gggcagaccg cattcacgcg caccttgtcg tccgcatatt ccagcgccgc cgtcttggtc
     8161 aggcccacca cgccatgttt tgcggcgaca taggccgacg aggtaggcag cccgatcaac
     8221 ccggcgatgg aggctgtatt gacgattgca ccgccaccct gggcgaccat ctgcggcagc
     8281 tcgtacttca tgcacagcca gacgcctttc aggttgacgc cgatcatccg gtcgaaggac
     8341 tcctcggacc aatcggcggt cttcttgccg gccgcatcga cctggaaccc ggcgatgccc
     8401 gcgttgttga aggcgcaatc cagacggcca tagctggtga cgacggatct caccatcgct
     8461 tcgaccgtgg ccgagttggt gacatctccg gtcagcgtca tggcctggcc gccggcagcg
     8521 ttgatcagcg cgaccgtctc acgcgccgtc tgctccgacg catcggcgac ggcgacgcgt
     8581 gccccttcac gcgcgaaggc cagtgaggtg gcgcggccga tgccgccgcc tccgcctgtt
     8641 atcagcgctg atttgccttc cagtagaccg gccattgttc cctccttggc tgcatttctt
     8701 ccaattaccc ggcggaaagg ctaacataac aagctgcaca atggaccagc gggcaatgcg
     8761 cgaggagata ctggcctaca agcgcgagcg aattctcgag gaagcggtca agctgttcta
     8821 tgagcgcggc ttcaccggta ccactctcga tgacatcgcc gctgaattgg gggtcacgaa
     8881 gccgttcatc tatacgcatt tccgcagcaa gacggagctg ctggcggcgt tgtgcaagcc
     8941 gacgatcgag ttgtcgctgg atgcggtcgc gcggggcgcg aaaagctctg gcacgcctac
     9001 cgagcggctg cggcgtgcga tggtagagtt cacccaggtg gtgctgtcac ggcaggccaa
     9061 tatcgcgatc tacttccgcg aggagaagaa cctggaaccg gcggcgctgg ccgagatcaa
     9121 tgcactgcgg aagaagttcg atcgggtgtt gtcgaatttg cttagtgagg gcgttgccgc
     9181 cggcgagttc gaactgaagg acgtcagcct ggctgcgctg gcgatcggcg ggatgatctc
     9241 gtgggcttat acgtggcacc gtcccgaagg gcgcctaccg ctctccgaga tgtgcgagca
     9301 gatggccgac ctggcgctgc agatggtggg cgtgcgcacc aaggtcgctg catagcggaa
     9361 aacggaattg gaagcgcaaa gcacgcaaaa gacgcttgtg ccctagccgc gccctttgcc
     9421 gcgcagccat catgctttgc gtgtcttgcg gtttaaattt cttccttcta gccgccccac
     9481 agggcacgct tcagcgctgc tgccatcata tccagcgcca tgttccccgc ccggatgatg
     9541 cgaccctggc cgacaaagcc atgcaactga tcggagaagt gcaggtggat cacccgcacg
     9601 ccttcccgct ccagtcgctc ggcatacgcc acgccttcat cacataacgg atcatagctc
     9661 gcggtcagca caagcgccgg tgcgacgcca gaaagatccg ctgcccgtag cggcgaagcg
     9721 cgccaatccg ctctatcttt cggtcctggc aagtagtggt cgatgaacca gtccatcgtc
     9781 cgtgatgtga gcggcacgcc ctcggtgacg cgcctgtacg actcatgggt catcccaagg
     9841 tcagtcaccg ggtagaccag caattgaaac gccaccgcgg gcaggtcgcc gtcgcgcgcc
     9901 atcagcgcca tgactgccgc caggttgccg cccgccgaat ctccgcccac cgccacgcgc
     9961 ttgcgatcga tccccagtgc atcggcctgc gccaccaccc agccagtcgc cgctgcgcaa
    10021 tcatccaccg ccgcagggaa cttgtattcc ggcgccatcc ggtaatcgac cgacaccacc
    10081 cgacacttcg ccgcattggc atagtgccgg cacgcgacat cgtgcgagtc gagatcgccc
    10141 agcagccagc cgccgccatg gtagtagacc agcaccggca gcacctcgtt cgctgccgtg
    10201 cccatcgggc gatatgcccg cagcggaata gcgccgtgcg gtccggggca cgacaggtcg
    10261 cgcacctcgg ccacttcctg cggatcgggc tgcgtcaccg tgcgcccggc gcgaaactgc
    10321 tggcgcgcct cgtcgggcga gagcgtctcc atcggcgcgc ggcccgcggc acgcatcatc
    10381 gccagcagcg tttcggcatc cggatcgagt gacatgcgca cgctcctcag ttcatccagc
    10441 ccagaatggt ggccgcaacc tcggcgggtt tgtcgagttc ggcaagatgc cccgctcctg
    10501 cgatcacctt cgtctccgac ttgccgctga tgctcttggc gatcttcgcc gcgtagctac
    10561 gcggcatgat cgagtccttc tcgccccaga ccagcaatgt tggtgcctgg atcagcttca
    10621 ggcgcttctc cagcttggta tttcccagag gccagaagat ccgcgccgcc gcttcgttcg
    10681 cgcgcgtctg ttcgatcggc cattcgatcg aattcgcccc ttcgggcacc gccttcagcg
    10741 ccttccagat ctccggatca gcgcacatca gtccgggcac tgcatcgccc cgttgtgccc
    10801 aaggatcggt gggcggctcc gcatcgtcga acaggccgaa cggcgcgatc agcgccagcc
    10861 gccgcacgct tgctggccac agcgctgcca tctcagctgc catcgacgcg cccaccgaac
    10921 tgcctgccag gtcggcgccg tccagcccgg cctttgccag cagttcatgg gtcgccagca
    10981 gccagtcgag atgcgtgtcc agcgtcgcat gtgcgcgatc gccgccggga aagccgggca
    11041 gcgacggcac gatcaccgtg cggttcttgg ccagctcatc gaggaacggc acccagcgcg
    11101 gcagcccacc gaagccggca aggaagccga gcttcggccc gctccccttg cgccagacgc
    11161 gcatggcaaa accattgacg tcgacggtca acgtctcatg ttcgctcacg gttcgctcct
    11221 gtctattcgg cggcgagttt cggcataaag gccgggatgt cggcgcgctg agcctgctcc
    11281 atcggctgcg gccaccagcg atcctcccag ccggcgaagt ggtccttcag cttcggcaac
    11341 acctgttccg cgaacagctt ggtgttgtac cgcgtcaggt ccttgctcat gttgccgaac
    11401 tgcagcagca gcatcaggtg gccgacgttc aggttggtcg cgacttcgcg caactgctcg
    11461 gcgacttcat caggcgagcc gacgatgaca taaccgttgt ccacgatcgc atccatctcg
    11521 cgcgcgaccg aggcgaaacg ctcgcctgtg gatttgccgc gcacgttgga gtcagcggcc
    11581 ttcttcacct ggctttcgat gccggcgcgc tggctgcctt cgctggtata gcccggcggc
    11641 accgcccacc gcggatcgac atgcaggcag cggccgtaga aatattccgc agcttcagca
    11701 tagagatcga aggcgtgctg gcggctttcg ccgacgccga cgaattgcag gaagccggcg
    11761 cggtacggat tgcggtcctt tcccagcctc gccatttcgt tccagaagcc ttccatcgtg
    11821 gcctggcccg ccttgtaccc gtagtagctg aggtagcagt aaacgtagtc catctccgcg
    11881 caccactgcc acgtctccac cgagccgccg cccggtatcc acaccggtgg atgcgggcgt
    11941 tgcacgggac ggggccagat gttcacgtag cgctgctgat tgaaccggcc gttgaacgcg
    12001 aacgtgtcct gttcctgcca ggcccgcatg accaggtcgt gcgcttccag gtagcgctgg
    12061 cgcagcatcg aagggttttg accgtaggcg tagcaggtgt ccatcggtgt gccgacggga
    12121 aatcccgcga tcaaccgccc acctgaaatc acgtcgatca tcgcgaattc ttcggcaacg
    12181 cgcgtcggcg gattgtagag ggcgagcgag ttgcccatca cgcagatcgc cgtgtcggtc
    12241 gtgcggcgcg cgagtgagga ggcgatcagg ttcggcgaag gcatcaggcc atagccgttc
    12301 gagtgatgct cgttcacgca gatcgcatcg aagccgcact gtgccgcata ctcgagctca
    12361 tccatgaaat cgttgtacat gaggtgcgcg cgcttcgggt cgaacaacga ggagtggatg
    12421 tccacccaca ccgacggatt cttgtcccgg aaatcgtccg gcagttccgt gtatggcatc
    12481 aggtggaacc acatcagctt catgctcgtt ctccgcttgt tctaccgacc agtatccgtt
    12541 ctatcttgtc ggtaagtcaa ccgtcggtca gtggcacgaa cgcgacttgc agcacttccc
    12601 gctgggtgat tccacctgtc ttatcctttg tgatcaggat gagtttctgg tcgccgccat
    12661 ggggaccaac gggaatgatc atgcgtccgc caggggcaag ctgatgcagg agggcggagg
    12721 ggatgcgctc ggccgccgcg gtgacgatga tggcatcgaa cggtgcttcc tccggccagc
    12781 ccagggcccc gttgcctacg cgcagtatga cgttggtgca gccttccgat atcagcacct
    12841 gacctgcgag ctgcgacagt tcggcaatgg tctcgatcga gtacactgcc ggcgcaagtt
    12901 cggccagaac cgccgcctgg tagccggagc cggtgccaat ctccagcacc ttgtctgtcg
    12961 gctggaggtc caggagctcg gtcatcagcg ccacaatata tggctgcgat atcgtctgcc
    13021 cgcagccgat cggcaggggt cggttctgat aggctgccca ctcagactcg ggtggcacga
    13081 aggcactgcg gcgcaccctg ccaagggctg tggccacctg cggcgacaac gcggcgcgcc
    13141 cgctccactg cgcggtggcg cgcatttcgg ttgcgatttc gtccagcata gcggcgcgct
    13201 catccatggg gggcgatttt gccgttcgcg gtgccgacag tatagcgtgc gcagctacac
    13261 gaaccgaggg atgcttcatg gattcgacac cggccgggac tgacccgatt gcccgcgccc
    13321 gcaaactgac cccggtcttg gccgcagccg cgccacgcat cgatgcggcg cgcgaactgc
    13381 cgccagacgt actggatgcg atgcatgcgg cgggaatgtt ccgcctgctg gtcccgcgcg
    13441 cgctgggcgg ggccgagctg gatccggcca cgtatataca atgcgtggag gcgatcgccg
    13501 ccggcgatgc gtcggtggcg tggtgcatga accaaggctc cggctgttcg atgtccgccg
    13561 gctatctgca acccgatgtt gcgcgcgaga tgttcggcgg tgagcgggac gtgctggcct
    13621 ggggcatggg acccggcgcc aaggcgatcc gcaccgacgg gggctggcgt atcaccggca
    13681 actgggcgtt cgccagcggc agccgccacg caacgtggct cggcgcgcat tgcccgtgct
    13741 tcgaggccga tggcacgtcg cagcgctatc ctgacgggcg cgcgcgcgaa cgcaccatgc
    13801 tgttccggcg ggaactcgcg acaatcaagg acatctggca ggtggtgggt ctgcgcggta
    13861 cgggcagcga ttcctatgcg gtgaccgatc tcttcgtcga tgacgcgcat acgctgacgc
    13921 gcgacagccc ggaggagcgt cgtgaaccgg gcctgatcta caagtttgcc gcgatgcaga
    13981 tctatgcgtc gggctttgcc gctgttgcgc tcggcaccgc gcgtgcggcg ctggattcct
    14041 tcatcgagct ggcacgtggc aagaccccgg cgcagacgca ggctgcgttg cgcgacagcc
    14101 cggtgatcca gtcagccatc ggcatcgccg acgcgaagct gaacgccgcc catacctggc
    14161 tgctgacagt gcttcggaat gcgcaggacg cgacgctctt ggctggcgaa gtgccgacgg
    14221 agcaccgcgt gaagatccgg caggcatcga cgttcgcgat ccatcaggcg aaggaggtgg
    14281 tggacactgc ctatcacgag gctggctcga ccgcgatttt caacagcaat ccgttcgagc
    14341 gccgcttccg cgacgtgaat gccgtcaccc agcaggtgca gggccgccgc gcacatttcg
    14401 aaacgatcgg ccagcatata ctgggggcta acccaagcct gcgatggctg tgaagtaaac
    14461 ctcggacagt gtcattgcga gaagcgtagc gatgaagcaa tcccgaacga gattgcttcg
    14521 tcgctacgct tctcgcaatg acaggcgatc cgtcagcggc cacgctcgcc cggccgcaat
    14581 gtccgccagc cacgcccgca cgcccgccgc atcgccaaat acctctggaa cgcggattcc
    14641 tgcaccgccc attgccacgg cggcatcgat cccatcctca tcggtcacgt cgtcacccag
    14701 aaacaccggc aactttccgg caaacggtgc gcgatccatc agcgcggcaa cggccgtgcc
    14761 cttgtgcacg ccgcgcggcc gcacttccca gatcatgctg ccctgaagca actcgaacgc
    14821 cgcttcgccg gcaagcagcg ctgccagccc ctcgcgcaac gcttccccaa tctttggcgc
    14881 cgcgcggtaa tgcagcgaga accctcgcgc cttcggctcg aacagcgcac caggatgcgc
    14941 cgccaccagc cgtttcgcgc cttcaatcat catcgccgga ggttccggca gcgccggccg
    15001 ttccagcacc gcgcccggcg catgtcgcac ggcgccacca tgctcaccag ccaccgcaaa
    15061 caccgcatcg ccgagcagcc gatcgattgt ctctaccggg cgtccggtca cgaccgccac
    15121 cgcatcaccg agcaggcgcc gcagcgtccg caggacaccc ggcaactcat ccggcaccac
    15181 cacgccatca ggggtcggcg cgatatccaa caatgtcccg tccaggtcga gcagcagcgc
    15241 cgcccgcccg attggcggca gagaacccac taagcctggc accctacgac gcgtacgtcg
    15301 tacatcgggc tctccagcat cgaaagcgcc ggattatcgg ccatcatcca tccctgaaat
    15361 ccgggtgaat cggggtggtt gtcggtgatc ttcaggtagg ctgccgcatc ccgcggctgg
    15421 tcaggcgggt ggatggagca cgccgccacc tggatggtca gcgactcata ttgagcctgc
    15481 tgtccgacct tcagcgtcat caccgtgttc tgcgcattca ccttgtccag cacctgcacc
    15541 tgagccgccc cctgcgatac ccatgtcagt tgcattggct gtggcccgga aggggtcgcc
    15601 gtcgtcgctc ccggttgcgc gggttgctgc gccgctggcg gctgggtctg ctccgctggc
    15661 agcggcatcg ccgctacatt cggcaccgtc tgcggagcgg tccccgccgg aggcagcaac
    15721 ggtgtggcgg tgatcggtga catgccctgc gccagcgccg cagcgggcag cccgagcgcc
    15781 atcatcagaa ggaaactcct ccccatcatc gcgccctctt cggcgactcc agtccgtcga
    15841 ccatgcccgt caggatacgc cgcattgcct gctcatcgac acccatcagc actgcgtcct
    15901 cgaacgcgtc ctgcatcgcc tgtgccagtt cggcgtgatt ctccgccagc atgcgcagct
    15961 tctcgcggca cgataccggc gccccgtcga tctgcggcca gacggtgggg ggagggggga
    16021 tcatggcttc gtggtgcccg ccgccggcgt ctggcctgat gcgggcgctg cctgcccagg
    16081 cttcggggca ttcgtcatgc tgaagatgaa cttgcccaaa agttcctcga tgctgaccga
    16141 tccctgagtg atggtgatcc gctgccccgg ctgcaacatc gcgtcatcag cgccagcctg
    16201 aacggagatg tatttcccgc ccagcaaact ctcgctggtg atgatcgcgg cgctgtcctt
    16261 cggcagcctg atgtcattcc gcaccgagaa atccaccact gcctcgaacg acttcggatc
    16321 gatctgctcg gacgtcactg tgccgatttt cactcccgcc agccgcacgt ccgaccccac
    16381 gcccaacccg tcgatatgat cgaatcttgc cgacagatca tagccggagc cggtggtgcg
    16441 gcccgaatgc gccagcgcat atccgagaaa ccccgctgcc acgatcagca cgatggcccc
    16501 gaccaggacc tcgacgacgc tatgccgtcg catgggatca gccgcccggc gtccaggctt
    16561 cgtagtcgcc tgtggcgcgc gcccggttgc ccccctggta atcgtgccca ggcggccgat
    16621 agctggccgg cgtgccggtc acgttgggca gatgcggctt ttcccaaggg cggcgcgcgc
    16681 gctctacaag tggagcgtct gtcgtgtagt ggagccacgc atgccactcc gccggcacaa
    16741 gcgaggcctc cggtgcgccc gcgtaggcca cccagcgacg aacccggtag ccaggccgcg
    16801 cgctacgctc ttgataatag atgttgccca gggagtcggc gccaacgcgc ctgcccttga
    16861 gccaggtgaa cagcctggtg cctaggttca tgccgctcgg tatacggagc tctgccggtc
    16921 cggtccaccg gcgcacttca tccacaggat tttgtggcta atttgcattg tgaccgcaaa
    16981 atgcgctttc cgagtcgcgg cctggcgctt agcatggcgg ccatgaaata tccccggacc
    17041 tggcagggtg tgcgtatgcg gcgtgtgatg gcgcatccgg atccggatgc gccgcaacgt
    17101 caggtcacgc tgcctgccgc ctgggacgat cgcgccgcct ccgcactcgc cgaactggcg
    17161 cccggggggg cgcccgtaat gctggccggc gcagccgagg cctggattgc ccccattgcc
    17221 gagcgtgccc tgcgcgccgg cctggaaacg ccgctcgccg agcgcctgca ccgcatgctg
    17281 ctgttacgcc atggcgcgcc atcactgtcg gtatggcgcg gcaacgccgg cgctgagccc
    17341 ggtttcgtgc tcgatctcgc gtccttccac gacgccgcga tcggtttcga cgcccccgcg
    17401 tttgcagaag cagtcgagac agcggtcact gccctgaccc tgttcgcccc cgccgccacc
    17461 cgtatctctg tgggcatggc cgatctcgcc ggcctgctgg cgctgctggg catcgagtac
    17521 ggctctgatg attcgcttgc gacaacccgc gcggttgccg ccatccttcg cggccgggcc
    17581 gaagctgcat caggcgccat ggcccgtctg ttcggctccg tcgcccctgc cgcgctcgat
    17641 tggcctgccc cgccggcgga aaccccggtg ccgggcctcg ccgaagctgc ccgcgctgcc
    17701 cgcctcgccg ccgccgggtt cgatggcctg cggcacaccg cacttactgc cgtcgccgct
    17761 cccgcagcgg cggatgcact gctcggcgtc gagaccggcg gcattgcccc tgccttctcg
    17821 ccgctggacg agaccggcgc gctcaaccgc accgcacgcg cctggctcgc cgcgcgccat
    17881 atcacgcccg aggccgcact ggcccggttg ctcgccggca acgatccgct gcccgccgcc
    17941 agtgtcaccg gcgcgattgc gatgcacgat gccgtatcgc cgtttgtcca taccatgccg
    18001 gcgcgcccgg aaccgctgcg ccagccgatg cacacagtgc gccgccgcga gcttcccact
    18061 cgccgtgccg gctacacgca aaaggcggcg gtgggcggcc accgcctcta tttacgcacc
    18121 ggcgagtacg acgacggctc gctcggcgag gtgttcatcg ccctgcacaa ggaaggcgcc
    18181 gcttttcgcg gtctgatgga caattttgcc gtcgccatca gcctcggcct gcagcacggc
    18241 gtgccgctgg aagcatttgt cgaggccttc acctttaccc gcttcggtcc agcaggcgcg
    18301 gtcgaaggag atcccgccgt cgcgcacgcg acctcgctgc tcgactacac gttccgtcac
    18361 ctcgccgcga actatctcgg ccgccgcgac atccccgaag cggaaatcga ggaggcggac
    18421 acagtcggca acggcagccg cgaccatgcg cctttgctcc cgctcgatct gcccgcagaa
    18481 tcaccgcgcg cgcgacggcg cggcttcaag gtcgtctcgc gctgaagtag actttgcgct
    18541 gtccaccgcg cccctgccgt cggcgcgcaa ccagaaggag cgtgcgcgat gcccagtcgg
    18601 gtcgaactga aactggccga actcggcatc accctgccca gcccaatgcc gccgatcgcc
    18661 aactacgttc cctatgtcgt caccggggat ctcgtggtgg tatcgggcca ggtgcctgcc
    18721 gtggacggga agatagcgat caccggcaag gtcagttacg gcgtctcgct cgaacagggc
    18781 cgtgaggccg cgcggctttg cttcatcaat ttgctggtgc atctgaaggc ggcctgcggt
    18841 ggcgaccttg accgggtgaa gcgcgtagtg cgcctgggtg gcttcgtcgc ctcgccgccc
    18901 gagttcaggg accacgcgca ggtcatgaac ggcgcctccg atctcgcggt ggcggtattc
    18961 ggcgatgccg gccgccacgc ccgtaccacc atcggagttc ccgcactgcc ggcggacgcg
    19021 gcggtggaag tggaggggat gttcgaaatc agttagactg catcaatgcc cgacggcgcc
    19081 gccacgctca cactgacact gcaccagaag atcgccgacg tccccgccgc cgactgggat
    19141 gcgtgcgccg gtccgggcaa ccctttcgtc agccacgcct ttctgagcgc gatggaggat
    19201 agcggttccg ccagcgcccg caccggctgg ctgccacagc acgcggtgct gcgcggcgag
    19261 gcgggacagg tggtcgccgt cgccccgatg tacgccaaaa gccacagcta cggcgaatat
    19321 gtgttcgatc acggctgggc gcacgcgctg gaacgtgccg gcggcgatta ctatccgaag
    19381 cttcaggtgg cggcgccgtt cagtccggtg cccgggccgc ggctgctgcg ccggcccgac
    19441 tccggcatcc cagtggccgc cctggcttcg gcgctggaac aggcctgcgc atcgctcggc
    19501 gtttcctcgg tgcacatcac cttctgtgcg gaagacgaat ggcagcagct tggcgatgcc
    19561 ggctggctgc agcgcgtcgg catgcagttc cactgggaga atgccggcta cgcaacgttc
    19621 gacgatttcc tcgggtcgct gtcatcccgc aagcgcaagg tgctgcgacg cgagcgccgt
    19681 gatgcgaatg cggccggcct gacattccat gctctgagcg gtgccgacat caaggaagcg
    19741 cactgggacg cattctatcg cttctaccgt tcgacggtcg atcgcaaatg gggctccgca
    19801 tatctgtcgc ggcagttctt ctcgctgctc ggcgagcgcc tcggtgaccg cgtcgtgctg
    19861 atgctggcgg acaatgccgg caagccggtg gcgggcgctc tgaacctcgc aggtgacgag
    19921 gcgctgtatg gccgcaactg gggctgccgc ggcgactggc cgttcctgca tttcgaactg
    19981 tgctactacc gcgccataga ctgggcgatt gagcacaaac tgcctcgcgt ggaggccggc
    20041 gcgcaggggc ggcacaagat ccagcgcggc tatctgccga agccgacata ttccgcgcat
    20101 tggatcgcgc acaccggttt gcgtcgggca attgccaact tcctcgagga tgagcgcctt
    20161 ggcatccatg ccgagatggc gcatctggcg gagcagtcgc cgtttcggaa ggaagatgag
    20221 acggactgaa agacccagtg tcattgcgag cgaagcgaag caatctcgcc aaaattgcgt
    20281 tgcccgtcgc acaagcccgc caaaaatgag cgttcatccc tcccgatgct ttccgcccat
    20341 gtcgtagcga tcgtcctgct ggctgcaacg ctgcacgccg gatggaacgt cctggtccgc
    20401 tccggccgcg atcgtcgcca ggaaaccgcg ctgctgctca tcggcggcac attctgggcg
    20461 gtcgtgctgc tgccgttcct gcccggtctg ccgctggcaa gctggcccta tcttctgatc
    20521 tcctctgtgc tcaacggcgt attcttcatc ttgatcgccg aggcctattc gcgcggcggc
    20581 atcgcgctgg cctatccggt gatgcgcggc gtggctccta tgctgacact ggttgccgcc
    20641 tggcttgtgc ttcacgaggt attgcccacc gccggctggt tcggtgttgc cgctatctgt
    20701 ggcggcgtcg tgctgctggc gcgcggtcgc ggtgaccccg aggaagggag ggcggtccgc
    20761 tttgccctcg ccaacgccgt agtgatatcc gcctatacgc tgaacgatgc gatcggtgtg
    20821 cgcatcgcgc aggaaccact gtcgtacacg ctgtggatgt tcccgctgtc ggccgtgccg
    20881 acatggctgt ggctgcgcca ccgcacttca ttgcactggc cggcgccgcg agaggcgctg
    20941 cgcgggatcg gcggcgccgg cgccagcatc ggtgcctatg cgctggtgct gtgggcgatc
    21001 acgcaggctc ctgtcgcccc ggtggccgcc ttgcgtgagt ccagcattct catcggcgtg
    21061 gtcctggcgc gcgtgctgct gggcgagcag cccggccgcc gcggttgggg cggcgcggtt
    21121 gcgatcgcgg tgggagcggc gatactgcgg ctggcgccgg gatagggcgc actatccgaa
    21181 cctacgcggc gataaacgcc actccgtcct cctcgaacac cgcacaggtc agccgcccgc
    21241 cacacccggc cccagtgtcg atcccgatcc ggttctcctt catcaccggt gttggcgccg
    21301 ccgagtgccc gtgcaccacg accgcgccga aatcctgctc gctggccagg aagctctgcc
    21361 ggatccccag cagatcgtct tccgcctgct tttccagagc cacgcccggc cgtacccctg
    21421 cgtgcacgaa aagatacccg cctgcccgat acgccggctc caatccctgc atgaacgcga
    21481 catgcgcctc tggtacgaac ctcacccaat cagtccgacg cacctcgacc ccaatccccc
    21541 agcttcgtag cgctgcctca ccgccggtat gcagccagtc cgtcgcggcc gccccatcgc
    21601 ccgacagcgc gtcgagcgcc gtccgctcat gatcccccat caggttcacc gtccgcagcc
    21661 ccgccaccgg cgggcccgac gccagcagct cgatcactcc cgcactatca ggcccgaagt
    21721 cgatatagtc cccaagatgc acgagcaccg ccgataccgg ctggtttcgc ttgaaatcat
    21781 ctgccaccgc cgcatgcaga gcctgcaact cgtccagaca tccgtgtata tcgccaatcg
    21841 catacacccg atgcccgcgc accagccgcg ccggcgccag cacgaatcgc aacgacgccg
    21901 catcatgcgc gacacgcggc ggactccagg tcggcacggc ggtcgcatcc aggtcggcac
    21961 gaagtcgagg ccgcgaagcg cggcgaacgg ggcgtcgcat accgcgcact atgccgtgcg
    22021 ccgttgtgcc ccggcaatgc ggcagggtag gctccgatcc tgacgagtaa gcaccggagg
    22081 aaacctttgg cgaagatcac cttccccgtc gaggctgggc acatcatgct gtttgcccgc
    22141 gctgtcggcg actacaaccc cgtctaccac gatgccgaag ccgcaaagaa aagcgaagtc
    22201 ggcggcatca tcgcgccacc gacatttccc caggcgatgg cgcagttcga tcccgactat
    22261 ccgctgcgct ggcgcccagg gcagccctgg ttcggctctg ggaagacccc cagtggtgtg
    22321 accgagcgag cctccggcgg cggcggcctg cacgccgagc agcacttcga atatcatcgc
    22381 ccgctgcgcc ccggcgacgt gctgacggtg gagaccaagc ccggcaacac ctgggagcgg
    22441 gaaagcaagc gcgccggcaa gctgaccttc cgcgaacgca tcgcggaata tcgcgaccag
    22501 aaaggcgaac ttgtcatcac cgcgcgcagc gtcggcgtga cgacagaacg ccccgtcgag
    22561 cggagctgat cccatgcctc tgaaagccag taaactgaaa gtcggcgaca cgcacagcga
    22621 gcgcgtcgcc gagaacctct cgcgcaccca gatcgtcatg tattgcggcg
//
LOCUS       DATGEM010000398         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_244434, whole genome shotgun sequence.
ACCESSION   DATGEM010000398 DATGEM010000000
VERSION     DATGEM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244434"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VKQ27_12750"
     CDS             <1..749
                     /locus_tag="VKQ27_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HLY89842.1"
                     /translation="TDDGKTLLGHARHLLEGAEEMEAALGRQSGEPRGLVRLGTSVSG
                     GLFLATRLPVLLARYPGLSVELVMSDRHTDMIEERLDLAFRSGEIADASLVARRCGVL
                     GRAVVAAPIYLERQGAPERPEDLADHACLIHDATADPDVWRFTGPEGPVSVQVSGAFL
                     ANDSEAVRRAARAGHGVALLPEIQLIDDLRAGRLFRLLEDYPSQRVPVHIVYPSRRNL
                     APRTRVVMDFLVEQTRQISALLQQGAEVLT"
     gene            816..2405
                     /gene="guaA"
                     /locus_tag="VKQ27_12755"
     CDS             816..2405
                     /gene="guaA"
                     /locus_tag="VKQ27_12755"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HLY89843.1"
                     /translation="MPDSLPDPATTEALAGERVLILDFGSQVTQLIARRLRESGVYCE
                     IWPFNTEPGRIADWRPRGIILSGGPASVHEAAAPLAPQAVFSMGVPILGICYGEQLLC
                     QQLGGEVLGSDHREFGRAFIEVTEECALFQGVWPKGAREQVWMSHGDRVTRLPPGFRV
                     VGVSEGAPFAAIADDRRQFYGVQFHPEVVHTPHGAALLRNFTHRVLGLSGSWTMAGFR
                     ATEIARIRAQVGRGRVICGLSGGVDSSVAAVLIHEAIGDQLTCIFVDHGLLRQGEAQE
                     VVTTFRDRFNIRLVHCDASEEFLSALEGVTDPEVKRKTIGRLFIDVFDDEARKLGGAD
                     WLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMRMKLVEPLRELFKDEVRALG
                     RELGIPEAIVGRHPFPGPGLAIRVPGEITREKLALLRRVDAIFLEEIRAAGLYDAIWQ
                     AFAVLLPVRTVGVMGDGRTYDHACALRAVTSTDGMTADVYPFDAGFLTRVAGRIVNEV
                     RGINRVTYDITSKPPGTIEWE"
     gene            2700..>3003
                     /locus_tag="VKQ27_12760"
     CDS             2700..>3003
                     /locus_tag="VKQ27_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HLY89844.1"
                     /translation="MLTDAALKHLKPKEKIYKVTDRDGMYVLVKPSGTLSFRLDYRMN
                     GRRETVTFGKYGPAGLSLARARELCIDAKRAISEGRSPAIEKQREKRRLLEAKSFGE"
BASE COUNT          521 a          804 c         1116 g          562 t
ORIGIN      
        1 tgaccgatga tggcaagacg ctgctggggc atgcgcggca tctgctggaa ggggccgagg
       61 aaatggaggc ggcgctgggg cggcagagcg gcgagccgcg aggattggtg cggcttggga
      121 cctcggtttc cggtgggcta ttcctggcga ctcggctccc ggtgctgctg gcgcgatatc
      181 cagggctttc ggtcgagctg gtgatgagtg atcggcacac cgacatgatc gaggagcgcc
      241 tggacctggc gttccgcagc ggcgaaatcg ccgatgcctc gctggtggcg cggcgatgcg
      301 gggtgctggg tcgcgcagtg gtggcggcgc caatctacct ggagcggcag ggtgcaccgg
      361 aacggccaga ggacctggca gatcacgcct gcctcatcca tgacgcaacg gcggatcccg
      421 atgtctggcg atttaccggg ccggaggggc cggtgagcgt gcaagtgtca ggcgcgttcc
      481 tggcgaacga cagcgaggcg gtgcgacgag cggcgcgcgc ggggcacggt gtcgcgctgc
      541 tgcccgagat acagttgatc gacgatctgc gggcggggcg gctgttccgg ctgctggagg
      601 attatccgtc gcagcgggtc ccagtgcaca tcgtatatcc gtcgcggcgg aacctggcgc
      661 cgaggacgcg ggtagtgatg gatttcctgg tggagcagac ccggcagatt tcggctctgc
      721 tgcagcaggg ggcggaggtg ctgacgtagg cacggcgcac ttgccagcgg cggttcggcg
      781 tggtccaagc gcgaggatca tgcgcggagg cccttatgcc cgattccctg cctgatccag
      841 ccaccaccga agcgctcgcc ggcgaacgcg tactgatcct ggacttcggc agccaggtta
      901 cgcagttgat cgcccgcagg ctgcgcgagt cgggcgttta ttgcgagatt tggccgttca
      961 atacagagcc ggggcggatc gctgactggc gaccccgagg gatcatcttg tcgggcgggc
     1021 cggcttcggt gcacgaggcg gcggctcctc tggcaccgca ggccgtgttc tcgatgggcg
     1081 taccgatcct gggtatctgc tacggcgaac agcttctctg ccagcagctt ggcggtgagg
     1141 tgctggggtc cgatcatcgc gagttcgggc gcgccttcat cgaagtgacg gaagaatgcg
     1201 cgctgttcca gggggtgtgg ccaaagggcg cgcgcgagca ggtctggatg agccatggcg
     1261 atcgggtgac acggctgccg ccggggttcc gcgtcgtggg ggtgagcgaa ggggcgccgt
     1321 tcgccgcgat cgccgatgac aggcggcagt tctacggcgt gcagttccat cccgaagttg
     1381 tccatacgcc gcacggggcg gctttgctgc gcaatttcac acatagagta ctggggttgt
     1441 ccgggtcctg gacgatggca gggttccggg cgacggagat cgctcggatc cgggcgcagg
     1501 tcggccgtgg gcgagtgatc tgcggactgt cgggcggggt ggattcgtcc gtcgccgcgg
     1561 tgctgatcca tgaggcgatc ggcgaccagt tgacctgcat cttcgtggac catggcttgc
     1621 tgcggcaggg cgaggcgcag gaggtggtca ccacgttccg cgaccggttc aacatccggc
     1681 tggtgcattg tgatgcgtcg gaggagttct tgagcgcact ggagggcgtg accgatccag
     1741 aagtaaagcg caagacgatc gggcggctgt tcatcgatgt gttcgacgat gaggcacgta
     1801 agctgggcgg tgcagattgg ctggcgcagg ggacgctgta tccggatgta atcgagagcg
     1861 tgagctttac cggtgggcct tcggtgacga tcaagtcgca ccataatgtg ggtgggctgc
     1921 cggagcggat gcggatgaaa ctggtggagc cgctgcggga gctgttcaag gatgaggtgc
     1981 gcgcgctggg gcgagagctg gggatccccg aggcgatcgt cgggcggcat ccgtttcccg
     2041 ggccagggct ggcgatccgc gtgccagggg agattacgcg cgagaagctg gccctgctgc
     2101 ggcgcgtaga tgcgatcttc ctggaggaga tccgcgcggc gggattgtat gacgcgatct
     2161 ggcaggcgtt tgctgtgctg ctgccggtgc ggacggtggg ggtaatgggg gatggacgga
     2221 cctatgacca cgcctgcgcg ctgcgcgcgg tgacgagcac ggatgggatg acggcggatg
     2281 tatatccatt cgatgcgggg tttctgacgc gggtggctgg tcggattgtc aacgaggtgc
     2341 gcgggatcaa ccgggtgacc tacgacatta ccagcaagcc gccggggacg atcgagtggg
     2401 aatgattttg gcccatccgg ggctatccaa caacgtcccg ggaacctcaa taacacactg
     2461 ataaacaaaa taaaatcatc caaagacgtt cggggaccat cggccggtag agatcgccgc
     2521 tgacggactc cttgacggtg cgctctccca ttgcgaaatc cgatttcgtc cgataccgtc
     2581 agggtatgag caccctctga cggtcttttt ttcgctttaa ctagctgtaa gaaaaagaga
     2641 ttttggcgtg aggatgcccg aaaacggcct gacggtcttt tttggaggag gcagtcccga
     2701 tgttgaccga tgcagccctt aagcatctga aaccaaaaga gaaaatttac aaggtgacgg
     2761 accgggacgg catgtatgtc cttgtaaagc cgtcgggcac cctctctttt cggctggatt
     2821 accggatgaa tggacggcgc gagaccgtca cgttcggcaa gtacggcccg gccgggctgt
     2881 cactcgctcg tgcgcgcgag ctgtgcatcg acgcgaagcg ggcgatcagt gagggacgat
     2941 cgccggcaat cgagaaacag cgtgagaagc gccggctctt ggaagcgaag agctttggcg
     3001 agt
//
LOCUS       DATGEM010000399         8636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_899098, whole genome shotgun sequence.
ACCESSION   DATGEM010000399 DATGEM010000000
VERSION     DATGEM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899098"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1333
                     /gene="lysA"
                     /locus_tag="VKQ27_12765"
     CDS             2..1333
                     /gene="lysA"
                     /locus_tag="VKQ27_12765"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLY89845.1"
                     /translation="MATVTMSTTSEPTAAELIAGRPHLSMHAMDGLVMDGVPLNLVAD
                     RVGTPVWVYSAVAMRRRYRMLADALAQAGLDADIHYAMKANDRLAVLRLFSREGAGAD
                     VVSEGELRRAREAGIPASRIVYSGVGKSERELRLAVAEDIVQINVESAEELQMLSGIA
                     AAAGRLARVALRVNPDVDAGTHAKITTGLAQNKFGVAFDEAIALYRHAAGLPGIEPVG
                     LAVHIGSQVLSLAPYRAAYGRLAEMVRGLREQGLTVRSVDCGGGLGIPYRDEPAPLPG
                     ALAGAIRGAFHNLDVRIAIEPGRWLVGPAGVLLSSVILVKQAGDSRFVVLDAAMNDLI
                     RPAMYDAWHGIVAVSAVDAVAPTEPADVVGPVCETGDTFARQRLLPPLAPGARVAILD
                     AGAYGAVMSSAYNSRPPAAEVMVDGDSWSVIRARPSHADLWQGESMPHWLQ"
     gene            1330..3900
                     /locus_tag="VKQ27_12770"
     CDS             1330..3900
                     /locus_tag="VKQ27_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HLY89846.1"
                     /translation="MKAPGGDFDGLLRRLAGRRVLARAVLLFERIWPAIWPPLGVAGV
                     FVCAALLDLPRLLPPWVHIAVLALTALLIIGLLYRGLRGIAAPDDRAADRRLEIASGL
                     THRPLAVLTDRPAQGRVEPDEASVAIWQAHVFRAIRQVRRLRVGLPRPGLARRDRFAL
                     RAALIVALIASFSIAGADAPGRLAQAMEPTLPRDTPAPATELQAWITPPAYTRLAPIF
                     LKADGGTVSVPTGSQLTVSITGGDGTPSLSLNGNPEPFRQLDKVSYQAQRALSEGGRL
                     LVRRNGGELASWDLTVVDDQPPVAEWSEQPGRAGNSQQTRLPWRVSDDYGVVSLQAEL
                     RLRDRRGAPPVIISVPLPGGAPKGAHGINQQDLTANPWAGLPVVAQLVARDALNQPGR
                     SKEAGFILPERPFENPVARALMAIRRGLSLHPDDRDSAVGGLDALLMKPEALGDDLGA
                     YLNLGGIYYLLERDKEPDAVGNAQQRMWELALHLEEGRTERTARALDQARQAAKDALD
                     KATRDPTPANRDELDKKLKELEQAIEQHLQALMDEARRNNDEMPFDPDAQHLSNRDLD
                     RLADQAREAAREGRMQDAQQQMAELERMLDRLRNARAEHGRDGQRNEAQRRRGRQQMG
                     VVQDMIGRQGNLLDHDQGRIGDTNPFRGNQAPTMQQDQASEREADGRVQQALRRALGE
                     LMQQFSDLTGKLPPSLGEADTAMRDSLRQLGQGDDQAAGASQQKAIEALQKGGREMGQ
                     AMAQQFGTPNGGDGSEPDGEQDGMMGLGLQDGRGDDRGDGWRRGPPPGRADSGRDPLG
                     RHYGQGTSGADESGDVVVPEERERQRTQAVEEELRRRGSDLTRPQQELDYINRLLKQF
                     "
     gene            3907..5136
                     /locus_tag="VKQ27_12775"
     CDS             3907..5136
                     /locus_tag="VKQ27_12775"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF028064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin C-terminus-like muramidase"
                     /protein_id="HLY89847.1"
                     /translation="MGRGPQPGAKPPPNPADDGWAAAFAAANAGATILDDPEELEDRA
                     IFRWMKGHVLPRFSHVLPHVKGHQFILGNTPLPYHWMEQCVRFATLVEAREFVDHLRR
                     NTAAMHIVVDLLHKVDGSLLICETVDASLDRLARMLMSGRIWVMPFQRPQQPDIGVTD
                     PGVYAGLNGQFGTKLDGAKLSAWEGGQYLRGYVPFTREKNAAGVVETVVAGRSGMTIA
                     TGFDIGQHTAQYLQGVTGLSARSLALLRPYAGQQFTHLGKDAVIRRIGTLGPIPLIDK
                     GEADALDRAVAEDTLRATMTAWAANRKSTVPVFGMMPSAWQTVMFSRTYNQGPGWVLP
                     GSPTRAFFTFATEGKWREAATALRDAPVTAAWYRSRLRSEAGYLATDMPRPLVPTTPA
                     PSPSAKPGALPSVPARP"
     gene            5148..5891
                     /locus_tag="VKQ27_12780"
     CDS             5148..5891
                     /locus_tag="VKQ27_12780"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HLY89848.1"
                     /translation="MLALAALPAKAAGIDCAKARTATERAVCGDAELRKLDASVASDF
                     ADALAATQGATRQALLAQQRRWLSQRDHGCKGDDAACLRASYQARYDALETLTARVSP
                     GNPTLSDVTAVALLGSWKIGDDVVPGAPDQRVPATELPAFLPKPGSTITGQPGKLCFG
                     NGECLPFGLDQQALGMAAGTEALHLPPSTPYYVAYLSGKSAYGLIPRQDGSLLAQFQA
                     CDKTYTSCTNAFQVWTPASPEAGVRVLAH"
     gene            6051..8081
                     /gene="tssI"
                     /locus_tag="VKQ27_12785"
     CDS             6051..8081
                     /gene="tssI"
                     /locus_tag="VKQ27_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HLY89849.1"
                     /translation="MPTWSRDTALLTMTSPLGADALIPYEFSAREAISAPFLFEISAF
                     SQQGIIDPDSLLYQPVCVKVQQQAGAGVVRYFHGIVQSLSAEGGLTQIRGGSDLHAYR
                     ITVVPKLWFLSQTVDCRVFQELTTVGILTQMFNDMGVSDVTYPPAGAEREYTVQFNES
                     DLHFATRLMEEEGYYYFFQHTDSAHKLIIANANTSFQDVPDAASMHFGGGASQGGRIF
                     DWQRTAATTRGKMTFKDYDPTKSTTLLQNDQPTTLKTGGAASRDDFRWPAVSFDSGTI
                     TNRTKHEMEAAEAAVSIYGGASKFAPLYAGGKFTLGNKPASSYDAAYVLHSVTHHATD
                     DTFLNSGGTPSYENHFTCFPSSVTWRQPLVTPRPRMDGIHTAVVIGPSGEEIYTDDLA
                     RVKVRFFWDHRSEATDAQDVWARVIMPWAGNGWGAQFIPRIGTEVAVAFVDGDPDRPI
                     VVGGLYNDQMTPIYSSSDKTKSGFRTRSSLKGGTSDFNEYTFDDKKGSELIYEQAQKD
                     LSTYVKNDQTIKIDHDRMVTVKNDETVKIQNNQTITVTQDHALTVSQGNHTITVSTGN
                     HSTTISKGNHSTTVSMGNMDSTVSMGNLSTDVKMGNISTKADLGQISNEAMQSIELKV
                     GANSIKIDQMGVTITGMMIKIQGQVQTQVEGMMTQISANAMLQLSGPITMIG"
     gene            8083..>8636
                     /locus_tag="VKQ27_12790"
     CDS             8083..>8636
                     /locus_tag="VKQ27_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89850.1"
                     /translation="MSPTVSKLAAIDLASLTPHVGLSPEASAATAGSGKVEDALAKLE
                     ASGFLMEATRLMAHALPKREAVWWSCMCSSHTAPPDLPEPDQNCRRAAEEWVRGQNEK
                     LRYAAWDTAQASGYGTPEVYTAVAAFWSGGSMSPEGQPAVPPQPHFTGNAVAGVIALS
                     AVRGDPTKRDARLKRFLESGRNIAA"
BASE COUNT         1549 a         2679 c         3042 g         1366 t
ORIGIN      
        1 catggcgacc gtaaccatgt ccaccacgtc agagccgacc gccgccgaac tgattgcggg
       61 ccggccgcat ttgtccatgc acgcgatgga tggccttgtc atggacggcg tgccgctcaa
      121 tctggtcgcc gatcgcgtcg gcacgccggt atgggtctat tccgcggttg ccatgcgccg
      181 gcgctaccgg atgcttgccg atgccctcgc gcaggcaggg ctggacgcgg acatccacta
      241 tgcgatgaag gcgaacgacc ggctggcggt gctgcggttg ttcagtcggg agggtgccgg
      301 cgccgacgtg gtgagcgaag gcgagctgcg gcgggcccgt gaggccggca tcccggcaag
      361 ccgcatcgtc tactccggcg tgggcaagag cgagcgcgag ctgcggctgg cggtggccga
      421 ggacatcgtg cagatcaacg tggagagtgc cgaagagctg caaatgctgt ccgggattgc
      481 ggcggcggcg ggccggctgg cgcgcgtggc cttgcgggtg aacccggacg tcgatgcggg
      541 cacgcatgcc aagatcacca ccggcctggc gcagaacaag ttcggcgtcg cgtttgacga
      601 ggcgattgcg ctttaccggc acgcggcggg tttaccgggg atcgaacccg ttggactggc
      661 ggtgcatatc ggcagccagg tcctgtcgct cgcaccgtat cgcgccgcgt atggacggct
      721 ggccgaaatg gtgcgtgggc tgcgggagca ggggctgacg gtccgcagcg tcgattgcgg
      781 cggcgggctg ggtattccgt atcgcgacga gccggcgccg ttgcccggag cgttggccgg
      841 ggctatccgc ggcgccttcc ataacctgga tgtccgcatc gcgatcgagc ccgggcgctg
      901 gctggttggc cccgccggcg tattgctcag ctccgtgata ctggtcaagc aagctggcga
      961 cagccgcttc gtggtgctcg acgcagcgat gaacgacctg atcaggccgg ccatgtacga
     1021 tgcgtggcac ggcatcgtgg cggtttcggc ggtcgacgcc gtggcgccga cggagcctgc
     1081 cgacgtggtg ggaccggtgt gcgagaccgg cgatacgttt gcccgacagc gcctgctgcc
     1141 gccacttgca ccgggcgcgc gcgtggcgat cctcgatgcc ggggcgtatg gcgccgtgat
     1201 gagctcggcc tacaactcgc ggccgcccgc cgccgaggtg atggtggacg gcgacagctg
     1261 gtcggtcatc cgcgcgcggc caagccatgc ggacttgtgg cagggcgaga gcatgccaca
     1321 ttggctgcaa tgaaggcacc gggcggagat ttcgacggcc tgctgcgccg cctggccggc
     1381 cggcgcgttc tcgcccgcgc ggttctgctg tttgagcgga tctggccggc aatatggccg
     1441 ccgcttgggg ttgccggcgt gttcgtctgc gcggcgctgc tcgacctgcc gcgcctgttg
     1501 cccccgtggg tgcatatcgc cgtactcgca ttgacggcgc tgctgatcat cggattgctg
     1561 tatcgcggcc tgcgcggcat tgccgcaccg gatgacaggg cggccgatcg ccggctcgag
     1621 atcgcctcgg gcctgaccca tcgaccgctc gcggtactga ccgataggcc ggctcaaggc
     1681 cgggtggagc cggacgaggc gtccgttgcg atctggcagg cgcatgtatt ccgcgccatt
     1741 cgccaggtgc ggcggttgcg cgtcgggctg ccgcgacccg gcctggctcg ccgagatcgc
     1801 ttcgcgttgc gggcagcatt gatcgttgcg ctgattgcat cattctcgat cgcgggggcg
     1861 gatgccccgg ggcgcctggc gcaggcgatg gaaccgacct tgccgcgcga cacgccggcg
     1921 cccgcgaccg aattgcaggc ctggatcacg ccgcccgcct acacgcggct cgcaccgatc
     1981 tttctcaagg cggatggcgg cacggtgtcg gttccgaccg gttcgcaact gaccgtcagc
     2041 atcacggggg gcgatggcac ccccagcctg tcgctcaacg gcaatcccga gccattccgc
     2101 cagctcgaca aggtgagcta tcaggcgcag cgcgcgctga gcgagggcgg gcggctgctg
     2161 gtgcgccgga atggcggcga actggcgagc tgggacctta cggtggtgga tgatcagccg
     2221 ccggtggccg aatggagcga acagccgggg cgggccggca acagccagca aacgcggttg
     2281 ccctggcgcg tgtccgacga ttatggcgtt gtctcgctgc aggccgagct gcggttgcgt
     2341 gatcggcgtg gtgcgccgcc cgtcatcatc agtgttccgc tgccgggcgg cgcccccaaa
     2401 ggcgctcacg gcatcaacca gcaagacctg acagcaaatc cgtgggctgg gctccctgtc
     2461 gtcgcccagc ttgtggcgcg agatgcgttg aaccagcccg ggagaagcaa ggaggcggga
     2521 ttcatcctgc cggagcggcc gttcgagaac ccggtcgcac gcgcgctgat ggcgatccgg
     2581 cgcggcctca gcctgcatcc ggatgatcgc gattccgcag tgggcggcct cgatgcgctg
     2641 ctgatgaagc cggaggcgct cggtgacgat ttgggcgcct acctcaatct cggcgggatc
     2701 tactatctgc tggagcgtga caaagagccg gacgccgttg gcaatgcgca gcagcggatg
     2761 tgggagcttg cgctgcacct cgaggaaggc aggaccgaac gcaccgcgcg cgcactggat
     2821 caggcgcggc aggcagccaa ggatgcactg gacaaggcga cccgcgaccc caccccggcg
     2881 aatcgggacg agctcgacaa gaagctgaag gagctcgagc aggcaatcga gcagcacctg
     2941 caggcgctga tggacgaggc gcggcgcaac aatgacgaga tgccattcga ccctgatgca
     3001 cagcacctgt ccaatcgcga tctcgatcgg ctggcggacc aggcgcgcga ggcggcccgc
     3061 gagggccgga tgcaggatgc gcagcagcag atggcagagc tcgaacgcat gctcgatcgt
     3121 ctgcgcaacg cccgcgcgga gcatggccgc gatggccagc ggaacgaggc gcaacgccgc
     3181 cgtgggcgcc agcagatggg cgtggtccag gacatgatcg gccggcaagg aaatctgctc
     3241 gaccacgacc agggtcgcat cggcgacacg aacccgttcc gtggcaatca ggcgccgacg
     3301 atgcagcagg accaggcttc ggagcgcgag gccgacggcc gggtacagca ggctctgcgc
     3361 cgcgccctcg gtgaactgat gcagcagttc agcgatctga ccggcaagct gccacccagt
     3421 ctcggcgaag ctgatacggc gatgcgcgat tcgctgcgcc agcttggcca gggggacgac
     3481 caggcagcgg gggcgtcgca gcagaaggcg atcgaggcgt tgcaaaaagg cgggcgcgag
     3541 atgggccagg cgatggcgca gcagttcggc acgccaaacg gtggtgacgg cagtgagccg
     3601 gacggagagc aggacgggat gatgggtctg ggcttgcagg acggccgtgg cgacgatcgc
     3661 ggtgacggat ggcggcgggg cccgcccccg ggacgcgctg attcgggacg cgatccgctt
     3721 gggcggcatt atggacaagg cacatctggc gccgacgaga gcggcgacgt ggtggttccc
     3781 gaggagcgcg agcggcagcg aacccaggcg gtggaggagg aactgcgccg ccgcggctcg
     3841 gatctgacgc ggccgcagca agagctggac tacatcaacc ggctgctgaa gcagttctga
     3901 tcgccgatgg gacgaggacc gcagccaggg gcgaagccgc cgccaaatcc ggctgatgac
     3961 ggctgggcgg ccgcgtttgc ggcggcgaat gccggtgcca ccattctgga tgacccggaa
     4021 gagctggagg atcgcgcgat cttccgctgg atgaaggggc acgtcctgcc gcgattcagt
     4081 cacgtgctgc cgcacgtcaa aggccaccag ttcatcctgg gcaacacgcc cctgccctat
     4141 cactggatgg aacaatgcgt tcggttcgcg acgctggtgg aggcgcgcga gttcgtcgat
     4201 catcttcgcc gcaacacggc ggcaatgcac atcgtcgtcg acctgctgca caaggtggac
     4261 gggtcgctgc tgatctgtga gaccgtcgat gcgtcgctgg atcggctggc gcgcatgctg
     4321 atgagtggcc ggatctgggt gatgccgttc cagcgtccgc agcagccgga tatcggcgtc
     4381 acggatccgg gcgtttacgc cgggctgaac gggcagttcg gcacaaagct ggatggcgcg
     4441 aagctctcgg cgtgggaggg cggacagtat ctgcgcgggt atgtgccgtt cacgcgtgag
     4501 aagaacgcgg caggcgtggt tgagacggtg gtggccggac gcagcggcat gacgatcgcg
     4561 acgggcttcg acatcggcca gcacaccgcg caatatttgc agggcgttac cgggctgtcg
     4621 gcgcgctcgc ttgcgctgct acggccctat gcggggcagc agttcaccca tcttggcaag
     4681 gacgcggtga tcaggcggat cggtacgctc gggccgatcc cgctgatcga caagggcgag
     4741 gccgacgcgc tggaccgcgc ggtcgccgag gacacgctga gggcgacgat gaccgcctgg
     4801 gcggccaatc ggaaatccac cgtcccggtg ttcggcatga tgccatcggc atggcagacc
     4861 gtgatgttct cgcggaccta caatcagggg ccgggctggg tgctgcccgg cagcccaacg
     4921 cgggcgttct tcaccttcgc gacggaaggg aagtggcgcg aggcggcgac cgcattgcgc
     4981 gatgctccgg tgacggcggc gtggtaccgc tctcgcctga ggtcggaggc gggctatctg
     5041 gcgaccgata tgccgaggcc actggtgccg acgacgccgg cgccatcacc ttccgccaag
     5101 ccaggtgcgc tgccatccgt gcccgcgcgt ccctgactgt cgcgctgttg ctggcgctgg
     5161 cagcgctgcc agccaaggcg gccggcattg actgcgccaa ggcgcggacc gcgaccgagc
     5221 gcgctgtctg tggggacgcg gagctgcgga agctcgatgc gtcggtcgca tccgacttcg
     5281 ctgacgcgct tgctgcgacg cagggggcaa cgcgacaggc gttgctggcg cagcagcgcc
     5341 gctggttgag ccaacgcgac cacggttgca agggcgatga cgcggcctgt ctgcgagcga
     5401 gttaccaggc gaggtacgat gcgctggaga cgctgacggc gcgggtctcc cccgggaatc
     5461 cgacgctttc agatgtgact gccgttgcgt tgctgggcag ctggaagatc ggagacgacg
     5521 tggtgccagg ggcgcccgac cagcgggttc ctgcgacgga actgcctgcc tttctgccaa
     5581 agccgggatc cacgatcact ggtcagcccg gcaagctgtg tttcgggaat ggcgagtgcc
     5641 tgcccttcgg actggaccag caggcgctcg gaatggcggc ggggaccgaa gcgctgcatc
     5701 tgccgccatc cacgccgtat tacgtcgctt atctgtcggg caagtcggca tacggactga
     5761 tcccgcgtca ggatggcagc ctgcttgccc agttccaggc ctgcgacaag acgtacacgt
     5821 cgtgcaccaa cgcgttccag gtgtggacgc cggcgagtcc ggaggccggc gtgcgtgtat
     5881 tggcacactg agcggggagc ggcggaattc tccctgccag aaattcccct ccacaaacgg
     5941 cacgttctgc tattctaccg atgcggtccg gtgcggggtg ggcgactgcg acaatcgaat
     6001 gctacgagca agcagcgtta acgtcctgaa caacggatag accaaaagac atgccaacct
     6061 ggtcccgcga caccgccctt ctgacgatga cgtcaccgct cggggcggac gcacttatcc
     6121 cgtacgaatt ctcggcgcgc gaggcgatca gcgccccttt cctgttcgag atcagcgcct
     6181 tcagccagca gggcatcatt gatccggaca gcctgctgta ccagccggtt tgcgtgaaag
     6241 tgcagcagca ggccggcgct ggtgtcgtgc ggtatttcca tggcatcgta cagagcctca
     6301 gtgccgaagg aggattgacg cagatccgcg gcgggagcga cctgcacgcc taccgcatca
     6361 cagtggtgcc gaaactgtgg ttcctgagcc agacagtcga ttgccgggta ttccaggagt
     6421 tgaccaccgt cggcatcctg acccagatgt tcaacgacat gggcgtgtcg gacgtcacct
     6481 atccgccggc cggcgcggaa cgcgaatata cggtgcagtt caatgaatcg gacctgcatt
     6541 tcgcgacgcg actgatggag gaagaaggct actactattt cttccagcac accgacagcg
     6601 cccacaagct gatcatcgcc aacgccaata cgtccttcca ggacgtgccg gacgctgcca
     6661 gcatgcattt cggcggcggc gccagccagg gcggccggat cttcgactgg cagcgcaccg
     6721 ccgccacgac gcgcggcaag atgacattca aggattacga tccgacaaaa tcgacaacgt
     6781 tgctgcagaa cgaccagccg acgacgttga agaccggcgg cgcggcgtca cgcgacgatt
     6841 ttcgctggcc ggcggtgtcg ttcgactccg gcaccatcac caaccgcacc aagcacgaga
     6901 tggaggcggc cgaggcggcg gtttccatat acggcggcgc ctcgaaattc gcgccgctct
     6961 atgccggtgg caagttcacc ctcggcaaca agccagccag ctcgtacgac gctgcctatg
     7021 tgctgcacag cgtcacgcac catgcgacgg acgacacgtt cctgaattcc ggcgggacgc
     7081 cgagctacga aaaccacttc acctgcttcc cgtcgtcggt gacgtggcgg cagccattgg
     7141 tgacgccgcg gccgcgcatg gacggcatcc acaccgctgt tgtcatcggc ccttcgggag
     7201 aagagatcta tacggacgat ctggcgcgtg tgaaggtgcg gttcttctgg gatcaccgct
     7261 cggaggcgac cgatgcgcag gatgtctggg cacgcgtcat catgccgtgg gccggcaacg
     7321 gctggggcgc gcagttcatc ccgcgcatcg gcacggaagt ggcggtggca tttgtcgatg
     7381 gcgatcccga caggccgatc gttgtgggtg gcctgtataa cgaccagatg acgccgatat
     7441 actcgagcag cgacaagacg aagtcaggct tccgcacgcg gtcgagcctg aagggcggga
     7501 catcggactt caatgaatat acgttcgacg acaagaaggg cagcgagctc atttacgagc
     7561 aggcgcagaa ggatctgtcc acctatgtga agaacgacca gacgataaag atcgatcacg
     7621 accgcatggt gacggtgaag aacgacgaga ccgtcaagat ccagaacaac cagacgatca
     7681 ccgtgacgca ggatcacgcg ctgacggtgt cgcagggcaa ccacaccatc acggtgagca
     7741 cgggcaacca ctcgaccacg atcagcaagg gtaatcacag cacgaccgtc agcatgggca
     7801 acatggacag caccgtgtcg atgggcaatc tctccaccga cgtcaaaatg ggcaacatca
     7861 gcacgaaggc cgatctgggc cagatctcca acgaggcgat gcagagcatc gagctgaagg
     7921 tcggcgccaa ctcgatcaag atcgaccaga tgggcgtaac gatcactggt atgatgataa
     7981 agatccaggg ccaggtgcaa acgcaggtgg aagggatgat gacgcagatc agcgccaatg
     8041 cgatgctgca gctcagcggc cccatcacga tgatcggctg agatgtcccc gacagtcagc
     8101 aaactggcgg ccatcgatct ggccagcctg accccgcacg ttgggctcag cccggaggca
     8161 tccgcggcga cggccggcag tggcaaggtc gaggacgcgc tcgcaaagct ggaagctagc
     8221 ggctttctca tggaggcgac caggctgatg gcgcacgcgc tgccgaagcg cgaagcggtc
     8281 tggtggagct gcatgtgctc cagccacacg gcgccacccg atctgccgga gcccgatcag
     8341 aactgccgcc gtgcggccga ggagtgggta cgcggccaga acgagaagct gcgctatgcg
     8401 gcgtgggaca cggcgcaggc cagcggctat ggcacgccgg aggtgtatac ggcggttgcc
     8461 gcgttctgga gcggtggctc gatgtcaccg gagggacagc ctgccgtacc gccgcagccg
     8521 catttcaccg gcaacgcggt ggccggcgtg atcgcgcttt ccgcggtgcg cggcgacccg
     8581 acgaagcggg atgcgcgatt gaagcgattt ctggaaagtg ggcgtaatat cgcggc
//
LOCUS       DATGEM010000400         9681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1344758, whole genome shotgun sequence.
ACCESSION   DATGEM010000400 DATGEM010000000
VERSION     DATGEM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344758"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VKQ27_12795"
     CDS             <1..403
                     /locus_tag="VKQ27_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HLY89851.1"
                     /translation="LKVGDTHSERVAENLSRTQIVMYCGASGDYNPLHADEVYVTQKA
                     GYPGVFAHGMLTMGLTGKMLTNYVGDGRLTKFGVRFTNQVWPGDDLDAKATIAALREE
                     NGQHFVDLTLSTTNQDGKEVASGSATARIDP"
     gene            403..2145
                     /locus_tag="VKQ27_12800"
     CDS             403..2145
                     /locus_tag="VKQ27_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY89852.1"
                     /translation="MTDPRSGGALRQRVTTALAMPLDSVTRAAEMRRVVLTSGERDVT
                     RLIAQLDAAAKTLGHDIELSLRLEGQLDAAGLELLREQGRKLKLGYIADPCADLAQAC
                     AAFAEGAPPLAISAHRHEPQALALAMRDGGVQVLLVRPDTARMWSVAADVTGIELAVI
                     GDTSLASELRAATQPVIVQSPTPDATYPPSKINKIARITLHRVKVPMRQLYVSALYMR
                     RTTERIVIELETADGQRGFGETNGTDEVLHIVQRMAQKLIGQDPLDRLRLRHLIAGPT
                     VGSRNGLAEWAALAGLDMALFDWAGRHHNMPIWQLLGGSGARRDFEAVAHIPALLLDE
                     PIDRGELPRRFADPATVHRIVEHALDLHRDPGFTAFKMKCTGTSPEWDVRILAALREA
                     LGPEVKLRWDPNASYPPAQATVLCQQLEPLDLEYYEDPIHGIPGMAQLRRRMKTPLAT
                     NMCVVTFDHLVHFLHQPCVDVVLADLVMWGGIQGMAELGAVAPLFGFDMTIHSAFECG
                     LGMAANLQVAAALEPVRRAIDFGLENMVNELIVPHIPVRDGRVAVPDGPGLGVQPNWS
                     EINQLKMDTTVVTG"
     gene            2228..2665
                     /locus_tag="VKQ27_12805"
     CDS             2228..2665
                     /locus_tag="VKQ27_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLY89853.1"
                     /translation="MTTVAAILKHKGNQVAFVDPTVTIARVSEVLVEHRIGAVLVMDR
                     ADQLLGIVSERDIVRCLAANGVRTLEMTAGQLMTRALKVAAPETTVGEAMTMMTVGRF
                     RHMPVIDRDTLVGVVSIGDVVKARIMEQEDEVDSLKAYVSGSV"
     gene            complement(2669..2977)
                     /locus_tag="VKQ27_12810"
     CDS             complement(2669..2977)
                     /locus_tag="VKQ27_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89854.1"
                     /translation="MRSVITVGAFASILLLGACGTEQPDRAQGGAATGAATGAAIGIV
                     GGPVGIVVGGLIGGAAGATTGAVTKPETVDLGPPPWSDQYGYQQPPQPMPAPPPPQQP
                     "
     gene            3118..3531
                     /locus_tag="VKQ27_12815"
     CDS             3118..3531
                     /locus_tag="VKQ27_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89855.1"
                     /translation="MPIIDRREIEFDREALRLVLECSPKAAQAFGLPSRRPDRVRCKP
                     AEGLVEVSYGKLTETRVFMLRAEALGAILLAYCNRIGMPMPRAADKAVRIEREHAVMV
                     FILRMPHAPHPDSPEGPVNRLPDAVRVFSWVEGSG"
     gene            3592..5619
                     /locus_tag="VKQ27_12820"
     CDS             3592..5619
                     /locus_tag="VKQ27_12820"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HLY89856.1"
                     /translation="MTNRIELRIAERIPFADSHPFGDVGAYERLTGRAHFAVDPRAPA
                     QADVVDLDKAPRDDAGLAHFAADFMILKPVELERGNRRVFYDYGNRGHKRALQFYNDA
                     QHSNAPITLAHAGNGFFMRRGYAVAWLAWEGDMLPGDGRMVLDVPVATDNGKPITGRI
                     RVEYIVDHPGTTCFPLSGRIAAHSYATVSLDTRDAVLTRRRYPYDTPLVIPSDQWAFA
                     SVESGSGGETQTPERAIVPSDRHIHLPAGFQPGWIYELVYTAKDPLVHGLGHVAVRDF
                     ISFLKYDRSDANPLRGTEKAYAWGRSQTGRCLRDFVYRGFNADAQGRRVFDGVLPHVA
                     GAGRKWLNHRFASPIVSGGQQYEDHFNIADSFPFSYAWSTDHLTGKQDAILKRPDTDP
                     LVFHTQTSTEYWVRRGSLVHTDTHGNDLKQPDTVRVYCWSSSQHFADPHPKKPSRGIG
                     QNFSNIVSTSMYFRANLDAMDRWATNGAPPPASRIPLRADNTLVPIEEWRRQFPSIPG
                     IATPASPNVLPLLDFGPDVDRGILREPPHPVPGGNSPDAPGKQYTVLVPSVDRDGNDV
                     PGVRAPMVGAPLGTYTGWNLRARGHGHGSQLRFEGSYIPFPDSPEERRVTGDPRTSVL
                     ERYPDKAAYVAAIAAAAHELVAQGLMLEEDVARCAEAAADWGRPRHDVSLD"
     gene            5645..7399
                     /locus_tag="VKQ27_12825"
     CDS             5645..7399
                     /locus_tag="VKQ27_12825"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLY89857.1"
                     /translation="MIDPTYLSSPARANRPNVMARHHVVVSGHYYASLAGLQILEAGG
                     NAVDAGVATGLAIDVLESEFVGFGGVAPVMIHQAERNERHVISGVGVWPKAASIEHFH
                     RNHGGRIPEGLLHCVVPAAPSIWITALARFGTMSFGEVAAAAIRFAREGFPTYPFLAE
                     QLAARVKDFAHWPTTAAIFLPNGKPPAAGDLFVQSDLARTLQYMADQERARANGDRLA
                     GLQAARDAFYRGDIATAILRHQRDNGGWIEVDDLADFHSPIEPACRIRFGEFDVYGCG
                     PWSQGPMVLEALNVLRGLDLRGMGHNSAAYIHAVTEALKLAAADREVYFGDPDFIDVP
                     LEALLSDVYADRRRAQINPAKAWPGMPPAGEVGGVSIPPWRPDPSAAPPLAIADTKPE
                     TSFLCVADRHGNVFAATPSDPTISGPVVPGTGITVSMWGSRGYTSPDHPARIGPGRRP
                     RMSANPAIAIKPGEMVMPFGSPGSEVLGQAMVQVFLNQAVFDMDPQSACEAPRFASYS
                     WPESSLPHSYRPGHLCVEEDISPATTDALSVLGHNVETWPSRKYLAGSVCTIQSDQRT
                     GLKWAGADPRRTAYAIGW"
     gene            7496..8014
                     /locus_tag="VKQ27_12830"
     CDS             7496..8014
                     /locus_tag="VKQ27_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HLY89858.1"
                     /translation="MIMAARHEHLGTPTDLGADATRDISAALNGLLADVFALYLKTKN
                     FHWHMSGPHFRDYHLLLDEHGDQIFAMTDPIAERVRKLGGNTIRSIGHISRQQRIPDN
                     DAAYVTPQDMLAELREDNRKLVSIMRAVHDTCDEHNDVASTSLLEVWIDETERRAWFL
                     FEMTQNRERDNN"
     gene            8115..9506
                     /locus_tag="VKQ27_12835"
     CDS             8115..9506
                     /locus_tag="VKQ27_12835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY89859.1"
                     /translation="MDTHTSRRWYALATVSAAQFLAVADAFIVNVAIPAIRADLHASG
                     AAIEAIIAVYQITYAALVITGGRLGDIFGRKPIFLLGVLGFTLASLWCGLAPSAAILI
                     LARCVQGATAALMVPQVLASIHTLFPDAARARAFSIFGLAIGLGAAVGFMAGGWLVTL
                     DLAGMGWRAIFCVNVPIGLAIIGAAAMLMPALPRVADARLDVPGAAVLLLGLLGLIVP
                     LMLGREFAWPWWCWLMIAAGIAALAGFLRLERRVANPLIDLSLLDDRVFLLGMAATAC
                     FFLGNLSFYFVLTLYMQNGLGFSAFNAALTVLPLSFAFVIGTRRSGGRLIEGCIVQAL
                     GLAATAALVATVTQPTMWMLMAALAMFGYGQGMVLAPLFNAVLTNVRHTHAGSGAGIL
                     TTTQQIANGTGVVIVGAIYLSVHTLHGDRTAMLTSLAVLACTIVGTIVALVTCGSAAR
                     SPAPETRSGSPAE"
     gene            complement(9442..>9681)
                     /locus_tag="VKQ27_12840"
     CDS             complement(9442..>9681)
                     /locus_tag="VKQ27_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLY89860.1"
                     /translation="ITTLGFDPVWFGVIIVMTVELGLIHPPVGMNVFVIKTVVKDVAF
                     CTIFIGVIPFVITDIIRLAILIAFPALAIWLPNHM"
BASE COUNT         1639 a         3304 c         3011 g         1727 t
ORIGIN      
        1 actgaaagtc ggcgacacgc acagcgagcg cgtcgccgag aacctctcgc gcacccagat
       61 cgtcatgtat tgcggcgctt cgggtgacta caacccgctc cacgccgacg aggtctatgt
      121 gacgcagaag gcaggctatc ccggcgtgtt cgcgcacggc atgctgacaa tgggcctgac
      181 cggcaagatg ctgaccaatt atgtaggtga cggccgtctg accaagttcg gtgtgcgttt
      241 caccaatcag gtgtggccgg gcgacgacct ggatgcgaag gccaccatcg ctgcgctgcg
      301 cgaggagaac ggccagcact tcgtcgacct tactctgtcc accaccaacc aggacggcaa
      361 ggaggtcgcc agcggttcgg cgactgcgcg gatcgatccg taatgactga cccgcgatcc
      421 ggtggcgcgc tccgccagcg cgtcaccacc gcgctggcga tgccgctgga tagcgtgacg
      481 cgggcggccg agatgcgccg cgtcgtgctg accagcggcg agcgcgacgt cacccgcctg
      541 atcgcacagc tcgacgccgc cgcgaaaacc ctgggccacg acatcgagct ttcgctccgc
      601 ctcgaaggcc agctcgatgc agcagggctg gaactgctgc gcgagcaagg gcgcaaactg
      661 aagctcggct acatcgcgga cccgtgcgcc gatctcgcgc aagcctgcgc cgccttcgcg
      721 gaaggggctc ctcctctggc aatctctgcg catcgccatg agccgcaggc gctggcgctg
      781 gccatgcgcg acggcggcgt gcaggtcctg ttggtacggc cggacacggc acgcatgtgg
      841 tctgttgctg ccgatgtcac cggcatcgag cttgcggtca tcggtgacac atcgctcgca
      901 tcggaactgc gtgccgccac gcaaccggtg atcgtgcagt ctccgacgcc cgatgcgacc
      961 tatccacctt ccaaaataaa caaaatcgcc cgcatcacgc tccaccgggt gaaggtgccg
     1021 atgcggcagc tctacgtcag cgcgctgtat atgcgccgta ccaccgagcg catcgtcatc
     1081 gagctggaaa ccgccgacgg ccagcgcggc ttcggcgaga ccaacggcac cgacgaagtg
     1141 ctccacatcg tccagcgcat ggcgcagaaa ctgattggtc aggacccgct cgatcgcctg
     1201 cgcctgcgcc acctgatcgc cggtcccaca gtcggctccc gcaatgggct ggcggaatgg
     1261 gccgcactcg ccggcctcga catggcgctg ttcgactggg cgggccgcca ccacaacatg
     1321 cccatctggc agctgctcgg cgggagtggc gctcgccgcg acttcgaagc tgtcgctcac
     1381 attcctgcgc tgctgctcga cgaaccgatc gatcgcggcg aactgcctcg ccgctttgcc
     1441 gatcccgcga ccgtgcatcg gatcgtcgag cacgcgttgg atctgcatcg tgacccgggc
     1501 ttcaccgcat tcaagatgaa atgcaccggc accagcccgg agtgggacgt gcgcatcctc
     1561 gccgcgctgc gcgaggcgct cgggcccgag gtgaaactgc gctgggaccc caatgcctcg
     1621 tatccgccag cgcaggcaac ggtgctgtgc cagcagctcg aacctctgga cctcgaatac
     1681 tacgaggatc cgatccacgg catccccggc atggcgcaac tgcgccggcg catgaagacg
     1741 ccgctcgcca ccaacatgtg tgtggtgaca ttcgatcacc tggtgcactt cctgcaccag
     1801 ccctgtgtcg atgtcgtgct ggccgacctg gtgatgtggg gcggcatcca gggaatggcg
     1861 gaactcggtg ccgtcgcgcc gctgttcggc ttcgacatga ccatccacag cgcgttcgaa
     1921 tgcggcctcg gtatggccgc caatcttcag gtcgcggcag cgctcgagcc ggtgcgccgc
     1981 gccatcgatt tcggcctgga gaacatggtg aatgagctga tcgtgccgca catcccggtg
     2041 cgtgatggcc gcgtcgcggt gcccgatgga ccaggtctcg gcgtgcagcc caactggtct
     2101 gaaatcaatc aactgaaaat ggatacaact gtcgtcacgg gctgaattgt gcagtgcaca
     2161 cctacaagtt gcacacgcac ttcccgtgag cgcatgctcg cttgcctcgc agcaggggaa
     2221 caaagcgatg acgacagttg ccgcaatcct caagcacaag ggcaatcagg tcgcctttgt
     2281 cgatcccacc gtgaccatcg cgcgtgtgtc ggaggtgctg gttgagcacc gtattggcgc
     2341 cgttctggtg atggatcgcg ccgatcaact tctcggcatt gtgagtgagc gcgacatcgt
     2401 tcgctgcctc gctgccaacg gtgtgcgcac gctggaaatg actgccggcc agctgatgac
     2461 tcgcgcgctc aaggtcgcag cgcctgaaac caccgtgggc gaagccatga ctatgatgac
     2521 tgtcggccga ttccgtcaca tgccggtaat cgaccgcgac acactggtgg gcgtcgtcag
     2581 catcggcgac gtcgtgaagg cgcgcattat ggagcaggaa gacgaggtgg acagcctgaa
     2641 agcgtacgtg tccggctcgg tctgacggtt acggctgctg gggcggaggt ggcgccggca
     2701 ttggctgcgg tggctgctga tacccatact ggtcgctcca cggcggcggg ccgagatcga
     2761 cagtctccgg cttggtcacc gcgcctgtcg tcgcaccggc ggcgccgcca atcagtccgc
     2821 ctaccacaat gccaacgggt ccgccgacaa tgccgatcgc cgcgccggtt gcagcaccgg
     2881 tcgccgcgcc gccttgcgcg cggtcaggct gctccgtgcc gcacgcgcca agcagcagaa
     2941 tgctggcaaa cgctccgact gtgatgacac tgcgcattgc aaccgttccc ctttgatcag
     3001 ttcgcatcgt aggcgggaag cgcacaattc atggcattat cgtgacgaaa tacgccaact
     3061 tcaccccgcg ttcattttca tcagacatga tcgcgattcg atgcaaggac actgtcgatg
     3121 ccgatcatcg atcggcggga gatcgaattc gatcgcgagg cgctccggct cgtcttggaa
     3181 tgctcgccga aggcagctca ggcattcggt ctgccgtcgc gccggcctga tcgcgtccgc
     3241 tgcaagcccg ccgaaggtct ggtcgaagtc tcctacggca aactgaccga aacgcgtgtg
     3301 ttcatgctgc gcgccgaggc cctgggcgcc atcctgcttg cctattgcaa tcgtatcggc
     3361 atgccgatgc cgcgcgccgc cgataaggcc gtgcgcatcg aacgcgagca tgccgtcatg
     3421 gtcttcatcc tgcgcatgcc gcatgcgccg cacccagact caccggaagg gccggtcaat
     3481 cgtctgcccg atgcggtacg ggtcttctcc tgggtggaag ggagcggctg acacccttgc
     3541 cgcacatcat gacgggccgc acagtgccgc gggcagtgtg ggggatacgg catgaccaat
     3601 cggatcgaac tgcggattgc ggaacggatc ccgttcgccg acagccaccc gttcggcgat
     3661 gtcggcgctt acgagcgact gaccggccgt gcgcatttcg ccgtcgatcc gcgtgcaccg
     3721 gcgcaggcgg atgtggtgga tctcgacaag gctccgcgtg acgatgccgg actggcccat
     3781 ttcgctgccg acttcatgat cctgaagccg gtcgaactcg aacgcggcaa tcgccgcgtc
     3841 ttctacgact acgggaaccg aggccacaag cgagcgctcc agttctacaa cgatgcccag
     3901 cacagcaacg cgccgatcac gcttgcccac gccggcaacg gcttcttcat gcgccggggc
     3961 tacgcggtgg catggctcgc ctgggaaggc gacatgctcc cgggcgatgg ccggatggtg
     4021 ctcgacgtgc cggtggccac cgacaacggc aagccgatca ccggacgtat ccgcgtcgaa
     4081 tatatcgtgg atcacccagg tactacgtgc tttccgctca gcggccgtat cgccgcgcac
     4141 agttatgcca ccgtgtcgct cgatacgcgc gacgcagtgc tgacccgccg ccgctatccg
     4201 tatgacacgc cgctggtcat cccctccgac cagtgggcct tcgccagcgt cgaatccggc
     4261 agcggcggcg aaacgcagac gccggagcgg gccatcgtcc cctccgatcg gcacatccat
     4321 ctgcctgccg gcttccagcc cggctggatc tatgaactcg tctatacggc gaaggatccg
     4381 ctggtgcatg gcctgggtca cgtcgcggtg cgcgacttca tcagcttcct gaaatacgac
     4441 cgttccgatg ccaacccgct gcgcggcacg gagaaagcct atgcctgggg ccgctcgcag
     4501 accggtcgct gcctgcgcga cttcgtctat cgcgggttca atgccgacgc gcaggggcgc
     4561 cgcgtattcg acggggtgct gccgcacgtc gccggcgccg gccgcaaatg gctgaaccat
     4621 cgcttcgcca gccccatcgt ttccggcggc cagcagtacg aggaccattt caacattgcc
     4681 gacagcttcc cgttttctta cgcttggtcc accgatcatc tcaccggcaa gcaggacgcg
     4741 atcctgaagc gcccagacac cgatccgctg gttttccaca cccagacttc caccgaatac
     4801 tgggtgcgcc gaggctccct ggtgcacacc gacacgcacg gcaacgacct gaagcagccg
     4861 gacacggtgc gtgtttattg ctggtcaagt tcgcagcact tcgccgatcc gcacccgaaa
     4921 aagccgtcgc gcggtatcgg ccagaatttc tccaatatcg tatctacgtc aatgtacttc
     4981 cgcgccaatc tcgacgcgat ggaccggtgg gccaccaacg gcgcgccgcc gcccgccagc
     5041 cgcatcccgc tgcgcgcgga caacacgctg gtgcccattg aggaatggcg ccgccagttc
     5101 ccctccatcc ctggcatcgc cacacctgcc tcgccaaacg tgctgccgct gctggatttc
     5161 ggtcccgacg tggaccgcgg catcctgcgc gagccgccgc atcctgttcc tggcggcaac
     5221 tcgcctgacg cgccgggcaa gcagtacacg gtgctggtgc cctcggtgga tcgcgacggc
     5281 aacgacgtgc ctggcgtgcg cgcgccgatg gtaggcgcgc cgcttggcac ttacaccggc
     5341 tggaacctac gtgcgcgcgg tcacggccac ggctcccagt tgcgcttcga gggcagctac
     5401 atcccgttcc ccgactctcc cgaggagcgt cgcgtcaccg gcgatccgcg cacatcggta
     5461 ctggagcggt atcccgacaa ggcggcctac gtcgccgcaa tcgccgctgc tgcgcacgaa
     5521 ctggtcgcgc aggggctgat gcttgaggag gatgtggcgc gctgcgctga agctgccgcc
     5581 gactgggggc gtccgcggca cgatgtttcg ctggattgac acgcactcaa gaaggctttc
     5641 gaccatgatc gatccaacct acctctccag tcccgcccga gccaatcgcc caaacgtcat
     5701 ggcgcgccac catgtcgtgg tctccggcca ctactacgcc tcgctcgcgg ggttgcagat
     5761 cctggaagcc ggcggcaatg ccgttgatgc gggcgtcgcc accgggcttg ccatcgacgt
     5821 gctggaaagc gaattcgttg gcttcggcgg cgttgccccg gtgatgatcc atcaagccga
     5881 acgcaacgaa cgccacgtga tcagcggcgt cggcgtctgg cccaaggccg ccagcatcga
     5941 gcacttccat cgcaaccacg gcggccgcat ccccgagggc ctgctgcact gcgtcgttcc
     6001 cgccgccccc agcatctgga tcaccgcgct ggcccgtttc ggtaccatgt cgttcggcga
     6061 ggtcgccgcc gccgcgatcc gcttcgcgcg tgagggcttc cccacctatc cattcctcgc
     6121 cgagcagctt gcggcgcgcg tgaaggattt cgcccactgg cccaccaccg ccgcgatctt
     6181 cctgcccaac ggcaagccgc cggcggcggg cgatctgttc gtgcagagcg atcttgcgcg
     6241 cacgctgcaa tacatggcgg accaggagcg cgcccgtgcg aacggcgacc gcctcgccgg
     6301 tctgcaagcc gcgcgcgacg cgttctaccg gggcgacatc gctaccgcga ttcttcgcca
     6361 ccagcgcgac aacgggggct ggatcgaagt cgacgatctc gccgacttcc acagcccgat
     6421 cgaacccgcg tgccgcatcc gcttcggtga gttcgatgta tatggctgcg gcccttggtc
     6481 tcaaggacca atggtgctgg aggcactgaa tgtcctgcgt ggcctcgatc tgcgcggtat
     6541 gggccacaac agtgctgcgt atattcacgc ggtgaccgag gcgttgaagc tcgctgccgc
     6601 cgaccgcgag gtttatttcg gcgatcctga tttcatcgac gtgccgctgg aagcgctgct
     6661 gtccgatgta tacgccgatc gtcggcgcgc ccagatcaat ccagccaagg cctggccggg
     6721 aatgccgccg gcaggcgagg tgggcggcgt cagcattccg ccctggcgcc ccgatccctc
     6781 cgcagccccg ccgctcgcga tcgccgacac gaaaccagag acgtcgttcc tgtgcgttgc
     6841 cgatcgccac ggcaatgtct tcgccgctac gccgagcgat ccgacgatca gcggccctgt
     6901 cgtgcctggc accgggatca ctgtctcgat gtggggttcg cgcggttaca cgtcgcccga
     6961 ccatccggcc cgcataggcc ctggccggcg tccgcgcatg tccgccaatc cggccatcgc
     7021 gatcaagccg ggcgagatgg tgatgccgtt cggctccccg ggcagcgagg tactgggcca
     7081 ggcaatggtg caggtgttcc tcaatcaggc ggtattcgac atggacccgc aatccgcctg
     7141 cgaagcgcct cgtttcgcct catactcctg gcctgagtca tcgctgccgc attcctaccg
     7201 ccccggccac ctctgcgtgg aggaagatat cagtccggct acgaccgacg cgttgtcggt
     7261 gctcggtcac aacgtggaga cgtggccgtc gcgtaaatac cttgccgggt cggtgtgcac
     7321 gatccaatcc gatcagcgaa cgggcctgaa atgggcgggt gccgatccgc ggcgcactgc
     7381 ctacgcaatc gggtggtagc cgctggcaca gccgcgcgta tcggcaaaga cagcgcaaag
     7441 agaacagacg ctgccatcca tggttgggat actccagaca tcccgatcaa ggagcatgat
     7501 tatggcagca aggcatgagc acctgggcac gcccaccgat ctgggcgcgg atgcgacgcg
     7561 cgacatttcg gctgcgctca acgggttgct cgcggacgtc ttcgcgctct atctgaagac
     7621 caagaacttt cattggcaca tgagtggtcc gcatttccgc gactaccacc tgctgctgga
     7681 cgagcacggc gatcagatct tcgccatgac cgaccccatc gccgagcgtg tgcgcaagct
     7741 tggcggcaac acgatacgct cgatcgggca tatttcccgg cagcagcgga ttcccgacaa
     7801 cgacgccgcc tacgtgacgc cgcaggacat gctcgccgag ctgcgcgagg acaaccgcaa
     7861 gctggtgtcc atcatgcgcg ccgtgcacga cacctgcgac gagcacaacg acgtcgccag
     7921 caccagcctg ctcgaggtct ggatcgacga gaccgaacgg cgcgcctggt tcctgttcga
     7981 gatgacgcag aacagagagc gcgacaacaa ctgagttgcg ccgcggcggc ggccgcacat
     8041 gattacccgt cacgtaatcg attggcgcgg cgggcgccgc catcctgggg accatcatca
     8101 acggggttcc caatatggac acgcacacct cgcgtcgctg gtatgccctg gcgacagtct
     8161 cggcggcgca gttcctggct gtcgccgacg cctttatcgt caacgtcgcc atcccggcga
     8221 tccgcgccga cctgcatgcc agtggtgctg cgatcgaggc gatcatcgcg gtctatcaga
     8281 tcacttacgc cgcgctggtg atcaccggcg gacgcctcgg tgacatcttc ggccgtaagc
     8341 ctatcttcct gcttggcgtc ctcggtttca cactggcctc gctgtggtgc ggcctggccc
     8401 cctccgccgc gatcctgatc ctggcgcgct gcgtccaggg tgcgacagcg gcactgatgg
     8461 tcccgcaggt tctcgcgtcc atccacacgc tgttccccga cgccgcgcga gcacgcgcct
     8521 tctccatctt cggcctcgct atcggcctcg gcgccgcggt gggcttcatg gcgggcggct
     8581 ggctggtcac gctggatctc gccggcatgg gctggcgcgc gatcttctgc gtcaacgtgc
     8641 ccatcgggct ggcgattatc ggcgctgccg cgatgctgat gcccgcgctt ccccgcgttg
     8701 ccgacgcgcg gctcgacgtg ccgggcgcgg ctgtcctgtt gctggggctg ctcgggttga
     8761 tcgtgccgct gatgcttggc cgcgagttcg cctggccctg gtggtgctgg ctgatgatcg
     8821 ccgctggcat cgccgcgctg gcaggcttcc tccgcctgga gcggcgtgtc gccaacccgc
     8881 tgatcgacct gtcgctgctg gatgaccggg tattcctgtt aggcatggcc gcgaccgcgt
     8941 gcttcttcct gggcaacctg tcattctact tcgtgctgac gctgtatatg cagaacggcc
     9001 tcggcttctc cgccttcaac gccgcgctga ccgtgctgcc gctgtccttc gcattcgtca
     9061 tcggcacgcg tcgcagcggt ggcaggctga tcgagggctg catcgtgcag gcgctcggac
     9121 ttgccgcgac ggcggcgctg gtcgccaccg tcacgcagcc caccatgtgg atgctgatgg
     9181 cagcgctggc gatgttcggc tacggccagg gcatggtgct ggcgccgctg ttcaacgccg
     9241 tgctgaccaa cgtgcgccac acgcatgccg gatcgggcgc cggcatcctt accaccacgc
     9301 agcagatcgc caacggcacc ggcgtggtga tcgttggcgc tatctatctg tccgtgcata
     9361 cgctgcatgg tgaccggacc gccatgctga cctcgcttgc ggtgcttgcc tgcaccatcg
     9421 tcgggacgat cgtggcgctg gtcacatgtg gttcggcagc cagatcgcca gcgccggaaa
     9481 cgcgatcagg atcgccagcc gaatgatgtc ggtgatcacg aacgggatca cgccgatgaa
     9541 gatcgtgcag aaggcaacgt ccttcaccac ggtcttgatg acgaacacgt tcatccccac
     9601 gggaggatgg atcagcccaa gctcgaccgt catcacgatg atcacgccga accacaccgg
     9661 gtcgaacccc agtgtggtga t
//
LOCUS       DATGEM010000401         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1695523, whole genome shotgun sequence.
ACCESSION   DATGEM010000401 DATGEM010000000
VERSION     DATGEM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695523"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VKQ27_12845"
     CDS             complement(<1..249)
                     /locus_tag="VKQ27_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89861.1"
                     /translation="MLQAVGLAAFRGEKLVFRDLDFSVPDGGALLLTGPNGSGKSTLL
                     RVLAGLLRPAAGEVHWDGSVAYVGHQDAVKPALTSAENL"
     gene            348..3044
                     /gene="acnA"
                     /locus_tag="VKQ27_12850"
     CDS             348..3044
                     /gene="acnA"
                     /locus_tag="VKQ27_12850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HLY89862.1"
                     /translation="MKAIGQDTLKTRRTLTVQGKSYEYFSLPEAAKTLGDISRLPVSL
                     KVLLENVLRFEDGSSYKVDDAKAIAAWLPSAHSDREVPFRPARILMQDFTGVPAVVDL
                     AAMRDGITRLGGSPQKVNPLVPVDLVIDHSVQVDVFARPDALQKNVDIEFERNAERYR
                     FLRWGQDAFANFRVVPPGTGICHQVNLEYLGQCVWTGEADGKAIAYPDTLYGTDSHTT
                     MVNGLGILGWGVGGIEAEAAMLGQPIAMLIPDVIGFKLTGRLPEGATATDLVLTVTQM
                     LRRKGVVGKFVEFFGPGLDQLGLPDRATIGNMAPEYGATCGFFPVDKVALDYLHLSGR
                     DEHRIALAEAYLKAQGMFREPGAPDPVFTDTLELDLSTVQPSMAGPKRPQDRVLLKEV
                     AASFKADLTKGLGVPANEAGTSVKVEGKNYELTHGDVVIAAITSCTNTSNPSVLVAAG
                     LVARKAHAKGLKPKPWVKTSLAPGSQVVTEYLDKSGLTKDLDAIGFETVGYGCTTCIG
                     NSGPLDDAIVDAIEDNRLVGAAVISGNRNFEGRVHANVRANYLASPPLVVAYSLLGKV
                     TADITTEPLGTGNDGQPVYLKDVWPTNKEIADVVGSSLSRHQFLKRYGEVFKGPKQWQ
                     TIEVDTDSDTYRWSDGSTYVKNPPYFEGITMDPPPVGDVKGARILAELGDSITTDHIS
                     PAGSIRKVSPAGDYLLERQVTQANFNSYGARRGNHEVMMRGTFANIRIRNEMLSNVEG
                     GMTRHLPDGEQMPIYDAAMKYKAEGVPLVIFGGKEYGTGSSRDWAAKGTFLLGVKAVI
                     VESFERIHRSNLVGMGVLPLTFKEGSDRKSLGITGEEIIDVIGLEQAIMPGMDLSLVI
                     HRPDGKTDTVPVTCRIDTVDEVAYYKHGGILQYVLRGMAKAA"
     gene            3200..3745
                     /locus_tag="VKQ27_12855"
     CDS             3200..3745
                     /locus_tag="VKQ27_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HLY89863.1"
                     /translation="MSDKLTRSIEALEERIQRNLGRAMFAARWIMAPIYVGLLVALLM
                     IVVKFVQKLVTAIPGFLALSSNEAIFLVLTLVDLSLVANLVVIVIFAGWENFVGRLLA
                     SQHERPHWLGGLDFGAIKLRLIASIAAIAAILILETFVHIGEVPYQYAAWQLAILLGI
                     GVLGVLLALADRLTEPHDREK"
     gene            3747..4805
                     /locus_tag="VKQ27_12860"
     CDS             3747..4805
                     /locus_tag="VKQ27_12860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89864.1"
                     /translation="MQVGIQFFPDVGPEVKSARDYWQEALVLVGLCDRYGYSHVRTVE
                     HYFHPYGGYSPNPVVFLAAAAQVTKRARLVTGAVLPAFNNPLKLAGELAMLDALCDGR
                     LDIGFARAFLPHEFARFGVKLDESRARFDEGVEQVRLLLEQENVTHEGRFHSFRDLTS
                     LPRPTQRPRPPFYVAALATRESFERAGAAGHGIMAIPMGGGAMAELINTYRDAWKRAG
                     HPGKGVVMLAFHMFCHQDQATAERIAREPLNRYLRSLVEAASEWMSGESSADYPGYDK
                     IIAMLSRDDFDSQVAKGAAWVGTPNRILDSVASYRQQIGDFEIASLQVNFNDMSLADA
                     QASMRLFGEEVLPRLPRL"
BASE COUNT          890 a         1563 c         1590 g          875 t
ORIGIN      
        1 caggttctcc gctgaggtca gcgccggctt cactgcgtcc tgatgcccga cataggcgac
       61 cgatccgtcc cagtgcactt cgccagccgc cggccgcaac agtcccgcca gcacgcgcag
      121 cagcgtcgat ttgccagacc cgttcggtcc ggtgagcagc aatgcgccgc cgtctggcac
      181 ggagaaatcc agatcacgga acaccagctt ttcaccgcgg aacgctgcca gacccaccgc
      241 ctgcaacaaa agccgctctc cgccttgtgg acgcttgcag gctggtgtgt tctaagcccg
      301 ccgccgcaga ccattgcatc tgcggcatac caacggaagg cgttgccatg aaagcaatcg
      361 ggcaggacac cctgaagact cgccgcacgc tcactgtgca gggcaagagc tacgaatact
      421 tctcccttcc cgaggcggca aagacacttg gcgacatctc ccgattgcct gtcagcctga
      481 aggtgctgct ggagaatgtg ctgcggttcg aggacggcag cagctacaag gtggacgatg
      541 cgaaggcgat cgcggcgtgg ttgccgtccg cgcattccga cagggaagtg ccattccggc
      601 ctgcgcgcat cctgatgcag gatttcaccg gtgtgcctgc ggtggtggat ctggctgcga
      661 tgcgcgacgg cattacccgc ttgggtggct cgccgcagaa ggtgaacccg ctggtgccgg
      721 tggatctggt gatcgaccac tcggtgcagg ttgatgtgtt cgcccggccc gatgcgttgc
      781 agaagaacgt cgatatcgaa ttcgagcgca acgctgagcg ctaccgcttc ctgcgctggg
      841 gccaggacgc gttcgccaat ttccgcgtgg tgccgccggg caccggcatc tgccaccagg
      901 tgaacctgga atatctgggc cagtgtgtct ggaccggcga ggcggacggc aaggccatcg
      961 cctaccccga cacgctgtac ggcaccgaca gccacaccac gatggtgaac ggccttggca
     1021 tcctcggctg gggcgtgggc ggcatcgaag ccgaggcagc gatgcttggc cagccgatcg
     1081 cgatgctgat ccccgacgtc atcggattca aactgacggg acggctgccg gaaggcgcca
     1141 ccgccaccga ccttgtgctg acggtgacgc agatgctgcg gcgcaagggc gtcgtgggca
     1201 aattcgtcga gtttttcggg cccgggctcg atcaactcgg gctgccggat cgcgccacca
     1261 tcggcaacat ggcgcccgaa tacggcgcga catgtggctt cttcccggtc gacaaggtgg
     1321 cgctcgacta tctgcacctg tcgggacgcg atgagcatcg catcgcgctg gcagaagcgt
     1381 acctgaaggc gcagggcatg ttccgcgagc ccggcgcacc cgatcccgtg ttcaccgaca
     1441 cgctggaact cgatctctcg acggtgcagc cctcgatggc cggcccgaag cggccgcagg
     1501 atcgcgtgct gctgaaggag gttgccgcca gcttcaaggc ggatctgacc aagggcctcg
     1561 gcgtgccggc gaacgaggcc ggcacatcgg tgaaggtgga gggcaaaaac tacgagctga
     1621 cccacggcga cgtggtcatc gcggcgatca cctcctgcac caacacgtcg aacccgtccg
     1681 tgctggtggc ggccggtctg gtggcccgca aggcgcatgc gaaggggttg aagccgaagc
     1741 cgtgggtgaa gacctcgctc gcgcctggat cgcaggtggt gacggaatat ctcgacaagt
     1801 cgggcctgac caaggacctc gacgcgatcg gtttcgaaac cgtcggctac ggctgcacga
     1861 cctgcatcgg caactcgggc ccactggatg atgccatcgt ggatgcgatc gaggacaaca
     1921 ggcttgtcgg cgccgcggtg atctccggca atcgcaattt cgagggccgc gtgcatgcca
     1981 atgtgcgggc gaactatctg gcatcgccgc cgctggtggt ggcctattcg ctgcttggca
     2041 aggtgacggc cgacatcacc accgagccgc tgggaaccgg caatgacggc cagcccgtgt
     2101 atttgaagga cgtctggcca accaacaagg aaatcgccga cgtcgtaggt tcgtcgctgt
     2161 cgcgccatca gttcctgaag cgctacggcg aggtgttcaa gggaccgaag cagtggcaga
     2221 cgatcgaggt agatacggat agcgatacgt atcgctggtc cgacggctcc acctacgtga
     2281 agaacccgcc ctatttcgag ggcatcacga tggatccgcc gccggtgggc gatgtgaagg
     2341 gtgcgcgcat cctggcggag cttggtgact cgatcaccac cgatcacatc agccctgccg
     2401 gttccatccg caaggtgtcg cccgccggcg actacctgct ggagcgccag gtcacgcagg
     2461 cgaacttcaa cagttacggc gcgcggcgcg gcaatcatga agtgatgatg cgcggcacct
     2521 tcgccaacat ccgtatccgc aacgaaatgc tgagcaacgt cgaaggtggc atgaccaggc
     2581 acctgcccga tggcgagcag atgccgatat acgatgcggc gatgaagtac aaggccgagg
     2641 gcgtgccgct ggtgatcttc ggcggcaagg aatacggcac cggttcgtcg cgcgactggg
     2701 cggcgaaggg gacgttcctg ctcggcgtca aggcggtgat cgtggagagc ttcgagcgca
     2761 tccaccgctc caacctcgtc ggcatgggtg tgctgccgct gaccttcaag gaaggctccg
     2821 accgcaagtc gctcggcatc accggcgaag agatcatcga tgtcatcggt cttgagcagg
     2881 cgatcatgcc ggggatggac ctgtcgctgg tgatccaccg tccggatggc aagaccgaca
     2941 ctgtgccggt gacctgccgc atcgataccg ttgatgaagt cgcctactac aagcacggcg
     3001 gcatcctgca gtacgtgctg cgcgggatgg cgaaggcggc ctgacgcgcc cgcgcccgcc
     3061 gcaccggaag tggcaacgga acgggcgccg gtctggggcg ctcgatccgg cgcggttgcc
     3121 cctctgccta cgggaagact ggttgcgcta tacggtgccg catggacaat gatggcgcga
     3181 cacaagatgc ccggcatcca tgagcgacaa gctgacacgg tccattgagg cgctcgagga
     3241 gcgcatccag cgcaaccttg gccgcgccat gttcgccgcg cgctggatca tggcgccgat
     3301 ctacgtgggc ctgctcgtcg cgctgctgat gatcgtggtg aagttcgtac agaagctggt
     3361 gactgcgatc ccagggttcc tcgcattgtc cagcaacgag gcgatcttcc ttgtcctgac
     3421 gctggtcgat ctgtcgctgg tggcgaacct tgttgttatc gtcatcttcg ccggttggga
     3481 gaatttcgtc ggccgcctgc ttgcgtcgca gcacgaacgt ccgcattggc tgggtggcct
     3541 cgacttcggc gcaatcaagc tgcggctgat cgcttcgatt gccgcgatcg ccgccattct
     3601 gatcctggaa accttcgtgc atatcggcga ggtgccgtac caatacgccg cctggcaact
     3661 tgcgatcctg ctcggcatcg gtgtgcttgg cgtcctgctg gcgctggccg accggctgac
     3721 cgaaccgcac gacagggaga agtaacatgc aagtcggcat ccagttcttc cccgatgtcg
     3781 gccccgaggt gaagtcagcg cgagactact ggcaggaagc gctggtgctg gtcggtctct
     3841 gcgatcgcta tggctacagc catgtacgga cagtcgagca ctactttcat ccatacggcg
     3901 gctacagtcc caaccctgtc gtgttcctcg ctgccgccgc tcaggtcacc aagcgcgcac
     3961 gtctcgtcac cggcgcggtg ctgccagcgt tcaacaatcc gctgaagctc gccggcgagc
     4021 tggcgatgct ggacgcgctg tgcgacggcc gcctcgacat cggcttcgcg cgcgccttcc
     4081 tgccgcatga gttcgcgcgg ttcggcgtaa agctcgatga aagccgcgcg cggttcgacg
     4141 aaggcgtgga acaggtgcgt ctgctgctgg agcaggagaa cgtcacgcac gaggggcgct
     4201 tccatagctt ccgcgacctg acctcattgc cccgcccgac acagcgtcca cgcccgccct
     4261 tctatgtcgc ggcgctggcc acgagggagt cattcgagcg cgccggcgct gccggccacg
     4321 gcatcatggc catcccgatg ggtggcggag cgatggccga gctgatcaat acctaccgcg
     4381 atgcctggaa acgcgccggc catcctggca agggcgttgt catgctcgcg ttccacatgt
     4441 tctgccatca ggaccaggcc accgcggagc gcatcgcgcg cgagccgctg aaccgctatc
     4501 tgcgctcgct ggtcgaggca gcatccgagt ggatgtcggg cgaaagttcc gccgattatc
     4561 ctggctacga caagatcatc gccatgctgt cgcgcgacga tttcgattcg caggttgcca
     4621 agggtgctgc ctgggtcggt acgccgaacc gcatcctcga cagcgtcgcc agctatcgcc
     4681 agcagatcgg cgatttcgaa atcgcctcgc tccaggtgaa cttcaacgac atgtcgctgg
     4741 ccgacgcgca ggcatcgatg cggctgttcg gcgaggaagt gctgccgcgg ctgccgcgcc
     4801 tgtagctaca ggcagccgca cacgacatca gcccacgccg tcgtgcggtg tgtgcacgac
     4861 atgtggaaca tctgaccctt catcgcctcg acggccacat gatcgagttc gcggccat
//
LOCUS       DATGEM010000402         6427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_252257, whole genome shotgun sequence.
ACCESSION   DATGEM010000402 DATGEM010000000
VERSION     DATGEM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252257"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1510
                     /locus_tag="VKQ27_12865"
     CDS             59..1510
                     /locus_tag="VKQ27_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HLY89865.1"
                     /translation="MKDVKLLIGGKRLDAANKATTDRIDPFTGEIASRAAAATLEDAD
                     AAVAAAQAAFPAWKALSAGERRKRLLHAADLMDKAAPRFIECGTNETGATPMWIGFNV
                     MLSASMLREAASMTTQITGDIMPSEVPGNMAMGIRAPVGVVLGIAPWNAPVILATRAL
                     AMPLACGNTVVLKAAESCPALQALIGDVLHEAGLGDGVVNVVTNAPKDAASIVERLIA
                     NPAIRRVNFTGSTHVGRIVAQLSAKHLKPALLELGGKAPFLVLHDADLDEAAAAANFG
                     AFMNQGQICMSTERIIVDAKVHGAFVEKLAAKASRLVAGDPRRKDVHLGTLVSADAAT
                     RIKALVDDAVSKGARVVCGGQVEGAVMQATVVDGVTPQMKLYAEESFGPVVSVIRAKD
                     DEDAIRIANDSLFGLSSAVFSQDIPRALDIAGRIEAGICHINGPTVHDEANMPFGGVK
                     DSGYGRFGSKASIAEFTELRWVTLQKTHRHYPI"
     gene            1624..4551
                     /locus_tag="VKQ27_12870"
     CDS             1624..4551
                     /locus_tag="VKQ27_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY89866.1"
                     /translation="MKEARSENGELNAVFPTGGGEVGELIRGFDWAATSLGPISAWPQ
                     SLKTATNLLLLSPVPIVLLWGEDGIMIYNDAYSVFAGARHPKLLGSKVREGWAEISDF
                     NDNVMKVGLAGNTLAYKDQELTLIRRGRPEPVWMDLDYSPVLDESGRPGGVIAIVVET
                     TERVLADRRLRESEQRFRALATATSDAVYRMSPDWKEMRQLDGRGFLSDLESPSVAWM
                     DAYLFAEDQPAVLAVIEQAVRTRGIFQLEHRVRQADGSAGWTFSRAVPVFDEQGEIVE
                     WFGTATDVTGRRRAEARREALVRLTEEIQKLNNPVDIAFAASRILGETLGVSRVGYGT
                     VDPADDSFVVAREWNAPGVESLAGTLNLRDFGSFVDDLKLGKLIVISDAMLDPRTKDA
                     ADALIARYARSFVDVAVVEQDQAVAVFYVSHAEARDWSAEELGLIREVAARTRTAIER
                     QRAERKFQDLAEKLESQVEERTRERDRVWQNTQELSAVIDRDGIFVAVNPATTKLLGW
                     TEADLIGRSLFDFVLPDDLEATGGAFERARREALPAFQNRYRTKGGGFRWISWVAAPD
                     QELIFASGRDITAEKEAADALAVSEDALRQAQKMEAVGQLTGGLAHDFNNILAGISGS
                     LELMQRKLSQGRVGDLDRYLALAHGATRRAAALTHRLLAFSRRQTLDPKPTNINRLVA
                     GMEDLIRRSVGPEIDIGTDAADGLWNTLVDAGQLENALLNLCLNARDAMPDGGMLIIE
                     TDNHQFDGQAARERDLPPGQYVSLCVRDTGAGMTPDVVARAFDPFFTTKPIGQGTGLG
                     LSMVYGFARQSGGLARIETELGKGSKVCIYLPRHLAEVEEAVSDDAEAPRAVAGHTIL
                     VVDDEPLVRLFVTEVLGDLGYTTLEAEDAQAGLDILRSTAQIDFLVTDVGLPNGMNGR
                     QLADAARELRPGLKVLFITGYAENALLSEGHLGRGMQAMTKPFTMEELAVRLRDSLGE
                     GWA"
     gene            4583..5212
                     /locus_tag="VKQ27_12875"
     CDS             4583..5212
                     /locus_tag="VKQ27_12875"
                     /inference="COORDINATES: protein motif:HMM:NF033832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HLY89867.1"
                     /translation="MKEFLPSDSTLLKMKRSRPTTNTKDRDIREPLISWIASRHPADG
                     STGIIQEFEMPRPSARIDLAVVNGEMSGFEIKSDADTLIRLRSQVPAFSRFFDKVSLV
                     TTQRHLPCARKMIPEWWGIMVFKQEGAFRVVRSAKQNRNTDVSSLLFALTKAEIVEIA
                     QRAGLKMRISQKKADMTGSVVIEISESDLFVYARDIIRKRTDSVILQGS"
     gene            complement(5193..6332)
                     /locus_tag="VKQ27_12880"
     CDS             complement(5193..6332)
                     /locus_tag="VKQ27_12880"
                     /inference="COORDINATES: protein motif:HMM:NF025708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89868.1"
                     /translation="MAQVEFSYYSYFPCLQSSEAEHLAYRELADEDKEAILPVFEINQ
                     AKNEASLQESIDSISGAIGDRPFILDLSKDRAPPAYVQKNNPDHVRIAQTQALQDAYN
                     AAMTALLAPENGFVAWRNLIANFPNAIPVLQFTDPATQANSVLRQAVRLSDGGSECIA
                     VRISQDTNPAIFGLIGQIIAILESASRLLIILDCGQGRQMVTERVEFARQAIARILEE
                     IEPSQAPYVSAVCMSDFFTTPPNGDGVRVYENGAWGLWSQASEGFALLFGDYCGHRRL
                     KKNNTFMPGDWKARVTYPLDDAWLIYKHANSQDAQGWITGAQEIRDHEDFVGFPECWG
                     GTVLERASNDDIAGLQSGRFWDAAKINMHIHRQIRFSQEVMNPEE"
BASE COUNT         1272 a         1910 c         1995 g         1250 t
ORIGIN      
        1 ggtgtcgggc gatggaatga ttgtcggggc ctgacgcgcc tcaaagggag agaagcaaat
       61 gaaggacgtg aagctgctga tcgggggcaa gcgcctggac gccgccaaca aggcgaccac
      121 cgaccggatc gatcccttca ccggcgagat cgcctcgcgc gccgcggccg cgacgctgga
      181 agatgccgac gccgccgtcg ccgccgccca ggccgcattc ccggcctgga aggcgttgag
      241 cgccggcgag cggcgcaagc gtctgctgca cgccgccgat ctgatggaca aggcggcgcc
      301 ccgcttcatc gaatgcggca ccaacgaaac cggcgccacc ccgatgtgga tcggcttcaa
      361 tgtgatgctg tcggccagca tgctgcgcga ggcggcgtcg atgaccaccc agatcaccgg
      421 cgacatcatg ccttccgagg tgccggggaa catggcaatg ggcatccgcg cccccgtcgg
      481 cgtggtgctg ggcatcgcgc cctggaacgc gcccgtcatc ctcgccaccc gcgccttggc
      541 catgccgctg gcctgcggca ataccgtggt gttgaaggca gccgagagct gccccgcgct
      601 gcaggcgttg atcggcgacg tgctgcatga agccggcctg ggcgacggcg tcgtcaatgt
      661 ggtgaccaac gcgcccaagg acgcggcttc catcgtcgag cgcctgatcg ccaatccggc
      721 catccgccgg gtcaatttca ccggctcgac ccatgtcggc cgcatcgtcg cccaattgtc
      781 ggccaagcat ctcaagccgg ctctgctgga actgggcggc aaggcgccgt tcctggtgct
      841 gcacgatgcg gatctggatg aagcggcggc ggcggccaat ttcggcgcct tcatgaatca
      901 gggccagatc tgcatgtcga ccgagcgcat catcgtcgat gccaaggtgc atggcgcctt
      961 cgtcgagaag ctggccgcca aggcgagcag gctggtggcc ggcgatcccc gccgcaagga
     1021 cgtccatctc ggcacgctgg tcagcgcgga cgcggcgacc cgcatcaagg cgctggtcga
     1081 cgacgccgtg tcgaagggcg cgcgcgtcgt ttgcggcggc caagtcgaag gtgccgtgat
     1141 gcaggcgacc gtggtcgacg gcgtgacgcc ccagatgaag ctctatgccg aggaatcctt
     1201 cggtccggtg gtgtcggtga tccgcgccaa ggacgacgag gacgccatcc gcatcgccaa
     1261 tgattcgctg ttcggcttgt cgtcggccgt gttcagccag gacatcccgc gcgcgctcga
     1321 catcgccggg cggatcgaag ccggcatctg ccacatcaac ggcccgaccg tgcatgacga
     1381 agccaacatg ccgttcggcg gggtgaagga tagcggctat ggccggttcg ggtcgaaggc
     1441 gtcgatcgcg gaattcactg aattgcgctg ggtgacgctg cagaagacgc atcggcatta
     1501 tccgatctga gccaccctgg aaatcggaga cagagcgtgt cgcccgtttt tttacgggtt
     1561 tgacggccaa accgaacccg gtatcgttgc cgacaccatg aatcgatttg gacaaagacc
     1621 gctttgaaag aggcgcgttc agaaaatggc gaactcaacg ccgtgtttcc gaccggtggc
     1681 ggagaggtgg gagagctgat tcgcggtttc gactgggccg cgaccagcct gggtcccatt
     1741 tccgcatggc cgcagagcct gaagaccgcc accaacctgc tgctgctctc ccccgtgccg
     1801 atcgtcctcc tgtggggcga ggacggcatc atgatctata acgacgccta ttccgttttc
     1861 gccggcgccc gccatcccaa attgctggga tccaaggtgc gcgagggctg ggccgagatc
     1921 tccgacttca acgacaatgt gatgaaggtc ggcctggcgg gcaacacgct ggcctataag
     1981 gatcaggaac tcaccctcat tcgccgcggc cggccggaac cggtctggat ggatctcgat
     2041 tattcgccgg tcctcgatga aagcggccgt cccggcggcg tcatcgccat cgtggtggag
     2101 acgaccgaaa gggtgctcgc cgatcggcgc ctgcgcgaaa gcgagcagcg tttccgggcc
     2161 cttgccactg cgacctccga cgcggtctat cgcatgagcc cggactggaa ggagatgcgc
     2221 cagctcgatg gccgcggttt cctctccgac ctggaaagtc cgagcgtcgc ctggatggat
     2281 gcctatctgt tcgccgaaga ccagcctgcc gtgctggccg tcatcgagca ggcggtgcgg
     2341 acgagaggca ttttccagct cgaacatcgg gtccgccagg cggacggttc cgcgggatgg
     2401 acattctccc gtgccgttcc cgtgttcgat gaacagggcg agatcgtcga atggttcggc
     2461 acggcgaccg acgtcaccgg acggcgccgt gccgaagccc ggcgcgaggc gctggttcgt
     2521 ctgaccgaag agatccagaa actgaacaac ccggtcgaca tcgcctttgc cgcctcgcgg
     2581 atcctggggg agacgctggg cgtcagccgt gtcggctatg gcacggtcga ccccgcggac
     2641 gattccttcg tggtggcgcg ggagtggaac gcgccggggg tagagagcct cgccggcacg
     2701 ctcaatctgc gcgatttcgg cagcttcgtc gacgatctca agctggggaa acttatcgtc
     2761 atcagcgacg ccatgctcga cccgagaacg aaggacgcgg cggatgccct gattgcccgt
     2821 tacgcccgct cgttcgtcga tgtcgccgtg gtcgagcagg accaggcggt cgccgtcttc
     2881 tatgtcagcc acgccgaggc tcgcgactgg tcggcggaag agctcggcct gatccgcgag
     2941 gtggcggcca gaacccgcac cgccatcgaa cgccagcgtg ccgaacgcaa gttccaggac
     3001 ctcgccgaaa aactggaaag ccaggtcgag gagcggaccc gcgagcgcga ccgcgtctgg
     3061 cagaacacgc aggagctgag cgccgtcatc gaccgcgacg gcatcttcgt cgcggtcaat
     3121 ccggcgacga ccaagctgct cggctggacc gaagccgact tgatcggccg ttccctgttc
     3181 gatttcgtcc tgcccgacga tctggaggcc accggaggag cctttgagcg cgccaggcgc
     3241 gaggcgcttc cggccttcca gaaccgctat cgcaccaaag gtggcggctt tcgctggatc
     3301 tcctgggtgg cggcgcccga tcaggagctt atctttgcca gcgggcgcga catcaccgcc
     3361 gaaaaagaag cggccgatgc gctcgcggtt tcggaggacg ccctgcgcca ggcgcagaag
     3421 atggaggcgg tgggccagct gaccggcggt ctcgcccatg atttcaacaa tatcctggcg
     3481 gggatcagcg gcagcctcga gctgatgcag aggaagctca gccagggccg cgtcggcgat
     3541 ctcgatcgct atctcgccct cgcccatggt gccacgcggc gcgccgccgc gcttacgcac
     3601 cgcctgctgg ccttttcccg ccgccagacg ctcgacccca agccgaccaa tatcaatcgc
     3661 ctggttgcgg gaatggagga cctgatccgc cgatcggtcg gaccggagat cgacattggg
     3721 acggatgcgg ccgacgggtt gtggaacacg ctggtcgatg ccggccagct ggaaaacgcg
     3781 ctgctcaatc tttgcctcaa tgcgcgcgac gccatgcccg acggcggaat gttgatcatc
     3841 gaaaccgaca atcaccagtt cgacggacaa gcggcccgcg agcgcgatct gccgcccggg
     3901 caatatgtct cgctctgcgt ccgcgacacc ggtgccggca tgacgccgga cgtggtggcg
     3961 cgcgccttcg atcccttctt cacgaccaag ccgatcggcc agggaaccgg gctcggcctg
     4021 tcgatggtct atggcttcgc ccgccagtcc ggcggcctgg cgcgcatcga gaccgagctc
     4081 ggcaagggat cgaaggtctg catctatctg ccgcgccatc tcgccgaggt ggaagaagcc
     4141 gtctccgacg acgccgaggc gccgcgggcc gtcgcgggac acaccattct ggtcgtcgac
     4201 gacgagccgc tggtgcgttt gttcgtcacc gaggtgctcg gggatctcgg ctacaccacg
     4261 ctcgaagccg aggatgccca ggccggcctc gatattctgc gatcgacggc gcagatcgat
     4321 tttctggtca ccgatgtcgg cttgccgaac ggcatgaatg gccgccagct cgccgacgcc
     4381 gcgcgggaat tgcggccggg gctgaaggtg ctgttcataa cgggatatgc ggagaacgct
     4441 ctattgagcg aagggcatct cgggcgtggg atgcaggcca tgaccaagcc gttcacgatg
     4501 gaggaactgg cggtccggct gagggatagt ctcggggagg ggtgggcgtg aacttcaaag
     4561 gctcccatgg tatcagtaag cattgaagga atttttacct tctgatagca ctctactgaa
     4621 aatgaaacgc tccagaccca ccacgaacac aaaagatagg gatattcgcg aaccgctgat
     4681 ttcgtggatc gcctcgcggc atcctgctga tggctctaca ggcattattc aagaattcga
     4741 aatgccacgc ccgtcagcgc gcattgatct tgctgttgtt aacggcgaga tgagtggttt
     4801 tgagataaag agcgacgcgg acaccctcat tcgactgagg agtcaggtac ccgcattttc
     4861 aaggttcttt gacaaagtca gccttgtcac cacgcagagg catttgccct gcgcgcgtaa
     4921 aatgatccct gagtggtggg ggattatggt gttcaaacaa gaaggggcgt ttagagttgt
     4981 gcgctccgca aagcagaacc ggaatacaga tgtttcctcc ctgcttttcg cgctaacgaa
     5041 agcagagatt gttgaaatag cgcaacgtgc cggtctaaaa atgcggatat cgcaaaaaaa
     5101 agccgacatg accggttcgg tagttattga aatttcggag agcgacctat tcgtctatgc
     5161 gcgtgatatt attcgcaaac gaacggattc cgttattctt cagggttcat gacttcttga
     5221 gaaaatctga tctggcgatg aatatgcata ttgatctttg ctgcatccca aaatcgcccc
     5281 gattgtaggc ccgctatgtc gtcgtttgag gcgcgttcaa gcacggtgcc gccccagcac
     5341 tctggaaatc ccacgaaatc ttcatgatca cggatttctt gtgctccggt gatccagcct
     5401 tgggcatctt gcgagtttgc gtgcttataa atcagccaag cgtcgtctaa cggataggtg
     5461 acccttgcct tccagtcgcc tggcatgaag gtgttatttt tttttaagcg cctatgtccg
     5521 caatagtctc caaagagcag agcgaatccc tcgcttgctt gggaccagag tccccacgct
     5581 ccgttttcat aaaccctcac accatctcca tttggtggcg tagtaaagaa gtcgctcata
     5641 cacacagcgc ttacataagg cgcctgagat ggctcaattt cttcgaggat tcgtgcaatc
     5701 gcctgtcggg caaactcgac ccgctccgta accatctgac ggccttggcc gcaatccaaa
     5761 atgatgagaa gccgagaggc gctctctagg attgcaataa tctggccgat caatccaaaa
     5821 attgcaggat tagtgtcttg tgaaatgcgg actgcaatac attcggagcc accatcagaa
     5881 agtcggacgg cttgtctaag tacactatta gcttgagttg ctggatcggt aaactgaaga
     5941 acagggatcg cattaggaaa attggcgata agatttcgcc aagcgacgaa gccattctca
     6001 ggcgctaata aggcagtcat ggccgcattg taagcatctt gtaatgcctg cgtttgcgct
     6061 atccttacgt ggtccggatt gtttttctga acatatgctg gaggtgcgcg gtctttcgaa
     6121 aggtccagta tgaacggcct gtctccgatg gcccccgaaa tcgaatctat cgattcttga
     6181 agagatgcct cattttttgc ctgatttatc tcgaaaactg gaagaattgc ttctttgtct
     6241 tcatcggcaa gttcgcgata ggcgagatgc tccgcttcag agctttgaag gcatggaaag
     6301 tatgaatagt aagagaattc gacttgcgcc atcatcgccc tcaatcagca actatctagt
     6361 gtgttttata gaatttagca tctcttggga gaaatcaaaa ctgtcgttgt gtgtgggggg
     6421 ggggggg
//
LOCUS       DATGEM010000403         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702 k141_72,
            whole genome shotgun sequence.
ACCESSION   DATGEM010000403 DATGEM010000000
VERSION     DATGEM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..463
                     /locus_tag="VKQ27_12885"
     CDS             44..463
                     /locus_tag="VKQ27_12885"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLY89869.1"
                     /translation="MRIALETACVRTLCDRPERPSLDELKSIWLVAPKRRLRDGEQVC
                     ALDEAFHSTLVAATGNREMARCHQEVTERIRLLRRLDFTEEGRITATYEEHARILRAV
                     LQQRGDRAVQLLRSHIETSKAEVKKISLHKLYSARRR"
     gene            complement(493..1095)
                     /locus_tag="VKQ27_12890"
     CDS             complement(493..1095)
                     /locus_tag="VKQ27_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HLY89870.1"
                     /translation="MIDMQRDFIEPGGFGETLGNNVGLLAAIVPTVKRLLDACRKAGI
                     TIIHTREAHRPDLSDLPTAKLNRGNPKQRIGDPSAMGRILIAGEPGNDIVPGCAPLPG
                     EIVIDKPGKGAFYATALGDILRLRGTTHLLFAGVTTEVCVQTTMREANDRGYDSLLIE
                     DATESYFPEFKRAATEMIRAQGAIVGWTATCDQLAASLHK"
     gene            complement(1160..2764)
                     /locus_tag="VKQ27_12895"
     CDS             complement(1160..2764)
                     /locus_tag="VKQ27_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89871.1"
                     /translation="MSESEMMRFKPEHEIVLDHALEVEVSGMTKRFGAFTALDDVSMR
                     VAAGSFHALLGENGAGKSTLVKCLVGFYAPTAGQISVDGRERTINSPHDSHALGIGMV
                     YQHFTLVPSMTAAENLVMSRTDVPAIINWRQERARLAAFMDSVPFKVPLDQPVSQLAA
                     GEKQKLEILKQIYLDRRFMILDEPTSVLTPSEADEVLGMLRDMTRAQKVTILMITHKF
                     REVMAFADAASVLRRGKLTGEGRIADLNPARLAEMMVGSRDIPQAHGTKSGKAADEPR
                     LSVRNLVVEDDTGLPAVEDFSLDVRPGEIVGIAGVSGNGQRELVEALIGQRTPVAGSI
                     HVGGKPYAASRGEIRYHRAFSLPEEPLRNACVPGMSVAENMALRNFDQAPIATGIWLH
                     GSAMREQAQRWIVAFKVKTQGADAPIATLSGGNVQRAVLARELSESVSVLIVANPVFG
                     LDFAAVAEIHDRILAARNAGAAVLLVSEDLDELLELSDRLLVIFDGRIVHDTPTAQAD
                     IAVIGPCMAGHAGATAAGARGITEAH"
     gene            complement(2764..>3067)
                     /locus_tag="VKQ27_12900"
     CDS             complement(2764..>3067)
                     /locus_tag="VKQ27_12900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLY89872.1"
                     /translation="LPGEPVIDKPGKGSFYATNFDLVLHNRGIRNLILTGITTDVCVH
                     TTMRDANDRGYECLILSDCTAATDYGNHLAALKMVTMQGGVFGAVAESTAVIKAIA"
BASE COUNT          522 a         1000 c         1003 g          542 t
ORIGIN      
        1 cgttcgcgag ctggatttca ggcagttcga cgatctgtac gacttgcgca tcgcgcttga
       61 aaccgcgtgc gtgcggaccc tgtgcgatcg acccgagcgg ccgagcctcg atgaactgaa
      121 atcaatctgg ctggttgcgc cgaaacgcag gctgcgggac ggcgagcaag tgtgcgcgct
      181 ggacgaagcc tttcacagca cgctggtcgc cgccaccggc aaccgcgaga tggcgcgctg
      241 ccatcaggaa gtaaccgagc gcatccgcct gctgcgccgg ctcgatttca ccgaggaagg
      301 ccgcatcacc gccacctacg aggaacacgc gcgaatcctg cgcgccgtgc tgcaacagcg
      361 cggcgatcgc gccgtgcaac tgctgcgcag ccacatcgaa accagcaagg ccgaggtcaa
      421 gaaaatcagc ctgcacaagc tgtattcggc gcgacgccgc tagccagatc tcgatcggga
      481 accactcgag tttcacttgt gaagcgaggc ggcgagctgg tcgcaggtcg cggtccagcc
      541 gacgatggcg ccctgggcgc ggatcatctc cgtggcggcg cgcttgaatt cggggaaata
      601 gctttcggtg gcgtcttcga tcagcaatga atcgtagccg cggtcgttgg cttcgcgcat
      661 ggtggtctgc acgcagactt cggtggtcac gccggcgaac agcagatgcg tggtgccacg
      721 caggcgcagg atatctccga gcgcggtggc gtagaacgcg cccttgccag gcttgtcgat
      781 gacgatctcg cccggcagcg gcgcgcaacc gggcacgatg tcgttgccgg gctcgccggc
      841 gatgaggatg cgccccatcg cgctcggatc gccgatgcgc tgcttcggat tgccgcgatt
      901 gagcttggcg gtcggcaggt cggacaagtc gggccggtgc gcttcgcggg tatggatgat
      961 cgtgatgccg gccttgcggc aggcgtcgag cagcctcttt accgttggca cgatcgccgc
     1021 cagcaagccg acgttgttgc ccagcgtttc gccgaagccg ccgggctcga tgaaatcgcg
     1081 ttgcatgtcg atcacgatca gcgccgtctt cgccggggaa aactcgaagt cgtagggctt
     1141 tgcctttacg atcgccatgt caatgtgcct ccgtgatccc tctggcgccg gccgcagtag
     1201 cacccgcatg tcccgccatg caaggcccga tcacggcgat gtcggcctgc gcggtgggcg
     1261 tatcgtggac gatgcggccg tcgaaaatca ccagcagccg gtcggagagc tccagcagtt
     1321 catccagatc ttcgctgacc agcagcaccg ccgcgcccgc attgcgcgcc gcgagaatgc
     1381 ggtcgtggat ttccgcgacc gcggcgaagt ccagaccgaa caccggattg gcgacgatca
     1441 gcaccgacac gctttccgac agttcccgcg ccagcaccgc gcgctgcacg ttgccgccag
     1501 acaacgtggc gatcggtgca tcggcgccct gggtcttgac cttgaacgcg acgatccagc
     1561 gctgcgcctg ctcgcgcatg gcgctgccgt gcaaccagat accggtagcg atcggcgcct
     1621 ggtcgaagtt gcgcaaggcc atgttctccg cgacgctcat tcccggcacg caggcgttgc
     1681 gcagcggttc ttccggcagg ctgaaggcgc gatggtagcg gatctcaccg cgcgatgccg
     1741 catacggttt gccgccgaca tggatggaac ccgccaccgg cgtgcgctgg ccgatcagcg
     1801 cctccaccag ttcgcgctgg ccgttgccgg aaacgcccgc gatgccgacg atctcgccgg
     1861 gccgcacgtc gagcgagaaa tcctcgaccg ccggcaggcc ggtgtcgtct tcgacgacca
     1921 gattccggac ggacaggcgc ggctcgtcgg cggcctttcc ggacttggtg ccatgggctt
     1981 gcggaatgtc gcgcgagccg accatcattt cggcgagcct tgccggattc agatcggcga
     2041 tgcgcccttc cccggtcagc ttgccccggc gcagcaccga cgcggcatcg gcgaaggcca
     2101 tcacctcgcg gaacttgtgg gtgatcatca ggatagtgac tttctgcgcg cgcgtcatgt
     2161 cgcgcagcat gcccagcacc tcgtcggctt ccgatggcgt gagcaccgaa gtcggctcgt
     2221 cgaggatcat gaagcgccgg tcgaggtaga tctgcttcaa gatttccagc ttctgcttct
     2281 cgccggccgc gagttgcgaa accggctggt cgagcggcac cttgaacggc acggaatcca
     2341 tgaaggccgc gagccgcgcg cgctcctgcc gccagttgat gatggccggc acgtcggtac
     2401 gcgacatcac caggttttcc gcggcggtca tgctcggcac cagcgtgaaa tgctggtaca
     2461 ccatgccgat gccgagcgca tgcgaatcgt gcggggaatt gatggtgcgc tcgcgcccgt
     2521 caacggagat ctgccccgcg gtcggtgcgt agaaccccac caggcatttc accagcgtgc
     2581 tcttgcccgc gccgttctcg cccagcagcg catggaaaga gcccgccgcc acgcgcatcg
     2641 acacgtcgtc gagcgcggtg aaggcgccga agcgcttggt catgccggac acttccacct
     2701 cgagcgcatg gtcgagcacg atctcgtgct cgggcttgaa ccgcatcatt tccgattcgg
     2761 acatcaggcg atcgccttga tcacggcggt ggattccgca acggcgccga acacgccgcc
     2821 ctgcatcgtc accatcttca acgcggcgag atggttgccg tagtccgtgg ccgcagtgca
     2881 gtccgacagg atcaggcatt cgtagccgcg gtcgttggca tcgcgcatgg tcgtgtgcac
     2941 gcacacgtcg gtggtgatac cggtcaggat gagattgcgt atgccgcggt tgtgcagcac
     3001 caggtcgaag ttggtggcgt agaacgaacc cttgccgggt ttgtcgatga ccggctcgcc
     3061 gggcagc
//
LOCUS       DATGEM010000404         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1449196, whole genome shotgun sequence.
ACCESSION   DATGEM010000404 DATGEM010000000
VERSION     DATGEM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449196"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VKQ27_12905"
     CDS             complement(<1..414)
                     /locus_tag="VKQ27_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HLY89873.1"
                     /translation="MNTNLLRASCAVAALLMLVACDDKTVDPSTQYGKNPVLPAPQQY
                     VLPPMRIALAEGWRTGRTPAVANGLEVKAFATDFMHPRMVYSLPNGDVLVVETNGPKA
                     PIYRPKDFIEGKVKALSGSGAPAGNRIILLRDTKGT"
     gene            complement(411..839)
                     /locus_tag="VKQ27_12910"
     CDS             complement(411..839)
                     /locus_tag="VKQ27_12910"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HLY89874.1"
                     /translation="MTTEDPLPGPLVHPPLVAMAAALLIAPLATDLFYWQTQLFQWNN
                     FSIWLLTAGLILAAVAGLALLLDVARRRLPSVAWGRFGGFTAAAALGVLNAFIHSRDA
                     YTAVVPDGLQLSAAITVVLVSLGWRGWSLRRSASTLESHS"
     gene            complement(966..1586)
                     /locus_tag="VKQ27_12915"
     CDS             complement(966..1586)
                     /locus_tag="VKQ27_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89875.1"
                     /translation="MVEKSMMTSGLVLRPLTPDSEIETDPDALIIVLFMAGDGPLQAE
                     FLTRNWQATVPRAMFLGIELNDMVWHVNLLALQRIVVEAAKAHGLQQEKVVLLGLGDA
                     GGLAIELVMQGVLTALGVIAYDIPILEEHGPLRRGAARIRLVQHHSDADPDNTRFYAL
                     IREMQRQNIDVEAVILPEDACLAMRAGSRFLVQLLANTDTPPGVPP"
     gene            complement(1794..2111)
                     /locus_tag="VKQ27_12920"
     CDS             complement(1794..2111)
                     /locus_tag="VKQ27_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLY89876.1"
                     /translation="MYSSIKASLLATSLSLAVALTSNCAYARTEWTDGGEDAQIAVAI
                     RAELVRQSGPQANHIRFQVLDHVVYLHGNVSTPREGLEEAEIIGGVQGVKKVVNLTWT
                     FNG"
     gene            complement(2333..3229)
                     /locus_tag="VKQ27_12925"
     CDS             complement(2333..3229)
                     /locus_tag="VKQ27_12925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY89877.1"
                     /translation="MIDLNLARVFVTIYETGSVSGAAKRLHVSQPSVSYALSRLRHLL
                     GEPLFTRSREGMTPTVFGTQLYRRFRAAVAEIEDAVASTRSFDPLSSTRRFRLALSDS
                     GEASLLPQILTTLGRLAPNVELEVIEVDIARLDEWLKAGKIDAAICKRCVPAAPTDTS
                     CAVVFTEPYACLVSQRHPRVTTSLSMKQYVAERHVAVMPDSGNHLIESRLRELGHERK
                     IGLRVPHFSALPHIVAASELLVTLPSRMAHLFASQAHNRALPLPFSVPKVQLALYWYE
                     HGGDIIALRWFCETLKDCLADL"
     gene            3420..4733
                     /locus_tag="VKQ27_12930"
     CDS             3420..4733
                     /locus_tag="VKQ27_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLY89878.1"
                     /translation="MTLKSIAAIAIGFSSVAYADDTGATLSGITLYGTVDVGVTYQTH
                     GVPENAYAPQGTEYLVSKSSNASTFALAENGMGNSKIGLKGVELLEDGWSALFKVETG
                     FNPASGQVSDSLRSIAGQNGVPLTQQTASADSSRAGQAFNQDAFVGITHARWGTLTFG
                     RQTTLQTDEISVYDPQNGAYAFSMLGYSGSGSGSGATEDLRLDNMVKYGVGLGPLHVG
                     LMYQFAGTLGRNDDAIAADIGGDYEGLSVDAVATHKKGGVTVSALTAAQVALEPAESL
                     AATVSDNTSYSFLARYSLDTIKVYGGYDYIRYANPSSPLPAGTQDIGGYDLSVVSNTA
                     YTKNKILQLAWVGARYSLTPTVELAGAWYRSAQNSFRGNGCNNTSAVSCSGQTNAVSL
                     TVDWHMSKRFDTYVGAMYSQAVGGMASGYLHNNTVDPTAGLRFTF"
BASE COUNT          972 a         1403 c         1497 g          921 t
ORIGIN      
        1 ggtacctttg gtatcgcgca gcaggatgat gcggttgccg gccggcgcgc ccgagccgga
       61 aagggccttc accttgccct cgatgaagtc cttcgggcga tagatgggcg ccttgggccc
      121 gttggtctcg accaccagca catcgccgtt gggcaaggaa tagaccatgc gggggtgcat
      181 gaagtccgtc gcgaaggcct tcacctcgag cccattcgcc acggccggag tccggcccgt
      241 gcgccagccc tcggcgaggg cgatccgcat cggcggcagg acgtattgct gtggcgcggg
      301 cagcacggga tttttgccgt actgggtgct cggatccacc gtcttatcgt cgcaggcgac
      361 gagcatcaaa agggcggcga cggcgcaaga ggcgcgtagg agattggtgt tcatgagtga
      421 ctttccagtg tggaggcgga ccggcgcagg ctccagccgc gccagccgag actgaccagg
      481 acgacggtaa tcgccgcgga gagctggagc ccgtcgggaa ccaccgccgt ataggcgtcg
      541 cggctgtgga tgaaggcgtt cagcaccccg agcgccgccg ccgccgtaaa gccgccgaag
      601 cggccccagg cgacggaggg gagccgccgt cgcgcgacat ccagcaaaag cgcgagcccg
      661 gcgacggccg ccaggatcag cccggcggtc agcagccaga tcgagaaatt gttccactgg
      721 aaaagctgcg tctgccagta aaaaaggtcg gtcgccaagg gcgcgatcag gagggccgcg
      781 gccatcgcca cgagcggggg atggacaagg gggccgggaa gggggtcttc ggttgtcata
      841 tacggttctc ctcaatgcca gagggaggta ggaagcgtcg tgacgagggc cgcgacgacg
      901 ccgtcaagtg caatgcccag ggctgcgaaa ccgtggatcc ggtgatcgtg actgtgcggc
      961 gcgggtcagg gcggcacgcc ggggggcgtg tccgtatttg ccagcagctg gaccaggaag
     1021 cggcttcccg cccgcattgc caggcaagcg tcttccggca ggatgacggc ctcgacgtcg
     1081 atgttttgcc gctgcatttc tcggatcaac gcatagaaac gggtattgtc cggatcggca
     1141 tcgctgtgat gctgaaccag ccgaatcctc gcggcgcccc gccgaagcgg accgtgttcc
     1201 tccaggatcg gaatgtcgta ggcgataacg ccgagcgcgg tcagaacacc ttgcatcacc
     1261 agttcgatgg ccaggcctcc ggcgtctccc agacccagca gcaccacctt ttcctgctgc
     1321 aggccgtgcg ccttggcggc ctcgaccaca atacgctgca acgccagcag attgacgtgc
     1381 cataccatgt cgttcaattc gatcccgagg aacatggcgc gcggtacggt cgcctgccag
     1441 tttcgcgtca aaaattcggc ctgcaagggg ccgtcgccag ccatgaaaag gactataatc
     1501 agggcgtcgg gatccgtttc gatctcgctg tcgggcgtta gcggccttaa aaccaatcca
     1561 cttgtcatca tcgacttttc aaccaagccc cggtgcggcc gggtactgac ataagctttt
     1621 ccgcatcaga actaaaacag attattcgga tgttattaag cgaaattctt gctgaattct
     1681 tgctggattt tgacgcgccg acgggggcgg cgaatcgcga ggtgcagcag aaaacgcctt
     1741 gcggcccagc tcgtcgcctt gccgcaatgc accctggacg gtaccaggga aggtcagccg
     1801 ttgaaggtcc aggtcagatt gacgaccttc ttgacgccct ggacgccgcc aatgatttcc
     1861 gcctcttcaa gcccctccct gggggtggag acgttgccgt gaaggtagac cacgtggtcg
     1921 agaacctgga agcggatgtg attggcctgc ggacccgatt ggcgcaccaa ttcggcgcgg
     1981 atcgcgacgg cgatttgcgc gtcctcgccg ccatccgtcc attcggtccg cgcataggcg
     2041 caattggagg tcaatgcgac ggcgagagag agtgatgtcg ccagtagtga agccttaatt
     2101 gagctataca tttagctgtg tccaaatatt aaaggtataa cctctgatga agaggtggat
     2161 atccccccca atactggatg atgttcaatt cacatatcgc catatttatt atagcgatga
     2221 catcgttgtg cgtaatgtcg tggagtttgg ctgatttttc tagtaaattc gctgtcactt
     2281 tttcacggcg cattgatgcc gaggtcgggc aagcggtcag gcggcgaccc tgctagagat
     2341 cggcgaggca atccttcagc gtttcgcaga accatcgcaa ggcgatgatg tcgccgccat
     2401 gctcatacca gtagagcgcg agttgcacct tcggaacact gaatggcagg gggagcgcgc
     2461 ggttgtgtgc ctgggaggca aacagatgcg ccatgcggga cggcagtgtc accagaagtt
     2521 cgctggcggc gacaatgtgc ggcagagcac tgaaatgggg aacgcgcagg ccgatcttac
     2581 gctcatgccc cagctcgcgc aggcggcttt cgataaggtg gttaccgctg tcgggcatga
     2641 cggccacatg ccgttccgcg acgtattgct tcatcgacaa gctcgtcgtc actctggggt
     2701 gtctctggct gaccagacag gcatagggct cggtgaagac gacggcgcag gatgtgtccg
     2761 tgggggcggc ggggacgcag cgcttgcaaa tggcggcatc gatcttgccg gccttcagcc
     2821 attcgtccag cctggcgata tccacctcga tgacctcaag ttcgacgttt ggcgccaacc
     2881 ttcccagcgt cgtcaagatt tgcggcagaa gggatgcctc gccgctatcc gagagcgcca
     2941 ggcgaaaacg gcgcgtcgag gatagagggt cgaagctgcg cgtgctggct accgcgtcct
     3001 cgatctccgc gacggcggcg cggaacctgc gatagagctg agttccgaag acggtcggcg
     3061 tcatgccttc acgagatcgc gtgaacaggg gttcccccag caaatgccgc aggcgggaaa
     3121 gagcgtaact gacggatggc tgactgacgt gaagccgctt ggccgcgccg ctgacgctgc
     3181 cggtctcgta tattgttaca aaaacgcgtg cgagattcag atcgatcata tctcccccca
     3241 aatataagac aggcttatat tatatgaaga actatttcga ttagatttat gtttcagagg
     3301 cttgcagact tcaccgatcg aagggatgcg ggccgttgtt tctggccgtc cgcagccctg
     3361 tgaatcaagg aagatcctcc tcatttccgc gaggaggctt ccagtgaggg gggccttacg
     3421 tgactttgaa atcaatcgcg gcgatcgcga tcggattctc cagcgttgcc tatgccgatg
     3481 ataccggcgc gacgctatcg gggatcacgc tctacgggac ggtcgatgtc ggcgtcacct
     3541 atcagaccca cggcgtaccc gaaaacgcct atgcccctca gggcaccgag tatctggttt
     3601 cgaaaagcag caatgcgtcg actttcgccc tggcggaaaa cggcatgggc aactccaaga
     3661 tcggcctcaa aggcgtcgaa ctcctggagg acgggtggtc ggccttgttc aaggtcgaaa
     3721 ccggcttcaa tccggcttcg ggccaggtgt cggacagcct cagatcgatc gccggacaaa
     3781 atggcgttcc cctgactcag cagacggcca gcgccgattc cagccgagcc ggacaagcct
     3841 tcaatcagga cgcttttgtc ggtatcacgc atgcccgctg ggggaccctg accttcggcc
     3901 gccagacgac gctgcagacc gatgaaatca gcgtctatga cccgcaaaac ggcgcttacg
     3961 ccttttcgat gctcggctac tccggcagcg gcagcggttc gggcgccacg gaagatctcc
     4021 ggctcgacaa tatggtgaaa tatggtgtcg gcctcggtcc tcttcatgtc ggcttgatgt
     4081 accagttcgc cggcacgctt ggccgcaacg acgacgccat cgcagccgat atcggcgggg
     4141 actatgaggg tctgtcggtc gatgccgttg cgacccacaa gaagggcggg gtgactgttt
     4201 ccgccctgac ggcagcccaa gtcgccctgg agccggccga gtctctggcg gcgaccgtgt
     4261 cggacaacac ctcctacagc ttcctggccc ggtacagcct cgacaccatc aaagtctacg
     4321 gcggctacga ttatatccgc tacgccaacc cgtcatcgcc cctgccggca ggcacgcagg
     4381 atattggcgg ctacgacctc agcgtggtca gcaatacggc ctacacgaaa aacaagattc
     4441 tgcagctcgc ctgggtcggc gcgagatatt ccctcacgcc gacggtcgag cttgccggtg
     4501 cctggtatcg cagcgcgcag aacagcttca ggggcaatgg ctgcaacaat acctccgcag
     4561 tttcctgtag cggccagacc aacgcagtgt cgctgacagt cgattggcat atgagcaagc
     4621 gcttcgacac ctatgtcggc gccatgtatt cgcaagcggt gggcggaatg gcctcgggtt
     4681 atctgcacaa taataccgtc gatccgacgg ctggcctgag attcaccttt tgatttccag
     4741 ggctggcgaa aaaaaggggg gcgaaatgcg ttatttttac aacatgaaga tcg
//
LOCUS       DATGEM010000405         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_934986, whole genome shotgun sequence.
ACCESSION   DATGEM010000405 DATGEM010000000
VERSION     DATGEM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934986"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VKQ27_12935"
     CDS             <1..799
                     /locus_tag="VKQ27_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89879.1"
                     /translation="VGFGIAILVAAVATYQSLLATQQAYYDRGHFAQVFATAKRVPEA
                     LAARLTAIPGIGVLETRVVEDVTLTLPGSAEPLTSHIVSLPDDREPLLNRLYLRQGRP
                     VGPNATNEVLVGEAFANANHLRSGGALTAILNGRLKTLRVAGIALSPEFVFATRPGDP
                     ISDDARYGVMWMARKGLAAAFDMEGAFNDISATLAPGANQAAVLDAIDRLLEPYAVVG
                     GVARRRSVVLSQRGETSAAVRQGLGAGDTVILFPTDAIADGVRVRPR"
     gene            796..1566
                     /locus_tag="VKQ27_12940"
     CDS             796..1566
                     /locus_tag="VKQ27_12940"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLY89880.1"
                     /translation="MSNIVGLTDVALFVAGFVSWSISTLAAGGGSVLIVAAVAVILRG
                     QAIAPVITMTSMLASPARMILLWRFIDWRVVRWYLPGATTGALLGGWTFAHIGTQWLQ
                     IVLALFLVSTAWQYRMGARPRSFSMRLPWFVPVSFVSGLISAVVGASGLLVNPFYLNY
                     GLMKEAMLATRAANSLAIQLVKVGAYVVFGVMQWRLARHGLVAGAGAITAIWLVNPWL
                     RYLSSFRFRQLAVLVMLFGGLLILWQQRQFLLSALGAG"
     gene            1613..2125
                     /locus_tag="VKQ27_12945"
     CDS             1613..2125
                     /locus_tag="VKQ27_12945"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLY89881.1"
                     /translation="MDHISEALLNFVGDHQAWAPAIVFALAFGESLAFISLLLPATAI
                     LLGVGGLIGAADIALWPIWCAAALGAAFGDWLSYWFGARYSYAIAHMWPLSRDPELLP
                     RGEAFFRKWGMLGVFFGRFFGPLRSITPLVAGICGMRQLPFQIANLASAVIWATGVLA
                     PGIVFMQWLL"
     gene            2225..2890
                     /locus_tag="VKQ27_12950"
     CDS             2225..2890
                     /locus_tag="VKQ27_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLY89882.1"
                     /translation="MSDLKEAEPAARVGVWDPFVRYGHWLLVIAFGVAYLTAEEEEGG
                     PELIHVWAGYIVGLIVVLRVLWGFVGPERARFQNFVYGPVTATRYLFDLLRGRARRYL
                     GHSPAGGAMVVLLLLSLAATVVTGLVAYGDQGKGPLAADGTPLMAVAYADEDESGRQR
                     GRDTGRNGENGEGAIGELHSALANITLAQVILHVIGVGLASAVHRENLVRAMIDGRKR
                     LDS"
     gene            complement(3130..3333)
                     /locus_tag="VKQ27_12955"
     CDS             complement(3130..3333)
                     /locus_tag="VKQ27_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HLY89883.1"
                     /translation="MTTDRAVLFFAGFMTLLSAVLVWLVSPWWLLLTALVGVNMIQAS
                     ITGFCPAAMLFKSLGTRPGVAFP"
     gene            complement(3379..>4200)
                     /locus_tag="VKQ27_12960"
     CDS             complement(3379..>4200)
                     /locus_tag="VKQ27_12960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HLY89884.1"
                     /translation="VQGASCFGPAYEFAYILDTELRKRRIRDRVKMTFVTPEPYIGHL
                     GLDGVGDTKSLMESEFRNRHISWITNAKVKQVSAGTMSVEEVDDSGSVRKTHELPFSF
                     SMLLPAFRGIPAIRGLDKLVNPRGFVLIDEHQRNPAYPEIFGIGVCVAIPPVGPTPVP
                     VGTPKTGFMIESMVTAAASNIGKLLRGETPSARATWNAVCLADFGDGGVAFVAQPQIP
                     PRNVNWSSKGTWVHLAKVAFEKYFLRKMRTGQSEPFFETFVLRRLDIHKLKEPAE"
BASE COUNT          667 a         1227 c         1439 g          867 t
ORIGIN      
        1 cgtcggtttc ggcatcgcca tcctggtggc tgccgtggcc acttatcaat cgctgctggc
       61 gacccagcag gcgtactacg atcggggcca ctttgcgcag gtgttcgcga ccgcgaagcg
      121 tgtccctgaa gcgctggccg cgcggctgac ggcgatcccc ggcatcggcg tgttggagac
      181 gcgcgtggtc gaggatgtga ccttaacgct gcctggctcc gccgagccgc tcacttcgca
      241 tatcgtgtcg ctgccggatg atcgcgaacc gctgctgaat cgcctgtatc tacgccaggg
      301 gcggccggtc gggccgaacg cgaccaacga ggtgctcgtc ggtgaggcgt tcgcgaacgc
      361 caaccatttg cggtccggcg gcgcgctgac agccatcctg aatggccggt tgaagacact
      421 gcgcgtcgcc ggcatcgcgc tgtcgccaga gttcgtgttc gcgacgaggc cgggcgatcc
      481 gatctcggac gacgcgcgct atggcgtcat gtggatggct cgcaagggcc tggccgcggc
      541 tttcgacatg gaaggtgcgt tcaacgatat aagcgcgacg ctcgcacctg gcgccaatca
      601 ggcggccgtg ctcgacgcga tcgaccgatt gctggagccc tatgcggtgg tcggtggcgt
      661 ggcacggcgg cgatcggtgg tgctttcaca gcgtggcgaa accagcgcgg cggtgcggca
      721 gggacttggc gccggtgaca cggtgatcct gtttccgacg gatgcgatcg ccgatggcgt
      781 gcgcgtgcgg ccacgatgag caatatcgtc gggcttactg atgtcgcgtt gttcgttgcc
      841 ggctttgtga gctggtcgat cagcacgctg gctgccggcg gcggcagcgt gctgattgtc
      901 gccgcggtcg cggtgatcct acgcggccag gcaatcgcac cggtgataac gatgaccagc
      961 atgcttgcca gtccggcacg catgatcctg ctgtggcggt tcatcgactg gcgcgtggta
     1021 cgctggtacc tgccgggggc gaccactggc gcgctgctcg gcggctggac attcgctcat
     1081 atcggcacgc agtggctgca gatcgtgctc gcgctgttcc tggtcagcac cgcctggcag
     1141 taccgcatgg gcgcacggcc tcgttctttc agcatgcggc tgccgtggtt cgtgccggtc
     1201 tcgttcgtct ctggcctgat ctcggcggtg gtgggggcca gcgggctgtt ggtgaacccg
     1261 ttctacctca actacgggct gatgaaggag gcgatgctgg ccacgagagc cgcgaactcc
     1321 ttggcaattc aactcgtgaa ggtcggtgcc tatgtggtgt tcggcgtcat gcaatggcgg
     1381 ctcgcgcggc acggattggt ggcaggtgcc ggggcaatca cggcaatctg gctggtcaac
     1441 ccgtggctgc ggtatctgtc gtcattccgc tttcgtcagc tcgccgtgct tgtcatgttg
     1501 ttcggggggt tgctgatcct ctggcaacag cgccagttcc tgctgtccgc gctgggtgcc
     1561 ggctaaggcg gccgcttcgc atgagaagcg taagcaaggc attgggctgg tggtggatca
     1621 catttcagaa gcgctgttga acttcgtcgg cgaccatcag gcgtgggctc cggcgatcgt
     1681 tttcgccctc gcattcggag agtcgcttgc attcatctct ttgcttttgc ccgcgaccgc
     1741 gatcctgctg ggcgtcggcg ggctgatcgg tgcggctgat atcgcgctct ggccgatctg
     1801 gtgcgcggcg gcacttggcg ctgcgttcgg cgattggttg tcctattggt tcggcgcccg
     1861 ctacagctat gccatcgcgc acatgtggcc attgtcgcgc gatcccgagc tgctgccgcg
     1921 gggagaggcc ttcttccgga aatggggtat gctcggcgtg ttcttcggcc gtttcttcgg
     1981 cccactgcgc tcaatcacgc cactcgtagc cggcatttgc ggaatgcggc agctgccgtt
     2041 tcagatcgcc aacctggcat ccgctgttat ttgggcgacg ggtgtgctgg cgccaggcat
     2101 cgtgttcatg cagtggctcc tctgacgcac gtcggcttgg gctggacgcc ggggatgcca
     2161 ctttgatgca ggtcaatgcg gattctgcaa ggtcgcgcga catgctaaac gcggaggact
     2221 ggctatgtcg gaccttaaag aagcggagcc ggcggcacgt gtcggtgtat gggatccttt
     2281 cgtgcgatac ggacactggc tgctcgtcat cgcatttggc gtcgcttacc tcacggctga
     2341 ggaagaagag ggtgggccgg agctcattca tgtatgggct ggctatattg tcggtctcat
     2401 cgtggttctg cgcgtgctgt ggggttttgt gggacctgag cgggctcgct ttcagaattt
     2461 cgtttatgga ccggtgactg cgacccggta tttattcgat ctgctgcgcg gccgtgcccg
     2521 ccgctatctc ggccacagcc cagccggcgg cgcgatggtg gtcctgctgc tgttgtcgct
     2581 tgcggcgacg gtcgtcactg gcctggtggc ttatggcgac caggggaagg gacctctcgc
     2641 tgctgacggc actccactga tggcggtggc ctatgctgat gaggatgaat caggccggca
     2701 acgtggccgt gacaccgggc gcaacgggga aaatggcgag ggcgctatcg gcgagctgca
     2761 cagcgcgctc gccaatatca cgctggcgca ggtgatcctg catgtgatcg gcgtcggcct
     2821 ggcgagtgcc gtgcaccggg agaacctggt ccgcgccatg attgacgggc gaaagcggct
     2881 ggatagctga tcgagagcgc gatgaaggcc aggcacttgg cgcggcacga gaccacgctt
     2941 gcggccttcc cccacagtga atagggcgac ggtcaacgat cgtatcgagc acattggggg
     3001 caggtgcgcg tcgacaatga gcgcagggct gttcgcatcg ctgaggagcg catcgccatg
     3061 ttggggcggt gcatggtcca gctatggacg tggatcgatc gctgtcggtc actaccgatt
     3121 tcggagcgct caggggaatg cgacgcccgg cctggtgccc aatgacttga agagcatggc
     3181 ggctggacag aagccggtta tgctcgcctg aatcatgttt acgccaacaa gagcggtgag
     3241 cagcagccac catgggctga ccagccaaac cagcacggcg ctgagcaacg tcatgaagcc
     3301 ggcgaagaac aggacggcac gatcggttgt catgtcaggc actcctagtc gaagcggttg
     3361 gggtccggcg gctgacgctc actctgccgg ctccttgagc ttgtgaatgt ccagccggcg
     3421 cagcacgaag gtttcgaaga acggctcgct ctggccggtc cgcatcttgc gcaagaaata
     3481 tttctcgaag gccaccttcg cgagatgcac ccaggtgcct ttggatgacc agttgacatt
     3541 gcgtggcggg atctgcggct gggcgacgaa ggccacgcca ccgtcaccga aatcggcaag
     3601 gcagaccgcg ttccatgtcg cgcgcgccga cggcgtttcg ccgcgcagca gcttgccgat
     3661 attggatgcc gcggcggtca ccattgattc gatcatgaag ccggtcttcg gcgtgccgac
     3721 cggcacaggc gtgggaccga cgggtggaat ggccacgcag acgccgatac cgaatatttc
     3781 cggataagct gggttccgct ggtgttcgtc gatcagcacg aagccgcgcg gattgaccag
     3841 tttgtcgagc ccgcgaatgg cggggatgcc acggaacgcc ggcagcaaca tcgagaagct
     3901 gaacggcagc tcgtgggtct tgcgcacgga gccactgtcg tccacttcct cgaccgacat
     3961 cgtgccggcc gatacctgct tcaccttcgc gttggtgatc cagctgatat ggcggttgcg
     4021 gaattcgctt tccatcagcg acttggtatc gccgacgcca tcgaggccga gatggccgat
     4081 atagggttcc ggcgtgacga acgtcatctt gacgcgatcg cgtatccggc gtttgcgcag
     4141 ctcggtatcc aggatgtagg cgaactcgta tgccgggccg aagcaggacg caccctgtac
//
LOCUS       DATGEM010000406         7417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_381379, whole genome shotgun sequence.
ACCESSION   DATGEM010000406 DATGEM010000000
VERSION     DATGEM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381379"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VKQ27_12965"
     CDS             complement(<1..456)
                     /locus_tag="VKQ27_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY89885.1"
                     /translation="MAADAHAAADFYGTARGAVAARLLRDRLALLWPDLSGQSVLGIG
                     FTAPYLRLWREQATRCIAVTPAQMGATRWPVHEPNLSCTAEEDALPFPDLTFDRVLLV
                     HGLEAAESARRMLRETWRVLKDDGRLLVVAPNRSGMWAYWESTPFGHGQP"
     gene            519..1250
                     /gene="gloB"
                     /locus_tag="VKQ27_12970"
     CDS             519..1250
                     /gene="gloB"
                     /locus_tag="VKQ27_12970"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HLY89886.1"
                     /translation="MTVTAQSVPILSDNYAWMLRDSGSGAVAIVDPADAKPIIAALEK
                     AGGRLDVILLTHHHADHIAGVDEVRAHFPGAKVVGASADAHRLPKLDLAVKEGDTVAF
                     GSASARVIDTPGHTRGQINYFFPDGEILLSGDTLFSLGCGRLIEGTAAEMFASLRKLA
                     ALPGGTLVCCGHEYTESNARFALSVDGGNAALKARTEEAHQQRASGVPTVPSTMTSEL
                     AANPFLRAPDVATFADLRAKKDSFR"
     gene            1247..1846
                     /locus_tag="VKQ27_12975"
     CDS             1247..1846
                     /locus_tag="VKQ27_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY89887.1"
                     /translation="MKLCFSPTSPYVRKVMACAITREIDARIERVTTNANASPPEFVA
                     INPLSKVPALVTDDGVALFDSPVICEYLDSIGEALPMFPDRGAARWRALKFQAMGDGI
                     LDASVPRRGEMTKPQDEGRAAWIARQKAAVDRTLGALEADPPHRHIDIGSITVACALG
                     YLDFRFAAQPWRAGRPQLAAWYEAFAQNPGIALTAPQDG"
     gene            complement(1889..3730)
                     /locus_tag="VKQ27_12980"
     CDS             complement(1889..3730)
                     /locus_tag="VKQ27_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HLY89888.1"
                     /translation="MRNAVRAVLMLAACMLASNSRAQVQQPGNLYEFGDSFQDAQYWC
                     TSFATPLRACSNYRDTPMRLGAISNYAFSLQNDYAVGSSASGPGNFVNTGYPPYPGSP
                     GSYPNMFGQIAEFQAQGRSISGNDLVTLSYAGNDVSVYGNPSTALARATIGYLTTDVR
                     ALIGLGARNFVLFGGVPFDRLLAGGPNLLQQQGVSLAADREYYTTLNAELPAAIRPFE
                     SASVHLRILDANTLYMRVLDTPAMYGFIFGDCAVVAGCASAPIAVQNQYAFYRGHPSD
                     AFALVIARYIDNLLTMPYQIAAEADLAQAAALAFHDTLTWRLDAEHLHFAGFGQNGTI
                     VPAVGRLAVFVSGDYAYADRSDRSNASGGNGNAGGLTAGAEYWATPNLLLGAAFSYSS
                     VGSTLNNGSGKINLNAYQFAGFASLGFPHWFVDGVLSGGVTGYTVKRPGVIDTLSGSP
                     QGSNIVGGVRGGYLFDVQHLQIGPIAGLTYVHVGVGSYTESGDIVLAQSVGSQKLDGL
                     TGSAGLQLRYPGLVYGHPIRSFVDLTAERDFLGDSRIITTTGLDAVSAVPVYTPVSSA
                     SGTYGRVAAGVEADLVQGVALGLMGGTSFARSSGNQGLVQVTLRAVF"
     gene            complement(4103..4396)
                     /locus_tag="VKQ27_12985"
     CDS             complement(4103..4396)
                     /locus_tag="VKQ27_12985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HLY89889.1"
                     /translation="MEMHVFARFHARAGCEDAVAAALRDVVPPTRDEPGCRVIDAYRA
                     TGDLRLFHIHSCWTDEAAFERHAQLPHTVRFLACVQPLIDHPLDVTRNYVLPC"
     gene            complement(4429..4632)
                     /locus_tag="VKQ27_12990"
     CDS             complement(4429..4632)
                     /locus_tag="VKQ27_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89890.1"
                     /translation="MSDATQIALAQQALSRATATLAVQAEVLADEFARGNLADYGGAD
                     ALRLFAAVVRAGGGNDLQVMGSA"
     gene            complement(4689..5471)
                     /locus_tag="VKQ27_12995"
     CDS             complement(4689..5471)
                     /locus_tag="VKQ27_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLY89891.1"
                     /translation="MSYQPEAAAATLHELRQRRGQVMPLPADVAPRNETEGAAVQRAL
                     AQRVGAASPPGFKIGATAKRMQEYLGLSGPAAGFMAMGGLYRSGATVKFGDFVRPGVE
                     CELVVRLGRDLPPAACTPEQAAAAVGELFAGIEIVENRYGDLLELGVPMLIADQMFHG
                     AAVLGAPGVEDWHTLDIGTLRGRLIIDGHQRDEGVGADLLGDPMRCLAWLAASSVAEA
                     FGGLKAGQVVMLGSVVPVIWLTGPASVTVDFSPLPPVQVTFT"
     gene            complement(5468..6211)
                     /gene="rlmB"
                     /locus_tag="VKQ27_13000"
     CDS             complement(5468..6211)
                     /gene="rlmB"
                     /locus_tag="VKQ27_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HLY89892.1"
                     /translation="MDHRPRAPDTPRGALWIYGLHAVAAALANPARRLRQLIVTEEAQ
                     QTLAQRQPQPWPVMPEQVDRGRLDHLLGRDIAHQGVALLADPLAIPSLQHALERPGPL
                     IVLDQVTDPRNVGAILRSAAAFGVAAVITQDRNAPEETGALAKAASGALETIPLLRAV
                     NIARTLIALKAANIWTVGLDGGGPPLSGPSLAGRRVALVLGAEGDGLRRLTRETCDEI
                     AGIQMPGKMDSLNVSAAASVALYELTRHA"
     gene            6515..7336
                     /gene="dapF"
                     /locus_tag="VKQ27_13005"
     CDS             6515..7336
                     /gene="dapF"
                     /locus_tag="VKQ27_13005"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLY89893.1"
                     /translation="MARFLKMHGCGNDFVVFDERAGSLGLTARRAAAIADRRTGVGCD
                     QFIVIEPPPSGSNADVFMRIRNPDGAEAGACGNATRCVASILAEETGRRAQVIHTIAG
                     NLPSQALPDGRICVDMGPAQLDWPDIPLARPMDTLHLDLRGDPAAVSMGNPHATFFVA
                     DVAATPVGEMGPQFEHDPLFPERANIGFAQILAPDRIRLRVWERGAGLTLACGSGACA
                     ALVNAHRRALTGRRAVVVVDGGELEIEWRADGNVLMTGPVATSFAGEIDLSAYPA"
BASE COUNT         1331 a         2489 c         2380 g         1217 t
ORIGIN      
        1 gggctggccg tggccgaacg gcgtgctttc ccaatacgcc cacataccgc tgcgattggg
       61 cgccaccacc agcaggcgcc catcgtcttt cagcacgcgc cacgtttccc gcagcatccg
      121 gcgggcgctt tcggcagctt ccagaccgtg caccaacagc acccggtcga acgtgaggtc
      181 ggggaacggc agggcgtctt cctcggcagt acaggagaga ttcggctcgt gaaccggcca
      241 tcgcgtggcg cccatctgcg ccggcgtcac cgcaatgcag cgggtcgcct gctcacgcca
      301 cagccgcaga tagggggcgg taaagccgat acccagcacc gactgcccgc tcaggtcggg
      361 ccatagcagg gcgaggcggt cgcgcagcag acgcgccgcc accgcccctc gggccgtgcc
      421 gtaaaagtcc gccgcagcgt gggcatcggc tgccatgaca cggtcctata acacggctgg
      481 cggttttcgg tggccgtcac tggtgctagg gtcgcgccat gacagtcaca gcacaatccg
      541 tccccatcct gtccgacaat tacgcctgga tgctgcgcga cagcggctcg ggcgcggttg
      601 ccatcgtcga tccggccgac gccaagccga ttatcgcggc actcgaaaag gcgggcggca
      661 ggctggatgt catcctgctg acgcaccacc acgccgatca cattgccggt gtcgacgagg
      721 tgcgcgcgca ttttccgggc gccaaggtcg tgggtgcgag cgctgacgcg caccgcctgc
      781 cgaagctcga cttggcagtg aaggaaggtg acacggtcgc gttcggcagc gccagcgccc
      841 gcgtgatcga cacccctggg cacacgcgcg gccagatcaa ctacttcttc cccgacggcg
      901 agatcctgct gtccggcgac accctgttca gcctcggatg cggccggctg atcgagggca
      961 ccgccgctga gatgttcgcc tcgctgcgca agctggcggc actgcccggc ggcaccctgg
     1021 tatgctgtgg gcatgaatat acagagagca atgcacggtt cgcgctgagt gtcgatggcg
     1081 gcaatgcggc gctgaaggct cggaccgagg aagcgcatca gcaacgcgca tccggcgtgc
     1141 ccaccgtccc ttccacgatg acgagcgaac tggccgccaa tccgttcctg cgcgccccgg
     1201 atgtcgcgac atttgccgat ctgcgcgcga agaaggacag tttccgttga aactttgctt
     1261 ctcgcccacc agtccttacg tccgcaaggt gatggcctgc gccatcaccc gcgagatcga
     1321 tgctcgcatt gagcgtgtga cgacgaacgc caatgcttcg ccccctgaat tcgtcgcaat
     1381 caatccgttg tcgaaggtgc cggcactcgt caccgacgac ggcgtggcgc tgttcgacag
     1441 tccggtgatc tgcgagtacc tcgacagcat cggcgaagcg ctgccgatgt tccccgaccg
     1501 cggtgccgca cggtggcggg cgctgaaatt tcaggccatg ggcgacggca tcctcgatgc
     1561 gtcggtcccg cggcgtggtg agatgacgaa gccgcaggac gagggccgcg cggcctggat
     1621 cgcacgacaa aaggcagcgg ttgatcgcac actcggtgcg ctggaggcgg acccgccgca
     1681 ccggcacatc gatatcggtt cgatcaccgt ggcttgcgcg ctgggctacc tggatttccg
     1741 cttcgccgcg cagccatggc gtgcggggcg gccgcaactg gcagcgtggt acgaggcgtt
     1801 tgcgcagaac cctggcatcg cattgaccgc accgcaggac ggttaggctt aggggtgatg
     1861 ccggcggcag agttggccga ctcttctcct agaaaactgc ccgcagcgtt acctgcacca
     1921 gcccctggtt gccgctgctg cgcgcaaagc tggtgccgcc cataaggccg agcgcgacgc
     1981 cctgcaccag gtctgcctcc acgccggcgg ccacgcgccc ataggtgccg gaggcgctcg
     2041 acaccggcgt gtaaaccggg acggccgaga cggcatccag gccggtcgtg gtgatgatcc
     2101 ggctatcgcc aaggaagtcg cgttccgcgg tgaggtcgac aaacgacctt atcggatgcc
     2161 cgtacaccag gccgggatac cgcaattgca ggccggctga gccggtcaga ccgtccagct
     2221 tctggctgcc gacggactgg gccagcacga tgtcgcctga ttcggtgtac gatcccacgc
     2281 cgacatggac ataggtcagg ccggcaatcg gcccgatttg gaggtgttga acgtcgaaca
     2341 gatagccgcc cctcactccg ccgacgatgt tgctgccctg aggactcccg gacagcgtat
     2401 cgataacgcc gggccgcttc accgtgtagc ctgtcacgcc accgctcagc acgccatcga
     2461 caaaccagtg ggggaagcca agggaagcga agcccgcgaa ctggtaggca ttgaggttga
     2521 tcttcccgct gccgttgttg agtgtcgaac cgacgctcga ataggaaaat gctgctccca
     2581 gcaacaggtt tggcgtggcc cagtattctg cgccggccgt caatccgccg gcgttgccat
     2641 tgccgcccga tgcgttgctg cggtcgctcc ggtcggcata ggcgtaatcg cctgagacga
     2701 acacggccag cctgccgacc gctggaacga ttgttccatt ctgcccgaag ccggcgaagt
     2761 ggagatgttc cgcgtccagg cgccacgtca gcgtgtcatg gaacgccagt gccgctgcct
     2821 gtgcgagatc cgcctcggcg gcgatctggt acggcatggt cagcaggttg tcgatgtagc
     2881 gggcgatcac cagggcaaag gcatccgacg gatggccacg atagaatgca tactggttct
     2941 gcacggcgat cggcgccgac gcgcaaccgg cgaccacggc acagtccccg aagatgaaac
     3001 cgtacatcgc cggggtatcc agcacccgca tatacagcgt gttcgcatcg agaatgcgca
     3061 gatgcacgga tgcgctttcg aacggcctga tggcagccgg aagctcggcg ttcagggtgg
     3121 tgtaatattc acggtcggcg gcaagggaga cgccttgctg ctgcagcagg tttggcccgc
     3181 cggccagcag gcgatcgaac ggcacgccgc cgaacaacac gaaattcctg gctcccagac
     3241 caatgagggc ccgcacatcg gtcgtcaggt agccgatggt cgcccgggcg agagcagtgc
     3301 tgggattgcc atacaccgac acgtcgttgc cggcatatga cagcgtcacc agatcgttgc
     3361 cgctgatgct tctgccctga gcctgaaatt ccgcgatttg gccgaacatg ttggggtagc
     3421 tgccggggct gcccgggtag ggcggatagc cggtattgac gaaattgcca ggaccagatg
     3481 cgctcgatcc cacggcgtag tcgttttgca gagagaatgc ataattcgat atggcgccta
     3541 gccgcatcgg cgtatcgcgg taattgctgc aagctcgaag cggggttgca aaactggtgc
     3601 accagtactg ggcatcctgg aaactatcgc cgaattcgta gagatttcct ggttgctgta
     3661 cctgcgcgcg ggagttcgac gccagcatgc aagccgccag catcaagacg gcacgaactg
     3721 cattgcgcat caggttgtcc cacgcgggaa ccgcctgcgt gtggccattt tggctgcagc
     3781 aatggaacat ggagccggga cctttctgct cgcgcggtcc cgtcagaaat cgtcgatccg
     3841 tactaaaaca gcgctaacgc ttgctgctta aggttcttgt ggcaacacca ctccagagcc
     3901 cccagcgccg ctggcctggt gcgcatcgga gttgatgaag tgccacgcta ccgtcgcatg
     3961 aaccttgttg cgagcatgcc gccaagcggt gacttcctcg atcagcatcg gcttttctgg
     4021 gatgcgacat cgagatgcgg ctgagttccg gcctggcccc aatggattca gagatcagcg
     4081 gcgaaatcca tgacgcacgg cactagcacg gcagcacata gttgcgggtc acatccaacg
     4141 gatggtcgat cagcggctgc acgcatgcga gaaagcgcac ggtgtgcggc aactgtgcgt
     4201 gacgctcgaa ggcagcttcg tccgtccagc acgagtggat atggaacagc cgcaaatcgc
     4261 ccgttgctcg ataagcatcg atcacgcgac agccaggctc gtcgcgggtt ggcggaacga
     4321 catcacgcaa ggcagcggca accgcgtcct cgcagccagc ccgcgcatgg aagcgggcga
     4381 acacgtgcat ttccattgtc ccctcccagt gcaagcctca gtgaaccgct acgctgatcc
     4441 catcacttgc agatcattcc caccgccagc cctgaccacc gcggcgaaca gccgcagcgc
     4501 atccgcaccg ccataatcgg ccaggttgcc gcgcgcaaat tcgtcagcca gcacttcggc
     4561 ctgcaccgca agggttgcgg tggcgcggct cagtgcctgc tgagcaagcg caatctgcgt
     4621 ggcgtctgac aacgcattcc aatcggatgg catacgtctg agcatacgcc gaccaggtta
     4681 ccagaacatt aagtgaacgt cacctgtacc ggcggcagcg gcgagaaatc cacggtcacg
     4741 ctcgccggcc cggtcagcca tatcaccggc accacactgc cgagcatgac cacctgcccg
     4801 gccttcaggc caccgaacgc ctccgcaacc gaagacgcag cgagccacgc caggcacctc
     4861 atcggatcac ccagcagatc ggcgccgaca ccttcgtcgc gctggtgccc atcaatgatc
     4921 agccgccctc gcagcgtccc aatgtcgagc gtgtgccagt cctccacgcc gggcgcgccc
     4981 agcacagcgg cgccgtggaa catctgatcg gcgatcagca tcggcacgcc aagttccaac
     5041 agatcgccat agcggttctc cacgatctcg atacccgcaa acaactcgcc cacggcagcg
     5101 gcggcctgct caggcgtgca cgctgctggt ggcaggtcac ggccaagccg caccaccaac
     5161 tcgcattcaa cgccgggccg gacgaaatcg ccgaacttca cggttgcccc gctccgatac
     5221 aggccaccca ttgccatgaa gcccgcggcc ggaccgctca gcccaaggta ctcctgcatg
     5281 cgcttcgcag tggcgccgat cttgaagccg ggtggcgatg cggcgccgac gcgctgagca
     5341 agggcgcgct gcacggcggc gccttcggtc tcattgcgcg gggccacgtc ggccggcaga
     5401 ggcatgacct ggccgcggcg ctgacgcagt tcatgcaacg tggcggcggc cgcctcgggc
     5461 tggtagctca cgcatgcctc gtcagttcat agagtgccac cgacgccgca gcggacacat
     5521 tgaggctgtc catcttgccc ggcatctgga tgccagcgat ttcgtcacag gtttcgcggg
     5581 tgagccggcg cagcccatcg ccctcggcac cgaggacaag cgcaacccgc cggcccgcaa
     5641 gcgatgggcc ggacagcggc ggcccaccgc catccaggcc gacggtccag atattggctg
     5701 ctttcagggc gatcagcgtg cgggcgatgt tgacggcacg cagcagggga atggtctcca
     5761 gcgcgcccga tgcggccttc gccagtgccc cggtctcctc gggcgcattg cggtcctgcg
     5821 tgatcacagc cgcgacgcca aacgccgccg ccgaacgcag gatggcgccg acattgcgcg
     5881 ggtcggtgac ctgatccagc acgatcagcg ggcccggccg ctcgagcgca tgctgcaggc
     5941 tgggtatggc aagcggatcg gccagcagcg cgaccccttg gtgagcgatg tcacggccca
     6001 gcaaatggtc gaggcgtccg cgatccacct gctccggcat caccggccaa ggctgcggct
     6061 gacgctgcgc cagcgtctgc tgcgcctcct cggtcacaat gagctgccgc agccggcgcg
     6121 ccggattggc cagcgccgcg gctaccgcat gcaggccgta gatccacagc gcgccccgcg
     6181 gcgtgtctgg cgcacgcggc cggtgatcca gctggggacg atgctggcga tggaaaccgc
     6241 caccgccacc gccaccctgg ccttggcccg gtttgccatg gaacggctta cctggcgggc
     6301 gaggcccccg atgctgctgc ggagggccgc cggagcctcg ttcgggaccg cgatcggacg
     6361 gcgggcggcc ggagccaccg gaccaactgg aacggggagg tttcattcga gcggtataga
     6421 ccgggtgttc aggactgccc agttcgcgtt cccctgcccc cagcattgac ggcgcctctt
     6481 tcgccagcgc ccccgcggcg catataccca ttccatggcg cgcttcctga agatgcacgg
     6541 ctgcggcaac gacttcgtcg tattcgacga gcgcgcgggc tcgctcggcc tcaccgcgcg
     6601 gcgtgcggcg gcgatcgccg atcggcgcac cggcgttggc tgcgaccagt tcattgtcat
     6661 cgagccgccg ccatccggca gcaacgccga tgtattcatg cgcatccgca atcccgatgg
     6721 cgctgaagcg ggcgcctgcg gcaacgccac ccgctgcgtc gccagtattc tggccgagga
     6781 gacgggccgg cgcgcgcagg tgatccacac cattgccggc aacctgccat cgcaagcact
     6841 gccggacggc cggatctgcg tcgatatggg accggcacag ctggactggc cggacatccc
     6901 cctcgcgcgg ccgatggaca cgctgcatct cgatctgcgc ggtgatccgg ccgcggtcag
     6961 catgggaaat ccgcacgcga cgttcttcgt ggctgatgtc gccgcgacac cggtgggcga
     7021 gatgggaccg cagttcgaac acgatccact gttccccgag cgtgccaata tcggctttgc
     7081 gcagatcctg gcacccgatc ggatcaggct gcgtgtctgg gaacgtggcg ccggcctgac
     7141 cctggcctgc ggctccggcg cgtgcgccgc gctggtgaat gcgcaccgcc gcgccctgac
     7201 ggggcgccgg gcggtcgtcg tcgtggacgg cggcgaactg gagatcgaat ggcgcgcgga
     7261 tgggaacgtg ctgatgaccg gcccggtcgc aacatctttc gcgggcgaga tcgatctgtc
     7321 ggcctatccg gcatgaccgt cgacatcctc accttcggtt gccggctgaa cgcctatgag
     7381 agcgaagtga tgcgcgggca cgccaccgcg ctgagcg
//
LOCUS       DATGEM010000407         7480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1149032, whole genome shotgun sequence.
ACCESSION   DATGEM010000407 DATGEM010000000
VERSION     DATGEM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149032"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2525)
                     /locus_tag="VKQ27_13010"
     CDS             complement(138..2525)
                     /locus_tag="VKQ27_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLY89894.1"
                     /translation="MATARTVETRRLASPTIRALIHQRSAELLGLLLGVIGLALLVAL
                     AFYDPRDPSLNTATSRHTTNLAGPVGAILADLLLQGFGMAGALPGVAMLAWAWRIGSR
                     RGVGSMAARLASLIAALPVLAAVLAAIPVPQKFAWPTVAGLGGAIGRLFGAAGLAAGR
                     GVLGPFGAVTMWVIGLALAFTLTLLALGLTASEWGTAWGFSRKAARFSVSGGRGAASL
                     MGHIHLPRLPLPRLWRRPDRPPEAGSIVRREPVTTVSRQAPPRDEPPPVVARAGRPPP
                     PRKPQQESLPLLEAGWRFPPLSLLKPPPARASSGPTEEALQANARLLETVLSDYGVQG
                     SIVEIRPGPVVTLYELEPAPGIRSARVIGLADDIARSLSVIAVRIATVAGRNVIGIEV
                     PNAKRETVFLSEILSSEELQKHGGRLTLALGKEINGAPQFADLARMPHLMIAGTTGSG
                     KSVGVNAMILSLLYRLSPDQCRLILIDPKMLELSMYEGIPHLMAPVVTEPAKAVTALK
                     WTVREMERRYRAMSQLGVRNLTGYNDRVAEARIKGEVVTRRVQTGFDPDTGRPTFEEQ
                     PLALEPLPLIVVVVDEMADLMMVAGKEIEAAVQRLAQMARAAGIHVIMATQRPSVDVI
                     TGTIKANFPTRISFQVISKFDSRTILGEQGAEQLLGMGDMLYMQGGGRITRVHGPFVS
                     DEEVEKVVEFLREQGEPAYVEDVTEAPDDEEAGAMLPGLSADGGGDKDLYGQAVGVVA
                     REGKASTSFIQRHLNIGYNRAAKLIEQMEKEGVVGPANHVGKREVLIRRVIDEED"
     gene            2636..3850
                     /locus_tag="VKQ27_13015"
     CDS             2636..3850
                     /locus_tag="VKQ27_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HLY89895.1"
                     /translation="MSDRLTAEVCVVGAGPVGGALACRLAAAGIATVVVDRAPLPPME
                     HPAFDGRAYAIAAGSRRLLEEAGVWDRLPEPVCPILDIRVSDGRLGRRASPLHLHFAH
                     RDTGEDAGPFGHMVEARSLRVALNGLLHALPALRVFAPAVAAVDRREDGATVRIADGP
                     VIDCRLVVAAEGRSSPLRRAAGIPVTSLPYRQTGIVVAVSHERPHNNTAVEHFLPSGP
                     FAQLPMCGSPGVAPHVSAIVWTEHTPIAQRMLALEDAPFVREIARRLGDHLGSIGALG
                     RRWSYPLAAMHAHRYYDTRLVLVGDAAHTIHPIAGQGLNLGFRDAIALADLLIEASGH
                     SEDVGAPDLLRRYQRRRRPDNLLMLAATDGLDRLFSSNNPLVRLARDIGIATVHRTPP
                     LKRLFMRQAMGA"
     gene            complement(3872..5905)
                     /locus_tag="VKQ27_13020"
     CDS             complement(3872..5905)
                     /locus_tag="VKQ27_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLY89896.1"
                     /translation="MRDRKSAMSFVSDRTIGSKVAAGFAVVLLILAVSSVLSWLSFGR
                     VGEAARKYAQLVAVSDIYQQIDTTLVQYRGQVREYIFSNDEATADAAVKDGEALRKLI
                     ANGLALAVDPARRALLEDMVKQEQSYTSGFIRSRSSNLEQDKVSAELESIGNQLAASL
                     SGIVESATKAGNAEAVSMAADTRRISLVTRLDAAKRLNAHDEAAGKSVEKGVGDLRQT
                     LARLDTATKGTEINAAVSPLTASIDRWQSAFQRAVNLDTEQLEMLNGSMRQAGDTLIA
                     DAQKATDANLAEQATVEKETLAASSNGSNLTMLFGVAALVIGAALAWLIGHGISRPVV
                     RMCAVMRALADGDKTVEIPNIGRKDEIGQMADAVQIFKQGIIEADRQREANERQKAEA
                     ETERKAGMIRLADTFEASIKGVVNAVASQATDMQSAAQAMSHTAGSATEQATAVAAAV
                     EQASANVQTVATAAEELSASVLEIGRQVEQSSKIAGQAVVEADHTNDTVEGLDRTAQR
                     IGEVVQLIETIAGQTNLLALNATIEAARAGDAGKGFAVVASEVKSLASQTAKATEEIR
                     GQIGEIQGATSQAVQAIHTIGATIRQMNEIATTIASAVEEQGAATREIATNVHQAAQG
                     TSAIASNIEGVSRAAQETGAAASQVLGSAGELSRQSVTLRSDVEHFLSTVRAA"
     gene            6063..7034
                     /locus_tag="VKQ27_13025"
     CDS             6063..7034
                     /locus_tag="VKQ27_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLY89897.1"
                     /translation="MAPKILLLDNPLSAMDIARELAPSGFETIIARGGSPEFNAALPD
                     VEYIVGLGEVKMDDAFYKSAPNLKLVQLLSAGYDRVDIEAARRASVPVCNNGGANAIA
                     VAEHAILLMLAVSRRLTWLHEMVKSGRWRGNDVASVRLYELHARTLGIIGLGNIGKKV
                     ARLAQGFGMAVQYFDIARLSEDAADALGVKFRLFNEVLRTSDIVSLHVPLIPSTKHMM
                     GAAQFGLMKKTAYLINTCRGPVVNEPELHDALVNGTIAGAGLDVFDQEPPPANNPLFT
                     LDNVILTPHYAGPTWDNQSARFRNGFDNVQRVARGEKPLWVIPELRE"
     gene            7291..>7480
                     /locus_tag="VKQ27_13030"
     CDS             7291..>7480
                     /locus_tag="VKQ27_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY89898.1"
                     /translation="MGENRSDFAARLRQIMAEINVSRVMLSRELGVDKSVIGRWLGGI
                     NQPTGHNLTRLTDIIRRYR"
BASE COUNT         1242 a         2496 c         2370 g         1372 t
ORIGIN      
        1 ccgccagcaa cggccagggc atgcgcccag atgcggacca acgcgccgcc agttgacgag
       61 ggatcagacg gagcgcgtcg gccatggggg catgctaggc agaaagcgaa acgttggcga
      121 cgggcatcct tcgccggcta atcctcctcg tcgatcacgc gccggatcag tacttcccgc
      181 ttgccaacat ggttcgcagg gccaaccacg ccctccttct ccatctgctc gatcagcttc
      241 gcggcacgat tgtagccaat gttcaggtgc cgctggatga agctggtgct ggccttaccc
      301 tcgcgcgcca ctacccccac cgcctggcca tacaggtctt tgtcgccgcc gccatccgcg
      361 ctcaatccag gcagcatcgc gccggcttcc tcatcgtcgg gcgcctcggt cacgtcctcc
      421 acataggccg gctcgccctg ctcgcgcagg aactcgacga ccttctcgac ctcctcgtcc
      481 gacacgaatg gcccgtgcac gcgcgtgatc cgtccacccc cctgcatata cagcatgtcg
      541 cccatgccca ggagctgttc ggccccctgc tcgcccagga tcgtccgcga atcgaatttg
      601 gagatcacct ggaacgaaat gcgcgtcggg aagttcgcct tgatcgtgcc ggtgatcacg
      661 tccaccgacg gccgctgcgt cgccatgatc acgtggatgc cggcggcgcg tgccatctgc
      721 gccaggcgct gcaccgcggc ctcgatctcc tttccggcga ccatcatcag gtccgccatc
      781 tcgtccacca ccacgacgat cagtggcagc ggttccagcg ccagcggttg ctcctcgaat
      841 gtcggccgcc ccgtgtccgg atcgaatcca gtctgtaccc ggcgcgttac tacctcgccc
      901 ttgatgcgcg cctcggccac acggtcgttg tatcccgtga ggttacgcac gccgagctgg
      961 ctcatcgccc gatagcgccg ctccatctcg cgcaccgtcc atttcagcgc ggtcaccgcc
     1021 ttggccggct ctgtgacgac gggcgccatc aggtgcggga tgccctcgta catcgagagc
     1081 tccagcatct tcggatcgat caggatcagc cggcactgat cgggcgacag ccgatacagc
     1141 aatgacagga tcatcgcatt gacgcccacc gatttgcccg agccggtggt gcccgcgatc
     1201 atcagatgcg gcatgcgcgc caggtctgcg aattgcggcg cgccattgat ctccttgccg
     1261 agcgccagcg tcaggcgtcc gccgtgcttc tgcagttcct ccgatgacag gatctccgac
     1321 aggaacacag tctcgcgctt ggcgttcggc acctcgatgc cgatgacgtt gcggcctgcg
     1381 acggtggcga tacgtaccgc gatcacggac aggctgcgtg cgatatcgtc cgccaggcca
     1441 atgacgcggg cgctgcggat gcctggggct ggttccagct catacagcgt gacgacgggg
     1501 ccgggacgaa tctccactat gctaccctgc acaccgtagt cggacagcac cgtctcgagc
     1561 agtcgggcat tggcttgcaa cgcttcttct gtaggtccag aggatgcgcg cgccggcggc
     1621 ggcttcagca acgacaacgg tggaaagcgc cagcctgctt ccagcagcgg cagactttcc
     1681 tgctgcggct tccgcggcgg cgggggacgc ccggcacggg ccaccactgg cggcggttcg
     1741 tcgcgtggcg gtgcctgccg cgacacggtt gttaccggct cccggcggac gatgcttccg
     1801 gcttccggcg gccggtctgg acggcgccac agccgaggga gtggcagtcg tggcaggtgt
     1861 atgtggccca tcaggctggc agcgccgcgt ccgcctgaca cgctgaaccg agccgctttc
     1921 ctgctgaagc cccaggcggt gccccattca ctggctgtca ggcccagtgc cagcaaggtc
     1981 agtgtgaagg caagtgccag gccgatgacc cacatcgtca ccgccccgaa cgggccgagc
     2041 acgccacggc ctgcagcgag gccggcagca ccaaacagtc ggccgatcgc accacccagc
     2101 cccgcgacag tgggccaggc aaacttctgc ggcacaggaa tcgctgcgag caccgcagcc
     2161 agcacaggaa gagccgcaat cagcgaggcg agtcgcgctg ccatgctgcc cacgccccgg
     2221 cgggagccga ttcgccaggc ccaggcaagc atcgccaccc ccggcagcgc gcccgccatc
     2281 ccaaatccct gcagcagcag gtctgccagg atcgcgccca ccggtccggc cagattggtc
     2341 gtgtgacgtg aggtggccgt gttcagcgaa ggatcgcgtg ggtcgtaaaa agccagcgcc
     2401 accaacagcg ccagcccgat aacgccgagc agcagcccca acagctcggc cgaacgctgg
     2461 tggatcaacg ccctgatcgt gggtgaggcg agccggcgcg tctcaacggt cctggcagtg
     2521 gccatgcgcg tcccaagttc gtgtgatgcg ccaactatag gctggaactg cctgatagca
     2581 gaagcggtct ccgcacagct tgccgccggt gtggcgcccg ggccactgtg tgccgatgag
     2641 tgatcgtctg accgccgagg tctgtgtggt gggcgccggc ccggtcggtg gcgcactggc
     2701 ctgccggctg gccgcggcag ggatcgccac cgtcgtcgtt gaccgcgcac cgctgccgcc
     2761 gatggagcat ccggcgttcg atggccgcgc ctacgcgatc gctgccggct cccgccgatt
     2821 gctggaagaa gccggtgtct gggaccggct gccggaaccg gtatgtccga tcctcgacat
     2881 cagggtcagc gacggccggt tggggcgccg tgcctcgccg ctgcatctgc acttcgccca
     2941 ccgggacacc ggcgaggatg ccgggccgtt cgggcacatg gtcgaggcgc gcagcctgcg
     3001 cgtcgcattg aatggtctcc tgcatgcgct gcctgccttg cgcgtgttcg cgccagcggt
     3061 ggccgcggtc gatcggcgcg aggacggcgc cacggtgcgg atcgccgatg ggcccgtgat
     3121 cgactgcagg ctggtggtgg cagccgaggg gcgcagttca ccgctgcgcc gtgccgccgg
     3181 cattccggtg accagcctgc cttaccgaca gaccggcatc gtcgttgccg tcagccacga
     3241 gcgcccgcac aacaacaccg cggtggaaca cttcctgccg tccgggccgt tcgcgcagct
     3301 gccgatgtgc ggcagccctg gcgtcgcgcc gcacgtatct gcaattgtat ggacagagca
     3361 cacgccgatc gcgcagcgca tgctggctct cgaggacgcg cctttcgtgc gcgagatcgc
     3421 gcgccggctc ggtgaccacc tgggatcgat cggcgcattg gggcggcgat ggagctatcc
     3481 gctcgccgca atgcatgcgc atcgctacta cgataccagg ctcgtgcttg taggtgatgc
     3541 ggcgcacacg atccatccga tcgctgggca gggactgaac ctcgggttcc gcgatgcgat
     3601 cgcgctggct gaccttctca tcgaggcgtc aggccacagc gaggatgttg gcgcgccgga
     3661 cctgctgcgc cgctatcagc gccgtcggcg gccggacaac ctgctgatgc tggcggcgac
     3721 ggacgggctc gatcggctgt tcagcagcaa caatccgctg gtgcggctgg cgcgtgatat
     3781 cggcattgcc accgtgcatc ggaccccacc gctcaagcgg ctgttcatgc gtcaggcgat
     3841 gggggcgtga gcccttttgg ctccttcctc gctacgcggc gcggactgtg ctcaggaaat
     3901 gttccacatc gctgcgcaga gttaccgact ggcgtgacag ttcgcctgcc gaaccgagca
     3961 cctgactggc cgcggcgccg gtctcctgcg cggctcggct gacaccttcg atattgctcg
     4021 cgatcgcgct cgtgccctgc gccgcctgat gcacattcgt ggcaatctcc cgcgtggccg
     4081 cgccttgctc ctccacagcc gaggcaatcg tcgtggcaat ctcgttcatt tgccgaatag
     4141 tggcgccgat ggtgtggatt gcctgtacgg cctggctcgt tgcaccctgg atctcgccaa
     4201 tctgcccgcg gatctcttcg gtcgccttcg cggtctggct tgcgagcgac ttcacttcgc
     4261 tggcaaccac cgcgaagcct ttgccggcat cgccggcccg tgcggcttcg atcgtcgcat
     4321 tcagcgccag caggttggtc tggcccgcga tcgtctcgat caattgtacg acctcgccga
     4381 tacgctgcgc cgtgcgatcc agaccctcga cggtgtcatt ggtgtgatcc gcttcaacga
     4441 cggcttgccc ggctatcttc gaagactgtt cgacctggcg gccgatttcc aataccgacg
     4501 cggacagttc ttccgctgcc gtggccacgg tctgcacgtt ggcggacgcc tgctcgacag
     4561 ccgcggcgac ggccgtcgcc tgctcggttg ctgacccggc ggtatgggac attgcctggg
     4621 ccgcggactg catgtcagtg gcctgcgagg cgactgcatt gaccactccc ttgatgctcg
     4681 cctcgaatgt gtcggcgagc cgtatcatcc cggccttgcg ctcggtctcg gcctccgctt
     4741 tctgccgttc gttcgcttcg cgctggcggt cggcttcgat gataccttgc ttgaagatct
     4801 gcacggcgtc ggccatctgg ccaatctcgt ccttgcgccc gatattcggt atctcaacag
     4861 tcttgtcgcc gtcggcgagc gcgcgcatca ccgcgcacat ccgtaccaca gggcgcgaga
     4921 tgccgtgccc gatcagccat gcaagtgcgg caccgatcac cagcgccgcc acgccaaaca
     4981 gcatcgtcag gttgctgccg ttggagctgg cggccagtgt ttccttctcg acggttgcct
     5041 gttcggcgag attggcgtcc gtcgccttct gcgcatcggc gatcagtgta tcaccggcct
     5101 ggcgcatgga gccgttcagc atctcgagtt gctcggtgtc caaattgacg gcgcgctgga
     5161 acgcggactg ccaccggtcg atggatgcgg tgagaggaga aacggccgcg ttgatctcgg
     5221 tgcctttggt cgctgtgtcc aaccgggcca atgtctgcct caggtcgccc actcccttct
     5281 caactgattt ccccgccgcc tcatcgtgtg cgttgagccg cttggccgca tcgagccggg
     5341 tgacgaggct gatccgccgg gtatcggctg ccatagagac ggcttcggca tttcctgcct
     5401 tggtggcgct ttccactatc cctgaaagac tggcggccag ctggttgcca atcgattcga
     5461 gttcagctga aaccttgtcc tgctccaggt tggaactgcg cgagcggatg aagccgctgg
     5521 tataggattg ctcctgtttg accatgtcct cgagcaaagc tctacgtgct ggatcaacgg
     5581 cgagtgcgag gccatttgcg ataagtttac gaagcgcctc gccatccttg acggccgcat
     5641 cggcagtggc ctcatcattc gagaagatgt actcgcgaac ctggccccga tactgcacca
     5701 gggtcgtgtc gatttgttga tagatatccg atacggcgac cagttgggcg tatttcctgg
     5761 ctgcctcacc gacccggccg aatgagagcc aggagaggac cgaactgacc gcaaggatca
     5821 gcaggacgac ggcgaagccg gccgcaacct tgctgccgat ggtgcggtcg gaaacaaacg
     5881 acatggctga tttgcggtca cgcattgaat gcatccttca acgccgcagc aggacgattg
     5941 cagcagggca cgccggtcgc gccgtgctgc tggcggtgaa tatcggcgtg cgaggtgaag
     6001 aattggctaa taacaaccgg ggcagctatc gttccccgat gaaccaatcg aggaggaaat
     6061 gaatggcgcc caagatcctg ctgctggaca acccgctgag cgcgatggac atcgcgcgcg
     6121 aactggcgcc atccggcttc gagaccatca tcgcacgcgg cggcagcccg gaattcaatg
     6181 cggctctgcc cgacgtcgag tacatcgtcg gcctcggcga ggtgaagatg gacgatgcat
     6241 tctataagtc cgcgcccaac ctgaagctgg tccagttgct gagcgctggc tacgaccgcg
     6301 tcgatatcga ggcggcaagg cgcgccagcg ttcccgtgtg caacaacggc ggtgccaatg
     6361 ctatcgcggt ggccgagcat gcgatcctgc tgatgctcgc ggtgagccgc cggctgacct
     6421 ggctgcatga gatggtgaag tccggccgct ggcgcggcaa cgatgtggcc agtgtgcgtc
     6481 tgtacgagct gcacgcacgt acgctcggca tcatcggcct cggcaatatc ggtaagaaag
     6541 tcgcgcggct ggcgcagggc ttcggcatgg ccgtgcagta tttcgacatc gcccgcctga
     6601 gcgaggatgc ggccgacgcg ctcggcgtga agttcaggct gttcaacgag gtgctgcgca
     6661 cgtccgatat cgtgtcgctg cacgtgccgc tgatccccag cacgaaacac atgatgggtg
     6721 ccgcacagtt cggcctgatg aagaagaccg cctacctgat caacacctgc cgcggcccgg
     6781 tggtgaacga gccggaactg cacgatgcgc tggtgaatgg cacgatcgcc ggtgccggcc
     6841 tcgacgtgtt cgatcaggaa ccgccccccg ccaacaatcc gctgttcacc ctggacaacg
     6901 tgatcctgac gccgcactac gccggaccga cctgggacaa tcagagcgcg cggttccgca
     6961 acgggttcga caacgtgcag cgcgtggcgc gtggcgagaa gccgctatgg gtaatcccgg
     7021 agttgaggga gtaacggacg caccggtttg cgcgcagacg cgccgtttcg cacccactct
     7081 cacctactcg cacgcggcgt tacagcggta tgcacaccgc gaacacgccg ccggcagcga
     7141 tcccccagtc gctgtcacgg acaccaacgg gctggaatga gtgccccatc ctggaccagc
     7201 cggccgatgg aatgctggcc cgggtgtccg gcggcgtgtt cgaatcgttc cgaattgaac
     7261 taattgtgac agcccgataa gctgggcttt atgggggaaa atcgatcgga ctttgccgcc
     7321 cgcctgcggc aaattatggc tgagatcaat gtcagccgtg tcatgctgtc ccgcgaactc
     7381 ggcgtcgaca agtcggtcat tggcaggtgg ttgggcggga tcaatcagcc aacagggcac
     7441 aatctgacaa ggttgaccga catcatccgt cgttatcgtc
//
LOCUS       DATGEM010000408         9182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1474013, whole genome shotgun sequence.
ACCESSION   DATGEM010000408 DATGEM010000000
VERSION     DATGEM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474013"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..642)
                     /locus_tag="VKQ27_13035"
     CDS             complement(115..642)
                     /locus_tag="VKQ27_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HLY89899.1"
                     /translation="MRVFLNTDPNGWGSYPGSSTPVSPVLALVGFVGLCLLVGAVGGT
                     IVAGTSWSWFLSLNRPAGMVPAWLFAPVWVCLYSMIGVAGWLAWRRSISASALRLWGW
                     QLAANALWVPAFFGLHSLPLALAVSAVLLVLVAITMRAFATLNSLAAWLLAPYLAWTG
                     YATYLNAGFWWLNPN"
     gene            complement(709..1806)
                     /locus_tag="VKQ27_13040"
     CDS             complement(709..1806)
                     /locus_tag="VKQ27_13040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLY89900.1"
                     /translation="MKLADLPTPCLVLDRNILLRNIGAMARALSRHRVPLRPHLKTAK
                     SIDVARLATAGQPGGITVSTLAEAEYFSGHGIADILYAVGITPQKLDQIAKLNAAGAA
                     IIVITDDLDTASAIAAHRQPPRVLVEIDTGEGRGGVAPDDDLLLEIATRLGPHLAGVM
                     THAGHSYAGRSIDDMIRIAEAERAGVVRAAERLRAAGHAVPTVSMGSSPTALHAESLD
                     GVSEVRAGVYMFGDLFQAEIGTHDLDSIALTVLTSVIGRRPGRLLVDAGGLALSKDHS
                     TETAPKDFGYGLVLDIAGRRAFGDTVVRRAYQEHGVVEAEPGQHLDLPVGGKLRIAPN
                     HTCMTAAAHDRYYVVEGGDDVVAIWTRVNGW"
     gene            complement(1803..3326)
                     /locus_tag="VKQ27_13045"
     CDS             complement(1803..3326)
                     /locus_tag="VKQ27_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HLY89901.1"
                     /translation="MTTPRIALLGFSIECNRFAPVAIEADFAARTLIRGAEMVADARS
                     SAPHMLGELPGFIADMDAAGPWEPVPVLLAMAEPNGPVEQAFFDRMMIEWEAGLREAG
                     HIDGVYCVLHGAGLTTADHDPEGTLLALVRRIVGPDVAVVASYDLHANVSDANVELVN
                     AYIGYRTNPHLDMRERGAESAQVLRKLLGGTQTHLARIRLPIVPPTVSMLTGKDAHER
                     PYGELIDLGQQRMHEAPYTGRVLNVSVMGGFAYADTPFNGLTAVVTATDADAARELAR
                     EIAQAGWARRERFRATLTSIEEATALARDTARPLIFADVADNPGGGGRGNTMWIIEAF
                     HKAGVQGALVGVIHDPSLAAEAHCLGEGAALTARFNRDGGDEFSKPFSARAVVRSLHD
                     GNMRGRRGIYANNTLALGPCAALQLDGITVVVISNRVQCADPIFFEAFGLDIAAARVV
                     VVKSRGHFRGGFDEFFRHEQVIEVDAPGLTSPILSRFPWKHMPRPVLPIDASAEWSA"
     gene            complement(3323..4444)
                     /locus_tag="VKQ27_13050"
     CDS             complement(3323..4444)
                     /locus_tag="VKQ27_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLY89902.1"
                     /translation="MEHAYLDPRTGETFPLDQPRWCGSGHAPLLLTPLPGLRRAQIDT
                     SVRSLWRYRAALPLQPAEAISMGEGCTPLIARTLDGAPALLKCEWMMPTGSFKDRGAS
                     VMLSLLREQGVRAVLEDSSGNGGAAVSAYAAAGGLRATIMAPASTSPAKTVQMRAHGA
                     TVELIPGTRQATADAAVARSETIFYASHNWHPFFLQGTKTLAYELWEDLGFRAPDNII
                     TPCGAGSNVLGCEIGFSELLRAGEVEAMPRIFAAQPANCGPIAAAFLGEEKPEIKPTI
                     AEGTAIAQPIRLAEVLGTLRETQGGAVMLSEDEIAAATLDLAGTGIYVEPTSGQAAAA
                     FRKLLAAGTITAEQTTVVVLTGSGLKATPRIGELLGVAL"
     gene            4554..5663
                     /locus_tag="VKQ27_13055"
     CDS             4554..5663
                     /locus_tag="VKQ27_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLY89903.1"
                     /translation="MIDTERSVTSLACELVSLDSRSFVSNLPVADRVAAALSGFDIEN
                     LDYTDAAGVAKRAIVAHRGPNGGIALSGHMDTVPDTGWQDDPWSARVENGILHGLGST
                     DMKGPVAAAIIAAQALPENIAVTLLITTDEETTKQGARLIAEKSQLVRQVKPAAIIVA
                     EPTTLAPVRGHRSHIAFTCVATGVQAHSSTGKGYNANWDLVPFLMEMKSIYERLRTDP
                     ALQDVNYDPPFSDFNVIIDNHGAAMNVTVPKATARIKFRYSAKIDPTPVLQAVYLAAE
                     RHRIQVSEAREGFPPELPADHALVRLCVDTTGQPAATAPYGTDASELQAIAPCVVLGP
                     GHISEAHTPTEKVRVADLVAAVPVFMRLAERCAAA"
     gene            complement(5667..7565)
                     /locus_tag="VKQ27_13060"
     CDS             complement(5667..7565)
                     /locus_tag="VKQ27_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89904.1"
                     /translation="MNPSLWPDLTHLPVWAVVVCIAGLCIGIGSWVGARRAETALVAG
                     WGVAGAATAVVGTLLMSGLGWVMLALAVAGLAGLVRIVIGSTTMRSAMLARVVLLALP
                     LAICIEGMDITGWDDFSHWLPNLTYLSLHGHFPTLVQPSTSVHAAYPYALALPGYAYF
                     LLFNRVPADAALIWNFLLMLAAGATVATVLLARLQANLPEHPLQSWATAAIGLLIAGL
                     AAPSFVAKIVFSNMADAPTASVLAVMGLLLFDWVIAARDRDAQGRTGAVWALAFAGVA
                     LIDLRQANAPLFAWLIFGCLLAGVTRPRWLDVRGLASLALIAVVPFAVWFLWNNYATT
                     QIPGGQFYILPLRGWRWAEFPTTLHSMLRVMLSKFGLFSVILLITIRAVLSFRRTDEL
                     GAPSRAVLITAAVVCVSNVAFLAFTYLAADFNKDEAAAAVSFWRYATQTGPIAVLGVA
                     AGLPLNWMRRIPAKPAAVALIVLALILPVATVKFYRFDLASSVPMLRSAGRATAESLP
                     PRAPITVVDLTGDGFAPLVVAYQIGQTDYARGLPARPFTIVANPAGITPAEAAKLHFE
                     VAPYLWLTEGAPELQPILGVHLAAGCSYLLQHDAGRFAIIKSWPLSRYLNTTQRSSWS
                     PATGAPCT"
     gene            complement(7562..8683)
                     /locus_tag="VKQ27_13065"
     CDS             complement(7562..8683)
                     /locus_tag="VKQ27_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLY89905.1"
                     /translation="MPLTDVGTSEALLAELATWVALETPTTDPSAVNRLMDLAEADLT
                     RAGAGLTRIPGRDGYGDNLIARTPGKGKPVLVAGHLDTVWSHGTLDTMPYQVNGQKAH
                     GPGIYDMKAGSFLAFHAVRSILRQKVATKRPIVLLLTPDEEVGSPTSREWIEREAADA
                     ACVLIPEPAARSGACVTARKGVGRFTMRVQGKGAHSGGAFEDGASAVVELARQIVRLH
                     GMVDLDRGITLNAAPIWGGSRPNVISPDAGCEIDLRVNSSADGEHMERALLESTALTP
                     GCRVTVEGGMNRPPYAEGPDILALYDHARGIAERVGVHLPRQHRGGGSDGNFTAAMGI
                     PTLDGLGCPGAGAHASHEHVLWQQLAPRAALMAGLLETL"
     gene            complement(8877..>9182)
                     /locus_tag="VKQ27_13070"
     CDS             complement(8877..>9182)
                     /locus_tag="VKQ27_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89906.1"
                     /translation="DIRKCKSDKYDGEAAFDDNKTTSMGGPIVRVDFAPDHTLIVIRV
                     VDEENCVFLRDNKDALLKHGVDPAKLVPYTVVEFEGHPHKTDKQKVLAGSVTVHDED"
BASE COUNT         1730 a         3098 c         2888 g         1466 t
ORIGIN      
        1 gggcaccgcc gccaatcgaa ttgaacctct gcgcctgagc gtacggcgca gccgcaacaa
       61 gcaccaggcc caaagaggcc agctgaatcc agaatcgcat atctcgaatg taactcaatt
      121 cgggttcaac caccagaacc ccgcgttcaa ataagttgcg tagccggtcc aggccaggta
      181 gggcgccagc agccatgccg ccaacgaatt taacgtcgcg aaggcacgca tagtgatggc
      241 aaccagcacc agcagcaccg cactgacggc cagcgcgagg ggcaggctgt gcaacccgaa
      301 aaaagctggc acccagagcg cattggcagc gagctgccag ccccacagcc gcagcgcgct
      361 ggcggaaatg ctgcgacgcc aggccagcca acccgccaca ccgatcatgg aataaagaca
      421 gacccagact ggagcgaaca gccacgccgg caccatacca gccggccggt tcagcgacag
      481 aaaccaactc caggacgttc cagcgacgat agtgccgccc acggcgccga ccaacaggca
      541 caacccaacg aacccgacca gcgcaaggac tggtgagacc ggggtcgagg atcccggata
      601 ggatccccat ccgttgggat cggtattgag gaagacacgc atcctttcgt tcgctcaggg
      661 aaaatgtagg ccctgacaaa cgtaatcgga agtgttttcc cggccgctct accaaccgtt
      721 gacccgggtc cagatggcga cgacatcgtc gcctccctcc accacgtaat agcggtcatg
      781 cgccgcagca gtcatgcagg tgtggttcgg tgcgatgcgc aattttccgc cgaccggcaa
      841 gtccagatgc tggccaggtt ccgcctcgac tacaccgtgt tcctgatagg cgcgccgcac
      901 gaccgtgtcg ccgaacgcac gtcgcccggc gatatccagc accaacccat agccaaaatc
      961 ctttggtgcc gtctccgtac tgtgatcctt cgacagcgcc agcccgccgg catccaccaa
     1021 cagacgccca ggacgacggc cgatgacgct cgtgagcacg gtcagggcaa tcgaatcgag
     1081 atcgtgcgtt ccgatctccg cctggaacag gtcaccgaac atgtagacgc cggcacggac
     1141 ttcgctgacg ccatcaaggc tttccgcatg cagcgcagtg ggagaactgc ccatcgacac
     1201 ggtgggaacc gcatgtcccg ctgcgcgcag gcgctccgcc gcgcgcacga cgccggctct
     1261 ttccgcctcc gcgatgcgga tcatgtcgtc aatgctgcgc ccggcgtagg agtggccagc
     1321 atgcgtcatc acgccggcga gatgcggccc cagccgcgtg gcgatctcca gcagcaggtc
     1381 atcatccggc gccacgccgc cgcggccttc acccgtatcg atttcgacca gcacccgagg
     1441 tggctgcctg tgcgcagcga tcgcgctcgc ggtatccaga tcgtccgtga tcacgatgat
     1501 ggcagcgccc gccgcattga gctttgcgat ctggtccagt ttctgcggcg taatgccaac
     1561 cgcgtacaag atatcggcga tgccatgacc ggagaaatac tccgcctcgg ccagcgtcga
     1621 gacggtgatg ccgccgggct gcccagccgt tgccaaccgc gccacatcga tcgacttcgc
     1681 ggtcttcaga tgcggccgca gtggcacgcg gtgccgcgac agtgcccgcg ccatcgcgcc
     1741 gatgttgcgc agcagaatat tgcgatcgag aaccaggcac ggcgtcggca gatcggcgag
     1801 cttcatgcgc tccactccgc gcttgcgtcg atcggcagca ccggacgcgg catgtgcttc
     1861 caggggaaac gcgacaggat cgggctggtg aggccaggcg catccacctc gatcacctgc
     1921 tcatggcgga agaactcatc aaagccgccg cggaaatgcc cgcgcgactt cacgaccacg
     1981 acgcgggccg ccgcgatgtc cagcccgaac gcctcaaaga atatgggatc ggcgcattgc
     2041 acgcggttcg agataaccac gacggtgatc ccgtcgagtt gcagcgcggc gcacggtccc
     2101 agcgccaagg tgttgttggc gtagatcccc cgccgcccgc gcatattgcc gtcgtgcaga
     2161 ctccgcacga cggcccgcgc gctgaacggc ttcgagaatt catcgccccc atcacggttg
     2221 aagcgagcgg tgagcgcggc gccttcgccc aggcaatgtg cctctgccgc cagcgatggg
     2281 tcgtggatca cgccgactag cgcgccctgt acgccggcct tatggaaggc ctcgatgatc
     2341 cacatagtgt tgccacggcc gccaccgccc ggattgtccg ccacatcggc aaaaatcagc
     2401 gggcgcgccg tatcgcgggc aagcgcggtt gcctcctcga tcgacgtcag cgtcgcgcgg
     2461 aaccgctccc gccgtgccca gcccgcctgg gcgatctcgc gtgcaagctc ccgtgctgca
     2521 tccgcgtcgg tcgcagtgac taccgcggtc agcccgttga acggcgtatc cgcgtaagca
     2581 aagcccccca tcaccgacac attcagcacc cgcccggtgt acggcgcttc atgcatgcgc
     2641 tgctgcccga gatcgatcag ctcgccatat ggccgttcgt gcgcatcctt gccggtgagc
     2701 atgctgacag tcggcggtac gatcggcagc cgtatgcgcg ccaggtgcgt ctgcgttcca
     2761 ccgagcagct tgcgcagcac ctgcgccgac tcggcacctc gttcgcgcat gtcgagatgc
     2821 gggttggtgc gatagccgat ataggcattc accagctcga cattcgcatc cgacacgttg
     2881 gcgtgcaggt cgtagctggc gacgacagcg acgtccgggc caacaatgcg tcgcaccaac
     2941 gcaagcaatg tgccttccgg gtcgtgatcc gccgtggtca ggccggcgcc atgcagcacg
     3001 cagtacacgc catcgatatg cccggcctcg cgcaagcccg cttcccattc gatcatcatg
     3061 cgatcgaaga acgcctgctc gaccggcccg ttcggttctg ccatcgccaa aaggactggc
     3121 accggttccc acggccccgc agcatccatg tcggcaatga agcccggcag ctcgcccagc
     3181 atatggggcg ctgaagagcg agcatccgcc accatctcgg cgccgcggat cagcgtgcgc
     3241 gcggcaaagt cagcttcgat cgcaaccggt gcgaagcgat tgcactcaat cgagaagccg
     3301 agcaaagcga tccgaggagt cgtcacagtg caactcccag cagctctccg atgcgaggcg
     3361 tcgccttcag tccgctgccg gtcagcacta ccactgttgt ctgctccgcc gttatggtac
     3421 ccgcggcgag caacttgcgg aacgcggctg ccgcctgacc ggacgttggc tccacataaa
     3481 tgccggtgcc tgccagatcg agcgtcgcgg ccgcgatttc gtcctcgctc agcatcaccg
     3541 caccgccttg cgtctcccgc agcgtgccga gaacctcagc caaccggatc ggctgggcaa
     3601 tcgcggtacc ttcggcgatc gtcggtttga tctccggctt ctcctcgcca aggaacgccg
     3661 ccgcgatcgg tccgcaattc gcaggttggg ccgcgaagat acgcggcatc gcttcgacct
     3721 cgccggcccg cagcaactcg gaaaatccga tctcgcagcc cagcacgttc gatcccgcgc
     3781 cacatggcgt gatgatgttg tccggcgcgc ggaagcccag gtcctcccac agttcataag
     3841 ccagcgtctt ggtgccttgc aggaagaacg gatgccagtt gtggctggca taaaatatgg
     3901 tctcggaacg cgcgaccgcc gcgtcagcgg tggcctggcg cgtgccaggt atcagctcca
     3961 ccgtggcgcc atgtgcacgc atctgcaccg tcttcgccgg gctggttgaa gcgggcgcca
     4021 tgatcgtcgc acgcaacccg ccggctgcgg cataggccga cacagcagcg ccgccattgc
     4081 cggaactgtc ctccagcacc gcgcgcacac cttgctcgcg caacagcgag agcatcacac
     4141 tggcgcctcg gtccttgaag ctgccggtcg gcatcatcca ttcgcacttc agcaacgcgg
     4201 gcgcaccatc cagcgtgcgt gcgatcagcg gcgtgcaccc ttcccccata ctgatcgcct
     4261 ctgccggctg cagcggcagc gcggcacgat agcgccacag gctgcggacc gatgtgtcga
     4321 tctgggcgcg gcgaagacca ggcaggggcg tcagcagcag cggcgcatgg cccgatccac
     4381 accagcgagg ctgatcgagc gggaacgttt cgccagtgcg aggatcaaga taggcgtgct
     4441 ccatgcgcgc agtctgcgcg gctgcggccc gagcctcaag gggagtgcta tcttgcgccg
     4501 ccgtcgcgat ccaggcgaag gtcccgcgca caagatgtcc ccggagcaat ggcgtgattg
     4561 acacagaacg atcggtaacc agcctcgcct gtgaactggt atccttggac agtcgcagct
     4621 tcgtctccaa tctcccggtc gcggatcgcg tcgcggcggc gctctctggc ttcgacatcg
     4681 agaacctgga ttacaccgat gctgccggcg tggcgaagcg cgccatcgtc gcgcatcgcg
     4741 gcccgaacgg tggcatcgca ctgtcgggcc acatggacac cgtgcccgat accggctggc
     4801 aggacgatcc ctggtcggcg cgcgtcgaga acggcatcct gcatggtctt ggcagcactg
     4861 acatgaaagg cccggtcgcc gctgcgatca tcgcggccca ggccctgccg gagaacatcg
     4921 cggtcaccct gctgatcacc acggatgagg aaaccaccaa gcaaggcgcg cgcctgatcg
     4981 cggagaagtc gcaactcgtt cgccaggtga agcccgcggc aatcatcgtc gcggagccca
     5041 ccacgctcgc gccggtgcgc ggccatcgca gtcacatcgc attcacctgc gtcgccaccg
     5101 gcgtacaggc gcacagcagc acaggcaaag gatacaacgc gaactgggac ctcgtgccct
     5161 tcctgatgga aatgaagtcg atctacgagc gcttgcgcac cgacccggcg ttgcaggacg
     5221 tcaattacga tccgccgttc tccgacttca acgtcatcat cgacaaccac ggcgcggcga
     5281 tgaacgtcac cgtgcccaaa gccactgcgc gcatcaaatt ccgctattca gccaagatcg
     5341 atccgacacc tgtgttgcag gctgtgtatt tggcggccga acgtcatcgc atccaggtca
     5401 gcgaggcgcg cgagggtttt cctccggaac tgcctgccga tcatgcgctc gtgaggctgt
     5461 gcgtggatac gaccggccag ccggcagcga ccgcacccta tggcaccgat gcatccgaat
     5521 tgcaggccat tgcgccatgc gtggtacttg ggccggggca catctccgag gcgcataccc
     5581 cgacagagaa ggtgcgcgtc gccgacctgg ttgccgccgt tccggtgttc atgcgcctgg
     5641 cggaacgctg cgctgccgcc tgatggctac gtgcaggggg cgccggttgc cggagaccaa
     5701 ctgctgcgct gcgtggtgtt gaggtatcgg ctgagtggcc agctcttgat gatggcaaag
     5761 cgaccagcgt catgctgcag cagataactg catccggccg ccaggtggac gccgaggatg
     5821 ggctgaagct ccggcgctcc ttcggtcagc cacagatagg gggccacctc gaaatgcagc
     5881 ttggcagcct cggccggagt gatccctgcc ggattggcga cgatggtaaa gggacgcgct
     5941 ggcaggccac gggcgtagtc ggtctggccg atctgatacg cgaccaccaa gggcgcgaag
     6001 ccgtcgccgg taagatcgac cacagtgatc ggtgctctcg gcggcaacga ttcagcggtc
     6061 gcccggcccg cactgcgaag catggggacc gacgaggcga gatcgaaccg atagaacttc
     6121 accgtcgcga ccggcaggat cagtgccagc acgatcagag ccaccgcggc aggcttcgcc
     6181 gggatgcgcc gcatccagtt cagcggcagg ccggccgcaa ccccgagcac ggcgatgggc
     6241 cccgtctgtg tcgcgtatcg ccagaacgag actgctgccg cggcttcgtc cttgttgaag
     6301 tccgccgcca gataggtgaa cgccaggaac gcgacgttgg acacgcagac gaccgctgca
     6361 gtgatcagca ccgcgcgtga cggcgcgccc agttcgtccg tgcgacggaa cgagaggacc
     6421 gcacgaattg tgatgagcag gatgacgctg aacagtccga acttgctcaa catcacgcgc
     6481 agcatgctgt gcagcgtggt cgggaattcg gcccagcgcc aaccgcgcaa aggcaggata
     6541 tagaactggc cgccagggat ttgcgtcgtt gcgtagttat tccacaagaa ccagacggcg
     6601 aacggcacca ctgcgatcag ggcaagcgac gccagtccgc gaacgtcgag ccagcgcggg
     6661 cgcgtgacac cggcgagcag gcaaccgaat atcagccagg caaacagtgg cgcattggcc
     6721 tggcgaagat ctatcagcgc gacaccggcg aatgccaatg cccagacggc gccggtccgc
     6781 ccctgggcat ccctatcacg cgccgcgatg acccagtcga aaagcagcag gcccatgacc
     6841 gccaacaccg aggcggtcgg cgcgtcggcc atgttgctga acacgatctt ggcgacgaag
     6901 cttggggcgg cgagcccagc tatcagcagg ccgatggcgg cagtggccca ggactgcaga
     6961 gggtgttctg gaaggttcgc ctgcagccgg gcgagcagca cggtcgccac cgtcgcgccg
     7021 gcggcaagca tcagcaggaa gttccagatc agggcggcat cggcgggcac gcggttgaac
     7081 agcaggaagt aggcatatcc cggcagagcc agcgcgtagg ggtaagccgc gtggaccgag
     7141 gtgctcggct ggaccagcgt cgggaagtgg ccgtgcaggc tcagataggt caggttcggc
     7201 agccagtggg aaaagtcgtc ccaaccggtg atgtccatcc cctcgatgca gatagcgagc
     7261 ggcagcgcca gcaagaccac gcgcgccagc atggcggatc gcatcgtggt gcttccgatc
     7321 acaatgcgga ccaggccggc gaggcccgcc actgcgagcg ccagcatgac ccagccgagg
     7381 cctgacatca gaagcgtgcc aacgaccgcc gttgccgcac cggcaacgcc ccagccagcg
     7441 accagcgccg tttcggcccg gcgggcgccg acccaggagc cgatgccgat gcagagccca
     7501 gcgatgcaga cgacaacagc ccagaccggc agatgtgtca ggtcgggcca gagcgagggg
     7561 ttcacagcgt ctccagcagc cccgccatca gcgccgcgcg cggcgcaagt tgctgccaca
     7621 ggacgtgctc gtggctggcg tgggcaccgg ctccgggaca gcccagccca tcaagcgtcg
     7681 gaatgcccat cgccgcggtg aagttgccgt ccgaaccgcc gccgcgatgc tgccggggaa
     7741 gatggacgcc gacgcgttcg gcgatgcccc gcgcatggtc gtagagcgcg aggatgtcgg
     7801 ggccttcggc ataaggcggc cggttcatgc caccttccac cgttacgcgg cagcctggcg
     7861 tcagggccgt gctctcgagg agagcgcgct ccatatgttc gccatcagcg ctggagttca
     7921 cccgtaggtc gatctcgcag cccgcatccg gcgagatcac gttcggccgg gacccgcccc
     7981 agatcggggc ggcattcaag gtgatgcctc gatcgagatc caccatgccg tgcaaccgca
     8041 caatctgtct ggcgagttca accaccgccg acgcgccatc ctcgaatgcg ccgcccgagt
     8101 gggcgccctt gccctgcacc cgcattgtga agcggccgac gcccttgcgg gcggtgacgc
     8161 aggcgccgct ccgggccgcc ggctcgggaa tcaggacaca ggcggcgtcg gcggcctcac
     8221 gctcgatcca ttcgcgcgac gtggggctgc cgacttcctc atcgggggtc agcagcagga
     8281 cgatggggcg ctttgtcgcg actttctggc gcaggatcga acgcaccgcg tggaaggcga
     8341 ggaagctgcc tgccttcatg tcgtagatgc ccggcccgtg cgccttctgg ccgttcacct
     8401 ggtagggcat cgtgtccagc gtgccgtggg accagaccgt gtcgagatgg cccgcgacga
     8461 gcaccggctt ccccttgccg ggcgtgcgcg cgatcaggtt gtcgccataa ccgtcgcgtc
     8521 caggaatgcg ggtgagcccg gcaccggcgc gggtgaggtc agcctcggca aggtccatca
     8581 ggcggttgac cgctgagggg tccgttgtgg gcgtctccaa cgcgacccag gtggcgagtt
     8641 ctgcgagaag ggcctcggag gttccaacgt cggtcagcgg catgcggcaa tccaggctcg
     8701 atgggagtgg agttgtgtgc gccaggatgg cagcattgtc cacgcagagg cggccaggct
     8761 caatcaagcc ttcgttgcgc tcttccagag tcagacgctt acgatcgcgc agttcaccgg
     8821 cgagattaag gcgtctccgg ccgcaccatg aagtcgtact tctgaggcgc gcgttatcag
     8881 tcctcgtcgt ggaccgtcac gctacccgct aacacctttt gcttgtcggt cttgtgtggg
     8941 tggccctcga actccaccac cgtgtacggc accagcttcg cgggatccac cccgtgcttc
     9001 agcaaggcgt ccttgttgtc gcggagaaac acgcagttct cctcgtccac gacacggata
     9061 acgatcagcg tgtggtccgg cgcgaaatcg acgcgcacga tcggccctcc catcgaagtc
     9121 gttttattgt cgtcgaacgc agcctcaccg tcgtatttgt cgctcttgca tttcctgata
     9181 tc
//
LOCUS       DATGEM010000409         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1048087, whole genome shotgun sequence.
ACCESSION   DATGEM010000409 DATGEM010000000
VERSION     DATGEM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048087"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VKQ27_13075"
     CDS             <1..216
                     /locus_tag="VKQ27_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89907.1"
                     /translation="SAGQRRRLALARLLLTRAMLWLLDEPTLGLDSASVERFGGLLAR
                     HRERGGIVVAATHLPLPMDGTAELALG"
     gene            245..910
                     /gene="ccmB"
                     /locus_tag="VKQ27_13080"
     CDS             245..910
                     /gene="ccmB"
                     /locus_tag="VKQ27_13080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLY89908.1"
                     /translation="MTTFLAVLIRDLRLSLRHGADTLAALLFFLIAAALFPLAVGPAP
                     ETLGRIAPGIVWVCALLAALLPLDRLFGADFEDGSLDQLLLSGLAPAGIALAKATSHW
                     LVTGLPLVLGAGPLAIMLRLPDVALPALLAGLLPGTLLLSLLGSTGAAIVLGARRGGV
                     LLPLLLLPLATPVLIFGTAAADAASGGLSPRPHLLLLTALLAAALPLCPLAAGAGLRS
                     AAE"
     gene            1134..3548
                     /locus_tag="VKQ27_13085"
     CDS             1134..3548
                     /locus_tag="VKQ27_13085"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HLY89909.1"
                     /translation="MLVTDDAVETLRRSIVEKLTYATGRDPLLAGDHDWFVATALALR
                     DHIVDRWLQSTRDDTANSRKRVYYLSLEFLPGRMLLDSLNNAGLTAPMRQALAKLGVN
                     LDRLRTLEPDPALGNGGLGRLAACFMESMASLGIPAHGYGIRYNHGLFQQVIRDGWQH
                     EFPENWLSLGNPWEFERADIRYNIGFGGRVDAPPFWDGRASSVWHPTETVFAMAYDMP
                     VLGWRGQHANTLRLWSARAPDPIALETFNSGDHVGALATRSRANAISQILYPSDETPA
                     GQELRLRQEYFFSSASLQDLLHRHIRQYGTLQNLSEKNAIQLNDTHPAIVVAELMRLL
                     VDEHFMPWSDAWRITTETISYTNHTLLPEALESWPVPLMERLLPRHMQIIYEINKQHL
                     DSLTDITDAPALHEVSLIDEHGERRVRMGTLAFIGSHKVNGVSALHTDLMRETVFQPL
                     HRLHPDRIVNKTNGITFRRWLHTANPRLTSLLCDAIGPQVLDDPLALERLAPLANDPA
                     LHDRLIAARRANKTALAALVAEKLDVTLDPDALVDVHIKRIHEYKRQLLNILETIALY
                     DAMRARPVRNWVPRVKLFAGRAAASYRRAKQIIKLIHDVARIVNDDPVVGDRLKVVFL
                     PDYNVSLAEMIIPAADVSEQISTAGMEASGTGNMKLALNGALTIGTLDGANLEILERV
                     GPENIFTFGLTAEQVEERRSTGMRPLSPTLERVLDVIDAGTFSPEEPDRYRSLVSDLR
                     EHDWFMVSADFEAYAETQRRVADRWCDRQGWWRSSVLNTANMGWFSSDRTIREYAREI
                     WGVPVA"
     gene            3545..>4458
                     /locus_tag="VKQ27_13090"
     CDS             3545..>4458
                     /locus_tag="VKQ27_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HLY89910.1"
                     /translation="MMVAPFPLIQVSGPPHDRGQQYGRQAAARIRKGIAHYSAQLEQL
                     NLDRAGVASLVRTYTPVIEAFDPTHLEEMRGIASGADVELADIVLLNARTEILKLAEK
                     PELRATLADGCTAVAVMPDATTDNILIHAQNWDWKAECAETAVVLHIRRNNGPDILTF
                     TEAGGLARSGMNSAGIAITANYLQSDRDYRDIGVPLALIRRKVLQQEHLALAMQAVHA
                     TQKSAANNMMVSGSRADGAAIAVDFECAPDEAFQVHPERGLLVHANHFVSAVALAKLR
                     DTGLASSPCSIYRDVRVRDLLRLQVGAI"
BASE COUNT          750 a         1476 c         1417 g          815 t
ORIGIN      
        1 tcggcagggc agaggcgcag gctggcactg gcgcggctgt tgctgacgcg cgcaatgcta
       61 tggctgctgg atgagccgac gctcgggctt gactcagcgt cggtcgagcg gttcggcggc
      121 ctgctggcgc ggcaccggga gcgtggtggg atcgtcgtgg cggcgacgca cctgccgctg
      181 ccaatggacg ggactgcgga actggcgctg ggatgagcgc ggcctccctt ccccactctg
      241 ctctatgaca accttcctcg cagtcctgat ccgcgacctc cgcctttccc tccggcatgg
      301 tgccgacact ctggcggcgc tgctcttctt cctgatcgcc gccgccctgt ttccgctcgc
      361 ggtcggtccc gccccggaaa cactcggacg catcgcccct ggcatcgtct gggtctgcgc
      421 cctcctggcc gcccttctgc cgctggaccg tctgttcggt gctgacttcg aggatggctc
      481 cctcgaccaa ctcctgctct caggcctggc gcccgccggg atcgccttgg ccaaggccac
      541 atctcactgg ctcgtgaccg gcctgcctct cgtcctgggc gccggtcctc tcgcgatcat
      601 gctccgcctg ccggatgtcg cgctgcccgc gctgcttgcc gggctgctgc ccgggacgct
      661 gctgctctcc ctgcttggaa gcaccggcgc cgccatcgtc ctcggcgcgc gacggggcgg
      721 tgtgctgctg cccctgctgc tgctgccgct cgccacgccg gtgctgatct tcggtaccgc
      781 ggcagccgac gccgcgtcgg gcggcctcag cccgcgcccg catctgctgc tgctcacggc
      841 gctgctggca gcggcattgc cgctctgtcc gctggccgcg ggagccggtt tgcgcagtgc
      901 agcagaataa atcggaacct tctgcatccg acgcgcgtat tctgcgccat aggaagatca
      961 ggcccgcgtc acgcgatttc ctttatttga aggttgcatg agagcgccac ggttcgttaa
     1021 cagactaccg tggggagacg cttttatgac acacactcat tggtcgcgcg gaggggctgt
     1081 ttcccatgga tctagttgag cccctttcga gtgcctggaa tgcaagcgcc gatatgttgg
     1141 taactgacga cgccgttgag acgctgcgcc gcagcattgt cgaaaagctc acctacgcga
     1201 ccggccgcga tccgttgctc gccggcgacc acgactggtt cgtggccacc gcgcttgcgc
     1261 tccgtgatca catcgtcgac cgatggttgc agagcacgcg cgacgacacc gcgaatagtc
     1321 gcaaacgcgt gtactatctc tctctagagt tcctgccggg ccgcatgttg ctcgactcgc
     1381 tgaacaacgc cggcctcacg gcgccgatgc gccaggcgtt ggcgaagctt ggcgtgaacc
     1441 tcgaccggct gcgcacgctg gagcctgatc cagcactggg caatggcggc ctcggccggc
     1501 tggcggcctg tttcatggaa agcatggcga gcctcggcat ccccgcgcat gggtacggta
     1561 tccgctacaa tcacggcctg ttccagcagg tgatccgcga cggctggcag cacgagtttc
     1621 cggagaactg gctgtcactc ggcaatccgt gggagttcga gcgcgccgac atcagatata
     1681 atatcggctt cggcggacgc gttgacgccc cacccttctg ggacgggcgt gcctcgtctg
     1741 tgtggcatcc caccgagact gtgttcgcca tggcctacga catgccggtg cttggctggc
     1801 gtggccagca cgccaacacg ctgcgcctgt ggtcggcgcg cgcgccagat ccgatcgcgc
     1861 tggagacatt caacagcggt gaccatgtcg gagcgctggc gacccgctca cgggcaaacg
     1921 cgatctcgca gatcctgtat cccagcgatg agacaccggc cgggcaggag ttgcgcctgc
     1981 gccaggagta cttcttctcg tccgcctcgc tccaggacct cctgcaccgt cacatccgcc
     2041 agtacggcac gctgcagaac ctgtcggaaa agaacgcgat ccagctcaac gatacccacc
     2101 ccgcgattgt cgtcgcggag ttgatgcggc ttctggtgga cgagcatttc atgccgtggt
     2161 ctgacgcgtg gcgtatcacc accgagacga tctcctacac gaaccatacg ctgctgcccg
     2221 aggcgctgga aagctggccg gtgccgctga tggagcggct gctgccgcgg cacatgcaga
     2281 taatctacga gatcaacaag cagcatctcg actcgctgac ggatatcacc gatgcacccg
     2341 cactgcacga agtctcgctg attgacgaac acggcgagcg ccgcgtgcgc atgggcacgc
     2401 tggcgttcat cggttcgcac aaggtgaatg gcgtctcagc gctgcacacc gacctgatgc
     2461 gcgagacggt gttccagcca ctgcatcgcc tgcatccgga ccgcatcgtc aacaagacca
     2521 acggcatcac tttccgccgc tggctgcata ccgccaaccc gcgccttact tcactgctat
     2581 gcgatgcgat cgggccccag gtgctggatg atcccttggc gctggaaagg ttggcgccgc
     2641 tggcgaatga tccggcgctg cacgaccggc tgatcgccgc acgtcgcgcc aacaagacgg
     2701 ctctggccgc gcttgtcgcc gagaaactgg acgtcacgct ggatcccgat gcgctggtgg
     2761 atgtgcacat caagcgcatc cacgagtaca agcgccaact gctgaacatt ctggaaacca
     2821 tcgcgctgta cgatgcgatg cgcgcgcgcc cggtgcgcaa ctgggtgccg cgcgtcaagc
     2881 tgttcgccgg gcgcgcggcc gccagctaca ggcgcgccaa gcagatcatc aagctgatcc
     2941 acgatgtggc gcgcatcgtg aacgatgatc cagtggtggg cgatcggctg aaagtcgtgt
     3001 tcctgcccga ttacaatgtc agcctggccg aaatgatcat tccggcggcc gacgtatcgg
     3061 agcagatctc caccgcaggc atggaggcgt cgggcaccgg caacatgaag ctggcgctga
     3121 atggcgcgct caccattggc acgctggatg gcgcgaacct cgaaatcctg gagcgcgtcg
     3181 gtcccgagaa catcttcacc ttcggcctga ccgccgagca ggttgaggaa cgccgcagta
     3241 ccggtatgcg gccgctgtcc ccgaccttgg agcgtgtgct cgacgtgatc gacgcgggca
     3301 cgttctcgcc cgaggaaccc gaccgttacc gcagcctcgt gagcgatctg cgcgagcatg
     3361 actggttcat ggtgtcggct gatttcgaag cctatgccga gacgcagcgc cgcgtcgcgg
     3421 accgctggtg cgaccggcaa gggtggtggc gctccagtgt gctcaacact gcgaatatgg
     3481 gctggttctc ctcggaccgg acgatccgcg aatatgcacg ggaaatctgg ggcgtgcccg
     3541 ttgcatgatg gtcgcgccgt tcccgctgat ccaggtttcc ggtccgccac atgaccgggg
     3601 gcaacagtat ggacgccagg cggcggcacg gatccgcaaa ggcatcgcgc actacagcgc
     3661 acagcttgaa cagctgaatc tcgatcgcgc cggcgtcgcg tcgctggtgc gtacgtatac
     3721 accggtcatc gaggcgttcg atcccacgca tctggaagag atgcgcggca tcgcctcggg
     3781 cgccgacgtc gagttggccg atatcgtatt gctgaacgcc cgaacggaaa tcctgaagct
     3841 ggccgagaag ccggagcttc gagcgacgct ggctgacggc tgcaccgctg ttgccgtgat
     3901 gccggacgcg accaccgaca acatcctgat ccatgcgcag aactgggatt ggaaggcaga
     3961 gtgcgccgag accgctgtcg tgctgcatat ccgccgcaac aacggaccgg atatcctcac
     4021 cttcactgaa gctggcgggc tggcgcggtc cggcatgaac agtgccggca ttgctatcac
     4081 ggcgaactac ctgcaatcgg accgcgacta ccgtgacatc ggcgttccac ttgcgctgat
     4141 ccggcgcaag gttctgcagc aggagcacct cgctctggcg atgcaggcgg tgcatgcgac
     4201 ccagaagtca gccgcaaaca acatgatggt aagcggcagc cgcgcggacg gcgcagcgat
     4261 cgccgtcgat ttcgaatgtg cgccggatga agcattccag gtgcatcccg agcgcggcct
     4321 gctggtgcac gccaaccact tcgtaagcgc ggtggcgctg gccaagctgc gcgataccgg
     4381 gctggcgagc tcgccttgca gcatctaccg cgatgtgcgg gtgcgtgatc tgctgcggct
     4441 gcaggtgggc gcgatcag
//
LOCUS       DATGEM010000410         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1374684, whole genome shotgun sequence.
ACCESSION   DATGEM010000410 DATGEM010000000
VERSION     DATGEM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374684"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VKQ27_13095"
     CDS             complement(<1..538)
                     /locus_tag="VKQ27_13095"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HLY89911.1"
                     /translation="MSRLDDRVRAASARYAVALGLRVAGALAKPVGPSALRALLDQLP
                     PHGRLRGSSPVAVPTAAELAEALDRGDIIAAFQPKVTLVERHVVGFQAMARWKHPPHG
                     PVHPDQFIPLAEHNGLIVPLTRRILEAALAACQHWRTRHPSCSVAVNISPMVLANPAL
                     PEEIEALLMQHALPPAALI"
     gene            complement(522..758)
                     /locus_tag="VKQ27_13100"
     CDS             complement(522..758)
                     /locus_tag="VKQ27_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY89912.1"
                     /translation="MVDDEPVQCLIVTRAMAAASFDAEAATSLDETAHRFASATFDVI
                     VLDLSLGGREGIILLRQIAAAASDPRGGADVTPG"
     gene            922..1788
                     /locus_tag="VKQ27_13105"
     CDS             922..1788
                     /locus_tag="VKQ27_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY89913.1"
                     /translation="MSLRLHLIVLGLLMLPASSAFAQAAVEDQYFITSDSVRLHYLEA
                     GPRTGHTIVFVPGWTMPAWIWQPQLQAFARRYHVVAFDPRGQGESDAPARGYEPNRRG
                     QDIAELVAHLEPVPVVLVGWSLGVLDSLAYLHTHGDQKVAGLVLVDNSVGEEPPPPPR
                     PPHRPAPVLPHAIAMHRFVMSMFHHPQSQAYLDRLTEATLRTPETASRALLAYPVPRT
                     YWRDALFSTSKPVLYIVRPGWLAEQAANLAHDRPGTQTAVFPNAGHALFIDEAAQFNA
                     LVDGFISRTVWP"
     gene            1797..2189
                     /locus_tag="VKQ27_13110"
     CDS             1797..2189
                     /locus_tag="VKQ27_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89914.1"
                     /translation="MALLPLFLVSLAAVGYETALIRYFAVAKWSEYGYWVISIVMVGF
                     ALSGVILALFRDACARHGEALLTALPPLLIIAAALAITLPPPTRSIRCNCRTLRPGSR
                     SSGTLQSTTSACFPSSSSPAPSSASASC"
     gene            2075..>3332
                     /locus_tag="VKQ27_13115"
     CDS             2075..>3332
                     /locus_tag="VKQ27_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89915.1"
                     /translation="MQNPTTWEPQLWNIAEYYLCLLPFFFLAGAFISLSFVLNAERIG
                     RVYGYDLTGAGAGAALVLAAMFVVHAFFLVPLLLLPLAASACFIAGRRRWAGIAAAVI
                     VLLGAEALLLLDNEAQYNDFKAIYAPLHTPDARVAATVLSPRGDYQLLDDFTERVDTD
                     VSNDAGMLGLSGPPRTYGLYRDGNRIAALPKSEPLDVGYAAASLDALPYTLIPHANVL
                     LAGASGGFRIAETLALGAGHVHVLEPEPVLLRALQDGLGPAPAWKADPRVRLSGEGPV
                     AALRGGSSYDIIDLSADFLDASEANATAFTAQAIAGYLGMLSPGGIVSIPVSIRDFPV
                     YALRMLATARAALLQAGVADPAAHAIIYRSAWSARILLSNQPWSAARITAVRKFCDDR
                     SFDVSWYPGLDPANARASIYNDLPAVS"
BASE COUNT          515 a         1144 c         1036 g          637 t
ORIGIN      
        1 ctatcagggc ggccggcggc agggcatgct gcatcagcag cgcctcgatc tcctcgggca
       61 gggcagggtt cgccagaacc atcggcgaga tgttcaccgc gacgctgcaa ctgggatgcc
      121 gggtgcgcca gtgttggcag gccgccagcg cagcctctag aatcctgcgg gtcagcggaa
      181 caatcaggcc attgtgctcc gccaggggga tgaactgatc agggtgtacc gggccgtgtg
      241 gtgggtgctt ccagcgagcc atcgcctgga atccaactac atgccgctcg accaatgtga
      301 ccttcggttg gaacgccgcg atgatgtcgc cgcgatcgag cgcctcggcc agttcggccg
      361 cggtgggcac cgcgacgggg gaactgcccc ggagccgccc gtgcggcggt agctggtcca
      421 gcaaggcgcg caaggccgac ggcccgacag gtttcgccag tgcgcccgcc acccgaagcc
      481 caagtgccac ggcatagcga gcgctggccg cgcgcacccg atcatccagg cgtgacatca
      541 gcaccaccac gggggtcgga cgcggcggct gcaatctgcc gtagcagaat gattccctcc
      601 cttccgccga gcgacagatc gagcacgatc acgtcaaatg tcgctgaggc gaaccggtgc
      661 gccgtttcat ccagcgaggt tgcggcttcc gcatcaaagc tggccgctgc catggctcgc
      721 gtaacgatca ggcactgtac cggttcgtcg tccaccacca gcaagcgccg tggcgctccc
      781 aattgagtat ccaaatgcac ccccgggtcc gtcgcgcgaa acgtcgcgca agtattagtg
      841 gcaggcatga agaacccgtg aagcgcatca acacggacat tgacacggcg cggcgcaact
      901 cggctttctg gatgcatgcg gatgtctttg cgcctgcacc tgatcgtgct cggcctgctg
      961 atgctgccgg ccagttccgc gttcgcccag gctgcggtcg aggatcagta ttttattacg
     1021 agtgacagcg tccggttgca ttacctggag gctggcccgc gcaccggcca taccatcgtc
     1081 ttcgtgcccg gctggaccat gcctgcctgg atctggcaac cgcagttgca ggcattcgcg
     1141 cggcgctacc acgtcgtggc gttcgatccg cgcggtcagg gcgagtcgga tgcgccggcg
     1201 cgcggctacg agccgaaccg ccgcggccag gatatcgccg aactcgtcgc gcatctcgag
     1261 cccgtgcccg tcgttctggt tggctggtcg ctgggtgtgc tcgattcact cgcctatctg
     1321 cacacgcatg gcgaccagaa ggttgccggc ctcgtcctgg tggacaattc ggtgggtgag
     1381 gaaccgccac cgccgccccg cccaccgcat cgtccggcac cggtactgcc gcacgcgatc
     1441 gccatgcacc gatttgtcat gtctatgttc caccatccgc aatcccaggc ctatctcgat
     1501 cggctcaccg aggcgacgct gcgcacccct gagaccgcga gccgcgcgtt gctcgcctat
     1561 ccggtgccgc gcacctactg gcgcgatgca ctattctcca ccagcaagcc ggtgctctat
     1621 atcgtgcgtc cgggctggct cgccgagcag gccgcgaacc tggcgcacga tcgtccagga
     1681 actcagacgg cggtgttccc taatgccggc cacgcattgt ttatcgacga agcggcgcag
     1741 ttcaacgcgc tggtggatgg cttcatcagc cggaccgtct ggccgtgatg ggccgcttgg
     1801 ccctgctgcc actcttcctg gtctcgctgg cggccgttgg atacgaaaca gcactgatcc
     1861 gctatttcgc cgtcgcgaaa tggtcggaat atggctactg ggtcatttcc atcgtcatgg
     1921 tgggcttcgc gctgtccggc gtgatcctgg cgctgttccg ggacgcgtgc gcgcgtcacg
     1981 gtgaggcgct gctgaccgcc ctgccgcccc tgctgatcat cgccgcggcc ttggctatca
     2041 ctttaccacc accaacccgt tcaatccgct gcaattgcag aaccctacga cctgggagcc
     2101 gcagctctgg aacattgcag agtactacct ctgcctgctt cccttcttct tcctcgccgg
     2161 cgccttcatc agcctcagct tcgtgctgaa cgccgagcgc atcggccgcg tctatggtta
     2221 tgacctgaca ggcgccggcg ccggcgccgc tctggtcctg gcggcaatgt tcgtggtgca
     2281 cgcgttcttc ctggtgccgc tcctgctgct gccgcttgct gccagcgcct gcttcattgc
     2341 cggccgtcgc cgctgggccg gcatcgccgc ggccgttatc gtgctgctcg gcgccgaggc
     2401 attgctgctg ctcgacaacg aagcgcagta caacgacttc aaggcaatct atgcgccgct
     2461 gcacacaccc gacgcgcgtg tcgctgccac ggtgctgtcg ccgcgcggcg actaccagct
     2521 gctggatgat ttcaccgagc gcgtcgatac cgatgtctcc aacgacgccg gcatgctcgg
     2581 tctgtccgga ccacctcgaa cctatggcct gtatcgcgat ggcaaccgca tcgccgcgct
     2641 gccaaagtcc gagccattgg atgtcggcta tgccgcggcg tcgctcgacg cgctgccata
     2701 cacgctgatc ccgcatgcca acgtgctgct ggctggtgca tccggcggtt tccgcatcgc
     2761 cgagacgttg gctctcggag ccgggcacgt tcatgtgctg gaaccggaac cggtgctgct
     2821 gcgagcgttg caggacggcc tgggtcccgc gccagcctgg aaggcggatc cgcgcgttcg
     2881 cctgtccgga gaaggccccg tcgccgcgct gcgcggaggg agcagctacg acatcatcga
     2941 cctgtccgcg gattttctcg acgcgtccga ggccaacgcc acagcgttta ccgcgcaggc
     3001 gattgccggt tacctcggga tgctgtcgcc cggcgggatc gtgtccattc ctgtgtcgat
     3061 acgcgatttt ccggtctatg cgctgcgtat gctggcaacc gcgcgcgccg cgctcctgca
     3121 ggccggcgtc gccgatcctg ccgctcacgc catcatctat cgttccgcct ggagcgctcg
     3181 catcctgctg tccaatcagc cctggagcgc ggcgcgcatc acggcggtgc ggaaattctg
     3241 tgacgatcgc tcgttcgatg tgtcgtggta tccaggtctc gatccagcga acgcgcgggc
     3301 cagcatctat aacgacctgc cggcagtctc tt
//
LOCUS       DATGEM010000411        12925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_426024, whole genome shotgun sequence.
ACCESSION   DATGEM010000411 DATGEM010000000
VERSION     DATGEM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426024"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..526)
                     /locus_tag="VKQ27_13120"
     CDS             complement(125..526)
                     /locus_tag="VKQ27_13120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY89916.1"
                     /translation="MAAAIDLKAEFGRLTMLRNRTPASTEAERKGAFGRVATYRDGGI
                     FTAKFAGTSAWERHPQGDEIVQVIEGATTLHLITEEGRQSLSLSAGMMTIVPRNAWHQ
                     FVAPDGVCVMTATPQPTEHVRLDVEDPRTVG"
     gene            705..1055
                     /locus_tag="VKQ27_13125"
     CDS             705..1055
                     /locus_tag="VKQ27_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY89917.1"
                     /translation="MPIRRLQPEGRLSGAVTHGNTVYLAGQVADDPSLDAEGQAADIL
                     RQIDALLAEAGTDKSRLLSCQVFLADMADVAAMNRAWDAWLDTANPPARATVEAKLVD
                     PRWKVEITGIAALP"
     gene            1106..2041
                     /locus_tag="VKQ27_13130"
     CDS             1106..2041
                     /locus_tag="VKQ27_13130"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HLY89918.1"
                     /translation="MAGIDAHWLRSTLFTVALVALVAFAIGADGPTTVSSLVTCGVGF
                     GFFYLLFPGGAHFGMTVANFLAIYACMFEFFRHANFPLASRTVALAAIAMPVLGFLLG
                     CFWHRRQVYSIIHARRIRELERLPRLTFWFIGALAVGAGSFALPRLELDGDQQGFALI
                     AAMAMITILVITWVRDVVLVIIDVAMVFEGVATRLDRLVRPMIAFLTFYALLVVVFGC
                     LYRIADITSSQPQFTLHSVPARIGFVDAIYYSVATITTLGFGDIAPASPLVRALTAME
                     VVSGVLMLLFGFSEIMRSAGADRTDRRPPGRGPDA"
     gene            2132..2635
                     /locus_tag="VKQ27_13135"
     CDS             2132..2635
                     /locus_tag="VKQ27_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLY89919.1"
                     /translation="MANAPVAVKQTATPAASTPDMWHSFRTEMDRLFDRFANWNMRPF
                     GHMFETGNGNGALTPVVDITENDTAYQMTAELPGMTEKEIELLLSGDMLTLRGEKRYE
                     KEQNDRNFHLSERSYGTFLRSFAVPDGVDRDKVSANFGNGVLTITLPKTAKAMEQQKK
                     IEVKAAA"
     gene            2941..3492
                     /locus_tag="VKQ27_13140"
     CDS             2941..3492
                     /locus_tag="VKQ27_13140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HLY89920.1"
                     /translation="MFPTPKRVFLTKGIGVNRHPLSAFELALRDADIEQQNIVYVSSI
                     LPPHCTLISRETGIEDLRPGDITFCVMARAETNELGRHVYASIGLARPTDPDMYGYIS
                     ELHGFGKTGQASGEQSEDLAATMLASTLGLDFDPDAAWNERRQIYEHSNLIIDPTSIT
                     AVAECADENQWTCAIAAAVFLFT"
     gene            complement(3565..4896)
                     /gene="purB"
                     /locus_tag="VKQ27_13145"
     CDS             complement(3565..4896)
                     /gene="purB"
                     /locus_tag="VKQ27_13145"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HLY89921.1"
                     /translation="MVPRYTRPQMEAIWAPANRYRIWFEIEALAAEGMALVGAIPESA
                     ARNIREKGGAKVAAISQADIDRIDEIERETRHDVIAFLTWLAEAIGEDSRFVHQGMTS
                     SDVLDTCLSVQMAQAGDLLLGDLDEVLAALKGRALEHKYTLTIGRSHGIHAEPTTFGL
                     KLAGHYAEFARDRARLVAARAEIAVAAISGAVGTYAHIDPRVEAYVAEKLGLAAETVS
                     TQVIPRDRHAMFFATLAVIASGIERLATEVRHLQRSEVREAEEFFHPGQKGSSAMPHK
                     RNPVLSENLTGLARVVRAAVVPALEDVALWHERDISHSSVERVIAPDATIALDFALGR
                     LAGMMQRLVVYPERMAANVESLGGVVHSGEVLLALTRAGISREDAYAIVQRNAMATWT
                     KLGTPEGRSFRDNLLDDPVVARRVSAAELEAAMDARLHLKAVDAVFARVFG"
     gene            4969..5571
                     /locus_tag="VKQ27_13150"
     CDS             4969..5571
                     /locus_tag="VKQ27_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003083.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HLY89922.1"
                     /translation="MEHLALTQDLAALAQVVLIDVTLAGDNAVVVGMAVAGLPTRRRR
                     TAIAFGIAGATAIRIALGAIALQLLEVIGLLLAGGLLLLWVCWRMFRELRGARRHAPG
                     ETPRKTLSQAMFQIILADLSMSLDNVLAVAGAASGSTWVLATGLLLSVVLMGVAANLM
                     ASMLERHRWIAWFGLLVVLYVSVSMIWHGSHEVARAVVAG"
     gene            5667..6182
                     /locus_tag="VKQ27_13155"
     CDS             5667..6182
                     /locus_tag="VKQ27_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HLY89923.1"
                     /translation="MQSNRLSAIFSHVAQWTAQQCGRASTFILACVIILVWAVTGPVF
                     NYSDTWQLIINTGTTIVTFLMVFLMQNTQNRDTVSIQLKLDELIRANENARNAMLSLE
                     DLTEEQLKRIKATFANLAEPPMQAEHKLAAASEDLEAASEKLEQAQERVLDAVETAKV
                     PTPAHDNAKTS"
     gene            6258..6974
                     /locus_tag="VKQ27_13160"
     CDS             6258..6974
                     /locus_tag="VKQ27_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY89924.1"
                     /translation="MSVKRPVLIVDDDATMLEMLSEHLDATGEFVTTTAASIEDADRA
                     LQHEDAHFDAVVLDIGLPDGDGRDYCARLRKQGHNMPIIMLTGANEEHDVVRGLDSGA
                     ADYVAKPFRASELVARLRAQLRIFERSEEAVFPVGPYVFRPAKKLLQDTGANRRIHLT
                     DKETAVLKFLYRSDKTVDRDILMHEVWGYNSAVRTHTLETHIYRLRQKIEADPSNPSL
                     LVTEGGGYRLRPEVTHRDQL"
     gene            7072..9108
                     /gene="tkt"
                     /locus_tag="VKQ27_13165"
     CDS             7072..9108
                     /gene="tkt"
                     /locus_tag="VKQ27_13165"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLY89925.1"
                     /translation="MAALDAPVTDNPVLAHQAEVLARPPTQAWQMANAIRALAIDAVE
                     AAKSGHPGMPMGMADVATALWTRFHKFDAADPRWPDRDRFVLSAGHGSMLLYALLYLT
                     GHDGMAIEQLQQFRQLHSPAAGHPEFGLHPAIETTTGPLGQGISTAVGMALAERLLAA
                     RFGRSLVDHRTWVIASDGDLMEGVSHEAAALAGHLCLSKLTVLYDDNHISIDGDTAAS
                     YSDDVLKRFTAYGWAARRIDGHDPAQIGAALSFAVRSKKPTLIACRTIIGLGAPTKAG
                     TAATHGSPLGGQEAAGAKQALGWHEPPFTVPDDLLQRWHEAGSRGAGARRAWLKRLAH
                     SSMQAEFERVMAGKLPDTWHEAVAALKQQFLETRPKVASRQSSQTTLDALVPATPELI
                     GGSADLTGSNLTFVKTMGTVTPGNFNGRYIHYGVREHGMTAAANGMALHGGLIPYTGT
                     FFVFTDYMRPAIRIGAITRAHVIHVLTHDSIGLGEDGPTHQPIEHLASLRAMPHLHVY
                     RPADAIEVAECWELAIRRNDGPSLLVLTRQGLPTMRSDIVENRTARGGYVLAEADGQR
                     AATLIATGSEVSIAMDARAALAAEGVNVAVVSLPCWELFEVQDEAYRMQVLGGAPRFG
                     IEAACGFGWERWLGSDGVFIGMTDFGASAPYEVLYKHFGITPEAIAAAVRKKLG"
     gene            9179..10192
                     /gene="gap"
                     /locus_tag="VKQ27_13170"
     CDS             9179..10192
                     /gene="gap"
                     /locus_tag="VKQ27_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HLY89926.1"
                     /translation="MPVKVAINGFGRIGRLVLRAMIESGRDDVVPVAINDLGSTDANA
                     HLFHYDSVHGRFPGQIEVSGDTITVRYGGRVWGPIKVSAERDPTKVPYQGIDVAMECT
                     GIFTSKEKASALIQAGARKVLISAPGDHVDATIVYGVNHKTLTKDMTVVSNASCTTNC
                     LAPMAKVLHDSFGIERGYMVTIHAYTGDQNTVDTLHKDLHRARAAAVSAIPTSTGAAR
                     AVGLVMPDLKGKLDGTAIRIPTPNVSLVSLDVNLKKTPTKDEVNAAMLDASKGALRGI
                     LDYNTAPLVSIDFNHSPASCTFDATQTAVVDGALTRVMGWYDNEWGFSNRMSDTAALF
                     GSL"
     gene            10317..11504
                     /locus_tag="VKQ27_13175"
     CDS             10317..11504
                     /locus_tag="VKQ27_13175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLY89927.1"
                     /translation="MSFRTLDNLDVAGKRVLLRADLNVPVRDGKITDLTRIERLTPTI
                     RELVGKGAKVIVCSHFDRPKGRRVPEMSLGPVAAALGEVLGQRVRFAEDCVGLPAEQA
                     VDRMQQGDVLVLENTRYHAGEEKNDPTFAAAMAKLADIYVNDAFSAAHRAHASTEGVA
                     HKLPSYAGRLMQAELEALHAALGNPVRPVAAVVGGSKVSTKLDLLGNLVAKVNVLVIG
                     GAMANTFLAAQGIGVGKSLQEAEMHGTANDILARARSAGCEVVLPSDAVVATELKPGV
                     ATQTVPVNAVPNDAMILDVGAATVAALTKHLATLKTLVWNGPLGAFETPPFDAATDAF
                     AKAVADATQKGALRSVAGGGDTVSALRHAGVLDKMSYVSTAGGAFLEWLEGKTLPGVA
                     ALG"
     gene            complement(11566..12486)
                     /locus_tag="VKQ27_13180"
     CDS             complement(11566..12486)
                     /locus_tag="VKQ27_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY89928.1"
                     /translation="MINLHDIRYARLGTPDLETAIKFATDIVGLQLTAREGRTAYFRS
                     DKVDVHGDTRDHTLVYFEGDPADQTIGLELIDPDDLDTVATELELVDRPVHWGTRDEC
                     DARRTRAFIATSDPSGNKIEILARPHNSGVRYFPGRDVGITNFSHVALFSTDTRRDTE
                     FWTRLANARVSDWLGDATFLRIGTVHHSVVLMPSTRPGIQHINHQVEGIDDVMRSYYW
                     LKQQGVKIVYGPGRHPISTAIMVYFEGPDGMVYEYSTGVKHILPEQEATHRPRQFSVE
                     GYNGDMWGSFADTEGLPREPVTGRRMRLVV"
     gene            complement(12606..>12925)
                     /locus_tag="VKQ27_13185"
     CDS             complement(12606..>12925)
                     /locus_tag="VKQ27_13185"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399317.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLY89929.1"
                     /translation="HWTIDACPASQFELHIRSIAGLPLPPAVRHSDAVMKNLVGPEET
                     ALWPAILGTLGLIPHLYGKAEARPGRKMGHVTRLFPRGSLPGEFGITAALGPAAPRGD
                     PAS"
BASE COUNT         2281 a         4606 c         3845 g         2193 t
ORIGIN      
        1 cgccatcagc aacaacagcg acgtggcgaa cagccggtgg cggcggagcg ttcggcgcgc
       61 ctcggcgtcc gggtctggcc cttccttggg caaaatctgc gtccgactta tcgggcttcg
      121 ccgatcaccc aaccgtcctg ggatcctcga catcaagccg cacgtgctcg gtgggctgcg
      181 gcgtcgcggt catcacgcac accccgtcag gcgccacgaa ctggtgccac gcgttgcgcg
      241 gcacgatggt catcatcccc gccgacaatg acagcgactg cctcccttcc tcggtgatca
      301 ggtgcagcgt cgtcgcgccc tcgatcacct gcacgatctc gtcgccctgc ggatgccgct
      361 cccacgcact ggtgcccgcg aacttggcgg tgaagatccc gccgtcccgg taggtcgcaa
      421 cccgcccgaa ggcgcctttc cgttccgcct cggtcgaggc cggcgtgcgg ttccgaagca
      481 tcgtcagcct gccgaactcg gctttgagat cgatcgcggc ggccatgcgc ttctcctcga
      541 caactgaatg cagttgcagc cattctaggc caccgccgca acgcctggaa ttggcgagcg
      601 atcgcttgag gccgcgacgc ccgccaattt atcggcatct gacgatgccc tggcccggta
      661 acgtccggcc gggcgttttt ctcctcatca tgagaggtac gcacatgccg atccgccgtc
      721 tgcaacctga gggccgcctg tcaggcgccg tcacccacgg caatacggtc tatctcgccg
      781 gccaggtggc cgacgatccc tcgctcgatg ccgaaggcca ggccgcggac atcctccgcc
      841 agatcgatgc cctgctggcc gaagccggca ccgacaagtc gcgccttctg tcatgccagg
      901 tgttcctggc agatatggcc gatgtcgccg ccatgaaccg cgcctgggat gcgtggctcg
      961 acactgccaa cccgccggcg cgcgccaccg tggaggcgaa gctggtcgat ccacgctgga
     1021 aggtcgagat caccggcatc gccgcgctgc cgtgacactg ccgccggttc gcgttcgcgt
     1081 tcgcgttcgc ccgcgccacc gtccagtggc cgggatcgac gcacattggc tgcgctcaac
     1141 gctgttcacc gtggcgctgg tcgcgctggt ggccttcgcc atcggtgccg atggcccgac
     1201 gacggtgtca tccctggtta cctgtggcgt cggcttcggc ttcttttatc tgctgttccc
     1261 tggcggtgcg catttcggca tgaccgttgc gaacttcctc gccatctacg cctgcatgtt
     1321 tgagttcttc cgccacgcca acttccccct ggcgtcgcgc actgttgctc tcgccgcgat
     1381 cgccatgccc gtgctcggct ttctcttggg ctgcttctgg catcgccggc aggtctactc
     1441 gatcatccac gcgcgccgta tccgggagct ggaacggctg ccccgcctca ccttctggtt
     1501 catcggagcg ctggcagtcg gtgccggttc cttcgcgctg ccgcgactcg agctggatgg
     1561 cgaccagcaa ggcttcgcgc tcatcgcggc aatggcaatg atcacgatac tcgtcatcac
     1621 ctgggtgcgc gacgtggtgc tggtgatcat cgacgtcgcc atggtgttcg aaggtgtcgc
     1681 gacccggctc gatcgcctgg tacggccaat gatcgcgttc ctgaccttct acgccctgct
     1741 cgtcgtcgtc ttcggctgcc tgtaccgcat cgccgacatc acttcgtccc agccccagtt
     1801 caccttgcac tcggtgccgg cgcgcatcgg ctttgtcgac gcgatctatt acagcgttgc
     1861 cacgatcacc acccttggct tcggcgacat tgcgccggcc tcgccgctgg tgagggcatt
     1921 gaccgcaatg gaagtggtat ccggtgtgct gatgctgctg ttcggcttca gcgagatcat
     1981 gcgcagtgcc ggcgctgatc gtaccgatcg gcgtccgccc ggtcgcgggc cggatgccta
     2041 gcgtgccact tggggtcgtt tgatctgtat caatgatccc cggagccata aggagtacac
     2101 agcccagccg gcgcaaccag ggagcaaaac aatggcaaac gcaccagttg cagtgaagca
     2161 gaccgccacg cctgctgcca gcacgccaga catgtggcac tccttccgca cagaaatgga
     2221 tcgcctgttc gatcgcttcg ccaactggaa catgcgaccg ttcggccata tgttcgaaac
     2281 cggcaatggc aatggcgcgc tgacgcccgt cgtcgacatc acggaaaacg ataccgccta
     2341 tcagatgacc gccgaacttc ccggaatgac cgagaaggag atcgagctcc tgctctccgg
     2401 tgacatgctg acgctcagag gcgagaagcg ctacgagaag gagcagaacg acaggaactt
     2461 ccatctgtcc gaacgctcct acggcacctt cctgcgcagc tttgccgtgc cggacggcgt
     2521 cgatcgcgat aaagtcagcg cgaatttcgg caatggcgtg cttaccatca ccctgccgaa
     2581 gacagcgaaa gccatggagc agcagaagaa gatcgaggtg aaggcagctg cctgaagcat
     2641 cggcaccaac gccaggtcga caacggtcag aagcgtgtcc gattgcgaat tgcgccgagc
     2701 attcgcggag ttcggaccat gttgagacat gtgaagtcaa tgcgagacgg cgcggaaacg
     2761 tcccgccgtc agcttgatca gctgcgggac ttcaggcggc agctgtcaga caggctgttg
     2821 gcatgggata cgcgcgcagc cgtcgaggct gaacaccgtt gcgagcgaat cagcgctacg
     2881 attgcccgcg gcgccaggag atgtctcggg cgtccttgag ccaaatccag aggcccgaaa
     2941 atgttcccta cgcccaagcg cgtgttcctg accaagggga tcggcgtcaa ccgccatccc
     3001 ctttccgcct tcgaactggc cctgcgcgat gctgacatcg agcagcagaa tatcgtctat
     3061 gtctcgtcga tcctgccgcc ccattgcacc ctgatctccc gcgaaactgg tatcgaggac
     3121 ctgcggcccg gcgacatcac gttctgcgtg atggcacgtg ccgaaaccaa tgaactcggc
     3181 cggcacgtct atgccagcat cggcctggcc aggccgaccg atccggacat gtacggctac
     3241 atctcggaac tgcacggctt cggcaagaca ggtcaggcca gcggcgagca gtccgaagac
     3301 ctcgcagcca ccatgctggc atccacgctc gggctggatt tcgatcccga cgccgcgtgg
     3361 aacgaacgca ggcagatcta cgagcacagc aacctgatca tcgacccgac atccatcacc
     3421 gccgtcgccg aatgcgccga cgaaaaccaa tggacctgcg ccatcgccgc cgcggtgttc
     3481 ctgttcacgt agcagcctgt cattgcgagg accccggcga aggccgggga cggagcaatc
     3541 ccgaacgacc ttgggtaccg ccgattaccc gaacacccgc gcaaacaccg catcgacagc
     3601 cttcagatgc agccgcgcat ccatcgccgc ctccagttcc gcggccgaca cgcgccgcgc
     3661 caccaccgga tcgtccagca ggttgtcgcg gaagctgcgg ccctccggcg tgcccagctt
     3721 cgtccacgtc gccatcgcgt tgcgctgcac gatcgcatat gcgtcctcgc gcgagatgcc
     3781 ggcacgggtc agcgccagca gcacctcgcc cgaatgcacc acaccgccca ggctctccac
     3841 attcgccgcc atgcgctccg gatacaccac cagccgctgc atcatccctg ccagccgccc
     3901 cagcgcaaaa tccagcgcga tcgtcgcgtc cggcgcgatc acccgctcca ccgaggaatg
     3961 cgaaatgtcg cgctcgtgcc acagcgcaac gtcctccagc gccggcacca ccgcagcgcg
     4021 caccacgcgc gccagcccgg tcaggttttc gctcagcacc gggttccgct tgtgcggcat
     4081 cgcgctggag cccttctggc ccggatggaa gaactcctcc gcctcgcgca cctcgctgcg
     4141 ctgcaggtgc cgcacctcgg tcgccagccg ctcgatgccc gacgcgatca ccgccagcgt
     4201 cgcaaagaac atcgcgtgcc ggtcgcgggg aatcacctgc gtagatacag tctccgccgc
     4261 cagcccaagc ttctcggcga catacgcctc aacgcgcgga tcgatatgcg cgtatgtacc
     4321 cacggcgccg ctgatcgccg ccaccgcgat ctccgcccgc gccgcgacca gacgcgcccg
     4381 gtctcgcgcg aactccgcgt aatgccccgc gagcttcagc ccgaacgtcg tcggctccgc
     4441 gtggatgccg tggctgcggc ctatggtcag cgtgtatttg tgctccagcg cgcgcccttt
     4501 cagtgccgcc agcacctcat cgaggtcacc cagcagcagg tcgcccgcct gcgccatttg
     4561 caccgacaga caggtgtcca gcacgtcgga gctggtcatg ccctggtgca cgaatcgact
     4621 gtcctcgcca attgcctcgg cgagccaggt caggaacgcg atcacgtcat gccgcgtctc
     4681 gcgttcgatc tcatcgatcc gatcgatatc ggcctggctg atcgccgcaa ccttggcccc
     4741 gcccttctcg cggatattcc gtgccgcgct ctccggaatg gcgccaacca gcgccatgcc
     4801 ttccgccgcc agcgcctcga tctcgaacca gatgcgatag cggttggcgg gagcccagat
     4861 cgcctccatc tggggtctgg tgtaacgggg aaccatggct gcgctgtccc gtcctggtgt
     4921 cgcgccagct agaccgctgc accaccgcct gacaagtgcc ggagccagat ggaacacctt
     4981 gcgctgaccc aagacctcgc cgcgctggcg caggtggtcc tcatcgacgt gaccctcgcc
     5041 ggcgataacg ccgtggtggt gggaatggcc gtggcgggac tgcccacccg gcggcggcgc
     5101 actgcgatcg cgtttggaat cgccggcgcc actgcaatcc gtatcgcgct cggcgccatc
     5161 gcgctgcagc tgctagaggt catcggcctg ctcctggccg gcggcctgct gctgctttgg
     5221 gtgtgctggc gcatgttccg cgagctgcgc ggcgcccggc gccatgcccc tggagagacg
     5281 ccgcgaaaga cgctcagcca ggccatgttc cagatcatcc tggccgatct gtcgatgagc
     5341 ctggacaacg tgttggccgt ggcgggagcg gcgagtggca gcacctgggt gctcgcgacc
     5401 ggcctgttgc tgtcggtggt gctgatgggt gtcgcggcga atctgatggc gagcatgctg
     5461 gaacgccatc gctggatcgc ctggtttggc ctgctggtgg tgctctatgt ctcggtatcg
     5521 atgatctggc acggctcgca cgaggtggcc cgcgcggtcg ttgcaggatg atcgcaatat
     5581 tgtgacaggc ggataaagtc cgcagccgca ccattgcccg cttcgtaagc caaccgttgc
     5641 gctccaccag tctggcgagc cgcgtcatgc aaagtaaccg cctgtccgct atcttttctc
     5701 atgtcgcgca atggaccgca cagcaatgcg gccgggcatc caccttcatc ctcgcctgcg
     5761 tcatcatcct tgtatgggca gtgaccgggc cagtattcaa ctactccgat acttggcagc
     5821 tgatcatcaa caccggcacc accatcgtca cgtttctcat ggtatttctt atgcaaaaca
     5881 cgcagaatcg cgacacggtc tcgatccagc tcaagctcga cgaattgatc cgggccaacg
     5941 aaaacgcgcg caacgctatg ctatcgttgg aagacctgac cgaagaacag ttgaagcgaa
     6001 tcaaggcaac cttcgctaac ctggcggaac caccgatgca ggcggaacac aagctggcag
     6061 cagccagcga ggatctggaa gcagccagcg aaaagctcga gcaggcgcag gagagagtgc
     6121 tggacgctgt cgaaaccgcg aaagtgccga cgccggctca cgataatgcg aagacgtcgt
     6181 gaccgatttt cgccacgaat caccgcgctt gtttgcgttg tccaaacggg ttccgacgca
     6241 atttgggggc agagaacatg agtgtaaagc gaccagtcct gatcgtggat gacgatgcga
     6301 cgatgctcga gatgctgagc gaacatctcg acgccacggg tgaattcgtc accaccacgg
     6361 cagcaagtat cgaagatgcc gatcgcgcgc tccagcacga ggacgcgcat ttcgacgccg
     6421 tcgtgctgga cataggcctc cccgacggcg acggtcggga ctactgcgcg cgcctgcgca
     6481 agcaaggcca caacatgcca atcatcatgt tgaccggcgc gaacgaggaa catgacgtag
     6541 tgcgcggcct cgactccggt gcggccgact atgtggccaa gccgttccgc gcgagcgaac
     6601 tcgtggcccg gttgcgcgcg cagctgcgca tattcgagcg cagcgaggaa gccgttttcc
     6661 ccgtcggccc gtacgttttc cggccggcca aaaaactgct gcaggacact ggcgcgaatc
     6721 gccgcatcca tctgaccgac aaggaaacgg cggtgctgaa gttcctgtac cgctcggaca
     6781 agaccgtgga tcgggatatc ctgatgcacg aggtgtgggg ctacaactct gccgtgcgga
     6841 ctcacacgct ggagacgcac atctatcgcc tgcggcaaaa gatcgaggct gatccgtcaa
     6901 atccctccct gctggtcacc gaaggcggcg gatatcgcct gcggcccgag gtgacgcata
     6961 gggatcagct gtaagtcgcg ggcaatacca caacacagca cgtcagatct cctggggttg
     7021 ccgccgccca cccatacccc tataggcgcc ggccaagctc caggagacga catggctgcc
     7081 cttgacgccc ccgtcaccga caatccggtg ctcgcccatc aagctgaggt cctggcacgg
     7141 cctcccacac aggcctggca gatggcgaat gccatccgcg cccttgcaat cgatgccgtg
     7201 gaggcagcca aatccggcca tccgggcatg ccgatgggca tggccgacgt cgccaccgcg
     7261 ctctggaccc gcttccacaa attcgatgcc gccgacccac gttggcccga ccgcgaccgg
     7321 ttcgtgctgt cggctggcca tggctccatg ctgctctacg ccctgctcta cctcaccggc
     7381 cacgacggca tggccatcga gcagctgcag cagttccgcc agctgcactc gcctgccgcc
     7441 ggccaccccg aattcggcct ccatcccgcg atcgagacca ccaccggccc gctcggccag
     7501 ggcatctcca ccgccgtcgg catggcgctg gccgagcgcc tgctcgctgc ccgcttcggt
     7561 cgctctctcg tcgaccaccg cacctgggtg atcgcctccg acggcgacct gatggaaggc
     7621 gtcagtcacg aggccgccgc gctggccggc cacctctgcc tctccaagct caccgtcctg
     7681 tacgacgaca accacatctc gatcgatggc gacaccgccg cgtcctacag cgacgatgtc
     7741 ctgaagcgct tcaccgccta tggctgggcc gcccgccgca tcgacggcca cgaccctgcc
     7801 cagatcggtg ccgcgctctc cttcgcggtc cgctcgaaaa agcccaccct gattgcctgc
     7861 cgcaccatca tcggcctcgg cgcccccacc aaggcaggca ccgccgccac ccacggctcg
     7921 ccgctcggcg gccaggaagc ggccggcgcc aagcaggcgc tcggctggca cgagccgccc
     7981 ttcaccgtcc ccgacgacct tctgcagcgc tggcacgaag ccggcagccg cggcgccggc
     8041 gcccgccgcg cctggctgaa gcgcctggcc cacagcagca tgcaggccga gttcgaacgg
     8101 gtcatggccg gcaagctgcc cgacacctgg cacgaggccg tcgccgccct gaaacagcag
     8161 ttcctcgaga cccgccccaa ggtggcctcc cgccagtcca gccagacgac gctggacgcc
     8221 ctcgtccccg cgacccccga gctgatcggc ggctcggccg acctcaccgg ctccaacctc
     8281 accttcgtca agaccatggg cacggtgacg cccggcaact tcaacggccg ctacatccat
     8341 tacggcgtac gcgagcatgg catgaccgcc gccgccaacg gcatggcgct tcacggcggc
     8401 ctgatcccct acaccggcac gttcttcgtc ttcaccgact acatgcgccc cgccatccgc
     8461 atcggtgcca tcacccgcgc gcacgtcatc cacgtgctga cccacgactc catcggcctc
     8521 ggcgaggacg gcccgacgca tcagccgatc gagcatctcg ccagcctgcg cgccatgccg
     8581 catctgcacg tctaccgccc cgccgacgcg atcgaggtgg cggaatgctg ggaactcgcg
     8641 atccgccgca acgacggccc cagcctgctg gtgctgactc gccagggcct gcccaccatg
     8701 cgcagcgaca tcgtggagaa ccgcaccgcc cgcggcggct atgtcctggc cgaagccgat
     8761 ggccagcgtg ccgcgacctt gatcgccacg ggctcggaag tctccatcgc catggacgcc
     8821 cgcgccgccc tcgctgccga gggtgtgaac gtcgccgttg tctccctccc atgctgggag
     8881 ttgtttgagg tgcaggacga agcttatcgt atgcaggtgc tggggggcgc cccgcgcttc
     8941 gggatcgagg ccgcctgcgg cttcggctgg gagcgttggc taggctctga tggggtgttc
     9001 atcggcatga cggacttcgg agcctcggca ccttatgaag ttctttacaa acactttggt
     9061 atcacccccg aggccatcgc cgccgcggtc cgcaagaagc tgggttgact caggtcaatg
     9121 ccgggcgagt gacagggcgt caggaaaggc ccggagagca acggaaggat aactgaccat
     9181 gccggtaaag gtcgccatca acgggttcgg ccgcattggc cgtctggtac tgcgagccat
     9241 gatcgagagc ggccgcgacg atgtggtccc ggtcgcgatc aacgatctcg gcagcacgga
     9301 tgcgaacgcg cacctgttcc attacgacag tgtgcatggc cgctttcccg gccagatcga
     9361 agtttcaggt gataccatca ccgtccgcta tggcggccgc gtctggggcc cgatcaaggt
     9421 gtccgccgag cgcgacccga ccaaggtgcc gtaccagggc attgacgtcg ccatggagtg
     9481 caccggcatc ttcaccagca aggagaaggc ctcggcgctg atccaggccg gcgcccgcaa
     9541 ggtgctgatc tccgccccgg gtgatcatgt ggacgccacc atcgtctacg gcgtcaacca
     9601 caagacgctg accaaggaca tgacggtcgt ctctaacgcc tcctgcacca ccaactgtct
     9661 ggcaccgatg gccaaggtac tgcacgacag cttcggcatc gagcgcggct acatggtcac
     9721 catccacgcc tataccggtg accagaacac ggtggatacg ctgcacaaag acctgcaccg
     9781 cgcccgcgcc gccgcggtct ccgccatccc cacatcgacc ggcgccgccc gcgccgttgg
     9841 cctggtgatg ccggacctga agggcaagct ggacggcacc gcgatccgca ttcccacgcc
     9901 gaacgtctcg ctggtgtcgc tcgatgtgaa cctgaagaag acgccgacca aggacgaggt
     9961 caacgcggcg atgctcgacg cctccaaggg cgcgcttcgc ggcatcctcg actacaacac
    10021 cgcgccgctg gtcagcatcg acttcaacca ctcgccggca tcttgcacgt tcgatgcgac
    10081 gcagaccgcc gtggtggatg gcgcgctgac ccgcgtgatg ggctggtacg acaacgagtg
    10141 gggcttctcc aaccgcatgt ccgacaccgc cgccctgttc ggcagccttt agtcttccgc
    10201 ttccgcccac cgtcgtggcc gggcccgacc cagccatcgg tccctccgca caccggtgca
    10261 agagaggctg atggcccggg caggctcgcc atggcgctcc accacggggg ttcgccatgt
    10321 cctttcgcac gctcgacaac ctcgacgtcg ccggcaagcg cgtgttgttg cgcgcggacc
    10381 tcaacgtgcc ggtgcgcgac ggcaagatca ccgacctgac ccgcatcgag cgcctcaccc
    10441 ccactatccg cgagctggtg ggcaaaggcg ccaaggtcat cgtctgcagc cacttcgacc
    10501 gccccaaggg caggcgtgtc cccgagatgt cgctcggccc cgtcgccgcc gcgctgggcg
    10561 aagtgctcgg acagcgtgtc cgcttcgccg aggactgcgt cggccttccc gccgaacagg
    10621 cggtggatcg catgcagcag ggcgacgtgc tggtactgga gaacacccga taccacgccg
    10681 gggaggaaaa gaacgacccg accttcgccg cagccatggc gaagctggcc gatatctacg
    10741 tgaacgacgc cttctccgcc gcgcatcgcg cccacgccag caccgagggt gtggcgcata
    10801 agctcccctc ctacgccggc cgcctgatgc aggccgagtt ggaagccttg cacgccgccc
    10861 tgggcaatcc ggtgcgtccg gtcgccgccg ttgtcggcgg ctccaaggtc tccaccaagc
    10921 tcgacctgct cggcaacctg gtcgccaagg tgaatgtcct ggtgatcggc ggtgccatgg
    10981 cgaacacctt cctcgccgcc cagggtatcg gcgtcggcaa gtcgctgcag gaagccgaga
    11041 tgcacggcac cgccaacgac atcctggcgc gcgccaggtc ggccggctgc gaggtcgtac
    11101 tgcccagcga cgccgtcgtc gccaccgaac tgaagcccgg tgtcgcgaca caaaccgtgc
    11161 ccgtgaatgc ggtgccgaac gatgcgatga tcctcgacgt cggggctgcc accgtcgccg
    11221 ctctgaccaa gcacctcgcc acgctcaaga ccctggtgtg gaacggtccg ctcggcgctt
    11281 tcgaaacccc accgttcgat gctgccaccg atgccttcgc caaggcagtt gccgacgcaa
    11341 ctcagaaggg cgcgttacgt agcgtggccg gcggcggcga cacggtgtcg gcactgcgcc
    11401 acgccggcgt gctcgacaag atgagttacg tctctacggc cggcggcgca ttcctcgaat
    11461 ggctggaagg caagacactg cccggcgtcg cagcccttgg gtagcatcag gaagaataaa
    11521 cacgaggacc agtccgcgat ctgaggtcgc cggccccatc tcgccctaca ccaccagccg
    11581 catccgccgt cccgtcaccg gctcccgtgg cagcccctcg gtatcggcga atgaccccca
    11641 catatccccg ttatatccct ccaccgagaa ctgccgaggc cggtgtgtcg cctcctgctc
    11701 cggcaggata tgcttcacgc cagtggagta ctcgtagacc atcccatccg gcccctcgaa
    11761 atacaccatg atcgccgtcg agatcggatg ccgccccggc ccatagacga tcttcacccc
    11821 ctgctgcttc agccagtagt acgagcgcat cacgtcgtcg ataccctcga cctgatggtt
    11881 gatgtgctgg atcccaggcc gcgtcgaagg catcagcacc accgaatgat gcaccgtgcc
    11941 gatacgaaga aacgtcgcat cccccagcca gtccgacacg cgcgcattcg ccagccgcgt
    12001 ccagaattcc gtgtcgcgcc gcgtatcggt gctgaacagc gcgacatggc tgaaattcgt
    12061 gatccccacg tcccgcccgg gaaagtaccg caccccgctg ttgtgtggtc gcgccaggat
    12121 ctcgatcttg ttgccgctcg gatcggatgt cgcaatgaac gcccgcgtgc gccgcgcgtc
    12181 gcattcatca cgcgtccccc agtgcaccgg ccgatccacc agctccagct ctgtcgccac
    12241 cgtatccaga tcatccggat cgatcagctc cagtccgatc gtctgatccg ccggatctcc
    12301 ctcgaaatac acaagtgtat ggtcgcgcgt atcgccgtgc acatcgacct tgtcgctgcg
    12361 gaaatacgcc gtccggcctt cgcgtgccgt caactgtagc ccgacaatgt cggtggcgaa
    12421 cttgatcgcc gtctcgaggt caggtgtgcc gagccgcgca taacggatgt cgtgcaggtt
    12481 gatcatctcg ctttaccctc cctggttgcg acaatgcgta ctcccgccag cgccaccccg
    12541 caacgccagg aaatacccgg caaccccctg gaccagtcga tcggcccgga gcgaggcaag
    12601 catcctcagg acgccggatc gccgcgcggt gccgccggcc ccagcgccgc cgtgatgccg
    12661 aactcgccgg gcagcgagcc gcgcgggaac agccgcgtga cgtgtcccat tttccgcccg
    12721 ggccgggcct ccgccttgcc gtacaggtgc gggatcagcc ccagcgtccc caggatcgcg
    12781 ggccacaacg cggtctcctc cggccccacg aggttcttca tcaccgcatc cgaatgccgc
    12841 accgccggtg gcagcggcag cccggcaatc gaccggatgt gcagttcgaa ctggctcgcc
    12901 ggacaggcat cgatcgtcca gtgac
//
LOCUS       DATGEM010000412         6639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_13587, whole genome shotgun sequence.
ACCESSION   DATGEM010000412 DATGEM010000000
VERSION     DATGEM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13587"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..1032
                     /locus_tag="VKQ27_13190"
     CDS             589..1032
                     /locus_tag="VKQ27_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLY89930.1"
                     /translation="MRVQDILRTKGSKLVSISHDASILDASRIISRERVGMLLVLDGR
                     RELVGLLSERDVTCFVGQRGASALSAPVSAAMSEAWPLAAPEDAVTRVMQLMTEERIR
                     HMPVISDGQLVGVISIGDILKSRLAEKDQETAILRDIARFSLAAA"
     gene            complement(1129..1401)
                     /locus_tag="VKQ27_13195"
     CDS             complement(1129..1401)
                     /locus_tag="VKQ27_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY89931.1"
                     /translation="MLTIDENDGTGERLDLLCGRFVLSVAHERLMRDDLPSHLVVSAA
                     EHTAATARPGTSAQLANLVGLMPLESASENLGARAMLIALSTAMFA"
     gene            1724..2260
                     /locus_tag="VKQ27_13200"
     CDS             1724..2260
                     /locus_tag="VKQ27_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY89932.1"
                     /translation="MSRIHTPALDTATGATAEVYAQIKKAAGSVPNTFATIGAHGPAA
                     LKAMLAADSVLAAGSLSKQDQETIKLLVSAVAGCDYCVAAHSLLGKMTGLPQDALKAI
                     RAGQPTGDAKRDALIHFVKLLASTSGTVSEAEFVTIRAAGYTDQQLVEISLAIAVIVF
                     TNVFNRINDTVSDFPAVD"
     gene            2379..2870
                     /locus_tag="VKQ27_13205"
     CDS             2379..2870
                     /locus_tag="VKQ27_13205"
                     /inference="COORDINATES: protein motif:HMM:NF016139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HLY89933.1"
                     /translation="MNFIADTLGRSGLLKRDLDYNLLRASMVLVFALFGYQKWWPYEA
                     DRLIPFISNGPLIFWLYPLFGHQGASWFLGASEWFFGTLLLVGFWNKKIGILGALGSV
                     ATFVGTVTILPFLPDAWDPAVGFPAMTGNMPFLLKDVVLLAVSIYLLKEDVARVLADQ
                     TSH"
     gene            2923..3048
                     /locus_tag="VKQ27_13210"
     CDS             2923..3048
                     /locus_tag="VKQ27_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89934.1"
                     /translation="MTISNETAEAQALQIILPLLGAVTAFAALIGIIAGTAIGIC"
     gene            3161..4123
                     /locus_tag="VKQ27_13215"
     CDS             3161..4123
                     /locus_tag="VKQ27_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HLY89935.1"
                     /translation="MKSVLFLVALAAVGMMGFANQRGGTCTVAAIEDIVAEHRFDKLT
                     ALFEASLWVAGGLILLATFGWLPEMPASYATGIATILGGALFGIGAFVNRACAFGSVA
                     RLGSGDWAYLATPAGFYLGALLTTHVSHPLPLIEQSILIDTPQWLAILVAALLAARIF
                     SHGWKIRRSERSVLDHVWSPHLATTVIGISFLVALVSMGPWDYTILLTDLADGAMRGF
                     ASHLALNLALLGGAIAGGWTAGRLKPVLPEPRRLIRSLVGGTIMGLGSRLIPGGNTGL
                     VLVGLPLLRPYAWLALASMCLTIYAAIRLRYRVHAGRTPSTVRH"
     gene            complement(4265..5068)
                     /locus_tag="VKQ27_13220"
     CDS             complement(4265..5068)
                     /locus_tag="VKQ27_13220"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HLY89936.1"
                     /translation="MDRHPPSPGHRAAIPFPGSPHDLCRAALDNWMAVGVSRAPEFRS
                     SIRLGQDQINDRQDTWIMTSRSSRTVASMDEAVRRLRSEIVACTHCAASLPHSPRPVA
                     QFGDAARILIIGQAPGVRVHESGVPWNDASGARLREWIGLDDITFYDPDRLALMPMGF
                     CYPGKGRSGDLPPRPECAPLWHDRILPLMPDIRLTLLVGSYAQSRYLPHAGEMSLTDR
                     VRRWATFGGNVIPLPHPSWRSTGWMARNPWFEIELIPVLQSAVHAALEP"
     gene            5344..5595
                     /locus_tag="VKQ27_13225"
     CDS             5344..5595
                     /locus_tag="VKQ27_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89937.1"
                     /translation="MVNWEQQATLIDLDGTTPLIAPLAECVRHFAVLTTAAKADARIL
                     LTQPVARAGRRTRTWILNPDEILELTAHVPDTNATARNP"
     gene            5615..6226
                     /locus_tag="VKQ27_13230"
     CDS             5615..6226
                     /locus_tag="VKQ27_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HLY89938.1"
                     /translation="MSAPQDRRLSSPSALRNRDPILAVLQDILPPSGRVLEIASGSGE
                     HILHFARHLPHLGWQPSDPSAEARASIAAWIAAEGGSNIAAPLAIDASAGSWPVERVD
                     AIIAINMVHISPWQATLGLLQSAGRLLPPEGLLFLYGPYRRTGHDFEPSNARFDADLR
                     ARNPEWGVRLLEDVSHVAEASGLRLDKIVEMPAHNLSLVFRRA"
     gene            complement(6445..>6639)
                     /locus_tag="VKQ27_13235"
     CDS             complement(6445..>6639)
                     /locus_tag="VKQ27_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89939.1"
                     /translation="DLVPRVSVESRITGGAVEIDRAHSSALPAEPTMSFHGTANQIVE
                     LATGFGVIAAHLLASIGSQA"
BASE COUNT         1154 a         2211 c         2035 g         1239 t
ORIGIN      
        1 cgactcagca ggctctgatt tcgatcgggt ccgcgagcgg acccgtcaat ggctgccgcc
       61 ccgtctcctc atcccactct cggtctcgtc atagcggggc cggcctcgcc gactggatga
      121 gcgcggagtt tgccattgcc agtggcccga caggctttgt ccatatgcgt ttcggcgtcg
      181 gcgacagccg cgaccgagat agcggctccc cggccacagc ggcgagaaag ccgacctgcg
      241 tctcattgcg ccgtgggcga ctaagtccat tgcaggagat gccgacggtc gcgagacggc
      301 agcatcagca cccccgccat atccaagatc accaatgaga cgatcgcgca tattttcgat
      361 ctttgaaaga cttatcggcg cagtgatgga ggggtaatca ataccaacca gggtaaatat
      421 gccgctcgag agaggagtgc ccgatggttc gcttcggccc ttcatgttcg atgccggacg
      481 gcaaaatggg tcgcccccac gccatgacat gcacccgacc cgatccgcgt caggcggcct
      541 cacttactcc tgccttcttc tccccgtctc actgaaagga actggaaaat gcgcgtccag
      601 gacattcttc gcacgaaagg ctcaaagctc gtttccatca gccatgatgc cagcatcctc
      661 gacgccagcc gcatcatatc ccgcgagcgg gttggcatgc ttctggtgct ggacggccgt
      721 cgcgagctgg tcggcctgct ctccgaacgc gatgtcacct gctttgttgg tcaacgcggt
      781 gcctccgccc tgagtgcgcc ggtcagtgca gcgatgtccg aagcgtggcc ccttgcggct
      841 ccggaggatg cggtcacccg cgtcatgcaa ctgatgacgg aggaacggat ccggcacatg
      901 ccggtgatat cggatgggca gcttgtcggc gtcatcagta tcggcgacat cctgaagtcg
      961 cggctcgccg aaaaggatca ggaaacggcc attctgagag acattgcccg gttctctctg
     1021 gccgcagcct gacacctaaa cggaagaggc cgggggagtg tcgaccattt tcctcggcca
     1081 ccgcgccggt cgacactgag gatgtgcgta gctgattggc cgcgatcgtc atgcgaacat
     1141 cgcggtcgac agcgcgatca gcatcgcacg agcgccgaga ttctcgctcg ccgactccag
     1201 cggcatcaac ccgacgagat tggcgagctg cgcgctcgtt cccgggcgcg cggtcgcggc
     1261 ggtatgctca gccgcgctga cgacgaggtg cgagggcaga tcgtcgcgca tcagccgttc
     1321 atgcgcgacc gagagcacga agcgcccgca caacaaatcg agccgctcgc ccgtgccgtc
     1381 attttcgtcg atcgtgagca ccggccctac gcgcgggcgt gccggtgcgg caggtgcgcc
     1441 gatccggcca gcacgatatg atagggcatc tcccccgctg cggccggatc ataagccagc
     1501 tgccacggcg cgccgaagaa gcagcgatgc tcgacccgcc cgctgaccgg caccagctcg
     1561 agcagccggc tgagtcagtc cgtgatgtcc tgcatgatca actcaatcaa tgatacgata
     1621 gggcatatta ttgagctatt gtcgaattat aaatctcgta ttttcggatc atatggcact
     1681 caacgtccga ggtggacgac ttctttgaag atggagtgtc accatgtccc gcattcacac
     1741 ccctgccctc gacaccgcga ccggcgcgac cgccgaggtc tatgcccaga tcaagaaggc
     1801 tgccggcagt gtgccgaaca ccttcgccac catcggtgcc catggtccgg ccgcgcttaa
     1861 ggcgatgctg gcagccgaca gcgtgctcgc cgccggctcg ctcagcaagc aggaccagga
     1921 gacgatcaag ctgctggtga gcgcggtcgc cggctgcgac tattgcgtcg ccgcgcacag
     1981 cctgctcggc aagatgaccg gccttccgca ggatgcgctg aaggcgatcc gcgccggcca
     2041 gccgaccggc gatgccaagc gcgacgcgct gatccatttc gtgaagctgc tcgccagcac
     2101 cagcggcacg gtcagcgagg ccgagttcgt cacgatccgg gcggccggct acactgacca
     2161 gcagctcgtc gagatcagcc tcgcgatcgc ggtcatcgtg ttcaccaacg tcttcaaccg
     2221 catcaacgac accgtctccg acttcccggc ggtcgactga ccagccggcc gggcgccgcc
     2281 ctcccccttc cccggggcgg cgcccacccc ctctcgtcgt cacccagcgg ccgcgcggcc
     2341 tgcccaccga gccctcacgc ccataacagg aggacatcat gaacttcatc gctgatacgc
     2401 tcggccgttc cggccttctc aagcgtgatc tcgactacaa cctgctccgc gcctcgatgg
     2461 tgctggtatt tgccctgttc ggctaccaga agtggtggcc ctacgaggcc gaccggctga
     2521 ttccgttcat cagcaacggc ccgctgatct tctggctcta tccgctgttc ggccatcagg
     2581 gcgccagctg gttcctgggc gcgtccgagt ggtttttcgg gaccctgctt ctggtcggtt
     2641 tctggaacaa gaagatcggc atactgggtg cgctgggttc ggtcgccacc tttgtcggta
     2701 ccgtcaccat cctccccttc ctgcccgacg cgtgggatcc cgcggtcggt ttcccggcca
     2761 tgaccggcaa catgcccttc ctgctcaagg atgtcgtgct cctcgccgtc tcgatctacc
     2821 tgctcaagga agacgtcgcg cgcgtcctcg ccgaccagac atcccactga tcctcccaaa
     2881 tccctgcggg ctgccgatcc ggcggcccgc aaaggagctt ccatgaccat ctcgaacgag
     2941 accgccgaag cgcaggctct ccagatcatc cttcccctgc tcggcgccgt taccgccttc
     3001 gccgcgctta tcggcatcat cgccggcacc gcaatcggga tatgctaacc ccatatcgtc
     3061 catcgatggc caagccggac aggaaatcca tcccccacca tcgggcgact gtccctcaac
     3121 ctttaacggc gtcccaccac cagcgcggag aggccccatc atgaagagtg ttctcttcct
     3181 tgtcgctctt gcagccgtcg gcatgatggg ctttgcgaac cagcgcggcg gcacctgcac
     3241 cgtcgctgct atcgaggaca tcgtcgcgga gcatcggttc gacaagctga cggcgctgtt
     3301 tgaggcttca ctgtgggtgg ctggcggcct gatactgctg gccacgttcg gatggctccc
     3361 cgagatgccc gccagctatg ccaccggcat cgcaacgatc ctcggcggag cgttgttcgg
     3421 catcggcgcc ttcgtcaacc gcgcctgcgc ctttggatcg gtggcgcgcc ttggttcggg
     3481 cgactgggcc tatctggcga caccggccgg tttctacctt ggcgcgctcc tcaccacgca
     3541 tgtgtcgcac ccccttccgt tgatcgagca atcgatcctg atcgacaccc cgcaatggct
     3601 ggccatcctc gtggcggcgc tgcttgctgc caggattttt tcccacggct ggaaaattcg
     3661 gcgcagcgaa cggtcggtgc tggatcatgt ctggtccccc catctcgcga cgaccgtcat
     3721 cggcatcagc ttcctcgtcg cgctggtgag catggggccc tgggattata ccattttgct
     3781 caccgatctg gccgacggcg ccatgcgcgg ctttgcatcc catcttgcgc tcaacctcgc
     3841 cctgctgggc ggcgcaatcg ccgggggttg gaccgccggc cgactgaagc ccgtcctgcc
     3901 ggagccccga cgcctgatcc gctcgctggt cggcggcacg atcatggggc ttggcagccg
     3961 gctcattccc ggcggcaaca ccggcctcgt gctcgtcggc ctgcccctgc tgcgacccta
     4021 tgcctggctt gcgctcgcca gcatgtgcct caccatctac gccgcgatcc ggcttcgcta
     4081 ccgggtgcac gccgggcgca ccccaagcac cgtccgccat tagcacgact tgagacgggg
     4141 gtccgcctcg acggagtccg atggttcata ggccgctggg ccaggcgcga cctatttata
     4201 ttcctggcga tggtcgatcc gcacctcggc cccgcacctc agccgtcgtt cgccgcggga
     4261 tcgtttatgg ttcaagagcc gcgtgcacag ccgattgcaa tacgggtatc aattcgattt
     4321 cgaaccacgg gttgcgtgcc atccagccgg tgcttcgcca cgatggatgg ggcagcggta
     4381 tgacgttccc accaaatgtg gcccaacgac gaacgcgatc ggtaagggac atctcgccgg
     4441 cgtggggcag gtagcgcgat tgggcgtagc tgccgacgag gagcgtgagc cgaatgtccg
     4501 gcatcagcgg caggatgcga tcatgccaca agggcgcaca ttcaggcctg ggcggcagat
     4561 cgccgctcct gcccttgcca ggataacaga atcccatcgg catgagggcc aagcggtctg
     4621 gatcgtagaa ggtgatatcg tcgagcccga tccattcgcg caatcgcgcg ccgctggcat
     4681 cattccacgg cactccactt tcgtgcacgc gcacgccggg tgcctgaccg atgatcagga
     4741 tgcgggccgc atcgccgaat tgcgcgaccg gacgcggcga gtggggcagc gatgccgcgc
     4801 aatgcgtaca ggcgacgatt tccgatcgca gccttctgac tgcttcgtcc atcgacgcca
     4861 cagttcggga tgatcggctg gtcatgatcc atgtgtcttg gcgatcattg atttggtcct
     4921 gcccaagccg gatcgaagac cggaattcgg gagcccggga gacgcccacg gccatccaat
     4981 tgtcgagggc tgccctgcaa aggtcgtgcg gcgatcccgg gaaagggatc gccgcacgat
     5041 ggcccgggga ggggggatgc cggtccatga agggatattc gcacgcgcca cgcaaaaggt
     5101 tacgatggat gacgcgatcg ccgccacgct gatcactttt ccgccgcggt ccacgaggag
     5161 agccgaaccg tcctgcttcc ggacgacggc gttggtgatc gcgacaattc cgcggagctc
     5221 ggcgaaacac ccctgctcga ttgcggacac atgggtcgca atgctagcag ccgggttggc
     5281 cgcggaggct gtgtctgtgg cgcgccggaa gcgacagccc gcggaaaaga ggaacagaca
     5341 ttgatggtaa attgggaaca gcaagccacc ctgatcgacc tggatggcac cactccgctc
     5401 atcgcgccgc tcgctgagtg cgtccgccat ttcgcagtgc tgacgacagc cgccaaggcg
     5461 gatgcgcgca tccttctcac ccagcctgtc gcgcgggctg gccggaggac gcgaacctgg
     5521 atcttgaatc ccgacgagat tctggaattg acggcgcatg tgcccgacac gaacgccacc
     5581 gcacggaatc cgtgaatccg gggatcgacc gacgatgagt gcaccgcagg atcggcgcct
     5641 gagttcgcct tcggcgctcc gcaatcgcga tccgatcctg gcggtgctcc aggacatcct
     5701 gccgccatcc ggacgggtgc tggagatcgc gagcggatcg ggtgagcata tcctgcattt
     5761 cgcccggcat ctgccccatc ttggctggca gccatcggat ccatcggccg aggcgcgcgc
     5821 gtcgattgcc gcatggatcg ctgcggaagg cggctcgaac atcgccgcac cgctcgccat
     5881 cgatgcgtcc gccggatcct ggccggtgga gcgtgtcgac gcgatcatcg ccatcaacat
     5941 ggtgcacatc agtccatggc aagcgacctt gggacttctg cagagcgccg gccgcctgct
     6001 gccgccggag ggcctgctgt tcctctacgg tccctatcgc cggaccggtc atgatttcga
     6061 gccgagcaat gcacgcttcg atgcggatct aagggcgcgc aatcctgaat ggggcgtccg
     6121 cttgctcgag gatgtgtcgc acgtcgcaga agcgtctggc cttcgtctcg acaagatcgt
     6181 cgaaatgccc gcccataacc tgtcgcttgt tttccggcga gcgtgacagg gtcacgctcg
     6241 ccgcgaaggt tggacgggcg cggcgacgac agttatggcc gccgagcatc tgtcgtcggt
     6301 gagggagtgg gaatattgcg gacgctgatc atattgctgc tcggaggcgt gctcggcgcc
     6361 gggctcttcc acctctatta tctccggctc gatcgggaga gccgttgttc ctgggatcat
     6421 ccgctggacc agccagcgcg gacgctatgc ttgcgagccg atggaggcga gaaggtgcgc
     6481 ggctatgaca ccaaagcccg tcgcgagctc gacaatctga ttggcagtgc cgtgaaatga
     6541 catcgtaggt tcggcgggca gcgcggagct gtgggcgcgg tctatctcga ccgccccgcc
     6601 cgtgatgcgg ctttcaacgg aaacccgagg aacgaggtc
//
LOCUS       DATGEM010000413         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_518144, whole genome shotgun sequence.
ACCESSION   DATGEM010000413 DATGEM010000000
VERSION     DATGEM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518144"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VKQ27_13240"
     CDS             <1..1911
                     /locus_tag="VKQ27_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89940.1"
                     /translation="QLFHDRASATVNGLVITDADVPAMLEICRRLDGVPLALELAAAR
                     VEAFGLNGLASLLDDRFAVLTRGPRSALTQHQTLRAMMDWSYDLLPASEQTVLGRLAV
                     FQGNFTIDGVVAVAMDTVTAAEASFEAIANLATKSLVATDISGDITYYRLLDTTRAYA
                     MEKLDASGEHRVTALRHAEYIRDFLASDDRRLDYARAIDDIRAALAWSFGSEGDIAIA
                     ASLASLSSPAWFELALPSECSHWTATALARLDPADRGTRRELVLLASLGPSWMLTKGM
                     TSEVESVLREAAELAERLDDRDYQVRTQFSLGYFYLRQPDYHSALAIGRRAEALAGRI
                     TDPLARPAADWLTGMSLYGLGELANARKHLAGVVDTDPLVSRRARIIRFGFDQRIYAL
                     GTLAMLRWMQGAPDEAIRQSNMAITEGQALGHPVSLCLGLWTGCQVALWVGDVASLER
                     SVTSLLSHTERHALENYHAYGLGFAAELSALRGDLDAGLRQMRAALDMLVRHRHHVRY
                     WAFLPNFARMQAAAGNVADGIVTVDEALEFTQRKDHTTYMPESLRIKAELSALQGDVA
                     VAEEYLTRSLALARKQGAMSWELRAAMSLARLHGHDQPARELVGSLYRRFAEGFETAD
                     MRGAKDLLEQTA"
     gene            complement(1918..2688)
                     /locus_tag="VKQ27_13245"
     CDS             complement(1918..2688)
                     /locus_tag="VKQ27_13245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HLY89941.1"
                     /translation="MSDTNALHIPNVFYGLQQYPDALAALRALVASAPNAADPNGPWF
                     IADNLITFGHTRGFLTDPRFAAAVLSEQPSPDELSIVWRTHTLCWAAESCCDVPGDIV
                     ECGSYQGYSMAVVLRYLSGMPGRHLYLYDLFDPTGGAGEGKRLPAHSPDLFEKVRTRF
                     QRYSNVTVTRGKVPQILEEVAPERIAFLHLDMNNAEAERGALETLFDRVSEGGMIVFD
                     DYGWTGYRDQKEAADKFMRMYSLHILELPTGQGLVVKR"
     gene            2799..>3218
                     /locus_tag="VKQ27_13250"
     CDS             2799..>3218
                     /locus_tag="VKQ27_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY89942.1"
                     /translation="MTLPAIRGSMTMHFGASMFFTDYSMAPGELAQALEARGFESVWA
                     PEHSHIPTSRRSPYPGGGDLPKAYYDVMDPFVTLTAAACATKKLKVATGIALVIQRDT
                     IQTAKSVASLDQISRGRFIFGIGGGWNREEIENHGTVF"
BASE COUNT          542 a          971 c         1103 g          602 t
ORIGIN      
        1 cagctgttcc atgaccgcgc cagtgccacc gtgaatgggc tggtcatcac ggatgcggat
       61 gttccggcga tgctcgagat ctgccgcagg ctcgatgggg tgccgttggc actggagctt
      121 gcggcggcac gtgtcgaggc gttcgggctc aacgggctgg cgtcgctgct tgacgacagg
      181 tttgctgtgc tgacgcgtgg gccgcgcagt gcgctgacac agcaccagac gctgcgcgcg
      241 atgatggact ggagctacga tctgctgccc gcgagcgagc agaccgttct gggccgcctg
      301 gcagtgttcc aggggaactt caccatcgat ggagttgtcg ccgttgccat ggacacggtg
      361 accgcggccg aggcgagttt cgaggccatt gccaacctgg cgacaaaatc gctggtcgcc
      421 accgatatca gcggcgacat cacatattac cggctgctcg ataccacgcg tgcctatgcc
      481 atggagaagc tggacgccag tggcgagcat cgtgtgacgg cgctgcgcca tgccgaatat
      541 atccgtgatt tcctggcatc cgatgatcgt cgcctcgatt acgcgcgggc catcgacgat
      601 atccgagcag cgttggcctg gtccttcgga tcggagggcg atatcgcgat cgcggcgtcg
      661 ctggcgtcgt tgtcgtcgcc ggcatggttc gagctggcgt tgccgtcgga atgcagccat
      721 tggacggcga cggccctcgc tcgtcttgac ccggccgatc gcggcacgcg tcgtgagttg
      781 gtgttgctgg catcgcttgg cccgtcgtgg atgctcacca aggggatgac cagcgaagtg
      841 gagtcggtat tgcgcgaggc agcggagctg gccgagcgtc tcgatgaccg agactaccag
      901 gtgcggacgc agttcagcct gggctacttc tatctccggc agccggacta ccacagtgcg
      961 ctcgcaatcg gccgccgcgc cgaggcattg gccgggcgga tcaccgatcc gttggcgcgg
     1021 ccggcggccg actggttgac cggtatgtcg ctgtatgggc tgggcgaact ggccaacgcg
     1081 cgaaaacatc tggcaggcgt cgtggacact gatcctctgg tgtcacggcg ggcgaggatc
     1141 attcgctttg gcttcgatca gcggatctat gcgcttggca cgctggcgat gttgcggtgg
     1201 atgcagggcg cgccggacga ggcgatccgg caaagcaaca tggcgatcac tgaagggcag
     1261 gcgcttggac acccggtatc gctctgcctg gggttatgga ccggatgcca ggtcgcgctg
     1321 tgggtaggtg acgtggcctc gctggagaga tccgtgacga gcctgttgag ccataccgag
     1381 cggcatgcgc tggaaaacta ccatgcgtat ggccttggtt tcgcggccga gctgtccgcg
     1441 ctgcgtggcg atctcgacgc tgggctgcgg cagatgcgcg ccgcgctgga tatgctggtt
     1501 cggcaccgcc accatgtgcg ctactgggcg ttcctgccca acttcgcgcg catgcaggcg
     1561 gcagcgggga atgtcgcgga cggcatcgtc accgtcgatg aggcgctgga gttcacgcaa
     1621 cgcaaagacc acacgacgta catgccagaa tccctgcgca tcaaggcgga gctgtcggca
     1681 ttgcagggag atgttgccgt ggccgaagag tatctcacgc gctcgctcgc cttggcgcgc
     1741 aagcaagggg ctatgtcatg ggaactgcgg gcggccatga gcctggcgcg gctgcatggc
     1801 cacgaccagc ctgcgcgcga actggttggt tcgttgtatc gccggttcgc cgagggtttc
     1861 gagactgccg atatgcgggg ggcgaaggat ctgctggagc aaaccgcgtg aacgccgtca
     1921 gcgcttcacc accagcccct ggccggttgg cagttcaagt atatgcaaac tgtacatgcg
     1981 cataaacttg tccgccgctt ccttctggtc tcggtagccg gtccagccat agtcgtcgaa
     2041 cacgatcatg ccaccctcgc tgacccggtc gaacagtgtc tccagggcgc cgcgctcggc
     2101 ttcggcgttg ttcatgtcaa ggtgcaggaa ggcaatgcgc tccggcgcga cctcttccag
     2161 aatctgcggc accttgccgc gcgtcaccgt gacgttgctg tagcgctgga agcgcgtgcg
     2221 taccttttcg aacaggtctg gtgaatgggc gggcagccgc ttgccctcac ccgcgccgcc
     2281 tgtcggatcg aacaggtcat agagatacag atgccggccg ggcatgcccg agaggtagcg
     2341 aagcacgacc gccatcgagt agccctggta cgaaccgcat tcgacgatgt cgcctggcac
     2401 atcgcagcag ctttccgcgg cccagcacaa cgtgtgcgtg cgccagacga tcgagagttc
     2461 gtctggcgac ggctgctccg acagcaccgc ggcggcgaac cgtggatcgg tgaggaagcc
     2521 gcgggtgtgg ccgaaggtga tcaggttgtc ggcgatgaac caggggccgt tggggtcggc
     2581 cgcattcggg gcactggcga ccaatgcgcg caatgcggcc agggcatcgg ggtattgctg
     2641 caggccgtag aacacgttgg gaatatgcag agcattggta tctgacattg cggcttctcc
     2701 ggtgcatcgc ccgcgagagc ttgcccgcca gcggtgaaca agttctgaac gcggggccgt
     2761 gctgtcgcgt tggccccgtc agtgcgctag gctccggcgt gacgctgcct gcaataagag
     2821 ggagtatgac catgcatttt ggcgcatcga tgttcttcac tgattattcg atggctccgg
     2881 gcgaactggc ccaggcactg gaagcgcgcg gcttcgagtc ggtgtgggcg ccggagcatt
     2941 cgcacatccc gacgtcgcgg cgctcgcctt atccgggcgg cggcgacctg ccgaaggcgt
     3001 actacgacgt gatggacccg ttcgtcacgc tgaccgcggc ggcctgcgcg acgaagaagc
     3061 tgaaggtcgc gaccggcatc gccctcgtca tccagcgcga cacgatccag accgccaaat
     3121 ccgtcgcctc gctcgaccag atctcgcgcg ggcgcttcat cttcggcatc ggcggcggct
     3181 ggaaccgcga ggagatcgag aatcacggca ccgtgttc
//
LOCUS       DATGEM010000414        10454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_788370, whole genome shotgun sequence.
ACCESSION   DATGEM010000414 DATGEM010000000
VERSION     DATGEM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10454
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788370"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VKQ27_13255"
     CDS             complement(<1..304)
                     /locus_tag="VKQ27_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HLY89943.1"
                     /translation="MLHLTKLAVGVRDIDHLRELQAERAQTSPPLRHRTRSFPRRHQE
                     VIDGGSMYWVIGGSMLVRQRIVKIIEDRRDNGEACTGLVLDPRLVPLTGRPTKPFQG"
     gene            complement(476..1018)
                     /locus_tag="VKQ27_13260"
     CDS             complement(476..1018)
                     /locus_tag="VKQ27_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89944.1"
                     /translation="MAAARSFILRRLAVVSLVAVFALPISPAHSQWHGGGGGWHGGGG
                     GWHGGGGGWHGGGGWHGGGWHGGGWGGGWHGGWGWGGWGWGPGIAFGLAVPPAYYYPP
                     PAYYPPPAYYPPPAYYPQSGYYPQSGYYPQSYSYPQSYAYGYTQGYYGTNAQQPRYAV
                     SPANDPRNCGTPDEPKACTR"
     gene            1177..1476
                     /locus_tag="VKQ27_13265"
     CDS             1177..1476
                     /locus_tag="VKQ27_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89945.1"
                     /translation="MTRILVLVFPILLAGCADQSKGSALNQCRMKYFLDDASTQQQSI
                     PGCMKAKSFETVAACNPQPDEDELDWQATAVPYSSPKCYRAIGTAPWLATFLSPM"
     gene            complement(1521..2501)
                     /locus_tag="VKQ27_13270"
     CDS             complement(1521..2501)
                     /locus_tag="VKQ27_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89946.1"
                     /translation="MPSVVILYDLDGRSRQVVTDALAGAAGATYLPDLDDAGRADALR
                     KADAVLARTTRELRQGEIALLKDIRLLQFINAGIDFVPLADLPAGVRVATNGGAYAGP
                     MAEHALAMALAAAKRLLPEHAALARGEFNQFARNRMLAGGVCGILGFGGIGIAMARLA
                     RGIGMTVHAINRRGRSEEPTDWIGTPDQIETLLAASDLLVIATPLTPATVGLIGGVQL
                     ARMKDDAILVNLARGEIIDERALYDHLVSHRGFTACIDAWWIEPVRHGKFRMDQPFMQ
                     LPNVIGSPHNSAAAIAGTGVVGLQRAIANIKRALAGEQPLYLVPLEDRMM"
     gene            complement(2501..3481)
                     /locus_tag="VKQ27_13275"
     CDS             complement(2501..3481)
                     /locus_tag="VKQ27_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLY89947.1"
                     /translation="MSGSNTFKFVMLPPQTDVTRGWGKQLAGAMPEVRVVIAEDAATA
                     EREIVDAEAAFGWLGRDLLAKAKRLRWLQAPQAAPAAGYYYPELIQHSLTVTNFREIF
                     NDHISTHIMAFVLAFARGLHVYIPQQLRREWKKPGENRGVVHLPEATALIVGVGGIGA
                     ATAQLASAFGMTVLATDARRTSAPAGVTELHKPEVLDTLLPRADFVILTVPHTPSTEG
                     FMNRARFRRMKPSAFFINIGRGMTTKLDDLVAALNAGEIAGAALDVYEQEPLPADHPL
                     WTMPNVLMTPHMAGYGPYLDDRRYDIVADNCRAFLEGKPMRNVVDKASWF"
     gene            3535..4509
                     /locus_tag="VKQ27_13280"
     CDS             3535..4509
                     /locus_tag="VKQ27_13280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HLY89948.1"
                     /translation="MKAVVCEAFGGPEVLAYRDVADPPPPAPDEIQVRIEARGVQYVD
                     VLMLAGKYQFRPEPPFIPGGEAAGTVVKVGTDVVQFRVGDRVMSRHTLGACAELGNSK
                     AALCDLVPDGMGIEAAGVFRGAYATAYHALLQRGRLKEGEWVLVHGAAGGIGIAAIQV
                     ARLFGAKVIATASTDEKRNACLEEGADHAIGYHGGFLDAVKDLTGGRGVDIVYDPIGA
                     EVTEESLRCLAWCGRLLILGFLGGGPTNIRSNYLLIKGIDAIGVRIGGLNEADPALAI
                     ANMKALTELAGQGKLKPRISHRFPLAQAAEAMQAVIDRKVIGKAVLVG"
     gene            4691..5101
                     /locus_tag="VKQ27_13285"
     CDS             4691..5101
                     /locus_tag="VKQ27_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HLY89949.1"
                     /translation="MPEPGRALPQPTPETQHFWDGAQAGELRLQRCDDCSHVYFPPRP
                     FCPKCGTRKVSVFKASGKAILDSYVIHHRKVPGFEPPYAIAVVKLVEGPRMMTNIVEC
                     PQTPEALQLDMPLEVVFAKQTDTITLPFFRPAKG"
     gene            5106..6248
                     /locus_tag="VKQ27_13290"
     CDS             5106..6248
                     /locus_tag="VKQ27_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HLY89950.1"
                     /translation="MPHNTVAVVGAAETTKLGVVPDMSQIQLHADAALNAMADCGLKP
                     KDIDGVATAGETPVQIAHYLGITPTWVDGTSVGGCSFMIHVRHAAAAIEAGLAKTILV
                     THGESGRSRVGNPPRSVPPSSLNGQFEQPFGPMGPPSLFPIPVLRYMKTYGVTHEQIA
                     MVSVVQREWAAKNPRATFKDPITVEDVLGSRMIAYPFRLLQCCLVTDGGGALILTSAD
                     RAKDFPTKPVYILGTGESVETPMVSQMESFTSSRAFSVAGPTAFREAGITHKDVDHLM
                     IYDAFAHLPLFGLEDLGFVRRGEAGRFIAERNTAPGSKLPLNTNGGGLSYMHSGMYGM
                     YALQESVRQMRGIAPAQIPGAKISVCHGVGGMFAASGTIIFSNQKP"
     gene            6245..7432
                     /locus_tag="VKQ27_13295"
     CDS             6245..7432
                     /locus_tag="VKQ27_13295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLY89951.1"
                     /translation="MSLFSDLKVIDCASWIAGPAAATILSDFGADVIKIEPPGVGDPW
                     RSRSASGTPNDYYWQLTSRNKRSLALDLKHEDGLAVLYRLVAGADVFVTNFPLPVRDR
                     LKLAPEHLLPLNPRLIYASFTAYGEAGDEAAKTGFDSTAYWARTGLMDAVRADVDTPP
                     ARSAPGMGDHPSATGLYAAIVTALYRREKTGLGGVVRSSLLQNGLWANGCFVQTRLFG
                     EPVPHRPPREDAPNALANHYRCRDGRWFIMALFNEERQLRPLLNAIGREDLADDPRFA
                     TNAARKQNARALVDELDAVFGSRDMAEWRAILDRVGITFGVVGKTDDALDDPQMHAIG
                     AIVPFADGKALTVSSPFHVDGVDKVAPRYAPKVGQHSEEVLRGAGYSADEIGRLRTLG
                     VLA"
     gene            complement(7506..8558)
                     /locus_tag="VKQ27_13300"
     CDS             complement(7506..8558)
                     /locus_tag="VKQ27_13300"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89952.1"
                     /translation="MAQANVYASVAGTVGRSDKDPGKVGVFRRAAETGEWEHVIADHN
                     SNMVFVHPTNSGLVFAGTHDGVYRSIDGGKTFRRANFPDKGMEIWSFLVDAGDPKLMY
                     AGGSPTAVYRSEDSGESWKRLPDPHMPSRCVMPFQCRVMRMAQHPKKPGTIFAALEVG
                     GVMRTTDGGETWKDCSDSLVQLAKQPHLKSKIASDSFEEGMLDGHAIAINPADPDAVI
                     VAVRMGLFRTADDGNSWQDMEVGRFSPTTYGRDIQASRAEPGTLYSALSVAAASKDGA
                     VYRSKDKGQTWQRFDKVQVHGTIMSVAPHPSDPKQVYLGARYDGEVFGTQDGGESWQS
                     MNLPQGVKDIYALACG"
     gene            8669..9718
                     /locus_tag="VKQ27_13305"
     CDS             8669..9718
                     /locus_tag="VKQ27_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HLY89953.1"
                     /translation="MPTVSAERLMDIANALLQAAGASAEEAATIARYNIGANLVGHDS
                     HGIILIPTYIDRIKVGHIVPGAPWNITQETATSTVIDGNWGFGYVVTDRAMRYTIDKA
                     KKQQVAAATVYRQSHIGRLASYPLMAAREGMIAMITADSGRSAKAVAPFGGAKARLGT
                     NPICFAVPSNLDGPLFFDMATSAAAAGKINVATARGEQVPAGWLIDAEGKPSTDPRVL
                     RQGGALLPVGGSEGYKGYGLATIVEILSGLLTGLGFGVEPTGRHNDGCFIAVFNVAAF
                     RDLDTFKREVTEFAQYLKATPPAQGFTEVLYPGEIEFKREQDRRKNGIPVEDATWKKL
                     QELAQGYGLTEKLGL"
     gene            9724..>10454
                     /locus_tag="VKQ27_13310"
     CDS             9724..>10454
                     /locus_tag="VKQ27_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY89954.1"
                     /translation="MFIGQSLQRLEDARFLTGRGRYIEDIDEPGQTWMQVVRSPHAHA
                     VIRSIDIAAARAVPGVVGVYTAVDLAGLGALPCTVPVASVAPMIVPPRFALASERVRH
                     VGDPVAFVVAETRGAARDAAELVVVDWDTLPSVVDGPAALLPGTVQLWEQAPGNLSYR
                     FQKGDQAAVQSAMASAAHVVELELVNNRLVICSLENRGAIARYDGDGFHLLFSGAGVH
                     ALQTQLADSVFHVPANQMQIACPDVG"
BASE COUNT         1955 a         3322 c         3320 g         1857 t
ORIGIN      
        1 atccctggaa cggcttggtc ggtcgcccgg tcagcggaac cagcctcggg tccagcacca
       61 gcccggtgca cgcctcgcca ttgtcgcggc gatcctcgat gatcttcacg atacgctgtc
      121 gcaccaacat gctgccgccg atcacccagt acatcgagcc gccgtcgatg acctcctgat
      181 gccggcgcgg gaacgagcgc gtgcggtgac ggagcggcgg actggtttgc gcgcgctccg
      241 cctgaagttc gcgcagatgg tcgatatcgc gcacgccaac agcgagtttc gtgagatgca
      301 gcatggtccg gcgactcgga tgtcagtgcg atgatggcag ggacggccat gcatttgaac
      361 tgcacgccgc tgagccgcga tgtgcgtgcc agagagccta cgacattaca attgcgggcc
      421 ggcagacgag ccaacgccgc gcatcacctc ccggtcgcct ccgttccagg cacgctcaac
      481 gggtgcaagc ctttggctcg tcaggtgtgc cgcaattgcg agggtcgttc gcaggcgaaa
      541 cagcatagcg cggttgctgg gcattggtcc cgtagtagcc ctgcgtgtac ccgtaggcgt
      601 aggactgcgg atacgagtac gactgcgggt aataccccga ctgcgggtaa taccccgatt
      661 gcgggtaata ggccggcgga ggataatacg ccggcggcgg gtagtaggcc ggcggcggat
      721 aataataggc cggtggtacg gcaaggccga aggcgatgcc cggtccccag ccccagccgc
      781 cccagcccca accaccgtgc cagccgccgc cccaaccacc tccatgccag ccgccgccgt
      841 gccagccacc accaccgtgc cagcccccgc caccaccgtg ccagcccccg ccaccaccat
      901 gccagccgcc accgccgcca tgccattggc tgtgggccgg agaaatcggg agcgcaaaca
      961 ccgcgaccag agagacgact gccaatctgc ggagaatgaa ggaacgagct gctgccatcg
     1021 ggatggacct tggctgaggg tttttccgaa cctggcggag ccagcgcatt gcttcgtcaa
     1081 agcccagaac gggtcatatg gcatatgggt cgactggctg ccggttgaca gaggccgacc
     1141 cgtgcagatc atcgctaccc caccagaggt aatttcatga cccgcatatt ggttctcgta
     1201 ttcccgatcc tgcttgccgg ctgtgccgac caatcgaagg gcagcgcact caaccagtgc
     1261 cgcatgaagt actttctcga tgatgcgagc acgcaacagc agtcgatacc cggttgcatg
     1321 aaggcgaagt cgttcgaaac ggtggctgcc tgcaaccctc agcccgacga ggacgaactg
     1381 gactggcaag cgacggcagt cccatacagc agtccgaaat gctaccgggc gatcggcacc
     1441 gcgccctggc tggctacgtt tctgtcaccg atgtaacaat tgctgtggcc gagccggccc
     1501 cgcccggccg agaaccagca ttacatcatg cgatcttcca gcggcaccag gtaaagcggc
     1561 tgctcacccg ccagcgctcg cttgatgttc gcaatcgcac gttgcaggcc cacaacaccg
     1621 gttcccgcga ttgcagcagc tgagttatgc ggcgagccaa tcacgttggg caattgcatg
     1681 aatggctggt ccatacggaa tttgccgtgg cgcacgggct cgatccacca ggcgtcgatg
     1741 caggcggtga agcctcgatg cgagaccagg tgatcgtaga gcgcacgttc gtcgatgatc
     1801 tcgccgcgcg ccaggttcac caggatggcg tcgtccttca tccgcgcaag ctggacgcca
     1861 ccgatcagac cgactgttgc cggcgtgagt ggtgtagcga tcaccagcag gtcggaggct
     1921 gcgagcaatg tttcgatctg gtccggcgtg ccgatccaat cggtgggttc ctcgctgcgg
     1981 ccgcggcgat tgatcgcgtg cacggtcatg ccgatgccac gtgcaagccg ggccattgcg
     2041 atgccgatgc ccccgaagcc aagaatgccg catacgccgc cggccagcat gcggttacgc
     2101 gcgaactggt tgaattcgcc gcgcgcgagc gccgcatgtt ccggcagcag gcgcttcgcc
     2161 gccgccagcg ccatcgccag cgcgtgctcc gccatcggtc cggcataagc tccgccattg
     2221 gtggccaccc gcactccggc aggcagatcg gccagaggca cgaaatcgat gccggcgttg
     2281 atgaactgca gcaggcggat gtccttcaac agcgcgattt caccctgccg caactcgcgc
     2341 gtggtgcgcg caaggaccgc atccgccttg cgcagcgcat cggcgcgtcc cgcatcgtcg
     2401 aggtccggca ggtaggtcgc accggccgcc cccgccagcg catcggtcac gacctgccgg
     2461 ctgcggccgt ccaggtcgta gaggatcacg acgctcggca tcagaaccac gacgccttgt
     2521 cgacgacatt ccgcatcggc ttaccctcca aaaacgcgcg gcaattatcc gcgacaatat
     2581 cgtatctacg atcgtccaga tacggtccat aacccgccat gtgcggggtc atcagcacgt
     2641 ttggcatggt ccacagtgga tgatcggctg gcagcggttc ctgctcatag acatcgagcg
     2701 ccgcaccggc gatctcaccc gcgttcagcg ccgctaccag atcatccagc ttcgtggtca
     2761 taccgcgccc aatgttgatg aaaaaggcgc tgggcttcat acgccggaac cgcgcgcggt
     2821 tcatgaaccc ctccgtcgat ggcgtatgcg gcacggtcag gatgacgaag tccgcccgag
     2881 ggagcagtgt atccagcacc tccggcttat gcagctccgt cacaccggca ggagcggacg
     2941 tgcgccgcgc atccgttgcc aacacggtca tgccgaatgc cgacgcaagc tgggcagtcg
     3001 ccgcgccaat gccgccaaca ccgacaatca gtgccgtggc ttccggcagg tggacaacgc
     3061 cgcggttctc gccgggcttc ttccattccc ggcgaagctg ctgcggaata tacacatgca
     3121 gcccgcgggc gaacgccaga acgaaggcca tgatgtgggt gctgatgtgg tcgttgaaaa
     3181 tctcgcggaa gttcgtgacg gtgagcgagt gctgaatcag ttccggatag tagtatccag
     3241 cggccggcgc ggcctgaggt gcctgcagcc agcgcagccg cttcgccttc gccagcaggt
     3301 cgcgcccaag ccagccaaat gccgcctcgg catcgactat ctcgcgctcg gcagtcgcag
     3361 cgtcttcagc aatgaccacc cgtacttccg gcatcgcgcc ggcgagctgc ttgccccagc
     3421 cgcgtgtgac atcggtctgc ggcggcagca tgacgaactt gaatgtgttg gaaccggaca
     3481 tggcgggccc tcccttgctg aggctagtgt ttcatggagg cggaggatga agcaatgaaa
     3541 gcggttgtgt gcgaggcgtt cggcggcccc gaggtattgg cgtatcgcga tgtagccgac
     3601 ccgcccccgc ccgctccgga tgagatccag gtgcgtatcg aggcgcgcgg cgtgcagtat
     3661 gtcgacgtgc tgatgctggc gggcaaatac cagttccggc cggagccgcc gttcattcca
     3721 ggcggcgaag cggccggcac ggtcgtcaag gtaggaacgg acgtggtcca gttccgcgtc
     3781 ggcgatcgtg tcatgagccg gcacacgctg ggagcgtgcg cggagctagg taattccaag
     3841 gctgcgctgt gcgatctggt gcccgacggg atgggtatcg aggcggcagg cgtgttccgg
     3901 ggagcatatg cgacggcata tcatgccctg ttgcagcgtg gacgcctgaa ggaaggtgaa
     3961 tgggtgctgg tgcacggcgc tgccggcggc atcggcatcg cggcgatcca ggtcgccagg
     4021 ctgttcggtg cgaaggtgat cgcaacggca agtacggatg aaaagcgcaa cgcctgcctg
     4081 gaggaaggag cggaccacgc aattggctac cacggcggtt tcctggacgc ggtgaaggac
     4141 ctgaccggcg gccgaggcgt cgatatcgtc tacgatccga tcggggcgga agtgaccgaa
     4201 gagtcgctcc gctgccttgc ctggtgcggc cgcctgctga tcctggggtt cctcggtggc
     4261 ggaccaacga acatccgcag caattatctg ctgatcaaag gcatcgatgc cattggtgtg
     4321 cgtatcggcg ggctgaacga agccgatccg gcactggcga tcgccaacat gaaggcgctg
     4381 acggaactgg ctggccaggg caagttgaag ccgcgcatct cgcatcgctt tccgctggcg
     4441 caggccgccg aggcgatgca ggctgttatc gaccgaaagg tgatcggcaa agcggtgctg
     4501 gttggatagt tcataggcat aaacacggat tacgcagatt acggcacaga tagcacggag
     4561 aaagataaac ggcgcaaagc gcccaaagaa tcgcctccgc gtaatccgtg caatctgcgt
     4621 ttattcttcc cccattgcgg cctcaatccc tgcccgttag ccttcctcag accttcggag
     4681 gaaacgcacg atgccagagc caggccgcgc cttgccgcaa cctacgcccg agacgcaaca
     4741 tttctgggac ggcgcgcagg ctggcgaact tcgcctccag cgctgcgacg attgcagcca
     4801 cgtctacttc ccgccacgcc cgttctgccc gaaatgcggc acgcggaagg tcagcgtgtt
     4861 caaggcaagc ggcaaggcaa tcctcgacag ttacgtgatc catcaccgca aggtgcccgg
     4921 tttcgagccg ccctatgcga ttgcagtggt caagcttgtc gaaggaccac gcatgatgac
     4981 gaacatcgtc gagtgtccgc aaacacccga ggccttgcag ctcgacatgc cgctggaagt
     5041 ggtgttcgcg aagcagaccg acaccatcac gcttcccttc ttccgtccgg cgaaaggctg
     5101 atccgatgcc acacaacaca gttgctgtcg tcggcgccgc ggaaaccacg aagctcggcg
     5161 tcgtccccga tatgtcgcag atccagttgc acgcagatgc ggcgctgaat gcgatggccg
     5221 attgcggatt gaagcccaag gatatcgacg gcgtcgccac cgcaggcgag acgccagtac
     5281 agatcgcgca ctatctgggc attaccccga cgtgggtgga cgggacatcg gtcggcggct
     5341 gttcgttcat gatccatgtg cggcacgcag ccgcggcgat cgaggccggg ctggcgaaga
     5401 ccatcctggt aacgcatggc gaaagcgggc ggtcgcgcgt gggcaaccca ccgcgctcag
     5461 tgccgccatc gagcctgaac gggcagttcg agcagccctt cgggccaatg ggaccgccga
     5521 gcctgtttcc tattccggtg ctgcgctaca tgaagaccta cggcgtgacg catgaacaga
     5581 tcgccatggt gtctgtcgtg cagcgtgaat gggcagcgaa aaacccgcgc gcgacattca
     5641 aggacccgat caccgtggaa gacgtgttgg gttcacgcat gatcgcgtat ccattccgtc
     5701 tgctgcaatg ctgcctggtg acggatggcg gcggagctct gatcctgacc tccgccgaca
     5761 gggccaagga ttttccgacc aagccggttt acatcctggg caccggcgaa agcgttgaaa
     5821 cgccgatggt gagccagatg gaaagcttta ccagctcgcg cgcgttcagc gttgcgggac
     5881 cgacggcgtt ccgcgaggca ggcatcaccc acaaggacgt cgaccacctg atgatctacg
     5941 atgcgttcgc gcatctgccg ctcttcggcc tggaggacct cggcttcgtg cgacgtggcg
     6001 aggctgggcg cttcatcgcg gaacgcaaca ccgcgcccgg cagcaagctg ccgctgaaca
     6061 ccaacggcgg cggattgtcg tatatgcact ctggtatgta cggaatgtat gcactgcagg
     6121 aaagcgtccg gcagatgcgc ggcatcgcgc cggcgcagat tccaggggcg aagatctcgg
     6181 tctgccatgg cgtaggcggc atgttcgccg cgtccggcac catcatcttc agcaatcaaa
     6241 aaccatgagc ctgttctctg acctgaaggt catcgactgc gcgagctgga ttgccggccc
     6301 ggcagccgcg acgatcctgt cggatttcgg tgccgacgtg atcaagatcg aaccgcctgg
     6361 cgttggcgat ccctggcgct cccgcagcgc cagcgggacg ccgaacgact attattggca
     6421 gctcacatcg cgcaacaagc gaagcctggc gttggacctg aagcacgaag atggcctggc
     6481 agtactgtat cggctggtgg caggcgcaga cgtgttcgtc accaacttcc cgctaccggt
     6541 gcgcgatcgg ctgaagctgg cgccagagca tctgctgccg ctgaaccctc ggctgatcta
     6601 cgcctcgttc accgcatacg gcgaggcggg agatgaggcg gcgaagaccg gcttcgattc
     6661 caccgcctac tgggcgcgca ccgggctgat ggatgccgtg cgggcggacg tggatacgcc
     6721 accggctcgg tcagcgcccg gcatgggcga tcaccctagc gccactggtc tgtacgccgc
     6781 catcgtcacc gcactgtatc ggcgcgaaaa gaccgggctt ggcggcgtag tgcggtcatc
     6841 gctgctgcag aacggactgt gggccaatgg gtgctttgtg cagacgcgtc tgttcggcga
     6901 accagtgccg catcgcccgc cgcgcgagga tgcgccaaac gcgctggcca atcattatcg
     6961 ctgtcgcgat ggccgctggt tcatcatggc gctgttcaac gaagaacggc agttgcggcc
     7021 cttgctgaac gcgatcggcc gggaggacct ggcagacgac ccacgtttcg cgaccaacgc
     7081 ggcacgcaag cagaacgcac gggcattggt tgatgagctc gatgccgtgt tcgggtcgcg
     7141 cgacatggcg gaatggcgcg cgatcctgga tcgcgtcggc atcaccttcg gcgtggtcgg
     7201 caagaccgac gatgcgttgg atgacccgca gatgcacgcg attggtgcga tcgtgccgtt
     7261 cgccgacggc aaggcgctta ccgtgtcgtc accgttccac gtcgacggcg tggacaaagt
     7321 cgccccccgc tacgcaccca aggtgggcca gcacagcgaa gaagtgctgc gcggcgccgg
     7381 ctactcagcc gacgaaatag gccgtctgcg caccctggga gtgctcgcct gacctgctaa
     7441 cctgtcattg cgaggagcac cgcgacgaag caatctcgaa cgaaattgct tcgtcgcaat
     7501 gacggttacc cgcacgccag agcgtaaatg tccttcacgc cctgcggcag gttcatcgat
     7561 tgccagctct cccccccgtc ctgcgtgccg aacacctcgc catcgtagcg cgcgccgaga
     7621 tagacctgct tcggatcgct tggatgcggt gccaccgaca tgatggtgcc gtgcacctgc
     7681 accttgtcga accgctgcca ggtctggccc ttgtctttgc tgcgatacac tgcgccgtcc
     7741 ttgctcgccg cggcaacgct cagcgccgaa tagagcgtcc caggttcagc gcgcgatgcc
     7801 tggatgtcgc ggccataagt ggtgggcgag aatcgaccca cttccatgtc ctgccagctg
     7861 ttgccgtcat ccgccgtgcg gaacagcccc attctcaccg ccacgatcac cgcgtcggga
     7921 tcagccggat tgatagcgat ggcatgccca tccagcatgc cttcctcgaa actatcgctg
     7981 gcgatcttgc tcttcagatg cggctgcttc gccagctgaa cgagcgagtc actgcagtcc
     8041 ttccaggttt ctccaccatc cgtggtgcgc attacgcccc ccacctccag cgcggcgaag
     8101 attgtgcccg gctttttcgg atgctgcgcc atgcgcatca cacggcattg gaacggcatc
     8161 acgcagcgtg acggcatgtg cggatcaggg aggcgcttcc agctctcacc actgtcctcg
     8221 ctgcgataca ccgccgtggg cgaaccgccg gcgtacatca gcttcgggtc gccggcatca
     8281 accaggaacg accagatctc catgccctta tcggggaaat ttgcgcgccg gaacgtcttg
     8341 ccgccgtcga tgctgcgata gacgccgtca tgcgtgccgg cgaacacgag accggaattg
     8401 gtgggatgga cgaacaccat gttgctgttg tgatcggcga tcacatgctc ccattcgccg
     8461 gtttctgccg cacggcggaa cacgccgacc ttgccgggat ccttgtcgct gcggccgacc
     8521 gttccggcca cgctggcata gacatttgct tgtgccatgg tccgctcctc ctgcatcttt
     8581 cggcgagcct acgccattgc tgccggggtt gacagtgggg ggccttcgtc gccttgctgc
     8641 cccgcaaagt tgtggaggga gaaccgcaat gccgacagta tccgccgaac gcctgatgga
     8701 catcgccaac gcgctgctgc aagccgccgg cgcctccgcc gaggaagccg cgaccatcgc
     8761 ccgctataac atcggcgcca acctggtcgg tcacgattca cacggcatta tcctcatccc
     8821 aacctatatc gatcgcatca aagtgggaca catcgtcccg ggtgcgccat ggaacatcac
     8881 ccaggagacg gcgaccagta cggtaatcga tggcaactgg gggtttggct acgtcgtcac
     8941 cgatcgggca atgcgttaca cgatcgacaa agcgaagaag cagcaggtcg ccgcagcgac
     9001 cgtgtatcga caaagccata ttggacggct ggcgtcctat ccgctgatgg ccgcgcgcga
     9061 aggcatgatc gcgatgatca ccgccgattc gggacgcagc gcgaaagccg tggcgccgtt
     9121 cggcggggcc aaggcacggc tgggcacgaa tccgatctgt tttgccgtac cctccaacct
     9181 ggacggcccg ttgttcttcg acatggccac atcggctgcg gccgcgggca agatcaacgt
     9241 cgcgaccgcg cgtggcgagc aggtgccggc aggctggctg atcgacgctg agggcaagcc
     9301 ctccaccgat ccacgcgtgc tccgtcaggg cggtgcgctg ctgccggtgg gcggatccga
     9361 gggctacaag ggatacgggc ttgcgacaat tgtcgagatt ctgtccgggc tcttgactgg
     9421 cctgggattt ggcgtggagc caacgggacg gcataacgac ggctgcttca tcgccgtgtt
     9481 caacgttgcc gccttccgcg atctggacac cttcaagcgc gaggtgaccg aattcgcgca
     9541 atacctgaag gccacgccgc cagcacaggg cttcaccgaa gtgctgtatc ccggcgaaat
     9601 tgagttcaag cgtgagcagg atcggcgcaa gaacggcatc ccggtcgagg acgccacctg
     9661 gaagaagctg caagagctgg ctcagggcta tgggctgacg gaaaagctcg ggctctgatc
     9721 agcgtgttca tcgggcaatc gctccagcga cttgaggatg cgcgcttcct caccgggcgc
     9781 ggccggtaca tcgaggacat cgacgaacct ggccagacct ggatgcaggt ggtgcgctcg
     9841 ccacacgcgc atgccgtgat ccgcagcatc gacatagcgg cggcacgagc ggtgccgggt
     9901 gtggtcggtg tctatacggc agtggacctt gccggattgg gcgcgttgcc ctgtacggtg
     9961 ccggtcgcga gcgtggcccc gatgatcgta ccgcctcggt tcgcactcgc ctctgagcgg
    10021 gtgcggcatg tcggcgaccc ggtggcattc gtggtggcgg agacgcgcgg agctgcgcgc
    10081 gatgcggcgg aacttgtcgt tgtcgactgg gatactctgc catcggtcgt cgacggaccg
    10141 gcggcattgc tgccaggaac ggtgcagctt tgggaacagg cgccgggcaa tctctcgtat
    10201 cggttccaga agggcgatca ggcagcggtg cagtcggcga tggcatcggc tgcgcatgtc
    10261 gtcgagttgg agttggtcaa caaccggctg gtgatctgtt cgctggagaa ccgcggtgcg
    10321 atagctcggt atgatggcga tggctttcac ctgcttttct ccggcgccgg cgtacatgcc
    10381 ttgcagacac agcttgcaga cagcgtgttc cacgtgccag caaaccagat gcagatcgcg
    10441 tgccccgatg tcgg
//
LOCUS       DATGEM010000415         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1196226, whole genome shotgun sequence.
ACCESSION   DATGEM010000415 DATGEM010000000
VERSION     DATGEM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196226"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..>3242
                     /locus_tag="VKQ27_13315"
     CDS             240..>3242
                     /locus_tag="VKQ27_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY89955.1"
                     /translation="MQMNVADFASLDNPADSQDSVTQRIRQEALPQSGPLQSAIFNSA
                     NFSSIATDANGVIQIFNVGAERMLGYTAAEVMGKITPADISDPQELIARAKELSAELE
                     TPIAPGFEALVFKASRAIEDIYELTYIRKDGSRFPAVVSVTALRDAQNTIIGYLLIGT
                     DNTARKRAEEALLKAGALQSAIFNSANFSSIATDANGVIQIFNVGAERMLGYTAAEVM
                     NKITPADISDPQEMIARAKALSAELETPIAPGFEALVFKASRGIEDIYELTYIRKDGS
                     RFPAVVSVTALRDAQSTIIGYLLIGTDNTARQQVEEERKKLDQRLRDQHFYTRSLIES
                     NIDALMTTDPRGIVTDVNKQMEALTGRTRDELIGAPFKNYFTDPNLAEAAICRVLAEG
                     KVTNYELTARSLEGKLTVVSYNATTFHDRDRVLQGVFAAARDITERKQYEESLREATH
                     RAEQANRAKSDFLANMSHEIRTPMNAVIGLSYLLGQTSLDEQQLAFLAKVKLASKSLL
                     VVLNNVLDLSKIEAGELIVERAPFSLRSLLKELTDVIAVQADAEGIGFEIDLPDEVPS
                     ALEGDATRLSQILTNLLSNAIKFTERGGVMLQVRLLPATSERVTLGFVVRDTGIGIAP
                     EMQARLFAPFAQADASITRRFGGTGLGLSIVKRLVGLMGGRMELHSTPGVGSEFKIEL
                     DFWLASPEVLARLDASPNEPGNNSLAGVRVLVVDDSDVNLDVTKRILESKGAQVWLAR
                     NGQQAFELIQAAPHAVDVVLMDVQMPILDGHGATRRIRLELGLTKLPIIALTAGALSS
                     ERLRATVAGMDDYLIKPFDPSALVWSILRHVKPGGSPPSRQTDGASQTPAPGAQPWPE
                     IEGIDSIEARVHVTGDLRMFQAMLERLLDEFSDVTIPAPMVDPSALAVQAGRMHKLTG
                     SAGTLGATAIQQLAGEAEAAYTAGEVDRAANLAARLAGLLRGLRRSAAAALAAYPAET
                     KEEEPFAGAVIEPREVFDLIDLLRHQNLA"
BASE COUNT          643 a         1029 c          997 g          573 t
ORIGIN      
        1 cgatgcacgg ccaactgcga gtcgacagcg agccggcgct cggaagctgt ttcaccatca
       61 ctctgcccgg cgtgcgcgaa atcgtggcct cctttgaacg tcatggtgtc gaacgccacg
      121 cccagggttt gcatgagcgc cacgaatagc gacatcgtgc cggcccgcgc cgatccggat
      181 gcgatccgtt ccgccgcatt cctgccggcc agggccgctt gcggtagcgg agatcggtca
      241 tgcagatgaa cgttgccgat ttcgcaagtc tcgacaatcc ggcagactcg caggactccg
      301 tcacccagcg cattcggcag gaggccttgc cgcaatccgg acccttgcaa agcgccatat
      361 tcaacagcgc caatttctcg agcatcgcca ccgatgcgaa cggcgtcatc cagatcttca
      421 atgtcggcgc cgaacgcatg ctcgggtata ccgccgccga ggtaatgggc aaaatcacgc
      481 cggcggacat ttccgacccg caggaactga tcgcgcgcgc caaggaactc agtgccgaac
      541 tcgagacccc gatagcgccg ggcttcgagg cactggtgtt caaggcctcg cgcgccatcg
      601 aggacatcta cgagctgact tatattcgca aggacggcag ccgcttcccg gcggtcgttt
      661 cggtcacggc gctgcgcgat gcgcagaaca ccatcatcgg ctatctgctg atcggcaccg
      721 acaacaccgc gcgcaaacga gcggaggagg ccctcctcaa ggcgggggcc ctgcagagcg
      781 ccatattcaa cagcgccaat ttctcgagca tcgccaccga cgccaacggc gtcattcaga
      841 tcttcaacgt cggcgccgaa cgcatgctcg gctatacggc cgccgaggtg atgaacaaga
      901 tcaccccggc ggacatttcc gatccgcagg agatgattgc gcgcgccaaa gccttgagcg
      961 ccgaactcga gaccccgatt gcgcccggtt tcgaggcact ggtcttcaaa gcctcgcgcg
     1021 gcatcgagga catctatgag ctgacatata tccgcaagga cggcagccgc tttccggcgg
     1081 tcgtttctgt taccgcgctg cgcgatgcgc agagcaccat catcggctac ctgctgatcg
     1141 gtaccgacaa caccgctcgg cagcaggtcg aagaggaacg caagaaactc gatcagcggc
     1201 tgcgcgatca gcacttctac acgcgctcgc tcatcgaatc caacatcgat gccctgatga
     1261 ccacggatcc ccgcggcatc gtcaccgacg tcaataagca gatggaggcg ctgaccggcc
     1321 gcacgcgcga tgaactgatc ggtgcgccat tcaagaacta tttcaccgat ccgaacctgg
     1381 cggaggcggc catctgccgc gtgctggcgg aaggtaaagt caccaactac gagctgacgg
     1441 cgcgctcgct ggagggaaag cttactgtcg tgtcttataa tgcgaccaca ttccacgatc
     1501 gcgaccgggt tctgcagggc gtcttcgccg cggcgcgaga cattacggag cgcaagcaat
     1561 acgaggaatc cctgcgtgag gctacgcacc gagcagaaca ggccaatcgc gccaagagtg
     1621 acttcctcgc caacatgagt cacgagatcc gcacgccgat gaacgcggtg atcggtctct
     1681 cctacctgct tggacagacc tcccttgacg agcagcagct ggcgtttctg gccaaggtca
     1741 agctcgccag caagtcgctg ctcgtggtgc tgaacaatgt actcgacctg tcgaaaatcg
     1801 aagcgggcga gttgatcgtc gagcgcgccc ccttcagtct gcgcagcctg ttgaaggaac
     1861 tgaccgacgt gatcgccgtg caggcggacg ccgaaggaat cggcttcgag atcgacctgc
     1921 cggacgaggt gccatcggcg ctcgagggag acgccactcg tctgagccag atcctgacca
     1981 acttgctttc caacgcaatc aagtttaccg agcgcggtgg cgtgatgctg caagtccgcc
     2041 tcctgcctgc cacgtccgaa cgcgtgacgc tgggcttcgt cgtgcgagac acaggcatcg
     2101 gcattgcgcc ggaaatgcag gcgcgcctgt tcgcgccatt tgctcaggcc gatgcatcga
     2161 tcacgcggcg cttcggcggc acgggactcg gattgtccat cgtcaagcgt ttggtcggcc
     2221 taatgggcgg gcggatggaa ctgcacagca ctcccggtgt cggcagcgaa ttcaagatcg
     2281 agctggattt ttggctggct tcgccggagg tgctggctcg tctagatgcg tctccaaacg
     2341 agcccggcaa taactcgctg gcgggtgtgc gggtgcttgt cgtcgacgac agcgacgtca
     2401 acctggatgt gacgaagcgc atcctcgaat cgaagggcgc acaagtgtgg cttgcccgta
     2461 acgggcagca ggcgttcgaa ctgattcagg ccgctcctca tgccgttgac gtggtcctca
     2521 tggatgttca gatgccgata ctcgacggtc atggtgcaac acgtcgaatt cggctcgaat
     2581 tgggtttgac gaaattgccg atcatcgcgc tgacggccgg cgcgctcagc agcgagcgcc
     2641 tgagggccac cgttgccgga atggatgact atctcatcaa gccgttcgac ccttctgctt
     2701 tggtgtggag catcctgcgc catgtcaagc ctggcggcag cccgccgtcg cggcagaccg
     2761 acggggcgag tcagacgcct gcgccagggg cgcaaccgtg gccggagatc gagggcattg
     2821 actcgatcga agctcgcgtg cacgtgaccg gcgaccttcg catgttccag gcaatgctcg
     2881 aacgtctgct cgatgaattt tcggacgtga cgatcccggc gccgatggtc gacccgagtg
     2941 cgctcgccgt tcaagccgga cgcatgcaca aactcacggg cagcgcgggc acccttgggg
     3001 ccacggcgat ccagcagctt gcaggcgaag cggaggcggc ctataccgct ggtgaggttg
     3061 accgagccgc caacctcgcg gcgaggcttg ccgggctgtt gcgggggctc cgccggagcg
     3121 cggcagcggc gcttgcggca tacccggccg agaccaaaga agaagaaccg ttcgccggcg
     3181 cagtgatcga gccacgggag gtgttcgatc tcatcgatct cctgcgtcac cagaaccttg
     3241 cc
//
LOCUS       DATGEM010000416         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1291265, whole genome shotgun sequence.
ACCESSION   DATGEM010000416 DATGEM010000000
VERSION     DATGEM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291265"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="VKQ27_13320"
     CDS             complement(<1..1633)
                     /locus_tag="VKQ27_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY89956.1"
                     /translation="MSPSRPFILRPIATSLLMIGIVLVGVVAFRQLPVSALPQVDYPT
                     IQVVTFYPGASPDVMASSVTAPLERQFGQVPGLNQMTSISSEGSSIVTLQFTLDLNID
                     VAEQEVQAAINSAGTYLPRDLPNPPIYSKTNPADTPILTLALTSGALPLSKVEDLADT
                     RLAQKISQLPGVGLVSISGGQKPAVRVQANPTALSSYGLSLEQLRASLAAANVNQAKG
                     SFDGQRQSYTIDANDQLMSSDQYRPLVIAYRNGAPVKLSDVATVIDDTENVRQAAWMN
                     HDPAVIMNIQRQPGANIIAVVDRIKKLLPTLRSSLPSAVKLDILTDRTVTIRASVADV
                     EFELMLTVALVVMVMFLFLRSIAATIIPSVAVPLSVVGTFGVMYLLGYSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEEGERPLQAALRGAEQIGFTIISLTISLIAVLIPLL
                     FMGDIVGRLFREFAVTLSVTILVSAIVSLTLTPMMCSRLLRHKPESQRGRLYRLSEQG
                     FNSVINFYGRTLRVVLRYQGITLLIAVATLVATIYQ"
     gene            complement(1630..2880)
                     /locus_tag="VKQ27_13325"
     CDS             complement(1630..2880)
                     /locus_tag="VKQ27_13325"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HLY89957.1"
                     /translation="MNIETTEHPRISSTPLPHDQPKPGRPHKSHTWLWLLVLCLVGAG
                     AYYFLAPHFRQAQVKGGKAGGQKGGPRAVPVVVTSARKGDMKIYLDGLGSVLAFNTVT
                     VRTRVDGQLDKVAFEEGQIVHQGDLLVEIDPRPFQVQLESGQGQMAKDLALLNNAKVD
                     LERYRVLLAQDAVPKQQLDTQAALVRQYEGAIKADQSIIDNARLQLVYSRITAPITGR
                     IGLRLVDAGNMVHATDTNGLAVITQLQPIAVIFTIPEQYVNQITKNLRSGRRVIVEAD
                     GPDKKNLATGYLLTIDNQIDPSTGTIKCKAVFDNKNNELFPNQFVNARLLIDTLRETT
                     IVPTAAVQRGPDSAFVYVVKPDSTVEVRNITPGPVEGDAASVATGLAAGDRVVIDGVD
                     KLQPGATVTTGGKRAGRAGKKAGK"
     gene            complement(2992..3636)
                     /locus_tag="VKQ27_13330"
     CDS             complement(2992..3636)
                     /locus_tag="VKQ27_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY89958.1"
                     /translation="MPPEQPLPIIRVLCADDHPLVRKGIASILANEADTKLVAEASNG
                     REAVELFRLHRPDVTLLDLRMPELDGVAATKAILAEFPDARLIALTSYDGDQEIYQAL
                     EAGVRGYLLKEMVHTEVLRAIRRVHAGKRLIPSEVSERLSEYFPQMALTPRETEVLAF
                     VAEGLGNKEIADRLGTAAGTVKNQIQTILAKLKASDRTQAVTIAIRRGILHLNS"
BASE COUNT          679 a         1173 c         1259 g          750 t
ORIGIN      
        1 actggtagat agtggcgacc agggttgcga cggcgatcag cagcgtgatg ccctggtagc
       61 gcagcactac gcgcagcgta cggccgtaga aattgatgac ggagttaaag ccctgctcgg
      121 aaaggcggta gaggcggccc cgctgcgatt ccggcttgtg gcgcaggagg cgcgagcaca
      181 tcataggcgt aagggttagt gagacgatgg cggaaaccag gatagtcacg ctcagcgtca
      241 cggcgaactc tcggaacagg cggcccacaa tatcgcccat gaacagcagt gggatcagca
      301 ccgcgatcag ggagatggtg agcgaaatga tggtgaaccc gatttgctcg gcgcctcgca
      361 gcgcggcctg tagcggccgt tcaccctctt cgatgtagcg cgcgatgttc tcgatcatca
      421 cgatggcgtc gtctaccacg aagccggtcg aaatggtaag ggccatcaag gtcaaattgt
      481 tgaggctgta gcccagcagg tacatcacgc cgaaggtgcc cacaaccgac agcggcacgg
      541 ccacgctggg gattatggtc gcggcgatgc tacggaggaa caggaacatg accatcacca
      601 ccagcgcaac ggtgagcatc agctcgaact cgacgtcggc taccgaggcg cgaatggtca
      661 ccgtgcggtc ggtgaggatg tcgagcttca cggccgacgg cagtgagctg cggagcgtcg
      721 gcagcagctt cttgatgcgg tccaccacgg cgatgatgtt ggcgcccggc tggcgctgga
      781 tattcatgat gaccgccgga tcgtgattca tccacgccgc ctgccggacg ttttcggtgt
      841 cgtcgatcac cgtcgccacg tcggacagtt tgactggagc gccgttgcgg taggctatga
      901 ccagcggccg gtactggtcg ctcgacatga gctggtcatt cgcatcgatg gtgtaggact
      961 ggcgctgccc gtcgaagctg cctttcgcct ggttgacgtt ggccgccgcc agtgaggccc
     1021 gcagttgctc caggctcagc ccataggagg agagcgccgt cggattcgcc tgcacccgca
     1081 ccgcaggctt ctggccgccg ctgatgctca ccaggcctac gccgggcagt tgcgagatct
     1141 tctgcgccag acgggtatcg gcgaggtctt ccaccttcga cagcggcagc gccccggagg
     1201 taagcgccag cgtcaggatg ggcgtgtcgg cgggattagt cttgctatag atgggcggat
     1261 tcggcaaatc gcgcggcaga tacgtcccgg cggagttgat ggccgcctgt acctcctgct
     1321 cggcaacgtc gatgttcaga tccagagtga attgcagggt gacgatcgag ctgccttccg
     1381 agctgatcga cgtcatctgg ttcagcccag gcacctggcc aaactggcgc tcgagcggcg
     1441 cggtcaccga agacgccatg acgtcggggc tcgcgccggg ataaaaagta accacctgga
     1501 tggtgggata atccacctgc ggcagggccg agacgggcaa ctggcggaac gccaccacgc
     1561 ccaccagcac tatgccgatc atcaggagcg aggtcgcgat cggccttaga atgaagggcc
     1621 gcgaagggct cacttgcccg ccttcttccc cgcccggccc gcgcgctttc cacccgtcgt
     1681 gacggtggcc cccggctgga gcttgtcgac gccatcgatg acgacacggt ctccggccgc
     1741 gaggccggtc gctaccgaag ccgcatcccc ttcgaccggg ccgggcgtga tgtttctcac
     1801 ctcgaccgtg ctgtccggct tcaccacgta aacgaacgcg gagtccggac cgcgctgcac
     1861 cgcggcggtg ggaacgatgg tggtttcgcg cagcgtgtcg atcagcagcc gggcgttgac
     1921 gaactggttg ggaaacagct cgttgttctt gttgtcgaat accgccttgc acttgatcgt
     1981 tccagtgctc gggtcgattt gattgtcgat agtcagcaga taacccgtgg caaggttctt
     2041 cttgtccggg ccatctgcct ccacaatgac ccgcctgccg gaccgcaggt ttttcgtgat
     2101 ctggttgaca tactgttcgg ggatcgtgaa gatcacggcg atgggctgca attgggtgat
     2161 caccgccagg ccgttggtgt cggtcgcgtg caccatgttt cccgcgtcca ccaggcgcag
     2221 gccgattctg ccggtgattg gcgcggtgat tctgctgtaa accagttgca gccgggcgtt
     2281 gtcgataatg ctctggtcgg ctttgatggc gccttcatat tgcctcacca gtgcagcctg
     2341 ggtgtcgagc tgctgcttcg gcacggcgtc ttgcgccagc aggacccggt aacgctccag
     2401 gtccactttg gcgttgttga gcagcgccag gtccttcgcc atctggcctt ggccggactc
     2461 gagctgcacc tggaagggcc tggggtcgat ctctaccagg aggtcgcctt gatgcacgat
     2521 ctgtccctct tcaaacgcca ccttgtcgag ctgcccgtcc actcgcgtgc ggaccgtgac
     2581 ggtattgaac gccagcaccg aaccaagccc gtcgaggtag atcttcatgt cgcccttgcg
     2641 tgcgcttgtg acgacgacgg gtacggcgcg cggcccgcct ttctggcctc ctgccttgcc
     2701 gcctttgacc tgggcctggc ggaagtgggg agcgaggaaa tagtacgctc cggctcccac
     2761 caggcagagc accagcagcc agagccacgt gtggctctta tgcggacggc cgggcttcgg
     2821 ctgatcgtga ggcagggggg tcgaggagat tcgcgggtgc tcggtggttt cgatattcat
     2881 ctgcacgagt ccttccaggg caatgattcc aggatcggag gtgctgcaag tgggggtaag
     2941 tgcaggctgg cacaatgcgc cgtatgccga tcagcctatt cgtacggaaa attacgaatt
     3001 cagatgcaag atgccacggc ggatggcaat ggtcacggcc tgcgtgcgat ccgaggcttt
     3061 cagcttggcg agaatggtct ggatttgatt ctttacggtg cccgcagccg tgcccaggcg
     3121 gtccgcaatt tccttgttgc cgagaccctc cgccacgaac gccagcacct cggtttcccg
     3181 cggggtaagg gccatctgag ggaagtactc acttaaccgc tcggaaacct ccgagggtat
     3241 cagcctcttg cccgcatgca cgcggcggat cgcccgcagc acttcggtgt ggaccatctc
     3301 cttcagcaga tagccgcgaa cgccggcctc gagcgcctgg tagatttcct ggtctccgtc
     3361 atagctggtg agcgcgataa ggcgggcgtc cgggaactcg gcgagaatcg ccttggtggc
     3421 ggcgacgcca tccagttccg gcatgcggag atccaacagc gtcacgtcgg ggcggtgtag
     3481 ccggaacagc tccacggctt cgcgtccgtt ggaagcttcg gcgaccagct tggtgtcggc
     3541 ttcgttggcc aggatggaag cgatgccctt gcgcaccagc gggtggtcat cggcgcaaag
     3601 cacgcggatg atcgggagtg gctgttcggg aggcatttag gaatccgtcg caaccggccc
     3661 ggcgcttgaa gccacggcca cgccggccgc tttcctcgcc ggcagcacga cccgcaccgt
     3721 agtgccatga ccgggctggc tttcgagctg caattgcgcg ccgatttgcg ccgcccgttc
     3781 tctcatgccc gtgaggccgt agtggccagg cgcggcgaca gatccattgc cggccggcat
     3841 gcccgaacca tcatccttca c
//
LOCUS       DATGEM010000417         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_517667, whole genome shotgun sequence.
ACCESSION   DATGEM010000417 DATGEM010000000
VERSION     DATGEM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517667"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VKQ27_13335"
     CDS             complement(<1..1515)
                     /locus_tag="VKQ27_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY89959.1"
                     /translation="MPSSDRPDDINRAAITVCVILATLMQSLDTTIANVALPYMQGSV
                     SATQDQIDWVLTSYIVAAAIMTPPTGFLAGRFGLKRLFLISVAGFTVASMLCGMAQSL
                     VQIVLFRVLQGLFGAALVPLSQTVLFNINSKERQGAAMALWGVAVMAGPVLGPVIGGW
                     LTQVYSWRYVFYINLPIGILAFIGITTFLPETARQSGAKLDWFGFGTLSLAVGALQVL
                     LDRGEQLDWFDSLEIVIEAIVAGSAFYLFLVHTFTAKAPFVRPSLFRDRNFAAGVLFV
                     AIIGLTYYASLALQPSYLQQLLNYPIISAGLVMGPRGVGTMAVMMLVGKLIGRIDTRI
                     LLSIGLGLTAWSFSEMTGWTPDVSRTAIVVIGVVQGAGLGFLFVPLSVATLSTLSSEQ
                     RAEGAGLYSLSRNIGSSIGISVVSGLLTRNTQVNHAAIAQHVTAVNRMFDVRAIARFW
                     SPITATGRAALDAVITRQAQIIAYIDDYKLLMIATIAVIPLLLAFKRPSREGAPQ"
     gene            complement(1515..>2327)
                     /locus_tag="VKQ27_13340"
     CDS             complement(1515..>2327)
                     /locus_tag="VKQ27_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLY89960.1"
                     /translation="QRAEAQLGMVGDAINALKANYRDVQAQIKQAQNDVGYFNSEFNR
                     QQGLLNAHVASQSAFDLARRNLQTAEQKLASLRQQLAAVVANLGGDPESPNDANPRYR
                     DALAQRDEAARQLAHTIVKAPFAGIATNVPQIAPGKYLQASETAFYLVSSDQIWVDAN
                     PKETKLTYVRPGQPATVTVDSYPNLSWRGTVETISPAAAQEFSLLPAQNTSGNWVKVV
                     QRIPMRVRVDTSDGNLPPLRAGMSVEVEVDTGHARGLPYFLTTLFGHAPGRP"
BASE COUNT          475 a          682 c          761 g          409 t
ORIGIN      
        1 ctgcggagcg ccttcgcggg atggcctctt gaaggcgaga agcagcggaa tgacggcgat
       61 cgtcgcgatc atcaggagct tgtaatcgtc gatataggcg atgatctgcg cctgccgcgt
      121 gatgacggca tcgagtgccg cccggcctgt ggcggtgatc gggctccaga accgagcgat
      181 cgcacgaacg tcgaacatac gattgaccgc cgtcacatgt tgcgcgatgg cggcatggtt
      241 cacctgtgtg ttccgggtca acaggccgct tacgaccgag atgccgatgc tggagccgat
      301 attgcgcgac aggctgtaga gaccggcgcc ttccgcccgt tgctcggatg acagggtgga
      361 tagggtggcc acgctcagcg gcacgaagag gaagccaagg ccggcgccct ggacgacgcc
      421 gatgacgacg atggccgtcc gcgaaacatc cggcgtccag ccggtcattt cggagaatga
      481 ccaagccgtg agccccaggc cgatggacag aaggatgcgc gtatcgatgc gcccgatcag
      541 ttttccgacg agcatcatga cggccatggt tccaacgccg cggggtccca tgacaagacc
      601 cgcgctgatg atcggatagt tcaacagctg ctgtagatag gagggctgca gggcgagcga
      661 agcataatag gtgaggccga tgatcgcgac gaagagaacg ccggccgcga aattgcgatc
      721 gcgaaacagc gacggcctca caaagggtgc tttcgcggtg aaggtgtgga ccaggaacag
      781 gtagaacgcc gagccggcga cgatggcctc gatcacgatt tccagggagt cgaaccagtc
      841 gagttgttcg ccgcgatcca gcagaacctg cagggcgccg accgcgaggc tcagagtgcc
      901 gaagccgaac caatccaact tggccccgct ttgccgcgcc gtctcgggca ggaaggtggt
      961 gatgccgatg aaggcgagga tgccgatcgg caagttgatg taaaacacat agcgccagct
     1021 atagacctgt gtcagccagc cgccgataac gggtccgaga acgggaccgg ccatcaccgc
     1081 gacgccccag agggccatgg cggctccctg gcgctccttc gaatttatgt tgaacaagac
     1141 ggtttgtgag agagggacca gcgccgctcc gaacaaaccc tgcaggaccc gaaacagcac
     1201 gatctggacc agggattgcg ccatgccgca cagcatcgaa gcgaccgtga agccggccac
     1261 cgaaataagg aagaggcgct tcaatccgaa acgtccggca aggaagccgg tcggcggcgt
     1321 catgatcgcg gcggcgacga tataggaggt cagcacccaa tcgatctggt cctgagtggc
     1381 cgaaacactc ccctgcatat aaggcagcgc cacattggcg atggtcgtgt cgagcgactg
     1441 catgagtgtc gccaggatta cgcagaccgt gatcgccgcg cggttgatat catccggtct
     1501 atccgacgac ggcatcacgg cctcccgggg gcatgaccga acagcgtggt caagaaatac
     1561 ggcaaacctc gcgcatggcc ggtgtcgacc tcaacctcga cgctcattcc cgcgcgcaaa
     1621 ggcggcaggt tcccgtcgct ggtgtcgacg cgcacgcgca tcgggatgcg ctggacgacc
     1681 ttcacccagt tgccgctggt attttgtgca ggcagcagcg agaactcctg cgccgccgcc
     1741 gggctgatcg tttcgacggt tccgcgccag gaaaggttcg gatagctatc cacggtgaca
     1801 gtggctggct gccccggacg cacataggtc agcttggtct ccttgggatt cgcgtcaacc
     1861 cagatctgat ccgaagagac gagataaaag gctgtttccg acgcctgaag atatttgccg
     1921 ggtgcaatct gcggcacgtt cgttgctatt ccggcgaagg gggctttgac gatcgtgtgg
     1981 gcaagctggc gggcggcctc gtcgcgctgc gccagtgcgt cgcggtagcg cgggttggcg
     2041 tcgttcggac tttccggatc gccgcccaaa ttggccacca ccgctgccag ctgttgccga
     2101 agagaggcga gcttctgctc ggcggtttgc aaattgcgcc gcgccaaatc gaaagccgat
     2161 tgggatgcga catgcgcgtt caggaggccc tgctgacggt tgaattctga attgaaatag
     2221 ccgacgtcgt tttgcgcctg cttgatttgc gcctggacgt cccggtagtt cgccttgagc
     2281 gcgttgatcg cgtccccgac catgccgagt tgcgcctcgg cgcgttg
//
LOCUS       DATGEM010000418         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_584116, whole genome shotgun sequence.
ACCESSION   DATGEM010000418 DATGEM010000000
VERSION     DATGEM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584116"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..242)
                     /locus_tag="VKQ27_13345"
     CDS             complement(66..242)
                     /locus_tag="VKQ27_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89961.1"
                     /translation="MTTLLQQIEELRAELRGSYMSRRERTKAEAELARMVAEQAALDQ
                     ACDRALAAYRQQWR"
     gene            complement(461..634)
                     /locus_tag="VKQ27_13350"
     CDS             complement(461..634)
                     /locus_tag="VKQ27_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89962.1"
                     /translation="MLTIYDQIQELRAEITHCVMTRRERAEAKAELQRLIAEQEKLDR
                     AFDMVFAAEAPPD"
     gene            complement(901..1377)
                     /locus_tag="VKQ27_13355"
     CDS             complement(901..1377)
                     /locus_tag="VKQ27_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89963.1"
                     /translation="MTHESRRSEPSASHEKRRQGTEQRLRAALARLNSGTPIHPSLQG
                     CAYQMTISVLAREAGVGRNTIYTNHRSILDELSGANRRTTLPSRPAPTQKTTELTALI
                     EQLRKQKRQLATENASLLKRAIDAEKAVDRLRKHNAKIVRELAATRQPIALAGGQT"
     gene            complement(1367..>2637)
                     /locus_tag="VKQ27_13360"
     CDS             complement(1367..>2637)
                     /locus_tag="VKQ27_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89964.1"
                     /translation="PLVCAAIRLLDGPADDIIALRDRAQFACDDATARALDAGAAKAS
                     VRAALAGFIVSTPAGENKPWRTEPIDDLAAVGFLVNRLYDACFVVIAYLVGARVSEIL
                     GLRAACIEELPSADGTETFAYLSGRIYKTADSPGGQPHRWVAPVPVIRAVAVLERLSE
                     PLRRHTGQDELWLMPAVNRDCGIGIRRSANLIDHLNGPFAAFIGLPAHNGRPWHLTTH
                     QGRKTFARFVGRRDRTGLHALAAHLGHVTRIMTDTSYVGTDFELGDLIDAEAIEDTRS
                     ALEDLLTATRLAGKAGRMIAERSRFRGRTRDGDVREYVDFILRETDMRLGACDWGYCV
                     YRRESSACLGGDNGPNPVLRTQSTCAGCANFAVSEKHRPVWATRRQRNADLLGHPALD
                     AESHALAAARVEECDRILTELDQSRDHNDA"
BASE COUNT          452 a          823 c          862 g          500 t
ORIGIN      
        1 gtccggtcgt cggcaagtgc gatcgtttcg aacggcgcag tactggagtt gaacgcgctc
       61 atggctcacc tccactgctg gcggtaagcg gcgagcgcgc ggtcgcacgc ttggtcgagg
      121 gcggcttgtt cggccaccat ccgcgccagc tcggcctcgg ccttcgtgcg ctcgcgtcgg
      181 gacatgtacg atccccggag ctcagcccga agttcttcga tctgctgcaa aagcgtagtc
      241 atgatcctct ccgtcagttt cgaaagacgg agaggggcgc gtcatgccgg atcgggccgg
      301 gtcagggacg ccgaaggcgc cgcgcagcgg ggcgcggggg agccgatttt gccgggcgtt
      361 ggtaacattt cgcgcgtagc gctccatgcg cccggctaaa ttgggggttg accgcgctta
      421 ggtcacacgt caactgtcct gttaggcaag ttgcgccgcc tcagtcgggc ggcgcttcgg
      481 cggcgaagac catgtcgaac gcgcgatcga gcttctcctg ctccgcgatg agcctctgca
      541 gctcggcctt cgcttcggcg cgctcacgcc gcgtcatcac acaatgcgtg atctcggcgc
      601 gcaattcctg gatttggtca tagatcgtaa gcatttgcct ctcctctgtt ttgaagacga
      661 gaggcgctcg cgacgctgga ttggcccggg tcgaggatcg ccgaaggcga ccggcttgcc
      721 ggcgagtgga ggaaccgata tgcgcagcat attggagggg ccgctcgtcc tcgaggcgag
      781 ccaatccagc gtggcacaat gggaagtctg aagagacaga cggtttttca ggggcttcga
      841 cgagctgaag ccgcccagcc gagacgatga tccgtcctgg tcacccgccc gagaatgccg
      901 tcaggtttgt cctcccgcca gcgcgatcgg ttgacgtgtg gcagccagtt cacggacgat
      961 cttggcattg tgcttcctca ggcggtcgac cgccttctcg gcatcgatcg cacgcttgag
     1021 gagcgacgca ttctcggttg caagctgccg tttttgcttc cgcaactgct cgatcagggc
     1081 ggtaagctcg gttgtttttt gagtgggggc aggccggctc ggcagggtgg tcctgcggtt
     1141 ggcgccgctc agttcatcaa ggatcgagcg gtggttggtg tagatcgtgt tgcgcccgac
     1201 cccggcttcc cgcgccaaca ccgagattgt catctgataa gcgcacccct gcaaggaagg
     1261 atggatgggc gtcccggagt tgagccgcgc caatgcggcc cgaagacgtt gttcggtacc
     1321 ctggcggcgc ttctcgtgcg aagccgacgg ctcgctcctt cgtgattcat gcgtcattgt
     1381 gatcccggct ttgatcgagt tcggtgagaa tgcggtcgca ttcctcgacg cgcgccgctg
     1441 ccaaagcatg gctctccgcg tcgagggcgg gatggccgag cagatcggca ttgcgctgac
     1501 gccgcgtcgc ccaaaccggg cgatgcttct cgctcactgc gaagttggcg cagccggcgc
     1561 aggtactttg tgtgcgcagc acgggattcg ggccgttgtc gccgccgaga caggccgagc
     1621 tttcacgccg atagacgcaa tagccccagt cgcatgcgcc gagccgcata tcggtctcgc
     1681 gcagtataaa atcgacgtat tctcgcacgt caccgtcgcg cgtgcggccc ctgaagcggc
     1741 tgcgctcggc gatcatgcgg cccgctttcc cagcaagccg ggtggcggtc agcaggtcct
     1801 cgagcgcgct tcgcgtatcc tcgatcgcct cggcatcgat caagtccccg agctcgaagt
     1861 ctgtgccgac atagctcgtg tcggtcatga tacgggtaac atggccgaga tgggcggcca
     1921 gcgcgtgcaa ccccgtccga tcgcgccgac cgacgaagcg cgcgaaggtc ttgcgtccct
     1981 gatgcgtggt cagatgccat ggtcggccgt tgtgggccgg caaaccgata aacgcggcga
     2041 atggaccatt gaggtggtca atcaggttcg cggatcgccg gatgccgatc ccacagtcgc
     2101 gattcacagc cggcatcaac cagagctcgt cctgccctgt atgccgacgc agcggctccg
     2161 acaatcgttc cagtaccgca acggcgcgta tgacgggcac cggcgccacc cagcgatgag
     2221 gctgtccgcc gggactgtcg gctgtcttat atatacgacc gctcaggtac gcgaaggtct
     2281 ctgttccatc ggccgaggga agctcctcga tacatgcggc ccgaaggccg aggatctccg
     2341 acacccgcgc gccaaccagg taggcgatga cgacaaaaca cgcatcatat agacggttca
     2401 caaggaagcc gacggccgcc agatcatcga tcggttcggt tcgccaaggc ttgttctcgc
     2461 ctgcgggcgt cgagacgatg aagcccgcca gggcggcgcg aacagatgcc tttgccgcac
     2521 ccgcatcaag cgcccgtgcg gtcgcatcat cgcaggcaaa ttgcgctcgg tcgcgcaacg
     2581 cgatgatatc gtcggcggga ccgtcgagaa gccggatggc ggcgcacacc agcggga
//
LOCUS       DATGEM010000419        10181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1197642, whole genome shotgun sequence.
ACCESSION   DATGEM010000419 DATGEM010000000
VERSION     DATGEM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197642"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VKQ27_13365"
     CDS             <1..380
                     /locus_tag="VKQ27_13365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HLY89965.1"
                     /translation="GRTLAAGHRAVVLCTDVERVAALDAALWQCAEPDWLPHGSATDG
                     DADLQPIWLTAEESAPNGARFLFLLDGARSEHPEKFDRVFDLFDGKDEAAVQAARERW
                     RAAKEAGHALTYWQQGVKGWEKA"
     gene            complement(358..882)
                     /locus_tag="VKQ27_13370"
     CDS             complement(358..882)
                     /locus_tag="VKQ27_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89966.1"
                     /translation="MIYMVEMALLDTARRAEWEAWYTAHQHHLLSIPGIRASQRFECI
                     HTAASPFVALHEVDGPEVFTSDAYRNSAGPSNTGEWQTKMSNWHRNLLRGLDHTPNVP
                     PDARLVIVERGAEGSLPRDAIVRWLESVGLDQSVRRRGIAVMPPSQADLLAGTPGIRV
                     LKPLGERLTPSPSP"
     gene            complement(895..2676)
                     /locus_tag="VKQ27_13375"
     CDS             complement(895..2676)
                     /locus_tag="VKQ27_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY89967.1"
                     /translation="MTAAITSHTTRALLIRLLREYILHYKARLVWVLALSGAMAGATA
                     LYPLVLRYAGILLTAGDPRLAWQIPLAVVGVTALKGITQYLQTVQAQQFVLLIIRDLQ
                     QRMFAHLTRADLARIEREAPAQLATRFTTDATAIGGALGRVVSGVADLVTVVGLVASM
                     IYMDWVLSLVAALLYPLAAVPIQRLGKRVRRYSGGMQAQMGETAATLNESFAQARTVR
                     AYRLEDAETKRANQAFDALYRTFLGITRVRSRVEPFLEVLGGVAVAAVIVFEAWRASG
                     SSHTLGDFGGFVGALLLAARPLRALGSLNAALQEGLSGLSRVFTVIDETPHILDAPDA
                     KQLPGGHGHVRFAHVAFRYPDGRAGLRDLSFEALPGQTVALVGPSGAGKSTALALIPR
                     LHDVSAGAVTIDGADVREVTLVSLRDSIAYVAQDPLLFDDTVAANIRMGRPGTDEHTV
                     EAAARAARAADFIAALPEGYQTRVGPGGQRLSGGQRQRIALARALLRDPRVLLLDEAT
                     SALDAENEAAVQAALTRLRAGRTTIIIAHRLSTVRDADVVVAMEDGQALEIGSHDALL
                     AANGLYARLVRSQSLERDTSLTWQAIG"
     gene            complement(2782..5721)
                     /locus_tag="VKQ27_13380"
     CDS             complement(2782..5721)
                     /locus_tag="VKQ27_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HLY89968.1"
                     /translation="MATNSWSADVSGAWTDASKWSSVAVPGARDNALIAEPLDNPDQP
                     AGLIGTIEGFTHGATIDLGLAASPVSYSGGDLKLPATGSQTLDLHIAGPYSLANLIAV
                     TQDATTAIEVNGSVASTPVLQGAGNTITYVASGTAVAIAPGLAISDPGSAALTGATVS
                     VSSGAYPEDGDVLSAITTDTGILASFNATTEVLTLSGLDTLANYQSVLRSVAFGSGAA
                     DPTMATSNPSRTISWVINDGTATSAPVTSIVNLQPPPRVLTWVGTQGSSIFTAANWND
                     TTNSVNPASLAPDAADSALFEIDGGTITGAGTIGSIAFACGNDWQLGSGTSLNAVDGI
                     GDSGGVTLVSDATVISQGNLDSVSAFDGNTASLTISGTNGLWHSLGALIVGEQSASGA
                     LTIENRGALISGGLVVAQAGGAGTVAVASNGSLQVASGPFVIGAEGRGSLVIASGGDA
                     SVAGDASIAGEGADGSSASVTGAGSTWQIAGALQLGNGAAGALDVADGGTVTAAALDA
                     GVQNVGIRQGVGQIDVIGSGSQINIGGNAIIGDAGSGTLSILGGGTLTATNLVIGNAT
                     GDGIASVSGNGSLLDLSGTLSIGVTLGIGELTIGSGATVAADTAVMQNHVMNQGGTFA
                     ASQIEITSGATLDGFGIFGSPNALITNDGTFSTGGGVAVGNGSIAGAGAVLIGDGSTL
                     DLTGGVVGSQSVAFTTSAGALIVRHPGDFHGVITQFAAGDQIFVEAPFAGSFRQQGSI
                     VQVLTAGDVVGALDFATTALATEAATTPGALVCDIVPCFTAGTRIMTERGEVAVEDLF
                     EGEYVEVLGGRTGRIIWIGHRTIDCTKHPSPEKVWPVRVRTGAFGPGKPCRDLWLSPD
                     HAVFIAEVLIPVKYLTNGASIAQVAVRTITYYHVELRRHAVLLAEALPVESYLDTGDR
                     TNFADGGGVMTLHPDFSSRVRDAEGCAPLVVTGHDLNAARLMVNALAFLPHGGHAPMG
                     RPYAPPH"
     gene            complement(5843..7381)
                     /locus_tag="VKQ27_13385"
     CDS             complement(5843..7381)
                     /locus_tag="VKQ27_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HLY89969.1"
                     /translation="MNGAESLVHTLLRSGVDTCFSNPGTSEMHFVAALDRVPGMRCVL
                     GLFEGVVTGAADGYARMSGKPAATLLHCGPGLANGLANLHNARRAQTPIVNIAGDQAT
                     YHRPNDAPLTADTEGWARGASAWVRTATSSATVGADAAVAVQAARTHPGQIATLILPS
                     DTSWDEGGVAAEALSVPPRPQSDPAQVRNAAEILRRGLPSLIVLGGEAVRADALESAH
                     RIAAATGARLIAQGANARISRGRGRVPVDRIPYVVDTAVKALAGTRHVILCGLRKPVT
                     FFAYPNKPTSPIPTDADIHVLARPDQDAAEALARLADELGAPSAAIPDPGPRPEPARG
                     KLTPESVAATLAALLPEQAVITDESVTFGRGFFPYTYAAEPHDWLNSTGGAIGCGPPL
                     ATGAAVGAPGRRVVALQADGSAMYTLQALWTQARENLDVTTVLLSNRKYQILIGELAN
                     VGANPGRTALDMLDLGNPDLDWTKLAAGMGVEAARADSCERFADLFAQANARKGPFLI
                     ELMI"
     gene            7477..8097
                     /locus_tag="VKQ27_13390"
     CDS             7477..8097
                     /locus_tag="VKQ27_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89970.1"
                     /translation="MPVTVLYSENVYGDDSVERAVFGPDVRVFLPGPTKSLADLPDEL
                     CAQADGLMILRFRVSEGDLARFTRLRAVCRMGVGYDGIDRKAAAERQVMVLNVPDYGT
                     TEVADHAMSLALALRRGLLIHHDAQRRDPPAAWSYVRDPVVKRMGVQTFGILGLGRIG
                     TAVALRAKAFGCRVAFYDPYLPNGAELVLASGGPERWRNCCGRPIR"
     gene            8100..8486
                     /locus_tag="VKQ27_13395"
     CDS             8100..8486
                     /locus_tag="VKQ27_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY89971.1"
                     /translation="MHTPLTPETRGILGAAEMAMLPKGAVVVNTARGPIVDIDALAAA
                     LKSGHLAGVGIDVVPVEPPVDPVPELIRAYRAREPWTEGRLIITPHSAFFSSQAWEDT
                     RRLAAETMKAALLGPRPQNVIAPEGW"
     gene            8483..9406
                     /locus_tag="VKQ27_13400"
     CDS             8483..9406
                     /locus_tag="VKQ27_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HLY89972.1"
                     /translation="MTLRLLADDLTGALDSAARFVPLTGPLPIVWHDADVPGSAAIDS
                     GTRDLAEAAAGTRIAQFAPLLQGADIAFKKIDSLLRGHVALELAACMRFFDYCVLAPA
                     FPFQGRITRGGRQLFHAGDTWHDTGVTLPDMRDAESDADLDAIVADGHALTGRVLWCG
                     TGGLAGALANHRPVPRLSLPTPILALIGSDHPVSRAHVAAVPADRRAAVVACDLPPNT
                     DRLAAREHIRATFAATLRQTERPGTLFVTGGETLRDLCSELGATHLEVDGELEPGVPT
                     SILRGGSWNGQRIVSKSGAFGDAGFLARLLL"
     gene            9434..>10181
                     /locus_tag="VKQ27_13405"
     CDS             9434..>10181
                     /locus_tag="VKQ27_13405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY89973.1"
                     /translation="MRLGVMLPLTDIGGEPNALRDFALAAEELGYTNLGLPDHVLGAN
                     AATHPEVAGRNTSADMFQDPFVAFGFLAGLCRPTTEFSTQVLILAQRQTVLVAKQAAC
                     LDLLCGGRFRLGVGVGWNPVEFVGLNESFTNRGRRSAEQVVVMQKLWAEPHVVFKGKW
                     HTIDDAGINPLPVNRHIPVWFGGNVDQTLERIATIGDGWIMNAYGPGDAALAEFSKLR
                     RLTEAAGRDPAKIGIEVWTSVGAGSEADWRE"
BASE COUNT         1607 a         3170 c         3679 g         1725 t
ORIGIN      
        1 tggggcgaac actcgcggca ggccaccgcg ctgtcgtgct gtgcactgac gtggaacgtg
       61 ttgccgcgct ggacgctgcg ttatggcagt gcgccgaacc cgactggctg ccgcacggtt
      121 cggcaacgga tggcgatgcc gatctgcagc cgatctggct caccgcggaa gagtctgcgc
      181 cgaatggtgc tcgcttcctg tttctgctgg atggcgcacg aagcgaacac ccggaaaaat
      241 tcgaccgggt gttcgatctg ttcgatggca aggacgaagc cgcggtgcag gcggcgcgtg
      301 aacgctggcg cgccgcgaag gaagccgggc acgcactgac ctattggcag cagggcgtta
      361 agggctggga gaaggcgtaa gccgctcgcc gagcggcttt agcacgcgga tccctggcgt
      421 gccggccagc aggtcagcct gcgatggagg catcacggca atgccgcggc ggcgaacgct
      481 ctggtcgaga ccgacagatt ccagccagcg tacaatggca tcgcgtggca aagagccttc
      541 ggcgccacgc tcgacgatca cgaggcgtgc gtcgggcggg acgttgggtg tgtggtccag
      601 gccgcgaagc aggttgcggt gccagttgct catcttcgtc tgccactcgc cggtgttcga
      661 ggggccggca ctgttgcgat aggcgtcgct agtgaagact tccggcccat cgacctcgtg
      721 cagcgcgacg aagggggacg cggcggtatg gatgcactcg aagcgctggc tggcgcggat
      781 gccaggaatg ctcagcaggt ggtgctgatg cgcggtgtac caagcctccc attcggcccg
      841 ccgcgcagta tcgagcagcg ccatttcgac catgtagatc atatgccagc ccattcaccc
      901 aatggcttgc caagtcaagc tggtgtctcg ctccagcgat tgcgaccgca ccagccgtgc
      961 gtacagccca ttagcggcca ggagtgcgtc gtgcgagccg atctccaatg cctggccgtc
     1021 ctccatcgcg acgaccacgt cggcatcgcg caccgtggat agccgatgcg cgatgatgat
     1081 tgtcgtgcgg ccagcgcgca gccgcgtcag cgcggcctgc acggcggcct cgttctcggc
     1141 gtccagcgcg ctggtcgcct cgtcgagcag cagcacgcgc ggatcgcgta acaacgcccg
     1201 ggccagtgcg atgcgttgcc gctgtccgcc cgagagccgc tgcccacctg gcccgacgcg
     1261 cgtctgatag ccttccggca gcgccgcaat gaagtcggct gcgcgtgccg ccctggcggc
     1321 cgcctcgacc gtgtgctcat cggtgccggg ccggcccatg cggatgttgg ccgccactgt
     1381 atcgtcgaac agtagcgggt cttgggcgac ataggcgatg ctgtcgcgca ggctgaccag
     1441 tgtcacctcg cgcacgtccg cgccatcgat cgtcaccgcg cctgcgctca catcgtgcag
     1501 ccgcgggatc agggcgagcg cagtggactt cccggcgccg gaagggccga ccagcgctac
     1561 cgtttgcccg gggagtgctt cgaacgacag gtcgcgcagc ccagcccttc catcgggata
     1621 acggaatgcg acatgcgcga accgcacgtg cccgtggcct cccggcagtt gcttcgcatc
     1681 gggcgcatcc aggatgtgcg gtgtctcgtc gatcacggtg aagacccgcg aaaggcccga
     1741 aagcccctcc tgcagcgccg cgttcagtga ccccagcgcg cgtagcggtc gtgccgccag
     1801 aagcagcgcg ccgacgaagc cgccgaaatc cccaagcgtg tggctggatc ccgaggcgcg
     1861 ccaggcctcg aatacgatga ccgccgcgac cgccacgccc cccagtacct cgaggaatgg
     1921 ttcgacccgg gagcgcacac gggtgatgcc gaggaatgtc cggtacagcg cgtcgaatgc
     1981 ctggttggcg cgcttcgtct ccgcatcttc aagccgatac gctcgcaccg tccgtgcctg
     2041 cgcgaaactc tcgttgaggg tggccgcggt ctcgcccatc tgcgcctgca tgccgcccga
     2101 gtagcgccgc acgcgcttgc cgagccgctg aatcggcacc gcggcgaggg gatagagtag
     2161 agccgcgacc agtgacagga cccagtccat gtagatcatc gaggcgacca gcccgaccac
     2221 cgtcacgagg tcggccacgc cgctcaccac gcggccgagc gcgccgccga ttgcggtggc
     2281 atccgtcgtg aagcgcgtcg ccaattgcgc cggcgcttcg cgttcaatgc gcgccaggtc
     2341 cgcgcgcgtc aggtgcgcga acatgcgctg ttgcaggtcg cgaatgatca acagcacgaa
     2401 ctgctgggcc tggacggtct gcaggtattg ggtgatgcct ttcaacgcgg tcacgcccac
     2461 cacggcgagc ggaatctgcc atgccaggcg cgggtcgcct gcggtgagca ggatgcccgc
     2521 gtagcgaagc accagtgggt acagcgctgt cgcgcccgcc atcgcgccac tgagcgccag
     2581 cacccacacc agccgcgcct tgtaatggag tatgtattcg cggagcagcc ggatcagcag
     2641 cgcacgggtc gtgtgagagg tgatggcggc ggtcatgggt gagcagtcac ccggacgatc
     2701 cggccggcgc cgcgtctacc ggtcaaccga tacatccttt tccctgggcg cgggcggcgt
     2761 accgccaaag gcgcggccgg gtcaatgggg cggcgcgtaa gggcgcccca tgggggcgtg
     2821 gccaccatgt ggaagaaacg ccagtgcatt caccatcagg cgtgcggcat tgaggtcgtg
     2881 tccagtcacg acaaggggcg cgcagccttc cgcatcccgg acgcgcgagg agaagtcggg
     2941 gtgcagtgtc atcacgccgc cgccgtcggc gaagttcgtg cgatcgccgg tgtcgaggta
     3001 ggattcaacc ggcagcgctt ccgccagcaa cacggcatgc cggcggagtt ccacgtgata
     3061 gtaggtgatc gtccgcacgg ctacctgggc tatgctggcg ccattggtca ggtacttcac
     3121 cgggatcaga acttcggcga tgaacacagc gtggtccggt gacaaccaga gatcgcggca
     3181 aggcttgccg ggtccgaatg cgccggtacg cacgcgaacg ggccagactt tctctggtga
     3241 tgggtgcttc gtgcagtcga tggtgcgatg accgatccag atgatccggc cggttctccc
     3301 gccgagcacc tcgacatatt cgccctcgaa caagtcctcg acagcgactt ctccacgttc
     3361 ggtcatgatt cgcgtgccgg cggtgaagca cggcacgatg tcgcagacca acgctcctgg
     3421 ggtggtcgcg gcttcggttg cgagcgccgt ggtggcgaag tccagcgcac cgacgacgtc
     3481 tccagccgtg aggacctgaa cgattgagcc ttgctggcgg aagctgcctg cgaagggggc
     3541 ttcgacaaag atctggtcac cggcggcgaa ctgcgtgatg acaccgtgga agtctccggg
     3601 atgccgcacg atcagagcac cggccgatgt ggtgaatgca acggactggc tgcccacgac
     3661 accgccggtc aggtccagtg tcgagccgtc gccgatgagc actgcgccgg cgccggcaat
     3721 cgatccattg ccaacggcaa cgccgccgcc tgtgctgaat gtgccgtcat tggtgatcag
     3781 tgcgttgggc gagccgaaga tgccgaagcc atccagcgtg gcgcctgatg tgatctcgat
     3841 ttggctcgcg gcgaacgtgc cgccctggtt cataacgtga ttctgcataa ccgctgtatc
     3901 ggcggcgacg gttgcgcctg agccgatggt gagttcgccg atgccgagcg tgacgccgat
     3961 gctgagcgtg ccggacaggt cgagcaggct gccgttgcca gagacggagg cgataccgtc
     4021 gcctgtcgca ttgccgatga cgaggttggt ggcagtgagc gtgccgccgc cgaggatcga
     4081 cagcgtgccg ctaccagcat caccgatgat cgcattgcca ccgatgttga tctggctgcc
     4141 cgatccgatg acatcgatct gaccgacgcc ttgtcgtatg ccgacattct gcacaccggc
     4201 gtcgagtgcc gcggccgtga ccgtgccgcc atcggcgaca tcgagtgctc ctgcagcacc
     4261 gttgcctaac tggagcgcac cggcgatctg ccaggtcgaa ccagcgccgg tgactgatgc
     4321 actggagccg tcggcgcctt cgccggctat cgaggcatcg ccggcgactg acgcgtcacc
     4381 acctgatgcg atgacaagcg agccgcggcc ctccgcgcca atgacgaaag ggccggatgc
     4441 gacctgcagc gagccgttgc tggcgacggc aacggtcccg gccccgcctg cctgggcgac
     4501 cacgagcccg ccggagatga gcgcgcctcg gttctcgatg gtgagcgcgc cgctcgccga
     4561 ttgctcgccg acgatgaggg cgccaagcga gtgccacagc ccattggtgc cgctgatggt
     4621 cagcgatgcc gtgttgccgt cgaaggccga aaccgagtcc agattgccct ggctgatgac
     4681 tgtcgcatcg ctgaccagcg tgaccccacc gctatcgcca atgccatcga ctgcgttgag
     4741 cgacgtgccg ctgccaagct gccagtcatt gccgcaggcg aacgcgatgc tgccgatggt
     4801 gcctgcgccg gtgatggtgc cgccgtcgat ctcgaagagc gccgagtctg ctgcatccgg
     4861 cgcaaggctg gcaggattga cgctgttggt cgtgtcgttc cagttggcgg ccgtaaagat
     4921 gctgctgcct tgcgtgccta cccacgtcaa tacacgcggc ggcggctgga ggttgacgat
     4981 gctggtcacc ggggcagagg tggcagtgcc atcgttgatc acccaggaga tcgtgcggct
     5041 cgggttgctg gtcgccatgg tcgggtcggc cgcgccggaa ccgaaagcga cagaccgcag
     5101 cacgctttga taattggcga gcgtgtccag tccgctgagg gtcagcacct cggtcgtggc
     5161 attgaagctt gccaagatcc ctgtgtcggt ggtgatcgcc gatagaacgt cgccatcttc
     5221 gggataagcg ccgctgctga ctgatactgt tgccccggtg agtgcggcgg acccgggatc
     5281 gctgatcgcg aggcccggag cgatggcgac cgctgtgccg gatgcgacgt aggtgatggt
     5341 gttgccggcg ccttgcagga ccggcgtgga ggcaacgctg ccattgacct cgatcgccgt
     5401 ggtggcgtcc tgcgtgacgg caatgagatt ggccaggctg taagggcctg cgatgtgcag
     5461 gtcgagagtc tggctgcctg tcgcgggaag cttcagatcg ccgcccgaat agctgacagg
     5521 gctggcggca aggccgagat cgatcgtggc gccgtgggtg aacccttcga tcgttccgat
     5581 caggcccgcc ggctggtcgg ggttgtccag cggttcggcg atcagcgcgt tatctctggc
     5641 accgggcacg gctacggacg accacttcga cgcatctgtc catgcgccgg aaacgtccgc
     5701 actccaagaa ttcgtcgcca tgactgacgc tccgggcctg ccgcggcgca tacatggctg
     5761 atgaacgact tacggaaact taaggcgtcg gttcgccggc tgctagccgg cgcttgtggt
     5821 tccagatgca ggccgcgatg cgtcagatca tcagttcgat caggaagggg ccctttcggg
     5881 cgttcgcctg ggcaaacagg tcggcgaagc gctcgcagct atccgcgcgg gcggcttcga
     5941 cgcccatgcc ggcggcgagc ttcgtccagt ccaggtcggg gttcccgagg tcgagcatat
     6001 cgagcgcggt gcggccgggg ttggccccga cgttggccag ttctccgatc aggatctgat
     6061 acttgcggtt ggatagcagg acagtggtca cgtccaggtt ttcgcgcgcc tgggtccaga
     6121 gggcctgaag cgtgtacatg gcagagccgt cggcctggag tgcgaccacg cggcggccgg
     6181 gtgcgccgac ggccgcgccg gtggcgagcg gcggcccgca gccaatcgcg ccgcccgttg
     6241 agttcagcca gtcatgtggt tcggcggcgt aggtgtaggg aaagaagccg cgaccgaagg
     6301 tgacggattc gtcggtgatg acggcctgct cgggcaggag ggcggcgagg gtggcggcga
     6361 cggattccgg ggtgagcttg ccgcgcgcgg gctcgggacg tgggccggga tcggggatgg
     6421 ccgcgcttgg ggcgccgagt tcgtctgcca aacgggccaa cgcttcggcg gcgtcctgat
     6481 cagggcgggc caggacgtgg atatcggcat cggtcgggat cgggctggtg ggcttgttcg
     6541 ggtaggcgaa gaaggtcact ggcttgcgca gcccgcatag gatgacgtgg cgcgtgccgg
     6601 ccagtgcctt cacggcggtg tccaccacgt aggggatgcg gtccaccgga acgcggcccc
     6661 ggccacgcga gatgcgggcg ttggcgccct gggcgatcag gcgcgcgccg gtggcggcgg
     6721 cgatacggtg ggcggattcc agggcgtctg cgcggacggc ctcgccgccg agcacgatga
     6781 ggctgggaag gccgcggcgc aggatctccg cggcgttgcg gacctgcgca ggatcggact
     6841 gtggcctggg gggcaccgag agggcctctg cggcgacgcc gccttcgtcc caggaggtgt
     6901 cggagggcag gatcagcgtg gcgatctggc cgggatgggt gcgggcggcc tgcacggcga
     6961 ccgcggcatc ggcgccgacg gtggcggatg atgtggccgt gcgcacccag gcagaagcgc
     7021 ctcgagccca gccctcggtg tcggcagtca ggggggcgtc gttggggcga tggtaggtgg
     7081 cctggtcacc ggcgatgttg acgatgggtg tctgcgcacg gcgggcgttg tgcaggttgg
     7141 cgaggccgtt ggcgaggccc ggaccgcagt ggagcagcgt tgcggcaggc ttgccggaca
     7201 tacgggcgta gccgtcggcg gctcctgtca ccacgccctc gaacaagccg agcacgcagc
     7261 gcatgccggg cacgcggtcc agcgcggcga cgaagtgcat ttcggatgtg ccggggttgg
     7321 agaagcaggt gtcgacgccg ctgcgcagca gtgtgtggac gaggctttcg gcgccgttca
     7381 tggggcttgt cctttgggaa tcttggggct gggcgaactt gcgcgagagg ggggctgctg
     7441 gcaactgtct cgcgtctgtt gagcgaggga acgtcgatgc cggtcaccgt gctttattcg
     7501 gagaatgtct atggcgacga cagcgtggag cgcgcggtgt tcgggccgga cgtgcgggtg
     7561 ttcctgcctg ggccgacaaa gtctctggcc gacctgccgg atgagctgtg cgcgcaggca
     7621 gatgggttga tgatcctgcg gttccgcgtg tcagaggggg acctggcgcg gttcaccagg
     7681 ctgcgagcgg tgtgccggat gggcgtcggc tacgatggca tcgacaggaa ggcagctgcc
     7741 gagcggcagg tgatggtgct gaacgtgccg gactatggca cgacggaggt ggcggatcat
     7801 gcgatgtcgc tggcactggc gctgcgacgc ggactgctga tccaccatga tgcgcagcgg
     7861 cgcgacccgc cggcggcgtg gtcgtatgtg cgcgatcccg tggtgaagcg gatgggcgtg
     7921 cagacgttcg gcatcctcgg gctggggcgt atcggcaccg cggtggcgtt gcgggcgaag
     7981 gcgttcggct gccgggtggc gttctacgat ccgtacctgc cgaacggggc ggaactggtg
     8041 ttggcgtcgg gcgggcccga acgctggagg aactgctgcg gcagaccgat acgctgagcg
     8101 tgcatacgcc gctgacaccg gagacgcgcg ggatcctggg cgccgccgag atggcaatgc
     8161 tgccgaaggg cgcagtggtg gtgaacacgg cgcgcgggcc gatcgtcgat atcgacgcgc
     8221 tggcggcagc tctgaagagc gggcacctgg cgggcgtcgg gatcgacgtg gtgccggtgg
     8281 agccgccggt cgatccggta ccagaactga tccgggcgta tcgcgcgcgc gagccgtgga
     8341 cggaggggcg gctgatcatc acgccgcact ccgcgttctt ctcgtcgcag gcatgggagg
     8401 atacgcggcg gctggcggcg gagacgatga aggcggcgct gctggggcca cggccgcaga
     8461 acgtgatcgc gcccgaaggg tggtgacgct tcggctcctc gctgatgatc tgactggcgc
     8521 gctggacagt gcggcgcggt tcgtgccgct gacagggccg cttcccatcg tgtggcatga
     8581 tgccgacgtg ccgggcagcg ccgctatcga cagcgggacg cgcgatctgg ccgaggcggc
     8641 ggcgggcacg cgcatcgcgc agttcgcgcc gttgctgcaa ggcgcggata tcgcgttcaa
     8701 gaagatcgac tcgctgctgc gtgggcatgt cgcgctggaa ctcgccgcgt gtatgcgctt
     8761 cttcgactac tgcgtgctgg ccccggcgtt cccgttccag ggccgcatca cccgtggcgg
     8821 gcgccaactt ttccacgctg gcgacacctg gcacgacacc ggtgtcacgc tgccggatat
     8881 gcgcgatgcc gaatccgatg cggatctgga cgccatcgtc gcggacggac atgcgctgac
     8941 cggtcgcgtg ttgtggtgcg gcaccggcgg ccttgctggc gcgctcgcaa atcatcggcc
     9001 ggtgccgcga ctttctctgc caacacccat tctcgctctc atcggcagcg accatccggt
     9061 cagccgcgcg catgtcgccg cggtgccggc ggatcggcgg gccgccgtcg tcgcttgcga
     9121 tctcccgccc aacactgatc ggctcgctgc ccgtgagcac atccgggcca cttttgccgc
     9181 gaccctccgc cagactgaac gtcccggcac gctgttcgtc accggggggg aaaccttgcg
     9241 cgatctgtgc agcgagctcg gcgcgacgca cctggaagtg gatggagagc ttgaaccggg
     9301 cgtgccgact tcgatcctgc gcggcggttc atggaacggg cagcgcattg tctccaaatc
     9361 cggcgcgttc ggcgatgcgg ggttcctggc gcgcctgctg ctatagtgtg ccggaagaca
     9421 tacggaggat gccatgcgac tgggcgttat gctgccgctg accgatatcg gcggcgaacc
     9481 gaacgcgctg cgcgacttcg cgctggcggc ggaggagttg ggctacacca atctcggact
     9541 gcccgatcat gtgctgggcg ccaatgctgc gacccatccc gaggtcgctg gtcgcaacac
     9601 ctcggctgac atgttccagg atccgttcgt cgcgttcggc ttcctcgccg ggctgtgcag
     9661 gccgacgacg gaattctcca cgcaggtgct gatcctggcg cagcggcaga cggtgctggt
     9721 ggcgaagcag gcggcgtgtc tcgatctgct gtgcggcggg cgcttccggt tgggcgtcgg
     9781 agtaggctgg aacccggttg agttcgtggg acttaatgaa agcttcacca accgtggccg
     9841 gcgttccgcc gagcaggtgg tggtgatgca gaagctgtgg gccgagccgc acgtcgtgtt
     9901 caagggcaag tggcacacga tcgacgatgc cggcatcaac ccgctgccgg tgaaccgcca
     9961 catcccggtg tggttcggcg gcaatgtgga ccagacgctg gagcgcatcg ccaccatcgg
    10021 cgacggctgg atcatgaacg cctatgggcc gggcgacgcg gcgctcgcgg aattttcgaa
    10081 gttgcgccgc ctcaccgaag ccgcgggtcg cgatccggcg aagatcggca tcgaggtgtg
    10141 gacctccgtg ggtgccggca gcgaggcgga ctggcgcgaa g
//
LOCUS       DATGEM010000420        19719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1602637, whole genome shotgun sequence.
ACCESSION   DATGEM010000420 DATGEM010000000
VERSION     DATGEM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 19719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19719
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602637"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VKQ27_13410"
     CDS             <1..847
                     /locus_tag="VKQ27_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY89974.1"
                     /translation="TVGFVEGKTSARFSPHPTEWMTLIPTIYNADTLGIRPDLVGRPI
                     TNWKDLVDPAFKGKAAILSIPGIGIMDAAMVMESLGRIKYGDKGNMTKPEIDKTIAFL
                     IETKKAGQFRAFWKSFDESVNLMSSGEVIIQSMWSPAVAAVRSKGIPCKYQPLAEGYR
                     AWGGGLGLAKHLSGLELDAAYEYINWYLSGWVGAYLNRQGYYSAVLETAQKFMTPDEW
                     AYWMEGKPAKADILSPEGKVMEKAGTVRDGGSFTDRMGHVACWNSVMTEDRYMVQKWN
                     EFIVA"
     gene            844..1695
                     /locus_tag="VKQ27_13415"
     CDS             844..1695
                     /locus_tag="VKQ27_13415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89975.1"
                     /translation="MRLRDGWALLQALPLAAVLAVFLLVPMITLIVVSFFDYDSVQII
                     PGFVLTNYQDVLFSATTWWTYLDTLTFTVMVWAITLFVGFWVAYFLTFVVQSRAWQMT
                     WFLVCTVPFLTSNIIRMISWVPFLGRNGLLNSALLRIGVIHAPLEFLLFSNFAVVLAF
                     VHLYTLFMVAPIFNTMVRIDRRLIEAATDAGCSAWRVIVEVIVPLCKPGIAIGSIFVV
                     TLVMGDFITVRLMSGGQSASVGLMISNEVSLLQYPAACANAVVLLAVVLLLVAAIVRV
                     VDVRKEL"
     gene            1696..2502
                     /locus_tag="VKQ27_13420"
     CDS             1696..2502
                     /locus_tag="VKQ27_13420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89976.1"
                     /translation="MPGRYGRLALCVFFGVFVLFLYGPTLTIMILSFQGPDGGLTFPM
                     NGVSLRWFVRLFEQQAVGDIGGALQRSVMLGLMVMAATVVISVAAGMAFRKRFAGAGV
                     LFTLTVASLIVPSILVSLGVGLLFSLAGWDPDWYSSAFGAQLTWTLPFGLLIMFAVLN
                     RFDRRWEEAARDLGASPWQVFRHVVLPIVAPGIVGIALFGFTLSYDEFARTLLTAGSD
                     NTLPLEIFGMTTNITTPVLYALGSLTTYLSAGVIVVALAVMGVLRRRTAG"
     gene            2600..3580
                     /locus_tag="VKQ27_13425"
     CDS             2600..3580
                     /locus_tag="VKQ27_13425"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HLY89977.1"
                     /translation="MKRLPVAVLAVAAIGVLIWLALQRLEATAPASTLYGNVEIRQVD
                     LSFNAEGTVATMPKHEGDRVEQGEVIAALDPATYQSAHDLAVARRDAAKAQLDVLLAG
                     TRPEDIDEARANLAAAQASLANAEASFERQKSLAATNATTKQLLDDARMALDSGRAKV
                     DQMQVLLTRALNGPRPEEIDAARAQLRAAEATVDLSRTQLERTKLVAPANGTIMTRVI
                     EPGTVVLPAANIYSMALDNEVWVRAFAPEPLLPRVVPGSEVTLTTDGTHKTYRGRIGY
                     VSPAAEFTPKTVETPELRTQLVYRLRIRVENADDGIRQGMPMTITLPAAQ"
     gene            3577..5310
                     /locus_tag="VKQ27_13430"
     CDS             3577..5310
                     /locus_tag="VKQ27_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY89978.1"
                     /translation="MTLAVIEDLRHIFPGPHVALAGVSAQIQPGMITGLAGPDGAGKT
                     TLLRLIAGLLRPTSGRVTVFGHDMATDAAAAHPGIGYMPQRFGLYEDLSVAENLDLFA
                     DLHATPRREREQRVARLLRFTGLASFTARLAGQLSGGMKQKLGLACALLSRPRLLLLD
                     EPSVGVDPASRRELWAIVSAMLEEGRDEGMAAVWATAYLDEAERCGHVMLLHEGRLLA
                     QGPPADFLAPMRGRVFRLAVPSGARRVAARRAASDPAVLDALVAGDALRIVLRDGAAP
                     AIAGETLEPAAPRFEDAFVARLAPSVPQGAGERDKPISPSARVRESGGGGASITVADL
                     VRRFGTFTAVDHVSFSVERGEIFGLLGPNGAGKSTIFRMLCGLLRPSEGQARVAGADL
                     LRAPAEARSRIGYMAQRFSLYAELSVTENLRFFARVYGLDRARQTRAIAAALSDFDLI
                     DAGTSIAGELPLGLKQRLALAAALLHGPDILFLDEPTSGVDPLTRREFWARIGTLADA
                     GVTILVTSHFMDEAEYCDRLGIVNQGRLIAVDTPAALRARVRTPELPDPTLEDAFIAL
                     VHATRSPDQVR"
     gene            5348..6475
                     /locus_tag="VKQ27_13435"
     CDS             5348..6475
                     /locus_tag="VKQ27_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89979.1"
                     /translation="MIAALLRMRGLIRKEIKHVLRDPSAILIAFLLPVVLLITNGFGI
                     SLDAREVKLAVVIMAPEETVRGMLQELNASPYLSVQRAFSVHEAEQAVTEGRVRGALI
                     VRDDYSQNLSRTAQWPAQAQLVVDATDPNTARLLEGYVSGALQVWLEGQAREKRQRPV
                     GGIDLQYRDWFNPELRSADFIVPGIIAMVMTMAGTLLTAGSVSREWEHGTMESMLASP
                     ARMSELILARLVCNFALGIGSMIMSVFIATVVFDVPFRGGIVALSAAVSLFLLFALGL
                     GLFISTLARNQFVAGQLSFLTTMMPAMMLSGMLFDIAAMPRWLQFVTYFVPARYLVSI
                     LQTLFLAGDVWPVILPNLAGLAAAAVVTLAATLLVTRRRLD"
     gene            6537..7637
                     /locus_tag="VKQ27_13440"
     CDS             6537..7637
                     /locus_tag="VKQ27_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY89980.1"
                     /translation="MWRRVFALIMKELVGLWKDPKTRFVILVPPLVQVVLFAYAATYD
                     VTNVPIGIWNEDAGAQSAALVRRFAASPAFDVVASFQSPVEARDAIDAKQVAAVLHVP
                     QDFSADVLADRTAQAQFLLDARRSNSALMAQSYAASIVGTYARDLRPRQSPPLIMLTR
                     DWFNPTLESTWFILPGLVGILSMIMALLISSLSLARERELGTLEQLLVTPLRPAEILV
                     GKALPGIIVGMIDANLVIAAALLWFWLPFRGDALVLEATLVLYTLAGVGIGLVLSSFA
                     RTQQQAMLGVFVFASPMIVLSGYAAPVENMPPLAQLISCADPIRYMLVIARGLFLQDM
                     PLGVVLSQAWPMAVIAAATLTAAGFAVRRALS"
     gene            7753..8565
                     /locus_tag="VKQ27_13445"
     CDS             7753..8565
                     /locus_tag="VKQ27_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HLY89981.1"
                     /translation="MNDVTTLEAPPLGGKAIAELAEQLNQLLRLRTFPIGMKLFEDLE
                     AMANVPGLRRPPKGRTFSTCQLVTQSRMAGFTLGITTENIPSFSSCSSVIGLDQVGEV
                     YTSGRKMEGVWFENREAAAAHQAGMSRVPAGRYHGLVVSPLRTARLDPPDVCLFYANP
                     AQMILFINGLQWRSYKRYDFSITGESACADSWGHALKTREVSLSIPCYAERRYGGVAD
                     DEMLMACPPEDLARAVTGLQGLAKAGLRYPIMPFGPQAEPGEGMAKSYAGKS"
     gene            complement(8575..9354)
                     /locus_tag="VKQ27_13450"
     CDS             complement(8575..9354)
                     /locus_tag="VKQ27_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY89982.1"
                     /translation="MARAAAVWDPGQYMRFAGERVRPALDLMAQVRLESADRVVDLGC
                     GAGNVTAILRERFPGADVLGVDGSASMLEKARATVPGCRFEQGDFFQWEPSEPVDLVY
                     SNAALQWVDQHETLFPRLLSFVKPGGAFAVQMPAMHDTPLRTAPYELAKTAPWAEHLR
                     GVSSAPGILSAEAYWDLMRPRVASLDIWQTTYLHALSGDRAVMEWASGSSLRAFLDRL
                     PVELQQPFRQAYSEAVQPDYPRRADGTTLLPFQRLFIVATL"
     gene            complement(9356..9541)
                     /locus_tag="VKQ27_13455"
     CDS             complement(9356..9541)
                     /locus_tag="VKQ27_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY89983.1"
                     /translation="MSLVQWLVLAVTCVIGVAGLLLAASGGENSYALGLMLFVGAVIY
                     VFIQIKQHFDRVDGQHG"
     gene            complement(9568..11025)
                     /locus_tag="VKQ27_13460"
     CDS             complement(9568..11025)
                     /locus_tag="VKQ27_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLY89984.1"
                     /translation="MPELRDEPQHVRPRQSLSGRLWLLTTLAVLLTEVVVFLPLLAHE
                     RRNWLDNHLDDAAIAIVAAVGNTIDQAKASELLRLSGNHAIRMTSPGGSTLTVGDVSR
                     QPVAVIDLEHEDLFMRIRRSLRALIIQENRFVSVRARSPFNPQSEEQIFLSEAALSDA
                     LRQFAGDFAVLALLIAGVTGGLVYLAVLLLLVRPMRRITRSIAAFRADPERTVPLDAD
                     AVTVLSDDEVSVASRELAAMQHELRAALWRNARLAALGTVVAKVSHDLRGILTPALLT
                     SERLQLNADPKVQRAGETLAQAVDRATDLVRRTLDYAREGPPPLALEPVELAPLVDEA
                     ADTARPLNGAFRLNNEVDLAILVHADRTQLFRVLANLLRNAVAAGARTIRVSALHESP
                     TLSVDICDDGPGLPEAVCAELFRPFAGSGAKRGGTGLGLAIARDLMVAHGGDIILVET
                     GDSGTTFRLTLRIATPAAPAEQRTARIAPSAAADV"
     gene            complement(11052..12464)
                     /locus_tag="VKQ27_13465"
     CDS             complement(11052..12464)
                     /locus_tag="VKQ27_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY89985.1"
                     /translation="MADFDLAVIGAGAAGLSVTAAATQLGLRVALIERDRMGGDCLNF
                     GCVPSKALLAASHAATAARSAAHFGVRLLEPQIDWPAVQQHVHGVINSIAPTDSEARF
                     AALGATVLRDEARFVASDALSVGGRRITAGRIIIAAGGSAGVPPVPGLERVPFLTNAT
                     LFDLAEQPGHLLILGGGPIGLEMADAFSGLGSRVTVIEAATIATKEDPELVAGLRGAL
                     AARGIRILEGVTVSAAEPGPALLLSDGRRIEGTHLLIATGRQPNLQALDLAAGNVRAS
                     EAGIVTDRGLRSVSNRRVYAVGDIAAPEGIGSRAFTHIGSYHAGIVVRRALFRLPARL
                     DYASLPRVTYTRPELAQAGLTEAEARAGGRRATLLRWPLADNDRAATERDTTGLVKLV
                     IEGNRVIGAGILAPNAGEMIGLWVLAIAQRVKASALAGMIIPYPTRSEAAKRAAATFF
                     LPKLFAPRTKSLLRLLTRLP"
     gene            complement(12457..13185)
                     /locus_tag="VKQ27_13470"
     CDS             complement(12457..13185)
                     /locus_tag="VKQ27_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HLY89986.1"
                     /translation="MSATPKQSRLLAILRRFWPPLLLIAALVAAWLGGIGQWLSWGTL
                     ARHQMALATWVQAHPLFAPSLYVLVYIVVVALSLPEAAVVTVAGGLLFGTLLGGMLAV
                     VGSSIGAMMLFVAIRHRLAPAIASRGGRLIERLRAEIQHNGFSYMLAIRLVPAFPFWL
                     VNLAAGLSGMRLLPFTAATAIGIIPATFVYASIGAGVGKVLAEGGVPDLSLIFSPGIL
                     GPLIGAAALALLPVAWRRWRRRHG"
     gene            13492..13626
                     /gene="rpmH"
                     /locus_tag="VKQ27_13475"
     CDS             13492..13626
                     /gene="rpmH"
                     /locus_tag="VKQ27_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HLY89987.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRKVLANRRAKGRKRLSA
                     "
     gene            13640..13978
                     /gene="rnpA"
                     /locus_tag="VKQ27_13480"
     CDS             13640..13978
                     /gene="rnpA"
                     /locus_tag="VKQ27_13480"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLY89988.1"
                     /translation="MAASGRLTTRAEFQRAAAKGQKAPMPGVVLQALPRDDGKPARLG
                     FTVTKKIGNAVIRNRTRRRLKEAARLVLAEHPVSGADLVLIGRAGTAGRDFSALQDDV
                     RRALAKVGLA"
     gene            13975..14217
                     /gene="yidD"
                     /locus_tag="VKQ27_13485"
     CDS             13975..14217
                     /gene="yidD"
                     /locus_tag="VKQ27_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLY89989.1"
                     /translation="MTPAAHMAVGVVRAYQWTIRPVIGANCRFLPSCSDYAIEAFRTH
                     GAVRGSGLAARRILRCNPWCEGGYDPVPRCDCEAKQ"
     gene            14217..15980
                     /gene="yidC"
                     /locus_tag="VKQ27_13490"
     CDS             14217..15980
                     /gene="yidC"
                     /locus_tag="VKQ27_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HLY89990.1"
                     /translation="MDQQRLLIAIAISVAILLGFQLLVAPHLPQPPKPEPAQVASTNA
                     PSSNASTGNAQSPLTPPQGSPVSNVPAVPKNVPRVKIAAQDLSGSISLLGARLDDLVL
                     TKYRETLAPDSPNVRLLEPLSDPEPYYVQYGWSAAPGAQVKLPDNDTTWASSGDTLST
                     GHPITLSWDNGEGLTFQIAFSVDDDYMFTVQQSVRNTGGTPVTLYPWSRVRRDYTPET
                     SGYYILFEGMLGIVDNTLQEMTYSSAKSETDKKKSDIAYTAGSSGGWAGFTDKYWLTA
                     LIPAQSVPATVNFTHIKENGDHYQVDYLAKQPETIAPHGDALLESHLFAGAKIVSLLQ
                     SYQSRLQIPRFDYAVDWGYFWFLTQPIFTAIDWLNGVLGNFGLAIMAFTLGVKLLFFP
                     LANYSYRSMSKMRLLGPRMQQMRERFKDEPQRMQQEMMAMYRAEKVNPASGCLPMVVQ
                     IPVFFSLYKVIFVTIEMRQAPFFGWIHDLSQVDPTNVFNLFGLIPFDPSTISPFLHMG
                     AWPLIMGLTMFLQQRLNPPPPDPVQARMFQFMPLIFTFMMARFPAGLIIYWSWNNLLS
                     IAQQWVIMRRTHLSRPGLART"
     gene            15926..16609
                     /gene="yihA"
                     /locus_tag="VKQ27_13495"
     CDS             15926..16609
                     /gene="yihA"
                     /locus_tag="VKQ27_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLY89991.1"
                     /translation="MGDHAAHAPVTSGVGTNLTDEQAALIEAGRKLFASECRFVHAAQ
                     RLDQLPPATGPEVAFAGRSNVGKSSLLNALTRRLALARVSQAPGRTRQLNFFDLSGRL
                     MLVDMPGYGYARASRSVKEDWQGLMFDYLRGRPTLRRVLLLLDARIESKPADIAVMEL
                     LDRAAVTFQLVLTKADGVKPDDLARKLAAVAALARQHPAAHPEVMVTSSETGQGIEDL
                     RAEIATLAG"
     gene            complement(16622..16813)
                     /locus_tag="VKQ27_13500"
     CDS             complement(16622..16813)
                     /locus_tag="VKQ27_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HLY89992.1"
                     /translation="MMDEDDAPVRKPARLQKLVLDTLGIVELREYIDELKAEITRVEA
                     DIERKQGHRSAADAFFKRP"
     gene            16948..17526
                     /locus_tag="VKQ27_13505"
     CDS             16948..17526
                     /locus_tag="VKQ27_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HLY89993.1"
                     /translation="MTLPLMPKATAVWLIEKTALTFTQIAEFCGMHPLEVQAIADGEV
                     AQGIVGYDPIANGQITAEEIRKGEADPDFRLKLAATALPVPKRLRGSRYTPVAKRNDR
                     PDGIAFLLRNYPQLTEAQVSKLLGTTKDTIQKVRERSHWNSANIKPRDPVILGLCSQT
                     DLNAAVAMANDRLSREGRAIPPPPVPEADEVM"
     gene            complement(17545..17718)
                     /locus_tag="VKQ27_13510"
     CDS             complement(17545..17718)
                     /locus_tag="VKQ27_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010669153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HLY89994.1"
                     /translation="MNLQARLDALKERHAALEMRILDEDQRPRPDSDALMRLKLEKLR
                     VKEEMERLRTDNE"
     gene            17844..18047
                     /locus_tag="VKQ27_13515"
     CDS             17844..18047
                     /locus_tag="VKQ27_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HLY89995.1"
                     /translation="MLTDRDSLLRKLHELRSEHRDLDTVIARLAEQGVGDQLHLQRLK
                     KRKLMLKDEIAWLESRLIPDNIA"
     gene            18047..18535
                     /gene="purE"
                     /locus_tag="VKQ27_13520"
     CDS             18047..18535
                     /gene="purE"
                     /locus_tag="VKQ27_13520"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HLY89996.1"
                     /translation="MQPLVGIIMGSQSDWETLRHAAELLDRAGVPHETRIVSAHRTPD
                     RLRDYATEARGRGLKVIIAGAGGAAHLPGMCAAWTPLPVLGVPVESHSLKGMDSLLSI
                     VQMPAGIPVGTLAIGRAGAVNAALLAIAILAIGDAALAAKLDALREAQTAAVADSPTN
                     PA"
     gene            18532..19683
                     /locus_tag="VKQ27_13525"
     CDS             18532..19683
                     /locus_tag="VKQ27_13525"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLY89997.1"
                     /translation="MSRFPLPPNSTIGLVGGGQLGRMSALAAARLGYRCHILTREPGS
                     PATQVSAAVTISDYQEPGGLRAFAAAVDVISFEFENVSAEGLDLLASLKPVRPSPAIL
                     RTSQDRVLEKSFLNGAGVPTAPWASVESFDELREAVERIGLPAVLKTTRLGYDGKGQS
                     RLRRPDDLAAAYAALEPKPLILEGFIDFAQEISVVAARGEDGGYAAFDAVENRHRNHI
                     LDLTLAPARIPEAVDQQAQLIARRVAEALGLVGLLAVEMFVDARGKVLVNEIAPRPHN
                     SGHWTIDACPASQFELHIRSIAGLPLPPAVRHSDAVMKNLVGPEETALWPAILKTPGL
                     IPHLYGKVEARPGRKMGHVTRLFPRGSLPGEFGIAAALGPAAPREDPAS"
BASE COUNT         3292 a         6238 c         6718 g         3471 t
ORIGIN      
        1 tacggtggga tttgtggagg ggaagacctc ggcaagattc tcgccgcatc cgaccgagtg
       61 gatgacgctg atccccacca tctacaacgc cgatacgctg gggatacggc ccgatctggt
      121 ggggcggccg atcaccaatt ggaaggacct ggtcgatccg gccttcaagg gaaaagcggc
      181 gatcctgtcg atcccgggca tcggcatcat ggacgcggca atggtgatgg aatcgctggg
      241 caggatcaaa tacggcgaca agggcaacat gacgaagccc gagatcgaca agacgatcgc
      301 cttcctgatc gagacgaaga aggcgggtca gttccgcgcc ttctggaagt cgttcgatga
      361 gagcgtgaac ctgatgtcgt cgggcgaggt gatcatccag tcgatgtggt cgccggcggt
      421 cgcggcggtg cggtcgaagg gcattccgtg caagtatcag cctctagctg aggggtatcg
      481 cgcgtggggc ggcggactgg gtctcgccaa gcacctcagc ggactggagt tggatgcggc
      541 ctacgaatac atcaactggt acctgtcggg ctgggtgggg gcgtatctca atcggcaggg
      601 gtactacagc gcggtgctgg agactgcgca gaagttcatg acaccggacg aatgggccta
      661 ctggatggag ggcaagccgg caaaggctga catcctcagc ccggagggga aggtgatgga
      721 gaaggccggc actgtgcgcg acggtggctc tttcaccgat cggatggggc acgtcgcgtg
      781 ctggaactcg gtgatgaccg aggaccgcta tatggtccag aaatggaacg agttcatcgt
      841 ggcgtgaggc tgcgcgacgg ctgggcactg ctgcaggcgc taccgcttgc tgcggtgctc
      901 gccgtctttc tgctggtccc aatgatcacg ctgatcgtgg tcagcttctt cgattatgac
      961 agtgtgcaga tcattcccgg cttcgtgctg accaattatc aggacgtgct gttctcggcc
     1021 accacgtggt ggacctatct cgatacactg acattcaccg tgatggtgtg ggccataacg
     1081 ttgttcgtcg gattctgggt ggcgtatttc ctgacgttcg tggtgcagag ccgcgcctgg
     1141 cagatgacgt ggttcctggt gtgcacggtg ccattcctga cctccaacat catccgcatg
     1201 atctcctggg tgcccttcct cgggcgcaat gggctgctga attcagcgct gctgcggatc
     1261 ggggtgatcc atgcgccgct ggaattcctg ctgttctcca actttgcggt cgtgctggcg
     1321 tttgtgcatt tgtatacact gttcatggtg gcgccgatat tcaacacgat ggtgaggatc
     1381 gaccggcggc tgatcgaggc ggcgaccgat gccggctgca gcgcctggcg cgtgatcgtc
     1441 gaagtcatcg ttccgctgtg caagccgggg atcgcgatcg gctcgatctt cgtggtgacg
     1501 ctggtgatgg gcgacttcat caccgtgcgg ctgatgagcg ggggacagag cgcctcggtg
     1561 gggctgatga tcagcaatga agtgtcgctg ctgcagtacc cagccgcctg cgccaatgcg
     1621 gtggtgctac tggcggtcgt gctgctgctg gtcgcggcca ttgtccgcgt ggttgacgtg
     1681 cgcaaggagc tgtaggtgcc gggacgatac ggacggctgg ctctgtgcgt gttcttcggc
     1741 gtgttcgtgc tgttcctgta cgggccgacg ctgaccatca tgatcctgtc gttccagggg
     1801 ccggatggcg gcctcacctt cccgatgaac ggcgtctcgc tgcgctggtt cgtgcggctg
     1861 ttcgagcagc aggcggtggg ggatatcggc ggcgcactgc agcggtccgt tatgctcggg
     1921 ctgatggtga tggcggcgac agtggtgatc tcggtcgccg ccgggatggc gttccgcaag
     1981 cggttcgcgg gggcgggcgt gctgttcacg ctcacagtgg cgagcctgat cgtcccgtcc
     2041 atcctggtaa gcctcggcgt gggcctgctg ttcagcctgg caggctggga ccctgattgg
     2101 tacagctcgg cattcggggc gcagctgacc tggaccttgc cgttcgggct gctgatcatg
     2161 ttcgcggtgc tgaaccgctt cgaccggcgg tgggaggagg cagcgcgcga tctgggcgcg
     2221 agcccgtggc aggtgttccg gcatgtggtg ctgccgatcg tggcgccggg catcgtcggg
     2281 atcgcgctgt tcggcttcac actctcgtac gacgagttcg cgcggacgct gctgacagcg
     2341 ggcagcgaca acacgctgcc gctggagatc ttcgggatga ccacgaacat caccacaccc
     2401 gtgctttacg cgctggggtc gttgacgacg tacctgtcgg cgggggtgat cgtggtggcg
     2461 ctggcggtga tgggggtgtt gcggcggcga acggcgggct gatcagccac cccgggaagg
     2521 caagacggga cctcgacgcg ggccggtcgc aatgacctgc atcaaggcac ccaggttccg
     2581 cctcggctaa cgtgcccgca tgaaacgtct ccccgtcgca gttctcgcgg tcgccgctat
     2641 cggcgtgctg atctggctcg ccctgcagcg gctggaagct accgccccgg ccagcaccct
     2701 gtacggcaac gtggaaatcc gccaggtgga cctgtcgttc aacgccgaag gcactgtcgc
     2761 cacgatgccg aagcatgagg gtgacagggt ggagcaaggc gaagtcattg ccgcgcttga
     2821 tcctgcaacc tatcaaagcg cccacgacct cgcagtggca cgccgcgacg cggccaaggc
     2881 gcagctcgac gtgctactgg ccggcacgcg tcccgaggac atcgatgaag cgcgcgccaa
     2941 tctcgccgct gcgcaggcct cgctggccaa cgcggaggca agcttcgaac gccagaagag
     3001 cctcgctgcc accaatgcga cgacaaagca gcttctggac gacgcacgca tggccttgga
     3061 ttccggccgc gcgaaagtgg atcagatgca ggtgctcctc acccgggccc tcaatggccc
     3121 gcgacccgag gagatcgatg ccgccagggc ccagttgcgc gccgccgaag ccacggtcga
     3181 tctgtcgcgc acgcagttgg agcgcacgaa gctggtggcg ccggcgaacg gcacgatcat
     3241 gacgcgcgtc atcgagccag gcactgtcgt gttgcccgcg gccaacatct attcgatggc
     3301 attggacaat gaagtgtggg tgcgtgcctt tgcgcccgag cctctgctgc cgcgcgtcgt
     3361 gcccggctcc gaggtgacgc tgaccaccga cgggacgcac aaaacctatc gcggccgcat
     3421 tggctacgtg tcgccagcgg ccgagttcac acccaagacc gtggaaacgc ccgaactccg
     3481 cacccagctc gtctatcgcc tgcgcatccg ggtggagaat gcggatgacg gcatccgcca
     3541 gggcatgccg atgacgatca cgctgcctgc cgcgcaatga cgctcgccgt tatcgaggac
     3601 ctgcggcaca tctttcccgg accacatgtc gcgctggctg gtgtttcggc gcagatccag
     3661 ccgggcatga tcactggcct ggccgggccg gatggtgccg gcaagacgac actgctgcgg
     3721 ctgatcgcgg ggttgctgcg tcctacgtcg ggccgcgtca ctgtctttgg ccatgacatg
     3781 gcgacggatg cagcggccgc gcatcccgga atcggttaca tgccgcagcg cttcggcctc
     3841 tatgaggacc tgagcgtcgc ggagaacctc gatctcttcg ccgatctgca tgcgacgccg
     3901 cggcgtgagc gtgagcaacg cgttgcgcgg cttctgcggt tcactggcct ggcgtcattc
     3961 actgcgcgtc tggccggaca gctatcgggc ggcatgaaac agaagctggg tttggcctgc
     4021 gcactgttgt cgcggccccg gttgctattg ctggacgagc cgtcagtcgg cgtcgatccg
     4081 gcctcacggc gggagctttg ggcgatcgtg tcggcgatgc tggaggaggg acgcgacgaa
     4141 ggcatggccg cagtctgggc caccgcgtat ctcgacgagg ctgaacgttg tggccacgtc
     4201 atgctgctgc atgagggccg cctgctggca cagggaccgc ccgcagactt ccttgcgccg
     4261 atgcggggcc gggttttccg gctcgcggtt ccctccggcg cgcgtcgcgt ggcagcacgg
     4321 cgggcggcat cggacccggc ggtgctggat gcgctggtgg caggcgatgc attgcggatc
     4381 gtgctgcggg atggggcggc tcctgcgatt gcgggcgaaa cccttgagcc cgccgcgcca
     4441 aggtttgagg acgcatttgt cgcccgactg gcgccatccg tcccgcaagg ggcaggggag
     4501 cgcgacaaac ccatctcccc gtctgctcgc gttagggagt cggggggagg gggcgcatcc
     4561 atcaccgtcg ccgatctcgt tcgccgcttc ggcaccttca ccgccgtcga ccatgtcagc
     4621 ttctccgtcg agcgcggcga gattttcggc cttctcggcc cgaacggcgc cggcaagtcc
     4681 accatcttcc gcatgctgtg tggcttgctc cgcccgtcgg agggacaggc ccgcgtcgcc
     4741 ggtgccgatt tgctgcgggc gcccgccgaa gcccgctcac gcatcggcta tatggcgcag
     4801 cgcttctcgc tctacgccga attgtcggtc accgagaacc ttcgcttctt cgcccgggtc
     4861 tatggcctgg atcgcgcgag gcagacgcgc gccatcgctg cggctctcag cgatttcgac
     4921 ctcatcgatg ccgggacctc gatcgctggc gaactgccgc ttgggctgaa gcagcgcctc
     4981 gcgcttgcag cagcgctgct gcacggcccc gacatcctgt tcctcgacga accgacgtcc
     5041 ggcgtcgatc cgctcacccg ccgcgaattc tgggcacgga tcggcacact ggcggacgca
     5101 ggcgtcacga tcctggtcac cagccatttc atggatgaag ccgagtactg cgaccggctc
     5161 ggcatcgtga accagggcag gctcattgct gtagatacgc ccgcggcatt gcgtgcccgc
     5221 gtccgcacgc cggagttgcc ggacccgacg ttggaagacg cgttcatcgc gctggtccac
     5281 gccacacgat caccggatca agtccggtga tgacggcaag gggtcgagct ttcgtccggg
     5341 atcgcgcatg atcgccgcgc tgttgcgcat gcgcggcctg atccgcaagg agatcaagca
     5401 cgtcctgcgc gatccctcgg cgatcctgat cgccttcctg cttcctgtcg tgctgctgat
     5461 caccaacggc ttcggcatct cgctcgacgc ccgcgaagtg aagctcgcgg tggtcatcat
     5521 ggcacctgag gaaaccgtgc gcggcatgct gcaggaactc aacgcgtcgc cctacctgtc
     5581 agtccagcgc gccttcagtg tgcacgaggc ggagcaggcc gtcaccgagg gacgcgtgcg
     5641 tggtgctctg atcgtgcgcg acgactattc gcagaacctt agccgcaccg cgcaatggcc
     5701 cgcacaggcg cagctcgtgg tcgacgcgac ggatcccaac acggcccgcc tgctggaagg
     5761 ctacgtctct ggggcgttgc aggtctggct cgaggggcag gccagggaga agcgtcagcg
     5821 accagtgggc ggtatcgatt tgcaataccg cgactggttc aaccccgagc tccgctcggc
     5881 cgacttcatc gtccccggca tcattgccat ggtcatgacg atggccggca ccctgctcac
     5941 tgccggcagc gtttcgcgcg agtgggaaca tggcacgatg gagagcatgc ttgcctcgcc
     6001 ggcgcgcatg tcggagctga tcctggcgcg cctggtctgc aatttcgccc tcggcatcgg
     6061 cagcatgatc atgtcggtgt tcatcgcgac cgtggtgttc gacgtgccgt tccgcggcgg
     6121 catcgtcgcg ctgtcggcgg cggtatcatt gttcctgctg ttcgccctgg gcctcggcct
     6181 gttcatctcg acactggccc gcaaccagtt cgtcgccggc cagctttcat tcctcaccac
     6241 gatgatgccg gcgatgatgc tctccggcat gctgttcgat atcgccgcca tgcctcgctg
     6301 gctgcaattc gtcacctatt tcgtgccagc gcgctatctg gtctcgatcc tccagacact
     6361 gttcctggcg ggcgatgtct ggccggtgat cctgccgaac ctggccggcc tcgccgccgc
     6421 cgccgttgtc acgctggccg ccacgctgct ggtcacgcgc cggaggttgg actgatggcc
     6481 tcggcaggtc agcccattgc gcaggaaggc cgggcttatg ctcgggctga cgcataatgt
     6541 ggcgccgcgt ctttgccctg atcatgaagg agctggtcgg gctctggaag gatcccaaga
     6601 cacgcttcgt catcctggtt ccacccctcg tgcaggtggt gctgttcgcc tacgcggcca
     6661 cctacgatgt gaccaacgtg ccgatcggca tctggaatga ggatgctggt gctcagtcgg
     6721 ccgcgctggt gcgtcgcttc gccgcctcgc cggccttcga tgtcgtggca tcgttccaat
     6781 cgcccgtgga ggcgcgtgac gcgattgacg cgaaacaggt cgccgccgtg ctgcacgtgc
     6841 cgcaggactt ctccgccgat gtgctggctg accgcacggc gcaggcgcag ttcctgctcg
     6901 atgcgcgccg ctcaaactcg gcgctaatgg cgcagagcta tgccgccagc attgtcggca
     6961 cttatgcaag ggacctgcgt cctcgccaaa gcccgccgct gatcatgctg acccgcgact
     7021 ggttcaaccc gacgttggag agcacctggt tcatcttgcc gggcctcgtc ggcatcctgt
     7081 cgatgatcat ggcgctgctg atcagctcgc tgtcgctggc acgcgagcga gagcttggga
     7141 cgctggaaca attgctggta acgccgctgc ggcccgcgga gattctcgtg ggcaaggcat
     7201 tgcccgggat cattgtgggc atgatcgatg cgaacctggt catcgcggcc gcactgctct
     7261 ggttctggct gccgtttcgc ggcgacgcgc tggtgctcga ggcaacgctc gtactctata
     7321 ccctggccgg cgtcggcatc gggctggtgc tgtcgtcatt cgcgcgcact cagcagcagg
     7381 cgatgctggg cgtgttcgtg ttcgcctcac cgatgatcgt gctgtcgggc tatgccgcgc
     7441 cggtcgagaa catgccgccg ctcgcccagc tgatcagttg cgccgatccg atccgctaca
     7501 tgctggtgat cgcccgcggg ctgttcctgc aggacatgcc gctcggggtg gtcctgagcc
     7561 aggcctggcc catggcggtg atcgcggcag ctacactgac ggcggcgggc ttcgcggtgc
     7621 ggcgggcgct gagctgatcc cctgtcatta cgaccccgac ggagctgggc ggccatcctg
     7681 gtaaagttgc acaattggcc tgagattgct tcgctgccgc tcgcaatgac gttgggtccg
     7741 gaggatacgg ccatgaacga tgtaacaacg ctcgaagctc caccgctcgg cggcaaggcc
     7801 atcgccgagc tggcggaaca gctcaaccag ctcctgcgcc tgcgcacctt ccccatcggc
     7861 atgaagctgt tcgaggacct cgaggcgatg gccaatgtgc ccggcctgcg gcggccgccg
     7921 aagggtcgca ccttctccac ctgccagctg gtgacacagt cccgcatggc tggcttcacg
     7981 ctgggcatca ccaccgagaa catcccgtcg ttctcttcct gctcgagcgt catcggcctg
     8041 gatcaggtag gcgaggtcta cacctcgggc cgcaagatgg agggcgtctg gttcgagaac
     8101 cgcgaggccg ccgccgcgca tcaggccggc atgtcgcgtg tccccgctgg ccggtatcac
     8161 ggcctggtgg tctcgccgct gcgcaccgcg cggctggacc caccggatgt gtgcctgttc
     8221 tatgccaatc ctgcgcagat gatcctgttc atcaacgggc tgcaatggcg tagctacaag
     8281 cgttatgact tctcgatcac cggcgagagc gcctgtgccg actcatgggg ccacgcgctg
     8341 aagacgcgcg aggtatcgct ttccatcccc tgctatgccg aaaggcgcta tggcggcgtt
     8401 gcggacgacg agatgctgat ggcctgcccg cctgaagact tggcgcgcgc ggtgaccgga
     8461 ctgcagggct tggcgaaggc gggcctgcgc tatccgatca tgccgttcgg accgcaggcg
     8521 gagccgggcg aggggatggc gaagagctac gccgggaaga gctgaacgag ggccctacag
     8581 cgtcgctaca atgaacagcc gctggaacgg caggagtgtc gtgccgtcgg cgcgccgcgg
     8641 ataatccggc tgaaccgctt ccgaataagc ttgtcggaac ggctgttgca gctccaccgg
     8701 cagtcgatcg aggaaagcgc gcaaactgct gcccgacgcc cattccatca ccgcgcggtc
     8761 accggagagc gcgtgcagat acgtcgtctg ccagatatcc agcgacgcaa cgcgcggtcg
     8821 catcaggtcc cagtatgcct ctgctgacag gatgccaggc gccgagctca caccccgcag
     8881 atgctcggcc catggcgcag tcttcgccag ttcgtatggc gcggtccgca gcggggtatc
     8941 gtgcatggcc ggcatctgga ccgcgaatgc gccacctggc ttcacgaagc tcaacagtcg
     9001 cgggaacagc gtttcgtgct ggtccaccca ttgcagcgcc gcgttcgaat acaccagatc
     9061 aaccggctcg ctgggctccc attggaagaa atcgccttgc tcgaagcggc acccaggcac
     9121 cgtggcccgt gccttctcca gcatgctcgc tgaaccatcg acgccgagaa catcagcgcc
     9181 cgggaaccgc tcgcgcagga tcgcggtgac attgccggca ccgcagccca gatccaccac
     9241 cctgtccgcc gattccagcc gtacctgcgc catcaggtcg agtgccggcc tgacgcgctc
     9301 gccggcgaag cgcatatatt gaccagggtc ccagacggcg gcggcgcgcg ccatctcagc
     9361 cgtgctgccc gtccacgcgg tcgaaatgtt gtttgatctg gatgaacacg tagatcactg
     9421 ccccgacaaa cagcatcagg ccaagcgcgt agctgttctc accgcctgag gcggcgagca
     9481 gcagccctgc caccccgatc acacaggtca cagccagtac gagccattgt acgagcgaca
     9541 cgtgcaccct ccagttgcgc cggcgtccta tacgtcggcg gcggctgacg gggcaatccg
     9601 agccgtccgc tgctcggcag gcgctgctgg cgtggcgatg cgcaaggtga gccggaacgt
     9661 ggtcccgctg tcgccggtct ccaccagtat aatgtcgccg ccatgtgcaa ccatcaggtc
     9721 gcgcgcaatc gccaggccca gcccggtacc gccgcgtttg gcaccgctgc cggcgaacgg
     9781 acggaacagc tcggcgcata ccgcctcggg caggccgggg ccatcatcac agatatcaac
     9841 agacagagtg gggctctcgt gcagggcact gacgcggatc gtccgggcgc cagccgcaac
     9901 ggcgttgcgc agcaaattgg ccagcacacg gaacagctgg gtgcggtcgg catgcaccag
     9961 gatcgccaga tcgacctcgt tgttgagccg aaaagcgccg ttgagcggcc gcgcggtatc
    10021 ggcggcctca tccaccagcg gcgcgagttc cacgggctcg agcgcgagtg gtggggggcc
    10081 ttcgcgtgca tagtcgagcg tccgccgcac gaggtccgtc gcacggtcca cggcctgcgc
    10141 cagggtctcg ccggcgcgct gtactttcgg atcggcattc agttgcagac gttcagaggt
    10201 gagcagtgcc ggtgtcagga tgccgcgcag atcatggctc actttggcga cgacggtgcc
    10261 gagcgctgcc aggcgtgcgt tgcgccacag cgcggcacgc aactcatgct gcatcgcggc
    10321 cagctcgcgg cttgccacgg acacttcatc atccgacagg acagtcacgg cgtcggcgtc
    10381 gagtggtacg gtgcgttccg ggtccgcccg gaatgcggcg atgctgcgcg tgatgcggcg
    10441 catcgggcgg accagcagga gcagcacggc caggtagacc aagcccccgg tcacgcccgc
    10501 gatcaagagc gcgagaacgg caaaatcgcc ggcgaactgg cgcagtgcgt cgctgagcgc
    10561 cgcctcgctc aggaatatct gttcctcact ttgcgggttg aaggggctcc gggcgcgcac
    10621 actgacgaat ctgttctcct ggatgatcag ggcgcgcaac gagcgcctga tccgcatgaa
    10681 gaggtcctcg tgctccaggt cgatcaccgc aaccggctgc cggctgacat cgccaacggt
    10741 caaggtcgag ccgcccggtg aggtcatgcg aatcgcgtgg ttgccggaca gccgcagcag
    10801 ttcgctggct ttggcctggt cgatcgtgtt gccgacggcg gcaacgatgg cgatcgcggc
    10861 gtcatccaga tggttgtcca accagtttcg ccgctcatgg gcgagcaacg gcaggaagac
    10921 gacaacctcg gtcagcaaga ccgccagagt agtgagcagc cagagacgac ccgacaggct
    10981 ctggcggggc cgtacgtgtt gcggttcgtc gcgcaattcc ggcatggcgg gaaattgggt
    11041 cgaaatcgcg gctagggcaa ccgggtcagc aaccgaagca atgacttggt ccggggggcg
    11101 aacaacttcg gtaggaaaaa cgtcgcagcg gcccgtttgg cggcctcgga acgggtgggg
    11161 taggggatga tcatgcctgc cagagcgcta gccttgacac gctgggctat ggccaacacc
    11221 cagagcccga tcatctcgcc agcattgggc gccaggatcc cggcgccgat gacgcgattc
    11281 ccctcgatga cgagcttcac caggccagtc gtatcgcgtt cggtcgccgc ccggtcattg
    11341 tccgcgaggg gccagcggag gagggtggcg cgacggccgc cggcacgcgc ctcggcctcg
    11401 gtgaggccag cctgggcgag ttcgggccgg gtgtaggtga cccgcggcag cgaggcgtag
    11461 tcgaggcggg ccggcaggcg gaacagcgcg cggcggacca cgatgccggc atgataggag
    11521 ccgatatggg tgaaggcacg tgagccgatg ccttccggtg cggcgatgtc gcccaccgcg
    11581 tagacgcggc ggttgctgac gctgcgcagc ccacgatccg tgacgatgcc ggcttcactg
    11641 gcccggacat tgcctgcagc gaggtcgagc gcctgaagat tgggctggcg cccggtggcg
    11701 atcagcagat gcgtcccctc gatacggcgt ccatctgaga gcagcagtgc ggggcctggt
    11761 tctgccgcac tgacggtgac gccctccagg atgcgaatgc cgcgcgcggc cagggcgcca
    11821 cgcagacctg caacaagttc gggatcttcc ttggtggcaa tggtggccgc ctcgatcacg
    11881 gtcacccggg acccgagccc gctgaaggcg tcagccatct cgaggccgat ggggccgccg
    11941 ccaaggatca gcaggtgccc gggctgctct gcgaggtcga acagggtggc gttggtcagg
    12001 aatggaacgc gttccaggcc aggaaccggc ggcaccccgg cgctgccacc ggcggcgatg
    12061 atgatgcgcc cggccgtgat gcggcgcccg ccgacgctga gcgcatcgga ggcgacgaag
    12121 cgcgcttcat ctcgcaggac ggttgcgccg agggcagcga aacgggcctc ggagtcggtc
    12181 ggcgcgatgc tgttgatgac gccatggacg tgctgctgca cggcaggcca gtcgatctga
    12241 ggctcgagca ggcgaacgcc gaagtgtgcg gcgctgcggg cggcggtcgc ggcatgcgac
    12301 gcggcgagca gcgctttgct cggaacgcag ccgaagttca ggcagtcgcc ccccatgcgg
    12361 tcccgctcga tgagggcgac acgcagcccg agttgggtcg ccgccgccgt caccgacagg
    12421 cccgcggccc cggcaccgat cacggcgagg tcgaagtcag ccatgcctgc gcctccagcg
    12481 gcgccaggcg acgggcaaca aggccagtgc cgcggccccg atcaggggtc ccagaatgcc
    12541 cggggaaaag atcaacgaca gatccggcac accgccctcc gccagcacct ttccgacgcc
    12601 ggcaccgatg gaggcataga cgaatgtggc cgggatgatc ccgatcgccg tcgcagcggt
    12661 gaagggcaac aggcgcatgc cgctcagccc ggcggcaagg ttcacgagcc agaaagggaa
    12721 tgcggggacg agccggatcg ccagcatgta ggagaagccg ttgtgctgta tttcggcgcg
    12781 cagacgttcg atcaggcgtc cgccacgcga ggcgattgca ggcgccaggc ggtgacggat
    12841 cgcgacaaac agcatcatcg ctccgatcga agaaccgacg accgcaagca tgccgccgag
    12901 cagcgttccg aacagcaggc ctccggcgac cgttacgacc gcggcctcag gcagcgagag
    12961 ggcgacgacg acgatataaa cgagcacgta aagcgacggg gcaaacaagg gatgggcctg
    13021 gacccaggtt gcgagcgcca tctggtgccg cgcgagagtg ccccaactca gccactgccc
    13081 gatgccgccc agccatgcgg ccaccagcgc ggcgatcagg agcaggggcg gccagaaccg
    13141 gcgcaggatc gctagcaggc gagattgctt tggcgttgcg ctcatcgtcc cgtggcaatc
    13201 cggcgttgtt gggcgcacat gaaaatgcca gggggcggac cctctggcag gttcgcgttg
    13261 tcggtcgggt gcttatcagg cgaggaccgt acggaagtcc agcgccttcc cctggcctgc
    13321 ggtctgcgcg gcaaaagtgg agtgaggcgc ccttcgacca atcttccggg agcggcctcg
    13381 ccgtgattga cgcccccgaa ccgcccccct ataagccggc gcctgcgcgg gcagagtgcc
    13441 tgccggcgcg ccgcgtctcc gcggtcatcg ggcagcatcg gagtatcgac tgtgaagcgc
    13501 acctatcaac cgtccaagct ggtccgcaag cggcggcatg gcttccgcgc ccggatggcc
    13561 acggttggtg gccgcaaggt gctggcgaac cgcagggcga agggccgcaa gcggctctcg
    13621 gcctgaaccg agtgcgcgca tggccgcgtc tggccgtctg acgacacggg cggagttcca
    13681 gcgcgcggcg gccaaagggc agaaggcgcc gatgccaggc gtggtgttgc aggcattgcc
    13741 gcgcgacgac gggaagcccg cccggcttgg tttcactgtg acaaagaaga tcggcaacgc
    13801 tgtgatccgc aaccgcaccc gccgccgcct gaaggaagcc gcccggctgg ttctggccga
    13861 acatccggtc tccggcgcgg atctggtgct gatcggacgt gccggcacgg cgggcaggga
    13921 tttctccgcg ttgcaggacg atgtgcggcg cgcccttgcc aaggttggcc tggcatgacg
    13981 ccggccgcgc acatggcagt cggcgtcgtg cgcgcctacc agtggacgat ccgcccggtg
    14041 atcggggcga attgccgctt cctgccaagc tgcagcgact atgcaatcga ggcgttccgg
    14101 acccacggtg ctgtgcgcgg cagcggtctg gcggcgcggc gcatcctgcg ctgtaaccca
    14161 tggtgcgagg gcggctacga tccggtccct cggtgcgact gcgaggcgaa gcaataatgg
    14221 accagcaacg gcttctcatc gcgatcgcga tctcagtcgc catcctgctc ggcttccagt
    14281 tactggtggc gccgcatttg ccccagccgc ccaagccgga gccggcccag gtggccagca
    14341 ccaatgcgcc atccagcaac gcgtcaaccg gcaatgcgca gtcgccgttg acgccgcctc
    14401 agggctcgcc ggtcagcaac gtgccggcgg tgccgaagaa cgtgccccgg gtgaagatcg
    14461 cggcgcagga cctgagcggc tcgatcagcc tgcttggcgc acggctcgat gatctcgttc
    14521 tgaccaaata tcgcgagaca ctggcgccag actcgccgaa tgtgcggctg ctcgagccgc
    14581 tgtcggatcc cgagccatac tacgtgcagt acggctggag cgcggcgccg ggcgcgcagg
    14641 tgaagctgcc ggacaacgac acgacctggg cctcgtccgg cgacacgctg agcacgggtc
    14701 acccgataac gttgtcatgg gacaacggcg aaggcctgac attccagatc gcgttctcgg
    14761 tcgacgacga ctacatgttc acggtccagc agtcggtgcg gaataccggc ggcacaccgg
    14821 tcacgctgta tccctggtcg cgtgtgcggc gtgactacac gccggagaca tcaggctact
    14881 acatcttgtt cgagggcatg ctcggcatcg tggacaacac gttgcaggag atgacctact
    14941 ccagcgccaa gagcgagacc gataagaaga aatccgacat cgcctacact gctggttcgt
    15001 cgggtggctg ggcaggtttc accgacaagt actggttgac cgcgctgatc cctgcgcaat
    15061 cggtgccggc cacggtgaac ttcacccaca tcaaggagaa cggtgaccat taccaggtgg
    15121 attacctggc caagcagccc gagacgatcg cgccgcacgg tgatgcattg ctggaatcgc
    15181 acctgttcgc gggtgcgaag atcgtctcgc tgctgcagag ctatcaatcg cggctgcaga
    15241 tcccgcgatt cgattatgcg gtggactggg gctatttctg gttcctgacg caaccgatat
    15301 tcactgccat cgattggctc aacggggtgc tgggcaactt cgggcttgcc atcatggcgt
    15361 tcacgctcgg cgtgaagctg ctgttcttcc cgctggcgaa ttattcctac cgctcgatga
    15421 gcaagatgcg gctgctcggt ccgcggatgc agcagatgcg cgagcggttc aaggatgagc
    15481 cgcagcgtat gcagcaggaa atgatggcga tgtaccgggc ggagaaggtg aacccggcat
    15541 caggctgcct gccgatggtc gtgcagatcc cggtgttctt ctcgctctac aaggtgatct
    15601 tcgtcacgat cgagatgcgg caggcaccgt tcttcggctg gatccatgac ctgtcacagg
    15661 tcgaccccac caacgtgttc aacctgttcg ggctgatccc gttcgatccg agcacgatct
    15721 cgccgttcct gcacatgggc gcctggccgc tgatcatggg cctgacgatg ttcctccagc
    15781 agaggctgaa cccgccgccg cccgatccgg tgcaggcgcg catgttccag ttcatgccgc
    15841 tgatcttcac gttcatgatg gcgcgtttcc cggcgggact gatcatctac tggagctgga
    15901 acaatctgct ctccatcgca cagcaatggg tgatcatgcg gcgcacgcac ctgtcacgtc
    15961 cggggttggc acgaacctga ccgacgaaca ggctgcgctg atcgaggcag ggcgcaagct
    16021 gttcgcctcg gaatgccgct tcgtgcatgc ggcgcaacgg ctggaccagt tgcccccggc
    16081 caccgggccc gaggtggcgt tcgctggccg gtccaatgtc ggcaagtcgt cactgctcaa
    16141 tgcgctgaca aggcgtcttg ccctggcacg ggtgtcgcag gcgcctgggc ggacgcggca
    16201 gctcaacttc ttcgatctca gtggccggct gatgctggtg gacatgcccg gctacggcta
    16261 tgcacgcgcc tcaaggtcgg tgaaggaaga ctggcagggc ctgatgttcg actacctgcg
    16321 cgggcggccg accctgcggc gcgtgctgct gctgctggat gcgcggatcg agagcaagcc
    16381 cgcggacatc gcggtcatgg agttgctgga ccgcgcggcg gtgacgttcc agttggtgct
    16441 gaccaaggct gatggcgtga agccggacga cttggcgcgg aagcttgcgg cggttgcggc
    16501 gttggcgcgc cagcacccgg ccgcgcaccc ggaggtgatg gtgacgagca gcgagaccgg
    16561 gcaggggatc gaggacctgc gcgctgaaat tgccacactg gcgggctgaa acccgcggcg
    16621 atcagggccg cttgaagaag gcgtcggcgg cggaacggtg gccctgcttg cgctcgatat
    16681 cggcctcgac ccgcgtgatc tccgccttca attcgtcgat atattcacgg agttccacaa
    16741 tccccagtgt atccaggacc agcttctgca gacgcgccgg cttgcggact ggggcgtcat
    16801 cctcatccat catgctgcac ctctcgtgtc gttgatccca gcataaaccg tgccggaact
    16861 cttgacccac ccctgaccgg ttgtcatatc ccgcccccgg cctgcccgca ccttgacggc
    16921 gcgccgtggc ccgaggatga gcatgccatg accctgccgc tgatgcccaa agccaccgcc
    16981 gtgtggctga tcgagaagac cgcgctcacg ttcacacaga tcgctgagtt ctgcggcatg
    17041 cacccgctgg aggttcaggc gatcgcggac ggtgaggtgg cgcagggtat cgtcggctac
    17101 gacccgatcg cgaatgggca gatcacggcc gaggaaatcc gcaaggggga ggccgatccg
    17161 gacttccggc tgaaactggc ggcgaccgcg ctgccggttc cgaagcggct gcggggttcg
    17221 cgttatacgc ctgtggccaa gcggaacgac cggccggacg gtatcgcgtt cctgctgcgc
    17281 aattatccgc agctgaccga ggcgcaggtg tcgaagctgc tggggacgac caaggacacg
    17341 attcagaagg tgcgggagcg gtcgcactgg aacagcgcca acatcaagcc tcgcgatccg
    17401 gttatcctcg ggctgtgcag ccagaccgac ctgaacgctg cggttgccat ggccaatgac
    17461 cggctgtcgc gcgagggacg cgcaataccg cccccgccgg tccccgaggc cgacgaggtc
    17521 atgtagccgg tcagaccggc ctgatcactc gttatcggtg cggaggcgct ccatctcctc
    17581 cttgacgcgc agcttctcga gcttcaatcg catgagcgca tcgctgtctg ggcggggtcg
    17641 ctgatcctca tcaagtatgc gcatttccag cgcggcatgg cgttccttca gtgcatcgag
    17701 acgggcctgc aggttcatcg accttcctcc gctccaagac agccacgata aatatggtaa
    17761 tccgtttgac acgaacgagg ccatgacaaa aacgccatcc agggactcgg tcggcgcgag
    17821 gggttcatgc tattgaaggg gtcatgctga cggatcggga ctcgctgctg cgcaagctgc
    17881 acgaactccg cagcgagcat cgtgacctcg atacggtgat cgcgcgcctg gctgagcagg
    17941 gggtggggga ccagttgcat ctgcaacggt tgaagaagcg caagctgatg ttgaaggacg
    18001 agatcgcctg gctggagagt cggttgatcc cggataacat cgcctgatgc agccccttgt
    18061 cggcatcatc atgggcagtc aatcggactg ggagacgctg cggcacgctg cggaactgct
    18121 ggaccgggcg ggggtgccgc acgagacgcg gatcgtgtcg gcgcatcgca cgccggaccg
    18181 gcttcgcgac tacgcgaccg aggctcgggg ccgagggctg aaagtcatta tcgcgggcgc
    18241 aggtggggcg gcgcatttgc ctggaatgtg tgctgcgtgg accccgctgc cggtgctggg
    18301 agtgcctgtg gaatcccata gtctcaaggg gatggacagt cttctttcga tcgtccagat
    18361 gccggcgggg attccggtcg ggacgctcgc catcgggcgg gccggggcgg tgaacgctgc
    18421 gctgctggcg attgcaattc ttgcgatcgg cgacgctgcg ctggcggcaa agctggatgc
    18481 gctgcgcgag gcgcagaccg cggcagtggc ggacagtccg accaatcctg catgagccgc
    18541 ttcccgctgc cgcccaactc gaccattggc ctggtgggtg gcgggcagct cggtcgcatg
    18601 tcggcgttgg ccgcggctcg gctgggctac cggtgccata tcctgacccg cgagccgggc
    18661 agcccggcaa cccaggtctc ggcggcggtg acgataagcg actaccagga acccggaggg
    18721 ctgcgggcgt ttgcggcggc agtggatgtc atcagctttg agttcgagaa tgtcagcgcg
    18781 gaaggccttg atcttcttgc atcactgaag cctgtccgcc cgtcgccagc gatcttgcgg
    18841 acaagccagg accgggtgct ggagaagtcc tttttgaacg gtgccggcgt gccgacggct
    18901 ccctgggcat cggtggagag tttcgacgaa ctgcgcgagg cggttgagcg gatcggactg
    18961 ccggcggtgc tgaagacgac gcggctgggt tacgacggca agggccagtc gaggctgcgg
    19021 cggccagacg atctggcggc ggcgtatgcg gcattggagc cgaagccgtt gatcctggag
    19081 ggtttcatcg acttcgcaca ggagatcagc gtggtcgcgg cgcgcggcga ggacggcggc
    19141 tatgccgcgt tcgatgcggt ggagaaccgg catcggaacc acatcctgga cctgacactg
    19201 gcgccggctc gcattccgga ggcggtggac cagcaggcgc agttgatcgc gcgccgcgtg
    19261 gccgaggcgc tggggttggt cggtctgctg gccgtcgaga tgttcgttga tgcgcgcggc
    19321 aaggtgctgg tgaacgagat cgcgccacgc ccgcacaact cggggcactg gacgatcgat
    19381 gcctgcccgg cgagccagtt cgagctgcac atccggtcga ttgccgggct gcccctgccg
    19441 ccggcggtgc ggcattcgga tgcggtgatg aagaacctgg tgggtccgga ggaaacggcg
    19501 ctgtggccgg cgatcctgaa gacgccgggg ctgatcccgc atctgtatgg caaggtggag
    19561 gcccggccgg ggcggaagat ggggcacgtg acacggctgt tcccgcgcgg gtcgctgccc
    19621 ggcgagttcg gtatcgcggc ggcgctggga ccggcggcgc cgcgcgaaga tccggcaagc
    19681 tgaatatctg gcgttgcgag ggtggcgccg gcgggagta
//
LOCUS       DATGEM010000421         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1395675, whole genome shotgun sequence.
ACCESSION   DATGEM010000421 DATGEM010000000
VERSION     DATGEM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395675"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1862)
                     /locus_tag="VKQ27_13530"
     CDS             complement(402..1862)
                     /locus_tag="VKQ27_13530"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HLY89998.1"
                     /translation="MDLELGLDPDAARRLSRLPLLTPLRSGKTRSRTIRIIWHDSAEH
                     ALAKNGLVLAEQRPQWRLERLAPDDATWPPGGPVPVVASARSLDELGRALPQPLVPIA
                     AFEGRARSIDLACEHGPVVMTLLAGAIRAVAGEHRISRVHLEGAVQPVQALAVALAGQ
                     LDLSVPRTCLAAEALAIVTGVPPAGRHHGAPDLPAGCSVAEAFAHTVGHLTDVILHYA
                     PAAAAGSDGPEPVHQMRVAVRRLRSAIKVFRRGVQSTEVDAADAGLKALASRLGPTRD
                     WDVFVTETFAAVAGAFPHEKHLKRLLAAAERRRRAAHSELRAFLTSFEFHRLGIELAC
                     LASGKEWQETLGEAEQAELALSLEDFAARVLDRRLKRLIQVESTLAELEPSALHAVRL
                     SAKRLRYAAEIFAPLYPGKSTSRYLRRLGNLQDRLGALNDVSVASTLLTELAGSGSQA
                     FASGLVLGYSGAHGGRTRGKIDAVWQKFRRQAPFWE"
     gene            complement(1867..2388)
                     /locus_tag="VKQ27_13535"
     CDS             complement(1867..2388)
                     /locus_tag="VKQ27_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLY89999.1"
                     /translation="MHQLLLLRHAKSSWDDPKLSDRDRPLNKRGRRAAAAMRQAMQDL
                     GLSPDLILVSTARRTQETLAALEPWDETPLIEPLESLYLASARQLLDVLRGTNETVRS
                     VMLIGHNPGMHELAVSLAGSVESEQTHRLRDGFPTTALAEFAIGGTWESLGTSGGQLV
                     RYLIPRDLPETTG"
     gene            complement(2499..>2714)
                     /locus_tag="VKQ27_13540"
     CDS             complement(2499..>2714)
                     /locus_tag="VKQ27_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90000.1"
                     /translation="IVRQRDRPEPDAYLNGYIDQQRVAIAAKFHSVRAYGRAMPHEAE
                     RNPPVRRYRKPGPRATRISQRGAGRDE"
BASE COUNT          440 a          851 c          955 g          468 t
ORIGIN      
        1 cgcgccagtg aggccgggcg tagagtggaa tgagactcga ccggatcact cacccatcac
       61 tgcggcgagt tgagtcgggc tcggcttact gcctgtggcc ggcgcctcgg ccgacggcga
      121 caaatgggca caagttgtgc cggcggtaag aaagaaaatg gaaccgcaaa atacgcagaa
      181 acacgcagat gaataccgac gagggcgggg tggaccgcct gcccaatcga agcatcggct
      241 gtgtgaatga tcggaggctg cgcaacatcg gtaaccttgc cttgagatca aacgcatcgc
      301 cctggccctg ttgggctccg ccgccctttg cgtgttctgc gtgctttgcg gctccatctt
      361 attactgttc gaacagaccg cggtgcgcgg atgccttcgg tttactccca gaacggcgcc
      421 tgccggcgga atttctgcca gaccgcgtcg atcttgccgc gcgtgcgtcc gccatgggcg
      481 ccggagtagc cgagcacgag acctgaagcg aacgcctggc tgccgctgcc ggccagctct
      541 gtgagcaatg tcgaggcgac ggacacgtcg ttcagcgcgc cgagccggtc ctgcagattg
      601 cccaggcggc gcagatagcg gctggtcgac ttgcccggat agagaggggc gaatatttcc
      661 gcggcgtagc gcaggcgctt tgcgctcagg cggacggcat gcagggccga tggctcgagt
      721 tccgcaagcg tgctttccac ctgaatgaga cgtttcaggc ggcgatcgag tactctggcg
      781 gcaaagtcct cgagcgatag cgccagttct gcctgttctg cttcgccgag cgtttcctgc
      841 cattccttac cgctggccag gcaggccagt tcgatgccca ggcgatggaa ttcgaagctg
      901 gtaaggaatg cacgcaactc gctatgggcg gcgcgacggc ggcgctcggc ggcggcgagc
      961 aggcgcttca gatgtttctc gtgaggaaat gccccggcaa ccgcggcgaa tgtctcggtg
     1021 acgaacacat cccagtcgcg cgttggcccc agccgactgg ccagagcttt caggccggca
     1081 tcggctgcat cgacttccgt ggactgcacg ccgcggcgga acactttgat ggcggagcgc
     1141 aggcgccgca ccgcgacacg catctgatgc acgggctcgg gtccgtcgct gccagcggcg
     1201 gcggccggcg catagtgcag gatcacatcg gtcaggtggc cgacggtgtg ggcgaaggct
     1261 tcagcgacgg aacacccggc gggcaggtcg ggggcgccgt ggtggcgtcc cgccggtgga
     1321 acaccggtga cgattgcgag cgcctcggct gccaggcaag tccgtggcac ggacaggtcg
     1381 agctggccgg ccaatgcgac cgcgagcgcc tgcaccggct gcacggcacc ttccagatgg
     1441 acgcggctta tccggtgttc gcccgcgacg gcgcggatgg cgcccgccag cagcgtcatg
     1501 acgacgggac cgtgctcgca cgcgagatca atgctgcggg cacgtccctc gaacgcggcg
     1561 attggcacca agggttgcgg cagcgcgcgc cccagttcat ccaggctgcg tgccgaggcc
     1621 acgaccggca ccggtcctcc gggcggccag gttgcgtcgt ccggtgccag gcgttccagg
     1681 cgccactgcg ggcgctgttc ggccaggacg aggccgttct tcgcaagcgc atgttccgcg
     1741 ctgtcgtgcc agatgatgcg gatggtccgg ctgcgcgtct tgccgctgcg cagcggtgtc
     1801 agcaatggca gccgcgacaa gcgcctggcg gcgtccggat cgaggcctag ctccaggtcc
     1861 atcgcctcag cctgtggtct ccggcagatc gcgtgggatc aggtagcgca cgagttggcc
     1921 gccactggtg ccgagcgact cccaggtgcc gccgatggcg aactcggcca atgcggtggt
     1981 gggaaagcca tccctcaggc ggtgggtctg ttcggattcc actgagccgg ccagtgatac
     2041 ggcaagttcg tgcatgccgg gattgtggcc gatgagcatg acgctgcgca cggtctcgtt
     2101 ggtgcctcgc agaacgtcga gcaactggcg cgcgctggcg aggtagagcg actcgagcgg
     2161 ctcgatcagc ggcgtttcat cccatggctc cagcgcggcg agcgtttcct gcgtgcggcg
     2221 cgcggtcgag accaggatga ggtcggggga caggccgaga tcctgcatgg cctggcgcat
     2281 agcggcggcg gcccgccgac ctcgcttgtt cagcggccga tcgcggtcgg acagctttgg
     2341 gtcgtcccag gacgacttgg cgtgccgcag cagcaaaagt tgatgcattc cgcgatcctg
     2401 ccacggagcg ggggacttgt cccctccctg aagcaggttc agtgtgaggg cgctgacatg
     2461 gtagttgggg aacctggaca gccatgatct gccggcgatc attcatcgcg gccggcgccg
     2521 cgctggctta tgcgcgtggc gcgagggccg ggcttccggt accgcctgac aggcggattg
     2581 cgttccgcct catgcggcat ggctcgccca taggcacgca cactgtggaa tttcgcggcg
     2641 atggcaacac gctgctggtc gatatagccg ttgaggtacg cgtctggctc gggccgatcc
     2701 cgctggcgca ctat
//
LOCUS       DATGEM010000422         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1498945, whole genome shotgun sequence.
ACCESSION   DATGEM010000422 DATGEM010000000
VERSION     DATGEM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498945"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VKQ27_13545"
     CDS             complement(<1..821)
                     /locus_tag="VKQ27_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY90001.1"
                     /translation="MSPGDQVNILLVDDQPAKLLSYEVILADLQENLIKANSANEALH
                     HLLRHECAVVLIDVVMPDLDGFELAAMIREHPRCQRTAIIFVSAIQMSEPDFLRGYQA
                     GAVDYVPVPVVPELLRAKVRVFADLYRKTRQLEQLNAELERRVEERTAELAQANAELE
                     RRVEERTREREDAWAQVHEMQKLESLGQLTGGVAHDFNNLLMVILGNLQLLRKRLPDD
                     PRLMRLVDGAVQGADRGATLTKRMLAFARRQELRPEPVDVFRLVQGMEEMLRRTLG"
     gene            complement(832..>2567)
                     /locus_tag="VKQ27_13550"
     CDS             complement(832..>2567)
                     /locus_tag="VKQ27_13550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY90002.1"
                     /translation="FTVNVDPTLRGITTDSKRLQQVLKNLLSNAFKFTEHGGVQLSIS
                     AVASGWTPGHLVLDHAPTVVAFEVSDTGIGIQPEKQRIIFEAFQQADASTSRKYGGTG
                     LGLAISRELSNLLGGEIQLRSKPGVGSTFTLYLPLSYIGPATPSREAAAYGGASARAE
                     VALPNQVRLADRPNETVADDRLTIEDGDNVLLIVEDDTHYARILVDLAHDHGLKVLVA
                     ARGTDALTLAREYQPTAVSLDVFLPDMLGWTVLSQLKQNPATRHIPVQIVTLDEDRQH
                     GLARGAFSFMTKPTTTEGLQEALGKITDYVRPRRKKLLVVEDNAAERLSITELLGHDD
                     IDIVTVETGAEAIEALRDMPADCVVLDLRLPDMSGFEVLERIRDDDDLANVPVVVFTG
                     RELSPEEDAQLHTMARSVVVKGVESPERLLDETALFLHRVVSDLPAEKQRMLEMLHSS
                     DESLVGQKVLLVDDDARNIFALSSVLERRGMEVLTATTGSEAIETLQSTPGLAIVLMD
                     IMMPEMDGYQTMEVIRAQPAFRRLPIIALTAKAMKGDREKCLEAGASDYLAKPVNTEQ
                     LLSTLRIWLHR"
BASE COUNT          453 a          802 c          810 g          502 t
ORIGIN      
        1 ccgagcgtgc ggcgcagcat ctcctccatg ccctgcacca ggcgaaatac gtccaccggt
       61 tccggccgaa gttcctggcg gcgggcgaag gccagcatgc gcttggtcag cgtcgcaccg
      121 cgatccgcgc cttggaccgc accatcgacc agacgcatca gtcgtgggtc atccggcagg
      181 cgcttccgca gcaattgcag gttgcccagg atgaccatca gcagattgtt gaagtcgtgc
      241 gcgacgccgc cggtaagctg accgaggctt tccagcttct gcatctcatg gacctgggcc
      301 catgcatctt cgcgttcgcg agtccgttcc tcgacgcggc gttccagctc ggcgttcgcc
      361 tgcgccagtt cggccgtgcg ttcttctacg cgccgctcca gctcggcgtt gagctgctcg
      421 agctgccgcg tcttgcggta tagatcagcg aatacacgca ccttggcgcg aagcagttcc
      481 ggcacgacag gcacaggcac gtaatcgacc gcacctgcct ggtaaccgcg caggaaatcc
      541 ggctcactca tctggatcgc cgagacgaag atgatcgcgg tgcgctggca gcggggatgc
      601 tctcggatca tcgccgcgag ttcgaagccg tcgagatccg gcattacgac gtcgatcaga
      661 acgacggcgc actcgtgccg gagcaagtga tgcaacgctt cgttcgccga gttggctttg
      721 atgaggttct cctggaggtc cgccaggatc acctcatagc tcagcagctt ggccggctgg
      781 tcgtcgacca gcaggatatt gacttggtcg cccggactca tggcagccgc ctcagcgatg
      841 cagccagatc cgcagcgtgg agagtagctg ctccgtgttc accggcttgg ccagatagtc
      901 ggatgcacca gcttccaggc atttctcacg gtcacctttc atcgccttgg cggtgagcgc
      961 gatgatcggc aatcgacgga atgccggctg ggcgcggatc acttccatgg tctgatagcc
     1021 atccatttcg ggcatcatga tatccatcag cacgatcgca aggcctggcg tggattgcag
     1081 cgtctcgatc gcctcgctgc cagtggttgc agtcagcact tccatgccgc ggcgctcgag
     1141 cactgagctc aacgcgaaga tgttgcgcgc gtcgtcgtcc accagcagca ctttctgtcc
     1201 gacaaggctt tcgtcggagc tgtgcaacat ctcgagcatg cgctgcttct ctgccggcag
     1261 atcggagacg acgcgatgca ggaacagggc agtctcatcc agaaggcgct ccggtgattc
     1321 gacgcccttc accacgacgc tgcgagccat tgtatgaagc tgcgcatcct cctccggtga
     1381 cagctcgcgt cctgtgaaca cgactactgg tacattggcc aggtcatcat cgtcgcggat
     1441 gcgctccagt acctcgaaac cggacatgtc gggcaggcgc aaatcgagaa cgacgcaatc
     1501 ggccggcata tcccgtaacg cctcgatcgc ttcggcgccg gtttccaccg tgacgatgtc
     1561 gatgtcgtcg tggccgagca gttcggtgat ggacaggcgc tcggcggcgt tgtcctcaac
     1621 aaccagcagc ttcttgcgcc gcggcctgac gtaatcggtg atcttcccga gcgcctcctg
     1681 cagaccttcc gtggtggtgg gcttggtcat gaaggagaac gcaccgcgcg ccaggccgtg
     1741 ctggcgatcc tcgtccaacg tgacgatctg cacggggata tggcgtgttg caggattttg
     1801 cttgagctgg ctcagcacag tccagccgag catgtcggga aggaacacat cgagcgacac
     1861 ggcggtgggc tgatactcgc gtgccagcgt cagggcatcg gtgccgcggg cggcgaccag
     1921 caccttcagc ccgtgatcat gcgccagatc gaccaggata cgggcatagt gcgtatcgtc
     1981 ttccacgata agcagcacgt tgtcgccatc ctcgattgtg aggcgatcgt ccgccactgt
     2041 ctcgttcggg cgatcggcga ggcgcacctg gttgggcagg gcgacttcgg cgcgcgcgga
     2101 ggcgcctccg tacgcggccg cctcccggct cggcgtggcc ggtccgatat acgagagcgg
     2161 caggtaaagg gtgaaagtgc tcccgacgcc aggcttgctg cgcaactgga tctcgccgcc
     2221 gagcaggttg gagagctccc gactgatggc gaggccaagg cccgtgccac catacttgcg
     2281 gcttgtggac gcgtcggcct gctggaacgc ctcgaaaata atgcgctgct tctcaggctg
     2341 aatgccgatg ccggtgtcgc tgacctcgaa tgccacgacg gttggcgcat gatccaggac
     2401 gagatggcca ggcgtccagc cacttgcgac ggctgagatg ctgagctgta cgccgccatg
     2461 ttcggtgaac ttgaacgcgt tcgagagcag gttcttcagc acctgttgca ggcgcttgct
     2521 gtcagtggtg atgccgcgca gcgttggatc gacgttcacg gtgaagg
//
LOCUS       DATGEM010000423         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1291496, whole genome shotgun sequence.
ACCESSION   DATGEM010000423 DATGEM010000000
VERSION     DATGEM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291496"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3075)
                     /gene="gltB"
                     /locus_tag="VKQ27_13555"
     CDS             complement(<1..3075)
                     /gene="gltB"
                     /locus_tag="VKQ27_13555"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HLY90003.1"
                     /translation="MAADHGVFVGDTMAREEPGLSGHSTTRDGADARTGDWGLYRSEY
                     ERDSCGFGLIASLDDQASHWVVETAISSLNRLTHRGAIATDGKTGDGCGLLIKMPTRF
                     LREAAVEAGFSLTPRFAAGLVFLDPDPVKGAEARRVLTAEIEREKLQLAGFRPLPTDP
                     AACGLEALKTLPAIVQVFVNAGSDIDESTLNRKLYMVRRRAEKVLESSDRMFYIPSLS
                     ASTIVYKGMVMPQFLAQFYPDLKDPRMEASVVVFHQRFSTNTLPQWRLAHPYRYLAHN
                     GEINTVQGNRSWAVARGPLFRSPVLPDLADVLPVVSLTGSDSQSLDNMLELLLVGGLD
                     PMHALRMLIPPAWQSVDMIDPDLKAFYEYYATHMEPWDGPAGIVVTDGRYACCTLDRN
                     GLRPARWVITKNRHITIASEAGVWDYLPEDVVRKGKLGPGEILALDLQTAELMDTRQV
                     DDLLKSRHPYKVWLKKGVRYLQTDLIDPRLAAEPFDRDTLALFQKMYNVSAEERDEII
                     RVLAEDESEAVGSMGDDTPMPVLSKKVRSLYDFFRQQFAQVTNPPIDSLRESIVMSLQ
                     TQIGPEANIFVPAQQHAEQIVLSSPILSQRKLRQIMGLEDSGVPHEFIDLQYDEKLDL
                     KAAILTVCEQAEAAVRAGKLVLLLSDRYLVKGKIPIQALLATGAVHHRLVKTGLRCKC
                     NILVETGTARDSHHFACLIGYGATAVYPYLAYQTLFDLMRKGAVKMDADTRQELGRSY
                     RRGIRKGLFKIMSKMGISTIASYRSAQLFEIVGLGEDVVSLCFEGSDSRIQGADFVDL
                     KADAEYLAKRAWDPTETVEQGGLLKYMHNGEYHMYNPDVIAMLQSAVMSGDYEQYKQF
                     AALVNERPVSCIRDLLKLKPDAPAIPLDQVESIASILKRFDSAGMSLGALSPEAHEAL
                     AIAMNRLGARSNSGEGGEDPARYGTEKNSKIKQVASGRFGVTPEYLINAEVLQIKVAQ
                     GAKPGEGGQLPGHKVNDMIARLRFARPGVGLISPPPHHDIYSIEDLAQLIFDL"
BASE COUNT          595 a         1033 c         1044 g          629 t
ORIGIN      
        1 gagatcgaag atcagttgcg ccagatcctc gatggaatag atgtcgtggt gagggggcgg
       61 cgaaatgagc cccactccgg ggcgcgcgaa acgcaggcgc gcgatcatgt cgttgacctt
      121 gtggcccggc agctggccgc cttcgccggg cttcgcaccc tgcgccacct tgatttgcag
      181 cacctcggcg ttgatgaggt attccggcgt caccccgaag cggcccgagg cgacctgttt
      241 gatcttggag ttcttctcgg ttccgtagcg cgccggatct tcgccgcctt caccggagtt
      301 cgaacgtgca cccagacgat tcatggcgat ggcgagggct tcatgggcct ccggcgacaa
      361 ggcgcccaag gacatgccgg cactgtcgaa gcgcttcaag atcgacgcga ttgattccac
      421 ctggtcgaga ggaatggccg gcgcatccgg cttcaatttg aggagatcgc ggatgcacga
      481 caccggccgc tcgttcacca aggcggcgaa ctgcttgtac tgttcgtaat cgcccgacat
      541 caccgccgac tgcagcatcg cgatgacatc ggggttgtac atgtggtact cgccgttgtg
      601 catgtatttg agcaggccgc cctgctccac ggtctccgtc gggtcccagg cgcgcttcgc
      661 caggtattcc gcatcggcct tcaagtcgac aaagtccgcg ccttgaatcc ggctgtccga
      721 gccctcgaag cacagcgaca ccacgtcctc gcccaggccc acgatctcga agagctgcgc
      781 gctgcggtag ctcgcgatgg tggagatccc catcttggac atgatcttga acaggccctt
      841 gcgaatgccg cggcggtagc tgcggccgag ctcctggcgc gtgtccgcgt ccattttcac
      901 cgcgcccttg cgcatgagat cgaacagcgt ctggtaggcc agatacggat agaccgccgt
      961 tgcaccgtag ccgatcaggc aggcgaaatg atgggagtcg cgcgccgtgc cggtctcgac
     1021 caggatgttg cacttgcatc gcagccccgt cttgaccagg cgatgatgca ccgcgccggt
     1081 ggcgagcaac gcctggatcg gaatcttgcc cttgacgagg tagcggtccg acagcagcag
     1141 caccagcttg ccggcgcgca ccgccgcttc ggcctgctcg cacactgtga ggatggcggc
     1201 cttgagatcc agcttttcgt cgtactgcag gtcgatgaat tcgtgcggga cgccggaatc
     1261 ctcgaggccc atgatctggc gcaattttcg ctgcgacaga atgggtgaac tcaacacgat
     1321 ttgctcggcg tgctgctgtg ccggcacgaa gatgttggct tccgggccga tctgcgtctg
     1381 cagcgacatg acgatgctct cgcgcagcga gtcgatcggc gggttcgtca cctgggcgaa
     1441 ctgctggcgg aagaaatcgt agagagagcg caccttcttc gacagcaccg gcatcggcgt
     1501 gtcgtcgccc atcgagccga ccgcctcgct ctcgtcctcg gcgagcacgc ggatgatttc
     1561 atcacgctcc tcggccgaca cgttgtacat cttttggaac agcgccaagg tgtcccggtc
     1621 gaagggctcc gccgccagac gcggatcgat gagatccgtc tggagatagc gcaccccctt
     1681 cttcaaccac accttgtagg gatgccggct cttcagcaga tcgtcgacct gcctggtgtc
     1741 catcagctcg gccgtttgca gatcgagggc gaggatctcg ccgggcccca acttgccctt
     1801 gcgcaccacg tcctcgggaa gatagtccca aaccccggcc tcggaggcga tggtgatatg
     1861 gcggttcttg gtgatgaccc agcgcgcagg gcgcaatccg ttccggtcca gcgtgcagca
     1921 tgcataccgg ccgtcggtca ccacgatgcc ggcgggaccg tcccacggct ccatgtgggt
     1981 ggcgtagtac tcgtagaaag ccttgaggtc ggggtcgatc atgtcgaccg actgccatgc
     2041 gggcggtatc agcatgcgca gcgcatgcat gggatcgagt ccgcccacga gcagcaattc
     2101 aagcatgttg tcgagggatt gcgaatcgga gccggtcaac gacaccacgg gcagcacgtc
     2161 ggccagatcg ggcagcaccg gcgaacggaa cagggggccg cgcgccacgg cccagctgcg
     2221 attgccctgt accgtgttga tctcaccgtt gtgtgcgaga taacggtagg gatgcgccaa
     2281 ccgccactgc ggcagcgtgt tggtggaaaa tcgctggtgg aacaccacga ccgacgcctc
     2341 catgcgcgga tccttgagat ccggatagaa ctgcgccagg aattgcggca ttaccatgcc
     2401 cttgtagacg atggtgctcg ccgacagcga cggaatgtag aacatccggt cgctcgattc
     2461 gagcaccttc tcggcgcgcc gccgcaccat gtagagcttg cggttcaacg tggactcgtc
     2521 gatgtcggaa cccgcgttca cgaacacctg cacgatggcg ggcagcgttt tgagggcctc
     2581 caggccgcac gccgcgggat cggtgggaag cggccgaaat ccggccagct gcagcttttc
     2641 ccgctcgatc tcggcggtca gcacgcggcg ggcttccgcc cctttgaccg gatccggatc
     2701 gagaaagacc agtcccgcgg cgaaccgggg ggtcagtgag aagccggcct cgaccgcggc
     2761 ttcccggaga aagcgcgtcg gcatcttgat caggaggccg cacccgtcgc cggttttgcc
     2821 atcggtggcg atggcgccgc gatgcgtcag gcgattgaga gaactgatgg ctgtttcgac
     2881 cacccaatgg ctggcttggt catcgaggct ggcgatgagt ccgaagccgc aggaatctcg
     2941 ttcatactcg gagcgataca gtccccaatc tccggtgcgc gcatccgcgc cgtcgcgcgt
     3001 ggtcgagtga ccggatagtc ctggttcttc ccttgccata gtgtcgccca caaatacgcc
     3061 gtgatccgcg gccattccgc agaactacaa taaaaacaga aacttaactc ggttctaggc
     3121 gctcaatgga tgcgcgccgg gtggggcaat cgccgctggc cgtgcctagg atatgggatc
     3181 catgatacct aggtcgtcaa cgtcgtcaac cggctggcgg cgtcaaccgg cgatgtagcg
     3241 ttccagctgc tcgatcttct ggctttggtc ctcgatgacg gccttgacga ggtcgccgat
     3301 g
//
LOCUS       DATGEM010000424         2922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_32942, whole genome shotgun sequence.
ACCESSION   DATGEM010000424 DATGEM010000000
VERSION     DATGEM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32942"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VKQ27_13560"
     CDS             complement(<1..465)
                     /locus_tag="VKQ27_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HLY90004.1"
                     /translation="MSKHDFTPAEFADRLYRLRQGIAAAGVDWLLVLHPVSIRWLIGQ
                     DTKSYTVFQCLAVSAEPRPLVMFTRGTERAEFEADTMAEEFRFYGGGEPEDPMQAFAR
                     FADELGLRKARVGMEVPGGYLQPPHYVALKALLGEALVQDGSGLVTALRAVKS"
     gene            complement(476..1270)
                     /locus_tag="VKQ27_13565"
     CDS             complement(476..1270)
                     /locus_tag="VKQ27_13565"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90005.1"
                     /translation="MGVILSAHRVTKSFAGTGEPLVAIRDLSLDVEEGEFLCLLGASG
                     CGKSTMLSLFAGFTAPTAGQIMLRGRPITGIEPRCGMVFQSYALFPWKTVRGNVEFGL
                     KMQGVARGVRRERAQRFIDMVKLTGFEERYPAELSGGMQQRVTLARILAADPEVLLMD
                     EPFAALDAMTRQIMQEELLGIHEASGKTTVFITHSIDEALILADRIVVMSARPGRIKA
                     ILHNRLPHPRHVSVQLSQDYAALKAEVWGLVESELLAHDEQSRSRH"
     gene            complement(1274..2092)
                     /locus_tag="VKQ27_13570"
     CDS             complement(1274..2092)
                     /locus_tag="VKQ27_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90006.1"
                     /translation="MSDAVIAAPAPSPSGRRLRFRHVLLLINVAAVATVFLLWWAATR
                     FGWIAPIFLPSPGDVWHAARLTMRNGDLLTDVAVSARRVFLGFGLAALIAVPLGVVMG
                     VWQPAKAIMDPFVSLLRPLPSITWIPLTILWLGIGEAQKIAIVFMGSWIYILLYTVES
                     TRRVDPLLVRAARNLGASDLAVMKEVILPGSLPGIIAGLKVTLAISWSCVLTAEMIAA
                     QSGLGALIWQAKDWGNLALVLVGMLCISLTVLVADWVMVRLERLLLPWERSRRN"
     gene            complement(2094..>2922)
                     /locus_tag="VKQ27_13575"
     CDS             complement(2094..>2922)
                     /locus_tag="VKQ27_13575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90007.1"
                     /translation="FKEQGIDTELKNIPASGDRIAALTAGSVAFSNLGRIAAIVEMAR
                     NNPSFYFFANIDDSPGNEGCWARPGFTSFKSLKGHKVAANTSAQITMNGLLANEGMTE
                     KDIQFVNLPGGEMAGALAKGDVDAACVWQPLLDNVEKGTPDGKLLGLDSDTPNFKQFG
                     TMASPDVMIISRKLVDEQPDLARKLTVAIFKGVEYTNANPKETAETVAHYFRKPPEEV
                     LAAMKTFKYFGATDWQQHMKLHAAQMQFLAQWLYDNGKIPSLPESAKWENTSFVPKP"
BASE COUNT          562 a         1019 c          842 g          499 t
ORIGIN      
        1 cgacttcacc gcccgcaatg cagtgaccag accagagccg tcctgcacca gggcctcgcc
       61 cagcaacgcc ttcaacgcga catagtgcgg cggctgcaga tagccgcccg gcacttccat
      121 ccccacgcgc gccttgcgca atccgagttc atcggcaaag cgcgcaaacg cctgcatcgg
      181 gtcctctggc tcgccaccgc catagaagcg gaattcctcg gccatcgtgt ccgcttcgaa
      241 ctccgcccgt tccgtcccgc gggtgaacat caccagcgga cgcggttcag cggaaaccgc
      301 caggcactgg aataccgtgt agctcttggt gtcctgcccg atcagccagc ggatcgaaac
      361 cggatgcagc accagcagcc agtcaacacc ggccgccgcg atcccttggc gcagtctgta
      421 tagacggtca gcgaactccg ccggggtgaa gtcatgtttc gacatggttt tccgatcagt
      481 gcctggaacg tgattgctcg tcatgcgcca gcagctcgct ttccacgagc ccccagacct
      541 ccgccttcag tgcggcgtag tcctgcgaca gctgcacgga gacatggcgc ggatgcggca
      601 ggcgattatg caggatcgcc ttgatccttc ccggccgcgc cgacatcacc acgatacgat
      661 cggccaggat cagcgcctca tcgatcgaat gcgtgatgaa gaccgtggtc ttgccgctcg
      721 cttcgtggat gcccagcagc tcctcttgca tgatctgccg cgtcatcgca tcgagcgcgg
      781 cgaacggctc atccatcaac agcacctctg ggtcggccgc caggatgcgc gccagcgtca
      841 cccgctgctg catcccgccc gatagttccg ccggatatcg ttcttcgaac cccgtcagct
      901 tcaccatgtc gatgaaccgc tgcgcccgct cgcgccgcac gccgcgcgca acgccttgca
      961 tcttcaggcc gaactcgaca ttgccgcgca ccgtcttcca cgggaacagc gcatacgact
     1021 ggaacaccat gccgcagcga ggctcgatgc cggtgatcgg ccgaccgcgc agcatgatct
     1081 gcccggcagt cggcgccgtg aaaccggcaa acagcgacag catggttgac ttcccgcacc
     1141 ccgaggcacc cagcaggcag aggaactcgc cctcctcgac atcgagcgag agatcgcgga
     1201 tcgccaccag cggctcaccg gtgccggcaa agctcttggt cacgcgatgc gcggacagga
     1261 tcacacccat ggctcagttc cgcctgctgc gctcccaggg caggagcagc cgctccagcc
     1321 gcaccatcac ccagtcggcc accagcaccg tgagcgagat gcacagcatc ccgaccagga
     1381 caagcgccag atttccccag tccttcgcct gccagatcag cgcaccgaga ccgctctgcg
     1441 ccgcgatcat ttccgccgtc agcacgcagg accaggaaat cgccagcgtc accttcagcc
     1501 ccgcgatgat ccccggcagc gagccaggca ggatcacctc cttcatcacc gcaagatccg
     1561 aagccccaag attgcgtgca gcccgcacca gcagcggatc aacccgccgc gtgctctcta
     1621 ccgtgtatag caggatgtat atccacgatc ccatgaacac gatcgcgatc ttctgcgcct
     1681 cgccgatgcc aagccacagg atggtcagcg gaatccaggt gatcgacggc agcggccgca
     1741 gcagcgacac gaacggatcc atgatcgcct tggcaggctg ccagacaccc atcacgacgc
     1801 cgagcggcac ggcgatcaat gcggcgagac cgaatcccag gaacacgcga cgcgccgaga
     1861 ccgcgacatc ggtcagcagg tcgccgttgc gcatggtgag ccgtgcggca tgccagacat
     1921 cgccaggcga gggaaggaag atcggcgcaa tccagccgaa ccgcgtcgcg gcccaccaca
     1981 gcagaaacac cgtggcgaca gccgcaacat tgatcagcaa cagtacatgg cggaaacgca
     2041 gacgccgccc cgatggagac ggggcgggag ctgcaatcac cgcgtcactc atgtcagggc
     2101 ttcggaacga agctggtgtt ctcccatttc gcggattccg gcaaggatgg gatcttgcca
     2161 ttgtcataca gccactgtgc caggaactgc atctgcgccg cgtgcagctt catgtgctgc
     2221 tgccagtccg tcgcgccgaa atacttgaag gttttcatcg ccgcgagcac ctcctccggc
     2281 ggcttgcgga agtagtgcgc caccgtctcg gcggtctcct tcggatttgc gttggtatac
     2341 tcgaccccct tgaatattgc gacggtcagc ttgcgggcga ggtccggctg ttcgtcgacc
     2401 agcttgcgcg aaatgatcat cacgtcaggc gaagccatcg tgccgaactg cttgaagttc
     2461 ggcgtgtcgc tgtccaagcc cagcagcttg ccgtccggcg tgcctttctc gacgttatcg
     2521 agcagcggct gccagacgca cgccgcatcc acgtcaccct tggccagcgc gccggccatc
     2581 tcgccgcccg gcagattgac gaactggatg tccttctcgg tcatgccttc gttcgccagc
     2641 agcccgttca tggttatctg cgcagacgtg ttggctgcga ccttatggcc cttgagcgac
     2701 ttgaagctgg tgaagcccgg ccgcgcccag cagccctcgt tgcctggcga atcatcgata
     2761 ttggcaaaga agtagaagct cgggttgttg cgggccatct ccacaatcgc ggcgatgcgc
     2821 ccgagattgc tgaacgccac cgatccggca gtgagcgcgg cgatccggtc gcccgaggcg
     2881 gggatgttct tgagttcggt gtcgatcccc tgctccttga ag
//
LOCUS       DATGEM010000425        10420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1028473, whole genome shotgun sequence.
ACCESSION   DATGEM010000425 DATGEM010000000
VERSION     DATGEM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028473"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..968)
                     /locus_tag="VKQ27_13580"
     CDS             complement(594..968)
                     /locus_tag="VKQ27_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY90008.1"
                     /translation="MADITGALADIRNTLGRIEKAVELLHTAPRLEPVDPSVKVSTAA
                     AILDCDESFVRKLVKDEELSSHQLGKRGIRIYLSSIEDYRRRSTVLAAGGTPAAGKPK
                     PKPKQSASFNEAMAFMKSRGLV"
     gene            1007..1252
                     /locus_tag="VKQ27_13585"
     CDS             1007..1252
                     /locus_tag="VKQ27_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90009.1"
                     /translation="MALLAGIRIGSLGPSDWRTSEFVTKTVRYRAYLPAARHWIIGWG
                     RPIADRHSERIFILDTLIHAITPICMIVAFCVGATYG"
     gene            complement(1268..1636)
                     /locus_tag="VKQ27_13590"
     CDS             complement(1268..1636)
                     /locus_tag="VKQ27_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY90010.1"
                     /translation="MVTDPEPRPDAWRLTGLPDLYTLKEVAKTLHCSPSWLKGQIAEG
                     KLVAAKVAGTYLVTAEDLRSFFDSCKNRPPSKAPSVDVMPSSLEDRQAMKEAAQKVLK
                     ERRTAALVLAREKRGPAGSV"
     gene            complement(1654..1857)
                     /locus_tag="VKQ27_13595"
     CDS             complement(1654..1857)
                     /locus_tag="VKQ27_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90011.1"
                     /translation="MTIDFGAVLGGLSAVTSICAATLSFRAPAATKGIGLLTVLAGLL
                     ALSAAGWAQLHQPVKPWLYGASG"
     gene            complement(1946..2022)
                     /locus_tag="VKQ27_13600"
     tRNA            complement(1946..2022)
                     /locus_tag="VKQ27_13600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1986..1988),aa:Arg,seq:ccg)
     gene            complement(2186..2755)
                     /locus_tag="VKQ27_13605"
     CDS             complement(2186..2755)
                     /locus_tag="VKQ27_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90012.1"
                     /translation="MTKFEILRGLPSYGPIACPFPEDGRGQFREGLAVKFWKKSGATW
                     VGNFQRGFTSFDQVMNYPDGHLVIVIAGGDGYVIDPDLMKQTHKFGGGLTFAIELVDL
                     NLVLIGDNIRVGTFTVDGPGWDSGRISWDGIRNIVVNGSSLSGEAYTPIDDCWQAFGI
                     DLLTGEVTGAVYPEQMATAVKLRRASAEE"
     gene            2776..3105
                     /locus_tag="VKQ27_13610"
     CDS             2776..3105
                     /locus_tag="VKQ27_13610"
                     /inference="COORDINATES: protein motif:HMM:NF019023.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HLY90013.1"
                     /translation="MRNARLYYRENNLISLARYDILHIGESQDEMMVSANLVERIRAI
                     CCVKGVIDAHTELWGDLGLHGDDAFELLEAIHTDCGVDFSDLDFNRFFPNETEGIYYW
                     WGRRIGL"
     gene            3177..3491
                     /locus_tag="VKQ27_13615"
     CDS             3177..3491
                     /locus_tag="VKQ27_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90014.1"
                     /translation="MKRTSSYAITGWKAVALAPFLIPLGLILTLLGFNKRTIERAPED
                     VAGFIADMIDGSGGEWDWDEFECVPITDPALDAIRLRAAVFGPPNANIAEAEAIAAAR
                     SA"
     gene            3705..4319
                     /locus_tag="VKQ27_13620"
     CDS             3705..4319
                     /locus_tag="VKQ27_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90015.1"
                     /translation="MAARNKLSKSDKSRDDDAAPRVLNRAQHAALVNKFLSVLVRTQL
                     KSGKAAGIPKLLAEEIRAAGSVDRWIKSDGARRTRYEQLKEKLGPRAAALPSGHVDAA
                     LERFLAMIAAIQIRRKQEISIPRPFAKLVLAAGSVENWIEGDEAIRTLYEATYKRVLV
                     SEAERLAKRLPRGAAPPARIEPQLPHLPLSPQGWGKPAGKADEE"
     gene            complement(4573..6402)
                     /locus_tag="VKQ27_13625"
     CDS             complement(4573..6402)
                     /locus_tag="VKQ27_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90016.1"
                     /translation="MASSLSNRYWKVLISLLALLATALLLMELPGALTAIVGQERASL
                     GAIIALSGEYGSSKRIIAQLALDGPLSRAGAAVGDGVTFDHLKDFYHPVAGEEVGLIL
                     HHGSERHRAVVTTEILPWAPDAAFNALGSLIVGPIFCAVGLLIGFRQPGSSAHRALSL
                     CFIGVMLCFSDYLNLRFLPSFVPILATRSLVVAANSMVVYTASRFATLFPDDRPQGIR
                     RVLHRYGLPPLALLCLASTIDEIANIYGFEIPPLHPLSQLQQIAMPLFLIVALSAGRA
                     HSSGDLRTRYNWLLVSLGMATLAALPSTFDWTVAGWKVYSISNTFGLGCAILILAYAV
                     LRHRVIDVGFALNRAAVYGVTSALLLSSFGLLEWTAEHFLHFESREQNILLDAGLALG
                     VFLSFHRLHDFVEHWVERILFHEWHAREAALRKFVRRAAHFTDGERLLDAFVAVLGDF
                     TGGAGYAIYLDARNGGLTARRSGLMAAPEWVSENEREVIALCDMNAPLGGDEISLIPG
                     CALALPMMHRGVLGGFVLLGPKPQGDGYRPDEVDVLDFAVRQIGLDLRALEADALSKQ
                     VSVLSRENEAITAENQRLHDSVGKFMAGMQTGSLAADRTAVLE"
     gene            6785..7930
                     /locus_tag="VKQ27_13630"
     CDS             6785..7930
                     /locus_tag="VKQ27_13630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HLY90017.1"
                     /translation="MVMTAASEGDHRVQHYARFWQAEDPLADAVAAAFAERAAGGGRA
                     MLETALDRGIGAVDDPPPALVALFRQLDEVPYWVDWDRIDRGSAAFLRTGLLGMAVLN
                     LFCLPIMYYSPCGNKPLVFTGNLLKRAPRRLAETGRFVLECSRPDGLRRFSDGFKTNV
                     KVRLMHAQVRRLLRRSGRWNPKWGAPVNQLYMAGTNVALSAGLIEGLERLGYRMGAAD
                     VEALLHLWRYTGYISGIAPELQISTREEGRHLGELIHQLEGQPDDDSRALITALMSSS
                     YHPRLEGATWTQKINYGVSRKLIGNELADDLGYPRSHWGWTVSLARPMAGSLGLIHRY
                     VPLLRSAAPRWGDRTWDFMLDLIMGGSKADYHAPAHLARPAPEEKAA"
     gene            7927..9426
                     /locus_tag="VKQ27_13635"
     CDS             7927..9426
                     /locus_tag="VKQ27_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLY90018.1"
                     /translation="MKSETLPTLSPRDWELLREHVTVIRRPRGDVILAEGGHRRAIFI
                     VRSGAVRVEQSQNGLGIALALLGEGEIFGEMGFVENVPASASVIVQDDAELEVIEGDA
                     LESIKAADPGFSVRFYHSLAISLARRLRLTSARLSRAGAGEAAQVNRHRVARTGNITA
                     RQVPPALGDSLDQFEQTMLSVKLALRAGSLDAEAAVRQLSVSCDGVAALLSDYTEVDP
                     LVEIGYNDLLSFRDQDHLEAGIGDYVFRETFGTLLLSATMARCYAKPRGFPDDHETMA
                     MIAAGQAQGDDRLGPLIDRWFLDRPLCLSRRASTELMRRSLAEVVASRPAGEAIRIAS
                     LASGTAGELLDLCAISDGAAIEASLVDLDGEALQAAARRAERLGLSDRVVLVNGNVVG
                     PPGEVVSLLPQHIAYALGLCDYLTDDEVVILLDRLYELVVPGGCALVTNLSPDNGDRL
                     LMQHLLDWQAYHRSGDQIRDLFARSAFGDRAVEIFTDASGVTVFARCDK"
     gene            9605..9790
                     /locus_tag="VKQ27_13640"
     CDS             9605..9790
                     /locus_tag="VKQ27_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90019.1"
                     /translation="MRLLVLLLILFAIFHVALLIKVAFAIVLAAALTIGLWVLWRLKW
                     VILGILGLEEVLSARRP"
     gene            10038..10403
                     /locus_tag="VKQ27_13645"
     CDS             10038..10403
                     /locus_tag="VKQ27_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HLY90020.1"
                     /translation="MAEKIKVGIATHAELKERALQIARGERRRQPDEPKIWFTSLESL
                     AKILSEANRKLLKIIDEQHPSSLAELELLSGRKASNLSRTLKTMSQCGLIRLVPGKRG
                     SIAPEVLVREVQMELSIVA"
BASE COUNT         1976 a         3133 c         3300 g         2011 t
ORIGIN      
        1 cgcggccatg gcggggctcc aggcagaaag caagatgcat actttatcat actggtgacg
       61 tggtttcacc acgtcaccgc aggcagaatt tcttgaattt ctggccgcga tcggcccgag
      121 gaaattattg agaattactc aatagctcga tccgaaggtg cggacctggt cgaggtgcag
      181 gtggctcaac ccagcacatt cgggccatct atgaggacga attcaaacgg agaagggagg
      241 tcatttcgaa tgctgggcta gtcgatgagg ccaggaagac ggtcgagctt tcatcgacct
      301 taatacctgg ctggcgatgc aatatcgtcc cggcgtcgcc gtggtcaacg gtccccgagg
      361 accggcgatc gtcaatgtct ctcccatacg cgtagagaaa cggcgtcgcc aggttgtgat
      421 tggcgctcgg cgatcgatca tgagcagttc cgtgtctgac ggggggctgt cccaggtgag
      481 cgagagcagt tcacttcgcc gcatgccggt ttctagggct agcagaacgg cagggacgag
      541 ccatggattg gtcgcgcctt cgcaggcctc tagtaaacgg cccgcgtcgt cgcctacacg
      601 aggcctctgc tcttcatgaa ggccatcgcc tcattgaagg aagcggattg cttgggcttt
      661 ggcttgggtt tccccgccgc cggcgtgccg cccgccgcca gcacggtcga cctccgcctg
      721 taatcctcga tcgacgagag atagatccgg atgcccctct ttccgagctg gtgcgatgat
      781 agctcttcgt ccttcaccag cttgcgcacg aagctttcgt cgcagtccag aatcgccgcc
      841 gcggtcgaca ccttcacgga agggtcgacg ggctcgaggc ggggtgcggt gtggagcagc
      901 tcaaccgcct tttcgatgcg gccgagtgtg ttcctgatgt cggcaagagc acctgtgata
      961 tcggccatgg ctgccccctc ttttatcgtc gccgtgcatc gtcgatttgg cgctgctggc
     1021 aggtatccgc atagggtcgc tcggcccgtc tgactggcgc acgtcggagt ttgtaacaaa
     1081 aaccgtgcga tatagggcgt atcttccggc cgcccggcac tggatcattg gttggggccg
     1141 gccgatcgcc gaccggcaca gcgaaaggat attcatcttg gatacgctca tccatgccat
     1201 caccccgatc tgcatgatcg tggcgttctg cgtcggcgcg acttacggct gatcgtgtag
     1261 gccctgctca caccgagccg gcggggcctc tcttctctct ggcgaggacg agcgcggctg
     1321 tccgacgctc tttcaagacc ttttgtgcgg cctctttcat ggcttgcctg tcctcgagtg
     1381 acgatggcat tacgtccaca ctgggggctt tcgacggcgg gcggttcttg cagctgtcga
     1441 aaaatgatcg gagatcttct gctgtgacga gataggttcc cgccaccttc gcggcaacca
     1501 acttcccctc ggcgatttgt cccttcagcc aggacggaga acagtggagg gtcttggcga
     1561 cctccttgag agtgtacagg tccggcagtc cggtcaggcg ccacgcgtcc ggacgcggtt
     1621 cgggatcggt gaccatgcgg gggccctgaa ggcttatccc gaagccccgt aaagccaggg
     1681 tttcacaggc tggtggagtt gtgcccatcc tgctgccgac aaggcgagta ggccggccag
     1741 tacggtcagc aagccgatcc ctttggtggc ggccggcgcg cgaaaggaga gagtggccgc
     1801 acagatcgaa gtgacagccg acagccctcc aagaaccgcc ccgaaatcga tggtcattta
     1861 gacccctccg actgtctgct tgggtgtcga atttgcgaca actgcttacc ccctgcttac
     1921 ccgacgggga cggatcgttg gaaagtggtg cacccggaga gattcgaacc cccggcctct
     1981 gccttcggag ggcagcgctc tatccagctg agctacgggt gcgctggtcg catgtgccga
     2041 tcggcacgtg cgaacggttc gtatagcgca ggtggccaag agatgcaaac gggagatgga
     2101 gcgcgcgcaa tggggaggcg gcagagccgg ggttcgaatc tctccaggcg cacggtcgat
     2161 gtgccgggag cgtggcggag gcctatcatt cttcggcaga agctcgccgt agctttacgg
     2221 ccgttgccat ctgctcagga tagaccgcac cagttacttc gccagtgagc agatcaatcc
     2281 caaacgcttg ccaacaatca tcgattggcg tgtatgcctc gcctgatagc gagcttccat
     2341 tgacgacaat attgcgaatc ccgtcccatg agattctgcc gctatcccag ccgggcccgt
     2401 cgacggtaaa tgtcccgact cggatattat cgcctattaa aacgaggttg aggtccacga
     2461 gctcaatcgc gaatgtgagt ccaccaccga atttatgagt ttgcttcatc aaatcaggat
     2521 cgatgacgta tccatcacct ccggcgatga caatgactag atgtccgtca ggatagttca
     2581 ttacctgatc gaacgaggta aaaccgcgct ggaagttgcc aacccaggtt gcccccgatt
     2641 ttttccagaa cttgactgcc aaaccttcgc gaaattgccc gcggccgtct tcgggaaatg
     2701 ggcatgcgat tggcccatag ctcggcaatc cacgcaggat ctcgaactta gtcatgagag
     2761 gtttccatcg ctcggatgcg gaatgcgcgt ctctactatc gcgagaataa cctgatttcg
     2821 ctcgcgcgat atgacattct tcacattggg gagtcacagg acgaaatgat ggtcagtgcc
     2881 aatctcgtag agcgaatccg ggcgatctgc tgtgttaaag gggtcatcga tgcccatacg
     2941 gagctgtggg gtgatctcgg cctacacgga gacgacgcct tcgagttgct tgaggctatc
     3001 cacacagatt gcggcgtaga tttttccgat ctcgatttca accgtttttt ccccaacgag
     3061 accgagggga tttattactg gtggggccgg cgcatcgggc tgtagcggaa gcgctttgcc
     3121 tccctcacgg tcggtcattt ggatgcggta attgcgcgcc gtcagtggtt cgacccatga
     3181 agcgcacatc gtcgtatgcg atcaccggtt ggaaggccgt tgcgttggct ccttttctca
     3241 tccccctggg gctgatctta actctcttgg ggttcaacaa gaggactatc gagagggctc
     3301 ctgaagatgt ggctggtttc atcgctgaca tgatcgacgg ctcaggcggt gagtgggatt
     3361 gggacgaatt tgaatgcgtg ccgataactg atcccgctct tgacgcgatc cgccttcgcg
     3421 cggccgtctt tgggccgcca aacgcgaaca ttgcggaagc ggaagctata gcggccgcga
     3481 gatccgcatg acagcgccgg gcaggtatcg tgggtaacat gtgggtgggc gcggattaga
     3541 tgcgagattg gcgagatcga ggcgagctta cactggagtt ggaacttatc ttgtccacca
     3601 tcggtcgggc cctcgctgat ttcgaccaaa gcgacttccc ttctttggga aagcagactc
     3661 tgagggtata ggctgagtcg gatgaatgaa ttttagcggc gctgatggcg gcaaggaaca
     3721 aattgagcaa aagcgataaa tcccgagacg acgatgccgc gcccagagtg ctgaaccggg
     3781 cgcagcacgc cgctctggtc aacaaattcc tctcggtatt ggtcaggacc cagctgaaga
     3841 gcggcaaggc tgccggtatc cccaagctgc tggccgagga aatccgcgcc gccggcagcg
     3901 tcgatcgctg gatcaaaagc gatggcgcgc gccgtacgcg ctatgagcag ctcaaggaaa
     3961 agcttggtcc gcgcgccgcg gcattgccct ccggccatgt cgatgcggcc ctggagcgct
     4021 tcctggccat gatcgcggcc atacagatcc gccgcaaaca ggagatctcg attccgcgtc
     4081 ccttcgccaa gctggtgcta gcggccggca gcgtcgaaaa ctggatcgag ggcgacgagg
     4141 caatccgcac gctttacgag gcgacctata agcgcgtcct agtgagcgaa gcggaacggt
     4201 tggcgaaaag gctgcccaga ggcgccgcgc cgccggcccg catcgaaccg cagttgccgc
     4261 atctgccgct ttcgccgcag ggatggggga agccagccgg caaggctgac gaagaatagg
     4321 gtaacgtcta gccggcgcgg ccgataacct cttgcccgat ccgtatgtgc cctaagacgc
     4381 agtggaaagg tccgcataga gcaggtggcc aagagatgca aacgggagat ggaaaaggcg
     4441 cgatagggat acggcgccgg ccgggtgttc gaatcactcc ggctgcacgg cggatgactt
     4501 cccttcattg gtgcctctca cctcgatgcg gattgatgat gtggtctaac gcgacaatgt
     4561 atcggttggc ggtcactcca ggaccgccgt ccgatcggcg gccaagctgc cggtttgcat
     4621 ccccgccatg aatttcccga cactgtcgtg caggcgctgg ttttcggcag taatcgcctc
     4681 attctcgcgc gacagtacgc tcacttgttt cgacagggcg tccgcttcca gggctcgcaa
     4741 atcgagtccg atttggcgca cggcgaaatc gaggacatcc acctcgtcag gccggtaacc
     4801 gtccccttgc ggcttcgggc cgagcaggac gaagccgcct aatacgccgc gatgcatcat
     4861 cggcaaagcg agcgcgcagc cggggatcag tgaaatttcg tcgccgccca gaggggcgtt
     4921 catgtcgcac agggcgatga cttcgcgctc attttccgaa acccactccg gggcggccat
     4981 caggccggat ctcctggccg tcaatccgcc gttccgggcg tcgagataga ttgcgtatcc
     5041 ggcgccgccg gtgaaatccc cgagtacggc gacaaaagca tcgagcagac gctccccatc
     5101 ggtgaaatgg gccgcccggc ggacgaattt ccgcaaagcc gcctctcgcg catgccattc
     5161 atggaacaaa atccgctcga cccaatgttc gacgaagtcg tgcagacggt ggaagctgag
     5221 gaacacgccc agcgcaagtc cggcatcgag caggatgttc tgctcgcggg actcgaaatg
     5281 caggaaatgc tcggctgtcc attccaaaag gccgaagctc gacagcagca gcgcgctggt
     5341 gacgccatag acagcggcgc gattgagcgc gaaaccgaca tcgatcaccc ggtggcgcag
     5401 cacggcatag gccaggatga ggatggcgca ccccagcccg aaagtattgc tgatcgaata
     5461 aaccttccag cccgcgaccg tccagtcgaa agtcgacggc aaggcggcca gcgtcgccat
     5521 gccgaggctg accagcagcc aattgtagcg ggtccgcaga tcgccggaac tgtgggctcg
     5581 tccggccgac agagccacga tcaaaaagag cggcatcgcg atctgctgca gctggcttag
     5641 aggatgcagc ggcggaattt caaagccgta aatgttggcg atttcgtcga tggtgctcgc
     5701 cagacagagc agagccagcg gcggcaatcc gtaacgatgc aggacgcgtc tgatcccctg
     5761 gggacggtcg tcggggaaca gcgtcgcgaa acgcgaagcg gtgtagacca ccatcgagtt
     5821 ggcggcaacc accaggcttc gagtggccag gatagggacg aaggagggaa gaaatctcag
     5881 attcagataa tccgaaaaac acaacatgac gccgatgaaa cacagcgata atgcccggtg
     5941 cgccgaactg ccgggctgac gaaagccgat cagcaggccg acggcgcaga aaatcggacc
     6001 gacgatgagc gagccgagcg cgttgaacgc ggcgtcgggc gcccaaggca agatctcggt
     6061 cgtcacgacg gcgcgatgcc gctcgcttcc atggtgcagg atcaggccga cctcctcgcc
     6121 cgccaccggg tggtagaagt ctttgagatg gtcgaaggtc acgccgtcgc ccacggccgc
     6181 tcccgccctg ctcaacgggc cgtccaaagc gagttgggcg atgatgcgct tggagctgcc
     6241 atactccccg gataacgcaa tgatggcccc aagcgatgcc cgctcctgcc cgacgatcgc
     6301 ggtgagcgcg cccggcaatt ccatcagcaa cagggccgtg gcgagcaagg cgagcagcga
     6361 aatcagaact ttccaatagc gattcgacag acttgacgcc attgcctatg ccctttccgg
     6421 accgcagcaa aaattgtgga tctatttgtt cgcaaaggaa aaatatatct catcttccct
     6481 gtagcgcagg ataccggaca ttgcaaaggg gagatggagg ccgcgtaccg ggaatgaggc
     6541 gggaccgcct atggaccagg cagccggcac cccgtacctt cggcaggggt tggcagccag
     6601 tgccgggccc ttttggccag gtgagcatcc cttcacaccc ttgcggacaa tcgaaggctc
     6661 catgatagtg gtgcgggatg gaatcgcccg ccacttttgg tggcatcggc gtagtcgtgg
     6721 ggggacggat cttgctgata tcgagactcg gcgcggcagc ccgcggccgt gcgggggcgg
     6781 cttcatggtc atgaccgcgg catcggaagg cgatcaccgg gtccagcatt atgcgcgctt
     6841 ctggcaggcg gaggatccgc tggccgatgc ggtcgccgcc gctttcgccg agcgcgccgc
     6901 cggcggcggg cgcgccatgc tcgaaaccgc cctcgaccgg ggcatcggcg ccgtcgacga
     6961 tccgccgccg gcgctcgtcg ccttgtttcg gcagttggac gaggttccct actgggtcga
     7021 ttgggaccgg atcgatcgcg gcagtgcggc cttcctgcgg accggattgc tcggcatggc
     7081 ggtgctcaat ctgttctgcc tgccgatcat gtattattcg ccctgcggca ataagcccct
     7141 ggtttttacc ggcaatcttc tcaagcgggc gccgcgccgc ctggccgaga ccggccgttt
     7201 cgttctcgaa tgcagccgtc ccgatggcct gcgccgcttc tccgacggct tcaagaccaa
     7261 tgtcaaagtc cggctgatgc atgcccaggt caggcggctg ctccggcggt ccgggcgctg
     7321 gaacccgaaa tggggcgcgc cggtcaatca gctctacatg gccggcacca atgtcgcgct
     7381 gtccgccggc ttgatcgagg ggctggagcg gttgggctat cgcatgggcg ccgcggacgt
     7441 cgaagccctt ctgcatctgt ggcgctacac cggctatatt tcgggcatcg cccccgaact
     7501 gcagatatcg acccgcgagg aggggcgtca tctcggcgag ttgatccatc agctcgaagg
     7561 ccagccggac gacgattcgc gcgccctgat caccgccttg atgtcctcct cctaccatcc
     7621 caggctcgag ggcgcgactt ggacgcaaaa gatcaattat ggggtgtcgc gcaagctgat
     7681 cggcaacgag ctggccgatg acctcggcta tccccgctcc cattggggct ggacggtgtc
     7741 gctggcccgg ccgatggccg ggtcgctcgg cctgatccac cgctatgtgc cgcttctgcg
     7801 gtcggcggcg ccgcgctggg gcgaccgcac ctgggacttc atgctcgatc tgatcatggg
     7861 gggaagcaag gcggactatc acgcccccgc gcatctggcc cggccggccc cggaggagaa
     7921 agcggcatga aatcggaaac cttgcccacg ctcagtccgc gcgactggga actgctgcgc
     7981 gagcatgtga cggtgatccg ccggccgcgg ggcgacgtga tcctcgccga gggcggccac
     8041 cgccgtgcca tcttcatcgt caggagcggc gcggtgcggg tcgagcaatc gcagaacggc
     8101 ctcggcatcg cgctcgcgct gctgggcgag ggggaaattt tcggcgagat gggatttgtc
     8161 gaaaacgttc cggccagcgc ctcggtcatc gtgcaggacg acgcggaact cgaagtgatc
     8221 gagggcgacg cgctggaatc gatcaaggcc gccgacccgg gcttttccgt gcgtttctat
     8281 cacagcctgg ccatctcgct ggcgcgtcgg ctgcggctga ccagcgcgcg gctgtcgagg
     8341 gccggcgccg gcgaggcggc ccaggtcaat cgtcaccggg tcgcgcgcac cggcaatatc
     8401 accgcccgcc aagtgccgcc ggcgctcggc gacagcctcg accagttcga gcagaccatg
     8461 ttgtcggtca agctggcgtt gcgcgccggc agcctcgatg cggaagcggc ggttcggcaa
     8521 ttgtcggtca gctgcgacgg ggtggccgcc ctgctgtcgg actataccga ggtcgatccc
     8581 ctggtggaga tcggctacaa cgacctgctg tcctttcgcg accaggacca tctcgaggcc
     8641 ggcatcggcg attacgtgtt ccgcgagacc ttcggcaccc tgctgctgag cgcgaccatg
     8701 gcccggtgct acgccaagcc gcgcggcttt cccgacgatc acgagacgat ggcgatgatc
     8761 gcggcgggcc aggcccaggg cgacgaccgt ctgggccccc tgatcgaccg ctggttcctc
     8821 gaccggccct tatgcctcag ccgccgcgct agcaccgagc tgatgcgccg gagcctggcg
     8881 gaggtggtgg catcccgtcc cgccggcgaa gcgatccgca tcgcgagcct ggcgagcggc
     8941 accgccggcg aattgctcga tctgtgcgcg atctccgacg gggccgcgat cgaggccagc
     9001 ctggtcgatc tcgacggcga ggctctgcag gcagcggcga gacgtgcgga aaggctgggc
     9061 ttgtccgacc gcgtcgtctt ggtgaatggc aatgtcgtgg gcccgcccgg cgaggtggtc
     9121 tcgctcctgc cgcagcacat cgcctatgcc ttgggactgt gcgactacct gaccgacgat
     9181 gaagtggtga tcctgctcga ccgcctgtat gagctggtgg tgccgggcgg ctgcgcgctg
     9241 gtcaccaacc tgtcgcccga caatggcgac cggctgctga tgcagcacct gctcgactgg
     9301 caggcctatc atcgaagcgg ggaccagatc cgcgatttgt tcgcgcggtc ggctttcggc
     9361 gatcgcgcgg tcgagatctt taccgatgcg agcggcgtca ctgtgttcgc gcgctgcgac
     9421 aagtgacgcc gcgacggatt gacaaaacac gccatttccg atgacgggtt tggctaaacc
     9481 tcgatccgag aagaccgcga tttggccgga tttggccatt ggaacggctc ttgctttctc
     9541 tgccctgtgg cctcgggcgc gaaccctcgc gctctcgggt cgcatcgatc aagagaggtg
     9601 tgccatgcgg cttctggttt tattgctgat cctgttcgcc atcttccacg tcgcgctgct
     9661 gatcaaggtg gcgttcgcga tcgtcctggc ggcagcgctg acgatcgggc tttgggtgct
     9721 gtggcgtctg aaatgggtca tcctcggtat tctcggcctc gaagaggtgc tctcggcgcg
     9781 gcggccttaa tccgaggcgc aggtcgaact gtcgatgcag tgatgtattg cgtatgaccg
     9841 gcccgaggcc ggcttcagtc ccgacgacag tatgattgcc aacccatatc acttactgat
     9901 atgagattga gcatgctcga gcccgaccag tctctctcct atctcctcga tttggcgtcc
     9961 ttatcggttc gagaacgccg aaaaattgat taccgatttc ttcagcgaga tcgagcgcgt
    10021 cctccgggag gaaaacaatg gctgaaaaga tcaaagtggg catcgccact catgcggaac
    10081 tcaaggagcg cgcactgcag attgcgcgcg gagagcgccg tcgtcagccc gacgagccga
    10141 agatctggtt cacttcgctg gagtcgctcg cgaagatcct gtcggaggcc aatcggaaat
    10201 tgttgaagat catcgatgag cagcaccctt cttccctggc ggagttggag ctgctcagcg
    10261 gacgcaaggc gagcaatctc tctcgtactc tcaagacgat gagtcaatgt ggcttgatca
    10321 gactcgtccc cggcaagcgc ggttccatcg ctcctgaggt actggtgcgc gaagttcaga
    10381 tggagttgtc catcgtagcg tgagccgcca tgtccgatct
//
LOCUS       DATGEM010000426         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_358114, whole genome shotgun sequence.
ACCESSION   DATGEM010000426 DATGEM010000000
VERSION     DATGEM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358114"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VKQ27_13650"
     CDS             <1..501
                     /locus_tag="VKQ27_13650"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HLY90021.1"
                     /translation="IALLERDRDMIGGGRGACTLARMILVNPLIISTVLGLLWSLSGL
                     PLPAPFGDFVRIFGNTLTGCALFSIGLGFSLSGLRHGWQVSLALSLVKLVVVPGVVWG
                     VAVACGLSRFNTLAAVICAGVPTAKTAYVLAGEFNVEHDLVAATVSLTTLLSAITLLA
                     WLYVLQ"
     gene            586..738
                     /locus_tag="VKQ27_13655"
     CDS             586..738
                     /locus_tag="VKQ27_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90022.1"
                     /translation="MDDDVIVEQGPPASVLDEQTQNRTRRLLRVVDKVEDEAVWQSAG
                     EVCLRG"
     gene            complement(870..1727)
                     /locus_tag="VKQ27_13660"
     CDS             complement(870..1727)
                     /locus_tag="VKQ27_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HLY90023.1"
                     /translation="MPDPIIAAAIQLEPRIGESERNRRHSLEMIERAADQGATLVVLP
                     ELTNSGYVFDSLDEARSLAEHWQTGPSVTAWSRIASARALTIVAGLCEADDDRIYNSA
                     VAIGPNGPLGRFRKVHLWDQENRFFTPGDLGFPVIDTPFGRIGIAICYDGWFPESYRA
                     CALQGADVVCVPTNWVPMPDQPSDREVMANTLVIAAAHSNGLFIIAADRVGTERGQPF
                     LGCSIIAGPTGFPIAGPASPLDEQILLASIDLARAAAARRLNCYNHLLENRRPSQYQA
                     RCPTDSQRL"
     gene            complement(1720..>2838)
                     /locus_tag="VKQ27_13665"
     CDS             complement(1720..>2838)
                     /locus_tag="VKQ27_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY90024.1"
                     /translation="TTAPGVDYPRTDSAVAARLRDAGAILIGKTRTHEYAWGTVTPPV
                     RNPWDLSRIPGGSSGGSGAAVAAGLCPAAMGSDTGGSIRIPASLCGTVGLKPTFGRIS
                     RAGIVPHSWSLDHAGPLTRSVADAALLLQVLAGEDPADPGCSAAPVPDFRAALNEPSA
                     AGLRVAVIRNHFFGRNHPDVDAAVETAIAWFGRQAVIRDVVVPNLDYGLGAIFAIELA
                     SSAAYHDVGLRAGRTSHFTDDVRLLVEMGRLVTGADVIKAEQFRQVLKQDFARVLSEA
                     DIILGPTEPVTAPHIGETHVAVAGGEESALAATWRLTYPYNLTGLPALTLPCGFDPAG
                     LPIGLQIAAAPFAEPILLRAAAAYEAAHGWKGRHPANA"
BASE COUNT          502 a          806 c         1050 g          480 t
ORIGIN      
        1 attgcgttgc tggagcggga ccgcgacatg atcggcggag gccgcggtgc ctgcaccttg
       61 gctcgcatga tcctagtcaa cccattgatc atctcgacag tgcttgggct cctctggtcc
      121 ctgtcagggc tacctctccc cgcaccattc ggagactttg tccgcatctt cgggaacacc
      181 ctgacaggct gcgcactgtt ctcaatcggg ctcggcttct ctctgtcggg cctccggcac
      241 ggctggcagg tttcgctggc gctcagcctg gtgaagctcg ttgtggtgcc cggggtggta
      301 tggggcgttg ctgtcgcgtg cggattgagc aggttcaaca cacttgcagc cgtcatttgc
      361 gccggagtgc caacggcaaa gaccgcctat gtgctcgccg gcgaattcaa tgtggagcat
      421 gacctggtcg cggccacagt ttcgttaacg acgctcttgt ccgccataac tctgctggcc
      481 tggctgtatg tgctgcagtg atgcggcttg gcggcagcag cggcatgctg cccgcttcaa
      541 ggattgcacc cctctggaac cgatggcacc gggaagcagc caagcttgga tgatgacgtg
      601 atcgtcgagc aaggtccgcc cgcttcggtg ctggatgagc agacgcagaa ccgcacgcga
      661 cgcctccttc gcgtggtcga caaggtcgag gatgaagccg tctggcagag tgcgggagaa
      721 gtctgtttgc gtgggtagcg tggcggcagc agcgcgcgct ggcgagcgag caaacgccat
      781 cagtgagtgg ttgatgatgc tggatgggaa ctcatcgaat cgcatgtgcc acccaccaag
      841 ccccgcggca caagtttcgc ggcttgggct tacaacctct gcgagtccgt ggggcaacgg
      901 gcctggtact gggaggggcg gcggttttcc aggagatggt tgtagcaatt gaggcggcgg
      961 gcagcggcgg cgcgggccag atcgatggac gcgagcagga tctgctcatc gagtggcgaa
     1021 gcggggccgg cgataggaaa accggtaggg cccgcgatga tgctgcagcc gaggaaaggc
     1081 tggccgcgtt cggtgccgac gcgatcggct gcgatgatga acaggccgtt ggagtgagca
     1141 gctgcgataa caagcgtatt ggccatgacc tcgcggtccg aaggctgatc cggcatgggc
     1201 acccaattgg tggggacgca aacgacgtcg gcgccttgaa gagcgcacgc acggtacgat
     1261 tcagggaacc agccatcgta gcagattgcg atcccgatac ggccgaacgg cgtatcgatg
     1321 accgggaatc caagatcgcc tggggtgaag aagcggttct cctggtccca tagatggact
     1381 ttgcggaatc ggccgagcgg gccgttcggc ccgatggcga cggcgctgtt gtagatcctg
     1441 tcatcatccg cctcgcataa gccggcgacg atggtcagcg cgcgggcgga ggcgatccgg
     1501 gaccaggcgg tgacggaagg acctgtctgc caatgttcgg cgagggagcg cgcttcgtcg
     1561 aggctgtcga acacgtagcc ggagttggtc agctcaggga ggacaacgag cgtggcgccc
     1621 tggtctgcgg cgcgctcgat catctccagg ctgtggcggc gattgcgctc cgactcgccg
     1681 atccggggtt cgagctggat ggcggcggca atgatggggt caggcatttg cggggtggcg
     1741 ccctttccat ccgtgcgcag cctcgtaggc ggcggccgcg cgaagcagga tcggttcagc
     1801 gaatggggcg gcagcgatct gcaggccgat cggaaggccc gcaggatcga agccacaagg
     1861 gagcgtaagc gctggcagac cggtcaggtt ataagggtaa gtgaggcgcc aggtggcggc
     1921 gagcgcgctt tcctcgccgc cggcgacggc aacgtgggtt tcgccgatat gcggggcggt
     1981 gaccggttcg gtagggccaa ggatgatgtc agcctcgctc aggacgcgcg cgaagtcctg
     2041 cttcagcacc tgacggaact gctcggcttt gatcacatcg gcgccggtga ccaggcgacc
     2101 catctcgacc aggagccgaa cgtcatcggt gaagtgggag gtgcggccgg cacgcagccc
     2161 gacgtcgtga taggcggccg aggaagcaag ctcgatggcg aagatggcgc cgaggccgta
     2221 atcgagattg ggcaccacga cgtcacgaat aacagcctgc cggccaaacc aggcgatagc
     2281 ggtctcaacg gcggcatcga cgtccgggtg gttgcggccg aagaaatggt tgcggatgac
     2341 agcgacgcgc agaccggcgg ccgacggttc gttgagagcg gcgcgaaagt caggaacggg
     2401 tgcggcggag cagccgggat cggcggggtc ctctccggcc aggacctgaa gcagcagggc
     2461 ggcatcggca acactgcggg tcagtggtcc ggcatggtcg agcgaccagc tatgcgggac
     2521 gatacctgcg cggctgatgc ggccgaaggt tggcttcagg ccgacggtgc cgcagaggct
     2581 ggcagggatg cggatggagc cgccggtgtc ggatcccatg gcagcggggc acagtccagc
     2641 ggcgaccgcg gcgccggacc cgccgctgga gccgccggga atgcgggaca ggtcccaagg
     2701 gttgcgaacg ggcggtgtga ccgtgcccca ggcgtattcg tgggtgcggg tcttgccgat
     2761 caggatggca cccgcgtcgc ggaggcgggc ggcgacggca gaatcggtgc gggggtaatc
     2821 tacgccgggc gccgtggt
//
LOCUS       DATGEM010000427        11990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_470712, whole genome shotgun sequence.
ACCESSION   DATGEM010000427 DATGEM010000000
VERSION     DATGEM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470712"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VKQ27_13670"
     CDS             complement(<1..240)
                     /locus_tag="VKQ27_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY90025.1"
                     /translation="MRSTVRLLGMDFTDASTASVAALIAERPPNAPFGYIVTPNADHF
                     VRLHRHPDLWPLYKHAMVRLLDSRVVAKTARTFGLA"
     gene            complement(305..1336)
                     /locus_tag="VKQ27_13675"
     CDS             complement(305..1336)
                     /locus_tag="VKQ27_13675"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HLY90026.1"
                     /translation="MERVDLRSDTVTLPSDPMRLAMAHAPVGDDQYGEDPTVRTLQER
                     VASLLGKEAAVFVPSGTMANQVGLKILTRPGDDVVVGDEAHIVWHESGAAAANSGVQF
                     TVVGRGGLFTAADLRAAYKPPGHIIFPPTTLVAVENTHNRGGGVVFPQADVEAICATA
                     RDLGLATYLDGARLFNTSVASGMSLAALAAPFEVVSVALSKGLGCPVGSLIAGSSAAM
                     RQAVRARRMFGGAMRQSGILAAAGLYALDNNITRLAEDHANARIIGECIAGTRAEIDL
                     ATVQTNIVIFRVPEPLPDAATVVRLAQEQGVLVSAFAARTVRAVTHLNVDAAQCRRAG
                     EVLAKALAG"
     gene            complement(1351..2700)
                     /locus_tag="VKQ27_13680"
     CDS             complement(1351..2700)
                     /locus_tag="VKQ27_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90027.1"
                     /translation="MDRPIIDVTSLIESQKLGRFAVSLLAWTFLCMLLDGFDFAAISF
                     VVPALAREWHVAVGGFGSVFGFGVAGLMVGSIVFGWVGDRIGRRTTIILGCWLFGGFT
                     LASIWAPSVHALLWLRFLAGVGLYAAVPNAIVLVSEFAPRRLRATWVVLMFTGFTIGA
                     VLAGVGGATLIPNFGWQAMFVVGGVLPFVVSICLWFALPDSVRFLTLREHRWGELREV
                     IRRMLPSYRIPADARFVIAEGTREANFTPRLLFAGDLLFITPLLWLFYIFNSAAVFFM
                     QSWMPVLIHGIGLSETNAALTTSVYSLGGTIGGLVTGQIIDRLGLSAVAILPFAGAVV
                     GTLVGVPMPVVALEGLVFGLGFFVVGAQFACTAIAPVFYPTSFRANAEGWALAVAKIG
                     SIAGPMIGGVLLGLHLPGYQLFYFAAIPLVLSAAFAFALGRMARGHLGEAALVTEQV"
     gene            complement(2712..3494)
                     /locus_tag="VKQ27_13685"
     CDS             complement(2712..3494)
                     /locus_tag="VKQ27_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90028.1"
                     /translation="MPEAVPFRGGGFAPRNLPLAGAATLLGAIALWQAGASAGLIPTL
                     FLPAPMNIAIALYHLAISGELWKHLSASLARLAIGWIIGTVFGIGMGLAVGLWSVMRS
                     PGMAIVSALFPIPKIALVPLFIIWFGIGEGSKIITLAFGVFFPTVIATAGGVDNVPRS
                     LIRMGQSFGLSHTAIVRKIVVPAALPAILNSFRVTASIAIVLLVAAEMIGADRGIGAF
                     VLSAGNLYDTDNLLAGIVVLSLLGLAVSWFVGLAERLLLAWR"
     gene            complement(3487..4527)
                     /locus_tag="VKQ27_13690"
     CDS             complement(3487..4527)
                     /locus_tag="VKQ27_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90029.1"
                     /translation="MSTTNGERRAYPHPHVNEGWLAKLREDVIDPALPIVEAHHHLWD
                     RASGVYLLDELRADLAAGHNVRATVFIQCGFAYRSDGPVELRPVGETEHIAGVAARAE
                     AAGLRACAGIVGYCDFRLGEPIDAVLEAHIQAGGGRFKGIRQSAGWDASVVMTTSSPA
                     PAHLLMDPAFRVGLRRLARFGLSYECSLYHPQLPELIDLARALPDQPILVNHCGGPIC
                     VGPYARYPSAAFGNWRQHLQELAKCPNVVLKLGGQAMTIRGFNFHEEVLPPSSGELAA
                     AWKPFMETCIDAFGPERCMFESNFPVDKGMCSYTVVWNAFKRLAAGYSADERAALFHG
                     TAAGFYRLGTIA"
     gene            complement(4606..5388)
                     /locus_tag="VKQ27_13695"
     CDS             complement(4606..5388)
                     /locus_tag="VKQ27_13695"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90030.1"
                     /translation="MSDFGLRLVLSGVTHAYGDLSALEDIDLVAEPGEVTVLVGPSGC
                     GKSTLLGIMGGMLAPTAGRVTCEGEVAPDCLNALTYVFQDFALLPWRTVAGNVALVLE
                     DRLPRAERDARVHEVLALTGLGDFALAWPRQLSGGMRQRVGIARALAVRPACLLLDEP
                     LSALDAQTRDLLLDEFAALIERAGTTTIYVTHNLGEAVRLGHQIVVLSRRPGKVRAIH
                     RIDRPVAGRAVGDADLQAIEAELWRLIRDDAAAAERALDDTA"
     gene            complement(5395..6393)
                     /locus_tag="VKQ27_13700"
     CDS             complement(5395..6393)
                     /locus_tag="VKQ27_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90031.1"
                     /translation="MRFVAVIVALLTSVAAHAADPVRLGLLHTLSPAPFYIAQERGYF
                     RDEGIDLTFRFFEAAQPIAAAAVSGDIDVGVTALTGGFFNLAEKGTLKVIGGGLHEER
                     GYQGSAILVSNQAFDAGLTAPAKLGGHSFAITQYGSSFHYMLGRIAEAEHFDSGSVTL
                     RPVQQIANMVAAVSSNQVDATIAIASMAKPVAAAGQGHIIGWAGDIVPYQITALFTTA
                     PMIAQHADVLKRFARAYQKGVDDYREAFLRLDAQGKPIVDAKTDAVIPLIEKYVFTGD
                     PKAREKIISGVGYYDAGGALDVADVIAQLNWFKAQGLVKGEADPKSVVDTQFLPTR"
     gene            complement(6390..7661)
                     /locus_tag="VKQ27_13705"
     CDS             complement(6390..7661)
                     /locus_tag="VKQ27_13705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90032.1"
                     /translation="MQQQVAVGARTIAITGPRAVAAAVVGNGLEWYNFMLYGVFAGVI
                     GKQFFPASDELTRLLLSFATFSVGFFMRPLGGTLLGMYSDKVGRKAALSLTIMLMCIG
                     TAMIALMPNYAKIGIAAPLLLVVARLLQGFSTGGEMGNATAFMREYAPDGRAGYYVSW
                     VYASSSLFIALGAAVGAIVSNSFSAADLESWGWRVPFLLGLFIGPVGYLLRTRIAEPP
                     EHRRTHQRGGTPFLDVITKHPKEAFASGALITLSTICTYTILIYTPTYATHTLKLPQA
                     AGFTATMVGTITAGVLIPVVGHFVDRIGPKVMLRAAPVLLIVLVYPMFALINAMPSLT
                     TLLVYQIVFGAVIALYQGPILTGIGDMFPNRALATGLGLSDNLAVSIFGGTAALMITW
                     LISLTGSNMVPAYYVMAGGVVGLLGTLPLRR"
     gene            complement(7688..8407)
                     /locus_tag="VKQ27_13710"
     CDS             complement(7688..8407)
                     /locus_tag="VKQ27_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90033.1"
                     /translation="MLEFKDVDTYYGDLHVLKKVNYTIGEGEIVSLLGGNACGKSTTM
                     KTIMGVVRPARGSVVYQGVAIERMPTAERVRRGIAPVLEARRLFPRMTVYENLEMGAY
                     LRKRGAEFDQDLERVYSLFPRVKERRNQIAGTLSGGEQQMVAMGRALMARPKLLCMDE
                     PSMGLAPIFVEQTFDIIQEINKQGTTIFMVEQNANMALSIAHRAYVLQTGQVVLSGTA
                     AELRKNPLIREAYLGEMQVAA"
     gene            complement(8626..10659)
                     /locus_tag="VKQ27_13715"
     CDS             complement(8626..10659)
                     /locus_tag="VKQ27_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLY90034.1"
                     /translation="MNGAAAAAVSVLTRPIARIPEATRRRIGLILLVAFALGFPIFHP
                     NDADVDSMANAAAYATLALGLNIVVGFAGLLDLGYAAFFAIGAYTYGILTAFQLQPEW
                     SSFWEPFQYVGLVAKMPGETGDTSVVHFTLSFWLALPLAGGLAAFFGVLFGAPTLRLR
                     GDYLAIVTLGFGEIVPIVVRNWDSLTNGAAGLNGVAAPRLFGWSFGVDARPYYYVAIA
                     LVAMLVFISIRLRDSRIGRAWMAIREDEIAAGAMGVDRTKFKLMAFAIGAAFGGMTGV
                     FYVAKLQTATPEMFGFPVSVMILVMIVFGGIGSVWGVVLGAFILQLLQSWWLQDLTEW
                     LHALGRLVDNMWLQRVDLVPSIELIFGLILVGMMLFRRQGLIPAVRQQAAMSFDQQHA
                     EVRRGGFTDLKLHGLGGDTRTGAAALEIKGATVKFGGLTALNKVDLVVPNGGVVAVIG
                     PNGSGKSTLFNVITGLVEAESGSIRFHGEELMGLPDYKILEKGVARTFQNLRLFPNLT
                     VMENALIGQHARLKTGALAAILRPPGTVAEEKQAREWAMEIFSIFGNRLTPRAGQMVA
                     GLSYANRRRIEIARALASRPRILLLDEPTAGMNPAETLELAEQVKSLNGLGLTVLLIE
                     HKLDVVTTLADTVYVLDHGEVIAQGKPDEVRRDEEVLRAYLGRNAQAAAAGAA"
     gene            complement(10673..11602)
                     /locus_tag="VKQ27_13720"
     CDS             complement(10673..11602)
                     /locus_tag="VKQ27_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY90035.1"
                     /translation="MTTYDLLLQQLINGLSLGAMYALLALGFTLVYGIMELINFSHFN
                     VFMVGSFIALFVLQSFGLTGQDVILTGLPLIGVLLVAFIVTMVCCGGIGMAIERLLLR
                     RLRDVKGPAAMITTIGISYILFNVILLTVGADSKNFPNPLPPIRYHIGEAVLDLRVIL
                     IWIVALLLMAGLYVFVQRSRIGKAMRATAQDAEAARMMGVEVDKVILMAFFLGSALAG
                     AGGLIFGLYYNFTSFIIGFTAGLRAFTAAVLGGIGNVPGAMVGGVLIGLIEAMASQFI
                     AAAWADVIIFSILVLVLVFRPAGLFGRVAPSKS"
     gene            complement(11607..>11990)
                     /locus_tag="VKQ27_13725"
     CDS             complement(11607..>11990)
                     /locus_tag="VKQ27_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90036.1"
                     /translation="PAANGWYATIASPHTVADNKAQAFVDAYFRKYSKAADDYTITAY
                     DATLVVIDAIRRVAASGKPINRSAVRDAIQSSKVSTIQGIVQFDDNGDLKDRTVSVFQ
                     IKEDKSARPDDVSRQYHYIGVAPQV"
BASE COUNT         2483 a         4326 c         3331 g         1850 t
ORIGIN      
        1 tgccaggccg aaggtccgcg ccgtcttcgc cacgacccgc gaatccagca gacgcaccat
       61 tgcgtgctta tagagcggcc acagatcagg atgccgatgc aggcgcacga aatggtccgc
      121 gttgggggta acgatgtagc cgaacggcgc attaggcggc cgttcagcaa tcagcgccgc
      181 aaccgatgca gtgcttgcat cggtgaaatc catcccaagc aggcgcacag tgctccgcat
      241 gcacctgagt caacgcgcaa atgcttaaag aactctgctg gacgcgtacc gctcggcgat
      301 caattcaccc agccaaagcc ttggccagca cctcacccgc ccggcggcat tgcgcggcgt
      361 caacgttcag atgtgtgacc gcccgcacgg tgcgcgccgc aaaggctgaa acaagcacgc
      421 cctgttcctg tgccagcctg accacagtcg ccgcatcagg gagtggctcc ggcacgcgga
      481 agatcacgat gttggtctgc actgtcgcga ggtcgatctc ggcccgggtc ccggcgatgc
      541 attcgccgat gatgcgtgcg ttggcatgat cctcggccag gcgcgtgatg ttgttgtcga
      601 gcgcatacag ccccgccgcc gccaggatgc ccgactgacg catcgcacca ccaaacatgc
      661 gcctggcgcg caccgcctgc cgcatcgctg ccgaactgcc ggcgatcagg ctgcctaccg
      721 ggcatccaag ccctttcgac agggccacgg acacaacctc gaatggcgcg gctagggcgg
      781 caagcgacat gccgctcgcc accgatgtgt tgaacagccg cgcaccatcc aggtaggtgg
      841 caagccccag atcgcgcgcg gtcgcgcaga tcgcctcgac gtcggcctga ggaaacacca
      901 cgccgccgcc gcggttgtgc gtgttctcaa ccgccacgag agttgtcgga ggaaagatga
      961 tgtgcccggg cggcttatat gccgcacgca gatccgcggc cgtgaacagg ccgccccgcc
     1021 ccaccacggt gaactgcaca ccggagttcg cggctgccgc gccagactcg tgccagacga
     1081 tatgcgcttc gtcacccacc acgacatcgt cgccagggcg cgtcaggatc ttcaggccaa
     1141 cctggttcgc catcgtgccg ctcggcacga ataccgctgc ttccttgccc aacagcgacg
     1201 cgacacgctc ctgtaacgtg cgcaccgtcg gatcttcgcc atactgatca tcgcccactg
     1261 gcgcgtgcgc catcgccagg cgcatcggat cgcttggcag cgtcacagta tcagaccgca
     1321 ggtcgacccg ttccatcggc gcctccttcc tcacacctgc tcagtgacga gcgcagcctc
     1381 accgagatgg ccccgtgcca ttcgcccaag cgcgaatgcg aacgccgcac tcagcaccag
     1441 cggtatcgcc gcgaaataga acagctggta gcccggcagg tgcagcccca gcagcacgcc
     1501 gccaatcatt ggccccgcga tcgagccgat ctttgccacc gccagcgccc agccctcggc
     1561 attcgcgcga aacgaagtgg gatagaacac cggggcaatt gccgtgcagg cgaactgcgc
     1621 acccaccacg aaaaacccga gcccgaacac cagcccttcc agcgcgacca ccggcatcgg
     1681 cacgccaacc agcgtgccga caaccgcacc ggcaaacggc aggattgcga ccgcggaaag
     1741 ccccagccgg tcgatgatct gccccgtcac caggccacca atggtgccac cgagtgaata
     1801 caccgatgtc gtcagtgccg catttgtctc ggagaggccg atgccgtgga tcagcaccgg
     1861 catccagctc tgcatgaaga acaccgcggc cgagttgaag atgtagaaca gccagagcag
     1921 cggcgtgatg aacagcaggt ccccggcgaa cagcagccgc ggcgtgaagt tcgcctcgcg
     1981 cgtcccctcg gcaatcacga accgcgcatc ggcaggtatc cggtagctcg gcagcatgcg
     2041 ccggatcacc tcgcgcaatt cgccccagcg atgctcgcgc agcgtcagga agcgaaccga
     2101 gtcgggcaac gcgaaccaca ggcagatcga caccacgaat ggcagcaccc cacccacgac
     2161 aaacatcgct tgccagccga aattcgggat cagcgtagcg ccgccaacgc cggccagcac
     2221 ggcgccgatg gtgaagcccg tgaacatcag caccacccag gtggcgcgca gccgccgcgg
     2281 cgcaaattcg ctcaccagca cgatcgcatt cggcaccgcc gcatacagtc cgacaccggc
     2341 caggaagcgc agccacagca aggcatgtac cgacggcgcc catatcgacg ccagcgtgaa
     2401 tcctccgaac agccagcatc ccagaatgat ggtcgtgcga cggccgatgc ggtcgccgac
     2461 ccaaccgaac acgatcgatc ccaccatcag cccggcgaca ccgaagccga acacggaacc
     2521 aaagccgcca accgccacgt gccattcacg cgccagcgcg gggacaacaa aggaaatcgc
     2581 cgcgaagtcg aaaccatcga gcagcatgca gaggaacgtc cacgcaagca ggctcaccgc
     2641 gaagcgtcca agcttctggc tctcgatcag cgacgtcacg tcaatgatgg gtcgatccat
     2701 gctggctcct gtcaccgcca cgcaagcagc agccgctccg cgaggccgac gaaccacgat
     2761 accgcaagtc ctagcagcga caacacgacg atgccggcca gcagattgtc cgtgtcgtaa
     2821 aggttgccgg ccgacagcac gaacgcgccg atgccgcggt ccgcgcctat catctcggcc
     2881 gccaccagca gcacgatcgc gatcgatgcc gtgacccgga agctgttcag gatcgccggc
     2941 agggcggccg gcaccacgat cttccgcacg atcgccgtgt gcgacaggcc aaagctctgc
     3001 cccatgcgga tcagcgagcg cggcacgttg tccaccccgc cggccgtcgc gatcaccgtc
     3061 gggaagaaca cgccgaacgc cagtgtgatg atcttcgagc cctcgccgat accgaaccag
     3121 atgatgaaca gcggcaccag cgcgatcttc gggataggaa acagcgccga cacgatcgcc
     3181 atgccaggcg aacgcatgac cgaccacagg ccaaccgcga gccccatgcc gatgccgaac
     3241 accgtaccga tgatccagcc gatcgccagc cgcgcgagcg acgcgctcag gtgtttccac
     3301 aactcgccgc tgatcgccaa atgatacagc gcgatcgcga tgttcatcgg cgccggcagg
     3361 aacaatgtcg ggatcagccc cgccgaggca cctgcctgcc acagcgcgat cgcacccagc
     3421 agtgtcgcag ccccagccag cggcaggttg cgcggcgcaa acccgccgcc cctgaacggg
     3481 accgcctcag gcaatggtgc ccagccgata gaagcccgcg gcggtgccat ggaacagcgc
     3541 cgcccgctcg tccgccgaat acccggccgc cagacgcttg aaggcattcc agaccaccgt
     3601 gtagctgcac atgcccttat cgacagggaa gttgctctcg aacatgcagc gctccggccc
     3661 gaatgcgtcg atgcaggttt ccatgaacgg cttccacgcc gcggcgagtt cgccagatga
     3721 cggcggcagc acctcctcgt ggaaattgaa gccacgtatc gtcatcgcct gcccgcccag
     3781 cttcagcacc acattggggc acttcgccaa ctcctgcaga tgctgtcgcc agttgccgaa
     3841 ggcagcgctc ggatatcgcg cgtaagggcc aacgcagatc ggtccgccgc aatgattgac
     3901 caggatcggc tgatccggca gcgctcgcgc gaggtcgatc agctcgggca gttgcggatg
     3961 atatagcgag cattcgtatg acagcccgaa cctggccagc cgccgcagcc ccacgcgaaa
     4021 cgccggatcc atcagcaggt gcgctggcgc cggcgaggac gtcgtcatca ccaccgacgc
     4081 atcccatccg gcgctctgcc gtatgccctt gaagcggccg ccgccggcct ggatatgcgc
     4141 ctccaacacc gcatcgatcg gctcgcccag cctgaaatcg cagtaaccga cgatacccgc
     4201 acaggcgcgc aaccctgccg cttcggcgcg cgcggcgacg ccggcgatat gctcggtctc
     4261 gcccaccggc cgaagctcaa ctggcccgtc actgcgatag gcgaagccgc actggatgaa
     4321 cactgttgcc cgcacgttat gtccggccgc cagatcagcg cgcagctcat cgagcagata
     4381 aacacccgac gcccgatccc aaagatgatg gtgcgcttcc acgatcggca gcgccggatc
     4441 gatcacgtcc tcgcgcagtt tcgccagcca gccctcgttc acatgcggat gcggataggc
     4501 ccggcgttca ccgttcgtcg tgctcatgac gctctccccg atcgcttgtg ccgcagcgta
     4561 gaccgtcccg ccataccgga aaatgcgaca gacccacggc tccggtcatg ccgtatcgtc
     4621 gagcgcgcgc tccgccgccg ccgcatcgtc acggatcaac cgccacagct cggcttcgat
     4681 cgcctgcaga tcggcatcgc ccactgcgcg tcccgccacc ggccgatcga tccggtggat
     4741 cgcacgcacc ttgcccggcc gccgcgacag cacaacgatc tgatgaccca gccgcacggc
     4801 ctcgccgaga ttgtgcgtca cgtatatcgt cgtggttccg gcgcgctcga tcagcgcggc
     4861 gaactcatcc agcaacaggt cccgcgtctg cgcatccagc gccgatagcg gttcgtccag
     4921 caacaggcac gccggccgca ccgccagtgc gcgcgctatc ccaacccgtt gccgcatgcc
     4981 gcctgacaac tgccgtggcc acgccaaggc aaaatctccc agacctgtaa gcgccagcac
     5041 ctcatgcacg cgcgcgtcgc gctcggcgcg cgggagccga tcctccagca ccagcgcgac
     5101 attgcccgcc acggtacgcc atggcagcag cgcaaaatcc tggaacacat aggtcaacgc
     5161 attcaggcag tccggggcaa cctcgccttc gcaggtcact ctgccggcag tcggtgccag
     5221 catgccaccc atgatgccca gcagcgtcga cttgccgcag cccgacggcc cgaccaggac
     5281 ggtgacctca cccggctctg cgacgagatc gatgtcctcc agtgcgctca aatcgccata
     5341 ggcatgggtg acgccgctca gaaccagacg caggccgaag tctgacatga tccatcagcg
     5401 cgtcggcagg aactgcgtat cgaccaccga cttcggatcc gcctcgccct tcaccagccc
     5461 ctgcgccttg aaccagttga gctgcgcgat cacgtccgcc acatcgagcg ccccgcccgc
     5521 atcataatag ccaaccccgc tgatgatctt ctcgcgcgcc ttcggatcgc cggtgaacac
     5581 gtatttctcg atcagcggga tcaccgcatc ggtcttcgca tccacaatcg gcttgccttg
     5641 cgcatccagc cgcaggaacg cttcccgata atcgtcaaca cccttctgat acgcacgcgc
     5701 gaaccgcttc agcacgtcag catgctgtgc gatcattggt gccgtcgtga acagcgcggt
     5761 gatctgatac ggcacaatat cgcctgccca gccgatgata tgcccttgcc ccgccgccgc
     5821 aaccggcttc gccatcgagg caatcgcgat cgtcgcatcc acctggttgc tgctgaccgc
     5881 cgccaccatg ttggcgatct gctgcaccgg ccgcagcgtc accgagccgc tatcgaaatg
     5941 ctccgcctcg gcgatccgcc ccagcatgta gtggaacgac gaaccatact gcgtgatggc
     6001 aaagctgtgt cccccaagct tcgccggcgc agtaagcccc gcatcgaatg cctggttcga
     6061 aaccagtatg gcggatccct ggtaaccgcg ctcctcatgc agtccgccgc cgatgacctt
     6121 cagcgtcccc ttctccgcca ggttgaagaa cccgccggtc agcgcggtca cacccacatc
     6181 gatgtcgccc gacacagcgg cagcagcgat cggctgcgcc gcctcgaaga agcggaacgt
     6241 caggtcgatc ccttcatccc ggaaatagcc acgctcctgc gcgatataga atggcgccgg
     6301 cgacaatgtg tgcagcagtc ccagtcgcac cgggtccgcc gcatgcgccg cgacactcgt
     6361 cagcagcgca acgataaccg caacgaacct catcggcgca gcggcaaagt gccaagcagg
     6421 ccaaccacgc cgcctgccat cacataatac gccggcacca tgttgctgcc cgtcagcgag
     6481 atcagccatg tgatcatcag tgccgctgtg ccgccaaaga tcgacaccgc cagattgtcc
     6541 gaaagcccca gccctgtcgc cagcgcccgg ttcgggaaca tgtcgccaat gcccgtcagt
     6601 atcggaccct ggtacagcgc gatcaccgca ccgaacacga tctgatagac cagcagcgtc
     6661 gtcaggctcg gcatcgcatt gatcagcgcg aacatcggat acaccaggac aatcagcaga
     6721 accggcgccg cccgcaacat caccttcggc ccgatccgat cgacgaagtg tcccaccacc
     6781 gggatcagga cgcccgcagt gatcgtgccg accatcgtcg cggtgaaccc cgccgcctgc
     6841 ggcagcttca gcgtgtgcgt cgcatatgtc ggcgtataga tcaggatcgt gtaggtgcag
     6901 atcgtggaaa gcgtgatcag cgcgcccgag gcgaacgctt ccttcggatg cttggtgatc
     6961 acatccagaa acggcgtacc gccgcgctga tgcgtacgcc tgtgctccgg tggttcggca
     7021 atgcgcgtgc gcagtagata accgaccgga ccaatgaaca gcccgagcag gaacggcacg
     7081 cgccaccccc agctctccag atcggccgcg ctgaagctgt tggacacgat ggcgccgact
     7141 gccgccccca acgcgatgaa caggctgctc gaggcataca cccagctcac ataataaccc
     7201 gcacgcccat ccggcgcgta ttcgcgcatg aaggccgttg cgttgcccat ttcgccgccg
     7261 gtcgaaaagc cctgcagcag ccgcgccacc acgagcagca gcggcgccgc aataccaatc
     7321 ttcgcgtagt tcggcatcaa tgcgatcatc gcggtgccga tgcacatcag catgatggtc
     7381 agcgacagcg cggccttgcg cccgaccttg tcggaataca tccccagcag tgtgccgccc
     7441 agcgggcgca tgaagaaccc gacgctgaac gtggcgaacg acagcagcag ccgcgtcagt
     7501 tcgtccgatg ccggaaagaa ctgcttgccg ataacgccgg caaacacgcc atacagcatg
     7561 aaattgtacc actcgaggcc gttgccgacg acggcggcgg caacggcgcg cggtccggta
     7621 atggcgatgg ttcgcgcgcc aacggcaacc tgctgctgca tatcgaactc ccccgttcga
     7681 ggagagctta ggccgccacc tgcatttcgc ccagataggc ctcgcggatc agcggattct
     7741 tccgcagttc ggccgcagta cccgacagca ccacctgtcc cgtttgcagc acataggcac
     7801 gatgcgcgat ggacagcgcc atgttggcgt tctgctccac catgaagatc gttgtgccct
     7861 gcttgttgat ctcctggatg atatcgaacg tctgctcgac aaagatcggc gccaggccca
     7921 tcgacggttc gtccatgcaa agcagcttcg gcctcgccat cagcgcgcgc cccatcgcca
     7981 ccatctgctg ctcaccaccg gacaatgtcc cggcgatctg attgcgccgc tccttgaccc
     8041 gagggaacag cgaatagacc cgctccagat cctgatcgaa ctccgcgcca cgcttacgta
     8101 gatacgcgcc catctccagg ttctcgtaca ccgtcatgcg agggaacagc cgccgcgcct
     8161 ctagcaccgg cgcgatgcca cgacgcacgc gctccgcagt cggcatacgc tcgatcgcca
     8221 cgccctgata aaccacgctc ccgcgcgccg gccggaccac gcccatgatc gtcttcatcg
     8281 tggtggactt gccgcaggca ttgccgccca gcagagagac gatctccccc tctccgatcg
     8341 tgtagttcac cttcttcagc acatgcaggt cgccgtagta ggtgtcgaca tccttgaatt
     8401 cgagcagcat ctcagtcctc atttccaacg gctgagccac gatccgcatc ggcaggaata
     8461 acacggatta ccctgattgg ctcgctgcgc gatccactgc gcggattgca cggaaagtat
     8521 tttgtgcgct tcacgccgcc accaaccagc acctgcgcga agccccaaaa attctttctt
     8581 tccgtccaat ccgtgcatca atccgtgtca tcggtgttaa agcctctaag cagcccccgc
     8641 cgccgcggcc tgcgcattgc ggcccagata tgcccgcagc acctcctcat cgcgccgaac
     8701 ctcatctggc ttgccctgtg cgatcacctc gccatggtcc agcacataca ccgtatcagc
     8761 cagtgtcgtg accacatcca gcttgtgctc gatcagcagc acagtcagcc ccaggccatt
     8821 caggctcttc acctgctccg ccagctccag cgtctccgcc ggattcatcc ccgccgtagg
     8881 ctcatccagc agcaatatcc gcggccgcga cgccagcgcc cgagcgatct cgatgcgccg
     8941 ccgattggca taggacagtc ccgcaaccat ctgccccgcc cgcggtgtca ggcgattgcc
     9001 gaagatcgaa aaaatctcca tcgcccactc gcgagcctgc ttctcctccg caaccgtccc
     9061 cggcggacgc aggatcgccg cgagcgcacc cgtcttcagc cgcgcatgct gaccaatcag
     9121 cgcgttctcc atcaccgtca ggttcgggaa caaccgcaaa ttctggaacg tgcgggcgac
     9181 gcccttctcc aggatcttgt agtcaggcaa ccccatcagc tcctcgccat ggaaacggat
     9241 cgagcccgac tcagcctcca ccagcccggt gatcacgttg aacagcgtgg acttgccgga
     9301 tccgttcggc ccgatcactg ccaccacacc gccgttcggc accaccagat caaccttgtt
     9361 cagcgccgtc agcccgccga acttgacggt cgcgcccttg atctccagcg ccgccgcgcc
     9421 agtgcgcgta tcaccaccca gcccatgcag cttcagatcg gtgaagccgc cgcgccgcac
     9481 ttcggcatgc tgctggtcaa agctcatcgc cgcctgctgt cgcaccgcgg ggatcagccc
     9541 ttgccgccgg aacagcatca tccccaccag gatcaagccg aagatcagct cgatcgacgg
     9601 caccaggtcc actcgctgta gccacatgtt atcgaccagc cggcccagcg catgcagcca
     9661 ctccgtcagg tcctgcagcc accacgattg cagcaactgc agaatgaacg cgcccagcac
     9721 cacgccccaa acactgccga tgccgccaaa tacgatcatc accaggatca tgacggatac
     9781 cgggaagccg aacatctcgg gcgtcgccgt ctgcagcttc gcgacataga aaacgccggt
     9841 catgccaccg aacgccgcac cgattgcgaa tgccatcagc ttgaacttgg tccgatcgac
     9901 gcccatcgcg ccggctgcga tctcatcctc ccggatcgcc atccacgccc ggccaatgcg
     9961 cgaatcccgc aatcggatgg aaatgaaaac cagcatcgcc accagcgcga tcgcgacata
    10021 gtagtacggc cgcgcatcga cgccgaagct ccagccgaac agcctcggcg cagccacgcc
    10081 gttcaacccc gccgcaccgt tcgtcaggct gtcccagttg cgcaccacga tcggcacgat
    10141 ctcgccgaag cccagcgtca cgatggccag gtaatcaccg cgcagccgca gcgtcggcgc
    10201 accgaacagc acgccgaaga atgccgccag cccgcccgca agcggcaacg ccagccagaa
    10261 cgacagcgtg aaatgcacga cgctggtgtc accggtctcg ccgggcatct tcgccaccag
    10321 ccccacatac tggaacggct cccagaagct gctccactcc ggctgcagct ggaacgcggt
    10381 caggatgcca tacgtatagg ctccaatggc gaagaacgcg gcgtagccaa gatccagcag
    10441 ccccgcgaag cccaccacga tgttcagccc gagcgcgagc gtcgcatacg ccgccgcgtt
    10501 ggccatgctg tccacgtcgg catcgttggg gtggaagatc ggaaagccca acgcgaacgc
    10561 caccagcaag atcagcccga tacggcgtcg ggtggcctcc gggattcgcg caatcggtcg
    10621 cgtcaggacc gagacagcgg cggctgcggc gccattcatc cgcggtcccc tttcacgact
    10681 tgctgggcgc gacgcgcccg aacagaccag cgggcctgaa caccagcacc agcaccagga
    10741 tcgagaatat gatcacgtcg gcccaggccg cggcgatgaa ctggctcgcc atcgcctcga
    10801 tcagtccgat cagcacgcca cccaccatcg cgccaggcac gttgccgatg ccgcccagca
    10861 ccgcggcggt gaaggcgcgc agtccagcag tgaagccgat gatgaagctg gtgaaattgt
    10921 aatacaggcc gaagatcagc ccgccggctc cggcaagcgc cgagccaaga aaaaaggcca
    10981 tcagaatgac cttgtccacc tcaacgccca tcatgcgtgc agcctccgcg tcctgggcgg
    11041 tcgcacgcat cgccttcccg atacggctgc gctgcacgaa cacgtagagg cccgccatca
    11101 acagcaacgc gacgatccag atcaggatga cgcgcaaatc gagcactgct tcgccaatgt
    11161 gataacggat cggcggcaac ggattgggga agttcttcga gtctgccccc acggtgagca
    11221 ggatgacatt gaacaggata tacgagatgc caatggtggt gatcatcgcc gccggcccct
    11281 tgacgtcgcg caacctccgc aacaacagcc gctcgatcgc catgccaatc ccgccgcagc
    11341 acaccatcgt cacaataaag gcgaccagca gcacaccaat gagcggcaac ccggtcagta
    11401 tgacgtcctg gccggtcagc ccgaaggact gcaacacgaa cagcgcgatg aacgatccca
    11461 ccatgaacac gttgaagtgg gagaagttga tcagctccat gatgccgtag accagggtga
    11521 acccaagtgc gagcaacgcg tacatcgcac ccaggctcaa cccattgatc aactgctgca
    11581 gcagcaggtc gtaagttgtc atctgatcag acctgcggcg ccacgccgat gtagtgatac
    11641 tgacgagaca cgtcgtccgg ccgcgccgac ttgtcctctt tgatctggaa tacgctcacg
    11701 gttcgatcct tcaaatcacc attgtcatcg aactgcacga tgccctggat cgtcgaaact
    11761 ttcgacgact ggatcgcatc gcgcacggcg gaacgattga ttggcttgcc gctcgccgcc
    11821 acccgccgga tcgcatcgat aaccaccaaa gtcgcgtcgt acgcagtgat cgtatagtcg
    11881 tccgccgcct tgctgtattt gcggaaatac gcatcgacga aggcctgcgc cttgttgtcg
    11941 gccaccgtgt gcggcgaggc gattgtcgcg taccagccat tcgccgccgg
//
LOCUS       DATGEM010000428         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1584609, whole genome shotgun sequence.
ACCESSION   DATGEM010000428 DATGEM010000000
VERSION     DATGEM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584609"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VKQ27_13730"
     CDS             <1..586
                     /locus_tag="VKQ27_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90037.1"
                     /translation="SFLVQAQSSGAKVVGFANAGVDLQNCLKQAAEFGLTANGTRLAT
                     LLMQISDVHSLGQKVCAGLAYTDSFYWDLNDKTRAWSKRWGAKMNGMVPGLLHAGSYC
                     AAYHWLKAVKAAGSTDADAVAAKMKAIPVNDFYNDNVKIREDGRVMHVMYLWQVKAAS
                     ESKYPFDYCKSLAIISPDQAWRPMSEGGCPLVHA"
     gene            complement(706..2814)
                     /locus_tag="VKQ27_13735"
     CDS             complement(706..2814)
                     /locus_tag="VKQ27_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY90038.1"
                     /translation="MSDTLPLSIQNNRRMEKWIRFDADRTVRLAVGKVELGQGNVTAL
                     AQIAADELDVDLGRIAVLSGDTQDAPDEGQTTGSQSIEVSGSSVRLVSAELRSRVLDR
                     LAQRLNCSPAELSVEDGAFHRAGAPTGHDYWNFAAAEDFTRDIEGSAKPKPHTAYRYV
                     GRSTPRRDLPAKISGGAFVHDMVRPDTLHARILRQPCRGAKLASFDEAAVRRAERGDF
                     RVIRVGDFVAFVGSDEGVVQRAAAVAPMHAKWQNVRRITPEQQEAAWLVGQPSIDRVL
                     GDPLPAATTTNVVEASYSRPYVAHASMAPSCALAEYRNGHLSVWSHTQGPYPLQSALS
                     SVLGIPQDTITVRHAHGAGCYGHNGADDAAVDAAVIALQVPDHCIRVQWRREEEFGFE
                     PVGPAMHITLRAALDDAGKPVDWTAEIWSPTHVQRPSSGGNMLTHEALPTPPPDPRPT
                     DPPEANGGGGTRNAFPLYGFAARRVIHHLVRQAPVRTSALRGLGALPNVFAIECFMDE
                     LAERAGVDPVEYRLSAITDPRARVLVETLAKRCNWAARGPAGTGRGLGLGWAKYKNKA
                     AYAGVALEVEVEQEVRVSRVWCVADAGLVINPDGACNQLDGGIVQAISMTLKEQVRME
                     GDGITSLDWSGYPILHFSEVPEIETEIIPMPDHPTLGMGECTFGPTAAAIGNAVAHAL
                     GARIRDMPFTREKIAAALLK"
     gene            complement(2811..3284)
                     /locus_tag="VKQ27_13740"
     CDS             complement(2811..3284)
                     /locus_tag="VKQ27_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY90039.1"
                     /translation="MIELTVNGRPVQTAMAGTTPLLDVLRNQLGLLGTRYGCGLEQCG
                     SCVVLLDGELVHACSREVGTAAGGRVTTIEGLGTPAAPHPLQQAFLDEQAGQCGYCLS
                     GIIMSAKALLDRNPSPSRGEIVRALDKHLCRCGAQPRILRAVEKAAAMMQGGKGA"
     gene            complement(3281..>3794)
                     /locus_tag="VKQ27_13745"
     CDS             complement(3281..>3794)
                     /locus_tag="VKQ27_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY90040.1"
                     /translation="KTLAPALRVGYAVSDWPVLSRMLACKTDSGTGALDQMIVAEYFA
                     NHFDRHVGALTNVLKEKLDTMIEAVEREFGTSAEMWKPKGGIFLWFKLPDSVDVRKLV
                     KPAADAGIQFNPGPEWVCNPEPAKSYMRLCFALPSKEVIREGVAALAQVCYEQTGIPV
                     RSANVQRRTA"
BASE COUNT          693 a         1274 c         1154 g          673 t
ORIGIN      
        1 atcgttcctt gtgcaggcgc aaagctcagg cgcgaaggtc gtgggcttcg ccaatgcggg
       61 cgtcgatttg cagaattgcc tgaagcaggc tgcggaattt ggcctcacgg ctaatggcac
      121 gcggctggcg acgctgctga tgcagatcag tgacgtgcac tcgctcggcc agaaggtgtg
      181 cgccggattg gcctatacgg actcgttcta ctgggacctg aacgacaaga cgcgcgcctg
      241 gtcgaagcgc tggggtgcca agatgaacgg gatggtgcca ggcctgctgc atgccggcag
      301 ctactgcgcc gcctatcact ggctgaaggc ggtcaaggcg gcgggctcga ccgatgccga
      361 tgcggtggcg gccaagatga aggccatacc ggtcaatgac ttttacaatg acaacgtgaa
      421 gatccgtgag gacggccgcg tgatgcacgt gatgtatctg tggcaggtga aggccgcgtc
      481 ggagtcgaaa taccccttcg attactgcaa aagtctcgcg atcatctcgc cagaccaggc
      541 ctggcgtccg atgagcgagg gcggctgccc gctggtacac gcgtagcgtc tcacgtacct
      601 tgtcattgcg accctggcga aggccggggg aagcaatctc gtccggaatt gcttggccgg
      661 gtcaagcgcc ggccaccgct ccgctcctcg caatgacagg tgtagctact tcaatagcgc
      721 cgccgcgatc ttctcccgtg taaacggcat gtcgcgtatg cgtgcgccga gcgcgtgggc
      781 caccgcgttg ccgatcgcgg cggcagttgg cccgaaggtg cattcgccca taccgagcgt
      841 cggatggtcc ggcatcggga tgatctccgt ctcgatctcg ggcacctcgg aaaaatgcag
      901 gatcggataa cctgaccaat cgagcgacgt gatgccgtcg ccctccatcc tcacctgctc
      961 cttcagcgtc atgctgatcg cctgcacgat gccgccatcg agctggttgc atgcaccgtc
     1021 cggattgatc accagcccgg catctgcaac gcaccagacg cggctcacgc gcacttcctg
     1081 ctccacctcc acttccagcg cgacgccggc ataggcggcc ttgttcttgt atttcgccca
     1141 gccgaggcca agccctcgcc cggtgccagc aggaccacgc gcggcccagt tgcaacgttt
     1201 cgccagcgtc tccaccagca cacgcgcccg gggatcggtg attgcggaca accggtactc
     1261 aaccggatcg acaccggcac gctccgccaa ctcatccatg aagcactcga tggcgaacac
     1321 gttcggcaga gcaccgagcc cacgaagtgc cgatgtacgc acgggcgcct gccgcaccag
     1381 atgatgaatc acgcgccgcg ccgcgaagcc atacagcggg aacgcattgc gcgtaccgcc
     1441 gccgccattc gcctccggcg gatcagtggg acgcggatcg ggcggaggcg tcggcaatgc
     1501 ctcgtgagtg agcatgttgc cgccgctgct gggccgctgc acgtgcgtcg gtgaccagat
     1561 ttccgcagtc cagtccacgg gctttccagc atcgtccaaa gctgcccgca gtgtgatgtg
     1621 catcgccgga ccgaccggct cgaagccgaa ctcttcctcg cgccgccact gcacgcggat
     1681 acaatgatcc ggcacctgca atgcgatcac agcagcatcc acggcagcat cgtcagcacc
     1741 gttgtggccg tagcatccag cgccatgcgc atgtcgcaca gtgatcgtgt cctgcgggat
     1801 acccagaacg ctcgacaggg cggattgcag tggatacggc ccctgtgtgt gcgaccagac
     1861 cgacagatgg ccattgcggt attcggccaa cgcacaggat ggcgccatgg atgcatgcgc
     1921 aacgtatggc cgcgaatagc ttgcctcgac cacgttcgta gttgttgccg caggcagcgg
     1981 atcgcccagc acacgatcta tggaaggctg gccgaccagc caggccgctt cctgctgttc
     2041 gggagtgatc cgccgaacgt tctgccattt cgcgtgcatc ggtgccacgg cagctgcgcg
     2101 ctgcaccacg ccctcatccg aaccaacgaa ggcaacgaaa tcaccaacac ggatcacgcg
     2161 gaaatcgcca cgctcggcgc gccgcactgc tgcctcatca aacgaggcga gcttcgcgcc
     2221 gcggcagggc tgacgcagga tccgggcgtg cagcgtgtcg gggcgcacca tgtcgtgcac
     2281 aaaggcaccg ccggagatct ttgccggcag atcgcggcgc ggcgtcgacc gcccgacata
     2341 gcgataggca gtgtgcggct tcggctttgc actgccttcg atgtcgcgtg tgaaatcttc
     2401 ggcggctgcg aagttccagt agtcatgtcc ggtcggtgcg ccggcacgat ggaacgcccc
     2461 atcctcaacc gacagttccg ccgggctgca attcagccgc tgcgcaagtc gatccagcac
     2521 gcgtgaccgc agctctgccg agaccaggcg aacggaactg ccggagacct caatcgactg
     2581 gctgccggtg gtctgccctt catccggcgc atcctgtgta tcgccagaca gcacggcgat
     2641 ccggccgaga tccacgtcca gttcgtcggc ggcaatttgc gccaatgcag taacgtttcc
     2701 ctggccgagc tcgactttgc cgaccgcgag ccgcacggtg cgatccgcgt cgaagcggat
     2761 ccacttctcc atgcgccggt tgttctggat cgacagtggc aatgtgtcgc tcatgccccc
     2821 ttccctccct gcatcatggc agcggctttt tcgacagcgc gcaggatccg cggctgcgca
     2881 ccgcagcgac agagatgctt gtccagcgct cgcacgatct ccccgcggga tggcgatgga
     2941 ttgcggtcca gcaatgcttt cgcactcatg atgatgccgg agaggcagta gccgcactgc
     3001 cccgcctgct catcgaggaa cgcctgctgc aacggatgcg gtgccgccgg agtgcctagc
     3061 ccctcgatcg tcgtaacgcg gccgccggct gcggtgccaa cctcgcgcga gcaggcatgc
     3121 accagctcgc cgtccagcag caccacgcag ctgccgcatt gctccagtcc gcagccgtag
     3181 cgcgtgccca acagtcccag ctggttgcgc aacacatcca gcaaaggcgt cgtaccggcc
     3241 atagcagtct gcacaggccg gccgttgaca gtaagctcga tcatgccgtg cggcgctgta
     3301 cgttcgcgct cctcaccggg atgccggtct gctcgtaaca gacctgggcc agcgcggcga
     3361 ccccttcgcg gatcacctct ttcgacggca gcgcgaaaca caggcgcata tacgacttgg
     3421 caggctccgg gttgcatacc cactcggggc ccggattgaa ctggatgccg gcatccgccg
     3481 ccggcttcac aagcttccgt acatcgacgc tgtccggcag cttgaaccac aggaagatgc
     3541 cgcccttcgg cttccacatc tcggcgctgg tgccgaactc gcgttccacc gcctcgatca
     3601 tcgtgtcgag cttctccttc agcacgttgg tcagcgcgcc gacatgccgg tcgaaatgat
     3661 tggcgaagta ttccgccaca atcatttgat ccagcgcacc ggtgccgctg tcggtcttgc
     3721 acgccagcat acgcgacagc accggccagt cggacactgc gtagccaacg cgcaaggccg
     3781 gcgccaatgt tttg
//
LOCUS       DATGEM010000429         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_597350, whole genome shotgun sequence.
ACCESSION   DATGEM010000429 DATGEM010000000
VERSION     DATGEM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597350"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..409)
                     /gene="hfq"
                     /locus_tag="VKQ27_13750"
     CDS             complement(122..409)
                     /gene="hfq"
                     /locus_tag="VKQ27_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HLY90041.1"
                     /translation="MANEKAQNVQDVFLNHVRKQKTPVTVFLVNGVKLQGIITWFDNF
                     SVLLRRDGHTQLVYKHAISTVMPGAPIQLFDASKVEGAAAPVREMTSAASE"
     gene            complement(491..1147)
                     /locus_tag="VKQ27_13755"
     CDS             complement(491..1147)
                     /locus_tag="VKQ27_13755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLY90042.1"
                     /translation="MRTPRILLYDWDNTLVDGWAGITGALNATFSAFAMPLWSVEDTR
                     ARVRVSLRDSFPAMFGDKWERARDIFYGALEAQHLQHVRPMPGAGDALLAGRPWPQGV
                     VSNKAGHYLRAEVVHLGWADHFGAVVGAGDASADKPDPAPIFLALDRMGASADRSVWY
                     LGDTALDMAAARAAGVTAVLVGDGAHDGGIDQSHYDLHFPSALDLAARLRALARDGID
                     "
     gene            complement(1144..2397)
                     /locus_tag="VKQ27_13760"
     CDS             complement(1144..2397)
                     /locus_tag="VKQ27_13760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90043.1"
                     /translation="MPPSRPYLDMITRTLGVNANTVLHQGQACKLCGGFSPPFDSLDF
                     FKYCAPTNPFAFGFAGIHVEYRRCKDCGFIFTDFFDDWTPQEFATYVYNTDYARIDSE
                     YANIRPNNVAAQVAERLAAFRGIDILDYGSGSGAFSERMAAHGFANITNYDPYSSPAR
                     PEGSFSLITCFEVIEHAVSPRACLQDMASFLAPGGCIIFSQPLQPDDIETIRGNWWYI
                     GPRNGHASIFTADALARLAGECGLVFYRGDWLHGFAPANVTERSRDMLKAIGAPYASV
                     RLDAPLDSLANEAVRADQRDSWHSLERGGGFRFRWTRQDRIAWHGVLPGVYPVRARIA
                     VPFHNQIRDGFAAACTIGVGDQVLPVKVQGTELIVETTIERPVENIVTLHTPEPASPR
                     SLRGVNDDRPLGLAIAAYPWEPPAL"
BASE COUNT          452 a          865 c          830 g          461 t
ORIGIN      
        1 cgaacggcgc gcgtcctgcg gacgccccgg ttcgtggtca ctacgatccc atggcagcac
       61 caccgctgcc cgtgtgggca gctggccagc gccgttcatc cggtcggggg agttacgccc
      121 atcactcgct tgcggcagat gtcatctccc gcaccggcgc cgccgcaccc tccactttgc
      181 tcgcgtcgaa caactggatc ggggctcccg gcatcacggt gctgatcgcg tgcttgtaaa
      241 caagctgcgt gtgcccgtcc cgtcgcagca ggaccgaaaa gttgtcaaac caggtgatta
      301 tcccctgcag cttgaccccg ttcaccagga acaccgtcac tggcgtcttt tgcttccgca
      361 cgtggttcag gaacacgtcc tgaacgttct gcgccttctc gttggccacg gttgttatcc
      421 ttcttgttgg ctagccgttg ttggttgttc cgccggtgcg ggcgtggcac cgccgccccg
      481 gtgtagtcct tcagtcaatc ccatcccgcg caagcgcacg aagacgtgcg gcaaggtcga
      541 gcgcggaagg gaaatggaga tcataatgcg attggtcaat cccaccgtca tgcgccccgt
      601 caccgaccaa taccgcggtg acgccggccg cgcgcgccgc cgccatgtcg agcgcggtat
      661 cgcccagata ccagaccgac cgatcggctg acgcccccat ccgatcgagc gccaggaata
      721 tcggcgccgg gtctggcttg tcggcgcttg cgtcgcccgc cccgaccacc gcgccgaaat
      781 ggtcggccca gccgagatgc acgacctcgg cccgcagata gtgcccggcc ttgttcgaca
      841 ccacgccctg aggccacggc ctccccgcca gcagcgcatc ccccgcgccc ggcatcggtc
      901 gcacgtgctg cagatgctgc gcctccagcg cgccgtagaa gatgtcgcgc gcccgctccc
      961 acttgtcgcc gaacatcgcg gggaagctgt cgcgcagaga tacgcgtacc cgcgcgcgcg
     1021 tgtcctccac gctccacagc ggcatggcga aggcgctgaa cgtcgcgttg agtgccccgg
     1081 tgatgccggc ccagccgtcc accagcgtgt tgtcccagtc gtagagcaat atccgcggcg
     1141 ttctcatagg gcgggcggtt cccaaggata ggcagcgatc gccagcccca gcggtctgtc
     1201 gtcgttcacg cctcgcaggg agcgcggaga tgccggctcc ggtgtatgca gcgtcacgat
     1261 gttctccact ggccgctcga tcgtcgtttc cacgatcagt tccgtgcctt gcaccttcac
     1321 cggcagcacc tggtccccca cgccaatcgt gcacgcggcg gcaaagccat cgcggatctg
     1381 gttatggaac ggcaccgcta tgcgcgcccg caccggatag acccctggca gcacgccatg
     1441 ccaggcaatc cggtcttgcc gggtccatcg gaaccggaag cccccgccac gttccagact
     1501 gtgccagctg tcgcgttgat ccgcgcgcac cgcttcattc gcgagactgt ccagcggcgc
     1561 gtcgaggcga accgacgcgt acggtgcacc gatcgccttc agcatgtcgc gtgaccgctc
     1621 cgtgacattc gccggggcaa agccatgcag ccagtcgccg cggtagaaca ccagcccgca
     1681 ctcgccggca agtcgcgcga gcgcatctgc cgtgaagatc gacgcatgcc cgttgcgtgg
     1741 cccgatatac caccagttgc cgcggatggt ctcgatatcg tccggctgca acggctggga
     1801 aaagatgatg cagccgccgg gggccaggaa cgaggccatg tcctgaaggc aggcgcgcgg
     1861 cgaaaccgcg tgctcgatga cctcgaagca ggtgatcagg ctgaagctgc cctctggccg
     1921 cgccgggctc gaataggggt cgtagttggt gatgttggcg aagccatgag cggccatgcg
     1981 ctcggagaag gcgcccgagc ccgatccgta atccaggatg tcgatgcccc tgaatgcagc
     2041 gagccgttcg gcgacctggg cggcgacatt gttcgggcgg atattggcgt attccgagtc
     2101 gatccgggcg tagtctgtat tgtagacgta ggtggcgaac tcctgcggcg tccagtcgtc
     2161 gaagaagtcg gtgaagatga agccgcagtc cttgcagcgg cggtattcca catggatgcc
     2221 ggcgaacccg aaagcaaacg gattggtggg cgcacagtac ttgaagaagt cgaggctatc
     2281 gaacggcggc gagaacccgc cgcacagctt gcaggcctgg ccctggtgga gaacggtgtt
     2341 cgcgttcacc cccaacgtgc gggtgatcat gtcgaggtag gggcgcgagg ggggcatgac
     2401 ggtctctggc acctggtgac ataccggtcg cccggcgact gcaccttact ctgtaatgca
     2461 agagcggcaa tgcaagagcg acgcgttcgt cgggatccct tctggttcag cggcagccac
     2521 ccggcacagt ggtctgcgca cagtaccagt ttaacaagta ggtgagagta gtcgggctgg
     2581 actctggcaa gatcttgctt gggttggg
//
LOCUS       DATGEM010000430         5809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1398277, whole genome shotgun sequence.
ACCESSION   DATGEM010000430 DATGEM010000000
VERSION     DATGEM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398277"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..3058)
                     /locus_tag="VKQ27_13765"
     CDS             complement(113..3058)
                     /locus_tag="VKQ27_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLY90044.1"
                     /translation="MAVATRPLPDVQRGYYWLIGIASLVLLAVLAIWQVPQWLDWTRY
                     RGTIEVLATSMLGRPVAIHGPIALTLLPEPLLTATQVDIGAGSTADISVHVKALRLRV
                     ALWPLLAGRVEARNLVLRGADVHVPWPAESRVLRAHPPAWLGAFSARIEEGSFSIGRI
                     ALSGIDATLTSSETGAFSAAGSAKFNGQDWSFSARMTAPDEDHVVGLDATLDAQGKAA
                     GLGATVTGQLSAEGGFSGSISSHGPDLSMLVPAPQVPFRADGRLTIAGGLAIADNLVL
                     QIGGSLAAGAVALRVSPVQRLDIALSASRLDLDTWLPVLLGARNSVAGIDLPIGVDLS
                     AKAAPLGGDLLENVRAAFELSDQALTLREGSTLLPGNGKLWLSGVMKHSDATRPEFVG
                     DARLDAPVLRTTLQWLHKAAPGWLPDAALAHLPDGVLQHAILAAHVSASHDAVLLRTV
                     TGDVDASSIAGSLGFRRGAVPAFSADLTVDHASLDDWTPVSTRVPQSVDADVRLNIHA
                     AALHGYTLNDLALDGSVTGGVVTLRQLAATLTGLQISASGKLGKDQQVSDVKLNLVTQ
                     DATPLAAMLPGIWQATPALWHGPLRLSLQAAGPPNALGVTAQLTLDDATAEATPTIDL
                     ASGAWNGELTVRHPAARRLIAALALPDLTGLSELPDWLGDGSLSLVAHLHGAPNKIAA
                     DSLDMTAGGLRFGGDFMLDQSGGEPHLHGQLHADNVPVPVPSDASSVPLPIGLLRGWQ
                     ADLTLTAGRLMFGDEAVRDVSASISVADGSLRIERLTGRLGGGTLSGICLFNGLASPP
                     SLAAQVRIDGATVAEPLGIAPIDLVDGDVQGSIDLTASGYSPAALVSTLAGHLSLTVT
                     DGAVAGFDLSGARRAAQQTDAVAAQRTVTDALDGGTTRFDNLQLAGTLTHGSLSLDTV
                     RLSGGAGEVDGGGDANLPDKELNLHIVLRPAMQNPPEIGVQLTGPFAHPQRTLDLANL
                     ARFVAERAH"
     gene            complement(3030..>5809)
                     /locus_tag="VKQ27_13770"
     CDS             complement(3030..>5809)
                     /locus_tag="VKQ27_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY90045.1"
                     /translation="IDHIIGFDMGGTSTDVALYAGGFERAFETAVAGVRMRAPMMAIN
                     TVAAGGGSILHFDGARFRVGPDSAGAEPGPACYRNGGPLTVTDANVCVGKIQPRHFPA
                     IFGQNGDQPLDLDVVTQKFAALAEEITQATGQHRTPQQVAEGYLQIAVANMANAIKQV
                     SVQKGHDATRFALQCFGGAGGQHACLVADALGMETVFIHPFAGVLSAYGMGLADQTVM
                     REQAVEIPLEASALPKLTELADRLAAETTEALITQGAEASKVTTRRSLHLRYSGTEAA
                     LIVALRTPPEIIEDFTAAHRARFGFATPERPLVVEAVAVEAVSPGDVVREATLAARTS
                     GAPSTIDTIQIFTGDSEHTAPVFDRTDLLAGDRIKGPALIREANATTVVEPGWTAEIT
                     ALDHMTLRRTERLATRAAADSGRPDPVLLELFNNLFMNVAEQTGAVLQNTSMSVNIKE
                     RLDFSCAIFDAQGFLVANAPHVPVHLGAMGESVRTVLNNRRTTLKRGDVVVLNNPFNG
                     GTHLPDVTVITPVFDTDGRDILFFVGSRGHHADIGGITPGSTPPISRTLEEEGVVLDD
                     FLLVDGGHFREGEFRALLTGAKYPARSPDVNVADVKAQVAANEKGVQELQRVVALYGW
                     PTVSAYMRHVMDNAEEAVRRVIDRIGSGSFEYTMDSGLKLCVSISIDHGSRSAVVDFA
                     GTGHQSEGNFNSPPAVTRAAVLYVFRTLVADDIPLNDGCLKPLKLIIPPGTFLSPKPG
                     AAVVAGNTEVSQATCNALFGALKPIACSQATMNNFLFGDATRQYYETICGGTGAGPGF
                     DGTSAIHSHMTNTRMTDPEVLELRYPVRLERFAIRRGSGGKGKWHGGDGSIRRIRFLE
                     PMKAVIVSSRRDVAPFGLEGGEDGSVGRQWVERADGMQQVLTGTDSADMNPGDVFVIE
                     TPGGGGYEAAA"
BASE COUNT         1066 a         1965 c         1764 g         1014 t
ORIGIN      
        1 tgcgccagga gccgcttcaa agccgatgag gagactgcca taaccggccg gtcgagccag
       61 actcggttta ctgcagcaat cgatgggtga ctcgatcgaa gcccatcccg ctttaatgcg
      121 ctcgctcggc cacgaagcgc gccagattgg ccagatccag tgtccgctga ggatgagcga
      181 atgggccagt cagctgcacg ccaatctcag gcgggttctg catggccgga cgcagcacga
      241 tatgcaggtt cagttccttg tcaggcaggt ttgcatcacc gccgccgtcg acctccccgg
      301 cgccgcccga cagccgcacg gtgtccagcg acaggctgcc atgcgtgagg gttccggcaa
      361 gctgcaggtt gtcgaaccgc gtcgttccgc catccagggc gtcggtgacg gtgcgttgtg
      421 cggccacagc gtctgtctgc tgcgcggcac gccgcgctcc tgacagatcg aaaccggcaa
      481 ccgcgccgtc ggtgacggtc agcgacaggt ggcctgcaag cgtagataca agtgccgcgg
      541 ggctgtagcc gctcgcggtc agatcgatgc tgccctggac gtcgccgtcg accaggtcga
      601 ttggcgcaat gcccaacggt tcggcgaccg tcgcaccatc aatccgcacc tgtgctgcca
      661 ggcttggcgg gctggccagg ccgttgaaca gacatatgcc actcagcgtc cctccgccga
      721 gcctgccggt gaggcgttcg atccgcagtg agccatcggc gacactgatc gaggccgaca
      781 catcgcgtac agcctcatcg ccgaacatca agcgtccagc cgtcaacgtc agatcagcct
      841 gccagccgcg cagcaagcca attggcagcg gcacagacga tgcgtcgctg ggcactggaa
      901 ccggcacatt gtcggcatga agttgcccgt gcagatgcgg ttcaccgccg gactgatcga
      961 gcatgaagtc gccgccgaac cgcaggcctc ccgcggtcat atcgaggcta tcagcggcga
     1021 tcttattggg cgcgccatgc agatgcgcga ccagtgacag cgagccatca cccaaccagt
     1081 cagggagctc cgataatcca gtcaggtccg gcagcgccag cgcggcgatc agccgccgcg
     1141 cggccggatg ccgcactgtc agctcaccgt tccatgcgcc cgacgccaga tcgatcgttg
     1201 gtgtcgcctc cgccgtagcg tcgtccaacg tcagctgcgc cgtgacgccc agtgcgttgg
     1261 gcggccccgc tgcctgtagt gacaatcgta gtggtccatg ccacaacgcg ggcgtcgctt
     1321 gccagatccc aggcagcatg gccgccagcg gcgtcgcgtc ctgcgtcacc aggttgagct
     1381 ttacgtcgct gacctgctga tccttcccaa gcttgcctga ggcagaaatc tgcagcccgg
     1441 tcagggtcgc tgcgagctgc cgcaatgtca ccacgccgcc cgtcacgctg ccatccagcg
     1501 ccagatcatt caacgtatag ccatgcagcg ccgccgcgtg gatgttgagg cggacatcgg
     1561 catcgacaga ttgcggcacc cgtgtcgaaa ccggggtcca atcatccagc gacgcatgat
     1621 ccaccgtgag gtcggctgaa aacgccggca cggctccccg gcgaaaacca aggctgccag
     1681 cgatcgagga tgcatccacg tcgccggtga cagtccgcag cagcaccgca tcgtgacttg
     1741 ccgaaacgtg ggcggcgagt atggcatgtt gcaacacgcc atccggaaga tgcgccagcg
     1801 cagcgtccgg caaccaacca ggtgctgcct tatgcagcca ttgcagcgtg gtgcgcagaa
     1861 ccggcgcatc caggcgggcg tctcccacga attccggtcg cgttgcatcg ctatgcttca
     1921 tcacaccgga caaccacagc ttgccattgc caggcagcaa tgtgcttccc tcgcgcaggg
     1981 tcagcgcctg atccgaaagc tcgaacgctg cgcgtacgtt ctccagaagg tcgccgccca
     2041 gcggagcggc ctttgccgac aggtccacgc caatcggcag atcgatcccg gctacggaat
     2101 tgcgtgcgcc gagcaacacc ggcagccagg tatccaggtc gaggcggctg gccgacaacg
     2161 caatgtccag ccgctgcacc ggtgacacgc gcaacgcgac ggcaccggcc gcgagtgagc
     2221 cgcctatctg cagcacaagg ttgtcggcga tcgccaatcc gccggcgatg gtcaatcgtc
     2281 catctgcgcg aaatggtacc tgtggcgcag gcaccagcat cgagagatcg ggaccatggc
     2341 tgctgatgct gccgctgaaa ccgccttccg cactcaattg tccagtcacg gtggccccga
     2401 gcccagcggc ttttccttgc gcgtcgagag ttgcatccag tccgacaacg tgatcctcgt
     2461 cgggcgccgt cattcgtgca gaaaaactcc agtcctgacc attgaacttt gctgaaccgg
     2521 cagcggagaa tgcgccggtt tcgctcgaag tcagcgttgc atcgatgccg ctgagagcaa
     2581 tgcgtccgat gctgaaactg ccttcttcga tacgtgctga aaaagcacca agccaggctg
     2641 gcggatgcgc acgcagcacg cgtgactcgg cgggccaggg aacatgcacg tcagcgccac
     2701 gcagcaccag gttgcgcgct tcgacacgac cggcgagcaa tggccacagc gctacgcgca
     2761 accgcagcgc tttcacatga accgagatat ccgcggtgct gcctgcgccg atatcgacct
     2821 gtgtcgcggt caacagcggc tcgggcaaca aggtcagcgc aatcggaccg tggatggcca
     2881 caggccggcc gagcatcgag gtcgccagca cctcgatcgt gccgcgatag cgcgtccagt
     2941 cgagccattg cggtacctgc cagatcgcca gcacggcaag cagcaccagg ctggcaattc
     3001 cgatcagcca gtagtacccg cgttgcacgt caggcagcgg cctcgtagcc accgccaccg
     3061 ggcgtttcga tcacgaatac atcgccagga ttcatgtcgg cgctgtcggt gccggtcagc
     3121 acctgctgca tgccatcggc gcgctccacc cattgccggc cgacggagcc gtcttcgccg
     3181 ccttccagac cgaagggcgc gacatcgcgt cgcgacgaga cgatcaccgc cttcatcggc
     3241 tccaggaacc ggatgcggcg gatcgagccg tcgccgccat gccatttgcc cttgccgccc
     3301 gatccacgcc ggatcgcaaa gcgctccagc cgcacagggt agcgcagttc cagcacttcc
     3361 ggatcggtca tgcgcgtatt cgtcatgtgc gagtgtatcg ccgaggttcc atcgaacccc
     3421 ggtcccgcac cggtaccgcc acagatcgtc tcgtagtatt gccgcgtggc gtcgccgaac
     3481 aggaagttgt tcattgtcgc ctgcgaacag gcgatcggct tcagtgcgcc gaacagcgcg
     3541 ttgcacgtcg cctgtgagac ttcggtattc cctgctacca ccgcggcacc cggcttcggc
     3601 gagaggaacg tgcccggcgg gatgatcagt ttcagcggct tgaggcagcc gtcgttcagc
     3661 ggaatgtcgt ccgcgaccag ggtgcggaac acatacagca ccgcggcacg tgtcaccgca
     3721 ggcggcgagt tgaaattgcc ctcgctctgg tggccggtgc cggcgaaatc caccaccgcg
     3781 ctgcggcttc catgatcgat gctgatcgac acgcacagct ttaacccgct gtccatcgtg
     3841 tattcgaacg agccgctgcc gatgcgatcg atcacccgcc gcacggcttc ctcggcgttg
     3901 tccatcacgt ggcgcatata cgcgctgacc gtcggccagc catacagtgc cacgacgcgt
     3961 tgcagttcct gcaccccctt ttcgttcgcc gccacctgcg cctttacgtc ggcgacattc
     4021 acatccggac tgcgcgcggg atatttcgca ccggtcagca aggcgcggaa ttcgccctcg
     4081 cggaaatgtc cgccatcgac cagcaggaag tcgtcgagca ccacgccttc ctcttccaga
     4141 gtgcgggaga ttggcggcgt ggacccgggc gtgatgccac cgatatccgc gtgatgtccg
     4201 cgactgccga cgaagaacag gatgtcgcgg ccatcggtat cgaacaccgg cgttatcacg
     4261 gtgacgtcgg gcagatgcgt gccgccgttg aacggattgt tcagcaccac gacgtcgccc
     4321 cgcttcagcg tggtgcggcg gttgttcaac accgtgcgca cgctttcgcc catcgcgccg
     4381 agatgcacgg gcacatgcgg tgcattcgcc accaggaagc cctgtgcatc gaatatcgcg
     4441 caggaaaaat caagccgctc cttgatgttg acgctcatcg aggtgttctg cagcaccgcg
     4501 ccggtctgtt cggccacgtt catgaacagg ttgttgaaca gctccagcag cacgggatcc
     4561 ggcctcccgc tgtcggcggc ggcgcgcgtc gccagccgct cggtacggcg cagcgtcata
     4621 tgatccagcg cggtgatttc ggcggtccag ccgggctcca cgactgtcgt cgcgttggct
     4681 tcacggatca gggctgggcc tttgatgcga tcgcctgcca acaggtcagt gcgatcgaac
     4741 actggagcgg tgtgctcact gtcgccggtg aatatctgga tcgtatcgat tgtcgatggc
     4801 gctccgctgg tgcgcgcagc cagtgtcgcc tcgcgtacca catcaccagg cgaaaccgcc
     4861 tcgaccgcga cagcctccac caccagcggc cgttccggcg tcgcgaaccc gaagcgtgcg
     4921 cgatgcgcgg cggtgaagtc ctcgataatc tcgggcggcg tccgtaacgc cacgatcagc
     4981 gccgcttccg tgcctgaata gcgcaaatgc agactgcgac gcgtcgtgac cttcgacgct
     5041 tccgcgccct gcgtgatcag tgcctcggtc gtttccgcgg ccagccgatc ggcaagctcc
     5101 gtcagcttcg gcagggccga ggcctcgagg ggaatttcca ccgcctgttc gcgcatgacc
     5161 gtctggtctg caagtcccat gccatatgcc gagagtacgc cggcaaacgg atggatgaag
     5221 actgtctcca tgcccagggc atctgcaacc agacacgcat gctgtccacc agcgccaccg
     5281 aagcattgca acgcgaagcg tgtcgcatcg tgccccttct gcacggatac ctgcttgatc
     5341 gcattcgcca tgttggcgac ggcaatttgc agataaccct cggccacctg ttgcggcgtg
     5401 cggtgctgac ctgtcgcctg agtgatctcc tcggccaggg ctgcgaattt ctgcgtcact
     5461 acatccagat caagcggctg atcgccattc tgcccaaaga tcgcagggaa gtgccggggc
     5521 tggatcttgc cgacacagac attcgcgtcg gtgaccgtca gcggaccacc attgcggtaa
     5581 cacgctggtc ccggttcggc acccgcactg tccggcccca cccggaaccg cgcgccatca
     5641 aaatgcagga tggagccgcc gcctgcggcc accgtgttga ttgccatcat cggtgcgcgc
     5701 atgcgcacgc cggccactgc cgtctcgaac gcgcgctcga acccgccggc atagagcgcg
     5761 acatctgtcg aagtgccgcc catatcgaag ccgatgatgt ggtcgatcc
//
LOCUS       DATGEM010000431         7511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_356965, whole genome shotgun sequence.
ACCESSION   DATGEM010000431 DATGEM010000000
VERSION     DATGEM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356965"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VKQ27_13775"
     CDS             complement(<1..851)
                     /locus_tag="VKQ27_13775"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HLY90046.1"
                     /translation="MSEAIATDALSLHRGLLTLDTHIDIPWPTGPDPFLEGTRRVDLP
                     KMQRGGLAAGCFVAYVPQALRTPETDAAAYERVIGMLQTIRGMGHTEHGITARITATV
                     AEIEAAKRDNVLAIVPAVENGFAMGTDLSRLARFRALGARYMTLTHTGHNAIADSANP
                     RADLNNAPTEHGGLSELGRAAVAELNRLGMLVDVAHVSRDTMLQAAAISRTPVVSTHS
                     CIRALCDHPRNMDDTQLDALRDVGGVIQITAVAAFLKKDAKPEAVTVRDFADHVDYAV
                     QRIGLQHV"
     gene            931..1614
                     /locus_tag="VKQ27_13780"
     CDS             931..1614
                     /locus_tag="VKQ27_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90047.1"
                     /translation="MVIPRVPLLVISALAIGGPACAQLRPASEAIGSWVLSCPDAKAV
                     PCRLRDRMPIVQASENGPAASLEVAHRGNEFVPVVALRGMSMQAAVGGMLAVQGSVTL
                     RFDNAPAIELGCGLDGSAVVCAPSGAAAAAAAAALPDAHNVVVRVRMTLPGAMAFPEQ
                     SRSLDLQETGQALAQFRATAPASESVPVIAGLDWRGFLDRMARDAGFRNGLADLLSTG
                     RTLIDGRPP"
     gene            1637..2104
                     /locus_tag="VKQ27_13785"
     CDS             1637..2104
                     /locus_tag="VKQ27_13785"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY90048.1"
                     /translation="MPDTLDGGCACGALRYRMHSTPMFVHCCHCKDCQRQTGTAFVLN
                     ALIEADRVELLSGDPQPSAMPTDSGKPHRVFRCPDCGTAVWSEYGGLEKLRFVRVGTL
                     DEPTALPPDVHIYIRSKLPWVTLPDGVPAFASYYSSREVWPAASLERRKAVMQ"
     gene            2439..4247
                     /locus_tag="VKQ27_13790"
     CDS             2439..4247
                     /locus_tag="VKQ27_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY90049.1"
                     /translation="MAPPLLLLQDISLSFGTSPVLAGAGLAVAAGDRLCLVGRNGSGK
                     STLLRIAADLIQPDAGRRFAQPGATIRYLPQEPDLSGFDNTLAYSQAGLVDDADEDRH
                     RARYLLEQLGLTGDEHPATLSGGEARRAALAQVLAPSPDILLLDEPTNHLDLPGIEWL
                     EQELASMRSGIVVISHDRRLLERISRATVWLDRGITRTLDQGFAGFEAWRDTVLEQEE
                     LERHKLTRKIAMEEDWLRYGVTARRKRNQKRLADLHALRKRRKEQRAAQGNVRLEAAQ
                     AELSGRLVAVAEHVEKSFGDRVVVRDFSTRVLRGDRVGIVGSNGAGKTTLLKMLTGEL
                     APDAGEVRLGTNLAQVTLDQRRETLDPMQTLVQALTGGEGDTVTVGGAKRHVIGYMKD
                     FLFTPEQANTPVGVLSGGERGRLTLARAFARPSNLLVLDEPTNDLDLETLDLLQERLA
                     EYSGTVLLVSHDRDFLDRVVTSVIAAEGNGRWIEYAGGYSDMLSQRGPVMAAAPQVAT
                     KVRAATESRPQRGSTPRRMTFKDRHALETLPARIAALQADVERLSAVLADADLYARDP
                     ARFSATTMALEAAQTALAAAEEQWLTLEMLREELEG"
     gene            4341..5168
                     /gene="panB"
                     /locus_tag="VKQ27_13795"
     CDS             4341..5168
                     /gene="panB"
                     /locus_tag="VKQ27_13795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HLY90050.1"
                     /translation="MPTKTITPTVIKARKGGPPIVCLTSYTAPMTKLVDPHADLILVG
                     DSLAMTIYGMASTVGVTQEMMIAHGRAVVHAASRACVVVDLPFGSYEESPMQAFRTAA
                     SIMAETGCAAVKLEGGTVMAETIHFLTSRGVPVMAHIGLTPQSVNALGGYAVRGRSES
                     EAAMIMADAEAVADAGAFSVVIEKTAEPLARAVTDRIIPPTIGIGASVACDGQILVVD
                     DMIGFFTEFHPKFVRRYAEVAQSVEAAIASYAADVRTRRFPAQEHVFAGTIRERPLA"
     gene            complement(5237..5761)
                     /locus_tag="VKQ27_13800"
     CDS             complement(5237..5761)
                     /locus_tag="VKQ27_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HLY90051.1"
                     /translation="MPFAVTLRLDEASAPAVEAMWRLLAEHGIDTDRHELGYPPHVTL
                     AIYADDVPVRRLHDALALCADRWKAVPISLSGLGVFPGLKSILWAAPVVSQQLLAVHM
                     ELQAVMPDLSVHAHYRAGAWVPHVTLSGPLADPARAIAALIPFWQPLTGSLARIDLVR
                     FRPIELMASIPLST"
     gene            complement(5904..7367)
                     /locus_tag="VKQ27_13805"
     CDS             complement(5904..7367)
                     /locus_tag="VKQ27_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY90052.1"
                     /translation="MQHHWDVEADVVVVGFGAAGAAAAVTAHELGASVVLLEKAPEGE
                     HGGNTRVAGQGYLNTSSIEKAVAYLTALCGPFTVPEKMVRVWAEEMGQNNAWLTSLGG
                     DPQEHQHQPVGIEFPDLPGADCVHKFHDGPTYGYSYTWQLFERLVLARPIKVLYESPA
                     RSLIQHDTAKEILGVRAQQDGRAIAVKARKGVVLTCGGFENNQEMIRNYLPGVPYCYT
                     SGSPYNEGDGITMAMSVGADLWHMNNYAGPSMALKVPDVRTSFSMQALHFSKQMPGGM
                     IVVGPDGRRFTDEKFKTRHGKVPVNGRWMPLAAPCPMFMIFDHTLFRAGPLYDTHPSH
                     GWTQIIERYEWSEDNSAELAKGWIKQADSIAALASMVGLDPAALGQTVRQWNASCEME
                     RDAEFGRRLMLAPIVEGPFYAVELSPSMLNTQGGPRRNEKAQIVRPDGSPIPRLYSAG
                     ELGSIYSYLYQGTGNIGECLAFGRIAARGAVAERPWS"
BASE COUNT         1296 a         2334 c         2513 g         1368 t
ORIGIN      
        1 acatgctgca ggccgatgcg ttgcaccgca taatcaacgt gatcggcgaa gtcgcgcacc
       61 gttacggcct cgggcttcgc gtcctttttc aggaacgcag caacggcggt gatctggatg
      121 acgccgccga cgtcgcgcag ggcatccaac tgcgtatcgt ccatgttgcg gggatgatcg
      181 cagagcgcgc ggatgcagga atgcgttgaa actaccggtg tgcgcgatat ggctgcggcc
      241 tgcagcatcg tgtcgcggga gacatgtgcg acgtcgacca gcatgccgag gcgattgagt
      301 tccgccacgg cggcacggcc gagctcgctc aagccgccat gttccgttgg cgcgttgttc
      361 agatccgcgc gtggattggc ggaatcggcg atcgcattgt ggcccgtatg agtgagggtc
      421 atgtagcggg cacccagcgc acgaaaccgg gcgaggcgcg acagatcggt gcccatcgcg
      481 aagccattct ccacagccgg cacaatggcg agcacgttgt cgcgcttagc cgcttcgatt
      541 tcggcgacgg ttgcggttat gcgagcagtg attccatgct cggtgtgtcc catgccacgg
      601 atcgtctgca gcatgccgat gacgcgctca tatgccgccg cgtcggtttc cggagtccgc
      661 aacgcctgtg gcacataggc cacgaaacag ccggcggcca agccaccgcg ctgcatcttc
      721 ggcagatcca ccctccgggt cccttccagg aagggatcgg gcccggttgg ccaggggatg
      781 tcgatatgcg tatcgagggt caaaagcccc cggtgcaagg agagggcgtc ggtcgcaatg
      841 gcttcactca tgatgccgaa cggtcgcaga tatcggcgcg acacggaaga ggcgggtacc
      901 tacaagtcgg cacgttgtcg agcgagattc atggttattc cgcgcgtgcc gctgctggtc
      961 atttccgccc tggcgatcgg cggtccggca tgcgcgcaat tgcgacctgc ttcggaggcg
     1021 atcggctcgt gggtgctgag ctgccccgat gcgaaggctg tgccgtgccg gttgcgtgac
     1081 cgcatgccga tcgtccaggc aagcgagaat ggaccggcag catcgctgga ggtggcgcat
     1141 cgcgggaatg agttcgtgcc ggtcgtggcg ctgcgcggga tgtcgatgca ggccgctgtg
     1201 ggaggcatgc tggccgtgca gggcagcgtt acgttgcgct tcgacaatgc accggcaatc
     1261 gagctgggct gcggattgga tggcagcgct gttgtttgcg cgccatcggg cgccgctgct
     1321 gcggcagccg ccgccgcatt gcctgacgcg cacaacgtgg ttgtgcgggt gcggatgacc
     1381 ttgcccggcg cgatggcctt cccggagcag tcgcggtcgc tcgatctgca agagaccggg
     1441 caggctttgg cacagtttcg tgctaccgct cccgcgagcg agtcggttcc ggtcatcgcc
     1501 ggcctcgact ggcgcgggtt cctggatcgc atggcgcggg atgccggatt ccgcaacgga
     1561 ctggccgatc tgctgtcgac cggcaggaca ctgatcgacg ggcggccgcc ataaaaggtg
     1621 gcgagggagg gatactatgc ctgatacgct ggacggcggc tgcgcctgcg gagcgctccg
     1681 ctatcgcatg cattccacgc cgatgttcgt gcattgctgc cactgcaagg actgtcagcg
     1741 gcagaccggc accgcgttcg tactcaatgc gctgatagag gcggaccggg tcgaactgct
     1801 gtccggcgat ccacagcctt ctgctatgcc gacggatagc ggcaagccgc accgggtctt
     1861 ccgctgtccc gattgcggca ctgctgtgtg gagcgaatat ggcggccttg agaaactacg
     1921 cttcgtgcgc gtcggcacat tggatgaacc gacggcgctg ccaccagacg tgcatatcta
     1981 catccgatcg aagctgccat gggtgacact gcccgacggt gtgccggcct tcgcgtctta
     2041 ctacagctca agggaggttt ggcctgcggc gagtcttgaa cgacgcaagg cagtgatgca
     2101 gtagggtgcc ggtgtcattc cgagcagagc gaagcggtct cggacaggat tgcttcgctg
     2161 cgctcacaat gacatgtggt gtgctgctct ggtctgcctt cgcgcccctg gaggttccgg
     2221 cgcgcttcgg cagcgcggtt ttcgcggagg tcgtcggctc cgtcttcaca gcggcagccg
     2281 gttccgccac gcgcagtact ggccattgga agccatgggt caagcggtag gtcccctcac
     2341 ccgcgaaggg ggcaggggat gcggatgcgt tcgctgtcct tagtccccaa ccctccccag
     2401 cttcgcggga gaggccgatt caggctatgc cgccgcccat ggcgccacct ctgcttctgc
     2461 tccaggacat cagccttagc ttcgggacct cgccggtact ggccggggcc gggttggccg
     2521 tcgcggcggg cgaccggctg tgcctggttg gccgcaatgg ctccggcaag tccaccctgc
     2581 tgcggatcgc ggccgacctg atccaaccgg atgccggacg ccgcttcgcc cagcccggcg
     2641 ccacgatccg ctatctgcca caggaacctg acctgtcggg cttcgacaac acgctggctt
     2701 attcacaggc cggtttggtc gacgatgccg atgaggaccg ccaccgggcc cgctatctgc
     2761 tggagcagct tggtctgacg ggtgacgagc atccggccac gctgtccggc ggggaggctc
     2821 gtcgcgcggc gctcgcccag gtgctggccc cctcgccgga tatcctgctg cttgacgaac
     2881 cgaccaacca tctcgacctg ccggggatcg agtggctgga gcaggaactg gccagcatgc
     2941 ggtccggcat cgtggtgatc agccacgacc gccgtctgct ggagcgcatc tcacgcgcta
     3001 cggtgtggct ggatcgcggg atcacgcgca cgctggatca gggtttcgcc gggttcgagg
     3061 cctggcgcga tacggtgctg gagcaggagg agctcgaacg ccacaagcta acgcgcaaga
     3121 tcgcgatgga ggaggactgg ctgcgctacg gcgtcaccgc gcggcgcaag cgcaaccaga
     3181 agcggctggc cgatctgcat gctttgcgca aacgccggaa ggagcagcgc gcggcgcagg
     3241 gcaatgtccg gctggaagcg gcgcaagccg agctgtcggg gcggctcgtc gcggtggcgg
     3301 agcatgtgga gaaatcgttc ggcgatcgcg tggtcgtacg ggatttttcc acgcgagtcc
     3361 tgcgcggcga tcgtgtcgga atcgttggtt cgaatggcgc cggcaagacg acattgctga
     3421 agatgctgac cggagagctg gcgccggatg cgggcgaggt gcggctcggg acgaatctgg
     3481 cgcaggtcac gctggaccag cgccgcgaga cgctcgatcc gatgcagacg ctggttcagg
     3541 cgctgactgg aggggaaggg gatacagtga cggttggtgg cgcgaagcgc catgtgatcg
     3601 gctacatgaa ggactttctc ttcacgccgg agcaggcgaa tacgccggtt ggggtgctgt
     3661 ccggcggcga gcgcggacgg ctgacgctgg cgcgcgcatt tgcccggcca tcgaatctgc
     3721 ttgtgctgga tgagccgacg aacgatctag atctggagac gctcgatctg ctgcaggaac
     3781 ggcttgccga gtattccggc acggtactgc tggtcagcca tgaccgcgat ttcctggatc
     3841 gtgtggtgac gtcggtgatt gccgccgagg gcaatggacg ctggatcgaa tatgccggcg
     3901 gctattccga tatgctgtcg caacgtgggc cggtgatggc tgcggcgccg caggttgcga
     3961 ccaaggtgcg ggctgcgacc gagagcaggc cgcagcgtgg ttcgacgccg cgccgcatga
     4021 ccttcaagga ccggcacgca ttggagacgc tgcctgcccg gatcgccgcg ttgcaggcgg
     4081 acgtcgagcg cttgagcgcc gtgctggccg atgcggatct gtatgcacgc gacccagcgc
     4141 ggttcagcgc gacgacgatg gcactggagg ctgcgcagac cgcgttggcc gctgcggaag
     4201 agcaatggct gacgctcgag atgctgcgcg aggaactcga agggtaacgc cgcgctactg
     4261 atcaccgagg cagtgcaggg gctgcatcat actctctgct tgccgaccaa cgatcccgag
     4321 gaaatgccgt gagcaagacc atgccgacca agaccatcac tccgaccgtc atcaaagccc
     4381 gcaagggcgg cccgccgatc gtctgtctca ccagctacac cgcgccgatg accaagctgg
     4441 tcgacccgca cgccgacctg atccttgtgg gcgactcact ggcaatgacg atctatggca
     4501 tggccagcac ggtcggggtg acacaggaga tgatgatcgc gcatggccgc gccgtcgtgc
     4561 acgccgcgtc ccgcgcctgt gtggtggtcg atttgccatt cggctcgtac gaggagtcgc
     4621 cgatgcaggc atttcgcacg gccgcgagta ttatggcgga gaccggatgc gccgccgtga
     4681 agctcgaggg cggcacggtc atggctgaga ctattcactt cctcacgagc cggggtgtgc
     4741 cggtgatggc acacattgga ctgacaccgc agtcggtgaa cgccctcgga ggctatgccg
     4801 tccgggggcg tagtgaatcc gaagcagcga tgatcatggc ggatgccgag gcggtcgcag
     4861 acgccggcgc gttcagcgtg gtgatcgaga agacggctga accgctcgcc cgggctgtca
     4921 ctgatcggat tattccgccg acgataggga ttggtgcgtc tgtcgcctgc gacggacaga
     4981 tcctggtggt ggacgacatg atcggcttct tcaccgaatt tcatccaaag ttcgtgcggc
     5041 gctacgccga ggtggcgcag tcggtcgaag cggcgatcgc cagctacgct gcggacgtac
     5101 gcacgcgtcg gttccctgcg caggagcatg ttttcgctgg cacaattcgt gagcgaccgt
     5161 tggcatgacg cactgccgga cggacagaac aagactgatg ggagggacta ttgcgttggg
     5221 gcgccacagc gctgcgtcag gtcgagaggg gaatactcgc catcaactcg atcggccgga
     5281 aacggaccaa atcgattcgg gcgagactgc ccgtgagcgg ctgccaaaat gggatcaaag
     5341 ctgcaattgc tcgggcagga tcggcgagag gacccgagag cgtgacgtgc ggcacccagg
     5401 cgcctgcccg gtaatgcgca tgcactgaca agtccggcat cacggcctgc agctccatgt
     5461 gcactgcgag tagctgctgc gacacaaccg gggcagccca gaggatcgat ttcaagcccg
     5521 ggaacactcc aagacctgat agggatatgg gaacggcctt ccaccggtcc gcgcacaaag
     5581 caagggcatc gtgcaatcga cggaccggca catcgtccgc gtagatcgct agcgtgacat
     5641 gtggaggata gcccagctca tggcgatcgg tgtcaatgcc gtgctcggcg agaaggcgcc
     5701 acatcgcttc cactgcaggt gcgcttgcct cgtccaatcg aagggtgacc gcgaaaggca
     5761 tactcgtatg ctggcattga tccggctctc agtgaatgcg tggcaacagc aaaggcccca
     5821 cgaccttctc gccagcaact gcctgacaac tcgttgaccc cagcggtggg accaggcgtg
     5881 attcgaccaa atctcgccag actctagctc cacggccgct ccgccactgc acccctcgcc
     5941 gcgatgcgcc cgaatgccag gcattcgcct atattgccgg tcccctggta tagataactg
     6001 tatatcgacc ccagctcgcc agcgctgtac agccgtggaa tcggcgagcc gtcggggcgc
     6061 acgatctgcg ctttttcatt ccgccgcggg ccgccctggg tgttcagcat cgagggtgat
     6121 agctccaccg cgtagaatgg cccctcgacg atcggtgcca gcatcagcct gcgtccgaac
     6181 tccgcatcgc gctccatctc acaggatgcg ttccactgcc ggacggtttg tccgagcgcc
     6241 gcaggatcaa gcccaaccat cgaggccagt gcagcaatgc tgtcggcctg cttgatccag
     6301 cccttcgcaa gttccgcgct gttatcctcg ctccactcgt agcgttcaat gatctgcgtc
     6361 cagccgtggc ttgggtgcgt gtcgtacagc ggtccggcgc ggaacaacgt gtgatcgaag
     6421 atcatgaaca tcgggcatgg cgctgcgagt ggcatccagc ggccattcac gggcaccttg
     6481 ccatgccgcg tcttaaattt ttcatccgta aagcgccgtc catccggccc gaccacaatc
     6541 atgccgccgg gcatctgctt gctgaaatgc agcgcctgca tggaaaagct ggtgcgcacg
     6601 tccggcacct tcagcgccat cgatgggccg gcgtagttgt tcatgtgcca gagatcggca
     6661 ccgaccgaca tcgccatggt gatgccatcg ccctcgttgt acggcgagcc ggacgtgtag
     6721 cagtaaggca cgcccggcag ataattccgg atcatctcct gattgttctc gaaaccgccg
     6781 caggtcagca ccacgccctt gcgcgccttc accgcgatcg cgcggccgtc ctgctgcgcg
     6841 cgcacgccca ggatctcctt cgccgtgtcg tgctggatca gcgagcgggc tggtgattcg
     6901 tacagcacct tgattggcct cgcgagcacc agacgctcga acaactgcca ggtgtaggag
     6961 tagccgtagg tcggtccgtc gtggaacttg tgcacgcagt cggcgccggg caggtcgggg
     7021 aactcgatac cgacgggctg atgctggtgc tcctgcgggt caccgccgag gcttgtgagc
     7081 caggcattgt tctggcccat ctcctcggcc cacacccgca ccatcttttc cggcacggta
     7141 aacggcccgc acaacgcggt gagataggcg actgccttct cgatcgacga ggtattcaga
     7201 tagccctggc cggcgacgcg tgtgttgccg ccgtgctcgc cctcgggcgc cttttccagc
     7261 aggaccacgc tggcgcccag ttcgtgggcg gtgactgccg cggcggcgcc tgccgcgccg
     7321 aagccgacga ccacgacatc ggcctcgaca tcccagtgat gctgcatcga accttggtgc
     7381 tccgcgacgt gcttcggccg gcggagccgg tccccgatca gccgtccgga tgttgttctg
     7441 ttttgcaccg cggcgcaatt gcgggtgttt gccgcacagc ggtcacatcg ctatggtccg
     7501 ccccgcccac g
//
LOCUS       DATGEM010000432         8884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_864391, whole genome shotgun sequence.
ACCESSION   DATGEM010000432 DATGEM010000000
VERSION     DATGEM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864391"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VKQ27_13810"
     CDS             complement(<1..230)
                     /locus_tag="VKQ27_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HLY90053.1"
                     /translation="MDDVAVSFRGLPDEAPLAMPTQSLREAPARVRRPASAPRFMAVR
                     RLLVFGGAVALTGAGAAEMYQVFTVNGVTVLAV"
     gene            complement(218..1723)
                     /locus_tag="VKQ27_13815"
     CDS             complement(218..1723)
                     /locus_tag="VKQ27_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HLY90054.1"
                     /translation="MRRRELLLRSAALPIVAAIGGRAYAGETTSFDGSTVRNMAREIA
                     QKPYQAPDATLPDTLKNLDYQQYRSIRFDPAHALWKGEGTHFTAEFFHRGYLYKEAVQ
                     IYEVTNGRATHIRYNPSMFTFDKVTPPTADIGFAGFRLHYPMNRPDYFDEVSAFLGAS
                     YFRAVAKHQGYGLSARGLAIKTAEPSGEEFPLFKAFWLERPAKGADAVVIHALLDSQS
                     AAAAFRFTIRPGVQTVFDTEMALYPRVDIATAGIAPLTSMFMFDSNDRARADDYRDAV
                     HDSNGLLLWTGKGEHVWRPLANPRALQISAFTDSGTRGFGLMQRERQLSDFQDLEAHY
                     ENRPSAWIEPIGDWGQGIVELVEIPSDREVNDNMVAFWRPHDPLKAKGEYLLNYRLHW
                     CWTAPTPQSVAKVVATRSGLAWDQKNRLFVIDFAGGTLAERKPDALPTIDVGASKGKI
                     ENPVVEPNPDIKGMRLSFQLDQGSENLIEMHARLIDGETPLTETWIYRWTT"
     gene            complement(1878..3032)
                     /gene="grrM"
                     /locus_tag="VKQ27_13820"
     CDS             complement(1878..3032)
                     /gene="grrM"
                     /locus_tag="VKQ27_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophane-forming radical SAM/SPASM peptide
                     maturase GrrM/OscB"
                     /protein_id="HLY90055.1"
                     /translation="MGREAPRIDVVVVQPTPFCNIDCTYCYLPSRNDRTLIAQSTVRN
                     LFGKLFASGWANQSVNVIWHAGEPLVAPIAFYREAFGAIEKLRPREIDIMHSFQSNGM
                     LLTPAWCDLIEEWHVDIGISIDGPRHLHDRHRVTRSGQGTFDKTIAGVRLLRERRMPF
                     HTISVLSRDSLDAADEMYAFFVAEGIDHVCFNVEESEGQHTSDMLAVGDVRTRFRRFL
                     TRFWQLARASDKVKFVREVDTMINAVFRPNAAPFRNQQVEPLAMLNMDCLGNVSTFSP
                     ELLGYKQAEYNDFIIGNINTDSMEQIRDSRWLAAMQRDVDAGVEKCRANCEYFSICGG
                     GAPMNKLAENGSFDSTKTVFCSLIQMAAADVVLASARALQRTWQPEAATA"
     gene            complement(3035..3529)
                     /gene="grrA"
                     /locus_tag="VKQ27_13825"
     CDS             complement(3035..3529)
                     /gene="grrA"
                     /locus_tag="VKQ27_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrrA/OscA1 family cyclophane-containing
                     rSAM-modified RiPP"
                     /protein_id="HLY90056.1"
                     /translation="MSRHDRYLRFLTALLPAGAFGLSVALASSAAKATPVEPQQPRTE
                     PDIDTFSVANELQAIRDAVDEASAGILGGHVGQIPDPDIRLAWWLNGNGLGWGNGGHV
                     WGNGGYPAWGNGRPWGNGWGGWVNSAPWGNGWRNGWGNGGWHNGGWRNGGPWGNWHNG
                     GWRN"
     gene            complement(3619..4197)
                     /locus_tag="VKQ27_13830"
     CDS             complement(3619..4197)
                     /locus_tag="VKQ27_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HLY90057.1"
                     /translation="MLHRLYMRTLALAASPRATWWLFGIAFAEASFFPVPPDALLIPM
                     ALARPDRAWRFAVICTLGSVIGGALGYFIGYAVFDQLARPVLAFYGYGDRFAAFQDMY
                     ARWGLWVILIKGLTPIPYKIVTIASGAARFNFGLFMAASLVTRGARFFMVATLLRFFG
                     TPVRDFIERRLTLVTSLLALGVIGGFVVLRLF"
     gene            4293..4751
                     /locus_tag="VKQ27_13835"
     CDS             4293..4751
                     /locus_tag="VKQ27_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90058.1"
                     /translation="MRRIACLIVSIALLAPLPVRAQSSLPPHAWLFGAWIGGLYPPPS
                     TVTAQECLAQPVVIFTRDIVMRVVITDTNYTQRLVESARATGEGVEFRFARSVSPASG
                     PFGLNAGGPTDVGFGCTSPDILRVQRRSDNEISFPGCTDFPYPLIRCPGR"
     gene            4780..6456
                     /locus_tag="VKQ27_13840"
     CDS             4780..6456
                     /locus_tag="VKQ27_13840"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY90059.1"
                     /translation="MTRSVARLLAESLEAHDVDQVFCVPGESYVGLINAMSDMNSIRL
                     IVCRHEGGAGFMAVADGRLRKRAGCCIVSRGPGLSNSLVSLHSAYHDATPMVMLVGQV
                     ERKDFGRQALQEQNYSRLLSDVTKSVIEVIEPDQASEAIARAFHLAESGTPGPVAVVL
                     PEDLFDEKTDAEVDQPRPRVLAGPRADDLETLADMLAKSERPMILVGGALLADAIYDN
                     AAFGDLQRLAEEWVLPISPTHRRPQLFDATHPNYGGYMGIRVPPAQISEMKKADLMVS
                     IGERLTDTVSQSYSFPAAPQPQLPLVHVWPDANEIGRVFRPDLGIAASPHEVIKALLK
                     TGAPANAEKRRAWVNGLHAIHRKLLAKEWEPTTDGVNFAAIVCEVDKHLAPDATVTTD
                     AGNFGSFVHRYIGFKQGQIFLSSVVGAMGSGMPMAVAATLRRPGKQVVAFMGDGGALM
                     AGNEIATAMQYGGAPIVVISDNKMYGTIGMHHHVRYPGRPFQESMKLTNPDFAAWAKS
                     FGAEGITIRTDDEVADGIARAFSIKTKPVVVHCHTSAVQMSAWRRYSPPS"
     gene            6554..7528
                     /locus_tag="VKQ27_13845"
     CDS             6554..7528
                     /locus_tag="VKQ27_13845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90060.1"
                     /translation="MLPLDSTLRIGIQTIHRRTEPATAPWLPRIDELIDLVQLVDRSG
                     YDSLWVGDHISFAVPILDPLLQLAQAAVASRRLTLGTSVYLLPLRHPAPVAKQVSTLD
                     HLTEGRLIFGVGVGGEFPREYEVCGIPRNERGARLSEAIPLLRQFWSGEPVTHTGRFY
                     GPFTDVPMRPPTRQPGGPPIWCGGRTDGALRRTGRLADGWLSYVVTPEMYQAGLEKIA
                     AAAEGRSLQQFGTAHLLFARIDDTYEAALDAATETLSVRYAMDFRRAAQRYCALGRPD
                     QVAERIREFHAAGVRHIMLDLLGPYEQRDDQIARFAAEAMPLLRDLTQ"
     gene            7541..>8884
                     /locus_tag="VKQ27_13850"
     CDS             7541..>8884
                     /locus_tag="VKQ27_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLY90061.1"
                     /translation="MPRDLTAMLRPRSIALVGATDRSRWSQNTFDNLVNRKYAGAVHL
                     ISRRGGTVHGRAAATSCVALGEPVDLALLMVPQAAVDEAFADLAAANIHNAVILTSGF
                     AETGEDGVQLQAHLSALARQHDVSLLGPNCLGFVNFIDNVPLWTGGFRAPSQPGCIAV
                     VTQSGANGSFISSLAAQHEIGLSHMVSTGNEADLDCATFIDHLVNQPEVRAIALFAET
                     IRHAPSFAAAARRAIAAAKPIVVLKIGLSEVTARSAAAHTGALVGDDRVFDGVCRQLG
                     IVRVDSIEDLLFTADVISRTGVLKPDGLAAVSISGGACEIIADRSQVLNIPLPELSAT
                     ATAELRAALPSFGTPHNPLDITGAGVLQPDLFEQGLRILGKQPEYSVLACLFDVPVSA
                     ELATEFTRTALRHIGAGLRAASMPAFMVSHTGKPVTDVATQIIEEIGLNYISCGMH"
BASE COUNT         1632 a         3352 c         2450 g         1450 t
ORIGIN      
        1 acggcgagca cggtgacgcc gttcaccgtg aacacctggt acatctcggc cgcgcccgcg
       61 ccggtcaacg cgaccgcacc accaaacacc agcaatcgcc gcaccgccat gaaccgaggc
      121 gcactcgccg gtcgccggac cctcgccggt gcctcccgca gcgactgcgt cggcatcgcc
      181 agcggcgcct catccggcaa gccacggaag ctaacggcta cgtcgtccac cgataaatcc
      241 atgtctcggt caacggcgtc tcgccatcga tcagccgcgc atgcatctcg atcaggtttt
      301 cgctgccctg atcgagctgg aagctcagcc gcatcccttt aatgtccgga ttaggctcaa
      361 ccaccgggtt ttcgatcttc cccttgctcg cgcccacatc gatcgtcggc aacgcgtctg
      421 gcttccgctc ggccagcgtt ccgccagcaa aatcgatgac gaacagcctg ttcttctgat
      481 cccacgcaag gccgctgcgt gtcgccacaa ctttcgccac agactgcggc gtcggtgcag
      541 tccagcacca gtgcagccga taattcagca aatattcgcc cttcgccttc agcggatcat
      601 gcggccgcca gaacgcgacc atgttgtcgt tgacctcacg gtcgctgggt atttccacca
      661 gctcgacaat cccctggccc cagtcaccga tcggctcgat ccaggccgag ggccggttct
      721 cgtaatgcgc ctccagatcc tgaaagtcag agagctgtcg ctcgcgctgc atcagcccga
      781 aaccgcgcgt gccactgtcg gtgaaggcgc tgatctgcaa cgcacgcgga ttggccagcg
      841 gccgccacac atgctcgccc tttccagtcc ataacagcag cccattcgaa tcgtgcaccg
      901 catcgcggta gtcgtcggca cgcgcgcggt cattgctgtc gaacatgaac atcgacgtga
      961 gcggcgcgat gcccgccgtc gcaatgtcaa cccgtggata cagtgccatc tccgtgtcga
     1021 acaccgtctg cacgcccggc cggatggtga atcggaacgc cgccgccgcg ctctggctgt
     1081 cgagcagcgc atgtatcacg accgcgtcgg cgcccttcgc cggccgctcc agccagaacg
     1141 ccttgaacag aggaaattcc tcgcccgagg gctctgccgt cttgatcgcc agtccacgcg
     1201 ccgacaaccc gtacccctga tgcttcgcca cggcacgaaa gtagcttgcg cccaggaacg
     1261 cgctgacctc atcgaaatag tccggccggt tcatcggata atgcagcctg aacccggcaa
     1321 acccgatgtc cgccgtcggt ggcgtcacct tgtcgaaggt gaacatgctg gggttgtacc
     1381 ggatgtgcgt cgcgcgtccg ttcgtcacct cgtagatctg caccgcctcc ttgtacagat
     1441 agccgcggtg gaagaactcc gccgtgaagt gtgtcccctc gcctttccac agcgcgtgcg
     1501 cgggatcgaa ccgtatcgag cgatactgct gataatccag gttcttcagc gtatccggca
     1561 gcgtcgcgtc cggcgcctga taaggcttct gcgcgatctc gcgcgccatg ttccgtacag
     1621 tcgacccatc gaacgacgtg gtctcaccgg catacgcacg gccccctatc gccgcgacaa
     1681 tcggcagggc cgcgctccgc agcaacagct cgcgacgacg catggaaatt cccccaactc
     1741 aaaggatgcg cgcgttacat cccgtgattg gggcgaattc atgccgcatc aagcggagtt
     1801 gaacatgtca ttgcgaggaa cgcagcggtg gccgggcttg acccggctaa gcaatcccga
     1861 acggcctcgc gcaaacctca ggccgttgcc gcctccggct gccaggtccg ctgcagcgcg
     1921 cgcgccgagg ccagcaccac gtccgccgcc gccatctgga tcagcgaaca gaacaccgtc
     1981 ttcgtgctgt cgaagctgcc gttctcggcc aacttgttca tcggcgcgcc gccaccgcag
     2041 atggaaaaat actcgcagtt cgcgcggcac ttctccaccc cggcatcgac gtcccgctgc
     2101 atcgccgcca gccaccggct gtcacggatc tgctccatag agtcagtgtt gatattgccg
     2161 atgatgaagt cattgtattc cgcctgcttg taccccagca gctccggcga aaacgtcgat
     2221 acattcccca ggcaatccat attcagcatc gccagcggct cgacctgctg gttgcggaac
     2281 ggcgcggcat tcgggcggaa caccgcgttg atcatggtgt ccacctcgcg gacgaacttc
     2341 accttgtcgg aagcgcgcgc aagctgccag aaccgcgtca ggaaccgcct gaaccgcgtt
     2401 cgcacatcgc ccaccgccag catgtcggac gtatgctgcc cctccgactc ctccacgttg
     2461 aagcagacgt ggtcgatccc ctcggcgacg aagaacgcgt acatctcatc agccgcatcc
     2521 aggctgtcgc gcgacagcac cgagatcgtg tggaacggca tcctccgttc ccgcagcagc
     2581 cgcaccccgg cgatcgtctt gtcgaacgtc ccctgccccg accgcgtcac ccgatgccgg
     2641 tcatgcagat ggcgcggccc atcgatgctg atgccgatat cgacgtgcca ctcttcgata
     2701 agatcgcacc atgccggcgt cagcagcatc ccgttgctct ggaacgaatg catgatgtcg
     2761 atctcgcgcg gccgcagctt ctcgatcgcc ccgaacgcct cacgatagaa cgcgatcggt
     2821 gccaccagag gttcgcccgc atgccagatc acgttgaccg actggttcgc ccagcccgaa
     2881 gcgaacagct tcccgaacag attgcggacg gtgctctgcg cgatcaacgt ccgatcattg
     2941 cggctcggca gataacagta agtgcagtcg atgttgcaga agggcgtcgg ctgaacgacg
     3001 acgacgtcga tccgcggcgc ttcacgcccc acgcctaatt gcgccagccg ccgttatgcc
     3061 agttccccca tgggccgcca ttgcgccagc cgccgttgtg ccaaccgcca ttgccccagc
     3121 cgttgcgcca gccattgccc catggcgccg aattcaccca gccgccccag ccattgcccc
     3181 agggccgccc attgccccag gcggggtaac caccgttacc ccagacatgc ccgccattgc
     3241 cccatccaag gccgttgcca ttgagccacc aggccaggcg aatgtcggga tcagggatct
     3301 ggcccacatg gccacccagg atacccgcgc ttgcctcgtc caccgcatca cggatcgcct
     3361 gcagttcatt ggcaaccgag aatgtgtcga tatcaggctc ggtcctcggc tgctgcggct
     3421 cgaccggcgt cgccttggcc gcgctcgacg ccagcgccac cgacaaaccg aacgcaccgg
     3481 ccggtagcaa agccgtcagg aagcgcaaat accgatcgtg gcgtgacatg tttcctccga
     3541 gagagccggg ccgcgagttc ccgattcgaa ccgatgcact ctataatcga accccagtct
     3601 ttggcaccca ccaatttgtc agaacagccg cagcaccaca aatccgccaa tcacgcccag
     3661 cgccagtaaa ctcgtcacca acgtgaggcg ccgttcgatg aagtcgcgca ccggagtgcc
     3721 aaagaaccgc agcaacgttg cgaccatgaa aaagcgcgcg ccacgggtca ccaggctcgc
     3781 tgccatgaac aagccgaaat tgaagcgcgc cgcccccgag gcgatcgtga cgatcttata
     3841 cggaatcggc gtcagcccct tgatcaggat cacccacagg ccccagcgcg catacatgtc
     3901 ctggaatgcg gcgaaccggt cgccgtagcc atagaacgca agcacaggcc gtgccagctg
     3961 atcgaacacc gcgtatccga tgaaatatcc cagcgcgccg ccgatcacgc tccccaacgt
     4021 gcagatcacc gcaaaccgcc acgcccgatc gggccgcgcc agtgccatgg ggatcagcag
     4081 cgcatccggc ggcactggga aaaaactggc ctcagcaaaa gctatgccaa acagccacca
     4141 tgtcgcgcgc ggtgaagcag ccagcgcgag cgtgcgcata tacaagcggt gcagcatggg
     4201 cgcggttcct agcactgcca tccgcccgcc accactggtc actgattccg acccgcccta
     4261 tcgtgccgcc cgaacctacc gagggagcct gcatgcgccg catcgcctgt ctcatcgtct
     4321 ccatcgcgct gctggcgccg ctacccgtgc gggcgcagag cagcctgccg ccccacgcct
     4381 ggctgttcgg cgcctggatc ggtgggctgt atccaccgcc aagcaccgtc accgcccagg
     4441 aatgcctggc acagcccgtc gtcattttca cccgtgacat cgtgatgcgc gtggtgatca
     4501 ccgataccaa ctacacgcag cgcctggtcg aatccgcgcg tgccaccggc gagggcgtgg
     4561 aattccgctt cgcccgctcc gtgtcccctg cctccggccc attcggcctc aacgccggcg
     4621 gacccacgga tgtcggcttc ggatgcacca gccccgacat cctgcgggtg cagcgtcgca
     4681 gcgataacga gatctccttc ccaggctgca ccgatttccc ctacccgctg atacgctgcc
     4741 cgggccgctg aaaaagaacc ccaaggagaa acgtcccaaa tgactcgttc cgttgcccgc
     4801 ctgctcgccg agagcctgga ggcccacgac gtcgaccagg tcttctgcgt gcccggcgaa
     4861 agctatgtcg gcctgatcaa tgcgatgagc gacatgaact ccatccgcct catcgtctgc
     4921 cgccacgaag gcggtgccgg cttcatggcg gtcgcggacg gcaggctgcg caagcgcgcc
     4981 ggctgctgca tcgtctcgcg cggacccggc ctgtccaact cgctggtctc gctgcactcg
     5041 gcctaccacg acgccacgcc gatggtgatg ctggtaggcc aggtggaacg caaagacttc
     5101 ggccgccagg cgctgcagga acagaactac tcccgtctgc tgtccgacgt gacgaagtcg
     5161 gtgatcgagg tcatcgagcc cgaccaggcc tccgaagcca tcgcccgcgc cttccatctc
     5221 gcggaaagcg gcacgcccgg ccccgtcgcc gtcgtgctcc ccgaagacct gttcgacgaa
     5281 aagaccgatg cggaagtgga tcagccgcgt ccacgcgtcc tggcgggccc gcgcgccgat
     5341 gacctggaaa cactcgccga catgctggcc aaatcggaac gcccgatgat cctcgtcggc
     5401 ggcgccctgc tcgcggacgc catctacgac aacgccgcgt tcggcgacct gcagcgcctc
     5461 gccgaggaat gggtcctccc catctccccc acgcatcgcc gcccgcaact gttcgatgcg
     5521 acgcatccga actacggcgg ctacatgggc atccgcgttc cccccgcgca gatcagcgag
     5581 atgaagaagg ctgacctgat ggtctcgatc ggcgagcgcc tgaccgacac cgtcagccag
     5641 tcctacagct tccccgccgc accgcaacca cagcttcccc tcgtccacgt ctggcccgac
     5701 gcgaacgaaa tcggccgtgt attccgccct gatctcggca tcgccgcctc accgcatgaa
     5761 gtgatcaaag ctctgctgaa aacaggcgcc cccgccaacg ccgaaaagcg ccgcgcctgg
     5821 gtgaacggcc tgcacgcgat ccaccgcaag ctgctggcca aggaatggga acccaccacc
     5881 gacggcgtga acttcgcggc gatcgtctgc gaagtggaca agcacctcgc gccggatgcc
     5941 accgtcacca ccgacgccgg caatttcggc agcttcgtcc accgctatat cggcttcaag
     6001 caaggccaga tcttcctgtc atccgtcgtc ggcgccatgg gctccggcat gcccatggcc
     6061 gtcgccgcca cgctccgccg ccccggcaag caggtcgtcg cgttcatggg cgacggcggc
     6121 gcactcatgg ccggcaacga gatcgccacc gcaatgcagt acggcggagc ccccatcgtg
     6181 gtgatctcag acaacaagat gtacggcacc atcggcatgc accaccacgt gcgctacccg
     6241 ggccgcccct tccaggaatc gatgaagctt accaaccccg actttgccgc ctgggcgaaa
     6301 tccttcggcg cagagggcat caccatccgc acggacgacg aagtagccga cggcatcgct
     6361 cgcgccttct cgatcaagac gaaaccggtc gtagtccact gccacaccag cgccgtccag
     6421 atgagtgcct ggcgccgcta ctcgccaccg tcgtagcgcg cgccctcgcc ccgtgtcatt
     6481 gcgaggaacg gagtgacgaa gcaatctcga ctcgagattg cttcgcttcg ctcgcaatga
     6541 cagggtccct gcaatgctcc cactcgactc aacgctccgc atcggcatcc agaccatcca
     6601 tcgccgcacg gaacctgcca ccgccccctg gctgccgcgt atcgacgaac tcatagacct
     6661 cgtccagctc gttgatcgca gcggctacga ctcgctttgg gtcggcgacc acatctcctt
     6721 cgccgttccc atcctcgacc cgctgctcca gctcgcccag gccgccgtcg ccagccgccg
     6781 cctcactctc ggcacctcgg tgtatctact ccccctccgc caccccgccc ccgtcgccaa
     6841 gcaggtctcc accctcgacc acctcaccga aggccgcctc atcttcggcg tcggcgtcgg
     6901 cggcgaattc ccccgcgaat acgaagtctg cggtatcccc cgcaacgaac gcggcgcccg
     6961 cctcagcgaa gccatcccgc tgctccgtca attctggagc ggcgagcccg tcactcacac
     7021 cggtcgcttc tacggcccct tcaccgacgt gccgatgcgc ccgcccacgc gccagccggg
     7081 cggcccaccg atctggtgcg gcggccgaac cgatggcgcg ctccgccgca ccggtcgcct
     7141 cgccgatggc tggctctcct acgtcgtcac gcccgaaatg taccaagccg gcctggagaa
     7201 gatcgccgcg gccgccgaag gccgcagcct gcagcagttc ggcaccgcgc acctgctgtt
     7261 cgcccgcatc gacgacacct acgaagcggc actcgacgcc gccaccgaaa cactcagcgt
     7321 gcgctacgcc atggacttcc gccgcgccgc ccagcgctac tgcgcgctcg gccgccccga
     7381 ccaggtcgcc gaacgcatcc gcgaattcca tgccgccggt gttcggcata tcatgctcga
     7441 tctgctaggc ccgtacgaac aacgcgacga tcagatcgcg cgctttgcag ccgaggcaat
     7501 gccgctgctg cgcgacctga cccaataaga ggaaacgtcc atgccgcgcg acctgaccgc
     7561 catgctgcga ccgcgctcga tcgcgttggt cggcgcgacc gatcgctcgc gatggtcgca
     7621 gaacacgttc gacaacctgg tcaaccgtaa atacgccggc gccgtccacc tgatcagccg
     7681 ccgcggcggc accgtgcacg gccgcgccgc cgccacctcc tgcgtcgccc tcggcgaacc
     7741 ggtagacctc gccctgctga tggtcccgca ggccgccgtc gatgaagcct tcgccgatct
     7801 cgccgccgcc aacatccaca acgcggtgat cctcacctca ggcttcgccg aaacgggcga
     7861 ggacggggtg caactgcaag cccacctctc cgcactggca cgccagcacg atgtatcgct
     7921 gctcggcccc aactgcctcg gcttcgtcaa cttcatcgac aacgtgccac tgtggaccgg
     7981 cggcttccgc gccccgagcc agcccggctg catcgccgtc gtcacccaga gcggcgccaa
     8041 cggcagcttc atctccagcc tcgccgcgca gcacgagatc ggcctgtcgc acatggtctc
     8101 aaccggcaac gaagccgatc tggactgtgc aaccttcatc gatcatctcg tcaatcagcc
     8161 cgaagtccgc gcgatcgcgc tgttcgccga aaccatccgc cacgcgccaa gcttcgccgc
     8221 tgccgcccgc cgcgccatcg ccgccgccaa gccgatcgtc gtgctgaaga tcggcctgtc
     8281 cgaagtcacc gcacgttccg ccgccgcgca caccggcgcc ttggtcggcg atgaccgtgt
     8341 gttcgacggc gtctgccgcc agctcggcat cgtgcgcgtt gactcgatcg aggaccttct
     8401 cttcaccgcc gacgtcatct cccgcaccgg cgtgctgaaa cccgacggcc tcgccgccgt
     8461 ctccatctcc ggcggcgcct gcgagatcat cgccgaccgc tcccaggtcc tgaacatccc
     8521 gctccccgaa ctctccgcca ccgccactgc tgaactgcgg gccgccctgc ccagcttcgg
     8581 caccccgcac aatccgctgg acatcaccgg cgccggcgtc ctgcaacccg acctgttcga
     8641 gcaaggcctg cgcatcctcg gcaaacagcc cgaatattcg gtcctcgcct gcctgttcga
     8701 tgttcccgtc tccgccgagc tggcaaccga attcacccgc accgcgctgc gccacatcgg
     8761 cgccggcctg cgggccgcat cgatgcctgc gttcatggtc agccacaccg gcaagccggt
     8821 caccgacgtc gccacccaga tcatcgagga gatcggcctc aactacataa gctgcggcat
     8881 gcac
//
LOCUS       DATGEM010000433         5852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1148326, whole genome shotgun sequence.
ACCESSION   DATGEM010000433 DATGEM010000000
VERSION     DATGEM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148326"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VKQ27_13855"
     CDS             complement(<1..743)
                     /locus_tag="VKQ27_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90062.1"
                     /translation="MIRVASTAILLLGLAACSSPRPAPPQATTVPVANAAVVLAAGDA
                     SRDEGRFAEALQIYQQLLLRDQNDGAAQYGVAECMLGLGRAFDAKTAFDALPQDPRYH
                     AVALQGRGLAFLALNQRENAGKALREATELDQALWRSWNALGILADLQQDWPEAQTAY
                     AKALAAKPDSAMLHNNLGYSRLLSGQADAAIVEIRKAVGLDPDNATIQNNLRLAFATK
                     GNYRSATQGVAKEDQAMVLNNVGYVAMQRG"
     gene            complement(780..1406)
                     /locus_tag="VKQ27_13860"
     CDS             complement(780..1406)
                     /locus_tag="VKQ27_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90063.1"
                     /translation="MKTILGGALAVVFLVTACSDDATLEIRPSSFRTADAATPFDQAY
                     QNGKAHLMAGRLGLAIVSFEKALAIDPISVAALNACGAAYDALRRPDLGVRFYARALA
                     IEPDAPDTLNNMAISMLMAGKSDEAQRLFARAAELDPASRAIAGNRSRFALAASPQIA
                     DIPIDAEDRTRPHLERVGLAQYELGLPSARTRATHALPRAVAHPRFED"
     gene            complement(1403..2374)
                     /locus_tag="VKQ27_13865"
     CDS             complement(1403..2374)
                     /locus_tag="VKQ27_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLY90064.1"
                     /translation="MSERFCSSALDSSCSGAWPGSRCEDAMNILLDILVFAAVALGVF
                     GIASLVVPSGISAAPLAANSVLRGNSAAARHFARFSGSLLPSNLQERREVRLLFLQAD
                     FDAPKAAEIYYGIRVILALALPALTLGALALGFHDLRRMLPFALMAALIGFTVPALYV
                     SHRRKKNQQLVREGLPDVLDLLLVCSEAGLGLDMAIARVGEELALSQPLLSKHLRQIG
                     SELRAGRTRVDAMRGFADRTGTVEALSLVRLLVQSDIQGTSIVDALRTFSDEMRAHRT
                     LRAEEAAQKMSAKLSVILIFSFMPALFIAIGGPVVFKLFAALKGLAQ"
     gene            complement(2304..3266)
                     /locus_tag="VKQ27_13870"
     CDS             complement(2304..3266)
                     /locus_tag="VKQ27_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLY90065.1"
                     /translation="MFNGLLFVTTAAAIFALIQGVFIFADAQSLAQSSIRRRLTAIGV
                     GDLTGDALRRRMLGERASPWLASFLASRPMRWLDELIVTSGIGIPTERVLLGMTSALP
                     LALVILRLTGISPLFCIAGSVFAAFALPPWILYLVQQRRMKRLVHQLPEAIDILVRSL
                     RAGHPVATGVRLIAEELSDPIKTEFRLASDIMMYGLDIRSAFERMERRIPLEEFRYMA
                     AAIRIQYETGGNLAEILASLSNVMRERLRLQMKVKALSAEARLSGKILALVPFAVTGF
                     VSILNPDYFSETDKNPALAALLVGAVLLVGVGLVMLRRMARVKV"
     gene            complement(3276..4622)
                     /locus_tag="VKQ27_13875"
     CDS             complement(3276..4622)
                     /locus_tag="VKQ27_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HLY90066.1"
                     /translation="MSIFAKRSVPVPRSSLPMSDPVLADDRFITLKVNLHQRLIDVID
                     LSVIEKMPRELFESEIGDIIRDMLAKETTLLNDRERKQLVADILDELLGLGPLEPLLK
                     DASISDIIVNTHRQVFVERNGQIDMTSVRFKDEDHLVRIINKIVAAVGRRIDESQPIV
                     DARLADGSRVNAVIPPASVDGALLSIRKFAKLPYDLPRLVGAGTISPHIEKVLEVIVR
                     GRLNVLISGGTGAGKTTMLNAMSSAIGHRESLVTIEDAAELQLQQPMVRRLETRPENI
                     EGKGEITQRDLVKTALRMRPDRIIIGEVRGAEALDMLQAMNTGHVGSMSTIHANTPRD
                     ALSRLEQMISMAGFELAPHSIRSQIASAIDIVIQLARGPDGKRRVISLQEVEGMDGEV
                     VTTNEIFRFRRTATDENGIVHGQFESTGIRPRFANDVASLGLVLAEELFTPTTRSA"
     gene            complement(4634..5380)
                     /locus_tag="VKQ27_13880"
     CDS             complement(4634..5380)
                     /locus_tag="VKQ27_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90067.1"
                     /translation="MNGAAITFIKSGGGVGATALAVQMACACRGEIALLDLDIQFGSA
                     AFQMDITSTTSIIDLVATPGRLDEAMVRGAMVRPHECFDLLAAPQGIHPMEDLTPEGV
                     GQVIDVARATYASVLIDLPEIWSDWSYAALARSSQIVLVTRLTVPSLRQARRQMDMIK
                     QENLDRIPLFIIANQVEQGLFAKTVSKRKGEEVLGRKIDFAMPSDAAMQIASDSGLPL
                     GKVRGGGASVKKLARILHDILAGKPLPLAA"
     gene            complement(5393..5776)
                     /locus_tag="VKQ27_13885"
     CDS             complement(5393..5776)
                     /locus_tag="VKQ27_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HLY90068.1"
                     /translation="MTFAKDTAGAAALEFGLVLPVFLAVIIGIMQVAYMLWIDDLLHY
                     AVNGAARCGAVGSTVYPCAGSGATNMISTADALFGMAVASIPAGTFVANSACSGSGLT
                     ARYTVTFLLVAPVTLGASSCYPNYS"
BASE COUNT         1164 a         1782 c         1848 g         1058 t
ORIGIN      
        1 ccacgctgca tggcgacgta gccgacattg ttgagcacca tcgcctggtc ttccttggcc
       61 accccctggg ttgccgagcg gtaattgccc ttggtggcga aagccaggcg caaattgttc
      121 tggatcgtcg cattgtcggg atcgagcccg acggccttgc ggatctcgac gatcgccgca
      181 tcggcctgac cggacaggag ccgggaatag ccaagattgt tgtgaagcat ggcggagtcc
      241 ggcttggcgg caagggcctt ggcataggcc gtctgcgctt cgggccaatc ctgttgcaga
      301 tcggcgagta ttccgagggc gttccagctg cgccacagcg cctgatccaa ttccgtcgct
      361 tcacgcagag ccttccccgc attctcccgc tgattcaggg ccaggaaagc gaggccacgg
      421 ccctgcaacg ccaccgcatg atatcgggga tcttggggca gagcgtcgaa cgccgtcttg
      481 gcgtcgaacg ctctccccaa tccgagcatg cattccgcga cgccatattg ggcggcgccg
      541 tcgttctggt cccgcaacag aagttgctgg tagatctgca acgcctccgc aaatcgtccc
      601 tcatcgcggc tcgcatcgcc ggcggcaagg acaaccgccg cgttggcaac cgggacggtg
      661 gtggcttggg ggggggcagg ccttggactg gagcacgcgg ccaggcccag gaggagaatt
      721 gcagtgctcg ccactctgat catgtcaggc tccttcagat cgattaccac acatgacggt
      781 caatcctcga agcgagggtg tgcgaccgcc ctcggcagcg cgtgcgttgc gcgggttctc
      841 gcgctcggca atcccaattc atattgggcg agacccacgc gctcgagatg cggacgcgtc
      901 ctgtcttccg cgtcgatggg tatgtcagcg atctgcgggc tcgcggcgag cgcgaagcga
      961 gaccgattcc cggcgatcgc cctgctcgcc ggatcaagtt cggccgctcg cgcgaacaga
     1021 cgctgcgctt cgtccgattt tcccgccatg agcatggata tggccatgtt gttcagcgtg
     1081 tccggggcgt ccggttcgat cgccaacgcc cgcgcataga aacggacgcc gagatccggc
     1141 cgccgcaggg catcgtaggc tgcgccgcag gcgttgagcg ccgcgaccga aatcggatcg
     1201 atcgccaggg ctttttcgaa actgacgatc gccagaccca gccgtcccgc catgagatgc
     1261 gcctttccat tctggtaggc ctggtcaaag ggcgtcgcgg cgtcagccgt cctgaaggac
     1321 gatggacgaa tttccaaagt cgcgtcatcg ctgcaggcgg tcacgaggaa caccaccgcc
     1381 agagcgccac cgagaatcgt cttcattgcg ccaacccttt cagtgcggcg aagagcttga
     1441 acaccaccgg cccgccgatt gcgataaaga gcgccggcat gaaggagaaa atcaggatga
     1501 ccgaaagctt cgccgacatt ttttgggccg cctcttccgc tctcagcgtg cgatgcgccc
     1561 gcatttcatc ggaaaatgtt cgcagagcgt cgacgatgct cgtgccctgg atgtcggatt
     1621 gaacaaggag gcgcaccaag gatagcgcct ctactgtgcc ggtacggtcg gcgaatccgc
     1681 gcatcgcatc caccctcgtg cggccggcgc gcagctcgct gccgatctgc cggagatgtt
     1741 tcgacaatag cggctgcgac aaggccaact cctcgccaac gcgcgcgatc gccatgtcca
     1801 gccccaggcc ggcttccgag cagaccagca aaagatcgag cacgtcgggc aggccttcgc
     1861 ggaccaattg ctggttcttc ttgcgacgat ggctgacata caacgccggc accgtgaaac
     1921 cgatcagcgc cgccatgagg gcgaatggca gcatccggcg caagtcgtga aagcccaggg
     1981 cgagagcgcc cagggtcaat gccggcaagg cgagcgcgag aatgacgcga atgccgtaat
     2041 atatctcggc agccttgggc gcgtcgaaat ccgcctgcag aaacagcagc ctgacctccc
     2101 tccgctcctg taggttactg ggcagcagac tgccgctgaa gcgggcgaaa tggcgggcgg
     2161 ccgcagaatt cccgcgcagc acggaattgg cggcgagagg cgctgctgaa atccccgaag
     2221 gaaccacaag cgaggcaatg ccgaagacac cgagcgcgac cgcagcgaaa accaggatat
     2281 cgagaaggat attcatcgcg tcctcacacc ttgacccggg ccatgcgccg gagcatgacg
     2341 agtccaacgc cgacgagcag aaccgctccg acaagcaatg ctgcgagagc cgggttcttg
     2401 tcggtttcgg agaaatagtc aggatttaaa atgctgacga agccggtcac ggcaaagggg
     2461 accagggcca ggatcttgcc cgataatctc gcctcggccg acaatgcttt gaccttcatc
     2521 tgaagcctca ggcgctcacg catcacgttc gacagcgacg ccaggatttc ggcgagattg
     2581 ccgcctgtct cgtactggat acgaatggcg gcggccatat atctgaattc ctccaacgga
     2641 attcgccgtt ccatccgttc gaacgcgctg cggatgtcga gtccatacat catgatgtcg
     2701 gacgccaggc ggaactcggt tttgatcgga tcgctcagtt cctcggcgat caggcgcacg
     2761 cctgtcgcta ccggatggcc ggcgcgcagc gagcggacca ggatgtcgat cgcctcgggc
     2821 aactggtgaa ccaatctttt catgcgccgc tgctggacca gatacaggat ccaaggcggc
     2881 agcgcgaagg cggcaaagac gcttcccgcg atgcagaata gcgggctaat gccggtcagg
     2941 cgaagaatga cgagggcgag cggaagtgcc gatgtcatgc ccagcagcac ccgctcggtc
     3001 gggatcccga ttccactggt gacgatcagc tcatccagcc agcgcatcgg ccgggaagcg
     3061 agaaacgacg cgagccaggg cgaggcgcgc tcgccaagca ttcgacgacg cagggcgtca
     3121 ccggtcaaat cgccgacgcc gattgcggtc aaccggcgcc tgatgctgct ctgggcgagg
     3181 ctctgggcat cggcgaagat gaacacgccc tggataagag cgaagatcgc ggctgcggtg
     3241 gtgacgaaga gaagtccatt gaacatgatg cgcccctatg ccgatcgtgt ggtgggggta
     3301 aagagttcct cggccaggac cagccccagg cttgcgacgt cgttcgcaaa tctggggcga
     3361 atgccggtcg attcgaattg gccgtggaca atgccgtttt cgtcggtggc tgttcgccgg
     3421 aagcggaaga tctcgttggt ggtgaccacc tcgccgtcca tgccctcaac ctcctgcaga
     3481 gaaatgaccc gtcgcttgcc gtcgggaccg cgcgccaatt ggatgacgat gtcgatcgcc
     3541 gaagcgatct gggagcggat cgaatgaggc gccagttcga accccgccat gctgatcatc
     3601 tgctcaagcc gcgacagggc gtcgcgcggt gtattggcgt gaatggtgga catggagccg
     3661 acatggccgg tattcatggc ctgcagcatg tcgagggctt ccgcgccccg gacctcgccg
     3721 atgatgatgc ggtcgggacg catgcgcagg gccgtcttga ccaggtcgcg ttgcgtgatt
     3781 tcgcctttac cctcgatgtt ctccggccgc gtctcgagcc gccgcaccat cggctgctgc
     3841 agttgaagtt cggcggcatc ctcgatggtg acgaggcttt cgcggtgtcc gatcgcggag
     3901 gacatcgcgt tcagcatcgt cgtcttcccc gcacccgttc cgccggaaat aagaacgttg
     3961 agccgaccgc gaacgatgac ttcaagcact ttctcgatgt gaggcgaaat cgtgccggcc
     4021 cccacaagcc gtggcagatc gtatggtaat ttggcgaatt tccggatcga caggagggcg
     4081 ccgtcgacag aggcgggtgg aatgacggcg ttcacccgcg acccgtcggc gagtcgggca
     4141 tcgacgatcg gctggctttc gtcgattcgg cgaccgacgg ccgccacgat cttgttgatg
     4201 atgcgaacaa gatggtcttc gtccttgaag cggaccgacg tcatgtcgat ctgcccgttg
     4261 cgctcgacaa aaacctggcg atgcgtgttg acgatgatgt ctgaaatcga cgcatccttg
     4321 agcagcggct caaggggccc cagcccgaga agttcgtcga gaatatcggc caccagttgc
     4381 ttgcgctcgc gatcgttgag caaggtcgtc tctttcgcga gcatgtcgcg gatgatgtcg
     4441 ccgatttccg actcgaacag ctcgcgcggc attttctcga tgaccgaaag atcgatcacg
     4501 tcgataagcc tctggtggag attcactttg agggtgataa accggtcatc ggcaaggacg
     4561 gggtccgaca tgggaagcga cgagcgcggc accggaaccg accgcttggc gaaaatggac
     4621 atgacccgtc tccctatgcg gctagcggca ggggcttgcc tgccagaatg tcgtgaagga
     4681 tgcgcgcaag ttttttgacg gacgcgccgc cgccgcgcac cttgccgagg ggcagtccgg
     4741 aatccgaggc gatttgcatc gccgcgtcgc ttggcatggc aaagtcgatc ttgcggccga
     4801 gcacctcttc gcccttgcgc ttcgaaacgg tcttggcgaa cagcccctgc tcgacttgat
     4861 tggcgatgat gaagagcgga atgcgatcga ggttttcctg cttgatcatg tccatctggc
     4921 gacgcgcctg acgcagggac ggaacggtca ggcgggtgac gagcacgatc tgactcgatc
     4981 tggcgagcgc cgcatagctc caatcgctcc agatctccgg cagatcgatc agcacgctgg
     5041 cataggttgc gcgcgcaacg tcgatgacct gcccgactcc ctcgggggtg agatcttcca
     5101 tcgggtggat gccctgaggc gctgcaagca gatcgaagca ctcatggggc cgcaccatcg
     5161 ctcccctgac catcgcttcg tcgaggcgac ccggcgtggc gacgagatcg atgatgctgg
     5221 ttgtcgaggt gatatccatc tggaatgcgg cggagccgaa ctggatgtcg aggtcgagaa
     5281 gcgcaatctc gcctcggcac gcgcaagcca tctgaaccgc gagcgcggtg gcgccgacac
     5341 caccgccact cttgatgaaa gttatcgccg ctccgttcac catggacctc cgttacgagt
     5401 aattcgggta gcaggaactg gcaccgagtg tgaccggcgc aaccaataaa aacgtgaccg
     5461 tgtagcgggc ggtaagtcca cttccgctgc aggcggaatt ggcgacgaaa gttcccgccg
     5521 gaatcgatgc cacggccatg ccgaatagag catcggccgt ggaaatcatg ttggttgcgc
     5581 cgctgccggc acagggatag accgtcgagc ccaccgcccc gcaacgggcc gccccattga
     5641 ccgcgtagtg caacaggtcg tcgatccaaa gcatataggc cacctgcatg atcccaatga
     5701 tcacagccag gaagaccggg agaaccaggc cgaattcgag tgccgcggca ccggcggtgt
     5761 ctttggcgaa tgtcatggct taccggaccc gaaccgtggc agtggaggtc aagtggaggg
     5821 gaaagaccga cttcaggaac gtcgcgttca tc
//
LOCUS       DATGEM010000434         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_963533, whole genome shotgun sequence.
ACCESSION   DATGEM010000434 DATGEM010000000
VERSION     DATGEM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963533"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VKQ27_13890"
     CDS             <1..413
                     /locus_tag="VKQ27_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY90069.1"
                     /translation="IRFRSKLDPRLRELAILQVGWLARSPYEWSHHVKLGLDFGVSEA
                     DIQALIDDTAGKPTALDALTSTVLRAAREMTLDGAMADGTFAKLQQALGNELIVDLTV
                     TIGFYNAVVRLLATLQIDVEDDYMPYLKKFPLPA"
     gene            427..1545
                     /gene="ald"
                     /locus_tag="VKQ27_13895"
     CDS             427..1545
                     /gene="ald"
                     /locus_tag="VKQ27_13895"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLY90070.1"
                     /translation="MRIGVPKEIKTHEYRVGLTPAGVRELVMHGHSVSVEHNAAAGIG
                     IADDVYRAAGAAVVDTAAEVFATADMIVKVKEPQPQEIAMLRQGQVLFTYLHLAADKA
                     QTVGLMHTGATCIAYETVTDRAGALPLLAPMSEVAGRMSVQVGAHCLEKEQGGAGILL
                     GGVPGVAPAKVVVLGGGVSGTNAARMAVGLEASVTIIDKSLPRLKELDLQFGPRATTL
                     FATAESIEHQVLAADVVIGAVLVPGAAAPKLVTREMVRQMRPGSVLVDIAIDQGGCFA
                     TSRPTTHSTPTYVDDGVVHYCVTNMPGAVARSSTFALTNATLPFVIALAEKGWRKALV
                     DDPHLRHGLNVHAGKVTYQAVAEDLNLSYAPAETLLAA"
     gene            complement(1671..2870)
                     /locus_tag="VKQ27_13900"
     CDS             complement(1671..2870)
                     /locus_tag="VKQ27_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90071.1"
                     /translation="MNSQYRWVIVAAGAFMGCVAIGSIFSLPVFLQPMSQDTGWSRTA
                     ISSAMTIDFVTMGLASLGWGMVMDRLGPRVVVLGGSMLLGLGLVLASRASSVLEFQIF
                     YGILVGAGGGAIFAPMMATVTGWFDQHRSLAVSLVSAGMGIAPMTVAPIAARLVSTYD
                     WRFSQLAIGIAAWVLLLPAALLVRRSPALASTNGGRGAGGSRVSARAALMSPQFAILA
                     LTYFCCCATHSGPLFHTVSYAISCGLSVTAAVSIYSVEGLAGLFGRIAFGLLGDRLGA
                     KRVFVAGLFIQALAACSYMFAREQTEFYVVATVFGFVYAGIMPLYAVLMRENFPLPIM
                     GTMVGAGSMASSLGMALGPLAGGLIFDTYGNYARLYILSLGIGLGAAAIMATFRPARP
                     HALAVTG"
     gene            complement(2983..>3485)
                     /locus_tag="VKQ27_13905"
     CDS             complement(2983..>3485)
                     /locus_tag="VKQ27_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLY90072.1"
                     /translation="GEAHGDTAPSSEWAMHAAIYQACPSAQCVVHTHADACTALACLN
                     EALPAFHYMIANFGGDDVRCAPYVTFGTPELAQLAVEALAGRTACLLANHGMIVHATT
                     ADRALTNAIVLETLCRQYLLARSAGAVRLLTAEEMRAAHERYRTYGQRAAVTGTRAQE
                     GGKQAT"
BASE COUNT          700 a         1089 c         1124 g          572 t
ORIGIN      
        1 tcatccgctt ccgcagcaag ctcgatccgc ggctgcgcga actggcgatc ctgcaagtgg
       61 gctggctggc gcgctcgccc tatgagtggt cgcatcacgt caagctcggc ctcgatttcg
      121 gcgtgagcga ggccgacatc caggcactga tcgatgacac ggccggcaag ccgacggcgc
      181 tggatgcgct gaccagcact gtgctgcgcg cggcacgtga gatgaccctg gatggcgcga
      241 tggcggatgg cacgttcgct aaactgcaac aggcgctggg taatgagctg atcgtcgatc
      301 tgacggtcac gatcggattc tacaatgcgg tcgtacggct gctggccacc ctccagatcg
      361 acgttgagga tgactatatg ccttacctga agaagttccc gctgcccgcc tgacaggagg
      421 ttcctcgtgc gcattggcgt ccccaaggag atcaagacgc atgaataccg tgtcgggctg
      481 acgcccgctg gcgtgcgcga gcttgtcatg cacggccatt ccgtcagcgt cgagcacaac
      541 gctgcggctg gcattggcat tgccgatgat gtgtatcgtg cagccggcgc ggctgtcgtc
      601 gatactgctg ccgaggtatt cgcgaccgcc gatatgatcg tgaaggtgaa ggagccgcaa
      661 ccgcaggaga tcgcaatgct gcggcaagga caggtgctgt tcacttatct gcatctcgcg
      721 gccgacaagg cacagactgt aggtttgatg cacaccggcg ccacctgtat cgcctacgaa
      781 acggtgacgg atcgtgctgg tgccctgccg ttgctggcgc cgatgagcga ggtggccggg
      841 cggatgtcgg tccaggtggg cgcgcattgc ctggagaagg aacagggcgg cgccggtatc
      901 ctgctaggag gcgtgccggg tgttgcgccg gcgaaggtag tcgtgctggg cggcggcgtg
      961 tccggcacca atgcggcgcg catggcagtg ggtctggagg cgtctgtcac catcatcgac
     1021 aagtcactgc cgcggctgaa ggaactcgac ctgcaattcg gtccgcgcgc gaccaccctg
     1081 ttcgccaccg ctgagtcgat cgagcaccag gtgcttgcag ccgatgtggt gatcggtgcg
     1141 gtgctggtgc cgggcgcagc cgcgccgaag ctggtgaccc gcgagatggt gcggcagatg
     1201 cggccaggct ccgtgctggt ggatatcgcg atcgaccagg gcggttgctt cgccaccagc
     1261 cggcccacca cgcattccac accaacctat gtggacgatg gtgtggtgca ctactgcgtc
     1321 accaacatgc cgggagccgt ggcgcgcagt tccacgtttg cgctgaccaa tgcgacgctg
     1381 ccattcgtga tcgccctggc cgagaaggga tggcgcaagg ccctggtcga tgatccgcac
     1441 ctgcggcatg gcctgaatgt ccatgcgggg aaggttacgt atcaggcagt ggcagaggac
     1501 ctgaacctgt cctatgcgcc ggcggagacg ctcctggcgg cttagcttgg gctgagacca
     1561 tatctgagcg ggaccgatcg atgcttcccg tcatgccggg cgaaggcccg gcatccacgt
     1621 cttcgtgcct gtcacagtga gtcctcgtcc tcgggcactg gtatgagccg tcaaccggtc
     1681 acggcgagcg catgcggccg tgccggtctg aaggttgcca tgatcgcggc cgcgccaagg
     1741 ccaatgccaa gagagaggat gtagagccga gcgtagttgc cataggtgtc gaagatcaga
     1801 ccaccggcaa gcggccccaa agccatgcca agactcgaag ccatggaccc cgcgccaacc
     1861 attgtgccca tgatcggcag cgggaagttt tcccgcatga gcaccgcgta gagcggcatg
     1921 atgcccgcat acacgaaccc aaataccgtc gcgactacat agaattctgt ctgctcacgc
     1981 gcgaacatat aagagcaggc cgcgagtgcc tggatgaaca gtcccgcgac gaacacgcgc
     2041 tttgcaccaa ggcgatcgcc gagcagaccg aatgcgatcc gcccgaacaa gccagccaga
     2101 ccctcgacac tgtagatcga aaccgccgct gtaaccgaca ggccacagga gatcgcgtaa
     2161 ctgacggtat gaaacagcgg accggaatgg gtcgcgcaac agcagaagta tgtcagtgca
     2221 agaatagcga attgcggaga catcaatgcg gcgcgggcgc tcacccgcga gcctcccgca
     2281 ccacgtccgc cgttcgtgct cgccagcgct ggcgaccggc gcaccaggag agcagccggc
     2341 aacagcaata cccaggcagc gatgccaatc gcgagctggg aaaagcgcca gtcataggtc
     2401 gataccagac gggcagcgat cggcgcgacc gtcatgggtg caatccccat accggcggaa
     2461 accagcgaga ccgcaaggct gcgatgctga tcgaaccagc cggtaacggt ggccatcatc
     2521 ggcgcgaaga tcgcgccacc gccagcaccg acaaggatcc cgtagaagat ctggaactca
     2581 agaacactgg aggcgcggct ggcgagcaca agacccagcc caagcagcat actgcccccg
     2641 agcacgacga cccgcggacc caggcgatcc atcaccatgc cccagccgag cgaggcaagg
     2701 cccatcgtga cgaagtcgat cgtcatcgcg gacgatatgg ccgtgcgtga ccatccggta
     2761 tcctgcgaca tgggctgcaa gaagacggga agcgagaaga tcgagccaat cgcaacgcag
     2821 cccatgaagg cgccggcggc gacgatgacc cagcgatatt gggagttcat ggcccatccc
     2881 tttcgttgga agcatgagca gcggacagca ggcgcgatat caagttaacc gttcggttaa
     2941 atatggccat ccgcctgcca tgtcaagccg atcacctcag catcacgtgg cttgctttcc
     3001 cccctcctgg gcgcgagtcc cggtgacagc ggcgcgttgg ccgtaggtgc gataccgctc
     3061 gtgcgccgcg cgcatttcct cggcggtgag caggcgaacc gctcccgccg agcgcgccag
     3121 cagatactgc cggcatagcg tttccagcac gatcgcattg gtcagcgcgc gatcagccgt
     3181 tgtggcatgc acgatcatgc cgtgattggc gagcaggcac gctgtgcgtc ccgcaagcgc
     3241 ctcgaccgcc agttgcgcca gctccggtgt gccgaacgtg acgtagggcg cacaccgcac
     3301 gtcgtcaccg ccgaaattcg cgatcatgta gtggaacgcc ggcagcgctt cgttcagaca
     3361 ggccagcgcc gtgcaggcat cggcatgtgt atgcacaacg cactgcgccg acggacaagc
     3421 ctgataaatg gcggcgtgca tcgcccactc gctggagggc gcagtgtcgc cgtgcgcttc
     3481 gccac
//
LOCUS       DATGEM010000435        16000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1192984, whole genome shotgun sequence.
ACCESSION   DATGEM010000435 DATGEM010000000
VERSION     DATGEM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 16000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16000
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192984"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1121
                     /locus_tag="VKQ27_13910"
     CDS             171..1121
                     /locus_tag="VKQ27_13910"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY90073.1"
                     /translation="MPEGGSSIFTDADGYQAVIQDILDLLVLQPRAFHARLTWVDLPN
                     LQLLRAKEASARIGFLRLPSDQVFATFATAPNSTLHYNDIALQFGDLMLHSRGEFLHQ
                     RTTAACEWASIAITPAALSKFGRTLAGRTLMAPAAGQSVRPRRADGRRFERLHRRVCN
                     VVERKLDNLSNREVVRALEQDLIEALINCLAYGAGQQDRQASQNRPDVPRAFEALLAK
                     EPCRLLRTQEICTSLGISEATLRAKCSLALGMSPGQYQRLRRLKLLRTELLQARSSSE
                     SAIEDMVVRYGFSGLHRFVTEYWRIYGEMPPIHPVAPADR"
     gene            complement(1343..1747)
                     /locus_tag="VKQ27_13915"
     CDS             complement(1343..1747)
                     /locus_tag="VKQ27_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90074.1"
                     /translation="MNLLKLAAGAAMTIVVTCTVGLAQGAPEGRVYSFHSGPQGACPG
                     LDWHVVAQGNTLEGMIAWDNMKAMAHATGKLNPTAKSFEMTAKEVGGQGRTATITGSV
                     RADGWLVANINGPGVKCQSVTVQWFVPTQTNG"
     gene            complement(2223..2492)
                     /locus_tag="VKQ27_13920"
     CDS             complement(2223..2492)
                     /locus_tag="VKQ27_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90075.1"
                     /translation="MEKVPVELSEPAKGSVLMNKFWLGVLGAVVISTTLSLPASPAFA
                     LGGCGENYHRDGHGNCVFGGQDEDYCLKTKGHPATRMPNGTVVCK"
     gene            complement(2853..2929)
                     /locus_tag="VKQ27_13925"
     tRNA            complement(2853..2929)
                     /locus_tag="VKQ27_13925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2893..2895),aa:Arg,seq:acg)
     gene            3065..4381
                     /locus_tag="VKQ27_13930"
     CDS             3065..4381
                     /locus_tag="VKQ27_13930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90076.1"
                     /translation="MAPVNEKAIMQRVAWRLVPLLGIGYFVNALDRTNVAVAALTMNK
                     SLGFTAAEYGLGAGAFFWSYILFQVPSNVVLARLGARRWIATIMLAWGLCSGATALVT
                     GVSSFVVVRFLLGVAEAGYFSGVIFFMTRWFPDRYRCRAMGIFYAFAALAVSTGAPIS
                     GNILALHGWLGLQGWQWIFLIEAAPALLLAAICPFVLCDQPADAQWLTQDERRWLQSS
                     LLAEQRAAFGHSASIWQTLRSPCVVLLTIAYLCIGFGVYANVFFLPLIIKDLGYSNIT
                     VSYLAAIPAALGAAGMILVSRSSDRTGERLLHVTIPTLVAALGLIMTGLTIGRPLAEM
                     ISLSVVGCAISAALPTFWNLPTAWLGAGTAAAGIATINSIGNISGYLAPQLVGLLRDI
                     TGTYAAAMFVVGGMGLVAAALLPLAAATSARGLGAMDRREQPPAPA"
     gene            complement(4384..5496)
                     /gene="leuB"
                     /locus_tag="VKQ27_13935"
     CDS             complement(4384..5496)
                     /gene="leuB"
                     /locus_tag="VKQ27_13935"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLY90077.1"
                     /translation="MPANKKLLILPGDGIGPEVMREVRRVIDWMDRRRMVSFDVTDDL
                     VGGAAIDARGTPINDDTIQKAKEADAILFGSVGGPKWEKLGFQLRPELAILRLRKELD
                     LFANLRPATVLDPLVDASTLKADVVRGLDIMIVRESTGGIYFGEPRGIETLPSGEKRG
                     FDTETYTTSEIERVARVAFELARKRKNRVTSVEKANVMQSGLFWRQTVTTLHDREYQD
                     VELAHMYADNCAMQLVRNPRQFDVIVTSNIFGDLLSDLASMLTGSLGMLPSATLGPVA
                     ASGKRNALYEPIHGSAPDIAGKGIANPLAQIFSFAMLLRYSFGMDEDAALIERACTNV
                     LASGLRTADVMAPGTAKVGTNVMGESILRELDKLAA"
     gene            complement(5503..6123)
                     /gene="leuD"
                     /locus_tag="VKQ27_13940"
     CDS             complement(5503..6123)
                     /gene="leuD"
                     /locus_tag="VKQ27_13940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019084451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLY90078.1"
                     /translation="MEKFTTLTAVAAPMPMQNIDTDKVIPARFLKTIKRSGLGRHLFD
                     PLRFNADGSERADFILNRDGYRNAQIIVAHENFGCGSSREHAPWALQDFGIRCIIAPS
                     FGDIFYNNSFKNGLLPIRLPRQVCDELIEDAKLGGNARITIDLERQVVIRPNGEEIHF
                     DIAPLRKHLLMHGLDDIGQTLQHEQKISYHETRKREHHAWLPGTVG"
     gene            complement(6137..7555)
                     /gene="leuC"
                     /locus_tag="VKQ27_13945"
     CDS             complement(6137..7555)
                     /gene="leuC"
                     /locus_tag="VKQ27_13945"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLY90079.1"
                     /translation="MSEAKPRTLFDKIWDSHVVEKLPDGTCILYIDRHLLHEVTSPQA
                     FEGLRAAGRKLRRPDMTIAVADHNIPTVGPRTPADIAEEDSRIQVQTLEKNVVEFGVP
                     YIPILDVRQGIVHIIGPELGLSLPGTTIVCGDSHTSTHGAMGALAFGIGTSEVEHVMA
                     TQTLLQQPAKNMEIRVDGTLGFGCSGKDIVLAIIGKIGTAGGTGHVIEYTGEAIRALD
                     MAGRMTVSNMSIEAGARAGLVAPDETTFAYAKGREFAPKGEAWEQAVAYWRTLPSDPG
                     AAYDARVALNTADIAPMVTWGTTPGAVVPVTGVVPDPSGVEDEGLRSQMVRMLEYMAL
                     TPGQKLADVPIDVVFIGSCTNGRIEDIRAAAAVAKGRRVASGVQALVVPGSGQVKKQA
                     EEEGLDRILLEAGFEWRDPCCSMCLGMNPDKLTPGQRCASTSNRNFEGRQGPGGRTHL
                     VSPAMAAAAAVTGRLADVRQLG"
     gene            complement(7573..8001)
                     /gene="rplS"
                     /locus_tag="VKQ27_13950"
     CDS             complement(7573..8001)
                     /gene="rplS"
                     /locus_tag="VKQ27_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLY90080.1"
                     /translation="MNIIQRFEAEQIARLTTGRAVPQFAPGDTVRVSVKVVEGERTRT
                     QAFEGVCIGRSNKGLNSNFTVRKISYGEGVERVFPLYSPLIADIAVVRRGDVRRAKLY
                     YLRGRSGKSARIAEKARSTTTAGAAAAVEVPSTGEAERAE"
     gene            complement(7979..8716)
                     /gene="trmD"
                     /locus_tag="VKQ27_13955"
     CDS             complement(7979..8716)
                     /gene="trmD"
                     /locus_tag="VKQ27_13955"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLY90081.1"
                     /translation="MSWRASVLTLFPEMFPGPLALSLAGRALAAGVWSLQATNIRDFA
                     TDRHRTVDDAPFGGGAGMVMRPDVVDAAMASVADRRPVVFLTPRGRPLRQADAQRYAA
                     GLGVVLLCGRYEGIDQRVIEAHGMEEVSIGDYVLSGGELPALILLDAVVRLLPGVMGA
                     AESATEESFSEQLLEYPHYTRPADWHGRRVPEVLLSGHHAAIAAWRRAEAERITRERR
                     PDLWDAYTSATRYSDGRTKSDEHHPAL"
     gene            complement(8713..9246)
                     /gene="rimM"
                     /locus_tag="VKQ27_13960"
     CDS             complement(8713..9246)
                     /gene="rimM"
                     /locus_tag="VKQ27_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620838.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLY90082.1"
                     /translation="MPESRILLGVIGRPHGVRGLVRVTSYADDVTAYGALSDEHGRSF
                     VLRMRGEGVAEVAEVVGGVEVKVADRTAAEKLVNTRLYVERARLPEPEADEFYLADLI
                     GLMALDRGGATLGRVSAVHDYGAGASLEIEGAKPLIVPFTRACVPEVDVPGGRITVAE
                     PEAVDVASLPGPGSVAA"
     gene            complement(9252..9593)
                     /gene="rpsP"
                     /locus_tag="VKQ27_13965"
     CDS             complement(9252..9593)
                     /gene="rpsP"
                     /locus_tag="VKQ27_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLY90083.1"
                     /translation="MGLKIRLARAGAKKRPYYHIVVADSRSPRDGRFIEKLGSYNPML
                     PAEHEDRLRLQAERIQHWVSQGAVATDRVAKFLGQKGVGPMPSWGEQPVKSAPKKKAQ
                     ERAKAAEAAAG"
     gene            complement(9667..11055)
                     /gene="ffh"
                     /locus_tag="VKQ27_13970"
     CDS             complement(9667..11055)
                     /gene="ffh"
                     /locus_tag="VKQ27_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLY90084.1"
                     /translation="MFEALSDKLTGVFDRLRGRGALSEMDVGEALREVRVALLDADVA
                     LPVVRDFIAKVRERAVGAEVLSSVTPGQQVVKIVNDVLIKALGGEGAVPLNLNAPAPI
                     PILMVGLQGSGKTTTSGKLALRMRERLRRRVLLASLDTQRPAAQLQLAQLAEQAEVPS
                     LPIVAGQTPVQIAQRAMDVGRREGFDIVILDTAGRLSIDEALMDEVKQVRAATSPVET
                     LLVVDAMTGQDAVNTAKAFNDAVGVTGIVMTRMDGDARGGAALSMRAITGAPIKLIGV
                     GEKQDALEDFHPERVAGRILGQGDILGLVERAAEVVDQEEAEKLAAKMMRGKFDLEDY
                     ASQLKQITKMGSLSGILGMLPGIGKIKAQLEDANLDTVILKRQAAIISSMTQKERRTP
                     DIIKASRKKRIASGSGTSVQDVNKLLKQFDEMTTMMKRMNKLGQKGLMRGGLQALMPK
                     GMVPGGPGRRPF"
     gene            11390..11821
                     /locus_tag="VKQ27_13975"
     CDS             11390..11821
                     /locus_tag="VKQ27_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90085.1"
                     /translation="MLIALAAAPLSGCAYHDSSIAETAQRSLIGMPVGDLDLCAGLPT
                     KSEQINPATQVRSYERNESTNSGINLTFPVIGGGMNFGNGGYCHATFKLVNGEVAALS
                     YAGDTSSAGLPDAVCAPIVRTCVQQQTKHAEANTTAVASRH"
     gene            12201..12593
                     /locus_tag="VKQ27_13980"
     CDS             12201..12593
                     /locus_tag="VKQ27_13980"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HLY90086.1"
                     /translation="MPLARISLRRGKSAAHLAAIRDGIYAALRETFDVPENDRFMLVT
                     QHDADEFDYSPGYLNIARSDDLVMIQITVSNTRSIAKKQALYRRIVERLSDNPGLRPE
                     DVLINLVEVLPEDWSFGNGRASYVETAG"
     gene            complement(12678..13211)
                     /locus_tag="VKQ27_13985"
     CDS             complement(12678..13211)
                     /locus_tag="VKQ27_13985"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY90087.1"
                     /translation="MAALYADLVDYSRLLHQDDAGTVARLRGMHRLVRPAIRWHHGRL
                     VQTAGDSMLVTFESVREAVQCAVAIQHVLARENDGWPDDSSMRLRVGVDLGDVISDAR
                     GFHGSGIVIAVRLQEVCPPGGVCVSRAAHERGGDRLGLPFEPLGTLTLKNVPQPVDAF
                     VLRPRSGARPAELRLVE"
     gene            complement(13840..14466)
                     /locus_tag="VKQ27_13990"
     CDS             complement(13840..14466)
                     /locus_tag="VKQ27_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY90088.1"
                     /translation="MTLARGMERLETERLMLRRVSLADLPFYVRLHGMVEVARGLWLE
                     GRPKTAGQTEAWLRGTLTSYDALELGYLAVVRKADGALIGRCGLMDMAVEAAAPAGRL
                     RRGWFGREEVPEGVAVILECELGYTFDPAVWGQGYASEAARCVWDYARKGLGLGYVVS
                     AILPGNAASRRLAERARARMDGQMGAAGMVFERWVWGAETANRPNPLL"
     gene            complement(14569..15432)
                     /locus_tag="VKQ27_13995"
     CDS             complement(14569..15432)
                     /locus_tag="VKQ27_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLY90089.1"
                     /translation="MPRVDRRNRSRYWSSLGLSGLSMLEADFATHEYAPHRHDEFVIA
                     VTEVGGAIIKTGRETIEVRPDVLFVSNPAEPQSAWMGRSERWRYRSFYLSDAALAEVA
                     RHVGIDGAPRFPRSAVRDAELCQALLDLHRSPAAGQDSFEDVQMLISTLVRVFIRHGH
                     YDHRIQAELPERHSLDRVVECMREGFRDRLELVDLAGEAGMTVFQLIELFRRATGLTP
                     HAFLIQLRLSAARQELRHGASLAEVAIASGFYDQSALTKHFKRAYGITPGQYVNAVRS
                     DRITSAGSPRR"
     gene            15538..15858
                     /locus_tag="VKQ27_14000"
     CDS             15538..15858
                     /locus_tag="VKQ27_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90090.1"
                     /translation="MIARLWRGWAATSEAANLYQAFLRSTFLPSIHTIGGYRGAQILR
                     RTVGNEEEFTTITYFDSLDAIRRFAGEDCEAAHVAPRGRELLSRFDPRCLHYEVAVED
                     KPGF"
BASE COUNT         2931 a         5852 c         4371 g         2846 t
ORIGIN      
        1 agtccggcaa ttcccgatca gccaccgata ccggcgcgca ttccaatgcg atcattgccg
       61 attttgccat aggcgggacg cagccttgac tccaatctcc gtcgttcgac gtgcgggcgt
      121 gacgtgcctg atgctgcgaa ccgtacatcg cgactgcagg gcggaggcag atgccggaag
      181 gcggctccag catttttacg gatgccgacg gctaccaggc cgtcatccag gatattcttg
      241 acctcttggt gctgcagcca cgcgcctttc atgcccgtct gacctgggtc gatctaccca
      301 atctgcaact acttcgcgct aaggaagcat cggcgcgcat cgggttcctg cgtctccctt
      361 cagaccaggt tttcgcaacc ttcgcgacag caccgaattc gaccctgcat tataatgaca
      421 tcgcgctgca gttcggcgat ctcatgctgc atagccgcgg agagttcctg caccagcgga
      481 caacagccgc ctgcgagtgg gcatccatcg ccatcacacc ggctgcactt tcaaagttcg
      541 gcagaacgct tgccggacgg acgcttatgg caccggccgc tggccaatcc gtgcggcctc
      601 gccgcgctga tggccggcgg tttgaacgtt tgcacaggcg agtttgcaat gtggtggaga
      661 ggaaactcga taacctctcc aatcgagagg tcgtccgcgc gctggaacaa gacctgatcg
      721 aggcactgat aaattgcctg gcatatggag ccgggcaaca ggatcgccag gcttcacaga
      781 accgccctga cgtcccgcgg gcttttgaag cactgctcgc aaaagaaccc tgcaggcttc
      841 tgcgcacaca ggagatctgc acctcgctcg gcatatccga ggcaacgctg agagcgaaat
      901 gctcgctggc cctgggcatg agccccggtc aataccaacg gctcaggcgt ttgaaattgc
      961 tacggacaga gctgctgcag gcaagatcca gctcggaatc cgccatagaa gacatggtgg
     1021 ttcgctacgg cttctccggc ttgcaccgat ttgtcacgga atactggcgt atctacggcg
     1081 aaatgccgcc aatccatcca gttgctccag ccgacagata gcgcaggcta ccatatctta
     1141 ttgagcggga ccgaccgatg ctcttggccg ccgaaacggc tatcgaagga ggagagtaaa
     1201 gttcatgagc agcgaacccg cgcgcaagga aaccgggcgc ggcgatatgc tgcagcatgg
     1261 gaacggcgct ggcgcagacg gcggcacccg gccacggatt tggtgacggt cccaaggggg
     1321 cccgtcacca atgatctctg tgtcagccgt tggtctgtgt tgggacgaac cattgaacag
     1381 tgacgctctg acacttgacg ccaggcccgt tgatgttcgc gacgagccag ccatccgccc
     1441 ttacggatcc agtgatcgtg gccgttctgc cttgcccgcc gacttccttc gctgtcatct
     1501 cgaaagactt ggcggtcggg ttcaatttcc cggtcgcgtg tgccatggcc ttcatgttgt
     1561 cccaggcgat cataccctca agcgtgttcc cctgcgcgac gacgtgccag tccagaccag
     1621 gacacgcacc ttgcggaccc gagtggaagg aataaacacg cccctcgggc gcgccttggg
     1681 ccaacccgac ggtgcacgtg acaactatgg tcatcgcggc gccggcggcg agtttgagta
     1741 gattcatgtg cgcaatcctc ctattctttt gcttccggta ggttcggtga gactacggat
     1801 gcccgctcgc tgacgcctct caagcgcgcc aactccgtac agttacctac agtcgggcac
     1861 cgcgactatg cattttcggc aagcggctga ccggtattgt ccgtgcgctt cggacaatgc
     1921 aaaaaatccg ttgccgccac ggccgaagag atgaatcccg gatcgtcccc gatgacatag
     1981 cctgtttggt gtgggtcacg tgagcgaggc ggcccgccgc cacagctgca gcaccaagcc
     2041 caatacggag atatataccg ctcgcgactt gaatctgagc gtcccaatgg caagccattc
     2101 gaacgaacgc cgccatcgct tgttccaact cgttcggctc ttgccgccct tcgctgcctc
     2161 cctgacagcg aagggactat cgcttgcgct cgcagcctgg tccgccgtct cgacgacagc
     2221 gcttatttac agaccactgt cccatttggc atgcgagtgg ccggatggcc cttcgtcttg
     2281 aggcaatagt cttcgtcctg ccccccgaag acgcaattgc catgtccatc acgatggtag
     2341 ttctcgccgc agccgcccag tgcgaacgct ggcgaagccg gcaaagacag ggtggtggaa
     2401 ataacgacgg cacccagtac cccgagccag aacttgttca tcaacacgct ccccttggca
     2461 ggttcagaca actcgaccgg caccttctcc atgagacgac cgtcgctaaa cgttaggcac
     2521 gggttacaaa atggtcaaat gttccatgaa ccggattcgt gcggcacttt cgttagcttg
     2581 cggcaaagag atcggtgcga actggtaccg gtttgatcgt ctgtgcatcg ttccgctgag
     2641 tgcgcatcgt tcgctgcctc cgctccttac tgctccccag gaaacggcgg cagcgctcgc
     2701 gcatgctccg ttcgccggag caggcgcccc ccttcttgga gggggaaccg ataatccagc
     2761 agacagcggc cgcaccaaag aaccgcggcc cgttcgccca ctcaggtagc gcgctacatt
     2821 acaaaatatg cgtgagattt caatgaggta attggcgcgc ccgggaggat tcgaaccccc
     2881 aacccctaga tccgtagtct agtactctat ccaattgagc tacgggcgct tgccagggcg
     2941 cgcgggttta gccgaagcgc gacaacgggg caaggcttaa ccaagcgcac ggcggtttga
     3001 acgctttccc caccaacccg cgcctatgct ttcgccaaac cacgaaccgg gcgggaggaa
     3061 aaccatggca ccggtgaacg aaaaagccat catgcagcgg gttgcatggc gcctcgtgcc
     3121 cttgctcggc atcggctatt tcgtcaacgc gctggatcgg accaacgtgg ccgtcgcagc
     3181 gcttaccatg aacaagtcgc tcggcttcac tgcggccgaa tacggcctcg gtgccggcgc
     3241 cttcttctgg agctacatcc tcttccaggt gcccagcaac gtcgtgctgg ctcgcctcgg
     3301 agcccgccgc tggatcgcca ccatcatgct cgcctggggg ctctgctccg gcgcgaccgc
     3361 cctggtgacc ggcgtgagca gcttcgtcgt cgtccgcttc ctgttgggcg tcgccgaggc
     3421 aggctacttc tccggcgtga tcttcttcat gacccgctgg ttccccgacc gctatcgctg
     3481 ccgcgccatg ggcatcttct acgccttcgc cgcactcgcc gtgtccaccg gcgcgccgat
     3541 atcaggcaac atcctggcgc tgcacggctg gctcggcctc cagggctggc aatggatctt
     3601 cctgatcgag gctgcccctg cgcttctcct ggccgccatc tgccccttcg tgctgtgcga
     3661 tcagccggcc gatgcgcaat ggctgacaca agacgagaga cgatggctgc aatccagcct
     3721 cctcgccgag cagcgcgctg ccttcggcca cagtgccagc atatggcaga ccctgcgcag
     3781 tccctgcgtg gtcctgctga cgatcgccta tctctgcatc ggcttcggcg tctatgcaaa
     3841 cgtgttcttc ctgccgttga tcatcaagga tctcggctac agcaacatca ccgtcagcta
     3901 cctggccgct atccccgcgg cactcggcgc agccgggatg atcctcgttt cgcgcagttc
     3961 cgaccgcacc ggcgagcgcc ttctgcacgt cacgatcccc acgctggtcg ctgcactggg
     4021 attgatcatg acgggcctca ccatcggcag gccactcgcc gaaatgatct cgctctcagt
     4081 cgttggttgt gccatcagcg ccgcactgcc gacattctgg aacctgccga cagcctggct
     4141 cggagcaggc acagccgccg ccggcatcgc caccataaac tcaatcggca acatctccgg
     4201 ctatctcgcg ccgcaactgg ttggcttgct gcgcgacata acgggcacct acgcggccgc
     4261 catgttcgtt gtcggcggta tgggcctggt cgccgccgca ctgctgccgc ttgccgctgc
     4321 cacctccgct cgcggactcg gtgcgatgga ccgccgggag cagccgcctg cccccgcctg
     4381 acgttatgcc gcgagtttgt ccaactcgcg caggatcgac tcgcccatca cgttggtgcc
     4441 caccttcgcc gtccccggcg ccatgacgtc ggccgtccgc agcccagacg ccagcacatt
     4501 ggtgcacgcc cgctcgatca atgccgcatc ctcgtccatg ccgaacgaat aacgcagcag
     4561 catggcgaag ctgaaaatct gcgccagcgg attggcgatg cccttgccgg cgatatccgg
     4621 cgcggaccca tggatcggct cgtacagcgc attccgcttg cctgacgcgg ctaccggccc
     4681 aagcgtcgcc gagggcagca ttcccaggct cccggtcagc atcgacgcca gatccgacag
     4741 cagatcgccg aagatgttcg atgtgacgat cacatcgaat tgccgcggat tgcgcacaag
     4801 ctgcatcgcg cagttgtccg cgtacatgtg cgccagctca acatcctgat actcgcggtc
     4861 atgcagcgtc gtgaccgtct gccgccagaa cagacccgac tgcatcacat tggccttctc
     4921 cacgctggtg acgcggttct tgcgcttgcg ggccagttcg aacgccactc gcgccacgcg
     4981 ctcgatctcg ctcgtggtat aggtctcggt atcgaatcca cgcttttcgc cagacggcag
     5041 cgtctcgatc ccccgaggct caccgaagta aatcccccca gtgctctcgc gcacgatcat
     5101 gatgtcgagc ccgcgcacca cgtccgcctt cagtgtggag gcatccacca gcggatcaag
     5161 aaccgtcgcc ggccgcagat tggcgaacag gtcgagttcc ttgcgcaacc gcaggatcgc
     5221 caactccggc cgaagctgga acccgagctt ctcccacttc ggcccgccca ccgacccgaa
     5281 caggatcgcg tcggcctcct tcgccttctg aatcgtgtca tcattgatcg gcgtgccgcg
     5341 cgcatcgatc gcggcaccac ccaccaggtc atccgtgacg tcgaacgaca ccatgcgccg
     5401 tcgatccatc cagtcgatga cgcgacgcac ttctctcatg acctctgggc cgatcccgtc
     5461 accagggaga atcagcagct tcttgttcgc tggcatgctc tgttaaccca ccgtccctgg
     5521 cagccacgca tgatgctcgc gcttccgcgt ctcgtggtaa ctgatcttct gttcgtgctg
     5581 tagcgtctgg ccgatatcgt ccagcccatg catcagcaga tgcttccgca ggggcgcgat
     5641 atcgaagtgg atttcctcgc cattcggccg gatcaccacc tggcgctcca gatcgatagt
     5701 gatgcgcgca ttgcctccaa gcttcgcatc ctcgatcagc tcgtcgcaca cctgccgagg
     5761 cagccgtatc ggcagcagcc cgttcttgaa gctgttgtta tagaagatat cgccgaatga
     5821 aggcgcgatg atgcagcgga tgccaaagtc ctgcagcgcc cagggcgcgt gttcgcgcga
     5881 cgatccgcag ccgaaattct catgcgccac gatgatctgc gcattccgat agccatcccg
     5941 gttgaggatg aaatcggcac gctcgctgcc atccgcattg aaccgcaacg gatcgaacag
     6001 atgcctgccg agccccgacc gtttgatcgt cttcaagaat cgcgccggga tcaccttgtc
     6061 ggtatcgatg ttctgcatcg gcatcggcgc cgccacagca gtcagagtgg tgaatttttc
     6121 cacgttcgct cctccctcac cccagctgcc gaacgtcagc cagccgcccc gtcaccgccg
     6181 cggctgccgc catcgccggc gacaccagat gcgtccttcc gcccggcccc tgccgcccct
     6241 cgaaattgcg attggaagtg gacgcacacc gctgcccagg cgtcagcttg tcggggttca
     6301 tccccaagca catcgagcag catggatcgc gccactcaaa ccctgcctcc agcaggatcc
     6361 gatccagccc ctcctcctcg gcctgcttct tcacctgccc cgagcccggc accaccagcg
     6421 cctgcacgcc gctcgccacg cgccgcccct tcgcgaccgc cgccgccgcg cggatgtcct
     6481 cgatccgccc attcgtgcat gacccgatga acaccacgtc gatcggcaca tccgccagct
     6541 tctgcccggg cgtcagcgcc atgtactcca acatccgcac catctgcgac cgcagcccct
     6601 cgtcctccac acccgaagga tccggcacca cgcccgtcac cggcaccact gccccaggcg
     6661 tcgtccccca cgtcaccatc ggcgcgatat ctgcggtatt cagcgccaca cgcgcatcgt
     6721 acgcagcccc ggggtcgctt ggcagcgtgc gccagtaagc caccgcctgc tcccacgcct
     6781 cgcccttcgg cgcgaactcc cgccccttcg cataggcgaa cgtcgtctca tccggcgcga
     6841 ccaaccccgc ccgcgcgccc gcctcgatcg acatgttgga gacggtcatc cgccccgcca
     6901 tgtccagtgc gcggatcgcc tcgccggtat attcaatcac atggccagtg ccgcccgccg
     6961 tgccaatctt gccgatgatc gccagcacga tgtccttgcc cgagcagccg aaccccagcg
     7021 tgccatccac gcggatttcc atgttcttcg ccggttgctg caacagcgtc tgcgtcgcca
     7081 tcacgtgctc gacctcggac gtgccgatgc cgaacgccag tgcccccatc gccccatgcg
     7141 tggacgtatg cgaatccccg caaacgatcg tcgtccccgg cagcgacagc cccagctccg
     7201 gcccgatgat atgcacgatg ccctggcgca catcgaggat cggtatatac ggcaccccga
     7261 actcgacgac gttcttctcc agcgtctgca cctggatgcg gctgtcctct tcggcgatgt
     7321 cggccggcgt gcgcggcccc accgtcggaa tgttgtgatc cgccaccgcg atcgtcatgt
     7381 cgggccgccg cagcttgcgt cccgccgcgc gcagaccttc gaaggcctgc gggctggtca
     7441 cctcatgcag cagatgccgg tcgatgtaga gtatgcacgt cccgtccggg agcttctcca
     7501 cgacgtggct gtcccagatc ttgtcgaaca gcgtgcgtgg cttcgcttcg gacattgcgt
     7561 ttctctcctg tattattctg cgcgttcagc ttcgccggtg gacggcacct caacagcagc
     7621 ggcagcgcca gcagtcgtcg tgctccgcgc cttctccgca atacgcgccg acttgcccga
     7681 ccgaccgcgc aggtaataca gcttcgcccg ccgtacatcc ccgcgccgca ccaccgcgat
     7741 atcggcgatc agtggcgaat ataggggaaa cacgcgttcc acgccctcgc catagctgat
     7801 cttccgcacg gtgaagttgc tgttcagccc cttgttggag cggccgatgc atacgccctc
     7861 gaacgcctgc gtccgggtgc gctcaccttc caccaccttc accgagacgc gcacagtatc
     7921 gcccggcgcg aactgcggca ccgcacgccc cgtggtgagc cgggcaatct gctcggcctc
     7981 aaagcgctgg atgatgttca tcactcttcg tccttccgtc tgaatatctt gtcgctgatg
     8041 tgtaggcgtc ccacaagtcg ggccgccgct cgcgcgtgat ccgttccgct tccgcccgcc
     8101 gccaggcagc gatagcggca tggtgcccgg agagcagcac ctccggcacg cgccgaccgt
     8161 gccagtccgc cgggcgcgta tagtgcgggt attccagcaa ctgctcagaa aatgattctt
     8221 ccgtggcgct ttccgccgcg cccatcacgc caggcagcag ccgcaccacc gcatcgagca
     8281 gtatcagcgc cggcagttcc ccgcccgaca gcacgtaatc accgatgctg acctcttcca
     8341 tgccgtgcgc ctcgatcacc cgctgatcga taccctcata gcgcccgcac agcagcacca
     8401 ctccaagacc cgcggcataa cgctgcgcgt ctgcctgccg cagcggacgc ccgcgcggcg
     8461 tcagaaacac caccggccgt cgatccgcca ccgaggccat cgccgcatcc accacatccg
     8521 gacgcatcac catcccggct ccaccaccaa acggcgcatc atccaccgtc cggtgccgat
     8581 ccgtagcgaa atcccgtatg ttcgttgcct gcaatgacca tacgcccgcc gccagcgcgc
     8641 gccctgccag cgacagcgcc agaggccccg ggaacatctc cggaaacagc gtcagcacgg
     8701 aggcccgcca gctcatgccg ccaccgatcc aggtccaggg agcgacgcaa cgtccacggc
     8761 ctccggctcc gccaccgtga tccgcccgcc aggcacatcc acctccggca cgcacgcccg
     8821 ggtaaacgga acgatcagcg gtttcgcccc ctcaatttcc aggctggcgc ccgccccata
     8881 atcgtgcacc gcgctgaccc gccccagcgt cgccccgccg cggtcgagtg ccatgagccc
     8941 aatcagatcc gccaggtaga attcgtcagc ttccggttcc ggcagccgcg cccgctcgac
     9001 gtaaagccgc gtattcacca gcttctccgc cgcggtccga tccgccacct tcacctcaac
     9061 gccgccaacc acctcggcga cctcggcaac gccctcccct cgcatccgca gcacgaacga
     9121 ccgcccgtgc tcatcgctga gcgccccgta cgccgtcaca tcgtcggcgt acgacgtcac
     9181 ccgcaccagc ccccgcacac catgaggccg gccgatcacg cccaacagga ttcggctctc
     9241 aggcacagac cttacccagc cgctgcctcg gcagccttgg cccgttcctg cgccttcttc
     9301 ttcggcgccg acttgaccgg ctgctcgccc cagctcggca tcggcccgac gcccttctgc
     9361 cccaggaact tcgccacccg gtccgttgcc actgcccctt gcgacaccca gtgctgaatg
     9421 cgctcggctt gcagcctcag ccggtcctca tgctctgccg gcagcatcgg attgtagctg
     9481 cccagcttct cgatgaaccg cccatcccga gggctacggc tatccgccac cacgatgtgg
     9541 tagtacggcc gcttcttagc ccccgcgcga gccagtcgta tcttcaaacc catagatcca
     9601 tgtctccttc agacattcac aaacacagtc accgcccact cgccatggcc gggcctgacc
     9661 cggccatcag aacggccgcc gcccgggccc gcctggcacc atccccttcg gcatcagcgc
     9721 ctgcagcccg ccgcgcatca gccccttctg cccaagcttg ttcatccgct tcatcatcgt
     9781 cgtcatctcg tcgaactgct tcagcagctt gttcacatcc tgcacgctcg tgcccgatcc
     9841 cgaagcgatc cgcttcttcc ggctcgcctt gatgatgtcc ggcgtccgcc gctccttctg
     9901 cgtcatcgaa gaaatgatcg ccgcctgccg tttcaggatc accgtatcga ggttggcgtc
     9961 ctccagctga gccttgatct tcccgatccc cggcaacatc cccaggatcc ccgacaggct
    10021 ccccatcttg gtgatctgct tcagctgcga cgcgtaatcc tcgaggtcga acttcccgcg
    10081 catcatcttc gcggcaagct tctcggcctc ttcctggtcc accacctcgg cggcacgctc
    10141 caccaggcca aggatgtcgc cctgccccag gatccgcccc gccacacgct ccggatggaa
    10201 gtcctccagc gcgtcctgct tctcgcccac gccgatcagc ttgatcggcg cccccgtgat
    10261 cgcccgcatc gacagcgccg cgccaccacg cgcatcgcca tccatccgcg tcatcacgat
    10321 gccggtcacg cccaccgcgt cgttgaatgc cttggcggta ttgaccgcgt cctggccggt
    10381 catcgcgtca acgaccagca gcgtctccac cggcgaagtc gctgcgcgga cctgcttcac
    10441 ctcgtccatc agcgcctcgt cgatcgacaa ccgccccgcg gtgtcgagga tcacgatgtc
    10501 gaagccctcg cgccgcccga catccatcgc ccgctgcgcg atctgcaccg gcgtctgccc
    10561 cgccacgatc ggcaggctcg gcacctccgc ctgctcggcc agctgagcca actgcaactg
    10621 cgccgccggc cgctgcgtat cgaggctcgc cagcaacaca cgccgccgca gccgctcgcg
    10681 cattcgcagc gccagcttgc ccgacgtagt ggtcttcccc gacccctgca gacctaccat
    10741 caggatcggt atcggcgccg gcgcattcag gttgagcggc accgcgccct cgccgccaag
    10801 cgccttgatc agcacgtcgt tgacgatctt gaccacctgc tggcctggcg tgaccgagga
    10861 cagcacttcg gcccccaccg cccgctcgcg caccttggcg atgaagtcgc gcaccaccgg
    10921 cagcgccacg tcggcatcca gcagtgccac gcggacctcg cgcagtgcct cgccgacatc
    10981 catctcggac agcgccccac ggccgcgcag gcggtcgaac actccggtca gcttgtcaga
    11041 cagcgcttcg aacacgacac cactccaacg gccaaaacgc cgctgcgcga accctcgcgg
    11101 agcggcggag cccccgcatt caggcgggga ccgggacgac tacgttaccc ggatgcgccg
    11161 ggttatgcga gcggggggta ccgcaagtca atgggcgtca acgagcaatt ccccctctcc
    11221 cgcgcaacgg gggggcggcg cgtcggcacg ggaaagagcc cgccccgctt gccatccact
    11281 atccccagcc caatctccgc cgcagtgcag caatacgggc atgcgacagg cccagcctcg
    11341 ccacccccat cacgagtgga gaagccccat gcgagcccgc ctccccgcct tgctgatcgc
    11401 cctggccgcc gcaccgctgt ctggctgcgc ctaccacgac tccagcatcg ccgagacagc
    11461 ccagcgcagc ctgatcggca tgccggtggg tgatctcgac ctgtgtgccg gcctgccgac
    11521 caaatccgag cagatcaatc ctgcgaccca ggtccgcagc tacgagcgga acgagagcac
    11581 caacagcggt atcaacctca ccttccctgt gatcggcggc gggatgaact tcggcaacgg
    11641 cggctactgt cacgcaacct tcaagctggt gaacggcgag gtcgccgcgc tgagctacgc
    11701 cggcgacacg tcaagcgccg gcctgcccga cgcagtctgc gccccgatcg taaggacgtg
    11761 cgtgcagcag caaaccaaac acgcggaagc gaacacgacg gcggtcgcct cacggcacta
    11821 gcattccgac gctatcaaat gatgcacctc catgaaggac ggtgcttcct tgcgagagcc
    11881 gcgaataatt caacacaaag ctcacaaagg gcacgaagat cgcgaagtag gttttaggca
    11941 gcattcggcc gggtctaaac cgaaggtcca gaacagatct ccttggtgac cttcgcgttt
    12001 cttcgcaaac tttgtgttga atactgagaa tcccacgccg gatcgggcat tagagacgag
    12061 ggccaaatag ccagtccttg cccccacggg aataaccacg gtgctggtgc tccatcgctc
    12121 agggtcagag cactagctag caaccgaggc catcaaagtg catccccttg ggggcaagaa
    12181 tcatccgtcg gaggtcgccc atgccacttg cccgaatctc gctccgccgc ggcaagtccg
    12241 ccgcccacct ggccgcgatc cgcgacggta tctacgcggc cctgcgcgag acgttcgacg
    12301 tgccggagaa cgaccgcttc atgctggtaa cgcagcacga tgcggatgaa ttcgactatt
    12361 cgcccggcta cctgaacatc gcccgcagcg acgacctggt catgatccag atcaccgtca
    12421 gcaacacacg aagtatcgcg aagaagcagg cgctttaccg gcgcatcgta gaacgtctgt
    12481 ctgacaatcc cgggctgcgt cccgaggacg tgctcatcaa ccttgtcgaa gtcctgcccg
    12541 aggattggtc gtttggaaac ggccgcgctt cgtacgtgga gactgccggc tgagcagccg
    12601 atcgcgctcg cagcgcctca tgacccacac cacggatcag cgcctgcggg gcctccacgc
    12661 ccccgcaggc acagacccta ctcgaccagc cgcagttcag ccggccgtgc gccggaccgt
    12721 ggccgcagaa cgaacgcatc caccggctgc ggcacgttct tcagcgtcag cgtgcccagc
    12781 ggttcaaacg gcagtcccag ccgatcgcct ccccgctcgt gcgccgcacg cgagacgcag
    12841 acaccgccag gtgggcacac ctcctgcagt cgcaccgcga tcacgatgcc gctgccatgg
    12901 aagccccgcg catcgctgat cacatcgccc aggtcgacgc ccacgcgcag acgcatgctg
    12961 ctgtcatccg gccagccgtc attctcgcgc gccagcacgt gctggatggc caccgcgcac
    13021 tgcaccgctt cgcggacgct ctcgaacgtc acaagcatgg aatcgcccgc ggtctgcaca
    13081 agacgaccgt gatgccacct gatcgccggc cgtaccagcc gatgcatgcc acgcagccgc
    13141 gcaaccgtcc cggcatcatc ctgatgcagt agccgactgt agtcgacgag gtccgcgtag
    13201 agggctgcca tcaggcgcct tgcatggttg cctcgccgtg ccagcctgcc ggccagttca
    13261 tacggccagc tcagggttcg cgcaggacga tcaaactccc tggtcgcgct cctgacggat
    13321 agcgcgggca ccggcctcgg ctgataggga tccaggaatc gccgcaacat gacacatctc
    13381 ggaacctcga gtgcaatgcg gacaggacac tgagtgctat gaaaaagccc cgtccgatgg
    13441 ttgacggggc tgtgcgctac gagctgccga tgcagctacg gcgcgcgcga ccccgagccc
    13501 gcgataaagc gggcttggcg cgtaagacag cctgcgatga gtggcatggc gtacatgctc
    13561 tcctgttagc acaagccgta acaatcgtgg gtgtgaaact tatcacaccc tcgatcacag
    13621 aattgtgagc tgcagaacag ctgtgatatt ttatcaaatg aagccctgtt atcgtatcgg
    13681 cccatcgcgc cgatacccgg cattcgattg ccggtctgga tcggcttagg tgcgcaattc
    13741 ttgggttagc agaattgtat cgccttactg cggtgtcgac cgctgccggc caggaattag
    13801 accgctgtca acgaacacct gcattggggc agttccctct cagagcagag ggttcgggcg
    13861 gttcgccgtc tccgcccccc acacccaccg ctcgaacacc atgcccgccg ctcccatctg
    13921 cccatccatc cgagcccgcg cccgctccgc caaccggcgg gacgccgcgt tccccggcag
    13981 gatcgccgac accacgtatc ccagccccaa ccccttccgc gcataatccc aaacgcagcg
    14041 ggccgcttca ctcgcgtaac cctgccccca caccgccggg tcgaaagtgt acccgagttc
    14101 gcactccagg atcaccgcca cgccctccgg cacctcctcc cgcccgaacc acccccgccg
    14161 aagccgtccc gccggcgctg cggcctcaac cgccatgtcc atcagcccgc accgcccgat
    14221 cagcgcccca tccgccttcc gcaccaccgc caggtatccc agctccagcg cgtcgtagct
    14281 cgtcagagtc cctcgcagcc aagcctctgt ctgcccggcg gtcttcggcc gcccctccag
    14341 ccaaagcccc cgcgccactt ccaccatccc gtgcagccgc acatagaacg gcaggtccgc
    14401 caacgagacc cgccgcagca tcagccgctc cgtctccagc cgctccatcc cccgcgccag
    14461 cgtcatgccg ccgatgcctc tcgcctgagt acccggccgc ccaatgctgc cagatcgaac
    14521 acacaaccta tgcaacgact gaaccgtcgc atgcctgtct ggcaaacctc atcgccttgg
    14581 gctgccagcc gaagtgatgc ggtccgaccg aaccgcattc acatactgcc ctggcgtgat
    14641 gccataggcc cgcttgaagt gtttcgtcag cgcgctctga tcgtagaacc cagacgcaat
    14701 cgccacttcc gcaagcgatg cgccatgcct caattcctgt cgcgctgcac tcagccgtaa
    14761 ctggatcaga aacgcatgcg gcgtcaggcc ggtcgcacgt cgaaacagct cgatgagctg
    14821 aaagacagtc attcccgcct ccccagccag atcaaccagc tcgaggcgat cccgaaagcc
    14881 ctctctcatg cactccacga cccgatcgag cgaatgccgc tccggcagct cggcctgtat
    14941 tctatgatcg taatgaccat gccggatgaa aacgcgcacg agcgtggaaa tcagcatctg
    15001 cacgtcttcg aaactgtcct gcccagccgc cggagaccga tgcagatcga gcagcgcctg
    15061 acacaactcc gcatcgcgca cagcgctgcg tgggaagcgc ggtgcgccat cgatgcccac
    15121 gtgccgggct acctccgcaa gggcggcatc ggacagatag aacgagcggt accgccagcg
    15181 ctcgctccgc cccatccagg ccgactgtgg ctccgccgga ttcgagacaa acagcacgtc
    15241 agggcggacc tcgatcgtct ccctgccggt cttgatgatc gccccgccaa cctcggtgac
    15301 ggcaatgacg aattcatcgt gacggtgcgg ggcgtattcg tgtgtcgcga aatccgcctc
    15361 cagcatgctg aggccggaaa gccccagact gctccagtat ctgctcctgt tccgacgatc
    15421 gacccgaggc atcaacgacc tccgacaccg caaaatccgg tcgttcaatt tcgaccaatt
    15481 cagcacgcgc atcctcactt aaatacgcca caaggccaga cagcaacgga ggatccaatg
    15541 attgcgagac tatggcgggg atgggcggca acatctgaag cggcgaacct gtatcaggcg
    15601 tttctgcgct ccacgttcct gccatccatc cacacgatcg gcggctaccg cggcgcccag
    15661 atcctccgcc gcaccgtcgg caatgaggag gaattcacca ccatcactta cttcgactca
    15721 ctcgatgcga tccgccgctt cgccggcgaa gattgtgagg ccgcccacgt tgcgccgcga
    15781 gggcgagaac tgctctcgcg cttcgatcct cgctgcctgc attacgaagt ggccgtggaa
    15841 gacaagcccg gattctgaag gggattatca ggagaaatcg cgccctgcgg tttcgacatc
    15901 aggtccggct tccccgtctc cacatcgacc ggcaccgaga tgtgctcctg cgcgatcagc
    15961 caccgcccgt tcaccccacg gtagccatcg gtgacgcgga
//
LOCUS       DATGEM010000436         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_185636, whole genome shotgun sequence.
ACCESSION   DATGEM010000436 DATGEM010000000
VERSION     DATGEM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185636"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VKQ27_14005"
     CDS             complement(<1..481)
                     /locus_tag="VKQ27_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLY90091.1"
                     /translation="MKLLRYGPVGAEKPALLDRDGSIRCLSSVVRDIDGFALSDAALK
                     MLAGLDTTSLPKVDPATRLGACVARPMNYVCIGLNYADHAAEAGMAVPKEPIIFLKSL
                     GAMCGPNDDVKLPRGSKKLDWEVELGIVIGKTARYVPENEALSHVAGYCVCNDVSERE
                     "
     gene            complement(494..1243)
                     /locus_tag="VKQ27_14010"
     CDS             complement(494..1243)
                     /locus_tag="VKQ27_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY90092.1"
                     /translation="MSGRLDGKTAFVTAAGQGIGRAIALAFAAAGARVIATDVDEAKV
                     RQIASDAIHTAKLNVLHAADIGQAAADAGPTDILFNCAGFVHQGTLLDAAEDEWDFAF
                     ELNARSMFRTMKAFLPGMLERGRGVILNMASVSSSVKGVPNRFIYSATKAAVIGMTRS
                     VATDYVTKGIRCNCLCPGTVHTPSLDERIEANAAQAGSVEAARAAFIDRQPMGRLGTP
                     EEIAELAVYLASDAAQFITGQAVVIDGGLTL"
     gene            complement(1256..>2040)
                     /locus_tag="VKQ27_14015"
     CDS             complement(1256..>2040)
                     /locus_tag="VKQ27_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90093.1"
                     /translation="RRTMGLGAVKLADMQEYIRIVQALWRGETVEMELEGKRRPIRFM
                     NTELGLINVEDPIPLYIAASGPRARALTAKLGAAWIDNVADVERGAATLEQMRGAWQE
                     AGHSRDELQAVAWIGGAVLEEGEPVDGPRGMALAAPRASMLLHRAADTALAGYPTPLA
                     MRPEFAAAAEGYIAHARGLEPKDAYYLANHRGHLMFVRPDERPFISAEMIRRTSFIGT
                     ERDLTQRIGALADAGFTQVVFSIPPGQEHAVEDWGRIRRAFG"
BASE COUNT          376 a          711 c          595 g          358 t
ORIGIN      
        1 actcgcgctc gctcacgtcg ttgcagacgc aatagccggc gacgtggctg agcgcctcgt
       61 tctccggcac atagcgcgcg gtcttaccga tgacgatacc aagctcgact tcccagtcca
      121 gcttcttcga cccgcgcggc agcttcacgt cgtcattcgg accgcacatc gcaccgagcg
      181 atttcaggaa aatgatgggt tccttcggca ccgccatgcc ggcttcggcc gcatgatccg
      241 catagttgag cccaatgcac acgtaattca tcggccgcgc cacgcaggca cccagacgcg
      301 tggctggatc gaccttcggc agactcgtcg tatcgagccc ggccagcatt ttcagcgccg
      361 catcggacag cgcgaagcca tcgatatcgc gcaccactga cgaaaggcag cggatgctgc
      421 catcacgatc gagcagcgcg ggcttttcgg cgccgactgg gccatagcgg agcagcttca
      481 tgtgcgtttc cccctacaag gtaaggccgc cgtcgatcac gacagcctgg ccggtgatga
      541 actgtgcggc gtcagatgcc agataaactg cgagttcggc aatctcctcc ggcgtaccca
      601 gtcgtcccat tggctggcgg tcgatgaagg ctgccctcgc cgcttcgaca gaccccgcct
      661 gcgctgcatt cgcctcgatc cgttcgtcca gactcggcgt atgcacggtg ccagggcaga
      721 gacaattgca gcggatgccc ttcgtaacgt agtcggtggc caccgatcgg gtcatgccga
      781 tcaccgcagc ctttgtcgca ctgtagatga agcggttggg cacgcctttc acgctgctcg
      841 aaaccgacgc catgttcagg atcacgccgc gcccgcgttc cagcattccc ggcaggaacg
      901 ccttcatggt gcggaacatg gagcgcgcgt tcagttcgaa cgcaaagtcc cattcatcct
      961 cggcagcgtc cagcaacgtg ccttgatgca caaagcccgc acagttgaac aggatatcgg
     1021 tgggacccgc atccgcggcg gcctgcccga tatcggccgc gtgcagcaca ttcagcttcg
     1081 cagtgtggat cgcatccgac gcgatctgac gcaccttcgc ctcatcgaca tcggtggcaa
     1141 taacgcgcgc gccggccgcg gcgaacgcaa gtgcaatggc gcggccgatc ccctggccgg
     1201 cagcagtgac gaacgcagtc ttgccgtcga ggcgtcccga catgatgcaa tctcctcagc
     1261 cgaatgcgcg acggatgcgg ccccaatcct cgacagcatg ctcctgccca ggcgggatgg
     1321 aaaagaccac ctgggtgaaa ccggcatccg cgagcgcacc aatacgctgc gtcaggtcac
     1381 gctcggtgcc gatgaagctg gtgcgtcgga tcatctcggc gctgataaag ggacgctcgt
     1441 ccggacgcac gaacatcaga tgcccgcggt ggtttgccag ataatacgca tccttcggtt
     1501 cgagcccccg cgcatgcgca atatagcctt ctgccgcggc ggcgaactcg ggtcgcatgg
     1561 ccagcggcgt gggatatcca gccaacgccg tatcggccgc gcgatgcagc agcatggacg
     1621 cccgaggcgc ggccagcgcc atgccacgtg gcccatcgac cggctcgccc tcttccagca
     1681 ccgccccacc gatccacgcc accgcctgta gttcatcgcg cgaatggcct gcttcctgcc
     1741 acgcaccgcg catctgctcc agcgtcgcgg caccgcgttc cacatcagcg acattgtcga
     1801 tccacgcggc gccgagcttc gcggtcagcg cacgcgcacg cgggcccgac gcggcgatat
     1861 aaagcgggat cgggtcctcg acgttgatca gcccaagctc ggtgttcatg aagcggatcg
     1921 gccgacgctt gccctccagt tccatctcca ccgtctcgcc gcgccacagt gcctggacga
     1981 tgcgtatata ttcctgcata tccgccagct tcaccgcgcc aagtcccatc gtgcggcggc
//
LOCUS       DATGEM010000437         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1036961, whole genome shotgun sequence.
ACCESSION   DATGEM010000437 DATGEM010000000
VERSION     DATGEM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036961"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VKQ27_14020"
     CDS             <1..243
                     /locus_tag="VKQ27_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLY90094.1"
                     /translation="EFTQKMQVNGVDVAQLYLDTLELGAKGYTVYRTVPPYPPVDHEI
                     DVAIQTIVSKQKSVKEAMAQAQQNALMQLKRAGVKL"
     gene            247..1149
                     /locus_tag="VKQ27_14025"
     CDS             247..1149
                     /locus_tag="VKQ27_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLY90095.1"
                     /translation="MAARAAVSWAAQQRRAFRFGLLPALLVLATVTLGPAIYLLVTSL
                     TPLTPVNPDTAFDFSDPLGNYRLIVATDEFWRSVWVQVELSVVTVVTQVMVGLGLALL
                     LDRPSRFLEAIRTGFLVPMVLPPIVVAIIWKVIYTPDISPMHHLLAWLGMPLHSLITD
                     ASTAIWAIAAADTWEWFPFTMLMLLAALQMVPKEPQEAARIDGANAMQLFWHVRLPYI
                     RPTLVVAALFRLIDSIKAFPLIYVLTDGGPGGATEVTNYYAFQQTFNFAYWGYGSAVA
                     TLMVVGVFVLSVLVNRLGGEAIDD"
     gene            1142..1948
                     /locus_tag="VKQ27_14030"
     CDS             1142..1948
                     /locus_tag="VKQ27_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLY90096.1"
                     /translation="MTRRRGLAEGFGVLTLGVVLLPALWLLQMSFRHNDDIMSARLSF
                     VPTLENYTALWNGQFPGSFVHSLLASGFSTVFSLLLGVPAAYALARWRFAGARQIGLW
                     ILATRMAPPIAFTIPFFLAFTWAGLMDTVTGLALIYVTFNLALVIWSMRGFFAAVPRS
                     LEEAAWIDGCSVWNAFMRIALPLAAPGLAATGILCFILSWNDFFFALILTRTRAMTAP
                     VAIVNFMQYAGWEWGKIAAAGVLVMLPVLVFTFLVRAYLVRGLMAGGVKE"
     gene            1991..2365
                     /locus_tag="VKQ27_14035"
     CDS             1991..2365
                     /locus_tag="VKQ27_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLY90097.1"
                     /translation="MSSESLDCMKCPAFCCRSAGYVEVSRNDIRRLAKHLKLTVRAFE
                     EKHIVEKTRKGSKRIKSGFATCQFLGENRRCGVYEARPKDCRGYVCWNQEDTTVFDFA
                     RFYQLSIAEIRQQDANAGAKPK"
     gene            2375..>2975
                     /locus_tag="VKQ27_14040"
     CDS             2375..>2975
                     /locus_tag="VKQ27_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HLY90098.1"
                     /translation="MSDPEILALRAIIAKRARSDDIAQRRRDIDERGLQYKLAADARV
                     EAVSANGVKAEWSATADADPAKVVLYLHGGGYVIGSLDSHRHLVVEIGRAARGRTLAI
                     DYRMAPEHPFPAAVDDALAAYRFLLAEGVQPAAITIAGDSAGGGLVVAAMVAIRDAGL
                     PQPACGWPISPWVDMEAIGGSMVSKAATDPTVQKAGILDM"
BASE COUNT          477 a          842 c         1043 g          613 t
ORIGIN      
        1 gagttcacgc agaagatgca ggtcaacggg gtggatgtgg cgcaacttta tctggacacg
       61 ctggagctcg gggcgaaggg ctacacggtg tatcgcacgg tcccgcccta tccgccggtc
      121 gatcatgaga tcgacgtcgc gatccagacg attgtttcga agcagaaatc cgtcaaggag
      181 gcgatggcac aggcgcagca gaacgcgctg atgcagttga agcgtgccgg cgtgaagctg
      241 taggcattgg cagcacgtgc ggcagtcagt tgggcggcgc agcagcggcg cgcgttccgt
      301 tttggcctgc tgccggcgct gcttgtgctg gcgacagtga cactggggcc ggcgatctac
      361 ctgttggtca ccagcctgac gccgctgaca ccggtcaatc cggataccgc gttcgatttc
      421 tccgatcctc tggggaacta ccgactgatc gttgccactg acgaattctg gcgctcggtc
      481 tgggtccagg tcgagctttc agtggtgact gtcgtcacgc aggttatggt tgggctgggt
      541 ttggcgctgt tgctggatcg gccgtcgcgc ttcctggaag cgatccgcac cggcttcctg
      601 gtgccgatgg tgttgccgcc tatcgtcgtg gcgatcatct ggaaggtcat ctacacgccg
      661 gacatcagcc caatgcatca tctgctggcg tggctgggga tgccgttaca ttcgctgatc
      721 accgatgcga gcacggcgat ctgggcgatt gccgcggcag acacgtggga atggttcccg
      781 ttcaccatgc tgatgctgct ggcagcgctg cagatggtgc ccaaggagcc gcaggaggcg
      841 gcgcgtatcg atggggcgaa cgcgatgcag ctgttctggc atgtacggct gccgtatata
      901 cggccgactc tggtggtggc ggcgctgttc cggctgatcg acagcatcaa ggcgtttccg
      961 ctgatctatg tgctgacgga tggcgggccg ggtggcgcca ccgaggtcac caactactat
     1021 gcgttccagc agaccttcaa cttcgcctat tggggctatg gcagcgcggt ggcgacgctg
     1081 atggtcgttg gcgtgttcgt attgtcggtc ttggtgaatc gcctgggggg cgaggcgatc
     1141 gatgactagg cgccgcggcc tggcggaggg attcggcgta ctgaccctgg gggtggtgct
     1201 gctgcccgcg ctgtggctgc tccagatgtc gttccgccat aatgacgaca tcatgagcgc
     1261 gcggttgagc ttcgtcccca ccctggagaa ttacaccgcg ttgtggaacg ggcagtttcc
     1321 cggctcgttc gtgcacagtc tgctggcgag tggcttctcc acggtgtttt ccctgttgct
     1381 tggcgtgccg gcggcgtacg cgctggcgcg gtggcggttc gccggggcgc ggcagatcgg
     1441 gctatggatc ctggcaacac gcatggctcc gccgatcgct ttcaccatcc cgttcttcct
     1501 ggcgttcacc tgggcagggc tgatggatac ggtcaccggc ctcgcgctga tctatgtgac
     1561 gttcaacctc gcgctggtga tctggtcgat gcgcggcttc ttcgcggcgg tgccgcgttc
     1621 actggaggag gcggcgtgga tcgatggctg cagcgtgtgg aacgcgttca tgcggatcgc
     1681 cctgccgctg gcggcgcctg ggctggcagc gacgggtatc ctgtgtttca tcctgtcgtg
     1741 gaacgacttc tttttcgcgc tgatcctgac gcggacgcgg gccatgacgg cgccggtcgc
     1801 catcgtcaat ttcatgcaat acgctggctg ggagtgggga aagatcgcag ccgccggcgt
     1861 tctggtgatg ctgccggtcc tggtcttcac attcttggtc cgtgcctatc tggtgcgcgg
     1921 gctgatggca ggtggcgtaa aagaataggc tctgcttgcc cgtttcccaa agcatcgcca
     1981 aactgcggtc atgtccagcg agtcgcttga ttgcatgaag tgcccggcat tctgctgccg
     2041 gtctgcgggc tacgtcgaag tcagccggaa cgatatccgc cgcctggcga agcacctgaa
     2101 actcacggtg cgcgcgttcg aggagaagca catcgtcgag aagacccgga agggcagcaa
     2161 gcgcatcaag agtggtttcg caacctgcca gttcctgggc gagaatcgcc gctgcggtgt
     2221 ctatgaagcc cggccgaagg actgccgcgg ttatgtctgc tggaaccagg aagacaccac
     2281 ggtgttcgac ttcgcccggt tctatcagct gtccattgct gagatccgtc agcaggacgc
     2341 caatgcgggc gcaaagccga aataggaggg gtgcatgagc gatccggaaa tcctcgcgct
     2401 gcgtgcgatc atcgccaagc gtgcgcggtc cgacgacatt gcgcagcggc ggcgcgacat
     2461 cgatgaacgt gggctgcaat acaagctggc ggcggatgcc agggtggaag ctgtcagtgc
     2521 caacggtgtc aaggcggagt ggtcggcgac ggcggacgcc gatccggcga aagtcgtgct
     2581 gtatctgcat ggcggcggct atgtgatcgg ctcgctcgac agccatcgcc acctggtggt
     2641 ggaaataggg cgcgcggcgc gggggcgcac gttggcgatc gattatcgga tggcgccgga
     2701 gcatccgttc cccgcggcgg tcgatgatgc cctggcggcg tatcgcttcc ttctggccga
     2761 gggcgttcaa cctgcggcga ttaccattgc aggtgactcg gctggcggcg ggctggtggt
     2821 cgcggcaatg gtggcgatcc gcgatgcggg actgccgcag ccggcgtgcg gctggccgat
     2881 ctcgccatgg gtggacatgg aagcgatcgg cggaagcatg gtgagcaagg cggcgaccga
     2941 tccgacggtg cagaaggccg gcatcctcga tatgg
//
LOCUS       DATGEM010000438         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_187669, whole genome shotgun sequence.
ACCESSION   DATGEM010000438 DATGEM010000000
VERSION     DATGEM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187669"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VKQ27_14045"
     CDS             <1..611
                     /locus_tag="VKQ27_14045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY90099.1"
                     /translation="TVMQNANVSEPIMAGGRMAGVRLADGPEFESDVVVNCAGRWVND
                     PVGDTTLHLPMAPTVGLLAFTPPVAARVGRVVRSPDIHVRPDGAGRLVCIDPAEVLPP
                     FEPFPSASTPVAIELVRRASRLLPSIGDVKPEAVRMAIRPIPADSYSAVGPMPRLDGY
                     YLAVTHSGVTMAPFLGKAVADEIVHGRVADALADFRPARFFN"
     gene            624..1682
                     /locus_tag="VKQ27_14050"
     CDS             624..1682
                     /locus_tag="VKQ27_14050"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY90100.1"
                     /translation="MSNMQDRAAWTGATIDYRSDGLHTLSTDEVAEIDAALHHLHGLG
                     DLDFPAITTTTFPLPTVGAFLTGLRDELRFGRGFVLLRGIPRERYSTDDMARIYFGLG
                     AYIGTPIPQSWQGELLGHVIDVSDIEPGARGYHAGGAQRFHTDSCDIVSLMCLQGAKS
                     GGASRICSAITVHRRLESERPDLLKVLHDDYVFRRREVDAEYGSGTLVRRVSIFARTG
                     GEVSVNLSGDYPNRAVAAGDAVMTAEQRDALDEVQRIASESYLDMSIGEGDIQFLNNR
                     TLLHGRTGYEDHREMARRRHLLRLWLQVPGWPEMPANQGMHRSDDHTGWLRQRTPFME
                     VPSRYLAEITHRQDELAA"
     gene            1959..2906
                     /locus_tag="VKQ27_14055"
     CDS             1959..2906
                     /locus_tag="VKQ27_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY90101.1"
                     /translation="MIPLPSPQQLRYLTALAESRHFGRAAQTCAVTQSTLSAGILTLE
                     RQLDAAILDRGAGKHVVFTPLGLELIERAGNALTALEAVAEAAAAARTPMSGPLRLGV
                     IPTIGPFLLPRLMPVLRGSFPRLRLYLREDTTARLVERLAANRLDMLLLALPCDCGGA
                     DSQPVARDEFLVALPPEHRLVSQEQVPVAALAGERLLLLEDGHCLRDQALAVCGLLAG
                     DREGHDGFAATSLHTLVQMVAGGLGVTLLPRIAVNAGVTDGTGVILRPLAAAGAWRTL
                     GLAWRPNAPRASEYRALVPLLSETCRDALASPPAAAAAE"
     gene            2911..3246
                     /locus_tag="VKQ27_14060"
     CDS             2911..3246
                     /locus_tag="VKQ27_14060"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY90102.1"
                     /translation="MSNSPYLFRASAHPLRAISDRLAVREAVSQFGIALRYQERAPDP
                     PGAPEADWCETGHSIFVLAGRLRYRFDDHEVEAGPGDMLHIPAGRAHRHKPKVLGDEV
                     VRYVLTEFG"
     gene            3305..4201
                     /locus_tag="VKQ27_14065"
     CDS             3305..4201
                     /locus_tag="VKQ27_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90103.1"
                     /translation="MQFLESVRSLLARFGPPPSAPPLPTASMAPLLVPAAELPEDDDS
                     EWPTARIGVAEALWGEGFLFPGGREEALRLAKPLGLSAASSLMFIGAGPGGAPRCITT
                     ELGVWVTGYESNTRLVAVANERNARAGLGRRAQVELWDPFAPQFPSHYYHHAIAIEPL
                     HGAPPDTLLGAVAAALKPSGQFVLVEAVGDKPFDPSDATIEHWAGLERRPADVPTELQ
                     ITKTLRRLGFDVRIAEDVSQRHMHQAMTGWGQAVHAIEGARPTVQQLALIVHEAELWL
                     ARLRLMRKGRLRLVRWHAIGKA"
     gene            4253..4378
                     /gene="ykgO"
                     /locus_tag="VKQ27_14070"
     CDS             4253..4378
                     /gene="ykgO"
                     /locus_tag="VKQ27_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HLY90104.1"
                     /translation="MRVRNSLKAAKMRDKNCRVVRRRGRVYVINKKNPRMKARQG"
     gene            complement(4423..5679)
                     /locus_tag="VKQ27_14075"
     CDS             complement(4423..5679)
                     /locus_tag="VKQ27_14075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLY90105.1"
                     /translation="MAGKKKVAVIGAGIVGMATASYLQRDGHDVVVIDPQLPGQGASF
                     GNAGCFNPSSVVPVATPDTWKNIPHYLSDPLGPLRIRWSYLPALAPWLLRLVRAGTPQ
                     RIEAQARALKTILAPCLDALLPLARDAGAEHLVARNGILIAYRSQKSWDADATAWDIR
                     RRNGTHWEELDADELRQFDPNLSRDFTRGKLVPGNGHCLDPGGLVTALAAAAQGNGGT
                     FLRRRATGFSFNGADLRAIQTPEGEVPADAAVIATGAHSLPLAAQLGDRVPLETERGY
                     HAMLRTPEVMPRVPTTDSEGKFVATPMADGVRFAGTVELAGLNAAPDWRRARILLQQA
                     SKLYPGLARAYSEDRYTVWMGHRPSLPDSLPVLGPSTRSRDVFYAFGHGHVGMTGGPY
                     TGKVIADLVAGRPSPIDLSPFRPGRF"
     gene            5726..>5966
                     /locus_tag="VKQ27_14080"
     CDS             5726..>5966
                     /locus_tag="VKQ27_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLY90106.1"
                     /translation="MIPPKDQIKICFAHAAYQMKASFDALNTGIASFELRDGETLDRR
                     IGEADVLVVSGMWHNGLIQLGGRLRFIQSIGSGTDQ"
BASE COUNT          959 a         1871 c         2080 g         1056 t
ORIGIN      
        1 cgacggtgat gcagaatgcg aacgtcagtg agccgatcat ggcgggaggg cgcatggccg
       61 gagtgcggct cgcagacggt ccggagtttg agagcgacgt ggtggtgaac tgtgccggga
      121 ggtgggtgaa cgatccggtt ggcgatacca cgctccatct ccctatggct ccaacggtcg
      181 ggttgctggc gttcacgcct ccggttgcgg cgcgtgtggg gcgtgtcgtg cggtcgccgg
      241 acatccatgt gcggccggat ggtgccggac gccttgtgtg cattgatccg gccgaggtgc
      301 tgccaccgtt cgagcccttc cccagcgctt cgacgccggt ggcgatcgag cttgtgcggc
      361 gggccagccg gctgctgcca tccattggcg acgtgaagcc ggaagcggtg cgcatggcga
      421 tccggccaat tccggcggac tcctattccg ctgtcgggcc gatgccgcgc ctcgatggct
      481 actatcttgc ggtgacgcac agcggtgtca ccatggcacc attcctgggc aaggctgtgg
      541 cggacgagat cgtgcacggt cgtgtcgcag acgcgctggc ggatttccgt ccggcgcgct
      601 tcttcaactg acgaggacgg ccgatgagca atatgcagga tcgcgcagcg tggacaggag
      661 ccacgatcga ctatcgctcg gacgggctgc atacgttgag tactgatgaa gtggcggaga
      721 tcgatgcagc gctgcatcac ctgcatggac tcggcgatct cgattttccg gcgattacaa
      781 cgacgacgtt tccgctgccg acagtagggg cgttcctgac gggcctgcgc gatgaactgc
      841 ggttcgggcg cgggttcgtg ctgctgcgcg gtattccgcg cgagcgatac tccaccgatg
      901 atatggcgcg aatctacttc gggcttggcg cgtatatcgg gacgcccata ccacagtcct
      961 ggcaagggga actgcttggg cacgtgatcg atgtgagcga tatcgagcct ggcgcccggg
     1021 gctaccacgc aggcggcgcg cagcggttcc acacggattc ctgcgatatc gtgtcattga
     1081 tgtgcttaca gggtgcgaaa tccggcggcg ccagccgtat atgcagcgcg atcacggtgc
     1141 atcgccggct ggagagcgag cggcctgacc tgctgaaggt gctgcacgac gactacgtgt
     1201 tccgccggcg cgaagtcgat gcggaatatg gcagcggcac gctagtaagg cgggtttcca
     1261 tctttgcgcg caccgggggc gaggtttccg tgaacctgtc gggcgattat ccgaaccgcg
     1321 cggtggccgc cggtgacgcg gtcatgacag cggaacagcg ggatgcgctg gatgaggtgc
     1381 agcggatcgc gtccgaatct tatctcgaca tgagcattgg tgagggcgac atccagttcc
     1441 tcaataaccg gacgctgctg cacgggcgca ccggttatga ggaccatcgc gagatggcgc
     1501 gccgccggca tctgctgcgg ctatggctgc aagtccccgg ctggccggag atgccagcca
     1561 accagggaat gcatcggtca gacgatcaca cgggctggct gcgtcagcgg acgcccttca
     1621 tggaagtgcc gtcacgctat ctggcggaga tcacgcaccg gcaggacgag ctggccgcgt
     1681 agagcgtgct cgtcgcgcag ctaactcgac gagattgctt cccccggcct tcgccggggg
     1741 tcgcaatgac gggaactcgg gcagactgac gccgctgctg gcggctctac gttcagtgtt
     1801 gcgacgacgg cttgcgactg ttcagcgatc aggccacgcc ggcacggtgc gcgttcatgg
     1861 ccttgttctg tccttgacag aaatggagtg cacacaagtg agcgattgca ggggcgggcg
     1921 ccgatgaatt agattcacgt gtttgattga gaaaatcgat gattccgctg cccagcccgc
     1981 aacagcttcg ctacctgacg gcgctcgccg aaagccggca tttcggccgg gcggcgcaga
     2041 cctgtgctgt gacgcaatcc acgctgtccg cggggatttt gacgctcgaa cggcagctcg
     2101 atgctgcgat cctcgatcgc ggcgcgggca agcacgtggt gttcacgccc ctggggctgg
     2161 agctgatcga gcgcgccggc aatgccctga cagcgctgga ggctgtggcg gaagcggcgg
     2221 ccgcggcacg gacccctatg agcggaccgc tgcggctggg agtgatcccc acgatcgggc
     2281 cgttcctgct gcctcggctg atgccggtgc tgcgtggcag ctttcctcgg ctgagactgt
     2341 atctgcgtga ggacaccacc gcccggttgg tggaacggct tgcggcaaac cggctggaca
     2401 tgctgcttct cgcgctgcct tgcgattgtg gtggcgccga cagccagcct gtcgcacgcg
     2461 acgagttcct ggtggcgctg ccgcctgaac atcggctcgt gtcgcaagag caagtcccgg
     2521 tggccgcact ggcgggcgag cggttgctgt tgctggagga tgggcactgc ctccgtgatc
     2581 aggcacttgc ggtatgcggg ctgctggccg gagatcgtga ggggcacgac ggctttgcag
     2641 cgaccagcct tcatacgctg gtgcagatgg tcgccggtgg gttgggcgtg acgctgctgc
     2701 ctcgtatcgc ggtgaatgct ggtgtcaccg acggcaccgg ggtcattctc cgcccgctcg
     2761 cggcggcagg cgcgtggcgc acgctgggtc tcgcctggcg ccccaacgcg cctcgcgcat
     2821 cggaatatcg cgcgctggtg ccgctgttat cggagacctg cagggacgcg ctggcttcac
     2881 ccccggccgc cgccgcggca gaatgatcgc atgagcaaca gcccctacct gttccgcgct
     2941 tcagcgcacc ccctgcgggc catctctgat cggctcgccg tgcgggaagc cgtctctcaa
     3001 ttcgggatcg cgctgcgcta ccaggagcgg gcgcccgatc cacccggcgc gcccgaggcg
     3061 gattggtgtg aaaccggcca ttcgattttc gtgctcgcag ggcgtctgcg ttaccggttc
     3121 gacgaccacg aagtggaggc aggcccgggc gacatgctgc atatccctgc cggccgggcg
     3181 catcgtcaca agccaaaggt ccttggcgac gaggtggtgc gctacgtgct gaccgaattc
     3241 ggctagaccc tcaacgcttc gtcaacgctt cggcgtcact ctccggcgtg ggggtgactg
     3301 cccgatgcag tttctggaga gcgtgcgaag tctgctggca cggtttggac cgccgccatc
     3361 agccccgccg cttcccaccg cgtcaatggc tccgctgctg gtgcctgccg cggaattgcc
     3421 cgaggatgac gactccgaat ggcccactgc gcgtatcggc gtagcggagg cactgtgggg
     3481 cgaaggcttc ctgttccccg gcggccggga agaagcgctg cgcctggcca agccacttgg
     3541 gctgtctgct gcttccagcc tgatgttcat tggcgccggt ccaggcggcg cgccacgctg
     3601 catcacaacg gaacttggcg tttgggtcac cggctacgaa agcaacaccc gtctcgttgc
     3661 ggtggccaat gagcgcaacg cacgcgcggg gctcggacgg cgcgctcagg tggaactctg
     3721 ggatccgttc gcaccgcagt ttccgtcgca ctactaccac catgccattg ccatcgaacc
     3781 gctgcacggg gctccgcccg atacgctgct gggggccgtt gcggccgccc tgaaaccctc
     3841 gggccagttc gtgctggtcg aagcggtcgg cgacaagcca ttcgatccct cggacgcgac
     3901 gatcgagcac tgggcgggtc tggagcgccg cccggccgac gtgccaacgg aactgcagat
     3961 caccaagacg ctccgcaggc tcggcttcga tgtccgcatc gccgaggacg tgtcgcagcg
     4021 gcacatgcat caggcgatga ccggctgggg gcaggcggta cacgcgattg agggcgcgcg
     4081 gcccacggtg cagcagctcg ctcttatcgt gcacgaggcc gagctctggc tggcgcgcct
     4141 gcgcctgatg cgcaaagggc ggctacggct ggtgcggtgg cacgccatcg gcaaagcttg
     4201 acaggtgcaa tcctcccccc tatccagcgc cgctcttcac gaaggcgccg ctatgagagt
     4261 tcgcaacagc ctgaaagccg ccaagatgcg cgacaagaat tgtcgcgtgg tccgccgtcg
     4321 cggccgcgtc tacgtgatca acaagaagaa cccgcggatg aaggcccgcc agggttaggc
     4381 ctgctgggcg cgcctgcgcc ccccagtcca gaaccgccgg gttcagaacc gccctgggcg
     4441 gaatggtgac agatcaattg gtgacgggcg gccggcgacg aggtcggcga tgaccttgcc
     4501 ggtgtaaggg ccgccagtca tgccgacatg gccgtgtccg aacgcataga acacgtcgcg
     4561 cgaccgcgtt gacggaccga ggaccggaag cgagtccggc aggcttggcc ggtggcccat
     4621 ccagacggtg tagcggtcct ccgaataggc gcgcgccagc cccgggtaca gcttcgacgc
     4681 ctgctgcagc aggatacgcg cgcgacgcca gtcaggcgcg gcattcaggc cggccagttc
     4741 cacggttcca gcgaagcgca cgccatcggc catcggtgtc gcgacgaact tgccctctga
     4801 gtcggtggtc gggacgcgcg gcatcacttc cggcgttcgc agcatggcgt ggtagccgcg
     4861 ttcggtctcc agcggcaccc ggtcgccgag ttgcgcggcc agcgggaggg aatgagcgcc
     4921 ggtggcgatg accgcggcat cggctgggac ctcgccctct ggcgtctgga tcgcgcgaag
     4981 atccgcgccg ttgaagctga agccggtggc gcggcgtcgg aggaaggttc cgccgttgcc
     5041 ctgcgcggcc gcggccagtg ccgtgaccag tccgccggga tcgaggcaat gcccgttgcc
     5101 cggcaccaac ttgccacggg tgaaatcgcg cgaaaggttg gggtcgaact ggcgcagttc
     5161 gtcggcgtcc agttcctccc aatgggtgcc gttacggcga cggatgtccc aggcggtggc
     5221 atccgcgtcc cacgactttt gtgagcggta ggcgatcagg atgccgttac gcgcgaccag
     5281 atgctcggcg cctgcgtcgc gcgccagcgg cagcagggcg tcgaggcatg gtgccaggat
     5341 ggtcttcagc gcgcgggcct gtgcctcgat gcgttgcggc gtgccggcgc ggacgaggcg
     5401 gagcagccag ggcgccagcg cgggcagata gctccagcgg atgcgcagcg ggcccagcgg
     5461 atcggacaga tagtggggga tgttcttcca ggtatccggt gtcgcgaccg gcacgacgga
     5521 ggatggattg aagcagcccg cgttgccgaa ggaagccccc tgccctggca gttgcggatc
     5581 gatcaccacc acatcgtggc cgtcccgctg cagatagctt gcggtggcca tgccgacgat
     5641 gccggcgccg atgaccgcga cctttttctt ccccgccatg gccggtattg tcgcggctca
     5701 ttcccgccgc agcaaggttg cccccatgat cccgcccaag gaccagatca agatctgttt
     5761 cgcccatgcc gcgtatcaga tgaaggcgag cttcgacgcg ctgaataccg gcatcgccag
     5821 ttttgagttg cgcgatgggg agacgctgga tcgacggatt ggcgaggccg atgtgctggt
     5881 agtctccggc atgtggcaca acgggctgat ccagctgggt ggacggctac ggttcatcca
     5941 gtcgattgga tccggcaccg atcagt
//
LOCUS       DATGEM010000439         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_293002, whole genome shotgun sequence.
ACCESSION   DATGEM010000439 DATGEM010000000
VERSION     DATGEM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293002"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VKQ27_14085"
     CDS             <1..1034
                     /locus_tag="VKQ27_14085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90107.1"
                     /translation="IGAGLLLATLVREPWQLYATLGLLVGSGSVCLGYTGQGLFLPHW
                     FVRRRGLAMGLAFSGVGVGSVVLLPWLQTLITRTGWRTACMDLGIVTLVLLLPLNLLI
                     RRRPEDIGLAPDGGVSGNLATHDAAANVVDHAWVSVDWTLTRAIRTRRFWWIAIGYFF
                     ALFVWYAVQVHQTKYLAEIGFSPATAAWALGGVSLAGIPGGIALGHLSDRVGREFVWS
                     IGCLGFAATAALLLELRSTPTLPILYLMIAAQGVLGYGITSVISAIPAEIFQGRHYGT
                     IFGTLMLASIGGGAAGPWLTGALHDATGSYTIAFWIALIGSLVSAAAIWIAAPRKVRL
                     VAGRVARAG"
     gene            complement(1045..1974)
                     /locus_tag="VKQ27_14090"
     CDS             complement(1045..1974)
                     /locus_tag="VKQ27_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLY90108.1"
                     /translation="MPNKVIISCAVTGAIHTPSMSDYLPITPEEIARSSIEAAEAGAA
                     IIHLHARDPQTGEPTPDPAVFMRFLPVIKQSVDAVVNITTGGGLNMTLDDRLAAPLLA
                     QPEMCSLNMGSMNFNIARAGDRVKTWKHDWEKPYLDRTDDHIFRNTFRDIAGIVERLG
                     KAHGTKFEFECYDVGHLYNLAYCLDQKIVEPPLFVQTIFGILGGIGADTRNLLFMKET
                     ADKLFGDAYVWSVLAAGRHQMNFTTMAGILGGNVRVGLEDSLYIGRGALAKSNAEQVA
                     KIRRILEELSLEIASPKEAREMLNLKGGDRVGF"
BASE COUNT          337 a          695 c          641 g          430 t
ORIGIN      
        1 tcatcggcgc tggactgctc ctcgccacac tggtgcgcga gccgtggcag ctttacgcca
       61 cgctcggcct gctcgtcggc agtggcagtg tctgcctcgg ttataccggc caggggttgt
      121 ttctgccgca ctggttcgtc cgccgccgag gccttgccat gggcttggca ttctccggcg
      181 tcggggtcgg ctcagtggtc ctgctgccct ggctccagac gctgatcacg cgcaccggct
      241 ggcggaccgc gtgcatggac cttggcatcg tcaccttggt cctgctgctg ccgctgaacc
      301 tgctgatccg ccggcgcccg gaagacatag ggctcgcacc cgacggaggc gtatccggca
      361 acctggcaac ccacgatgcc gcggccaacg tcgtggacca cgcctgggtt tccgtcgatt
      421 ggactctcac acgtgccatt cgcacgcgac gcttctggtg gatcgccatc ggctatttct
      481 tcgcgctgtt cgtttggtac gcagtgcagg tgcaccagac caaatatctc gccgagatcg
      541 gcttcagccc agcaaccgcc gcctgggcac tgggcggtgt cagcctggcc ggaattcccg
      601 gcggcatcgc actcggccat ctgtccgacc gcgtcggtcg tgagttcgtc tggagcattg
      661 gctgccttgg tttcgcggca accgccgcgc tgctgcttga attgcgcagc acaccgacac
      721 tgccaatact gtatctgatg atcgcggccc agggtgtgct gggctacggc atcacatcgg
      781 tcatcagcgc catcccggcc gagatcttcc agggccgcca ctacggcacg atcttcggca
      841 cgctgatgct ggcttcaatc ggcggaggcg cggccggtcc atggctcacc ggtgcgctgc
      901 atgacgcgac cggtagctac acgattgcct tctggatcgc gctcatcggc agcctggtct
      961 cggcagccgc catctggatc gctgcaccgc gcaaggtccg gcttgtcgcc ggacgcgtcg
     1021 cgcgcgccgg ctgatcccgg ccggtcagaa cccgacccga tcgccgccct tgaggttgag
     1081 catctcgcgc gcctccttgg gcgaggcgat ctccaggctg agttcctcca ggatgcggcg
     1141 gatcttcgcc acctgctcgg cgttcgactt cgccagtgcg ccgcgaccga tatacaggct
     1201 gtcctccagc ccgacgcgca cattgccacc caggattcct gccatcgtcg tgaagttcat
     1261 ctggtgccgc cccgccgcca gcaccgacca gacataggca tcgccgaaca atttgtcggc
     1321 ggtctccttc atgaacagca gattgcgcgt atcagcaccg attccgccga gaatgccgaa
     1381 gatcgtctgc acgaacagcg gcggttcgac gatcttttga tcaaggcaat aagccaggtt
     1441 gtagagatgc cccacatcgt agcactcgaa ctcgaacttc gtgccatgtg ccttgccgag
     1501 acgttcgacg atacccgcga tgtcacggaa cgtgttgcgg aagatgtggt cgtcagtgcg
     1561 atccagatac ggcttttccc agtcatgttt ccatgtcttc acccggtcgc cggcacgcgc
     1621 gatgttgaaa ttcatgctcc ccatgttgag gctgcacatc tccggctgcg ccagcagtgg
     1681 cgccgcgagc cgatcatcca gcgtcatgtt tagaccaccg ccggtggtga tgttcaccac
     1741 cgcatcgacg ctctgcttga tcaccggcag gaagcgcatg aacaccgcgg gatcgggcgt
     1801 cggctcgccg gtctgcggat cgcgcgcatg gaggtggata atggcggcgc ctgcctccgc
     1861 ggcctcgatc gaggaacgcg cgatctcttc cggcgtgatc ggaaggtagt ccgacatgct
     1921 aggcgtgtgg atcgcgcccg tgacagcgca actgatgatg accttgttcg gcatgaggtt
     1981 cctcccggat cgcgatagct cgaatctttg ccctctgtta ggacgttggg ccagaggcta
     2041 taagcggtgg cggctggctg tccccgtagc tcagcaggat agagcacagg attcctaatc
     2101 ctg
//
LOCUS       DATGEM010000440         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_432895, whole genome shotgun sequence.
ACCESSION   DATGEM010000440 DATGEM010000000
VERSION     DATGEM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432895"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VKQ27_14095"
     CDS             complement(<1..879)
                     /locus_tag="VKQ27_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY90109.1"
                     /translation="MEDAARLLQLVVNGAAAGCIYGLIALGFVLIYKATEMINFAQGD
                     IMMLGGFVAFSLIAHFGLNYWIGSLLAIAIIAAFGFALDAIILRRVIGQPQFAVVMLT
                     LGLGFIFRAYAGITWGYDSVGFNTPFTNKTVNLNGMVLGLDNISIIVGTVLLCGVLYV
                     FFSRTRLGVAMQASSQNQLAAYYMGIPVRTVFSLIWAISAGVAAVAGILLSPVSMIDV
                     NMGFLGIKAFAAAVLGGFGSIPGALVGGVVIGIVEQLAGYFLPAGFQEVTSNVVLLAT
                     LILRPQGLFAESMGKRV"
     gene            complement(972..2129)
                     /locus_tag="VKQ27_14100"
     CDS             complement(972..2129)
                     /locus_tag="VKQ27_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY90110.1"
                     /translation="MSAIIEDLNELPDDDFRQHVRQWIEANYPPEIRNPPKRLHFKDN
                     KAWYHLLSRKGWLCPGWPKEFGGMGLSPGKQLIMIEEKERHGCTRLNDMGVTMLGPLL
                     IRYGTREQQDYYLPKILTGEHIWCQGYSEPNAGSDLASLRTEAVLDGEEWVVNGQKIW
                     TSLAMDANWIFLLVRTDKQAKKQEGISFLLVPMDSPGITVRPITNLDMEDEFCETFFD
                     NVRVPKGNLVGQINKGWTMAKNLLGFERIGSGSPRQSTYALGRLRQLADRMGVWDEDW
                     FQDRYTKLRLDLEDHKSLYSEFVARVYRGEALGPDVSMLKINQTELYQRITETMLEIS
                     AEYGGQFGPMDGNRELNPAGLFIQSRPSSIYAGSNEIQRNILAKNVLELPG"
     gene            complement(2142..4256)
                     /locus_tag="VKQ27_14105"
     CDS             complement(2142..4256)
                     /locus_tag="VKQ27_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLY90111.1"
                     /translation="MSAASPVSLVRQGDIGIITVNYPPVNALGPGVAEGIKDCLARGN
                     TDPAIKIMILIGAGRSFIAGADIRGFGTNRARLPLGERVYDVLDASEKPVIAAIHGYA
                     LGGGLEIALACHYRMALPSAKVGLPEVLIGILPGGGGTQRLPRLIGAKAALDMIVTGR
                     HVPAPEARRLGIIDELLPENADLLESAIAYATRIMEVRALPRVRDRQVKLDDPGIFDA
                     MRKSIARRARNQKAPYYCIDAVEASTTLPFDQGCRREADLFKELEDSAEARALRYAFF
                     IEREVARLPDIPANTPTRDFSTAAVVGAGTMGGGIAMSFADFGYPVKIMDVTPEALDR
                     GMQRIRDNYATSVKRGSLSEAEMQRRLARIEPVAGYEDIAECDLVIEAVFEEMDVKKP
                     VFARLDEVMKPGALIFSNTSALNIDALAEMTKRPKDVAGTHFFSPANVMKLLEVVRGK
                     ASSPETLATAMHVGRRIGKLSAMAGNTDGFVANRSRAPFNTEMVVLLEEGCLPEQVDK
                     VMVDFGYPMGPFAVGDLAGLDIGYASRKRRAAENPNFRKQPIPDRLVEMGRYGQKTGA
                     GWYKYEKGDRTPHPDPDVAAIIKEVAASLNIPQREFGDEEILRRLLFSSVNEACKILE
                     EGKAYRPSDVDVMWLHGFGFPRYRGGLFFWADTIGVRDVYNQIAAWHQQYGDRWAPSR
                     LLRDLAEAGTPFREAKAAAFVP"
     gene            complement(4441..>4842)
                     /locus_tag="VKQ27_14110"
     CDS             complement(4441..>4842)
                     /locus_tag="VKQ27_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90112.1"
                     /translation="ARDLDGFDLAPYVKIAMGDDLQACQDSVRPNLALYIGGMGARSK
                     NFYNDFAVRLGYEEAAAKIQDLYLGGHKKDAEALVPDKLIDEIALVGSTDRIKDRLQV
                     WKQAAKNGKLTTMVLTGATVEALRVVAEAVL"
BASE COUNT          928 a         1660 c         1369 g          885 t
ORIGIN      
        1 cacccgcttc cccatgcttt cggcgaacag gccctgtggc cgcaggatca gcgtcgccag
       61 cagcacgacg ttcgacgtca cctcctggaa gccagcgggc aggaaatagc cggcaagctg
      121 ttccacgatg ccgatcacca cgccaccgac cagcgcgccg gggatgctgc caaagccacc
      181 cagcaccgcg gcggcgaatg ccttgatgcc gaggaagccc atgttcacgt cgatcatgga
      241 aaccggcgac aacaggatgc ccgccaccgc ggccacgccg gcactgatcg cccagatcag
      301 cgagaacacc gtgcgcaccg ggatgcccat gtaatacgcc gccagctggt tctgcgacga
      361 tgcctgcatc gcgacaccca ggcgcgtgcg gctgaagaag acataaagca cgccgcacag
      421 cagaaccgtg ccgacgatga tcgatatgtt gtcgaggccg agcaccatgc cgttcaggtt
      481 gaccgtcttg ttggtgaacg gcgtattgaa cccgacggaa tcgtaccccc aggtgatccc
      541 ggcataggcg cggaaaatga agccaaggcc aagcgtcagc atcaccacgg caaactgtgg
      601 ctgtccgatg acccgtcgca ggatgatcgc gtccagggca aagccgaacg ccgcaatgat
      661 ggcaatggcg agtagtgaac cgatccagta gttcaacccg aaatgggcga tcaggctgaa
      721 cgcgacaaag ccgcccagca tcatgatgtc gccctgggcg aaattgatca tctcggtcgc
      781 cttgtagatc aggacgaacc cgagggcgat caggccgtag atacagccgg cagccgcacc
      841 gttgaccacg agttgcagca gacgtgccgc gtcttccaca gtcggcgtcc ctcccttcgg
      901 cggggtcgac gccccggcac ttgctttcaa acgtgacgat atgacgccgg tttgcgccag
      961 cgtcaacaca tctatcctgg aagctccagc acgttctttg ccaatatgtt acgctggatc
     1021 tcgttggacc cggcatagat cgacgaaggc cgggactgga taaaaagccc ggcggggttc
     1081 aactcgcggt tgccatccat cggtccgaac tggccgccat attcggcgga gatctccagc
     1141 atcgtttcgg taatgcgctg ataaagctcg gtctggttga tcttcagcat cgaaacgtcc
     1201 ggcccaagcg cctcccctcg gtacacccgg gcaacgaact cgctgtagag cgacttgtgg
     1261 tcctccaggt cgagccgcag tttcgtatag cggtcctgga accagtcctc gtcccacacg
     1321 cccatcctgt ccgccaactg ccgcagccgc ccaagcgcat aggttgactg tcgcggcgag
     1381 cccgatccga tgcgctcgaa ccccagcagg ttcttcgcca tcgtccagcc tttgttgatc
     1441 tgcccgacca gattgccttt cggcacgcgg acattatcga agaacgtttc gcagaactcg
     1501 tcctccatat cgaggttggt gatcggccgc accgtgatgc ccgggctgtc catgggcacc
     1561 agaaggaagc tgatcccctc ctgcttcttc gcctgcttgt cggtgcgcac gaggagaaag
     1621 atccagttgg cgtccatcgc cagcgaggtc cagatcttct ggccgttcac cacccattcc
     1681 tcgccatcca gcaccgcctc ggtgcgcaga ctggccagat cggaacccgc gttgggctcg
     1741 gaatatccct ggcaccagat atgctcgcca gtgaggatct tcggcagata gtagtcctgc
     1801 tgttccctgg tgccataccg gatcagcagc ggcccaagca tcgtcacgcc catgtcattg
     1861 agacgcgtgc agccgtggcg ctccttctcc tcgatcatga tgagttgctt gcctggcgaa
     1921 aggcccatgc cgccgaactc cttcggccag ccaggacaca gccatccctt gcgcgacagc
     1981 aaatggtacc aggctttgtt gtccttgaag tgcagccgct tgggcgggtt gcggatctcc
     2041 ggcggatagt tggcctcgat ccactgccgc acatgctggc ggaagtcgtc gtccggcagt
     2101 tcgttcaggt cttcgatgat ggcggacatg ctgtttcccc cctacggcac gaacgctgca
     2161 gccttggctt cccggaacgg ggtgcccgct tctgcgagat cccgcaacag ccgcgaaggc
     2221 gcccagcggt cgccgtattg ctgatgccag gcagcgatct ggttgtagac atcgcgcacg
     2281 ccgatcgtat cggcccagaa gaacaggccg ccgcgataac gcgggaagcc gaagccgtgc
     2341 agccacatca catccacatc gctcgggcga tacgccttgc cctcttccag gatcttgcaa
     2401 gcctcgttca ctgaagagaa cagcaggcgc cgcaggattt cctcatcgcc gaattcgcgc
     2461 tgcggaatgt tcaggctcgc ggcaacttcc ttgatgatcg ctgcaacgtc cggatccggg
     2521 tgcggtgtcc gatcaccctt ctcatatttg taccaaccag cgccggtctt ctgcccgtag
     2581 cggcccatct cgaccagccg atcagggatc ggctgcttgc ggaagttggg attttccgca
     2641 gcccgccgct tccggcttgc atagccaata tcaagacccg ccagatcgcc caccgcgaac
     2701 ggacccatcg gatacccgaa atcgaccatg accttgtcga cctgctccgg caggcaaccc
     2761 tcctccagca gaaccaccat ttccgtgttg aatggcgcgc gcgaacggtt ggcaacaaac
     2821 ccgtcggtgt tccccgccat cgccgacaac ttcccaatcc tcctgccgac gtgcatcgcg
     2881 gtcgccagcg tctccggcga cgaagccttc ccacgcacca cctccagcag cttcatcaca
     2941 ttggctggac tgaagaagtg cgtgcccgca acgtccttcg gccgcttggt catctcggca
     3001 agcgcgtcga tgttcagcgc cgaggtattg gagaagatca gtgcgcccgg cttcatcacc
     3061 tcatccagcc tggcgaatac cggcttcttc acatccatct cttcgaacac cgcctcgatc
     3121 accagatcgc actccgcgat gtcctcgtac cccgcgaccg gctcgatgcg tgcaagccgg
     3181 cgctgcatct ccgcttccga cagcgagccg cgcttcaccg aggtggcgta gttgtcgcgg
     3241 atgcgctgca tgccgcgatc cagcgcttcc ggcgtcacgt ccatgatctt caccggatag
     3301 ccgaagtccg cgaagctcat ggcgatgccg ccgcccatcg tccccgcgcc caccaccgcc
     3361 gccgtggaga aatcccgcgt cggcgtattt gccggtatgt caggcagacg cgccacttcg
     3421 cgctcgatga agaacgcata gcgcagcgca cgtgcctccg cgctgtcttc cagttccttg
     3481 aacagatcgg cctcgcgccg gcaaccctgg tcgaacggga gcgtcgtcga ggcctcgacg
     3541 gcatctatac agtaatacgg tgccttctgg ttgcgggcgc ggcgcgcgat cgacttgcgc
     3601 atcgcgtcga agatgccggg atcgtccagc ttcacctggc gatcgcgtac ccggggaagc
     3661 gcgcgaacct ccatgatgcg tgtggcgtac gcgattgcgc tctccagcag atcggcattc
     3721 tcgggcaaca gttcatcgat gatacccagc ctgcgcgctt ccggcgcagg cacatgccgg
     3781 ccggtgacaa tcatatccag cgctgccttg gcgccgatca ggcgaggcag acgctgcgtg
     3841 ccgccaccgc cgggcagaat gccgataagc acttccggca gtccgacctt cgcgctcggc
     3901 aacgccatgc gatagtgaca ggcaagcgcg atctccagtc cgccaccaag cgcgtagcca
     3961 tggatcgcgg cgatcaccgg tttctcgctg gcgtcaagaa catcgtagac acgctcgccc
     4021 aatgggagcc gcgcacgatt ggtgccgaag ccacggatat cagcgccggc gatgaagctg
     4081 cggccggcgc caatcaggat catgatcttg atcgccggat cggtgttgcc gcgcgccagg
     4141 cagtccttga tgccctccgc cacgccgggg ccgagagcat tcaccggcgg gtagttcaca
     4201 gtgatgatgc cgatatcgcc ctgacgaacc aggctgaccg gtgatgctgc actcatggat
     4261 gtttccctca acgcgacgcc caccaatgct aggagagcgg gcgcgccgcg tccatgctgg
     4321 ccggtatcag caaaacccgg ccagtagctt caccattccc gccccatgtc attgcgagaa
     4381 gcgaatgcga cgaagcgatc tcgaggcaag tcggcgccgc gagcgcgcgc gcaatgggcg
     4441 ctacagcacc gcctcggcca ccacgcgcag cgcctccacc gtggcaccgg tcagcaccat
     4501 cgtcgtcagc ttgccgttct tcgccgcctg cttccacacc tgcagccggt ccttgatccg
     4561 atccgttgaa ccgaccagcg cgatctcatc gatcaacttg tccggcacca gcgcctcggc
     4621 atccttctta tgcccgccga ggtacaggtc ctggatcttc gccgccgcct cttcatagcc
     4681 aagccgcacc gcgaaatcgt tgtagaaatt cttcgaccgc gcgcccatcc cgccgatata
     4741 cagcgccagg ttcggccgca ccgagtcctg gcacgcctgc agatcatccc ccatcgcgat
     4801 cttcacatac ggcgccagat cgaacccatc gagatcgcgc gc
//
LOCUS       DATGEM010000441         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1654959, whole genome shotgun sequence.
ACCESSION   DATGEM010000441 DATGEM010000000
VERSION     DATGEM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654959"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="alr"
                     /locus_tag="VKQ27_14115"
     CDS             complement(<1..938)
                     /gene="alr"
                     /locus_tag="VKQ27_14115"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLY90113.1"
                     /translation="MAAADRAGAVLEVDLGAIVANWHLLCALHRSGAVAGVVKADGYG
                     LGALAVAPALYSAGCRHFFVALLDEALAIRALVPEAMVAVLGGLIPGSEADYLAHDII
                     PVLGSSAEIDAWDVQAHRLERKLPAILHIDTGMSRLGLDARELAVLQQDHASLAALDL
                     RYVLTHLVSSEATHDPLNEHQRARFAAACAGLPAAPRSFANSSGIFLGEGWGSDLARP
                     GAALYGINPTPDRPSPMRQTVRLRARVLAVRDMQVGASVGYNATWSASRPSKIATAGI
                     GYADGLHRTLSNRGGAFFDGTPVPLVGRVSMDLTTF"
     gene            complement(925..>2343)
                     /locus_tag="VKQ27_14120"
     CDS             complement(925..>2343)
                     /locus_tag="VKQ27_14120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HLY90114.1"
                     /translation="GALLANNRAYERVSEFLSPEHFADPIHGRIYQAIVRRIEAGQLA
                     DAVTLKAEFEHSGILEEVGGTAYLVQLLTAMVGIINAGEYGRAIHDAWLRRQLIDIGE
                     TVVNNAFGADAELDGAQQIEAAEHTLFQLATDGSVSGGFVSFERALTEAIVGAERAFN
                     RTGHVSGLTTGLRDLDAKMGGLHGSDLIVLAGRPGMGKTSLATAVAFGAAKSLLAEAR
                     ATDPNAVAKRCIAIFSLEMSAEQLATRLLSEEAQVSSDRIRRGDISQRDFDKFVQVSR
                     EIASLPIEIDDTPAITLSALRTRARRLKRTKGLALIVVDYLQLMRPAAGTRPESRVLE
                     IGQLTQGLKAIAKELAVPVMALSQLSRAVESREDKRPQLSDLRESGTIEQDADSVMFI
                     YRDEYYLEQRMPKIAAFENDEKFQHAMDEWQQQMARVHNQADLIIAKQRHGPTGTIQL
                     FFEAEFTRFADLDRAHSDHGGG"
BASE COUNT          410 a          737 c          776 g          420 t
ORIGIN      
        1 tcgaaggtgg tcaggtccat ggagacacgc cctaccagcg gtaccggggt gccgtcaaag
       61 aaggcgccgc ctcgattgga gagcgttcga tgcaggccgt cggcatagcc gatcccggcg
      121 gtggcgattt tgctcggacg cgaggcgctc caggtcgcgt tgtaaccgac gcttgcgccc
      181 acttgcatgt cacggacggc aaggacgcga gcgcgcagcc tgaccgtctg gcgcatcgga
      241 ctgggacgat ccggcgtcgg gttgatgccg tagagcgcag cgccaggacg ggcgagatcg
      301 gaaccccagc cttcgccgag gaagatgcct gacgaattgg cgaagctgcg cggcgcggcg
      361 gggagaccgg cacatgcggc ggcgaaacgg gcgcgttgat gctcattcag tgggtcgtgc
      421 gtcgcttcgg atgacacgag atgcgtcagc acatagcgta ggtccagtgc ggcgaggctt
      481 gcatgatcct gctgcaggac cgccagttcg cgcgcatcga gaccgaggcg cgacatgccg
      541 gtatcgatat gcaggatcgc cgggagcttc cgctccagcc gatgcgcctg cacatcccac
      601 gcatcgattt ccgccgatga gcccaggacc gggatgatgt cgtgcgcgag gtagtcggcc
      661 tcgctgccgg gaatgagacc gcccaacact gcgaccatcg cttccggtac gagcgcgcgg
      721 atcgccagcg cctcatccaa gagtgcgacg aagaaatgcc ggcagcctgc cgagtacagc
      781 gctggtgcga cggcgagcgc gccaaggccg tagccgtctg ccttcaccac gccggcgacc
      841 gctccggagc gatgcagcgc acacaataaa tgccagtttg cgacgatcgc gccgagatcg
      901 acttccagaa ccgctccggc gcggtcagcc gccgccatgg tcggaatgtg cccggtcgag
      961 gtcggcgaag cgcgtgaact cggcctcgaa gaacaactgg atcgtgccgg tggggccgtg
     1021 gcgctgcttg gcgatgatca ggtcagcctg attgtggacg cgcgccatct gctgctgcca
     1081 ttcgtccatc gcatgctgga acttttcgtc gttctcgaac gccgcgatct tcggcatgcg
     1141 ttgttccagg tagtactcgt cgcggtaaat gaacatcacc gagtcggcgt cctgctcgat
     1201 ggtgcctgat tcgcgcaggt ccgagagctg aggccgctta tcctcacgcg attccacggc
     1261 gcgcgagagc tgcgacagcg ccatcaccgg caccgccaat tccttggcga ttgctttcag
     1321 gccctgggtg agctggccga tctccagcac gcggctttcc ggccgagtgc ccgcggctgg
     1381 gcgcatgagc tgaaggtagt ccaccacgat cagtgccagg cccttcgtgc gcttcaggcg
     1441 tcttgcgcgc gtgcggagtg ccgacagcgt gatcgccggc gtgtcatcga tctcgatagg
     1501 caacgatgcg atttcgcgtg atacctgcac gaacttatcg aaatcgcgct ggctgatatc
     1561 gccgcggcgg atgcggtcgc tggatacctg tgcttcctcg ctgagcagac gcgttgccaa
     1621 ctgctcggcg ctcatttcca gcgagaaaat ggcgatgcag cgcttggcga ccgcgttcgg
     1681 atcggtggcg cgtgcttccg ccagaaggga cttcgcggca ccgaaggcga cagccgtggc
     1741 gagcgatgtc ttgcccatgc cgggccgccc ggccagcacg atcaggtcgg agccatgcag
     1801 gccgcccatc tttgcgtcga ggtcccgtag cccagtcgtc aagcccgaaa catgacccgt
     1861 gcggttgaac gcgcgttcag cgccgacgat tgcttccgtg agagcccgct caaagctgac
     1921 gaagccgcca ctcaccgaac cgtccgtggc gagctggaac agtgtatgtt ccgccgcttc
     1981 gatctgctga gcgccgtcga gttccgcatc ggcgccgaac gcgttgttca ccactgtctc
     2041 gccgatatcg atcaactggc ggcgaagcca ggcgtcgtga attgcacggc catattcgcc
     2101 ggcattgatg atgccgacca ttgcggtgag gagctgcacc agataggcgg tgccgccgac
     2161 ttcctcgagg atgccggagt gctcgaactc cgccttcagc gtgacggcat cggcaagctg
     2221 tcccgcttcg atgcggcgca caatggcctg gtagatgcgg ccatggatcg ggtcggcgaa
     2281 gtgctctggg gagaggaatt ccgagacgcg ctcataggcc cggttgtttg ccagcaatgc
     2341 tcc
//
LOCUS       DATGEM010000442         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_939552, whole genome shotgun sequence.
ACCESSION   DATGEM010000442 DATGEM010000000
VERSION     DATGEM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939552"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VKQ27_14125"
     CDS             <1..207
                     /locus_tag="VKQ27_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY90115.1"
                     /translation="GGRPAKSGNVTVEKAKGGSQAMRKAKGYALEAGDLVTLETGGGG
                     GYGDPSRRAATRIADDIADGYITA"
     gene            complement(192..1544)
                     /locus_tag="VKQ27_14130"
     CDS             complement(192..1544)
                     /locus_tag="VKQ27_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLY90116.1"
                     /translation="MNDHTPTSLLDSDKRHLIHPLHHPNEHAHAHIFVEGKGAILRTA
                     DGTEYIDGLSALWNVNVGHGRKELADAAAEQMAKLAYASAYTGFSNEPAIRLAERIVN
                     LCYGNMAAAYFTTSGAESNESAFKIARYYWKRMGKPNKVKIISRQYGYHGVTLAAMSA
                     TSLPGYHKMFGPLVPEFLQVAPPYQYRWPGNGDAGIGAADSVEAAIEEQGADTIAAVI
                     AEPVMGAGGVIVPPPTYFPRLREICDRHNVLLIADEVITGFGRTGRWFALGHWGVQPD
                     LMSFAKGVTSGYLPLGGVVVSKPVHQAMEDAPMDERFMHAATYSGHPACCAVGLRNID
                     ILEREALPERAAVMGKKLLTSLETLRSLPIVGDVRGLGMMCGVELVEDQGSRKPAIGP
                     GGRVVAEAMKRGLIIRQRGGGGTPVSGDSLCIAPPLMTPEATLDRLVNIVGESIQAVM
                     "
     gene            complement(1687..3165)
                     /locus_tag="VKQ27_14135"
     CDS             complement(1687..3165)
                     /locus_tag="VKQ27_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLY90117.1"
                     /translation="MSDSNANPYLALGVRPFINCCSVRTMHGGSLMLPQVRAAVDAAS
                     RQFVNLDELMEAAGARLAELTGAEWGIVTCGSAAAVALASAACVAGNDPVRMLRLPFT
                     DGWVNRVILPKNQRFAYDQAIRMVGAHIVEIDSLADLDAALAEPVAMVAILGTNEEFS
                     NVRLEQIAARVKPLGIPILVDAASEHLQRPSPWLVRGADMVVYSGGKFLRGPQTSGLL
                     LGKKNLVQAAWRNASPHQAFGRPMKVSKEDVIGVLTAVDYWFNQRDEATELRKWHEDL
                     QEIARRVAAVPSVSTEVIEPKGVVRVPLLSVKWGGIALDGIALRQRLLEGTPRIMLDD
                     TSAQANAINIDPFQLSPGEAAQVGSAIANTLTAAHNLHPSADPAATADVSGEWEVLVQ
                     FLHGKRKHRLKLRQQDSALSGSQSSEQFESNVVGKLAEQRVHMEFETRHEGSAIAYRF
                     EGTVKNGTMQGDVFLGSATDNHRGPVNLSQFGRGQWQASRSA"
     gene            complement(3165..>3540)
                     /locus_tag="VKQ27_14140"
     CDS             complement(3165..>3540)
                     /locus_tag="VKQ27_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLY90118.1"
                     /translation="RGALNGTRILAPETVALMAQNHIGDLTVQKLPTTSPEVSNDAEF
                     FPGMPKKWGLTWLINTQDVPGRRSAGSLAWAGMFNTYYWLDPKKQVTGLLMTQILPFA
                     DPTVLHLFDAFEAEVYRTVGDA"
BASE COUNT          608 a         1120 c         1165 g          647 t
ORIGIN      
        1 ggcggccggc cggcgaaatc cggcaatgtg acggtcgaga aggcgaaggg cggcagccag
       61 gcgatgcgca aggccaaagg ctatgcgctc gaggccggtg atctcgtcac gctggagacc
      121 ggcggcggcg gtggctatgg cgatccgtcg cgccgagcgg cgactcgcat agcggacgat
      181 atcgcagacg gctacatcac ggcctgaatc gattcaccga cgatattcac gagacggtcg
      241 agcgttgcct ccggggtcat cagcggcggc gcgatgcaca gcgaatcgcc gctgaccggc
      301 gtgccgccgc cgccacgctg acggatgatc aggccacgct tcatcgcctc cgcgaccacg
      361 cggccgcccg ggccgatggc aggcttgcgg ctgccctgat cctccaccag ttcgacgcca
      421 cacatcatgc ccaggccgcg cacgtcgcca acgataggca ggctgcgcag cgtctcaagg
      481 ctggtgagca acttcttgcc catgacagcg gcgcgttcgg gcagggcctc gcgctccagg
      541 atgtcgatgt tgcgcaggcc gacggcacag catgccggat ggccggaata agtggccgcg
      601 tgcatgaagc gttcgtccat cggcgcatct tccattgcct ggtgcacggg cttggagacg
      661 acaacgccgc cgagcggaag atagccgctg gtgacgccct tcgcgaagga catcaggtcg
      721 ggctgcacgc cccaatggcc aagcgcgaac caacggccgg tccggccaaa gccggtgatc
      781 acctcgtcgg cgatcagcag cacgttatga cgatcgcaga tctcgcgcag gcgcgggaag
      841 taggtgggcg gcgggacgat cacaccgccg gcgcccatca cgggttcggc aatcaccgcg
      901 gcgatggtat ccgcgccttg ctcctcgatc gcggcttcaa ccgagtcggc tgcaccgatg
      961 ccggcatcgc cattgccggg ccagcgatac tgatagggcg gcgcgacctg gaggaattcc
     1021 ggcaccagcg gaccgaacat cttgtgatag ccaggcaggc tggtggcgct catcgcggcg
     1081 agggtgacgc cgtgatagcc gtactgccgc gagattattt tcactttgtt cggcttgccc
     1141 atccgcttcc aatagtaacg ggcgatcttg aaggcgcttt cgttggattc ggcaccgctg
     1201 gtggtgaaat aggccgccgc catgttgccg tagcacaggt tgacgatgcg ctccgccagg
     1261 cggatcgctg gttcgttgga gaagccagtg taggcggatg cataggcaag cttcgccatc
     1321 tgctcggctg cggcgtcggc gagttccttg cgaccgtggc cgacgttgac attccacagg
     1381 gctgacaggc cgtcgatgta ctcggtgccg tcggcggtgc ggaggatcgc gcctttgccc
     1441 tcaacgaaga tgtgcgcgtg ggcgtgctca ttggggtggt gcagcggatg gatcaggtgc
     1501 cgcttgtcgg aatccagcag cgaggtcggc gtgtgatcgt tcatgatgct tgcctttcgc
     1561 tgttgtcgag gttgctacca ttggtcacga aagcaacgtc gtaccgttag cgattgatgg
     1621 ccgggtcaag cccggccatg acggtggagg ccaatcggcg cggtcgggac gatggggacg
     1681 atagtatcat gccgaacgcg aggcttgcca ttgacccctg ccgaactggc tgaggttcac
     1741 cgggccacga tgattgtcgg tggcgctgcc gagaaacacg tccccctgca ttgtgccgtt
     1801 cttcaccgtg ccctcgaacc gataggcgat ggcgctgcct tcgtgtcgcg tctcgaactc
     1861 catgtggacg cgctgctcgg ccagcttgcc gaccacgttg ctttcgaact gttcggagct
     1921 ctggctgccg ctgagtgcgc tgtcctgctg ccgcagcttc aggcggtgct tgcgcttgcc
     1981 gtggaggaac tgcaccagca cctcccattc accggagaca tccgccgtgg ccgcaggatc
     2041 ggccgagggg tgaaggttgt gagcggcggt cagcgtgttg gcgatggccg agccaacctg
     2101 agcggcctcg ccaggcgaga gctggaaagg atcgatattg attgcattcg cctgcgccga
     2161 agtatcgtca agcataatgc gcggcgtgcc ctccagcagc cgctgccgca gggctatccc
     2221 gtccagcgcg atgccgcccc atttcacgga cagcaaagga acacggacca cgccttttgg
     2281 ctcgatcact tccgtgctga cgctgggcac cgctgcaacg cggcgggcga tctcctgcag
     2341 atcttcgtgc catttgcgca gttcggtagc ctcgtcgcgc tggttgaacc agtaatcgac
     2401 cgcagtcagc acgccgatca cgtcttcctt cgacaccttc atcggtcgtc cgaatgcttg
     2461 gtgcggcgag gcattgcgcc acgcagcctg caccaggttc ttcttgccca gcagcaatcc
     2521 gctggtctgc gggccgcgga ggaatttgcc gccgctgtag accaccatat cggcaccgcg
     2581 caccagccat gggctgggcc gctgcaagtg ctccgaggcc gcgtccacca ggatggggat
     2641 gccgagcggc ttcacccgcg cagcaatttg ctcgaggcgc acgttggaga attcctcgtt
     2701 ggtgccgagg atcgccacca tcgctaccgg ttcggccagc gccgcgtcca ggtcggccag
     2761 cgagtcgatc tcgacgatat gcgcgccgac cattcggatg gcctggtcgt aggcgaagcg
     2821 ctggttcttc ggcaggatta cgcggtttac ccagccatcg gtgaacggca ggcgcaacat
     2881 gcgcaccggg tcgttgccgg cgacacaggc agccgacgcg agtgccaccg cggcagcgct
     2941 gccgcaggtc acgatgcccc attcagcgcc ggtcagttcg gcgagccgcg cgcctgcggc
     3001 ctccatcagt tcatccaggt tgacgaactg gcgcgacgcc gcatccaccg cggcgcggac
     3061 ctgcggcagc atcaggctgc cgccatgcat cgtgcgaacg ctgcagcagt tgatgaacgg
     3121 ccgaacgccg agcgcgaggt aagggttggc gttgctgtcg ctcatcatgc gtctcccaca
     3181 gtccggtaaa cctcagcctc gaatgcatcg aacagatgca gcaccgttgg atcggcaaat
     3241 ggcaggattt gcgtcatcag aagcccggtg acctgcttct tcgggtccag ccagtaatag
     3301 gtgttgaaca tgccggccca ggcgaggctg ccggcactgc ggcggccggg cacgtcctgc
     3361 gtattgatca gccatgtcag gccccatttc ttcggcatgc cggggaagaa ttctgcatcg
     3421 ttggagacct cggggctggt cgtcggcaac ttctgcaccg tgagatcgcc gatgtggttc
     3481 tgcgccatca acgccacggt ttccggcgcc agaatgcggg tcccgttcaa tgcaccacgg
//
LOCUS       DATGEM010000443         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_343145, whole genome shotgun sequence.
ACCESSION   DATGEM010000443 DATGEM010000000
VERSION     DATGEM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343145"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VKQ27_14145"
     CDS             complement(<1..590)
                     /locus_tag="VKQ27_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLY90119.1"
                     /translation="MPIINRIAEFHREMTEWRQDLHTHPELALQETRTSGVVQSKLKE
                     FGVDDIITGIARTGVVGVIRGNGDSTRAVGLRADMDALPILETTGLPYASQNTGVMHA
                     CGHDGHTTMLLGAAKYLAETRNFDGTVYVIFQPAEENYAGGDIMVKEGLFEQCPMEMV
                     FGMHNWPQIPAGTFAWRDGPIMAAVANIEITITGKGA"
     gene            complement(593..1438)
                     /locus_tag="VKQ27_14150"
     CDS             complement(593..1438)
                     /locus_tag="VKQ27_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY90120.1"
                     /translation="MVEPVQVHIIGASGRSGAALSRALQADGIAIVPIVRNAAKLPAT
                     RIADLTDPPALHAALGDATHIVSTAHARFAAAVIAAAPLDARLIFLGSTRKFTHWPDA
                     HGAGVLEGEAAFLASAHHGVMLHPTMIYGAQGEDNVQRLANLLRRLPIVPLPGGGRAL
                     VQPIHQDDVTRAIRAALAHPWHGPQSLVIAGPTALPYAGFVRAIAAAAGLRPPRIVSL
                     PASPLMALASILRYIPLLPRIRPEEIRRLLEDKAFDIRPMVDTLGFTPMPLTEGLART
                     FTPRS"
     gene            1476..3116
                     /locus_tag="VKQ27_14155"
     CDS             1476..3116
                     /locus_tag="VKQ27_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY90121.1"
                     /translation="MADVDPVTVSVVQHRLHAIVEEMGEAMLRTSYSQILNSSRDFST
                     AICDLGGRLIAQAEHVPIHVGALPWAAKSVAEFFGDDIHPGDVFLLNDPYHGGNHLPD
                     LTAFVPIFRDAKPMFWAINRAHQSDIGGSTHGAYNAGATEIWQEGIRVTPLRLYDRGV
                     VRRDVLEMLVTNVRHPHDFRGDLAAMIGSAHAGQRRMLALAGEFGWDTTLAAIEAVLD
                     GAERQARAVIAAWKDGVYHGEAFLDDDGHRYNDIYIRATVTKRGSDLTIDLSDSHEQV
                     VGFVNSSYPNMRSAVVVALAYLIDPHTPKNDGAFRPVEVIAKPGTVVWANEGAPVTLC
                     TNHCSQEILEAIIKALAPACPDRAMAGWGRRFRIAIQGRDPRTQKPFIWHMFQARPGG
                     GASPVGDGWPGGGEWQAAGGIKFGSLEVTEVRFPLFFREHEFRPGSGGDGQYRGGPGC
                     VLEMVAEIDEPARANTAGDGVKYGACGILGGKDGLPHRYVLHSEGRQQRVLKTKEVGI
                     EVRPDDVFRVESGGGGGWGDPAKRDAAARDLDATNGFV"
     gene            3120..>5031
                     /locus_tag="VKQ27_14160"
     CDS             3120..>5031
                     /locus_tag="VKQ27_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY90122.1"
                     /translation="MLKIGIDVGGTFTDLVAVDETGHSTLAKVASTPEDPSVGVMAGL
                     DRLSELLGMPREALLSQTERIVHGTTVATNALLEHKGAKVGLLTTEGHRDVIEMREGL
                     KEDRYNLRMPPPVQLVPRALRLGVKERMRADGRVEVALDRGSLDAAIAELRRAKVEAV
                     AVCYLHAYRDPRHERETAAVLAKAVPDVYVSLSSDVFPQIKEFDRVSTTVVNAYVGPA
                     LSRYLHRLEVRLKEAGYRGPTLIIQSHGGVAPIAESARLAAGGVLSGPAGGVAGSRYA
                     ATLLGSDNLIPFDMGGTSTDICLIVDGEPALVSGRGVGGHRLALNSLDIVSLGAGGGS
                     IGRVDTGGILHVGPESAGASPGPACYGRGGTLPTVTDANLALGYLDPAHFLGGASRLD
                     AAAAERALDRLAAELKVDRVAAAEGVHRVVNTNMAEGIRLVSVRRGVDPRKFALLSFG
                     GAAGLHVTDVARQLDLTRVVVPRVAAVLSAWGMLATDLRFEVSRTHIGDTGRLDGAEI
                     KRLFTEMEAEGLARLRGSFDGPARVQRSVEMRYGEQIFEITVPLDGIDWTVDDPLPEI
                     VQLFHDRHEALYTYALREQDAVLVNARAAVIGVLPGVPHEPVRATAADAAPASVRRIW
                     LRGWTDVPVFDFE"
BASE COUNT          743 a         1332 c         2044 g          912 t
ORIGIN      
        1 gcgcccttgc cggtgatggt gatctcgatg ttggcgacgg cggccatgat cgggccgtcg
       61 cgccaggcga aggtgccggc cgggatctgc ggccagttgt gcatgccgaa caccatctcc
      121 atcgggcact gctcgaacag gccctccttc accatgatgt cgccgccggc gtaattttcc
      181 tccgccggct ggaagatcac atacacggtg ccgtcgaagt tgcgggtctc cgccaggtat
      241 tttgcggctc cgagcagcat ggtagtgtgg ccgtcgtggc cgcacgcgtg catcacgccg
      301 gtgttctgcg aggcgtaggg caggccggtt gtctcgagga tcggcagggc gtccatgtcg
      361 gcgcgcaggc cgacggcgcg ggtggaatcg ccattgccgc ggatgacgcc gacgacgccg
      421 gtgcgggcga tgccggtgat gatatcgtcg acgccgaact ccttcagctt tgactgaacg
      481 acgccgctgg tgcgggtctc ctgcagcgcg agttcgggat gggtgtggag gtcctggcgc
      541 cattccgtca tctcgcgatg gaactcggcg atgcgattga tgatgggcat gctcagctcc
      601 tgggggtgaa ggtgcgcgcg aggccctcgg tgaggggcat gggtgtaaag ccgagtgtat
      661 ctaccatggg gcggatgtcg aaggccttgt cttccagcag ccggcggatc tcttccgggc
      721 ggatccgtgg cagcaacggg atgtatcgca gaatggatgc cagcgccatc aggggtgaag
      781 cggggaggct gacgatgcgg ggtgggcgga ggccagccgc ggcggcgatg gcgcgtacga
      841 agccggcgta gggcagggcg gtggggccgg cgatcaccag cgattgcggg ccgtgccagg
      901 ggtgcgccag agcggcgcgg atggcgcggg tgacgtcgtc ctggtggatg ggctgcacga
      961 gggcacggcc gccgcctggc agcgggacga tggggaggcg gcggaggaga ttggcgaggc
     1021 gctggacgtt gtcctcgcct tgcgcgccgt agatcatcgt ggggtggagc atcacgccgt
     1081 ggtgggcgga ggcgaggaag gcggcttctc cttcgagcac gccggcgccg tgggcgtcgg
     1141 gccagtgcgt gaacttgcgg gtgctgccga ggaagatcag gcgcgcatcc agtggcgcgg
     1201 cggcgatcac cgcggcggcg aagcgtgcgt gcgcggtgga cacgatgtgg gtggcgtctc
     1261 cgagggccgc gtgcagcgct ggcggatcgg tcagatcggc gatgcgggtg gccggcagtt
     1321 tcgcggcgtt gcgcacgatt gggacgatgg cgatgccgtc ggcctggagc gcgcggctga
     1381 gcgcggcgcc gctgcggccc gaggcgccga tgatgtggac ctggaccggt tcgaccatgc
     1441 tgttaccctg ccggtgaaag cagcaggaga ccagcgtggc cgacgtcgac ccggtgactg
     1501 tttcggtggt gcagcatcgg ctgcacgcca tcgtcgaaga gatgggcgag gcgatgcttc
     1561 ggacttcgta ttcgcagatc ctcaattcca gccgcgattt ttccactgcg atctgcgacc
     1621 tgggcgggcg gctgatcgcg caggcggagc atgtgccgat ccatgtgggg gcgttgccgt
     1681 gggcggcgaa gtcggtggcg gagttcttcg gcgacgatat ccatccgggc gacgtgttcc
     1741 tgctgaacga tccgtatcat ggcgggaacc atcttccgga cctgacggcg ttcgtgccga
     1801 tcttccgcga tgcgaagccg atgttctggg cgatcaaccg ggcgcatcag agcgacatcg
     1861 gcgggtccac gcatggcgcg tacaacgcgg gggcgacgga gatctggcag gaagggatcc
     1921 gggtgacgcc gctgcggctg tacgaccgcg gcgtggtgcg gcgcgatgtg ctggaaatgc
     1981 tggtgacgaa tgtgcggcat ccgcacgatt tccgtggcga cctggcggcg atgatcggtt
     2041 cggcacatgc cgggcaacgc cggatgctgg cgctggcggg cgagttcggc tgggacacga
     2101 cgctggcggc gatcgaggcg gtgctggatg gtgcggaacg ccaggcgcgc gcggtgatcg
     2161 cggcgtggaa ggatggcgtg tatcacggcg aggcgttcct ggacgacgac gggcatcgct
     2221 acaatgatat atacatccgc gcgacggtga ccaagcgggg cagtgatctt acgatcgatc
     2281 tgtcggattc gcatgagcag gtggtggggt ttgtcaactc atcgtatccg aatatgcgtt
     2341 cggctgtggt ggtggcgctg gcgtatctga tcgatccgca tacgccgaag aatgatggcg
     2401 cgttccggcc ggtggaggtg atcgccaagc ctggcactgt ggtgtgggcc aatgaaggcg
     2461 cgccggtgac gctgtgcacc aatcactgct cgcaggagat cctggaggcg atcatcaagg
     2521 cgctggcacc ggcgtgtccg gatcgggcga tggcggggtg ggggcggcgg ttccgaatcg
     2581 cgatccaggg gcgcgatccg cgcacgcaga agccgttcat ctggcacatg ttccaggcgc
     2641 ggccaggcgg cggcgcgtcg ccggtgggcg atggatggcc gggaggcggc gaatggcagg
     2701 cggcgggcgg gatcaagttc ggcagcctcg aggtgaccga ggtgcggttt ccgctgttct
     2761 tccgcgagca tgagtttcgg cctgggtccg gtggcgacgg ccaatatcgc ggtgggccgg
     2821 gatgcgtgct ggagatggtg gcggagatcg atgagccggc gcgcgccaat actgcgggcg
     2881 atggggtgaa gtatggcgcg tgtgggatat tgggtgggaa ggacgggctg ccgcaccggt
     2941 atgtgctgca ttcggagggg cggcagcagc gcgtgctgaa gacgaaggag gtggggatcg
     3001 aggtacggcc ggacgatgtg ttccgggttg aatccggtgg cggtggggga tggggcgatc
     3061 cggcgaagcg cgatgcggcg gcgcgcgatc tcgacgcgac caacgggttc gtctgagcca
     3121 tgctgaagat cgggatcgac gtcggtggca cgttcaccga cctggtggcg gtcgacgaga
     3181 ccggacattc cacgttggcg aaggtggctt ccacgccaga ggatccgtcg gttggcgtga
     3241 tggcggggct ggatcggctg tcggagctgc tggggatgcc gcgcgaggcg ttgctttcgc
     3301 agaccgagcg gatcgtgcat ggcacgacgg tggcgaccaa tgcgctgctc gagcacaagg
     3361 gggcgaaggt cgggttgctg acgaccgaag ggcatcgcga cgtcatcgag atgcgcgagg
     3421 gtctgaagga ggatcgctac aatctgcgga tgccgccgcc ggtgcagttg gtgcctcggg
     3481 cgctgcggtt gggtgtgaag gagcggatgc gcgcggacgg gcgcgtggag gttgcgctgg
     3541 atcgtggatc gctggatgcg gcgatcgctg aactgcggcg cgcgaaggtc gaggcggtgg
     3601 cggtctgcta tctgcatgcg tatcgcgatc cccggcatga gcgcgagacg gcggcggtcc
     3661 tggccaaggc agtgccggat gtctacgtgt cgctgtcgtc ggatgtgttt ccgcagatca
     3721 aagagtttga tcgcgtatct acgactgtgg tgaatgccta tgtcgggccg gcgctgtcgc
     3781 ggtatctgca tcgattggag gtgcggctga aggaggccgg gtatcggggg cctacgctga
     3841 tcatccagtc gcatggcggc gtggcgccga tcgcggaatc ggcgcggctc gcggcgggag
     3901 gtgtgctgtc ggggccggcg ggtggtgtgg caggtagccg gtatgcggcg acgctgcttg
     3961 ggagcgataa tctcattcca ttcgatatgg gcgggacgag cactgacatc tgcctgattg
     4021 tcgatggtga gccggcgctg gtgagtgggc gcggcgttgg cgggcatcgg ctggcgctga
     4081 acagtctgga cattgtcagc ctgggcgcgg gcggtggatc gatcgggcgc gtcgatacgg
     4141 gcggcattct gcatgtcggg ccggaaagcg ctggcgcgtc gccggggccg gcctgctacg
     4201 ggcgcggtgg tacgttgccg acggtgaccg acgccaacct ggcgctgggt tatctcgatc
     4261 cggcgcattt tcttggtggg gcaagccggc tggatgcggc tgccgcggag cgggcgctgg
     4321 accggctggc cgcggagctg aaggtggatc gggttgccgc ggcggagggc gtgcatcggg
     4381 tggtgaacac caacatggcg gagggcatcc gcctggtgtc ggtgcgccgc ggtgtcgatc
     4441 ctcggaagtt cgcgctgctg tcgttcggtg gtgcggcggg gctgcatgtg acggatgtgg
     4501 cgcgacagct cgatctgacg cgcgtggtgg tgccgcgcgt ggcggcggtg ttgtcggcgt
     4561 gggggatgct ggcgaccgat ctgcggttcg aggtctcgcg cacgcatatc ggcgataccg
     4621 ggcgattgga tggtgcggag atcaagcggc tgtttacaga gatggaggcg gaagggttag
     4681 cgcgactgcg gggctcgttc gatgggccgg cgcgggtaca gcgcagcgtc gagatgcgtt
     4741 atggggagca gatattcgaa atcaccgtgc cgctggatgg cattgactgg acggtggatg
     4801 atccgctgcc ggagatcgtg cagctgttcc atgaccggca tgaggcgctg tacacctatg
     4861 cgctgcgtga gcaggatgcg gtgctggtga atgcgcgcgc ggcggtgatc ggggtgttgc
     4921 ctggtgtgcc tcatgagccg gtacgtgcga ctgccgcgga tgcggcgccg gcgagcgtgc
     4981 ggcgcatctg gctacgcggc tggacggatg taccggtgtt cgatttcgag g
//
LOCUS       DATGEM010000444         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1268764, whole genome shotgun sequence.
ACCESSION   DATGEM010000444 DATGEM010000000
VERSION     DATGEM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268764"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..3303)
                     /locus_tag="VKQ27_14165"
     CDS             complement(4..3303)
                     /locus_tag="VKQ27_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90123.1"
                     /translation="MNISAPFIARPVATTLLTIGITLAGLFSFFQLPVSPLPQVDIPT
                     IVVTAALPGASPDTVATSVASPLERHLGQIADVTQMTSSSSVGQARVILQFGLDRDIN
                     GAARDVQAAIVAARADLPTSLRSNPTYRKFNPADAPILILALTSKTMTRGQMYDVASN
                     VLQQRLSQIDGIGQVIIGGAALPAVRVELNPQALFKYGIGLEDIRAALASANAHSPKG
                     AIEVGDKHYQLYTNDQASLAADYKPLIIAYRNGAPVKLSDIADVQDSVENLRNEGVAN
                     GSPAVLVIVFRQPGANIIDAVDAVKKALPQLEAAMPSDINVTIANDRSTTIRASLQDT
                     ESTLVIAVILVTLVVYAFLRNARATLIPSIAVPVSIIGTFFTMYLLGFSLDILSLMAL
                     TVSTGFVVDDAIVVLENVSRHLEAGMPRMQAALHGAREVGFTVISISLSLIAVFIPIL
                     LMGTIIGRFFREFAMTLSLAIVVSLGISLTTIPMLCALFLKPMPTRERRRTLFDRALE
                     FYRRTLDWTLRHGVIVMVIWLITIGLNVWLYIIVPKGLFPEEDTGRLIGSMQADQSTS
                     FQAMQQKLDQMVTIVRNDPAVQNVVGFTGQGSGGAGGQSNTGQVFVALKSISQRGSMD
                     SVMTRLRRKLSVVAGARLFLQPVQDFRTGGRQSNAAYQYTLEGDDSRQLYEWSNKLQD
                     ALQNNPVLLDVNSDQQQKGLETDLTIDRDTASRLGVTASQIDNTLYDAFGQRQVSTIY
                     SALNQYHVIMEVAPRYWQNPETLKDIYVSTSGGNASGTQTSNAVVGTVATSTKTTAAT
                     ASANASAIASDSARNAATNALTNIGKGSASSAPAVSTSKETMIPLAAFASYAPGTTPL
                     AVNHQEQFVATTISFNLAPGKSLSDASREIEQAVQRIKMPASLRGSFQGTAQTFQLFL
                     SNELLLVLTALIAVYIVLGILYESTIHPITILSTLPSAGVGAVLALLLFRTEFSIIAL
                     IGVILLIGIVKKNAIMMIDFALDAERTRHLSPRESIFEASLLRFRPIMMTTFAALLGA
                     LPLAVSFGNGGEIRRPLGISIVGGLIVSQALTLYTTPVLYLYLDRFRHAMRRRWRRAF
                     PGIAEASEPAE"
     gene            complement(3300..>3487)
                     /locus_tag="VKQ27_14170"
     CDS             complement(3300..>3487)
                     /locus_tag="VKQ27_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90124.1"
                     /translation="GSGTGSELRRPLGVAIVGGLIVSQVLTLFTTPVIYLMFDRLAVR
                     LSGHGREQPLAGDNAPP"
BASE COUNT          731 a         1037 c         1057 g          662 t
ORIGIN      
        1 gcttcattcg gccggctccg aggcctcggc gataccgggg aacgcgcggc gccaccgtcg
       61 ccgcatcgca tggcggaagc ggtcaagata cagataaagc accggcgtcg tgtagagggt
      121 cagcgcctgg ctcacgatca gcccaccgac gatggatata ccgagcggcc gcctgatctc
      181 gccaccatta ccaaagctga cggccaacgg cagggcccca agcagcgctg cgaacgtcgt
      241 catcatgata gggcggaagc gcaacagcga cgcctcaaag atcgactcgc gcggtgacag
      301 gtgacgcgtg cgctcggcat ccagtgcgaa gtcgatcatc atgatcgcat tcttcttgac
      361 gatgccaatc agcagaatga cgccgatcag cgcgataatg ctgaattccg tgcgaaacag
      421 cagcagtgca agcacggcac cgacgccggc agacggcaaa gtggacagga tcgtgatggg
      481 atgaatggtg ctctcgtaca aaatgcccag cacaatataa accgcgatta gagccgtaag
      541 caccaacagc agctcgttcg acaggaacag ctggaaggtc tgagccgtgc cttggaaact
      601 accacggaga ctcgccggca tcttgatccg ttgcacggcc tgttcgatct ctcgcgacgc
      661 atcgctgagc gacttgcctg gtgcaaggtt gaacgagata gtcgtcgcaa cgaattgttc
      721 ctgatggttc acggcaagcg gagtcgtacc tggtgcatag gacgcgaatg ccgccagcgg
      781 aatcatcgtc tccttgctgg tggaaaccgc cggagccgag gaggcactgc ccttcccaat
      841 atttgtcaac gcattggtcg ccgcatttcg tgcagagtcc gatgcaatcg ccgaggcatt
      901 cgcgcttgcc gtcgccgctg tcgttttggt cgaggtagca acggtgccaa ccacagcgtt
      961 gctcgtctgt gtgccgctgg cattaccgcc cgatgtactg acgtaaatgt ccttcagcgt
     1021 ctctgggttt tgccagtagc gcggtgccac ttccatgatc acgtgatact ggttcagcgc
     1081 gctgtagatg gtggacacct gacgctgccc gaatgcgtcg tacaaggtgt tgtcgatctg
     1141 gctggcggtc accccgagcc ggctcgcggt gtccctgtcg atcgtcagat ctgtttccaa
     1201 ccccttctgt tgctggtcgg agttcacgtc gagcagcacc ggattgttct gcagagcatc
     1261 ctgcagcttg ttggaccact catagagctg gcgcgaatcg tcgccctcca acgtgtattg
     1321 atacgcggca ttgctttgtc gtccgccggt acggaaatct tgcacgggct gtagaaacag
     1381 ccgagcgcct gcgacaaccg acaatttgcg gcgcagccgc gtcataacag aatccatgct
     1441 gccgcgctgc gagatcgatt tcagcgccac gaagacctgc ccggtgttgg actggccacc
     1501 cgcaccgccg ctgccctgcc cggtgaaccc cacgacattc tgcaccgctg gatcattgcg
     1561 cacgatcgtc accatctgat ccagcttctg ctgcatcgcc tgaaatgacg tgctctggtc
     1621 cgcttgcatc gaaccgatca gccgcccggt atcctcctcg ggaaacagac ccttcggcac
     1681 gatgatataa agccagacgt tcaggccgat cgtgatcagc cagatcacca tgacgatcac
     1741 accgtgacgc agcgtccaat cgagtgtacg gcggtagaac tccaaggcgc ggtcgaacag
     1801 cgtgcgccgt cgttcacgtg ttggcatagg cttaaggaac agcgcgcaaa gcatcgggat
     1861 cgtagtgagc gagatgccca gcgatacgac aattgccaat gacaaggtca tagcgaattc
     1921 gcggaagaag cgtccgatga tggtgcccat cagaagaatg ggtatgaaga ccgcaatcag
     1981 cgacaggctg atcgagatga cggtgaagcc gacctcccgt gcgccgtgca gggcggcctg
     2041 catgcgcggc atgccggctt ccagatggcg cgatacgttc tccaacacga cgatggcatc
     2101 atcgacgaca aagccagtcg agacagtcag tgccatcaac gacaatatgt ccaggctgaa
     2161 tcccagcaga tacattgtga agaatgtgcc gatgatcgag acaggcaccg caatcgacgg
     2221 gatcagcgtg gcgcgcgcgt tacgcagaaa cgcgtagacc accagcgtga ccagaattac
     2281 cgcgatgacc agcgtgctct ccgtgtcctg caacgaggct cggatcgtcg tgctccggtc
     2341 attcgcgatg gtgacgttga tatcgctcgg catggccgcc tcgagctgcg gcagtgcctt
     2401 tttcacggcg tcgacggcat cgatgatgtt ggcgccgggc tgacggaaca caatcaccag
     2461 cactgcaggc gaaccgttcg cgacgccctc gttgcgtagg ttttcgacgg agtcttgcac
     2521 gtccgcaatg tcggatagct tgacaggtgc tccattgcgg taggctatga tcagcggctt
     2581 gtagtccgcg gccaagctgg cctggtcgtt ggtatagagc tgatagtgtt tgtctccaac
     2641 ctcgatcgcc cccttagggc tatgcgcgtt cgcggaagcc agcgcggcgc gaatatcttc
     2701 cagaccgatg ccgtatttga acagcgcctg tgggttcagc tcaacgcgca cagctggcaa
     2761 cgcggcgccg ccgatgatga cctgcccgat gccatcgatc tgtgacagcc gctgttgcaa
     2821 cacattgctg gcaacatcgt acatctgccc acgggtcatc gtcttggagg tcagcgccag
     2881 gatcaggatt ggcgcgtcag ccggattgaa cttgcgatag gttgggttgc tgcgcagtga
     2941 cgtcggcaaa tcggcgcgcg ccgcgacgat cgcggcctgc acatcacggg ccgctccgtt
     3001 gatgtcgcgg tctaaaccga attgcaagat gacgcgggct tggcccacgg agcttgagga
     3061 cgtcatctgc gtgacgtcgg cgatctggcc aagatgccgt tccagcggac tggcgacgct
     3121 ggtcgccacc gtgtcgggac tggcaccagg gagcgctgct gtgaccacaa tcgtggggat
     3181 gtccacttgc ggcagcggcg aaaccggcaa ttggaagaat gagaacaggc cggcaagggt
     3241 gatgccgatg gtcagcaacg tggtggcgac cggtcgtgcg atgaatggag ccgagatgtt
     3301 catggcggag cgttgtcacc cgccaagggc tgctcacgcc cgtgccctga gagacgcacc
     3361 gccagccgat cgaacatcag gtagatcacc ggcgtggtga acagcgtcag cacctggctg
     3421 acaatcaggc cgccgacgat ggccacaccc agcggacgcc gaagctcgga acctgtaccg
     3481 gaaccga
//
LOCUS       DATGEM010000445         5152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1382909, whole genome shotgun sequence.
ACCESSION   DATGEM010000445 DATGEM010000000
VERSION     DATGEM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382909"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VKQ27_14175"
     CDS             <1..1321
                     /locus_tag="VKQ27_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLY90125.1"
                     /translation="ERVAAERVDAGTALAQAREAQAALPDLAASRATVEQARTALSGS
                     RAAEATARAERETLNREHAARVARRAAIATERETWAQRAADAARRHEDLLARREAAEI
                     EHTALREAPSQIAERRAEALNALETAEAAHRTAADRLTAAATVSTEADKTARAAEAAL
                     AGSREDAVRAEGAASQAEVGWAAMAERIIERLGENAAMPDPPAELTPEAEEKARRRLE
                     RLLKEREEMGPVNLRADVEADEVEKQIAAIVAEREEITTAIAKLRGSIGHLNREGRER
                     LAAVFERIDNNFQALFSRMFNGGRAHLALVGSDDPLEAGLEIYAQPPGKKLATLSLLS
                     GGEQALTAMSLIFAVFKCNPAPVCVLDEVDAPLDDANVDRFCTLLEDMVRDTNTRFLV
                     VTHHPLTMARMDRLYGVTMQERGVSRLLSVDLQRAAEMAEPPRLAAE"
     gene            1365..1754
                     /locus_tag="VKQ27_14180"
     CDS             1365..1754
                     /locus_tag="VKQ27_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90126.1"
                     /translation="MDKPFDHYSVEIHASVANGLAAQILCDQNKSFVGRIDFYREQEP
                     PVSYLWTPTGSSDPSQIYLVLAMSIDLLEPVITLLEHGGPWELELWPSGPLTGAATDG
                     YGGVLKTTSDIVVPTAPLNVRASRLTT"
     gene            1795..2601
                     /locus_tag="VKQ27_14185"
     CDS             1795..2601
                     /locus_tag="VKQ27_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLY90127.1"
                     /translation="MEKPEARQSIRFDDGAAYERLMGAWSQLVGQRFLDWLSPDAGQR
                     WLDVGCGNGAFTEQLMRRCAPIEVRGIDPSEGQLAFARTRAGTDGAVFMQGDAMALPF
                     DPGRFDAAVMALVIHFLPDPAKGIREMARVVRPGGLVATYVWDALGDGSPTDPILAEM
                     RAMGLAPAGPPSVHASTTDALYRLWTDAGLDRIASRAITVRRSFDDADDYWEASSGTG
                     VPKAMLDQMEAGAIAELKDRVRARVSTGGQRNITLEARANAIMGHVPKSL"
     gene            complement(2818..4071)
                     /locus_tag="VKQ27_14190"
     CDS             complement(2818..4071)
                     /locus_tag="VKQ27_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY90128.1"
                     /translation="MNAPVNMDHEALARSMPLDKIDVSDPRLYYDDAWRPYFARLRRE
                     DPVHYTPDSPYGPYWAVTKYRDIVQVEVNHKVFSSSDEVGGIMINDAPKGMERTSFIR
                     MDPPEHDDQRREVSSTVNPITLAKMEDLIRTRTNMVLDALPRGETFNWVERVSIELTS
                     MMLATLFDYPIDDKARLIRWSDTFICDINAPDAPVHSEEERFAEQMRFAEHMNAFWEE
                     RAKKPKSFDVISIMAHGEATSKMTLRQRMGILVLFLVGGNDTTRNSMSGGLLGLSRFP
                     DQWQKLREHPELVPSAISEFVRWQTPVIHMRRTALEDAEIGGKPIKAGEKVVIWYISG
                     NRDDEVIERADEVVIDRKRPREHMSFGFGIHRCLGNRLAELQLRVLWEEILGRDLRIE
                     VVDKPVYAYSNFLHAIRSLPVRINA"
     gene            complement(4185..>5152)
                     /locus_tag="VKQ27_14195"
     CDS             complement(4185..>5152)
                     /locus_tag="VKQ27_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY90129.1"
                     /translation="RPSFIQMDEPDHGERRKAVSPVVAPANLARMESVVRERTETVLD
                     NLPRNETFNWVKEVSIELTSRMLATLFDWPLEDRHRLMFWSDVTVCHVDTPDAPVHSE
                     AERYAELEKMAKAMKALWQERAASNAQRFDLISMMAHNDATRDMEFRELLANCSLLIV
                     GGNDTTRNSMSGGVWGFAQYPDEYRKLRENPDLMPSAVSEIIRWHTPVLHLRRTALAD
                     FELGGKTIRKGDKVAMWFVSGNRDDDAIKDPHRLIIDRPRAREHISFGFGVHRCVGNR
                     LAELQLRILWEEILKRDLEIEVMEKPKYLRSNLIHGIRELPVQIH"
BASE COUNT          886 a         1530 c         1769 g          967 t
ORIGIN      
        1 cgagcgcgtc gccgcggagc gtgtcgatgc cgggacggct cttgcacagg ctcgggaggc
       61 tcaggccgca ttgcctgatc tcgccgcgtc gcgcgcaacc gtggaacagg cgcggaccgc
      121 gctttcgggt tcgcgggcgg ccgaagccac cgcgcgcgct gaacgggaga cgctgaaccg
      181 cgagcatgct gcgcgggttg ctcgtcgggc cgccatcgcg acggagcggg agacgtgggc
      241 acaacgagcc gccgatgcgg ctcgccgaca cgaggatctg ctggctcgac gcgaagctgc
      301 cgagatcgag cacacggcgc tgcgcgaggc tccttcgcag attgccgagc ggcgggctga
      361 ggcgctgaat gcactggaaa ctgcagaagc cgcgcaccgg acggcagccg accggctcac
      421 tgccgccgcc accgtttcca ccgaagccga caagacagca cgtgctgcag aagctgcact
      481 ggccgggtcg cgcgaagatg cggtgcgggc cgagggtgcc gcgagccagg cggaggtcgg
      541 ctgggccgcg atggcggagc gcatcatcga acggctcggc gaaaatgccg cgatgcccga
      601 cccgccggcg gaactgacgc cggaggccga ggagaaggcg cggcggcggc tcgagcgcct
      661 gctgaaagag cgggaggaga tggggccggt gaacctccgc gccgatgtcg aggccgacga
      721 ggtggagaag cagatcgccg ccatcgtggc cgagcgtgag gagatcacca ccgcgatcgc
      781 caagctccgc ggctccatcg ggcacctgaa ccgcgaaggg cgcgagcggc tggccgcggt
      841 gttcgagcgc atcgacaaca atttccaagc gctgttcagc cggatgttca atggcgggcg
      901 ggcgcatctg gcattggtgg gctcggatga tccgctggag gcggggctgg agatctatgc
      961 gcagccaccg gggaagaagc tggcgacgct gtcactgctt tcgggcggcg agcaggcgct
     1021 gacagcgatg tcgctgatct tcgcggtgtt caagtgcaat cccgcgccgg tctgcgtgct
     1081 ggacgaggtg gatgcgccgc tcgacgacgc caatgtggac cggttctgca cgctgctcga
     1141 ggatatggtg cgcgatacca acacccggtt cctggtggtg acgcaccatc cgctgaccat
     1201 ggcgcgcatg gaccgactgt acggcgtgac gatgcaggaa cgcggcgtgt cgcggctttt
     1261 gtcggtcgat ttgcagcggg cggcggagat ggcggagccg ccgcggctgg cggcggagta
     1321 gtcacgtcct caggcagttc tgtcctccga tacaggagac gacgatggac aagccgtttg
     1381 atcactactc ggttgagatc cacgcatccg tcgccaatgg tctcgccgcg cagatcctgt
     1441 gcgatcagaa caagtcattt gtcgggcgta tagatttcta tcgcgagcag gagccacccg
     1501 tgtcctattt gtggacgcca accgggagca gtgatccaag ccagatttac ctcgtccttg
     1561 ccatgtcaat cgatctgctg gagccggtca tcacgttgct cgagcatggc ggaccgtggg
     1621 agctggaact ttggccttca ggacctttga ccggcgcagc cacggacggc tatggcggtg
     1681 tgctgaagac gacgagcgac atagtcgtgc cgacggcgcc cctgaatgtg agggcgtctc
     1741 gcctgacgac gtagtgtgtt tgggaagcag ggaattccgc agaggatttc ggcaatggaa
     1801 aagccagagg cgcgccagtc catacggttc gatgatggag cagcctatga gcgcctgatg
     1861 ggcgcctgga gccaactcgt gggacagcgc ttcctcgatt ggctgtcgcc tgacgctggt
     1921 cagcgctggc tcgacgtggg ctgcggcaat ggggctttca ccgagcaact catgcggcgc
     1981 tgcgcgccga tcgaagtccg aggtatcgat ccatctgagg gccagcttgc attcgcgcgc
     2041 acacgggcgg ggacagacgg cgcggtattc atgcaagggg atgccatggc gttgcccttc
     2101 gatcctgggc ggttcgatgc ggctgtcatg gcgctggtga tccactttct gcccgacccg
     2161 gccaaaggta tcagggagat ggcacgggtg gttcggcccg gcggattggt cgctacctat
     2221 gtgtgggatg cgctcggcga cggaagtccc accgacccga tcctggcgga aatgcgcgcc
     2281 atgggtctcg ccccggcggg tccgccgagc gttcatgcct ccacaaccga cgcgctgtat
     2341 cggctatgga cggatgcagg cctggacagg attgcatcgc gcgcgatcac ggtgcgacgt
     2401 agcttcgacg atgcagacga ttattgggaa gcaagcagcg ggacgggtgt tccgaaagcg
     2461 atgctcgatc agatggaagc gggtgcaatc gcggaattga aggaccgggt tcgtgcgcgt
     2521 gtctcgacgg gcggccagag gaatatcaca ttggaggcac gtgcgaacgc gataatgggg
     2581 cacgtgccaa agtcgctgtg agtgggaagc tcgaaccgga cgggtttcgg acatcgcgag
     2641 cgcagccttg agcattgagg ctgagttcca tacgactgcc gccagattgc accgacgtgg
     2701 ctacgaatgt gagggcatcc cgcttatcga cgtagtccgg ctggttgcta tcgttgcgag
     2761 cgtggcgaag caaactcgac gagattgctt cgccacgctc gcaatgacag ggtcatgcta
     2821 tgcgttgata cgcacgggca gcgagcggat ggcgtgcagg aagttcgagt aggcgtagac
     2881 gggcttgtcg acgacctcga tgcgcaggtc gcggccaagg atctcttccc acaggacgcg
     2941 caactgcaat tccgccaggc ggttgcccag gcagcgatgg atgccgaatc cgaacgacat
     3001 gtgttcgcgc ggacgcttgc ggtcgatcac cacttcatcc gcccgctcga tcacttcgtc
     3061 gtctcgattg cctgagatat accagatcac caccttctcg ccggccttga tcggcttgcc
     3121 gccgatctcg gcgtcctcga gcgcggtgcg gcgcatgtgg atcaccggtg tttgccagcg
     3181 gacgaattcc gagatggcgc ttggcacaag ttcgggatgc tcacggagtt tctgccactg
     3241 atcggggaag cggctcagtc ccaacaggcc acccgacatc gagttgcgcg tcgtgtcgtt
     3301 gccgcccacc aggaacagca ccaggatgcc catgcgctgg cgcagcgtca ttttgctggt
     3361 cgcctcgccg tgcgccatga tcgagatcac gtcgaagctc ttcggctttt tcgcccgctc
     3421 ctcccagaac gcattcatgt gttcggcgaa gcgcatctgt tcggcgaagc gctcttcctc
     3481 ggaatgcacc ggcgcgtcgg gtgcgttgat gtcgcagatg aacgtgtcgg accagcggat
     3541 cagtcgcgcc ttgtcgtcga tcggatagtc gaacagcgtc gccagcatca tcgaggtgag
     3601 ctcgatcgat acgcgctcta cccagttgaa tgtctcgccg cgtggcaggg catcgagtac
     3661 catgttggtg cgcgtgcgga tcaggtcttc catcttggcg agggtgatcg ggttgaccgt
     3721 ggagctcacc tcgcgccgct ggtcgtcgtg ctcgggcggg tccatgcgga tgaagctggt
     3781 gcgctccatg cccttcggcg cgtcgttgat catgatgccg ccgacttcgt cggacgagga
     3841 gaacacctta tggttcacct cgacctggac gatgtcgcgg tatttggtga cggcccagta
     3901 ggggccatag ggactgtccg gtgtgtaatg cacggggtct tcgcgccgca gccgcgcgaa
     3961 gtagggccgc caggcatcgt cgtaataaag cctgggatcg ctgacatcga tcttgtcgag
     4021 cggcatcgac cgggccagag cctcgtgatc catgttgacc ggcgcgttca tggcgttcct
     4081 ccttttcttt ccactgcgag gaacgtggcg gtggagcaat ctcgttggcg tggcaggcgc
     4141 gccggtttag cgagattgct tcgctacgct cgcaatgaca tcggctagtg gatctgcacc
     4201 ggcagctcgc ggatgccgtg gatcaggttc gagcgcaggt atttcggttt ttccattacc
     4261 tcgatctcga gatcgcgctt gaggatctcc tcccacagga tgcgcagctg cagctcggcc
     4321 aggcggttgc cgacgcagcg gtgcacgccg aagccgaacg agatgtgctc gcgcgcgcgt
     4381 gggcgatcga tgatcagccg gtgagggtcc ttgatcgcgt cgtcgtcgcg gttgccggag
     4441 acgaaccaca tcgcgacctt gtcgcccttg cgtatcgtct tgccgccgag ttcgaaatcg
     4501 gcgagtgcag tgcggcgcag atgcagcacc ggcgtgtgcc agcggatgat ctcggacacc
     4561 gcgctcggca tcaggtcggg gttctcccgc agcttgcggt attcatcggg gtactgggca
     4621 aagccccata cgccgcccga catcgaattg cgcgtggtgt cgttgccgcc cacgatcagc
     4681 aggctgcagt tcgcgagcag ttcgcggaat tccatgtccc gcgtggcatc gttgtgcgcc
     4741 atcatcgaga tcaggtcgaa gcgctgcgcg ttggaggcgg cgcgttcttg ccacagcgcc
     4801 ttcatcgcct tggccatctt ctccagctcg gcgtagcgct ctgcctcgga atgcaccggc
     4861 gcgtcaggcg tgtcgacgtg gcagacggtg acgtcggacc agaacatcag ccgatgccga
     4921 tcctcgagcg gccagtcgaa cagcgttgcc agcatgcgcg aggtgagttc gatcgagacc
     4981 tccttcaccc agttgaaggt ctcgttgcgc ggcagattgt cgaggacggt ctcggtgcgt
     5041 tcgcggacca cactttccat gcgcgcgagg ttggcgggtg ccacgacagg gcttaccgct
     5101 ttgcggcgct cgccgtggtc gggttcgtcc atctggatga agctcgggcg ct
//
LOCUS       DATGEM010000446         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_483565, whole genome shotgun sequence.
ACCESSION   DATGEM010000446 DATGEM010000000
VERSION     DATGEM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483565"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /locus_tag="VKQ27_14200"
     CDS             complement(<1..1981)
                     /locus_tag="VKQ27_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLY90130.1"
                     /translation="MPGLLGADQAFAAPGNFDPSVWYSIAPDGIVTVTSGKADMGQHI
                     ASTMAQIICEELGASWKDMRVQLASNDPKYNDPVLGAQVTGGSWSTMMNFDAMSRAGA
                     AGRIALTEAAAAMMGGIPAGQLIVRNGTITDPKSKKSMSFADVVKSGKITKTFTADDL
                     KAIKLKTPDQYTMIGVSVPQLDIPSKTNGTAKYGIDVMLPGMVYGKVVTPPVRYGATV
                     KTVDDSEARKVPGFIKAVTLDDKTGTTTGWVVAVANTFAGATKAAAALKVTYDGGPNA
                     KLSSESLLAEARRLQGLDDSGLFFVKDGDTTAALGTAAKVLEAEYTTSINIHAPMEPM
                     TAAAEQKGDIWHVYTGNQFATRSGAIAAGAAGVDPKFVVMHQMWLGGGFGRRLEGDML
                     VPAVQASKAVGKPVKIIYTRENDMTMDFTRPLTFQKIKAGLDGDGKLVAMNHDVVAAW
                     PTKRWGIPAFLSPSVDKKGALDAFTVNGADFFYTVPNHNVRAIINEMAQTATPSGQLR
                     SVAPGWTFWAVESMVDELAHAAGKDPAQFRIDLLDGKGANNGGAQRLRNTLLTAMGLA
                     GYGTRELPKGEGMGVACVSSQERATASWTACVAHVAVTPSGEVKVKKLTVATDIGTQV
                     HPDNVRAQGEGAALWGLSLALYEKATLKNGGIEQTN"
     gene            complement(2086..2577)
                     /locus_tag="VKQ27_14205"
     CDS             complement(2086..2577)
                     /locus_tag="VKQ27_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY90131.1"
                     /translation="MPSLTINGRTQSVDAANDTPLLWVIREQLQMTGTKFGCGAGLCG
                     ACTVHINGEAVRSCQTQVSDAVGKKITTIEGLSAKGDHPLQKAWIAEQVPQCGYCQSG
                     QIMQAASLLAKNANPTKEEVVAHMDGNLCRCMTYSRIQKAIMRAATEMRTASNAGNER
                     RAT"
BASE COUNT          513 a          967 c          997 g          658 t
ORIGIN      
        1 aattggtttg ttcgataccg ccattcttca gcgtcgcctt ttcgtacagc gcaagcgaca
       61 acccccacag cgccgcgcct tcgccctggg cacgaacgtt gtcgggatgc acctgggtgc
      121 cgatatcggt cgccaccgtc agtttcttga ccttgacttc gccgctcggc gtgaccgcca
      181 catgagcaac gcaggcggtc cagctggcgg tggcgcgttc ctgcgacgac acgcaggcca
      241 cgcccatgcc ttcgcccttc ggcaactccc tggtgccgta gccggcgagg cccatggccg
      301 tgagcagcgt gttacgcagc cgctgcgcgc cgccattgtt ggcgcccttg ccgtcgagca
      361 ggtcgatccg gaattgcgcc ggatccttgc ctgcggcatg ggcgagctca tcgaccatgc
      421 tttcgaccgc ccagaacgtc cagcccggcg cgaccgaccg caactggccc gagggggtag
      481 cggtctgtgc catctcgttg atgatggcgc ggacgttgtg gttgggtacg gtgtagaaga
      541 agtccgcacc gttcaccgtg aacgcgtcga gcgcgccctt cttgtcgacc gaaggcgaca
      601 ggaaggccgg aatgccccag cgcttggtcg gccaggccgc gacgacatcg tggttcatcg
      661 ccaccagttt gccgtcgccg tccagtccag ccttgatctt ctggaacgtc agcggccgcg
      721 tgaagtccat cgtcatgtcg ttttcacggg tgtagatgat cttcaccggc ttgccgacgg
      781 ctttcgaggc ctgcacggcc ggcaccagca tgtcgccctc gagacgccgg ccgaagccgc
      841 cgccgagcca catctgatgc atcacgacga acttcggatc gaccccggcc gcacccgctg
      901 caatcgcacc ggagcgtgtc gcaaactggt tgccggtgta gacgtgccag atgtcgccct
      961 tctgctcggc tgccgccgtc atcggctcca tcggcgcgtg gatgttgatg ctggtggtgt
     1021 attcggcctc cagcaccttc gccgccgtcc caagcgccgc ggtcgtgtcg ccgtccttga
     1081 cgaagaacag gccggaatcg tcgaggccct ggaggcgcct tgcttccgcc agcagggatt
     1141 cgctcgacag cttggcgttg gggccgccgt cataggtgac cttgagcgct gcggctgcct
     1201 tggttgcgcc ggcaaacgtg ttcgccaccg caaccaccca tccggtggtc gtgcccgtct
     1261 tgtcgtcgag cgtgaccgcc ttgatgaagc caggcacctt cctggcttcg ctgtcgtcga
     1321 ccgtcttgac cgtggcgcca tagcgcaccg gcggggtgac gaccttgccg tagaccatgc
     1381 ccggcagcat gacgtcgatg ccgtatttcg cggtcccgtt cgtcttcgag gggatgtcga
     1441 gttgcggaac cgaaacaccg atcatggtgt actgatccgg cgtcttcagc ttgatcgcct
     1501 tgagatcgtc agccgtgaaa gtcttggtga tcttgccgct cttgacgacg tcggcgaagc
     1561 tcatcgactt cttcgacttc ggatcggtga tcgtcccgtt tctcacgatc aactggccgg
     1621 ccgggatgcc gcccatcatc gcagcggcag cttccgtcaa cgcgatccgc cccgcggcac
     1681 ccgcgcggct catcgcatcg aagttcatca tggtgctcca gctgccgccg gtgacctgcg
     1741 cgccgagtac aggatcgttg tacttcggat cgttggaggc gagctgaacg cgcatatcct
     1801 tccagctggc gccgagttct tcgcagatga tctgcgccat ggtggaggcg atgtgctggc
     1861 ccatgtcggc cttgccgctg gtgacggtaa cgattccgtc cggcgcgatc gagtaccaca
     1921 cgctgggatc gaaattaccg ggcgcggcaa aagcctggtc ggcgccgagc aggccgggca
     1981 cggctgaata gccgagcgcg agacctgcgg cggccgagcc aacgaggaac gagcggcggc
     2041 tcagatcggc cggctcatga tgggtgatgt ttttcacgtg cgaattcatg tggccctccg
     2101 ctcgttgccg gcgttggagg cagtgcgcat ttccgtagct gcgcgcatga ttgctttctg
     2161 gatgcgcgaa taggtcatgc atcggcagag attgccgtcc atgtgcgcaa cgacttcttc
     2221 cttggtcggg ttggcgttct tcgccagcag cgaagcggcc tgcatgatct ggccggactg
     2281 gcagtaaccg cattgcggca cttgctcggc gatccaagcc ttctgcaacg ggtgatcgcc
     2341 cttggcggac aggccttcga tggtcgtgat cttcttgccg acggcgtcgc tgacttgtgt
     2401 ttggcaggag cgtacggctt caccattgat gtgaacggtg caggccccgc acagtccggc
     2461 gccgcagccg aacttcgtgc ctgtcatttg gagttgttcg cggatgaccc agaggagcgg
     2521 cgtatcgttg gccgcatcga cggactgagt ccgcccattg atggtgagac ttggcatagg
     2581 ttctttccct actcggtgat gaaaaccgct tacggcggaa ctcatttttt cttgttggat
     2641 ggcgcccacc ggaatggcgc cggcaggaag aatgatagcg aaccatcggc gaggcaatga
     2701 aaatccgtcg cggcgaatct caaaccttgg ttgtgcggtg cgacagcgac gctgtcatac
     2761 cgtttcccgg aatatcacgt gtgttggtgg cttgaggtgc cggtaaaacg gccagttgga
     2821 ctgcttcggt tcgctagtgt ggtggttcag aagtccgcat cattcgtgcc gcgcgttcgt
     2881 catgcggact tctgaaccaa agccacacta gatcaatagg tttgctagtg tcctttcgat
     2941 tccgaagttc gaaacgtggc tccgcaaagt gggacgaact tcggaatcgg gacactagaa
     3001 ccagcgttcg ccgacgagaa tggtatcgcc ggggctgatg gcggtgccga gcgggacgat
     3061 gacgcggccg gtgttgtggg tcagcgtgac gctgtcgcgt ttggcgcggg gcgagaagcc
     3121 gccggcaatc gcaac
//
LOCUS       DATGEM010000447         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1606241, whole genome shotgun sequence.
ACCESSION   DATGEM010000447 DATGEM010000000
VERSION     DATGEM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606241"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..660
                     /locus_tag="VKQ27_14210"
     CDS             121..660
                     /locus_tag="VKQ27_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY90132.1"
                     /translation="MTAALRILLRTDDPVRQQGLEAMLQAAGHTVVDDTPDVVLCDID
                     HDMATPPEAEAPVVALNDHPHPGEPSAGVLPRTVSTTQLDAALRAVAAGLLVRWPGAA
                     SADGFRPAREDAPLLTPREAEILALVGQGMSNKEAARALGISVHTVKFHLEALFTKLD
                     ATSRAEAVAKGLRGGVIEL"
     gene            complement(673..1965)
                     /locus_tag="VKQ27_14215"
     CDS             complement(673..1965)
                     /locus_tag="VKQ27_14215"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLY90133.1"
                     /translation="MTHPLSVAVAGLGTVGAGVMKLLRDNADIVAARAGRPVAVTAVS
                     ARDRTRDRGVSLTGLRWYEDAVALAADPAVDVIVELIGGSDGPARALVQAALAAGKPV
                     VTANKALLAVHGAELAAAAEEGGVPLAFEAAVAGGIPVIKALREGLAGNRIGRIAGIL
                     NGTCNYILTQMRERGREFAEVLADAQKLGYAEADPSFDIDGIDAAHKLAILAALAFGR
                     PVAFDAVHVEGIRKVSALDIAFASELGYRIKLLGIARQGENGIETRVHPCMVVQTAPI
                     ARVDGVFNAVVAEGDFVGRVMLEGRGAGAGPTASAVVADLVDIARNRVTPVWGAGSAA
                     LSNAPSVPMSEHVGAYYLRLMVVDRPGVIADVTAVLRDLGVSLESMLQRGRAPGEAVP
                     VVLVTHETQESAMREALSRIADLNAVLEAPTMIRIEPA"
     gene            complement(2645..>3154)
                     /locus_tag="VKQ27_14220"
     CDS             complement(2645..>3154)
                     /locus_tag="VKQ27_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY90134.1"
                     /translation="LSKTYSMPGWRMGFAAGNPRLINALTRMKSYLDYGAFTPIQVAA
                     AAALNGPQDCVDQIRTLYRERRDVLIRGLASAGWDVPSPDGSMFAWAPIPARFSGLGS
                     VEFSKLLLARAKVAVSPGLGFGEHGEGYVRIALVENTHRLRQAVRNIRAFLQAGTNLA
                     EPEVVTAVP"
BASE COUNT          604 a         1171 c          886 g          493 t
ORIGIN      
        1 tgctgcgggc cggcgacatc cgtctggtca ccgtgatcat cggcacccaa ccggcgaagg
       61 aatgatggcc ggatcaagtc ccatgacagg tgaagatcgg gcactcgccc gcactcttgc
      121 atgaccgcag ccctgcgcat cctgctgcgc accgacgacc cggtgcggca gcagggcctg
      181 gaggcgatgc tgcaggccgc cggccacacc gtcgtcgacg acacacccga cgtcgtcctg
      241 tgcgatatcg accacgatat ggccacacca cccgaggccg aagctcccgt ggttgcccta
      301 aacgatcacc ctcaccctgg cgaaccttcc gccggtgtcc tccctcgcac tgtcagcacc
      361 acacaactcg acgccgcctt gagagcagtc gccgccggcc tgctcgtccg ctggcccgga
      421 gctgcatcag ccgatggctt ccgccctgca cgcgaagatg caccgctcct cactccgcgc
      481 gaagcagaga tccttgccct ggtcgggcaa ggcatgagca acaaggaagc cgcccgagcg
      541 ctcggcatct cggtccacac ggtcaaattc cacctggagg ctctgtttac caagctcgac
      601 gccaccagcc gggccgaagc cgtcgccaaa ggcctccgcg gtggtgttat cgagctctga
      661 tccctgcgcc gtttacgccg gttcgatccg tatcattgtc ggcgcctcca gcaccgcgtt
      721 gaggtcggcg atccgcgaca gcgcctcgcg catcgcgctc tcctgcgtct cgtgcgtaac
      781 cagcaccacc ggcaccgcct cacccggtgc tcgcccacgc tgcagcatcg attccagcga
      841 cacgcccaaa tcgcgcagca cggccgtgac atcggcgatc acccctggcc gatccaccac
      901 catcagccgc aggtaatacg cgcccacatg ctcgctcatc ggcaccgaag gtgcgttgga
      961 gagtgctgcc gaacctgccc cccaaaccgg ggtcacacgg ttgcgcgcga tgtcgacgag
     1021 gtcggccacg accgccgacg ccgtcggtcc agcgcccgcg ccgcgccctt ccagcatcac
     1081 gcgcccgacg aagtcgccct cggccacaac ggcattgaac acgccatcca cccgagcgat
     1141 cggcgccgtc tgcaccacca tgcagggatg cacacgcgtc tcgatcccat tctcgccctg
     1201 ccgcgcgatc cccagcagct tgatccggta gccaagctca ctggcaaacg cgatgtcgag
     1261 cgcggatacc ttccggatgc cctccacatg caccgcgtcg aacgccaccg ggcgcccgaa
     1321 cgcgagcgcc gccaggatag ccagcttgtg cgccgcatcg atcccgtcga tgtcgaacga
     1381 cggatcagcc tcggcatagc ccagcttctg cgcatcggcg agcacctcgg cgaactcccg
     1441 acctcgctcg cgcatctgcg tcaggatgta gttgcaggtg ccattcagga tgcccgcgat
     1501 ccggccaatg cggttgcctg ccaagccctc gcgcagcgcc ttgatcactg gaatgccgcc
     1561 ggccacagcc gcctcgaacg ccagcggcac gccaccttcc tctgctgctg cggcgagctc
     1621 cgcgccatgc acagcaagca gcgccttatt cgcggtcacc actggcttgc cagccgccaa
     1681 cgccgcttga accagcgcac gcgcgggccc gtctgagccg ccgatcaact ccacaatcac
     1741 atccaccgcc ggatcggccg cgagcgccac cgcatcctcg taccagcgca gccctgtcaa
     1801 cgacacgccg cgatcacgcg tacgatcacg cgctgataca gctgtaactg cgacgggacg
     1861 cccggcgcgc gctgcaacaa tatcagcgtt atcgcgcagc agcttcatca cgccagcgcc
     1921 aacggtccct agaccagcga cagcaacgga aagaggatgt gtcatgagcg cggactccgg
     1981 ccctgcaatg cgaggcgagc ggacattatg gatgacgtcc ttttggtgag tagtgacggc
     2041 tgcatagcag actggatcaa gcaatgcagg tcctatgagc gagagaggcc cgaatggaag
     2101 gtcgtgcaga cagacggcct acacgtccga atggctgagc cagccggtct cgagtagtct
     2161 cctcccacgc cagatgatct ctacgaccat gacgccctcg cctggtcgga acatcaggcc
     2221 agcctgctac ggcgcgtcgc acggggcgag cgcgtgaacg aggtcgatcg ggaacatatc
     2281 acccgggaga tcgaagatgt gggtctcgca actcaatggt gttcagagct ttcttacgcc
     2341 tgacgctggt tcaactgctc aaacttcatt gttggccgga gagtgcctca acaggccatt
     2401 ggcgaagaga aatcgtttcg tttcaaaacg acgccgctca gcggttcgca ccgtcaatgc
     2461 gccagaggat cgacctgaac cggctctgcg acgcggccaa ggaccagata tcagcagcca
     2521 gccatgacgg gcagccagcc ctgccattgc cgcccgactg ccccttcacg cttgatcaac
     2581 tcctggcggg aacggggcga tgcactacag gctcagctct ccgctcaccg cgttggcaac
     2641 aagctcacgg caccgcggta accacctctg gctctgccag gttcgtcccc gcctgcagaa
     2701 acgctcgtat attgcgcacc gcctgccgca agcggtgcgt attctccacc agcgcgatcc
     2761 gcacgtaccc ctcgccgtgc tcgccgaacc ccaagcctgg cgacaccgcc accttggccc
     2821 gtgccagcag cagcttggag aattccacgc tcccaagacc cgagaagcgc gccggtatcg
     2881 gcgcccaggc gaacatcgag ccatccgggc ttggcacatc ccagccggcc gaagccaacc
     2941 cgcggatcag cacatcgcgc cgctctcgat acaacgtccg gatctggtcc acgcaatcct
     3001 ggggcccgtt cagcgccgcc gcagccgcca cctggatcgg cgtgaacgca ccataatcca
     3061 agtacgactt catccgcgtc agcgcgttga tcagccgcgg attgcccgcc gcgaacccca
     3121 tccgccagcc cggcatcgag taggtcttcg acag
//
LOCUS       DATGEM010000448         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_14076, whole genome shotgun sequence.
ACCESSION   DATGEM010000448 DATGEM010000000
VERSION     DATGEM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14076"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VKQ27_14225"
     CDS             complement(<1..432)
                     /locus_tag="VKQ27_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90135.1"
                     /translation="MTAGDEPVLIEIAGLTKRFGGFTAVDDVSFRVARGEVLGFLGPN
                     GAGKSTTMRMLAGFMIPTAGVARICGHDVQADGVAARRVLGFLPEGAPTYPEMTVGGF
                     LRFCAKIRGFGRRLLAERVEQAVALTRLESVMLQPVETLSKG"
     gene            617..1396
                     /locus_tag="VKQ27_14230"
     CDS             617..1396
                     /locus_tag="VKQ27_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLY90136.1"
                     /translation="MPLESLETKEVALADAQWQARVDLAAAHRLCVMEGFHEGIFNHL
                     TLRVPGYNDRYYQIPFGLHWSEVTASCLMEVGYDGTLLAGEGEVERSAYCIHAPLHRL
                     LPDGAAVFHTHQPYASALARLEDPRILPIGQTELGIMMVTGYDFEYDGPAFDPAEGER
                     LAGVIGDKKVLMMAGHGIATVGRSVAEAYDLLYYSERVAQVQLLAMSSGRPLKVLPEA
                     VIEKTFAQYRKGEKYGGRSPHHWHFDALKRILDKQQPDYKD"
     gene            1488..1697
                     /locus_tag="VKQ27_14235"
     CDS             1488..1697
                     /locus_tag="VKQ27_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90137.1"
                     /translation="MDTAACNLPFLGADAFLRTHEMLARAYRHHDAGWFFESIAGQHG
                     QVVLPSLGITLPLAALYESVVPEGD"
     gene            1709..>2413
                     /locus_tag="VKQ27_14240"
     CDS             1709..>2413
                     /locus_tag="VKQ27_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY90138.1"
                     /translation="MEDPADLPALIAPSERTLPQMLRRQAALHGPRKLLDIGGSVWTF
                     ADTLEMAARFGGTLRAARIERGDHVAIICGNRAELLQVYLGCGWIGAVTVPINVASRG
                     AQLAHILTNSQAKLLVVQAEHAEALETLDAVPSSLCAVWLIGDTPVATRWPNMAKFPA
                     PAEPVPEVPVAPGDTVAILYTSGTTGVSKGVCCPHAQYFWWGVNTARLLGVRGDDVLL
                     TTLPLFHTNALNTFYQA"
BASE COUNT          419 a          824 c          745 g          425 t
ORIGIN      
        1 gcctttcgac agcgtctcta ccggctgcag catcacgctt tccagacggg tcagggccac
       61 tgcctgctcg acgcgctcgg ccagaagcct gcggccgaag ccgcgtatct tcgcgcagaa
      121 cctcaggaac ccgcctacgg tcatctcggg atacgtcggt gctccctcag gcaggaagcc
      181 cagcacccgc cgcgccgcca cgccgtccgc ctgcacgtcg tgtccacaga tccgcgcaac
      241 cccggcggtc gggatcatga aaccggcgag catgcgcatt gtggttgact tgccggcacc
      301 gttcgggccg aggaagccaa gcacctcgcc tcgcgccacg cggaacgaaa cgtcgtccac
      361 tgccgtgaag ccgccgaatc gcttggtcag cccagctatc tcgatcagta caggttcgtc
      421 cccggcggtc acgccgcgcc tcctcacgag tccccgtcgg gcggcgggaa taccggaaat
      481 gcctgccaat gcaagcggct ggcttcgatg tgacgaagcc ttaacccgat cgcaatcgaa
      541 ttatcgagcg gcattgaccg atgctgcact gcggtagatc atccttttcc agcaagaaat
      601 cgaggagccc gtcgtcatgc cgctggagtc cctggaaacg aaggaagttg ctcttgccga
      661 cgcgcaatgg caggcccgcg tggatctcgc ggcggcacac cgcctgtgcg tgatggaggg
      721 cttccacgag ggtatcttca accacctgac ccttcgtgtc cctggctata acgatcgcta
      781 ctaccagatc ccgttcggcc tgcactggtc cgaggtcacc gcgagctgcc tgatggaagt
      841 gggctacgat gggacgctgc tcgccggcga aggcgaggtc gagcgctcgg cctactgcat
      901 ccacgcaccg ctgcatcgcc tgctgccgga cggcgctgcg gtgttccata cgcatcagcc
      961 ctatgccagc gcgctcgcgc gactggagga tccgcggatc ctgccgatcg gccagacgga
     1021 gctcggcatc atgatggtga caggctacga cttcgaatat gacggcccgg cctttgaccc
     1081 ggcggagggc gagcgcctcg ccggcgtgat tggcgacaag aaggtgctga tgatggccgg
     1141 gcatggcatc gccaccgtcg gccgctccgt cgccgaggcc tatgacctgc tctactattc
     1201 cgagcgcgtc gcgcaggtgc agcttctcgc tatgtcgagc ggccgcccgc tgaaggtcct
     1261 gcctgaggcc gtgatcgaga agaccttcgc gcagtatcgg aagggggaga aatacggcgg
     1321 gcgctcgcca caccactggc acttcgatgc actgaagcgg attctcgaca agcagcaacc
     1381 cgactacaag gactgatcca ttgccatctc cctcttcgcc tgggggcgaa gagggagcag
     1441 gttctccgcg cgtggcggat cggccacgcg cgtgatacca tccgatcatg gacacagcgg
     1501 catgcaacct gccattcctg ggcgcggacg cattcctgcg cacgcatgag atgctggcac
     1561 gcgcctatcg tcatcatgat gccggctggt tcttcgaatc gatcgcaggc cagcatggcc
     1621 aggtagtgtt gcccagccta ggcatcaccc tcccgttggc ggcactctat gagagtgtcg
     1681 tccccgaagg cgactgatcc gttccacgat ggaagaccct gctgacctcc cggcactgat
     1741 cgctccgagc gagcgtacgc tgccccaaat gctccgccgt caggcggcgc tccacggtcc
     1801 gcgtaagctg ctcgacattg gcggcagcgt gtggacattt gccgatacac tggagatggc
     1861 cgcccggttc ggaggcacat tgcgtgccgc gaggatcgaa cgaggcgacc atgtcgcgat
     1921 catctgcggc aaccgtgccg agctgctcca ggtctatctc ggctgcggct ggatcggcgc
     1981 cgtcaccgtg ccgatcaacg tcgcgtcccg gggcgcgcag ctcgcgcaca tcctgaccaa
     2041 ctcgcaggca aagctcttgg tcgtgcaggc ggagcacgcc gaggcactgg agacgctcga
     2101 cgctgtcccc tcttcgctgt gtgcggtgtg gctgatcggc gacaccccgg tcgcgacccg
     2161 ctggccgaac atggcgaagt tcccggctcc ggccgaaccc gtccccgaag ttcctgtcgc
     2221 tcccggcgat accgtcgcga tcctctacac gtcgggtacc acgggcgtct ccaaaggtgt
     2281 ctgctgcccg cacgcgcagt acttctggtg gggcgtcaac accgcacgcc tgctcggcgt
     2341 gcgcggcgac gatgtactgc tgaccacgct gccgctgttc cacaccaacg cgctgaacac
     2401 gttctaccag gcg
//
LOCUS       DATGEM010000449        16504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1623115, whole genome shotgun sequence.
ACCESSION   DATGEM010000449 DATGEM010000000
VERSION     DATGEM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16504
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623115"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VKQ27_14245"
     CDS             complement(<1..440)
                     /locus_tag="VKQ27_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90139.1"
                     /translation="MRPADVFHSTTVRWALAVAGAFGVCTVLLFGFVYWQTAMYVTGK
                     VDTIVLANSHFDPSLPQTQALHFVTEHLEDDPRRVKLAELFAPDGAPIAGNVASLPPR
                     LPLGASPRAVELVRVDNRGRELQTARVIARRLASGDILLVGHDIG"
     gene            complement(427..1119)
                     /locus_tag="VKQ27_14250"
     CDS             complement(427..1119)
                     /locus_tag="VKQ27_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008852423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY90140.1"
                     /translation="MRKVAMAKVLVIEDDDGTAEEIVTALDDRGYALDRAADGTEGLQ
                     RARAGGWDVLVVDRMLPGLDGLTVVETLRHEGIKTPVLVLSALNAVNDRVRGLRAGGD
                     DYLAKPFALSELAARLEALLRRPADPRETVLRIGPLELDLLARAAHRGHRELDLLPRE
                     FQLLEYMMRREGQVLTRAMLLENVWNYRFVPQTNLVDVHIGRLRRKVDAPDEPPMILS
                     VRGAGFVLRAPG"
     gene            complement(1129..1926)
                     /gene="truA"
                     /locus_tag="VKQ27_14255"
     CDS             complement(1129..1926)
                     /gene="truA"
                     /locus_tag="VKQ27_14255"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLY90141.1"
                     /translation="MVADPTARARWALLLEYDGGPFVGWQRQATGLSVQEVLENAAEK
                     LNRGKPVSTTVAGRTDAGVHAAEQVVQLELPAAIYAGKVRDALNYHMKPHPVAVLQVA
                     PAPDEWNARFSAVGRVYRYRILNRRARPALLAGRVWHVPAPLDAAAMHAAAQTLLGRH
                     DFTSFRAAACQANSPLRTLDRLDVRRDGEGIEIVAEARSFLHHQVRNMVGTLKLVGDG
                     SWPIERVAAALSARDRSAAGPTAPPDGLCLIAVRYITDLFEKVGLAP"
     gene            complement(1927..2835)
                     /gene="fmt"
                     /locus_tag="VKQ27_14260"
     CDS             complement(1927..2835)
                     /gene="fmt"
                     /locus_tag="VKQ27_14260"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLY90142.1"
                     /translation="MRLAFMGSPDFSVPALRTLHGAGYEIAAVYCQPPKPAGRGYALR
                     PCPVQAAAGALGLPVRTPRRLRTDPATQEAFAALDLDAAVVAAYGLILPPAMLAAPRR
                     GCLNIHASLLPRWRGAAPIQAAVLAGDAETGITIMQMDAGLDTGPMLLRGTMPIGPAT
                     TSADLHDGLAELGARLVLRALRENPAPEVQSAEGATYAPKLTREDGRIDWVQDAARIE
                     RQVRAFDPWPGTFTMLRGAMLKVLRAEPADGAGTPGTVLDRHLAVACGAGALRLTHVQ
                     LAGRPAMAADAFLRGHPVAPGIVLGD"
     gene            complement(2840..3376)
                     /gene="def"
                     /locus_tag="VKQ27_14265"
     CDS             complement(2840..3376)
                     /gene="def"
                     /locus_tag="VKQ27_14265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLY90143.1"
                     /translation="MNETVETLPILLVPDPVLKARAKPVTPEEMERVRALIPRMFVTM
                     YRAPGIGLAAPQVGVALRCAVVDLMADNVSKPIALINPEVIARSDELVTREEGCLSLP
                     GQYAEVTRPARVMVRHLDLDGVRRQIEAEGLLSACLQHEIDHLDGVLFVDHLSALKRN
                     MILRRLAKELRQKRTEAM"
     gene            3511..3972
                     /locus_tag="VKQ27_14270"
     CDS             3511..3972
                     /locus_tag="VKQ27_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HLY90144.1"
                     /translation="MTTRMFTSPLFLGFDHLEQMLERASKSSSDGYPPYNIEQTGPAG
                     LRITLAVAGFTMDDLQITQEDNQLVIRGRQNDDSQGRVFLHRGIAARQFQRAFVLAEG
                     IEVKGAWLDNGLLHIDLIRPQPESRVKTIQIDRAASTGSGRVKMVEGESES"
     gene            4063..4320
                     /locus_tag="VKQ27_14275"
     CDS             4063..4320
                     /locus_tag="VKQ27_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HLY90145.1"
                     /translation="MDTNSIQSAEGQAVVAATVDIRHISTEQLAQLGLQQIAYVKPVV
                     VNGTAAFAIHAADGTPMAIAGDREVAIAAIQQHEMVATQVH"
     gene            complement(4400..4978)
                     /gene="raiA"
                     /locus_tag="VKQ27_14280"
     CDS             complement(4400..4978)
                     /gene="raiA"
                     /locus_tag="VKQ27_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLY90146.1"
                     /translation="MQITVSGKQVDLSDALRTRVSDHLDVIASKYFDHALEAQVTFSR
                     ARSFFTCDINVHAGRGLRLRGEGEAADANTAFDDAAEHIAKRLRRYRRRVNEHARDCA
                     NRERPEAARQYILRQEDAATEGDSGNKTTTHATVIAEQVTEISVLSVSDAVMRMDLAD
                     QPVLMFRNSTTGELNVVYRRSDGNIGWIDPSE"
     gene            complement(5039..6538)
                     /gene="rpoN"
                     /locus_tag="VKQ27_14285"
     CDS             complement(5039..6538)
                     /gene="rpoN"
                     /locus_tag="VKQ27_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLY90147.1"
                     /translation="MALGPRLDLRQSQTLVMTPQLRQAIKLLQYSNLEVATFVEEELE
                     RNPLLERDDTTELPNTDRAAPDQTPERVSETVDAARLAEAETLPSEAAAPLDADMSDT
                     FDAGGVADGASIQTAISGRGGASDFENDDRGVEDIADEVRPLRDYLGEQLRLSFADPV
                     DRMIGAHLIALLCPAGRLTAEPAAIAAAMVLPLERIESVRMRMMRFDPVGLFARNLKE
                     CLAAQLAEKNRLDPAMQALLDNLDLLARRELRRLSSICGVDADDLADMIAEIRSLDPK
                     PAASYDSTPTQLVVPDVLMRPGPGDTWLIELNPDTMPRLLVNERFYARVAPRARKADR
                     LFLAEQLASANWLVRSLQQRAQTILKVAAEIIRQQDGFMRHGVAHLRPLILRDIAETV
                     EMHESTVSRVTANKYIATPRGTIELKYFFTTAIPGTDGESHSAEAVRYRIRALIDAET
                     AESVLSDDALVAALRDEGVDIARRTVAKYREALRIPNSVQRRREKAVLV"
     gene            complement(6619..7389)
                     /gene="lptB"
                     /locus_tag="VKQ27_14290"
     CDS             complement(6619..7389)
                     /gene="lptB"
                     /locus_tag="VKQ27_14290"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HLY90148.1"
                     /translation="MRSEPRLPAELRIVSGGGGLAASGVGKTYKKRPVVRNVSINLRR
                     GEAVGLLGPNGAGKTTTFYMMIGLVKPDTGSISLDGADITSLPMYRRARLGIGYLPQE
                     ASVFRGLNVEQNVMAVLEAVEPDPDKRDLMLDELLAEFGIGHLRRTPALALSGGERRR
                     VEIARALATQPAYILLDEPLAGIDPIAVNEIRDLVSHLKDRGIGVLITDHNVRETLGI
                     IDRAYILHDGQVLMEGRPDEVVAHEDVRRVYLGERFSL"
     gene            complement(7395..8378)
                     /locus_tag="VKQ27_14295"
     CDS             complement(7395..8378)
                     /locus_tag="VKQ27_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HLY90149.1"
                     /translation="MSRIAWLLPLTLLVPVLGGAPARAQQLDLSHGGPIAITATDGIE
                     WRQNQREVIARGNARAVRQNVTVSADQLIAYYRPKNGTAPATAQPAANPVGADADAGG
                     NEIYRLEAVGNVHIFTPTDQAQGDRAIYDIDQAVLVMTGHDLKLTTPNDVLTARDTLE
                     YWSQKHMAVARGDAVVTTNDGKRLSADTLVAYTTDAQQPPAAATQVAAKTPAPTDNND
                     DPLAASGKLQKVELFGNVIVRTPTDTAIGDRAVYVPDTGIARLAGKVRLTRGQNQLNG
                     TEAEVNMKTGISRLLSAAKGGVHGLVVPNDATNQQLTAPGDPTGSPAGKSR"
     gene            complement(8375..9055)
                     /gene="lptC"
                     /locus_tag="VKQ27_14300"
     CDS             complement(8375..9055)
                     /gene="lptC"
                     /locus_tag="VKQ27_14300"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HLY90150.1"
                     /translation="MSVTPLPARAMPSRRHGHQLIAAAAARVRQVPSPSGIARRRALI
                     TLTKWLLPICALLLLASIALWPELGRVRDTVRMTIEHSGASGATLTDARYHGIDEHGR
                     PYTVTAETAQQDGPDRINLTIPKADITLSNGTWLMVQSKRGVYLQHTSQLDLSRDVTL
                     YRDDGTTLTTASVSIDLREGAGASDQPTHAEGPFGTLDAQGFIVTDKGTAIQFTGPAR
                     LVLNGTQQ"
     gene            complement(9052..10050)
                     /locus_tag="VKQ27_14305"
     CDS             complement(9052..10050)
                     /locus_tag="VKQ27_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HLY90151.1"
                     /translation="MAATRADATRSALTDDVAVARSVLRTEAAGLQVLAGSLGDSFAR
                     AVDLLAAATGRVVVSGMGKSGHVGRKIAATLASTGTPALFVHPAEASHGDLGMIVPGD
                     AILALSNSGETAELADLVAHSRRFALPLVAITARAGSALARAADLALTLPAAVEACPM
                     GLAPTTSTTMQMALGDALAVALLTRRGFTEADFRQFHPGGRLGTRLRRVGDLMHHGDA
                     VPLAAPSTAMDRALLLMTEKRFGCLGVIDGAGRLVGIVTDGDLRRAMGPDLLSRRVGD
                     VMTRSPRTIRADALAEEALHLMNARERPITSLFVVDESDRPLGILHVHDLLRAGIA"
     gene            complement(10178..10813)
                     /locus_tag="VKQ27_14310"
     CDS             complement(10178..10813)
                     /locus_tag="VKQ27_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HLY90152.1"
                     /translation="MPGGTIHLHRHDLPEGLELGPMVAVDTETMGLNPHRDRLCLVQL
                     SAGDGHAHLVQIIPPSLGGHGYHCPNLSRLMADPATVKLMHFARFDVAMLQHSLGITV
                     APVRCTKIAARLTRTFTDRHGLKDLCKELLGIDLSKQQQSSDWGAVELTPEQMAYAAS
                     DVLHLHALWAKLESLLIRERRRELAEACFDFLPARARLDLLGYEQPDIFSH"
     gene            complement(10855..10941)
                     /locus_tag="VKQ27_14315"
     tRNA            complement(10855..10941)
                     /locus_tag="VKQ27_14315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10905..10907),aa:Leu,seq:cag)
     gene            complement(11114..13258)
                     /locus_tag="VKQ27_14320"
     CDS             complement(11114..13258)
                     /locus_tag="VKQ27_14320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY90153.1"
                     /translation="MIVASVIVPLLVFVAGSWLAWRSTVQNAEADVRGSLAVSSEQVT
                     RVLETHVLLGDRINDILGNVPDAQVVAHERELHDRLAAMMAGYSQVTAVIVLDADGHP
                     LVATSRYPVDRAANFSDRDYFRTLRDTGIAMEIGGLVHGRLTQRDLFSVAIRRGAAGQ
                     TFAGAILIGVSPSYFADFDRTLFAGDLDYTAGLLRSDGTVLASYPDTMAANENPMRDQ
                     MMLDAIARAPMEGLVDGSSPAVGTPRLIAYRRLANFPLIVTVGRRWSSVVLEWRNIMA
                     THLIFGVPATLCLLLLSILATRRWQRQHETLAQLRSEVQRRELAEEALRQSQKMEAIG
                     RLTGGVAHDFNNHLTVISSNIELLQRRMPHGSEALARLADAAMSGVQRAATLTHRLLA
                     FARQQPLDPEPLDLGRLVTGMSDLLRRTLGEDIAIETVLAGGLWQSRVDANQLENVLL
                     NLAVNARDAMPDGGKLTIETANAHLDDTYAALHTEVAAGQYVMLAVTDTGIGMAPEVI
                     EKAFEPFFTTKPLGQGTGLGLSMVYGFIKQSGGHVAIYSEPGQGSTVKVYLPRFVRPD
                     MKPAAGDDAGRSRPRGSGETILVVEDDEDVRRASVEALHEMGYEVLEAGDAMDGVRLI
                     VDRGDIDLLFTDVGLPGGVNGRALADAARSAQPGLRVLFTTGYTRNAILHNGVLDPDV
                     HFIAKPFNLTALAAKVREVLDTPADTPEGHVL"
     gene            13430..14386
                     /gene="rbsK"
                     /locus_tag="VKQ27_14325"
     CDS             13430..14386
                     /gene="rbsK"
                     /locus_tag="VKQ27_14325"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLY90154.1"
                     /translation="MRNLARPVLVIGSLNMDLVVRCERLPSEGETVFGSDFFTAFGGK
                     GANQAVAAARLGAQVAMAGCVGNDQFGTDLIAGLTAAGVQTGSVLVADRPTGTALITV
                     NSVGANTIVVVSGANIACGKELIDRALADTATPGVLLLQHEIPAAANEYAIRTAKAAG
                     WFVVLNPAPARAVPPDLLPLIDIIAPNETEAAMLTGRSIAGRADALAAVDVLLARGAG
                     AVLITLGRDGAVYRDATRALHCPALPAQAVDTTAAGDAYLGALGAALAEGSTVEQGLG
                     FAAAAAGLAVTRLGAQPSLGTRAEVTAFIARYGQPQPVSVPG"
     gene            complement(14370..15440)
                     /locus_tag="VKQ27_14330"
     CDS             complement(14370..15440)
                     /locus_tag="VKQ27_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLY90155.1"
                     /translation="MVPVSTRHASEERSLEGALSPTQAGSAPTERPDALSALVSLLEP
                     DLEACNRTIVARMDSPVKLIPQLAAHIVAAGGKRLRPLLTLASARLCDYQGDRHIALA
                     ACVEFIHTATLLHDDVVDESLLRRGLASANAVFGNQASVLVGDFLFARAFQLMVKDGS
                     LTVLAILSRAAATIAEGEVLQLVTQNDLSTSEARYLEVIRGKTAALFAAACEVGGVVA
                     SRPDHEVAALAEFGMNLGIAFQLVDDALDYAADQAVLGKTVGDDFREGKITLPVLAAY
                     HAGDGEERAFWERTIEATEQTEADLDHALHLMARRDAIGVTLERACGFAAAAKAALAI
                     FPDNALRRALVDVADYTISRAR"
     gene            15471..15677
                     /locus_tag="VKQ27_14335"
     CDS             15471..15677
                     /locus_tag="VKQ27_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLY90156.1"
                     /translation="MRELVNSNDPVRLSFLTALLADAGIMAVLLDVHTSAIEGSIGAI
                     PRRLMVAAEDERRAREVLREAGEL"
     gene            complement(15877..>16504)
                     /locus_tag="VKQ27_14340"
     CDS             complement(15877..>16504)
                     /locus_tag="VKQ27_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HLY90157.1"
                     /translation="GRVGQQLDGLHSIAELDIGRFIITPNQNLVLADIPVDRKAEVEA
                     WLSAHHLDSMVSGLRRNALACVALPTCGLALAESERYLPDLITRLENELEQSGLAQDD
                     ITIRMTGCPNGCARPYLAEIGLVGRTPGIYNLYLGGAFNGTRMNKLYRRDVNGDAIVA
                     ALAPLFRGYATQREPAESFGDFVIRAGHVRPTTAGNRFHADLAPELQG"
BASE COUNT         2885 a         5421 c         5245 g         2953 t
ORIGIN      
        1 ccaatgtcgt ggccgaccag aaggatgtcg ccggatgcca ggcggcgggc gatgacgcgc
       61 gcggtctgca gctcacggcc gcggttgtcc acgcgcacca gctcaactgc tcggggcgac
      121 gctcccagtg gcaggcgcgg cggcagagat gcaacgttgc cggcgatcgg tgcgccgtcc
      181 ggcgcgaaca gttccgccag cttcactcgg cgaggatcgt cttccaggtg ctcggtcacg
      241 aaatgcagcg cctgtgtctg cggcaggctc ggatcgaagt gtgagttggc gagaacgatc
      301 gtgtcgactt tgccggtcac gtacatcgct gtttgccagt agacgaagcc gaacaagagt
      361 acggtgcaga caccgaatgc cccagcaacg gcgagcgccc agcggacggt cgtagaatgg
      421 aagacgtcag ccgggcgcac ggagcacgaa tcccgcgcca cgcacgctca ggatcatcgg
      481 cggctcatca ggtgcatcga ctttgcggcg cagtcggccg atatgcacgt ccacaagatt
      541 tgtttgcggc acgaaccgat agttccacac attttccagc agcatggcgc gggtcaggac
      601 ctgtccttcg cggcgcatca tgtactcgag caactggaat tcgcgtggca acagatccag
      661 ttcgcggtgg ccgcggtgcg cggcgcgcgc gagaagatcg agttccagcg gaccgatacg
      721 cagcacagtt tcacgtggat ccgccgggcg gcgcagcaat gcctcaagcc gcgcggccag
      781 ctcggataag gcaaacggct tggcgagata atcgtcgccg cctgcgcgca atccgcgcac
      841 ccgatcgttc accgcattga gagcgctgag cacaagtact ggtgtcttga tgccttcgtg
      901 ccgcagggtc tcaacaacgg tcagcccgtc gagacctggc agcatacgat ccaccaccag
      961 cacgtcccaa ccgcccgcgc gggcccgttg caggccctcg gtgccatccg ccgcacggtc
     1021 cagcgcgtag ccgcgatcgt cgagtgccgt tacgatttcc tcggcggtac catcatcgtc
     1081 ttcaataacg agcactttgg ccatggcgac cttgcgcatg taggttgttc atggcgcgag
     1141 gccaaccttc tcgaataggt ccgtaatgta tcgaactgca atcaggcaca gcccgtccgg
     1201 cggtgcggtt ggtcctgccg ccgagcggtc tcgtgccgac aacgcagcag cgacacgctc
     1261 gatcggccag ctcccatcgc ccaccagctt caacgtgccc accatgttgc gcacctggtg
     1321 gtgcaggaaa ctgcgcgctt ccgcgacgat ctcgatgccc tcgccgtcgc gtctcacatc
     1381 caggcgatcc agggtgcgca acggactatt ggcttggcaa gcggccgcgc ggaacgaagt
     1441 aaagtcatgt cggccaagca atgtctgcgc ggccgcgtgc atggcggcgg cgtcaagcgg
     1501 ggcgggcaca tgccagacgc ggccagcgag cagggcaggg cgggcgcggc ggttcaggat
     1561 gcgatagcga tagacgcggc cgaccgccga gaagcgagcg ttccactcgt ctggcgccgg
     1621 cgcaacctga agcacggcca ccggatgcgg cttcatgtgg taattcagcg cgtctcggac
     1681 tttgccggcg taaatggccg cgggtagctc caattgcacc acctgttccg cggcatgcac
     1741 gccggcatcg gtgcggcccg ccacggtggt ggagacgggc ttgccgcgat tgagcttttc
     1801 ggcagcgttt tccagcacct cttgtaccga cagcccggtc gcctgccgct gccatccaac
     1861 gaacgggccg ccgtcgtatt ccaacaggag ggcccagcgt gcgcgcgccg tagggtcagc
     1921 gaccacctag tcccccagca cgatcccagg tgccaccggg tgcccgcgga gaaacgcgtc
     1981 cgctgccatc gcaggtcgac cggccaactg tacatgcgtc agccgcaggg ccccggcgcc
     2041 acaggcaacg gccaggtgtc tgtccagcac cgttccgggc gtgcccgccc catccgctgg
     2101 ttcggcccgc aataccttca gcatcgcccc acgcaacatc gtgaatgtcc caggccacgg
     2161 gtcgaacgcc cgtacctgcc gctcgatccg cgccgcgtcc tgcacccagt cgatccgccc
     2221 gtcctcacgc gtcagctttg gcgcgtaggt tgcgccctcc gctgactgca cctctggtgc
     2281 tggattctcc cgcaatgcgc gcagcacgag ccgcgcgccc agctctgcca gcccgtcatg
     2341 cagatcggcc gaggtcgtcg cgggcccgat cggcatcgtc ccgcgcagca gcattgggcc
     2401 ggtatcgagg ccggcatcca tctgcatgat cgtaatcccg gtctcggcat cgccggccaa
     2461 tacggctgcc tggattggag ccgcgcccct ccatcgaggc aacaggctgg catggatgtt
     2521 aaggcaccct cgccgcggtg ccgccagcat cgccggaggc aggatcaggc cgtaggcggc
     2581 cacaacggcc gcatccaggt cgagggcggc gaacgcctcc tgcgttgccg ggtcggtgcg
     2641 caggcggcgc ggcgtccgca ctggcaaccc cagcgctccg gccgccgcct gtaccgggca
     2701 cggccgcagc gcatatccgc gtccggctgg cttgggcggc tggcaatata cggcggcaat
     2761 ctcgtatccc gccccatgca gcgtgcgcag tgccgggacg gagaaatccg ggctgcccat
     2821 gaacgcgagg cgcatcggcc tacatcgcct cggtgcgctt ctggcggagt tccttggcca
     2881 gccggcgcag gatcatattg cgtttaaggg cggacaggtg atcgacgaac agcaccccat
     2941 ccagatggtc gatctcatgc tgcaggcagg ccgagagcag cccctcggcc tcgatctgcc
     3001 gccgcacgcc gtccaggtcc aggtgccgta ccatcacgcg cgccggccgg gtcacctcgg
     3061 catattggcc aggcagcgac aggcagcctt cctcacgcgt caccagttcg tcgctgcgcg
     3121 cgatcacctc cgggttgatc agcgcgatcg gcttcgacac gttgtccgcc atcaggtcga
     3181 ccaccgcaca ccgcagcgcc acacccacct ggggtgcggc cagcccaatg ccgggcgccc
     3241 tgtacatcgt gacgaacatc cgcgggatca gcgcccgtac ccgctccatc tcctctggtg
     3301 tgaccggctt ggcccgcgcc ttcaggaccg ggtcgggcac cagcaggatg ggcagcgtct
     3361 ccactgtctc gttcatgtcg cgcatgtagc ggtggtgcca cacgtgatca atgtctcacc
     3421 ccttgtaacc gacaccgacc ctgccaagat ttccacctgt cgggatgccg ttgcgggttc
     3481 cggcactgct tcgctctcag gagggggccc atgacgacga ggatgttcac ctcaccgttg
     3541 ttcctaggtt tcgaccatct cgagcagatg ctcgagcggg catcgaaaag ctcgtcggac
     3601 ggctacccgc cttacaacat cgagcagact ggccctgcag ggttgcggat cacgctcgct
     3661 gttgcgggtt ttaccatgga cgatctgcaa attacgcagg aagacaacca gttggtaatc
     3721 cggggcaggc agaatgatga cagtcaagga cgcgtatttc tgcatcgcgg gatcgcggct
     3781 cggcagtttc agcgggcctt cgtgctcgcc gaagggatcg aggtgaaggg tgcgtggctc
     3841 gataacgggc tgctgcacat cgacctgatc cggccccagc cggaatcgcg cgtgaagacc
     3901 atccagatcg atcgcgctgc atcgacgggc agcggacggg tcaaaatggt ggaaggcgag
     3961 tcggaaagct gagttgctga cggcgccgca gaaggcgggc cgaaccggaa aaaggtaccg
     4021 gggctgcaat gcgcaggtcg gtgcccggaa ggagcaaggg caatggatac gaacagcatt
     4081 caatcagccg agggccaggc ggtcgtggcg gcgacagtgg atatacgaca catttcaacg
     4141 gagcaattgg cacagcttgg cctgcagcag atagcctatg tcaagccagt ggtggtgaac
     4201 gggaccgcgg ccttcgcgat tcatgccgcc gacggcacgc cgatggcgat cgcgggcgac
     4261 cgcgaggttg cgatcgcggc gatccagcag cacgagatgg tagcgacaca ggttcactga
     4321 ggagcggctt attttcctca ccctcgcggg cgcagaggcc ttcccccggg ctcgagccgg
     4381 ggggaagggc gatgagggcc tactcgctgg ggtcgatcca gccgatattc ccatcactcc
     4441 gacggtatac gacattgagc tcacccgtgg tgctgttgcg gaacatcagc accggctgat
     4501 cggcgagatc catgcgcatg accgcgtcgc ttacggacag gacgctgatt tccgtaacct
     4561 gttcagcgat caccgtggca tgcgtcgtgg tcttgtttcc gctatccccc tcagtcgccg
     4621 catcctcctg gcgaaggatg tattgccgcg ctgcttcagg ccgctcgcgg ttcgcgcaat
     4681 cgcgcgcatg ttcgttcacg cgccgccggt agcggcgtag ccgcttggcg atgtgctccg
     4741 ccgcatcgtc gaatgccgtg tttgcatcgg cagcttcgcc ctccccccgc aaccgcagcc
     4801 cacgtcccgc atgcacattg atgtcgcatg tgaagaagct gcgcgcgcgg ctgaacgtga
     4861 cctgcgcttc cagcgcatgg tcgaagtact tactggcaat tacatcgaga tggtccgata
     4921 cgcgcgtgcg aagcgcgtcc gacagatcga cctgcttacc ggaaaccgta atttgcatcg
     4981 tgcctctccc cgctgtggat cgcgggagaa gcccttgtcc ccgggcttgc cccggaggct
     5041 acacaagcac ggccttctcc cgccggcgct gcaccgagtt ggggatacgc agcgcctctc
     5101 gatatttggc cacggttcgc cgagcgatgt ccacgccctc gtcccgcagt gccgcaacaa
     5161 gcgcatcatc agacagaaca ctttccgctg tctccgcgtc gatcaacgca cgaatgcgat
     5221 agcgtacggc ttccgcgctg tggctttcgc catccgttcc tgggatcgcc gtggtgaaaa
     5281 aatacttaag ctctatcgtg ccccgtggcg tggcgatata tttgttggcc gtcacccggc
     5341 tgacggtgct ttcgtgcatc tccaccgttt cggcgatgtc gcgaaggatc aatggccgca
     5401 gatgcgccac gccatgccgc atgaatccat cctgctggcg gatgatctct gcggcgacct
     5461 tcaggatcgt ctgcgcgcgc tgttgaagcg aacgcaccag ccaattggcg ctggcaagct
     5521 gttcggcaag gaacagccga tccgccttgc gtgcacgtgg tgcgacacgc gcatagaagc
     5581 gttcgttcac gagcaggcgc ggcatggtgt cgggattgag ctcgataagc caggtatcgc
     5641 cagggccagg ccgcatcaga acatctggca ccaccagctg tgtcggcgtc gaatcgtagc
     5701 ttgccgccgg cttcgggtcg agtgatcgga tctccgcgat catgtcggcc agatcgtcgg
     5761 cgtcaacgcc gcatatcgat gacagccgcc gcagttcacg ccgcgccaga agatcaagat
     5821 tgtcgagcag cgcctgcatt gcaggatcca accggttctt ctccgcgagc tgtgctgcca
     5881 ggcactcctt caagttgcgc gcaaacaatc ccaccggatc gaaccgcatc atgcgcatgc
     5941 gcaccgactc gatgcgctcc agcggcagta ccattgccgc cgcgatcgct gcgggttcgg
     6001 cggtcagacg tcctgccggg cacagtaatg caatcaggtg cgcaccgatc atccggtcca
     6061 ccggatcggc aaacgacagg cgcaactgct cgccgaggta gtcgcgcaac ggacgcactt
     6121 catccgcgat atcctcgacg ccgcgatcgt cgttctcgaa atcactcgcg ccgccgcgtc
     6181 cgctgattgc cgtctgtatc gacgcgccat ccgctacgcc accggcatca aacgtgtcgg
     6241 acatgtcggc atcgagtggt gctgctgcct cgctcggcag ggtttcggcc tcggcaaggc
     6301 gtgccgcatc gacagtctcg ctcacgcgct ctggtgtctg gtcgggcgcg gcccggtcgg
     6361 tgttcggcaa ctctgtggtg tcatcccgct ccagcagcgg attccgctcg agctcttcct
     6421 caacgaaggt cgcgacttcg aggttggaat attgcagcag cttgatcgcc tggcgcagtt
     6481 gcggcgtcat gacgagcgtc tggctctggc gaagatcgag gcgcggacct aacgccatgg
     6541 cccccgcgtc ctggtcagat catccatgca gcacgacatg ctacgctgtc gtcgcaaaca
     6601 aaccgttcac atatgcggtc aaaggctgaa cctctcgccg agatagacgc ggcgcacatc
     6661 ttcgtgtgct accacctcgt ctggccgtcc ctccatcagc acctgaccgt cgtgcaggat
     6721 ataagcgcgg tcgatgatcc cgagcgtttc gcgaacattg tggtcggtga tcagcacgcc
     6781 gatgccgcga tccttcagat gcgacaccag gtcgcggatt tcgttaacgg cgatcggatc
     6841 gatgcctgcc agcggttcgt ccagcaggat gtaggccggc tgcgttgcca gtgcgcgtgc
     6901 gatttccacg cgccgccgct caccccccga tagtgccagc gccggtgtac gccttagatg
     6961 gcctatgccg aactcagcga gcagttcgtc aagcatcagg tcgcgcttgt cgggatccgg
     7021 ctcgacggcc tccagcaccg ccatcacgtt ctgctcgaca ttgagcccgc gaaacacact
     7081 ggcttcctgt ggaagatagc caatgccaag ccgtgcgcgc cggtacatcg gcagactggt
     7141 gatgtcggcg ccgtcaaggc tgattgagcc ggtgtcgggc ttcacaaggc cgatcatcat
     7201 ataaaacgtc gtggtcttgc cggcgccgtt cggccctagc aggcccacgg cctcgccgcg
     7261 gcgcaggtta atcgagacgt tccgcacgac cggccgcttt ttgtaggtct tgccgacgcc
     7321 gcttgccgcc aacccacctc cgccagatac aatccgcaac tcggcgggca aacgcggttc
     7381 cgagcgcatg tcgttcatct tgattttccc gccggagaac ccgtcggatc gcctggtgca
     7441 gtcagctgtt gattggtcgc atcgttcggc accacgagac cgtgcacgcc acctttcgca
     7501 gccgacagca ggcgcgagat gccagtcttc atgttgactt ccgcctctgt cccgttgagt
     7561 tgattctgcc cccgcgtgag gcggaccttg ccagcgaggc gagcgatgcc ggtatccggg
     7621 acatacaccg cacgatcgcc gatggcggtg tccgtcggag tgcggacgat gacgttgccg
     7681 aacaactcta ccttctgaag cttgcccgat gcagcgagtg gatcgtcgtt gttgtccgtt
     7741 ggcgcaggcg tctttgccgc cacctgggtt gccgctgccg gtggctgctg agcatcagtt
     7801 gtataggcga ccagcgtatc cgcagacagt cgcttgccgt cgttcgttgt aacgaccgca
     7861 tcgccacgcg ccaccgccat gtgcttttgc gaccagtatt ccaatgtgtc gcgcgccgtc
     7921 agcacatcgt tcggcgtagt cagtttcaga tcatggccgg tcatcaccag cacggcttga
     7981 tcgatgtcgt agattgcgcg gtcgccctgc gcctgatcag tcggcgtgaa gatgtggaca
     8041 ttgcccaccg cctcgagccg ataaatctca ttgccaccgg cgtccgcatc agcacccacc
     8101 gggttggccg cgggctgagc cgtcgcggga gcagtaccgt tcttcggacg ataataggcg
     8161 atcaactgat ccgctgagac ggtcacgttc tgcctgacgg cgcgggcgtt gccgcgggcg
     8221 atgacctcgc gctggttttg gcgccattcg attccgtccg ttgcggtaat cgcgatgggg
     8281 ccaccatgcg acaggtcgag ttgctgtgcg cgggcgggcg ccccgccgag caccggcacc
     8341 agcagcgtta gaggcaggag ccaggcgatc ctgctcattg ctgtgtgccg ttcagcacca
     8401 gtcttgccgg cccggtgaac tggattgcgg tgcctttgtc ggtgacgatg aagccctggg
     8461 catcgagcgt gccaaacgga ccttcggcat gcgtcggctg gtcgctcgcc cccgctccct
     8521 ccctgaggtc gatcgacacg ctcgcagtcg tcagggtcgt tccatcatcc cgataaagcg
     8581 ttacgtcacg ggacagatcg agctggcttg tgtgttgaag atacaccccc cgcttgcttt
     8641 gcaccattag ccaggtgccg tttgagagcg tgatgtcggc cttgggtatc gtcaggttga
     8701 tgcgatcggg tccgtcctgt tgcgccgtct ctgccgtgac ggtgtaaggc cgcccgtgct
     8761 cgtcgatgcc gtgatagcgg gcgtccgtga gcgtggcgcc gctcgcgccg gaatgctcga
     8821 ttgtcatgcg gaccgtatcc cgcacacggc ccagttccgg ccatagggca atgcttgcca
     8881 gcagcaaaag ggcacagatc ggcaggagcc atttggtcag cgtgatcaac gcgcgccgtc
     8941 gcgctatgcc gctcgggctc ggaacctggc ggaccctggc ggccgcggcg gcgataagct
     9001 gatggccgtg gcggcggctg ggcattgctc gggcggggag gggcgtgacg ctcatgcgat
     9061 cccggcccgc aacaggtcgt ggacgtgcaa aatgccaagc ggtcggtcgg actcgtccac
     9121 gacgaacagc gaggtgatcg ggcgctctct ggcattcatc agatgcaacg cctcctccgc
     9181 cagggcatcg gcgcgtattg tccgtggcga gcgggtcatt acgtcgccta cccggcgcga
     9241 caaaagatct ggccccatgg cgcggcgcag gtccccgtcg gtcacaatgc cgaccaggcg
     9301 tccggctcca tcgatcaccc caaggcagcc gaagcgcttt tcggtcatca gcagcagtgc
     9361 ccgatccatg gcagtgctcg gcgccgccag gggcaccgca tccccatggt gcatgaggtc
     9421 gccgacgcgg cgcagacgcg tgccgagccg gccaccagga tgaaattgcc tgaagtccgc
     9481 ctcggtaaac ccccggcgcg tcagcaaagc aactgccagg gcgtcgccca gcgccatctg
     9541 catggtggta gacgttgtgg gtgccagccc catcggacat gcctcgacag ccgccggtag
     9601 cgtcagggcc agatccgcgg ctcgcgccaa cgccgagccg gcgcgggcgg taatggccac
     9661 cagcggcagc gcgaagcggc gcgaatgcgc gaccaggtcc gcgagctccg ctgtttcgcc
     9721 cgagttggac agcgccagta tggcatcgcc tggcacgatc atgcccaggt cgccgtggga
     9781 cgcctcggcc ggatgcacga acaacgcagg tgtgccggtc gaggccagcg tggcggcgat
     9841 cttccgccca acgtggcccg atttgcccat cccggaaaca accacccgcc cagtggcagc
     9901 cgccagcaga tccacggcac gcgcgaagct gtcgcccaag ctgccggcga gcacctgcaa
     9961 cccggcggcc tcggtgcgca acacgctacg ggcaacggcc acatcgtcgg tcagcgccga
    10021 tcgcgtggca tcggcccggg tggcggccat tgcgcctgtc atcgtatggc gacagcttgg
    10081 ttcatgcttc gtctgttttg actgtcgtta aggagccggt caacctcaat gcgcggctcc
    10141 gacaagaacc gtgccaattc ttaaggttgc cgcagcatca atgggaaaag atgtcgggct
    10201 gctcgtaacc aagcaggtcg agtcgggctc gggccggcag aaaatcgaaa catgcctcag
    10261 ccaattcgcg ccggcgctcg cggatcagca gcgattcgag cttcgcccag agcgcgtgga
    10321 ggtgcagcac atccgacgca gcatatgcca tctgctccgg tgtcaattcc accgcgcccc
    10381 agtccgacga ctgttgctgc ttcgacaggt cgatgcccag cagctctttg cacaggtcct
    10441 ttagcccatg cctgtcggtg aaggtacgtg tcagccgcgc ggcaatcttg gtgcagcgca
    10501 ccggtgcgac agtgatcccc agcgaatgct ggagcatcgc cacatcgaag cgcgcaaagt
    10561 gcatcagctt caccgtggcc ggatcagcca tcaggcggct cagattcggg cagtgatagc
    10621 catggccgcc gagcgaaggc gggatgattt gcacgaggtg cgcatgcccg tcaccggccg
    10681 agagctgcac caggcaaagg cggtcgcggt gcggattcag gcccatcgtc tcggtatcga
    10741 ccgccaccat cgggccgagc tcgaggcctt cgggcaggtc gtgccgatgc aggtggatgg
    10801 tgccgccggg catgaattgc gttgtgctca cggattcgga gtttatccgc cgcttggtgc
    10861 ccaggagaag actcgaactt ccacgacctt gcggccacag gtacctgaaa cctgcgcgtc
    10921 taccaattcc gccacctggg cattgatggt gcagcgatgg ggcgcgcgat gtagcgtccg
    10981 ggcgctggca cgtcaacggc agcgcggtgt gttgcctcgg gcagcggtcc tggagaccac
    11041 cggaggcacg cgggcgccgg ccttccggat aagcgggcag cggggaaaac gccgcggcga
    11101 tccggcagcg ggactataga acgtgcccct ccggtgtgtc ggctggcgta tccagcacct
    11161 cgcgcacctt ggcggcgagt gcggtcaggt tgaatggctt ggcgatgaaa tgcacgtccg
    11221 gatcgaggac gccattatgc agtattgcgt tccgcgtgta gccggtggtg aacagcacgc
    11281 gcagtcctgg ctgcgctgac cgtgcggcat ctgccagggc acgtccgttg acaccgcccg
    11341 gcaagcccac atcggtgaag agcagatcga tgtcgccgcg atcgacgatc agacggaccc
    11401 cgtccatcgc atcgccagcc tccagcacct cgtaacccat ctcgtgcaac gcctcgacgg
    11461 aggcgcggcg aacatcctcg tcatcctcga cgacaaggat tgtctcgccg ctaccgcgcg
    11521 gtcgtgaccg acctgcatcg tcgccggccg cgggtttcat atcgggacgt acgaagcgtg
    11581 gcagatacac cttcaccgtg ctgccttgcc ctggctcgct gtaaatagcg acgtggccgc
    11641 cagactgctt gatgaagccg tacaccatcg agaggccaag tccggtaccc tggcccagtg
    11701 gcttggtggt aaagaacggc tcgaacgcct tctcgatcac ctcgggcgcc atgcctatcc
    11761 cggtatccgt cactgccagc atcacgtact gaccggcggc cacctcggtg tgcaaggctg
    11821 catacgtgtc atcgagatgc gcattggcgg tttcgatcgt caacttgccg ccgtccggca
    11881 tggcatcgcg cgcattcacc gcgagattca gcaaaacgtt ctcaagctga ttcgcatcca
    11941 cgcgcgattg ccacagaccg ccagccagaa cggtctcgat cgcaatgtcc tcgcccagtg
    12001 tccgacgcag caggtccgac atgcctgtca ccaggcgccc gaggtccaac ggctccgggt
    12061 ccaacggctg ctgccgggca aatgccagca gccgatgtgt cagcgtagcc gcccgttgca
    12121 cgccggacat ggcagcatcg gccagtcttg ccagcgcctc gctgccgtgc ggcatgcgac
    12181 gctggagcag ctctatattg ctgctgatca ccgtcagatg gttgttgaag tcgtgcgcga
    12241 cgccgcctgt aagccggccg atcgcctcca tcttctgtga ttggcgcaat gcctcctctg
    12301 ccaactcgcg gcgctgcacc tcgcttctga gctgtgccag cgtctcgtgc tgtcgctgcc
    12361 atcggcgtgt cgcgagtatg cttagcagca gcaggcacag ggtcgccgga accccgaaga
    12421 tcaggtgcgt cgccatgatg ttccgccact ccaggaccac cgacgaccag cgccggccca
    12481 ccgtgacaat gagcggaaaa tttgccagcc tgcggtaggc gatcagccgg ggggtgccaa
    12541 cggctgggga cgagccgtca accaggccct ccatcggtgc gcgcgcaatg gcgtccagca
    12601 tcatttggtc gcgcatcgga ttttcgtttg ccgccatggt gtcgggatag ctggccagta
    12661 cggtgccgtc gctgcggagc aatcctgctg tgtaatcgag gtcgccggca aacagtgtcc
    12721 ggtcgaaatc ggcgaaatag ctcggcgaca caccgatcag aatggcccct gcgaacgttt
    12781 gcccagcggc gccgcggcgg atcgccacgc tgaacaagtc tcgctgcgtc aggcggccat
    12841 ggaccaggcc gccaatttcc atcgcaattc cagtatcgcg gagcgtgcgg aaataatcgc
    12901 ggtcgctgaa gttggccgcc cggtccaccg ggtagcggga tgtcgcgacc agtggatgtc
    12961 catcggcatc cagcacgatc accgcggtga cctgactgta gcccgccatc atcgccgcca
    13021 gcctgtcgtg caattcgcgc tcgtgcgcga cgacctgggc gtctggaacg ttgccaagta
    13081 tgtcgttgat ccgatcgccc aagagtacgt gggtttcgag tacccgtgtc acctgctcac
    13141 tggagaccgc cagggagcca cggacgtctg cctcggcatt ttgcacagtg ctgcgccaag
    13201 ccagccagct tcccgcaacg aacaccagca gcggcacgat aactgaggca actatcatgc
    13261 cgcgcagagc ccatggggtt ctggtctttc cgctcgcctc gctcataccc cgttgtcgcc
    13321 cccaagccgt cgcgaccatg ttgctcaatt gcgtgatgtc atagtacgga gtgtgtcgcc
    13381 agatgcacac gtttcgtttc tcacgcgcgc gtgatgccgc aagttccgca tgaggaacct
    13441 tgcgcgcccg gtcctggtca tcggcagcct caacatggac ctggtggtcc gctgcgagcg
    13501 cctgccgagc gaaggcgaaa cggtgttcgg atccgacttt ttcaccgctt tcggcgggaa
    13561 aggcgcgaac caggcggtcg ccgcggcgcg cctcggagcg caggtggcaa tggcaggttg
    13621 cgtcggaaac gatcaattcg gcactgatct gattgccggt ctgacagcgg cgggcgtaca
    13681 gaccggatcg gtcctggtgg ccgatcgtcc aacaggtaca gcgctgatca ccgtgaattc
    13741 tgtcggtgcc aatacaatcg tagtggtgag cggcgccaat atagcttgcg gcaaagagct
    13801 gatcgatcgg gcgctggccg acacggcaac cccaggtgtg ctgctgttgc agcacgagat
    13861 ccccgcggcg gcgaatgagt atgcaatccg tacggccaag gccgcggggt ggttcgtcgt
    13921 gctcaacccc gcgccggcgc gcgccgtgcc acccgatctg ttgccactga ttgatatcat
    13981 tgcaccgaac gaaacggagg ccgcgatgtt gaccggacgc tccattgcgg gccgggccga
    14041 tgcgctcgcc gccgtcgatg tgctgctggc gcggggagca ggcgcggtgc tgatcacgct
    14101 gggccgcgac ggcgcagtct atcgcgacgc cacgcgtgcg ctgcactgcc cggccctgcc
    14161 ggcgcaggct gtggatacca ctgccgctgg cgatgcctat ctgggggcgc ttggagccgc
    14221 gctggcggaa ggcagcactg tggaacaggg cctgggtttc gcagccgccg cggcgggcct
    14281 ggcggttacc cgattggggg cgcagccgtc actgggcacg cgcgccgagg taacagcatt
    14341 catcgcccgt tacggccagc cgcagcccgt cagcgtgccc ggctgatggt ataatctgcc
    14401 acatccacca gggcgcgccg cagggcatta tctggaaaaa tggccaacgc ggccttcgcc
    14461 gccgcggcaa agccgcatgc gcgctcaagg gtcacgccaa tggcgtcgcg acgcgccatc
    14521 aggtgcagcg cgtgatccag atccgcctcg gtttgctctg ttgcctcgat ggtgcgctcc
    14581 cagaacgcgc gctcctcccc gtcgcctgcg tggtaggcgg ccagaacggg caatgtgatc
    14641 ttcccctcac ggaaatcatc gccgacagtc ttgccgagca ctgcctggtc cgcggcgtaa
    14701 tccagcgcat cgtccaccaa ctggaacgca atacccaagt tcatgccgaa ctccgccaac
    14761 gccgccacct catggtccgg tcgagaggcc acaacaccgc ccacctcaca cgcggcggcg
    14821 aacagcgcgg cggtcttgcc gcggatcacc tccagatagc gcgcctcgct ggtggacagg
    14881 tcgttctgtg taacgagctg caatacctcg ccctcggcga tggtcgccgc ggcacgcgac
    14941 agtatggcga gcacggtcag cgagccgtcc ttcaccatca gctggaaggc gcgcgcgaac
    15001 agaaagtcgc ccacgagcac cgacgcctgg ttgccgaaca ccgcattcgc cgaggcgagg
    15061 ccacggcgca ggaggctctc atccaccacg tcgtcgtgca gcagggttgc ggtgtggatg
    15121 aactcgacgc aggcggcgag cgcgatgtgc cggtcgccct ggtaatcgca cagccgggca
    15181 gacgctagcg tcagcagtgg acgcagccgc ttgccgccgg ccgcgacgat gtgggcggcc
    15241 agttgcggga tcagcttaac cgggctgtcc atgcgggcaa cgatggtccg gttgcaggcc
    15301 tccagatctg gctccagcaa cgaaaccagg gcactcagcg catctgggcg ctctgtaggc
    15361 gccgaaccgg cctgcgtcgg gctgagcgcg ccctccaagc tcctctcctc gcttgcatgt
    15421 cgggtggaca caggcaccat atcggggccg cagaaacagg gtcaagcaag gtgcgtgaac
    15481 tggtgaactc gaatgatccg gtgcggctgt cgtttctgac cgccctgctc gcggatgctg
    15541 gcatcatggc ggtcctgctg gacgtccaca ccagcgccat cgagggcagt atcggcgcca
    15601 ttccccgccg ccttatggta gccgccgagg atgagcggcg cgcacgcgag gtgctccgcg
    15661 aggcagggga actgtgaggg gccctggcgc cggttcgggc gcgccgagcg tggaagtgcc
    15721 gcaatgggga tggcgatcgc gctaacaacc atcgggcgcc gtcgccactg catggggcga
    15781 cgaggcggcc agcctgatgc agcctcggac tggtcacccc gccgcctcgc ggcgctcgct
    15841 ggcagcctct atgtcggccg gcccgatgca gcaggatcag ccttgcagtt ctggagcgag
    15901 atcggcatgg aagcggttgc ctgccgtggt cgggcggacg tggccggcac ggatcacgaa
    15961 atccccaaaa ctctcggctg gctcgcgctg cgtcgcgtac cctctgaaca acggtgcgag
    16021 cgcggcaacg atggcgtcgc cgttgacatc gcggcgatac aatttgttca tgcgcgtgcc
    16081 gttgaatgcg ccgccgagat acaagttgta tatgccgggc gtgcggccga ccaagccgat
    16141 ctcggcgagg tagggtcggg cacagccatt tgggcaaccg gtcattcgga tcgtgatgtc
    16201 gtcctgtgcc aggcccgatt gttcgagttc attctccaga cgcgtgatga ggtcgggcag
    16261 atagcgttca ctttccgcca gcgcgaggcc acaggtcggt agcgcgacgc aggccagcgc
    16321 gttgcgccgc aagccgctga ccatgctgtc cagatggtgt gcgctcagcc acgcctcgac
    16381 ctcggccttg cgatcgaccg ggatgtcggc aagcacgaga ttctggttcg gcgtgatgat
    16441 gaagcgcccg atatcgagct ctgcgatgga atgcagcccg tcaagctgct ggcccacacg
    16501 accg
//
LOCUS       DATGEM010000450         4927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_753559, whole genome shotgun sequence.
ACCESSION   DATGEM010000450 DATGEM010000000
VERSION     DATGEM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753559"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="VKQ27_14345"
     CDS             complement(<1..2397)
                     /locus_tag="VKQ27_14345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY90158.1"
                     /translation="MHLMRDKPVGRFSPASLFRPESVVVIGADSTIGRQAMANLRASS
                     FKGDIQIVDEPERIAALPAAADLAVFAVQPTLDVMCALAAKGTFAAAVLCPSGALTEL
                     ARQTGVRMLGPDSFGIAVPGIGLNVSRAHLPLPEGRVALVSQSSALCRTVLDWAQPNG
                     IGFSHIVGIGGRADIGFGVVLDWLSRDPGTGAILLEIKELRSRRAFLSAARAAARLRP
                     VVAIRPGTLHADPSGATDRAFEAALRRAGVLCVDHLEDLLAAAETLARARPARSEALS
                     IVTNAVSAGQLAADAVLRNGMQLACDIVHVAPEASDQLAPYALVASECGGVLVVHVPA
                     GGADDTAIAALAACRDKVKLPLLVCAMGETTGAAHRQALAKAGIAVFATPEQAVRGFR
                     HLVQDRRNRAAARELPGSDVLSVASDRDTVRGLFAAIRRDGRLGTMQDEALAVLSAYG
                     IPVVPNRVAQSPADAADAAALLGFPAVVKLRQSVRPDARASGGLALDLHDGSEVAAAA
                     ERLGAVQLLVQHQAVRWRELRIHIVDDATFGPIINFGQGGTTADIVGDVAADLPPLNL
                     PLAHALVARTRAAATLGRFRDNPPANEAAVAEALVRISQLIVDFPEIAELDVNPLIVD
                     IDGVVAADAWIALREPGDRGGGLAISPYPAELVEHWIATDGERLVVRPIRPEDAEQHG
                     AFFHRLSPQDIRFRFFTAMRELSAEQTARLTQIDYDREMAFLAVREATGETVGVARLV
                     CENDRDGEFAIIVQQDMKGRGIASHLMRRLIDWARVRGLSNVIGQVLADNAPMLAFVR
                     H"
     gene            2452..3411
                     /locus_tag="VKQ27_14350"
     CDS             2452..3411
                     /locus_tag="VKQ27_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY90159.1"
                     /translation="MSDRSRWDPEMVAYQQAGEAIAAKFPPVKLELPLEPHRAVNDAI
                     TLARMVRGPDMAETTERWIAARGRRIYCRVHRPRTDRPLPVLVYFHGGGWVWGSVDTH
                     DCAARSLAAAGDVAAVSVDYALSPEAKFPQALEECAAVVRHIAREGGAWGLDTSRILL
                     GGDSAGGNLALATALLLRDTEGPALAGIVTAYPVCDSRLDTASYREFATGHVLTREKM
                     GFYWNAYVPHEADRAHPLAAPLRADLTGLPPVLVLLAELDVLRSEGEALVAKLRAAAV
                     PVETETYAGVLHGFFRATDGLQKARDAVARAGTWIRRVAHPPR"
     gene            3687..4118
                     /locus_tag="VKQ27_14355"
     CDS             3687..4118
                     /locus_tag="VKQ27_14355"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HLY90160.1"
                     /translation="MGQPSQPALSASVKAGVLTCDVASGWSLIFGSTRDLKCTYAADN
                     GHVEHYSGRINKYGVDVGYKAAGVIAWAVLAPTTDVGKGALAGTYGGVTGGAALGVGG
                     SANLLVGGSGQTISLQPLSVEGMSGINVAAGIAGIVLKREN"
     gene            4218..>4927
                     /locus_tag="VKQ27_14360"
     CDS             4218..>4927
                     /locus_tag="VKQ27_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLY90161.1"
                     /translation="MKHGLLDPSQLQRMVYETLDQSDDCVLILEKTADAADGLIVVSA
                     NDAFCRASGFSQDELLSQPLRTLAAEENGQSRCGELEGAAREMRAIRCELPFHRKDNG
                     VFWFGLHMMPARDSAPGCFVVLGRDITEARFIRQQQAAVQGLLAKVFLAVKAPVAIVA
                     HDGLIQMTNPALDELLGYPPGGLIGKNAMECNAPSMRPSLQAALERQITDGRDYMAAT
                     RLLRTDGREVAVELTSITV"
BASE COUNT          885 a         1565 c         1700 g          777 t
ORIGIN      
        1 atgccgcacg aaggcgagca tcggcgcgtt gtcggcgagt acctggccga tgacgttgga
       61 cagcccgcgc accctcgccc agtcgatcag cctgcgcatc aggtgagagg caatgcccct
      121 gcccttcatg tcctgctgca caatgatggc gaattcgccg tcgcggtcgt tctcgcagac
      181 caggcgtgca acgccgacgg tttccccggt cgcctcgcgc acggcaagga aggccatctc
      241 gcggtcgtag tcaatctgcg tcaggcgcgc cgtctgttcg gccgacaact cgcgcatggc
      301 ggtgaagaag cgaaaacgga tgtcctgggg ggacaggcgg tggaagaagg cgccatgctg
      361 ttcggcgtcc tcggggcgga tcggacggac caccaggcgt tcgccatctg ttgcaatcca
      421 atgctcgacc agctcggctg gataaggtga gatcgcgagg ccgccgcccc ggtcgccggg
      481 ctcacgcaga gcgatccatg cgtcggcggc gaccacgcca tcgatatcca cgatgagggg
      541 attgacgtcg agctcggcaa tctcggggaa atcgacgatg agctggctga tgcgcaccag
      601 cgcctcggca accgcagcct cgttcgcggg cgggttgtcg cggaacctcc cgagcgtggc
      661 ggcggcgcgg gtgcgggcga ccagggcgtg ggccaggggc agattgagcg gcggcagatc
      721 ggctgccacg tcgccgacga tatccgcggt cgtgccgccc tggccgaagt tgatgatcgg
      781 cccgaacgtc gcgtcatcga cgatgtgtat acggagctcg cgccagcgta ctgcctggtg
      841 ctgcaccaga agctgtacgg caccaagccg ttcggccgcg gcggcgacct cagagccatc
      901 gtgcaggtcg agcgcgaggc cgccggaagc gcgcgcatcc ggccgaacgc tctgccgcag
      961 cttcacgaca gcgggaaacc cgagcagcgc cgccgcatcc gcagcgtcgg ccggcgactg
     1021 cgcgacacgg ttcggtacaa ctggaatacc gtatgcgctc agcactgcca gcgcctcatc
     1081 ctgcatcgtg ccaagccggc catcacgccg gatcgcggca aacagaccac gaaccgtgtc
     1141 ccgatcggac gcaaccgaca gcacatcgct gcccggcaac tcgcgcgccg cggcgcgatt
     1201 gcggcggtcc tgcacaagat ggcgaaagcc tcgaactgcc tgctccggag tggcgaatac
     1261 cgcgatgccc gccttggcca acgcctgacg gtgagccgca ccggtggtct cgcccatggc
     1321 gcacaccagc agcggcagct tcaccttgtc gcggcacgcc gccagggcag cgatcgcggt
     1381 atcgtcggcg ccgccagcgg ggacgtgcac gaccagcacg ccgccacatt ccgatgccac
     1441 cagggcatac ggcgccagtt ggtcggacgc ctccggtgca acgtgcacga tgtcgcaggc
     1501 caattgcatg ccgttgcgca gcactgcatc cgccgccagt tggccagcgc tgacggcatt
     1561 ggtcacgatc gacagcgcct cgctgcgcgc tggacgggca cgcgccagcg tttcggcggc
     1621 ggccaacagg tcctccagat gatcgacgca gagcaccccg gcgcggcgca atgccgcctc
     1681 gaaggcgcga tcggttgcgc cactcggatc ggcgtgcagc gtgccgggtc ggatggccac
     1741 caccggacgc agtctggccg cggcacgcgc cgccgagagg aaggcgcgcc ggctgcggag
     1801 ttccttgatc tccagcagga tcgcgccagt gcctggatcg cgtgacaacc aatcgaggac
     1861 gacgccgaag ccgatatccg cacggccgcc aatgccgacg atgtggctga aaccaatgcc
     1921 gtttggttgc gcccagtcca gcacagtgcg gcacagagct gacgactgcg ataccaacgc
     1981 gacgcggcct tccggcagag gcagatgcgc tcgcgagacg ttcagcccga tgcctggcac
     2041 ggcgatcccg aaggagtccg gacccagcat gcgaacgccg gtttggcgtg ccagttccgt
     2101 caaggccccg gatgggcaaa gcaccgcagc ggcgaaggtg cccttggccg ccagcgcaca
     2161 catcacgtca agtgtcggct gcaccgcgaa taccgcaagg tcagcggcgg ccggcaatgc
     2221 ggcgatccgt tccggttcgt caactatctg gatgtcgccc ttgaagctgc tcgcccggag
     2281 attggccatc gcctggcgtc cgatggtgga atcggcgcca atgacgacga cagactcggg
     2341 gcggaacaga ctggctgggc taaagcggcc aacgggcttg tcgcgcatca ggtgcatggc
     2401 actggccatc ataagccggc aggatgaacc agcgaataag gggagagggc catgagcgac
     2461 cggtcgcgct gggatccgga aatggtggcg tatcagcagg ccggcgaggc catcgcggcg
     2521 aagttcccac cggtgaaact cgaattgccg ctggaaccgc atcgcgcggt gaatgatgcg
     2581 atcaccctgg cgcggatggt ccgcgggccc gacatggcgg agaccaccga acgctggatc
     2641 gccgcgcgcg ggcggcggat ctattgccgg gtgcatcgcc ctcgcaccga tcgtccgctg
     2701 cctgtgctgg tgtatttcca cggcggcggc tgggtgtggg ggtcggtgga cacacatgac
     2761 tgcgccgcgc gctcactcgc cgcagctggg gacgtcgcgg cggtcagcgt cgattacgcc
     2821 ctgtcacccg aggcgaaatt cccacaggcg ctggaggagt gtgccgcggt ggtgcggcat
     2881 atcgcccgcg aaggaggcgc ctgggggctc gacacgtcgc gcatcctgct gggcggtgac
     2941 tctgcgggcg gcaatctggc gctggcgacc gcgctgctgc tgcgggacac cgaagggccg
     3001 gcgctggccg ggatcgtgac ggcctatccg gtgtgcgaca gccggctcga cacggcgagc
     3061 taccgggaat tcgccaccgg gcacgtactg acgcgcgaaa agatgggctt ctactggaat
     3121 gcctacgtgc cgcatgaggc cgatcgcgcc catccgctgg cagcgccgtt gcgcgcggat
     3181 ctcaccgggc tgccgccagt tctggtgctg cttgccgagc tcgacgtgct gcggtccgag
     3241 ggcgaggcac tggtcgcgaa gttgcgcgcc gccgcggtgc cggtggagac agagacgtat
     3301 gccggtgtcc tgcacggttt ctttcgcgcc accgacggct tgcagaaggc tcgtgatgcg
     3361 gtggcgcggg cgggtacctg gatcaggcgc gtcgcccatc caccacgcta gagcgtgatg
     3421 accagaggcg gaaccgccgg aggtctgaat cacgtgataa aacaaaaggc cagagcgaga
     3481 tcagcgcgtc cttcagcgct catcccgctg tagggcagtg cgggcacccg gatcgttcaa
     3541 tctgtgccgc aaacaggcta gtacgacgca ggggatggat tacgcgccgg acatgtgtcg
     3601 gcggggcatc catttcgaca gtccaacagg agcagctcat gcattggaaa gcagccttga
     3661 ccgccgccgc aatagtggcc gttgcaatgg gacaaccgag ccagccggcg ctgtcagcgt
     3721 ccgtgaaggc cggcgtgctg acctgtgacg tagcgagcgg atggagcctg atcttcgggt
     3781 caacgcgcga cctcaagtgc acgtatgccg cggacaacgg gcatgtcgaa cactactcag
     3841 gccggattaa caagtatggc gttgacgtcg gctacaaggc tgccggggtg atcgcctggg
     3901 ctgtgctggc accgaccacc gatgttggga agggcgccct cgcggggacg tatggcggcg
     3961 tgacgggcgg cgcggcactc ggcgtcggtg gcagcgcgaa cctgctggtc ggcggttccg
     4021 gccagacgat ctcgctgcag ccgctcagcg tggaagggat gagcggcatc aatgtcgccg
     4081 ctggcatcgc cggtatcgtg ctgaagcggg agaactagcg ccagatctca ggattgaccc
     4141 actgttgagc aggtaattag ccggggatta aggcggtccg tcgcatgatg tctgaccgag
     4201 ccaatgggat cgggaggatg aagcacggac tgctagaccc ctcgcagctt cagcgcatgg
     4261 tctacgagac gctggatcaa tccgacgact gcgttttgat tttggagaag acggctgatg
     4321 ccgcggacgg cctgattgtc gtctcggcga atgacgcatt ttgccgggcc agcggattca
     4381 gccaggatga gctgctcagc cagccgttgc gcacgctggc ggcggaggag aacggacagt
     4441 cccgctgcgg cgaactggag ggcgccgcgc gggagatgcg cgccatccgt tgcgagttgc
     4501 cattccaccg caaggacaat ggcgtatttt ggttcggctt gcacatgatg cccgcgcgcg
     4561 acagtgcgcc gggctgtttc gtcgtcctcg ggcgcgacat caccgaggcg cgatttatca
     4621 ggcaacaaca ggcggcagtt caggggttgc ttgcaaaggt attccttgcg gtgaaggcac
     4681 ccgtcgccat cgtcgcgcat gacggattga tccagatgac caatccggcg ctcgacgaac
     4741 tgcttggata tccgcccggc ggcctcatcg gcaagaatgc catggaatgc aacgcaccaa
     4801 gcatgcgccc gtcgctgcaa gcggcacttg aacgccagat cacggacggc cgcgattata
     4861 tggcggcaac caggttgttg cgtacggatg gccgcgaggt cgcggtcgaa ctcacgtcga
     4921 ttaccgt
//
LOCUS       DATGEM010000451         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_505461, whole genome shotgun sequence.
ACCESSION   DATGEM010000451 DATGEM010000000
VERSION     DATGEM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505461"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="VKQ27_14365"
     CDS             complement(<1..1498)
                     /locus_tag="VKQ27_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLY90162.1"
                     /translation="MLGRIRLQSDLGVVAVLVATILLMIVPLSTTLVDLLLAFNLGIS
                     VLLLMVAIYLQSPVQFSTLPAVILIATVFRLSLSIAVTRLVLLQADAGAIISTFGNFV
                     VSGSIAVGLVIFLIITIVQFIVITKGAERIAEVAARFTLDALPGKQMAIDADLRAGEL
                     DQQAARTRRRDLERESQLYGAMDGAMKFVKGDAIAGLVIICVNLIGGLAVGTLQNGMS
                     LAEAAHTYSILTVGDGLMAQIPALFVSITAGAVVTRVSGGDADSLGAEIALQLGKNPR
                     ALWLAAVIAALLGCIPGFPTLIFLATAGGIGFMAHRSGRRQRAAAEAKPARSAAPALP
                     ARVQLHLDAPLAAQLGVERLTISIARNSATLAKELGVPVPTAEVISTPSGDGLMRLEL
                     DGVPIAEAAIPPDRLLLRDDAENAELVGAAVEPGRPIPGRDVTLWVQSSQRQVLVSSG
                     IGFLDPIDALAQCVPAALRRHASRLIGVQETRQLLTGAEAHWGELVREA"
     gene            complement(1501..1851)
                     /locus_tag="VKQ27_14370"
     CDS             complement(1501..1851)
                     /locus_tag="VKQ27_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90163.1"
                     /translation="MTPEVLDLLHCLGFIYLRHGQPYRAVVLLIIAAQYAPERADILR
                     TLAAALVAAHMGEQALDVLRRLAALDSAEADHPMMRLTRARALLLLGRVEEARRAFRP
                     SPTALPDKRIEKAA"
     gene            complement(1858..>2151)
                     /locus_tag="VKQ27_14375"
     CDS             complement(1858..>2151)
                     /locus_tag="VKQ27_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90164.1"
                     /translation="AEQLDPVEALAALTDEPGDIGSNGLADQLLDYVRPRPRHPEALT
                     QPRIIPLLGAAADLLRNAASQEDTIIALGGTALEQELRQHRMLAERRASLLEG"
BASE COUNT          400 a          727 c          675 g          349 t
ORIGIN      
        1 gagcttcgcg caccaactca ccccaatgcg cttcggcgcc ggtcagcaat tgacgggttt
       61 cctggacgcc aatgagccgg ctggcatgac ggcgcaacgc tgccggtacg cattgtgcca
      121 atgcgtcgat cgggtcgagg aaaccgatgc cgctgctcac cagcacctgg cgctgcgaac
      181 tctgcaccca cagcgtcacg tcccgtcccg gaatcggccg gccaggctcg acggcagcgc
      241 ccaccagttc ggcgttttcc gcatcgtcgc gcaacagtag ccggtccggg ggaatcgctg
      301 cttcggcaat cggcacgccg tcgagttcca ggcgcatcag tccatcgccc gacggcgtcg
      361 aaatcacttc ggccgtcggc accgggaccc ccagttcctt tgccagcgtg gcactgttac
      421 gggcgatcga aattgtcagc cgctcgacac caagttgcgc tgccagcggt gcatcgagat
      481 gtagttgcac acgggcgggc aatgccggcg ccgccgaccg ggcgggtttc gcttcggccg
      541 cggcacgctg ccggcggccc gaccgatggg ccataaagcc gataccgcct gcggttgcca
      601 ggaagatcag cgtcgggaag cccgggatac accccagcag tgccgcgatc accgccgcaa
      661 gccacagcgc gcgtggattt ttgccgagtt gcagcgcgat ctcggcacca aggctgtccg
      721 catcgccgcc gctcaccctg gtcaccactg cccccgcggt gatcgagacg aacagcgcgg
      781 gaatctgcgc catcagcccg tcgcccaccg tgaggatcga ataggtgtgc gctgcctcgg
      841 caagcgacat gccattctgc agcgtgccga ctgccagtcc gccgatgagg ttgacgcaga
      901 tgatgacgag ccccgcgatg gcatcgccct tcacgaactt catcgcgccg tccatcgcac
      961 catagagctg actttcgcgc tccaggtccc gccgccgcgt gcgcgctgcc tgctgatcaa
     1021 gttctcctgc tcgcaagtcg gcgtcgatcg ccatctgctt gccgggtaac gcgtccaacg
     1081 tgaagcgcgc ggccacctcg gcaatgcgct ccgccccttt ggtgatgacg atgaattgca
     1141 cgatggtgat gatcaggaag atcaccaggc cgacggcaat gctgccggac accacgaaat
     1201 tgccgaaagt gctgatgatg gcgcccgcgt ccgcctgcaa cagaaccagg cgcgtgacgg
     1261 caatcgacag cgaaagtcgg aacaccgttg cgatcaggat gaccgcgggc agcgtagaga
     1321 actgcaccgg gctttgcagg tagatcgcca ccatcagcag cagcacgctg atacccagat
     1381 tgaacgccag cagcaaatct accagcgttg ttgacagcgg cacgatcatc agcaggatag
     1441 tggcgaccag caccgccacc acgccgagat cggattgcag gcggatgcgc cccagcaagc
     1501 tcaggcagcc ttttcgatgc gcttgtccgg cagcgcggtt ggcgagggac gaaacgcccg
     1561 gcgggcttcc tcgacgcggc cgagcagcag aagcgcacgt gcacgggtca ggcgcatcat
     1621 cgggtgatca gcttcagcgg aatcgagcgc ggccaatcga cgcaggacat cgagcgcctg
     1681 ttcccccata tgtgctgcaa ccagagccgc cgccagcgtg cgcaggatgt cggcgcgctc
     1741 cggcgcatac tgcgcggcaa tgatcagcag cacgaccgca cgatacggct ggccatgccg
     1801 caggtagatg aagcccaggc agtgcaggag atcgaggacc tctggtgtca tggctgccta
     1861 tccttccagc agcgaggcac gccgttcggc aagcattctg tgctgccgca gctcctgctc
     1921 gagcgccgtg ccgccgaggg cgatgatggt gtcttcctga ctggcggcgt tacgcagcaa
     1981 atccgccgcg gcgccaagca gcgggatgat ccgcggctgc gtcagcgctt ccggatgccg
     2041 cgggcgtggc ctgacataat ccagcaactg gtcagccagt ccattcgatc cgatgtcgcc
     2101 tggttcgtcc gtcagcgccg caagcgcctc gaccgggtcg agttgctcgg c
//
LOCUS       DATGEM010000452         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_123325, whole genome shotgun sequence.
ACCESSION   DATGEM010000452 DATGEM010000000
VERSION     DATGEM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123325"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3013)
                     /locus_tag="VKQ27_14380"
     CDS             complement(<1..3013)
                     /locus_tag="VKQ27_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY90165.1"
                     /translation="MKFGIQRRILTVLVGILLLATALEAVLASYYTNRQSQQAAFAAL
                     GRQLSAWQSALQITTWQLRGVALATMGDVAVLNQLAELMALEFNVNDPARVQVVPEMA
                     RALAYRKTVSLNRLQLALRTGGFSSIAVYMRGKLSHYVSESAAGMTVTNAKGSEVWVA
                     AAADAQGNLPFQSWPAWSEQQVPALEGAPAPAPSRPSVDFVFPREGQLMIEIAVPIQA
                     EVDDSMTEAERHPVVRFFSELRIAGAVPDNSRNASVNGLPDNPPRIVATVVFRKAIDS
                     TGPDILARQAGSSPTLLSLDGAHRQQLSKLDLIPPDMLAQVQTGLSTAPPRVIQRTVT
                     AGQGSFYVALLPWQFEGRPRLILSLAAPREGTLENIRQTVAAIVLVAGVILLLSVAVG
                     IWWVKKFVDPIIRLTSAVQEISSRNRLDDALQPGGPLARRPALPSLPAIDIKAPDEVG
                     ALARAFTDMTERLARQERDLISANADMAAVLARASAILDNIPDLAWVKDIEGRYIAAN
                     SVLARTLGLAHANEIIGKTDFDLSPREIAEGYRSADEEIIASGERKRLEELAWRPDGS
                     TFWVETIKTPLRDANGRIVGTVGIARDVTERRQAEREREARQLAEAANRAKSEFLANM
                     SHEIRTPMNAIIGMSSLALTSGLSPRQYNYINNVHRAAQLLLGILNDILDFSKIEAGK
                     LQIDAVAFDLRDVMDNLANLARQQAEEKGLELVFVEPPELPTRLVGDPLRLGQVLVNL
                     TNNAVKFTERGTITVSIDVLERSADGVLLRFGVRDTGPGISLEEQGRLFQPFSQVDAS
                     TSRRFGGTGLGLAICRQLVRLMGGAIGIDSVPGRGSHFIFTARFGLQPGSATSSAAQR
                     LVPLSGARVPPDAARGADARGTEREETPHDYGVRLAGVKILLVEDNFYNQELAVELLR
                     GAGIDVTLAADGRQALELLERQRFDGVLMDCQMPVMDGYEATRLLRQRPDLRDLPVIA
                     MTANAMVGDRDKVLAAGMNDHIVKPINVEEMF"
     gene            complement(3010..4152)
                     /locus_tag="VKQ27_14385"
     CDS             complement(3010..4152)
                     /locus_tag="VKQ27_14385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY90166.1"
                     /translation="MIEAAAAAFDRSQTAYKIELYNSNFKNHADWVQSIAMTGTLPCM
                     IEVDGPFVAAFAWRGYLRTLDQFVPREMRDDLLPSIISQGTYGGHLYSLGQFESGLGL
                     WANRHYLAAAQVRIPTVARPWTLAEFEQAMDRLARRGGVDYALNLSVYVDTNESYSFA
                     YSPILQGFGGDLVDRNTGLARGVLDGPQSVSAMTRFQYWFKRGWTRAVFDRNNDFARG
                     KTALSWTGHWKYIDYRKALGKDLILLPLPDFGHGIKTGTGAWSWGITSACPSPDGAWA
                     FLAYLMSANEILRVTGINGALPARKSALAQSPLYGAHGPLNTFAQQIMSGAGVPRPAT
                     PAYGTISKAFSGAVRAIIAGADVQTALRRAAAAIDEDIAANHGYPR"
BASE COUNT          800 a         1520 c         1553 g          850 t
ORIGIN      
        1 cgaacatttc ctcgacattg atcggcttga cgatgtgatc gttcatgccc gcggccagca
       61 ccttgtcccg gtctccgacc atggcgttgg ccgtcatggc aatcaccggc aggtcccgca
      121 ggtccggccg ctgccgcagc aggcgcgtcg cctcgtagcc atccatgacc ggcatttgac
      181 agtccatcag cacaccgtcg aaacgctgcc gttcgagaag ttcgagcgcc tgccggccgt
      241 cggcggcgag ggtgacgtcg atcccggcgc cgcggagcag ttcgaccgcg agttcctgat
      301 tgtagaagtt gtcctcgacg aggaggattt tgactccggc cagtcgcacc ccgtagtcgt
      361 gcggcgtctc ctcgcgctcc gtgccgcgcg cgtccgcgcc tcgtgccgcg tctggcggca
      421 cgcgcgcgcc ggaaagcggg acgagccgct gcgccgcgga ggacgtcgcg ctcccgggct
      481 gcaggccgaa gcgcgcggtg aagatgaagt ggctgccccg gcccggaaca ctgtcgatgc
      541 cgatggcgcc gcccatcagg cgtaccagct ggcggcagat cgccagcccc aggccggtgc
      601 cgccgaagcg gcggctggtc gaggcgtcga cctgtgaaaa cggctggaac aggcgcccct
      661 gctcctcgag actgatgccg gggccggtgt cacgcacgcc gaagcgcagg agaacaccgt
      721 cggcactccg ttcgagcacg tcgatgctga cggtgatcgt gccgcgctcg gtgaacttga
      781 cggcgttgtt ggtcaagttg accaatacct ggcccagccg caacggatcg cccaccagcc
      841 gtgtcggcag ctccggcggt tcgacgaaga ccagctcgag ccctttttcc tccgcctgct
      901 gccgggccag gttcgcaagg ttgtccatta cgtcgcgcag gtcgaacgct accgcgtcga
      961 tctgcagttt gcccgcttcg atcttggaaa aatccaggat gtcgttgagg atcccgagca
     1021 gcagctgcgc cgcgcggtgc acgttgttga tgtagttgta ctgccggggg ctgaggccgc
     1081 tggtcagcgc cagggaggac atgccgatga tggcattcat cggcgtgcgt atttcgtggc
     1141 tcatattggc gaggaattcg ctcttggccc ggttcgccgc ttcggccagc tgccgtgcct
     1201 cacgttccct ctcggcctgc ctgcgctcgg tcacgtcgcg cgcgatgccg accgttccga
     1261 cgatccggcc gtttgcgtcc cgcaacggcg tcttgatcgt ttcgacccag aaggtgctgc
     1321 catcgggccg ccaggccagc tcctccaacc gctttcgctc gccggacgcg atgatttcct
     1381 cgtccgcgct ccgatagccc tccgcaatct cccttgggct caggtcgaaa tcggtcttgc
     1441 cgatgatctc gttcgcgtgc gccaacccca gggtccgggc cagcaccgag ttcgcggcaa
     1501 tgtagcggcc ctcgatatcc ttgacccagg ccaggtccgg aatgttgtcg aggatggccg
     1561 aggcgcgggc gaggacggcg gccatgtcgg cattcgcgga aatgagatcc cgttcctgcc
     1621 gggcgagcct ttcggtcatg tcggtgaagg cccgggccag cgcaccgacc tcatcggggg
     1681 ccttgatatc gatggccggc aggctcggca gcgcgggacg ccgggcgagc ggccctcccg
     1741 gttgaagggc gtcgtccaaa cggttccggg agctgatttc ctggaccgcg gaagtcaggc
     1801 ggatgatcgg gtcgacgaac tttttcaccc accatatgcc caccgcgacg ctcagcagga
     1861 ggatcacgcc ggcgaccagc acgattgccg cgaccgtttg ccggatgttc tcgagagtgc
     1921 cctcgcgcgg cgccgccaga ctgaggatca agcgtggccg gccctcgaat tgccacggga
     1981 gcaaggcaac atagaatgac ccctgccccg cggtgacggt gcgctgaatg acgcggggcg
     2041 gcgccgtgga aagtccggtc tggacttgcg ccagcatatc gggcgggatc aggtcgagct
     2101 tgctgagctg ttgtcgatga gcgccgtcga gcgacaacaa ggtcggcgag ctgccggctt
     2161 gtcgcgcgag gatatccggt cccgtgctgt cgattgcctt gcggaacacc acggtggcga
     2221 cgatccgcgg cggattgtcg ggtaagccgt tgaccgatgc gttgcgggag ttgtccggca
     2281 ccgctcccgc gatccggagc tcggagaaga atcggaccac cgggtggcgc tctgcctcgg
     2341 tcatcgagtc atcgacctcg gcttgaatgg gaacggcgat ctcgatcatc agctgcccct
     2401 cccgagggaa gacgaagtcg acgctcggcc tcgaaggcgc cggcgccggg gcgccttcga
     2461 gcgccggaac ttgctgttca ctccaggcag gccaactctg gaagggcagg ttcccctggg
     2521 catccgccgc cgccgcgacc cagacctcgc tgccctttgc attcgtcacc gtcatgcccg
     2581 ccgccgattc ggacacgtaa tggctcagct tgcctctcat gtacacggcg atgctcgaga
     2641 aaccgccggt gcgcagcgca agctggaggc ggttgaggga cacggttttg cgataagcga
     2701 gggctctcgc catttcaggg acaacctgca ccctggccgg atcattgacg ttgaactcga
     2761 gtgccatgag ctcggccaat tgattgagga ccgcgacatc gcccatggtc gcaagcgcca
     2821 cacccctgag ttgccaggtc gtgatttgca gtgcgctctg ccaggcagac agctgtctgc
     2881 cgagggcggc gaaggcggct tgctggctct gccggttggt gtagtaggag gcgagaaccg
     2941 cctccaatgc ggtggcgagc agcaggatcc cgaccaatac cgtgagaatc cgccgctgga
     3001 tgccgaactt catcgcggat atccgtgatt ggcggcgata tcctcgtcga tcgcggcggc
     3061 agcgcggcgc agcgccgtct gcacatcggc accggcgatg atggctctca ccgccccgga
     3121 aaatgccttg ctgatcgtgc catacgccgg cgtcgcggga cgcggcacgc cggcgccgct
     3181 catgatctgc tgtgcgaagg tgttcagcgg cccatgggcg ccgtacagcg gcgactgcgc
     3241 aagcgccgac ttgcgggccg gcagcgcgcc gttgatgccg gtcacgcgca ggatttcatt
     3301 ggccgacatc agataggcga ggaacgccca ggcgccgtcc gggcttggac aggcgctggt
     3361 gatcccccaa ctccaggcgc ccgtgcccgt cttgatgccg tgcccgaaat ccggcaaggg
     3421 cagcagtatg agatctttgc ccagcgcctt gcggtagtcg atgtatttcc agtgccccgt
     3481 ccaagacaac gcggtcttgc ctcgcgcgaa gtcgttgttt cggtcgaaca ccgcacgagt
     3541 ccagccgcgc ttgaaccagt actggaaccg cgtcatcgcg ctcacggatt gcggtccgtc
     3601 gagcacgcct cgcgccaatc cggtgttgcg gtcgacgagg tctccgccga acccttgcaa
     3661 tatgggcgaa tacgcgaagg aataggactc gttggtatcg acatagacgg acaaattcag
     3721 ggcgtaatcc acgccccccc gccgcgcgag ccggtccatg gcctgctcga attccgcaag
     3781 cgtccaaggg cgggcgacgg tgggaatgcg aacctgggcc gcagccaggt agtggcggtt
     3841 ggcccagaga cccaaacccg attcgaactg tccgaggctg tagaggtgcc cgccgtaggt
     3901 gccttgggaa atgatcgaag gcaacaggtc gtcccgcatt tcacggggca cgaattggtc
     3961 cagtgtccgc agatatccgc gccacgcgaa agccgcgacg aacgggccgt cgacttcaat
     4021 catgcaaggc agcgtgccgg tcatcgcgat gctttgcacc caatctgcat gatttttgaa
     4081 gttggaatta tacagctcga tcttgtaggc ggtttgggag cggtcgaatg ccgcggcggc
     4141 ggcttcgatc aagcgagcct cgtcgccgtg aaagaccgag cgcatcccga cggtaatggt
     4201 cgtgggggac gacgaggcgc cgatcgcggg agcgccgcac atggcgagca gcagcgcggt
     4261 gccgccgatg caggcgacga gccgattgtg atacgagctc ttcatcggcg tccccgcgga
     4321 tgatccgtgc cagtgtgtcg gtgcggctct gcggccgcgt catcgcgaaa cgactcgatt
     4381 atagcgggtt atgccgatgc ggtctttcgc acgacaccgt ggaggattcc ccgtacctct
     4441 cgtcggatct catcagcatg agttcatcgc gagaccggcg gggtgccaag cgcagtccac
     4501 atccaaacgt ggcgttggat gtctctcggg ttccgttcca aacgtggtat ggatgccatt
     4561 caggctcagt cgcgaatcag cagcgcgacg ggaaattgat cgaagagctc ccccgggctc
     4621 accacgcccg aagcggaggc gcccgtgaaa tgattcctcc agttgcctcg cggcagcggc
     4681 agacgcgtat ctccccagtc gccggcgacc gtcatggtga acc
//
LOCUS       DATGEM010000453         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_123393, whole genome shotgun sequence.
ACCESSION   DATGEM010000453 DATGEM010000000
VERSION     DATGEM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123393"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VKQ27_14390"
     CDS             complement(<1..1309)
                     /locus_tag="VKQ27_14390"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY90167.1"
                     /translation="MIATLTRSVYGPKSERFLEADQRQMDLGLSDLSSIPVEPEAKPE
                     PKPPHEPRPRRPARRNIGSLPGHLPREDIFLEPDATECPCCRGALHKIGEDLSEMLDH
                     VPAILRVKRFHRPRYGCRACESVVAQAPAPERPVANGLPTTGLLAHVAVSKFAWHIPL
                     HRQTKMLAASGISIDRSTLVQWIERAAWWLKPLHALLAETVFACPKVFCDDTPLPVLD
                     RARTRTRIGRIWCYAVDDRPWQGTRPPAVVYLFAEDRRGRHVSEHLDSFTGVLQVDAY
                     AGYDALTRPGRKAGPITLAYCLAHARRRFFDLHKGGNQPVATEAVRRISDIYEIEAGI
                     RGKQPDMRLAVRQRETRPLMAEFKSWLMARLEEISVKSKLAEAIRYTLGHWDGLTSFV
                     DDGRIEVDSNTVERQIRAIALGRRNALFAGSTAGGERWAILSSL"
     gene            complement(1445..1834)
                     /gene="tnpB"
                     /locus_tag="VKQ27_14395"
     CDS             complement(1445..1834)
                     /gene="tnpB"
                     /locus_tag="VKQ27_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188732.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HLY90168.1"
                     /translation="MILGVPVRPGLRIMLASQPVDFRKGMDSLFALVGQALSADVFAG
                     DVFIFRSKRLDRLKILMWDGSGLCLLTKRLEAGAFTWPPVRDGAVSLSAAQLRLLLTG
                     IDWTQLGTAGSASSRADTVPGKLGNSV"
     gene            complement(1831..2235)
                     /locus_tag="VKQ27_14400"
     CDS             complement(1831..2235)
                     /locus_tag="VKQ27_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY90169.1"
                     /translation="MEVITGTVRRRRWTAEEKASLVAESFRPGINVSELARRFGVNRG
                     LLQTWRRRATQSVENAAAAFVPIRVEEPGFSVASNAKPAVAVSQSSESLSPGQQEKGT
                     IEIEAGGMHIRVHGSVDLEALRTVLARLERRR"
BASE COUNT          443 a          852 c          818 g          445 t
ORIGIN      
        1 tcaacgacga gaggattgcc cagcgttcgc cccccgccgt cgagccggcg aacaatgcgt
       61 tgcgtctgcc cagcgcaatg gccctgatct ggcgctccac cgtgttgctg tccacctcga
      121 tgcgtccgtc gtcgacgaac gacgtcaggc cgtcccaatg accgagcgta tagcggatcg
      181 cttcggcgag cttggacttg accgagatct cctcaagccg cgccatcagc caggacttga
      241 actcagccat cagcggcctg gtctcgcgct ggcgcaccgc caggcgcatg tcgggttgct
      301 tgccgcgaat gccggcctcg atctcgtaga tgtcggagat ccggcgcacc gcctcggtgg
      361 ccacgggctg attgccgccc ttgtgcaagt cgaagaaccg ccgccgtgca tgtgccaggc
      421 aataggcgag cgtgatggga ccagccttgc ggccagggcg cgtgagtgcg tcgtagccgg
      481 cataggcgtc gacctgcagc acgccggtga agctgtcgag atgctcgctg acgtgtcgtc
      541 ctcgtcggtc ctcggcgaac agatacacca ccgccggtgg ccgggtccct tgccagggtc
      601 ggtcgtccac cgcgtagcac cagatgcgcc cgatgcgggt ccgcgtgcgg gcgcggtcca
      661 gcaccggtag cggcgtgtcg tcgcagaaca ccttggggca ggcgaacacg gtctcggcca
      721 gcagggcatg cagtggcttc agccaccaag cggcccgctc gatccattgc accagcgtcg
      781 agcggtcgat ggagatgccg gaggcggcga gcattttggt ctgccggtgc aacgggatgt
      841 gccaggcgaa cttcgagacc gcgacatggg ccagcaagcc ggtcgtcggc agtccgttgg
      901 cgaccggacg ctccggtgcg ggtgcctgcg cgaccacgct ctcgcaggca cggcagccat
      961 agcggggccg gtggaagcgc ttgacccgca ggatggccgg cacgtggtcg agcatctcgc
     1021 tgaggtcttc gccaatcttg tgcagcgcgc cgcggcagca cgggcattcg gtggcgtctg
     1081 gttcgagaaa gatgtcctcg cgcggcaggt gacctggcag gctgccgatg ttgcgccgcg
     1141 cgggccgacg cggacggggc tcgtgaggcg gtttgggttc cggcttggcc tcgggttcga
     1201 ccggaatgct cgacaggtcg ctgagcccga gatccatctg cctctgatcg gcctcgagga
     1261 accgttccga ttttggtccg tagacggatc gcgtgagcgt cgcgatcatc gtgcgcagcc
     1321 ggtcgatctc ctcctgctga tcgacaatga tgtcgatcag ggcatcgggg tctctcggca
     1381 atgtcggaac atccttcgcc acgccgccag tatgaagcgg ggattcgccg cttcgctagg
     1441 cgcatcaaac agaatttcca agttttcctg gcaccgtatc cgctcgcgag gatgcgctcc
     1501 cggcagtccc caactgcgtc cagtcgatgc cggtgagcag aagccgcaac tgcgcggcgc
     1561 tcagcgacac cgcgccgtcg cggacagggg gccacgtgaa cgcgccggct tcgaggcgtt
     1621 tggtcagcag gcagagaccg gatccgtccc acatcaaaat cttcaggcgg tccaatcgct
     1681 tggagcggaa tatgaagacg tctccagcaa atacatccgc cgacaaagcc tggccgacca
     1741 acgcaaaaag gctgtccata cctttgcgaa aatcaactgg ctgagacgcg agcatgatgc
     1801 gcagaccggg ccgcacggga acgccgagaa tcaacgccgg cgctccagtc tcgccaggac
     1861 cgtccgcagg gcttccaggt cgaccgaacc atgcacgcgg atgtgcatgc cacccgcttc
     1921 gatctcgatc gtacccttct cctgctgccc tggcgacaaa ctttccgaag actgggacac
     1981 cgcgacggcg ggcttcgcgt tgctcgccac gctaaagcca ggctcctcga cgcggatcgg
     2041 cacgaacgca gccgcggcgt tctcaaccga ttgcgtcgcg cgacggcgcc aagtctgcag
     2101 cagaccccgg ttcaccccaa atcgccgcgc cagttccgat acgttgatgc cgggccgaaa
     2161 gctctcagcc accagggacg ctttctcctc tgccgtccaa cgccgccgac gcactgtccc
     2221 ggtgatcacc tcaatccgcc gatactctcg gttgtcgcgg cgagcaaacc ccgccacgtc
     2281 gctcagaatg ctgtccatgc catctctcgc gcgacctgtg tgcgcagaga gcttggccag
     2341 ttacgcaccg gattcccagg tggcgcactc gcgccgctta cgctgtttcg ctagcgctgg
     2401 tggtctattt cgcggcgcag tacctgctgt tccccgaaga gcttgcgcac aagacgctgg
     2461 acgaagaagc ggcggaggtt gccgccgctc tggcagctgg cgagaatcct gcaacgtggg
     2521 accaatacgt gcgctatccg caggcgtatg cctaccgc
//
LOCUS       DATGEM010000454         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_958421, whole genome shotgun sequence.
ACCESSION   DATGEM010000454 DATGEM010000000
VERSION     DATGEM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958421"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VKQ27_14405"
     CDS             complement(<1..504)
                     /locus_tag="VKQ27_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90170.1"
                     /translation="MKVGMFINTQFPEGDNVAARVPELVEQVRLARESGFSSLLFPHH
                     YLTAPLQMLQITPLMGYLLREAQGMIVGGNILLLPLLNPVHVAEESATLDVLSGGKFI
                     LGVGLGYREGEFDAFGISLRHRAPRFAESIALMRRLWTEDRVHFEGRFYQVKDHGISL
                     KPLQKGGP"
     gene            complement(601..1554)
                     /locus_tag="VKQ27_14410"
     CDS             complement(601..1554)
                     /locus_tag="VKQ27_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90171.1"
                     /translation="MNRAIPVIEGEALTKTFRSRGRIVHAVQGVSLGVSAGEALGIVG
                     ESGCGKTTTARLLLRLEEPSGGAIRFLGDDITARQGHALLHFRARAQLVFQNPFDALN
                     PRFTIRRALTEPLRNFAIPRSEHAERIAAVMQRVRLVPAEAWLDRRPHELSGGQLQRV
                     VLARALLPQPKLIVADEPVSMLDVSVRAGILNVLRAARDELGLAAIYISHDLALIRYV
                     CERTLVMYLGRMVEEGPTAALIAAPLHPYTQALVAAVPVPRVQQDRDSLPIRGSLSEA
                     PDSSAGCPFRVRCPHAFERCDAEAPALRDVAAGRRVACHLI"
     gene            complement(1551..2525)
                     /locus_tag="VKQ27_14415"
     CDS             complement(1551..2525)
                     /locus_tag="VKQ27_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90172.1"
                     /translation="MHYATGGGTVRAVDGVDLDVPAGSIVGLVGESGCGKSTLGRALM
                     GVLPGSGRIAGGSITFEGKDLARLSERELRALRWRRLSFVPQTAMNALDPVQRVGAQM
                     REVLRERGGLSRTVAHGRAETLMRLVGLDPKCLMDYPHQFSGGMRQRASIALALALEP
                     ALVIADEPVTALDVIVQRQVLDVLRDLQRRLNLAMILVTHDMAVVAYACDRAAVMYAG
                     KIVESGPVGAVLEAPFHPYTMGLTHAFPDLRSKEVALVPIEGAPPSLLEPPKGCRFAE
                     RCPFVQNRCRIESPPLVQVADDHLTACWRSDAAVALREEAARPETWAA"
BASE COUNT          554 a          966 c          782 g          426 t
ORIGIN      
        1 cggaccgcct ttctgcagcg gcttcaggct gatgccgtgg tccttcacct gatagaagcg
       61 cccctcaaaa tgcacacgat cctcggtcca cagacgccgc atcagcgcga tgctctccgc
      121 gaagcgcggc gcacgatgac gcagcgaaat gccgaacgcg tcgaactcgc cctcgcgata
      181 acccagaccc acgccaagga tgaacttgcc gccagacagc acgtcgaggg tcgcggactc
      241 ttcagccaca tgcacgggat tgagcagcgg cagcagcaga atattgcccc ctacgatcat
      301 gccctgcgcc tctcgcagca aataccccat caacggcgtg atctgcagca tctgaagcgg
      361 cgcggtgaga tagtgatgcg ggaataatag cgacgagaag ccgctctcgc gcgcgagccg
      421 cacctgttcc actagctccg gcacgcgcgc agcaacattg tcgccttccg gaaactgcgt
      481 gttgatgaac atcccaactt tcatacggcc tcctgtcatt gcgatccccg caatggccgg
      541 gggaagcaat cccgaccgag tttgcttcct cgccccgctc cccgcaatca cgcgacggca
      601 tcaaatcaga tggcacgcca cccgtcgccc cgccgcgacg tcccgcagcg ccggcgcctc
      661 ggcgtcgcag cgttcaaatg cgtgcgggca gcgaacgcgg aatggacagc ccgcggaact
      721 gtccggcgcc tcgctcaggc tgccccggat cggcaagctg tcgcggtcct gctgcacccg
      781 cggcacggga accgccgcga caagcgcctg tgtatacgga tgcagcggcg cagcgatcag
      841 cgcagccgtg ggtccctcct ccaccatgcg gccaagatac atgaccagcg tgcgctcgca
      901 gacgtaacgg atcagcgcca gatcgtggct gatatagatc gccgccaatc caagctcatc
      961 gcgtgcggca cgcagcacat tcaggatgcc ggcccgcaca gacacatcca gcatcgatac
     1021 cggttcgtcc gccacgatca gcttcggctg aggcaggaga gcccgtgcca gaaccacacg
     1081 ctgcaactgc ccaccagaca attcatgcgg acggcgatca agccaggcct cggccggcac
     1141 aagccgcacg cgctgcatca ccgcggcgat ccgctccgcg tgctctgaac gcggaatggc
     1201 gaagttgcgc agcggctcgg tcagcgcgcg ccggatggta aagcgcgggt tcagcgcatc
     1261 gaacgggttc tgaaacacaa gctgcgcacg cgcacgaaaa tgcagcagcg catggccctg
     1321 gcgcgccgtg atatcatcac cgagaaaacg gatcgccccg ccactcggtt cttccaggcg
     1381 taaaagcagg cgcgccgtcg tcgtctttcc gcatccagat tcgcccacga tgcccagcgc
     1441 ttcgccggcg ctcacaccga ggctcacgcc ctgcaccgca tgcacaatgc gaccgcgcga
     1501 ccgaaaggtc ttcgtgagtg cttcgccctc gatcaccggt attgcgcggt tcatgccgcc
     1561 caggtctccg gccgtgcggc ctcctcgcga agagcaacgg cagcgtcgct gcgccagcat
     1621 gctgtcaggt gatcgtccgc cacttgaacc aagggcggcg attcgatgcg gcaccggttc
     1681 tgcacgaatg gacaacgctc ggcgaagcgg catccctttg gcggctccaa cagcgacggc
     1741 ggcgctccct cgattggcac cagagcgact tccttcgagc gaaggtcggg gaaggcgtgc
     1801 gtcaaaccca tcgtataggg gtgaaagggc gcttcaagca ccgcaccgac agggcccgat
     1861 tcgacgatct tcccggcata catcacggcg gcgcggtcgc aggcataggc gaccaccgcc
     1921 atgtcgtgcg tcaccaggat catcgccagg ttcaaccgcc gttgcagatc acgcagcacg
     1981 tccagcacct gtcgctgcac gatcacatcg agcgccgtga ccggttcgtc ggcaatcacc
     2041 agtgccggtt ccagcgccag agccagcgcg atcgatgcgc gttgccgcat gccgccgctg
     2101 aactggtgcg gatagtccat caggcacttc ggatcgaggc cgacaagccg catcagcgtc
     2161 tccgccctcc catgtgcaac agtgcgcgac aggcccccgc gttcacgcag cacctcgcgc
     2221 atctgggcgc cgacgcgctg caccggatcg agcgcgttca tcgcagtctg cggtacgaac
     2281 gacagacgcc gccaccgcag cgcacgcaac tcgcgctctg atagcctcgc gagatccttg
     2341 ccctcgaatg tgatactgcc accggcaatg cggccagacc ccggcagcac gcccatcagc
     2401 gcgcggccta gtgtcgactt gccgcagccg ctttcgccaa ccagaccgac gatgctgccg
     2461 gcgggcacgt ccaggtcaac gccatcaacc gcacggacag tgccaccacc agtggcgtag
     2521 tgcacatgta gatctcgaat cgcgagcaac gtcattgaac cacagatgaa cacagataca
     2581 cacagataga caaaatctgt gtccatctgt gtgtatctgt ggttccatat tcttctgtca
     2641 tgcgtcagcc agcttcggga acagcagctt ctctgacccg cgcgagatca gaaaccccgc
     2701 cagcaccatc aacacgatcg ccgcgccc
//
LOCUS       DATGEM010000455         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_808274, whole genome shotgun sequence.
ACCESSION   DATGEM010000455 DATGEM010000000
VERSION     DATGEM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808274"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VKQ27_14420"
     CDS             <1..1052
                     /locus_tag="VKQ27_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HLY90173.1"
                     /translation="APMTATFFFPAMAGIVAAHSRRSKVAPMIPPKDQIKVCFAHAAY
                     QMKASFDALNTGIASFELRDGETLDRRIGEADVLVVSGMWHNGLIELGGRLRFIQSIG
                     SGTDQFDKEKLTAKGVRLASAAGVNARAVAEHAMSLILALSRRLPEARDNQAKRVWRG
                     MIGDLTQREDELQGKTALIIGLGRIGGRLAQLAKAFEMQVIGFRRHPASGAGAADAVH
                     PLTELPAFLPRADFVVLTCPLVPETRGVIDAKALGLMPASAFLVNCARGGCVVEPALV
                     EALRAGQIAGAALDVTDPEPAQEASPLWSMPNVFITPHTGGETRKYEVNVNEILVENL
                     ERMWRGETELRNQVV"
     gene            1190..2620
                     /locus_tag="VKQ27_14425"
     CDS             1190..2620
                     /locus_tag="VKQ27_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLY90174.1"
                     /translation="MIRQPEPKADVLARRDEIVAGLRALLPPDGVIAEPLLLRPYETD
                     GLSAYRQPPLAVVLPETTEQVAAVMQHCHANGVRVVPRGAGTSLSGGALPMADAVVVG
                     LMRMNRILDIDYADRCATVQAGVTNIGITNAVQAKGFFYAPDPSSQLACMIGGNVNMN
                     SGGAHCLKYGVTANNLLGLRIVTVEGEILDIGGAHLDAAGYDWLGLLTGSEGQLAVVT
                     EVTVRILHSPEGARAMLAAFRSNEVAGACVDSIISSGIIPVALEFMDRPAIHACEAFA
                     HAGYPLDAEAMLIIEVEGSAAEQDDLLARLRAICQKHDPISLKVSQSAEESLAIWKGR
                     KGAFGAIGRISPDYLCMDGTIPTSRLPDVLRRIGEMSDAYGLKVANIFHAGDGNLHPL
                     IMFDANDPESFHKAEQFGADILKLCVEVGGCLTGEHGVGVEKRELMPVQFTAQELEQQ
                     RRIKAAFDPGWLLNANKVFPLAAASA"
     gene            2654..>3596
                     /locus_tag="VKQ27_14430"
     CDS             2654..>3596
                     /locus_tag="VKQ27_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLY90175.1"
                     /translation="MSTVLAPSDEAGFAAVIAEAAAAREPLAIIGAGTKAGMLRPVQA
                     ARSLSTRGHAGITLYSPHELIISARAGTPLAEIEATLAEQGQHIIAEPPDLGPLFSTQ
                     ERQTIGGVIATNLSGPRRVAWGAMRDHLLGIRAVTGRSEVVRSGGRVLKNVTGLDLCK
                     LLAGSHGTLGVMTEITLKVLPAPEAVGTLVLHNLDATAGVAQLSAALGSPYGISGAAW
                     LPADCAARLGAFAGSVALIRIEDFAPSVAYRTARLRDDIGHAEADILGDSASRAVWRA
                     LRSMAALPVGHDDAVWRVSVRPSAGPAVLQALADGVGM"
BASE COUNT          582 a         1099 c         1274 g          641 t
ORIGIN      
        1 cggcgccgat gaccgcgacc tttttcttcc ccgccatggc cggtattgtc gcggctcatt
       61 cccgccgcag caaggttgcc cccatgatcc cgcccaagga ccagatcaag gtctgtttcg
      121 cccatgcagc gtaccagatg aaggcgagct tcgacgcgct gaacaccggc atcgccagtt
      181 tcgagctgcg cgatggggag acgctggacc ggaggattgg tgaggccgac gtgctggtgg
      241 tctcgggcat gtggcacaac ggactgatcg agctgggtgg gcggctgcgc ttcatccagt
      301 cgatcggttc ggggaccgat cagttcgaca aggaaaagct gaccgcgaag ggtgtgcggc
      361 tggccagcgc ggccggcgtg aatgcacgcg cggtggcgga acacgcgatg tcgctgatcc
      421 tggcgctgtc gcgacggctg ccggaggcgc gcgacaatca ggcgaagcgt gtgtggcgcg
      481 gcatgatcgg tgacctgacg cagcgcgaag atgagctgca ggggaagacc gcgctgatta
      541 tcggtctggg ccgcatcggc gggcggctcg cacaactcgc caaggcgttc gagatgcagg
      601 tgatcgggtt tcggcgccat ccggcgtctg gcgcgggtgc cgcggatgcg gtgcatccgc
      661 tgacggaatt gccggcgttt ctgccacggg cggatttcgt ggtgctgacg tgtccgctgg
      721 tgccggagac gcgcggggtg atcgatgcga aggcgctggg gctgatgccg gcgtcggcat
      781 tcctggtgaa ctgcgcgcgg ggtgggtgcg tggttgagcc ggcgttggtg gaggcgttgc
      841 gcgcggggca gatagcgggt gctgcgctgg atgtgaccga ccccgaaccg gcgcaggagg
      901 cgtcaccgct gtggagcatg ccgaatgtgt tcatcacccc gcacacgggc ggcgagacgc
      961 gcaagtatga agtcaacgtc aacgagatcc tggtggagaa cctggagcgg atgtggcggg
     1021 gcgagacgga gcttcggaac caggtcgtct gatgagggtt gcgggctgcc acgtccttac
     1081 ttgaacaacc tggatcggga ttgccgcgtc gcttcgctcc tcgcaatgac aggggccgtc
     1141 tgatgcggat cgcttgcctc tgttgggttg gcggcccatc ttccggagca tgatcagaca
     1201 accggaaccc aaggccgacg tcctggcgcg acgcgacgaa atcgtggccg ggctgcgggc
     1261 gctgctgcct ccagatgggg tgatcgccga accgttgctg ttgcggccct acgagaccga
     1321 cggattgtcg gcgtatcgcc agccgccgct cgccgtcgtg ctgccggaga cgacggagca
     1381 ggtcgccgcg gtgatgcagc attgccacgc gaatggcgtt cgcgtggtgc cgcgtggtgc
     1441 cgggacatcg ctgtcgggcg gcgcgctgcc gatggcggat gcggtcgtgg tcgggctgat
     1501 gcggatgaac cggatcctcg acattgatta cgccgaccgc tgcgcaacgg tgcaggcggg
     1561 cgtgaccaat atcggcatca ccaatgcggt gcaggcgaag gggttcttct atgcccccga
     1621 tccgtccagc cagctcgcct gcatgatcgg cggcaacgtc aacatgaatt ccggcggcgc
     1681 tcactgcctg aaatacggcg tcaccgccaa caatctgctc ggcctgagga tcgtcacggt
     1741 ggagggtgaa atcctcgaca tcggcggcgc gcacctcgat gccgcaggtt acgactggct
     1801 tggcctgctg accggcagcg aaggccagct cgcggtggtc accgaggtta cggtgcgcat
     1861 cctgcattct cccgagggcg cacgggcgat gctggcggcg ttccggtcga atgaagtcgc
     1921 tggcgcgtgc gtggactcga tcatttcgtc cggcatcatc ccggtggcgc tggagttcat
     1981 ggatcgcccc gccatccacg catgtgaggc cttcgcccac gccggctatc cgctcgatgc
     2041 cgaggcgatg ctgatcatcg aggtggaagg cagtgcggcg gaacaggacg atctgctggc
     2101 gcggctgcgc gcgatctgcc agaagcacga tccgatcagc ctcaaagtat cgcagtcggc
     2161 ggaggaatcg ctggcgatct ggaagggccg caaaggtgcg ttcggcgcaa ttggccggat
     2221 cagcccagac tacctttgta tggacggcac catccccaca tcgcgcctgc ccgatgtgct
     2281 gcggcggatc ggtgaaatgt cggacgccta cgggttaaag gtcgcgaaca tattccatgc
     2341 cggcgacggc aacctgcatc cgctgatcat gttcgacgcc aacgatccgg agagcttcca
     2401 taaagcggaa caatttggtg ccgacatcct gaagctctgc gtggaggtgg gtggttgcct
     2461 caccggcgag catggtgtcg gcgtggagaa gcgcgagctg atgccggttc agttcaccgc
     2521 tcaggagctc gaacagcagc gacgcatcaa agccgcattc gatcccggtt ggctgctgaa
     2581 tgccaacaag gtgttcccac tggctgctgc gtcggcctga ggccggcgct ggctcgggcc
     2641 tcgcaacacc gccatgagca ccgtgctcgc accatccgat gaggctgggt tcgccgctgt
     2701 gattgcagaa gctgccgcgg cgagggagcc tctggccatc atcggtgccg gtaccaaggc
     2761 ggggatgctg cgcccggtcc aggcggcgcg ctcgctgtcc acgcgtggcc atgccggcat
     2821 cacgctgtat tcgccgcatg agctgatcat ctccgcgcgc gccggcacgc cgttagcgga
     2881 gatcgaggcc acgctggccg agcagggaca gcacatcatc gccgaaccac ccgaccttgg
     2941 cccgctgttc agtacacagg agcggcagac catcgggggc gtgattgcga caaacctgtc
     3001 gggtccgcgc cgtgtcgcct ggggggcgat gcgcgaccat ctgctgggta tccgcgctgt
     3061 cactggacgc agcgaggtgg tccgctccgg tggccgcgtc ctcaagaacg tgactggact
     3121 ggacctgtgc aagctgctcg ccggcagcca tggcacgctg ggcgtcatga ccgagatcac
     3181 actgaaggtg ctgcctgcgc cggaagccgt cggcacgctg gtgctgcaca atctcgacgc
     3241 gacggctggt gttgcgcaac tgtcagccgc actgggttca ccctacggca tatcgggggc
     3301 ggcctggctt cccgccgatt gcgccgcgcg gctcggcgca ttcgccgggt ctgtcgcgct
     3361 gatccgtatc gaagactttg caccgtcggt cgcttaccgg actgcacggc tgcgcgacga
     3421 tattgggcac gcggaagcgg acatcctcgg tgactcggcg tctcgcgcgg tgtggcgggc
     3481 gctgcgctcg atggcagcac tgcccgttgg tcacgacgat gcggtatggc gcgtatcggt
     3541 acgtccctcc gctggcccgg cggtgctgca agccctcgcg gatggcgtcg gcatgc
//
LOCUS       DATGEM010000456         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1224876, whole genome shotgun sequence.
ACCESSION   DATGEM010000456 DATGEM010000000
VERSION     DATGEM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224876"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VKQ27_14435"
     CDS             <1..678
                     /locus_tag="VKQ27_14435"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLY90176.1"
                     /translation="QSGNDRTGILDASSLREALARFQDPLPHVARWQIVTTILPLIVL
                     LVAMYAGLAFGWWPVLALGVPAAVLTVRTFIIQHDCGHGSFCRARHANDLLGRFCSLL
                     TLTPYAQWRRHHAKHHGAWNDLDRRSTHGRDIYSSCLTVAEYNVLSTWRRLLHRFTKH
                     PAISLLVLPPLIFWCCIASRSPRRAPGRAERRSVYLTNLSLVALHGTLAAMLARRIHE
                     VAARRLA"
     gene            complement(784..2083)
                     /locus_tag="VKQ27_14440"
                     /pseudo
     CDS             complement(784..2083)
                     /locus_tag="VKQ27_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT          414 a          732 c          715 g          445 t
ORIGIN      
        1 caatcgggca acgatcggac aggaatactt gatgcttcca gcctgcgcga agccctggcg
       61 cgcttccagg acccattgcc gcacgtcgcc aggtggcaga tcgtcaccac aatcctgcct
      121 ctgatagttc tgctggtggc gatgtatgca gggctggcgt tcggctggtg gcctgtgctc
      181 gcgctaggcg ttccggcggc tgtgctgacg gtgcggactt ttatcattca acacgattgc
      241 ggccatggat ccttctgccg cgcgcgccac gccaacgacc ttctcgggcg attctgctcg
      301 ctgctaacgc tgacgcctta cgcccagtgg cggcgccacc acgcgaaaca ccacggcgcc
      361 tggaatgacc tcgaccggcg cagcacccac ggacgcgata tatattcgtc ctgcctcacg
      421 gtcgccgagt ataatgtcct cagcacctgg cgtcggctac tccaccgttt taccaagcat
      481 cccgcaatca gcctgctggt gctaccgccg ctaatttttt ggtgttgtat cgcttcccgt
      541 tcgccgcgcc gcgcacctgg gcgcgcggaa cggcgcagcg tgtaccttac caacctctcg
      601 ctggttgcgc tccatggcac tctcgcggcg atgctagccc gacgtattca cgaggtggcg
      661 gctcgcagac tggcatgagc ggcggcaagc ttgttggtgg cgcgtttgcg cgcgatgtcc
      721 gctcggtttg ttcggcgctg gaggttacga cgaccgggct gttcggggca cgcgccccgg
      781 tgattatggt cctgcgggtt gcaggctgcg agtgatgctg cggaacagtc cagcctcggg
      841 gcaagcggcg gcgaaggcaa tgcgcacgcg tgtagcgctc tctgtgatcc gtgctgcgac
      901 cttgaggagg cgcatccgca aggtcgtgaa ctcggcggtg gcgagcggat gcagcttcgc
      961 aatagcgtcg cgcatcgtca gcatcagcca gtaggcagcg gtgtgcagta taagacgcac
     1021 ctgattggcg agtgccgaac gacaactggt gcgatcggag gcaagctggc tcttgtgaag
     1081 cttgatcaga ttttcggctt ggccacgcgc gcagtagacg gtgtcgtata gccattccgc
     1141 gctaccgcct tccaggttgg tcaccacgta gcggatatcc aacccctgca ttgtggcctc
     1201 aatccgtgcc gccacgcggc gctcgcagcc ccaggatttg gcgccatagc gggtctcagt
     1261 atagcggcga accaccgccg cctgggcttc ggcgcgacgc actctgacat cgtcggcagc
     1321 gacctcgatc agacggccca gcgtggcgtt gcccggcagg ccgagcacgt agtccacatc
     1381 gttgatctcg caccacgcca tgatctcggg acggccatag tgaccatcgc cacgaatggt
     1441 cagccgcgtg ctcggccagt ggctgcggat gcggcgcacc agacgacgca gatgacgacg
     1501 gacttcgtct cccgaaggtg tcttgccggg ccgcaacagc accgccaccg gacgggacgt
     1561 cgcagtctcg tagacgtgga tcggcaggaa gcagcgttca tcgtaatacg cgttgaacaa
     1621 tgaaagctgc tgatggccgt gcacgacatc gaccgtgtcg tcgatgtcca gcgtgacggc
     1681 ggcgggcggg tgagaatagc tggcacaata ggcgtcgatc atcgcgtagg tcatgcgtac
     1741 cacctcacgc aagctcggcg cattttcccg gcgcgacatc gtcggctgcg agcacagatc
     1801 atcgccgcta tcgggcagac gcccgcaagc cagcctgaag ccgggatcgc tgcgcagata
     1861 atccagatca tcggcatcct catatccgca ggcgatggcc aacatgcgcg cccgcagaat
     1921 gtcgatgaca ctgtgcgtca ccagcagggg attgcggcga tcggcgatca acccggccaa
     1981 ccgctgtgcg atcccaatac gccgctccgc catcaaacgc ggcagtgact ttcttgcggc
     2041 taactgctgg aaagctgaac ggctcgatcg tatcgtcatg catggcgggt gtggctcagc
     2101 ttgatccggt gaatggactg gtctcgacac ccaatcccta aacaggatca gtcgcttacg
     2161 cgacacccgc cagcgaatcc cagccatacg gtgaataaga gcggcttctg taacgggcca
     2221 ccgtgtgtca atcccattcg agcttgatcc tgccgccggt gtcttgcttc tgtccggggt
     2281 cagcgcgcag ctgccacctg atgggg
//
LOCUS       DATGEM010000457         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_929039, whole genome shotgun sequence.
ACCESSION   DATGEM010000457 DATGEM010000000
VERSION     DATGEM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929039"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1908)
                     /gene="aspS"
                     /locus_tag="VKQ27_14445"
     CDS             complement(121..1908)
                     /gene="aspS"
                     /locus_tag="VKQ27_14445"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLY90177.1"
                     /translation="MHAYRTHTCGALRASDAGHPARLSGWVHAKRDHGGLLFIDLRDH
                     YGITQCVFAAGSPAFAAADAVRPESVITVTGEAVNREAGTVNPRLPTGEIELRVSDLT
                     VQSAAEVLPMQVAGDEKFPEDIRLRYRFLDLRREKLHRNIMLRAGVIASIRRRMIDAG
                     FTEFQTPILTADSPEGARAFLVPARLHPGKFYALPQAPQQFKQLAMVAGFDRYFQIAP
                     CFRDEASRADRSPGEFYQLDFEMSYVTQEDVFVTIEPVIAGVFEQFAEGRAVTPSPWP
                     RIPYDTAMLEYGSDKPDLRNPLKVADVTQHFAGSGFGLFARIAASGGVVRAIPAPGAA
                     SQPRSFFDKLNDWAKAEGAGGLGYIVYDADGPKGPIARNLEADRAEAVRAACGVKAGD
                     AVFFAAGKREDAARFAGVVRTRLGEELDLIEKGAFRFCWITDFPMFELNEETKQVDFS
                     HNPFSMPQGGLEALNSQDPLTIKAFQYDIVCNGAELCSGAIRNHRPDIMVRAFEIAGH
                     PPEEVEKRFGGMLNAFRYGAPPHGGAAPGIDRIVMLLADEPNIREVILFPLNQQGEDL
                     MMGAPAEVPPNRLRELHLRLDLPPKVRAS"
     gene            complement(1968..3122)
                     /locus_tag="VKQ27_14450"
     CDS             complement(1968..3122)
                     /locus_tag="VKQ27_14450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLY90178.1"
                     /translation="MSGMDIVRIPGSRILDIAKIAFATPDVDFLCFGESDQPSPDAAH
                     VAAVASLAAGETRYSDVRGLPALRIALSDYLTALHARPVPEDRIQVTASGMAAVATAL
                     AATVRAGDRVVLHSPAWPNVGNAARLRGAEVVELPLTPLAEGGFRMDFDRLDTLLDGA
                     RAFIVNSPNNPTGWTATLDELATILEIARRRGVWLIGDEVYSRLIYDGAEAAPSLLDL
                     AEPDDRVIVCNSFSKTWVMTGWRLGWLVVPDGTRDAITELVEVAHSGVAPFIQQAGVA
                     AVKDAATVERFRAHCATGRKLTSEALGGLNGVRYVEPIGAFYAFVGIEGVTDSLGLAR
                     RLVMEHGVAVAPGIAFGDAGEGYLRICFAQSAERIERAMGRLREGLRARA"
     gene            complement(3144..3623)
                     /locus_tag="VKQ27_14455"
     CDS             complement(3144..3623)
                     /locus_tag="VKQ27_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY90179.1"
                     /translation="MNRGDKPSGFDPFSLPESNATTYPEAFRADNENRWNRRLGNHAG
                     LTNFGVNLTRIVPGGQSSHRHAHLRQDEFIYVLQGTLELETNAGTEVLHAGMCAGFPA
                     GSGNAHRFVNRSAEDVLLLVIGDRTPSEEVTYPDIDLELHQEGDGPFRYFHKDGTPY"
     gene            complement(3620..>4117)
                     /locus_tag="VKQ27_14460"
     CDS             complement(3620..>4117)
                     /locus_tag="VKQ27_14460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90180.1"
                     /translation="IHAFGYGGSALTSGGWPSYYLEEMVGHAQCQQAVLTSLIFEGVF
                     ERFPTLKVVLIEAGFAWAASHSWRMDRQFAKLRKEVPHVKRLPSEYIRSNVWFTTQPV
                     EEPEPREHLADAIEWMGWDRVLFATDYPHWDFDDPSYALPLKMTEAQKRGIFLENARA
                     VYGFA"
BASE COUNT          766 a         1404 c         1258 g          689 t
ORIGIN      
        1 attgaggaac tccgctgggg gcaggcgcca acatagggac gcagcagcta taccgccagc
       61 gatcccgcca tattcgggcc atgcctgtca tccccagatg acggaagcgc caggccggcg
      121 ctagctggca cgcactttcg gcggcagatc gagccgtagg tgcaactccc tcagtcgatt
      181 cggcggcacc tcggcaggcg cccccatcat cagatcctcg ccctgctgat tgagcgggaa
      241 aaggatcact tcgcggatgt tcggctcatc cgccagcagc atgacaatgc gatcgatgcc
      301 aggcgccgcc ccgccgtggg gtggcgcgcc gtaacggaac gcgttcagca tgcctccgaa
      361 gcgcttctcg acttcctcgg gtggatggcc ggcaatttcg aacgcgcgga ccattatgtc
      421 gggtcgatgg ttccggatcg cgcctgagca caattcggcg ccattacaga cgatgtcgta
      481 ctggaacgcc ttgatcgtca ggggatcctg cgagttcagg gcctccaacc cgccctgtgg
      541 catgctgaac gggttgtgac tgaagtcaac ctgtttggtc tcctcgttca actcgaacat
      601 cgggaaatcg gtgatccagc agaagcggaa tgcgcccttc tcgatcagat ccagttcctc
      661 gccgagccgc gtgcgtacca cgccagcgaa cctggccgcg tcctcccgct tgccggccgc
      721 gaagaacacc gcatcgccgg ccttcacccc gcacgccgca cgcaccgctt cagcgcgatc
      781 ggcttccagg ttgcgggcga ttggaccttt cggcccatcc gcatcgtaca cgatatagcc
      841 gagccccccg gcaccctccg ccttcgccca gtcgttcagc ttgtcaaaga aactgcgcgg
      901 ctgcgatgct gcacccggtg cggggattgc ccgcaccaca ccccccgatg ccgcgatccg
      961 cgcgaacagc ccgaagcccg atccggcgaa atgctgcgtc acgtccgcga ccttcagtgg
     1021 attgcgcaga tccggcttat cggagccgta ttccagcatc gcggtgtcgt aggggatgcg
     1081 cggccacggc gacggcgtga cagcccgccc ttcggcgaac tgctcgaaca cgccggcaat
     1141 gaccggctcg atcgtgacga acacgtcctc ctgcgtgacg tagctcattt cgaaatcgag
     1201 ctggtagaac tcccccggcg accgatcggc acgcgaggct tcatcgcgga aacacggcgc
     1261 gatctggaag tagcgatcga agcccgccac catcgccagc tgcttgaact gctgcggtgc
     1321 ctgcgggagt gcatagaatt tgcccggatg cagccgcgcc ggcaccagaa aagcgcgcgc
     1381 tccttcgggc gagtcagcgg tcaggatagg cgtctgaaac tcggtgaatc cggcgtcgat
     1441 cattcgccgg cgaattgagg cgatcacacc ggcgcgcaac atgatgttgc gatgcagttt
     1501 ctcgcggcgc aggtcgagga aacggtaccg cagccggatg tcctccggga acttctcgtc
     1561 gccggcgacc tgcatcggca ggacctcggc tgccgactgc acagtcaggt ccgacacgcg
     1621 cagttcgatc tcacccgtgg gcagccgagg gttcactgtc ccggcctcgc ggttcaccgc
     1681 ttcgccggtg acagtgatta cgctctccgg acgcaccgca tcagcggcgg caaaggcggg
     1741 cgagcccgcg gcaaacacgc attgtgtgat gccgtaatgg tcgcgcagat cgatgaacag
     1801 cagtccacca tgatcccgct tggcatgcac ccagccggaa aggcgggcgg gatgcccggc
     1861 atcgctggcg cgcagggcgc cgcaggtgtg ggtgcggtaa gcgtgcatgt ggatttgtcc
     1921 cgctgatcga gggcggcaaa caaccggcgg ccaccccggc tgtcaagtta ggcccgcgcg
     1981 cgcaatccct cacgcagccg ccccatcgcc cgttcgatcc gttccgccga ctgcgcaaag
     2041 cagatgcgca gatagccctc gccggcatct ccgaacgcaa tcccgggcgc cactgcaacg
     2101 ccatgctcca tcaccagccg ccgcgccagg ccgaggctgt cagttacccc ttcgatcccg
     2161 acgaacgcat agaacgcacc aatcggctcg acgtaacgca cgccgttgag gccaccgaga
     2221 gcctcgctag tcagcttgcg gccggtggca caatgcgcac gaaagcgctc cacggtcgcc
     2281 gcatccttca ctgccgcgac tcccgcctgc tggatgaacg gcgcgacgcc cgaatgggcg
     2341 acctcaacca gttcagtgat cgcgtcgcgc gtaccgtccg gcaccaccag ccagcccagc
     2401 cgccatccgg tcatcaccca ggtcttagag aaactgttgc acacgatcac ccgatcgtcc
     2461 ggctcggcca gatcgagcag cgatggcgca gcctccgccc catcatagat caaccgcgaa
     2521 tagacctcgt cgccgatcag ccatacgccg cggcggcgcg cgatctcgag gatggttgca
     2581 agctcatcga gggtcgcggt ccagccggtg gggttgttcg ggctgttgac gatgaacgcg
     2641 cgtgcgccat ccaacagcgt gtcgagccga tcgaagtcca tgcggaagcc gccttcggcc
     2701 aacggcgtga gcggcagttc gacgacctcg gcaccacgca gccgcgccgc gttgccgaca
     2761 ttcggccagg ccggactatg cagcacgacc cgatcaccgg cgcgcaccgt tgccgcgagc
     2821 gcggtcgcaa ccgcggccat gccggaggca gtcacctgga tgcggtcctc gggcaccggc
     2881 ctggcatgca gcgcggtgag atagtccgac agggcgatgc gcaaggcggg aaggccgcgc
     2941 acatccgaat atcgggtctc acccgcggcc agcgacgcga cggcagcgac gtgtgccgcg
     3001 tcggggcttg gctggtcaga ttcgccaaaa cagaggaagt cgacatccgg cgtggcgaag
     3061 gcaatctttg cgatgtccag gatgcgtgat cccgggatgc gaacgatgtc cattccactc
     3121 atattggtcg cgccaagcgg ctttcagtac ggcgtgccat ccttgtggaa atagcggaac
     3181 ggaccatcgc cttcctggtg cagctccaga tcgatgtccg gatacgtcac ctcttcgctg
     3241 ggcgtacgat cgccgatgac cagcagcaac acatcctcgg ccgaccggtt cacgaaccga
     3301 tgcgcgttgc cgctgcctgc cggaaagccg gcgcacatgc ctgcgtgcag gacctcggtg
     3361 ccggcattgg tctccagctc caaagtacct tgcagcacat agatgaactc gtcctgccgc
     3421 agatgcgcat gccggtgcga tgactgaccg cccggtacga tccgcgtcag gttcacgccg
     3481 aaattggtca gcccggcatg attgccgagc cgacggttcc agcggttctc gttgtcggcg
     3541 cggaacgcct cgggatacgt cgtcgcattg ctctctggca atgagaacgg atcgaagccc
     3601 gatggcttgt cgccgcggtt cacgcgaagc cgtacaccgc cctggcgttc tccaggaaga
     3661 tgccgcgctt ctgcgcctcg gtcatcttca gcggcaacgc ataggacgga tcgtcgaagt
     3721 cccagtgcgg atagtcggtc gcgaacagca cgcgatccca acccatccat tcgatcgcat
     3781 cggcgagatg ctcgcgcggc tccggctctt ccacgggctg cgtggtgaac cagacattgc
     3841 tgcggatata ttcggagggc agccgcttca cgtgcggcac ctccttgcgc agcttcgcaa
     3901 actgccgatc catccgccag gaatgcgacg ccgcccaggc gaagccggcc tcgatcagca
     3961 cgaccttcag cgtcggaaag cgctcgaaca cgccctcgaa gatcaggctg gtcagcacgg
     4021 cctgctggca ctgtgcatgc ccgaccatct cttccagata gtaagatggc cagccacccg
     4081 aggtcagtgc gctgccgcca tagccgaacg catggat
//
LOCUS       DATGEM010000458         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_665364, whole genome shotgun sequence.
ACCESSION   DATGEM010000458 DATGEM010000000
VERSION     DATGEM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665364"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VKQ27_14465"
     CDS             <1..424
                     /locus_tag="VKQ27_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90181.1"
                     /translation="DEWSKIGVKVTQKVVPTGPWFDAMRTGNFDVVVEANCNGVVNPV
                     MDTQKYLPKKAFVENYGGYEDQELIDIYGKMLHETDPAKQRVFMRQYETRVVDTMAYE
                     WPMLWWNRMLPERSYMRGWKIGPSHYVNQDLANIWLDK"
     gene            complement(515..1243)
                     /locus_tag="VKQ27_14470"
     CDS             complement(515..1243)
                     /locus_tag="VKQ27_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY90182.1"
                     /translation="MSAERPILIVDDDSVLRETLADQLAHDGEFAAVGAGSINEAEGL
                     LSSKNARFDALILDVSLPDGDGRDLCNRLRKQGQKMPIIMLTGSDAETDVVRGLDSGA
                     NDYIAKPFRLNELLARLRAQLRIFENSEDAVFTIGPYTFRPSAKLLQDPGKNRRIRLT
                     EKEAAILKFLYRAGTRAVARQVLLNEVWGYNAAVTTHTLETHIYRLRQKIEPDPTNAR
                     LLVTEGGGYRLDPEGVPASSPVAA"
     gene            1621..2727
                     /gene="ribA"
                     /locus_tag="VKQ27_14475"
     CDS             1621..2727
                     /gene="ribA"
                     /locus_tag="VKQ27_14475"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HLY90183.1"
                     /translation="MTVPDPAILGLRIVHRAASDLRQGTPVLLTGSAALVLLAAETAS
                     ARGLGELMTLAAEPPVLLLAPVRAAAVLHQPIEAGASVMALRLDEALLAPGPLHGMAD
                     PTVQEVLPVQPGAALVPELALTALSLAKLGRLLPAVLAAPVRPEVLEDVARLGLIAVP
                     ADDVASYPVAAAAELKQVAAAPVPLEGAEDSRVIVFRTEGSAIEHLAIVMGHPETVET
                     PLVRIHSECFTGDLLGSLRCDCGAQLRGAIARIADEGAGVLLYLAQEGRNIGLVNKLR
                     AYTLQDRGLDTLDANRALGWGADERNFLIAATMLHAIGVHRIRLMTNNPDKIAALAAC
                     GVDVVGREAHQFAPNGVNDHYLATKAERFGHLLD"
     gene            2727..3218
                     /locus_tag="VKQ27_14480"
     CDS             2727..3218
                     /locus_tag="VKQ27_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLY90184.1"
                     /translation="MQVIVHPDGRLVLRGTVFRAALGRGGVRRDKQEGDGATPVGVLP
                     LRRVLYRADRGRAPECAVPVEPIAPTDGWCDGPNERDYNRIIRLPYDGHYEELWRTDA
                     AYDVIGVLGWNDAPIQRGRGSAIFLHVARPDYAPTDGCIALAQNDLRQVLAAGTTEVV
                     VSG"
     gene            complement(3215..3574)
                     /locus_tag="VKQ27_14485"
     CDS             complement(3215..3574)
                     /locus_tag="VKQ27_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HLY90185.1"
                     /translation="MPAVPTEIRLNRAERVLHVAFDDGSAFALPVEYLRVESPSAEVQ
                     GHGPDQKKIVAGKRNVAVTSIEPVGNYAVRLVFDDRHDTGIYSWDYLLELGREHDRRW
                     SDYLAALELRGMNRDRA"
     gene            complement(3574..3777)
                     /locus_tag="VKQ27_14490"
     CDS             complement(3574..3777)
                     /locus_tag="VKQ27_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HLY90186.1"
                     /translation="MDEPTETRPQPPLDPRLLEILVCPLTKGPLEYDRDAGELISRKA
                     GLAYPVRDGIPIMLPEEARKLEE"
     gene            complement(3792..>4115)
                     /locus_tag="VKQ27_14495"
     CDS             complement(3792..>4115)
                     /locus_tag="VKQ27_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HLY90187.1"
                     /translation="RVAGDFSAYHGDLDLTAQPVGVDREALLTALRGYFTLRGFDANW
                     DAIKRLDDDMLVITLAMVCPFEPAEKQALLEAPTQAERGAALLVLLQMGALAPDTSSG
                     GRSVS"
BASE COUNT          685 a         1249 c         1402 g          779 t
ORIGIN      
        1 cgatgagtgg agcaagatcg gcgtgaaggt cactcagaag gtcgtgccga cgggcccatg
       61 gttcgacgcg atgcgcaccg gcaatttcga cgtcgtggtc gaagccaatt gcaacggcgt
      121 ggtcaacccg gtgatggaca cgcagaaata cctgccgaag aaggcgtttg tggagaacta
      181 cggcggctac gaggaccagg agctgatcga tatctacggg aagatgctgc atgagacgga
      241 tccggcgaag cagcgtgtgt tcatgcggca gtatgagaca cgggtggtcg acacgatggc
      301 ctatgagtgg ccgatgcttt ggtggaatcg gatgctgccg gaacgttcct acatgcgcgg
      361 ctggaagatc ggcccgagcc attacgtcaa ccaggatctc gccaacatct ggctggataa
      421 gtagtgggcc ttactccgcc aagcgcgtcc agggcgcatg ccgatgtggc acgcgccctg
      481 gttggcggga tgcgatgagt ggaggaagct ctcgctaggc tgccactggc gaggaggccg
      541 gcacgccctc ggggtccagg cgatagccac caccttccgt caccagcagg cgggcattgg
      601 tcgggtccgg ctcgatcttc tggcgcaggc ggtagatgtg cgtctccagc gtgtgcgtgg
      661 tgacggcggc gttgtaaccc catacctcgt tcagcagcac ctggcgggcg acggcgcgcg
      721 tgccggcgcg atacaggaac ttgaggatcg ccgcttcctt ttcggttagc cggatgcggc
      781 ggtttttgcc ggggtcctgc agcagcttgg ccgacggccg gaacgtgtag gggccgatgg
      841 tgaacaccgc atcctcgctg ttctcgaaga tgcgaagctg cgcgcgcagc cgtgccagca
      901 actcgttcag gcggaacggt tttgcgatgt agtcgttggc gcccgagtcg aggccccgca
      961 ccacatcggt ctcggcatct gaaccggtga gcattatgat cggcatcttc tgcccctgct
     1021 tgcgcaggcg gttgcagagg tcccggccgt cgccatcggg caggctgacg tcgaggatca
     1081 gcgcatcgaa ccgagcgttc ttagagctca gcaggccttc ggcttcgttg atggagcctg
     1141 cgccgacggc ggcgaactca ccatcatgtg ccaactgatc ggccaaggtc tcgcgcaata
     1201 cgctgtcatc gtcgacaatc aggatcggtc gttccgccga cataatcact tcctctcgct
     1261 ctgtcgggcg cactgcgccc gttgctatcg cgcgccttta gaggcccggc tgtggcctgc
     1321 gccacaacga gcggcctccc cgacattgcg gccaacaccc aaaggtcatc ctgccactcc
     1381 ggttcaggcc gtcgcaaggt cactcacatt tctgtgagcc atgccgagac attttgtccg
     1441 tcccgccata tagttgggaa cgcggtttca tgcatcaggc agtgtgagtt ccgcctgtcg
     1501 ctcaggaagg caggttaacg tgaatcttga ccaaattggg ccgttactgt tccattttcg
     1561 tgtacgacgg tgaaacaggt gcttcgcgct gaacaagtta tcgtggcgga tacgcatgct
     1621 gtgaccgttc ctgatccggc gatccttggc cttcggatcg tgcatcgcgc cgcttccgat
     1681 cttcggcagg gcacgcccgt attgctgacg ggaagcgcgg cgctggtgct gctcgcggct
     1741 gagacagcga gcgcgcgggg cctgggcgag ctaatgacgc ttgctgccga acccccggtg
     1801 ctgctgctcg cgccggtacg ggcggcggcg gtgctgcacc aacctattga ggcaggggca
     1861 tcggtgatgg cgctgcggct ggatgaggcg ctgctggcgc ctgggcctct gcacggcatg
     1921 gcggacccga cggtgcagga ggtgctgccg gtccagccgg gggcagcact ggtgccggag
     1981 cttgcattga ctgcgctgag cctggcgaag ctcggtcgtc tgctgcctgc ggtgctggcg
     2041 gcgccggtca ggccggaggt attggaggat gttgcgcgcc ttgggctgat cgccgtgcca
     2101 gccgatgatg tcgcgagcta cccggttgca gcagcggcgg aactgaagca ggttgccgca
     2161 gcgccggtgc cgctggaggg agccgaggac tcgcgggtga tcgtgttccg taccgaaggg
     2221 tcggcgatcg agcacctcgc catcgtcatg ggccaccccg agacggttga gacgccgctg
     2281 gttcgcatcc actctgagtg tttcaccggt gacctgctgg gcagcctacg ctgcgactgc
     2341 ggtgcgcagc ttcgcggcgc gatcgcgcgt attgccgacg aaggggcagg cgtcctgctc
     2401 tatctggcgc aggaagggag aaacatcgga ctggtcaata agttgcgagc ctacacactg
     2461 caggaccgcg gcctggatac tttggacgcg aatcgggccc tgggctgggg agcggacgag
     2521 cggaacttcc tgattgctgc cactatgctc cacgcgatcg gggtgcatcg catccggctg
     2581 atgaccaaca accccgacaa gattgccgca ctcgctgcat gcggtgtgga cgttgtcggc
     2641 cgtgaggcac atcaattcgc gccgaacggc gtcaacgacc actatctggc gaccaaggcg
     2701 gagcgcttcg gtcacttgct cgactgatgc aggtgatcgt ccatccggat ggccgcctcg
     2761 tgctgcgagg gacggtgttc cgcgccgcgc ttggacgcgg gggtgtgcgg cgcgacaagc
     2821 aggaaggcga cggggccacg cctgtcggcg tgttgccgtt gcggcgggtg ctgtaccggg
     2881 ctgatcgcgg ccgggcgccg gaatgcgctg tacctgtgga gccgatagcg cctacggatg
     2941 gctggtgcga cggtccgaac gaacgcgact acaaccgtat tatccgattg ccctatgacg
     3001 gtcactacga agagctgtgg cgcaccgatg cggcatatga tgtgattggc gtgcttggat
     3061 ggaacgacgc gccgatccag cggggccgcg gctccgcgat cttcctgcat gtcgcgcgcc
     3121 cggactatgc cccgacggac gggtgcatcg cgctggcaca gaacgatctg cggcaggtgc
     3181 tggcggctgg gacaaccgag gtcgtggtat cgggttaagc ccggtcgcgg ttcatgccgc
     3241 gaagttccag tgccgccagg taatcggacc accgcctgtc gtgctcgcgg ccgagctcga
     3301 gcaggtagtc ccacgaatag atgcctgtgt cgtgccggtc atcgaacacc agtcgcaccg
     3361 cgtagttgcc cacaggctct atcgaggtga ctgcgacatt gcgctttccg gccacgatct
     3421 ttttctgatc agggccgtgg ccctgcacct cggcgctggg gctttcgacc cgtagatatt
     3481 ccaccggcag ggcgaaggct gacccatcgt cgaatgcgac gtgcagcacg cgctccgccc
     3541 ggttcaggcg gatctcggtg ggaacagccg gcatcactcc tccagcttgc gtgcctcttc
     3601 cggcagcatg atcgggatgc cgtcgcgaac cggataggcc aggccggcct tccggctgat
     3661 cagttcgcca gcatcgcggt catattccag cgggcccttg gtcagcgggc acaccaggat
     3721 ttccagcagc cgaggatcga gcggcggctg tggcctggtt tcggtcggtt catccatgcc
     3781 gcctgctcct gtcagcttac gctgcgtcca cccgaggagg tatcgggggc cagcgcgccc
     3841 atctgcaaca gcaccagcag ggccgcgcca cgctcagcct gggttggtgc ttcaagcaac
     3901 gcctgtttct cggccggttc aaatgggcac accatggcca gcgtgatcac cagcatgtcg
     3961 tcgtccaggc gcttgatggc gtcccagttg gcgtcgaagc cgcggagggt gaagtagccg
     4021 cgcagcgcgg tcagcaatgc ctctcggtcc acgccgactg gctgcgccgt gagatcgagg
     4081 tcgccgtggt aggccgagaa gtcgccggcg acgcg
//
LOCUS       DATGEM010000459         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_922124, whole genome shotgun sequence.
ACCESSION   DATGEM010000459 DATGEM010000000
VERSION     DATGEM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922124"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VKQ27_14500"
     CDS             <1..1114
                     /locus_tag="VKQ27_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HLY90188.1"
                     /translation="AALEDAWPDVALEGVVVTRYGHSVPTRRIEVIESSHPVPDANSE
                     RGARRLLEQVRGLGPDDLVLALISGGGSALCAAPAAGLTLADKQAVNRALLASGANIT
                     EMNCVRKHLSAIKGGRLAAAARPARVVTLAISDVPGDDPAVIASGPTVPDPTTFADAR
                     AIVARYGIDVLPGVAARLNQDADETPKPGDLPNSEFRMIATPMIALNRVSETARSLGL
                     EPVVLGDALEGESQQMGIVMAGIARSVRTHRRPVAAPAVILSGGETTVTLGKGSAGRG
                     GRNTEFLLSLAVALGGAAGIWAISGDTDGLDGMDDIAGAIVAPDTLARARAKGLDPRA
                     MLAGHDSHSLFDALGDAVRTGPTLTNVNDIRAILIA"
     gene            1126..2559
                     /gene="pyk"
                     /locus_tag="VKQ27_14505"
     CDS             1126..2559
                     /gene="pyk"
                     /locus_tag="VKQ27_14505"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLY90189.1"
                     /translation="MATRPKLRRRRRTKIIATLGPASSSPEVMARLFQAGADVFRLNF
                     SHGTHEDHAARFALIREMEERFQRPIGILADVQGPKLRVGNFSGGRVFLQTGQRFQFD
                     LNPTPGGAGRVNLPHPEIIEAASIGSTLLLDDGKLKMRVVRKQGDALETEVVVGGPLS
                     NHKGVNVPDVVLPIPALTEKDHQDLAFALQHGANYIGLSFVQRPEDVVEAKKLIGGRA
                     WVMVKMEKPQALENLDAIMDLTDAVMVARGDLGVELPPEEVPLAQKRIVRLARRLGKP
                     VVVATQMLESMISAPAPTRAEASDVATAVFDGADAVMLSGETAAGQYPYEAVNIMDRI
                     VARVEQDSGWRAMIDASRPEPEHATADAIAAAARQVCHTIGAQAVVAFTASGSTALRV
                     ARERPDAPIIGLTPHLSIARRMAVVWGVHAVVSQDAHSMTEATTRATRVAQTEGFAKH
                     GDEIVVAAGVPFGHSGTTNSLRVATVR"
     gene            complement(2676..3275)
                     /locus_tag="VKQ27_14510"
     CDS             complement(2676..3275)
                     /locus_tag="VKQ27_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HLY90190.1"
                     /translation="MRLEFHFDFGSPNAYLSHLVIPGIEARTGARFEYVPILLGGVFK
                     LTNNRSPAETLAGIKNKPEYQQLETARFIERHGITRYQRNPFFPVNTLTLMRGAIAAQ
                     RLGIFDRYVDEVYRHMWADPKKLDDPAVLRSALTESRLDAAAILDLVQTTEVKEALLA
                     NTVRSVERGTFGSPTFFVEDAIYFGKDRLRDVEDAITGH"
     gene            complement(3275..3997)
                     /locus_tag="VKQ27_14515"
     CDS             complement(3275..3997)
                     /locus_tag="VKQ27_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90191.1"
                     /translation="MEQRTVAVIGAGDFIGSAIARKFAAQGFTVFAGRRNGEKLAELV
                     KESDGRILGRSVDARQEDDITAFLREADSFAPLEVCIFNPGANVHFPLVETTERVFRK
                     VWEMACYGGFLTGREAARLMLPRGRGAIFFTGATASLRGGAGYVAFASAKAGLRAVAQ
                     SAARELGPKGIHVAHLVIDSGVDTEWVRNRIREREGEAGLQNPDRLMQPASVGEAYWQ
                     LYQQPRDAWTFEMDIRPFGEKW"
     gene            complement(4009..5568)
                     /locus_tag="VKQ27_14520"
     CDS             complement(4009..5568)
                     /locus_tag="VKQ27_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY90192.1"
                     /translation="MSTTTADIRILPASELAMSTKVFAFASMSIGFFIALLDIQIVSA
                     SLADIGGGLSAGTDEIAWVQTSYLIAEIVVIPMSGWLTRVFSTRWLFCASAAGFTLTS
                     LLCGWAWNIESMIAFRALQGFLGGSMIPTVFTTAFYYFNGQQRVIAAATIGCISSLAP
                     TLGPTIGGWITDNYSWHWLFFINLVPGIFVTLVVPMLVRVDRVNLSLLRGADYVGMAL
                     MAVFLGCLEYTLEEGPRWDWFGDDTIRTTAWISGIAGIGFLWRSLSYTEPVVDLRALK
                     DRNFALGCFFSFVTGIGIFATIYLTPLFLDRVRGFSALQIGEAVFTTGLFQVLAIPLY
                     AVLTRFIDLRWLMMFGLACFGFSMWNFTPITHDWGAEQLLLPQAFRGFCQQFAVAPVV
                     TLTLGGIPPERLKFASGLFNLMRNLGGAIGIATCGTILNDRTNLHFLRLAEHLNSTNT
                     AMEQWLGEVGGTSGHVVALKRLWALTMREAQTQTFADAFFVIMLCFVVATVMVPLMRK
                     VALVAKPADAH"
     gene            complement(5565..6656)
                     /locus_tag="VKQ27_14525"
     CDS             complement(5565..6656)
                     /locus_tag="VKQ27_14525"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLY90193.1"
                     /translation="MSDQAVAARGDILAPPPVRAARVSRKQLLIAAAALALGVGAAAY
                     GYNYWTVGRFIESTDDAYVGGDVTVIAPKVPGFIAEVAVTDNEAVHAGDLLVKLDDRD
                     YRAALAKAEAAVAGQQATLENLAAQRSLQQAVIAQAQAELTATAAEILRSKADVDRYR
                     VLAQDHAASLQRSQQADADYQKALAADQKAHAALEASQRQLAVIDTQRQQTTAALAAA
                     MADRDTAQLNLSYTELRAPIDGTIGNRSARDGAYATVGTQLISLVPAHGLWVDANFKE
                     NQLAHMHAGQPVTIEADVLPDHTFHGHVASLAPATGAVFSVLPAENATGNFTKIVQRV
                     PVRVVLDQEAAELGLLRPGLSVTAGVDER"
     gene            complement(6653..7102)
                     /locus_tag="VKQ27_14530"
     CDS             complement(6653..7102)
                     /locus_tag="VKQ27_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY90194.1"
                     /translation="MQRKSFGTMSCPIARSLERVGEWWSILILRDAFRGLTRFDQFQA
                     SLGIAPNMLTRRLNALVESGLLGKRAYSEHPPRYEYVLTERGRDFRPVLWALLAWGNK
                     HFAPEGPSVVVVDSKTGAVADPVLVDRNSGRPLVEPDFRSHYLRPQP"
     gene            complement(7211..8293)
                     /locus_tag="VKQ27_14535"
     CDS             complement(7211..8293)
                     /locus_tag="VKQ27_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90195.1"
                     /translation="MPETGDARGDYPGLSYRNDADLAAWLARRPREAALEPDLPIIDP
                     HHHFWHTPQRGRYFLPELLADLGGGHSIIATVFLECQAMYRKHGPAEMAPVGEVEFVN
                     GIAAMSASGTYGPCRVAEAIIGHADLTLGARVRDVLEAQIRASGGRFRGIRYGVSHDA
                     SPAISKFVSRTVPPHLVRDPTFRQGFAQLAPLGLSFESWQYHPQLPDAIDLARAFPDT
                     TIILNHVGGVLGVGPYAGHREEIRAEWRKNIVELAKCPNVNVKLGGIGMTSFGFNFHE
                     RGVPPTSEELAAAWRQYVEPCIQAFGPNRAMFESNFPPDKQSCGYTELWNAFKRITAG
                     ASAADKSALYAGTAARVYRLQNPSPA"
     gene            complement(8463..8660)
                     /locus_tag="VKQ27_14540"
     CDS             complement(8463..8660)
                     /locus_tag="VKQ27_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90196.1"
                     /translation="MRRASLLPALLLLGLTGCVVATPTPSTTTYVTPAPAPTTTYVTP
                     APTAAYVTPAVPTTTTVIGTP"
BASE COUNT         1568 a         2667 c         3225 g         1610 t
ORIGIN      
        1 ggcggcgttg gaggatgcat ggccagacgt ggccctggag ggcgtcgtgg tgacccgcta
       61 tggccacagc gtgccgacgc ggcggatcga ggtgatcgag tcatcgcatc cagtgcccga
      121 tgccaacagc gagcggggag cccgcaggct gttggagcag gtgcgagggc tggggccaga
      181 cgatctggtg ctggcactga tctcgggtgg cggttcggcg ctgtgcgcgg cgcccgcggc
      241 gggcctgacg ctggccgaca agcaggcggt gaaccgggca ctgctggcaa gcggggcgaa
      301 catcacagag atgaactgcg tgcggaagca cctgtccgcg atcaagggtg gcaggctggc
      361 ggctgcggcg cggccggcgc gggtggtgac gctggctatc agcgacgtgc cgggcgatga
      421 tccggcggtg atcgcttcgg ggccgacagt cccggatcca acgacattcg cggacgcccg
      481 ggcgatagtg gcgcgctatg ggatcgatgt gctgccgggt gtggcggcgc ggctgaacca
      541 ggacgcggat gagacaccga aacccggcga tctgccgaac agcgagttcc ggatgatcgc
      601 gacgccgatg attgcactga accgggtgtc ggagaccgcg cggtcactgg ggctggagcc
      661 ggtggtgctt ggcgatgcgc tggaaggcga gtcgcagcag atgggcatcg tgatggccgg
      721 catcgcgcgc agcgttcgaa cgcaccgccg gccggtcgcg gcgccggcgg ttattctttc
      781 tggtggtgaa acgacggtga cgctgggcaa gggttccgcc ggtcgcggtg gacgtaacac
      841 agaattcctg ctgtcactcg ccgtcgcatt gggaggggcc gcgggaatat gggccatatc
      901 aggggatacc gatggattgg acggcatgga tgacatcgcc ggcgccatcg tcgctccgga
      961 tacgctggcg cgcgcccgtg cgaaggggct cgacccgcgt gcgatgctgg ctggacacga
     1021 cagccactcg ctgttcgatg cgcttggaga tgcggtccgc accgggccca cgttgaccaa
     1081 cgtgaatgac atccgcgcga ttctgatcgc ctgaaggagc ccggcatggc cactcgaccg
     1141 aagttgcgtc ggcgccggcg caccaagatc attgccacgc ttggaccagc cagttcgtca
     1201 ccggaggtga tggcgcggtt gtttcaggct ggcgcagatg tgttccggct gaatttctcg
     1261 catggcacgc atgaggatca tgcggcgcgc tttgccctga tccgcgagat ggaggagcgg
     1321 ttccaacgac ccatcgggat cctggccgac gtgcaaggac caaagctgcg ggttggcaat
     1381 ttctccggcg ggcgcgtgtt cctgcagacc gggcagcgat tccagttcga cctcaatcca
     1441 acgccgggcg gcgccgggcg ggtcaatctg ccacatccgg agatcatcga ggccgcatcg
     1501 atcggctcca cgttgttgct tgacgatggc aagctgaaga tgcgcgttgt tcgcaagcag
     1561 ggcgatgcgt tggagacaga ggtggtcgtg ggtggaccgc tctcgaacca caagggtgtc
     1621 aatgtgcccg atgtggtgct gccgattccg gctttgacgg aaaaggatca ccaggacctg
     1681 gcgttcgcgc tgcagcatgg cgcgaattac atcggcttgt cgttcgtgca gcggccggaa
     1741 gatgtggtgg aggcgaagaa gctgatcggt ggccgcgcct gggtgatggt gaagatggag
     1801 aagcctcagg cgctggagaa cctggacgcc atcatggatc tgaccgatgc ggtgatggtg
     1861 gcgcgcggcg atctcggcgt ggagctgccg cccgaggaag tgccattggc gcagaagcgg
     1921 atcgtgcggc tggcgcggcg gttgggcaag ccggtggtgg tggcgactca gatgctggag
     1981 agcatgatca gcgcgccggc accgacgcgc gccgaggctt ctgatgtcgc gacggcggtg
     2041 ttcgatgggg cggatgcggt gatgctgtcg ggggagacgg cggcggggca gtacccgtat
     2101 gaggcggtca acatcatgga ccgtatcgtc gcgcgggtgg agcaggactc cggctggcga
     2161 gcgatgatcg atgcgagccg gccggaaccg gagcacgcaa cggcggacgc gatcgctgcg
     2221 gcggcacggc aggtgtgcca cacgatcggc gcgcaggcgg tggtggcgtt caccgcgtcc
     2281 gggtccacgg cgctgcgtgt ggcgcgcgag cggccggatg cgccgatcat tgggctgacg
     2341 ccgcatttga gcatcgcacg gcggatggcg gtggtgtggg gcgtgcatgc cgtggtgtcg
     2401 caggacgcgc actcgatgac ggaggcgacg acgcgggcta cgcgggtggc gcagactgag
     2461 ggattcgcca agcatggcga tgagattgtc gttgcggcgg gtgtgccgtt cggacattcg
     2521 gggacgacga attcgctgcg ggtggcgacg gtgaggtagg gtgcgcgctt ggattgccag
     2581 gggagtactc gttcgagatt gcttcgcccg gctttcgccg gggtcgcaat gacaggaggg
     2641 ggcagggggg acaagagggg acagggtggg agaggtcagt ggcccgttat ggcgtcctcg
     2701 acatcgcgta gccgatcctt gccgaaatag attgcgtctt cgacgaagaa cgtcggcgag
     2761 ccaaaggtgc cgcgttccac cgaacgcacg gtatttgcca gaagcgcttc cttcacctcg
     2821 gtggtctgca ccaggtcgag aatagctgcc gcatccagcc tggattctgt cagcgccgaa
     2881 cgcagcaccg cggggtcatc cagctttttc gggtctgccc acatgtggcg gtagacttca
     2941 tccacgtagc gatcgaagat acccaatcgc tgggcggcga tcgcgccgcg catcagggtc
     3001 agcgtgttca ccggaaagaa tgggttgcgt tggtaccggg ttatcccgtg gcgctcgata
     3061 aaccgagccg tctccaactg ctggtattcc ggcttgttct tgatccccgc gagtgtctcg
     3121 gcgggtgaac ggttgttggt gagtttgaat acgccaccca gcaggatcgg cacgtactcg
     3181 aatcgcgcac ctgtccgcgc ctcaatacct gggatgacca gatggctgag ataggcgttc
     3241 gggctgccga aatcgaagtg gaattcgagc cgcatcacca tttctctccg aacggccgga
     3301 tgtccatttc gaatgtccag gcatctctcg gctgctgata caattgccaa tacgcttcgc
     3361 ctaccgaggc cggctgcatc aggcgatcgg ggttctgcaa tccggcctcg ccttcgcgtt
     3421 cgcggatgcg gttgcgcacc cattcggtgt ccacgccgga atcgatgacc agatgcgcca
     3481 cgtgaatgcc cttgggtccc agttcccgcg cggcactttg cgcgacggcg cgcagccctg
     3541 ctttggccga ggcgaatgcc acgtatccgg cgccaccgcg caggcttgct gttgcgccgg
     3601 tgaagaagat tgcgccgcgt ccgcgcggca gcatcaggcg ggccgcttcg cgcccggtca
     3661 ggaagccgcc gtagcacgcc atctcccaaa ccttgcgaaa cacgcgttcg gttgtttcca
     3721 ccaaggggaa atggacattg gcgccggggt tgaagatgca cacttcgagc ggcgcgaatg
     3781 aatcggcttc gcgcaggaag gcagtgatat cgtcctcctg ccgcgcatcg accgagcggc
     3841 cgaggattcg cccgtctgac tccttcacca gttctgcgag cttctcacca ttgcgtcgac
     3901 ccgcgaacac ggtgaagccc tgggcggcaa atttccgggc gattgcgctg ccgatgaaat
     3961 cgcctgcgcc gatgactgcg actgtgcgct gttccatctg tccctccatc agtgagcgtc
     4021 cgccggcttc gctaccagcg cgaccttccg catcagcggc accatcactg tggcaaccac
     4081 gaagcacagc atgatgacga agaacgcatc ggcgaaggtc tgtgtctgtg cctcgcgcat
     4141 ggtcagcgcc cagaggcgct tcagcgccac tacgtggcca cttgtgccgc ctacctcgcc
     4201 gagccattgc tccatcgctg tgttggtgct gttcaggtgc tcggcgaggc gtaggaagtg
     4261 caggttggtg cggtcgttca ggatcgtgcc gcaggtcgcg atgccgatgg caccacccag
     4321 gttgcgcatc aggttgaaca gcccggaggc gaatttcagc cgctctggcg ggatgccacc
     4381 cagggtcagc gtcaccaccg gtgccacggc gaactgctgg cagaagccgc ggaacgcctg
     4441 gggcagcagc aattgttccg cgccccaatc atgcgtgatc ggggtgaaat tccacatgct
     4501 gaagccgaag caggccaggc cgaacatcat cagccagcgg aggtcgatga agcgggtcag
     4561 cacggcgtaa agtggaatgg caaggacctg gaacaggccg gtggtgaaca ccgcttcgcc
     4621 gatttgcagc gcactgaagc cacgcacgcg atcgaggaac agcggggtca ggtagatcgt
     4681 cgcgaagatg ccgatgccgg tgacgaagga gaagaaacag ccaagcgcga agttgcgatc
     4741 tttcagcgcg cgcaaatcga ccacgggctc ggtgtagctc aggctgcgcc agaggaagcc
     4801 gatgccggct attccggaaa tccaggcggt cgtgcggatg gtgtcatcgc cgaaccagtc
     4861 ccagcgtggt ccttcctcca gcgtgtattc gaggcagccg aggaacactg ccatcagcgc
     4921 catgccgaca tagtcggcgc cgcgcaggag cgacaggttc acgcggtcca cccggacgag
     4981 catcggcacg acgagggtga cgaaaatgcc ggggaccagg ttgatgaaga acagccagtg
     5041 ccatgagtaa ttgtcggtga tccagccacc gatcgtcggg cccaatgtgg gggcgagcga
     5101 cgaaatacag ccgattgtcg cggccgcgat cacgcgctgc tggccgttga agtaatagaa
     5161 cgcggtggtg aacacggtcg ggatcatcga gccaccaaga aagccctgca gggcgcggaa
     5221 agcgatcatg ctttcgatgt tccacgccca gccgcacagc agacttgtca gcgtgaagcc
     5281 tgccgcggat gcgcagaaca gccagcgcgt ggagaacacg cgggtcagcc agcctgacat
     5341 cgggatcacg acaatctcgg cgatcaggta gctggtctgg acccaggcga tctcgtcggt
     5401 gccagcggac agcccgccgc cgatatccgc cagcgacgcc gagacgatct ggatgtcgag
     5461 gagtgcgatg aagaagccga tactcatgga ggcgaaggcg aacaccttcg tcgacatcgc
     5521 cagctcggaa gccggcagga tccggatatc ggctgtggtg gtgctcatcg ttcgtcaacg
     5581 ccggcggtta cggagaggcc agggcgcagc aggccaagtt cggcggcttc ctgatccagc
     5641 acgacgcgta ccggcacgcg ctgcacgatt ttggtgaagt tcccggtcgc attctctgcc
     5701 ggcagcacgc tgaacactgc gccggtggcg ggggccaggc tcgcaacgtg gccatggaag
     5761 gtatggtctg gcagcacgtc ggcctcaatg gtcaccggct ggccggcgtg catgtgcgcg
     5821 agttggtttt ccttgaagtt ggcatccacc cagagcccgt gggccggcac cagcgagatc
     5881 agctgtgtgc ctacagttgc ataggcacca tcccgcgcgc tgcggttgcc gatggtaccg
     5941 tcgattggcg cgcgaagttc ggtgtagctc aggttgagtt gggcagtgtc gcgatcggcc
     6001 atggcggcag cgagggcagc cgtcgtctgc tgccgttgcg tgtcgatcac tgcgagctgg
     6061 cgctgagaag cctccagggc ggcgtgcgcc ttttggtcgg ctgccagggc cttctggtaa
     6121 tcggcatcgg cctgctgcga acgctgtagc gaggcggcgt ggtcctgcgc caggacccgg
     6181 tagcggtcga cgtctgcctt cgaacgcagg atctcagccg cggtcgcggt cagttcggcc
     6241 tgcgcctggg cgatgacggc ctgctgcagg ctgcgttgcg ccgcaaggtt ctcgagcgtt
     6301 gcctgttggc cggcgaccgc tgcctcggcc tttgccagcg cggcccggta gtcgcgatcg
     6361 tccaatttca ccagcaggtc gccggcgtgg actgcctcgt tgtccgtcac cgcgacctcg
     6421 gcgatgaagc cgggcacctt cggcgcaatc accgtgacgt cgccgccgac ataggcgtcg
     6481 tcggtgcttt cgatgaaacg gccgacggtc cagtaattgt atccgtaggc ggcggcgccg
     6541 accccaagtg ccagggcggc cgcggcgatg aggagctgct tgcgcgagac ccgggccgcg
     6601 cgcaccggcg gcggtgcgag gatgtcgcca cgggcggcaa cggcctggtc gctcatggct
     6661 gcggccttag gtagtgcgag cggaagtcag gttcgaccaa agggcgcccg ctgttcctgt
     6721 ccaccagcac cggatcggca actgcgccgg tcttgctgtc cacgaccacc acgctggggc
     6781 cttccggcgc gaagtgcttg ttgccccagg cgaggagcgc ccacagcaca gggcggaagt
     6841 cgcggccgcg ttcggtcagg acatactcgt agcggggagg gtgctcgctg tacgcgcgct
     6901 ttcccaacag gccgctttcg accagcgcgt tcaggcgcct ggtcagcatg ttgggagcga
     6961 tgcccaggct ggcctggaac tggtcgaagc gggtgaggcc gcggaaggcg tcgcggagga
     7021 tcaggatgct ccaccattcg cccacccgct ccaggctgcg ggcgatgggg cacgacatcg
     7081 tgccgaagct cttgcgttgc atgtctctgc gtcccgtgac ttgcaggatg caagctacat
     7141 aggccagtga cttgcaagat gcaagggacg gggtgccggt tcggacgcac cccctgccgg
     7201 cccccctcgt ttaagcgggg gaggggtttt gcaggcggta aacgcgggcg gcggtgccgg
     7261 cgtagagagc tgatttatcg gcagccgatg cgccggcggt gatgcgcttg aaggcgttcc
     7321 acagttcggt gtagccgcag gactgcttgt cgggcgggaa attgctctcg aacatggccc
     7381 ggttcggccc gaaagcctgg atgcatggct cgacgtactg gcgccaggcg gcggcaagct
     7441 cctcggaggt cggcggcacg ccccgttcgt ggaagttgaa gccgaacgac gtcatgccga
     7501 tgccgccgag cttcacgttc acgttcgggc acttcgccag ctcgacgatg ttcttgcgcc
     7561 attcggcacg aatttcctcg cggtggccgg cgtaggggcc gacgcccagc acgccaccaa
     7621 catggttcag gatgatcgtc gtgtcgggga aggcgcgcgc gagatcgatc gcgtcgggta
     7681 gttgcggatg atactgccag gactcgaagc tgagcccgag cggcgccagc tgtgcgaagc
     7741 cttgccggaa cgttggatcg cgcacgaggt gtgggggcac cgtgcgggac acgaacttgc
     7801 tgatcgccgg gctggcatcg tgtgaaaccc cgtagcgtat gccgcggaac cgtccgccgc
     7861 tggcacggat ctgcgcctcc agcacgtcac gcacgcgagc gccgagtgtc aggtcggcgt
     7921 ggccgatgat tgcctctgcg acgcgacatg ggccgtaggt gcctgacgca ctcatcgccg
     7981 cgatgccgtt gacgaattcg acttcgccga cgggggccat ctcggcgggg ccgtgtttgc
     8041 gatacatcgc ctggcattcc aggaaaacgg tggcgatgat gctgtggccg ccgccaaggt
     8101 ctgcgagcag ctcgggcagg aagtagcggc cgcgctgggg cgtgtgccag aaatggtggt
     8161 gcggatcgat gatcggcagg tccggttcca gtgctgcctc gcgtgggcgc ctggcaagcc
     8221 aggcggcgag atccgcatcg ttgcggtagc tcaggccggg gtagtcgccg cgcgcatcgc
     8281 ctgtttcggg catggtgttc ctccggttga tgggggagat cctatcatcc gaaggcgggc
     8341 ggctgcgaaa tggtgggtcg ggcgggcgga ccaggctgcc gcaatgcaag gggctcgcgg
     8401 cccacgatat ccgccgttat cgtgggcctg gccgagggtc gagcctggct tgtgctgcga
     8461 ggttatggcg tgccgatcac cgttgttgtc gtgggcaccg ccggcgtcac atacgctgcc
     8521 gtcggagccg gcgtgacgta ggtggtggta ggcgccggtg caggggtcac gtaggtcgtt
     8581 gttgacggtg tcggcgtggc cacgacgcat ccggtcaggc cgagcaagag taacgctggc
     8641 agtagggaag cacgacgcat cataacggtc tcctctcagt gcgagagcgt caggaaggcg
     8701 ctcgaatctg gcgaaacatt gcctatagac aatcacgggt tggcgaccgg tattactttg
     8761 ctgtgaacgc tggggactgc ggccgctcca ctaatgccaa tgctcgatga cgacgatgca
     8821 ttgtcacagg catgaagacg tggatgccgg gccttcgccg ggcatgacgg gagcattgat
     8881 cggtcccact caatgatatg tggtatgatg ccggcagaat ttcgcggccc gggagcttgc
     8941 gctgggtggg tctcggtatt ccgaaatggg acactgtcac ccgcgctgac atgtggcgcg
     9001 acccatgcta cacaacgccg ccgctttggc actggatgcg cccttagctc agttggatag
     9061 agcaacagcc
//
LOCUS       DATGEM010000460         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_801730, whole genome shotgun sequence.
ACCESSION   DATGEM010000460 DATGEM010000000
VERSION     DATGEM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801730"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VKQ27_14545"
     CDS             complement(<1..1310)
                     /locus_tag="VKQ27_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HLY90197.1"
                     /translation="MSDLPTATRVSDPGIRSLYRLENRWQAWLDVEVALARAQAELGI
                     IPHDAAESIARSARLELLDRGRIDEGFARTGHTIVPLVWELARVVGEPHGGWVHWGAT
                     TQNITQTGDLLVLRQAHGVFVRLIGEALTAMADLAERGAEMPVPGRTHGQHAVPATFG
                     YKVAVWVDELLRHVERLRQAGPRIFVAMLGGGAGTFASLGKQGPPVQAGIGRQLGFGT
                     MAVPSRALGDHLAENICILGMLAATCSKIGREIYTLMKTEFGEVEEPVPPGTVGSSTM
                     PQKRNPKLCQDIIAAAAEVRAMVPLALEAMQTEHEADRTTSLIMDSAESRACIGTGDM
                     LARLGEVMRGLKLDPARMRRNLDLGGGLIMAEAVMLDLGATIGRQHAHDVVYDAAQAA
                     AVEGKSFGDLLAADKRVTAHLDAKAIAALLDPTAYTGLCADMAR"
     gene            1457..2722
                     /locus_tag="VKQ27_14550"
     CDS             1457..2722
                     /locus_tag="VKQ27_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLY90198.1"
                     /translation="MTEAIQLTRLPSGLTVVTERMERVETVSLGAYVATGSRHEQAAE
                     NGVSHFLEHMAFKGTERRTAAQIAEEIEAVGGHINAYTAREQTAYYVKVLKEDTALAA
                     DIIGDILTHSTFEPEELERERGVILQEIGQANDTPDDIIFDHFQEHAYPEQPLGRPVL
                     GSEEGIQSMPRAMLTGYMKRNYAASNIVVAAAGALRHEQILDLVNMHFADLPGERAPA
                     MPGAQYAGGEFREARDLDQVHIVLGFPSVSYTDRDYYPTLLLSTLLGGGMSSRLFQEV
                     REKRGLVYSIYSFSSPFLDGGLFGIYAGTGESEAEELMPITLEELRKVQTAVTEVELA
                     RARAQVKASLLMSLESTGSRCEQLARQLQVFGRVVPTNETVAKLNAVTTADVCRAASK
                     LFRGSPTLATMGPAERVPKLVDIAEKLAA"
BASE COUNT          516 a          998 c          885 g          467 t
ORIGIN      
        1 tcacgcgcca tgtccgcaca gaggccggta taggcggttg gatccagcag cgccgcgatc
       61 gccttggcat cgagatgcgc cgtcacccgc ttgtcggcgg ccaacaggtc gccgaacgac
      121 ttgccctcca ccgccgcggc ctgtgccgcg tcgtaaacca cgtcatgcgc gtgctgccgg
      181 ccaatggtgg cgccgaggtc aagcatgacc gcctcagcca tgatcaaccc gccgcccaga
      241 tcgaggttcc gccgcatgcg cgccggatca agcttcaacc cccgcatcac ctcgccaagt
      301 cgcgccagca tgtcgccggt cccaatgcag gcgcgcgact cggctgaatc catgatcaga
      361 ctagtggtcc gatcggcctc atgctcggtc tgcattgcct cgagtgccag cggaaccatc
      421 gcgcgcacct cggccgcggc ggcgatgatg tcctggcaca gcttcggatt gcgcttctga
      481 ggcatcgtcg agctgccgac ggtaccgggc ggcaccggtt cctccacctc gccgaattcc
      541 gtcttcatca acgtatagat ctcgcggccg atcttgctgc aggtggcagc aagcatgccg
      601 agaatgcaga tgttctcggc cagatggtcg ccgagcgccc gcgacggcac ggccatcgtg
      661 ccgaaaccga gttgcctgcc gatgcccgcc tgcaccggcg gcccttgctt gcctagcgac
      721 gcaaatgtcc ccgcgccacc acccagcatc gcgacgaaaa tacgcggccc tgcctgccgc
      781 aggcgctcaa cgtgacgcag caattcatcg acccacaccg cgaccttgta gccaaatgtg
      841 gccggcacgg cgtgttggcc gtgcgtgcgg ccaggcaccg gcatctcggc accccgttcg
      901 gcgagatcgg ccatcgcggt cagcgcctca ccgatcaggc gcacgaacac gccgtgcgcc
      961 tggcgcagta ccagaaggtc gccggtctgc gtgatattct gtgtcgtggc gccccagtgc
     1021 acccacccgc catgcggctc gccaaccacc cgtgccagct cccagaccag tggaacgatg
     1081 gtatggccgg tgcgcgcaaa cccttcatcg atgcggccgc gatccagcag ctccaggcgc
     1141 gccgagcgcg caatcgactc cgccgcgtcg tgcggaatga tccccagctc ggcctgggca
     1201 cgcgccagtg cgacctcgac gtccagccac gcctgccagc ggttctccag gcgatagaga
     1261 gaccgaattc ccggatcgga gacgcgcgtg gcggttggca gatcactcat ggcagtggcc
     1321 tcccggcagc gtttgctcct ggcagtctgg tgcctcccgg gaatgccggc aagccggtcg
     1381 cccattgaaa ccgcgcccgg gaaagggcag ttacaggtcc ctgttcatcc gatatttgcc
     1441 aggaatgtcg cccccagtga ccgaagccat ccagctgacc cgcctgccct ccggcctgac
     1501 cgtcgtcacc gaacggatgg agcgggtcga aaccgtctca ctgggagcct acgtcgcgac
     1561 cggctcacgc cacgagcagg cagccgagaa cggggtctcg cacttcctgg aacacatggc
     1621 cttcaagggc accgaacgcc gaaccgctgc tcagattgcc gaggaaatcg aggctgtcgg
     1681 cggccatatc aacgcctata ccgcccgcga gcagactgcc tattacgtca aggtcctcaa
     1741 ggaggacacc gcgctggccg ccgacatcat cggcgacata ctcacccact ccaccttcga
     1801 acccgaggaa ctggagcgcg aacgcggcgt catcctccag gagatcggcc aggcgaacga
     1861 cacgccggac gacatcatct tcgaccactt ccaggagcac gcctatcccg agcagccgct
     1921 cgggcgtccc gtgctgggtt ccgaggaagg catccagtcc atgccacgcg ccatgctgac
     1981 cggctacatg aagcgcaact atgccgcctc caacatcgtg gtggcggcgg cgggcgcact
     2041 ccgacacgag cagatcctcg atctggtgaa catgcacttc gccgatcttc ccggcgaacg
     2101 cgcgcccgcc atgccagggg cgcaatatgc tggcggcgag ttccgtgagg cgcgcgatct
     2161 cgatcaggtc cacatcgtgc tgggcttccc ttcggtcagc tacaccgacc gcgactacta
     2221 cccgaccctg ctgctttcca ctttgctggg cggcggcatg tcgtcgcggc tgttccagga
     2281 agtgcgggag aagcgcggac tggtctattc aatctactcg ttcagctcgc cgttcctgga
     2341 tggcggactg ttcggcatct atgccggcac cggcgagagc gaagctgaag agctgatgcc
     2401 gataacgctc gaggaactgc gcaaagtgca aaccgcggtg accgaggtcg agctggcgag
     2461 ggcgcgggca caggtgaagg catcgctgct catgtcgctg gaaagcaccg gcagccgttg
     2521 cgagcagctt gcccggcagt tgcaggtgtt cggccgtgtg gtgccgacga acgagaccgt
     2581 ggcgaagctc aacgccgtga ccaccgccga tgtctgccgc gctgcctcga aactgttccg
     2641 cggaagcccg acgctggcga ccatgggacc ggcggaacgt gtgcccaagc tggtggacat
     2701 tgcggagaaa ctggccgcct gattggcaag gagccatgtg atggctgagc cacttaacct
     2761 tctgtccgat ctgatcgcgc gggcacgcgc tgccggcgcg gacgccgccg atgccgtgct
     2821 gatctccggc acgtcgctgt ctgtgcagca gaggctgggc aagacc
//
LOCUS       DATGEM010000461         9152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_791310, whole genome shotgun sequence.
ACCESSION   DATGEM010000461 DATGEM010000000
VERSION     DATGEM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791310"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VKQ27_14555"
     CDS             complement(<1..297)
                     /locus_tag="VKQ27_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90199.1"
                     /translation="MPRRLSRRAARCGRMVLAQRPWSAAGQGTVLAWAQRAAARYGAR
                     AAVAAAAEVVLPRVVAAAAHPGRVAVLQAVAARVRLSVAAFVVRLQVAAVQAVSR"
     gene            306..590
                     /locus_tag="VKQ27_14560"
     CDS             306..590
                     /locus_tag="VKQ27_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLY90200.1"
                     /translation="MSPNWQGALAAWLETHKTYPEQARRRGEQGRGSVRFTVDRSGQV
                     LDVAIVSSTGSSILDDAIERMLRGAHVPPFPANMDETQVTVTVQIRYALE"
     gene            complement(553..1137)
                     /locus_tag="VKQ27_14565"
     CDS             complement(553..1137)
                     /locus_tag="VKQ27_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLY90201.1"
                     /translation="MAGEFSRRTENLLDRAEAICEHRGARLTELRRHVLGLVLDAAAP
                     TGAYELLDRLRQTRRGAAPPTVYRALDFLLDQGLIHRVERLSAFVGCIAGCTADHDEG
                     EGEIHTHAAQFLICRQCGRVIEMQDHDVSSVLARAAKEAGFSISGATIEAEGLCSTCR
                     SSEVRKPTTPSRTAPPAGGRPGAIRGRSGSARSR"
     gene            1193..2059
                     /locus_tag="VKQ27_14570"
     CDS             1193..2059
                     /locus_tag="VKQ27_14570"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HLY90202.1"
                     /translation="MAKRLLHLILVCLFARYAAAQPLPIVAAENFYGDVARQIAGSDA
                     QVSSILTNPDQDPHLFEARPSVARQLATAAIVICNGADYDPWMTRLLAATHTPDRQVI
                     VVADIVHRKPGDNPHLWYDPPTMPAYASALATALIQRDPPHKAEYELRLQTFLTSLQP
                     LQRKIAEMHARLNGTPVTATEPVFSYMATALGLTMRNQRFQLAVMNDTEPRASDIAAI
                     DDDLREHRVHILFYNSQATDTAAQRLVAIAHQSGIPVVGVTETEPPDKTYQQWMTDQL
                     DAVAQALANHPS"
     gene            2056..2862
                     /locus_tag="VKQ27_14575"
     CDS             2056..2862
                     /locus_tag="VKQ27_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY90203.1"
                     /translation="MNAAEFSGTTLAFGDRTVLANVDLAIRDDEFIGVLGANGAGKTT
                     LMRAILGLVPVQRGAIRVLGAPVARGNPAVGYMPQTRAALDHVHLTGWDFVASGSAGH
                     RWGLPLLNRTMRRDVEWALERVAAKDLARRPLTETSGGERQRLLLAQALLGRPRLLLL
                     DEPLISLDPHHQTAVVELTRALQQELGIAVLFSAHELNPLLGALDRVLYLGRSHAALG
                     TVDEVITGPVLSRLYGSDIDVVRVNGRIFVMSGGHDVERDAHQHDHHVHV"
     gene            2849..3640
                     /locus_tag="VKQ27_14580"
     CDS             2849..3640
                     /locus_tag="VKQ27_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HLY90204.1"
                     /translation="MFTYDFMQNAFAAAGIVSVVSGLIGFFLVLRAQTFAGHALSHVG
                     FAGATGAVLFGLPPLWGLIALTLAAGVGMGLLGEQLYQRDVAIGIVLAMSLGLGLLFL
                     NFFTAYATQATALLFGNILGIDDTTVWTLLALGVAAIAALAAIARPLLFASLQPELAE
                     AKGVSLRLVSVLFLAITALGVAECAQIVGVLLVFALMVGPPAAAQRLTAHFWPGIMLS
                     ALLALAEAWLGLMLAYATDWPASFWITALSGAVYLLATLGIRSLR"
     gene            3881..4249
                     /locus_tag="VKQ27_14585"
     CDS             3881..4249
                     /locus_tag="VKQ27_14585"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HLY90205.1"
                     /translation="MRSVIRFTLMWLAAWCQVLVVATMPLAPLAMAADPLGEIPICHA
                     PNPADGQQTPSPEHGQHDCALCVMCCQVHASSAALLSPTPSLPERQTIAAIRHHTAQP
                     RAPPVRIAVAAQPRGPPSPT"
     gene            4301..5266
                     /locus_tag="VKQ27_14590"
     CDS             4301..5266
                     /locus_tag="VKQ27_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90206.1"
                     /translation="MPSPRCLAALTAASLLPVSSGWAHGFAGPHMFISTLLIDDPNVA
                     DEASLPTFFYLPQPTDGGTAPKLYNVSFEFDKRITENFGFSISDGYNWLRIPGVGTAS
                     GWQNLEATLKYKAYVNDEHEFMLSLGVSREFARTGATGANGATLDNDDSGSTTPTIYW
                     GKGFGDIPVPLLRPLALTGTLSYTIADNKLKITGTDPDSGNLLFNNGISNTWSGGLSL
                     QYSFRYLQTQVKDYGWPEFVNKLTPVVEVAWSSPASKPNQDSTQYLIGVGLNYTANSY
                     AVGVEALIPGNRQTGSHAGVIAQFHLYFDDLFPNSLGKPIVGWFQ"
     gene            5263..5622
                     /locus_tag="VKQ27_14595"
     CDS             5263..5622
                     /locus_tag="VKQ27_14595"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HLY90207.1"
                     /translation="MIRAAPWLSLAMLLIASPAFAHALLERAEPPVGSEITAPPHQVT
                     LRFTEGVEPVFSSIEIHDAQGALVPIGKPHTVPGNDRTLVVDLPDLRAGRYTVTWHVT
                     SVDTHKTEGSFQFSVIH"
     gene            5683..6129
                     /locus_tag="VKQ27_14600"
     CDS             5683..6129
                     /locus_tag="VKQ27_14600"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HLY90208.1"
                     /translation="MAASAEPASIKVEQVWSRAAVVGHEGVAYMIITNAGPTDALTAV
                     STPIAATADLHQTINDNGVMKMRPVQSLPIDAGKTVTLAPRGYHIMLTNLKQTLKEGD
                     SFPITLTFQKAGKITATAKVEQAGATSAPSSGASDHMNMPMSGSKP"
     gene            6159..7034
                     /locus_tag="VKQ27_14605"
     CDS             6159..7034
                     /locus_tag="VKQ27_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLY90209.1"
                     /translation="MEAADRAARSWRWPDPRPLRLGSEEHKQAACRMFRETFNPYKPA
                     VIDWPKLDPHALERLTRLPIWNIAVQTEGNARLRMAAYASSLLDPEWREAIGQNGWEE
                     GRHKDVLAKLVTAYGIRLEPEPPYLEPHDTEWAYLVTGFSECIDSFFAFGLFELARRS
                     GFFPIELVDTFEPVIQEEARHILLFANWLAWHRRRMPLWKRPWFEARVAAVWAFLVWE
                     RIGLARTVDGGGTDSNFTVNGSRSVSDAAIKPATLMRICLDENDRRFAGYDPRLLRPT
                     TVPRLVRFAQHFLRG"
     gene            7161..9041
                     /locus_tag="VKQ27_14610"
     CDS             7161..9041
                     /locus_tag="VKQ27_14610"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HLY90210.1"
                     /translation="MTPLDIVVASLRGLHLIALVSAFGTLTAIAMTPATTKESAYLHR
                     TLRRLVHLSTGCALITGIAWLTTESAAIAGTDTIATTLSAIPIVAFQTQYGRWFTARC
                     VLLLILLITPLARPTGLIAALILAGGALTIQPMVGHAGAIGGAVGTELIVSEALHLLA
                     AGAWLGGLLPLFLAVSILPHEAAATACRNFTPVGLSAVLILAGTAIVQITELMGGLPG
                     LFGTTYGHLALVKIAIFLVLLMLAALNRLVFTEHLAHSTAGVTQRHMRLSIALEMLLG
                     ASVVVAASFLATLTPGTHEQPVWPFPWRPSLFAFQDPLLRPEISTAAIATAIALALIV
                     FAVVWRRARWVALALAVVILAFALPHIDLLFIDAYPTSFFTSPTEFAATAIVHGARLF
                     AANCVTCHGADATGDGPNAKSLPIPPADLTAEHFWAHSDGELYWYIAHGFEAPGGGTA
                     MPGFSAALSTEAIWDLIDYLRANNGGASMRATGTWSHPLPVPQFDFRCADKRTLDLDD
                     MRGKPMRIIAASDDEPIVPLPDVTTISVVRSHAAAPAAGGCVTSEPETWTAFAILLGQ
                     SPATLDGWQILVDQNAWLRAAWHPGQPGDWTNPETLVRRIHDIASHPLDVILPGAHVH
                     IH"
BASE COUNT         1580 a         3471 c         2507 g         1594 t
ORIGIN      
        1 ccgcgacact gcctgcaccg ccgccacctg cagtcgaacc acaaacgccg ccaccgacag
       61 ccgcacccgc gccgccaccg cctgcaacac agccacccgc cccggatgtg ccgctgccgc
      121 caccacccgt ggaagcacca cctccgccgc tgccgccacc gcagcccgcg cgccatacct
      181 cgccgctgcg cgctgcgccc aggccaacac cgtcccctgt cccgccgcgc tccacgggcg
      241 ctgtgccagc accatccgcc cgcaccgcgc cgcccgccgc gacagcctcc gcggcacagc
      301 cagccttgtc gcccaattgg cagggcgcgc tcgcagcctg gctcgaaaca cataagacct
      361 atccagagca ggcccgccgc cgcggcgagc aaggccgcgg cagcgttcgc ttcaccgtcg
      421 accgcagcgg tcaggtgctc gatgtggcaa tcgtatcgtc caccggttca tccatcctcg
      481 acgacgcaat cgaacgcatg ctgcgtggcg cccatgtccc accgttcccc gccaacatgg
      541 acgagaccca ggtcaccgtg accgtgcaga tccgctacgc cctcgaatag ctcccggcct
      601 gcccccagcg ggaggtgcgg tgcgcgatgg tgtggtgggc ttccgcacct ctgacgatcg
      661 gcaggtgctg caaagcccct ctgcctcgat cgtcgcgccg ctgatgctga agcccgcctc
      721 cttcgctgcg cgtgccagca ccgacgagac atcgtggtcc tgcatctcga tcacccgacc
      781 acactgccgg cagatcagga actgcgcggc atgcgtgtgg atctcgccct caccctcatc
      841 atgatccgcc gtgcacccag caatacatcc gacgaacgcc gacagccgtt cgacccgatg
      901 gatcagtccc tgatccagca ggaaatccag cgcccggtac accgtcggcg gtgccgcccc
      961 acgtcgcgtc tgccgcaacc ggtccagcaa ttcataggcg cccgtgggcg ccgccgcgtc
     1021 gaggaccagc cccaacacat gccggcgcaa ttcagtcagc ctggcgccgc gatgctcaca
     1081 gatagcttct gcccgatcga gcaggttttc ggttcgccgt gagaactcgc ccgccattgt
     1141 tccttgccac tcggttgcgc aggatttgtt atagtattac ataacatgcg ccatggcaaa
     1201 acggctcctg cacctcatcc tggtctgtct gttcgctcgc tacgcggcag cacagcccct
     1261 ccccatcgtc gcggccgaaa acttctatgg cgatgtcgcg cgccagatcg ccggctccga
     1321 tgctcaggtc agcagtattc tcacgaaccc cgaccaggac ccgcacctct tcgaggctcg
     1381 cccctcagtt gctcgccagc ttgcgaccgc agccatcgtt atctgcaacg gcgccgacta
     1441 tgacccctgg atgaccaggc ttctcgccgc cacgcacact cctgatcgcc aggtcatcgt
     1501 cgttgccgac atcgtacaca ggaagccggg cgacaatccc cacctttggt acgatcctcc
     1561 gacaatgccc gcgtacgcca gcgcgctggc cacggctctc atacagcgtg atccgcccca
     1621 caaggcagag tacgaactgc ggctgcaaac cttcctgacc tcgctccaac cgctgcaacg
     1681 aaaaatcgcc gagatgcatg cccggttgaa cggcactccg gtcaccgcca ccgaaccggt
     1741 gttcagctac atggcaaccg cgctcggctt aaccatgcgc aaccaacgct tccagctcgc
     1801 tgtaatgaac gacaccgaac cccgcgcctc cgacatcgcc gcgatcgacg acgacttgcg
     1861 cgaacaccgc gtccacatcc tgttctacaa cagccaggcc accgacacgg cagcgcagcg
     1921 gctggtcgcc atcgcccacc aatccggcat ccctgtcgtc ggtgtcaccg agacagagcc
     1981 acctgacaag acctatcagc aatggatgac tgaccagctc gacgccgttg cccaggcact
     2041 ggcgaaccac ccgtcgtgaa cgccgccgaa ttctccggga cgacgctggc attcggcgat
     2101 cgcaccgtgc tcgcgaacgt cgatctcgcc atccgcgacg acgaattcat cggcgtgctc
     2161 ggcgctaacg gggcggggaa gaccacgctg atgcgggcga tccttggcct tgtcccggtg
     2221 cagcgcggcg ctatccgcgt gctcggcgca cccgtcgcgc gcggcaatcc ggccgtcggc
     2281 tacatgccgc agacccgagc cgcgctcgat catgtgcacc tcaccggctg ggacttcgtc
     2341 gcgagcggtt cggccggcca tcgctggggc cttccactgc tcaatcgtac catgcgccgt
     2401 gacgtggaat gggccctgga acgggtcgcg gcgaaagacc tcgcccgccg ccctctcacc
     2461 gagacctccg gcggcgagcg ccagcgcctc ctgctggccc aggccctgct cggccgtccc
     2521 cgcctcctgt tgctggacga accgctgatc agcctcgacc ctcatcacca gaccgcggtc
     2581 gtcgaactca cccgtgccct ccagcaggaa ctcggcatcg ccgtcctgtt cagtgcgcac
     2641 gagttgaacc cgctgctcgg cgccctcgat cgtgtcctct atctcggcag aagtcatgcc
     2701 gcgctcggca ccgtggacga agtgatcacc ggccccgttc tctcccgcct ctacggctcc
     2761 gacatcgacg tggtccgcgt caacggccgc attttcgtca tgtcaggcgg ccacgacgtc
     2821 gaacgcgacg cgcatcagca cgatcatcat gttcacgtat gacttcatgc agaacgcgtt
     2881 cgccgccgcc ggcatcgtgt cggtggtcag cgggctgatc ggcttcttcc ttgtcctgcg
     2941 cgcacagacc ttcgccggcc acgcgctctc ccatgtcggc ttcgccggcg ccacgggcgc
     3001 tgtcctgttc ggcctgccgc cgctgtgggg cctgatcgct ctgacattgg ccgccggtgt
     3061 cggaatggga ttgctgggcg aacagttata ccaacgcgat gtcgccatag gcatcgtgct
     3121 ggcaatgtca ttggggctcg gccttctgtt cctgaacttc ttcaccgcct acgcgacgca
     3181 ggccaccgct ctgctgtttg gcaacatcct gggcatcgac gatacaaccg tatggacgct
     3241 gcttgcgctc ggcgtcgctg cgatcgcggc gctcgccgcg atcgcgcgac ctctgctgtt
     3301 tgccagcctc caacccgagc tggctgaggc gaagggcgtc tctctgcgtc tcgtctcggt
     3361 cctcttcctc gcgatcaccg cgctcggtgt tgccgaatgc gcgcagatcg tcggcgtcct
     3421 tttggtgttc gcactgatgg tcgggccgcc cgctgctgcc caacgcctca ccgcgcactt
     3481 ctggccaggc atcatgctgt cggcgctgct cgctttggcc gaagcgtggc tcggtctgat
     3541 gctcgcctac gccaccgatt ggcctgccag cttctggatc accgcactca gcggcgcggt
     3601 ctatctcttg gccaccctgg ggatacgcag ccttcgatga ccctgtgacg cttcaggata
     3661 tctgcggcga ggatcgacat ttaatacgga gatggcggag gaccacggag aaccactgga
     3721 gttcgagcgg ctccgactcc tgtggtcctc cgccttctcc gtgttaaaca ttgcggcgcc
     3781 gtcctcatgc tccggccgcg cgtcgtgcag atccgatagc cctgccaccc atggcagcca
     3841 ccttgcctgc ccgcccgacc ccgactactg ctgtcgcgcg atgagatcgg tcatccgttt
     3901 cactctgatg tggctggcag cctggtgcca ggtgctggtg gtcgccacga tgcccctcgc
     3961 accgctcgcc atggcagcgg atccgctcgg cgaaatcccg atctgccacg cccccaaccc
     4021 agcagatggc cagcagaccc catcgcctga acacggccag cacgattgtg cactctgcgt
     4081 gatgtgctgc caggtgcatg catcgtcggc agcattgctg tcaccgacgc catccctgcc
     4141 cgagcgccaa accatcgcgg ccattcgtca ccacacggcg cagccacgcg ccccgcccgt
     4201 ccgcattgct gtcgccgcgc aaccacgcgg accaccctcc ccgacctgag cccgaccgat
     4261 cgccgccggc gcttgtcgct catgtcacag gagttcccac gtgccttctc ccagatgtct
     4321 ggccgcgctc accgccgcca gtttgttgcc ggtctcgagc ggatgggcgc acgggttcgc
     4381 cggcccccat atgttcatca gcacgctact gatagacgac cccaacgtcg ccgacgaagc
     4441 cagcctgccc accttcttct atctcccgca gccgaccgat ggcggtaccg cgccaaagct
     4501 gtacaacgtc agcttcgagt tcgataagcg gatcaccgag aacttcggct tctccatcag
     4561 cgacggctac aactggctac gcataccggg tgtcggcacc gccagcggct ggcagaacct
     4621 tgaagccacg ctgaaataca aggcctacgt gaacgacgag cacgaattca tgctctcgct
     4681 cggcgtctca cgcgagttcg cccgcaccgg agcaaccggt gccaacgggg caacgctcga
     4741 caacgacgac agcggctcca ccacgccaac aatctactgg ggcaagggct tcggcgacat
     4801 tcctgtgccg ctcctccgcc cgctcgcgct gaccggaacg ctcagctaca cgatcgccga
     4861 caacaaattg aaaatcacag gcaccgatcc cgacagcggc aatctgctgt tcaacaacgg
     4921 catatcgaac acgtggtcgg gcggcctgtc gctccagtac agcttccgct acctgcaaac
     4981 ccaggtgaag gactacggct ggcccgaatt tgtcaacaag ctgaccccgg tggtcgaagt
     5041 cgcctggtca tcgccggcga gcaagccgaa ccaggacagt acgcagtacc tcattggtgt
     5101 tggcctcaac tacaccgcca acagctacgc ggtgggtgtc gaagcattga tccccggcaa
     5161 cagacagacc ggctcgcacg ccggcgtcat tgcgcagttc catctgtatt tcgacgatct
     5221 gttccccaac agcctcggca aaccgatcgt ggggtggttc caatgatccg cgctgcgccc
     5281 tggctgtcgc tggcgatgct gctgatcgcg tcgccagcct tcgcgcacgc cctcctcgaa
     5341 cgcgcggaac cgcccgtcgg cagcgagatc accgccccgc cgcaccaggt cacgttgcgc
     5401 ttcaccgaag gtgtcgaacc agtgttcagc tcgatcgaaa tccatgacgc ccaaggtgcg
     5461 ctcgtgccta tcggcaagcc tcacacggtc ccaggcaacg atcgcacttt ggttgtcgac
     5521 ctgcccgacc tgcgcgccgg acgctacacg gtgacatggc acgtgacctc cgtggatacg
     5581 cacaagaccg agggcagttt ccagttcagt gtcatccact aacgcatgaa aggaacaaca
     5641 accatgcagc gaattctagc cacgctcctg ttgctgctcc ctatggcagc atccgccgaa
     5701 ccggcctcga tcaaggtcga gcaggtctgg tcgcgcgccg ccgtggttgg gcatgaaggc
     5761 gtggcctaca tgatcatcac caatgccggc cccaccgacg cgctgaccgc cgtctcaacc
     5821 ccaatcgccg ccactgcgga tctgcaccag accatcaatg acaacggcgt catgaaaatg
     5881 cgtcccgttc aatccctgcc gatcgacgcg ggcaagacgg tcacgctcgc gccgcgcggc
     5941 taccacatca tgctgacgaa cctgaagcag acactgaagg aaggcgacag cttccccatc
     6001 accctcactt tccagaaagc cggcaagatc acggcaacag caaaggtcga acaggccggc
     6061 gcgacctcgg cgccatctag cggcgcgtcc gatcacatga acatgccgat gtcaggcagc
     6121 aagccgtgat aggcgcgagc acggcaactc actccgacat cgaggccgcg gacagagctg
     6181 cgcggtcctg gcgttggcca gatccgcgcc cactgcgtct gggcagcgag gaacacaagc
     6241 aggcggcctg ccggatgttc cgcgagacgt tcaatccgta caagccggca gtcatcgact
     6301 ggccaaagct cgatccccac gcgctcgagc gtctgactcg cctgccgata tggaacatcg
     6361 cggtacagac cgagggcaac gcgcgcctcc gcatggccgc ctacgccagc agcctgctcg
     6421 atccggaatg gcgcgaggcc attggccaga acggctggga agaaggtcgc cacaaggacg
     6481 tcctcgccaa gctcgtcacg gcctacggca tccggctcga gcccgaaccc ccgtatctgg
     6541 aaccgcacga tacggaatgg gcctacctcg tcaccggctt cagcgagtgt atcgacagct
     6601 tcttcgcctt cggcctgttc gagcttgcaa ggcgctccgg cttcttcccc atcgaactcg
     6661 tcgacacatt cgagccggtg atccaggaag aggcccgcca catcctgctc ttcgccaact
     6721 ggctcgcctg gcaccggcgt cggatgccgt tatggaagcg tccttggttc gaggcgcgcg
     6781 tcgccgccgt gtgggctttc ctcgtctggg aacgcatcgg cctcgccaga accgtcgacg
     6841 gcggcggcac cgatagcaac ttcaccgtca atggcagccg ctcggtcagc gacgccgcga
     6901 tcaagcccgc cacgctgatg cgcatctgtc tcgacgagaa cgatcgccgc ttcgccggct
     6961 acgacccccg cctcctgcgc ccgactaccg tcccccggct tgtccgcttc gcccaacatt
     7021 tcctgcgcgg ctgatcacgt gtcgcgccca atggtaccca cctcctgtca ttgcgaggag
     7081 cggagcgacg aagcaatctc ggcaagatta cgcacagaat ccgttgccta ctgcgtgcgt
     7141 ccgatcgccc agctcagctc atgacccccc tggacatcgt cgtcgcctcg ctccgtggcc
     7201 tgcacctgat cgcactggtg tcagccttcg gcaccctgac cgccatcgcg atgacaccgg
     7261 cgacgacaaa agaatcggcg tatctacacc ggacactacg ccgcctcgtc cacctcagca
     7321 ctggctgcgc cctgatcacc ggcatcgcct ggctcaccac cgaatccgct gcgatcgccg
     7381 gcaccgacac catcgccaca accctgagcg ccattccgat cgttgccttc cagacccagt
     7441 atggccggtg gttcaccgcc cgctgcgtcc ttctgctgat cctgctgatc acccccttgg
     7501 cccgccccac cggcctcatc gccgcgctga tcctcgcggg tggcgccctc accatccagc
     7561 ccatggtcgg ccacgccggc gccatcggcg gtgccgtggg cacggagctg atcgtctccg
     7621 aggcactcca cctgttagcg gccggcgcct ggctcggcgg cctccttccc ctgttcctcg
     7681 ccgtatccat cctcccgcac gaggccgccg ccaccgcctg ccgcaacttc acacctgtcg
     7741 gtctctccgc cgtgttgatc ctggccggca ccgccatcgt gcagatcacc gaactgatgg
     7801 ggggcctccc tggcctgttc ggcaccacct acggccatct cgcgttggtt aagatcgcca
     7861 tcttcctcgt cttgctgatg ctggccgcgc taaaccgcct ggtcttcacc gagcatctcg
     7921 cgcacagcac ggccggcgtc acacaacgcc acatgcgcct ctcaatcgcc ctcgaaatgt
     7981 tgctcggcgc ttccgtcgtc gtcgccgcca gcttcctcgc cacactgacc cctggcacac
     8041 acgagcagcc tgtctggcca ttcccatggc gccccagcct gttcgccttc caggacccgc
     8101 tgctgcggcc agaaatctcc actgccgcca tcgcaaccgc catcgctctc gcgctgatcg
     8161 tcttcgctgt cgtgtggcgc cgggctcgct gggtcgcgct cgccctggcc gtcgtcatcc
     8221 tggccttcgc gctcccccac atcgacctgc tcttcatcga cgcctatccg accagcttct
     8281 tcacctcgcc caccgagttc gccgccacag cgatcgtgca tggtgccagg ctcttcgctg
     8341 cgaactgcgt cacctgccat ggcgccgacg cgaccggcga cggccccaac gccaaatccc
     8401 tcccgatccc gccagccgat ctcacagccg aacatttctg ggcccactcc gacggcgagc
     8461 tctactggta catcgcccac ggcttcgaag cccccggcgg cggcaccgcg atgcccggct
     8521 tcagtgccgc cctatccacc gaggcgatct gggatctcat cgactatctg cgggccaaca
     8581 acggtggcgc cagcatgcgc gccaccggca cctggtccca cccgctgcca gtgcctcagt
     8641 tcgatttccg ctgcgccgac aaacgcacgc tcgacctcga cgacatgcgc ggcaaaccca
     8701 tgcgtatcat tgccgcctcg gacgatgagc caatcgtccc gctgcccgac gtcaccacga
     8761 tctccgtcgt ccgcagccat gccgccgcgc cagccgcggg cggctgcgtc accagcgaac
     8821 cggaaacctg gacggcattt gccatcctgc tcggccaatc acccgccaca ctcgacggct
     8881 ggcagatcct tgtggatcag aacgcctggc tccgcgccgc ctggcatccc ggccagcctg
     8941 gcgactggac caatccggag accctggtca ggcgtatcca cgacatcgca agccatccgc
     9001 tcgacgtaat cctccccggc gcccacgtgc acatccactg accgcgcctt gccgcggttc
     9061 attacgcatg ctgcattgat gggaccagtg gaggacaggc caacttgagt ggggcactgc
     9121 cgcgcttact cgtcccccgg ctgcccttct tc
//
LOCUS       DATGEM010000462         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1271193, whole genome shotgun sequence.
ACCESSION   DATGEM010000462 DATGEM010000000
VERSION     DATGEM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271193"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1076)
                     /locus_tag="VKQ27_14615"
     CDS             complement(111..1076)
                     /locus_tag="VKQ27_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLY90211.1"
                     /translation="MTKPLLGVVDLHMHFPMRNAWGRRNGSLRAVDGVTLAVARGEIL
                     GVVGESGCGKTTLGKTIAGIHRASGGEILFEGHEIGALSPSDGRALRRTLQYCYQDPG
                     ASLDPHWKIGRALEEPLVIHTDLPADERRARVRDVLAAVGLPETHLDLYPHEISGGQQ
                     RRVGLARILMLRPTLVVLDEPTSGLDVSVQATVLRLFLELREQFKLTYLFISHDLSVV
                     RLISQRIAVMYLGKVVEIGATDAVFTAPRHPYTQSLLAAVPVVGGRRVTDDFWLEGEP
                     PNPGDLPSGCRFRTRCPRAEARCAAEEPALRSVADGVGVACHFAS"
     gene            complement(1073..2062)
                     /locus_tag="VKQ27_14620"
     CDS             complement(1073..2062)
                     /locus_tag="VKQ27_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90212.1"
                     /translation="MSAELLSVRELSVSFVTEAGMARVLDGVNLSLAAGEIMGLVGES
                     GCGKTTLSRTILGALPPNASIDGGALSFADHDLLRSSRVVRGRQITFVPQDPFSSFSP
                     LFTIGDQIMELMRWRSPDPRSARARRGDDRERVLNMLRAVQLPDPERLLRKYPHEVSG
                     GQRQRVMIAMALLPEPKLVIADEPTTALDVTIQAQILKLLRNLATERGVSVLFATHDL
                     GTAWEICDRVTVMYAGQDLETAPIASFFRAPMVPYTRSLLDSLPSGGSALRGIPGDVP
                     NLVTPPAGCRFHPRCPNADAVCRGEHPARREPEPGHQVRCHHPHLQAAGVVAA"
     gene            complement(2052..2894)
                     /locus_tag="VKQ27_14625"
     CDS             complement(2052..2894)
                     /locus_tag="VKQ27_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90213.1"
                     /translation="MMVFRRLLRDRSALLGLIILATLIVVAIFAYPLATFPQDVTSYD
                     PPHRLLPPSVTYWFGTDRMGGDIYSRLLFGARLTILIAVVATSVSVLIGVPVGLLAGY
                     YNNRFSDLLMRVSDIFLAVPQIVLAIAIAQTLGPAIHNVILALSITYWPFWARLVYAE
                     TRSVKNEVFIEAAIALGASPLRVMVLHVLPSIASSVIVRTTIGMGGTILVAAALGFLG
                     LGPPPPTPEWGRMIAESRGFLPQAWWYAAAPGMAILLVVMGFNLLGDGLRDVLDPRMR
                     RDVR"
BASE COUNT          658 a         1008 c          905 g          480 t
ORIGIN      
        1 ccaggaaaac cgcggtcatc gaacctcctg acgggcttta tctgcggatc gaagcaagtg
       61 aggctgcgga aatactgtca ctgtccgcag gcgcacggcc ccaggtctga tcaggacgcg
      121 aagtgacagg cgacgccaac gccatccgcg actgaccgta atgccggttc ctcagccgcg
      181 caccgcgctt ccgcccgtgg gcaacgcgtg cggaaccggc acccgcttgg tagatcgccg
      241 ggattcggcg gctcgccttc cagccagaaa tcgtcggtga cacgacgtcc acccacgaca
      301 ggcaccgccg caagcagcga ctgtgtatac ggatgccggg gcgcggtgaa cacggcatcg
      361 gtggcaccga tctcaacgac tttgccgaga tacatcacgg cgatgcgctg agaaatcagg
      421 cgcacgaccg aaagatcgtg gctgatgaac agataggtca gcttgaattg ctcgcgcaat
      481 tccaggaaca gccgcagcac ggttgcctgc accgacacat ccagcccgga tgtcggttca
      541 tccagcacca ccagggtcgg acgcagcatc aggatccgcg ccaggcccac acgtcgctgt
      601 tgacccccgc tgatctcatg tggatacaga tcgagatgcg tctccggcag tccgaccgca
      661 gccagcacgt cacgcacgcg ggcgcgacgt tcgtcagctg gcaagtccgt gtggatgacc
      721 agcggctcct ccagggcacg gccaatcttc cagtgcggat ccagtgaagc acctggatcc
      781 tgataacagt attgcagagt gcgccgcagc gcccggccgt cggacggcga cagtgcgccg
      841 atctcgtggc cctcgaacag tatttcgccg ccgctggcac gatgaatgcc cgcaatcgtc
      901 ttgccaagcg tcgtcttgcc gcagccagat tcacccacga caccaagtat ttcgccgcga
      961 gccacggcca atgtcacgcc atccaccgca cgtagcgatc cgttgcgccg cccccatgca
     1021 ttgcgcatgg ggaagtgcat atgaaggtca acgaccccca gcagcggttt cgtcatgctg
     1081 ccacgacacc agcagcttgc agatgcggat ggtggcaacg gacctggtgt cccggctccg
     1141 gttcgcgcct tgcgggatgc tcaccgcggc agacagcgtc agcattcggg cagcgtggat
     1201 ggaaacggca tccggccggc ggcgtcacca gatttggcac atcacccgga atgccacgaa
     1261 gcgccgaacc gcccgagggc aggctgtcca gcaatgagcg tgtatacgga accattggcg
     1321 cccggaagaa gctcgcgatt ggcgcagttt ccaggtcctg gccggcatac atgaccgtga
     1381 cgcgatcgca gatctcccac gcggtgccca ggtcatgcgt agcaaacagc accgacacgc
     1441 cacgttccgt tgctagattc cgcagcagct tcaggatctg cgcctggatc gttacatcca
     1501 acgcagtcgt cggctcatcg gcgatcacca gcttcggctc cggcaacagc gccatcgcga
     1561 tcatcacccg ctgccgttgc ccgcctgata cttcgtgcgg atacttgcgg agcaggcgct
     1621 ccgggtccgg caattgcaca gcacgcagca tgttcagcac acgttcacga tcatcaccac
     1681 ggcgcgcacg ggcggaacgc gggtcaggtg acctccagcg catcagttcc atgatctgat
     1741 cgccgatggt gaacagcgga ctgaacgagc tgaacggatc ctgcggcaca aaggtgatct
     1801 gacgaccacg caccacgcgc gatgatcgca acaggtcgtg gtcggcaaag ctgagcgcgc
     1861 caccgtctat gctggcgttc ggcggcaagg cgccgaggat agtgcgcgac agtgttgtct
     1921 tgccacagcc ggattcaccg accaacccca tgatttcgcc tgctgccagc gacaggttca
     1981 ccccgtcgag cacgcgcgcc atgccagctt cagtcacgaa agacacggac agctcgcgca
     2041 ccgacagcag ctcagcggac atcgcgccgc atcctcggat cgagcacgtc ccgcaagcca
     2101 tcgcccagca aattgaagcc catcaccacc agcaggatcg ccatgccagg ggctgccgcg
     2161 taccaccatg cctgtggcag gaagccgcgg ctctccgcga tcatgcgacc ccattcgggc
     2221 gtcggtggcg gtggacccag gccaagaaat cccaaggcgg cagcgacaag aattgtgccc
     2281 cccataccaa tcgtggtgcg cacgatgacc gacgacgcga tcgacggtag aacgtgcagc
     2341 accatcacgc gcagcggcga cgcacccagg gcgatagccg cctcgatgaa cacctcattc
     2401 ttcacactgc gggtctcggc atagaccagg cgcgcccaga acggccaata cgtgatcgac
     2461 agcgccagaa ttacattgtg gatcgccgga ccgagcgtct gtgcgatggc gatggccagc
     2521 acgatctgcg gcaccgccag gaagatgtca gagacccgca tcagcagatc gctgaaccgg
     2581 ttgttataat atcctgcaag cagcccgacc ggcacgccga tgagcaccga gaccgatgtg
     2641 gcgacgaccg caatgaggat ggtcagccgt gcaccgaaca acagccggct atatatgtcc
     2701 ccgcccatac gatcggtacc gaaccagtag gtcacactgg gcggaagcag ccggtgcggc
     2761 gggtcatagc tggtcacgtc ctgcgggaaa gtggccagcg gataggcaaa tatcgccacc
     2821 acaatcagcg tggcaaggat gatgaggcca agaagggccg atcggtcccg tagcagcctg
     2881 cggaagacca tcatggatcg ttgcgagcaa acgcccgctc cgaacacgcc atggttgaag
     2941 ctgatccagc catcatcggc cgcgtggatc cagcactgcc tgcgccacgt ctaccagcag
     3001 gtttgtcatg atgaacacca cgccgatgaa cagcgtgaag ctcatgatgg c
//
LOCUS       DATGEM010000463         4055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1211665, whole genome shotgun sequence.
ACCESSION   DATGEM010000463 DATGEM010000000
VERSION     DATGEM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211665"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="VKQ27_14630"
     CDS             <1..2601
                     /locus_tag="VKQ27_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90214.1"
                     /translation="GASSLGAGLQAAAQKAAQAKAEVTALNSELKANAAAGASSGVSA
                     MTQGFQNLGNVIATARTAITGFLAAAGVERFAGAISGALKLGEEMEILAAKTRIPVTA
                     LSDLKYAADQQNVSFEVLQQALRGLAVTTEEAIVKPTSRAAAGYAQLGISTEFLRANQ
                     NNLMAILTRVADAYATGGTAAQQADNAQIIFLRGGRELIPVLQLGAAGLDAAAERARA
                     FGNELSPGMATKLAEAEKKIKDANAAWEGMKNVLAVDLSPAIATTALELADLFAGTNE
                     SKIILYEQYLKNNIERLKEAQDAATAAQGEFAKLWYEGEIVRYGADIDAIRAKLTALR
                     LENQPNLAKGGIVPLPGPSVDVSSLPGGGPGAKPGVPMANPWKDLADRSTQATALEKF
                     REGLAEMEASWNGTHVAMLQEAVNMWASEVNSAKLNAKEKVEAEKGYADAVRALRQAQ
                     RAETEAIAKSDGDTNIAISKLQISAEKDILAAQFQAHQINADQRRALLQNLVDQEYQI
                     NLAELQRELGLLENQPAEWERVYNQIKLLKAQNVQDLAALDRQYTADVKRENEEQVAS
                     WKSAVSEIDSAEGSLVSDILTKRQSMTTTLAQITDRMIASEITADLRYYTNKLLYNAL
                     GLADDQKSATGGILAHLFEENSKNAATATGAATRRDIGATEDSSFLTRIPQMIGRWLG
                     LETSKTAATTAGATQREAVFTAEQLAGQAASVAAGFSQIEIDAAVAAAGAFAATAQIP
                     YIGPELAPEAAAAAYAATIGWAAGLGGGIAGLDVGAWSVRQGGLAYVHTGETVTPAVP
                     GPNFNQGMAAALAGGGGEGDIHIHGPLVQAIDTQTGAKFLLNNMQTIARGIAREIRNA
                     NGHLSAAARA"
     gene            2598..3242
                     /locus_tag="VKQ27_14635"
     CDS             2598..3242
                     /locus_tag="VKQ27_14635"
                     /inference="COORDINATES: protein motif:HMM:NF020901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HLY90215.1"
                     /translation="MSTAVFPSALMVNLAFPIKRSTLFEGEKMDSISGKETAVSYSSI
                     PRYIWDYPIQVLRQGATPPGGAATPWTEEAVLRGFVEQRFGGFDSWLFPDQDDSSVTG
                     QNIGTGNGSNLNFQLQRTRGGATMPILAPNAVSAVYVNAVLQGPTIYTVNGWGTATPG
                     VIAFAPGHAPGGGLAVTADFTYYFPVRFVMNQQEFQRFMTGLYEVKSLQFRSIK"
     gene            3239..3481
                     /locus_tag="VKQ27_14640"
     CDS             3239..3481
                     /locus_tag="VKQ27_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90216.1"
                     /translation="MNLPLWSVRFLGSPYASARVAAPDRATACLIIAAETGVEISAMR
                     DDPRPIGQRTAPVGVVTACGRIETDHAGILALVKRP"
     gene            3478..>4055
                     /locus_tag="VKQ27_14645"
     CDS             3478..>4055
                     /locus_tag="VKQ27_14645"
                     /inference="COORDINATES: protein motif:HMM:NF021451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HLY90217.1"
                     /translation="MTKPHTPEMQALWDSRQWYECSLLTFAGGNLGANVLRYCSGDQD
                     VVGADGQTYSCGGIDTGPYFDSKSSKAKVHTKVGLQVDTLTLEILPLNAQLFGVPFAY
                     ALASQILDGAELTIDTAYMPTWGNTSVGTLRKFVGRVADIDFGRGVIGLTINSHLELF
                     NGSFPANVFSAACGLSLGDTGCGVLLDGGSVV"
BASE COUNT          837 a         1217 c         1299 g          702 t
ORIGIN      
        1 ggcgcgtcct ctctcggtgc gggactgcag gccgctgccc aaaaagcggc gcaggcgaag
       61 gccgaggtca ccgcacttaa ttccgaattg aaagccaatg ctgctgctgg cgcatcgtcc
      121 ggcgtcagcg caatgacgca gggctttcag aatctcggca acgtcatcgc gacggctcgg
      181 acggcgatta ccggctttct cgcggcggct ggtgtcgagc gatttgccgg cgcgatcagc
      241 ggtgcgctca aacttggcga ggaaatggaa atcctcgccg caaagacgcg cattccagtc
      301 accgcgctat ccgatctgaa atatgcggcc gatcagcaaa acgtcagttt tgaggtcctg
      361 cagcaggcgc tccgtgggct cgcggttacg accgaggaag cgatcgtaaa gcccacgagt
      421 cgcgccgccg ctggatatgc ccagcttggc atcagcacag agttccttcg cgccaatcag
      481 aataatttga tggcgatcct gacgcgcgtt gctgatgcct acgcgaccgg tggcacggca
      541 gcacagcagg ccgataatgc gcaaatcatt ttccttcgcg gtggccggga gctgataccg
      601 gttcttcaac taggtgctgc gggtctggac gcggcggcag agagggcgcg tgcgttcggg
      661 aacgaactca gccccggcat ggcgacgaag ctcgctgagg ccgagaagaa gatcaaggat
      721 gcgaacgcag catgggaggg catgaaaaat gtcctcgccg tcgacctgtc gcctgcgatt
      781 gcgacgaccg cattggagct ggccgatctg ttcgctggaa ccaatgaatc gaagatcatc
      841 ctttacgaac aatatctgaa gaacaacatc gagcggctga aagaagcgca ggatgccgca
      901 acggcagcac agggcgaatt tgcgaagctc tggtatgaag gtgagatcgt ccgctatggc
      961 gccgatatcg atgcgatccg cgcgaaactc accgcgctgc ggctcgagaa tcagccgaac
     1021 ctcgcgaaag gcggcatcgt gccgctgccg ggcccgtcgg tcgatgtctc ctcgcttccc
     1081 ggtggtggtc ctggagcgaa gcccggagtg ccgatggcga atccctggaa ggatctcgcc
     1141 gataggtcga cgcaagcaac tgccctcgaa aagttccgcg aaggcctcgc cgagatggag
     1201 gctagctgga acggcacgca cgtcgccatg ctccaagaag ccgtcaatat gtgggcgagc
     1261 gaggtcaaca gcgcgaagct caatgcgaaa gagaaggtcg aggccgagaa gggctatgcc
     1321 gatgctgtgc gagcgcttcg ccaggcgcag cgcgctgaga ccgaggcgat cgccaaatcg
     1381 gatggcgaca ccaacatcgc gatttcaaag ctccagatca gcgccgagaa agacattctc
     1441 gcggcgcaat tccaggctca tcagatcaat gccgatcaac gccgcgcact cctgcaaaat
     1501 ctcgtcgatc aggaatacca gatcaacctc gctgagcttc aacgcgagct tggtctgctg
     1561 gaaaaccagc ccgccgaatg ggagcgggtc tataaccaga tcaaactcct gaaggcgcag
     1621 aacgtccaag acctggccgc gttggatcgg cagtacaccg cggatgtgaa gcgggaaaac
     1681 gaggaacagg tcgcctcgtg gaaatccgcc gtcagcgaaa tcgacagcgc ggaaggttcg
     1741 ctcgtcagcg atatcctcac gaagcgccag agtatgacga cgacgctggc gcagatcacc
     1801 gaccgcatga tcgccagcga gatcacggcc gacctcagat attacacgaa caagctgctc
     1861 tataacgcac tcggccttgc ggacgatcag aagagcgcga ccggcggtat cctcgcgcac
     1921 ctgtttgagg agaatagcaa gaacgctgca acggccaccg gagcagctac gcgccgcgat
     1981 atcggggcga ccgaggacag cagctttctc acacgcattc cgcagatgat cggacggtgg
     2041 ctcggtctgg agacgagcaa gacagccgca acgaccgctg gcgcgaccca gcgtgaggcg
     2101 gtgttcacgg cggaacagct cgcggggcag gccgcctctg tggcggccgg attttctcag
     2161 atcgagattg atgcggccgt ggctgccgcc ggtgccttcg cggcgacggc gcagatcccg
     2221 tatatcggcc ccgagcttgc acccgaggcg gcggcagcag cctatgcggc gacgatagga
     2281 tgggccgcag gacttggcgg cggcatcgcc ggcctcgacg ttggcgcgtg gagcgtgcgg
     2341 cagggcgggc tcgcatacgt gcacactggt gaaaccgtga cgcccgcagt gccagggccc
     2401 aacttcaacc aggggatggc agcggcgctc gctggcggtg gcggagaggg agacatccac
     2461 attcacggcc cgctcgtgca agcgatcgat acgcagaccg gggcgaaatt cctgctcaac
     2521 aacatgcaga ctatagcccg cggcatcgct cgtgagatcc gtaacgccaa cggccatctg
     2581 agcgcggcgg cacgtgcatg agcacggcag ttttcccgag tgcgctgatg gtcaatctcg
     2641 cctttccgat caagcggtcg acgctgtttg agggcgagaa gatggactcg atcagcggca
     2701 aggaaacggc ggtctcctat tcgagcatac cgcgctacat ctgggactat ccgatccaag
     2761 tgttgcggca gggcgcgacg cctcctggcg gtgctgcaac accctggacc gaagaggcgg
     2821 tgctccgcgg cttcgtcgag cagaggttcg gtggcttcga tagctggctc ttcccagatc
     2881 aggacgatag cagcgtcacc ggccagaaca tcggcacggg caacggcagc aacctgaatt
     2941 tccagcttca gcggacgcgc ggcggcgcta ccatgccgat cctcgcgccc aatgcggtct
     3001 cggcggtata tgtcaacgcg gttttgcagg gaccgacgat ctacaccgtt aatggttggg
     3061 ggacagccac gcccggcgtc atcgccttcg cgcccggtca tgcgcctggc ggtggcctcg
     3121 cagtcacggc ggactttacg tattacttcc cggttcgctt cgtgatgaac cagcaggagt
     3181 tccagcggtt catgaccggg ctctatgagg tgaagtcgct ccagtttcgg tcgattaagt
     3241 gaatctgcca ctgtggagcg tgcgcttcct cggaagcccc tacgccagtg cccgcgtcgc
     3301 cgcacctgac cgtgcaaccg cctgcctcat catcgcggcc gagacgggcg tcgagatcag
     3361 cgccatgcgt gacgatccga gaccgatagg gcagcgaacc gcaccggtcg gcgtggtgac
     3421 cgcttgcggg cgcattgaga cggaccatgc gggaattctt gcgctggtga aacggccgtg
     3481 actaagccgc acacgccgga gatgcaggcg ctttgggatt cgcgccaatg gtatgaatgc
     3541 tcgctcctga cattcgctgg cggcaacctc ggcgctaatg tcctacgcta ctgctcaggc
     3601 gatcaggacg tggtgggggc agacggtcag acctactcct gcggcggcat cgataccggg
     3661 ccctacttcg acagcaaaag cagcaaggcc aaggttcaca cgaaggtcgg gcttcaggtc
     3721 gacacgctga cgctcgagat cttgccgttg aacgcgcagc tttttggtgt tcccttcgcc
     3781 tatgcgctcg cgagccagat cctggacggc gccgagctca cgatcgacac ggcctatatg
     3841 ccgacctggg gaaatacgtc ggtcggcacg ctgcggaagt tcgtcgggcg agtggcggat
     3901 atagacttcg gtcgcggtgt cattggcctg acgatcaata gccacctgga attgttcaac
     3961 ggcagcttcc ccgcgaacgt gttctcggcg gcttgtggat tgagcctcgg agataccggc
     4021 tgcggcgttc tgctcgatgg cggcagcgtc gtcag
//
LOCUS       DATGEM010000464         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_141174, whole genome shotgun sequence.
ACCESSION   DATGEM010000464 DATGEM010000000
VERSION     DATGEM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141174"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1270
                     /locus_tag="VKQ27_14650"
     CDS             128..1270
                     /locus_tag="VKQ27_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY90218.1"
                     /translation="MGPLAGIRIVELAGIGPSPMCAMLLADLGATVLRIDRRQPSELG
                     IKRPLKYDLLLRNRKSIALDLKDPRVVTLTLELVAKADALIEGFRPGVAERLGLGPEP
                     CLARNRKLVYGRMTGWGQNGPLSHTAGHDLGYIAITGVLNAIGRADSPPSIPLNLVGD
                     YAGGSLFLALGIVSALLEARSSGQGQVIDAAIVDGTAALATTFFGMHAAGMLQPHRGS
                     NFGDSGAPFYDVYECADGKWLSVGPLEAKFYAEFLRILQLDPASLGPQMDREHWPRAK
                     VRLSQVFRTKPRSVWCGIFADSDACVAPVLDWQEAPAHPHLAARNTFVSVDGIVQPAP
                     APRFSRTVPEPPTPPQPVTPSNTDAALASWVDGSCVDRWRREGLID"
     gene            1284..2483
                     /locus_tag="VKQ27_14655"
     CDS             1284..2483
                     /locus_tag="VKQ27_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY90219.1"
                     /translation="MDLALTSEEIAFRAEVREFFREALPADIRSKAVLGQRLSKAELK
                     RWTQILHAKGWATPAWSPEWGGTGWSAVQQYIFKEELHMAPAPEPLSFNVNMIGPVLI
                     AFGSDQQKSHFLPRIANLDYWFCQGFSEPGAGSDLASLRTQAVRDGDDYVVNGQKLWT
                     STAHHADWCFLLVRTDPAAKKQQGISYLLMDMKSPGITVRPIVTIDGNHETNEMFLEN
                     VRVPAANRVGEENKGWDYAKYLLGHERSGIARVGISKMRLRRARTLAQRIPMGSSTLA
                     QDPGFRERAAALEIELKALEITQMRVIAESARGAAGKADPKTSILKIKGSELQQAAAE
                     LLLGVAAYEAMEYDGDLLRGLRDGDDEVDWARTLAPNHYFARHVSIVGGSNEIQHNII
                     AKSILGL"
     gene            2489..3625
                     /locus_tag="VKQ27_14660"
     CDS             2489..3625
                     /locus_tag="VKQ27_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY90220.1"
                     /translation="MDFDLTPDQELLQQSIGRLLADTYAFDQRRQHLQSAAGWSRALW
                     QQFADMGLLGLPFAADDGGFGGGAVEIMLIMESLGRVLSLEPYFSTVILAGSCLRLGA
                     DAAQRTRWLPPLIEGRETFAFAHVERAARYDLAHVKTRARYEDGAWRIDGRKDFVIHG
                     DTADKLLVSARTAGGPRDADGISLFMIDAAALRRRGYPTQDGTRAADLVLDDVRVADR
                     DVLGQPGAAMVLIDRVVDTAIAALAAEAVGAMTRAHELTVDYMKVRKQFGTTIGSFQA
                     LQHRAVDMLVMIEQARSMAMYATMMSEQADAAKRRQAVGGSKAFIGRAGRFVGEQAIQ
                     LHGGIGVTEECAVGHYYRRLTMIDVLFGDSAHHLGQVAAAGELP"
     gene            complement(3695..>3912)
                     /locus_tag="VKQ27_14665"
     CDS             complement(3695..>3912)
                     /locus_tag="VKQ27_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLY90221.1"
                     /translation="LKGCQIVGVIWGSATAHDPDLEPRIQRELVAMLAQGRIRPHIGA
                     RFVLDQGVEALRMLAERRALGKIIVHC"
BASE COUNT          699 a         1261 c         1271 g          681 t
ORIGIN      
        1 agttatgtca cgggcgacct catcgtcatc gacggcggcc gctggatgca aggagtgggc
       61 ggtcccagta atcgcgccat gcaggattgg accgatgcgc aatgggacgc catgcgcaag
      121 cgctgacatg ggaccactcg ccggaatccg catcgtcgaa ctggcgggca tcggcccctc
      181 gccgatgtgc gccatgctgc tcgccgatct tggagcgacg gtgttgcgga tcgatcgcag
      241 gcagccgtcg gaactcggga tcaagcgtcc tctgaagtac gaccttctgc tgcgcaatcg
      301 caagtccatc gccttggatc tcaaggatcc gcgcgtcgtc accctcacgc tcgaactcgt
      361 cgccaaggcg gacgcactga tcgaaggctt ccggcccgga gtcgccgagc gcttgggtct
      421 cggtcccgag ccctgcctgg ctcgcaatcg caagctggtc tacggacgca tgaccggctg
      481 gggccagaac ggcccactct cgcacaccgc cgggcacgat ctcggttaca tcgccatcac
      541 cggggtcttg aacgccatcg gccgcgccga ttcacctccg tcgattccat tgaatctcgt
      601 cggcgattat gccggcggtt cgctgttcct cgcccttggc atcgtgagcg cattgctcga
      661 agcccgcagt tccggtcagg gtcaagtcat cgatgcggcg atcgtggacg gcaccgcggc
      721 cctggcgacg acgttcttcg ggatgcacgc cgccggcatg ctgcaaccgc accgcggcag
      781 caatttcgga gattccggtg cgccgttcta cgatgtctac gaatgtgccg acggcaagtg
      841 gctgtcggtg gggccgctcg aggcaaaatt ctatgcggaa ttcctgcgga ttctgcagct
      901 cgatcccgcc tcgctcggtc ctcagatgga ccgggagcat tggccgcgcg ccaaggtgag
      961 gctgtcgcag gtgtttcgca ccaagccccg gagcgtttgg tgcgggatct ttgcggacag
     1021 tgacgcctgc gtggcccccg tgctcgactg gcaggaagcg cccgcacatc cgcacctggc
     1081 ggcccgcaac acgttcgtga gcgtggacgg catcgtgcag cccgcacctg cgccgcgctt
     1141 cagccgcacg gttcccgaac cgccgacgcc gcctcagccg gtgacgcctt cgaataccga
     1201 tgcggccctc gcatcctggg tggatgggag ttgcgtcgac cgatggcgcc gcgaaggatt
     1261 gatcgattga ccgaggttct ctgatggatc tagctctgac ttccgaggaa atcgcgtttc
     1321 gcgccgaggt gcgcgagttc tttcgtgagg ccctgccggc cgatatccgc agcaaggcgg
     1381 tgctcggcca gcgtctgtcc aaggcggagt tgaagcgctg gacgcagatt ttgcatgcga
     1441 aaggatgggc gacgccggcc tggtctcccg aatggggtgg aaccggctgg agcgccgtcc
     1501 agcaatacat cttcaaggaa gaactccaca tggcgccggc gccggaaccg ctgtccttca
     1561 atgtcaacat gatcggaccg gtgctgatcg ccttcggaag cgaccagcag aaatcgcatt
     1621 tcctgccgag aattgccaac ctcgactact ggttctgcca ggggttctcc gaacccgggg
     1681 ccggctcgga cctggcctcg ctgcgcaccc aagccgtgcg cgacggagat gactacgtgg
     1741 tcaacggcca gaagttgtgg acgtccaccg cgcatcacgc cgactggtgt ttcttgctgg
     1801 tgcgaaccga cccggcggcc aagaaacaac aggggatttc ctacctgctg atggacatga
     1861 aatcgccggg tatcacggtg cggccgatcg tcaccatcga cggcaatcac gaaaccaatg
     1921 aaatgttctt ggagaacgtg cgggtcccgg ccgccaaccg cgtgggcgag gagaacaagg
     1981 gctgggacta cgcgaaatat ctcctcggcc acgaacgcag cggcattgcg cgggtgggca
     2041 tctccaagat gcggctgcgc cgcgccagga cgctcgcgca gcggatcccc atgggctcgt
     2101 ccaccctggc ccaggatccg ggcttccggg agcgcgccgc cgcgctcgag atcgaactca
     2161 aggccctcga gatcactcag atgcgggtga tcgcggaaag cgcccgtggc gccgcgggca
     2221 aggcggatcc aaaaacgtcg atactgaaga tcaagggttc ggagctgcag caggctgcgg
     2281 cggaattgct gcttggagtg gccgcctacg aagccatgga atacgacggt gatctcctgc
     2341 gtgggctgcg cgatggcgat gatgaggtgg attgggcccg cacgttggcg ccgaatcact
     2401 atttcgcccg gcacgtgtcg atcgtcggag gatcgaacga gatacagcac aacatcatcg
     2461 ccaaaagcat tttaggtctg taggcagcat ggacttcgac ctgacgccgg atcaagaact
     2521 gctgcagcaa agcatcggcc ggctgctggc ggatacttat gcattcgacc aacgccgcca
     2581 gcacttgcag tccgccgcgg ggtggagccg tgcactgtgg cagcagtttg ccgacatggg
     2641 cctcctcggc ctgcccttcg ccgccgacga cggcggtttt ggcgggggcg cggtggagat
     2701 catgctcatc atggaatcgc tcgggcgggt gttgtcgctg gagccgtact tctccaccgt
     2761 catcctggcg ggaagctgcc tccggctcgg agcggatgcc gcgcagcgca cccgttggct
     2821 gccgccactg atcgagggcc gcgagacctt cgccttcgcg cacgtggagc gcgctgcacg
     2881 ctacgacctc gcacacgtaa aaacccgggc gcgatatgaa gacggcgcct ggcgcattga
     2941 cggccgcaag gacttcgtga tccacggaga taccgccgac aagctcctcg tgtccgcgcg
     3001 caccgccggc gggcctcgcg atgccgacgg catatcccta ttcatgatcg atgccgccgc
     3061 gctccgccgg cgcggctacc ctactcagga cggcacgcgg gcggccgatc tcgttctcga
     3121 tgatgtgcgg gtcgcggacc gcgatgtgct cgggcagccg ggcgccgcga tggttctcat
     3181 cgaccgggtc gtcgataccg caatcgccgc gctggccgcc gaggcggtcg gcgccatgac
     3241 gcgggcgcac gaactcacgg tcgactacat gaaagttcgc aagcaattcg gcacgaccat
     3301 cggctctttt caagcccttc agcaccgcgc ggtcgacatg ctggtgatga tcgaacaggc
     3361 gcgcagcatg gcgatgtatg caacgatgat gtccgagcag gccgatgcgg ccaagcggcg
     3421 ccaggccgtt ggcgggtcga aggcattcat cggccgcgcg ggccgtttcg tgggcgagca
     3481 ggcaatccaa ttgcatggcg ggatcggcgt caccgaagag tgcgccgtcg ggcactacta
     3541 ccggcggctg acgatgattg atgtgctgtt cggcgacagc gcccaccacc tcggccaagt
     3601 cgccgcggcc ggggaattgc cctgaagagc cgcgctcagc agcgcacgat cattttaccg
     3661 ggcgaacggc aatacaccat gatctcgccg gcgctcagca gtgcacgatg attttgccga
     3721 gcgcccgccg ctcggccagc atccggagcg cttcgacgcc ctgatccaga acgaatcggg
     3781 ccccaatatg gggacggatg cggccctgcg ccagcatggc gaccagttcg cgctgaatcc
     3841 gcggctcgag gtccggatca tgggcggtcg ccgagcccca aatgactccg acgatctggc
     3901 agcccttcaa ca
//
LOCUS       DATGEM010000465         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1379976, whole genome shotgun sequence.
ACCESSION   DATGEM010000465 DATGEM010000000
VERSION     DATGEM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379976"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1211)
                     /gene="paaE"
                     /locus_tag="VKQ27_14670"
     CDS             complement(144..1211)
                     /gene="paaE"
                     /locus_tag="VKQ27_14670"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HLY90222.1"
                     /translation="MAPRFHTLTIGDCRRETADAVSLAFAVPDDLRDDYAYSPGQYLT
                     LRASIDGEDVRRSYSICSGLGDNELRVAIKRVEGGTFSTWANEHLHIGSPLDVMTPDG
                     RFGVPMEPASPRTLVAFAAGSGITPILSIAKTALKREAGHFFLFYGNRTTAGIMFREQ
                     LEDLKDRYLSRLSVFHVLSREQQDLPALNGHLDAEKIGLVMRSLVPAASVDQAFVCGP
                     QPMIEGLEKALADLGMARDRIHVERFTPGVGGRPPPKTVAADAKPKAIATIISEGSRF
                     DLPVAEGEAIIDAAIRAGHSLPYSCKGGMCCTCRAKLVEGRVEMAVNYSLEPWETDAG
                     YVLTCQAHPVTEHVVIDYDQV"
     gene            1257..1673
                     /locus_tag="VKQ27_14675"
     CDS             1257..1673
                     /locus_tag="VKQ27_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HLY90223.1"
                     /translation="MRQAMHRITVETRGQGLVDVTDAVRRWVVAQAIGTGLLTVWCRH
                     TSASLLVQENADPDVRADLVAFFRRVVPEGAGYRHQDEGPDDMPAHIKAALTLTQLSV
                     PVGDGDLVLGTWQAIYLFEHRAAPHRRELVLHLIGD"
     gene            complement(1678..1947)
                     /locus_tag="VKQ27_14680"
     CDS             complement(1678..1947)
                     /locus_tag="VKQ27_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HLY90224.1"
                     /translation="MALRAVEKPDTKTGGIAADRLRSLVDRIERLEEERKALGGDIRD
                     IYAEAKSAGFDVKVLRQLIRIRKQEPADVEEQETLLDVYRRALGM"
BASE COUNT          351 a          613 c          723 g          384 t
ORIGIN      
        1 agatcgggtc gagcaccgaa agatcggcct ggggcacgaa tttcagcacg ttggatgcgg
       61 ctccgcgcgc gacggtgggg gtggcgaggg cggcggcggt cgaggcgagg aaggtgcggc
      121 ggcgcattca gcaagctcct ggatcagacc tgatcgtaat cgatcaccac atgttccgtc
      181 accggatgag cctggcaggt cagcacgtag cccgcatcag tttcccatgg ttcgagcgag
      241 tagttcactg ccatttccac gcggccttcc accagcttcg cgcggcaggt gcaacacatg
      301 ccgcccttgc atgaataggg caggctgtgg ccggcacgga tggcggcgtc gatgatcgcc
      361 tcgccctcgg cgacggggag gtcgaagcgc gagccttcgc tgatgatcgt ggcgatggct
      421 ttcggcttcg catcggctgc tactgtcttg ggtggagggc ggccgccgac gccgggggtg
      481 aagcgttcga cgtggatacg gtcacgcgcc atgccgagat cggccagcgc cttttccagg
      541 ccttcgatca tcggttgcgg gccgcagacg aaggcctgat cgacgcttgc cgctggaacg
      601 agcgagcgca tcaccaggcc gattttttcg gcatcgagat ggccattcag cgctggcaaa
      661 tcctgttgct cgcgcgacag gacgtggaag acggagaggc gtgagagata gcgatccttc
      721 agatcctcga gctgctcgcg gaacatgatg ccggcggtcg ttcgattgcc gtagaacaga
      781 aagaagtggc cggcctcgcg cttcagcgct gtcttcgcga tcgacaggat gggcgtgatg
      841 ccggaaccgg cggcgaaggc tacgagcgtg cgcggcgagg ctggttccat cggaacaccg
      901 aagcggccat caggcgtcat cacgtcgagc gggctgccga tatgcaagtg ctcgttggcc
      961 caggtggaga aggtcccgcc ctctacgcgt ttgatcgcga cacggagttc gttgtcgccg
     1021 agaccggagc agatcgaata agagcgacgc acatcttcgc catcgatact ggcgcgcagg
     1081 gtcaggtact ggccgggcga gtaggcatag tcgtcgcgga gatcatcggg gactgcgaag
     1141 gcgagtgaca ccgcatccgc cgtttcgcga cggcaatcgc cgatggtcaa agtgtgaaag
     1201 cgcggcgcca taatggccgt cttgcgcgat cagccggcgc ggcacaagtc tggcgcatga
     1261 ggcaggcgat gcaccggata accgtggaga cgcgcgggca agggctggtt gacgttaccg
     1321 acgcggtgcg tcgctgggtc gtggcgcagg cgatcggcac ggggctgctg acggtgtggt
     1381 gccggcacac ctcggcgtcg ctgctggtgc aggagaatgc tgaccccgat gtgcgcgcag
     1441 atctggtggc gttcttccgg cgcgtggtgc cggaaggggc ggggtatcgg catcaagatg
     1501 agggcccgga tgacatgccg gcacatatca aggccgcgct gaccctgaca cagctttccg
     1561 tgccggtggg ggatggcgat ttggtgctgg gcacctggca ggcgatctat ctgttcgagc
     1621 accgggctgc gccacatcgg cgggaactgg tgcttcacct gatcggtgat tgagcgccta
     1681 catccccagt gcgcggcgat agacgtcgag caaggtttcc tgctcttcca cgtccgccgg
     1741 ttcctgtttg cggatgcgga tgagctgacg cagcaccttc acgtcgaaac cggcggactt
     1801 tgcttcggcg tagatgtcgc gaatgtcgcc gccgagcgcc ttgcgctcct cctcgagccg
     1861 ttcgatgcgg tccaccaggc tgcgcagccg gtccgcggcg atgccgccgg tcttggtgtc
     1921 aggcttttcg accgcccgca gcgccattct gggtccctcc gattccggca aggccggcga
     1981 ggtacccgag cgatgcccgc gcggtcaatg accgggattt gatttcgcga aagccgcctt
     2041 ctgatccgcc gacgcctctt tctgatactt c
//
LOCUS       DATGEM010000466         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_802310, whole genome shotgun sequence.
ACCESSION   DATGEM010000466 DATGEM010000000
VERSION     DATGEM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802310"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VKQ27_14685"
     CDS             <1..1319
                     /locus_tag="VKQ27_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLY90225.1"
                     /translation="LPGLCLEASFMQVLRLCALVVFSAASFSAYAQPAATPPSLATRS
                     GEPVAAVVNDDVITQRDVEQRTKLALLTSNLADTPEMRSRVQGSVVRRLVDDDLKIQA
                     ATKRKISVSADDISAQMSAIEQQNHLPAGGLAKLLTSKGIEAETLRQQIRADIAWSRL
                     VGQVLAQGVHISENAVATRLDAIRANLGKPEYRASEIYLDVESAKDEAQARDLAERLS
                     EQMHQGAPFEAIARQFNQSGSADGNLGWVSDGMLDDELLAALDRLQPNGVTPPIRTTD
                     GYHILMLQEKRKVGEGLGGGGTVDVMTIDLNSLLSAGPVERDMQMQRLREILAPAGSC
                     DDLTRLSKQIPSASLSIAEKLPQTQVPTKVAALIKDLPPGRISEPIDTPKGRRFFAVC
                     GRTAGNSDGLPSADDIRLRMEEEQLELLARRQMLDLHRNAIIDIRQ"
     gene            complement(1333..1584)
                     /locus_tag="VKQ27_14690"
     CDS             complement(1333..1584)
                     /locus_tag="VKQ27_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90226.1"
                     /translation="MDDPLSYTQRLKLGAIGVRRDCADQARRQAIQAALLNDLRLCEM
                     LHAEADQHEAAIIEAIKEMALDRPAIVSMPSQRVVHRVF"
     gene            complement(1916..2893)
                     /locus_tag="VKQ27_14695"
     CDS             complement(1916..2893)
                     /locus_tag="VKQ27_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLY90227.1"
                     /translation="METPTPNTPKKNATTAKSTASDQNAIELLKADHREVEKMFEQFS
                     NAKGRQQRKKLIKQIATALTAHTIIEEEIFYPACRENDVEEDDLDQAQVEHDSVKMLV
                     RELLEGSLDDDYYDAKVTVLSEYVKHHVKEEEEPEKGIFARIQKTKADLDELGRELKE
                     RKDELMEDEDRLTERPPRIRSFHLGGQASRSGGGSSRHFGGQQEGRDERGRFTSEDDE
                     GSRSGYRGGGRNEWGAVRPPESADRQGYSSGPSERGDYAYGSQRDYDDRGYQGSSDRD
                     WRERGSSEDEEQRGRQRYRSEAGEGHGGWYGDSRDHSEAARRGWRNRDH"
     gene            complement(2982..>3522)
                     /locus_tag="VKQ27_14700"
     CDS             complement(2982..>3522)
                     /locus_tag="VKQ27_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90228.1"
                     /translation="DNDRGDYGRSANRERDEYGRFESSGRSEGGRYSEGSQRYQGARH
                     SQGGRDSEDRGRGGWSGDSERHSEASRRGWQDSDHGDSGWYGDSRGHAEASRRGWENP
                     SHGDSGWYGDPEGHSEASRRGWQNSSHEGSGWYGDSEGHSRAASQRSGGGGRYEEDDR
                     YDRYERGSTRGRGGRGRYD"
BASE COUNT          597 a         1155 c         1082 g          688 t
ORIGIN      
        1 acttgccggg tttgtgtctg gaggcatcgt tcatgcaggt cctgcggttg tgtgcgttgg
       61 tcgtcttttc cgccgcctcg ttctctgcct acgcacaacc cgccgcgacg cccccttccc
      121 tcgcaacccg ctccggcgag cccgtcgccg ccgtcgtcaa cgacgacgtc atcacgcagc
      181 gcgatgtgga gcagagaacg aagctggcgc tgttgacctc caatctcgcc gacacgccgg
      241 agatgcgcag ccgcgttcag ggatcggtgg tacgccgcct ggtcgacgac gatttgaaaa
      301 tccaggcggc caccaagcgc aagatttccg tctccgccga tgacatctcc gctcagatga
      361 gcgccatcga gcaacagaac cacctgccgg ccggcggttt ggccaaattg ctgaccagca
      421 agggaatcga ggccgagacg ttaaggcagc agatccgcgc cgacatcgcc tggtccaggc
      481 tggtggggca agtcctggcg cagggcgtgc acatcagcga aaacgcggtg gcgacgcgtc
      541 tcgacgccat tcgggccaat ctcggcaagc ccgaataccg cgcctcggag atttacctgg
      601 acgtcgaaag cgcgaaggat gaagcgcagg cccgcgatct tgccgagcgg ctgagcgagc
      661 agatgcatca aggcgcccct ttcgaggcca tagcgcggca attcaaccag agcggctctg
      721 ccgacggcaa tctcggctgg gtgtcggacg ggatgctgga cgatgagttg ctggcggcgc
      781 tggaccgtct gcagcccaat ggcgtgacgc cgccgatccg caccacggac ggctatcaca
      841 tcctgatgct gcaggaaaaa cgcaaggtcg gcgaagggct gggtggcggc ggcaccgtcg
      901 atgtgatgac catcgatctg aacagcctgc tcagcgccgg tccggtggaa cgcgacatgc
      961 agatgcagcg cttgcgcgaa atcctggcgc ccgccggaag ctgcgacgat ctgacccgtc
     1021 tcagcaagca gattccctcc gcctcgctct cgattgccga aaagctgccc cagacccagg
     1081 ttcccaccaa ggtcgcggct ctcatcaagg atttgccgcc gggccggatc agcgagccga
     1141 tcgacacgcc gaagggacgc cgctttttcg ccgtctgcgg gcgaacggcc ggcaattccg
     1201 acgggctccc ctccgccgac gatatccgcc ttcgcatgga ggaagaacag ctggaactgc
     1261 tggcgcgccg ccagatgctc gatcttcacc gcaatgccat catcgacatc cggcagtaag
     1321 gttcgtggtc ggtcagaaga cgcggtgcac gacccgctgt gacggcatgc tgacgatcgc
     1381 aggccggtcg agcgccattt ccttgatcgc ctcgataatc gcggcttcgt gctgatcggc
     1441 ctccgcgtgc agcatctcgc aaaggcgcag gtcgttgagc aacgccgcct ggatggcttg
     1501 tctgcgcgcc tggtcggcgc agtcacggcg cacaccgatc gcgccaagct tcaggcgctg
     1561 cgtataacta agcgggtcat ccatagtcga tcccctgggt tgaggagctt cgtccagtga
     1621 aattctgcca ggatttttac atgagtgctt caggctgtcc tgtgaccgaa cagtggtgat
     1681 caatttcgcc aatattcgtt gcataattgc ttgccgacac gaaatcgaca tcaccgttta
     1741 gtcactggct caaggccggc aacagaccgg tcgccggctc gcgaaatcca tccgcgcggc
     1801 aagagcggcg gggcttgtcg ccccgccatg aatgatttag tgggccttgg aagaactgtc
     1861 gtgagccggc ccggcgcgtg gagacacgcc gggccggtta tcgttggacc gccgatcaat
     1921 gatcgcgatt gcgccatcct cggcgcgcgg cttccgaatg gtcacgcgaa tcgccgtacc
     1981 atccgccatg gccttcgccg gcttccgagc ggtaacgctg ccggccgcgc tgttcctcgt
     2041 cctccgagga gccgcgttcg cgccaatccc ggtccgagct gccctgatag cctcggtcgt
     2101 cgtaatcgcg ctgcgagccg taggcgtaat cgccgcgctc cgagggaccc gacgagtacc
     2161 cttggcgatc ggcggattcg ggagggcgca ccgctcccca ttcattccgc ccgccgccgc
     2221 ggtagccgct gcgggaacct tcgtcatctt ccgaagtgaa gcggccgcgc tcgtcgcgac
     2281 cttcttgctg tccgccgaaa tgccgggacg agccgccgcc cgatcgcgag gcctggcccc
     2341 cgagatggaa cgagcggata cgcggcggac gctctgtcag acgatcctcg tcttccataa
     2401 gctcgtcctt gcgctccttg agttcgcggc ccaattcgtc cagatcggct ttggttttct
     2461 ggatcctggc gaagatgccc ttttcgggtt cttcctcttc cttgacgtgg tgcttcacat
     2521 attcggagag caccgtgacc ttggcgtcgt aatagtcatc gtcgaggcta ccctcgagca
     2581 gttctcgcac cagcattttg acgctgtcgt gttcgacctg ggcttggtcc agatcgtctt
     2641 cctcgacgtc attttcgcgg caggcggggt agaatatctc ttcttcgatg atcgtgtggg
     2701 cggtgagcgc ggtggcgatc tgcttgatca gcttcttacg ctgttgccgg cctttagcgt
     2761 tcgagaactg ctcgaacatc ttctcgacct cccgatggtc ggccttcagc agttcgatcg
     2821 cgttctgatc gctggccgtg gatttggccg tcgtcgcatt tttttttggg gtgttcgggg
     2881 tgggcgtctc cattcggacc tccgactgat aagagcaggc gagttgtaat agtgagaaag
     2941 agccgccggg caagacccgc cggcggctct cccctcaagc gtcagtcgta gcggccacga
     3001 ccaccacggc cgcgggtcga tccgcgctca tagcgatcgt aacggtcgtc ttcctcatag
     3061 cggccgccgc cgccgctccg ctgagaggct gcgcgtgaat ggccttccga atcgccatac
     3121 cagccgctcc cctcgtggct ggaattctgc cagccgcgac gcgaggcctc ggagtgaccc
     3181 tccgggtcgc cgtaccagcc actgtcaccg tggctcgggt tttcccaacc gcggcgggaa
     3241 gcttcggcat ggccgcgcga atcgccgtac catccactgt cgccatgatc ggaatcctgc
     3301 cagccccggc gggacgcctc ggaatggcgt tcggaatcgc cggaccatcc gccgcggccg
     3361 cgatcctcgg aatcgcgacc accttgcgag tggcgcgcgc cctgatagcg ttggctaccc
     3421 tcggaatagc gaccaccttc ggagcgaccg ctactctcga agcggccata ttcgtcgcgc
     3481 tcgcgattgg ccgagcggcc atagtcgccg cgatcgttgt cg
//
LOCUS       DATGEM010000467         7223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_53936, whole genome shotgun sequence.
ACCESSION   DATGEM010000467 DATGEM010000000
VERSION     DATGEM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53936"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VKQ27_14705"
     CDS             <1..1493
                     /locus_tag="VKQ27_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY90229.1"
                     /translation="NFWIHVPMGFSQVYANPRVNWMYESEPEAQLNGRTLYQPRGKVL
                     GGTSSINGMVYMRGNAADYDEWRQRGCTGWDWDSVLPYFKKAEHQERGANEFHGAGGP
                     LRVSDQPFHSELAQRLVEAAIQAGLPPIEDFNDGRQEGAGYFQSTTGDSRRWSTATAY
                     LRPSRNRPNLVVRTNAHAARIEIENGRAVGVTYMNGGAPKTARARGEVIVSGGVFNSP
                     QLLQLSGIGPGALLQEKGIPVIRDTPAVGSYMQDHFYVRLAYRCTRPITMNELANSLP
                     RKAIALAQWVLFRSGPLSGNGVTAGAFARSDPRLERPDLQFNFSPWSYASRDARGAVA
                     HPFPGFSLSAVHLRPDSRGSVQIKSPDPMAAPSIRFNFLQTQSDLQALTAGMRLARKF
                     TQQPALAPYVAEELIPGPATNTDAEFEANIRQNANSNLHPVGTCRMGPDGDSVVDPRL
                     RVHGVAGLRVADAAIMPTVPAGNTNAPTIMIGEKAADMILEDARRA"
     gene            complement(1573..4641)
                     /locus_tag="VKQ27_14710"
     CDS             complement(1573..4641)
                     /locus_tag="VKQ27_14710"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HLY90230.1"
                     /translation="MRPWFLRLIFACLLSGCAQPPPSAYVGGTSVALQGNQGISLGQN
                     ASGESCNQLSSGASGSFDVFCGTWQQPAARIRADGPGDAASLMAVATSSPWRDGINER
                     FACNAPTTTTILQGDPAVVLQCTRKIGGWPQIAVVAVASGRLYQADGILPTLPLIERS
                     IGVQSGQISAPTVALPRSAADSLLASQLAAHAFRATDVGEYQRLMALGARANLAENFT
                     AAETAYRAALALQQHALGHDDPDTVNALMHLALQVSDQGRFAEADALFKQAATLAPRA
                     SDKAAVSRLQHYEALNALNQKQYQQALTLLEQADRGYQALVPRESLQASNAGVSLIAA
                     NSAAALVAVPDQQLMIDPTAQSALMGLIEVRRYQSIALRHLGRPEESAAAIASAETLA
                     RGNRIGVPLVTARLTRTAATAQDLLGESGAAASGLAASRIDFTQVVPQTRPVAETALL
                     QAGVAAKQGDMTGAVALCERGTQLLRQLRSGTEPTLLEPCLATYASEAARQPANRQHL
                     LAEMFETAELAQDSTTSREIDEAAARLAANTKDPKIAEAIRRRQDSADHLAELYRQRD
                     AIAAGALPGSVPPGTPRDPAALDKPITDAQAELADADAALQAAAPNYGQLVQQVVPAS
                     DVLAALRPGEALAAITLTDAGGWTFLLRDGAIDVAPVKGNEASITALVKHVRASIESP
                     NGELPPFDTKSAKAIYDATLGPLTEQLTGAKALVVAPTGALLSLPFGLLLTGPADPAN
                     LATAPWLIQQMTIAHVPSAANFVALRKAGTSRAAHPWFGFGGFHPVTLAQADASFPGA
                     SCADSAKLFASLPPLPYAQRELDAARALLGASPSDELLNTAFTADAVRKMPLMDYRIL
                     HFATHALLPAELRCQDQPAIVTSAPPNARSASGALLTASDIVGLNLDTNVIILSACNS
                     GGPGNSTSGESLSGLARAFFFAGARSMLVTHWSINDQTSAFLVADTLRRLAANEDGGL
                     AGDLRAAQLGLIGRAGKDLPAQLAHPFFWAPFALIGEGVNQFGGTHTAMR"
     gene            complement(4645..>7223)
                     /locus_tag="VKQ27_14715"
     CDS             complement(4645..>7223)
                     /locus_tag="VKQ27_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90231.1"
                     /translation="ISARASDNGNGGRITILSTKDTSMAGTINAQGGPSGGNGGFVEV
                     SSDLGFSLTGAVDVSASKGGNGTILLDPLDLTIGAIGTDDGLLGGTIEPQIAFNTAPV
                     TPSAQDAFISNTAVNNLIGNIDLQASQDLFVNAPITLTAPGTGLGQSLTLEAGRNLVV
                     NSAISTAGNIILTASAAAPPTPPPLTYPGGFSTAGALTLNAPVSTSNGGIQLKAGSGG
                     INVNADVSANFVSANSGGAITISAAITAAGVYLSRVSLTATGPITESPTGSIVTPLFD
                     ASTTGTGAGIVLPSTANNITESNGITAANGDVILVNSQDLTLAGLHSGNNLFFEVHKN
                     GGTLTLTDFDPASDGGLNPATLNATGRISLVADNQVLAFAPSDAGAIVAASVELAPFS
                     SIPVSVLGTTGLVIDQTLLSIIQTNGGTLTVGGFTDVPGGATTPAASASSVDIAGALD
                     LGALTNANTLRLDAIGDITESGGPLTVNNLTGSGGLWQLNTSSNAIGALGDIVASNFS
                     LTDTGNLAVNGNVAASVDVALDLTGLLTINGQLSAPVVVLDANDIAIPGSLAAGTLAA
                     LVGNTFNETGAMTVGTLLAVSNGAMDLTGNNQVSSILGVSAPSFTLNDNSDLVITSAI
                     SANNIRILDPNHQITLGDGAVIVTGGSARPAGPIQPSLEPSNGAPGAYLQAANFVQIG
                     TSFVLSPTAGPNTLQIATTGNAQFDPPLGLFGSGTWLILNLGNGTAGGNVFVNALDVT
                     YNTPGSARLFGTIAGIGGGPAAAAGFIQPGVNVNYTFNGCVIASAVCNILPPPPPTFQ
                     LTNSDITSALGGLYPFLPGMPGPLATLPRLVVVALPMLPAAAPQLTDSDVVPPNISYL
                     DY"
BASE COUNT         1334 a         2232 c         2327 g         1330 t
ORIGIN      
        1 gcaacttctg gatccacgtc ccgatgggct tctcccaagt ctatgccaat ccccgcgtca
       61 attggatgta tgagagcgag ccggaagcac aactcaacgg ccgtactctg tatcagccgc
      121 gcggcaaagt attgggcggc accagctcga tcaacggcat ggtgtatatg cgtggcaacg
      181 cagccgacta cgacgaatgg cgccagcgcg gctgcaccgg atgggattgg gacagtgtcc
      241 tgccctactt caaaaaggca gaacaccagg aacggggtgc gaatgaattc catggagccg
      301 gcggtccgtt acgtgtctcg gaccagccgt tccatagcga actcgcccag cgcctcgttg
      361 aggctgccat ccaggccggc ctgccaccaa tcgaggattt caacgatgga cgccaggaag
      421 gtgctggcta cttccagagc acgaccggtg atagccgccg ctggagcaca gcgactgcgt
      481 atctacgccc gtcgcgcaac cgccccaatc tggttgtgcg caccaatgcg catgccgcgc
      541 gcatcgaaat cgaaaatggc cgagccgtcg gggtgaccta catgaatggc ggcgcgccga
      601 agactgcgcg cgctcgcggt gaggtgatcg tctcgggcgg cgtgttcaac tcaccacagt
      661 tgctgcagct gtcgggcatc ggtcccggtg cgctgttgca agaaaaaggg attccggtga
      721 tccgcgacac gccagccgtc ggcagctaca tgcaggacca tttctatgtc cgtctcgcgt
      781 atcgctgcac gcgccccatc accatgaatg agctcgccaa cagtctgccg cgcaaggcga
      841 tcgcactggc ccagtgggtc ctgttccgtt ctggcccact ttcgggcaac ggcgtcactg
      901 ccggcgcctt tgcgcgcagc gatcctcgcc tggaacgtcc tgacctgcag ttcaatttct
      961 ctccgtggag ttatgcgtca cgcgatgcgc gcggtgcggt ggcgcatcca ttccctggct
     1021 tcagcctcag cgccgtgcat ctgcgcccag acagccgggg cagcgtgcag atcaagagcc
     1081 cggacccgat ggcggcgccg tcgattcgct tcaacttctt gcagacacaa tccgatctac
     1141 aggcgctgac cgccgggatg cggttggcgc gcaagttcac ccagcagcca gcgctcgcac
     1201 cctacgtcgc ggaagaactc attcctggcc cggccaccaa cacagacgcc gagttcgaag
     1261 cgaatatccg ccagaacgcc aactccaacc tgcatcctgt aggcacctgc aggatgggac
     1321 cggacggtga ctctgtcgtc gatccgcgac tgcgcgtgca cggcgtcgct ggcctgcgcg
     1381 tggccgacgc ggcgatcatg ccgacggtgc cggcaggcaa caccaacgcg ccaaccatca
     1441 tgatcggcga gaaagccgct gacatgatcc tggaagacgc gcggcgggca tgatcggatc
     1501 gttctgtgat tgcgagcgga gcgaagcaat cccgacgaga ttgcttcgct ccgctcgcaa
     1561 tctcagcgaa acctaacgca tggcggtgtg ggtgccgccg aactggttca caccctcacc
     1621 gatgagcgcg aacggcgccc agaagaacgg gtgtgcaagc tgcgccggca agtccttgcc
     1681 tgcgcggccg atcaacccca gctgcgccgc acgcagatcg cccgccaatc caccatcctc
     1741 gttggcagca agccggcgca acgtatcggc caccaggaag gcggacgtct ggtcgttgat
     1801 cgaccaatgc gtcaccagca tcgaacgggc acccgcgaag aagaacgcgc gtgccagccc
     1861 cgacaggctt tcgcccgagg tgctgttgcc agggccacca ctgttgcacg ctgacaggat
     1921 gatcacgttt gtatcgagat tcagcccgac aatgtcgctc gccgtcagca acgcgccgga
     1981 cgcgctgcgt gcgttgggtg gtgctgacgt cacgatggcc ggctggtcct ggcaccgaag
     2041 ttcggccggc agcagcgcat gcgtggcaaa gtgcaggatg cggtagtcca tcagcggcat
     2101 cttgcgtacc gcgtctgccg tgaaggcagt attgagcaac tcgtccgacg gtgatgcgcc
     2161 gagcagcgcg cgcgccgcat ccagctcgcg ttgggcgtag ggcagaggcg gcaatgatgc
     2221 gaacagcttt gcgctgtccg cgcacgatgc gccggggaag ctggcatcgg cctgcgccag
     2281 tgtcaccgga tggaagccgc cgaagccgaa ccagggatgc gctgctcgcg atgtgcccgc
     2341 cttgcgcagc gcgacaaagt tggcggcgga cggcacgtgc gcgattgtca tctgctggat
     2401 cagccacggt gccgtggcga gatttgccgg gtcggccggt ccagtcagca acaggccgaa
     2461 tggcagcgag agcaaggcgc cggttggcgc caccaccaac gctttcgcac cagtgagttg
     2521 ctcggtgagc ggtccgagcg tcgcgtcgta gatcgccttc gccgacttcg tatcgaatgg
     2581 cggcagttcg ccgttcggcg actcgatgct ggcgcggacg tgcttcacca gcgcggtgat
     2641 cgatgcctca ttgcccttta ccggtgcgac atcgatggcg ccgtcgcgca gcaggaaagt
     2701 ccagccgccg gcgtcggtca gggtgatggc cgccagcgcc tcgccaggtc tcagcgcggc
     2761 cagcacgtcg gacgcaggaa ccacctgctg caccaattgc ccatagttcg gcgccgccgc
     2821 ttgcaatgcg gcatcggcat cggccagctc ggcctgcgcg tcggtgatgg gcttgtcgag
     2881 tgcggcagga tcgcgaggcg ttcccggcgg aacactgccc ggcaaagcgc ctgcggcaat
     2941 cgcatcgcgc tggcgataaa gctcggccag atgatcggcc gaatcctgcc gccggcggat
     3001 cgcttcggcg atcttcgggt ctttcgtgtt cgctgccaga cgcgccgccg cttcatcgat
     3061 ctcgcgactg gtcgtcgagt cctgggcaag ttccgccgtt tcgaacatct cggcgagaag
     3121 atgctgtcgg ttcgcgggct ggcgtgcggc ttcactggca taagtggcaa ggcatggctc
     3181 gagcagcgtc ggctcggtgc cggaacgcag ctggcgcagc aattgcgtgc cacgttcgca
     3241 cagtgccacg gcaccagtca tatcgccttg tttggccgcg acgccggctt gcagcagcgc
     3301 ggtttcggca acgggacgtg tttgcggcac gacctgggtg aagtcgatac gcgatgccgc
     3361 cagaccactt gccgcggcgc cgctttcgcc caacagatcc tgtgctgtcg ccgcagtgcg
     3421 agtgaggcgt gcggtgacca gcggcacacc gatgcgattg cctcgcgcaa gtgtctctgc
     3481 cgacgcgata gccgcggcac tttcctcagg ccgcccaagg tgccgcagcg cgatcgattg
     3541 gtaacggcgc acctcgatca gccccatgag agccgattgc gccgtcggat cgatcatgag
     3601 ttgctggtcc ggcacagcca ccagcgcagc ggcactattc gcggcgatca ggctcacgcc
     3661 ggcgttggat gcctgcaggc tctcgcgtgg caccagcgcc tggtagcccc gatctgcttg
     3721 ctcgaggagc gtcaacgcct gctggtactg cttctggttc agcgcattca gcgcctcata
     3781 atgctgcagg cgtgagacag ctgccttgtc actggcccgc ggcgcaagcg tggcggcctg
     3841 cttgaacagc gcgtcggcct cagcgaagcg tccctgatcg gatacctgca atgccagatg
     3901 catcagcgcg ttcactgtgt ccggatcgtc atggccaagc gcgtgctgct gcaaggcgag
     3961 cgccgcgcgg taggccgttt ccgccgcggt gaagttttcg gccaggttgg cgcgagcccc
     4021 gagtgccatc agccgctgat actcgccgac gtcggtggcg cggaaagcat gtgcggccaa
     4081 ctgggatgca agcagcgagt cagccgcgga tcgtggcaac gcgacggtcg gtgcgctgat
     4141 ctgaccggac tgcacgccga tggagcgttc gatcaggggc aatgtcggca ggatgccgtc
     4201 cgcctgatac agccgaccgc tcgccacggc caccacggcg atctgtggcc aaccgccaat
     4261 tttccgcgtg cattgcagca ccactgccgg atcgccttgc aggatcgtgg tggtggtcgg
     4321 cgcattgcag gcaaagcgct cgttgatgcc gtcgcgccac ggactgctgg tggcgacggc
     4381 cataagagat gctgcatcgc ccggcccatc ggcgcgaatg cgcgcggctg gttgttgcca
     4441 ggttccgcag aatacgtcga acgagccgct cgcgccggac gacagttgat tgcagctttc
     4501 gccgctggcg ttctgcccga ggctgatgcc ctggttgccc tgcaatgcca ccgaggttcc
     4561 gccgacatag gctgatggcg gcggctgcgc gcagcctgac agcaggcagg cgaagatcag
     4621 gcgaaggaac catgggcgca ttgtctaata atccaggtag ctgatgttgg gtggcacaac
     4681 atcagagtcg gtcagctgcg gtgcggcagc gggcagcatt ggcaaggcga caaccacgag
     4741 gcgtggcagc gtcgctagtg gccccggcat accaggcagg aaaggataaa gcccgccaag
     4801 tgcagaggtg atgtcgctat tggtgagctg gaaagttggc ggaggtggtg gcaatatgtt
     4861 gcagaccgcc gatgcgatca cgcagccgtt gaatgtgtaa ttgacgttga cgcctggctg
     4921 gatgaagcca gccgcagccg ccggcccacc gccgatgcca gcaatggtgc cgaacagtct
     4981 cgcgcttccc ggcgtgttgt aggtcacgtc gagcgcgttg acaaagacgt tccctcccgc
     5041 cgtgccgttg ccgaggttaa ggatcagcca ggtgccgctg ccaaagagtc cgagcggcgg
     5101 atcgaactgc gcgttacctg tcgtggcgat ctgcagcgta ttgggccccg ccgttgggct
     5161 gagcacgaag cttgtgccga tctggacgaa gttggcggcc tgtaggtagg cgccaggcgc
     5221 tccgttggac ggttcgagcg aaggctgaat cgggccggcc ggccgggcac tgccgccggt
     5281 cacgatcacc gcgccatcgc ccagggtgat ctgatggttc ggatcaagga tcctgatgtt
     5341 gttggcgctg atggccgagg tgatcaccag atcgctgtta tcgttcagcg tgaagctcgg
     5401 ggcactgacc cccaggatcg agctcacctg attgttgccg gtgagatcca tggcgccgtt
     5461 tgaaaccgcc agaagggtgc cgacggtcat cgcgccggtt tcgttgaacg tgttgccaac
     5521 aagggcggcc aacgtgccgg ccgccagtga accggggatc gcaatatcat tcgcgtccag
     5581 cacgaccacg ggcgcactaa gctggccatt gatggtgaga agaccggtca ggtcgagcgc
     5641 aacatccacg ctcgccgcca cattgccatt gacggcaaga ttgcccgtgt cggtcaggct
     5701 gaaattgctg gcgacaatat cgccgagtgc gccgatggcg ttcgagcttg tgttcaactg
     5761 ccacaagccg ccgctgccgg tcaggttatt cacggtcaag ggaccgccag actccgttat
     5821 gtcgccaatt gcgtccagac gcagtgtatt ggcgtttgtc aatgccccca aatcgagcgc
     5881 gccggcaatg tccacggagc ttgcggacgc cgcaggtgta gtggcgccac ccgggacatc
     5941 ggtgaagccg cccactgtca gcgtgccgcc gttcgtctgg atgatcgaga gcaaggtctg
     6001 atcgatcacg aggccggttg tgccaagcac actcacgggg atggaggaga aaggtgctag
     6061 ctccaccgat gcggccacga ttgcgccggc atcggacgga gcgaaagcga gaacttgatt
     6121 gtcggcaaca agcgagattc ggcccgttgc gtttagcgtc gccgggttaa ggccaccgtc
     6181 gctggccgga tcgaaatcgg tgagcgtcag ggttccacca ttcttgtgaa cctcgaagaa
     6241 caggttattt ccgctatgca ggccagccag tgtcagatcc tggctgttga ccaagatgac
     6301 atcgccattc gccgcagtaa tcccgttact ttccgtgatg ttgtttgctg tgcttgggag
     6361 cactatgcca gcaccagttc cagtcgtgct tgcgtcaaac aagggggtga cgatcgaacc
     6421 agtcggcgat tcggtaatcg gacctgtcgc ggtgagactg acgcggctga ggtatacgcc
     6481 cgcggcggtg atggcagcgg aaatggtgat tgccccaccg ctattcgccg acacaaagtt
     6541 ggccgaaacg tccgcattca cgttgatgcc gccggacccc gccttcaact ggatcccgcc
     6601 attgctcgtg ctaacggggg cgttcagagt caaagcgcct gcggtactaa agccgccagg
     6661 atatgtaagc ggagggggag tgggtggggc ggccgccgag gccgtcagta tgatgttgcc
     6721 tgccgtgctg atcgcgctgt tgacgacaag attccgcccc gcttcaagag tcaggctttg
     6781 tccgagcccg gttccgggcg cggttagcgt gattggtgcg ttgacaaaca ggtcctgcga
     6841 cgcctggaga tcgatatttc caatcaaatt attaacggcg gtatttgaaa taaacgcatc
     6901 ttgcgccgat ggggttacag gggcggtgtt aaacgctatt tgaggctcta tggtaccacc
     6961 taacaaacca tcgtctgttc cgatcgcacc tatagtcaga tccaaaggat caagcagtat
     7021 cgtgccattc ccccctttgg aggccgaaac atcaacggcg ccggtgagcg agaagcccaa
     7081 gtcgctcgat acttcaacga aaccgccatt accaccggac ggaccaccct gggcgttgat
     7141 cgtacccgcc atggatgtgt ctttggtgga caggatggtg attctcccgc cgttgccatt
     7201 gtcgctggca cgtgcgctga tct
//
LOCUS       DATGEM010000468         7313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1042277, whole genome shotgun sequence.
ACCESSION   DATGEM010000468 DATGEM010000000
VERSION     DATGEM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042277"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VKQ27_14720"
     CDS             <1..217
                     /locus_tag="VKQ27_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90232.1"
                     /translation="RSLLGQPTSRGGPAPGETWTYHSGSCEVHLYLFPNVDHGGMQVL
                     DHRVSGAGTGEGAQQACLRRLRDGQNS"
     gene            201..950
                     /locus_tag="VKQ27_14725"
     CDS             201..950
                     /locus_tag="VKQ27_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY90233.1"
                     /translation="MAKTVDSVSSPAQAAHVVLVDDDRAFLRVFAASLQAAGYQTECF
                     NDPRQALKALQRDVRPDACVLDWQMPDLDGLDLLKALRGRGFNMPVLFLTSHGQPIFE
                     EAALDAGAVDFVDKSRGPAIILHRLALALTRQPSQRNSVVTDSVVGALVLSQSSKRVR
                     WHGSHVPLSHSEFDVIELLARNAGRDVGYREIYDAARGSGFLAGQGEEGYRANVRAIV
                     KRIRRKFEQLDPAFTALQNYPGFGYRWHQDD"
     gene            943..2439
                     /locus_tag="VKQ27_14730"
     CDS             943..2439
                     /locus_tag="VKQ27_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY90234.1"
                     /translation="MTDPPGRGGLRWLRSIRVQAFALLVILIVLPALIFAVLGNAEAE
                     RRLLIRKAVAETGDTIAAGLVPVLHDLWPADLATLRSDLSRFAARDRSIKVLFRPKNA
                     VAADTFYFVATAPAISAEQTEAERQQLLRLGILPGLSQGCIARRLSGDSEASLLDNGA
                     QILTSVTSLEGVAGCWAVVIATSESRVLGAIEVRPYWERPEVRLAIGIYAVMALLIAA
                     IFSGVWFGLSRFRRLALSPTEPTGFARTTAIPELATLATAFDNMVHRMRSSADMLRQA
                     AEDNAHAFKGPIGIIRHAIALALRQTNSDKTREESMHSITTALDRLDGLVQSARYLDN
                     AAAELLQPEMVHVDLSALVRGFVRSYATMNTRRVRLDSHVTDGIAVAAQPENLEAILE
                     ALVDNALSFSPPDGSVTVKLEHDSESAILSVDDEGPGVAPERLERIFDRYYTHRPAAI
                     ACPRETSSHFGVGLWLARQNTIALGGTLTAINRDPHGLRMKVLLPLAG"
     gene            complement(2450..3892)
                     /locus_tag="VKQ27_14735"
     CDS             complement(2450..3892)
                     /locus_tag="VKQ27_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90235.1"
                     /translation="MPDSVGVYETDVPQRLDRLPWSSFHWLVIVALGTAWILDGLEVT
                     IVGSLSGALTESPVLHLSAGQVGLAASAYLVGAVAGALFFGWLTDRWGRKKLFSITVL
                     LYLCATIACGLSWNFASFALFRMLTGAGIGGEYAAVNATIQELIPARRRGFTDLVVNG
                     SFWLGAAVGAMGALVVLDPAVMPAEYGWRFAFIIGGLLGLIVLFLRQYLPESPRWLMT
                     HGDPQEAERVVAEIEQRVARDTGRALPPVPGKKLRLRTDVHEWFGPSARALLADYRRR
                     AVLGITLMAAQAFCYNAVFFTYALVLTRFYGVASGSIGWFMLPFAAGNFLGPLVLGKL
                     FDTLGRRFMIVLTYALSGILMAMTGWLFVLGVLDAVQQTLAWTVIFFVASAAASSAYL
                     TVGESFPLEMRAIAIALFYAFGTGVGGVAGPALFGALIDTGSRVSILWGYLLGGSLMV
                     VAAIVAAFLAVPAERRPLEEVAPPLSAAVG"
     gene            3948..4142
                     /locus_tag="VKQ27_14740"
     CDS             3948..4142
                     /locus_tag="VKQ27_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90236.1"
                     /translation="MDDFEAEIEATIARWHGIVAPNEPARRMAADLINTIRAFEALRG
                     TLVFEDEPSSFEAALLEAKE"
     gene            4148..5596
                     /locus_tag="VKQ27_14745"
     CDS             4148..5596
                     /locus_tag="VKQ27_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY90237.1"
                     /translation="MTNLAELSMVEAAEAVRGGRATSLELLDACWANMERLNPALNAT
                     IWLDREGAEASARAADRAVREKAWLGPLHGVPMMHKDMYYQAGKLSTCGSALRKDFRP
                     TVTATVIEKLAAAGAYTFGGLNMAEFAQNPTGHNRAFGDCHNPWNPPYITGGSSSASG
                     ASVAARFNYMAFGSDTGGSIRLPAAACGVTGIKPTQTRVSRYGVMPLSFSADNVGPLA
                     RTARDCARVMTLIAGRDPRDPTSSHEPVPDYEAALDGDIRGLRIGLPSTWFFDDVDQP
                     VLGALEHAVAVLVARGATVQQIALPMMDAVTTYGSIISRVEGATIHAHWMRERADAYG
                     IHISGRMYGGYALPATYYVEALSRRGPILKQFAKEVFSQVDVLITPTIRTCLPTLEAT
                     DIDHGPPGTEVRFMAVSANTRPFNYLGLPAVSVNCGFDPNGLPIGLQIAARPFAEVRV
                     LKVADAYQRDTDFHTQHPPILDSADASGVIVA"
     gene            5661..6656
                     /locus_tag="VKQ27_14750"
     CDS             5661..6656
                     /locus_tag="VKQ27_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HLY90238.1"
                     /translation="MKPKVAFIGTGGTIASLGVGPLDLQNYGATGNVMHADEIIARWP
                     ETALVADVLPVRYRNIPSTAIDFPDWRALVNVCEKIAAEHADIAGIVIGHGTATLEET
                     AYFLNLTLKVSVPVVLVGAQRPSSALSTDAGLNLVNAIRVAASPDARGLGVLVVLNDE
                     IQAARDVSKTSTLRLQTFRSRDFGVLGHADGDAIAFYRRPLRRCYPDTDFNVRALETL
                     PRVDVSYAYAGADGADVRAFIAAGAQGIVSAGFAPGFPSPGQAAALREAASQGITVAQ
                     STRAGSGRTFRGKRLAEAGILIADNLNPQKARILLALALTVTNDPDRIADMFRTY"
     gene            6668..7156
                     /locus_tag="VKQ27_14755"
     CDS             6668..7156
                     /locus_tag="VKQ27_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90239.1"
                     /translation="MIHHVSISARDPKHVADVLAELMGGRAYPFPGGVAKSFMAVSGD
                     ASGTLIEVYPEDVTLVPGTKDEQQCGCGHVEQPGYVPFHLLLSVPIDRAKVEEIGARE
                     AWRTKYYGRGAPGQKPFFYVIEFWVENRIMIEVATPDMVTEYTQTIRFDRLDAMFNRA
                     AA"
BASE COUNT         1360 a         2499 c         2202 g         1252 t
ORIGIN      
        1 acgatcgctg ctgggacaac ctacgtcgcg tggcgggccc gcccctggcg aaacgtggac
       61 ctatcactcc ggctcctgcg aggttcacct gtatctgttc cccaatgtgg accatggcgg
      121 aatgcaggtt cttgatcacc gggtcagcgg cgcaggcacc ggcgagggcg cccagcaggc
      181 gtgtctgaga cggttgcgcg atggccaaaa cagttgattc ggtcagttcc cccgcccagg
      241 cggcacatgt ggtactggtc gatgatgatc gggcgttcct tcgcgtgttt gcagcaagcc
      301 tgcaggcagc aggctaccag accgagtgtt tcaatgatcc caggcaggcg ctgaaagccc
      361 tccaacggga cgtccggccg gatgcatgtg tcctcgattg gcaaatgccg gacctcgatg
      421 gcctcgacct gctgaaggca ctgcgcggcc gcggtttcaa catgccggtg ctgttcctca
      481 catcgcacgg ccagcccatc ttcgaagagg cggcgcttga cgctggcgct gtagactttg
      541 tcgacaagtc gcgggggccg gcaatcatcc tgcaccggtt ggcgctcgct ctgacgcgcc
      601 aaccttcgca acgcaactca gtcgtcacag acagcgttgt cggcgcgctg gtgttgtcgc
      661 agtcttccaa acgcgtgcgc tggcatggca gccacgtgcc gctcagccac agcgaattcg
      721 acgtcattga attgctcgcc agaaacgcgg gtcgtgatgt cgggtatcgc gagatctacg
      781 atgcggcacg tggcagcgga tttctcgccg gtcaaggcga ggagggatac cgcgccaatg
      841 tgcgcgcaat cgtcaaacgg attcggcgca agttcgaaca actggatccg gctttcaccg
      901 cattgcagaa ctaccccggc tttggctacc gctggcacca ggatgactga ccctccgggc
      961 cggggcggcc tgcgctggct gcgctcgatc cgcgtccagg cctttgcgct gttggtgatc
     1021 ctgatcgtgt tgccggctct tattttcgcc gtgctgggca atgccgaagc cgagcgccgt
     1081 ctgctgatcc gcaaggcggt cgcagagaca ggagacacca tcgccgcggg ccttgttccg
     1141 gtcctgcacg atctatggcc ggcggacctc gccactttgc gaagcgacct gtcgcggttc
     1201 gctgcgcgcg atcgcagcat caaggttctg ttccggccga agaatgccgt tgcagccgac
     1261 acgttctatt tcgtcgcgac cgcacccgcg atctctgccg agcagacaga agccgagcgg
     1321 cagcagttgc tgcgcttagg cattctgcct ggactgtcac aaggttgcat tgcgcggcga
     1381 ctgtccggcg atagcgaggc atcgctgctg gacaatggcg cgcagatcct gacctccgtg
     1441 acctcgctcg aaggcgtggc gggatgctgg gccgtcgtga tcgcaacgag cgaaagccgg
     1501 gtcctgggag cgatcgaggt caggccttac tgggaacggc ccgaggttcg cctcgccatc
     1561 ggcatctacg ccgtgatggc gttattgatc gcggcgatct tttcgggtgt gtggtttggc
     1621 ctgtcgcgct ttcgccggct cgccttgtcg ccgacggagc caaccggctt cgcacgcacg
     1681 actgccatcc ccgaactggc gacgctcgcg accgcgttcg acaacatggt gcaccgcatg
     1741 cgttccagtg ccgacatgct gcgccaggcc gccgaggata atgcccatgc cttcaaagga
     1801 cctatcggca tcatccgcca tgcgatcgca ttggcactgc ggcagaccaa ttcggacaag
     1861 acgcgcgagg agtcgatgca tagcatcacc acagcgctcg accgattgga cgggctggta
     1921 cagtccgcgc gttatctcga caacgcggcc gccgaattgc tgcagcccga aatggttcat
     1981 gtcgatctct cagcgctcgt gcgtgggttc gtgcgtagct acgcgacgat gaacacgcgc
     2041 cgtgttcggc tcgactcgca tgtgaccgac gggattgcag tggccgcaca gcccgagaac
     2101 ctggaagcga tacttgaagc gctggttgac aacgcgctga gcttctcacc gcccgatgga
     2161 tccgtgacgg tgaagttgga gcatgactcg gaatcggcga tcctctctgt ggatgatgag
     2221 gggcctggcg ttgcgccaga gcgtctggaa cgcatattcg atcgctatta cacgcaccgg
     2281 ccggcagcca tcgcctgccc gcgtgaaacc tcgtcgcact tcggtgttgg cctttggctg
     2341 gcgagacaga acacgatcgc tctcggcggc acactcacgg cgatcaatcg cgatccgcac
     2401 ggtctgcgca tgaaggtcct gcttccgctg gctggatagc cgcgcaaatt cagcccaccg
     2461 cggcagataa tggtggcgca acctcctcca atggccgccg ctctgccggt accgccagaa
     2521 acgccgcgac gatggcggct accaccatca acgagccgcc cagcagatag ccccacagga
     2581 tggacacgcg cgatcccgta tcgatcagcg cgccgaacag ggccggcccg gccacaccgc
     2641 ccacgccggt gccgaaggca tagaacaacg cgatcgcgat cgcgcgcatc tccagcggga
     2701 agctctcacc taccgtcagg tacgccgagc tcgccgccgc cgaggcaacg aagaagatca
     2761 ccgtccaggc cagcgtctgc tgcacggcgt ccagcacgcc cagcacgaac agccacccgg
     2821 tcatcgccat caggatgccg gacagcgcat aggtcagcac gatcatgaag cgccgtccca
     2881 gcgtgtcgaa caacttgccc agcaccagcg gccccagaaa atttcccgcg gcgaacggca
     2941 gcatgaacca gccgatgctg cccgacgcaa caccgtagaa ccgcgtcagg accagtgcgt
     3001 aggtgaagaa cacggcgttg tagcagaacg cctgcgccgc catcagcgtg atgccaagga
     3061 ctgcgcggcg ccggtaatca gcaagcaacg cacgtgccga aggaccgaac cactcgtgca
     3121 cgtcggtgcg cagccgcagc ttctttccgg gcaccggcgg caaagcacgc cctgtatcgc
     3181 gggccacccg ctgctcgatc tcagccacca cacgttcagc ttcctgtgga tcgccgtgcg
     3241 tcatcagcca acgcgggctt tccggaaggt actggcgcag gaacagcacg atcagcccaa
     3301 gcaggccgcc aatgatgaac gcaaaccgcc agccatattc ggccggcatc accgccggat
     3361 cgagcaccac cagcgctccc atcgccccga ctgccgctcc cagccagaag ctgccgttga
     3421 cgacaagatc ggtgaaccct cgccgacgcg ccgggatcag ctcctggatg gtggcgttca
     3481 ccgccgcata ctcgccaccg atcccggccc cggtcagcat ccggaacaat gcaaacgagg
     3541 cgaaattcca cgacaaccca caggcgatcg tcgcgcagag gtacagcagc accgttatgc
     3601 tgaacagctt cttccgcccc caccgatccg tcagccaccc aaagaacagc gcccccgcga
     3661 ccgcacctac cagatacgcg ctggctgcca gtcccacctg cccggcgctc agatgcagca
     3721 ccgggctctc ggtcagcgcc cccgacagag acccgacaat ggtcacttcc agcccatcca
     3781 ggatccacgc cgttcccagt gcgacgatca ccagccagtg aaagctgctc catggcaacc
     3841 gatccagccg ctgcggcaca tcggtctcat acacccccac actgtccggc atgtcgcttg
     3901 cctcgtctcc ctggcatagt cccgctcaac gcacggggag accgctcatg gacgactttg
     3961 aagccgagat cgaggcaacg attgcgcgat ggcacggcat tgtggcgccg aatgagccgg
     4021 cacggcgcat ggcagccgac ctgatcaaca ccatccgcgc cttcgaggct ctgcgcggca
     4081 cgctggtgtt tgaggacgaa ccgtccagct tcgaagccgc cctgctcgaa gcgaaggagt
     4141 gaggcagatg accaatctgg ccgaactctc catggtcgaa gccgccgagg ctgtccgcgg
     4201 cggccgagcc acttcgctgg aactgctcga cgcctgctgg gccaatatgg agcgcctcaa
     4261 tccggcattg aatgcgacca tctggctcga ccgcgaaggc gcagaggcct cagcccgcgc
     4321 ggcagatcgc gcggtgcgcg agaaagcatg gctcggaccg ctgcacggcg tgccgatgat
     4381 gcacaaggac atgtactacc aggccggcaa gctctccacc tgcggttcgg cactccgcaa
     4441 ggatttccgt cccaccgtca cggcgaccgt gatcgaaaag ctggccgcgg ccggcgctta
     4501 tacgttcggc ggcctcaaca tggcggagtt cgcccagaac ccgaccggtc acaatcgcgc
     4561 gttcggcgac tgccataacc cgtggaaccc accttacatc accggcggct cgtcatcggc
     4621 atccggcgcg tcggtggcgg cacggttcaa ctacatggcg ttcggctccg ataccggcgg
     4681 ctcgatccgc ctgcccgctg cggcctgcgg cgtgaccggg atcaagccga cgcaaacgcg
     4741 cgtttcccgc tacggcgtga tgccgctgtc gttcagcgcg gacaatgtcg ggcccctcgc
     4801 ccgcaccgcg cgcgactgcg cgcgggtcat gacgctgatc gccggccgcg atccgcgcga
     4861 tcccaccagc tcgcacgagc cggtgccgga ctacgaagca gccctcgatg gcgacattcg
     4921 cggtctgcgc atcggccttc cctcgacctg gttcttcgat gacgtcgacc agccggtcct
     4981 gggtgcactc gaacacgctg tcgctgtgct ggttgcacgc ggcgcgacgg tgcagcagat
     5041 cgcgctgccg atgatggacg cggtgacgac ttatggcagc atcatctcgc gcgtcgaagg
     5101 cgcgacgatc cacgcgcatt ggatgcgcga acgcgccgac gcatacggca tccacatcag
     5161 cgggcggatg tacggcggct acgcgctccc ggccacctat tacgtcgaag ctctgagccg
     5221 gcgcggacct atcctgaagc aattcgcgaa ggaggttttc tcccaggtcg acgtactgat
     5281 cacaccaacc atccgcacct gtttgccgac actcgaagcg accgacatag atcatggtcc
     5341 acccggcact gaagtaaggt tcatggcagt ctcggcgaac acgcggccgt tcaactacct
     5401 cggccttcct gcggtcagcg taaactgtgg cttcgatccg aacggtctgc cgatcggctt
     5461 gcagatcgcc gcgcggccat tcgcagaggt gcgcgtgctg aaagtcgccg acgcctacca
     5521 gcgcgacact gacttccaca cgcagcaccc gccgatactg gacagcgcag acgcaagcgg
     5581 cgtgatcgtc gcatgacgcc ccaccctcgt catcgtcgcg ctcggcgcga ccacccacga
     5641 cttctgtgtg agccgaaacc atgaagccaa aggtcgcctt catcggcaca ggcggcacga
     5701 tcgcctcatt gggggttggc ccactcgacc tgcagaacta cggtgcgacc ggcaacgtga
     5761 tgcacgccga cgagatcatc gcgcgctggc cggaaaccgc gctggtcgcc gacgtgcttc
     5821 ccgtgcgcta ccgcaacatc cccagcactg cgatcgactt cccggactgg cgcgcgctgg
     5881 tgaatgtgtg tgaaaaaatc gccgcggagc atgccgatat cgctggtatc gtcatcggcc
     5941 acggcacggc gacactggag gaaactgcgt atttcctcaa tctgacgctg aaggtatccg
     6001 tccccgtggt gttggtcggc gcacaacggc cgtccagcgc tctatccacg gatgccgggc
     6061 tcaatctggt gaacgccatc cgcgtcgccg caagtcccga tgcgcgcggc ctgggcgtcc
     6121 tcgtcgtatt gaatgacgag atccaggcag cgcgcgacgt atccaagacc tccacattgc
     6181 gtctgcagac cttccgctcg cgtgacttcg gcgtgctcgg ccatgcagac ggcgatgcga
     6241 tcgccttcta tcgccgccca cttcgccgct gctatccaga caccgacttc aacgtgcgcg
     6301 cgctcgagac tttgcctcgc gtcgatgtgt cctatgccta cgcaggtgcg gatggcgccg
     6361 atgtgcgcgc tttcatcgcg gccggcgcac aaggcatcgt gtcggccggc tttgcgcctg
     6421 gttttccctc gccaggccag gccgccgccc tccgcgaggc cgcctcgcag ggcatcaccg
     6481 tcgcgcaatc gacacgcgcc ggctccggcc gtacattccg cggtaagcgc ctggccgagg
     6541 ccggtatcct gatcgccgac aacctcaatc cgcagaaggc acgcatcctg ctcgcgctgg
     6601 cgctcaccgt aaccaacgat ccggatcgca tcgccgatat gttccggacc tattgaggag
     6661 acatctgatg atccaccacg tctcgatttc cgcccgcgac ccgaagcacg tcgccgacgt
     6721 gctggcggaa ctgatgggcg gacgcgccta tccgtttcct ggcggcgtgg cgaagtcatt
     6781 catggccgtc agcggcgacg ccagcggcac gctgatcgag gtttatcccg aggacgtcac
     6841 gctcgttccc ggcaccaaag acgagcagca atgcggctgc ggccatgtcg agcagccggg
     6901 ctacgtgcca ttccacctgt tgctgtcagt gccgatcgac agggcgaagg tggaagagat
     6961 cggtgcacgc gaggcctggc gcaccaagta ttatggccgt ggcgcaccgg gtcagaagcc
     7021 gttcttctac gtgatcgagt tctgggtgga gaaccgcatc atgatcgagg tggcgacgcc
     7081 tgacatggtg accgaataca cgcagaccat ccgcttcgac cggctggacg cgatgttcaa
     7141 ccgcgccgct gcctagacac ctatgtccgc gccctccccg cgcccatgtc catcgcccca
     7201 tgtccacccg catgtcattg cgaggagcgg agcgacgaag caatccgagg gtaggctgac
     7261 agcactgcgc cgcgtccaac ccggccactg ctgctaacag tcgcaccttt gtc
//
LOCUS       DATGEM010000469         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_923179, whole genome shotgun sequence.
ACCESSION   DATGEM010000469 DATGEM010000000
VERSION     DATGEM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923179"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VKQ27_14760"
     CDS             <1..556
                     /locus_tag="VKQ27_14760"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLY90240.1"
                     /translation="GEHLPNREKHGKQLVAVWHSEVGQLNQFIHVWAYDNFEQRTTVR
                     AAAMKAGGWPPPIGEFLVEMKNEIFYPAPFSPPLEAREIGPLFEIRQYTLAPGSIPGL
                     IERWSEKIEGRQKFSPLVAGMYTEFGALNKWVHIWAYKDANERNSVRASAAATGQWPA
                     RNPPGVVIKQENAFVMPAAYSPIR"
     gene            682..1827
                     /locus_tag="VKQ27_14765"
     CDS             682..1827
                     /locus_tag="VKQ27_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLY90241.1"
                     /translation="MDQNLAEKLTAWRRHLHAHPELTLHEKETAAFVCGRLTELGVSF
                     VAGVGGHGVVATIQRGQSNRSVGLRADMDALPITETTGLGHASTNPGVMHACGHDGHT
                     TSLLGAAALLQQDTSWSGTVHLVFQPAEEGGGGAKSMIADGLFERFPMERIFGYHNWP
                     GLDAGAVAVHDSAVMASGSRIEFHVKGHSGHAALPHLTRDPMVASAHLLLALQSIVSR
                     NVDPLDSAVISVATMQAGTAANQVPGEAIMRGTMRTMRSETRDLVEQAIERVAAGVAQ
                     ACDVAIDVTMRRGNPVTVNSVAERDLAADAVVAAGLPLRRDIPPAMTGEDFAWYLQQR
                     PGAFVWIGNGPAENGRELHNSGYDYNDAILPAASGFLAGVARRALGS"
     gene            complement(1832..1999)
                     /locus_tag="VKQ27_14770"
     CDS             complement(1832..1999)
                     /locus_tag="VKQ27_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLY90242.1"
                     /translation="MKTAQTRLNDAGFEVDYLALVDGPSLRAISVRQPGARLIAAARL
                     GSVRLIDNIAA"
BASE COUNT          353 a          622 c          692 g          341 t
ORIGIN      
        1 tggcgagcat ctgccgaacc gcgagaagca tggcaagcag ctcgttgcgg tgtggcacag
       61 cgaggtgggc cagctgaacc agttcatcca cgtgtgggcg tatgacaatt tcgagcagcg
      121 cacgaccgtg cgcgccgccg cgatgaaagc gggcggttgg ccgccaccga tcggcgagtt
      181 cctggtcgag atgaagaacg agatcttcta cccggcaccg ttctcacccc cgctggaagc
      241 gcgcgagatc ggcccgctgt tcgaaatccg gcaatacacg ctggcgcccg ggtcaattcc
      301 gggcctgatc gagcgctggt cggagaagat cgaagggcgg cagaagttct cgccgctggt
      361 cgccggcatg tacaccgagt tcggtgcgct gaacaaatgg gtgcacatct gggcctacaa
      421 ggacgcgaac gagcgcaatt cggtgcgtgc cagcgcagcg gcgaccgggc agtggccggc
      481 acgtaacccg cctggcgtgg tgatcaagca ggagaacgcg ttcgtgatgc cggcggcata
      541 ttcgccgatc cgttagccgc gggcattcag gcggcgcgtc ttcgctttga tgcagcagga
      601 cggtagcgtg acatgcaaac gctaccgctc agcccccggt ccgcaattgc cgcgtgaggg
      661 aaaatccgct acccgctggg aatggatcag aacctcgccg agaagctgac cgcctggcgc
      721 cgtcacctgc acgcgcaccc tgaactcacg ttgcatgaaa aggaaaccgc ggctttcgtc
      781 tgcgggcggc tgaccgaact gggcgtgtcg ttcgttgccg gtgtgggtgg ccatggcgtc
      841 gtggcgacga tccagcgcgg ccagtcaaac cgcagcgtgg gcctgcgggc cgacatggat
      901 gcgctgccga tcaccgaaac cactggtctg gggcatgcat cgaccaatcc tggcgtgatg
      961 catgcctgcg gccacgacgg tcacaccacg tcactgctcg gtgcggctgc gttgttgcag
     1021 caggacacgt cctggagcgg tacggtgcat ctggttttcc agccggccga ggagggcggg
     1081 ggtggtgcca aaagcatgat cgccgacggg ctgttcgagc gtttcccgat ggagcgcatc
     1141 ttcggctacc ataactggcc tggcctcgat gccggcgcgg tcgcggtgca tgattcagcg
     1201 gtgatggcgt cgggcagccg catcgaattc cacgtgaagg gccattcggg ccacgctgcc
     1261 ctgccgcatc tgacgcgcga tccgatggtc gcttcggcac acctgctgct agcactgcaa
     1321 tcgatcgtgt cacgcaacgt cgatccgttg gattcggcgg tgatatcagt tgcgaccatg
     1381 caggccggca ccgcggccaa ccaggtcccg ggcgaggcga tcatgcgtgg cacgatgcgc
     1441 accatgcgca gcgaaacgcg tgatctggtg gagcaggcga tcgagcgcgt cgcggccggc
     1501 gtcgcgcagg catgcgatgt cgcgatcgac gtgaccatgc gacgcggcaa tccggtcacg
     1561 gtgaattcgg ttgctgaacg tgacctagct gctgacgccg tggtcgcagc gggactgccg
     1621 ctgcgccgcg acattccgcc ggctatgacc ggcgaggatt tcgcctggta tctgcaacag
     1681 cgccctggag ccttcgtctg gatcggcaat ggaccggcgg agaacgggcg ggagctgcac
     1741 aattcgggct acgactacaa cgacgcgatc ctgccggctg cgtccggctt cctcgccggc
     1801 gtggccaggc gcgcgctggg cagctgagcc gttacgcggc gatgttgtcg atcagcctta
     1861 ccgagccaag gcgggcggcg gcgatcagtc gtgcgcccgg ctgacggacg gagatggccc
     1921 gcagcgaggg gccgtcgacc agcgccagat agtcgacctc gaagccggca tcgttcaggc
     1981 gcgtttgcgc cgtcttcaat acaggcta
//
LOCUS       DATGEM010000470         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1607782, whole genome shotgun sequence.
ACCESSION   DATGEM010000470 DATGEM010000000
VERSION     DATGEM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607782"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VKQ27_14775"
     CDS             <1..503
                     /locus_tag="VKQ27_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90243.1"
                     /translation="FMEASLWGGKMNVRYGAPAMPFNAPFKLGPFTVDATGRLAPLES
                     GAPPVFLYRWHGRLMRARLAQRNAPAEGLALLTEVGLGRVPSTAGTLDDAARPRSFTL
                     VRWLSRAVPAGWHLRLAPDHRLWLEAETPIGLPITAAALLTQITCFALELGPYLDLLD
                     ETGVNA"
     gene            complement(493..1311)
                     /locus_tag="VKQ27_14780"
     CDS             complement(493..1311)
                     /locus_tag="VKQ27_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90244.1"
                     /translation="MPIPDLHSFHAPLLAFAVALALAGAARFLRVGILAAAAGGAGVL
                     AGWYAITGVLWVAAPRISIDDLTQIAAAALLIGLVVGRFAEGSAGTVGTLVAALFAAW
                     WLTGAPRHLAGVRAGWPVGLGTALATLLLVRLLMTGAQQLLSAALAGITLAASLYVAG
                     VPSVWLQLALVPALAALAMCLLPPMPGLAVLPVAADIAALSCLVVLAVGRLPRLGIGT
                     IDVAAASPFLALWLVPRVTQRFGFAGRAAPLAAGVLSGAVSVGLTWATRAVLRH"
     gene            complement(1348..2169)
                     /locus_tag="VKQ27_14785"
     CDS             complement(1348..2169)
                     /locus_tag="VKQ27_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLY90245.1"
                     /translation="MKHWRIEDVAWDRFDPTKVDPDLVPLVKAAAMVERNGTDYAVYL
                     NRVFGDDPDFRQAADYWADEEVQHGDALGKWAMLADPSWDYQSAFARYKAGYKLPLDA
                     EASVRGSRTGELIARCMVETGTSSYYTALGEAAGEPVLKQICRHIAADEFRHYKLFYD
                     HMRRYLARENISLLRRLRIAAGRIGESEDDELAYAWHCGNEPDDTTYEHERCSAAYMA
                     RAMAFYRFHHIERGMGMIFKAVGLKPRGRLSDLSAKAAWQLMQWRRQRIAAKLAA"
     gene            complement(2631..3050)
                     /gene="atpC"
                     /locus_tag="VKQ27_14790"
     CDS             complement(2631..3050)
                     /gene="atpC"
                     /locus_tag="VKQ27_14790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HLY90246.1"
                     /translation="MPVNLEIVSPERLLMSRPVDMVVIPASEGDMGVLEGHSPAIVML
                     RGGIIALYDGEQVTDQMYVAGGFAEVTPERCTVLANEVLAPADLSREEGERRLTAAQE
                     ALAQVDGNDVVAEEQALERVQSAQALIEAVDALLTGR"
     gene            complement(3067..>4265)
                     /gene="atpD"
                     /locus_tag="VKQ27_14795"
     CDS             complement(3067..>4265)
                     /gene="atpD"
                     /locus_tag="VKQ27_14795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLY90247.1"
                     /translation="TVPVGPETLGRILNVIGEPIDEQGPINTKMRYPIHRDAPSFEEQ
                     ATSAEVLVTGIKVVDLLAPYLKGGKVGLFGGAGVGKTVIIQELINNIAKAHGGVSVFA
                     GVGERTREGNDLYHEMIDAKVIMMDDHHKITEGSKVALMYGQMNEPPGARARVGLSGL
                     TMAEYFRDEEGQDVLFFVDNIFRFTQAGAEVSALLGRIPSAVGYQPTLATDMGALQER
                     ITSTKKGSITSVQAIYVPADDLTDPAPATSFAHLDATTVLSRQIAELGIYPAVDPLDS
                     TSRSLDPRIVGEEHYQVARETQRILQTYKSLQDIIAILGMDELSEEDKLVVARARKIQ
                     RFLSQPFHVAEVFTGFPGVFVPIADTIRSFKAVCSGEYDHLPEAAFYMVGTIEDAVKK
                     AEGMKA"
BASE COUNT          743 a         1393 c         1392 g          737 t
ORIGIN      
        1 ggtttatgga agcttcactt tggggcggca agatgaacgt caggtacgga gcgccagcca
       61 tgccgttcaa tgcacccttc aagctcggac cgttcacggt tgatgcgacc gggcgcctgg
      121 cgccattgga gtcaggagcc ccacccgtat tcctttaccg ctggcatggc cgactgatgc
      181 gggcgaggct cgcacagcgg aacgcgcccg ccgaagggct ggctctgctc accgaggtcg
      241 ggctcggccg cgtacccagc accgccggaa cgcttgacga tgcagcccgc ccccgcagct
      301 ttacgctggt gcgctggctg tcccgtgcag tgccagccgg ctggcacctg cgattggcgc
      361 ccgaccatcg gctctggctg gaggctgaaa cgcccattgg cctgccaatc accgctgcgg
      421 cgctgctgac ccagatcacc tgtttcgcac tcgaactcgg gccctatctc gatctgctgg
      481 atgagacggg ggtcaatgcc tgagcacggc tcgggtcgcc caggtcagtc caaccgacac
      541 tgcgccagac aggacaccgg cggccaatgg tgccgcgcgg ccggcgaaac cgaaccgttg
      601 ggtgacgcga ggtacaagcc acagcgccag gaatggtgag gcagcggcga catcgatcgt
      661 gccgatgccc agtcgcggta gccgtcccac cgcaaggacc acaaggcacg acagggccgc
      721 gatgtcggca gctaccggca ggactgccag acccggcatg ggtggcagca agcacatggc
      781 cagtgctgcc agggcgggaa ccagtgccaa ttgcaaccaa acggacggca cccctgcgac
      841 ataaagcgac gcggccaacg tgatgcctgc gagcgccgcc gacagcagct gctgtgcgcc
      901 tgtcatcaac aagcggacca gcagcaatgt cgccaacgcg gtacccaggc caactggcca
      961 accggcacgc accccagcga ggtgacgggg cgctcccgtc agccaccatg cggcaaacag
     1021 cgccgccacc agcgtaccca ctgtcccggc ggacccctcg gcaaaccgcc cgaccaccag
     1081 cccgatcagc agcgcagctg cggcgatctg cgtcaggtca tcgatcgata tccgcggcgc
     1141 tgcgacccag agcacgcccg tgatcgcata ccatccggcc agcacaccgg cgccacccgc
     1201 agccgctgcc agaattccga cgcgcaggaa ccgggccgct ccggcaagcg ccaacgcgac
     1261 ggcgaaagcc agcagcggcg cgtggaatga gtgcaggtcg ggtatcggca caggtgacgt
     1321 ggcggtcgct gcgttcccgg atgcgttcta ggcagccagc tttgcggcaa tacgctgccg
     1381 tcgccactgc atgagctgcc aggctgcctt tgccgacagg tcggacaggc ggccacgtgg
     1441 cttcaggccg acggctttga agatcatgcc catgccgcgc tcgatgtggt ggaagcgata
     1501 gaaggccatc gcacgtgcca tgtaggctgc cgagcagcgc tcgtgctcat aggtggtatc
     1561 gtccggttca ttgccgcagt gccaggcata ggcgagctca tcatcctcac tttcgccgat
     1621 acggccggcg gcgatccgga ggcgccgcaa gaggctgatg ttctcgcgcg ccagatagcg
     1681 gcgcatgtgg tcatagaaca gtttgtagtg gcggaattcg tcagccgcga tgtggcggca
     1741 gatctgcttc agcaccggct cgccggccgc ctcgccgagg gcggtgtagt agctggacgt
     1801 gcccgtttcc accatgcagc gggcaatcaa ctcaccggtg cgtgagccac ggaccgaagc
     1861 ctccgcatcg agcggcagct tgtagccggc cttgtagcgg gcgaaggcgg attggtagtc
     1921 ccacgacggg tcggcgagca tcgcccattt gccgagcgcg tcgccgtgct ggacttcctc
     1981 atcagcccag tagtcggcgg cctggcggaa gtccgggtca tcgccgaaca cgcggttgag
     2041 gtagacggcg tagtcggtgc cgttgcgctc caccatggcg gcggctttga ccaaggggac
     2101 cagatcggga tcgaccttcg tgggatcgaa gcggtcccag gccacatcct cgatgcgcca
     2161 atgcttcatg gtgtgttcca cacggttgga cgcagcggcg gaaggttgcc acgggttggc
     2221 gggattcgaa agggtgggtg cttatggtat gctgcaccgt gatgagcacc ggaacgcgcc
     2281 acatcacaac atgcgggtcc cggatctggc ggtgacatgt gctgatgtgg atgctcgcca
     2341 gatagccttg cccgaggccc tgctcattgt ggagatcctg tcgccgacgg acgcatcaga
     2401 cacgtgggag aacgtcttga cctacgtatc tatccccagt ggttcaggaa ctgctggcgc
     2461 tccggtctgc ctcaatcgcc gcagacctgc tacggcggcg tgcggatggt atgtggccgg
     2521 gggagccgac acaggttggg cgtgatgata tgctgcacct ggcgagcgtg gacctgagct
     2581 gtctgctcac cgagttcgac gtcggaactt acctcgcgcg acagacctga tcagcggccg
     2641 gtcagcagag catccaccgc ttcgatcagc gcttgtgccg attgcacacg ctccagagcc
     2701 tgttcctcgg cgacaacatc gttgccatct acctgggcga gcgcttcctg agcggcggtg
     2761 aggcggcgtt cgccttcctc ccgtgaaagg tcggccggcg ccagcacttc gttggccagc
     2821 accgtgcagc gctccggggt gacttcggcg aaccccccgg cgacgtacat ctggtcggtg
     2881 acctgctcgc cgtcatagag ggcgatgatc ccgccacgca gcatgacgat ggcgggagag
     2941 tggccttcga gcacgcccat gtcgccctcg gaggcaggga tcaccaccat gtccaccggc
     3001 cgcgacatca ggaggcgttc ggggctaacg atttccaggt tcactggcat gggttcaccg
     3061 gtcgccttat gccttcatgc cttcagcctt tttcaccgcg tcctcgatcg tgccgaccat
     3121 gtagaacgcc gcttccggca ggtggtcgta ctcgccggag cacacggcct tgaagctgcg
     3181 gatcgtgtcg gcgatcggca cgaacacgcc ggggaacccg gtgaatacct cggccacgtg
     3241 gaagggctgc gacaggaagc gctgaatctt gcgcgcgcgg gcaaccacaa gcttgtcctc
     3301 ttccgacagc tcgtccatgc ccaggatcgc gatgatgtcc tgcagcgact tgtaggtctg
     3361 caggatgcgc tgcgtctcgc gggccacctg gtagtgctcc tcgccgacga tgcgcgggtc
     3421 cagtgagcga gacgttgagt ccagcgggtc caccgccgga tagatgccca attcggcgat
     3481 ctggcggctc agcaccgtcg tcgcgtccaa gtgggcgaac gaggtggcag gcgccggatc
     3541 ggtcagatcg tcggcgggca cgtagatcgc ctgcaccgac gtgatcgagc ccttcttcgt
     3601 tgacgtgatc cgctcctgca gcgcgcccat gtcggtggcc agcgtcggct gataacccac
     3661 cgcggagggg atgcggccca gcagcgccga cacctcggcg cctgcctggg tgaagcggaa
     3721 gatgttgtcc acgaagaaca gcacgtcctg gccttcctcg tcgcggaagt attcggccat
     3781 cgtcaggccg gacaggccga cgcgggcacg ggcgcccggc ggctcgttca tctggccgta
     3841 catcagcgcc accttggagc cttcggtgat cttgtggtgg tcatccatca tgatcacttt
     3901 ggcatcgatc atctcgtggt agaggtcgtt gccttcgcgc gtgcgctcgc ccacgcccgc
     3961 gaacaccgac acgccaccat gcgccttggc gatgttgttg atcagctcct ggatgatcac
     4021 ggtcttgccc acgccggcgc cgccgaacag gccgaccttg ccgcccttca ggtagggggc
     4081 caggaggtcc accactttga tgccggtgac caggacttct gccgacgtcg cctgctcttc
     4141 gaaggatggc gcatcgcggt gaatcggata gcgcatcttc gtattgatcg ggccctgctc
     4201 gtcgatcggc tcgccgatca cgttcaggat gcggccaagc gtttcggggc cgacgggcac
     4261 ggtga
//
LOCUS       DATGEM010000471         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_580177, whole genome shotgun sequence.
ACCESSION   DATGEM010000471 DATGEM010000000
VERSION     DATGEM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580177"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VKQ27_14800"
     CDS             <1..386
                     /locus_tag="VKQ27_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLY90248.1"
                     /translation="TEWARGNEIERRMEGSDLGGTMRLGAYPAVLTPGSLVSELYGDA
                     TRIEERHRHRYEVNVHYKEALELAGLRFCGMSPDGVLPEIIELPDHPWFVGVQYHPEL
                     KSKPFEPHPLFAGFVAAAVRQARLV"
     gene            complement(390..827)
                     /locus_tag="VKQ27_14805"
     CDS             complement(390..827)
                     /locus_tag="VKQ27_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY90249.1"
                     /translation="MILLDTNVISELVRRQPEPAVETFLQSQPPLSIYTASVCEAEIR
                     YGLARMPAGRRRNELTRRTIELFATAFPDRILTFDSSAADLYGVIRASRGRAGRPISV
                     EDAMIAAIARAYGVTIATRNEHDFVGCGVHVINPWSPGALQQQ"
     gene            complement(824..1090)
                     /locus_tag="VKQ27_14810"
     CDS             complement(824..1090)
                     /locus_tag="VKQ27_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HLY90250.1"
                     /translation="MPSLLIRNVDSALHSQLKRRAAAHHHSLEEEVRHLLRMAVASQP
                     REEHVVDAARSLFGPKHGVDLDIPPRGSAPERPPPDFSDFDPDR"
     gene            1156..1983
                     /gene="kdsA"
                     /locus_tag="VKQ27_14815"
     CDS             1156..1983
                     /gene="kdsA"
                     /locus_tag="VKQ27_14815"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HLY90251.1"
                     /translation="MNQVVHVGDTEIANHLPFVLIAGPCQIESRGHALEVSAALREMS
                     AETGVPVIYKSSFDKANRTSVSAARGLGIGDGLAVLAEVREATGLPTLTDVHTAEQCA
                     IAAQAVDVLQIPAFLCRQTDLLLAAGETGKPINVKKGQFLAPWDMANVAAKIASTGNQ
                     NVMLCERGASFGYNTLVNDFRALPIMAQTGYPVVFDATHSVQQPGGQGTSSGGQREFA
                     PVLARAALAVGVAAVFIETHPDPDRAPSDGPNMIALRDMPALIARLKAFDELAKRAA"
     gene            complement(2004..>2981)
                     /locus_tag="VKQ27_14820"
     CDS             complement(2004..>2981)
                     /locus_tag="VKQ27_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLY90252.1"
                     /translation="QTVIEISKPGGPEVLVPATRPVPEPNAGEVLIRIAAAGVNRADC
                     LQRMGRYPMPPGAPDIPGLECSGTIVKRGDGVTQWQDGDQICALMVGDGYGEYAAIPA
                     VQCLPVPQGVSLADAGGLPETFCTVWTNVFERMHLQPGETFMIQGGTSGIGVTSIQLA
                     KAWGCKVLATAGSDEKCTACRDFGADVAINYRTQDFVAEGKAATNGRGVDAILDMVGG
                     DYIPREINLLAHEGRLCFVALMGGTKVEADFGAIQRKHLTVTGSTLRSRSIEQKASII
                     AALREKVWPMWSQGKLRPFTYRQFPLREAAKAHALMESSEHIGKILLVP"
BASE COUNT          493 a          874 c         1059 g          555 t
ORIGIN      
        1 tgaccgagtg ggcgcgcggc aacgagatcg agcggcgcat ggaaggctcc gaccttggtg
       61 gcacgatgcg gcttggtgcg tatccggcgg tgctgacgcc tggttcgctg gtttccgagc
      121 tgtacggcga tgcgacgcgg atcgaggagc gccatcggca tcgctacgaa gtcaacgtgc
      181 actacaagga agcgctggag ctcgccggct tgcggttctg cggaatgtcg ccggatggtg
      241 tgctgccgga gatcatcgag ctgccggacc atccgtggtt tgtgggcgtg cagtatcatc
      301 cggagctgaa gtcgaagcca ttcgagccgc atccgttgtt cgccgggttc gtggcggcgg
      361 ccgtgcggca ggcgcggctg gtttagcggc tattgctgct gcagtgcacc cggagaccat
      421 ggattgatga catgcacacc gcatccgacg aagtcgtgct cattgcgtgt cgctatcgtg
      481 acgccgtagg cacgtgcgat ggcagcaatc atggcgtcct ccaccgagat tgggcggcct
      541 gcgcgcccgc gcgacgctcg gatcactccg tagagatcgg cagcggagga gtcgaaggtg
      601 aggatccggt cgggaaaggc cgtggcgaat agttcgatgg tccgacgcgt cagctcattg
      661 cgcctccgtc cggcaggcat gcgggcgagg ccatatctga tctcggcttc gcagacggag
      721 gcagtgtaga tggacagcgg cggctggctt tgcaggaacg tttcaacggc tggttccggt
      781 tgacggcgga ccagctcgga gatgacgttt gtgtccagca gtatcaccgg tcgggatcga
      841 agtctgagaa atccggtggt ggacgctcgg gcgcgctgcc acgaggcggg atgtccagat
      901 ctacgccgtg cttcgggccg aacaggctgc gcgcggcgtc gacgacatgt tcctctcgtg
      961 gttggctggc gacggccatg cggagaaggt ggcgcacctc ctcttccagg gaatggtggt
     1021 gtgccgcggc acgccgcttc aactggctat gcaacgcaga gtcgacattg cggatcaaca
     1081 ggctgggcat gcgcactttc ttcggctgct agcatatgct agcaatcgct tgtccaacga
     1141 tgcaacggtg atcccgtgaa tcaggtcgta cacgtaggcg atacggagat cgccaaccac
     1201 ctgccgttcg tgttgatcgc tggcccgtgc cagatcgaat ctcgcggcca tgcgctggaa
     1261 gtgtctgctg cgctgcgcga gatgtcggcg gagaccggcg tgccggtgat ctacaagtcc
     1321 agcttcgaca aggccaatcg aacgtcggtt tcagcggcgc gcggtcttgg gatcggagac
     1381 ggattggccg tgcttgccga ggtgcgcgag gctaccggat tgccgacgct gacagatgtg
     1441 catacggcgg agcaatgcgc gatcgcggcg caggcggtgg atgtgctgca aattccggcg
     1501 ttcctctgcc ggcagactga tctgctgttg gcggctggcg aaaccggcaa gccgatcaac
     1561 gtgaagaagg ggcagttcct ggcgccatgg gacatggcga atgtcgccgc aaagatagcc
     1621 agcacgggca accagaacgt catgctgtgc gagcgtgggg cgagcttcgg ctacaacacg
     1681 ctggtcaacg attttcgcgc gctgccgatc atggcgcaga ccggttatcc ggtggtgttc
     1741 gatgcgacgc actcggtgca gcagccgggc gggcagggga cctcatcggg ggggcagcgc
     1801 gagtttgcgc cggtgctggc acgggcggct ttggcggttg gtgtggcggc ggtgttcatc
     1861 gaaactcatc cggatccgga tcgcgcgcca agcgatggcc cgaacatgat tgcgctgcgc
     1921 gacatgccgg cgctgattgc gcggctgaag gcgttcgacg agctggcgaa gcgtgcggcc
     1981 tgatattcgc gtccagatga acgtcagggc accagcagga tcttcccgat gtgctcgctc
     2041 gactccatca gcgcatgcgc cttcgcggcc tcgcgcaggg ggaactggcg ataggtgaac
     2101 ggccgcagtt tgccctgcga ccacatcggc cacaccttct cgcgcagcgc ggcaatgatc
     2161 gaggccttct gctcgatgct gcgtgaacgc agtgtggagc cggtcaccgt caggtgcttg
     2221 cgctggatcg cgccgaaatc cgcctcgacc ttggtgccac ccatcagggc gacgaagcag
     2281 agacggcctt cgtgggcgag caggttgatc tcgcgcggga tgtagtcgcc gcccaccatg
     2341 tcgaggatcg catcgacgcc gcggccgttg gttgctgcct tgccctcggc gacgaaatcc
     2401 tgggtccggt agttgatggc gacatcggcg ccgaagtcgc ggcaggcggt gcatttctca
     2461 tcggagccgg cggtggcgag caccttgcag ccccatgcct tggcgagctg gatcgaggtc
     2521 acgccgatac cgctggtgcc gccctggatc atgaacgttt caccgggttg caggtgcatc
     2581 cgttcgaaca cgttggtcca gacggtgcaa aaggtttcgg gcaggccgcc ggcatcggcc
     2641 aggctgacgc cttgtggaac gggcaggcac tgcacagcgg gaatcgcggc gtattcgccg
     2701 tagccgtcgc ccaccatcag ggcgcagatc tggtcgccgt cctgccactg ggtgacgccg
     2761 tcgccgcgct tgacgatggt gcctgagcat tccaggccgg gaatgtccgg cgcgccgggc
     2821 ggcatcgggt agcggcccat gcgctgcagg caatccgcgc ggttcacgcc tgccgcggcg
     2881 atgcggatca ggacctcgcc cgcgttcggc tcggggacgg ggcgggttgc tgggacaagg
     2941 acctcagggc cgcctggctt gctgatctcg atgaccgtct g
//
LOCUS       DATGEM010000472         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_327238, whole genome shotgun sequence.
ACCESSION   DATGEM010000472 DATGEM010000000
VERSION     DATGEM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327238"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2056
                     /locus_tag="VKQ27_14825"
     CDS             <1..2056
                     /locus_tag="VKQ27_14825"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY90253.1"
                     /translation="AQDPHSCGMYRVAFDVGGTFTDFTVHETATGALRHFKVPSTPAD
                     PSEAIEAGLRALIDAQQIAPAEISFVGHGTTIATNMVIERRGVTTGLITTRGFRDVLE
                     IGRQVRPHLYDYTVRIPLPIVPRARRAEVEERIDAGGAVLRALDEAQVAAAADALAAQ
                     DVQAIAICFLHSYLNDAHERRAAEIVRCRLPDVYVCTSSEVLPEFREYERFSTAAINA
                     YIGPRMARYLDRFLGRLAGLGIGVQPYTVHSNGGLMSVESVRRFPVRCCLSGPAAGVV
                     GAAEVARDAGLPNVITFDAGGTSTDVSLIERGRPAFTSGRSIADYPVRAPMVDVHVIG
                     AGGGSIAWLDDAGALKVGPHSAGAAPGPVAYGKGGTEPTLTDANAVLRRLNPVALLDG
                     RMQIDERAAHQAIAEKIAAPMGLSVEQAALGIISIAVANMSRAIRSVSTEKGHDTREF
                     VLFPFGGAGPLHATAVAVECGIQRVLVPVQPGTMCARGILLSDVSIDLVRSELLEATE
                     DNWPRILQRFVTMRSDGAAWLESEQIALERRRFDCVIDARYVGQNHEVPVRLSSDAVP
                     RDDFIRAFEDAHRREYGYDIAGRAIEVVNCRLKAVGLIDRPSPRFAGSSAAPLPKAVR
                     RVHFDEGWLETPVFDREALPIGANIRGPAVIEEMSATTLLPPGCEMTVTRSGNLLLEI
                     GA"
     gene            2053..>2400
                     /locus_tag="VKQ27_14830"
     CDS             2053..>2400
                     /locus_tag="VKQ27_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY90254.1"
                     /translation="MSELGDPIAMEVFSNRLLSITEDMGNTLIRSSFSTNIKERKDCS
                     VGLFDARGRLIAQASHIPLHLGSLLGSVQAVLARHVVEAMRDGDAFVLNDPYLAGGTH
                     MPDISIVTPIVIDG"
BASE COUNT          368 a          719 c          871 g          442 t
ORIGIN      
        1 cgcccaggac cctcacagtt gcggcatgta tcgcgttgca ttcgacgtcg gtggcacgtt
       61 caccgacttc accgtccatg aaacggccac tggggcgttg cggcacttca aggtgccgtc
      121 cacgccggcc gacccgtcgg aggccatcga ggcgggcctg cgtgcgctga tcgacgcgca
      181 gcagatcgcg ccggcagaga tcagcttcgt cgggcacggc acgacgatcg ccacgaacat
      241 ggtgatcgag cggcggggcg tgaccacggg tctgatcacc acgcggggat ttcgcgatgt
      301 gcttgagatc gggcggcagg tgcgtccgca tctctatgac tacacggtgc gaataccgct
      361 gccgatcgtg cctcgggcgc gccgggcgga ggtggaggag cggatcgatg ccgggggcgc
      421 tgtgctgcgt gcgctcgatg aggcgcaggt ggctgcggcc gcggatgcgc ttgcggcgca
      481 agacgtgcag gcgatcgcga tctgcttcct gcacagctat ctgaacgacg cgcatgaacg
      541 gcgcgctgcg gagatcgtgc gctgcaggct gcctgacgtc tatgtctgca cgtcgtccga
      601 ggtgctgcct gagttccgcg agtacgagcg gttctccacc gccgcgatca atgcgtatat
      661 cggtccgcgc atggcgcgat acctggaccg gttccttggc cggctcgccg ggctgggcat
      721 cggggtgcag ccctacaccg tgcattccaa tggcgggctg atgtcggtgg agtcggtgcg
      781 acggtttccg gtccggtgct gcctgtccgg tcctgctgct ggcgtggtcg gtgcggcaga
      841 ggttgcgcgc gacgcgggtt tgcccaacgt gatcaccttc gatgcgggtg gcacatcgac
      901 cgatgtgtcg ctgatcgaac gcgggcggcc ggcgttcacg tcgggccgct cgatcgccga
      961 ttatcccgtg cgggcgccga tggtggacgt gcatgtgatc ggcgcgggcg ggggctcgat
     1021 cgcatggctc gatgatgccg gcgcgctgaa ggttgggccg cacagcgcgg gagcggcgcc
     1081 cggcccggtc gcgtatggca agggcggcac cgaacccacg ctgaccgacg ccaatgcggt
     1141 actgcgacgg ctcaatccgg tggctctgct ggatggccgc atgcagatcg atgaacgcgc
     1201 tgcgcatcag gcgatcgcgg agaagatcgc agcaccgatg gggctgtcgg tggagcaggc
     1261 ggcgctcggc atcatcagta ttgccgtggc gaacatgagc cgtgcgatcc ggtccgtgtc
     1321 gactgaaaag ggacacgata cgcgcgagtt cgtgctgttt ccctttggtg gtgccggtcc
     1381 gttgcatgcg accgcggtgg ccgttgaatg cggtatccag cgtgtgctgg ttccggtgca
     1441 gcctggcacg atgtgcgcgc ggggcattct gctctcggat gtcagcatcg acctggtgcg
     1501 gagcgagttg ctggaagcga ccgaggacaa ctggccgcgc atcctgcagc gctttgtcac
     1561 gatgcggtcg gacggtgcgg cgtggctgga gagcgagcag attgcgctgg agcggcggcg
     1621 cttcgattgc gtcatcgacg cacgttacgt ggggcagaac catgaggtgc cggtccggct
     1681 ttccagcgac gcggtcccac gcgacgactt tatccgtgca ttcgaggacg ctcatcgccg
     1741 cgaatacggt tacgacatcg ccggccgcgc gatcgaagtg gtgaattgcc ggctcaaggc
     1801 ggtgggcctg atcgaccggc catcgccgcg ctttgccggc agcagtgcgg cgccgctgcc
     1861 gaaagccgtg cgccgcgtgc atttcgatga ggggtggctg gaaacgccgg tgttcgatcg
     1921 cgaggctctc ccgattgggg cgaacatccg ggggcctgcg gtcatcgagg aaatgagcgc
     1981 gacgacactg ctgccgccgg gctgtgaaat gacggtcacc cgctccggca acctgctgct
     2041 ggagattggc gcatgagcga actgggcgat cccatcgcga tggaggtgtt cagcaatcgc
     2101 ctgctgtcga tcaccgagga catgggcaac acgctgatcc gctcgtcgtt ctccaccaac
     2161 atcaaggagc gcaaggactg ctcggtgggt ctgttcgatg cgcggggacg gttgatcgcg
     2221 caggcgtcgc atataccgct gcatctcggc tcgctgctgg gcagcgtgca ggcggtgctg
     2281 gctcggcacg tcgtagaggc gatgcgcgac ggggatgcgt tcgtgctgaa cgatccgtac
     2341 ctcgcgggcg gcacgcatat gccggacatt tccattgtca cgcccattgt catcgatggc
//
LOCUS       DATGEM010000473         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_428976, whole genome shotgun sequence.
ACCESSION   DATGEM010000473 DATGEM010000000
VERSION     DATGEM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428976"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..460
                     /locus_tag="VKQ27_14835"
     CDS             140..460
                     /locus_tag="VKQ27_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90255.1"
                     /translation="MTLSFNLKAGMSIGEAANAIRVLERQLGKPASLATSFQGNAQAF
                     ESSLSKEPVLIAAAIVVIYIILGTGTGSEIRQSLGYTIVGGLLLSQLLSCTRRRWRIC
                     IWIG"
     gene            568..1425
                     /locus_tag="VKQ27_14840"
     CDS             568..1425
                     /locus_tag="VKQ27_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HLY90256.1"
                     /translation="MLSRRLLLTAAVLSTTASASGSFQSFLADMRSQARRAGIAPATL
                     DRAFAGLQPNQKVLDRDNHQPEFTLTWAKYRAMLITDQRIASGRAAYQQNRALFARVR
                     DVYGVDPGVILGIWGLESSFGTGMGDFHVIEALATLAWEGRRASFFRGELMAALHILD
                     HGDVTPAHMMGSYAGAMGQPQFMPSSYLRYAVDFEGSGKRDIWTSKPDVLASIANYLK
                     QSGWHGGEPWGEQVSGQQTGTAGKLVQPDGPGGESFVVFGNFNAIRRYNPSDYYALAV
                     GLLGDAAVG"
     gene            1422..2288
                     /locus_tag="VKQ27_14845"
     CDS             1422..2288
                     /locus_tag="VKQ27_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLY90257.1"
                     /translation="MRRAFLLLLIGPLACCHQVTPSPHYVLGAPYQAGGGWYYPRENF
                     ALQETGLATVYGPNHPDLTADGEVFDQSALAAAHQTVQLPAIARVTNLENGRQVVVRI
                     NDRGPATPHRVLEVTRRTASLLGFPPDNVARVRIDLLADQSQAAVDAVPGAPSLQIAA
                     APRGEVTQTDLPAPGSAAAAPAVVAPAATAHSVPASNAPPLRLPETVTQGQPNRGTLY
                     VELSNFQNYRYAIMQRARVAALGANVVSQRENGTEVYRVLIGPLNGVQQADSVLDQVI
                     ASGVTDARIVVE"
     gene            2304..>2990
                     /locus_tag="VKQ27_14850"
     CDS             2304..>2990
                     /locus_tag="VKQ27_14850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLY90258.1"
                     /translation="MLTRRSALLASVCFIAAAAPAASQPRRPAPTPRPGGARGKKEAA
                     VPTDPAATPIGPVDTTARWAYGQDFNTGATLLEKAADDHMPPSSMTKLMTIYLVYERL
                     KQGKMKLDDQLLVSEKAWRMGGSKMFVQVGTQVAVEDLIRGLIVDSGNDACIVLAEGI
                     AGSEEQFVEMMNAKAKLLGMTNTSYRNCTGWPDPDQYMSCRDIATLARHIIMDFPQYY
                     HYDSEKTFKYN"
BASE COUNT          523 a          935 c          979 g          553 t
ORIGIN      
        1 caggacacca gcatgatcat cgcgatcgcc gaagccgcgc aggacatctc gttcaccggc
       61 atgagcgacg catggaggcg gtaatccgcg tcgcactgaa ggatccggcg gtcgcctcgg
      121 ttgggtcgca gatcggtcgg tgacgctgtc gtttaacctg aaagctggca tgtcgatcgg
      181 tgaggcggct aatgccatcc gggtgctgga acggcagctt ggcaagcccg catcgctggc
      241 cacgagtttc caaggcaatg cgcaggcgtt cgagtcgtct ctgtcaaagg agccggtcct
      301 gatcgcggcg gcaattgtgg tgatctatat catcctgggc acgggcaccg gctcggagat
      361 ccgccagtcc ctgggctaca cgatcgtcgg cggactgcta ctgtcgcagt tgctgagctg
      421 tacacgacgc cggtggcgta tctgtatctg gatcggctga gcgcgcggcg aagacgcgcc
      481 cgagtcgtgt catgacgaaa gcggaaggtg ctcgcgctta ggccaatctg attgcctctc
      541 gcttgtcgcc tatggcaaac agcggacatg ctgagtcgtc gcctgttgct cactgccgcc
      601 gtcctgtcca ccactgccag cgcgtcaggc agctttcaga gcttcctcgc ggacatgcgc
      661 agccaggcgc gccgggccgg catcgcccct gccacgctgg accgcgcttt tgctggcttg
      721 caacccaacc agaaggtgct ggatcgcgac aaccatcagc cggaattcac tctgacctgg
      781 gcgaaatacc gcgcgatgct gatcaccgac cagcgcattg cgagcggccg tgcggcctat
      841 cagcagaatc gggcgctgtt tgctcgcgtg cgggacgttt atggagtcga tccgggcgtg
      901 atcctcggca tctggggcct cgagtcgagc ttcggtaccg gcatgggtga cttccatgtc
      961 attgaggcgc tggcgacgct ggcgtgggaa ggccgccgcg ccagcttttt ccgcggcgag
     1021 ctgatggccg cactgcacat cctggaccat ggcgacgtga cacccgcgca tatgatgggc
     1081 tcgtatgccg gggcgatggg gcagccgcag ttcatgccca gctcctactt gcgatatgcc
     1141 gtggacttcg agggctccgg caagcgcgac atctggacca gcaagccgga cgttctggct
     1201 tcgatcgcca actatctgaa gcagtccggc tggcacggtg gcgagccgtg gggcgagcag
     1261 gtttcagggc agcagacggg gacggcaggc aagctcgtgc agcctgatgg cccgggtggt
     1321 gaatcatttg tcgtgttcgg caatttcaat gcgattcgtc gctacaaccc gtctgactac
     1381 tacgcgctcg cagtcggcct gctcggcgac gcggccgtcg ggtgagacgc gcgtttctcc
     1441 tgctactgat cggcccattg gcctgctgcc atcaggtaac tccgagcccg cattatgtgc
     1501 ttggggcgcc gtatcaggca ggcggcggct ggtactatcc gcgcgagaat tttgccctcc
     1561 aggagacggg gctggcgacc gtctatggtc ccaaccatcc tgatctgaca gccgacggcg
     1621 aggtcttcga tcagtccgcg ctggcagcgg cgcatcagac cgtgcagctt cccgcgatcg
     1681 cccgcgtcac caatctcgag aacggccgcc aggtcgtggt gcgcatcaac gatcgagggc
     1741 ccgccacacc gcaccgggtg ctggaagtga cgcggcgtac cgccagcttg ctcggttttc
     1801 cgcccgacaa tgttgcgcgg gtgcgaatcg atctgctggc ggatcagagc caggccgcgg
     1861 tcgatgccgt acctggcgcg ccgagcctgc agattgccgc tgcgccgcgc ggcgaggtga
     1921 cgcagaccga tctgcctgcc ccaggcagtg cagctgccgc gccggcggtc gtagccccgg
     1981 ccgccaccgc gcatagtgtc cctgcgtcca atgcgccgcc tctgcgcctg ccggagacgg
     2041 tcacgcaggg ccagccgaat cgtgggaccc tctacgtcga gctcagcaat ttccagaact
     2101 accggtacgc catcatgcag cgggcgcgag tcgcggcgct gggtgccaat gtggtgagcc
     2161 agcgcgagaa tggcacggaa gtctatcgcg tgctgatcgg cccgctgaat ggcgtgcaac
     2221 aggcagactc tgtgctggac caggtgatcg cctcgggagt aaccgatgcg cggatcgtgg
     2281 tggaatagga gtcgagtctg accatgctca cccgtcgttc tgccttgttg gcgtcggtgt
     2341 gctttatcgc tgctgcggcg ccggccgcat cccagccacg tcggccggcg cccacgccgc
     2401 gtcccggtgg tgctcgcggc aagaaggagg cggcagttcc cactgatcct gccgccacgc
     2461 cgatcgggcc ggtggatacc accgcgcgct gggcctatgg ccaggacttc aacacgggcg
     2521 ccacgctgct ggaaaaggct gcggatgacc acatgccgcc gtcgtcgatg accaagctga
     2581 tgacgatcta cctggtctat gagcggctga aacaaggcaa gatgaagctc gatgaccagt
     2641 tgctggtcag cgagaaggcg tggcgcatgg gcggctcgaa gatgttcgtc caggtcggga
     2701 cgcaggttgc ggtggaggac ctgatccgcg gtcttatcgt cgactcgggc aacgatgcct
     2761 gcattgtgct ggccgagggc attgccgggt ctgaagagca gttcgttgag atgatgaacg
     2821 ccaaggccaa gctgctcggc atgaccaaca ccagctatcg caactgcacc ggttggcccg
     2881 accccgacca atacatgagc tgccgcgaca tcgccacgct ggcgcggcat atcatcatgg
     2941 acttcccgca atattatcat tacgactcgg aaaagacatt caagtacaac
//
LOCUS       DATGEM010000474         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_686043, whole genome shotgun sequence.
ACCESSION   DATGEM010000474 DATGEM010000000
VERSION     DATGEM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686043"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VKQ27_14855"
     CDS             <1..570
                     /locus_tag="VKQ27_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HLY90259.1"
                     /translation="MPWGVKVSRVEIRKIEPPENLIRAMNLQMTAERERRAVVARADG
                     EREAAIKRAEGEKQALILQAEGRQQAAIRDAAARVQLATAEGEATRLVSEASTRYGPA
                     GLRYFIADRYVRAFQSLASAPNTRLVVVPMESSALAGGIVQAMQFLRGDEGGPPGATP
                     TTMPPPSEPPPPPAPHIPPGSPWGAPAPS"
     gene            567..983
                     /locus_tag="VKQ27_14860"
     CDS             567..983
                     /locus_tag="VKQ27_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HLY90260.1"
                     /translation="MNDWIIWLAAGLALLLLEALAPGAFMMWLGLAACGTGLVALASG
                     IGFELQVITCAVLAAISLSIGLRLRHRPARLNTQQAGLAGRSATALSFRGREGRVRLG
                     DSDWAARVPPDVPEPHEGTRLRVEGVDGTVLIVRPE"
     gene            991..1383
                     /locus_tag="VKQ27_14865"
     CDS             991..1383
                     /locus_tag="VKQ27_14865"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY90261.1"
                     /translation="MDSDTAKRLVQDVEDAFGAADLARIEQGFTEDAVARFADFPEMR
                     GRDAVMTFLRARFARTKGYRLRKTLHCVMGDTLANTWDASWEDAQTGKQMLGRGTEIW
                     IVRDGRIALWDATFNAWEKGGKPTTPVV"
     gene            complement(1387..1803)
                     /locus_tag="VKQ27_14870"
     CDS             complement(1387..1803)
                     /locus_tag="VKQ27_14870"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY90262.1"
                     /translation="MADISAISQRFFEACETGRGWEGCQAYCTPDASFAAQAEPLANV
                     KTLREYCDWMKGLLTFMPDGRYTVKSFAVDEARSNVSAYGVFQATHTGEGGPCPPTGK
                     STTTDYVYVMQFTGEKISHMTKIWNAGWAMRELGWG"
     gene            complement(1890..2144)
                     /locus_tag="VKQ27_14875"
     CDS             complement(1890..2144)
                     /locus_tag="VKQ27_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLY90263.1"
                     /translation="MARAAKKKQPAKAKAKAKAKAGTTARQKQPDWNSLKVDWPQAKQ
                     AISLRIDQDVLDWFRGGGAGYQTRMNAVLRAFVESQRGKR"
     gene            complement(2821..>3432)
                     /gene="fusA"
                     /locus_tag="VKQ27_14880"
     CDS             complement(2821..>3432)
                     /gene="fusA"
                     /locus_tag="VKQ27_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554874.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLY90264.1"
                     /translation="PHTETYTHKKQTGGSGQYAEVKIVFEPLERNAGVVFENKVVGGS
                     VPKEYIPAVEKGIAVQAETGVLAGFPTVDFKYTLVDGKYHDVDSSALAFEIAAKSCFR
                     DGMKKATPIILEPVMDVEVTTPSDHVGDVVGDLNRRRGMIQNQESSGSTVIVRAHVPL
                     KEMFGYISDLRSMTKGRASFTMQFHHYDPVPRNIADEIMAKTA"
BASE COUNT          584 a         1172 c         1079 g          597 t
ORIGIN      
        1 atgccgtggg gcgtgaaggt cagccgggtg gaaatccgca agatcgagcc accggaaaat
       61 ctgatccgcg cgatgaacct gcagatgacc gccgaacgcg aacgccgcgc cgtggtggcc
      121 cgcgccgacg gcgagcggga agccgcgatc aagcgcgccg agggtgagaa gcaggcgctg
      181 atcctgcaag cggaagggcg gcagcaagcc gccatccgcg acgccgcggc ccgcgtgcag
      241 ctcgccacgg ccgagggcga agcaacacgg ctggtctctg aagcatccac ccgctacggc
      301 cccgcgggcc tgcgctactt catcgctgat cgttatgtgc gggcgttcca gtcgcttgcg
      361 tcagcgccga acacgcgcct ggtcgtggtg ccgatggaaa gcagcgcgct cgccggcggg
      421 atcgtacagg cgatgcagtt tctgcgaggc gatgaaggcg gcccaccggg agcgacgcca
      481 acgaccatgc cgccgccgtc ggagccgccg ccccctccgg cgccacacat cccaccaggc
      541 tcgccgtggg gagcgcccgc gccctcatga acgactggat catctggctg gcggccgggc
      601 tcgccctgct gttgctggag gcattggcgc ctggcgcgtt catgatgtgg ctcgggctgg
      661 ccgcctgcgg caccgggctt gtcgcactgg ccagcggcat tggcttcgaa cttcaggtga
      721 tcacgtgcgc ggtgctggcg gcaatctcgc tcagcatagg cctgcgcctc cgtcaccgcc
      781 ctgcccggct gaacacccag caggccggtc tcgcaggccg ttccgccacc gcgctgtcct
      841 tccgggggcg cgaaggccgc gtgcgcctcg gcgacagcga ttgggccgcg cgcgtcccac
      901 cggacgtgcc agagccccac gaaggcaccc ggttgcgcgt ggaaggcgtc gatgggacgg
      961 tgctgatcgt acggccggag tgatgatcca atggacagcg acaccgcgaa acgcctggtg
     1021 caggatgtcg aggacgcatt cggcgcggcc gaccttgcac ggatcgagca aggcttcacc
     1081 gaggacgccg tggctcgctt cgccgacttc cccgagatgc gcggccgcga tgcagtgatg
     1141 accttcctgc gtgcacggtt cgctcgaacc aaaggctatc gcctgcgtaa gactctccac
     1201 tgcgtgatgg gcgacacact cgccaacacg tgggacgcaa gttgggagga cgcgcagacc
     1261 ggcaagcaaa tgctggggcg tggcaccgag atctggatcg tgcgcgacgg ccgcatcgcc
     1321 ctatgggatg cgaccttcaa cgcctgggag aaaggcggaa agccaacaac tcccgtggtg
     1381 tagcgctcag ccccagccca actcgcgcat cgcccacccg gcgttccaga tcttcgtcat
     1441 atggctgatc ttctcgccgg tgaactgcat gacgtagacg tagtcggtgg tcgtgctctt
     1501 gccggttggc ggacacgggc cgccctcacc cgtgtgggtg gcctggaaca cgccgtaggc
     1561 actgacgttg ctacgcgcct cgtcgacagc gaaagatttc accgtgtaac ggccatcagg
     1621 catgaaggtc agcaggccct tcatccaatc gcagtactcg cgcagtgtct tcacattggc
     1681 cagcggctcc gcctgggcgg cgaaactggc gtcgggcgtg caatacgcct ggcaaccctc
     1741 ccagcccctg ccggtctcgc aggcctcaaa gaaccgttgt gatatcgcac taatatcggc
     1801 catgtcgaag ctccctgttg gcgcaacgac aatagctcac cttggtgatt ggaccgcagc
     1861 aaaatgtcgg atcgccgcag tcacggcggt cagcgtttgc cgcgctggga ctcgacgaac
     1921 gcgcgcagga cagcgttcat gcgggtctga tagccggcac cgccgccacg gaaccagtcc
     1981 agcacgtcct ggtcgatccg cagtgagatc gcctgctttg cctgcggcca atcgaccttc
     2041 aggctgttcc agtcgggttg tttctggcgg gctgtcgtac ccgccttggc cttcgctttg
     2101 gctttcgcct tcgctggctg cttcttcttg gctgcgcgtg ccatggctgc ctcctcgtgg
     2161 ctataacgat gcctatacga gtgtatccgg ccgggggcaa gggtacgtgt tctggccaaa
     2221 accggcgggc aaaccgctac cgggcggtac cttttgccag caaccggaag cggcccaact
     2281 gcatatacgc ccgatcgcgt ccagtaacga tcggcgagac ggccaaagcc agcacaggcc
     2341 gaaggccaat aagaatcgcc tccgcgaagt tcagcgactc tgcctcggcg cgtctatgtt
     2401 gactaagcct gctgccaacc gattggcagt agccgcatgc gccctgagca caggcgtgag
     2461 caggccggac acatccgggg cagttgcgat caatccccgc ccagggccgc ctcggcagcc
     2521 cgtacggccc gcagcggcac ggacgcccgt cctactacct gcccgccgac acatgccaac
     2581 cagtccccgc cgtccggggc ggtggggcgc agctcgatca cctttgcctc atcgtccaga
     2641 gccacggcgc gatacgtgcg ccagcccgac cagaccgggc ccaaaatacg aacgacgcgg
     2701 gcgatagcct cgcccgcgtc gttgcatccg atccattcga gggtccggtg aacacccggt
     2761 ccctcctcct gccggccccg cgaccagtgt aggcgcgggg tttccactag gctgcctccg
     2821 tcatgcggtc ttcgccatga tctcgtccgc gatgttccgc ggcaccggat cgtagtggtg
     2881 gaactgcatg gtgaacgagg ccctgccctt ggtcatgctt ctcagatctg aaatatatcc
     2941 gaacatttcc ttcaggggaa catgggcgcg gacgatgacc gtcgatccgc tgctctcctg
     3001 gttctggatc atgccacgac gcctgttcag gtcgcccacc acgtcgccca cgtgatcgga
     3061 aggtgtggtc acctccacat ccatcaccgg ctccaggatg atcggcgtcg ccttcttcat
     3121 cccgtcgcgg aagcaggact tcgccgcgat ctcgaacgcc agcgccgaac tgtccacgtc
     3181 gtggtacttg ccgtccacca gcgtgtattt gaagtccacc gtggggaagc cagccagcac
     3241 gccggtctcg gcctgcaccg cgatgccctt ctcgaccgcc gggatgtatt ccttcggcac
     3301 cgagccgccg accaccttgt tctcgaacac cacgccggcg ttccgctcca gcggttcgaa
     3361 cacgatcttc acctcggcat actggccgga accgccggtc tgcttcttgt gggtgtaggt
     3421 ctcggtgtgc gg
//
LOCUS       DATGEM010000475         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1485763, whole genome shotgun sequence.
ACCESSION   DATGEM010000475 DATGEM010000000
VERSION     DATGEM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485763"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VKQ27_14885"
     CDS             complement(<1..1096)
                     /locus_tag="VKQ27_14885"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HLY90265.1"
                     /translation="MAFDEMVDGNRGVRPSWRGLLGVLAGLGRETLVERAARLDRMAA
                     EEGPPILLPGAADPWRFDPIPLPLAQAEFQQLEAGLIQRARLLDAILCDVYGEQRLLA
                     TGVVPPALVYANPGFLRPCRHVEGPPPRGLLRFYAADLIRGPDGAWRVVADRTDWADG
                     VAYALENRRRLSRVVPEVFTAQQLRALDGFVEVWADLLQRLAQDGGAGVALLTPGHED
                     PGWYGHVLLARELSCELVEGGDLTVRGERLFLKTLRGLQPIRVLLRTIAGRLVDPLEL
                     APDGTGVPGLLAAVRGTVRIVNDPGSGFAEAPALAAFLPQLARVLSSEELQLQSVDTV
                     WLGDEARLADVLREPEAWLLRPSFDAGAAPV"
     gene            1519..2154
                     /locus_tag="VKQ27_14890"
     CDS             1519..2154
                     /locus_tag="VKQ27_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90266.1"
                     /translation="MEERSTEARYRHRQSDHFNARGSLHGKPPYLAGDVESADVKIIT
                     VGSWSLPRKRIAMRAHILVTLAGLLASPGVPAFAQIIDAAISSPRTACAKQCSPPLGE
                     DPPASSGNLRLVGQTLDDAAGFALEQLIIALHNSAIVGATPIPDRVRQRLSGYASENS
                     MNRVRYKIDDSGLLNLTQVLESGGVSAVTLIDVVIFRGPSEANEVLPRIAC"
BASE COUNT          428 a          802 c          660 g          374 t
ORIGIN      
        1 caaccggcgc cgcgcccgca tcgaacgacg gccgcaacag ccacgcttcc ggctcgcgca
       61 gcacgtccgc cagccgcgct tcatcaccca gccacactgt atccacgctc tgtagctgca
      121 gctcttcact ggacaatact cgcgcgagct gaggcaggaa cgccgccagt gccggtgcct
      181 cggcaaagcc ggaaccgggg tcgttgacga tccgcaccgt gccacgcacc gcggcgagca
      241 gccccggcac gccggtgcca tccggcgcca gctccagcgg atcgaccagc cgccccgcga
      301 tggtccgcag cagcacccgg atcggctgca acccgcgcag cgtcttcagg aacagccgct
      361 cgccccgcac cgtcagatcg ccgccctcca ccagttcgca tgacagctcg cgagccagca
      421 gcacatgccc gtaccagccg gggtcctcat gcccgggcgt cagcagggca acgcccgcgc
      481 cgccatcctg agccaggcgt tgcagcagat ccgcccacac ctccacgaac ccatccaggg
      541 cccgaagctg ctgcgccgtg aacacctccg gcaccacgcg cgacagccgc cgccggttct
      601 ccagcgcata cgccacgcca tccgcccaat cggtccgatc cgcgacaacg cgccacgccc
      661 catccggccc ccggatcaga tcggctgcgt aaaaccgcaa cagcccacgc ggcggcggac
      721 cttccacatg ccggcacggc cgcaagaacc ccggattggc atagaccaga gccggcggca
      781 ccacaccggt cgccagcagc cgctgctcgc catacacatc gcacaggatc gcatcgagca
      841 gccgcgcgcg ctggatgagc ccggcctcga gctgctggaa ttccgcctgc gccagaggca
      901 gcgggatcgg atcgaaccgc cacggatcgg ctgcaccagg caacaggatg ggggggccct
      961 cctccgccgc catccgatcc agccgcgccg cacgttcgac gagcgtctcg cggcccagcc
     1021 ccgcgagcac accaagcagc ccgcgccaac tcggccggac tccccggttg ccgtccacca
     1081 tctcgtcgaa tgccatgggc gtgaagctcg ctggttgcgt cggcagtggc aagatgccgc
     1141 gcgtaattac tcagcctcct tccattgcag gctcgggccg cgaggcggac attgtcccat
     1201 tacttgaggc gttcccaggg cccgcctctg aatggacgcg gaaggtctgc gtccgtgaag
     1261 agtttcgggc gagctgcggg tggaagcgga cacgggtccg acgttgcgcg caaaccgcac
     1321 gaataaccgc tccaatccac tatgtttcgc gcgaggcggc gctccgagcg ggcaggcctg
     1381 aaacgagcga cggcaatgga gcggacacct cggccttggt cgcccagttc atcatccgag
     1441 ccggacgccg agtatccgct gccgacgcgc attttttgaa gctacttctg cgcatgatga
     1501 acttggcatg tgagagcaat ggaagagcga tccacagagg ctcgctaccg ccaccggcag
     1561 tctgaccact ttaacgctcg cggctcgttg catggtaagc cgccgtatct cgccggagat
     1621 gtcgaaagtg cggacgtcaa gatcattacg gtcggctcct ggtcccttcc aagaaaacga
     1681 attgccatga gggcccatat cctggtcact cttgcgggcc ttctcgcaag tcctggcgtt
     1741 cctgcgttcg cgcagatcat tgacgccgca atatcctcgc cgcgaaccgc atgtgcaaaa
     1801 caatgcagtc cccctttggg cgaggatcca cctgcttcct cgggtaacct gcgcttggtc
     1861 ggccagacgc tggacgacgc ggcaggtttc gcgctcgagc agctgatcat cgcacttcat
     1921 aactcagcca tagtcggtgc gacgccaatt cctgaccggg tcagacaacg gctgtcgggt
     1981 tacgccagcg agaactcgat gaaccgggtc cgatataaaa ttgacgacag tggcttgctt
     2041 aatctcacgc aagtgctgga atcggggggc gtctctgctg tcacgctgat cgatgtggtt
     2101 atttttcgtg ggccatctga agcaaacgag gttcttccgc gaattgcgtg ctgatgggac
     2161 ttgtacgagg cgaccgaatc agaaagagac ctggactcac gtccatggtg tctactcgtg
     2221 tcctctcgct tgctgtcatt cgttcccagt gtccttcagg taac
//
LOCUS       DATGEM010000476         6472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_809970, whole genome shotgun sequence.
ACCESSION   DATGEM010000476 DATGEM010000000
VERSION     DATGEM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809970"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VKQ27_14895"
     CDS             complement(<1..757)
                     /locus_tag="VKQ27_14895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90267.1"
                     /translation="MSSTTAPGGTGASFGMLNRNRTIAGIGFSRWMVPPAALCVHLCI
                     GQAYAFSVFNLPMTRLIGITQSAPGDWKLTELGWIFSIAIFFLGVSAALLGRWVEEGG
                     PRRAMFTAGLCWAGGFLLSAVGVYMHTIWVVYLGYGVLGGFGLGIGYISPVSTLIKWF
                     PDRPGMATGMAIMGFGGGAFIASPLSVWLMHQFSTSTHVGVAETFIVLGVIYFCFMMV
                     GAAIVRLPAAGWMPAGYVPPAQPRKLITTKDVFV"
     gene            complement(816..1007)
                     /locus_tag="VKQ27_14900"
     CDS             complement(816..1007)
                     /locus_tag="VKQ27_14900"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HLY90268.1"
                     /translation="MANQIGKFFAHQPHDKAVAAITDHLQKFWDPRMRGTITQHLADV
                     QLDPSVREAVEQLRESRQE"
     gene            complement(1021..1845)
                     /gene="fdhD"
                     /locus_tag="VKQ27_14905"
     CDS             complement(1021..1845)
                     /gene="fdhD"
                     /locus_tag="VKQ27_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLY90269.1"
                     /translation="MPQPSSARSVARIAYSGDAVARGARSIPEETPIAFTYNRSAYAV
                     MMATPADLEDFALGFSLTEGVVRSADDIQELEVVPSEQGIELRMWLAGDRLDAIAQRQ
                     RRLAGPSGCGLCGLESLADAIRALPRVTAATRFDAAAIHTAMTALPAAQKLNQQTHAV
                     HAAAFWRPQRGLVALREDVGRHNALDKLAGALAKDGVAASEGLLLLSSRISVEMVQKS
                     AMLGAAVVVAVSAPTALAVRLAEEAGITLIGVARADGFEVFTHSERIATQQVQNVA"
     gene            complement(1845..4682)
                     /gene="fdhF"
                     /locus_tag="VKQ27_14910"
     CDS             complement(1845..4682)
                     /gene="fdhF"
                     /locus_tag="VKQ27_14910"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HLY90270.1"
                     /translation="MPLIQEIDYGTPAVTAEKAVTLTIDGHDVTVPEGTSIMRAAMDA
                     GIKVPKLCATDSLNSFGSCRLCLVEIEGRPGTPASCTTLVAPGMKVHTSGEKVRRIRR
                     GVMELYISDHPLDCLTCTANGNCELQDAAGEVGLRDVRYGYDGANHLSAPIDASNPYF
                     QFEPSKCIVCSRCVRACEEVQGTFALTVQGRGFASKIAAGMDEPFLTSECVSCGACVQ
                     ACPTATLIEKTVVDIGQPEHSVVTTCAYCGVGCNFKAEMRGDTVVRMVPWKDGKANHG
                     HSCVKGRFAWGYAQHQDRILSPMMREKITDPWREVSWEEALGRVASEFRRIQAEAGKD
                     AIGVITSSRCTDEETYLVQKLARQVFRNNNTDTCARVCHSPTGYGLSRTFGTSAGTQD
                     FDSVDLADVIVVIGANPTDGHPVFASRMKRRLREGAKLIVIDPRRIDLVRMPHVEASH
                     HLALRPGTNVAVLTALAHVIVTEGLVNETFVRERCDAEAFADWAAFVSEQRNSPEEVE
                     NISGVPAESIRAAARLYASAGNGAIYYGLGVTEHSQGTTAVMGLANLAMATGNIGRRG
                     VGVNPLRGQNNVQGSCDMGSFPHELPGYRHISIPEVRAIYEAEWGMTLDPEPGLRIPN
                     MIDAALDGSFKGIYIQGEDIVQSDPDTKHMTAGLAAMECVVVHDLFLNETANYAHVFL
                     PGSTFLEKDGTFINAERRINLVRKVMAPRAGLADWEVTQEIARAMGCDWNYTHPSQIM
                     DEIARTTPTFAGVSFDRLAQGSLQWPVNDKAPEGTPIMHIGQFVRGKGNFLITEYVAT
                     DEKVGPRFPLLLTTGRTLSQYNVGAQTRRTENVVWHHEDVLEIHPQDAEDRGVRDGDL
                     VRLASRSGETTLRARITDRVAPGVVYTTFHHPATQANVVTSDYSDWATNCPEYKVTAV
                     QVSPSNGPSEWQEEYRELTQRSRRIEAVPAK"
     gene            complement(4819..6336)
                     /locus_tag="VKQ27_14915"
     CDS             complement(4819..6336)
                     /locus_tag="VKQ27_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HLY90271.1"
                     /translation="MTTRVYVPRDAAALALGADAVASLLAQVGDVQIVRTGSRGLFWL
                     EPMVEVETAEGRVAYGPVTVADISGLLGAGMLVGGPHPLRLGRPEDIPYLAKQTRLTF
                     ARCGIVDPLSLDDYRAHRGLRGLARAREIGPAATIELVTQSGLRGRGGAGFPTGIKWK
                     TAADATGERKFIVCNADEGDSGTFADRMLMEGDPFVLIEGMAIAGFAVGASKGYVYTR
                     SEYPHAIATFDAALEIARRDGLLGAHFDIEQRVGAGAYVCGEETSLLDSLEGKRGQVR
                     AKPPLPAHQGLFGAPTVINNVISLASVPVILERGAEFYRDIGMGRSRGTMPIQLAGNV
                     KYGGLFECGFGITLGELVNDVGGGTLSGRPVRAVQVGGPLGAYFPPSLFDTPFDYEAF
                     AAKDGLIGHGGIVVFDDTVDMAKQARFAMEFCAAESCGKCTPCRIGSVRGMEVIDRVV
                     AGEKPAANLELLRDLCHTLKFGSLCALGGFIPYPVMSALEYWPEDFAPARREAAE"
     gene            complement(6333..6443)
                     /locus_tag="VKQ27_14920"
     CDS             complement(6333..6443)
                     /locus_tag="VKQ27_14920"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90272.1"
                     /translation="MFCLGLCAIGPSAMLDGAPLGRLDNAKIDSMLERAR"
BASE COUNT         1208 a         2190 c         1983 g         1091 t
ORIGIN      
        1 agacaaacac gtccttggtg gtgatcaatt tcctcggctg cgccggaggc acgtatcctg
       61 ccggcatcca acccgcggcc ggcagccgca cgattgccgc accgaccatc atgaagcaaa
      121 aataaatgac accgagcacg atgaaggtct cggcgacgcc gacatgcgtg ctggtcgaaa
      181 actgatgcat cagccacacc gacaaaggcg aggcgatgaa cgcaccgccg ccaaagccca
      241 tgatcgccat gccggtcgcc atgcccgggc gatcggggaa ccatttgatg agcgtcgaga
      301 ccggcgagat atagccgatg cccaggccga agccgccgag cacgccatat cccagataca
      361 ccacccagat ggtgtgcata tacacgccga cagccgacag caggaacccg cccgcccagc
      421 acagtccagc ggtgaacatg gccctgcgcg ggccgccttc ctccacccat cggccaagca
      481 aggcagcgga cacgcccagg aagaagatgg caatgctgaa gatccaccca agctcggtga
      541 gcttccaatc gcccggggcc gattgcgtga taccaataag ccgcgtcatc ggcaggttga
      601 acacgctgaa ggcataagcc tgcccgatgc agagatgcac gcacagtgca gcgggcggta
      661 ccatccagcg actgaagcca attcccgcaa tcgtgcgatt gcgattgagc atgccaaacg
      721 aggcgccagt gccccctggc gcagtcgtcg aactcatgga aggcctcccg aaacgtcttt
      781 tgatttttga tagtcccagg ggatcctgga tcgaattatt cttgccgcga ttcgcggagt
      841 tgttcaaccg cctcgcggac cgaaggatca agctgcacat cagccaaatg ctgagtgatg
      901 gtaccccgca tccgcggatc ccagaatttc tgcaaatggt cggtgatcgc ggccaccgcc
      961 ttgtcgtgcg gctggtgcgc aaagaacttg ccaatctgat tggccatgta ggcaagcttc
     1021 tcaggcgaca ttctggacct gttgcgtagc aatgcgttcg gaatgagtga aaacttcaaa
     1081 gccgtcggca cgggcaacgc caatgagcgt gatgccggcc tcttccgcca gacgaaccgc
     1141 gagcgcagtc ggtgcggaaa ccgcgaccac gaccgccgct ccaagcatcg ctgacttctg
     1201 aaccatctcc accgagatgc ggctcgacag cagcagcaat ccttcgctgg cagccacgcc
     1261 atctttcgcc agcgcgcccg ccagtttgtc gagggcattg tggcgtccga catcttcgcg
     1321 caacgcgacc aggccacgtt gcgggcgcca gaaggcggcc gcgtgtaccg catgtgtttg
     1381 ttgattcagc ttctgcgcgg cgggcagcgc tgtcatcgcc gtgtggatcg ccgcggcatc
     1441 aaagcgcgtg gcggcggtga cgcgcggcaa cgcgcgaatg gcatcggcga ggctctccag
     1501 cccgcacagg ccacagccgc tcggcccggc aaggcggcgc tggcgttgcg cgatcgcgtc
     1561 aaggcgatcg cccgcaagcc acatgcgaag ctcgatgccc tgttccgagg gaacgacctc
     1621 cagttcctgg atatcgtcgg cagagcgcac cacaccttcg gtcaggctga agcccagagc
     1681 gaaatcctcg aggtcggccg gcgtcgccat catcaccgca tatgcactgc ggttatacgt
     1741 gaaggcgatc ggcgtctctt ccgggatcga tcgcgcgccg cgcgctaccg catcgcctga
     1801 ataggcgatg cgcgcgacac tgcgggccga ggaaggctgc ggcattactt tgccggcacg
     1861 gcctcgatgc gccggctccg ctgcgtcagc tcgcgatact cttcctgcca ttcgctcgga
     1921 ccgttggatg gcgagacctg caccgcggtc accttgtatt ccgggcagtt ggtcgcccag
     1981 tcggaatagt cgctggtgac cacgttggcc tgcgtggccg gatggtggaa cgtcgtatac
     2041 acaacgcccg gcgccacccg atcggtgatg cgcgcccgga gcgtggtttc gccggaacgg
     2101 cttgccagcc gcacaaggtc gccatcacgt acgccgcggt cttcggcatc ctgcggatgg
     2161 atctccagca cgtcttcgtg gtgccacacc acgttctcgg tgcgccgcgt ctgcgcgccc
     2221 acattgtatt gcgacagggt gcggccggtg gtcagcagca gcgggaagcg cggcccgacc
     2281 ttctcgtcgg tcgccacgta ttcagtgatg aggaaattgc ccttgcctcg gacgaactgc
     2341 ccgatatgca tgatcggcgt gccctccggc gctttgtcgt tcaccggcca ctgcaacgag
     2401 ccctgcgcca gcctgtcgaa cgaaaccccg gcaaacgtcg gcgtggtgcg ggcgatctca
     2461 tccatgatct gcgacgggtg cgtgtagttc cagtcgcagc ccatcgcgcg ggcgatctcc
     2521 tgcgtcactt cccagtccgc cagccctgcg cgcggtgcca tgaccttgcg taccaggttg
     2581 atgcggcgct cggcgttgat gaacgtgccg tccttttcca aaaacgtgct gcccggcagg
     2641 aagacgtgcg catagttcgc cgtttcgttc aggaacaggt cgtgtaccac gacgcattcc
     2701 atcgccgcca ggccggcggt catgtgcttc gtgtcgggat ccgactgcac gatgtcctcg
     2761 ccctggatgt agataccctt gaacgaacca tccagcgccg cgtcgatcat gttggggatc
     2821 cgcaggcccg gctcgggatc gagcgtcatg ccccactcgg cctcgtagat cgcccgcacc
     2881 tcagggatcg agatgtggcg atagccgggc agctcgtgcg ggaagctgcc catgtcgcag
     2941 gagccctgca cgttgttctg gccacgcagc gggttcacgc cgacgccgcg gcggccgata
     3001 ttgccggtcg ccatcgccag gttcgcaagc cccatcaccg cggtggtgcc ctggctgtgc
     3061 tctgtgacac ccagcccgta gtagatcgcg ccattgcctg cgctggcgta gagccgcgcc
     3121 gccgcacgga tgctctccgc cggcacgccg ctgatattct caacttcctc cgggctgttg
     3181 cgctgctcag agacgaacgc tgcccagtcg gcgaacgcct cggcatcgca gcgctcacgc
     3241 acgaaggtct cgttgaccag gccctcggtg acgatgacat gcgccagcgc ggtcagcacc
     3301 gcgacgttgg tgcccgggcg cagcgccaga tgatgactcg cttcgacatg cggcatgcgc
     3361 accaggtcga tacggcgtgg gtcgatcacg atcagcttgg cgccttcgcg cagccggcgc
     3421 ttcatccgcg aggcgaacac cggatgccca tcggtcggat tggcgccgat aaccacgatc
     3481 acgtcggcca gatccaccga atcgaaatcc tgggtgccgg ccgaggtgcc gaaggtgcgg
     3541 ctaagcccgt agccggttgg cgaatggcag acacgggcgc aggtatctgt gttgttgttg
     3601 cggaacacct ggcgcgccag cttctgcacc agatacgtct cttcatcggt gcagcgcgac
     3661 gacgtgatga cgccgatggc gtccttccca gcctctgcct ggatgcgcct gaactccgag
     3721 gcgacgcggc ccagcgcttc ttcccacgac acttcgcgcc acggatcggt gatcttctcg
     3781 cgcatcatcg ggctcaggat acggtcctgg tgctgcgcat agccccaggc gaagcgcccc
     3841 ttcacgcagg aatgcccgtg gttcgccttg ccgtctttcc agggcaccat gcgcacgacg
     3901 gtatcgccac gcatctccgc cttgaagttg cagccgacgc cgcaataggc gcaggtggtc
     3961 accacggaat gctctggctg gccgatatcc accacggtct tttcgatcag cgtggcggtc
     4021 gggcaggcct gcacgcaggc gccgcacgag acgcactcgc tggtcaggaa cggttcgtcc
     4081 atgccggcgg cgatcttcga ggcgaagccg cggccttgca cggtgagcgc gaacgtgccc
     4141 tgcacttcct cgcaagcgcg cacgcagcgc gagcagacga tgcatttgga aggttcgaac
     4201 tggaaatacg ggttggaggc gtcgatcggc gcgctgaggt ggttggcgcc gtcatagcca
     4261 taacggacgt cgcgcagccc gacttcaccg gcggcatcct gaagctcgca attgccgttg
     4321 gcagtgcagg tcaggcaatc gagcggatgg tcggagatat acaactccat cacgccgcgg
     4381 cggatgcggc gcaccttctc gcccgacgta tgcaccttca tgccgggtgc aacgagcgtg
     4441 gtgcatgagg caggggtgcc cgggcgaccc tcgatctcaa cgagacacag tcggcaactg
     4501 ccgaaactgt tcagcgagtc cgtggcgcag agcttcggca ccttgatgcc ggcgtccatc
     4561 gccgcacgca tgatcgaggt gccctcgggc accgtcacat catggccgtc gatcgtcagc
     4621 gtcacggcct tctcggcggt gaccgccggc gtgccgtagt cgatttcctg gatcaggggc
     4681 atggcagcat ccctagcaag ttgtcactgc ggagccgaag cgacgaagca atcccgaacg
     4741 tgcgatgcgc gcacagccgg gtcgagacag cttcgccgtt gcgcggctcg cagcgacaga
     4801 aggtagaaca gatgggcgtc attccgcggc ctcacggcgc gcgggagcaa aatcttccgg
     4861 ccagtattcg agtgcgctca tcaccgggta cgggatgaac ccaccaagcg cgcacagcga
     4921 gccgaacttc aacgtgtggc aaagatcgcg cagcaattcg aggttggctg ccggcttctc
     4981 accggcgacg acacggtcga tcacctccat gccgcgcacc gaaccgatgc ggcacggcgt
     5041 gcacttgcca caggactccg cagcacagaa ctccatcgcg aaacgcgcct gcttcgccat
     5101 atccacggta tcgtcgaaca ccacgatgcc gccgtgaccg atcagcccat ccttcgcggc
     5161 aaatgcctca tagtcaaacg gcgtatcgaa cagcgatggc gggaaatacg cccccagcgg
     5221 cccgccaacc tgcactgcac gcaccggacg gccagataac gtgccaccac cgacatcgtt
     5281 caccagctca cctagcgtga tgccgaagcc gcactcgaac aacccgccat acttcacgtt
     5341 gccggcaagc tggatcggca tggtgccacg tgagcggccc atgccgatgt cgcgatagaa
     5401 ctccgcgccg cgttccagga tgacaggcac gctcgcgagc gagatcacgt tgttgatcac
     5461 cgtcggcgcg ccgaacagcc cctggtgcgc cggcagtggc ggcttggcgc gcacctggcc
     5521 gcgtttgcct tccaggctgt ccaacagcga cgtctcttcg ccgcagacat acgcgccggc
     5581 cccgacacgc tgctcgatgt cgaaatgcgc gccgagcagc ccgtcgcgcc gcgcgatctc
     5641 cagcgcagca tcgaatgtgg caatcgcgtg cggatattcg gaacgcgtat agacataccc
     5701 cttgctcgcg ccgacagcga agccggcgat cgccatgcct tcgatcagca cgaacggatc
     5761 gccttccatc agcatgcgat cggcaaatgt gccggaatcg ccttcatcgg cgttgcaaac
     5821 gatgaacttc cgttcacctg ttgcgtcggc ggcggtcttc catttgatcc cggtcgggaa
     5881 ccccgcgccg ccccggccgc gcaaccccga ttgcgtgacc agctcgatcg ttgccgccgg
     5941 accgatttcc cgcgcgcgcg ccaggccgcg cagcccgcga tgggcgcgat aatcgtccaa
     6001 agacagcgga tcaacgatgc cgcagcgggc gaaggtaagc cgggtctgct tcgccaggta
     6061 cggaatgtcc tccggccgcc cgaggcgcag cggatgcggg ccacccacca gcatgccggc
     6121 acccagcaga ccggagatgt cggcgaccgt caccggaccg taggcaacgc ggccctcggc
     6181 ggtctcgacc tcgaccatcg gctcgagcca gaacaggccc ctcgagccgg tgcgcactat
     6241 ctgaacgtca cctacctgcg ccaacagcga cgccacggcg tcggcaccca aggcaagcgc
     6301 tgcggcatca cgcggaacgt agacacgcgt ggtcatctgg cccgctccag catcgaatcg
     6361 attttcgcgt tgtccagccg ccccagcggc gctccatcca gcatggcgga cgggccaatc
     6421 gcgcacaggc caagacagaa caccggttcc agcgtgaccg caccgtccgc cg
//
LOCUS       DATGEM010000477        19769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1083876, whole genome shotgun sequence.
ACCESSION   DATGEM010000477 DATGEM010000000
VERSION     DATGEM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 19769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19769
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083876"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..774)
                     /locus_tag="VKQ27_14925"
     CDS             complement(1..774)
                     /locus_tag="VKQ27_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY90273.1"
                     /translation="MAWNDTAITIAGTKMHLSRGGSGRPVLILHHDIGTPDRLPFYDT
                     LAQSFDVLVPHHPGWGKSERPQWMRSVRDIAAMHSWLLAELGVHDVSLVGLGFGGWVA
                     AEMASQAPAAYRKLILVGAMGINPPGGYIADQAIVSYIDYPKAGFHDETAFTRVYGDV
                     STDQLEQWDICREMVFRTAWKPYMYSQTLPHLLGGIRMPALVVWGDDDKHVPKSAGEV
                     YAKSIPNARLETVAACGHYVDMEKPDELAKLVTTFVNAN"
     gene            852..1616
                     /locus_tag="VKQ27_14930"
     CDS             852..1616
                     /locus_tag="VKQ27_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY90274.1"
                     /translation="MAALSGKVAIVTGASRGIGEAAALALGEAGASVVLAARSAQQLE
                     ANVRKIVAAGGKAFALECDVSDYAACERLVRETAARFGPPDILVNNAGVIEPISMVAT
                     ADPREWARSIEINLTGAYYAIRAVLPGMLERGRGDIINVSSGAAHRPLEGWSAYCSGK
                     AGLAMLTRAIDLEHSAGGIRVFGFQPGTTDTDMQVAIRASGINMISQIPREKLTPVAL
                     PAKAIVYLCTKEADDYKGKDFSLNDAEFRRRALGDG"
     gene            1632..3311
                     /gene="ettA"
                     /locus_tag="VKQ27_14935"
     CDS             1632..3311
                     /gene="ettA"
                     /locus_tag="VKQ27_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HLY90275.1"
                     /translation="MASYQYVYVMKDLTKAFPGGREVFKGITLAFLPGVKIGVLGVNG
                     AGKSTLLKIMAGIETEYGGEAWAAEGVRVGYLEQEPQLDPEKTVSENVEEAFADLKAA
                     LNRFNEVSMKFAEPMSDDEMNDLLAEQADLQTKIDAEEGWDLDRRVEIALDALRCPPA
                     DANVTKLSGGERRRVALCRLLLEKPDLLLLDEPTNHLDAESVAWLEHTLETYAGTVMI
                     VTHDRYFLDNVTGWILEIERGRGYPFEGNYSSWLEQKRKRLQQEEKEESARQRALAME
                     QEWIAASPRARQAKNQARIKRYEEMLAKSQEQATGVAEIVIPPGPRLGNVVIEAEGLR
                     KGYGNNLLIDDLSFKLPPGGIVGVIGPNGAGKTTLFRMIIGQEQPDGGSLRVGETVKL
                     GYVDQSRDALNADKTVWQEISNGEEVIYLGKRAVQSRAYVAAFNFKGSDQQKKVGILS
                     GGERNRVHLAKMLKTEHNVILLDEPTNDLDVDTLRALEDALIEFAGCAVIISHDRWFL
                     DRLATHILAFEDESHVEWFEGNFEAYEEDKRRRLGADATEPHRIKYRPLTR"
     gene            complement(3312..4733)
                     /locus_tag="VKQ27_14940"
     CDS             complement(3312..4733)
                     /locus_tag="VKQ27_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLY90276.1"
                     /translation="MDIRHVVVLMLENRSFDCMLGRLYPKSAAFDGINGSEINVWHKS
                     DGTGQEFQAWTDQALTARTVLIPDPDPGELFTDIQMQLRGLAGDGKTLNPSFTMGGFV
                     DNYMRQPATTPAPDPRAVMHCFTPDQVPVISQLALAFGVSDRWHASAPCQTWPNRFFA
                     HTGTAAGYVNNSPTHFPYRMPTVFNRLSSVRQSWQVYFHDIPQSATLAELWGHTLTNF
                     SHFEHDFAHDAANGKLPAYSFIEPRYFTDTVLNKIPNDSHPPHNVAYGEQLIAEVYNA
                     LRAGPGWNQTLLMITCDEHGGCYDHVPPPPATPPDALRPDGFDFSYFGVRVPAVIVSP
                     YVKAGSVIRPPGETPFDHTSIIATLRTLFNFAPLTQRDAAAPDLLESLQQEPLNDGPA
                     SITAPAPPPAPTEVAGVAAKPPNDLQASLSTLALQLPTVGADVGAHIQRLINVVDVAP
                     GHATVDEAAAAVSGHMQAFLGQR"
     gene            4822..5571
                     /locus_tag="VKQ27_14945"
     CDS             4822..5571
                     /locus_tag="VKQ27_14945"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLY90277.1"
                     /translation="MDLGAAPTTTTDADWQARVDLAAVYRLLAHHGWGDVIFNHAAMR
                     VPSNSRRFLIKRHEHLYTEVTASNLVEVSMDDDLDERSGVNRPGFTLHSGVLRGRTDA
                     NCSVHVHPDEGVAISGLKHGLRPVSQSAMRFYRRVGYHDYEGLTDSFDERERIVTALG
                     KNRALVMRHHGITTVGVTAREAFSLMKEFVRAARIQLMMESSGQPVLEIAPEICEHVA
                     AQYELHDRGRASAEWPAYLRLLDGIDPSYRE"
     gene            5632..6231
                     /locus_tag="VKQ27_14950"
     CDS             5632..6231
                     /locus_tag="VKQ27_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY90278.1"
                     /translation="MNPLWVLRTARLVLTPVGGTDLPDLQAIKGDPRVFAIMLGGVRS
                     PQQTAEELANEVAHWGAHGFGWWAVRQTISAAFSRTRSAIERSGMSRDSGSQFVGITG
                     LEERPDRRGIALRFALWPEAQGRGLAREAAAVALRFGHEQAGQRRIVAVARENNFGSR
                     IVLGGIGMIECDSFVQNGYRMVMYESVASPPAPPAGASH"
     gene            complement(6194..6811)
                     /locus_tag="VKQ27_14955"
     CDS             complement(6194..6811)
                     /locus_tag="VKQ27_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90279.1"
                     /translation="MPHRRTILVSTAASLLAAAGWPARPAHAQSALQHATSFIKDVGD
                     QLVAVVNSSQPDAQKRNQLTQIIDGAVDVDAVAKFCLGRFWRSASSQQQQNYTTLFHQ
                     VLVNNITSKLGEYRGVSFVMGKAQMRDEDAVVSTVVNRPNNPPTNVDWIVTHPVSTPK
                     IIDVVAEGTSLRLTQRQDYASYLSHNNDSIDALINAMRQQVAQAG"
     gene            complement(6830..7654)
                     /locus_tag="VKQ27_14960"
     CDS             complement(6830..7654)
                     /locus_tag="VKQ27_14960"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HLY90280.1"
                     /translation="MRVAIPRHLLLVAALLLGNLAGCAKPPPASDPDAVADYNETNDP
                     LEPTNRVVYAIDNGIDTVFFEPVARAYRFIFPQPVRNGIHNVLSNLGTPVELGNDVLS
                     AKPRRAGDTTMRFLINTTVGVVGIFDVAKGWGYPDHDSDFGITMALWGVPEGPFLFLP
                     VFGPSNPRDGIGLGVDSVMDPFTWVGQGTATRALDWSRFGINAIDQRERHLDDIENTK
                     KTALDPYATFRSLYRQHRRSLIETARNDNRATIPVWFPQSGTPDGVSYSPAGPSNQ"
     gene            7856..8806
                     /gene="metF"
                     /locus_tag="VKQ27_14965"
     CDS             7856..8806
                     /gene="metF"
                     /locus_tag="VKQ27_14965"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HLY90281.1"
                     /translation="MVDLPDEPRPTLERWLTGPRFAALPARGAAHTPPLLSFEFFPPK
                     TDALEQQLWSCIRRLEPLAPRFVSVTYGAGGSTRARTHATVTRILTETALVPAAHLTC
                     VGACQAEVDEVARQYWEAGVRHIVALRGDPPPGTAYSPHPDGYPFAVDLVAGLRRVAD
                     FEISVAAYPEMHPQAASRVSDLDNLKRKLDAGATRAITNFFFDSGVYLRFLDLCLAAG
                     ITAPIVPGIMPVTNLAGLRRMAALSGVTVPDWLGHLFQGLDEDPETRRMVAAMVTAEQ
                     VRLLQANGVDEFHFYTLNRPDLAYSIAHILGVRPGTAKRS"
     gene            8865..11051
                     /locus_tag="VKQ27_14970"
     CDS             8865..11051
                     /locus_tag="VKQ27_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLY90282.1"
                     /translation="MSDYLSRLNAEQREAVETVDGPLLVLAGAGTGKTRVLTTRFAHI
                     LLTGRAFPGQVLAVTFTNKAAREMRERVGAILGRPVEGLWLGTFHALCARMLRRHADL
                     VGLSANFTILDSDDQVRLLKQVMEGERLDTKRWVPPALMGVIQRWKDRGLTPARVTPA
                     EDTDFANGRARDLYAAYQARLRSLNAADFGDLLLHMTELMRDQPEILAQYHRMFRYVL
                     VDEYQDTNLVQYLWLRLLAQAHKNICCVGDDDQSIYSWRGAEVENILRFEHDFPGARI
                     VRLEANYRSTAPILAAASALIAHNESRLGKTLRSGRTDASGDRVSVVSLWDSDEEARM
                     VGDRIEALRRDGDPLAEMAILVRAGFQTRAFEERLITLGVPYRVIGGLRFYERQEIRD
                     AIAYMRLVVQPADDLAFERIVNVPRRGVGEVALRSMHDAARADSVPLMEAAARLIASG
                     GLKGRVREQVDALLRGFVRWRAMLERDGHVVTVATLLDESGYTEMWQHDKSPEAPGRL
                     ENLKELVRALADFETLAGFLDHVSLVMENDEAADGDRVSLMTLHGAKGLEFDTVFLPG
                     WEEGLFPNQRALDEGGVKSLEEERRLAYVGLTRARRRAIVSHAANRRIYANWQTSIPS
                     RFIEELPDADIEHTGSAALARDARVLAATTFTGQFPLVARRQRTIEAWEQPGRPPRTD
                     PIPVGTRVFHQKFGYGTVTSAEDGRLDVAFDKAGEKRVLDRFVEQV"
     gene            11048..11950
                     /locus_tag="VKQ27_14975"
     CDS             11048..11950
                     /locus_tag="VKQ27_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003628656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLY90283.1"
                     /translation="MRGPRRHATPLETVSVDVPEFALEAYEAALSTACDTVGFFHDQD
                     TGDWRVEGVKPVGSGEAELAAALTLAAALSGVAASLSRAGTPAEGWLARTRAAFPEQY
                     VGRRFSMRGTHLTGARCAGRITLILDAGLAFGSGEHGSTRGCLRALEQIAYRRPRRIL
                     DLGTGSGILAMAAVRLLHRPVLATDIEPWSVRVGRDNARRNQLDPMLRLRLADGWRHR
                     VVRTAAPYDLVFANILARPLCLMAVGLALHLAPGGTAILSGLLQTQARAVETAHRRQG
                     LRLEGRIQEGPWTTLILRRAGETQ"
     gene            complement(11944..12252)
                     /locus_tag="VKQ27_14980"
     CDS             complement(11944..12252)
                     /locus_tag="VKQ27_14980"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HLY90284.1"
                     /translation="MNAPIAKDQFEFSLGNLSYIGPRYEEPQTARVNSPSGEAKSWFG
                     RLVSAIAEWRQHMVVLQEMQTMSDRELSDIGLSRSDISRVFDPSFAKDHARGRDYIAY
                     "
     gene            12438..14237
                     /locus_tag="VKQ27_14985"
     CDS             12438..14237
                     /locus_tag="VKQ27_14985"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HLY90285.1"
                     /translation="MRSNVTTEPGERLKKLRDELKRQQLDGFIVPRADEHLGEYVPAA
                     AERLAWLTGFTGSAGLAIVLADKAAVFTDGRYVLQLAEQTDPALWERRHVTEEPPPGW
                     LNANAPAGATIGYDPWLFAEEGVARYTDAGLTMRPVERNPVDVVWTDRPPPPLVPAEP
                     YPLEYAGRSAAEKREQIAGLLREVRQDAAVISDPASLAWLLNIRGGDVQFTPFALGFA
                     IAHADGGTELFMHPAKLPERTRAWLGNAVSVAGREALAPALAKLAGKRVRVDGAGSPV
                     WFAQTLRAAGASVVSAPDPCLLPKACKNSVEQQGTRNAHARDAVAVCRFLHFLAETGP
                     RGSETEISAAARLLALRSEVEGFRGESFPAISGAGEHGAIIHYRVTEESNRPIRPNEV
                     YLIDSGAQFPDGTTDITRTVWTGPGAPPQPLREQVTRVMQGHIAIATLVFPRGVCGAH
                     LDAFARRALWQVGLDYDHGTGHGVGSYLSVHEGPVSLSRLARPVPIEAGMILSDEPGY
                     YLPGAHGIRLENLLLVQPADLSGANKPFLRFETLTLAPFDRRLIDPAMLTNEELAWLN
                     AYHARVLSEVGPHLDGAAGAWLKRACAVIERSN"
     gene            14237..15064
                     /locus_tag="VKQ27_14990"
     CDS             14237..15064
                     /locus_tag="VKQ27_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY90286.1"
                     /translation="MFHRFRMGNSAATIVSDGPLTLPTASRIFAGPAKDALDAAVSAT
                     GQSTDAVRVEQNCLLLETGGKRVLFDNGLGSEKLYGPDSGKLLASLAEAGVDPASIDG
                     MVLTHAHSDHCWGTIGDDGTPNFPNATLYLSNAEIEYWQSNPPGERRQRGIAGVRKHL
                     LPLRDRMRFIRDGEEFLPGVQAWFTPGHTPGHLAFLFSGGWCLTGDVAFHDPISYAFP
                     EAESAFDVDPQLAVATRGRLLDRLVDERMQVIGYHHPWPGLGRVERVAGTFRFIRNE"
     gene            complement(15364..16578)
                     /locus_tag="VKQ27_14995"
     CDS             complement(15364..16578)
                     /locus_tag="VKQ27_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY90287.1"
                     /translation="MTTHVIVGAGQAGGWAAIGMRQAGFDGRILLIGDEPWRPYERPP
                     LSKAVLTEATEPPIAYFHAEERYAEQRIELLLGTAVAEIESAAHRIRLADGRTFDYDR
                     LLLTVGGSARHLPIHGGDKALYLRTLEDARLVRARLSNARNVICIGAGVIGLEIASSA
                     RARGCEVTVLEALPRAMGRAVSPEGATFIETLHRDAGVALHFGVIVDTIEDTPRGMRV
                     VCRDGDSYIGDLVIAGVGMQRNLALADAAGLSMEGGIVVDEFGRTSDPDIYAAGDVTA
                     FHHPLFNRRLRLESWRHAQNHGLAVGKAMCGDTTPYDDIPWFWTDQHGVNLQVAGMPA
                     DAARTIVRVNTPPKAFTAVHLAEDGCVIGVTAANNPREIRAGQAMIKARRPVDEVALA
                     SPAVPLQTLIPR"
     gene            complement(16588..16998)
                     /locus_tag="VKQ27_15000"
     CDS             complement(16588..16998)
                     /locus_tag="VKQ27_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90288.1"
                     /translation="MPGPEKWQIILGSAVLLAFATPALAQHVVLDAPSLLPPKPKAGP
                     PAPRAPTAVWPRLDPGSVLCKTEDDLSRHAANMIARVSGGEQQTADCHAIAQPVGVQI
                     VSREGPGRTEVKLAGQDGETGWTDVWLPDKPPPR"
     gene            17063..17440
                     /locus_tag="VKQ27_15005"
     CDS             17063..17440
                     /locus_tag="VKQ27_15005"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HLY90289.1"
                     /translation="MTWPVRRALAFWWERNQETEMAEAGWVRVAGVSDVAEGQVHAVR
                     VGDREIAVYHLQGGDFRATDNVCTHEYALLSDGWLEDGCIECPLHAARFDVKTGKALC
                     APAETNLQVFEVKVEGEDLLLKV"
     gene            17445..18503
                     /locus_tag="VKQ27_15010"
     CDS             17445..18503
                     /locus_tag="VKQ27_15010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90290.1"
                     /translation="MSPIVDCDLHPTVPSLAALMPYLHDMWRETVVRRGLDELNTIAY
                     PANSPLTARSDWRDERGKPATTAERLGKDALDPFGTQVAICNCLYGVQATFSEDLGAA
                     MARAVNDWIAHEWLDRDPRLRASIVVPQQNAELAVAEIERCAPDRRFVQVLLLVSGEL
                     PLGRRQNWPIYAAAERLRLPVGIHAGSTYRHPNTPVGWSSYLSEEYINQAGAFQTQLT
                     SLMAEGAFAKFPNLTFVLMESGVTWLPGYLWRLTKFWKGLRSEVPWVADPPASIVRDR
                     VRLTLQPFDGPPETATITRFMEHMGSDELLLFSTDYPHWQFDGTDAIPAGLDPALAQK
                     IMVDNPRNTYARLRETTQ"
     gene            18587..19711
                     /locus_tag="VKQ27_15015"
     CDS             18587..19711
                     /locus_tag="VKQ27_15015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90291.1"
                     /translation="MNVDVLKRTGLQTPNQSKLAIADCDIHPRPPGAGIGGVSKALYP
                     YLSKRWLEHVQTIGVRYRQPWERGSAYPKGQPQACRRDAWPKGDNPGSDLAFMAEQHL
                     DPNNVAFGVLNPLTSGQGAQDPELSAALTHATNEWQLAEWTSRDTRLKASVMTPFEDA
                     PTAAKMIEARAGNPNFAQVLLLTRTAEPLGQRRYWPIYQAAVEANLPVGIHAFGYGGS
                     ALTSGGWPSYYLEEMVGHAQCQQAVLTSLIFEGVFERFPTLKIVLIEAGFAWAASHAW
                     RMDRQFAKLRKEVPHVKRQPSEYMRSNVWFTTQPVEEPEPREHLADAIEWMGWDRVLF
                     ATDYPHWDFDDPAHALPIKMTEAQKRGIFLENARAVYGFA"
BASE COUNT         3510 a         5967 c         6721 g         3571 t
ORIGIN      
        1 tcagttcgcg ttgacgaacg tggtgacgag ttttgccagc tcgtcaggct tctccatgtc
       61 gacatagtgt ccgcaggcgg cgacggtttc cagccgggca ttggggatgc tcttggcata
      121 aacctcaccg gcgctcttcg gcacgtgctt gtcgtcgtcg ccccacacca cgagggcagg
      181 catgcggatg ccgccaagca ggtgcggcag cgtctggctg tacatgtatg gcttccaggc
      241 agtgcggaac accatctcgc ggcagatatc ccactgctcg agctggtctg tggatacatc
      301 accatatacg cgggtgaacg ccgtctcgtc gtggaaacca gccttcggat agtcgatata
      361 cgaaacgatc gcctggtcgg cgatgtagcc gccgggcggg ttgatgccca tggcaccgac
      421 aaggatcagc ttgcgatacg ctgccggcgc ctggctcgcc atttcggcgg cgacccagcc
      481 gccgaagccg aggcctacca gtgaaacgtc gtggacgccg agctcggcga gcaaccagct
      541 gtgcattgcg gcgatgtcgc gcacgctgcg catccattgc ggtcgttccg atttgcccca
      601 gccgggatgg tgcggcacca gcacatcgaa gctctgcgcc agagtgtcgt agaatggcag
      661 acggtcgggc gtaccgatgt cgtggtgcag gatcaggaca gggcggccac tgccgcctcg
      721 gctcagatgc atcttggttc cagcgatggt gatcgccgta tcgttccagg ccacgctgct
      781 tcctccagca cttgcttgat ggcgaggata ctcaccagta gcctcgcttg tccaatttgg
      841 aggcggggtc gatggcagcg ttgtctggga aggttgcaat cgtaacgggt gcgagtcgtg
      901 ggatcggcga ggctgccgca ctggcgctgg gtgaagcggg tgcgtcggtc gtgctggcgg
      961 cgcgcagcgc tcaacaactg gaagccaacg tgcgcaagat cgttgcggcg ggcggcaagg
     1021 cattcgcact tgagtgcgac gtgtcggact atgccgcgtg cgagcgtctg gtgcgcgaga
     1081 ccgctgcgcg ctttggaccg ccggatatcc tggttaacaa tgctggggtc atcgaaccga
     1141 tcagcatggt ggcgacggct gatccacggg aatgggcgcg cagcatcgag atcaacctga
     1201 ccggcgctta ttacgcgatc cgtgcagtgc tgcctgggat gctggaacgc ggacgtggcg
     1261 acatcatcaa tgtgtcatct ggcgccgcgc accggccgct ggagggttgg agcgcatatt
     1321 gctccggcaa ggccggactt gcgatgttga cgcgcgccat cgatctggag catagcgccg
     1381 gcggcattcg cgtatttggg ttccagccgg gcaccacgga caccgacatg caggtggcga
     1441 tacgggcctc gggcatcaat atgatcagcc agataccgcg cgagaaactc acaccggtgg
     1501 cgctgccggc taaagccatc gtgtatttgt gcacaaaaga ggctgacgac tacaagggca
     1561 aggatttcag tctgaacgat gcagaattcc ggcgccgcgc gctcggcgac ggatagcgag
     1621 gagaacggcc ggtggccagc taccagtacg tctacgtgat gaaagatttg acaaaggctt
     1681 ttcccggtgg gcgggaggtc ttcaagggca tcacgctggc cttcctgccc ggcgtgaaaa
     1741 tcggcgtgct tggcgtgaac ggcgccggca aatccacgct gctgaagatc atggccggga
     1801 tcgagacgga atacggcggt gaggcctggg ccgcggaggg tgtccgcgtc ggctatctgg
     1861 agcaggagcc gcagctcgat cccgagaaga cggtgagtga gaacgtcgag gaagccttcg
     1921 ccgacctaaa ggctgcgctc aacaggttca acgaagtctc gatgaagttc gccgaaccga
     1981 tgagcgacga cgagatgaac gatctgctcg ccgagcaggc cgatctccag acaaagatcg
     2041 atgcggagga aggctgggac ctcgatcgac gggtagagat cgcgctggac gcactgcgct
     2101 gcccgccggc ggacgccaac gtcaccaagc tatcgggcgg cgagcggcgg cgtgtcgcgc
     2161 tgtgccggct gctgctggaa aagcccgatc tgttgttgct ggacgagccc accaaccacc
     2221 tggacgccga gagtgttgcc tggctggagc acacgctgga gacctatgcc ggcaccgtga
     2281 tgatcgtgac gcacgatcgc tacttcctgg acaacgtcac cggctggatc ctggagatcg
     2341 agcgcgggcg cggctatccg ttcgagggca actactcctc ctggctggaa cagaagcgca
     2401 agcgcctgca gcaggaagag aaagaggaaa gcgcgcggca gcgggcgctg gcgatggaac
     2461 aggagtggat tgccgcctcg ccgcgggcac gtcaggccaa gaaccaggcc cgcatcaagc
     2521 gttacgagga gatgctggcc aagtcgcagg agcaggcgac gggggtggcg gaaatcgtca
     2581 ttccgccggg gccgcggctc ggcaatgtgg tgatcgaggc ggagggcttg cggaagggct
     2641 acggcaacaa cctgctgatc gacgatctgt cgttcaagct gccgccgggc gggatcgtcg
     2701 gggtgatcgg accgaacggc gcgggcaaga cgacgctgtt ccgcatgatc atcgggcagg
     2761 agcagccgga tggcggctca ctgcgcgtcg gcgagacggt gaagctcggc tatgtggacc
     2821 agtcgcgcga tgccctgaac gccgacaaga ccgtgtggca ggagatcagc aacggcgaag
     2881 aggtgatcta tctcggcaag cgcgccgtgc agtcgcgtgc gtatgtcgcc gccttcaact
     2941 tcaaggggtc ggatcagcag aagaaggtcg gcatcctgtc cggcggcgag cggaaccgcg
     3001 tgcacctcgc gaagatgctg aagaccgagc acaacgtgat cctgcttgat gagccgacga
     3061 acgacctgga tgtggatacg ctgcgcgcgc tggaagacgc gctaatcgag tttgccggtt
     3121 gcgcggtgat catcagccac gaccgctggt ttctcgaccg gttggcgacc cacatcctgg
     3181 cgttcgagga cgagagccac gtggagtggt tcgagggcaa tttcgaggcg tatgaagagg
     3241 acaagcgacg ccggctcggc gcggatgcca ccgagccgca ccggatcaag taccgaccgc
     3301 tgacgcgctg atcatctctg ccccaggaat gcttgcatgt ggccggacac ggccgcagct
     3361 gcctcatcga ctgtcgcgtg cccgggcgct acgtccacaa cgttgatcag ccgctggatg
     3421 tgtgcgccta cgtcggcacc cacagtgggc agttgcaggg caagggtgct caggctggcc
     3481 tgaagatcgt tgggcggctt ggcggcgacg ccggcaacct ctgtgggcgc tggtggcggg
     3541 gccggtgcgg tgattgaggc cgggccatcg tttaatggct cctgctgcaa tgactccaga
     3601 agatccgggg cggccgcatc ccgctgcgtc agcggcgcga aattgaacag cgttcgcagc
     3661 gtcgcaatga tcgaggtgtg atcgaacggt gtctcgcccg gtggccggat cacgctgccc
     3721 gccttcacgt agggtgagac gatcaccgca gggacacgga caccgaaata gctgaagtcg
     3781 aagccatccg ggcgcagcgc gtcgggtggc gttgccggcg gcggcggcac atggtcgtag
     3841 cagccaccgt gttcgtcgca ggtgatcatc agcagtgtct ggttccagcc gggtccggca
     3901 cggagtgcgt tatagacttc agcgatgagc tgctccccgt aggcgacgtt gtgcggcgga
     3961 tgcgagtcgt tggggatctt gttcaacacc gtgtcggtga agtaccgcgg ttcaatgaag
     4021 ctgtaggcgg gcaacttgcc gtttgccgca tcatgggcga agtcgtgctc gaagtgactg
     4081 aagttggtca gtgtgtgtcc ccagagttcc gcgagcgtcg ctgactgcgg aatgtcgtga
     4141 aaatacacct gccagctctg tctcaccgaa gacagacggt tgaacactgt cggcatccgg
     4201 taggggaagt gcgtcggtga attgttgaca tagccggccg cggtaccggt atgcgcgaag
     4261 aagcggttcg gccatgtctg gcacggcgcc gacgcatgcc aacggtcgga gacgccaaag
     4321 gcgagcgcca gctgactgat caccggcacc tgatcgggcg tgaaacaatg catgaccgcg
     4381 cgcggatcgg gcgccggagt ggtggccggc tggcgcatgt agttgtcgac gaagccgccc
     4441 atggtgaagc tcggattgag cgtcttgccg tcgccggcaa ggccgcgaag ctgcatctgg
     4501 atgtcggtga acagctctcc gggatccgga tcagggataa gcacggtccg cgcggtcagg
     4561 gcctggtccg tccaggcctg gaactcctgt cctgttccgt ccgatttgtg ccagacattg
     4621 atttcgctgc cattgatgcc gtcgaacgcc gcacttttcg ggtagagccg gcccagcatg
     4681 cagtcgaagg agcggttctc cagcatgaga accacgacat gcctgatgtc catcgccatc
     4741 cctcacttgc ctgttggcgg aattctatca tcattagggg aacgtacggt cgcagccacg
     4801 cataatggaa ggggttggtc gatggatctc ggagctgctc ccacgacgac gacggatgcc
     4861 gactggcagg cgcgtgtgga tctcgcagcc gtgtatcgcc tgcttgcaca tcatggatgg
     4921 ggcgatgtga tcttcaacca cgcggcgatg cgcgttcctt cgaactcacg tcgcttcctg
     4981 atcaagcgtc acgaacacct ctataccgag gtgacggcgt cgaacctggt tgaagtcagc
     5041 atggacgatg acctggacga acgctccggc gtcaaccgcc ccgggttcac gctgcatagc
     5101 ggcgtgctgc gtggcaggac tgacgcgaac tgctcggtgc acgtccatcc cgatgaaggc
     5161 gtggcaatct ccgggctgaa gcacggcctg cggccggtgt cgcaaagcgc catgcgtttc
     5221 taccggcgcg tcggctatca tgactatgaa ggcctgaccg acagcttcga cgagcgtgaa
     5281 cggattgtca ccgcgctggg caagaaccgc gcgctggtga tgcggcatca tggcatcacc
     5341 accgtggggg tcacggcgcg cgaggcgttc agtctaatga aggagtttgt ccgtgccgcg
     5401 cgtatccagt tgatgatgga atcgagtggt cagcctgtcc tggagatcgc gccggagatc
     5461 tgcgagcacg tcgcggcaca gtatgaactg catgacaggg ggcgggcatc ggctgaatgg
     5521 cccgcgtatc ttcgcctcct cgacgggatc gatcccagct atcgcgagtg acgccggctc
     5581 gatccccggg acgaacctgg ggacacgggc cagcgacggc agcaggctga cgtgaacccg
     5641 ctgtgggtcc tgcgcacggc gcggctggtg ctgacgccgg tgggaggcac ggatctgcct
     5701 gacctgcagg cgatcaaggg cgatccgcgg gtgttcgcga tcatgctggg tggcgttcgc
     5761 tcacctcagc agactgcgga agagctggcc aacgaggtgg cccattgggg agcgcacggg
     5821 tttggctggt gggcagtgcg gcagacgatt tccgctgctt tctcgcgcac gcgctcggcc
     5881 atcgagcgat cgggtatgag ccgcgactca ggcagccagt tcgtcggcat caccggcctg
     5941 gaggaacggc cagacagacg aggcatcgcg cttcgcttcg ccctctggcc ggaagcgcag
     6001 ggccgtggcc tagcgcgcga ggcagcggcg gtggcgctac ggttcggtca cgagcaggcc
     6061 ggtcagcggc gcatcgtcgc cgtggcacgc gagaataact ttggctcgcg catcgtgctg
     6121 ggcggtatcg gcatgatcga gtgcgacagc ttcgtccaga atggctatcg catggtcatg
     6181 tatgagagtg tcgctagccc gcctgcgcca cctgctggcg catcgcattg atcaacgcgt
     6241 cgatgctgtc gttgttgtgg ctgaggtagg acgcatagtc ctgccgctgg gtcaggcgca
     6301 gtgaggtacc ctcggccaca acatcgatga ttttcggcgt gctgacgggg tgggtgacga
     6361 tccaatccac gttggttggc gggttgttcg ggcggttgac caccgtggag accaccgcgt
     6421 cctcgtcacg catctgggcc ttgcccatga cgaagctgac gccgcgatat tcgcccagct
     6481 tgctggtgat gttgttcacc agcacctggt ggaacagcgt cgtataattc tgctgctgct
     6541 gggacgacgc gctgcgccag aaccggccca ggcagaattt tgccacggcg tcgacgtcca
     6601 cggcgccatc gatgatctgg gtaagctgat tgcgcttctg tgcatcgggc tgggagctgt
     6661 tcaccaccgc gacgagctgg tcgccgacat ccttgatgaa gctggtcgcg tgctgcaggg
     6721 ccgactgcgc atgcgcggga cgcgctggcc agccggcagc cgcaagcagc gatgctgcgg
     6781 tagatacgag gattgtgcgg cgatgcggca tggcgtgctc aggcctcaat cattggttcg
     6841 acggaccggc cggcgagtaa ctgacgccgt ctggtgtccc cgactgtggg aaccataccg
     6901 gaattgtcgc ccggttgtcg ttcctcgcgg tctcgatcag cgaccgtcgg tgctggcggt
     6961 aaaggctacg gaacgtggca tagggatcca gtgctgtttt cttggtgttc tctatgtcat
     7021 ccagatggcg ctcgcgctgg tcaatggcat ttataccgaa acgtgaccaa tcgagggccc
     7081 gggtggcggt gccctgtccg acccaggtga aaggatccat cacgctgtcg acaccgagac
     7141 cgattccgtc gcggggattg gacggaccga agaccggcag gaacagaaat ggcccttccg
     7201 ggacacccca taatgccatg gtgatgccaa agtcgctgtc gtgatcagga tagccccagc
     7261 ccttggccac gtcgaagatg ccgaccacgc ccacggttgt gttgatcaag aaacgcatcg
     7321 tggtatcgcc cgcgcgccgt ggtttggcgg acagcacgtc gttgccaagc tcaaccggcg
     7381 tgccaagatt gctcagcacg ttgtgaatgc cgtttcggac aggctggggg aagatgaagc
     7441 gataggcgcg cgccaccggc tcgaagaata cggtgtctat gccattatca atggcataga
     7501 caacgcgatt ggtgggctcc agcggatcat ttgtctcatt gtagtcggca actgcatcgg
     7561 ggtcgctagc cggcggcggc ttggcgcagc cagcaagatt gccgagcaac agggcagcga
     7621 ccagcagaag atgacgaggg atggcaacgc gcatcaggtt cctcggacga agcacacaaa
     7681 ttggtctcac tgccgaacca gggcggcggg gcagcttatg gtagcccggt agccaaaggg
     7741 aacaggaggc attctcccgt gtggcaactt agggggtggg cgccccccgt gcaatgtcgc
     7801 cgacgtacgc cagatcggcc ttgcaatcag gccggaaaca ttgcgaagag acgccatggt
     7861 tgatctcccg gatgagccgc gcccgacgct ggaacgctgg ctgaccggcc cgcgtttcgc
     7921 tgccttgccg gcccgcgggg cggcccatac gcctccgctg ctttcatttg agttttttcc
     7981 gccgaaaaca gacgcactgg agcagcagct ctggtcctgt atccggcggc tggagccgct
     8041 ggcgccccgt tttgtgtcgg tgacctatgg tgccggcggc agcacccggg cgcgcaccca
     8101 tgcgacagtg acccgcattc tgacggagac cgcgctggta cccgcggcac acctcacctg
     8161 cgtgggtgcc tgccaggcgg aggtggacga ggtcgcgcgg cagtattggg aggcgggtgt
     8221 gcggcatatc gtggcgctgc gcggcgaccc gccccctggg acggcatatt cgccccatcc
     8281 cgacgggtat cccttcgccg tcgacctggt agccggactg cggcgggtcg ccgacttcga
     8341 aatctcggtg gcggcctatc ccgagatgca tccgcaggct gcgtcgcggg tgagcgatct
     8401 ggataacctg aagcgaaagc tggatgcggg ggcgacgcgg gcgatcacca acttcttttt
     8461 cgatagcggg gtctacctgc ggttcctgga cctgtgtctg gcggctggaa tcacggcacc
     8521 gatcgtgccg ggcatcatgc cggtcacaaa cctggcagga ctgcgccgga tggcggcgct
     8581 gtctggcgta acagtgccgg attggcttgg acatctgttc caagggctcg atgaggatcc
     8641 cgagacgcgg cggatggttg cggcgatggt cacggctgag caagtacggt tactgcaggc
     8701 aaacggggta gacgagttcc acttctatac gctgaaccgt ccagatctgg cgtactcgat
     8761 cgcccatatc cttggcgtgc ggccaggaac ggcaaaacgc agctaagatc caagaacgtt
     8821 gtgggctggc ttcgaccacg gccataccga tatccttgcc ggcaatgtct gactatctct
     8881 cccgtctgaa cgccgagcag cgtgaagctg tcgaaaccgt cgacggccca ttgctggttt
     8941 tggcaggcgc agggaccgga aagacgcgcg tcctcaccac ccggtttgcc cacatcctgc
     9001 tgacgggccg cgccttccca gggcaggtcc ttgcagtaac cttcacaaat aaagccgcgc
     9061 gcgagatgcg cgagcgggtg ggcgcaatcc tggggcggcc cgttgaaggg ttgtggctgg
     9121 gcactttcca cgccctgtgc gcccggatgt tgcggcggca tgccgatctg gtcggcctgt
     9181 cggccaattt cacgatcctc gattccgatg atcaggtccg actgctgaaa caggtgatgg
     9241 aaggcgaacg gctcgacacg aagcgctggg ttccccctgc gctgatgggg gtgatccaac
     9301 gctggaagga ccggggtctg acgccggcaa gggtcacgcc cgctgaagac accgactttg
     9361 ccaacggtcg cgcgcgagat ctgtacgcgg cctatcaggc ccggctgcgc tcattgaacg
     9421 cagccgattt tggcgacctc ctgctgcaca tgaccgagtt gatgcgggat caaccggaaa
     9481 tcctggcgca gtaccatcga atgttccgtt acgtcctggt cgacgaatat caggacacca
     9541 atctggtgca gtatctgtgg ctccgtctgc tcgctcaggc acacaagaac atctgctgcg
     9601 ttggcgatga cgaccagtcg atttattcgt ggcgcggcgc ggaggttgag aacatcctgc
     9661 gcttcgagca cgattttccc ggcgccagga tcgtgcggct ggaggccaat tatcgttcca
     9721 ctgcgccgat acttgccgcc gcctcggcgc ttatcgcgca caatgagagc cggctgggca
     9781 agacactccg ttcggggcgt accgatgcca gcggcgaccg ggtgtcggtg gtcagccttt
     9841 gggactcgga cgaggaagca cgcatggtgg gcgaccggat cgaggcgctc cgccgtgacg
     9901 gcgatccgtt ggccgaaatg gccatcctgg ttcgtgctgg cttccagacg cgcgccttcg
     9961 aggagcggct gatcacgctg ggcgtgccct accgggtcat tggcggtctc cgtttctatg
    10021 agcggcagga aatccgcgat gccatcgcgt atatgcgcct ggtcgtgcag ccggccgacg
    10081 acctggcatt cgagcgtatc gtcaacgtgc cgcggcgcgg tgtcggcgaa gtggcactgc
    10141 ggagcatgca tgatgcggcg cgcgcggaca gtgtgccgct gatggaggcg gcggcgcggc
    10201 tgatcgcgag cggcgggctg aaagggcggg tgcgtgagca ggtcgatgcg ctgctgcgcg
    10261 gttttgtccg ctggcgggca atgctcgaaa gggacggcca cgttgtgacg gtggccactc
    10321 tgctggacga aagcggctac acagagatgt ggcagcacga caaatcgccc gaggcgcctg
    10381 gtcggctgga gaatcttaag gagttggtcc gcgcgctggc cgacttcgag acgctggctg
    10441 gattcctcga ccacgtatcg ctggtgatgg agaacgacga agcggcggac ggcgaccggg
    10501 tcagcctgat gacgctgcat ggcgccaaag ggctggagtt cgatacagta ttcctgcctg
    10561 gctgggagga aggtctgttc ccgaaccagc gggcgctcga cgaaggcgga gtgaagtcgc
    10621 tggaggagga gcgacggctt gcctatgtcg gcctgacccg agcgcgccga cgggcgatcg
    10681 tatcccatgc ggcgaaccgc cggatctatg cgaactggca gaccagcatc ccgagccggt
    10741 tcatcgagga actgccggat gccgatatcg agcatactgg ttcggcagcg ctggcgcgcg
    10801 acgcacgggt gctggcggcc acgaccttca ccgggcagtt cccgctggtc gcacggcgcc
    10861 agcggacgat cgaggcgtgg gagcagccag gccggccgcc tcggaccgat cctatccctg
    10921 ttgggacgcg ggtgttccac cagaaatttg gctacggcac ggtcacgtcg gctgaggacg
    10981 gcaggctcga cgtggccttc gataaggcgg gcgagaagcg ggtgctggac cggttcgtcg
    11041 aacaggtatg agaggaccgc ggcgccatgc gacgccgctg gagacggtgt cggtcgatgt
    11101 tcccgagttc gcgctggaag cctacgaggc ggcgctttcg accgcctgcg acactgtcgg
    11161 ctttttccac gaccaggaca ccggcgattg gcgggtcgaa ggggtaaagc cggtcggttc
    11221 aggcgaagcg gagctggcgg ccgctctgac acttgccgcc gcgctttcgg gagtagctgc
    11281 gtcgcttagt cgtgctggta ccccagccga gggctggctg gcacgcaccc gcgcagcatt
    11341 tcccgaacag tatgtcgggc gccgtttctc catgcgcggc acgcacctta ccggcgcgcg
    11401 ctgcgccggc cgcatcacgc ttattctcga tgctggcctg gcctttggct ccggagaaca
    11461 cggttcgacc cgcggctgtc tccgggccct ggagcaaatc gcttaccgcc ggccacgccg
    11521 aatcctcgat ttgggcacgg gatccggcat ccttgccatg gcggccgtac ggctactgca
    11581 tcgccccgtt ctggccaccg acatcgaacc gtggtcggtg cgcgtagggc gtgacaatgc
    11641 gcggcgaaac caactcgatc cgatgctgag gctccggctg gcagatgggt ggcggcatcg
    11701 cgtggtacga accgccgcgc cgtatgatct ggtcttcgcc aatatcctgg cgcgcccact
    11761 ctgtctgatg gcggttgggc tggcgcttca tctggcgccc ggcgggactg ccattctctc
    11821 gggtctgctg cagacgcagg cccgagcggt cgagacagcg caccgacgcc aagggttgcg
    11881 gctggagggg cgcatccagg aaggcccctg gacgacgctc atcctccgcc gggcaggaga
    11941 aactcagtaa gcgatgtagt cgcggccgcg cgcgtgatcc ttggcgaatg atggatcaaa
    12001 cacccggctg atgtccgacc ggctgaggcc gatatccgac agttcgcggt cgctcatcgt
    12061 ctgcatttcc tgcaacacaa ccatatgttg ccgccactcg gcgatcgcgc tcaccagcct
    12121 gccgaaccag gacttcgcct caccactggg cgaattgaca cgagcggtct gcggctcctc
    12181 ataacgaggt ccgatgtagg acaggttacc gagtgagaac tcgaattgat ctttggcgat
    12241 gggtgcgttc atgtcagttc ttccgatcac gcgaccagga ccggcgcagc gtcaccgcgg
    12301 cgaagcaccc ctggtcttat agtgcagtgc aacatagtgc ttgttgcggc tgtggataat
    12361 tgccgattcc ctccgacaga catgcgggag gcgcatgcct acctaatgag ttatgaaaat
    12421 gtagaagggt tggggaaatg cggagtaatg tgacaaccga gcctggcgaa cggcttaaaa
    12481 aattgcgcga cgagttgaag cgtcagcagc tggacggctt catcgtgccc cgcgccgacg
    12541 agcacctggg ggaatatgtg cctgccgcgg ccgagcgtct ggcatggctg accggcttca
    12601 ctggcagtgc cgggctggcg attgtgctgg ccgacaaggc agcggtgttc acggatggcc
    12661 gctatgtctt gcagctcgcg gagcagactg atccggcgct gtgggagcgt cgtcacgtca
    12721 ccgaggagcc gccgccgggg tggctcaacg ccaatgcgcc tgccggtgcg acgatcggtt
    12781 atgacccctg gctgttcgct gaagagggcg tggcgcgcta caccgacgcg ggcctgacaa
    12841 tgcggccggt cgaacgcaac ccggtcgatg tggtctggac cgatcggccg ccgcccccgc
    12901 tggtgcctgc cgaaccatat ccgctggaat atgctggccg ctccgccgcc gagaagcgcg
    12961 agcagatcgc agggctattg cgcgaggtca ggcaggatgc ggcggtgatc agcgatccgg
    13021 cctcgctggc ctggctgctg aatatccgag gcggggatgt gcagttcacg ccgttcgcgc
    13081 tcgggttcgc catcgcgcat gccgacggcg ggacagagct gttcatgcac ccggccaagc
    13141 tgcctgagcg aacccgtgcg tggcttggca atgcggtgtc ggtagccggc cgggaggcgc
    13201 tggcaccggc gctggcgaag ctggcgggaa agcgggttcg ggtggacggc gccggctcgc
    13261 cggtctggtt cgcgcagact ttgcgtgcgg cgggtgcctc ggtggtgtcc gctcctgatc
    13321 cgtgcctgct gccgaaggcg tgcaagaact ccgtggagca gcagggcacc cgtaacgccc
    13381 atgcccgcga tgccgtggcg gtgtgccgct tcctgcactt cctggccgaa accggtccgc
    13441 gcggcagcga gacggagatc tcggcggcgg caaggctgct ggcgttgcgc agcgaagtgg
    13501 agggctttcg cggcgaaagc ttcccggcga tctctggtgc cggcgagcac ggtgccatca
    13561 tccattatcg cgtgacggag gagagcaacc ggccgatcag gccgaacgag gtctatctca
    13621 ttgactcggg cgcgcaattt ccggacggca caaccgatat cacgcgcacc gtctggaccg
    13681 gcccgggcgc gccgccgcag ccgctgcgcg agcaggtgac gcgggtgatg caaggccata
    13741 tcgcgatcgc gaccctggtg ttcccccggg gcgtttgcgg tgcccacctg gacgcctttg
    13801 cccgccgtgc gctgtggcag gtcgggctcg actacgatca cggcacgggc catggcgtcg
    13861 gcagctatct ttcggtgcac gagggcccgg tcagcctgtc gcgtctggcg cggccggtgc
    13921 cgatcgaggc gggcatgatc ctgtcggatg aacctggcta ttaccttcct ggcgcgcatg
    13981 gcatcaggtt ggaaaatctg ctgctggtgc agccggcgga cctgtcaggc gcgaacaagc
    14041 cgttcctgcg gttcgagacg ctgaccttgg cgccattcga tcggcggctg atcgatccgg
    14101 cgatgctcac gaacgaagag ctggcgtggc tgaacgccta tcacgcacgt gtcctgtcgg
    14161 aggttggacc acaccttgat ggagcagccg gtgcgtggct gaagcgggcc tgcgcggtca
    14221 tcgaaaggtc caactgatgt ttcaccgctt tcgcatgggc aactcggctg cgaccattgt
    14281 gtctgacggg cctctgacct tgcccaccgc atcccggatc ttcgccggtc cggccaagga
    14341 tgcgctggat gccgcggtgt ccgcgaccgg ccagtccact gatgccgtgc gggtcgagca
    14401 gaactgcctg ctcctggaaa ccggggggaa gcgtgtactg ttcgacaacg ggcttggatc
    14461 agagaagctc tacgggcccg atagcggcaa gctgttggcc agccttgccg aggcaggtgt
    14521 tgatcctgcc tcgatcgacg ggatggtgct gacacacgcg cattctgatc actgctgggg
    14581 caccattggc gacgacggca cgccgaattt ccccaatgcc actctgtatc tttccaatgc
    14641 tgagatcgaa tactggcaga gcaatcctcc tggcgaacgt cgccagcgcg gcatcgccgg
    14701 tgtgcggaag catcttttgc cgctgcgcga tcgcatgcgc ttcattcgcg acggcgagga
    14761 gtttctccct ggcgtgcagg cgtggttcac gcctggccat acgccgggac acctggcgtt
    14821 ccttttttcg ggcggctggt gcctgaccgg cgacgtggct ttccacgatc cgatctccta
    14881 cgcgttcccc gaagccgaaa gcgcgtttga tgtcgatccg cagcttgccg tcgccacgcg
    14941 cggccgtttg ctcgatcggc tggtggatga gcgcatgcag gtgatcggct atcaccatcc
    15001 ctggccgggc cttggccgtg tggaacgtgt tgcgggcaca ttccgtttca tacgaaacga
    15061 atgacggctt cgactgttag agcctggtga gattcggtcg aatcacgcgt cgccccacgg
    15121 ctgggaccac gggttgtggg gtttgtgcgc cggtaacagc cctggagatt gaaaacacag
    15181 aacacgctga acacgcagat acatgccgaa aagggcaggg ttaggtgccg tccaggcccc
    15241 gtaacgcacc gctgcccttg gcgtgttctg tgtgctttgc ggttacttta ttcctttctg
    15301 tccctcaggc cgcgcccctg cgaaaccagg gcaactatgg accccagctg aattcgtcct
    15361 ggtttagcgt gggatcagcg tttgcagcgg caccgctggt gaggccagtg ccacctcatc
    15421 caccgggcgg cgcgccttga tcatcgcctg gccggcgcgg atctcgcggg ggttgttggc
    15481 ggcggtaacc ccgatcacgc agccatcctc ggcaaggtgc acggcggtga atgcctttgg
    15541 cggtgtattg acgcgcacga tggtccgcgc cgcgtctgcc ggcatgccgg caacctggag
    15601 gttcactccg tgctggtcgg tccagaacca cgggatgtcg tcatacggcg tggtgtcgcc
    15661 gcacatcgcc ttgccgacgg cgagtccgtg gttctgcgcg tggcgccatg attccagacg
    15721 aagtctgcga ttgaacagcg ggtggtggaa cgcggtcaca tcgcctgcgg cgtagatgtc
    15781 gggatcgctg gtgcggccga attcatcgac cacgatgccg ccttccatcg acaggccagc
    15841 cgcatcggct agcgccagat tgcgctgcat accaacgcct gcgatcacca gatcgccgat
    15901 gtagctgtca ccgtcacggc agacgacgcg catgccgcgg ggagtatcct cgatcgtatc
    15961 cacaatgacc ccgaagtgca gtgcaacgcc ggcatcgcgg tggagggtct caatgaatgt
    16021 cgcaccttcc ggcgacaccg cgcgccccat ggcgcgcggc agtgcctcaa gcaccgtaac
    16081 ctcacagccg cgagcgcggg ccgacgatgc tatctccagc ccgatcacgc cggctccgat
    16141 gcagatcacg ttgcgcgcgt tggacaggcg tgcgcggacc aggcgggcgt cctccaaggt
    16201 tcgtagatag agcgccttgt cgccgccatg gatgggcaaa tgacgggcac tgccacccac
    16261 cgtcagcagc agccggtcgt aatcgaatgt tcgaccatcg gccaggcgga tccgatgcgc
    16321 cgcagactcg atttcggcaa ccgcggtgcc gagcagcaat tcgatccgtt gctcggcgta
    16381 tcgctcctcg gcgtggaaat acgcgatcgg cggttccgtc gcttccgtga gtaccgcctt
    16441 tgacaggggc ggacgctcat acggccgcca gggttcgtcg ccgatcaaca ggatgcggcc
    16501 gtcgaagccg gcctgacgca taccgatcgc agcccacccg ccagcctggc cggccccaac
    16561 gataacgtgt gtcgtcatcc ggagcggcta acgcggtggg ggcttatcgg gcagccagac
    16621 atcggtccaa ccagtttcgc catcctggcc ggcaagcttg acctcggtcc ggcccggacc
    16681 ttcacgggag acgatctgaa cgcccaccgg ctgggcaatg gcgtggcagt ccgcggtctg
    16741 ctgctcgcca ccgctgacgc gcgcgatcat attggcagcg tgtcgggaca gatcgtcctc
    16801 tgttttgcag agcacggagc cggggtcgag ccgcggccag accgcggtag gcgcgcgggg
    16861 ggctggaggg ccagctttcg gctttggtgg caacaggctg ggagcatcca gaaccacatg
    16921 ttgcgccagc gcgggtgtgg cgaatgccag cagcaccgca gaaccaagta tgatctgcca
    16981 tttctcaggt ccaggcatgg cgcctccctc ccaaccaaag caagcgcgcg attctacgag
    17041 gcagcaggca ccagcggcaa ccatgacttg gccggttcgc cgggcactgg cattctggtg
    17101 ggaacggaac caggagaccg agatggcgga agcgggatgg gtgcgcgttg caggcgtgtc
    17161 ggacgtggcg gaaggccagg tacacgcggt gcgcgtcgga gatcgggaga tcgcggtcta
    17221 ccacctgcag ggtggcgact tccgcgcaac cgataatgtc tgcacgcacg aatacgcgct
    17281 gctgtctgat ggctggctgg aggatggctg catcgagtgc ccgctccacg cggcccggtt
    17341 cgatgtgaag accggcaagg cactgtgtgc tccggcagag acgaatctgc aggtgttcga
    17401 ggtaaaggtt gagggcgagg acctgttgct gaaggtgtag cgacgtgtcg cccatcgttg
    17461 actgcgatct gcatcccacg gtgccgtcac tggcggcgct catgccgtat ctccacgata
    17521 tgtggcgcga gaccgtggtg cggcgcgggc tcgatgagct gaacaccatc gcctatccgg
    17581 caaacagccc cctgacagcc cgcagcgact ggcgagacga acgtggtaag ccggccacca
    17641 cagcggagcg gctaggcaag gacgcgctcg atccatttgg cacacaggtc gctatctgta
    17701 actgcctcta cggcgtgcag gcgacgttca gcgaggacct gggcgccgcc atggctcgcg
    17761 cggtgaatga ctggattgcg cacgaatggc tggaccgcga cccgcggctg cgggcctcga
    17821 ttgtggtgcc gcagcagaat gcggaactgg cggtcgccga gatcgagcgc tgcgcgccgg
    17881 atcgccgctt cgtgcaggtg ctgttgctcg tctcgggcga actgccgctg gggcggcggc
    17941 agaactggcc gatctacgcc gcagctgagc ggctcaggct gcccgtcggc atccatgcgg
    18001 gcagcaccta tcggcatccg aacacgccgg tcggctggtc cagctatttg agcgaggagt
    18061 acatcaacca ggcgggtgcg tttcagaccc agctcaccag cctgatggcc gagggtgcgt
    18121 tcgccaagtt cccgaacctg accttcgtgc tgatggaatc aggcgtgacc tggctgcccg
    18181 gctatctttg gcggctcacg aagttctgga aggggctgcg gagcgaggtg ccgtgggtgg
    18241 cggacccgcc ggcctccatc gtgcgggacc gcgtccggct gacgttgcag ccgttcgacg
    18301 gtccgccgga aacggccacg atcacgcgct tcatggagca catgggctcc gacgaactgc
    18361 tgctgttctc caccgactat ccgcactggc agttcgacgg caccgacgcg atcccggcgg
    18421 ggcttgaccc ggcgctcgcg cagaagatca tggtcgacaa ccctcggaac acctatgctc
    18481 ggctcaggga aacaacccaa tgatggccgg gtcgggcccg gtcatgacca ccgggacaag
    18541 cccggtggca ggctcaagag gaccgggcta gcgcccggag tgaaatatga acgtggacgt
    18601 cctgaagcgc actggcctcc agacgccgaa ccagtccaag ctggcgatcg ccgactgcga
    18661 tattcatccc aggccgccag gagcaggcat cggcggggtg agcaaggcgc tgtaccccta
    18721 cctgtcaaag cgctggctgg agcatgtgca gacgatcggc gtgcgctatc gccagccctg
    18781 ggagcgtggt tccgcctacc cgaaagggca gccgcaggcc tgccgtcggg atgcctggcc
    18841 gaagggggac aatccgggca gcgaccttgc gttcatggcc gagcagcacc tggaccccaa
    18901 caacgttgcg ttcggcgtgc tcaatccgct gaccagcggc cagggcgcgc aggatccaga
    18961 gctgtcagcc gccctgaccc atgccaccaa cgagtggcag cttgccgaat ggacgtcgcg
    19021 cgatacgcgg ctgaaggcct cagtgatgac gccgttcgaa gacgccccca cagcggccaa
    19081 gatgatcgag gcgcgcgccg gcaatccgaa ctttgcccag gtgctgttgc tgacccgcac
    19141 cgccgaaccg ctcggccagc gccgctactg gccgatctac caggcggcgg tggaggccaa
    19201 tctgccagtc ggcatccatg cgtttggcta tggcggcagc gccctgacct cgggcggctg
    19261 gccgtcttac tacctggaag agatggtagg acacgcgcag tgccagcagg ccgtgctgac
    19321 gagcctgatc ttcgagggcg tgttcgaacg cttcccgacg ctgaagatcg tactgatcga
    19381 agccggcttc gcgtgggcag cgtcgcacgc ctggcggatg gatcggcagt ttgcgaagct
    19441 gcgcaaggag gtgccgcacg tgaagcggca gccgtccgag tacatgcgga gcaacgtctg
    19501 gttcaccacg cagcccgtgg aggaaccgga gccacgcgag catctcgccg atgcgatcga
    19561 atggatgggg tgggatcgcg tgctgttcgc gaccgattat ccgcactggg acttcgacga
    19621 tccggctcat gcgttgccga taaagatgac cgaggcgcag aagcgcggca tcttcctgga
    19681 gaacgccagg gcggtgtacg gcttcgcgtg aaccgcggcg acaagccatc gggcttcgat
    19741 ccgttctcat tgccagagag caatgcgac
//
LOCUS       DATGEM010000478         6699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1136900, whole genome shotgun sequence.
ACCESSION   DATGEM010000478 DATGEM010000000
VERSION     DATGEM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136900"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VKQ27_15020"
     CDS             <1..541
                     /locus_tag="VKQ27_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HLY90292.1"
                     /translation="SPYVALMRAIAHQQLHGRAAEAILARFECLYPDGDFPPPEMVLA
                     SPEDKLRACGFSGSKIAAMRAICAATLDGTVPTRRGSARFSDAELIERLTTIRGVGRW
                     TVEMLLIFTLGRPDVLPVDDFGVRDGYRHLYGLEVQPKPKALAEIGVAWAPYRSIAAW
                     YLWRASDEAKKAKVPTSRA"
     gene            complement(798..2027)
                     /locus_tag="VKQ27_15025"
     CDS             complement(798..2027)
                     /locus_tag="VKQ27_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HLY90293.1"
                     /translation="MQPKVDERRQWDRLMAMARIGAIAEDGVNRACLTELDRQARRLL
                     IGWARDIGASISVDAAANLWLRRDGTDPTATPVVTGSHMDTQPNGGRFDGIYGVIAGL
                     EALTALHDAGTTTRRPIEVVAWTNEEGGRFAPGCMGSMAWSGFRRIEEFAEITAPDGT
                     RFADALAVHLAAESDLPHRPLGSEPHAYVEAHIEQGPRLEAENKDIGIVTGIQGSRWF
                     TVTLTGDTAHAGTTPLGLRRDAVQDMVRAITALNALMHDPKDILRFTVGRIVVEPNTS
                     NSVANRASFTIDFRHPDKEVLLARGDAIAGVVQSAVRDAGVTVAETFHALPVEFAPTV
                     INAVERSAADQAYSAMHLPSGAFHDAQFVVPVCPTGMIFVPCRKGISHNPAEYSTPGQ
                     LAAGTNVLTQTLADLAA"
     gene            2293..3042
                     /locus_tag="VKQ27_15030"
     CDS             2293..3042
                     /locus_tag="VKQ27_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLY90294.1"
                     /translation="MAGHSQFKNIMHRKGAQDARRGRQFAKLIREITVAARQGLPDPA
                     SNPRLRAAMVAARQANMPKDTVDRAVKKAIGAGGGDDYAEVRYEGYGPAGVAVIVEAL
                     TDNRNRTASDVRSAFAKYGGALGETNSVSFLFNRLGVIQYPASAASADEMLEAAIEAG
                     ADNVESDVEAHEVTAAPEDFFAVRDALETRFGAPESARLDWRPTTSVTLDEDRAASVL
                     KLIDVLDDSDDVQNVYANFDIPDAVMQKLSA"
     gene            3127..3807
                     /locus_tag="VKQ27_15035"
     CDS             3127..3807
                     /locus_tag="VKQ27_15035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90295.1"
                     /translation="MIAAPAGYYENVRTDVEPLLPSRVCTALEIGCGAGATMAWLRQK
                     RQIEYAAAVEIVAEAASRAEAVFDDVVLGDINCARLEFKRRQFDLILALDVIEHLPDP
                     AAALASLHDHLEPNGTLIVSLPNVAHYHVSLPLFFLGKWEYRDQGLLDRTHMRFFTMS
                     SAVALIQDAGFQVRRVMLKRQFPNVFAAVGLTSQRWRWYSNRILGYLVPWPRHLFVHQ
                     ILIAAGKT"
     gene            complement(3965..4921)
                     /locus_tag="VKQ27_15040"
     CDS             complement(3965..4921)
                     /locus_tag="VKQ27_15040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY90296.1"
                     /translation="MIAESSDQPSPRVGIIVLNWNGWRDTVECLASLSSLTYDNFQVY
                     VVDNASTDGSETHLRARHPGLRILQSGSNLGWSGGNNVGIRAALAEGCEHILLLNNDA
                     TVRPDTLDPLVTAAGLPNAGALGSLILSADEPALAEFAGTIIDPSSHFPRQLSGPAND
                     VDPAPAASIAVKGCSMLLTGTALAQVGLLPDEYFLNYDETDWCYRAIAAGFINYLVPS
                     SVVFHKGAVAFQGTATPLYRYFIVRNRLLFAHRNLDGVGRRFAWRTTLWEIKSALLQP
                     QSLRHRALLLLAVTLGLVHYCLGRLGNCPSLVRWAHRAYLAA"
     gene            complement(4918..5529)
                     /locus_tag="VKQ27_15045"
     CDS             complement(4918..5529)
                     /locus_tag="VKQ27_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY90297.1"
                     /translation="MADELIFYTNPQSRGRIIRWMLEEIGQPYRTEILDYATTMKAPA
                     YLSINPMGKVPAISHGNTVVTECAAICSYLADAFPAANLAPAPADRGAYYRWLFFASG
                     PLEAAWTNQALGVTIPREKERMAGYGNLALVLDTLEAAVAKAPYIAGPSFTAADVYLG
                     SQLGFGLQFGMIEKRPAFVDYTGRLTARPAAIRAKEIDDAAAS"
     gene            5598..6092
                     /gene="ruvC"
                     /locus_tag="VKQ27_15050"
     CDS             5598..6092
                     /gene="ruvC"
                     /locus_tag="VKQ27_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLY90298.1"
                     /translation="MPRLLGIDPGLRFTGWGIIEAESNRLRHVADGVIATDGDLGVPE
                     RLKQLHDALAALLAEMRPDEAAVEETYVNRNGAATLKLGYARGVALLAPALVGVPVTE
                     YAAKSVKMAVVGTGGADKAQVQMMVRRLLPGATVRRADAADALAVAICHAHHRSSRLA
                     WGVA"
     gene            complement(6202..6408)
                     /locus_tag="VKQ27_15055"
     CDS             complement(6202..6408)
                     /locus_tag="VKQ27_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HLY90299.1"
                     /translation="MPDPVYKVVEVVGTSDQSVSKAIDNAIAKASETLRHLGWFEVMQ
                     IRGNVDSGKVNRYQVTLKVGFTLE"
BASE COUNT         1144 a         1791 c         2504 g         1260 t
ORIGIN      
        1 gagtccgtat gtagcgttga tgcgggcgat cgcgcatcaa cagctgcacg gtcgggccgc
       61 ggaggcgatc ctggcgcgct tcgagtgcct gtatcctgat ggcgatttcc cgccgcctga
      121 gatggtgctg gcgagcccgg aggacaagtt gcgggcatgc ggcttttctg ggtccaagat
      181 cgcggcgatg cgggcgatct gtgcggcgac actcgacggc acagtgccga cgcggcgcgg
      241 ttcggcgcgg ttttcggacg cggagctgat cgaacggctg acgacaattc gcggcgtggg
      301 tcggtggaca gtggagatgc tgctgatctt cacgctgggc cggccggacg tgttgccggt
      361 ggacgatttc ggtgtgcgcg atggctatcg gcatctgtat gggctggagg tgcagccgaa
      421 gccgaaagcg ctggctgaga tcggggtggc gtgggcgccg tatcgatcga tcgcggcgtg
      481 gtatctttgg cgcgcgtcgg atgaggcgaa gaaggcgaag gtgcccacgt cgcgggcata
      541 ggcgagatga tcggtccccg accgccgtct ttgcgatggt gttcggttgt gcaggcgggc
      601 aacgaggaaa atggaaccgc agaacacgca gatggcggcg gcgcctgaca aggccaggac
      661 agaggagtct ccccggtaac catctgcgtg ttctgcgtga tctgcggttc cattaaattg
      721 ctatcgaacc gaccgcgctg cgcggtaccg gggacatccg tgaaccgagc ggcgcaatcg
      781 tccagcctct ctccaagtca ggcggcgagg tcggcgagcg tctgcgtcaa tacatttgta
      841 ccggcagcga gctgtccggg cgtggagtat tcggcgggat tgtggctgat gcccttgcgg
      901 caagggacga agatcatgcc ggtggggcag acggggacga cgaactgcgc gtcgtggaag
      961 gcaccggagg gcaggtgcat ggcgctgtag gcctggtcgg ccgcggaacg ttcgacagca
     1021 ttgatcacgg tgggggcgaa ttcgacgggc agggcgtgga aggtttccgc caccgtaacg
     1081 ccggcgtcgc gcaccgccga ctgcaccacg ccggcaatgg catcaccgcg cgccagcagc
     1141 acctctttgt cggggtggcg gaagtcgatg gtgaagctgg cgcggttggc gaccgaattg
     1201 gaggtgttgg gctcgacgac gatacggccg acggtgaagc gcaggatgtc tttcgggtcg
     1261 tgcatcaggg cgttcagtgc ggtgatggcg cggaccatgt cctgcacggc atcgcggcgc
     1321 aggccgagag gcgtggttcc ggcatgcgcg gtatcgccgg tgagagtgac ggtgaaccag
     1381 cggctgccct ggatgccggt gacgatgccg atgtccttgt tctcggcttc gaggcggggg
     1441 ccttgttcga tgtgtgcctc gacataggca tgaggctcgc tgccaagcgg ccggtgcggc
     1501 agatcggatt ccgccgccag gtgcacggcg agcgcgtcgg cgaagcgggt gccgtctggg
     1561 gcggtgatct cggcgaattc ctcgatgcgg cgaaagccgc tccaggccat gctgcccatg
     1621 cagccggggg cgaagcggcc cccttcctcg ttggtccagg cgacgacttc gatgggacgg
     1681 cgggtggttg tgccggcgtc gtgcagtgcg gtgagggctt cgaggccggc tatcactccg
     1741 tatataccgt cgaagcggcc gccgttgggt tgagtgtcca tgtggctgcc ggtgacgacg
     1801 ggcgtggcgg tgggatcggt gccgtcgcgg cgaagccaga gattggcggc agcatcgacg
     1861 gatatgctgg cgccgatgtc tcgggcccag ccgatgagga ggcgacgggc ctgacggtcc
     1921 agttccgtga ggcaggcgcg gttgacgccg tcctcggcga tcgcgccgat acgtgccatg
     1981 gccatgaggc gatcccactg gcggcgttcg tcgacctttg gctgcatcgg cttggtcctg
     2041 tactgcggtg gcgcaggtta tgtcaggtca gagcgcgggc ggcgagatca gcgccgtgcc
     2101 gcgccaggct gatcgtggcg tagacgtgac ggtccgctgt cagggcgaat ctcggcagca
     2161 tggacaactt gagggtatgc atgggattgc gtattcggcg gtctggcggc cggggcaatg
     2221 ccgggtggcc cggcctcggc ggaactgtcc ggttggggta gagttggagc gaaggcacgt
     2281 atcgatagga ccatggctgg acattcacag ttcaagaaca tcatgcaccg caagggcgcg
     2341 caggacgccc gccggggacg gcagttcgcc aagctgatcc gggaaatcac tgtggcggcg
     2401 cggcaggggt tgcctgatcc cgcgtcgaac ccccggctgc gcgcggcgat ggtggcggcg
     2461 cggcaggcca atatgccgaa ggacacggtg gaccgggcgg tcaagaaagc catcggcgcc
     2521 ggtggtgggg atgattacgc ggaggtccgg tatgagggat atgggccggc gggcgtggcg
     2581 gtcatcgtcg aggcgctgac cgacaaccgg aaccgcaccg cgtcagatgt acgctcggcg
     2641 ttcgccaagt atggcggggc gctcggggag acgaactcgg tcagcttcct gttcaaccgg
     2701 ctgggtgtga tccagtatcc ggcctcggcg gcgagcgctg atgaaatgct cgaggcggcg
     2761 atcgaggcgg gtgcggataa tgtcgagagc gacgttgagg cgcacgaggt gaccgcggca
     2821 ccggaagatt tcttcgctgt gcgggatgcg ctggagacgc ggttcggtgc gccagagagt
     2881 gctcggctgg actggcggcc cacgacgagt gtgacgctgg atgaagaccg ggccgcatcg
     2941 gtgctgaagc tgatcgacgt gctggatgac agcgacgacg tgcagaacgt ctatgccaac
     3001 ttcgatatcc cggatgcggt gatgcagaag ctgtccgcgt aggcgggaag tcagccgact
     3061 tttcacgtta atcatgtcct agaacgacac ttgcgtgctg ccgcaaagcg cgcaagctgc
     3121 ggtgggatga ttgctgcgcc cgcaggctat tacgagaatg tccgtaccga cgtggagccc
     3181 ttgcttccgt cgcgggtgtg cacggcgctt gagatcggtt gcggtgcagg cgcgacaatg
     3241 gcctggctgc gacagaagcg gcagattgag tatgcggctg ctgtcgagat tgtggcggag
     3301 gccgcaagca gggccgaagc tgtgtttgat gatgtggtac tgggcgatat caattgtgcc
     3361 cggttggagt tcaagaggcg ccaattcgat ctgatactgg ctctcgacgt gattgagcat
     3421 ttgcctgatc cggcggcagc tttggcatca ctccacgatc acctcgaacc gaatggcacc
     3481 ttgattgtat cgctgcccaa tgttgcccat tatcacgtct cactcccgtt gttcttccta
     3541 ggcaagtggg agtaccgcga tcaggggctg ctggaccgca ctcacatgcg gttcttcacc
     3601 atgagcagcg cagtggctct tatccaggat gccgggttcc aggtgcggcg tgtgatgttg
     3661 aagcggcagt tcccgaatgt ctttgctgct gtcggactta cgtcgcaaag gtggcgttgg
     3721 tacagcaaca ggatactcgg gtatctcgtg ccgtggccgc ggcacctttt tgttcatcag
     3781 atccttatcg cggcgggcaa gacgtaaatc gggccgttgc gtttcatcgc ctcctgtaat
     3841 tgcgggccct gcgcgggcac attggcgcgt cgggaggctg atggccggct caaggccggc
     3901 cataacggaa ggggtgcagt caacatggct tcggctgcat cggtgccacc aatgcttgag
     3961 gcggttacgc tgccaggtag gcccggtgtg cccagcgtac cagtgaagga caattgccca
     4021 ggcggccaag gcagtagtgg accaggccca atgtgactgc caggagcagc aacgcacgat
     4081 ggcggaggct ctgtggctgc aggagtgcgc ttttgatttc ccacagcgtg gtgcgccagg
     4141 cgaagcggcg accgacacca tcgaggttgc gatgcgcgaa caggagccga ttgcggacga
     4201 tgaagtagcg atagagtggt gttgcagtgc cttggaacgc aacggctcct ttgtgaaaga
     4261 caactgaaga gggcaccagg taattgatga agcctgcggc aatggcgcga taacaccagt
     4321 cggtttcgtc gtaattcagg aagtattcgt ctggcagcaa gccaacttgc gccaaggccg
     4381 tcccggtcag cagcatcgag caacctttga ccgcgatcga tgcggcgggg gctggatcga
     4441 cgtcgttcgc gggcccgctg agttggcgcg ggaaatggct tgagggatca atgatggttc
     4501 cggcgaactc ggcgagggcg ggttcatctg ccgagaggat caacgaacca agggcgcctg
     4561 cgttgggcag tcctgccgcc gtcaccagcg gatcgagcgt gtcgggccgc acggtggcat
     4621 cgttgttgag cagaaggatg tgctcgcaac cctcggccag cgcggcgcgt atgccgacat
     4681 tgttgccgcc actccagccg agatttgatc cggattggag gatgcgaagc ccggggtgcc
     4741 gggccctgag atgggtttcc gagccgtctg tcgaagcatt gtcgacaacg tagacctgaa
     4801 agttgtcgta ggtgagagag gaaaggctgg cgaggcactc taccgtgtcc cgccagccgt
     4861 tccagttcag gacgatgatg ccgaccctcg gactgggttg atctgatgat tccgcgatca
     4921 agaggcggct gcgtcgtcga tttccttcgc ccggatcgcg gccgggcggg cggtgaggcg
     4981 gccggtatag tcgacgaagg cggggcgctt ctcgatcatg ccgaattgca ggccgaagcc
     5041 gagctgcgag ccgaggtaaa cgtcggcggc ggtgaaggag ggaccggcga tgtagggggc
     5101 tttggcgacg gcggcttcaa gggtgtccag cacgagggct aagttgccgt agccggccat
     5161 gcgttctttc tcacgcggaa tggtgacgcc gagggcctgg ttggtccagg cggcctcaag
     5221 cgggcccgag gcgaagaaga gccagcgata gtaggcgcct cggtcggccg gggccggagc
     5281 gaggttggcg gctgggaagg catcggccag gtaagagcag atggcggcgc actcggtgac
     5341 cacggtgttg ccgtggctga tggctggcac tttgcccatc ggattgatgg acaggtacgc
     5401 gggcgccttc atggtggtgg cgtagtcgag aatctcggtg cggtagggct ggccgatttc
     5461 ctccagcatc cagcggatga tgcggcctcg ggactggggg ttggtataga agatcagttc
     5521 gtcagccata gggatctcct ttcggtgggg ttgaggggta tatcgacaca agacaggaac
     5581 aggggggcga ttcgaccatg ccgaggctgc tggggatcga tccggggctg cgcttcaccg
     5641 gctgggggat aatcgaagcc gaaagcaacc ggttgcggca tgttgccgat ggagtgatcg
     5701 cgaccgacgg tgacctcggg gtgcctgagc ggctgaagca gctgcacgac gcgctggcgg
     5761 cgttgctggc ggagatgcgg ccggatgagg cggcagtgga ggagacgtat gtcaaccgga
     5821 acggcgcggc cacgctgaag ctggggtatg cgcgcggggt ggcgctgctg gcgccagccc
     5881 tggttggggt gccggtgacg gaatacgccg cgaagtcggt gaagatggcc gtggtgggga
     5941 ccggcggggc cgacaaggcg caggtgcaga tgatggtgcg ccggctgctg ccgggagcga
     6001 cggtccgccg ggcggatgcg gcggacgcgc tggcggtggc catctgccat gcgcatcacc
     6061 ggtcgagccg gttggcgtgg ggagtggcgt agtctcgcgg ggggcgccag tgggggcggc
     6121 gcagcggtgg agatgggcgg caccgttcga cttcctgtgt tcctgatatg ttctagtttg
     6181 gtgcgcgggc gctggcaacc gctattcgag ggtgaagccg accttcaggg tgacctgata
     6241 gcggttgacc ttgccgctat cgacgttgcc ccggatctgc atgacctcga accatccgag
     6301 atgccgcaat gtctccgacg ccttggctat ggcgttgtcg atggctttgg agacgctttg
     6361 gtcggaggtg ccgacaacct cgacgacctt gtagaccgga tcgggcattt cgctctccct
     6421 ggatggttgt tgttttgctg tggggcgggg cttccttttc ggctcgaacg tgtcctggcc
     6481 acgcgatcgc tcgatgctgc ggcggcactg ggcgcgaggc aagcctgcta agagggcgtg
     6541 cttgcccgct gcgggatcaa ccagtacagc tcgcaaaact tgcctgaacg aaacgggagg
     6601 ataggcatga tgagcagatc cgtactcatg attttcggga tgatggccgt ggcgagctgc
     6661 gtggcgttga tgccggcgag ggcaagcagt agcgatgag
//
LOCUS       DATGEM010000479         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_375178, whole genome shotgun sequence.
ACCESSION   DATGEM010000479 DATGEM010000000
VERSION     DATGEM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375178"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="purN"
                     /locus_tag="VKQ27_15060"
     CDS             complement(<1..517)
                     /gene="purN"
                     /locus_tag="VKQ27_15060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HLY90300.1"
                     /translation="MRLRVGILISGRGSNMASLIAAAREKDFPAEIVLVVSNRPEAAG
                     LELARQAGVKAVAIDHKAFRGDRAAHEAAVDEVLREAGVEIVCLAGYMRLLTPRLIEP
                     WQGRMLNIHPSLLPAFPGLHTHERALAAGVAEHGCTVHVVTQTMDEGPILAQASVPVL
                     AHDTAETLAVRV"
     gene            826..1608
                     /locus_tag="VKQ27_15065"
     CDS             826..1608
                     /locus_tag="VKQ27_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY90301.1"
                     /translation="MFAESAPGFARTPTPDNAGGPASAPTVLVIDDDTAMQHMLRGYL
                     EPHNVHVLSALRREDAIRQLTTRKPDIIVLDLRLGSDDGLDLLRDIRARSDVPVIITT
                     GHRRDEIDRVVGLELGADDYITKPFGPRELLARIRAVLRRQELGRTAWQRAVEIGRFR
                     FGGWELDRRNRRLTDPGGALVTLTKGEYALLVAFLDAPQRPLSREQLLQATRVHEDVF
                     DRSIDVQILRLRRKLEGNPGAPRIIQTERGVGYVFTLPVEHL"
     gene            1725..2213
                     /locus_tag="VKQ27_15070"
     CDS             1725..2213
                     /locus_tag="VKQ27_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90302.1"
                     /translation="MRNYLTTSADRTGAPPPNSVVLHVWGPPLKPRPFAAAVSWLMTS
                     FIDGMAAYGEMICPCLVDLPDPHSDQKQPGAAAPWPLYRQSQPDETSPPRPAPPPARS
                     GIAVRLGRWLAWLRPRRDHAPRAFAHERLDERTMRDIGLPPYHPDDLARYLERYADHG
                     GW"
     gene            2207..3202
                     /locus_tag="VKQ27_15075"
     CDS             2207..3202
                     /locus_tag="VKQ27_15075"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HLY90303.1"
                     /translation="MVAAPGGGAAPTIRRTAWWLAWLAGLSLVLAHDLAAEPPAAQAD
                     APPPVADQEPITPIPDPPAADPGRLALGERLFNDRRLSHDGTLACSSCHDIHTNGADG
                     RLHHIAGAIGKPPLATLTVFNAALSFRLNWQGNVRTLEGQMEASIGNPANMASSVTEV
                     LSKLDADTTIVRQFRQAYGREPDKTSLLDAIAVYERSLLTPDSRFDQWLKGDRTALSA
                     QEQHGYVLFQSLGCVSCHQGVNVGGNLYERHGIFHPLASPWPEVLRVPSLRNVAALAP
                     YFHDGSAPTLEDAVREMAVAQLDQALTDDQVAAIIGFLKTLTGKYRGVTVTAPEQ"
     gene            3199..>4483
                     /locus_tag="VKQ27_15080"
     CDS             3199..>4483
                     /locus_tag="VKQ27_15080"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAHL domain-containing protein"
                     /protein_id="HLY90304.1"
                     /translation="MRTLSATAVTLLLIALLTWLSIRAVNPDAERFDLALGELDRFAT
                     AQAMLHSDTLSARAGLLRNYDPPVRDEDALDASLKRVQVILADDTGTELALSLLAASV
                     RRQEDLIEQFKTDNALLRNSLAYFARFSAFLSAPDQANPLASAASALGGAMLRLTLNT
                     SAAAVRDVQNRSDDLARQLSPPVSNAAAVALLAHARLLTGLLPATDTLLRELLSVQGK
                     RSQDALRSILLAQQLDSRNTARKFRLVLYLTSLLLVALLVFAGVQLRARARTLRRKAA
                     FERVLARVSMDFASAAEADIEAIVAHALGAMAHWLGADRAWFLGSGRLRQMITWCRDG
                     LSFPPGWPDRAPSLLARHHYPRTDTVVHVPTIERLPLDATREALAAAGLRGWTCATAT
                     LADGTEVLMGFDAIAHASRIRPGEPGLLRVALDTIV"
BASE COUNT          724 a         1681 c         1348 g          730 t
ORIGIN      
        1 gcacccgcac cgccagcgtc tccgccgtat cgtgcgccag caccggcacc gacgcctgcg
       61 ccagtatcgg cccttcgtcc atcgtctgcg tcaccacgtg cacggtgcag ccatgctccg
      121 cgacgcccgc cgcgagagcc cgctcatgcg tgtgcagccc gggaaacgcc ggcagcaggc
      181 ttggatggat gttgagcatc cttccctgcc atggctcgat cagccgcggc gtcagcaggc
      241 gcatataccc ggcgaggcac acgatctcca cacccgcctc ccgcaacact tcatcgaccg
      301 ccgcctcgtg cgccgcccga tcgcccctga atgccttgtg gtcgattgcg accgccttca
      361 ctcccgcctg ccgcgccaac tcaagccccg ccgcctccgg ccggttcgac accacgagca
      421 cgatctccgc tggaaaatcc ttctcacgcg ccgccgcgat aagggaagcc atgttcgacc
      481 cgcgcccgct gatcaggatt ccaaccctca gtcgcatgcg ccgctctcct tcatccatcc
      541 atgatcacca agcgcgcagc cgggtcgcgt ccggctgccg cggcaaccag cgcatgtgtg
      601 acattcaccg cagtcatttt ccggcgccag gcatcaccaa tccaaccctt gcgatcgctg
      661 cgccaccgcc caagttctgg cggcgattct ccgtggtggc ggctggcatc agaccagccc
      721 gtggggccgc cacttctggt caaccccacc acgctttgta gcacacgcgt cgcgaaccag
      781 cgcgcaccgc cggggggata cgtcataacc ttggagttat gacctatgtt cgcggaatcc
      841 gcgccgggat ttgcccgcac ccccacgccc gacaatgccg gcggccccgc ctcggcccca
      901 accgtgctcg tcatcgacga cgatacagcg atgcagcaca tgctgcgagg ctatctcgag
      961 ccgcacaatg tgcatgtcct ctccgccctg cgcagggagg atgcgatccg tcagctgacc
     1021 acgcgcaagc cggatatcat cgtcctcgac ctccggctcg gcagcgacga tggcctggac
     1081 ctgctgcggg acatccgcgc ccgctccgac gtgccggtga tcatcacgac cggccatcgg
     1141 cgcgacgaga tcgaccgcgt ggtcgggctc gagctcggcg cagatgatta catcaccaaa
     1201 ccatttgggc cgcgcgagct tctggcgcgc atccgggccg tgctgcgccg ccaggagctg
     1261 ggccgcaccg cctggcagcg cgctgtcgaa atcgggcgct tcaggttcgg cggctgggag
     1321 ctcgaccggc gcaaccgccg cctgaccgac cccggtggcg ctctcgttac gctcaccaag
     1381 ggcgaatacg ccctcctggt cgcgttcctt gacgcgccgc agcggccgct cagccgcgag
     1441 cagctgctcc aggcgacacg ggtccacgag gatgtattcg atcgcagcat cgacgtgcag
     1501 atcctgcggc tgcgccgcaa gctcgaaggc aatcccggcg cgccgcgcat catccagacc
     1561 gaacgcggcg tcggctatgt cttcaccctg cccgtcgagc atctttgacg cggccgccgg
     1621 atgcgatcct cagcgctaca gccgtaacct tgatcgcgca gaccggaatg cttccgcaac
     1681 cgggcgccgt cagcttgacc ctcggcattc gaagggagag cctgatgcgc aactacctca
     1741 cgacatccgc cgacaggacc ggcgcgccac ccccgaacag cgtggtcctg cacgtttggg
     1801 gtccgccact gaagccacgg ccgttcgcgg ccgccgtgtc ctggctgatg acctccttca
     1861 tcgatggaat ggcggcatac ggcgagatga tctgcccatg tcttgtcgat ctgccggatc
     1921 cgcactcgga tcaaaagcag ccgggcgccg ccgctccgtg gccattgtac cgccagtcac
     1981 agcccgacga aacgtccccg ccgcggcccg ccccacctcc tgcacgcagt ggaattgccg
     2041 ttcgcctggg gcgatggctg gcatggctcc gccctcggcg cgaccatgcg ccgcgagcat
     2101 tcgcgcacga gcggctggac gagaggacga tgcgtgacat cggcctgcct ccctaccacc
     2161 ccgatgacct cgcgcgttat ctcgagcgat acgcggacca cggcggatgg tagcagcgcc
     2221 cggcggcggg gccgcgccca ccatccggcg aaccgcctgg tggctggcgt ggttagccgg
     2281 gttgtcgctc gtccttgccc atgacctcgc agcggagcca ccggccgcac aggcggacgc
     2341 cccgccaccg gtcgcggacc aggagccgat cacgccaata ccggatccgc cagcggccga
     2401 tccgggcagg ctcgcgctcg gcgagcgcct gttcaacgat cggcgcctgt cgcacgacgg
     2461 cacgcttgcc tgctcgtcgt gccacgacat ccacaccaac ggcgccgacg gccgccttca
     2521 tcatatcgcc ggcgccatcg ggaagccgcc gctcgccacg ctgacagtgt tcaatgccgc
     2581 gctgagcttt cgccttaact ggcagggcaa tgtgcgcacc ctggaagggc agatggaggc
     2641 ctcgatcggg aacccagcca atatggcgag cagcgtcacc gaagtgctca gcaaactgga
     2701 tgccgacacc accatcgtgc ggcagttccg gcaggcctat ggacgggagc ccgacaagac
     2761 cagcctgctc gatgcgatcg ccgtctacga acgttcgctc ctgaccccgg acagccgctt
     2821 tgatcagtgg ctgaagggcg accgcactgc gctgtcggcc caggaacagc acggctacgt
     2881 gctgttccag tcactcggct gcgtttcctg ccaccagggc gtcaacgtcg gcggcaacct
     2941 ctacgaacgg cacgggatct tccatccgct cgcctcgccg tggccagagg ttctgcgcgt
     3001 gccaagcctc cggaacgtcg ccgcgctcgc cccctatttc cacgacggca gcgccccgac
     3061 gctcgaggat gccgtacgcg aaatggcggt ggcgcaactc gaccaggccc tgaccgatga
     3121 ccaggtcgcg gccatcatcg gattcctcaa gacactgacc ggaaaatatc gtggcgtcac
     3181 ggtcaccgcg ccggagcaat gaggacactt tccgccaccg cagtcacgct gctgctgatc
     3241 gctctgctca cctggctgtc gatccgtgcg gtcaaccccg acgccgagcg cttcgatctc
     3301 gcgctcggcg agctcgaccg gtttgcgacc gcgcaggcga tgctgcacag cgatacgctc
     3361 agcgcgcggg ccggattgct gcgcaactat gacccgccgg tgcgcgacga ggacgcgctc
     3421 gatgcttcgc tcaaacgcgt ccaggtcatc ctcgcggacg acacgggaac agagctcgcg
     3481 ctgagcctgc tcgctgcttc cgtgcgccgg caggaagacc tgatcgagca attcaagacc
     3541 gacaacgcac tgttgcgaaa ctcgctggca tatttcgccc ggttcagcgc ctttctgagt
     3601 gcacctgatc aggccaatcc attggcgtcg gcggccagcg ccctcggcgg cgcgatgctt
     3661 cgcctcacgc tcaacacctc cgcggcggcc gtgcgcgatg tgcagaaccg atcggacgat
     3721 cttgctcgcc agctgtcgcc gccagtcagt aacgccgcag ctgtcgcact gctcgcacat
     3781 gcacgtctgc tcaccggcct gctgccggct accgacaccc tcttgcggga actcctgtca
     3841 gtccagggca agcgcagtca ggacgccctg cgttcgatcc tgctggccca gcagctcgac
     3901 tcgcgcaaca cagcgcgcaa gttccggttg gtgctctacc tcacgtcgct gctgctcgtg
     3961 gctctgctgg tgttcgccgg cgtccagctc cgcgcacgcg cccgcacgct gcgccgcaag
     4021 gccgcgttcg agcgtgtgct cgcccgcgtc tcgatggact tcgccagcgc agccgaagcg
     4081 gacatcgagg caatcgttgc tcacgcattg ggcgccatgg cgcactggct cggtgccgac
     4141 cgcgcctggt tcctcggctc gggcaggctt cgccagatga tcacctggtg ccgtgacggc
     4201 ctgtccttcc cgcctggctg gccggaccgc gccccatcgc tgttggcgcg ccatcactac
     4261 ccgcgcaccg acaccgtcgt gcatgtcccc accatcgagc gcctgccgct ggacgccacc
     4321 cgcgaggcac tcgctgcggc ggggctgcgg ggctggacgt gcgccaccgc aactctcgcc
     4381 gacggcaccg aggtgctgat gggattcgat gccatcgccc atgccagccg cattcgcccc
     4441 ggcgagcccg gtctgctgcg cgttgcactg gataccatcg tca
//
LOCUS       DATGEM010000480         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_69410, whole genome shotgun sequence.
ACCESSION   DATGEM010000480 DATGEM010000000
VERSION     DATGEM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69410"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VKQ27_15085"
     CDS             complement(<1..913)
                     /locus_tag="VKQ27_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90305.1"
                     /translation="MDGALARTVSRGASVVLSGVEKHFDSVGAVRGVSLDIRSGEFLT
                     LLGPSGSGKTTTLMMIAGFEMPNAGDIAIDGRSVVALPPHKRNIGMVFQNYALFPHLT
                     VAENIGFPLRQRGVDRATRARLVAGSLELVRLPGYEKRYPRQLSGGQQQRVALARAIV
                     FQPRLLLMDEPLGALDKQLRESLQLEMRRLHADLGITFIHVTHDQEEALTMSDRVAVM
                     NDGLIAQVGTPEDLYNRPCDQFVASFIGESNFLPGAVLGREGELLVVDCGAAIVRAVA
                     GDGRTHGGNVTVTTRPERMHFADRVAES"
     gene            complement(913..2031)
                     /locus_tag="VKQ27_15090"
     CDS             complement(913..2031)
                     /locus_tag="VKQ27_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90306.1"
                     /translation="MTRYDPDRRRFMALAAWATAGMSAIGARHAVAQTAPANMPEITS
                     IPEKLKGTGEVRIAAYGGTAQDAERIAYFKPFEKLSGIKTSDFAGADINKVKAMVETG
                     NVQWDVVQLGRSTVKNLMKKGDYFEKIDYELVDVTNIDPLYRYDYALDMLVWSEVMAY
                     RTDAFGGKIPVGWTDFWDTKKFPGDRSLGGAGPSTPELEFALMAAGVPPDKVYPIDVD
                     KAFASYDKIKASVVKWWETGAIPPQLLTDKEVVLTSVWNGRMAALQTAGVPAAISWDQ
                     GLLKRDCWAVPKGSPNKENAMKFIAFSTLAISQARLSMLIPYGSVNNKSAEYMTEQQL
                     AILPSAPAIKAKLVPYNYDWWVDNRDAVIAHWNKWILA"
     gene            complement(2044..3360)
                     /locus_tag="VKQ27_15095"
     CDS             complement(2044..3360)
                     /locus_tag="VKQ27_15095"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439016.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HLY90307.1"
                     /translation="MAAKPFHLGWFLQGSSVQAWGESWTGHIGTTWMVPEMFVDMARS
                     LERACFDYVLLEDSSYVGESWGGSTELYLKHAIAVPRQDPSVVASIMTQATSRIGIVP
                     TFGTYAYPPYLLARLIATLDQVSSGRIGWNAVTGSSDFAAMNFGMQGMPEHDLRYEMA
                     DEYMEVCRQLWGSWEPGAIIADRTNGILVDHTKVHAVNFEGKYFKTRGPLNSGPCVQG
                     QPVIAQAGGSPRGREFAAQYADTIVASTKGAAAMRAYRDDVRARMASCGRDPDSCKVL
                     FLVAPIIGETEEEAQEKKRLRQARAEAQVPQRLAHLGKVTNIDFGKFDIDKPLPEGVT
                     TNGHQQTLDEFRRKYAGKTLREAMLEHNTTALSVELVGTPDSLAARMAEVMEEVGGDG
                     FLFSLPNVSRRTLAEVEDGLVPALQDRGLVRAAYTHRMFRDNLLEF"
     gene            complement(3472..3945)
                     /locus_tag="VKQ27_15100"
     CDS             complement(3472..3945)
                     /locus_tag="VKQ27_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY90308.1"
                     /translation="MDAVVANETYRVLGQRPGFLVRRLHQIHLALFAEECKVFGVTPV
                     QYSILTVAAAQPGLDQAALANQVGVDRTTMADVLARLERRGLVRRTRVERDARRKLVH
                     ATVAGQQMLARMDGPAQQAHERTVAALPPKQRAAFIELLHRLVEASGDLGRAQRV"
     gene            complement(3945..>4395)
                     /locus_tag="VKQ27_15105"
     CDS             complement(3945..>4395)
                     /locus_tag="VKQ27_15105"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLY90309.1"
                     /translation="VPGARLLSVEGDQLRGEVRASLGPIETLFSGEGQVAFDAAEHRA
                     EISGDGRDGRSGTRLSGRAVVRLHEVGATTTRAEATIEYTLRGPLAQFARGAVVQDFA
                     ASIATTFAANLEARLTGRVVSAPARLPIGTLMLRAIWRRVRAILGLD"
BASE COUNT          838 a         1574 c         1164 g          819 t
ORIGIN      
        1 cgctttcagc cacacggtct gcgaaatgca tgcgctccgg ccgcgtcgtt acggtaacat
       61 taccgccatg cgtccgcccg tcccccgcta ctgcacggac gattgcggct ccacagtcca
      121 ccaccagcag ttctccctct cgccccaaca ctgccccagg caaaaaattc gactcgccaa
      181 tgaacgacgc gacaaactgg tcgcatggac ggttgtatag atcttcggga gttccgactt
      241 gcgcgatcag tccgtcgttc attaccgcga cgcggtccga catcgtcagc gcttcttcct
      301 ggtcgtgcgt cacgtggatg aacgtgattc ccagatcggc gtgcagccgt cgcatctcca
      361 gttgcaggct ttcgcgcaac tgcttgtcca acgccccgag cggttcatcc atcagcaaca
      421 gccgcggctg gaacacgatc gcccgcgcca acgcgaccct ctgctgctgc ccacctgaaa
      481 gctgccgcgg ataccgcttt tcatagccag gcagccggac cagctccagg cttcccgcaa
      541 ccagccgtgc ccgcgtcgcg cgatccacgc cccgctgcct cagcggaaag ccgatattct
      601 ctgccacggt caaatgtgga aacagcgcgt agttctgaaa caccatcccg atattgcgct
      661 tgtgcggtgg caacgcgaca acggagcggc catcgatcgc gatatcaccc gcattcggca
      721 tctcgaaccc ggcgatcatc atcagcgtgg tcgtcttgcc ggacccgctc ggtcccagca
      781 gcgtcaggaa ctcgcccgag cggatatcca gtgacacgcc ccgcaccgcg ccgacactat
      841 caaagtgctt ctccacccca ctgagcacaa ccgaagcacc tcgcgacaca gtccgcgcca
      901 gcgccccatc catcaggcga ggatccactt gttccagtgc gcgatcacag catcgcggtt
      961 atccacccac cagtcatagt tgtacggcac cagcttcgcc ttgatcgcag gcgcgctggg
     1021 caggatggca agctgctgct cggtcatgta ctcggcggac ttgttgttga cggaaccata
     1081 agggatcagc attgacagcc gggcctgcga aatcgccagc gtgctgaatg cgatgaactt
     1141 catcgcgttt tccttgttcg gcgagccctt cggcaccgcc cagcaatccc gcttcagcag
     1201 cccctgatcc caactgatcg ccgccggcac ccccgcagtc tgcagcgctg ccattcgccc
     1261 gttccacacc gaggtcagca ccacttcctt gtccgtcagc aattgcggcg gaatcgcacc
     1321 agtttcccac cacttcacca cgctcgcctt gatcttgtcg tagctggcaa aggccttatc
     1381 gacatcgatc ggatacacct tgtccggcgg cacaccggcc gccatgagtg cgaattccag
     1441 ttccggcgta ctcggcccgg cgccgccgag cgaacgatca cccgggaact tcttggtatc
     1501 ccagaagtcc gtccaaccaa ccgggatctt cccgccgaac gcgtcggtgc gatacgccat
     1561 cacctcggac cacaccagca tatccagcgc atagtcatag cgatacaacg gatcgatgtt
     1621 ggtgacgtcc accagctcgt agtcgatctt ctcgaagtag tcgcccttct tcatcaggtt
     1681 cttgacggtg gatcggccga gctgcaccac gtcccactgc acgttgcccg tttccaccat
     1741 cgccttcacc ttattgatgt cagcgccggc aaaatcagaa gtcttgatgc cgctcagttt
     1801 ctcgaacggc ttgaaatatg ctatgcgttc ggcgtcctgc gcagtaccgc cataggccgc
     1861 aatgcgcacc tcgcctgtgc ccttcagctt ctccgggatc gaggtgattt ccggcatgtt
     1921 cgctggtgct gtttgcgcga cggcatgcct ggcgccaatg gccgacatgc cggcggtcgc
     1981 ccacgcggcc agggccatga agcgccgacg atctggatcg tatctggtca taccggcctc
     2041 cgttcagaat tcgagcaggt tgtcgcgaaa catgcggtgt gtatacgcgg cacgcaccag
     2101 gccacgatcc tgcaaggcag gcaccagccc atcctcgacc tcggccagag tgcgccggct
     2161 gacattcggc agcgagaaca aaaacccgtc gcctccaacc tcttccatca cttcggccat
     2221 gcgcgcggca agcgaatctg gcgtgccaac aagttcgacg gaaagcgccg ttgtgttgtg
     2281 ctccagcata gcctcacgca gcgtctttcc ggcgtatttc cgtcggaact cgtccagtgt
     2341 ctgctggtga ccattggtgg tgacgccttc cggcaacggc ttgtcgatat cgaatttgcc
     2401 aaaatcgata ttcgtcacct tgccaagatg cgcgagacgc tgcggcacct gcgcctcagc
     2461 ccgcgcctga cgcaggcgct ttttctcctg cgcttcttcc tcggtctcgc cgatgatcgg
     2521 tgcaaccagg aacagcacct tgcacgagtc cggatcgcgc ccgcacgacg ccatgcgcgc
     2581 acgtacatca tcacgatacg ccctcatcgc ggcggcgccc tttgtcgaag cgacaatcgt
     2641 atcggcatat tgcgcggcga actcccgccc ccgcggcgag cctcccgcct gcgcaatcac
     2701 tggctgcccc tgcacgcacg gccccgaatt cagcggccca cgggtcttga agtatttgcc
     2761 ctcaaaattg accgcatgca ccttggtgtg atccaccaga atgccgttcg tccgatcggc
     2821 gatgatggcc ccaggctccc acgaacccca aagctggcgg cagacctcca tgtattcgtc
     2881 agccatctca tagcgcagat cgtgctccgg catcccctgc atgccgaagt tcatagcggc
     2941 aaaatccgaa ctgccggtca ccgcattcca gccgatgcgc cctgacgaca cctgatccag
     3001 cgtcgcgatc aggcgtgcca gcaaatacgg tggataagcg taagtgccga aggtcggcac
     3061 aatgccgata cgcgacgtcg cctgcgtcat gatcgacgcc accacggatg gatcctgtcg
     3121 cggcacagca atcgcgtgct tcaggtatag ctcggtcgat ccgccccagc tttcaccgac
     3181 gtacgacgaa tcctccagca gcacataatc gaaacaggca cgctccaaac tccgcgccat
     3241 atcgacgaac atctccggca ccatccatgt cgtgccgata tgcccggtcc agctttcgcc
     3301 ccaggcctgc acgctcgatc cctgcaggaa ccaaccaagg tggaatggct ttgccgccat
     3361 gtgtcgtttc ccagaagcct tcgcctggac atttcacatg caatcaacgt gccgggcaaa
     3421 tctgctggtt tgtgcggatg gaaatgcaac gcgtgcctat ggattaggcg atcatacgcg
     3481 ctgtgcccgg cccaggtcac cgctagcctc caccaaccgg tgcagcagtt cgatgaacgc
     3541 agcgcgctgc ttcggaggca gcgcggcaac agtacgctcg tgcgcctgct gcgccggacc
     3601 atccatgcgc gccagcatct gctgcccggc cactgtcgca tgcaccagct tccgccgcgc
     3661 atccctttcc acgcgcgtgc gccgcaccag cccgcggcgt tccagccgcg ccagcacatc
     3721 agccatcgtg gtgcgatcga cacccacctg gttggccaac gccgcctgat ccagccccgg
     3781 ctgtgctgcc gcgacagtca ggatgctgta ttgcaccggc gtgacaccga acactttgca
     3841 ctcctcggca aacagcgcga ggtggatctg gtgcagccgc cgcaccaaaa accccggacg
     3901 ctgcccaaga acgcggtaag tttcattcgc aacgaccgcg tccatcagtc caaccccagg
     3961 attgcccgta ctcgtcgcca gatcgcccgc aacatcagcg taccgatggg caggcgcgcc
     4021 ggcgcggaca ccacgcggcc agtgagcctg gcctccaggt tcgctgcgaa tgtcgtcgcg
     4081 atcgacgccg cgaagtcctg tacaacggcg ccccgcgcaa actgtgccag cggcccccgc
     4141 agcgtatact cgatcgtcgc ctcagcccgc gtcgtcgttg cgcccacttc gtgcagccgg
     4201 accactgcgc gcccactcaa tcgggtgcca gagcgaccgt cgcgcccatc gccgctgatc
     4261 tccgcgcgat gctccgccgc atcgaacgcc acctgccctt caccgctgaa tagcgtctcg
     4321 atcggcccca gcgacgcgcg cacttcgccc cgcaattgat cgccctcaac tgagagcagc
     4381 cgagcaccgg gaacg
//
LOCUS       DATGEM010000481         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_695164, whole genome shotgun sequence.
ACCESSION   DATGEM010000481 DATGEM010000000
VERSION     DATGEM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695164"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VKQ27_15110"
     CDS             complement(<1..1277)
                     /locus_tag="VKQ27_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90310.1"
                     /translation="MARLRWSLRGIALIAFSWWFLTGPATAAQLYLPDSPDVPTITLN
                     EAQSGAAGLPPLPVPPPKRWDQQSIWNMQIVGFQDNQGRPSSDDGWVENQNGRYIAYV
                     ANSPGKGLNPLTGKVENNGTTLIDVTDVAHPRLLSHIPTESGGGATHLAVCGGNTLPR
                     AERNHWYLLRHDGQTNQEVWDVTTPEAPRRLTVIISGLTGTHHDWWECDTGIAYTISQ
                     TAVDGWHETGSHQHVYIYDLSNPAQPVFIRQFGLPGQQPDATGAALQSCLNAPGPACY
                     EGITNPPGGVHQLYSAGVRKNRIYAAYGVDADGVFQVLDRQKLLTGCTIPGASPQCAT
                     HPTQADLLYPQISYITTNPNQGGHTAIPIFGVPIPEAQQNYLDGKPQRWDLMATTSED
                     TTNNCISQAWKNPALLDITDDGAPWPIATLAVG"
     gene            complement(1396..2358)
                     /gene="secF"
                     /locus_tag="VKQ27_15115"
     CDS             complement(1396..2358)
                     /gene="secF"
                     /locus_tag="VKQ27_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLY90311.1"
                     /translation="MFMRPTFRLVRDGTKIEFMRGRYMGLIVSAILSTASVILFFYPG
                     LNEGIDFSGGIVMEVRTPGPADFGQIRSALAADNIPEQGVQRFGDPSEVLIRLGQQPS
                     EAAVQQEVNRVRATLEKSIPGVKLLRTDAVGASVSAELFRNGLYALGISLAMIMVYIW
                     FRFEWQFAVGAVVTLVLDITKAIGFLAITRIEFDLVMVAAILTILGYSTNDKVVVYDR
                     VRENLRKYKTMPLRQLIDLSINETLNRTLGTSMTVFLASLPLALFGGQSISGFAWVML
                     FGIVVGTSSSIFIAAPILLFLGEHRLRREGAQAAPAKVGAPTRP"
     gene            complement(2364..>3200)
                     /gene="secD"
                     /locus_tag="VKQ27_15120"
     CDS             complement(2364..>3200)
                     /gene="secD"
                     /locus_tag="VKQ27_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLY90312.1"
                     /translation="DVDGGDLTDARAATNQQTGQWVVTFAFNSLGARRFGDITRANVN
                     HRFAIVLDGKVISAPVIREPITGGRGEISGNFTASSATDLAVLLRAGALPAPLTVVEE
                     RSVGPELGADSIRAGAISLAVGFLLVIGFMATFYGLFGWFANVCLLVNLVLMLGIMSV
                     LQATLTLPGMAGILLTLGMAVDANILINERIREEQRLGRPPLSALEHGFTRAYNTIFD
                     SNATAFLAHVMLFVFGSGPVKGFAVTITVGIATSLFTAWMLTRLLVSRWYVSRRPQLL
                     PV"
BASE COUNT          593 a          889 c         1154 g          564 t
ORIGIN      
        1 tgaccaacag cgagcgttgc gattggccac ggcgcgccgt catcagtgat atcgagcaaa
       61 gcagggttct tccaggcctg gctgatacag ttattggtgg tgtcctccga ggtggttgcc
      121 atcaggtccc agcgctgcgg cttgccgtcg agatagttct gctgcgcttc cgggattggg
      181 acgccgaaga tcggaatggc ggtgtggcca ccctggttcg gattggtcgt gatgtaactg
      241 atctgtgggt agagcagatc agcctgcgtc ggatgcgtgg cacattgcgg cgaggcaccc
      301 ggaatagtgc agccggtgag caacttctgg cggtcgagga cctggaacac gccgtccgcg
      361 tcgacgccat acgcagcgta gatacgattc ttccttacgc cggcggaata cagctggtgg
      421 acaccgccgg gcgggttggt gatgccttcg tagcaggctg ggcccggcgc gttgagacag
      481 ctctgtaagg cggcgcccgt ggcgtcgggc tgctggccag ggagaccgaa ctggcggatg
      541 aataccggct gggctggatt gctgagatca taaatataga cgtgctggtg cgatccggtc
      601 tcatgccagc catcgacggc tgtctgcgag atcgtatagg caatgccggt atcgcactcc
      661 caccagtcat ggtgtgtacc ggtcagtccg ctgatgatga cggtgaggcg cctgggggcc
      721 tcgggggtgg tcacgtccca gacttcctgg ttcgtctggc catcgtggcg caacagatac
      781 caatggtttc gttcagcgcg cggcagggtg ttgcccccac agaccgcgag atgcgtcgcg
      841 ccgccgccgc tttcggtggg aatgtgcgac aaaaggcgcg gatgcgcgac gtcagtaacg
      901 tcgatcagcg tggtgccatt gttctcgacc ttgccggtga gcgggttcag tcccttgccg
      961 gggctgttcg cgacataggc gatgtagcgg ccattctggt tttcgaccca gccatcgtcg
     1021 gagcttgggc gcccctggtt atcctggaaa ccgacgatct gcatgttcca gatcgattgt
     1081 tggtcccagc gctttggtgg cggcacgggc agcggtggca gacctgccgc gccgctctgg
     1141 gcttcgttca gggtgatcgt tgggacgtcg gggctgtctg gtaggtaaag ctgggcggcg
     1201 gtcgcggggc cggtgagaaa ccaccaggag aaggcgatga gggcgatccc acgcagcgac
     1261 caacgcagac gagccattcc cggttcctcc caagtggttg gactcgagtt tatcgctggt
     1321 tggggcgtta taggcaaagc tgggagagtt gccccacgct cgggccggac caccctctac
     1381 accggcgctt tgacgctatg gccgggtggg ggcgccgacc ttggcaggcg cagcctgggc
     1441 gccctcgcgg cgcagccggt gctcgcccag aaacagcagg atcggagcgg cgatgaatat
     1501 cgaggacgag gtgccgacga cgatgccgaa cagcatgacc caggcgaagc cggagatcga
     1561 ttggccgccg aacaatgcga gaggcaggct ggcgaggaag acagtcatcg aggtgccaag
     1621 agtacggttg agggtctcgt tgatcgagag gtcgatcagt tggcgcaggg gcatggtttt
     1681 gtatttacgc aggttctcgc ggacgcggtc gtaaaccacg accttgtcgt tggtggaata
     1741 gccgagaatc gtcaggatgg cggcgaccat caccagatcg aattcgatgc gggtgatggc
     1801 gaggaacccg atggctttgg tgatgtcgag caccagtgtg accacggcac cgacggcgaa
     1861 ttgccattcg aagcggaacc agatgtagac catgatcatc gccaggctga tgccgagcgc
     1921 gtaaaggccg ttgcggaaca gttccgcgga gaccgaagcg ccgacagcgt cggtgcgcag
     1981 aagtttcacg ccggggatgg acttctccaa cgtggcacgg acgcggttca cttcctgctg
     2041 gacggcggcc tcagagggtt gctgccccag gcggatcagg acctcggatg gatcgccgaa
     2101 gcgctgcacg ccctgctcgg ggatgttgtc ggccgcgagg gcggagcgga tctggccgaa
     2161 atcggcgggg ccaggcgttc gcacctccat cacgatgccg ccggagaagt cgatgccctc
     2221 gttcaggcct gggtagaaga acaggatgac ggaggccgtg gacaggattg cggacacgat
     2281 gaggcccatg tagcggcctc gcatgaactc gatctttgtg ccgtcgcgga ccaggcggaa
     2341 ggtggggcgc atgaacatgg cggttacacc ggcaagagct gtggacgccg cgagacatac
     2401 cagcgcgaca caagcaggcg cgtcagcatc caggcggtga aaagcgatgt ggcgatgccc
     2461 acggtgatgg tcactgcgaa gcctttcacc gggccggagc cgaagacaaa cagcatcacg
     2521 tgggcaagga aggctgtcgc gttggagtcg aagatcgtgt tgtaggcgcg ggtgaagccg
     2581 tgctccagcg ccgagagagg cgggcggccg aggcgctgtt cctcgcggat gcgctcgttg
     2641 atcaggatgt tggcgtcaac cgccatgcca agggtgagta ggatgccagc catgccgggg
     2701 agcgtcagcg tcgcctggag cacggacatg atgcccagca tcaggaccag gttcaccagc
     2761 aggcagacgt tcgcgaacca gccgaacagg ccatagaacg tggccatgaa gccgatgacg
     2821 agcaggaagc cgacggcgag cgagatcgcg ccagcgcgga tcgagtcggc gccgagttcg
     2881 ggaccgacgc tgcgctcttc cacgacggtg agcggggccg gcagcgcgcc agcgcggagc
     2941 aggaccgcga gatcggtcgc gctggaggcg gtgaagttgc cgctgatctc gccacgcccg
     3001 ccggtgatcg gctcgcggat gacgggggcg ctgatcacct tgccgtcgag cacgatagcg
     3061 aagcggtggt tgacgttggc acgggtgatg tcgccgaagc ggcgggctcc gagggagttg
     3121 aaggcgaagg tgacgaccca ctgcccggtt tgctgattgg tggcggcgcg ggcgtcagtc
     3181 aggtcgccgc catcgacgtc
//
LOCUS       DATGEM010000482         6471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1382303, whole genome shotgun sequence.
ACCESSION   DATGEM010000482 DATGEM010000000
VERSION     DATGEM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382303"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VKQ27_15125"
     CDS             <1..226
                     /locus_tag="VKQ27_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY90313.1"
                     /translation="IEHHAQRCDIIAAAVQQQPLSVAELVPHVFHRALDEHQTGFAFG
                     EVLAHVNHMLGQGRLSLDIATDGIERYRSI"
     gene            complement(227..1603)
                     /locus_tag="VKQ27_15130"
     CDS             complement(227..1603)
                     /locus_tag="VKQ27_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HLY90314.1"
                     /translation="MANFDAIIIGTGQAGPALARRLAGIGMKVAIVERGRFGGTCINT
                     GCTPTKTLVASAYAAHTARRGAEFGFSGADVKVDMKRVKARKDHVVGLSNHGIERSLK
                     SLENCRVFEGRARFVSPREVQVGEEVLSAGRIFINVGGRAAIPDIPGLDQVPYLTNSS
                     IMDVDFLPRHLLVLGGSYIGLEFGQMYRRFGSEVTIVEIGPRLTPREDQDVSDAIAGF
                     LAKEDVTLRLNATCLRVRNDGGDVVMTVDCDGTSEVRGSHLLIATGRRPNTDDLGLDR
                     AGVNCDARGYITVDGELRTNVPGIWALGDCNGRGAFTHTSWNDYEIAAANLLDGDHRR
                     VSDRITAYALYTDPPLGRCGMTEAEVRKSGRNALIGKIAMEDVSRAFEKSETEGFMKI
                     LVDADSKQILGASFLGMSGDEVIHCVLDTMYAKAPYTMLERAMHIHPTVAEFIPSILQ
                     DLAPLSAP"
     gene            1787..3082
                     /gene="larA"
                     /locus_tag="VKQ27_15135"
     CDS             1787..3082
                     /gene="larA"
                     /locus_tag="VKQ27_15135"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HLY90315.1"
                     /translation="MPDSRIDLLFGKGLLPVSVPEGIRPTVIRKGALPKLPNPPAAIA
                     EALANPIGSASLADLARGRDSACILICDITRPVPNHLFLRPMVETMVTAGIPLAAITI
                     LVATGLHRPNEGEELAELVGDPWVMANVRVENHFARDGAMHIDLGRTTTRGTPITLDR
                     RFVEADLRIATGLVEPHFMAGWSGGRKVIAPGVAGHETIRTFHSARFMGDPLAVQCNL
                     VGNPLHEEQLEIVRRLGPCYALNTIIDEDRHLVYVTFGEVIASHMAAVDFVTGSTKIG
                     MDRRYKTIVTSSAGHPLDKTYYQTVKGMVTPLDILEPGGTLIIASSCSEGFGSAEFRD
                     AQTRLVAMGAERFLATLTAKTLAEIDEWQTQMQMKAMRVGRIELYTTGLDHEERRITG
                     VHVTTDIDMALHHAVVRHNDPDVAFIPEGPYVVPVFAGG"
     gene            3079..4236
                     /locus_tag="VKQ27_15140"
     CDS             3079..4236
                     /locus_tag="VKQ27_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HLY90316.1"
                     /translation="MSLAIRLDAVGGAAGDMFVAAMVDAMPDLRTRVLADASAVLPTG
                     IGTPQFETGTSAGLHCLRFRLMERPHAHHDHAHASFHDMLARIEAAPLSKDTATHATA
                     ILRLLAEAEAAIHHVPVDDVHFHEIGDWDSLMDVTAAGSVAAALDGARWSVSALPRGD
                     GLVRTRHGLLPVPAPATARLLEGFTWRDDGIGGERVTPTGGAIIRHLVPVPTVRVAGR
                     LQATGTGAGTRELPGQPNILRASLYVDTAAQPGEEDILELCFEIDDMTAEELGTATDR
                     LRAVQGVRDLVLVPAIGKKGRPVQTFRLLVDPDQQEFAVAACFTETSTIGLRWQTVHR
                     TVLPRTMSDAGAVRVKTVTRPSGPSRKAESDDLGGIAGLEARRVAKRVAEE"
     gene            4236..5000
                     /locus_tag="VKQ27_15145"
     CDS             4236..5000
                     /locus_tag="VKQ27_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90317.1"
                     /translation="MAPLDTILADMPGLAIAVSGGVDSMTLAHAAHRIGVPGLMVVHA
                     LSPAVPEEATARVRDHAARFAWRLIEVDAGEFTDPDYRANPVNRCYFCKTNLYSRISH
                     VTEGSIASGANLDDLGDYRPGLTAAAEHRVRHPFVEAGMDKSAVRALARSFDLADICE
                     LPAQPCLSSRIETGIAISAADLAFVARMERSLAPHVAAGTDLRCRVTHDGIVVEAATS
                     DQTMQQVAAELCDAEQRRFLGFRPYRRGAAFLHGRP"
     gene            4997..5653
                     /gene="larB"
                     /locus_tag="VKQ27_15150"
     CDS             4997..5653
                     /gene="larB"
                     /locus_tag="VKQ27_15150"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HLY90318.1"
                     /translation="MNDFRLDWGREARTGVAEAVLCEPKTPAQIAAILAEAVENSGRL
                     LLTRLAPEQHDALPAPLRGLLDFDPLSRTAILGGSPSGVPVEGTLILAAGTADLGPAR
                     EAARTLDFHGRAAPLIADVGVAGLWRLNQQLDRLRAARVIIVAAGMEGALFSVVAGLV
                     SAPVIALPTSVGYGVAAGGTVALHAALSSCASGVMAVNIDNGFGAATAAIKILEAGRR
                     "
     gene            complement(5767..>6471)
                     /locus_tag="VKQ27_15155"
     CDS             complement(5767..>6471)
                     /locus_tag="VKQ27_15155"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HLY90319.1"
                     /translation="SGLPGAPAGDMFVACASKSAWHAVGEQIASLILFVNKTYSESGR
                     VQLASIDPDAEPIVDFSLLSDSRDVERLMNGFRRLGAIQQSAALRAVTTDPFPASYSD
                     RVRRIGVVNTRNRIATRVIANLLDGPTALRSWLIDRYVVEGFRFDEVMRDDDRLEAFI
                     RQAAIGVWHASCSCRMGAADDPMAVTDAAGRVRGVDGLRVVDASLFPVVPCANTNIPT
                     LMTAEKIADAILAGGR"
BASE COUNT         1132 a         2235 c         1932 g         1172 t
ORIGIN      
        1 gatcgagcat cacgcccaac gctgcgacat cattgccgcc gcggtccagc agcaaccgct
       61 ctctgtggca gagcttgtgc cgcacgtctt ccatcgcgct ctcgatgagc accagaccgg
      121 ttttgcgttc ggcgaggttc tcgcacacgt caaccatatg ctcggtcagg gacgcctctc
      181 actcgacatc gccactgacg gcatcgaacg ctatcgatca atatgatcaa ggtgcggata
      241 gcggcgcgag gtcctgcagg atcgatggta tgaactccgc caccgtcgga tggatatgca
      301 tcgcgcgctc cagcattgtg taaggcgcct tcgcatacat ggtgtccagc acacaatgga
      361 tcacttcatc cccgctcatg ccgagaaatg acgcacccag gatctgcttg ctgtccgcat
      421 cgaccagtat cttcatgaac ccttcggtct cgctcttctc gaacgcacgc gacacatcct
      481 ccatcgcgat cttgccgatc agagcgttac gcccactctt tcgcacttcc gcctcggtca
      541 tcccacaccg ccccagtggt ggatcggtgt atagcgcata ggcggtgatc cgatcgctga
      601 cccgccgatg atcgccatcc agcagattgg cggcagcgat ctcgtagtcg ttccacgacg
      661 tgtgcgtgaa ggcaccgcgg ccgttgcagt cgccaagcgc ccagatgccg ggcacgttgg
      721 ttcgtagttc gccatcgacg gtgatatagc ctcgcgcatc gcaatttacg cctgcgcgat
      781 ccagtcccag atcgtctgtg ttcggccgcc gtccggtcgc gatcagcaga tgcgatccgc
      841 gtacctccga agtgccgtcg caatcaaccg tcataacgac gtcgccaccg tcgttgcgta
      901 cgcgcaggca agtcgcattc agccgaagcg tgacgtcttc cttcgccagg aaaccagcga
      961 tcgcgtccga cacatcctga tcctcgcgcg gtgtcagccg tggtccgatc tccacgatgg
     1021 tcacctcgct gccgaagcgc cggtacatct gcccgaactc cagtccgata taactgccgc
     1081 ccagcacgag cagatggcgc ggcaggaagt ccacatccat gatcgaactg ttggtgaggt
     1141 aagggacctg atccaggccg ggaatatcgg ggatcgcagc gcgcccacca acattgatga
     1201 agatccgccc ggcactcagg acttcctcgc caacctgcac ctcgcgcggt gaaacgaagc
     1261 gcgcacggcc ttcgaacacg cggcaattct ccaggctctt cagcgaccgc tcgatgccat
     1321 ggttggaaag cccgacgaca tgatccttcc gcgccttcac gcgcttcata tcgaccttca
     1381 cgtcggcgcc actgaacccg aactccgccc cgcgacgcgc cgtgtgagcc gcataggcgc
     1441 tcgccaccag cgtcttcgtc ggcgtgcagc ccgtgttgat gcacgtgccg ccgaaccgac
     1501 cgcgttcgac aatcgccact ttcattccga tgcccgccag ccgtcgcgcg agcgcgggcc
     1561 cggcttgtcc cgtcccaatg atgatcgcgt caaaatttgc catttcctgc tccttggccg
     1621 cgattgtcgg cgtgttgttc aactttcctg caccgggtgc acggacctgt catgaagata
     1681 actgcgtcaa tgcgagctcc tcccgggaaa cgccgtcttc gggaccaaat tgcaccaccc
     1741 cagccatcga gcgcatatag agcctgtcgc ccacggagac tgtccgatgc ctgactcacg
     1801 gatcgacctg ctgttcggca agggcctttt gccggtcagc gtgccggaag gcatccgtcc
     1861 gaccgtcatt cgaaagggcg cgctgccgaa gctgccgaac cctcccgcgg cgatcgccga
     1921 ggcgcttgcc aaccccatcg gctccgcttc gctggccgat ctggcgcgcg gccgagacag
     1981 cgcctgcatc ctgatttgcg acatcacgcg cccagtcccc aatcacctgt tcctgcggcc
     2041 gatggtcgag accatggtga ccgcgggcat cccgctcgcc gcgatcacca tcctggttgc
     2101 cactggcctc catcgtccca atgaaggtga ggaactcgcc gaactggtgg gcgatccctg
     2161 ggtaatggcg aacgtccgcg tcgagaacca cttcgcccgc gacggggcga tgcatatcga
     2221 tctcggccgc accacgacgc gcggcacccc gatcacactc gatcgccgct tcgtcgaggc
     2281 ggacctgcgc atcgccaccg gcctggtcga gccgcacttc atggccggct ggtccggcgg
     2341 ccggaaggtc attgcgcccg gtgtcgccgg ccatgagacg atccgcacct tccactccgc
     2401 ccgattcatg ggtgacccgc tggcggtcca gtgcaacctt gtcggcaatc cgctgcacga
     2461 ggagcaactc gagatcgtgc gccgccttgg cccgtgctac gcgctcaata cgatcatcga
     2521 tgaggatcgc catctggtgt atgtgacgtt cggcgaggtc atcgccagcc acatggccgc
     2581 ggtcgatttc gttacgggct ccacaaagat cggcatggac cgccggtaca agactattgt
     2641 cacgtcgtcg gctggacacc cgctcgacaa gacttactat cagacggtga agggcatggt
     2701 gacgccgctc gacatcctgg aaccaggcgg cacgctgatc atcgcttctt cgtgttcgga
     2761 aggcttcggc tctgccgaat tccgcgacgc acagacgcgg ttggtcgcca tgggcgccga
     2821 gcgcttcctc gccacactca ccgcgaagac gctcgctgag attgacgagt ggcagaccca
     2881 gatgcagatg aaggcgatgc gcgttggtcg cattgaactt tacacgacgg gccttgatca
     2941 cgaagaacgg cggattaccg gcgttcacgt caccaccgat attgacatgg ctctgcatca
     3001 tgccgtcgtg cgccacaacg accccgacgt ggccttcatt cccgagggtc cgtatgtcgt
     3061 tccggtcttc gccggcggat gagcctcgcg atccggctcg atgcagtggg cggcgcggcg
     3121 ggcgacatgt tcgtcgccgc catggtggac gccatgccgg atctccgtac gcgcgttctg
     3181 gcggacgcct ccgccgtgct gccaacgggt atcggcacac cgcagttcga gacgggcacc
     3241 agcgcgggac tgcattgcct gcgcttccgg cttatggaac gcccgcacgc ccatcatgac
     3301 cacgcccacg ccagcttcca cgacatgttg gcgcgcatcg aagccgcacc gttgagcaag
     3361 gacaccgcca ctcatgccac cgccattctg cgcttgctgg ccgaggcgga agcggcgatc
     3421 catcacgtgc cggtggacga cgtgcatttc cacgagatcg gcgactggga ttcactgatg
     3481 gacgtcaccg ctgcgggcag cgtcgccgcg gcgctggatg gcgcgcggtg gagcgtctct
     3541 gccttgccgc gcggtgatgg cctggtgcgc acgcgtcatg gtctgctgcc cgtgccggcg
     3601 cccgccaccg ccagactgct ggaaggcttc acctggcgcg atgatggcat cggcggcgag
     3661 cgtgtcaccc cgaccggcgg cgccattatc cggcatctcg tgcccgttcc gacggtgcgt
     3721 gttgctggac gattgcaagc caccggcaca ggcgccggca cgcgggaact tcccggccag
     3781 cccaacatcc ttcgcgcatc gctctatgtg gatacagcgg cgcagccggg cgaagaggac
     3841 atcctggaac tctgcttcga gatcgatgat atgacagccg aagaactggg caccgcgact
     3901 gatagactgc gtgcggtgca gggcgtgagg gaccttgtgc tggttcccgc catcggcaag
     3961 aaggggcggc ccgtccagac attccgcctg ctcgttgacc cggatcagca ggaattcgcg
     4021 gttgcagcct gcttcaccga gacttccacc atcgggctgc gctggcagac ggtacatcgc
     4081 acggtgttac cacgtacgat gtcggatgca ggcgcggtcc gcgtcaagac ggtgacccgt
     4141 ccttccggcc ccagcagaaa ggcggagagc gatgacctgg gcgggatcgc tggcctggaa
     4201 gcgcgccgcg tcgccaagcg cgtggcggaa gagtgatggc gccgctggat acgatccttg
     4261 ccgacatgcc cggcctggcg atcgccgtca gtggcggcgt ggacagcatg acacttgccc
     4321 atgccgcgca tcgcattggc gtgcctggac tgatggtcgt gcatgcgctg tctcccgccg
     4381 tacccgaaga agccacggca cgggtgcgcg accatgccgc ccgctttgcc tggcgtctga
     4441 ttgaagtgga tgccggcgag ttcaccgatc ccgattatcg tgccaacccg gtgaaccgct
     4501 gctacttctg caaaaccaac ctatacagtc gtatatccca cgtgaccgaa gggtcgatcg
     4561 catcgggcgc caacctcgat gacctgggcg actaccggcc cggccttacc gccgcagcgg
     4621 agcatcgcgt ccgccacccg ttcgtcgagg ccggcatgga caaatccgca gtgcgcgcac
     4681 tcgcgcggtc tttcgacctt gccgatattt gcgaactgcc ggcgcaaccc tgcctctcca
     4741 gccgcatcga gaccggcatc gctatcagcg ctgccgacct ggctttcgtt gctcgcatgg
     4801 agcgctccct ggcgccacac gttgccgcgg gaacggacct gcgctgccgc gtcacccatg
     4861 acggcatcgt cgtcgaagcc gcaacgtcgg accagacgat gcagcaggtt gccgccgagc
     4921 tgtgcgatgc cgaacagcgc cgctttctcg gcttccgtcc ttatcgtcgc ggcgcggcgt
     4981 ttctgcacgg gcgcccgtga acgacttcag gctcgactgg ggacgcgagg cgcgcaccgg
     5041 cgttgcggag gccgtgttgt gcgagcccaa gacgccggca cagatcgccg ccatcctcgc
     5101 cgaagccgtg gagaacagcg gccgtctgct gctgacgcgc cttgctccgg aacagcacga
     5161 tgcgctgccg gcgccgctgc gaggcctgct tgatttcgat ccgctgtcgc gcacggccat
     5221 ccttggcgga tcgccttccg gcgtgccggt cgagggaact ctgatccttg ccgccggcac
     5281 agccgatctt ggtccggcac gcgaggcagc ccgtacgctc gacttccatg ggcgagcagc
     5341 gccactcatt gctgatgtcg gcgtcgccgg gctgtggcgt ctgaaccagc agcttgatcg
     5401 gctgcgcgcg gcacgagtga tcatcgtcgc agccggcatg gagggggcgc tgttcagtgt
     5461 cgtcgccggg ctggtttcgg cgccggtgat cgcgctgccg acgtccgtcg gctatggcgt
     5521 agcagccggc ggcaccgtcg cgttgcatgc cgccctctca agctgcgcct ctggcgtcat
     5581 ggcagtgaac atcgacaacg gcttcggcgc cgccaccgct gcgatcaaga tcctcgaagc
     5641 cggccgccgc tgactcgatc acgccgattg cggttcctgt cattgcgagc cgcaggcgaa
     5701 gcaatcccga tcgagattgc ttcccccggc cttcgccggg gacgcaacga cagcgatagc
     5761 gatagactaa cgtcctccag ccaggatcgc gtccgcaatc ttctccgccg tcattagtgt
     5821 aggtatattc gtattggcgc atggcaccac cggaaacagc gatgcgtcaa ccacgcgcag
     5881 cccatccacg ccccgcacgc gtcccgctgc gtcggtgacc gccatcggat cgtccgcagc
     5941 tcccatccgg caggagcacg acgcatgcca gaccccgatc gccgcctggc ggatgaatgc
     6001 ttctagcctg tcgtcgtcac gcatcacttc atcgaagcga aacccttcga ccacgtagcg
     6061 atcgatcagc cagctccgca acgcggtagg cccgtccagc aggttcgcga tcacccgcgt
     6121 cgcaatgcgg ttcctggtgt tcaccactcc gatgcgacgg acacggtcgc tatagctcgc
     6181 cggaaacgga tccgtcgtca ccgctcgcaa cgcggcgctc tgctggatcg cgccaagacg
     6241 ccggaaccca ttcatcagcc gctccacgtc gcggctatcc gacaacagac tgaaatcaac
     6301 aatcggctca gcatccggat cgatcgaagc aagctgcacg cgccccgact ccgagtacgt
     6361 cttgttgacg aacagaatca gcgacgcgat ctgctcgcca acagcatgcc acgccgactt
     6421 gctggcacag gccacgaaca tgtctccggc cggcgcgcca ggcagccccg a
//
LOCUS       DATGEM010000483         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_508801, whole genome shotgun sequence.
ACCESSION   DATGEM010000483 DATGEM010000000
VERSION     DATGEM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508801"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VKQ27_15160"
     CDS             <1..355
                     /locus_tag="VKQ27_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HLY90320.1"
                     /translation="IAQQDIDLVWTAIALHTTPGVPQHMHPIVSLVTAGVEMDVLGLG
                     YDTFSTAERDAVVRQHPRGDHFKEKIIQAFYDGIHHKPDTTFGNVKADVLADKDPHFR
                     PMNFCRVIRESAWPQ"
     gene            386..559
                     /locus_tag="VKQ27_15165"
                     /pseudo
     CDS             386..559
                     /locus_tag="VKQ27_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
     gene            complement(623..1645)
                     /locus_tag="VKQ27_15170"
     CDS             complement(623..1645)
                     /locus_tag="VKQ27_15170"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HLY90321.1"
                     /translation="MFATSIAGSLPKPAWLAEPNMLWAPWKLSGEELAAAKRDATLLA
                     LRQQEDAGLDIVTDGEQSRQHFVHGFLENVEGIDFGKRIEMGIRNNRYKAMVPTVTGP
                     LRLRGRVHATEARIARAHTTKKLKFTLPGPMTIVDTIADTHYGNRVALAMAFAELLNE
                     EARGLQADGVDVIQFDEPAFNVYMDAVGEWGVAGLERAAEGLTCTTAVHICYGYGIQA
                     NIDWKGTLGSEWRQYETVFPILAKSRIDQVSLECRNSHVPMALMRLLAGKDVLVGVID
                     VATDRVETPDEVADTIAQALSFVPADRLLPCTNCGMAPMRREVAEAKLAALGAGAAMA
                     RKRFAA"
     gene            complement(1684..>2638)
                     /locus_tag="VKQ27_15175"
     CDS             complement(1684..>2638)
                     /locus_tag="VKQ27_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY90322.1"
                     /translation="GFILTSLGRLEAAIAACDAAIRLQPDFAQAQWNQAVAALLAGDF
                     ALGFSKYEWRKRHDRYRRDFIDLPGPQWDGSDAAGRTILVHAEQGFGDTIQFARYLPR
                     IGGSVVLACERPVVPLLAQLPGVDVVPKDAAFPAYDCWIDQMSLPRVFGTRLDTIPSA
                     DGYLHAPACGARLPEGLKVGLAWAGNPAHSNDRRRSMPADALRPVLAIPGISFVSLQV
                     GPRAAELPLLSLPLGDFAATASLVAVLDLVITVDTAVAHVAGALGRPAWVMLPYAPDW
                     RWLLGRSDSPWYNSLRLFRQPTPGDWDTVTKAIATELAKLA"
BASE COUNT          509 a          888 c          778 g          463 t
ORIGIN      
        1 gattgcgcag caggatatcg acctcgtgtg gacggcgatt gctctgcaca cgacgcccgg
       61 cgttccgcag cacatgcacc cgattgtgtc actcgtcacg gcaggcgttg agatggatgt
      121 cctgggtctc ggctatgaca ccttcagcac cgcggagcgt gatgccgtcg tgcgccagca
      181 tccgcgcggc gatcacttca aggagaagat cattcaagcg ttctacgatg gcatccatca
      241 taagcctgac acgaccttcg gtaacgtgaa agcggacgtg ctcgcagaca aggatccgca
      301 cttcaggccg atgaatttct gtcgcgtgat ccgcgagtct gcctggccgc aatgaacggc
      361 atccgcaaca ccgaggaata gacccatgtc atcgccgata cggttcacca acgccaccgg
      421 cgcgccggtt gttgacaata caaacatcat gacagccggc ccacgcgacc ccgccgtgtt
      481 gcaggacatc tggctgatcg agagaatggc gcatttcggt cgcgaagtga tccccgagcg
      541 tcgcaggcat gccaagggtt gaggcgcgta cggcgccggc gttgccaagg ctctgcgtct
      601 gcaggctgaa aggcgctgac ggctacgctg caaaacgctt ccgcgccatg gctgcgcccg
      661 caccgagcgc agccagcttg gcctcggcaa cctcacgccg catcggcgcc atgccgcaat
      721 tggtacacgg caagagccgg tcggccggca cgaacgacag agcctgcgcg atcgtgtcgg
      781 ccacctcgtc gggcgtttcc acgcggtctg tcgccacgtc aatcacgcct accagcacgt
      841 ctttaccggc gagcagccgc atcagcgcca ttggcacatg cgagttgcgg cactccagcg
      901 acacctgatc gatccggctc ttcgccagaa ttggaaatac ggtctcgtac tgccgccatt
      961 ccgagcccag cgtccctttc cagtcgatat tcgcctggat cccatagccg tagcagatat
     1021 gcactgccgt ggtacaggta agcccctccg ccgctcgctc caaccccgcc acaccccact
     1081 cgccaaccgc atccatgtag acattgaaag ctggctcatc gaattggatc acatcgacgc
     1141 catctgcctg cagtccgcgc gcctcctcat tcagcagctc ggcaaatgcc atcgcgagcg
     1201 ccacgcggtt cccatagtgc gtatcggcga tggtatctac gatggtcatt ggccccggca
     1261 acgtgaactt caacttcttc gtggtatgtg cccgcgcgat gcgtgcctcc gtcgcgtgta
     1321 cccgaccgcg cagccgcaat ggtcccgtga ctgtcggtac catcgccttg tagcgattgt
     1381 tgcggatacc catctcgatg cgcttgccga agtcgatgcc ctcgacgttc tcaaggaagc
     1441 catggacgaa gtgctggcgc gactgctcgc catcggtgac gatatcgagc ccagcatctt
     1501 cctgctgcct cagcgccaat agcgttgcgt cacgctttgc cgcggcaagc tcctcgcctg
     1561 atagtttcca gggcgcccac agcatgttcg gttcggcgag ccaggcaggc tttggcaggc
     1621 tgccggcgat actggttgca aacatgcgcc cctcccgatg cgttgcggca tgggtaggcg
     1681 ccgtcatgcg agttttgcaa gctctgtggc aatcgctttc gtcacggtgt cccaatcccc
     1741 tggcgtgggc tgccggaaca ggcgtagcga gttataccag ggcgaatcgg accggcccag
     1801 cagccatcgc cagtctggag cataaggcag catcacccag gccggcctgc ccaacgcacc
     1861 ggcgacatgc gcgaccgccg tgtccacggt gatcaccagg tccaacaccg caaccagcga
     1921 cgcagtagcg gcaaaatcgc ccagcggcag cgataaaagt ggcagttccg ccgcccgcgg
     1981 cccgacctgg agactgacga aactgatgcc cggaatggcg agcacaggcc gcagtgcgtc
     2041 ggctggcatc gatcggcggc gatcattcga gtgtgccggg ttgcccgccc atgccagccc
     2101 aaccttcaac ccttccggca agcgggcgcc gcaggccgga gcgtgaaggt agccgtcggc
     2161 cgacgggatc gtatccagac gcgtaccgaa tacgcgcggc aagctcatct gatcgatcca
     2221 gcagtcgtat gccgggaacg ccgcatcctt tggcacgacg tccacaccgg gcaattgagc
     2281 aaggagcggg accaccggtc gctcgcaggc aagcacgacg ctgccgccga tgcgcggcag
     2341 atagcgggca aactgtatcg tgtcgccaaa accctgttcc gcatgcacca gaatggtgcg
     2401 gcccgccgcg tcgctgccgt cccactgcgg tccgggaagg tcgatgaaat cgcgccgata
     2461 ccgatcgtgc cgcttacgcc attcatattt gctgaagccg agcgcgaagt cgcctgccag
     2521 cagcgccgcg accgcctggt tccactgcgc ctgcgcaaag tcgggctgca gacggatcgc
     2581 cgcatcgcac gcggcaattg ccgcctcgag ccgaccgagc gatgtcagaa tgaagccg
//
LOCUS       DATGEM010000484         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_354608, whole genome shotgun sequence.
ACCESSION   DATGEM010000484 DATGEM010000000
VERSION     DATGEM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354608"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..645
                     /gene="petA"
                     /locus_tag="VKQ27_15180"
     CDS             4..645
                     /gene="petA"
                     /locus_tag="VKQ27_15180"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HLY90323.1"
                     /translation="MSSDVHALGSEPARRDLLKLVTLATAAIGTGAVAWAFIDSMDPA
                     ADVIAAGAPIEVDLSRVSPGQQIIILWRGSPILIINRTPDALKTLQSPAMVQRLSDPN
                     SEAMQQPPYAVNWHRSVNPEYGVMVGICTHLGCLPGYYPQPSKDTPVADWPGGYFCPC
                     HGSKYDLAGRVYSGVPAPYNLPVPPYTFPNPKTLRIGENPAGATFELSAVVQM"
     gene            1090..2271
                     /locus_tag="VKQ27_15185"
     CDS             1090..2271
                     /locus_tag="VKQ27_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90324.1"
                     /translation="MPGHFGTADNDLGAFANTKSNTSFGVFGSNEAPSPAAGGGAGGA
                     GVFGLTVSPGGAGVFGANNGPQGVGVQGNGPDVGVSGFSQGGSGVRGNTRNSGSFGVF
                     GSNEATAAPTGGGAGGAGVFGLTVSPGGAGVFGANNSQRGVGVQGNGATAGVSGFSDH
                     GTGVLGEAGNDAGVTGFHGNPQLQETTVGNDGARAGVFGASDIGAGVVGYSRSPGSFG
                     VIAFGGIRASAINHPTAGEFNGAVQVNGNVQVTGDIFLQGADCAEHFDVIGAAAVEPG
                     TVMVIDDSGALRESTAAYDRKVAGVVSGAGSYRAAVILDRQPGREDRPAVALFGKVYC
                     KVDAGSAPVEVGDLLTSSATPGHAMGVADPARAFGAVIGKALGALRSGKGMIPILLAL
                     Q"
     gene            2279..3229
                     /locus_tag="VKQ27_15190"
     CDS             2279..3229
                     /locus_tag="VKQ27_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90325.1"
                     /translation="MSEPSASTTVIRQLALAADKPVRRRANPRSFGAGIVAEPPLDLH
                     IQGAPRIQTADTIFPPTQPPPIGVSNGGGAIGTGLPVQLIFWGGAWNQPATNPSVATI
                     VAAARSILGGPYMAGLRQYGIRRCGFGGSSVVTSPGPPATYQDDDIRDLVWRLIDSGQ
                     FPEPDDSGGRIIYMVFMPPGTTYGPGGIRGKHLVASDFDFPADIDHAWAGFVLFNGLT
                     TILSTFTHELVEMCTDPESDAWHVTGNQEIGDVCNAVDGPLNGVNVQSYWSAEDNACL
                     IPTAYSVRRALKWSGHVLGGKGLTSIQKPIPSLNKLIVNL"
     gene            3472..3639
                     /locus_tag="VKQ27_15195"
     CDS             3472..3639
                     /locus_tag="VKQ27_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90326.1"
                     /translation="MAEHSTTAAHPLTQVETPDAFLADRQRFWSGFTHFTLGCTIFIV
                     VLLVLMAIFLL"
     gene            complement(3643..4521)
                     /locus_tag="VKQ27_15200"
     CDS             complement(3643..4521)
                     /locus_tag="VKQ27_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY90327.1"
                     /translation="MPNGPFGLEHPLVVTDSIDALAERYRRLGFAPTQRGFHPWGTAN
                     HLILFPDNFIELLGIADHDLLDEPGETGFRFGRFIARALDRSEGVAMVALHSDDIAAD
                     TEALAARGLEHDGLVNFRRAVTLPDGTKDEVAFSLAMLIDEDRPRLSYFLCQHHRPEF
                     LWVPGLMQHPNGAEAITRITCAVGEPFAIWSRFKQIWGEQALTDVDGGFSIATAGGEI
                     LVLDRLTIANRYMQTPLPQGWREEACIIAITVRVRSLFGLHMQLLPANVPFLLMDDVM
                     RIPPSYAGNVILEFAE"
     gene            complement(4610..>6215)
                     /locus_tag="VKQ27_15205"
     CDS             complement(4610..>6215)
                     /locus_tag="VKQ27_15205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLY90328.1"
                     /translation="ILVALPIVYLIALLQSTRAAADHAQLRAAVAEEHARSAAELSRW
                     KHLFDNIAVGIAVSDPKRNVLVTVNTAYAAMHGMTVDAAEGLSIFETYAPLERERIAQ
                     LASSVDQDGFIDFEAYHLRKDGSTFPVRVSLTCVPPHDGMEPQRIVTVTDITAERELQ
                     TRWVDIFEHAAFGISIIEPISNTIGLANPAFAAAYATSVEAIQSTSVFDWCRPAERER
                     IADLNAIADRNGEVDYELEHVRKDGSAFPARVHITSVRTPDGAVRYRIATVQDIAREK
                     QLRVELSQAQRLEAIGQLCAGVAHDFNNLLQAMIAHLEMIEDDVVPETTRDKVDTAIR
                     LAEEGGRLTQQLLSFGRKQLLVPQEVHIQDFLEDFTRLLSHTLDPRIQIELKVASGLA
                     TIFVDPTHLRTALLNLALNARDAMPSGGHLKLEARYASAEGDAILLCTTDTGCGIPDE
                     DLSKVCEPFFSTKGLHGTGLGLPMVHGFVKQSGGDMHISSSPGEGTCVELSLPAQPRQ
                     ERLLHGALPVSIDLGEPGARLQGAPL"
BASE COUNT         1104 a         1889 c         2112 g         1110 t
ORIGIN      
        1 cttatgagtt cagacgtgca tgcgcttggg tcggagcctg cccggcgcga tctgctgaag
       61 ctggtcacgc tggcgaccgc ggcgatcggc acgggcgccg tggcgtgggc gttcatcgat
      121 tcgatggatc cggcggcgga tgtgatcgcg gcgggcgccc cgatagaggt ggacctgagc
      181 cgggtgtcgc cggggcagca gatcatcatc ctgtggcgtg gcagcccgat cctgatcatc
      241 aaccggacgc cggacgcgct gaagacattg cagtcgccgg cgatggtgca gcggctgagc
      301 gatccgaact cggaggcgat gcaacagccg ccctatgcgg tgaactggca ccgctcggtc
      361 aacccggaat acggcgtgat ggtggggatc tgcacgcatc tgggctgcct gccggggtat
      421 tatccgcagc cgagcaagga tacgccggtg gcggactggc cgggcggata tttctgtccc
      481 tgtcatggat cgaaatacga cctggcgggg cgtgtatatt cgggtgtgcc tgcgccgtac
      541 aacctgccgg tgccgccgta cacctttcca aaccccaaga cgctccggat tggtgaaaat
      601 cccgcgggcg cgacgttcga gctgagcgcc gtggtgcaga tgtagctacg ggcggctcgc
      661 ggaccgatgg caggcgggcc gacaaatgac ggcggcatga gcatcggtcg ttctgctttc
      721 caagcgcacc tcgcgaagat cacgactgcg tgagacaact cgaagtcgca tcgatgggac
      781 ggggaataaa tctcgtcggc tgacggtatg ctttgccttc aagcatgtgc cgaatttggg
      841 aaagccgccc tcgaccttag atcgtacgtt gcggatacgg acaggcgtcg ggcggtctgg
      901 cagggccacg ccgtcacggt tgcgtgaggt attggccccg acggaatccc gttacgtagt
      961 tgcaatcaga aaccttttga cttggttaac gagcgcgcac gaaggtccgt cagtcgatgg
     1021 cagccagtcg cttccggaga cggccagaca ggccacgtat ccaggaaatg gcgatcaggg
     1081 aggacccgca tgccaggaca tttcggaacc gcggataacg acctgggggc tttcgccaac
     1141 accaaatcca acacgtcttt cggcgtcttc ggcagcaacg aggcgccgag cccggccgcg
     1201 ggcggtggcg cgggcggcgc gggggtgttc ggactgacgg tctcccccgg tggcgcaggc
     1261 gtgtttggcg ccaacaacgg cccgcagggt gtcggcgtgc agggcaacgg ccccgatgtc
     1321 ggtgtctccg ggttcagcca aggcggcagc ggcgtgcgcg gcaacacccg caacagcggt
     1381 tccttcggcg tgttcgggtc gaacgaggcc acggccgcgc cgaccggtgg cggcgcgggc
     1441 ggcgcggggg tgttcgggct gacggtctcc cccggtggcg caggtgtctt cggtgccaac
     1501 aacagccagc gaggtgtcgg cgtgcagggc aatggcgcca ctgccggcgt ctccggattc
     1561 agcgaccacg gcaccggcgt gctaggtgag gcggggaacg acgcgggcgt taccggattt
     1621 cacggcaacc cgcagcttca ggagaccact gtcgggaacg acggggccag agcgggggta
     1681 ttcggcgcga gcgacatcgg ggccggcgtc gtcggctatt cgaggtcgcc agggtcgttc
     1741 ggcgtgatcg ccttcggggg catccgggca tcggcgatca accatccgac ggcgggcgaa
     1801 ttcaacgggg cggtccaggt caacggcaac gtccaggtca ccggcgacat cttcctgcag
     1861 ggcgcagact gcgccgagca tttcgacgtc atcggggccg cggcggtcga gcccggcacg
     1921 gtgatggtga tcgacgacag cggggcgctc cgggagagta ccgctgccta tgatcggaag
     1981 gttgccggcg tcgtctcggg ggccgggtcg taccgcgcgg cggtcattct ggaccgacag
     2041 cccgggcggg aggatcgacc ggcggtcgcc ctgttcggga aagtctattg caaggtggac
     2101 gccgggtccg cgccagtcga ggtcggcgac ctgctgacga gttcagcgac gccaggccac
     2161 gcgatggggg tggctgaccc tgcgcgggcc ttcggtgccg tcatcggaaa agcgctcgga
     2221 gcgcttcgct cgggcaaggg tatgatcccg atcctcctcg cgctccaata gcggcatcat
     2281 gagcgaacct tccgcatcca caacggtgat ccgccagctc gcactcgcgg cggacaagcc
     2341 ggtgcgccgc cgcgcaaacc cccgcagctt cggcgccggg atcgtcgccg agccccccct
     2401 cgacctgcac atccagggtg ctccgcgtat ccagacggcg gacacgatct tcccgcccac
     2461 gcagccgccg cccatcggcg tttcgaatgg cggcggcgcg atcggcaccg gcctgcccgt
     2521 gcagctgatc ttctggggcg gcgcctggaa tcagcctgcc accaacccct cggtggccac
     2581 catcgtcgcc gccgcgcggt cgatcctggg cggcccctac atggcggggc tgcgtcaata
     2641 cggcatacgc cggtgcggct tcggcggatc cagcgtcgtc accagccccg gcccgccggc
     2701 gacctatcag gacgacgaca tcagggatct cgtctggcgg ctgatcgact ccggccagtt
     2761 tcccgagccc gacgattccg gcggccgtat catctacatg gtgttcatgc cgccgggcac
     2821 gacctacggc ccgggcggca ttcgcggcaa gcacctggtc gcctcggact tcgattttcc
     2881 ggcggacatt gaccacgcct gggcaggatt tgtgctgttc aacggcctga ccacgatcct
     2941 ctccaccttc acccacgagc ttgtcgagat gtgcaccgac ccggagtcgg atgcctggca
     3001 tgtgaccggg aaccaggaga tcggcgacgt ctgcaacgcg gtggacggcc cgctgaacgg
     3061 cgtgaacgtg cagtcctact ggtcggcgga ggacaatgcg tgcctcatcc cgacggccta
     3121 ttcggtgcgg cgggcgttga aatggtcggg ccatgttctg ggcgggaagg gccttacttc
     3181 gatccaaaag ccgattccca gcctcaacaa gctgatcgtg aacctctgac gccgaaccga
     3241 gccaaccatc agactggcgg gcgcctatct caagcctgcc tggcttggaa ggaggctccg
     3301 gatgttgatc gccgatgtgg cgaaaaccaa gggacatgag gtcgtgaagg tgccgccggg
     3361 tgatccggtg gcactcgcgg cggtctggct gcgaagcgga ccgccccttg ccggattctg
     3421 cccggctgcc atagaaccgg ggcagaacaa tccctcctgc cgagaggcat catggccgag
     3481 cacagcacca cagcagcaca cccactcacc caggtggaaa cgccagacgc gttcctcgcc
     3541 gaccggcagc gcttctggag cgggttcacg catttcacgc tgggctgcac gatcttcatc
     3601 gtggttctgc tggtccttat ggcgatcttc ctgctgtagc gtctattcgg cgaattcgag
     3661 gatgacgttg cccgcgtagg atggcgggat gcgcatcacg tcgtccatga gcaggaacgg
     3721 cacgttcgcc ggcaggagct gcatatgcag tccgaacagg ctgcggacgc ggacagtgat
     3781 ggcgatgatg caggcttcct cacgccagcc ctgcggcagc ggcgtttgca tgtagcggtt
     3841 ggcgatggtc agccggtcga ggaccaggat ctcgccgccg gcggtcgcga tgctgaagcc
     3901 gccgtccacg tcggtgagcg cctgttcccc ccagatctgc ttgaagcgtg accagatcgc
     3961 gaaaggctca ccgacggcgc aggtgatccg ggtgatggcc tcggcgccgt tgggatgctg
     4021 catcaatccg gggacccaga ggaattccgg gcggtggtgc tggcagagga aatacgacag
     4081 gcgggggcgg tcctcgtcga tcagcatggc gagcgagaag gcgacctcgt ccttggtgcc
     4141 gtcgggcagg gtgacggcgc ggcggaaatt caccaggccg tcgtgttcaa gaccgcgggc
     4201 tgcgagtgcc tcggtatctg cggcgatgtc gtcgctgtgc agcgcgacca tggcgacgcc
     4261 ctcgctgcga tccagtgccc gggcgatgaa gcggccgaag cggaagccgg tttcaccggg
     4321 ttcgtcgagc aggtcgtggt cggcgatgcc cagcagctcg atgaagttgt cggggaagag
     4381 gatgaggtgg ttggcggtgc cccaggggtg gaagccacgc tgtgtcggcg cgaagccgag
     4441 gcggcggtag cgttcggcca gggcgtcgat gctgtcggtg acgacgagcg ggtgttcgag
     4501 accgaacggg ccgttgggca tggagcgcgc cttctggagg gtgggcgcgg aaggatggct
     4561 tgagcgggag ccgggggcca gagggttatt tgcgaaccgc ctgacaccct cagagtggcg
     4621 ctccttgaag tctggcgccg ggctccccta gatcgataga gaccggtaga gccccatgca
     4681 ggagtctttc ctgcctgggt tgagcaggca aggacagttc cacacaggtc ccctcacctg
     4741 ggctgctgga gatgtgcata tcgccgcctg actgcttgac gaacccgtgg accatcggca
     4801 ggccgaggcc cgtcccatgc aagcctttgg tgctgaaaaa tggttcacag accttgctca
     4861 gatcttcgtc ggggattccg caaccggtat cggtggtaca aagcaaaata gcatcgccct
     4921 cggcgctcgc gtaccgggct tccaatttca aatgaccacc agaaggcatc gcgtcacgcg
     4981 cattgagtgc gagattcagc agcgctgtcc gcaggtgtgt cgggtcgaca aagatcgtcg
     5041 ccaatccgga tgcaaccttc agttcgatct ggatccgagg atcaagggtg tgcgagagca
     5101 agcgagtgaa gtcctccagg aaatcctgaa tatggacctc ctgcgggacc aacagttgct
     5161 ttcggccgaa cgacaggagt tgctgcgtca atctgccacc ttcttcggcg aggcggatcg
     5221 ccgtatcgac tttatcccgg gtggtttccg ggacgacatc gtcctcgatc atttcgagat
     5281 gcgcaatcat ggcctgaagc agattattga aatcatgcgc gacaccggcg catagctgac
     5341 cgatcgcctc caggcgctga gcctggctca gttcgacacg aagctgcttc tcgcgcgcga
     5401 tgtcctggac ggtcgctatt ctgtagcgca ccgccccgtc cggggttcgc acgctggtga
     5461 tatggacgcg ggcaggaaaa gcggaaccgt ccttgcggac atgctccagt tcgtagtcga
     5521 cctctccgtt acgatcggcg atggcattca gatcggcgat gcgctcccgc tccgcaggcc
     5581 tgcaccaatc aaagacggac gtgctttgga tcgcttcgac cgaagtcgcg taagcagcag
     5641 cgaacgccgg gttggcaagg ccgatcgtgt tgctgatggg ctcgatgatg gaaattccaa
     5701 aggcggcatg ctcgaatata tcgacccatc gggtctgcag ctcacgctct gccgttatgt
     5761 cggtcaccgt aactatacgt tgcggttcca tgccgtcatg tggaggcacg catgtaagac
     5821 taactctgac agggaaggtg gagccatctt tccgcagatg ataggcctcg aaatcaatga
     5881 atccgtcctg gtcaacggaa gacgcgagct gagcgatgcg ttcacgctca agcggcgcat
     5941 atgtctcgaa tatggatagt ccttcagcgg catctacggt catgccatgc attgccgcgt
     6001 atgctgtgtt cacagtgacc agcacgttgc gttttgggtc cgagacggcg atcccgaccg
     6061 cgatgttgtc gaagagatgc ttccagcggc tgagctccgc ggccgaacgc gcgtgctctt
     6121 ccgcgactgc cgcacgcagt tgcgcgtggt cggccgcggc acgtgtactt tggagcaaag
     6181 cgatcagata aacgattggc agcgcaacca aaatg
//
LOCUS       DATGEM010000485         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_803340, whole genome shotgun sequence.
ACCESSION   DATGEM010000485 DATGEM010000000
VERSION     DATGEM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803340"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VKQ27_15210"
     CDS             <1..536
                     /locus_tag="VKQ27_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY90329.1"
                     /translation="HIEGHASNENAGMHFGAWRHSRDESPAGGMTGAGVHVLDAFVRL
                     MGPVQSVTAQFVSRKAPPDPTDMLSALFRFRSGATGLFATLRSTPDYRRVHVFGRAGS
                     AEALGDSEVVIRRSGKPVQQLRFTRVDTLRLELEAFADAVEGRARYPITTDEMLDTIG
                     AFEALVTSVATGQSVTC"
     gene            560..1051
                     /locus_tag="VKQ27_15215"
     CDS             560..1051
                     /locus_tag="VKQ27_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HLY90330.1"
                     /translation="MIFGAAVQPGGRPSTTLILRTEAAAVFARRFAEPLFVPTGAVGR
                     HSPSEASVMAALLQSRGVQRERILLEETGVDTLSSARAVVRLLCDQGLTAPVFAASNL
                     YHLPRCLLLLRLLGVSARGAVPPFVPAANRWWRRCYWWLRELPALPYDAALALAIRWR
                     LVD"
     gene            complement(1048..1386)
                     /locus_tag="VKQ27_15220"
     CDS             complement(1048..1386)
                     /locus_tag="VKQ27_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HLY90331.1"
                     /translation="MAKPELGTKRVCVACGTRFYDLTKAPAICPKCGTEQPPDQPRPR
                     RTGGNVAEERRPKKPVPAPGMEDADIEVEGVEEEAEEDILEDASDLEDDADAIGPDIE
                     VEPDSDETER"
     gene            1663..>2103
                     /locus_tag="VKQ27_15225"
     CDS             1663..>2103
                     /locus_tag="VKQ27_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90332.1"
                     /translation="MPIRIVITGTSGAGKTTLARTIAARLALPHIELDAINWQAGWRD
                     LVRYDPPEFIRRVTEAAAAEEWVADGNYGLVRDVLWRRATHMVWLDYQRPVIMRRVIV
                     RSLSRVVMRTELWPGTGNRERWLFLLQSSHPIRWAWNTWLRRRME"
BASE COUNT          312 a          608 c          770 g          413 t
ORIGIN      
        1 tgcatatcga agggcatgcg agcaatgaga atgccgggat gcatttcggt gcgtggaggc
       61 attcgaggga tgagtcgccg gcgggcggca tgacgggcgc cggcgtgcac gtgctggatg
      121 cgttcgtgcg cctcatgggg ccggttcaat cggtgacggc gcaattcgtg agccgcaagg
      181 cgccgcctga cccgaccgat atgctatccg ccttgttccg gttcaggtcg ggcgcgaccg
      241 gcttgttcgc gacactgcgc tccacgccag actatcggcg cgtgcatgtg ttcgggcgtg
      301 caggttcggc ggaggcgttg ggcgatagcg aggtggtgat ccgccgcagc ggcaagccag
      361 tacagcagtt gcggttcacc cgtgtggaca cgttgcggct cgagctggag gcgttcgccg
      421 atgcggtgga ggggcgtgcg cgctatccga tcacgactga tgaaatgctg gacaccatcg
      481 gggcgtttga ggcgttggtg acctcggtgg caactggcca atcggtgacc tgctgatcaa
      541 cgcgcctgac gctgtcattg tgatctttgg ggccgcggtg cagccgggcg ggcgtccaag
      601 cacgacgctg atcttacgca ccgaggcggc agctgtattt gcccgacgct tcgcggagcc
      661 gctgtttgtt cccacgggcg cggtggggcg gcattcgccg tcggaggcct cggtgatggc
      721 agcgctgctg caatcacgag gcgttcagcg agagcgtatc ctgctggagg aaacgggggt
      781 cgatacgctg tcctcggcgc gcgccgtcgt caggctgctg tgcgaccagg ggctcacggc
      841 accggtgttt gcggcgagca acctgtatca cctgccacgc tgcctgttgc tgctgcggct
      901 gctgggggtc agcgcccgcg gcgcagtgcc gccattcgtt ccggctgcca accggtggtg
      961 gcgccgctgc tactggtggc tgcgcgagct gccagctctg ccgtacgatg cagcgctggc
     1021 cctggcgatt cgctggcggc tggtggatta gcgctccgtc tcatcggagt cgggctccac
     1081 ctcgatgtca ggaccgatcg catcggcgtc gtcttcgagg tccgaagcgt cctcgaggat
     1141 gtcctcctcg gcctcttcct cgacgccctc gacctcgatg tcggcgtcct ccatgcccgg
     1201 cgcagggacc ggcttcttgg ggcggcgctc ctcggcgaca ttgcccccgg tgcgccgggg
     1261 acggggctgg tcgggcggct gctcggtgcc gcatttcggg cagatcgctg gggccttggt
     1321 caggtcgtag aagcgggtgc cgcaggcaac gcagacacgc ttggttccaa gttcgggctt
     1381 cgccatccag tggcctcgca ggtccggcgg ttcaagaggc ggcgcccatg ccaccctcgg
     1441 agccgggtgt caattcgggg gcagggcgat gggctcaacg gctattctcc tagtctgccg
     1501 ggaaagatcg gatgatggca tgtttggtgg cggctgtggt gaccaggacg tttgtttttc
     1561 aaacgcggat tgtacattgg aaagtgcggt gaggagttga agcgcttggg tgcagagcgc
     1621 agacgcatcg gtgggacggc tccaggcaac atgcgagcga gagtgccgat acgtatcgtc
     1681 atcaccggca cgtctggtgc aggcaagacg acactggcgc gcactatcgc ggcgcggctg
     1741 gcactgccgc atatcgaact ggacgccatc aactggcagg cggggtggcg cgacctggtg
     1801 cgttacgatc ctccggaatt cattcgtcgc gtcaccgagg cggccgcagc ggaagaatgg
     1861 gtggcggatg gcaactatgg cctggtgcgc gacgtgctgt ggcgccgcgc cacgcatatg
     1921 gtgtggctgg actaccagcg gccagtgata atgcgacggg ttattgtgcg ttccttgtca
     1981 cgcgtggtga tgcgcacgga gttatggcct ggcaccggca accgggaacg atggttgttc
     2041 ctgttgcagt cgagccatcc gattcgctgg gcgtggaata cctggctaag gcggcggatg
     2101 gag
//
LOCUS       DATGEM010000486         9258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1583654, whole genome shotgun sequence.
ACCESSION   DATGEM010000486 DATGEM010000000
VERSION     DATGEM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583654"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="VKQ27_15230"
     CDS             complement(<1..2237)
                     /locus_tag="VKQ27_15230"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HLY90333.1"
                     /translation="MLRTKTFSVAALHNPSHDPLVPDAPFDRAGPIAQELLELTARTT
                     ARAEEDPFGNPVLLVSLAISRRIASGGLDDDMLAAVIRYLRDAAFADRAKRIADYVGG
                     VDVVTHAATLKDLAQRLLRPDPNDSPVRWAEYRALVERARFAAVFTAHPTFALPADVE
                     HALAETASGRPVLTTGSHRAPRITLQEEFEQAGAAIAHGRDAIDQFNAALLVVARGAW
                     PDRWTELTPRPVILSSWVGYDTDGRTDIGWWDTLRLRLEMKRRQLARLQGKVGPGALA
                     DRIATALKAVSHQLALCPDAPQPHRVADFAQALVGRRDVALTSPEPLLPLFAEAIAAA
                     PDDDVRLALCVARAGLVSHGLALAHTHVRLNAAQLHNAVRQRIGIADAPEDPAHRRVL
                     FGAMNAALDTVQPQNVDFGALLVEQASAIRLMMTIAQIIKHVDSSQPIRFLIAETETG
                     YTLLAALWLAKLFGIEKHIEISPLFETASALEHGAEVLEEALRSPHYRAYLQTTGRLA
                     LQFGYSDSGRYVGQLAASYLIERLRLKIGQTLAAHGVTGVEVILFDTHGESVGRGAHP
                     GSLADRLKYLSPTASRQALFRAGLSVREESAFQGGDGYLLFGTPELALATITGIAQQT
                     YHPAAGPIEDPVYANPDFAADFFATVRAGMEALVEDEGYAALLGAFGPSLVDRTGSRP
                     AARQSEGAVGAARIRHPRELRAIPNNAILQQLGWCANTLQGIGAAAARHPELFVDLKE
                     NSRRF"
     gene            2323..3927
                     /locus_tag="VKQ27_15235"
     CDS             2323..3927
                     /locus_tag="VKQ27_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLY90334.1"
                     /translation="MPDDSPLPRPQTTQVGWEPELAELRARIAIAKELGGPDRVERQH
                     KGGRLTVRERIERMLDPGSFHEIGATAGKATYDAESRNITSFMPANCVFGRGTVDGRP
                     VSIAGDDFTLRGGSADASIKGKPKMSEQIAAQFRMPIIRLIEGSGGGGSVKTIETTGR
                     ANLPGGLTETTSFDLLANNMGLVPVVALGLGSVAGLGAARLAASHYSVMVKDISAVFV
                     AGPPVVERLGEPRTKQELGGWEVQLACGAVDHAVDTEDEAFEAARRFLSYLPSSVWDV
                     PPRYRSWDSPDRSEDFLIAAIPREKKQAYHMRRIIEALVDKGSFFEMGAMFGRSIITG
                     FCRLDGWPVALMAGDPLFSGGAWTADACNKIIRFVDMAQTFHMPIVHLVDCPGFQVGL
                     AAESSAVIRWGVRALSAINQTTVPWCSIIVRNCYGVGGLGHQPVSHLCVRYAWPSASW
                     GSLPLEGGIEAAYRAEIDAAPDPQAKMDEIEARLNRLRSPYRTAEPFWVEEVIDPRET
                     RRLLCEFANMAAPLRTPGESRFTMRP"
     gene            complement(3928..5319)
                     /locus_tag="VKQ27_15240"
     CDS             complement(3928..5319)
                     /locus_tag="VKQ27_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HLY90335.1"
                     /translation="MALTARRRGGENGLSAWPGFVDALSTLLMVIIFVLLVFVLAQAF
                     LSVSLSGRDKQLDRVNRQLAEVSDMLSLERSHGSDLQQSINQLSRDLSASNTARSSLA
                     DQLNALKAQAARAGADRDALRADRDRLAQQLADAGLQAQSSAARVDQLQKDVSASAAQ
                     ADSARQEVAQLTTQLADMKKQAAELDKTVQVGKDTIAARLSDLAKLAEQNRALAALRD
                     DLEKQAQDAAVRATTEEQRRAAVQVELAKEKSLGDSATAQIALLNQQVDQLKAQLTSV
                     AQALDVAKSEGAAKDTQIANLGQKLNAALAQKVEELKGYRSEFFGKLRTVLANQPGIQ
                     IVGDRFVIQSEVLFPLGSADLKPAGQNEITALAITIKNIAAEIPADVQWVLRVDGHTD
                     RQPIKAGFAYASNWELSTARAITVVKLLIADGVPPEHLAAAGFADYQPLDPADTPEAY
                     AKNRRIELRLTDR"
     gene            complement(5329..6480)
                     /locus_tag="VKQ27_15245"
     CDS             complement(5329..6480)
                     /locus_tag="VKQ27_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HLY90336.1"
                     /translation="MTRPKVFLIRMLVFLVAVCVVAGVLSNVLLVAFDNNPVLNGVIL
                     AVLLFGIIWNLHQVNRLAPEVVWLETYQTSRARLATLPAPKLLAPMAHMLASRETQTR
                     GDQARFTLSTTAMRSLLDGIASRLDESRELSRYMTGLLIFLGLLGTFWGLLRTIGAVS
                     EVIGSMSVGSGDLNALFEQLKSGLTKPLAGMGTAFSASMFGLSGALVLGFLDLTAGQA
                     QNRFFNELEEWLAGLTRLSSGVLAEGEGSVPVYVQALLEQTAENMENLQHILQRGEDS
                     RGHANQAVMALTDHISMLAETMRTNQQLMLRIAEAQSAIAPALQRMNDYRQTQGFDDA
                     ARAHLRNIEMYLQRLLADAEQGRAQSTAELRNDLRILTRTVAALADDQR"
     gene            complement(6477..7160)
                     /locus_tag="VKQ27_15250"
     CDS             complement(6477..7160)
                     /locus_tag="VKQ27_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLY90337.1"
                     /translation="MDLHALDPLPGSNTQSTKPPPKRFPAPKRITTGKRKAPAAEQPP
                     EQATVAPPATPPASASATPSPPAAPPANLPTTVPPVVALAPVAPSQPAAEPAPPPTPP
                     VSPTAASAATPSGQGLRVTFGAGQSDLSPSSAAAIQDLVKTTAGDGTSYNVVAYAAGT
                     PADPSTARRLSLARALAVRTALMAGGISSSRIYVRALGAEAGDKTPDRVDVAAMGANT
                     SSGEKGAQP"
     gene            complement(7285..8121)
                     /locus_tag="VKQ27_15255"
     CDS             complement(7285..8121)
                     /locus_tag="VKQ27_15255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLY90338.1"
                     /translation="MALRLSPHLTVMANAAQRASRRLLRDFNEVEQLQVSVKGPSDFV
                     SQADVRAEQTLREELNKARPGYAFLMEESGASGSDNWAWRWVVDPLDGTTNFLHGIPH
                     WAISIGLERRLADGTSELAAGLVYAPAVDELFWAEKGGGAFVNERRLRVSARRELKDA
                     LFATGIPFAAVAPHRRLAFARTLGLLMPQVAGIRRFGAAALDLAWVAAGRYDGYWELG
                     LKRWDMAAGLLLVREAGGFANDPSGKDPRDTGDVVAANTHLYPALTEAVVDGIARSQQ
                     GE"
     gene            complement(8123..8689)
                     /gene="efp"
                     /locus_tag="VKQ27_15260"
     CDS             complement(8123..8689)
                     /gene="efp"
                     /locus_tag="VKQ27_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLY90339.1"
                     /translation="MKQQANLIRAGQVIEHEGRRWTVLKQQIITPGKGGAFIQVEMRD
                     LKSGNKTNERWRTADTVERLMTEEKDYNYSYTDGTNLVLMDPESYEEAHIPAELLGDM
                     APFLQDNMQVTVDLVEGDPVGIHLPPTVILEVVEADPVVKGQTAASSYKPAKLSNGVK
                     TSVPPFIETGERIVVKTEDGSYVERAKG"
BASE COUNT         1554 a         3222 c         2869 g         1613 t
ORIGIN      
        1 tggaaccgcc gcgagttctc cttcaagtcg acaaacagct ccggatgccg cgcggccgcc
       61 gccccaatcc cctgcagcgt gttcgcgcac caccccaact gctgcaggat cgcgttgttc
      121 ggaatcgccc gcagctcccg aggatgccgg atgcgtgccg caccgaccgc cccctcggac
      181 tgccgcgccg ccggccggga cccggtacgg tccaccagcg agggcccgaa cgctcccagc
      241 agcgccgcgt agccttcatc ttccaccagc gcttccatcc ccgcgcgcac agtggcgaag
      301 aagtccgcgg caaagtccgg attggcgtaa accggatcct cgatcggccc agctgccgga
      361 tgatacgtct gctgcgcaat cccggtgatc gtcgccagcg ccagctcggg tgtgccgaac
      421 agcaggtagc catcgccgcc ctggaacgcg ctttcctccc ggacgctcag ccccgcacgg
      481 aacagcgcct gccgcgacgc cgtaggcgac agatacttca accgatccgc cagcgatccc
      541 ggatgcgcgc cacgcccgac gctctcgcca tgcgtgtcga acaggatcac ctcgacgccc
      601 gtcacgccgt gcgccgccag cgtctgcccg atcttcagcc gtaatcgctc gatcaggtag
      661 ctcgcagcca actggcccac ataccgcccg gaatccgaat acccgaactg cagcgccagc
      721 cgtcctgtcg tttgtagata cgcgcggtag tgcgggctgc gcagcgcctc ttccagcacc
      781 tcggcgccat gctccagcgc gctcgcggtt tcgaacagcg ggctgatctc gatatgcttc
      841 tcaatgccga acagcttcgc cagccacagc gccgccagca gcgtatatcc ggtctccgtc
      901 tcggcgatca ggaaccgtat cggctgagag ctgtccacat gcttgatgat ctgcgcgatc
      961 gtcatcatca gccggatcgc cgaggcctgc tccaccagca gcgccccgaa atccacgttc
     1021 tgcggctgca cagtatccaa cgccgcattc atcgccccga acagcacgcg ccgatgcgcg
     1081 ggatcctccg gcgcatcggc gatcccgatc cgttgccgca ccgcgttatg cagctgcgcc
     1141 gcattcagcc gcacatgcgt gtgcgccagc gccagcccat gcgacaccag tcccgcgcgt
     1201 gcaacgcata acgccagccg cacatcatca tccggcgcgg cggcgatcgc ctccgcgaac
     1261 agcggcaaca gcggctccgg cgaggtcagc gcgacatccc gccgccccac cagtgcctgc
     1321 gcgaaatccg ccacccgatg cggctgcggc gcatctggac acagcgccag ctggtgcgac
     1381 acagccttca gcgctgtggc gatccgatcc gccagcgccc ccggcccaac cttcccttgc
     1441 agccgcgcaa gctgccgccg cttcatctcc agccgcagcc gcagcgtgtc ccaccagccg
     1501 atatcggtgc gcccatcggt atcgtagcca acccagctcg acaggatcac cggccgagga
     1561 gtgagctctg tccaccgatc tggccacgcg ccccgcgcca ctaccagcag cgcggcgttg
     1621 aactggtcga tcgcgtcacg cccatgcgca atcgccgcgc ccgcctgctc aaactcctcc
     1681 tgcagcgtta tgcgcggggc ccgatgcgac cctgtggtca gtaccggccg gccactcgcg
     1741 gtctccgcca gcgcgtgctc gacatcagcc ggcaaagcaa atgtcggatg agcagtgaac
     1801 accgccgcga atcgggcgcg ttccaccagc gcacgatact ccgcccagcg caccggactg
     1861 tcgttcggat ccggccgtaa caatcgttgt gccaagtctt tcaatgtagc ggcatgtgtc
     1921 actacatcca caccgccaac gtagtcggct atccgcttcg cacggtcggc gaatgcggca
     1981 tcgcgcagat accggatgac cgccgccaac atatcgtcat ccagcccacc cgacgcgatg
     2041 cgacggctga ttgcgagaga taccagcagc acaggattgc cgaacggatc ttcctcggca
     2101 cgtgccgtgg tgcgcgccgt cagctccagg agttcctggg caatcgggcc ggcgcgatcg
     2161 aatggggcat ctggcacaag gggatcatga gacggattgt gcagcgcagc aacagaaaag
     2221 gttttagtac ggagcatgct cggccttctc cgtgttgaac ctttcttgcc acgagcccca
     2281 ttgccccata gcgtccgcaa caaagcccag gaactgtcgc cgatgcccga tgactccccg
     2341 cttccgcgcc ctcagaccac gcaggtgggc tgggaaccgg aactcgctga actgcgcgcc
     2401 cgtatcgcga tcgccaagga actcggcggc cccgaccgcg tcgagcgcca gcacaaaggc
     2461 ggccgcctca ccgttcgcga gcgtatcgag cgaatgctgg acccaggcag cttccacgag
     2521 atcggcgcca ccgccggcaa ggcgacctac gacgccgaaa gccgcaacat caccagcttc
     2581 atgcccgcca actgcgtgtt cggccgcggc accgtcgatg gccgccccgt ctccatcgcc
     2641 ggcgacgact tcacactccg aggtggctcc gccgatgcct ccatcaaagg caagccgaag
     2701 atgtcggagc agatcgccgc gcaatttcgc atgccgatca tccgcctgat cgagggctcc
     2761 ggcggcggcg gctccgtcaa gacgattgaa accacaggcc gcgccaatct ccccggtggc
     2821 ctcaccgaga ccacctcctt cgacctgctc gccaacaaca tgggcctggt cccggtggtg
     2881 gcgctcggcc tcggctcggt tgccggtctc ggcgccgcac gccttgccgc cagccactat
     2941 tcggtgatgg tgaaggacat ctcggccgtc ttcgtggccg gcccgcctgt ggtggagcgc
     3001 ctcggcgaac cacgtacgaa gcaggaactc ggcggctggg aagtccagct cgcctgtggc
     3061 gccgtagatc acgctgtaga tacggaagac gaagcgttcg aagccgcccg ccgcttcctg
     3121 tcttatctgc caagctcggt ctgggacgtg ccgccccgct accgctcctg ggactcaccc
     3181 gatcggagcg aggacttcct gatcgccgcg atcccccgcg agaagaagca ggcctaccac
     3241 atgcgccgca tcattgaagc gctggtcgac aagggcagct ttttcgaaat gggcgctatg
     3301 ttcggccgct ccatcatcac cggcttctgc cgcctcgatg gctggccagt ggcattgatg
     3361 gccggcgacc cgctgttctc cggcggcgca tggacggcgg acgcgtgcaa caagatcatc
     3421 cgcttcgtcg atatggcaca gacgttccac atgccgatcg tccacctggt ggattgcccc
     3481 ggtttccagg ttggcctcgc cgccgagagc agcgcggtga tccgctgggg cgtgcgcgcc
     3541 ctttccgcaa tcaaccagac cacagtcccc tggtgcagca tcatagtccg caactgctat
     3601 ggcgtcggcg gcctcggcca ccagccggtc agccatctgt gcgtgcgcta tgcgtggcca
     3661 tcggcaagct ggggctcgct gccgctggaa ggtggcatcg aagccgccta ccgagccgag
     3721 atcgatgcag cccccgaccc gcaagccaag atggacgaga tcgaggcccg cctgaatcgc
     3781 cttcgctcgc cctaccgcac cgcggagccg ttctgggtgg aagaggtgat cgacccgcgc
     3841 gaaacccgcc gcctgctctg cgagttcgcc aacatggcgg ccccgctgcg cacgccggga
     3901 gagtcgcgat ttaccatgcg gccgtagcta ccgatccgtc agccgcaact cgatccgccg
     3961 gttcttcgca tatgcctcgg gtgtatccgc cggatccagc ggctggtaat cggcgaagcc
     4021 cgcggccgct aggtgctcag gcggcacgcc atcggcgatc agcagcttca ccaccgtgat
     4081 tgcacgcgcg gtcgacagct cccaattcga ggcataggcg aaccctgcct tgataggttg
     4141 gcgatcagtg tgtccgtcca cgcggagaac ccattggacg tcagcgggaa tttccgcggc
     4201 gatgttcttg atcgtgattg cgagtgcagt gatctcattc tggcccgccg gtttcaggtc
     4261 ggcgctgccg agggggaaca gcacctcgct ctgaatgacg aaccggtcgc cgacgatctg
     4321 gataccgggc tgattggcca gtacggtgcg cagcttgccg aagaactcgc tgcgatagcc
     4381 cttcagttcc tcgaccttct gcgccagtgc cgcgttcagc ttctggccca ggttggcgat
     4441 ctgcgtatcc ttcgccgcgc cctccgactt cgcgacgtcc agcgcctgcg ccactgatgt
     4501 cagctgcgcc tttagttgat cgacctgctg gttcagcaac gcaatttgcg ctgtcgccga
     4561 atcacccagc gacttttcct tggccaactc cacctggacc gccgcccgtc gctgctcctc
     4621 ggtggtcgcc cgcaccgctg catcctgcgc ctgcttctcc aggtcatcgc gcagcgccgc
     4681 cagcgcccgg ttctgctccg ccagcttcgc gagatccgac aacctcgcgg cgatcgtgtc
     4741 cttgccgacc tgcaccgtct tgtcgagttc agcggcctgc ttcttcatat cggcaagctg
     4801 cgtggtgagc tgcgccactt cctgtcgcgc cgaatccgcc tgcgccgccg atgccgaaac
     4861 gtccttctgc aactggtcaa cgcgcgccgc gctcgactgt gcctgcaagc cggcgtcggc
     4921 aagctgctgc gccagcctgt cgcgatccgc gcgcagtgcg tcgcgatccg cgccggccct
     4981 cgcggcttgc gctttcaggg cgttgagctg atcggccaat gacgaacgtg ccgtatttga
     5041 ggcggacaag tcacggctga gctggttgat cgactgctgc aggtcggaac catggctgcg
     5101 ttccagcgaa agcatgtcgc tcacttcggc aagctggcgg ttcacccgat caagctgctt
     5161 gtcgcgcccg gacaacgaga ctgacaggaa ggcctgcgcc aggacgaaca ccagcagcac
     5221 gaagatgatc accatcagca gcgtggaaag cgcatccacg aagccgggcc atgccgaaag
     5281 cccgttctcg ccgccgcgcc ggcgtgctgt cagtgccatg gctccgccct atcgctgatc
     5341 atccgccagc gccgcgaccg tgcgcgtcag gatgcgcagg tcgttgcgca gctctgcagt
     5401 gctctgcgcg cgcccctgtt cggcatccgc cagcaggcgt tgtagataca tctcgatgtt
     5461 ccgcaggtgc gctcgcgcgg cgtcgtcgaa cccctgggtc tgccggtagt cgttcatccg
     5521 ctgcagtgcc ggcgcgatcg ccgactgcgc ctcggcgatg cgcagcatca gttgctgatt
     5581 ggtgcgcatc gtctctgcca gcatggagat gtggtccgtc agtgccatca ccgcctggtt
     5641 ggcgtgcccg cggctgtctt cgccacgttg cagtatgtgc tgcaggttct ccatgttctc
     5701 cgcagtctgc tccagcagtg cctgcacata caccggcacc gagccttcac cttccgccag
     5761 cacgccggac gagagccgcg tcagcccggc cagccattcc tccagctcgt tgaagaagcg
     5821 gttctgcgcc tgtcccgcgg tgagatcgag gaaccccagt accagcgcgc cagacagacc
     5881 gaacatcgag gcggagaaag cggtgcccat tcccgccagc ggcttggtca gtccggactt
     5941 gagttgctcg aacagcgcgt tcaggtcacc cgatccaacc gacatcgagc cgatcacttc
     6001 ggacactgcg ccgatggtac gcagcaggcc ccagaatgtg ccgagcagtc ccaggaagat
     6061 cagcagcccg gtcatatagc gcgacaattc gcggctttcg tcgagccgcg aggctatgcc
     6121 atccagcagg ctgcgcatcg ccgttgtgga cagcgtgaag cgcgcctggt cgccgcgcgt
     6181 ctgcgtctca cgcgaggcaa gcatatgagc catcggcgca agcagctttg gcgccggcag
     6241 cgtcgccagc cgcgcgcgtg aagtctggta ggtctccagc cacacgactt ccggcgcgag
     6301 ccgattgacc tggtgcaggt tccagatgat gccgaacagc agcacagcca ggatgacgcc
     6361 gttcagcacc gggttgttgt cgaaggcgac gagcagcacg ttcgacagca cgccggccac
     6421 cacgcacacc gcaaccagga agaccagcat ccggatcagg aagactttcg gccgggtcac
     6481 ggctgtgcgc ccttctcccc ggacgacgtg ttcgcaccca tcgcggcgac gtcgacacga
     6541 tcgggggtct tgtcgcccgc ttcggcgccg agggcacgca cgtagatacg cgacgagctg
     6601 atgccgcccg ccatcagcgc ggtgcgcacc gccagcgcgc gcgccagcga gagacgacgg
     6661 gcggtcgatg gatccgccgg cgtacccgcg gcataggcga caacgttgta gctcgtgcca
     6721 tcacctgccg tcgttttcac caggtcctgg atcgctgccg cactcgacgg gctgaggtcc
     6781 gactgacccg ctccgaacgt cacgcgcagc ccctgaccgc tgggtgtggc cgcgctcgcc
     6841 gccgtgggtg aaacaggcgg tgtcggcgga ggtgccggtt ccgctgccgg ctgcgacggc
     6901 gccaccggcg cgagtgcaac gaccggcggt accgtcgtgg gcagattggc gggcggtgcg
     6961 gccggtgggc ttggagtggc tgaagcggag gcgggcggcg tcgctggtgg tgccacggtt
     7021 gcctgttcag gcggctgttc tgctgccggc gcctttcgct tgccggttgt gatgcgcttt
     7081 ggcgcgggga accgcttggg cggtggcttg gtggattgcg tattgctccc cggcagcggg
     7141 tccagcgcat gcaggtccac tgtgacctgc gcagacgcgg tcgcgctggc gaatgtcagc
     7201 agcggaagta aaatccagac tcgtcgcata cgcgacagac taccggggaa ccgggcgccg
     7261 cgacagtgaa cgtcttggca ggattcattc accttgctgg ctgcgggcga tgccatcaac
     7321 gaccgcctcg gtcagcgccg ggtacagatg cgtgttggcg gcgaccacat caccggtgtc
     7381 gcgcgggtcc ttgcccgagg ggtcgttggc gaagccgccg gcctcccgta cgagcaggag
     7441 gccagccgcc atatcccagc gcttcaggcc gagctcccaa tacccgtcgt agcggccggc
     7501 ggcgacccag gcgaggtcga gcgcagcggc gccgaaccgc cggatgcccg cgacctgcgg
     7561 catcaacaga ccgagcgtcc gggcgaatgc aaggcgtcgg tgcggagcaa ctgcggcgaa
     7621 tgggatgccg gtggcgaaca gcgcgtcctt taactcgcgg cgggcggaaa cgcgcaggcg
     7681 ccgttcattg acgaaggctc ccccgccttt ctctgcccag aacaactcat ccaccgcggg
     7741 cgcgtagacc aagcccgctg caagctcgga cgtcccgtcc gctaggcggc gttccagacc
     7801 gatgctgatc gcccaatgcg gaatgccgtg caggaagttg gtggtgccat cgagcggatc
     7861 caccacccag cgccaggccc agttatcgga accggaagca ccggattctt ccatcaggaa
     7921 ggcatagccg ggacgggcct tgttcaattc ctcgcgcagc gtctgctcgg cgcgcacgtc
     7981 ggcctgcgaa acgaagtcgg atggaccttt gaccgagacc tggagttgct cgacctcatt
     8041 aaagtcacgc agcaggcgcc tggacgccct ctgcgcggcg ttcgccatga cggtgaggtg
     8101 aggggagaga cgaagggcca tttcagccct tggcgcgctc cacgtagctg ccgtcttccg
     8161 tcttcaccac gatcctctcg ccagtctcta tgaatggagg cactgacgtc ttcacgccat
     8221 tcgacagctt ggccggctta taggacgagg ccgccgtctg gcccttgacc acggggtcgg
     8281 cttccacgac ctccagaatg accgtgggtg gcaggtgtat gccgaccgga tccccttcca
     8341 ccagatcgac ggttacctgc atgttgtcct gcaggaacgg tgccatatcg cccagcagtt
     8401 ccgcgggaat atgggcctcc tcgtagctct ccggatccat cagcacgagg ttggtgccgt
     8461 cggtgtagga gtagttgtag tccttctcct cggtcatcag ccgctcgacg gtgtcggcgg
     8521 tgcgccagcg ctcattggtc ttgttgccgg acttcaggtc gcgcatctcc acctggatga
     8581 aggcgccgcc tttgccaggg gtgatgatct gctgcttcag cacggtccaa cgacggccct
     8641 cgtgctcgat gacctgtccg gcacggatca ggttcgcctg ctgcttcatg gtaatagcct
     8701 gccggtctga tttcgaggtt gcgggcttct agaccggggg caggggcggg gcaagccagc
     8761 gggtcagcca cgctctgcgg cggacacccg tgaaagttga tcgagcctgg cgttcccggc
     8821 gccagcactg gtgtgtcgtt ggcgcgttcc ttggctgccg ctcaccgtat cgttgccgtc
     8881 gcctcccagc accgtgttgt tgcagccatc gggattgatc gtgagattcc cgttggccgc
     8941 gggccaggcg aggccggagc ctgtcgcgga gcgggaacag attgcacgac cggtggaggg
     9001 acagatctcc cgcggttgtc gcctgtattc ggtgtcgtgc cgtccatatg aagtctgcct
     9061 ccgcgtcgtg gatcgttgcg gcgcgggaga tagcggcaag cactaccgac gcggtgtgcg
     9121 ctttctcacg ttgcagaaca ggtcgtgccg gcggcctcta tgaaggtggc atcaccggga
     9181 gccgccatgc gagttcggcc acgcggtcca cgatacgaac cggcaacgca ctgtcgatgc
     9241 cgcgcaaatc gatgatct
//
LOCUS       DATGEM010000487         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_71504, whole genome shotgun sequence.
ACCESSION   DATGEM010000487 DATGEM010000000
VERSION     DATGEM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71504"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1192)
                     /locus_tag="VKQ27_15265"
     CDS             complement(176..1192)
                     /locus_tag="VKQ27_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HLY90340.1"
                     /translation="MDGKIHRVPVVTGKHGSKDGAYIGHVDGRPAGNITNFATGYKSN
                     WKSTSTVSALSAEQKATLAAEAAQHRLERARARQHEYERAAQQAEAIWAAATLCTSHP
                     YLAEKNVDAHGVRIAAPGTMLDVGEGEEKRQIDIGGRLLVPVCKDAELRSLQIIDDKG
                     QKMFLKGGEVAGGHHVIGDPNSAWPLHVVEGYATGVTIHANTGHAVVVAFNAYNLAAV
                     AQAYRDRNRDRTIYITGDNDHRKEHETDAQGRQKKNVGREYAVAAAELAGGHALLPKF
                     SANERKLSDWNDLAASRGDEEVRHQLRAGMAIGRRHQLARERDDDQRTPSRSRALERA
                     GIER"
     gene            complement(1305..1859)
                     /gene="traF"
                     /locus_tag="VKQ27_15270"
     CDS             complement(1305..1859)
                     /gene="traF"
                     /locus_tag="VKQ27_15270"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HLY90341.1"
                     /translation="MIRSFVACASAGIAVALTAHGAGVVINATPSMARGVWQRTDQPL
                     ARGVVVIACPPPTATLELGVARHYLADGDCPSRSEPLIKPIAAMPGDTVVVRPSGAIE
                     VNGTLLNNSRSLRNDEGGLPLTGVAACTYTVAPGHVWLISTYSPLSFDSRYLGPVPIS
                     SIRGTMGPQSEGLVRAGRPRPSTI"
     gene            complement(1856..2730)
                     /locus_tag="VKQ27_15275"
                     /pseudo
     CDS             complement(1856..2730)
                     /locus_tag="VKQ27_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
BASE COUNT          459 a          859 c          861 g          551 t
ORIGIN      
        1 cgctgagcgc aaacatgacg accgtggcga tgatcgagag gaacagcccc acaaatccca
       61 gcatcgccgc tgccaccaca ccgagaaact cgatcatcgg agcctctatg caaaaagggc
      121 cgccggtctt cccagtggcc cagatggtat cgcagatgag ctgtcagaac cagatttagc
      181 gttcgatacc agcccgctcc agggcacgac tgcgtgacgg tgtgcgctga tcgtcatcgc
      241 gttccctggc gagctggtgc cgtcgcccga tcgccatacc ggctctcagt tggtgtcgga
      301 cctcctcgtc gccgcgtgaa gctgcaaggt cgttccagtc ggacagcttg cgttcattgg
      361 cactgaactt gggcaggagt gcatgaccgc ccgccagttc ggcggcagcc acggcatatt
      421 cgcgccccac gttcttcttt tgccggccct gcgcatctgt ctcgtgctcc ttgcgatggt
      481 cgttgtcgcc ggtgatgtaa atcgtccggt cgcggttgcg gtcacgataa gcctgcgcga
      541 cggcagcgag gttgtaagcg ttgaatgcca ccacgacggc gtgtccggtg tttgcgtgga
      601 tggtgacgcc agtcgcatat ccctcgacga cgtggagcgg ccaggcgctg ttcgggtcgc
      661 cgatgacgtg gtgaccgccg gccacctccc cgcccttcag aaacatcttc tggcccttgt
      721 cgtcgatgat ctggaggcta cgcagctcgg cgtccttgca gaccgggacc agcaagcgtc
      781 cgccaatgtc gatctgccgc ttctcctccc cctcaccgac atccagcatg gtgccgggcg
      841 ccgcgatccg aacgccatgg gcatcgacgt tcttctcggc gagataggga tgcgatgtgc
      901 agagggtcgc cgcggcccag atggcttctg cctgctgcgc cgcacgctcg tactcgtgct
      961 gccttgccct ggcgcgttcc aggcgatgct gtgccgcctc tgccgccaaa gttgccttct
     1021 gctcggcaga gagcgctgac acggtactgg tgcttttcca gttggactta tagccggtcg
     1081 caaagttggt gatattcccg gctggcctac catccacgtg cccgatgtag gcgccgtcct
     1141 tgctgccgtg cttgccggtg acgaccggaa cgcgatggat cttcccatcc attgccggca
     1201 agccctcaac gatcaggccg acctgttaca gtgcggtctt gaactcaatg cgcggatcgg
     1261 cttccggcgc gatgtagacc ctgcccttcg ttggcaacca cgcctcaaat ggtgctaggt
     1321 cttggcctgc cggcacgaac caggccttcg ctttgcggtc ccatcgtgcc gcgtatgctg
     1381 gagatcggga ccgggcccag ataacggctg tcgaagctga gcggactgta ggtcgagatc
     1441 agccagacat gaccgggtgc gacggtatag gtgcaagccg ccacgccggt cagaggaagt
     1501 ccgccttcgt cgttgcgcag actgcggctg ttgttcaaca gagtgccgtt gacttcgatc
     1561 gccccactcg gccgcaccac gacagtatcg cctggcattg ccgcgatcgg cttgatcaat
     1621 ggctcggaac ggctggggca atcgccatcg gccagatagt gacgagcaac accgagctcg
     1681 agtgtcgccg taggcggagg acaggcgatc accaccacac cgcgcgcaag cggttgatcc
     1741 gtccgctgcc agaccccccg cgccattgat ggcgtcgcgt tgatgacgac accggcacca
     1801 tgcgcggtga gtgccacggc aatgcccgcc gatgcacacg caacaaaaga tcggatcatt
     1861 tcatctcgta ggtcagctgc tgtctggtgg atgtcgcccc cgatggcggc ggcacgctcg
     1921 cccgccattc gaagatcggg tctcgaaagt agaggatctg gcttcctctg atcggcggca
     1981 tgccggccgc gaacagcacc agctcgccgg cctcgacaat ccgatcgcgc tccttcctcg
     2041 gtgcgcggag ccgcatcact tcgtcaggcg tgatcagtgg ccggctggtc tgttgatacg
     2101 agtacgtgac gttggacagg tatcccgatc gcttgccgct ctccgaatag ctatccatga
     2161 ccaccgtggt cgtaccgagc atcttgctga tccactaggc ggtctcgatc ttgtttggcg
     2221 cgtaggcgat ccggatgtgg cagttcgaca tgatcgtctc gtgcgcgtgg taggcggcga
     2281 ggagttgctc gcggtcctgc atcacgagat aggccttgat cccgaacccg gcgcacttcg
     2341 ccaaggcgtc ctcgatcacc gtcatgcggc cgagcgaggg aaactcgtcg agcatgagca
     2401 gcaggcggtg cttgtgtggg atgtgcggct tgccgtcggt gtagcggata tccacaccgg
     2461 tcagtccgcg catgatggtg gtgagcagca ggcggaccag gggccgcagg cgaaccttgt
     2521 ccgagccggg cacgagtatg tagagcgata ccggctttgc gtggttcatc aggtcggcga
     2581 tgcggaagtc gctggtcttc gtgttgcctg cgatcagtgg gtcgcggaac agctcgagat
     2641 acgtcgtcgc ggtgctgtgg accgacgtgc gctctttcgg ctcgcggtta agctggtccc
     2701 ggcctgccgc tgcgacgacg gagtgacgct
//
LOCUS       DATGEM010000488         3771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_735542, whole genome shotgun sequence.
ACCESSION   DATGEM010000488 DATGEM010000000
VERSION     DATGEM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735542"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1588
                     /locus_tag="VKQ27_15280"
     CDS             353..1588
                     /locus_tag="VKQ27_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY90342.1"
                     /translation="MGRPDFIGDLDRSKIVDLDFATAEFRHQAHSLVADWARRPPFYV
                     LGKGPPQVIVGRYAEVHEVFSDTGRFASELPKGAGYEQYDKFMGLTFLTQTDGAQHAR
                     LRRLLIPAFSAHRMEQIESSITEIIEGMLDDIERTGPEFDGMKQYGAKLVIGALVSTM
                     MGLNKEQQQLLVDYQEVVPLATARKPGEAYPAAYESIARRVAELVEFTIAERRAGRRA
                     DFLNDLVQARDRGDKLNDEELFGMIYGLFGAIAATSRSAGGTLYTLYSHTDQLSQLIR
                     DPALIPDAVEECLRIASNGYFTFARIATRDTEIGGTPIYAGMIVRPSPLAANYDPLVY
                     PDPLRFDIHRKPKRIMSFAVGAHFCIGNILGRTTIRIAIERLLARFPKARLADPDFVP
                     VYGGAVGELRLLNLPMVIQ"
     gene            1648..2562
                     /locus_tag="VKQ27_15285"
     CDS             1648..2562
                     /locus_tag="VKQ27_15285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HLY90343.1"
                     /translation="MAAADADYPNRPIRLIVPYAPGGVPDTSARLLARQLQGRIGQSV
                     FVENRPGGNGGIAATVINTSPADGYTLMVVDTTLLSVGPIFLNDLSFNPKKDFVPIAA
                     LARTPLFLAVNAQMPIKTLDEFVAYVRAHPGQLNYGSAGVGSTHQLTMEAMKAALHLD
                     IVHVPYHGTGEAVPALLGNHVQALWAAYPSLKGSVERGDVRLLAHNGLGHSPLLPELP
                     PLADLVPGFDMVTNIGVYGRAGIPEDIVRKIAAECSAVVKERDVASQLALLGMEPVGQ
                     GPETFAKMIQHEIDTITQVVESAGLKPR"
     gene            2977..>3771
                     /locus_tag="VKQ27_15290"
     CDS             2977..>3771
                     /locus_tag="VKQ27_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HLY90344.1"
                     /translation="MAPRLAVAADTIKVGILHSLSGTMAISETTLKDVMLMLIAEQNK
                     QGGLLGKKLEAVVVDPASNWPLFAEKARELISQDKVAAVFGCWTSVSRKSVLPVFKEL
                     DNILFYPVQYEGEESQRNVFYTGAAPNQQAIPAVDYLAGKDGGTVKRWVLEGTDYVYP
                     RTTNQILEAYLKVKKVAPDDIMINYTPFGHSDWQTIVADIKKFASAGKKTAVVSTING
                     DANVPFYKELANQGIKASDIPVVAFSVGEEELAGIDTKNLVGHLAAW"
BASE COUNT          722 a         1194 c         1133 g          722 t
ORIGIN      
        1 cttggaggtg acaggctttt tgcgctccgc cgtggctgct tccgccgtcc cagccatagt
       61 ccaggtcgcg gattgtttgc tactgattac gcgcgcagcg caataacgac gcgccaaatc
      121 gattttcagc attccattta cgtcccgtgc cctttcgaca ggggtgagtt tgtgggtcaa
      181 aaatcaccac tcttcgtaag ccattgatac tatgctacaa aatccgatag agggcggata
      241 ccccctccgc caacgataat gggtttcctg ttctctggct gctaaaatgc ctaacggaag
      301 cgggtctttt tgcggtcctg ctcgaccaag ctttggactc cggagcgacg gcatgggacg
      361 accggatttc atcggcgatc tcgaccgcag caaaattgta gacctcgact ttgcgaccgc
      421 cgagttcagg caccaggcgc attccctcgt cgccgactgg gctaggcggc cgccgtttta
      481 cgtgctcggc aaaggaccgc cccaagtgat cgtcggccgc tatgccgagg tgcacgaggt
      541 tttctcggat accgggcggt ttgcctcgga gctgccgaag ggggcgggtt acgagcaata
      601 cgataaattc atgggcctca ccttcctcac gcagaccgac ggcgcgcagc acgcgcggct
      661 acgtcgacta ctaattccgg cgttctcggc gcatcgcatg gagcagatcg aatcgagcat
      721 cacggagatc atcgaaggca tgctcgacga catcgagcgg accgggccgg aattcgatgg
      781 catgaaacaa tacggcgcca agctcgtgat cggggcgctg gtctcgacca tgatgggatt
      841 gaacaaagag cagcagcagc tgctcgtcga ttatcaggag gtggtgccgc tcgccacggc
      901 aagaaaaccg ggcgaggcct atccggccgc atacgaaagc atcgccaggc gcgtcgccga
      961 gctggtggaa ttcaccatcg ccgagcggcg ggccggccgc cgcgccgatt tcctcaacga
     1021 tctggtccag gcgcgcgacc gcggcgacaa gctgaacgat gaggaattgt tcggcatgat
     1081 ctatggcctg ttcggcgcca tcgccgcgac cagccgatcg gccggcggaa cgctgtacac
     1141 gctgtacagc cacacagatc agctgtcgca gctcattcgc gatccggcgc tgatcccgga
     1201 tgccgtcgag gaatgcctgc gcatcgccag caacggctat ttcaccttcg cgcggatcgc
     1261 cacgcgcgac accgaaatcg gtggaacgcc gatctacgcg ggaatgatcg tgcgtccgtc
     1321 gccgcttgcg gccaactacg atccgttggt ctatcccgat ccgctgcgct tcgacattca
     1381 tcgcaagccg aagcgcatca tgtctttcgc cgtgggggca catttttgca tcggcaacat
     1441 tcttggccgc accacgatta ggattgcgat cgagcgcctg ctcgcccgct ttccgaaagc
     1501 gcggctcgcc gatcccgatt tcgtgccggt ctacggcggc gcggtcggcg aactgcgcct
     1561 gctcaacctg ccgatggtga tccaatgacg cccattacac gccgcgtcgc gcttgtgttg
     1621 ctcggctctg ccgcgtgcca gccccgcttg gctgctgccg atgcggatta tccgaaccgg
     1681 cctatcaggc tgatcgtgcc ttacgcaccc ggcggcgtgc ccgacaccag cgccaggttg
     1741 ctggcccgcc agctgcaggg ccgcatcggc cagtcagttt tcgttgagaa tcgccccggc
     1801 ggcaacggcg gcatcgccgc caccgtgatc aacacgtcgc ccgctgacgg ttatacgctg
     1861 atggtcgtgg acaccaccct gctgtcggtc ggcccgatct tcctcaacga cttgtcgttc
     1921 aatccgaaga aggatttcgt gcccatcgcg gcactggcgc gcacgccgct gttcctggcc
     1981 gtcaacgcac agatgccgat caagacgctc gacgaattcg tcgcatacgt gagggcgcat
     2041 cccggccagc tcaattatgg ctccgcaggt gtcggcagca ctcatcagct gaccatggaa
     2101 gcgatgaagg cggcgctgca tctcgacatc gtccatgtgc cctatcacgg caccggtgag
     2161 gctgtgcccg cgctcctcgg taatcacgtg caggcgctct gggcggccta tccaagcttg
     2221 aagggctccg tcgagcgcgg cgatgtcagg cttctcgctc acaatggtct cgggcattcg
     2281 ccgctattgc cggagctccc gccgctggcc gatctcgttc caggcttcga tatggtcacg
     2341 aacatcggcg tctacggtcg cgccggaata ccggaggaca tcgtccgcaa aattgctgcg
     2401 gaatgcagcg cggtcgtaaa ggaacgcgat gtcgcctcgc aattggcctt gctcggcatg
     2461 gagccggtcg gccaggggcc ggaaaccttc gcgaaaatga tccagcacga gatcgacacc
     2521 atcacccagg tggtcgagag tgccgggctc aagccgcgct gagtcccgaa tcgcgagtgc
     2581 gacttcctga tccgagctac caaggtggtg ttcgcgacgg ctaaaacaga catgcctcac
     2641 ttccgattcg tgccaattac ggacctgcat cggctggaga tcgacgacgg cagttgtcgg
     2701 ctaaggtgag ccttttcggc gagagcttgc cctcttaatg gctgcacgaa aaatgggcgg
     2761 ggagttgctg aaaattcagc cgctgactcg ttcctgctgg aaacaatcct tcagatcagt
     2821 gccttagagc cccggaaaac tggcacggcg cttgcgacct tggccgcaga gcgcccatcc
     2881 gttgcgctcg cccgaccagc gcgaggctca tccatgtccc acaccctcac ccgtcgccgc
     2941 ttgctctctt ccgccaccgg cgttgctgcc ggacttatgg cgcctcggct cgcggtcgca
     3001 gccgatacga tcaaagtcgg cattttgcat tcgctgtccg gcaccatggc gatcagcgag
     3061 accacgctca aggacgtcat gctgatgctg attgccgagc agaacaagca gggcggcctg
     3121 ctcggcaaga agctcgaagc ggtggtggtc gatcccgcct cgaactggcc gttgttcgcc
     3181 gagaaggcgc gcgagctgat cagccaggat aaggtcgccg cggtgttcgg ctgctggacc
     3241 tcggtgtcgc gcaaatcggt gctgccggtg ttcaaggagc tcgacaatat cctgttctat
     3301 ccggtgcaat acgagggcga ggaaagccag cgcaacgtgt tctacaccgg cgcagcgccg
     3361 aaccagcagg caatcccggc ggtcgattat ctggcgggta aggacggcgg cacggtcaag
     3421 cgctgggtgc tggaaggcac cgactacgtc tatccgcgca ccaccaacca gatcctggaa
     3481 gcgtacctga aggtgaagaa ggtcgcgccg gacgacatca tgatcaacta cacgccgttc
     3541 ggtcattccg actggcagac gatcgtcgcc gacatcaaga aattcgcttc ggccggcaag
     3601 aagacggcag tggtctcgac catcaacggc gatgccaacg tgccgttcta caaggagctg
     3661 gcgaaccagg gcatcaaggc ctcggacatc cccgtcgttg cgttctcggt cggtgaggag
     3721 gagctcgccg gcatcgatac caagaacctc gtcggccatc tcgccgcgtg g
//
LOCUS       DATGEM010000489         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_612742, whole genome shotgun sequence.
ACCESSION   DATGEM010000489 DATGEM010000000
VERSION     DATGEM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612742"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1315
                     /locus_tag="VKQ27_15295"
     CDS             194..1315
                     /locus_tag="VKQ27_15295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90345.1"
                     /translation="MTRALNVIDADGHILEPLTLWNDYMDPKHRDAAPKIIRDKDGKQ
                     RLQIGEKLLGSNKGIGAIGGVGARDGTVVDDALEYEQGRKGGFDPHARIPDMDLDGID
                     AAFLYPSIGLFAGAVDDPDVAAAMSRAYNRWLADYCKPYPDRLYGVAMIPMQSVDAAI
                     QEMKFAREKLGFRGMFLRPNPYNGKMIHHPDYEPFWTMAEELDMSVGFHEGGNSGMMQ
                     VGMDRFEERGARHIITHTMEMMLACMAVIWGGVCERHPKIRIAFLESGGGWIAGWLDR
                     MDRHFDDKGFNDSGLKTRPSEIFQRNCWIAFEPVEHCLKPLVDYVGANKILWATDYPH
                     PDGFFPGAPAMVRKLIEGAPQATVNQVMAGGAKGFYALH"
     gene            complement(1400..2680)
                     /locus_tag="VKQ27_15300"
     CDS             complement(1400..2680)
                     /locus_tag="VKQ27_15300"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HLY90346.1"
                     /translation="MWTVGFLVMFFGLLAAGMPIFLVLGVCAAVLFFVSGQPMIGLAQ
                     NMIDQLNSTTLMSLPLFVMAAAFMRRGGVAQALVDLSAAWLGGIKGSLGLVAVVSCAL
                     FAAISGSSVATALAMGTILLPAMLERGYPRGFALGVLGASGTIGVVIPPSLALILYGI
                     VTEQSVPRLFLAGILPGLLQAGAFFVWVLYYARIKNFPAETPLPLKQRLKVTLRAAPA
                     MLVPVVVLVGIYGGFMTITESAALAAVTALIVSVGVYRGFPFGRTLEVIAEALRSAGV
                     IMLIIATALVFGHWMTGSGIPGQLVDYAAHRGFATWEFLLTINVLLLIIGCFLEVVST
                     LLLVMPILAPVLMPLGVDPVHFGIIFTHNMEVGLVHPPVGLNLYVLSTISDAPVGEVI
                     RGILPFLIILLVVLGIITYVPELTLWLPRMVYGS"
     gene            complement(2691..3281)
                     /locus_tag="VKQ27_15305"
     CDS             complement(2691..3281)
                     /locus_tag="VKQ27_15305"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HLY90347.1"
                     /translation="MSAPEATRASGGLARCLAAWDWIERIVVGLLGAFALVIAVLQVF
                     GRYIDPADAITWAEEVIVYVAVWAVMIIASDLVRTNGHVRPDLVLRLMGPQAQRWVES
                     FNCLIALVFTFGMVWYGWDVVNTALLLDQRSSSDLQFPVWIYYLALPVGGGLMFLRYC
                     VRLFMYVFHFDPRVMTIGHSISHEAPVDLTTLPTTE"
     gene            complement(3268..>3513)
                     /locus_tag="VKQ27_15310"
     CDS             complement(3268..>3513)
                     /locus_tag="VKQ27_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90348.1"
                     /translation="KLQEMIQKVWADNLPTWRDNTLKLQANARGVLTKQGVTFVDVPQ
                     AELDRVHADMVKQQDKAVADAHISPKLVELVMSDVGA"
BASE COUNT          784 a         1163 c         1021 g          545 t
ORIGIN      
        1 tgacccgcat aaccagaaga tcagtcactc gacgttcgag gagtacggcc acaactcgac
       61 ccacctgcgc gatcggatgt tgctggcgac cgcgcggttg cttgctgggt ggccgaagat
      121 gccgggcgaa cgcgattgct acgcccgtta catgaaggag tggataacgc actaaaacac
      181 aaggagacgt accatgaccc gagcactcaa cgtcatcgac gccgacggcc atatcctgga
      241 gccattgacg ctctggaatg actacatgga cccgaaacat cgggacgccg cgccgaagat
      301 catccgcgac aaggacggaa aacaacgtct gcagattggc gaaaagttgc tcggcagcaa
      361 taaggggatc ggtgccatcg gcggcgtcgg cgcgcgcgac ggcaccgtgg tggacgatgc
      421 cctcgaatac gaacaaggcc gcaagggcgg gttcgatccg cacgcgcgca tccccgatat
      481 ggacctcgac ggcattgatg cagccttcct ctatcccagc atcggcctgt tcgccggcgc
      541 ggtggacgat ccggacgtcg ccgccgcgat gtcgcgcgcc tacaaccgct ggctcgccga
      601 ttactgcaag ccctatcccg atcggctgta cggcgtcgcg atgatcccga tgcagtccgt
      661 ggacgccgcg atccaggaga tgaagttcgc ccgcgaaaaa cttggcttcc gcggcatgtt
      721 cctgcgacct aatccgtaca acggcaagat gatccatcat ccggactacg agccgttctg
      781 gacgatggcc gaagaactcg acatgtcggt cggtttccac gaaggtggca acagcggcat
      841 gatgcaagtc ggcatggatc gcttcgagga acgcggtgcc cgccacatca tcacccacac
      901 catggaaatg atgctggcct gcatggcggt gatctggggc ggcgtgtgcg agcgtcatcc
      961 caagatccgc atcgccttcc ttgaatcagg cggcggctgg atcgccggct ggctcgatcg
     1021 catggatcgg cattttgacg ataaggggtt caacgactcc ggcctgaaga cgcggccgag
     1081 cgaaatattc cagcgcaact gctggattgc gttcgaaccg gtggaacact gcctgaagcc
     1141 tttggtggat tatgtcgggg caaacaagat cctgtgggcg accgactacc cgcatcccga
     1201 cggcttcttc cccggtgcgc cggcgatggt gcggaagctc atcgagggcg cgcctcaggc
     1261 cacagtcaac caggtgatgg caggcggcgc caagggcttc tacgcgctgc actgatagcg
     1321 gctgtcgttg cgagcggggc gaagcaatct cgtgcgagat tgcttcgtcg ctgcgctcct
     1381 cgcaatgaca ggggggtagc tacgagccgt ataccatccg tggcagccac agcgtcagct
     1441 cgggcacgta ggtgatgatg cccagcacca ccagcaggat gatcaggaac ggcaggatgc
     1501 cgcggatcac ctcgcccacc ggcgcgtcag agatcgtcga cagcacatac aggttcagac
     1561 cgactggtgg atgcaccagc cccacctcca tgttgtgcgt gaagatgatg ccgaaatgca
     1621 cgggatccac accgagcggc atcagcaccg gcgccaggat cggcatcacc agcagcagcg
     1681 tgctgaccac ctccaggaag cagccgatga tcagcagcag aacgttgatc gtgagcagga
     1741 attcccaggt ggcgaagccg cggtgcgctg cataatccac caactgccca ggaatgccgg
     1801 accccgtcat ccagtgcccg aacaccagcg ccgtcgcgat gatcagcatg atcacgccgg
     1861 cactgcgtag cgcctcggcg atcacttcca gcgtgcgacc gaacggaaag ccccgatata
     1921 cgcccacact gacgatcagc gccgtcacag cggccaatgc ggcggattca gtgatcgtca
     1981 tgaagccgcc gtagataccc accagcacca cgaccggtac cagcatcgcg ggtgccgcac
     2041 gcagtgtcac cttcagccgc tgcttcaatg gcagaggcgt ttccgcggga aagttcttga
     2101 tccgcgcgta gtacagaacc cacacgaaaa atgcgcctgc ctgcagcagc cccggcagaa
     2161 tgcccgcaag gaacagccgc ggcaccgact gctcggtgac gatcccatac aggatcagcg
     2221 ccagtgacgg cggaatcacc acgccaatcg tgcccgacgc acccagcacg cccagcgcga
     2281 agccgcgcgg atagccgcgc tccagcatcg ccggcaacag gatcgttccc atcgccagcg
     2341 ccgtagcgac cgaggaaccc gagatcgccg cgaacagcgc gcacgacacc acagccacaa
     2401 ggccgagcga tccctttatc ccaccgagcc acgcggccga cagatcaacc agtgcctgcg
     2461 ccacaccgcc tcgccgcatg aacgccgccg ccatgacgaa cagcggcagc gacatcagcg
     2521 tcgtcgaatt gagctggtcg atcatgttct gcgccaagcc gatcatcggc tggccagaca
     2581 cgaagaacag caccgcggca cacacgccca gcaccaagaa tatcggcatg cctgcggcga
     2641 gcaggccgaa gaacatcacc aggaagccaa cggtccacat ggagcgtgcg ctattcggtc
     2701 gttggcagtg ttgtcagatc gaccggcgcc tcgtgcgaga tcgaatgacc aatggtcatg
     2761 acgcgcggat caaaatggaa cacatacatg aatagccgca cgcaatatcg caggaacatc
     2821 agcccgccgc caaccggcag cgccagataa tagatccaga ccggaaactg cagatcggag
     2881 gaacttcgct ggtccagcag caaagcggtg ttcaccacat cccagccgta ccacaccatc
     2941 ccgaacgtaa agacgagcgc gatcaggcag ttgaacgact ctacccagcg ttgcgcctgc
     3001 ggtcccatca gccgcagcac cagatcggga cgcacgtgcc cgttggtgcg caccagatca
     3061 ctcgcaatga tcatcaccgc ccatacagcg acatagacga tgacttcctc ggcccaggtg
     3121 atcgcatccg ccggatcgat gtagcgtccg aacacctgca aaaccgcgat caccagcgcg
     3181 aaggcaccga gcagcccaac gacgatacgc tcgatccaat cccatgcagc caggcagcgc
     3241 gcgagccccc cgctcgcgcg cgtcgcttca ggcgccgaca tccgacatga ccagctcgac
     3301 cagcttcggc gagatgtgcg cgtcagccac agccttgtcc tgctgcttca ccatgtcggc
     3361 atgcacgcga tccagttcgg cctgcggcac atcgacgaac gtgacgccct gcttggtgag
     3421 cacgccgcgc gcgttggcct gcagcttcaa ggtgttgtcg cgccacgtcg gcagattatc
     3481 cgcccacacc ttctggatca tctcctgcaa ctt
//
LOCUS       DATGEM010000490         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1267752, whole genome shotgun sequence.
ACCESSION   DATGEM010000490 DATGEM010000000
VERSION     DATGEM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267752"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VKQ27_15315"
     CDS             complement(<1..776)
                     /locus_tag="VKQ27_15315"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY90349.1"
                     /translation="MVQIRRMGPQIGVEVTDVDVNTLDDAGFAPIYQAWLDHNVMVVR
                     DQELTIEQFLAYSRRFGLVVPHPSKSTRHPQMPEITLLGINKFDADGKLIDSIYRRGA
                     EGWHTDGAYDAEPFKATQLYALAIPSTGGDTLFANGYAAYNALPLRLKERLDGRLGGF
                     TYGGRRKKRELLNEEDRDWKPVYHPIIRTHPETGRKSLYFDPGKIETVEGVSAQESDA
                     LIDELANYMIQPEAEYRHKWRVGDIVIWDNRCSYHRAAGD"
     gene            888..1934
                     /locus_tag="VKQ27_15320"
     CDS             888..1934
                     /locus_tag="VKQ27_15320"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HLY90350.1"
                     /translation="MHLFQQHAVKDAAAAAQQIPVIDYGPYFAGEPGALARLAEHVRY
                     ACENIGFFYAINHGVPQETIDRGFAASKRFHAMPLDDKLRLQLNQNNIGYMPMNASVQ
                     AASKVHKATKPNQNESFFISHDRGADHPDVLAGLPLRGRNQWPEGMPGLREDMVAYFQ
                     ALGLMCDRMLPAFAVALDMPEDLFAPFFANECHANLRFLHYPPQVDMSENTFGTAPHT
                     DNSFMTALARTDVPGLAIRLPSGEWFPPPIIPGTFLINLGNIMRRWSNDRFLSTPHGV
                     INESGTDRYSIAYFHSPHVNAEIECVPSCVSADNPARYPKAVYRDLVLEFYRANYFHQ
                     QGHKSDAAALAAAD"
     gene            1950..>2481
                     /locus_tag="VKQ27_15325"
     CDS             1950..>2481
                     /locus_tag="VKQ27_15325"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY90351.1"
                     /translation="MKVYEAIAQSLIAEGLTDFFGLMGDGNMWLWGALCRNPAVKPYN
                     ARHESMAVSMADGYARTTGKVGVAMVTCGPGLTQCGTSLIAAYRGKTPIVLVAGEIQA
                     GAKNRTQTMDQRRFVEASSARFHTITSIDNMAEEIAEAFYAARQYRGPVVLNLPMDLQ
                     EDDFDWDYDYRPSTQFL"
BASE COUNT          481 a          745 c          779 g          476 t
ORIGIN      
        1 tagtcgcccg cggcacggtg ataggaacag cggttgtccc agatgacgat atcgccaacc
       61 cgccacttgt ggcggtattc cgcctcgggc tggatcatgt aattcgccag ctcgtcgatc
      121 agcgcgtcgc tttcctgcgc tgaaacgccc tcgaccgtct cgatcttgcc cggatcgaaa
      181 tacagcgact tgcggccggt ttcgggatgg gtgcggatga tcgggtggta gaccggcttc
      241 cagtcgcgat cttcctcgtt cagcagttca cgcttcttcc ttcgcccgcc ataggtgaaa
      301 ccgccgagcc tgccgtccag ccgctccttc agacgcagcg gcagcgcgtt gtaggcggca
      361 tagccgttgg cgaacagagt gtcgccgccg gtactcggga tcgccagagc atagagctgc
      421 gtggccttga atggttcggc gtcataggcg ccgtcggtgt gccagccttc cgcgccccga
      481 cgataaatcg aatcgatcag cttgccgtcg gcatcgaact tgttgatgcc gagcagggtg
      541 atttcgggca tctgcggatg ccgcgtgctc tttgacggat gcggcactac gaggccgaag
      601 cgccgactgt aagcaaggaa ctgttcgatc gtcagttctt gatcgcgcac caccatcacg
      661 ttgtgatcga gccatgcctg atagatgggt gcgaagccag catcatcgag tgtattcaca
      721 tcgacatcgg tgacttccac accgatctgc gggcccatgc gacggatctg aaccatggcg
      781 cgttcctcct tgcgcgcgac ggtagtgaag gggtcagcgg gggtcaaatt ggcggctttg
      841 cgccaccaga cctataatgc acggcgtacc agccagggga gcggttcatg catctgttcc
      901 agcagcacgc ggtgaaggat gccgcggcgg cggcccagca gattccggtg atcgattatg
      961 gcccgtactt cgccggcgaa ccgggtgcgc tggcccggtt ggcggagcac gtgcgttatg
     1021 cctgtgagaa catcgggttc ttctatgcga tcaatcacgg cgtacctcag gaaaccatcg
     1081 atcgcggctt tgcggcgtcg aagcggttcc atgccatgcc gctggacgac aagctgaggc
     1141 tgcagctgaa ccagaacaat atcggctaca tgccgatgaa cgcctcggtg caggcggctt
     1201 caaaggttca caaggcgacg aagccgaacc agaacgagag cttcttcatc agccatgatc
     1261 gcggtgcgga tcatccggac gtgctggccg gattgccgct gcgcggacgc aaccagtggc
     1321 cagaggggat gccgggcctg cgcgaggata tggtggcgta tttccaggcg ctgggcttga
     1381 tgtgcgaccg catgctcccg gcattcgccg tggcgctgga catgccggag gatctcttcg
     1441 caccgttctt cgccaatgaa tgccacgcca acctgcggtt cctgcactac ccgccgcagg
     1501 tggacatgag cgagaacacg ttcggcaccg cgccgcatac cgacaacagc ttcatgaccg
     1561 cgctggcgcg cacggatgtg ccgggcctgg cgatccggct gccaagcggc gaatggttcc
     1621 cgccaccgat catcccgggc acgttcctga tcaacctcgg caacatcatg cgtcgctggt
     1681 caaacgaccg gttcctgtcc acgccgcatg gagtgatcaa cgagtctggc accgaccgct
     1741 attcgatcgc gtatttccat agcccgcacg tgaacgcaga gatcgagtgc gttccgagct
     1801 gtgtatctgc cgacaatcca gctcgctatc cgaaggcggt gtatcgcgac ctggtgctgg
     1861 aattctaccg cgccaactac ttccaccaac aagggcataa atctgatgca gccgctctgg
     1921 ctgctgccga ctgaaggaaa cgaaccacga tgaaagtcta cgaagcgata gcgcagtccc
     1981 tgatcgcaga ggggctgacg gatttttttg gcctgatggg cgacggcaac atgtggctgt
     2041 ggggtgcgct gtgccgcaat cccgcggtga aaccatacaa cgcgcggcac gagtcgatgg
     2101 cggtgtcgat ggccgatggc tatgcgcgca ccaccgggaa ggtcggcgtg gcgatggtga
     2161 cctgtgggcc agggctgacg cagtgcggca cctcgctgat cgcggcgtat cgcggcaaga
     2221 caccgatcgt actggtggcg ggtgaaatcc aggcgggggc gaagaaccgc acgcagacaa
     2281 tggatcagcg ccgcttcgtc gaagcgagca gtgcgcgctt ccacaccatc accagcatcg
     2341 acaacatggc ggaagagatc gccgaagcat tctatgccgc gcgacagtac cgcgggccgg
     2401 tggtgctgaa cctgccgatg gatctgcagg aagatgattt cgattgggac tatgactatc
     2461 gcccatcgac gcagttcctg c
//
LOCUS       DATGEM010000491         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_62575, whole genome shotgun sequence.
ACCESSION   DATGEM010000491 DATGEM010000000
VERSION     DATGEM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62575"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VKQ27_15330"
     CDS             complement(<1..738)
                     /locus_tag="VKQ27_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLY90352.1"
                     /translation="MVDPTITTSEKGIWAIKWSFVVLAITAAIQMGVVWISGSVGLLA
                     DTIHNFGDAATAVPLWVAFVLSRRKASKRFTYGYGRVEDLAGIVIVLIILFSAATAGY
                     ATVQRFLHPQEVDYLWAVAAASIVGFAGNEIAAVIRIKVGKEIDSAALIADGYHARTD
                     GLTSLAVLFGAAGVWLGYPLSDPIIGLLITIAIAQIVWQSSKAIFARMLDGVEPAIID
                     EIRETAIGTKGVEKVADVRARWLGHRLH"
     gene            complement(911..1912)
                     /locus_tag="VKQ27_15335"
     CDS             complement(911..1912)
                     /locus_tag="VKQ27_15335"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HLY90353.1"
                     /translation="MEGKEDSGTGDRRACRRRGPDRFPAHQGLRGRDNAMFLEPSMRL
                     IAGVGSLVIAAALPAAAANAEWDAVAKAIGRPGMEMPGGVYRVAIGRSDLAVTLDGVP
                     IKPALALGSYLAFHKAGKEAMVMGDLVLLPEEVNPVMKKLIEQGIQVTAIHNHLLRAT
                     PQIMYMHYEGHGDAAKLAAAVRTALQESKTPLEAPAKAGAQPAIDLDQAAIEKALGAK
                     ATPNGGVLQFSIARTEAIKEHGMALPPAMGMGIAINFQPTSGGKAAITGDFVLTAKEV
                     NPVLKTLRDNGIEVTALHSHMLDEQPRLFFMHFWANDDAQKLADGLKAALRKVSLQK"
     gene            complement(1825..>2751)
                     /gene="chrA"
                     /locus_tag="VKQ27_15340"
     CDS             complement(1825..>2751)
                     /gene="chrA"
                     /locus_tag="VKQ27_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HLY90354.1"
                     /translation="GLAFIWPSFLMVVALGWLYTLYGGLPWMQAVFYGVGAAVIGIIA
                     LSAYKLTTKSIGKDWLLWAIYVVAAAVTIVTESEIAWIFLISGVLVWLVRAPPKRWLP
                     KGGSASLLAFAPLGNHLPAACAIGTTSDWSVLGQIAVFFGEAGAFVFGSGLAIVPFLY
                     GGVVNEHHWLTERQFVDAVAVAMITPGPVVITVGFIGYLVAGFAGACVAALATFLPCY
                     LFTIIPAPYFRKHGKKPAIVAFVDGCTAAAVGTIAGAVIVLGRRSITDVPTILILGVT
                     LLVLWKAKKIPEPVIVALAAVAGLIVFPLIKG"
BASE COUNT          512 a          906 c          857 g          476 t
ORIGIN      
        1 gtggaggcgg tggccgagcc agcgcgcgcg tacgtcggcg accttttcga cacctttcgt
       61 gcctatcgcg gtctcgcgaa tctcgtcgat gatcgcaggc tcgacgccat cgagcatgcg
      121 cgcaaagatc gccttgcttg actgccacac gatctgcgca atcgcgatgg tgatgagcaa
      181 gccgatgatc ggatccgaca gcggataacc gagccagacg ccggcggcac cgaacaacac
      241 cgccaggctg gtcagcccgt cggtgcgcgc atggtagcca tcggcgatca gcgcagcgct
      301 gtcgatctcc ttgccgacct tgatgcgaat cacggcggcg atttcattcc ccgcgaagcc
      361 gacgatcgat gccgccgcca ctgcccagag gtaatccact tcctgcggat gcaggaatcg
      421 ctggaccgtc gcgtagccgg cagtggcagc actaaagagg atgatgagca cgatgacaat
      481 gccggccagg tcctccacgc gcccgtagcc gtaggtgaag cgcttgctgg cttttctgcg
      541 cgacagcacg aatgccaccc agagcggcac cgcagtcgcc gcgtcgccaa agttgtggat
      601 cgtatcggcg agcaggccca cgcttcccga aatccagacg acgcccatct ggatcgccgc
      661 ggtgatcgcc agcaccacga atgaccactt gatcgcccag atgccttttt cggaagtggt
      721 gatcgtcgga tcgaccacgc cgtgcgtatg cgcatgccgg ccgtgcgaat gcggctttaa
      781 gtcgtcatgg ccgtggtcgt ggtcatgccc atggtggtcg tgctcatcgt gatgccggtg
      841 tcggggttgc ctgaatccga gccaaatcag aaagcggccg aacatgggat ctccggggga
      901 tttgcgccac tcatttctgg aggctgactt tccgcaaggc tgccttcagc ccgtccgcca
      961 gtttttgcgc gtcgtcgttg gcccagaagt gcatgaagaa caggcgcggc tgctcgtcca
     1021 gcatgtggct gtgaagcgcg gtcacctcga tgccgttgtc gcgcagcgtc ttcaggacag
     1081 gattcacctc cttcgccgtc agcacgaagt cgccggtaat agctgccttg ccgccgctgg
     1141 tgggctggaa gttgatggcg atacccatgc ccatggcggg cggcagcgcc atgccgtgct
     1201 ccttgatcgc ttccgtgcgc gcgatactga actgcagcac gccgccgttc ggcgtggcct
     1261 tggcacctag ggccttttcg atcgccgcct gatccaggtc gatcgcgggc tgcgccccgg
     1321 ctttcgcagg cgcctcgagc ggcgtcttgc tctcctgcag cgcagtgcgc acggcggccg
     1381 caagcttggc ggcgtcccca tgcccttcgt aatgcatgta catgatctgc ggcgtcgcgc
     1441 gcagcaggtg gttgtggatc gcggtgacct ggataccctg ctcgataagc ttcttcatca
     1501 ccggattgac ctcttccggc agcagcacga gatcgcccat caccatcgcc tccttgcccg
     1561 ccttgtggaa cgcgaggtac gagccgagcg cgagcgctgg cttgatcggc acgccatcga
     1621 gcgtgaccgc gaggtcgctg cggccgatgg cgacgcgata gacgccgccc ggcatttcca
     1681 tgcccggccg cccaatcgct ttagcgaccg cgtcccattc ggcattggct gcagcggccg
     1741 gcagtgcagc cgcgatgacc agacttccaa ctcctgcgat gagtctcatg cttggctcca
     1801 agaacattgc gttgtccctt cccctcaacc cttgatgagc gggaaaacga tcaggcccgc
     1861 gacggcggca agcgcgacga tcaccggttc cggaatcttc tttgccttcc ataaaacgag
     1921 cagcgtgacg ccgagaatca ggatcgtcgg cacgtcggta atcgagcgcc tgccgagcac
     1981 gatgactgcg cctgcgatcg tgcccacggc cgccgcggta cagccatcga cgaacgccac
     2041 gatcgccggc ttcttgccgt gcttgcgaaa gtaaggcgcc gggatgatgg tgaagaggta
     2101 gcacggcagg aacgtcgcca gggccgcaac gcacgcgccg gcgaagccgg ccaccagata
     2161 gccgatgaag ccgacggtga tgacgacagg tcccggcgtg atcatcgcca ccgccaccgc
     2221 gtcgacgaac tgccgctccg tgagccagtg gtgctcgttc accacgccgc cgtagaggaa
     2281 gggcacgatg gcgaggccgc tgccgaagac aaaggcgccc gcctcgccaa agaacacggc
     2341 aatttgcccg agtaccgacc agtccgacgt cgtgccaatg gcgcaggccg cgggcaggtg
     2401 attacccagc ggagcgaatg cgagcagcga agcgctgccg cccttcggca accagcgctt
     2461 cggtggcgcc ctgaccagcc agacgagcac gcccgagata agaaagatcc acgcgatctc
     2521 ggactctgtc acgatcgtca cggccgcggc gacgacgtag atcgcccaga gcagccagtc
     2581 cttgccgatg ctcttcgtcg tcagcttgta ggcgctcagc gcaatgatgc cgatcaccgc
     2641 cgcgccgacg ccgtagaaca ccgcctgcat ccagggaagg ccaccgtaga gggtatagag
     2701 ccagccgagg gccaccacca tgaggaacga aggccagatg aaggccaatc c
//
LOCUS       DATGEM010000492         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1206813, whole genome shotgun sequence.
ACCESSION   DATGEM010000492 DATGEM010000000
VERSION     DATGEM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206813"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..3130)
                     /locus_tag="VKQ27_15345"
     CDS             complement(17..3130)
                     /locus_tag="VKQ27_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HLY90355.1"
                     /translation="MNPSRPFILRPVATSLMMVAVLLSGIVAYRLLPLSALPEVDYPT
                     IQVVTLYPGASPDVTTSSITAPLERQFGQMPGLNQMSSTSSGGASVITLQFSLALTLD
                     VAEQEVQAAINAATSLLPPDLPSPPIYNKVNPADTPILSLAITSNSLPLPKVQDLIDT
                     RIAQKLSQLPGVGLVSLSGGQRPAVRIRANPKALAAYGMSLDDLRIAIGNANVNQAKG
                     SFDGPSRASTIDANDQLKSAAEYQRLIIAYKNGAAIRLTDVADVIDDAENMRLAAWAN
                     DKAALIMNIQRQPGANVIEVVDRIKKLLPQLQASLPGSVDLVVLTDRTTTIRASVKDV
                     QYELFLAVALVVMVIFLFLRNVAATVIPSVAVPLSLIGTFGVMYLAGFSINNLTLMAL
                     TIATGFVVDDAIVMIENISRYIEEGMQPMEAALKGAEQIGFTIISLTFSLIAVLIPLL
                     FMGDVVGRLFREFAITLAVAILISAFVSLTLTPMMCARLLRHIPENRQSRFYRAGGRF
                     FDVVIERYGRMLDWVLDRQAATLLVAIGTLALTVVLYIQVPKGFFPVQDTGVIQGISE
                     GPQSISFAAMALRQQAVAKVVLQDPAVESLSSFIGIDGANATLNSGRMMINLKPRGVR
                     GIDAVGVIRRLQAQLAQVEGISLYMQPVQDLTIEDRVSRTQYQFTLEDADGNNLGIWV
                     PKLVERLRALPQLADVASDLQDKGLQAYVDIDRSTATRLGVTPAAVDNALYNAFGQRL
                     ISTIFTQSNQYRVVLEVHPDFQNGPASLNDIFITSTDGQQVRLSAVARVIEQSGPLSI
                     SHLGQFPAATISFNLAPGVSLGDAVKAIRATEADLGLPASMQTSFQGAALAFESSLDN
                     TLLLILAAIVTMYIVLGVLYESYIHPVTILSTLPSAGVGALLALMLSRTDLGIIAIIG
                     IILLIGIVKKNAIMMIDFALDAERNEGKSPRAAIHQACLLRFRPILMTTLSALLGALP
                     LMIGTGVGSELRHPLGITMVGGLIFSQVLTLFTTPVIYLAFDRLANRGRRPGKAADEA
                     QAGTVRP"
     gene            complement(3143..>4192)
                     /locus_tag="VKQ27_15350"
     CDS             complement(3143..>4192)
                     /locus_tag="VKQ27_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HLY90356.1"
                     /translation="YLNGLGTVVPLNTVTIKAHVDGELSAVMFREGQMVKQGDLLAQI
                     DPRPYQVQLAQAEGTMARDQALLKNAQADLERYRTLFEQDSIAKQQLDTQASLVRQYE
                     GALKADQAQIDSAKLQLIYSRITAPITGRLGLRQVDVGNIVHASDTNGIVVITQLQPI
                     TVVFTLPEDNVNAVMQKVHAGQKIAVDAYDRAGKIKLATGTLLTVDNQIDTTTGTVKL
                     KAQFANDDAGLFPNQFVNARMLLDVKRDTVLIPSAAILRGTQGTFVYVVKDDKSVTVR
                     PVKVSSTDGETTAIDAGVSAGEMVVVDGTDKLREGAKVELGERNASAGPADGAQQKRG
                     KRHSDGDASSPASGS"
BASE COUNT          753 a         1241 c         1415 g          783 t
ORIGIN      
        1 aagaaatcga ggaatttcac ggccgcacgg tcccggcctg tgcttcatca gcggctttcc
       61 ccggacgtct tcccctgttc gccagccggt cgaaggccag gtagattacc ggcgtcgtaa
      121 acagggtcaa cacctggctg aaaatcaggc cgccgaccat ggtgatgccg agcggatgcc
      181 gcaattccga accgacgccg gtgccgatca tcagcggcag cgcgccgagc agggcggaca
      241 gggtggtcat caggatcggc cggaaacgca gcaggcaggc ctggtggatc gccgcgcgcg
      301 ggctcttgcc ttcattgcgc tccgcgtcga gcgcgaagtc gatcatcatg atcgcgttct
      361 tcttgacgat gccgatcagc aggatgatgc cgatgatggc gatgatgccc aggtcggtgc
      421 gcgagagcat cagcgcgagc agcgcgccca cgcccgcgga gggcagcgtc gacaggatgg
      481 tgactggatg gatgtagctc tcgtagagca cgccgagcac gatgtacatc gtcacgatcg
      541 ccgccaggat cagcagcagc gtgttgtcga gagaagattc gaacgcgagc gccgcgccct
      601 ggaaactggt ctgcatgctg gctggcaggc cgaggtccgc ctcggtcgcg cggatggcct
      661 ttactgcatc gcccagcgag acgccaggtg cgaggttgaa tgaaatcgtc gccgccggaa
      721 actggccgag atggctgatc gagagcgggc cggattgttc gatcacgcgc gcgaccgcgg
      781 acaggcgtac ctgctggccg tcagtggagg tgatgaaaat gtcgttgagc gatgccggcc
      841 cgttctggaa gtccggatgg acctcgagca cgacacgata ctggttcgac tgcgtgaaaa
      901 tggtcgagat caggcgttgg ccgaaagcgt tgtagagcgc attgtccacg gctgccggcg
      961 ttacgcccag ccgggtcgct gtgctgcggt cgatgtccac gtaggcctgc agtcccttgt
     1021 cctgcaggtc gcttgcgaca tcggcgagct gcggcagggc gcgcaggcgc tcgaccagct
     1081 ttggcaccca gatgccgagg ttgttaccgt ccgcgtcttc aagggtgaac tggtactgcg
     1141 tgcggctgac gcggtcctcg atggtcaggt cctgcaccgg ctgcatgtag agcgaaatgc
     1201 cctcgacctg agcgagttgc gcctgcagcc ggcgaatgac gccgaccgca tcgatgccgc
     1261 gcacgccccg tggcttgagg ttgatcatca tgcggccgct gttcagcgtc gcattggcgc
     1321 catcgatgcc gatgaaggac gacaggcttt ccaccgcggg atcctgcagc acgactttcg
     1381 ccacggcctg ctgtcgcagc gccatcgcag cgaaagagat cgactgcggc ccttcggaga
     1441 tgccctggat cacgccggtg tcctgcaccg ggaaaaagcc cttgggcacc tggatataga
     1501 ggaccacggt cagcgcgagc gtgccaatcg ccacaagcag cgtggcggcc tggcggtcca
     1561 gcacccagtc gagcatcctg ccgtagcgtt cgatcaccac gtcgaagaaa cggccgcctg
     1621 cgcgatagaa gcggctctgc cggttttccg gtatgtggcg caataggcgc gcgcacatca
     1681 tcggtgtcag cgtcagcgac acgaatgcgg agatgaggat ggcgactgcc agcgtgatgg
     1741 cgaattcgcg gaacaggcgg ccgacgacgt cgcccatgaa cagcagcggg atcagcacgg
     1801 cgatcagcga gaacgtcagc gagatgatgg tgaagccgat ctgctcggcg cccttgagcg
     1861 cggcctccat cggctgcatg ccttcctcga tgtagcggga gatgttctcg atcatcacga
     1921 tggcatcgtc gacgacgaag ccggtcgcaa tcgtcagcgc catgagcgtg aggttgttga
     1981 tgctgaatcc ggcgagatac atgacaccaa aggtgccgat cagggacagc ggcacggcaa
     2041 cgctcgggat gacggtggcg gccacattgc gcaggaacag gaagatcacc attacgacca
     2101 gggccacggc caggaacagt tcgtactgca cgtccttgac cgaggcgcgg atcgtggtgg
     2161 tgcgatcggt gagcacaacc aggtccaccg agccgggcag ggaggcctgc aactgcggaa
     2221 gaagtttctt gatgcgatcg actacctcga tgacattggc gccgggctgg cgctggatgt
     2281 tcatgatcag cgccgccttg tcgttggccc aggcagccag gcgcatgttc tcggcatcgt
     2341 cgatcacgtc ggcgacgtcg gtcagccgga tcgccgcgcc gttcttgtag gcgatgatca
     2401 ggcgctggta ttcggccgcg gacttcagct ggtcgttggc atcgatggtg gaagcacgcg
     2461 acgggccgtc gaaactcccc ttggcctgat tgacgttggc attgcctatc gcgatgcgca
     2521 ggtcatccag gctcatgccg tatgcggcga gcgctttcgg gttggcccgg atgcgaacgg
     2581 ccggtcgctg gccgccggac aggctgacca gtcccacgcc cggcaactgc gacagcttct
     2641 gcgcgatgcg ggtgtcgatc aggtcctgca ctttgggcag cggcagggag ttcgatgtga
     2701 tcgcgagcga aaggatcggc gtgtcggcgg gattgacctt gttgtagatc ggcggcgacg
     2761 gcaggtcggg cggcagcaga gatgtggccg cgttgattgc ggcctgcact tcctgttcgg
     2821 cgacatcgag cgttagggcg aggctgaatt gcagggtgat gaccgaagcg ccgccggaac
     2881 tggtcgacga catctgattc aatcccggca tctggccgaa ctggcgttcg agcggcgccg
     2941 tgatcgagga ggtcgtcacg tcggggctgg cgccgggata aagtgttacc acctggatgg
     3001 tcggatagtc gacctcgggc agcgccgaaa gcggtagcag gcgataggcg acgatgccgg
     3061 acaacagcac ggcgaccatc atcagcgagg tcgccaccgg gcgcaggatg aagggacgcg
     3121 acggattcat gcgtcgtgcc cgtcagctgc ctgacgcagg cgatgacgcg tcgccgtcac
     3181 tatgccgctt gccgcgcttt tgctgcgcgc cgtcggccgg cccggccgag gcgttgcgtt
     3241 cgccgagttc caccttggcg ccttcgcgca gtttgtcggt gccatcgacg accaccattt
     3301 ccccggcgga taccccggca tcgattgcgg tggtctcgcc gtccgttgaa ctgactttaa
     3361 cgggacgaac ggtgacgctt ttatcgtcct tgaccacgta aacgaaagtg ccctgcgttc
     3421 cgcgcaggat ggctgcggac gggatgagaa cggtgtcgcg ctttacgtcg agcagcatgc
     3481 gcgcgttcac aaactggttc gggaacaggc cagcatcgtc gttggcaaat tgcgccttca
     3541 gcttgaccgt gccagtggtc gtatcgatct gattgtccac ggtcagcagt gtgccggtcg
     3601 cgagcttgat cttgcccgcg cggtcgtaag catcgacggc gattttctga cctgcgtgca
     3661 ccttctgcat cacggcgttg acattgtctt cgggcagggt gaacacgacc gtgatcggtt
     3721 gcagctgcgt gatgacgacg atgccattgg tgtcgctggc atggacgatg ttgccgacat
     3781 cgacctgccg cagcccgagc ctcccggtga tcggcgcggt gatgcgcgaa taaatcaatt
     3841 gcagcttggc gctgtcgatc tgggcctggt ccgccttgag cgcaccctcg tactgacgca
     3901 ccagcgaggc ctgtgtgtcg agttgctgtt tggcgatgga atcctgctcg aacagcgtgc
     3961 gataacgctc caggtcggcc tgggcgttct tcaacagcgc ctggtcgcgc gccatcgtgc
     4021 cttcggcctg ggcgagctgg acctgatagg gacgggggtc gatctgcgca agcaggtcgc
     4081 cttgcttgac catctggccc tcgcggaaca tgaccgcgct caattcgccg tcgacatgcg
     4141 ccttgatcgt gacggtgttg aggggaacga ccgtgcccag accgttcaga ta
//
LOCUS       DATGEM010000493         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_480885, whole genome shotgun sequence.
ACCESSION   DATGEM010000493 DATGEM010000000
VERSION     DATGEM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480885"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1100)
                     /locus_tag="VKQ27_15355"
     CDS             complement(186..1100)
                     /locus_tag="VKQ27_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90357.1"
                     /translation="MAGMMEGKVAVVTGSGGGIGREIAIAMAAAGAKVIVADIGASLS
                     GEGGSATPAQQTRQLIEQKGGAAEVCTESVAEWGSAQKIIQAALDHYGRIDAVVNNAG
                     ILRDVIFHKMTPEDWLAVIAVHLNGSFFVSRAAAEHYRKQESGTFVHISSTSGLIGNF
                     GQANYSAAKLGITALSKSIALDMQRYNVRSNCIAPFAWSRMTQSIPATTPEQHARLAK
                     IQQMTPDKNAPLAVYLSSDAAKDVSGQVFGTRMNEIILFNSPRPTRIVHRAEGWTPET
                     IAEHAAPALKAGFMPLDRSGEVFSWDPL"
     gene            complement(1147..>3239)
                     /locus_tag="VKQ27_15360"
     CDS             complement(1147..>3239)
                     /locus_tag="VKQ27_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HLY90358.1"
                     /translation="LGIDAATCDRLRRMQPVVYAALPGIADRFYAYMGQQRELAHLLG
                     GPDRIAHLKKTQIEHWHELFQGTFTLAYFQRAVAIGHAHDRIGLEPRWYTGAYALILA
                     DLIAAVAAKSRAKDLPNDIAAVLRAAFLDMDLAVSTYIQAGEANRMRREMLAMSEVLD
                     REMALSAAEITTKAERLSEMAEQLGEVAEQVRTMAEAVSTSVETTAQNVQTVASATQQ
                     LEASSREITGHVGRTSSMTQQAVQQVAATGDAVKSLSSASGKIADVVVLVRSIAGQTK
                     LLALNATIEAARAGDAGKGFAVVAGEVKQLAAQTEDAIGNVNAQAHSIADATNSAATM
                     VGQIANQVHAVDAIAAEVSAATDQQRDATAEIMRNVTLAAEHIGSVASNAHELHTQAQ
                     TTDTTARQFLALAKSVSTEIHDLHHRLAVILRASDAGNRRQSLREPVALGFSFTAAGT
                     PATGFTADLSPHGTLLVAEVPETLAGQKLTVMLDRIGKIDGVIRAVSPLGVHFQFNAN
                     DAATEAAIARVLEESRHADQTYVTLCQAVAAETARTFEAALSDARITEKALFDNHYDA
                     IPDTDPQQHISASTNICEALVPAIIERAKRTDQRIVFCIPSDRNGYIAVHHPEVSQKQ
                     RPGDRIWNIANCRNRRIFDDRTAILAARNPNPILVQTYQRDLGHSKVVMKEFDSPITV
                     RGQQWGAVRLAIRP"
BASE COUNT          501 a          896 c         1211 g          631 t
ORIGIN      
        1 gatcaacaga tcctaggtgc cgcgcagtcc ctgaccccgc gcaggggtga aactgcgatg
       61 gcacgcgtga gtcggatacg tctcattgaa atactacgtg gttccgatgc ggctgccgcc
      121 gcgatgcaag cctcgctcta gcggtttcct cgtgggagct gccagcaggg cttgtcagga
      181 gaatgtcaaa gcgggtccca gctgaagact tcgccggagc ggtcgagggg catgaagccg
      241 gctttcaggg ctggggcggc gtgttcggcg atcgtctcgg gggtccagcc ttcggcgcgg
      301 tgcacgatgc gggtggggcg gggagagttg aacaggatga tctcgttcat gcgggtgccg
      361 aagacctggc cggagacatc cttcgcggca tcggatgaca gatataccgc gaggggcgca
      421 ttcttgtcgg gggtcatctg ctggatcttg gccaggcgcg cgtgctgctc gggcgtcgtg
      481 gcgggaattg actgggtcat gcggctccag gcgaagggag cgatgcaatt ggaccgcaca
      541 ttgtagcgct gcatgtcgag tgcgatcgac ttggagagcg cggtgatgcc gagcttggcg
      601 gcggagtagt tggcctggcc gaaattgccg atcagaccgg aggtgctgga gatgtggacg
      661 aaggtgccgc tctcctgctt gcggtagtgc tcggcggcag cacgggagac gaagaagctg
      721 ccgttgaggt gtacggcgat gacggccagc caatcctcgg gggtcatctt gtggaagatc
      781 acgtcgcgga ggatgccggc attgttcacc accgcgtcga tacggccgta gtgatcgagc
      841 gccgcctgga tgatcttctg agcggagccc cattcggcga cggattccgt gcagacctca
      901 gcggcgccac ccttctgctc gataagctgc ctggtctgct gggcgggggt ggccgaacct
      961 ccttcgccgg agagcgaggc gccgatatcg gcaacgatga ccttggcgcc ggcagcggcc
     1021 atggcgattg cgatctcgcg gccgatgccg ccgcctgagc cggtaacgac tgcgaccttg
     1081 ccttccatca tgcccgccat ggggttcctc cgacgcggtg aaactgccgg cgggtattag
     1141 gcggcactac gggcggatgg caaggcgcac cgcgccccat tgctggccgc ggaccgtaat
     1201 gggcgagtcg aattccttca tgacgacttt gctgtggccc agatcgcgtt ggtatgtctg
     1261 caccaggatt gggttgggat tgcgggcagc gaggatggcg gtgcggtcgt cgaaaatgcg
     1321 gcggttgcgg cagttggcga tattccagat ccggtcgccg ggacgctgct tctggctgac
     1381 ttccgggtga tggacggcga tgtagccgtt ccggtcggac gggatgcaga atacgatgcg
     1441 ctggtcggtg cgcttggcgc gttcgataat ggctggcacc agcgcttcgc agatgttcgt
     1501 gctcgccgag atgtgctgct ggggatcggt atccgggatg gcgtcgtagt ggttgtcgaa
     1561 cagcgccttc tcggtgatgc gggcatctga gagggcagcc tcgaaggtcc gcgcggtttc
     1621 ggcggcgacg gcctggcaaa gcgtgacata ggtctgatcg gcgtggcgcg actcttccag
     1681 aacgcgggcg attgctgcct cggtcgcggc gtcgttcgcg ttgaactgaa agtggacgcc
     1741 aagggggctc acggcgcgga tgacgccatc gatcttgccg atacgatcca gcatgacggt
     1801 gagtttttgt ccggcgagtg tttcggggac ttcggcgacc aggagggtgc cgtgcggcga
     1861 aaggtcggct gtgaaacccg tggctggggt gcctgcggcg gtgaaggaga agcctagtgc
     1921 gaccggctcg cgcagggact ggcgccgatt gccggcatcc gaggcgcgca ggatcactgc
     1981 aaggcggtga tgcaggtcgt gaatctcggt gctgacggat ttcgcgagcg caaggaactg
     2041 gcgggcggtc gtgtcggtgg tctgcgcctg ggtgtgcagt tcgtgggcat tactggcaac
     2101 ggagccgata tgttccgcag ccagcgtgac attgcgcatg atctcagcgg tggcgtcgcg
     2161 ctgttggtcg gtggcggccg agacctccgc ggcgatagcg tccacggcgt gcacctgatt
     2221 ggcgatctgg cccaccatgg tcgcggcgct gttggtggcg tcggcgattg aatgcgcctg
     2281 ggcgttgacg ttgccgatgg cgtcctcggt ctgggcggcg agctgcttca cttcgccggc
     2341 gaccacggcg aaacccttgc cggcatcgcc ggcgcgggcg gcctcgatgg tggcgttgag
     2401 ggcgagcagc ttggtctggc cggcgatcga gcggaccagc accacgacat cagcgatttt
     2461 gccgctggcc gatgagaggc ttttcacagc gtcgccagtt gccgcgacct gttgcacggc
     2521 ttgctgcgtc atgctggatg tgcggccgac gtgaccggtg atctcgcggc tcgatgcctc
     2581 gagctgctgg gtggcgctgg cgactgtctg gacgttttgg gcagtggtct cgaccgaggt
     2641 gctgacggcc tcggccatgg tgcggacctg ttcggcgacc tcgccgagtt gctcggccat
     2701 ctcggagagg cgctctgcct tggtggtgat ctcggcggcg ctcagcgcca tttcgcggtc
     2761 gagcacttcc gacatcgcca gcatctcgcg gcgcatgcgg ttggcctcac cggcctggat
     2821 gtaggtggag acggcgaggt ccatgtcgag gaaggcggca cgcagcactg cggcgatgtc
     2881 gttgggcagg tccttggcgc gagactttgc cgcgacggcg gcgatgaggt cggccagaat
     2941 gagggcgtag gcgccggtgt accagcgcgg ttcgagaccg atacggtcat gggcgtggcc
     3001 gatcgcaacg gcacgctgga agtaagcaag ggtgaacgtg ccctggaaca attcgtgcca
     3061 gtgctcgatc tgcgtcttct tgaggtgggc gatgcggtcg gggccgccga ggaggtgggc
     3121 caattccctc tgttggccca tgtaggcgta gaagcggtca gcaatgccag gcagggcggc
     3181 atagacgacc ggctgcatac ggcgcagtcg gtcgcacgtt gccgcatcga ttccgagga
//
LOCUS       DATGEM010000494         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_81389, whole genome shotgun sequence.
ACCESSION   DATGEM010000494 DATGEM010000000
VERSION     DATGEM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81389"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VKQ27_15365"
     CDS             complement(<1..258)
                     /locus_tag="VKQ27_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HLY90359.1"
                     /translation="MARSDLDARGRPVVVVTGTGVVTSLGAGKAENWRALTAGQSGIH
                     RISRFPIGGLRTNIAGTIDFVPVEEVSAPALSEQLAELVIDE"
     gene            complement(268..1452)
                     /locus_tag="VKQ27_15370"
     CDS             complement(268..1452)
                     /locus_tag="VKQ27_15370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005084.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HLY90360.1"
                     /translation="MASPRDAIITGIGLVSCLGEGVDAHWAALNNFRPVVDETTFAPF
                     PVHPMVTLELDKQIPKRGDQRQMEAWQRIGTYAAGLALDSAGVKGNAELLSRMDMIVA
                     AGGGERDYAVDTAILSALPAATDPGAYLNEHLLSDLRPTLFLAQLSNLLAGNISIVHG
                     VVGSSRTFMGEEASGADAIRIACARIAAGQGDLLLVGGSYNAQRPDVLLHYEMGGHLL
                     WKQPFAGVWERQAQGGGMVLGSLGCFLVIESREHAAKRNAAALAHIAAIATDRCRRRP
                     GEATANGEQQFADIRHHLAPEHAAVISGASGVAAATQEEAAFLQSLGLPVRGSATALG
                     HSLEPSFPANLALASMALSRRQLFAPLEASEQPMTAELRQVLVTSWGHWRGEAMAVVE
                     AA"
     gene            complement(1445..1915)
                     /locus_tag="VKQ27_15375"
     CDS             complement(1445..1915)
                     /locus_tag="VKQ27_15375"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HLY90361.1"
                     /translation="MRLEYFQMVDRIAALDPEARTVLAECSVPDASPVFEGHFPGHPL
                     LPGALMIETIAQTGGWLVLAMLRFQRMPFLAQVKEAKMRAFVVPGQTLQTEARLLHDG
                     SGYAVVAGSISSAGRKVADAEITYRVVPFPNETLRAQMLETARRVAVPEAFLNG"
     gene            complement(1923..2204)
                     /locus_tag="VKQ27_15380"
     CDS             complement(1923..2204)
                     /locus_tag="VKQ27_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLY90362.1"
                     /translation="MSSTFESVAGIIAETCDIPREQITPESHAINDLGIDSLDFLDVA
                     FAIDKAFGIKMPLEQWTQEVNEGKVSTDEYFVLGNLCANIDKLVAAKAA"
     gene            2194..>2375
                     /locus_tag="VKQ27_15385"
     CDS             2194..>2375
                     /locus_tag="VKQ27_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90363.1"
                     /translation="MDDIHQPLQRGRPLSPPPKCRKSGYIQWPARQTQPESVFSHQPG
                     IQLLLCPAASTYWPGA"
BASE COUNT          440 a          826 c          711 g          398 t
ORIGIN      
        1 ctcatcgatg accagctcgg caagttgctc cgacagagcg ggcgccgaca cctcttccac
       61 cggtacgaag tcgatggtgc ccgcgatatt ggtgcgcagc ccgccgatcg gaaacctgct
      121 gatgcgatgg ataccggact ggcccgccgt caacgcacgc cagttctccg ctttgcccgc
      181 accgagcgat gtgacgacac ccgtgcccgt cactaccacg acgggccggc cacgcgcatc
      241 gagatcggaa cgcgccatct ccgcctccta ggctgcttcc actaccgcca tcgcctcgcc
      301 gcgccagtgc ccccatgagg tcaccaatac ctgacgaagt tccgctgtca ttggctgttc
      361 gcttgcttca agcggcgcaa acaactgccg ccgcgacagc gccatagatg ccagcgcgag
      421 attggcagga aaggatggtt cgagcgaatg gccgagcgcg gtggcagacc cgcgcaccgg
      481 cagcccgaga ctttgcagga atgccgcttc ctcttgcgtg gcggctgcaa cgccggaggc
      541 accagaaatg accgcggcat gttccggcgc caggtgatgc cggatgtccg cgaactgctg
      601 ttcgccattg gctgtggcct cgcccggacg gcgccggcac cgatcagtgg caatcgcggc
      661 gatatgcgcc agtgcggcag catttcgctt tgcggcatgc tcgcggcttt cgatcaccag
      721 gaagcagccg agcgaaccca gcaccattcc gccaccctgc gcctggcgtt cccacacgcc
      781 ggcaaatggc tgcttccaca gcaggtggcc ccccatctca tagtgcagca gcacgtcggg
      841 ccgctgggca ttataagagc cgccgaccag caacagatca ccctgacctg ccgcgatccg
      901 cgcgcacgcg atgcgaatgg cgtcggcgcc cgatgcttcc tcccccatga atgtgcgcga
      961 tgatcccacc acgccatgca cgatcgaaat attgccggcc agcaggttcg atagttgcgc
     1021 cagaaacaac gttggccgca ggtcagacaa caggtgctca ttcagatacg cgccaggatc
     1081 ggttgcggca ggcagtgccg acaggatcgc cgtatccacc gcatagtccc gctcgccacc
     1141 gcccgccgcg acaatcatgt ccatgcgcga aagcagctcg gcgttaccct tcacgccagc
     1201 tgaatccagc gccaggcccg cagcgtaggt gccaatgcgc tgccacgcct ccatctggcg
     1261 ctggtcgcca cgctttggga tctgcttgtc cagctcgagc gtcaccatcg gatgcaccgg
     1321 aaacggcgcg aacgttgtct cgtccaccac tggacggaaa ttgttcagcg cggcccagtg
     1381 cgcgtcgacg ccttcgccga ggcatgagac cagaccgata ccggtaatga tcgcatcgcg
     1441 cgggctagcc attcaggaac gcctccggca cggcgacacg ccgtgcggtc tcgagcatct
     1501 gagcgcgcaa cgtctcgttg gggaagggga ccacgcgata ggtgatctcg gcgtccgcca
     1561 ccttccggcc cgccgaggaa atactgcccg ccacaaccgc atagccagac ccatcgtgca
     1621 gcaggcgcgc ctcggtctgc agagtctggc ccggcacaac gaaggcgcgc atctttgctt
     1681 ccttcacctg ggccaggaat ggcatccgct ggaaccgcag catcgccagc acgagccagc
     1741 cgccggtctg agcgatcgtc tcgatcatca gcgcgccagg cagcagcggg tggccgggga
     1801 agtggccctc gaacactgga ctggcgtcgg gcacggagca ttccgccaga actgtacgcg
     1861 cctccggatc gagcgctgcg atccggtcca ccatctggaa gtattccagc cgcatgctgg
     1921 ggtcaggcgg ccttggcggc caccagcttg tcgatgttgg cgcagaggtt gccgagcacg
     1981 aaatactcgt cggtcgaaac ctttccttcg ttgacctcct gagtccactg ctcgagcggc
     2041 atcttgatgc cgaacgcctt gtcgatggcg aacgccacgt ccaggaagtc caggctgtcg
     2101 atgccgaggt cgttgatggc atggctttcc ggcgtgatct gctcacgcgg aatgtcgcat
     2161 gtttcggcaa tgatcccggc gacggattcg aaggtggacg acattcatca gcccctgcaa
     2221 cgcgggcggc cactatcacc gccgccaaag tgccgcaaga gcggctacat ccagtggccg
     2281 gcccgccaga cccagcccga gtcggtcttc tcccaccagc cgggcatcca gttgctgcta
     2341 tgcccggcgg cctccacgta ctggccaggc gccca
//
LOCUS       DATGEM010000495         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1154461, whole genome shotgun sequence.
ACCESSION   DATGEM010000495 DATGEM010000000
VERSION     DATGEM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154461"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VKQ27_15390"
     CDS             <1..558
                     /locus_tag="VKQ27_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HLY90364.1"
                     /translation="AAGVGAVLTQHRTDPRLGPRGIELLRSGCSAQETLDALVASTSL
                     ARWRQLAVLDAAGNTAAYSGARTKPQMSEAPGQDVCAIGNILANAMVPAAMLRAFQSD
                     PSALLAERLLYALEEGLAAGGENGPVRSAHLLVVERESFPLVDLRVDWHDEPIAELRS
                     LWNRYAPQVNDYLIRALDPDDPTVL"
     gene            895..2262
                     /locus_tag="VKQ27_15395"
     CDS             895..2262
                     /locus_tag="VKQ27_15395"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HLY90365.1"
                     /translation="MWNPTDWRACPIAQAPAYPDADALDAAEAELRGSPPLIFAGEAR
                     ALKAQLARVADGHGFLLQGGDCAESFAEFHANALRDTVKVLLQMAVVLSYAGGVPVVK
                     VARMAGQFAKPRSQPTETTDGVTLPSYRGDIVNDIAFGATARTPDPARMLRAYNQSAI
                     TLNLLRAFTQGGFADLARVQQWNQAFVAASPQGERYREMSRRIEESLAFMAACGLTAA
                     NIPQLRSTDIYTSHDAMLLNYEEPLARQDSLTGDWYACSTHLPWIGERTRQADGAHVA
                     FLRGVANPLGLKCGPEMQPDDLLRVIDVLDPHNEPGRLTLITRMGADRIGSGLPPLVR
                     AVQHEGRRVVWSCDPMHGNTVSTATGRKTRPFQRILAELRTCIAVHRAESSHLGGIHI
                     EMTGKDVTECTGGAQQIDEATLADRYHTHCDPRLNGSQALELAFLVAEELRPQRAPVT
                     ALAAE"
     gene            complement(2266..2694)
                     /gene="msrB"
                     /locus_tag="VKQ27_15400"
     CDS             complement(2266..2694)
                     /gene="msrB"
                     /locus_tag="VKQ27_15400"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLY90366.1"
                     /translation="MRLFGSSHEAGTETFPVAHSEEKWRAKLSPAAYHVLRKHGTERA
                     GTSPLNAEKRHGAFVCAGCGQKLFASDTKFESGTGWPSFFQPVEGAVGTNEDRSFFMA
                     RTEVHCSRCGGHLGHVFDDGPRPTGQRYCMNGVAMDFKPD"
     gene            2763..3950
                     /locus_tag="VKQ27_15405"
     CDS             2763..3950
                     /locus_tag="VKQ27_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLY90367.1"
                     /translation="MAAFRDALQPVLPEDAGTAALAGRIWRPAVSGPSVVAVRDGELF
                     DVSSTFPTMRDLCESDAPADRLAAASGERMGSVDDVLANTPADARDRTKPWLLPPIDL
                     QAIKAAGVTFVLSMIERVIEERARGDMTAAVEIRAVVNRLVGDDLAKLKPGSPEAAHL
                     KQVLIEQGAWSQYLEVGIGPDAEIFTKAQPMAAVGSGMDAGIHPNSSWNNPEPEVVLA
                     AASTGRIVAATLGNDVNLRDVEGRSALLLGKAKDNNASCAIGPFLRFFDAAFSLDDIR
                     NTTVSLTVEGTDGFRLNGSSSIAKISRDPADLVGQMVNRHHPYPDGAVLFLGTMFAPV
                     EDRDAPGKGFTHKTGDVVTIAAPKLGRLVNRIRPTDQCEAWAFGVGALLRNLASRGLY
                     SGR"
     gene            complement(3971..4594)
                     /locus_tag="VKQ27_15410"
     CDS             complement(3971..4594)
                     /locus_tag="VKQ27_15410"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HLY90368.1"
                     /translation="MPQTASVLLTIALLWSATAVAPGPNFFLTTRTALLKSRATGVQT
                     ALGIACGSSIWGLCGFFGIHTVFTLAPWLYLALKVGGSIYLIAIGGRYLVNSFRPVTA
                     VPVMPQPTSTVAAVRLGMVTSLANPQSALSTASLFAATLPPHPSLWLGAGAITVMTAV
                     AAIWYGFVACVLTMRPAAAAFARLRRWIDRVAGLAFLGFGTRLALER"
     gene            complement(4594..>5153)
                     /locus_tag="VKQ27_15415"
     CDS             complement(4594..>5153)
                     /locus_tag="VKQ27_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90369.1"
                     /translation="VDQVSGGRFLFGIGIGWNAEEMEDHGTAFTTRAKLVRERVEAMK
                     LIWTKSKAEYNGELVKFPEMMTWPKPVQKPHPPVIVGGAFPQAARRAVRYGEGWIPLA
                     GRPGQYGDVFEVVPKFREMLKEAGRDEASCPVTLFNVQEDGDLLKRYRDLGAVRVSVS
                     LASEKADTILPILDRWAKLIQQVNG"
BASE COUNT          918 a         1721 c         1653 g          861 t
ORIGIN      
        1 gccgctggag tcggcgccgt gctgacccag catcgcaccg atccgcgatt gggaccacgc
       61 gggatcgagt tgctgcgttc tggctgctcg gcgcaggaga cgctggacgc gctggtcgcc
      121 tccacctctc tggcgcgctg gcgtcagctt gccgtgctgg atgcggccgg caacaccgcg
      181 gcatatagcg gcgcacgcac caagccgcag atgagcgagg cacccggtca ggatgtctgc
      241 gccatcggca acatcctggc aaacgcgatg gtgccggcgg cgatgctgcg cgccttccag
      301 tcggacccct cggcgttgct ggccgagcgg ctgttgtacg cccttgaaga aggattggcg
      361 gcaggcggcg agaacggccc ggtgcgcagc gcgcacctgc tggtggtgga acgcgagagc
      421 ttcccgctgg tggatctgcg cgtggactgg cacgacgagc caattgccga gctgcgctcg
      481 ctatggaacc gctacgcacc gcaggtgaat gactatttga tccgcgcgtt ggacccggac
      541 gatccaactg tcctttgatt gtctcttgcc atcattccgc cgatcggagt aagaccgcgc
      601 cgatggcacg gatagcacgt cccgcttcgc gattttggta ccgctggtat tacagcccgg
      661 cggcctagga tcgtgcacca gtagcaggaa cccaaagccg ccccgccaag gcggctttcg
      721 catttctgcg ccatcggaaa tcggattttc gccaaggcag gagcggcctc tcaaccgaag
      781 gaggctcgca tgcagagttc agatttcgat gtcgtcaccg gaccctcggt gccgcgcaag
      841 ccgatacccg cgccggtgcc gcagccggcg ccgcaacggc cacgatgacg cgtcatgtgg
      901 aacccaactg attggcgtgc ctgcccgatc gcgcaggctc ccgcctatcc cgacgctgac
      961 gcgctcgacg cagcggaagc ggaattacgc ggctcgccac ccctgatctt cgccggcgag
     1021 gctcgcgccc tgaaggcgca gctcgcccgc gtggccgacg gtcacggctt tctgctgcaa
     1081 ggcggcgact gcgccgaatc cttcgccgag ttccatgcca acgcgctgcg cgacaccgtg
     1141 aaggtactgc tgcagatggc ggtggtgctc agctacgcag gcggcgtgcc ggtggtgaag
     1201 gtggcgcgca tggcggggca gttcgccaag ccgcgctcgc agccgaccga gacgaccgat
     1261 ggcgtcacgt tgccgtccta tcgcggcgac atcgtcaacg acattgcgtt cggcgcaacg
     1321 gcacgcacgc ctgatcctgc gcggatgctg cgcgcgtaca atcagtcggc gatcacgttg
     1381 aacctgctgc gcgcgttcac acagggaggc ttcgccgatc tggcacgcgt gcagcagtgg
     1441 aaccaggcct tcgtcgcggc ctcgccacag ggcgagcgtt atcgcgagat gtcgcgccgc
     1501 atcgaggagt cgctggcgtt catggcggcg tgcggtttga ccgcggccaa catcccgcag
     1561 ctgcgttcca ccgacatata caccagtcat gatgcgatgc tgctcaacta cgaggaaccg
     1621 ctggcgcgtc aggacagcct gaccggcgat tggtatgcct gttcgacgca tctgccgtgg
     1681 atcggcgagc gcacgcgcca ggccgatggc gcgcacgtcg cgttcctgcg cggcgtcgcc
     1741 aatccgctcg gtttgaaatg cgggccggag atgcagcccg atgatctgct gcgggtgatc
     1801 gacgtgttgg atccgcacaa cgaaccgggc cgcctcacgc tgatcacgcg catgggcgcc
     1861 gaccgtatcg gcagcggcct tccaccgttg gttcgcgctg tacagcatga gggccggcgc
     1921 gtcgtgtggt cctgtgaccc gatgcacggc aataccgtca gcacggcaac cggtcgcaag
     1981 acgcggccgt tccaacgcat cctcgccgaa ctgcgcacct gcatagcggt gcatcgtgcg
     2041 gagagcagcc acctcggtgg catccacatc gagatgaccg gcaaggacgt caccgaatgc
     2101 actggcggcg cccagcagat cgacgaggcg accctggccg atcgctacca cacgcattgc
     2161 gatccgcggc tgaacggctc gcaggcgctc gaactcgcat tcctcgtagc tgaagaactg
     2221 aggccgcagc gcgcaccggt gacggcgctc gccgcggagt gacggtcagt ccggtttgaa
     2281 gtccatcgcc acaccgttca tgcaatagcg ctgaccggtg gggcgaggac cgtcatcgaa
     2341 cacatgcccc agatggccgc cgcaccggct gcaatgcacc tcggtgcgcg ccatgaagaa
     2401 gctgcggtcc tcgttggtgc cgacagcgcc ttccaccggc tggaagaagc ttggccatcc
     2461 ggtgccgctt tcgaatttcg tgtcagacgc aaacaacttc tgaccgcagc cggcgcagac
     2521 aaacgcgcca tgccgcttct ccgcattcag cgggctggtg ccggcgcgct cggtcccgtg
     2581 cttgcgcagc acatgatacg cggcggggga aagcttcgcg cgccatttct cttcgctgtg
     2641 ggccaccggg aatgtctccg ttcccgcttc gtgtgacgag ccgaacagcc gcatgggcct
     2701 gtctcctcgg ttcggggttg gcgatgttat aggatggaat ccaacgcata gggaggactc
     2761 caatggctgc attccgtgac gcgctgcaac cggtgctgcc tgaggatgcc ggcacggcgg
     2821 ctctggccgg ccggatctgg cgtccggccg tcagcggccc gtcggtcgta gcagtacgtg
     2881 acggcgaact gttcgatgtc tcgtccacgt tccccaccat gcgcgatcta tgcgaatccg
     2941 atgcgccggc agatcggctg gccgccgcat ctggcgagcg catgggcagc gtcgatgacg
     3001 tgctggccaa cacacctgcc gacgcgcgcg atcgcacgaa gccgtggctg ttgccgccga
     3061 tcgacctgca ggcgatcaag gcggcgggcg taacattcgt gctttccatg atcgaacgcg
     3121 tgatcgagga acgcgcgcgc ggcgatatga ccgccgccgt ggaaatccgc gcggtggtga
     3181 accggctggt tggcgacgac ctcgcgaagc tgaagcccgg ttcgccggaa gcggcgcatc
     3241 tgaagcaggt gctgatcgag cagggcgcct ggagccagta cctcgaagtt ggcatcggtc
     3301 ccgacgccga gatcttcacc aaggcccagc caatggccgc cgtcggcagc ggaatggatg
     3361 ccgggatcca tcccaactcg tcgtggaaca accccgagcc cgaggtggtg ctggccgccg
     3421 cctccacggg ccgtatcgtc gccgcaacgc tgggcaacga cgtgaacctg cgcgacgtgg
     3481 aaggccgctc ggcgctgctg ctgggaaagg ccaaggacaa caacgcgtcg tgcgcgatcg
     3541 ggccattcct gcgattcttc gacgcggctt tctcgctcga cgatatccgc aacacgacgg
     3601 tatcgctaac agttgaagga accgatggct tccgcctgaa cggttcgtca tcgatcgcga
     3661 agatcagccg cgatcccgcc gatctggtcg gacagatggt gaaccggcat cacccttacc
     3721 cggatggcgc cgtgctgttc ctcggcacca tgttcgcacc agtggaggac cgcgacgcgc
     3781 caggcaaagg cttcacacac aagaccggcg acgtggtgac gatagcagcg ccgaaactcg
     3841 ggcggctggt caaccggatc aggccgacgg atcaatgcga agcgtgggcg ttcggcgtgg
     3901 gcgcgctgct gcgcaacctc gccagccgcg gcctgtattc cggacgataa acgtcaggtc
     3961 cgcgcgcgct tcaccgttcc agcgcgagcc gcgtcccgaa cccaaggaac gccaggcccg
     4021 cgacgcggtc gatccagcgg cgcaaccgtg cgaacgccgc ggcagcaggg cgcattgtca
     4081 gcacgcaggc cacaaacccg taccagatgg ccgccaccgc cgtcatcacc gtgatcgcgc
     4141 cagccccaag ccacaacgat gggtgcggcg gcaatgtcgc ggcaaacagg ctcgcagtgg
     4201 acagcgcgct ttgcggattg gcgaggctcg ttaccatacc cagccgcacg gctgcgactg
     4261 tcgatgttgg ctgcggcatc actggaacgg cggtgacggg gcggaagctg ttcaccaggt
     4321 atcgcccgcc gatggcaatc agatagatgc tgccgccaac cttcagagcc aggtaaagcc
     4381 acggtgccag tgtgaaaacc gtgtggatgc cgaagaagcc gcacagaccc cagatgctgc
     4441 tgccgcaagc gatacccaac gcggtctgca ccccggtcgc gcggctcttc agcaacgctg
     4501 tgcgcgtggt cagaaagaag ttcgggcccg gggcaactgc ggtcgctgac cacagcaacg
     4561 ctatggtcag cagcaccgaa gctgtctgtg gcatcagccg ttgacttgct ggatcagctt
     4621 cgcccagcga tccagtatcg gcaggatggt atccgctttc tcggacgcca ggctcacgct
     4681 cacgcgcacc gcaccaagat cacgatagcg cttcagcagg tcaccatcct cctgcacgtt
     4741 gaacaacgtg acggggcatg atgcctcatc acgaccggct tccttcagca tctcgcggaa
     4801 cttcggcacg acctcgaaca catcgccata ctgaccaggc cggccggcaa gcggtatcca
     4861 cccttcgcca taccttaccg cgcggcgcgc cgcctgcggg aaggcgccgc cgacaatcac
     4921 cggcggatgc ggcttctgca cgggcttcgg ccacgtcatc atctcgggga atttgacgag
     4981 ttcgccgttg tactccgcct tcgacttcgt ccagataagc ttcattgcct cgacacgctc
     5041 gcgcaccagc ttggcacgcg tcgtgaaggc ggtgccgtgg tcctccattt cctccgcgtt
     5101 ccagccgatg ccgatgccga acaggaaccg tccgccggac acctggtcga ctg
//
LOCUS       DATGEM010000496         3507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1653687, whole genome shotgun sequence.
ACCESSION   DATGEM010000496 DATGEM010000000
VERSION     DATGEM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653687"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..928
                     /gene="gsiC"
                     /locus_tag="VKQ27_15420"
     CDS             8..928
                     /gene="gsiC"
                     /locus_tag="VKQ27_15420"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HLY90370.1"
                     /translation="MLGYFLRRLAGTIPVLIAVSILVFGFVRLLPGDPARLVAGPDAS
                     ESDVQVVRQDLGLDRPIYWQYAHYVGDIVQGNLGRSSRTRQPVSYEIGQRFMPTLWLT
                     LAAMGWAIVAGMGIGVLSGVRRGRWEDQGGMVVAVSGLSFPPFWLGLLLIDLFSVRLG
                     WFPTGGYESPASIVLPSFTLGLAVAAVLARFTRSAFVEVASEDYVRTARAKGLPERRV
                     IWTHTLRNALIPVITMTGLQFGFLLGGAVVVETVFAWPGLGRLLVDSISYRDYPVIQA
                     EILLFSLEFILINLAVDLLYAVVNPEIRLR"
     gene            925..1794
                     /locus_tag="VKQ27_15425"
     CDS             925..1794
                     /locus_tag="VKQ27_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY90371.1"
                     /translation="MSASTVVAARIDSPLAEFWRRFRRKHVALAAGFVLLLIILAAIA
                     APLIAPYDPSTPDYDNVLSPPTLAHLFGTDSYGRDILSRVIWGGRISLSVGFISVIVG
                     GVVGVALGLLSGFFGGWVDSLIMRGADVLFAFPGLLLALAVVAVLGPGISNVIWATAV
                     FGVPAFARLVRGDTLALKQAVYVQAARSIGVSRLKLVSVHILPGTMPGVIVNASLRLG
                     NNILTAASLSFLGLGAQPPSPEWGAMLADGRSYLGVADWLTLFPGLAIFVVVLAFNVL
                     GDGLRDALSQKLR"
     gene            complement(1850..2968)
                     /locus_tag="VKQ27_15430"
     CDS             complement(1850..2968)
                     /locus_tag="VKQ27_15430"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HLY90372.1"
                     /translation="MQWEDIMALTTVVRGRAYDWSHAIGRGAATGTGFNYVQTMCLGN
                     DGDLYTANRGNENNFGMRVNRIRLGSAGDEELVAEFFQHGEGDGKATWPFGVAVGQNG
                     HVYCSDEWTNTISVFDPNGKFQFKFGKTGSGDGELLRPAGIACEKNGNVIVVDSGNNR
                     LQVFSPEGKLAASCGRAGSGPGEFNQPWGVTLDHAGNIYVADWKNHRVQKLSPQGHHL
                     MSIGQYGKLPHPEDAFAVTYLGPFVSSLPTSNYPKAGLLNHPTDVAVDPDGDIYITDW
                     GNHRVCIFEADGTPLTNLYGDSQVMSKWAQQSVDANPDMMKAYRRVRNLEPLWRFCFP
                     TAVDFNPATDEIIVADSQRNRLQVYRKVRNYVDFQANL"
     gene            complement(3026..>3507)
                     /locus_tag="VKQ27_15435"
     CDS             complement(3026..>3507)
                     /locus_tag="VKQ27_15435"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLY90373.1"
                     /translation="TTIAQKLRGIAQVHLAGLGTRIFYCDHGSFDSHAGQAPLHAGLW
                     TAVTEGLEAFMADLREHNASDNVIVLMFSEFGRRVRDNGSGTDHGAAGATFVLGEKVK
                     GGHYGEYPSLDASKLVQGDLNPSMDFRSIYSTILDKWLGLNPAPIVNGTYEKPAFLH"
BASE COUNT          555 a         1120 c         1100 g          732 t
ORIGIN      
        1 cacgcaagtg ctgggctatt tcctgcgccg gcttgcggga acgataccgg tgctgatcgc
       61 cgtgtcgatc ctcgtgttcg ggttcgtccg gctgttgccg ggcgacccgg cgcggctcgt
      121 cgcaggaccc gacgcatccg aatccgatgt tcaggtggtg cgccaggatc tcggcctcga
      181 ccgcccgatc tactggcaat acgcgcacta cgtaggggac atcgttcagg gcaacctcgg
      241 ccgttccagc cgcacgcggc agcccgtttc ctacgaaatc ggccaacgtt tcatgccgac
      301 cctatggctg acgctggccg caatgggttg ggcgatcgtg gccggaatgg gcatcggcgt
      361 gctgtcgggg gtacgacgag gacgatggga ggatcagggc ggcatggtgg tcgccgtgtc
      421 tggcctgtcc tttccgccat tctggcttgg cctgctgctg atcgatctgt tctcggtgcg
      481 cctcggctgg ttcccgaccg gcggctacga gtcaccagct agtattgtgc tcccctcctt
      541 cacgctgggc ctggccgtcg cggcggttct cgcacggttc acccgttccg ccttcgttga
      601 agtcgcctca gaagactatg tgcgcacagc gcgcgccaag ggcctgcccg aacggcgagt
      661 gatctggacg cacacgctgc gcaacgcgct gatcccggtc atcaccatga ccggccttca
      721 gttcggcttc cttctcggcg gcgcggtggt ggtcgagacg gtgttcgcct ggccaggcct
      781 cggccgcctg ctggtcgact ctatcagtta ccgtgactac ccggtgatcc aagcggagat
      841 cctgctcttc tcgctggaat tcatcctgat caacctcgcg gtcgatctgc tgtacgccgt
      901 ggtcaacccg gagatccgcc tgcgatgagt gcatccacag tcgtagcggc acggatcgac
      961 agcccgctcg cggagttctg gcggcggttc cggcgcaagc acgtggcgct ggccgctggt
     1021 ttcgtcctgc tgctgatcat cctcgccgcg atcgccgcgc cactgattgc gccctacgat
     1081 ccctccacgc cggattacga caacgtgctg tcgccgccca cgctcgcgca cctgttcggc
     1141 accgacagct acggccgcga catcctgagc cgcgtgatct ggggcggccg catttcgctc
     1201 tcggttggct tcatttccgt catcgtgggc ggcgttgtcg gtgtggcgct cgggctgctc
     1261 agtggcttct tcggcggctg ggtcgatagc ctgatcatgc gcggcgcaga tgttctgttc
     1321 gccttcccgg gactgttgct ggcactcgcc gtggtcgcag tgctgggacc tggcatctcc
     1381 aacgttatct gggcgaccgc ggtgttcggc gttcctgcct tcgcgcgcct ggtccgcggc
     1441 gatacgctcg cgttgaagca ggccgtgtac gtacaggcgg cgcgcagtat cggcgtctcg
     1501 cgcctcaagc tggtgagcgt gcacatcctg ccaggaacga tgcccggtgt gatcgtaaac
     1561 gcatcgcttc ggctcgggaa caacatcctg accgccgcca gcctcagctt cctcgggctg
     1621 ggtgcgcagc cgccaagccc ggaatggggt gccatgctgg ccgatggtcg cagttatctc
     1681 ggcgtcgcgg actggctgac actgttcccc ggtctcgcga tctttgtggt cgtgctggcg
     1741 ttcaacgtgc tgggcgacgg gctgcgcgac gcactgagcc agaagctgcg ctgattggcg
     1801 cgttcagggg aacatggtgt tcgctcacca tgttccccgg gcgcggagtt cagagattcg
     1861 cttggaaatc gacgtagttg cgaaccttgc gatagacttg cagacggttg cgctggctat
     1921 ccgccacgat gatttcatcc gtcgccggat tgaagtccac cgccgtcggg aagcagaagc
     1981 gccacagcgg ttccaagttc ctgaccctgc gataggcctt catcatgtca gggttggcat
     2041 cgacgctctg ctgtgcccat ttcgacatga cctgagagtc accgtagagg ttggtcaacg
     2101 gcgtgccgtc ggcttcaaat atgcagaccc ggtgattgcc ccagtcggtg atgtagatgt
     2161 cgccgtccgg atccacagca acatcggtcg ggtggttgag caaaccagcc ttcggatagt
     2221 tcgacgtcgg cagactcgag acgaagggtc cgagataggt caccgcaaaa gcgtcttcag
     2281 gatgcggaag cttgccgtac tggccaatgc tcatcaggtg gtggccctgt ggcgacagct
     2341 tctgcacgcg gtggttcttc cagtctgcta catagatgtt gccggcgtgg tccagggtga
     2401 ccccccaagg ctggttgaac tcgcccggcc ccgaccctgc cctgccgcat gatgccgcca
     2461 gtttgccttc cggcgagaac acctgcagcc ggttgttgcc gctatcaacc acaatgacgt
     2521 tgccgttctt ctcgcaggcg atgccggccg gccgcagcag ttcaccatca ccgctgccgg
     2581 ttttaccgaa cttgaactgg aacttcccgt tgggatcgaa caccgaaatc gtgttggtcc
     2641 actcgtcgga gcagtaaacg tggccattct ggcccacggc gacgccaaac ggccaggtag
     2701 ccttgccgtc gccctcgccg tgttggaaga actcggcaac cagctcctca tcgccggcgc
     2761 tgccgagcct gatgcggttc acccgcatgc cgaagttgtt ctcattcccg cggttcgcgg
     2821 tgtagaggtc gccgtcgttg cccaggcaca tcgtctggac atagttgaac cctgtcccgg
     2881 tcgcggcgcc gcgcccgatg gcatggctcc agtcataagc cctgccccgc accactgtcg
     2941 taagcgccat gatgtcctcc cattgcatcg caaggttgtt cccctcccct ccgatgccgg
     3001 aagggagggg cagccatcag ccgcctcagt gcaggaaggc tggcttctcg taagtgccat
     3061 tcacgatcgg cgcggggttc aggccgagcc acttgtcgag gatcgtcgaa tagatgctgc
     3121 ggaagtccat gctcgggttg agatcgccct gcaccagttt ggacgcgtcc agcgacgggt
     3181 attcgccata gtggccgccc ttgaccttct cgccgaggac gaaggtcgcg ccagcagctc
     3241 catgatcagt accggagccg ttgtcgcgaa cgcggcgccc aaactccgag aacatcagca
     3301 cgatgacatt gtcagaagcg ttgtgttcgc gaaggtcggc catgaaggct tcgaggcctt
     3361 cggtcaccgc agtccacagg ccggcatgca acggagcctg gcctgcatgt gagtcgaagc
     3421 tgccatgatc acaatagaat atccgggtgc cgagaccggc gagatgcacc tgggcaatgc
     3481 cgcggagctt ttgcgcgatc gtggtgt
//
LOCUS       DATGEM010000497         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_109335, whole genome shotgun sequence.
ACCESSION   DATGEM010000497 DATGEM010000000
VERSION     DATGEM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109335"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VKQ27_15440"
     CDS             complement(<1..621)
                     /locus_tag="VKQ27_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY90374.1"
                     /translation="MALTIPALLAERIAAHGKEIVLRKKHRGIWQAVTWSQLGAHVAE
                     ISRGLAAIGLQPGDVACVLAETRPESAYVDLGIIEAGGVSGAINPATEADALGEALQQ
                     ANCRVLFVENEEQLDKVLLVRDGCPSLRHVVIFDMKGLRDFADPMCESLQQFIAHDGD
                     AASVTVTPDDPAVLLFPQMRVLTHGDALHMVTHACSLLRPVAGDERL"
     gene            complement(612..1385)
                     /locus_tag="VKQ27_15445"
     CDS             complement(612..1385)
                     /locus_tag="VKQ27_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90375.1"
                     /translation="MSAPLLDVTGIETFYGPIQAIRGVSLQVEQGSIVTVLGANGAGK
                     TTVLRTISGVLDPERGSVRFEGRDIANMAPDKVMRLGICHVPEGRETFPFLTVRENLM
                     MGAYTRHDKDAVHHDLELCYGYFPVLKERAHQNAGLLSGGEQQMLSISRALMGRPKLL
                     LLDEPSLGLSPLLVTQIFRIIRRINEEQGVAILLVEQNAHMALRTAQFGYVLEVGRIV
                     LADDCNSLMQTDAIKEFYLGQKEAGIRGQRRYKRKRLWH"
     gene            complement(1382..2146)
                     /locus_tag="VKQ27_15450"
     CDS             complement(1382..2146)
                     /locus_tag="VKQ27_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90376.1"
                     /translation="MILFRAEEIAVRFGGIRAVDAVSFDVEQGEVFSIIGPNGAGKTT
                     IFNLISRIYQPTSGRLFLEDREITDVPPYRIAGLGIARTFQNIELFPQATLLQNLLIG
                     RHCHSTVGVIPQLAFLPSVRREELKHREKVEEVIAFLGLERYRDTLIANLPYGVRKVV
                     ELGRALCIEPKLLLLDEPSSGLNVEETEGMGFWIEDIKKDLGITVIMVEHDMNLVRMV
                     SDRVMALNYGKVLALGSPDEVQNHPEVVRAYIGGEA"
     gene            complement(2143..3219)
                     /locus_tag="VKQ27_15455"
     CDS             complement(2143..3219)
                     /locus_tag="VKQ27_15455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY90377.1"
                     /translation="MRYVFRTDYRQDIALWRHSGDLFWYGLLCVALLVIPLVMGEFYV
                     GELGGVFIFAIAGVGMMLLAGYTGLISLGNAAFLGIGAYTNSILLAHGVPFLVSLPVA
                     GLFTALFGIAIGLPTLRMSGLYLAIATLAFGSIVGTVFQKWEAVTGGFDGFSVPTPYV
                     FGIPIETATGVYYVSLGVLAFVLWLSVNLLRSPLGRAMVAIRDSEISAQSMGIHLARY
                     KAFAFAISAGITGLAGALFAHYVHFLAPDSFDVLLSVQFVTIVFVGGLGSLHGAVLGA
                     IFVRLLPQFIAIIRDDLPWGIGRMPGLEPSLYGLVLVVVILFEPGGIWGRWLKMKHWF
                     QAYPLKRRTVFRRQKSYARSERLR"
BASE COUNT          659 a         1122 c          958 g          545 t
ORIGIN      
        1 caggcgctcg tcgccggcga ctggtcgcaa cagcgagcag gcgtgcgtga ccatatgcag
       61 cgcatcgcca tgcgtcagaa cgcgcatctg cgggaacaac agcacggcgg gatcatccgg
      121 cgtgaccgtg acacttgccg cgtcgccatc atgtgcgatg aactgttgca ggctctcgca
      181 catcggatcg gcgaagtcgc gcaaaccctt catgtcgaag atcacgacat ggcgcagcga
      241 tgggcagccg tcgcgcacca gcagcacctt gtcgagctgc tcctcgttct ccacgaacag
      301 cacgcggcag ttcgcctgct gcagcgcctc gccaagcgcg tccgcctccg ttgccggatt
      361 gatcgccccg ctcacgccgc ccgcttcgat gatcccgaga tcgacatagg ccgactccgg
      421 ccgcgtctcg gccagcacgc atgcgacatc gccaggctgc aagccgatag cagcgaggcc
      481 ccgacttatc tcggctacat gcgcaccgag ctgcgaccag gtcactgcct gccaaatgcc
      541 gcgatgtttc ttgcgcagca cgatctcctt gccgtgcgcg gcaattcgtt cagccaacag
      601 ggcgggaatt gtcagtgcca cagccgcttc ctcttatagc gtcgctgtcc gcggatcccg
      661 gcctctttct ggccgaggta gaactccttg atcgcgtccg tctgcatcag gctgttgcag
      721 tcgtctgcca gcacgatgcg gccgacctcc agcacgtagc cgaactgcgc ggtccgcagc
      781 gccatgtgcg cgttctgctc aaccagcagg atggcgacgc cctgctcttc gttgatgcgg
      841 cggatgatgc ggaagatctg cgtcaccagc agcggcgaca ggccgagcga tggttcgtcc
      901 agcaacagca gcttcggccg tcccatcagc gcgcggctga ttgacagcat ctgctgctca
      961 ccacccgaga gcaggccggc gttctgatgc gcgcgttcct tcaacacggg aaagtagccg
     1021 tagcaaagct ccaggtcgtg gtgcaccgcg tctttgtcat gacgcgtgta ggcacccatc
     1081 atcaggttct cgcgcacggt caggaacgga aaggtctcgc ggccttccgg aacgtggcag
     1141 atgcccaggc gcatcacctt gtccggcgcc atgttcgcaa tgtcgcgccc ctcgaagcgc
     1201 accgagccgc gttccggatc gagcacgccg gagatcgtgc gcagcactgt ggttttgccc
     1261 gcaccattcg cgcccagcac ggtgacgatg gagccttgct ccacctgcag gctgacgccg
     1321 cggatcgcct ggatcggtcc gtagaaagtc tcgatgccgg tgacgtcgag cagcggtgcg
     1381 ctcatgcctc gcccccgata taggcgcgca ccacctcggg atggttctgc acttcgtccg
     1441 gcgacccgag tgccagcacc ttgccgtagt tcagcgccat cacgcgatcc gacaccatcc
     1501 gcaccaggtt catgtcatgc tcgaccatga tcaccgtgat gccgaggtcc ttcttgatgt
     1561 cctcgatcca gaagcccatg ccttccgtct cttcgacatt caggccagag gacggttcgt
     1621 ccagcaacag cagcttcggc tcgatgcaaa gtgcgcggcc aagctccacc actttgcgca
     1681 caccgtaagg caggttggcg atcagcgtgt cgcggtagcg ctccagacca aggaaggcga
     1741 tcacctcctc gaccttctcg cgatgcttca gttcctcccg gcgcacgctc ggcaggaatg
     1801 cgagctgcgg gatcacgccg acggtcgaat ggcagtggcg gccgatcaac aggttttgca
     1861 ataatgtcgc ctgcggaaac agctcgatgt tctggaaggt gcgggcaatg ccaagaccgg
     1921 cgatgcgata aggcggaacg tcggtgatct cgcggtcttc caggaacaat cgtcccgatg
     1981 tcggctgata gatccggctg atcaggttga atatcgtggt cttgcctgcg ccgttcgggc
     2041 cgatgatgga gaatacctcg ccctgctcga catcgaagct caccgcgtcg acggcgcgga
     2101 tacccccgaa acgcaccgcg atctcttccg cccggaacag gatcatcgca gccgctccga
     2161 ccgtgcatac gacttctgcc gcctgaacac ggtccggcgt ttcagcggat acgcctggaa
     2221 ccagtgcttc atcttgagcc agcggcccca gataccgcca ggctcgaaca gaatcacgac
     2281 caccagcacc agcccataca atgaaggctc cagaccgggc atgcgcccga tcccccacgg
     2341 caggtcatcc cggatgatgg cgatgaactg cggcaataga cgcacgaaga tcgcgcccag
     2401 gaccgcgcca tgcagcgatc ccaggcctcc cacaaacacg atcgtgacga actgcaccga
     2461 cagcagcacg tcgaacgaat ccggcgccag gaagtggaca tagtgcgcga acagcgcacc
     2521 agccaatccg gtaatgccgg cactgatcgc gaaggcgaag gctttgtacc gcgccaggtg
     2581 gatgcccatg ctctgcgccg aaatctcgct gtcgcggatc gccaccatcg cgcggccgag
     2641 cggactgcgc agcaggttca cgctgagcca cagcacaaac gccaacacgc ccagcgatac
     2701 gtaataaacg ccggtcgccg tctcgatcgg aataccgaac acatacggcg tcggaacgga
     2761 aaagccgtcg aacccgcccg tcaccgcctc ccacttctgg aacacagtgc cgacgatgct
     2821 gccgaacgcc agtgtggcga tcgcgagata cagcccgctc atccgcagcg tcggcaggcc
     2881 gatggcgata ccaaacagcg cggtgaacag cccggccacc ggcagcgaaa ccaggaacgg
     2941 cacaccgtgc gcgagcagga tcgaattggt gtaggcgccg atacccagga acgctgcatt
     3001 gcccaggctg atcagaccgg tgtagccggc cagcagcatc atccccacgc cggcgatggc
     3061 gaagatgaac acgccgccga gttcgcccac atagaactcg cccatcacca gcgggatcac
     3121 cagcagcgca acgcagagca gcccgtacca gaacagatca ccgctgtgac gccacagcgc
     3181 gatgtcctgc cgatagtcgg tgcggaacac atagcgcatg gcgttacacc cgcttcccca
     3241 tgctttcggc gaacaggccc tgcggccgca ggatcagcgt cgcc
//
LOCUS       DATGEM010000498         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_585780, whole genome shotgun sequence.
ACCESSION   DATGEM010000498 DATGEM010000000
VERSION     DATGEM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585780"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VKQ27_15460"
     CDS             <1..622
                     /locus_tag="VKQ27_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLY90378.1"
                     /translation="ILAFMNSGQACAAGTRLLVPTSRLHAVKQAIRAAVSGFPVGDPL
                     DPKTVIGPMVSKKQYERVQSYIRKGVEEGAEVLVGGEGRPAGLEAGYFVKPTVFTNVS
                     NDMTIAREEIFGPVLSVITYDSEDEAIRIANDSKYGLHAAVLGADVERARRVASQLRA
                     GRVVINGMTDDPQAPWGGFNHSGIGREYGQYGIEAFLEPRAILEGQ"
     gene            627..1091
                     /locus_tag="VKQ27_15465"
     CDS             627..1091
                     /locus_tag="VKQ27_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY90379.1"
                     /translation="MSKHVTISPAEAADRLAIRELVEAYAHCADRRDAKGQMSLFTAD
                     THFVVYMNAKDPNPSMDLRGREALAPVFADLNKYDATTHFLGQSTLTALTSDRATGET
                     YCLAHHVTADGAKRRLMVASLRYLDTFSKMEGGWWFAERLLYVDWLEERALQ"
     gene            complement(1137..>2167)
                     /locus_tag="VKQ27_15470"
     CDS             complement(1137..>2167)
                     /locus_tag="VKQ27_15470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90380.1"
                     /translation="NVLGGSALFAVLFSMQLALGIVQAPTFPVSTGVFERWFPPRSWA
                     LVQGLQTMGLGFGGALAPPMVAALMTTIGWQVALLWTALPGIPLLLLWAWYGRNTPQE
                     HPSTSEKELALLGDARPAELSERTSRRGLGEILFDRRVLLLTFAYLSMNYVFYLLGNW
                     CFLYLVQAHHFSVRQSGILAIGPPIAAGIGAGLGGVIASRLHVRFGSRVGLRIVPTAS
                     LGAAAILLAAAVRANNAYVAITELTLCYGIIELTEGSFWAASMTIGRRDSMTVGGMMN
                     MGGSLGGVIGIPIVAYLSGRGLWNMAFLIGAATALASAIAWLFIDADERGSQPLGRAA
                     HVPGGVGA"
BASE COUNT          451 a          677 c          691 g          348 t
ORIGIN      
        1 cattctggcg ttcatgaaca gtggccaagc atgtgcggcc ggaacacgcc tgctcgtacc
       61 gacgagccgg ctgcacgccg tcaagcaggc catacgggcc gcagtgagcg gatttccggt
      121 cggcgaccct ctagacccca aaacagttat cgggccgatg gtttcgaaaa agcaatatga
      181 gcgcgtgcaa tcctacattc gcaaaggcgt tgaggaaggc gcagaagtgc tggtgggtgg
      241 agaaggccgc cctgcaggtc tcgaagccgg ctacttcgtg aagccgaccg tgtttaccaa
      301 tgtaagcaac gacatgacca tcgcacggga agagatattc ggccctgtgc tcagcgtcat
      361 cacgtacgac tcggaggacg aggcgatcag gattgcgaac gactccaagt atggcctcca
      421 tgccgccgtc ctgggagccg atgtcgagcg cgcacgtcgc gtagcctcgc aattgcgcgc
      481 tggccgtgtc gtgatcaacg gaatgacgga cgacccgcag gccccctggg gcggattcaa
      541 tcactcgggt atcgggcgcg aatatggcca atacgggatc gaggcattcc tcgagccgcg
      601 cgcgatcctg gaagggcagt gaaccgatga gcaagcatgt caccatctcc ccggcagaag
      661 ccgcggaccg gctcgcaata cgggaactcg tcgaggcgta tgcccattgc gccgaccgtc
      721 gcgacgcgaa gggacaaatg tctcttttta ccgccgacac acacttcgtg gtgtacatga
      781 acgccaagga tccgaacccg tcgatggatt tgcgcgggcg cgaggcgctc gcacccgtct
      841 tcgcggactt gaacaagtat gatgccacga cgcacttcct cggacagagc acgctcactg
      901 ccttgacgag tgatcgagcc accggagaaa cctactgctt ggcgcatcac gttacggcgg
      961 acggcgccaa gcggcgattg atggtcgcct cgctccgcta cctggacacc ttctcgaaaa
     1021 tggaaggcgg atggtggttc gcggaacgcc tgctctatgt cgactggctg gaggaacgcg
     1081 cactgcagtg actggaacgc cgctgcggcg ggcgagtgta ccgatcggcg cccggcctac
     1141 gcgccgaccc cgcctggaac gtgcgccgca cgccccagag gctgcgaccc ccgctcatca
     1201 gcgtcgatga agagccatgc gatagcgctc gccagtgcgg tcgccgcgcc gatcagaaac
     1261 gccatgttcc acaacccgcg gcccgacagg taggcgacga tcggaatacc aatgactccg
     1321 cccaggcttc ctcccatgtt catcattccg ccgacggtca tgctgtcgcg gcggccgatg
     1381 gtcatggagg ccgcccagaa ggatccctcc gtcaattcga tgattccgta gcacagggtg
     1441 agctcggtga tcgcgacgta cgcattgttg gcgcggaccg ctgctgcgag taggatcgcg
     1501 gcagcgccga ggctcgcggt cggaacgatg cgcaggccga cccggctgcc aaagcgcacg
     1561 tggagtctgc tggcaatgac gccgccgaga cctgcgccga tgccggctgc gatcggcgga
     1621 ccgatggcca gtattccgct ctggcggacc gaaaaatgat gtgcttgcac gagatacaga
     1681 aagcaccagt tgccgagcag gtagaacaca tagttcatcg acagataggc gaacgtgagg
     1741 aggagtaccc ggcggtcgaa caggatctcc cccagccctc tgcgtgatgt acgctcggag
     1801 agctcggcgg gtcgcgcatc gccgagtagt gccagctcct tctccgaggt cgagggatgt
     1861 tcctggggag tgttgcggcc ataccaggcc cacaacagga gcagcggtat gccgggcaaa
     1921 gccgtccaga gcagtgccac ctgccaacct attgtggtca tgagcgccgc gaccatcggc
     1981 ggcgccaacg cgccgccgaa tccgagaccc atggtctgga ggccctggac cagagcccag
     2041 ctacgcggcg ggaaccagcg ctcgaagaca cctgtggaga ccggaaaggt cggcgcctgc
     2101 acgataccga gagccagctg catcgagaag aggaccgcga atagtgccga gccgccgagc
     2161 acattcg
//
LOCUS       DATGEM010000499         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_152660, whole genome shotgun sequence.
ACCESSION   DATGEM010000499 DATGEM010000000
VERSION     DATGEM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152660"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VKQ27_15475"
     CDS             complement(<1..1006)
                     /locus_tag="VKQ27_15475"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90381.1"
                     /translation="MGRILSQDVAIQRKRLGRELGTIRKDWGGRLPIALCYPNSYAVG
                     MCNLGFQSVYNLFNSAKGFVCERVFAEPVLIGSRTAGRTDWTGDDNYRIQSRLEAIGE
                     PLSVESQRPLTDFAVVAFSLSFELDYFNIGDVLRRAGIAPLAQNRDETDPIVIAGGPA
                     VSGNPEPVAPMLDAIVIGEVEPVMDGLQDVFLGGGSRGEQIDKLARLPGVYVPAQYKI
                     GYAPDGTIARVDPSSSDLALPVARLNARNVNDFQTMSAVLSPDIELGDMFLLEMTRGC
                     ARGCRFCLAGYTSLPVRHRHVDHLMAGVEHGLKLRKRIGLISAATSDHPQLEQLLARM
                     L"
     gene            complement(1119..2348)
                     /gene="ftsZ"
                     /locus_tag="VKQ27_15480"
     CDS             complement(1119..2348)
                     /gene="ftsZ"
                     /locus_tag="VKQ27_15480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLY90382.1"
                     /translation="MNGYASLEGAARIVVLGVGGGGSNAVNRMIQAGVRGVEFVAINT
                     DTQALARSEAPTRIHIGEKLTRGLGAGGNPNVGEKAAEESGEQIADLVRDADMVFIAA
                     GMGGGTGTGAAPVIAQISKDLGALTVGVVTKPFTFEGRQRSKNADEGIAQLRDRVDTL
                     ITIPNDRLLEVIDRKTTLEQAFMEVDDVLRQGIQGISELITEPGLINLDFADVKSIMS
                     DSGGALMAIGRGTGETRAQDAARMAISSPLLDISMEGAKGVLLNITGGIDLALSEINE
                     AADVVAQAADPEANIIFGAVIDSKLENEIKITVIATGFDVSQRAGRPVPQFRRTRPSY
                     GGGSGEPPAVTEPPAAGAAGRDRALPVEDRDRDRPPEREQGPIRVGSIGSSLPEPVFN
                     EGDDDLDIPPFLRGRRR"
BASE COUNT          439 a          808 c          762 g          458 t
ORIGIN      
        1 ccagcatccg cgcgagcaac tgttcgagct gcggatggtc ggaggtcgcc gccgaaatca
       61 ggccgatgcg cttgcgcagc ttcagcccgt gctcgacacc cgccatgagg tggtccacgt
      121 ggcggtgacg cacgggcagc gacgtatagc cggccaggca gaagcggcag ccgcgggcgc
      181 agccgcgggt catctcgagc aggaacatgt cgcccagttc aatatccggg ctcaagacgg
      241 ccgacatcgt ctgaaagtcg ttgacgttgc gggcgttgag tcgcgccact ggcagcgcca
      301 gatcgctgct cgacggatcg acccgcgcga tcgtgccgtc tggggcatag ccgatcttgt
      361 actgcgccgg gacgtacacc ccaggcaacc tggccagctt gtcgatctgc tcgccgcggc
      421 taccgccacc gagaaaaacg tcctgcagcc cgtccatgac cggctcgacc tcgccaatca
      481 cgatcgcgtc gagcatcggg gcaacgggct cgggattgcc cgagacggca ggtccgccag
      541 cgatgacgat gggatccgtc tcgtcgcggt tttgcgccag cggcgcgatc ccagctcgac
      601 gtaagacgtc gccgatgttg aagtaatcca gctcgaacga caggctgaag gcgaccaccg
      661 cgaagtccgt cagcggcctt tgcgactcga ccgacagcgg ctcgccaatc gcttcgaggc
      721 ggctttgaat gcggtagttg tcgtcgccgg tccaatccgt tcggccagcc gtccgactgc
      781 caatcaggac cggctcggca aatacgcgtt cgcagacaaa ccctttggcg gagttgaaca
      841 ggttgtagac actctggaag cccaggttgc acatgcccac cgcgtacgaa ttcgggtagc
      901 acaaagcgat cggcagccgg ccgccccaat ccttacggat ggtcccaagc tcgcgcccga
      961 gccgcttacg ctggatcgcg acgtcttgag aaagaatgcg tcccataaag gagtcagaag
     1021 tcagacatca gaagtcagac gggacagtct aacggctgtc agccgcctca ggaggtttcc
     1081 gctgacaacg tctgatttct gacttctgac ttctgacttc agcgccgacg cccgcgcagg
     1141 aacggcggaa tatccagatc gtcgtcgcct tcgttgaaga cgggttcggg caggctcgag
     1201 ccgatcgagc cgacgcggat cggaccctgc tcgcgttcgg gcggacggtc acggtcgcga
     1261 tcttcaaccg ggagcgctcg gtcgcgcccg gccgcgccag ccgctggcgg ttcggtgacc
     1321 gcgggcggct caccactgcc gccgccatag cttggccgcg tgcgccgaaa ttgggggacg
     1381 ggccggcccg cccgctggct gacgtcgaac ccggtggcga tgaccgtgat cttgatctcg
     1441 ttttcgagtt tggaatcgat gaccgcgccg aaaataatgt tggcttcggg atcggccgcc
     1501 tgagcgacaa catcggccgc ttcattgatc tccgaaagcg cgaggtcaat cccgccggtg
     1561 atattgagca gaacgccctt ggcgccttcc atgctgatat cgagcagcgg gctggagatc
     1621 gccatgcgcg cggcgtcctg cgcacgcgtc tcgcctgtgc cgcgcccgat cgccatcagc
     1681 gcaccgcccg aatcggacat gatcgatttg acgtcggcaa aatcgagatt gatcagccct
     1741 ggttcggtga tcagctcgct gatgccctgg ataccctggc gcaagacgtc gtcgacttcc
     1801 atgaatgcct gttcaagggt ggttttacga tcgatcacct cgagcagccg gtcatttgga
     1861 atcgtgatca gcgtgtcaac ccgatcgcgc aattgggcga tgccttcgtc ggcattcttg
     1921 gagcgctgtc gcccttcgaa cgtgaacggc ttggtgacca ctcccaccgt gagcgccccg
     1981 aggtctttcg agatctgggc gattacgggg gcggcgcccg tgcccgtccc gccgcccatg
     2041 cccgcggcaa tgaaaaccat gtccgcatcg cggaccaggt cggcgatctg ttcgccgctc
     2101 tcttcggccg ccttttctcc gacgttcggg tttccgcccg caccgaggcc acgggtcagc
     2161 ttttcgccga tgtgaatgcg cgtgggcgcc tctgaacggg cgagcgcctg cgtgtcggta
     2221 ttgatggcga cgaattccac gccgcgcacc ccggcctgaa tcatccgatt gacagcattg
     2281 ctgccgccgc ccccgacgcc caggacgaca atgcgtgcgg cgccttcgag gctggcgtat
     2341 ccgttcattg tcaataccgt tccccccgca ttctaagctt tcatggaagg aaatttttca
     2401 gccagttcgt taccgcgctg ccgatgcccg acagcggcaa tccaccccgt gcagccgccg
     2461 gctcgaa
//
LOCUS       DATGEM010000500         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1179675, whole genome shotgun sequence.
ACCESSION   DATGEM010000500 DATGEM010000000
VERSION     DATGEM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179675"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..915)
                     /locus_tag="VKQ27_15485"
     CDS             complement(94..915)
                     /locus_tag="VKQ27_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY90383.1"
                     /translation="MADPVVLYQTDEKVGIITLNRPDKLNAISRELQHALTETFARAD
                     ADPTTTVVLLRAEGRSFSAGYDIGAKSPSDADDWRSDPTKAHAHLQPQLEFEMAPWLM
                     TKPVIASVQGHVLGGGCELVMLCDLTIAADNATFGEPEVRFSSVGPAIVMPMIIGYKK
                     ARELLYFGDQIDARTALDLGMINRIVPLAELRDASLAYAKRLSLISPEALYATKRAVN
                     RVADASGFRTALYAGLDVVGPLYATNTEYGARFREIAQTEGVPAAVRWRSAQFAK"
     gene            1088..1945
                     /locus_tag="VKQ27_15490"
     CDS             1088..1945
                     /locus_tag="VKQ27_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90384.1"
                     /translation="MGREGKWKTRAKHVGGSEGQDHALDLEAGLAEVEQQAEMQAGGI
                     ETIQALRTMNVVDCLGGFQLDEDDTIDEQIDGIFADHDPIVANDHGMLLRDGLLRSLP
                     ANSGKPSMTLAPIRGAPPGAPIPDFPVDEPKAKAAEPSAATVPDDQPEEPPFRQPYTP
                     LEVRGNGIILTFRVIAELPAISRASMTIQIAAQSAGASSASPLGFLRMSAAHARAFLA
                     GLRNSCSPIVATGDEDGTVQLEYEITETQPVMLVRQLAERDTTHRCIIDDEFDLRTMA
                     DELLADLGA"
     gene            complement(2005..>2647)
                     /locus_tag="VKQ27_15495"
     CDS             complement(2005..>2647)
                     /locus_tag="VKQ27_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY90385.1"
                     /translation="STVLFAVTDPSAGRKGISCFVVDKATPGYTVARREEKLGQKASE
                     TCALTFQDMEVPEDQRIGAEGEGYKIALSTLESGRIGIAAQSVGMARAALDYAVGYAK
                     ERQAFGGRILDFQAVGFRLADAKTRLEAARQLTLHAARLKTEGRPSLEAACMAKLFAS
                     EVAEAVCTAAIQTLGGYGYLADYPVERIYRDVRVCQIYEGTSDVQKLILQRML"
BASE COUNT          462 a          785 c          909 g          491 t
ORIGIN      
        1 gcgaggcgac ggacgacgtg tcgggcacgg cgttgaccat gccggggttt tagcaagacg
       61 cggaaaacga ggcgaggaaa atattccgat ggcctatttc gcaaactgag ccgagcgcca
      121 gcggaccgcg gcgggtacgc cttcggtctg cgctatctcg cggaaccgcg cgccgtattc
      181 ggtgttggtg gcgtagagcg ggccgacgac atcgagcccg gcgtagagcg cggtgcggaa
      241 gcctgaggcg tcggctactc ggttcacggc gcgcttcgtt gcatacagcg cttcgggcga
      301 gatcagcgac aggcgcttgg cgtaggcgag gcttgcgtcg cgcagttcgg cgagcgggac
      361 gatgcggttg atcatgccga ggtcgagcgc ggtgcgggcg tcgatctggt caccgaaata
      421 gagcagctcg cgcgccttct tgtagccgat gatcatcggc atgacgatgg cggggcccac
      481 cgaggagaag cggacctcgg gttcgccgaa ggtggcgttg tcggcggcga tggtcaggtc
      541 gcacagcatg acgagttcgc agccgccacc gagcacgtgg ccttgcacgg aggcgatcac
      601 cggcttggtc atcagccagg gcgccatctc gaattccagc tgcggctgga ggtgggcgtg
      661 cgctttggtg ggatcgctgc gccagtcatc ggcgtcggat gggctcttgg cgccgatgtc
      721 gtagccggcg gagaagctgc ggccttcggc gcggaggagc acaaccgtgg tggtggggtc
      781 ggcgtcggcg cgcgcgaagg tttcggtgag cgcgtgctgg agctcgcggc tgatcgcgtt
      841 gagtttgtcg gggcggttca gggtgatgat gccgactttt tcgtcggtct ggtacaggac
      901 tactgggtcg gccatggtgt gtgctcccac tggctgcgtt gatgcggcgt atgtgatgat
      961 cgctttaggg gcatgccgct gtgacggcaa acatggcggg cattgggcga gacactggag
     1021 cggctcgctt tggcggtgca acgtgcgtgt gcctcgtttc gggggctcac ccgtggggcc
     1081 ggtcaacttg ggccgcgaag gaaaatggaa aacacgcgca aaacacgttg gtggctcaga
     1141 gggccaggac catgcgcttg atctcgaggc ggggcttgcc gaagttgagc agcaggcaga
     1201 gatgcaggcc ggtggcattg agacaattca ggcattgcgc acgatgaatg tcgtcgattg
     1261 ccttggcggc tttcaactcg acgaggacga tacgatcgac gagcagatcg acggtatatt
     1321 cgccgaccac gatcccatcg tagcgaacga tcatgggatg ttgctgcgag acggcttgtt
     1381 gcgtagcctg ccagcaaata gcgggaaacc ctcgatgacc ctcgcgccga tacgaggcgc
     1441 cccgcccggc gcaccgatac cggattttcc ggtcgatgaa ccgaaagcaa aggcggcgga
     1501 accgagcgcg gcgacggtcc ccgatgatca accggaagaa ccaccattca gacagccgta
     1561 tacgccactc gaagttcgtg gcaacggcat catactgacg ttcagggtca tcgctgaact
     1621 gccggccatc tcgcgggcga gcatgaccat ccagatagct gctcagtcgg caggggcatc
     1681 atccgcaagc ccacttggct tccttcggat gtcagcggct cacgcgaggg cgttccttgc
     1741 tggtctacgg aacagctgct ctcccatcgt cgcaacggga gacgaggatg gtacggtgca
     1801 actggaatac gaaatcacag agactcagcc agtcatgctt gtccgccagt tggccgagag
     1861 ggacaccacg caccgctgca tcatcgatga tgagttcgac ctgaggacca tggctgacga
     1921 actccttgct gatctgggcg cctagctctg accataaatc cgcctatggc ttgccgaggc
     1981 gtcacgatcc ggtgctgggt gcattcagag catccgctgc aatatcagct tctgcacatc
     2041 tgacgtgcct tcgtagatct ggcacacgcg cacatcgcgg tagatacgct cgaccggata
     2101 gtccgcgaga tagccgtagc cgcccagcgt ctggatcgcc gccgtgcaca ccgcctcggc
     2161 cacttcgctc gcgaacagct tcgccatgca ggcggcttcc agcgatggcc gcccctcggt
     2221 cttcaggcgc gcggcgtgca gcgtgagctg gcgcgcggcc tccagcctgg tcttggcatc
     2281 ggccaggcgg aatcctactg cctggaagtc gagaatccgg ccgccgaacg cctggcgttc
     2341 cttcgcgtaa cccaccgcgt agtccagcgc ggcgcgcgcc atgccgacgc tctgcgcggc
     2401 gatgccgatg cggccgctct ccagcgtgga gagcgcgatc ttgtagcctt cgccttcggc
     2461 gccgatacgc tggtcctcgg gtacttccat gtcctggaag gtgagcgcgc aggtctcgct
     2521 ggccttctgg ccgagctttt cctcgcggcg cgcgaccgtg tagccgggcg tggctttgtc
     2581 gaccacgaag caggagatgc ccttgcggcc ggcgctgggg tcggtcaccg cgaacagcac
     2641 cgtgctg
//
LOCUS       DATGEM010000501         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1353450, whole genome shotgun sequence.
ACCESSION   DATGEM010000501 DATGEM010000000
VERSION     DATGEM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353450"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VKQ27_15500"
     CDS             <1..891
                     /locus_tag="VKQ27_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="catalyzes the formation of
                     tetrahydropteroyl-L-glutamate and methionine from
                     L-homocysteine and
                     5-methyltetrahydropteroyltri-L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HLY90386.1"
                     /translation="VAEQEKAGLDVITDGEQRRQHYIWGFFHGLDGIDTANLGLRPQR
                     AQRYRKEIVAARLIGEPTYRGPIFVDALRATKAMSDLPIKVTLPGPMTIVDSIIDDVG
                     GRSEADLAMRFAELLNIEARTLSDAGADVIQFDEPCFNVYVDKVRDWGMTALERAAQG
                     VTATKAIHICYGYGTAEVLRWKNSNTDWSHYEATLPLLAKSTIDQVSVETGASGVDIA
                     VVEALRGKDVLLGVVDVGTDEVETPEVVAERLHAALRYVDPQHLYACTDCGMMPRSRA
                     AARGKLHALAAGAALVNAMR"
     gene            920..2236
                     /locus_tag="VKQ27_15505"
     CDS             920..2236
                     /locus_tag="VKQ27_15505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90387.1"
                     /translation="MANEATIPFDAAPADERQRLHAIRLTATASLIGTTIEWYDFILY
                     TSLSGLIFNKIFFPANNPTVSLVLAYATFAVGFITRPIGGAFFGHFGDRIGRKPLLVL
                     TLTMMGAVTFLIGLVPSYAAIGIASPLIVVVLRLTQGFAMGGEWGGAVLMAFEYAPSE
                     RRGFYASFPQIGFAIGLCLSTGVITLLSSLLSDAAFLSWGWRCAFLVSIVLLGVGLFV
                     RLRLFETPEFERIRGSYHVTKLPIGTVARDYGGNVLLGWLARMGEGGIFTVYTLYMYS
                     YLISVIHLPRTLVLSSVTAAALVLIFTVPLASAWSDRVGRRRLFGIATLVNGIAAFPL
                     LWMLQSGIPELAAIGIVLSIGILWAPVYGPEAALFCELFATPVRYTGVSLVYQFGAII
                     FLAPVPILATLLVAWNGNQPWYLATYLLIACIISTIATGLMRRTYT"
     gene            complement(2240..3319)
                     /locus_tag="VKQ27_15510"
     CDS             complement(2240..3319)
                     /locus_tag="VKQ27_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY90388.1"
                     /translation="MKIATVDTGHYRIELPVVLSDSTHGTITHFELITVRLRDADGAE
                     GVGYTYTVGSGGGAVHALIDHDLRSALLGAEAEDIQALWRTMWWRLHFGGRGGSVSLA
                     VSACDIALWDLKAKRFNTPLWRLLGGFDPAVPCYAGGIDLDFPLDALLRQTDDNLGRG
                     FRAIKMKVGRPRLSEDAERVRAMRSHLGDDFPLMADANMRWTVDEAIRAARAFRDSNL
                     VWLEEPTIPDDIAGHARIVRDGGVPIATGENLHTLHEFKAMIEAGGVTFPEPDVTNCG
                     GITVFMKVAHLAEAYNLPLTSHGVHDLTVHLLAAAPNRSYLEVHGFGLDRYIDEALVI
                     RDGNAIAPERPGHGIVFDWAALERL"
     gene            complement(3316..>3982)
                     /gene="serS"
                     /locus_tag="VKQ27_15515"
     CDS             complement(3316..>3982)
                     /gene="serS"
                     /locus_tag="VKQ27_15515"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLY90389.1"
                     /translation="GQLPKFAEDNFRTTDGRWLIPTAEVPLTNTVAGDIVAEKVLPIR
                     LTALTDCYRSEAGAAGRDTRGMLRQHQFRKVEMVSITHPDQSADEHERMTRCAEKVLT
                     LLEVPFRRVVLSSGDTGFSAVKTHDLEVWLPGQQAWREISSCSNCRDFQARRMNARFR
                     SADGKTVAYVHTLNGSGIAVGRALIGVMENHQQADGSILIPEVLWPYMGGNSGSVQRI
                     APA"
BASE COUNT          736 a         1298 c         1200 g          748 t
ORIGIN      
        1 gtggccgagc aggagaaggc cggcctcgat gtcatcaccg acggcgagca gcgccggcag
       61 cactacatct ggggcttctt ccacgggctt gacggtatcg acaccgctaa cctcggcctg
      121 aggccacagc gcgcgcaacg ctaccgcaag gagatcgtcg ccgcgcgcct cattggcgaa
      181 cccacctatc gcggccccat cttcgtcgat gcactgcgcg ccaccaaagc gatgtcggac
      241 ctcccgatca aggtcaccct cccagggcca atgacgatcg ttgacagcat catcgatgac
      301 gtcggtggcc gcagcgaagc cgacttggca atgcgcttcg cggaactcct caacatcgag
      361 gcccgtacat tgtcggatgc cggcgcagac gtgatccagt tcgatgaacc atgtttcaat
      421 gtctacgtgg acaaggtgcg ggactggggc atgaccgcgc tggaacgcgc cgcccaaggt
      481 gtcaccgcca cgaaagcgat ccacatctgt tatggctacg gcactgccga agtgctgcgc
      541 tggaagaata gcaataccga ctggagtcac tacgaagcca ccctgccgct gctggcaaaa
      601 tccacgatcg accaggtctc ggtggaaacc ggggcaagcg gcgtcgatat cgctgtcgtt
      661 gaggcgttgc ggggcaagga tgtgctgctc ggtgtcgttg acgtcggcac cgacgaagta
      721 gagacgccag aggtcgtggc ggaacggctg catgccgcgc tgcgctacgt cgatccgcaa
      781 cacctctacg catgcaccga ctgtgggatg atgcctcggt cacgcgcggc ggcgcggggc
      841 aagctgcacg cattggcggc cggcgccgca ctggtcaacg caatgcgcta gccgcacaca
      901 aactcagggg gaaacgataa tggccaacga agccacaatt ccattcgacg ctgcgccggc
      961 cgacgaacgt caacgcctcc atgcgatcag gctgaccgct accgccagcc tgattggcac
     1021 gacgatcgag tggtacgact tcatcctcta cacctcgctg tcgggcctga tcttcaacaa
     1081 gatcttcttc cccgccaata acccgacagt atcgctcgtg ctggcctacg cgacgttcgc
     1141 cgtcggcttc atcacccgtc ccatcggagg cgccttcttc ggccatttcg gcgaccgcat
     1201 cggccgaaag ccattgctgg tgctgacgct cacaatgatg ggcgcggtga ctttcctcat
     1261 cggcctggtg ccaagctatg ccgcgatcgg catcgcctca ccgctgatcg tggtcgtact
     1321 gcgattgacc cagggcttcg ccatgggtgg cgaatggggc ggtgcggtgc tgatggcctt
     1381 cgaatacgcg ccatccgaac gacgtggctt ctacgccagc ttcccgcaga tcggctttgc
     1441 catcggcctg tgtctcagca cgggcgtgat cacattgctg tcctcgctgc tgtcggacgc
     1501 ggcgttcctt tcgtggggct ggcgatgcgc cttcctggtc agcatcgtgc tgctcggtgt
     1561 gggattgttc gttcgcctcc gtctgttcga gacgcccgag ttcgagcgca tccgcggcag
     1621 ctaccacgtc acgaaactac ccattggcac ggtggcgcgg gactacggcg gaaacgtcct
     1681 gctgggatgg ctggcgagga tgggcgaagg aggcatcttc accgtctaca ccctgtatat
     1741 gtactcatat ctcatcagcg tcatccatct gccgcgcacg ctggtactga gttcggtcac
     1801 cgccgcggcg ctggtactga tcttcaccgt tcccctggcc tccgcctggt cagaccgggt
     1861 cgggcggcgc aggctgttcg gcatcgccac gctggtgaac ggcatcgcag ccttcccgct
     1921 gttgtggatg ctgcaatccg gcattcccga gcttgcggcg atcggcatcg tgctatccat
     1981 cggcatcctg tgggcgcctg tctatgggcc ggaagccgcg ctgttctgtg agctgttcgc
     2041 gacgcccgtg cgctataccg gagtctcgtt ggtctaccag ttcggcgcga tcatcttcct
     2101 ggcgccggtg ccgatactcg caacattgct ggtggcgtgg aacggcaatc aaccgtggta
     2161 tctcgccacg tatctgctga tcgcatgcat catcagcacc atcgcgaccg gcctgatgcg
     2221 tcgaacctac acctgacggt tacagccgct ccagcgctgc ccagtcgaag actatgccgt
     2281 gtccgggacg ctccggtgcg atcgcattgc cgtcacggat caccagagct tcgtcgatat
     2341 agcgatcgag cccgaagccg tgcacttcca gataggaccg gttcggcgcg gcagccagca
     2401 ggtgcacggt cagatcgtgg acgccatgcg aagtgagcgg caggttgtac gcctcggcca
     2461 gatgcgcgac cttcatgaat accgtgatgc cgccacagtt ggtgacgtcg ggttcgggga
     2521 aggtcacacc gccggcctcg atcatcgcct tgaactcatg cagcgtatgc aggttctcgc
     2581 cggtcgcgat cggcacgccg ccatcacgca cgattcgcgc gtgaccggcg atgtcgtccg
     2641 ggattgtcgg ctcctccagc cagaccagat tgctgtcccg gaaagcgcgc gccgcgcgga
     2701 tcgcctcatc gactgtccag cgcatattgg catccgccat cagcgggaag tcgtccccca
     2761 ggtgggaacg catggcacgc acacgttccg cgtcctcgga aagccgtgga cgcccgactt
     2821 tcatcttgat cgcgcggaac cctctgccga ggttgtcatc agtctggcgc agcagcgcat
     2881 cgagcggaaa atccagatcg ataccgccgg cgtaacaggg cactgccgga tcgaaaccac
     2941 ccagcaaccg ccatagcgga gtattgaagc gcttcgcctt caggtcccac aatgcgatgt
     3001 cacaggctga aacggcaaga ctcaccgagc cgccgcgccc accgaaatgc agccgccacc
     3061 acatcgtccg ccacagcgcc tggatgtcct ctgcttctgc gccaagcagc gcggagcgca
     3121 agtcatgatc gatcagcgca tgcaccgcgc cgccacccga gccaacagtg taggtatagc
     3181 cgacgccttc cgcgccatcg gcatcgcgca agcggacggt gatcagctcg aagtgggtga
     3241 tcgtgccgtg agtggaatcc gacagcacga ccggcagttc gatccgatag tggcccgtat
     3301 ctacagtggc aatcttcatg ccggcgcaat ccgctgcacg ctgccactgt tgccgcccat
     3361 atagggccac agcacttcgg gaatcagaat cgagccgtcc gcctgctggt ggttttccat
     3421 gacgccgatc agcgcgcggc caacagcaat gccagagcca ttcagcgtat ggacataggc
     3481 gaccgtcttg ccgtcggcgg aacggaaccg cgcattcatc cgcctcgcct ggaagtcccg
     3541 gcagttggag caggaactga tctcgcgcca agcttgctgg ccaggtagcc acacttccag
     3601 gtcgtgggtc ttgacggcgc tgaaccccgt gtcgcccgac gacagaacaa cgcggcggaa
     3661 cggcacttcc agcagtgtga gtaccttctc cgcgcagcgc gtcatgcgtt catgctcatc
     3721 ggcgctctgg tcgggatggg tgatcgagac catctcgacc ttgcggaact ggtgttggcg
     3781 caacatgccg cgcgtatcac gaccagccgc gccagcctcg ctgcgatagc agtctgtcag
     3841 agcagttaag cgtatcggca gcaccttctc agcgacgata tcgccggcga cagtgttggt
     3901 cagcggtacc tcggccgtag gaatcagcca gcggccatca gtcgtgcgga aattgtcctc
     3961 ggcgaatttg ggcaactggc cg
//
LOCUS       DATGEM010000502         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1400580, whole genome shotgun sequence.
ACCESSION   DATGEM010000502 DATGEM010000000
VERSION     DATGEM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400580"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VKQ27_15520"
     CDS             <1..321
                     /locus_tag="VKQ27_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY90390.1"
                     /translation="EGQEEAAASLGLHWLHTQALIVLPQALRIIVPGIINTVVDLFKD
                     TTLITIIGLLDLLGTVEQALKDPAWLGFAAEGYVFSAAVFFLCCFAMSAYGRNMERRL
                     ARRQ"
     gene            complement(332..970)
                     /locus_tag="VKQ27_15525"
     CDS             complement(332..970)
                     /locus_tag="VKQ27_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY90391.1"
                     /translation="MGQLRGGIVPVTGFQQNCTILWDDDSKQGVVTDPGGDVDRILSA
                     IEQAGVSVGQIWLTHGHLDHAGGAAELAEKLGGVPIEGPDRRDEFLLQKLEEQARAYG
                     FSMRNVAPTRWLVEGDHVAFGEHRFDVLHCPGHTPGHVVYVNHPAKFALVGDVLFQGS
                     VGRTDFPYGDHAALITAIKEKLLPLGDDLQFLCGHGPGSSIGAERAHNPFLQ"
     gene            1047..1352
                     /locus_tag="VKQ27_15530"
     CDS             1047..1352
                     /locus_tag="VKQ27_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90392.1"
                     /translation="MRVRRRSVHPSSMPDHHFRTLDRLIQQANQTSARKPGSVEILAT
                     MIRLATEDGSDPYLILGVLAESAVHVLDKHVPPERRAEAAAEFSRLLLERLKARGLA"
     gene            complement(1457..2431)
                     /locus_tag="VKQ27_15535"
     CDS             complement(1457..2431)
                     /locus_tag="VKQ27_15535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HLY90393.1"
                     /translation="MKNQDWEIPPNLQPDADDYSFDLERALKSIVNLKSYVPADAFTA
                     GTLGTERAGSGVVIRENGLVATIGYLITEAETIWITSSDGRAVPGHALAYDQETGFGL
                     VQALGQLNLPALEMGDSDRLKVGDQVVFAAGGGKHHAVETRVVGRQEFAGYWEYVLDD
                     AIFTAPAHPFWGGGGLIGSDGKLMGIGSLILQQGDGKGRRLDMNMVVPIGLLTPIMKD
                     LLTYGRVNRPARPWLGMYATEADDSIVVGGLADNGPAEQAGVQQGDRILAVGGDEVGD
                     LGGLWRRVWASGSAGAEVRLRLMRDKTPVALTVRSGDRASFLKSPKLH"
     gene            complement(2523..2852)
                     /locus_tag="VKQ27_15540"
     CDS             complement(2523..2852)
                     /locus_tag="VKQ27_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLY90394.1"
                     /translation="MAFGRELKRRAKAVAAPLVFLSIVAYFGWNVVQGDHGLRAYARR
                     QALLVQAQADQAKAQADRDAWDLRVSGLRSRHIDADALDERARAMLNLADPTDVIVQY
                     GPSDKLF"
     gene            2951..3499
                     /locus_tag="VKQ27_15545"
     CDS             2951..3499
                     /locus_tag="VKQ27_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90395.1"
                     /translation="MTTRILSASLLATLALAACTPPPAPDRFFTPQDLSELVTNRTLA
                     VAGEPGGSLLYLAANGTGWLARDVRPGWPPTPGDMSMVFAWRLEGPSRICYWATPRIG
                     AMPDFAPASLECLQILRSSDTAGALTGIVERDQAPPRSRPLEVYAYSLFPPSVIDQYL
                     TQVRVLYGGHIPAWTVPVPLPG"
     gene            complement(3518..3844)
                     /locus_tag="VKQ27_15550"
     CDS             complement(3518..3844)
                     /locus_tag="VKQ27_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90396.1"
                     /translation="MRLPLPLAAACSFAVALQAPIAVAASSTAKSHADQTVEQRFTRA
                     NTAHDGHLTLDQAKAGYPTIARHFDAIDRDKKGYVTGDDIRAYYKTQRTLHRPAASAA
                     HKEPRS"
     gene            complement(3904..>4359)
                     /locus_tag="VKQ27_15555"
     CDS             complement(3904..>4359)
                     /locus_tag="VKQ27_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HLY90397.1"
                     /translation="CDSVWVDFTKGLGAPLGAVLCGSEEFIDAAWRWKQRLGGSLRQA
                     GVCAAACIYALDHNIDRLADDHANARALAQGLAQIPGITVEKPETNLVFFDTTGTGLT
                     ADQLAQRVRREGITLSTTGRYRARACTHLDIDHGGMEVALQAIRRALAN"
BASE COUNT          754 a         1528 c         1269 g          808 t
ORIGIN      
        1 gaaggtcagg aggaagccgc tgcctcgctc ggcctgcact ggctgcatac acaggccctg
       61 atcgttctgc cacaggcatt gcgtatcatc gtgcctggca tcatcaatac cgtcgtagac
      121 ctgttcaagg acacgacgct gatcacgatc atcggccttc ttgatcttct tggcacggtg
      181 gaacaggcgc tgaaggaccc agcctggctg ggctttgcag cggaaggcta tgttttctcc
      241 gccgcggtgt tcttcttgtg ctgcttcgcg atgtcggcgt acggccgcaa catggagcgg
      301 cggctcgcaa gacgccaatg agccgtggcg atcactgcaa gaacggattg tgtgcccgct
      361 ccgccccgat cgaggaccca ggcccgtgcc cgcacaggaa ctgtaagtca tcacccaaag
      421 gcagcagttt ctccttgatg gcggtgatca gagccgcgtg gtcgccataa ggaaaatccg
      481 tccgcccaac cgagccctgg aacagcacgt ctcccaccag cgcgaacttc gccgggtggt
      541 tcacataaac gacgtgtccc ggcgtatgcc ctggacagtg caacacatcg aaccgatgct
      601 cgccgaacgc cacatggtcg ccctccacca gccaccgcgt cggcgccacg ttgcgcattg
      661 agaacccgta ggcgcgcgcc tgctcctcca gcttttgcaa cagaaactca tcgcgccggt
      721 ccggcccttc aatcggaacg ccgcccagct tttccgccag ttccgctgcc ccgcccgcat
      781 gatccagatg cccatgcgtc aaccagatct gcccaaccga aacgcctgcc tgctcgatcg
      841 cactcagtat gcggtccacg tcgccgcccg ggtcggtcac aacgccctgc ttactgtcat
      901 catcccacag gatggtacag ttctgctgaa atccggtaac ggggacgata cccccgcgta
      961 gctgtcccat gacgtcggtt ctcgcggttg ctggccgaaa actgaaggat agcgccctgt
     1021 tcgactgtgt gcacttgcgg ccgctcttgc gcgtccgccg gcggtccgtt catccttcct
     1081 ccatgcccga ccaccatttc cgcacgcttg accgtctgat ccagcaggcc aatcagacat
     1141 cagcccgcaa gccaggttcc gtcgagattc tggccaccat gatccgcctc gcaacagagg
     1201 atggctctga tccatacctt attctcggtg tgctcgcgga aagcgcggtt cacgtcctgg
     1261 acaagcacgt cccgcccgaa cgccgcgccg aggcagccgc agaattcagc cgtctcctgc
     1321 tcgaacgtct caaggcgcgt ggcctggcct gattctgcgc gccctgacga tcgtataagc
     1381 gtcgtttggc gcaccgggtg ctgaagaaat cgacgcaagc gagagcgcgg gcccgaagga
     1441 ataggccccg ctgcgcttag tgcagcttcg gcgacttcag gaaacttgcc cgatcgcctg
     1501 accgcaccgt cagcgccacc ggcgttttgt cgcgcatcaa gcgcagccgg acctcagctc
     1561 ccgcgctgcc cgatgcccaa acgcggcgcc acagtccacc caggtcgccg acttcgtcgc
     1621 caccaaccgc caggatccgg tctccctgtt gtacacccgc ctgctcggca gggccgttgt
     1681 ccgccagccc gcccacgacg atggagtcat ccgcctcggt ggcatacatt cctagccatg
     1741 gccgcgccgg tcgattcacc cgcccatagg tcagcaggtc tttcatgatc ggcgtcagca
     1801 gcccgatcgg cacgaccatg ttcatatcca gccgccgccc cttgccgtcg ccctgttgca
     1861 ggatcagcga gccgatcccc atcagcttgc catcagatcc gatcagcccg ccgccgcccc
     1921 agaatggatg cgccggagct gtgaagatcg cgtcatcaag cacatattcc cagtatcccg
     1981 cgaactcctg ccgccccacc acgcgcgtct ccacggcatg gtgcttgccg ccaccggccg
     2041 cgaacactac ctggtctccc accttcagcc ggtcggaatc ccccatctcg agcgccggca
     2101 gattgagctg gccgagcgcc tgcaccagac cgaacccggt ctcctggtca taggccaaag
     2161 catgccccgg caccgcgcga ccgtccgagg atgtgatcca tatcgtctct gcctcggtga
     2221 tcaggtagcc gatggtcgcg accagaccgt tctcacggat caccacgccg ctgcccgcgc
     2281 gctcggttcc cagcgtcccc gcggtgaacg cgtccgccgg cacatagctc ttcagattga
     2341 cgatcgactt gagcgcccgc tcaaggtcga acgagtagtc atctgcgtcc ggctgcagat
     2401 tgggcgggat ttcccagtcc tgattcttca tgcctcgacg tcttccctgg ttcgaaggcg
     2461 gggcgctcat gccgatacta tccgcccttt ggccgtccgt ggccagagag caattggggc
     2521 ggtcagaaca acttgtcgga cggcccgtac tgtacgatca cgtccgtggg gtccgcgagg
     2581 ttcagcattg cccgtgcacg ttcgtccaga gcgtccgcgt cgatgtgccg cgagcgcagg
     2641 ccggatactc tcagatccca tgcatcccgg tccgcctgcg ccttggcctg gtctgcctgc
     2701 gcctgcacca gcaacgcctg gcgccgtgcg taggcccgaa gcccatgatc gccctgcacc
     2761 acgttccagc cgaaataggc gacaatcgat aagaaaacca gtggcgccgc cactgctttc
     2821 gccctgcgct tcaattcacg tccgaaggcc atcaactgct ccaccttccg gctggacatg
     2881 ctacaggatt tcccgcgcgt cggaaacgcc gctgtcgtcg cgcacacagc atcggatcga
     2941 gtgttgccac atgaccaccc gcatcctttc ggccagcctt ctcgcaaccc tggcgcttgc
     3001 ggcctgcacc ccaccgccgg cgccggaccg gttcttcact cctcaggatc tgagcgaact
     3061 ggtgaccaac cggaccctcg cggttgccgg cgagcctggg ggctcattgc tctatctcgc
     3121 cgccaacgga accggctggc tggcgcgcga cgtccgaccc ggctggccgc ccacccccgg
     3181 tgacatgtcc atggtgttcg cctggcgcct agagggtcct tcgcgcatct gctactgggc
     3241 gacgccgcgc atcggcgcaa tgccggactt cgctcccgcc tcactcgaat gtctgcaaat
     3301 cctgcgcagc tccgacacag cgggtgcact tacgggcatt gtcgagcggg atcaggcccc
     3361 ccctcgctcc cgtccgctgg aggtctatgc ctacagcctg tttccacctt ccgtgatcga
     3421 ccagtatctg acccaggtga gggttctcta tggcggccat attcccgcct ggacggtccc
     3481 agttccgttg cccggataag agcgatgcgc ggcgtggcta actcctcggt tccttgtgag
     3541 ccgcggatgc cgccggccga tgcagcgtgc gctgggtctt gtaataggcg cggatgtcgt
     3601 ccccggtcac gtagcccttc ttatccctgt cgatcgcatc gaaatgccgg gcgatcgttg
     3661 gataaccggc cttggcctga tccagcgtca gatgaccgtc atgcgccgta ttggcgcggg
     3721 tgaagcgctg ttccacagtc tggtcggcgt ggctcttcgc ggtggaagat gcagcgaccg
     3781 cgatcggcgc ctgcagggct acggcaaaac tgcaggctgc ggcgagcggc aacggcaggc
     3841 gcatcgggga gcctttgcaa tggaacgggc caccacaatg ggccaggcac tgctaaccca
     3901 tcgctaattt gccagcgcgc gacggatagc ctgcagcgcg acttccatac cgccatggtc
     3961 gatatccaga tgcgtgcagg cacgggcacg atagcggccg gtcgtcgaaa gggtgatgcc
     4021 ctcccggcgg acgcgctggg caagctggtc cgcggtgagg ccggtgccgg tggtgtcgaa
     4081 gaacaccagg ttggtttcgg gtttctccac cgtgatgcca gggatctgcg ccaggccctg
     4141 ggcgagggca cgggcgttcg catgatcgtc cgcaaggcgg tcgatgttgt ggtcgagcgc
     4201 gtagatacaa gcggctgcac agacaccggc ctggcgcagc gagccgccga gacgctgctt
     4261 ccagcgccag gcagcgtcga tgaactcctc cgagccgcac agcacggcgc ccagcggcgc
     4321 gccgaggcct ttggtgaaat cgacccagac ggaatcgca
//
LOCUS       DATGEM010000503         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_29279, whole genome shotgun sequence.
ACCESSION   DATGEM010000503 DATGEM010000000
VERSION     DATGEM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29279"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VKQ27_15560"
     CDS             <1..663
                     /locus_tag="VKQ27_15560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLY90398.1"
                     /translation="ADRVARLLLTASGGAFRDRPASALSAVTPSEALQHPTWSMGPKI
                     TIDSATLMNKGLEVIEAHWLFGLGFDHIDVVIHHQSVIHSMVAFVDGSVKAQLGLPDM
                     RLPIQYAMAHPRRLSAPVEQLDLAAVGSLTFAPVDAAKYPCLGLAYAAGRAGGTCPTV
                     LNAANEVAVARFLANDLAYVDIAHLIDAALQAHKSRSNPALEEVEAADAWARTFARGW
                     AR"
     gene            728..1792
                     /locus_tag="VKQ27_15565"
     CDS             728..1792
                     /locus_tag="VKQ27_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HLY90399.1"
                     /translation="MNFLWFLPIISLMMFVHELGHYVTARLSGITVQEFGFGLPPRLW
                     GYTAKNGVIYSINWIPFGAFVKMLGEEDPTAPGSFASKSRPIRAIVLAAGSGMNFLLA
                     VAAFAMVFVVGIPGITPDSPVTVAEIADASPAQAAGLQPGDVIVAVDNQPVTVQSFRD
                     ATQTHLDQPMPIQYKRGDQTIATTLTPRSAPPDGQGAIGIVISGGLVRLNPLAAVGQG
                     FMQTVRVIGLTLSVPKMLLEGTISPSDARLVGLVGMAQETSATVSYVADTGFWYPLFF
                     LTGLFSAGLSIANMLPIPALDGGRLFFVLLEWIRGRRIPPEREAAYHFVGIVVLLTIM
                     VIISFNDLISPLPAINWAGR"
BASE COUNT          375 a          727 c          645 g          409 t
ORIGIN      
        1 gcggaccgcg ttgcccgcct cttgctgact gcctcgggtg gcgccttccg cgatcgaccg
       61 gcctccgcgc tttccgccgt cactccgtcg gaagcgttgc agcacccgac ctggagtatg
      121 ggccccaaaa tcaccatcga ctccgccact ctgatgaaca agggcctcga agtcatcgaa
      181 gctcactggc tgtttggcct tggcttcgac cacatcgacg tggtgatcca ccaccagagc
      241 gtcatccact cgatggtggc cttcgtggac ggctcggtca aagcgcagct cggtttgcct
      301 gatatgcgcc tgccgatcca gtacgcgatg gcgcacccgc ggcgcctgtc ggcacccgtc
      361 gagcagcttg atctcgccgc cgtcggctcg cttacgttcg ctcccgtgga cgcggccaag
      421 tacccgtgtc tggggctggc gtacgcggct ggccgcgcgg gcggaacctg tccaaccgtg
      481 ctgaatgccg cgaacgaagt tgccgtcgcc cgctttcttg ccaatgacct tgcatacgtc
      541 gacatcgccc acctgatcga cgccgccttg caagctcata agtctcgctc caatccggca
      601 ctggaagaag tagaggcggc tgacgcctgg gcgcggacct tcgccagagg ctgggcccgc
      661 taatagctgg gctttaacaa ctcgattgtt atgatccccg ggcggaaacc agcatccgcc
      721 aagccaaatg aacttcctct ggttcctccc catcatcagc ctgatgatgt tcgtccacga
      781 gttgggccac tacgtcacgg ctcgcctgtc aggcatcacc gtccaggaat ttggctttgg
      841 cttgccgccg cgcctgtggg gctacacggc caaaaacggc gtgatctact cgatcaactg
      901 gattcccttc ggcgccttcg tgaagatgct cggcgaagag gaccccaccg ccccaggcag
      961 tttcgcgagc aagtcgcgcc cgatcagggc aatcgtgctg gcggcgggca gcgggatgaa
     1021 cttcctgctc gcggtagcgg cgttcgctat ggtgttcgtc gtcggtatcc caggcatcac
     1081 cccggatagc cccgtgaccg ttgccgagat cgccgacgcc agtccagccc aggcagccgg
     1141 gctgcaaccc ggcgatgtga tcgtcgccgt ggacaaccaa cctgtcacgg tccagagctt
     1201 ccgcgatgcc acgcaaaccc atctcgacca gccgatgccc atccagtaca agcgcggcga
     1261 ccagaccatc gcgacgaccc tcacgccgcg ttcggcgcca ccagacgggc agggcgccat
     1321 cggcatcgtc atctcaggcg gtctggtgcg cctgaacccg ttggctgccg ttgggcaggg
     1381 cttcatgcag acggtgcgcg tcatcggact cacgctgagc gtgccgaaga tgctgctgga
     1441 aggcacgatt tcgccgagcg acgcgcgact cgtgggcctc gtcggtatgg cacaggagac
     1501 ctcggccacc gtcagctatg ttgccgacac aggcttctgg tatccgttgt tcttcctgac
     1561 cggcctgttc agcgcgggac tgtcaattgc caacatgctg ccgatcccgg cactcgacgg
     1621 cgggcgcctc ttcttcgtcc tcctggagtg gatccgtgga cggcgtatcc cacccgagcg
     1681 cgaagccgcc taccactttg tgggtatcgt ggtgctgctc acgatcatgg tcatcatctc
     1741 gttcaacgac ctgatctcgc cgttgcccgc catcaactgg gcagggcgtt aaaagttgaa
     1801 ccgggtttga gggcggagcc ctcaggaact gtcggtcgac cagttggcgg ccgatactgt
     1861 acgcagaaca tgttgctagc cagtccgcaa tacaccacca gtcggttcgc ctatcgacgt
     1921 cgcaagtcgc gcgaggttca gataggcagt gtcgccgttg gcggcaacca gcctattcgc
     1981 gtccaatcga tgaccaccac gcgcacccag gacatcgagg gtacgctggc ccagaccgtc
     2041 cgtctggtgg aggcgggctg cgagatcgtc cgcatcactg cgccaaccac ggccgatgcc
     2101 aaagccatag gcgaagtgaa gcgccgcctg cgggcgatgg gcatcgacgt gccgct
//
LOCUS       DATGEM010000504         7970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1283942, whole genome shotgun sequence.
ACCESSION   DATGEM010000504 DATGEM010000000
VERSION     DATGEM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283942"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..730)
                     /locus_tag="VKQ27_15570"
     CDS             complement(104..730)
                     /locus_tag="VKQ27_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLY90400.1"
                     /translation="MRVLITSIIVAPLVLGSLPAFAQSNPSASQIINSLKPSGNLVMG
                     GTRGIRLAAPTDGAQPSPPQAAAKPAATPQTHVASSKPMAPAAASSASGPAVSLTVNF
                     ANGSADLTPDAIKSLDTLGQALSSKDLAGYKFRIEGHTDTVGSPTYNKELSERRAEAV
                     VDYIAKKFSVEPTRLQAVGMGEEGLLVQTPAQTPEPRNRRVQVINLGA"
     gene            complement(815..2329)
                     /locus_tag="VKQ27_15575"
     CDS             complement(815..2329)
                     /locus_tag="VKQ27_15575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90401.1"
                     /translation="MSGWGGGYVTDITYMTGYYRTQTPSLMSLACLLGSVAVPLPGPD
                     DEVSYLELGCGQGFGALLLAASNPRWTVTAIDLHPAHIATAREWAAKAQIANIRFLEA
                     DLSTLAEDPASAKVPEADVVSFHGLWSWVPRAVQAGVVRLLGQKVKSGGVVHISYNAL
                     PAWGPALGMQRLMREGGRRLCAGRSDQQVVEGLKLVQELRAVDAYQLARSIWLKNLIE
                     RTEKLPVHYLAHEYMNESWAPCFHADVAEAMEGAKLEWVASGNLPENFPELTLSAEQR
                     AIASRFDDPVLRELVKDMCLNRSLRHDVFVRGARRISPAMRDAMLRDIWLTLSIAPGE
                     LPSQVEMPAGDAELPADYYQPIADALGSGPRRVGDLLRLPALEGKRDNPAELVGVMVG
                     LNMAEPALRPGAQPVPQATRFNRLITAELSRGENPGRQLAAASHMLGAGAPCSLFDLY
                     VLERVQMGEGEANLDDWVRQTAGNMEEDARAKLRQVFTRCLQVRVPVMRAQGVT"
     gene            complement(2326..3375)
                     /gene="tssG"
                     /locus_tag="VKQ27_15580"
     CDS             complement(2326..3375)
                     /gene="tssG"
                     /locus_tag="VKQ27_15580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HLY90402.1"
                     /translation="MTSRSALALLGSMPRRFRFDAAVRVLARARRTQDPAEAVRFRSA
                     PGLVYPPADVLEVRQQGDALPEVTVGLMGLTGPSGVLPRYYTEVLTQTLRSRSAALRD
                     FFDMLSHRFVAFFAGSGAKYRPARAAESAAQEKAADPVSQVLLALTGYGTPHLTERLA
                     AGSEPLLHYAGLFAMRPRSADRLGALISDWLGMAVEVQEFAGAWLSLPPEQRTRLSAH
                     GAWCRLGVDAAAGVRAWDPQARIILRIGPLDLAAFQRLLPDRVALHRLVSLVRAYVGF
                     ELGFAINPVLAARQVPPLLLNAAADPPPLLGWNTWIPGPAGGFTTRGDAADAVFEAEV
                     IEAQQVDWRGTGQTT"
     gene            complement(3372..5192)
                     /gene="tssF"
                     /locus_tag="VKQ27_15585"
     CDS             complement(3372..5192)
                     /gene="tssF"
                     /locus_tag="VKQ27_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HLY90403.1"
                     /translation="MSDSLLPYYERELDAIKRLAAEFADTHPKIAGRLRLSADAVDDP
                     HVARLLEGVAFLAARVQHRLDDEFPELTDALLSLLYPHYLAPVPSCMVVQFDCQPELM
                     TPSHLASGMALDTEQVRGETLRYRTTAPVTLWPVEVENVRLSGLPLVAPANPAAAGAS
                     GVLRITLKCANPEATFTQLGVDRLRFFLRGPANQTLALYELLGAHAVSVAYADSSVDP
                     APVIVPATAIQPVGFSPEEALLPWSARGFSGFRLLTEYFAFPDKFLFFDFTRIDAKTL
                     VSAGNRLEIFVYLDRSLPELERTIGQQSMALGCTPVVNLFPQRCEPITLNHTDTEYRI
                     VPDARRPRAAEVWNVERVREVLPDGSFRPWRAFHRLSDNGVEAGTPGGSYHIARRDSA
                     PGVPGTESFLAPHDPDFDPDRPADSVLSIDALCVNRDLPTDLPFGGGHPRLRLVEGSA
                     AVAAINAVTAATPTLRPPLREKGYWRLVSHLSLGHLSVTGGREGASSLKEVLRLYDLR
                     DTAETRVAIEALTSVSAGPGTARAPGRAGGFCRGLDVTLEFDPRNWQAGGLYLLAAVL
                     EHFLALHGTVNSFTRTRVTLRGRPGVAAAWPARSGTRVLA"
     gene            complement(5315..5830)
                     /gene="tssE"
                     /locus_tag="VKQ27_15590"
     CDS             complement(5315..5830)
                     /gene="tssE"
                     /locus_tag="VKQ27_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HLY90404.1"
                     /translation="MSGRTGPHTRGTTTRVQAPLLDRLIDDAPDQDRDPVVSAADSMV
                     ALRLSVRRDLEALLNARRRWRSWPARMTQLNTSPLSYGIPDFASGAFNDSARREQLRI
                     EIEDTIRRFEPRFLSVRVQLVDAKERLETTLRLRIEAVMHAEPAPEAVTFDTLVDPTT
                     DDVTVRPGNVV"
     gene            complement(5827..6630)
                     /locus_tag="VKQ27_15595"
     CDS             complement(5827..6630)
                     /locus_tag="VKQ27_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HLY90405.1"
                     /translation="MSGTDQSAAALYRAGKLDEAVAAAQAAVRKAPTDLNARVLLGEL
                     LAFAGNLERADVVLDAASAIDPTAALVVAEFRQLIRADMARRQLFRDGRVPELLSDAT
                     ETQRLQLAALVALRGGDIAEAAKQAAAAEEARPRVSGKHGDTAFDDWRDADDLLAGSF
                     EVLTTTGKYFWIPTERVASLEFHAPKRPRDLLWRRASMSVSEGPDGDVYIPVVYATDE
                     AMTDPLRLGRETDWKEAEGAPVRGIGQRLFMAGEEAIPLMDLGNITFGA"
     gene            complement(6659..>7970)
                     /gene="tssC"
                     /locus_tag="VKQ27_15600"
     CDS             complement(6659..>7970)
                     /gene="tssC"
                     /locus_tag="VKQ27_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HLY90406.1"
                     /translation="DRDIAAIDAMLSAQVDAILHHARLRKLEGTWRGIAWLVDNSDLS
                     NRLKLKLLNVGWPEICRDLERAIEFDQSQLFRKVYEEEFGTPGGEPYGLLIADHEVRH
                     RSTAEFRTDDVNALGLLSGVAAAAFSPVIVGASPALLEVDNFADLAMTVDVAASLRNA
                     EHARWRSLASRADMRFVGVALPHVLARPPWEDDGTRADGFRYAEYAPSSDERVWMNAG
                     YPFASVVMRAFAQYAWPADVRGVETDRVGGGLVTDVPIESFRTDPERVWTRSAVEIVL
                     TDRQERALLDAGLMPLSSIPYSDELVFGAVRSLQLPQRYQGRTADAAEANARLSTQIN
                     SMLCASRFAHNLKMKGRHMVGSFKTADEIERFLQSWLTKYVSGNLSAGPETLARYPLV
                     DGKVTVNEIPGKPGSFGCVIHLQPYYQLDDMSAAFQLVTDLTGR"
BASE COUNT         1419 a         2731 c         2548 g         1272 t
ORIGIN      
        1 caatcagcgg cgcgccggaa cggcgagcac gcgacgatat ccggatcgtg tcgtctgcga
       61 gcggatcttg cggcgtcata cgtcagccgc gatgcggcgt gactcaggcg cctagattga
      121 tcacctggac ccggcgatta cgcggctcgg gagtctgtgc aggggtctgc accagcaggc
      181 cttcctcgcc catgccgacg gcctgcagcc gcgtgggctc gacgctgaat ttcttggcga
      241 tgtaatcgac caccgcctcg gcccggcgct ccgacaattc cttgttgtat gtgggagagc
      301 ccactgtgtc ggtgtgccct tcgatgcgga atttatagcc ggcaaggtct ttgctgctga
      361 gcgcctggcc caatgtgtcc agcgacttga tcgcatccgg cgtcagatct gccgaaccat
      421 tggcgaagtt caccgtcagg ctgactgcag ggccactcgc cgacgacgcg gccgccggcg
      481 ccattggctt gcttgacgcc acgtgtgtct gtggcgtggc ggctggtttc gccgcggcct
      541 gcggtggcga cggctgcgca ccatcggtgg gagcggcgag ccggataccg cgtgtcccgc
      601 ccattaccag attgccgctg ggcttcagcg aattgatgat ctgcgaagcc gatgggttgc
      661 tctgggcgaa tgccggcagg ctgccgagaa ccaacggagc tacgataatc gaggttatca
      721 gcacacgcac gggacacgcc ctcccaagtc tgaaattcgc cgccttatat catgtaccgc
      781 gccaaatgcg aggatttgtc cgggccggca acgcttatgt cacgccctgt gcgcgcatta
      841 ccggcaccct tacttgcaag caacgggtga atacctgccg aagtttggcg cgtgcgtctt
      901 cctccatgtt gcccgctgtc tgacgtaccc agtcgtccag gttggcctcg ccttcgccca
      961 tctgcacgcg ctccagcacg tagagatcga acaggctgca gggggcgccg gcacccagca
     1021 tgtgactcgc tgccgccagc tgccggccgg gattttcgcc gcggctcaat tcagcagtga
     1081 tcagccgatt gaaacgtgtg gcctggggca ctggctgtgc gcctggccgc agcgcgggct
     1141 ccgccatgtt cagcccaacc atgacgccaa ccagctcggc cgggttgtca cgcttgccct
     1201 ccagtgccgg cagtctcagc agatcaccga cacggcgcgg cccgctgccc agtgcgtctg
     1261 cgattggctg gtaatagtca gcgggcaact cggcgtcacc ggcgggcatc tcaacctgtg
     1321 acggcaactc gcccggagca atgctcaacg tcagccagat gtcgcgcagc atcgcgtcgc
     1381 gcatcgccgg cgaaatccgg cgggcgccgc gcacgaagac atcatgccgc agggagcggt
     1441 tcaggcacat gtccttcacc agttcgcgca gcaccggatc atcgaaccgg ctggcgatgg
     1501 cccgctgctc agccgacagc gtcagctccg ggaagttctc cggcaggttg ccagaggcga
     1561 cccattccag ctttgcgccc tccatcgcct cggcaacgtc ggcgtgaaag cagggcgccc
     1621 acgactcgtt catgtattca tgcgcgaggt aatgcaccgg cagcttttcg gtgcgttcga
     1681 tcaggttctt cagccagatt gagcgggcaa gttgatacgc atcgacggct cgcagttcct
     1741 gcacgagctt caggccctcg accacctgct gatcgcttcg gccggcacac aggcggcggc
     1801 cgccttcgcg catcagccgc tgcatgccaa gcgcgggtcc ccaggcgggc aacgcattgt
     1861 agctgatgtg cacaacgccg cctgacttca ccttctgccc cagcagcctg accacaccgg
     1921 cctgaacggc acgtggcacc cagctccaca ggccgtggaa gctgacgaca tcggcttccg
     1981 gcactttggc ggaagccgga tcctcagcca gcgtcgatag atcggcttcc aggaaccgga
     2041 tgttggcgat ctgcgctttg gccgcccact cgcgcgccgt ggcgatatgc gccgggtgca
     2101 agtcgatcgc ggtcaccgtc cagcgcggat tgctcgccgc gagcagcagt gcgccgaacc
     2161 cctggccaca gcccagctcg aggtagctca cctcatcgtc cggccccggc agcggaacgg
     2221 ccacactgcc gagcagacag gcaagcgaca tcagcgatgg cgtctgagtg cggtagtaac
     2281 ccgtcatgta cgtgatatca gtcacgtatc cgccgcccca tccgctcatg ttgtttgtcc
     2341 tgttccgcgc caatcgacct gttgcgcctc gatcacctcg gcctcgaaca ccgcatcggc
     2401 tgcgtcgccg cgcgtggtga agccgcccgc cgggcccggg atccaagtgt tccagcccag
     2461 cagcggcggt ggatccgccg ccgcattcag cagcagcggc ggcacctgcc gtgccgcgag
     2521 gactggattg atggcaaagc ccagctcgaa gccgacatag gcgcgcacca gcgaaaccag
     2581 ccggtgcagt gccacccggt cgggcagcag gcgctggaat gctgcgaggt ccaacgggcc
     2641 gatccgcagg atgatccgcg cctgcgggtc ccaggctcgc acgccagctg cggcatccac
     2701 gccgaggcgg caccaggcac cgtgcgccga tagccgcgtt cgctgctcgg gtggcaacga
     2761 gagccacgct ccggcgaact cctgcacctc caccgccatg cccagccagt ccgagatcag
     2821 cgccccgagc cggtcagccg accgcggccg cattgcaaac aggccggcgt aatgcagcag
     2881 cggctcgctg ccggcggcca gccgctcggt cagatgtggg gtgccgtacc cagtgagcgc
     2941 caacagtacc tgcgacaccg gatccgccgc cttctcctgc gcggctgact cagccgcgcg
     3001 tgcgggacga tacttcgcac cacttccggc aaagaacgcc acgaaacggt gtgacagcat
     3061 gtcgaagaag tcccgcagcg ctgccgaacg cgaacgcagg gtctgggtaa gaacctccgt
     3121 gtagtagcgg ggcagcacgc cagaggggcc agtcagcccc atcaggccga cggtcacctc
     3181 aggcaacgca tcaccctgct ggcgtacttc cagaacgtcc gccgggggat agacaaggcc
     3241 gggcgccgac cggaaccgca ccgcttcggc ggggtcttgc gtccgtcgcg ctcgggcgag
     3301 cacccgaacc gccgcatcga agcggaagcg tcgcggcatg ctccccagca acgccagcgc
     3361 gctgcggctt gtcatgccag cacgcgcgta ccactgcgtg ctggccaggc ggccgcgacc
     3421 ccgggacggc cccgcagagt gacgcgggtc cgtgtgaagc tgttgaccgt gccgtgcagc
     3481 gccaggaagt gctcgagcac agcagccagc aaatagagcc ccccggcctg ccagttgcgc
     3541 ggatcgaact ccagcgtcac atccagcccg cggcagaagc cgccggcacg gcccggtgcc
     3601 cgcgcagtac cggggcccgc gctcacgctg gtgagtgcct cgatcgcgac gcgcgtctcg
     3661 gcggtgtcgc gcaggtcgta aagccgcagc acctccttca gggagctggc accttcgcgc
     3721 ccgccggtca ccgagaggtg gccgagcgac aaatgagaca ccagccgcca gtagcctttc
     3781 tcgcgcagcg gcgggcgcaa ggtgggtgtc gcggcggtga ccgcgttgat cgccgccacc
     3841 gcagcggatc cctcaacgag acgcagacgc ggatggcccc caccgaacgg caggtcagtt
     3901 ggcaggtcgc ggttgacgca cagcgcatcg atcgagagaa cggaatcggc tggccggtca
     3961 ggatcgaagt cgggatcatg aggtgccagg aacgattctg tacccggcac gccgggtgcc
     4021 gaatcgcgac gcgcaatgtg gtacgagccg ccgggcgtgc ccgcctccac gccgttgtcc
     4081 gacaggcggt ggaatgcccg ccaggggcgg aacgagccgt ccggcagcac ctcgcgcacc
     4141 cgctccacat tccacacctc tgccgcacgc ggacgccggg catccgggac gatgcgatat
     4201 tcggtgtcgg tgtggttcag tgtgatgggc tcgcagcgct gcggaaacag gttgaccacc
     4261 ggcgtgcagc caagcgccat cgactgctgg ccgatcgtgc gttccagctc cggcagggag
     4321 cggtccaggt aaacgaagat ctccagtcgg ttgcccgccg agaccaaggt cttggcgtcg
     4381 atccgggtga aatcgaagaa caggaacttg tcgggaaaag cgaagtattc ggtgagcaga
     4441 cgaaagccgg agaagccgcg ggccgaccat gggagcagcg cttcctcggg cgagaacccg
     4501 acaggctgga tcgcggtcgc gggcacgatc accggcgcag gatcgaccga gctgtcggca
     4561 taggcgaccg acaccgcgtg cgcgccgagc aattcataca gcgccagcgt ctggttcgcc
     4621 ggcccgcgca ggaaaaaccg caggcgatcg accccgagct gtgtgaaagt ggcctccggg
     4681 ttggcgcatt tcagggtgat gcgaagcacg ccgctggcgc cggctgctgc cggattggcc
     4741 ggcgcgacga gcggcaggcc agacaggcgc acattctcga cctcgacagg ccacagcgtg
     4801 accggcgccg tggtcctgta gcgcaacgtc tcaccacgca cctgttcggt gtcgagcgcc
     4861 atgcctgaag caaggtgcga tggggtcatc agctccggct ggcagtcgaa ctgcaccacc
     4921 atgcaagagg gaacgggtgc caggtaatgt ggatacagca ggctcagcag cgcatcggtc
     4981 agctccggga attcatcatc cagtcggtgt tgcacacggg cggcaaggaa cgcgacgccc
     5041 tccagcaggc gggcaacgtg cgggtcgtcc accgcgtctg ccgatagccg gagccgcccc
     5101 gcaatcttcg gatgtgtatc tgcgaattcg gccgccagac gcttgatggc gtccagctcg
     5161 cgctcataat aaggcagcag tgaatcagac atcgaaataa gaaccgcgaa acgcttctcc
     5221 gtcatggccg gtgacgaccg gccatccaca tctttgaagc atcaaaggaa ggcgcggacg
     5281 gccggcctct atccgccacg acggtgcgaa cccttcagac cacattgccc ggccgcaccg
     5341 taacatcgtc cgtcgtagga tcgaccagcg tgtcgaacgt cactgcctcc ggcgccggtt
     5401 cggcatgcat caccgcctcg atccgcagcc gcagtgtcgt ctccagccgc tccttcgcgt
     5461 caaccagctg cacccttacc gaaaggaacc gcggctcaaa ccggcggatc gtgtcctcaa
     5521 tctcaatccg aagctgctcc cgccgcgccg aatcattgaa cgcgcccgaa gcgaaatccg
     5581 gaatcccata actgagcggc gacgtattga gctgcgtcat gcgcgctggc cacgaccgcc
     5641 agcgccgccg cgcattcagc agcgcttcaa ggtcccgccg cacggacagg cgcagcgcaa
     5701 ccatcgaatc agccgccgaa accactggat cacgatcctg gtcgggcgca tcgtcgatca
     5761 gccgatcgag cagcggcgcc tgaacgcgcg tggtggtgcc gcgtgtgtgt gggccggtgc
     5821 gtccgctcat gcgccgaacg tgatgttgcc gagatccatc agtggaatcg cttcctcgcc
     5881 cgccatgaac aggcgctgcc cgatgccgcg caccggggcg ccttctgctt ccttccagtc
     5941 tgtctcccgc cccagacgca gcggatccgt catcgcttcg tcggtggcat acacgaccgg
     6001 tatatacaca tcgccatccg gaccctccga caccgacatc gacgcacggc gccacaacag
     6061 gtcgcgtggg cgcttcggcg cgtggaactc cagcgaggca acgcgctcgg tcgggatcca
     6121 gaaatacttg ccggtcgtgg tgagcacttc gaagctgccg gcaagcagat catccgcatc
     6181 gcgccagtcg tcgaacgccg tgtcgccatg cttgccggag acacggggac gcgcctcctc
     6241 ggccgccgcc gcctgcttcg cggcctccgc aatgtcgccg ccgcgcagcg ccaccagcgc
     6301 ggccagttgc agccgctggg tttcggtggc gtccgacagg agctcgggca cgcgcccgtc
     6361 gcggaacaac tgccgccgcg ccatgtcggc gcggatgagc tggcggaatt ccgcgaccac
     6421 cagtgcggcg gtcggatcaa tggccgaggc ggcgtccagc accacgtcgg cgcgctcgag
     6481 attgccggca aaggccaaca gttcgcccag caggacgcgg gcgttcagat cggtgggcgc
     6541 cttgcgcaca gccgcctgcg ccgccgccac agcctcatcg agcttgccgg cgcgatacag
     6601 cgcggcagca gattggtcgg ttccgctcat gctttcctcc agatgcgccg gcttgcgctc
     6661 atcgtccggt gagatcggtg acgagttgga acgcggcgga catgtcgtcg agttggtaat
     6721 agggctgcag atggatcacg cagccgaagc tgccaggttt gccaggaatc tcgttgaccg
     6781 tgaccttgcc gtcgaccagc gggtagcgcg cgagagtttc gggccccgcc gatagattgc
     6841 cgctcacgta cttggtcagc caggattgca ggaagcgctc gatctcgtcg gccgtcttga
     6901 aagaccccac catgtgtcgg cctttcatct tcaggttatg cgcgaagcgc gacgcgcaca
     6961 gcatcgagtt gatctgcgtg gaaagccgcg cattggcctc ggccgcgtcg gcggtgcggc
     7021 cctgatagcg ctgcggaagc tgcaggcttc gcaccgcgcc gaacaccagc tcatcgctgt
     7081 acggaattga ggacagcggc atcagaccgg catcgagcaa ggcgcgctcc tgccggtcgg
     7141 tcagcacaat ctcgaccgcg ctgcgcgtcc agacgcgctc cgggtcggtg cgaaatgact
     7201 cgatcggcac atcagtgaca aggccgccgc ccacgcgatc ggtctccacg ccacgcacgt
     7261 ccgcgggcca cgcatattgt gcgaaggcac gcatcaccac cgaggcaaag gggtagcccg
     7321 cgttcatcca gacgcgttcg tcactgctcg gcgcatattc cgcatagcga aagccgtcag
     7381 cccgggtccc atcgtcttcc cacgggggtc gcgccaggac atgcggcagg gcgacgccga
     7441 caaagcgcat gtcggcccgc gacgccaggc tgcgccagcg cgcatgctcc gcgttccgca
     7501 acgacgcggc gacgtcaacc gtcattgcca gatcggcgaa gttgtccacc tcgagcagtg
     7561 ccggcgacgc gcctacgatc accggcgaga atgccgccgc ggcgacaccc gatagcagcc
     7621 cgagcgcgtt gacatcgtcg gtccggaatt cggcggtcga acgatgccgc acttcgtggt
     7681 ccgcgatcag caggccatat ggctcgcccc ccggggtgcc gaactcctct tcataaacct
     7741 tgcggaagag ctggctctgg tcgaactcga tggcgcgctc caggtcccgg cagatttcgg
     7801 gccagccgac attgagcagc ttcagcttca ggcggttcga cagatcggag ttgtcgacca
     7861 gccaggcgat accgcgccac gtgccttcca gcttgcgcag ccgcgcgtgg tgcaggatgg
     7921 cgtccacctg cgccgacagc atcgcatcga tcgccgcgat atcgcgatcc
//
LOCUS       DATGEM010000505         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1170845, whole genome shotgun sequence.
ACCESSION   DATGEM010000505 DATGEM010000000
VERSION     DATGEM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170845"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VKQ27_15605"
     CDS             complement(<1..281)
                     /locus_tag="VKQ27_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HLY90407.1"
                     /translation="MTLDPSRISFCASPTALAAEAHARLVARYGDCPLEKASVVVPLG
                     GDGFMLETIHRVLPLDLPVYGMNCGSVGFLMNTFNEDDLPERIIQGQEAV"
     gene            complement(278..1114)
                     /locus_tag="VKQ27_15610"
     CDS             complement(278..1114)
                     /locus_tag="VKQ27_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLY90408.1"
                     /translation="MRLSVVQMNPGADKDENIAQARRLIDEAVQADRPNIVSLPEVWD
                     SLGGDRTVRQSNAELLPAKGSNEPGGPAYEFLRDTARSHKVHVHGGSIIEKRSEKLFN
                     TTVVFDPDGQEIARYRKMHLFDIVTPDGTGYRESNTYGAGDEVVTYEAGDTKVGCAIC
                     YDLRFPDLFWKLREQGAEVIFLPSAFTLATGKDHWEVMIRSRAIETQCWFAAPATWGK
                     HLEGKGEARFTYGHSMIVDPWGHVTAKVSDGTGWATSRIDHKVTARVRRDMPVLEHRK
                     RV"
     gene            complement(1179..1574)
                     /locus_tag="VKQ27_15615"
     CDS             complement(1179..1574)
                     /locus_tag="VKQ27_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90409.1"
                     /translation="MTLPFTLPDWLPWWVPIVLLVPALLYGLAFLFMPFSVIGVKSRL
                     DVIEARLDEIQGEIRHLALRLPEVSRAADYDEIYPPEPPVQSRRAPPSISRPPIPPAS
                     HELDDRDSPGARDRRPSGGRSEPKLDWPR"
     gene            1763..2878
                     /locus_tag="VKQ27_15620"
     CDS             1763..2878
                     /locus_tag="VKQ27_15620"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HLY90410.1"
                     /translation="MLLLIPGPVTTRPEVREALRYDFAPWDNDFRPLYAGVRERVLRV
                     AGGIPGEHATLPLQGCGHFITEAVVRTFIPPGGKLLIPGTGAYADRMIRLAREAGRVP
                     VVMPMSPVVATDPDAVAMALATDPEIRHVGMVYSETGSGIVHDVAAVGAVARRLGRRM
                     LVDAVSAFGALPLDLSAHPEIDCVGFTSNKCLEGVPGMAFAVARVDSVLACEGNAGSW
                     SFDLQSIYAHATRSGWGSFRFTPPAQVLNALNVALDFFDAEGGQVARLARYTSNMRTF
                     YEGMRGLGLTPCLPAHIQGPIIVNVHAPDDPRWDLQGFVDALKRRGVLISNFYNTETP
                     SFRVGCIGAVTPADMSRAVTAMGETLKEMGIEQRKAA"
     gene            2943..3269
                     /locus_tag="VKQ27_15625"
     CDS             2943..3269
                     /locus_tag="VKQ27_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90411.1"
                     /translation="MPQGTGAPFIYLVQMDIPAEHEADFNRVYDTEHVPEILKVPGVR
                     ACSRYVLERTNKTGLARYLALYEVDSAEVIDSPAWTKAAEIGDWAPKIRPHTTNRSHT
                     VMRKIS"
     gene            3284..>4509
                     /locus_tag="VKQ27_15630"
     CDS             3284..>4509
                     /locus_tag="VKQ27_15630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HLY90412.1"
                     /translation="MKSDLEIARESKLRPIAEIAAKLSIPDDALEPYGRHKAKIGTRF
                     IASLEGKPDGALVLVTGISPTPAGEGKTTTTVGLGDALNRIGMRAAICLREPSLGPSF
                     GMKGGAAGGGYSQVVPMDQINLHFTGDFHAITSANNLLAAMIDNHIYWGNSLGIDARR
                     VSWRRCMDMNDRSLRSIVGSLGGVANGFPREDGFDITVASEVMAVFCLSKNLDELQER
                     LGKMIVAQKRDGSAVTAKDLKADGAMAVLLKDALAPNLVQTLEGSPAFVHGGPFANIA
                     HGCNSVMATRLGLKLGDVVVTEAGFGADLGAEKFIDIKCRNAGLKPSCAVVVATVRAL
                     KMHGGVARNALGTENVEAVKKGVANLARHVENLGKFGLPVVVGVNHFTSDTEAEFAAI
                     REAMAKQGVEAISCTH"
BASE COUNT          833 a         1330 c         1546 g          800 t
ORIGIN      
        1 acagcctcct ggccttggat gatgcgctct ggcagatcgt cctcattgaa ggtgttcatg
       61 aggaagccga cggagccaca gttcatgccg taaaccggca gatctaacgg cagcacgcga
      121 tggatggttt ccagcatgaa tccgtccccc ccaagcggaa cgacgaccga tgctttctcc
      181 aacggacaat caccgtagcg cgcgacgagg cgggcgtgcg cttccgccgc cagtgcggtg
      241 ggcgaggcgc agaacgagat ccggctggga tcgagggtca aacgcgcttc cggtgttcca
      301 gcaccggcat gtcgcgacgt acgcgcgcgg tgaccttgtg atcgatgcgc gaggtggccc
      361 aaccggtgcc gtccgacact tttgccgtca cgtgtcccca gggatcgacg atcatcgaat
      421 ggccataggt gaagcgggcc tctcccttgc cttcgaggtg cttgccccag gtggcgggcg
      481 cggcgaacca gcactgcgtc tcgatcgcgc gggaacggat catcacctcc cagtggtcct
      541 tgccagtggc gagcgtgaat gctgatggca ggaagatcac ctcggcgcct tgctcgcgca
      601 gcttccagaa caggtcgggg aagcggagat catagcaaat ggcgcagccc actttggtat
      661 cgccggcctc gtaggtgacc acctcatcgc cggctccata ggtgttgctt tcgcgatagc
      721 cggtaccgtc aggcgtgaca atgtcgaaca gatgcatctt gcgatagcgc gcgatctcct
      781 gtccatcggg atcgaatacc acggtcgtgt tgaacagttt ttccgacctc ttctcgatga
      841 tcgaaccgcc gtggacgtgc accttatggg accgagcggt gtcacgcaga aactcgtagg
      901 cgggaccgcc aggttcattg gaacctttcg ccggcagcag ctccgcgttg gattgccgca
      961 cggttcggtc gccgccaagg ctgtcccaca cttcagggag cgagacgata ttgggacggt
     1021 cggcctggac agcctcatcg atgaggcggc gggcctgggc gatgttctcg tccttgtccg
     1081 cacctgggtt catctgaacg acactgagac gcatcggcgg ctccgggtgt ggtgatcggc
     1141 ttagagaaga accgaagcgc ggcagggacg caaccgcctc agcgcggcca gtcgagtttg
     1201 ggctcggaac ggcccccact ggggcggcgg tcacgcgcgc caggcgagtc ccggtcgtcc
     1261 agttcatgcg atgcaggcgg gattggcggc cggcttatcg acggcggcgc ccggcgcgac
     1321 tgcacagggg gttccggggg atagatttca tcgtagtcgg cggcgcgcga gacttcggga
     1381 agacgcagcg ccagatgtcg gatttcaccc tggatttcgt cgagccgcgc ctcgatcacg
     1441 tcgaggcggc ttttcacccc gatcacgctg aacggcatga acaggaaggc cagcccgtaa
     1501 agcagcgcag gcacaagcag gacgatcggc acccaccagg gaagccagtc cggcagcgtg
     1561 aagggaagcg tcatgcgtgg accttagcat tccgcgtgcc catcttccat ccgacaccgg
     1621 ggaggcagga ttggcgagga gcgatatcac gctgtaacag gcggcaacaa agggatcggg
     1681 ttcgagacct gtcggcggag cgggtggatt tccgtcccgc accgtggcaa gccttcgggt
     1741 ccgatcgttc caggtgcgct gcatgttgtt gttgatcccc ggccccgtca ccactcgccc
     1801 tgaggtgcgc gaggccctgc gttacgactt cgcaccgtgg gacaatgact tccgtccact
     1861 ctatgccggc gtccgcgagc gggtgctgcg cgttgccggc ggcattcccg gcgagcacgc
     1921 tactctgccg ctgcaaggct gcggccactt catcaccgag gcggtggtac gcacattcat
     1981 cccgccgggc gggaagctgc tgatcccagg aaccggcgct tacgccgatc ggatgatcag
     2041 gctcgcgcgt gaggcaggac gggtgcccgt ggtgatgccg atgtcaccgg tcgtggcgac
     2101 cgaccccgat gcggtggcta tggcgctcgc aaccgatccg gagatccgcc atgttgggat
     2161 ggtgtacagc gagaccggta gcggtatcgt ccacgatgtc gcggcagttg gcgcggtcgc
     2221 gcgccggttg ggacggcgga tgctggtgga cgcggtttcc gcgtttggtg cgttgccgct
     2281 cgacctgtcg gcgcatcccg agatcgattg tgtcgggttc acttccaaca aatgcctgga
     2341 gggcgtcccg ggcatggcat tcgccgtggc gcgcgtggac agcgtgctgg cctgcgaggg
     2401 gaacgctgga agctggtctt tcgatctgca aagcatctat gcacacgcga cacgcagcgg
     2461 ctggggcagc ttccggttca caccgcctgc gcaggtgctg aacgcgctca acgtcgcgct
     2521 ggatttcttc gacgccgagg gcgggcaggt agcgcgtctg gcgcgctata cctcgaacat
     2581 gcggaccttt tatgaaggta tgcgcggcct tgggcttacg ccgtgtcttc cggcacatat
     2641 acaagggccg atcatcgtga atgtgcacgc accagatgat ccacgctggg acctgcaagg
     2701 gtttgtcgat gcgctgaagc gacgcggcgt gttgatcagc aatttttata acacagagac
     2761 gccgagcttc cgcgtgggat gcattggcgc ggtgacgccg gccgatatgt cgcgcgccgt
     2821 gactgcgatg ggcgagacac tgaaggagat ggggatcgag caacgcaagg cggcctgacc
     2881 tggtcgccgg cgcctcctcg gaatgacggg gtgatgcgac gatagaacgg aggagacaag
     2941 agatgccgca gggcacaggt gcaccattca tctacctggt gcaaatggat atccccgccg
     3001 aacacgaggc ggatttcaat cgcgtctatg acaccgagca tgtgccagag atcctgaagg
     3061 tgcctggcgt gcgcgcctgc tcgcgctatg tgctggagcg gaccaacaag acggggctgg
     3121 cgcggtatct cgccttgtat gaggtcgact cagcagaggt gatcgacagc ccggcctgga
     3181 cgaaggcggc cgagatcggc gactgggcgc cgaagatccg cccgcacacg accaatcggt
     3241 cgcacactgt gatgcgcaag ataagctgag ggaaccaaga gacatgaaaa gcgacctgga
     3301 gatcgcccgc gaatcaaagc tccgcccgat cgcggaaatc gccgccaagc tgagcattcc
     3361 ggatgatgcg ctggagccgt atgggcgaca caaagcgaag atcggcacgc gcttcatcgc
     3421 gtccctggaa ggcaagccgg atggcgcgtt ggtgttggtg accggcatca gcccgacgcc
     3481 ggcgggcgaa ggcaaaacca ctaccacggt cgggctgggc gatgcgctga accgtatcgg
     3541 catgcgcgcg gcgatctgcc tgcgcgagcc ctcgcttggg ccgagctttg ggatgaaagg
     3601 tggtgccgcg ggcggtggct attcgcaggt cgtgccgatg gaccagatca acctgcattt
     3661 caccggcgac ttccacgcca tcacgtcggc caacaacttg ctggcggcga tgatcgacaa
     3721 ccacatctac tgggggaatt cgctgggcat cgacgcgcgg cgcgtgtcgt ggcggcggtg
     3781 catggacatg aacgatcgct cgctgcgatc gatcgtcggc agcctgggtg gcgtggccaa
     3841 cggcttccct cgcgaggatg gcttcgacat cacggtcgcg tccgaggtga tggcggtctt
     3901 ctgcctgtcg aagaacctcg acgagcttca ggagcggctc ggcaagatga tcgtcgcgca
     3961 gaagcgcgac gggtccgcgg taacagcgaa ggatctgaag gcggacggcg cgatggccgt
     4021 gctgctgaag gacgctctgg cgcccaacct cgtgcagaca ctggagggtt cgccggcgtt
     4081 cgtgcacggc ggcccattcg ccaacatcgc gcacggctgc aattcggtga tggccacaag
     4141 gcttgggctg aagctaggag atgtggtggt gaccgaagcc ggctttggcg ccgacctggg
     4201 tgcggaaaag ttcatcgaca tcaagtgccg caacgcaggg ctgaagccat cctgcgccgt
     4261 ggtggtggcg accgtgcggg cgctgaagat gcatggcggt gtggcgcgca atgcgctggg
     4321 taccgagaat gtcgaggcag tgaagaaagg cgtagcgaac ctggcgcgac acgtggagaa
     4381 cctgggcaaa ttcggcctgc cggtggtggt gggggtgaac cacttcacgt ccgacaccga
     4441 ggctgagttc gcggcgatcc gggaagcgat ggcgaagcag ggcgtggagg ctatctcttg
     4501 cacgcattg
//
LOCUS       DATGEM010000506         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1199523, whole genome shotgun sequence.
ACCESSION   DATGEM010000506 DATGEM010000000
VERSION     DATGEM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199523"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..947)
                     /locus_tag="VKQ27_15635"
     CDS             complement(42..947)
                     /locus_tag="VKQ27_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLY90413.1"
                     /translation="MPLWIPITVAAALFQCWRTAMQQKLRHLLSVNGAGFVRFLYGAP
                     TAFVLLVVSLAATGLPLPAPNALFVAHCAAGGLTQILATNLLIMSFGFRNFAVGTAYS
                     KTETAQSAIVALIVLGEVLRPSAWIGICIGLAGVMTLSLAGRGMRPRELLAATVQPAA
                     LCGLGAGFLFALTTVFIKLANHALTGPSLFVRALFVLVVTNTLQTLMQGGFLAWREPQ
                     ELRKAITTWRSSSWVGTLSALGSACWFTAFALTDVALVRSVGQIEIVFTLLFSRFYLR
                     ETLRPGDIAGLVLVVCGVLLIVISS"
     gene            complement(1058..1468)
                     /locus_tag="VKQ27_15640"
     CDS             complement(1058..1468)
                     /locus_tag="VKQ27_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HLY90414.1"
                     /translation="MSDYTKLTPQPTPDTQPYWDGLNAHVLRLQRCADCAKVRHYPRP
                     VCDACFSMRVEWIDASGRGRVHSWTTTHHPFHPGFRPDLPYTLLTVDLEEGVRMNAQL
                     RNLDPADLRVGLPVRVDFEPATEGLTLPVFVPDR"
     gene            complement(1465..2607)
                     /locus_tag="VKQ27_15645"
     CDS             complement(1465..2607)
                     /locus_tag="VKQ27_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLY90415.1"
                     /translation="MSLQEVGAVTGVGETQYMRGTDRTPLSLQLEASLRAIDDAGLKP
                     TDIDGVIPYGHGAVAEDFVTNFGIADLRFSATTPMGGASAVAAIQCAIAAIGAGICNH
                     VLLSIGRTGYSGGRIGTRVQQMPQFRLIGEFEMPSGAIAPAQLYAVMARRHMELYGTT
                     SRQFAEIAVSTRRHASLNPNATMRNPITIEDHQSSRVISDPLRLLDCSLESDGGAAVV
                     ISAADRARDLRHRPITVMGVAEGHPDSPSAITQRPDLTTLGLAKAAPKAFAMAGVTHS
                     DIDVAEVYDCFTYIVLCQLEDLGFCKKGEGGGFVQDGAISLGGKMPVNTHGGLLSQAH
                     MAGMNHIVELTRQLRGEAAAQVPDAEIGLVTGYGDMGDGSIAIMRR"
     gene            complement(2659..3249)
                     /locus_tag="VKQ27_15650"
     CDS             complement(2659..3249)
                     /locus_tag="VKQ27_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HLY90416.1"
                     /translation="MANRHELVAADILPAAEYAAHRKDWRSKIGAIKRNRRVEVGPYV
                     TFYFESYDTMWLQVQEMVYIEKGGADQVPGELEAYNPLIPKGDELCATFMIEIDDPDR
                     RHRVLGTLGGIEETVLIRVGDHTVRAAAEADQDRTTAEGKASSVQFVHFHFTPAQIAE
                     FSRHGAQVILGIEHRNYGHMAVLPEAVRAELAKDFR"
     gene            complement(3261..4031)
                     /locus_tag="VKQ27_15655"
     CDS             complement(3261..4031)
                     /locus_tag="VKQ27_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HLY90417.1"
                     /translation="MNTAAPAHGRKAVLYATCFVNYNTPGVGIATQAVLARNGVETVV
                     VHPHCCGMPMLEQGRIDEVAKNARTVAGAMREWIAKGYDIVALVPSCALMLKFEWPLI
                     LPNDPDVQALSKATYDISEYVVDISRKEGLADGLQPIDGGITLHMACHSRAQNIGQKA
                     AEMLRLLPEADVQVIERCSGHGGSWGVKKGNFEVALKVGRPVARQARDAAKAFVTSEC
                     PLAGVHIVQGIEKLGGDAPKPALVTHPIELFARSYGIG"
BASE COUNT          730 a         1277 c         1345 g          728 t
ORIGIN      
        1 gcgcatcgaa gaggtggatc cggccgtggt cgcctcgatg ttcacgaact gatcacgatc
       61 agcagcacgc cgcagaccac caggacgagg cctgcaatgt cgcctggccg cagcgtttca
      121 cgcaggtaga agcggctgaa cagcagggtg aacacgatct cgatctgacc gaccgagcgc
      181 accagcgcga cgtcggtcag cgcgaaggcg gtgaaccagc aggcggagcc gagcgcggac
      241 agcgtgccca cccaggatga actgcgccag gtggtgatgg ccttgcgtag ctcctgcggt
      301 tcgcgccacg cgaggaaacc gccctgcatc agggtttgca gcgtgttggt cacgaccagc
      361 acgaacaggg cgcgcacgaa caggctgggt ccggtcagcg cgtggttcgc cagcttgatg
      421 aacactgtcg tcagcgcgaa caggaagcct gcaccgaggc cgcacagcgc ggcaggctgc
      481 acggtggctg cgagcagttc acgtggccgc atcccgcggc cggcgagcga caaggtcatg
      541 acccccgcga gcccgatgca tatgccgatc catgcactgg ggcgaagcac ctcgccgagg
      601 actatcagtg cgacgatagc gctctgcgcg gtttcagtct tggaatatgc tgtgccgacg
      661 gcgaagttac ggaatccaaa cgacatgatc agcaggttgg tggccaggat ctgcgtcagg
      721 ccgccggccg cgcagtgggc gacgaacaat gcgttgggtg ccggtagtgg cagaccggtt
      781 gctgccagcg agacgaccag cagcacgaat gctgtcggcg cgccgtagag gaaccgcacg
      841 aagccggcgc cgttcaccga aagaaggtgt cgcagtttct gctgcatggc ggtgcgccag
      901 cactggaaca gcgcggcagc gacagtgatc ggaatccaaa gcggcatgcc acgtctccct
      961 cgggcgcggc aggcgcgcgt caattcatgg tcggatcagg ctgaccacaa ccctcgaata
     1021 tcgttccggg acagtaaacc aagcacaggt gcagcgatca acggtccgga acgaataccg
     1081 gcagcgtgag gccttcggtg gccggctcga agtcgacgcg caccggaagg ccaacgcgca
     1141 ggtcggctgg atccaggttg cggagctgcg cgttcatccg cacaccttcc tcgagatcga
     1201 cggtcagcag cgtgtacggc aggtcggggc gaaagcctgg atggaaggga tgatgggtcg
     1261 tggtccagct gtgcacgcgg ccacggccac tcgcatcgat ccactccacg cgcatcgaga
     1321 agcatgcatc gcagaccggg cgggggtaat gccgtacctt cgcgcaatcg gcgcagcgtt
     1381 gcagacggag cacatgcgcg ttgagcccgt cccagtaggg ttgcgtgtct ggcgtcggct
     1441 gcggtgtcag cttggtgtag tcgctcatcg ccgcatgatg gcgatcgagc catcccccat
     1501 gtcgccatag ccggtaacca agccgatctc ggcatcgggc acctgggcgg ccgcctcgcc
     1561 gcgcaactgg cgggtgagtt cgacgatgtg gttcatgcct gccatatgcg cctgcgacag
     1621 caggccgcca tgcgtgttca ccggcatctt gccgccgagg ctgatcgctc cgtcctgcac
     1681 gaagccgccg ccttcgccct tcttgcagaa accgagatcc tccaactggc agagcacgat
     1741 ataggtgaag cagtcataga cctcggcaac gtcgatatcg ctgtgcgtga cgccggccat
     1801 cgcgaatgcc tttggcgcgg ctttcgcgag accaagcgtt gtcagatcgg ggcgctgggt
     1861 gatggcgctt ggggaatcag ggtggccttc cgccacgccc atcacggtga tggggcggtg
     1921 ccgcaggtcg cgcgcgcgat ccgccgcact gatcacgacc gcggcgccgc cgtcgctctc
     1981 cagcgagcaa tcgagcaatc ggagtggatc ggaaatcacg cgagaggact ggtgatcctc
     2041 gatcgtgatc ggattgcgca tcgtcgcatt gggattgagc gaggcatggc gccgcgtgga
     2101 tacggcgatc tcggcgaact ggcgggatgt ggtgccgtac agctccatgt gccgccgggc
     2161 catcacggca tagagctgtg ccggcgcgat ggcgccagag ggcatctcga attcgccgat
     2221 cagacggaat tgcggcatct gctgcacacg tgtgccgatg cgcccgccgg aatagccggt
     2281 gcgtccgata gacagcagca cgtggttgca tatgccggcg ccgatcgcgg caatcgcaca
     2341 ctggatggcg gcaaccgcac tcgcgccgcc catcggggtg gtcgcggaaa accgcagatc
     2401 ggcgatgccg aaattggtga cgaagtcttc cgcgacggcg ccatggccgt atgggatcac
     2461 gccgtcgatg tcggtgggct tcaggcccgc atcatcgatc gcgcgcagcg atgcttccag
     2521 ttgcagcgaa agcggcgtgc gatccgtgcc acgcatatac tgcgtttcac cgacgccggt
     2581 tacggctccc acttcctgca gggacattga cgctatcctc catcgtcacg cggctcttcg
     2641 ccttgggcga cgatggcgtc agcggaagtc cttcgcaagc tcggcgcgca ccgcctcggg
     2701 caggacagcc atgtggccgt agttgcgatg ctcgatcccc aggatcactt gcgcgccatg
     2761 cctggagaat tcagcgatct gcgccggtgt gaagtggaaa tgcacaaact gcaccgagga
     2821 tgccttgcct tccgccgtgg tgcggtcctg atcggcttcg gccgcggcgc ggacggtgtg
     2881 atcgccaaca cggatcaaca ctgtctcctc gatcccgccc agcgttccaa gcacgcggtg
     2941 gcggcgatcc gggtcatcga tctcgatcat gaaggtggcg cagagttcgt caccttttgg
     3001 gatcagcgga ttatacgctt cgagttcgcc gggtacctga tcggcaccac ctttctcgat
     3061 gtagaccatc tcctgaacct gcagccacat cgtgtcgtag ctctcgaagt agaacgtcac
     3121 gtatgggccg acttcgacgc ggcggttgcg cttgatcgcg cctatcttgc tgcgccagtc
     3181 cttgcgatgc gctgcgtatt ccgccgcggg gaggatatcg gctgcgacaa gttcatgccg
     3241 gttcgccatg ctggcctcct tcagccgatg ccgtaggaac gcgcgaaaag ctcgatcgga
     3301 tgcgtgacca gcgcgggctt cggtgcatcg ccgccgagct tttcgatgcc ctgtacgatg
     3361 tgcacgccgg ccagcgggca ttccgaggtg acgaacgctt tcgctgcatc tcgggcctgg
     3421 cgggcgacgg ggcggccgac tttcagcgcg acctcgaagt tgcccttctt cacgccccag
     3481 gagccgccat ggccggagca gcgttcgatc acctgcacgt cagcttccgg caacaggcgc
     3541 agcatttccg cggccttctg gccgatgttc tgtgcgcggg agtggcaggc catgtgcagg
     3601 gtgatgccgc cgtcgatcgg ctgcaggcca tcggccaggc cttccttgcg gctaatgtcc
     3661 accacgtatt cgctgatatc gtaagtggcc ttcgacagcg cctgcacatc aggatcgttc
     3721 ggcaggatga gcggccattc gaatttcagc atcagcgcgc aggacgggac gagcgcgacg
     3781 atgtcgtagc ccttggcgat ccattcgcgc atcgcgccgg caacggtgcg ggcgttcttt
     3841 gcgacttcgt cgatgcggcc ctgttccagc atcggcatgc cgcagcaatg cggatggacg
     3901 acgacagtct ctacgccatt cctggccagc accgcctgtg tcgcaatgcc gacgccgggc
     3961 gtgttgtagt tgacgaagca cgtcgcgtag agcacggcct tccgtccgtg tgccggcgcc
     4021 gcggtgttca ccgcaggcgc agactttgcg gccgaggcga aggtcttcgc atggtatttc
//
LOCUS       DATGEM010000507         8052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_99418, whole genome shotgun sequence.
ACCESSION   DATGEM010000507 DATGEM010000000
VERSION     DATGEM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99418"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VKQ27_15660"
     CDS             complement(<1..832)
                     /locus_tag="VKQ27_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLY90418.1"
                     /translation="MAARSLTGPIPGFSEERPVWFGRRQVTERQVATAEAEAPPQAPR
                     LPPSAAIVELRALCLGEMDPTALAKLTPEELTQEVERTLAEIATQRRIQLNGREQRAL
                     ARELVNDMLGLGPLEPLLEDDTITDIMVNGPEKVFVEKRGKLELSTVRFRDAGHVAHV
                     CQRIAVRVGRRIDESTPMVDARLKDGSRVNIVFPPLALDGPYISIRKFSRNRINFAKL
                     VEFGSLSTHAAHLLEMAARARLNVIISGGTGSGKTTLLNALSSLIDIGERIVTIEDAA
                     E"
     gene            complement(814..2064)
                     /locus_tag="VKQ27_15665"
     CDS             complement(814..2064)
                     /locus_tag="VKQ27_15665"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HLY90419.1"
                     /translation="MQHDVPHAEMPLEQAPARPDRKDIVAFVTDAETESVVRDGLSDS
                     APRGIEFHRTNIRGAVDILQNTATPRVLVVDVTGEPRPLTALEGLSDVVEPDVQVLLI
                     GEFNDVNFYRHATRILGVKEYLFKPITRDMVARHFGPVILNRSRRPETMQGGRVVTVT
                     GVRGGVGASTIATNLAWYLGKRANRHTILVDADLYRGNCALLLNTKSGPGLRTALETP
                     QRVDELFVERSAQPVTERLHVLSGEENLTDHVKYEVGAAARLIETLRRRYNFIVIDAP
                     FSGLELHRDLLMLGHQRILVMEPNLVGIRDALRLLALPNGPLQVHRGLLVLNRANRPG
                     MLALRQIEEALKMSVDIIIPDLPKLVEDAANIGEPAALQSRFNKAIVALAGQATAAFP
                     IDSSPESPSEAATRRWRRLWPLAA"
     gene            complement(2064..2360)
                     /locus_tag="VKQ27_15670"
     CDS             complement(2064..2360)
                     /locus_tag="VKQ27_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90420.1"
                     /translation="MLTRLLPSILALLVLAGCDATDPYMRDGMWRPNEANETNLRAMV
                     ASPADLVRGMPSEGNGQQAAAALDRYRNDKVRLLPDSAVAKITPVSSGTQQGSQ"
     gene            complement(2374..3693)
                     /locus_tag="VKQ27_15675"
     CDS             complement(2374..3693)
                     /locus_tag="VKQ27_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HLY90421.1"
                     /translation="MPGQTGISIDAGTGRLIQLSQPAASVFAAEPKVAEVRPASATSL
                     FIFGVSPGRTTVAALDASGKPIAQYDVVVQPSKYGAGEAEAMITRAVPGASVHVDTLQ
                     NGLSITGKVKTPAEADRVMQIVRGFAPAGQSVDNRLSVAGALQVNLRVRIAEMSRNLI
                     RQLGVNWSNLVELGRYAQIGLFTNNPLVNLTRAPNQLSGGYNFNTPGHVVDINGLIDT
                     LSQDELIHVLAQPNLTAISGEAASFLVGGEFPIPVAQQNNEVTIEFKQYGVSLAFVPT
                     VGSDGQIAMKVRTEVSQLTNQGAVQLSSGNSTIQVPALTVRRAETAVELGSGESFAIA
                     GLLQDAVNVTGNGVPVIGDVPVLGALFRSDNFQRNETELVIVVTPYVVRPVANPSMLR
                     LPTDGYRPPSDLERILLLRQTARRADSAQIGNQPTPHIPGNAGFIVQ"
     gene            complement(3816..4673)
                     /gene="cpaB"
                     /locus_tag="VKQ27_15680"
     CDS             complement(3816..4673)
                     /gene="cpaB"
                     /locus_tag="VKQ27_15680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HLY90422.1"
                     /translation="MLARTALFILMAIGLAGFGAVAWFNLRPAPPEHTDVAATSDKLP
                     LLIAARPLRAGTLLKPEDLAVDLRYPQTVPAGGRIDSAEVRNNLLGAMIRRNLAKGEP
                     VLAGDALNPGDRGFLAAVLGAGMRAVTVGVDAVTGLSGLVWPGDRVDLILTQSQEGAD
                     IPPSRRISGETVLHDVRVLAIDRQLMQGATSESPESQAARTVTLEVTPYDAERVVVAA
                     RLGHLSLSVVAVAQASGGADAAPGTQQTGNPVTWGGDVSSALRGGPSSEGTTIRLFQG
                     PTDSKEIKF"
     gene            complement(5007..5177)
                     /locus_tag="VKQ27_15685"
     CDS             complement(5007..5177)
                     /locus_tag="VKQ27_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLY90423.1"
                     /translation="MLEIMRTLLALRFNKRGVTAMEYGLIAALIAVAIIGAVSTVGTN
                     LGNTFDAISAKL"
     gene            complement(5261..5467)
                     /locus_tag="VKQ27_15690"
     CDS             complement(5261..5467)
                     /locus_tag="VKQ27_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90424.1"
                     /translation="MSDAKLAAKNEQAVLSGTIFIIPATGRSLWSQGYFRAAAPGQSK
                     YNTIVFCESATIVRADILFGSSVY"
     gene            5484..6557
                     /locus_tag="VKQ27_15695"
     CDS             5484..6557
                     /locus_tag="VKQ27_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90425.1"
                     /translation="MWGADTGTSIIRKWLGQRRPRGAVVPFEAPALRMARVCDDERDG
                     MIAILRDFANNGSSYIVPWSSLPLMAPMTDHDKALHTAVGEVKAATPAQVRAVVHDLA
                     LSGALGQEAAARETRRASAECSAIADVELVLILHLLDSCGTDLAMLMINGARWRDADA
                     KSAIGAAAAKVGVRRQELHRRIAEFARLLVPLGLVAAGGTVRAGWLRALQNEIECFSQ
                     SIADLPPPPWSDASDYLTTILATSRRTAVLSGLVINMLDYAVLDIAGTIGNWGQERPV
                     LGGAIERLSLMLDEWPALMKSVRDALRGPADDGAKQLRILCSMLPRVPEPEPGTADPS
                     GGTSVSAALAARLSTVCSMLPAL"
     gene            complement(6563..7825)
                     /gene="murA"
                     /locus_tag="VKQ27_15700"
     CDS             complement(6563..7825)
                     /gene="murA"
                     /locus_tag="VKQ27_15700"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLY90426.1"
                     /translation="MDRIRIRGGRPLSGEIAIGGAKNATLPLMAAALLTDERLVLSNV
                     PRLEDVTTLTLLLRQHGIAVEPVGNNGRTLSLGGAITSTEAPYDIVRKMRASFLVLGP
                     LLARAGEARVSLPGGCSIGARPIDLHLKGLEQLGADVVLEGGYINARVKGRLRGATII
                     FPFVSVGATENLLMAASLAEGRTVLVNAAREPEIGDLARCLNAMGARIEGVDSDRLTI
                     DGVARLHGAEHMVIPDRIETGTYACAAAITGGEVMLANARLEYLGSTVRALREAGVEI
                     VEQPCGLHVKRLNGLHGADVMTEPYPGFPTDMQAQFMALMAVADGAAMVTETIFENRF
                     MHVPELNRMGARINVHGTSAIVRGVSTLSGAPVMATDLRASVSLVLAGLAAKGETVVN
                     RVYHLDRGFESVEQKLAACGADIERLTG"
     gene            complement(7980..>8052)
                     /gene="dcd"
                     /locus_tag="VKQ27_15705"
     CDS             complement(7980..>8052)
                     /gene="dcd"
                     /locus_tag="VKQ27_15705"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015395.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HLY90427.1"
                     /translation="CETSYADKAGKYMRQRGVALPKL"
BASE COUNT         1425 a         2756 c         2394 g         1477 t
ORIGIN      
        1 gctcggctgc gtcctcaatg gtgacaatgc gctcgccgat atcgatgagg ctcgacagcg
       61 cgttcagcag tgttgtcttt cccgagccgg ttcccccgga aatgatcacg ttgagccgcg
      121 cccgcgctgc catttccagc agatgcgccg catgggtgct aagcgagccg aattccacca
      181 acttggcgaa attgatccgg ttgcgggaga acttgcggat cgagatgtat ggtccatcca
      241 gcgccagcgg cggaaacacg atgttgacgc gcgagccgtc cttcagccgc gcatcaacca
      301 tcggcgtgct ttcgtcgatc cgccgcccta cgcgcacagc gatccgttga cacacatgcg
      361 ccacgtgccc ggcatcacgg aaccgcacag tggacagctc cagcttcccg cgcttctcga
      421 cgaacacctt ctccggcccg ttgaccatga tgtcggtgat cgtgtcgtcc tcgagaagag
      481 gctccagcgg tccgagcccc agcatgtcgt tgaccagttc gcgcgccaga gccctttgct
      541 cgcgtccatt gagctgaatg cgccgttgcg tggcgatctc ggccagagtc cgttcgactt
      601 cctgggtcag ttcttccggt gtcagtttcg ccaacgccgt cgggtccatt tcgccgaggc
      661 acagagcacg caactcgacg atcgccgcgc tcggcggcag acgtggcgcc tgcggcggcg
      721 cctcggcctc ggccgtcgcg acctgccgct ccgtcacctg ccgacgccca aaccagacag
      781 ggcgctcttc gctaaacccc ggaatgggtc cggtcaggct gcgagcggcc acagacgcct
      841 ccatctgcgc gtcgccgctt cgctcggcga ttcaggcgag ctatcgatgg gaaatgccgc
      901 agtcgcctgt ccggcaagtg ccacgatcgc tttgttgaag cggctttgga gcgctgccgg
      961 ctcgccgata ttggcagcat cctccacgag ctttggcaga tccgggataa tgatatcgac
     1021 actcatcttc agcgcctctt cgatctgccg cagtgccagc atgccgggac ggttcgcgcg
     1081 gttcagcacg agcaagccgc gatgcacctg caatggccca ttcggtagcg ccagcagccg
     1141 caacgcgtcg cgaatgccaa ccagatttgg ctccatcacc aggatgcgct ggtgtcccag
     1201 catcagcagg tcgcgatgca gctccagccc gctgaacggc gcatcgatga cgatgaaatt
     1261 gtagcgccga cgtagcgtct cgatcagccg cgccgctgct cccacttcgt atttgacatg
     1321 gtccgtcagg ttctcctcac cggacagcac atgcagtcgc tcggtcaccg gctgggccga
     1381 gcgctcgacg aacagctcgt cgacgcgctg tggcgtctcc agcgcggtgc gcagaccggg
     1441 ccctgatttc gtattcagca acagcgcgca attcccgcgg tacaggtccg catccacaag
     1501 tatcgtgtgg cggttcgcgc gcttgcccag ataccatgcc aggtttgtcg cgatcgtgct
     1561 ggctcccacg ccgccgcgca ccccggtgac agtcaccaca cggccgccct gcatggtttc
     1621 cggccgccgc gaccgattga gaatcaccgg cccgaaatgc cgcgccacca tgtcgcgcgt
     1681 gatcggcttg aacagatact ccttcacgcc gaggatacgc gtcgcgtgcc gatagaagtt
     1741 cacgtcgttg aactcgccga tcagcagcac ctgcacatcg ggttccacga catccgacag
     1801 cccctccagc gccgtaagcg gccgcggctc gcccgtcaca tcaaccacca gcacccgcgg
     1861 cgtcgcggtg ttctgcaata tgtcgaccgc gccgcgaatg ttggtgcgat ggaattcgat
     1921 gccacgcggc gccgaatcgc tgaggccatc gcgcacgacg ctctcggtct cggcatccgt
     1981 tacaaacgca acgatatcct tgcggtcggg gcgtgcaggt gcctgttcca ggggcatctc
     2041 ggcatggggc acatcgtgct gcatcactga ctgccttgct gcgtcccgct ggacaccggc
     2101 gtgatctttg ccaccgcact gtccggcagc agccgcacct tgtcgttccg atatcgatca
     2161 agtgccgcag ccgcctgctg tccgttcccc tcgctcggca tgccgcgcac gagatccgcc
     2221 ggcgaggcaa ccatcgcccg cagattggtc tcattggcct cgttcggccg ccacatgccg
     2281 tcgcgcatat aagggtcggt ggcgtcgcag ccggccagca ccaacagcgc aagaatactg
     2341 ggcaacaacc gcgtcagcat gcactcgcct ccctcattgc acgatgaagc cggcattgcc
     2401 ggggatgtgg ggcgtcggct ggttcccgat ctgagcgcta tcggcgcgcc gcgctgtttg
     2461 gcgaagcagc aggatgcgct cgagatcaga cggcggcctg tacccatccg tcggcagccg
     2521 cagcatcgaa gggttggcca ctggacgcac cacatacggc gttaccacga tgaccagttc
     2581 cgtctcgttg cgctggaaat tgtccgaccg gaacagcgcg ccgagtaccg gcacgtcacc
     2641 tatcaccggc acgccgttgc cggtgacatt caccgcgtcc tgcagcagac cggcgatcgc
     2701 gaagctctcg ccgctgccca gctccaccgc cgtctccgcg cggcgaaccg tcagcgccgg
     2761 cacctggatc gtcgagttgc cagaggagag ctgcacggca ccctggttgg tgagctggct
     2821 cacctcggtg cgcaccttca tcgcgatctg cccgtccgag ccaaccgtgg gcacgaacgc
     2881 aagactgacg ccatactgct tgaactcgat ggtcacttcg ttgttctgct gcgcgaccgg
     2941 aatcgggaac tcaccgccaa ccaggaaact tgcggcctcg ccactgatcg ccgtcagatt
     3001 cggctgcgcc agaacgtgga tcagctcgtc ctgcgacagc gtatcgatca ggccattgat
     3061 atcgacgaca tgcccgggcg tgttgaagtt gtagccaccc gaaagctgat tcggcgcgcg
     3121 tgtcaggttc accagcgggt tgttggtgaa cagcccgatc tgagcatacc tgcctagctc
     3181 aaccagattg gaccaattca cgccgagttg ccggatcagg ttccggctca tctcggcgat
     3241 gcggacccgc aggttcactt gcagcgcgcc tgccactgaa agtctgttgt cgactgactg
     3301 ccctgccggc gcgaacccgc gcacgatctg catcacgcga tccgcctcgg ctggcgtttt
     3361 caccttgcct gtgatgctca agccgttctg cagcgtgtcc acgtgcaccg atgcgccagg
     3421 caccgcccgc gtgatcatgg cctcagcctc gcctgcgccg tatttggacg gctgcaccac
     3481 cacgtcgtac tgcgcgatcg gcttgccact cgcatccaag gccgcaaccg tcgtgcgccc
     3541 tggcgagacg ccgaatatga acaggctggt cgcacttgcc ggccggacct cggcaacctt
     3601 cggctcggct gcgaacacgc tggctgctgg ctgggaaagc tggatcagcc gtccggtgcc
     3661 agcatcgatg ctgataccgg tctgcccagg catcttgacc gcagcggagg atccagtggc
     3721 cgtcgcagaa acgttcgctc gaccgggtcc aggcgcgccc gttgccagct gatgcagacc
     3781 tagcaagagc ataatgacag ccagtaggtg tttcatcaga acttgatctc tttgctgtca
     3841 gtgggacctt ggaacaggcg gatcgtcgtg ccttcgctgc tcggaccacc gcgcagcgcc
     3901 gaggacacgt cgccacccca cgtcaccgga tttcctgtct gctgcgtgcc tggcgcggca
     3961 tccgcgccgc cgctggcctg tgcgaccgcg accacgctga gcgagagatg cccgagcctc
     4021 gccgccacca cgacgcgttc ggcgtcgtac ggcgttactt ccagcgtgac agtgcgcgcc
     4081 gcctgtgatt caggactttc ggaagtggcg ccctgcatga gttgccgatc gatcgccagg
     4141 acacgaacgt catgcagtac ggtttcaccg gatatgcggc gcgacggcgg tatgtcggcg
     4201 ccttcctgcg actgtgtcag gatcagatcg acacgatcac ccggccacac gagtccggaa
     4261 aggcccgtta ccgcatcaac acccactgtc accgcgcgca tgcccgcgcc cagcaccgct
     4321 gcaaggaaac cgcgatcgcc cggattgagg gcatcgcccg cgagcacggg ctcacccttg
     4381 gccagattgc gccggatcat cgcgccgagc agattgttgc ggacttctgc ggagtcgatg
     4441 cgtccccccg ccgggacggt ttgcgggtac cgcagatcca cagcgaggtc ttcgggcttc
     4501 agcaacgtgc ctgcccgcaa cggcctcgcc gcgatgagca acggcaactt gtcacttgtc
     4561 gccgctacat ctgtatgttc aggcggcgct ggacgaaggt tgaaccaagc aacggcgccg
     4621 aagccagcca gcccgatcgc catcagtata aacaatgcag tgcgagccag cattgttcgt
     4681 tcacctggcc gaccgcgcag ccggatcgtc ggccagttcg tgatgtcggc agatcctcgc
     4741 cgatggaccg gacttctcat ccgtccgtcg tggcggcttt gcgtccagcg ccgaataaaa
     4801 gagttccatg cgattcccta gcgttttgcg aggacgatgc ggtgcggcat tatggcgagc
     4861 gccaaatcat gccgctccgg cccatacgta tgtgcgaggt actggttcgg ctatcggcgg
     4921 acaccagcac ctgcacataa tgtcgatgcc gtcattctta cgacttgcgt accgagcgca
     4981 ggctgctttt ccgcgctcgc gtggcatcac aacttcgctg agatggcgtc gaaggtgttt
     5041 cccagattgg ttccaaccgt ggacacggcg ccgatgatcg ccacagcgat cagcgcggcg
     5101 atcaggccat attccattgc tgtcacacca cgcttattga agcgcagagc caacaaggtc
     5161 cgcataattt cgagcattta tccgctatcc tctgacatag aaaccgggac taaagacgaa
     5221 ggccgacgcc ggcttcgagt tttgttctac cctttcgggg ttaataaaca cttgagccga
     5281 acaaaatatc ggcgcgcaca attgtcgccg attcgcagaa tacaattgta ttgtatttgc
     5341 tctggccggg cgcagctgca cggaaatagc cttgcgacca cagagaacgt ccagtcgcag
     5401 gaatgatgaa aatcgtcccg ctgagtacgg cttgttcatt ttttgcagct agcttggcgt
     5461 ccgacattgt ccccagggga atcatgtggg gcgctgacac gggcaccagc atcatccgca
     5521 aatggcttgg ccagcgccgc ccacgcggtg ccgtcgtgcc gtttgaggct ccggcattgc
     5581 gcatggcgcg cgtctgcgac gacgagcgcg atggcatgat cgccattctg cgcgacttcg
     5641 ccaataacgg cagctcctac attgtgccgt ggagcagcct gccgctgatg gcgccgatga
     5701 ccgaccacga caaggcgctg cataccgcgg tgggcgaggt caaggcggcc acccccgcac
     5761 aggtccgcgc agtggtccat gaccttgccc tctcaggcgc cttgggccag gaagccgccg
     5821 cgcgggagac gcgacgcgcc agcgctgaat gcagcgcgat cgccgatgtc gaactcgtgc
     5881 tcatcctgca tctgctggat agttgcggca ccgacctcgc gatgctgatg atcaacggcg
     5941 cacgctggcg tgacgccgac gcaaagtccg ccatcggagc tgccgccgcc aaggtcggtg
     6001 tccggcggca ggaactccat cgccgcatcg ccgagttcgc gcgcctgctc gttccgcttg
     6061 gcctcgttgc cgccgggggc acggtccggg cgggctggct gcgagccctg cagaatgaga
     6121 ttgagtgctt cagccagagc atcgccgatc ttccgcctcc gccctggtcg gacgcgagcg
     6181 attacctcac cactatcctg gcgaccagcc ggagaacagc ggttctgtca ggcctggtga
     6241 tcaacatgct ggactatgcc gtgctcgaca tcgccggcac tatcggcaac tgggggcagg
     6301 aacgccccgt gctaggcggc gccatcgaac ggctatcgct gatgctcgac gaatggcccg
     6361 cgctgatgaa gtcggtacgc gacgcgttgc gtggtccagc tgatgacggg gcaaagcaac
     6421 tccgcatcct ttgctcgatg ctaccgcggg tgccggagcc agagccgggc acagccgatc
     6481 catccggtgg cacatcggtc tccgcggccc tcgctgcccg gctcagcaca gtttgctcga
     6541 tgctgcccgc gttgtgatcg gctcacccgg tcaatcgctc gatatctgcc ccgcatgccg
     6601 cgagcttctg ctcgactgac tcgaaaccgc gatccaggtg gtaaacccgg ttcaccacgg
     6661 tttccccttt ggccgccagc cccgccagca caagcgacac cgacgcccgc agatcggtgg
     6721 ccatcaccgg cgcccccgag agcgtagaca ccccgcgtac gatcgctgac gttccatgca
     6781 cattgatccg cgcccccatc cgattgagct ccggcacgtg catgaagcgg ttctcgaaga
     6841 tcgtctcggt gaccatcgcg gcgccgtccg cgaccgccat gagcgccatg aactgcgcct
     6901 gcatgtcggt cgggaacccc ggatacggct ctgtcatcac gtcggcgccg tgcagcccgt
     6961 tcaggcgctt cacatgcagg ccgcaaggtt gctccacgat ctccacgccg gcttcgcgca
     7021 gagcgcgcac cgtcgagccc agatactcca gccgcgcatt cgccagcatc acctcaccgc
     7081 cggtgatcgc cgccgcacag gcataggtgc ccgtctcgat gcgatccgga atcaccatgt
     7141 gttccgcgcc gtgcaggcgc gccaccccat cgattgtcag ccgatcgctg tcgacgccct
     7201 cgatccgcgc acccatcgca ttcaggcagc gcgcgaggtc gccaatctcc ggctcgcgtg
     7261 ccgcgttcac cagcacggtg cgcccctcgg ccagcgacgc cgccatcagc aggttctcgg
     7321 tcgccccgac ggaaacgaat gggaagatga tcgttgcgcc gcgcagccgc cctttgaccc
     7381 gcgcgttgat gtagccgccc tcgagcacca cgtctgcacc gagttgttcc aaccctttca
     7441 ggtgcagatc gatcggccgc gcgccgatcg agcagccacc cggcagcgac acccgcgcct
     7501 cacccgcgcg cgccaggagc ggccccagca ccaggaacga ggcgcgcatc ttccgaacga
     7561 tgtcatatgg cgcctcggtg ctggtgatgg cgccgcccag cgacaatgtg cggccattgt
     7621 tgcccaccgg ctccaccgca atgccatgct ggcgcagcag cagcgtcagc gtcgtcacgt
     7681 cctcgagccg aggcacgttg ctcagcacca gtcgttcatc ggtgagcagc gcggcggcca
     7741 tcaggggcag tgttgcgttc ttcgcgccac cgatggcgat ctcaccggac agaggccggc
     7801 cgccacggat tcggatacgg tccattcagg ggatccttac gaggtatacg agaaaggtcc
     7861 acacggagag cgcggagcgc cacggaggaa acctggttcc cgctgcgcgc gaagcgctct
     7921 tcggatcact ccgtggttcc ccgtggtcct tcgaaacctc cgcgcggagc ctgcaagact
     7981 catagtttag gtaacgcgac accacgctgg cgcatatatt tgccagcctt atcggcatag
     8041 ctcgtctcgc ac
//
LOCUS       DATGEM010000508         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_800023, whole genome shotgun sequence.
ACCESSION   DATGEM010000508 DATGEM010000000
VERSION     DATGEM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800023"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VKQ27_15710"
     CDS             complement(<1..1051)
                     /locus_tag="VKQ27_15710"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY90428.1"
                     /translation="MPIPLDQLPDDLDALKRIIAAMAQDAVNAQAEIAKLRFQLARYR
                     RAEFGRSSEKLQREVDQLELAIETLEADQAERLAAASPIVAAAIEAAVENKKPARRAL
                     PDHLPREDQIHAAPCACPSCGDALRRIGEDITETLDYVPGRFKVIRHIREKLSCRTCD
                     TVVAAPAPDHAIARGRAGAGLLAHIVVSKYDDHLPLYRQAEIFAREDVELETSTLSGW
                     VGATAAALQPLIDALASDVMTSETLHVDDTPVPVLAPGAGKTKTGRLWTYVRDERPFG
                     GQRPPVALFFYSPDRKGEHPRAHLKDFRGVIHADGYAGFNELFIGNRIVEAGCWAHAR
                     RKFFDVHAAIGSPIAK"
     gene            complement(1122..1469)
                     /gene="tnpB"
                     /locus_tag="VKQ27_15715"
     CDS             complement(1122..1469)
                     /gene="tnpB"
                     /locus_tag="VKQ27_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HLY90429.1"
                     /translation="MIGLPSGARVWLAVGRTDMRKGFDGLAAAVQERMAQDPFCGHLF
                     VFRGRRGDLLKVLWWDGQGLCLFAKRLEKGRFVWPSPADGKVVITPAQLGMLLEGIDW
                     RMPRRTWRPQVAR"
     gene            complement(1466..1834)
                     /locus_tag="VKQ27_15720"
     CDS             complement(1466..1834)
                     /locus_tag="VKQ27_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY90430.1"
                     /translation="MQRSEIITVERRRRWSLSAKQDLVAETLQPGASVSAVAQRHGLH
                     PSQLFAWRKAAREGRLVMDSGEEFVPVVVASAPMSLAPLAPELVPSRMEIVLGNGRRV
                     IVDGTVRASALARVIKVLDR"
     gene            complement(2056..>3038)
                     /locus_tag="VKQ27_15725"
     CDS             complement(2056..>3038)
                     /locus_tag="VKQ27_15725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90431.1"
                     /translation="AKPDEAPAGYMAGFTGVARKREPSPRVDADPAARIADMDYEGVD
                     VNLTLPSGWFGTFTSGDDITLESAMYRAYHRWMSDYCGAFPQRLGGIVLASARDINGS
                     LAEMHHWGKSRWAWGVMVYAPVGVPLDHPSLEPFYALAAELDLAVVLHTFTVMPPYAP
                     GGLDTWDNLWLQRSAAHPWCGMRNMASLIGSGVMDRYPGLRIGTLEAGHGWLPFWIKR
                     LDEHAETIKAALPELRQRPSDYVTSGRYFQSIEIPEGAALTNAVADLLGDDVLMYASD
                     YPHGESHFPKSAEIVTGWAMPEPRKRKLLWDNATKLYTRARAESTQVFRL"
BASE COUNT          542 a         1027 c          941 g          528 t
ORIGIN      
        1 ccttggcgat cggtgaaccg atggcagcgt gtacgtcgaa gaacttgcgc cgcgcgtggg
       61 cccagcaccc agcctcgacg atgcggttgc cgatgaacag ctcgttgaac ccggcatagc
      121 cgtcggcatg gatcacgccg cggaagtcct tcaggtgagc ccgcggatgc tcgcccttgc
      181 gatccggcga gtagaagaac agggccaccg ggggtcgttg gccaccgaat ggccgctcgt
      241 cgcgcacgta agtccataat cggccggtct tggtcttgcc ggcgccagga gccagcactg
      301 gcaccggcgt atcgtcgacg tgcagggtct ccgaggtcat gacgtcgctg gcgagcgcgt
      361 cgatcagcgg ctgcagcgcc gccgccgtcg caccgaccca gcccgacaag gtcgaggtct
      421 ccagttcgac gtcctcgcgg gcgaagatct cggcttggcg gtaaagcggc aagtgatcgt
      481 cgtacttcga gacgacgatg tgcgccagga gacccgcccc ggcgcggccg cgggcgatcg
      541 catgatcggg cgccggcgcg gcgacaaccg tgtcgcaggt gcggcacgac agcttctctc
      601 ggatgtgccg gatcactttg aagcgacccg gcacgtaatc gagcgtctcg gtgatgtcct
      661 caccgatccg gcgcagcgcg tcgccgcagc tcgggcaggc gcacggcgcg gcgtggatct
      721 ggtcctcgcg cggcagatgg tcgggcagcg cgcgccgcgc tggcttcttg ttctcgaccg
      781 ctgcttcgat cgccgcggcg acaatcggcg aggcggcggc aagacgttcg gcctggtcgg
      841 cttccagcgt ctcgatcgcc agctcgagtt ggtcgacctc gcgctgcagc ttctccgacg
      901 agcggccgaa ctcggcccgc cgataacgtg ccagctgaaa gcgcagctta gcgatctcgg
      961 cctgggcgtt gaccgcatcc tgtgccatcg cggcgatgat ccgcttaagc gcatccaggt
     1021 catcgggaag ctgatcgagc gggatcggca cccccgcatt gaatcacgat cgagcagtcc
     1081 ttggcgcgtc taaaaagctc gttgattcaa tcagtcgcac atcatcgcgc gacctgcgga
     1141 cgccacgttc ggcgcggcat tcgccaatca atcccttcga gcagcatgcc cagctgtgcc
     1201 ggcgtgatca ccaccttgcc atcagccggt gacggccata cgaagcgacc tttctccagt
     1261 cgcttggcga acagacatag tccctggccg tcccaccaca gcaccttcag caggtcacca
     1321 cgacggccgc gaaagacgaa caagtggccg cagaacgggt cctgcgccat tcgctcctga
     1381 accgcggcag ccagcccgtc aaaccctttg cgcatatccg tgcggccgac tgccaaccac
     1441 actcgtgcgc ccgacggcaa cccgatcatc gatcgagcac cttgatcacc cgggccagtg
     1501 cacttgccct cacggtgccg tccacgatga cgcggcggcc gttgccgagc acgatctcca
     1561 tccggctggg aaccaactct ggtgcaagcg gcgctagtga cattggcgcg gaggcgacaa
     1621 cgaccgggac gaactcctcg ccgctatcca tcaccagacg accctcgcgc gccgccttgc
     1681 gccaggcaaa gagctggctc gggtgaagac cgtggcgctg cgcaaccgcg ctgacgctcg
     1741 cccccggctg cagcgtctca gcaacaaggt cttgcttcgc cgacaggctc caccgccggc
     1801 gacgctcaac cgtgatgatc tccgatcgct gcatcgtcct agaagttcgc ctagcgctac
     1861 tcctaggaga aagccttcca gccgcccata caaggcgccc tcaccgagcg cttacccccg
     1921 tgctgcgagc gctgttcgaa tgttcgaatg gggccagcgt cacacgaaat accgggcgat
     1981 cgccacgtag atcgccgcgt ctgcgctgcc atttctgatt ggatcagtta aaccccgctg
     2041 ctgccgtgca taacgctaca gcctgaatac ttgcgttgat tcagccctcg cgcgggtgta
     2101 gagcttcgta gcgttgtccc acagtagctt gcgtttgcgc ggctccggca ttgcccaccc
     2161 ggttacgatc tcggccgatt ttgggaaatg gctttcgccg tgcgggtaat cggaggcgta
     2221 catcaggacg tcatcgccga gtaggtcggc gacagcgttg gtgagcgccg cgccttccgg
     2281 gatctcgatg ctctggaaat agcgcccgct ggtgacgtaa tcactgggcc gctggcgcaa
     2341 ctcaggtaag gccgccttga tcgtctcggc gtgctcgtcg agccgcttga tccagaacgg
     2401 cagccaacca tggccggcct cgagcgttcc gatgcgcagg cccggatagc ggtccatcac
     2461 cccgctgccg atcagcgagg ccatgttccg catgccgcac cacggatgcg ccgccgaacg
     2521 ctgcagccag agattgtccc aggtatccag cccgccgggc gcatagggtg gcatcaccgt
     2581 aaaggtgtgc agcaccacgg ccaggtcgag ttcggccgct aaggcataga agggttcgag
     2641 gctcggatgg tcgagaggca ctccaaccgg agcgtagacc atcactcccc aagcccagcg
     2701 cgacttgccc cagtgatgca tttccgcgag cgacccgttg atatcgcggg cgctcgccag
     2761 aacgatcccg ccaagtcttt gtgggaacgc gccacagtaa tcgctcatcc agcggtgata
     2821 agcgcgatac atcgcacttt cgagggtgat gtcatcgcct gacgtaaacg tgccgaacca
     2881 gcccgagggc agggtcagat tgacgtcgac cccttcgtaa tccatgtcgg cgatgcgagc
     2941 ggccgggtcg gcatcaaccc tgggcgacgg ctctctttta cgcgcgacgc cggtaaagcc
     3001 ggccatgtag cccgccggcg cttcgtcagg cttcgcgt
//
LOCUS       DATGEM010000509         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1289522, whole genome shotgun sequence.
ACCESSION   DATGEM010000509 DATGEM010000000
VERSION     DATGEM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289522"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VKQ27_15730"
     CDS             complement(<1..770)
                     /locus_tag="VKQ27_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90432.1"
                     /translation="MSITERALTIVRDHHATRSKRSLGLTVQRWTGVERDDQSCAHQL
                     TADCPVGRLPSALSPGHGAAAPGSTPPAINSIGPKFSGRSRIGYYLRNVPRSMHIRFG
                     MANALAGLLPDYVSGGVRTRLYRLAGLRIGPATFVMGNIRISGTGVSPSDGLAIGARC
                     LISTDVTLNLDAKLTVGDDVTLSPHVLVYTGTHRRGPSSRRCSSEPFGLPVTIGNGCW
                     VRVGAIILPGTTLGAGCVVGAGAVVAGQIPPNSFVEGNP"
     gene            complement(793..1914)
                     /locus_tag="VKQ27_15735"
     CDS             complement(793..1914)
                     /locus_tag="VKQ27_15735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY90433.1"
                     /translation="MLLTTSAVIGGMERVTLGLARALALRCWDVQTVFAPPTDRREFS
                     AWCEAQGVVPAFDRAVRQVVEPHRLTDVAALQHLVRMSKPEVVSIHYGGGHIPIKDVL
                     AVRLATRQRCFISVHSTVPWSVAGERKRTLTRLAAALVSGVICPSRAIRQIVIEAGVP
                     RRKVHVVYNGIATPAPLPTREESRAQLAIPRDRFVIGAVSRLVPEKGMDDLVEAVGRL
                     AAASSDVQLLIAGDGPERARLEAFARGRIGARAIFLGALADPGLVYAASDVCARSSHV
                     EGFGIVFLEAALFGVPGIGTRVGGIPEAIVDGRTGVLVRPRAPAELASAIERLRNDGR
                     LRHRLGAAARERVLAEFTDEGMARGYERLFCSAGAAAPR"
     gene            complement(1944..2675)
                     /locus_tag="VKQ27_15740"
     CDS             complement(1944..2675)
                     /locus_tag="VKQ27_15740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLY90434.1"
                     /translation="MTVYDIGANVGYLTLVFSSLVGPSGHVAAFEPDPRTRRLLQQNI
                     CNNGAKNVRLYPYVVSDRNGIVNFATFEYSLVNHIATERTPADATVLSVQATSLDELV
                     YGCNLLAPSVIKVDVEGAEERVLRGSSRILQRIKPVVIVETRSESYDSIKAMADMHGY
                     SVAVLSKADMELPGQVADVLLVPACRRLAWLTNELVHRCATDVAQPQEWRANLQRNEA
                     RESRRAARGVLHRQRSAAVKAQSVI"
     gene            3413..5170
                     /locus_tag="VKQ27_15745"
     CDS             3413..5170
                     /locus_tag="VKQ27_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90435.1"
                     /translation="MLAKLREDAVGKWAEPLSPAGSIRRRFLRRREFLKLGGAAALTR
                     LHGRIRGMPASSAGWLPAGQGAVPSQVAATGSHKLTYHFDAARTGYVPGSRGPSTADL
                     AKWHKYQTVNLGSAVRGAPLYLDAWQVQKGSAQGQTHNLLYVVASDNTVAAFDTDQLR
                     SASTPLWSKRLGTAYSGDSGANIPGPNGIDGTPVLDPDRRRLYIISQQAAGGGNAAYQ
                     VYALDVDSGDVVSNAPLQFAGGNGRPSFDPNVEDQRTALALAAGRLYGTFAAFLHDDR
                     GLYRGWIVSINPDNLSDQRWFPTILRTRGGGVWGQGGVAFAPDNTFYFATGNAFAIGN
                     EGVSLGFAGEDDYWNKLGDLKPADYGDFLQAIVRMGTGQQDLQVLDWYSPSNARDQNA
                     NDRDLGTSPMLLPEIGGKELLITTGKDGSGYVLDRLHLGGWGGELQKVGVSNDWSMTA
                     PTYFRSAAAEHYVILGGAGKPGLQGFKVVATGDGVVLVNAWAMGDGNVPFGLFPGMPE
                     LAGADDTATLWIADGADDVSQPTLYAVNAIDGKQVWSSAQSPADALPRTPHFPVVTYA
                     ARSIYVGTMTGFVCYGVTG"
BASE COUNT          927 a         1677 c         1661 g          947 t
ORIGIN      
        1 ggatttcctt cgacgaagct gttcggcgga atctgcccgg ccacgaccgc cccggcgccg
       61 accacgcagc cggcacccag cgtcgtgcct ggcaggatga tggcgcccac gcgtacccag
      121 cagccgttgc caatcgttac cggtaggccg aacggttcgg aggagcagcg gcgcgagctg
      181 ggaccccgcc ggtgcgtgcc ggtgtacacc agcacgtgcg ggctgagggt cacatcatca
      241 cccacggtca gcttcgcatc cagattaagc gtgacatcgg tactgatcag acagcgagcg
      301 ccaatcgcca gcccatctga cgggctcacg ccggtgcccg agattctgat gttacccatg
      361 acgaaggtgg cggggccgat gcgcagcccg gcgagccggt agagccgggt ccgcacaccg
      421 ccgctgacgt agtcaggcaa gagcccagca agcgcattcg ccatcccgaa ccgtatgtgc
      481 atggagcgcg gaacgttccg cagatagtag ccgatgcgtg acctaccgct gaacttcgga
      541 ccgatgctgt ttatcgcggg cggtgtcgac cccggcgcgg cagcaccgtg gccgggcgaa
      601 agtgcggatg gcagcctacc gaccgggcag tctgccgtca gttgatgcgc acaggactga
      661 tcatcgcgtt cgacaccagt ccatcgttgc accgtcagtc caaggctgcg cttgctccgc
      721 gtagcgtggt gatcccgtac gatcgtcagc gctcgctcgg taatgctcat gcgttcgctc
      781 ccgtgaggta gatcaccgcg gcgcggcggc cccagcgctg caaaacaggc gctcatagcc
      841 gcgcgccatc ccctcgtcgg tgaattcggc gaggacgcgc tcgcgggcgg cggcgccgag
      901 gcggtgccgg agccggccat cgttgcgcag ccgctcgatc gcgctggcaa gctcggccgg
      961 tgcacgcggt ctgacgagca cgccggttcg gccgtccacg atcgcttccg gaatcccgcc
     1021 aacccgcgtg ccaatgccgg gaacaccgaa aagcgctgcc tcaaggaaga cgatgccgaa
     1081 gccctcgacg tgcgacgatc gtgcacacac atcagaggcc gcatagacca ggccggggtc
     1141 ggcgagcgcg ccgaggaaga tagcccgtgc gccaattcgc ccgcgggcaa acgcctccag
     1201 cctggctcgc tcggggccat ccccggcgat gaggagctga acatcactcg atgcggcggc
     1261 aagcctgccc acggcctcga ccaggtcgtc cattcctttt tctggaacga ggcgggaaac
     1321 ggcgccaatc acaaatcggt cgcggggaat cgcgagctgc gcacggctct cttcacgtgt
     1381 cggcaggggc gcgggcgtcg cgatgccgtt atacacgacg tgcaccttgc ggcgagggac
     1441 acccgcttcg atcacgatct gccggatcgc ccgggacggg cagatcacgc cactgaccag
     1501 cgccgccgcg agcctggtga gcgtgcgctt acgctcgcct gcgaccgacc acggcactgt
     1561 gctgtgcacg ctgatgaagc aacgctgccg ggtcgccagg cgcacggcca ggacatcctt
     1621 gattgggatg tggccgccgc cgtaatggat gctcaccacc tccggctttg acatccgcac
     1681 gaggtgttgc aaggctgcaa catcagtcag ccggtgcggc tccacgacct gccgcaccgc
     1741 acggtcgaac gctggaacca caccctgcgc ctcgcaccaa gccgagaact cgcgcctgtc
     1801 ggttgggggg gcaaagaccg tctggacatc ccagcaccgc agtgcaagcg cgcgggcaag
     1861 tccgagtgtc actcgctcca tcccgccgat aacggcgctg gtggtcagca gcaaagcttt
     1921 acgccggtga gctgtctcag tcatcagatg acgctttgtg ccttcacggc cgctgatctc
     1981 tgtcggtgca agacgccgcg agccgccctt cgcgattcgc gtgcttcgtt tctctgcaga
     2041 ttggcgcgcc actcttgtgg ttgtgcgaca tcagtggcgc aacgatggac gagctcgttg
     2101 gtcagccagg ccaggcgccg gcatgcggga accagcaaca cgtctgccac ttgtccgggt
     2161 agttccatat cggccttgct gagcacggcc acactgtagc cgtgcatatc tgccatcgcc
     2221 ttgatcgagt cataggactc cgaccgcgtc tcaacaatga ctactggctt tattctttgc
     2281 agaatccgag atgatccgcg caaaacacgc tcttctgcac cttcaacatc gacttttatg
     2341 acacttggtg cgagtaagtt gcatccatat acgagttcgt caaggctggt tgcctgtaca
     2401 ctcagtacag ttgcatcggc cggagttcgt tcagtagcta tgtgattcac tagagagtac
     2461 tcaaaggtgg caaaattcac tatcccattg cgatccgaga ccacataggg gtatagtctg
     2521 acattctttg cgccgttatt gcatatgttc tgctgcagca accgcctggt gcgcgggtcc
     2581 ggttcaaacg cagccacgtg cccacttggt ccgacaaggc tcgaaaacac cagggtcaga
     2641 tatccgacat tggcgccaat gtcgtacacg gtcatgcccc gccttgtata ctgccgtaag
     2701 acccgcacga cgtgtcgctc gtaggtgcca agagccaacg ctggatggtc gtccgtaggc
     2761 atgaccaggc gaacaccgcg cagcgggccc caggcgattc gcgatgacat tgcggggccg
     2821 cccgaggccc acaagagtgc ccgcaccagc ccccgtgctg ccatccgacg cgttcggcgt
     2881 gctgctaacc gcgcttgtac atcgagtaca gccatcgatc acctcgatcc ggatagctga
     2941 agccacggcc cgcgcacgcc caaaccgtgg ttcaatgcca agcgtctgtc cgcggcagct
     3001 actctgcgac gatcggacgg cgcctgccgc tctgccgcca cctgcggcgc cgagagttac
     3061 ggaagccagc gccgccgcag ctcttcggag gttgccgatc gatccccgac ccggtggaag
     3121 gggctcgcgc ggcgcgagcc gcttggcctg ttcagcgcca ctccttgagc tggcgccggc
     3181 tgcgactgcg catcgctgtc ggtatcgctc gtcgagcgac tcactgcccg gaccccggag
     3241 agacctgtcg caaacttcgg cgcgtggccc gacttggtac cctagctgag tctcgctgac
     3301 cgcagcgtgt cttcacgtct tggtcctgtg cgaaaccggt ggagatgccg ttgcctgcgc
     3361 cggcggcggt tgagccgctc cgatccactt gctatcttcg aggtgcgccg tcttgctggc
     3421 gaagctccgg gaggatgccg tggggaagtg ggcagaaccg ctgtcgccgg ccggatccat
     3481 acgcaggcgg tttttgcgcc ggcgtgaatt cctcaagctg ggcggtgcgg ccgcgctcac
     3541 ccgcctgcac ggccggatcc gcgggatgcc ggcaagttcc gcaggctggc tgcctgccgg
     3601 acagggcgcc gttcccagcc aggtggcggc gacgggctca cataagctga cgtaccactt
     3661 cgacgccgca cgcacagggt atgtcccagg atcgcgcggt ccgagcacgg ctgatctggc
     3721 gaagtggcat aagtatcaaa ccgtaaatct cggatctgcg gtgcgtggtg cgccgctcta
     3781 cctcgacgcc tggcaggtgc agaaaggatc agctcagggc caaacgcaca atctgctgta
     3841 cgtcgtcgct tcagacaata cggtggcggc ctttgatacg gaccaattgc gttcggcctc
     3901 aacgccgttg tggtcgaagc gacttggtac ggcctattcg ggcgactctg gagctaacat
     3961 tcctgggccg aacggtattg acggcacgcc ggtgctcgat cccgatcggc ggcgtctcta
     4021 catcatctcc cagcaggccg ccggtggcgg caatgccgct tatcaagtct acgcgcttga
     4081 cgtcgacagc ggcgatgtgg tctcgaacgc cccgctgcag ttcgccggtg gcaacggccg
     4141 tccgtcgttt gacccgaacg tggaggatca gcgcacggcg ctggcgctcg ctgccggccg
     4201 tctctacggc acgtttgccg ccttcctgca cgatgaccgc ggcttgtacc gtggctggat
     4261 cgtgagcatt aatcccgaca acctgagcga tcagcgctgg tttcccacca tccttcgcac
     4321 ccgtggcggc ggggtatggg ggcagggtgg cgtggccttc gcaccggaca acaccttcta
     4381 ctttgcaacg ggcaatgcgt tcgccatcgg caacgagggg gtctcactgg gtttcgccgg
     4441 tgaggacgac tactggaaca agctgggcga tctgaagccg gccgactacg gcgactttct
     4501 gcaagcgatc gtgcgcatgg gtaccggcca acaagatttg caggtgctgg attggtacag
     4561 tcctagcaat gcacgtgatc agaatgcgaa tgatcgcgac ctcggcacga gtccgatgct
     4621 gctgcctgag atcggcggca aggagttgct gatcaccaca gggaaggacg gcagcggcta
     4681 tgtgctcgat cggcttcatc tcggtggctg gggcggcgag ttgcagaagg tcggcgtcag
     4741 caatgactgg agtatgacag cgccgacgta ttttcgttcg gcggccgccg agcactacgt
     4801 cattctgggc ggggccggaa agccggggtt gcagggcttc aaagtcgtgg caacgggcga
     4861 tggtgtggtc ctcgtcaacg cctgggccat gggggatggc aatgtcccgt ttggtctctt
     4921 tccggggatg ccggaacttg ccggcgccga cgacacggcg acgctctgga ttgctgacgg
     4981 cgccgatgat gtcagccagc cgacgcttta cgccgtgaac gcgatcgacg gcaaacaggt
     5041 gtggagctcg gcgcagtcgc cggcagatgc cctgccgcgc accccgcact tcccggtggt
     5101 gacgtacgcc gcgcgcagca tttatgtggg caccatgacc ggttttgtct gctacggcgt
     5161 gactgggtag gagcaacctc agcgggcgag gaactgttgc aggcagtcat ca
//
LOCUS       DATGEM010000510         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_753595, whole genome shotgun sequence.
ACCESSION   DATGEM010000510 DATGEM010000000
VERSION     DATGEM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753595"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VKQ27_15750"
     CDS             <1..399
                     /locus_tag="VKQ27_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90436.1"
                     /translation="VPHLMMALVATGRDAAAARFVAALRDLAADPSVWTAPAIADVVI
                     PVCEAAQAHRHGEYARVVDLLASRQDQIRLLGGSNAQRDMFFQMLIDAAMKANRRDVV
                     APMIARETATRAVPPTQRAGYAPAASWLHS"
     gene            464..799
                     /locus_tag="VKQ27_15755"
     CDS             464..799
                     /locus_tag="VKQ27_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HLY90437.1"
                     /translation="MSEEPGDENQPPSFEERLKAARGRQGLDAPPSASAGDTGGADGM
                     SPWGIGLRVGIELVSALVVAVAIGWGLGYLFGGKPIFIAVFVLLGGAAGILNVWRLFA
                     PRPTRNRGR"
     gene            800..1549
                     /locus_tag="VKQ27_15760"
     CDS             800..1549
                     /locus_tag="VKQ27_15760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLY90438.1"
                     /translation="MAAGGIDALSQFQLHPALGAIGASVNFTQSNLMMVVAGVLTFGL
                     LWLGMRPRAMVPGRLQSAAEMAYETVLGMCTETIGPEGRAYFPFVFTLFFFILFGNLL
                     GVFPYFFTFTSHIAVTLALTVFVVGLVTFVAIWIHGWHFFSYFVPQGVPVVLLPLLVP
                     IEILSYVSRLISLSVRLFANMMAGHVMLDVFGAFVIMLGGAGIIFWLPAGFSLAVNVA
                     LIGFELLVATLQAYVFAVLTCIYLHDAVHLH"
     gene            1568..1792
                     /locus_tag="VKQ27_15765"
     CDS             1568..1792
                     /locus_tag="VKQ27_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C family protein"
                     /protein_id="HLY90439.1"
                     /translation="MDLASAKAIGAGIAVIALSGVGVGIGYIFAALVSSVARNPAARE
                     RVFGLAILGFALTEAVALYALVIAFIILFV"
     gene            1792..2358
                     /locus_tag="VKQ27_15770"
     CDS             1792..2358
                     /locus_tag="VKQ27_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HLY90440.1"
                     /translation="MRLLRLALLGLLLPAAAHAEGMPQLDFANPLTTSQVVWGAIIFA
                     VLYIALSRFALPKVGEVIEQRARHIAADLETAQAAKTRSDEAAKQVADATAKARAEAQ
                     AAINAALDVAKQESASRIAALNERLEKQLHDAEAQINAARAAAMGALREVATDTASTV
                     VTRLTGAPPDQGRLSGAIGAALASRQIG"
     gene            2369..2881
                     /locus_tag="VKQ27_15775"
     CDS             2369..2881
                     /locus_tag="VKQ27_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLY90441.1"
                     /translation="MEEPGFFAEPRNWVLIAFIIFFLLFGKKLWGALAGMLDDRAAKV
                     RTELEEAARLRQEAEAMLRDAEKRREEALREAQALIAGAKTEAERVAAAASAEAEASA
                     KRREQMAMDRIAAAEKAAVDEVRTTAAEVATAAARQVIAEGLSAEADAHLIDHAITQL
                     PSALASRRAA"
BASE COUNT          453 a          990 c          961 g          480 t
ORIGIN      
        1 gtgccgcacc tgatgatggc actggtggcg accggtcgcg acgcagccgc cgcccgcttc
       61 gttgccgcgc tgcgggatct ggcagccgac ccttcggttt ggaccgcgcc ggcgatcgcc
      121 gatgtggtga tcccggtctg cgaggccgca caggcgcatc ggcacggcga gtacgcccgg
      181 gtggtcgatc tgctggcgtc gcgccaggac cagatccgcc tgctcggcgg cagcaacgcg
      241 cagcgcgaca tgttcttcca gatgctgatc gatgccgcaa tgaaggcgaa ccgccgcgat
      301 gtcgtcgcac cgatgatcgc gcgcgaaaca gcgacgcggg ccgtgccgcc aacgcaacgc
      361 gccggctacg ccccagccgc gagctggttg cattcttgac actgtacaga cccccctgta
      421 gcaagccccc gccttccggc ggggcttcct gccaggagcg gacatgagcg aggaaccggg
      481 cgacgaaaac cagcctccct ccttcgagga gaggctgaaa gccgcccgcg gcaggcaggg
      541 gctcgatgcc ccgccatccg cgtcagccgg ggataccggc ggcgcggacg gcatgagccc
      601 ttggggcatc ggcctgcggg tggggatcga actggtctcg gccctggtcg tggcagtcgc
      661 catcggctgg gggttgggtt acctgttcgg cggcaagccc atcttcattg cggtgttcgt
      721 cttgctgggc ggcgcggcgg gaatactcaa tgtctggcgc ctgttcgcgc cacgaccgac
      781 acggaacagg ggacgataag tggcggccgg cggaatcgac gcactgagcc agtttcaact
      841 gcaccccgcg ctgggagcca tcggcgcgtc ggtgaacttc acccagtcca acctcatgat
      901 ggtggtggcc ggcgtcctca ccttcggcct gctgtggctg ggcatgcgac cccgcgccat
      961 ggtgccaggc cggctgcagt cggctgccga aatggcctac gaaaccgtcc tcggcatgtg
     1021 taccgagacg atcggccccg aaggccgcgc ctacttcccg ttcgtcttca cgctgttctt
     1081 cttcatcctg ttcggcaacc tgctgggcgt cttcccctac ttcttcacct tcaccagcca
     1141 catcgcggtg acgctggcac tgaccgtgtt cgtcgtcggc ctggtgactt tcgtcgccat
     1201 ctggatccat ggctggcact tcttcagcta tttcgtaccc cagggcgtgc cggtcgtgct
     1261 gctgccgcta ttggtgccaa tcgaaatcct gtcctacgtg tcgcgcctga tctcgctgtc
     1321 cgtgcgcctt ttcgccaaca tgatggccgg ccacgtgatg ctggatgtgt tcggcgcctt
     1381 cgtgatcatg ctgggcggcg cgggcatcat cttctggctg ccggccggct tctcgctcgc
     1441 ggtgaacgtg gcactgatcg gcttcgaact cctggtggcg acactgcagg cctacgtgtt
     1501 tgccgtgctc acctgcatct atctgcacga cgcggtgcac ctccactaac caaagggaat
     1561 acaggacatg gatctcgctt ccgcaaaggc catcggcgcg ggcatcgccg ttatcgcgct
     1621 ctcgggcgtc ggcgtcggca tcggctacat cttcgccgcc ctggtcagca gcgtggcacg
     1681 caaccctgcg gcgcgcgagc gcgtgttcgg cctcgccatc cttgggttcg cgctgaccga
     1741 agccgtcgca ctctacgctc tggttatcgc gttcatcatt ctgttcgtct gatgcggctg
     1801 ctgcgcctcg cgctgctggg gctgctgctg ccggccgccg cgcacgcgga aggcatgccg
     1861 cagctcgact tcgccaaccc gctgaccacc agccaggtgg tgtggggcgc gatcatcttc
     1921 gccgtgctct acatcgcgct gtcgcgcttc gcgctgccga aggtgggcga ggtgatcgag
     1981 caacgcgccc gccacatagc cgccgatctg gaaacagcgc aggcggcgaa gacgcgctcg
     2041 gatgaagcgg cgaagcaggt ggccgacgcc accgccaagg cgcgcgccga ggcgcaggct
     2101 gcaatcaacg cagcgctgga tgttgccaag caggaatccg cgtcgcgcat cgcggcgctg
     2161 aacgagcgcc tggaaaagca gctgcacgac gccgaggcgc agatcaacgc ggcccgcgcc
     2221 gccgccatgg gcgccctgcg cgaagtggca accgacaccg cgtcgaccgt cgtcacccgg
     2281 ctgaccggcg cgccgcccga ccagggtcgg ctgagcggcg cgatcggcgc ggcgctggcc
     2341 tcgcggcaga tcggctagag agggcgtcat ggaagaaccc ggcttcttcg ccgaaccacg
     2401 caactgggtc ctgatcgcct tcatcatttt cttcctcctg ttcgggaaga agctgtgggg
     2461 agcgctcgcc ggcatgctgg acgaccgcgc cgccaaggtg cgcaccgagt tggaggaagc
     2521 cgcccggctc cgccaggagg ccgaagccat gttgcgcgac gccgaaaagc gccgcgagga
     2581 agccctgcgc gaagcccagg cgctgatcgc gggcgcgaag accgaggccg aacgggtcgc
     2641 cgccgcagcg tctgcagagg ccgaagcgtc agccaagcgt cgcgagcaga tggcaatgga
     2701 ccggatcgcc gccgcagaga aggccgcggt ggacgaggtc cggaccaccg ccgccgaggt
     2761 cgcgaccgcg gccgcgcgcc aggtgatcgc cgagggactg tcagcggaag ccgacgcgca
     2821 cctgatcgac cacgcaatca cccagctgcc gagcgccctg gcaagccgaa gggcggctta
     2881 acaa
//
LOCUS       DATGEM010000511         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1516159, whole genome shotgun sequence.
ACCESSION   DATGEM010000511 DATGEM010000000
VERSION     DATGEM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516159"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..831
                     /locus_tag="VKQ27_15780"
     CDS             4..831
                     /locus_tag="VKQ27_15780"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLY90442.1"
                     /translation="MPDAHVEAPTGGSVVLAAIMLKMGGYGFLRFSLPITPDASRHLD
                     WLMIALSLIAVVYICFVALAQRDMKKLIAYSSIAHMGFVTLGAFIGFQIAAHTGKVAG
                     IQMGLDGSLVQMLSHGLVSGAMFLCVGVMYDRMHSREISAYGGVVNTMPKFASFMVLF
                     AMANAGLPGTSGFVGEFLVILSSFKASVWYAFLAATTLVLGAAYTLWLIKRVIFGEVA
                     NDKVAAMKDLDGREFFVLSALAAAVLLFGLWPAPLLDVMRATTHHLAETLLVSKIVP"
     gene            839..>2163
                     /gene="nuoN"
                     /locus_tag="VKQ27_15785"
     CDS             839..>2163
                     /gene="nuoN"
                     /locus_tag="VKQ27_15785"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HLY90443.1"
                     /translation="MNSFSVVDFNSGLGLARPEIFLGLAACAILMLDLVLDDARRHWT
                     GVLAVISLLATAVLVLVQPVSVKVVALGGLFELDRMAQVLKIVMLLTVGAVFVYSTDY
                     LRRRAILKGEYYVLGLFATLGGMVLISAASLITLYLGLELMSLCLYAMVAFDRESGIA
                     AESAIKYFVLGSMASGTLLYGMSIVYGVTGSLELSTIAAAVHGGFGDNVGLLFGIAFL
                     IVGIGFKLGAVPFHMWIPDVYQGSPTCVTVFIGTASKLAAFALAMRLLPEALAGSQTD
                     WSQMLVVLSVLSIVIGNVVAIAQANLKRMLAYSTISHIGYILLGLLAGTAQGYQAAMF
                     YMISYVLVACGAFGMILLLARQGFEADNLVDFKGLNARSPWFAGMMAILMFSLAGLPP
                     FIGFWAKLGVIQAVLNVDYTWLAVIAVLFSVVGAFYYLRVVKLMYFDDPT"
BASE COUNT          314 a          671 c          689 g          489 t
ORIGIN      
        1 tggttgccgg atgcgcacgt ggaagcgccg accggcggtt cagtggtgct ggcggccatc
       61 atgctgaaga tgggcggcta cggtttcctg cggttctccc tgccgatcac gcccgacgcg
      121 agccggcacc tggattggct catgatcgcc ctgtcgctga tcgccgtggt gtacatctgc
      181 ttcgtggccc tggcgcagcg ggacatgaag aagctgatcg cttactcgtc gatcgcgcac
      241 atgggtttcg tcaccttggg cgccttcatc ggctttcaga tcgcggcgca taccggcaag
      301 gtcgccggca tccagatggg gctggacggg tcgctggtgc agatgctgtc gcacggcctg
      361 gtgtcgggcg ccatgttcct gtgtgtgggc gtcatgtacg accgcatgca cagccgcgaa
      421 atcagcgcct acggcggcgt cgtcaacacc atgccgaaat tcgcgtcgtt catggtgttg
      481 ttcgcgatgg ccaatgccgg gctgccgggc acctcggggt tcgtcggcga gttcctggtc
      541 attctctcct cgttcaaggc cagcgtctgg tacgcctttc tcgccgctac gacgctggtg
      601 ctgggggcgg cgtatacctt gtggctgatc aagcgggtga tcttcggcga ggtcgcgaac
      661 gacaaggtcg cggccatgaa ggatctcgac ggccgcgaat tcttcgtgct ctccgcgctg
      721 gccgcggccg tgctgctgtt cggtttgtgg cccgcgcccc tgctcgacgt catgcgcgcg
      781 acgacccatc acctcgccga gacgctgctg gtctcgaaga tcgtccccta aggctgccat
      841 gaactctttc agcgtcgtcg atttcaactc agggttgggc ctcgcccggc cggagatatt
      901 cctgggtctg gcggcctgcg ccattctcat gctggatctg gtgctcgatg acgcccggcg
      961 tcactggacc ggcgtgctcg cggtgatctc gctgctggcg accgccgtcc tggtcctggt
     1021 gcagccggtc tcggtgaaag tggtggcgct cggcggatta ttcgaactgg atcgcatggc
     1081 gcaggtgctg aagatcgtca tgctgctcac ggtgggagcg gtgttcgtct attccaccga
     1141 ctatctgcgc cgccgcgcca tcctcaaggg cgaatattac gtgctgggcc tgttcgccac
     1201 cctgggcgga atggtgctca tctccgcggc cagtctcatc actctgtatc tgggactgga
     1261 gctgatgtcc ctgtgcctct acgccatggt ggcattcgat cgcgaatccg gcatcgcggc
     1321 ggagtccgcg atcaaatatt tcgtgctcgg ctccatggca tcgggcacgc tgctctatgg
     1381 aatgtccatc gtctacggcg tcacgggaag cctggaactc tcgacgatcg ccgccgccgt
     1441 tcatggcggc ttcggcgaca acgtcggact gctgttcggc atcgcattcc tgatcgtcgg
     1501 catcggattc aagctcggcg ccgtgccgtt ccacatgtgg attcccgacg tgtaccaggg
     1561 gtcgccgacc tgtgtgacgg tgttcatcgg caccgcatcg aagctcgcgg ccttcgctct
     1621 cgccatgcgc cttttgccgg aggcattggc cggctcgcaa accgattgga gccagatgct
     1681 ggtggtcctt tcggtgctgt cgatcgtgat cggcaacgtg gtggccattg cacaggcgaa
     1741 tctgaagcga atgctcgcct attcgaccat ttcgcacatc ggctacatcc tgctgggcct
     1801 cctcgccgga accgcgcagg gctaccaagc cgccatgttc tacatgatca gctacgtgct
     1861 cgtcgcgtgc ggagccttcg gaatgatcct gctgctggcg cgccagggat tcgaggccga
     1921 caacctcgtg gatttcaagg ggctcaatgc acgcagcccc tggttcgcgg gcatgatggc
     1981 gattctcatg ttcagtctcg ccggactgcc gccgttcatc ggattctggg cgaagctcgg
     2041 agtcattcaa gcggtgctca acgtcgacta cacctggctg gcggtgatcg cggtgctgtt
     2101 ctcggtcgtg ggcgcgttct actacctgcg ggtggtcaag ctcatgtact tcgacgatcc
     2161 cac
//
LOCUS       DATGEM010000512         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1333724, whole genome shotgun sequence.
ACCESSION   DATGEM010000512 DATGEM010000000
VERSION     DATGEM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333724"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..1163)
                     /locus_tag="VKQ27_15790"
     CDS             complement(438..1163)
                     /locus_tag="VKQ27_15790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90444.1"
                     /translation="MIERLDIAADHQHLDIAAGTGEPGLSIARLSPKGRVVLTDLAAE
                     MLDIAARRARAQGVANIETKVCSADDLPFDDAMFDSVSVRFGYMFFPDVAKATAEFVR
                     VLKPGGRLCSSVWVKPEENPWTAIAMQAIATEAVVAPPGRDGPNMFRCAAPGYVSALY
                     ERAGLRDIAEWDVDVELVTRSPEQYWEMISEHVSLAVAALQQAGEPARERIRARAIAK
                     VSAFEKDGKVRVPGVARCIVGTK"
     gene            1860..>2414
                     /locus_tag="VKQ27_15795"
     CDS             1860..>2414
                     /locus_tag="VKQ27_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90445.1"
                     /translation="MIGIAAPPILLLTIPIGAIGGAIVGLLTGIVDGLVLGTVSRLGW
                     LGNPDSPHRVRAAVIAALATISGGDLALSILADALQGRWIAPPIVRSQLFQVILIVAG
                     AVAAGLSRRLPPEGSPRIRERRRLTPPQRARRRFWDNVDRWAIAGIRIGLAAGVCAGL
                     IGYALVGFAEAALFDLVLYSAAGAI"
BASE COUNT          358 a          874 c          777 g          405 t
ORIGIN      
        1 cgatggccgc ggcctttgcc acccatgagc gcagccgccg gcaggtgccg gatgccgcga
       61 gccagggcct gcggccggtg atcgcggtgc cgggcatcgc cgtcgcggcg agatctctca
      121 cggactcgcc gcgggactcg ctgcgcgtcg cttggtggat gaccatgaca tcgccttccg
      181 atacgggcag ggaccatgcg tccccgcgag tcctcgtatc attgccttcg cccgctggcc
      241 gcgacaatgg tgtatttacc catctcagcc ggacgcggct acccatctaa cccggcacga
      301 tacgcaacag ggcggcgacg cgttgccagc catcgtcggc tgctgagccc ggatggcacg
      361 gcttcggcct acggctgagt cggccagccc gcgttggagg atcgcgcgtc gtcgcgtggg
      421 ggcgtgccgg tcgcgtccta tttcgttccc acgatgcacc gagccacgcc cggaacccgg
      481 accttgccgt ccttctcgaa cgcgctcacc ttcgctatgg cgcgtgcccg aatccgctcc
      541 cgcgccggtt cgccggcttg ctgaagcgcc gcaactgcga gcgagacgtg ctcgctgatc
      601 atctcccaat actgctcggg cgatcgcgtc acgagttcga cgtcgacgtc ccactctgca
      661 atgtcgcgca gccccgcacg ctcgtacagg gcactgacat atcccggggc agcgcaccga
      721 aacatattcg gcccgtcccg gccgggcggc gccaccaccg cctcggtcgc gattgcctgc
      781 atggcgatcg ccgtccacgg gttttcctcg ggcttgaccc ataccgacga gcagagacgc
      841 ccgcccggct tgagcacgcg cacgaactcg gccgtcgcct tggccacgtc agggaagaac
      901 atgtacccaa aacgcacgga gacgctatcg aacatggcgt cgtcgaacgg caagtcgtcg
      961 gcgctgcaca ccttcgtctc gatattggcg accccttgcg ccctggctcg ccgcgcggct
     1021 atgtccagca tctccgctgc caggtcggtc agcacgacgc gtcccttcgg cgacagtctc
     1081 gcgatgctca atcccggctc acccgtgccc gcggcaatgt cgagatgctg atgatctgca
     1141 gcgatgtcga gacgctcgat catcgctgcg ccgaccgggc ccagctgatc catgatgacc
     1201 gagtcccact tctcccagcc cgcagcgagc ccggcccacg tcgctcgctg aacgtcccgg
     1261 atctcatcgg cgctcgtcat cggccctccc ccaatcgatg catccgccga acaggaaggt
     1321 actcctggcg ggagcgcaca ttgcccgatc tggaacacca acgtggctgt gcccgtcgat
     1381 atgcggagcc acgcatatcg gcggctatcg catgtcggtg ttatcccgtc cgtcgcaacg
     1441 cgtgacggtc atagctgtca ccgcgcagtc cgcgacgccg cgactgtgca agttgtggtg
     1501 accgccgacc agcgagggcg gcgaaagcaa ttgtgctcat catcaagcgt tgccgaaccg
     1561 tccgcaccga gcgctgccgc gatctcccaa taacgaggtc gctaacagcg gcccgcgccc
     1621 ggccatgccc cgcgagcgcc gcgcggtcct cagccgggtc tggatacgat gtcgggagca
     1681 gacagcacag gccggggttg cctgccgcct gctgggctcg gtcaagggag aacgcggacc
     1741 ggagcgacga gatgacgcca aacttgctga cgcgccgacg cgggcctttt tgggctgcgt
     1801 tccgggcctg gatcggggcc ggcctggccg cgggcgctgg cctcggaaca gcgcttggct
     1861 tgatcggcat cgccgccccg cccatcctcc tgctcaccat cccgatcggc gccattggcg
     1921 gcgcgatcgt cggcctgctg accggaatcg tggacggcct ggtgctcggc acagtatcca
     1981 ggctgggctg gctgggcaat ccggacagcc cgcatcgcgt tcgggccgcc gtcattgccg
     2041 ccctagccac catcagcggc ggtgacctgg ccttgtcgat cctggccgac gcgctccagg
     2101 gccgctggat cgcgccgccg atcgttcgca gccagctctt tcaggtgatc ctgatcgtgg
     2161 ccggcgcggt ggccgccggg ctgagccggc ggctgccgcc agaaggttcg ccgcggatcc
     2221 gtgagcgccg ccggctgacg ccgccgcagc gcgccaggcg ccggttctgg gacaacgtgg
     2281 atcgatgggc catcgccggg atccgcatcg gcctcgccgc cggagtatgc gccgggctga
     2341 tcggctacgc gctcgtcggc ttcgccgaag ccgcgctgtt tgatctcgtg ctgtacagcg
     2401 ccgcgggcgc gatc
//
LOCUS       DATGEM010000513         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_328976, whole genome shotgun sequence.
ACCESSION   DATGEM010000513 DATGEM010000000
VERSION     DATGEM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328976"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VKQ27_15800"
     CDS             complement(<1..247)
                     /locus_tag="VKQ27_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY90446.1"
                     /translation="MDSFIFFALLQDGITTGAIYLLLAIGLLITFSVTRVIFVPQGDL
                     LAFGALTAASLQSGHVPGTLWLLDGLSVLALVMTRNWL"
     gene            complement(264..1403)
                     /locus_tag="VKQ27_15805"
     CDS             complement(264..1403)
                     /locus_tag="VKQ27_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90447.1"
                     /translation="MKMRLMAAVAVAGLLVATGAAQAQIKLGVILSISGPGAAMGVGY
                     KGAFALLPDEIAGQKVEYIIRDDATDASTGYAIAQKMISEDHVDAFIGPSLSATDAAV
                     APLANKAKVPMLAMAPYEYDPQKEPYTFNDAQPLSLIVDVIFKYIEKQKVKTIGYIGF
                     SDGWGDQVLAATKTSAASADIKILTDERYARTDTTCEAQALKLISMHPQAIMMGNSAT
                     PATLPVVALRHLGYKGVIYGDHGIVSPAFIKVGGAAVNGVIAATSPVVVADQLTDSNP
                     IKPVAEKFVKDYMAKFPGQGVGPFAGYSYDAFLLLQAAIPQALKAGKPGTEEFRVALR
                     DALEKTHEVVGVSGVYNMSPTNHNGQDSRAAVLVEVKDGAWRAMQ"
     gene            complement(1474..2169)
                     /locus_tag="VKQ27_15810"
     CDS             complement(1474..2169)
                     /locus_tag="VKQ27_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90448.1"
                     /translation="MVLQLSGLAAGYGAKTVISDVTFALEPGQILAFLGHNGAGKTTT
                     LKTVMGLLPPRAGEVLFDGQRVDRLDVAQRVAKGLRLLPEGRGIFPDLNVAENIDVVA
                     ARNVTHEPMFGVADIYRLFPVLDERRATRSGSLSGGQQQMLALGLAILGTPRCLMLDE
                     PSIGLAPNLVERMFQQVHDVCKSHAMTAILVEQNVAAAMKIADRVIIMNNGRIVFDGL
                     PQEARESNFWHYF"
     gene            complement(2169..2933)
                     /locus_tag="VKQ27_15815"
     CDS             complement(2169..2933)
                     /locus_tag="VKQ27_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90449.1"
                     /translation="MTAYLQAENLDHHFGGLRALRECTFTINQGRITCLVGPNGAGKT
                     TIFNAITGFLKPDEGSVTFKGRKLDGLRPQAIVHAGIARTFQNLRLFVDLTALDNVMV
                     GIGSQFGEEPIGAIFRPLHTLRGQRRRREEALATLEHVGLADRARDVVRNLSYGEQKL
                     LTVARVLATGAELLLLDEPASGLSAGALDAVMALLRRLQSEGKTLLVVEHNTRVVQQI
                     ADDVLFLHQGHLMAQGTPEAIISDPALAEIYFGGAL"
     gene            complement(2930..>3136)
                     /locus_tag="VKQ27_15820"
     CDS             complement(2930..>3136)
                     /locus_tag="VKQ27_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY90450.1"
                     /translation="LLTMVVVGGARTLAGSIIGPFLLLALPQVLTLIDIPTTIAASAR
                     QLIYGVLLIAFMLFRPQGLAGERL"
BASE COUNT          577 a          991 c         1008 g          560 t
ORIGIN      
        1 ccagccagtt gcgcgtcatc accagggcca ggacggatag tccatccagc agccacagcg
       61 ttcccggcac atggccggat tgcagtgacg ccgcggtcag cgcgccgaag gcgagcaggt
      121 cgccctgtgg cacgaagatc acccgggtga ccgagaacgt gatcagcagg ccgatcgcca
      181 gcaacagata gatcgcgccg gtggtgatgc cgtcctgcag cagggcgaag aagatgaaag
      241 agtccatgcg gcggaatcag ccgctattgc atggccctcc aagcaccgtc cttgacctcg
      301 accagcaccg cggctcgcga gtcctgaccg ttatggttgg tgggcgacat gttgtagacg
      361 ccgctgacgc cgaccacttc atgggtcttt tccagcgcat cgcgcagcgc gactcggaat
      421 tcctcggtgc ccggcttgcc cgccttcaat gcctgcggaa tagcggcctg caacagcagg
      481 aatgcgtcat agctgtagcc ggcgaacggg ccgacgccct ggccggggaa cttcgccata
      541 tagtccttca cgaatttctc cgcgacgggc ttgatcgggt tggagtccgt caactggtct
      601 gcgaccacca cgggtgatgt cgcggcgatc acgccgttca ccgccgcccc gcctaccttg
      661 atgaatgccg gtgaaacgat gccgtggtcg ccgtagatga cgcccttgta gccgaggtgg
      721 cgcaatgcga cgacgggcag tgtcgcgggc gtggcggaat tgcccatcat aatcgcctgt
      781 ggatgcatgc tgatcagctt cagcgcctgc gcctcacacg tggtatctgt gcgcgcatag
      841 cgctcgtcgg tgaggatctt gatgtcggcc gaggcagccg aggtcttggt ggcagccagg
      901 acctggtcgc cccagccatc ggagaagccg atatagccga tggtcttcac cttctgcttc
      961 tcgatgtact tgaagatcac atccacgatc agtgacagcg gctgggcatc gttgaaggtg
     1021 tagggctcct tctgcgggtc atattcgtag ggtgccatgg caagcatcgg taccttggcc
     1081 ttgttggcga gtggcgcgac cgcggcatcg gtggcggaca gcgacggccc gatgaaggca
     1141 tccacatgat cttccgagat catcttttgc gcgatggcat agccggtgct ggcgtcggtc
     1201 gcatcgtcac gaatgatgta ttcgactttc tgaccggcga tctcgtccgg cagcaacgcg
     1261 aaggccccct tgtagccaac gcccatcgcg gcacctggac cgctgatgga aaggatcacg
     1321 ccgagcttga tctgcgcctg cgctgcgccc gtcgccacaa gcaaacccgc gacggcaacc
     1381 gccgccatca gccgcatttt catgtgagtc tccctagcct ttagccccgc ggcaatctca
     1441 ggcagcggga ttctgcgtgt caagcgctcg cgatcagaaa tagtgccaga agttcgactc
     1501 tcgggcttcc tgcggcagac cgtcgaacac gatccgcccg ttgttcatga tgatcacccg
     1561 atcggcgatc ttcatcgcag cggcgacgtt ctgttccacc aggatagcgg tcattgcgtg
     1621 ggatttgcac acgtcatgga cctgctggaa catgcgttcc accaggttgg gcgcgaggcc
     1681 gatggacggt tcgtccagca tcaggcagcg cggggtgccc agaatcgcca gtcccagcgc
     1741 cagcatttgc tgttgccccc cgctcaggct gcccgaccgc gtggcgcggc gttcgtcgag
     1801 cacgggaaac aggcggtaga tgtcggcgac gccgaacatc ggctcgtgcg tgacattgcg
     1861 ggcggcaacc acatcgatgt tctccgccac attgaggtcg gggaagatgc cacggccttc
     1921 cggcagcagg cgcaggccct tggcgacgcg ctgggcgacg tccaggcgat cgacgcgctg
     1981 gccatcgaac agcacctctc cggcgcgggg tggcagcagg cccatcacgg tcttcagcgt
     2041 ggtggtcttg ccggcgccgt tatggcccag gaaggcgagg atctggccgg gttccagtgc
     2101 gaacgttacg tccgaaatca ccgtcttcgc gccatagccc gcagcgaggc cggacagttg
     2161 cagcaccatc aaagcgctcc gccgaaatag atctccgcga gggcgggatc gctgatgatc
     2221 gcctcgggtg tgccctgggc catcaggtga ccctggtgca gaaacagcac gtcgtcggcg
     2281 atctgctgca cgacacgggt gttgtgctcc accaccagca gggtcttgcc ttccgactgc
     2341 aggcggcgaa gcaaagccat gaccgcatcg agcgctccgg cagaaagacc ggacgccggc
     2401 tcatccagca acagcagttc cgcaccggtg gcgaggacac gggccacggt cagcagcttc
     2461 tgctcgccat aggacaggtt gcgcaccaca tcacgcgcgc gatcggccag gccgacgtgc
     2521 tccagtgtcg ccagcgcttc ctcccggcga cgccgctggc cgcgcagtgt gtgcagcggg
     2581 cggaaaatgg cgccaatggg ttcctcgccg aactggctgc cgatgccgac catcacgttg
     2641 tccagcgcgg tgagatcgac gaacaaccgc aggttctgga acgtgcgcgc aatgccggca
     2701 tgcacgatgg cctgcggacg aaggccgtcc agcttgcggc ccttgaacgt gacactgcct
     2761 tcgtccggct tcaggaaacc ggtgatggcg ttgaagatgg tggtcttgcc ggcgccgttg
     2821 ggtcccacca ggcaggtgat gcggccctgg ttgatggtga acgtgcattc gcgcaaagca
     2881 cgcaggccgc cgaagtgatg gtcgaggttc tcagcctgta gatacgcggt cacaggcgtt
     2941 ctccggccag gccttgcggg cggaacagca tgaacgcgat cagcagcacg ccatagatca
     3001 actggcgcgc ggacgctgcg atcgtcgtgg gaatgtcgat cagcgtcagc acctgtggca
     3061 gggccagcag caggaacggt ccgataatcg agccggcgag cgtgcgtgcg ccgcccacaa
     3121 ccaccatcgt cagcag
//
LOCUS       DATGEM010000514         6870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_943342, whole genome shotgun sequence.
ACCESSION   DATGEM010000514 DATGEM010000000
VERSION     DATGEM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943342"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1735
                     /gene="kdpA"
                     /locus_tag="VKQ27_15825"
     CDS             29..1735
                     /gene="kdpA"
                     /locus_tag="VKQ27_15825"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HLY90451.1"
                     /translation="MTWQGWVQIAVFAALVTVVVKPLGGYIVRNVEGGGRVQRTFSSF
                     ERGLYRLAGVDPSEDQGWVDYALALLCFHIAGIAVLYGLLRLQQLLPLNPQQLEALSP
                     DLALNTAVSFATNTSWQSYGGESTLSYLSQMAGIVVQSFMSGAVGVAVALALVRGFAR
                     RSSRAIGNFWVDITRVTLYVLLPVCVVAALIMIWQGVPQTFNPYVTATTLEGAQQTIA
                     LGPVATQKVIAVMSGDGGGFFNVNSSHPFENPTAFTGLIQMLLIFLVGVALTNAFGRM
                     VGDQRHGWALFAVMAVLFIGGLAAIYASESQANPAVAAPGVEQTSGNMEGKEVRFGIG
                     QSALYATVTTASSDGAVDSMHDSYMPLSGLVMMANMMLDEVIVGGPGSGLFGILLFAI
                     VAVFAAGLMIGRTPEYLGKKIEANEVKMTMLALLCVPAAILGIAAFAAVLPAGTGALN
                     NAGPHGFSELLYTYTSGAATNGSAFAGLSANAPFYNLTLAAAMFAGRFLVIIPVLCIA
                     GTLAAKRSVPITAGTLPTNGMQFIGLMIGTILIVGGLTFFPALALGPAAEQFAIAAGL
                     SY"
     gene            1813..3123
                     /locus_tag="VKQ27_15830"
     CDS             1813..3123
                     /locus_tag="VKQ27_15830"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HLY90452.1"
                     /translation="MNRRLPLLLRAIAYGLRGGFLIRPFVIALALGAAGGVLSSLEEQ
                     VPTLGAWVPMTLFPSRQDPQVAQVILSAIATSIMTVVSIVFAILLMTLTLASTQFSPR
                     ILVSFVRDRGTQWTLGVFLGTFSYCMAALPAARSLPVAFVPVLTVSGAMLLALAAVGW
                     LIFFIHHISQAISVNHIVDRIARETELVIDDLMPRPRSRLPPMNRELPAGEVPILNQR
                     SGYIRFIDVDQLLYLAKSWRIRVHVERQVGQFVPAGVPLLLVSHEDRVDAMRTDELLG
                     AFDIGPTRTLQQDVEFGVIQIIDIALRAISPAVNDPSTAISCIDQLSRILIRWLGRAP
                     PDSLLFDPPHVPRVFVPWISVEGMLDTAFEQIRHYAENDFVVSLRLLRAISDIGGTLR
                     HDDIRAALLARARRVTAGCSERLGEVDVTRLQERLAALEVRLAP"
     gene            complement(3133..3855)
                     /locus_tag="VKQ27_15835"
     CDS             complement(3133..3855)
                     /locus_tag="VKQ27_15835"
                     /inference="COORDINATES: protein motif:HMM:NF016705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLY90453.1"
                     /translation="MSASETVNLAGSPPRPGFASELLARLRGLLPQSLNNTPLLARSA
                     ESAGELVVAHHGAVEIRRTITGWALETCVKGEPEQARETALRRLAGYAAGKNSGHGRL
                     GTVRPLVQTAEAAGRWRVRIGVAGTDIDGALTSARNGRVRVRITEAATIAVIRASGRP
                     GPLAIRQAETAIRDAIAGTRWEVAGSAMLRLHTLPNLLPLLGGFEVAVPVIERPVGSA
                     EPSWSRGAAVKEAATASSLPVR"
     gene            complement(3834..4013)
                     /locus_tag="VKQ27_15840"
     CDS             complement(3834..4013)
                     /locus_tag="VKQ27_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90454.1"
                     /translation="MSNWIIDYHHAWLIRLISLVLLVAVLFAFQLLARHVWQAGSSLV
                     RRLAQGTPLCRHQKL"
     gene            4159..4347
                     /locus_tag="VKQ27_15845"
     CDS             4159..4347
                     /locus_tag="VKQ27_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90455.1"
                     /translation="MEEVQFGPRAGLSDNAADAAREQHTHPYPTPGGVLAKIAGVIAI
                     CLGLALLAQLLIPPPPGL"
     gene            4632..4721
                     /locus_tag="VKQ27_15850"
     CDS             4632..4721
                     /locus_tag="VKQ27_15850"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF011342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HLY90456.1"
                     /translation="MTIDYVLGAIVTVGLFVYLVYALIRPEKF"
     gene            4721..6460
                     /gene="kdpA"
                     /locus_tag="VKQ27_15855"
     CDS             4721..6460
                     /gene="kdpA"
                     /locus_tag="VKQ27_15855"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HLY90457.1"
                     /translation="MTFNGWLQIAIYSVLIVLITKPFGGYITRVFNGERTFLRPVVRW
                     LEVGIYRICGVHEDEEQHWVTYAVAMLAFSFAGFVIMYAIQRLQNVLPLNPAGQSAVS
                     PDLAFDTSVSFLTNTNWQSYVPETTMSYLTQMTALTVHNFVSAATGIVLAIVLIRGFA
                     RRSMQTLGNFWVDLTRCVVYILLPLAIVVGLVLIACGTPQNLSAYTDVNTLDGARQVI
                     AQGPVASQEVIKMLGTNGGGFFNANSAHPFENPSALTNFIQMLLIFSIGAALTNVFGR
                     MVGSQRQGWAIFAVMGVLFLIGVIVCYSAEAQGNPAFTPFHVDQTASALQAGGNMEGK
                     EVRFGLADSALFTTITTDASCGAVNNMHDSLLPLAGMVPMVNIMLGEIIFGGVGSGLY
                     GMLLFVIVAMFVAGLMVGRTPEYLGKKLESKEVKMAMLAILILPLSMLGFTALAAVLP
                     VGTGATNNAGPHGFSEMLYAYVSATGNNGSAFAGLSANVPFWNDTLGLAMFIGRFLMI
                     IPMMAIAGSLVRKKIVPTSAGTFPTDGPLFVGLVVGVIAITGGLIFFPAVSLGPVVEN
                     FSMHAGTLFDTGS"
     gene            6465..>6870
                     /locus_tag="VKQ27_15860"
     CDS             6465..>6870
                     /locus_tag="VKQ27_15860"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189055.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HLY90458.1"
                     /translation="MAIEIRNKMRGSQSSVLDPAILGPALWESVVKLSPRVLIKNPVM
                     FVVEIVAALTTIILARELIQGGPISFTFQIVFWLWLTVLFANFAEAVAEGRGKAQAAT
                     LRKARTETPAKRLASLNAESYQTVPAPELKQGD"
BASE COUNT         1138 a         2243 c         2123 g         1366 t
ORIGIN      
        1 ccgccggcaa cggcccgggc caccagccat gacttggcaa ggctgggtgc agatcgcggt
       61 ctttgccgcg ctggtcaccg tcgtggttaa gccgctcggc ggctacatcg tgcgcaacgt
      121 cgaaggtggc ggccgggtgc agcgcacctt ttcctcattc gagcgtggcc tgtatcgcct
      181 cgccggcgtc gatccatccg aagaccaggg ctgggtcgat tacgcgctgg cgctgctctg
      241 tttccacatt gccggcatcg ccgtgcttta cggtttgctg cggctccagc agcttctccc
      301 gctcaatcca cagcagctgg aggcgctgtc gcccgacctg gcgttgaata ctgcagtcag
      361 cttcgccacc aacacaagct ggcaatctta tggcggcgaa tccaccctca gctacctctc
      421 gcagatggct ggcatcgtcg tgcagtcgtt catgtcgggg gctgttggcg ttgcggtggc
      481 gctcgcgctg gtgcgcggct ttgcccgtcg ctcctccagg gcgatcggca acttctgggt
      541 cgatatcacg cgcgtcacgc tctacgtgct gttgccagtc tgcgtggtgg ctgcgctgat
      601 catgatctgg cagggcgtgc cgcagacctt caacccctat gtcacggcga caacgctcga
      661 gggcgcgcag cagaccatcg ccctgggacc ggtcgccacg cagaaggtca tcgccgtgat
      721 gagcggcgac ggtggcggct tcttcaacgt gaactcgtcg catccgttcg agaatccgac
      781 cgcgtttacc ggcctgatcc agatgttgtt aatcttcttg gttggcgtcg cactgaccaa
      841 cgcgttcggc cgcatggtcg gcgatcagcg ccatggctgg gcactgttcg ccgtcatggc
      901 ggtgctgttt atcggcggcc tggccgcaat ctacgccagc gaatcgcagg ccaatccagc
      961 ggtcgccgca cctggcgtcg agcaaacctc cggcaacatg gaaggcaagg aagtccgctt
     1021 cggcatcggc caatccgcgc tgtacgccac ggtgaccacg gcgtcctccg atggcgcggt
     1081 cgactccatg cacgacagct acatgccgct gtccggcctg gtgatgatgg ccaacatgat
     1141 gctggatgag gtcattgtcg gcggcccggg atccggcctg ttcggcatcc tgctgttcgc
     1201 catcgtcgca gtgttcgcgg cgggcctgat gatcggccga acgcccgaat acctcggcaa
     1261 gaagatcgag gccaacgagg tgaagatgac catgctggcg ctgctgtgcg tgccggccgc
     1321 catcctcggt attgccgcat tcgccgcggt cctgcctgca ggcaccggcg cgctgaacaa
     1381 tgcggggccg catggcttct ccgagctgct ctacacctac acctcgggtg cggcgacgaa
     1441 cggcagtgcc ttcgccgggc tcagcgccaa cgcgcctttc tataatctga cgctggcggc
     1501 tgcgatgttt gccggccgct tcctggtaat catcccggtt ctgtgcattg ccggcacgtt
     1561 ggcagcgaag cgcagcgtgc cgatcaccgc tggaacgtta ccgaccaacg gcatgcagtt
     1621 catcgggctg atgatcggca cgatcctgat cgtgggcggg ctgacgttct ttccggcact
     1681 ggcgcttggt cctgcggcag agcaattcgc gattgcggcg ggtctgagct actgataccc
     1741 gccgtccgct tgctatggat gggtgagtgg ttgctcggct agaatgggcg tttacccaca
     1801 tcacgctcag ccatgaaccg aagactgccc ttgctgctcc gcgccatcgc ctatgggctg
     1861 cgcggtggct ttctcatccg cccttttgtc atcgcgctcg cgctgggtgc cgcgggtggc
     1921 gtgttgtcct cgctcgaaga gcaggtgccg acgcttggcg catgggtgcc catgacgctg
     1981 ttcccatcgc gacaggaccc gcaggttgcg caggtgatcc tgagtgccat cgcgacctcg
     2041 atcatgaccg tggtttccat cgtcttcgcc atcctgctga tgacgctgac cctggcctcg
     2101 acacagttct cgccgcgcat cctggtcagc ttcgtgcgcg accggggaac ccaatggacg
     2161 ctgggcgtgt tccttggcac cttctcctac tgcatggccg ctctgccggc cgcgcgctcg
     2221 ctgcccgttg ccttcgtgcc agtactcacc gtcagtggtg cgatgctcct ggcgctggcg
     2281 gccgtcggct ggctgatctt cttcatccat cacatctccc aggccatcag cgtcaaccac
     2341 atcgtcgatc gcatcgcgcg ggaaaccgag ctcgtcatcg acgacctgat gccaaggccg
     2401 cgatcgcggc tgccgccgat gaaccgcgag cttccggcag gcgaagtgcc catcctgaac
     2461 cagcgctcgg gctatatccg cttcattgac gtcgaccagt tgctctatct ggcgaaatcc
     2521 tggcggatcc gtgtccacgt cgagcgccag gtcggccaat tcgttccggc tggcgtacca
     2581 ctgctgctcg tgtcgcacga ggatcgtgtc gacgcgatgc gcaccgacga attgttgggt
     2641 gcgttcgata tcggaccgac gcgcacgttg cagcaggatg tcgagtttgg cgttatccag
     2701 atcatcgata tcgcgctgcg ggcgatctcg ccggcggtga atgatccaag tactgccatc
     2761 agttgtatcg accagctcag ccgtatcctg atccgctggc tcgggcgcgc accgccagat
     2821 tcgctgctgt tcgatccacc gcatgtgccg cgcgtgttcg taccgtggat cagcgtggaa
     2881 ggcatgctcg acacggcgtt cgagcagatc cgtcactacg cggagaacga tttcgtcgtc
     2941 agcctgcggt tgctgcgggc gatcagcgac attggcggaa ccctgcggca cgacgatatc
     3001 cgtgccgcac ttctggcacg cgcgcgccgg gtgaccgcag ggtgtagcga aaggctcggc
     3061 gaagtcgatg tgacgcggct acaggaacgg ctggccgcac tcgaggtgcg attggcgccc
     3121 tgatttccgg tgtcagcgca ctggcaggct ggatgccgtc gccgcttcct tcaccgcggc
     3181 gccacgagac cagcttggtt cagccgaacc caccggccgc tcgatcaccg gcacggcgac
     3241 ctcgaagccg ccaagaagcg gcagaaggtt ggggagcgta tgaagccgca gcatcgcgct
     3301 gccggcgacc tcccatcgcg tcccggcgat cgcatcgcgg atggctgtct cggcttgccg
     3361 gatggccagc ggccccggcc gtcccgaagc gcggatcacc gcgatcgtcg cggcctccgt
     3421 gatgcgaaca cgcacgcggc cgttgcgtgc cgaggttagc gcaccgtcga tatccgtgcc
     3481 ggcaacgccg atgcgcacgc gccagcggcc ggccgcctcc gcggtctgca ccagcggacg
     3541 gacagtgccc aggcggccgt ggccgctgtt cttgcccgcg gcatagccgg cgaggcgacg
     3601 cagcgccgtc tcgcgggcct gttccggttc gcctttgacg caggtctcga gcgcccaccc
     3661 agtgatggtt cggcggattt ccacagcgcc atggtgcgcg acgacaagct cgccagccga
     3721 ctcggcggaa cgcgcaagca gcggtgtgtt gttgagggat tgtggcagca ggccacgtag
     3781 ccgagccaac agctcgctgg cgaatcccgg gcgcgggggc gagcccgcca ggttcacagt
     3841 ttctgatgcc gacatagtgg tgttccttgt gccaaacgtc gaaccaggct gctgccggct
     3901 tgccagacgt ggcgggcgag tagctggaac gcgaacagga cggcgaccaa cagcaccaaa
     3961 ctgatcagac ggatcagcca ggcgtgatga tagtcgatga tccagttgct catgacgcgg
     4021 cggaatctgt gccgtgccgc atataaaatc gattgggata gcgagcgctc cgtataaaga
     4081 caccgcagcc cgacatggat tttatatgcg cacggtcaat atcaggattg tcccatggcc
     4141 atccgctgga gttgacccgt ggaagaagtc cagttcggcc cgcgtgccgg gctgtccgat
     4201 aacgccgccg acgccgcccg cgagcagcat acccacccct atccgactcc gggcggcgtg
     4261 ttggcgaaaa tcgccggcgt catcgcgatc tgtctcggcc tggcgcttct ggcgcaactg
     4321 ctgatcccgc caccgcccgg cctgtaaggc cacgcggcgg ctgatctgga tcaatgcggc
     4381 cctatcaccc aatccacctg ggtacagcac gtatatagaa tttatatgcc gcacccgtcg
     4441 cggtgacatc gaaactatac gccctgtcac ttagtttagc gtgcagcgca acatgcgggc
     4501 tgcatgcagc ggccgtgttg ctgaccgggg aaaatcaatg ctcgacatcg tctacatcct
     4561 gataggcgcc gtgtttctcg gcggctgcgt tctatacgca atcgcctgcg acaatctgtg
     4621 aggccagtgt catgaccatc gactacgtgc tcggcgcgat cgtgacggtc ggcctctttg
     4681 tctatctggt ctacgccctg atccggccgg agaaattctg atgaccttca atggctggct
     4741 acagatcgcg atctacagcg ttctgatagt cctgatcacc aagccgttcg gcggctacat
     4801 cacgcgcgtg ttcaacggcg agcgcacctt cctgcgcccc gtcgtgcgtt ggctcgaagt
     4861 cggcatctac aggatctgcg gcgtccacga ggacgaggag cagcattggg tgacctatgc
     4921 cgtcgcgatg ctggcattca gcttcgccgg cttcgtcatc atgtatgcca tccagcgcct
     4981 gcagaacgtg ttgccgctga atcccgccgg ccagagtgcc gtctcgcccg atcttgcatt
     5041 cgacacctcg gtcagcttcc tgaccaacac caactggcag tcttacgtgc cagagacgac
     5101 aatgagctac ctcacgcaaa tgacggcgct gacggtgcac aacttcgtct ccgccgcgac
     5161 cggcattgtg ctggctatcg tgctgatccg cggcttcgcc cgccgctcga tgcagacact
     5221 gggcaacttc tgggtcgatc tgacccgctg cgtggtctac atcctgctgc cgctggccat
     5281 cgtcgtcggc ctggtgctga tcgcctgcgg cacgccgcag aacctgtccg cctatacgga
     5341 tgtcaacacg ctggacggcg ccaggcaggt catcgcgcaa ggaccggtgg cgtcccagga
     5401 agtcatcaag atgctgggca ccaatggtgg cgggttcttc aacgccaact cggcgcatcc
     5461 gtttgagaac ccgtcggcgc tgaccaactt catccagatg ctgctgatat tttccattgg
     5521 tgccgcgctg accaacgtgt tcggccgcat ggtgggcagc cagcgccagg gctgggcgat
     5581 cttcgctgtc atgggtgtgc tgttcctgat cggcgtaatc gtctgctact cagccgaggc
     5641 gcagggcaac ccagccttca cgccattcca cgtcgaccag acggccagcg cactgcaggc
     5701 cggcggcaac atggaaggca aggaagtccg tttcggcctc gccgactccg cgctgttcac
     5761 caccatcacc acggacgcat cgtgcggtgc ggtgaacaac atgcacgaca gcctgctgcc
     5821 gctcgccggc atggtgccga tggtcaacat catgctgggc gagatcatct tcggcggtgt
     5881 cggctcgggg ctctacggca tgttgctgtt cgtcatcgtg gcgatgttcg tcgccgggct
     5941 gatggtcggg cgcacgccgg aatatctcgg caagaagctc gaatcgaaag aggtgaagat
     6001 ggcgatgctt gccattctga tcctgccgtt gtcgatgctg ggcttcaccg cacttgcggc
     6061 ggtgctgccg gtcggaaccg gagcgacgaa caacgccggc ccgcacggct tctccgagat
     6121 gctctatgcc tacgtctcgg ccaccggcaa taacggcagc gccttcgccg gcctgtccgc
     6181 caacgtgccg ttctggaacg atacgctggg cctcgcgatg ttcatcggcc gcttcctgat
     6241 gatcatcccg atgatggcga tcgccggttc gctggtgcgc aagaagatcg tgccgacctc
     6301 cgccggcacc ttcccgaccg atgggccgct gttcgtcggc cttgtggtgg gcgtcatcgc
     6361 gatcaccggc ggtctgatct tcttcccagc cgtttccctc gggccggtcg tcgagaactt
     6421 ctcgatgcac gccggcaccc tcttcgacac aggcagctaa tccaatggcc atcgaaatcc
     6481 gtaacaaaat gcgcggctcg caatcctctg tgctggatcc ggctatcctt ggtccggcac
     6541 tctgggagtc cgtcgtcaaa ctcagcccgc gggtgctgat taagaacccg gtgatgttcg
     6601 tggtcgagat cgtcgcagca ctgaccacga tcatcctggc acgcgaactc atccagggtg
     6661 gcccgatcag cttcactttc cagatcgtat tctggctgtg gctgacggtg ctgttcgcca
     6721 acttcgccga agccgtggcc gagggccgcg gcaaggcaca ggcggcaacc ctgcgcaagg
     6781 cgcgcaccga gactccggcc aagcgcctgg catcgctgaa cgccgagtct taccaaacgg
     6841 tgcccgcccc tgaactgaag cagggcgaca
//
LOCUS       DATGEM010000515         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1468579, whole genome shotgun sequence.
ACCESSION   DATGEM010000515 DATGEM010000000
VERSION     DATGEM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468579"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VKQ27_15865"
     CDS             <1..1226
                     /locus_tag="VKQ27_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90459.1"
                     /translation="FSDTGTYLSQAIERYAGWDRPVFYSFFLLPLHLTITTWPAIAVQ
                     ALLTVYTLHLVHRALWPDASPWWLLPIVLLLAVTTSLPWVVAQLMPDVFTGLLVLALA
                     LLIFRPECLSIRERLFLAAFAAFMIAAHLSHVLLAFALLLVLLPTRQWLGAAAPLGAR
                     GHLCAIAPCVLAVIAVMSVNLLAFGRASLSPYGNVFLLARIVYDGPGMNVLRRNCPAS
                     GWRLCDFIDRMPATSDEFLWRPDGPVVLSGGAKRVSLEAGPIVAAALQAEPTTELLAV
                     LSNTADQIGMFETGDGLQPWPATVSPWIERDLPRFEAATYRSALQTNGTLRLPQWLVA
                     LHAAVAAGSVAGLLLAALKCRPLRGFVAATLLALLINAAVTGGLSMPHHRYQSRLMWL
                     PPFVLLLAPPGTRRA"
     gene            1226..2689
                     /locus_tag="VKQ27_15870"
     CDS             1226..2689
                     /locus_tag="VKQ27_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLY90460.1"
                     /translation="MRTQIANVFWSGAEAAVSAVLSFASAFIVARLIGPSEVGIGAAV
                     VAVHVLLWVAVNALFADALVQRSVLDSPTFSSAFFASIAVGCVAALIQAGAGQPLHWL
                     LADHRLITMSLLLAAPLPLVGAAGPVQGLLTRDRAYKALALRTVAGQGLGTLVGAVAA
                     LHGAGAWALVMQQFVISAAGALALLICSPSRPDRRVSWARLRELLHIGLPLTISTLVH
                     HGRYRLFALLIGGTAGAAALGQVHMAFRLVDAVRELAFTAQWRLMLPALSEHQDDLTT
                     LHQATDRCLAWSSLFTFPLCAALAVSIQPLVAILLGPVWQASGITALPLIALAAWLFL
                     AFPAGVAVVARGQPRYTLIANIAGTLATVLGLLLIRPVTPLHAVLVWLGAQIFVSPYV
                     LFTNARVLGTTLFRPLRAGIPMMSASLLAVAAAIILPRMIGEPASPAWLLIMRLTVVI
                     TIVLPVAFRFIGDSDPARSLLPRTTPNTRNAGGSAHP"
     gene            complement(2604..3020)
                     /locus_tag="VKQ27_15875"
     CDS             complement(2604..3020)
                     /locus_tag="VKQ27_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY90461.1"
                     /translation="MQQNDRQSIERIYQLWDAALAAKDVDAAVALYAEDVRLESPLVR
                     HLTGAERGIVQGRDALRDFIRLVFERTPPSRGHHRDAFFTDGRRLIWEYPRETPDGDQ
                     MDFVESMEIKDGLIRRHCVYWGWYGVKVIEQDRYHR"
     gene            3156..3401
                     /locus_tag="VKQ27_15880"
     CDS             3156..3401
                     /locus_tag="VKQ27_15880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HLY90462.1"
                     /translation="MPTYDYDCGRCGPFSESHPMAEFDRPQACPACGDPAPRALTLPA
                     IGAGATEAPSAAATPFRSHPGGCSCCAAPRRFSAEAV"
     gene            complement(3406..>3860)
                     /locus_tag="VKQ27_15885"
     CDS             complement(3406..>3860)
                     /locus_tag="VKQ27_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HLY90463.1"
                     /translation="PVMEGINMVNAPVAARDHGIDVAETVHDRPSEYQTLVRVTVETD
                     QLVRSVAGTLFAGSRPRIVEIKGIRVEADFAPHMLYVTNQDKPGFIGRFGATLAGAGI
                     NIATFHLGRASQGGDAICLVSVDEEVPEDVLSMVRTLPLVVQATSLAF"
BASE COUNT          537 a         1370 c         1147 g          806 t
ORIGIN      
        1 tcttctctga caccggcact tatctttcgc aggcgatcga gcgttatgct ggctgggatc
       61 ggccggtctt ctatagtttt ttcctgctgc ctctccacct gacgatcacc acctggccgg
      121 cgatcgcggt ccaggcattg ctcacggtat atacactcca tctcgttcac cgtgcgctct
      181 ggcctgatgc atctccatgg tggctgctgc ccatcgtgct cttattggcg gtcacgacgt
      241 cgctgccctg ggtcgttgcc cagctgatgc cggatgtgtt caccggattg ctggtgctcg
      301 ctctcgcctt gctgatcttt cgtcccgaat gcctgtccat acgcgagcgc ctgttcctgg
      361 cggcgttcgc tgcattcatg atcgcggccc acctgtcgca tgtgctgctg gcgttcgcac
      421 tcctgcttgt gttgcttccc acgcgacagt ggcttggcgc agccgctccg ctcggtgcac
      481 gcggtcatct ctgcgcgatc gcgccatgcg tgctggcagt catcgccgtg atgtcggtca
      541 accttctggc attcggccgc gcttcactct cgccgtatgg gaacgtgttc ctactcgccc
      601 gcatcgtcta cgacggaccg ggcatgaatg tgctccgccg caactgcccc gccagcggct
      661 ggcgcctgtg cgacttcatc gatcgcatgc cggcaacatc ggatgagttc ctgtggcgcc
      721 ccgacggacc cgtcgtactt tcaggcggcg cgaagcgcgt ctcgctcgag gcaggcccca
      781 ttgtagccgc ggcactccaa gccgaaccca ccacggaact tctcgccgta ctcagcaata
      841 ccgccgatca gatcggcatg ttcgagactg gagacgggct gcagccctgg ccggccaccg
      901 tctctccgtg gatcgagcgc gacttgcctc ggttcgaggc cgccacttat cgttccgcac
      961 tacagacaaa tggcacgctg cgacttccgc aatggctggt tgcgctccac gctgccgttg
     1021 cggcggggag cgtcgccggg ttgctgctgg ccgcgctgaa gtgccgaccg cttcgtggct
     1081 tcgtggcggc gaccctgctc gcgttgctga tcaacgcggc ggtcacgggc ggcctgtcca
     1141 tgccgcatca tcgctatcag agccgcctca tgtggcttcc gccattcgtg ctgctgctgg
     1201 cgccgccagg caccaggcgc gcgtgatgcg gacccagatc gccaatgtgt tctggtccgg
     1261 cgccgaggcg gcggtttccg cagtgctgtc cttcgcctcc gcattcatcg ttgcccgact
     1321 gatcggtccg tcggaagtcg gcatcggtgc agcggtggtc gccgtgcatg tgctgctttg
     1381 ggtcgctgtc aatgcgctgt tcgccgatgc cctggtgcag cgcagcgtgc tcgacagccc
     1441 caccttctcc agcgctttct tcgcttccat cgccgtgggc tgcgtggcgg cgctgatcca
     1501 ggcaggtgcc gggcagccac ttcactggtt gctggccgat catcgtttga tcacgatgtc
     1561 cctgctgctc gctgccccat tgccgctggt aggggctgct gggccggtcc aggggctgct
     1621 cacccgcgac cgcgcctata aggcactcgc gttgcgcact gttgccgggc agggcctcgg
     1681 cacgcttgtg ggtgcagtcg ccgcgctgca cggtgccggt gcctgggccc tggtaatgca
     1741 gcaattcgtt atctcggccg caggagcgct cgccctgctg atctgctccc cgagccgtcc
     1801 cgatcgccgg gtaagctggg ctcgcctgcg cgaactgctc cacatcggcc tgcctctgac
     1861 cataagcact ctggtccacc acggccgtta ccgcctgttc gcgctgctga tcggcggcac
     1921 cgcgggtgcc gcggcgctcg gccaggtgca catggcgttc cgcctcgtcg acgccgtgcg
     1981 cgagcttgcc tttactgcgc aatggcggct gatgcttcct gccctctccg agcatcagga
     2041 cgatctcacc accctgcatc aggcgacgga tcgttgcctc gcatggtcga gcctgttcac
     2101 gtttccgctt tgcgcggcgc tcgccgtctc catccagcct ttggttgcga tcctgctcgg
     2161 gccggtctgg caggcatcgg gcattacggc actgccgctc atcgcccttg ccgcgtggct
     2221 cttcctggcc tttccggccg gagtcgccgt cgtggcacgc ggccagccgc gctacacgct
     2281 gatcgccaac atcgccggca ccttggccac cgttcttggc ctgctcctga tccgccccgt
     2341 gacgccgctg catgccgttc tggtctggct tggagcgcag attttcgtca gcccctacgt
     2401 gctcttcacc aacgcgcgcg tgctcggcac gacgctgttc cggccgctgc gcgccggcat
     2461 tccgatgatg agcgcatcgc tcctagccgt cgccgctgcc atcattcttc cgcgcatgat
     2521 cggtgagccc gcatcaccag catggctcct gatcatgcgg ctcaccgttg tgatcaccat
     2581 cgtgttgccg gtcgcattcc ggttcatcgg tgatagcgat cctgctcgat cacttttacc
     2641 ccgtaccacc cccaatacac gcaatgccgg cggatcagcc catccttgat ctccattgat
     2701 tcgacgaagt ccatctggtc gccgtccggc gtttcacgcg ggtactccca gatcagcctg
     2761 cgtccatcgg tgaagaacgc gtcgcgatga tgtccgcggc tcggtggcgt gcgctcgaac
     2821 accaaccgga tgaaatcgcg cagggcatcc cgtccctgca cgataccccg ctccgctccg
     2881 gtcagatgcc gcaccagagg gctttcgagg cgcacgtcct cggcataaag cgcgaccgcg
     2941 gcatccacat cctttgctgc cagcgccgca tcccagagct ggtagatgcg ctcgatcgac
     3001 tgcctgtcat tctgttgcat gacccatgca ctcctgctgt ggaaggcgga tcatcgcagc
     3061 ccggcactga cagcgttctg tcagcagtca ttggctggac ccgcgctgac cttcagtgcc
     3121 aaaatgccgc gtcggttccc tggcgagagg cgcgcatgcc cacctacgac tacgattgcg
     3181 gccgttgtgg cccattcagc gaaagccacc cgatggcaga attcgaccgt ccgcaggcat
     3241 gcccagcctg cggcgatcca gcaccgcgcg cgctgacgct gccggcgatc ggagccggtg
     3301 ccaccgaggc gccctcggct gccgcgacac cgttccgctc acatccgggc ggatgcagct
     3361 gctgcgccgc gccccgccgc ttctcggccg aggcggtctg aagggtcaga acgccagcga
     3421 cgttgcctgc accacgagcg gcagcgtccg gaccatcgac agcacgtcct ccggcacttc
     3481 ctcatccacc gatacgaggc agatcgcatc gccgccctgg ctggcgcgcc ccaggtggaa
     3541 ggtagcgata ttgatcccgg cgcctgccaa tgttgcgccg aaccgtccga tgaagcctgg
     3601 tttgtcctgg ttggtcacgt acagcatgtg gggcgcaaaa tccgcctcca cacggatgcc
     3661 tttgatctcg acgatgcgcg gccgcgagcc agcgaacagc gtgcccgcca ccgagcgcac
     3721 gagctgatcg gtctctacgg tcacacgcac cagcgtctga tattcgctgg gccgatcatg
     3781 caccgtctcg gcgacatcga tgccgtgatc acgcgcggca accggcgcat tcaccatgtt
     3841 tatgccctcc atcaccgggg
//
LOCUS       DATGEM010000516         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1415150, whole genome shotgun sequence.
ACCESSION   DATGEM010000516 DATGEM010000000
VERSION     DATGEM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415150"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VKQ27_15890"
     CDS             <1..969
                     /locus_tag="VKQ27_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90464.1"
                     /translation="NPDYNWNSPIFSNPGPAYVDGVKIRIIPEDNTRIAEFQAGNVHL
                     VQDVPQGDVDRLSKTPGVSIVRYDQLQTTYVGMNASKPPFDDPRVRQAVSYAINRDEI
                     VTGANFGLAKPATTMLTPETPYFWQDGLQAAPNFDASKAGTLLDQAGWTMGAGGIREK
                     GGQQLVLPLWVINDNTTVLQAQILEQQLAKVGIKVDTKQYEQTAWFAAARSGEQTGFI
                     IGVFFENADVLYFYFYSKQQPAPNRFSYSNPQVDQWLDDSRTNPDKNAVTQDYQNIQK
                     QLIDDAPAAPLVHAVGTLGVADGVQGVKVHASRWLYRLTDIWLSKK"
     gene            995..1936
                     /locus_tag="VKQ27_15895"
     CDS             995..1936
                     /locus_tag="VKQ27_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90465.1"
                     /translation="MTQFILRRLLILPVVLLGVSVVVFMVLHLVPGNPAEVIAGPDAP
                     PETVRQIEVELGLDKPLPEQYALYMGRVLQGDLGRSLRSRRPVLADVLDALPNTIELT
                     LVAAVITPLIAIPLGVVAAARRRSALDSGLMLVSMVGITAPAFATSLGLMWLFGYQLR
                     WFPISGDAGPLWTLDGLRSVFLPALALAIGAVAALARLTRSAMLDVLNQDYVRTARAK
                     GLREQVVLLQHGLRNALLPVVTVIGLQVALLLSGAFVTETVFAWPGIGRLAVFAISSR
                     DFPVVQGTVLVIALLFVLINLVVDVMYAYVDPRIHYS"
     gene            1948..2847
                     /locus_tag="VKQ27_15900"
     CDS             1948..2847
                     /locus_tag="VKQ27_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90466.1"
                     /translation="MATIARPLPTADAAVPHSGAALPAGRPLWRRFVRNRPAVIGLCI
                     IVLMLALAALAEVIAPYDWRAQDIINRFSGPSPQHLLGTDELGRDILSRVLYGARFSL
                     TIGFGAVAISLAIGTLVGLVAGFYRGVDGVIMRVVDIMMAFPGILLAIAIVAALGPGL
                     TSTIVAIGVNEIPGFARLNRSLALSLREREYVMAARVLGAPDRVILGRHVLLNLISPL
                     IVFASLRMATAILVGATLSFLGLGVQPPVPEWGAMVSTARQYIATAPHTFVYPTLAIF
                     ITVVGFNLLGDGLRDALDPTLRD"
     gene            2864..3673
                     /locus_tag="VKQ27_15905"
     CDS             2864..3673
                     /locus_tag="VKQ27_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLY90467.1"
                     /translation="MTAFVLSSWRCALLSGAALGLVGCNSAAPASPTTPPAAAPTVAP
                     ATSPAAKPVVSPSPAASPSAVIKPAASPAASVGPSPSAAASASPAISNAAYVTAILFT
                     GSATSGFTLQTPAFTNGGTIPTMYTCAGANGGTSPQLSWSGAPANTQSLALVEQDADT
                     ATPNTHWLVYNIPPSVTQLDAGQPTETPTLPNGAMQGQDHAQGIFAAFALIGYEGACP
                     PPGAAAHHYTFQLFALDGMVPLKPGATINDLKTAMAGHMVGQTELIGLFSR"
     gene            3705..>3969
                     /locus_tag="VKQ27_15910"
     CDS             3705..>3969
                     /locus_tag="VKQ27_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90468.1"
                     /translation="MIAEATCKRAMPDPLILYAPLLDAELRAIADQLRPSGFEFRNVR
                     PAEIPSAIMDADYLCGFIGPIDTAVLVAAADRRLKLVQLMSAGY"
BASE COUNT          644 a         1310 c         1238 g          777 t
ORIGIN      
        1 aatccggact acaactggaa ctcgccgatt ttcagcaacc cgggtccggc gtatgtcgac
       61 ggcgtgaaaa tccgcatcat tcctgaagac aacacgcgca tcgccgaatt ccaggccggc
      121 aacgtccacc tcgtgcagga tgttccgcaa ggcgacgtcg atcgcctgag caagactccg
      181 ggcgtctcga tcgtgcgcta cgaccagttg cagaccacgt acgtgggtat gaacgcgtcg
      241 aagccgccgt tcgacgatcc gcgggtgcgg caggcggtga gctatgcgat caatcgcgac
      301 gagatcgtca ccggcgccaa ctttgggctc gccaaaccgg cgacgacgat gctcacgccc
      361 gagacgccgt acttctggca ggacggtctc caggcagccc ccaacttcga cgcgagcaag
      421 gccggcacgt tgctggacca ggctggctgg acgatgggcg ccggcgggat ccgtgaaaag
      481 ggcggacagc aactggtgtt gccgctgtgg gtcatcaacg acaacaccac cgtgctgcag
      541 gcccagattc tcgagcagca actggccaag gtgggcatca aagtcgacac caagcagtac
      601 gagcagaccg cgtggttcgc cgccgcccgc tctggtgagc agaccggctt catcatcggc
      661 gtcttcttcg aaaatgccga cgtgctgtac ttctacttct actcgaagca gcagccagcg
      721 cccaatcgct tttcgtacag caacccgcag gtcgaccagt ggctggacga ttcgcgaacc
      781 aatcccgaca agaatgccgt cacccaggac taccagaaca tccagaagca gttgatcgac
      841 gacgcgccgg ctgcgccact ggtccacgcc gttggcacgc tgggcgtcgc cgacggcgtg
      901 cagggcgtga aggtgcatgc ctcgcgctgg ctgtatcgcc tgaccgatat ctggttgtca
      961 aagaagtagg gctcccacac cggtaggagg aagcatgacg cagttcatcc tccgcaggct
     1021 gctgatcctg cccgtggtgc tgcttggcgt ctcggtggtg gtgttcatgg tgctccacct
     1081 ggtgccgggt aaccccgccg aggtgatcgc ggggccggat gctccgcctg aaaccgtgcg
     1141 ccagatcgaa gtggagctcg gactggacaa gcccttgcca gagcagtacg cgctgtacat
     1201 gggccgcgtg ctccagggtg atctcggacg atcgctgcgc agccgccggc cggtgctcgc
     1261 ggacgttctc gatgcattgc ccaacacaat tgagctgacg ctggtggcgg cagtcatcac
     1321 gccgctgatc gctattccgc tcggagtggt ggccgcggcg cgcaggcggt cggcattgga
     1381 cagtggcctg atgctggtgt cgatggttgg catcaccgcg ccagcgttcg caacctcgct
     1441 gggtctgatg tggctgttcg gttaccagct ccgctggttc ccgatcagcg gcgacgccgg
     1501 accactctgg acgctggatg gtctgcgtag cgtgttcttg ccggcgctgg cgctggctat
     1561 cggcgcggtc gccgcgctcg cgcggctcac tcgctcggcg atgctcgacg tactcaacca
     1621 ggattacgtg cgtaccgctc gagccaaggg tttgcgcgag caggtggtcc tgttgcagca
     1681 cggactccgc aatgcgctgc tgcccgtggt gaccgttatt ggactccagg tcgcgttgtt
     1741 gctgagcggc gcgtttgtca ccgagaccgt ctttgcctgg cctggtatcg gccgcctggc
     1801 ggtattcgcg atttcgtcgc gggattttcc cgtcgtgcag ggcaccgtac tggtgatcgc
     1861 gctgctgttc gtgctgatca acctggtggt ggacgtgatg tacgcctacg tggatccgcg
     1921 catccactac tcctgagcct ttgtacgatg gctaccatcg ctcggccact ccccacggcg
     1981 gacgcggcag tccctcacag cggagcggcg ctgccggctg gtcgaccgtt gtggcgccgc
     2041 ttcgttcgga atcggcccgc ggtcattggc ttgtgcatca tcgtgctgat gctcgcactc
     2101 gccgcgctgg cggaagtcat cgcaccgtac gactggcgtg cgcaagacat catcaatcga
     2161 ttcagcggac cttcgccaca gcatctgctc ggcactgatg aactcgggcg cgacatcctg
     2221 tcgcgggtgt tgtacggggc gcgcttctcg ctgacgattg gtttcggcgc ggtggccatc
     2281 agtttggcga tcggtacgct ggttgggctg gttgccggct tctatcgagg tgtggacggc
     2341 gtcatcatgc gggtggtcga catcatgatg gcgtttccag gcattctgct ggcgatcgct
     2401 attgtggcag cgcttgggcc gggactgacc agcactatcg ttgccatagg cgtgaacgag
     2461 attcccgggt ttgcgcggct caatcgctcg cttgcattgt cactccgcga acgtgaatat
     2521 gtgatggctg cgcgagtgct tggcgcgccc gaccgcgtca ttctgggtcg ccacgtgctc
     2581 ctgaatttga tttctccgtt gatcgtgttt gcctcgttgc gcatggccac ggcgatcctg
     2641 gttggcgcca cgctgagctt cctcggactg ggtgtccaac cgccggtgcc cgagtgggga
     2701 gcgatggtga gcactgccag gcagtacatc gccaccgcgc cgcatacgtt tgtctatcca
     2761 acgctggcga tcttcatcac cgtggtgggc ttcaaccttc tcggggacgg gttgcgcgac
     2821 gcgctcgacc ccacattgcg cgattgacca tagaatgcgg cgtgtgaccg ctttcgtcct
     2881 gtcctcatgg cgatgcgcat tgctcagcgg tgcggcgctt ggcctcgtcg gctgcaattc
     2941 cgccgctccg gcctctccga ccaccccgcc cgccgcggcg ccaacggtgg caccagccac
     3001 ctcgccggcc gcgaaaccgg tggtctcacc ctcacccgcg gcctcgccgt ctgccgtgat
     3061 caagccggcg gcatcgccgg cggcctcagt cggcccgtcg ccctcggccg ccgcatcggc
     3121 ttcacctgcc atctcgaacg cggcatacgt gaccgccatt ctcttcaccg gctcggcgac
     3181 cagcggcttc acgctgcaaa cccccgcttt caccaacggc ggcaccattc caacgatgta
     3241 cacgtgcgct ggcgccaacg gtggcaccag tccgcaattg agctggtccg gggcgccggc
     3301 gaacacccaa tcactcgcgc tcgtcgagca ggatgcggat accgccacgc cgaatacgca
     3361 ctggctggtg tacaacattc cgccgagcgt cacgcagctc gacgccgggc agccgaccga
     3421 gacccccacc ttgccgaatg gcgcgatgca gggccaggac cacgcgcagg gcatctttgc
     3481 cgcctttgcg ctgatcggct acgaaggtgc ctgtccgcca cctggagccg cggcgcacca
     3541 ctacaccttc cagctcttcg ccctcgacgg aatggttccg ctgaagcctg gtgcgaccat
     3601 caacgacctc aagaccgcga tggccggcca catggtcggt cagaccgaac tgatcggcct
     3661 gttcagtcgc tgagcccgaa tgccgaatcc agccattccg caaggtgatc gccgaagcga
     3721 catgcaagcg cgccatgcct gatccactca tcctctacgc gccgctgctc gacgcagagc
     3781 tgcgcgcgat cgctgatcag ctgcgtccct ccggattcga gttccgtaac gtgcggcccg
     3841 ctgagattcc ctcggcgatc atggatgccg actacctgtg cggcttcatc ggtccgatcg
     3901 acaccgccgt gcttgtcgcc gcggctgacc gccgcttgaa gctggtgcaa ttgatgtccg
     3961 ccggctacg
//
LOCUS       DATGEM010000517         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1057541, whole genome shotgun sequence.
ACCESSION   DATGEM010000517 DATGEM010000000
VERSION     DATGEM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057541"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VKQ27_15915"
     CDS             complement(<1..467)
                     /locus_tag="VKQ27_15915"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HLY90469.1"
                     /translation="MPDAVHLTELTGDYQCLHEFIPAARAKVDSNIWGYLIGATESET
                     TLRRNRAALDAIALRPRALRNVSKVDAGTTFLGMKMRLPVMLAPVGSLESFNPGGAAT
                     AGRGATEFGVPIIVSSVTKPGLEETAKAVTGPKVFQLYVRGDDSFVDDHVHRAV"
     gene            complement(532..1761)
                     /locus_tag="VKQ27_15920"
     CDS             complement(532..1761)
                     /locus_tag="VKQ27_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90470.1"
                     /translation="MLVSRCAFGLNTLNLFVAAAQTGFGPFLSVYLIEQGWNTADIGF
                     ALSIGTVSVLVLQLPSGALVDLIHQKRFANLIGLVLLGFSAWIMLAVPTQGPVLTAQI
                     MHSVASCILTPAIAALTLVLCGQARFSEQLGVNARYASIGNALTAGLLGLLASYVSNR
                     AVFVATTLLVLPAIAVLPLFRNSDRVPADDHPAVLHPTERLRRQHRPWHIFDEPAFVV
                     FVISAVLFQFSNAAMLQLALSELAKRSGRAGLVVSAAIIVPQIVVAVFSPLVGRLAQS
                     LGRRPVLLVGFLALPVRGLLFMTLPEAVPLVVIQVLDGISGAVFGLMVPLIAADVTRR
                     SGFLNFGINSLSLAGGLGATFSTTAAGWVAYTLGAPVAFFGLALVGVAALLLIWFLLP
                     ETRTGKPITGAPQPVPA"
     gene            complement(1908..2963)
                     /locus_tag="VKQ27_15925"
     CDS             complement(1908..2963)
                     /locus_tag="VKQ27_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY90471.1"
                     /translation="MRIAQISPLFEAVPPKLYGGTERVVYSLTEELVAMGHDVTLFAS
                     GDSITSAKLAPMRDQALRLDPTVVDWIAIYMRMMELIYRRADEFDVLHFHTDYFPLSL
                     FSRQKTPFLTTLHGRLDIPEFKDVYELFDAPMVSISDSQRRPIPRLNWIRTVYHGMPA
                     DLLTPQPVKPGYLAFLGRVSPEKGLDKAIRIAGAAGMKLKVAAKVDNADKKYYENEIK
                     PLIAASPWVEFIGEINDAQKPDFLSGAHALLFPIDWPEPFGLVMIESMACGTPVIAFN
                     RGSVPEVIDDGLSGFIVHDEASAIAAVERLDELDRAKVRATFDRRFTARRMAEDYLDL
                     YTELCEPALPLLRAVGD"
     gene            3161..>3982
                     /locus_tag="VKQ27_15930"
     CDS             3161..>3982
                     /locus_tag="VKQ27_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90472.1"
                     /translation="MTEGFEAPRLRTHPIGFMWHYVRRHPWSHTTVLVSVMVAVACAV
                     SVQYGLKHLVDVISVGPAKAQGLWLAFAILCGLVAADSLLWRVAGWAAAYTFVAVTGD
                     IRRDLFGHLSGHSPSYFSERLPGALASRITATSNAAFTIENTGSWNVVPPVVAVVCSI
                     GFIASVNPTMAGTLVAASAALGMLIFYLAKHGTPLHRSFAAKAAAVDGELVDVVSNMS
                     VVRAFGAMFREQRRIAGTIGVELGARRSSLIYLERLRLIHAVITATLIAGIVGWGL"
BASE COUNT          768 a         1312 c         1230 g          672 t
ORIGIN      
        1 accgcgcgat ggacatgatc gtccacaaac gaatcatccc cgcggacata aagctggaag
       61 accttcggtc ccgtgaccgc cttcgccgtc tcctccagcc ccggcttggt gaccgagctc
      121 acgatgatcg gtacgccgaa ctccgtggcg ccgcgtccag ccgtcgccgc accacccgga
      181 ttgaaggact ccagcgagcc aaccggcgcc agcatcaccg gcaggcgcat cttcatccca
      241 aggaacgtcg tcccggcatc caccttcgac acattgcgca gcgcacgtgg ccgcaacgcg
      301 atcgcgtcca gcgcggctcg attgcggcgc agagttgtct cgctctcggt ggcaccgatg
      361 agatagcccc agatgttgct gtccaccttg gcccgcgcgg ctgggatgaa ttcgtgcagg
      421 cactgatagt cgccggtgag ttcagtcagg tggacggcgt cgggcatctt cctctcccta
      481 tgcggttggc gccatctggc ccgcccagcg ctggaaccgc aagaggcgga ttcaggccgg
      541 cacgggctgc ggcgctccgg tgatcggctt gccggttcgc gtctcaggca gcaggaacca
      601 gatcagcaac agtgcagcga ctcccacgag cgccaggccg aagaatgcca ccggtgcgcc
      661 cagcgtgtag gccacccagc cggccgccgt ggtactgaac gtggcgccca gccctccggc
      721 aagcgacagc gagtttatgc cgaaattcag aaagccgctg cggcgggtca cgtcggcggc
      781 gatcaacggc accatcaggc cgaacaccgc gccgctgatg ccgtcgagca cctggatcac
      841 caccaatggc accgcctccg gcagcgtcat gaacagcagc ccacgtaccg gcaacgccag
      901 gaaacccacc agtagaaccg ggcggcgccc caggctctgc gccagtcggc caaccaacgg
      961 cgagaacacc gcgacaacga tctgcggcac gattatggcg gccgacacga ccagcccggc
     1021 gcggccggag cgctttgcca gctcgctcag ggcaagttgc agcatggcgg cattgctgaa
     1081 ctggaacagc accgcgctga tcacgaacac cacgaaagcc ggctcatcga aaatgtgcca
     1141 cggccggtgc tgccgtcgca ggcgctcggt cggatgcaga acagcggggt ggtcatccgc
     1201 cggcacgcgg tcgctgttgc ggaacagcgg cagcaccgcg atcgccggca agacgagcag
     1261 cgtcgtcgcc acgaaaacgg cgcggttcga cacatacgaa gcgagcaggc ccagcagtcc
     1321 agcagtcagc gcattgccaa tcgaggcata acgggcgttg accccgagct gctcgctgaa
     1381 ccgcgcctgg ccacagagca ccaacgtaag tgccgcgatg gcaggtgtca ggatgcagct
     1441 cgccaccgag tgcatgatct gcgcagtcag gaccggaccc tgcgtcggca ccgccagcat
     1501 gatccaggca ctgaaaccca gcagcaccag gccgatcagg ttggcgaagc gcttctgatg
     1561 gatcaggtcc accagcgccc cggatggcaa ttgcagcacc aggaccgaaa ccgtaccgat
     1621 gctgagggca aagcctatat cggccgtatt ccagccctgc tcgatcagat agaccgacag
     1681 gaacggccca aacccggtct gtgccgccgc aacgaacaaa ttgagcgtgt tgagcccgaa
     1741 ggcgcagcgg gagaccagca tcgatcgaga cgtgccattc ctgaggattg tcggcttgga
     1801 ttaaaccacc ctcgcaggaa tgcgagccgt cattcaaccc ccctgaacgc cgagggccgg
     1861 acagacgatc tgcccggccc ccgtaaaggc gttgcttctg gcgagactta gtcgccgaca
     1921 gcgcgcagca gcggcagcgc cggctcgcac agctcggtgt agaggtcgag gtagtcctcg
     1981 gccatccgcc gcgcagtaaa ccggcgatcg aacgtcgccc gcaccttcgc gcggtccagc
     2041 tcgtccaggc gttcgacagc cgcgatggca ctcgcctcat cgtggacaat gaagccggac
     2101 aggccgtcgt cgatcacctc cggcaccgag ccacgattga acgcgatcac cggcgtgccg
     2161 caggccatgc tctcgatcat caccaggcca aatggctccg gccagtcgat cgggaacagc
     2221 agcgcatgcg cacccgacag aaagtccggc ttctgcgcgt cgttgatctc gccaatgaac
     2281 tcgacccagg gactggcggc aatcagcggc ttgatctcgt tctcatagta tttcttgtcc
     2341 gcattatcca ccttcgcggc gaccttcagc ttcatgcccg ccgcgccggc gatgcggatt
     2401 gccttatcca ggcccttttc tggcgatacg cgtccaagga aagccagata gccgggcttc
     2461 accggctgcg gggtgagcag atccgccggc atgccgtggt acacggtgcg gatccagttc
     2521 aggcgcggga tcggccgccg ctgactgtcg gagatcgaca ccatcggcgc gtcgaacagc
     2581 tcgtagacat ccttgaattc cgggatgtcg agccggccat gcagcgtggt gaggaatggc
     2641 gtcttctggc ggctgaaaag tgacagcggg aagtagtcgg tatggaagtg cagcacatcg
     2701 aactcgtcgg cgcggcgata gatcagctcc atcatgcgca tgtagatggc gatccaatcc
     2761 acaaccgtcg gatcgagacg cagcgcctga tcgcgcatcg gtgcgagctt ggcagatgta
     2821 atcgagtcac cggatgcgaa cagggtcacg tcatgaccca tcgcgaccag ctcttcggtg
     2881 agcgaatata cgacacgttc agtgccgccg tacagcttgg gcggcaccgc ttcgaaaagc
     2941 ggagagatct gagcaatacg catgaatgcg gccccggttt ttctatgtgt tcacaagtaa
     3001 ccagctcggc tttactgaca cttgacttat gcagaggccg cgcttaatca ctatctcgtt
     3061 ataggatggg aatgtggcga aaaatcggcg ggtattccgc cgtaaatcag ccttcggacc
     3121 gggtgaaatg aagacaatta cggcagaaat gaggcgcgat atgacggagg gtttcgaggc
     3181 accacgcctc cgcactcatc cgatcggctt tatgtggcat tacgtgcggc gccatccctg
     3241 gagccacacc acggtgcttg tatcggtgat ggtggcggtg gcctgcgccg tctccgttca
     3301 gtacggcctg aagcatttgg tcgacgtgat ctccgtcggt ccggcgaagg cccagggcct
     3361 gtggctggca tttgccatac tgtgcggact ggtcgctgcc gacagtctgc tttggcgtgt
     3421 tgccggctgg gcggccgcct atacatttgt cgccgtgacc ggcgacatcc ggcgcgacct
     3481 ctttggccac ctctccggcc attcgccgag ctacttctcc gagcgcctcc ctggcgcgct
     3541 ggccagccgc atcaccgcga cctccaacgc cgccttcacc atcgagaaca ccggatcgtg
     3601 gaacgtggtg ccgccggtcg tggcggtggt ctgctccatc ggtttcatcg cctcggtgaa
     3661 cccgacaatg gccggcacgc tggtggccgc ttcagcagcg ctcgggatgc tgatcttcta
     3721 cctggccaag cacggcaccc ccctgcaccg ctccttcgcc gccaaggccg ccgcggtcga
     3781 tggcgagctg gtcgatgtcg tcagcaacat gtcagtggtg cgcgcgttcg gcgcgatgtt
     3841 ccgtgagcag cggcgcatcg ccggcacgat cggcgtcgag ctgggcgccc gtcgcagcag
     3901 cctgatctat ctcgagcggc tgcggctgat ccacgcggtc atcaccgcga cgctgatcgc
     3961 cggcatcgtc ggctgggggc tg
//
LOCUS       DATGEM010000518         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_489271, whole genome shotgun sequence.
ACCESSION   DATGEM010000518 DATGEM010000000
VERSION     DATGEM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489271"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /gene="tnpB"
                     /locus_tag="VKQ27_15935"
     CDS             <1..234
                     /gene="tnpB"
                     /locus_tag="VKQ27_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976042.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HLY90473.1"
                     /translation="LGGHLFCFRGRSGNLLKVIWHDGQGACLFTKRLERGRFLWPSPA
                     DGAVTISSAQLGYLLSGIDWRNPQETWRPTSVG"
     gene            complement(252..503)
                     /locus_tag="VKQ27_15940"
     CDS             complement(252..503)
                     /locus_tag="VKQ27_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90474.1"
                     /translation="MREQSCLALRAVAVKLAPQLLDLKLEMSDQRFRIRVHGLRTSRN
                     RLGFQTPSALRQDHRVSGGEIGRKRFSNVFHEKMESHPP"
     gene            393..1925
                     /locus_tag="VKQ27_15945"
     CDS             393..1925
                     /locus_tag="VKQ27_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY90475.1"
                     /translation="MNSNTEALIAHLKLEIEKLRRELYGNSSERKARLLAQMELQLED
                     LEATATEDELAAEQAAVGTRTVRSFQRKRPSRKSFPDHLPRERVVIAAPESCPCCGSG
                     RLSKLGEDITETLEVIPRQWKVIQTVREKFSCRDCEKITQPPAPFHVTPRGFAGPNLL
                     AMILFEKFGQHQPLNRQSERYRREGIGLSVSTLADQVGACTTVLQPLHTLIEAHVLAA
                     ERLHGDDTTVPILAKGKTVTGHIWTYVRDDRPFGSRAPPAAIYYASRDRRHEHPVRHL
                     RAFAGILQADAYGGYNELYEPSRSPGPITSALCWSHARRQFFELADIATNARRGKTAA
                     AISPVALEAVRRIDVLFDIERGFNGESAEERLRVRREKSAPLLTALEAWLRDERARLS
                     RSASVAKPIDYMLKRWDRFTRFIDDGRICLTNNAAERALRGFALGRKSWLFAGSERGA
                     DRAAAMATLIMTAKLNDVDPLAWLADVLSRIADLPRSRLQELLPWNWSAHVRSVAPPQ
                     AA"
     gene            1891..2322
                     /locus_tag="VKQ27_15950"
     CDS             1891..2322
                     /locus_tag="VKQ27_15950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HLY90476.1"
                     /translation="MSGLSRRLKQLDEELLALGEETMLLEELDGFIAGLLVCPDLIKP
                     GEWLPIVWNRDSADQQPAFDNLDHVNRVLGLVMEHYNSVARTLMEHPDRYSPLFSVDT
                     RNGDILWELWIEGFETAVELRPKPGKNSSTLMPIRRPPCAE"
     gene            2322..2573
                     /locus_tag="VKQ27_15955"
     CDS             2322..2573
                     /locus_tag="VKQ27_15955"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HLY90477.1"
                     /translation="MALAEVVCRDQHLTRKELDTLNARAPDNIARWVITLNDWRLANY
                     QPMQGIDPRASSGSTKKVGRNDPCPCGSGKKYKKCCGLN"
     gene            2608..2742
                     /locus_tag="VKQ27_15960"
                     /pseudo
     CDS             2608..2742
                     /locus_tag="VKQ27_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          556 a          884 c          894 g          537 t
ORIGIN      
        1 ctgggcgggc acctgttctg cttccgtggc cgcagcggca atcttctgaa agtgatctgg
       61 catgacggtc agggggcgtg cctgttcacg aagcggctgg agcgagggcg gtttttatgg
      121 ccgagcccgg ccgacggcgc ggttacgatc tcgtcggcgc agctcggtta tctgctgtct
      181 ggaattgact ggcgcaatcc tcaggaaacc tggcgtccga catcggtcgg ctaacagttt
      241 ttgcttgcgg atcatggcgg atgtgattcc atcttctcat gaaagacgtt gctgaatcgc
      301 ttccgtccga tctcgccgcc gctcacgcga tgatcctggc ggagcgcgct gggcgtctgg
      361 aagccgaggc ggttgcggct cgtgcgcagg ccgtgaactc gaatacggaa gcgctgatcg
      421 ctcatctcaa gcttgagatc gagaagctgc ggcgcgagct ttacggcaac agctcggagc
      481 gcaaggcacg actgctcgcg caaatggaat tgcagctgga agacctggag gcgaccgcga
      541 ccgaggatga actggcggcc gaacaggcgg cggtcgggac gcggacggtg cgatcgttcc
      601 agcgcaagcg accctcgcgc aaatccttcc ccgatcatct gccgcgcgag cgtgtggtga
      661 tcgcagcgcc tgagagctgt ccgtgctgcg gatcgggccg gctgtcgaag cttggcgagg
      721 acatcaccga aacgctggag gtgattccgc ggcagtggaa ggtgatccag acggtgcgcg
      781 agaagttctc gtgccgtgat tgtgagaaga tcacccagcc gccggcgccg ttccatgtga
      841 cgccacgcgg cttcgccggg ccgaatttgc tggcaatgat tctgttcgag aagttcggtc
      901 aacaccagcc gctgaaccgg cagagcgagc gataccggcg cgaaggcatc ggtctcagcg
      961 tatcgacgct ggccgaccag gtaggcgcct gtacgacggt gctgcagccg ctccacaccc
     1021 tgattgaggc ccacgttctg gctgcagaac ggttgcacgg cgacgacacg acggtgccga
     1081 tcctggccaa aggcaaaacg gtcaccgggc atatctggac ctatgtccgc gacgatcgtc
     1141 cgtttggcag tcgtgcgccg ccggcggcga tttattacgc ttcgcgggat cggcggcatg
     1201 aacatcccgt ccggcatctt cgagccttcg ccggcattct gcaggccgac gcctatggcg
     1261 ggtacaacga actttacgag ccgtcccgct cgccggggcc gatcacgtcg gcgctgtgct
     1321 ggagccatgc aagacgtcag ttcttcgaac tggccgatat tgctaccaat gcccgacgcg
     1381 gcaagaccgc ggcggcgatc tcgcctgtcg cgctggaggc cgtccggcgc atcgacgtgc
     1441 tgttcgatat cgagcgcggc ttcaatggtg agagcgccga agaacgtctg cgggtgcggc
     1501 gggaaaaaag cgcgcctctt ctgacggcgc tggaagcgtg gctgcgtgac gagcgtgccc
     1561 gcttgtcgcg ctcggcctcc gtcgccaagc cgatcgacta catgctcaag cgctgggacc
     1621 ggttcacgcg attcatcgac gacggcagga tctgcctcac gaacaacgcg gccgagcgcg
     1681 cgcttcgcgg ctttgcactg ggacgcaagt catggctctt tgccggctca gagcgcggtg
     1741 ccgaccgggc cgcggccatg gccacgctga tcatgacggc aaagctcaat gacgtcgatc
     1801 cgctagcgtg gctcgccgac gtgctctccc gcatcgccga cctgccacga agccgcctcc
     1861 aggaactgct gccatggaat tggagcgccc atgtccggtc tgtcgcgccg cctcaagcag
     1921 cttgacgagg aactgctcgc ccttggcgag gaaacgatgc tgctcgaaga gcttgacggc
     1981 ttcattgccg gtttgctggt ctgccccgac ctgatcaagc ctggcgagtg gcttccaatc
     2041 gtctggaacc gggatagcgc agatcagcag ccggccttcg acaaccttga ccatgtcaac
     2101 cgggttctcg gtctcgttat ggagcattac aacagtgtcg cgcgtacgct catggagcac
     2161 cccgaccgct acagtccact gttctccgtc gatacccgta atggcgacat cctctgggag
     2221 ctttggatcg agggcttcga gacggccgtc gagttacgac cgaagcctgg caaaaactcc
     2281 tcaactctga tgccgatacg gcggccgcca tgcgcggaat gatggcgctc gctgaggtcg
     2341 tctgccgcga tcaacacctg acacgaaagg agcttgatac ccttaatgcg agggcccccg
     2401 acaatatcgc gcgctgggtc atcacactca atgactggcg gcttgccaat taccagccaa
     2461 tgcagggtat cgatccgcga gcatcctcag gttcgacgaa aaaggtcggc cgtaacgatc
     2521 cctgtccgtg cggctcagga aagaaataca agaaatgctg cggactcaac taaacgccgc
     2581 cactgcggtg ctcaccggat ggttaccctt caccgctacc tttcagaatt tgacttccgt
     2641 tactcaaacc gcgtcgcgct tggcgtcaac gacggggaac gtgcggacct tgccatcaag
     2701 ggtgcggctg gcaagcgtct cacgtatcgt caacctaact aaccgggact tcaaacgact
     2761 cgccagacgc tttttgcgct ggcgagcaaa gcagccaaaa ccaaggcaga acttcgtcaa
     2821 atactggcgg acgcgctaat ccacaggtgt gcacaaaacg ggaacccaat c
//
LOCUS       DATGEM010000519         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_783332, whole genome shotgun sequence.
ACCESSION   DATGEM010000519 DATGEM010000000
VERSION     DATGEM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783332"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="upp"
                     /locus_tag="VKQ27_15965"
     CDS             <1..615
                     /gene="upp"
                     /locus_tag="VKQ27_15965"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HLY90478.1"
                     /translation="VVDHPLIQHKLTLLRDKRTPTGQFRELAHEISVLMAYEVTRVLP
                     LEPVEIETPLEHMQSARLLGKKLCFVSILRAGNGILEGMLDLVPSARVGHIGLYRDHT
                     TLLPVQYYLKLPDDLPQRLTIVVDPMLATGHSASAAVARVRQAGAQQIRFMCLIAAPE
                     GLRVFHADHPDVPVFTAAIDRCLDENGYIRPGLGDAGDRLYGTK"
     gene            complement(600..1769)
                     /locus_tag="VKQ27_15970"
     CDS             complement(600..1769)
                     /locus_tag="VKQ27_15970"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY90479.1"
                     /translation="MTDAVHAFVPGSRVRIEGASGGPLARLTFAAKDLFDVAGFATGG
                     GNHDWARVHPVPTRHAWAVQRLLDAGATLIGKTITDEVSLGILGENAFDGTPLNTKAP
                     AHVPGGSSSGSAAAVAAGLCDTALGTDTGGSVRVPSSFCGLFGIRPTHGRLDLTGMLP
                     QAPSSDTTGWFARDAATFAKVSSVMLGEAIPDALPSQLIIAVDAFGFADAAVADALRP
                     MVARLGALIGDAREEVMAPPGLSVWSRAQRTLQPVEAWQTFRPWVERDNPRMAFNVAR
                     GLVTGSMISEADRGWAKLMRQEARGRMAYLLPPGTILCLPTTPFPAPKKGLPLPVLGP
                     LRDRISCLCSHGGLTGVPQVSIPGVEVHGLPVGLSIVGGRGTDASLVAVAAHLVP"
     gene            1820..2194
                     /locus_tag="VKQ27_15975"
     CDS             1820..2194
                     /locus_tag="VKQ27_15975"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLY90480.1"
                     /translation="MADNAQMIIELDRKRMTAMAEKDIATLNAVLADDLIYTHSSARL
                     DSKQSLIGNMESGATVYNSVEPSDVKAQDLGSTVVLTGVARIRVTSGGKPNAFGVRFT
                     DVYANKGGKWQMVTWQSTRLPD"
     gene            complement(2232..>2672)
                     /locus_tag="VKQ27_15980"
     CDS             complement(2232..>2672)
                     /locus_tag="VKQ27_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HLY90481.1"
                     /translation="LAGACLTMLTARRAVFFGLDLSGAALQAARCGEADFRDCTFNGA
                     DMRGIVLTGATLNGASMQGVKLRPLIFDGGRRMQSDLSGASLRHVDLRGADLRGVRLS
                     GADMSFADLTGADVTGMDLEGAKLAGCRLSHDLFDSAVLRERTA"
BASE COUNT          537 a          980 c          755 g          400 t
ORIGIN      
        1 gtcgtggatc atccactgat ccagcacaag ctgacgctgc tgcgcgacaa gcgaacgccg
       61 actggccagt tccgcgaact cgcacacgag atcagcgtgc tgatggcata tgaagtgacg
      121 cgcgtgctcc cactcgagcc cgtcgagatc gaaacgccgc tggaacacat gcagtccgcc
      181 cgtctgctcg gcaagaagct ttgcttcgtc tctatcctgc gtgcaggcaa cggcatcctt
      241 gagggcatgc tggacctcgt tccgtccgcg cgtgtcggcc atatcggcct gtatcgcgac
      301 cacaccacgc tgctgcccgt tcagtattac ctgaaactac cggacgacct gccgcagcgt
      361 ctgactatcg tggtggaccc gatgctggcc accggccact cggccagcgc cgccgtcgcc
      421 cgcgtgcgcc aggccggcgc gcagcaaatc cggttcatgt gcctgattgc cgctcccgag
      481 ggcctgcgcg tctttcatgc cgaccatccc gatgtaccgg tcttcaccgc cgccatcgat
      541 cgctgcctgg atgagaacgg ctatatccgt cccggcctcg gcgacgccgg cgatcgtctc
      601 tacggcacca agtgagccgc cacagcgacg agcgaggcgt cggtgccacg cccgcccacg
      661 atcgataatc caaccggcaa cccatggacc tcgacgccag gaatgctgac ctgcggcaca
      721 ccggtcagtc cgccgtgcga acagaggcac gagatgcgat cgcgcaacgg tcccagcacc
      781 ggcagcggca accctttctt cggtgccgga aatggcgttg tcggcaagca gaggatggtg
      841 cccggcggca gtagatacgc catgcgtccg cgcgcctcct gccgcatcag cttggcccag
      901 ccacgatccg cctcgctgat catcgagccc gtcaccaacc cgcgcgccac gttgaacgcc
      961 atacgcggat tatcgcgttc cacccagggg cgaaatgtct gccacgcctc caccggctgc
     1021 agcgtgcgct gcgcacgcga ccagacggaa agccctggcg gcgccatcac ctcttcccgc
     1081 gcatcgccaa tcagcgcgcc cagccgcgcc accatcggcc gcagcgcgtc ggccaccgca
     1141 gcatccgcaa agccgaacgc atcgacagcg ataatcagct gcgatggcag ggcatcgggg
     1201 atcgcctccc ccagcatcac gctcgacacc ttggcgaacg tcgccgcatc gcgcgcgaac
     1261 cagccggtgg tgtcagaact cggcgcctga ggcagcatac ccgtcaggtc cagccgccca
     1321 tgtgtcggcc ggataccgaa cagcccgcag aaactcgacg gcacccgcac cgatccaccg
     1381 gtgtccgtgc ccaacgcggt atcacacagc cccgcggcca ccgcggccgc cgacccagac
     1441 gacgaaccac ccggcacatg agccggcgcc ttggtgttca gcggcgtccc atcgaatgca
     1501 ttctcaccaa ggatccctaa cgacacctca tcggtgatcg tcttgccgat cagcgttgcg
     1561 ccggcatcca gcaacctctg caccgcccag gcatgccgcg tcggcacagg atggacgcgc
     1621 gcccaatcgt gattgccgcc gcccgtcgca aatcccgcaa catcgaacag atctttcgcc
     1681 gcgaatgtca gccgggcgag cggcccaccg gaagcgccct cgatgcgcac acgggaccca
     1741 ggaacgaagg catgcacggc gtctgtcatc tgatcccctg tcacgctaca cttgctgcgc
     1801 agcataggga gtgaaacaca tggccgacaa cgcacagatg atcattgagc tcgatcgcaa
     1861 gcgcatgacc gcaatggccg agaaggacat cgccacgctg aacgcggtgc tcgcggacga
     1921 cctgatttac acgcactctt cggcgcgcct cgactccaag cagagcctta tcggcaacat
     1981 ggaatcaggg gcgacggtct acaattcggt cgaaccgtcc gatgtgaagg cgcaggacct
     2041 tggcagcacg gtcgtgctga ccggcgtggc ccgcattcgc gtcacctctg gcggcaaacc
     2101 aaacgccttt ggtgtccgat tcacagacgt ctacgccaac aagggcggca aatggcagat
     2161 ggtcacctgg caatccaccc gcctgccgga ctgacgccac caaagccagc acaacgcaag
     2221 gacggcgtcc gttacgccgt cctctcgcgc aacacggcac tgtcgaacag atcgtgcgaa
     2281 agccgacaac cagccagctt cgcgccctcg agatccatgc ctgtcacgtc ggcgccagtc
     2341 agatcggcaa aagacatatc ggcgccactc agccgcacgc cgcgcaaatc ggcaccgcgc
     2401 agatccacat gtcgcaaact tgcacccgac agatcggact gcatccgccg cccgccatcg
     2461 aaaatcagtg gtctcagctt cacgccctgc atcgaggcgc cgttgagcgt cgcgcccgtc
     2521 agtacgatgc cgcgcatgtc ggcgccattg aacgtgcagt cgcggaaatc cgcctcaccg
     2581 caacgcgccg cctgcaatgc cgcaccagac agatcgagcc caaagaacac cgcgcgccgc
     2641 gctgtcagca tggtcaggca cgcgcccgca ag
//
LOCUS       DATGEM010000520         6550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_201365, whole genome shotgun sequence.
ACCESSION   DATGEM010000520 DATGEM010000000
VERSION     DATGEM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201365"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2321
                     /gene="clpB"
                     /locus_tag="VKQ27_15985"
     CDS             <1..2321
                     /gene="clpB"
                     /locus_tag="VKQ27_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HLY90482.1"
                     /translation="LVRLLDAAQQAATKAGDEYVAQDRLLVAIAASDTSAARALRQAG
                     ATPQALEKAVNDIRKGRKVTSANAESTFDALKKYARDMTALARDGKLDPVIGRDEEIR
                     RTIQVLARRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPEALKGKQLLSLDLGAMV
                     AGSKYRGEFEERLKAVLKEIESAEGQVVLFIDELHNLMGAGRAEGSMDAANLIKPELA
                     RGTLHCIGATTLDEYRKHIEKDAALARRFQPVFVGEPSVEDTISILRGIKEKYELHHG
                     VRITDGALVASATLSNRYITDRFLPDKAIDLVDEAASRLRMQVDSKPEELDELDRRVI
                     QLKIEREALKKETDSASRDRLGKLEKELAELEDESNALTAAWHAEKGKVQETQKLKEQ
                     LDAAKSEVEIAQRRGDLARASELMYGVIPGIEKQLASSADGKLVNEAVTEEQIAQVVS
                     RWTGIPVDKMMEGERDKLLRMEEMLRQRVVGQDEALKAVANAVRRARAGLQDPNRPIG
                     SFLFLGPTGVGKTETCKALAEFLFDDERAMVRIDMSEFMERHAVARLIGAPPGYVGYD
                     EGGVLTEAVRRRPYQVILFDEVEKAHEDVFNVLLQVLDDGRLTDGQGRTVDFKNTIIV
                     LTSNLGSEVLAAQPEGEPTAMVQGQVMRIVRQHFRPEFLNRLDEIILFRRLQRVDMSS
                     IVNIQLAHLRKLLAERKIGIELDRTALEWLGSEGYDSTYGARPLKRVIQRGLQNPLAS
                     LILEGSVKEGDTVHVSAGPNGLTINGQLAEAA"
     gene            complement(2464..3408)
                     /locus_tag="VKQ27_15990"
     CDS             complement(2464..3408)
                     /locus_tag="VKQ27_15990"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HLY90483.1"
                     /translation="MGLFTAIRLDAYPSQPFAALPTDTAFSLRVAGALAWAAQLAYEV
                     LTPDKFSQVLRQWQWTNPQTIQAADSPVVGSSRTKGFAATWSDCTIIAFAGTEPNQFS
                     DWKEDFTILRDGRGAHEGFENGLDAVWTTVERIARESAPGGLYLTGHSLGGALAGVAA
                     RRLIDQRIIDADRIKGVYTIGMPRIGDADYAQAYDAAGGQRLGQRTFRLVHGQDIVTK
                     VPPTTLDFAHVGCMLACPRGGRFPAATPTPTIERPGTDELFDAAWSLLDLPIFSTDDG
                     LPAYPSPEHAIAMAVATLPKPIRDHLPDRYLAALPALN"
     gene            3555..4295
                     /locus_tag="VKQ27_15995"
     CDS             3555..4295
                     /locus_tag="VKQ27_15995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90484.1"
                     /translation="MTQNIYDNPEFFAGYSQLGRSVEGLDGAAEWPALRALLPDLNGR
                     SVVDLGCGFGWFCRWAREQGAARVLGLDVSDKMLERARADTADAAISYQRADLEQLEL
                     PEAAFDLAYSSLALHYVEQLDKLLATVHRALKPGGTLVFSAEHPIYTAPSRPGWMADA
                     SGRRTWPVDGYLVEGPRTTDWLAKGVIKQHRTIGTYLNLLLRLGFRLMHVEEWGPTDA
                     QIASRPALADERQRPPFLLVAARKAGAG"
     gene            complement(4230..5591)
                     /gene="cysG"
                     /locus_tag="VKQ27_16000"
     CDS             complement(4230..5591)
                     /gene="cysG"
                     /locus_tag="VKQ27_16000"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HLY90485.1"
                     /translation="MRHFPAFLNLAGRQALVIGSGEIAQRKAAVLASAGALVRTAHSF
                     SDELLQDCAIAIGADAPDAELIALSLAARRRGIPVNVVDRPELCSFIMPALVDRDPVM
                     VAISTGGTSPVLARLLRQQIERLIPPAIGRLAMLIGEVTAEIRRRWPDFSRRRRVLER
                     LLTGRVAELVFAGEDAAAREAVRCEMGSHEANPDCIGTVFLVGAGPGAADLLTLRAHR
                     LLGEADVVVHDRLVGTEVLALARRDAELIDVGKTPGQPCMPQDAISELLVRLAYDGRK
                     VVRLKGGDPFIFGRGAEEIDALTRAGVPFDVVPGVTAALACAAQARIPLTRRGTARSV
                     TFATGHTKDGVPDIDFASATKHGGTLAIYMGIRTLPQLRDGLLRHGMAADMPAVVIEN
                     GGTPKQRELHGTLASIAADAADWSRGGPAMLLLGEAVGRGLAGASLARSHQQEGRALA
                     FVR"
     gene            complement(5591..>6550)
                     /locus_tag="VKQ27_16005"
     CDS             complement(5591..>6550)
                     /locus_tag="VKQ27_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90486.1"
                     /translation="GDSLGLAVPNDPALVEAILAALGTSDDDVRAALTTELDIARPLD
                     RTLDLLAGAAVHPRQAAALRALSAGETGAEPTDADLLDLLEAFPSARPPLADLLKSLP
                     PLRPRIYSIASSQRVTPTEVHLCVAVVRDMRRGRLRHGVASAFLADRAIQHGPVRGFI
                     QTSHFRLPADPRTHIIMCGPGTGVAPFRAFLQERAALGIKGRAWLLFGERQRAHDFLY
                     EAELKTWLADGTLSRLDVAFSRDQSAKDYVQHRMAENATDLWRWLQEGACFYVCGDAT
                     RMARDVDAALRRVAMTEGKLDESQARDWIIALARQGRYQRDIY"
BASE COUNT         1195 a         2073 c         2216 g         1066 t
ORIGIN      
        1 acctggtgcg gttgctggat gccgcgcagc aggccgcgac caaagccggc gacgaatatg
       61 tggcgcagga ccggctgctg gtggcgatcg ctgcctccga cacttcggcc gcccgcgcgc
      121 tgcgccaggc tggtgccacg cctcaggcgc tggagaaggc ggtcaacgac atccgcaaag
      181 gccgcaaggt caccagcgcc aatgccgaat ccacgttcga cgcgctgaag aaatacgccc
      241 gcgacatgac ggcgctggcg cgcgacggca agctggaccc ggtgatcggc cgcgacgagg
      301 aaatccgccg caccatccag gtgctggcac gccgcaccaa gaacaacccg gtgctgatcg
      361 gcgagcctgg cgtgggcaag accgccatcg tcgaagggct cgcgctgcgt atcgtcaacg
      421 gcgacgtgcc cgaggcactg aagggcaagc agcttctgtc gctggacctc ggcgcgatgg
      481 tcgcaggatc gaagtaccgc ggcgagttcg aggaacgcct gaaggccgtg ctgaaggaga
      541 tcgagtcggc cgagggccag gtcgtgctgt tcatcgacga actgcacaac ctgatgggcg
      601 ccggtcgcgc cgaaggctcg atggacgcgg cgaacctgat caagccggag ctcgcgcgcg
      661 gcactttgca ttgcatcggc gctaccacgc tggacgagta ccgcaagcac atcgagaagg
      721 acgcggcgct ggcgcggcgc ttccagccgg tgttcgtggg cgaaccatcc gttgaggaca
      781 cgatttcgat cctgcgcggc atcaaggaaa agtacgagct gcatcacggc gtccggatca
      841 ccgatggtgc actggtggcc tcggcgacct tgtccaaccg ctacatcacc gaccggttcc
      901 tgccggacaa ggcgatcgac ctggtggatg aggcggcgag ccggctgcgc atgcaggtgg
      961 attccaagcc tgaggaactg gacgaactcg accgtcgggt catccagctc aagatcgaac
     1021 gcgaggcgct aaagaaggag acagactcgg catcgcgcga ccggctgggc aagctggaaa
     1081 aggagctggc cgaactcgag gatgagtcga atgcactgac cgccgcgtgg catgcggaaa
     1141 agggcaaggt gcaggagacc cagaagctga aggagcagtt ggacgcggcg aagagcgaag
     1201 tggagatcgc acagcgccgc ggcgatctgg ctcgtgcatc ggagctgatg tacggcgtga
     1261 tccccggcat tgaaaagcag ctcgcatcgt ccgccgacgg caagctggtg aacgaggcgg
     1321 tcaccgagga gcagatcgct caggtggtgt cgcgctggac cggcattccc gtcgacaaga
     1381 tgatggaagg cgagcgcgac aagctgctgc gcatggaaga gatgctgcgt cagcgtgtcg
     1441 tcggacagga cgaggcgctg aaggcggttg ccaacgcagt gcgccgcgca cgcgccggct
     1501 tgcaggatcc gaaccggcca atcggctcgt tcctgttcct gggcccgacg ggcgtcggca
     1561 agaccgagac ctgcaaggcg ctggccgagt tcctgttcga tgacgagcgc gccatggtgc
     1621 gcatcgacat gagcgagttc atggagcgcc atgcagtggc ccggctgatc ggtgcgcctc
     1681 caggctatgt cggctacgac gaaggcggtg tgctgaccga ggcggtgcgt cgccggccat
     1741 accaggtgat cctgttcgac gaggtcgaga aagcgcatga ggacgtgttc aacgtcctgc
     1801 tgcaggtgtt ggacgatggc cgtctgaccg atggccaggg ccgcacggtg gacttcaaga
     1861 acaccatcat cgtgctgacc tccaaccttg gctccgaggt cctggccgcg cagcccgagg
     1921 gtgagccaac ggccatggtg cagggccagg tgatgcggat cgtgcggcag catttccggc
     1981 ctgagttcct gaaccggctg gacgagatca tcctgttccg ccgtctgcag cgcgtcgaca
     2041 tgtcgagcat cgtcaacatc cagctggcgc atctgcgcaa gcttctggcc gaacggaaga
     2101 tcggcatcga gctcgatcgg accgcgttgg agtggctcgg gtccgagggt tacgacagca
     2161 cgtacggcgc gcgtccgcta aagcgcgtga tccagcgtgg gctgcagaat ccattggcgt
     2221 cgctgatcct ggagggctcg gtgaaggagg gcgacacagt gcacgtctcg gccgggccga
     2281 acggcctgac gatcaacggg cagctggccg aagcggctta aggcgtgggt ttggggtcgg
     2341 tgaggtgctg gttctctggt gcctcacccg ctcccttcgc cggaattgtc gtagggcact
     2401 ggtgatcggc ttgaccacgc caaatgcgcg ccgcacgggc cggcagcagt atctgtcgtc
     2461 caatcagttg agggcaggca gggcggcgag gtatcgatcc ggcaggtgat cgcggatcgg
     2521 cttcggcaat gttgcgacgg ccatcgcgat cgcatgttcg ggcgagggat aggctggcag
     2581 tccatcgtcc gtggagaaga tcggcagatc aagcagcgac caggccgcgt cgaacagttc
     2641 atcagttccc ggccgctcga tggtgggcgt cggcgtggca gccgggaatc tgccaccgcg
     2701 cggacaggcg agcatgcatc cgacatgcgc gaagtcgagt gtcgtgggag gcaccttggt
     2761 gacgatgtcc tgcccgtgga caagccggaa cgtacgctgc cccaatcgtt ggccaccggc
     2821 tgcatcgtaa gcctgggcat agtcggcgtc gccgatgcgt ggcatgccga tcgtatatac
     2881 ccccttgatc cggtcggcgt cgatgatgcg ctggtcgatc aaccggcgcg cggcgacgcc
     2941 tgccaacgcg ccacccaaac tgtgacccgt caggtaaagt cctccaggcg cggactctct
     3001 cgcgatcctt tccacggtgg tccaaacggc atcgaggccg ttctcgaatc cttcgtgcgc
     3061 gcccctgcca tcgcgcaaga tggtgaaatc ctctttccag tcgctgaatt ggtttggttc
     3121 ggtgccggcg aatgcgatga tcgtgcagtc gctccacgtc gcggcaaagc ctttcgtccg
     3181 gctggaaccc acaacaggac tatcggccgc ctggatggtc tgcgggttgg tccactgcca
     3241 ttgacggaga acctggctga atttgtccgg cgtcagcact tcgtatgcaa gctgtgcggc
     3301 ccacgccaag gcgccggcaa ctcgcagcga gaacgcagtg tccgtcggca aggcagcaaa
     3361 gggctgcgag ggatacgcgt ccagccggat tgcggtgaac aggcccatgg tcaagctccg
     3421 ttctgcggac ctcacgctat cgcaatggat gcgcattgca cacaaatttg ttcgcggcgg
     3481 tctccggcgc ttgccttcgt gacccgcgcg ggcatgcctt gacggcgagg gcgcatccgg
     3541 tcgatggggc ggcgatgacc cagaacattt acgacaaccc cgagttcttc gctggttaca
     3601 gccagttggg ccgttcggtc gaaggattgg atggcgcggc cgaatggccg gcgctgcgcg
     3661 cactcttgcc tgatctaaac ggacgcagcg tggtggatct tggctgcggc ttcggctggt
     3721 tctgccgctg ggcacgcgag cagggtgcgg cacgggtgct ggggctggat gtgtcggaca
     3781 agatgctgga gcgagcacga gcagataccg cggatgcagc gatcagctat cagcgggctg
     3841 acctcgagca gctggaattg ccagaggcag cgttcgacct cgcttacagt tccctggcac
     3901 tgcattacgt ggaacagctc gacaaactgc tcgcgaccgt ccaccgcgcg ctgaagccgg
     3961 gcggcacgct ggtgttctcg gcggaacatc cgatctacac ggcgccctcg cggcctggtt
     4021 ggatggccga cgcgtcaggg cgacgcacct ggccggtgga tggctatctt gtggagggtc
     4081 cacgcaccac ggactggctg gccaagggcg tgatcaaaca gcaccgcact atcggcacct
     4141 atctgaacct gttgctgcgg ctggggttcc gcctgatgca cgtagaggaa tggggaccga
     4201 cggatgcgca gatcgcatcc cgcccggcgc tagcggacga acgccaacgc ccgcccttcc
     4261 tgctggtggc tgcgcgcaag gctggcgccg gctaggccac gcccgactgc ctcgccgagc
     4321 aacagcatgg cagggccgcc gcgcgaccag tccgctgcat cggcagcaat gcttgccagc
     4381 gtgccgtgca gttcgcgctg cttgggcgtg ccgccgttct cgatcacaac ggctggcatg
     4441 tctgcggcca tgccatggcg cagcaatcca tcccgcagtt gcggcaacgt gcggataccc
     4501 atgtagatcg ccagtgtgcc gccgtgcttc gtcgcactgg cgaagtcgat gtcgggcacg
     4561 ccgtccttgg tatgacccgt ggcgaatgta accgagcgcg ccgtaccgcg tctggtcagc
     4621 gggatacgcg cctgtgccgc gcaggcaagc gccgcggtga cgcctggcac cacgtcgaac
     4681 ggcacgccgg cgcgcgtcag cgcatcgatc tcctcggcgc cacgcccgaa gatgaacgga
     4741 tcgccgccct tcagtcgcac caccttgcgc ccgtcatacg caagccgcac cagcaattcg
     4801 ctgatcgcgt cctgcggcat gcatggttgg ccaggtgtct tgccaacgtc gatcagctcg
     4861 gcgtcgcgcc gggcaagtgc aagcacctcg gtgccgacaa gccggtcgtg caccacgacg
     4921 tccgcctcgc cgagcagacg atgcgcgcgc aatgtcagca gatcggctgc tcccggaccc
     4981 gcgccgacaa ggaacaccgt gccgatgcag tcggggttcg cttcatgcga gcccatctcg
     5041 catcgcaccg cctcacgtgc ggccgcgtcc tcgccggcga acaccagttc ggcaacgcgc
     5101 ccggtcagca gtcgctcgag cacgcgccgg cggcgcgaga aatcgggcca gcgtcggcgg
     5161 atctctgccg tgacctcgcc aatcagcatc gccagccgcc cgatcgccgg cgggatcagg
     5221 cgttcgatct gctggcgaag cagccgtgcc agcacgggag acgtaccgcc ggtcgagatc
     5281 gcgaccatga ccggatcccg atcgaccaag gccggcatga tgaagctgca cagctcggga
     5341 cgatcgacaa cgttcaccgg aatgcctcgc ctgcgtgccg caagcgacaa tgcgatcagt
     5401 tcggcgtctg gcgcatcggc gccgatggcg atcgcgcagt cctgcaacag ctcatcgctg
     5461 aagctgtgtg cagtgcggac aagggctcct gccgatgcca gcaccgccgc cttgcgttgc
     5521 gcgatctcgc ccgaaccgat gaccagcgcc tgtcggccgg cgaggttcag gaaggcagga
     5581 aaatgccgca tcagtagata tcgcgctggt aacggccctg ccgtgccagc gcgatgatcc
     5641 agtcgcgtgc ctgcgattca tcgagcttgc cttcggtcat cgcgacgcgt cgcaacgcgg
     5701 catcgacgtc gcgcgccatg cgcgtcgcgt caccgcagac atagaagcac gcaccttcct
     5761 gcagccagcg ccacaggtcc gtggcgttct cggccatgcg gtgctgcacg tagtccttcg
     5821 ccgactgatc gcgcgagaac gcgacatcga ggcgcgacag tgtgccgtcg gcgagccatg
     5881 tcttcagttc tgcctcgtaa aggaaatcgt gggctcgctg gcgttcaccg aacagcagcc
     5941 aggcgcgacc cttgatgccg agtgccgcgc gttcttgcag gaaggcacgg aacggcgcga
     6001 cgcctgtgcc aggaccgcac atgatgatgt gggtgcgcgg atccgccggt agacggaaat
     6061 gactggtctg gatgaagcca cgcaccggcc cgtgttgtat cgcccgatca gcaaggaacg
     6121 cgctggccac gccatgacga agcctgccac ggcgcatgtc acgcacaacc gcgacgcaga
     6181 gatgcacctc ggtcggtgtc acgcgctgcg atgaggcgat ggaataaatc cgcggacgca
     6241 gcggcggcaa tgacttcagc aggtcagcca gcggcggacg ggcggagggg aacgcctcga
     6301 gcaggtcgag cagatcagcg tccgtcggtt cggcgccggt ctcgcccgcc gacagtgcac
     6361 gcagcgccgc ggcctgcctg ggatgcacgg ctgcaccggc gagcagatcg agcgtccgat
     6421 cgagcggccg agcgatatcg agttctgttg tcagcgcagc gcgcacatcg tcgtccgatg
     6481 tgccaagcgc ggcgagtatt gcctccacca gagccggatc attcggcacg gcaagaccca
     6541 gcgagtcgcc
//
LOCUS       DATGEM010000521         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1285620, whole genome shotgun sequence.
ACCESSION   DATGEM010000521 DATGEM010000000
VERSION     DATGEM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285620"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VKQ27_16010"
     CDS             complement(<1..1085)
                     /locus_tag="VKQ27_16010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLY90487.1"
                     /translation="MVGTAIAMASTAYPLWAQAQDATDQSSNSEVKGLQEVVVTAEKQ
                     ATGRSVQKVPIAITALDAETMAEQHIESLSDVGHLAPGVTLDQMGTLPGSAGFEIRGI
                     SLRSSSSSIDLPNAVMQDGMPLTLQNGVTYLGTFDTQSVEVLRGPQGVLQGVNAAGGA
                     VTFTTPLPSSTFHASGSFTFGNFDEVKASGVVEGPLDDIISAKIAVAETHIDGYYNNT
                     TGQGTYKVAPGNPSGVAPQYPTGLIGGTQSVVVKPTFLIEPNEDMKIKIFAQFEDDIM
                     GGSSPIAINPNPAPGPASKFLTVFGYTPSVQPYQIAIATPGFSHNSEEHVIGEFDWNV
                     AGGSWTTIASFRNVNYNALYNAAGSPF"
     gene            1410..2642
                     /locus_tag="VKQ27_16015"
     CDS             1410..2642
                     /locus_tag="VKQ27_16015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HLY90488.1"
                     /translation="MTIVSPDLPIEAGQPSQFVRGPGEAMARGMQRYLSLDDFEAAAR
                     RHLPKLLYEFIRGGAETEATLRGNRQAFHDYGLVPRVLRDVSQRDHSVQLLGKTYAAP
                     FGIAPMGSASLYAYRGDIALGRAAGAMNVPMVLSAASLITLEDVKREAPTAWFQAYLA
                     GESSHIEPLVDRVAAAGYDTFVVTADVPVPANRENNLRLGYKMPLAVTPRVAWDAAIH
                     PGWLFGTWMRTLIKHGMPHFENFDATRGAPLLSKDLVRRIGKRDQLTWKHVESIRRRW
                     QGKLVVKGLIAPQDARIARDCGADGVIVSNHGGRQLDGVVSPLCTLPEIAAEANGMAV
                     MLDGGVRRGTDVLKALALGANFVFVGRPMLFAAAAGGEQGVRHALALLKAEIDRNMAL
                     LGTRSLAEITPDHVIRRG"
BASE COUNT          496 a          794 c          866 g          561 t
ORIGIN      
        1 ttgaagggcg acccggcggc attgtagagg gcgttgtaat tgacgttgcg gaacgaggcg
       61 atggtggtcc agctgccgcc cgccacgttc cagtcgaact cgccgatcac atgctcttcg
      121 ctgttatgcg agaagcccgg ggtggcaatg gcgatttggt aaggctgtac cgagggcgta
      181 tagccgaaga ccgtgaggaa cttgctggcg ggtcccggag cgggattggg attgatggcg
      241 atgggcgagg agccgcccat gatgtcatcc tcgaactggg cgaagatctt gatcttcata
      301 tcctcgttcg gttcgatcag gaaggtgggc ttgaccacca cggactgagt gccgccgatg
      361 aggccggtcg gatattgcgg cgccactccg gacggatttc ccggcgcaac tttataggtc
      421 ccctggccgg ttgtgttgtt gtaatagccg tcgatatggg tttcggccac ggcgatcttg
      481 gccgaaatga tgtcatcgag ggggccctcg acgacgcccg aggctttgac ctcgtcgaaa
      541 ttgccgaacg tgaacgagcc gctcgcatgg aaagtcgagc tcggaagggg ggtggtgaag
      601 gtcacggcgc cccccgcggc gttgacccct tggaggacgc cctgcggtcc gcgcaggact
      661 tcgaccgact gggtgtcgaa ggtgcccaga taggtcaccc cgttttgaag ggtcagcggc
      721 atgccgtcct gcatgacggc attcggcaaa tcgatcgaac tggatgacga gcgcaacgag
      781 atgccgcgaa tctcgaatcc ggcggatccg ggcagggtcc ccatctggtc cagggtcacg
      841 cccggcgcga gatgtccgac atcggagagg ctttcgatat gttgctccgc catggtctcg
      901 gcatcgaggg cggtaatcgc gatcggcact ttctgaacag agcgccccgt cgcctgcttt
      961 tccgccgtta ccacgacctc ttgcaagccc ttcacttcgg aattgctcga ttgatcggtc
     1021 gcatcctgcg cctgcgccca tagtggatag gcggtgcttg ccatggcaat ggcggtgccg
     1081 accaacaagg aaactctacg tcctggcatg tgaatcctcc gcatttttat ttatgcgccc
     1141 ccagcattca cttgtggcgg gggcctcccg tggcatcgga actgagttcc agtttttgga
     1201 aaacaccatg caagcccggc gctggcccgt caacttaatt atgcataaaa actataaata
     1261 tgcatttaaa ggtgagcggc tcgtccgccc acgctggtta ttgcatgata taatatttta
     1321 tgcataaaat tgccaagatt caccgatcgg cgggctcggt gtgatcgggc ggttgcccgc
     1381 ccgctgtccc ggccgcatga aaaggcatca tgacgatagt ttcccccgat ctacccatcg
     1441 aagccgggca accctcccag ttcgtgcgcg gaccggggga ggccatggcg cgcggcatgc
     1501 agcgctatct gtcgctggat gatttcgagg cggcggctcg acggcatctt cccaaattgc
     1561 tgtatgaatt catccgtggc ggcgcggaaa ccgaagcgac tttgcgcggc aaccgtcaag
     1621 cctttcacga ctatgggttg gttccacgtg tgctgcgcga cgtctcgcag cgtgaccaca
     1681 gcgtccaact gctcggcaag acatacgcgg caccgttcgg gatcgcaccg atgggatcgg
     1741 cctcacttta cgcctatcga ggagacatcg ctctcggccg cgccgccggc gccatgaatg
     1801 ttccgatggt gctcagcgcc gcgtccctca tcacgctcga agacgtcaag cgcgaagcgc
     1861 caacggcctg gttccaagcc tatcttgccg gggagtcgtc gcacatcgag ccgctcgtcg
     1921 atcgggtggc cgcggcgggc tacgacacct ttgtggtcac tgcggacgtg ccagtcccag
     1981 ccaatcgcga gaacaatctg cgccttggat acaagatgcc tctcgccgtc acgccacgcg
     2041 tcgcgtggga cgcagccatt catccgggtt ggctgttcgg cacttggatg cgcacgctga
     2101 tcaagcatgg catgccacac ttcgagaatt tcgatgcgac gcgtggcgcg ccgttattgt
     2161 ccaaggatct tgtgcgcagg atcggaaagc gggatcagct cacctggaag catgtcgagt
     2221 cgatccgccg gcgctggcag ggcaagcttg tcgtcaaggg cctgattgcg ccacaggacg
     2281 cccgcatcgc acgtgactgt ggcgctgacg gcgtgatcgt ctcgaaccat ggcggccggc
     2341 agctcgacgg cgtggtctcg ccattgtgca ctctgccgga gatcgcggcc gaggcgaatg
     2401 ggatggcggt catgctcgat ggcggcgtgc ggcgtggcac cgatgtgctg aaggcgctcg
     2461 cgctcggcgc aaatttcgtc ttcgtcgggc gccccatgct gttcgcggcg gcggctggag
     2521 gcgagcaggg tgtgcggcac gctctggcgt tgttgaaggc agaaatcgat cgcaacatgg
     2581 ccttgctggg gacaaggtcg ctcgccgaaa tcacgccgga tcatgtcatc cggcgaggct
     2641 aattccgatc ggtggctggc cgtctcattg tttggcgctc agcagttcga gaacatatcg
     2701 ctcggtaagc tcgatat
//
LOCUS       DATGEM010000522         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1350029, whole genome shotgun sequence.
ACCESSION   DATGEM010000522 DATGEM010000000
VERSION     DATGEM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350029"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VKQ27_16020"
     CDS             complement(<1..857)
                     /locus_tag="VKQ27_16020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY90489.1"
                     /translation="MKQSLALSVIVVGYNMARELPRTIRSLSPALQRDIDEADYEILV
                     VDNGSATPPTPDEVRSWSPNARLCAIENPTVSPVPAINAALDEARGALVGVFIDGARM
                     ASPRLLAGALEASHVHPRAVVGTLAFHLGPDVQLRSVHAGYDQAMEDRLLDEARWQED
                     PYRLFDISAFAGSSAAGWFVTPAETNALFLSAAHWRELGGYDPRFVTPGGGLANLDLW
                     ERLCADPSARVILLLGEATFHQVHGGIATNSRGSPWELFHQEYISIRGRNFEAPKIQP
                     LLIGEIHSK"
     gene            complement(854..2167)
                     /locus_tag="VKQ27_16025"
     CDS             complement(854..2167)
                     /locus_tag="VKQ27_16025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90490.1"
                     /translation="MQKCYSRFVAGGPLGTQEEVRILDVGGADVNGSYRQIFDHPRFA
                     YTAADISDGPGIHVVLDDPYRLPFDDRSMDIVLSGQMLEHCEFFWLSFAEMVRVLRPS
                     GYLFLIAPSAGPEHRYPVDCYRFYPDAYRALARHANCALVDVWQDERGPWKDLVGVFQ
                     RHGPPSSAAALPQKTAVAPEAIIPGTEQEERVQGQVDYLQVLGDLHGSLSPRCYLEIG
                     VRHGRSLALARGPAIGVDPQPALQVELPATTRVFAQASDDFFASTAGDSLPAPVDLAF
                     IDGLHLFEAALRDFMHIERIAAPGAVIAVDDILPNHPAQAERDRRTRVWTGDVWKLAL
                     ILRRYRPDLTMVLLDTAPSGCLLVGGLDPANRGLWDAYNPIVREAQGWTEIPAELLGR
                     AGAISPLSPAYTGFIAALAEGADTPHSRIEAMRALGAADGFGVRS"
     gene            complement(2248..>2726)
                     /locus_tag="VKQ27_16030"
     CDS             complement(2248..>2726)
                     /locus_tag="VKQ27_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLY90491.1"
                     /translation="STGKTTFLNALLREIPARERLVVIEDTPELKVVHENAVGLLCAR
                     SALSEAAVSAEDLLIASLRMRPDRIILGEIRGREAETFLRAVNTGHPGSLSTIHADSP
                     SRAIDQLALLVLQTGIRMSYEDVVTYVHRSIDVVVQLERTPAGRHLSDILYNDVFG"
BASE COUNT          534 a          912 c          862 g          418 t
ORIGIN      
        1 cacttcgaat ggatctcccc gatcaggaga ggctgaatct tcggtgcttc gaagtttctc
       61 ccgcggatcg aaatatattc ttgatggaat aattcccatg gcgatccacg gctgttggtc
      121 gcgatgccgc catgcacctg atgaaaagtg gcctccccca gcaggagaat gacacgggcc
      181 gaaggatcgg cgcacagccg ctcccagaga tccagattgg ccagcccgcc gcccggcgtc
      241 acgaagcggg gatcatagcc cccgagctcg cgccaatgcg ccgccgacag gaagagcgca
      301 ttggtctccg ccggcgtcac gaaccagccc gccgccgagg atcccgcgaa ggccgagatg
      361 tcgaacaggc gatacgggtc ttcctgccag cgcgcctcat ccagcaggcg atcttccatc
      421 gcctgatcat agccggcatg cacgctgcgc aattgcacgt ccggcccgag atggaaggcc
      481 agcgtgccga caaccgcgcg cgggtgcaca tgcgacgctt cgagcgcgcc ggcgagcagc
      541 cgcggcgagg ccatgcgggc cccgtcgatg aaaacaccca ccaatgcgcc ccgcgcttcg
      601 tccagcgccg cattgatcgc cggaacgggc gaaaccgtgg ggttctcgat ggcgcagagg
      661 cgggcattgg gcgaccatga ccggacctcg tccggggtgg gcggcgtcgc cgagccgtta
      721 tccacgacaa ggatctcata atccgcctcg tcgatgtcgc gctgcagggc gggcgacagg
      781 ctgcggatcg tgcgcggcag ttcgcgcgcc atgttgtatc cgacgacaat gaccgaaagc
      841 gccagggact gcttcacgaa cgcactccaa aaccatctgc tgcgcccagg gcacgcatcg
      901 cctcaatccg ggaatgcggc gtatcggccc cttcggcgag cgccgcgata aagcctgtgt
      961 acgccggaga caaaggcgaa atggcgccgg cccgtcccag cagctctgcc ggtatttcgg
     1021 tccagccctg tgcttcgcgg acgatgggat tataggcatc ccacagcccg cgattggccg
     1081 ggtcgaggcc gccgaccagc aggcagcccg acggcgcggt gtcgagcagc accatggtca
     1141 gatcgggccg ataccgacgc aggatcaggg ccagcttcca gacatcgccc gtccagaccc
     1201 gggtgcggcg atcgcgctcc gcctgcgccg gatgattggg caggatatcg tcgacggcga
     1261 tcaccgcccc tggcgccgcg atccgctcga tatgcatgaa gtcgcgcaag gcggcttcga
     1321 acagatgcag gccatcgatg aaggcgagat cgaccggagc cggcagcgaa tctcctgcgg
     1381 tcgaggcgaa gaaatcatcg ctggcctgcg cgaaaacccg cgtcgtcgca ggcagttcga
     1441 cctgcaacgc gggctgcgga tccacgccga tcgccggacc tcgcgccagc gcaaggctgc
     1501 ggccatggcg cacaccgatc tccaggtagc agcgcggcga gagcgatccg tgaagatcgc
     1561 ccaggacctg caaatagtcc acctgcccct ggacgcgctc ctcctgctcc gtgcccggta
     1621 tgatcgcctc gggcgccacg gccgtctttt gcggaagcgc cgcggccgaa gacggcggcc
     1681 catggcgctg gaagaccccg acaagatcct tccacggccc gcgttcatcc tgccacacat
     1741 cgaccagcgc gcaatttgca tggcgggcca gcgcccggta ggcgtcggga tagaagcgat
     1801 agcaatcgac cggataacga tgctccggcc ccgccgaggg cgcgatcagg aagagatagc
     1861 cgctcggccg caacacgcgc accatttccg cgaagctgag ccagaagaat tcgcaatgct
     1921 cgagcatctg gccggacagg acgatatcca tcgatcggtc gtcgaagggc aggcgatagg
     1981 gatcgtcgag gacgacatgg atgccgggcc cgtccgagat gtccgccgcc gtataggcaa
     2041 agcgcggatg gtcgaagatc tgccggtagc tgccgttgac gtcggcaccg ccgacatcga
     2101 gaatccgcac ctcctcctgc gtgccgagtg ggccacccgc cacgaagcgc gaatagcatt
     2161 tctgcatgtt ctcaaatgac gacgcgtgca tcgcttaccc gtaccttcac tggctagaac
     2221 ccctcacccg caggacgaca gcaaaaatca cccaaaaaca tcattgtaga gaatatccga
     2281 taggtgccgc cccgccggtg tccgctcaag ctggaccacc acgtcgatgg agcggtgcac
     2341 ataggtcacg acgtcctcgt aactcatgcg gatgccggtc tggagcacca gcagcgccaa
     2401 ctgatcgatg gcgcgtgacg gtgaatccgc gtggatcgtg ctcagcgatc ccggatgccc
     2461 cgtgttgacg gcgcggagga acgtctccgc ctcccgcccg cggatctcgc cgaggatgat
     2521 gcgatccggc cgcatccgca gcgaggcgat cagaagatcc tcggcgctca cggccgcttc
     2581 gctgagcgcg ctgcgggcac agagcagccc tacggcgttt tcatggacca ccttgagctc
     2641 gggcgtatcc tcgatgacca ccagccgctc gcgggccgga atttcgcgga gcagggcatt
     2701 gaggaaggtc gtcttgccgg ttgacg
//
LOCUS       DATGEM010000523         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_944397, whole genome shotgun sequence.
ACCESSION   DATGEM010000523 DATGEM010000000
VERSION     DATGEM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944397"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VKQ27_16035"
     CDS             complement(<1..928)
                     /locus_tag="VKQ27_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(338..340),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY90492.1"
                     /translation="MSDLTRRQFLKVTGMSLVGSSLAVLGFSPGPALAEVREYKLTRA
                     TETRNTCPYCSVACGILMYGLGDAAKNAQPSIFHIEGDPDHPVNRGTLCPKGAGLIDF
                     IHSESRLKYPEYRAPGSDRWQRMSWDDALDRIAKLMKQDRDANFVAKTPEGLTVNRWL
                     TTGMLAASASSNEVGYLTAKVARSLGLLAFDNQARVUHGPTVAGLAPTFGRGAMTNHW
                     VDIKNADVILIMGGNAAEAHPCGFKWVTEAKAHNKARLIVVDPRFTRSASVADYYAPI
                     RTGTDITFLGGVINYLLANDKIQHEYVKSYTDF"
     gene            complement(1091..1807)
                     /locus_tag="VKQ27_16040"
     CDS             complement(1091..1807)
                     /locus_tag="VKQ27_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HLY90493.1"
                     /translation="MQAYSRILRSLSTLALTLSLSLSPTPSSTGVARAAAAWTEGKNY
                     FLIDPPRTPSVAPGKVEVTEVFSYGCPACNLFVPVMHKLQKALPPNAVLDYLPASFNP
                     SEDWPMFQLAYLTAQTLGIADQSHDAMFDAVWTSRELSVIDPATQRIRERLPTIEDAA
                     RFYNRRSGVPVDKFVSTAKSFAVDVKVRSAEDAIKAYKVDRTPTIVVNGKYRLYQQSA
                     GSNDDLIELVNWLVAKETKH"
     gene            2141..3436
                     /locus_tag="VKQ27_16045"
     CDS             2141..3436
                     /locus_tag="VKQ27_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY90494.1"
                     /translation="MTDSSVSASGGPPGFIRRHRVALLVLATAAVLITVVVLFVRHRS
                     QAAPVPGAGRGGQNGPVAVAVATVTPGDIQIRIPAIGTVTTLANVTVRTQISGTLQKI
                     LFTEGQLVRSGDVLAQIDPRPYEAALQTAEGNLRRDQSLLANARLDLKRYEGLVKEDS
                     IAAQQLDTQRALVDQYAGTIESDEGQVKTAQVNLVFTRIVSPVSGRVGIRQVDQGNYV
                     TPGDTNGIVVVNQVQPISVIFSIPEDNVAALLKKVRQGATMKVEAYDRADAVKLAEGK
                     LATIDNSIDTTTGTVKLRAQFENADGALFPNQFVNVSLLLDLQQNQLIMPNAAVRRGA
                     PNGIVSTFVYRVNPDRTVSVRPVTLGTADGERVAVTSGVAAGDVVVTEGGDRLRDGAP
                     VQLPAAAASVASGASAAPAAPVAKRPPGAHRRPRTNNAQ"
     gene            3433..6540
                     /locus_tag="VKQ27_16050"
     CDS             3433..6540
                     /locus_tag="VKQ27_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HLY90495.1"
                     /translation="MNPSRIFILRPVATTLLMVAILIVGAIAYRQLPLSALPEVAYPT
                     IQVQTFYPGASPEVVTSAITAPLEKQLGTMPGLNQMSSSSSAGASVITLQFNLALPID
                     VAEQEVQASISGAQNLLPQDLPAPPIYAKVNPADAPVLTLGITSKVMPLTEVEDMADT
                     RIAQKLSQLKGVGVVSISGGQRPAVRVVVNPRALAAYGLNIDDLRTTINNANQNGPKG
                     TLDGPVNAYTVNTNDQIRNADDYGDVVVAYKNGAPVRLRDVATLVAGAENAKLGGWMN
                     SMPALIVNVQRQPGANVVDVVDRVHELLPTLQAAMPPGVDVAILTDRTTTIRASVRDV
                     EFELGLAVVLVVLVIYVFLRNVPATIIPSLSVPLSLIGTFAVMYMANFSLNNLSLMAL
                     TIATGFVVDDAIVMIENISRYIEAGESPLQAALKGAGQIGFTIISLTVSLIAVLIPLI
                     FMQDVVGRLFREFAITLAVTILISAVVSLTLVPMLCAKLLERRDEVAGARESGRWFAA
                     VVEWYGRRLAWVLDHQRLTMWVATGTVAVTALLYILIPKGFFPEQDTGLIQGVSEAGQ
                     NISYRAMADRQQALAVAILTDPDVQSLSSFIGVDGNNVTLNSGRFLINLKPRDQRTSA
                     VADVIRRLRSEIADVSGITLFMQPVQDLTLDNTVSRTQYQFTLESADPAALSAWTPKL
                     VSILRRQSELADVVSNEEDNGLAAYVTIDRDSAARLGISVGTVDNALYDAFGQRIVST
                     IFTQSNQYRVILEADPHSYRTVDSLASIYVPAAGGGQVPLSAIVKVDVETRPLLINHL
                     AQFPATTISFNLAEGTSLGAAVAAIEKAERGAGLPNSIRTTFQGAALAFRSNLDNELL
                     LLLAAVAVMYIVLGILYESFIHPVTILSTLPSAGIGALLALILAGDDLTIIALIGIIL
                     LIGIVKKNAILMIDFAIDAQRDDGLSPREAIFQACLLRFRPILMTTVAAIFGALPLMF
                     GTGTGSELRHPLGISIVGGLLVSQVLTLFTTPVIYLAFDRIAARFRGRPPRDAGDAAL
                     ADNPT"
     gene            6537..>9030
                     /locus_tag="VKQ27_16055"
     CDS             6537..>9030
                     /locus_tag="VKQ27_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90496.1"
                     /translation="MNISALFIRRPVATTLLTVGVALCGAVAFMLLPVAPLPRIDIPA
                     IFVSAALPGASPEVMASTVATPLERHLGAIADVDDMTSTSGVGTSNIQLTFGVDRDID
                     GAARDVQAAIVAAHADLPSALTRNPSYRKINSSLFPVMAIAMTSDVLTQGQIYDAADA
                     VISQRLSQIQGVGGVNVNGSALPAVRVEINPQSLSKYGIGLQDVRAAISNANANAPKG
                     AIESSDLRYQIYTNDNARDAEPYRNLIIANRNGSVVRLGDVAEVLDMQDGATEDIRTY
                     GLYNGKPAVFVTVYQQPGANVIEMIDAVKAELPLLKGSIDPKIDLVVTFDRSVTIRAS
                     LRQVEQTLMLAVAMVILVVYIFLNSHRAALVPALVVPVSLIGTFGAMYLLGYTLDNFS
                     LMALTVATGFVVDDAIVVMENTTRHIEGGMDRMRAALLGAREVGFTVVSMSLSLIAVF
                     IPFMFAGGIVGKIFREFTVTLSVAILISLVISLTTTPMMCSRLLERHERRKPSRFAAA
                     FDRWFERLRGGYERSLQWALHHRRIMMLLLGATVVLNGYLYFAIPKGFFPQQDTGQLQ
                     GGIRGDAASSFQFMKRKLQEVAAIIQQDPAVSTVTGSVGAGGFGRGGGGAAGNLTIAL
                     KPLAERRISADQVIARLRPTLGRVEGVAAYLQAVQDIGGGGGRSANAQYQYTLLGDDL
                     GELRSWSQRLRGALQDVPELTDVDSDLQPGGLEANLIVDRDAASRLGLTEVQIDNALG
                     DAFAQAQVSTIYNPFSPQQYHVVMEVAPRYWQNPDVLKELYISTGGAAVSGTQATQAV
                     AGTTVLTGTTRTSATAVAQDAARNQAANSLANA"
BASE COUNT         1595 a         2999 c         2810 g         1626 t
ORIGIN      
        1 tgaagtccgt gtagctcttc acatactcgt gctggatctt gtcattcgcg agcaggtagt
       61 tgatcacccc gcccaggaag gtgatgtccg tgccggtccg gatcggcgcg tagtagtccg
      121 cgacggacgc cgagcgcgta aaacggggat cgacgacgat cagccgcgcc ttgttgtgcg
      181 ccttggcttc agtgacccac ttgaatccgc acgggtgcgc ctcggcggca ttgccaccca
      241 ttatgagaat gacgtcggca tttttgatgt cgacccagtg attcgtcatc gctccacggc
      301 caaacgtcgg ggcaagacct gccaccgtcg ggccgtgtca cacacgcgcc tggttgtcga
      361 atgccagcaa acccagggag cgcgccacct tggcagtcag gtagccgact tcgttgctcg
      421 aagcggatgc cgccagcatt cccgtcgtca gccaccggtt gacggtcaat ccctccgggg
      481 tcttggcgac gaagttcgca tcgcggtcct gtttcatcag tttggcgatt cgatcgagag
      541 cgtcgtccca ggacatccgc tgccatcgat ccgatccggg tgcgcgatac tcgggatact
      601 tgagacggct ctcgctgtgg atgaagtcga tcaggccggc tcccttcggg catagcgtgc
      661 cgcgattgac cggatgatcg gggtctccct cgatatgaaa gatgctcggc tgcgcattct
      721 tcgccgcgtc gccgagaccg tacatgagaa taccgcaggc aaccgaacaa taggggcacg
      781 tattgcgcgt ctcggtagcg cgcgtgagct tgtattcacg cacctccgcg agcgccggcc
      841 cgggtgagaa acccaagacc gccagactcg aacccaccag cgacatgccg gtgaccttca
      901 agaactggcg cctagtcaag tcggacatga catcctcctc gcgacgaccg ccgtaaaacc
      961 gcaagacagc aaaccgcaac aaggcagtca gccgagttta ggcccgcggc gagggctcga
     1021 caagttgcgg tcttgggcgg cgaccgcagc ccgggcgcag atcgaatgcg cgcgcggcgc
     1081 gcccgcgggg tcagtgcttg gtttctttgg ccaccagcca gttcaccagc tcgatcaagt
     1141 cgtcgttgct gccggcggat tgctggtaca gccggtattt cccgttcacg acgatggtcg
     1201 gcgtccggtc gaccttgtac gccttgatgg cgtcctcggc gctgcgcacc ttgacgtcca
     1261 cggcgaacga tttcgcggtg ctcacgaatt tatcgacggg cacgccggag cggcggttgt
     1321 agaagcgcgc cgcgtcctcg atcgtcggca gacgttcccg gatgcgctga gtggcgggat
     1381 cgatcacgct caattcgcgg ctggtccaca ccgcatcgaa catggcatcg tgggactgat
     1441 cggcgatacc cagcgtttgc gcggtcaggt acgcaagctg gaacatcggc caatcctcgc
     1501 tgggattgaa cgaggccggg aggtagtcga gcaccgcgtt gggcggcagc gctttctgca
     1561 gcttgtgcat caccggaacg aacagattgc acgccggaca cccgtatgaa aacacctcgg
     1621 tgacttcgac ttttcccgga gcgaccgagg gagttcgcgg tggatcgatg aggaagtaat
     1681 tcttcccctc ggtccaggcg gcggccgctc gcgcgacgcc cgtcgatgac ggcgtcggcg
     1741 acaacgacag cgacaaggtc agggcaaggg tcgagaggct gcgaagaata cgggaatatg
     1801 cctgcattgc gactccatcc gatgtgcgat gccaatgatg catcaagacc aagtctaacc
     1861 gattcgttgc gaggcaagcc gcaccatcgc cccgggggaa gtgaccggaa cgatcgcagg
     1921 atcgcatggc aaccgattcg ttacggcaac cgcaaccgtc gcccggggga cgtggccgga
     1981 acgatcgcag gatcgcatgg ccttgacgct tcctcacact aattggcaca tgtccgccgc
     2041 gttcggtagt cccgctggcg ggaacaccgt aaagtggcgg gtgatttatg atatcgggtt
     2101 gtcggaccgg gtgaacatgt ggtaaacgag agcgatagca gtgaccgata gcagtgtgtc
     2161 cgcatccggc ggcccgccgg gtttcatccg caggcaccgg gtcgcgctgc tcgtgctcgc
     2221 cacggcggcc gttctgatca cggtcgtcgt cctgttcgtg cggcaccgct cgcaggcggc
     2281 gcccgtccct ggcgccggac gcggcggcca gaacgggccg gtggcggtgg ccgttgcaac
     2341 ggtgacgccc ggcgacattc agatccggat tcctgcgatc ggcacggtga ccacgttggc
     2401 caacgtgacc gtacgcacgc agatcagcgg taccctgcag aagatattgt tcacggaagg
     2461 gcaacttgtc cgttccggcg atgtccttgc tcagatcgat cctcgaccct acgaagcggc
     2521 gctgcagacg gccgagggca atctgcgccg cgaccaatcg ctgctcgcga atgcccggct
     2581 cgacctcaag cgctacgagg gtctggtcaa ggaggactcg atcgccgcgc agcagctcga
     2641 cacccagcgt gcgctggtgg atcagtacgc gggaacgatc gaatcggacg agggccaggt
     2701 caagaccgcc caggtgaacc tggtgtttac gcgcatcgtc tcgccggttt ccggccgggt
     2761 cgggatacgc caggtggacc agggcaacta tgtcactccg ggcgacacga acggcatcgt
     2821 ggtggtgaac caagtgcagc ccatcagcgt gatattttcc attcccgaag acaacgtcgc
     2881 ggcgctgctg aagaaggtgc gtcagggagc cacgatgaag gtcgaggctt acgaccgcgc
     2941 ggatgcggtg aagctcgccg agggcaagct cgcgaccatc gacaacagca tcgacaccac
     3001 cacgggaacc gtcaagctgc gcgcacaatt cgagaacgcc gacggcgcgt tgttcccgaa
     3061 tcaattcgtc aacgtatccc tgctgctcga tttgcagcag aaccagctca tcatgccgaa
     3121 cgccgcggtg cgccgcggcg cccccaacgg catcgtctcc acgttcgtat atcgcgtgaa
     3181 tccggaccgg acggtgagcg tgcggccggt gacgctcggc accgccgatg gcgagcgcgt
     3241 ggccgtcacc tccggcgtcg cggccggcga tgtcgtggtc accgagggag gcgatcgtct
     3301 gcgggacggc gcgccggtcc agctgccggc tgcggcggcc agtgtggctt ccggggcctc
     3361 ggcggcaccc gcggcgcccg tcgccaaacg gcccccgggc gcccaccgcc gcccgcggac
     3421 caacaacgca cagtgaatcc gtcgcgcatt ttcattctgc gtcccgtggc gaccaccctg
     3481 ttgatggtcg ccattctgat cgtgggcgcc atcgcctacc ggcaattgcc cctgtcggcc
     3541 ctgccggaag tggcctaccc cacgatccag gtgcagacct tctatcccgg tgcaagcccc
     3601 gaagtcgtga cctccgccat cacggcgccc cttgaaaagc agctcggaac catgcccggc
     3661 ctgaatcaga tgtcctcgag cagttccgcc ggcgcctcgg tcatcacgct ccagttcaat
     3721 ttggcgctcc ccatcgacgt cgccgaacag gaggtccagg cctccatcag cggcgcccag
     3781 aatctgctgc cccaggatct gccggcaccg ccgatctacg ccaaggtcaa ccccgccgac
     3841 gcgccggtgc tgaccttggg catcacctcc aaggtcatgc ctctcacgga agtcgaggac
     3901 atggcggaca cgcgcatcgc ccagaaactc tcgcagctca aaggggtggg cgtggtcagc
     3961 atcagcggcg gacagcggcc cgccgtcaga gtggtcgtga atccgcgggc cctcgcggca
     4021 tacggattga atatcgatga tttgcgcacg acgatcaaca acgccaacca gaacgggccg
     4081 aagggtaccc tcgacgggcc cgtcaacgca tacaccgtca acaccaacga ccagatcaga
     4141 aacgccgacg actacggcga tgtcgtggtc gcgtacaaga acggcgctcc ggtacgtctg
     4201 cgggatgtgg ccaccctggt cgccggcgcc gagaacgcca agctcggcgg ctggatgaac
     4261 tcgatgccgg cattgatcgt caacgtgcag cgccagccgg gagccaacgt cgtcgacgtg
     4321 gtcgaccggg tccacgaact gctgccgacc ctgcaggcgg ccatgcctcc cggggtggat
     4381 gtcgccatcc tgacggaccg caccaccacc atcagagcct cggtgaggga cgtcgaattc
     4441 gagctcgggc tggcggtggt gctggtggtg ctggtcattt atgtgttcct ccgcaacgtg
     4501 ccggcgacca tcattccgag cctgtcggtg ccgctgtcgc tcatcggcac cttcgccgtg
     4561 atgtacatgg ccaatttcag cctcaataac ttgtccctga tggccctgac catcgccacg
     4621 ggattcgtgg tggacgacgc catcgtcatg atcgaaaaca tctcgcgcta catcgaagcg
     4681 ggcgaatccc ccttgcaggc cgcgctcaag ggcgccggac aaatcggctt caccatcatc
     4741 tcgctgacgg tctcgctgat cgcggtgctg atcccgctca tcttcatgca ggacgtggtg
     4801 ggccgtctgt ttcgggaatt cgcgatcacg ctggccgtca ccatcttgat ctcggcggtg
     4861 gtgtccctga cgctcgtgcc catgctgtgc gcaaaactcc tcgagcgccg cgacgaggtc
     4921 gccggcgctc gggaatccgg ccgctggttc gccgccgtgg tcgaatggta cggccgcagg
     4981 ctcgcctggg tgctcgatca ccaacggctc acgatgtggg tcgcgaccgg caccgtcgcc
     5041 gtgacggcgc tcctgtacat cttgatcccg aagggattct ttccggaaca agacaccggt
     5101 ttgattcaag gcgtgtcgga agcgggccag aacatctcct accgcgccat ggccgatcgc
     5161 cagcaggcgc tcgccgtcgc cattctcacc gatcccgatg tccagagcct ttcctccttc
     5221 atcggcgtcg acggcaacaa tgtcaccttg aacagcggcc gttttctcat caatctgaag
     5281 ccccgcgatc agcgcacctc tgcggtcgcc gacgtgatcc gccgccttcg ttccgaaata
     5341 gccgacgtct ccggcatcac gctcttcatg caacctgtgc aggacctgac gctcgacaac
     5401 acggtcagcc gcacgcaata tcaattcacc ttggagagcg ccgatcctgc ggccttgtcg
     5461 gcctggacgc ccaagctcgt gtcgattctg cgccggcagt ccgaactcgc cgatgtggtc
     5521 agcaacgaag aagataatgg actggcggcc tacgtcacca tcgatcggga cagcgccgcg
     5581 cgactcggga tcagcgtggg cacggtggac aacgcgctct acgatgcctt cgggcagcgc
     5641 atcgtctcca ccatcttcac ccaatcgaat caatatcggg tgattctgga ggccgatccg
     5701 cactcgtacc ggaccgtcga ctcgctcgcg tcgatttacg tgccagccgc cggcggcggg
     5761 caggtgcctc tgtcggcgat cgtcaaagtc gacgtggaaa cgcggccgtt gctcatcaat
     5821 catctggcgc agtttcccgc gaccaccata tcgttcaatc tggccgaggg cacctcgctc
     5881 ggcgccgcgg tggcggccat cgagaaggcg gaacgcggcg ccggtctgcc gaacagcatc
     5941 cgcaccacgt ttcagggcgc cgccctcgcg tttcgcagca atctcgacaa cgaactgctg
     6001 ctgttgctgg ccgcggtggc cgtcatgtac atcgtgctcg gcattctcta cgaaagcttc
     6061 atccatcccg tcacgatcct gtcgacattg ccgtccgccg gcatcggcgc gctgcttgcc
     6121 ttgatcctcg ccggcgacga tctcaccatc atcgcgctga tcggcatcat cctgctgatc
     6181 ggcatcgtca agaagaacgc gattctcatg atcgatttcg cgatcgatgc ccagcgcgac
     6241 gacggcctct cgccgcgcga ggccatcttc caggcatgtc tgctgcgctt ccgcccgatc
     6301 ttgatgacca ccgtggccgc gatattcggg gccttgccct tgatgttcgg cacgggaacc
     6361 ggctccgaat tgcggcaccc gctcggcatc agcatcgtgg gaggactgct ggtgagccag
     6421 gtgctgacct tgttcaccac gcccgtgatc tatctcgcat tcgaccggat cgccgcgcgg
     6481 tttcgcggcc ggcctccccg cgacgcgggc gacgccgccc tggcggacaa ccccacgtga
     6541 acatctccgc gctcttcatc cgcagacccg tcgcaaccac gctgttgacg gtgggcgtcg
     6601 cgctgtgcgg cgccgtggcc ttcatgctgc tgcccgtggc gccgttgccg cgcatcgaca
     6661 tcccggccat tttcgtctcc gccgcgctgc cgggcgcgag ccccgaggtc atggcgagca
     6721 cggtcgcgac gcccttggag cgccatctcg gggcgatcgc ggacgtcgac gacatgacct
     6781 cgaccagcgg cgtcggcact tcgaacatcc agctgacgtt cggcgtggac cgggacatcg
     6841 acggcgccgc gcgcgacgtg caggcggcca tcgtcgcggc gcacgccgat ctgccctccg
     6901 cgctcacccg caatccgtcg taccgcaaga tcaacagttc gctgtttccg gtgatggcca
     6961 tcgcaatgac gtccgacgtg ctcacccagg gacagattta cgacgcggcg gacgcggtga
     7021 tttcccagcg attgtcgcag atccaggggg tgggcggagt caacgtcaac ggcagcgcgc
     7081 tgccggcggt ccgggtggag atcaatcccc agtccctgtc caagtacggc atcggcctgc
     7141 aggacgtgcg ggcggcgatt tccaatgcca acgccaatgc gccgaaaggc gcgatcgagt
     7201 cctccgatct gcgctaccag atctatacca acgacaacgc gcgcgacgcc gagccgtacc
     7261 gcaatctcat catcgccaac cgcaacggct cggtggtgcg cctcggcgac gtcgcggagg
     7321 tcctcgacat gcaggacggc gccacggagg acatccgcac ctacggcctg tacaacggca
     7381 aaccggcggt cttcgtgacc gtgtaccagc agcccggcgc gaacgtcatc gaaatgatcg
     7441 atgccgtgaa ggcggagctg ccgctgctca agggttcgat cgatccgaag atcgatctgg
     7501 tggtcacctt cgaccgttcg gtcacgatcc gcgcgtcgct gcgccaggtg gaacagaccc
     7561 tgatgctggc cgtcgccatg gtgattctcg tcgtctacat cttcttgaac agccatcgcg
     7621 cggcgttggt gccggccctg gtggtgcccg tctccttgat cggcacgttc ggcgccatgt
     7681 acctcttggg ctacaccctc gacaattttt cgctgatggc attgaccgtg gccaccggtt
     7741 tcgtcgtgga cgatgccatc gtcgtgatgg agaacaccac gcggcacatc gagggcggga
     7801 tggaccgcat gcgcgccgcg ctcctgggcg cccgcgaggt ggggttcacc gtggtttcca
     7861 tgagcctgtc gctgatcgcc gtgttcattc ccttcatgtt cgccggcggg atcgtgggca
     7921 agatcttccg cgagttcacg gtcaccctgt ccgtcgcgat tctgatctct ctggtgattt
     7981 cgttgaccac cactcccatg atgtgctcgc ggctgctgga gcggcatgaa cggcgcaaac
     8041 cgtcgcgctt cgcggccgcg ttcgaccgct ggttcgagag gctgcgcggc ggctacgaac
     8101 ggagtctgca gtgggcgctg catcaccggc gcatcatgat gctgctgctc ggcgccaccg
     8161 tcgtgctgaa cggctatctc tacttcgcca tccccaaggg cttcttcccg cagcaggaca
     8221 ccggccagtt gcagggaggc atccggggcg atgcggccag ttccttccag ttcatgaagc
     8281 gcaagctcca ggaggtcgcg gcaatcattc agcaggatcc cgcggtcagc acggtgaccg
     8341 gctcggtggg agccggcggc ttcggacggg gtggcggcgg cgcagccggc aacctgacca
     8401 tcgcactgaa gccgttggct gaacggcgca tctccgccga tcaagtcatt gcccgattgc
     8461 ggccgacgct cggcagggtc gaaggcgtcg ccgcctactt gcaagccgtc caggacatcg
     8521 gcggcggcgg cggccgttcc gccaatgccc agtatcaata cacgctgctt ggcgacgatc
     8581 tcggcgagct tcgtagctgg tcgcaacgtt tgcggggcgc gcttcaggac gtacccgaac
     8641 tgaccgatgt ggactccgat ctgcagcccg gcggtctcga ggcgaatctc atcgtcgacc
     8701 gcgacgccgc ctcccggttg ggcttgaccg aagtgcagat cgacaacgcg ctgggcgatg
     8761 ccttcgcgca agcccaggta tcgaccatct acaatccatt cagcccgcag cagtaccacg
     8821 tggtgatgga ggtggcgcct cggtactggc aaaacccgga cgtcctcaag gaactctaca
     8881 tcagcaccgg aggcgccgcg gtcagcggca cgcaagcgac gcaggccgtg gccggcacca
     8941 ccgtgctcac gggaacgacc cgcacgagcg ccaccgcggt ggcgcaggat gcggcccgca
     9001 accaggcggc gaactcgctg gccaatgcgg
//
LOCUS       DATGEM010000524         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_146084, whole genome shotgun sequence.
ACCESSION   DATGEM010000524 DATGEM010000000
VERSION     DATGEM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146084"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VKQ27_16060"
     CDS             <1..1262
                     /locus_tag="VKQ27_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLY90497.1"
                     /translation="QRDIIMWMDHRAIAEAADINATGDTALDYVGGEVSVEMELPKVL
                     WLQRHLPQRHAMVHRYFDLADYLVWRATGTDVASVCTLTCKWNYLAHENRFAGSLLRD
                     AGLRDLLEKVPAKVLPLGSAAGMLHRRCAAELGLSTTTVVATGIIDAHAGGLALLAAA
                     PEGGLAIVSGTSCCHLVVNRRRIMVPGVWGPYFGAMLPGWWLNEGGQSAAGSLIDWTL
                     RQSDAWAEMQASGRNIYEVANEWVAGLEARETDPTRHLHVLADHHGNRSPRSDPLSRG
                     LIAGLSLERGPEGLARRYLATVQALAYGTRHIVETLNAAGHRIERVLITGGGIRNALS
                     LREHADAIGCDLHLGSEDQEVTRGSALLAATASGAYPSLPAAAAAMVRPGNVVRANQA
                     RRAFHDAKYTVYLSLYDDWRRYRQMMR"
     gene            1266..2915
                     /locus_tag="VKQ27_16065"
     CDS             1266..2915
                     /locus_tag="VKQ27_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90498.1"
                     /translation="MLSLNLVEAAPLIRVGANEVLLVTNADLRESANVVCWPVQAKFE
                     HKLQEVLRDRFGTSVKRAHPIKADKGHGFISSQREGSDLFEHIDPHAPIIVLLTAWQY
                     SHHLAPSLVHHRGPILLLANFDGTWPGLVGMLNMAGTLNSLGRHCSRLWSEDFDDAFF
                     LRSLGEWLTSGFIEHDTDYLHPIAPDHPVMHSIAGQIGKRVGEHVLRHKDIMGLFDTF
                     CMGMINGVFPQQALIDIGMPLEGLSQSALLAEMAKVPRDLCEACLSWYEARGMRFSFG
                     LEPTTELTREQVLEQCAMLIALGRFAQRFGLSAVGVQYQQGLAQSCAASDFAEGAIGS
                     AERFPIPDEVGAIVRAGKPIPCVNEVDMGSGIPQTMLWRLLDSLGLPSETTLHDIRWG
                     SDYQGTFYWDLEISGAVPFGHLKGGIAGATGYRQPPMYFPHGGATIAGQCKGGRFIWA
                     RAHYVGTQVFMHLGTGRAVELPPDEFERRRHGTTYEWPLMNVVLDGVRRDDLMAGHQS
                     NHITVAYVGEAQLRDVFRAFVAQAVTQNIATSVAGSPIGWL"
     gene            complement(2925..>3915)
                     /locus_tag="VKQ27_16070"
     CDS             complement(2925..>3915)
                     /locus_tag="VKQ27_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90499.1"
                     /translation="RFLIGGALLMSLCITAMGFTTAFWQILVLAPLSGLGNSVFHPCD
                     YAILAGSVDKDRMGRAFALHTFSGNLGFAAAPPVMAVLMVAFGWRVSLMLVGLLGVLV
                     AISIVLQSSILNDQVRQEPTHAGSAMSGSELLMSRNMLLFFGFFLFGSMAGAGVQSWL
                     ITVLHTLKGFTLEMASAALTGYMAGSTFGVLVGGWFADTYKRHVLAFAVILTIVSAAL
                     IVAVDWLPLSALAAIGVMFASGLALGASRTPRDVMLKDAAPPGQIGKVFGFVSAGLPL
                     GGALTPVPFGFLIDRGHPELILTLAAVLLLASLLCVGTARSTARPTPAAVAAE"
BASE COUNT          725 a         1214 c         1287 g          689 t
ORIGIN      
        1 cgcagcgcga catcatcatg tggatggacc accgcgccat cgccgaggca gcggacatca
       61 atgcgacggg tgatacggcg ctcgactacg tcggaggcga agtcagcgtc gagatggaat
      121 tgccgaaggt gctgtggttg cagcgccacc tgccgcagcg ccatgccatg gttcatcgct
      181 acttcgacct ggcggattat ctggtctggc gcgcaaccgg taccgatgtg gccagcgtct
      241 gcacgttgac ctgtaagtgg aactacctcg cgcatgaaaa tcgcttcgcg ggcagcctgc
      301 ttcgggatgc gggcctgcgg gacctcctgg agaaggtgcc ggccaaggtc ctgccactcg
      361 gttccgctgc cggcatgctg cataggcgtt gtgccgccga gcttggactg tccaccacca
      421 ccgtggtggc gacaggaatc atcgatgccc atgcaggcgg actggcattg cttgccgcgg
      481 cccctgaagg cggtctcgcg attgtcagcg gcacctcgtg ctgccatctc gtggtcaacc
      541 ggcggaggat catggttccc ggcgtctggg gcccatattt tggtgccatg ctgcccgggt
      601 ggtggctcaa cgaaggtggc cagagtgcgg ccggctcgct gatcgattgg acgttgcggc
      661 agagcgatgc ttgggccgag atgcaggcaa gtggccgcaa catttacgag gtggccaacg
      721 agtgggtcgc agggctggaa gcgcgcgaaa ctgatccgac acgccatctg catgtgctgg
      781 cggatcatca cggcaatcgt tcgcctcgtt ccgatccatt gtcccgcggc ctgattgcgg
      841 gactgagcct tgagcgtgga ccggaggggc tggcccggcg ctatctggca actgtgcagg
      901 cgctggccta tggcacgcgc cacatcgttg agacgctgaa cgcggcgggg catcgcatcg
      961 agcgcgtact gatcaccggc ggtggcatcc gcaatgcatt gtcgttgcgc gagcatgctg
     1021 acgcgatcgg ctgcgatctg catctcggta gcgaggatca ggaagtgact cgcggttcgg
     1081 ccctgctcgc ggctaccgca agtggagcgt atcccagcct tccggcggcg gcggccgcga
     1141 tggtgcgccc aggcaacgtc gtgcgcgcca atcaagcgcg gcgtgctttc catgatgcca
     1201 aatacaccgt ctacctctcg ctctacgacg actggcgccg ctaccgacag atgatgaggt
     1261 gacccatgct ctcgctcaac cttgtcgaag cagcaccact gatccgtgtc ggtgccaatg
     1321 aggtgctgct tgtcaccaac gcagacctgc gcgagtcagc caatgttgtg tgctggccgg
     1381 tgcaggcgaa gttcgaacac aagctgcaag aggtcctccg cgaccggttc ggaaccagcg
     1441 tcaaacgcgc gcaccccatc aaggccgaca agggccatgg cttcatcagc agccaacgcg
     1501 aaggcagcga tctgttcgaa cacatcgatc cgcatgcgcc gatcatcgtg ttgctgaccg
     1561 cctggcaata ttcgcatcat ctggcgccca gccttgtgca ccatcgcggc ccgatcctgt
     1621 tgctggcgaa tttcgatggt acgtggccgg gacttgtcgg catgttgaac atggcgggaa
     1681 cgctgaacag cctgggcagg cattgctcgc ggctgtggtc agaggatttc gacgatgcgt
     1741 tcttcctgcg cagtctaggc gagtggctga ccagcggctt catcgagcac gatacagact
     1801 acctgcatcc aatcgcgccc gatcacccgg ttatgcactc gatagccggt caaatcggca
     1861 agcgtgtcgg cgagcacgtg ctgcggcaca aggacatcat gggtctgttc gacacgttct
     1921 gcatgggcat gatcaacggc gtgtttccac aacaggcgct gatcgacatc ggcatgccgc
     1981 tggagggttt gtcgcaatcc gcgttgctgg cggagatggc gaaggtgccg cgcgacctgt
     2041 gcgaggcttg cctgagctgg tatgaggcgc gcggcatgcg cttctcattc ggtctcgaac
     2101 cgaccaccga gctgacgcgc gagcaggtgc tggaacaatg cgcgatgctg atcgcgctcg
     2161 gccgcttcgc gcagcgcttc ggtttgtccg ccgtcggggt gcaataccag caggggcttg
     2221 cgcagtcctg tgccgcgtcc gacttcgctg aaggtgcgat cggttcggcc gagcgcttcc
     2281 caattcccga tgaggtcggc gccatcgtac gagcaggcaa gccgatcccg tgcgtcaatg
     2341 aagtcgatat gggcagcggt atcccgcaga cgatgttgtg gcggctattg gactcgctcg
     2401 gcctgccatc agagacgaca ttgcacgaca tccgttgggg cagcgactat cagggcacat
     2461 tttattggga cctggagata tcgggtgcgg tgccgttcgg ccatctgaag ggtggtatcg
     2521 ccggcgcgac gggctatcgg cagccgccaa tgtattttcc gcatggcggt gcgacgatcg
     2581 ccgggcaatg caaggggggg cgcttcatct gggcgcgcgc gcattacgtg ggcacgcagg
     2641 tgttcatgca tctgggcacc gggcgagccg ttgagctgcc accggatgag ttcgaacggc
     2701 gccggcatgg caccacttac gaatggccgc tgatgaatgt ggtgctcgat ggagtgaggc
     2761 gcgacgacct catggcgggt caccagagca accacatcac agtcgcctac gtgggcgaag
     2821 cgcagcttcg ggacgtcttc cgcgccttcg tcgcacaggc ggtcacgcag aacatcgcca
     2881 catcggtcgc cgggtcgccg ataggctggc tgtagtcgct ccggctattc ggccgccacg
     2941 gcggccggtg tgggccgtgc tgtggaccgc gcggtgccga cgcacagcag gcttgcaagc
     3001 agcagcacag ccgccagcgt caggatgagt tccggatgcc cgcggtcgat caggaagccg
     3061 aatggcaccg gcgtcagcgc gccaccaagc ggcagccctg cactgacgaa gccgaatacc
     3121 ttgccaatct gtccaggtgg tgctgcgtcc ttgagcatca cgtcgcgcgg cgtgcggctc
     3181 gcccccagcg ccaggcccga cgcgaacatc accccgatcg cggccagggc cgacagcggc
     3241 agccaatcca ccgcaacgat cagtgcggcc gatacaatcg tgaggatcac cgcgaaggcc
     3301 agaacatggc gcttgtaggt gtcggcgaac cagccgccca ccagcacgcc aaaggtggac
     3361 cccgccatgt acccggtgag cgcggctgag gccatctcca acgtgaagcc cttcagcgtg
     3421 tggagcaccg tgatcagcca cgactgcact ccggcgcccg ccatcgagcc gaacaggaag
     3481 aagccgaaga acaacagcat gttgcggctc atgagcagct cgctgccgga catcgccgag
     3541 cccgcatgag tcggttcttg tcgcacctgg tcgttcagga tcgagctctg cagcacgatg
     3601 gagatcgcga ccaggacgcc aaggagcccg acaagcatca ggctgacgcg ccagccgaag
     3661 gcgaccatca gcactgccat caccggaggc gcggccgcaa agcccaggtt gccgctgaac
     3721 gtgtgcaggg cgaaagcgcg gcccatgcgg tccttgtcca ccgacccggc gaggatcgcg
     3781 tagtcgcacg gatggaacac cgagttgccg agacccgata gcggcgccag caccaggatt
     3841 tgccagaagg cggtggtgaa gcccatggca gtgatgcaca gcgacatcag caatgcaccg
     3901 ccgatcagga agcgg
//
LOCUS       DATGEM010000525         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_31219, whole genome shotgun sequence.
ACCESSION   DATGEM010000525 DATGEM010000000
VERSION     DATGEM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31219"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2572)
                     /gene="pepN"
                     /locus_tag="VKQ27_16075"
     CDS             complement(<1..2572)
                     /gene="pepN"
                     /locus_tag="VKQ27_16075"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HLY90500.1"
                     /translation="MSQTAPHETRLADYRPPAYLVDTADLAFDLDATATKVRSRLSVR
                     RNPESQGTGALLLDGEALKLVRIALDGVELGANRYRIDDGKLLIPDMPDTAVLEIETQ
                     IAPKDNTELSGLYTSNGSYFTQCEAEGFRRITYFPDRPDVMARFTVSITADKARVPVM
                     LSNGNPSAVEDAGGGRHRIIWTDPHPKPCYLFALVAGDLVAVKDSFTTRSGRDVQLGV
                     WVRRGDEDRCAHALRSLKTSMKWDEDVFGLEYDLDVFNIAAVSDFNMGAMENKGLNVF
                     NTKYVLAKPETATDNDYQGIESVIAHEYFHNWTGNRVTCRDWFQLSLKEGLTVYRDQE
                     FSADQGSRVVKRIGDVRALRAAQFREDAGPLAHPVQPDHYITIDNFYTATVYNKGAEV
                     IRMMATIIGREAFRRGMDLYFQRHDNHAVTINDFAQAMQDASGVDLTGFRRWYHQAGT
                     PQVTVSTAHDAAARRYTLTLKQHTAPTPGEPEKQPLVIPVVMGLLDDAGHEIASRTLL
                     LSEPEQSFAFDGIDQPPVPSLLRRFSAPVKLSGLPLAQLQFLAAHDTDPFVRWESGQQ
                     YATQLLLDMAAVWRHGETPAVTTGLIDALAATLRDGDADPAFAAEALMLPSESFLADQ
                     MAVVDVDAIHAVRNMARVAVGAALAAELRATYDRLTDTGPYRIDGDSIGRRALRNACL
                     AYLAADGNAAGVARAKAQFDAGQNMTDVLAAMATLSAVDCPERRAALDAFHARWRGDD
                     LVLDKWFAIQAMSPLPDTLDAVRALSRHADFDLRNPNRMRALIASFASGNQVRFHDAS
                     GAGYRFLSDTIITLDPMNSQVAARLVPPLGQWRRFDEARQALMKQELQRILDMPNLSK
                     G"
     gene            complement(2595..>3095)
                     /locus_tag="VKQ27_16080"
     CDS             complement(2595..>3095)
                     /locus_tag="VKQ27_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90501.1"
                     /translation="RQGARRAHGAVAPRTDRRGAITATAAMRHLAIAASLCLAGAAVA
                     QPAPFDALAASSAIVNNIAHQDLDAAAEAAARLMESTPADRLRGTFRFVKDLGAAQYT
                     DLVYARDYGRSGKDVIYKIDFAKNFLFVRLLYQVDNGAWHLIHVTLKTEDELPFPRDW
                     THIYPK"
BASE COUNT          570 a          979 c          981 g          565 t
ORIGIN      
        1 tgcccttgct gagattaggc atgtcgagaa tgcgctgcag ttcttgcttc atcagcgcct
       61 gccgcgcctc atcgaagcgg cgccattgac caagcggcgg caccagccgc gcggccacct
      121 ggctgttcat cggatccagc gtgatgatgg tgtccgacag gaagcggtag ccggcaccgg
      181 atgcatcatg gaaccgcacc tgattgcctg atgcaaaact agcgatcagc gcgcgcatgc
      241 ggttggggtt gcgcagatcg aagtcggcat gccgcgacaa ggcgcgcacc gcatcgagtg
      301 tatccggcaa tggcgacatc gcctgaattg caaaccactt gtccagcaca aggtcatcgc
      361 cccgccagcg cgcatggaat gcatcgagcg cggcgcggcg ctcgggacaa tcgaccgccg
      421 acagcgtggc catcgcggcg agcacatccg tcatgttctg tcccgcgtcg aactgcgcct
      481 tggcacgcgc gacgcccgcc gcgttgccat ccgccgccag ataggcgagg caggcattgc
      541 gcaatgcacg ccgaccgatc gagtcaccgt cgatgcgata ggggccagta tcggtcagcc
      601 gatcgtacgt agcgcgcagt tcggcggcca gcgcggcgcc aaccgcgacc cgcgccatat
      661 tccgcacggc atggatcgca tcgacatcca caaccgccat ctggtcggcc aagaagcttt
      721 cgctcggcaa catcaatgct tcggcagcga aggccggatc ggcatcgcca tcgcgcagcg
      781 ttgcggcgag tgcgtcgatc agcccggtgg tcaccgccgg tgtttcgcca tgtcgccaaa
      841 cggccgccat atccagcagc agttgcgtcg catattgctg gccggactcc cagcgaacga
      901 aggggtcggt gtcgtgcgcg gcgaggaact gcaactgcgc cagcggcagc ccggacagct
      961 ttaccggcgc ggaaaaacgc cgcagcagcg atggcaccgg cggctggtcg atgccatcga
     1021 atgcaaagct ctgctctggc tccgacaaca gcagcgtgcg cgaagcgatc tcgtgcccag
     1081 cgtcatcgag caagcccatc acgacgggga tcacgagcgg ctgcttttcc ggctcgccag
     1141 gcgtcggcgc cgtgtgctgc ttcagcgtca gtgtatagcg acgcgcggcg gcatcgtgcg
     1201 cggttgagac cgtcacctga ggcgtgccag cctgatggta ccagcgcctg aagccggtca
     1261 ggtcgacacc ggatgcatcc tgcatcgcct gggcaaagtc attgatggtg acagcgtgat
     1321 tgtcgtgccg ttggaaatac aggtccatgc cgcggcgaaa cgcctcgcgc ccgatgatgg
     1381 tggccatcat gcggatcacc tcggcgcctt tgttgtagac agtcgcggtg tagaagttat
     1441 cgatggtgat gtagtggtcg ggctgcaccg gatgcgccag cggtccggca tcctcgcgga
     1501 actgcgcggc ccgcaacgcg cgcacgtcgc caatacgttt gaccacacgg cttccctgat
     1561 cggccgagaa ctcctggtcg cgatagaccg tcagcccttc cttcagcgaa agctggaacc
     1621 agtcacggca ggtgacccgg ttgccggtcc agttgtggaa atactcgtgg gcgatgacgg
     1681 actcgatacc ctggtaatcg ttgtcagtgg cggtctcagg cttcgccagg acgtatttcg
     1741 tgttgaagac gttcagcccc ttgttctcca tcgcccccat gttgaagtcg gacacggcgg
     1801 cgatgttgaa cacgtcgagg tcgtattcca ggccgaacac gtcctcatcc catttcatcg
     1861 aggtcttcag cgagcgcagg gcatgcgcgc atcgatcctc atcgccgcgg cgcacccaga
     1921 cgccaagctg cacgtcgcgg cctgaacggg tggtgaagct gtccttcacc gcgaccagat
     1981 cgccggcgac cagcgcaaaa aggtagcaag gcttggggtg tggatcggtc cagatgatgc
     2041 gatgccgtcc gccgcccgcg tcctccaccg cacttggatt gccgttcgac agcatcaccg
     2101 gcacgcgtgc cttgtcggcg gtaatgctga cggtgaatcg cgccatcacg tcgggtcgat
     2161 ccggaaaata ggtgatgcgc cggaaacctt ccgcctcgca ttgggtgaag tagctgccgt
     2221 tcgacgtgta gaggccagaa agctccgtgt tgtccttcgg tgcgatctgc gtctcgatct
     2281 ccagcactgc tgtgtcgggc atgtcgggga tcagcagctt gccgtcgtcg atgcgatagc
     2341 gattggcgcc aagttccacg ccgtcgagcg cgatgcgcac caacttcagc gcctcaccgt
     2401 cgagcagcag cgctcccgtg ccctggctct ccggattgcg ccggaccgac agacgcgaac
     2461 gcaccttggt ggctgtcgca tcgaggtcga aggcgagatc ggccgtatcg accaggtagg
     2521 cgggtggacg atagtcggca aggcgggtct cgtgcggtgc ggtctgcgac atcagcgtag
     2581 gtccttcagc aaactcactt tggatatatg tgcgtccaat cgcgcgggaa cggcaattcg
     2641 tcctcggtct tcagcgtcac gtggatcaaa tgccaggccc cgttgtccac ctgatatagc
     2701 aggcgcacga atagaaaatt cttggcgaaa tcgatcttgt agatcacgtc cttcccggaa
     2761 cggccgtaat cgcgcgcata caccagatcg gtgtactggg cagcgccgag gtcctttacg
     2821 aaacggaatg tgccacgcag ccggtcggcg ggcgttgact ccatcagcct ggcggcggct
     2881 tcagccgcgg cgtccaggtc ctgatgcgcg atattgttga cgatcgccga actggccgcc
     2941 agcgcatcga acggcgcagg ctgggcgact gccgcgccgg cgaggcacag cgatgcggca
     3001 atagcaagat gccgcatcgc tgccgtcgcg gttatcgcac cacggcgatc tgttcgcggc
     3061 gcaactgcac cgtgagctcg tcgagcgcct tgccg
//
LOCUS       DATGEM010000526        10926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_31450, whole genome shotgun sequence.
ACCESSION   DATGEM010000526 DATGEM010000000
VERSION     DATGEM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31450"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..283)
                     /locus_tag="VKQ27_16085"
     CDS             complement(8..283)
                     /locus_tag="VKQ27_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLY90502.1"
                     /translation="MNCKRLVISGRVQGVGFRDWIAMKATELGLAGWVRNRIDGSVEA
                     LIAGDIAAVEEMSRLCRRGPRMATVVSIEEDLADPPDHFDFRRYPTG"
     gene            complement(280..999)
                     /locus_tag="VKQ27_16090"
     CDS             complement(280..999)
                     /locus_tag="VKQ27_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HLY90503.1"
                     /translation="MKRFWDRATAEPADGGYTILLDGKPMHLPGGGVLCVTGEPMAEA
                     IAAEWQAAGGTRGGEMTFADTPLTRLAGTAQQRITPDPAATIDAIARYAETDLLCYRA
                     DTPPALVERQTREWQPLLDWAAQRYDAPLRVSSGVAYVKQHRGAIAALRTAVAALDVP
                     ALTALGIAVPALGSLVLGLALAEGRLDAATAHALGALDELFQAEAWGEDPEAAARRAN
                     IAADIALAERFLRLTRPTVPA"
     gene            complement(996..3353)
                     /locus_tag="VKQ27_16095"
     CDS             complement(996..3353)
                     /locus_tag="VKQ27_16095"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLY90504.1"
                     /translation="MASRAVRISIWSIVALAVLVGGGIGVVIATFDPNSYKPQIVAAV
                     KRATGRDIDLKGNIHLALSLQPTLTVQDVSFSNPPGFSRPQMATLQQLDLQLALLPLI
                     HHQIEIDRLVLVKPDILLETNAKGQSNWTFTPQSAPSAPTAPTSGTQSGEATQTNINV
                     EMLEVQNGTVTWRDNTTNRTAVLGVTTLTVAAPSPDANLHLAMNATYNGNAFILAGDV
                     GPLTRLQQSAAKTAWPIQLTVQAAGAKLALDGSIAQPEQARGYTMKINATVPDLTALA
                     PFAGGATLPPLHDIMVAAQIADSGHPLPDVSSLALHVGASDLSATVPGLKLAKLDVAI
                     PKPDQAAQIALQATLDDQPATLNGTAGLLPVLLSGGKTTNPIPMDLAAQLLGSSISVK
                     GTAAYANGLPSLQAAIKSEKLDADALIAALAKPTAPQPAAGAPASAPAPAPAPKPAAS
                     NRIFPDTPLPFAMLRKADANVTLAITDMIWDKAQYKSIVAHLDLHGGALKVDQIAADL
                     PAGHLSGALTAADTPPAPPVTIRLDAPALALGPLLAAMGKPGYVSGNLEVHANLSGAG
                     ASPHAIASSLNGTLGLAMANGTVDNRLLGSTLGSVLNAVNALDLVGRGGTSAVQCFAA
                     RLNANHGIGTLQPLVLSSSLITMEGGGTVNLGTETMDLRLRPQGRVAGAPIVVPLKVE
                     GPIRAPSVNSDTAATIGANAGTVAGTVLGSATPLGLVTGALGGQKLLGGGGEAVNCGA
                     ALAAARGEAAPAAATAPHPAPAQAPTPQQQPKLPNPGQLLQKLFR"
     gene            complement(3369..4358)
                     /locus_tag="VKQ27_16100"
     CDS             complement(3369..4358)
                     /locus_tag="VKQ27_16100"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLY90505.1"
                     /translation="MAVATRTVTQDEADIRLDRWFRRHFPGLTQGAIQKLCRTGQVRV
                     DGHRAEAATRLTAGQSVRIPPLPDATQPQPKPTPRIDPNTARDLQRMVIYQDDHVIAL
                     NKPPGMPVQGGPGITHHLDAMLDALRFGAPDRPRLVHRLDRDTSGVLLLARTAGTAAK
                     LAAAFRTRAVEKAYWAVTARTPVPHEGRIDLPLRRIGGARGERTEVAPDDPDAARAIT
                     DYRTIDHAGQKLAWLELKPLTGRTHQLRVHCVAIGAPILGDVKYARPDQNNAFAATIE
                     GLSSELHLHARALRLPHPAGGTLLVEADLPPHMTQTFRTLGFHAPPARRPERR"
     gene            complement(4542..4841)
                     /locus_tag="VKQ27_16105"
     CDS             complement(4542..4841)
                     /locus_tag="VKQ27_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLY90506.1"
                     /translation="MITCCIRYTLDPHRITDFERYAQTWPPIIARCGGALVGYFLPKE
                     GPNNFALALIDFENLAAYERYREALRADPEAQDNFAYAQRTQCIQIEDRCFLRRG"
     gene            complement(4843..5151)
                     /locus_tag="VKQ27_16110"
     CDS             complement(4843..5151)
                     /locus_tag="VKQ27_16110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HLY90507.1"
                     /translation="MQTTQITGHLAVTALWEARDGEADAVADILARFAPQARQEPGTQ
                     LFQVHRSLENPSHFLFYEVFDDSAAYEAHQQTPHFKALIQGEGLPRLSKRERTQYRPL
                     "
     gene            complement(5407..5640)
                     /locus_tag="VKQ27_16115"
     CDS             complement(5407..5640)
                     /locus_tag="VKQ27_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90508.1"
                     /translation="MESNLATFLTIPLEELAVSFDRHLRVLKNCTAARQRSGSPLTGA
                     AVAHVHQHRVTGGPDTQGSAVTLCDPFHLFLPL"
     gene            complement(6005..7414)
                     /locus_tag="VKQ27_16120"
     CDS             complement(6005..7414)
                     /locus_tag="VKQ27_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HLY90509.1"
                     /translation="MSWTHTFHPTTLREYDIRGTIGRTLSEADAYAIGRCFGSTVVRK
                     GGRTVAVGYDGRLSSPSLERALVEGLGASGIEAVRIGCGPTPMLYYANVQQKTDGAVM
                     VTGSHNPPDYNGFKMVLGGKPFFGKDIQALGSQAAAGDVEGETVTPQRSVDIAPDYVA
                     RLLADWDGGDRLLKAVWDNGNGAAGEVLKKLIPGLPGEHTLLNGDIDGHFPAHHPDPT
                     VPANLQQLIAEVAARKADIGIALDGDADRIGLVDNKGRILFGDQLLVLLARDVLRTHP
                     GATIIADVKASQVLFDEVARAGGNPLMWRTGHSLIKAKMAETGAPLAGEMSGHTFFAD
                     HWYGFDDALYAAVRMLGVLARMDGKLSDARDALPQVINTPEVRFDCDDTRKFAVIEEV
                     AARLRAEGAKVSEIDGVRVQTDEGWWLLRASNTQAVLVARAEATTEAGLERLKGALVK
                     QLEQSGLPAPDFSGSHAGH"
     gene            complement(7416..8294)
                     /gene="galU"
                     /locus_tag="VKQ27_16125"
     CDS             complement(7416..8294)
                     /gene="galU"
                     /locus_tag="VKQ27_16125"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HLY90510.1"
                     /translation="MIKPLRKAVLPVAGLGTRFLPATKATAKEMLPVVDKPLIQYAVE
                     EARAAGIEQFCMVTGRGKTALVEHFDVAFELEATLRERNKTAELEMLRATQTEPGSIV
                     TVRQQVPLGLGHAIWCARAFIGDDPFAILLPDDLVLAETPCLRQLADVYRDTGGNVVA
                     VTEVPREQTNRYGILHIGKDDGRLVEVKGLVEKPAPKDAPSNLSIIGRYVLMPEVITH
                     LARMERGAGGEVQLTDGMARLIGNQPFHGLRYEGKRFDCGDKAGFLEAQIAYALKRPD
                     LAEAVRGFLRHYVQFC"
     gene            complement(8416..8925)
                     /locus_tag="VKQ27_16130"
     CDS             complement(8416..8925)
                     /locus_tag="VKQ27_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HLY90511.1"
                     /translation="MLWLLLGALTLFLFLGGLRAFERASITTIKSLLAWIAALGGVTL
                     ALLLILTGREGIALGALAMFGPLIWQRWKAYRASLNSGANRQQQSSGSAGGDSQRPPP
                     RQPGAGMTPEEAYQVLGLHRGATEAEIRAAHHRLMRGAHPDSGGSDWLATRINLARDV
                     LLRHSKRTG"
     gene            complement(9030..9692)
                     /locus_tag="VKQ27_16135"
     CDS             complement(9030..9692)
                     /locus_tag="VKQ27_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90512.1"
                     /translation="MTDSLPDDARLRATEAQMRRALGLGSEGPTQPAPMAPPPTPAMG
                     LHPQRRRFVRDGEVVVQHKEHSAAGETGTNQLAAARQALREQFAAREEAERALAEVQS
                     ANRDLQTKLAHERLARDETVNRAEAARQAAEQALQAVREELESERARRLQAEERSAVT
                     QERLHESPRQADLLEPAPAPRRRGRPPKVRQTEPLTEPAEGQDQADFVEWWVPGWQDR
                     FR"
     gene            9846..10814
                     /locus_tag="VKQ27_16140"
     CDS             9846..10814
                     /locus_tag="VKQ27_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HLY90513.1"
                     /translation="MGIILGEGEYRYSVVEGWGKLPDGWTFHEVGAVGVDRNDNVYVF
                     NRGEHPMIVFDRLGNFLRSWGEGMFPRAHGVHIAPDDTIWLTDDGAHCVRRCTLDGKV
                     LMTLGTPGKPAPFMSGQPFCRCTHTALSPKGEIYVTDGYGNAQVHKYTPEGKYLMSWG
                     KCGIREGEFNLPHNIHCDADGWVYVADRENNRVQVFDGNGRFETAWNNVVHRPSALCM
                     TGGACPLCFIGEVGPYLGSNRGFPNLGPRVSILSNTGKLLARLGSETNPHGQAPGQFM
                     SPHGIAVDSRGDIYVGEVSVASWPSLFPGEPRPANLRSLQKLMKVA"
BASE COUNT         1753 a         3181 c         4020 g         1972 t
ORIGIN      
        1 gaggtgttca gcccgtcgga tagcgcctga agtcgaagtg atccggggga tcggccaggt
       61 cttcctcgat gctgacgacg gtagccatgc gagggccgcg gcggcaaagg cgcgacattt
      121 cttccaccgc agcgatgtcg ccggcgataa gggcttcgac cgagccgtcg atgcggttgc
      181 ggacccagcc agccaggccg agttcggtgg ccttcatcgc gatccaatcg cggaagccga
      241 cgccctgcac gcggccgctg atcacgaggc gcttgcagtt catgcgggca ccgtcgggcg
      301 cgtgaggcgg aggaagcgct cagccagtgc gatgtcggcg gcgatgttgg cgcggcgggc
      361 ggcggcttcg ggatcctcgc cccaggcttc ggcctgaaac agctcgtcga gcgcgcccag
      421 tgcgtgggcg gtcgcggcgt ccagcctgcc ctcggccagt gcgaggccga gtacgaggct
      481 gccgagcgcg ggcacggcga tgccgagggc ggtcagcgca ggcacgtcga gggcggcgac
      541 ggcggtgcgc agggcggcga tcgcaccgcg gtgctgcttc acgtaggcca cgccggaact
      601 tacgcggagc ggcgcgtcgt agcgctgcgc ggcccagtcg agcaaaggct gccattcgcg
      661 ggtctggcgc tccaccaggg caggaggtgt gtcggcgcgg tagcacagca ggtcggtctc
      721 ggcgtagcgg gcgatcgcgt caatggtggc ggcggggtca ggcgtgatgc gctgctgggc
      781 ggtgccggcg agccgggtca agggcgtgtc ggcgaaagtc atctcgcccc ccctggtgcc
      841 gccggcggcc tgccattctg cggcgatcgc ctcagccatg ggttcgccag tgacacacag
      901 gacgccgccg ccagggagat gcatcggctt gccgtcaagc aggatggtgt agccgccgtc
      961 cgccggctcc gcggtggctc ggtcccagaa tcgcttcatc ggaacagctt ctgaaggagc
     1021 tgccctgggt tcgggagctt gggttgctgc tgaggcgtgg gtgcttgggc cggcgctggg
     1081 tggggcgcgg ttgcggcggc gggggcggcc tcgccgcggg cagcggccag cgcggcaccg
     1141 cagttcaccg cctcgccacc gccgcctagc aatttttggc cgccgagggc accggttacg
     1201 aggccgagcg gtgttgcgct gccgagcacg gttccagcta ccgtgccggc attggcaccg
     1261 atggtggcgg cggtgtcgga gttcaccgac ggcgcacgga ttgggccttc gaccttcaag
     1321 gggacaacga tgggggcgcc ggcgacgcgg ccttgggggc ggaggcggag gtccatggtt
     1381 tccgttccga ggttgacggt gccgccacct tccatggtga tcagcgatga actgagcacc
     1441 agcggctgga gggtaccgat accgtggttc gcgttgagcc gggcggcgaa gcactggaca
     1501 gcactggtgc caccgcgccc gaccaggtcc agcgcgttga ctgcgttgag gacggaaccg
     1561 agcgtactgc cgagcaggcg gttgtccact gtgccgttgg ccatggcgag gccgagcgtc
     1621 ccattgaggc tggatgcgat ggcgtggggc gaggcgccgg cgccgctcag gttggcgtgc
     1681 acctcaagat tgccggagac gtagccgggc ttgcccatgg cggcgagcaa ggggccgagg
     1741 gcgagtgcgg gcgcgtcgag acgtatcgtc accggaggtg cgggcggcgt gtccgccgct
     1801 gtcagggcgc cggacagatg gccggctggg aggtcggccg cgatctggtc aaccttcagt
     1861 gcgccaccgt gcaggtcgag atgggcgacg atcgacttgt actgggcctt gtcccagatc
     1921 atatcggtaa tcgccagggt cacattggca tcggccttgc gcagcatggc gaagggaagt
     1981 ggggtgtcgg ggaagatccg attgctcgcc gctggcttgg gggcaggagc gggtgcaggt
     2041 gccgaggcgg gggcaccggc ggcgggttgg ggtgccgttg gcttggcgag cgcggcgatc
     2101 agggcatcgg catcgagctt ctcggacttg atggcggcct ggagcgaggg aagtccgttc
     2161 gcataggcgg ccgtgccctt cacgctgatg ctggagccga gcagctgcgc agccaggtcc
     2221 atcgggatgg gattggttgt cttgccaccg gagagcaaga ccgggagcag gccagcagtg
     2281 ccattcagcg tcgcgggctg gtcgtcgagg gtggcctgca gagcgatctg cgcggcctgg
     2341 tccggcttcg ggatcgcgac gtcgagcttg gcgagcttca acccgggaac ggtcgcgctg
     2401 agatccgagg cgccgacatg cagcgcgagg ctcgagacat cgggaagcgg gtgaccgctg
     2461 tcggcgatct gggcggcgac cattatgtcg tgcagcggag gaagggtggc gccgccggcg
     2521 aagggggcga gcgccgtcag gtcaggcact gtggcattga ttttcatggt gtacccgcgg
     2581 gcctgctcgg gctgggcgat gctgccgtcc agcgcgagtt tggcaccggc cgcctggacg
     2641 gtgagctgga tgggccaggc ggtcttggca gcggactgct gaaggcgggt cagtgggccg
     2701 acatcgccgg cgaggatgaa cgcattgccg ttgtaggtgg cattcatcgc caggtgcagg
     2761 ttggcgtctg gcgaaggcgc agcgacggtg agagtggtga cgccaaggac agcggttcgg
     2821 ttcgtcgtgt tgtcacgcca ggtgacggtg ccgttctgga cctcaagcat ctcgacattg
     2881 atgttggtct gggtcgcctc gccggactgg gtccccgagg tgggagcggt gggcgccgac
     2941 ggcgcagact gcggcgtgaa ggtccagttc gattggccct tggcgttggt ctccagcagg
     3001 atgtcgggct tcaccaggac aaggcgatcg atttcgatct ggtggtggat cagggggagc
     3061 agggcaagct ggaggtccag ctgctggagc gttgccatct gcgggcggga gaagcccgga
     3121 ggattgctga agctgacgtc ctggacggtg agcgtgggct gcagcgagag ggcgaggtgg
     3181 atattgccct tcaggtcgat gtcgcggccg gtggctcgtt tcacggcggc gacgatctga
     3241 ggcttgtagc tgttggggtc gaaggtggcg atgacgacac cgatgccgcc gccgacgagc
     3301 accgccaatg cgacaatcga ccagatgctt atgcggactg cgcggctcgc catgcggtgc
     3361 aataccccct aacgacgttc tggccggcgc gccggcggcg cgtggaaacc gagcgtgcga
     3421 aatgtttgtg tcatgtgggg cggcaggtcc gcctccacca gcaacgttcc gcctgctgga
     3481 tggggaagcc gaagcgcacg cgcatgcaag tgaagttcgc ttgagagacc ctcgatagtg
     3541 gcggcgaagg cattgttctg gtcggggcgg gcgtatttga catcgcccag gatgggcgcg
     3601 ccgatggcga cgcagtggac gcggagttgg tgagtgcgac cggtgagggg cttcagttcc
     3661 agccaggcga gtttctggcc agcgtgatca atggtccggt aatcggtgat ggcccgggcg
     3721 gcatcgggat catcaggcgc cacttccgta cgttcgcctc gggcgccccc gatgcggcga
     3781 agtggaagat caatgcggcc ttcgtgcggg acgggcgtcc gggcggtgac ggcccagtag
     3841 gctttttcga ccgcacgggt acggaatgcg gccgcgagtt tggctgctgt gccggcagta
     3901 cgggcgagga gcaggacgcc ggatgtgtcg cggtccaggc ggtgcaccag gcgggggcgg
     3961 tcaggggcgc cgaaacggag tgcatcgagc atcgcgtcga ggtggtgggt gatgccgggc
     4021 ccgccttgca ccggcatgcc agggggcttg ttgagcgcga tgacgtggtc gtcctggtag
     4081 atgaccatgc gctggagatc gcgggcggtg ttggggtcga ttcttggagt gggtttgggc
     4141 tgaggctggg tcgcgtccgg cagtgggggt atgcggactg actggccggc agtaaggcgg
     4201 gtggcggcct cggcgcggtg gccatccacg cggacctggc cggtgcggca cagcttctgg
     4261 atcgcaccct gcgttaagcc agggaagtgg cgacggaacc accggtcgag gcggatgtct
     4321 gcttcgtcct gggtaacggt gcgagtggcg acggccatgc gtggggttta tcgcgggacg
     4381 tcgcattggg ggagggggct cgtccgctga tctgtcgaca gcatacgcat cgcgttcgat
     4441 cgtcgctgtc gccaaccgct cgccggttcc cgtcatgccg aacgaagcat gccgccggcg
     4501 aaggccgatg ggcttggcat aacgggcgcc gggaaccggc cttaccctct tcgcaggaag
     4561 cagcggtcct cgatctggat gcactgcgtg cgctgggcgt aggcgaaatt gtcctgcgct
     4621 tcggggtcgg cacggagggc ctcgcggtag cgttcgtagg cggcgaggtt ttcgaagtcg
     4681 atcagcgcga gggcgaagtt gttgggccct tccttgggga ggaagtagcc cactagcgcg
     4741 ccaccgcagc gcgcgatgat cggcggccag gtctgcgcgt accgctcgaa gtctgtgatg
     4801 cgatgaggat cgagcgtgta gcggatgcaa caggtgatca tatcacagcg gccggtactg
     4861 agtgcgctcg cgtttgctga gacgcgggag gccttcgccc tggatcagcg ccttgaagtg
     4921 cggggtctgc tggtgagcct cgtaggccgc ggagtcgtcg aacacttcat agaagaggaa
     4981 gtgagaggga ttctccagcg agcggtggac ctggaagagt tgcgtgccgg gctcctgccg
     5041 ggcctggggc gcgaagcgtg cgaggatgtc agcgacggcg tcggcctccc cgtcgcgggc
     5101 ttcccaaagg gccgtgacgg cgagatggcc ggtgatctga gtggtctgca ttgtggtcct
     5161 cctgctgttg ggaacaggga ggacgtaggg gaattgcgcg tgcggatcag cagtgaaggc
     5221 ttcggtgtag ggcgaagggt ttgggggacg gaggccctta ctggtagtgc ctcggtctga
     5281 attttggttt aggctgatga gcgaccgccc ggtttcagat ggcggcggag gggagcgcga
     5341 agcggccctc ctcattccgt gttcgccagg ccgttcggac gtgaacgatg ccggcgacac
     5401 tcgcactcaa agaggcagga agagatggaa aggatcgcac aatgtcactg cggatccctg
     5461 cgtgtcagga cctccggtga cccggtgctg gtgaacatgt gccactgccg cgcctgtcaa
     5521 cggcgaaccg gagcgttggc gagcagcggt gcagtttttg agaacgcgca ggtgacgatc
     5581 gaaggaaaca gcaagctctt cgagcgggat ggtcaggaag gtcgcaaggt tcgattccac
     5641 ttctgccctg aatgcgggac atctttgtat tggcatcccg atatacgtcc agagtggtgc
     5701 atcgtcgcgg tcggggggtt cgcggacccg gactttccgc cgccgtcggc gtccattttc
     5761 gaggagacac ggcataagtg gaccatgctc ccggagtcga tgaagcacgc gcagttatcg
     5821 ttgcggcatt aggtgcgatg cgaaggatgt ggacacaatg tccggcaatt cagcgcctgt
     5881 ggcgtgcgag gtgccggttc attgcgcgag atacgaaaac gacgaagcgg cactggtcgt
     5941 agtgcacacc ttagccggga ttgcttcgtc gctgcgctcc ttgcaatgac agggtgggcg
     6001 ccgttcagtg ccccgcgtgg ctgccggaaa aatccggtgc cggcaggccg gactgttcca
     6061 gttgcttcac cagcgcgccc ttcagccgct ccagccctgc ctcggtagtg gcctcggcgc
     6121 gcgcaaccag cactgcctgc gtattggaag cgcggagcag ccaccagccc tcgtcggtct
     6181 gcacgcgaac gccgtcgatc tccgacacct tcgcaccctc ggcacgaaga cgcgccgcga
     6241 cttcctcgat caccgcgaac ttgcgcgtgt cgtcgcagtc gaagcggacc tcgggcgtat
     6301 tgatcacctg cggcaaggca tcgcgcgcgt cggacagctt gccatccatg cgcgccagca
     6361 cgcccagcat acgcaccgcg gcgtagagcg cgtcatcgaa gccataccaa tgatcggcga
     6421 agaacgtgtg ccctgacatc tcgccggcca gcggcgcgcc cgtctcggcc atcttcgcct
     6481 tgatcagcga atggcccgtg cgccacatca gagggttgcc gccggcgcgg gcgacctcgt
     6541 cgaacagcac ctggctggcc ttcacgtcgg ctatgatcgt tgcgccggga tgtgtgcgca
     6601 gcacatcgcg ggcgagcagc accagaagtt gatcgccaaa caggatgcgg cccttgttgt
     6661 ccaccaggcc gatgcggtcg gcgtcgccat ccagcgcaat gcctatatcg gccttgcggg
     6721 ccgcgacctc ggcgatgagc tgttgcaggt tcgcgggaac cgtcgggtcg ggatggtgcg
     6781 cggggaagtg gccgtcgatg tcaccgttca gcaatgtgtg ctcgccgggg aggccgggga
     6841 tcagcttctt cagcacctcg cccgcggcgc cgttgccgtt gtcccacacg gccttcagca
     6901 aacggtcacc gccatcccag tcggcgagca ggcgcgcgac gtaatcgggc gcgatatcga
     6961 cgctgcgctg cggagtgacg gtctcgccct cgacgtcgcc ggctgcggcc tggctgccga
     7021 gcgcctggat gtccttgccg aagaacggct tgccaccgag caccatcttg aagccgttgt
     7081 agtccggcgg attgtggctg ccggtgacca tgactgcgcc atcggtcttt tgctgcacgt
     7141 tggcgtaata cagcatcggc gtggggccgc agccgatgcg aaccgcttca atgccgctgg
     7201 cgccgaggcc ctcgacgagt gcgcgctcca gcgaggggct ggagaggcgg ccatcgtacc
     7261 cgacggccac cgtacggccg cccttgcgga cgacggtgga gccgaagcag cgaccgatcg
     7321 cgtaggcatc ggcttcggaa agggtgcggc cgatcgtgcc acggatgtcg tattcgcgca
     7381 gcgtggtcgg gtggaaggtg tgggtccagc tcatctcagc agaattgcac gtagtggcgg
     7441 aggaaaccac gcacggcctc agcaagatcg ggacgcttca gggcataggc gatctgggct
     7501 tccaggaagc ctgccttatc gccgcagtcg aagcgcttgc cttcgtagcg caggccgtgg
     7561 aagggctggt tgccgatgag cctggccatg ccgtcagtga gctgcacttc gccgccggcg
     7621 ccgcgttcca tacgcgcgag gtgggtgatg acctcgggca tcagcacgta gcggccgatg
     7681 atggagagat tggagggcgc gtccttgggg gcgggcttct ccaccagtcc tttcacttcc
     7741 acgaggcggc catcgtcctt gccgatgtgc aggataccgt accggttggt ctgctcgcgc
     7801 gggacttcgg tcacggcgac gacgttgccg ccggtgtcgc gatagacgtc ggcgagctga
     7861 cgcaggcagg gggtttcggc gagcaccaga tcgtcgggaa gaaggatcgc gaagggatcg
     7921 tcaccaatga aagcacgggc gcaccagatg gcgtggccga ggccgagtgg cacctgctgg
     7981 cggacagtaa cgatcgagcc gggctcggtc tgggtggcgc ggagcatttc cagctcggcg
     8041 gtcttgtttc tctcgcgcaa ggtggcttcg agttcgaatg cgacatcaaa atgttccacc
     8101 agcgcggtct tgccgcggcc ggtgaccatg cagaactgct cgatgccggc agcacgcgct
     8161 tcctccaccg cgtactggat cagcggcttg tccaccaccg gcagcatctc cttggcggtg
     8221 gctttggtag cgggaaggaa gcgggtgcca agaccggcga ccggcagcac ggctttgcgt
     8281 aatggcttga tcacaattgg gatccccctg tttcccgaag gccagcagca cggctggcag
     8341 gcggaaatgt ggcgtcaaca acgaacccgg tggcgacacg tcagtgcgag acgtatcacg
     8401 atggcgcgcg ggacgtcatc cggtacgttt ggagtgccgc agcagaacat cgcgcgccag
     8461 gttgatgcgg gtggcaagcc agtccgagcc gccgctgtca gggtgggcgc cccgcatcag
     8521 gcggtggtgg gcggcgcgga tttccgcctc ggtggcgccg cgatgaaggc cgagcacctg
     8581 ataagcttct tcgggcgtca taccggcgcc gggctggcgg ggcggtggac gctggctatc
     8641 gccgccggcc gagccgctgg attgctgctg gcgattcgcg ccgctgttga ggctggcgcg
     8701 ataggccttc cagcgttgcc agatcagcgg gccgaacatc gcaagggcgc cgagcgcgat
     8761 tccctcacgg ccggtgagga tcagcaacag ggcaagtgtg acgccgccga gggcggcgat
     8821 ccaggcgagc agcgacttaa tggtagtgat agacgcgcgt tcgaacgcgc gaaggccgcc
     8881 gaggaaaagg aacagcgtga gggcgccgag gagcagccag agcatggtgc tcaacgtagg
     8941 cgatgtatgg tggcagtcct actggtggtt ttcactgaac ccggaacgga ttcggggcct
     9001 catccagagg ctgatgccag gctggccgcc tagcggaacc gatcctgcca gccggggacc
     9061 caccactcga caaagtccgc ctgatcctga ccttccgcgg gctccgtcag cggctcggtc
     9121 tggcggacct tcggtgggcg gccacgccgc cgcggggcag gagcgggttc cagcaggtct
     9181 gcctggcggg gcgattcgtg gaggcgttcc tgcgttacgg ctgatctttc ctctgcctgg
     9241 aggcgacggg cgcgctcgga ctccagttcc tcgcggacgg cctgcaatgc ctgttcggca
     9301 gcttggcggg cggcttccgc gcgattgacc gtttcgtccc tggcgaggcg ttcgtgtgca
     9361 agcttggtct gcaggtcacg gttggcagac tgcacctcag ccagagcccg ctccgcttcc
     9421 tcgcgcgccg cgaactgctc gcgcagtgcc tgacgtgcgg cggccagttg gttggtgccg
     9481 gtctcaccag cggcggaatg ctccttgtgc tgaacgacca cttcaccgtc gcgcacgaag
     9541 cggcggcgct gtggatgcaa tcccatggca ggtgttggcg gaggggccat cggtgccggc
     9601 tgggtcgggc cttcgctgcc caggccaagg gcacggcgca tctgcgcctc ggtcgcacgc
     9661 aaccgtgcgt cgtctggcaa agagtcggtc atggttttct tttaggtcac ccgaatttcg
     9721 ttcactcaat tctataatgg ccggtgggat tcggccagcg cgcgcgtgct gtacctgtcg
     9781 tcggcccgta tgattgattg atacagcggt tggtgcatgg ataccagacg atcggagggg
     9841 acgacatggg cattattttg ggcgaaggcg aatacagata tagtgtggtg gaaggttggg
     9901 gcaagcttcc ggacggctgg acttttcatg aggttggtgc tgtcggcgtc gatcgcaacg
     9961 acaatgtcta tgttttcaac cgtggcgagc atccgatgat cgtgttcgat cgcctgggaa
    10021 atttcctgcg atcatggggc gaaggcatgt tcccgcgcgc tcacggcgtg catatagccc
    10081 ccgacgatac aatctggctg accgacgacg gcgcccactg cgtccggcgc tgtacgctgg
    10141 acggcaaggt cctcatgacg ctcggcacgc ccggcaaacc tgcgccgttc atgagtggcc
    10201 agccattttg ccgctgcacg catacggcgc tttcgccaaa gggtgaaatt tatgtcaccg
    10261 atgggtacgg taacgcgcaa gtacacaagt atacacccga aggaaagtat ttgatgtcgt
    10321 gggggaagtg tggcatccga gagggagagt tcaacctgcc gcacaatatc cactgcgacg
    10381 cggacgggtg ggtttacgtg gccgatcgcg agaacaatcg cgtgcaggtg ttcgacggca
    10441 acgggcgctt cgagaccgcg tggaacaatg tcgtgcaccg gccgagtgcg ctgtgcatga
    10501 cgggcggtgc ctgtccgttg tgcttcatcg gcgaggtcgg tccgtatctg ggatccaatc
    10561 gtggtttccc caatctgggg ccgcgggtca gcattctgtc caataccggg aagctgctgg
    10621 cgcgacttgg gtctgagacc aatccgcatg gccaggcgcc agggcagttc atgtcgccgc
    10681 atggaatcgc agtggattcg cgtggcgata tttacgttgg cgaggtttcg gttgcgtcgt
    10741 ggccttcgct gttcccgggc gagcctcggc cggccaatct ccgcagcctg cagaagttaa
    10801 tgaaggtcgc atagccactg cgtcgggcct gccgccgcgg tattactgta gatgcatcgc
    10861 ggcggcagct ttcgccggtc caacctccgg tcacgccccg tcattggatt caccgcagcg
    10921 atccag
//
LOCUS       DATGEM010000527         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1081281, whole genome shotgun sequence.
ACCESSION   DATGEM010000527 DATGEM010000000
VERSION     DATGEM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081281"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1379)
                     /locus_tag="VKQ27_16145"
     CDS             complement(138..1379)
                     /locus_tag="VKQ27_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLY90514.1"
                     /translation="MIRTIGIDFGTTNSVVALLHDDGSVTTRRHASLDVFRSVLCFWA
                     EPGAGRVHLNHAAGPEAITAYLEDPLDSRLIMSMKTYLAQKSFTETRIFAHPFTLERL
                     IATFLRALLDGVPTAPRVVVGRPVRFAGEFVDDMLGAARLSGAYAEAGFPDVVQALEP
                     EAAGARFARTLAEPATVLIGDFGGGTSDFSVLRFEPRAGTRPLAHSGIGIAGDVFDYR
                     IIDRAIAPLLGKGDTYRVMGKPLPVPPEYYSGFARWHLLSLMRNPRTLRAISEVARTA
                     QHPDRLRSLMTLIEDGLGYALYQAVSTAKAELSRSEATTLQFAHKSFVVERDVTRDEF
                     ESWIASDLARLAATVDKVLAEAALRPEAIDRVFLTGGTAFVPSVRRLFESRFGSEKLE
                     GGGEFVSVAEGLALLATDPPS"
     gene            complement(1478..2602)
                     /gene="ispG"
                     /locus_tag="VKQ27_16150"
                     /gene_synonym="gcpE"
     CDS             complement(1478..2602)
                     /gene="ispG"
                     /locus_tag="VKQ27_16150"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HLY90515.1"
                     /translation="MSYRPYQEIIRRKSRRVYVGRVPVGDGAPISVQTMTNTLTTDVA
                     GTIAQIRRAELAGVDIVRVSCPDQPSALALKEIVHEVNVPIVADIHFHYKRAIEAADS
                     GAACLRINPGNIGSDERVKEVIKAARDHGCSMRIGVNAGSLEKHLLEKYGEPNPEALV
                     ESALYHANILQQHDFHDFKISVKASDVFLAVAAYQQLAEACDHPLHIGITEAGGRRMG
                     TVKSAVGLGSLLWAGIGDTLRVSLSDEPEEEVRVGWDLLKALGIRHRGVKVISCPSCA
                     RQGFNVIQTVQTLEDRLAHIETPITLSIIGCVVNGPGEALMTDIGLTGGGNGRHMVYS
                     AGKTDHTIDGRDMVDHIVELVEAKAAELRAAKEAEKLAAE"
     gene            complement(2673..3671)
                     /locus_tag="VKQ27_16155"
     CDS             complement(2673..3671)
                     /locus_tag="VKQ27_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HLY90516.1"
                     /translation="MMSSTLEQELDAPRAPRAGADLRAARERVGWSLSQMADALRIRY
                     QYLEALEEGRINELPGNAYVLGFIRTYATALGLDPTEIARRFKAEAAEVTEQTVLTFP
                     VPVPERGVPAGAVVLLGVILAVGAYVGWYRLSGEGRLPAETSAPIPARLAPLAQQAIP
                     PSLPPSSTMVAQAPAAQPSEPATQSVQQQAPGAPDQASTSIAMAAPPVAAISPTQAAA
                     APAPAAFTPPSADQTRIVLRASADAWVQVRDRSGTILLNRILHAGDAWEVPPKPNLLL
                     TTGNAGGTDLMVDGSLTPSLGGNGAVRRDVPLDPDLLKDGKVAASSPAMAAKSSTQ"
     gene            complement(3821..6022)
                     /gene="ptsP"
                     /locus_tag="VKQ27_16160"
     CDS             complement(3821..6022)
                     /gene="ptsP"
                     /locus_tag="VKQ27_16160"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLY90517.1"
                     /translation="MAHGTAPLQELAQLVASELVSEVCSIYAARPGEILELVATQGLN
                     PAAVGRTRLRVGEGIIGLCAATAQVMNLPDAQNHPAFAYRPETGEEPFASMLAVPVRR
                     AGRTLGVVAVQNRNPRGYSDEEVDELETVAMLLAEVLGTTGAADGAEEGVGATVPRVF
                     AGAPLVPGIAIGQVVLLGRRWRPLHLLADDPDAELARLNDAVQRMQQGLDELIRGAPD
                     GFVSGADTAESREVLDAYRLIAADAGWMKRVSDVIESGLSAEAAVQRVTGELHDRMRR
                     ITDPYLRERLSDLEDLAARLLAVLAGDGPRPTVPPGAILLARRLGPAELLDWHAAGIG
                     GIAVEEASPVGHAAILARALGLPAVGGVRGMLDATGPGDEAVVDADEGQLLLRPDLEV
                     RSSYVRVLEARTAQQAGWATLRQRPAITTDGVHVSLMLNIGLHMEVAQLDATGAEGIG
                     LYRTEIGMLARGAIADVAEQAATYARVLDAAAGRPVVFRTLDLGADKLLPGDAPEEEN
                     PAMGWRSLRIGLDRPALLRRQLRALLLAAGGRPLTVMFPMVATVAEFRAARALLMAEA
                     RRVRPAPERLSIGTMLEVPALMWQLDELLQEVDFISVGSNDLLQFLFAADRSAPALYS
                     RYDLLSQPVLSLLDELLTAARGAKGGAGVPVSLCGEAASRTLEAMTLVGLGFSNLSMP
                     ATGILPVKALLAELDLNAFKPVLTAIRRSASGAASLREPINVWAREHGLPV"
     gene            complement(6071..7117)
                     /locus_tag="VKQ27_16165"
     CDS             complement(6071..7117)
                     /locus_tag="VKQ27_16165"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLY90518.1"
                     /translation="MPPDIAIPGATVAAEAELNRLWSRGTEFLGCRHAIMGGAMSWVS
                     ERHLVAAISNAGGFGVIACGSMEPDMLAAEIAATQALTERPFGVNLITMHPRLDDLIR
                     VCLEARVGHIVLAGGIPPGGAVRAVKAGGARLIAFTPALVLAKRLVRSGADALVIEGS
                     EAGGHIGPVSLIVLAQEILPHIRDVPVFVAGGLGRGEAILTLLEMGASGAQLGTRFAA
                     ASESIAHSNFKRAFIRAAARDAVPSIQLDERFPVIPVRGLVNEGVKRFLRFQAETRDR
                     FEAGQLTKEAAQLAIEHFWAGALRRAVIDGDVENGSVMAGQSVGMVTAEQPTAEIIQE
                     LVGQAVAALAARPR"
BASE COUNT         1203 a         2449 c         2390 g         1169 t
ORIGIN      
        1 tgtccctttg ctgcaccgca accttagagc gggtgggtca ggatgcaaac gccggatctc
       61 ccctcatcct ccaggtttac gcttcgcatt tacaattgta tatccagcga gggcgcatcc
      121 cggaactgct gcggagccta actcggtggg tctgttgcga gcagcgccag gccctcggcg
      181 accgagacga attcgccgcc gccttcaagc ttctcgctgc cgaaacggga ttcgaacagg
      241 cggcgcaccg aggggacgaa ggcggtgccg ccggtcagga agacgcgatc gatcgcctcg
      301 gggcgcagcg ccgcttccgc caggacctta tccaccgtgg cggcaagtcg ggccagatcc
      361 gaagcaatcc atgactcgaa ctcgtcacgc gtgacgtcgc gctcgacgac gaatgacttg
      421 tgggcgaatt gcagcgtggt tgcttcgctg cgcgacagct ctgccttcgc ggtggagacg
      481 gcctggtaga gtgcatagcc gaggccgtcc tcgatgagcg tcatcaggct gcgcagccga
      541 tcaggatgct gcgcggtgcg ggcgacctcg gaaatggcac gcaaggtgcg cgggttgcgc
      601 atcagtgaca gcaggtgcca gcgggcgaac ccgctatagt actccggagg cacgggcagt
      661 ggcttgccca tcacgcgata ggtgtcgccc ttgccaagca gaggtgcgat ggcgcggtcg
      721 atgatgcggt aatcgaacac gtcgccggca atgccgatac ccgaatgcgc aagtggcctg
      781 gtgccagcgc gcggttcgaa gcgcaggacc gaaaagtcgc tggtgccgcc gccgaaatca
      841 ccgatcagca cagtggctgg ctcagccagg gtgcgagcga agcgggcgcc ggcggcctcc
      901 ggttccagtg cctggacgac gtcggggaag ccagcctcgg cataggcgcc gctgaggcgc
      961 gcggcgccca gcatgtcatc gacgaactct ccggcgaaac ggaccggccg gcccaccacg
     1021 acgcgcgggg cagttggtac gccatccagc agagcgcgca ggaaggtcgc gatcagacgt
     1081 tccagcgtga agggatgggc gaagatgcgc gtctcggtga agcttttctg cgccaggtag
     1141 gtcttcatcg acatgatcag ccggctgtcg agcggatcct ccaggtaggc agtgatcgcc
     1201 tcagggcctg ctgcgtgatt gagatggaca cggccggcgc cgggctcggc ccagaagcac
     1261 agcacggaac ggaacacgtc gagcgacgcg tgccggcgcg tggtcacgct gccgtcgtcg
     1321 tgcagcagcg cgaccacgct gttggtggtg ccgaagtcga tgccgatggt gcggatcatg
     1381 gggccgcgcg ttcaagcgca cgcagcctcg gagtgcaaga ggtaacgggg gtcacggatt
     1441 gttgtcgccg gcacgagttg ccccgcgcca gcttgaatta ctccgctgcc agcttctccg
     1501 cttccttcgc agcccgcagc tccgccgcct tcgcttccac cagctccacg atgtggtcca
     1561 ccatgtcgcg gccgtcgatc gtgtggtcgg tcttgccggc gctgtagacc atgtgccggc
     1621 cgttgccgcc gcccgtcaga ccgatatcgg tcatcagcgc ctcgccggga ccattcacca
     1681 cgcagccgat gatggagagc gtgatcggcg tctcgatatg tgccagccgg tcctccagcg
     1741 tctgcaccgt ctggatcacg ttgaacccct ggcgggcgca ggacgggcag gagatgacct
     1801 tcaccccccg atgccggatg cccagtgcct tcagcaggtc ccagccgacg cggacttcct
     1861 cctcgggctc atcggacagc gagacccgca acgtgtcgcc gataccggcc cagagcagcg
     1921 agccaagccc cacggctgac ttcacggtgc ccatacgccg gccgccggcc tcggtgatgc
     1981 caatgtgcag cgggtggtcg caggcctcgg cgagctgctg gtaggcggcc accgccagaa
     2041 acacgtccga cgccttcacg ctgatcttga agtcgtggaa gtcgtgctgc tgaaggatgt
     2101 tggcgtgata gagcgcgctc tccaccaggg cttccgggtt tggttcgccg tatttctcca
     2161 gcaggtgttt ctccagcgag ccggcgttca ccccgatccg catggagcag ccgtgatcgc
     2221 gggcggcctt gatgacctcc ttcacccgtt cgtcgctgcc gatgttgcct gggttgatgc
     2281 gcaggcaggc ggcgccgctg tcggctgcct cgatcgcccg cttgtagtgg aagtggatgt
     2341 ccgcgacgat cggcacgttc acctcgtgca cgatctcctt cagcgccagc gcgctgggct
     2401 ggtccgggca ggagacgcgg acgatgtcca cccctgccag ctccgcccgc ctgatctggg
     2461 cgatcgtgcc ggcgacgtcg gtggtcagcg tgttggtcat ggtctggacg ctgatcgggg
     2521 cgccgtcgcc caccggcacc cggccgacat acacgcgacg cgacttccgc cggatgatct
     2581 cctggtacgg ccgatagctc attgggttgg ctctccgtgt ggtgcggtgc gaaatgcctg
     2641 ccacctacaa tgggggctcc ggcgccaaac cgctactggg tgctggattt cgctgccatg
     2701 gcgggcgaac tggccgccac cttgccatcc ttcagcaggt cggggtcaag cggcacgtcg
     2761 cggcgcaccg ctccgttgcc gccgagggaa ggcgtcaacg acccgtccac catcaggtcc
     2821 gttccgcccg cattgcctgt ggtcagcagc aggttgggtt tcggcggcac ctcccaggcg
     2881 tcacccgcat gcaggatgcg gttgagcagg atggtgccgc ttcgatcgcg cacctgcacc
     2941 caggcgtcgg cgctcgcacg cagcacgatg cgagtctggt cggccgatgg cggggtgaag
     3001 gccgcgggag ccggggccgc ggcggcctgt gtcggcgaaa tcgccgccac cggaggcgcc
     3061 gccatggcga tgctggtcga ggcttgatcc ggcgcgcccg gggcctgctg ttgcacagac
     3121 tgagttgcgg gctcggacgg ctgcgcggcc ggggcctgtg ccaccattgt ggacgatggc
     3181 ggcaggctcg gtgggatggc ctgctgcgcc agcggcgcga gccgtgccgg gatgggcgcg
     3241 ctggtttccg ccggcagccg cccctcgcct gacagccggt accaaccgac ataagcgcca
     3301 acagcaagga tcacgcccaa cagcaccact gcgccggccg gcacgccgcg ctcaggcacc
     3361 gggaccggaa aggtcaacac cgtctgctca gttacctcgg cggcttccgc cttgaagcgg
     3421 cgcgcgattt cggtggggtc cagtcccaac gccgtcgcgt aggtgcgtat gaaacccagc
     3481 acgtaggcgt tgccgggcag ttcgttgatc cgtccttcct cgagcgcttc gagatactgg
     3541 taccgaatgc gcaatgcgtc ggccatctgc gagagcgacc agcccacccg ctcgcgagct
     3601 gcacgcaaat ccgcaccggc cctcggcgca cgcggagcat cgagttcctg ttcgagcgta
     3661 gacgacatca tgcttgggct ttttgccagc gttgatctgc gttgatgcag cacggtcccc
     3721 attcccgcga gcgtgtcact tgcaaacgca cctacactga ctcggtttgc ctggacatgc
     3781 gagtcgtcat ttcggcggtg cgagaaataa aatcgttccg tcaaacaggt aggccatgtt
     3841 cgcgtgccca tacattgatt ggctcgcgca ggcttgcggc gcccgatgca ctgcggcgga
     3901 tcgccgtcag caccggcttg aacgcgttaa gatcaagctc tgccagaagg gcttttactg
     3961 gcaggatgcc agttgccggc atggacagat tgctgaagcc caaccccacg agagtcattg
     4021 cttccagcgt acgcgatgcc gcttcgccac acagggaaac cggcacgccg gcgccacctt
     4081 tcgcgccgcg tgctgccgtt aataattcgt caagcaggct gagtaccggc tgcgacaaca
     4141 gatcatagcg gctgtacagc gcgggtgcgc tgcggtcggc ggcaaacagg aactgcagca
     4201 gatcattcga accgaccgat atgaagtcga cctcctgcaa caattcatcg agctgccaca
     4261 taagtgccgg cacctcgagc atcgtgccga tggatagccg ttcaggtgcc ggtcgcacgc
     4321 gccgtgcctc cgccatcagg agagcgcgcg cggcccggaa ttcggccacc gtggcaacca
     4381 ttggaaacat cactgtcaat ggccggccgc cagcagcgag caacagggcg cgcaactggc
     4441 gccgcagcag ggcgggccga tcgaggccga tacgcagcga gcgccagccc atcgccgggt
     4501 tctcttcctc cggtgcgtcg cccggcagca gcttgtcggc gccaaggtcg agggttcgga
     4561 acaccaccgg ccgtccggcc gcggcgtcca gcacgcgcgc ataggtcgcg gcctgctccg
     4621 ccacgtcggc gatggccccg cgggcgagca tgccaatttc ggtgcggtac aggccgattc
     4681 cttcagcgcc ggtggcgtca agctgagcaa cctccatgtg cagcccgatg ttgagcatca
     4741 gactgacatg caccccgtcc gtcgtgatcg ccgggcgctg gcgcagcgtc gcccatccgg
     4801 cctgctgcgc ggtccgggcc tcaaggacgc ggacatagga ggagcgcacc tcgaggtcgg
     4861 gccgcagcaa gagctggcct tcgtcggcgt cgaccaccgc ctcgtcaccc ggcccggtgg
     4921 cgtccagcat gccgcggaca ccgccgacgg ccggaaggcc gagcgcgcgc gcgaggatcg
     4981 ccgcatgccc gacagggctg gcttcctcca ccgcgatgcc cccgataccg gcggcgtgcc
     5041 agtcgagcag ttcggcgggg cccaggcgtc gggccagcag gatcgctccc ggcggcacgg
     5101 tcgggcgcgg tccatcgccc gccagcaccg cgagcagccg cgcggcaaga tcctccagat
     5161 cggagagccg ctcgcgcagg taggggtcgg tgatccgccg catccgatcg tgcagctcgc
     5221 cagtcacccg ctggaccgcg gcctccgccg acaggccact ctcgatcacg tcggagacac
     5281 gcttcatcca cccggcgtcg gcagcgatca gccggtaggc gtccagcacc tcccgggact
     5341 ccgccgtgtc ggcgccgctg acgaacccat ccggcgcgcc acggatcagc tcatccagtc
     5401 cctgttgcat gcgctggacg gcatcattca acctggccag ctcagcatcg gggtcgtcag
     5461 ccagcaggtg aagcggacgc cagcgccggc cgagcagcac aacctgtccg atggcgatgc
     5521 cgggtaccag cggcgcgccg gcaaacaccc gaggcaccgt ggcgccgaca ccctcctcgg
     5581 caccgtcggc cgcgccggtg gtgccaagca cctcggccag cagcatggcc actgtctcga
     5641 gctcgtccac ctcctcgtcg gagtagcccc gtgggttccg gttctgcacg gcaacgacgc
     5701 caagcgtgcg gccggcgcgg cgcaccggca cggcgagcat ggaggcgaac ggctcctcgc
     5761 cggtctcggg ccggtaggcg aaggcagggt gattctgggc gtccggcaaa ttcatcacct
     5821 gcgccgtggc ggcacacagg ccgatgattc cctcgcccac acgcaaccgc gtgcgaccga
     5881 cggcggccgg gttcaggccc tgggtcgcca cgagctccaa gatctcacca gggcgggcgg
     5941 catagatgga gcagacttcg ctgaccagtt ccgatgcgac aagctgcgca agttcctgca
     6001 acggcgcagt cccgtgcgcc attgtctcgc gcaatctggc aagtagtcgc cgcggggtcg
     6061 ccatcgccgg ctaacggggg cgcgcggcca gtgccgccac tgcctgccct accagttcct
     6121 ggatgatctc tgccgtcggt tgctccgccg tgaccatgcc gacggactgg ccggccatta
     6181 ccgaaccatt ctccacgtcg ccgtcgatca ccgcgcggcg cagggcgccg gcccagaaat
     6241 gttcgatcgc gagttgagcg gcttccttgg tgagttggcc agcctcgaag cggtcccgcg
     6301 tctcggcctg gaagcgcagg aagcgtttca cgccttcgtt caccagaccg cggactggga
     6361 tgaccggaaa gcgctcatcc aactggatcg agggaacagc atcgcgagcg gcagcgcgga
     6421 tgaacgcgcg cttgaaattg gaatgcgcga tcgactcact ggcggctgcg aagcgcgtgc
     6481 ccagctgggc gcccgacgca cccatttcga gcaacgtgag gatcgcctcg ccacggccga
     6541 ggccgccggc gacgaatacc ggcacgtccc ggatatgcgg caggatctcc tgagccagaa
     6601 ctatcagcga caccggcccg atatggccgc cggcctcgga accctcgatc accaaagcat
     6661 cggcgccgga tcggaccaat cgcttcgcca gcaccagtgc cggtgtgaaa gctatcaatc
     6721 gtgccccgcc ggctttcacc gcgcgcactg ccccgccggg cgggatgccg cccgccagca
     6781 cgatatggcc tacccgcgct tcgaggcaga cgcggatcag gtcgtccaaa cgcggatgca
     6841 tggtgatcag gttcacgccg aatgggcgtt cggtcagcgc ctgtgttgca gcgatttcgg
     6901 cggccagcat gtccggctcc atcgagccac acgcaatgac gccgaagccg ccggcgttgg
     6961 agatcgccgc gaccaggtgc cgctcgctga cccagctcat tgcgccgccc atgatggcgt
     7021 ggcggcagcc gaggaattcg gtgccgcgcg accataggcg gttgagctcg gcctctgccg
     7081 ctaccgtggc gcccgggatg gcaatgtcag gcggcatcga ggccataggc agtatgcagt
     7141 gcgcgcaccg cgagctcggt gtactcggcg gcaatcagca cgctcacctt gatctcgctg
     7201 gtggatatca c
//
LOCUS       DATGEM010000528         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1382974, whole genome shotgun sequence.
ACCESSION   DATGEM010000528 DATGEM010000000
VERSION     DATGEM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382974"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..513
                     /locus_tag="VKQ27_16170"
     CDS             25..513
                     /locus_tag="VKQ27_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90519.1"
                     /translation="MVDFIIETLGREQLAAVYPLVREAVPSLSLTSWLRFARPLTGSR
                     RSTHCGIVTARREGRSFPCGLFCFRVDDDLALGRVLVADHFVAVDLLDPGAVLTALVE
                     ELDRLAHRLECKAVRSLVHGGIRDVADSLDAAGHRPEDASLLLKRLSRAPAGGSRAAA
                     VA"
     gene            587..1882
                     /locus_tag="VKQ27_16175"
     CDS             587..1882
                     /locus_tag="VKQ27_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HLY90520.1"
                     /translation="MSFTEASALAFAARIGRGPRMWLRELGPGLITGAADDDPSGIVT
                     YSQAGAAFGYSLGWSVILTLPFMVAVQEISARLGRVTGQGLAAALKQHAPRPVLVALV
                     GLLVIANVINLGADIGAMAAVTQLLFGGPQSLYALGVAVLCASAEIWLAYKRYVRLLK
                     WLTVSLLAYVALLFVAHVDWPAALVGAVVPVVSLDRDAVTTVLAVLGTTISPYLLFWQ
                     AAEEAEDEANEADPRPLREHPADARGQMQRIGVDTWSGMVFSNVVALAMVIGSAATLH
                     AHGVTNIDTAAQAASALRPIAGPLAEAIFALGIFGTGLLAIPTLAGSSAYAVGESLNW
                     TVGLSRRTIEARSFYAFIAGATLVGAAIVFSPIAPIKALFWSAVINGIVAGPLIAAMT
                     VLASLRSVMGTLVLPTWLRVLGWVTALLMVAGTVGLVAV"
     gene            1993..>2423
                     /locus_tag="VKQ27_16180"
     CDS             1993..>2423
                     /locus_tag="VKQ27_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90521.1"
                     /translation="MYHQFTRSEAENILRSSEGGATSHRGGRPGHAGSRHLLLSNAEL
                     LERYTNFSEQLVWRNGRRRRREYTLITAFCTLPEMIDAAVLVLNAAQTQRALADFFTN
                     TPRGPGMRAEIAFTSAASFLMRYAQGEETVRTMPVSELVMIL"
BASE COUNT          349 a          652 c          953 g          469 t
ORIGIN      
        1 gacggacgga cagggtgagc gacgatggtc gacttcatca tcgagacgct tgggcgggaa
       61 cagcttgcgg cagtttatcc gttggtgcgg gaagccgtgc caagtctgag cctgacaagc
      121 tggctgcggt tcgcccggcc gctaaccggg tcgcggcgta gcacgcattg cgggatcgtg
      181 acggcgcggc gcgaggggcg gtcgttcccg tgtgggctgt tctgcttccg ggtggatgat
      241 gatctggcgt tggggcgggt gctggtggcg gatcatttcg tcgcggtgga cctgctggat
      301 cccggagcag tgctgacggc gctagtcgag gagctggaca ggctggcgca ccggctggag
      361 tgcaaggcgg tgcgcagtct ggtacatggc gggatacgcg acgtggcaga cagcctggat
      421 gcggcgggac acaggccgga ggatgcgtcg ttgctgttga agcggctgtc tcgggcgccg
      481 gccggcgggt cacgcgcggc agctgtggcc tgacgtctgt tgcgattgcg gcgcggccgg
      541 cgggcgcggt attcgcgcgc ctgccactac tagggagtcg ccgccgatgt cgttcaccga
      601 ggccagtgcg ctggctttcg cggcgcgcat tggacgcggc ccgcggatgt ggctgcggga
      661 gttggggccg ggtctgatta ccggggcggc ggacgacgat ccgtcgggaa ttgtcacata
      721 ttcgcaggct ggcgcggcgt tcggttactc cctgggatgg tcggttatcc tgaccctgcc
      781 gttcatggtg gcggtgcagg agatcagtgc gcgactaggg cgagtgacgg ggcaggggct
      841 ggcggcggcg ttgaagcagc acgcgccgag gccggtgctg gtggcgctgg tgggattgct
      901 ggtgattgcc aacgtcatca acctcggggc cgatatcggg gcgatggcgg cggtgacgca
      961 gttgctgttt ggcgggccgc aatcgctgta tgcgctggga gtggcggtgc tgtgcgccag
     1021 tgcggagatc tggctggcct acaagcgcta tgtgaggttg ctgaagtggc tgaccgtgtc
     1081 gctgctggcc tatgtggcgc tgctgttcgt ggcgcatgtg gattggcctg ccgcactggt
     1141 gggggcggtg gttccggtgg tgtcgctcga ccgggacgct gtgaccacgg tgctggcggt
     1201 gctggggaca acgatcagcc catacctgtt gttctggcag gcggccgagg aggcagagga
     1261 cgaggcgaac gaggctgatc ctcgtccgct gcgggaacat ccggcggatg cacgcgggca
     1321 gatgcagcgg atcggggtgg atacctggtc ggggatggtg ttctccaacg tagtggccct
     1381 ggcgatggtg atcggcagtg cggcgacgct gcacgcgcat ggcgtgacga atatcgacac
     1441 tgcggcacag gctgcctcgg cgctgcggcc gatcgcgggg ccgctggcgg aggcgatctt
     1501 cgcgctgggg atattcggca ccgggctgct ggccattccg acgctggccg ggtcttccgc
     1561 ttacgccgtg ggcgagtcgt tgaactggac ggtagggttg agccggcgga cgatcgaggc
     1621 acgatcgttt tacgcattca tcgccggggc gacattggta ggggcggcga tcgtgttctc
     1681 gcccattgcc ccgatcaagg cgctgttctg gtcggcggtg atcaacggga tagttgcggg
     1741 tccgctgatc gcggcgatga cggtgttggc gtcgctgcgc agcgtcatgg ggacgctggt
     1801 gctgccgaca tggcttcgtg tgctcggctg ggtaaccgcg ctgctgatgg tggcggggac
     1861 ggtggggctg gtggcggtgt aacacacggc caaatgattg acggatgccg cggccgcctc
     1921 gttggccgtc ttcctgccag tcctcctggg aggctaatta acgggtgcgg gcgcgccgaa
     1981 cggaggagcc ggatgtatca tcaattcact cgctccgagg ctgaaaacat cctgcgcagc
     2041 tccgaaggcg gcgcgacgtc gcatcgtggt ggccggccgg ggcacgcggg ttcgcggcat
     2101 cttctgctga gcaatgccga actgctggag cgatatacaa acttttcgga gcagctggtc
     2161 tggcgcaatg gcaggcggcg gcggcgcgag tatacgctga tcactgcatt ctgcacattg
     2221 ccggagatga tcgacgcggc cgtgctggtg ctgaacgcgg cacagacgca gagggcgctg
     2281 gccgattttt tcaccaatac gccacgtggg cccggaatgc gcgcggagat tgcatttacc
     2341 agcgcagcct cgttcctcat gcgctacgcg caaggtgagg agacggtgcg gacgatgccg
     2401 gtcagcgagc tggtgatgat act
//
LOCUS       DATGEM010000529         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_470908, whole genome shotgun sequence.
ACCESSION   DATGEM010000529 DATGEM010000000
VERSION     DATGEM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470908"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1800
                     /gene="selD"
                     /locus_tag="VKQ27_16185"
     CDS             <1..1800
                     /gene="selD"
                     /locus_tag="VKQ27_16185"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HLY90522.1"
                     /translation="VPFDLLSIDIGGEPAMPPGAGLPVKPIGQFLARLAALEAELSPG
                     ARIAVVGGGPGGTELALALAQRYRERARIVLVCDTPEPLAGAPSYARSIVRTALVNAN
                     VEFACGVSALDLADGRLALSDGSFLEVSTALWATGVVGPAILAAAGLACDAAGCIRVD
                     VTLRSLSHDFVFAAGDCAAIEGSARPKAGVWAVRAGPPLAANLRRAARGKPLRGWRPQ
                     KNALAILGLGDDSALAWRNGLAISGKPIWYWKDYIDRRWMRMYQEPMAAMGEADPMRC
                     GGCGAKVGAEVLTGALAALPLLPGRDVLIGLDAPDDAAVMLPPPGMAVVQSVDHFRAF
                     LDDPFVFGQIAAAHALSDLHAMGALPWTALAVAAIPYTTSSAKMRTELADMLAGATRI
                     LAADGCSLVGGHSAEAAEPALGFAVTGLAEPTQLLRKSGLRLGDALILTKPLGTGIIL
                     AAHMRGLARSACLQAAIASMCQSNAAASRILRAHGAIACTDITGFGLAGHLMEMLHAS
                     SVAATLWADQIPSLPGARELAAHGVESTLAPENRRVLDGYPLLADPQTSGGLLAGIAA
                     DRADACVAELRDAGAPAAIIGQVDAGNPAIRLV"
     gene            complement(1818..>3423)
                     /locus_tag="VKQ27_16190"
     CDS             complement(1818..>3423)
                     /locus_tag="VKQ27_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY90523.1"
                     /translation="VDDNADMRDYVEGLLGAQYVVRTAEDGEAALVEMRRERPDLVLS
                     DVMMPRLDGYGLLAAIRAEPGFRDLPVILLSARAGDEARVDGLDAGADDYLVKPFASR
                     ELMARVRANLELARLRREAADQLRSLNENLERRVAQEIAERMRAEEALRQAQKMEAVG
                     QLTGGVAHDFNNLLTAICGGADTLRRLLPEQLGPEEARLRRAVRMIEDGAQRAATLTQ
                     RLLAFGRRQALDPRPLDANKLVSGMSELLRRTLGEVVGIEIVLAGGLWRTMADPNQLE
                     SALLNLAVNARDAMPGGGRLTIETANTFLDEAYSATHPEVLPGQYVLIAVSDTGTGMQ
                     PDIVERVFEPFFTTKEVGQGTGLGLSQVYGFIKQSRGHVKIYSELGQGTVVKLYLPRL
                     IADTSAVEERDGVEAEAQMGSGELILVVEDDEDVRGYSVEALRELGYRVVAAANGTTG
                     LELLARYPAIRLLFTDVGLPGGMTGRQLADQARMRRPDLQVLFTTGYARNAIVHGGVL
                     DPGTHLLPKPFTLAALAAKVTGMLGE"
BASE COUNT          535 a         1354 c          962 g          572 t
ORIGIN      
        1 gtccccttcg acctgctctc catcgacatc ggcggcgagc ccgcgatgcc cccgggtgcc
       61 ggccttccgg tgaagccgat cggccagttc ctggcacgcc tcgccgcgct ggaagccgaa
      121 ctctcgcctg gcgcccgtat cgccgtggtt ggcggaggcc caggcggcac ggaactggcg
      181 ctggctctcg cgcaacgcta ccgcgagcgc gcccgcatcg tcctggtatg cgacaccccc
      241 gaacccctcg ccggcgcccc gtcctatgcc cgttccatcg tccgcaccgc cctggtgaat
      301 gccaatgtgg aatttgcctg cggcgtcagc gcgctcgatc tggcggacgg tcgcctcgca
      361 ctctccgacg gcagcttcct ggaagtctcc actgctctct gggccaccgg cgtggtcggc
      421 ccggcaatac tcgccgccgc tggcctggct tgcgacgctg ctggctgcat ccgcgtggac
      481 gtcaccctgc gcagcctcag ccacgacttt gtcttcgccg ccggcgactg cgccgcgatc
      541 gagggcagcg cacggcccaa agccggcgtc tgggcggttc gcgccggtcc tccgctcgcc
      601 gccaaccttc gccgcgccgc ccgcggcaag cccctccgcg gctggcgccc tcagaagaat
      661 gcactcgcca tcctgggtct cggcgacgac agcgctctcg cctggcgcaa tggcctcgcg
      721 atctcaggca agccgatatg gtactggaaa gactacatcg accgccgctg gatgcgcatg
      781 taccaggagc cgatggcggc catgggcgaa gcagatccca tgcgttgcgg cggctgtggc
      841 gccaaagtcg gggccgaggt tctgaccggc gctctcgccg ccctccccct ccttcccggc
      901 cgcgacgtgc tcatcggcct cgatgccccc gatgacgcag ccgtcatgct ccccccgccc
      961 ggcatggcgg tcgtccaaag cgtggaccac ttccgcgcct tcctcgacga cccgttcgtg
     1021 ttcggccaga tcgccgccgc ccacgctctc tccgacctgc acgccatggg tgctctcccc
     1081 tggacggcgc tcgccgtcgc tgcgatcccc tacacgacca gcagcgccaa gatgcgcacc
     1141 gagcttgccg acatgctcgc gggcgccacc cggatcctcg ctgccgacgg ctgctccctc
     1201 gtcggcggcc acagcgccga ggccgccgaa cccgccctag gcttcgccgt caccggcctc
     1261 gccgaaccca cgcagctgct gcgcaagtcc ggcctccgcc tcggcgacgc acttatactg
     1321 accaaaccac tcggcaccgg catcatcctc gccgcccaca tgagaggcct cgccaggtcc
     1381 gcgtgcctcc aggccgccat cgcctcgatg tgccagagca acgcggccgc ttcccgcatc
     1441 ctgcgtgccc acggagcgat cgcctgcacc gacatcaccg gcttcggcct cgccggccat
     1501 ctgatggaaa tgctgcatgc ctccagcgtc gccgcgacgt tgtgggcaga tcagatcccc
     1561 agcctgcccg gcgccagaga gctcgcggcc cacggcgtcg aaagcaccct cgcgccggaa
     1621 aaccgccgtg tactcgatgg ctaccccctg cttgccgacc cgcaaacctc gggtggcctg
     1681 ctcgccggca tcgccgccga tcgggccgac gcatgcgttg cagagctacg cgacgcggga
     1741 gcaccggcag ccatcatcgg ccaggttgat gccggcaacc ctgcaatccg cctcgtctag
     1801 gctgaattct gccgctccta ctcccccagc atcccagtca ccttggcggc gagcgccgcc
     1861 agcgtgaacg gcttcggcag cagatgcgtg cctggatcga gaactccccc atgcacgatg
     1921 gcattacgcg cgtatcctgt ggtgaacagc acctgcagat caggccgccg catccgcgcc
     1981 tgatcggcca attgccgccc tgtcattccg cccggcaggc ccacatccgt gaacagcagg
     2041 cggatggcag gataccgcgc cagcaactcg aggcccgtcg tgccgttcgc tgccgccacg
     2101 acgcgatagc cgagttccct cagtgcctcc acactgtaac cacgcacgtc ttcatcgtct
     2161 tccaccacca ggatcagttc gcctgaaccc atctgggctt ctgcctcgac gccgtcgcgc
     2221 tcctccaccg cgctcgtatc ggcgatcaga cgtggcaaat acagcttcac caccgttccc
     2281 tgcccaagtt cgctgtatat cttgacgtgt ccacgcgact gcttgatgaa cccatacacc
     2341 tggctaagtc ccagaccggt tccctgtccg acctccttgg tggtaaagaa cggctcgaac
     2401 acgcgctcca cgatgtccgg ctgcatgccc gtcccagtat cggacaccgc gatcagcaca
     2461 tactgccctg gcagcacctc cggatgcgtc gcagaatatg cctcgtcgag gaacgtgttt
     2521 gccgtttcta tcgtcaaacg cccgccgccc ggcatcgcgt cgcgcgcatt cactgccagg
     2581 ttcagcagcg cgctctccaa ctggttcggg tcggccatcg tccgccacaa gccgcctgcc
     2641 agcacgatct cgatccccac cacctcgccc agcgtacgcc gcagcaactc cgacatgccc
     2701 gacaccagct tgttggcatc gagcggccgg ggatccagcg cttgccgtcg cccgaatgcc
     2761 aacagccgct gcgtcaatgt cgcggcacgc tgcgcaccgt cctcgatcat gcgcaccgcg
     2821 cgccgcagcc gggcttcctc cggaccaagc tgctcgggca gcagacgtcg caacgtatcg
     2881 gcgccgccac agatcgccgt cagcagattg ttgaagtcgt gcgcgacgcc gccggtgagt
     2941 tgccccaccg cctccatctt ttgcgcctgc cgcagggctt cctcggcgcg catgcgctcg
     3001 gcgatttcct gcgcaacccg gcgttccaga ttctcattga gactccgaag ctgatcggct
     3061 gcctcacgcc gcagccgcgc cagttccagg ttggctcgca cccgcgccat cagttcgcgt
     3121 gaagcgaatg gcttcaccag atagtcgtcc gcgcctgcat ccagcccgtc cacccgagcc
     3181 tcgtcacccg ctcgcgctga aagcaggatc accggcagat cacgaaaccc aggttccgcc
     3241 cggatcgccg ccagcaagcc atatccatcc agccggggca tcatcacatc actcaacacg
     3301 aggtcaggtc gttcgcgccg catttccaca agcgccgcct cgccgtcctc cgccgtccgc
     3361 acaacgtact gcgcacccag cagcccttcg acgtagtcgc gcatgtccgc attgtcgtcc
     3421 aca
//
LOCUS       DATGEM010000530         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_403645, whole genome shotgun sequence.
ACCESSION   DATGEM010000530 DATGEM010000000
VERSION     DATGEM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403645"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1347
                     /locus_tag="VKQ27_16195"
     CDS             130..1347
                     /locus_tag="VKQ27_16195"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HLY90524.1"
                     /translation="MLISRIAVARPLRLLANLLLAAALSSGCGGGGGSDSNAPARSSF
                     TAGPISGFGSIIVNGIRFDVSRARVVDDEGRVRTEADLKLGMMAQVQAGDITSSNTGQ
                     ATQVQIGSVIIGPVDAINTSTRLLIVLGQTIDVADTTVFGPSLPSGPFSISVGTVLEI
                     FGMLDTTTGHYQATRIEANTDATFFKLQGLIANLDSAARTFMIGGETISFASLAAGSV
                     PPLSDGKQVTATLQMQPDRNNDWIAIQLTTDASAPPAAQSKVKLRGFIASVDSPGHFT
                     INGLPVDASNAAIHPKNAALVPGAQVKVEGSISNGTVIATEVTVKKGRGHGSENDENE
                     DEDDQFELHGAVENLNTSSQTCLVRGVPVDFSAAVFSGGRSQLASGSRINVKGKLSSD
                     GTRVDASSISFED"
     gene            complement(1375..2553)
                     /locus_tag="VKQ27_16200"
     CDS             complement(1375..2553)
                     /locus_tag="VKQ27_16200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90525.1"
                     /translation="MTDTRLTTMLLALCTATFFVTSAGASMAPFLNLIALSLGTTLPD
                     VAHLFSIQALAWGIASLVVGMVSGRFGRRALLVSGVLLMGVMRLGFATSHSYAAAVLW
                     QVLSGIGGGTFMGVVYAAVSEHAAAGMRGRAMSWVITGQSLSLVLGVPLVTLLGAFGG
                     WRGAIATHGALVLSCALAVRLATPGDPPAVAHEHRPKTPYMELFKPKLVALLAAGTTE
                     RMCFAVVAIFLPAFLQDAYGTSLGALALVLALVAAGNLAGNILGGRIADRTRFRARMF
                     AASSALTAVLALPLLGWHPGLLPSVVLGFVYSFVNAAGRPSLMARLAEVPGELRSALF
                     GLNITMASLGWLLAGSVGAGLIALGGFAGLGAFCSSVAALGCLLALYSAGKTPRRQIA
                     "
     gene            complement(2733..4247)
                     /locus_tag="VKQ27_16205"
     CDS             complement(2733..4247)
                     /locus_tag="VKQ27_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY90526.1"
                     /translation="MVQYPTPEQIWDAAEEVGLELTEDDVKSYLGLIKPNIDAYNLVD
                     AMPDYLPAVKYPRTPGYFPDPSENTRNAWYVRTSIKGASSGPLKGKKVAVKDNVMVAG
                     VPMMNGNSILEGYVPDIDATVVARLLDAGAEIIGKTHCESYCISGGSHTGAKGAVHNP
                     HKMGFSAGGSSSGSGVAVALGEADMALGGDQGGSIRMPSSWCGIYGMKPTHGLVPYTG
                     IMPIEVYVDHTGPMTATVADNALMLEVIAGPDGYDPRQYGAPKKLKYSKMLDGGAQGL
                     KIGVVKEGFGRPESEAASDAKVRKAVDLYRKLGAQVEDVSIPAHLIGPALWTPIGVEG
                     ITQTMMFGDGYGLSRPDLYVTSLIDKLHGWELRANELSETTKVLTTLGVYIKKYHGSH
                     YYGKAMNITRRLIEAYDAQLARYDLLLMPTMPMKAQPLPAPNCSREEYFQRALEMLGN
                     TCPFDITHHPAMTVPCGFVDGLPIGLMLIGKHYDEATIYRAAYAFEQTGDWKKM"
BASE COUNT          916 a         1356 c         1368 g          780 t
ORIGIN      
        1 atcaggcaat cgcggggcat gactattttt ttccgttcat cccaagtgca tgcttgcggg
       61 aatccaaatc ccgtcgagtg attcacgcca cgaatcagcg gtcggctttg gcaacaaagg
      121 agttccgcaa tgctgatatc tcgaattgcc gtagcccgcc cgcttcgtct actcgcgaac
      181 ctgcttctcg ccgccgcact gtcgagcggc tgtggcggag gcgggggctc ggattcaaac
      241 gcgcccgcca ggagttcatt taccgcgggt ccgatctccg gtttcggatc gattatcgtc
      301 aatggcatcc gcttcgatgt ctccagggcg cgagtggtcg acgacgaagg ccgcgtgcgc
      361 actgaggccg atctgaagct cggcatgatg gcgcaggtgc aggccggcga catcacgagc
      421 agcaataccg gccaggccac gcaggtccag atcggcagcg tcatcatcgg gccggtcgac
      481 gcgatcaaca cctctacgcg gctgttgata gttcttgggc aaaccatcga tgtagcggac
      541 accaccgtat ttggtcccag cctgccgagc ggacccttca gtatttcggt gggcaccgta
      601 ctggaaatct tcgggatgct cgacacgacg acaggccact atcaggccac gcgcatcgag
      661 gccaacaccg atgcgacatt tttcaagttg caaggcttga ttgccaacct cgactccgca
      721 gcgcgcactt tcatgatcgg cggcgaaacc atatcgtttg ccagtttggc cgcgggcagc
      781 gtcccgccgt tgagcgatgg caagcaggtc accgccaccc tgcaaatgca gccggatcga
      841 aacaatgatt ggatcgccat ccagctcacg accgatgcga gtgctcctcc ggccgcgcag
      901 agcaaggtga aactcagagg attcatcgcc tccgtcgatt cgcccggtca cttcactatc
      961 aacggtctgc cggtggacgc cagcaacgcc gcgatacatc cgaagaacgc agcgctggtg
     1021 ccgggcgcgc aggtcaaggt cgagggctcg atcagcaatg gcacggtcat cgccaccgag
     1081 gtgacggtca agaaaggccg gggccacgga tccgaaaacg acgagaacga agacgaggac
     1141 gatcaattcg aattgcacgg ggcggtcgaa aacctgaata cgtcttcgca gacctgcctg
     1201 gtacgcggcg tgcctgtcga tttcagcgct gccgtgttca gcggcgggcg cagtcaactc
     1261 gccagtggca gcaggatcaa cgtcaaaggc aagttgtcct ccgacggaac acgggtggac
     1321 gcctcgagta tcagtttcga agactaaagg ggcggcggcc gggcggcctg acaatcatgc
     1381 gatttggcgc cggggtgttt tcccggcgct gtatagcgcc agcaagcaac cgagcgcagc
     1441 aacggatgag cagaaggcgc ccagcccggc gaaaccgccc agcgcaatca atcctgcgcc
     1501 gacggagccg gccagcagcc agccgaggct cgccatggtg atgttcaagc cgaagagcgc
     1561 gctgcgcaac tcgccgggca cttcggccag cctggccatc agcgagggcc ggccggcggc
     1621 attgacgaag gaatacacga agcccagcac gacggaaggc agaagccccg gatgccagcc
     1681 gagcaagggc aacgccagca cggccgtcaa tgcggaactc gccgcgaaca tgcgcgcccg
     1741 gaagcgcgtg cgatcggcaa tgcgcccgcc cagaatgttg ccagcgagat tacccgcggc
     1801 gaccagggcc agcaccaatg cgagtgcgcc gagcgacgtg ccgtacgcgt cctgaaggaa
     1861 cgcaggcaaa aaaatggcga cgacagcgaa gcacatccgc tcggtcgtgc cagccgccag
     1921 cagcgccacg agtttcggct tgaacagttc catgtagggt gtcttcggcc gatgctcatg
     1981 cgcaacggcg ggcggatcgc caggcgtggc cagccgcacg gcaagcgcgc aggacaaaac
     2041 caatgcgccg tgggtggcga tggcgccgcg ccacccgccg aaggcaccga gcagcgtcac
     2101 cagcggaacg cccagcacca gcgacagcga ttgaccggtg atgacccagc tcatcgcgcg
     2161 cccgcgcatt ccagctgcgg cgtgttccga caccgcggca tagaccacac ccatgaaggt
     2221 gcctccgccg atgccgctca acacctgcca caacacggcg gcggcataac tgtgcgatgt
     2281 ggcgaagccc aggcgcatca cgcccatcaa caagacgccg ctcaccagca gggcacggcg
     2341 gccgaagcgc ccggaaacca tgcccacgac gagcgaggcg attccccaag ccagagcctg
     2401 aatggagaac aggtgggcaa cgtcgggcag cgtggtgccc agactcagcg caatcaaatt
     2461 cagaaaaggc gccatgctgg cgcccgccga ggtgacgaag aaagtcgccg tgcacagggc
     2521 gagcagcatc gtcgtcaggc gggtgtcggt catcgaagcg gtgaacggtg aatggtgaac
     2581 agtgaaccgc aaacactaac agcgaaaaga cgaatgatga gcaatcagca gaaaaaaggg
     2641 aaccgcatcc cgattccctt tccgttcaac acgacttaaa tcagactctc tcgatcaccg
     2701 atcaccgatc accgatcacc gatcaccgat cattacatct tcttccagtc cccggtctgt
     2761 tcgaacgcgt atgcggcgcg atagatcgtc gcttcgtcgt aatgttttcc gatcagcatc
     2821 agcccgatcg gcaatccgtc gacgaaaccg cagggtacgg tcatggcggg atggtgggtg
     2881 atgtcgaacg ggcaggtgtt gccgagcatc tcgagcgcgc gctggaagta ttcctcgcgt
     2941 gagcagttcg gcgcgggcaa aggttgcgcc ttcatcggca tggtgggcat cagcagcaga
     3001 tcgtaccttg caagctgcgc atcgtaggct tcgatcagac gcctggtgat gttcatggct
     3061 ttgccgtagt aatggctgcc gtgatatttc ttgatataga caccgagcgt ggtcagcacc
     3121 ttggtggttt ccgacaattc attggcgcgc agctcccagc catgcagttt gtcgatgaga
     3181 ctggtcacgt agagatcggg gcggcttaag ccgtagccat cgccgaacat catcgtctgc
     3241 gtgatgcctt cgaccccgat cggggtccac agggcgggcc cgatcaggtg cgccggaatg
     3301 gacacgtctt cgacttgcgc gccgagcttg cgatacagat cgactgcctt gcgaaccttg
     3361 gcatccgagg cagcttcgga ttccgggcgg ccgaagcctt ccttgaccac gccgatcttg
     3421 aggccctgcg cgccgccatc gagcatcttc gaatacttca gcttcttcgg cgcgccgtac
     3481 tggcgcggat cgtagccgtc gggaccggcg atgacttcga gcatcagcgc attgtcggcg
     3541 acggtggcgg tcatcgggcc ggtatggtcg acgtacactt cgatcggcat gattccggtg
     3601 taaggcacca gcccgtgcgt tggcttcatg ccgtagatgc cgcaccacga ggagggcatg
     3661 cgaatggagc cgccctggtc gccgcccagg gccatgtccg cttcgccgag ggcgacggcg
     3721 acgccgctgc ccgatgacga accgccggcc gaaaagccca tcttgtgcgg attgtgtacc
     3781 gcgccttttg ctcctgtgtg gctgccgccc gaaatgcagt aggactcaca gtgcgtcttg
     3841 ccgatgattt cagcgccggc atcgagcagg cgcgcgacga cggtggcatc gatgtccggc
     3901 acatagcctt cgaggatcga gttgccgttc atcatcggca cgccggccac catgacgtta
     3961 tccttgacgg cgactttctt gcctttgagc ggtcctgagg aagcgccctt gatcgaagtc
     4021 cgcacgtacc aggcgttgcg tgtgttctcg gagggatcgg gaaagtagcc gggcgtgcgc
     4081 ggatatttga ccgccggcag gtaatccggc atcgcatcga ccaggttgta ggcgtcgata
     4141 ttgggtttga tgagcccgag ataggacttc acgtcgtcct cggtcaactc gagaccgact
     4201 tcttcggcgg cgtcccagat ctgttcgggt gtcggatatt ggaccatatg gcctcctcct
     4261 acggatggtt gttgtaaaga atccccgact tgatatcagg gcgcgagcaa gcggagcagt
     4321 tcgggatcgt cgcgcatctt gcgggattct ccgctcaacg caatgcggcc gcgatccata
     4381 acgtaacaat actgcgaaat gtcgagtgcg agcttcaggt
//
LOCUS       DATGEM010000531         5629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1694754, whole genome shotgun sequence.
ACCESSION   DATGEM010000531 DATGEM010000000
VERSION     DATGEM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694754"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..995
                     /locus_tag="VKQ27_16210"
     CDS             216..995
                     /locus_tag="VKQ27_16210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90527.1"
                     /translation="MERFYEGAESLFVRAYDAFYREVPPQMAGDVAFYRQLAQRTGGP
                     LLELACGTGRIAIPLAESGLDVTGVDVSDGMLAIIRRKATAHPVTLIAQDMSILQLDR
                     QFGCIIVAFRSFQHLLTIELQRQTLAAIHRHLAPNGRVALNLFDPRLDLLIDETPRQA
                     GLRGTDPITGHTFTGELLSARFDYLAQIRHDLWRYREYAPDGVLLAEDTRDMALRWTW
                     RWELHHLLELCGFAVEAQYSDFTGSPPAYGKELIVIARMRD"
     gene            1044..3410
                     /locus_tag="VKQ27_16215"
     CDS             1044..3410
                     /locus_tag="VKQ27_16215"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLY90528.1"
                     /translation="MSARGMDGASPIQAYLAGTIGEDELLVQVDRVLADGSVIDRAAL
                     LDDWRTKSGRIRASGIRDQLSARVQALAWSGSDVDDTMNGPSPRSRELKAGDVLAHRF
                     VIEEKIGSGGMGSVFKARDLRREEAQDRHPYVAVKTLNVDLLQREDSLKILQREARKA
                     QSLSHPNIVRVYDFDRDGQTMFVTMELLEGLSLEAMIRANGLTGTTLQETLPILGQVA
                     SALEFAHAEGIVHSDLKPANILVLNNGRVKVIDFGIARAVPTPGQLTMDRTTFDIQAL
                     GALTPAYASPEMIEGLDPDPRDDAFAFACITYELLTGMHPFGRTPALAARAANLRPKR
                     PARLSASQWRALQSGLELERTKRAASPARLLAAMQDKPAIWRRRGFAIQAGVGVVVIA
                     ALVGLLAHFAHQPGSAPERTAAPKAADQPQPGEREAGKSPAQQQADEAAQRLAQQRAE
                     EAAAQRAAQQQARQQADEESARRLAQLQADEAAARRLAQQQTDAAAKPPVQPPSAEAL
                     VPADIAELQRLLNAIGLNVGAPDGKAGPRTQEMARAFQLATGEPGNGELTPGLLGALR
                     QAKPSADAKAKAVFSLATAASRSGRTGEAIRLYELGLTFAPVNSDALLALGGLYRDKN
                     DYDAARRQYELVPKNSGSSAEAARQQLAALPRQQQLPASQTGPATLDASAPGMPAGSA
                     ATQATGRAGSDRPLDGLYAGTSQVSGYSSPNCIAGQMRLEVRNGRLLLGGDRQITVAS
                     DGNFDGTTPIGHPPVAQLWTGKIGADGMEADVKDPACRFHISLKKVSR"
     gene            complement(3504..4730)
                     /locus_tag="VKQ27_16220"
     CDS             complement(3504..4730)
                     /locus_tag="VKQ27_16220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLY90529.1"
                     /translation="MRILMLLFLLLPWPAWAAADPVFSDTGPEDVAYGANAGYPTRRG
                     GALLPQMYMVGDYSRFDTLWPSHVVAKSSSPSSLRRADNELALTYQYAGGSYTLDDYL
                     RRNPVTGLLIARDDTILFEHYQYDRSDRDRMLSQSMAKTITAMLLGIAVSEGAIRSID
                     QPAADDVPALADTEYGRTSIRNLLHMASGVAFHEVYDGADDSAKLGLMLFVRGVPPEQ
                     AVAQFNTREAPPGTRFHYAGAETETLGQVVSNAVRMPLAQYLQSRIWQPMGAEADATW
                     VVDGTGHEVAYCCFNATLRDWARFGLILAHDGAWNGRQIIPRQWVLDATSVAAPYLAP
                     GVATRGDGYGYQIWLLPGGRRAFALRGIHGQVILIDPTLHLVMVHMAVRLKASADPAA
                     AEMAALWHALVAHFGG"
     gene            complement(4751..>5629)
                     /gene="hemW"
                     /locus_tag="VKQ27_16225"
     CDS             complement(4751..>5629)
                     /gene="hemW"
                     /locus_tag="VKQ27_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLY90530.1"
                     /translation="VTLEANPTSVEAGKFAAFRDAGINRVSIGVQSLDADDLRNLGRQ
                     HSVAQAIGAIEIARTAFPRISFDLIYARPGQTLATWRTELRQALGLAADHLSLYQLTI
                     EPGTQFATLYQRGALTLPDDETSTALYQATAEEAARSGLLPYEVSNYARPGGESRHNL
                     TYWRYGDYAGIGPGAHGRVTLSGGRLLAVRRHRAPEVWAERVEREGHGVVEETPLGAV
                     ERAREMLLMGLRLSEGIDAARFAARTGMELADALDAAVLEQAIAEGYLACREGRLIAL
                     AEGRLRLDALLAALVR"
BASE COUNT         1045 a         1886 c         1762 g          936 t
ORIGIN      
        1 cctgaccgga tgcgacgaca cgctgcgctc tacacagaac ggctcggcct ggatccgctg
       61 cgggtactgg gcttcgcctt tgtcggcggc gtggtcgctc gaggacagac gcgacccaac
      121 cttccgcctg cgatgcgccg ccgtcctcga gcttttggtt gattgacacg gcgcctcaca
      181 ccaacgttcg gtgaggctca ggcacggagt cgcggatgga acgcttctac gaaggtgcgg
      241 aatcgctgtt cgtccgcgcg tacgatgcat tctatcgcga ggtgccgccc cagatggccg
      301 gcgacgtcgc gttctaccgc cagctcgcac agcggaccgg cggcccgctg ctggagcttg
      361 cctgcgggac tggccgcatc gcgattccgc tcgccgaaag cggactggat gtcaccggtg
      421 tcgacgtctc tgatggcatg ctggcgatca ttcgccggaa agccacggca catcctgtga
      481 ctctgatcgc gcaggatatg agcatattgc agctcgatcg gcagttcggc tgcatcatcg
      541 tcgccttccg ctcattccag catctgctga cgatcgaact gcaacgccag accctggcgg
      601 cgatccaccg ccatctcgct ccaaacggcc gcgtcgccct caatctgttc gatccacgcc
      661 tcgacctgct gatcgacgag acgcctcgcc aagcaggtct ccgcggcacc gatcccatca
      721 ccggtcacac attcaccggc gagttgctga gcgcgcgctt cgactatctg gcgcagatcc
      781 gccacgacct ttggcgctac cgcgaatacg ctccagacgg cgtgctgctc gccgaggaca
      841 cgcgcgatat ggcgctgcgt tggacctggc gctgggaact ccaccacttg ctggaactgt
      901 gcggtttcgc ggtggaggcg caatacagcg acttcactgg ctcgccgccg gcatacggca
      961 aggaactgat cgtgatcgcg cgcatgcgcg actgatgggc gtgcggaact ccgccagttt
     1021 gcttgcaaca atgaggctgt ctgttgtctg ctcgcggcat ggacggcgct tctcccatcc
     1081 aggcctacct cgccggcact atcggtgaag atgagctgtt ggtgcaggtc gatcgggtgc
     1141 tggcggacgg atcggtcatc gaccgggcgg cgttgctcga cgattggagg accaagtccg
     1201 gccgaattcg cgcctccggt atccgtgacc agctcagcgc cagagtgcag gccctcgcgt
     1261 ggtcgggatc ggatgtcgat gacaccatga atgggccgtc gcctcgttcg agggagttga
     1321 aagccggaga cgttctcgcc caccggttcg ttatcgaaga gaagatcggc tccggaggca
     1381 tgggtagcgt cttcaaagcc cgcgacctgc gccgtgagga ggcacaggac cggcatccct
     1441 acgtcgcggt gaagacgttg aacgtcgatc ttctccagcg cgaggattcg ctcaagatcc
     1501 tgcagcgaga ggccaggaag gcccaaagcc tttcccatcc gaacatcgtg cgcgtctatg
     1561 attttgaccg cgacggccag accatgttcg tcaccatgga actgctcgag gggttgtccc
     1621 tcgaggcgat gatccgtgcg aacggcctga ccggtacgac gttacaggag acgctcccga
     1681 tactcggaca ggtggcatcc gcattggaat tcgctcacgc agagggaatc gtccactcgg
     1741 acctgaaacc agcgaacatc ctcgtcctga acaatgggcg cgtgaaggtg atcgacttcg
     1801 gcatcgcgcg agcagtaccg acgcccgggc agctcaccat ggatcggacc actttcgaca
     1861 tacaggcgct cggggcgctc accccagctt acgcgagccc ggagatgatc gaaggcctgg
     1921 atccagatcc gcgcgacgac gcgtttgcct tcgcttgcat cacctacgaa ctgctgaccg
     1981 gcatgcatcc gtttggccgg actccggctc tggcagcgcg cgcggccaat ctgcggccga
     2041 agcggcctgc acgtctgtcg gccagccaat ggcgagcgct ccagagtggt ctggaactcg
     2101 agaggacgaa acgcgcggca agtccggcac gccttctggc ggcgatgcag gacaagcccg
     2161 cgatctggcg aaggcgcggt ttcgcgatcc aggccggcgt tggtgttgtc gtcatcgcgg
     2221 cactggtcgg tctgttggcc cacttcgcgc atcagcccgg gagcgcgcct gagcggactg
     2281 ccgcacccaa ggcggctgac cagccgcagc ccggtgagcg cgaggctgga aagagtccgg
     2341 cccaacaaca ggcagatgag gcagcgcaac gcctggccca acagcgcgcg gaagaggctg
     2401 ccgcgcagag ggccgctcag cagcaggcgc gacagcaggc ggacgaggaa tcggccaggc
     2461 gcctggcaca actacaggcc gatgaggcag ccgccaggcg ccttgcccaa cagcagacgg
     2521 acgcggcagc gaagccacca gtgcagcctc catcggcgga agctttggtc cccgcggaca
     2581 tcgccgagtt gcagcggctg ttgaatgcca tcgggctgaa tgtcggcgcg cctgacggca
     2641 aggccggacc acgaacgcag gagatggcaa gagccttcca gctggccacc ggcgaaccag
     2701 gaaacggtga gttgacgccg gggctgctcg gggcattgcg tcaggcgaag ccgtcagccg
     2761 atgccaaggc caaggctgtg ttcagcctgg cgacagcggc aagccgttca ggacgaaccg
     2821 gcgaggcgat ccggctatat gaacttgggc tgacattcgc gcccgtgaat tccgatgcac
     2881 tgctcgcgct tggcggtctg tatcgcgaca agaatgacta cgacgcagcg cggcggcagt
     2941 atgagctggt gccgaagaac agcggctcgt cagccgaagc ggcgcggcag caactggcgg
     3001 cattgcccag gcagcaacag ttgccggcaa gtcaaaccgg gcctgccact ttggacgcat
     3061 ccgcgccagg aatgcccgcc ggttccgcgg cgacgcaggc cacgggacgc gccgggtccg
     3121 accggccatt ggacggcctc tatgccggga caagccaggt atcgggatac agttcgccga
     3181 actgcatcgc gggccagatg cgcctggaag tgcgaaatgg cagattatta ctcggcgggg
     3241 acagacagat aaccgtggcg agcgacggaa acttcgatgg cacgacgcca attggccatc
     3301 caccggtcgc gcagctctgg actggaaaga tcggggccga cggcatggag gccgatgtca
     3361 aggacccggc gtgcaggttt catatttcgc tgaagaaggt ttcgcgatga atggggcttc
     3421 gccgcccgcc tgccgcaaga aactcatcgt tgtggcgcga ccgcgcatcg cacaaccatc
     3481 ggcctctcga ggcacaaatc acatcatccc ccaaaatgcg ccacgagcgc gtgccacagc
     3541 gcggccattt ccgccgccgc cggatcggcc gacgccttca gcctcaccgc catatgcacc
     3601 attaccagat gcagcgtggg atcgatcagg atcacctggc catgaatgcc gcgcagcgcg
     3661 aatgcacgcc gcccgccggg cagcagccaa atctgatagc catagccatc gccccgtgtc
     3721 gcgacgcccg gcgccagata cggcgccgcc acgctggtcg cgtccagcac ccattgccgg
     3781 gggatgatct gcctgccgtt ccaggcgccg tcgtgcgcca agatcagtcc gaaacgagcc
     3841 cagtcgcgca gcgtggcgtt gaagcagcaa tacgccacct catgccccgt cccatcgacc
     3901 acccaggtcg catctgcctc cgcgcccatc ggctgccaga tccggctttg caggtactgc
     3961 gcgagcggca tgcgcaccgc attgctgacc acctggccga gcgtctccgt ttcggctcct
     4021 gcataatgaa atcgcgtccc aggcggagcc tcgcgcgtat tgaactgcgc aaccgcctgc
     4081 tccggcggca cgccgcggac gaacagcatc aggccaagct tcgcgctgtc gtccgcgccg
     4141 tcatagactt cgtggaacgc cacgccagat gccatgtgca gcaggtttcg tatagacgtc
     4201 cggccatact cggtgtccgc cagcgccggc acatcatcgg cggctggttg atcgatcgag
     4261 cggatcgcgc cctccgacac cgcaataccg agcagcatcg ccgtgatcgt cttggccatc
     4321 gattgggaca acatccggtc tcgatcgcta cggtcatatt ggtagtgctc gaacaggatc
     4381 gtgtcgtccc gcgcaatcag cagtccggtc accggattgc ggcgcagata gtcgtcgagc
     4441 gtgtagctgc cgccggcgta ctgatacgtc agcgccagtt cattgtccgc gcgccgtagc
     4501 gaggacggcg atgacgactt cgccacgaca tgcgatggcc acagcgtatc gaaccgactg
     4561 tagtcgccga ccatgtacat ctgcggcagc aaggcgccgc cgcggcgggt cggataccca
     4621 gcgttggcgc cataggccac gtcttccggc ccggtgtcgg agaacaccgg atcagcggca
     4681 gcccacgccg gccacggcag cagcaggaac agcaacatca gtatccgcat cagcggacct
     4741 ggccccgagc tcaccgcacc aacgccgcaa gcagtgcgtc cagccgcagc cgcccctccg
     4801 ccaacgcgat cagccgcccc tcgcggcacg cgaggtatcc ctccgcaatc gcctgctcga
     4861 gcactgccgc atcgagtgcg tccgccaatt ccatcccggt ccgcgccgcg aaccgagccg
     4921 catcgatgcc ctcggacaat cgcagcccca tcagcagcat ctcccgtgcg cgctcaaccg
     4981 cgccgagtgg cgtctcctcg accactccat ggccttcgcg ctcaacgcgc tccgcccata
     5041 cttccggcgc acgatggcga cgcacggcaa gcagtctgcc gccgctcaag gtcaccctgc
     5101 catgggcccc cgggccgatg cctgcgtagt cgccgtagcg ccagtaagtg aggttgtgcc
     5161 ggctttcgcc acccggacgc gcataattcg aaacttcgta gggcagcaat ccggatcgcg
     5221 cggcttcctc cgccgtcgcc tggtaaagcg ccgtggatgt ctcgtcatca ggcagcgtga
     5281 gagcgccgcg ttgataaagc gtggcgaact gcgtgcccgg ctcgatggtc agttgataaa
     5341 gtgacagatg gtccgctgcc agtccgagtg cctgccgcag ttcggtccgc catgtcgcca
     5401 atgtctggcc agggcgcgca taaatcagat cgaatgagat gcgcggaaac gcggtcctcg
     5461 cgatctcgat tgccccgatc gcctgcgcca cggagtgctg ccgcccgagg ttgcgcagat
     5521 catctgcatc gaggctctga acgccgatcg acacgcgatt tatgccggca tcgcggaacg
     5581 ccgcgaattt cccggcctct acgctggtgg gattcgcctc gagcgttac
//
LOCUS       DATGEM010000532         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1059235, whole genome shotgun sequence.
ACCESSION   DATGEM010000532 DATGEM010000000
VERSION     DATGEM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059235"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VKQ27_16230"
     CDS             complement(<1..1798)
                     /locus_tag="VKQ27_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY90531.1"
                     /translation="MLHNATGTGIGQPVRRREDLRLLTGRGRYSDDINVPGQAHAVMV
                     RSTHAHAVIRSIDTAVAATAPGVLAVLTGRDMLADGLKPIPHAVRTGHPADIALDNSD
                     GSPPFIPPHYAMATDEARHIGDIVAMVVATSRTAAKDAAEQVIVSYADLAAVVSSRAA
                     IAPNAPLTWSDARSNFCLDAQIGDAAATDAAFASAAHVVRLATRVQRIAGVTMEPRAA
                     TGEYDATTNSYTLHAGAGGAVRPRHDMAVVLGVPDDNVRMVMHDVGGNFGTRGASNPE
                     FALVAWAAKRVHRPVKWTCERSEAFLCDYQARDLTADAELALDANGKFLAMRGTNIVD
                     SGAYPVSFGPLHKGVEIMSSIYHVPTVHFRACATLTNTAPTRPYRSSGRPEAMFVVER
                     LIDIAARQCGIDRVELRRRNLVPESAMPYRNPFGLEYDSGAYHKVMETVLTLGDWQGF
                     SSRRDAARARGKLRGIGIANYVDTATGVPRERAEITVLPSGVVELVIGTVSSGQGHET
                     SFAQLVGEWLGVPMESVVLVQGDTARVSVGGGSHSGRALRLGSIVMLGATNEIIAKGL
                     RIAGHILEAAVADMEFSSGRFIVKGTDRSIGIF"
     gene            complement(1924..2925)
                     /locus_tag="VKQ27_16235"
     CDS             complement(1924..2925)
                     /locus_tag="VKQ27_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HLY90532.1"
                     /translation="MSEAVFVSKHLIARHAGSALWLARYMERIENLARLLDVTKTFAR
                     DDDSHRNWLSLLRINGDTGTFFATNQVADATSVARFYLLDGDNPTSVQSGIRYARENA
                     RTLRALISTEMWLQINVFHGQIRALTEADISPEEVSAVCAMLKEGVQSHTGITEGTFY
                     RDQCWHFYMMGRHIERADQITRLIDTKFNALMPKAADAAIDAGEWNELLRAAAGYHAY
                     RREYPHGYRPPEVAGFLLLNTAFPRSAGLSLAQLDWHLTTLRSRYHLRGCTPALARLD
                     ELQSMLAAQTVEEILRRGLSQFLDWMQRELGALHTDIMTRLCGAPVLVEQEQTQSQA"
     gene            complement(2922..4364)
                     /locus_tag="VKQ27_16240"
     CDS             complement(2922..4364)
                     /locus_tag="VKQ27_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HLY90533.1"
                     /translation="MQQDGVLAGYDPGALFCEMFRPGQPPHPALTLLRERLGELPIDS
                     LRRRSADAERDLLERGITFTVYSDATAIDRILPFDLIPRVITAEEWHTLESGVAQRVR
                     ALNLFLHDIYHDGKILKDGVVPRELVLGNAAYCQAMVGFSPPHKTYVHVCGIDIIRDD
                     SGRYLVLEDNARTPSGVAYVVENRHMTLRVLSDLMTQLRVRSVDEYGLRLHQAMADIA
                     PGGISDPQVVLLSPGIFNSAYFEHVFLAREMGVPLVEGQDLIVEDGAVWMRTTAGLAP
                     VHTIYRRLNDDFLDPDAFRPDSQLGVRGLIDAWRRGAVSIANAVGTGVADDKAVYAYM
                     PRIIRYYLGEDAILQNVDTRSCAEWEGLAYTLAHLPELVVKPVAESGGYGLLIGPHAS
                     KAELEEFRQKLAANPANYISQPMIKLSVSPTLCDDGVKPRHIDLRPFAITGRDTWVLP
                     GGLTRVALRAGSLVVNSSQGGGSKDTWVLE"
BASE COUNT          770 a         1376 c         1537 g          766 t
ORIGIN      
        1 cgaagatgcc aatggagcgg tccgttccct tcacgatgaa gcggccactg gagaactcca
       61 tatcggcgac ggccgcttcc aggatgtggc cggcgatacg caggcccttc gcgatgatct
      121 cgttcgtcgc gccaagcatc acgatgctgc caagccggag cgcgcgcccc gaatgcgagc
      181 caccaccgac ggagacgcgc gcggtgtcgc cctgcaccag caccacgctt tccattggca
      241 cgccaagcca ctcgccgacc agctgcgcga agctggtctc gtggccctgg ccgctggaga
      301 ccgtgccgat gacgagctcg accacaccgg acggcagcac ggttatctcg gcgcgctcgc
      361 gcggcacgcc ggtggcggta tcgacgtagt tggcgatacc gatgccgcgc agcttgcctc
      421 gtgcgcgcgc tgcgtcgcgg cgtgaggaga agccttgcca gtcgccgagc gtgagcacgg
      481 tctccatcac cttgtgatag gcgccactgt cgtattccag gccgaacgga ttgcggtagg
      541 gcatcgccga ctcaggcacc aggttgcggc ggcgcagctc cacgcggtcg atgccgcatt
      601 ggcgcgcggc gatgtcaatc aggcgttcga cgacgaacat cgcctcggga cggccggagc
      661 tgcgataagg gcgtgtcggc gcagtgttgg tcagagtggc gcaggcgcgg aaatgcaccg
      721 tggggacatg gtagatgctg ctcatgatct ccacgccctt gtgcaagggg ccgaaggaga
      781 ccggatacgc gccgctgtcc acgatgttgg tgccgcgcat cgcgaggaat ttgccgttag
      841 catcgagcgc gagttccgca tctgcggtga ggtcgcgggc ctgatagtcg cacaggaacg
      901 cctcgctgcg ctcgcaggtc catttcacgg gccggtggac gcgcttcgca gcccaggcga
      961 cgagagcaaa ctcgggattg gaggcgccgc gggtgccgaa attgccaccg acgtcgtgca
     1021 tcaccatgcg gacgttgtcg tcggggactc cgaggacgac agccatgtcg tggcgggggc
     1081 gcacggcgcc gcccgcaccg gcatggagtg tatagctgtt tgtggtggcg tcatattcgc
     1141 cagtggcggc gcgcggctcc atggtgacgc cggcgatgcg ctgcacgcgg gtggcgagac
     1201 ggacaacgtg tgcggcggag gcgaatgcgg cgtcggtggc ggcggcgtcg ccgatctggg
     1261 cgtcaagaca aaaattggac cgggcgtcgg accaggtgag cggtgcgttc ggagcgatgg
     1321 cggcgcggga agacacgacg gcggcaagat cggcgtaact gacgatcacc tgctcagcgg
     1381 cgtccttggc ggcggtgcgt gacgtggcaa cgaccatggc gacaatgtca ccgatgtgac
     1441 gcgcctcgtc ggtagccatc gcatagtgcg gcggaatgaa aggcggcgag ccgtctgaat
     1501 tgtcgagcgc aatgtcagca ggatggccgg tgcgcacggc atgagggatc ggcttcaggc
     1561 cgtctgccag catgtcccga ccggtcagga cggcgagaac gccgggggca gtggctgcca
     1621 cggcggtgtc gatcgagcgg atgaccgcgt gagcgtgcgt ggagcgcacc atgaccgcat
     1681 gggcctgtcc cggcacattg atgtcgtcgc tgtagcgccc gcggccggtg agaaggcgca
     1741 ggtcctcacg gcgccggaca ggctggccga tccctgtgcc ggtagcattg tgcagcattg
     1801 gacctccctg cggcgcgtgt tcgggccgcg ttgagggagg gtggccgatg gttggatgcg
     1861 cggcaagttg tgtccgtgtc attgcgaccc cggccttcgc cggggtatca atgacaggtt
     1921 cagtcaggct tggctctggg tctgctcctg ttccaccagg accggcgcgc cgcagagccg
     1981 ggtcatgata tcggtgtgca gcgcgccaag ctctcgctgc atccagtcca ggaactgcga
     2041 caggccgcgg cgcaggatct cctccaccgt ctgcgcggcc agcatcgact ggagctcgtc
     2101 cagccgcgcc agcgcaggcg tgcagccgcg caggtggtag cggctgcgca gcgtcgtcag
     2161 atgccaatcg agctgcgcca ggctcaagcc agcagagcgg ggaaaggccg tgttcaacag
     2221 caggaaaccg gcgacttcgg gcggacgata tccgtgtgga tattcccgtc gatacgcatg
     2281 gtaaccggcg gcagcgcgca gcaactcgtt ccactcgccg gcgtcgatcg ccgcatctgc
     2341 cgctttgggc atcagcgcgt tgaacttcgt atcaatcagc cgggtgatct ggtccgcgcg
     2401 ttcgatgtgg cggcccatca tgtagaagtg ccagcactgg tcgcgataga atgtgccctc
     2461 ggtgatgccg gtgtgagact gcacgccttc cttcagcatc gcgcagacgg cagagacttc
     2521 ctcgggcgag atgtccgcct cggtcagcgc acgtatctgg ccgtggaaca cgttgatctg
     2581 cagccacatc tcggtggaga tcagcgcgcg caaggtgcgg gcgttctcgc gtgcatagcg
     2641 gatgccggac tgcacggatg tgggattgtc gccgtccagc agatagaacc gcgcgacgct
     2701 ggtggcgtcg gcgacctggt tggtggcgaa aaaagtgccc gtgtcgccgt tgatccgcag
     2761 cagcgacagc caattgcgat ggctatcgtc gtcacgcgcg aaggtcttgg tgacatccag
     2821 cagacgcgca agattctcga tgcgctccat gtagcgcgcg agccagagcg cgctgccggc
     2881 atggcgtgcg atcaggtgct tgctgacgaa cacggcctcg ctcattccag cacccaggta
     2941 tctttcgagc cacccccctg tgacgagttc accaccagcg agccggcacg gagtgccacg
     3001 cgcgtcaggc cgcccggcag gacccaggtg tcgcgcccgg tgatcgcaaa ggggcgcaga
     3061 tcgatgtggc gcggtttcac gccgtcatcg cacagcgtcg gactgaccga gagcttgatc
     3121 atcggctggc tgatgtaatt ggccggattg gccgcgagct tctgccggaa ctcctccagt
     3181 tcagccttgc tggcgtgcgg gccgatcagc agaccgtagc cgccggattc tgcgaccggc
     3241 ttcacaacca gctcaggtaa atgcgcgagt gtatacgcca gaccctccca ctcggcacag
     3301 cttcgtgtat cgacgttctg caggatggca tcctcgccca gatagtatcg gatgatgcgc
     3361 ggcatgtagg cgtatacggc cttgtcgtcg gcaacaccgg tgccgactgc gttggcgatg
     3421 gaaacggctc cgcggcgcca cgcatcgatc aggccgcgta cgcccaactg gctgtccggg
     3481 cggaaggcgt cggggtccag gaaatcgtcg ttgaggcggc gatagatcgt gtggaccggc
     3541 gcgagtccgg cggttgtgcg catccagacc gcgccgtcct cgacgatcag gtcctgacct
     3601 tcgaccagag gcacgcccat ctcgcgcgcc aggaaaacat gctcgaaata ggcggagttg
     3661 aagatgccgg gcgagagcag caccacctgc ggatcgctga tccctccagg tgcgatgtcg
     3721 gccatcgcct ggtgcaggcg cagcccgtac tcgtcgacgc tgcgcacgcg cagctgggtc
     3781 atcaggtcgg acagcacgcg cagcgtcatg tggcggtttt cgaccacata ggcgacaccg
     3841 gagggcgtgc gtgcgttgtc ctccagcacc agatagcggc cggaatcgtc tcggatgatg
     3901 tcgatgccgc agacatgcac atacgtcttg tgcggcgggc tgaacccaac catcgcctgg
     3961 cagtacgcgg cgttgccgag caccaactcg cgcgggacca cgccatcctt caggattttt
     4021 ccgtcgtgat agatatcgtg caggaacagg ttcagcgcac gcacgcgttg ggcgacgcct
     4081 gattccaggg tgtgccattc ctctgctgtg atgacgcggg ggatcaggtc gaacggcagg
     4141 atgcgatcga tcgcggtcgc gtcggagtag acagtgaacg tgatgccgcg ttccagcagg
     4201 tcgcgctcgg catcagcgga gcggcggcgc agtgagtcga tcgggagctc gccgagacgc
     4261 tcacgcagga gcgtcagcgc gggatgcggt ggctggccag gacggaacat ttcgcagaac
     4321 aacgcgcctg gatcgtagcc cgcgagcacg ccgtcctgtt gcattcaccg cccccgggcc
     4381 agacttttcc cacgcgatat cctgcacatt ctgtgccggg attgctacga cgccgtgcca
     4441 tcgggcagt
//
LOCUS       DATGEM010000533         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1286770, whole genome shotgun sequence.
ACCESSION   DATGEM010000533 DATGEM010000000
VERSION     DATGEM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286770"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1045)
                     /gene="ltrA"
                     /locus_tag="VKQ27_16245"
     CDS             complement(287..1045)
                     /gene="ltrA"
                     /locus_tag="VKQ27_16245"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HLY90534.1"
                     /translation="MKQVRANKGAAGVDGLDIDQTSRMLRTEWPAIRERLLEGTYRPQ
                     PVRRVMIPKPGGGERELGIPTVLDRLIQQALLQVLQPIPDPTFSTHSYGFRPGRSAHD
                     AVLSAQSFVQSGRRVVVDVDLEKFFDRVDHDILIDRLRKKVPDPGVIRLVRAFLNADI
                     VDGDAVIKRTRGTPQGGPLCPLLANVLLDEVDKELERRGHAFCRYADDCNVYVRSKRA
                     RERVMALLRRLYGRLHLTVNETKSAAASWAMPPG"
     gene            complement(1768..2511)
                     /locus_tag="VKQ27_16250"
     CDS             complement(1768..2511)
                     /locus_tag="VKQ27_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLY90535.1"
                     /translation="MANWSDPRTTAVPRAATGAREAAFDAGLRSYMLSVYNYMASGVL
                     LTGIVAMLFARGGADSMAAQIFMQGGLLRFLVMLAPLGVVMWMSFGINRISEGTAKAL
                     FWVYAALMGLSLSSILLVYTGGSVAQAFFATAASFAALSLFGYTTKKSLSGMGTFLIM
                     GVFGLMIAMLINMFVHSSAMDLLISAVGVLLFAGLTAYDTQRIKSSYFAVRGTAYAGK
                     SAVMGALTLYLDFINLFLFLLRFMGNQRN"
     gene            2614..>3093
                     /gene="thpR"
                     /locus_tag="VKQ27_16255"
     CDS             2614..>3093
                     /gene="thpR"
                     /locus_tag="VKQ27_16255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HLY90536.1"
                     /translation="MAHHRLFVAIRPAAPVRARLLGLAIGIPGARWQSDDQLHCTLRF
                     IGEVDHHQAEDIAALLGHVRHPAMTLALGDVGTFERGHRIDTLWIGVQPREPLKALHD
                     KIDRLLREAGIASDTRAFLPHITLARFGRHALPPRDLASRFAPPPAEPFAMAAFVLCE
                     "
BASE COUNT          558 a         1076 c          898 g          561 t
ORIGIN      
        1 accgcctgac ttggcgatcc gtgatccaat ttcccggcat cggcatgttc ccctgcgatc
       61 gcaggggaac atgccgatca actcttcacg aaccggccat catggttgtc attaggcggc
      121 cttcgtaact gacggcgtgc attccagccc agaacaaaag accgcagacc cgccgtagcc
      181 gcagccagcg agcgcccggt cacccggcgc gttagcaggc ggatacggtc cttgaacgcc
      241 ttgatcgcct tgtcggcgac ccgtcgtttg acgacgcgcc ccggagctac ccaggaggca
      301 tagcccagga agcggcggca ctcttggtct cgttcaccgt cagatgaagt cgaccataga
      361 gacgccgtag cagcgccatg accctctctc gcgcccgttt ggaccgcaca tacacattgc
      421 agtcgtcggc gtagcggcag aaggcatgac cccgccgttc aagctccttg tccacttcgt
      481 cgagcagcac attggccagt agcggacaca aaggaccgcc ttgcggcgtc cccctggtcc
      541 gtttgatgac cgcatcccca tcgacaatgt ccgcgttcag aaaggcgcgg accagacgaa
      601 tgacgccggg gtcggggact ttcttgcgca ggcgatcgat caggatgtcg tgatccaccc
      661 ggtcgaagaa tttctccagg tccacatcca ccacgacccg tcgcccggac tgcacgaacg
      721 attgtgccga caagacggcg tcgtgcgcgc tcctgcccgg gcgaaagccg tagctgtggg
      781 tgctgaaggt gggatcaggg atcggttgca gaacttgcaa gagcgcctgt tggatcaggc
      841 gatccagcac tgtagggatg ccgagctcac gctcgccgcc ccccggcttc gggatcatca
      901 cacgacgcac cggttgtggc cggtacgtcc cctctaacaa gcgttcgcgg atggcgggcc
      961 actcggtgcg cagcatccga gacgtctggt ctatatccag gccgtctacg cctgctgcgc
     1021 ccttgttggc gcgcacctgc ttcattgctc gcaggaggtt ctctcgcgcc agaactgctc
     1081 ctagagcgaa atccgctccg atcgcatcgg atcggctact ctaggtcgtt ggtttgacgc
     1141 gatttccgag tcagcggatg attccatccg cttcgaaatc gctctagcag ccctggcccc
     1201 gtgtcgttcc tggtccgcag cgggagcgtg gcttcgtcga gacaggagcc atgacgggct
     1261 tcacccgcct gcctctcctg actcccgggc gtgcccgaca tctgacgcgt ggccgtactc
     1321 atccttgggg gaacttcaca ctctctcgtt cggcccttcg ctctcggttt cccgtcagct
     1381 actacggctt cggctgactt ctcgctccgg ctcgacaccg tcgccctttc aggcacaagg
     1441 cgagatctcc ccaggtaaga acgcattcct tcactgcacg gccgccggat ttacgccgct
     1501 cccccttgat cacgagagct tcgcggttcc atgcccgctc gccttggtgg gcagcgcctc
     1561 atatccagtt cttgtccatc ggcccgcagc ttcgctccac gcttcctccc cacggtcggt
     1621 cgcccttccg cagttgcgct tcacttcgct cgccgtgatc agctcacgat gggactctca
     1681 cccataagaa cgcgcccatg ctcggcgcac aaagcaaagg gcccggcgga tcgctccgtc
     1741 gggccctccg gtctcggctt cgcctgatca gttcctctga ttgcccatga agcgcagcag
     1801 gaacaggaac aggttgatga agtcgagata gagcgtgagc gcgcccatca ccgcagactt
     1861 gccggcatag gccgtgccac gcaccgcgaa atagctgctc ttgatccgct gcgtgtcata
     1921 ggcggtgagg cccgcgaaca gcagcacgcc gaccgccgag atcagcagat ccatcgccga
     1981 ggagtgcacg aacatgttga tcagcatcgc gatcatcagc ccgaacacgc ccatgatcag
     2041 gaatgtgccc atgcccgaca ggctcttctt ggtcgtgtag ccgaacaggc tcagcgccgc
     2101 gaagctcgcc gccgtggcga agaaggcctg cgccaccgaa ccaccggtat agaccagcag
     2161 aatcgacgag agcgaaaggc ccatcagcgc cgcatagacc cagaacagcg ccttggcggt
     2221 gccttccgaa atccgattga tgccgaagct catccacatc accacgccga gcggcgccag
     2281 catcacgagg aaacgcagca agccgccctg catgaagatc tgcgcggcca tcgagtccgc
     2341 gccgccgcgc gcgaacagca tcgcgacgat gcccgtcagc agcacgcccg aggccatgta
     2401 attgtaaacg gacagcatgt aggaccgaag tccagcgtcg aacgccgcct cacgggcgcc
     2461 cgtcgcagcg cgaggcaccg cggtcgtccg ggggtcagac cagttcgcca tcgaagatac
     2521 tctccttacc gtcgacgatg tgcgtcgaca tcgccaatat cgtggtttcc gaatgtcctt
     2581 tcaagcgaaa ccggatttcg cgggagaacc cttatggcgc atcatcgcct gttcgtggcg
     2641 atccggccgg cggcgccggt tcgcgcccga ctgctgggcc tcgcgatcgg catccccggc
     2701 gcccgctggc agagtgacga ccagctccat tgcacgctcc gcttcatcgg cgaagtcgat
     2761 caccaccagg ccgaggacat cgccgctctg ctggggcatg tccggcatcc ggcgatgacg
     2821 ctggcgctcg gcgacgttgg cacgttcgaa cgcggccacc ggatcgatac actatggatc
     2881 ggcgtgcagc cccgcgagcc gctgaaggcg ctgcacgaca agatcgatcg cctgctgcgc
     2941 gaggctggta tcgcctccga tacgcgcgcc ttcctgccgc acatcacgct ggcccgcttc
     3001 ggccggcacg ccctgccgcc ccgcgacctc gccagccggt tcgctccgcc gcctgcagag
     3061 ccctttgcca tggcggcgtt cgtcctgtgc gag
//
LOCUS       DATGEM010000534        12748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_859627, whole genome shotgun sequence.
ACCESSION   DATGEM010000534 DATGEM010000000
VERSION     DATGEM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859627"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VKQ27_16260"
     CDS             complement(<1..1112)
                     /locus_tag="VKQ27_16260"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLY90537.1"
                     /translation="MKYLGIVEALEADVRSGRVGPGDRLPSQRAIAEALRVDLTTVTR
                     AFNEARRRGLIDAHAGRGTFVRDDLDTDRAPGRFESAPRLDLSMNIPPQPAGAHLATR
                     IPSGIAALLSSPRGMLHLHYQESTGTDADRAAAAVWLGRRMETVPANRILVTGGAQSA
                     LFAICESELRAGDRVAAGALTYPGIKAIAAQKGFSLVPLAMDAEGIVPDAFEQACRGT
                     PPKALYVIPSIDNPTTATLPEDRRRQLVAIARRYGVMIIEDDPYTPLLVRPVPAIADL
                     AGDITWHVATLSKCATPALRIAYVVAPGSAQALRLAAVLRATNMMASPLMAALASQWI
                     ASDLLDAIAATIRAENGERQKLAAAILAGQDVSVDP"
     gene            complement(1239..1610)
                     /gene="cyoD"
                     /locus_tag="VKQ27_16265"
     CDS             complement(1239..1610)
                     /gene="cyoD"
                     /locus_tag="VKQ27_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HLY90538.1"
                     /translation="MSDHMMHDGGHDAAHGSLTSYAVGFLLSLLLTAGSFGAVMSGAV
                     PHAMILPAIVVLAVTQLLVQLILFLHLGSAPDQRSNTVIFLLTGMLIVIIVAGSLWVM
                     HNANVNMMPMQMSVERALAKN"
     gene            complement(1610..2224)
                     /gene="cyoC"
                     /locus_tag="VKQ27_16270"
     CDS             complement(1610..2224)
                     /gene="cyoC"
                     /locus_tag="VKQ27_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HLY90539.1"
                     /translation="MNSPVSTLHGGHGGHEHGHDAGANTVLGFWIYLMSDCLIFAILF
                     ATFGVLSTSYAGGPSGRELFDLPYVAGETLLLLVSSFTFGVAMLSMQARRTDNVIILL
                     LVTFLLGTGFIWMEVHEFAHLIAEGAGPDRSAFLSAYFVLVGTHGLHVTVGLLWLAVM
                     MHQIARYGLDGIVRRRLSCLSLFWHFLDLVWICVFTFVYLRGVL"
     gene            complement(2226..4202)
                     /gene="cyoB"
                     /locus_tag="VKQ27_16275"
     CDS             complement(2226..4202)
                     /gene="cyoB"
                     /locus_tag="VKQ27_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HLY90540.1"
                     /translation="MLGKLTLDAIPWHEPIIMGTGAVVALGGAAMLAALTWFRLWGPL
                     WKNWITSVDHKKIGVMYIVLALVMLLRGFADAIMMRGQQALAANDGAGFLPPHHYDQI
                     FTAHGVIMIFFVATPFVLGLMNVIVPLQIGARDVAYPFVNSLSFWLSAFGAVLVMASM
                     WIGDFAATGWVAYPPLSELGYSPTVGMDYYIWSLQISGLGTTLTGINFIVTILRMRAP
                     GMGLMKMPVFTWTALITNILIVAVFPVLTATLALLTADRYLGMHFFTNELGGNAMMYI
                     NLIWVWGHPEVYILILPAFGAFSEIIATFSGKPLFGYKSMVYATSSIGVLSFFVWLHH
                     FFTMGSGANVNAFFGIMTSIISIPTGVKLFNWLFTMFRGRIRYHSSVLWTVGFMVTFA
                     VGGMTGVLLAVPGADFVLHNSLFLVAHFHNVIIGGVIFGCLAGMSFWFPKVFGFTLNE
                     FWGKVAFWCWLVGYWLAFTPLYILGFKGMTRRMNHYDHPDWHPYLIVALVGAVIIGMG
                     ILAIGIQLYVSIRDRDQNRDLTGDPWDARSLEWATASPAPFYNFAVLPEITSLEQHWD
                     DKEHGRHFVQPTRYEDIHMPKNTGTGFIISAFGFVMCFALVWHMWAIAAAGLLGVIGT
                     FLFRAYDRDVDYWVPAAEVQAIERRRTDMIRKAA"
     gene            complement(4208..5170)
                     /gene="cyoA"
                     /locus_tag="VKQ27_16280"
     CDS             complement(4208..5170)
                     /gene="cyoA"
                     /locus_tag="VKQ27_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HLY90541.1"
                     /translation="MQVMAGARRTSAAFRALPGLRPIGRALARSVGALGLLSLGGCNI
                     ELLNSQGSIGQQEKMLILIALGLMLLVVIPVMALTLFFAWRYRASNTKANYQPKWAHS
                     TAIEWVVWTIPCLIVAFLAVLIWGTTHALDPYKPLESKAAPVKVEVVALDWKWLFIYP
                     DYGVASVNRLEIPAGRPIDFKLTSQSMMNSFFIPELGSQIYAMAGMQTQLHLIADRPG
                     TYRGLSSAFSGPGFSDMHFDTVATDERGFAQWVSQAKATPLTLDKASYTMLAKPSEKN
                     PVRIYARVSPGLFDGIVNQYMSGPAPAGAPMKAMAMRHDAMAAE"
     gene            complement(5546..7864)
                     /locus_tag="VKQ27_16285"
     CDS             complement(5546..7864)
                     /locus_tag="VKQ27_16285"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HLY90542.1"
                     /translation="MSLVRRFALASALIAATPALASPAYDAVDPMIGTGGEGHTFPGA
                     AAPFGMVQLSPDTDTTCAIRACYGHAAGYRYADPTIQGFSHTHFSGAGHSDLGDFLVM
                     PVSGDAVVTEPGDTAKPGSGYRSRFDHATEVAQPGYYAVTLADPGVRAEMTVGTRIGV
                     HRYSFPKGKAAHLVLDLRTSLYNYPGKILWSSIRLRPDGTITGARETRGWAPDRKLHF
                     AMRFSAPLINHAFLNTEDNIPYKGFQAPGRGSDALAEAMGKALVARLDFGTLDTPLEV
                     KVAISSVDEAGAIANLDAEPGDFDAIRTRTKGGWEQALNAVDLDAPAPMKKSLYTALY
                     HSLLAPSVASDVDGRYRGPDNQVHTADGFTFRSTFSLWDTFRAEHPLLTIIEPKQTNV
                     DFIRSLIESQKESPDGILPIWQFQGRETWTMIGYHAVPIIADAYLKGIDGFDPREALH
                     AMVASATYAPYGGLGDYMKLGYVPIDKEPEAASKTVEYAFDDWTIAQFAKKIGRQDIA
                     ATFDKRAQNWRNGFDAKTGWVRARLSNGQFRAPFDPVSIKYGSDYTEGNAWQYSWFMP
                     QDEGGMIRLLGGDAKTVAKLDAMFAYDNSKLDYSSSEDIAGLIGQYIHGNEPSHHVAY
                     LYSYAGAPWRTQERLKQIVDSQYKPTPEGLAGNDDLGQMSAWLVFTGFGFYPVTPGSL
                     EYVIGRPFVEHATLTLPNGKRFRIVTEGLGDEHPYVGKVLLNGQPLEKGFITHAQIEA
                     GGELRFVMSATPNKTWATKAADRPYSMSGYAR"
     gene            8033..8311
                     /locus_tag="VKQ27_16290"
     CDS             8033..8311
                     /locus_tag="VKQ27_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90543.1"
                     /translation="MKRVLILACVTSLAAGGIATEASAQSAHHILRGAGIGAAGGVIA
                     GALIPGLGIGTGALVGAAGGALYNTLDKGHHHYYHHSGYRYRAHHSYR"
     gene            8551..9210
                     /locus_tag="VKQ27_16295"
     CDS             8551..9210
                     /locus_tag="VKQ27_16295"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY90544.1"
                     /translation="MPRTSDAGQPACLLIPGRDHPRRNDWQSAWIRERDDCIGIDFGS
                     LHDPIRSVWLSRIDQAVGMARSRVVLVEHDLGCLAVAWWAALLDKRAARRVIGALLVA
                     PPDPDAPDSDDRVRRFGPLPEAMLPFPAILVASRDDPNATIERSRAMAAEWVTDFFDA
                     GSLGHLDARSHLNAWPDGQRLLDILIDGGPGLSRYTYRRPPPPRPASRPVHRMTPSSQ
                     P"
     gene            9273..9437
                     /locus_tag="VKQ27_16300"
     CDS             9273..9437
                     /locus_tag="VKQ27_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HLY90545.1"
                     /translation="MATHAAYMEGFRAATLRHSREKCPYPPDTREQAEWVAGWEAGDD
                     HDEVGECSSD"
     gene            complement(9489..9962)
                     /locus_tag="VKQ27_16305"
     CDS             complement(9489..9962)
                     /locus_tag="VKQ27_16305"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLY90546.1"
                     /translation="MTPRIGPTVVREAVAGLSAEGWEEARQGSGVFVKVAPRVVQVTA
                     KELPNLEDVLALLELRIAVETEMAGLAAERRADVAAIEAWMEEFCAAIDEQADGIDAG
                     AALYAAIARATRNQHFVRFIAFHGTILLPCRAVPALFAFASLTSFPVIGLSLGSI"
     gene            10530..11096
                     /locus_tag="VKQ27_16310"
     CDS             10530..11096
                     /locus_tag="VKQ27_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90547.1"
                     /translation="MNDGLAHGRYFFMDVARDGIALYESDSSELHTPKPKTPAQALAM
                     AKEYFEEWFPSALVYLGTARDLVTQGRNEEPAFILHQSAERLYHCVLLVVTFYTPHVH
                     NLGFLRTQAERIENLGQVVHAVCSDRIAKLEQDARQGSPDLPAHQRPSVPTHHGSPAA
                     ILAAISASFTGTSARASSTLHPCATRVR"
     gene            complement(10988..11344)
                     /locus_tag="VKQ27_16315"
     CDS             complement(10988..11344)
                     /locus_tag="VKQ27_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90548.1"
                     /translation="MRLIEKVRMMERMGAAEGRCGVGKWVSGWRRLRRLQRAVDTMPE
                     VLYAVFGAVRYEDLGYGAIAARLGVGVDEVERRFAWSLYYLTRVAHGWSVEEARAEVP
                     VNEAEIAARMAAGEPW"
BASE COUNT         2523 a         4152 c         3960 g         2113 t
ORIGIN      
        1 tgcggatcga cagacacgtc ctgtccagcg aggatcgccg ctgccagttt ctgccgctcg
       61 ccattctcgg cgcggatcgt cgccgcgatg gcgtcgagca gatcgctggc aatccactgg
      121 gaagccagag ccgccatcag cggagacgcc atcatgttgg tcgcgcgcag cacggctgcc
      181 agccggagcg cctgcgcgct gcccggggca accacatagg caatccgaag cgcaggcgtg
      241 gcgcatttgg acaatgtcgc gacatgccag gtgatgtcac cggccagatc cgcgatcgcc
      301 ggaaccgggc gcaccagcaa gggcgtgtag ggatcgtcct cgataatcat cacgccatag
      361 cggcgcgcga tggcgacgag ctggcgccgt cggtcctcgg gcagggtcgc cgtcgtcgga
      421 ttgtcaatcg agggaatgac gtagagcgcc ttgggcggcg tgccgcggca ggcctgctcg
      481 aacgcatcgg gcacgatccc ctccgcatcc atcgcgagcg ggaccagcga gaaccccttc
      541 tgcgccgcga tcgccttgat tcccggatag gtcagcgccc cggcggcgac gcgatctccc
      601 gcccgcagct cgctttcgca gatcgcgaac agcgcgctct gcgcgccgcc cgtcaccagg
      661 atccggttgg ccggcaccgt ctccatccga cgccccagcc agaccgccgc cgccgcgcga
      721 tcggcatcgg taccggtgct ctcctgataa tgaagatgga gcatgccgcg cgggctcgac
      781 agcaacgccg cgatcccgct gggaatgcgc gtcgcaaggt gcgcgccagc tggctgcggg
      841 ggaatgttca tgctcagatc gagacggggc gcgctttcga agcgacccgg cgcacgatcc
      901 gtatcgagat catcgcgaac gaaagtaccg cgcccggcat gggcatcgat cagaccgcgg
      961 cggcgcgctt cgttgaacgc gcgcgtcacc gtagtcagat cgacacgcag cgcctcagcg
     1021 atagcgcgct gcgatggcag gcgatcgccc ggcccgaccc ggcccgagcg cacgtccgcc
     1081 tccagcgcct cgacgatccc cagatatttc atccgggcat tctcgacgag tcttgggcgc
     1141 caggaagaca ttgtccgcta ttccgtaggg cgtttcagca tacagcttcg gtaccacgcc
     1201 ccccagtggc acggaagccg ttcgttctcc gccggcgctc agttcttggc tagcgcgcgc
     1261 tccaccgaca tctgcatcgg catcatgttc acattggcat tgtgcatcac ccagagcgac
     1321 cccgcgacga tgatcacgat cagcatgccg gtgagcagga agatcaccgt attgctgcgc
     1381 tgatcgggcg cgctgccaag gtgcaggaag aggatgagct ggacgagcag ctgcgtcacc
     1441 gccagcacca cgatcgccgg caggatcatc gcgtgcggca ccgcgccgct catcaccgcg
     1501 ccgaaggaac cggcggtcag cagcagcgag agcaggaagc cgaccgcata ggatgtcagg
     1561 ctgccatgtg cggcgtcatg gccgccatcg tgcatcatat ggtcgctcat cacagcaccc
     1621 cacgcagata gacaaaggtg aagacgcaga tccagaccag atcgaggaag tgccagaata
     1681 ggctgaggca ggagagccgg cggcggacga tgccgtcgag gccgtagcgc gcgatctggt
     1741 gcatcatcac cgccagccag agcaggccca cggtgacgtg cagcccgtgc gtcccgacca
     1801 gcacgaaata ggccgagagg aaggcgctgc gatcagggcc ggcgccttcc gcgatcaggt
     1861 gcgcgaactc atgcacctcc atccagatga agccggtgcc gagcaggaag gtgacgagca
     1921 gcaatatgat gacattgtcc gtccgccgcg cctgcatgct gagcatcgcc acgccgaagg
     1981 tgaagctgct caccagcagc aggagtgtct cgcctgccac atagggcagg tcgaacagct
     2041 cgcggccact ggggccgccg gcatagctcg tcgagagcac gccgaaggtg gcgaacagga
     2101 tcgcgaagat gaggcaatcg ctcatcaggt agatccagaa gccgaggacg gtgttcgccc
     2161 ccgcatcatg accgtgctcg tggccgccat ggcccccgtg cagggtggaa accgggctgt
     2221 tcatgtcagg cagcctttcg gatcatgtcg gtgcgacggc gctcgatcgc ctggacctcg
     2281 gcggcgggca cccaatagtc gacgtcgcgg tcataggcgc ggaacaggaa ggtgccgatc
     2341 acgccgagca acccggcggc agcgatcgcc cacatgtgcc acaccagcgc gaagcacatc
     2401 acgaagccaa aggccgagat gatgaagccc gtgcccgtgt tcttgggcat gtggatatcc
     2461 tcgtagcggg tcggctgaac gaagtgtcgg ccatgttcct tgtcgtccca atgttgctcg
     2521 agcgaggtga tctccggcag aaccgcgaag ttgtagaagg gcgcaggcga ggcggtcgcc
     2581 cattccaggc tgcgcgcatc ccacggatcg ccggtgagat cccggttctg atcgcgatcg
     2641 cggatgctga catagagctg gatgccgatc gccagaatgc ccatgccgat gatcacggca
     2701 cccaccagcg caacgatcag atagggatgc caatcgggat gatcataatg gttcatccgc
     2761 cgcgtcatgc ccttgaagcc aaggatgtag agcggcgtga aggcgagcca gtagccgacc
     2821 agccagcacc agaaggctac cttcccccag aactcgttga gggtgaagcc gaacaccttg
     2881 gggaaccaga agctcatgcc ggcgaggcat ccgaagatca cgccgccgat gatgacgttg
     2941 tggaagtgcg ccaccaagaa cagcgagttg tgcagcacga agtccgcacc cggcaccgcg
     3001 agcaggacgc ccgtcatgcc accgacggcg aaggtcacca tgaagccgac cgtccacagc
     3061 accgacgaat gatagcggat gcggccgcgg aacatcgtga acagccagtt gaacagcttc
     3121 acgccggtcg ggatcgagat gatgctcgtc atgatgccga agaaggcatt tacgttggcg
     3181 cccgaaccca tcgtgaagaa gtggtgcagc cacacgaaga agctgagcac gccgatcgac
     3241 gaggtggcgt agaccatcga cttgtagcca aacaacggct tgcccgagaa ggtcgcgata
     3301 atctccgaga aggcaccgaa agccggcaga atgaggatgt agacctcggg atgcccccag
     3361 acccagatca ggttgatgta catcatcgca ttcccaccaa gctcgttggt gaagaagtgc
     3421 atgccgaggt aacggtcagc ggtgagcagc gcgagcgtag cggtcagcac cgggaagacc
     3481 gcgacgatca ggatgttggt gatcagcgcc gtccaggtga acaccggcat cttcatcaga
     3541 cccatgcccg gcgcgcgcat gcgcaggatg gtgacaatga agttgatgcc tgtcagcgtc
     3601 gtaccgaggc cggatatctg gagtgaccag atataataat ccatgccgac ggttggactg
     3661 tatccaagct ccgagagcgg cggataggcc acccagccgg tcgccgcgaa gtcaccgatc
     3721 cacatcgacg ccatcaccag caccgcgcca aacgccgaca gccagaagct cagcgagttg
     3781 acgaaggggt acgcaacgtc acgcgcgccg atctgcagtg gcacgatgac gttcatcagg
     3841 ccgagcacga aaggcgtggc cacgaagaag atcatgatca cgccgtgcgc ggtgaagatc
     3901 tggtcgtagt gatgcggcgg caggaaaccg gcgccatcat tggcagccaa cgcctgctgc
     3961 ccgcgcatca tgatcgcgtc ggcaaagccg cgtagaagca tcaccagcgc gaggacgatg
     4021 tacatcacgc cgatcttctt gtgatcgacc gacgtgatcc agttcttcca cagcgggccc
     4081 cagaggcgga accaggtgag cgcggcgagc atcgccgcac caccgagagc aaccaccgcg
     4141 ccggtgccca tgatgatggg ctcgtgccac gggatcgcat cgagcgtcag ttttccgagc
     4201 atgttgttca ctccgcagcc atggcgtcat ggcgcatggc catggccttc atcggcgcgc
     4261 ccgcgggggc ggggccggac atgtactgat tgacgattcc gtcgaacaaa ccgggcgaaa
     4321 cgcgcgcata gatgcgcacc ggattcttct cgctgggctt ggcgagcatg gtgtagctcg
     4381 ccttgtcgag cgtcagcgga gtggccttgg cctggctcac ccattgcgcg aagccgcgct
     4441 catcggtggc gaccgtgtcg aaatgcatgt ccgagaagcc ggggccgctg aacgccgagg
     4501 acagcccgcg ataggtgccg ggacgatcgg caatcaggtg gagctgcgtc tgcatccccg
     4561 ccatcgcgta gatctgactg ccaagctcgg gaatgaaaaa ggagttcatc atcgattgtg
     4621 aggtgagctt gaagtcgatc ggccggcccg ccgggatctc cagtcggttg accgaggcga
     4681 cgccgtaatc cgggtagatg aacagccact tccaatcgag cgcgaccacc tcgaccttca
     4741 ccggtgcggc cttcgattcc agcggcttgt agggatcgag cgcgtgtgtc gtgccccaga
     4801 tcagcaccgc cagaaacgcc acgatcaggc agggaatggt ccacaccacc cactcaatcg
     4861 cggtcgagtg cgcccatttg ggctgatagt tcgccttggt gttggacgca cgatagcgcc
     4921 acgcaaagaa cagcgtcagc gccatcaccg ggatcacgac gagcaacatc agccccagcg
     4981 cgatcaggat cagcatcttc tcctgctggc cgatcgagcc ctgcgagttg agcagctcga
     5041 tgttgcagcc gccgagcgac aggaggccca gcgcccccac cgatcgggca agagctcgcc
     5101 cgattggacg cagccctgga agggcccgga acgccgcgct cgttcgacga gccccggcca
     5161 tgacctgcat tgtcaccacg gcaatccttt ggatgcaccc gccccgccca cgcggcatcc
     5221 ctccagaaca cttcatgtcg atggaacggg aacgaagcga ttcaatttgt atggcaaata
     5281 tccatacagc catacatttg caatgcatcg catctcggcc tgaggcgcct aattttctat
     5341 ggaataagcg agcattatgc cgcaccgcgc ggagtcgaga ttgcggcaca gaccgcgcac
     5401 cgcgaatcag ccatacatat aatgatagaa gcctgacaat cggcagagca atcgtcacgc
     5461 caggcgcgca agtttccact gccggcgtgt cacatgccgg cggcgctccc ctgcccgatc
     5521 gggatcggcc ctatacgatc gcgccttagc gtgcgtagcc gctcatcgaa taggggcggt
     5581 cggccgcctt ggtcgcccac gtcttgttgg gcgtggcgga catgacgaaa cgcagctcgc
     5641 cacccgcttc gatctgggcg tgggtgatga aacccttctc cagcggctgg ccgttgagca
     5701 gcaccttgcc aacataggga tgctcatcgc ccagaccctc ggtgacgatg cggaagcgct
     5761 tgccgttggg cagggtgagc gtcgcatgct ccacgaacgg ccggccgatc acatattcga
     5821 ggctacccgg cgtcaccgga tagaagccga accccgtgaa cacgagccag gccgacatct
     5881 ggccgagatc gtcgttgccg gcgaggcctt cgggcgtcgg cttatactgg ctgtcgacaa
     5941 tctgtttcag ccgctcctgc gtccgccacg gggcgccggc gtagctgtag agataggcga
     6001 cgtggtggct gggctcgttg ccgtggatat actggccgat caggccggca atgtcctccg
     6061 agcttgaata atcgagcttg ctattgtcgt aagcgaacat ggcgtcgagc ttggcgacgg
     6121 ttttggcgtc accaccgagc aggcggatca tgccaccctc gtcctgcggc atgaaccagc
     6181 tgtactgcca agcattgccc tcggtgtaat cggacccgta cttgatcgac accggatcga
     6241 aaggcgcccg gaactggccg ttggaaagcc gtgcgcgcac ccagccggtc ttggcatcga
     6301 atccgttgcg ccagttctgc gcgcgcttgt cgaaggtcgc ggcgatgtcc tgccggccga
     6361 tcttcttggc gaattgggcg atggtccaat catcgaaggc gtactcgacc gtcttggagg
     6421 cggcttccgg ctccttgtcg atcggcacat agcccagctt catatagtca ccgagaccgc
     6481 cataaggcgc ataggtggcg ctggcgacca tcgcgtgcag cgcctcgcgc ggatcgaagc
     6541 cgtcgatccc cttgaggtag gcgtcggcga tgataggcac ggcgtgatag ccgatcatcg
     6601 tccacgtctc gcggccctgg aactgccaga tgggcaggat gccgtcgggg ctctccttct
     6661 ggctttcgat cagcgagcgg atgaagtcga cattggtctg cttgggctcg atgatggtga
     6721 gcagcgggtg ctcggcgcgg aacgtatccc atagcgaaaa ggtcgaacgg aaggtgaagc
     6781 cgtcggcggt atgcacctga ttgtccggcc cgcgataacg accgtcgaca tcgctcgcca
     6841 ccgaaggcgc cagcagcgag tggtagagcg ccgtgtacag gcttttcttc atcggtgccg
     6901 gggcatcgag atcaacggcg ttcagcgcct gctcccaccc gcccttcgtt ctggtgcgga
     6961 tcgcgtcgaa gtcgccgggc tcggcatcga gattggcgat cgcgcccgcc tcgtcgaccg
     7021 aggagattgc caccttcacc tcgagcggcg tgtccagcgt gccaaagtcg agccgcgcga
     7081 ccagcgcctt gcccattgcc tcggcaagcg cgtcgctgcc gcggccgggg gcctggaagc
     7141 ccttgtaggg gatattgtct tcggtgttga ggaaggcgtg gttaatcagc ggcgcggaga
     7201 agcgcatcgc gaaatggagc ttgcggtccg gcgcccagcc gcgcgtttcg cgcgcgccgg
     7261 tgatggtgcc gtccgggcgg agccggatcg aggaccagag gatcttgccc ggataattgt
     7321 agagcgacgt ccgcaagtcg aggacgagat gcgcagcctt gcccttgggg aagctgtagc
     7381 ggtggacgcc gatgcgtgtg cccacggtca tctctgcgcg cacgcccgga tcggcgagcg
     7441 tcacggcata atagccgggc tgcgccactt cggtcgcatg gtcgaagcgc gagcggtagc
     7501 cggagccggg cttggcggta tcgccgggct cggtcaccac ggcgtcgccc gacaccggca
     7561 tgacaaggaa gtcgcccaga tcgctatgcc ccgcacccga gaaatgggtg tggctgaagc
     7621 cctggatagt cgggtccgca tagcgatagc cggcggcatg gccatagcag gcgcggatgg
     7681 cgcaggtcgt atcggtgtcg ggcgagagct gcaccatgcc gaacggcgca gccgcaccgg
     7741 ggaaggtgtg gccttcgccc cccgtgccga tcatcggatc gactgcatca taggccggcg
     7801 aggccagagc cggcgtggcg gcgatcaggg cggaagcgag cgcgaagcga cggacgagcg
     7861 acatgaccac ctttagagca ggcactccat caaagcggaa gccggcacaa cgcggctccc
     7921 tcccgatgtc gttcagaatc ccctcaggtt gcgatggcta tctcggagca atcgaagcgg
     7981 acgggcgtta ccccccgatt gatcgcgtag gaagcgtagg aaagggagat atatgaaacg
     8041 ggttctgatt ttggcatgtg tcacctcgct cgcagctggc ggaatcgcca ccgaggcgag
     8101 cgcccagagc gcgcaccaca tcttgcgtgg cgcgggcatc ggcgccgcgg gcggcgtcat
     8161 tgcgggcgcc ctgatcccgg gcctggggat cggcaccggc gcactggtcg gcgcggccgg
     8221 cggcgcgctc tacaacacgc tcgataaggg ccatcaccac tattaccacc acagcggcta
     8281 tcgttatcgg gcgcaccact cctatcgctg aaccaccagc ggcgcgcaga ggtggccccc
     8341 gaacgacgca tgatcgtcga tcggcaccat cggcgcggat gattcgtccg cgccgccctc
     8401 ttcctgccgc ctgaccgggg agacgatccc gccgccttgc gccgaaacga ccgagggctg
     8461 cgacaacgtc gcttgcgctg aagtggccgt cagccatcat gatggctgac attcctgatc
     8521 gttgcgcgtc cgcctggcgg gagagcggtg atgccgagaa caagcgatgc cggtcagccg
     8581 gcttgcctgc tgatacccgg gcgggatcat ccacgtcgca acgactggca atcggcctgg
     8641 attcgcgagc gcgacgattg cattggcatc gacttcggca gcttgcacga tccgatccgc
     8701 agcgtctggc tgagtcggat cgatcaagcg gtcggtatgg cccgttcccg cgtggtgctg
     8761 gtggagcatg atctgggttg cctcgccgtt gcctggtggg cggcgctgct cgacaagcgg
     8821 gcggcccggc gcgttatcgg cgcgctgctg gttgcacccc ccgatccgga cgcgcccgac
     8881 agcgatgatc gggtccgccg cttcggcccg ctgccggaag cgatgctgcc cttccccgcg
     8941 atcctggtcg ccagccgcga cgacccaaac gcgaccatcg aacggtcccg cgcgatggcg
     9001 gccgaatggg tcacagactt tttcgacgcc ggatcgctcg gccatctcga cgctcgttcg
     9061 cacctcaacg cgtggccgga cggccagagg ctgctcgata tcctgatcga cggcggaccg
     9121 ggcctgtcgc gctacaccta tcggcgaccg cctccgcccc gaccggcgag tcgtccggta
     9181 catcgcatga cgccctcgtc ccagccctga gaagggggga gtcgtgcggg atcgggcaag
     9241 gcgtatgcgt gtcgggccga ggaaccgcga aaatggcgac acatgccgct tatatggaag
     9301 gcttccgcgc cgcgacgctg cgacacagcc gggagaaatg cccctacccc ccggacacga
     9361 gagagcaggc ggagtgggtc gccggatggg aagccggcga cgaccatgac gaagtcggcg
     9421 aatgcagttc ggactgacat gccgatgcac ggcgtgcacc aacgagaggg tcgccgttcg
     9481 cgcggacgtc agatcgaccc caggctgagg cctattaccg ggaacgacgt gagactcgcg
     9541 aaggcgaaca gcgccggcac ggcacggcac ggcaacaaaa tggttccatg aaacgcgatg
     9601 aagcgaacga aatgctgatt gcgcgttgcg cgcgcgatcg cggcgtaaag cgcagcgccg
     9661 gcatcgatac catcggcctg ctcatcgatc gccgcgcaga actcttccat ccatgcctcg
     9721 atggcggcga cgtcggcgcg gcgctcggct gcgaggcccg ccatctcggt ctcgacagcg
     9781 atacgcagct cgagcagggc cagcacatct tcgagatttg gaagctcctt ggcggtcacc
     9841 tggaccaccc gcggagcgac ctttacgaaa acaccggagc cctgccgagc ctcctcccaa
     9901 ccttcagcag acaagcccgc aacagcctcg cggacgacgg tgggaccgat gcgtggggtc
     9961 acttaacgct tctggaagca cgacattatg aagcaggccg aaaaatgtgg gccacgcatc
    10021 aattccacgc ttcacaccat cttcgacaga tgatgccgcc gccaaagcga ggccggtcaa
    10081 cacgtgccac tcacgcttcg cccgcccagc agcaacttgg atagacaacc agcccaacgc
    10141 gccgtgccta gagaatcgga cagtgatcct accaaagcgc cccacgctgg acccacgtgg
    10201 gttatccaca ttcccgcgct ttcgccggat tccgtcgaaa gaagctacga atgatgtctt
    10261 aggaaaatgg tgcgcctaca ggactcggaa tacggtctct aacgtattga tatagcatgt
    10321 actattttca tgccataaat cccgtggccc actcgtggtc cacggcgaaa attggtcctc
    10381 cttgaggccc cgaacctgcg ccggggtata ggactcgaac atcagaatat ggcgacgcaa
    10441 ttgaaccctt ttgatcatct gaatccggta aatgttggcc gcttccatga cgcacccccg
    10501 tcaacttcat cgtccacacc ctcaccgagg tgaacgacgg ccttgcccat ggccgctatt
    10561 tcttcatgga cgtcgccagg gacggcatcg ccctctacga gtccgacagc agcgagctgc
    10621 acacccccaa gcccaagacc cccgcgcagg cgctcgcgat ggcgaaggag tatttcgagg
    10681 agtggtttcc cagtgcgctc gtctacctcg gcaccgcccg cgatctggtc acgcagggcc
    10741 gaaacgagga accagcgttc atcctccacc agtcggccga gcggctctac cattgcgtgc
    10801 tgctggtcgt gaccttctac acgcctcacg tccacaacct gggcttcctg cgcacccagg
    10861 ccgagcgcat cgagaacctc ggccaggtcg tccacgccgt ctgctccgat cgcatcgcca
    10921 agctcgaaca ggacgcccgg caaggctcgc ctgatctacc cgcccaccag cggccgtcgg
    10981 tgccgactca ccacggctcg ccagccgcca tcctcgccgc gatctccgcc tcgttcaccg
    11041 gcacctcggc gcgcgcctcc tcgacgctcc acccgtgcgc gacccgggtc agataataga
    11101 gcgaccatgc aaagcgccgc tcgacctcat ccaccccgac cccgagccga gccgctatcg
    11161 ccccatagcc caggtcttca tagcgcaccg cgccgaacac cgcataaagc acctcgggca
    11221 tcgtatcgac cgcccgctgg agccgacgca accggcgcca cccggacacc catttgccca
    11281 cgccgcaacg tccctccgca gcccccatcc gctccatcat gcggaccttc tcgatcagcc
    11341 tcatcgctcc actccttcct tccctaaacc accacggccc cggacggcgg ggtgggcggc
    11401 aagagcggag cggcagcccc gccgatggag gcggggtgca cccggagggc cgcaacgaag
    11461 tggaggaccg gcgcagccgg ttgcgccgcg ccgcggcggg gctagagggc agcgaccgcc
    11521 cgcaccgctg gccgaaggcc accaacgtca tcgcgcaccg cgcgatggcc gcttgccggg
    11581 ccgcaggcgc ggccccgcgt tagcgaccgt caccgcgcca gcggacgaga taccgaaggc
    11641 ggctcggcag aacgaagcgg cgtagagcgc ggccgcgcgc ggcaacgcca aagcctctga
    11701 gccaggcaag ccagccgcca gcctgttgtt ttcactagat aatatcggat aagccccagc
    11761 cgccctgttc actgactggc ttaattcgta ctgtaaaaac acccacatag gcgcctgaga
    11821 ctggcgcccc ttttatctcg ccgtcaattc ccaagccccc tccgacgccc catgtctcca
    11881 acgacgagca gctaaagaga gaaggggttc aagcgcttag cgctcatccg gcatcaaaac
    11941 ggcaccggga gcctccaccc cagtgccgtc tgtctcccta aaaaacgatg tgcagacaac
    12001 aggttacccc gcagggggag ccgttccttt tatcttgctt tcacacttca gcgcctgtcg
    12061 cttaatagac gaccagccta cctccctgag ggaacggtcc tcgccagccg caagggaagt
    12121 gcccattagc gtaccagtac gactggcttg caaaaaagcc gggcgggtaa acatctgcga
    12181 actcggcttc tacgcaaagg tgcagtatat cccatgctac ggcatttaaa aaatctttgg
    12241 gtagattatt atcttgaatg acgcgctgtg tcttttctcg cgtcagtgcg attgcctccg
    12301 ggcgcacgga caagacaaca tcgttccacc tttcatattc ttcgggcgat cgttctctaa
    12361 tccgatcact atatgcgtta acagcgtcgt ggcacaggcc gacccaggtc ggtgaagagc
    12421 agctctcaac agcttcctgc caagatgaga ctacatctgc cacctccgtg tcacgaacac
    12481 cgacattgcg gaaccagtct tctgcgcgga gcttatcgag ggtggcgacg gttcttggat
    12541 gcatcattca ccttgctctc tgtggtagtc gccccggcac tcgcggcacg agcgtctcgg
    12601 gcgtcagccc aaaactagca cgctggcctg cggcttgcgc gctaatactc gcggcctgcg
    12661 atggtgtgta gccagattgg accaaggcct tttcaacagc tgcaccgtat tcgccattgg
    12721 tcggccggag accaaaaaga tcgctgtc
//
LOCUS       DATGEM010000535         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_908916, whole genome shotgun sequence.
ACCESSION   DATGEM010000535 DATGEM010000000
VERSION     DATGEM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908916"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VKQ27_16320"
     CDS             complement(<1..1271)
                     /locus_tag="VKQ27_16320"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY90549.1"
                     /translation="MKDPAWALAEHVCGCSLADLPAATRRATCNDILDTFGCLLGGSG
                     EPGIAELVRIVGGWGGAEQSQVMLRQRRLPAPHAALMNASMAHALDFDDTLDHGGSIH
                     PGASVLGASLAVSDLLGGVPGQDLLLAIALGLDVSCRIALASTLDRGWHRTAAIGVFG
                     AAAASGKLLGLSAEQMVNALGIAFSQAAGNRQCIVDGALTKRLQAGQAASSGVLSALL
                     AREGFTGAHNIFLGRFGFFELYQPNGYDAAKLTHDLGHRLRGDELSFKPYACGRPQHA
                     MLDAAIAVREQLQLGTSVALGEIAEVSVTGPAAMMDEASFDAAHKRRPTQIVEAQFAL
                     PYLIATAIVHGRVGITEVADIHHAVVLELAARITGNPSGQKASGVTIHLRNGRTATAK
                     VGPPLGSPENRMSIEQLTMKFSACARHAVQPL"
     gene            complement(1300..2145)
                     /locus_tag="VKQ27_16325"
     CDS             complement(1300..2145)
                     /locus_tag="VKQ27_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY90550.1"
                     /translation="MRNTHIELSPIAGALGAEIGGVDVARDLDDGVIREIRQALLDYG
                     VIFFRDQNLERDQHKAFTRRFGKIFIHPNYNGVSADPEIVDIRREPGDKKIVGEDWHT
                     DTTMVEEPPMGAILYAIEVPPYGGDTLFANQYLAYEALSPGLKRTLEGLRAVHTDRMV
                     AGPQANMNAHRSTKVREDASWRETISVHPVVRTHPETGRKLLFVNRSYTVGFEGWTEA
                     ESRPLLEYLLEHGHRPEFTCRFRWANGSIAFWDNRCTKHLAVHDAGPFRRIMRRTQIR
                     GDAVH"
     gene            2234..2581
                     /locus_tag="VKQ27_16330"
     CDS             2234..2581
                     /locus_tag="VKQ27_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HLY90551.1"
                     /translation="MPDRVVYKVMSAAELEQMRRDGAFHGSLVDVADGFVHLSCGSQL
                     AETLDKHFSGVDGLMLIAVDLSRLGNAVRWEPSRGGQLFPHIYGELPLDAVVAVAAVE
                     RTADGAVKLPGSA"
     gene            2835..3188
                     /locus_tag="VKQ27_16335"
     CDS             2835..3188
                     /locus_tag="VKQ27_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLY90552.1"
                     /translation="MNLSCLTSRTRRRAAMITGIILAGLPLGAAAQETGDPAEGRHLA
                     GTFCSNCHIITPTKQAVGSNHVPTFSAVANRPSTTPASLRKSMLRPHPQISNLQATPD
                     ELRDLIAYILSLRGK"
     gene            3415..3639
                     /locus_tag="VKQ27_16340"
     CDS             3415..3639
                     /locus_tag="VKQ27_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90553.1"
                     /translation="MSETVDALLCDMLDWLARGARPYDEVMDAWRTSCPRLPVWEDAN
                     DRGFVEQARLNGCAMVRVTAEGRAFLARRG"
     gene            3709..4503
                     /locus_tag="VKQ27_16345"
     CDS             3709..4503
                     /locus_tag="VKQ27_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY90554.1"
                     /translation="MPKNYDTLQVTTPQPHTLLVKLNRLEVANAMNTQMGLDLLDLFH
                     TIGGAPNEQRCIVITGAGERAFCAGGDLKERNGMTDQQWQDQHLIFERAIRAIIHCPV
                     PVIAAVNGAAYAGGMEISLCADFIYAAEHARFALTEVTLGIMPGAGGTQTLPRAVGGR
                     RAKEILLTGKPFTAEQAFEWGMVNRICKAGTLLAEAMETATTIATNAPISTRQIKQSV
                     NYGLNMDLASGMMFEIEAYNRMVPTEDRREGIRAFNEKRKPQFQGR"
     gene            4515..5882
                     /locus_tag="VKQ27_16350"
     CDS             4515..5882
                     /locus_tag="VKQ27_16350"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY90555.1"
                     /translation="MNAITDIAAQGLTRALAEQARTLTYADLPEDVRKLARQCLVDYV
                     ACTLAGSKDELAAILLAEMREQGGAPIATVIGHSARLPVLSAAVVNGASSHALDYDDV
                     NMAMTGHPSVVLLSALLALAAERGSSGQDVMTAFVAGYELQCRLGLALAPGHYNALGF
                     HATGTLGSFGAAAACAHLLGLDEAQFAAALGIAGTQAAGLKSLFGTMCKPLHAGKAAY
                     HGALAARLALRGFTARGDIIECAQGFARTHSPDWHPERALAAPPRGFHILNNLFKYHA
                     ACYMTHAPIEAARKLREQHGLRPDQIASIRLGLDETCDRICNIPTPRTGLEAKFSLRL
                     TTAMALAGVDTGGLESYSEETAADPTLIALRDKVEFDFRTGVSNTVADMELVLTDGRS
                     VSTSYDSGVPASDIDEQGGRIAEKFSSLAEPMLGRAKANDLLGQVSRVDQLADLRGVM
                     RLCAV"
     gene            5939..6322
                     /locus_tag="VKQ27_16355"
     CDS             5939..6322
                     /locus_tag="VKQ27_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLY90556.1"
                     /translation="MASVKDPNDTRGRLIAADQVEGTSIYNAKGESLGSVEDVMIDKV
                     SGKIAYAIAGFGGFLGIGNRHYPLPWDKLKYDTRMGGYVVDLDRRTLEGAPSYADTDV
                     AGWQDPAWGRRVYDYYGMPPYSGMP"
BASE COUNT         1223 a         2076 c         2240 g         1237 t
ORIGIN      
        1 agcggctgca ccgcatggcg cgcgcaggcg gagaacttca tcgtgagctg ctcaatgctc
       61 atgcgattct ccggcgagcc gagagggggc cctaccttag cggtcgcggt gcgaccgttg
      121 cgcaaatgga tcgtgacacc gctcgccttt tgtccggagg ggttgccggt tattcgcgcg
      181 gccagctcca gcaccacagc atgatggata tccgcgactt ccgtgatgcc aacgcgccca
      241 tgcacgatcg ccgtggcgat cagatatggc agcgcgaact gtgcttccac gatctgcgtt
      301 ggccggcgtt tgtgggcggc gtcgaatgat gcctcgtcca tcatcgctgc cgggccagtg
      361 acgctcacct cggcgatctc gcccaaggca acggacgtgc cgagctgcaa ctgttcacgt
      421 actgcgatcg cagcatcgag catcgcgtgc tgagggcggc cgcacgcata tggcttgaag
      481 ctcagctcgt caccgcggag tctgtgcccc aggtcatgcg tgagcttggc cgcatcgtaa
      541 ccgttcggct ggtagagctc gaagaaaccg aaccggccca ggaatatgtt gtgcgccccg
      601 gtgaacccct cgcgcgccaa caacgcggac aacacgccgc tgctggcagc ctggcccgcc
      661 tgcaagcgct tggtcagcgc gccatccacg atgcactgac ggttcccggc agcctgactg
      721 aacgcgattc caagcgcatt caccatctgc tccgcgctca gcccaagcag cttgccggat
      781 gcggcggcgg cgccgaacac accgatggcg gccgtgcgat gccagccacg atcaagtgtc
      841 gaggcaaggg cgatgcgaca ggaaacatca agcccgagtg cgatcgccaa cagcaggtct
      901 tgtcccggta cgccgccgag caggtcggaa accgcaaggc ttgcccctag caccgacgcg
      961 ccgggatgga tcgacccacc atgatccagc gtgtcgtcga aatcgagggc gtgcgccatg
     1021 ctggcgttca tcaacgctgc atgcggtgcc ggcagccgtc gctgccgcag catcacctgg
     1081 ctctgctccg cgccgcccca gccaccgacg atgcggacaa gttcggcgat accaggctcg
     1141 ccgctaccgc cgagcaggca gccgaacgtg tccaggatgt cgttgcacgt ggctctgcga
     1201 gtcgcagcgg gcaggtctgc gaggctgcat ccgcatacat gctcggccaa tgcccatgct
     1261 ggatctttca ttggtgtcct gtcggcgagt tggaggcggt cagtgaacgg cgtcgccgcg
     1321 gatttgcgtg cggcgcatga tgcggcggaa cgggccagca tcgtgcaccg ccagatgctt
     1381 ggtgcagcga ttgtcccaga atgcgatgga gccattcgcc cagcggaagc ggcaggtgaa
     1441 ctccggccga tggccgtgtt ccagcagata ttccagcagt ggcctgctct cggcctctgt
     1501 ccaaccttcg aagccgacgg tgtaggagcg gttgacgaac agcagcttgc ggccggtttc
     1561 cggatgcgtg cgtaccaccg ggtgtacgct gatggtttcg cgccagctgg catcctcgcg
     1621 caccttggtc gagcggtgtg cgttcatgtt ggcctggggg ccggcaacca tgcggtctgt
     1681 gtgcacggcc ctgaggcctt ccagcgtgcg cttcaggccg ggcgagagtg cttcgtaggc
     1741 gagatattgg ttggcgaaca acgtatcgcc gccatagggc ggcacctcga tggcgtagag
     1801 gatggcgccc atcggtggtt cctccaccat cgtggtgtcg gtatgccagt cctcgccgac
     1861 gatcttcttg tcgccgggct cgcggcggat gtcgacaatc tcggggtccg cgctgacgcc
     1921 gttatagttg gggtggatga agattttgcc gaagcggcgg gtgaaggctt tgtgctgatc
     1981 cctctcgagg ttctggtcgc ggaagaagat cacgccgtag tcgaggagcg cctggcggat
     2041 ttcgcgtatc acgccgtcgt cgaggtcccg cgcgacatcg acgccgccga tctcggcgcc
     2101 gagtgcgccg gcgatcggcg aaagttcgat gtgagtatta cgcattggtg gctcctcctt
     2161 gggcaaacgt tgagcaatgc ggtggagtgg gtcaagttgg acaacgccgg ggaagcgagg
     2221 tgagcgaggg gccatgccgg atcgggtcgt ctacaaggtc atgtcggctg cggaactgga
     2281 gcagatgcgg cgagacggtg cattccacgg ttcgttggtt gacgtcgctg atgggttcgt
     2341 gcacctgtca tgcggctccc aacttgccga gacactggac aagcacttca gcggggtcga
     2401 tggccttatg ctgatcgcgg ttgacttgtc gcggctcggc aacgccgtgc gctgggaacc
     2461 gtcacgcggc ggacagcttt ttccgcacat ctatggcgaa ttgccgttgg acgccgtggt
     2521 cgcggtcgct gcggtcgagc gaaccgctga cggtgcagtg aagttgccgg gatcggcttg
     2581 aaacgatcca aggtccgcgt ataccgtgtc ggcgatccca ggtcggggga gcaggttgcc
     2641 ctatccgaca cggtcgatgc gctgctgcgt gacatgctgg ggtggctggc tggcgagtgg
     2701 tgctcacacg tatccggccc ttccctcgcc actcgtacga gcggctgtgc cacaggcgca
     2761 ttgcttaaga gcggttaacg gcggatactg cttcagatac ttgcacctga aaatgcggga
     2821 gatttgttag ggccatgaac ctgagctgtc tcacctcacg aacgcggcgt cgtgcggcaa
     2881 tgatcactgg catcatcctc gctggtctgc cgcttggcgc ggccgctcag gagaccggcg
     2941 atcccgccga gggaagacac ctggctggga cgttttgcag caactgccac attatcaccc
     3001 caacgaaaca ggctgtcggc agcaaccacg tgccgacatt ctccgcggtt gccaaccggc
     3061 catcgaccac gccggcatcg ttgcgtaaat ccatgctgag gccacatcct cagatttcga
     3121 acctgcaggc gacaccggat gagctccgcg atcttatcgc ctacatactg agcctgcgcg
     3181 gaaaatgatc tgacgcagaa gcgtgcggcg aatgtgaatt tcgcttggaa tgaccggcgc
     3241 agatcctgcc gtccagcctt cacaggcgac agcgcctctc ctggcatcag ccattcgccg
     3301 ccgcgagccg atcgtccgct cccgagccgc caccgtagcg gcttgaatca tccatacctg
     3361 gctcgtagat cagtctctat cgggactgtc cccaagccac aaaggaggac gcggttgtcc
     3421 gagacagtcg atgccctgct gtgcgacatg ctcgattggc tggcgcgcgg tgcccgtccc
     3481 tatgatgagg tgatggatgc ctggcgcacg tcatgcccac gcctgccggt gtgggaagac
     3541 gccaacgacc gtggcttcgt ggagcaggct cggttgaatg ggtgcgcgat ggtgcgggtc
     3601 acggcggaag ggcgcgcgtt cctggcgcgg cgcggatagc gcggccacat tgcgggcggg
     3661 cgcacgcgcg cgcagtgtgc gcccctgatc gagacaccgg aaccgctcat gcccaagaac
     3721 tacgatacgc tgcaggttac cacgccgcag ccccatacgc tgctggtgaa gctgaaccgc
     3781 ctcgaggtcg ccaacgcgat gaacacgcag atggggctgg atctgctgga cctgttccac
     3841 actatcggcg gtgcgccaaa cgagcagcgc tgcatcgtta tcaccggcgc cggcgagcgc
     3901 gcattctgcg ccggcggaga cctgaaagag cgcaacggca tgaccgacca gcagtggcag
     3961 gatcagcatc tgatcttcga gcgtgccatc cgcgccatca tccactgccc cgtaccggtg
     4021 atcgccgcgg tgaacggggc ggcctatgcg ggcgggatgg agatttcgct gtgcgccgat
     4081 ttcatttacg ccgctgagca tgcgcggttc gcgctcaccg aagtcacgct cggcatcatg
     4141 cccggcgccg gtggcacgca aaccctgcct cgcgcggtgg gagggcgacg ggcgaaggag
     4201 atcctgctca ccgggaagcc gttcaccgcg gagcaggcgt tcgaatgggg tatggtcaat
     4261 cgtatctgca aggccggcac gctgcttgcg gaagcaatgg agaccgccac gacgatcgcc
     4321 accaacgcgc cgatttccac gcggcagatc aagcagtcgg tgaactatgg gctgaacatg
     4381 gacctcgcca gcggcatgat gttcgagatc gaggcctata accgcatggt gccgacggag
     4441 gaccgccgcg aaggcatccg cgccttcaac gaaaagcgca aaccacagtt ccagggacgc
     4501 tgaggaggaa acgcatgaac gccatcaccg atatcgccgc acagggcctg acgcgggcat
     4561 tggccgagca ggcgcgcacg ctgacctacg ccgatctgcc ggaggatgtg cgcaagctgg
     4621 cgcggcaatg tctggtggat tacgtcgctt gcacgctggc cggatcgaag gacgagttgg
     4681 cggcgatcct gttggccgag atgcgggagc agggcggcgc cccgatagcc acggttatcg
     4741 ggcacagcgc acggctgccg gtgctgtcgg cggcggtggt gaatggtgcc tcgtcgcatg
     4801 cgctcgacta tgacgatgtg aacatggcga tgaccgggca tccaagcgtg gtgctgctgt
     4861 cggcactgct cgccctggcg gcagagcgcg gcagctctgg tcaggacgtg atgaccgcgt
     4921 tcgttgcggg ttatgagctg caatgccgtc tcggattggc gctggcgccg ggccattaca
     4981 atgcgcttgg gttccacgcc accggcacac ttggcagttt cggtgccgcc gcggcgtgcg
     5041 cgcatcttct ggggttggat gaagcacagt tcgctgccgc gctgggcatc gcgggaaccc
     5101 aggcggccgg gttgaaatcg ctgttcggca cgatgtgcaa gccgctgcat gcgggaaaag
     5161 ccgcgtatca tggcgctttg gcggcacggc tggcgctgcg cggcttcacc gcgcggggtg
     5221 acatcatcga gtgcgcgcaa ggcttcgccc gcacgcacag tccggactgg catccggaac
     5281 gcgcgctcgc cgcgccgccg cgcggattcc acatcctgaa taacctcttc aagtatcatg
     5341 ccgcctgcta catgacgcat gcgcccatcg aggccgcgcg gaaattgcgc gaacagcatg
     5401 gtctgcggcc cgatcagatt gccagcatca ggcttggcct tgatgagacg tgcgatcgga
     5461 tctgcaacat tcccacgccg cgcaccggcc tggaagcgaa gttcagcctg cggctgacga
     5521 ctgcgatggc gctggccggt gtggatacgg gtgggctgga gagctactcg gaggaaactg
     5581 cagcggatcc cacgctgatc gcgctgcggg acaaggtcga gttcgatttc cgcaccggtg
     5641 tttcaaatac ggttgccgac atggaactgg tgctgacgga cgggcgaagc gtgagcacgt
     5701 cgtacgactc cggtgttccg gcgagcgaca tcgatgaaca gggcggccgg atcgcggaaa
     5761 agttttcttc ccttgcggaa cccatgctgg ggcgcgcgaa ggcgaacgat ctgctgggcc
     5821 aggtcagccg ggtcgaccag ttggctgatc tccgcggcgt gatgcggctc tgcgcggtgt
     5881 aggcaaccgc ttactgttct gcacgttatc caggcctcca caacggaaga ggtcgaatat
     5941 ggctagcgtg aaggacccca atgatacgcg cggcaggctg atcgctgcgg atcaggtcga
     6001 gggtacgtcg atctacaacg ccaagggcga aagcctcggc agtgttgaag acgtgatgat
     6061 cgacaaggtc agtggcaaga tcgcgtatgc aatcgcgggc ttcggcggct tcctgggcat
     6121 cggcaaccgg cactatccgc tgccgtggga caagctgaaa tacgatacgc gcatgggcgg
     6181 ctatgtggtc gaccttgacc gcagaaccct ggaaggcgcg ccttcctatg ccgacacgga
     6241 cgtagcgggt tggcaggatc cggcgtgggg ccggcgcgtc tatgactatt acggcatgcc
     6301 gccttactca gggatgccgt aaccttccgg tgcacgacgc agcggcggcc ttcgggccgc
     6361 cgtttgcatg tacgcggccc tgacatgggg cgcgcgcgta atctaccaac cctatcgcat
     6421 ccccaacttg ccggcgtccg atgctaccga tcgcaatata gggaggcgat cccggcaaaa
     6481 tggggaaccc ctcccgatgc ccgtattgat tgcggaccct agcttcacag ggtcaacgca
     6541 gttccgcgtg cgtggaaggg caaggccgtg ttctcgagtc ggaaatattc cgtcctagta
     6601 gcgtggcagt gccgcagatc actggccagc ccgaacagat ggtctatcgc cccaacatgt
     6661 gaactccgcc ggaaccgcgt cgccgatgca ttacgcgctg ccatagccgc aggcaatcgc
     6721 cgcagatttt ggcggcttca gtgatgtgat cggtccatca gttgcagact caacac
//
LOCUS       DATGEM010000536         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_260821, whole genome shotgun sequence.
ACCESSION   DATGEM010000536 DATGEM010000000
VERSION     DATGEM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260821"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VKQ27_16360"
     CDS             <1..554
                     /locus_tag="VKQ27_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90557.1"
                     /translation="LLMVGLATIVLAGDLIYLRRQRMLRVIWTWLIIAPVLFLLGLTF
                     VEPWVGGAEVKTMMPASEIGEFFGDSYFRHTGQPLTAVAGDPELAALIGYSASRRPHL
                     LLDETPDRTPWLTIARFNEAGGLVVWRAADTSGTPPEDIARHFPGLVPEVPRSFTRYL
                     NGRQPLLRIGWGIIRPKATASSQ"
     gene            642..1967
                     /locus_tag="VKQ27_16365"
     CDS             642..1967
                     /locus_tag="VKQ27_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HLY90558.1"
                     /translation="MAEQERGQTLLERQFGLTASGTSIRTEFVAGLTTFLTMVYIVFV
                     NPLILGNAGMDKGAVFVATCIAAAVSTVVMALYANYPIALAPGMGLNAFFAFTVVIAD
                     KYSWQQALAAVFCSGVLFFLLSIFRIREYIINAIPRNLKFAISAGVGLFLGIIALEQA
                     EIVVASQATLVTLGPLSGAGSLAPLLCLLGFVLIVALNARGIIGGTLIGILIVALIGL
                     PLGLTSFGGIVSLPPSLAPTLLKLDFSRVTELSFIVIIFSFLFIDVFDNAGTLIGVTH
                     RAGLTDKDGNLPRMKQALISDSFAAMFGALIGTSTTTSYIESAAGVSAGGRTGLTAVF
                     VALFFLLALFFAPLAGMIPAYASSAALLYVACVMTRGLAEIDWSDATEYAPAVVAAIT
                     MPLTYSIATGIGLGFITYAVAKLVAGKFADAKPAVLALAVLFAVKFAVG"
     gene            1989..2393
                     /locus_tag="VKQ27_16370"
     CDS             1989..2393
                     /locus_tag="VKQ27_16370"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY90559.1"
                     /translation="MALNGSCLCGTVRYEIDQLDTPIGHCHCTTCRKAHAAAFASTAR
                     VTRSHFRWRGGQDALGAFESSPGRFRRFCARCGSQILAERLEDPHIVLRVATLDDDPG
                     VRPVVHIFIADAAPWLDEGDDLPRYLTWPPGR"
     gene            2375..>2576
                     /locus_tag="VKQ27_16375"
     CDS             2375..>2576
                     /locus_tag="VKQ27_16375"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLY90560.1"
                     /translation="MAARAITPLVVLISSGDSSPNQKQTWEVAVKVGFIGVGSMGGPM
                     SRNLVSKSNHEVTVFDLNARAIE"
BASE COUNT          411 a          823 c          819 g          523 t
ORIGIN      
        1 cgctgttgat ggtggggctc gcgaccatcg tgctcgccgg cgatctcatc tatctgaggc
       61 ggcagcggat gctgcgggtg atctggacct ggctcatcat tgcgcctgtc ttgttcctgc
      121 tcgggttgac cttcgtcgag ccgtgggtcg gaggcgccga ggtcaagacc atgatgccgg
      181 cgtccgagat cggagagttc ttcggcgaca gctatttccg tcataccggc cagccgttga
      241 ccgcggtcgc cggagatccc gagctcgccg cgctgatcgg ctacagcgcg tcgcgccgtc
      301 cacatctgct gctcgacgag acgcccgacc ggaccccctg gctgacgatc gcccgcttca
      361 atgaggccgg gggactggtg gtctggcgtg ccgccgacac ctccggcacc ccgccggagg
      421 acatcgcccg tcactttccg ggcctggtgc cggaagttcc gcgctcattc acgcgctatc
      481 tcaacgggcg gcagccgctg ctccggatcg gctggggcat catccgcccg aaggcgacgg
      541 catcgtcgca gtagcagcgc cagcgcagtg gaaaatcgcc gccaataagc ggacgggcgc
      601 cggataagga ttatgctgcg ctgtcttaca cggagggggc gatggcagag caggaacggg
      661 gccagacact tcttgagcgg cagtttgggc tgacagcatc agggacctcg atacgcaccg
      721 agttcgttgc tggcctcacg acgttcctga cgatggtcta catcgtgttc gtcaacccgt
      781 tgatcctcgg caatgccggg atggataagg gcgcggtgtt cgtcgcgacg tgcatcgcgg
      841 cggcggtctc gacggtcgtg atggcgctct acgccaacta cccgatcgcg ctggcgcccg
      901 gcatggggct gaacgcgttc ttcgcgttca ccgtggtcat cgccgacaag tatagctggc
      961 agcaggcgct ggctgcggtg ttctgctcgg gggtgctgtt cttcctgctg tcgatcttcc
     1021 gcatccgcga atacatcatc aacgccattc cgcggaacct gaagttcgcg atctccgccg
     1081 gtgtcggcct gtttctcggc atcattgcgc tggagcaggc cgagatcgtg gttgcgagcc
     1141 aggcgacgct ggtgacgctc ggaccgctat cgggagcggg cagccttgcg ccactgttgt
     1201 gcctgctcgg cttcgtgctg atcgtcgcgc tcaatgcgcg cgggatcatc ggcggcaccc
     1261 tgattggcat cctcatcgtc gcattgatcg gtttgccgct gggactgaca agtttcggcg
     1321 gcatcgtatc gttaccacca tcgcttgcgc ccaccttgct caagctagat ttctcccgcg
     1381 taaccgagct gagcttcatt gtcatcatct tcagtttcct gttcatcgac gtgttcgaca
     1441 atgccggcac gttgatcggg gtaacccatc gcgccggcct caccgacaag gacggcaacc
     1501 tgccgcggat gaaacaggcg ctgatctcgg acagctttgc cgcgatgttc ggggcgctga
     1561 tcggtacctc gacgaccacg agctacatcg aaagcgccgc cggcgtatcg gccggcggcc
     1621 gcaccgggct gaccgccgtg ttcgtcgcgc tattcttcct gctcgccctg ttctttgcac
     1681 cgctcgccgg gatgatcccg gcctatgcgt catccgccgc gctgctctac gtcgcctgcg
     1741 tcatgacgcg cggcctggcc gagatcgatt ggagcgacgc cacggaatat gcgccggccg
     1801 tggtcgcggc gatcaccatg ccgctgacgt attcgatcgc gaccgggata ggtctcgggt
     1861 tcatcaccta cgcggtggca aagctcgtcg caggcaagtt cgcggatgcg aaaccggccg
     1921 tgctcgcgct cgcggtgctg ttcgcggtaa aattcgcggt ggggtgacgc tagaactgga
     1981 ggtaacacat ggcactgaac ggtagctgcc tgtgcggcac agtacgctac gagatcgacc
     2041 aactcgatac gccgatcggt cactgccatt gcacgacgtg ccgcaaggct cacgcggcag
     2101 cctttgcctc cactgcgcgg gtgacccgct cgcattttcg ctggcgcggc ggtcaggacg
     2161 cgctcggcgc cttcgaatcc tcgcccggcc gcttcaggcg cttttgcgca cggtgcggca
     2221 gccagattct cgccgaacgg ctcgaggatc cgcacatcgt gttgcgggtt gcgaccctag
     2281 acgacgaccc cggcgtgcga ccggtggttc atattttcat cgccgacgca gcgccgtggt
     2341 tggacgaagg tgacgacctg ccgcgctatc tgacatggcc gcccgggcga taacgccact
     2401 ggtcgtgctc atttcgtcgg gtgatagttc tcccaaccaa aagcaaactt gggaggtcgc
     2461 agtgaaagtc ggatttattg gcgtcggcag catgggcggg ccgatgtccc gcaatctggt
     2521 ctccaaatcc aaccacgagg tcaccgtttt tgacctcaat gccagggcca tcgagg
//
LOCUS       DATGEM010000537         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1630742, whole genome shotgun sequence.
ACCESSION   DATGEM010000537 DATGEM010000000
VERSION     DATGEM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630742"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VKQ27_16380"
     CDS             complement(<1..244)
                     /locus_tag="VKQ27_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HLY90561.1"
                     /translation="MAPAGTSAPGAPTQAGTPHTLAEALAATYATQPALLAERAKLRA
                     TDENVPKALSGWRPQVILSGQGGYGDGFSRIYSGASG"
     gene            complement(426..1079)
                     /locus_tag="VKQ27_16385"
     CDS             complement(426..1079)
                     /locus_tag="VKQ27_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLY90562.1"
                     /translation="MDLAEARNRMVDSQVRPNKVTDPRIIAAMRRLPRERFVPPGQAV
                     RAYADEDVPLGEGRVLIEPMVMARLLQLTAVSAGERVLVVACGSGYGAAVLAGCGARV
                     TALEDAPALLAVAREALAEFAPSVNLVSGPLAAGWPTGAPYDVILIEGAVRSIPNSIG
                     EQLHQETGRLVAVCTDGNGTSQAVLAEATVAGLRTQPMFDCATPVIPSLLPAPGFVF"
     gene            complement(1149..1745)
                     /locus_tag="VKQ27_16390"
     CDS             complement(1149..1745)
                     /locus_tag="VKQ27_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLY90563.1"
                     /translation="MLPRHTDPALARAVRWVALLNLGYFGIEFAVAIGIGSVSLFADS
                     IDFLEDASVNLLIAVALGWTQRRRARVGMLLAGVLLVPGLATLWTAWTKFSHEAAVPA
                     ALPLSLTGLGALVVNLSCALMLARYRHHAGSLTRAAFLSARNDVLANIAIIVAGAVTA
                     LVWHSAWPDLLVGLGIVVMNSDAAREVWTAARDEHAEG"
     gene            1836..1909
                     /locus_tag="VKQ27_16395"
     tRNA            1836..1909
                     /locus_tag="VKQ27_16395"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Cys,seq:gca)
     gene            complement(2064..>3440)
                     /locus_tag="VKQ27_16400"
     CDS             complement(2064..>3440)
                     /locus_tag="VKQ27_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY90564.1"
                     /translation="LVSALRDACGVDFIIGLKLPGDDGVHGSIGPAEAAIIGDLLTRK
                     REVDYVCFAGGAHARTLEMHTPDRHGPPMPYMGFIKELRHHLNGVPLMALGRITDPAE
                     ADGILSRGEAALIALGRPLVADPAWPRKAAQGRTWDIRYCLSCNTCWGAIIMMHVPIA
                     CVNNPRVAQPDEVDFAPRRAERRKRVVIVGTGIAGLEAAWVAAARGHEVTVLGASDHL
                     GGKAWLREQLPGGETVSSIYDYQTTAARRAGARFELGRRVTAAEIIAAEPDAVVLATG
                     SRMIPPAWLPADVLQEGMVGDLRACLPEVLRHDGRQPGTAVLFDTDHTEGTYAAAEAL
                     RHRFERMLIVTPRDTIANDVQMVTRQGILRRMAEQHITVVPLSEPRWSDGCEEGQLEI
                     VNLYNGDVILVEDLALLTYATPRVPDDVLAEPLRAAGITVIAVGDARAPGEMLFATAS
                     GNAAGLAV"
BASE COUNT          632 a         1227 c         1049 g          532 t
ORIGIN      
        1 cgcctgatgc gccagaatag atgcgggaaa atccgtcacc atacccgccc tgccccgaca
       61 aaataacctg cggtcgccat cccgacagtg ccttgggaac attctcgtcg gtggcccgca
      121 gcttggcccg ctccgccagc agcgccggtt gcgtcgcgta ggtggccgcc agtgcctcgg
      181 ccaaggtgtg gggcgtgccc gcctgggtcg gcgcaccggg tgcggacgtg ccggcaggcg
      241 ccaatggcgc tgccttgacg gtaggctttg ttggtccttg gccaaaggca ggtgcgacgg
      301 cgaaagcgac gcccaagcca accatgcaac cacgcaagac gttcatagct gctcctcttc
      361 tcgcctacct ggagccagcc gctgatgtgc cacagcgggc aaccgaacgc cagaagccag
      421 gacaatcaga agacgaaccc cggagccggc agcaggctgg gtatgactgg cgtggcacaa
      481 tcgaacatcg gttgcgtccg cagtccggca acagtcgcct ccgcgagcac agcctggctg
      541 gttccattgc catccgtgca cacagccact agccgcccgg tctcctgatg aagctgctca
      601 ccgatcgaat tcggaatcga acgcacggca ccctcaatca ggatcacgtc gtagggagca
      661 cccgttggcc atccggcggc gagcggccct gacaccaggt tcaccgacgg cgcgaattcg
      721 gcgagcgcct cgcgcgctac ggcaagcaat gccggggcat cttcgagtgc cgtgacccgg
      781 gcgccgcacc cggcaagcac ggcagcgccg tagccactcc cgcacgccac gaccagcacg
      841 cgctcacccg ccgacaccgc ggtcagttgc agcaggcgtg ccatcaccat tggctcgatc
      901 agcaccctcc cctcgcccaa gggcacgtcc tcatcggcgt aagcccgcac cgcctgaccc
      961 ggcggcacga agcgctcgcg cggcaaccgg cgcatggccg cgatgatccg cgggtcagtc
     1021 accttgttgg ggcggacctg gctatccacc atgcggttgc gggcttcggc aaggtccatg
     1081 gagggctcgt tgctgaggga gggtccgctt ataggggccc cgacaccaat ccgccaagcg
     1141 ccgctctatc agccctccgc atgctcgtca cgtgccgccg tccagacctc gcgcgcggcg
     1201 tccgagttca tcaccacgat acccagtccg accagcaggt cgggccaggc cgagtgccaa
     1261 accagcgcgg tcaccgcccc cgccacgatg atcgcgatat tcgccagcac atcgttgcgc
     1321 gccgacagga acgccgcccg cgtgaggctg ccggcgtggt ggcgatagcg cgccagcatc
     1381 agcgcacacg acaggttcac caccagcgcc ccgagccccg tcagtgacaa gggcaacgcc
     1441 gccgggaccg ccgcctcgtg actgaacttg gtccaggcgg tccaaagcgt cgccagcccc
     1501 ggcaccagca gaacgccggc cagcagcatg ccgaccctgg cgcgcctgcg ctgcgtccag
     1561 ccgagcgcga cagcgatcag caggttgacc gaggcgtcct cgaggaagtc gatgctgtcg
     1621 gcgaacagcg aaaccgagcc gatgccgatg gcaacggcga actcaatgcc gaaatagcca
     1681 aggttgagca gcgcaaccca tcgcacagcg cgggccaggg cgggatctgt gtgtcgtggc
     1741 agcatggtcc gccccttagc atacgccggg ccattgcgcg cggcacttga ccgcgtgacc
     1801 cgtggcaccc gatatacgcc ccctcccgca cggccggtcc ggtggcagag tggtgatgca
     1861 gcggactgca aatccgcgaa tgtgggttcg attcccgccc ggacctccat cgtttcaaca
     1921 ttttagcagg cgttcctcga tcccaatgcc cggcagggat cgtattggcg gtggtggcgg
     1981 attcccgcta tcgctcgata tactgcgccg gtcgatgttg ctccccgaag cgtcgccata
     2041 tccaaactga gttgcgccag gacctagacg gcgagtcccg ccgcgttccc gctggcggtc
     2101 gcgaacagca tttcgcccgg agcccgcgca tctcccaccg caatcaccgt gataccggca
     2161 gcacgcaacg gctcggcaag cacatcatcc ggcacccgtg gtgtcgcata ggtcagcaac
     2221 gccaggtcct cgaccaggat gacgtcacca ttgtagagat tgacaatctc cagttgcccc
     2281 tcttcacagc catcgctcca cctcggctcg ctcagcggaa caaccgtgat gtgctgctca
     2341 gccatccggc gcaggatgcc ctgacgggtg accatctgca catcgttcgc gatcgtgtcg
     2401 cgcggcgtca cgatcaacat gcgttcgaac cggtgacgca acgcctcggc cgcggcatag
     2461 gtcccttcag tgtggtcagt gtcgaacagc acagctgtgc caggctgacg tccgtcatgg
     2521 cgcaacacct caggcaagca ggcccgcaag tccccaacca tgccttcctg gagcacgtcc
     2581 gcaggcaacc aggccggagg gatcatccgc gagcccgtcg ccagaaccac agcgtccggt
     2641 tcagcggcaa tgatctccgc ggcagtcacc cgccgcccca gctcgaaccg tgcaccggcg
     2701 cgccgcgccg ccgtcgtctg gtaatcatag atcgaggaca cggtctcgcc gccaggcagt
     2761 tgttcgcgca gccacgcctt gccgccgaga tgatccgacg cacccaacac cgtgacctca
     2821 tgcccccgcg ccgcggcgac ccatgcggct tccaatcccg cgatgccggt ccccacgata
     2881 acgacacgct ttcgccgctc ggcacgtcga ggcgcgaaat ccacttcatc aggctgcgcg
     2941 accctcgggt tgttcacgca cgcgatcggt acgtgcatca tgatgatcgc gccccaacag
     3001 gtgttgcacg acaggcagta gcgaatgtcc cacgtgcgcc cttgtgccgc cttgcgaggc
     3061 caggcggggt cggccaccag cggccgtcca agtgcgatca gggcggcctc gccgcgggag
     3121 agaatgccat cggcttcggc cgggtcagtg atgcgtccca atgccatcag cggcacgccg
     3181 ttcaggtgat ggcgaagttc cttgatgaag cccatgtagg gcatgggcgg gccatgccga
     3241 tcgggtgtgt gcatctccag cgtgcgggcg tgggctccgc cggcgaaaca gacgtaatcg
     3301 acctcgcgtt tgcgcgtcag cagatcgcct atgatggcgg cttctgccgg gccgatactg
     3361 ccatgcacgc cgtcatcgcc gggcaacttc agaccaatga tgaagtccac gccgcacgcg
     3421 tcgcgcaatg cagacaccag
//
LOCUS       DATGEM010000538         7826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_669954, whole genome shotgun sequence.
ACCESSION   DATGEM010000538 DATGEM010000000
VERSION     DATGEM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669954"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VKQ27_16405"
     CDS             <1..336
                     /locus_tag="VKQ27_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90565.1"
                     /translation="APFTATSMIGFIALAGIIVRNSILLVDFVRHGGGAGRTLRETLL
                     TAGAIRFKPILLTALSAMIGAATILTDPIFQGLAISLLFGLASSTLLTVLVIPAIYVV
                     LRDADRVMP"
     gene            468..1616
                     /gene="dinB"
                     /locus_tag="VKQ27_16410"
     CDS             468..1616
                     /gene="dinB"
                     /locus_tag="VKQ27_16410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLY90566.1"
                     /translation="MVMSPIRKIIHIDMDAFYASVEQRDDPRLRGQPVAVGHGSKRGV
                     VAAASYEARAFGVRSAMPSTTAMRKCADLVFVPPRFEVYRAVSQQIRAIFADYTPLIE
                     PLSLDEAYLDVTENRRGIATATATAAEIRARILDETGLTASAGISYNKFLAKLASDHR
                     KPNGQFVITPAMGPGFIEGLPVGKFHGVGPVTAAKMNSLGIATGADLRRQSIAFLQRH
                     FGKSGAWYYAIARGEDDRPVVPDRPRKSSGSETTFADDLTDPIAIEAGVQAMADDVWA
                     WCEKAQAFGHTVTVKIKFADFRQATRSRTLNAPVGSQALLLETSIALVRLVYPLTIGI
                     RLVGVTVSNFRDEVPIRQLNLTLSNGPAIEAPFSQSTDKQPADDRYGG"
     gene            1798..2250
                     /locus_tag="VKQ27_16415"
     CDS             1798..2250
                     /locus_tag="VKQ27_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HLY90567.1"
                     /translation="MIASDAFPDDLGRFIAAQEPVIDRVRAELRAGRKSSHWMWFVFP
                     QIAGLGRSETARFYAIRSLEEAAAYLAHPILGQRLVECTRLMNAVKALTAEQILGHVD
                     ALKFHSCMTLFAAVNAGQPDFSDALRRYFGGALDRGTLDLIRPDSAGT"
     gene            complement(2269..2682)
                     /gene="dksA"
                     /locus_tag="VKQ27_16420"
     CDS             complement(2269..2682)
                     /gene="dksA"
                     /locus_tag="VKQ27_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HLY90568.1"
                     /translation="MITLPPDYRPTEDEEFMNPIQVEYFRQKLLRWRTELLKEADGTL
                     ASLSEGGIHEADITDRASVETDRALELRTRDRARKLISKIDQALIRIETGSYGFCEET
                     GEPIGLRRLEARPIATLSIEAQERHERMERVHRDD"
     gene            complement(2858..3496)
                     /locus_tag="VKQ27_16425"
     CDS             complement(2858..3496)
                     /locus_tag="VKQ27_16425"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HLY90569.1"
                     /translation="MSLMRICPIATTGISTARRSGSSTRRTAGLSGKSIVKDFASVCP
                     VMRKCRARPRRTESAASTAPAPGNCCRAEQRGQAMAIESVGWRVTSPVAGRPANRSGS
                     CFSVPADGTAGTATATTGATEPGSLGAVLTLQEMGADTIEDREARKQAQDMLALLAAL
                     QRNLLSGVDNIAALQQLADLAAAAPIAADRRLAATISAILLRVRVELARRHT"
     gene            3393..4511
                     /locus_tag="VKQ27_16430"
     CDS             3393..4511
                     /locus_tag="VKQ27_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HLY90570.1"
                     /translation="MLLPLRPAVRRVLLPLLLAVLIPVVAIGQIRIKDIADVEGIRQN
                     QLVGYGLVVGLNNTGDKLDNAAFTRESLIGMLERLGVNTRDQASKLQTKNVAAVMVTA
                     DLPAFARSGSRIDVAISALGDATNLTGGTLLVTPLLAADGEVYAVAQGAVATGAIAAR
                     GAATSVTRGVPTAARIANGATVEREVQFRLDKSNALRLGLRNPDLTTARRIEQAINKV
                     LGPVAKATDPRTVTLDFASRDPVESLATLEDLRVEPESPAIVVIEEASGTIVMGANVR
                     ISTVAIAQGNLTIRVTETPQVSQPAPFSAGTTTTVPRTQVQIDDQHDRKLGILEGSVS
                     LRDLVASLNALGVGPRDLISILQSIKAAGALQADLIVR"
     gene            4508..4801
                     /locus_tag="VKQ27_16435"
     CDS             4508..4801
                     /locus_tag="VKQ27_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HLY90571.1"
                     /translation="MITAASIPPNASTLPPAQVAKLWQSAQEFEAMAIGQLLQPMFST
                     VDTAHSAFGGREAESAWRPILVDAFGKQMASHGGIGLAVPVFNAMLRAQEAKR"
     gene            4798..5163
                     /locus_tag="VKQ27_16440"
     CDS             4798..5163
                     /locus_tag="VKQ27_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90572.1"
                     /translation="MSADLVEAARNLADVLMEENAALAALDLKQTGALVAGKERAVAA
                     FTAALAGSSLDGAGRPLAQRLRSLADENKTLLERAIAAQGRVMAVFAQAAARQPGYGA
                     ASGRVAACRPVPFALAAQA"
     gene            5237..5944
                     /locus_tag="VKQ27_16445"
     CDS             5237..5944
                     /locus_tag="VKQ27_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HLY90573.1"
                     /translation="MRRAPADVDLCPLYGPLIAPSVAADGCYVIGRIAQSLDGYIATT
                     TGASQWISGPEDIAHTHRLRALFDAVIIGAGTVRADNPQLTTREVEGPSPVRVVLDTE
                     RRLGARYRVFNDGPRTLLCCAADAAGGDRVGGAEVVRLPRAANGLDIGAMRAALADRG
                     LRRIFVEGGGVTVSRFLAAGALDRLHVTIAPMLMGSGIPAFTLPKVVSLADVPRYGWS
                     VYRFGADVLVDIPLTRV"
     gene            5941..6894
                     /locus_tag="VKQ27_16450"
     CDS             5941..6894
                     /locus_tag="VKQ27_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HLY90574.1"
                     /translation="MTDPRAFWTIAPGRGAILPVPSHSSRPDEVWVRTVATGVSRGTE
                     ALVFAGRVPPSQYQAMQAPLMEGEFPFPVKYGYSAVGRTDDGNRVFVLHPHQDRFVAP
                     ASMCIRIPEAVPTNRAVLAANMETALNILWDAGPLPGERMVVIGAGVVGLLAASLLAR
                     IPGARVTVVDIDRNRETVARLLGCNFALPEHAPREQDLAVHASGSEAGLRLALVCAGF
                     EARIVEASWFGDVAPALPLGEAFHSRRLRLIASQVGAVATAMRGRRSHSERLAIALEL
                     LADTRYDALLEGPTRFEDMPGAMPQILAPGGLCHVITYGAE"
     gene            6895..7296
                     /locus_tag="VKQ27_16455"
     CDS             6895..7296
                     /locus_tag="VKQ27_16455"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HLY90575.1"
                     /translation="MFSLTVCDHIMIAHSFRGEEFGPAQKLHGATFAVEAEFRADALD
                     HLQLLVDIGLAKVELRRVLDALDYSNLDDNPIFKEINTTTEYVAFHIHGQLAAACRDG
                     RLGDGGRRVSGLKILLRESPNAWAAYEGPIA"
     gene            7296..>7826
                     /locus_tag="VKQ27_16460"
     CDS             7296..>7826
                     /locus_tag="VKQ27_16460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY90576.1"
                     /translation="MRAALIVPAPFDTVSGGYAYDRRIVAGLREAGHAIRVIELAGQH
                     PLTDDLAREAACAAWDSIADDERPIIDGLALPAFAGLDDALSARSAVGLIHHPTALET
                     GFGANDHASLLALEKRLLPRLARIVTTSETTAEQLAATFGVDRDRIKVIVPGTDDAPR
                     CLGSDGPTCHILAIGTL"
BASE COUNT         1272 a         2372 c         2681 g         1501 t
ORIGIN      
        1 gcaccgttca ccgcgacatc gatgatcggg ttcatcgcat tggccggcat catcgtgcgc
       61 aactcgatcc tgctggtgga cttcgtgcgg catggcggcg gcgcggggcg gacgctgcgc
      121 gagacgttgc tgacggccgg cgcgatccgc ttcaagccga tactgctgac cgcgctgtcg
      181 gcgatgattg gtgcggcgac gatactgacc gatccgatct tccaggggct ggcgatctcg
      241 ctgctgttcg gcctggcgtc ctcgacgctg ctgaccgtgc tggtgattcc cgcgatctat
      301 gtcgtgctgc gcgacgcgga ccgcgtgatg ccgtgatgca tgagctcact caccttcact
      361 ggtgggccgc gcaatattga gcagatcgaa tcggtgccct gcctgggggg acgtgccagc
      421 ggacggccgt gagcgcgccg ccgattcctg ctacatctgt tcgggctatg gtcatgtctc
      481 cgattcggaa gatcatccat atcgacatgg acgccttcta cgcgtcggtc gagcagcgcg
      541 atgatccccg tctgcgcgga cagccggtcg ccgtcggcca cggcagcaag cgaggcgtcg
      601 tcgcagctgc aagttacgaa gcccgcgcct tcggcgtgcg gtcggccatg ccttcgacga
      661 cggcgatgcg gaaatgcgcc gacctcgtct tcgtgccacc acgcttcgag gtgtatcgcg
      721 ctgtctcgca acagattcgc gcgatcttcg cggattatac gccgctgatc gaaccgctgt
      781 cgctcgacga ggcctatctc gacgtgacgg agaaccgccg tggcatagcg actgcgaccg
      841 cgacagccgc cgaaatccgg gcgcggatcc tggatgaaac cggtctcacg gcttcggcag
      901 ggatttccta caacaagttc cttgccaagc tcgcctccga tcatcgcaaa ccgaacgggc
      961 aatttgtcat cacgccggcg atgggacccg ggttcatcga gggattgccg gtggggaaat
     1021 tccatggcgt tggtccggtg actgccgcca agatgaacag cctgggcatt gcgacaggcg
     1081 cggacctgcg gcgccaatca atcgcctttc tgcagcggca tttcgggaag tcgggggcat
     1141 ggtattatgc gatcgctcga ggcgaggatg atcggccagt cgtgccagat cgcccacgta
     1201 agtcctcggg ttcggagacg acatttgccg acgatctgac cgatccgatc gcgatcgagg
     1261 ctggcgtgca ggcgatggca gatgacgttt gggcctggtg cgagaaggct caggctttcg
     1321 gacatacggt gacggtcaag atcaagttcg ccgatttccg tcaggcgacg cgaagtcgaa
     1381 cactgaatgc cccggtgggg tcgcaagcgc ttttgctcga gaccagtatt gcgttggtga
     1441 gactggttta tcccctcacc atcggaattc gtctggtcgg ggtgacggta tccaacttcc
     1501 gcgacgaggt tccgatcagg cagcttaatc tcactttgtc caacggccct gcgattgaag
     1561 cacccttcag tcagtccacg gataagcagc ctgccgatga ccggtatggt ggctagcgaa
     1621 aagtgaatgg tgtggggtgg aaacacagac ttatggcaag tctcgttgac tgtggcccac
     1681 tatgtcatgt cgagcccagc ggcgggttat cggcctcctg acgcaccagt gtgcctgcgg
     1741 agagtgatgg ccggctcaag ggctggccat gacggggctg gagctggcgg agcgggcatg
     1801 attgcatctg acgccttccc agacgatttg ggacgcttta tcgccgcgca ggagccggtg
     1861 atcgaccgcg tgcgggccga gctgcgggcg gggcggaagt ccagtcattg gatgtggttc
     1921 gttttccctc aaattgccgg attgggacgg agcgagacgg cacggttcta tgccatccgc
     1981 tcgcttgagg aagcagcggc gtatcttgcg catccgatac tggggcagcg actggtggaa
     2041 tgtacgcgac tgatgaatgc agtgaaggct ttgacggccg agcagatcct tggacacgtc
     2101 gatgccttga aattccattc ctgcatgacg ctgttcgcgg ccgtgaacgc cggacagccc
     2161 gacttttcgg atgccctgcg ccggtatttc ggcggggcgc tcgaccgagg cacgttggat
     2221 ctcatccggc ctgactccgc cggcacctga cggggcgacg ggtcggtatc agtcgtcgcg
     2281 gtggacgcgc tccatccgct cgtgacgctc ctgagcctcg atggaaagcg tcgcgatcgg
     2341 ccgcgcttcc aggcggcgca gcccgattgg ctcgccagtc tcctcgcaga acccatagga
     2401 cccggtctcg atgcggatga gcgcctggtc gatttttgag atcagcttgc gcgcccggtc
     2461 tcgcgtccgc agttccagcg cgcgatctgt ctccacgctg gcgcggtcag tgatgtccgc
     2521 ttcgtggata ccgccctcag agaggctggc cagtgtcccg tctgcttcct tcagcagctc
     2581 ggtccgccag cgcagcagct tctggcggaa atattcgacc tggatgggat tcatgaactc
     2641 ctcatcctcg gtcggtcggt agtccggcgg tagggtgatc atcatggcgt gtcgaacctg
     2701 ctcttccctc gcgctgcgtc ccgctatgga agccgcggaa gaccccggcc cgtcatggat
     2761 cgcatttgcg cgggcttata gcgacgcgcg atagctccgc caagagtaga tgacgaagct
     2821 tcaacaagtt gacaggcaac gattttggcg gacggtgtca ggtgtgtcgc cgggcaagtt
     2881 ccacacgcac ccgtagtaag atggcggaaa tcgtggcggc cagccgccga tcggcggcta
     2941 ttggcgcggc ggcggccaga tcggccagtt gctgcaacgc agcgatattg tccacaccgc
     3001 tcagcaggtt gcgttgcagg gcggcgagca gtgccagcat gtcctgggcc tgcttgcggg
     3061 cctcccggtc ctcgatcgta tccgcgccca tctcctgcaa tgtgaggacg gcgccaaggg
     3121 agcccggctc agtggcgcca gtggttgcgg tcgcagtccc tgccgtcccg tccgctggga
     3181 cggagaagca ggatccggag cggttcgcgg ggcggcccgc gaccggtgag gtgacccgcc
     3241 aaccgacgct ctcgatcgcc atcgcctggc ccctttgttc cgcccggcaa caattgccgg
     3301 gtgccggtgc tgtggaggcc gcggattccg tgcgccgcgg tctggcacgg cacttgcgca
     3361 tcaccgggca gactgacgcg aaatccttta cgatgctttt accgctgagg cctgccgtgc
     3421 gccgtgtgct gctgccgctt ctcctggccg tgctgatccc cgttgtggcg atcgggcaga
     3481 tccgcatcaa ggacattgcc gatgtcgagg gtatccggca gaatcagctc gtcggctatg
     3541 gcctcgtcgt cggccttaac aacactggcg acaagctgga caacgcggcg ttcacccgcg
     3601 agtcgctcat cggcatgctg gagcggcttg gcgtgaacac ccgcgaccag gcgtccaagc
     3661 tgcaaaccaa gaacgtggcg gcggtgatgg tgaccgcgga cctgccggcg tttgcgcgca
     3721 gcggcagccg gatcgacgtg gcgatttccg ccctgggcga tgcgaccaac ctgacgggcg
     3781 gcacgctgtt ggtcacgccg ctgctcgccg ccgatggcga ggtctatgcc gtggcgcagg
     3841 gggcggtggc gacgggtgcg atcgctgcgc gcggcgcggc cacttcggtg acgcgtggcg
     3901 tgcccaccgc ggcccggatc gccaatggcg ctacggttga gcgcgaggtg cagttccgcc
     3961 tggacaagag caatgccttg cggctcggct tgcgcaaccc ggacctcacg accgcgagac
     4021 ggatcgaaca ggcgatcaac aaggtgctgg gaccggttgc caaggcgacg gatccccgca
     4081 ctgtgacgct cgacttcgcc agccgcgacc cggttgagtc gctggccacg ctggaagacc
     4141 tgcgggttga acctgagagt cccgccattg tggtcatcga ggaagcgagc gggacgatcg
     4201 tgatgggggc caacgtgcga atcagtaccg tggcgatcgc gcagggcaac ctgacgatac
     4261 gggtaaccga gacgccgcag gtcagccagc cggcgccgtt ctctgccggc accaccacga
     4321 cggttccgcg gacccaggtg cagatagacg atcagcatga tcgcaagctg ggtatcctgg
     4381 agggcagcgt gtcgttgcgc gacctggtgg cgagcctgaa tgcactaggc gtcggtcccc
     4441 gtgacctgat cagcattctg cagtcgatca aggccgcggg tgcgctgcag gcggatctga
     4501 tcgtgcgatg atcaccgcgg cttccattcc gccgaatgcc agcacgctgc cgccggcgca
     4561 ggtcgcgaaa ttgtggcagt cggcgcagga atttgaggcg atggccatcg gccagttgct
     4621 gcagccgatg ttcagcaccg tggatacggc gcattcggcg ttcggcgggc gcgaggcgga
     4681 aagcgcatgg cgtccgatcc tggtcgatgc gttcggcaag cagatggcct cgcatggcgg
     4741 catcggtctt gccgttccgg tgttcaacgc catgttgcgc gcacaggagg cgaaacgatg
     4801 agtgcggacc tggttgaggc tgcccggaac ctggccgatg tgctgatgga agagaatgcg
     4861 gcgctcgcag cccttgatct gaagcagaca ggggccctgg tggcgggaaa ggagcgtgcg
     4921 gttgcggcct tcactgctgc acttgcggga tcgtcgctgg atggggcggg aaggcccctg
     4981 gcgcagcgcc tgcgatcatt ggccgacgaa aacaagacac tgctggagcg cgcaattgcc
     5041 gcgcagggcc gggtaatggc ggtattcgca caggctgctg cccggcagcc tggttatggc
     5101 gctgcgagcg gccgagtggc tgcgtgccgg ccagtgccgt ttgcgttggc ggcgcaggcc
     5161 tgagctacca gcgcaagtgg gggtatgtta cagccggcct atggagccac cgccgccgga
     5221 gacatggcgc cgtctgttgc gtcgcgcgcc agcggacgtc gacctttgtc cgctctacgg
     5281 tccgctgatc gcgccatcgg tggcggctga cgggtgctac gtgattggcc ggatcgcgca
     5341 atcgctcgac ggctacattg ccacaacgac gggcgcatcg cagtggatca gcggtccgga
     5401 agatatcgcg cacacgcacc gattgcgtgc cctgttcgac gccgtgatca tcggcgctgg
     5461 cacggtccgg gcggacaatc cgcagctgac gacacgcgag gtcgaagggc cttcacccgt
     5521 gcgggtcgtg ctcgataccg agcgccgact cggtgcacgt taccgagtat tcaacgatgg
     5581 ccctaggacg ctgctgtgct gcgcggcgga tgcggcgggt ggcgatcgtg ttggcggagc
     5641 ggaggtggtg cggctgccgc gcgccgccaa cggtctcgat atcggggcga tgcgggcggc
     5701 tcttgcggat cggggcctgc gccggatctt cgtcgagggt ggcggggtta cggtgtcgcg
     5761 ctttctcgcc gcgggggcgc tggatcggct gcacgtgacg atcgcgccga tgctgatggg
     5821 ctctggcatt cctgcgttca cgttaccgaa agtcgtgtcg cttgccgacg tgcctcgcta
     5881 cggctggtcg gtgtatcgct tcggtgctga cgtgctggtc gatattccgc tgacgcgcgt
     5941 atgaccgacc cgcgcgcgtt ctggacaata gcaccagggc gtggcgcgat cttgcctgtt
     6001 ccgtcgcatt catctcgtcc ggacgaggtg tgggttcgta ctgtggcgac cggcgtttca
     6061 cgcggcaccg aggcattggt gttcgcgggc cgcgttcccc ccagccagta tcaggcgatg
     6121 caagcgccgc tgatggaggg agaattcccg ttcccggtca aatacggcta cagcgctgtc
     6181 ggccgcacgg atgatggcaa ccgtgtcttc gtgctgcatc cacaccagga ccggtttgtt
     6241 gcccccgcaa gcatgtgcat tcgtatccct gaagccgtgc cgacgaaccg cgcggtactc
     6301 gcagccaaca tggagaccgc actcaatatc ctgtgggatg ccgggccgct tcccggtgag
     6361 cggatggtgg tcatcggcgc aggggttgtc ggactgctgg cggcgtcgtt gctggcgcgc
     6421 attcccggcg cgcgcgtcac agtggtcgat atcgatcgga accgtgaaac tgtcgcgcgg
     6481 ttgctcggct gcaacttcgc gctgccggag catgcgccac gcgaacagga tctggcggtg
     6541 catgcttcag gaagcgaggc aggcttgcgg cttgcgctgg tttgcgcggg tttcgaagcg
     6601 cgtatcgtcg aagcgagctg gttcggcgat gttgcgccgg ccttgccgct tggcgaagcg
     6661 ttccattcgc ggcggctgcg gctgatcgca tcgcaagtcg gtgcggtcgc aactgcgatg
     6721 cgcggacggc gcagccattc ggagcgtctg gcgatcgctc tcgaactgct ggccgatacg
     6781 cgatacgacg cattgctgga aggtccgacg cggttcgagg atatgccggg agcaatgccg
     6841 caaatcctcg cgccgggtgg actgtgccat gtcatcacgt atggggcgga gtgaatgttc
     6901 agcctgaccg tctgcgatca catcatgatt gcccacagct ttcgcggcga ggagttcggt
     6961 ccggcgcaga agctgcacgg cgcgaccttc gcggtggaag cagagttccg cgcggacgcg
     7021 ctcgatcatc tgcagctgct ggtggatatc ggccttgcca aggtcgagct gcggcgcgtg
     7081 ctcgatgcgc tcgactacag caatctggac gacaatccga tcttcaagga aatcaacaca
     7141 acgaccgaat atgttgcgtt ccatatccac ggtcagcttg ctgcggcgtg ccgcgatggc
     7201 cggctgggcg atggtggccg gcgggtgagt ggcctgaaga tcctgctgcg cgagagcccg
     7261 aatgcctggg ctgcctatga ggggccaata gcctgatgcg ggccgcgctg attgtcccgg
     7321 cgccgttcga taccgtttcg ggtggctatg cctatgatcg ccgcatcgtc gctggtctgc
     7381 gtgaagccgg gcacgcgatc cgggtcattg aactggcagg tcagcacccg ctgaccgatg
     7441 atctggcgcg cgaggccgca tgtgctgcgt gggacagcat tgccgatgat gagcgcccga
     7501 tcatcgatgg gctcgcgctg cctgcatttg cgggtctgga tgatgcgttg tccgcgcgca
     7561 gcgcggttgg gctgatccat catccgaccg cgctggaaac aggcttcggc gccaatgatc
     7621 acgcgtcgct gcttgcgttg gagaagcggc tgctgccgcg gcttgcgcgc atcgtgacca
     7681 ccagtgagac cacggccgag cagcttgccg ccacattcgg ggtcgatcgg gaccgcatca
     7741 aggtcatcgt acctggtacc gatgatgcgc cgcgctgtct gggatcagac gggccaacgt
     7801 gccacatcct ggccatcggc acgctg
//
LOCUS       DATGEM010000539        11043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1059809, whole genome shotgun sequence.
ACCESSION   DATGEM010000539 DATGEM010000000
VERSION     DATGEM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059809"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VKQ27_16465"
     CDS             <1..231
                     /locus_tag="VKQ27_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90577.1"
                     /translation="VILLDVFMPELDGIDVLHEILLAGIPTRVILTTGGDEELLSVAR
                     EVVRFHGVEEPVMLPKPFRRAELLAALTRAVG"
     gene            complement(244..378)
                     /locus_tag="VKQ27_16470"
     CDS             complement(244..378)
                     /locus_tag="VKQ27_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HLY90578.1"
                     /translation="MKAATVAIAALLSLLMLAACGKKGPPSPPGPANQITYPRVYPTH
                     "
     gene            complement(375..1784)
                     /gene="argH"
                     /locus_tag="VKQ27_16475"
     CDS             complement(375..1784)
                     /gene="argH"
                     /locus_tag="VKQ27_16475"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLY90579.1"
                     /translation="MSETPDRRNAAANPQWGGRFAAGPSMVMQEINASIGFDQKLWRQ
                     DIRGSLAHAAMLAQVGVITAEDERAIGTGLAAIAQEIEMGRFTFEAALEDIHMNIEAR
                     LTERIGDAGRRLHTARSRNDQVATDFRLWVRDAIDGLTEQITDVMRALADRAAAHAGD
                     PMPGFTHLQTAQPVTFGHHLLAYVEMLDRDRGRLADARRRLNECPLGCAALAGTSFPI
                     DRHMTAKALGFERPTANSLDAVSDRDFALEFLGAAAISATHLSRFAEEIVIWCSAPWR
                     FIRLSDAFTTGSSIMPQKRNPDAAELARAKTGRITGALVGLLTVMKGLPLAYAKDMQE
                     DKEPVFDAAEAWALSLAAIAGMVRDMAPDTARMREFAGSGYATATDLADWLVRVLKLP
                     FRDAHHTTGRLVAIAEAKGVDLANVTLPEMQSVEPRITADVFSILTVDASVASRTSFG
                     GTAPTNVAREAARWKAGLA"
     gene            1797..2342
                     /locus_tag="VKQ27_16480"
     CDS             1797..2342
                     /locus_tag="VKQ27_16480"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLY90580.1"
                     /translation="MMLRRTIFAAAGTLAGALIARKPRAGELDNLTKVLKPLDPPVPA
                     PDLPFLAADGSEHRLRDFLGHGMVVNMWATWCAPCVEELPSLAALAKRLAPDDIAVLP
                     LSSDRGGAKTVQTYFQAHGISGLPVLLDPHSTAAHAWNVHGIPTSFVIDKKGRQVAQL
                     EGSADWSTPSAAQLVRKLVGV"
     gene            complement(2391..2780)
                     /locus_tag="VKQ27_16485"
     CDS             complement(2391..2780)
                     /locus_tag="VKQ27_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90581.1"
                     /translation="MANHYVLHGDGIQIDYTIGGNPGFTALSFTEGGTTKNFTASQVT
                     TDVTGLGTLVSVALVQSVDTGGTRFGFFLPEVQVVLGQQVPVATIGIFEMFSGPDSVP
                     HRLTTWQCVHLHGMAEEVEVALVKAPA"
     gene            complement(2930..4114)
                     /locus_tag="VKQ27_16490"
     CDS             complement(2930..4114)
                     /locus_tag="VKQ27_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLY90582.1"
                     /translation="MTVRAGREFLAIPGPTTVPDEVLRAMHRPAVDIYSGPLLALTDS
                     LLRDMPRVFNTTGRAYIYISNGHGAWEAALTNVLSRGDHILVLESGRFAVGWGNAAAR
                     MGVEVEILKGDMRRAVRPAEVEARLKADKGGTIKAVLVAQIDTASGVVNDIAAIGQAI
                     RSAGHDALLMVDAVASLGCMPFEMDEWGVDVAMSGSQKGMMAPPGLGFVAANDRAREV
                     HKSADLRTPYWDWTGREGEEHYQKYAGTPPEHLLYGLRQALDLMFEEGLANVFRRHAL
                     LAEAVRRAVGVWSEGQVLSFNVIEPGERSNTVTPVLTNGHNPDVLGDYCREKCGVILG
                     HGIGDLSGKAFRIAHMGHVNAPMILGTLGVIEMALAALNVPHGKGGAQAAIDWLGEQV
                     AA"
     gene            complement(4111..4887)
                     /locus_tag="VKQ27_16495"
     CDS             complement(4111..4887)
                     /locus_tag="VKQ27_16495"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HLY90583.1"
                     /translation="MVPIFHRLIRPAHKAPFNPIYNPARVYMPEDTTIVTPNSDTPLS
                     PWTEVGKRQYISFQFTDTYTANFAYAGTRTTGNGPSCFMVAGPGWSGEKPPGITRLFR
                     CETEFAAVLIRTQLFDSADIDNVKKIQAGYCAEPLSTFLNKPSPPEVNWPKINKQMAE
                     KGPFGYPNFVLQFWPPTGSAAVEAPLRAPGCELLQSSGTSCAITRVSTTSARRERPRV
                     AGSSPAVTIERRAGARIHGERPGQTGHDDKKDWANPRNHA"
     gene            complement(5093..6529)
                     /locus_tag="VKQ27_16500"
     CDS             complement(5093..6529)
                     /locus_tag="VKQ27_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLY90584.1"
                     /translation="MFTPDQNRQQAVFDRLRQGILSGTLPAGARLPPTRALADELGVA
                     RQTVVLAYERLASEGYVRARTGSGTFVSPDLPDAAPAPATPPPTAASSLSRRGQQIAV
                     VPVSAHPRQPTLGTLLAGGIPAPDLFPREAWARCAAKVMKTFPADLTGYPSAQGLPEL
                     RTQIAAHLAATRGLLADPDNIVVTAGTQQALRIAADLLLDPDDTAWVEDPGYIAGRGA
                     LLAAGATPVPVISDIEGLDVAAGIQRAPDARLALVAPSHATPLGGALPIARRLALLDW
                     AARANAWILEDDCDSEFRWSGHPLPPLATLDRAGRVIYCGTFSKTLAPALRLGFAVVP
                     APLVPAFVRACALSGRGIDTFNQGTLAEFMRQGLLAPHIRRMRTEYSRRRDALLAALA
                     RHAPSIRPIDAPGGLHMVGCLSAGTDEAGAVVACRARGLAVSPLGTYYAGPPRMTGLI
                     MGFAGTPAALAADTAKRLEAALRGGNGK"
     gene            complement(6673..6876)
                     /gene="rpsU"
                     /locus_tag="VKQ27_16505"
     CDS             complement(6673..6876)
                     /gene="rpsU"
                     /locus_tag="VKQ27_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLY90585.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRRHYEKPSERRA
                     REAAEAVRRARKLERKRIEREGF"
     gene            6962..7501
                     /gene="def"
                     /locus_tag="VKQ27_16510"
     CDS             6962..7501
                     /gene="def"
                     /locus_tag="VKQ27_16510"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLY90586.1"
                     /translation="MAILKIARMGHPVLLQKCEPVKDPGAPEIRRLVADMMETMQDAA
                     GAGLAAPQVYVPLRLFVFRVAPGRTEQDPDDVPLGNSVLINPEVELMGEERVLGWEGC
                     LSIPGLRAAVPRAGRVRYSGMDCDGLPVERVVSGFHARVVQHENDHLDGILYPMRMRD
                     FSLFGFNEELSRAAATALQ"
     gene            7498..8109
                     /locus_tag="VKQ27_16515"
     CDS             7498..8109
                     /locus_tag="VKQ27_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HLY90587.1"
                     /translation="MTPPERSAERDAAIEAMLPHVPFDGWTLGAVRRGLASIGAEPED
                     AALLFPGGVADMIEAFCDWADRRMEEGAAALDPGLRLHERVRAVIALRLEQNRPFKEA
                     IRRALAVLAMPGHARLAAGCTARTVDATWHAAGDRSADFSWYTKRAILAGVYGATLLY
                     WLRDSSDDDAATLAFLDRRLAGVGQITSVRRRVEGAVEKLKAA"
     gene            8204..9016
                     /locus_tag="VKQ27_16520"
     CDS             8204..9016
                     /locus_tag="VKQ27_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLY90588.1"
                     /translation="MTDRYTRYTGLFFDRPHPRVLRITMGDRPERLNVVDAVMHRELG
                     DIWRDVDADPEVNAVIITGAGRAFSAGGDFAMIEEMMADFDTRARIWKEARDLVYNII
                     NCGKPVVSAMRGPAVGAGLVCGLLADVSIASKDARIIDGHTRLGVAAGDHSAIVWPLL
                     CGLAKAKYYLLLCDAVSGAEAERIGLVSLAVDNAELDAKALEVATRLAEGAQSAIRWT
                     KYALNNWLRLAGPSFDASLAMEFMGFSGPEAQEGLASHKEKRRPSFPPGSPV"
     gene            complement(9030..9290)
                     /locus_tag="VKQ27_16525"
     CDS             complement(9030..9290)
                     /locus_tag="VKQ27_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90589.1"
                     /translation="MSWSLASVAATANCTASLGSMMNVGGYIGGAFASTITGFIVQRT
                     GWFEQALLLGAFIALVGAASYILIVRQAPIQVTELEGILATA"
     gene            complement(9705..10463)
                     /locus_tag="VKQ27_16530"
     CDS             complement(9705..10463)
                     /locus_tag="VKQ27_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HLY90590.1"
                     /translation="MRIEYLYRYPVKGLTAEALEAAEVEQGGCIPWDRAFALAQGGSG
                     FDPQHPAWLQKQNFMCLMKNARIASLFSFFDPRSGMLAIRAPDGSSVVENALTPEGRD
                     RIGAFLVAYIGEEARGAPSFHHVPGHSFCDQRHKVVSLINLASLRDYEGKVGARRHRR
                     RFRSNIWFSGAAAWSERDWVGQQIQMGGAVLRVTKGITRCPATQVNPETASRDADPVE
                     ELRGLYGHVELGIHAQVIEGGKFAVGDAIELLGQ"
     gene            10583..>11043
                     /locus_tag="VKQ27_16535"
     CDS             10583..>11043
                     /locus_tag="VKQ27_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HLY90591.1"
                     /translation="MDIEKFTERARGFLQSAQTIAIREFHQQITPQHLLKALLDDEEG
                     AAAGLIGTAGGNPKAALSAVEAELARQPKVQGSGAGQPQITPDLVRLLDAAQQAATKA
                     GDEYVAQDRLLVAIAASDTPAARALRQAGATPQALEKAVNDIRKGRKVTSA"
BASE COUNT         1909 a         3361 c         3850 g         1923 t
ORIGIN      
        1 gtgatcctgc tggacgtatt catgccggag ctcgacggga tcgacgtcct gcacgagatc
       61 ctgctcgccg gcatcccgac ccgcgtcatt ttgacaacgg gaggtgacga ggagctgctc
      121 tcagtcgcgc gggaggtagt acgatttcat ggtgtcgagg aaccagtcat gctgccaaag
      181 ccgttccgcc gcgcggagct gctggcggcg ctgacccgcg cggtgggtta atcgccatcc
      241 cggtcagtgg gtcgggtaga cccgtggata cgtgatctga ttggccggtc cgggcggcga
      301 aggcggacct ttcttgccgc aggccgcgag catcagcagc gagagcagag cagcgatcgc
      361 aactgtggca gccttcatgc cagcccggcc ttccagcgag cagcctctcg agccacattg
      421 gttggtgcgg tgcccccgaa actggtgcgg gaggcgacgg acgcatccac tgtcagaata
      481 gagaacacat cggcagtgat gcgtggttcg acggattgca tctcgggcag cgtcacattg
      541 gccagatcga cgccctttgc ctcggcaatc gccaccaagc gaccagtcgt atggtgcgcg
      601 tcgcggaatg gcagcttcag tacgcgcacc agccagtccg cgagatcggt cgcggtggca
      661 tagccggagc ccgcgaactc gcgcatcctg gcagtgtcgg gcgccatgtc gcgcaccatg
      721 ccggcgatgg cggcgagcga caaagcccag gcttcagcgg catcgaacac cggttccttg
      781 tcctcttgca tgtccttggc gtacgcgagc ggcagccctt tcatcactgt cagcaggccg
      841 acgagcgcgc cagtgatgcg gccggtcttg gcacgcgcca gttcggcggc gtccgggttg
      901 cgtttctgcg gcatgatcga gctgccggta gtgaaggcgt cgctcaggcg aatgaagcgc
      961 cagggagccg aacaccagat cacgatctcc tcggcgaagc gcgacagatg tgtcgcggag
     1021 atcgcggcgg cgccgagaaa ctccagcgca aagtcgcggt ccgacaccgc atccagcgaa
     1081 ttggctgtcg gccgttcgaa gccgagggcc ttggctgtca tgtggcgatc gatcgggaac
     1141 gaagtgccgg cgagcgcggc gcaacccagc ggacattcat tcagacggcg ccgcgcatcg
     1201 gcaaggcgcc cgcggtcgcg atccagcatt tcgacatacg ccagcagatg atgcccgaat
     1261 gtaaccggct gcgcggtttg cagatgggtg aagcctggca tcggatcgcc cgcatgcgcc
     1321 gccgcccgat ctgccagcgc acgcatcacg tccgtgattt gctcggtcag gccatcgatc
     1381 gcgtcgcgca cccataggcg gaaatcggtc gccacctggt cattgcggct gcgcgccgta
     1441 tgcaggcggc gcccagcgtc gccgatgcgc tcggtaaggc gggcctcgat gttcatgtgg
     1501 atgtcttcga gcgcggcctc gaacgtgaaa cgccccatct cgatctcttg cgcgatcgcg
     1561 gctagaccgg tgccaatggc tcgctcatct tcggccgtga tgaccccaac ttgcgccagc
     1621 atggccgcat gcgcgagcga gccgcgaatg tcctgccgcc acagcttttg gtcgaagccg
     1681 atggaggcat tgatctcctg catcaccatg gacggtcccg cagcgaatcg accgccccat
     1741 tgcgggttcg cggctgcgtt gcgcctgtct ggtgtttcgc tcaaaggagg gcttccatga
     1801 tgctgcgccg gaccattttc gcggcggccg ggaccctagc cggcgccctc atcgcgcgca
     1861 aacctcgcgc cggggaattg gataacttga cgaaggtgct gaagccgctg gatcccccgg
     1921 tgccggcgcc cgaccttccg ttcctggcag ccgacggcag cgagcatcgg ctgcgcgatt
     1981 tccttggcca cggcatggtg gtgaacatgt gggcgacgtg gtgtgcgccg tgcgtggaag
     2041 aactgccgtc actggccgcg ttggcgaagc ggctggcacc ggatgatatc gcggtgctgc
     2101 cactgtcgtc cgatcgcggc ggagcgaaga cggtgcaaac ttattttcag gcccacggga
     2161 tcagcggcct gccggtgctg ttggacccgc acagcaccgc tgcacacgcg tggaacgtgc
     2221 acggcattcc gaccagcttc gtcatcgata agaagggacg ccaggtggcg cagctggagg
     2281 ggtccgccga ttggtcaacg ccatctgcgg cacagctggt acggaaactg gtgggtgtgt
     2341 agagcgcaag cgaccggcgt gacccggccg cttgctttcg tggactgatg tcaggctggc
     2401 gctttcacca gtgctacctc cacttcctcc gccatgccgt gcaggtgcac gcactgccag
     2461 gtcgtcagcc tgtgcggcac gctgtccgga ccgctgaaca tttcgaatat gccgatagtt
     2521 gccaccggta cctgctggcc gagtacgacc tgcacctcgg gcaggaagaa gccgaacctg
     2581 gtgccgccag tgtccactga ttgcaccaac gccacagaca caagggtccc aaggcccgtg
     2641 acgtcagtgg tgacctgcga cgcagtgaaa ttcttggtgg tgccaccttc ggtaaacgaa
     2701 agtgcagtga agcctgggtt gccgccgatg gtgtagtcga tttggatccc atcgccgtga
     2761 agtacgtagt gatttgccat gtaatcctcc gctcgttctg ttgccggcgg gaagccgcgg
     2821 ccggtgacgc tcacgttatg gcgacgccga cgaacgccgt gtgaggcggc tcacacgggg
     2881 caattgtggc gcatctgatc gtgattatgt gaacgtaacg taggcttggc tacgccgcca
     2941 cctgctcgcc aagccaatcg atggcggcct gcgcgccgcc cttgccatgc ggaacattca
     3001 gtgccgccag cgccatctcg atgacgccga gagtcccgag gatcatcggt gcattcacat
     3061 gccccatatg ggcgatgcgg aacgccttgc ccgacagatc gccaatgccg tggcccagga
     3121 tgacaccgca tttttcgcgg caataatcac ccaggacatc cgggttgtgg ccgttcgtca
     3181 gcacgggggt taccgtgttg ctacgctcgc ctggttcgat gacgttgaag ctcagcacct
     3241 gaccctccga ccagacaccg acggcacgcc ggactgcctc ggccaacaac gcgtggcggc
     3301 ggaagacatt ggcgaggcct tcctcgaaca tcaggtcgag cgcttggcgc agaccataga
     3361 gcaggtgttc gggcggagtg ccggcgtact tctgatagtg ttcctcgccc tcccggccag
     3421 tccagtccca gtacggtgtg cgcagatcgg ccgacttgtg cacctcgcgg gcgcggtcat
     3481 tggcggcgac gaagccgaga ccaggtggcg ccatcatccc cttctgtgag ccggacatag
     3541 cgacatccac gccccattcg tccatctcga acggcatgca gcccagcgag gcgacggcat
     3601 ccaccatcag cagcgcatcg tggccggcgg aacggatcgc ctggccgatg gcggcaatat
     3661 cgttcaccac gcccgacgca gtatcgatct gcgccaccag caccgcctta atggtgccgc
     3721 ccttgtcggc cttcaggcgg gcttccactt cggccggacg gacggcacgg cgcatgtcgc
     3781 ccttcaggat ctccacttcc acgcccatac gcgctgcggc gttgccccag cctacagcga
     3841 agcggccgct ctccagcacg aggatgtggt cgccgcgaga aagcacgttg gtcagtgcgg
     3901 cttcccaggc gccatggccg ttcgagatgt agatgtaggc gcggcctgtc gtgttgaaca
     3961 cgcggggcat gtcgcgcagc aggctgtcag tgagggcaag caacgggccg gagtagatgt
     4021 ccacggccgg gcggtgcatc gcgcggagca cttcgtcggg aacggtggtg gggccaggaa
     4081 tggcaaggaa ctcgcggcct gcgcgtacgg tcatgcatgg ttccgggggt tagcccagtc
     4141 cttcttatcg tcatggccgg tttgacccgg ccgttctcca tggatccggg cgccagcccg
     4201 gcgctctatc gtcacggctg ggcttgaccc ggccaccctc gggcgttctc tccgcgccga
     4261 agtggtacta acgcgggtga tagcacagga cgttccgctt gactgcaaga gctcgcaacc
     4321 gggcgcgcgc aatggcgcct cgacagcggc gcttcccgtt ggcggccaga actgcagcac
     4381 gaagttcgga tagccaaagg gacccttctc ggccatctgc ttgttgatct tcggccagtt
     4441 gacctccgga ggcgacggct tgttcaggaa tgtggaaagc ggctcggcgc aatacccagc
     4501 ctgaatcttc ttcacgttgt cgatgtcggc cgaatcgaac agttgggtgc gaatgagcac
     4561 cgccgcgaac tcagtttcgc accggaacag acgggtaatt cccggcggct tctcgccact
     4621 ccagcccgga cccgcgacca tgaagcagct gggaccgttg cctgtggttc gggtgccggc
     4681 atacgcgaag ttggcggtgt acgtgtcggt aaactgaaac gagatatact gtcttttccc
     4741 gacctcggtc catggcgata acggcgtgtc gctgttcggc gtcacaattg tcgtatcttc
     4801 aggcatatag acgcgggccg gattgtagat cgggttgaac ggcgccttgt gcgcaggtct
     4861 gattagtcga tgaaatattg gtaccaaaca ccgtagttca tcaccatcgg aaagccgtag
     4921 ctgaaatctt gctcggcgat aatgacattt ggcccggcgc tacgtccgct ccgtagcctc
     4981 tggtagtcat gctggcggcc gcaattccgc ccgataccag agaagccagg gagcgacgcg
     5041 tgatcacatt ggtttcccct cgcacatgtt gcttctgtcc ggatcgtggg ggttacttgc
     5101 cattaccccc acgcaatgcg gcttccagcc tcttcgccgt atccgcagcc agcgctgccg
     5161 gcgttccagc aaaccccatg atcagccccg tcatgcgcgg cgggccagcg tagtaggtgc
     5221 cgagcggcga caccgccaag ccccgcgcgc ggcatgcgac aacggcaccc gcctcatccg
     5281 tgccggctga tagacagccc accatgtgta ggccgccagg cgcgtcgatc gggcggatcg
     5341 agggcgcgtg gcgggccagc gccgccagca gggcgtcgcg gcggcgggaa tactcggtgc
     5401 gcatgcggcg gatgtgtggg gccagcaggc cctggcgcat gaactcggcc agcgtcccct
     5461 ggttgaaagt atcgatgccg cggccggaca gcgcgcaggc gcggacgaag gcgggaacca
     5521 ggggcgcggg gaccacggcg aagccaaggc gcagcgcagg ggcaagtgtc ttgctgaacg
     5581 tgccgcagta gatcacacgc ccggcacggt cgagcgtggc gagcggcggc agcggatggc
     5641 cgctccagcg gaactccgag tcgcagtcat cttccaggat ccaggcgttg gcacgggcgg
     5701 cccagtccag cagggcgagc cgccgggcga tgggcagggc accgccgagg ggcgtggcat
     5761 gggacggcgc gaccagggcc aggcgcgcgt ccggcgcgcg ctggatgccg gcggcgacat
     5821 ccaggccctc gatgtcgctg atgacgggga ccggggtggc gccggcggcg agcagcgcgc
     5881 cgcggccggc gatgtagccg gggtcctcga cccaggcggt gtcgtcgggg tccagcagca
     5941 agtcggcagc gatacgcaga gcctgctggg tgcccgcggt gaccacgatg ttgtccggat
     6001 cggcgagcag gccgcgggta gcggcaaggt gggcggcgat ctgggtgcgc aactcgggca
     6061 gaccctgggc cgaggggtag ccagtgaggt cggccgggaa agtcttcatc accttggcgg
     6121 cgcagcgggc ccaggcttcg cgcgggaaca ggtcgggtgc cgggatgccg ccggccagca
     6181 gtgtgccgag cgttggctga cgagggtggg cggagacggg gacgaccgcg atctgctggc
     6241 cgcggcggga gagggaactg gcggcagtgg gcggtggcgt agccggtgcc ggggcggcgt
     6301 caggaaggtc gggggagacg aaggtaccgc tgcccgtgcg agcgcggaca tagccctcag
     6361 acgccaagcg ctcgtaggcc aggacaacgg tctgacgggc cacgcccagt tcgtcggcaa
     6421 gggcgcgggt cggcgggagg cgggcgccgg ctggcagtgt gcctgaaagg atgccctggc
     6481 ggagcctgtc gaataccgcc tgctggcgat tttggtctgg tgtaaacatg gcggcagcgc
     6541 cctggacgtt ggtccaattg gaccgtaacg cagagcaact attggggcaa gtcagtagac
     6601 cccccttccc taccccccgg atcaagcccg ggggcatggt cccctgctcc gcccagagag
     6661 gggatgtctt gcttagaagc cttcgcgctc gatgcgcttg cgctccagct tgcgggcacg
     6721 gcggacggct tcggcagcct cgcgggcccg gcgctccgac ggcttctcgt agtgccgtcg
     6781 cagcttcatc tcgcggaaga ttccttcccg ttgcatcttc ttcttgagcg ctttcagcgc
     6841 ctgatcgaca ttgttgtcgc gtacgagcac ttgcacttgg ccgccttctc cttctctgct
     6901 gataagcgcc cccgccaatc ggggacgcgc cctatatcac gtggcaacgc cggagcaata
     6961 gatggcaata ctgaagatag cgcgcatggg ccatcccgtg ttgttgcaaa aatgtgagcc
     7021 ggttaaggat cccggcgccc cggagatccg gcggctggtg gccgatatga tggagacgat
     7081 gcaggatgcc gccggggcgg gcctggcagc gccgcaggtg tatgtgccgc tacggctgtt
     7141 cgtgttccgg gtggcgccgg ggcggaccga gcaggatccg gacgacgtgc cgctcggcaa
     7201 ttcggtgctg atcaatcccg aggtggagct gatgggcgag gaacgcgtcc tgggatggga
     7261 gggatgcctg tcgatccccg ggctgcgcgc ggcggtgcct cgtgcgggac gggtacggta
     7321 tagcgggatg gattgcgatg gcctgccggt ggagcgggtg gtgtcggggt tccacgcgcg
     7381 cgtcgtgcag catgagaacg accatctgga cgggatcctg tatcctatgc ggatgcggga
     7441 tttctcattg ttcgggttca atgaggagct gtcgcgcgca gcagcgacgg cgctgcaatg
     7501 accccgccgg agcgaagcgc cgagcgggac gcggcgatcg aggcgatgct gccccacgtg
     7561 ccgttcgatg ggtggacgct gggggcggtg cgtcgcggct tggcgtcgat cggggccgag
     7621 ccggaggatg cggcgctgct gttccctggc ggcgtggcgg acatgatcga ggcattctgc
     7681 gattgggcgg atcggcggat ggaggaaggc gcggcagcgc tcgacccagg actccggctg
     7741 catgagcgcg ttcgggcagt gatcgcgctg cggctggagc agaaccggcc gttcaaggag
     7801 gcgatcaggc gtgcgctggc ggtgctggct atgcccgggc atgcgcggct ggcggcggga
     7861 tgtacggctc ggactgtgga cgcgacctgg catgcagcag gcgatcggtc ggcggacttc
     7921 tcctggtaca ccaagcgggc gatcctggcg ggcgtgtatg gcgcgacgtt gctgtactgg
     7981 ctgcgggaca gcagcgacga tgatgcggcg acgctggcgt ttctggatcg gcggctggct
     8041 ggggttgggc agattacgtc ggtgcggcgg cgggtcgaag gggcggttga aaagctcaag
     8101 gccgcatgaa ctgatcggcg ccaggacaat gcgacccgtc acgcaccccg aaatcgcttc
     8161 gcccttttgc gccttaggat gactgggtaa ccggagtgct accatgactg atcgctacac
     8221 acgctacacg ggtctgttct tcgaccgtcc ccacccgcgt gtgctgcgca tcaccatggg
     8281 ggatcggccg gagcgactga atgttgtcga tgcggtgatg catcgcgaac tcggggatat
     8341 ctggcgcgat gtcgatgccg atccggaggt gaacgcggtg atcatcaccg gcgcggggcg
     8401 ggcattctcg gcgggtggcg acttcgcgat gatcgaggag atgatggccg acttcgatac
     8461 ccgggcccgg atttggaagg aggcgcgcga cctcgtctac aacatcatca actgcggcaa
     8521 gccagtggtg agcgcgatgc gtggaccggc ggtaggggct gggctggtgt gcgggctgct
     8581 cgcggatgtg tcgatcgcga gcaaggatgc gcggatcatc gacggccaca cgcgattggg
     8641 ggtcgcagcg ggggaccatt cggcgatcgt gtggccgctg ctgtgcgggc tggcgaaggc
     8701 gaaatactac ctgctgctgt gcgacgccgt gtcaggggca gaggcggaac ggatcgggct
     8761 ggtctccctg gcggtggaca atgcggagct tgatgcgaag gcgctggaag tggcgacgcg
     8821 gctggcggag ggagcacaga gcgccatccg ctggacgaag tacgcgctga acaactggct
     8881 gcggctggcg gggccgtcgt tcgatgcgtc tttggcaatg gagtttatgg ggttcagcgg
     8941 gccggaggcg caggaaggcc tcgcgtcgca caaggagaag cggcggccgt cattcccgcc
     9001 gggttcgccg gtgtgacggc cgctgggact caagctgtgg cgaggatgcc ctccaactcg
     9061 gtcacctgaa tcggcgcctg gcggacgatc aggatataag aggccgcgcc gaccagggcg
     9121 atgaaggcgc caagcagcag ggcctgctcg aaccagccgg tgcgttgcac aatgaagcct
     9181 gtgatcgtcg aggcgaatgc accaccgata tagccaccga cgttcatcat gctgccaagc
     9241 gaggcggtgc agtttgcggt ggcagctacg ctcgccagtg accaggacat gccgctggca
     9301 cctccgttac ggaacaccgc ggccgagatg caggcgacgg cgatgaaatt gctgccggtt
     9361 tcagcggcaa tgttagatga agacggccat gccgagcagg ccgcggggga ggagtggccg
     9421 ctgacggttt ggggactccg tcagcggccg ggaagtgatg tcagatggcg caaaggccaa
     9481 gctaggcgaa gatcagtcca gccgcgatcg tcatagctac aaaatagtag gcaaggcccg
     9541 gcgtgctggc ccatctggaa gctcttggcc gtgtcacacc gtcattctca aattgcgcga
     9601 attccaactg aaatgtgctc gcttcaaatg agtctgtgcg gcgttactta cctcgcgaaa
     9661 ccgtgatcga gcaattttat tctcacgaaa tcgcgatcaa cttttcattg tcctagcagt
     9721 tcgatcgcgt cgccgacggc aaatttgccg ccctcgatga cctgggcgtg gatgccgagt
     9781 tcgacgtggc cgtaaagccc acgcagttct tcgacggggt cggcgtcgcg actggccgtt
     9841 tccgggttta cctgggttgc cgggcagcgg gtgatgccct tcgtcacccg cagcacggca
     9901 ccgcccatct ggatctgctg gccgacccag tcgcgctcgc tccaggcagc ggcgccgctg
     9961 aaccagatgt tcgagcggaa gcggcggcga tggcggcggg cgccgacctt gccttcgtag
    10021 tcgcgcaggc tggcgaggtt gatcaggctg accaccttgt gccgctggtc gcagaagctg
    10081 tggccaggga catggtggaa gctgggggcg ccgcgagcct cctcgcctat ataggccacc
    10141 aggaaggcgc cgatacggtc tcgcccttct ggggtcaagg cgttctcgac caccgaggag
    10201 ccatcggggg cgcgaatggc cagcataccg gaacgcgggt cgaagaacga gaagagcgag
    10261 gcgatccggg cgttcttcat aaggcacatg aagttctgct tctgaagcca ggcagggtgc
    10321 tgagggtcga agccggagcc gccctgggcc agggcaaagg cacggtccca gggaatgcag
    10381 ccgccctgtt cgacctccgc ggcttccagc gcctcggcgg tcagcccctt caccgggtaa
    10441 cggtagagat attcgatgcg cattgctcaa gccctcttga gggacagttt gccttcaccc
    10501 acttggattg ccaaagggtc tcgtttccgt tgcctgatca ggggtgggga cagggcctgt
    10561 cgcctatgaa gggacacaac gaatggacat cgagaaattc accgaaaggg cgcgtggatt
    10621 cctccagtct gcccagacca tcgcaatccg cgaattccac cagcagatca cgccgcagca
    10681 tttgctgaag gcgctgctgg atgatgagga aggcgctgcg gcagggctga tcggcacggc
    10741 cggaggcaac ccaaaggcgg ctctgagcgc cgtcgaggcc gaactggcac ggcagccgaa
    10801 ggtacagggc tctggcgcgg gccagccgca gattacgccc gacctggtgc ggctgctgga
    10861 tgccgcgcag caggccgcga cgaaggcggg cgacgagtat gtcgcgcagg accggctgct
    10921 ggtggcgatc gctgcctcgg acacgccggc ggcgcgcgcg ctgcgccagg ctggcgccac
    10981 gccgcaggcg ctggagaagg cggtcaacga catccgcaag gggcgcaagg tcaccagcgc
    11041 caa
//
LOCUS       DATGEM010000540         4792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_831963, whole genome shotgun sequence.
ACCESSION   DATGEM010000540 DATGEM010000000
VERSION     DATGEM010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831963"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKQ27_16540"
     CDS             complement(<1..207)
                     /locus_tag="VKQ27_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY90592.1"
                     /translation="MNRFDAVIIGTGQAGPSLAARLAKAGMRVAIIERGKFGGTCVNN
                     GCTPTKALVASAYAARLAQRAAEYG"
     gene            complement(381..1586)
                     /locus_tag="VKQ27_16545"
     CDS             complement(381..1586)
                     /locus_tag="VKQ27_16545"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HLY90593.1"
                     /translation="MVEVKGHHDDGSDEDIADEVDFRPNVQGPVSAIDAASQSLIVLG
                     QTVTVSANTSFSDEISPASLLGVQVGDILEVSGMVAADGSIQATRVEREPAGSAFEVS
                     GVVSATDAIARTLKVNALVIDFAGATLNGFASGGPQDGQPVAATGAMLEQNGALQATQ
                     LQLLAGQDMREDQDDQAEVEGLITRFASASDFDVAGKPVSTSSSTEFDGGTAADLALN
                     LQVEVEGTVDSNGVMQAAKVRIGHPADVRLTAQVMAIDAGAGALTVLGTMVSVDASTR
                     FEDHSSADIQTFGLADIQVGDWLEIRGVAAGTNHTSIGAMRIDREQPQSAVHLMGPVV
                     SAAQPNLTILATAVTTNSQTQFSGLDPTSFFGTAVGKTVSVQGSWDGTTLTAATVQLG
                     DDHDDDGED"
     gene            1621..1764
                     /locus_tag="VKQ27_16550"
     CDS             1621..1764
                     /locus_tag="VKQ27_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90594.1"
                     /translation="MPLTVTIAALAWNCIPFRLNPALLKLLIVPTLTLALAAGIGFGD
                     MPP"
     gene            complement(2012..2860)
                     /locus_tag="VKQ27_16555"
     CDS             complement(2012..2860)
                     /locus_tag="VKQ27_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90595.1"
                     /translation="MTGASTGIGRRITERLAADGYFVFAGARKPEDLKSLGGPKNVQA
                     VRLDVTRQDEIDAVADLIGKSGRGLYGLVNNAGIGTVGTVADMKPEEFDLLMAVNLYG
                     PYRMTRAVEPLIVAAKGRVVNIGSISGIGFSSNLMAYSMSKHALEAFSESLFLQLKDS
                     GATASIVEPGNYNSDIGKNAIQRLGADAKALQDRGYRLAADRSRYKEPDEVADAVEKA
                     LFEPDPKRRYLVTPNQDEAEWAVSSLIGQLVQLNEGQRYTYSRDQLVKMLDEALAHAR
                     GRTSEP"
     gene            complement(2970..3043)
                     /locus_tag="VKQ27_16560"
     tRNA            complement(2970..3043)
                     /locus_tag="VKQ27_16560"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3009..3011),aa:Cys,seq:gca)
     gene            3248..>4792
                     /locus_tag="VKQ27_16565"
     CDS             3248..>4792
                     /locus_tag="VKQ27_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HLY90596.1"
                     /translation="MSTANAATAGGLFDKQRYIAGPGFSRWLVPPCALAIHLCIGMAY
                     GFSVFWLPLHKAIGITKSIDCPKDMSFLAEAFTTSCDWKVSSLGWMFTLFFVFLGTAS
                     ALWGGWLERVGPRKAGFAAAFLWCGGLLISAFGVTIHQLWLLWVGSGVIGGLGLGLGY
                     ISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGAPLADFLMKRFATTDSVGVWQTFVV
                     MAAIYFVYMIAGSLGYRLPAAGWKPAGWQPKAHTSLITARNVDLNTATVTPQFWLVWG
                     VLTLNVTAGIGIIGMASPLLQEVFGGRLIGVGKAFEQLSSEQHAAVATLAAGFTGLLS
                     LFNIAGRFCWASFSDYIGRKPTYMVFFLLGIALYASIPWTAAIGSMALFVGFFCIILS
                     MYGGGFATVPAYLADLFGTRMVGAIHGRLLTAWSAAGIFGPVIVNYIREYQLAHGVAH
                     AQVYTITMYILAAVLVLGLICNILVRPVADKYFMTPEQEAKLDAAVGAPAAPAATAPG
                     VGAVGSP"
BASE COUNT          773 a         1596 c         1517 g          906 t
ORIGIN      
        1 cccgtactcc gcggcccgtt gcgccagccg tgcggcatag gcgctcgcaa ccagcgcctt
       61 cgtcggggta cagccgttgt tgacgcaggt gccgccgaat ttccctcgct cgatgatcgc
      121 cacccgcatc ccggccttcg ccagacgcgc cgcaagggag gggcctgcct gtccggtgcc
      181 gatgatgacg gcgtcgaatc ggttcatgtg tgcacctcga gcgcgcagat gcgagcgcaa
      241 gcagcagccc ggatatcact tccgggtgcg gcacggagac agtacaccaa tggctgatcg
      301 gcgccatgcg ccctgtggag cctgtgggcc tcgaggccga gcaccggtcc cgggacatga
      361 ccccgggacc ggcagcaact tcagtcttcc ccgtcgtcgt cgtgatcatc ccccagctgg
      421 accgtggcgg ccgtcagcgt cgtgccgtcc cagctaccct gcaccgacac ggtcttgccc
      481 acggcggtgc cgaagaaact cgtcggatcc agtccggaga actgggtctg ggagttggtc
      541 gtcaccgcag tggcgaggat cgtaagattc ggctgcgctg cggatacgac cggccccatc
      601 aggtgtaccg cggactgcgg ctgctcgcgg tcgatgcgca tggcaccgat cgaggtgtgg
      661 ttcgtgccag cggcaacgcc ccggatctcg agccagtcac cgacctggat gtcggccagg
      721 ccgaaggtct gaatgtccgc tgagctgtgg tcctcgaagc gtgtgctggc atcgacgctt
      781 accatcgtac cgagcaccgt cagcgccccg gcgccggcgt cgatcgccat cacttgcgcg
      841 gtcagccgta cgtcggccgg atgcccgatg cgcaccttgg cagcctgcat gacgccgttc
      901 gaatcgaccg tgccttcgac ctcaacctgc aggttcagag cgaggtcggc ggcagtcccg
      961 ccgtcgaact cggtgctgga tgaggtgctc accggtttcc ccgcgacgtc gaagtcactc
     1021 gccgaagcga agcgcgtgat caggccctcg acctcggcct ggtcatcctg gtcctcgcgc
     1081 atgtcctgtc cggcgagcag ctgcagctgt gttgcctgca acgcgccatt ctgctcgagc
     1141 atcgcaccgg tggcggcaac cggctgacca tcctgggggc cgccgctggc gaagccgttg
     1201 agggtcgcac cggcgaagtc gatgaccaat gcgttgactt tgagcgtcct cgcaatggca
     1261 tcggtcgcgg agaccacacc gctgacctcg aatgcgctgc cggccggctc gcgctcgaca
     1321 cgcgtggcct ggatgctgcc atcggccgcc accatgccgc tcacctcgag gatgtcgccg
     1381 acctgcacgc ccaggagtga cgccgggctg atctcatcgc tgaaggaggt atttgccgag
     1441 accgtcacgg tctgccccag cacgatcagg gactgggatg cggcgtcgat cgcgctcacc
     1501 ggcccctgca cattggggcg aaagtcgact tcatcggcga tgtcctcgtc actgccgtcg
     1561 tcgtgatggc ccttcacctc gaccatgtca ccgacatgca gatcgccgag cgaagcgttc
     1621 ttgccgttga ccgtgacgat tgcagcgctt gcgtggaact gcattccatt caggctgaac
     1681 ccggcgctgc tgaaactgct gatcgtgccg acactgacgc tggctttggc ggcagggatc
     1741 ggcttcggtg acatgccgcc ataaccgccc ccaccgcaac ccgcgagcac catcgcgagc
     1801 atcacgagcg gtgccagcag cgctgaattg aaagctgcgc acgcaagagt gccgcggaac
     1861 tcacgccggt tcatggttgt tctccctgga gcgatcggag tgaccgcatc catgggtggt
     1921 gcatgcgtga gcagcggttc agcggaaaac tgtcgcaaaa ctgcggcagg aacgcccatt
     1981 tgagggtaaa ggcggaacgg ccaggcggtg ctcagggctc gctggttcgt ccccgcgcgt
     2041 gcgccagcgc ctcatcgagc atcttcacca gctggtcgcg gctgtaggtg taccgctgcc
     2101 cttcattcag ctgtacaagc tggccgatca gcgagctgac cgcccattcc gcttcgtcct
     2161 gattcggggt cacgaggtag cggcgcttcg ggtccggctc aaacaacgcc ttctcgaccg
     2221 cgtcggccac ctcgtccggt tccttgtacc gggaacgatc ggcggcgagg cggtagcccc
     2281 ggtcctgaag cgccttggca tccgctccaa gccgctggat cgcgttcttg ccgatatccg
     2341 aattgtagtt cccgggctcg acgatgctgg cggtcgcgcc cgagtccttc agctgaagga
     2401 agagagattc gctgaaggcc tcgagagcgt gcttgctcat ggaataggcc atgagattcg
     2461 atgaaaagcc aatcccggag atcgagccga tgtttacgac acgccccttc gcggcaacga
     2521 tcagcggctc gacggcccgg gtcatccgat acggtccata gagattgacc gccatcagca
     2581 gatcgaactc ctccggcttc atgtccgcga cggttcccac cgttccgata ccggcgttgt
     2641 tgaccaggcc gtagaggccg cggccgctct tgccgatgag gtcggccacc gcgtctatct
     2701 cgtcctgccg ggtaacgtcg aggcgcacag cctggacgtt cttcggccct ccgagggatt
     2761 tgagatcctc gggcttgcgc gccccggcaa acacgaaata accatccgcc gccagccgct
     2821 ctgtgatcct gcggccgatg ccggtgctcg cacccgtcac gagtaccgcc cgctgcgccg
     2881 ttggtacggc tggctctgcg actgcgagca gcggccatgc cagcagcaga aggaacgcca
     2941 atcgagtgcg tcggttcatg gggaatcctt ggaggctagg gtcggaatcg aaccggcgta
     3001 gacggctttg caggccgctg catgaccact ctgccaccta gcctcacatt gtgatgtttc
     3061 gtacctatgc gggggccgat acgaaaacgc cccgcacggc ctgtcgccgg cgaggcttgc
     3121 gccacatggg gcgctggctc tagcctaaca aaaagtgaac aatgggtcga ttgaccctcg
     3181 gggcggcgac tgcaccgctg cacgagggag gttcccgcca gctacgaaca agattaagga
     3241 gcgaggaatg tcaacagcga acgccgcaac cgccgggggc ctcttcgaca agcaacgcta
     3301 catcgccggc cccggcttca gccgctggct ggtgcctcca tgcgcactcg cgatccacct
     3361 gtgcatcggc atggcctacg gcttctcggt gttctggctg cctctgcaca aggccatcgg
     3421 catcacaaag tcgatcgact gcccgaagga catgagtttc ctcgccgagg cattcaccac
     3481 cagctgcgac tggaaggtgt cctcgctcgg ctggatgttc acgctgtttt tcgtgttcct
     3541 cggcaccgcc tcggcgctct ggggcggctg gctcgagcgc gtcgggccgc gcaaggcggg
     3601 tttcgccgcc gcctttctct ggtgcggcgg actgctgatc tcggcgtttg gcgtgaccat
     3661 tcaccagctg tggctgttgt gggtgggctc gggcgtcatc ggaggactgg ggctcggtct
     3721 gggttacatc tccccggtct ccacgctcat caagtggttc cccgatcgcc gcggcatggc
     3781 cactggaatg gccatcatgg gtttcggcgg aggggccatg atcggcgcac cgctggcaga
     3841 ctttctgatg aagcggttcg ccaccacgga ctcggtcggc gtatggcaga cgttcgtggt
     3901 gatggcggcg atctatttcg tgtacatgat cgccggctcg ctcggctacc gcctgcctgc
     3961 tgcgggctgg aagcccgccg gctggcagcc aaaggcgcac acctccctga tcacggcgcg
     4021 caatgtcgat ctcaacaccg ccaccgtcac gccgcagttc tggctggtct ggggtgtcct
     4081 gaccctcaac gtgaccgccg gcatcggcat catcggcatg gcatcgccgc tgctgcagga
     4141 ggtgttcggc ggacgactga tcggcgttgg caaggctttc gagcagctga gttccgagca
     4201 gcacgcagcc gtcgccacgc tcgcggccgg cttcaccggg ctcctgtcgc tattcaacat
     4261 cgcagggcgc ttctgctggg cctcgttctc ggactatatc ggccgcaagc ccacctacat
     4321 ggtgttcttc ctgctcggga tcgcgctcta cgcctcgatc ccctggaccg ccgcgatcgg
     4381 cagcatggcg ctgttcgtcg gcttcttctg catcatcctt tcgatgtacg gcggcggctt
     4441 tgcgaccgtg ccggcgtatc ttgccgacct gttcggtacg cgcatggtag gggccatcca
     4501 cggccgtctg ctcacggcct ggtcggcggc cggcatcttt ggtcccgtga tagtcaacta
     4561 catccgcgag taccagctgg cacacggtgt cgcacacgcg caggtctaca ccatcaccat
     4621 gtacatcctg gcggccgttc tggtgctcgg cctgatatgc aacattctcg tgcgaccggt
     4681 ggcggacaaa tatttcatga cgccggaaca ggaggcgaag ctcgacgccg cagtgggcgc
     4741 gcctgccgct cccgcggcaa ccgcgccggg cgttggggcc gtcggctcgc cc
//
LOCUS       DATGEM010000541         2547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_832007, whole genome shotgun sequence.
ACCESSION   DATGEM010000541 DATGEM010000000
VERSION     DATGEM010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832007"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VKQ27_16570"
     CDS             complement(<1..368)
                     /locus_tag="VKQ27_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90597.1"
                     /translation="MVSTYTTYRSQIHFALALIAYLMVLVGSVSIVKANPAASWGYFI
                     AVLPVVPAALVVWLFVRAIARLDEVQKRKHMQVFGFSLGGTALVTFGYGFLEGAGMPH
                     LNWTFVTPLMAMLWGLGLGIL"
     gene            836..1684
                     /locus_tag="VKQ27_16575"
     CDS             836..1684
                     /locus_tag="VKQ27_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90598.1"
                     /translation="MKVKGSHGAKPPKGGSALLFSHGGNVEVTPKVFIVYWGPQWANG
                     FSTGGYTSAQAQSYVEGFFGGVGGSSWSNSDTQYCQGVPSGTTTCPAGATFGTNPAGQ
                     LGGTWNDTGAVPATPTQSQIAAEAVAAAAHFGYNVNANYMVFTPTGMSSSGFGTRWCA
                     YHSSTSSSSGTVAYSYIPYMPDAGASCGMNFVNGTDDTFGHGYFDGYSIVAGHEYAEA
                     ATDPFPNSGWLDGSGQENADKCAWISSGQGASQNITLGANYYAVQSLWSNAFNGGAGG
                     CVISYP"
     gene            complement(1728..>2547)
                     /locus_tag="VKQ27_16580"
     CDS             complement(1728..>2547)
                     /locus_tag="VKQ27_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90599.1"
                     /translation="RQRLEFEDRLGLYERLDAHSMVVWMSFLMGDLAGADRESADMVS
                     RLLPGQAPYPALHLYAWRTITLHFLGRWDEAASMFWRDFEVWNEAGSHAAIYALRGFI
                     CGIDLGRARGDGRIEMAAQQAITSVLSRVAPTSGLQRLHDYMKGESAFTTGEWLGAYP
                     AEMVERRLNLANDCRQAVPDNVIEETMNRAVRYKIPLLEAQTRRTIALGRKDAQEMSN
                     AIAIWERIGAVPLIGRAKAERGLITGEEAETESGLQILSKLGDAFYLDRFSHQI"
BASE COUNT          486 a          865 c          760 g          436 t
ORIGIN      
        1 agaattccga gccccaaacc ccacagcatc gccatgagcg gcgtcacgaa ggtccagttc
       61 aggtgcggca tccccgcccc ctccaggaac ccatacccaa acgtcaccag cgcggtgccg
      121 cccagcgaga acccgaacac ctgcatatgc ttgcgcttct ggacttcgtc cagcctcgcg
      181 atcgccctga cgaacaacca caccaccagc gcggcaggca cgactggcag cacggcgatg
      241 aagtaccccc agctcgcggc agggttggct ttcacgatcg acaccgagcc caccagcacc
      301 atcagatacg cgatcagcgc cagcgcaaag tgaatctggg agcggtatgt cgtgtatgtc
      361 gagaccaaga tcgggggcgc cgatggtggc gattcaaacg ccacgggagg cggcgcttcg
      421 gtccgcacaa acggcgaggc cgcgtgggag aaatgggggt cgacgggcgg ctccgcctgg
      481 ggcggtggcg aggggttctg ctcagacact agcccgcgat actaacccgg cgggcgtccc
      541 atgggcttct tgacaggccg gctgagcaaa ccatctacca cgtcacgccc taccaacgca
      601 gtctgcccgc cggctggtcg attgttggca aatctaaaca attgatattt gactggttaa
      661 ggccatgcgg ctataaattt gcgcggctcc aggggagcgc tgcactcgga atccaaccgc
      721 aggagggctc catatcggcc gcatcgcccg acttggttta tctctactcg ccgcctcggt
      781 ccttctagct gcaaccgcag gcatatccag cgcgaaggac gccggcacca cctatatgaa
      841 ggtgaaaggc tcccatggcg ccaaaccccc caagggtggg tcggcgctcc tcttctcgca
      901 tggcggcaat gtcgaggtga ccccgaaggt cttcatcgtc tactggggcc cgcagtgggc
      961 aaacggcttc tcgaccggcg gctataccag cgcgcaggcg cagtcatatg tagaaggctt
     1021 ctttggcgga gtcggcggca gcagctggtc gaattcagac acccagtact gccagggcgt
     1081 gccatcgggt acgacaacct gtccggcggg cgccacgttt ggaaccaacc cggccggtca
     1141 gctcggcgga acctggaacg acaccggcgc ggtgcctgcc accccgactc agagccagat
     1201 cgccgccgag gcggtcgccg cggcggctca cttcggctac aacgtcaacg cgaactacat
     1261 ggtgttcacg ccgacaggta tgtcctcgag cggcttcgga accaggtggt gcgcctacca
     1321 cagctcgacc agctcgtcga gcgggaccgt cgcctactcc tatatcccct acatgccaga
     1381 cgccggtgcg tcatgtggca tgaacttcgt caacggcacc gacgacacct tcggccacgg
     1441 ttacttcgac ggatattcca tcgtcgccgg ccatgagtac gccgaggcgg ccaccgaccc
     1501 gttccccaac agcggctggc tggacggcag cggccaggag aacgcggaca agtgcgcctg
     1561 gatctcgtcg ggccagggcg cctcgcagaa catcaccctc ggcgcgaact actacgcggt
     1621 gcagtcgttg tggagcaacg ctttcaacgg cggcgcaggc ggctgcgtca tctcgtatcc
     1681 gtgatctagc gaaccgatcg agggccgccc gcaagggcgg cccttcttca tatctggtgg
     1741 gagaagcggt caaggtagaa ggcatcgccg agcttgctca ggatctgcag gcctgattcg
     1801 gtttcggcct cctccccggt gatcaatccg cgctccgcct tggccctgcc gataaggggc
     1861 acggccccga tgcgctccca gatcgcaatc gcgtttgaca tttcctgcgc atccttgcgc
     1921 ccgagcgcga tcgttcgtcg cgtctgggct tccagcagcg ggattttgta gcgcaccgct
     1981 cggttcatcg tttcctcgat gacgttgtcc ggaaccgcct gccggcagtc attcgccagg
     2041 ttgaggcgcc gctccaccat ctccgccgga taggctccca gccattcacc cgtggtgaag
     2101 gcggattcgc ccttcatgta gtcgtgcagg cgctgcaacc cgcttgtcgg agccacgcgg
     2161 ctgagcacag aggtgatcgc ctgctgcgcc gccatctcga tccggccatc tccacgcgcg
     2221 cggccgaggt cgatgccgca gatgaaaccg cgcaacgcgt aaatcgcggc atgcgacccg
     2281 gcctcgttcc atacctcgaa gtcacgccag aacatcgatg ccgcctcatc ccatcgcccg
     2341 agaaagtgca gcgtgatcgt tcgccacgca tatagatgga gggccggata cggtgcctgt
     2401 cccgggagca gcctggaaac catgtcggcc gactctctat ctgcacccgc caggtcaccc
     2461 atgaggaacg acatccacac caccatcgag tgcgcgtcga gccgctcgta caacccgagg
     2521 cgatcttcga actcgagacg ctgccgc
//
LOCUS       DATGEM010000542         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1461754, whole genome shotgun sequence.
ACCESSION   DATGEM010000542 DATGEM010000000
VERSION     DATGEM010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461754"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VKQ27_16585"
     CDS             complement(<1..444)
                     /locus_tag="VKQ27_16585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY90600.1"
                     /translation="MMIVDAQIHLWAKGTPSAHHRQTPFLKEEALALMDEAGVDRAVV
                     HPVLWDPDSNELAVEAAREHPDRFAIMGWFYLDDPAQRATIETWKSRPGMKGLRFYFS
                     DPRSLTWADDGNLDWLLPVAERLDIPVSLQASAFLPRVGELAERYP"
     gene            complement(499..1515)
                     /locus_tag="VKQ27_16590"
     CDS             complement(499..1515)
                     /locus_tag="VKQ27_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HLY90601.1"
                     /translation="MSDRIHLSVVEARALGEAAMRGAGYDQEDARILTDHVLDAALCG
                     YEYSGLPKLLNVVDYADFKRARHPITVIRETGATALLDGGNNAGMVAAYRASEAAIAR
                     GETHGLAIVCMGNTWMTGRSAYYCEMIARAGLVAIHTVAAPPAVAPFGGARAALGTNP
                     IAFGFPTEGDPLVIDMGTSAFMGTDLQFRARMGIPIPEGVALGPNGGPTTNAALARQG
                     TLLPFGGPEGGYKGFGLALAMDALGVLAAGAQSEGSISGYLFIALKPDLFVSADEYRH
                     DVSQRIATIKATPRQAGVTEIRIPGERSYATRARLMHEGIEIDKKIYNALGRLAKGLL
                     DHGG"
     gene            1596..2591
                     /locus_tag="VKQ27_16595"
     CDS             1596..2591
                     /locus_tag="VKQ27_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HLY90602.1"
                     /translation="MRILWPNLPAEYRPVALEAIGSGFETDFCATPAEVTDAQWASAE
                     AIVGNFPSQYIDKLRNCRIFVKYGVGYDDVDIERFGKLGIPVCNTPDYGTREVADHAI
                     ALMLTLSKSIAYHNAELHADLRGNWRPAHNPFGHRLSVCTYGVVGMGRIGTAAARRAK
                     AFDMDVVFYDPYQPNGYQFAIGVRRADSLAELMGQCDFVSIHTPLTSETRNMMGAEAF
                     AAAKRGMILINTARGPIVDIDALYDAMKDGTILAAGLDVLPQEPANPQSRLIAAWQAN
                     EDWIRHRLILTPHSAFFTPESMRDNRGFASRTAARYLRDGRLENCVNKQFLVSRT"
     gene            2723..>4255
                     /locus_tag="VKQ27_16600"
     CDS             2723..>4255
                     /locus_tag="VKQ27_16600"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLY90603.1"
                     /translation="MNEKVDVLIIGSGASGAAVAWSLAETKMRILCLEQGEWMKQSDY
                     PSNGRDYEARVFSDYATSPNIRKRPSDYPINDDNSPIKVVNFNAVGGSTVMYTAHYPR
                     LHPSDFRVRTLDGVADDWPIDYATLEPFFAENDRMTGVSGLAGDPAYPSHEPPMPPLP
                     LGKTGAKIGKTMNQLGWHWWPSDSAIATTEYDGRAPCINLGHCTPACAQGAKASTDIT
                     YWPHAIRAGVELRTNCRVRQILTGPDGMATGVVYYDVNGTEQFQPAEVVIMACNGVGT
                     PRLLLNSTSARFPNGLANSSGLLGKNLMFHPWAHVSGYVDEPMDGHRGPPLCLWSQEF
                     YETDPNRGFVRGFNFQFNRTMGIGTEAMNAEANGLLPWGEAHHDTFRAMVGHRMSVGI
                     CTEDLPEEHNRVTLDPVLKDRHGIPAPRIDYTISENTNRMLDFAIERGREILAAANAR
                     QVHSVRPVVYGGWHLLGTARMGNDPERSVVNPWGRSHDVKNLFIVDGSIFVTSGGVNP
                     TCT"
BASE COUNT          812 a         1552 c         1163 g          728 t
ORIGIN      
        1 cggataccgc tccgcaagct cgcccacacg cggcagaaac gcggaagcct gcagcgaaac
       61 cggaatgtcc agccgttccg ccactggcaa cagccagtcc aggttgccgt cgtccgccca
      121 tgtcaacgat cgagggtcag agaagtagaa ccgcagcccc ttcatccccg gccggctctt
      181 ccaggtctcg atcgtggcgc gctgtgccgg atcgtccagg tagaaccagc ccatgatcgc
      241 gaagcggtcg ggatgttcgc gtgccgcttc aaccgccagt tcattcgagt ccggatccca
      301 aagcaccggg tggacgacag cacgatcgac ccccgcctca tccatcagag ctaatgcctc
      361 ctccttcagg aagggcgtct ggcgatgatg ggcagagggc gtacccttcg cccacaggtg
      421 aatttgcgca tcgacgatca tcatggcagt tggccttctt gtgacgcggt gacaggctat
      481 tcggacggag cgcgcttctc aaccaccatg gtcgagaagc cccttggcga gccgaccgag
      541 cgcattgtat atcttcttgt ctatctcaat accttcgtgc atcagacgtg cacgcgttgc
      601 atagctgcgc tctccgggga tgcggatttc cgtgacaccc gcctgccgtg gcgtcgcctt
      661 gatcgtcgcg atgcgctggc tgacatcgtg acgatactca tctgccgata cgaacaggtc
      721 cggcttcagc gcgatgaaca gatatccgct gatcgagcct tctgactgcg ctcctgccgc
      781 cagcaccccc aacgcatcca tcgccagagc cagcccgaaa cccttatagc cgccctccgg
      841 cccgccaaac ggcaacagcg tgccctgccg tgccagtgct gcattcgttg tcggcccgcc
      901 attcggcccg agcgcaacac cttccgggat cggtattccc atgcgcgccc ggaactgcag
      961 atctgtaccc atgaaggccg aggtgcccat atcgatcacc agcggatcgc cctcggtcgg
     1021 gaacccaaat gcgatcggat tcgtccccag tgccgcgcgt gccccaccga acggagccac
     1081 cgctggcggc gcggccaccg tatggatcgc caccaacccg gcacgcgcga tcatctcgca
     1141 gtaatacgcg ctacgccccg tcatccacgt attgcccatg cagacgatcg ccaatccatg
     1201 cgtctcgcca cgcgcgatgg ctgcctcgga agcgcgatac gccgccacca tccccgcgtt
     1261 gttgccgcca tccagcagcg ccgttgcgcc tgtttctcgt atcaccgtga tggggtgccg
     1321 ggcccgcttg aaatcggcat aatcaaccac gttcagcagc ttaggcagcc cggaatactc
     1381 gtacccgcac agcgccgcat ccagaacgtg atcggtcagg atacgcgcat cctcctggtc
     1441 gtatccggct ccacgcatcg ccgcctcgcc gagcgcacgc gcctcgacca cgctcaaatg
     1501 gatgcgatcg ctcatcggct tcccctcgat tggtctcggg cgcgcatggt ggcactatgc
     1561 ggcgcaacat caagcgatgg ggaaacgaaa tggcgatgcg cattctttgg ccgaaccttc
     1621 ccgccgaata tcggccggtt gcccttgaag cgatcggctc cggtttcgag accgatttct
     1681 gcgccactcc cgccgaggtg accgacgcgc aatgggccag tgccgaagcc atcgttggca
     1741 acttcccgtc gcaatacatc gacaagctgc gcaactgccg catcttcgtg aaatacggcg
     1801 tcggctacga cgacgtggac atcgagcgct tcggcaagct tggcatcccc gtctgcaaca
     1861 cgcccgacta cggcacccgc gaggtcgccg accacgccat agccctgatg ctgacgctgt
     1921 ccaaaagcat cgcctatcac aatgccgaac tgcacgccga cctccgcggt aactggcgcc
     1981 ccgcgcacaa tccgttcggc catcgcctgt cagtctgcac ctacggcgtc gttggcatgg
     2041 gccgtatcgg caccgccgcc gcccgccgcg ccaaggcgtt cgacatggac gtggtcttct
     2101 acgacccata ccagccgaac ggctaccagt tcgccatcgg cgtccgccgc gctgattcgc
     2161 ttgccgaact gatgggccag tgtgatttcg tcagcatcca cacgccgctg acatcggaaa
     2221 cccgcaacat gatgggcgcg gaagctttcg ccgccgccaa gcgcgggatg atcctgatca
     2281 acaccgcccg cggtcccatt gtcgacatcg acgccctgta tgatgcgatg aaggacggca
     2341 cgatcctcgc cgccggcctc gacgtgctcc cgcaggaacc cgccaacccg caatcccgcc
     2401 tgatcgccgc ctggcaggcc aacgaagact ggatccgcca tcgccttatc ctgacgccgc
     2461 actccgcctt cttcaccccc gagagcatgc gcgacaaccg cggctttgcc tcccggacag
     2521 cggcgcgcta tctccgcgac ggtcgcctgg agaactgcgt caacaagcaa ttcctggtgt
     2581 cgcgcaccta acccctgtca ttgcgaggag acgcagccga cgaagcagtc tcgaacccgc
     2641 actcgcgcac cgatcggtcg ggattgcttt gtcgcaatgc tcgcattaac tggatgatac
     2701 gcgtatatcc aaagaggcat acatgaacga aaaggtcgac gtccttatca tcggctctgg
     2761 cgcctcaggc gccgccgtcg cctggagcct cgccgaaacc aagatgcgta tcctctgcct
     2821 ggaacagggc gagtggatga agcagtccga ctacccctcc aatggccgcg actacgaagc
     2881 ccgcgtgttc agcgactacg ccacgagccc gaacattcgc aagcgcccca gcgattaccc
     2941 gatcaacgac gacaactccc cgatcaaggt ggtgaacttc aacgccgtgg gcggctccac
     3001 cgtgatgtac accgcgcact acccacgcct gcacccctcc gacttccgtg tccgcacgct
     3061 cgacggcgtc gccgacgact ggcccatcga ctacgccacc ctcgagccat tctttgccga
     3121 gaacgatcgc atgaccggcg tgtccggcct tgccggcgat cccgcctatc ccagccacga
     3181 accgcctatg ccgccgctcc ctctcggcaa gaccggcgcc aagattggca agacgatgaa
     3241 ccagctcggc tggcattggt ggccatccga cagcgcgatc gccacgactg agtacgacgg
     3301 ccgcgccccc tgcatcaatc tcggccactg cacccccgct tgcgcacagg gcgccaaggc
     3361 cagcaccgac atcacctact ggccgcacgc catccgcgcg ggtgtcgaac tccgcaccaa
     3421 ctgccgcgtc cgccagatcc tcaccggccc agacggcatg gcgaccggcg ttgtctacta
     3481 cgacgtcaac ggcaccgagc aattccagcc cgccgaagtc gtcatcatgg cctgcaacgg
     3541 cgtcggtacg ccacggctgc tgctgaactc aacctccgcc cgcttcccca acgggctcgc
     3601 caattccagc ggccttctcg gcaagaacct gatgttccac ccctgggcgc acgtcagcgg
     3661 ctatgtggac gaaccgatgg acggccatcg cggcccgccg ctctgcctgt ggagtcagga
     3721 gttctacgaa accgacccga accgcggctt cgtccgcggc ttcaacttcc agttcaatcg
     3781 caccatgggc atcggcaccg aggcgatgaa cgccgaggcc aatggcctcc tcccctgggg
     3841 cgaagcccac cacgatacct tccgcgccat ggtcggccac cgtatgtctg ttggtatatg
     3901 caccgaagac ctgcccgagg aacacaaccg cgtcaccctg gatcccgtgc tgaaggatcg
     3961 ccacggcatc cccgcccccc gcatcgacta caccatcagc gaaaacacca atcgtatgct
     4021 ggatttcgcc atcgagcgcg gcagggaaat cctcgccgcc gccaacgcgc gtcaggtcca
     4081 ctccgtgcgt cctgtcgtct acggcggctg gcacctgctg ggtaccgccc gcatgggcaa
     4141 cgacccggaa cgctcggtgg tgaatccctg gggccgcagc cacgacgtga agaacctgtt
     4201 catcgtggat ggcagcatct tcgtcacctc aggcggcgtg aacccgacct gtacg
//
LOCUS       DATGEM010000543         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1222951, whole genome shotgun sequence.
ACCESSION   DATGEM010000543 DATGEM010000000
VERSION     DATGEM010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222951"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1459)
                     /locus_tag="VKQ27_16605"
     CDS             complement(185..1459)
                     /locus_tag="VKQ27_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY90604.1"
                     /translation="MNLHRLLGKRLEAGRPVRVGLIGAGKFGSMFLAQVPSSPGIEVA
                     AIADLAPDRAREACRRVGWTDERIAATGFTDDAAMMLKAPDLEVVVEATGHAPAGIRH
                     ARCAIAEGKHIVMVNVEADVLAGIALAREAARAGVVYSMAYGDQPALICELVDWARAC
                     GFRILAAGKGTKYLPEYHASTPATVWSYYGLTAEQARAGGMNPQMFNSFLDGTKSAIE
                     MAAVANATGLDVPADGLAFPALGSHQLADGLGDRAGQVEVVSSLHRDGSVVPNDLRWG
                     VYVVFEAPSDYTARCFAEYGVATDASGRISALYRPFHLIGLELNISILSAALRGEPTG
                     SPTAFRGDVVATAKRDLREGEVLDGEGGSCVWGRLMPAQASLAIGGLPIGLANHVTLR
                     RDVPLGQCVTWDDVRVDETDEAYRYRREMERL"
     gene            1527..2315
                     /locus_tag="VKQ27_16610"
     CDS             1527..2315
                     /locus_tag="VKQ27_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLY90605.1"
                     /translation="MAGLPEVEWRKLRADELRDLARADAIVILPVASLEQHGPHLPVE
                     VDSMLGETVATRAARKLAERGQRAVVLPVLWTGLSEHHMSFGGTVTLDFPAFAAVVEG
                     VCRSVLRHGFKRIVLLNAHGGNENALRTITDELTPKLGAPIVQFTYWYAAAAAIAKIL
                     DTQGALMHACEAETSMMMAVRPELVAMDRVGMAKANSTPDVSDVAGGGVYRWRTIAAR
                     SSSGVIGNPEAATAEKGERLFEAIATALADKLGNKELWELPWQA"
     gene            2436..>3583
                     /locus_tag="VKQ27_16615"
     CDS             2436..>3583
                     /locus_tag="VKQ27_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY90606.1"
                     /translation="MPTGKWQFWIDRGGTFTDIVARHPDGELSTHKLLSENPGRYRDA
                     AIAGIKTVLGLPLDAPIPSGLVEAVKMGTTVATNALLERKGERTLLVVNRGFADALRI
                     GNQARPRLFDLAITLPSMLYEEVVEIGGRVGIDGVEVEPLDEAGARQAFAEVRAKGIA
                     ACAIVLMHAWKYPEHERRLAELAREAGFTQVSASHVVSPLLRLVPRGDTTVVDAYLSP
                     ILRRYVDQVASELGDVRLYFMQSNGGLADAAGFQGKDAILSGPAGGIVGAARTAAMAG
                     LDHIIGFDMGGTSTDVALYAGGFERAFETAVAGVRMRAPMMAINTVAAGGGSILHFDG
                     ARFRVGPDSAGAEPGPACYRNGGPLTVTDANVCVGKIQPRHFPAIFGQ"
BASE COUNT          657 a         1152 c         1167 g          607 t
ORIGIN      
        1 ctcagctatg tcgatacaac catggctacg ccggaactga atgccttcat gggctgaccg
       61 ctgtaatgaa ctccctccaa tgtcattgcg acgagcgaag cgacgaagca agctcggtca
      121 agacacgacc agcccctcac atgatcgagc tggcttcgtc gctgcctcct cgcaatgaca
      181 gctgctacaa tcgctccatc tcccgtcgat accggtacgc ctcatccgtt tcgtccacgc
      241 gcacatcatc ccacgtgacg cactgaccaa gcggcacgtc acggcgcagg gtcacatggt
      301 tcgccagccc gatcggcaag ccacctatag ccagactagc ctgggccggc atcagtctcc
      361 cccagacgca actgccaccc tccccgtcca gcacctcgcc ttcgcgcagg tcccgcttcg
      421 cggtcgcgac cacgtcgccg cggaacgcag tgggcgagcc tgttggctcg ccacgcaaag
      481 cggcagacag gatcgagatg ttgagctcca ggccgataag gtggaacggg cgatacagcg
      541 ccgatatgcg gccggacgcg tcagtcgcga cgccgtattc cgcgaagcag cgcgcggtgt
      601 aatcgctggg cgcctcgaac acgacataga cgccccagcg cagatcgttc ggcacgacgc
      661 tgccgtcacg atgcaggctg gatacgactt cgacctgccc ggcccgatca cccagaccgt
      721 ccgcaagctg gtgcgaacca agcgcgggaa acgccaggcc atcggccggc acatccagcc
      781 cggtggcatt ggcgaccgcc gccatctcaa ttgctgattt ggtgccatcc aggaacgagt
      841 tgaacatctg cgggttcatg ccaccggcgc gcgcttgttc cgcagtcagg ccgtagtagc
      901 tccagacggt ggcgggagtg gaggcatgat actccggcag atatttcgtc cccttccccg
      961 ccgccaggat gcggaagccg caggcacgtg cccaatcgac cagttcgcag atcagtgccg
     1021 gctggtcgcc ataggccatc gaatacacca cgccagcccg tgcggcttcg cgggccaaag
     1081 cgatgccggc gagcacgtcg gcttcgacat tgaccatgac gatatgcttg ccctcggcga
     1141 tcgcgcatcg cgcgtgcctg atgcccgccg gggcatggcc ggtggcttcc accacgacct
     1201 cgaggtcggg cgccttcagc atcatggccg catcgtcggt gaacccggtg gccgcgatgc
     1261 gctcgtcggt ccagccaacg cgacgacagg cctcgcgagc acggtccggg gcaagatcgg
     1321 cgatcgccgc gacctcaatc cctggcgagg acggcacctg ggcaaggaac atcgagccga
     1381 acttgccggc cccgatcagg ccgacccgga caggacggcc tgcctccagg cgcttgccga
     1441 gcagccgatg caaattcatt catgcctccc tttcctcgtc cggcgtttcg tggcccattg
     1501 ctacgccagg accgggagat tggcgaatgg caggattgcc tgaagtggaa tggcgcaagc
     1561 tgcgcgctga cgaattgcgc gacctggcgc gcgccgacgc gatcgtgatc ctgccggtgg
     1621 cgtcgctcga gcagcacggg ccacacctgc cggttgaagt cgactccatg ctgggcgaga
     1681 cggtcgccac gcgcgcggca cgcaagctgg cggaacgcgg acagcgtgcg gtggtgttgc
     1741 cggtgctctg gactgggctc tccgagcatc acatgagttt cggcggcacc gtcacgctcg
     1801 atttcccggc atttgcagcc gtggtggaag gtgtctgccg gtccgtgctg cggcacgggt
     1861 tcaagcggat cgttttgctg aatgcgcatg gcggtaacga gaacgcgttg cgcaccatca
     1921 ccgacgagct gacaccgaag ctcggtgcgc cgatcgtcca gttcacctat tggtacgctg
     1981 ctgccgccgc gattgcgaag atactggata cgcagggtgc gttgatgcat gcgtgcgagg
     2041 ccgaaacgtc catgatgatg gcggttcgac cggagctggt agcgatggac cgcgtcggta
     2101 tggccaaggc gaacagcacg ccggatgtca gcgatgtggc gggcgggggc gtgtatcgct
     2161 ggcgaacgat cgccgcgcgg tcgtcatcag gcgtgatcgg aaatcccgag gcggcaacag
     2221 cggaaaaggg cgaacgattg ttcgaggcaa ttgccacggc gctggctgac aagctgggca
     2281 acaaggaact gtgggaacta ccgtggcaag catgactgtc attgcgagga gcaaagcgac
     2341 gaagcaagct cgggcaagct tcacgcgcac cgacagtcca agattgcttg gccggccgca
     2401 cctccgcgca atgacagatc agcgtggggg caatgatgcc gacaggtaag tggcagttct
     2461 ggattgatcg cggcggcacc tttaccgaca tcgtcgcacg ccatccggat ggcgagctct
     2521 caacccataa gctgctgagc gaaaaccccg ggcggtatcg cgacgccgcc atcgccggca
     2581 tcaagacggt gctgggccta ccgctcgatg cgccgatccc ctcaggcctg gtggaagcgg
     2641 tgaagatggg caccaccgtt gccaccaacg cgctgctgga acgcaagggc gaacgcacgc
     2701 tgctggtggt gaaccgtggc ttcgccgacg cgctgcgtat cggtaaccag gcgcggcccc
     2761 ggctgttcga tctggcgatc actctgccct cgatgctcta cgaagaagtc gtggagatcg
     2821 gcgggcgtgt cggcatcgat ggcgtcgagg tggaacccct ggacgaagca ggtgcgcggc
     2881 aagcttttgc cgaggtacgt gcgaagggca tcgcggcctg cgccatcgtg ctgatgcatg
     2941 catggaagta tcccgaacac gagcgccgtc tcgccgaact ggcgcgcgag gctggcttca
     3001 cccaggtgtc cgcaagccac gtggtgagcc cgctgctgcg actggtgccg cgcggcgaca
     3061 ccactgtcgt cgatgcgtat ctgtcgccga tcctgcgacg ttacgtggat caggttgcat
     3121 cggaactcgg cgatgtccgg ctgtatttca tgcagtccaa cggtgggctg gccgatgcgg
     3181 cgggcttcca gggaaaagac gcaatccttt ccggccctgc cggcggcatc gtcggtgcgg
     3241 cacgcaccgc cgcgatggcg gggctcgatc acatcatcgg cttcgatatg ggcggcacct
     3301 cgactgatgt ggcgctctac gccggcggct ttgagcgtgc attcgagacg gcagtggccg
     3361 gcgtgcgcat gcgcgcgccc atgatggcaa tcaacacggt cgcagcaggt ggcggatcca
     3421 tcctgcactt cgatggtgcg cggttccggg tgggccccga cagtgcgggt gccgaaccgg
     3481 gaccggcctg ctaccgcaac ggtggtccgc tgacggtcac cgatgcgaat gtctgtgtcg
     3541 gcaagatcca gccccggcat ttcccggcga tcttcgggca gaa
//
LOCUS       DATGEM010000544         6563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_570130, whole genome shotgun sequence.
ACCESSION   DATGEM010000544 DATGEM010000000
VERSION     DATGEM010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570130"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1772
                     /locus_tag="VKQ27_16620"
     CDS             <1..1772
                     /locus_tag="VKQ27_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY90607.1"
                     /translation="SFARLLLASQAEHHGPPRGFFGAFDRAFNRFRDGYGRMLEGALH
                     RRGLVLLCAAGLLIISGGLTAVIGLDFFPSADVGLIKLHFRAPPGTRIERTEQLVLQV
                     EDRLRQIIPPSEIDTINDTVGALSSFNMAFVPTDNVGDMDAEILISLKPGHHPSAGYI
                     HAIRTKLPDEFPGSLFYFQTADIVSQVLNFGLSAPIDVQVQDVNFDRAAELARKLLAR
                     MQRIPGVADAHMVQVLNYPSLQVEVDRLRAAKLGVSQRDVASNMLTTLSSSSLVAPNF
                     FLNPQNNVNYFVAVQTPIERITSVNDLLDTVVNRPPADGAGPAAQPGAPVIRLGDVAS
                     VSPRSSLESVSHYTVQRELDIAANVDGRDLGSTAADIQQAINDISKGLPITTKILILG
                     QNDVMQSSFRSLGLGMILAVLLVYALLVILFQSWVDPLIIMMAVPGALMGILWMLALT
                     HTTINVESLMGTIMSIGISVSNSILVVSFANDLRAREEVGPLRAVIEAGRIRLRPVLM
                     TALAMIIGMVPMALGLGEAGEQNAPLGRAVIGGLIVATLSTLFIVPIFYTLLRRKPPA
                     LHTLDTRFAAEAAGAQTTGGSHG"
     gene            1765..2883
                     /locus_tag="VKQ27_16625"
     CDS             1765..2883
                     /locus_tag="VKQ27_16625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY90608.1"
                     /translation="MARRGRSGSFLLYLIGLLVVAGAAFGGLHLWWQKDAQLIASREA
                     LADSIAQGPAVQVATITQGPKERTITLLGDARPYQVATLYGKVSGYVKSIQVDRGDHV
                     KAGDILAVVTSAETDRQYDAGVSDMENKRKNAERERDLVARGWTSVQASDAAETAYRM
                     AKASVAQLEAMKDYEILRAPFDGTVTARYVDVGALVQSSVTNQTSNQPVVMVADMSKL
                     RVDVYVEQGDVPYVHVGDVADVADAADASRHVRARIARTSDQLDPRTRTLFVELDVDN
                     SENFLVPGSFAYVTLHAPVASYPEIPIAALITRGAKTFVADVSKDSLVHLQAVKIANT
                     DGIVASVADGAKVGDRVALNLPDEVTDGGRIRPVETSH"
     gene            2846..4870
                     /locus_tag="VKQ27_16630"
     CDS             2846..4870
                     /locus_tag="VKQ27_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLY90609.1"
                     /translation="MAAGSAPLKPATDTIYPVKVHTAIRVTMRDGVELNVRVTRPDAP
                     GRFPGVMEYHPYRRLGAALPDYRDEYPPVVPYLAERGYAVVQFDVRGTGSSSGYSTDI
                     YSPEERQDGYDMVAWIAAQPWCTGAVGMIGKSYGAVVQWQVAVQRPPALKAIVVRSAN
                     DDVYTGFTNPGGCIRPWMFEYYAPLMNALNFAPPDPALVGDRWAAIWAERLEHSAPWS
                     LGYIRNTLHGPYWRDQSLAPDYDRVECAVLLIEGWADWYATEELRAFQRLNTPKKVLI
                     GPWGHYYAEEQQAFPGPRIDARREYLHWFDHWLKDIDNGVMSEPPVTVFVRGWQAPSL
                     LCLEDTGAWRNESTWPPERLHPTTLYLSEGGMLTDTAGNSGSDSYQYRPSVGITTGRR
                     GLGSTTPWAMPIDQRLDEAHSLLFTTEPLRQDLELLGQPEALLHIASTAEVAYFHMKL
                     CDLAPDGASRLICDGGLLATHRNSHETPEKLAPGQVYALRIPLRHCAYAIQTGHRLRI
                     AIASAEFQNAWPTGQHAVNTIFRGGHHASHIVLPVAPRDAHPPAPPEFAASPHALPAA
                     DSLAPPGYTFELDLVNDTVTCALRAPEGGRTSHHSRYTVSNHDPAHTTIIGSAKHTAV
                     HPTLDIRIEATCQTTSDADTYSHLSQVRITVDGAPHFSKSWSESVPRKLS"
     gene            complement(4877..5812)
                     /gene="thyX"
                     /locus_tag="VKQ27_16635"
     CDS             complement(4877..5812)
                     /gene="thyX"
                     /locus_tag="VKQ27_16635"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HLY90610.1"
                     /translation="MRLTEQQAAEIATARAQRAETARPTVAALEAMLFEAIPVLDHGF
                     VRVIDYMGDDSAIVQAARVSYGRGTRRVSEDAGLIRYLMRHRHSTPFEMCEIKYHVKL
                     PIFVARQWIRHRTANVNEYSARYSILDREFYLPAPEHLAAQSVANRQGRGDVLAGEEA
                     ARVLDMLREDAIRCYDHYADMLNEDEAGVPRDPSRQGLARELARMNLTLNTYTQWYWK
                     IDLYNLLHFLSLRADAHAQYEIRVYADAMLRSVAAWVPVAHQAFLDYRLGAVTLSAQM
                     LTAVRRMLADEAVDQANSGLNRREWAELMAALGRS"
     gene            5821..6162
                     /gene="ssrA"
                     /locus_tag="VKQ27_16640"
     tmRNA           5821..6162
                     /gene="ssrA"
                     /locus_tag="VKQ27_16640"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT         1159 a         2307 c         1942 g         1155 t
ORIGIN      
        1 cgagcttcgc gcgcctgctg ctggcatcgc aggccgagca tcatggtccg ccacgcggtt
       61 tcttcggcgc cttcgaccgc gccttcaacc gcttccgcga cggctatggc cggatgctgg
      121 aaggcgcact gcaccgccgc ggcctcgtgc tgctctgcgc cgccggcctg ctgatcatct
      181 cgggtggcct gactgccgtc atcggcctcg acttcttccc ctccgccgat gtcggcctga
      241 tcaagctgca tttccgcgcg ccgcctggca cacgcatcga gcgcaccgag caactggtgc
      301 tccaggtcga agaccgtctg cgccagatca ttccgccgag cgaaatcgac accatcaacg
      361 acaccgtggg cgcgctctct tcgttcaaca tggccttcgt gccgaccgac aatgtcggcg
      421 acatggatgc cgagatcctg atctcgctga aaccgggaca tcatccctcg gccggataca
      481 tccacgccat ccggaccaag ctgcccgacg aattccccgg cagcctgttc tatttccaga
      541 ccgccgacat cgtcagccag gtactgaact tcggcctgtc cgcaccgatc gatgtgcagg
      601 tgcaggatgt caacttcgat cgtgcggcgg aactggcgcg caaactcctg gcgcgcatgc
      661 agcgcattcc gggcgtcgcc gacgcgcaca tggtccaggt gttgaactat ccctcactgc
      721 aggtggaggt cgatcgcctg cgcgcggcaa agctcggtgt ctcgcagcgc gacgtggcca
      781 gcaacatgct gacgacgctg tcctccagtt ccctcgtggc acccaacttc ttcctcaatc
      841 cccagaacaa tgtgaactac ttcgtggcgg tgcagactcc gatcgagcgg atcacgtcgg
      901 tgaatgacct gctggacacc gtcgtcaacc gaccgcccgc ggatggcgcc ggtcccgctg
      961 cccagcctgg tgcgccggtc atccgcctcg gcgacgtcgc atccgtgtcg ccgcgatcca
     1021 gcctcgagtc ggtcagccac tacacagtgc agcgcgaact cgacatcgca gcgaacgtcg
     1081 atggccgcga tctcggctcc accgccgccg acatccagca ggcgatcaac gacatcagca
     1141 aaggccttcc aatcaccacc aagatcctga tacttggcca gaacgacgtg atgcagagct
     1201 cattccgcag ccttggcctg ggcatgatcc tggcggtgct gctggtctat gcgctgctcg
     1261 tcatcctgtt ccagtcctgg gtcgatccgc tgatcatcat gatggcggtg cctggcgcgc
     1321 tgatgggcat cctgtggatg ctggccttga cccacacgac gatcaacgtt gagtcgctga
     1381 tgggcaccat catgtccatc ggcatctccg tctccaactc gatcctggtt gtaagcttcg
     1441 ccaacgatct gcgcgcgcgt gaggaagtcg gcccgctccg cgcggtgatc gaggccgggc
     1501 gtatccgcct ccgccccgtg ctgatgaccg cgctcgccat gatcatcggc atggtgccca
     1561 tggcgctggg cctcggcgag gcgggcgagc agaacgcgcc gctcggccgc gcggtaatcg
     1621 gcggccttat cgtggccacg ctctccacac tcttcatcgt gccgatcttc tacacgctgc
     1681 tgcggcgcaa gccgcccgcc ttgcacaccc tcgatacgcg gttcgccgcc gaggccgcgg
     1741 gcgcacaaac aacagggggc tcgcatggct agacgcggcc gatccggctc gtttctgctg
     1801 tatctcatcg gcttgctggt cgtggccggc gcagccttcg gcgggctgca cctttggtgg
     1861 cagaaggatg cacagctcat cgcgtcacgc gaggcgctcg cggacagcat cgcacagggt
     1921 cccgcggtgc aggttgccac gatcacgcag ggccccaagg agcgcacgat cacgctgctc
     1981 ggcgatgcgc ggccgtacca ggtcgccacg ctgtatggca aggtgagcgg ttacgtgaag
     2041 tcgatccagg tcgatcgcgg cgatcacgtg aaagcagggg atatcctggc cgtggtcacc
     2101 tcggcggaga ccgatcgcca gtacgacgcc ggcgtcagcg atatggagaa caagcgcaag
     2161 aatgccgagc gcgagcgcga tctggtcgcg cgcggctgga cgtctgtgca ggcgtccgac
     2221 gccgcggaaa ccgcgtatcg catggcaaag gccagcgtag cccaactcga ggcgatgaag
     2281 gactacgaga tactgcgcgc gccattcgac gggacggtga ccgcgcgtta cgtcgatgtc
     2341 ggcgcactgg tgcaaagctc cgtcaccaac cagaccagca accaacctgt ggtgatggtc
     2401 gccgatatgt cgaaattgcg ggtcgacgtc tatgtcgagc agggcgatgt tccctacgtg
     2461 cacgtgggcg acgtcgccga tgtcgccgac gccgccgacg catcccgcca cgttcgggcg
     2521 cggatcgccc ggacatcgga tcaactcgat ccgcgcaccc gaacgctctt tgtcgaactc
     2581 gatgtggaca acagcgagaa cttcctggtg cccggcagct tcgcttacgt aacactgcat
     2641 gcaccggtag cgtcttaccc ggagatcccg attgccgcat tgatcacgcg tggcgccaag
     2701 acctttgtgg ccgatgtcag taaggacagc ctcgtgcatc tccaggcagt gaagatcgct
     2761 aataccgacg gcatcgtcgc gagtgttgcc gacggtgcaa aggtaggcga tcgcgtcgca
     2821 ttgaacctgc cggacgaggt gactgatggc ggccggatcc gccccgttga aaccagccac
     2881 tgacacgatc tacccggtca aggtccacac cgccattcgc gtcacgatgc gcgatggcgt
     2941 ggagctgaac gtacgcgtga cgcggcccga tgcacccggc cgctttcccg gcgtgatgga
     3001 atatcatccc taccggcgcc tgggagccgc attgccggac tatcgcgacg aatatccgcc
     3061 cgtggtgcca tatctcgccg agcgcggcta cgccgtggtg cagttcgacg tgcgtggcac
     3121 tggaagttcg tcgggctatt ccactgatat ctactcgccg gaggaacgcc aggacggcta
     3181 cgacatggtc gcctggattg ccgcacagcc ctggtgcacc ggcgccgtcg gcatgatcgg
     3241 caagtcctac ggcgccgtag tgcagtggca ggtggctgta cagcggcccc ccgccctcaa
     3301 agcaattgtc gtgcgctccg ccaatgacga cgtatatacc ggcttcacca accccggcgg
     3361 ctgcatccgt ccctggatgt tcgaatacta cgcgcccctg atgaacgcgc tgaactttgc
     3421 gccacccgac ccggccctgg tgggcgaccg ctgggcggcg atctgggcgg agcggctgga
     3481 gcacagtgca ccctggagcc tcggctacat ccgcaacacg ctgcacggcc cgtactggcg
     3541 cgaccagagc ctcgcacctg actacgaccg ggtggagtgc gccgtgttgc tgatcgaggg
     3601 ctgggccgat tggtacgcca ccgaggaact gcgtgccttc cagcgcttga acacgccgaa
     3661 gaaggtactg atcggcccct ggggccacta ctacgccgag gagcagcagg cgttccccgg
     3721 cccccgcatc gacgcgcgcc gcgagtacct gcactggttc gatcactggc ttaaggacat
     3781 cgacaacggc gtgatgtcgg agccccccgt caccgtattc gtacgtggct ggcaggcgcc
     3841 atcgctgctg tgcctcgaag acactggcgc gtggcggaac gaatcgacat ggccgcctga
     3901 gcgcctgcat cccaccaccc tctatttgtc cgaaggcggc atgctgaccg acaccgcagg
     3961 caacagcggc tccgacagct accagtaccg cccgtccgtc ggcatcacga ccggacgccg
     4021 gggcctgggg tccaccacgc cgtgggcaat gccgatcgac cagcgcctgg acgaggccca
     4081 ctcgctgctg ttcacgacgg agccattgcg ccaggacctg gagctgctcg gccaacctga
     4141 ggcactgctg cacatcgcat ccaccgccga agtcgcttac ttccacatga aactatgcga
     4201 tctcgctccc gacggcgcgt cccgcctgat ctgtgacggc gggttgctgg caacgcatcg
     4261 caattcgcac gagacccccg agaagctggc ccccggccag gtctacgcgc tgcgcatccc
     4321 gctgcgacac tgcgcctacg cgatccagac cggccaccgg ctgcgcatcg cgatagccag
     4381 cgcggaattc cagaatgcct ggccgaccgg ccagcacgcg gtcaacacga tcttccgcgg
     4441 cggccaccac gcctcccaca tcgtactgcc ggtcgcaccg cgcgatgcgc acccgccggc
     4501 accacctgaa ttcgccgcct cgccccacgc actgcccgca gcggactcgc tcgcaccccc
     4561 cggctacacg ttcgaacttg acctggtgaa cgacaccgtc acctgcgcac tgcgcgcgcc
     4621 cgagggtggc cgcaccagcc accactcgcg ctacaccgtg tcgaaccacg atcccgccca
     4681 caccacgatc atcggcagcg ccaagcacac cgcggtccat cccacgctcg atatccgcat
     4741 cgaggccacc tgccagacga ccagcgatgc cgacacctac agccacctct cgcaggtccg
     4801 catcaccgtc gatggcgcgc ctcacttcag caagagctgg tccgagagcg tgccgcggaa
     4861 gctgtcgtaa ggtcagtcaa ctccgtccga gcgcagccat cagctccgcc cactcgcgtc
     4921 ggttcaaccc gctgttcgct tggtccaccg cctcgtcagc cagcatccgc cgcaccgccg
     4981 tcagcatttg cgccgacagc gtcaccgccc ccagccggta atcgaggaac gcctgatgcg
     5041 ccaccggcac ccacgccgcg acactccgca gcatggcatc ggcatacacc ctgatctcgt
     5101 actgtgcatg cgcatcggcc cgcagcgaca ggaagtgtaa cagattgtag agatcgatct
     5161 tccagtacca ctgtgtatac gtgttcagcg tcaggttcat ccgtgccagt tcacgcgcca
     5221 gcccctgccg cgagggatcg cgcggcaccc ctgcctcatc ctcgttcagc atgtcggcat
     5281 aatggtcgta gcagcggatc gcatcctcgc gcagcatgtc gagcacccgg gctgcttcct
     5341 cacccgccag cacgtcgccg cgtccctgcc gattggccac cgactgcgcc gccagatgct
     5401 ccggcgccgg caggtagaat tcccggtcca ggatcgaata ccgcgcggag tactcgttca
     5461 cgttcgccgt ccgatgccgg atccattgcc gcgccacgaa tatcggcagt ttcacatgat
     5521 acttgatctc gcacatctcg aacggcgtgg agtgccggtg ccgcatcagg tagcggatca
     5581 gtcccgcatc ctccgacacc cgccgtgtgc cccgtccata cgacacgcgc gccgcctgca
     5641 cgatcgccga gtcgtcaccc atgtagtcga tcacccggac aaacccgtga tccagcaccg
     5701 ggatcgcctc gaacagcatc gcctccagcg ctgccacggt gggccgcgcg gtttccgccc
     5761 gttgggcgcg cgccgtcgcg atttcggcgg cctgctgttc ggtcagacgc atctgcgact
     5821 ccactttcat tcggcggggc gaatccctat attccctgcg ccagttcgct ggctatggcg
     5881 ataaacggtg cgtggaataa gcgtagtgga cccgggggca gtgcccggcg cctccaccat
     5941 tctggccgcg aagcggacaa tccccccgag gggtaatgtc agcttccagc cgtgcggggg
     6001 cgacacaggc tcgacacgcg tggtaaagac gcgtcttttg ctcggcatgg ttccgccgtt
     6061 atcgggccat tctacaagtg ccaatgacaa tgaggcgctc gctgtcgctg cataagcgat
     6121 aagcgcggtt cggggggcac cgggcaacag aagcccccca cttcacccct ccgcccctca
     6181 cgcaaccgtg cgcacggccg ggccaccgcc acagaatgtt ctgttgcttc ggtacacatt
     6241 acaggctcag aatgttgctc gctgctaggg cgattgcagc agtatagcgt gccagcagga
     6301 tttgctcccg tggtcacatg gtgatgcaat cgagtgtgac gccatcgtgg cacctcaagg
     6361 cccgccattc tctgctttcc ccacccaaaa gttacagtgt tcttgacttg aatagcgctt
     6421 gccaacggga gggaaacatg gacatcactc gccgcaccgc ggccgaattc ttcggtacat
     6481 tctggcttac cttcggaggc tgcggcgcgg ccgtcctcgc cgcaggattt ccgtcgcttg
     6541 gtatcggctt ccttggcgtg gcg
//
LOCUS       DATGEM010000545         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_541887, whole genome shotgun sequence.
ACCESSION   DATGEM010000545 DATGEM010000000
VERSION     DATGEM010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541887"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VKQ27_16645"
     CDS             complement(<1..220)
                     /locus_tag="VKQ27_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90611.1"
                     /translation="MVSESLRHYSSRLLLRSLMARLYRKIRVSDKAEAMERASIFSVS
                     RLRSSGLTFARPTAYSDRLTVRQPGELVL"
     gene            219..788
                     /locus_tag="VKQ27_16650"
     CDS             219..788
                     /locus_tag="VKQ27_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLY90612.1"
                     /translation="MRRQIGPLVCDRMTDPNVVEIMLNADGVLWEDRLGAGMSPIGEM
                     QPHTAEALIATVASMLRTTVTRENPIIECELPLDGSRFEAMIPPVVPGPVFTIRRKAS
                     RVFTFDDYVTAGIMQPGQRLALMQAVRDRSNILICGGTTPGKTTLTNAVIDQIVTTHS
                     ATPAGDHRGYGRAAMHGDELRSASGDRHR"
     gene            751..1125
                     /locus_tag="VKQ27_16655"
     CDS             751..1125
                     /locus_tag="VKQ27_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLY90613.1"
                     /translation="MNYVQLRATDTVDMRRLLKGTMRLRPDRIVVGEVRGGEALDMLK
                     AWNIGHPGGCCTVHANNARAAVIRIEQLIQEVSQSPMRRLIAEAIDLVVSIAMTSAGR
                     RVQEVLSVEVDRDDYVFTDRSN"
     gene            1127..1438
                     /locus_tag="VKQ27_16660"
     CDS             1127..1438
                     /locus_tag="VKQ27_16660"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HLY90614.1"
                     /translation="MRTRSFIAIAALNLILPLVPAHAAATGGSLPWDTPIQTLSNDIK
                     GPVAFTISLLGLVATGAALLFGGEINEFIRRIIMVVLVISLLVFAANILSTLFATGAV
                     V"
     gene            1435..1719
                     /gene="trbD"
                     /locus_tag="VKQ27_16665"
     CDS             1435..1719
                     /gene="trbD"
                     /locus_tag="VKQ27_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HLY90615.1"
                     /translation="MSRRRLPFQRALHRPNLLMGGERGPVLAAAVFGAGLAVTGQNLF
                     TLVVGAALWFASLPLLHWMAKADPMMTQVYRRHLRYRGYYPPRSRPYRRR"
     gene            1719..2807
                     /locus_tag="VKQ27_16670"
     CDS             1719..2807
                     /locus_tag="VKQ27_16670"
                     /inference="COORDINATES: protein motif:HMM:NF015118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90616.1"
                     /translation="MLALKHFRDTHAGLADLLNYAALIDYGVVQGKDGSLLIGWFYRG
                     QDSASSTDAEREWISGHVNRALSRLGSGWVTWQEAIKMPASGYPPVEASAFPDPVSRL
                     VDDERRRQFNRAGAHYDTAYALLALYTPPLRQKGRLIDMMYDDEPDEATEPASRILDG
                     LAKACAEIEDTLGDAVRIRRMGSIIITDGYGRDHLQDELVNYLQFAITGLHMGLNLPP
                     GGAYDSVLGGLEFWPGDTPRIGDKFIAWVSIEGFPPESYPGMLDVLDHLPVPYRWSNR
                     TIYLDQHEALTELHRYRRKWKQKIRGFWSQVFKTQGSVVNEDAVLMTSETEDAIARTS
                     SGLVGTGYFTSVVVLMDEDRAALMELLD"
     gene            3196..>4071
                     /locus_tag="VKQ27_16675"
     CDS             3196..>4071
                     /locus_tag="VKQ27_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90617.1"
                     /translation="MRGFMVELLAMLAAGMPLAGPAVAQSMMKTTLTVDLPNDVATLD
                     PQQQWDTDSYTVYRNIFDNLVTRDTSGTIVPQVATSWHYTDNTTLVFDIHKDITFQDG
                     TKLTPADVVFSVKRITNPAFKSAQLSQFDQITTAEVTGPDQVTLRTKTPYPVLLAQLV
                     KLSIVPQAYVERLGDVKFNQQPMGSGPYKLESRQNGVQSVLTANDHYWRGKPTFQTVI
                     FRVVPADATRVADLRAGRADIVRQITPDDADSLKSDNAVKVLAIPTERVGYLFINAQW
                     GPSKDVRVRRAIAMAI"
BASE COUNT          780 a         1262 c         1282 g          747 t
ORIGIN      
        1 gtagcacgag ctccccaggc tggcgaaccg tgagcctgtc cgaataggct gtcgggcggg
       61 caaaggtcag gccactcgag cggagacggc tgacagaaaa aatgcttgcg cgctccatag
      121 cttcggcttt atcagagaca cgaattttcc gatataaacg agccatgagc gaccgcagca
      181 gcaatcgaga tgaatagtgc cgcaggcttt ccgagaccat gcgtcgccag atcggtccgc
      241 tggtctgcga tcggatgacg gatccgaacg tcgtcgagat catgctcaat gccgatggcg
      301 ttctgtggga ggaccggctc ggtgcgggga tgtcgccgat cggcgagatg cagccgcata
      361 cagccgaggc gctgatcgca accgtcgcgt cgatgctgcg cactacggtc acgcgcgaga
      421 acccgatcat cgaatgcgaa ttgccgctcg atggctctcg cttcgaggca atgatcccgc
      481 ctgtggtgcc gggcccggtc ttcaccatcc gccggaaggc ttcgcgcgtc tttaccttcg
      541 atgactatgt gaccgccggg atcatgcagc caggccagcg gttagcgctg atgcaggccg
      601 tgcgcgaccg atcgaacatc ctgatctgcg gcggcaccac gccgggcaag acgacgctga
      661 ccaatgccgt gatcgaccag attgtcacca cccattccgc aacaccggct ggtgatcata
      721 gaggatacgg ccgagctgca atgcacggcg atgaactacg ttcagcttcg ggcgaccgac
      781 accgttgata tgcggcgact gctcaagggc accatgcgtc tgcggcccga caggatcgtc
      841 gtcggtgagg ttcggggcgg tgaagccctc gacatgctca aagcctggaa catcggccac
      901 cccggtgggt gctgcacggt ccacgccaac aatgcgcgtg ccgcggtgat ccggatcgag
      961 caattgatcc aggaggtcag ccagagcccg atgcggcgcc tgatcgccga ggcgatcgac
     1021 cttgtcgtgt cgatcgccat gacgtcggcg ggccggcgcg tgcaggaggt cctgtcggtc
     1081 gaagtcgacc gggacgatta cgttttcaca gaccggagca actgaattga ggacacgttc
     1141 cttcatcgcg atcgccgcgc tcaacctcat cctgccgctg gtccctgccc atgctgcggc
     1201 aaccggcggc tcgttgccgt gggacacgcc gattcaaacc ctgagcaatg acatcaaggg
     1261 gcccgtcgcc tttaccatct cgctgctcgg tcttgtggcg acgggtgctg ctctgttgtt
     1321 cggcggcgag atcaacgaat tcatccgccg catcatcatg gtcgtgctgg tgatctcgct
     1381 gctggtgttc gcggcaaaca ttctctcgac gctgttcgcg acgggagctg tcgtttgagc
     1441 cggcgccgcc tgcccttcca gcgtgccctg caccggccga acctgctcat gggcggcgag
     1501 cgtggcccgg tacttgccgc cgcagtgttt ggtgccggcc tggccgtgac cggacagaac
     1561 ctgttcacgc tggtcgtcgg tgccgccctg tggttcgcat cgctgccgct gctgcactgg
     1621 atggccaagg cggatccgat gatgacgcag gtctaccggc gccaccttcg ctatcgcggc
     1681 tactacccgc cgcgctctcg tccatatcgg aggagatgat gctcgcactc aagcatttcc
     1741 gcgacaccca tgccggcctc gccgacctgc tgaattacgc cgcgctgatc gattatggcg
     1801 tggttcaggg taaggacggc agcctgctga tcggctggtt ctaccgaggc caggacagtg
     1861 ccagcagcac cgatgccgag cgcgagtgga tatcggggca cgtcaatcgc gcgctgtccc
     1921 ggctcggcag cggatgggtc acctggcagg aagcgatcaa gatgccggcg tcgggttatc
     1981 cgccggtcga agcgtcggcc tttcccgacc cagtctcgcg cctggtcgat gatgagcgcc
     2041 gtcggcagtt caaccgagcc ggcgcccact acgacaccgc gtatgcgctg ctcgccctct
     2101 acacgcctcc gctgcggcaa aagggccggc tgatcgacat gatgtatgac gacgaaccgg
     2161 acgaggctac ggaaccggcg tcgcgcatcc ttgacggcct agcgaaggct tgcgcggaga
     2221 ttgaagatac cctcggcgat gccgtgcgca ttcgccgcat gggcagcatc atcatcaccg
     2281 atggctacgg ccgcgaccat ttgcaggacg agctggtcaa ctatctgcag ttcgcgatca
     2341 ccggcctgca catgggtctg aacctgccac caggcggcgc ctacgactcg gtgcttggcg
     2401 ggcttgagtt ctggccgggc gacacgccaa ggatcggtga caagttcatc gcgtgggtga
     2461 gcatcgaggg cttcccgccc gagagctatc ccggaatgct cgatgtcctc gatcacctgc
     2521 cggtgccgta tcgctggtcc aatcgcacga tctatctcga tcagcatgag gcactgaccg
     2581 aactgcaccg ctaccgccgg aagtggaagc agaagattcg cggcttctgg tcgcaggtgt
     2641 tcaagaccca gggcagcgtg gtgaatgagg atgccgtgct gatgacctcc gagaccgagg
     2701 atgccatcgc gcgtaccagc tcggggctgg tcggcaccgg ctacttcacc tcggtcgtgg
     2761 tgttgatgga cgaggaccgg gctgcgttga tggaactact cgactgaatg accaatattg
     2821 gcgcagagcg gacgggtgta gtgcagcacg cccgaccacg tgaataagac agctggctga
     2881 tcgacgcagt gcgaacttga ggcggtgtct cgaccggtta gggctcgtgg cggagatgat
     2941 gcaaatggag accgccacaa tcctggaggt acggcaaccc aatcgaggct tgcgtgcgtg
     3001 tcgacggcaa gcagctgacg cagcgtccgg cggtgagccc atcacacgcg ctcgataatt
     3061 ggcgttccga tgctggaccg aaaagatcgt cgtccaccta ccagccgcta ggtcatcggc
     3121 aaacctaaca ctgttgcgcg cggtatcggc ggcattcaga attcatcctg atcatggtgg
     3181 gggaagagga acacgatgcg gggattcatg gttgagctac tggcaatgct ggccgccggc
     3241 atgcccctcg ccgggccggc ggtggcgcaa tcgatgatga agacaacgct gactgtcgat
     3301 ctgccgaacg acgtggcgac cttggaccca cagcagcagt gggacacgga cagctacacg
     3361 gtttaccgga acatcttcga taacctcgtc acccgggaca catccggcac aatcgtcccg
     3421 caggtggcga cctcctggca ttacaccgat aacaccacgc tcgtcttcga tatccacaag
     3481 gacatcacct ttcaggacgg tacgaagctg acgccggcgg acgtggtgtt cagcgtcaag
     3541 cgcatcacca atccggcgtt caagagcgca cagctcagcc agttcgacca gatcaccacg
     3601 gccgaggtga caggacccga ccaggtcacg ctgcgcacca agacacccta tccggtgctg
     3661 ctggcgcagc tcgtgaagct ttcgatcgtg ccgcaggcct atgtcgagcg gcttggtgat
     3721 gtgaagttca accagcagcc gatgggcagc ggtccttata aactcgagag ccggcagaac
     3781 ggcgtgcaat ccgtgctgac ggcaaatgat cactactggc gcggcaagcc aaccttccag
     3841 acggtgatct tccgcgtggt cccggctgac gcgacccgcg ttgccgatct gcgtgccggc
     3901 cgggcggata tcgttcgcca gatcacgccg gacgacgcag attcgctgaa gtccgacaac
     3961 gcagtgaagg tgcttgcgat accgaccgag cgcgtcggct atctgttcat caatgcccag
     4021 tggggtccgt caaaggacgt gcgggtgcgg cgtgccatcg ccatggcaat c
//
LOCUS       DATGEM010000546        18150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_832732, whole genome shotgun sequence.
ACCESSION   DATGEM010000546 DATGEM010000000
VERSION     DATGEM010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832732"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VKQ27_16680"
     CDS             complement(<1..1261)
                     /locus_tag="VKQ27_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HLY90618.1"
                     /translation="MDIGLGHVPITLESRFDDLDTPVLLSGIQALVRVLLEQSRLDRA
                     AGLNTGGFVSGYRGSPLGGLDQELWRRQKLLAERNIQFQPGVNEDLAATMLYGTQHLD
                     AFPGKRVDGVFGMWYGKGPGVDRAGDALHCGNFLGTSKHGGVLAVSGDDHGAHSSTYP
                     HQTEHVFEGVFIPVLNPASAADMLDLGLAGIALSRYCGLWVALKTTAETAEQAATVII
                     PTARHFATPDFELPPHGLNFDPTMRFPADRAELERRVVLERIPAALAWARANRLDRKL
                     FGSDDAPIGIVTVGRAHEDTMHALRMLCLKDHAQIALYKVAMTWPLETSGIRDFARGK
                     RALLVIEEKRSFVESQIRDALYNLPADQRPEVSGKATQSGEPLLSPLLELSPEIVATG
                     MAAFLRLAGLNVPDPPAHRMPDRPAGLL"
     gene            complement(1624..2271)
                     /gene="alkB"
                     /locus_tag="VKQ27_16685"
     CDS             complement(1624..2271)
                     /gene="alkB"
                     /locus_tag="VKQ27_16685"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HLY90619.1"
                     /translation="MGDLLSVLPVRRSSEPIGDGAAVLRGFAFADANALLGAVERIQA
                     AAPFRHLTTPGGHVMSVAMTNCGELGWVSDRRGYRYQSADPLSGRTWPEMPDLLRRLA
                     DAAAKAVGYSGFCPDACLINRYEPGARLTLHQDRDEQELGAPIVSVSLGLPAVFLFGG
                     MRRSDRPRRIRLEHGDVVVWGGATRLAFHGIEPLADGHHPLTGATRINLTFRKAG"
     gene            complement(2284..2742)
                     /locus_tag="VKQ27_16690"
     CDS             complement(2284..2742)
                     /locus_tag="VKQ27_16690"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HLY90620.1"
                     /translation="MSFRITGLPSEPFANLFSLSDDELRARGAVRRSAEGNSPCRISL
                     TDAQPGDELILVNYEHHPVDSPYRMRFAIYVRRGEEQFDAIDEIPEQLRIRTLAVRAF
                     DANAMMVGWELVDGQQVESAIERLLAIPSAAYLHIHFAAPGCYAARVERV"
     gene            2990..3634
                     /locus_tag="VKQ27_16695"
     CDS             2990..3634
                     /locus_tag="VKQ27_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90621.1"
                     /translation="MTIDAELLNSRLQHVYWIGGGSGAGKSTTARRLAARYGMQLYST
                     DDVMPDHVRRCDPEDSPCLTAFRRMDMDERWVNRSPEAMLETFHWFRGEGFGLILEDL
                     LKLPSGERIIVEGFRLLPKLVQPLLRQPHQGIWLIPTPQFRLSAFETRGSLWTIAETT
                     SDPRRALDNLLRRDHMFTERLRCDAAEAGVPLVEVDGTLSEDTLEAHVARQFIL"
     gene            3745..4542
                     /locus_tag="VKQ27_16700"
     CDS             3745..4542
                     /locus_tag="VKQ27_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90622.1"
                     /translation="MVLGTGILSDEQIDQFIQDGFAKLERAFPREIADEARAILWKNT
                     GCDPDVPSTWVKPVIRLGDHGQEPFRKAVNTQLLHQAFDQLVGRGRWVPRISLGTFPI
                     RFPSPDDPGDTGWHIDVSYGLENSDFMEWRANISAKGRALLMLFLFSDVGELDAPTRI
                     RAGSHHDVARRLAPAGEEGLSLTDLAATGFRESAQRPEVLATGAAGTVHLCHPFLVHA
                     AQPHRGQLPRFIAQPPLLPREPLKLDRGDGAYSPIEAAIRLALCGAA"
     gene            4951..6534
                     /locus_tag="VKQ27_16705"
     CDS             4951..6534
                     /locus_tag="VKQ27_16705"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HLY90623.1"
                     /translation="MSIEHPSFGKLDDNRVIIFDTTLRDGEQSPGFSMNLNEKMRMAE
                     ALTELGIDVMEAGFPIASPGDFESVKAIAESVGQRDDSPVICGLARTGREDVSRAGDA
                     VRAAKRKRIHVFIATSALHMKYKLRMEPEEVLAASAAAVEQARGYTDDVEWSAEDGTR
                     TDPDFLCRCVETAIKAGATTINIPDTVGYALPEDMTRIFTMLLERVPGAEKIIFSTHN
                     HNDLGLAVANTLAAIRAGVRQVESTINGIGERAGNASLEEAIMAIRTRNDAIPYKNNV
                     QTTNLLRVSKLLSTITGFDVQPNKAIVGRNAFAHEAGIHQDGVLKNAQTYEIMTPESV
                     GWAKNSLVLGKHSGRAAFRDKLSTLGYRIGDNQLNDAFRRFKDLTDRKKVVFDEDIVA
                     LVDDEVVRDHERIRFVSLDLRAGSKAPPRAEMELEVDGVVKSATATGDGPVDATFKAI
                     QEIFPHTANLVLFSVGAVTEGTDAQAKTTVRLEENGKMVDGQGADTDTIVASARAYVH
                     ALNKLLVKRVRTEPQALSA"
     gene            complement(6539..6754)
                     /locus_tag="VKQ27_16710"
     CDS             complement(6539..6754)
                     /locus_tag="VKQ27_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90624.1"
                     /translation="MRNVLLLLAGLGLAGCGASPASLGITGPAPPPPPPKVDDSTIMN
                     PGIPDPGSGYGPSVGPSPTAGRYYNYN"
     gene            complement(6751..8955)
                     /locus_tag="VKQ27_16715"
     CDS             complement(6751..8955)
                     /locus_tag="VKQ27_16715"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HLY90625.1"
                     /translation="MRTQNRSFRLSAALAIGSLLLAQMPPPAAAQPASPPLATQQGDP
                     PTRVGRLARMTGTVSFHSQDETDWGPAVLNYPVTAGSSFWTQPGAQASIEVSASRVVM
                     APQTELDVSNLTDTAFEGTVPQGEVYLHVQATTPDETYAMQTPRGLVTLASPGRYGIA
                     AGDTQDPTLVTVIQGSAHIEGPGVTMDVAANQTAAVTGADSFQASVAPAQPDAFLTAM
                     LNAERPPVAPPPPVVASMPGGEDLSAYGSWSDTSDYGQVWYPQVAPDWVPYREGQWAY
                     VEPWGWTWVDSDPWGFAPFHYGRWVEIGGRWGWVPGAPVAGPPVYAPALVTFFGVGAV
                     VGIGIGAALASGRVGWCPLGPREAYQPWYRASPRYIRDVNVRHVTNFTTINRNVTVNN
                     FFNRRAATVVPTSAMTDSRPVARFAQRVDPAQLAQARPLVGAPPVHPVASTIGVTPRV
                     ARQFNLPASTAAARPVAPGPAFRATPATAHPVAPPLHNPGQNAVPATAAIHPAVPSGT
                     PMLRPPAAPGHVGPPPIEHTSPVVAPGAVPHTGQLPNAVEHPAAPVQPAVPQAHPVPG
                     AIQPQHVAPVTPIVPQAHPVPGAPAAIQPQHVAPMTAQPAMPQVHPTPAAPGAIQPQH
                     FAPMPQAPVPHAPPAAPAPVIVRSPPPAVLRAPEPQMHAAPAMPAPPPVFHPAPPPPV
                     MHAAPPPQMPQPAPPPPMVHAAPPPAAVHAPPPQPAPRPAPAQGEHKRPGEP"
     gene            9130..9375
                     /locus_tag="VKQ27_16720"
     CDS             9130..9375
                     /locus_tag="VKQ27_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90626.1"
                     /translation="MVCSSRLRRLMPLGALALVLALGGCYAYPAYPGYGYYGGGYYSG
                     GYYPQPYYGGGVVAFGGGWGGWHGHEGWHDHDGGWHH"
     gene            complement(9382..10554)
                     /locus_tag="VKQ27_16725"
     CDS             complement(9382..10554)
                     /locus_tag="VKQ27_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLY90627.1"
                     /translation="MTTESQIAAWLATQQDAMIALLREMVDIDSGSYNKPGIDAVGAV
                     VQRFMASQGIPVQVTAQQNHGDTVRAAVPWDGPAGNAGGNIVLMGHRDTVFPDGEVSR
                     RPFTIRDGVAYGPGVADMKAGLVMNCFVLAAFHKFGGSPAPLVGLFTGDEEIGSPEGR
                     AMIEAEARRARVVFNSEPGRVSGNVVTGRKGGVFSAFRITGKAAHSGGNFTEGVSAIE
                     ELARKIQAIHALTDLDRGITLNVGLVSGGQSVNTVAPWAEGQIDLRYVEPADRDDAMA
                     KIAAIIERSQVPGTKAELTIKGEFLPLVQTPTAKRLFEVYVQSAADTGFTTAGEFTGG
                     CADSGFTAGVGTPTICAVGPVGGKAHSPDEFLRIDSLVPRAQACARAILRLDQAGL"
     gene            10702..10956
                     /locus_tag="VKQ27_16730"
     CDS             10702..10956
                     /locus_tag="VKQ27_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90628.1"
                     /translation="MHRSIWIVVALSLAGCGGGSKSSTTVSVTCAGGVQLRGADSVDV
                     MGDLANGRPVIEFPDPVNPGKTGAIAIPQHDHCKVTADGG"
     gene            complement(10972..11772)
                     /locus_tag="VKQ27_16735"
     CDS             complement(10972..11772)
                     /locus_tag="VKQ27_16735"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY90629.1"
                     /translation="MRFDGKVALITGAGSGIGRATAVGFAQRGGAVAVADVNQETATA
                     VVTQITRAGGRAMAVVADLTDVTAIESMIADTLSAYGRIDVLHNNAFGVPSSIYKGRI
                     APVAEIDQQVWDYTLQIGLTAVMQATRAVLPLMQKQGGGAIVNTASISGLFADYGIAA
                     YNAMKAAVVNFTRVVAIEYAGDNIRCNCICPGAIDTPLLQRSLTTIQGFADATLAAIP
                     MGRLGTPDEMANVALFLASDLASYVTGSAFVADGGLTAKTGLPTRFRN"
     gene            complement(11839..12750)
                     /locus_tag="VKQ27_16740"
     CDS             complement(11839..12750)
                     /locus_tag="VKQ27_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90630.1"
                     /translation="MRTIRSLPSWVMLPAGVILLYGLAAQAAADDTRVAAMTRDEIAM
                     LVFIGGGALSPDEQREAGQIVEHAMATRPAFWTDADGKARIVLQRISAGDPITRADLR
                     ERLRAAAALHPAADPDLAKEAEIIDAHDPIVVFDPDHKVVVTQQTLLAWQQADRLMAD
                     RLQLAGPGSDFLISAAATVRASEAKWDEQTCDAYAHVARNLAAIQVYLTKDANTRKIA
                     GYFAKLRTALAQGPDATQSDRVAEYTAVRLLPAAYNVHEQRVARSGGAGAAAAMQLQL
                     NAMQLRQIHHTFMCAPGNYSGFCSTLP"
     gene            complement(12765..15239)
                     /locus_tag="VKQ27_16745"
     CDS             complement(12765..15239)
                     /locus_tag="VKQ27_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90631.1"
                     /translation="MSGVSGDGIGNGTRQVPEPVSTEVNLADWSAAIGSLRSDPAGSP
                     AHSPASVAAEPGIAAALSGTVERAPGELLGQVAEAVAPALSLAVLPGDAGLPLDASTT
                     LASNRAAQPLSGGSGPGAAEAATQAPVLASFETASTFGFRLPGDNAGQSAASPFLSLN
                     STDPGLSNGNPLLAWGLASVLGGPDRAPLWTAMGITPQELADRLQSAPWDVAAFQLGR
                     AVTARQYDALVALPGPLGALLRAGVDPRTALTLQAGWEAVMFGGRDPFAGRELTPLWQ
                     QFGLASHDELRALAAALQAGAAALPEAGQRPSVEAQRLLGASPILPPLAPQGSVAALI
                     ALPGAGFAGFHPASPGELGTSTAAVLIGAADGELLLARSTPVISARDATDASVLSAFG
                     NLHGNVAGPQDQPDTLRFGDVLRLHLGVAGETPFAVASWSPEAGFPPASLLPVLHAVL
                     NAAPAGLRAADILLPLYGPELSPSGTIALDTGATVAFGMAPPMGFTQGVPPSALPLVG
                     LQLRGSLAPSSGPAPRIVHEMRRPVPIDLTPLDSAVPLWRIDDTRILVDLGPGGSHEA
                     AQRAATLCQALTGDAPDLGLLQAGEGFSTWAGSPLWLALAGIPKPLAMGVLPEARPPR
                     VEISHTVEGEWLVSHAGDPVGAMQRRLHPRQAVDFEALLQDLLYAMPEEPETNGLDPR
                     AQTSLEAFRAARAWMAAATGLGDRDASLRAMEAWAGVARDCAAVLNCAGADSTVLSAA
                     HAALAGAEPDAGPALDRAGFLALMRRFLDRGGGALSRYGLHAVEAVRVGKIDGIGPAE
                     RTAIAACLGRLVGPLAGVSNPLFAVV"
     gene            complement(15260..16303)
                     /locus_tag="VKQ27_16750"
     CDS             complement(15260..16303)
                     /locus_tag="VKQ27_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLY90632.1"
                     /translation="MAKDTSEERKLPPSRKKLTDARKKGQVARSKDLVSGTALIGALG
                     FLMLAGGGMLAGATAMFDRAGDVAAGDFATGLRAMGPVLRAAALHMVVPLYLIVPLLV
                     VLSAMVMLRGIPFAVDPIAPRMENINPAEGFKRLFKLKSLIELLKSLIKMSLLGTAIV
                     LVLMGGLQALMLVPACGLNCAGNLFHALLTRLVATACLLFLVAGLIDVGLQRWLFVRD
                     QRMSVTEAKRERRDLDGDPLLRRERRRLLRDAARVAPLGLRRATLVLYDGQRSVVGVR
                     FKVNETPVPLVVCRATGERAEPMLAEAARLELATLEDPELAARLIRIMPGQIIPQDCY
                     RDVAVALTRAKLV"
     gene            complement(16307..17113)
                     /gene="sctT"
                     /locus_tag="VKQ27_16755"
     CDS             complement(16307..17113)
                     /gene="sctT"
                     /locus_tag="VKQ27_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HLY90633.1"
                     /translation="MDASALNALSALVRVPLTAFGIGFARMFGLLLILPMSTRLGLRG
                     LQRAGVAAALSCLIVPALVPQLDAQAIDTARLVLLIAKEACMGFMLGLLFAAPFWAAE
                     SAGEILDIQRGSRGALVPDPVSEEQGGITATLLVLTTIAIFLASDGMHLLINAMLQSY
                     RVWPALDLAPHFAPSAALQLLSLLDDVLRAGLLLAAPILGAMLLTELTLALISRFAPQ
                     LNVFDLALSVKGLVFVIGLPLYAVFLIGYLRTGLAPLLDLDAAFHRLAGN"
     gene            complement(17113..17382)
                     /gene="sctS"
                     /locus_tag="VKQ27_16760"
     CDS             complement(17113..17382)
                     /gene="sctS"
                     /locus_tag="VKQ27_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HLY90634.1"
                     /translation="MDRGELVHHLNGTLLLVLYLSAPVLGVAAAVGLVVGLLQAVTQI
                     QDQSLPQTLKLVAVLVTVALIGPLLARPLLNEATDVLNQFPALTR"
     gene            complement(17388..18047)
                     /gene="sctR"
                     /locus_tag="VKQ27_16765"
     CDS             complement(17388..18047)
                     /gene="sctR"
                     /locus_tag="VKQ27_16765"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HLY90635.1"
                     /translation="MNEPATALVPLILATAVVGLIPFLVITTTAFLKISIVLFLIRNA
                     LGTQQTPPTLVMYAIAIVVTLYVSAPLLSEIYARLTAPTADFNSASGISAVAHSVIDP
                     LRGYLVKFAAPGERDFLMSAAQRIWPPDFYRNGSRDDLMIVIPAFVISELTRAFEIGF
                     LLYLPFIAIDLIVSNILMAMGMVMVSPLVISTPFKIFLFVMVDGWTRLLHGLILSYAD
                     K"
BASE COUNT         3341 a         5929 c         5877 g         3003 t
ORIGIN      
        1 gcagcagccc cgccggccga tcaggcatac gatgcgcggg cggatctggc acattcagtc
       61 cggccaagcg caggaacgcc gccatgccgg tcgcgacgat ctcgggcgac aattccagca
      121 gcggcgacaa cagcggttcg cccgattgcg tggctttgcc actgacttcc ggcctctgat
      181 cggctggcag attatacagc gcatcgcgta tctgcgattc gacgaagctg cgcttctcct
      241 cgatcaccag cagtgcgcgc ttgcctctcg cgaagtcccg aatgccgctg gtttccagcg
      301 gccatgtcat cgccaccttg tagagcgcga tctgcgcatg atccttcagg cacagcatgc
      361 gcagcgcgtg catcgtgtcc tcgtgcgcgc ggccgacggt gacgatgccg attggcgcat
      421 cgtcgctgcc gaacagcttg cgatcgagcc ggttggcgcg tgcccaggcg agcgctgccg
      481 gaatgcgttc caacaccacg cggcgctcca gctcggcgcg atcggccggg aagcgcattg
      541 tgggatcgaa gttcagccca tgcggcggca gctcgaaatc cggtgtggcg aagtggcgcg
      601 cggtcgggat gatcacggtg gcggcctgct cggccgtttc cgccgtcgtc ttgagcgcaa
      661 cccacaaacc gcagtagcgc gacagcgcga taccggcgag gccgaggtcg agcatgtccg
      721 ccgccgaagc cggattcagc accgggatga acacgccctc gaacacatgc tcggtctgat
      781 gcggataagt tgacgaatgc gcgccatggt cgtcgcccga cacggcgagc acgccaccat
      841 gcttcgatgt gcccaggaaa ttgccgcagt gcagcgcgtc gcccgcgcga tcgacgccgg
      901 ggcctttgcc gtaccacatg ccaaacacgc catcgacgcg cttgccaggg aacgcatcga
      961 gatgctgcgt cccgtacagc attgtcgcag cgaggtcctc gttcaccccc ggctggaact
     1021 ggatattgcg ctcggccagc agcttctggc gacgccacag ttcctgatcc agtccgccca
     1081 gcggcgaacc gcgatagccg gaaacaaaac cgccggtgtt caaaccggcg gcgcgatcga
     1141 ggcgggattg ttccagcagg acgcgcacga gcgcctggat gccggaaagg agcaccggcg
     1201 tgtcgaggtc atcgaaccgg ctttccagcg tgatggggac gtggccgaga ccaatgtcca
     1261 tcctggcttc tctcccaagt atcccgggaa cttaaacgct ccgtcgcgaa cttgccatgt
     1321 gaggtgaacc cgcgcacgcg gagttggcga aattagagat gaagctgcgc agtgcgcagg
     1381 tgcgccgcca ccgtgccgcg agcagaagcg gaagcccggg taatattatt gcgcgcgccc
     1441 ggccagcttc cggagcgcac ggaatcgatt gattgtatgc gcctgcatcg acccaagctg
     1501 gcgagatcat gcgggagaca acgccgctga ctgaggctgg cgcaatgaag ccgatcgttg
     1561 tgtgatcgag gcggaagagg cgcgcgccaa gccggttgtc gatgtcggct gactgttgcg
     1621 ctgttatcct gccttgcgaa aggtgagatt gatgcgagtt gcccccgtca ggggatgatg
     1681 cccgtcagca agcggctcga taccgtgaaa ggccaggcgt gttgcgccgc cccagaccac
     1741 gacatcgccg tgttccagcc ggatacgccg aggcctgtcg ctccgccgca tgccaccgaa
     1801 caggaaaacc gccggcagcc ctagcgacac cgatacgatc ggagcgccga gctcctgttc
     1861 gtcgcggtcc tgatgcaggg taagccgcgc gcccggttcg tagcggttga tcaggcaggc
     1921 atccgggcag aagcccgaat accccacagc cttcgctgcc gcgtccgcga gccggcgcaa
     1981 cagatccggc atctccggcc aggttcgtcc gctgagcgga tcggccgact ggtagcgata
     2041 gccacgccgg tccgacaccc accccagctc gccgcaattc gtcatcgcga ccgacatcac
     2101 gtgtccgcca ggcgtcgtca ggtggcgaaa cggcgcggcc gcctggatcc gctcgaccgc
     2161 gcccagcagc gcgttggcgt cggcgaaagc gaagccacgc aggacggccg cgccgtcgcc
     2221 gatcggctcg ctgctgcgac gaacgggaag aacactcagg aggtcgccca cagccccccc
     2281 ttttcagacg cgctcgacgc gcgcggcata gcagccgggt gccgcgaaat ggatgtgcag
     2341 ataggcagcc gacgggatcg ccaacagacg ctcgatcgca gattcaacct gctgtccatc
     2401 caccaactcc caacccacca tcatcgcgtt agcgtcgaac gcgcggaccg ccagggtccg
     2461 gatgcgcagt tgctcaggga tctcatcgat cgcgtcgaac tgctcttcac cgcgccgcac
     2521 atagatggcg aagcgcatgc gatatggcga gtccacggga tgatgttcgt agttgacgag
     2581 gatcaactcg tcgcctggct gcgcgtcggt gaggctgatg cggcaggggg agttgccttc
     2641 cgcactgcgc cgcaccgcac cgcgcgcccg cagttcgtca tccgagaggc tgaacagatt
     2701 ggcgaagggt tcggatggga ggccggtaat gcggaagctc atcgttgtgc tccatgcgtt
     2761 gacagtgtgc acataaggca ttgccgaacc ccataccctc cgatgattgc gctggctccg
     2821 ctgcgtgcct gccccgatgc ggtgatggga cggttggagc gttggcttcg acccacctcg
     2881 gtggctcaac cagtgattct gggagccgac ctaccgctgt ctccaaaggt cgatggattg
     2941 aggacgtgtt ttgcgacatg cttcgccgcg ccgcgcttga gatagcgaaa tgacgataga
     3001 cgctgaattg ctcaacagcc gcttgcagca cgtatattgg atcggcggcg gaagtggcgc
     3061 cggaaagtcc acgaccgcgc gtcggctcgc cgctcgttat ggcatgcaac tctattcgac
     3121 ggacgatgtg atgccggatc atgtccggcg gtgcgatcca gaagatagtc catgcctcac
     3181 cgcattcagg cggatggaca tggatgaacg gtgggtgaat cgcagcccgg aagccatgct
     3241 tgagacattt cattggttca ggggcgaggg tttcggtctg atattggagg atttgctgaa
     3301 actccccagt ggagagcgga taatcgttga gggtttcaga ttgctgccaa agcttgtgca
     3361 gccgttgcta cgccagccgc accaaggcat ttggctgatt ccaacaccac aattccggtt
     3421 gtctgcgttt gagacgcgag gatcgttgtg gaccatagct gagacgacca gcgatccgcg
     3481 acgtgcccta gacaacctgc tcaggcgtga tcacatgttt accgaacgac ttcgatgcga
     3541 tgcggccgaa gctggcgtgc ccctggttga agtcgacggc accttgtctg aagacacgct
     3601 cgaagctcac gtggcgagac agttcatact gtaatcgatt ggtacatcgg caaaagatcg
     3661 cgtggcaaca atcagccaaa ttgatgcaga taatcgtcaa agctgcgcgc caaatcttga
     3721 ccagccaatc ttgcgagcat cccgatggtt cttggcacag gcatattgtc cgacgagcaa
     3781 atcgaccagt tcatccagga cgggttcgcc aagttggaac gcgcctttcc gcgagagata
     3841 gccgacgaag cacgtgccat actgtggaaa aatacaggct gcgatcccga cgttccatca
     3901 acttgggtga aacccgtcat caggctcggg gatcatggac aggagccgtt caggaaagca
     3961 gttaacacgc aactgctgca tcaagccttc gaccagctgg tgggacgcgg ccggtgggtt
     4021 ccgcgtataa gcctcggcac gtttcccatc cgctttccct caccggatga tcccggggac
     4081 accggttggc atatcgatgt gagctacggg ttggaaaatt ccgatttcat ggaatggcga
     4141 gccaatatca gcgccaaggg acgggcgctg ctcatgctgt tcctgttctc ggatgttggc
     4201 gaactggacg cacccacgcg aattcgcgcc ggatcacatc atgacgtggc ccgccgcttg
     4261 gctcctgccg gcgaagaggg gttatcgttg accgaccttg cagccaccgg atttcgggaa
     4321 tctgcacagc gtccggaagt gctggcgact ggcgcggccg gaacggttca tctgtgccat
     4381 ccgtttctcg tccatgcagc acagccgcat cgagggcagc tgccacgctt tatcgcgcag
     4441 cccccgttgc tgccccgcga gccgctgaag cttgaccggg gtgatggcgc ctactcaccc
     4501 atcgaggcag ccatcaggct cgcactctgt ggcgccgctt agctccgcgg ccccagagaa
     4561 caactcggac ctgtgtctca cgcggtcgcc atgcgtggtg aagtgcagat cgtctccttc
     4621 catgccggga aataaggtcc gcttccttgg cttggccgtc acagaataga ccgtcacgaa
     4681 gccagccgta ctcgacgtgc agcctgcccc caccacttgc ggttgccatc cctcgcggga
     4741 tgagttatgc acagtgccat gttctgtggc ctgcgcctcg cctgccgttc tgcccgcgcc
     4801 gttgcgcgtg gcggcgcatg cctgctggcc atcggcctcc tccttcgact tagccgcccc
     4861 tgagcgctgc gccgggtcgc taacacgccg gcttcgctca gggggacccc tgaggctaac
     4921 gtcgtgcaac cagtccaagg acccgccaca atgagcatcg agcatcccag cttcggcaag
     4981 ctggacgaca accgagtcat cattttcgac accacgctgc gcgatggcga gcaatcgccc
     5041 ggcttctcga tgaacctcaa cgagaagatg cgcatggccg aagcgctgac cgagctgggc
     5101 atcgacgtga tggaggccgg cttccccatc gcctcgcccg gcgacttcga aagcgtgaag
     5161 gcgatcgccg agagcgtcgg ccagcgcgat gattctcctg tgatctgcgg cctggcccgc
     5221 accggccgcg aggacgtgtc gcgtgccggc gatgccgtgc gtgcggcgaa gcgcaagcgt
     5281 atccacgtgt tcatcgccac cagcgcgctg cacatgaaat acaaactgcg catggagccc
     5341 gaggaagtgc tcgccgccag cgccgccgcg gtggaacaag cacgtggcta taccgacgat
     5401 gtcgaatggt cggccgagga cggcacccgc accgacccag atttcctttg ccgctgcgtg
     5461 gaaaccgcga tcaaggcagg cgccacgacg atcaacatcc ctgacaccgt cggctacgcc
     5521 ctgcccgaag acatgacgcg catcttcacc atgctgctcg agcgtgtgcc aggggcagag
     5581 aagatcatct tctccacgca caaccacaac gatcttggcc ttgccgtggc gaacacattg
     5641 gcagccatcc gtgccggcgt ccggcaggtg gagtccacca tcaacggcat cggcgaacgc
     5701 gccggcaacg cctcgctgga agaggcgatc atggcgatcc gcacgcgcaa cgacgcaatt
     5761 ccatacaaga acaacgtgca gaccaccaac ctgctgcgcg tgtcgaaact gctctccacc
     5821 atcaccggct tcgatgtgca gcccaacaag gccatcgttg gccgcaacgc cttcgcgcac
     5881 gaggcaggca tccaccagga cggcgttctg aagaacgcgc agacctacga gatcatgaca
     5941 ccggagagcg tcggctgggc gaagaactcc ctggtgctgg gcaagcactc gggccgcgcg
     6001 gcgttccgcg acaagctgag cacgctcggc tatcgcatcg gcgacaacca gctgaacgac
     6061 gcattccgcc gcttcaagga cctaaccgac cgcaagaaag ttgtgttcga cgaggacatt
     6121 gtcgcgctgg tggatgatga agtggtgcgc gaccacgagc gcatccgctt tgtgtcgctc
     6181 gacctgcgtg ccggttcgaa agcgccgccg cgggccgaga tggagttgga agtggatggc
     6241 gtcgtgaagt cagccactgc gacaggcgat gggccggtcg atgcgacctt caaggcgatc
     6301 caggagatct tcccgcacac tgcgaacctc gtgctgttct ctgtcggcgc ggtgaccgag
     6361 ggcacggacg ctcaggccaa gaccacggtg cgcctggaag agaacggcaa gatggtcgat
     6421 ggccaaggtg ccgataccga caccatcgtg gcctccgcgc gcgcttacgt gcatgcgctc
     6481 aacaagctgc tggtgaagcg ggtacgcacc gagccgcagg cgctttcggc ctgatcgctc
     6541 agttgtagtt gtagtagcgt ccggcggtcg ggctcggtcc aacactgggc ccgtacccgc
     6601 tgccgggatc cggaatgccc gggttcatga tggtgctgtc gtcgaccttt ggcggcggcg
     6661 gcggcggcgc gggcccggtt atgccgagcg aagcgggcga ggcgccacac ccggcaagac
     6721 ccagtccggc cagcagaagc aacacgttcc tcatggttcg ccaggcctct tgtgctcgcc
     6781 ctgcgccggt gctgggcgcg gcgcgggttg tggcggtggc gcatggaccg cggcgggcgg
     6841 cggagcagca tggaccatcg gtggtggggg ggccggttgc ggcatttgtg gtggcggtgc
     6901 cgcatgcatc accgggggtg gcggggcagg atggaacacc ggcggcggtg ccggcatcgc
     6961 tggcgccgca tgcatttgcg gttccggagc acggaggacg gccggcggcg gggaacggac
     7021 aattacgggc gcaggcgctg ctggcggagc atgcggtacc ggagcctgtg gcatcggagc
     7081 aaaatgctgc ggctggatcg ctccaggcgc ggctggcgtt gggtggacct gtggcatggc
     7141 aggctgagcc gtcatcgggg ccacatgttg cggctggatc gctgcaggtg cgcccggcac
     7201 cggatgggcc tgcggaacaa tcggtgtcac cggggcaaca tgctgcggtt ggatcgcccc
     7261 tggcaccggg tgggcctgtg gcacagcagg ctggaccggc gcggccgggt gctcaacggc
     7321 gttaggcaac tgtccggtgt gtggcaccgc gccaggggct acaactggcg aggtatgctc
     7381 gatgggcggc ggcccgacat gccccggcgc ggccggtggc cgcagcatcg gggtcccgga
     7441 cggcaccgcc ggatggatgg ccgccgtggc cggaacagcg ttctgtcccg gattgtgcaa
     7501 tggcggcgcg acagggtgtg ccgtcgccgg cgtggcgcga aatgctggcc ccggcgcgac
     7561 cggacgtgcg gcggcggtcg atgccggcag gttgaactgc cgcgccacgc gaggcgtcac
     7621 gccgatggtt gacgcaaccg gatgcaccgg cggcgccccg accagcggcc gcgcctgggc
     7681 aagctgcgcc gggtcaaccc gctgcgcgaa ccgtgcaaca gggcgcgagt cggtcatcgc
     7741 actcgtcggc accaccgtcg ctgccctgcg gttgaagaaa ttgttcaccg ttacgttccg
     7801 gttgatggtc gtgaagttgg tgacatggcg cacgttcaca tcgcgtatgt atctcgggct
     7861 tgcacgatac cacggctgat aggcttcgcg cggcccaagt gggcaccagc cgacacggcc
     7921 ggaagccagc gcggcgccga taccgatccc taccaccgcg ccaacgccaa agaaggtgac
     7981 cagcgcgggc gcatagaccg gcgggccggc gaccggggcg ccgggtaccc aaccccagcg
     8041 tccaccgatt tcgacccaac ggccgtagtg gaacggcgcg aacccccagg gatcgctgtc
     8101 cacccaggtc cagccccatg gctcgacata agcccactgt ccttcacgat acgggaccca
     8161 gtcaggggca acctgcggat accagacctg gccataatcg cttgtgtcag accagctgcc
     8221 gtaggccgag aggtcctcac cgcccggcat ggacgcgaca accggcggag gcggggcgac
     8281 gggtggacgc tctgcgttca gcatcgcggt aaggaaggca tcgggctgtg caggtgcaac
     8341 gcttgcctgg aagctgtcgg cgccagtgac cgccgctgtc tgattggcgg caacgtccat
     8401 cgtcacgccg gggccttcga tatgcgccga accttgaatg acggtcacca gcgtcgggtc
     8461 ctgcgtgtcg cctgcggcaa tgccatagcg cccgggcgag gcgagcgtca ccaacccgcg
     8521 cggtgtctgc atcgcgtatg tctcgtcggg tgtcgtcgcc tgaacatgca gatagacctc
     8581 cccctgaggc acggttccct cgaacgcggt gtcggtcagg ttggagacgt cgagctcggt
     8641 ctgcggcgcc atgacgacgc ggctggcgga aacttcgatc gatgcctgcg cgccaggctg
     8701 cgtccagaag gaactgccgg ccgtaacggg atagttgagc accgccggac cccagtcggt
     8761 ctcgtcttgg gagtggaacg acaccgtccc tgtcatccgt gcaagccggc cgacacgcgt
     8821 cggcggatcg ccctgctgtg ttgcaagcgg cggcgatgca ggttgtgcgg cggccggtgg
     8881 tggcatttgc gccaacagca agctaccgat cgccagcgcg gcggacagac ggaaagatcg
     8941 gttctgcgtg cgcatgcttg caggttcctc ggagcggtag tccaatcctc atatccgccg
     9001 ccaacatggc ggaatcgcga cgaggttcgt ggaggctgga gcggacacgg gattgccgcg
     9061 aggcggtcat taaactgccg cgatgcgagc gtttctgatg ggagaaaatt cagcttcagg
     9121 agtccgagaa tggtctgctc ttcacgattg cgccggctca tgccgctggg cgccctcgcc
     9181 ttggtgttgg cgttgggcgg ttgctatgcc tatccagcgt atcccggcta cggctactac
     9241 ggtggcggct actacagcgg tggctactat ccgcagccct attatggtgg cggcgtcgtg
     9301 gcatttggcg gcggctgggg cggctggcat gggcatgagg gttggcacga ccacgacggc
     9361 ggctggcacc actgacctga cctataatcc ggcctgatcc aatcgcagga tcgcccgcgc
     9421 gcaagcctgg gcgcgcggca cgaggctgtc gatccgcagg aactcgtctg ggctgtgcgc
     9481 cttgccgccg accggcccca ccgcgcatat cgtcggcgta ccgacaccgg cggtgaaacc
     9541 cgaatcggca cagccgccgg tgaattcgcc ggccgtcgtg aacccggtat ccgccgccga
     9601 ttgcacatag acctcgaaca gccgcttcgc tgtcggcgtc tgcacgagag gcaggaattc
     9661 gcccttgatg gtcagctcgg ccttggtgcc aggcacctgg ctgcgctcga tgatcgcggc
     9721 aatcttcgcc atcgcgtcat cgcgatcggc tggctccaca tagcgcaggt cgatctgacc
     9781 ttcggcccag ggagctaccg tattcacgct ctgcccgcca ctcaccagcc cgacattcag
     9841 cgtgatgccg cgatcgagat cggtcagcgc atggatcgcc tggatcttgc gcgcgagctc
     9901 ctcgatcgcg ctgacgccct cggtgaagtt gccgcctgaa tgcgccgcct tgccggtgat
     9961 ccggaatgcc gagaacacgc cgcccttgcg cccggtgacg acgttgcccg aaacgcggcc
    10021 cggttcgctg ttgaacacga cgcgggcgcg gcgtgcctcg gcttcgatca tggcgcggcc
    10081 ttccgggctg ccaatctcct cgtcgccggt gaacaggccg acgagcggcg cgggtgagcc
    10141 gccgaacttg tggaaggcgg ccagcacgaa gcagttcatc accagcccgg ccttcatatc
    10201 ggcaacgccc ggcccatagg cgacaccgtc gcggatggtg aacgggcggc gcgacacctc
    10261 gccatcaggg aacaccgtgt cgcgatggcc catgagcacg atgttgccgc cggcattgcc
    10321 ggccgggcca tcccacggca ccgcggcgcg cacggtatcg ccgtggttct gctgcgcggt
    10381 gacctgcacc ggaatccctt gcgacgccat gaagcgctgc accaccgcgc cgacggcatc
    10441 gatgccgggc ttgttgtagc tgccgctgtc gatgtccacc atctcgcgca gcagcgcaat
    10501 catagcgtcc tgctgggtgg cgagccaggc ggcgatctgc gattcggtgg tcatgcgtgc
    10561 tcccgtgggt ttggcgggtg gagcgaagca agccgtggcg gatcgtgcaa tgctggtatc
    10621 gcttgcaacg cccgcgcgcg tgcctgctag gtttagggca gacctacctc gccctgacct
    10681 gcgaacaata aaggaggctc catgcatcgt tccatctgga tcgttgtcgc cctgtcactc
    10741 gctggttgtg gaggcggaag caaatcgtcg acgacggtct cggtcacctg cgcaggcggt
    10801 gttcagttga gaggagcaga ctccgttgac gtcatgggcg acctggcaaa cgggcgaccg
    10861 gtcatagagt tccccgatcc agtgaacccc ggcaaaaccg gcgccatcgc gataccgcag
    10921 catgatcact gcaaggtcac cgcggacggc ggctaagtca ggctgcgccg ctcagttgcg
    10981 gaaccgtgtc ggcagcccgg tcttcgcggt cagcccacca tctgcgacga atgcggaacc
    11041 cgtgacatag gacgccagat cggatgccag gaacaaggcg acgttcgcca tctcgtcggg
    11101 cgtgccaagc cggcccatcg ggatcgccgc cagcgtcgcg tcggcaaagc cctggatcgt
    11161 ggtgagcgag cgctgcagca gcggcgtatc gatcgcgcca gggcagatgc agttgcagcg
    11221 gatgttgtcg cccgcatatt cgatggcgac gacgcgggtg aagttcacca ccgctgcctt
    11281 catcgcgttg taggcggcga tgccataatc ggcgaacagc cctgaaatcg acgccgtgtt
    11341 gacgatggct ccgccgccct gcttctgcat cagcggcaat acggcgcggg tcgcctgcat
    11401 cactgcggtc aacccgatct gcaacgtgta gtcccacacc tgctgatcga tctcggcgac
    11461 cggggcgatg cggcctttgt agatactgga tggcacgcca aacgcattgt tgtgcagcac
    11521 gtcgatccgt ccgtaagcgc tcaacgtatc agcgatcata gactcgatcg cagtcacgtc
    11581 ggtcagatcg gccacgaccg ccattgcgcg gccgcccgcc ctggtgatct gcgtcacgac
    11641 cgctgtggcg gtttcctggt tcacatctgc caccgcaacc gcgccgccgc gttgcgcaaa
    11701 cccgaccgcg gtagccctgc cgatgccaga gcccgcgccg gtgataagcg caaccttgcc
    11761 gtcgaaacgc atgacgcccc tccgtgttgt tctgatcact gtgccgatac agcgacgcgt
    11821 gcgcaaacgc gtcgtggctc aaggcagcgt gctgcagaac cccgaatagt tgcccggagc
    11881 gcacatgaag gtgtggtgga tctggcgcag ttgcatcgcg ttcagttgca actgcatcgc
    11941 ggccgccgca ccagcgccgc ctgatcgggc gacgcgttgc tcgtggacat tgtaggctgc
    12001 aggcaacagg cggacagccg tgtactccgc aacgcggtct gactgtgttg catctggccc
    12061 ctgcgccagc gccgtgcgca gcttcgcgaa atacccggcg atcttccgcg tatttgcatc
    12121 cttcgtgaga tagacctgga tcgccgcgag gttgcgcgcg acatgggcgt atgcgtcgca
    12181 cgtctgctcg tcccacttcg cctccgatgc gcggaccgtc gccgccgcac tgatgaggaa
    12241 gtccgacccc ggcccagcca actgcagccg atcagccatc aaccggtcgg cctgttgcca
    12301 cgcgagcagc gtttgctgcg tgacgacaac cttgtggtcc ggatcgaaca cgacgatcgg
    12361 atcatgcgcg tcaatgatct ctgcttcctt cgcaaggtcc ggatcggccg ccggatgcag
    12421 cgcggcggcg gctcgcaggc gctcccgcag atccgcacgc gtgattggat cgccagccga
    12481 aatgcgctgc aggacgatgc gagccttacc atcggcatcg gtccagaacg cagggcgcgt
    12541 cgccatggca tgctccacga tctgccccgc ctcgcgctgt tcgtccggag agagtgctcc
    12601 accgccaatg aagaccagca tcgcgatctc atcgcgcgtc atcgcggcga cacgcgtgtc
    12661 atccgcggcg gcctgcgctg cgaggccata aagcagtatc acgccggcag gaagcatcac
    12721 ccaactgggc aggcttcgga tcgtgcgcat cacggtatcc tccctcagac gaccgcgaac
    12781 agaggattgc tgacgccggc cagtggaccg accagtcgcc cgaggcacgc agcgatggca
    12841 gtcctttccg ctggcccgat gccatcgatc ttgccaacgc ggacagcttc gacagcatgc
    12901 agcccatagc gcgaaagagc gcccccgccg cgatcgagga accgccgcat cagcgccagg
    12961 aagcctgccc ggtccaacgc cggacctgca tccggttcag ccccggcaag cgcggcatgg
    13021 gctgccgaca acactgtgct atcggcgccc gcgcagttga ggacagcggc acaatctcgt
    13081 gccaccccgg cccacgcttc catggcgcgg agcgaggcgt cacgatctcc caggccggtt
    13141 gcggccgcca tccacgcgcg cgcggcacgg aacgcctcca ggctcgtttg ggcgcgcggg
    13201 tccagcccgt tggtttccgg ctcctccggc atcgcataga gcaagtcctg gagcagtgcc
    13261 tcaaagtcga ccgcctgccg cggatgcaaa cggcgctgca tggcaccgac tggatcgcca
    13321 gcgtggctga ccagccattc accttcaacc gtgtgcgaga tctcgacgcg cggcggtctg
    13381 gcctctggca gcacgcccat cgccaacggc ttcggaatac ccgccaacgc cagccacaag
    13441 ggcgagccgg cccaggtcga aaacccttct cccgcctgca gcagaccaag gtcgggagcg
    13501 tcgcctgtca gagcctggca cagcgtggcg gcgcgctgag ccgcttcgtg cgagccacct
    13561 gggcccaggt ccacgaggat ccgcgtatca tctatccgcc agaggggcac ggcggaatcc
    13621 agcggcgtca gatcgatcgg caccggccgc cgcatctcgt gcacgatccg cggcgctggg
    13681 cccgaactcg gcgcgagact gccacgcaac tgcaagccga ccagcggcaa agctgagggc
    13741 ggcacaccct gcgtgaagcc catcggcgga gccatgccga aagcgactgt cgcgcctgtg
    13801 tcgagcgcga tggtaccgct gggggacagc tccggtccgt acagcggcag caggatatcg
    13861 gctgctcgca gtcctgcagg cgcggcgttc agcacggcgt gcagcaccgg caacaagctt
    13921 gcaggaggga aacccgcctc cggcgaccag cttgcgacgg caaatggcgt ctcgcccgcc
    13981 acgccaagat gcaagcgcag tacatcacca aaccggaggg tatcgggctg atcctgtgga
    14041 cccgccacgt taccgtgcag gttcccgaat gccgacagga ccgatgcgtc ggttgcatcg
    14101 cgcgctgaga tgaccggcgt gctgcgcgcc agaagcagct cgccgtcggc cgcgccaatc
    14161 aatacggctg cggtcgatgt gcccagttca ccaggactgg ccggatgaaa cccggcgaag
    14221 cctgctccgg gcaacgcgat caacgcggcg acgcttccct gtggcgctag aggcgggagg
    14281 attggtgaag cgcccaacag tctctgcgcc tcgacgcttg gccgctggcc cgcctcgggc
    14341 aatgctgctg cgccagcttg aagtgcggcg gccagcgcgc gcaattcgtc gtggctcgca
    14401 aggccgaatt gctgccacag cggtgtcagt tcgcgcccgg cgaaagggtc gcggccaccg
    14461 aacatcaccg cttcccaacc ggcttgcagc gtcaatgcgg tgcgcggatc gacaccggcg
    14521 cgcagcaacg ctcctaacgg tccgggcaat gccacaagcg catcatactg tcgcgccgtc
    14581 accgcccggc cgagttgaaa tgctgccacg tcccacggcg ccgactgcag ccgatccgcc
    14641 agctcctgcg gcgtaatgcc catagcggtc cacagcggcg cgcgatcggg gccgcccagc
    14701 acgctcgcga gtccccaggc gagcagcgga ttgccgttgc tgagccccgg atcggtgctg
    14761 ttcaggctga gaaacgggga ggcagccgac tggccagcat tatcgcctgg cagccgaaat
    14821 ccgaaggtcg aagcggtctc gaagctagcc agcaccggag cctgggttgc ggcctcagcg
    14881 gcgccaggac ccgagccgcc agacaacggt tgtgctgccc ggttcgacgc aagcgtggtg
    14941 ctcgcgtcga gcggcaagcc ggcatctccg ggcaacaccg caagcgacag cgcgggcgcc
    15001 acggcctcgg cgacctggcc gagcagctcg cccggcgcgc gttcgacggt tcctgacagc
    15061 gcagccgcaa taccgggttc cgctgcaacc gaggccgggg aatgggcagg cgacccagcc
    15121 ggatccgatc gcagactgcc gatcgccgca ctccaatcag cgaggttcac ttccgtcgat
    15181 accggctccg gtacctgccg cgttccgttg ccgattccat ctccgctgac acccgacatc
    15241 gttcagcgct ccgtcatgct cacaccagct tggccctggt cagcgccacg gccacatcgc
    15301 gataacaatc ctgcgggatg atctgtcccg gcattatgcg gatcaggcga gcagccagtt
    15361 ccggatcctc cagcgtcgcg agttcgagcc tcgccgcttc ggccagcata ggttcggcgc
    15421 gctcaccggt ggcgcggcat acaaccagcg gcactggcgt ctcgttcacc ttgaagcgca
    15481 cgccaacgac agatcgttgc ccatcgtaca acaccagtgt cgcgcgccgc aggccaagcg
    15541 gtgcgacgcg tgctgcgtcg cgcagcagcc gacggcgttc gcggcgcagc agcgggtcac
    15601 catcgagatc cctgcgctca cgtttcgcct cggtgacgct catgcgctgg tcgcgcacga
    15661 acagccagcg ctgtaagccg acgtcgatca gtccggcgac caggaacagc aggcacgccg
    15721 tggccaccag ccgggtcagc aaggcgtgga agaggttgcc ggcgcagttc agtccgcacg
    15781 ccgggaccag catcagggcc tgcaggccac ccatcaggac cagcacgatc gcggtgccga
    15841 gcagcgacat tttgatcagc gacttcagca gttcgatcag cgacttcagc ttgaacagcc
    15901 gcttgaaacc ttccgccgga ttgatgttct ccatgcgcgg cgcgatcgga tcgaccgcga
    15961 acgggatgcc tcgcagcatg accatggcgc tcagcaccac cagcagcggc acgatcagat
    16021 acaacggcac caccatgtgg agcgcggccg cacgcagcac cggccccatg gcgcgcaggc
    16081 cggtggcgaa gtcgccggcg gcaacatcgc ccgcgcgatc gaacatggcc gtggcgccgg
    16141 cgagcatgcc gccaccggcc agcatcagga aacccagtgc accgatcaag gccgtgccgg
    16201 agaccagatc cttgctgcgc gccacctgcc ccttcttgcg cgcgtcagtg agcttcttgc
    16261 gggaaggcgg gagtttgcgc tcttcgctgg tgtctttcgc catcgctcaa ttccccgcca
    16321 ggcgatggaa tgccgcgtcc agatcaagca aaggcgccaa tcccgtacgc aggtagccaa
    16381 tcaggaacac ggcatagagc ggcaggccga tgacgaagac caggcccttc accgacaatg
    16441 cgagatcgaa cacgttgagc tgtggcgcga agcggctgat cagcgccagg gtcagttcgg
    16501 tcagcagcat ggcgcccagg attggcgcgg ccagcagcag gcctgcacgc agcacatcat
    16561 cgagcagcga cagcagttgc agcgcggcgg aaggtgcgaa gtgcggggca aggtcgagcg
    16621 ctggccagac gcgatagctt tgcaacatcg cgttgatcag caggtgcatg ccatccgagg
    16681 ccaggaaaat ggcaattgtg gtcaggacca gcaatgttgc ggtgatgcca ccctgttctt
    16741 cactgaccgg atctggcacc agggcgccac gcgaaccgcg ctggatgtcg aggatttcgc
    16801 cggcactttc cgctgcccag aacggcgccg cgaacaagag cccgagcatg aagcccatgc
    16861 aggcttcctt cgctatcagc agcacgaggc gcgccgtgtc gattgcctgt gcgtccagtt
    16921 gcggaaccag agcaggcacg atcaggcaac tgagcgcggc agcgacgccg gcgcgttgca
    16981 gtccgcgcag accgagcctc gttgacatcg ggaggatcag cagcagaccg aacatgcgtg
    17041 cgaagccgat gccgaatgcc gtcaacggga cgcgaaccaa ggcggacaga gcgttgagtg
    17101 cgctggcatc catcagcggg tcagcgcggg gaattggttc agcacatcgg tcgcctcgtt
    17161 cagcagcggc cgcgcgagga gaggaccgat cagcgcgacg gtgacgagca cggcgaccag
    17221 cttcaaggtc tgcggcaggg attggtcctg tatctgcgtg accgcctgca gcaggccaac
    17281 cacaagtccg acggctgcgg cgacaccgag tacgggcgcc gacagataca gcaccagcag
    17341 cagtgtgccg ttcagatgat gcacgagttc gccgcggtcc atgagcttca cttgtccgcg
    17401 tagctgagga tcaggccgtg cagcagccgg gtccagccat cgaccatgac gaacaggaag
    17461 atcttgaacg gcgttgagat caccagcggc gacaccatca ccatgcccat cgccatcagg
    17521 atgttggaga cgatcagatc gatggcgata aacggcaggt agagcaggaa gccgatctcg
    17581 aacgcacgcg tcagttccga gatgacaaag gccgggatta cgatcatcag atcgtcacgg
    17641 ctgccattgc ggtagaagtc gggtggccag atgcgctgcg cggccgacat caggaagtcg
    17701 cgctcccctg gcgcggcgaa cttgaccaga tagcctcgca gcggatcgat caccgaatgc
    17761 gccacagcac tgatgccgga tgcgctgttg aagtccgcgg tcggtgcggt cagccgcgcg
    17821 tagatctcgc tcagcagcgg cgcggacacg tagagcgtca ccacaatggc aatcgcgtac
    17881 atcaccagcg tcggcggtgt ctgctgcgtg cccagcgcat tgcgtatcag gaacagcacg
    17941 atcgatatct tcaggaacgc cgtggttgtg atgacgagga aagggatcag cccgaccact
    18001 gctgttgcca ggatcagcgg caccaacgcg gtggccggtt cgttcattcg cggccgatcc
    18061 aaagcacgcg aacgccgagg gctgaaccga ttgcgacgat ctcgccgcgg ccgatgcgcc
    18121 ggcccgagga cacgatgtcc acggcctgtt
//
LOCUS       DATGEM010000547         7050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_376126, whole genome shotgun sequence.
ACCESSION   DATGEM010000547 DATGEM010000000
VERSION     DATGEM010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376126"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..757)
                     /locus_tag="VKQ27_16770"
     CDS             complement(125..757)
                     /locus_tag="VKQ27_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90636.1"
                     /translation="MLKELNLLRYGSILQDIAVWMAALRKKAEPLHRKLLQNVANSSG
                     ELQIVRRLAQARRVGGRVEHYEAVRFAALRGAGALSLGPLPRQNLDIAAERLQTVVTT
                     IRTKSFSALSDKDQKHLITVVNQVSASLSETVNAIESALQFFERDNIGAIARWIEAQP
                     EIGGKLKPARNGWRWIDDRVDHVFEVKHLQPRTLLPLLPAFQRALRGAGA"
     gene            1436..1801
                     /locus_tag="VKQ27_16775"
     CDS             1436..1801
                     /locus_tag="VKQ27_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90637.1"
                     /translation="MSNLNKIADRCEAALGPDRDIDCVIGVAIGRFIAEPPRYDGGDI
                     RYREGNSWPGQAGDMLVPAYSSRIDAASTLVQEGQDWTVGRKSGLSFALVDGHERRGM
                     TPALALSSAALAARAARST"
     gene            2026..2370
                     /locus_tag="VKQ27_16780"
     CDS             2026..2370
                     /locus_tag="VKQ27_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90638.1"
                     /translation="MSVIRSLLDAAFSEGATRIHVHPPAMLQQLQFRLADGLAHARQH
                     PNVEAVFDELDLVGKGWFEGNIVYRGRTIRYFQSIATTGPDLVLILEPFDQPIDFPTR
                     PDIVLDDASGSR"
     gene            2562..3167
                     /locus_tag="VKQ27_16785"
     CDS             2562..3167
                     /locus_tag="VKQ27_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90639.1"
                     /translation="MPAPEVVVEPVYVQPVLDLSKTQRELFAAVSSTADATEKAAQPI
                     GFNYTDVTYSVDVRNTVLTDRSTICVRPSRATVRFGISTNIIYVSNELPPNSCIFHEV
                     FDHEMVHVHIDHRLLREMLNRLQAVLRQALDAAGPIVASSEQDGVQRVTRLAQDVVDR
                     EMHAYAVERQQRHGELDTPEEYRRVTASCGSELFKYISLGH"
     gene            3588..4346
                     /locus_tag="VKQ27_16790"
     CDS             3588..4346
                     /locus_tag="VKQ27_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImuA family protein"
                     /protein_id="HLY90640.1"
                     /translation="MATVSAHVLEDLRVRVARIEQGDRSQRGTIPFGVPAIDRHLPGG
                     GLALGSLHEVAGGGLGAVHGAAATLFAAGILARLSGSVLWCLKDRDLFAPALAGAGLH
                     PDRVIYAEGSDEKMVLACLEEGLRHPGLAGVIGEVSNLTMTASRRLHLAAETSGVLGL
                     VVRRWRTAAAAVDFGQPTAAVTRWRVSALPSSPLPVPGVGRPRWMVELIRARAGQSAE
                     WELESCNEQGRLALPAVLADRSVQAAEGGRRAAG"
     gene            4261..5775
                     /locus_tag="VKQ27_16795"
     CDS             4261..5775
                     /locus_tag="VKQ27_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLY90641.1"
                     /translation="MSRVVSLFLPSWPTDRFRRLKGAVAPPADRPLVLAAHVGGRRVI
                     TSADQAAQASGIRPGLPLAQAQARFPDLLVEDADPVEDAAALERLALWALRYSPVVAM
                     DPPAGVLIDISGAAHLMGGELALLDDLIQRLGRSGIRARASAADTIGAAHAMARWAVG
                     PMVAGRGATMAAIGPLPIAALRLDLDTVDRLRALGFETIADLEATPRAPLALRFGSLP
                     GRRLDQALGRLNEALDPIRPPELIETRRAFVEPIGAAETIARYIGLLVEPLCVRLEEQ
                     GLGALSLDLTFHRVDNQVQAVRVGLAQPARDPKRLARLLRDQIEKVVPGFGIELMVLA
                     ASLTGPLGMRQVGTLSEVPAVDLSGLVDTLANRIGADRIYRRALVESELPERSVGRVP
                     ALAPATGTSWLAGWPRPTRLLAPPERVDTLALLPDLPPVSFTWRSVRRKVMRADGPER
                     LFGEWHRRDAEMASVRDYFVVEDEAGERFWLFRAGDGEDPAAGNLNWYLHGIFA"
     gene            complement(5865..6863)
                     /locus_tag="VKQ27_16800"
     CDS             complement(5865..6863)
                     /locus_tag="VKQ27_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90642.1"
                     /translation="MPLQRHMMLAALFLIGTSPALTAEIKDCDPTLVVETAKSNFADS
                     EKVAASRLITKDNYDNFKTEASGKALVDEVPVGGSFSDFRSHREKQLSSSSDSRDSAV
                     LRGELRTWLSIEGVKAYEQCLETVEKSSIGFHAWISNPNKDSLTLHMLWNMGTLTDVG
                     TLTDAYFSGVTQAPPVPRTWVPNATQGFPLTRTAADFKFVANMNGMTTEVFLPAYRPP
                     PIQPEPLSMAFLQGVWCANGRQFVQFSSVSLKAAQVDGMGGLGGFPQRGQAEISREGD
                     SWTMTTKLGGIGLASGWHRTSDTAIVLDYFEEPVGLGAAAHATRSSKTNTALKRCS"
BASE COUNT         1229 a         2206 c         2223 g         1392 t
ORIGIN      
        1 gaacgcggtg cgggccgctc ggcgtctagc gatcggccgc ctgtttggct gggcagtcgg
       61 tcggagcacg ggcgcgatcg gcgccatctc gaccaggtgt tcgagggacc gggagcgcgg
      121 cgccctaagc gccggcgccg cggagcgcac gctggaacgc tgggagtagt ggcaggagcg
      181 tgcgcggctg caggtgcttg acctcgaaga cgtggtcgac gcggtcgtcg atccagcgcc
      241 agccatttcg ggctggtttc agcttgccgc cgatctcagg ctgcgcctca atccaacggg
      301 cgatcgctcc gatattgtcg cgctcgaaga actgtagcgc cgactcgatg gcattgaccg
      361 tttccgacag gctcgcactg acctggttta cgacggtgat caaatgcttt tggtccttgt
      421 cggacagggc ggaaaagctc ttggtccgta tcgtggtcac caccgtctgc aggcgttctg
      481 ccgcgatgtc gagattctgg cgcggaagcg gtccgagcga cagggccccc gcgccgcgca
      541 aggctgcgaa ccgcacggcc tcgtagtgct cgacgcggcc gcccacccgg cgggcctggg
      601 ccaaccgccg gacgatctgc aactcgcccg agctattggc gacattctgc agcaacttac
      661 gatgcagggg ctcggctttc ttgcgtagcg ccgccatcca cacggcgatg tcctggagga
      721 tcgatccgta gcggagcagg ttgagttcct tcagcatcgg cgcaatgagc ggtgcgacca
      781 acacgaaccc cgtctcccgc gcgcgccgcc ggtcggcctc tcgcatgcgg gtgactcgct
      841 gcgcataatc tgtgcccttg taggtcgaat tcgcgcagcg ccagcccacc gaccgaagcc
      901 agccgtctgg ataccagccc aagacgccgt tcccaaattt ctcgtcgatg ccgcacgtcg
      961 cgcaatagca ctgcacgccc gatccgttga tgtcgaaccc gaacagcggc tgcgggatat
     1021 ccccggcagg cgcaggaccg gcgagatacc cggggaggcc ggcgggcgag ttggcagcta
     1081 gcagctgcgc aagaagccca tcggcctcga cgggagtagg gcagcgtttc catggtccgg
     1141 cggttgccga gtgagacacc ggggcgatcg ccatcgtgcg ctcctaagaa cggatagcga
     1201 acggtacaat atcttgtggg agcccgcaac gggcgacgct ctatcgtgcg acgccccacc
     1261 ccttactcgc gcgggcgtcg cacatgcgac ggcgcccgtc tccttggttg ccgcagaacc
     1321 ggtcatctct tggatatgag cggatggcgg gctgttccgt cctgtttcga tagttcttga
     1381 ttaagccacc gggccgcgaa tatgggcaaa cgaatttcca gaacgagagg gcctaatgtc
     1441 gaatctgaac aaaattgcag atcgctgtga ggcggccctg ggccccgacc gggacattga
     1501 ctgcgtgatt ggtgtcgcga ttggccggtt catcgccgaa ccgccgcgct atgatggtgg
     1561 agatattcgg tatcgcgaag gcaatagttg gcccgggcaa gccggtgaca tgctcgtgcc
     1621 cgcttactcc agccgcatcg atgcggcgag caccctcgtc caggaaggcc aagattggac
     1681 cgttggtcga aagtcgggcc tgagtttcgc ccttgttgat ggtcatgagc gccggggaat
     1741 gactcccgca ttggcgctga gttccgcggc acttgccgcg cgcgccgcca gatccacgta
     1801 gtgtggcgca gatggatagg cagcagcacg ccctaatcgt tgacatacac ggcaagatcc
     1861 agaacgccgc tggcgcgctg gtccgagcga ccgcgcccgg acggagcaat gggccgatcg
     1921 ggaacaattt ctgagatgct gcggatcgcg acttcgcggc ttgccgcgtt gcgcgaggcg
     1981 ctagcaaccg aggaagcgtc caacaagtga gccgcggagg gccgcatgag cgtaattaga
     2041 tccctactcg atgccgcatt ttccgaaggt gccacgcgga tacacgtcca cccgccggcc
     2101 atgctgcagc agctgcagtt ccggcttgct gatggccttg cgcatgcgcg tcagcacccg
     2161 aacgtcgagg ccgtcttcga cgaactggat ctggtcggca agggatggtt cgaggggaac
     2221 atcgtctatc gcggccggac aatccgctat ttccagtcga tcgcgacgac gggtccggat
     2281 ctggtgctga tcctcgagcc atttgaccag ccgatcgact ttccgacccg gccggacatc
     2341 gtgttggacg atgcatctgg cagtcgctaa ccaaggcaga ctggacggcc gcgcggcggt
     2401 cgaagacgaa ttttccggtt gcccatcccg acaatctgtc gatatggacc gccagagcaa
     2461 tgacttggtg cggtggtatg cgacgtttga ttcttctgtt cctggcaact tgcgtcttcg
     2521 cgtcgctccc tctccccgga cgggcccaga cgtgcccgcc gatgcccgcg ccggaggttg
     2581 tggtcgagcc tgtttacgtc cagccggttc tggatctgag caagacgcaa cgcgagctgt
     2641 ttgccgccgt ctccagcacg gcggatgcca cggaaaaggc ggcgcaaccc atcggtttca
     2701 actacaccga cgtcacctat agcgtggatg tgcggaacac ggtgctgacc gaccgatcga
     2761 caatatgcgt gagaccgtct agggcaacgg tccgcttcgg aatttcgacg aacattatct
     2821 acgtttcgaa cgaactaccg cccaacagct gcattttcca cgaagtgttc gaccatgaaa
     2881 tggttcatgt gcatatcgat catcggttgc ttcgcgaaat gctgaatcgg ctccaggcgg
     2941 ttttgcgcca ggcgctggat gccgccgggc caatcgttgc ttcttcggaa caggatggcg
     3001 tgcagagggt cacgagactg gctcaagatg tcgtcgatcg ggaaatgcat gcctacgcgg
     3061 tcgagcggca gcagcgccac ggggagttgg acacaccgga ggagtaccgg cgggtaacgg
     3121 cgagctgcgg atcggagttg ttcaaatata tttcgctcgg tcattgaccg gacggcgcgt
     3181 cggccgtcgc gtgagggcta cgaggccccc cgaccggtga cgctcttccc atcggtgccg
     3241 gcgttgccgt ttccaacccg ccaccacctc ccagcctgat cctcccgtct cctcgggaga
     3301 ctcattctac cgagggctgg ggaaacgatc cggccgtgcc aggtgcgaca cgggctctcg
     3361 gcgaattctc gttttctggt tcatggattc tcatttgaaa gttggcattt tcaatctatc
     3421 gcattgaaat taatcagttt cgattctcat tttcaggttc accgtccagg ccgtccgtca
     3481 tcgcatgtcc atcgggttgc aaacgggcat gacgctttcg cgattgactc tctggtgatt
     3541 cggaacaaaa taggaacatc gaaatctgat tgccacttgg gagaaacatg gcgaccgtct
     3601 ccgcccatgt tctagaggat ttgcgcgtcc gcgttgcccg catcgagcag ggtgatcggt
     3661 cgcagcgcgg gacgattccg ttcggcgttc cggcgattga ccgacatttg cccggcggcg
     3721 gcctcgcgct cggttcactc catgaggtcg ctggcggcgg ccttggcgcc gtccatggtg
     3781 ctgcggcgac actcttcgcc gccggcatcc tcgcccggtt atccggctcc gtcctctggt
     3841 gcctcaagga ccgcgatctc ttcgccccgg cgctcgccgg tgccggcctc catcccgacc
     3901 gggtgatcta tgccgagggc agtgacgaga aaatggtgct cgcttgcctc gaggagggtc
     3961 tgcgccatcc ggggctcgcc ggggttatcg gcgaagtctc gaacctcacc atgacggcct
     4021 cacggcgcct gcatcttgcc gctgaaacct cgggcgtttt gggtctcgtc gtccggcgct
     4081 ggcgcactgc ggcggccgcg gtcgatttcg gccaaccgac cgccgctgtg acgcgctggc
     4141 gggtcagcgc cctgccctcc tcgcccctgc ccgtgccggg tgtcggccgg ccgcgctgga
     4201 tggtcgagct cattcgggca cgcgccgggc aatccgcaga gtgggaattg gagtcctgta
     4261 atgagcaggg tcgtctcgct cttcctgccg tcctggccga ccgatcggtt caggcggctg
     4321 aagggggccg tcgcgccgcc ggctgaccgt ccgctcgtgc tggccgctca cgttggcggt
     4381 agacgcgtca tcacttcggc cgatcaggcg gcccaggcct cagggattcg gccaggactg
     4441 ccgctcgccc aagcccaggc gcgcttccct gatctgctgg tcgaggatgc cgatccggtc
     4501 gaggatgcgg cagccctcga acgcctggcg ctctgggccc ttcggtacag ccccgtcgtc
     4561 gcgatggacc cgcccgccgg cgttctgatc gacatcagcg gggccgcgca tctgatgggc
     4621 ggagaattgg cactcctgga tgatctcatc cagcggctcg gccgatccgg catccgagcc
     4681 cgtgcgtccg cggcggacac gatcggtgcc gctcacgcga tggcgcgctg ggcggtcggg
     4741 ccgatggttg ccggccgagg cgctaccatg gccgccatcg gaccgttgcc gatcgcagca
     4801 ctccgcctcg atctcgatac ggtcgaccgg ctgcgcgcgc tcgggttcga gacgatcgct
     4861 gatctcgagg cgacgccacg cgcaccgcta gcgctccggt tcggttccct gccaggccgc
     4921 cggctcgatc aggcgctggg tcgcctcaac gaagccctcg atccgatccg gccgcccgag
     4981 ctcatcgaga cgcgccgcgc cttcgtcgaa ccgatcggcg ccgctgagac tatcgctcgc
     5041 tacatcggcc tgctggtcga gccgctgtgc gtccgcctcg aagagcaggg tctgggagca
     5101 ctcagcctcg atctcacttt tcaccgggtc gacaaccagg tccaggcggt gagagtgggt
     5161 ctcgcccagc cggcgcgcga tccgaaacga ctggcgcggc tgctgcgcga tcagatcgag
     5221 aaagttgtgc cggggttcgg tatcgaattg atggtgctgg ccgcctcgct cacaggcccg
     5281 ctcggcatgc gccaggtggg gacattgagc gaggtgccgg ccgtcgatct gtctggcctg
     5341 gtcgacaccc tcgccaaccg gatcggcgcc gatcggatct atcggcgggc actcgtcgag
     5401 agcgagctgc ccgagcggtc ggtcggccgg gttccggctc tggcgccggc gactggaacg
     5461 agctggctgg ccggatggcc gcggccgaca aggctccttg cgccgcccga gcgtgtcgac
     5521 acgctggcgc tgctgcccga cctgccgccg gtgagcttca cctggcgtag cgtgcgccgg
     5581 aaggttatgc gcgccgacgg gccggagcgc cttttcggtg agtggcacag gcgggatgcg
     5641 gagatggcgt ccgtgcgcga ctatttcgtg gtcgaggatg aggccggtga gcggttctgg
     5701 ctgttccggg ccggggacgg cgaggatccg gcggcgggaa acctgaactg gtatctgcac
     5761 gggatcttcg cgtgacgacg atccgcactc taaagaaccg gtctgtcagc gctgcgtctc
     5821 gtcgataggg cgtggcgcca cgcccggatc aggctcgtct acgttcagga acatcgtttc
     5881 agtgccgtgt tcgttttgga agagcgggtg gcatgggcag cggcaccgag cccgaccggc
     5941 tcttcaaaat aatcgagaac gatcgcagta tcgctcgtcc ggtgccaacc agacgctaga
     6001 ccaatcccac ctaatttggt ggtcatcgtc caactatcgc cctcgcggct tatctcggct
     6061 tgtccgcgtt gcgggaaacc tccaagaccg cccattccgt cgacctgggc ggctttcagg
     6121 gaaaccgaac taaattgaac aaactgacgg ccgtttgcac accagacccc ctgaaggaat
     6181 gccatagata gaggctcagg ttgtatcggc ggcggccgat aagcagggag aaatacttcg
     6241 gtcgtcatgc cgttcatatt ggcgacaaat ttgaagtcag ccgccgtcct ggttagtggg
     6301 aaaccctgcg tagcattggg tacccacgtt cgaggaaccg gcggtgcctg cgtcacccca
     6361 gaaaaatagg cgtcggtgag agtaccaacg tccgtcaatg ttcccatgtt ccataacatg
     6421 tgtaaagtta agctatcttt attcggattt gatatccatg cgtgaaagcc aatactagat
     6481 ttttctacag tttcgagaca ctgttcatag gctttcaccc cctcaattga caaccaagtt
     6541 cgtagctccc cgcgaaggac cgccgaatcc ctactatcgc tagaggaaga gagttgcttc
     6601 tcccggtgag atctgaagtc gctgaaactt ccgcccaccg gcacctcgtc gactaacgcc
     6661 ttcccactcg cttccgtctt gaagttgtca taattatctt tggtaatcaa acgcgaagcg
     6721 gctacctttt ccgagtcggc gaaattcgat ttggctgtct cgactaccag tgtcggatca
     6781 caatctttaa tttccgcggt cagtgctgga gatgttccta ttaggaacaa tgctgcaagc
     6841 atcatatggc gctgtagtgg catggtcgtt cctccgatag gagaacccac ggatgatcac
     6901 tctacgccta tgcactggat ccgcctaact tgcaaagcaa tgcacggtcg ttaaatggac
     6961 tcccgccggc gatgacgggt tcgtccgctg gccgtccatc tctcattcgg aagcgagatc
     7021 agcggagaca accgctacgg cgatctcctc
//
LOCUS       DATGEM010000548        12339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1533873, whole genome shotgun sequence.
ACCESSION   DATGEM010000548 DATGEM010000000
VERSION     DATGEM010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533873"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VKQ27_16805"
     CDS             <1..674
                     /locus_tag="VKQ27_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY90643.1"
                     /translation="VPNSISRCPAQSWETPMSHRQMIHIIDDEEAVRHSLAIMLQVAG
                     YAVTAWPGGASFLREAAHVEPGCVLLDIRMPEMDGLEVQRTMQEHGIAWPVVVLTGHG
                     DVTTAVAAMKAGAIDFLEKPLAKSTLVTAIEAALQSFEGTDAARRRAALASQRIARLT
                     AREQEVLRGLANGLPNRNIAHELAISPRTVEIHRANLMAKLGVRTLSDALRLAFSAGL
                     DSPVN"
     gene            complement(706..900)
                     /locus_tag="VKQ27_16810"
     CDS             complement(706..900)
                     /locus_tag="VKQ27_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90644.1"
                     /translation="MTKSLKRFTITPHGDGYLLHLETGDGEVFELAATFEQLDLIAEE
                     IDRRLDADEDEMLPLGDRND"
     gene            complement(958..1185)
                     /locus_tag="VKQ27_16815"
     CDS             complement(958..1185)
                     /locus_tag="VKQ27_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90645.1"
                     /translation="MITQAQRIGFYREWSSRAARQALAAIHPSTRERCTHSAGVWSSI
                     ANALEAGDDGEVRALTHNLTFLRIGQLIPEG"
     gene            complement(1377..1607)
                     /locus_tag="VKQ27_16820"
     CDS             complement(1377..1607)
                     /locus_tag="VKQ27_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90646.1"
                     /translation="MADLASHPNVLRSWLEWEIDGRERRLILVVETDIEMRPEADDFD
                     EALMDEVRAAAIAEMRAVPSALHLIRIVPAHP"
     gene            complement(1600..1770)
                     /locus_tag="VKQ27_16825"
     CDS             complement(1600..1770)
                     /locus_tag="VKQ27_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90647.1"
                     /translation="MCTATEAIYLADREQASRAAAQDAGECCARIAHEKLATAYAVRR
                     EGARAIGPAGHG"
     gene            1945..2553
                     /locus_tag="VKQ27_16830"
     CDS             1945..2553
                     /locus_tag="VKQ27_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90648.1"
                     /translation="MPSEDSEIPHARSIGIAQAHRTLCNATRFLGSGLSDAGSTPIGG
                     RYRAKVIGNGLRELDRFLSVLLDEVALAAGWERADLQRLANLRNTANKLGVIHGWLGR
                     PTHDLPRLRALGRCRDCLFHCNGVVRRGDERRAASMTVGWPADARGDAAGVRLALGEN
                     LAVSPADLAWICAFYDRIAGDLVEDIRGVRSDFARTEHELSC"
     gene            2956..3108
                     /locus_tag="VKQ27_16835"
     CDS             2956..3108
                     /locus_tag="VKQ27_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90649.1"
                     /translation="MNFLPPTLPVAGAKPDKPFYPHGCPAPQFRPRSATLSPAEVRRI
                     VAELIG"
     gene            3143..3340
                     /locus_tag="VKQ27_16840"
     CDS             3143..3340
                     /locus_tag="VKQ27_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90650.1"
                     /translation="MSVTSDFYLARAAECAQAADAAGLDNVRDRFRRSEAAWLAMAGR
                     LQRGEALRDVAAAEKAARGTP"
     gene            3686..3892
                     /locus_tag="VKQ27_16845"
     CDS             3686..3892
                     /locus_tag="VKQ27_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLY90651.1"
                     /translation="MITGTVKFFNADKGYGFIAPEDGSGDAFVHISAVERAGMRTLDK
                     DQRISYELETDRRGKTSAVNLQQA"
     gene            complement(4044..4862)
                     /locus_tag="VKQ27_16850"
     CDS             complement(4044..4862)
                     /locus_tag="VKQ27_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLY90652.1"
                     /translation="MRLLAILVGAKSAPACLDAATLAARALDHASVEALHVIVDPDHM
                     IAASEEIAIQQFRVVEEGTAWQRAKATRSAFAAWKTEAGACGCAVQWKTLTGHEEEIV
                     CREAGAADVVMLVMAREANLDSGDALHAAIFRCDKPVLIIPREWRRGSRAGFDHVVVG
                     LSNGEAARHAIEGAGPWLRAAKRVSAIRIGDRDDATLGLSRLLIEAGVEPALHVVPKH
                     GSDLGAQIIGEAHAVGADLLVSGAYRHSEMVDWLLGGTTRHMLAAADLPLLLAH"
     gene            complement(4937..5398)
                     /locus_tag="VKQ27_16855"
     CDS             complement(4937..5398)
                     /locus_tag="VKQ27_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLY90653.1"
                     /translation="MARIEPTDPDAPKLADFLCFAIYSANLAYGRAYKPLLDDLGVTY
                     TQWITIVALWEHDNETVSSLGEKLFLESNTLTPMLKKLEGMGYIRRQRDPADERQVRV
                     TLTETGRELRERGLQRTLVKATGLPADEFGLVQNSVVRVRDNLLKHTRDQN"
     gene            5572..5988
                     /locus_tag="VKQ27_16860"
     CDS             5572..5988
                     /locus_tag="VKQ27_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HLY90654.1"
                     /translation="MTKIENLLYTAKTHTTGGRDGHARSSDGLLDVRLSQPGGPGAGT
                     NPEQLFAAGWSACFIGAMGHVARQQKLALPADLSVDAEVDLGTADGGYLLQARLNVSL
                     PGLDREVARALVDAAHQNCPYSKLSRGNIDVEINLI"
     gene            6122..6394
                     /locus_tag="VKQ27_16865"
     CDS             6122..6394
                     /locus_tag="VKQ27_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HLY90655.1"
                     /translation="MKKSQATLAAALAISFVTLSTGAHAADQKKPMEKCYGVSLAGKN
                     DCAAGAGTSCAATSKVDYDGKAWTLVDKGTCTTIKTPKGYGSLVAK"
     gene            6394..6894
                     /locus_tag="VKQ27_16870"
     CDS             6394..6894
                     /locus_tag="VKQ27_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLY90656.1"
                     /translation="MSVSVDTADSARSADRGSVGGGLRLSRLIPDNLLLFMARIGIAP
                     VFFLSGRTKVEGILTIKDSTYDLFQSEYALPLISPNLAAHLATYSEHFFPILLVFGLL
                     TRPAAAALLGMTLVIEVFVYPDAWPTHLSWAALFLPLIVRGAGAWSLDHLLARAWRKP
                     GASTRR"
     gene            6941..7711
                     /locus_tag="VKQ27_16875"
     CDS             6941..7711
                     /locus_tag="VKQ27_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY90657.1"
                     /translation="MKLLALMAASTMALAGSAFAAPPAPASGAPSGIKNIVIVPDAFV
                     DGSGWRVAHDILIHKGYHVQVVQEHFASLDDDVSIANEIIRQQDGPTLLVGHGYGGAV
                     ISIAGAREKVRGLVYVGAVQPDVGEAVSQILASVPQPSNDIRTGRDGHLFFDPAKFGA
                     DYAGDLSTNRTDFMAASQAPATAAAFGGMAYVAAWRTKPSYAVVATEDRVLSPDLQRS
                     MYKRAGSTVTEIKASHAVYISQPEAVAGVIEQAATHAQ"
     gene            7803..8081
                     /locus_tag="VKQ27_16880"
     CDS             7803..8081
                     /locus_tag="VKQ27_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90658.1"
                     /translation="MSNKIKSGVSLATAAALMALSSVSIGSAAHAADGAQKEQPGRCY
                     GVNTCKGQSLCATAKHDCKGLNDCKGQGVVVKTKTDCLAAGGTLTEPK"
     gene            8147..8986
                     /locus_tag="VKQ27_16885"
     CDS             8147..8986
                     /locus_tag="VKQ27_16885"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HLY90659.1"
                     /translation="MSTSNPRFSGYGLGLRREHYRDFLETQVPVDFVEVISENFMVEG
                     GQPRHILRQVRERHPVALHGVSMSIGSADGLDRDYLRRLKALADEVDPLFVSDHLSWS
                     RIDGFNSHDLLPIPYTEEALDRVCANIAMAQDVLGRPMLFENPSSYLLFDGAMSEWEF
                     LAEMARRTGCDLLLDVNNIFVSATNHGFDPVAFLDGIPADRVRQIHLAGHSQGRDMLI
                     DTHDSPVCDDVWTLYAEALAQLGPVATMIERDDDIPPLVDLLDELARARVIGAAREQR
                     VAA"
     gene            8983..9729
                     /locus_tag="VKQ27_16890"
     CDS             8983..9729
                     /locus_tag="VKQ27_16890"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HLY90660.1"
                     /translation="MTLADLQQNFRSWLITGTDEVATRFGAGTSAGLSVYQNNYRTQL
                     MGCLEQSFPQVRGWMGEEAFRAAAISHIDDHPPHSWTLDVYAQTFPATLAALYPDNPD
                     LHELVWIEQALSDAFVGPDAEPLSADALAAIDWDTARLTVAPTLASRPATTNAEQIWT
                     ALQEGKPVPEGAMLAEPGGLIVWRRAFGAYLRQVDGIEYEALIELGRNGSFAALCTLL
                     VDRLGEEPGVARAADLLAQWLTHGLIVGNG"
     gene            9946..10266
                     /locus_tag="VKQ27_16895"
     CDS             9946..10266
                     /locus_tag="VKQ27_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90661.1"
                     /translation="MPVVDGVISSLDPHERSAVVVALNDAGRGHRTAPTAKGFRRTCY
                     KLYLRLTGHVAPQPLANPRLEAIRRFAEVTRRAGRVAETHVSAMRKQGFSRAQIDAIT
                     ALSA"
     gene            10282..10485
                     /locus_tag="VKQ27_16900"
     CDS             10282..10485
                     /locus_tag="VKQ27_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90662.1"
                     /translation="MAPASSEVIRIGVGVAVLSLLGLVAVVTARTLDSALLGIVVTGL
                     LIVTGAALARAIRSHPGPAHQRR"
     gene            complement(10668..11348)
                     /locus_tag="VKQ27_16905"
     CDS             complement(10668..11348)
                     /locus_tag="VKQ27_16905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY90663.1"
                     /translation="MRHLRTKLYASGAGLERYPLRSHRIGFEISVLYGRLPPVIKHLR
                     AINTHLDIDLRALSAPEQVAAIRNGAIDVGLGRELISDDHVEQTVIRQEPFLVALYAS
                     HILAADNPPSLRFADLAGDTFISYHDQSTGRRSDPVARLMDRANFVPVRKTEIRDVLA
                     DLGLVAAEAGICIVPATVQRMRSTDVCYKPLDEEDATSSIVLSVARGSTSPIVGQIKD
                     ELERLLQG"
     gene            12132..>12339
                     /locus_tag="VKQ27_16910"
     CDS             12132..>12339
                     /locus_tag="VKQ27_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90664.1"
                     /translation="MNVRFPVGGVTMAQNGLNIAIAPIMLAAVIGMLAWLLYYRSRSI
                     RDADQLISWFSALRQGDFSVSRVPS"
BASE COUNT         2130 a         3939 c         3941 g         2329 t
ORIGIN      
        1 gggttccgaa ttccatttca cgctgccccg cgcagagctg ggagacgccg atgagtcata
       61 ggcagatgat ccatatcatc gatgacgagg aggccgtgcg ccactcgctc gccatcatgc
      121 tccaggtggc gggctacgcg gttacggcgt ggccgggcgg tgcgagcttc ctgcgagagg
      181 ccgcgcacgt cgagcccggg tgcgtgctat tggatatcag gatgcccgag atggatggcc
      241 tggaagtcca gcgcacgatg caggagcacg gcatcgcctg gcccgtcgtc gtcctgacgg
      301 gccatggcga cgtcaccacg gcggtggcgg cgatgaaggc cggtgcgatc gacttcctgg
      361 aaaagccgct cgcgaagtcg acgttggtca ccgccatcga ggccgcgttg cagagtttcg
      421 aaggaacgga tgccgcgcgc cggcgggcgg ccctggcaag ccagcggatc gcacggctga
      481 cggcgcgcga gcaggaggtg ctgcgcggat tggctaacgg actgcccaac cggaacatcg
      541 cgcacgaact ggccatcagt ccgcgcaccg tcgagattca ccgcgcgaac ctgatggcca
      601 agcttggcgt ccggaccctg tccgacgcct tgcgcctggc attttcggct ggactcgatt
      661 cacccgtcaa ctgacgcgag atcgctcggc ggctggacgg cgccgtcagt cgttcctgtc
      721 accgagcggc agcatctcgt cctcgtcggc gtctagccgc cggtcgatct cctcggcgat
      781 gaggtcgagc tgttcgaagg tggccgccag ttcgaacact tccccgtcgc cggtttcgag
      841 atgcaggaga tagccatccc catggggcgt gatggtgaag cgcttgaggg acttggtcat
      901 cggggactcc tgcgatgctg gcctgggatt catgggggag gggtgggcaa ttccggctca
      961 gccctcgggg atgagctggc cgatcctcag aaaggtcagg ttatgcgtga gcgcgcgcac
     1021 ctcgccgtcg tcgccagctt cgagcgcgtt ggcgatcgag ctccagaccc cggcgctgtg
     1081 cgtgcagcgc tccctggtcg acgggtggat ggcggcgagc gcctgccggg ccgcccgcga
     1141 cgaccattcg cgatagaagc cgatgcgctg ggcttgcgtg atcaccagcc tccccgcgcg
     1201 acagcgggcg gatggcgtcg atcggcagcg ttgatggtca tcggaagcct cgtgtcttga
     1261 gcgagggcgc cgtccgtccc actgtcacga ggatgctgga cccgactccg tgatgccaag
     1321 tttgtagcac atggcccatc cggcgtcgcg tcggatccct atggcggaag cacggatcag
     1381 gggtgcgccg gaacgatccg gatcaggtgg agcgcgctcg gtaccgcgcg catctcggcg
     1441 atcgcggcgg cgcgaacttc gtccatcagc gcctcatcga aatcgtccgc ttcggggcgc
     1501 atctcgatgt cggtctccac gaccaggatc agccggcgct cgcggccgtc gatctcccat
     1561 tcgagccagc tgcgcaggac gttcggatgc gacgcgaggt cagccatggc cggctggccc
     1621 tatggcgcgc gcgccctcgc gccggacggc ataggccgta gcgagcttct catgcgcgat
     1681 ccgggcgcag cactcgccgg catcttgcgc ggcggcccgc gacgcctgtt cgcggtcggc
     1741 gagatagatg gcctcggtcg ctgtacacac aacgcatctc cctctggcaa gcgggagcat
     1801 gagcagggct ctcagtcgtc gggtgcctgc gaaagaaatc gaccggcgat ggctgcatcc
     1861 tatacctttg cgcgcggagc gaccatttta ttcccggcgg aatgatttgc ccctattgga
     1921 ggatggcgcg ccaggatcgg cctgatgcca tccgaggatt ccgaaatccc gcacgcccgc
     1981 tccatcggta tcgcacaggc ccatcgcacg ctttgcaacg ccacacgctt cctcggatcc
     2041 gggttgagcg acgccgggtc caccccgatc ggtggccgct atcgcgccaa ggtgatcggc
     2101 aatggcttgc gcgagctcga tcgcttcctg agcgtgctgc tcgacgaggt ggcgctggcg
     2161 gcgggctggg aacgcgccga tctgcagcgg ctcgccaatc tccgcaatac cgccaacaag
     2221 ctgggggtga tccacggctg gttggggcgt ccgacgcatg atctgccccg gctgcgcgcg
     2281 cttgggcgat gccgtgattg cctgtttcac tgcaacggcg tggtgcggcg cggcgacgag
     2341 cgccgggcag cgagcatgac cgtgggctgg cccgccgatg ctcgcggaga cgcggcgggc
     2401 gtacggctgg cgctgggcga gaacctcgcc gtcagtcccg ctgatctcgc ctggatctgc
     2461 gccttctatg accgtatcgc gggcgatctg gtagaagata tccgtggtgt tcgatccgat
     2521 ttcgcgagga ccgaacacga actgtcatgc tgaaggcagc agatgtttgc gatgctgtgc
     2581 aagatcagct cgccaagcat tatccttgct attgaatctg caagcgccca tatcgggcga
     2641 gctaacgtcg cctgcgacgg aatttgatgc tcgcgcgtcg ccttccctta cacgcttctg
     2701 gcttcgcggg tttccgcctg cgggacatcc cgttcggagg gactcttcat gccgatcggc
     2761 gccgtcctat cctgcatcgc caccgccttc caacgcggtt gagcgatcgg gctcgcgtcg
     2821 agatggcgcc ctatgatccc gaaggtgccg gggcgtccat cgcgggcgcc ggatggtgcc
     2881 gtcgggcacc gggcagggga cgtgccgtta gcgccccaca caccatcatg cgcgcatcgt
     2941 gcgcgccgag gatacatgaa ctttctaccg cctacccttc ctgtcgccgg cgcgaagccg
     3001 gacaagccct tctatccgca cgggtgtccc gcgcctcaat tccgccctcg atcggcgacc
     3061 ctgtcgcccg ccgaggtgcg gcggatcgtc gccgaactga tcggctgatc gccgcacctc
     3121 tctctctcaa ccggagcgca ccatgtcggt gacaagcgat ttctacctcg cccgtgcggc
     3181 cgaatgtgcc caggccgccg atgccgcggg gttggacaat gtccgcgatc gctttcgtcg
     3241 ttcggaagcc gcctggctgg cgatggccgg ccgcctccag cgcggcgagg cgttgcgcga
     3301 tgtcgccgcg gccgagaagg ccgcgcgggg cacgccatga agaagccgac attcgccgag
     3361 cgggtggcgc gctgcgccga cgtcttcacg acggtgaacg atcgccatcg cgaagatgcg
     3421 gctggtcgat ccccggccgg cgcacgtcga gcggcggcgc ctcccggcgg cggcgtggcg
     3481 cgtgcgcccc gacctgtttc ggggccgaga aaataaagcc gtggcaggga gatgctattt
     3541 ggtcgcgaag accccatatt agcgtcgctg acgtcgcctg cgacggatat cgggccgttt
     3601 cggcttccct ttgtccccac gagcttcacg aggccgggat gcgccacgct ggcgcatgcc
     3661 cgcaccatgg gcaaaggaat cccatatgat caccggaacc gtcaaattct tcaacgccga
     3721 caagggctat ggtttcattg ctccggaaga cggcagcggc gatgcgttcg tgcacatctc
     3781 ggccgtcgag cgcgccggca tgcgcacgct cgataaggat cagcgcatct cttatgagct
     3841 tgagaccgat cgccgtggca agacctcggc cgtcaatctc cagcaggcct gacgggggaa
     3901 ggggcgggcg ccggatcggc gcccgccccg catttttgcc gggcgcgcca acggccggga
     3961 ccatctcccg ggaagcgcgg gatcgatgac gcggaaggcc cgaaaggccg accggttcga
     4021 cgtgaaacag gccgcgcgcc gcctcaatgg gcgagcagca gcggcaggtc ggcggccgcg
     4081 agcatgtgac gagtggtgcc accgagcagc cagtcgacca tctcggaatg ccgataggcg
     4141 ccgcttacca gcagatcggc gcccaccgca tgcgcctccc cgatgatctg ggcaccgaga
     4201 tcggacccat gcttcggcac gacatgcagc gcaggctcga cgccggcctc gatcagcagg
     4261 cgcgagagcc cgagcgtggc atcgtcccga tcgccgatcc ggattgctga gacgcgcttg
     4321 gctgcacgca gccagggccc agcgccctcg attgcgtgtc gggcggcttc gccgttgctg
     4381 agtcccacca cgacatgatc gaagccggcc cgtgatccgc gccgccattc gcgcgggatg
     4441 atcagcaccg gcttgtcaca gcggaagatt gcggcatgga gtgcgtcgcc gctatccaga
     4501 ttggcttcgc gcgccatgac cagcatgacc acgtcggcgg ccccggcctc gcgacagacg
     4561 atttcctcct catggccggt cagcgtcttc cattgcacgg cgcagccgca cgccccggct
     4621 tcggtcttcc acgctgcgaa ggcgctccgc gtcgctttcg cgcgctgcca ggcggtgccc
     4681 tcctcgacca cgcggaactg ctgaatcgcg atctcctcgc tcgccgcgat catatggtcg
     4741 ggatcgacga tcacatggag cgcctccacc gaggcatggt cgagcgcgcg cgccgcgagc
     4801 gtcgccgcat cgaggcaggc gggcgcgctc ttcgcgccga cgagaatggc gagcagccgc
     4861 atggtgacct tcacccccgc cggatcataa catgcggcgc ccgaaatcgc gccatcccgt
     4921 cggcggggga ggcggatcag ttctgatccc gcgtatgctt gagcagattg tcccgcacgc
     4981 gaacgacgct gttctgcact aggccaaact cgtccgcggg aaggccggtc gccttgacca
     5041 gcgtcctctg caatcctctt tcgcgcagtt cccggcccgt ttcggtcagg gtgacgcgca
     5101 cctgccgctc gtcggcggga tcccgctggc gacggatata gcccatgccc tccagcttct
     5161 tgagcatggg cgtcagcgtg ttggattcga ggaagagctt ctcgccaagg ctgctgacgg
     5221 tctcgttgtc atgctcccac agcgccacga tcgtgatcca ctgggtgtag gtgacaccga
     5281 ggtcgtcgag cagcggcttg tacgcgcggc cataggcgag attggccgaa tagatcgcga
     5341 agcacaggaa atccgcgagt ttcggggcgt ctgggtcagt gggttcgata cgggccatgt
     5401 gtgtcctctt cagttcggta tctcgaatat agatcggatg cgatcggatc ggaaggccat
     5461 tgcagcaaat aaagcgcatg cgatgtaatc ggaagcggtt gacacccgcg tcgcgattcg
     5521 cctaataaga tcgtgtccga tcaaatcggg tccgatttat acgaggcaat gatgaccaag
     5581 atcgaaaacc tgctctacac tgccaagacg cacaccaccg gtggtcgcga cggtcacgca
     5641 cgcagctccg acggcctgct ggacgtccgg ctgtcgcagc cgggtggtcc gggcgccggc
     5701 accaatccgg aacaactttt tgccgccggc tggtcggcct gcttcatcgg cgcgatgggg
     5761 catgttgcgc gccaacagaa gctggccctg ccggccgatc tgtcggtgga tgccgaagtc
     5821 gaccttggca cggccgacgg tggctatctg ctgcaggccc ggctcaacgt cagcctgcct
     5881 ggtctcgacc gggaagtggc ccgcgcgctg gtcgacgccg cgcaccagaa ctgcccctat
     5941 tccaagcttt cccgcggtaa catcgacgtc gagatcaatc tgatctgacg ttggccgccg
     6001 gagcggggag gttcgtcccg gctctgcccc ctctctcccc agcgggggcg ggcttcctcg
     6061 ataacaaccg ggccggcgcc gtgcgggccc gaaccatgtc gtgcaccctc aggagatgat
     6121 catgaagaag tcacaggcca cgctggccgc cgccctcgcg atttcgttcg ttacgctgtc
     6181 caccggcgcc catgcggcgg atcagaagaa gcccatggag aaatgctacg gcgtctcgct
     6241 cgccggtaag aatgactgtg cggctggtgc cggaacgtcc tgcgccgcaa cctccaaggt
     6301 cgactatgac ggcaaggcat ggacgctcgt cgacaagggc acctgcacca cgatcaagac
     6361 gccgaagggc tatggctcgc tggtggccaa gtgatgagcg tgtccgtcga tacggctgac
     6421 agtgccaggt cggcggatcg gggatcggtg gggggaggac ttcggctctc ccgcctcatc
     6481 cccgacaatc tgttgctgtt catggcgcgg atcggcatcg cgcccgtctt cttcctgtcg
     6541 gggaggacga aggtcgaggg aatcctgacg atcaaggatt cgacctatga tctgtttcag
     6601 tccgaatatg ccctgccgct catcagcccg aatttggcgg cgcatctcgc cacctattcc
     6661 gagcatttct tcccgatcct gctcgtcttc gggttgctga cacgacccgc ggccgcggcg
     6721 ctgctcggca tgactttggt catcgaggtc ttcgtctatc ccgatgcctg gccgacccat
     6781 ctgagctggg ctgccctgtt cctgccgctg atcgtccgag gggcaggggc gtggtcgctc
     6841 gaccatcttc tcgcacgggc atggcggaaa cctggcgcga gcactcgccg ctgaggcggg
     6901 atcgcactca tccaccctat ccaacacagg tatcgcaagc atgaaattgt tagcactgat
     6961 ggccgccagt acgatggcac tggccggctc tgcattcgcc gcgcccccgg ctccggcttc
     7021 gggcgccccg tcgggcatca agaatatcgt catcgttccc gacgcctttg tcgacgggtc
     7081 gggttggcgc gtggcccacg atatcctgat ccacaagggc tatcatgtgc aggtggtgca
     7141 ggagcatttc gcctcgctcg acgatgatgt ctcgatcgcc aacgagatca tccggcagca
     7201 ggacgggccg accctgctcg tcggccacgg ctatggtggc gctgtgatct caatcgccgg
     7261 cgcccgggag aaggtgcgcg ggctggtcta tgtcggtgct gtgcagcccg acgtgggcga
     7321 agcggtgtcc cagatcctgg cgtcggtgcc gcagccgagc aacgacatcc gtaccggccg
     7381 cgacggtcat ctgttcttcg atcccgcgaa gttcggggcc gattatgcgg gcgacctgag
     7441 caccaaccgt accgatttca tggcggcatc gcaggcgccg gcgacggctg ctgccttcgg
     7501 cggcatggcg tatgtcgccg cctggcggac caagccgagc tatgcggtcg tggcgaccga
     7561 ggatcgtgtc ctcagccccg acctgcagcg ttcgatgtac aagcgcgccg gttcgacggt
     7621 gactgagatc aaggcgagcc acgccgtcta catctcgcag cccgaagccg tcgccggggt
     7681 gatcgagcag gcggccaccc acgcccagta attcggacga tcgtcgatcc gcgatctccc
     7741 cgaataagcg gcgactcgac cgtcgccatc cgattttccc ttttcctgat aggagccttc
     7801 ccatgtccaa taagatcaag tccggtgtca gcctcgccac cgccgccgcg ctgatggcgc
     7861 tgagctcggt gtcgatcgga agcgcggcgc acgccgccga tggtgcccag aaggagcagc
     7921 ccggccgctg ctatggcgtc aacacctgca agggccagag cctctgcgcg accgccaagc
     7981 atgactgcaa gggcctcaat gactgcaagg gccagggcgt ggtggtgaag accaagaccg
     8041 actgcctcgc ggccggcggc acgctcaccg agccgaagta atcgatcgac ggcccggtat
     8101 cctgacggtg ccgggccgtt cccttcctct caagcgagcg agcatgatgt ccacctccaa
     8161 cccccgcttt tccggctacg ggcttggctt gcgccgggag cattatcgcg atttcctgga
     8221 gacgcaggtt cccgtcgact ttgtcgaggt catttccgaa aacttcatgg tcgaaggcgg
     8281 gcaaccccgc catatcctgc gccaggtgcg cgagcgccat ccggtggcgc tgcacggcgt
     8341 atcgatgtcg atcggctcgg ccgacgggct cgaccgggac tatctgcggc gcttgaaggc
     8401 gctcgccgac gaggtggatc cgctctttgt gtccgatcat ctgagctggt cgcggatcga
     8461 cggcttcaac tcgcacgacc ttctgccaat tccctatacg gaggaggcgc tggaccgcgt
     8521 ctgcgccaat atcgcgatgg cgcaggacgt gcttgggcgg ccgatgctgt tcgagaatcc
     8581 ttccagctat cttctgttcg acggcgcgat gagcgagtgg gaattcctcg cggagatggc
     8641 ccggcggacg ggctgcgacc tgctgctcga cgtcaacaac atcttcgtca gcgcgaccaa
     8701 ccacggcttc gatccggtgg cgttcctgga cggcattccg gccgatcgtg ttcgccaaat
     8761 ccatctcgcc ggacacagtc agggccgtga tatgctgatc gacacgcatg acagtcctgt
     8821 ctgcgacgat gtctggacac tttatgccga ggcgctggcc cagctcggcc cggttgcgac
     8881 gatgatcgag cgcgacgatg acatcccgcc gctggtcgat ctgctcgacg aactggcgag
     8941 ggcgcgtgtc atcggggctg ctcgtgagca gcgggtggca gcatgacgct cgccgatctt
     9001 cagcaaaatt tccggtcgtg gctgatcacc ggcacggacg aagtcgccac ccgtttcggc
     9061 gccgggactt cggcggggct ttcggtctat cagaacaatt atcgcacaca gttgatgggc
     9121 tgtctggagc agtccttccc gcaagtgcgc gggtggatgg gcgaggaagc gttccgcgcc
     9181 gccgccatct cccatatcga cgatcatcct ccgcacagct ggacgcttga cgtctatgcg
     9241 cagaccttcc cggcgacgct ggcggcgctt tatccggaca acccggatct gcacgaactc
     9301 gtctggatcg aacaggcttt gtccgacgcg tttgtcgggc cggatgcgga accactgtct
     9361 gcggatgcgc tggcggcaat cgattgggac accgcgcggc tgaccgtcgc gcccacgctc
     9421 gccagccgcc cggcgacgac caatgccgag cagatctgga ccgccttgca ggagggtaaa
     9481 ccggtgcccg agggtgcgat gctcgccgaa ccaggcgggt tgatcgtctg gcggcgtgcg
     9541 ttcggcgcct acctccgaca ggtggacgga atcgaatatg aggccctcat cgagctgggc
     9601 cgcaacggaa gctttgcggc gctctgcacg ctgctggtcg atcgactggg cgaggagcct
     9661 ggtgtcgcaa gggccgcgga tctgctcgcc cagtggctga cccatggcct gatcgtgggg
     9721 aatggatagg cggcctcgcc gccgccatat atcggaaccg gccgatcgag gtttcacgcg
     9781 tcaggtcgaa cccgagctga tggtcgagcg cccgacgttc agcgcaccga tgtcgatcgt
     9841 ggccgtcgac atttgcatgc ttcgatccag caatttcgat cgaatcctac atgatcatgg
     9901 aagcccacac acgtacatgc ggatcattcc ccgtaggagg ttcgcatgcc tgttgtggat
     9961 ggtgtcatat catcccttga tccgcatgaa cggagcgcgg ttgtcgtcgc cttgaacgac
    10021 gccggtcgcg gccaccgcac ggctccgacg gccaagggct tccggcgcac atgctacaaa
    10081 ctttatctcc gcctcactgg ccatgttgca ccgcaaccgc tcgccaaccc tcggcttgag
    10141 gcgatccgtc gcttcgccga ggtgacgcgt cgcgcgggaa gggtcgccga gacgcatgtg
    10201 tcggcgatgc ggaaacaggg tttcagccga gcgcaaatcg acgcgatcac tgctctttca
    10261 gcctgaaatg gagagaagga catggctccc gcaagttcag aagtcattcg gattggcgtg
    10321 ggcgtcgcgg tgctgtcgct gctcgggctg gtcgcagtcg tgacggcgcg aacgctcgat
    10381 tccgcgttgc tcggcatcgt cgtcaccggc ctgctcatcg tcaccggtgc ggcgctcgcc
    10441 agagccatcc ggtcgcaccc cgggccggcg catcaacgtc gctaggccgg ccgcaagtcg
    10501 tgagcccgta cttccatctt atcggggaac agccaccagc ttccatgcag tgactcgatc
    10561 atgggcgact cgctcccgcg attatcccat gtgggcgccc cccgacggta tcgcggatgg
    10621 gatcgccgtc gcggagcaag cgtcgtttgc tgcgggttca tctcggatta tccctgcagc
    10681 aggcgctcca gctcgtcctt gatttgtccg acgattggcg atgtcgatcc gcgtgcgaca
    10741 ctcagtacga tggacgatgt cgcatcctcc tcgtccaaag gcttgtagca gacgtcggtg
    10801 cttcgcatcc gctgcacggt ggcgggcact atgcagattc cggcttccgc tgccacaaga
    10861 ccgagatctg ccaggacgtc ccggatttcg gtcttccgga ctggcacgaa attggcccgg
    10921 tccatgaggc gcgcgaccgg atccgagcgg cgccccgtgg actgatcatg ataggaaatg
    10981 aaggtatcgc ccgcgaggtc ggcaaagcgc agtgaaggcg ggttgtctgc cgccaaaatg
    11041 tggctggcgt acagcgcgac gagaaacggt tcctgtcgga tcacggtctg ttcgacatga
    11101 tcgtcgctga tcagttcgcg tcctaaccca acgtcgatcg ctccgtttcg aatggcggcg
    11161 acctgttccg gcgcggacag cgcgcgcagg tcgatgtcga gatgcgtgtt gatggcccgc
    11221 aaatgtttga tgacaggcgg caaacggcca tagagcacgg aaatctcgaa gccgatccga
    11281 tgcgatcgga gcggatatcg ctctagcccc gctcctgagg cgtaaagttt tgttcgcagg
    11341 tggcgcaccc ggcatgaaag caatgggtac tcggatcatt gggaaatttc tgtatccaac
    11401 ataatctagt ggcccttttc gcctcatgca cgaagccgct tcgcggcagg ggtgcttggg
    11461 gtgatgtagc gtggttttcc tgaggttttc gagcagggtg gagttctctt gagcgagagg
    11521 cgctccccat ggcacccccg tctaatgatc ggccgatcac accactgcgc cagcctgtgc
    11581 tggacgacat ggccgtgcac ggcccgcgcg agcacatacg gcgcgactgc attcgtcatt
    11641 ggcccgatcc ctgggaaatt gccctcgaca tggatgagaa gccgggggac cctcaatctg
    11701 gaaacgccgc tgaccaacgg ctgacggatg gcacgagggg ttccaacagc gccaccgatc
    11761 agatctcgtt cacatctttc aaaagattga accgcaataa actccgatat ttttcttgat
    11821 tttctactaa agtcgatatc gcgtgcgcga taaatttcac gataatttaa gtcgtacaac
    11881 ttaacgatga gcgtcaatta ttgacgatca atctaaacgc gcatttgcag gaaaacattt
    11941 cgatatcacc atgcacgtca ccgatcgcga agttcatgcc agttgaatgc agcaccttct
    12001 aacggcagaa tataaataga taattatatg tgcgtgaaat tttgtaaaat ttacaaaatt
    12061 tcacgtggga tagttaatgg catcacacgg caacatcgaa ttctcttgtg tcggcgcctg
    12121 cacgtttcct gatgaacgtc cggtttccgg ttggcggggt gactatggcg caaaacggcc
    12181 tgaacatcgc gatcgctcca atcatgctcg cagctgtcat cgggatgctg gcgtggctgc
    12241 tatattatcg atccagatcg atccgcgatg cagaccagct gatttcatgg tttagcgcgc
    12301 tgcgccaagg cgatttctcg gtttcaaggg tcccgtccg
//
LOCUS       DATGEM010000549         4267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_946926, whole genome shotgun sequence.
ACCESSION   DATGEM010000549 DATGEM010000000
VERSION     DATGEM010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946926"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /gene="metK"
                     /locus_tag="VKQ27_16915"
     CDS             <1..958
                     /gene="metK"
                     /locus_tag="VKQ27_16915"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLY90665.1"
                     /translation="AIHDIGYDQDGFSWKNAEVSVHLHAQSSDIAQGVDAGGNKDEGA
                     GDQGMMFGYACRETPGLMPAPIFYAHEILRRIRELRHAHDKSVAGLLPDAKSQVTLRY
                     IDGKPVGATAVVVSTQHVEDLEQAEIKRMLWPLVESTLPAGWMPPEREFYVNPTGKFV
                     IGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYACRYLAKNVVAAD
                     LADRCTIQIAYAIGVSQPLSVYVDLQGTGKGVDETRIEKALRDLVNLSPRGIREHLHL
                     NRPIYVPTSAYGHFGREPDPEKGTFTWEKTDLVPGLKAALGR"
     gene            907..1629
                     /locus_tag="VKQ27_16920"
     CDS             907..1629
                     /locus_tag="VKQ27_16920"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HLY90666.1"
                     /translation="MGEDRPGAGPEGRARALKPPPERLYGRARGHRLRPRQERLLKVT
                     LPRLLYDASWRPATLWLEIGFGGGEHALAQIAAHSDIGLIASEVFVNGICSLLSRLVP
                     EGGEASAPLPNNLRLWPDDARVLLRALPDDSLDRLFLLFPDPWPKARHAKRRFVHPAM
                     LPLLARVLKSGAEWRLSTDDPTYQAWVHEVMAAQTLFNAPPPVTERPPDWPPTRYEAK
                     ALREGRTPLYWTFTAPKAIPRP"
     gene            complement(1587..2759)
                     /locus_tag="VKQ27_16925"
     CDS             complement(1587..2759)
                     /locus_tag="VKQ27_16925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HLY90667.1"
                     /translation="MRRMVCLDGLRGALASYVMLSHTIPFAPLPYWLLWLFQHGGAGV
                     DMFFILSGLVIVQSLDSFKYRARPFLIARVARIYPVFLVVFALAVVVQPIATGFERMA
                     WIGPDSPARFIWSGGWPSAWGVFVVTHLTMTHGLFPNGVLPDVWVGFLGAAWSLSTEW
                     QFYVLALLLGKRLGLLRMAWLFVIMAGVALVWQAMVPEAWQFSRAFLPNKAQYFALGV
                     VSGGGMRRGWSAYWPVLVVVMALCGVQGGFDKLLPPLAWTICLAAQYFSFPVPVEAET
                     ISLSCEFPHLTRLLRPLWGRRGVLGWVAVVLASRPMVWLGAVSYCIYLVNEPVQKLLG
                     VTLSLLVRGDAAWFTVLWIPGAVLLPIVVASWLHKWIELPAQRYGRGIALGAVKVQ"
     gene            complement(3284..>4267)
                     /locus_tag="VKQ27_16930"
     CDS             complement(3284..>4267)
                     /locus_tag="VKQ27_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY90668.1"
                     /translation="PWGSAVSVIEIALWDLAGKAAGVPVYKLLGGKVRDHVRTYNGSL
                     RVPLGGYAPEHFAADCRRMMGLKEGFTLIKQPIAFHSPMRREVPGFYLGEPVETPFHG
                     WHDSGLLTGKGMQHLVDCVAAMKEVTGDAVGLALDCGPGWTLPDAIRFARAVEPFNLV
                     WLEDMLTGDYSPFVNADVYRELTQATSTPIHTGEQIYLRQNFKELIERNAVRVIGPDP
                     CDVGGIAELKWIAEFADLHGVLMAPHGTGDGLLGLAALVQVCATLPNNFIAFEYPSGN
                     PAWWYDIVDGLPDPIVRNGLIEVWDRPGLGVEINPEKARSHLAQEDAQFFE"
BASE COUNT          865 a         1534 c         1198 g          670 t
ORIGIN      
        1 ggcgatccac gatatcggct acgaccagga cggcttttcc tggaagaacg ccgaggtcag
       61 cgttcacctc cacgcgcagt catcggacat cgcgcaaggc gtggacgccg gcgggaacaa
      121 ggatgagggc gccggcgacc agggcatgat gttcggctac gcctgccgcg agacgcccgg
      181 cctgatgccg gccccgatct tctacgccca cgagatcctg cgccgcatcc gcgagctgcg
      241 ccacgcgcac gacaagtcgg tcgccggcct gctgcccgac gccaagagcc aggtcacgct
      301 acgctacatc gacggcaagc ccgtgggcgc gaccgccgtc gtggtttcca cccagcacgt
      361 cgaggacctg gagcaggccg agatcaagcg aatgctctgg ccgctcgtag agagcacgct
      421 gcccgccggc tggatgccgc cggaacgcga gttctacgtg aaccccaccg gcaagttcgt
      481 catcggcggc cccgacggcg actgcggtct gaccgggcgc aagatcatcg ttgataccta
      541 tggtggcgct gccccgcatg gcggcggtgc attcagcggc aaggacccca cgaaggtcga
      601 tcgctcggcc gcctatgcct gccgctatct cgcgaagaac gtggtcgcgg ccgatctggc
      661 cgatcgctgc accatccaga tcgcctacgc cattggcgtc tcccagccgt tgtcggtcta
      721 cgtggacctt cagggcaccg gtaagggcgt ggacgagacc aggatcgaga aagccttgcg
      781 cgacctggtt aacctctcgc cgcgcggtat ccgcgagcac ctgcatctga accgcccgat
      841 ttacgttccc acctcggcat atggacattt cggccgcgaa cctgacccgg agaagggcac
      901 cttcacgtgg gagaagaccg acctggtgcc gggcctgaag gccgcgctcg ggcgctgaag
      961 ccaccgcctg agcgcctcta tggccgcgcc cgcggccacc gcctccgccc tcggcaggaa
     1021 cgcctgctga aagtcaccct gccgcggctg ctctacgacg catcgtggcg gccggcgaca
     1081 ctctggctgg agatcggctt cggcggtggt gagcacgcgc tggcgcagat cgccgcacac
     1141 tccgatatcg ggttgatcgc cagcgaggta ttcgtcaacg gcatctgttc gctgctctct
     1201 cgcctggtgc cagagggcgg tgaggcctca gcgccgctgc ccaacaacct gcgcctatgg
     1261 cccgacgacg cccgtgtgct gctgcgcgca ctccccgacg attcgcttga ccgactgttc
     1321 ctgctgttcc ccgacccgtg gcccaaggcg cgccacgcca agcggcgctt cgtccacccg
     1381 gcgatgttgc ccctgctggc acgcgtcctg aaatcaggcg ccgaatggcg cctctcgacc
     1441 gatgatccga cataccaggc ctgggtccac gaggtcatgg ccgcgcaaac gctgttcaat
     1501 gcaccgcccc cggtcacgga acgcccgcca gactggccgc ccacgcgcta cgaagcgaag
     1561 gccctgcgcg agggccgcac tccgctctac tggaccttca ccgcgcccaa ggcgatacct
     1621 cgcccatagc gttgcgccgg caattcgatc catttgtgca gccaggacgc cacgacaatc
     1681 ggcagcagca ccgcgccggg tatccacagc acggtgaacc acgccgcgtc gccgcgcacc
     1741 agaagcgaca gcgtcacgcc gagcagcttc tgcaccggct cgttcaccaa atatatgcag
     1801 tacgacaccg ccccgagcca gaccatcggc cgcgatgcca acacaactgc cacccatccc
     1861 aacactcctc tccgccccca caaggggcgg agaagtcggg tgaggtgggg gaactcacaa
     1921 cttaagctta tcgtctccgc ctccactggg accgggaagg agaagtattg cgcggccagg
     1981 catatcgtcc acgccaatgg cggcagcagc ttatcaaagc ccccctgcac cccgcacagc
     2041 gccatcacca ccacaagcac cggccagtac gcgctccatc cccgccgcat cccccccccg
     2101 ctgacgaccc cgagcgcaaa atattgcgcc ttgttcggta ggaacgcgcg gctgaattgc
     2161 catgcctcgg gcaccatggc ctgccagacc agcgccacac ccgccatgat cacgaacagc
     2221 caggccatcc gcagcaatcc caaccgcttc cccagcagca gcgccagtac atagaactgc
     2281 cattccgtgg acaggctcca ggccgcccca aggaaaccaa cccacacatc aggcagcaca
     2341 ccattcggga acagtccgtg ggtcatcgtg agatgcgtca ccacgaacac gccccacgca
     2401 gatggccacc cgcccgacca gatgaaccgc gccgggctgt ccggcccgat ccacgccata
     2461 cgctcgaatc cggtggcaat tggctgcacc accacagcta atgcgaacac cacgaggaac
     2521 accggataga tccgcgcgac ccgcgcaatc aggaatggcc gtgcgcgata cttgaagcta
     2581 tcgagcgact gaacgatcac cagcccgctc aggatgaaga acatatcgac cccagccccg
     2641 ccatgctgga acagccagag cagccagtac ggcaacggtg cgaacggtat tgtgtggctg
     2701 agcatcacgt aactcgccag agccccacgc agcccatcca ggcacaccat ccgccgcatg
     2761 cacgcaacat gcgctgcatc gcttaaaata ccttgagcgc ccccacatcc gacccgcaca
     2821 ccatcctgtt tgccgcgatg ggctgccggc acggtcgccc cggtgttcgc cggcgctgac
     2881 aaattcatcc aatggcaatt aatcaatgca ataaatcccc tctccacccc cggagcagcg
     2941 aggatgggtg aggtggggac accgccatga ccgtgcagct aggaaccgtg atagcgcgca
     3001 ggctgcgccg cgggtccacg ttggaaatgc cgtcgagagc acccgatcgg ccgctatatc
     3061 atcgatttcg cctgcccgga atgtaagcta gcgatcgagt cggatggcag ccagcatgcc
     3121 atatgacagg gagcttacgc tcaacgttcg gctcaacttg ctacgtacgg ttatcgcatc
     3181 atcgggtttt ggaacaacga agtgtaggag aacattgaag gcgtggtgaa ggtcataccc
     3241 atgacggtcg aaacaccccc ttggggaggg agagggacag cgttcactcg aagaactgtg
     3301 catcttcctg cgccaaatga gaccgtgcct tctccggatt gatctccacc cccaacccgg
     3361 gccggtccca cacttcgatc agcccgttcc gcacgatcgg atcaggcagg ccatcgacaa
     3421 tatcgtacca ccatgccgga ttcccgctcg gatactcgaa cgcaatgaag ttgttcggca
     3481 gcgtcgcgca aacctgcaca agcgccgcca accccagtag tccatcgccc gtaccatgcg
     3541 gagccatcag cacaccatgc agatccgcga actccgcgat ccacttcagc tcggcaatgc
     3601 cgccaacatc gcatggatca ggcccgatca cccgcaccgc attgcgctcg atcagttcct
     3661 tgaagttctg ccgtagatag atctgttcgc ccgtgtggat gggcgtcgag gtcgcctgag
     3721 tcagctcgcg atagacatcc gcattgacga acgggctgta gtcgccggtc agcatatcct
     3781 cgagccagac caggttgaac ggttccaccg cgcgagcgaa ccggattgca tccggcaacg
     3841 tccaaccggg cccgcaatcc agcgccaatc ccaccgcatc gccagtgacc tccttcatcg
     3901 cggcaacgca atcgaccaga tgctgcatcc ccttcccggt cagcagcccg ctatcgtgcc
     3961 agccatggaa cggcgtctcg actggctcgc cgagataaaa tcccggtacc tcgcgccgca
     4021 tcggactatg gaacgcgatc ggctgtttta tgagggtaaa tccctccttc agtcccatca
     4081 tccgccggca atccgccgcg aaatgctccg gcgcatagcc tcccagcggg acgcgcagcg
     4141 atccattgta cgtgcgcacg tggtcacgca ccttgccgcc gagcaacttg tagaccggca
     4201 cgcccgccgc cttgccggcc aggtcccaca acgcgatctc gatcacgctg accgccgaac
     4261 cccacgg
//
LOCUS       DATGEM010000550         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_602299, whole genome shotgun sequence.
ACCESSION   DATGEM010000550 DATGEM010000000
VERSION     DATGEM010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602299"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..647
                     /locus_tag="VKQ27_16935"
     CDS             171..647
                     /locus_tag="VKQ27_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90669.1"
                     /translation="MIARGRQGAASVEFAIIGLAFISLLMFALDAGWQMVIDAALGVG
                     GRAAARFGTTGATVAPGITPAPTNRNDSIIGLVILNSGNLLQADRLQVATTSFANFAA
                     IGSGGAAGPGNAGQVVQYTFTYTQPYLTPIAPTVTGASQVVHSLQLVVQNEPFPQN"
     gene            625..1197
                     /locus_tag="VKQ27_16940"
     CDS             625..1197
                     /locus_tag="VKQ27_16940"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HLY90670.1"
                     /translation="MSRSPRIEAWRRGVATVEFALVLPFILLLMLAGADVTVYLRTAM
                     RIDETATELAVVVTQYSSLYQSDFSTLFSAAQTIAGTTPVTGLLGATIISGIVNSNGT
                     QTIAWQQRSPSARFTSQFGSLGSVPSLPNNYTLPTGGVLIAVEVATSASPWVFSARLM
                     PTSSISSIRTYALYQPRLGSLATVNAGSRP"
     gene            1233..2747
                     /locus_tag="VKQ27_16945"
     CDS             1233..2747
                     /locus_tag="VKQ27_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HLY90671.1"
                     /translation="MMTGLLAIPLVAMIGLAIDLTRVWLVRSRLQMSLDAAVLVVARN
                     LATGGTSTDGINLFWANWGRTSTTSTAGFMAATATTPVVHNPAPGGLSGSVQLTSTST
                     ISPTLLGVLNIGMVTVSAASTAQSAAYGLELALVLDNTGSMAGSSISSLITSSNQLLG
                     ILYGTADTQPNLWVSVVPFAATLNVGKTHTSWLVGGAINQAPYSPSTWMGCVMARTSK
                     TGAADGDDFNDRTPSQAPFQPFLYPSTYHMYPSSGPIVYYTGTGKNKTAHNYWYPGDN
                     DWTVSPNNIVEPDSSNNAVGPNLDCPSLAILPETASKAAVATVLNKMVPVYRGGTIIS
                     LGLQAGWWTISPNWQGLWGGPTMPLAYNTPYMKKAIVLMTDGNNEWYDWADGVPGQTP
                     PTAPPTGVPAWTADGDADFTAYGRLLTNTRSVSPSNITTTLNTWMSQMCTTIKSNGIT
                     IYTILFNNSNSATQTLFRNCASAPTDYFLSPTTATLQTAFQQIGRDLSTLRLSQ"
     gene            2816..4489
                     /locus_tag="VKQ27_16950"
     CDS             2816..4489
                     /locus_tag="VKQ27_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HLY90672.1"
                     /translation="MGKGLWTPRGPGLLSRREALALGMAGAALAAGGRRAMAQAAAKP
                     PSKPTGQVIIGLSQEPTVFNPLMPHIEVDQGVHWNVFTPLWNVNAQGQFVPQLATEVP
                     TIGNGGISEDGLEWRVKLRSGVVWHDGTPFSADDVKFTLELINNPHFRAYTRNGHEQV
                     TDIKVVSPTEITWRMKKSYAPYASILSWTFIVPQHILGKADDPNTAPFNNAPVGTGPF
                     KWSERMPGDHITLVANEHFFGTGPYLERVVFHYVPDLTALFTQFQTGAVDYTGIQGIT
                     ADHYNEAVKLPGRNVTAGPSGTCESITMNLTHPALRDLAVRQALYYAMDKKSIIDAIY
                     YGLPKPTETFLPSQSWAYNPDLPKQVYDVAKAKQILDAAGWKPGAGGIREKGGVKLSF
                     INSTTAGNHVREQAQELLQQSWKEIGADMQIKNMPAAVIWGDYYNMSKYDSVMVGQNE
                     MTGPDPDVTIYYSSKATMAKGGSGQNTMEYDNPKVDELLAQGATTLEQSKRIPIYREL
                     QSVIRQDLPMLPIFQYARIEGTKKGLMGYEPSVYVQANSWNVGQWYWAT"
     gene            4566..>5108
                     /locus_tag="VKQ27_16955"
     CDS             4566..>5108
                     /locus_tag="VKQ27_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90673.1"
                     /translation="MSLYLLRRLGQSLILLVLVSLIGFALIHLAPGGPLAQFTLTPGM
                     SSADLARIAHQMGLDRPLPVQYWEWFRRLLIGDWGLSYRDNQPVLAVIGSHVPATLEL
                     MISSTLIAVLIGTWVGVLGALRRYSIFDYLATIGAMIALSLPTFWFGLVVIYAFSVKL
                     GWLPAGGMYTVGDGSLLDYAH"
BASE COUNT          957 a         1442 c         1744 g          965 t
ORIGIN      
        1 ccattggcga gcgatccggg ggcagacagc aaagtgcgcg agaacctggc ggtcgccctg
       61 gctttggcag gcgaacgtga ggcggccggc aaggtgcttg cggcggacat gcccgatgac
      121 accgcctcgg tcgcgctggc gggttatgcg gcgctgaaga tgccagccaa atgatcgcgc
      181 gtggccggca aggtgcggcg tcggtcgagt tcgccatcat cgggctcgcg ttcatatcgc
      241 tgctgatgtt cgcgctggat gccggttggc agatggtgat cgatgcggcg ctgggtgttg
      301 gtgggagagc ggccgcgcgg ttcggcacga ccggtgcgac agttgccccc ggcatcacgc
      361 cggcgccaac aaaccgcaac gactcgatca tcggtctcgt gatcctcaat tcgggcaatc
      421 tgctccaggc ggaccgcctg caggttgcca cgaccagctt tgccaatttc gcggccatcg
      481 gcagcggcgg agcagcgggg ccgggcaatg ccggacaggt ggtgcaatat acattcacgt
      541 atacgcaacc ttacctgacg ccgattgcgc cgacggttac cggcgcgtca caggttgtgc
      601 acagtttgca actggtcgtg cagaatgagc cgttccccca gaattgaagc ttggcggcgc
      661 ggcgttgcca cggtcgaatt cgcgctggtg ttgccgttca tcctgctgct gatgctcgcg
      721 ggcgccgatg tgaccgtgta tttgcggaca gccatgcgga tcgatgagac ggcgaccgag
      781 ctcgccgttg tggtaaccca gtattcgagc ttgtaccaaa gcgatttttc gacattgttc
      841 agcgccgcac agacgattgc gggaaccaca ccagtaaccg ggctgctggg tgcgacgatc
      901 atttcgggaa tcgtgaacag caatggcacg cagacaattg cctggcagca gcgcagtccg
      961 tcggcgcggt tcaccagcca gttcggtagt ttgggcagcg tgccgagttt gcctaacaac
     1021 tacacattgc cgacgggagg cgtgctgatc gcggtggagg tggcgacgtc ggcgtcgccc
     1081 tgggtgttca gtgcgcggct gatgccgaca tccagcatca gttcgatccg gacgtatgcg
     1141 ctgtatcagc ctcgtctggg ttcgctggcc acggtgaatg caggaagccg gccatgatgc
     1201 gatggtgccg acggaagcgg ggatcggtca cgatgatgac cgggctgctg gcgattccgc
     1261 tggttgcgat gatcggcctg gcaatcgatc tgacgcgcgt gtggctggtg cgatcgcggc
     1321 tgcaaatgtc tctggatgcg gctgtgctgg tggtggcgcg caatctggcc acgggcggca
     1381 ccagcacgga cgggatcaac ctgttctggg cgaactgggg ccggacatcg actacaagca
     1441 cggcgggttt catggcggcc accgcgacta cccctgtggt gcacaatccg gcgccgggcg
     1501 gactctcggg cagtgtgcag ctgaccagca cgtcgactat ttcgccgacg ttgctcggcg
     1561 tgctgaatat cgggatggtg acggtgagcg ccgcatcgac ggcgcagagt gccgcctacg
     1621 ggttggaact cgcgctggtg ctggacaaca cagggtcgat ggcggggtcg tcgatctcgt
     1681 cgctgatcac ttcatccaat caactgctgg gcattctgta tggtactgcg gacacgcagc
     1741 cgaatctgtg ggtgtcggtg gttccgttcg cggcgacgct caacgtcggc aagacacata
     1801 cgagttggct ggtaggcggc gcaatcaatc aggcacccta ttcgccctcg acgtggatgg
     1861 gctgtgtgat ggcgcgaacg tcgaagacag gggctgcgga cggcgacgat ttcaatgaca
     1921 ggacgccgtc gcaggcgcca tttcagccat tcctgtatcc ttcaacgtat cacatgtatc
     1981 cgtcttcggg accgatcgtg tattacacgg gaaccgggaa gaacaagacg gcgcacaact
     2041 actggtatcc gggtgacaac gattggacag tgtcgccgaa caatatcgtt gagcccgatt
     2101 cgagcaacaa cgcggtcgga cccaaccttg attgtccatc gctggcgatc ctgccggaga
     2161 cggcgagcaa ggctgcggtc gccacggtgt tgaacaagat ggttccggtg tatcgcggcg
     2221 gcacgatcat cagtcttggt ttgcaggcgg gttggtggac catcagcccg aactggcagg
     2281 ggctgtgggg cggcccgacg atgccgttgg cctacaacac gccctacatg aagaaggcca
     2341 tcgtgctgat gaccgatggc aacaacgaat ggtacgactg ggcagacggt gtgcctggac
     2401 agacgccacc gacggcgcca ccgactggcg tgccggcgtg gacggctgac ggtgacgccg
     2461 atttcaccgc ctacgggcgc ctgctgacca acacgcggag cgtttcgcca tcgaacatca
     2521 cgacgacgct gaatacgtgg atgtcgcaga tgtgcacgac catcaagtca aatgggatca
     2581 cgatctacac gatcctgttc aacaatagta acagtgcaac gcagacgcta ttccggaatt
     2641 gcgcgtctgc gccgaccgac tatttcctca gcccgaccac cgcgactttg cagactgcgt
     2701 tccagcagat cggacgtgac ctttcgacct tgcgtctgtc gcaatagcgc ttcgggcttg
     2761 gccctcggtg ggcggacgtt ctagtgtctg tccgagaaga aaagtggggg ctgacatggg
     2821 caagggactt tggactcccc gtggtccggg gctgctgtcg cgtcgtgagg cactggcgct
     2881 tggcatggcc ggcgcggctt tggccgcggg aggcaggcgt gcgatggcgc aagcggccgc
     2941 caaaccgccg tccaagccca ccggccaggt catcatcggg ctgtcgcagg agccgacagt
     3001 gttcaatccg ctgatgccgc atatcgaggt ggaccagggt gtccattgga acgtgttcac
     3061 cccgctgtgg aacgtgaatg cgcagggtca gttcgtgccg cagctcgcca ccgaggttcc
     3121 gacgatcggc aatggcggga tctcggagga cggcctggag tggcgggtga agctgcggtc
     3181 cggggtggtg tggcatgacg gaacgccgtt cagcgctgac gatgtgaagt tcacgctgga
     3241 gctgatcaac aatccgcact tccgtgcgta cacgcgcaac gggcatgagc aggtgaccga
     3301 catcaaggtg gtcagtccga cggagatcac ctggcggatg aagaagagct acgcgccgta
     3361 tgcgtccatc ctgtcgtgga cgttcatcgt gccgcagcac atcctgggca aggcggacga
     3421 tcccaacacg gcgccgttca acaacgcgcc ggtcggcacg gggccgttca agtggagcga
     3481 gcggatgccg ggcgaccaca tcacgctggt cgcgaacgag cacttcttcg gcaccgggcc
     3541 gtatctggag cgggtggtgt tccactacgt gccggatctg acggcgctgt tcacgcagtt
     3601 ccagaccggg gcagtggatt ataccggcat ccaggggatc accgcggatc actacaatga
     3661 ggcagtgaag ctccctggcc gcaatgtgac ggcagggcca tcggggacgt gcgaaagcat
     3721 cacgatgaac ctgacgcatc cggcgctgcg cgatctggcg gtgcggcagg cgctgtacta
     3781 cgcgatggac aagaagtcga tcatcgacgc gatctattat ggcttgccga agccgacgga
     3841 gacgttcctg ccgagccagt cctgggcgta caatcccgat ctgccgaagc aggtatacga
     3901 cgtggcgaag gcgaagcaga tcctcgatgc ggcgggatgg aagcccggtg cgggaggcat
     3961 tcgtgagaag ggtggcgtga agctgtcgtt tatcaactcg acgacggcgg gcaaccacgt
     4021 gcgcgagcag gcgcaggagt tgctgcagca atcgtggaaa gagattggcg cggacatgca
     4081 gatcaagaac atgccggcgg cggtgatctg gggcgactac tacaacatgt ccaagtatga
     4141 ctcggtgatg gtcgggcaga acgagatgac aggccctgat ccggacgtga cgatctacta
     4201 cagcagcaag gcgacgatgg cgaagggcgg atccgggcag aacaccatgg agtacgacaa
     4261 ccccaaggta gacgagctgc tggcacaggg cgccacgacg ctggagcaga gcaagcgcat
     4321 cccgatctac cgggagctcc agtcggtgat ccggcaggac ctgccgatgc tgcccatctt
     4381 ccagtatgcg cggattgagg ggacgaagaa gggactgatg ggctacgagc ccagtgtcta
     4441 cgtgcaggcg aactcttgga atgttgggca gtggtattgg gcgacctaag cggcgcgttc
     4501 ccctacctca ccctaaccct ctccacccca tgggcggaga ggggaccctg atgtaggcgc
     4561 tcggtgtgag cctgtatctg ctgcggcggt tggggcagag cctgattctg ttggtgctgg
     4621 tgtcgttgat cgggttcgcg ttgatccatc tcgcaccagg aggaccactg gcgcagttca
     4681 cgctgacacc cgggatgtca tcggctgacc tggcgcgcat tgcgcatcag atggggctcg
     4741 atcggccgtt gccggtgcaa tactgggagt ggttccggcg gctgctgatc ggtgactggg
     4801 ggttgtcgta ccgggacaac cagccggtgc tggcggtgat cggctcgcat gtgccggcga
     4861 cgctggagct gatgatcagc tccacgctga tcgccgtgct gatcggcacg tgggtgggcg
     4921 tgctgggggc actgcggcgc tactcgatat tcgactatct ggcgacgatt ggcgcgatga
     4981 ttgctttgtc gctgccaaca ttctggttcg ggctggtggt gatctacgcg ttctcggtga
     5041 agctgggctg gttgccggca ggtggtatgt acaccgtcgg cgatgggtcg ctgctcgact
     5101 atgcgcac
//
LOCUS       DATGEM010000551         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_602554, whole genome shotgun sequence.
ACCESSION   DATGEM010000551 DATGEM010000000
VERSION     DATGEM010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602554"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VKQ27_16960"
     CDS             complement(<1..404)
                     /locus_tag="VKQ27_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HLY90674.1"
                     /translation="MTSSLPVNEMIDLKRGMISREIFVSSDFYSEELDKLFTRAWLFV
                     GHESLIPKPGDFFTSRMGEESVILCRDAAGAVHVFLNSCRHRGMKVCRYEQGNTSLFV
                     CPYHSWSYTTDGKLQGVPLYRALYEGTLNRDE"
     gene            complement(418..1344)
                     /locus_tag="VKQ27_16965"
     CDS             complement(418..1344)
                     /locus_tag="VKQ27_16965"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HLY90675.1"
                     /translation="MAALDPFSWSRSPWRQATLATSFSTSGIGLHSGETAYVTVHPAP
                     PDTGRIVRRDGQAIPALWTHRVQQYLCTGIRGDSGAIVRTVEHLLASLSVFGIDNVLI
                     EIEGDEVPIFDGSAVPWCHAILKSGVLEQDVPRLHLRMLEPVEVREPTGSWLRIEPAQ
                     EFSVSVTSDPPGFGMLTWDGEPTPWTFLCDIAPSRSHGPILSFPSKLFFGGTRQPKLR
                     GAGLATVGLTFRGRYINGMRVPDEPVRHRVLDLIGDLALAGAPLVGRVTGHRPRHDLN
                     NMLVAAIMQRPQAWERVPLEPLRPKGAHSAQP"
     gene            1594..2403
                     /locus_tag="VKQ27_16970"
     CDS             1594..2403
                     /locus_tag="VKQ27_16970"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF021205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HLY90676.1"
                     /translation="MALVRGFMLVPGELNGNRAMFVVDTGAEATMVTPQAATEFGLDR
                     DHFNRTIIHGIGGQITTFNARVTSFGIGNQELLDRGVAVGPLPNVPATNPPIAGLLGA
                     DYLSDYDLDLDVPRRTLTLYSMDGCGDNFVPPWHLARSIVPIRREHKDLLMLDVMVDG
                     ERIDAMFDSGARVTTLSASAAGRLGIDEVAMAHDRSITQSGVDRNAVSGHVHQFDQVQ
                     VGREVFHDPHLEVAALMLPNAEMLLGADYLRTRHIWVSYTAHKLFIAPPGQ"
     gene            complement(2416..2778)
                     /locus_tag="VKQ27_16975"
     CDS             complement(2416..2778)
                     /locus_tag="VKQ27_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY90677.1"
                     /translation="MFKDWQDTATQLSGDIRTLRSGAPDVMKGFSVLAQAALKSDALE
                     TKTKELIALAIAVATRCDGCIAFHAEAAMKQGATRAEVLETMGMAIYMGAGPSVMYAA
                     QALEAFDQFSNKATAHAA"
     gene            complement(2790..3515)
                     /locus_tag="VKQ27_16980"
     CDS             complement(2790..3515)
                     /locus_tag="VKQ27_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLY90678.1"
                     /translation="MALLSPLMAGIASLCSPGPLGGGLVVGLFLAGAAGSTMHCVPMC
                     GGFVLGQVADGMARIPATRLCEWRRITGGALLPYHLGRITTYAGLGALAGLGAGRLPW
                     FATISAALLLLGAVLFLLHVARVLPGLECAPVAWTRFVTLAARRARGGYPLGIALGFL
                     PCGFLYAALATAAASGSPFLGAGAMLAFGLGTAPALIAVGIAGQAAGRRWQRGIAVAA
                     PTVMLLNAALLIVLALRGFAFSL"
     gene            3619..3816
                     /locus_tag="VKQ27_16985"
     CDS             3619..3816
                     /locus_tag="VKQ27_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90679.1"
                     /translation="MDIEPSDFIVGLLMLVFGIVGLILASGAMDDEMYVFGLSLFGFA
                     TVFEFGLLRRHFDKAEARRHG"
     gene            3809..>4369
                     /locus_tag="VKQ27_16990"
     CDS             3809..>4369
                     /locus_tag="VKQ27_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLY90680.1"
                     /translation="MADVGIGVLRPAPEYNEAIVKKFLIASVFWGVVAFLVGVYIATE
                     LTWPSFNLGLSFINFGRLRPVHTSAAIFAFGGCALFATSFHVVQRTCRARLFGGEALA
                     NFVFWGYQFFIVMAALSYVMGFSQSQEYAEPEWHIDLWLTVVWVCYAIIFIGTLMKRE
                     EPHIYVANWFYLAFILTVAVLHIGNNI"
BASE COUNT          773 a         1316 c         1441 g          839 t
ORIGIN      
        1 cattcgtcgc ggttcagcgt gccttcgtag agcgcgcgat acagcggcac gccttggagt
       61 ttgccgtcgg tggtgtagct ccaggagtgg taggggcaga cgaacagcga cgtgttgccc
      121 tgctcgtagc gacagacctt catgccgcga tggcggcagg agttcaggaa cacatgcacg
      181 gcgccggctg cgtcgcgaca caggataacc gattcctcac ccatgcggga ggtgaagaaa
      241 tcacccggct tggggatcag gctttcgtgt ccgacgaaca gccatgcgcg ggtgaagagt
      301 ttatccagtt cctcgctgta gaaatcggac gagacgaaga tttcgcggct gatcatgccg
      361 cgcttcaggt cgatcatctc gttcacgggc agcgacgagg tcatccgccg aacctcccta
      421 tggttgggcg gagtgtgctc ccttgggtcg caggggttca agtgggacgc gttcccacgc
      481 ctgggggcgc tgcatgatgg cggcgaccag catgttgttg aggtcgtgac gggggcgatg
      541 gccggtgaca cggccgacca gcggcgcgcc ggccagtgcg aggtcgccga tcaggtctag
      601 cacccggtgc cgcaccggtt catccggcac gcgcatgccg ttgatgtagc ggccgcggaa
      661 cgtcaggccc acggtggcga ggccggcgcc gcgtaatttc ggctgcctcg ttccgccgaa
      721 gaacagtttc gatgggaagc tgaggatcgg gccatggctg cgcgatggcg cgatatcgca
      781 caggaaggtc cacggcgttg gctcgccgtc ccaggtgagc atgccgaagc cgggtggatc
      841 ggaggtgacg ctgaccgaga attcctgcgc cggttcgatg cgtagccagg agccggtggg
      901 ttcgcgcacc tcgaccggtt cgagcatgcg gagatggagg cgcggcacgt cctgttcgag
      961 cacgccagat ttcagaattg catggcacca cggcaccgcg ctgccgtcga atatcggcac
     1021 ttcgtcgcct tcgatctcga tcagcacatt gtcgatgccg aagacggaga ggctggccag
     1081 caggtgctcc acagtgcgga cgattgcgcc ggagtcgccg cggatgccag tgcacaggta
     1141 ttgctgcacg cgatgcgtcc agagcgcggg gatggcctgt ccgtcgcggc gcacgatgcg
     1201 gccggtgtcg ggcggcgccg ggtgcactgt cacatacgcc gtttcgccgc tgtgcaggcc
     1261 gataccggag gttgagaacg acgttgccag tgtcgcctgc cgccacggtg agcggctcca
     1321 actgaagggg tcaagtgctg ccaagtgccg cctcatatgt tgttgggagt atagcacagt
     1381 gggtggctca actggctaca tgggtgggag tttcacttga ccttacccaa tcggggtcgt
     1441 aacagaattg tcagcattac atgttggtgc cgtatgcgtt tgatgcagac tattgtattt
     1501 gttactttgt tgttactcgc ggcatgccag aatccatcgg gatcatcgac cactgcacat
     1561 cgctgcactg ttcagcgtac ggcagtcctg ccagtggcgc tcgtacgcgg cttcatgttg
     1621 gtgcctggag agctcaacgg caatcgggcg atgttcgtgg tggacaccgg tgcggaggcc
     1681 acgatggtga cgccgcaggc agccacggaa ttcggtctgg accgcgacca cttcaatcgc
     1741 acgataatcc atgggatagg cgggcagatc accacgttca atgcacgcgt aacatcgttc
     1801 gggatcggca atcaggaatt gctggatcgt ggcgtggctg ttggtccgtt gccgaatgtt
     1861 cctgctacca atccgccgat cgcgggtctc ctgggtgccg actacctgtc ggattacgac
     1921 ctggacctcg acgttccacg ccgcacgctg acgctctaca gcatggacgg ctgtggcgac
     1981 aattttgtgc cgccgtggca cctggcgcgg tccatcgtgc cgatccggcg cgagcacaag
     2041 gatctgctga tgctggatgt gatggtcgat ggagaaagga tcgacgcgat gttcgattcc
     2101 ggggcgcgtg tcacgacgct cagtgcgtcg gcggcagggc ggctcggcat cgatgaagtg
     2161 gcgatggcgc atgaccgctc gatcacgcag agcggcgtcg atcggaatgc cgtgtctgga
     2221 catgtccatc agttcgatca ggtccaggtc ggtcgggagg ttttccacga tccgcatctg
     2281 gaggtcgcgg ccctgatgct gccgaatgcc gagatgctgc tgggcgcaga ctatctgcga
     2341 acacgccaca tctgggtgtc ctatacggcg cacaagttgt tcattgcgcc tccggggcag
     2401 tagccaacca acgggctacg ccgcatgcgc cgtggctttg ttgctgaatt gatcgaatgc
     2461 ttccagcgcc tgcgccgcgt acatcacgct cgggccagcg cccatgtaga tcgccatgcc
     2521 catggtttcg aggacctcgg cgcgcgtggc accctgcttc atggcggctt ccgcatggaa
     2581 ggcgatgcaa ccgtcgcacc gcgtggcgac cgcgatcgcg agcgcgatca actccttggt
     2641 cttggtctca agcgcgtcag acttcaacgc cgcctgtgcc aggaccgaga agcccttcat
     2701 cacgtcgggg gcaccagagc gcaacgtccg gatatcaccg ctgagctgcg ttgccgtgtc
     2761 ctgccagtcc ttgaacatgt caccctccat cacagactga acgcaaatcc tcggagcgcc
     2821 agcacgatga gcagtgcggc gttcaacagc atgaccgtgg gtgcggcgac ggcgatgccg
     2881 cgctgccaac gacggccggc cgcctgtccg gcgatgccaa cggcgatcaa cgccggtgcg
     2941 gtgcccaggc cgaacgccag catggccccg gcgccaagga acggactgcc gctcgcggcg
     3001 gcggtggcga gcgcggcgta gaggaagccg catggcagga agcccaaagc gatccccagt
     3061 ggatagccgc ctcgagcgcg ccgtgccgcc aaagtaacga aacgtgtcca ggcaaccggg
     3121 gcgcattcca gtccggggag gacgcgggcc acgtgcagca ggaacagtac ggccccgagc
     3181 agcagcaacg cagcagagat tgtggcgaac cacggcagtc gtccggcacc caagcccgcc
     3241 agcgcaccca gaccggcata cgtggtgatc cggcccaggt ggtagggcag caacgcgccg
     3301 ccggtgatgc gccgccattc gcagagacgt gtcgcgggta tccgcgccat gccgtcggcg
     3361 acctggccga gcacgaatcc gccgcacatc ggcacgcagt gcatggtgct gcctgcggca
     3421 ccagccagga acaggccgac taccagcccg ccgccgagcg gtcccgggct gcagagcgac
     3481 gcaattccgg ccatcagcgg ggagagcagc gccatgcgtg catcgaacct ccatcaaacc
     3541 cccggataca ttgatccaga tcaaggttcc attaactcga gcagggcact gtccgtccgg
     3601 cacccaccgg agcgctccat ggacatcgag ccaagtgatt tcattgtcgg cctgctgatg
     3661 ctcgtgttcg gcatcgtcgg cttgatcctc gcctcggggg cgatggacga tgagatgtac
     3721 gtcttcggcc tgagcttgtt cggcttcgcc acggtgttcg agttcggtct gctccgccgg
     3781 cacttcgaca aggcagaggc ccggcgacat ggctgacgtc ggcatcggcg tgttgcgtcc
     3841 ggcgccggaa tacaatgagg cgatcgtcaa gaaatttctg attgcctcgg tgttctgggg
     3901 agttgtggcg ttcctggtcg gtgtttatat cgcgactgaa ctcacctggc cgtcgttcaa
     3961 tctgggtctg tcgttcatca acttcgggcg gctgcgtccg gtgcacacct cggcggcgat
     4021 cttcgcgttc ggtggatgcg cgctgttcgc cacgtcgttc catgtcgtgc agcggacttg
     4081 ccgcgcgcgg ctgttcggcg gcgaggcgtt ggccaacttc gtgttctggg gctaccagtt
     4141 cttcatcgtc atggcggcgc tgtcctatgt gatggggttc agccagagcc aggaatatgc
     4201 cgaacctgag tggcatatcg atctgtggct gaccgtcgtt tgggtctgct acgcgattat
     4261 cttcatcggc acgctgatga agcgggagga accgcacatc tacgtggcaa actggttcta
     4321 cctcgccttc atcctgacgg ttgccgtgct gcatatcggc aacaatatc
//
LOCUS       DATGEM010000552         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1189306, whole genome shotgun sequence.
ACCESSION   DATGEM010000552 DATGEM010000000
VERSION     DATGEM010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189306"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VKQ27_16995"
     CDS             complement(<1..501)
                     /locus_tag="VKQ27_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLY90681.1"
                     /translation="MSTLHMTTATIYEAPRGNARPGETIVTSTCGHNCGGRCVVNAHV
                     RDDRIVHISTDMRRWDPKHPPLPACARGVGQIERTYHPDRLQHPMRRTGPRGSGQFER
                     ITWDEALDEVARELLRVRASYGNAAILDASRTGSQSMLHGRVAAQRFLNMFGGCTDLW
                     SNMSAEA"
     gene            572..1747
                     /locus_tag="VKQ27_17000"
     CDS             572..1747
                     /locus_tag="VKQ27_17000"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY90682.1"
                     /translation="MNYVGPTASDPVARALAIGPEIEAGAVDIERTQRIHEPLLSQIH
                     EARLCRMLLPRSVDGDEVEPWVYLRAIEEVSRHDGSVGWNVFVANSSALIAAFLEPEP
                     AGAIFADPRAVVAWGPPNQSKATAVGGGYRVTGQWAFASGCRQATWMGAHCHVVEADG
                     SLRLNAHGKPTVRTLLVPVEQATLIDGSWDVIGMRGTMSGAYRLDDVFVPEAFSSTRE
                     DPTLRRESGRLYAYPMQGLYAVGVAGVALGIAGAMLDAFKELATQKAPRNQGRLADNT
                     VVQATVAQMQARLDASRAYLVDTLTAIWSADDSWVIDVPARARVRLGCTFAIQNAEAI
                     ADYAYKSAGVDAIFLGTAFERRFRDMHTLSQQIQSRAAHFEAVGQILLGIDPPGTFF"
     gene            complement(1712..2728)
                     /locus_tag="VKQ27_17005"
     CDS             complement(1712..2728)
                     /locus_tag="VKQ27_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLY90683.1"
                     /translation="MGEHRPDFAARLRQVMADINVSRVMLSRELGVDKSVIGRWLGGV
                     NQPTGHNLTRLTDIVRRYRPEVTLDFWERPALDPCAEPAVLQPAAAEGLRITGLRAER
                     QPRIDANYLGLWGGFYQSTQNRGSVVMSVMHIGNSADGLSCVFTEGKVSAAGIAVAIG
                     PRLHVILEIEPLHDRLCLFVFNGVLTPDAIGMDGTYMISAGDTSTCATASPIVLFRIG
                     DTSDYTRVGGLSGVMQALYPVNAQNIQDSILACDPTAGLSSLIPEQVLRAVCVQVGVA
                     RADGETDHVMRMPASRSLPQSHFGVRELPASAPLIMTRNAIRRVLGLEVACLEERSRR
                     VDAE"
BASE COUNT          497 a          876 c          928 g          496 t
ORIGIN      
        1 ggcctcggcc gacatattcg accacagatc ggtgcagccg ccgaacatgt tgaggaaccg
       61 ctgcgccgcg acacggccgt ggagcatcga ttgcgacccg gtgcgcgacg catccagaat
      121 cgctgcattg ccgtagctgg cacgaacccg cagcagttcg cgggcgactt cgtcgagggc
      181 ctcgtcccag gtgatgcgct cgaactgccc ggaaccgcgc gggccggtac gccgcatagg
      241 atgttgcagg cgatcgggat gataggtgcg ctcgatctgg ccgacaccac gcgcgcaggc
      301 cggcagcggc ggatgtttgg gatcccagcg ccgcatatcc gtgctgatat gcacgatgcg
      361 atcatcccgc acatgagcgt tgacaacgca acgcccgccg cagttgtggc cacaggtgct
      421 ggtcacaatg gtttcgcctg gccgcgcgtt gccgcgcggt gcttcataga tggtcgctgt
      481 cgtcatgtgc agagtggaca tggacggcgg tgcgttcgtc aaccggccag cccgagttga
      541 cagccagcac gcgacgcgcc gctaatgcgg catgaactat gttggcccta ctgcgtctga
      601 cccggttgcc cgagcgctgg ccatcggccc ggagatcgaa gccggcgccg tggatatcga
      661 acgaacgcag cgcatccacg agccattatt gtcgcaaatt cacgaagcac ggctctgccg
      721 gatgctgctg ccacgttcgg tggacggcga cgaggtggag ccctgggtgt atctacgcgc
      781 catcgaggag gtcagccgcc acgacggttc tgtcggctgg aatgtgttcg tcgccaacag
      841 ttcggcgctg atcgcagcgt ttctggagcc ggagccggcg ggggcgatct tcgccgaccc
      901 acgggcggtc gtcgcgtggg gaccgccgaa ccagtcgaaa gcgaccgcgg tgggcggggg
      961 ctaccgtgtc acgggtcagt gggcctttgc gtcgggctgc cgccaggcca cgtggatggg
     1021 ggcacattgc cacgtagtgg aggctgacgg ctcgctgcgt ctcaatgccc acgggaagcc
     1081 gacggtacgc acgctgctgg tgccggtcga gcaggccaca ttgatcgacg gctcgtggga
     1141 tgtcatcggc atgcgcggca cgatgtccgg cgcctatcgc ctcgacgacg tgttcgtgcc
     1201 ggaagccttc agtagcacgc gcgaggaccc aacgctgcgc cgagaatcgg gccggcttta
     1261 tgcctatccg atgcagggtc tgtacgccgt cggcgtggcc ggtgttgcgc tcgggatcgc
     1321 cggcgcaatg ctggatgcgt tcaaggaact ggcaacacaa aaggcgccgc gcaaccaggg
     1381 gcggttggcg gacaacactg tggtgcaggc gactgttgcg caaatgcagg cgcggctcga
     1441 tgcgtcgcgc gcctacctcg tggatacgct caccgcgatt tggtcggctg acgattcctg
     1501 ggtaatagac gtgccggcgc gcgccagggt acgcctcggc tgcacgttcg cgatccagaa
     1561 cgcggaggcg atcgcggatt atgcctacaa gtccgctggg gtggatgcga tcttcctcgg
     1621 caccgcgttc gagcggcggt ttcgcgacat gcacacgctg tcgcagcaaa tccaatcgcg
     1681 cgcagcgcat ttcgaggcgg tggggcagat actactcggc atcgacccgc ctggaacgtt
     1741 cttctaggca cgctacctcc agcccaagca cgcggcggat cgcattgcgc gtcattatca
     1801 gtggcgcgct ggcaggcagc tcccggacgc cgaagtggct ttgcggcagg gagcgggacg
     1861 cgggcatgcg cattacatga tcggtctcgc cgtcggcgcg cgccacgcca acctgcacgc
     1921 agacagcacg cagcacctgc tcagggatca gggacgacag tcccgccgtg gggtcgcacg
     1981 ccaatatgct atcctggata ttctgtgcgt tcacgggata cagcgcctgc atcacgccgg
     2041 acagcccacc gacccgcgta tagtcgcttg tatcgccgat gcggaacagc acgatcggac
     2101 tggcggtggc gcaggtggag gtatcgccgg cgctgatcat ataggtcccg tccatgccga
     2161 tcgcgtccgg cgtcagcacg ccgttgaata cgaacagaca caggcgatca tgcaatggct
     2221 cgatttccag gataacgtgc aggcgcggtc cgatggcgac ggcgatccct gcagcgctta
     2281 cctttccctc ggtgaacacg cagctcaggc cgtcggcgct gttccctatg tgcataaccg
     2341 acatgacgac agagccgcga ttctgtgtgg attggtagaa gccgccccat aatccgaggt
     2401 agttggcatc gatccgaggc tgccgctcgg cgcgcaggcc tgtgatcctc aggccctcgg
     2461 ccgcggccgg ctgaagcacc gcgggttcgg cacatgggtc cagcgcaggg cgttcccaaa
     2521 aatccagcgt gacctctgga cgataacgac ggacgatgtc ggtcaacctt gtcagattgt
     2581 gccctgtggg ctgattgacc ccgcccaacc acctgccgat gactgatttg tcgacgccga
     2641 gttcgcgcga cagcatgacc cggctgacat tgatgtcggc catcacctgc cgcagccggg
     2701 cggcaaagtc cggtcgatgt tcccccataa gcgaagctta tcggggtgtc acaattagtt
     2761 caattcggaa cggtcggttt gaacacgccg ccggaca
//
LOCUS       DATGEM010000553         8792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_881394, whole genome shotgun sequence.
ACCESSION   DATGEM010000553 DATGEM010000000
VERSION     DATGEM010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881394"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..553
                     /locus_tag="VKQ27_17010"
     CDS             221..553
                     /locus_tag="VKQ27_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90684.1"
                     /translation="MFTLPGVLRVEELKEPASLAVTRTGLDTRQDGSIFPLTLATPSS
                     EMAVPGYPPALILRISQPKDGSASLHIVVDMSSIHSLHGIVSTEEQIFGDEENDRTDA
                     SPGLASPP"
     gene            688..1521
                     /locus_tag="VKQ27_17015"
     CDS             688..1521
                     /locus_tag="VKQ27_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HLY90685.1"
                     /translation="MTAKTFLITGVSQGLGRAFAEAALAAGHMVVGTVRDAAARTAFE
                     ELGHGRAKAVILDVTDFAAVEAVIGQADRDAGGIDVLVNNAGYGHEGILEESPLDALL
                     HQFNVNVFGAVAMIKAVLPGMRARRRGHIINISSMAGLAALPGIAYYSGSKFALEGIS
                     EVLAKEVRDFGIHVTALAPGSFRTEWAGSSMVRSDRNIADYDALFAPVRRAREQKSGR
                     QSGDPAKAAKVLLDLVEAEHPPVHLILGADALQLVRDRTKALLAEFDAWEDVSRSTDF
                     D"
     gene            1591..2295
                     /locus_tag="VKQ27_17020"
     CDS             1591..2295
                     /locus_tag="VKQ27_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY90686.1"
                     /translation="MTDLSAFPISRQCPPLHPDRLQLYSHPTSNGVKVSIMLEEIGLP
                     YEAHLVHIGQSSTWTPEFLALNPNGKIPAIIDPDGPGGEPFALFETGAILLYLAEKTG
                     RLLPVDPSLRLETIQWLFFQMAAVGPIFGQLAYFHKFGGKEIEDKRPHERFRAETERL
                     LGVIDQRLAERPWMMGAEYTIADIALIGWVRNIVHTHGAGDVVGWERFTHARRWLDAA
                     IARPAVQRGLGIPSLP"
     gene            2409..3056
                     /locus_tag="VKQ27_17025"
     CDS             2409..3056
                     /locus_tag="VKQ27_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HLY90687.1"
                     /translation="MTKTLKIDFVSDVACPWCVIGLTALEQALERTKGEVEADIDFHA
                     FELNQAMAAGGQNMIQHVGEKYGISPEQARTNREKIRARAAELGFTMGTSDASRIYNT
                     FDAHRLLYWAKDQGKQLALKKNLFVANFTDQQDPGDHDVLIAAARKSGLDAETAREIL
                     SSDRFAEEVREEERYWQDRGISGVPAVIIENKYLVSGGQPAEIFEKALREVAAQS"
     gene            complement(3160..3618)
                     /locus_tag="VKQ27_17030"
     CDS             complement(3160..3618)
                     /locus_tag="VKQ27_17030"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HLY90688.1"
                     /translation="MLKNLEDYLYRNIPLSRAMCVSVLEISERSVLLGAPLAPNINHQ
                     ETAFGGSLSTLAILSAWSLVHTRLASADLPGRVVIQRNSFEYLEPVDGNFTARATLPE
                     PGDWPRFAHMLARRGKARLTVQAVLEQDGRVCGLFSGEFVALTAPAAPPI"
     gene            complement(3712..4560)
                     /locus_tag="VKQ27_17035"
     CDS             complement(3712..4560)
                     /locus_tag="VKQ27_17035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY90689.1"
                     /translation="MDVGVARTFLEIVKTGSFVNAATNLNLTQTAVSARIRVLEEQLQ
                     RPLFIRNKAGARLTPAGTHFLRFATTIVQAWDRARSTVALPVGKETVVTLGAELSLWN
                     PLLRHWLLWMRRECPAYSVHTRIASADELMDLVQDGTLDVAVLYAASRRLGVVSELLF
                     EEKLVLVRTRGVDRQLTPEDHVRVDWGDEFSELYQAAFPDHPSPVMSISYGPLALDYI
                     LAVGGSGYFRKGFVKPWLDDGRLEIVPDSPEFSYSAYAVHSAKADEGVMARIRAGLRE
                     AAAEAL"
     gene            5006..5401
                     /locus_tag="VKQ27_17040"
     CDS             5006..5401
                     /locus_tag="VKQ27_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90690.1"
                     /translation="MRRIISISVLALACLSAHAYASGPIGTVPDFRSIEDRLDGDDAL
                     AEGRTAIMGVIPPGTKVADAEAILKNAGALCKPKHHDAQTVRCIYNDVRIGDDAIDHV
                     QWNTLLHIVNDKVVGLSLNREVDRHSLQD"
     gene            5545..6054
                     /locus_tag="VKQ27_17045"
     CDS             5545..6054
                     /locus_tag="VKQ27_17045"
                     /inference="COORDINATES: protein motif:HMM:NF040052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HLY90691.1"
                     /translation="MNLRLSSSVCYADYADDIDMGDLGQDALIRSLGGLMLASKAMGR
                     AALMLIVDAEPEWHSFLARLHNMETRWSRQLRLAITGMDAALPPDVDGLHQRIVQHPD
                     PIDRFAAIRDELERAVAELDMLLPRIRHETLQESLRMMRADLRVSLAQAARSLAAKVG
                     DATSPAITD"
     gene            6058..8115
                     /locus_tag="VKQ27_17050"
     CDS             6058..8115
                     /locus_tag="VKQ27_17050"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HLY90692.1"
                     /translation="MPDHDQPLRLTNLVAVDGRKLLFSISNFLAAAATLVIAFEASLP
                     RPWWALLTVYVTAQPMSGAFRPKMLYRFGGIAIGAVVTIALVPNLQNSPILLVFCLAI
                     WTGFCIYLAVLDRTPRAFLFQMAAFSAAVMSFPYLDDPGDIFTTTVSRVEEMTVAILC
                     VTASHMLLQPWSVRPIIRERARAFLSHASTWTSEALGTHHTRLEYAHRRRLATDVVEL
                     GLMAIHLPFDQRLALATRDTVLEIQHRLAAVLSIASATADRLDRLRVSDGVDAEIAEI
                     VATLVAWLKDTDPRPDTSAATLLIARCRAAMDDRTQRDDWTSLLTASLCDRIAALVQA
                     LDEARQLVASFDGGEPRANIVDVRAASRFVLARDHGLAALAGAATATAICLYCAIWIL
                     LAWPTGSSTAAFAAIITCSFAAQDDPAPAIGRYLMATLSTFPLAALYLFVILPRADGI
                     GMLILTLAPTFIWMGYIQADPARSAKALPMFSAFIATTSFVDRFQADFAQFTNTSLAQ
                     VAGIATTLAVTRLFRSTSIRWTAYRIIRRNWRDLHNLADRREPLSSETWTARAVDRLG
                     QVAARMADADPGDTLHAADGLVDLRVGRNIIQVRRGIQRASERAQLAISPVLDGIAAL
                     FRSRLRSGLPVPADRKLLSAIDDAIRVGTDRASDMDAQSLLSLVGMRCNLFPTALPYG
                     ATG"
     gene            8115..8339
                     /locus_tag="VKQ27_17055"
     CDS             8115..8339
                     /locus_tag="VKQ27_17055"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HLY90693.1"
                     /translation="MIEEIHLFGIYVPAALFWAMLAVIITFLLRDLLVRLPLHRVLWH
                     PALLELAIFVVIWRALVWLGDRFLPHGLIS"
     gene            8385..>8792
                     /locus_tag="VKQ27_17060"
     CDS             8385..>8792
                     /locus_tag="VKQ27_17060"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HLY90694.1"
                     /translation="MIGVIVAIIYALWLRYQVEPLTRDGKVRADIVPVAADVGGLVTD
                     ILVHDNQQVKQGQVMLVIDRPRYQLALAQAEANLSNARVALSEAILEDRRNRAMPDVV
                     ARETTEQGTARVGQLRAAVQQAIVARDLAAFNLQ"
BASE COUNT         1584 a         2930 c         2622 g         1656 t
ORIGIN      
        1 ggcgcggtcg gtggcatcgc atggactcgg gcgcactggc cgggcgtgac cctgtacggc
       61 ggcgtcctgg ttctccttgc gctcgcgatc tcggtccgcc tggcaggcgt caagccgctg
      121 gagcctttgg cgcccacgct cgcgcccacc acgtcaagga cctgaaccgc ggggaccgat
      181 tggttgctgt accgtcagcc cctgtcggag caggcacagc gtgttcacgc ttccaggcgt
      241 tcttcgcgtt gaagagctga aggaaccggc ttctttggct gttacgcgaa caggtctgga
      301 caccagacag gatgggtcga tcttcccgct gacgctggcc actccgtcat ctgagatggc
      361 cgttcccggc tatccgcccg ctctcatctt gcgaatatcc cagccaaaag atggaagtgc
      421 gagccttcac atcgtcgtcg acatgtcgtc aattcattcg ctgcacggga tagtgtctac
      481 cgaagagcag atattcggcg acgaggaaaa cgatcgtact gatgcctcgc cgggtctcgc
      541 ttcgcccccc tgaagttgag gcccggtacc acgcgaccaa accggtagct tccttcaacc
      601 agaaagagag ctggctgtga acatcgtcga tccgattgcc gacgcagacc attcgcgtga
      661 cggcgggtca ttccgggagc ccaacgcatg accgccaaga ccttcctcat tacgggggtg
      721 agccaggggc ttggccgggc gttcgccgag gccgctctgg ccgccgggca tatggtcgtc
      781 ggcacggttc gcgacgccgc cgctcgcacg gcctttgaag agctcggaca tggccgcgcg
      841 aaggccgtga tccttgatgt aactgacttt gccgcggttg aggcggtcat cgggcaggcc
      901 gaccgggatg ccggcggaat cgatgtcctc gtcaacaatg ccggctacgg gcacgaggga
      961 atcctcgagg agtcaccgct cgatgcgttg ctccaccaat tcaacgtcaa tgtcttcggc
     1021 gcggtcgcca tgatcaaggc tgtgctgccc ggcatgcgcg cgcgccgtcg cggccacatc
     1081 atcaacatct cgtcgatggc gggcctcgcc gccctacccg gcatcgctta ttatagcggg
     1141 tccaagttcg cgctcgaagg catctcggaa gtactcgcca aggaagtgcg cgacttcggg
     1201 atccatgtga ccgcgcttgc gccgggctcg ttccgtaccg aatgggccgg ctcgtcgatg
     1261 gtccggtcgg atcgcaacat tgccgactat gatgcgcttt tcgcgcccgt ccgccgcgcc
     1321 cgcgagcaga agagcgggcg ccagtcggga gatccggcca aggccgccaa ggtgctgctc
     1381 gatctggtcg aggccgagca tccgccggtg catctgatcc tcggcgcgga tgcgctgcag
     1441 ctggtgcgcg accggacgaa ggcgctgctc gcggagtttg acgcctggga agacgtgtcg
     1501 cgctcgaccg acttcgactg acccctgatc tcgcctcatc atgacagcac cgccggcacg
     1561 aaccggagcg cggcggcagc ggagcacccc atgaccgatc tttctgcctt cccgatttcc
     1621 cggcaatgcc cgccgcttca tcccgaccgg ctgcagctct attcgcatcc gacatcgaac
     1681 ggcgtgaagg tgtcgatcat gctcgaggag atcggtctgc cttatgaggc gcatcttgtg
     1741 catatcggtc agagcagcac ctggacaccc gagtttctgg cgctcaaccc caatggcaag
     1801 atccccgcga tcatcgaccc cgatggcccg ggaggtgaac ccttcgcgct ctttgaaacg
     1861 ggtgcgatcc tgctgtatct cgccgagaag accggacggc tgctgcctgt cgatccgtca
     1921 ctccgcctgg aaacgatcca atggctgttc ttccagatgg ccgccgtggg cccgatcttc
     1981 ggccagctcg cctatttcca caaatttggc ggcaaggaga tcgaggacaa gcggccgcat
     2041 gaacgcttcc gcgccgaaac cgagcgtctg ctgggtgtca tcgatcaacg gctggcggag
     2101 cgcccatgga tgatgggtgc ggaatatacg atcgccgaca tcgcgctgat cggctgggtc
     2161 cgcaatatcg tccacaccca tggcgcgggc gacgtggtgg ggtgggagcg attcacccat
     2221 gcccgacgct ggctggacgc tgccatcgcc cgccctgccg tgcagcgtgg gcttggcatt
     2281 ccctccctcc cataaccgga gggcgagccg gccgctgcga ggaatatagt cgtccgtccc
     2341 gtggtaggat ggaaaaccgc cacgcaacgc tcatgtttga ccaacccgtc cacaaccgga
     2401 tgcatccgat gaccaaaact ctcaagatcg acttcgtttc cgatgtggcg tgcccctggt
     2461 gcgtcatcgg cttgactgcg ctcgagcagg cgcttgagcg cacgaaggga gaggtcgagg
     2521 cagacatcga ttttcacgcc tttgagctca accaggccat ggcggccggc gggcagaaca
     2581 tgatccagca cgtcggcgag aaatatggca tttctcccga gcaagcgcgc accaatcgcg
     2641 agaagatccg cgcccgggct gcggagctgg gctttaccat gggcacatcc gatgccagcc
     2701 gcatctacaa tacgtttgac gcccatcgcc ttctctactg ggccaaggat cagggaaagc
     2761 agctcgcgct caagaaaaat ctgttcgtcg cgaatttcac cgatcagcag gatccgggtg
     2821 atcatgatgt gctcatcgcc gccgcccgga aatcgggcct cgacgcagag accgcgagag
     2881 aaattctgtc gtccgaccga tttgcggaag aggtccgcga ggaggagcgt tactggcagg
     2941 acaggggcat cagcggcgtg ccggccgtga tcatcgagaa taaatatctg gtctccggag
     3001 gccagccagc cgagatcttc gaaaaggccc ttcgggaggt cgctgcccag tcatgaggca
     3061 gtggaagcct cccttcccct gatggggagg ctttaccccg gccgcgtcgc cggaccgatc
     3121 atggtgcgag gccgcccagc acggcgagca tgatctatcc tagatcggtg gtgcagccgg
     3181 tgcggtcagc gcaacgaact cgccggaaaa gaggccgcac acccttccat cctgctccag
     3241 caccgcctga actgtcagcc gggcctttcc ccgacgcgcg agcatgtggg caaagcgcgg
     3301 ccagtccccg ggctccggca gagtcgcacg ggcggtaaaa ttgccatcga ccggctcgag
     3361 atattcgaaa ctattgcgtt gaatgaccac gcgacccggc agatcggcac tcgccaggcg
     3421 cgtgtggacg agtgaccagg cagacaggat agccagcgtg gacaggctac ctccgaacgc
     3481 cgtttcctga tggttgatgt ttggagccag cggcgcgcca agaaggaccg agcgttcgct
     3541 tatctctaga acagaaacgc acatcgctcg agaaagagga atatttctgt agagatagtc
     3601 ttcaagattt ttgagcatca gaaacctatt ttctatccgc tgcgacaatc tgcggtccgg
     3661 attgtcggtg ccggtccgat tccaagcctg cggatatcat gatcgccacc gttaaagcgc
     3721 ctcggcggca gcttcccgca acccggcccg gatccgcgcc atcacaccct cgtccgcctt
     3781 ggccgagtgc accgcatagg ccgaatagga gaattccggg ctgtccggta cgatctccag
     3841 ccggccatca tccaaccatg gcttcacgaa gcccttgcga aaatatccgc tgcccccgac
     3901 cgcaaggata tagtccagcg cgagtggccc gtagctgatc gacatcaccg ggctcggatg
     3961 atccgggaac gcggcctgat aaagctcgga gaactcgtcc ccccaatcga cccggacatg
     4021 gtcttcgggc gtgagctgac gatcgacgcc gcgcgtgcgg acaaggacga gcttctcctc
     4081 gaacagcagc tcggacacaa caccgagacg gcgcgacgcc gcgtacaata ccgccacgtc
     4141 cagcgtcccg tcctggacga gatccatgag ctcgtccgcg ctcgcgatcc gcgtatggac
     4201 ggaataggcc gggcactctc gacgcatcca gagcagccag tgcctgagca gcgggttcca
     4261 gaggctgagc tcggccccga gtgtcaccac cgtctccttc cccaccggaa gcgccaccgt
     4321 gctccgcgcc cgatcccagg cctgcacgat cgtcgtcgcg aacctcagga agtgagtgcc
     4381 cgcaggagtg agccgggcac cggccttgtt gcggatgaac aagggacgct ggagctgctc
     4441 ctcgagcacg cggatgcgcg cgctgaccgc ggtctgggtg aggttgaggt tggtcgccgc
     4501 gttcacgaaa ctcccggtct tcacgatctc gaggaaggtc cgggcaacac caacgtccat
     4561 ctgcaacatt cctgcggtga agtatcaatc acatagaaat tcttgcatga tctaagctgc
     4621 tactgcctgc agaccaagca agaagtgctt tcacccctca cgtccgcgct tgaatccagg
     4681 aatcccgcaa aaccgggtta gcagactcga tttcagtgca attatcttgc cgaaaccgag
     4741 caattttttg cgctgtcgca ggcaggtcga catgcgtaac gaggtcctgg aaaggacatc
     4801 aacgaaaata tgactctaca aaaatcttaa tctccgcacc ggcgatctaa caatatctat
     4861 cgaccatatc agatttggcc cgaaatctta tttcgccgtt ccctatctgt cgtccgaacg
     4921 cgcccaatga tcgtggccgc acttgttgtt gcgcatgatg ccgatctcgc gacgcgtgtt
     4981 tcgacctcgt tgaaggagaa tatacatgcg tcgtatcatc agcataagtg tgctggctct
     5041 cgcctgtctt tctgcccatg catacgcttc ggggcccatc ggcaccgtgc cggatttccg
     5101 cagcatcgag gatcgtctgg atggggacga tgcgcttgcc gagggtcgga cggcgatcat
     5161 gggcgtgatc ccgcctggaa cgaaggtggc ggatgcggag gcgattctca agaacgccgg
     5221 cgcgctctgc aagccgaagc atcacgacgc gcagactgtg cgctgcatct acaatgatgt
     5281 tcgcattggc gatgacgcca tcgaccacgt ccaatggaac accctactcc acatagtcaa
     5341 cgacaaggtc gtcggcctgt ccttgaatcg ggaagtcgat cggcatagtc ttcaagactg
     5401 aaccgccgac gttggaagcc gggcgtgaag cccccttttc ccggcttttc ctctccggcg
     5461 tccataagaa caatggacgc cgggactttt ttgtctcccg cgccgctcga cccagcgtct
     5521 cggtgccgct cggagtacga acgcatgaac ctgcgcttgt cctcgtctgt gtgctacgcg
     5581 gactacgcgg acgatatcga catgggtgat ctcggccagg acgcgttgat ccggtcgctc
     5641 ggtggcctga tgctcgcatc gaaggcgatg ggccgcgccg cgctgatgct catcgtcgat
     5701 gccgagccgg aatggcactc gttccttgca aggcttcaca acatggaaac ccggtggagc
     5761 aggcagttgc gtctggcgat caccggcatg gacgcagctc tcccgccaga cgtcgatggc
     5821 ctccaccagc gcatcgtgca gcaccccgat ccgatcgacc gcttcgcggc gatccgcgac
     5881 gagctcgaga gggccgtcgc agagctggac atgcttctcc ccaggatccg ccacgaaacc
     5941 cttcaggaat cgctgcgcat gatgcgcgca gacctgcgcg tctccctcgc gcaagccgcc
     6001 cgatcgctgg cggcaaaagt gggcgacgcc acgtctcccg cgatcacgga ctagtcggtg
     6061 ccggaccacg accaacccct gcgcctcacc aatctggtgg cggtggatgg aagaaagctg
     6121 ctgttctcga tcagcaactt ccttgctgct gccgctacgc tggtgatcgc cttcgaggcc
     6181 agcctgccgc gtccatggtg ggcgctgctc acggtgtatg tgaccgcgca acccatgtcg
     6241 ggcgccttcc gacccaagat gctgtatcgg ttcggcggca tcgcgatcgg cgccgtcgtc
     6301 accatcgcgc tcgtacccaa tctccagaac tcgccgatcc tgctggtgtt ctgcctcgcg
     6361 atctggaccg gcttctgcat ctatctcgcg gtgctcgatc gcaccccgcg cgcctttctg
     6421 ttccagatgg cggccttcag tgcagccgtg atgagtttcc cctatctcga cgatcccggg
     6481 gacatcttca ccaccacggt gtcgcgcgtg gaggagatga ccgtcgcgat tctctgcgtc
     6541 acggcgagcc atatgctgct ccaaccctgg agcgtccggc caatcatccg cgagcgcgcg
     6601 cgggccttcc tctcccatgc gtcgacatgg acatcggaag cgctcggcac acaccatact
     6661 aggctcgaat atgcgcatcg ccgacggctg gcgactgatg tcgtcgagct tggcctgatg
     6721 gcgatccacc tgcctttcga ccagcggctg gcgctggcga cacgcgacac ggtgctggag
     6781 atccagcacc gcctggctgc cgttctctcc atcgcctctg ccaccgcgga tcgtctcgac
     6841 cggctgcggg tctcggacgg ggtcgatgcc gagatcgccg aaatcgtggc gaccctggtc
     6901 gcctggttga aagatacgga cccacggccg gatacatcgg cggcgacgct cctcatcgcg
     6961 cgctgccgtg ccgccatgga cgaccgcaca cagcgggacg actggacgtc cctgctcact
     7021 gccagcctct gcgatcgcat cgccgctctg gtgcaggcgc tcgacgaggc caggcagttg
     7081 gtcgcctcgt tcgacggcgg ggagccacgg gccaacatcg tcgacgtccg cgccgcgtcg
     7141 cggttcgtgc tggcccgcga ccatggactt gcagcgctcg cgggggctgc caccgctact
     7201 gccatctgcc tctactgtgc gatctggatt cttctcgcct ggcccaccgg ctcctcgacc
     7261 gccgcctttg ccgcgattat cacctgttcc ttcgcggccc aggacgaccc ggcgccagcg
     7321 atcggccgct accttatggc gaccctgagc acatttcccc tcgccgcgct gtatctcttc
     7381 gtgatcctgc cgcgcgccga cgggatcggc atgctgatcc tgacactcgc gccgacattc
     7441 atctggatgg gatatatcca agccgatcca gcgcgttcgg cgaaggcgtt gccgatgttc
     7501 tccgccttca tcgcgacgac gagcttcgtc gaccgctttc aggcggactt cgcccaattc
     7561 accaacacca gcctcgccca ggtcgcaggc atcgccacca ccttggccgt cacccgacta
     7621 ttccgttcca cgagcatccg ctggacagcc taccggatca tccgccgcaa ctggcgcgat
     7681 ctccacaatc tggcagaccg acgcgagccg ctttcctccg aaacctggac ggcccgtgcc
     7741 gtcgaccggc tcggccaggt cgccgcacgc atggccgacg cggatcccgg cgacacgctc
     7801 cacgccgccg acggccttgt ggacctccgc gtgggccgca acatcataca ggtccgtcgc
     7861 ggtatccagc gggcgtccga acgcgcgcag ctcgccatct cgcccgtgct cgacggcatc
     7921 gccgcgctct tccgatcgcg gctccgcagc gggcttcccg tcccggcgga ccgcaagttg
     7981 ctgtccgcca ttgacgacgc gatcagggtc ggaacggacc gggcctcgga catggatgcc
     8041 cagtcgctgc tctcgctcgt cggcatgcgc tgcaacctct tcccgacggc ccttccctac
     8101 ggagcgaccg ggtgatgatc gaggagatcc accttttcgg catctacgtg cccgctgcgc
     8161 tgttctgggc gatgctcgcg gtgatcatca ccttcctgct ccgcgatctg ctggtgcggc
     8221 taccccttca ccgggtgctc tggcatcctg ccctgctcga gctcgccatc tttgtggtga
     8281 tctggcgggc acttgtgtgg ctcggcgatc ggttccttcc acatgggctg atttcctgaa
     8341 tgttccgact gcacaatctc gtgcggaccc tcttcacggt cctgatgatc ggcgtcatcg
     8401 tcgcgatcat ctatgcgctc tggctgcgct accaggtcga gccgctgacg cgggacggca
     8461 aggtcagagc tgacatcgtg ccggtcgccg ccgatgtcgg cgggctcgtc accgatatac
     8521 tggtgcacga caaccagcag gtgaaacagg gccaggtcat gctggtcatc gaccggccgc
     8581 gctaccagct cgcgcttgca caggccgagg ccaacctctc caacgcgcgc gtcgcgctca
     8641 gcgaagcaat cctggaggat cggcgcaacc gcgcgatgcc ggatgtcgtc gcccgcgaga
     8701 ccaccgagca gggcaccgcc cgggtcggac agcttcgcgc cgcggtccag caagcgatcg
     8761 tcgcgcgcga tctcgccgcc ttcaacctgc aa
//
LOCUS       DATGEM010000554         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_492926, whole genome shotgun sequence.
ACCESSION   DATGEM010000554 DATGEM010000000
VERSION     DATGEM010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492926"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VKQ27_17065"
     CDS             complement(<1..891)
                     /locus_tag="VKQ27_17065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90695.1"
                     /translation="MHRRTLLKAAATTLAGTLAAPAIAQGDAKVLRCAPEANLASIDP
                     IWTTATVAINHGYMVYDTLYGTDLSLTPRPQMAAGHTVSDDKRTWTFTLREGLAFHDG
                     EPVRAADCIQSIDRWSKRAAWGQALGAATEEMKVVNDKTFVIQLKKPFSSILYALGVS
                     GCFIMPERVAKTDPFKQITDATGSGPFKFVKDEWVAGAKAVWVKNDKYVPRNEPADFY
                     AGGKRVFVDRVEHVVMPDGATAAAALQTGEVDWVHVPQIDLVPMLKKSPGVTTAVYDP
                     LGWLGIIRPNFLFPPFDNRKL"
     gene            1177..1992
                     /locus_tag="VKQ27_17070"
     CDS             1177..1992
                     /locus_tag="VKQ27_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY90696.1"
                     /translation="MPTPDKSGMAPVNGISMYYAIYGHGPPLLLIHGGLSSSDVWAGV
                     IPLLDRQHEVIVADSRGQGRSTQTSERLTYGLMANDYLALLDYLNVNQVALVGWSDGG
                     NIGLDLAIHHPDRLTALFSQAADASPDGLVAFSQPQKQDFLSRLKHYAGAAKRRIDTI
                     FDIGKSRLLAMFGYHESGPPPPTVALQREIFQLWATEPHYTEQQLASIRVRTAIVSGD
                     HDNAVRLDHTKYLARTIPGAQLIILPDVGHAAMLQDPAGYAQAVLRFVDGPAD"
BASE COUNT          382 a          725 c          628 g          412 t
ORIGIN      
        1 cagcttgcga ttgtcgaatg gcggaaacag gaagttcggc cggatgatgc caagccagcc
       61 gagcggatcg tacaccgcgg tcgtgacgcc gggactcttc ttcagcatcg gcaccagatc
      121 gatctgcggc acatgcaccc aatcgacttc accggtctgc agcgccgcgg cagccgtcgc
      181 gccatccggc atcacaacgt gctccacgcg atcgacaaac acgcgcttgc cgccggcata
      241 gaaatccgcc ggttcgttgc gcggaacgta tttgtcgttc ttcacccaga ccgccttggc
      301 cccggcgacc cactcgtcct tcacaaactt gaatgggccg gaaccggtcg catcggtgat
      361 ctgcttgaac ggatccgtct tggcgacccg ctcaggcatg atgaagcatc cggacacgcc
      421 aagcgcatac aggatcgacg agaacggctt cttcaattga atgacgaagg tcttgtcgtt
      481 caccaccttc atctcttccg tggccgcgcc cagcgcctgc ccccacgccg cgcgcttcga
      541 ccatcggtcg atcgactgga tgcaatcggc cgcgcgcacc ggctcgccat catggaatgc
      601 gagcccttca cgcagcgtga acgtccatgt ccgcttgtcg tccgagacgg tgtgtccggc
      661 ggccatctgc gggcgcggcg tcagcgacag gtccgtgccg tacagcgtgt catagaccat
      721 gtagccgtgg ttgatcgcga cggtggcagt ggtccatatc ggatcgatac tggcgaggtt
      781 cgcctcgggc gcacagcgca gcaccttggc gtcgccctgc gcgatggctg gtgcggcgag
      841 tgtgccggcc agtgtggtgg cagcggcctt gagcagcgtc ctacgatgca taccttgtcc
      901 cccaatgtgg ttgatggacg tttgcccgat tcttgtgatt gcagacatgt cgcaggcggc
      961 acggactatg cggcctcaac tccggcaaga tcgcctaacc gtgcgttcgc tgcaacaaga
     1021 cacatggatg tggcttgtcc tgctgcctgc gcgagcgcac ggtgtgccac gatgacctgg
     1081 cgcttgtatc tgctggcgct ctgcttcctt gtcctggtat ttggcgcacg gacctatgcc
     1141 gcgccacgat ggcagaccga cccatatcct ccgtctatgc cgacgccgga caaaagcggc
     1201 atggctccgg tcaacggcat ctcgatgtac tacgctatct atggtcatgg gccaccgctc
     1261 ctgctcatcc acggaggctt gagcagctcc gacgtgtggg caggcgtgat accgctgctg
     1321 gacaggcagc atgaggtcat cgtcgcggac agccgcggtc agggtcgttc gacgcagacc
     1381 agcgagcgat tgacttacgg tctgatggcc aacgactacc tcgccttgct cgattacctc
     1441 aacgtgaacc aggtggccct cgtcggttgg agcgacggcg gcaacatcgg tctcgatctc
     1501 gccatccatc accctgatcg cctgaccgcg ctgttctcgc aggccgccga tgcaagtccc
     1561 gacggcctgg tcgcgttttc gcagccgcag aagcaggatt ttctgtcccg cctcaagcat
     1621 tatgccggtg cggcaaagcg gcggatcgat accatcttcg acatcggcaa atcacgcctc
     1681 ctcgccatgt tcggctacca cgaatccgga ccgccccctc caaccgtggc tttgcaacgg
     1741 gagatcttcc agctgtgggc gaccgagccg cattacaccg agcagcaact ggcatcgatc
     1801 cgcgtgcgca ccgcgatcgt gagcggcgac catgacaacg cggtgcggct cgatcacacg
     1861 aagtatctgg cccgcacgat ccccggcgcc cagctcatca tcctgcccga cgtcggccac
     1921 gccgccatgc tccaggatcc agccggctac gcccaggcgg tcttgcgttt tgtggacggc
     1981 ccagctgact gatcgtctta ccgcttgcgc accgcatcgc tcatccggcc aacgatctcc
     2041 ttcagcaccg gcgcgggtgt ggcgaaattc aaccgcgcga acgccgcata ccgctcgccg
     2101 aacgtttcgc ccgcgttaag ccccacgcgc gcgctgtcca ggaagaa
//
LOCUS       DATGEM010000555         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_377214, whole genome shotgun sequence.
ACCESSION   DATGEM010000555 DATGEM010000000
VERSION     DATGEM010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377214"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..463)
                     /locus_tag="VKQ27_17075"
     CDS             complement(260..463)
                     /locus_tag="VKQ27_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90697.1"
                     /translation="MAKISDSKLIELAKRLFDEALASDTLKAPRAIGILSCNSNRRRV
                     AVSATRATWRDYIPDAKKILSGI"
     gene            complement(715..1626)
                     /locus_tag="VKQ27_17080"
     CDS             complement(715..1626)
                     /locus_tag="VKQ27_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLY90698.1"
                     /translation="MTTDSVAPTPLEQERAAEELEATGNFRVLRRLLSRSQYAIDDGS
                     AKKIGIVLDVETTGLDTATDEIIELGMVKFEFATDGRIFRILDRFDELQEPSIPISME
                     ITALTGITAGDVAGKAIDPVAVEAFVEDAAVVIAHNAGFDRKFCERFWPGFVHKGWAC
                     SVTQIDWRAEGFEGSRLGYLLSGCGLFHDGHRAQTDCEALLEILSRPLPKSGEPALKR
                     LLDVARRTSARIWAENSPFDMKDVLKGRGYRWNDGSDGRPKAWWHDVPEDERETEFTF
                     LRDEIYLRDVEPNWSRISTFERFSTRG"
     gene            complement(1648..5079)
                     /locus_tag="VKQ27_17085"
     CDS             complement(1648..5079)
                     /locus_tag="VKQ27_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLY90699.1"
                     /translation="MFDSVTRDFIATAPGLPDLPAGQFAAALNEAYVEIATARLARID
                     NPQVVPQTLIATLNRLNRMADTLEAHVVLGISGDKARAAAFVAASARQAAAQIRRIMV
                     GQHQTLLDVDQVSGDLTSSLLFLIAERSADAFEAARPINAAPLEGVRQQLAIAIANLA
                     RGNLSAIDVINPELVEPEPGLDDQAVGDLLYYQILQGVILAAQDCLGTIDFPSLNAAQ
                     AIFERVLELSTLTSTTEAIEGAAPPDHIYRSASLYAGPHHLAGLLKRAAGTLQTTATI
                     RLTPPEGADGDMWRDWLLREASKIPFIWENHQRAIATGYLNVGSSLVMTTPTGSGKST
                     LSVLKIASTICNRRTVVYLAPTRALVSQVQADLEDRLAGLSIPVNIEDLLLEELGGEA
                     LPQIAVLTPESCLAYLAFAPELFANVGLLVFDECHLLSAPPQDIVVDGRVLQDPVDRR
                     SIDANLCLLNFAKAAPEADYLLLSAMVSNGEELAQWVENSLARGRRCFPFDDKWKPTR
                     QVKATVVYMQDEIDAAQEALDAHSSSVRKLPARPPKAVMDHVRALPYGLFSTSAGWNP
                     DAEGALAVRPFLQVSAQLGANPGRATILLGRRQVSYPKWKISANRLEIAAEIAAAYAK
                     CGLNVIVFCETTPWTASVAKNVSDLVGPVEVVLNENQAAWRHKALQEAGSEAAIYDAG
                     SGVAAVHHGDLLPEERFLAESVFRESTGVKVLSATSTLAQGLNLPCDVVIMAGTDRLD
                     DDDDRARRVPLFAHEILNALGRAGRARQAATGLGIVVPADPVVCDIVGANRIDNKSLL
                     PTVFSDGDNCIAITDPITLLYDRIVVGARVEPQAKYLLRRLAPSFGAARDGIEGFAEL
                     TRRSFGYFVKARRDPALAEFWLVDRQNSLAQLVEPPPVAPEQDWTVALAAKTGASPEL
                     VAKIADALRDHAPAIASTTEWMLWLLDLLETDQADFDMFLRPDAVERVFARGYTSAAE
                     GPQARARAREAVRAILPLWMETKTLIELEQAIVDFIRQHEVATTPTRAAAKATHARRF
                     VLRLAPDLNYLAGLLAQIAKVTAQQRGEEPLAFTQMLPQLVKRGFETIYHFRISRDID
                     DISRQDVAQIYSNISAHLLVDAADNWETVTEKVERAQELNSIEVHNNFVLQPPEE"
     gene            complement(5069..>5437)
                     /locus_tag="VKQ27_17090"
     CDS             complement(5069..>5437)
                     /locus_tag="VKQ27_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90700.1"
                     /translation="LITRVWREFAEIDADTRIDEMFAAITALLGINEVRAADAEQALN
                     SILHNRNMRYRVAVTAPVTAARANSYAHLFAGYELAIPHHLNARMAEFIALPDVRGFL
                     NQLAIDVIAALDEMERTYVR"
BASE COUNT         1000 a         1637 c         1768 g         1032 t
ORIGIN      
        1 cattttaagg gcgtgcaggt ccttcacgat tcaacccata gcgcgtcgct ggacaagaag
       61 gccgatgctg tcccgccaca aggaaggcgc gcgctggggt gcctgctgcg gctgagagat
      121 ggttccctac accccatcac ctaaatgcga aactgagccg agggtcgaaa tgtccctcgg
      181 ctcagtcgct ttagggaggc ccggcgttat tttagggagg cccggcgtta ttcgccgggc
      241 ctccccgtgg ccgacgatat cagatcccag aaaggatctt tttagcgtcg gggatgtagt
      301 cccgccaagt ggcgcgggtc gcactcaccg ccacacgtcg cctgttgctg ttgcaggaga
      361 gtatgccgat tgcccgcggc gcctttaagg tgtcgctggc gagtgcctca tcgaacaggc
      421 gcttcgcgag ttcgatgagt ttactatcgc tgatcttggc catgagagcc tccttcagcc
      481 ggtaagttga gggaaaacgc ccacaaatga tgccaccggc aaggctccct cgtgggagct
      541 ttccggttaa cctccggcgc cggagggcgg gacactggga aatgccaggt tggcggtgca
      601 gcccgcgcgc gggtgcgatg ccataaaagc tgatccaata ttatcgaccg gttcgatcaa
      661 aatcaagctt gagacgagaa tttgtccggc tcggggtctt attgtgagct cgagtcagcc
      721 cctagttgaa aatcgctcga acgtcgagat ccggctccag ttgggctcga catcgcgcag
      781 atagatctcg tcgcgcagaa aggtgaattc ggtctctcgt tcgtcttcgg gcacgtcgtg
      841 ccaccaggcc ttcgggcggc cgtcgctccc atcgttccat cgatagccgc ggcccttcag
      901 cacgtccttc atgtcgaagg ggctgttctc cgcccagatg cgtgccgagg ttcggcgggc
      961 gacatcgagt agccgcttca gcgccggctc gccggatttc ggcagcgggc gggagaggat
     1021 ctcgaggagc gcctcgcaat cggtctgggc ccgatggccg tcatggaaga ggccgcagcc
     1081 actcagcaga tatcccaacc ggctgccctc gaagccctca gcgcgccagt cgatctgggt
     1141 caccgagcag gcccatccct tatgcacgaa gcctggccag aagcgctcgc agaacttccg
     1201 gtcgaacccg gcattgtgtg cgatgacgac cgcggcatcc tcgacgaatg cctcgacggc
     1261 aaccggatcg atggctttgc ctgccacgtc gcccgccgtg atgccggtga gcgccgtgat
     1321 ctccatcgag atgggtatac tcggttcctg caactcgtca aatcgatcaa ggatgcgaaa
     1381 gatccgaccg tcggtggcga actcgaattt taccatgccg agctcgatga tctcgtccgt
     1441 ggcggtatcg aggcccgtcg tttcgacgtc gaggacaatg ccgatcttct tggcgctgcc
     1501 atcgtcgatc gcgtactggc tgcggctcag caaccggcgc aggacccgga aattaccggt
     1561 cgcttcaagc tcttccgccg cgcgctcctg ctccaggggc gtcggcgcta cagaatcggt
     1621 cgtcaaaaat cctccagctc tacccgatca ttcttcagga ggttgaagca cgaagttgtt
     1681 atgaacctca atgctgttga gttcctgggc tcgttctacc ttctcggtca ccgtctccca
     1741 attatcggcg gcatccacca gcaggtgagc gctgatgttg ctgtagatct gggcgacgtc
     1801 ctggcgcgaa atgtcgtcga tatcgcggct aatacggaaa tgatagatgg tctcgaagcc
     1861 gcgcttcacg agctgcggta gcatctgtgt gaaggcaagc ggctcctcgc cgcgctgctg
     1921 ggccgtcact ttcgcgatct gagccagcag tccggccaaa taattgagat cgggtgccag
     1981 acggagcacg aagcggcgag catgggtggc cttcgccgca gccctggttg gagtcgtggc
     2041 gacctcatgt tggcggatga agtccacgat cgcctgctcg agttcgatca gggtcttggt
     2101 ttccatccat aggggaagga ttgcccgcac cgcctctcga gcacgggcac gcgcctgcgg
     2161 tccttccgca gcggaggtat atccgcgggc gaagacgcgc tcgaccgcat ccggcctgag
     2221 gaacatatcg aaatccgcct ggtcggtttc cagaaggtcg agcagccaga gcatccactc
     2281 ggttgtgctg gctatggccg gcgcgtggtc tcgcagcgcg tcggcgattt tggcgacaag
     2341 ttcgggactg gcgccggttt tagcggctag tgccaccgtc cagtcttgtt ccggcgcgac
     2401 tggtggcggc tcgacaagct gcgccaggct attttgccga tcaaccagcc agaattcggc
     2461 cagggccgga tctcgccgcg ctttcacgaa gtagccgaac gaccgccggg tcagctcggc
     2521 gaatccctcg attccgtcgc gggcggcgcc aaagctcggg gccaagcgcc gcagcaggta
     2581 cttggcctga ggttcgacgc gcgccccgac cacgatgcgg tcgtagagaa gtgtgatcgg
     2641 gtcggtgatg gcgatgcagt tatcgccgtc agagaagacg gtcggcagga gggatttatt
     2701 gtcgatcctg ttcgcgccga cgatatcgca gacgacagga tcggcgggca cgacgatgcc
     2761 aaggcctgta gctgcctggc gcgcgcgtcc agcccgaccg agggcattaa ggatctcatg
     2821 cgcaaagagc ggcacgcgcc tggcccgatc gtcatcgtca tcgagccggt cggtgccggc
     2881 catgatcacg acatcgcagg gaagatttag gccctgcgcc aaggtcgagg tggctgaaag
     2941 aaccttgacg ccggtggact cgcggaaaac cgactcggcc aagaaccgct cctcgggcag
     3001 caggtcacca tgatgcacgg cggcgacgcc gctgcccgcg tcatagatgg cagcctcgga
     3061 gccggcttcc tgcagcgcct tatggcgcca cgccgcctga ttctcgttga gcacgacttc
     3121 gacggggccg acaaggtcgg acacattctt cgcgaccgac gccgtccaag gtgtggtttc
     3181 gcagaaaacg atcacattca atccgcattt cgcataagcc gcggcgatct cggccgcaat
     3241 ctcaaggcgg ttggccgaga ttttccattt agggtacgag acttgtctgc ggcccagaag
     3301 gatggtcgcc cgcccggggt tggcgccgag ctgggcgcta acttgcagaa agggtcggac
     3361 cgccagggct ccctcggcgt ccggattcca gccggccgat gtcgagaaca gaccataggg
     3421 gagggcccgg acgtgatcca ttactgcctt cggcggcctc gccggcaatt tcctcaccga
     3481 actcgaatgc gcgtcgaggg cttcctgtgc cgcatcgatc tcgtcctgca tgtagacgac
     3541 ggtcgctttg acctggcggg tcggcttcca cttatcgtca aaggggaagc agcggcggcc
     3601 gcgcgcgagg ctgttctcga cccattgcgc cagctcctcg ccgttgctga ccatcgctga
     3661 tagcagaaga tagtccgcct ctggcgccgc cttggcgaag ttcagcaggc agaggttggc
     3721 gtcgatgctg cgacgatcga ccggatcttg cagcaccctg ccgtcgacca cgatgtcctg
     3781 cggcggtgcc gagaggagat ggcactcgtc gaacaccagc aggccgacat tggcgaacag
     3841 ctctggggcg aaggcgagat atgccaggca gctttccggc gtcagcacgg cgatctgggg
     3901 cagcgcctcg ccgccgagtt cctcgagcag cagatcctcg atattgaccg ggatcgagag
     3961 gccggccagc cggtcttcca gatcggcctg gacctgggag actaaggcgc gggtcggcgc
     4021 gagatacacg accgttcggc ggttgcagat ggtcgacgca atcttgagga ccgacagcgt
     4081 gctcttacct gaaccggtcg gcgtcgtcat caccagcgac gagccgacgt tcaggtagcc
     4141 ggtggcgatg gcacgctgat ggttctccca gatgaacgga attttcgaag cctcgcgcag
     4201 cagccaatcg cgccacatat cgccgtccgc gccctcgggc ggggtcaacc ggatggtcgc
     4261 ggttgtctgc aaggtgccgg cggcgcgctt gagcagaccg gcgagatgat gcggcccagc
     4321 atagagggat gcggatcggt agatgtggtc gggaggggcg gcgccctcga tggcctcggt
     4381 cgtgctcgtc agcgtcgata gctcgaggac acgctcgaaa atggcctgtg ccgcattgag
     4441 gctgggaaaa tcgattgtgc cgagacaatc ctgggccgcg agaatgacgc cttgcaggat
     4501 ctggtaatag agaaggtcgc cgaccgcctg gtcatcgagc cccggctccg gctccaccag
     4561 ctcgggattg atgacgtcga tggcactcag attgccacgg gcgagattgg cgatggcaat
     4621 ggccaactgc tggcggactc cctccagagg agccgcgttg ataggccgcg cggcttcgaa
     4681 agcgtcggcg ctacgctctg caatgaggaa gagcaacgag gacgtcaggt ctccgctgac
     4741 ctggtcgacg tccagcaagg tctggtgttg gccgaccatg atgcggcgga tctgcgccgc
     4801 cgcctgccgg gcgctggcag cgacgaaggc ggcggctcgg gctttgtcgc ccgatatccc
     4861 gagaacgaca tgggcttcaa gggtgtcggc catccgattg aggcggttca gcgtcgcgat
     4921 cagagtctga ggcacgactt gcgggttgtc gatccgggcg agccgagctg ttgcaatctc
     4981 gacgtaggcc tcgttcaggg ccgccgcgaa ttgaccggcc gggagatctg gcaggccggg
     5041 cgcggtcgcg atgaagtcgc gggtgacgct atcgaacata agtgcgctcc atctcgtcga
     5101 gggcggcgat gacgtcgatg gcgagttgat tcagaaaacc gcggacatct ggcaaagcga
     5161 tgaattccgc catgcgggca ttgaggtgat gaggaatggc cagttcatat cccgcgaaga
     5221 ggtgggcgta gctattcgcc ctggcggcgg tcacgggagc cgtgacggca acgcgatatc
     5281 gcatattccg gttgtgcaga atgctgttca gcgcctgctc agcatcggca gccctgacct
     5341 cattgatgcc gagcagcgcg gtgatcgccg caaacatctc gtcgatccgc gtgtcggcgt
     5401 cgatctcagc gaattcgcgc cacacgcggg tgatcag
//
LOCUS       DATGEM010000556         7563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_901585, whole genome shotgun sequence.
ACCESSION   DATGEM010000556 DATGEM010000000
VERSION     DATGEM010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901585"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VKQ27_17095"
     CDS             complement(<1..748)
                     /locus_tag="VKQ27_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLY90701.1"
                     /translation="MPVTTPMVAMRDSIRLATDVYLPDGAGPFPVIMERTPYGRNIAS
                     RSEITAAQPTPVSRAELAAYFTAHGYAVVFQDTRGRYGSEGRFVKYLSDGEDGFDTCA
                     WLRDQTWCDGRICTMGLSYAAHTQAALACLDPPGLVAQVLDCGGFVNSWQSGIRQSGA
                     FELKQATWAFKNALVSPEAQADPVLHAALAAEDICEWFTRMPWKPGHSPLRHHPDYEN
                     YLFDQWTHGAFDPFWQQLGIWAEGSYHRYSR"
     gene            complement(793..3003)
                     /locus_tag="VKQ27_17100"
     CDS             complement(793..3003)
                     /locus_tag="VKQ27_17100"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY90702.1"
                     /translation="MAARALMAPRGGMPRTRRSIGGRGALWVIWALVMIMLVGTGYAA
                     VEYLRTSALSSARRDMANVSIALAEQTARTIQSVDVVLRDFQTHTAQLDVRTPEQFRE
                     QMAGEGVQRLLAGRLRNLPQAEALALVDAQGMLLNWSRDTPVQQIDLSDRDYYRHLRG
                     HAGAGVYVSGPKLGRTSHKPLLLIVRRIDAPDGTMLGLVAGVIDAKYLEDFYQKVSMQ
                     PGQAVALLNHDGVVIAGYPELGDWRGKRMPAGSAWYQQVEQGGGTYDAADYLGDPGAI
                     VAVRPLSDLPLIVDVSVTTQAALASWHRDAFDVAIIRVGVALVFTALFFAISWLFRQQ
                     ESHARELARVAERLRASERQVRDFAEISSDWFWEQDADLRFVWLSQTALLNAGGRRSY
                     VGLTRWELAGSDGTDPPWPEHIAELAARKPFRDFRFQFRAADGSGRYISASGLPIYDE
                     AGTFIGYRGTACDVTAQVQVEEELRRSKDAAEAASRAKSEFLANMSHELRTPLNAIIG
                     FSELMQKQQRSGRAGDVYLEWAGDIMASGRHLLDVINEVLELARIEAGRYSLAQDRVD
                     LGSAVRACLAMVKLQAEAKRVGVECSIAEREILLRADARAVRQIVLNLLTNAVKFTPS
                     GGTVSIRGGRAANGDVMLAVADTGIGIDSAALPSLGQPFTQADASISRTYGGTGLGLS
                     ISRKLLAMHGGKLTIESRPGQGTTVRVIFPSIRVLAGGHVPALVSPSLEPTVTD"
     gene            complement(3091..3861)
                     /locus_tag="VKQ27_17105"
     CDS             complement(3091..3861)
                     /locus_tag="VKQ27_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90703.1"
                     /translation="MIEVAALHVRFAEIEAVKDVSFEVARGESFGLVGESGSGKSTVL
                     RALSGLNADWTGRVVIDGDIMAPRRGKDFHRRVQMVFQDPYGSLHPRQTVDRLLSEPL
                     LVHRMDNIERRIMRALNEVALSPEVRFRYPHQLSGGQRQRVAIARALMAEPEVLLLDE
                     PTSALDVSVQAEVLNLLSDLQQARGLTYILVSHNLAVVAHLCDRIGVMQGGELVELLS
                     ADDLRAGRTKHAHTAALRELSIELEEPEAETLSGFQRK"
     gene            complement(3858..4688)
                     /locus_tag="VKQ27_17110"
     CDS             complement(3858..4688)
                     /locus_tag="VKQ27_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90704.1"
                     /translation="MLVEIQNLRIQFPTPTGLSDAVRNVSLTLQQEKLGIVGESGSGK
                     SLTARSILRLLPSYAITTADTLTFDGIDVLRANEATLRSIRGTRAGLILQDPKYSLNP
                     VMTAGAQIAEAWRVHRGGSRKVAEKAAIDLLAQVQIRDPARVAQAYPHELSGGMGQRV
                     MIAMMLAPDPELLIADEPTSALDATVQAEILRLIEDLVSRRGMGLILISHDLPLVSHF
                     CDRVAVMYAGRIVEELRASELTSARHPYTRGLLECLPTLSHKQTRLAVMQRDPSWLGE
                     "
     gene            complement(4823..5722)
                     /locus_tag="VKQ27_17115"
     CDS             complement(4823..5722)
                     /locus_tag="VKQ27_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90705.1"
                     /translation="MIALSRDWLLSEAPASRAHAAWVRRYRGWLQFRSNHLAMVGLVT
                     IVLMIVVSLAAPLIAPQDPSAQDLANRLAPPSWEHLLGTDELGRDLFARVIYGGRITL
                     GMVVAIVLLVAPIGLAVGSIAGYLGGLADRALMRVTDVFLAFPRLVLALAFVAALKPG
                     ITSAIIAIALTAWPPYARLARADTLTVRHTDYIAAVRLTGASPGRIILRHIIPLCLTS
                     VIVRVTLDMSGIILTAAGLGFLGMGAQPPLPEWGTMIAASRAFILEQWWVPTIPGVAI
                     FVASLAFNLLGDGMRDVLDPRQR"
     gene            complement(5719..6765)
                     /locus_tag="VKQ27_17120"
     CDS             complement(5719..6765)
                     /locus_tag="VKQ27_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90706.1"
                     /translation="MSATTQTTRWRFPTRLHPAVSAIASVPVTLFGLLLVTFLIGRVM
                     PIDPVIAIVGDHAPPDVIARVRTELGLDRPLYVQFGIYLSNLAHGDLGTSVMTTRPVT
                     ADIARFFPATLELATAAIIIAILVGVPLGVFAAARQGSRFDHVVRVISLAGQSVPVFV
                     LGLICLLVFYVKLGIAPGTGQNDVGFEGMVPRVTGMLVIDAALANDWDSFWDALAHLA
                     QPALVLAYFSMGYITRMTRAFMIEALSGEYIITARAKGLSPARILWKHAFGNMAVRLV
                     TVLALAYASLLEGAVITETVFSWPGIGLYLTVSLLNADMNAVLGATLVIGMVYVVLNL
                     LSDMAYRLLDPRVQ"
     gene            complement(6762..>7563)
                     /locus_tag="VKQ27_17125"
     CDS             complement(6762..>7563)
                     /locus_tag="VKQ27_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90707.1"
                     /translation="DQLKSVVGQSEFSVAREPQLTSMYLAANMGQPMFQKVQVLQALK
                     WAIDYEAIAKNITPNVWNVWQSVLPKGVPGAISDTPFKKDVAKAKALLAQAGYPDGFS
                     VTLDHFSNAPYSVIAQAIQADLAAVGIKAQLLAGEHKQVISKMRARQHQLVLQTWFPD
                     YLDANSNMQAFAANPDDGDNSKLKILAWRCHFFDKELTAGVEEAAKELDTAKRMAMYA
                     KMQRAFMERAPFVMLLQNAEVDVMRKGVAGIQIGVLPDYTRYAAITKA"
BASE COUNT         1414 a         2701 c         2224 g         1224 t
ORIGIN      
        1 cgcggctgta gcggtgatac gaaccttcgg cccagatgcc gagctgctgc cagaatgggt
       61 cgaacgcgcc atgcgtccac tggtcgaaca gatagttttc gtagtcggga tgatgccgca
      121 gcggcgaatg gcccggcttc cacggcatcc gggtgaacca ctcgcagatg tcctccgccg
      181 caagcgccgc atgcagcacc ggatcggcct gcgcttcggg cgacaccagc gcattcttga
      241 acgcccatgt cgcctgtttc agttcgaacg cgccggactg ccggatgccg gactgccatg
      301 aattgacgaa gccgccgcag tccagcacct gcgcaaccag ccccggcgga tccaggcatg
      361 ccagcgccgc ctgcgtgtgc gcggcatagg acagacccat cgtgcagata cgcccatcgc
      421 accacgtctg gtcgcgcagc cacgcacagg tatcgaaccc gtcctcgccg tccgacaggt
      481 atttgacgaa ccgtccctcc gacccatagc ggccgcgtgt atcctgaaac accaccgcat
      541 agccgtgcgc ggtgaaatac gccgccagtt ccgcgcgcga gaccggagtt ggttgcgccg
      601 cggtgatctc gctccggctg gcaatattcc ggccgtacgg cgtgcgctcc atgatcaccg
      661 gaaacggccc tgccccatcc ggcaaataca catcggtcgc gagacggatg ctgtcgcgca
      721 tcgcgaccat aggcgttgtt actggcatgc ctggatttgt cgcccaggac cgccgcgcca
      781 cgcaagctgg cctcaatcgg tgaccgtggg ctccagcgac ggcgaaacga gagcgggcac
      841 atggccgccg gcaagcacac gtatcgaggg aaagatcacg cgcaccgtgg tcccctgccc
      901 cggacggctc tctatcgtca gtttgccacc atgcatcgcc aacagcttgc gactgatcga
      961 cagtcccagt cccgttcccc cgtatgtccg gctgatcgac gcgtccgcct gggtgaacgg
     1021 ctgcccgagt gacggcagcg ccgctgaatc gatcccgata ccggtatccg ccaccgcgag
     1081 catcacgtcg ccgttggctg cccgcccgcc gcggatcgag accgtcccgc cactcggcgt
     1141 gaacttcacc gcgttggtca gcaggttcag cacgatctgc cgcaccgctc gagcatcggc
     1201 acgcaaaagg atctcccgct ccgcgatgct gcactccacg ccgacgcgct tggcctccgc
     1261 ctgcagcttc accatggcca ggcaagctcg cactgcgctg cccagatcga cccgatcctg
     1321 cgccagcgag tatcggccgg cctcgatccg cgccagttcc agcacctcgt tgatgacgtc
     1381 cagcaggtgc cgcccgctgg ccatgatgtc gccggcccat tccaggtaca cgtcgcccgc
     1441 tcgccccgat cgctgctgct tctgcatcag ctccgagaaa ccgatgatag cgttcaaggg
     1501 tgtccgcagc tcgtggctca tgttcgcgag gaactcggat ttcgcccggc tggccgcttc
     1561 ggcggcgtct ttcgagcgcc gcagctcctc ctccacctgc acctgtgccg tcacatcaca
     1621 cgcggtgccg cgatagccga tgaatgttcc cgcttcatcg tagatcggta aaccgctcgc
     1681 gctgatgtag cgcccgctgc catccgcagc gcgaaactgg aagcgaaaat cccggaacgg
     1741 ttttcgcgcg gcaagctcgg caatatgctc cggccacggc ggatcggtcc cgtcggatcc
     1801 ggccagttcc catcgggtca gaccgacata gctccgcctc ccgccagcgt tcagcagcgc
     1861 ggtctgggac agccagacaa accgcagatc cgcatcctgc tcccagaacc aatccgacga
     1921 tatctccgcg aagtcccgca cctggcgctc gctcgcccgt agccgctccg cgacacgcgc
     1981 cagttcccgc gcgtggctct cctgttggcg gaacagccag gaaatcgcga aaaacaaggc
     2041 ggtgaaaacc agcgcgacgc caactcggat gatcgccaca tcgaacgcat cacggtgcca
     2101 ggacgcaagc gcggcctgtg tcgtcacgct gacatcgaca ataagcggca ggtcgctcag
     2161 cgggcgtacc gcgacgatgg cacccggatc gccgaggtaa tcggcggcat cgtaagtgcc
     2221 gccaccttgc tccacctgct gataccaggc cgagcccgcc ggcatgcgtt ttccccgcca
     2281 gtcgcccaac tccgggtagc ccgcgatcac cacgccgtca tgattgagca gcgctacagc
     2341 ctggcccggc tgcatgctga ctttctggta gaagtcctcg agatatttcg catcgatcac
     2401 gccggccacc aggccgagca tcgtgccatc gggcgcatcg atccgccgca cgatcaacag
     2461 caacggcttg tggctggtgc gtccgagttt cggcccagac acatagacgc ccgccccggc
     2521 atggccacgc aggtgccggt agtaatcacg gtccgacaga tcgatctgct gcaccggcgt
     2581 atcgcgcgac cagttcagca gcattccttg cgcatcgacc aaggcgagcg cttccgcctg
     2641 cggcaggttc cgcaaacggc ctgccagcag ccgctgcacg ccttcgccag ccatctgctc
     2701 gcgaaattgc tcgggcgtcc gcacgtcgag ctgcgccgtg tgcgtctgga aatcgcgcag
     2761 aacgacatcg accgattgga tcgtgcgcgc ggtctgctca gccagggcta tgctcacgtt
     2821 ggccatatcg cggcgggcgc tggaaagcgc gctcgtccgc agatattcga ccgcggcata
     2881 acccgtgccg accagcatga tcatgacgag cgcccagatc acccacagcg cgccgcgccc
     2941 gccaatggag cggcgcgtac gcggcatacc accccgcgga gccatcagtg cgcgggcggc
     3001 catgctacag gctcagagaa tctgctgcga cgtcccggca cgtgctcgat cctggtttga
     3061 tcaggcaatg catcattcca gcttgcaaaa tcatttccgc tggaatccgg ataacgtctc
     3121 ggcctcaggt tcctccagct cgatgctgag ctcgcgcaaa gccgccgtgt gcgcatgctt
     3181 cgtgcgacct gcgcgcagat cgtcggcgct gagcagctcc accagttcgc ccccctgcat
     3241 cacgccgatc ctgtcgcaga gatgcgcgac cacagccagg ttgtggctga ccaggatgta
     3301 ggtcagcccg cgcgcctgct gcagatcgga caacaggttg agcacctcgg cctgcacgga
     3361 aacatcgagc gcgctggtcg gttcgtccag cagcagcacc tccggctccg ccatcagcgc
     3421 gcgcgcgatc gccacgcgct ggcgctgtcc gcccgacagc tggtggggat agcggaagcg
     3481 cacctcaggc gacagtgcca cctcgttcag cgcgcgcatg atgcggcgct cgatgttgtc
     3541 catccgatgc accagcagcg gctcgctgag cagccggtca accgtctggc gcgggtgcag
     3601 cgacccgtac ggatcctgga acaccatctg gacccgacgg tgaaaatcct tgccgcgacg
     3661 cggcgccatg atatcgccgt cgatcacgac ccgcccggtc cagtccgcgt tgaggccgga
     3721 cagggcgcgc agcaccgttg acttgcccga gccgctttcg cccaccaggc caaagctttc
     3781 gccgcgcgcc acctcgaatg aaacgtcctt caccgcctcg atctcggcga agcgcacatg
     3841 gagggcagcg acttcgatca ttcccccagc catgacgggt cgcgctgcat caccgcgagg
     3901 cgggtctgct tgtggctcag tgtcggcagg cattcgagca gtccgcgtgt atagggatgc
     3961 cgcgcactgg tcagctccga tgcccgcagc tcttccacga tccgccccgc atacatcacc
     4021 gcaacgcggt cgcagaaatg cgaaaccagc ggcaggtcgt ggctgatcag gatcaggccc
     4081 atgccgcggc gtgagacgag atcctcgatc aggcgcagga tctccgcctg caccgtggca
     4141 tcgagcgcgc tggtcggttc atcggcaatc agcagctcgg gatcgggcgc cagcatcatc
     4201 gcgatcatca cgcgctggcc catgccgccc gacaattcgt gcgggtaggc ctgcgcgaca
     4261 cgcgccggat cgcggatctg cacctgcgcc agaaggtcga tcgccgcctt ctccgccacc
     4321 ttccggctgc cgccccgatg cacgcgccac gcctcggcaa tctgcgcgcc ggcggtcatc
     4381 accgggttca gcgaatattt cgggtcctgc aggatcagcc cggcccgcgt gccgcgaatg
     4441 ctccgcagcg tcgcttcgtt ggcgcgcaat acatcgatgc cgtcgaaggt cagcgtatcg
     4501 gcagtggtga tcgcatagga cggcagcagc cgcaggatcg atcgcgcggt cagcgacttg
     4561 ccggaccccg actcgccgac aatgcccagc ttctcctgct gcagcgtcag cgaaacgttg
     4621 cggacggcat ccgacaaccc cgtgggcgtc gggaactgga tgcgcagatt ctggatctcg
     4681 acgagcatct agcgcaaacc atccaaatcc agtgtcatgg cgaggagcac agcgacgaag
     4741 caatctcgag ggaacgacgc gcgcaagacg cccccgcgct tcctccgtcg ccgcgctcct
     4801 cgccatgaca ggccgtaacc catcaccgtt gccgcgggtc cagcacgtcc cgcatcccgt
     4861 cccccagcag attgaacgcc agcgacgcca cgaagatcgc aacaccaggg atcgtcggca
     4921 cccaccactg ctcgagaatg aacgcgcgcg aagctgcgat catcgtgccc cattccggca
     4981 acggcggctg cgcccccatg cccaggaacc caagtcccgc cgcagtgagg atgatcccgc
     5041 tcatatccag cgtcacgcgc acgatcacgc ttgtcaggca cagcgggatg atgtggcgca
     5101 gaatgatccg ccccggtgac gcacccgtca gccgcaccgc cgcgatatag tcggtgtgcc
     5161 gcaccgtcag cgtatcggcg cgcgccagcc gcgcgtacgg cggccatgcc gtcagcgcta
     5221 tcgcgatgat cgcgctggtg atgccgggct tcagcgccgc gacaaatgcc agtgccagca
     5281 ccagccgagg aaacgccagg aacacatccg tcacacgcat caacgcgcga tccgccagcc
     5341 cgcccaggta gcccgcgatg ctgcctacag ccaggccgat cggtgccacc agcagcacga
     5401 tcgcgacgac catgccaagc gtgatccgcc cgccgtagat cacgcgggcg aacagatcgc
     5461 gacccaactc atccgttccg agcaggtgct cccaggacgg cggcgccaga cggttggcaa
     5521 ggtcctgcgc gctcgggtcc tgcggcgcga tcagcggcgc cgccagcgac accacgatca
     5581 tcagcacgat ggtgaccaga cccaccatcg ccagatgatt ggagcggaac tgcagccagc
     5641 cgcgataccg ccgcacccac gcggcgtggg cacgcgaggc tggcgcctcg ctcaacagcc
     5701 agtctcggga caaggcgatc attgcactcg cgggtccagc aagcgatatg ccatatcgga
     5761 cagcaggttc agcaccacat acaccatgcc gatcaccagc gtggcaccca gcacggcgtt
     5821 catgtccgcg ttcagcagcg ataccgtcag gtaaagcccg atacccggcc aggaaaacac
     5881 ggtctctgtt atgaccgccc cctcgagcag actggcataa gccagcgcca gcacggtcac
     5941 cagccggacc gccatgttgc cgaatgcgtg cttccacagg atgcgcgccg gcgacagccc
     6001 ctttgcccgc gccgtgatga tgtactcgcc cgacagcgcc tcgatcatga aggcgcgtgt
     6061 catgcgcgtg atgtagccca tcgagaaata cgccagcacc aacgccggct gcgccagatg
     6121 cgccagggca tcccagaacg aatcccagtc attcgcgagc gccgcatcga tcaccagcat
     6181 gccggtgacg cgtggcacca tgccctcgaa ccccacatcg ttctggcccg tgcctggtgc
     6241 gatccccagc ttcacataga acaccagcag acagatcagc cccagcacga acaccggcac
     6301 cgactgtccc gccagactga tcacccgcac cacatgatcg aaccgcgagc cttgccgcgc
     6361 cgccgcgaac acgcccaacg gaacgccgac gaggatcgcg atgatgatcg ccgcagtcgc
     6421 cagttccagc gtggcgggaa agaaccgcgc aatgtcggcg gtgaccggac gcgtggtcat
     6481 caccgaggtg cccaggtcgc cgtgcgccag attgctcaga tagatgccga actgcacgta
     6541 aagcgggcga tccagtccca gttccgtacg cacacgcgcg atcacatcgg gcggcgcatg
     6601 atcgccaacg atggcgatga ctggatcgat cggcatcacg cggccgatca ggaacgtcac
     6661 cagcagcaaa ccgaacagcg tgaccggcac gctggcgatt gccgacaccg ctggatgcag
     6721 tcgcgtcgga aatcgccagc gtgttgtctg ggtggtggcg ctcacgcctt ggtaatcgcc
     6781 gcgtagcgtg tgtagtccgg cagcacaccg atctggatgc ctgcgacgcc cttgcgcata
     6841 acgtccacct cggcgttctg caacagcatg acgaacggcg cacgctccat gaacgcacgc
     6901 tgcatcttgg cgtacatcgc catgcgcttc gcggtgtcga gttccttcgc cgcttcctcc
     6961 acgccggcgg tcagctcctt gtcgaagaaa tggcagcgcc aggcgaggat cttcagcttg
     7021 ctgttgtcac cgtcatccgg gttggccgcg aacgcctgca tgttggagtt cgcatccagg
     7081 taatccggga accaggtctg cagcaccagc tgatgctgcc gcgcgcgcat cttgctgatc
     7141 acctgcttat gctcgcccgc cagcaactgc gccttgattc ccaccgccgc cagatccgcc
     7201 tggatcgcct gcgcgatgac cgagtacggc gcgttggaga aatggtccaa cgtgaccgag
     7261 aagccgtccg gatagccagc ctgcgccagc agcgccttcg ccttcgccac atccttcttg
     7321 aacggcgtat cgctgatcgc gcctggcaca ccctttggca gcaccgattg ccacacgttc
     7381 cagacattcg gcgtgatgtt cttggcgatc gcctcgtagt cgatcgccca cttcagcgcc
     7441 tgcaacacct gcaccttctg gaacatgggc tgccccatgt tggccgccag atacatcgac
     7501 gtaagctgcg gctcacgcgc gacgctgaat tccgactggc cgaccacgga cttgagctga
     7561 tcc
//
LOCUS       DATGEM010000557         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1520295, whole genome shotgun sequence.
ACCESSION   DATGEM010000557 DATGEM010000000
VERSION     DATGEM010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520295"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..421
                     /locus_tag="VKQ27_17130"
     CDS             218..421
                     /locus_tag="VKQ27_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90708.1"
                     /translation="MQDWKEADVHAVLVVRYGKLIYENYFTGTDEPLGGQARSVTFDV
                     AAKHDLRSIEKRIFSLLVGIAIG"
     gene            546..1148
                     /locus_tag="VKQ27_17135"
     CDS             546..1148
                     /locus_tag="VKQ27_17135"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLY90709.1"
                     /translation="MFDPHNNETNMHFSSDPARYALDQPVEFPPGTAYRDDSGSVVVL
                     GGLLEKATGQKLDDFDRTALFSPLGITDFGWVHLRAGVTSPFGLRMRPRDLAKIGQLV
                     LNQGAWQTDCASGLGSGAITPLIHGQQLYFCGYLFWLGRSLLRGQEVDWAAGVGLGGQ
                     RPFFVPALDLGVVVHPGCTAARCRAPRHWSFWTASYCRAV"
     gene            complement(1168..2007)
                     /gene="folD"
                     /locus_tag="VKQ27_17140"
     CDS             complement(1168..2007)
                     /gene="folD"
                     /locus_tag="VKQ27_17140"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HLY90710.1"
                     /translation="MTARIIDGTAIAASLRTRLADKVAALGYQPGLAVVLVGEDPASA
                     VYVRTKDKAANAAGIAAQTIRLPSTSTTSDVLAKIAQLNADEAIDGILVQLPLPPHID
                     TQTVLEAVDPAKDVDGFHPLNVGRLASGHPTLVPCTPLGIMRLLHEAGIPLEGERALI
                     AGRSAIVGRPIVSLLLAANATVTVAHSRTRDLPAECRRASVVVAAVGKPELIRGNWIA
                     PGATVIDVGINRLPSGKLVGDVAFAEATQVAGAITPVPGGVGPMTIACLLENTVTAAV
                     RRR"
     gene            complement(2004..>2176)
                     /locus_tag="VKQ27_17145"
     CDS             complement(2004..>2176)
                     /locus_tag="VKQ27_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HLY90711.1"
                     /translation="EPPEDGRANRAVCAAIADLLDVAQSAVTVTVGATSRDKTLRITG
                     DPTALGAKLAAL"
BASE COUNT          359 a          626 c          758 g          433 t
ORIGIN      
        1 ctgtccttcg cggcctgaga cggggtgtga tcttaccgcg cggcgctttc tacgcgggcc
       61 gccaatgcgg atcgtggttg cggtcgtgcc tcgctggtcg ttcgcggaac tcggcatccg
      121 attatggcac tccaaaggag atggaggacg gatgacaaat cgaatccgcc gcgtccgtcg
      181 ggctcgaatt ggcgacgctt gccgccttgg acgacgtttg caggactgga aggaggcgga
      241 tgtccacgct gtgctggtcg tgcggtacgg caaactgatc tacgagaact acttcactgg
      301 cactgacgaa ccgctgggcg gccaggcccg cagcgtaacg ttcgacgttg ccgcgaagca
      361 cgacctgcgc tcgatcgaga agagaatttt ctctctgctg gtgggaattg ccatcggata
      421 gggcaggatc tctgggattg atcagccggt gttgcctata ctcgccgctc ggctgataaa
      481 gatcgcgtca cactgcgtca cctgctgacc ctgtcgctgg gtctcgcgtg gaatgaggac
      541 ctgctatatt cgatcctcac aacaacgaaa cgaacatgca cttttcatcc gacccggcgc
      601 gctatgcatt ggatcagccg gtagaattcc ctcccggaac ggcatatcgc gacgactcgg
      661 gttcggtagt cgtcctcggt ggcttgctcg agaaagctac tggccagaag ttggatgact
      721 ttgaccgtac cgcgttgttt tcgccgttgg gcatcactga cttcgggtgg gtacaccttc
      781 gggccggcgt gacatcgccc tttgggcttc gcatgcggcc gcgggacctg gcgaagatcg
      841 gacaactggt actgaaccaa ggggcatggc aaacagattg cgccagcgga ttgggttcag
      901 gcgctatcac gccactgata catgggcagc agctgtattt ttgtggctac ctgttctggc
      961 ttggccggtc gttgctgcgc ggacaggagg tggattgggc ggctggcgtt ggcctgggtg
     1021 ggcagcggcc gtttttcgtc ccggcgttgg atctgggcgt cgtggtgcat ccgggctgta
     1081 cagcagcccg ctgccgggca ccgcgccatt ggtcattctg gaccgcttcg tattgccggg
     1141 cggtttagcc tcgataagcg cgcggggtta ccggcgccgc acggctgccg tcaccgtgtt
     1201 ctccagcagg caggcgatcg tcattgggcc gacaccgccg gggacggggg tgatcgcgcc
     1261 ggcgacctgg gtggcttcgg cgaatgccac atcgcccacc agcttgccgc tgggcagtcg
     1321 gttgatgccg acatcaatga ccgtcgcgcc tggggcgatc cagttgccgc ggatcaactc
     1381 gggcttgcca actgcggcca cgacgacgga tgcgcggcgg cattcggcgg ggagatcgcg
     1441 ggtgcgagaa tgcgcgacag tgactgtggc attggcggcc agcagcagcg agacaatcgg
     1501 gcggccgacg atggcggagc ggccggcgat cagcgcgcgc tcgccttcca gcgggatgcc
     1561 ggcttcgtgc aacagtctca tgatgcccag cggcgtgcag ggaaccaggg ttgggtggcc
     1621 gctcgcgaga cggccgacgt tcaatgggtg gaacccgtcc acatccttgg cggggtccac
     1681 ggcctcgagc acggtctgcg tatcgatatg aggcggcaag ggaagctgca ccaggattcc
     1741 atcaatggcc tcgtcggcat tcagttgcgc gatctttgca agaacgtctg atgtcgtact
     1801 cgtggacggc agacggatgg tctgcgcagc gatgccggcg gcgttggcgg ccttgtcctt
     1861 ggtgcggacg tagacagcgc tggctggatc ttcacctacc agcacaactg ccagtccggg
     1921 ttggtatcca agggctgcga ccttatcagc aagccgggtg cggagggatg cggcgatcgc
     1981 ggtaccgtcg atgatgcggg cggtcataac gcggccaact tggcgccgag tgcagtagga
     2041 tcgccggtga tgcgcaaggt cttgtcgcga ctggtggcgc caacggtgac ggtgacggcg
     2101 gattgcgcga cgtccagcag gtcggcgatg gcggcgcaga ctgctcggtt ggcgcggccg
     2161 tcttccggcg gctcgg
//
LOCUS       DATGEM010000558         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_149389, whole genome shotgun sequence.
ACCESSION   DATGEM010000558 DATGEM010000000
VERSION     DATGEM010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149389"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VKQ27_17150"
     CDS             <1..607
                     /locus_tag="VKQ27_17150"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLY90712.1"
                     /translation="NKDPNRYLMSRSMAPALVTSEDILEYDLDSTPIDQRGRAVYLER
                     FIHGEIYKARPEVRAIVHSHSPSVIPYADTKMQLRPMNHIAGFLGGGVPVFEIRDIGG
                     PATNMLISNPKLGHALAVTLGQHPVALMRGHGSVAAAQSVRHVVFRAVYTEVNARVES
                     EALRLGEVTFLNDAEAEAAMKTNDALVDRPWDLWKRKAMGK"
     gene            complement(611..1978)
                     /locus_tag="VKQ27_17155"
     CDS             complement(611..1978)
                     /locus_tag="VKQ27_17155"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLY90713.1"
                     /translation="MTVASEPLRQFLQAARSAGIRVSAAEGIDAARAVQVVGYADRTV
                     LKDTLGLVLAKTPDEKHLFDECFDLYFRRDQLAQDEEEPATDPQAPITPSGFGGEGTG
                     GGGGRSLSQMLLDDDRAAMAAAMEEAARESGIQNIRFFTQKNLYARRILDRMGLRQVE
                     RDMEAKRKDGDQATADRLENRVEALRDQARDLVERNLLLFARGETEKFREELLKSARL
                     SNLERRDLDRMRILVRAMAKRLAARYAKTRRRRLRGQLDVRRTLRRNMGWGGVPFVTV
                     WKQKRIEKPRVMVLCDVSGSVAAMAQFLLMFLYALNEALKDIRSFAFASSLMEVSDIL
                     ESEPIEAAIARIMQAIGFGSSNYGNSLADFEDGWERYVTNKTTIIILGDARGNRTDPR
                     TDIMQRLSQRAKRIIWLNPEFRSAWGTGDSDMFRYSPYCNVVTVCNTLRHLERVVTDL
                     LKDAA"
     gene            complement(1975..>2620)
                     /locus_tag="VKQ27_17160"
     CDS             complement(1975..>2620)
                     /locus_tag="VKQ27_17160"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLY90714.1"
                     /translation="DVIGASETLPAALTKLHAFGDIFFSEQFLEPRPLLRALQQPGGA
                     VLLIDEIDKSDQEFEAFLLEILSDYQVTIPEIGSVHAVQTPIVMLTSNSTRDLGDALK
                     RRCLHLHIGFPDAKLEGRIIESRVPGIETSLRRELVAFVQQLRTLDLKKVPSISETID
                     WARVLVLLHAAALNTELVHDTLNVLLKFEADIEVANKQLATLTQKAHQEAGLPG"
BASE COUNT          447 a          792 c          874 g          507 t
ORIGIN      
        1 caacaaggat ccaaatcgct acctgatgtc gcgcagcatg gcgcccgcgc tggtgacgtc
       61 ggaggacatc ctcgaatacg acctcgacag tacgccaatc gatcagcgtg gccgcgcagt
      121 gtacctcgaa cggttcattc atggcgaaat ctataaggca cggcccgagg tgcgcgcgat
      181 cgtgcacagc cattcgccgt ctgttatccc atatgctgat acgaagatgc agctccggcc
      241 gatgaaccat atcgcggggt tcctgggcgg cggtgtgccg gtgttcgaga tccgtgatat
      301 cggcggtcct gccaccaata tgctgatcag caatccgaag ctggggcatg cgctggcggt
      361 aacgttggga cagcatccgg tcgcgctgat gcgtggccat ggctcggtgg ctgcggcgca
      421 gtcggtgcgc catgtcgtgt tccgtgctgt gtatacggag gtcaatgcgc gggtggagag
      481 cgaggcgcta cggctgggtg aggtgacatt cctgaacgat gcggaggccg aggcggcgat
      541 gaagaccaac gatgcgctgg ttgatcgacc gtgggaccta tggaaacgca aggcgatggg
      601 gaagtaggcg ctaggcggcg tccttcagca ggtccgtcac cacgcgctcg aggtggcgca
      661 gcgtgttgca gacggtgacc acgttgcagt agggcgagta gcggaacatg tcggaatcgc
      721 cggtgcccca ggcggagcgg aactccgggt tcagccagat gatccgcttc gcccgctgcg
      781 acaggcgctg catgatgtcg gtgcgcggat cggtgcggtt gccacgcgca tcgcccagga
      841 tgatgattgt ggtcttgttg gtaacgtaac gctcccagcc atcttcgaaa tccgccagcg
      901 agttgccgta gttggaggag ccgaagccga tcgcctgcat gatcctggcg attgctgcct
      961 cgatcggttc ggattcgagg atgtcgctga cttccatcag gctgctggcg aaagcaaatg
     1021 aacggatgtc cttcagtgcc tcattcagcg cgtagaggaa cattagcagg aattgcgcca
     1081 ttgcggcgac cgagccggat acatcgcaca gcaccatcac gcgtggcttt tcgatacgct
     1141 tctgcttcca cacggtgacg aagggcacgc cgccccagcc catgttccgg cgcagcgtgc
     1201 ggcgcacgtc gagttggccg cgcaacctgc ggcgacgggt cttcgcgtaa cgggcggcca
     1261 ggcgcttcgc cattgcgcgc accaggatac gcatccggtc cagatcgcgg cgctcgaggt
     1321 tgctgaggcg cgcggacttc agcagttcct cgcggaattt ttctgtctcg cctcgcgcga
     1381 acagcagcag gttgcgttcc accagatcgc gggcctggtc gcggagggct tcaacgcggt
     1441 tctccagccg atcggccgtg gcctgatcgc cgtccttgcg cttcgcctcc atgtcgcgtt
     1501 cgacctgacg caggcccata cgatcgagga tacggcgggc gtacaggttc ttctgagtga
     1561 agaagcggat gttctggatg cctgattcgc gcgcggcctc ttccatcgcg gcggccatcg
     1621 cggcgcgatc gtcatcgagc agcatctgcg acagagaacg gccaccgccg ccacccgtgc
     1681 cttcgccacc aaatccactc ggcgtgatcg gcgcttgcgg atccgtcgcg ggctcttctt
     1741 cgtcctgtgc gagctggtct cgccggaagt agaggtcgaa gcactcgtcg aacaggtgct
     1801 tttcgtccgg cgtctttgcc agcaccagcc ccagagtatc cttcagcacc gtgcggtcgg
     1861 cgtaccccac cacctggacg gcgcgggcgg cgtcgatgcc ctcggcggcg gacacgcgga
     1921 tgccggcgct gcgtgccgcc tgcaggaact ggcgcagcgg ttcgctggcg actgtcatcc
     1981 cggcaggcca gcctcctgat gtgccttctg cgtcagcgtc gcgagctgct tgttcgccac
     2041 ttcgatgtcc gcttcgaatt tcagcagcac gttcagcgtg tcgtgcacca gttcggtgtt
     2101 cagcgccgcg gcgtgcagca acaccagcac gcgcgcccag tcgatcgtct cgctgatcga
     2161 gggtaccttc ttcaggtcga gcgtgcgcaa ctgctgtaca aaggccacga gctcgcggcg
     2221 cagggatgtc tcgatgccgg ggacgcgtga ttcgatgatg cggccttcga gtttcgcgtc
     2281 ggggaagccg atgtgaagat gcaggcagcg gcgtttaagc gcatcgccga ggtcgcgggt
     2341 actgttggag gtcagcatca cgatcggtgt ctgcaccgca tggacgctgc cgatttcagg
     2401 gatcgtcacc tggtagtcgg agaggatctc cagcaggaac gcctcgaatt cctggtcaga
     2461 cttgtcgatc tcgtcgatca gcagcacggc gccgccgggc tgttgcagcg cgcggagcag
     2521 cgggcgcggc tcgaggaatt gctcgctgaa gaagatgtca ccgaaggcgt gcagcttggt
     2581 cagggcggcg ggcagggttt ccgacgcgcc gatcacgtcg
//
LOCUS       DATGEM010000559         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_493883, whole genome shotgun sequence.
ACCESSION   DATGEM010000559 DATGEM010000000
VERSION     DATGEM010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493883"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..954)
                     /locus_tag="VKQ27_17165"
     CDS             complement(88..954)
                     /locus_tag="VKQ27_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HLY90715.1"
                     /translation="MKGYNFAYLDEQTKRMIRRALLKAVAIPGHQVPFGSREMPLPYG
                     WGTGGIQVTASILGSDDVLKVIDQGADDTTNAVSIRRFFARTAGVRTTTHTGDATVIQ
                     TRHRIPEKQLRDDQIIVYQVPQPEPLFRLEPSRIESRRMHSLADYGLMHVKLYEDIAR
                     FGQISISYDYPVMVNQRYLMSPSPIPAFDNPKMHLMPALQLFGAGREKRIYAIPPWTD
                     VRSLDFADHPFRRINAARPCGLCGATDAYLDEVVVDDRGGRLFVCSDTDHCAERRAAG
                     HVGSEGMKEAAD"
     gene            complement(1013..2104)
                     /locus_tag="VKQ27_17170"
     CDS             complement(1013..2104)
                     /locus_tag="VKQ27_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HLY90716.1"
                     /translation="MAYVTVKGGEQAIDNAHAWLAEERRGDRSVPDVTLAQIEEQLGR
                     AVDRAMGEGSLHDRELAALAVKQAQGDLIEAAFLLRAYRTTLPRFAHSEPVDTAGMQI
                     QRRISAVFKDLPGGQILGPTYDYTHRLLDFTLVANNEATHAPDGADELAAMPRVPDIL
                     GQEGLIEPDAQGESEPGDLTREPLAFPAARDVRLQALARGDEGFLLALGYSTQRGYGG
                     THPFAGEIRMGEVSVEIEPPELGFAIDIGDITVTECQMVNQFKGSKTTPPQFTRGYGL
                     VFGFSERKAMAMALVDRAMRAEELGEDVSAPAQNVEFVLYHSDNIEATGFVEHLKLPH
                     YVDFQSELQNVRRMRAEMERATERAEAAE"
     gene            complement(2108..2689)
                     /gene="phnH"
                     /locus_tag="VKQ27_17175"
     CDS             complement(2108..2689)
                     /gene="phnH"
                     /locus_tag="VKQ27_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HLY90717.1"
                     /translation="MSATITLPGFADPVGDAQATFRAALDAMARPGTLHQAVANLTPP
                     APLDRATAAVLLALVDNETPLWIDQAATDAGDWLAFHCGATIIERQADAEFALALALP
                     DLTAFSSGTDEAPESSVTMIVQLAALGHGARYRLTGPGLREPAMLAASGLPANFPAIW
                     QQNRALFPRGVDIVLCADTTLTALPRSVTIEEA"
     gene            complement(2686..3153)
                     /gene="phnG"
                     /locus_tag="VKQ27_17180"
     CDS             complement(2686..3153)
                     /gene="phnG"
                     /locus_tag="VKQ27_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HLY90718.1"
                     /translation="MLAATSPEQPHAARRRWMSVLARAKAADISRRLGACTELPRYRM
                     LRGPEAGLVMVRGRIGGGGSAFNLGEMTATRCTIRTDDGLVGHAYVAGRDERLAEVAA
                     LADALLQDPRRQPDILHDVIEPLAADQQAKRDAVAAKAAATRVQFFAMRNMRT"
     gene            3259..>3914
                     /gene="phnF"
                     /locus_tag="VKQ27_17185"
     CDS             3259..>3914
                     /gene="phnF"
                     /locus_tag="VKQ27_17185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HLY90719.1"
                     /translation="MTAPEPVLRQEGVSLWRQIANRLQHDIGAGIYVPGGRLPTEAEL
                     SQHFRVNRHTVRRALEELSRGGLVRVEQGRGSFVAEDVLEYAVEPRTRFSEWIRRHNM
                     EPSGRVLQLKETAADSHVASALGIRSGSRVILFERLGFADERPVSLSQHYFPAARLRG
                     LLDALRNSLTITEALKAVGVNDYLRQNTRVTARLPNAEEAEMLRMPRNRPLLITENIN
                     V"
BASE COUNT          661 a         1246 c         1264 g          743 t
ORIGIN      
        1 gccacagatc gacatctacc tgcgcgagag catgcagcgc gccgtaggac agggacaggc
       61 cccgcgcaga aagcagcgtg tcttcggtca atctgccgcc tccttcatgc cttcactgcc
      121 gacgtgcccg gccgcgcggc gttccgcgca gtgatctgta tcggagcaga caaacagccg
      181 cccaccgcga tcgtcgacaa ccacttcgtc cagataggca tccgtcgcgc cgcacaagcc
      241 gcatggtcgc gccgcgttga tccgccggaa cgggtgatca gcgaagtcca gcgaccggac
      301 atccgtccag ggtggaattg cgtagatacg cttctcgcgg ccagcaccga acaactgcaa
      361 cgctggcatc agatgcatct tgggattgtc gaaggctgga attggacttg gcgacataag
      421 gtaacgttgg ttcaccatca ccggataatc gtagctgatc gagatctgtc cgaaccgggc
      481 aatatcctcg tacagcttca catgcatcag tccgtagtcc gcgagcgaat gcatgcggcg
      541 gctttcgatg cgcgatggtt ccagccggaa cagtggctcc ggctgcggta cctggtagac
      601 gatgatctgg tcgtcgcgca gttgcttttc cgggatacga tgccgtgtct ggatgacagt
      661 ggcgtcgccg gtgtgggtcg tggtgcgcac gcccgcggtg cgagcgaaga agcgccggat
      721 cgacaccgcg ttggtcgtgt cgtccgcgcc ctggtcaatc accttgagta cgtcatcact
      781 gcccaggatg gaggcggtga cctggattcc gccggtgccc cagccataag gtagcggcat
      841 ctcgcgggac ccgaagggca cctggtggcc tgggatcgcc accgccttca acaatgcccg
      901 gcggatcatc cgcttcgtct gttcgtcgag atacgcgaaa ttgtagccct tcatgcgtgg
      961 tcccgggcgg atgcccggcc ttctgccgtc acggccggac ctgctccggc catcattccg
     1021 cggcctcggc gcgttcagtg gcgcgctcca tttccgcccg catgcggcgt acgttctgca
     1081 gttcgctctg gaagtccacg taatgcggca gcttcaggtg ttcgacgaag cccgtcgcct
     1141 cgatgttgtc cgagtggtac agcacgaact cgacgttctg ggccggtgcc gacacatctt
     1201 caccgagttc ttcggcgcgc atggcacgat caacaagcgc catggccatt gccttgcgct
     1261 cggagaagcc gaacaccagg ccgtagccac gggtgaactg tggcggcgtt gtctttgatc
     1321 ccttgaattg gttgaccatc tggcactcgg tcacggtgat atcgccgatg tcgatggcga
     1381 aaccgagttc cggcggctcg atttccaccg agacctcgcc cattcggatt tccccggcga
     1441 acggatgcgt gccgccatag cctcgctggg tcgaatagcc gagcgccagc aggaagcctt
     1501 catcgccgcg cgccaacgct tgtagcctga cgtcgcgggc cgcggggaac gccaggggtt
     1561 cgcgggtcag atcgcctggc tcgctctcgc cttgggcgtc gggctcgatc aacccctcct
     1621 ggccaaggat atccggcacg cgcggcattg cggccagctc gtctgcgcca tcaggagcat
     1681 gggtcgcttc attgttagcg acgagagtga agtccagcag tcgatgcgta tagtcatatg
     1741 taggtccgag gatctgtccg ccgggcaggt ccttgaagac tgcggagatg cgtcgctgga
     1801 tctgcattcc agccgtatcc acaggttcgg agtgcgcaaa tcgaggcaag gttgtgcggt
     1861 aggcgcggag caggaacgcc gcctcgatga gatcaccctg cgcctgcttc acggcaagcg
     1921 cggcaagttc cctgtcgtgc agcgagcctt cccccatcgc gcggtccacc gcccggccga
     1981 gctgttcctc gatctgcgcc agcgtcacat ccggcaccga gcggtcgccg cgccgctcct
     2041 ccgccagcca ggcgtgcgcg ttgtcgatcg cctgctcgcc gcctttgact gtgacatagg
     2101 ccatggctca ggcctcctcg atcgtgacgc tgcgcggcaa tgcggtcagt gtcgtgtcgg
     2161 cgcacaacac gatgtccacg ccgcgcggaa acagcgcgcg gttctgctgc cagatcgccg
     2221 ggaagtttgc gggcagtccg cttgccgcaa gcatcgcagg ttcgcgcaat cctggccccg
     2281 tgaggcggta ccgtgcgccg tggcccaatg ccgcaagctg cacgatcatc gtcaccgaac
     2341 tttcaggtgc ttcgtcggtg ccggaggaaa atgcggtcag atcgggaagt gccagcgcca
     2401 gcgcgaattc ggcatcggcc tgccgctcga tgatcgtggc cccgcaatgg aaggccagcc
     2461 aatcgccagc atcggttgcg gcctggtcga tccagagcgg tgtctcgtta tcgaccagtg
     2521 ccaacaatac cgcagcggtc gcacggtcaa gcggcgccgg cggggtcagg ttggcgacgg
     2581 cctgatggag cgtgccaggg cgagccatgg cgtccagcgc agctcggaag gttgcctgcg
     2641 cgtcgccgac cggatcggcg aagccaggca gtgtgatggt cgcactcatg tccgcatatt
     2701 ccgcatggca aagaactgta cgcgggtggc cgccgccttt gctgccacgg catcgcgctt
     2761 cgcctgctga tcggctgcca gcggctcgat gacatcgtgc aggatatcgg gctgacgccg
     2821 tggatcctgg agcaatgcat cggcgagcgc cgcgacttca gcgagtcgct cgtcgcgtcc
     2881 cgcgacatag gcatgcccga caagcccgtc gtccgtgcgt attgtgcacc gggtggcggt
     2941 catctcgccc aggttgaatg cactgccgcc tccgccgatc cggccacgca ccatgaccag
     3001 ccctgcctct ggcccacgca gcatgcgata cctgggcagt tcggtgcacg cgccgagtcg
     3061 gcggcttatg tctgcggctt tcgcccgggc aagcaccgac atccagcggc gtcgggcggc
     3121 gtgcggttgt tccggcgagg tggcggcgag cattggcgag agcctgcgct tgttcggatg
     3181 tctagacagc tagaccctga ctcggctatc gtcaagcgag gagttgcatg gatggtcttt
     3241 cctgtggatg acattgcaat gacggcaccc gagccagtgc tccggcagga gggggtttcc
     3301 ctgtggcgac agatcgccaa ccggctgcag cacgatattg gcgctggtat ctacgtgccc
     3361 ggtggtcgat tgccgacgga agcagagttg tcgcagcact tccgtgtgaa ccgccatacc
     3421 gtcaggcgcg cattggaaga gctgtcacgc ggtggcctcg tgcgtgtgga gcagggccgc
     3481 ggctcgttcg tcgccgagga cgtgctggaa tacgcggtcg agccgcgcac gcgcttctcg
     3541 gagtggattc ggcgccacaa catggagcct tcgggccggg tgctgcagct caaggagacg
     3601 gcggccgatt cgcatgtcgc aagtgcactt ggcatccgct cgggcagccg cgtcatcctg
     3661 ttcgaacgac ttggtttcgc ggatgagcgg ccggtgagcc tgtcgcagca ttactttccc
     3721 gcggcccggc tgcgcggatt gctggatgcg ttgcgcaact ccctgaccat caccgaggct
     3781 ttgaaggcgg ttggcgtcaa tgactatctg cggcagaaca ctcgcgtcac tgcacgcctg
     3841 cctaatgccg aggaagccga gatgctccgc atgccacgca accggccgct gctgatcact
     3901 gagaacatca atgt
//
LOCUS       DATGEM010000560         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_377594, whole genome shotgun sequence.
ACCESSION   DATGEM010000560 DATGEM010000000
VERSION     DATGEM010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377594"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VKQ27_17190"
     CDS             complement(<1..1396)
                     /locus_tag="VKQ27_17190"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HLY90720.1"
                     /translation="MSKWVYSFGAGHNEGRADMRNLLGGKGANLAEMASIGLPVPPGF
                     TLTTEVCTAFYQNQRQYPNDLGKQVSQAMARLEKAQGLKFGDKLKPLLVSVRSGARVS
                     MPGMMDTVLNLGLNDITVEGLGEEADDPRFAWDSYRRFIQMYGSVVLGVDHHRFEEII
                     EHAKLDAGVTEDTQLTAQDWHRVAQSYKDMIEGETGKAFPQDPEEQLWGAIGAVFGSW
                     MNHRAITYRKLHDIPAEWGTAVNVQAMVFGNMGNDCATGVCFTRDPSTGENAFYGEYL
                     VNAQGEDVVAGIRTPQPVSKARAKPDETPLEVAMPGPYKELLKVRETLEQHYKDMQDL
                     EFTIQKSKLYMLQTRNGKRTAAASLKVAVDMANEGLIDETEAVRRVNPSALDQLLHPT
                     LDPKAPRTLLAKGLPASPGAASGAVVFTADEAESRAQKGEDIILVRIETSPEDIHGMH
                     AARGILTTRGGMTSH"
     gene            complement(1406..3466)
                     /gene="glyS"
                     /locus_tag="VKQ27_17195"
     CDS             complement(1406..3466)
                     /gene="glyS"
                     /locus_tag="VKQ27_17195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HLY90721.1"
                     /translation="MPELFLELFSEEIPARMQSRAAEDLARQASEALAALSPSGIRTY
                     HGPRRIAFAGEVAPQVAAASSAERGPRTSAPEQALTGFLRKHNAGREQLRQDGDYWVL
                     EKSSAAVPAASLIAAAIPPLLRRFPWPKSMRWGGTSNFVWVRPLRRIVCLLDGQVVPF
                     NLRDGADDGHGLASGYLTEGHRFLAPGAVTVSSAADWSAKLRERRVLVDADDRKRLIA
                     DGIAALAAERQIALVDDPALLDEVAGLVEWPLPLLGRIDDAYMDLPAEVRQVSMRVNQ
                     RYFAFRNPDGSAAPWFGFVANIEPADGGAASTAGNERVLRARFADARHFWDMDRRARL
                     ESRVPLLDNVSFHAKLGSQGERVRRLERLAEEIAPLVGAGPALARQAARLAKADLTTG
                     MVGEFPELQGVMGRYYALHDGEDPLVADAIRDHYAPKGPNDAVPTSDVSIAVALADKL
                     DLLVGFFAIGERPTGGGDPYALRRAALGIIRIVRENGLRLNLLPLITLAGEAWHHRGV
                     HPAPPQEVLDFLAERLRVQLRTEGARYDVLAAIFAAHPDDDIMRLVARTDALAQFLRS
                     ADGSDLLLAYRRAANISRIEQRKGGPSDDAVDAQLIRINEEEALARQVDLASSRMREH
                     LEADQFVDALSALATLREPVDAFFEKATVNAPEPELRLNRLRLLNRLCTTMRRVADFS
                     RIEG"
     gene            complement(3739..>3906)
                     /locus_tag="VKQ27_17200"
     CDS             complement(3739..>3906)
                     /locus_tag="VKQ27_17200"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLY90722.1"
                     /translation="AEACQLAAELAELTGHSLRLAVVTALREERDRALSRRRRHERIM
                     AITREIALSAA"
BASE COUNT          657 a         1329 c         1236 g          684 t
ORIGIN      
        1 cgtggctggt catgccgcct cgcgtggtga ggatgccgcg ggccgcgtgc atgccatgga
       61 tgtcctcagg actggtctcg atgcgcacga ggatgatgtc ttcgcctttc tgcgcccggc
      121 tctccgcctc atccgcggtg aacaccaccg cgccagacgc ggcaccgggg ctggccggca
      181 accccttcgc cagcaacgtg cggggtgcct tcggatcaag cgtcggatgc agcagctggt
      241 ccagtgccga cggattgacc cgccgaaccg cctcggtctc gtcgatcagc ccctcattcg
      301 ccatgtccac ggcgaccttc agagacgctg ccgccgtgcg ctttccgttg cgggtctgca
      361 gcatgtacag cttgctcttc tggatggtga actccaggtc ctgcatgtcc ttgtagtgct
      421 gctcgagagt ctcgcgaacc ttcagcagct ccttgtaggg acccggcatc gccacctcga
      481 gcggggtctc gtccggcttc gcccgcgcct tcgacaccgg ctgaggcgtg cggatgcccg
      541 ccaccacgtc ctcaccctgc gcgttcacca aatactcgcc gtagaacgca ttttcgcccg
      601 tcgacggatc gcgggtgaag cacacgccag tcgcgcaatc gttgcccatg ttgccaaaca
      661 ccatggcctg gacgttgacc gcggtgcccc actcggcggg gatgtcatgc agcttccggt
      721 aggtgatggc gcggtggttc atccacgagc cgaataccgc gccgatggct ccccacagct
      781 gctcttccgg gtcttgcggg aacgccttgc cggtctcgcc ctcgatcatg tccttgtagc
      841 tctgcgccac ccggtgccag tcctgcgcgg taagctgggt gtcctcggtt acgccggcgt
      901 cgagcttggc gtgctcgatg atctcctcga accggtgatg gtcgacgccg agcacgaccg
      961 agccgtacat ctggatgaac cgccggtagg agtcccaggc gaagcgcggg tcatcggcct
     1021 cctcgccgag tccttccacg gtgatgtcgt tcagcccgag gttcagcacg gtatccatca
     1081 tgcccggcat ggacacccgc gcgcccgaac gcaccgagac cagcagcggc ttaagtttgt
     1141 cgccgaactt cagcccttgc gccttctcca gccgggccat cgcctgcgac acctgcttgc
     1201 ccaggtcgtt cgggtactgc cgctggttct gatagaacgc ggtgcacacc tcggtcgtga
     1261 gggtgaagcc gggtggcacc ggcaggccga tgctcgccat ttcggccagg ttcgcgccct
     1321 tgccgcccaa caggttgcgc atgtcagcac gaccctcgtt gtgaccggct ccgaagctgt
     1381 acacccattt gctcatagcg aactcctagc cctcgatacg tgaaaagtcg gctacccggc
     1441 gcatcgttgt gcagagacgg ttaaggagac gcaaacgatt gaggcgcaat tctggctcgg
     1501 gagcattgac ggtcgccttc tcgaagaagg catcaaccgg ctcccgcagc gtagccagtg
     1561 cggacagcgc gtccacgaac tgatcggcct ccaggtgttc acgcatccgc gacgatgcga
     1621 ggtcaacctg ccttgccaaa gcctcctctt cattgatcct gatcaactgc gcatcgacgg
     1681 cgtcgtcgga agggccgcct ttgcgctgct cgatcctgga gatgttggcg gcccgccggt
     1741 aggcaagcaa caagtcgctg ccgtcggcac ttcgcaggaa ctgcgccagc gcgtcggtgc
     1801 gggcgaccag ccgcatgatg tcgtcatcag gatgcgcggc aaagatcgct gccagcacat
     1861 cataacgggc accttcagtc cggagctgta cgcgaaggcg ctcggcgagg aaatccagca
     1921 cctcctgcgg cggcgccgga tgcacgccac ggtgatgcca ggcctcgccc gccagcgtga
     1981 tcagcggcag caggttcagc cgcagcccgt tctcgcgcac gatgcggatg atgccgagcg
     2041 ccgcccgccg caacgcatag ggatcgccgc cgccggtcgg acgctcgccg atggcgaaga
     2101 aacccaccag caggtcgagc ttgtcggcga gagcgacagc gatcgacacg tccgacgtcg
     2161 gcactgcgtc gttcggaccc tttggcgcat aatggtcgcg gatggcatcg gcgacgagcg
     2221 ggtcctcacc atcatgcagc gcgtagtagc ggcccatcac gccttggagc tctgggaact
     2281 cgcccaccat gccggtcgtc aggtcggctt tcgccagccg tgccgcctgc cgtgccaggg
     2341 cagggccggc gcccaccagt ggcgcgatct cctctgccag acgctccagc cgccgaaccc
     2401 gttcgccctg gctgcccagc ttcgcgtgga aggacacgtt gtcgagcagc ggcacccgcg
     2461 attcgagccg cgctctacgg tccatgtccc agaaatggcg cgcatcggca aaacgggcgc
     2521 gcagcacccg ttcgttgccg gcggtggaag cggcgccgcc gtctgcgggc tcgatattgg
     2581 cgacgaagcc gaaccagggc gccgccgaac catccgggtt gcggaaggcg aagtagcgct
     2641 ggttgacgcg catggacacc tgccggactt cggccggaag gtccatgtag gcatcgtcga
     2701 tacggccgag caacggcaga ggccactcca ccagtcccgc cacctcgtcc agtagtgccg
     2761 gatcatccac cagcgcgatc tgccgctccg ccgcgagcgc ggcaatgccg tcggcgatca
     2821 accgcttgcg atcgtctgca tccaccagca cgcggcgttc gcggagcttt gccgaccagt
     2881 cggcggctga cgaaactgtc acggcaccgg gagcaaggaa gcggtggcct tcggtcaggt
     2941 agcctgaggc caggccatgg ccgtcatcgg cgccgtcgcg cagattgaac gggaccacct
     3001 ggccgtccag caagcaaacg atccgtcgca acggccgcac ccagacaaag ttgctggttc
     3061 cgccccaacg catcgacttc ggccagggga agcggcgtag cagcggcggg atcgcggcag
     3121 cgatcagaga cgcggcggga acggcggcgg aagacttttc gagcacccaa taatcgccat
     3181 cctgccgcaa ctgctcgcgg ccggcattgt gcttgcggag aaatccggtc agcgcctgct
     3241 ccggagcact ggtgcgcggg ccgcgctcgg cgctactggc ggcggcaacc tggggcgcta
     3301 cttcgccggc gaacgcgatc cggcgcgggc cgtgataagt ccggatgccg gacggcgaca
     3361 gcgccgcgag cgcctcggag gcctgccgcg cgagatcctc cgcggcgcgg gattgcatcc
     3421 gcgcggggat ttcctcgctg aacagttcca ggaacagttc cggcatgctc agtccttcag
     3481 cgccggttcg atgtcagccg agaggcaatt ttgaccccgt tcttcccctt cggtcccaaa
     3541 agacaggcct cgtggctttc gatgttcagt ccgtcgtcca tgcggtcgcc ttggtgggga
     3601 ggccaattgc cgggtagaac tagtgatcca tccgaccagt gtcgagcacc ggtgcttccg
     3661 gtgacgtgac aacattgccc aacaaccagt catggcgctt gcatgcgata gttttatttt
     3721 caaacttgga gaaggatcct acgccgcgct cagcgcgatc tcgcgggtga tcgccatgat
     3781 gcgctcatgc cggcgccgac gggacagcgc gcgatcgcgt tcttcgcgca gcgcagtcac
     3841 gaccgccaaa cggaggctat ggcccgtcag ttccgccaac tccgctgcaa gttggcaggc
     3901 ctcggc
//
LOCUS       DATGEM010000561         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1396546, whole genome shotgun sequence.
ACCESSION   DATGEM010000561 DATGEM010000000
VERSION     DATGEM010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396546"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VKQ27_17205"
     CDS             <1..587
                     /locus_tag="VKQ27_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HLY90723.1"
                     /translation="MAPSDEAELVHAVATAVAIDDRPSALRYPRGDGVGVALPERGQV
                     MPLGKGRIVREGNNIAILSLGPRLADAMKAADELAARGLPATVVDARFAKPIDGALVE
                     QLARHHEVLITIEEGSIGGFGSFVLHHLAWKGLLDNGLKVRPMILPDIFIDHDSQAKQ
                     MAVAKLSAKDIVATALSAMGIGEALTGGTAITAR"
     gene            606..1826
                     /locus_tag="VKQ27_17210"
     CDS             606..1826
                     /locus_tag="VKQ27_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY90724.1"
                     /translation="MIVAGITAGVSAIGLAQAFAGWIVVARFVGRVVTARSGRPRAAS
                     AGAPVSVLKPLHGDEPLLEEALASTCGQDYAAFQIVFGVSAASDTSLPVVERLRARFP
                     KVDIAVVVDPTLHGENRKVGNLINMLPAAKYDVLVIADSDVHAMPDWLRLLVEALDEP
                     QVGLVTAAYAGVAAHHQVASDTSGRRVSPVPAPPLFQGSGRLASVVGSFGAVQINQYF
                     LPGALLARAMGRQDCLGATMMFRRETLERIGGFHALVDHLADDNVLGRLVQRLGLKVA
                     MAKTIPATTVPEATPPALWRHELRWARTIRALAPIPFAASVLQYPLAWAALTVLLAGN
                     TLWSLVWFALAWALRALAAHGIDRALGLANRSPLWLLPVRELMSVAVMAVSYAGRRVS
                     WRGHTLQAEGFNAQ"
     gene            1823..>2597
                     /locus_tag="VKQ27_17215"
     CDS             1823..>2597
                     /locus_tag="VKQ27_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HLY90725.1"
                     /translation="MMKTLFLHPPSFDGFDGGAGSRYQARREIRSFWYPTWLAQPAAL
                     VPDSKLIDAPPAGVRLDAVTAQARDFELCVIHTSTPSFASDVKVAAALKDANPALKIG
                     FVGAKVAVQPDESLRQGAPIDFVARNEFDFTVKEIAEGRDWASVDGISYRDGAGGIVH
                     NQDRAILEDMDQLPFVTEVYKRDLRIEDYFIGYLLHPYVSLYTGRGCKSRCTFCLWPQ
                     TVGGHRYRTRSVGHVIDEIRWAKAAFPQVKEFFFDDDTFT"
BASE COUNT          410 a          850 c          873 g          464 t
ORIGIN      
        1 tgatggcgcc gtcagacgag gcagaactgg tgcacgccgt cgcaactgct gtcgcgatcg
       61 atgatcgccc cagtgcgctt cgctatccgc gcggcgatgg cgtgggcgtg gcgctgccgg
      121 agcgcggcca ggtgatgcca ctgggcaagg gccgtatcgt gcgcgagggc aacaacatcg
      181 cgatcctgtc gctcgggccc cgtctcgccg atgccatgaa ggcggctgac gaactggccg
      241 cacgggggct gccggcgacc gtcgtcgatg cacgcttcgc caagccgatc gacggggcgc
      301 tggtggaaca attggcccgg caccacgaag tgctgatcac gatcgaggaa ggctccatcg
      361 gcggcttcgg ctcgttcgtg ctgcatcacc ttgcgtggaa gggcctgctc gataacggcc
      421 tgaaggtgcg gccgatgatc ctgccggaca ttttcatcga ccacgacagt caggcgaagc
      481 agatggcggt ggccaagctg agcgcgaagg acatcgtggc cacggcattg tcggcgatgg
      541 gcattggcga ggcgctgact ggcgggacag cgatcaccgc gcgatagaag cccgcttgcg
      601 gcgggatgat cgtcgctggc attaccgcag gtgtctcggc gatcggtttg gcgcaggcgt
      661 ttgccggctg gatcgttgtc gcgcggttcg tcgggcgtgt cgtcaccgcg cggtccggcc
      721 gaccgcgtgc cgcatcggcc ggcgcgccag tcagcgtcct caagccgctg cacggtgacg
      781 aaccgctgct ggaggaagcg ctcgcaagca cctgcgggca ggattatgcc gccttccaga
      841 tcgtgttcgg ggtgtcggcg gcaagcgata ccagtctccc ggtggtggag cgattgcgcg
      901 cgcgctttcc gaaggtggac atcgccgtcg tggtcgaccc caccttgcac ggggaaaacc
      961 gcaaggtcgg caatctgatc aacatgctgc cggcggcgaa atacgacgtg ctggtgatcg
     1021 ccgattcgga tgtgcacgcg atgccggact ggctgcgcct gctggtggag gcgctggatg
     1081 aaccccaggt cgggctggtg actgcagcct atgccggcgt cgcggcccac caccaggttg
     1141 caagcgatac gtccggccgc agggtcagcc ctgtccccgc ccccccttta tttcagggca
     1201 gcggcaggct cgccagcgtg gttggaagct tcggcgcggt gcagatcaac cagtatttcc
     1261 tgcccggcgc gctgctcgca cgggcaatgg gacgccagga ctgcctgggc gccaccatga
     1321 tgttccgccg cgagacgctg gaacggatcg gcggtttcca tgctctggtc gaccatctgg
     1381 ccgacgacaa cgtcctgggt cgtctcgttc agcgcctcgg cctgaaggtg gcaatggcca
     1441 agaccattcc cgcgaccacc gtgcctgaag ccaccccccc tgccctgtgg cgccatgaac
     1501 tccgctgggc acgcacgatc cgcgcactgg caccgatacc gtttgcggct tccgtgctgc
     1561 aatatccgct cgcctgggcc gccctcacgg tgctgctggc aggcaatacg ctgtggtcgc
     1621 tggtctggtt cgcgctggcc tgggcgctac gggcgctcgc cgcgcacgga atcgacaggg
     1681 cgttgggcct tgccaatcgc tcgcccctct ggcttctgcc ggtgcgcgag cttatgtccg
     1741 tggcagtcat ggccgtgagc tatgccggcc gccgcgtcag ctggcgcggc cacacattgc
     1801 aagctgaggg gttcaacgcc caatgatgaa gacgctgttc ctgcatccgc cgtcattcga
     1861 cgggttcgac gggggcgccg gctcgcgcta ccaggcccgg cgggagatcc gcagcttctg
     1921 gtatccgacc tggctcgcgc agccggccgc gctggtgccg gacagcaagc tgatcgatgc
     1981 acctcccgcg ggcgtccgcc tcgacgcggt taccgcccag gcacgcgatt tcgagctgtg
     2041 cgtaatccac acctctacgc cgtccttcgc ctccgacgtg aaggtcgcgg cggcgctgaa
     2101 ggatgcgaac ccggcgctga agattggctt cgtcggcgcc aaggtcgccg tgcagcctga
     2161 cgaaagcctg cggcagggcg cgccgatcga cttcgtagca cgcaacgagt tcgacttcac
     2221 cgtgaaagag atcgccgagg gacgcgactg ggcgtcggtg gacggtattt cctatcgcga
     2281 tggcgccggc gggatcgtgc acaaccagga ccgtgcgatc ctcgaagaca tggatcagct
     2341 tcccttcgtc accgaggtct acaagcgcga cctgcgcatc gaggactact tcatcggcta
     2401 tctgctgcat ccctacgttt cgctgtacac cggccgtggc tgcaaatcgc gctgcacctt
     2461 ctgcctgtgg cctcagacgg tgggcggcca ccgctaccgc acgcgcagcg tcggccatgt
     2521 catcgacgag atccgctggg cgaaagcggc gtttccgcag gtcaaggaat tctttttcga
     2581 cgacgacacc tttaccg
//
LOCUS       DATGEM010000562         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_474167, whole genome shotgun sequence.
ACCESSION   DATGEM010000562 DATGEM010000000
VERSION     DATGEM010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474167"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VKQ27_17220"
     CDS             complement(<1..774)
                     /locus_tag="VKQ27_17220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90726.1"
                     /translation="MRREAWFVSYGLLGLTQNGLVPVLMPLAAPSGAAAGLTYAVFSL
                     LGLFAPVLGSWADYTGRHRDLLIWGTIGAGLMLLPFPWASEPVRIVLAAGAGLGAMAT
                     TTAGNVLAIQDVPEAEWDARVALLQRYVSAGQVIGLLGAGVLAQSHAARGFVVAGIVL
                     LAAGLLAKISAPGRVPRDADAKPAPRPMVGGDAGISGPHHHGHHVSWEELRAYLSVIN
                     QPLRRFLIVWLVAYPAMNGFATLFPVAMTHQFGMVPILPS"
     gene            complement(782..1720)
                     /locus_tag="VKQ27_17225"
     CDS             complement(782..1720)
                     /locus_tag="VKQ27_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLY90727.1"
                     /translation="MKPEILLIEPMTRDVEVQLDGAYTVHRLSQSQDHMGFIADVGAN
                     VRAIVTGGRTGAGNAIVDALPRLEIIAINGIGTDAVDLERARKRGIHVTTTPDVLTDD
                     VADLALALLLATARRVCVGDRFVRAGGWLRGGIPLATKVSGKQLGIIGLGRVGREIAR
                     RAEGFGLSIAYADPLRKEDVSYRYQPDLRCLAGEVDFLIVAAAGGPASRNIVDRAVLD
                     ALGPKGVLINVARGSIVDEVALVAALVDGRLGGAGLDVFAHEPEVPDVLWRLDNVVLQ
                     PHQASATVETRRAMADLVLANLAAHFAGQALPSAVV"
     gene            complement(1717..3000)
                     /locus_tag="VKQ27_17230"
     CDS             complement(1717..3000)
                     /locus_tag="VKQ27_17230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90728.1"
                     /translation="MAAGNIYSRRWARLVPIAFITYSLAYVDRANYSIGSAGGLASDL
                     NITGQANALLGALFFFGYFLFQIPAAWYAETRSAKRLMFWSLLVWGVLAALTGMITNL
                     YLLYLDRFLLGVAESVVLPAMLILLSHWFTSAERSRTNTFLILGNPVTVLWMSIVSGY
                     LVNAIGWRGMFIVEGLPAIIWAFLWLRLVQDEPAQAGWLDPRERDAVEQGLRDEQRAL
                     KPMRNYAEAFRSFHVIALAAQYFLWSIGVYGFVLWLPSMLKASSHFGIVEVGWLSAAP
                     YLLATLLMIVTSHFSDRSGHRKAYIWPFLMVGAVAFAGSYLLGRSNFWPGFILLTIAG
                     GAMYAPYGPFFAWIPETLPRNVAGGAIALINSCGALGSFVGSYVIGWLDAATGGPSLS
                     YALMAAALLLSGLITLAVGTPTQIRLSIPAEGHKA"
BASE COUNT          755 a         1158 c          996 g          517 t
ORIGIN      
        1 cgaaggcagg atcggcacca tgccgaactg gtgcgtcatc gccaccggga acaacgtggc
       61 aaagccgttc atcgccggat acgcgaccag ccacacgatc aggaaccgcc gcagcggctg
      121 gttgatcacg ctgagatagg cacgcagttc ctcccacgac acatgatggc cgtgatgatg
      181 tggaccggag atgccggcat cgccacccac catcggtcgt ggcgcaggct tcgcgtccgc
      241 atcccgtggc acccgccctg gtgcagagat cttcgccaac aagcctgccg ccaacagcac
      301 gatgccagcg acaacaaagc cccgtgcggc atggctctgt gccagcacac cagcccccag
      361 cagaccgatc acctgtcccg cgctgacata gcgttggagc aacgcaacac gcgcgtccca
      421 ctcggcctcc gggacatcct gtatcgccag cacattgcct gccgtcgtcg tcgccattgc
      481 gccaagcccg gcacctgcgg cgagcacaat gcgcaccggt tcggatgccc aagggaatgg
      541 caacagcatc agaccggcgc cgatggttcc ccagatcaac aggtcacgat gccgtcccgt
      601 gtaatcggcc cagctcccca gcaccggcgc gaacagtccg agcagcgaga acactgcata
      661 ggtcaggcct gccgccgctc cgctcggcgc ggccaggggc atcagcaccg gcacgaggcc
      721 gttctgcgtc aatccaagca gaccataaga gacgaaccac gcttcccggc gcaagacgag
      781 atcaaaccac cgctgatggt aatgcctgtc ccgcaaaatg cgccgccagg ttggccagca
      841 ccagatcggc catcgctcgc cgcgtctcca cggtggcgct tgcctggtgc ggctggagca
      901 ccacgttgtc gagacgccac agcacatccg gcacctccgg ctcgtgcgcg aacacatcga
      961 ggccggcgcc acccagccgt ccgtccacca gtgccgcgac cagcgctacc tcatcgacta
     1021 tgctgccgcg cgcgacgttg atcaggacac ccttaggccc caacgcatcc agcaccgcgc
     1081 gatcgacgat attgcgcgac gcaggaccac ccgctgcagc cacgatcagg aaatcgacct
     1141 cgcccgcaag acaacgcaga tccggctggt aacgatacga gacatcctcc ttccggagcg
     1201 gatctgcata ggcgatcgag agtccgaacc cttcggcgcg gcgcgcaatc tcccgaccga
     1261 cacggccaag tccaatgatg ccgagctgct tgccgctcac cttcgtcgcc agcggaatac
     1321 ccccgcgcag ccaaccgccg gcacgtacaa agcgatcgcc gacacagacc cgccgcgccg
     1381 tcgccagcag cagcgcaagc gcaagatcgg cgacatcatc ggtcagcaca tccggcgtcg
     1441 tggtaacgtg aatgccacgc ttgcgcgctc gctccagatc aaccgcatcg gtgccgatac
     1501 cgttgatggc gatgatctcc agtcttggca gcgcatccac gatggcgttg cctgcgcctg
     1561 tccgcccccc cgtcacgatc gcacggacat ttgcgccgac atcggcgata aagcccatat
     1621 gatcttgcga ctgagacagc cgatgtacgg tatacgcgcc gtcgagctgg acctcgacat
     1681 cacgcgtcat cggctcaatc agcaagatct ccggcttcat gccttgtgcc cctccgctgg
     1741 tatggacaga cgtatctgcg tcggcgtacc gaccgcgagc gtgatcaggc cgctgagcaa
     1801 tagcgccgcc gccatcagag cataggacag gcttggcccg cccgtcgccg catccagcca
     1861 gccaatgacg taagacccga cgaaggatcc aagcgcaccg cagctattga tcagtgcgat
     1921 cgctccaccc gcgacgttgc ggggcaaggt ctccgggatc caggcgaaga acggaccgta
     1981 cggcgcatac atcgcgccac cggcgatcgt cagcaggata aagccaggcc agaagtttga
     2041 gcggccaagc agatacgacc cggcgaatgc gacggcacca accatcagga acggccagat
     2101 atatgccttg cgatgaccgg accggtcgga gaagtgtgac gtcacgatca tcagcagcgt
     2161 ggccagcagg taaggcgcag cggacagcca gccgacttcc acgatgccga agtggctgct
     2221 cgccttcagc atcgacggca gccacagcac gaagccatac acaccaatgc tccacaggaa
     2281 gtactgcgcc gcgagcgcga taacgtggaa cgaacggaac gcttccgcat aattgcgcat
     2341 cggcttcaat gcgcgctgtt catcgcgcag gccctgctcg accgcgtcgc gctcgcgcgg
     2401 atctagccaa ccggcctgcg cgggttcgtc ctgcaccagg cgcagccaaa ggaaagccca
     2461 gataatggcg ggcaaaccct cgacgatgaa catgccgcgc caaccgatgg cattcaccag
     2521 ataacccgag acaatggaca tccacagcac cgtgaccgga ttgcccagga tcaggaacgt
     2581 gttggtgcga gaacgttcag cgctggtgaa ccaatggctg aggagtatca gcatggccgg
     2641 cagcacgaca ctttcggcga cgcccagcag gaagcgatcg aggtaaagca ggtaaaggtt
     2701 cgtgatcatg ccggtcagcg cggccagcac gccccacacc agcaggctcc agaacatcag
     2761 gcgtttggca ctgcgcgtct cggcgtacca ggcagccggg atctggaaca ggaaataacc
     2821 gaagaaaaac agcgcgccga gcagtgcatt cgcttggccg gtgatgttca ggtcggacgc
     2881 gagtccgccg gccgaaccga tgctgtaatt cgcccgatcc acataggcca ggctataggt
     2941 gatgaacgcg atcggtacca gccgcgccca acggcggctg tagatgttcc ccgcggccat
     3001 cgaccgtttc ctcgccatga ttgccctaag gaggaatggg cattacccgc ggccgatcaa
     3061 cgcgctcaca tgcctttgct gccggccgcg gcataatcgt tcagcgtagc gatcgtatta
     3121 ttcaacgact cgggccgccg gggtcccgac agcgtagccc gcgacctgac attggcgaaa
     3181 cagacgtgat tgtgcgtaaa aagatacaaa ttctgcctct tgggacgaaa tgctcattga
     3241 cgccatcatc gtcgtgagcc aatttcgcga cacgaatgcg aaacgatgat ctggctgaaa
     3301 caagtgaccg cacgggagac gacgacctgg gcaaggacgg ggaactgatg cccacttttc
     3361 gtgggggcgc cgctggcgag cggagccgct tatttgcggg aatcgagcgg ctcggccaac
     3421 aagccg
//
LOCUS       DATGEM010000563         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_377879, whole genome shotgun sequence.
ACCESSION   DATGEM010000563 DATGEM010000000
VERSION     DATGEM010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377879"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VKQ27_17235"
     CDS             complement(<1..1269)
                     /locus_tag="VKQ27_17235"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLY90729.1"
                     /translation="MTRYVFITGGVVSSLGKGIASASLGALLQARGFSVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVSATRFDNATTGRIYSDVIAKE
                     RRGDYLGATVQVIPHITDAIKEAILHDTRDFDFVLVEIGGTVGDIESLPFLEAIRQLG
                     NELGPERAMFVHLTLVPYIPSAGELKTKPTQHSVKELLNVGIQPQMLLCRCDRPIPEN
                     ERRKIALFCNVRPQAVIAALDVDTIYAVPVSYHAEGMDREVLRHFDLPSERAPDLSRW
                     KGIVDAVRNPEGEVRIAVVGKYTNLLDSYKSLAEALVHGGIANRVKVQLEWVDSEVFE
                     KPDGAVHRLEGVNGILVPGGFGERGTPGKIEAVRFARERAVPFFGICFGMQMAVIEAA
                     RNLIGLRCASSTEFGPCDEPVVGLLTEWARG"
     gene            complement(1365..1709)
                     /gene="secG"
                     /locus_tag="VKQ27_17240"
     CDS             complement(1365..1709)
                     /gene="secG"
                     /locus_tag="VKQ27_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLY90730.1"
                     /translation="MTAVLLIIHLFVTIALIGVVLIQRSEGGGLGIGSSQGMGAFMSG
                     RGTANLLTRMTAILATIFMALSLTLALMNRGTSGVGGGLLANPPPASSAPASPATPAP
                     PPAPKGPSVPTN"
     gene            complement(1771..3414)
                     /locus_tag="VKQ27_17245"
     CDS             complement(1771..3414)
                     /locus_tag="VKQ27_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY90731.1"
                     /translation="MAQPEHQEKPQPDVLRRQELELEQARVALEDAHAEIAALADENS
                     ALRHRLELVEAARNEAEKVARCARDDAARSMGLITRSLSTTTDEKGARVALEEASVLA
                     EELRATNEALVQANDELEQRVADRTLALDQANTKLERLNAHLRVRIEAETAARGKAQA
                     ELFQMQKLEAIGQLTGGIAHDFNNLLTVITSGLQLLARPRDAAHHDRLLGRIQDAAWR
                     GAQLTQRLLAFARRQPLHPERLDLQQHMESMRSLLAHALREDIEMILEVGPDVWPVEA
                     DIAALELALLNLAVNARDAMPAGGRLVLAACNEPTPNGSGGRLDLAPGDYVRLSLSDN
                     GIGMAPEVLDKVFEPFFSTKGDGKGTGLGLAQVYGFARQSGGGAVVESASGKGSTVHI
                     LLPRSHRPPVAVPSLVAAETSEPHQYDHLKILVVEDDDSVASMVLDMLAELGHSGMRV
                     ATVASALALLMSTYDFDLVFSDVLLPGGGSGLDLAREMSRRGLAVPLILTSGFGGGVT
                     QRLAAANLPFLRKPYRIEALNQTIGAAMQSARRSVAGAV"
     gene            3518..3859
                     /locus_tag="VKQ27_17250"
     CDS             3518..3859
                     /locus_tag="VKQ27_17250"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HLY90732.1"
                     /translation="MAEKVAAGHDIIVIGGSAGAFEALKRICSELPADFPAAVFVVIH
                     ISPHSRSVMPDLLSRVGRLPARHPEDEEPIRTGTIYVAPPDLHMLLRSGRIIMSCYPS
                     LDHPAGRLGPS"
BASE COUNT          673 a         1370 c         1167 g          703 t
ORIGIN      
        1 gccgcgcgcc cactcggtca acagccccac caccggttca tcgcacggcc cgaactcggt
       61 ggacgaagcg caccgcaacc cgatcagatt gcgcgccgcc tcgatcaccg ccatctgcat
      121 cccaaagcag atgccgaaga atggcacggc ccgctcgcgc gcgaaccgta ccgcctcgat
      181 ctttcccggc gtaccgcgct caccgaagcc gccgggcacc aggatgccgt tcacgccttc
      241 cagcctatgc acagcgccgt caggcttctc gaagacctcg ctgtccaccc attccagctg
      301 aaccttcacg cgattggcga tgccgccatg caccagcgcc tcggccagcg acttatagct
      361 gtcgagcaga ttggtgtact tgccgaccac cgcgatccgc acctcgcctt ccggattgcg
      421 gaccgcatcg acgatcccct tccagcgcga cagatcaggc gcgcgctccg acggcaggtc
      481 gaaatgccgc agcacttcac ggtccatgcc ctcggcatga tagctcaccg gcaccgcgta
      541 gatcgtatcg acatcgagcg ccgcgatcac cgcctgcggt cgcacattgc agaacagcgc
      601 tatcttgcgg cgctcattct ccgggatcgg cctatcgcac cggcacagca gcatctgcgg
      661 ctggatgccg acattcagca gctccttcac cgaatgctgc gtcggcttcg tcttcagctc
      721 tccagccgat ggaatgtacg gcaccagcgt caggtgcacg aacatcgcgc gctcgggccc
      781 gagctcgttg ccgagctgac ggatcgcctc caggaacggc aggctctcga tgtcgccgac
      841 agttcccccg atctccacca gcacgaaatc gaagtcgcgc gtgtcgtgca ggatggcttc
      901 cttgatcgcg tcggtgatgt gcggaatcac ctgcaccgtc gcacccaggt aatcgccgcg
      961 acgctccttc gcgatcacgt cggaatagat ccgtcccgtg gtcgcgttat cgaagcgggt
     1021 ggcggaaacg ccggtgaacc gctcgtagtg ccccaggtcc aggtcagttt ccgcgccgtc
     1081 atcggtcacg aagacttcgc cgtgctgata cggggacatc gtccctggat cgacattcag
     1141 atacgggtca agcttcctca gccgcaccga gaatccacgc gcttgcagca gcgcgccaag
     1201 cgacgccgag gcaatgcctt tgccgaggga agagaccacg ccgccggtga tgaatacata
     1261 ccgcgtcatg gacggccctt atacgaaccc gaatcggggc aggggaagcc cggatgtgcg
     1321 ccatcacgca tgcgtcacat gcgggcctga aaacccttga aagactaatt cgtcggaacg
     1381 ctcggcccct tgggcgctgg cggcggcgct ggcgtggcgg gcgaggcagg cgccgaggac
     1441 gccggcggcg gattggccag cagccctccg cccacgccgc tcgtcccgcg gttcatcagt
     1501 gccaatgtca gcgacagagc catgaagatc gtggccagga tggcggtcat ccgggtcagg
     1561 agattcgcag tcccccgtcc cgacatgaac gcgcccatcc cttgcgagga gccgatccct
     1621 agcccgcctc cctcgcttcg ctggatcagc acaacgccaa tcagcgcaat cgtgacaaac
     1681 aggtggatga tcagcaggac ggcggtcaaa gtcagggctc ctccgggcgg ggggttctat
     1741 ccccttcccg ccgtccccgg caattgcccc ctaaaccgcg ccggccacgc tgcgccgcgc
     1801 cgactgcatt gcggcgccga tcgtctggtt cagcgcctcg atccggtacg gcttgcgcag
     1861 gaatggcagg ttcgcggcgg ccagccgctg ggtcacgccc ccgccgaagc cgctggtgag
     1921 gatcagcggc accgccagcc cccgccggct catctcgcgc gccagatcca gtccgctgcc
     1981 gccgcccggc agcagcacgt cggaaaacac gaggtcgaag tcatacgtgg acatcagcag
     2041 cgccagcgcc gatgccaccg tcgctaccct catgccgcta tggccgagct ccgcgagcat
     2101 atccaggacc atcgaggcca cgctgtcgtc gtcctccacg accaatatct tcagatggtc
     2161 gtactggtgc ggctccgatg tctccgccgc caccagtgac ggcacagcga ccggcggccg
     2221 atgcgagcgc ggtagcagga tatgcacggt tgagccttta ccggacgcac tctcgaccac
     2281 cgcgccaccg ccggactgcc gggcgaagcc atagacctgt gccaggccga ggccggtacc
     2341 cttcccgtct cccttggtgc tgaagaacgg ctcgaagacc ttgtccagca cctcgggcgc
     2401 catgccgatg ccgttatcgc tgaggctgag gcgaacgtaa tcgcctggcg cgaggtccag
     2461 ccgtccgcca ctcccgtttg gtgtcggttc gttgcatgcc gccagcacca gccgcccgcc
     2521 ggcgggcatc gcatcgcgcg cgttcacggc gaggttgagc agcgccagtt ccagggcggc
     2581 gatgtcggct tcaaccggcc acacgtcggg accgacctcc aggatcatct cgatgtcctc
     2641 gcgcagcgcg tgcgcgagca gcgaacgcat gctctccatg tgctgctgca aatcgaggcg
     2701 ctccggatgc agcggctgcc ggcgggcaaa ggcaagcagc cgctgggtca attgcgctcc
     2761 ccgccaggcc gcatcctgga tgcgccccag aaggcggtca tgatgcgctg cgtcgcgggg
     2821 acgtgccaga agctgcagac cgctggtgat gacggtcagc agattgttaa agtcgtgtgc
     2881 gatgccgccg gtaagctgac cgatcgcctc cagtttctgc atctggaaca actcggcctg
     2941 cgccttgccg cgcgcggcgg tctcggcttc gatccgcacc cggagatgtg cgttcagccg
     3001 ctccagtttt gtgttggcct ggtcgagggc aagtgtcctg tcggcgaccc gttgttccag
     3061 ctcgtcattg gcctgcacca gggcctcgtt ggtcgcccgc aattcctcgg caagcacaga
     3121 ggcctcttcc agagcgacac gcgctccctt ctcgtcggtg gtcgtggaca acgatcgggt
     3181 gatcagcccc atcgaccgcg cggcatcatc gcgcgcgcac cgcgccacct tttccgcttc
     3241 atttcgagcg gcttcaacca gctctagccg atgccgcaac gcgctattct cgtcggcgag
     3301 tgcagcgatc tcggcatgcg catcttccag cgcaacgcgc gcctgctcta actccagttc
     3361 ctggcggcga aggacatccg gttggggctt ttcctggtgc tctggctgag ccatatctca
     3421 ccgacttacg gcagcaacct acgtcgggcc gctggttcgc gaacctcttg aacgcaccgc
     3481 gaggctacac gttgctgtga cagcggggaa ttatgacatg gcggaaaagg ttgcggccgg
     3541 ccacgacatc atcgttatcg gtggctctgc cggcgccttt gaagccttga agcggatctg
     3601 ctccgaattg cccgcggatt ttccggcagc ggtttttgtc gttattcaca tcagcccgca
     3661 ttcccgcagc gtcatgcccg atctgctgag ccgggttggc cgcctgcctg cgcgacatcc
     3721 ggaagatgaa gaacccatca ggacagggac gatttatgta gcgccccccg acctgcacat
     3781 gctcctgcgt agcggtcgca ttattatgag ctgctaccct tctttggacc acccagctgg
     3841 tcgtctcggg ccgtcttgag ctgctaccct tctttggacc acccagctgg tcgtctcggg
     3901 ccgtctttcg gtc
//
LOCUS       DATGEM010000564         3303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_437449, whole genome shotgun sequence.
ACCESSION   DATGEM010000564 DATGEM010000000
VERSION     DATGEM010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437449"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..532
                     /locus_tag="VKQ27_17255"
     CDS             119..532
                     /locus_tag="VKQ27_17255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLY90733.1"
                     /translation="MSEPLTKEELQERLSHSPFIRFQGLIVQEADPERQRVVMIAPMR
                     PEWERIAGSGQWHGGPIAAVIDTVGDYALVMTAGRPLPTINYRVDYIRPAVNTALVVT
                     ATVRRAGRSVGVVDVDVMDEKGSLVAIGRATYAMT"
     gene            complement(622..1473)
                     /locus_tag="VKQ27_17260"
     CDS             complement(622..1473)
                     /locus_tag="VKQ27_17260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HLY90734.1"
                     /translation="MQFGAAMFFTDYSMPPGDLGRAMEERGFESLWAPEHSHIPVSRR
                     SPFPNGGDLPKQYYDVMDPFVTLTAAAAATKTLKVGTGVCLVQQRDPIQTAKLVASVD
                     QVSGGRFLFGVGSGWNAEEMEDHGTPFKSRHKVARERVEAMKIIWTQSKAEYHGEFVN
                     FDPMMTWPKPVQKPHPPVIVGGAFPYGARRAIRYGDGWVPHAGRPEYEDVSVFLPQFH
                     QLVKEAGRDITSLPVTMFRVVEELEKLRHYRDIGIARVVITLPSSGAGDVLPILDRWA
                     EKIRRMG"
     gene            complement(1587..2315)
                     /locus_tag="VKQ27_17265"
     CDS             complement(1587..2315)
                     /locus_tag="VKQ27_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HLY90735.1"
                     /translation="MFIHRPDPIHKVRVETPDPVFAEKLLEQFGGATGELSAALTYFT
                     QSFHTEDKGIRDMLQNIATEEFGHLEMVALMIEQHTKRAKQGAQEKAFGSTLFAMRGY
                     GPHLVDSRGSTWDARYVNEGGSVVRDLRADIAAEAGALVTYEALIHQAPDEGSREALR
                     HLATREVAHARMFMKALESMNKLDDPMFGDLKPDDTVDVYFNLTTGPEAERRGPWNSE
                     PEFHIRDLTAQAQRGGGASAPPKR"
     gene            2426..>3303
                     /locus_tag="VKQ27_17270"
     CDS             2426..>3303
                     /locus_tag="VKQ27_17270"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY90736.1"
                     /translation="MRVGIEVGGTFTDLVAVDGDRVIVGKVPSTPASPDVGAFAALTA
                     ADIAPARVTDLVHGSTVATNAILERKGARIAFVTTRGFRDIPFLQRHDRRNIYDLRYA
                     KPQPPVARRDCFEVSERIDAAGTVVVPLDEGSIRRELLPALARGGYQAVAICLLSAYV
                     APQHEARLRALIREAMPELRIACSHEIAREFREFERASTTLLSAYVQPVIDRYLDRFA
                     TQLGEGGFSGRFSVMQSNGGRLPAAAMRGNAITALFSGPAAGVIGAIRQAARSGCGDL
                     ITFDMGGTSTDVSLVE"
BASE COUNT          574 a          999 c         1095 g          635 t
ORIGIN      
        1 tggtgcgcga agggcgactg aacacgctgg ttgagcagac gtatccgctg gcacaggcag
       61 cggaagcact gcgcgtgatc gaggatcgcg aggtgttcgg caagatcgtg gtcacgccat
      121 gagcgaacca ctgaccaagg aagagttgca ggaacggctg agccattcac cgttcatccg
      181 attccagggg ttgatcgtgc aggaagccga tccggagcgg caacgcgtgg tcatgatcgc
      241 gccgatgagg ccggagtggg agcggatcgc tggcagcggg cagtggcatg gtgggccgat
      301 cgctgcggtg atcgacacgg tgggtgacta tgcacttgtt atgacggccg gccgaccgct
      361 tccaacaatc aactaccgtg tggattatat tcggccagcg gtgaataccg cgctggtggt
      421 taccgcgacg gtgcggcggg cgggtcgaag cgtcggcgtg gtggatgtgg atgtgatgga
      481 tgagaagggc tcgctggtag cgatcgggcg ggccacctac gcgatgacct gactgcctcc
      541 cctgtgtcat tgtgagcgca gccaagcaat cccggacgag attgcttcgt cgctttgctc
      601 ctcgcaatga caagggtggg gtcagcccat ccggcggatt ttttctgccc agcggtcgag
      661 gatcggcaga acgtcccctg cgccggagga gggcaacgtg atcaccacgc gcgcgatgcc
      721 gatgtcgcgg tagtggcgaa gcttttccag ctcttcgaca acgcggaaca ttgtcaccgg
      781 caaggacgtg atgtcgcgac ccgcttcttt caccagttgg tggaactgcg gcaggaagac
      841 gctgacgtct tcatactccg ggcggcccgc gtggggcacc caaccgtcgc cgtagcggat
      901 tgcacggcgc gcgccatacg ggaaagcgcc gccgacgatt acgggaggat ggggcttctg
      961 cactggcttc ggccaagtca tcatcggatc gaagttgacg aactcgccgt ggtactcggc
     1021 cttgctttgc gtccagatga tcttcatcgc ctcgacgcgc tcgcgcgcga ccttgtggcg
     1081 tgacttgaag ggcgtgccgt ggtcttccat ttcctcggca ttccatccgg agcccacgcc
     1141 gaacaggaag cgcccaccgg acacctgatc gaccgatgcg accagtttcg ccgtctggat
     1201 cggatcgcgc tgctgcacca ggcagacgcc ggtgccgact ttcaatgtct tcgtcgcggc
     1261 cgctgcagca gtcagtgtga cgaacgggtc catcacgtcg tagtactgct tcggcaggtc
     1321 gccaccattg ggaaacggcg atcgccgtga caccggtata tgggaatgct ccggtgccca
     1381 aagcgactcg aagccgcgct cttccatcgc gcggccgaga tcgccgggcg gcatggaata
     1441 gtcggtgaag aacatggccg cgccgaactg catctggggc tcccccgcat cactgcagag
     1501 gagtctaatc cacgcctgct gtcattgcga gcgcggcgaa gcaatcccga acgagattgc
     1561 ttcgcgctgc tcgcaatgac aggtcatcaa cgcttcggcg gcgcgcttgc gccaccgcct
     1621 cgctgggcct gtgctgtgag gtcacggatg tggaactctg gctcgctgtt ccatggacca
     1681 cgccgttcgg cttccggacc cgtcgtcagg ttgaaataga cgtccacggt gtcgtccggc
     1741 ttcaggtcgc cgaacatcgg gtcgtccagc ttgttcatcg actcgagcgc cttcatgaac
     1801 atgcgtgcat gggcgacttc gcgtgtcgca aggtgccgca gcgcctcgcg cgagccctcg
     1861 tccggagcct ggtggatcag cgcttcatag gtcaccagcg caccggcctc ggctgcgatg
     1921 tcggcgcgca aatcgcgtac gacactgccg ccctcgttca cgtagcgcgc gtcccaggtg
     1981 ctgccgcgtg agtccacaag gtgaggaccg tagccgcgca tcgcaaacag cgtgctgccg
     2041 aatgccttct cctgggcgcc ctgctttgcg cgcttcgtgt gctgctcgat catcagcgcg
     2101 accatttcaa gatggccgaa ctcctcggtc gcgatattct gcagcatgtc gcggatgcct
     2161 ttgtcctcgg tatggaagga ctgggtgaag taggtcagcg cggctgaaag ttcgccagtc
     2221 gctccgccga actgttccag cagcttctcg gcaaacacgg gatcgggagt ctcgacgcgc
     2281 actttatgga tcggatcggg gcgatggatg aacatgggtg agcttccttt cgatgggagc
     2341 agcgtgaccg caacagccgc cccgggtgcg ggttggctgt tcacgagaaa gtgcctctgc
     2401 catgctgcgt gcaggagaac gaagcatgcg ggttggcatc gaagtcggcg gcaccttcac
     2461 ggatctggtc gccgttgacg gagatcgggt cattgtcggc aaggttccga gcacacccgc
     2521 gagtcccgat gtcggtgcat tcgccgcgct gaccgctgcc gacattgcgc ccgcacgtgt
     2581 gaccgatctg gtgcatgggt cgacggtcgc gaccaatgcg atcctagagc gcaagggcgc
     2641 gcgcatcgcc ttcgtcacca cgcgcggctt ccgcgacatc ccgttcctgc aacggcacga
     2701 tcggcgcaat atctacgatc tgcgctacgc caagcctcag ccgcctgtcg cgcgacgcga
     2761 ctgtttcgag gtttcggagc gcatcgatgc cgccggtacg gtggtggtgc cgctggatga
     2821 gggctcgata cggcgagaat tgctgccggc tctggcaagg ggcggctatc aggcggtggc
     2881 aatctgcctg ctgtctgcct acgttgcgcc ccagcatgag gcgcgtttgc gcgcgctgat
     2941 acgcgaggcg atgccggaac tgcgcattgc gtgttcgcac gagatcgcgc gcgagttccg
     3001 cgaattcgag cgcgcaagca cgacgttgct gtccgcctac gtgcagcctg tgatcgaccg
     3061 gtatcttgat cgtttcgcga cgcagcttgg cgaaggaggc ttcagcggcc gtttcagcgt
     3121 aatgcagtca aacggcgggc gattgccggc ggctgcgatg cgcggcaacg cgatcaccgc
     3181 gctgttcagc gggccagccg ccggcgtaat cggcgcgatc cggcaggcgg cacgatcggg
     3241 atgtggcgac ctcatcactt tcgacatggg cggcacatcg accgatgtga gcctggtgga
     3301 aaa
//
LOCUS       DATGEM010000565         4850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1176414, whole genome shotgun sequence.
ACCESSION   DATGEM010000565 DATGEM010000000
VERSION     DATGEM010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176414"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VKQ27_17275"
     CDS             complement(<1..947)
                     /locus_tag="VKQ27_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90737.1"
                     /translation="MAGYLLHRLAGALGVVFAMSVLIFAVVHILPGNVAYAILGEFAT
                     AENVRALEAKLGLNDPLPLQYWHWLSGMLHGDLGASVVMQRPAAPLIVEALGRSAILA
                     ALAFVMVATGGIALGVFAATHQGQLSDRIATLTQLVFIATPEFFWGILAVLLFGSWMQ
                     VLPATGYAPLDEAGFLAWASHLVLPVLVLACGLLAHVSRLTRSAMIEVLQSRYVLAAR
                     AKGFGESRVLWRHALPNALLPAITILAIDFGLLIGGVVVVETVFAYPGLGRLLVFAIE
                     HHDLPLLQAGMMVVTAIYALANLAADLSYAVLNPRIRVGG"
     gene            complement(962..2458)
                     /locus_tag="VKQ27_17280"
     CDS             complement(962..2458)
                     /locus_tag="VKQ27_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90738.1"
                     /translation="MSLTRRLLIGSSLAASIISRARAADGVMRIGISTSLNTLDPLMT
                     STGDEYIFDNICFNGLTRMREDLVVEPDLAESWSFTEDLKRWTFKLRRGVKFHNGDEM
                     VADDVVAMFRRLLDPKVAAPARSQYDMVDTVSAPDPATVVFELKVPYSGLADVLADRQ
                     AKITPRSAVAQMATKPVGTGAFKFVSYTPGDRVVMTRNSDYFDAGLPKIAGAELRIIP
                     EISVRIAALQAGDIDILWDLPLEQVKDFGKQSGLRAESIPTASWDGAVMNNLNPPFNN
                     LRVRQAFHLGVDKRDLVELTLFGQGLPTLSPIPPTHPFYAKDVVIPPADPVAARKMLA
                     EAGHPDGIKMPMIVPIGRPLRERLGVSLQQLLKPAGFDLQIQRVPYSSFSAEVSGKAP
                     LYIDGFFARPTVDTSTFPFLHTGASWNEQLWHYSNPDVDKALETARTSGDVNVQKTSY
                     IAMQKALAAVPPGMFAYVSNFACAFRSNVGGVHTHPMRWFDLRNATLS"
     gene            complement(2531..3841)
                     /locus_tag="VKQ27_17285"
     CDS             complement(2531..3841)
                     /locus_tag="VKQ27_17285"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463388.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HLY90739.1"
                     /translation="MSVAGKMHLVAYLKAGPTASYASAWRYPGAPLNDLWEPERYEHL
                     ARVLEAARFDAGFFADGMGVPDIYKNSFADYLGRGGQLSLLDPLMLLPLMARVTTRLG
                     LGTSLSTTFNTPYPLARVLGSLDLLSKGRAAWNVVTSANNYEAWNCGLDAIPPRDERY
                     DRADEMMEACFALWDCWDPDVMVLDRASGVFADTSKVRYADYEGRHVRTRGPLSIPRS
                     PQGRPVILQAGSSPRGRAFAARWAEIIFCSHATKSDAITFHNDMAQRLEAAGRKPSDC
                     AVLPSLSVVVGETDAIAREKAAFLDELIDPELVIASSSALLGVDLSEIESADAASVGA
                     GDQGVQGSLDRMRQVAKAQGISFAQAVRKPRGLLAGSPATIADYMEDWFTSGACDGFV
                     LPPTVFPATYEEFGRMVVPELQRRGLFRTEYAGRTLRENLQNPG"
     gene            4225..4410
                     /locus_tag="VKQ27_17290"
     CDS             4225..4410
                     /locus_tag="VKQ27_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90740.1"
                     /translation="MHPEDEQAATALACIEMLRHGITTFIEPGSAFEPDAVADATHRP
                     QAYAAGSPTRISGTTHR"
     gene            4617..4808
                     /locus_tag="VKQ27_17295"
     CDS             4617..4808
                     /locus_tag="VKQ27_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90741.1"
                     /translation="MLLNSHLGFDLDLADAMEQRWRRPRFLHLAELGVLGPYTNFVHM
                     NLIRDAEIDPIQRSVLTIV"
BASE COUNT          957 a         1581 c         1496 g          816 t
ORIGIN      
        1 ccgccaacgc ggatgcgcgg gttgagtacc gcgtaactca gatcggcggc gaggttagcc
       61 agcgcataga tcgcggtgac caccatcatg ccggcctgca gcaacggcag gtcgtggtgc
      121 tcgatggcaa acaccagcag ccggccaagc ccgggatagg cgaacacggt ctcgaccacc
      181 acaaccccgc caatcagcag accaaagtcg atggcgagga tcgtgatcgc gggcagcagc
      241 gcatttggca gcgcatggcg ccagagcacg cgtgattcgc caaatccctt tgcccgtgcg
      301 gctagcacgt agcggctttg cagcacctcg atcatcgccg agcgcgtcaa ccgcgacaca
      361 tgcgccagca ggccgcacgc gagcaccagc accggcaaca caagatgcga cgcccaagca
      421 aggaaccctg cttcgtcgag cggcgcgtat cccgtcgccg gcaacacctg catccaggag
      481 ccgaacagca gcaccgccag aatgccccag aagaactccg gcgtcgcgat gaacaccagc
      541 tgcgtcaacg ttgcgatgcg gtcagacagc tgcccctgat gcgtggccgc gaacacacca
      601 agcgcgatgc cgcctgttgc caccatcacg aatgcaagtg ccgccaggat cgccgaacgc
      661 cccaacgcct ccacaatcag tggcgccgct ggccgctgca tcaccactga ggcgcccaga
      721 tcgccgtgca gcatgccgct cagccagtgc cagtattgca gcggcaacgg atcgttcagg
      781 ccaagcttcg cttccagcgc ccgaacgttt tcggctgtgg cgaattcgcc caggatcgca
      841 tacgccacgt tccccggcag gatatgcacc accgcgaaga tcagcaccga catggcgaac
      901 accacgccaa gcgccccggc cagccgatgc agcagatatc cggccaccga tacgccgcga
      961 ctcagctcag cgtggcgttg cgaaggtcaa accagcgcat cgggtgtgta tgcacgccgc
     1021 cgacattgct gcggaaggcg caggcgaagt tcgagacata ggcgaacatg ccaggcggga
     1081 cggctgccag cgccttctgc atggcgatgt agctggtctt ctgcacgttg acatcgccgc
     1141 tggtgcgcgc ggtctccagc gccttgtcga catcggggtt gctgtagtgc caaagctgtt
     1201 cgttccagct ggcaccggtg tggaggaacg ggaaggtact ggtatccacg gtcggccgcg
     1261 cgaagaagcc gtcgatatag agcggcgcct tgccggagac ttcggcggag aagctggaat
     1321 agggcacgcg ctggatctgc agatcgaagc cggccggttt cagcagttgt tgcagcgaga
     1381 cgccgaggcg ttcgcgcaac gggcgtccga tcggcacgat catcggcatc ttgatgccgt
     1441 ccgggtgtcc cgcttcagcc agcatcttgc gtgccgccac cggatcggcc ggcgggatga
     1501 cgacgtcctt ggcgtagaac ggatgcgtcg gcggaatcgg gctcagcgtg ggcagcccct
     1561 ggccgaacag cgtcagctcg accaggtcgc gcttgtcgac gccgaggtga aaggcctggc
     1621 gcaccctgag attgttgaat ggcggattga ggttgttcat gaccgcgccg tcccaggacg
     1681 cagtcggaat gctctcggcg cgaagccctg attgcttgcc gaaatccttc acctgctcga
     1741 gcggcaggtc ccacaatatg tcgatatcgc cagcctgcaa cgcggcgatc cgtacactga
     1801 tctcagggat gatgcgcagc tctgcacctg caatcttggg cagcccggca tcaaagtaat
     1861 cggaattgcg cgtcatcacc acacgatcac caggcgtgta gctgacgaat ttgaatgcgc
     1921 cggtgccgac gggcttcgtc gccatctgtg ccactgcgct gcgcggtgta atcttcgcct
     1981 gccggtccgc cagcacatcg gcgaggccgc tatagggaac cttcagctcg aacaccaccg
     2041 tcgcaggatc gggcgccgag acggtatcga ccatgtcgta ctggctgcgg gccggcgcag
     2101 ccaccttggg gtcgagcaat cggcggaaca tcgcgaccac gtcgtcagcg accatctcgt
     2161 cgccgttgtg gaatttgacg ccgcggcgca gcttgaatgt ccaccgcttg aggtcttcgg
     2221 tgaacgacca gctttcggcg agatccggtt caaccaccag atcctcgcgc atgcgcgtca
     2281 ggccgttgaa acagatattg tcgaatatgt attcatcgcc ggtcgaggtc atcagcgggt
     2341 caagcgtatt gagcgacgtg ctgatgccga tgcgcatgac accgtccgcg gcgcgtgcgc
     2401 gtgatatgat cgatgccgcg agtgacgagc cgatcagcaa cctgcgggtg agcgacatag
     2461 cctatccttc gcatatcggg tttcagaagc ggcgaggcac ttaacttaag caatccgcgt
     2521 gccgggcaat tcagcccggg ttttgcaggt tctcgcgcaa ggtacggcct gcatattcgg
     2581 tgcgaaatag gccgcgccgc tgcaattccg gcacgaccat gcggccgaat tcctcgtatg
     2641 tcgctggaaa aaccgtcggc ggcagcacga agccgtcgca ggcaccgctg gtgaaccagt
     2701 cctccatgta gtcggcgatg gttgccggtg aaccagccag caggccacgt ggtttgcgta
     2761 ccgcttgggc gaaactgata ccctgcgcct tcgccacctg gcgcatccgg tcgagcgatc
     2821 cctgcacgcc ctgatcaccg gcaccgaccg aggcggcgtc ggcggattcg atctcgctca
     2881 gatcgacgcc gagcagagcc gaactcgacg caatcactag ctcggggtcg atcagctcat
     2941 cgaggaacgc ggccttctcg cgcgcgatgg cgtcggtctc gcccaccacg accgaaagcg
     3001 aaggcagcac cgcacagtcg gacggcttgc gtcctgctgc ctccagacgc tgcgccatgt
     3061 cgttgtggaa ggtaatggcg tcgctcttgg tggcgtgcga gcaaaagatg atctccgccc
     3121 atctggcggc aaacgcccgt ccgcgtggcg acgaacccgc ctgcaggatc actggccgtc
     3181 cttgtggact gcgcggaatc gaaagcgggc cgcgcgtgcg aacatggcgg ccctcataat
     3241 cagcgtagcg gaccttcgac gtatcggcaa acacgccgct ggcgcgatcg agcaccatga
     3301 catcggggtc ccagcaatcc catagggcga agcacgcctc catcatctcg tccgcgcggt
     3361 catagcgttc gtctctgggt ggaattgcat ccaggccgca gttccacgcc tcgtagttgt
     3421 tcgccgaggt gacgacattc catgccgcac gccccttgct cagcagatca agcgagccga
     3481 gcacgcgcgc caacggatat ggcgtattga atgtggtgga cagcgatgtg ccgagcccca
     3541 accgtgtcgt cacccgtgcc atcagcggca gcagcatcag cggatcgagc agtgacagct
     3601 gaccgccgcg gccgagatag tcggcgaagc tattcttgta gatatccgga acccccatgc
     3661 catcggcgaa gaagccggcg tcaaaccgtg cggcctcgag cacgcgtgcc agatgctcgt
     3721 agcgctcggg ctcccaaagg tcattaagcg gcgcaccagg ataacgccac gcgctggcat
     3781 agctcgcggt aggccccgcc ttcaggtagg ccaccagatg catcttgccc gcaacgctca
     3841 cgatctgctc cttgaatgat cgaggtcgga ggtagcaaat tctgtaccgc gcacgtcgca
     3901 cacatcaaag gacagaaccg cggatgacag caacttccga gacctgcgat ctccttatcc
     3961 gtaacggcca ggtgctgacg atggatgcgc aacgcacggt gcatccgtgc agcgccatcg
     4021 caatacaggg gcacagcatc gcagcggtcg gaccggaagc agccgtgctg ccgcgatggc
     4081 gatcagcgct cgtgctggat gcccagggcg cgattgtgca tcccggtttt gtcgacgcgc
     4141 atctgcacgt gaacgcgcag acctgccgcg gatcccagca agggcggcac cagcggcccg
     4201 agttatgccg actggaaggc agccttgcac cccgaggacg agcaggcagc gacggcgctt
     4261 gcctgcatcg agatgctgcg tcacggcatc acgacgttca tcgagcccgg cagcgccttc
     4321 gaacctgacg cggttgccga tgcgacacat aggccacagg catacgctgc tggctcgccg
     4381 acccgtatct ctgggacgac acatcgctga tggatgccat cccgggactg cggagcgacc
     4441 aattgctggc gcgcgtgccg gcagaccgcg accggtgctt caaactgctc ggcagctagc
     4501 tggtccgcaa ccgcgacgcc gacggcatcc tgcacggact tgtcgcgcta tatggcgagg
     4561 gaaccgcgtc ggatgagctt ctccaggcgg caaaggcact cgccgaccac gaacgcgtgt
     4621 tactgaacag ccatctcggc ttcgatctcg atctggcgga cgcaatggaa cagcgctggc
     4681 gccggccgcg cttcttgcat ctggccgaac tcggcgtgct cggcccttat acgaacttcg
     4741 tccacatgaa cctgatccgc gacgccgaga tcgatccgat ccaacgctca gtattgacta
     4801 tcgtctgatg ccccgccgcc cacatgacgc gcggcatcgc gatgcgcgag
//
LOCUS       DATGEM010000566         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1580953, whole genome shotgun sequence.
ACCESSION   DATGEM010000566 DATGEM010000000
VERSION     DATGEM010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580953"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VKQ27_17300"
     CDS             complement(<1..791)
                     /locus_tag="VKQ27_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLY90742.1"
                     /translation="MSAVDVKQAPARTLGRVAAVGARRKESVWQQIWRDRIMLLLIAP
                     GLIYFVLFRYIPLLGNVIAFQDYLPFVPIVDSPFVGFANFQSMVSDTAFWQSTANTII
                     IAMCQLIFYFPMPIILAMMLQSLISMRVRRIIQSVVYLPHFISWVIVVALWQQVLGGS
                     GIFVHTLRDVGLPAVNVMTEPGFFKELVTAQLIWKETGYATIIFLAALLSIDSSLYEA
                     AVMDGAGRWQRLWNVTLPGIMGVIILLLILRLGLILSVGFEQILL"
     gene            complement(980..2341)
                     /locus_tag="VKQ27_17305"
     CDS             complement(980..2341)
                     /locus_tag="VKQ27_17305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HLY90743.1"
                     /translation="MDKVVASPEEAVADIPNGASIAISGFGLSSGVPNSLLDAAAKQG
                     ATDLCLVANGVGGAASRLIENDQVSRLIVSFVARPNIESAAADKVTAGTLTFEIVPQG
                     TLVERLRAGGAGLAGIYTPTGVGTPIAHDKEVRFFDGKPFLFEPAIKVDYAFVCAYRA
                     DRLGNCEFRGSAQHFNPSFAKGAKVAIVEVDEIVEPGEINPHRVGLPGSFVARVVKRT
                     IQPDRSGLARFRRAPDTARTYNAKTAWTRHQMAEKAASLLPEPGYVNLGLGIPTLVSN
                     YLGGRDIALHGENGILNYGPPADQDHVDWYIHDAGGGYVTEKPGIAYFDSVTAFEMVR
                     SGKINVVVLGAYQVDEAGNVANWTTPEQVGGGIGGAMDMVAGGATVMVMMEHRDSRDR
                     AKLVRSCTYPLTGAGCVDIVVTDLAVLRRVEGRFRIEDVAPGFTAEEVAVLSEMDLSP
                     TPV"
     gene            2387..>2642
                     /locus_tag="VKQ27_17310"
     CDS             2387..>2642
                     /locus_tag="VKQ27_17310"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY90744.1"
                     /translation="MPAEQAAEALMASMKLNGIERLWFTSGTELAFFQEIPVKHQALG
                     RPTPKIMTMTHENAALSAAMGETMLTGKPSATAAHVECGMI"
BASE COUNT          502 a          789 c          884 g          467 t
ORIGIN      
        1 tggagcagga tctgctcgaa accgaccgac aggatcaggc ccagacgcag gatcagcagc
       61 aggatgatga cgcccatgat gccgggcagc gtgacgttcc agaggcgctg ccagcgaccc
      121 gcaccgtcca taaccgcggc ttcatacagc gaagaatcga tgctgagcag cgcagccagg
      181 aagatgatcg tggcgtagcc cgtctctttc cagatcaact gcgcggtgac cagctccttg
      241 aagaagccgg gctcggtcat gacgttgacg gcgggcaagc cgacgtcgcg cagagtgtgg
      301 acgaagatgc ccgagccacc cagcacctgc tgccagagcg ccacgacgat gacccaggag
      361 atgaagtgcg gcaggtacac gacgctctgg atgatgcgtc gtacgcgcat gctgatcagg
      421 ctctgcagca tcatcgcgag gatgatcggc atcgggaaat agaagatcag ctggcacatg
      481 gcgatgatga tcgtgttcgc ggtggattgc cagaaggccg tgtcgctgac catggattgg
      541 aaattggcga agccgacgaa ggggctgtcg acaattggga cgaacggcag atagtcctga
      601 aaggcgatca cgttgccgag cagcgggatg tagcggaaca ggacgaagta gatgaggcct
      661 ggcgcgatga gcaacaacat gatccggtca cgccagatct gctgccagac gctctccttt
      721 ctgcgcgcac cgacagcggc aacgcgcccc agcgtccgtg cgggagcctg ctttacgtct
      781 acggcactca tgtcgccatc tccgatccac gccgatgatc gacggacagg taaaacgctg
      841 gtccgcgacc cctgaccatc cagcgtgcgg tgcgaggtat ctgcagaacc tgaggatatc
      901 tcgcggcttc agggattgga aagatagtca ataggttcag tgatacgtat tttcgtcgcg
      961 tttttcatac tgatttggcc tagacgggcg tggggctcag gtccatctcc gatagcacgg
     1021 cgacctcttc ggccgtgaag ccgggtgcga cgtcttcgat gcggaagcgg ccctcgacgc
     1081 ggcgcagcac cgccaggtcg gtgaccacga tgtccacgca gccggcgcct gttaacggat
     1141 aggtacacga gcgcaccagc ttggcccgat cgcggctgtc gcgatgctcc atcatcacca
     1201 tgacggtggc gccaccggcg accatgtcca tggcgccgcc gatgccgccg ccgacttgct
     1261 cgggcgtggt ccagttggcc acattgccgg cttcgtcgac ctggtaggcg ccgagcacca
     1321 cgacgttgat cttgccggag cggaccatct cgaaggcggt gacgctgtcg aagtaggcga
     1381 tgcccggttt ctccgtcaca tagccgccgc cggcatcgtg gatgtaccag tcgacgtggt
     1441 cctggtcggc gggcggtccg tagttgagga tcccgttctc gccgtgcagc gcgatgtcgc
     1501 gaccgcccag gtagttcgac acgagtgtcg ggatcccgag gccgaggttc acgtagccag
     1561 gttcgggcaa cagcgaggcg gctttttccg ccatctgatg tcgagtccac gctgttttgg
     1621 cgttgtacgt gcgagcggtg tccggcgcgc gccggaagcg agcgaggcca ctccgatcgg
     1681 gctggatggt gcgcttcacc acgcgtgcga cgaaactgcc tggcagtccg acgcggtgcg
     1741 ggttgatctc gccgggctcg acgatctcgt cgacctcgac gatagcgacc ttcgcgcctt
     1801 tggcgaagct cgggttgaag tgctgggcac tgccgcggaa ctcgcagttg ccgaggcggt
     1861 cggcgcggta ggcgcacacg aaggcgtagt cgaccttgat ggctggctcg aagagaaacg
     1921 gcttgccgtc gaagaaacgc acttccttgt cgtgggcgat gggcgtgccg acgccggttg
     1981 gcgtgtagat gccggcgagt cccgcgccgc cggcgcgcag ccgctcgacg agcgtaccct
     2041 gcgggacgat ctcgaaggtg agcgtgcccg cggtgacttt gtcggcggca gccgactcga
     2101 tgtttgggcg ggcgacgaag gagacgatca ggcgtgagac ctggtcgttt tcgatgagcc
     2161 tggacgcggc gccaccgact ccgttggcaa ccagacacag gtccgttgcg ccctgtttgg
     2221 cggcggcgtc gaggagactg ttcggcacgc ccgagctgag gccgaagcca gagatcgcga
     2281 tcgacgcgcc gttgggaatg tctgcgactg cctcctcggg tgaggccacc accttgtcca
     2341 tcaaaggcgg agcctaccat cgaagactat gcaaccgatg tactccatgc ccgccgaaca
     2401 ggcggctgag gccttgatgg cgtccatgaa gctcaatggc attgagcggc tgtggtttac
     2461 ctcgggcact gaactggcct ttttccagga aatccctgtc aagcaccagg cgctgggccg
     2521 gccgacgccg aagatcatga ccatgacgca cgagaacgcg gcgctctcag cggccatggg
     2581 cgaaacgatg ctgacgggca agccttcggc aacggcggcg cacgtcgagt gcggaatgat
     2641 ca
//
LOCUS       DATGEM010000567         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1444584, whole genome shotgun sequence.
ACCESSION   DATGEM010000567 DATGEM010000000
VERSION     DATGEM010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444584"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..317)
                     /locus_tag="VKQ27_17315"
     CDS             complement(108..317)
                     /locus_tag="VKQ27_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90745.1"
                     /translation="MADTPTPEALAAEFDCLMARAGLTVPPQRRAAALAAFADLRGQI
                     ALLHGRYGYEAEPSNVFSLTPTGAK"
     gene            398..1564
                     /gene="pobA"
                     /locus_tag="VKQ27_17320"
     CDS             398..1564
                     /gene="pobA"
                     /locus_tag="VKQ27_17320"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HLY90746.1"
                     /translation="MRTQVGIVGAGPAGLLLSHLLHLQGIESVVLEARTREYVENRIR
                     AGVLEYGTEQILNDSGVGDRMRQEGMLHPGVVMRFGHADHRIDFADLVGKHVMVYSQH
                     KVVQDLIAARLAGGGQIVFEADDVSVHDFEGDAPRIRYRHAGDVHEVACDIIAGCDGF
                     HGVCRPSIPDHLLRIYERVYPFGWLGILAETPPSTEENVYVSHERGFALLAMRSPTLS
                     RLYLQCAPDEDLAAWSDDRIWSELQTRAATDDFRLAEGPILQRGITPMRSFVTEPMQR
                     GRLYLAGDAAHIVPPTGAKGLNLAAADVAMLTRGIVAFYRDGRTDMLDEYSATCLRRI
                     WKVQRFSWWMTTLLHRFFDHTPFEQQMQAAELDYVFSSRAASLALAENYVGLPL"
     gene            complement(1559..2323)
                     /locus_tag="VKQ27_17325"
     CDS             complement(1559..2323)
                     /locus_tag="VKQ27_17325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90747.1"
                     /translation="MRFKDKVALITAVGSGIGRATADIMAAEGAVIIGVDNDERRLAM
                     AMEAINMSGGRAIGRPTNALDEAQVAEVVAWAARQYGTIDILVNAVGGSTIIGNPGAT
                     LDELSLSDWQRLVAFNLDGTFLFCHAVIPLMKRRKSGKIVNLASIAGRGLSIASSGAY
                     AAAKGGIIALTRKLSLELGPYNINVNAIAPSRTLTERIRPRFNQQSPEDQAAEIERVP
                     LRRMAEAADQAKVICFLASSDADYVTGQTIDVTGGL"
     gene            complement(2347..3072)
                     /locus_tag="VKQ27_17330"
     CDS             complement(2347..3072)
                     /locus_tag="VKQ27_17330"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HLY90748.1"
                     /translation="MARTPITAATIAAELEVTPRTVYRDIATLQSRRVPIDGEAGVGY
                     VLRRGFELPPLMLTEEEVQAVAVGLRLLRRTGDPGLDSAARQVLVKLAAAMPEVLRAQ
                     LLEAPFHVATRGAPSPSAIDLSETRAAIRNAHKMWIDYVDEKGTATSRTICPVAMEYH
                     VEATLICAWCELRDDYRHFRTDRIRGARVLQLDFAARASTLLAGWVELTRSDRQKRED
                     GQPARVAADIAGRAAVDSSLSME"
BASE COUNT          467 a          908 c         1124 g          573 t
ORIGIN      
        1 gcgggcggcg acgcgcgaca aggcgtcgcg ggcaagcgtt tccgaagtga tggtgccggc
       61 gcggagcagg cggcccatct cggcgatgga gaggtcctgc gtttccatca cttcgctcct
      121 gtcggtgtca ggctgaacac gttggacggt tcggcttcat agccgtagcg gccatgcagc
      181 agcgcgatct ggcctcggag gtcggcgaag gcggcgagcg ccgcggcgcg gcgttgcggc
      241 ggcacggtga ggccggcgcg cgccatcagg cagtcgaact cggcagccag tgcctcgggt
      301 gttggtgtgt cggccatggg caggtccttc cggcaggtga cgatgattat gcacgcgcgc
      361 ggcgatacgg ctacattccg ccgcgatgga ggatgcgatg cgcacacagg ttggcattgt
      421 cggggccgga ccagccggcc tgttgttgtc gcatctgctg catttgcagg gtatcgagtc
      481 ggtcgtgctg gaggcacgga cgcgcgaata cgtggagaat cgcatccggg ccggcgtgct
      541 ggaatacggc accgagcaga tactgaacga ctctggcgta ggggatcgga tgcggcagga
      601 aggcatgctg catccgggcg ttgtgatgcg gtttgggcat gcggaccatc ggatcgattt
      661 cgctgatctg gttggcaagc acgtcatggt ctacagccag cacaaggtgg tgcaggacct
      721 gatcgctgcg cggctggccg gtggcggaca gatcgtgttc gaggctgacg atgtcagtgt
      781 gcatgacttc gaaggcgatg caccgcggat ccggtaccgc catgcgggcg atgtgcacga
      841 ggtggcgtgc gacatcatcg ccggttgcga cgggtttcat ggcgtttgcc gaccatccat
      901 tccggaccat ctgctgcgta tctacgagcg tgtctatccg ttcggctggc tgggaatcct
      961 ggcggagacg ccgccgtcca cggaggagaa tgtctatgtg agccacgagc gaggctttgc
     1021 gttgctggcg atgcgctcgc cgacgctgag ccggctctat ctgcaatgcg cgccggacga
     1081 ggacctggcg gcgtggtcgg atgatcggat ctggtcggaa ttgcagacgc gcgcggcgac
     1141 cgatgatttc cggctggcgg aagggccgat cctgcagcgc ggtatcacgc cgatgcggag
     1201 tttcgtgacc gagccgatgc agcgcggccg gctgtacctg gcgggcgacg cggcgcacat
     1261 tgtgccgccg acgggggcga aggggctgaa ccttgccgct gccgatgtgg cgatgctgac
     1321 gcgcgggatc gtggcgttct atcgcgacgg ccggaccgac atgctggacg agtattcagc
     1381 gacgtgtctg cggcggatct ggaaggtgca gcggttctcc tggtggatga cgacgctgct
     1441 gcatcggttc ttcgaccaca cgccgttcga gcagcagatg caggcggcgg agctggacta
     1501 tgtgttcagc tcgcgcgccg catcgctggc attggcggag aactacgtcg ggctgccgct
     1561 atagcccgcc ggtgacgtcg atggtctggc cggtgacgta gtcggcatcg gacgaggcga
     1621 ggaagcagat caccttcgcc tggtcggctg cctcagccat gcggcgcagg gggacgcgct
     1681 cgatttccgc ggcctggtct tcgggggatt gctggttgaa gcgggggcgg atgcgctcgg
     1741 tcagcgtgcg gctcggcgcg atggcgttga cgttgatgtt gtagggaccg agttcgagcg
     1801 agagcttgcg ggtaagcgcg atgatgccgc ccttggcggc ggcgtaggcg ccgctggagg
     1861 cgatgctgag gcctcggccg gcgatggagg cgagattgac gatcttgccg ctcttgcgcc
     1921 gcttcatcag cgggatgacg gcatggcaga acaggaatgt gccgtcgagg ttgaaggcga
     1981 ccagcctttg ccagtcgctg aggctgagtt cgtcgagtgt ggcacctgga ttgccgatga
     2041 tggtgctgcc gccgacggcg ttcaccagga tgtcgatcgt gccgtactgc ctggcagccc
     2101 aggcgacgac ttcggcgacc tgtgcttcgt ccagtgcgtt ggtggggcga ccgatggcgc
     2161 gaccaccgga catgttgatg gcctccattg ccatcgcgag gcggcgttcg tcgttgtcca
     2221 cgccgatgat gacggcgcct tccgcggcca tgatgtcggc ggtggctcgg ccgatgccgc
     2281 tgccgaccgc ggtgatgagg gctaccttgt ccttgaagcg cattttttgc ttcctccctt
     2341 ggcacgtcat tccatcgaca gtgacgagtc aaccgctgca cgtcccgcga tatcagcggc
     2401 gacgcgcgcc ggctggccgt cttctcgttt ctgccggtcg ctgcgggtca gttcgaccca
     2461 accggccagc agggtgctgg cgcgcgcggc aaaatccagc tgcagcacgc gggcgccgcg
     2521 gatccggtcg gtgcggaaat ggcgatagtc gtcgcgcagc tcgcaccagg cgcagatcag
     2581 ggtggcttcg acgtggtact ccatcgccac ggggcagatc gttcgggagg tggccgtgcc
     2641 tttctcatcc acgtagtcga tccacatttt gtgcgcattg cggatggcgg cgcgcgtctc
     2701 tgacagatcg atcgcgctgg gcgatggcgc gccacgcgtg gcgacatgga acggtgcctc
     2761 gagcaattgc gcgcgcaaga cctcgggcat cgctgccgcg agcttgacca gcacctgccg
     2821 cgcggcggag tccagtcccg ggtcaccagt ccggcgcagc agtcgcaggc cgacggcgac
     2881 cgcctgcacc tcttcctcgg tcagcatcag cggggggagt tcgaagccgc gccgcagcac
     2941 gtagccaacc cccgcttctc cgtcgatcgg cacccggcgc gactgcaatg tggcgatgtc
     3001 gcgatagacg gtgcgcggcg tcacctccag ctcggccgcg atagtcgccg cagtaatcgg
     3061 agtgcgcgcc ag
//
LOCUS       DATGEM010000568        10193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_489713, whole genome shotgun sequence.
ACCESSION   DATGEM010000568 DATGEM010000000
VERSION     DATGEM010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489713"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VKQ27_17335"
     CDS             <1..747
                     /locus_tag="VKQ27_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90749.1"
                     /translation="VVAEIKAAGGEAVANGADVSDFAQAKAMIDQAVQTFGKLDAVIN
                     NAGILRDKAFVNMTTEDFDFTVQVHFFGTVYCTKAAWPIMRKQQYGRIVVTTSGSGTV
                     GNFGQSNYGAAKMGVNGLINVLRFEGAKYNILLNAISPSAHTRMTEGLLPPDMVKFMR
                     PEFVSPAVAWLCSEECNASGEIIAATAGNYARVQYFQSEGVQFDPDQPVTLEMFAEKV
                     AQIRDLSKAKPYTGLMGTVEQSLRAMGKIS"
     gene            complement(829..1680)
                     /locus_tag="VKQ27_17340"
     CDS             complement(829..1680)
                     /locus_tag="VKQ27_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY90750.1"
                     /translation="MQWRIGDVTVTKVVELEVTGGSRFILPQATREAVQPIAWLVPHF
                     ADADGRLRMSIHSFVVQTPSRRIVVDTCLGNDKQGRRIPTWNDLHGPFLEDLAAAGYP
                     RESIDTVLCTHLHVDHVGWNTMLVDGRWVPTFPQARYLMGRVEYEHWSKPRGREDMVT
                     VFGDSVQPVFDAGLVDLVETDARLCEEIELVPTVGHTPGHVSVRIRSRGEEALITGDF
                     LHHPCQIAHPEWASTADSDPEMGIRTRERMFGELAGKPVLVIGTHFAGATAGRLVRDG
                     ATYRLDV"
     gene            1746..2348
                     /locus_tag="VKQ27_17345"
     CDS             1746..2348
                     /locus_tag="VKQ27_17345"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HLY90751.1"
                     /translation="MSHIDYYVSLNSPWTHLGAARIEALAAKYNASMRIHPVDFGTVF
                     AASGGLPLPKRAPQRQAYRLMELRRWKEFLGVPINIEPKHFPSSEALTAPCVIALRET
                     QGDALAIQLAHRVLKAVWQDEMNPGDPATLARLIGEIGLDADAVMKLGAEPRWTERRV
                     ADTKAALDRGVFGAPSYLINGETFWGQDRLDFVERKLANV"
     gene            complement(2460..3722)
                     /locus_tag="VKQ27_17350"
     CDS             complement(2460..3722)
                     /locus_tag="VKQ27_17350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HLY90752.1"
                     /translation="MRRVVLANETDWEGWRKATRSLVLAGVEPDEVRWAVRSHNEAGD
                     PLQDGTGSFGVSRSLVSLASVAIQAREKTRFDLLYRVVWNANAGQTEDELLRRAQGLA
                     LAARAEAHRMRTHIRFLPVDGVNVGWYEPAHFVLEANAQLIARRFPEQRFAIVTPDGA
                     AQWDGTELRFAAGARIEDDDALRDWRMPNDARVGTAIPEAEDLDEAPRPPDRPALGPV
                     VLRAHADRPLIEAMHEASDCRRCHLYEAATQTVFGEGPAHAKVLFVGEQPGDQEDVIG
                     RPFVGPAGQIMDRAMEEAGIDRRTIYITNAVKHFKFTPRGKRRIHQTPEAPEIQACRF
                     WLDVELVRMQPKLIVAMGGTAARALLGRPVTITRERGRAIELPDGQRAFVTVHPSFLL
                     RVPDEDAKTREYRAFVEDLRKVAELVDE"
     gene            3764..5491
                     /locus_tag="VKQ27_17355"
     CDS             3764..5491
                     /locus_tag="VKQ27_17355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLY90753.1"
                     /translation="MPYDPEHFVTIGAATSPSFSRDGTTLFHLRGSGLPQVWSLDLAS
                     GEERQLTFHDEKVAVLRRSPVDDRLIYGTDRGGDERQQLWLLEPGDEPHALTANTSVI
                     HDFGAWSPDATRIAYAANERDEAHFDVYVQDVATGVRQCVHQGDNIVTVSGFRPDGTT
                     LTLLHDRGYGDMSLLLLDLASGATAEAGHPANWQSVRWASDGESLLGLTDLDSEFLRL
                     CRLHGAITVVYAADGRDVEAWSLSPNGQMLATVENDRGYSVLRVGPLDGERPIVPDLP
                     RGVVSDLTWSPDSTRLAFSAASPTQPPGLWLWRDGSARALWQPETSSFVDLELVEWAS
                     FDGARIPGWLALPRAGKPPFPAIVWVHGGPVGQTRPNFRPDIQMLLAQGFAVLMPNVR
                     GSSGYGRSYTESDDVARRLDSVTDLAHGRAWLAAHPAIDAERIGIMGQSYGGFMVMSA
                     ITEHPDLWRAAVNYYGIADFTTLLEKTGSWRRNHRAAEYGDDAELFARISPIHRVDRI
                     QVPVLIAHGTRDPRVPFSESEQYVTALQERQKKVAFETFDYAGHGFIRPDDRRRVYRA
                     VAEFFATHL"
     gene            5545..7110
                     /gene="ggt"
                     /locus_tag="VKQ27_17360"
     CDS             5545..7110
                     /gene="ggt"
                     /locus_tag="VKQ27_17360"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLY90754.1"
                     /translation="MGRNGAVGANHPLATQAGLDTLRAGGNAVDAAVATSLALSVVEP
                     CMSGLGGDGFYQVYTAADGIARCYNATGSAPLAATPERFRARGIAVRGPLSVSTPGLV
                     GGLGAMHAAHGSLPWRQMFGPAIEHARDGFAASHHYRHFAEEVRAVLAADTRSGLVFP
                     PGPLGSLIRQPDLARTLEQIAQEGAETFYRGRLAKRLAAGMREAGVLVDERDLEAYQP
                     QIQDPIAIAYRGFRVTQTPPNSTGFTMLQMLKIAERFDIAALDPVRRIHVLVEAKKRA
                     FLDRERYGTDPRFGDVPLDRLLSDAYADECAAAIDMARAADMALAEPEEAVGDTTYFC
                     VVDAEGNAVSGIQSLNSGFGSGVTAGDTGVLLNNRMAYWHLAPGHANRLAPGKRVRHT
                     MNAPMVFKDDRLWVVEGTPGADNQVQVNLQVLTAMLDLGADPQTALEAPRWTSSQQGQ
                     GANWPHEGDGRLSIEPEFGADVLNGLEHLGHRLNRLASLDGPCSVQAIRIAGNGVRMA
                     GSDPRRDGWAGAY"
     gene            7118..8053
                     /locus_tag="VKQ27_17365"
     CDS             7118..8053
                     /locus_tag="VKQ27_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLY90755.1"
                     /translation="MRRFWNSAWILLILANLFWAANIVLGRGVVGMVPPIALAFWRWF
                     GAFLVAVPFAWPYLKRDLPTLLRHWPLMLVLSATGIATYNTMSYIGLTSTTALNVLLL
                     QSAGPLIIIIWAFLLFREWPSPRQVLAVLLSLAGVAVIAAHGSLEALLHLSLNAGDVW
                     ILAAMVIYGIYAAMFRVRPAVHPMSFLVATMGIGSMMILPFYLWEFAEGGRIQGSIPA
                     LLAMAYIAVFPSFIAYLFFNRGIELIGAARAGQSWHLMPVFGSILAVMFLGERFYAYH
                     AAGIALIAGGIVLASVKTGRQTPVARSPERLRSVQ"
     gene            8299..8706
                     /locus_tag="VKQ27_17370"
     CDS             8299..8706
                     /locus_tag="VKQ27_17370"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLY90756.1"
                     /translation="MRAAHRGTVTAVVALLLSAAGNAPPSNVQRVPPEQALAILGHTV
                     TGPDGKTIGRLVDVLVGDDGEPQAAVIDFGGFMGVGSRKIAVQWSTLHFAPANTKQPI
                     TLDLSQDQIKAAPEYKDPAKPAPVVMTPTTTSQ"
     gene            8710..9906
                     /locus_tag="VKQ27_17375"
     CDS             8710..9906
                     /locus_tag="VKQ27_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90757.1"
                     /translation="MPRDGTLPARGPRALDLLNFFVADVQTGFGPFVAVYLTAHKWTQ
                     VQIGFALTAGTIVGMLAQIPAGALVDATPNKRAAASGALIGVIIAALLLAVRPTQLPV
                     ILAQMLHAFASCVLTPAIAAISLHLVGHSALGERLGRNARFASIGNGLAAAVMGATGA
                     WLSSRAVFLLTAALCLPALIALAGIGRGPHSRRQTNARAMDWPGLRKLLSDRRLLVFG
                     ICVALYHLSNAAMLPLAGAEVTMRAGSYANIIIAGCILVPQGVVALLSPWVGRASAQI
                     GRRPLMLLGWAALPARGLLLAVLPGPWLLVAGQAVSGISAAVFGVMLPLLAADITRGS
                     SHFNLCMGLLGLAMYVGAALSTALGGWIADTVSLPVAFCALAAVGLAGTTVVWTMMPE
                     TRGASV"
     gene            complement(9898..>10193)
                     /locus_tag="VKQ27_17380"
     CDS             complement(9898..>10193)
                     /locus_tag="VKQ27_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in potassium efflux; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HLY90758.1"
                     /translation="LARARNRWFAHLLMDREVEGQVRDTYFSSLELARVALITLGVET
                     DAAARAVTVFREHDEKNLVETHAFYRDEQQMIQSGQQAAEELASLFEADRSSN"
BASE COUNT         1725 a         3636 c         3086 g         1746 t
ORIGIN      
        1 gtggtggccg agatcaaggc cgccggcggc gaggcggtgg ccaacggcgc cgacgtctcc
       61 gacttcgccc aggccaaggc catgatcgac caggcggtgc agaccttcgg caagctcgac
      121 gcggtgatca acaacgccgg catcctgcgc gacaaggcct tcgtcaacat gacgacggag
      181 gacttcgact tcaccgtgca ggtgcacttc ttcggcaccg tctactgcac caaggccgcc
      241 tggccgatca tgcgcaagca gcaatacggc cgcatcgtcg tcaccacgtc gggttccggc
      301 accgtcggca atttcggcca gagcaactac ggcgcagcga agatgggcgt gaacggcctc
      361 atcaacgtgc tgcgcttcga gggcgccaaa tacaacatcc tgctgaacgc gatctcgccc
      421 tccgcgcaca cgcgcatgac cgagggcctg ctgccgcccg acatggtgaa gttcatgcgt
      481 cccgagttcg tgtccccggc cgttgcctgg ctatgcagcg aggaatgcaa cgccagcggc
      541 gagatcatcg ccgcgaccgc cggtaactat gcccgcgtcc agtacttcca aagcgaaggc
      601 gtgcagttcg accccgacca gccggtgacg ctggagatgt tcgccgaaaa ggtggcgcag
      661 atccgcgatc tttcgaaggc gaagccttac accggcctta tgggcacggt agagcaaagc
      721 ctgcgcgcga tgggcaagat cagctaacgc cccgcttgtc attgcgagga gcgtagcgac
      781 gaagcaatct cgccaggatc gcttcgctcc tcgcattgac aaacgcccct acacgtccag
      841 ccggtatgtc gccccatcgc gcacgagcct gccagccgtc gcgccggcga agtgcgtgcc
      901 gatcaccagc accggcttgc ccgccagctc gccaaacatc cgctcccgcg tcctgatccc
      961 catctccgga tcggaatccg ccgtggaagc ccattccgga tgcgcgatct gacacggatg
     1021 gtgcaggaag tcgccggtga tcagcgcctc ctcgccgcgt gagcgaatgc gcacgctgac
     1081 atgaccaggc gtatgaccga ccgtgggcac aagctcgatc tcctcgcaga ggcgcgcatc
     1141 ggtctcgacc agatcaacca gtcccgcatc gaacacaggc tgcaccgaat cgccaaacac
     1201 cgtcaccata tcctcgcgcc cccgtggctt cgaccagtgc tcgtactcca cgcgccccat
     1261 cagatagcgc gcctgcggga acgtcggcac ccatctccca tccaccagca tcgtattcca
     1321 ccccacatga tccacgtgga gatgcgtgca cagcacggta tcgatcgact cgcgcgggta
     1381 accggccgcc gccagatcct ccaggaacgg cccatgcaga tcgttccatg tcggaatgcg
     1441 ccggccctgc ttgtcattcc caaggcacgt atccaccacg atccgccgtg acggcgtctg
     1501 cacgacgaag ctgtggatgc tcatgcgcag ccgcccgtca gcatcggcaa aatgcggcac
     1561 caaccacgca attggctgca ccgcctcgcg cgtcgcctgt ggcaggatga aacgggagcc
     1621 gccggtcacc tccagttcca cgaccttggt caccgtcacg tcgccaatcc gccactgcat
     1681 cgcgtcctcc ttgtgcgttg catgtcgccg tatcacgatt gacgctcccg cgggaggagc
     1741 gcgccgtgtc ccatatcgac tactacgtct cgctgaactc gccctggacc catctcggcg
     1801 cggcccggat cgaagctctg gcagcgaaat acaacgcaag catgcgcatc catccggtag
     1861 acttcggcac cgtattcgcc gcctccggcg gcctgccgtt gccgaagcgc gcgccgcaac
     1921 gccaagccta tcggctgatg gaattacgcc ggtggaagga attcctcggc gtgccgatca
     1981 acatcgaacc aaagcacttt ccctccagcg aggcgctcac cgcgccgtgc gtcatcgccc
     2041 tgcgggagac ccagggcgac gcactggcga tccagctcgc ccatcgagta ctgaaggccg
     2101 tgtggcagga tgagatgaac ccgggcgacc cagcgacgct ggcgcggctg attggcgaga
     2161 tcggactcga tgccgatgcc gtcatgaagc tcggcgccga accacgctgg accgagcggc
     2221 gggtggccga taccaaggcg gcgctcgacc gcggcgtatt cggcgcacca agctatctga
     2281 tcaacggcga gaccttctgg ggccaggatc gcctggactt cgtcgagcgg aagctggcta
     2341 acgtgtagca aatgtcattg cgaggagcgc agcggtggcc ggactcgacc cggccgagca
     2401 atctcgcggt gcgcggcatt aatcgcgcga ttgcttcttc gctgcgctcc tcgcattgtc
     2461 tattcgtcca ccaactccgc cactttcctc aaatcctcga caaacgcccg gtactcccgc
     2521 gtcttcgcat cttcgtccgg cacgcgcagc aggaaggacg gatgcaccgt cacgaacgca
     2581 cgttgcccat ccggcaactc gatcgcacgc ccccgctcac gggtaatcgt caccggccgc
     2641 ccaagcagcg cgcgtgccgc cgtgccaccc atcgcgacga tcagcttcgg ctgcatccgc
     2701 accagctcga catccagcca gaaccggcag gcctggatct ccggcgcctc cggcgtctgg
     2761 tggatacggc gcttcccgcg cggcgtgaac ttgaagtgct tcaccgcatt ggtgatgtag
     2821 atggtgcggc gatcgatgcc cgcttcctcc atcgcgcggt ccatgatctg tcccgctggg
     2881 ccaacgaacg ggcggccaat aacatcttcc tggtcgcctg gctgctcgcc cacgaacagc
     2941 accttcgcat gtgccggccc ctcaccgaac acagtctgcg tcgccgcctc atacaaatgg
     3001 cagcgacgac agtccgacgc ttcgtgcatc gcttcaatca gcggccgatc ggcgtgcgca
     3061 cgcagcacga cagggccgag cgccggccga tccggcgggc gaggcgcttc gtccaggtcc
     3121 tcggcttccg gaatggccgt cccgacccgc gcatcgttcg gcatccgcca atcgcgaaga
     3181 gcatcgtcat cctcgatccg tgccccggcc gcgaagcgca gttcagtccc atcccactgc
     3241 gccgccccgt caggcgtgac tatcgcgaac cgctgctccg gaaaccgccg cgcgatcaat
     3301 tgcgcgttgg cctccagcac gaaatgcgcc ggctcgtacc acccgacatt cacgccatcg
     3361 accggcaaga accggatgtg ggtgcgcatc cgatgcgcct ccgcgcgcgc cgccagcgca
     3421 agaccctgtg cgcgacgcaa cagttcgtcc tctgtctggc cggcgttggc gttccacaca
     3481 acccgataca gcaggtcgaa gcgcgtcttc tcgcgcgcct ggatggcgac cgacgccagc
     3541 gacaccagag agcgcgacac gccgaagctg cccgtgccat cctggagcgg atcgcccgct
     3601 tcattgtgtg agcggaccgc ccagcgcacc tcatcgggtt cgacgccggc gagcaccagg
     3661 gagcgtgtcg ccttgcgcca gccctcccaa tcggtctcat tcgccagtac aacccgtcgc
     3721 attgctggag caaaccctat cccgctaagg tttccaaccc accatgccct acgatcccga
     3781 gcacttcgtc accattggcg ccgcgacctc acccagcttc tcgcgtgacg gcacaacgct
     3841 cttccatctg cgtggttccg gcctgccgca ggtgtggtcg ctcgacctcg cgtccggcga
     3901 ggagcggcaa ctcaccttcc atgacgagaa ggtcgccgtg ctgcggcgct ccccggtcga
     3961 tgaccgcctg atctacggca cagatcgcgg cggcgatgag cgccagcagc tatggctgct
     4021 ggaaccaggc gacgaaccgc acgcgctcac cgccaatacg tcggtgatcc acgacttcgg
     4081 cgcctggtca cccgacgcca cgcgcatcgc ctatgccgca aacgaacgcg acgaggcgca
     4141 tttcgacgtc tatgtgcagg atgtcgcgac cggcgtacgc caatgcgtcc atcagggcga
     4201 caacatcgtc accgtgtccg gcttccggcc tgacgggaca accctgacgc tgctgcatga
     4261 ccgcggctat ggcgacatgt ccctgctgct gctggacctt gccagcggcg cgaccgccga
     4321 ggcaggtcac ccggcaaact ggcagagcgt gcgctgggcg agcgacggtg agtcgctgct
     4381 aggcctgacc gacctggaca gcgaattcct gcgcctgtgc cgcctgcatg gcgctataac
     4441 ggttgtatat gcagccgatg gccgcgacgt cgaggcgtgg tcgctgtcgc ccaatgggca
     4501 gatgctcgcc accgtggaga atgaccgcgg ctacagtgta ttgcgtgtcg gccccctgga
     4561 tggcgagcgg ccaatcgtgc cggacctccc gcgtggcgtc gtcagtgacc tgacatggtc
     4621 gcccgacagc actcgccttg cgttcagcgc cgcttcaccc acccagccgc caggattgtg
     4681 gctatggcgc gacggttcgg cacgcgcgct ctggcagccc gagaccagca gcttcgtcga
     4741 tctcgaactg gtcgaatggg ccagcttcga cggcgcgcgc atcccaggct ggctcgcgct
     4801 gccacgcgcc ggaaagccgc cgttcccggc gatagtctgg gtacatggcg gcccagtggg
     4861 acagacgagg ccaaacttcc gccccgacat ccagatgctg ctggcacagg gctttgccgt
     4921 gctgatgccg aacgtgcgcg gcagcagtgg ctacggccgc agctacaccg agagcgatga
     4981 tgtggccagg cgcctcgaca gcgtcaccga ccttgcgcac ggccgtgcct ggctcgcggc
     5041 gcatccggcg atcgacgccg aacgcatcgg catcatgggc cagtcctacg gcggcttcat
     5101 ggtgatgtcg gcgatcaccg agcatccgga cctctggcgc gcagcggtga actactatgg
     5161 catcgccgat ttcaccacgc tcctcgaaaa gaccggctcg tggcgccgca accatcgcgc
     5221 tgccgagtac ggcgacgatg ccgaactgtt cgcgcgcatt tcgccgatcc atcgggtgga
     5281 tcgcatccag gtccccgtgt tgatcgcgca cggcacgcgc gatcctcgcg tgccgttcag
     5341 cgaaagcgag caatacgtga ccgcgctcca ggagcggcag aagaaggttg cgttcgaaac
     5401 cttcgactat gccggccatg gcttcatccg ccccgacgat cggcgccgcg tctatcgcgc
     5461 ggtcgccgag ttcttcgcga cccacctgta gcgccaggac ccaaacgcat gtacctcccc
     5521 gcccgcaacc accgcgttct gatcatgggc cgcaacggcg cggtcggcgc caatcacccg
     5581 ctcgccacgc aagccggcct cgacaccctg cgcgccggcg gcaatgccgt tgacgccgcc
     5641 gtggcgacct cgctcgcgct gagcgtcgtg gagccatgca tgtccggtct cggcggcgac
     5701 ggcttctacc aggtctacac ggccgccgac ggcatcgccc gctgctacaa cgcgaccggc
     5761 agcgcacctc tggccgcgac gcccgagcgc ttccgcgcgc gcggcatcgc agtgcgcggc
     5821 ccactcagcg tttcgacgcc aggactggtg ggcggcctcg gcgcgatgca cgccgcgcac
     5881 ggaagcctgc cgtggcgcca gatgttcggc cccgcgatcg agcacgcccg cgacggcttc
     5941 gccgctagcc atcactaccg ccacttcgcc gaggaagtcc gcgccgtcct cgccgccgac
     6001 actcgctccg gcctggtctt ccctcccggc ccgctcggct cgttgatccg ccagcctgac
     6061 cttgcacgca ccctggaaca gatcgcccag gaaggcgccg agaccttcta ccgtggccgc
     6121 ctggcaaagc gcctcgccgc cggcatgcgc gaagctggtg tcctggtcga tgagcgcgac
     6181 ctcgaggcat accagccgca gatccaggac ccgatcgcca tcgcctatcg cggcttccga
     6241 gtgacccaga ccccgcccaa ttccaccggc ttcaccatgt tgcagatgct gaagatcgcc
     6301 gagcgcttcg acatcgcggc gctcgacccg gtccgtcgca tccacgtcct ggtcgaagcc
     6361 aagaagcgcg ccttcctcga tcgcgagcgc tacggcaccg atccaaggtt cggcgacgtg
     6421 ccgctcgatc gcctgctctc cgatgcctat gccgacgaat gcgccgctgc catcgatatg
     6481 gcgcgcgctg ccgatatggc gctggcggaa cccgaggaag ccgtcggcga caccacctat
     6541 ttctgcgtcg tcgatgccga gggcaatgcc gtctccggca tccagagcct caactccggc
     6601 ttcggctccg gcgtcaccgc cggggacacc ggcgtcctcc tcaacaaccg catggcctac
     6661 tggcatctcg cccccggcca cgccaaccgc ctcgcacctg gcaaacgcgt gcgccacacc
     6721 atgaatgcgc cgatggtgtt caaggacgac cggctgtggg tcgtcgaagg cacacccggc
     6781 gcagacaacc aggtccaggt gaacctccag gtgctgaccg cgatgttgga cctgggtgcg
     6841 gacccgcaga ccgctctgga agcaccgcgc tggacctcca gccagcaggg ccagggcgcg
     6901 aactggccgc atgaaggcga tggccgcctc tccatcgagc cggagttcgg cgcggacgtg
     6961 ctgaatggat tggaacacct gggccaccgc ctcaaccggc tggcctcgtt ggacggccca
     7021 tgcagtgtgc aggccatccg catcgccggg aacggcgttc gcatggccgg ctccgatcca
     7081 cgccgcgacg gctgggccgg cgcctactga tccacccatg cgccgtttct ggaattccgc
     7141 ctggatcctg ttgatcctgg ccaacctctt ctgggccgcg aacatcgtcc tggggcgtgg
     7201 cgtcgtcggc atggtaccgc cgatcgcgct ggcattctgg cggtggttcg gcgctttcct
     7261 cgtcgccgtc ccgttcgcat ggccctacct gaagcgggac ctgccgacgc tgctccgaca
     7321 ctggcccctg atgctcgtgc tctccgcgac cggcatcgcg acctacaaca ccatgtccta
     7381 tatcggcctg acctccacga cggcgctgaa cgtgctgctg ctgcagtcag cgggaccgct
     7441 gatcatcatc atctgggcct tcctcctgtt ccgggaatgg ccatcgccac ggcaggtcct
     7501 cgccgtgctc ctgtcgttgg ccggcgtggc cgtgatcgcc gcgcacggtt cgctggaagc
     7561 gctgctgcat ctgtcgctga acgccggcga cgtgtggatc ctcgcggcaa tggtgatcta
     7621 tggcatctac gctgccatgt tccgcgtacg gcctgcggtg catcccatga gcttcctggt
     7681 cgccacgatg ggcatcggct cgatgatgat cctgccattc tacctgtggg aatttgccga
     7741 aggcgggcgc atccagggta gcattccggc gctgctggca atggcgtaca tcgccgtgtt
     7801 cccctcgttc atcgcctacc tgttcttcaa ccgcggcatc gagctgatcg gcgcggcgag
     7861 ggccggccaa tcgtggcacc tgatgcccgt attcggttcg atccttgccg tgatgttcct
     7921 cggcgagcgc ttctacgcct atcacgcggc cggcatcgcg ctgatcgcag gaggcattgt
     7981 actcgcatcc gtgaagacag ggcggcagac gccggtcgct cgttctccgg aacggcttag
     8041 atcggtacaa tgaaaattga gcagcatcct tccccatctc taacggttgc gtgcgccacg
     8101 cattggtgat tcgcgggcag cgcttcatcg gcgcaatgat gcggagcgac tacaatcccc
     8161 atactggcgt gcgatcgcac cttgatcgca cgccgcattt tgggtcgtgc tcgacgtcgc
     8221 cagcaccgca cccaaacttg aagtccgcct ctccgccgcg cgcttgaccg cgtgccgttt
     8281 cgctccgacc tgccgcacat gcgtgcagcg caccggggaa cagtcacggc agtcgtcgcg
     8341 ttgctgctat cggcggcggg aaacgcgccg cccagcaacg tacagcgcgt cccgccggag
     8401 caggcattgg caatcctcgg ccacactgtg acaggtccag acggcaagac catcggccgc
     8461 ctcgtcgacg tcctggtggg cgacgacggg gaaccgcagg ccgcggtgat cgacttcggc
     8521 ggcttcatgg gcgtgggcag ccgcaagatc gcggtgcagt ggagcacgct tcacttcgcc
     8581 cccgcgaaca cgaagcagcc aatcacgctc gacctgtccc aggaccagat caaggctgca
     8641 ccggaataca aggacccggc aaaaccagcg cccgtggtga tgacgcccac aaccacgtcg
     8701 caatgacgca tgccacgcga tggcacactt ccggcgcgag ggccacgggc actcgatctg
     8761 ctgaatttct tcgttgccga tgtgcagacc ggcttcggtc cgttcgtcgc cgtctatctc
     8821 accgcacaca aatggacgca ggtgcagatt ggctttgccc tgactgcggg aaccatcgtc
     8881 gggatgctcg cgcagatccc cgctggcgcc ctggttgatg caacgcccaa caagcgagca
     8941 gcagcaagcg gcgcactcat cggtgtcatc atcgcggcac tgctgcttgc agtgcggccg
     9001 acgcagttgc cggtgatcct cgcacagatg ctgcacgcct tcgccagctg cgtgcttacg
     9061 ccggccatcg cggcaatcag cctgcatctg gtcgggcatt ccgcgctcgg tgagcgattg
     9121 ggccgcaatg cccgctttgc ctcgatcggg aatggcctgg cagcggcggt gatgggagca
     9181 accggcgcgt ggctgtcgag ccgtgcggta ttcctgctga ccgcagcatt gtgcctgccc
     9241 gccctcatcg cgctcgccgg catcggtcgc ggcccccact cccgccgtca aaccaatgca
     9301 cgcgcaatgg actggcccgg actgcgcaaa ctcctttccg atcgccgatt gctggtgttc
     9361 ggcatctgcg tcgcgctgta ccacttgtcg aatgcggcga tgctgcccct ggccggcgcc
     9421 gaagtgacga tgcgcgccgg ctcctatgcc aacataatca tcgccggctg catcctggtg
     9481 ccgcagggcg tcgtcgcact actgtcgcca tgggtcggac gtgcgtcggc acaaattgga
     9541 cgacggccgt tgatgctgct cggctgggca gccctgccag cacgtggact gctgctggcg
     9601 gtgctgccag gcccttggct cctggtcgcg ggccaggcgg tcagcggtat cagcgccgca
     9661 gtgtttggcg tcatgctgcc gctgctcgcc gccgacatca cgcgcggttc gtcgcacttc
     9721 aatctgtgca tgggattgct gggcctcgcg atgtacgtcg gtgcagctct cagcaccgcg
     9781 ctgggtggct ggatcgccga cacggtcagc ctccccgtgg ccttctgcgc gctcgcagcc
     9841 gtggggctcg cgggcacgac ggttgtctgg accatgatgc cggaaacccg cggcgcatca
     9901 gtttgacgag cggtcagcct cgaacaggct ggcgagttct tccgccgcct gctgcccgct
     9961 ttggatcatc tgctgctcat cgcggtaaaa cgcgtgggtc tccaccagat ttttctcatc
    10021 gtgttcgcgg aataccgtta ccgcgcgcgc cgcggcatcg gtctcgacgc cgagcgtgat
    10081 caacgcgacg cgcgccagct ccaggctgga aaagtacgtg tcgcgcacct ggccctcgac
    10141 ttcgcggtcc atcagcaaat gagcgaacca acgattgcgt gcccgcgcga gga
//
LOCUS       DATGEM010000569         8933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_949636, whole genome shotgun sequence.
ACCESSION   DATGEM010000569 DATGEM010000000
VERSION     DATGEM010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949636"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1203
                     /locus_tag="VKQ27_17385"
     CDS             13..1203
                     /locus_tag="VKQ27_17385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90759.1"
                     /translation="MPSTDASALPDTQRRWSLAAAITSVTVFGLSIGQAVPVMSLLLE
                     ERGTDASLNGLNAGSAFLGVMIGPLLAPRLIRWFGIRNFLIAGFVIDIALFLAMKPLD
                     SMGAWFVLRALLGLVGSAIFTSSEAWINQLAGDIGRGRIIGIYAAALSAGFGIGPLLL
                     AITGINGWAPFVANGAITALAIVPLLGVGDASRSFGRERGGSPLVMFARAPLIVGVVA
                     IFGLYEAALMALLPIWGVRSGLDNRMAAAALTAVYGGSILMQMLVGWLSDAVSRVAAL
                     RLCGVVGMAGALTLALFPQPWALVFGVLFLWGGIAAGIYPVALSMAGDRFRGADLVAV
                     NAAIIMAYGLGALVGPALGGVAMDIRDPQGLLWLFVAAFAVLLAATARQAVTAGPRLP
                     HGTG"
     gene            complement(1166..2059)
                     /locus_tag="VKQ27_17390"
     CDS             complement(1166..2059)
                     /locus_tag="VKQ27_17390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HLY90760.1"
                     /translation="MQLGASLPVADIGTGPAVVRDYAQAAEGLGYDYLVAPDHVLGAN
                     PAADHGGRRVGTTASAYHDPFVLFGFLAGCTQRIGFAVGVLILAQRQAVLVAKQAASL
                     DVLCGGRFRLGIGVGWNEVEFLGLNENFHNRGKRSAEQVRVMQALWANEHVKFEGEFH
                     RIEDAGINPRPPSGRVPIWYGGHAEATFRRAAKFGDGFMPLAYPAGDAALAAFDKLRA
                     LTREAGRDPAKMGIEVWASLAGTEDDWRREFRFWKQAGVTHVTAHTTYTSNHHKRIDG
                     RSAEAHLAAITRYRAAVADLL"
     gene            complement(2091..3065)
                     /locus_tag="VKQ27_17395"
     CDS             complement(2091..3065)
                     /locus_tag="VKQ27_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90761.1"
                     /translation="MPGQKKRAVMVLGMHRSGTSAVAGAVDLLGAERPASMLAGGPGN
                     PLGYFESRGVIHVNDWICRMAGSTWYDSLPFDANSMDPISRNTAMALINIAIRAEFGD
                     ASLLLLKDPRLCLLLDYWLPMLDAMHIEPQVLLVLRHPAEVMASLNKRDAFPWPLAAA
                     AWLQHVLAAEHATRGRCRTLVIYDDLMTDWRGCLMRASQRAEITWPTTFDAVANQMAE
                     RLPRDLWHHRGGVMNAKVPAALVSLMQAAFDTLLDLSAGEPPAALRHLDEVRGAFDAW
                     RTTHGLPLAKALLDGHVLRRLPKEVVPEEWMQLAEGLAARVRKPQNSR"
     gene            3143..3607
                     /locus_tag="VKQ27_17400"
     CDS             3143..3607
                     /locus_tag="VKQ27_17400"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY90762.1"
                     /translation="MIVSDPGKAFGHCQCGGVVIEIGYPAFWAWHDHSPASRLAHGAA
                     YATYVGSWRKKFRVARGAGLIARYEDKAAGTTRAFCKRCGTPLYYERKRSRHMVNVPR
                     ALFAGRTGRHPLYHAHIEEMPDWAYAGGNLAPLKGYPGYVRERAPRRKTPPR"
     gene            3662..4918
                     /locus_tag="VKQ27_17405"
     CDS             3662..4918
                     /locus_tag="VKQ27_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90763.1"
                     /translation="MPLPADTDATTPKYLRDKYAIVGVGETTYTRGSGRTTRAMGTFA
                     VRAAMLDAGLKPSDIDGMLSYSGNDSTPSTFLAGDLGVRLNFFMDVNGGGSSTEALIG
                     IAIGVIEAGLCRCVAIFRSMNGYSQVRIGGTGARSAAPVNGDALHSRAYGWQSAGQMF
                     APTFMRHMYEYGTKPEQVAMVKVVHSEHASNNPKAFYKKRYTVDDVINSRIICKPLHL
                     LDCCVETDNATCIIITRAEQARDLRHTPALIRGVVGRCSKPRTDMHYQHGPISRVAGY
                     YAKDILWPNSGVGPEDIDAVGAYDAFTFTSMLQLEDYGFCKKGEGGQYVSSGITRLGG
                     RRPQNTSGGHLCEGYTHGMNMVIENVRQLRHDVDDYCPIGPDGKRQHTYDYSEGCCRQ
                     VKNIEVTANLGWANPMTGSAMVMRRG"
     gene            4934..5455
                     /locus_tag="VKQ27_17410"
     CDS             4934..5455
                     /locus_tag="VKQ27_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HLY90764.1"
                     /translation="MSVRGTYLDMPVSIDDLDGENLEYFRHCSEHKFHLQQCDDCKLL
                     RYPPTTACPWCTCLTSKWVPVEGKGTVYSYEEVHHGIQPAFQARTPYMVLLVELDTQK
                     AAPTEHESLRVAANLTTPDGILAPPDVVKRVGIGSRMRMVFSDVCPGLSLPQWTPDET
                     TAQPQKPWRYPQE"
     gene            complement(5530..6168)
                     /locus_tag="VKQ27_17415"
     CDS             complement(5530..6168)
                     /locus_tag="VKQ27_17415"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLY90765.1"
                     /translation="MSFLSFHHVLIQLLHEYGNVVLGIVVGLECVGLPLPGETLLIAA
                     GIIAGTTQHLNIFMVVISAALGAIVGQLGGYWIGWGIGFRLLRRYGRYIGLNDRRMAY
                     GRALFRKHGEKVVVVSRFVVLLRTLAGLLAGANHMAFGRFLIANVAGSVAWSALYGVG
                     AYMLGHAAKDVAGPVAIGFSALLLLGLGGTMLYVRRREHRLFSAPMRARVAK"
     gene            6248..8122
                     /gene="mnmG"
                     /locus_tag="VKQ27_17420"
     CDS             6248..8122
                     /gene="mnmG"
                     /locus_tag="VKQ27_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HLY90766.1"
                     /translation="MGEQFDVVVVGGGHAGAEAAAAAARVGARTLLLTHRIETIGEMS
                     CNPSIGGIGKGHLVREVDALDGLMGRAADMAGIHFKLLNRSKGPAVRGPRAQADRKLY
                     RNAMQQLLAEMPGLTLRAGAVEDLEIGPDGHLAAVTCADGSRIACGTAVLIAGTFLRG
                     VIHIGEEQRPAGRVGEAPAIGLARTLARLGLPLGRLKTGTPPRLDRRSIDWEGLDRDP
                     GDAEPEPFSTLTERITNPQIECRITGTTPATHAIIRTNLHRSAVYGGRIEGVGPRYCP
                     SIEDKIVRFAGRDRHNIFLEPEGLDDDTVYPNGISTSLPAEVQLQILATIPGLERARM
                     VRPGYAVEYDYVDPRSLTQGLEVRRLPGLFLAGQINGTTGYEEAAAQGLLAGVNAARL
                     AVGCGPATLDRSDAYIGVLVDDLTTQGVSEPYRMFTSRAEFRLTLRADNADMRLTAKG
                     QAWGCVGTTRAVAFAAHAAAVKLAMERARAEGGHPTELARRGLAVRADGRWRSVCDLL
                     SYPDIDFDAVASAFPWLRDLRPSALAQLQTEVRYEGYLSRQQADIRSFQRDEAISLTG
                     VAFSSIGGLSAELTAKLNHLQPGSLGAASRIQGMTPAALAAIAAHVRKHRGDGVSRGT
                     "
     gene            8112..8714
                     /gene="rsmG"
                     /locus_tag="VKQ27_17425"
     CDS             8112..8714
                     /gene="rsmG"
                     /locus_tag="VKQ27_17425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HLY90767.1"
                     /translation="MEHSSEPNDVPRETLDRFATLLLRWNRTVNLISANDEPLLWDRH
                     IGDSLQLVPLLVPPPDRAIDLGSGAGFPGLILAIATGIPFDLIEADKRKAAFLREAAR
                     ITGAPVQVHTVRIEAARVAPAPLVTARALAPLPRLLSLAAPLLTPDGTCLFLKGTNAR
                     TELTHAATQWHMQVQSVPSRTIPDACILRISHLSRVIPEP"
     gene            8695..>8933
                     /locus_tag="VKQ27_17430"
     CDS             8695..>8933
                     /locus_tag="VKQ27_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY90768.1"
                     /translation="MSSQNPDATRVLAVANQKGGVGKTTTAINLATALAANNEQVLLI
                     DLDPQGNASTGLGVPRTERATGSYRLLMGDATAKDA"
BASE COUNT         1598 a         2861 c         2880 g         1594 t
ORIGIN      
        1 taaggtcgat cgatgccaag cacagatgca agcgcgctgc cagacacgca gcggcgatgg
       61 agccttgctg ccgcgatcac atcggtgacg gtgttcggcc tgagcattgg tcaggccgtg
      121 cctgtaatgt cgctcctgtt ggaggaacga ggcaccgatg catcgctgaa tggcctgaat
      181 gccggttcgg cgttccttgg tgtgatgatc ggtccgctgc ttgcgccgcg cctgatccgc
      241 tggttcggca tccgcaactt cctgatcgct ggcttcgtca tcgatatcgc gctgttcctg
      301 gcgatgaagc cgctcgacag catgggcgcc tggttcgtgc tgcgagcgct gcttgggctg
      361 gtcggttcgg cgatcttcac gtccagcgag gcgtggatca accagctcgc aggcgacatc
      421 gggcggggcc gtatcatcgg catctacgcc gccgcgctct ccgcgggctt tggcatcggg
      481 ccgctgcttc tggcgatcac cggcatcaac ggttgggcac ctttcgtggc caatggtgcg
      541 atcaccgcac tggcgattgt gccgctgctc ggggttggcg atgcatcgcg ctcatttggc
      601 cgcgaacgcg gcggcagtcc gttggtgatg tttgcgcgcg caccgctgat cgtcggtgtg
      661 gtcgcgatat tcgggctgta cgaggccgcg ctgatggcgc tgctgccgat ctggggcgtg
      721 cgcagcgggc tggacaatcg catggcagcg gctgcgctta ccgcggtcta tggtggctcg
      781 atcctgatgc agatgctggt gggctggctc tccgacgcag tgtcccgcgt ggccgcgttg
      841 cgactgtgcg gggtggtcgg gatggcgggg gcactgacac tggcgttgtt cccccagcca
      901 tgggctctgg tgtttggcgt gctgttcctt tggggcggta ttgccgcagg catctatccc
      961 gtggcgctca gtatggctgg cgaccggttt cgaggtgcgg atctggtagc ggtcaacgcg
     1021 gcgatcatca tggcatatgg cctgggcgcg ctggttggcc cagcgctcgg cggcgtggcg
     1081 atggacatcc gcgatccgca ggggctgcta tggctgttcg ttgcggcttt cgcggtgctg
     1141 ctggcagcca cagcccggca ggcggttaca gcaggtccgc gactgccgca cggtaccggg
     1201 tgatcgccgc cagatgcgcc tcggcgctgc gtccgtcgat acgtttgtgg tgattgctgg
     1261 tataggtcgt gtgcgccgtc acatgcgtca cgccggcctg cttccagaac cggaactcgc
     1321 gccgccagtc atcctcggtg ccggccagcg acgcccatac ctcgatgccc attttcgccg
     1381 ggtcgcgccc tgcctcgcgc gtcagcgcgc gcagcttgtc gaacgccgcc agcgccgcat
     1441 caccggccgg ataggccagc ggcatgaagc cgtcgccaaa cttcgccgcg cggcggaagg
     1501 tcgcctcggc gtggccgcca taccatatcg gcacacgccc tgatggcgga cgcggattga
     1561 tgccggcgtc ctcgatgcga tggaactcgc cctcgaactt cacgtgctcg ttcgcccaca
     1621 gcgcctgcat cacccgcacc tgttcagccg accgcttgcc gcggttgtgg aaattctcgt
     1681 tcagaccgag gaattcgacc tcgttccagc caacgccgat gcccaggcgg aaccgcccgc
     1741 cacacagcac gtcgagcgag gcggcctgct ttgccaccaa caccgcctgg cgctgcgcca
     1801 ggatcagcac gccgaccgca aagccgatac gctgtgtgca gccggccagg aagccgaaca
     1861 gcacgaatgg atcgtgatac gcactggcag tcgtgccgac acgccggccg ccgtgatcgg
     1921 ccgccggatt ggcacccagc acgtggtccg gtgcgaccag gtagtcgtag ccaagtccct
     1981 cagccgcctg cgcataatca cgcacgacgg ccggaccggt gccaatgtcc gcgaccggaa
     2041 gcgaggcgcc aagctgcatg atcgttctcc cctttgttga tgggagcacg ctagcgcgaa
     2101 ttctggggtt ttctcacgcg ggccgccagg ccttcggcga gctgcatcca ttcttcagga
     2161 acgacctcct tcggcaggcg gcgcagcacg tggccgtcca gcaatgcctt cgccagcggc
     2221 aaaccgtgcg tggtgcgcca tgcgtcaaag gcgccgcgca cctcatcgag gtgtcgcaat
     2281 gccgccggcg gttcaccggc gctcagatcg agcagcgtat cgaacgcggc ctgcatcagt
     2341 gacaccaggg cagccggcac cttggcattc atcacaccgc cgcgatgatg ccatagatca
     2401 cgcggcaggc gttctgccat ctggttcgcc accgcgtcga acgtcgtcgg ccaggtgatc
     2461 tctgcgcgct ggcttgcacg catcaggcag ccgcgccagt cagtcatcag gtcgtcgtaa
     2521 atcaccagcg tccgacaacg gccgcgcgtc gcatgctcgg cggccagcac gtgctgcaac
     2581 caggcggcgg cggccagcgg ccatgggaac gcatcacgct tgttcagcga cgccatcacc
     2641 tcggccggat ggcggagcac gagcaacacc tgaggttcga tgtgcatcgc atccagcatt
     2701 ggcagccaat agtcgagcag caagcaaagc cggggatcct tcagcagcaa cagagaggca
     2761 tcgccgaact cggccctgat cgcgatgttg atcagggcca tcgccgtgtt gcgggagatg
     2821 ggatccattg agttcgcgtc gaagggaaga ctgtcgtacc aggtgctgcc cgccatgcgg
     2881 cagatccagt cgttgacatg gatgacgcca cgcgattcga aatagcctag cgggttgccc
     2941 gggccgcccg cgagcatgga tgccggtcgt tcggcgccaa gcaggtccac cgcgccggca
     3001 acggccgaag tgccgctgcg gtgcatgccg agaaccatga ctgctcgttt cttctgaccc
     3061 ggcatggcgt gctgctgctg agcatcggga atgactgatc gtacgattgc cccaacagct
     3121 taatggaaag taaacgtctg acatgatcgt atccgatccg ggaaaggcct tcggccattg
     3181 ccaatgcggc ggtgtcgtca tcgagatcgg gtatcctgcg ttctgggcat ggcacgacca
     3241 ctcgccggcg agccgcctcg cacatggcgc cgcctacgcg acttacgtcg ggagctggcg
     3301 caagaaattc cgcgtcgcgc ggggggccgg cctgatcgcc cgttatgagg acaaggctgc
     3361 tggtacaacg cgcgcctttt gcaagcgctg tggcacgcca ctctattacg agcggaaacg
     3421 ctcgcggcac atggtgaatg tcccgcgtgc gttgttcgcg ggccgcaccg gacgccatcc
     3481 gctgtatcac gcgcatatcg aggagatgcc ggactgggcc tatgccggcg ggaacctggc
     3541 gccgctgaaa ggctatcccg gctacgtccg agaacgtgcg ccgcgccgga agacgccccc
     3601 gcgttgacgc ctccacgcat acgggtcagc ctaccggcca gtagcaaagg gggaaacgca
     3661 catgccgcta ccggcagaca ccgatgccac cacacccaag tatctgcgcg acaagtacgc
     3721 gatcgtcggc gtcggcgaga cgacgtatac acgcggctcc ggccgcacga cgcgtgccat
     3781 gggcaccttc gcggtgcgcg cggcgatgct ggatgccgga ctgaagccgt ccgacatcga
     3841 cggcatgctg tcctattccg gcaatgattc cacgccgtcc acgttcctgg ccggcgacct
     3901 cggcgtccgg ctgaacttct tcatggacgt gaatggcggc ggctcctcga ccgaggcgct
     3961 catcggcatc gcgatcggcg tgatcgaagc cggcctgtgc aggtgcgtgg ctatcttccg
     4021 ctcaatgaac ggctattcgc aggtacgtat cggcggcacg ggcgcgcgtt cagcagcacc
     4081 ggtgaatggc gatgcgctgc acagccgcgc ttacggctgg cagagcgccg ggcagatgtt
     4141 cgcgccgacg ttcatgcgcc acatgtacga gtacggcaca aagccagagc aggtggcaat
     4201 ggtgaaggtg gtgcactccg agcacgcctc caacaatccg aaggcgttct acaagaagcg
     4261 ctacacagtg gatgacgtga tcaacagccg catcatctgc aagccgctgc acctgctgga
     4321 ctgctgcgtc gagaccgaca acgccacctg catcatcatc acccgggccg aacaggcacg
     4381 cgatctgcgc catacgccgg cgctgatccg cggcgtggtg ggccgatgct cgaagccgcg
     4441 caccgacatg cactatcagc acgggccgat ctcacgcgtg gccggctatt acgccaagga
     4501 tatcctgtgg ccgaattccg gtgtcgggcc ggaggatatc gacgcggtcg gcgcgtatga
     4561 cgcgttcacc ttcacctcga tgctacagct cgaggactat ggtttctgca agaagggcga
     4621 gggcggccag tacgtatcgt cgggcatcac aaggctcggc ggcaggcggc cacagaacac
     4681 ctcgggcggc catctgtgcg aaggttacac gcacgggatg aacatggtga tcgagaacgt
     4741 gcggcagctg cgccacgacg tcgacgatta ctgtccgatc ggtcccgatg gcaagcgtca
     4801 acacacgtat gactacagcg aaggctgctg ccgccaggtg aagaacatcg aagtgactgc
     4861 caatctcggc tgggccaatc cgatgacggg ttcggccatg gtcatgcggc gtggctgagg
     4921 ggaggaatcg atcatgtcag ttcgtggcac atatctcgac atgccggtgt cgatcgacga
     4981 tctggacggc gagaacctgg agtatttccg ccattgctcc gagcataaat tccatctgca
     5041 gcaatgcgac gattgcaagc tactgcgcta tccaccgacc accgcttgtc cctggtgcac
     5101 atgcctgacg tcgaagtggg tgccggtgga agggaagggc acggtctact cgtatgagga
     5161 agtgcaccat ggcatccagc cggccttcca ggcgcgtaca ccttacatgg tgctgctggt
     5221 ggaactggac acacagaagg cggcaccaac ggaacatgag tcgctgcgcg tggccgccaa
     5281 cctgacgaca cctgacggta tcctggcgcc gccagatgtg gtgaagcgtg tcggcattgg
     5341 ctcgcgcatg cggatggtgt tcagcgatgt atgtccgggc ctgtcgctgc cgcaatggac
     5401 cccggacgaa acgacagcac agccacagaa gccttggcgc tatccgcaag aataagtgaa
     5461 gtatcgataa gttgtcatgg ccgggtatta cctggtcatg gcaatcactg gagcatcggc
     5521 actgtgccgt tatttcgcca cgcgtgcgcg cattggcgcc gagaacagcc gatgttcgcg
     5581 ccggcgcacg tacagcatgg tgcccccaag ccccagcaag agcagtgcgc tgaagccgat
     5641 tgctaccgga cctgcaacgt ccttcgccgc gtgccccagc atataggcgc cgacaccata
     5701 tagcgccgac cacgcgacgc tgcccgcgac attcgcgatc aggaaccggc cgaatgccat
     5761 gtggttggcg cccgccagca ggccagccaa tgtgcgcaac agcacgacga agcgcgatac
     5821 caccacgact ttctcgccgt gtttgcgaaa caatgcgcgg ccataggcca tccggcgatc
     5881 attcagcccg atatagcggc catagcgccg gagcaggcgg aacccgatgc cccaaccgat
     5941 ccaatagccg cccagctgcc cgacaattgc gcccaaggcg gcggatatca cgaccatgaa
     6001 aatattcagg tgctgcgtcg ttcctgcgat gatgcccgcg gcgatcagca gcgtttcgcc
     6061 aggcagcggc agccccacac attcgaggcc gacaacaatg ccaaggacga cgttgccata
     6121 ctcgtgcagc agttggatca ggacgtgatg gaaggataaa aaggacatga gatgtgcaat
     6181 gcggtgagta aatgccgtgg gtcaagtgtg tgcgtgacat ctcactgtaa caaggtattc
     6241 ggtcccaatg ggtgaacaat tcgacgtggt ggtggtgggt ggcgggcatg ctggcgccga
     6301 agcggcggca gcggccgcac gcgtcggcgc tcgtactctg ctgctgaccc acaggatcga
     6361 aacgatcggt gagatgtctt gcaatccctc gatcgggggc atcggcaagg gccacctcgt
     6421 gcgtgaggtt gatgcgctgg atggattgat gggccgcgcc gctgacatgg ccggcatcca
     6481 cttcaagctg ctgaatcgca gcaaggggcc tgccgtgcgc gggcctcgcg cccaggccga
     6541 ccgcaagctg tatcgcaacg cgatgcagca actgctggct gaaatgccgg ggctgacatt
     6601 gcgcgccggc gcggtcgagg atctggagat tgggccggac ggtcacttgg cggctgtgac
     6661 atgcgccgat ggcagccgca ttgcgtgcgg gacggccgtg ctgatcgccg ggacgttcct
     6721 gcgcggcgtg atccatatcg gcgaagagca acggccagca ggaagggtag gggaggcgcc
     6781 cgcgatcggc ctggctcgca cgctggcgcg gctagggttg ccgcttggtc ggctgaagac
     6841 ggggacgcct ccgcgcctcg accggcgcag catcgactgg gaggggctgg accgtgatcc
     6901 gggcgatgcg gagccggagc cgttcagcac gctcaccgag cgcatcacca acccgcagat
     6961 cgaatgccgc atcaccggga ccacgccggc gactcacgcc atcatccgca ccaacctgca
     7021 ccgttccgcg gtctatggcg gccggataga aggtgttggg ccgcgatatt gtccgtccat
     7081 cgaagacaag atcgtgcggt tcgcaggccg cgatcgccac aatatcttcc ttgagcccga
     7141 gggactggac gacgacacgg tctaccccaa cgggatatcg accagcctgc cggcagaggt
     7201 tcagctgcaa atcctggcga ccatccccgg cctggagcgt gcgcggatgg tccggcctgg
     7261 gtacgctgtc gaatacgatt acgtggatcc gcgatcgctt acccaggggc tggaagtccg
     7321 ccggttgccg gggctgttcc tcgcggggca gatcaacggc accacggggt atgaagaagc
     7381 tgccgctcag ggtttgttgg cgggcgtgaa cgctgctcgc ctagcggttg gctgcgggcc
     7441 tgccacactg gatcgttctg atgcctatat cggcgtgttg gtcgacgatc tgaccacgca
     7501 gggtgtcagc gagccatacc ggatgtttac ttcgcgggcg gaattccgtc tcactttgcg
     7561 agccgacaat gcagatatgc ggttgaccgc gaagggccag gcctggggtt gcgtcggcac
     7621 cacccgcgcc gtggcattcg ctgcgcatgc cgcggcagtg aagctcgcaa tggagcgtgc
     7681 ccgggcggag ggcggccatc cgacggagct tgcccggcgc gggctggctg tgcgtgctga
     7741 cggccgttgg cgctccgttt gcgacctgct gtcctatccg gacatcgatt ttgacgccgt
     7801 tgcttccgca ttcccgtggc tgcgggacct tcgtcccagt gccctggcgc aattgcagac
     7861 ggaagtgcgt tacgaaggct atctgtcacg ccagcaggcg gacattcgct ccttccagcg
     7921 ggacgaagcg atctcgctga ccggcgtcgc cttttcgtcg attgggggtc tgtcggccga
     7981 actcacagcc aagctcaatc acctgcagcc cggctcgctc ggcgcagctt cccggattca
     8041 agggatgacc ccggccgcac tcgccgcgat cgcggcccac gttcgcaagc accggggcga
     8101 cggcgtttca cgtggaacat agttcggagc cgaacgacgt tccacgtgaa acgctggatc
     8161 gtttcgccac cctgcttctt cggtggaacc gcacggtcaa cctgatctct gccaacgacg
     8221 agcccttgct ctgggatcgg cacattggcg attcgctgca actcgtcccc ttgctggtcc
     8281 ccccaccgga tcgcgcgatc gatctcggtt cgggtgcagg ttttcctggc ctgatcctgg
     8341 caattgccac cggcatcccc ttcgacctga ttgaggccga caagcgcaaa gccgcattcc
     8401 tgcgcgaggc ggcacggatc accggggcgc ccgtccaggt ccatacggtc cgcatcgagg
     8461 cggcgcgggt tgccccagca cccctggtca cggcccgggc gcttgctccg cttcccaggc
     8521 tcctctccct cgccgctccg ctgctgacgc cggacggaac ttgcctcttc ctcaagggca
     8581 ccaatgcaag gacagaattg acgcacgcgg cgacgcaatg gcacatgcag gtgcaaagtg
     8641 tccccagccg gacaatccct gacgcctgca ttcttcggat cagccatctc agccgtgtca
     8701 tcccagaacc ctgacgctac tcgtgtcctc gccgtcgcca accagaaggg cggcgtcggc
     8761 aagaccacga cggcgatcaa cctcgcaacc gcacttgccg caaacaacga gcaggtgctg
     8821 ctgatcgacc tcgacccaca gggcaacgcc tccactggcc tcggcgtccc acgaactgaa
     8881 cgtgccaccg gcagttaccg gttgctgatg ggcgatgcga ccgccaagga tgc
//
LOCUS       DATGEM010000570         6490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1291118, whole genome shotgun sequence.
ACCESSION   DATGEM010000570 DATGEM010000000
VERSION     DATGEM010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291118"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VKQ27_17435"
     CDS             <1..610
                     /locus_tag="VKQ27_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90769.1"
                     /translation="DLLDIVTAEVADPGAAVLIDPEPDDLDAGLAELVAPLPHKLSDT
                     DNKKGSRQLAQLSEEVERIARTLAELSVDESPAEMGSTALSVTSVDGQLVRALLRLRR
                     LRAQHFLPALFADPAWDILLDLAAARIERQRVAVSSLCIAVAVPATTELRWITQMTDQ
                     HLLVRQPDPRDGRRVFIGLSEQAALAMDNYLATARRIVAPVG"
     gene            658..732
                     /locus_tag="VKQ27_17440"
     tRNA            658..732
                     /locus_tag="VKQ27_17440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:690..692,aa:Val,seq:tac)
     gene            complement(1436..2323)
                     /locus_tag="VKQ27_17445"
     CDS             complement(1436..2323)
                     /locus_tag="VKQ27_17445"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLY90770.1"
                     /translation="MILDHLGFSPGVVDGKAGSTLKLALSGFQTAKGLPVTGDLDPAT
                     MRIFAQYHAVGAIRTVTLTPADLAGPFVGPIPKRENDQAKLPSLGYANPLEMLAERYH
                     TTPATLMALNSPAMKLIAGNVIHVPDTTTWTRDYPASLPDGYKQTLAGLNVGSSQPRA
                     AKLVVSNAAKSVSVYDAAGKLLAQFPVTTGSRHDPLPIGEWKIVGTSYNPEFHFNPKL
                     FWDAKKDDKATTLQPGPKGPVGIVWLGINKPHYGIHGTPQPQNIGRTESHGCIRLTNW
                     DAGRLSLMVKPGTPAVFQP"
     gene            complement(3147..3692)
                     /locus_tag="VKQ27_17450"
     CDS             complement(3147..3692)
                     /locus_tag="VKQ27_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLY90771.1"
                     /translation="MDYLDALLWRNGEFADQGFIPDLKMMPSGKLLIIGCVDPRVDPE
                     TIFKLESGEAAVIRNVGGRVNPAALETIKLLRAVAQAAGGSTGPAPNLIVLHHTDCGI
                     THCQHYTPGLLAQHMGVAPDELDAQAIADPYEAVALDVAALRADPDVAARYTVSGLVY
                     DVATGKVEMVVPPTRLPPEEA"
     gene            complement(3852..4379)
                     /locus_tag="VKQ27_17455"
     CDS             complement(3852..4379)
                     /locus_tag="VKQ27_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90772.1"
                     /translation="MPMIDATIPEGAFTPDVEARLLNEITDILIRAEGFDPSNPVARR
                     LSFLFLHRPAAIYVGGLPATAPRYRITTSVPEGKYTDEAIKTLVREITEAFVRAEGGS
                     FEDIAPRVWIFPTEIPEGRWGGNGVINGIGGIQALINGEAERAIGEEQLATRRRAKAL
                     EMIGGLADAVQRGAR"
     gene            complement(4404..5903)
                     /locus_tag="VKQ27_17460"
     CDS             complement(4404..5903)
                     /locus_tag="VKQ27_17460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90773.1"
                     /translation="MLLSVSVPSFMINLDSNIVAVSLPSIARSLHADFAAIEWVISTY
                     TLMFASLVMPAGALADRYGRKRVLVSGLAIFTLASLICGAAPTVLILNVARAFQGLGA
                     ALQLSAALAILSHGFRGTERARAFAFWGSVIGIAMTLGPVTGGIITQRFGWQWAFYVN
                     IPVGIVIIGIILRSVRESRDPEAVGVDVPGLLSFSASLFLLTFGLISGNHRGWTSATV
                     LAELIGAAALFATFLFVETRRSRPMLDLSFFKRSTYIGSNVTGVAFAACFLTMLTYLP
                     LYFQGGLSASPTRAGLLMLPMAIPLFAGPRVVSAYLTYKFSGRTLLAVGLALVSTGLF
                     WMAMVAPLFRYEAMLVGMMIAGCGAGILNGESAKVGMTVIPPERAGMASGVGGTVRFS
                     GVVIGFAALGAVLYQRIAGVIGEAFGPGHGAAVATLTRAIASGKLTPGSGAALASHAF
                     GAGYQTLLFSAATFAGLSTLISWFCVRADETQPLARRDSAHATLVLPVE"
     gene            complement(5957..6358)
                     /locus_tag="VKQ27_17465"
     CDS             complement(5957..6358)
                     /locus_tag="VKQ27_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY90774.1"
                     /translation="MPSSGVIAAIGLALASSSFALPAAQAQSVGHRTDLQRHDLSIPG
                     YEAVQVRVDLDPGATAPIHRHPGEEIIYVLEGTMEYRLQGRPPMTLKTGDVLFVPAGV
                     AHSATNVGTGNASELATYIVEKGKPLVELVK"
BASE COUNT         1169 a         2038 c         2165 g         1118 t
ORIGIN      
        1 cgatctcctc gacatcgtga cggcggaggt cgccgatccc ggtgcggcgg tactgatcga
       61 tcccgagccg gacgatctcg acgcggggct ggcggaactg gtcgccccgc tgccgcacaa
      121 gctgtccgat accgacaata agaagggaag ccggcaactc gcgcaactca gcgaggaggt
      181 cgagcggatt gcccggacgc tggcggagct gtcggtggac gagagcccgg cggagatggg
      241 gtccacggcc ttgtcggtca cgtcggtgga tgggcagctg gtccgcgcgc tcctgcgtct
      301 ccgccggctg cgcgcgcagc atttcctgcc ggcgctgttc gccgatcccg cctgggacat
      361 tctgctggac cttgccgcgg cgcggatcga gcggcagcgg gtggcggtgt cgagcctgtg
      421 cattgccgtc gcggtgcccg ccacgaccga attgcgctgg atcacgcaga tgaccgacca
      481 gcatctgctg gtccgtcagc cggatccacg ggacggccga cgggtattca tcgggctgag
      541 cgagcaggcg gcgctggcga tggacaatta tctggcgacg gcgcggcgga ttgtcgcgcc
      601 ggtgggctga catggcttgc cgatcgcgcg cggagcgggc ataggcccgc gccatgtggg
      661 cggttagctc agcggtagag cggctcgttt acaccgagcg ggtcgggagt tcgatcctct
      721 caccgcccac cattccctgc cgaagtgttg agcacagata agagtgcctc attcgtgaga
      781 tatgggtgcc tcagcgcaat ggctgacgtg gggacattcc ggtcaactgg acgacgatcg
      841 cggccgagct gcagcggaac tggaccctgc cgaccgggca gaagatagag aaactcttcc
      901 acaaccatta cccgccctac gggctgctcg tggatagctt cgaggctgcg gctgcggtca
      961 ggtaggcgtc ctagccgctc gcgcccttct ccgggcgatc cgacgggggc tcgatctcaa
     1021 tctgtcgatc gggccctcgc atagagccgc accttcctcg gaggttcacg ttgatcctcg
     1081 gtccatggcc tccacgtcca gtccgtgcgt ggacaccgtg agaacgcggt ttgattgaca
     1141 agtcattgcg tggtgagacg acacgtgatc gcagtacgtc gtctctgcta tttcttggcc
     1201 gcagccgagc atggcagctt cgcaaagctg ctttgacgct cgagatgcgg ggagtcggcg
     1261 aagagccgct cgacagcaaa gcacccaaat ctcgtcttta ccgggctgat tgccgactcc
     1321 cgataccggc cattcgttca cctggcgcgg ataagggcag cgagcgcccc cttcatgcca
     1381 ttttgcgact gctcgtgtgc cagcaacggc acggtgatga caattccgaa gcgggtcatg
     1441 gctggaatac ggccggtgtt cccggcttca ccatcagcga gagacggccg gcgtcccagt
     1501 tggtcaggcg gatgcagcca tggctctcgg tgcgcccaat gttttgcggc tggggtgtgc
     1561 cgtgaatccc gtagtggggc ttgttgatgc cgagccagac gatgcccacc ggccctttcg
     1621 gccccggctg cagggtcgtc gccttatcgt ccttcttcgc atcccagaac agcttggggt
     1681 taaagtggaa ttccgggttg taggaggtgc cgacgatctt ccattcgccg atcggcaggg
     1741 ggtcgtgtcg gctaccggtc gtcactggaa actgcgcgag cagtttgccg gcagcgtcat
     1801 agacggacac cgacttagcc gcattcgaga caaccaactt cgccgcccgc ggctggctcg
     1861 atccgacatt gagccccgca agcgtctgct tgtagccgtc cggcaggctc gccggatagt
     1921 cgcgggtcca ggtggtggtg tccggcacat gaatgacgtt gccggcaatg agcttcatag
     1981 cgggcgagtt gagcgccatc agcgtggcgg gcgttgtatg atagcgctcc gccagcattt
     2041 ccagcggatt ggcatagccg agcgaaggga gcttggcctg gtcattctcg cgcttcggaa
     2101 tcggacccac gaacggcccg gcgagatccg ccggcgtgag cgtcacggtg cggatggcac
     2161 cgacagcatg atattgtgcg aatatccgca tcgtggcggg atccagatca cccgtgaccg
     2221 gcaacccctt cgctgtctgg aacccggaga gggctagttt cagcgtgctg cccgccttgc
     2281 cgtcaacgac cccgggtgag aaaccgagat gatcgaggat cacctggacc tgtatgagct
     2341 tgaggtctgg cttccggccg gagggcggaa gggctggctt agtcttggcc gctgccgccg
     2401 gtggcgctgt gggttttgcc ggagctgccg ggctgaccgt ccccaaaaga ccaaccgata
     2461 tcaccagaag cccgttacgc cggctgaaca tgcattccct tttcatcatg acggcagaca
     2521 agcccttccc gcacgccgga cgatccttgg gtgtgctgcc ggccggcccg ggctccagac
     2581 ggaccacgcc tttctgcgga cagccgcctc ctagcgactt ccaggatgac gatcacgtag
     2641 agtttaacgc accggtgagt gcaagcggaa tgggacgttt gccgacctag caccgcgccg
     2701 ccgaaggtcg gctttcccca cctccactcc tgagcccgga caggcggaaa acggcgagaa
     2761 ttcccctaaa cgcggtcagg cggcagtgcg cccaaagaac gtccaagact gtgaaggata
     2821 tcgtacgcct gtcggcaccc gatatccttc acagtcttgg acgttcgcgc agcgggtgtt
     2881 gctgctcgaa agcggacgct gcgcttcagt cacgggcctg ccgaagttga ggggaggcac
     2941 tcagttcgta tcaggacatt gacttggccg agacgggctg ggcgatcctc ctccggagcg
     3001 tgcgcgatct ggccgtcagt agttgtcagg agactgcctc accgatcgtg gcgtctggcc
     3061 cagcccccag cgccaggcgc gcgatagaag aggcctcgtt ccggtcgacc ggcgcccgtg
     3121 atgacgccgg ctgacaggct gggggatcag gcttcttcgg gcggcagccg cgtcggcggg
     3181 acgaccattt cgaccttccc ggtggcgacg tcgtagacga gccccgacac cgtgtagcgg
     3241 gcagcgacgt ccgggtcggc cctgagcgcg gccacgtcga gcgctaccgc ctcgtagggg
     3301 tcggcgatgg cctgcgcgtc aagctcgtcg ggcgccacgc ccatatgctg cgcgagaagc
     3361 cccggcgtgt agtgctggca gtgcgtgatg ccacaatccg tgtggtgaag gacgataagg
     3421 ttcggcgccg ggccggtgct cccgccggcg gcctgcgcca ccgcgcgcag gagcttgatc
     3481 gtctcgagcg ccgccgggtt cacgcggcca ccgacattgc gaatgacggc ggcctcgcct
     3541 gactccagct tgaagatggt ctccgggtcg acgcggggat cgacgcaacc gatgatgagt
     3601 aacttgcccg acggcatcat cttcaggtcg gggatgaagc cttggtcggc gaactcgcca
     3661 ttgcgccaca acagggcgtc cagataatcc atcatattct ccatcaattg ggggatgagc
     3721 gagtttcagt tccgctgggg agacgggtcg acatcatcgc ctgggcggcc ttgggatcgg
     3781 ccgtgaacgc ctggtggatg ctgcgagact gggcgcacgc gatggtctcg tcgttagggt
     3841 cagcctcaat ctcaacgcgc gccgcgctga acagcatcgg caagaccacc gatcatctca
     3901 agcgccttgg cacgacgacg agtggcaagc tgctcctcgc cgatcgcccg ctcggcctcg
     3961 ccgttgatga gcgcctgaat gccgccgatc ccgttgatga cgccattgcc gccccaacgg
     4021 ccctcgggga tttcagtcgg gaagatccat acccgcggcg cgatatcctc gaagctgccg
     4081 ccctcggcgc ggacgaaggc ttcggtgatc tccctgacca gcgtcttgat ggcttcgtcg
     4141 gtatatttcc cctccggaac gctcgtcgtg atccggtagc gcggcgcggt tgcggggagg
     4201 ccgcccacat aaatcgcggc cggccggtgc aggaaaagaa agctgaggcg ccgggcgacc
     4261 gggttgctgg gatcaaagcc ttcggcccgg atcagaatat cggtgatttc attcagcagc
     4321 cgggcttcga cgtccggcgt gaaggcgcct tcagggatgg tggcgtcaat catgggcatg
     4381 gggagacttc cttgcttggg ggttcattcg acgggcagaa cgagggttgc atgggcgctg
     4441 tcgcgcctcg cgagcggctg ggtttcatcc gcgcgcacgc agaaccagga gatgagggtc
     4501 gacaggcctg cgaaggtcgc cgccgagaac agcagcgtct gatatccggc tccaaaggcg
     4561 tgggaggcca gcgccgcacc gctgccgggc gtcagtttcc cggaggcaat cgcgcgcgtc
     4621 agcgttgcga ctgcggcgcc atgtccgggg ccaaacgcct cgccgatcac gcccgcgatc
     4681 cgctggtaga gcaccgcccc cagcgccgcg aagccgatca cgacaccgct gaagcgaacc
     4741 gtcccgccga cgccggaggc catgccggcg cgttccggcg ggatcacggt catgccgacc
     4801 ttggcggact cgccgttcag aatgcccgcg ccgcatccgg cgatcatcat gccgaccagc
     4861 atcgcttcgt agcggaaaag cggcgcgacc attgccatcc agaacaggcc ggtcgacacc
     4921 agcgcaagac cgaccgccag cagcgtccgg ccggaaaatt tgtacgtcag ataggcagag
     4981 acgacgcgcg gccccgcaaa cagcgggatc gccatgggca gcatcagcaa gccggccctg
     5041 gtcgggctcg cgctcaaccc gccctgaaaa tacaaaggga gataggtcag catggtcagg
     5101 aaacaggccg cgaacgcgac gccggtgacg ttcgatccga tataggtgga gcgcttgaag
     5161 aagctcagat ccagcatcgg gcgtgagcgg cgtgtctcga cgaagaggaa tgtggcgaac
     5221 agcgcggccg ctccgatcaa ctccgcgagc acggtggccg acgtccaccc gcgatggttt
     5281 cccgagatca ggccaaacgt aagcagaaac aggctggcgc tgaagctcaa cagcccggga
     5341 acatcaaccc cgaccgcttc cggatctcgc gactcccgta cggatcttag gatgatcccg
     5401 atgatcacga tgcccactgg tatgttgacg tagaacgccc attgccatcc gaacctctgc
     5461 gtgatgatcc cgcccgtgac cgggccgagc gtcatggcga tgccgatcac ggaaccccag
     5521 aaggcgaagg cgcgggcacg ctcggtgccc cggaatccat gagacaggat cgccagcgcg
     5581 gcgctcagtt gcagggctgc tccgagcccc tgaaacgcgc gcgccacatt gaggatcaac
     5641 acggtgggcg cggctccgca gattagcgaa gcgagcgtga aaatggcgag cccggagacg
     5701 agcacccgct tgcggccata gcgatccgcc agcgctcccg caggcatcac cagcgaggcg
     5761 aacataagcg tgtaggtgct gattacccac tcgatcgcgg cgaagtcggc gtgcagcgag
     5821 cgggcgatcg acggcagcga taccgccacg atgttcgagt cgagattgat catgaaggac
     5881 ggcaccgaca cgctcagcag cacgagcaac ttctctgtgg cgccgaggat ctcggccgcc
     5941 tcgccccgcg gatggctcat ttcacgagct cgaccagcgg tttgcccttc tcgacaatat
     6001 aggtcgccag ctccgatgcg ttgccggtgc cgacattggt ggcgctgtgc gcaaccccgg
     6061 cgggcacgaa caggacatcg ccggtcttga gcgtcatggg cggccggccc tggagccggt
     6121 attccatcgt gccttcgagg acatagatga tctcctcgcc cggatgccga tgaatggggg
     6181 cggtggctcc cggatcgagg tcgacgcgaa cctggacggc ctcataaccg ggaatgctga
     6241 gatcgtggcg ctggagatcg gtgcgatggc ccaccgactg ggcctgcgcg gcagggagcg
     6301 cgaagctgct gctcgcgagg gccagaccga tggcggcgat gacacctgaa ctaggcatgg
     6361 atctgtcctc gcggagagcg ggaaaacggg cgtaacgggc cgctcagcgg ttgttgtgcc
     6421 agaaggtcag catcgcgtcg gtgaactggg cggggcattc ttccggcagg aagtgaccgc
     6481 agcccggaag
//
LOCUS       DATGEM010000571         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_740484, whole genome shotgun sequence.
ACCESSION   DATGEM010000571 DATGEM010000000
VERSION     DATGEM010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740484"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VKQ27_17470"
     CDS             <1..932
                     /locus_tag="VKQ27_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HLY90775.1"
                     /translation="YATYIKDRLDGFGGESRPLTYQDLVEFPELARRVFGDPGRSRRK
                     TPGCNGPISVRDPAAAETDTKNLKAALAEAKVEEAFLSAASPGLISLFFRDSHYGSHE
                     AYLAAIAEAMRQEYETVVAAGFILQLDCPDLAMGRHIQNAHLTTAEFRKAAGLHIEAM
                     NHALRNIPADRARVHLCWGNYEGPHHCDVPLADIIDVIFTAKPAGLSFEAANPRHAHE
                     WKLFETVKLPDGKVLIPGVIESKTNFIEHPELIAQRIGRYAALVGRENVIAGSDCGYG
                     TWVGQAAVDPQVVWAKLKSLAEGARLASGEFWA"
     gene            complement(942..1352)
                     /locus_tag="VKQ27_17475"
     CDS             complement(942..1352)
                     /locus_tag="VKQ27_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90776.1"
                     /translation="MLDADPKTDWSKIDLEALRQHLIDMNEVTLKAAVSPKTIDGGLE
                     MSITGDGRTLAAIQRMVPAWAETMNGHKGWNTKAAALPDGELLTVTATDPREVQHIRG
                     LGFIGLLVSGSHHQMHHLMMAKGEFDPEHAAHMH"
     gene            complement(1647..2363)
                     /locus_tag="VKQ27_17480"
     CDS             complement(1647..2363)
                     /locus_tag="VKQ27_17480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90777.1"
                     /translation="MTSGSEAAPLPNLGPDVYAKWRASGIGAITEQLQRRLILALLGD
                     VTGRHVLDVGCGDGDLAVELRRRGAIVTGIDASSDMIETAKARANREGLDIDFQIGAA
                     EHIPFAAGRFDTVVAVTILCFVENVAPVFHEIARVLRPGGVLVIGELGKWSLWAAARR
                     IRAWLGSPLWRRGRFRTSGELRRLVERAGLTPGPVHGAIYYPRWQRAAQLLAPCDAAF
                     SRLTTLGAAFLALSAIKHPR"
     gene            complement(2434..>2673)
                     /locus_tag="VKQ27_17485"
     CDS             complement(2434..>2673)
                     /locus_tag="VKQ27_17485"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY90778.1"
                     /translation="RGRMELRNIGSARERVLQYLRLRAGALSRSVALEGQLQDIAAEI
                     GVTRETLYRTLAALEDEGQLARSGSAILLKKTHNL"
BASE COUNT          483 a          888 c          826 g          476 t
ORIGIN      
        1 gctacgccac ctacatcaag gaccggctcg acgggttcgg tggcgagagc cgtccgctga
       61 cctatcagga tctcgtcgag ttccccgaac tagcgcggcg tgtgttcggc gatcccggcc
      121 gctcgcggcg caagacgccc ggctgcaatg ggccgatctc ggtgcgcgat cctgctgccg
      181 ccgagaccga cacgaagaac ctcaaggccg cgctggcgga ggccaaagtg gaagaggcat
      241 tcttgagcgc cgcctcgccc ggcctcatct cgctgttctt ccgcgacagc cactacggca
      301 gccacgaggc ctatctcgcc gccatcgccg aggcgatgcg ccaggaatac gagacggttg
      361 tcgccgctgg cttcattctc cagctcgatt gccccgatct ggcgatgggg cggcacatcc
      421 agaacgcgca tctcaccacc gccgagttcc gcaaggcggc ggggttgcac atcgaggcga
      481 tgaaccacgc gctgcgcaac atcccggccg accgcgcccg ggtgcatcta tgctggggca
      541 attacgaggg gccgcaccat tgtgatgtgc cgctggccga tatcatcgac gtcatcttca
      601 ctgcgaagcc cgccgggctc tccttcgagg cggccaaccc acgccacgcg catgagtgga
      661 agctgttcga gacggtcaag ctgccggacg gcaaggtgct gatccccggg gtcatcgagt
      721 cgaagaccaa ttttatcgag cacccggagc tgattgccca gcgcatcggg cgctacgccg
      781 cgctggtcgg acgcgagaat gtcatcgccg gcagcgattg cggctacggc acctgggttg
      841 gtcaggccgc ggtcgatcca caggtggtgt gggccaagct caagagcctc gcagagggcg
      901 cgcgtctggc ctcaggcgaa ttttgggcat agcggcctcg gtcagtgcat atgcgctgcg
      961 tgctcggggt cgaactcgcc cttggccatc atcaaatgat gcatctggtg atgtgagccg
     1021 ctcaccagca ggccaataaa gccgagcccg cggatatgct ggacttccct gggatcggtt
     1081 gcggtgacgg tgaggagttc gccgtccggc agagccgccg ccttcgtgtt ccaacccttg
     1141 tggccgttca tcgtctcagc ccaggcggga accatccgct ggatcgccgc gagcgttctg
     1201 ccatcgccgg tgatgctcat ttcgagacca ccgtcgatcg ttttcggaga taccgctgcc
     1261 ttcagcgtca cctcgttcat gtcgatgaga tgctgacgca gcgcttcgag atcgattttc
     1321 gaccaatcgg tcttcgggtc ggcgtccagc atctcgacaa tttcctggat cgcgccgaag
     1381 gcgtcctgac caggcagctt gggcaccgtt gcgcctatgc tcatggccgg gtgattgtcc
     1441 atcgggtgcc cgctctgggc gagagccgca ccagcgccga cggacgctag cgccgcaacg
     1501 gccgcaccaa tggcgatcag gtgacggacg caatgcatgg cgagcctctg tcaaatcgaa
     1561 ctgtcgatca gcctacctgg cgagccttcg atccccacat gatcaggatc atgcgtctct
     1621 ataaaatctc ggcgcggctg gtccggtcat ctcgggtgct tgattgctga gagtgccagg
     1681 aacgcagccc ccagcgtcgt cagtcggctg aaggcggcgt cacaaggcgc caagagctga
     1741 gcggctcgtt gccatctggg ataatagatc gccccatgaa ccggccctgg cgtcaaaccg
     1801 gccctctcca ccagtcgtcg aagttcgccg gaggtcctga accgcccgcg ccgccacagg
     1861 ggcgatccga gccaggcgcg aatccgacgc gcagccgccc acaggctcca cttccccaac
     1921 tcgccgatga ccagaacccc tcccggtcgc agcactcgcg cgatctcgtg aaagacgggg
     1981 gctacattct caacgaagca gagaatggtc acggcaacga cggtatcgaa ccgtccagcg
     2041 gcgaacggga tgtgttcagc cgctccgatt tggaaatcaa tgtccaggcc ttcccggttg
     2101 gccctcgcct tggccgtctc gatcatgtcg gacgacgcat cgataccagt gacgatggcg
     2161 ccgcgccgcc gtagttccac cgccaggtcg ccgtcaccgc atcccacatc gagcacgtgg
     2221 cgtcccgtaa cgtcgccgag aagagcaagg atcagccgac gctggagctg ctcggtgatg
     2281 gcgccgatgc cagaggcccg ccatttcgcg tagacatccg gtcccaggtt cggtagcgga
     2341 gcagcttcgg accccgaagt catcccagag tacccaccca tgctggcgtg ctgggacgct
     2401 aacgcagccg agccgtggca cgcatgatct ggatcatagg ttgtgggttt tcttgagcag
     2461 gatggcagat cccgaacggg ccaactgccc ttcgtcttcg agcgcggcaa gggttcggta
     2521 aagcgtctcg cgagtcacgc cgatctccgc ggcgatgtcc tgcaactgcc cctcaagcgc
     2581 gacacttcgg ctcagagcgc cggcgcgcag ccggagatac tgcagaacgc gctcccgcgc
     2641 cgagccgata ttgcgcaatt ccatccgtcc tcg
//
LOCUS       DATGEM010000572         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_692539, whole genome shotgun sequence.
ACCESSION   DATGEM010000572 DATGEM010000000
VERSION     DATGEM010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692539"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..918)
                     /locus_tag="VKQ27_17490"
     CDS             complement(10..918)
                     /locus_tag="VKQ27_17490"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90779.1"
                     /translation="MNVAATRDLALHRLMQAVPIETCSALGAELGLWLGQRSDRASDT
                     RAKAILRMLHPEADRLAIDRQARQLWCNIGRTFAEFSALPRIAAERRVTISSEAMIAR
                     IAPDPRPVIVCFAHLGNWEALGAALCGHPTVIAHRRMTAVIAPPANQARAAIAARMRG
                     MLSVEQLHIGPGVWRAVLTRLRQPFGTVWLAVDAAEGGVDTPLFGRPPRPDGTLGKIA
                     RLAASTGALILPVYCERLPNVQFIAHVLEPCEPPATPVDTADLLQHVVHLNALFDTPV
                     RRLIDQWYMAIEFPSDAASAATIERH"
     gene            complement(915..2189)
                     /locus_tag="VKQ27_17495"
     CDS             complement(915..2189)
                     /locus_tag="VKQ27_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLY90780.1"
                     /translation="MARRNLLEIWPEATFRQTHFEHALFRRRIFVCNSPETVRQVFVE
                     DAANVMRKSSQQVHALKSLIGDGLFISDGELWQHRRKTVAPLTHISRLGEFVPAMAAA
                     AAERAAMWQAKEGAEFDVLAEMAELTADVICRAVFGTRLGQAHAAEVVRGFSSFQSAV
                     EQTDLVSLLGLPEFLPRLSGRHVRQSTRRIHNVVDSLLHDMLTDDGLAEPSLAHALAA
                     ALGDAGALRNETSVLLMAGHETTANTLAWAWYLLSQDDATWQRLTQEADVLADAPASL
                     EDLQHLTFTRAVIDETLRLYPPVPLQARRSAEARRIADRDVPANSIILLVPWLLHRHR
                     LYWDQPDTFLPERFLPDGNGVPNRYAYVPFSIGPRVCTGAAFALTEMVLCLATLARQF
                     RPVLRQGHEVMPVCRLSLRPGTSLPMQMMPRT"
     gene            complement(2258..>2713)
                     /locus_tag="VKQ27_17500"
     CDS             complement(2258..>2713)
                     /locus_tag="VKQ27_17500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY90781.1"
                     /translation="ARHGTAAPQLVIAGAPANRAAPILARIAAALAPRGPVVWARGLS
                     TPALRQVIGHARALLMPSLAEGYGMPVAEALALGTPVVASDIPPHVEIARDHASFCPP
                     NDAAAWCAAIERLAFDEAAHQTARARAAAYAPLPAARYFQRIESFLQQF"
BASE COUNT          480 a          894 c          905 g          434 t
ORIGIN      
        1 gatccacgat caatgccttt ctatcgtcgc ggcggatgcc gcatcggatg gaaactcgat
       61 agccatgtac cattggtcga tgagcctgcg caccggcgta tcgaacagcg cgttcagatg
      121 caccacgtgc tgtagcagat ccgccgtatc cacaggtgta gccggtggct cacagggctc
      181 cagaacgtgg gcaatgaact gtacgttcgg cagtcgttcg caatagaccg gaaggatcag
      241 cgcgccggtt gacgcggcca gccgggcaat cttgcccaag gttccgtccg gccgcggcgg
      301 ccggccgaac agcggtgtat cgacgccgcc ttccgccgca tccacagcca accagacagt
      361 gccaaagggt tggcgcagcc gcgtcaatac agcgcgccat acacctggcc cgatatgcaa
      421 ctgctcaacc gacagcatgc cgcgcattcg ggcggcaatc gccgcgcgcg cctggttcgc
      481 gggcggcgct atcacggcgg tcatacggcg atgcgcgata accgttggat gtccgcagag
      541 tgctgctccc aacgcctccc aattgccgag atgcgcgaag cagacgatga ccgggcgtgg
      601 gtcgggcgcg atccgcgcta tcatcgcctc ggacgatatc gtcacccgtc gctcagcggc
      661 gatgcgcggc agcgcggaga attcggcgaa ggtgcggccg atattgcacc agagctgccg
      721 cgcctgccgg tcgatcgcca acctgtcggc ctcggggtgc agcatccgca atatcgcctt
      781 tgcccttgtg tcggaggctc ggtcagagcg ctggcccaac cagaggccca gctccgcgcc
      841 cagtgccgag catgtctcga tgggcaccgc ctgcatcagc cgatgcagcg ccaggtcgcg
      901 cgtcgccgcc acgttcatgt gcgcggcatc atctgcatcg gcaggctggt gcccggccgc
      961 agcgacagcc ggcataccgg catcacttca tggccttgac gcaggaccgg ccggaactgc
     1021 cgcgccagcg tcgccaggca cagcaccatc tctgtcagcg cgaaagcggc accggtgcag
     1081 acgcggggac cgatgctgaa gggaacataa gcgtagcgat tgggaacgcc gttgccatct
     1141 ggcaggaagc gctccggcag gaacgtgtcg ggctgatccc agtacagccg gtgccgatgc
     1201 agcagccatg gcaccagcag gatgatcgaa ttggcaggca catcgcggtc agcgatccgc
     1261 cgtgcttctg ccgaacgtcg cgcttgtagg ggcactggtg gatacagccg caatgtctcg
     1321 tcgatcactg cgcgcgtgaa tgtcaggtgc tgcaggtcct caagcgaggc cggtgcatcc
     1381 gccagaacgt cagcctcctg ggtcagacgc tgccaggtcg cgtcgtcctg cgacaaaaga
     1441 taccaggccc atgccagtgt attggccgtg gtctcgtgac ccgccatcag cagcacggac
     1501 gtttcgttcc gcagcgcacc agcatcaccc agcgctgcgg cgagtgcgtg cgccagcgac
     1561 ggttcggcca gaccgtcgtc ggtcagcata tcgtgcagca gactgtccac tacattgtgg
     1621 atacgacgtg tactctgccg cacatgccgc ccgctcagcc ggggcaggaa ctccggcagg
     1681 cccaggagcg agaccagatc ggtctgctcc accgctgact gaaaagagct gaagccgcgc
     1741 accacctcgg cagcgtgcgc ctggccaagc cgcgtcccga acaccgcgcg gcagatcacg
     1801 tcggcggtca gctcggccat ctcggcgagc acatcgaatt ccgcgccttc tttcgcttgc
     1861 cacatcgccg cacgctcggc cgcggcagcc gccatcgccg ggacgaattc gcccagccgc
     1921 gatatatggg tgagcggggc gacggtcttg cggcggtgct gccagagttc gccgtcgctg
     1981 atgaacagcc cgtcgccgat cagcgacttc agcgcgtgca cctgctggga tgactttcgc
     2041 atcacattgg ccgcgtcttc cacgaagacc tggcgcacgg tctcggggct gttgcagacg
     2101 aagatgcggc ggcggaacag ggcatgctcg aaatgcgtct ggcggaacgt ggcttccggc
     2161 cagatctcca gcaggttgcg ccgcgccatc ttcagcatct tcaggggccc aggggcgtgc
     2221 ggcgggcgcg gcggaaaggg cggtatgaat gccatggtca gaattgctgg agaaatgact
     2281 cgatgcgttg gaagtatcgt gcggcgggca agggtgcgta ggcggcggca cgcgcgcggg
     2341 ctgtctgatg tgccgcctca tcgaatgcca gccgctcgat tgcggcgcac cacgccgcgg
     2401 catcattggg tgggcagaat gaggcgtggt cccgcgcgat ctccacatgc ggcgggatgt
     2461 ccgaggcgac caccggcgtg cccagcgcca atgcctcggc gacgggcata ccgtatcctt
     2521 cggcgagcga cggcatcagc agggcgcggg catgtccgat gacctggcgc agcgccggcg
     2581 tggacagacc gcgcgcccag accacaggac cgcgtggtgc cagcgccgcc gcaatccgcg
     2641 ccaggatcgg cgcggcgcgg ttggcgggtg cgccggcaat cacgagttgt ggtgccgcag
     2701 tgccgtggcg cgc
//
LOCUS       DATGEM010000573         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_379382, whole genome shotgun sequence.
ACCESSION   DATGEM010000573 DATGEM010000000
VERSION     DATGEM010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379382"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VKQ27_17505"
     CDS             complement(<1..397)
                     /locus_tag="VKQ27_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HLY90782.1"
                     /translation="MAAPMHHILNRFRITTGGDFRLKDFHPSDTCGHLLSREQAEKMV
                     AEDVERLSKLHDRLYALNEWALLCVFQAMDAGGKDSTIKHVMTGVNPQGVQVTSFKVP
                     GPEELAHDFLWRINRALPERGSIGIFNRSH"
     gene            617..1537
                     /locus_tag="VKQ27_17510"
     CDS             617..1537
                     /locus_tag="VKQ27_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HLY90783.1"
                     /translation="MLVATPSGEGFRVLDSFSRIVRLGEGLHHTGELSAAAMERALAA
                     LQACAARLERRPVRRLRAIATEACRRAANGRAFLARVKSETGLDIGVISSREEAELAL
                     ESCAPLLAGWGRRALLFDIGGGSTELAWVRVGATGLPELIGYESLPVGVVTLSERFGP
                     VGFDPDGFEAMVDDIVARLASFEAVHRIAREIRDGGVQMLGTSGTVTTLAGVALGLPR
                     YRRLLVDGTVLTGDAAEQALTNLRALGRDGLEAHPCVGPDRVDFVLPGCAVFAALVRM
                     WPAPRVVVADRGLREGMLLRMIRADRRRRP"
     gene            1554..2207
                     /locus_tag="VKQ27_17515"
     CDS             1554..2207
                     /locus_tag="VKQ27_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HLY90784.1"
                     /translation="MKSPRGLTERVRTAKGRSTASQRWLARQLNDPYVAAAKQQGWRS
                     RAAFKLIELDERFKLVRRGAKVVDLGAAPGGWSQVAVQRGARVVAVDLLAVDPIAGAV
                     VLQGDFTDEAVQARLLAELGGKADLVLSDMAPNTTGHTATDHLRIVALAEEALEFALD
                     VLGEGGAFVAKVFQGGAERAMLERLKRHFGAVRHAKPPASRKESSELYVVATGFRRA"
     gene            complement(2247..2684)
                     /locus_tag="VKQ27_17520"
     CDS             complement(2247..2684)
                     /locus_tag="VKQ27_17520"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLY90785.1"
                     /translation="MATIPKTLQPHINTAFPDFVCLVGTVQPDGYAQVSPRGSTMVFD
                     DDHLALWERGKGSTTAAIRDGTKVTVYFRKPQLRADGVLPKGGIARFYGTAKVVKSGP
                     QYEQVWTKLVQPEKDRDPEKKGFAVLITIERAEDLDGMALKLD"
BASE COUNT          416 a          799 c         1069 g          506 t
ORIGIN      
        1 agtggctgcg gttgaagatg ccgatggagc cgcgttccgg gagtgcgcgg ttgatacgcc
       61 acaagaagtc atgcgccagc tcctcgggcc cggggacttt gaacgatgtg acctgcacgc
      121 cctgcgggtt cacgccggtc atcacgtgct tgatcgtgct gtccttccca ccggcgtcca
      181 tcgcctggaa cacacagaga agagcccact cgttcagggc gtagaggcga tcgtgcagct
      241 tggacagccg ctcgacgtct tcggcgacca tcttctccgc ctgctcgcgc gagagcaggt
      301 ggccacacgt gtcggacggg tggaaatcct tgaggcggaa atctccgccg gtcgtgatcc
      361 ggaagcggtt gaggatgtgg tgcattggcg cggccatcgt cggtctccct gacagcgggg
      421 agatgggggc cggatgaaat tcaggcaacc taatgtaacg gcgcttgccg ggttcttcag
      481 gcaagatgac aatacttcga tgctgccccg ggctgaccag tatctggccg aagctggcgc
      541 gcgtcgggcg catgacccga ggcgcgtcca ggcgccgacg tttgccgcgc tggacctggg
      601 cacgaacaac tgccgcatgc tggtcgccac cccatcaggg gaagggtttc gggtactgga
      661 cagtttcagc cggatcgtgc ggctgggtga agggctgcat cataccggtg aactgagtgc
      721 cgctgccatg gagcgcgcgc tggcggctct gcaagcctgc gccgcgcggc tggagcggcg
      781 gcctgtgcgg cggctgcgtg cgatcgcgac cgaggcgtgc cgccgggccg cgaatggccg
      841 cgctttcctg gcgcgggtga agagcgagac ggggctggat atcggcgtga tctcgtcccg
      901 cgaggaagca gagcttgcgc tcgaaagctg cgcgccactg ctggcaggct ggggtcgccg
      961 ggcgctgctg ttcgacatcg gcggcggttc gaccgaactg gcctgggtgc gcgtcggagc
     1021 gacgggcctg ccggagctga tcggctacga atcactgcct gtcggcgtcg tgacgctatc
     1081 ggagcggttc ggaccggtgg gcttcgatcc ggatggcttc gaggcgatgg ttgatgacat
     1141 tgtcgcgcgg ctggccagtt tcgaggcggt gcaccgcatt gcccgggaga tccgggacgg
     1201 cggcgtccag atgctgggca ccagtgggac ggtgacgact ttggcgggcg ttgcgctcgg
     1261 tttgccgcgc tatcggcgac tgctggtcga cggcaccgtg ctgactggcg atgcggccga
     1321 acaggcgctg acaaatctgc gggcgttggg acgggacgga ttggaggctc atccgtgcgt
     1381 cggccctgat cgggtcgact tcgtgctgcc gggctgcgcg gtgttcgcgg cgctcgtccg
     1441 gatgtggccg gcgccacggg tggtggtggc ggatcgcggg ctgcgggagg gcatgctgct
     1501 gcggatgatc cgagccgatc gccgccgccg tccctgagcg ggaggcgaac cagatgaagt
     1561 cgccgcgcgg gctgacagaa cgtgtccgta cggcaaaggg ccgcagcacg gcgtctcagc
     1621 gctggctggc gcggcagttg aacgatccct acgtagcggc ggcgaagcag caggggtggc
     1681 ggtctcgtgc ggcgttcaaa ctgatcgagc tggatgagcg gttcaagctg gtgcggcgtg
     1741 gggcgaaggt ggtggacctg ggggcggcgc ccggagggtg gtcacaggtt gcggtgcagc
     1801 gaggggcgcg cgttgtggcg gtagacctgc tcgcggtgga cccgatagcc ggggcggtcg
     1861 tgctgcaggg ggacttcacc gacgaagccg tccaggcgcg gttgctggca gagcttggcg
     1921 gcaaggctga cctcgtgttg tcggatatgg cgccgaacac cacggggcac acggcgacgg
     1981 accacctgcg gattgtcgcg ctggccgagg aggcgctgga gttcgccctg gacgtgctgg
     2041 gagaaggcgg ggcgtttgtt gccaaggtgt tccagggggg cgcggagcgg gcgatgctgg
     2101 aacggctgaa gcggcatttc ggcgctgtgc ggcacgccaa gccgccggcc agccggaagg
     2161 agtcgtccga actttatgtt gtggcgacgg ggttcaggag agcgtgacag gccccctccc
     2221 gcgcgttgca cgggagggga agtccttcag tcgagcttca gggccatgcc gtccagatct
     2281 tcggcacgct cgatcgtgat caggactgcg aagcctttct tctcgggatc gcggtccttt
     2341 tcgggctgaa ccagcttggt ccagacttgc tcgtattggg ggccggactt cacgaccttg
     2401 gcggtgccgt agaagcgggc gatgccgcct ttcgggagca cgccatctgc gcgaagttgc
     2461 ggcttgcgga aatagaccgt gaccttggtg ccgtctctta tcgctgcggt ggtggagccc
     2521 ttgccgcgtt cccacagtgc gagatggtca tcatcgaaca ccatggtgct gccgcgtggg
     2581 ctgacctgcg cgtagccatc gggctgcacg gtgccgacga ggcagacgaa atcagggaac
     2641 gccgtattga tgtgcggttg cagggtcttc gggatggtgg ccacggtggc ggtctctctg
     2701 ctggttgatc aggctacctt ggggacgaac gcgcggctgg taaagtgctc gtcgccgctg
     2761 gcgaagcgca ggccggcggc tttcaccgcg
//
LOCUS       DATGEM010000574        14169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_721053, whole genome shotgun sequence.
ACCESSION   DATGEM010000574 DATGEM010000000
VERSION     DATGEM010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14169
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721053"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="VKQ27_17525"
     CDS             complement(<1..1836)
                     /locus_tag="VKQ27_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLY90786.1"
                     /translation="MIRRILEPALTHTVTALAATIALAVTLMLPSTYFLSASAARHAE
                     VAAESKVASAVISQLINTNPELWAFENARIRGLLAMLGPLQDTERRAVFFGRDQLVAE
                     QGDSVLPPLMTAGTPLYDSGNIVGRVEIQRSQRQLWLVTAAIAAFAASLGAVTFAVLR
                     SWPLRLLQRALARSTHLATHDTLTGLPNRALFHDRVEQELAWCRRDGSMLAILYLDLD
                     RFKEVNDTLGHAAGDRLLVGVAGRLRTCIRETDTLARLGGDEFAIIQAGIRQLADIEM
                     LAQRLIDAMDQAFDLAGNQVNVGVSVGIALRRATELAVSNADPGILLQEADTALYRSK
                     EEGRGVYRFFATEMNEKLLERRALESDIVTALAQGQFRLHYQPQFDLAERRVVGAEAL
                     LRWHHPVRGEVRPEAFIPLAEDCGLIVRIGEWVLREACRQAARWPSLNCMAVNVSPVQ
                     FRRPGFVDQVKNALARVGIDGRRLELEITEGVLLHETEETLATLRRLHAIGVTIAMDD
                     FGTGYSSLGYLQKFPFDKIKIDRSFVAALQTDSHAAEIVRAVLRMSHAMGIRVNAEGV
                     EQEAQANMLMEEGCEEVQGFLFGRGLGAEDFSALLAQNTPATMPRE"
     gene            complement(1833..2723)
                     /locus_tag="VKQ27_17530"
     CDS             complement(1833..2723)
                     /locus_tag="VKQ27_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLY90787.1"
                     /translation="MRNIGPLSRRAAVAGALQRRALFTRAVAAVLAAAPLFARMTPAA
                     AGTQLRVGGTGMALAPIREIGTAFTATNPDVAVNVLPSLGTGGGLNAVAAGAIELALA
                     ARALNDAERSKGLQAMPYARTPIAFAAHPDAGIREITLAQVAQIFRGQLRNWPNGKAI
                     RLIRREPSDADWSMLRAASPDMTDAIKIALDRPGLLTVSTDQENADVLERLPGAFGAM
                     SLGQIRAERRNVVPLTLDGEPPTTEDLAANRYKLSRTLYVVWRDQPRVEAAAFVTFLA
                     TPQAKDILARLGHIPLAGIA"
     gene            2937..3869
                     /locus_tag="VKQ27_17535"
     CDS             2937..3869
                     /locus_tag="VKQ27_17535"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLY90788.1"
                     /translation="MSATAAMWPAGAAYIDGRFMPIAEAMIPVTEWGYRRSDVTYDVV
                     SVWDGAFFKLADHLKRFRTSMEHYRLQPKESDDDIRDVLHELVRRTGLREAYVAMDCL
                     RGSAPAHLRRHPSNARNYLLAFATPYVWLMPKDVIERGAHLIVASTPRIPEACVNPRA
                     KNFHWADMTKALFEAQDKGADNPVLLDLDGNVTEGPGFNVFAVTDGVVATPDRGVLEG
                     ITRLSVIQMCEKLGVKCEVRTVPLQELRNADEVFISSTAGGVMGVTRLDERTFGNGRP
                     GPVTERLHESYWRFRREGWDCEPVDYVMRAEAAE"
     gene            3880..4737
                     /locus_tag="VKQ27_17540"
     CDS             3880..4737
                     /locus_tag="VKQ27_17540"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLY90789.1"
                     /translation="MDDLAALRLQIEWGADEALEDAPVDRVHRVQVHAPAALAPPPRI
                     QSGAVSSPAPSVEEGVRRALSRGVGELGDTAVPSRAGAAERALQVAAGASTLEELRAA
                     VAAFDGCALRDTASHLVFAAGDPAASLLVIGEPPGADDDRSGAPFAGAAGAYLDRMLG
                     SIGLARDALLLTPLIPWRPPGDRPPNPGELAICLPFLRRLVAIVGPARLLLLGALAAR
                     SLLPQSAARRRPRGAWMDTAEGVPALPTFSPVELMRSAKDRRAAWADLRLLRRTLDAD
                     ASDGDASGG"
     gene            complement(4726..5412)
                     /locus_tag="VKQ27_17545"
     CDS             complement(4726..5412)
                     /locus_tag="VKQ27_17545"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLY90790.1"
                     /translation="MTTLRIGCLIFPRMTQLDLTGPFEVFSRVPDAQVLLLWKTLEPI
                     EADTGLRLLPYATLHDCPPLDIICVPGGPGVNDLMEDEEVLTWLRDRAATARFISSVC
                     TGSLVLGAAGLLRGKRATSHWGARDLLATFGATPTAGRVVRDGNVFTGGGVTAGIDFA
                     LAMVAELAGAPVAQAIQLQIEYAPAPPFDAGTPETASPEVLAAVRARGAPMRQARDAI
                     ASRAAARLSA"
     gene            5559..6560
                     /locus_tag="VKQ27_17550"
     CDS             5559..6560
                     /locus_tag="VKQ27_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLY90791.1"
                     /translation="MPRQGMAEMPKTPRNLPKVRRVEIVVFPRVQLLDVAGPMQVFAT
                     ANEFAGEPAPYDVHVVAKQAGAVATSAGLTVVAEGLASARTGVDTLVVAGGVGVYEAL
                     GDAALVGWVRRRAGVARRVASVCTGAFLLAEAGLLDGRRCTTHWRRCEELARRYPSLR
                     VESDPIFVRDGDVWTSAGVTSGIDLVLAFVEADAGRAAALAVARQLVVFAKRPGGQAQ
                     FSAGLSLSGDADFDALHEWMGRNLDRDLSVPRLAERAGMSERSFARHYRAATGATPSR
                     AVERLRVEAAREALGTTRRSVKDVARRCGFGSEETMRRSFLRLLNVVPRDYRQRFPG"
     gene            complement(6606..7193)
                     /locus_tag="VKQ27_17555"
     CDS             complement(6606..7193)
                     /locus_tag="VKQ27_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90792.1"
                     /translation="MTVDRSGALAAALPILLATTLCLCACAGPSGPSSVDQSQQQLAT
                     LSCRFAGPAFWEIGTELRRHYEDGHGHHVGLWCERNIFGAHWDLRIDGDEPPEARRSM
                     TIGGCFFNEGESNGPIIQEGEGHQYAKIEWTNQDPANEHEKYSFAYDYSTGLVTITAT
                     IPGSPPATTVVHPQESWDKLKELLPDPERSVASSN"
     gene            complement(7667..8866)
                     /locus_tag="VKQ27_17560"
     CDS             complement(7667..8866)
                     /locus_tag="VKQ27_17560"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HLY90793.1"
                     /translation="MSAQIIAPAIPSRATATACGGMIAMAAALGIGRFVYTPILPAMM
                     SGLGLSKSAAGLIASANFLGYLLGALLAAWPNLPGTRRTWLLGSLAISGLTTAAMGLT
                     QAMPAFLLLRFIGGAASAIVLVYASAIVLEHVTGANRPALSAVHFGGVGVGIAVSAVL
                     VTALLQAGEQWSALWWTSGLLSLAATVIVAALLHDSPSSGAPIHRAPTRATNPALLRL
                     IVAYGLFGFGYVITATFLVAIVRGSPTIHALEPIIWVIFGLAAVPSVALWGRLASRLG
                     LTTTFAIASVVEAIGVLASVAWPSAPGAFLAAMLVGGTFMGLTALGLVRARALTSGDP
                     RRTLALMTGAFGLGQIVGPSYAGFTAEHLGSFAVPSATAVSALLLAAFLVGAITETAA
                     GDPAPPR"
     gene            8966..9511
                     /gene="moaB"
                     /locus_tag="VKQ27_17565"
     CDS             8966..9511
                     /gene="moaB"
                     /locus_tag="VKQ27_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HLY90794.1"
                     /translation="MPLDESRPFIAVNIAVLTVSDTRTLADDRSGDALVERIGKSGHR
                     VAARAIEKDDAVAIERHLRGWIADADIDVVVTTGGTGITGRDVTPEAFDRVLEKRIEG
                     FGELFRMLSYQKIGTSTMQSRALGGVAGGTYLFALPGSTGAVKDAWDDILVFQLDNRF
                     RPCNLVELMPRLQEHLKTAAE"
     gene            9552..10256
                     /locus_tag="VKQ27_17570"
     CDS             9552..10256
                     /locus_tag="VKQ27_17570"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90795.1"
                     /translation="MLISHRHRFIYTKTIKTAGTSVEIYFEEACIPPGSDIVRGHGIA
                     ETVTEAGVIGYRGVDPGESRWYNHLPASNIRELAGPEIWDSYFKFCVIRNPFDKLVSL
                     WWAIIGANERYAEIIRDFSSIKAEFASWCVEAAANAVDRDKYLIDGQVAVDDFIRYES
                     LMADLERVCIRIGYPFRPARLGQYKSGLRLSARPSVEYYDELAAQAVYDAFRWELDYF
                     GYTGPSEDCQQAIVDP"
     gene            10357..12516
                     /locus_tag="VKQ27_17575"
     CDS             10357..12516
                     /locus_tag="VKQ27_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HLY90796.1"
                     /translation="MNDAELPITGYLDRFSHRPGETFSAFVSVPGGGRYRARLVRVLS
                     GDPNPAGPGVRFEDLSSRFDQTFEGRRQAIHVGSYGVVKTGPEREAKDACTWTVLVRP
                     GVVDAGQVLFAEGDVALVIGADGVTGRIGATEVATGAPMQAARWYRAWVSADPATGRV
                     LVGQQKLGSAAVTAETLAHGLALPSGGEVTFAASNAAAPTQHFTGKLEDPAILPGFIE
                     GWPDPGATLEALAPECLAAWDFSLGIDSEAIYDIGPQARHGRVVNMPTRAVVGVRWSG
                     SEQCWRHAPHDYGAIHFHVGDLGDCRWQSDLSWTVPDDLKSGAYALHLSCEAGEDWLP
                     LYILPKRQGPFAPIAFLASTFTYQAYANHARAGLDDGYFARVAEWGAYPHNPVRYPLY
                     GRSTYNRHHDGSGISFSSRHRPIMTMRPGFLTFNDPRGSGMRHYPADLHLLAWLEAKG
                     FAFDIVTDEDLDDEGVELIAPYRTVLTGSHPEYHTLGTLDALAAYTQSRGRLCYLGGN
                     GFYWRVARSKARPHLIELRRAEGGIRTWAAEPGEYFHQTDGQLGGLWRRNRRPPQMLG
                     GVGFSCQAAFEGVHYRRLPASREARFAWMFDGIEEDVLGDYGLSGGGAAGFELDRADP
                     ALGTPDGTVILARSEGRLESFTTVPEELLTVTISVNGETVEDLQRGEIVYFETPGGGE
                     VFAVGSITFCGSLWRNGFEGPVSRLLENVVRRFSGDG"
     gene            12509..13324
                     /locus_tag="VKQ27_17580"
     CDS             12509..13324
                     /locus_tag="VKQ27_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLY90797.1"
                     /translation="MADNSAQRRSRSYKLPVEDLDFLLRDEMRDIRFALEYAKPELLL
                     RDWGIRSTIIIFGSARTPSPEQAEAAVTAATTDAERAAARRLVQRSAHYSAARELGRI
                     ISERGGAFSPEHRWRDNVVATGGGPGIMEAANRGAFDAGAPSIGFNITLPKEQQPNQY
                     STPELTFQFHYFAMRKMHLAMRANALVVFPGGFGTMDELFEILTLEQTRKAPGMPIVL
                     FDQAYWRRMINFDALIEEEVIAPEDVALFEFADTAEAAWEVMVRRGLKAHIPP"
     gene            complement(13327..>14169)
                     /locus_tag="VKQ27_17585"
     CDS             complement(13327..>14169)
                     /locus_tag="VKQ27_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLY90798.1"
                     /translation="GMLDAGLTDDQRAWFAARNIRVVRPTPPTRRAAEASAERPALAV
                     NLAKPWLDVLFPGHTTLVWLDADTWVQRWRAVELLAGAAGTGAMAVVADSGRYWNKLL
                     DVRWLAAGIGGFAQIRGFLAKSAWHARLSPALCRKLGDRASPNAGVFALRHDAPHWQR
                     MREWQERVLRHGKPFTSDQLAMALTIHADGYPVELLSTSCNYSTQPRVDPVNVALLEY
                     YYPYDPVGIVHLSAQKAMRIDPSAKTRLLGTDGQVYHANLRYGLFHRAIREAAQRAPE
                     ALPA"
BASE COUNT         2330 a         3976 c         5397 g         2466 t
ORIGIN      
        1 ttcccgcggc atcgtcgcgg gcgtgttctg tgcaagcaat gcagagaagt cctcggcacc
       61 cagacctcgg ccgaacagga agccctgcac ctcctcgcag ccttcctcca tcagcatgtt
      121 tgcctgagct tcctgctcga cgccttccgc attgacgcgg atacccatgg catggctcat
      181 ccgcaggact gcgcgaacga tctcagcggc gtgcgagtcg gtttgcaagg ccgcgacgaa
      241 cgagcgatcg atcttgatct tgtcgaatgg gaacttctgc agatagccga ggctggagta
      301 gccagtgccg aagtcatcca tcgcgatcgt cacgccaatg gcgtgcaaac gacgcagcgt
      361 ggcgagcgtt tcctcggtct cgtgcagcag gacgccctcg gttatctcga gttccagacg
      421 ccgcccatcg attccgacgc gcgccagtgc gttcttcacc tgatcgacga aacccgggcg
      481 acggaactgc actggcgaga cgttgacggc catgcagttc aggctgggcc accgggctgc
      541 ctggcggcac gcctcacgga gcacccactc gccgatgcgg acgatcagac cacaatcctc
      601 cgcgagcggg atgaatgctt ccggccgaac ctcgccgcga actggatggt gccaacgaag
      661 cagtgcctcg gctcccacaa cgcgacgctc ggcaagatcg aactgtggtt gatagtgcag
      721 acggaactga ccctgtgcga gggccgttac aatgtccgat tcgagcgcgc gccgttccaa
      781 cagcttctcg ttcatttcgg tagcgaagaa gcgatagaca ccacgaccct cctctttcga
      841 gcggtacagg gcggtgtcgg cttcctgcag caggatgccg ggatcggcat tggatacggc
      901 gagctctgtc gcgcggcgca acgcgatgcc tacactgacg cccacgttca cctggttgcc
      961 cgccagatcg aatgcctggt ccattgcatc gatcagccgt tgtgccagca tttcgatgtc
     1021 ggcaagctgg cgtatccctg cctgtatgat ggcgaattca tcgccaccca ggcgtgccag
     1081 cgtatcagtt tcgcggatgc atgtgcggag ccgtccagcc acgccaacga gcagtcgatc
     1141 gccggcggcg tggccgagtg tgtcgttcac ctccttgaac cggtcgagat cgagatagag
     1201 tatggccagc atggagccgt cgcgtcggca ccaggccaac tcctgctcca cccgatcatg
     1261 gaacagtgcg cgattgggca agcctgtcag ggtgtcatga gttgccagat gcgttgatcg
     1321 ggccagtgcg cgctgcagca ggcgcagggg ccatgaccgc agcaccgcga aagtgaccgc
     1381 tcccagcgac gccgcgaatg cagcgatcgc cgccgtgaca agccaaagtt gccgctgtga
     1441 gcgctgtatc tctacacgcc ccacgatgtt gccggaatcg tacaagggcg ttcccgccgt
     1501 catcagcggg ggcagcacac tgtcgccttg ctcggccaca agttgatcgc gcccgaagaa
     1561 gacagccctt ctctcggtat cttgcagcgg accgagcatc gccaaaagac cacggatccg
     1621 cgcattttcg aatgcccata gctcgggatt cgtgttgatc aactgggaga tcaccgcgct
     1681 ggccacttta ctctcggcgg caacctcggc gtggcgtgcc gcgctggctg acaggaaata
     1741 ggtcgacggg agcatgagcg tgacggcaag cgcgattgtc gccgcgagcg cggtgacggt
     1801 gtgcgtcagg gccggctcaa ggattcgacg gatcatgcga taccggccag cggaatgtga
     1861 cccagccgcg cgagtatgtc cttagcctgc ggggtggcga ggaaggtgac gaacgcagcg
     1921 gcttcaacac gaggctggtc gcgccagacg acataaagcg tgcgcgatag tttgtagcga
     1981 tttgcagcca gatcctccgt cgtcggcggt tcgccatcca gcgtgagcgg gacgacgttg
     2041 cgtcgctcgg cacggatctg tccgagcgac atcgctccga aagcgcccgg caggcgttcg
     2101 aggacgtcgg cattctcctg atcggtagat acggtgagca gtcccggccg atccagcgcg
     2161 atcttgatcg cgtcggtcat gtccggcgac gcagcacgca gcatgctcca gtcggcatcc
     2221 gatggttccc gtcgaatgag gcgtatcgcc ttgccgtttg gccagttgcg caactgtcca
     2281 cgaaaaatct gcgcgacctg tgcgagcgtg atctcgcgga tgccggcgtc ggggtgtgcg
     2341 gcgaacgcga tgggtgtgcg cgcatatggc atggcttgca gccccttgga gcgttcggcg
     2401 tcgttcagag cgcgcgcagc cagcgcgagt tcgattgccc cggcggccac cgcgttgaga
     2461 ccaccgccag tgccgagact gggcaataca ttcaccgcga catcgggatt tgttgcggta
     2521 aaggcggtgc cgatttcgcg tatgggggcg agcgccattc ctgtgccgcc aacgcgcaat
     2581 tgcgtgcctg ccgcggcagg cgtcatgcgc gcgaacagtg gtgcggcagc aagaacggca
     2641 gcgacggccc gggtgaacag ggcgcgacgc tgcagtgcgc ccgcgacggc agcacggcgt
     2701 gacagagggc cgatgttccg catgcctctg ctcccaggga acgagaacat ccccaggtct
     2761 gacgtaaaat tctcaaaaca gggtaaaagc cggacggcag atgcgatgac gccggtgggg
     2821 cggtcgatcg agaaggctgc cagcggccga cgcaacagag ccaacgatcc gcctcttggc
     2881 gaattccgct gcgcgtgtct ccattgaggc cagtgcaagg aaaaggagcc gacaccatgt
     2941 ccgcgaccgc cgcaatgtgg cctgctggcg ctgcctatat cgacgggcgc ttcatgccga
     3001 tcgccgaggc gatgattccg gtgaccgaat gggggtatcg ccggtcggat gtgacctacg
     3061 acgtggtcag cgtgtgggac ggcgccttct tcaagctggc ggaccacctg aagcggttcc
     3121 gtacgtcgat ggagcattac cggctgcagc cgaaggagag cgacgacgat atccgcgacg
     3181 tgctgcatga actggtgcgg cggacggggc tgcgcgaggc gtatgtcgcg atggactgcc
     3241 tgcgcggttc ggctccggcg catctgcggc ggcatccttc caacgcgcgg aactatctgc
     3301 tggcgttcgc cacgccttac gtgtggctga tgccgaagga tgtgatcgag cggggggcgc
     3361 atctgatcgt ggcgtcgacg ccgcggattc cggaggcgtg cgtcaatcct cgggcgaaga
     3421 acttccattg ggccgatatg acgaaggcgc tgttcgaggc gcaggacaag ggcgcggaca
     3481 atccggtgct gctcgacctg gatggaaatg tgaccgaggg gccggggttc aatgtgttcg
     3541 cggtgacgga tggcgtggtg gcgacgccgg atcgcggggt gctggagggg atcacgcggc
     3601 tctcggtgat ccagatgtgc gagaagctgg gagtgaagtg cgaggtgcgg accgtgccgc
     3661 tgcaggaact gcggaatgcg gacgaggtgt tcatttcgtc aacagccggt ggggtgatgg
     3721 gggtaacgcg gctggatgag cggacctttg gcaatgggcg gccggggccg gtgacggagc
     3781 ggctgcacga gtcttactgg cggttccggc gcgagggatg ggactgcgag ccggtcgact
     3841 acgtgatgcg ggcggaggcg gccgagtaag gcgcagacag tggatgatct ggccgcgctc
     3901 cggctgcaga tcgagtgggg ggcggacgag gcgctggaag atgcgcctgt ggatcgggtg
     3961 catagggtgc aggtgcatgc tcctgcggca ctcgctcccc cgccccggat ccagtccggg
     4021 gcagtgtcct cacccgcgcc aagcgtggaa gagggggtgc ggcgtgccct ctcgcgcgga
     4081 gtgggcgagc ttggggatac ggcggtgccc agccgcgccg gtgcggccga gcgggcgctg
     4141 caggtggcgg cgggggcgtc cacgctggag gagttgcggg cggccgttgc tgcgttcgat
     4201 gggtgcgcac tgcgcgatac ggcgagtcac ctggtgttcg cggcagggga tccagcggcg
     4261 tcgctgctgg tgattgggga accgccgggg gcggatgacg atcggtcggg cgcgccgttc
     4321 gccggggcgg ctggggcgta tctggacagg atgctggggt cgatcggatt ggcgcgggac
     4381 gcactgctgc tgacgccgct gatcccgtgg cggccgccgg gggatcggcc gcccaatcca
     4441 ggggaactgg cgatctgcct gccgttcctg cggcggctgg tggcgattgt ggggcccgcg
     4501 cggctgctgc tgctgggggc gctggcggcg cgatcgctgt tgccgcagag cgcggcgcga
     4561 cgacgaccgc ggggggcgtg gatggatacc gcggagggcg tgccggcgct gccgacgttc
     4621 agcccggtgg agctgatgcg ctcggccaag gaccgccgcg ctgcgtgggc agatctgcgg
     4681 ctgctgcggc gaaccttgga cgcggacgct tcagatgggg acgcctcagg cggataggcg
     4741 ggcggcggca cgcgaggcga tggcgtcgcg ggcctggcgc atgggggcgc ctcgggcgcg
     4801 gacggcggcg aggacttcgg gcgaagcggt ctcgggcgtg ccggcgtcga agggcggcgc
     4861 gggggcgtat tcgatctgga gctggatcgc ctgggctaca ggtgcgccag cgagctcggc
     4921 caccatggcg agggcgaagt cgatcccggc ggtgacgccg ccgccggtga acacgttgcc
     4981 gtcgcgcacc acgcggcctg cggtgggtgt ggcgccgaac gtggcgagca ggtcgcgagc
     5041 accccagtgc gaggtggcgc gcttgccgcg cagcaggccg gcggccccga gcacgagaga
     5101 gccggtgcag acggagctga tgaagcgggc ggtggcggcg cggtcgcgaa gccaggtcag
     5161 gacttcctcg tcttccatca ggtcgttcac gccaggaccg ccggggacgc agatgatgtc
     5221 gagcggcggg cagtcgtgga gcgtggcgta ggggagcagg cggaggccgg tgtccgcctc
     5281 gatcggttcc agcgttttcc agagcagcag gacctgggcg tcggggacgc gggagaagac
     5341 ctcgaagggg ccggtgaggt cgagctgtgt catgcgcggg aagatcaggc agccgatgcg
     5401 gagcgtggtc atggcagtgg gtccttgttc ttgcgagatg aaagccaccg ctgcggcggc
     5461 ccccacctct cctctccgtc cctggggacg gagagggatc tgcttcgccg ggtcaccagc
     5521 ggcgggatgt gtgaataccg cagctggaca gggacatcat gccgcggcag ggtatggcgg
     5581 aaatgccaaa gacccctcga aatctgccaa aggtgcgccg cgtggagatc gtggtgtttc
     5641 cgcgcgtgca attgctggac gtggcggggc cgatgcaggt gttcgccaca gcgaacgagt
     5701 tcgcgggaga gccggcgcca tacgatgtgc atgtggtggc gaagcaggcg ggcgctgtcg
     5761 ctacatcggc ggggttgaca gtggtggcgg aggggttggc ttcggcgcgg actggtgtcg
     5821 atacgttggt agtggcgggc ggcgtgggtg tgtatgaggc gctgggcgac gcggcgctgg
     5881 tggggtgggt ccggcggcgg gctggggtgg cgcggcgggt ggcgtctgtg tgcacggggg
     5941 cgtttctgct ggcggaggcg gggctgctgg atgggcggcg ctgcacgacg cattggcggc
     6001 gatgcgagga actggcgcgg cgatatccgt cgttgcgcgt ggagagcgat ccgatctttg
     6061 tgcgcgatgg ggatgtgtgg acgtcggcgg gcgtgacgtc ggggatcgat cttgtgctgg
     6121 cgtttgtgga ggccgatgcg gggcgtgcgg cggcgctggc tgtggctcgg cagttggtgg
     6181 tgttcgccaa gcggccgggc gggcaggcgc agttcagcgc gggtctgtca ctgtcaggcg
     6241 atgcggattt cgacgcgctg catgagtgga tggggcggaa tttggatcgc gatctgtcgg
     6301 tgcctcggct agcggagcgc gcggggatga gcgagcgcag ctttgcccgg cattaccgtg
     6361 cggcgaccgg ggcgacgccg tcgcgggcgg tggagcggct gcgggtggag gcggcgcggg
     6421 aggcgctggg gacgacgcgg cgctcggtca aggacgtggc gcggcggtgc gggttcggct
     6481 cggaagagac gatgcggcgg agtttcctgc ggctgctgaa tgtggtgccg cgggattacc
     6541 ggcagcggtt tccggggtag ggggtgttcg gaggttgggg ggtcgttcgc gcggcgcttc
     6601 atcaactaat ttgatgacgc aacggaacgc tctggatcag gcagcagctc tttcagtttg
     6661 tcccaacttt cctgggggtg aaccactgtc gtggctggcg gcgagcctgg gatggtggcg
     6721 gtgatcgtca cgagccccgt gctgtaatca taagcgaagc tgtatttctc gtgctcattt
     6781 gcaggatctt gattggtcca ttcaatcttg gcgtattggt gaccttctcc ttcttgtatg
     6841 atcgggccat tgctctcgcc ctcattgaag aagcacccgc cgattgtcat tgagcggcga
     6901 gcttccggag gctcatcacc atcgatgcgc aggtcccagt gggcaccgaa aatattccgt
     6961 tcacaccaca atcccacgtg gtgcccatga ccatcctcat aatgtcgccg caactcggtg
     7021 ccgatttccc agaatgcggg cccggcaaat ctgcacgaca acgtcgccaa ctgctgctgg
     7081 ctctggtcca ccgacgacgg gccagacggg ccagcgcaag cacacaggca gagtgtcgtg
     7141 gcgagcagga tgggcagtgc cgccgcaagc gcgccgcttc tgtcgacggt cacggccgca
     7201 cgccacttag gcacgaaggt cgaggttcca acgccaaata gactggcgcg gagcgatcgg
     7261 ctcgttgatc atgaggctgc ccgttagagc ggcatgtgga atgaggcgga cttgatgtca
     7321 ctcgcatgtc gaacaggatt atgggcttgg cgcgatccgc tggccatccc gccacatcgt
     7381 tccccttccg cactgcctgt ctcgatagcg tcaagtgcca cccgcgatta ccggcagcgg
     7441 tttcaggggt aacgggtgtt tggaagcgta cggcgttcgg ggtgcacttg cagggcgagg
     7501 atggggcgcg aaggggaggc gtgaggccaa cgttgaccaa ctagctcatc aggtcagctt
     7561 tctcctggtt cggcgtggct gaggtggcgg tggggcatgc tcgctccact gcggttggct
     7621 tagccgattg gaagccggag ggcactcggt cccaggtctc ggctgctcag cgtggtggcg
     7681 cgggatcacc ggcagcggtt tccgtgatag cgccaacgag gaaagccgcg agcaacaggg
     7741 cggagaccgc ggtggcggac gggacggcga agctgccgag gtgttcggcg gtgaatcctg
     7801 catagctggg gccgacgatc tggccgaggc cgaaggcgcc ggtcatcagc gcgagggtgc
     7861 ggcgcgggtc accggatgtc agggcgcggg cacggacgag gccgagtgcg gtgaggccca
     7921 tgaaggtgcc gccgacgagc atcgcggcga ggaaagcgcc gggtgcgctg ggccaggcga
     7981 cgctggcgag gacgccgatg gcctcgacga cgctggcgat ggcgaaggtg gtggtgaggc
     8041 caaggcgtga tgcgaggcga ccccagaggg cgaccgatgg gacggcggcg aggccgaaga
     8101 tgacccagat gatcggttcg agggcgtgaa tggtggggga gccgcgcacg atcgcgacca
     8161 ggaacgtggc ggtgatcacg tagccgaagc cgaacaggcc gtaggcgacg atgaggcgga
     8221 gcaaggcggg attcgtggca cgggttgggg cgcgatggat tggcgcgccg gaggatggcg
     8281 agtcgtgtag caaggcggcc acgatcacgg tggctgcaag cgacaggagg ccgctggtcc
     8341 accacagcgc gctccactgt tctccggcct ggagcagggc ggtgacgagc acggctgaca
     8401 cggcgatgcc gacgccgacg ccgccgaaat gcaccgcgga cagcgcgggg cggttggcgc
     8461 ctgtgacgtg ctcgaggacg atggcggagg cgtagaccag cacgatggcg ctggcggcgc
     8521 cgccgatgaa gcgcagcagg aggaaggcgg gcatggcctg ggtcaggccc atggctgccg
     8581 tggtcaggcc gctgatggcc agggagccga gcagccaggt gcggcgggtg ccaggcaggt
     8641 tgggccacgc ggcgagcagg gcgccgagca gatacccgag aaaattggcg gaggcgatca
     8701 ggccggcggc tgacttgctg aggccaaggc cgctcatcat cgcgggcagg atcggcgtat
     8761 agacgaagcg gccgatgccg agtgcggccg ccatcgcgat catgccgcca caggcagtgg
     8821 cggtggcgcg ggagggaatg gccggggcaa tgatttgggc ggacatgcga acccatctat
     8881 gctctgcacg tgggctttgc tcatggtttg atctgatcgg tccgttcggt ttcgcagatg
     8941 gctcggaccg gggaaggagg cgaacatgcc gctcgacgaa tcccgtccgt tcatcgcggt
     9001 gaatattgcg gtgctgaccg tgtcggatac acgcacgctg gcagacgaca ggtcgggcga
     9061 tgcgctggtg gagcgtatcg gcaagtcagg gcatcgcgtg gcagcacggg cgatcgagaa
     9121 ggacgacgcg gtggcgatcg agcggcatct gcgcggctgg atcgccgatg cggacatcga
     9181 tgtggtggtc accacgggcg gcaccgggat caccgggcgg gacgtgacgc cggaggcgtt
     9241 cgaccgggtg ctggagaagc ggatcgaggg gttcggcgag ctgttccgaa tgctgagcta
     9301 ccagaagatc ggcacgtcga cgatgcagtc gcgggcgctg ggcggcgtgg ccggggggac
     9361 gtacctgttc gcgctgccgg ggagcacggg ggcggtgaag gatgcgtggg acgacatcct
     9421 ggtgttccag ctcgataacc ggttccggcc gtgcaacctg gtggagctga tgcctcgatt
     9481 gcaggagcat ctgaagaccg cggcggagtg acggttcgcg ccttgccggt cgcggtcgcc
     9541 aggggcgcga catgctgatc agtcatcgcc accggttcat ttacaccaag accatcaaga
     9601 cggctggaac gtcggtcgag atctacttcg aagaggcgtg cataccgccc ggcagcgata
     9661 tcgtgcgcgg ccatggcatt gccgagaccg ttaccgaagc gggcgtgatc ggctaccgtg
     9721 gcgtggatcc gggtgagagc cgttggtaca accacctgcc ggcgagcaat atccgcgagt
     9781 tggcggggcc ggagatctgg gacagctatt tcaagttctg cgtgatacgc aatccattcg
     9841 acaagctggt gtcgctctgg tgggcgatca tcggcgccaa cgagcgttac gctgagatca
     9901 tcagggactt ttcaagcatc aaggcggagt ttgccagttg gtgtgtcgag gcggctgcca
     9961 atgccgttga ccgggacaag tatttgatcg atggccaggt cgcggttgat gatttcatcc
    10021 gatacgaatc attgatggct gatctggaac gggtgtgtat ccggatcggc tatcccttcc
    10081 ggccggctcg gcttgggcag tacaagagcg gtctccgcct gagtgcccgt ccatcggtgg
    10141 aatattacga tgaactggcc gcacaggcgg tctatgacgc gtttcgctgg gaactggatt
    10201 atttcggtta caccgggcct tcggaggact gccagcaggc gatcgtcgat ccataaggtc
    10261 gccggggcgt gcggtcgggt ctggtatatt gacattgcag cggcggcatc acgtcatggt
    10321 ccggagctat gacgtttggg agctgtgtat ctacaaatga acgacgccga actgccgatc
    10381 accgggtatc tcgaccgatt ctcgcatcgt cctggcgaga cgttttcagc gtttgtcagt
    10441 gtgcctggtg gagggcggta tcgcgcgcgg ctggtgcgcg tgctgagcgg cgatccaaat
    10501 ccagcgggac caggcgtgcg gttcgaggat ctgtcgtcgc gcttcgacca gacgttcgag
    10561 gggcggcggc aggcgattca tgtcggctcg tatggcgtgg tgaagaccgg gcccgagcgg
    10621 gaggcgaagg acgcgtgcac ttggacggtg ctggtgcggc cgggcgtggt cgacgcgggg
    10681 caggtgctgt ttgctgaagg cgatgtggcg ctggtgatcg gggcggatgg cgtgaccggg
    10741 cggatcggtg cgaccgaggt tgcgaccggc gcgccgatgc aggcggcgcg atggtatcgc
    10801 gcatgggttt ctgccgatcc cgcgacgggt cgtgtgctgg tgggtcagca gaagctgggc
    10861 agcgcggcgg tgacggccga gacgctggcg catgggctgg cgctgccgtc cggcggcgag
    10921 gtgacgttcg cggcgtcgaa cgctgcggcg ccgacgcagc atttcaccgg caagctggaa
    10981 gatccggcga tactgcctgg ttttatcgaa gggtggccgg atcctggtgc gacgttggag
    11041 gcgttggcgc cggaatgcct cgcggcgtgg gatttctcgc tggggatcga tagcgaggcg
    11101 atctacgaca tcggaccgca ggcgcgccat ggcagggtcg tcaacatgcc gacacgtgcc
    11161 gtggttggcg tgcgctggag cggaagcgag cagtgctggc gtcatgcgcc gcatgactat
    11221 ggcgcgatcc atttccatgt cggcgatctg ggggattgcc gctggcagtc ggatctttca
    11281 tggacggtgc cggatgacct gaagagcggc gcctatgcac tgcatctgag ctgcgaggcg
    11341 ggcgaggact ggctgccgct atatatactg ccgaagcggc agggaccatt cgcgccgatc
    11401 gcgttcctgg cttcgacgtt cacgtatcag gcgtatgcca atcatgcgcg ggcgggactc
    11461 gatgacgggt atttcgcacg cgtcgcggag tggggggcgt atccacacaa tccggtgcgg
    11521 tacccgctgt atggacggtc aacctataac aggcatcatg acgggagtgg catttcgttc
    11581 tcgtcgcggc atcggccgat catgacgatg cggcctgggt ttctgacgtt caatgatcct
    11641 cgggggtcgg ggatgcggca ttatccggca gacctgcatc tgctggcgtg gctggaggcg
    11701 aaggggttcg ccttcgatat cgtgaccgat gaggatctgg acgatgaggg cgtggaactg
    11761 atcgcgccgt accgcactgt gctgaccggg tcgcatccgg agtaccacac gctggggacg
    11821 ctcgatgcgc tggccgcgta tacacagagt cggggacggc tgtgttatct cggcggcaac
    11881 gggttctact ggcgcgtggc gcggagcaag gcgcggccgc atctgatcga gctgcggcgg
    11941 gcggaagggg gcatccgcac ctgggcggcg gaaccgggcg agtactttca ccagacggat
    12001 gggcagctgg gtgggctgtg gcgtcgcaac cggcggccgc cgcagatgct ggggggcgtt
    12061 gggttctcgt gccaggcggc gttcgagggg gtgcattacc ggcggctgcc tgcgtcgcgc
    12121 gaggcgcggt tcgcgtggat gttcgatggg atcgaggagg atgtgctcgg cgattacggg
    12181 ctgtcgggcg gcggggcggc ggggttcgag ctggatcgcg cggatccggc gctggggacg
    12241 ccggacggga cggtgatcct ggcgcgatcg gaggggcggc tggagtcgtt cacgacggtg
    12301 ccggaggaac tcctgaccgt cacgatcagc gtcaatggcg agacggtgga agatctgcag
    12361 cgcggggaga tcgtgtattt cgagacgccg ggcggtggcg aggtgttcgc ggtggggtcg
    12421 atcacattct gcgggagttt gtggcggaac gggttcgagg ggcctgtgtc gcggctgttg
    12481 gaaaacgtgg tgcggcggtt ctcgggcgat ggctgacaac agcgcgcagc ggcggtcgcg
    12541 tagctacaag ctaccggtcg aggacctgga tttcctgttg cgcgatgaga tgcgcgatat
    12601 ccggttcgcg ctggaatatg cgaagcccga gttgttgctg cgggactggg gcatccggtc
    12661 cacgatcatc atattcggca gtgcgcgtac gccatcgccg gagcaggcgg aagcagcggt
    12721 tacagcagcg acgacagatg cggagcgcgc ggcggcgcgg cggttggtgc agcgttcggc
    12781 gcattacagc gcggcgcgcg agctgggccg gatcatttcg gaacgtggcg gggcgttctc
    12841 gccggagcac cggtggcgcg acaacgtcgt ggcgacgggc ggcgggccgg ggatcatgga
    12901 ggcggcgaac cgtggcgcat tcgatgccgg ggcaccgagc atcggcttca acatcacgct
    12961 gccgaaggag cagcagccga accagtacag cacgcccgaa ctgaccttcc agttccacta
    13021 cttcgcgatg cgcaagatgc accttgcgat gcgggcgaat gcattggtgg tgtttccggg
    13081 cggctttggc acgatggacg agctgttcga gatcctgacg ctggagcaga cgcgcaaggc
    13141 gccggggatg ccgatcgtgc tgttcgatca ggcttactgg cggcggatga tcaatttcga
    13201 tgcgctgatc gaggaggaag tgatcgcgcc ggaagacgtg gcactgttcg agttcgccga
    13261 tacggcggag gcggcgtggg aggttatggt gcggcgtggg ctcaaggcgc atataccgcc
    13321 gtaaggtcag gcgggcaggg cctcaggcgc gcgctgcgcc gcttcgcgga tggcgcggtg
    13381 gaacaggccg tagcgcaggt tcgcgtggta gacctgcccg tctgtgccga gcagacgggt
    13441 cttcgccgag gggtcgatgc gcatcgcttt ctgggcgctg aggtgcacga tgccgacggg
    13501 atcgtatggg tagtagtatt cgagaagggc gacgttgacg ggatcgaccc gcggctgggt
    13561 gctgtagttg cacgaggtcg atagcagttc gacagggtag ccgtcggcat ggatggtgag
    13621 cgccatggcg agctggtccg aggtgaaggg cttgccgtgg cgcagcaccc gctcttgcca
    13681 ctcacgcatg cgttgccagt gaggtgcgtc gtggcgcagg gcgaagacgc cggcatttgg
    13741 cgacgcgcgg tcacccagct tgcggcacag cgcgggcgac aggcgggcgt gccaggcgct
    13801 cttggcgagg aagccgcgga tctgggcgaa gccgccgatg ccggcggcga gccagcgcac
    13861 gtcgaggagt ttgttccagt agcgccccga atcggcgacg acggccatcg ctccggtgcc
    13921 ggcggcgccg gcaagcagct ccacggcgcg ccagcgctgc acccaggtgt cggcgtcgag
    13981 ccagaccaga gtggtgtggc cggggaacag gacgtccagc cagggcttgg cgagattgac
    14041 ggcgagcgcc gggcgttccg cactggcctc ggccgcgcgc ctggttggcg gtgtggggcg
    14101 aacgacccga atgttgcgcg cggcgaacca ggcacgctgg tcatcggtca ggccggcgtc
    14161 cagcatgcc
//
LOCUS       DATGEM010000575         4945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_664028, whole genome shotgun sequence.
ACCESSION   DATGEM010000575 DATGEM010000000
VERSION     DATGEM010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664028"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VKQ27_17590"
     CDS             <1..638
                     /locus_tag="VKQ27_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLY90799.1"
                     /translation="MAAVANIEITITGKGAHGAMPHFGIDPIVIASHIVTSLQSIVAR
                     NVEPLESGVVTIGHINGGHTYNVIPETVHMLGTARWFDPTVGDQLENGVRRLATGIAE
                     SFGAKADVVFSRDYPATVNDPEATTLTRRAATAVAGDTRVEHMAKPTMGGEDFSFMLN
                     AKQGSYIMLGGGRGKDDAMVHHPRYDFNDEILPVGASYWATLVEQLLPRKD"
     gene            complement(737..1402)
                     /locus_tag="VKQ27_17595"
     CDS             complement(737..1402)
                     /locus_tag="VKQ27_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLY90800.1"
                     /translation="MFYHWDFALILRYAPLFWKGVLVTLAYTAGSIFLGLIIGLLIGM
                     GRLSRSKLLNIPLIGFIEAFRCTPLLVQIVWFYYALPVLLGIQIPATVAATLVLSCYT
                     GAFYAEIFRGGIISIERGQWDAARALGLKPWHLMRKVILPQAIKRMIPPFVNQSITQL
                     KNTSLVSTIAVPDLLYQGTLVTGDTYRPLEVYTVVALIYFAMLFPSTLLAQAYERSLA
                     KGR"
     gene            complement(1421..2266)
                     /locus_tag="VKQ27_17600"
     CDS             complement(1421..2266)
                     /locus_tag="VKQ27_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLY90801.1"
                     /translation="MTTRLGRRSLAGAAAVLPAATLIGSAQAQSANEVTFDRVRRTKV
                     LRIAALPGELPYFQKDLATGEWSGACIDMASDIAKVFDAKLEYVESTYGNSVLDLQSN
                     KVDLAFALNPTPQRALAIGFCHAAIVHPIGAVVRKGLDPATWDDINKPDVRIVYDIGS
                     LHEVVAKRYCPKAQLTGFKSRDDCTLAMASGRADVHVLAAILGLAALGKNPQLGSYHL
                     LSNPLVALPSCIAMQREPDTRFMDVINAWIDFNRGTSQVREWLLVALGKFGVSREQVP
                     ASLSF"
     gene            2378..2830
                     /locus_tag="VKQ27_17605"
     CDS             2378..2830
                     /locus_tag="VKQ27_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY90802.1"
                     /translation="MSIPSPMTAGVTQQGSSVDGIAWHILGQTYTPLHVTESSFSWHA
                     LLPPGTFVPPHIHPMQDEFIYIFEGRFDLVLDGQSANAGAGDLIRLPRGIPHGLFNNS
                     QSNITCLFWVAPTRMLWDLFKAIDNVPDPAEVVRLAAQHEVNFLPPPG"
     gene            complement(2921..4189)
                     /locus_tag="VKQ27_17610"
     CDS             complement(2921..4189)
                     /locus_tag="VKQ27_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HLY90803.1"
                     /translation="MRILACGMHVLIIGGGIGGLATALSLHDAGIGCTVFEHVRELRE
                     LGVGINTLPHAIKELAALGLLPALDRAGIRTRELIYANRFGQVVWRELRGTDAGLDTP
                     QFSIHRGKLHTVLLDAVRERLGPEAVRTGCRLASFVERDDRVVARFERRDAADAFEVA
                     GDALVGADGIHSAVRAAFYPDEGPPVWNGMMLWRGAGDWPVHVDGRTMVISGGMGQKF
                     VFYPIHADPATPERRLTNWAVMARLGDGSGLPPRREDWNREGRLEDVEPFVSDRFRLE
                     FVDPMALIRASGTVYEYPMCDRDPLPQWSFGRATLLGDAAHPMYPVGSNGASQAVLDA
                     RALARWLAAGDVVAGLAGYDAERRPMTSEIVLNNRKGGPERVIDVMEERAPDGFSDVE
                     AVASYAEREAIVRGYASMAGFAKEQVNAPR"
     gene            complement(4257..4490)
                     /locus_tag="VKQ27_17615"
     CDS             complement(4257..4490)
                     /locus_tag="VKQ27_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90804.1"
                     /translation="MDENLPLAGVIPPSLVRGLLKFTKVKAAFTIASSLFCVLHWLIS
                     HRFSLYVIPGALVVGAAAATLTWWFSNAAMSKG"
     gene            complement(4587..>4945)
                     /locus_tag="VKQ27_17620"
     CDS             complement(4587..>4945)
                     /locus_tag="VKQ27_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90805.1"
                     /translation="APDPTVQMSEDGGKVGGAEAGSDGVEVGSGGALADGVDEVAAVD
                     EEDADGVEEVLDVGGALLLWWIWRKGGWCVWFHADGITNGIWLGRQGKYSGNDTVGDV
                     AEALVEKTWVAGLRRP"
BASE COUNT          987 a         1880 c         1301 g          777 t
ORIGIN      
        1 tcatggccgc cgtcgccaac atcgagatca ccatcaccgg caagggcgcc cacggcgcga
       61 tgccgcattt cggcatcgat ccgatcgtga tcgcgagcca catcgtcacc tcgttgcaga
      121 gcatcgttgc gcgcaacgtc gaaccgctcg agagcggcgt cgtcaccatc ggccacatca
      181 acggcggcca cacctacaac gtgatccccg aaaccgtgca catgctcggc accgcccgct
      241 ggttcgatcc caccgtcggc gaccagctcg aaaacggcgt ccgccgcctc gccaccggca
      301 tcgcggaaag cttcggcgcc aaggccgacg tcgtgttcag ccgcgactac cccgccaccg
      361 tgaacgaccc cgaggccacc acgctgaccc gccgcgccgc caccgccgtc gccggcgaca
      421 cccgcgtcga acacatggca aagcccacaa tgggcggcga ggatttctcg ttcatgctga
      481 acgccaagca gggcagctac atcatgctgg gcggcggccg cggcaaggac gacgccatgg
      541 tccaccaccc ccgctacgac ttcaacgacg agatcctgcc cgtcggcgcc tcttactggg
      601 ccacgctggt cgaacagttg ctgccgcgca aggactagaa gcagggtcag gggagcgctg
      661 ctcccctgca agtaacaggt tctaagggcc acgcccttag cggggatcca agacgcaggc
      721 cccttggcct tacgtcctag cgtcccttcg ctaggctgcg ctcgtacgcc tgcgccaata
      781 gcgtgctggg gaacagcatc gcaaagtaga tcagcgccac aactgtatag acctccagcg
      841 gccgatacgt atctccagtc accagcgtcc cctgataaag caggtcgggc accgcgattg
      901 tcgacaccag cgacgtgttc ttgagctgcg tgatcgactg gttcacgaac ggcgggatca
      961 tccgcttgat cgcctgcggc agtatcacct tgcgcatcaa gtgccacggc ttcagcccca
     1021 gcgcgcgcgc cgcatcccac tgcccacgct cgatcgatat gatcccgccg cgaaagatct
     1081 ccgcgtagaa tgcgcccgtg tagcacgaca gcaccaaagt cgccgccaca gtcgccggaa
     1141 tctggatccc cagcagcacc ggcagcgcat agtagaacca cacgatctgc accagcagcg
     1201 gcgtgcagcg gaacgcctcg ataaagccga tcagcggaat gttcagcagc ttcgagcgcg
     1261 aaagccggcc catcccgatc agcagcccga tgatcaggcc caggaaaatc gagcccgccg
     1321 tataggcgag cgtgaccaac acgcccttcc agaacagcgg cgcatagcgg aggatcaggg
     1381 caaagtccca gtgatagaac atccgccccg acccgccgcg tcagaagctc aacgacgccg
     1441 gcacctgttc gcgcgacacg ccgaacttgc ccaaggccac cagcagccac tcgcgcacct
     1501 gcgacgtccc gcgattgaaa tcgatccacg cattgatcac gtccatgaac cgcgtgtctg
     1561 gctcgcgctg catcgcgatg cagctcggca gtgccaccag cggattggac agcaggtgat
     1621 acgaaccgag ctgcgggttc ttgcccagcg cggccagccc caggatcgcc gccagcacat
     1681 gcacatcggc acggcctgac gccatcgcca gcgtgcagtc atcgcgcgac ttgaagcccg
     1741 tcaactgcgc cttcgggcaa tagcgcttcg ccaccacctc atgcagcgag ccgatgtcgt
     1801 agacaatacg cacgtccggc ttgttgatgt cgtcccaggt cgccggatcg agccccttcc
     1861 gcaccaccgc cccgatcgga tgcacgatcg ccgcatgaca gaaaccaatg gccagcgcgc
     1921 gctgcggcgt cggattcaat gcgaacgcca ggtcaacctt gttcgactgc aggtcgagca
     1981 ccgaattccc ataggtggac tcgacatact ccagcttcgc gtcgaacacc ttggcgatgt
     2041 cactcgccat gtcgatacac gcgccactcc actcgcccgt cgccagatcc ttctggaaat
     2101 acggcagctc gccaggcaac gcagcgatgc gcagaacctt ggtgcggcgg acccggtcga
     2161 aggtgacttc gttcgcggac tgcgcctgcg ccgaaccgat caacgtggca gccggcagca
     2221 ccgcggccgc accggccaac gaccggcggc cgagtctggt ggtcatggtt ttatgtctcc
     2281 caggttggcg ccccgatcct gtttcggttg acgcggactt acaaggccac cgaccgcatt
     2341 ctatgctgat ttccgccgaa ccacagggaa gcacaccatg tccatcccgt caccgatgac
     2401 cgccggcgtc acgcagcaag gcagcagtgt cgacggcatc gcctggcaca tcctcggtca
     2461 gacctacacg ccgctccacg tgacggagag cagcttctcc tggcatgccc tgctgccacc
     2521 cggcactttc gttccgccgc acatccaccc gatgcaggac gaattcatct acatattcga
     2581 gggcaggttc gatctcgtgc tcgacggcca gtccgccaac gccggcgccg gcgacctgat
     2641 ccgtctgccg cgcggcatcc cgcacggcct gttcaacaac tcgcagtcga acatcacttg
     2701 cctgttctgg gtcgcgccca cgcgcatgct gtgggatctg ttcaaggcga tcgacaacgt
     2761 ccccgacccc gccgaagtcg ttcgcctcgc cgcccagcac gaagtgaact tcttgccgcc
     2821 acccggctga tcagaacccg gctcacctgc cccacaccct tgtcattgcg acaccggcga
     2881 aggccggtcg aagcaatctt gtggagtttt ccagcccgtc tcaccgcggc gcgttcacct
     2941 gctccttggc gaaccccgcc atcgacgcgt aaccccttac gatcgcctcg cgttccgcat
     3001 acgacgcgac cgcctccaca tcgctgaacc catccggcgc gcgctcctcc atcacgtcga
     3061 tcacccgctc cggcccaccc ttgcgattgt tcagcacgat ctccgacgtc atcggccggc
     3121 gttccgcatc gtacccggcc agccccgcca ccacatcgcc cgcggccaac caccgcgcca
     3181 gtgcacgcgc atcaagcacc gcctggcttg caccgttcga tcccaccgga tacatcggat
     3241 gcgccgcatc cccgagcaac gtcgcacgcc cgaatgacca ctgcggcaac ggatcgcgat
     3301 cgcacatcgg atactcgtag accgtgcccg acgccctgat cagagccatc ggatctacga
     3361 actccagccg gaaccgatcg ctcacgaacg gctccacatc ctcgagccgt ccctcccggt
     3421 tccaatcctc ccgccgaggc ggcagcccgc tcccatcccc caaccgagcc atcaccgccc
     3481 agttggtcaa ccgccgttcc ggcgtcgccg ggtccgcatg gatcggatag aaaacgaatt
     3541 tctggcccat ccctcccgag atcaccatcg tccgcccatc gacatgcacc ggccagtccc
     3601 cggccccgcg ccacagcatc atgccgttcc acactggcgg gccttcatcc ggatagaacg
     3661 ccgcccgcac cgctgaatgg atgccgtcgg cgccaaccag cgcatccccc gccacctcga
     3721 acgcatcggc cgcatcgcgc cgctcgaacc gcgccaccac ccgatcatct cgctcaacaa
     3781 acgacgccag ccggcacccc gtgcgcaccg cctccggccc cagccgctcg cgcaccgcat
     3841 ccagcagcac cgtatgcagc ttgccgcgat ggatggagaa ctgcggcgta tcgagcccgg
     3901 catcggtgcc ccgcagttcc cgccacacca cctggccgaa gcggttcgca tagatcagct
     3961 cgcgcgtccg gatccccgcg cgatcgagcg ccggcagcaa ccccagcgcc gccagctcct
     4021 tgatcgcatg cggcagcgtg ttgatgccca caccaagttc gcgcaactcg cgcacatgct
     4081 cgaacacggt acacccgatg ccggcgtcat gcagcgacag cgccgtcgcc agcccgccaa
     4141 tcccgccgcc gatgatcagg acatgcatgc cgcaagctaa tatgcgcacg cgctatctgc
     4201 caagaatgcc acacatgaag gccgaccaac caagggtcca agtgcgttcg gtatcatcat
     4261 cccttgctca tcgcagcatt ggagaaccac catgtcagcg tcgcggccgc ggcgccgaca
     4321 accagcgcac ctgggataac atacaacgag aaccgatggc tgatcagcca atgcagaaca
     4381 cagaacaggg aagaggcgat cgtgaaggcg gccttcacct tggtaaactt caacagcccg
     4441 cgcaccaggc tgggcgggat cactcccgcc aacggcaggt tctcatccat cgctgacctc
     4501 atatccgacc gatggaaact acctgcgaag aagccccggt attccagacc gcggcgcaag
     4561 gcacagagcg gcacctctcc ccaccgtcat ggccggcgaa ggccggctac ccacgtcttt
     4621 tcaacaaggg cttcggcgac atcgcccacc gtatcatttc ctgaatattt tccttgcctc
     4681 cctaaccaaa ttccgttagt tataccatca gcatggaacc agacacacca tccgcctttc
     4741 ctccagatcc accacaggag cagtgcgcct ccaacgtcga gtacctcctc cacgccgtca
     4801 gcatcctcct cgtctacggc cgccacctcg tcgacaccat ccgccagcgc gccaccactc
     4861 cccacttcaa cgccatcgct gcctgcttcg gcaccgccaa ccttgccacc atcctcgctc
     4921 atctgaaccg tggggtccgg cgcgc
//
LOCUS       DATGEM010000576         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_740924, whole genome shotgun sequence.
ACCESSION   DATGEM010000576 DATGEM010000000
VERSION     DATGEM010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740924"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VKQ27_17625"
     CDS             complement(<1..530)
                     /locus_tag="VKQ27_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLY90806.1"
                     /translation="MESEQAYLPAALEALAAFGVEPAEVRFVHLSENVTFRATDARDG
                     SQLVLRLHRPWYHDIAALQSEHLWTRALVQAGVAAPEPLLTSTGESFAQIDIVATGER
                     RWAGLARWVEGELLGDVVARETDSAVNARHFAQLGVIMAAMHNQATGWTPPDGFERHA
                     LDADGLMGPEPFWGPF"
     gene            complement(595..1878)
                     /locus_tag="VKQ27_17630"
     CDS             complement(595..1878)
                     /locus_tag="VKQ27_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLY90807.1"
                     /translation="MTDTTSLLARRNRALGAGAPLFYEHPLHIVRGEGCYMYDPDGRR
                     YVDMYNNVPCVGHANPRVVQAMATQQATLNVHSRYLHEAVVEFCERLAALHRPEIESV
                     ILSCSGTEANEVALRMARFATGKRGILCTDATYHGNSELVSSLNRLGRSPSPNPSVRA
                     FPFPEKLRPIGSGLTEEALCEAYLAKLDAAIADLQSSGVGVAAMIVCSILANEGLPDI
                     PTGFMSRAAAKVREAGGLVIADEVQAGYGRTGHWWGYEVTGFTPDIVVTGKPMGNGLP
                     LAATAASQVLVETFRARTRYFNTFASSPLQAAVGMAVLDEIEQRDLRGNVAIVGAALR
                     AALSQRKDASKAIGDVRGQGLFIGIEMVKPADNAPDTELAKSVINRLKDKGFLTSNAG
                     AFANVVKIRPPLPFTQADASAFLVAWDETIAELAA"
     gene            complement(3053..3985)
                     /locus_tag="VKQ27_17635"
     CDS             complement(3053..3985)
                     /locus_tag="VKQ27_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLY90808.1"
                     /translation="MKVLFHYAAGPDLSARLGAIPDLTIIACPESDHALLARLLPETD
                     VLWHVLERCTAEMIAAAPRLRLIQKIGVGVNTIDLDAAKARGIPVCNLPGTNARAVAE
                     LTLALMLAVLRRIPRFDAAMRRGEWSDTALQDGIGELGGRTVGLVGYGAIPQLLAPVL
                     VALGCRVIYTARAPRAEAAGEWRSLDALVAEADIVSLHLPLVAETEGLIGARALARMK
                     PGGILINTARGGLVDQRALTEALESGRLSGAGLDVFEHEPHDASEVLSRLPNVVLTPH
                     VAWLTTGTFDRSFVLAAENCRKLAAGEGLLHRVV"
     gene            complement(3982..>4846)
                     /gene="eno"
                     /locus_tag="VKQ27_17640"
     CDS             complement(3982..>4846)
                     /gene="eno"
                     /locus_tag="VKQ27_17640"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLY90809.1"
                     /translation="MLPLPQIQIFGGGAHAGRRVDIQDFMVTCPAAETFAEALDWTAE
                     VYRAAGLLMKEAGTLQGVADEGGWWPAFSTNEQALETLVRAIERAGYEPGTEVAIALD
                     IAASEFGRGGKYKLALEDRELDSEGMVKLLSGWVRRYPIVSIEDPLAEDDAEGFAAFT
                     REVGQRVQVVGDDFLVTQASRVREAAIRGAANAVLLKPNQRGTLTETFEAWQAAKEAG
                     FAGIVSARSGETEDVSIVHLAVGWGVGQLKVGSFARSERMAKWNEALRIEEVLSVRAS
                     FAGSGALRGRA"
BASE COUNT          890 a         1682 c         1444 g          830 t
ORIGIN      
        1 cagaatggcc cccagaacgg ctccggcccc atcagaccat cagcgtccag cgcgtgccgt
       61 tcgaacccgt cgggcggcgt ccagcctgtc gcctggttat gcatcgccgc catgatgacg
      121 cccagctgcg cgaagtggcg cgcattcacc gcgctatcgg tttcacgtgc aaccacatcg
      181 ccgagcaatt cgccttccac ccagcgcgcg agcccagccc agcgccgctc cccggtggcg
      241 acaatatcga tctgggcgaa gctctctccg gtcgacgtca gcaacggctc gggggccgcg
      301 acgccagcct gcacgagggc gcgcgtccac aggtgctcag actgcagggc ggcgatgtcg
      361 tgataccagg ggcggtgaag ccgcaacacc agctgtgagc catcgcgtgc atcggtcgcg
      421 cggaaagtta cattctcgct caaatgcacg aagcgcacct cggcgggctc aacgccaaac
      481 gccgcaagtg cctccaacgc agctggcaga tacgcctgct cggactccat cgtcacgtat
      541 ggttccgggc gccaacccgg ccatccatcg tcacgggcgg gcttgaccca cccatcatgc
      601 tgcgagttcg gcgatcgtct cgtcccaggc gaccagaaag gccgacgcgt cagcctgcgt
      661 gaacggcagc ggcggccgga tcttcacgac attcgcaaaa gcgccggcgt tgctggtcag
      721 gaagcccttg tccttcagcc ggttgatcac actcttggca agttccgtgt cgggcgcgtt
      781 gtccgccggc ttcaccatct cgatgccaat gaacaggccc tggccacgca catcgccgat
      841 cgccttgctg gcgtccttgc gctgactcaa cgcagccctc aaggccgcac cgacgatcgc
      901 cacgttgccc cgcaggtcgc gctgttcgat ttcatccagc accgccatgc cgactgccgc
      961 ctgcaacggg gaggacgcaa acgtgttgaa atagcgcgtc ctggcacgga aggtctcgac
     1021 cagcacctgg cttgcggcgg tggcggcgag gggcaatccg ttgcccatag gcttgccggt
     1081 gacgacgatg tctggcgtga acccggttac ctcgtagccc caccaatgtc cggtgcggcc
     1141 gtagcccgcc tgcacttcgt cggcgatcac aagaccgcct gcttcacgca ccttcgctgc
     1201 ggcgcgcgac atgaagccgg tgggaatgtc aggcaggccc tcatttgcca ggatcgagca
     1261 gacgatcatc gccgcaacgc ctacgcccga cgattgcaga tcggcgatcg cggcgtccaa
     1321 tttcgccaga tacgcctcgc acagcgcctc ttcggtcagg ccggatccga tcggacgcaa
     1381 cttctccggg aatgggaagg cgcgtaccga cggattcggc gatggcgagc ggccgagccg
     1441 gttgagcgaa ctgaccaact cgctgttgcc gtgataggtc gcatcggtgc agaggatgcc
     1501 gcgcttcccg gtggcgaagc gggccatgcg cagcgccact tcgttagcct cggtgccgct
     1561 gcaactcagg atgacagact cgatctctgg cctgtgcaga gcggcgagac gttcacagaa
     1621 ttcgacgaca gcttcatgca ggtagcggct atgcacattc agcgtcgcct gctgcgttgc
     1681 catcgcctga accacccgcg ggttggcgtg accgacacaa gggacattgt tgtacatgtc
     1741 gacgtaacgg cggccgtcgg gatcatacat gtagcagccc tcgccccgga cgatgtgcag
     1801 tgggtgctcg tagaacagcg gggcaccggc gcccagcgcg cggttgcgac gggcgagcag
     1861 gctggtcgtg tcggtcatgt gccctccgag ggccgggatg cgttggcgcg agcgggagtt
     1921 tacgatgcga tcccggctca cgccatggcc tgtcccaatg cacggacgat tcgctggccc
     1981 gtcaaagcga cagattgtgc agcgatatcg gcgtctaggc agcgggcgcc gccaccgcca
     2041 tcaacgccta cacgatcgga tatacttttc acgatagcag tttctcgtcc gacgctgcgc
     2101 aatggttagg gccggagccg ggttcaaggg tacagtaacg gcgtggccgc gtgcacgccg
     2161 gcagctcgac acatggagcg atcttgacgg taccagatct gagccgacgc tatcgcggtc
     2221 gattgatctc cagccgcctt gtcgtagctt tccgcaattc ccaggaggag aaatcgcacg
     2281 ggatcgtggc ccatgctatc ggcgagctgc ctcgcctgga tcgcacggct tttccaccgt
     2341 gctgcagttg ttatcaatgt gggctgatcc cgcattttga gaaaactcgc ttaatatgga
     2401 tttgattgtc ggggggcgat gtaagatgaa gtggttgctt gattgcaatc aaattctgaa
     2461 gttcaagcca aaaatctttg gcgacgcgag ggtcaggcgg cagctacagc acgcagcgct
     2521 cgctcaataa ttgcaatgac cgggatggtt gccggcggca gtcgccgcaa atttgcatcg
     2581 gcggcatggt gctgcccttt tctggacgat cggggcccgt gagcggcagt gacggacagg
     2641 ctgcacgaac gacctgcgcc aagaaacggt gaagtacaac tagccttcgt gtcgccggtg
     2701 gcctcacgac ccggtcccag cggccacccc gacagcgcag gcgtgcccga acaccccatc
     2761 cgcccaaaaa gacacgcccc gccgatcccg gcggggcgct gcgtttccat cctaaaccta
     2821 gctcgtggca gcttgcttac tcgcgagtag cgtccgtgtc caccggaaat gcggatggtg
     2881 cgttgcaaaa actcacttgc acacaaacga acaagggcct attttccgca tcgcagcaat
     2941 gcggcccctg gccgcgctgt ctgctttctt ggacgtttcc tccctaaact tggcggcccg
     3001 cagcggccgc catttcttta tctgcgctgg ggcggcggcc ggtcccggtc gctcagacca
     3061 cccggtggag caagccttcg cccgcggcaa gtttccggca attctctgca gccagcacaa
     3121 agctgcggtc gaacgtgccg gtggtcagcc acgcgacatg cggcgtcagc acaacatttg
     3181 gtagtcggga cagcacctcc gaggcgtcgt gcggctcgtg ttcgaacaca tcgaggccag
     3241 cgccggacaa acgtccgctc tccagcgcct cggtcagcgc acgctgatcc accagccctc
     3301 cccgcgccgt gttgatcagg atgccgccgg gcttcatccg cgccagtgca cgtgcgccga
     3361 tcagaccctc ggtttccgcc accagaggaa gatgcaggct gacaatatcc gcctcggcaa
     3421 ccagcgcatc cagggagcgc cattcgccgg ctgcctccgc ccgaggcgcg cgcgcggtgt
     3481 agatcacgcg gcagcccaac gcgaccagca ccggcgccag caattgcgga atggcgccgt
     3541 aaccgacgag acccacagta cggccgccaa gttcgccaat cccgtcttgc aacgccgtgt
     3601 cggaccattc gccgcgccgc atcgccgcgt cgaaccgcgg gatgcgccgc agtaccgcga
     3661 gcatcaacgc cagcgtcagt tcagcaaccg cgcgtgcgtt ggtgcccggc aagttgcaca
     3721 ccggaatgcc ccgcgccttc gccgcgtcca gatcaattgt gttgacgccc acgccgatct
     3781 tctggatcag ccgcagccga ggcgcggcag cgatcatctc ggccgtgcag cgctcgagta
     3841 cgtgccacaa tacgtccgtc tcaggcagca gccgcgccag caatgcgtgg tcgctctccg
     3901 ggcaggcgat gatcgtgagg tcaggaatcg cacccagccg cgcactcagg tccggacccg
     3961 cggcatagtg gaacagaacc ttcaagcgcg cccccgcagc gcgccgctgc cagcaaaact
     4021 ggcacgcacg ctcagcactt cctcgatgcg cagcgcctcg ttccatttcg ccatccgctc
     4081 gctccgggcg aagctgccaa ccttgagttg cccgaccccc cagcccacgg caagatgcac
     4141 gatcgaaaca tcctcggtct caccggaacg agccgacacg atccccgcga acccggcctc
     4201 cttcgctgcc tgccacgcct caaacgtttc ggtcagcgtg ccccgctgat tcggcttcag
     4261 cagcaccgca ttggccgcgc cccggatcgc cgcttcccgc acacgggatg cctgcgtcac
     4321 aaggaaatcg tcgccaacca cctgaacccg ctggcccacc tcgcgggtga acgctgcaaa
     4381 gccttcagca tcgtcctcgg ccagcggatc ctcgatcgag acgatcggat accgccgcac
     4441 ccagccggac agcagcttca ccattccctc gctgtccaac tccctgtcct ccaatgcgag
     4501 cttgtattta ccgccacggc cgaactccga ggcggcaatg tcaagcgcga tggccacctc
     4561 ggtccccggc tcgtagcccg cccgctcgat ggcacgcacc agcgtctcca gggcctgttc
     4621 attggtggag aacgccggcc accagccgcc ctcgtcggcg acgccctgca gcgttcccgc
     4681 ctctttcatc agcagaccgg cagcgcgata cacctcggcg gtccaatcca gcgcctctgc
     4741 gaacgtctcc gcagccggac aggtgaccat gaaatcctga atatcgacgc gccgcccggc
     4801 atgcgcgcca ccgccaaaga tctggatctg cggcagcggc agcatg
//
LOCUS       DATGEM010000577         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_496021, whole genome shotgun sequence.
ACCESSION   DATGEM010000577 DATGEM010000000
VERSION     DATGEM010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496021"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VKQ27_17645"
     CDS             complement(<1..952)
                     /locus_tag="VKQ27_17645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HLY90810.1"
                     /translation="MAPAISDAQYQDQVLPHVSRTFALTIPQLPAALRIPVTNAYLLC
                     RIADTIEDEPALSPAETLAFMQRFMGALAGGSDPHTLGLELAKRLSDRTLASERDLVS
                     NLERVINVTAALNAPQRTAILRCVELMCYGMPRFQSTASLRGLARLSDLDDYCYYVAG
                     VVGDMLTELFCDYSPAIAKNRAGLASLGVSFAQGLQMTNILKDVWEDRSRGACWLPQD
                     VFSRQGVDLSRLTPVPFDPRFGDGMRELVAVAHAHLRNALEYTLLIPGGEGGIRRFCL
                     WAIGMAVLTLQKINQKPDFTAGVQVKISRRAVALTRISTDF"
     gene            1087..>2847
                     /gene="dxs"
                     /locus_tag="VKQ27_17650"
     CDS             1087..>2847
                     /gene="dxs"
                     /locus_tag="VKQ27_17650"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HLY90811.1"
                     /translation="MRRCGDAATCGAAHYPSGPRRYKLRQMVEPPTKYPLLETIGIPA
                     DLRRLPQPKLAQLAAELREFLIHSVSTRGGHFAAGLGTVELTVALHYVFNTPYDRLVW
                     DVGHQAYPHKVLTGRRELLHTIKQDKGLAPFPTRSESEFDTFGVGHSSTSISAALGMA
                     IAAAKRGEDRRAVAIIGDGALTAGMAFEALNHAGTAPADLLIILNDNDMSISENVGAL
                     SNYLARALSGRMYAHLREGGKKVLRQMPTVWELARRSEEHLKGMVLPGTLFEEMGFNY
                     IGPVDGHDVKALVATLNNVKQLKGPQFLHVVTRKGKGYAPAEADPIKWHGPGPFDPAS
                     GKIFKEASSGPTYSQIFGQWLCDMAERDPNVIGITPAMREGSGLVEFSKRFPDRYFDV
                     AIAEQHAVTFAAGLACEGFKPVVAIYSTFLQRAYDQLIHDVAVQNLPVVFALDRAGLV
                     GSDGATHQGSYDVSFLRCIPNMVVMTPSDENECRQMLFTGTTLSSPSVVRYPRGTGCG
                     VPVVAEMAALPVGRAQMRREGRSGLAILVFGTLLDSARKIAERLDATLVNMRFVKPLD
                     AALVESVAERHQAIITVEENA"
BASE COUNT          533 a          906 c          915 g          493 t
ORIGIN      
        1 cgaaatccgt cgagattcga gtcaatgcca ccgcccttcg agaaattttc acctgcaccc
       61 ctgccgtaaa gtccggtttt tgattaattt tttgcaatgt cagcaccgcc attccgattg
      121 cccacaggca aaagcggcgg attccgccct cgccgcccgg aatcagcagc gtgtattcga
      181 gagcgttgcg cagatgcgca tgggcgaccg cgaccagttc gcgcatgccg tcgccgaagc
      241 gcgggtcgaa cggcaccggc gtaagccgcg acaaatccac gccctgacgc gagaacacgt
      301 cctgcggcag ccagcaggcg ccacggctgc gatcttccca cacatctttg aggatattcg
      361 tcatttgcag cccttgagca aacgacacgc cgaggctcgc gagcccggcg cggtttttgg
      421 cgatggcggg cgaataatcg cagaacagct ccgtgagcat gtcgcccacg acaccggcca
      481 cgtagtaaca ataatcgtcc agatcgctga ggcgagccag cccgcgcagg ctcgcggtcg
      541 actgaaatcg cggcattcca tagcacatga gctcgacgca gcgaagtatc gcggtacgct
      601 gcggggcgtt cagtgcggcg gtgacgttga tgacccgctc gaggttgctc acgaggtcgc
      661 gctcggaggc aagcgtgcga tcggagaggc gtttcgcgag ttccagcccg agcgtgtgcg
      721 gatcgctgcc gcctgcgagc gctcccatga aacgctgcat gaatgccaat gtttcggcgg
      781 gcgagagcgc cggctcgtcc tcgatcgtgt cggcgatgcg gcacagcagg taggcattgg
      841 tcaccgggat tcgcagcgct gccggtaatt gcgggatcgt cagcgcgaag gtgcgcgaga
      901 cgtgcggcaa tacctggtct tgatactgtg catcggatat tgcaggggcc atggaggaca
      961 gtatctcgga aattcgcggc gatagcacca cccggccggg tcggttgtgg cgggaaacac
     1021 ccggatgcgc catcgcgttg gcagaccgga aagctgctgc aggcgttacg gaacgccctt
     1081 cgcggcatgc ggcgctgtgg cgatgccgcc acatgcggcg cggcccatta cccgtccggc
     1141 ccgcgacggt ataaactgcg ccagatggta gaaccaccaa cgaagtaccc cctgctcgag
     1201 accatcggca tccccgcgga tttgcgccgg ttgccgcagc cgaaactggc tcagctggcc
     1261 gcggaactgc gcgaattcct gatccacagc gtcagtaccc ggggcggcca tttcgccgca
     1321 gggttgggga cggtggaact gaccgtcgct ctgcactatg tcttcaatac gccctatgac
     1381 cggctggtgt gggatgtggg ccaccaggcc tatccgcaca aggtgctgac cggacggcgc
     1441 gaactgctgc acaccatcaa gcaggataag ggcctggcgc cctttccaac gcgcagcgaa
     1501 agcgagttcg acacctttgg cgtcggccat tccagcacct ccatcagcgc ggcactcggc
     1561 atggccatcg cggccgccaa acggggcgag gatcggcgcg cggtcgccat catcggcgac
     1621 ggtgccctga ccgccggcat ggccttcgag gccctgaatc atgcaggtac ggcaccggcc
     1681 gatctgctca tcattctgaa cgacaacgac atgtcgatct cggagaacgt cggcgctctc
     1741 tccaactacc tggcccgcgc cctgtcgggg cgcatgtatg cgcatctgcg cgagggcggc
     1801 aagaaggtat tgcggcagat gccgacggtg tgggagctcg cgcgccgttc cgaagagcac
     1861 ctgaagggaa tggtgctccc cggcaccctg ttcgaggaaa tgggcttcaa ctacataggg
     1921 ccggtcgacg gacacgacgt caaggcgctg gtcgcgacgc tcaacaatgt caagcagctg
     1981 aagggcccgc agttcctgca cgtcgtcacg cgcaagggca agggctatgc gccggcggaa
     2041 gcggatccga tcaaatggca tggaccgggc cccttcgatc cggccagcgg caagatcttc
     2101 aaggaagcga gttcgggccc gacgtattcc cagatcttcg gacagtggct gtgcgacatg
     2161 gcggagcgcg acccaaacgt catcggcatc acacccgcca tgcgcgaagg ttcgggcttg
     2221 gtcgaattct ccaagcgctt ccccgaccga tatttcgacg tggcgattgc cgagcagcat
     2281 gccgtgacct ttgccgcggg gctggcgtgc gaaggcttca aacccgtcgt cgccatctac
     2341 tcgacgttcc tgcaacgcgc ctatgaccag ctcatccacg acgttgccgt gcagaacctg
     2401 ccggtggtct tcgcgctgga ccgcgcgggc ctggtcggca gcgatggcgc gacgcatcaa
     2461 ggcagctacg atgtttcgtt cctgcgctgc attccgaata tggtggtcat gacgccgtcg
     2521 gacgagaacg agtgccgcca gatgctgttc actggcacga ccctgtcctc gccgtcggtg
     2581 gtgcgctacc cgcgggggac cgggtgcggc gtgccggtcg tcgcggagat ggccgcgctt
     2641 cccgtaggac gggcccaaat gcggcgcgaa ggccgcagcg gactcgcgat cctggtcttc
     2701 ggcacgctgc ttgactcggc gcgcaagata gccgagcggc tcgacgccac gctggtcaac
     2761 atgcgcttcg tcaaaccgct ggatgcggct ctcgtcgaga gcgtggccga gcggcatcag
     2821 gccatcatca ccgtcgagga aaacgcc
//
LOCUS       DATGEM010000578         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_413262, whole genome shotgun sequence.
ACCESSION   DATGEM010000578 DATGEM010000000
VERSION     DATGEM010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413262"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VKQ27_17655"
     CDS             <1..385
                     /locus_tag="VKQ27_17655"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLY90812.1"
                     /translation="DEIYAASSMRGPDSSSPVRCKLVGWWQGSRSDALAKELYGDSNG
                     SHATASEVSLAQHYHPEAIKHAQMSPKVAPKSKGFFDSADYRRRFPDGRIGSDPSLSS
                     PEHGARIYEAAVVGMVEVYQAFMAD"
     gene            385..2142
                     /locus_tag="VKQ27_17660"
     CDS             385..2142
                     /locus_tag="VKQ27_17660"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY90813.1"
                     /translation="MERLDPIKLEIIRGAIRAAQAEMDALLERTAISAFIREKKDFYT
                     ALFDADGIMVVGSMVPIFGDITGPVFARFAPDKMREGDLYWYSDCYASRGAVSHSNDQ
                     VFLAPVFHNGKRAAFVMGWAHFSDIGGLRPGSISSDATDIFQEGIIIPPTKLIDAGVV
                     NEAALEIFHRNSRYPEQSRGDTRALMASVDLGVRRVREILERFGPDVMADAFAQLFTR
                     TRELVRAKLRETFAVGTHRFSDAIDSDGHGNGPFFIRLALTRTPDDRFILDATATDDQ
                     APGPVNFLMNPDVPGMALGLFFLGGDPAQVCNAGGPRALDEVKLREGSLLWPRFPAPL
                     GLRGMTMMRFLAVLNGLVNVAGGSAPAANSAYVVIMLRGTAGGKQFLMSDGVGVGYGA
                     RATADGIDAVYFVAQENYPVEFLELGYPVRLLRYGINRDSGGPGRFRGGAGIVRQYEV
                     LAEHAILSMRIDSVLNPPWGVAGGMQGGTGRAIVNPGTPHERELAPLSDGTVLRRGDI
                     LLLETGGGGGHGHPFDRPAERVLQDVRDGFVSSEAARRDYGVAIVDNAVDDMTTQTLR
                     SPRPLVKAFHRKGYVDAIG"
     gene            2129..4144
                     /locus_tag="VKQ27_17665"
     CDS             2129..4144
                     /locus_tag="VKQ27_17665"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY90814.1"
                     /translation="MPSADLTVAVDIGGTFTDITLADRTTGQLWRAKTPSTPRDPSEA
                     FLTGVMLALTQADARPQDVTRALHGTTVATNLILEGKTARTALVTTAGFRHVLEIGRQ
                     DIPRAANLFTWVKPQRPVPPAHVLEVQERVGPGGVVLLPLDEASVTEAAATLRQLDVQ
                     AVAICLLHSFAHPAHERRVAGLLRAALPDVAVTASVDVLPVVREYERSLATIMNAGVM
                     PAVTTYVQRLQRRLTDADIAAPLLLMQSNGGVAGAATIGRAPALTVLSGPAAGVVGAR
                     DVAAAAGIGDIVTVDIGGTSADICLLRSGQIALTQRGRIGEWPLPLPMVDMVTIGAGG
                     GSIARLSSGALTVGPASAGAVPGPACYGMGGEEPTVTDAHLVLGHLPASLLDGRMTLD
                     PALAEQAIRTRVAEPLGLSLHQAARGILAIADSNMVGAIRVVSVERGHDPRDFALVAF
                     GGAGPLHGCALAALLGITRVLIPPAPGVLCAEGLLAASLKAEFSRTLPQPDEWDAADA
                     VYTALEAEAQSWLAEERVAPADAKLTRMALLRYEGQGSEIAIAWPGTADGARAAFAEA
                     HRALNGFVMESKVELVTLRVEAEGMIPPAPRTALPPGKGAQPIGQQMVHDTAGPVEAQ
                     IYQRAGLGVGDRIAGPAIITQLDATTLVAPAWYLEVLASGALLLRPQ"
     gene            4275..4754
                     /locus_tag="VKQ27_17670"
     CDS             4275..4754
                     /locus_tag="VKQ27_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90815.1"
                     /translation="MLTHAHAAVAPRSAPPSLQLQPPEPWADLLDRLGSIEDETSIIV
                     FGRDGPDLMCALLRAGAPNVTHLCSHERLEADSASLVIIPHLPSLDWLQGAFPALRRA
                     LSTNGRIVLCADPLSSMQTDIRRSLTLNGFTAIRAERASGRLVLSAELPAFGLRRCA"
     gene            4769..5701
                     /gene="dapA"
                     /locus_tag="VKQ27_17675"
     CDS             4769..5701
                     /gene="dapA"
                     /locus_tag="VKQ27_17675"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLY90816.1"
                     /translation="MHNAVAGLGGSLTALVTPFRESCVDWKALCHLTERQIERGTSAL
                     IVCGSTGEAASLILPEYSRAVRTVAEMSAGRAPVIVGCTALATAAAVAMGAEAAAAGA
                     DALLCAVPPYVKPTQCGIIAHIRAIAHATDLPIVLYDVPSRSGAAISDDTIARLYDAE
                     LIVGLKDATADLSRPARLRARCGSAFLQWSGDDATAPAHRAMGGVGCISVTANVAPAL
                     CALLHRCWDAGDMARFATLRDVLDPLHAALFNESNPIPVKSALASLGLCTDEMRLPLT
                     RANLTTQEWLLTVMPTVMLAEEHAAHQPVIALAS"
BASE COUNT          927 a         1930 c         1938 g         1079 t
ORIGIN      
        1 cgacgagatt tacgcggcgt cctcgatgcg ggggccggat tcgtcgtcgc cggtgagatg
       61 caagctggtt ggttggtggc aggggtcgcg ttccgacgcg ctggccaagg aactctatgg
      121 cgactccaat ggcagccacg ctacggcgtc cgaggtatcg ctggcgcagc attaccaccc
      181 cgaggcgatc aagcacgcgc agatgtcgcc gaaggtggcg ccgaagagca agggcttctt
      241 cgactccgcg gactatcggc ggcggtttcc ggacgggcgc atcgggtccg atccgtcgct
      301 gtcttccccc gagcatgggg cgaggatcta tgaagccgcg gtggtcggta tggtcgaagt
      361 ctatcaggcg ttcatggccg actgatggaa cgcctcgacc ccatcaagtt ggaaatcatc
      421 cgcggcgcca tccgcgcggc gcaggcggaa atggacgcgc tgctggagcg caccgcgatc
      481 agcgccttca tccgcgagaa gaaggatttt tacaccgccc tgttcgacgc cgacggcatc
      541 atggttgtgg gttcgatggt gccaatcttc ggcgacatca ccggaccggt cttcgcgcgg
      601 ttcgcgccgg ataagatgcg cgaaggcgac ctctactggt acagcgattg ctacgcgtcg
      661 cgcggagcag tctcgcactc caacgatcag gtgttcctgg cgcccgtatt ccacaacggc
      721 aagcgcgcgg cgttcgtcat gggttgggcg catttctccg atatcggcgg attgcgcccc
      781 ggctcgatca gctcggatgc gacggatatc ttccaggaag gcatcatcat cccgcccacc
      841 aagctgatcg atgccggcgt tgtgaacgag gcggcgctgg agatcttcca tcgcaactcg
      901 cgttatccgg agcagagccg aggcgatacg cgtgcgctca tggcatccgt cgatcttggc
      961 gtccgccgcg tgcgcgagat cctcgagcgc ttcggccccg atgtgatggc cgacgccttc
     1021 gcccagctct tcacccgcac gcgcgaactg gtgcgcgcga agctgcgtga gactttcgcc
     1081 gtcggcacgc atcgcttcag cgatgcgatc gattccgatg gccacggcaa cggccctttc
     1141 ttcatccggc tggctctgac gcggacgccg gatgaccggt tcatcctcga tgcaaccgcg
     1201 acagacgatc aggcacctgg tccggtgaat ttcctgatga accccgatgt gccagggatg
     1261 gcgctggggc tgttcttcct cggcggcgat ccagcgcagg tgtgcaatgc cggtggccca
     1321 cgcgcgctcg atgaggtcaa gctgcgcgag ggctcgctgc tgtggccgcg tttccccgcg
     1381 ccgctcggat tgcgcgggat gacgatgatg cggttcctcg cggtgctgaa tgggctggtc
     1441 aatgtcgcgg gcggcagcgc gcccgcggcg aattcggcct acgtggtgat catgctgcgg
     1501 ggcaccgccg gcgggaagca gttcctcatg tcggacggcg ttggcgtcgg ctacggcgcc
     1561 cgcgcgactg ccgacgggat cgacgccgtg tatttcgtcg cgcaggagaa ctacccggtt
     1621 gaattcctgg agctcggcta tccggtgcgg ctgctgcgct atggcatcaa ccgcgacagc
     1681 ggcggcccag gcaggttccg tggcggcgcc ggcatcgtgc ggcaatacga ggtgctggcc
     1741 gaacacgcca tattgtcgat gcgtatagac agcgtgctga atccgccatg gggcgttgcc
     1801 ggcggcatgc aaggcggcac cggccgcgcc atcgtcaatc caggcacgcc gcacgagcgt
     1861 gagcttgcgc cgctgtcaga cggcaccgtg ctgcgccgtg gcgacatcct gctgctggaa
     1921 accggcggtg gcggagggca tgggcatccg ttcgatcgcc ctgccgaacg cgtgttgcag
     1981 gatgtgcggg atggctttgt atcgtcagaa gcggcgcgcc gggattatgg cgttgcgatc
     2041 gtcgataacg cggtcgatga catgacgacg cagacattgc gctcgcccag gccattggtg
     2101 aaggcattcc accgcaaggg ttatgtcgat gccatcggct gacctcaccg tcgcggtcga
     2161 tatcggcggc acgttcaccg acataactct ggccgatcgc accaccggac agctttggcg
     2221 tgccaagacg cccagcacgc cgcgtgatcc gtccgaggcg ttcctcaccg gcgtaatgct
     2281 ggccctgacg caggcggatg cgcggccgca ggacgtgacg cgcgctctgc acggcacgac
     2341 ggtggccacc aacctgatcc tggaaggcaa gaccgcacgc accgcattgg ttaccaccgc
     2401 tggctttcgt cacgtgctgg agatcgggcg acaggacatt ccgcgcgccg ccaacctgtt
     2461 cacctgggtg aagccacagc ggcccgtccc gccggcgcat gtgctggaag tgcaggaacg
     2521 tgtcggtcct ggcggtgtgg tgctgctgcc gctcgatgaa gccagcgtga ccgaagccgc
     2581 cgcgaccttg cggcagctcg atgtgcaggc cgtggcaatc tgcctgctgc attccttcgc
     2641 tcatcccgcg catgaacgcc gcgtggccgg gctgttgcgt gcggctctgc ctgatgtggc
     2701 agtcacagca tcagtcgatg tgttgcccgt ggtgcgcgag tacgagcgta gcctggcgac
     2761 catcatgaac gccggtgtga tgccggcggt caccacatac gtgcagcgac tgcaacggcg
     2821 gctgaccgac gcggatatcg ctgcgccgtt gctgctgatg caatcgaatg gcggcgtcgc
     2881 aggcgcggca acgataggcc gggcacctgc gctgacggta ctgtccggcc ctgccgccgg
     2941 cgtcgttggc gcgcgtgatg tggcggctgc cgccggtatc ggtgacatcg tgacggtcga
     3001 tataggtggc accagcgcgg atatctgcct cctgcgcagc ggacagatcg cgctgacgca
     3061 gcgcggccgt atcggcgagt ggccgttgcc tctgccgatg gtcgacatgg tgacgatcgg
     3121 cgccggtgga ggatcgatcg cgcgcttgtc gagtggtgcg ctgaccgtcg gtcccgccag
     3181 tgccggcgcg gtgccgggtc ccgcgtgcta cggcatgggc ggtgaggaac ccacagtaac
     3241 cgacgcacac ctggtgctcg gtcatttgcc cgcatcgctg ctcgacggcc gcatgacgtt
     3301 agatcctgcc ctcgccgaac aggccatccg cacgcgtgtc gccgaaccgc tcggactttc
     3361 gctccaccag gccgcgcgcg gcatccttgc cattgcagac tccaacatgg tcggtgcgat
     3421 ccgcgtggtt tccgtcgagc gcgggcatga tccgcgtgat ttcgcgttgg tggcgttcgg
     3481 cggcgccggt ccgctgcacg gctgcgcgct tgccgcgctg ctgggcatca cgcgtgtgct
     3541 gatcccgccg gcgccgggtg tgctgtgcgc tgaaggtctg ctcgccgcgt cgctcaaggc
     3601 cgagttctcg cgcacgttgc cacagcccga tgaatgggac gccgccgatg ctgtctatac
     3661 agcactggaa gccgaagcac agtcgtggct ggcggaggaa cgtgtcgcgc cagccgatgc
     3721 caagctcacg cgcatggcat tgctgcgcta cgaagggcaa ggctcggaga ttgccatcgc
     3781 ctggcccggc acggccgacg gcgcgcgcgc ggcatttgcc gaggcgcatc gcgcgctgaa
     3841 tggcttcgtc atggaatcga aagtggaact cgtgacgctg cgggtggaag ccgaaggcat
     3901 gatcccccct gccccacgca cagcgctccc accagggaaa ggcgcgcagc cgatcggcca
     3961 gcagatggtg cacgatacgg cgggcccagt tgaggcacag atctaccagc gtgccggcct
     4021 cggcgtcggc gaccgcatcg ccgggcctgc catcatcact caactcgacg ccaccacgct
     4081 cgtggcaccc gcctggtatc tcgaggtcct ggccagcgga gcgctgctgc tgcgccccca
     4141 gtaacccaac aacaatccta tatgtgccaa ccacgacact gatcgcattt atatgcggtc
     4201 gcgtcgatct tcagcggcgt catgaatcgc gcaccattca ggttcgcaca ttcatccgtc
     4261 caggaggatc cttcatgttg acccatgccc atgcggcggt cgcgcctcgc agcgcgccgc
     4321 ccagcctgca attgcagccg ccagaaccat gggccgacct gctcgaccga cttggttcaa
     4381 tcgaggacga aaccagcatc atcgtcttcg gcagggacgg cccggatctg atgtgtgcgc
     4441 tgctgcgggc cggcgctccg aacgtcaccc acctctgctc gcatgaacgg ttggaagccg
     4501 acagtgccag ccttgtgatc atccctcacc taccttcgct cgactggctg cagggggcgt
     4561 ttccggcgct tcgccgcgcc ctcagcacca acggtcgcat cgtgctctgc gccgatccac
     4621 tgtcgtccat gcaaaccgat attcgccgca gcctgacgct gaatggcttc accgcgatcc
     4681 gggcggagcg cgcatccggc cggctggtac tgagcgccga gcttccggcg ttcggtctcc
     4741 gccgctgcgc ctgacaggag tcacgaccat gcataatgcc gttgccggtc ttggcggttc
     4801 gctgaccgcg cttgttacgc cgttccgcga gtcctgcgtt gactggaaag cgctgtgcca
     4861 tctcaccgag cgccagatcg agcgaggcac gtctgcgctc atcgtgtgcg gcagcaccgg
     4921 tgaggcggcc tcgctgattc tgcccgaata ttcgcgggct gtgcgtaccg tcgcggaaat
     4981 gtctgccgga cgcgcgccgg tcattgtcgg ctgcaccgcg ttggcaacag ccgccgcggt
     5041 cgccatgggc gcagaggctg cggccgccgg cgcggatgcc ctgctctgcg ccgttccgcc
     5101 ctacgtgaag ccgacacagt gcggcatcat cgcccatatc cgcgccatag cgcatgcgac
     5161 cgatctgcca atcgtgcttt acgatgtccc gtcgcgctcc ggtgccgcga tcagcgatga
     5221 caccatcgca cggctctacg acgccgagct gatcgttggt ctgaaggatg ccacggccga
     5281 tctgtcgcgt ccggcgcggc tgcgtgcgcg ctgcggcagc gctttcctcc aatggagcgg
     5341 cgatgacgca accgcaccgg cgcatcgggc catgggtggc gtcggctgta tctcggtcac
     5401 cgccaatgtg gcgccggcgc tgtgtgcgct gctgcaccgg tgttgggatg caggtgacat
     5461 ggcgcggttc gccacgctgc gcgatgtgct cgatccgctg cacgccgccc tgttcaacga
     5521 gagcaatccc attcccgtga aatcggcatt ggccagtctc ggcctgtgca ctgacgagat
     5581 gcggctgccc ctgacgcgtg ccaacctgac aacgcaggag tggttgctca ccgtcatgcc
     5641 aaccgtgatg ctggccgagg agcacgccgc gcatcagccg gtcatcgcgc tggcaagcta
     5701 gtcccctgtg tcattgcgtg gagcccggcg aaggccgggc gacgaagcaa tctcgaacac
     5761 ggtgcgcact accgcgcccc tgactaacga gtaaggatcg tgtttgatct gctgctcctg
     5821 gcctcgcttg cggtcgcctt tgctgctgcc gtcggctata tctacgcctg tgtt
//
LOCUS       DATGEM010000579         5351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_605634, whole genome shotgun sequence.
ACCESSION   DATGEM010000579 DATGEM010000000
VERSION     DATGEM010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605634"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VKQ27_17680"
     CDS             <1..371
                     /locus_tag="VKQ27_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HLY90817.1"
                     /translation="ALAIPLALMTGRTSSLLRTPEDTSPPSVVARAVALQREWMALPD
                     VSVKRLLSDAQLLSVHHAMLPPQRRPRIDPIDVVLLTARTKVEEAETLDCALEALSRP
                     ELAVALADEVSLTRLVGLAR"
     gene            complement(372..2675)
                     /locus_tag="VKQ27_17685"
     CDS             complement(372..2675)
                     /locus_tag="VKQ27_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90818.1"
                     /translation="MTDLRPYQNESNRFAQLSLKDLLDARDQYHIHLMRHPNVVATAV
                     GRYRIRNDEPWPSERGATRQVTGERTLTNSQVRPYSWPAVLVFVERWEDPDAFSGKDG
                     KYHPDEIVPRTLYLPDGRQIPVCVIVAPRREHTDIAAPPIRYPLNNIGGGYPVIADVQ
                     GQQHVATIACLVGDGHKVYALTNRHVTGDMGEVVYARLGGRQSRIGLSSGKQLTRMAF
                     AEMYPGWPGRDTFVNLDVGLIDIDDLDLWTAKIRDIGIMEPMADLSVDNISLSLIGCH
                     VRGYGAASGVMLGEIHALFYRYKSQGGFEYVSDLFIGPRSAANDHAKVPPSFATHPGD
                     SGTLWLLEPMEAAPKSATKYLPLAMQWGQNTLGSRAGAGTQTYVLATFLSRVCSLLTV
                     DPIRDWNLDQTDTWGAVGHFAIAARGEVALSGRFAKLTRLIENNRQIIGHDDNTILND
                     DFKGMGDAAFVPLADVPDFFWKHGKQGSSRGSEGPNHFADMDQPGPGGQTLLDLCKDP
                     AGIDPDRWNAFYDQVTDMLTGDPVSAEHRGLLPFRVWEIFDAMVGYAKQGDGQRFLCA
                     AGVLAHYVGDACQPLHISYLHDGDPEQPVTRTVHHRNGETEEVKDPLGKGVHSAYEDE
                     MVNAFRQDILDGLESTPKAQSSEYVSNGFEAAQRTVALMRATFEAIPPADIVQAFVSF
                     GKGKAGRAQSMWDKFGQGTIGVMRDGAHLLAVLWESAWSQGDGETHVHSTAALTQSAA
                     MEICADATFVPSQTIDRIGVILERPQN"
     gene            complement(2959..4833)
                     /locus_tag="VKQ27_17690"
     CDS             complement(2959..4833)
                     /locus_tag="VKQ27_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY90819.1"
                     /translation="MNYAEAYAAWRADPQAWWAATAEGITWTRRWDRVFDPDIPPYGR
                     WFPGAELNTCFNCIDRHVEAGRADQQALIWDSPMTGRIETYTYRQLQSRTARLAGALA
                     TLGVTKGDRVVIYLPMVPEALVAMLACARIGAIHSVVFGGFAAAELASRIADAKPKVI
                     VSASCGLEPGRIVKYKPLLDAAIALSPHKPDACLILQRDAARADLMPGRDHDLAEVEA
                     AAAPHDCVPVAATDPLYILYTSGTTGRPKGIVRDNGGHAVALWNSMAMLYGVEAGDVY
                     WAASDVGWVVGHSYIVYAPLLRGCTTVMYEGKPVGTPDAGAFWRVCAQHGVKVLFTAP
                     TAMRAIKQQDPEGKLLHAHDLSKLEALFLAGERCDPPTAVWAADLLGKPVVDHWWQTE
                     TGWGITSGFRQFGLFPFKPGSGGRASPGIDIHTLDDDGHEAAPGQTGNLCARLPLPPG
                     CAPTLWQNDEGYRAAYLSDFPGWYRTGDAGMIDKDGDVWVMGRTDDIINVAGHRLSTG
                     QMEEVLASHAAVAECAVVGARDELKGQTPIGLVVLKSGVNRPESEIVAELVALVRDRI
                     GPVAAFKDARVVPRLPKTRSGKILRASIRRIADGEQFTVPPTIEDPAALEEIAQALHH
                     "
     gene            4984..>5351
                     /locus_tag="VKQ27_17695"
     CDS             4984..>5351
                     /locus_tag="VKQ27_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90820.1"
                     /translation="MRGNVMWKAVASIMLVLGTMSAASAQTITDPNEIASHLHFWSQE
                     SGDPVYYGEVRNVSAISRQILGHYRCLALAFIKYENGVFYYAHKMWGKSPDGSCHYDD
                     VTSHKVDNHGICVKDTTAGFG"
BASE COUNT          897 a         1528 c         1960 g          966 t
ORIGIN      
        1 tggctctggc gattccgctg gcgctgatga ccggccggac gtcgtcgttg ctgcgcacgc
       61 cggaggatac gtcgccgccg tcagtggtgg cgcgggcggt ggcgttgcag cgagagtgga
      121 tggcattgcc ggatgtgtcg gtgaagcggc tgctttcgga tgcacagctg ctcagtgtgc
      181 atcacgcgat gctgccgccg cagcgtcggc cacggatcga tccgatcgat gtcgtgctgc
      241 tgaccgcccg gaccaaggtc gaggaggccg agacgctgga ctgcgcgctc gaagcgctca
      301 gccggccgga actggcggtg gcgctggccg atgaggtgtc attgacgaga ctggtcggcc
      361 tcgcgcgctg atcagttctg cggtcgttcg aggatgacgc cgatgcgatc gatggtctgc
      421 gacggcacga aagtggcatc ggcacagatc tccatcgccg cactctgcgt cagagccgcg
      481 gtggaatgaa catgcgtctc gccatcgccc tgtgaccagg cactctccca cagcaccgca
      541 aggaggtggg cgccgtcacg catcacgccg atggtgccct ggccgaactt gtcccacatg
      601 gactgggcac ggcccgcctt gcccttgccg aagctgacga aggcctggac gatgtcggcc
      661 ggcgggattg cctcgaacgt ggcgcgcatc agggcgacgg tgcgctgcgc ggcctcgaag
      721 ccattgctga catattcgct ggattgcgcc tttggcgttg actcgaggcc gtccaggata
      781 tcctggcgga aggcgttcac catctcgtcc tcgtaggcgc tgtggacgcc tttgcccaac
      841 gggtccttga cctcttccgt ctcgccgttg cggtgatgga cggtgcgggt caccggctgc
      901 tcgggatcgc cgtcatgcag gtaggagatg tgcagcggct ggcaggcgtc accgacataa
      961 tgggcaagca cgccggcggc gcagagaaag cgttggccat cgccctgctt tgcatagccc
     1021 accatggcat cgaagatctc ccagacgcgg aacggcagca gcccgcggtg ctctgcgctg
     1081 acgggatcac cggtgagcat gtcggtgacc tgatcgtaga acgcattcca gcggtcagga
     1141 tcgatgccgg ctggatcctt gcacaggtcg agcaaggtct ggccgccggg tccaggctgg
     1201 tccatgtcgg cgaagtggtt ggggccctcg ctgccgcgcg acgaaccttg cttgccatgt
     1261 ttccagaaga agtcaggcac gtcggccagt ggcacgaagg cggcatcgcc catgcccttg
     1321 aagtcgtcgt tcaggatggt gttgtcatcg tggccgatga tctggcggtt gttctcgatc
     1381 agcctggtga gcttggcgaa cctgccggac agcgcgacct caccgcgcgc cgcgatggcg
     1441 aagtggccga cggcgcccca ggtgtctgtc tgatcgaggt tccagtcgcg gatcggatcg
     1501 acagtgagca ggctgcatac acggctgagg aaggtcgcga gcacgtaggt ctgggtacct
     1561 gccccggcgc gcgaacccaa tgtgttctgc ccccactgca ttgccagcgg caggtatttc
     1621 gtcgcgcttt ttggtgctgc ctccatcggc tccagcagcc atagcgtgcc tgagtcgcct
     1681 ggatgcgtag cgaaagacgg cgggaccttg gcatggtcgt tcgcggcact gcgcgggccg
     1741 atgaaaaggt cggacacgta ttcgaagccg ccctgggact tgtagcgata gaacaaggca
     1801 tggatctcgc cgagcatcac accgctcgcc gcaccgtagc cgcgcacgtg gcagccgatg
     1861 agcgagagcg agatgttgtc caccgacagg tcggccatcg gctccatgat gccgatgtcg
     1921 cggatcttcg ccgtccacag gtcgagatca tcgatatcga tcaggccgac gtcgaggttg
     1981 acgaacgtgt cgcgacccgg ccagccagga tacatttcgg caaacgccat gcgggtgagc
     2041 tgtttgccgg agctgaggcc gatacggctc tgcctgccgc cgaggcgggc atagacgact
     2101 tcgcccatgt cgccggtgac gtggcggttg gtgagtgcgt agaccttgtg gccatcaccc
     2161 accaggcagg caatggttgc gacgtgctgc tggccctgga cgtcggcgat cacggggtag
     2221 ccgccgccga tattgttcag cggatagcgg atgggcgggg cagcgatgtc ggtgtgctcc
     2281 cggcgcggcg cgacgatgac gcagaccgga atctgcctgc cgtcgggcag gtagagtgtg
     2341 cggggcacga tctcatccgg gtggtacttg ccgtccttgc cgctgaatgc atccgggtcc
     2401 tcccagcgtt cgacgaacac caggacggcg ggccaggagt agggtcggac ctgcgagttg
     2461 gtcagcgtgc gctcgccggt gacttgccgg gtggcgccgc gttcagacgg ccagggctcg
     2521 tcgttgcgga tgcgataacg gccgaccgcg gtggcaacga cgttgggatg gcgcatgagg
     2581 tggatgtgat actggtctcg cgcatcgagc aggtctttca gcgacagctg cgcgaaacgg
     2641 ttggattcgt tctggtaggg gcggaggtcg gtcatcgcgg cttccctgtt tcccgccact
     2701 ctatggttgg cgggtagtgg ctcgtgtgaa ggcgatcaca gattgggggc gagcggcgtg
     2761 atctgttcat gacagggggc gggacgcatc acggggcgtt ccaagtgatc agctgcgatt
     2821 gtgctgcagg aacaatggcg gtccaggaag acgcgcaaag gaaagacgtg catgctcgca
     2881 cgggagcgcg agcatgacgt tgtggtacgt cgaggcgtgg gcatgatgtc gagagcgcac
     2941 ccagcgtcgt gacccgcttc agtgatgtag cgcctgggcg atttcttcca gcgcggccgg
     3001 atcctcgatg gtgggtggaa ctgtgaactg ctcgccgtcg gcaatgcggc ggatcgaggc
     3061 gcggaggatt ttgcctgagc gggtcttggg gaggcgaggg accacacggg cgtccttgaa
     3121 ggcggcgaca gggccgatgc gatcgcggac cagtgcgacg agttcggcca ctatttctga
     3181 ctcggggcgg ttgacgccgg atttcagcac gacgaggccg atgggggttt ggcctttcag
     3241 ctcatccctg gcaccgacga cggcgcactc ggcgacggcg gcgtgggagg cgaggacttc
     3301 ctccatctgg ccggtcgaga ggcggtggcc ggcgacgttg atgatgtcgt cggtacggcc
     3361 catgacccag acgtcgccat ccttatcgat catgccggcg tcgccggtgc ggtaccagcc
     3421 ggggaaatcg gagaggtacg cggcgcggta gccttcgtcg ttctgccaga gggtgggcgc
     3481 gcaaccggga ggcaggggca ggcgggcgca gaggttgccg gtctggccgg gggcggcctc
     3541 gtggccgtcg tcgtcgaggg tgtggatgtc gatgccggga ctggcgcggc cgccggaacc
     3601 tggcttgaag gggaacagtc cgaactggcg gaagccggag gtgatccccc agcctgtctc
     3661 tgtctgccac cagtggtcga ccaccggctt gccgagcagg tccgcggccc agacagcggt
     3721 gggcggatcg cagcgttcgc cggcgaggaa cagggcttca agtttcgaga ggtcgtgggc
     3781 gtggagcagc ttgccttcgg ggtcctgctg cttgatggcg cgcatcgcgg tgggggcggt
     3841 gaacagcacc ttcacgccgt gctgggcgca gacccgccag aaggcgccgg cgtcgggagt
     3901 gccgacgggc ttgccttcgt acatcacggt ggtgcagccg cgcagcagag gcgcgtagac
     3961 gatgtagctg tggccgacga cccagccgac gtcggaggcg gcccagtaaa cgtcgcccgc
     4021 ctcgacgccg tagagcatcg ccatggagtt ccagagggcg acggcgtggc cgccattgtc
     4081 gcggacgatg cccttggggc ggccggtcgt gcccgaggtg tagaggatgt agagcgggtc
     4141 ggtggcggcg accgggacgc agtcatgagg cgcggcggcg gcttcgactt cggccaggtc
     4201 gtggtctcgg ccgggcatca agtcggcacg ggcggcgtca cgttgcagga tcaggcaggc
     4261 gtccggcttg tggggtgaga gggcaatagc ggcatcgagc agcggcttgt atttcacgat
     4321 gcggccgggt tcgaggccgc aggacgcgga cacgatgacc ttcggtttgg cgtcggcgat
     4381 gcgggaagcg agttcggcag cggcgaagcc gccgaagacg acggagtgga tggcgccgat
     4441 gcgggcgcag gccagcatgg caacgagcgc ttcggggacc atgggcaggt agatcacgac
     4501 gcggtcgccc ttggtgaccc cgagagtggc gagtgcgccg gctagcctgg cggtacggga
     4561 ctgcagctgg cggtaggtgt aggtctcgat gcggccggtc atcgggctgt cccagatcag
     4621 cgcctgctga tcagcgcggc cggcttcgac atggcggtcg atgcagttga agcaggtgtt
     4681 gagttcggcg ccagggaacc agcggccgta gggtggaatg tcggggtcga agacgcggtc
     4741 ccagcggcgg gtccaggtga tgccctcggc cgtggcggcc caccaggcct gcgggtcggc
     4801 gcgccaggcg gcgtaggctt cagcgtagtt catggcggcc ctccctgcgg gtctgcgaca
     4861 ggagaatggc gcggaaaggg cagagagcgg aagggtttga tggctgaaag caacagtgag
     4921 tcggatgcgt agctgttgcg taatgtcaac ctctgtgaca ggctccgacg catgtagcta
     4981 caaatgcggg gcaacgtcat gtggaaagca gtggcttcga tcatgctcgt cttaggcacg
     5041 atgtccgcgg ccagtgccca gacgatcact gatccaaatg agatcgcctc ccatctgcat
     5101 ttttggtcgc aggagagcgg cgacccggtc tattatgggg aagtcagaaa cgtaagtgcg
     5161 ataagcaggc agatcctggg gcactatcgg tgcctggcgc ttgccttcat caagtacgaa
     5221 aacggagtgt tctattacgc gcacaagatg tgggggaaat cgccagatgg ttcatgccac
     5281 tatgatgacg tgacctctca caaagtcgac aaccatggta tatgcgtgaa ggataccact
     5341 gcgggtttcg g
//
LOCUS       DATGEM010000580         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_965398, whole genome shotgun sequence.
ACCESSION   DATGEM010000580 DATGEM010000000
VERSION     DATGEM010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965398"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VKQ27_17700"
     CDS             <1..563
                     /locus_tag="VKQ27_17700"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HLY90821.1"
                     /translation="IRLRDGTVIACNAALVGIGAAPNDALARSAGLTCLNGIVVNQAA
                     RTNDPLIHAVGDCTYRPLPLYQQSARLESVPNALEQAKQAAADLCGRAPPSPEVPWFW
                     SDQFDIRLQIAGLPFDVAELVIRGDPSANSFAIFHLSADGTVQAVEAVNAPAEFMAGR
                     IMVARRRRPLAAQLRDVSCSLKELAG"
     gene            573..899
                     /locus_tag="VKQ27_17705"
     CDS             573..899
                     /locus_tag="VKQ27_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLY90822.1"
                     /translation="MPKVTYVEFNGTNHVADVPVGTSVMRGAVDNNIPGIDADCGGEC
                     ACATCHVYVDAAWLPKVGTPAPGSQEASMLSFAALAEPNSRLSCQISMTDELDGLLVR
                     MPEGQH"
     gene            complement(903..1472)
                     /locus_tag="VKQ27_17710"
     CDS             complement(903..1472)
                     /locus_tag="VKQ27_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90823.1"
                     /translation="MPFDALVAPPRQNALLNALDIHRVAPVSWATLVDHKLAQQEKFG
                     PSFWFRHQTKVSIILIGASPVVGTVVGALQGFNPHSSALAVASSFMWMCLVAMLTGTG
                     LIKLRAGASWQERCVTAFELDELRVPRQIATVARSIQRMVPDAELVLGELKRHEAVLD
                     PYLVLEHRDQRICLGIWEDDKVIAVPTPR"
     gene            1586..3376
                     /locus_tag="VKQ27_17715"
     CDS             1586..3376
                     /locus_tag="VKQ27_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY90824.1"
                     /translation="MADFVDQLADRAGAPIERPLPRPAGNALFGSDVVAETLRALEIP
                     FIALNPGASYRGLHDSLVNYLGNVQPQMLLCLHEEHAVAIAHGYAKVTGKPMAVAVHS
                     NVGLMHGTMAIFNAWCDRMPVLVLGATGPVDAPKRRPWIDWIHTARDQGALIRSYTKW
                     DDQPASPEAAREALLRAYWMMRTAPMGPVYVNLDAGMQEAPLGDPLPPIDAPRFVPSV
                     APAAPTEQIARVLEILHGAQQPVILMGRGSRSEQAWANRVALAEALNARVVTDLKIGA
                     VFPTDHPLHAGAPATFPVQDAAQAIAAADVILSLDWVDLAGALKAACGTVSPSARVIN
                     VTMDHQIHNGWSMDHQALAPADVFMAADADTAIAALLQQLPARDTKRYEPVVAPEPAA
                     EPGHITVAHMGIELRRAVGVRPTSLLHVSLSWNSALWPFRHPLDYIGSDGGGGIGGGP
                     GIAVGAALALRASGRLPLAVCGDGDFLMGATSLWTAVHYNIPLMIVVANNRSFFNDEL
                     HQERVARMRNRPVENRWIGQRIGDPEIDLASLARAQGAVGFGPVTDGADLAHTFRSAI
                     EAVEAGNVAVVDVRVAAGYAPAAVAGLFRG"
     gene            3394..>3883
                     /locus_tag="VKQ27_17720"
     CDS             3394..>3883
                     /locus_tag="VKQ27_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90825.1"
                     /translation="MFRSAERRVEDAEVTWLNRFTAATPGLSSGVIFTPSETSDPYLN
                     DGPPPSLGLQLYFDNIAAMEAALAPDGHLQALPARTSLAGDATQQAMLVRPFPVPDPA
                     FRTPPGKLPCTYLVAYEGQADNLNAWLAYYITSHPPIMARFPGIREIEIYTRIDWCGF
                     LPW"
BASE COUNT          696 a         1250 c         1265 g          672 t
ORIGIN      
        1 gcatccgcct tcgcgatggc acggtcatcg cgtgcaatgc cgcgttggtc ggtatcggtg
       61 cagcaccgaa cgacgccctg gcgcgctcgg ccggattaac atgcctcaac gggatagtcg
      121 tgaatcaggc agcacgtacc aacgatccgc tgattcatgc agtcggcgac tgtacctatc
      181 gacccttgcc cctctaccag caaagcgccc ggctagagag cgtgccgaat gcgctggagc
      241 aggccaagca ggcggccgcg gatttgtgcg ggcgtgcgcc gccgtcgccg gaagtaccct
      301 ggttctggtc cgaccagttc gacatccgcc tccagatcgc cggcctgccg ttcgacgtcg
      361 cggagctggt gatccgcggc gatccatccg ccaattcctt cgccatcttc cacctttcgg
      421 cggacggcac cgtgcaggct gtggaagcag tgaacgcccc cgccgaattc atggccggcc
      481 gcatcatggt ggcgcggcgc agacgccccc tggcggcaca gttgcgggac gtgtcatgct
      541 cgctgaaaga acttgccggc taggggagga cgatgccgaa ggtcacatat gtcgagttca
      601 acggcaccaa tcatgtggcc gacgtgccgg tcggcacatc cgtgatgcgc ggcgccgttg
      661 ataacaatat tccgggcatc gacgccgact gcggcggcga gtgcgcctgc gccacctgtc
      721 acgtctacgt ggacgctgcg tggctgccga aagtaggaac accggcgcct gggtcgcagg
      781 aagccagcat gctgagtttc gccgcgttgg cggagcctaa ttcacgcctc tcgtgccaga
      841 tcagcatgac ggacgagctg gacgggctgc tcgtccgcat gcctgaaggc cagcactgaa
      901 cgtcagcgag gcgtgggaac cgcgatcacc ttgtcgtctt cccagatgcc gaggcagatc
      961 cgctggtccc ggtgctcgag cacgagatag ggatccagca cggcctcgtg ccgcttcaac
     1021 tctcccagca cgagctccgc atcgggaacc atgcgctgga tggagcgggc aactgtggcg
     1081 atctgccggg gaactcgcaa ttcgtccaac tcgaacgcag taacgcaacg ctcctgccag
     1141 gacgcacccg cgcgcagctt gatgagaccg gtgcccgtca gcatcgcgac caggcacatc
     1201 cacatgaagc tgctcgcgac cgccagcgcg ctcgaatgcg ggttgaaacc ctggagcgcg
     1261 ccaacgacag tgccgacaac agggctggct ccaatcagta taatcgacac tttcgtctgg
     1321 tgcctgaacc agaaactggg gccgaacttt tcctgctgcg ccagtttgtg atccaccagc
     1381 gtcgcccatg agacgggcgc gacccgatgg atatccaaag cgttcagaag tgcattttgc
     1441 cgcggtgggg cgaccaaggc atcgaagggc atggctggat ccttcgccga tggagtgctg
     1501 accttcgcta tatggacaac acagcaaccg ggacattaac taggacgcat aacattcgtg
     1561 cgattgcagc acggctgggg gaggcatggc cgatttcgta gaccaactcg ccgatcgagc
     1621 gggcgcgcca atcgaacggc cgttgcctcg gccggcggga aacgcactgt tcggcagtga
     1681 tgtcgtcgca gagacattgc gcgcgctgga gatccccttc atcgccttga atccaggcgc
     1741 cagctatcgg ggattgcacg acagcctggt gaattatctc ggcaacgtgc agccacagat
     1801 gctgctctgc cttcatgaag aacacgcggt tgcgatcgcc catggctacg caaaggtgac
     1861 cggcaagccg atggccgttg ccgttcactc gaatgttggt ctgatgcacg gcacgatggc
     1921 gatcttcaac gcctggtgcg accggatgcc ggtgctggtg cttggcgcga caggaccggt
     1981 cgatgcgccg aagcggcgac cctggatcga ctggatccac accgcgcgcg accagggcgc
     2041 gctgatccgg tcgtatacga agtgggatga ccagccggcc tcacccgagg cggcgcggga
     2101 ggctttgctg cgcgcgtatt ggatgatgcg cacagcaccc atggggccgg tttatgtgaa
     2161 cctcgatgcg ggcatgcagg aagcgccgct cggcgatccc ctgccgccga tcgatgcgcc
     2221 acgtttcgtg ccatccgtcg cgcccgcggc gccaacagag cagatcgcgc gtgttctgga
     2281 gatactccac ggtgcgcagc agccggtcat cttgatgggg cgaggttcgc gcagtgagca
     2341 ggcgtgggcg aaccgcgtgg cgctcgccga ggccttgaac gcgcgcgtag taacggatct
     2401 gaagatcggc gcggtgtttc cgaccgatca cccgctgcat gccggtgctc ctgcgacgtt
     2461 cccggtgcag gacgcggctc aggcgatcgc ggcggcggac gtgatcctca gtttggattg
     2521 ggtggacctg gccggagcgc tgaaggcggc gtgtggaacc gtgtcgccca gcgcgcgggt
     2581 catcaacgtg accatggacc accagatcca caacggctgg agcatggatc atcaggcgct
     2641 ggcaccagcc gatgtgttca tggcagccga cgcggacacc gcgatagcgg cgctgctgca
     2701 gcaattgccg gcccgcgata caaaacgata cgaacccgtc gtcgcacccg aaccggcggc
     2761 ggaaccgggg cacatcaccg tggcacacat gggaatcgag ctgcggcggg cggttggcgt
     2821 acgcccaaca tcgctgctgc acgtgtcgct ctcgtggaat tcggctttgt ggccgttccg
     2881 gcatccgctc gactatatcg gttcggacgg aggtggcggc atcggcggag gtccgggcat
     2941 cgccgttggc gcagctctgg cattgcgagc gagcggcagg ctgcccctgg cggtgtgtgg
     3001 tgatggcgac ttcctgatgg gcgccacatc gctctggacg gccgtgcact acaacatccc
     3061 gctgatgatc gtggtcgcca acaaccgctc gttcttcaac gacgaactgc accaggagcg
     3121 cgtagcccgg atgcgtaacc ggccggtgga aaatcgctgg atcggccagc gcatcggcga
     3181 tccggagatc gatctggcga gcctcgcgcg cgcgcagggc gcggtcggat tcggtcccgt
     3241 cacggatggc gccgatctcg cgcacacctt ccgcagcgcc atcgaggctg tcgaagcagg
     3301 caacgttgcc gtcgtcgacg tgcgcgtggc ggcgggctat gcgccggccg ccgtcgccgg
     3361 tctgttccgc ggatgagggt gtgctggttt gccttgttcc ggagtgccga gcgacgtgtc
     3421 gaggacgcgg aggtaacctg gctgaaccgc ttcaccgcag ccacgcccgg cctgtcgagc
     3481 ggcgtcatct tcacgccgtc ggagaccagc gatccgtatc tcaacgacgg gccgccaccc
     3541 tccctcggac tgcaactcta cttcgacaat atcgccgcga tggaagcggc cctggcgccg
     3601 gacgggcact tacaggcgct ccccgcgcgc acctccctgg caggagatgc gacgcagcag
     3661 gcaatgctgg tgcggccctt cccggtgccg gacccggcat tccgcacccc gccaggaaaa
     3721 ctgccctgca cgtatctcgt ggcttacgaa ggacaggcgg acaacctgaa tgcctggctc
     3781 gcctactata tcaccagcca tccgccgatc atggcgcgat ttcccggcat ccgggagatc
     3841 gagatataca cccgtatcga ctggtgcggc ttcctgccct ggc
//
LOCUS       DATGEM010000581         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1145170, whole genome shotgun sequence.
ACCESSION   DATGEM010000581 DATGEM010000000
VERSION     DATGEM010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145170"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VKQ27_17725"
     CDS             complement(<1..503)
                     /locus_tag="VKQ27_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90826.1"
                     /translation="MNRARTLRASRWRACMTLAVLAGALALGGCLADEGYEMPSRAMK
                     ELSPQMLAQLQAKNMPKESPILVRIFKEESELEVWKQDNSGRYALLKVYPICRWSGDI
                     GPKIREGDRQAPEGFYAITPGLMNPNSNYYLAINTGFPNAYDRANNRHGAFLMIHGDC
                     SSRGCYA"
     gene            complement(762..1718)
                     /locus_tag="VKQ27_17730"
     CDS             complement(762..1718)
                     /locus_tag="VKQ27_17730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HLY90827.1"
                     /translation="MRSYLDFEKPVAELEAKIEELRALQEEGDAIAIGEEIGRIEAKA
                     AQALEDLYGALTPWQKTQVARHPQRPHCLDYIAALIADFTPLAGDRKHGEDAAIIGGF
                     GRFAGQSVCVIGHEKGSYTENRLKHNFGMARPEGYRKAVRLMHLADRFDIPVIALIDT
                     AGAYPGIGAEERGQAEAIARSTEACLEIGVPNVAAIIGEGGSGGAVAIATANRVIMLE
                     HAIYSVISPEGAASILWRDTARAQDAAAAMKITAQDLARLGVIDAIVPEPVGGAHREP
                     AAAIAATGEAVAAALADLRGLDRATIMRLRREKFTAIGRTLS"
     gene            2199..>4690
                     /locus_tag="VKQ27_17735"
     CDS             2199..>4690
                     /locus_tag="VKQ27_17735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HLY90828.1"
                     /translation="MDINRLPPGVIPEHYDLFIRPDPGFFDVSSEPVLRFEGSVSIKI
                     LVDQPTAEVTLNAAELQLRHAILDGDERATISLNEREQTATLVFDRPIAVGTHALSID
                     YSGKIYDGAQGLFVSQYKTAQGSRRLLVTQFEPGDARRFIPCWDEPARKATFTVAVAA
                     PKDMLAVSNMPIEVVSELDEERVYVRFQKTPGMSSYLLFLAIGDLERVSMRSGATVVS
                     LVAKTGSAQDGRFALESAVKLLEFYNEYFGVPFPLPKLDMIAAPGAGGFSAMENWGAI
                     LYFEDQLLLNPEWSTESNRQRVFVVVAHEMAHQWFGDLVTMHWWDDLWLNEGFASWME
                     RKATDHFNKDWNNWLDAEFETQRAMRQDSMKATHPVVKRVLKVQEAGFDDITYRKGRA
                     VIRMIENYIGEDAFREGIRAYMKRYAHQNAVTDNLWDELEAASNKKIKELAGDFINQP
                     GVPLIAVESVAADGYKIRQKRFAVDANPNDTGEWTIPVYALSAGQVQNVPVLVQKGES
                     NATAIPASPPRKLNAGQTVYYRVDYGSAFAELLNEFGALKAPDQLGLLNDAWALGEAD
                     AAPIGNYLDLTLKLRTDTDPLVWRQLADTFVAIDLLYDSADPRRNSWRAYGRRMLNPA
                     LAAIKWDKQSGEPNNKSILRESLITALAYLDDSGVKKEARHRFDDYRAGKPLPALIKR
                     PVFQAVCLGADTGLYTQVYDLAKKATDSFAQDQMFVALALAGEPLLAQESLKTALGKD
                     PAKTTGPKMIQSVAVRNPDLAWQFALINLETINKLLDAEQRGRFIPSLTATSRSQQVL
                     NELKDIRKKDPEISPSWFAKSVAELEFRLEVV"
BASE COUNT          953 a         1425 c         1452 g          860 t
ORIGIN      
        1 atggcgtagc agccgcgcga cgagcagtcg ccgtgaatca tcaggaacgc gccgtgccgg
       61 ttattggcgc gatcgtaggc gttggggaaa ccggtattga tggcgaggta gtagttggaa
      121 ttcggattca tcaggccggg cgtgatggca taaaagcctt cgggcgcctg acggtcgccc
      181 tccctgatct tcggcccgat gtcgcccgac cagcggcaga tcggatagac tttgaggagc
      241 gcgtagcggc cgctgttgtc ctgcttccag acttcgagct ccgactcctc cttgaagata
      301 cggacgagga tcggcgactc cttgggcatg ttcttggcct ggagctgcgc cagcatctgc
      361 ggcgacaatt ccttcatcgc ccgcgacggc atctcgtagc cctcgtcggc aagacagccg
      421 ccgagcgcca acgcgccggc cagaaccgcc aacgtcatgc acgcgcgcca acgcgacgcg
      481 cgaagggtgc gggcgcggtt caatgcagga ctccccgacc gatggaccat ccccatgcca
      541 tcaccgccca tggttacccc caaggggtgg ctttcgcaag accgagcggc gccgcgcagg
      601 gtggctccgt gggtgataac gaacggtaaa ctttcgccaa atttgctggg cgaattcaac
      661 gcgctagcaa acactgacgg caggctcgcc gtcgctggtg cgcgccgcag tggatcgatc
      721 ccggcaaaat cgtggcacaa gcgtggcggc gcgaccgaag ctcaggacag cgtgcgtccg
      781 atcgcggtga atttctcgcg gcgcaaccgc atgatggtgg cgcgatcgag cccgcgcagg
      841 tcggcgagtg cggccgcgac cgcctcaccg gtcgccgcga tggcagcggc gggctcgcgg
      901 tgcgcgccgc cgaccggctc cggcacgatg gcgtcgatga cgccaagccg cgccaaatct
      961 tgcgcggtga tcttcatcgc cgcggcggcg tcctgcgccc gcgccgtgtc gcgccatagg
     1021 atcgaggcgg cgccttccgg cgagatcacg ctgtaaatgg cgtgctcgag catgatgacg
     1081 cggttcgcgg tggcaatcgc caccgcgccg ccggagccgc cctcgccgat gatcgccgcc
     1141 acgttgggca cgccgatttc gaggcaggct tcggtcgagc gggcgatggc ctcggcttgg
     1201 ccgcgctcct cggcgccaat gcccggatag gcgccggcgg tgtcgatcag cgcgatgacc
     1261 ggaatgtcga agcgatcggc caaatgcatc aggcggacgg ccttgcggta gccttccggc
     1321 cgcgccatgc cgaaattgtg cttgagccgg ttctcggtgt aggatccctt ttcgtggccg
     1381 atcacgcaca cactctggcc cgcgaaacgg ccaaagccgc cgatgatcgc cgcgtcctcg
     1441 ccatgcttgc ggtccccggc gagcggcgtg aaatcggcga tcagtgccgc gatatagtcg
     1501 agacaatgcg gccgctgcgg gtggcgcgcc acctgggtct tctgccacgg cgtcagcgcg
     1561 ccgtagaggt cctcgagcgc ctgggcggcc ttggcctcga tacggccgat ctcctcgcct
     1621 atggcaatgg cgtcgccctc ctcctgcagg gcgcgcagtt cctcgatttt ggcctccaat
     1681 tcggccaccg gcttttcgaa gtcgagataa gagcgcatca ggcgagaaat ctaagaccta
     1741 tcaagtcctg aggacagatg ccggctcctg tcgcgaacgc aggatatctt ggactgaact
     1801 tgcggctgaa attgcgttta ggccgatttt tatctacctc tggttgaccc taacaacgaa
     1861 aatcattaat aatcgacttc tgaagatgat ctcatgctcg gttgcggagc cgcgaaccat
     1921 gagagcggcg cgcgccgatc ttcccggctt cagaactacg cgcgagcacg acggcgtgtg
     1981 ttgccggtgc ctgatacgac ctcgaccgca gcaagatttc acgcctgagg agccgaccca
     2041 tgtcggtcat tgacgatcag gcactatcgc gccggaaggt tttgggcggc agcgcttcgt
     2101 tggcgttggg cgcgactctt gccggccttg gtagcggcca agtccgacca gcgcacgccc
     2161 agtcgccggc agcgaccagc aaacaaaggg ggaggtccat ggatatcaac cgcctgccgc
     2221 cgggcgtcat ccccgagcac tacgatttgt tcatccgacc cgatccggga ttctttgacg
     2281 tctcctccga accggttctc cggttcgaag gttcggtttc gataaagatc ctggtggacc
     2341 agccgaccgc cgaagtgacg ttgaacgcgg cagagctgca attgcgccac gccatcctgg
     2401 acggggatga gagagcgacg atttcgctca acgaacgcga gcagacggcc acgttggtgt
     2461 tcgatcgccc gatcgccgtt ggtacgcatg cgctgtcgat cgattacagc ggcaagatct
     2521 atgacggcgc ccaaggcctg ttcgtctcgc aatacaagac cgcgcaaggc tctaggcgcc
     2581 tgctcgtgac ccagttcgag cccggtgatg cccgacgctt cattccttgc tgggacgaac
     2641 cggccagaaa ggcgacgttc acggtcgcag tcgcggctcc caaagacatg ttggccgtct
     2701 caaacatgcc catcgaggta gtgtccgagc tcgacgaaga acgtgtgtat gtccggttcc
     2761 aaaagacccc agggatgtca tcgtatcttc tgttcttggc catcggggat ttggaacgag
     2821 tgagtatgcg cagcggggca accgtcgtct ctctcgtggc caagacggga agcgcgcaag
     2881 acggaaggtt cgcgctcgaa agcgcggtca aactgctcga attctacaac gaatatttcg
     2941 gtgttccctt cccgctcccc aagctcgaca tgatcgcagc gcccggagcc ggcggtttct
     3001 cggcgatgga gaactggggc gcgatcctct atttcgagga tcagctgctc ttgaacccgg
     3061 aatggtcgac cgaatcgaat cgccagcgag tcttcgtcgt cgtcgcccac gagatggcac
     3121 atcaatggtt cggggacctg gtgacgatgc attggtggga cgatttgtgg ctcaacgaag
     3181 gcttcgcgtc gtggatggag cgcaaggcga cggatcattt caacaaagat tggaataact
     3241 ggcttgacgc ggaattcgaa acgcagcgag cgatgcgtca ggactcgatg aaagcgaccc
     3301 acccggtggt caagcgagtc ctgaaagtcc aagaggcggg tttcgacgac atcacctatc
     3361 gcaagggccg agcggttatc cggatgatcg aaaattacat cggcgaggac gcgtttcgcg
     3421 agggcatccg ggcctacatg aaacgatacg cccaccaaaa cgcggttacg gacaatctct
     3481 gggacgaatt ggaggcagca tccaacaaga agatcaagga gctggccggc gacttcatca
     3541 accagcctgg ggtcccgctg attgcggtcg agagcgttgc ggccgacggc tacaaaatcc
     3601 ggcagaaacg attcgcggtc gatgccaatc cgaacgacac aggcgagtgg acgatcccgg
     3661 tctacgcgct ttcggcggga caggtgcaga atgttcccgt gctggtgcaa aaaggcgaga
     3721 gcaacgccac ggcgataccc gcgtctccgc cgagaaagct caacgccggc cagaccgtgt
     3781 attaccgtgt cgactacggt agcgccttcg ccgagctgtt aaatgaattc ggggcgctta
     3841 aagctccgga ccaacttggg ctcctgaacg atgcctgggc gttgggcgaa gcggacgcgg
     3901 caccgatcgg caactatctc gatctgacgc tgaagctgag gaccgacact gatcccctcg
     3961 tctggcgtca actcgccgat acgttcgtcg ctattgattt gctctatgat tccgccgatc
     4021 cgcgacggaa ttcgtggcga gcctatggcc gcaggatgct gaacccggcg ctggcggcga
     4081 taaaatggga caagcaatca ggcgagccga acaacaagag tatcttgcgc gaaagcctga
     4141 ttaccgctct ggcttatttg gacgacagcg gcgtcaagaa ggaggcccgc caccgcttcg
     4201 acgattatcg agccggcaag cccctgccag cgctcatcaa acggccggtt ttccaagccg
     4261 tgtgcctcgg cgccgacaca ggtctataca cacaggtata cgacctcgct aagaaagcga
     4321 cggactcgtt cgcccaggac cagatgttcg ttgctctcgc cttggccggc gagccgctgc
     4381 tcgctcagga atcgcttaaa actgcgctcg gcaaggatcc agcgaaaacg accgggccaa
     4441 agatgatcca aagcgtcgcc gtgcgcaatc ctgatctggc ctggcaattc gccctcataa
     4501 atcttgagac gattaacaag ctgctggatg cagagcagag aggtagattc ataccaagcc
     4561 tcacggcgac atcgcgtagc cagcaagtgc tcaacgaact gaaagacatc agaaagaaag
     4621 acccggaaat ctcccccagc tggttcgcaa aaagcgttgc agagctggaa ttccggcttg
     4681 aggtggtgca
//
LOCUS       DATGEM010000582         5378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_385206, whole genome shotgun sequence.
ACCESSION   DATGEM010000582 DATGEM010000000
VERSION     DATGEM010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385206"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VKQ27_17740"
     CDS             complement(<1..244)
                     /locus_tag="VKQ27_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90829.1"
                     /translation="MDMQVDRATSTLYDKLEERVLARDQVGASSVYYDLVRAGRPLNE
                     IMAAGVRIHAPYTHVPYHERIDDGYPNFVNNDHCLLS"
     gene            333..1265
                     /locus_tag="VKQ27_17745"
     CDS             333..1265
                     /locus_tag="VKQ27_17745"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90830.1"
                     /translation="MPWTMSVVAGSGEQGYAGDGGPAAQALLNNPFDLAFDPAGNLCF
                     SDTYNHCIRRIDARSGIISTIAGIGQAGFAGDDGSATQAKMNQPYGIVIDRAGTIYVA
                     DRLNKRVRRIDGSSGIISTMAVLEEPNGLALDRNHRRLFIADVADHRVQVIDLSSGAI
                     SVFAGTGEARHDGDGGSATSAGVFGARAVALAPDDSLYILERQGSCLRRVRGAVIETI
                     AGTGARGYAGDDHDARYAVFDAPKEMAIDPAGNVFIVDTENHVIRLIDARTWIVTTIC
                     TGLARPHGAVVGADGAVYIGDSEHHQVRKLVRTS"
     gene            complement(1335..2108)
                     /locus_tag="VKQ27_17750"
     CDS             complement(1335..2108)
                     /locus_tag="VKQ27_17750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY90831.1"
                     /translation="MPGRDRWRRRLLPLIGGATLLFIWWALIAIFHVRSFIAPSPDVV
                     VTTLYNKFAMLMANLAPTALEALCGFAVGNTAAIFVATVFVHRKSVEQAFFPIAVLLH
                     SIPVVATAPILVLLLGNGLAPKVAIAAIICFFPTLVNMVRGLESVSPQSMELMHVLSA
                     TKWEVFFKLRVPTSLPYLFSALRIAASTATIGAIVGEWIGSQVGIGALIIQATYNFDS
                     ALLYATVLCGSCFSVVFFQVIRLIERKVVRWDTGAATTT"
     gene            complement(2108..2881)
                     /locus_tag="VKQ27_17755"
     CDS             complement(2108..2881)
                     /locus_tag="VKQ27_17755"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90832.1"
                     /translation="MATTPAVELRDVTVTFTNDRRAVTALERISLTVAEGSFLTLLGP
                     SGCGKSTLLRVIADIVQPTAGSVRVLGETPAEARRNRHIGFVFQDAALLPWRSALDNI
                     RLPLEVGGRRTLPEGAMGPEELLSLVGLADWKNALPHELSGGMRQRVSIARALLGGPR
                     VLLMDEPFGALDEITRDRLNEELLRIWRETGTTIVFVTHSIYEAAFLGQQVLMMAAHP
                     GRVREVVSIQLPEPRNVSMRETTGFIEQAAHLRAVLENC"
     gene            complement(2881..3900)
                     /locus_tag="VKQ27_17760"
     CDS             complement(2881..3900)
                     /locus_tag="VKQ27_17760"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90833.1"
                     /translation="MRSLANLNRRSLLRASAVVGATLLLTDQASAETATINMQLGWLG
                     GGNQLGEACAHQLGYFKDEGVDFHFQPGGPNNDGIAVVASGRYELGEVSSSPSLMLAV
                     SQDIPIRCFAVSAQKHPYTFFSLKKNPVRTPVDFVGKKVGIQATGVILLRALLAKNKI
                     DMKDVQVVTIGSDMMPLLTGQVDVVTGWLTNTTALKVLGADRVDLPLWDTGVHLYADP
                     YYATTDTLTGHKDLLVRFLRAASRGWAFAYENRDKAIELLVKEFPNLNAADERAAADV
                     MLSYCFNANTKLNGYATMDPQVWQDQIDTWSQLGQFTKRTPKVDDVMTMDILNATQDS
                     RPKVG"
     gene            complement(3987..5099)
                     /locus_tag="VKQ27_17765"
     CDS             complement(3987..5099)
                     /locus_tag="VKQ27_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLY90834.1"
                     /translation="MAPPSFPPGSQVEVVRELPLDQIMLGDCVQLMRMLPPASVHCVF
                     ADPPYNLQLRGELRRPDDSLVDGVDEDWDRFSDFATYDAFTREWLTECRRLLRKDGTL
                     WVIGAYHNIFRIGAILQDLGFWMLNDVIWRKSNPMPNFRGRRFTNAHETLIWAARGRD
                     SRYRFNYAGMKALNDDIQMRSDWWIPLCTGAERLRNQHGLKLHPTQKPEALLHRVLLA
                     STQPGDIVLDPFAGTGTTAAVAKRLHRHFIGIERHPAYAEAAIGRVRATRPAPDEGLS
                     GAVSKRDAPRVPFGSLVERGLLAPGTKLFDRMRRVSAVVTVDGTVVAGPHRGSIHKVG
                     AAVQNAPACNGWTFWHFERDGRLVALDALRDEAVGP"
     gene            complement(5144..>5378)
                     /locus_tag="VKQ27_17770"
     CDS             complement(5144..>5378)
                     /locus_tag="VKQ27_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLY90835.1"
                     /translation="VGGDGRSLSIAAASIVAKVLRDRAMTRLGVRFPDYGWHSNAGYA
                     TEFHVSAIRRLGPTRHHRPSFGTVAQMTLDLLN"
BASE COUNT          992 a         1679 c         1738 g          969 t
ORIGIN      
        1 cgctcagcag acagtgatcg ttgttgacaa aattgggata gccgtcatcg atacgctcgt
       61 gatacggcac gtgcgtgtaa ggtgcgtgga tgcgcacacc ggcggccata atttcattca
      121 gtggacggcc tgcgcgcacg aggtcatagt acacactgct cgcgcccacc tgatcgcgcg
      181 ccagcacacg ctcctcgagc ttgtcataaa gtgtgctcgt ggcgcgatcg acttgcatgt
      241 ccatccttgg catcctccgg ctagttagcc aatcctacgc ttggctggcc agtttcgaca
      301 accttggcgg ccataacggg aggaaccaca ccatgccctg gacgatgagc gtagtggccg
      361 ggagcggcga acaaggttac gccggcgatg gtggaccggc ggcgcaggcg ctgctgaaca
      421 atccgttcga cctcgcattc gatcctgcgg ggaacttgtg cttctccgat acctataatc
      481 actgcatccg gcggatcgat gcgcggtcgg ggatcatttc gacaatcgcc gggatcggcc
      541 aggccggatt tgctggtgac gatggctccg cgacgcaggc gaagatgaac cagccatatg
      601 gcatcgtcat cgatcgagcg ggcacaattt atgtcgccga tcgattgaac aagcgggtcc
      661 ggcgtataga cggcagcagt ggcatcatct cgacgatggc cgtgctggag gaaccgaacg
      721 gactggcgct cgatcgcaac caccggcggc tgttcatcgc ggatgtggcg gatcaccggg
      781 tgcaggtgat cgacctgtcg tcgggcgcca tcagcgtgtt tgccggcacc ggtgaggccc
      841 ggcacgacgg cgacggcggt tcggcaacct ctgccggcgt gttcggcgcg cgtgcggtgg
      901 cgctggcgcc ggacgattcg ctgtatatac tggagcgcca gggcagctgc ctgcggcggg
      961 tacgtggcgc ggtgatcgag acgatcgcgg gcacaggcgc gcggggctac gccggcgatg
     1021 atcatgatgc gcgctatgcc gtgttcgatg cgccgaagga gatggcgatc gatccagccg
     1081 gcaatgtatt catcgtcgat accgaaaatc acgtgatccg gctgattgac gcccgcacct
     1141 ggatcgtgac cacgatctgt accggcctcg cgcgtcccca cggcgctgtc gtgggcgctg
     1201 atggcgctgt ttatatcggt gattcggaac accaccaggt tcgcaagctg gtgcggacca
     1261 gctaggatcc acgatcggaa gaagctgcgc tgccagcgca cattgactgc attgtccttc
     1321 gatggctctg aagattatgt ggtcgtggca gcgccggtgt cccatcgcac caccttacgc
     1381 tcgatcaagc ggatgacctg gaaaaacacc accgagaagc atgatccgca caacacggtc
     1441 gcgtagagca atgccgagtc gaagttgtag gtggcctgga tgatcagcgc gccaatcccg
     1501 acctggctgc caatccattc gccgacgatg gcgccaatcg tcgctgtgct ggcggcgatg
     1561 cgcagcgcgg agaacagata gggcagggac gtcggaacac gcagcttgaa gaacacctcc
     1621 cacttcgtcg ctgacaggac gtgcatcagc tccatcgatt gcgggctgac cgattcaagc
     1681 cctcgcacca tgttcaccaa tgtcgggaag aagcagatga tcgcggcgat cgccactttc
     1741 ggcgccaggc cattgcccag cagaagcacc aggatcggcg ctgtcgccac caccgggatc
     1801 gaatgcagca gcacggcgat cggaaagaag gcctgctcga cgctcttgcg atgcacgaac
     1861 acggtcgcga cgaaaatcgc ggctgtgttg ccgacagcga acccgcacag cgcctccaaa
     1921 gccgtgggag ccaggttcgc catcagcatg gcgaacttgt tgtacagcgt ggtcaccacg
     1981 acgtctggtg atggcgcgat gaacgaacgc acgtgaaaga tggcgatcaa cgcccaccag
     2041 atgaacagaa gcgtcgcgcc accaatcagc ggcaacagtc gccggcgcca ccggtcgcga
     2101 cccggcatca acagttttcc agcactgcgc gcaggtgggc ggcctgttca atgaacccag
     2161 tcgtttcccg catcgacacg ttgcgcggct cgggcagttg gatcgagacg acttcccgca
     2221 cgcggccggg atgcgcggcc atcatcagca cctgctggcc gaggaatgct gcctcataga
     2281 tgctgtgagt tacgaagacg atcgtcgtgc ccgtctcgcg ccagatgcgc agcagctcct
     2341 cgttcaggcg atcgcgcgtg atttcatcca gcgcgccgaa tggttcgtcc atcagcagca
     2401 cgcgcgggcc gcccaggagt gcgcgagcga tcgacacgcg ctggcgcatg ccgccagaaa
     2461 gctcgtgcgg cagcgcattc ttccagtcgg cgaggccgac aagcgacagc aattcctccg
     2521 gtcccatcgc gccttcgggc aacgtgcgcc gcccgcccac ttcgagtggc aggcggatgt
     2581 tgtcgagcgc gctgcgccac ggcagcaggg ccgcgtcctg gaagacgaag ccgatgtggc
     2641 gattgcgccg tgcttccgcc ggtgtctcgc caagtacgcg cacgctgccg gctgtcggct
     2701 gcacgatatc ggcgatcacg cgcagcaggg tcgatttacc gcagccggat gggccgagca
     2761 gcgtcaggaa gctgccctcc gcgaccgtca atgagatccg ttccagcgcg gttaccgcgc
     2821 gccggtcgtt ggtgaaggtg acggtgacgt cgcggagttc gacggccggc gtagtggcca
     2881 tcaaccgacc ttgggtcggc tgtcctgggt tgcgttcagg atatccatcg tcatcacgtc
     2941 atcgaccttc ggcgtccgct tggtgaactg gccaagctgg ctccaggtgt cgatctggtc
     3001 ctgccagacc tgcggatcca tcgttgcgta gccgttcagc tttgtattgg cattgaagca
     3061 gtaggacagc atcacgtcgg ctgcggcgcg ttcgtcggcg gcattgagat tggggaactc
     3121 cttcaccagc agctcgatcg ccttgtcgcg gttctcgtag gcaaaagccc agccgcgcga
     3181 tgccgcacgc aggaaccgca ccagcaggtc cttatggccg gtcagcgtgt cggtcgtggc
     3241 atagtatgga tcagcgtata gatgcacgcc ggtatcccac agcggcaggt ccacgcgatc
     3301 ggcgccgagc accttcagcg cggtggtgtt ggtgagccag ccggtgacca cgtctacctg
     3361 tccggtcagc agcggcatca tgtcggagcc gatggtgacg acttgcacat ctttcatgtc
     3421 gatcttgttc ttggcgagca gcgcgcgcag caggatcacc ccggtggcct ggataccgac
     3481 tttcttgccg acgaaatcca ccggtgtgcg cacgggattc ttcttcagtg agaagaatgt
     3541 gtatgggtgc ttctgtgctg acacagcgaa gcagcggatc gggatgtcct gcgagacggc
     3601 gagcatcaat gaggggctgg atgacacctc tcccagctcg tagcggccgg acgcgacgac
     3661 agcaatgccg tcgttattcg gtccaccggg ctggaagtgg aagtccactc cctcgtcctt
     3721 gaagtagcca agctggtgcg cacaggcttc gccaagctgg ttgccgccgc ccagccagcc
     3781 gagctgcatg ttgatcgtgg cggtttcagc ggaggcctgg tctgtcagca gaagcgtggc
     3841 gccgacaacg gcactcgccc gcaataggga gcgacggttc aggttcgcga gtgaacgcat
     3901 ggttaggccc cctggttgtt tgtgttacga gttgaataac aggggtatcg ccggccacgc
     3961 aattcgccga ccttatatcc acatatctac ggtccaacgg cctcgtcgcg gagggcatcc
     4021 agcgcgacga gccggccgtc ccgttcgaag tgccagaatg tccagccatt gcaggccggc
     4081 gcgttctgca ccgccgcgcc gaccttatgg atcgagccac ggtgcggtcc ggcaaccaca
     4141 gttccatcca cggtcaccac ggcactgacg cgccgcatac ggtcgaacag cttcgtgcct
     4201 ggtgccagca ggccgcgctc gaccaggctg ccgaacggca cgcgcggtgc gtcgcgtttc
     4261 gacactgcac ccgacaatcc ctcgtctggg gcgggcctgg tcgcacggac tcgcccgatc
     4321 gccgcctcgg cataggcggg atgccgctca atcccgatga agtgccggtg cagtcgcttc
     4381 gccactgcgg cggtggtgcc ggtgccggca aaagggtcca gcacgatatc gcccggctga
     4441 gtggaggcca gcagcacgcg gtgcagcaac gcctccggct tttgcgtcgg atgcagcttc
     4501 agcccgtgct ggttgcgcag ccgctccgca ccagtgcaca gcgggatcca ccagtcgctg
     4561 cgcatctgaa tgtcgtcgtt cagtgctttc atgccggcat agttgaaccg gtaacgcgag
     4621 tccctcccgc gcgccgccca aatcaaagtc tcgtgcgcat tggtgaaccg gcgcccacgg
     4681 aaattgggca tcgggttgga cttgcgccag atcacgtcgt tcagcatcca gaagccgaga
     4741 tcctgcagga tcgcgccgat gcggaagatg ttgtggtagg cgccgatgac ccacagcgtg
     4801 ccgtccttgc gcagcaggcg gcggcactca gtcagccact cgcgcgtgaa tgcgtcgtag
     4861 gtggcgaagt cggagaagcg gtcccagtcc tcgtccacgc cgtcgacaag gctgtcgtcc
     4921 ggccgccgta gctcgccgcg cagttgcagg ttgtagggcg ggtcggcgaa cacgcaatga
     4981 accgaggccg gcggcagcat gcgcatgagc tgcacgcagt cccccagcat gatctgatcg
     5041 agcggcagtt cgcggacgac ttccacttgg ctcccgggcg ggaaggatgg cggcgccatg
     5101 gtggcggccg tctcgaagcc gcgtcaattg ttgtaagcgg gcatcagttc agaaggtcca
     5161 gcgtcatctg ggcgaccgtg ccaaagctcg gcctgtgatg ccgggtcggg cccagcctgc
     5221 ggatcgccga cacgtggaac tcggtcgcat agccggcatt gctgtgccag ccgtagtcgg
     5281 ggaaccgcac cccgagccgc gtcatcgcgc ggtcacgcag caccttggca acgatggagg
     5341 cggcggcgat cgacagcgac cggccgtcac cgcctacc
//
LOCUS       DATGEM010000583         7745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_501766, whole genome shotgun sequence.
ACCESSION   DATGEM010000583 DATGEM010000000
VERSION     DATGEM010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501766"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VKQ27_17775"
     CDS             <1..401
                     /locus_tag="VKQ27_17775"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HLY90836.1"
                     /translation="HTGPDVHPNMTATAAQCSAWYRADMTAKMKAVLAVSPELAGNRN
                     ALMAAGDLAYNSGAGKWPGSPMRAAFAKGQWAAGCQAFAGYMTGYKAPKPVPGRRCWI
                     SKLNGKLYCELPGLVVRRKGERDLCLGLPA"
     gene            398..751
                     /locus_tag="VKQ27_17780"
     CDS             398..751
                     /locus_tag="VKQ27_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90837.1"
                     /translation="MRLVDLDPRWLMKDGARIGFVFRSPTLGDDMWMTCFEAPPPLRE
                     LWPMVKDAFPEGPANEKGFTRSIQPMNQAQRWQIAGGIDAASFETMTVTPSLDGSPGG
                     NWHGFITNGQIVGGI"
     gene            751..1179
                     /locus_tag="VKQ27_17785"
     CDS             751..1179
                     /locus_tag="VKQ27_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90838.1"
                     /translation="MGEWHHPEHYSEQAKGFARAMRVAEIDVFVQAALPTLIGAARHH
                     GYALARHGSEARDLDLIAIPWTESASDADSLIEALCEATRQATGWGHWSHRGHRGERE
                     QKPHGRVAVMILATAEVHIDLSVMPRVPAGLDAVIPDHLR"
     gene            1191..1628
                     /locus_tag="VKQ27_17790"
     CDS             1191..1628
                     /locus_tag="VKQ27_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90839.1"
                     /translation="MKMLTRAAAVLLALVACATPALAQHATFSYAPAGPAPEVAPIVV
                     AATSTPLTATPAAGTTVSGPFTPQLGRDLRIIIKGTWAGTISVGTSVDNCATVNPLTA
                     GGATWGSFTANANEVVDTPTLAGVGYCVTSVITSGTPTIGLRQ"
     gene            1640..2926
                     /locus_tag="VKQ27_17795"
     CDS             1640..2926
                     /locus_tag="VKQ27_17795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLY90840.1"
                     /translation="MRALTALLILVGLGSSPAWASPDPTARALAIQASAAATAAVGPA
                     YTGAASVREIGNRVAIANQKSGSQTRAGFRTWEVDYVGGNPKVCFSNFIVNSTVSGVE
                     TATGGAMNLKLYVELNGVVTPFTWGGAATSSIASGGYGCTDPSPVVIPPYTKYRLCGD
                     MQMGSGGTIPSEGWSNSADLPGGDEYQVGTTSDFCASNTKLATSAAAAIMPALIIAPS
                     SLSVWGVVGDSITAGVNYTQQQPSGGRGILAALAEYGPALNYGVPGDKATWYATNSTV
                     RRQLLALGGVTALVDELGVNDLTGSRTAAQLLADRATIRGLFPGVTIYETTIMPTTST
                     TDGYKTAANQAVAGSSAQRTSFNDTERGGVAGVASLLDFAAEVETSTTTEQGFVLDGG
                     VWTASCMVGSDGTHPVEQCERSRLAPLVRALRSNRR"
     gene            3304..4020
                     /locus_tag="VKQ27_17800"
     CDS             3304..4020
                     /locus_tag="VKQ27_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90841.1"
                     /translation="MNITPLAPRAGAAAARLVAWAQQRASEQSTKTGLVVLAGVLGWH
                     LSTGQAGEIANALQMVLGLLGTALAAATTSSPHPPAQTAPADPQPAAPVPLAHEDQAN
                     ILIPDNPNVLQETNMTTILAVLEADAIAILKAAGKAVESFFLTETQKFVAQAKQTYLG
                     TLGLNLIQTMESQSLTGEQKMETVVAMLVPAIEKFVASGGLPSLVGSVEDFAREFAQS
                     LFNDFAADVAKIAAPTQAAA"
     gene            4017..4280
                     /locus_tag="VKQ27_17805"
     CDS             4017..4280
                     /locus_tag="VKQ27_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90842.1"
                     /translation="MNARVCRVIATAPQVTVPETGRPAAQVLTDNAWHAESGIYLELG
                     SPLPDLGTVITWEGHWVSWTDATGQQVKARKVEYAFDPDATLH"
     gene            complement(4274..4885)
                     /locus_tag="VKQ27_17810"
     CDS             complement(4274..4885)
                     /locus_tag="VKQ27_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HLY90843.1"
                     /translation="MLSGDTHRVIGEYSFLKFLKLYARNRSFRPLVPIRLYQRLRGSA
                     LGQMAYPILWLWHRRATRRAGMEMPLRTDIGPAFHISHGFGVIINEHARIGANCTIMH
                     GATLGRADRLDGESRTIGGCPMIEDDVWIGPHAIIVGAVTIGAGSRILGGAVVTSDIP
                     PRSMVAGNPGAVIKSNCARDVLNPTPIDTKYRRTHRPSALAHQ"
     gene            5121..5327
                     /locus_tag="VKQ27_17815"
     CDS             5121..5327
                     /locus_tag="VKQ27_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90844.1"
                     /translation="MTLQNMRENGATSVEAWCACGHHGPVDVTGWPDDMEVPNVKWRV
                     RCSKCGQRPKQTVPAWPARRGLLD"
     gene            complement(5324..5551)
                     /locus_tag="VKQ27_17820"
     CDS             complement(5324..5551)
                     /locus_tag="VKQ27_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90845.1"
                     /translation="MIFVKGIDWQPIANIPDDRKDGREILLWQGGDAPGPDIGTWNPS
                     EEWGTDGFWEALYEGAPISDVTHWADINPPA"
     gene            complement(5778..5945)
                     /locus_tag="VKQ27_17825"
     CDS             complement(5778..5945)
                     /locus_tag="VKQ27_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90846.1"
                     /translation="MANAPIEYTTRQGDDGKWRIDTAQQKGTVIHHGVLDETFDTDEA
                     ASARISELKAE"
     gene            complement(5948..6235)
                     /locus_tag="VKQ27_17830"
     CDS             complement(5948..6235)
                     /locus_tag="VKQ27_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90847.1"
                     /translation="MIFGEYPCCNGHLCLSMPEKTPAYFPENCPHCGAKVWHRLSRVE
                     SMSWTEEDFLNEHDVDTAARTITAKPATEAEAFDMFSRQAGVTSAAALTEE"
     gene            complement(6232..6504)
                     /locus_tag="VKQ27_17835"
     CDS             complement(6232..6504)
                     /locus_tag="VKQ27_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90848.1"
                     /translation="MIERVARKRCEQEGWDPDATVLGEGEAAGRTWLGWQAYASQARE
                     LIAVMREPTDEMCDAGEMTSTGECDHMFAGKVFTAMIDEALEEDGR"
     gene            complement(6632..6919)
                     /locus_tag="VKQ27_17840"
     CDS             complement(6632..6919)
                     /locus_tag="VKQ27_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90849.1"
                     /translation="MADSMIERMAQALWSYEPWAQHETWDDLSMHGKAYWRERASLAV
                     RTMREPTEAMYRAGENSLSALDGVADFGQLEEHKLKLQWQAMVDAVLAEGN"
     gene            complement(6912..7268)
                     /locus_tag="VKQ27_17845"
     CDS             complement(6912..7268)
                     /locus_tag="VKQ27_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90850.1"
                     /translation="MPDEFGIVANVIETDHFLRTGAKVWVIHGWSGGGYERLVVRGLT
                     RGSSHAQKWVPIGRLSNFRAAWMPDKVRGKVWELQGTRDEMAARAAVMQSLAERMRVA
                     HPNRRFATLAQDAEHG"
     gene            complement(7268..7501)
                     /locus_tag="VKQ27_17850"
     CDS             complement(7268..7501)
                     /locus_tag="VKQ27_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90851.1"
                     /translation="MPDSTIERAVGYYWMRNLERPSVFVARWDGSHWTFIGSVFLIED
                     DQEDEYGERIMARYEVLDRIDEPARRTALAEKG"
     gene            complement(7494..>7745)
                     /locus_tag="VKQ27_17855"
     CDS             complement(7494..>7745)
                     /locus_tag="VKQ27_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90852.1"
                     /translation="ERARIAATSLRSQTVKYADLISNTSSIAARDPGFAGVYLEEKAA
                     TIALMGKDEWGLWRSAEASLQRGQEMLVQFALGGEAAHA"
BASE COUNT         1347 a         2585 c         2412 g         1401 t
ORIGIN      
        1 gccacacagg ccctgacgtc catcccaata tgacggccac ggcggcacag tgctccgctt
       61 ggtatcgcgc cgacatgacg gccaagatga aggctgtcct agcggtgtcg cccgagctcg
      121 ctggcaaccg caatgcactg atggcggccg gcgacctcgc ctacaactcc ggggctggca
      181 agtggcccgg ttcgccgatg cgggcggcct tcgcgaaggg ccagtgggcc gctggctgtc
      241 aggccttcgc cggctacatg accggataca aggctccgaa gcccgttccg gggcggaggt
      301 gctggatcag caagctcaac ggcaagctct attgcgagct accaggcctt gtcgtcaggc
      361 gtaagggcga gcgggatctc tgcctcggac tgccggcatg aggctcgtcg acctcgatcc
      421 gcgctggctc atgaaggacg gcgctcgcat cggcttcgtg tttcgatcac ccactctcgg
      481 ggacgatatg tggatgacgt gcttcgaagc gccgccgccg ctccgcgagc tatggcctat
      541 ggtgaaggac gcgttcccgg aaggaccggc gaacgagaag ggcttcactc gctcgatcca
      601 gccgatgaat caggcgcagc gctggcaaat cgccggcggg atagatgccg cgtcgttcga
      661 gacaatgacg gtcacgccat cgctcgacgg aagcccgggc ggcaactggc acggcttcat
      721 caccaacggc cagatcgtgg gtggtatctg atgggcgagt ggcaccaccc tgagcattac
      781 agcgagcagg caaagggctt cgcccgggcc atgcgcgtcg ctgagatcga cgtgttcgtc
      841 caggcggccc tgccgacgtt gatcggcgcc gcacggcatc atggttatgc gcttgcccga
      901 catggctcgg aagcgcgcga tctggacctt atcgccatac cgtggaccga aagcgcgagc
      961 gacgcggatt cgctgatcga ggcgctgtgc gaagcgacca ggcaggcaac cggctggggt
     1021 cattggtcgc accgtgggca tcgcggagag cgcgagcaaa agccccatgg ccgggtcgcc
     1081 gtgatgatcc ttgccacggc tgaagttcat atcgacctgt cggtcatgcc ccgcgtgccc
     1141 gctggccttg acgccgtcat ccctgaccac ctccgttgaa ggagacaacg atgaagatgc
     1201 ttactcgggc agccgcagtg ctgctcgcgc ttgttgcttg cgcgacgcca gccctcgccc
     1261 agcacgccac tttctcttac gcgccggcag gcccggcccc ggaagtcgct cccattgtgg
     1321 tggcggcgac gtccacgccg ctgacggcca cgcccgcagc cggcaccact gtctccgggc
     1381 cgttcacgcc acagttgggg cgcgacctgc ggatcatcat caaggggacg tgggcgggca
     1441 cgatttccgt cggaacgtcc gtcgacaact gcgcgaccgt caatccgctc acggcaggcg
     1501 gcgcgacgtg gggcagcttc accgccaatg ccaatgaggt ggtcgacacg ccgacgctcg
     1561 cgggcgtcgg ctattgtgtc acctcggtga ttacctcggg cactccgacg atcggactgc
     1621 gccagtgagc gcccgcctga tgcgcgcgct cacagcgctg ttgatcctcg ttgggttggg
     1681 ttccagcccc gcgtgggcat cgcccgaccc caccgcgcgc gcactcgcca tccaggcgag
     1741 cgctgctgcg accgctgctg tcgggccggc ctataccggc gccgccagcg tgcgcgagat
     1801 cggcaaccgc gttgccatcg cgaaccaaaa gtcgggatcg cagacgcggg ccggtttccg
     1861 cacttgggag gtcgattacg tcggcgggaa cccgaaggtc tgcttctcca atttcatcgt
     1921 caattcgacg gtaagcgggg tcgagaccgc gactggcggc gccatgaacc tcaagctcta
     1981 cgttgagctc aacggtgtcg tcacgccgtt cacatggggc ggcgcggcaa ccagctcgat
     2041 cgcgagtggc ggctatggct gcaccgatcc gtcgcctgtc gtcatcccgc cctacacgaa
     2101 ataccggctg tgcggggaca tgcagatggg ttcgggcgga acgatcccgt ccgagggctg
     2161 gtccaatagc gcggatctgc cgggcggaga tgaatatcag gtcggcacga cctcggactt
     2221 ctgtgccagc aacacgaagc tggcgacctc tgccgctgcg gcgatcatgc cggcgctcat
     2281 catcgcgccc agtagtcttt ccgtgtgggg cgtcgtcggc gacagcatca ccgccggggt
     2341 gaactatacc cagcagcaac cttccggtgg ccgcgggatc ctggcggcgc tggcggaata
     2401 tgggccggcg ctcaattacg gcgtccctgg cgacaaggcg acctggtacg cgacgaactc
     2461 aacggtccgg cgccagctac tcgcgctggg cggcgtcacg gcgctcgtcg acgagctcgg
     2521 cgtgaacgac ctcaccggct cccgcacggc ggcccagctg cttgctgatc gagcgacgat
     2581 ccgagggctc ttccctgggg tcacgatcta cgagacgacg atcatgccga cgacatcgac
     2641 cacggacggc tataagacgg ccgccaatca agctgtcgcc ggatcgagcg ctcagcgcac
     2701 atcgttcaac gataccgaac gtggcggggt cgcgggggtc gccagcctgc tcgacttcgc
     2761 ggctgaggtc gaaaccagca ccacgaccga gcaaggcttc gtcctcgatg ggggcgtctg
     2821 gacggcatca tgcatggtcg gatctgacgg cacgcatccg gttgagcaat gcgaacgtag
     2881 ccgccttgcg ccgctggtgc gcgcactgcg cagcaatcgg cgatgaggcg tctcagcctc
     2941 cacgcgcggg ccggcgcgat cgtcggcctg tccgtggcgc tgtggctcct catcggactg
     3001 gcgatcgcga ggttgctgtg agcggttggt agggagtcga acccacggcc gcctcggtat
     3061 cgggtatctg gccccacctc agcgaactag ccagtcgagc ccttactcgt tcgggttcag
     3121 ccttagacct ggctcggcaa caacgcgctc atagcatcga ctttgtaacc gccgcggccc
     3181 ggcgcgtcac tcccgggtgc catagctagg cagatccatc ggtcgccggc cgacttcttc
     3241 tccttcccgt cctgcaatct ccgggatgcc gcagcacgcg ggtcctatca tcaggagcgc
     3301 atcatgaaca tcaccccgct ggcgccccgc gccggcgccg cagccgcgcg ccttgtcgcg
     3361 tgggcgcagc aacgtgcgtc cgagcagtcg acgaagaccg gcttggtcgt gctcgctggc
     3421 gtgctcggtt ggcatctctc caccggtcag gccggcgaga tcgcgaacgc ccttcagatg
     3481 gtgctcggcc tgctcggcac cgcgctggcg gcggcaacca cctcttcacc ccatccgcca
     3541 gcgcagacag cgccggccga tccgcagccg gcggcgccgg tgcctctcgc ccacgaggac
     3601 caggccaata tcctgatccc cgacaatccc aacgtgctcc aggagaccaa tatgaccacg
     3661 atcctcgctg tgctcgaagc cgacgccatc gccatcctca aggcggccgg caaggcggtc
     3721 gagagcttct tcctcaccga aacccagaag ttcgtcgccc aggccaagca gacctatctg
     3781 ggcacgctcg gcctcaacct catccagacg atggaaagcc agtcgctgac cggcgagcag
     3841 aagatggaga ccgtcgtcgc gatgctcgtc ccggcgatcg agaagttcgt cgcgagcggc
     3901 ggcctgccca gcctcgtcgg ttcggtcgag gacttcgccc gcgagttcgc tcagtcgctc
     3961 ttcaacgact ttgcggccga cgtcgcgaag atcgctgcgc cgacgcaagc ggccgcgtga
     4021 acgcacgcgt ctgtcgcgtg attgctaccg cgccgcaggt caccgtcccg gaaacggggc
     4081 gccctgcggc gcaagtcctg acggacaacg cgtggcatgc cgagagcggg atctacctcg
     4141 agctcggcag cccgttgcct gatctgggca cggtgatcac ttgggaaggg cattgggttt
     4201 catggaccga tgcgaccggc caacaggtga aggcgcggaa ggtcgaatat gcttttgatc
     4261 cggacgccac gcttcactga tgtgcgagcg cgctcggtct gtgagtacgt cggtatttcg
     4321 tatcgatggg cgtcggattt aagacgtccc gggcgcaatt gctttttatc accgcacccg
     4381 gattaccggc caccattgat cgcgggggga tgtcggaggt aactacggcg ccgcccagga
     4441 tcctgctgcc ggcaccgatc gtcaccgcgc cgacgatgat agcatgcgga ccaatccaaa
     4501 catcatcttc gatcatggga catccgccga tagtgcggct ttctccgtcg agcctatcgg
     4561 ctcggccgag cgtggcgcca tgcatgatgg tgcagttggc accgatgcga gcatgttcgt
     4621 ttatgatgac accgaagccg tgggaaatgt ggaaggcagg accgatatca gttcgtagcg
     4681 gcatctccat gccggctcgc cgcgttgccc ggcgatgcca taaccacagg attggatagg
     4741 ccatttggcc gagcgcgctg ccacggagac gctggtatag gcgaatcggg accagtggcc
     4801 gaaaactacg gttgcgcgcg taaagtttca gaaatttcag gaaactatat tcaccgatca
     4861 cgcgatgggt atcgccagac agcattattg ctctccccac agtgaggcct tcaccgcaca
     4921 tatattggta cgtatccgtc acagtctatg gcgatgagcg ctatatttcg gtgcgctggc
     4981 taacagcacg actgcacggt cgtgatctgg gagacgggga agacgccggc aggctgctga
     5041 acgcgatcgc cgctgcgaag tggcggccga ttcccctcca cattgcgccc gccccgccat
     5101 atctttgcgg gatgcccgca atgaccttgc agaatatgcg cgagaacggc gccacctcgg
     5161 tcgaggcatg gtgtgcgtgc ggccatcacg ggccggtgga cgtcaccggc tggcccgatg
     5221 acatggaagt gccgaacgtg aagtggcgag tgcggtgttc gaagtgtggg cagcggccga
     5281 agcagaccgt gcccgcatgg ccggcgcgcc gcgggctgct cgattaggcg ggcggattaa
     5341 tatcggccca gtgcgtcacg tcggaaatcg gtgcgccctc atagagcgcc tcccagaacc
     5401 cgtccgtgcc ccactcttcc gacgggttcc atgttccgat gtccggcccc ggcgcgtcac
     5461 caccctgcca aagcaggatc tcccgcccgt ccttgcggtc gtccggaatg ttggcgatcg
     5521 gctgccagtc gatccctttg acgaagatca tagtcggcca ccccgtgagt gcgggccgta
     5581 gtagattggg cagggtcgca ctggccttgc gccataagcc acagcgtggg gcgtcggccg
     5641 cgttggttcc aattcgatcg gagttgaacg cttaggaaat agtgcggccc acagcttggc
     5701 gcgccccgcc tccagcgcga acagcacgcc cgcgccgaag acgatatgag cgatgatcgc
     5761 ggcttcatcg tgggacatca ctcggccttc agctcggaga tccgcgccga cgccgcctca
     5821 tcggtgtcga acgtctcatc gaggacgccg tggtggatga ctgtgccctt ctgctgggcg
     5881 gtgtcgatgc gccatttccc atcgtcaccc tggcgcgtcg tatattcgat cggtgcgttt
     5941 gccatcgtca ttcctcagtc agcgctgcgg cggaggtcac gcctgcctgc ctgctgaaca
     6001 tatcgaaggc ctcggcttcc gtcgccggct tggcggtgat cgtcctagcg gcggtgtcga
     6061 cgtcgtgttc gttgagaaag tcttcctctg tccacgacat gctctcaacg cggctgaggc
     6121 ggtgccagac tttcgccccg cagtgcgggc aattctccgg gaaataggct ggcgtcttct
     6181 ctggcatgga taggcagaga tgcccattgc agcaagggta ctcgccgaag atcatcgccc
     6241 atcctcttcc aatgcttcgt cgatcatggc cgtgaaaacc ttgccggcga acatgtgatc
     6301 gcactcgccg gtcgaggtca tctcccccgc gtcgcacatc tcgtcggtcg gctcacgcat
     6361 gacagcgatc aactcgcgcg cttggctggc ataggcctgc cacccgagcc acgtccgccc
     6421 ggcggcctca ccttcaccga gcacggtagc gtcgggatcc caaccttcct gttcgcagcg
     6481 cttgcgcgcg acgcgttcga tcattctgcc ggcgagcatc gacctatctc cgcccccgcc
     6541 aatcccgcca ggcggcacga aaaaatccgt tcaggaacgc gccgagagcc atcgatccga
     6601 gaatgatgag cacgagtttc agcgacatcg ctcagtttcc ctccgccagc actgcgtcga
     6661 ccatggcttg ccactgtagc ttgagcttgt gctcctccaa ctggccaaag tcagcgacgc
     6721 cgtcgagcgc acttaggcta ttctcgccgg cgcgatacat ggcctctgtc ggctcgcgca
     6781 tggtgcgaac agcgagtgac gccctttccc gccagtatgc tttgccgtgc atggaaaggt
     6841 catcccatgt ctcgtgctgc gcccacggct cataggacca aagggcctgc gccattcgct
     6901 cgatcatgct gtcagccatg ctcggcatcc tgtgcgagcg tggcaaatcg tcggttcgga
     6961 tgcgccaccc tcatgcgctc cgcgagagat tgcatcaccg ccgcccgcgc cgccatttcg
     7021 tccctcgtgc cctggagctc ccacaccttg ccccgcacct tgtcgggcat ccacgcggcg
     7081 cggaagttcg acagacggcc gatcggaacc cacttctgcg cgtgactcga tccgcgtgta
     7141 agaccgcgaa cgacaagccg ctcgtatccg cccccactcc agccatggat tacccagacc
     7201 ttcgcgccgg tcctgaggaa gtgatccgtc tcgatgacgt tcgcgacgat gccgaactcg
     7261 tcgggcatca ccccttctcc gccaaggccg tgcgtctcgc aggctcatcg atacgatcaa
     7321 gcacctcgta tcgagccatg atgcgttccc cgtactcatc ttcttggtca tcctcgatca
     7381 ggaagaccga gccgatgaac gtccagtgcg agccgtccca gcgcgcgacg aaaacgctag
     7441 ggcgctccag attgcgcatc cagtagtagc cgaccgcccg ctctatcgtg ctgtcaggca
     7501 tgggccgctt ctccgccgag ggcgaactgc acgagcatct cctgcccccg ctgaagcgat
     7561 gcctcggccg acctccaaag gccccactca tcctttccca taagggcgat ggtcgcagcc
     7621 ttctcctcca gatagacccc cgcgaaaccg gggtcgcgcg ccgcgatcga cgacgtgttc
     7681 gagatgaggt ccgcgtattt gacggtctgg gaccgcagag acgtagcagc gatgcgggcc
     7741 cgctc
//
LOCUS       DATGEM010000584         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_837313, whole genome shotgun sequence.
ACCESSION   DATGEM010000584 DATGEM010000000
VERSION     DATGEM010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837313"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VKQ27_17860"
     CDS             <1..287
                     /locus_tag="VKQ27_17860"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90853.1"
                     /translation="ATLTMHHGREAAEARIADPTPIPSEYVARTRRNMTHAQHQVQTA
                     VEKLVEVCGARSVYDADPLATLRRDLLTVLTHNVASRQAAYAAWGKQALT"
     gene            complement(300..1028)
                     /locus_tag="VKQ27_17865"
     CDS             complement(300..1028)
                     /locus_tag="VKQ27_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90854.1"
                     /translation="MSQSVVAELAPHGVLRAGINMSNFLLVTGRSPSGDPDGVSPDMA
                     RAIADKLGVPVKYVPFPKPGELADAAGTDTWDIGLIGAEPARAEKIAFTAAYVEIEAT
                     YLVPAGSPIQRIADVDKPGIRIAVTARSAYDLWLERNIKQATLVRSDSLASATEKFAN
                     DKLDALAGLRPGLLGDIQKFPGARILDGQFTAVQQAVGTARVNTAGAAFLRDFVEEAK
                     RSGLVAKLIEKHGVVGRLSVAPPG"
     gene            complement(1032..1385)
                     /locus_tag="VKQ27_17870"
     CDS             complement(1032..1385)
                     /locus_tag="VKQ27_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLY90855.1"
                     /translation="MSGLFARVFGIKSAALTWFDVADVQSRLGDVLLVDVREPQEYHS
                     APGHLPGALNVPLAEVQGRADELGASGKPVVLVCKTDRRSARAAEVLLAAGLRDVAVL
                     RGGTDGWHAQGLELE"
     gene            complement(1393..>2451)
                     /locus_tag="VKQ27_17875"
     CDS             complement(1393..>2451)
                     /locus_tag="VKQ27_17875"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HLY90856.1"
                     /translation="HRIAVVPGDGIGKETVPEALKVLDAASRRFGFKLEYAHYDWSCE
                     TYKKTGAMMPADGMEQLAQSDSILLGAVGWPDVPDHISLWGLLIPIRRGFDQYVNLRP
                     CKLMPGVRTPLAGRTEADIDFYVVRENTEGEYSSVGGRMFVDTDREFVSQQSVFSRHG
                     VDRILTFAFDLAMTRPKKHLTSATKSNGITFTMPYWDERFALMGKSYPEIRTDQFHID
                     ILCAHFVQHPDWFDVVVGSNLFGDILSDLGPAVAGSIGIAPSANINPERKFPSMFEPV
                     HGSAPDIAGKFICNPIGQVWSAGLMLEHLGEVDAGRAIVAAIETLLRESGPKTRDMGG
                     QAGTADVGKALAEIVAGG"
BASE COUNT          492 a          852 c          703 g          404 t
ORIGIN      
        1 cggcaacact cacgatgcac cacggccgcg aagccgccga agcgcgcatc gccgacccaa
       61 cgcccatccc atcggaatac gtggcccgca cccgccgcaa tatgactcac gcgcagcacc
      121 aggtgcagac ggccgtggag aaactggtcg aagtctgcgg cgcgcgctcg gtctacgacg
      181 ctgacccact ggccacgctg cgccgcgact tgctgaccgt cctcacccac aacgtcgcca
      241 gccgccaggc tgcatacgcc gcctggggca agcaggccct cacctgatcg ctgtttcgat
      301 caccccggcg gtgcgacaga caaccggccc acaaccccat gcttctcgat cagcttcgca
      361 accaggccgg accgcttcgc ctcctcgacg aagtcccgca ggaaggccgc gcccgctgta
      421 ttgacgcgcg ccgtgccaac ggcctgctgt accgcggtga actggccatc aaggatccgc
      481 gcgccaggaa acttctggat gtcgccaagc aggccaggac gcaacccagc cagcgcatcc
      541 agcttatcgt tggcaaactt ctccgtcgcg ctcgccagac tgtctgagcg caccagtgta
      601 gcctgcttga tgttgcgctc cagccagagg tcataggcgc tccgtgccgt gaccgcgata
      661 cggataccgg gcttgtccac atcggcaatg cgctggatcg gcgagccggc cggcaccaga
      721 taggtcgcct cgatctcgac ataggccgcg gtgaacgcga tcttctccgc gcgtgcaggt
      781 tcggcgccga tcagcccgat atcccatgta tcggtaccgg ccgcatcggc aagctcgccc
      841 ggcttgggaa acggcacata tttcaccggc acgcccagtt tatcggcgat ggcgcgcgcc
      901 atatcgggcg atacgccatc cggatcgccg gacggcgagc gccccgtgac gagcaggaag
      961 ttgctcatgt tgattccggc gcgcagcacg ccatgcggag ccagttcagc gacgacggat
     1021 tgggacattg ttcactccaa ttccagacct tgagcgtgcc agccatccgt accgccacgc
     1081 aacaccgcga cgtctcgcag gccggcggca agcagcacct cagcagcacg agcagaacgg
     1141 cgatcggtct tgcagaccag cacaacgggt ttgccgctgg caccgagttc atcagcgcgg
     1201 ccttgcactt ccgcaagcgg cacattcagc gcgccaggaa ggtggccggg agccgaatgg
     1261 tactcctgcg gctcgcgcac atcgaccagc agcacatcgc caagccgcga ctgcacatcc
     1321 gcaacgtcga accacgtcag agccgcgctc ttgatgccga acacgcgggc gaacaatcca
     1381 ctcatcctaa ggtcagcctc ccgccacgat ctccgccaga gccttaccga catcggcggt
     1441 acccgcctgc ccgcccatat cgcgggtctt cggaccactc tcgcgcagca gcgtctcgat
     1501 cgcggcgaca atggcacggc cggcgtccac ctcgcccaga tgctccagca taagtccagc
     1561 cgaccacacc tgcccgatcg gattgcagat gaacttgccg gcaatgtcgg gcgcggaacc
     1621 gtgtaccggc tcgaacatcg agggaaactt ccgttccgga ttgatattgg cggaaggtgc
     1681 gatgccaatg gatcccgcaa ccgccggccc gagatcactc aggatatcac cgaacagatt
     1741 tgacccgacc acgacatcga accaatccgg atgctgcacg aaatgcgcgc aaagtatgtc
     1801 gatgtggaac tggtcggtac ggatttccgg atagctcttg cccatcagcg cgaaacgctc
     1861 gtcccagtac ggcatggtaa acgtgatgcc gttcgacttg gtggccgacg tcaggtgctt
     1921 cttcggccgc gtcattgcga gatcgaatgc gaatgtcagg atgcgatcaa cgccgtggcg
     1981 gctgaacacg ctctgctggc tgacgaactc gcggtcggta tcgacgaaca tccggccgcc
     2041 aaccgacgag tactcgccct cggtattttc acgaactaca tagaaatcga tatcggcctc
     2101 ggtgcgaccg gcaagcggcg tccgcacgcc aggcatgagc ttgcagggac gcaggtttac
     2161 atattggtcg aagccacggc ggatcgggat cagcagtccc cacagcgaaa tatggtccgg
     2221 cacgtccggc cagccaacgg cacccagcag aatcgaatcg gattgcgcca gctgctccat
     2281 cccatcggcc ggcatcatcg cgccagtctt cttgtaggtc tcacaggacc agtcgtagtg
     2341 ggcatattcc agcttgaagc cgaatcggcg cgacgcggcg tccagcacct tcagcgcttc
     2401 cggcaccgtc tccttgccga ttccatcgcc gggcaccacc gcgatcctgt g
//
LOCUS       DATGEM010000585         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1246600, whole genome shotgun sequence.
ACCESSION   DATGEM010000585 DATGEM010000000
VERSION     DATGEM010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246600"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..912)
                     /locus_tag="VKQ27_17880"
     CDS             complement(370..912)
                     /locus_tag="VKQ27_17880"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HLY90857.1"
                     /translation="MRYFTILGSVIAFAALPAAAQTNPPALAYIEPLPPAAVQNVQAK
                     LQQVGGYTGRIDGIWGTDSEAALERFQQQHQLQVTGQLNEATAATLGLNPTTLLATAA
                     PAPAPPPADHLLPASVRAVQARLGTLGFYNGPVDGVWGQGTEMAIQGFQRARSLPADG
                     QLGPATVTAMGLGPDVLAYR"
     gene            complement(1136..1801)
                     /locus_tag="VKQ27_17885"
     CDS             complement(1136..1801)
                     /locus_tag="VKQ27_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HLY90858.1"
                     /translation="MCGRYASFLPAEALRTLFHTENPTPNWEPTWNMAPTRDAPVVRL
                     HPETRARHLDLLRWGLVPHWAKDPKTVRQPINARAETLATSPMFRDALARRRCLVPAD
                     AFYEWQKTDAGKQPYAIARADGQPMVFAGLWEGWRGTDGSVMRTFTIVTTNANTILRP
                     LHERMPVVLEPVDWPTWLGEIEGDPAGLLRPSSADLRVWRVGNAVNNVRNDSAALLAE
                     VVV"
     gene            complement(1801..3342)
                     /locus_tag="VKQ27_17890"
     CDS             complement(1801..3342)
                     /locus_tag="VKQ27_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLY90859.1"
                     /translation="MSPAQNDQAPYRLIITLCTVGATLMQALDQTIANVALPYMQGSL
                     SASFDEITWVLTSYITAAAIMTAPVGWLASRFGRKYLFVTCLIGFTITSMLCGAAQSL
                     DEMVTFRLFQGMFGAALVPLSQATMLDIYPAEQRGNAMAIWGVGVMIGPILGPTLGGY
                     LTEMYNWRYVFYINLPFGILATLGLIFFMPRSTPNTSMRFDWTGFAALSLGIGAIQMM
                     LDRGQDQDWFTSREIIIEAVLGGLGVYLFIVHMLTARRPFIPPALFKDRNFAAGVVMM
                     FAAGTILVSSSSLMAPWLQNLANYPVETAGLIMAPRGIGTMVAMVLAGRIATRVDPRM
                     AMGFGILCVVWSIWEMTSWTPDINEGRLAFTIVVQGFGLGFLFIPLQMLAFATLPVML
                     RTDGAGLFSLFRNIGAAIGVSVTSSMLARNTQVLHAEIGSHVTPFNRALQDGGAVQHH
                     MAPATHHGMMMLDRLVNQQAQIIAYMDDYKMMIFTTLPALLLLFLMRRPRQVVQTADS
                     HAAMD"
     gene            complement(3472..>3791)
                     /locus_tag="VKQ27_17895"
     CDS             complement(3472..>3791)
                     /locus_tag="VKQ27_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLY90860.1"
                     /translation="DVWIEANPKETELTYVKDGDPVDVVVDTYPSRTWKAVVESIAPN
                     SGSEFSVLPAQNTSGNWVKVVQRIPVRIHVERKAGDPELRAGMSVEAEIDTGHHRTLA
                     DLF"
BASE COUNT          670 a         1171 c         1280 g          670 t
ORIGIN      
        1 ccgatttatc cgtttctcct ggatctcttt tggctccgct gtgaaaagag cgctggatgt
       61 gcattggcga gcgcggtggg cccgatcgcc gtagggatcg gtgatggctg gccgaatcac
      121 gtgtcggtcg gcatgggcgt agccgcactg gtgctatccg gtgtcggttg gctgtttggg
      181 ctttggctga cacgccaccc gttcctcggg gaaatccgtc gcgcagccgt tgcaatacga
      241 gggcatccaa ttgcgaccaa gctcttgcgc cagccgacaa caatcagctg tagcggtacc
      301 gacggggcct gattgtgcgg atggtagcgt gtatcggggc cgtcctcgat gggcgagccg
      361 cgcgctgggc tatcgatacg ccaggacgtc tggccccagc cccatcgccg ttaccgtcgc
      421 aggtcccagc tgcccatcgg ccggcaagct gcgcgcccgt tggaagcctt gtatcgccat
      481 ctctgttcct tgcccccata cgccgtccac agggccgtta tagaagccga gtgtccccag
      541 tcgcgcctgg acggcgcgga cggatgccgg gagcaggtgg tccgcgggag gcggagcagg
      601 agccggtgcg gctgtggcga ggagcgtggt cgggttcagg ccgagggttg cggcggtcgc
      661 ctcgttcagt tgcccagtca cttgcaattg gtgctgctgc tggaagcgct ccagcgctgc
      721 ctcgctgtcg gtaccccaga tgccgtcgat gcgcccggtg tagccgccca cttgctgcag
      781 ttttgcctgc acgttttgca ccgcggcggg gggtaacggc tcgatgtaag cgagggccgg
      841 cgggttcgtt tgcgcggctg ccggcagggc cgcaaaggcg atgacgctgc cgaggatcgt
      901 gaagtaccgc atggacgtgc tcctctggac gtttgcagag aagaacgtcg gtcaagccca
      961 acggttctcc ggacgcctgc ggtgagggcg ccaacaatcg ctcgatcggg tcacgcagtg
     1021 gagccggcgg acgtgcagcc agcgccttcg ctttcgcgtg atcgggccag cggcagatct
     1081 gctgcgcgaa gatcgagtta tttcgccgtt cagcctgacc gctcaggcct tccgatcaca
     1141 ccacaacctc cgccagcagc gccgcgctgt cgttgcgcac gttgttcact gcattgccga
     1201 cccgccagac ccgcaggtcg gcggacgaag gccgaagcag gccggcaggg tcaccttcga
     1261 tttcgccgag ccaggtcggc cagtccaccg gttccagtac aaccggcatc cgctcatgca
     1321 gcggacggag tattgtattg gcgtttgtag tgacgatcgt gaatgtgcgc attaccgagc
     1381 cgtctgttcc ccgccagccc tcccataagc ccgcgaacac catcggctgg ccatcggcac
     1441 gcgcgatggc gtacggctgc ttgcccgcat ctgtcttctg ccactcatag aacgcatccg
     1501 ccggcaccag gcagcgccgg cgcgccagcg cgtcgcggaa catcggcgaa gtagccagcg
     1561 tttcagcgcg cgcattgatt ggctgacgca cagtcttcgg atccttcgcc caatgcggca
     1621 ccaggcccca gcgcagcaga tcgaggtgtc gcgcgcgggt ctccggatgc agccgcacca
     1681 ccggcgcgtc gcgcgtcggc gccatgttcc aggtgggctc ccagttcggt gtcgggttct
     1741 cggtgtggaa cagagtgcgc agcgcctcag cggggaggaa cgaggcatag cggccgcaca
     1801 tcagtccatt gccgcatgcg agtcggcggt ctgcacgacc tgacgggggc ggcgcatcag
     1861 gaacagcagc agcagcgccg gaagcgtggt gaagatcatc atcttgtagt catccatgta
     1921 ggcgatgatc tgcgcctgct ggttgaccag gcggtccagc atcatcatgc cgtgatgcgt
     1981 cgcgggcgcc atatggtgtt gcaccgcgcc gccgtcctgg agcgcacggt tgaacggagt
     2041 gacatgggag ccgatctcgg cgtgcagcac ctgcgtgttg cgtgccagca tcgacgacgt
     2101 cacggatacg ccgatggccg cgccgatatt gcggaacagg ctgaacagac ccgcgccgtc
     2161 ggtgcgcagc atcaccggca gcgtcgcaaa ggcgagcatc tgcagcggta tgaacagaaa
     2221 gccgaggccg aacccctgca cgacgatggt gaaagccagg cggccctcgt tgatgtcggg
     2281 cgtccagctt gtcatttccc agatcgacca cacgacgcac aggatgccga agcccatggc
     2341 catgcgggga tcgacccggg tcgcaatgcg gccggccagc accatggcga ccatcgtgcc
     2401 gataccacgc ggggccatga tcaggccggc ggtctccacc gggtagttgg cgaggttctg
     2461 cagccagggc gccatcagcg acgaactgga aacgaggatc gtgccggcgg cgaacatcat
     2521 caccacgccg gccgcgaaat tgcggtcttt gaacagcgcg ggtggaatga acggacgtcg
     2581 cgctgtcagc atgtggacga tgaagagata gacgcccagg ccgccgagga ccgcctcgat
     2641 tatgatctcg cgtgaggtga accagtcctg gtcctggccg cggtcgagca tcatctggat
     2701 agcgccgatg ccgaggctga gcgcggcgaa gccggtccag tcgaagcgca tggaggtatt
     2761 cggcgtggaa cgcggcatga agaagatcag gccgagcgtc gccaggatgc cgaacggcag
     2821 gttgatgtag aagacgtacc gccagttgta catctcggtg agatagccgc cgagcgtggg
     2881 acccaggatc gggccgatca tgacgccgac tccccagatc gccatggcat tgccgcgctg
     2941 ctcggcagga tagatgtcca gcatggtggc ctgcgagagc ggcacgagtg ccgcgccgaa
     3001 catcccctgg aagaggcgga acgtcaccat ctcgtccagc gactgggcgg caccgcacag
     3061 catggaggtg atggtgaagc cgatcaggca ggtgacgaac aggtatttgc ggccgaaccg
     3121 gctggccagc cagcccaccg gcgcggtcat gatggcggcg gcggtgatgt aggaggtcag
     3181 gacccaggtg atctcgtcga agctcgccga gaggctgccc tgcatatagg gcagcgcgac
     3241 gttggcgatg gtctggtcca gcgcctgcat cagcgtcgcg ccgacagtgc agagggtgat
     3301 gatcaggcga tagggcgcct gatcattctg agcaggtgac atggcgaatt tagcagccgg
     3361 ttgtttcgcg tgtcatcgcg agcaaagtga agcaatcccg gggcaatctg gcgcacgcgt
     3421 tcaagccgag attgccgcgc cggcttcgcc tcctcgcaaa gaccgtagac atcagaacag
     3481 gtcggccagc gtgcggtgat ggccggtgtc gatctcggct tcaacgctca tgcccgcgcg
     3541 gagttcggga tcgcccgcct tgcgctcgac atggatgcgg acagggatgc gctgcaccac
     3601 cttcacccag ttgcccgagg tgttctgcgc cggcaggacc gagaactccg agccggagtt
     3661 gggggcgatg ctctccacca cggccttcca ggtgcggctt gggtaggtgt ccaccacaac
     3721 atcgaccgga tcaccgtcct tcacgtaggt gagctcggtt tccttcggat tggcttcgat
     3781 ccagacatct t
//
LOCUS       DATGEM010000586         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_506379, whole genome shotgun sequence.
ACCESSION   DATGEM010000586 DATGEM010000000
VERSION     DATGEM010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506379"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VKQ27_17900"
     CDS             complement(<1..326)
                     /locus_tag="VKQ27_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY90861.1"
                     /translation="MPDFQNILVETHGRVGLIRLNRPQALNALCDELMEELGTQLLAF
                     DADRAIGAIVLTGSEKAFAAGADIKQMKDRVYPDVLLDDFIARWETILRVKKPVIAAV
                     AGFALGG"
     gene            complement(339..1178)
                     /gene="mutM"
                     /locus_tag="VKQ27_17905"
     CDS             complement(339..1178)
                     /gene="mutM"
                     /locus_tag="VKQ27_17905"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HLY90862.1"
                     /translation="MPELPEVETVMRGLRERLEGRTIVRAEAHRPDLRWPLPEGLQHR
                     LTGARVTGFRRRGKYILMRLNGGDSVLLHLGMSGRMILSPVRPNLPTPHEHLVLETND
                     GWRLGFVDPRRFGSVDLVPTKQEDSHRLLAELGPEPLDPAFTPARLSTSLIGKRTPIK
                     AALLDQKIVAGLGNIYVCEALFRSRLSPQRSAYTIPGTRAARLVPAIKNTLNEAIAAG
                     GSSLRDYVQPDGELGYFQHAWKVYGREGEPCDRCPGRPACPGIRRITQSARSTFYCPR
                     SQR"
     gene            1208..1963
                     /locus_tag="VKQ27_17910"
     CDS             1208..1963
                     /locus_tag="VKQ27_17910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90863.1"
                     /translation="MSDNPASADTTDFGYQRVPRAAKRGMVRAVFDSVAPRYDLMNDL
                     MSLGVHRVWKDIFVTALAPNPRHGLLDLAGGTGDISFGWLEHGGGPVLLSDVNAAMLE
                     VAQDRAVSGAMIADVSLLVADAERLPLPDRTVDRVSIAFGLRNCTDKAAVLREARRVL
                     KPGGRFFCLEFSHVQVAAMQPLYDAWSFRVLPMLGRYVAKDEASYRYLAESIRTFPDQ
                     EMLAAMLREAGFARVSVRNLSGGIAAIHSGWRL"
     gene            1960..3159
                     /gene="coaBC"
                     /locus_tag="VKQ27_17915"
     CDS             1960..3159
                     /gene="coaBC"
                     /locus_tag="VKQ27_17915"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLY90864.1"
                     /translation="MISGKRVLLIISGGIAAYKALELIRLLRGRGCAVTCALTDNGAQ
                     FVTPLSLQALSESKVYAELFSLTDESEMGHIELSRSADLVVVAPATANILAKMAGGLA
                     DDLASTLLLATDKPVLVAPAMNVRMWEHAATVANMALLASRGIHVVGPDEGPMACNEY
                     GFGRLAEPPAILAAIEGLLSPARPLAGKHVLVTSGPTHEPIDPVRYIANRSSGRQGHS
                     IAAAVAALGARVTLVTGPVVVPDPAGVTVKRVESAQQMLDACQNALPVDAAVFAAAVA
                     DWRVASTSKGKIKKVPGEAAPALELVPNPDILATIAAVGPRRPRLVVGFAAETDDLMQ
                     NAQAKRVRKNADWIVANDVSPATGIMGGEENAVHLITGDGVEDWPRLAKQEVARRLAE
                     RIAQTLR"
BASE COUNT          513 a          949 c         1153 g          592 t
ORIGIN      
        1 ccgccgagcg cgaaaccggc gaccgcggcg atcaccggct tcttcacacg cagaatggtt
       61 tcccagcgcg cgatgaagtc gtccagcaac acgtcgggat acacgcgatc cttcatctgc
      121 ttgatgtcgg cgccggcggc gaacgccttc tcggagcccg tcagcacgat cgcaccgatc
      181 gcgcgatccg cgtcgaaagc caacagttgc gtgccgagtt cctccatcag ttcgtcgcac
      241 agcgcgttca gagcctgcgg ccggttgagg cggatcaggc cgacgcggcc atgcgtttcc
      301 acgaggatgt tctgaaaatc gggcatctgt cgcttctcct atcgctggct tcgcggacaa
      361 tagaacgtgc tgcgcgccga ctgcgtgatg cggcggatgc cggggcaggc gggacgcccg
      421 gggcaccggt cgcacggttc gccctcgcgg ccatagacct tccaggcgtg ctgaaaatac
      481 ccgagttcgc catccggctg cacatagtca cgcagcgatg atccgccagc ggcgattgct
      541 tcgttcagtg tgttcttgat cgcgggcacc agccgtgcgg cgcgtgtgcc cgggatcgta
      601 tatgctgatc gctgcggact gagacgcgag cgaaacaacg cctcgcacac gtaaatgttg
      661 ccaaggccgg cgacaatttt ttggtcaagc agcgccgcct tgatcggggt gcgcttgccg
      721 atgagcgatg ttgagaggcg ggcaggggtg aatgccggat ccagcggctc tggccccagc
      781 tcggccagca gcctgtggga gtcctcttgt ttcgtgggca cgaggtccac ggagccgaag
      841 cggcggggat cgacgaagcc caggcgccag ccgtcgttgg tttccagcac cagatgctcg
      901 tgcggcgtcg gcaggttagg ccgcaccggc gacaggatca tgcggccgga catgcccagg
      961 tgcagcagca cggaatcgcc gccgttcagg cgcatcagga tgtatttgcc gcggcggcgg
     1021 aaaccggtga cgcgcgcgcc ggtaaggcgg tgctgcaggc cttctggcag cggccagcgc
     1081 aggtccggac ggtgggcttc cgcgcgcacg atggtgcggc cctccaggcg ctcgcggagg
     1141 cctcgcatta cggtctcaac ttcggggagt tccggcatgg gggcagggca cgctatagat
     1201 ggcgcagatg agcgacaacc ccgcctcggc cgacaccacc gatttcggct accaacgtgt
     1261 cccgcgcgcc gcaaagcgcg gcatggtgcg tgccgtattc gacagcgtcg cgccacgcta
     1321 cgacctgatg aacgacctga tgtcgctcgg cgtgcatcgg gtgtggaagg atatctttgt
     1381 cacggcgctt gctcccaatc cgcggcacgg gctgctggat ctggcgggtg gcaccgggga
     1441 tatctcgttc ggctggctcg agcatggtgg cgggccggtg ctgctgagcg atgtcaacgc
     1501 agcgatgctg gaagtggcgc aggatcgtgc ggtgtcgggc gcgatgattg ccgacgtgtc
     1561 gctgctggtg gccgatgccg agcgcctgcc gctgcctgat cgcacggtgg atcgcgtgtc
     1621 gatcgctttc ggtttgcgca actgcaccga taaggccgcg gtgctgcgcg aggcgcgccg
     1681 ggtgctgaag cccggcgggc ggttcttttg cctggagttt tcccacgtgc aggtcgccgc
     1741 gatgcagccg ctgtatgatg cgtggtcttt ccgcgtgttg ccgatgctgg gacgctacgt
     1801 cgcgaaggat gaggccagct atcgctatct ggctgagagc atccggacat tccccgatca
     1861 ggagatgctc gccgcgatgc tgcgcgaggc gggcttcgcg cgtgtttcgg tccgtaattt
     1921 atcgggcggc atcgccgcca ttcattcggg ctggcggcta tgatctctgg caagcgggtc
     1981 ttgctgatca tctcgggcgg cattgccgcg tacaaggcac tggagctgat ccgtctgctg
     2041 cgcggccggg gttgcgcggt gacatgcgcg ctgaccgata atggcgcaca attcgtcacg
     2101 ccgctgtcgt tgcaggcgtt gagcgagagc aaggtctacg ctgagctgtt ctcgcttacc
     2161 gatgagagcg agatggggca catcgagctt tcgcgcagtg ccgatctggt ggtggtggcg
     2221 ccggccacgg cgaatattct ggcgaagatg gcgggcgggc tggcggacga tcttgcgtcc
     2281 accctgttgc tggcgaccga caagccggtg ctggttgcgc cggcaatgaa tgtgcgcatg
     2341 tgggagcacg cggcgactgt ggccaatatg gcgctgcttg cctcgcgcgg catccatgtt
     2401 gttgggccgg atgaaggtcc gatggcctgt aacgagtacg gctttgggcg gctggcggaa
     2461 ccgccggcga tcctcgcggc gatcgaggga ctgctgtcgc ctgcacggcc gctcgctggc
     2521 aagcatgtgc tggtgaccag cgggccgaca cacgagccaa ttgatccggt acgctatatc
     2581 gccaatcgat ccagcggtcg gcagggccat tcgatcgctg ctgcggttgc ggcgttgggc
     2641 gcgcgcgtga cgctggtcac cgggccagtg gtggtgccgg atccggcagg cgtcacggtg
     2701 aagcgagtgg aaagcgcgca gcagatgctg gatgcgtgcc agaatgcgct gccggtggat
     2761 gcggcggttt ttgccgcggc ggtggcggac tggcgggttg ccagcacatc gaaggggaag
     2821 atcaagaagg tgccgggcga ggcggcgcct gcactggaac tggtgccaaa tcccgacatt
     2881 ctggcaacga tcgcggctgt gggtccgcgg cggccgcgcc tggttgttgg tttcgcagca
     2941 gaaacagacg atctgatgca aaatgcgcag gccaagcggg tgcgcaagaa tgcggactgg
     3001 attgttgcga acgatgtttc gccggcaacc ggcattatgg gcggcgagga gaatgccgtg
     3061 cacctgatta ccggcgacgg cgtggaggac tggccgcggc ttgccaagca ggaggtggca
     3121 cggcggctcg ccgagcggat cgcgcagacg ttgcgctaag cggctttctt ctgccggggc
     3181 cagcgcgtgg cgaccatggg cagcacc
//
LOCUS       DATGEM010000587        12221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_880479, whole genome shotgun sequence.
ACCESSION   DATGEM010000587 DATGEM010000000
VERSION     DATGEM010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12221
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880479"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1461
                     /locus_tag="VKQ27_17920"
     CDS             247..1461
                     /locus_tag="VKQ27_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLY90865.1"
                     /translation="MPAAPPTLAANVMHFARLLRRAGLPVGPAETLAGQQALSLIDLG
                     DRIQARTALRAAMVHRHEHQDVFDHAFNLFWRDPTAAEQAAAMALLDAQKDKRPERPP
                     PGSRRVAEALARPRETKPRDDEPPVMDAVLTVSDRERLQQMDFEAMSAAEIAEAKREI
                     QRLVLPLDLRRTRRLRADPLGPVTDLRRTIRQSLRQGGEILTIARERHVTRPPPLVVL
                     CDISGSMSRYAQILLHFLHAVTNDRDRVHVFLFGTRLSNVTRQLRYRDPEVAFQMVSQ
                     IVPDWSGGTRIGEALESFNRFWARRVLGQGAVVLLITDGLDRDGARGLADNMDRLHRS
                     CRRLIWLNPLLRWSGFEPKSQGIRAMLPHVDDFRPVHNLASLRALVDLLSRPTPQRTP
                     LAPAREPERMAS"
     gene            1458..1784
                     /locus_tag="VKQ27_17925"
     CDS             1458..1784
                     /locus_tag="VKQ27_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLY90866.1"
                     /translation="MNIEEFEDVLSTAAGWRAAGEQVALATVTETWGSSPRPAGSQMA
                     VTKSGKMAGSVSGGCIEGAVADAAMKTIDTGTPQLLDFGVTHERAWEVGLACGGKVKV
                     FVEKLS"
     gene            1781..2485
                     /locus_tag="VKQ27_17930"
     CDS             1781..2485
                     /locus_tag="VKQ27_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLY90867.1"
                     /translation="MTPELLSALEAAKREKRPVVLATRLPDGDQCLLPDPSAAAALNA
                     EAEKVLQSDESGTVKIDGTDWFLHAHNPPLRLVVVGAVHIAQALVPFATQCGFAVVVV
                     DPRRSFASDERFPNVTVSTAWPDEALDELKPDARTAVVTLTHDPKLDDPALDRALKSR
                     AFYIGSLGSRRTHAARLKRLRELGHGDNALQRIQGPVGLNIEAVTAPEIALSIIAELV
                     AVRRGAPLPHRQPLAA"
     gene            2482..4140
                     /locus_tag="VKQ27_17935"
     CDS             2482..4140
                     /locus_tag="VKQ27_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HLY90868.1"
                     /translation="MKFGPVPLDEARGAIMAHSQRVGERMIRKGAVLDEPALLALREA
                     GRDEVIVARLEPGDVPEDIAADRLAEPLISPLLARTRAATGRVNLTAEAPGLLRVNTA
                     MINRLNTIDESITLGTLPDYAVVAPRDLVATVKIIPFAVSRTVLTVAEALVRQAGSPL
                     TLHPFRHLKVGLVVTELPGLKDSVTEKTIAATEQRVTQLTGTLLPPLHTPHEEVPIGR
                     ALERLIEQGADALLVVGASAVVDKRDVGPMGIVQAGGEILHFGMPVDPGNLICLGRIG
                     TRPALVLPGCARSPKLNGIDFVLSRLFADLPMGPGEIMRMGVGGLLKEIDIRPLPREK
                     APATPRVGAAPRSAPTIAVIVLAAGRSRRMAPHNKLLVVDRTGKPMIARVVDNVLSSH
                     ARPILVVTGHQAEQVQHALGGRPVRYVHAEDYAEGLSASLKAGIAAVPEECAAAVVCL
                     GDMPLVTGRMIDRLLSAYDPDEGRLIVLPTYHGKQGNPMLWDREFFPEIMDVSGDSGA
                     RFLVGQHIEAVAEVEMADDAILRDFDTTESLSTLPQRLRPEGVG"
     gene            4142..5044
                     /locus_tag="VKQ27_17940"
     CDS             4142..5044
                     /locus_tag="VKQ27_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90869.1"
                     /translation="MPICEADPWRFQYFEKVACPEHVRISTEDPDSWEWFPEHRWIYD
                     KLAVALSQGLQAAPHGVMPPSFPIFSKPITNLRGMGTGSQVIHSADEYRTALTAGHFW
                     CTLLTGAHVSTDVALVDGEPRWWRHTTGVTATGGTFDYWLVQAAPIPEIEDWCGAWSR
                     QHLRGYTGMANFETIGGRIIEAHLRFADQWPDLYGKGWVEAVVDLHATGDWTYPDGDR
                     TDGYSVVLFGPHGPRYRHPPDRLVQEVLAMPGVSSVQITFHEDKAPEHHAMPPGGFRL
                     AIVNAVSLDAGRVGRAKLGAALLG"
     gene            complement(5059..6327)
                     /locus_tag="VKQ27_17945"
     CDS             complement(5059..6327)
                     /locus_tag="VKQ27_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY90870.1"
                     /translation="MGDPARLTATEAAQLIREKKLRPEALMEACLARIAEREASVRAF
                     AWFDAAAALNAAGGARPGPLHGLPVGVKDVLDTADMPSEYGSPIWSGWRPRADAAAVA
                     WARAAGGVVIGKTVTTEFATRKPGPTGNPHNLAHTPGGSSSGSAAGVADYFFPLAYGT
                     QTAGSVIRPAAYCGVVGFKPTFATINRFGMKLMAESLDTVGVMARSVADCALFAGAVA
                     GRNLGNPEVRPEHAPRIGMCRSPAWDKAAPETQHLLERVASALARAGASVVDRELSPE
                     VAAVAEAHTIVMNNESGRAMGWELVHAPELISDGLRERLEFGLSRSETEVLEAHATFE
                     RAQYALPGCMEGLDVLVTPSAPGEAPAGLEWTGDPVFNLIWTSLHVPCVTVPAGTGPN
                     GLPLGIQIVARIGEDRQALAWAQWVSAAIG"
     gene            6392..6940
                     /locus_tag="VKQ27_17950"
     CDS             6392..6940
                     /locus_tag="VKQ27_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90871.1"
                     /translation="MMQPSEHPASVHVGTGPDGSLTYLVDVPPEDLPPVHRRDLERAW
                     YAARDAALADRWSVVRGFRFRREDGSFSDLALADRDACCWAGAVDRTLGMRNSYGLSV
                     CIRLLALVDLLARARWADSFCLLQRDGAELHPVLLRAAASTPLTAEARFDETGFRGRL
                     SRFTFGMALEGQTAARLTGATP"
     gene            6937..7272
                     /locus_tag="VKQ27_17955"
     CDS             6937..7272
                     /locus_tag="VKQ27_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90872.1"
                     /translation="MICRAALLLPLLGLTYCAPPPPPVGQARADAATLAACREHANQV
                     YNRQNRDQIYTITNRDVPFSAGYAPGATDQGLAARYAHDNMVSDCVRNTGTETDRSAT
                     YTPDATVTP"
     gene            7457..7807
                     /locus_tag="VKQ27_17960"
     CDS             7457..7807
                     /locus_tag="VKQ27_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY90873.1"
                     /translation="MAHIKRTDPGAILSKVVEYHNFVYLQGVTAKDTNKDIKGQTADV
                     LAQIDHLLEEHGTDKTRLLQALIWVRNIKDRDAMNEVWTPWLPKDGAPARACVQAELA
                     NPSILVEIMVTACK"
     gene            8755..9411
                     /locus_tag="VKQ27_17965"
     CDS             8755..9411
                     /locus_tag="VKQ27_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HLY90874.1"
                     /translation="MGVHTATLHARIAGHSRYAYRGGISCVPYARAASGITVSGNAWQ
                     WWDNAAGVYARGSVPEPGSVLTFRSNGRMRLGHVAVVSSVINSREIEIDHANWWGAGM
                     SGGVAHNIPVVDVSDNNDWTAVRVGLGQSGDFGSVYPTYGFIYDRPDNGVVVASAGAP
                     APQPELNPAPSDLRSISERGWQTYEEVAEAPPAIKYHGRVVHVVSRRVTPVSHHRRQQ
                     "
     gene            complement(9530..9670)
                     /locus_tag="VKQ27_17970"
     CDS             complement(9530..9670)
                     /locus_tag="VKQ27_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90875.1"
                     /translation="MACDWVPTAPVPAELLAPVVAELLEPAVWAKDKVTEPTQRAAAS
                     VP"
     gene            complement(9719..9865)
                     /locus_tag="VKQ27_17975"
     CDS             complement(9719..9865)
                     /locus_tag="VKQ27_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90876.1"
                     /translation="MLVPDVLVPEPDPVPFRRPLLVPGMEDWVPPDPMPDGSVPGLAI
                     GAPE"
     gene            complement(9913..10440)
                     /locus_tag="VKQ27_17980"
     CDS             complement(9913..10440)
                     /locus_tag="VKQ27_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLY90877.1"
                     /translation="MTTAVVTLAVVFMQFSLLAFGGTNAVIPEVQRQVVQVHHWLTAR
                     EFASLFALAQAAPGPNMLVVTLIGWRVAALPGALATTLGVAGPSSIVTFFAYRIWHRF
                     RDAEWRRMVQRGLMPVTAGLLMASATLLISTTSVEWGTAAVTIAAALLLLFTRIHPLL
                     ILATAAALGASGILT"
     gene            complement(10437..10985)
                     /locus_tag="VKQ27_17985"
     CDS             complement(10437..10985)
                     /locus_tag="VKQ27_17985"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLY90878.1"
                     /translation="MPNEPASPPSLIQLFLGFLSIGMYGFGGVLPWARRMIVEQKRWL
                     SASEFTDMLGLCQFLPGGNIMNVTIALGARFHGVAGSVVAFVGLMAAPFTTVIMLGLV
                     YDRYGDLPAVQHAFAGLAAAASALVLATALKIAVPLRTRPFGIGVAVLTFVAIALLRL
                     PMVLVLPVMAGLATLLVWRFRE"
     gene            11099..11935
                     /locus_tag="VKQ27_17990"
     CDS             11099..11935
                     /locus_tag="VKQ27_17990"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF012306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retroviral-like aspartic protease family
                     protein"
                     /protein_id="HLY90879.1"
                     /translation="MPIDLGNGNLLVSVQVNGSPATFILDTGAERTLMGENVVRQLGL
                     DRDSWVASTVLGIGGYENRPNALPRALQLGGLTLRRHTLLEDHSVTVGPLPLAEIAGR
                     QIAGLLGRDFLSPFDLDINVPARELTLYDVSGCTTGFLPWRSTYASVPATTPVGAALV
                     IPVFIDGRPLHALIDTGASSSLVAAPGMARLGLTPALLERDPGGNGSGIGPAPVFMRR
                     HRFETLNVGPDTTTSPQLWVSAIHVVPIVDMLLGADWLRTRRVWLSFTTKQIFIAMRA
                     KG"
     gene            complement(11945..>12221)
                     /locus_tag="VKQ27_17995"
     CDS             complement(11945..>12221)
                     /locus_tag="VKQ27_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90880.1"
                     /translation="YAVDQQVPTSRHSMFLRANWVDAFVPYLNLTAIANVSLIDGSGF
                     VQGNADYYLSQHWTIGGLGSFTFGTRRSEFGSLPQVGTILLRIVRYM"
BASE COUNT         2151 a         4358 c         3680 g         2032 t
ORIGIN      
        1 agcgggatgt cgcgacatgg tgtcgctcaa ttcaatggtc cctcgcccgg gaaagagcgt
       61 gctcttctcg aagtagccgg tttcactggc ggccttgagt atcgaaacct cagacgcggt
      121 ttcggtcgcg gacgagctgg acatctttgg ttaatcctct cgtgcgtccg gtatagcaca
      181 ctcgaccgca ccgcatccgc ctcgcccctt gcatccactg ccactgcacg ttcatctgtg
      241 ccggcgatgc ccgctgctcc accaaccctc gccgccaacg tcatgcattt cgcgcgcctg
      301 ttgcggcgtg cggggctgcc ggttggtccg gcggagacac tggcgggaca gcaggcgctg
      361 tcgctgatcg atctcggcga ccgcatccag gcgcgcacgg cgctgcgcgc ggcaatggtc
      421 caccgccacg aacatcagga cgtgttcgac cacgccttca acctgttctg gcgtgaccca
      481 accgcggccg agcaggccgc ggcaatggcc ctcctcgacg cgcagaagga caagcgcccc
      541 gagcgcccac cgccaggcag ccgccgcgtc gccgaggcac tcgccaggcc acgcgaaacc
      601 aaaccgcgtg acgacgaacc tccggtgatg gacgccgtcc tcaccgtgtc cgatcgcgag
      661 cgcctccagc aaatggactt cgaagcgatg agcgcggcgg aaatcgccga ggccaagcgc
      721 gagatccaac gcctggtgct gccgctcgac cttcgccgca cccgccgcct gcgcgccgat
      781 ccactaggcc cggtgaccga cctgcgccgc accatccggc aaagcctccg ccaaggcggc
      841 gagatcctga caatcgcacg cgaacgccat gtgacccgcc cgcctccgct cgtggtcctc
      901 tgtgacattt ccggctcgat gagccgctac gcacaaatcc tgctccactt cctgcatgca
      961 gtgaccaatg accgcgaccg cgtgcatgtc ttcctattcg gcacgcggct ttccaacgtc
     1021 acacgccagc tccggtatcg cgatcccgag gtcgcgtttc agatggtgtc ccagatcgtt
     1081 cccgactggt ccggcggcac ccgaatcggc gaggccctgg aaagcttcaa ccgcttctgg
     1141 gcgcggcgcg tgctgggcca gggtgccgtc gtcctgctga tcaccgacgg cctcgatcgc
     1201 gacggtgcgc gcgggctcgc cgacaacatg gatcggcttc accgttcctg ccggcggctg
     1261 atctggctca atccgctgtt gcgctggagc ggtttcgagc caaaatcaca ggggatacgc
     1321 gcaatgctgc cgcacgtgga cgatttccgc cccgtgcaca atctggccag cctgcgcgcg
     1381 ctggtcgatc tgctgtctcg cccgacgcct caacgaacgc ccttggcgcc ggcgagagag
     1441 cctgaaagga tggcatcatg aacatcgagg aattcgaaga cgtgctgagc actgccgccg
     1501 gctggcgcgc tgccggcgag caggtggcac tggccacggt gaccgagaca tggggaagtt
     1561 cgccgcgccc ggccggcagc cagatggcgg tgaccaagtc cgggaagatg gcgggttcgg
     1621 tgtccggcgg ttgcatcgag ggcgccgtcg ccgacgcggc aatgaagacg atcgacacgg
     1681 ggacgccgca gctgctggat tttggcgtga cacatgagcg cgcctgggaa gtcgggctgg
     1741 cgtgcggggg taaggtgaag gtgttcgtgg agaaactttc gtgacccccg aactcctttc
     1801 cgccctcgaa gcggccaagc gcgaaaagcg ccccgtggtg ctggccaccc gcctgcctga
     1861 cggcgatcag tgcctgttac ccgatccgtc cgcggccgcc gcgctcaacg ccgaagccga
     1921 gaaggtcctg caatcggacg aaagcggcac ggtgaagatc gacggcactg actggttcct
     1981 ccacgcccac aatccaccgc tccggcttgt cgtcgtcggc gcggtgcaca tcgcccaggc
     2041 cctcgttccc tttgccacac aatgtggctt tgcggttgtg gtcgtggacc cgcgccgctc
     2101 cttcgcctcg gacgagcgct tcccgaatgt gaccgtctcc accgcctggc ccgacgaagc
     2161 cttggacgag ctgaagcctg acgcgcgcac cgccgttgtc accctgaccc acgatccgaa
     2221 gctggacgac cccgcgctgg accgtgccct gaaatcgcgc gccttctata tcggctcgct
     2281 cggctcccgt cgcacccacg cggcccggct aaaacggctg cgcgagctcg gccacggcga
     2341 caacgcgctc cagcggattc agggtccagt tggcctcaat atcgaggcgg tcaccgctcc
     2401 cgaaatcgcc ctctcgatca tcgccgagct ggtcgcggtc cgccgtggcg cgcccctgcc
     2461 gcacaggcaa ccgctcgccg catgaaattc ggcccggtcc cgctcgacga agcccgcggc
     2521 gccatcatgg cgcactcgca gcgcgtcggt gaacgcatga tccggaaggg agcggtgctg
     2581 gacgagcccg ccctccttgc cttgcgggaa gccggccgcg acgaggtcat cgtggcgcgc
     2641 ctggagccgg gcgacgtgcc agaagacatc gcagccgatc gtcttgccga accgctgatc
     2701 tcgccattgc ttgcgcggac gcgcgccgcc accggccgtg tcaatctgac ggcagaggca
     2761 cccggactgt tgcgggtcaa cacggcgatg atcaaccggc tgaacacgat cgacgagtcg
     2821 atcaccctcg gcaccttacc cgactacgct gtggtcgctc cgcgtgacct cgtcgccacc
     2881 gtcaagatca tccccttcgc ggtcagcaga accgtgctga cagttgccga ggccctggtg
     2941 cgccaggccg ggtctccgtt gacgctccat ccgttccgcc acctgaaagt tggactcgtc
     3001 gtcaccgaac ttcccggtct gaaggacagc gtcactgaga agacaatcgc agccaccgaa
     3061 cagcgtgtga cccagcttac cggcacgctt ctgccgcccc tccacacgcc ccatgaagag
     3121 gtgcccattg gcagggcgct ggaaaggctg atcgagcagg gcgccgatgc gttgttggtg
     3181 gttggcgcgt cggcggtggt ggacaagcgg gacgtcggtc cgatgggcat cgttcaggca
     3241 ggtggcgaga tcctgcattt cggtatgccg gtagaccccg gcaacctgat ctgcctcggc
     3301 cgtatcggca cccgcccggc cctggtgctg cccggctgcg cccgcagccc caagctgaac
     3361 ggcatcgact tcgtgctgag ccgcctgttc gccgacctgc cgatggggcc gggcgaaatc
     3421 atgcggatgg gcgttggtgg gctgctcaag gaaatcgaca tccggccgct cccgcgggag
     3481 aaggcgccgg ccacgccccg cgtcggtgcc gcgccgcgca gcgctccaac catcgccgtc
     3541 atcgtgcttg ccgctggacg ctcccgccgg atggcgccgc acaacaagct gctggttgtc
     3601 gatcgcaccg gcaagccgat gatcgcgcgc gtggtggaca acgtgctgtc gtcacacgcc
     3661 cggccgattc tggtcgtgac cggccatcag gccgaacagg tgcagcacgc cctcggcgga
     3721 cgccccgtgc gctatgtcca cgcggaggac tatgccgaag ggctgtctgc cagcctgaaa
     3781 gcgggcatcg ccgctgttcc tgaggaatgc gcagcggcgg tcgtgtgtct cggcgacatg
     3841 ccgctcgtca ccggccgcat gatcgatcgg ctgctctccg cctacgaccc cgacgagggc
     3901 cgcctgatcg tgttgcccac ctaccacggc aagcagggca acccgatgct atgggaccgc
     3961 gaattcttcc ccgagatcat ggacgtctcc ggcgactcgg gcgcgcgctt cctggtaggc
     4021 cagcacatcg aggcagtcgc cgaagtcgag atggccgacg acgccatact gcgcgatttc
     4081 gacaccaccg agtcactcag caccctgccc cagcggctgc gtccggaagg ggtcggctag
     4141 cttgcctatc tgcgaagcag atccctggcg cttccagtac ttcgagaagg tcgcctgccc
     4201 ggagcacgtc cgcatttcga ccgaggatcc ggactcgtgg gaatggttcc ccgaacatcg
     4261 ttggatttac gacaaactgg ctgtcgccct gtcgcaaggt ctccaggccg cccctcatgg
     4321 cgtgatgccg ccttctttcc cgattttttc caagccgatc acgaacctgc gcggcatggg
     4381 cacaggcagc caggtcatcc actcggccga cgagtaccgt accgccctca ccgctggcca
     4441 cttctggtgc acgctgctga ccggagccca tgtcagcacc gatgtggcac tagtggatgg
     4501 cgaaccacgc tggtggcgtc acaccacggg cgtcactgcc accggcggca cgttcgatta
     4561 ctggctcgtc caggccgcac cgatacccga gatcgaggac tggtgcggcg cctggtcacg
     4621 ccagcacctg cgcggctaca ccggcatggc taatttcgag accatcggcg gtcgcatcat
     4681 cgaagcacat cttcgctttg ccgaccaatg gcctgatctc tatggaaaag gttgggtcga
     4741 ggctgtggtc gacctgcacg ccactggcga ctggacctat ccagacggcg accgcaccga
     4801 cggctacagc gtcgtactgt tcggccccca cggcccccga tatcgccatc cgccggacag
     4861 gctggtgcaa gaggttctcg ccatgcccgg ggtttccagc gtgcagatca ccttccatga
     4921 ggacaaggcg cccgagcacc acgccatgcc tccgggcggc ttccgtcttg ccatcgtcaa
     4981 tgccgtctcg ctggacgctg gccgcgtcgg gcgcgcaaaa ctaggcgctg cacttctggg
     5041 ctgacgaagc ggcgccagtc acccaatcgc cgcggatacc cattgcgccc aggccagtgc
     5101 ctgccggtcc tcgccgatcc gcgccacgat ctggatcccg agtggcagcc cgttcggacc
     5161 ggtccccgcc ggcacagtca cgcaggggac gtgcagactg gtccagatca ggttgaaaac
     5221 cggatcgccc gtccactcca acccggccgg cgcctctccc ggcgccgacg gcgtgaccag
     5281 cacatccagc ccctccatac agcctggaag cgcatactgc gcacgctcga acgtggcatg
     5341 cgcctcgagc acctccgtct cactccgcga caggccgaac tcgagccgct ccctgagccc
     5401 gtcactgatc agctcgggcg catgcaccag ttcccagccc atcgctcgac cgctctcgtt
     5461 gttcatgacg atcgtatgcg cctcggcaac cgccgcgact tccggcgaca actcgcggtc
     5521 caccacgctc gcccctgccc tcgccaatgc cgaggcaacc cgctccagca gatgctgtgt
     5581 ctccggcgcc gccttgtccc aggccggtga ccgacacatc ccgatccgcg gcgcatgctc
     5641 cggccgcacc tccggattgc caagattacg cccggccacc gccccggcga acagggcgca
     5701 gtccgcaaca gaacgagcca taacaccaac ggtatcaagg ctctctgcca tgagcttcat
     5761 gccaaaccgg ttgatcgtgg caaaggtcgg cttgaacccg accacgccac aataggcagc
     5821 cggccggatc acactgcctg ccgtctgcgt gccgtaggcc agcgggaaga agtaatccgc
     5881 cacacccgct gccgaaccgc tgctcgaacc acccggcgta tgcgccagat tatgcgggtt
     5941 ccctgtcggc cccggcttcc gggtggcgaa ctccgtcgtc accgtcttgc cgatgaccac
     6001 gccgcccgct gcacgcgccc aggccaccgc cgccgcatcc gctcgaggcc gccaccccga
     6061 ccagatcgga gagccatatt cacttggcat atcagcagta tccagcacgt ccttcacgcc
     6121 aaccgggagt ccgtgcaatg gccccggccg agctccgccc gcggcattca acgctgctgc
     6181 cgcgtcgaac caggcgaatg cccgcaccga tgcctcccgc tcggcgatcc gtgccaggca
     6241 cgcctccatc agcgcctcag gccgaagctt cttttcccgg atcagttgcg ccgcttcggt
     6301 cgctgtcagc cttgctggat cgcccacacg aattctcccg ttccccgcac tcacaggctg
     6361 gcgcaacagc acccgcgggt ccatcctatg aatgatgcaa ccgtccgaac atccagcgtc
     6421 cgttcacgtc ggtaccgggc ctgacggctc gctgacctac ctggtggacg tcccgccaga
     6481 ggacctgccg ccggtccatc ggcgcgacct cgagcgcgcc tggtacgccg cccgcgatgc
     6541 cgccctcgcc gaccgctgga gcgttgtccg cggctttcgc ttccgccgcg aggacggctc
     6601 attctccgac ctcgccttgg ctgatcgcga cgcgtgttgt tgggcaggag ccgtggatcg
     6661 cacgctgggc atgcgcaatt cctacggcct gtccgtctgc attcgcctgc ttgcgttggt
     6721 cgacctgctg gcccgtgccc gctgggccga ttcattctgc ctgttgcagc gggacggagc
     6781 cgaactgcac cccgtgctgc tgcgcgcggc agccagcacg cccctcaccg ccgaggcgcg
     6841 tttcgacgag accggcttcc gagggcgact gtcccgattt accttcggca tggcgctgga
     6901 aggacagacc gcagcgcgcc tgacaggagc cacgccatga tttgccgcgc cgccctgctc
     6961 ctgccgttgc ttggcctgac atattgtgcg cccccacccc cgccggtcgg acaggcgcgc
     7021 gccgacgcag ccaccctcgc cgcctgccgg gaacacgcga accaagtcta caaccgtcag
     7081 aaccgggatc agatctacac aatcaccaac cgcgacgtgc ccttctccgc aggctacgct
     7141 cctggggcga ccgaccaagg cctcgcggcc cgctatgccc acgacaacat ggtcagtgac
     7201 tgtgtccgca acaccggaac cgagacagat cgctctgcca cctacacgcc ggatgcaacc
     7261 gtcacgccct gatctggaca ctaggccagc ctgacagcga tcggcgacgc ctctttgcgc
     7321 agcacattgc gcggtgccgc tataccggca gcgtcacatg ctggttcccg cgccagtgga
     7381 tagcgagacg ctggcctgat gccgccgcat gcaataaaac gcgccggacc acagcaacca
     7441 gcggggagac cagccaatgg cacacatcaa gcgcaccgat ccgggcgcaa tcctttccaa
     7501 ggtcgtcgag tatcataatt tcgtttatct acagggtgtt acagcgaaag acacgaataa
     7561 ggacatcaaa gggcagacgg ccgatgtgct ggcgcagatc gaccacttgc tggaagagca
     7621 cggcaccgac aaaacccgcc tgctgcaagc cttgatctgg gtgcggaaca tcaaggaccg
     7681 cgacgcgatg aacgaagtct ggacaccgtg gctgccgaaa gatggcgccc ctgcccgcgc
     7741 ctgcgttcag gccgagctgg cgaacccttc gatcctggtg gaaatcatgg tgaccgcctg
     7801 caagtgatcg ggcggccccg gtctgcaaac gcgcactgag gggagtgggc ggagtagctg
     7861 gcactaggca ttgcggtcag atcggcgata cagccagtca aacttgccag acagcggaaa
     7921 tgacaactcg ccagcgcccg ccagctcgcg tcggacagtc gttccagcgc gcacgagaca
     7981 atcggcgcga ccgcccgacc gggggttcgc gcgggcgcag ctgcttcttc aggccctgat
     8041 cacctttcct cggataggaa gaaccgcctt cgaagcatta cgctctaagg cgccagatca
     8101 ccgcacgcgc ctatcccgtc atgacggggc agacatcggc gccccgtccg ggctcgtccc
     8161 catgtgtgaa tagcgccgcc acactgttgc tgaaatacca ccacaccctg cgcgccttct
     8221 gctacacact cgtccgcgac cgcgtcctcc ccaaccgacg cgcaactgta acatgtgaag
     8281 ggacaccgcc gacgatcggc tcggttgtcc gtggtgccgc agattctccc tgcgatcagc
     8341 gtttgagtgc cgactccggg ccgcagaggt tgtcctgggc tcagattcgg acgccctgag
     8401 cagcgaaggg ttgtctgtta ctttccgatc atatcgggaa caaatccgcc atttaggata
     8461 acaatcccca gaatatcact catccacagg cctggatggg cgaaatgctg caccgcaagc
     8521 gaaatccccc ttcggcgggt tgtcatcatt gcaaccctgt tgctaccgaa tcacagagct
     8581 tgaaaacggg tgggggagca agccgcgatg cggggttggg gcaccggtgc taggactttg
     8641 tttttaggga cttttttcgc ggttgcgagt caactcaccg ggacgcccgc acacgctggg
     8701 gtgaatgccc agcacgcaac ccattcatca caatggcggc atggcaacgt tcggctgggc
     8761 gttcacaccg ccacactgca tgccaggata gctggtcatt cgcgctacgc gtatcgcggc
     8821 ggcatatcct gcgtccccta tgcccgcgct gcctccggca tcaccgtctc cggcaacgca
     8881 tggcaatggt gggacaacgc tgccggcgtt tacgctcggg gcagtgttcc cgaaccaggc
     8941 agcgtgctca cgttccgctc caacgggcgc atgaggcttg gccacgtcgc tgtggtcagc
     9001 tcggtcatca actcccgtga aatcgagatc gaccacgcca actggtgggg cgccggcatg
     9061 agcggcggcg tagcccacaa catccccgtg gtggacgtgt ccgataacaa cgattggacc
     9121 gctgtccgcg tcggactcgg ccagagcggc gacttcggca gcgtgtatcc gacctatggg
     9181 ttcatctatg acaggcccga caacggcgtc gtcgtcgctt ctgccggcgc acccgcgccg
     9241 cagcccgagc tgaaccccgc gccttccgat ctgcggtcta tctcagagcg cggctggcag
     9301 acttacgagg aagtcgccga ggcgccgccc gccatcaagt accacggccg ggtcgtgcac
     9361 gtggtgtcgc gcagggtcac gccggtgtcg catcatcgcc ggcagcagta atcgccgccg
     9421 aacatacccg ttcgcctgac gcgtttccgt gaggcgcggg acgcgagtta gcagttggcg
     9481 ttccttctct ggttcatgga aggagccgtc caatgcgcag attcgttcgc tacggcacgc
     9541 ttgcggcagc cctctgggtt ggttcggtga ccttgtcctt cgcccagaca gcgggctcca
     9601 gcaactccgc cacaacaggc gccagcagtt ccgctggtac cggcgcggtc ggcacccaat
     9661 cgcaagccat gggcacgagc gccagcagca acaccaatac cggcaggatg tcccagactt
     9721 actccggtgc gccgatggcc aatcccggaa cgctgccgtc gggcataggg tctggcggca
     9781 cccagtcttc cattccaggg accagcagcg gccgcctgaa cggcacggga tcgggctccg
     9841 gcacgagcac atccggcacc agcacaccgt cagccgcggc cggccgcccg tgcggtctgt
     9901 cgctgtccga ccctacgtca ggatccccga cgcccctaac gcagccgctg tcgccaggat
     9961 cagcagtggg tggatgcgag tgaacagcaa cagcagcgca gccgcgatgg tcaccgctgc
    10021 tgttccccac tccaccgacg tcgtgctgat cagcagcgtg gccgaagcca tcagcagccc
    10081 cgctgtcacc ggcatcagcc ctcgctggac cattcgccgc cactcggcgt cgcggaaccg
    10141 gtgccaaatc cggtaggcga agaacgtcac gatcgatgat ggcccggcca cgccgagcgt
    10201 tgtcgccagc gctcccggca gcgcggccac gcgccagccg atcagcgtga ccaccagcat
    10261 gttcggtccg ggtgccgcct gcgccagcgc gaacagcgag gcgaattcgc gcgcagtcag
    10321 ccaatggtgc acctggacga cctgacgctg cacctctggg atcaccgcat tggtgccgcc
    10381 gaacgccagc agcgagaact gcatgaacac cacggcgagc gtcacaacag ccgtggtcat
    10441 tcgcggaacc gccagaccag cagcgtggca agaccagcca tcactggcag caccagcacc
    10501 atcggcagcc gcaatagggc gatcgccacg aaggtcagca ctgcaacgcc gataccgaat
    10561 ggccgcgtcc tgagcggtac cgcgattttc aacgcagttg ccagcaccaa cgcagacgca
    10621 gccgccgcca gcccggcgaa ggcatgctgc accgccggca ggtcaccgta gcggtcatat
    10681 accagcccca gcatgatcac cgtggtgaat ggagccgcca tcaggcccac gaacgccacc
    10741 accgatccgg caaccccatg gaaccgcgcc cccagggcga tggtgacgtt catgatgttg
    10801 ccgccgggca ggaactggca cagccccagc atgtcggtga attcactggc gctgagccag
    10861 cgcttctgct cgacaatcat ccggcgtgcc cagggcagta caccgccgaa cccatacatg
    10921 ccgattgaca ggaacccgag aaacagttgg atcagcgaag gtggcgaggc tggctcattc
    10981 ggcatccggg ctggatactt gagctgtcgc gatgaaacaa atccgcctgt gtatcgtcat
    11041 attgctcgcc acgcttggct cggcgcacgc ggcgtgccgc gtgactgcgc agacgaccgt
    11101 gcccatcgat ctcggcaacg gcaatctgct cgtcagcgtt caggtgaacg gctcgcccgc
    11161 taccttcatc ctggacaccg gcgcggagcg cacgctgatg ggggaaaacg ttgtccgcca
    11221 gctcggcctt gaccgcgaca gttgggtggc ttccacggtc ctcggcattg gcggctacga
    11281 gaaccgtccg aacgccctgc cccgcgcgct gcagctcggc ggcttaaccc tgcggcggca
    11341 taccctgttg gaggatcaca gcgtcaccgt cggtcccctc cccctcgcgg agatcgccgg
    11401 tcgccagatt gccggtctgc tcggccgcga cttcctttcg ccgttcgacc tcgacatcaa
    11461 tgtgccggcc cgtgagctca ccctttatga cgtaagcggc tgcacaaccg gcttcctgcc
    11521 ctggcgcagc acctacgctt cagttccagc caccacaccc gtcggcgccg cgctcgtcat
    11581 cccggttttc atcgacggcc gtcccctcca cgcactgatc gataccggcg cgagctccag
    11641 cctggtcgcc gcacccggca tggcgcggct cggcctgacg cccgcgctgc tcgaacgcga
    11701 tccgggaggc aacggctccg gcattggccc tgccccggtg ttcatgcgcc gccatcgctt
    11761 cgagacgctg aatgtcggtc ctgacacaac caccagtcct cagctctggg tctcggccat
    11821 ccacgtcgtg ccaatcgtgg acatgctgct cggcgccgac tggctgcgca cgcgccgcgt
    11881 ctggctgtcg ttcacgacga agcagatctt catcgcgatg cgggcgaagg gctgagcggc
    11941 gcactcacat gtagcgcact atccgcagca ggatggttcc cacctgcggc aggctgccga
    12001 actccgagcg ccgcgtgccg aacgtgaagc tccccagccc accgatcgtc cagtgctgcg
    12061 acaggtagta gtccgcgttt ccctgcacga aaccggagcc gtcgatcagg ctgacattcg
    12121 cgatcgctgt caggttgaga tacggcacaa acgcatcgac ccagttggcg cgcaggaaca
    12181 tcgaatgccg cgacgtcggc acctgctggt cgacggcgta c
//
LOCUS       DATGEM010000588         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_717810, whole genome shotgun sequence.
ACCESSION   DATGEM010000588 DATGEM010000000
VERSION     DATGEM010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717810"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VKQ27_18000"
     CDS             complement(<1..264)
                     /locus_tag="VKQ27_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90881.1"
                     /translation="MIARGRQGAASVEFAIIGLAFISLLMFALDAGWQMVIDAALGVG
                     GRAAARFGTTGATVAFGITPAPTNRNDSIVGLVILNSGNLLRAD"
     gene            complement(261..995)
                     /locus_tag="VKQ27_18005"
     CDS             complement(261..995)
                     /locus_tag="VKQ27_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90882.1"
                     /translation="MKRLMLTASLALLVGCTAPDTGRRAGAAGDLRVADAAMASGLPQ
                     TALSMTRDILQTDPRNVGALLRQGAALAAMGQTDAAEECYRRVVEIDPRSADGLRGLG
                     RAALGAGDARKAEAMFRQAIEVTPGDAMALNNLGIALDLQERHEEAQASYHAALKQQP
                     AMTAARVNLGLSLALGGAPDRAVEILRPLASDPGADSKVRENLAVALALAGEREAAGK
                     VLAADMPQDTASVALAGYAALKVPAK"
     gene            complement(1007..1942)
                     /locus_tag="VKQ27_18010"
     CDS             complement(1007..1942)
                     /locus_tag="VKQ27_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLY90883.1"
                     /translation="MSGTALIAMLFAAALFVLAGVILNLQLKRDAKLAVRVRVVQRTV
                     GIEAVVGGAGASQPRLLRIVAQIGDVITHGGLLSSAMLEDLELRLNQAGFRGRNVLAP
                     FIGAKLLSLIGLPLLGWLVADRAGWPISMRLLVIAGAGLAGLLLPDYVVRLLHRRHLT
                     AVERGLPDALDMMVICTEAGLGLEPAIDRVGREIAATHADVAEELLNAAREMRVNADR
                     RLALMNMAARAQLPSLRRLAVTLVQTLQYGTPLSQALRTLSSEMRQEALTRFEARAGR
                     LPALLTVPMIVFILPCVFMIVGGPAIVHVMQAMRQ"
     gene            complement(1939..2907)
                     /locus_tag="VKQ27_18015"
     CDS             complement(1939..2907)
                     /locus_tag="VKQ27_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLY90884.1"
                     /translation="MTSAASLQLAALSLLLVSIGAASIPLMRADQRHRKLCDRIEAHA
                     TPYARATLVEARDKTNVTAAMSNGHVLLQLLIRLVAFDPAHRSYYPLPWWIVLPMGLV
                     FARLVVALAQTLFGSSMLLALPVVWLFSVRQFYRWCEGTRLRTLFEQFPDALAMLVRA
                     VRVGIPVTEGMRNIAADSPEPTRTEFAQVADQLSLGMTLDHALEGLAARNQLPEYGFF
                     ATALSLQSETGGTVSDTLERLADVIRKRVSLREHARALASEARASIMILAALPVCTGL
                     ALAVINPDYISTLFIEPQGRRIFTLAIGSLCTGVFTMRTIVSRSLR"
     gene            complement(2904..>4102)
                     /locus_tag="VKQ27_18020"
     CDS             complement(2904..>4102)
                     /locus_tag="VKQ27_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HLY90885.1"
                     /translation="KLTPEELTQEVERTLAEIATQRRIQLNGREQRALARELVNDMLG
                     LGPLEPLLEDDTITDIMVNGPDKVFVEKRGKLELSTVRFRDAGHVAHVCQRIAVRVGR
                     RIDESTPMVDARLKDGSRVNIVFPPLALDGPYVSIRKFSRHRINFAKLVEFGSLSAHA
                     AHLLEMAARARLNIIISGGTGSGKTTLLNALSSLIDIGERIVTIEDAAELQLQQPHVV
                     RMETRIASLEGRGEVTQRDLVRNALRMRPDRIILGEVRGTEAFDMLQAMNTGHDGSMS
                     TIHANTARDALTRIENMVQMGNMGLPARSILTQIVGAVDLIVQIERQRDGVRRVIQIT
                     DVCGMEGDIATLNDIFRFEVDGESLTGKIVGHYRCTRTRPSFHERLVYAGVERAWATM
                     MDEASK"
BASE COUNT          754 a         1492 c         1176 g          680 t
ORIGIN      
        1 gtccgcccgc agcagattgc ccgaattcag gatcaccaga ccgacgatcg agtcgttgcg
       61 gttggttggc gccggcgtga tgccgaatgc aaccgttgca ccggtcgtgc cgaaccgcgc
      121 cgccgctctc ccaccaacac ccagcgccgc gtcgatcacc atctgccagc cggcatccag
      181 cgcgaacatc agcagcgata tgaacgcgag cccgatgatc gcgaactcga ccgacgccgc
      241 accctgccgg ccgcgcgcga tcatttggct ggcaccttca gcgctgcata gcccgccagc
      301 gcgaccgagg cggtgtcctg gggcatgtcc gccgcaagca ccttgcccgc cgcctcacgt
      361 tcgcctgcca gagccaaggc gaccgccagg ttctcgcgca ctttgctgtc tgcccccgga
      421 tcgctcgcca atggccgcag gatctccacg gcccgatctg gcgctccacc cagcgccagg
      481 gacaggccca ggttcacgcg cgccgccgtc atcgcaggct gctgcttcag cgccgcatga
      541 tacgatgcct gcgcttcttc atgacgttcc tgcagatcga gcgcgatgcc cagattgttc
      601 agcgccatcg cgtctccagg cgtcacctcg atcgcctggc ggaacatcgc ctcagccttg
      661 cgcgcatcgc ccgccccaag cgccgcccga cccaacccac gcagcccgtc cgccgaacgc
      721 gggtcgatct caaccacccg gcgatagcat tcctctgcgg cgtcggtctg tcccatggcc
      781 gccagcgccg caccctgccg caacagcgca cccacattgc gtggatcggt ctgcaggatg
      841 tcgcgcgtca tgctcagcgc cgtctgcggc agaccactcg ccatcgcagc atcagcaacg
      901 cgtagatcgc ctgccgcgcc cgcccgcctg cctgtatccg gtgccgtgca tccgacgagc
      961 agtgccaacg aggccgtcag catcaggcgt ttcatggtta tgctccttat tgccgcatgg
     1021 cctgcatgac gtggacgatg gccggaccac caacgatcat gaacacgcac ggcaggatga
     1081 agacgatcat tggcaccgtg agcagggccg gcagtcgccc ggcccgtgcc tcgaaccgcg
     1141 tcagcgcctc ctgacgcatt tcgctggaca gcgtgcgcag tgcctggctc agcggcgtcc
     1201 cgtattgcag cgtctgtacc agtgtcaccg ccagccgccg taacgaaggt agttgcgcgc
     1261 gtgccgccat gttcatcagc gccaggcgcc ggtccgcgtt cacccgcatc tcgcgtgccg
     1321 cgttcaacaa ctcctcggcg acatcggcat gggtggcggc gatctcgcgg cccacgcgat
     1381 cgatcgccgg ctccagcccc aatcctgcct ccgtgcagat caccatcatg tcgagcgcat
     1441 ccggcaaccc gcgctcgact gccgtcagat gccgccgatg cagcagccgc accacgtaat
     1501 caggcagcag cagaccagcg aggcccgcac ccgcgatcac cagcaaccgc attgatatcg
     1561 gccatcccgc gcgatccgcc acgagccagc ccagcagcgg cagcccaatc agcgacagca
     1621 gtttggcgcc gatgaacggc gcaagcacgt tcctgccacg aaaccccgcc tggttcaggc
     1681 ggagttccag atcctccagc atcgccgaag acagcaaccc gccgtgcgtg atcacgtcac
     1741 cgatctgcgc cacgatacgc agcaaacgcg gttgcgacgc accagctccc ccaaccacag
     1801 cctcgatacc gacggtccgc tgcacgacgc gcacgcgcac cgccagcttc gcgtcgcgct
     1861 tcaactgaag gttgaggatg acgccggcaa gaacgaacag cgccgccgca aacagcatcg
     1921 caatcaacgc tgtcccgctc atcggaggct ccgcgatacg atcgtgcgca tcgtgaagac
     1981 gcccgtacac aatgacccga tcgccagcgt gaagatgcgc cgcccctgcg gctcgatgaa
     2041 cagcgtgctg atgtaatccg gattgatcac ggccagcgcc agtccggtgc acaccggcag
     2101 agccgccagg atcatgatgc tggcgcgcgc ctccgacgcc agtgcacgtg catgctcgcg
     2161 cagcgacaca cgctttcgga tcacatccgc caagcgttcc aatgtgtcac tgaccgtgcc
     2221 gcctgtctca ctctgcaatg agagcgctgt ggcgaagaag ccatactccg gcaactgatt
     2281 ccgtgcagcc agcccctcca acgcatgatc cagcgtcatc ccgagcgaaa gctgatcagc
     2341 cacctgcgcg aactcggtcc gcgtcggttc gggactgtcg gctgcgatgt tacgcatgcc
     2401 ctctgtcacc ggaatgccga cacgcaccgc gcgcaccagc atcgccaacg catcgggaaa
     2461 ctgctcgaac aacgtacgca ggcgggtgcc ctcacaccag cgatagaact gccgcacgga
     2521 gaacagccag accaccggca gcgccagcaa catcgaggac ccgaacaggg tctgcgcgag
     2581 cgcgaccacc aggcgcgcaa agaccaggcc catcggcagc acgatccacc aaggcagcgg
     2641 ataatacgaa cggtgcgccg ggtcgaacgc aaccaggcgg atcagcaatt gcagcagcac
     2701 atggccattg ctcatcgctg ccgtcacgtt ggtcttgtcg cgtgcttcca ccagcgtggc
     2761 gcgcgcatac ggcgtggcat gcgcctcaat ccggtcgcac agcttgcggt ggcgctgatc
     2821 ggcccgcatc agcgggattg acgcggcgcc aatgcttacg agcagcagcg acagcgcggc
     2881 caactgcagc gaggcggcgc tggtcatttg ctcgcctcat ccatcattgt cgcccacgcc
     2941 cgctccacgc cggcgtaaac cagccgctca tggaagctcg gccgcgttcg cgtgcagcga
     3001 tagtgcccga caatcttgcc ggtcaggctc tcgccgtcga cctcgaaacg gaaaatatca
     3061 ttgagagttg cgatatcgcc ttccatgccg cagacgtcgg tgatctggat cacgcgccgc
     3121 acgccgtcac gctggcgctc gatctgcacg atcagatcta ccgccccgac gatctgggtc
     3181 aggatcgagc gcgccggcag tcccatgttc cccatctgca ccatgttctc gatccgcgtc
     3241 agggcgtccc gcgcggtgtt cgcatgaatc gtggacatgc tcccgtcatg cccggtgttc
     3301 atcgcctgca gcatgtcgaa cgcttcggta ccgcggacct cgcccaggat gatgcgatcc
     3361 ggtcgcatcc gcagtgcgtt gcgaaccagg tcgcgctgtg tcacctcgcc acggccctcc
     3421 aggctggcga tccgcgtttc catccgcacc acatggggct gctgcaactg cagttcggcc
     3481 gcgtcctcga tggtgacaat acgctcgccg atatcaatga ggcttgacag cgcgttcagc
     3541 agcgttgtct tgcccgagcc ggttccgcca gaaatgatga tattcagccg cgcccgcgct
     3601 gccatttcca gcagatgcgc cgcatgtgcg ctcagcgagc cgaattccac caacttggcg
     3661 aaattaatcc ggtggcgcga gaatttgcgg atcgagacgt acggtccatc cagcgccagc
     3721 ggcggaaaca cgatgttgac gcgcgagccg tcctttagcc gcgcatccac catcggcgtg
     3781 ctctcgtcga tccgccgccc gacgcgcacc gcgatccgct ggcacacatg cgccacgtgc
     3841 ccggcatcac gaaaccgcac ggtcgaaagc tcgagcttgc cgcgcttctc gacgaacacc
     3901 ttgtccggcc cgttgaccat gatgtcggtg atcgtgtcat cctcgagcag tggctcgagc
     3961 ggcccgagcc ccagcatgtc gttgaccagt tcgcgcgcca aagccctttg ctcgcgtcca
     4021 ttgagctgaa tgcggcgttg cgttgcgatc tcggccagag tccgttcgac ttcctgggtc
     4081 agttcttccg gtgtcagttt cg
//
LOCUS       DATGEM010000589        10818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1065419, whole genome shotgun sequence.
ACCESSION   DATGEM010000589 DATGEM010000000
VERSION     DATGEM010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065419"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VKQ27_18025"
     CDS             complement(<1..617)
                     /locus_tag="VKQ27_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90886.1"
                     /translation="MATTEARVFLQRADALHAQRQLEAAAAEYRRAVALDATLFEAWY
                     GLGASLLSGGSHGAAAEALGRAVALRANADGARCNLAEALFQLGRVDEAVRHYELAAA
                     GGNAEVAQVSLDALACIVPGAMAVDNAGVLALRRRWAATQHVRALTPVAREAGRKLRV
                     GYVSAFFGDRNWMKPVFGVINRHDRSRFEIHLLSDGGDPAEQSGYV"
     gene            646..2919
                     /locus_tag="VKQ27_18030"
     CDS             646..2919
                     /locus_tag="VKQ27_18030"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HLY90887.1"
                     /translation="MDDDFRKAALDYHRLPQPGKLAIEPTKRMATQRDLSLAYSPGVA
                     AACEAIVADPDTARDYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKAVLFKKFA
                     GIDVFDIEVDAEDPTRFCDVVAALEPTFGAINLEDIKAPECFAIEAELRRRMCIPVFH
                     DDQHGTAITVAAAVRNGLVLQGKSLSDVRLVTSGAGAAALACVDLLVSMGLRPENVTL
                     TDIAGVIRTDRPGMLPNMARYARDTNAGTLPEVLAGADVFLGLSAPRVLKAEWLPLLA
                     DKPLILALANPEPEILPEQVTTHRPDAIVATGRSDYPNQVNNVLCFPFIFRGALDVSA
                     TTINEEMKIAAAEAIASLARMEASEVVAAAYGGDAPVFGPEYIIPKPFDPRLILEIAP
                     AVARAAMDSGVARKPIHDFAQYKRGLQRFVFRSGQLMAPIFEAAKQNPLRIVYGEGED
                     ERVLRAAQTLVDEGIAQPILLGRRAVIDRKIRDMGLRINLADGVAVIDPAEDQHIFAP
                     LLEDYQRLIGRRGIPPDDAAYGLTRRMTIAAAMLLHAGLADAAICGGSANWWRQVRYV
                     LPIIPRRLGVSRIYGLGCLILPHGTLFICDTQMVVDPTAEQIAEMTVLAAEAVSEFGI
                     TPKAALLSHSNFGASDSESARKMRNALALVRERAPELEVDGEMHADAALVAAIRDKAV
                     PDSRLSGTANLLVMPTLDAANISFTLLKAASDGLPVGPMLLGMSKPIHVLVPSVTARG
                     IVNLSAFAALCAANTVG"
     gene            2938..3753
                     /gene="fetB"
                     /locus_tag="VKQ27_18035"
     CDS             2938..3753
                     /gene="fetB"
                     /locus_tag="VKQ27_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HLY90888.1"
                     /translation="MNPILLSPYDIAIAAVLIVVDAALSLVLRLRLHTQLAIAAVRLV
                     VQLVAIGYILGLVFKVASPPLTLAVVLVMVAIAGREVAARPEQRLGRFGNYAVGASAV
                     AFATFLTATLALTTAIRPKPWYDARYAIPLAGIILGNVLNGSSLALDSMLGGVVRERA
                     AIEAQLCLGASFFQAMRGLIRDSVRRALLPIINQMSAAGIVTLPGIMTGQILAGMAPL
                     EAAKYQILLIFLLSGGSGLASVAVVYLAAMRLTDDRQRLRIDRLTAKPRGPHP"
     gene            3750..4463
                     /locus_tag="VKQ27_18040"
     CDS             3750..4463
                     /locus_tag="VKQ27_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLY90889.1"
                     /translation="MIASDVMTRNVISVPPDATIAEAVELMLERGISGLLVVDNSGTL
                     VGIVTEGDLLRRDEIGTRRHRSWWLRLIASPSRQAADFTRTHGRKVADVMTHDVLAVD
                     AKAPLTDIVALMEEHRVKRVPVLEGDRVVGVVSRADLLRALSVAAREHGAAAADDATI
                     RDRILTTLSRESWAPKTTLNVTVVNGVVDLWGTISNNQERRAICVIAENTPGAKKVID
                     HLVFVEAYTGTVIEPPPGA"
     gene            4527..5813
                     /locus_tag="VKQ27_18045"
     CDS             4527..5813
                     /locus_tag="VKQ27_18045"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLY90890.1"
                     /translation="MSFGRRNFVAMGAAAVLQACAAVPDVNEYLGNADPLQRHYAMQQ
                     ALDGGSLVFGNQTQLLKDGAQAFPAMFEAMAQARDHINLEYFIFEDVSSNGQQLSDLL
                     IDKLSHGVAVNIIYDAYGSQDTPGALFDGLRRAGAKVVEFNPINPLGALTGHSPNDRD
                     HRKIMVVDGHIGFTGGINLSKVYENPPSAGVPADGDTAHAYWRDTAIAIQGPAVAELQ
                     KLFFATWKKQNGPPIDQANYFPPLARQGVQTVRIIGSSPGDQRPLYFVSLEAAVRGAQ
                     QRIWLSTGYFVPPHQEREDVGKAARRGVDVRLVVPSHTDVQAAVYAGRAAYGDLLEDS
                     ARIYEMQNAVLHSKLATADGVWTAIGSSNLDRRSVVFNNEVDAIVIGSDTASQVEALL
                     QQDMAMSRQMSLQGWEHRPIGERLDELKARLWQYWM"
     gene            5854..6609
                     /locus_tag="VKQ27_18050"
     CDS             5854..6609
                     /locus_tag="VKQ27_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY90891.1"
                     /translation="MNIRFDGQTVAVTGAGHGFGRCIAQTFAHLGARVFATDISADEL
                     SETARDGGITTKVVNLRDRAAGAAWIHQIEAQTNGPIHALVNNAGGVGGQGPKPLEDV
                     TDEDWNMLFDINVNAAFTLCRAAAPAMKRAGGGRIINTSSGAGLQASLTGIQAYCSAK
                     HAVVGLTRQLAHEFGPYNITVNSVAPGFIRTNEATEKQWESYGPEGQKALVNRIALKH
                     LGSAQDIANAVVFFASDLAAFVNGQILQVDGGR"
     gene            complement(6646..7173)
                     /locus_tag="VKQ27_18055"
     CDS             complement(6646..7173)
                     /locus_tag="VKQ27_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90892.1"
                     /translation="MVPSITRKLVPNSPERARRMRKHLVQSLRALRTMKDPQGNASPV
                     RPAPEGFAAKVARTACTMCRGYCCKGGGEHAYLDERVMARVRIAEPELDARGVLRLYM
                     DSVPAVGYEGTCLFHGEKGCTLDRSLRSDVCNSYFCDMLGEFVKKADKATSAIVMSGE
                     GEFSRMSHILRPDRK"
     gene            7284..8207
                     /locus_tag="VKQ27_18060"
     CDS             7284..8207
                     /locus_tag="VKQ27_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLY90893.1"
                     /translation="MAIPIILDCDPGTDDAFALLLALASQEIAVLAVTVAGGNVGLDR
                     TLANARSLVALAGSRVPVYGGADRPLLGSFVNETRVHGVDGLGGIALPAGDPVAPGVA
                     ADAIRTHVRTNLEPVTLVGIAPATNIALALATDPAILENIERIVLMTGAWGEGNATPS
                     AEFNALNDPEALAVIFACGRPVVLATLELTAQALCTPARLAALRASGDGECLRALCDI
                     QATVPLGPRLGGKGAALHDPCALAWLIQPDLFTTRDCAASVELGPGPSRGRTVIDRWH
                     RTNAPHNVTLLESLDADGFFVLLAESFDRLP"
     gene            8347..9477
                     /locus_tag="VKQ27_18065"
     CDS             8347..9477
                     /locus_tag="VKQ27_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLY90894.1"
                     /translation="MNPADMPFDADTILDGLRTWIECESPTYDASAVNRMMDLASRDL
                     IVAGARIERVAGRMGFGDCIRATFPHSTPNQPGILVMGHLDTVHPIGTLEKLPFRRNG
                     SRAWGPGILDMKGGNYMALEALRQLGRAHIQTTLPVTVLFTSDEEVGSPSTRDLIEAE
                     AARNKYVLVPEPARADGGVVTGRYAIARFNLEATGRPSHAGARLTEGRSAIREMARKL
                     IEIEDMTTEDCTFSVGVIHGGQWVNCVTTTCTGEALSMAKRQEDLDRGVAAMLALGAS
                     SNDVQFRVTRGVTRPVWEPDAKVMALYEKARGIAEGLGYHIASQSSGGGSDGNFTGAM
                     GIATLDGLGVQGAGAHTLDEHIVVDSLAYRGKLMAGLLATLP"
     gene            9474..10154
                     /locus_tag="VKQ27_18070"
     CDS             9474..10154
                     /locus_tag="VKQ27_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HLY90895.1"
                     /translation="MSKVKLFARTWRAIRSRPRLFIGLLCGLAVAPLLPGELSRTTRS
                     IIAWDIGVVVYLVLAAFLFTTERLSRMAEDAAAQQEGEWTIFWLTVGVITFTFIAIIH
                     EFAHISKDMPPAQRNLHVALVAVTLFVSWLMTHTTFALRYAHEYYEVEHGAAGISGGL
                     DFPGEKRPDYLDFVYFSLVLGMTFQVSDVQITARKFRRMAAVHGLLSFLFNTIILALT
                     VNIAAGLL"
     gene            complement(10111..>10818)
                     /locus_tag="VKQ27_18075"
     CDS             complement(10111..>10818)
                     /locus_tag="VKQ27_18075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90896.1"
                     /translation="EGGRAGFGTPRVIGGFVVAAASGIGFLAAELCASEPMLPLGFFR
                     NATFSVATLVGLTINLTLYGMIFVLGLYLQQVLRYSPLQAGMAFLPFPVALGLANVAA
                     GHVAEQYRPRVRMAIGLGIAAVGFWLLSHVQGNSAYWPLLPGMIIIPIGIGLAVPLMT
                     ATLLSTVPRDRSGTASGVLNTVRQAGGAIGVALFGALLGAHGVAGMRVALVLSAMALL
                     AVGITAALPRYSRSMPG"
BASE COUNT         1869 a         3860 c         3324 g         1765 t
ORIGIN      
        1 acataacccg actgctcggc cgggtccccg ccatccgaca gcagatggat ttcgaaccgg
       61 gaccggtcgt gccggttgat cacgccgaac accggcttca tccagttccg gtcaccgaag
      121 aacgccgaca cgtagccaac ccgcagcttc cgccccgcct cccgcgccac cggcgtcagc
      181 gcgcgcacat gctgcgtcgc cgcccaccgc cgccgcagcg ccagcacgcc cgcattgtcc
      241 accgccatgg cacccggaac gatgcaggcc aacgcatcca gcgaaacctg cgccacctcg
      301 gcgttgccgc ccgcggccgc cagctcgtag tgccgcaccg cctcgtcaac gcgcccaagc
      361 tggaacaggg cctcggccag gttgcaccgc gcgccgtccg cattcgcccg caacgccacc
      421 gcgcgcccca gcgcctcggc cgcggcgcca tgcgatccgc ctgacagcag gcttgcgcca
      481 agcccgtacc acgcctcgaa cagcgtcgcg tccagcgcca ccgcgcgccg gtactccgcc
      541 gccgcggcct cgagctgtcg ctgcgcgtgc agcgcatcgg cacgctgcaa aaacaccctc
      601 gcttcggtcg ttgccatgga atcgcctccc gatcattgtg cgcggatgga cgacgatttc
      661 cgcaaggcag cgctggatta tcaccggctg ccgcagccag ggaaactggc gatcgagcca
      721 accaagcgca tggccactca gcgcgatctc tcgctcgcct attcccccgg cgtcgccgcc
      781 gcctgcgagg ccatcgtcgc ggacccggac accgcccgcg actacaccgc ccgaggcaac
      841 ctcgtcgcgg tgatctccaa tggcacggcg gtgctcggcc tcggcaatat cggcgcgctg
      901 gccggcaagc cggtgatgga aggcaaggcc gtcctgttca agaagttcgc gggcatcgat
      961 gtattcgaca tcgaggtgga cgccgaagac cccacccgct tctgcgacgt ggtcgctgcc
     1021 ctcgaaccca cgttcggcgc catcaacctg gaggacatca aggcgccgga atgcttcgcg
     1081 atcgaggcgg aactccgccg ccgcatgtgc atcccggtgt tccacgatga ccagcacggc
     1141 accgcgatca ccgtcgctgc cgcggtccgc aacggcctcg tgctgcaagg caagtcgctg
     1201 tccgacgtgc gcctggtcac ctctggcgcc ggtgcggcgg cgctggcctg cgtcgatctg
     1261 ctggtctcga tgggcctgcg cccggagaac gtcaccctca ccgacatcgc cggcgtcatc
     1321 cgcaccgatc gtcccggcat gctgcccaac atggcccgct acgcccgcga caccaacgcc
     1381 gggacgctgc ccgaggtcct cgccggcgcc gacgtctttc tcggcctctc cgcgccgcgc
     1441 gtgctgaaag ccgagtggct gccactgctg gcggacaagc cgctgatcct ggcgctggcc
     1501 aaccctgaac cggagatact gccggaacag gtcacgaccc atcgccctga cgcgatcgtg
     1561 gccaccggac gcagcgacta cccgaaccag gtcaacaacg tcctctgctt ccccttcatc
     1621 ttccgtggcg cgctcgatgt atccgcgacc acgatcaacg aggagatgaa gatcgccgcc
     1681 gccgaggcga tcgcctcgct cgcgcggatg gaagcctcgg aagtcgttgc cgcggcctat
     1741 ggcggcgacg ccccggtgtt cggccccgaa tacatcatcc cgaagccgtt cgatccgcgc
     1801 ttgatcctgg aaatcgcccc cgcggtcgcg cgcgccgcga tggacagcgg cgtcgcccgc
     1861 aagccgatcc acgacttcgc ccagtacaag cgcggactgc agcgcttcgt gttccgctct
     1921 ggccagctca tggccccgat attcgaggcg gcgaaacaga acccccttcg tatcgtctat
     1981 ggcgagggcg aggacgaacg cgtactccgc gccgcccaga cgctggttga tgaaggtatc
     2041 gcgcagccca tcctgctcgg ccggcgcgcg gtgatcgatc gcaagatccg cgacatgggt
     2101 ctgcgcatca acctcgccga cggcgtcgct gtgatcgacc cggccgagga ccagcacatc
     2161 tttgccccac tgctggagga ctatcagcgc ctgatcggcc gtcgcggcat tcctcccgac
     2221 gacgcggcct atggactgac ccggcgcatg acaatagcgg cggctatgct gctgcatgcg
     2281 ggcctcgccg atgcggcgat ctgcggcggc agcgccaact ggtggcgcca ggtgcgctac
     2341 gtcctgccga tcatccctcg ccgcctcggc gtctcgcgta tctacggact cggctgcctc
     2401 atcctgccgc acggcacgct gttcatctgt gatacccaga tggtggtgga tcccacggcc
     2461 gagcagatcg ccgaaatgac ggtgctcgcc gccgaagccg tgtccgagtt cggcatcacg
     2521 ccaaaagccg cgctgctgtc gcactcgaac ttcggcgcca gcgactccga aagcgcgcgc
     2581 aaaatgcgca acgcgctcgc gctggttcgc gaacgcgcgc cggagctgga agtcgacggc
     2641 gagatgcacg ccgacgccgc ccttgtcgcc gcgatccgcg acaaggcagt gcccgacagc
     2701 cgcctgagcg gcacggccaa cctgctggtg atgccaacgc tcgatgcggc gaatatctcc
     2761 ttcaccctac tgaaagcagc ctccgacgga ctgcccgtgg gccccatgct gcttggcatg
     2821 agcaagccga tacacgtgct tgtgccgagc gtcacggcgc gcggcatcgt caatctgagc
     2881 gcattcgccg ccttgtgcgc cgccaatacc gttggatagg cagcgcggcg tcgggacatg
     2941 aacccgatcc tgctctcccc gtacgacata gccatcgccg cggtgctcat cgtggtggac
     3001 gccgccttgt ccctggtact gcgtcttcgc ctccacaccc agcttgccat cgccgcggtg
     3061 cgcctggtcg tgcagcttgt ggccatcggc tacatcctgg gcctcgtttt caaggtcgcc
     3121 tcgcctccgc tcacgctggc ggtcgtgctg gtcatggtgg cgatcgccgg ccgcgaggtc
     3181 gccgcgcgcc cggaacagcg actgggccgc ttcggcaact acgccgtcgg cgcctcggcg
     3241 gtggccttcg ccactttcct caccgctacc ctggcgctga ccaccgccat ccgcccgaaa
     3301 ccctggtatg acgcgcgcta cgccatcccg cttgccggca tcatcctcgg caacgtgctg
     3361 aacggcagct cgctcgcgct cgactcgatg ctgggcggcg tggtgcgtga acgcgctgcg
     3421 atcgaggcgc agctttgcct cggcgccagc ttcttccagg ccatgcgcgg tctgatccgc
     3481 gactcggtcc gacgggcgct gctgccgatc atcaaccaga tgtcggcggc cggcattgtg
     3541 acgctgcccg gcatcatgac cggccagatc ctcgccggca tggcgccgct ggaggcggcc
     3601 aaataccaga tcctgctgat cttcctgctt tccggcggca gtggccttgc gtcggtcgct
     3661 gtggtttacc ttgcggcaat gcgcctgacc gatgatcgcc agagactgcg catcgatcgg
     3721 ctgaccgcca aacccagagg accgcaccca tgatcgcctc ggacgtcatg acccgcaacg
     3781 tgatctcggt gccgcccgac gccacgatcg ctgaggccgt cgagcttatg ctcgagcgcg
     3841 gcatctccgg tctgctcgtg gtggacaact ccggcaccct ggtgggcatc gtgaccgagg
     3901 gcgacctgct gcgccgcgac gagatcggca cccgtcgcca tcgctcctgg tggctgcgcc
     3961 tgatcgcctc gcccagccgc caggctgccg acttcacccg tacgcacggc cgcaaggtcg
     4021 ccgacgtcat gacccacgac gtgctcgccg tggatgccaa ggcgccgctc accgacatcg
     4081 tcgccctgat ggaggagcat cgggtcaaac gcgtgccggt gctggaaggt gatcgcgtgg
     4141 tgggggtggt cagccgtgcc gatctgctgc gcgccctctc cgtggccgcg cgcgagcacg
     4201 gtgccgcggc cgcggatgac gcgaccatcc gcgaccgcat cctgaccacg ctatcgcgcg
     4261 aaagctgggc accaaagacc acgctgaacg tcaccgtggt caatggcgtg gtcgatctgt
     4321 ggggcaccat cagcaacaac caggaacgcc gcgctatctg cgtgattgcc gagaacacgc
     4381 cgggcgcgaa aaaggtcatc gaccatctgg tgttcgtcga ggcctacacc ggcacggtca
     4441 tcgaaccgcc gcctggagca tagtcgcgca gacacatctc ggtgcgcggt cacagttcgg
     4501 ccgcaatgcc atagcccttc gcgcccatgt cttttggccg ccgcaacttc gttgcaatgg
     4561 gtgcggccgc ggtattgcaa gcctgcgccg cggttcccga cgtgaacgag tacctgggca
     4621 atgcagaccc gctgcagcgg cattatgcca tgcagcaggc gttggacggc gggtcgcttg
     4681 ttttcggcaa tcagacgcag ctgctgaagg acggagccca ggccttcccg gcgatgttcg
     4741 aggcaatggc gcaggcgcgc gaccacatca acctggaata cttcatcttc gaggacgtgt
     4801 cctccaacgg acagcaactc tccgacctgc tgatcgacaa gctgagccac ggcgtcgccg
     4861 tgaacatcat ctacgacgcc tatggctcgc aggatacgcc aggtgcgctt ttcgacgggc
     4921 tgcgccgcgc cggcgcgaag gtcgtggagt tcaatccgat caatcccctc ggcgcgctca
     4981 ccgggcattc acccaacgat cgcgaccatc gcaagatcat ggtggtggac gggcatatcg
     5041 gcttcaccgg cggcatcaat ctatcgaagg tctacgagaa cccgccatcc gctggcgtgc
     5101 ccgccgacgg cgacacggcc cacgcctact ggcgggacac cgcgatcgca atccaaggtc
     5161 cagctgtcgc cgagctgcag aagctgttct tcgccacgtg gaaaaaacag aacggcccgc
     5221 cgattgacca ggccaactac ttcccaccgc tggcacggca aggcgtgcag acagtgcgca
     5281 tcatcggcag ctcgcccggc gaccagcggc ctctgtattt tgtctcgctc gaagcagcgg
     5341 tgcgtggggc gcagcagcgc atctggctct ccaccggcta tttcgtgccg ccgcatcagg
     5401 aacgcgagga cgttggcaag gcagcccgcc gcggcgtcga tgtgcgcctg gtggtgccaa
     5461 gccacacgga tgtgcaggcc gccgtctatg caggacgcgc cgcctacggt gacctgctcg
     5521 aagacagcgc gcgtatctac gaaatgcaga acgccgtgct gcattccaag ctggcgaccg
     5581 ccgatggggt atggacagct atcggctcat caaacctcga ccgccgcagc gtcgtgttca
     5641 acaacgaggt cgatgccatc gtcatcggct ccgatacagc atcgcaggtc gaggcattgc
     5701 tgcagcagga catggccatg tcccgccaga tgtccttgca gggctgggag caccgcccca
     5761 tcggcgagcg cctcgacgag ctgaaggcga ggctttggca atactggatg tagttcgcta
     5821 gcgtggccac ttcctgtcgg atgaggttcg cccatgaaca ttcgcttcga tggccagacg
     5881 gtcgcggtca ccggtgccgg ccacggcttc ggccgctgca tcgcacagac cttcgcccat
     5941 ctcggcgcac gtgtcttcgc caccgacata tcggccgacg agctctccga aaccgcgcgc
     6001 gatggcggca tcaccaccaa ggtcgtgaac ctgcgcgacc gcgccgcggg tgccgcctgg
     6061 atccatcaga tcgaggcaca gacgaacggc ccgatccacg cattggtgaa caatgccggc
     6121 ggcgtcgggg gccagggacc gaagccgctg gaagatgtta ccgacgaaga ctggaacatg
     6181 ctgttcgaca tcaacgtgaa tgcggccttc accctgtgcc gcgctgccgc accggcgatg
     6241 aagcgtgccg gcggtggccg catcatcaac acgagctccg gcgcgggact gcaggcctcg
     6301 ctgaccggca tccaggccta ttgctccgcc aagcacgccg tggtggggct gacccgccag
     6361 ctcgcacacg agttcggtcc ctacaacatc acggtcaact cggtcgcccc cggcttcatc
     6421 cgcaccaacg aagcgaccga aaaacaatgg gagagctacg gcccggaagg tcagaaggcg
     6481 ctggtgaacc gcatcgcgct gaagcacctc ggctccgcgc aggacattgc caacgccgtg
     6541 gtcttcttcg cctccgatct ggcggcattc gtcaacggcc agattctgca ggtggatggc
     6601 ggccgataag cgcgtccctg gctcacggag tcattgcggg ggaacctact tccggtccgg
     6661 ccgcaggatg tgtgacatgc ggctgaactc gccctcaccc gacatgacga tggcgctggt
     6721 cgccttgtcc gccttcttta cgaactcacc gagcatgtcg cagaaatagc tgttgcacac
     6781 gtccgatcgc agcgaccggt ccagcgtgca gcccttctcg ccgtggaaca ggcaggtccc
     6841 ctcgtatccg accgccggca cgctatccat gtacagccgc aacaccccgc gcgcgtccag
     6901 ctccggctcg gcaatccgca cccgcgccat gacgcgttcg tcgagatacg catgttcgcc
     6961 gccgcccttg cagcaatagc cgcggcacat cgtgcacgcg gtgcgcgcga ccttggcggc
     7021 aaagccctcc ggcgcgggac gcaccggcga tgcattgccc tgcgggtcct tcatcgtccg
     7081 cagcgcacgc agcgactgca ccaggtgctt gcgcatccgc cgcgcgcgct cgggcgaatt
     7141 cggcaccaac ttccgcgtga tcgaagggac catgaccgcc tggtcaccgt ccccatcgga
     7201 atcctccgtg ggaaccgaga aatactgcat ggacatgacg ccgcagggtg gcatcctgat
     7261 cgaacagaaa caaggatttt agcgtggcga tcccgatcat cctggactgt gacccgggca
     7321 ccgacgatgc cttcgcgctc ctgctcgccc tggcgtcaca ggaaatcgcg gtgcttgcgg
     7381 tgacggtggc tggcggcaac gtcggcctgg accgcacgtt ggccaatgcg cggtctctgg
     7441 tcgcgcttgc cggctcgaga gtgcccgtct atggcggcgc cgatcgcccg ctcctgggct
     7501 ctttcgtcaa cgagacccgc gtgcacggtg tcgacggcct gggcggcatt gcgctgcccg
     7561 ccggcgatcc tgtggcacct ggcgttgccg cggacgcgat ccgcacccac gtccgcacca
     7621 acctggagcc ggtcacgctc gtcggcatcg cgcccgccac caacatcgcg ctcgcgctcg
     7681 cgaccgaccc ggccatcctg gagaacatcg agcgcatcgt cctgatgacc ggcgcctggg
     7741 gcgaaggcaa cgcgacgccg tcggccgagt tcaatgcgct gaacgatccc gaggcgctcg
     7801 cggtgatctt cgcctgcggc cgcccggtgg tgctggccac gctggaactc accgcgcagg
     7861 cactgtgcac accggcacgg ctggccgccc tgcgcgcctc cggcgatggc gagtgcctgc
     7921 gcgcgctgtg cgacatccag gcgaccgtgc cgctcggccc acgcctcggc ggcaaaggcg
     7981 ccgcgctgca cgacccctgc gcgctcgcct ggctgatcca gcccgatctg ttcaccacgc
     8041 gcgactgcgc cgccagcgtc gagctcggcc ccggccctag ccgcggccgc acggtgatcg
     8101 accgttggca ccgtaccaat gcgccgcaca atgtcacgct gctggagtca cttgatgccg
     8161 acgggttctt cgtcctgctg gccgagagct tcgatcgact gccctgaaga ttgcagagcc
     8221 cctgtcattg cgaggaggcg cagccgacga atcaatctcg aggaagtcgt ggcaccgagc
     8281 ccacgcgacc gagatcgctt cgtcggctgc gcctcctcgc aatgacaacg ttgaaggata
     8341 ccgtccgtga acccagccga catgccgttc gatgccgaca ccatcctgga tggcctacgc
     8401 acatggatcg aatgcgaaag ccccacctac gacgcctccg ccgtcaatcg catgatggac
     8461 ctcgcctcgc gcgacctgat cgtcgccggt gcgcgcatcg aacgggtcgc cggacggatg
     8521 ggcttcggcg actgcatccg tgccaccttc ccgcacagca cgccgaacca gccgggcatc
     8581 ctggtgatgg gccatctcga cacggtgcat cccataggca cgctggaaaa actgccattc
     8641 cggcgcaacg gcagccgagc ctgggggccc ggcatcttgg acatgaaggg cggcaactac
     8701 atggcgctgg aggcgctccg ccagctgggc cgcgcgcata tacagacaac actgccggtc
     8761 accgtgctgt tcaccagcga cgaggaagtc ggcagcccct cgacccgcga tctgatcgag
     8821 gcggaagccg cccgcaacaa atacgtgctg gtgcccgaac ccgcgcgcgc ggatggcggc
     8881 gtggtgaccg gccgctacgc catcgcgcgc tttaaccttg aagccaccgg ccggccgagc
     8941 cacgccggtg cgcgcctcac cgaaggccgc tccgccattc gtgaaatggc gcgcaagctg
     9001 atcgagatcg aggacatgac caccgaggac tgcacgttct ccgtcggcgt catccacggc
     9061 ggccagtggg tcaactgcgt caccaccacc tgcaccggag aagcgctgtc gatggccaag
     9121 cgccaggaag acctggatcg tggcgtcgcc gccatgctgg cgctcggcgc atcgtccaac
     9181 gacgtccagt tcagggtgac gcgcggcgtg acccgcccgg tctgggaacc cgacgcgaaa
     9241 gtgatggcgc tgtacgagaa ggcgcgcgga atcgccgagg gcctcggcta ccatattgca
     9301 tcgcaaagct cgggtggcgg atcggatggc aatttcacgg gcgcgatggg catcgcaacg
     9361 cttgatggtc tcggcgtgca aggagccggt gcccacacac tcgatgagca tatcgttgtg
     9421 gacagcctcg cctatcgcgg caagctgatg gcggggctgc tggcaacgct cccttgagca
     9481 aggtcaagct gttcgctcgt acctggcgtg ccatccgcag ccggccgcgg ctgttcatcg
     9541 gcctgctgtg cggccttgcc gttgcacctt tgctgcccgg tgaactgagc cggaccacgc
     9601 gttcgatcat cgcatgggat atcggtgttg tcgtttacct tgtgctggcc gccttcctct
     9661 tcaccaccga acgcctgagc cggatggcgg aggatgcggc cgcacagcaa gaaggcgagt
     9721 ggacgatttt ctggctgacg gtcggcgtca tcaccttcac cttcatcgcc atcattcatg
     9781 agttcgccca catcagcaaa gacatgccac cagcgcagcg caatctgcac gtagccctgg
     9841 tggcggtgac actctttgtc tcctggctca tgacacacac cacgtttgcc ctccgctatg
     9901 cgcatgagta ttacgaggtg gagcatgggg ccgccggcat ttccggcggc ctcgacttcc
     9961 ccggcgagaa gcggccggac tacctggact tcgtgtattt ctcgctggtg cttggcatga
    10021 cgttccaggt gtccgacgtc cagatcaccg cgcgcaaatt ccgccgcatg gccgcagtac
    10081 acgggctgct gagcttcctt ttcaacacca tcatcctggc attgaccgtg aatatcgcgg
    10141 cagggctgct gtaatcccga cggcgagcag cgccattgcc gacagcacga gcgcgacgcg
    10201 catgcccgcg accccatgcg ctcccagcag agcaccgaac agtgcgacgc ctatggcccc
    10261 acccgcctgc cgcaccgtgt tcagaacgcc cgacgcggtg cccgaacgat cgcgcggcac
    10321 ggtcgagagc agcgtcgccg tcatcagcgg caccgccagt ccgatgccaa tggggatgat
    10381 gatcatgcca ggcagcagcg gccagtaagc actgttgcct tgcacgtgcg acagcagcca
    10441 gaacccaacc gccgcaatcc ccaggccgat cgccatccgg acgcgcggtc tgtactgctc
    10501 agcgacgtga cctgccgcga cattggccag tcccaacgcg acggggaacg gcaggaatgc
    10561 catgccggcc tgcaatggcg agtagcgcag aacctgctgt agatacaatc ccaacacgaa
    10621 gatcatgccg tagagcgtaa ggttgatcgt cagccccacc agcgtcgcca cgctgaacgt
    10681 cgcgttgcgg aagaagccca atggcagcat gggttcagac gcgcacaatt cggcggcgag
    10741 aaatccgatg ccgctcgccg ccgccaccac aaacccgccg ataacccgcg gcgtaccgaa
    10801 ccctgcgcgg ccgccctc
//
LOCUS       DATGEM010000590         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_381714, whole genome shotgun sequence.
ACCESSION   DATGEM010000590 DATGEM010000000
VERSION     DATGEM010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381714"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VKQ27_18080"
     CDS             complement(<1..800)
                     /locus_tag="VKQ27_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90897.1"
                     /translation="MAPNAKRVFYVKYLSGPVFGQILAKRPDIQLDKLENETPDAEAQ
                     PILSQAHAYQIGASRQVIGKHLQVTDALLAKTPNLVAVSSNGAGYDTIDVDSCTRNGI
                     IVVNQSGGNKEAVAEHALAMLLTLSKRIIEVDRRMRRGPGIPRNDYIGTELLDKTIGI
                     VGIGNVGGRLAELCKGLFRMRVLAYDPYLTREQITAKGAEKVATLDEMLRVADYVSIN
                     CPYTKETKDMVSARELRLMQKHAYLVTTARGGIHNEDALADALEKKQIA"
     gene            complement(816..2015)
                     /locus_tag="VKQ27_18085"
                     /pseudo
     CDS             complement(816..2015)
                     /locus_tag="VKQ27_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
     gene            2084..>2601
                     /locus_tag="VKQ27_18090"
     CDS             2084..>2601
                     /locus_tag="VKQ27_18090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY90898.1"
                     /translation="MSDRAGPPDVYVRAAPPPPPAQPLDGERNVDVAVIGGGFTGLST
                     ALHLSAAGIGVAVLEANEFGWGASRRAFGQVVPYLKLSEAAVLRHYGPERGPRVVEAV
                     AEGPDLVFELIRRHNIECWATRSGLIFGAHSAAGQRDLERRADYWRERTRAVEMLDAA
                     RCAELIGSTLYQA"
BASE COUNT          422 a          830 c          826 g          523 t
ORIGIN      
        1 gcgatctgct tcttctccag cgcatcggcc agcgcgtctt cattgtggat gccaccgcgc
       61 gccgtggtca ccagataggc atgcttctgc atcagccgga gctcgcgcgc gctgaccatg
      121 tccttcgtct ctttggtgta ggggcaattg atcgagacgt agtccgccac acgcagcatc
      181 tcatccagcg tcgccacctt ttccgccccc ttcgctgtga tctgttcgcg cgtcagatac
      241 gggtcatagg cgagcacccg catacggaac aggcctttgc acagctccgc aagccggccg
      301 ccgacgttgc cgatgccgac gatgccgatc gttttgtcca gcagttcggt gccgatgtag
      361 tcgttgcgcg gaatgccggg gccgcggcgc atgcggcgat ccacctcgat aatccgcttc
      421 gacagagtga gcagcatcgc cagcgcatgc tcggcgaccg cttccttgtt gccgcccgac
      481 tggttcacca cgatgatgcc gttgcgcgtg caggaatcga catcaatcgt gtcatagcca
      541 gcgccgttcg atgacaccgc caccaggttc ggcgtcttcg ccagcagcgc atcggtcact
      601 tgcaaatgct tgccgatcac ctggcgcgat gcgccgatct ggtaggcgtg ggcctgcgac
      661 aggatcggct gcgcctcagc gtcgggcgtc tcgttctcca gcttgtcgag ctggatatcc
      721 ggccgcttcg ccaggatctg gccgaagacc gggccggaca gatatttcac atagaagacg
      781 cgttttgcgt tgggtgccat tggacgtttt ccctttcagc tcaggctgcg gcagaagcgc
      841 cggatgcgtg cgcaggcttc ccgcagcgta tcggtgtcgg tggcatagct caggcggaaa
      901 tggtcggggc agaggaacac ggcgccgtgc accacggcca caccttcatc ctccagcaac
      961 gtagtgacaa atgtcaggtc atcgtcaaga cggacacctc gaggcgtggt cttgcccagg
     1021 cagcccgcca tcgaaggaaa caggtagaat gtgccttccg gcctgtggca ggtgatgccc
     1081 gggacatcgt ccagcatgtc cagcacgagg tcccgacgcg cgcggaaagc ccggaccatc
     1141 tcgctgatga aatcggttgg cccggtgagc gcggcaagcg ccgcggcctg tccgattgat
     1201 gacgtgttgg acgttgattg gctttgcagc ttgtccatcg ccctgatcag cggcaccggt
     1261 gcgccggcaa atcctatccg ccagccggtc atcgcatagg ccttcgacac accattcatc
     1321 gtgaccgtgc gatccttcag ccgaggctcc acctgcacga tggtcgccgg cacgtcgtcg
     1381 taggcgattt tctcatagat atcgtcggtc agcacccaga catcggggtg ccgcagcagc
     1441 acatcgcata gcgggcgcag atcgcgcgcc gtatatgcgg cgcccgtggg attgcacgga
     1501 ttgttcagga tgaaccagcg cgtacgagtc gagatcgcgg cttccaggtc ctcgccgcgc
     1561 agcttgaatc ccgcgttctg cccgcacggc acgaacaccg gtttgcctcc ggcgaccgat
     1621 acgatgtcgg gatagctcac ctagcacggc gtcggaatga tcgcctcgtc gctatcgttc
     1681 agcgtggcga gcattgcgtt gaacatcacc tgcttggcac cggtggagat gatcacttcc
     1741 tctggccggt actcgatgcc tgagtcacgg cggaatttgt ccgccaccgc acggcgcagt
     1801 tcgggcgtgc ctgcaacatc ggtgtatttc gtctcgcctg cctcgatcgc gcgagccgcc
     1861 gcatccttca cgtgctgggg ggtattgaag tcgggctcgc ctgaggacac gccgagcaca
     1921 tcgcgtccct cccgtttcag tgccgcgact ttctggcgca cggcgattgt ctggctgggg
     1981 gagatgcggt tcaggcgttc ggcggtgagc gtcatgcggc ggaaagtagc gtcctgttgc
     2041 gcatcgcggc aaccagccgc attttcgcgc aatgtatcag ccgttgtctg atcgggctgg
     2101 gccgcccgat gtctatgttc gtgcggcacc gccgcctccg cctgcgcagc cgctcgacgg
     2161 cgagcgcaat gtcgatgtgg cggtgatcgg gggcggcttc acaggcctgt cgaccgcctt
     2221 gcatctgtcg gcagccggta tcggcgtcgc ggtgctggag gcgaacgagt tcggctgggg
     2281 cgcatcgcgc cgcgccttcg gccaggtcgt gccttatctg aaactaagtg aggcggcagt
     2341 gcttcgccat tatgggcctg aacgcggtcc tcgcgttgtc gaggccgtgg ccgaaggccc
     2401 tgacctcgtg ttcgaactga tccgccgcca caacatcgaa tgttgggcga cgcgctccgg
     2461 cctgatcttc ggcgcgcatt ccgccgccgg ccagcgcgac ctggagcgcc gcgccgatta
     2521 ttggcgcgag cgcacgcggg cagtggagat gctggatgcc gcgcgatgcg ccgagttgat
     2581 cgggtcaaca ctgtatcagg c
//
LOCUS       DATGEM010000591         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_381840, whole genome shotgun sequence.
ACCESSION   DATGEM010000591 DATGEM010000000
VERSION     DATGEM010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381840"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1231)
                     /locus_tag="VKQ27_18095"
     CDS             complement(65..1231)
                     /locus_tag="VKQ27_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HLY90899.1"
                     /translation="MLHSHNRILTTHTGSLPRPPELTALYVRRSRGETIDLAELDTVG
                     KAAVREVVAKQIAAGIDIGNNGEQQREAFFLYVRHRMSGFGGGWTRKVFADVTHYPAF
                     SAWKAAHDAVNESISNTGGVPEAVAEVRYLDRALVEAECTDFRAALDGAHGGFAEPFM
                     TAPSPGIIAAAMRNAWYDTEDAYLAALGRALQVEYETIVGHGFLLQIDAPDLAMERHL
                     SYQDRSLSEFVDFVERVVTTINDALVNVPRDRVRLHACWGNYEGPHDCDVALPEILPA
                     IKRAKVGGFVLPFANPRHAHEYRCLNELPLDNDQVIVAGVIDPLTNFVEHPEVVADRI
                     EQVARVVGDPTRVQAGTDCGFDTSAGRGRVADDVVWAKLAAMAEGAKLATQRLF"
     gene            complement(1360..2355)
                     /locus_tag="VKQ27_18100"
     CDS             complement(1360..2355)
                     /locus_tag="VKQ27_18100"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HLY90900.1"
                     /translation="MHLARFPRVRLFPTPTPLEPLTNLSRELGGPEIWIKRDDCTVVA
                     TGGNKVRKLEWLIGEAQAQGATHIVTQGAVQSNHVRQTAAVARRFGMKCTALLEHRIE
                     TNDRDYLNSGNVLLDRLFDCAIEYRSAGADMNAEAETKGEALRAEGAKPYVIPGGGSN
                     RVGALGYVSCAQELVQQADEMGLTIDRLVTATGSAGTQAGLVVGLEGMNAGIPVLGIG
                     VRAPKDRQEANVHKLAEATAEYVGVRGGVARTAVAANCDYVGPGYGQPTPGMIEAVKM
                     LARLEGILLDPVYSGKAMAGLIDLVRKGEIRKGERVVFLHTGGAVGLFGYTRVFE"
BASE COUNT          441 a          911 c          756 g          487 t
ORIGIN      
        1 ccaaggccgg ctggcctcga ttccttgcaa atctggagca ggtcctggcg cgggtgggct
       61 cctcttagaa cagccgctgc gtcgccagtt tcgcgccttc tgccatcgcg gccagcttcg
      121 cccacacaac gtcgtcggcg acgcgaccgc gacccgccga ggtatcgaac ccgcagtcgg
      181 tgccggcctg gacacgtgtc ggatcgccta ccactcgagc cacctgctcg atgcgatctg
      241 ccacgacttc cggatgctcg acgaaattgg tcagtggatc gatgaccccc gccacgatca
      301 cctgatcgtt gtccaatggc agctcattca ggcaccgata ttcgtgcgcg tggcgaggat
      361 tggcgaacgg caacacgaaa cccccaacct tcgcccgctt gattgcgggg aggatctcgg
      421 gcagcgcgac atcgcagtcg tgcggccctt cgtaattgcc ccagcatgcg tggagccgca
      481 cccgatcgcg cggcacgttt accagcgcat cgttgatcgt cgtcaccacg cgctcaacga
      541 aatctacgaa ctcgctgagc gatcgatcct ggtaggaaag atgccgctcc atcgcgagat
      601 cgggcgcgtc gatctgcagc aggaacccgt ggccgacgat cgtctcgtac tccacctgca
      661 acgcgcgtcc aagcgccgcc aggtacgcat cctcggtgtc gtaccaggca ttccgcattg
      721 ccgcggcaat gatgccaggt gatggtgcgg tcatgaaggg ctcggcgaaa ccaccgtgcg
      781 cgccgtccag tgctgcgcga aagtctgtgc actccgcttc gaccagcgca cgatcgagat
      841 agcgcacctc ggcaacagct tccggcacgc cgccggtgtt gctgatcgac tcgttcaccg
      901 cgtcatgcgc cgccttccac gcgctgaacg ccgggtagtg agtcacatcc gcgaatacct
      961 tgcgcgtcca cccgccaccg aacccgctca tgcgatggcg cacatacagg aagaacgcct
     1021 cgcgctgctg ctcgccgttg ttgccgatgt cgatgcccgc ggcgatctgt ttcgccacga
     1081 cttcacgcac tgcagccttg cccactgtgt ccagctcggc cagatcgatc gtctcgccac
     1141 gcgaccggcg gacatacagc gcggtcagct ctggcgggcg tggcaggctg ccggtgtgcg
     1201 tggtgaggat acgattgtgg ctatgcagca tgtgagtgcc cttgtcattg cgagaggcgc
     1261 agcgtcgggc gccggattgt cattgcaatg gctagacgag attgcctcgt cactacgttc
     1321 ctcgcaatga ccaggggaga ggcctcagct ggtcatcgcc tattcaaaca cgcgggtgta
     1381 gccgaacaaa cccacggctc ccccggtgtg caggaacacc acacgctcgc ccttgcggat
     1441 ctcgcccttg cggaccaggt cgatcagccc tgccatcgcc ttccccgaat ataccgggtc
     1501 gagcagtata ccctccagcc gcgccagcat cttcaccgcc tcgatcatgc cgggcgtcgg
     1561 ctggccgtat cccggcccca catagtcgca gttcgccgcc acggcggtgc gagctacccc
     1621 accgcgtact ccgacatatt ctgcggttgc ctccgccagc ttgtgcacat tggcctcctg
     1681 gcgatccttc ggcgcgcgca cgccgatgcc cagcacaggg atgccggcat tcatgccttc
     1741 cagcccgacc accagccccg cctgcgtccc ggcgcttccc gttgccgtca ccagccggtc
     1801 gatcgtcagc cccatctcgt cggcctgctg cacaagttcc tgggcgcagg agacatagcc
     1861 aagcgctccc acccggttgg acccgccacc tgggatcacg tacggcttgg ccccctctgc
     1921 ccgcagcgcc tcgccctttg tttcggcctc cgcgttcata tcggccccgg ctgaccgata
     1981 ctcgatcgcg cagtcgaaca gccggtcaag cagcacgttg ccgctattca ggtagtcgcg
     2041 atcgttggtc tcgatccggt gctcgagcag tgcggtgcac ttcatgccga accgccgcgc
     2101 caccgcagcc gtttggcgca catggttcga ctgcactgcc ccttgcgtga cgatatgcgt
     2161 cgctccttgg gcctgcgcct cgccaatcag ccactccagc ttccgcacct tgttgccgcc
     2221 ggtcgccacc acggtgcagt cgtcccgctt gatccagatt tccggcccgc caagctcacg
     2281 gctcaggttc gtgagcggct ccaacggcgt cggggtcggg aaaagcctca cgcgaggaaa
     2341 gcgagcgaga tgcatgaatt ctcctaacaa tccggctgtg aggatgtaat gtcctccatc
     2401 ctcgcgcgtc ggacaatgca gtggggtcac tcaaggatga caaaatccgg agccccacca
     2461 gattgttcct gtgatacctt ggaggcaaag atgcacatca tgcgacgccg tggctgggaa
     2521 ctgtcggagc ggatggtcac ccccgaaccg ctggtgctga gtcgccgcgg ggcgctcgcc
     2581 ggtgctggtc tcctg
//
LOCUS       DATGEM010000592         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_220020, whole genome shotgun sequence.
ACCESSION   DATGEM010000592 DATGEM010000000
VERSION     DATGEM010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220020"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VKQ27_18105"
     CDS             <1..595
                     /locus_tag="VKQ27_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90901.1"
                     /translation="QMGRALQGSQLTEAPMQRIQPIAAKTDMAPEHQHVFEQVWDVFG
                     RVRGPFSMLLHSPKLAERLLPMVPFAREGMIVEPQLRQIAVLAMVREKDANYVWAAQV
                     DVARRVGLREAVIDLLRAKGDPAGLEPDERDIVVYARQLMRTNRVEQPVFDALLKRHG
                     AQWLVELTAVANFYVALCGVVNAFDVAVPEGGDRFTT"
     gene            complement(611..997)
                     /locus_tag="VKQ27_18110"
     CDS             complement(611..997)
                     /locus_tag="VKQ27_18110"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HLY90902.1"
                     /translation="MAIELTMLAWTAALCVVLAMPYTLGLIADRGLSTMAGNRDGLAP
                     ASGWISRAQRAHANLVENMAPFAALLLAVVVANRTSPTTAIAAQLFFYARAAHAVIYI
                     VGIPWLRSLAWGLGVVAMVMLFAALM"
     gene            1153..1515
                     /locus_tag="VKQ27_18115"
     CDS             1153..1515
                     /locus_tag="VKQ27_18115"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLY90903.1"
                     /translation="MQYILLIHSNEAAVKSAPKEATERMHAAYMAYTEALARAGVVVG
                     GNRLRPSSATTTVRLVDGKTQVLDGPYAETKEQIAGYYLIEVPDMDAALSWAARCPGA
                     NGGAIEVRPIWPNDASAG"
     gene            1496..>2467
                     /locus_tag="VKQ27_18120"
     CDS             1496..>2467
                     /locus_tag="VKQ27_18120"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLY90904.1"
                     /translation="MMRRRDESSAGWAAECAARHGYGKLVAFLAARTGNVTAAEDALA
                     DAFAAALADWPISGVPRKPQAWLMAVAKRRLIDAARRRRTRGDAADHMRLVAEELSED
                     VDADIPDRRLGLMFACAHPAIDAAMRAPLMLQTILGFDAAAIASAFLVSPATMGQRLV
                     RAKQKIKQAGIPLRVPDPEELPERLDAVLAAIYAAFAAEWSEIGNLADEAIWLGRLVV
                     ALLPEEAEALGLLALMLHVAARRDARRDAAGGYVPLAEQDTGLWDAGMIDEAETMLRR
                     ASGLGVIGRYQLEAAVQSVHAARRLTGRTDWVAIERLYAALTSITDSV"
BASE COUNT          430 a          718 c          883 g          436 t
ORIGIN      
        1 acaaatgggg cgcgccctac aaggcagtca actgacggag gcacccatgc agcgcatcca
       61 gccaatcgcc gccaagaccg acatggcacc tgagcaccag cacgtgttcg aacaggtgtg
      121 ggacgtgttc ggccgtgtgc ggggcccctt cagcatgttg ctgcacagtc cgaagctggc
      181 cgagcgtctg ttgcccatgg tgccgttcgc gcgcgagggc atgatcgtcg aaccacagct
      241 gcgccagatc gctgtactcg caatggtccg cgagaaggac gccaactatg tctgggctgc
      301 acaagtcgat gtcgcgcgcc gtgttggctt gcgtgaagcg gtcattgatc tgctgcgtgc
      361 caaaggcgat ccggccgggt tggagccaga cgaacgcgac atcgtcgtct atgcgcgcca
      421 gttgatgcga acgaatcgtg tcgagcagcc ggtgttcgat gcgctgctga agcgtcatgg
      481 cgcgcaatgg ctggtcgagc tgacggcggt cgccaacttc tatgtcgcgc tgtgcggcgt
      541 ggtgaacgcg ttcgatgtgg cggtgccgga gggtggggat cggttcacga cgtagattct
      601 gcgctgggag tcacatcaat gcggcaaaca gcatgaccat tgcgaccacg cccagtcccc
      661 aggcaagcga gcgcagccag ggtatgccga cgatatagat cactgcgtgt gccgcgcgcg
      721 catagaagaa caactgcgcg gcgatggcgg ttgtcgggct cgtccggttc gcgacgacaa
      781 ccgcgagcag caaggcggcg aaaggcgcca tgttctctac caggttggca tgcgcccgtt
      841 gtgcgcggct gatccagccg gaggcaggcg cgagcccatc gcggttgccg gccattgtgc
      901 tcagaccgcg atcggcaatc aggccaagtg tgtacggcat ggccagcacg acgcacagcg
      961 cggcagtcca tgccagcatg gtcagttcaa tcgccacgat gtttccctct acaggtgcga
     1021 cacatggacc cttcgcccgg ctgtccgccg cgatcaattc gacatgactc ggcgcctgtc
     1081 gaaatccggg gaactcgaac gtcattgggt tgtccgtgca tcatgcgcgg cgatccagtg
     1141 caggagaccg agatgcaata catattgctg atccactcga acgaggcggc ggtgaagtcg
     1201 gcaccgaagg aagcgaccga acgcatgcat gctgcctaca tggcgtatac cgaggcgctt
     1261 gcgcgggctg gcgtggtcgt cggtggcaac cggctgcggc catccagcgc gacgacgacg
     1321 gtacgtctgg tcgatggcaa gacgcaggtg ctcgacggcc cgtatgccga gacgaaagaa
     1381 cagatcgcag gctactacct gatcgaggtt cccgacatgg acgctgccct gtcatgggcc
     1441 gcccgttgtc ccggcgccaa tggcggtgcc atcgaggtac ggccgatctg gccgaatgat
     1501 gcgtcggcgg gatgagtcgt cagccggatg ggccgccgaa tgtgcggccc gtcacggtta
     1561 cggcaagctg gtcgcgtttc tcgcggcgcg caccggcaac gtgacggcgg ccgaggatgc
     1621 gctggcggat gccttcgcgg ctgccctcgc ggactggccg atcagcggcg tgccgcggaa
     1681 accccaggcc tggctgatgg ccgtcgcgaa gcggcggctg atcgacgcgg cgcgccggcg
     1741 gcgcacgcgc ggtgacgcgg ccgatcatat gcgcctggtg gccgaggagc tcagtgaaga
     1801 cgtggacgcg gatattcccg atcgtcggct ggggctgatg ttcgcctgtg cacatccggc
     1861 gatcgacgcg gcgatgcgcg caccgctgat gctgcagact atcctgggtt tcgatgccgc
     1921 ggcgatcgcc tcagcgtttc tggtgtcacc cgcgacgatg gggcagcggc tggtgcgggc
     1981 gaagcagaag atcaagcagg cgggcattcc tctgcgtgtt ccggacccag aggagctgcc
     2041 ggagcggctg gatgctgtat tggcagccat atatgcagcc ttcgcggcag agtggtcgga
     2101 gatcggcaat ctggcggatg aggcgatctg gctgggcagg ctggtcgtcg cgctgttgcc
     2161 ggaggaggcg gaggcgctcg gtctattggc gctgatgctg cacgtggcgg cgcgtcgcga
     2221 tgcgcggcgt gatgcggctg gcggttatgt acctctggca gagcaggata ccgggctgtg
     2281 ggatgccgga atgatcgatg aagccgagac catgctgcgg cgtgcgagtg ggttgggcgt
     2341 gatcgggcgg taccagctcg aagcggctgt gcagtctgtg catgcggctc ggcggttgac
     2401 cgggcggacg gactgggtag cgatcgagcg gctttatgcg gcgctgacat cgatcacaga
     2461 ttcggta
//
LOCUS       DATGEM010000593         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_498078, whole genome shotgun sequence.
ACCESSION   DATGEM010000593 DATGEM010000000
VERSION     DATGEM010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498078"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VKQ27_18125"
     CDS             complement(<1..1669)
                     /locus_tag="VKQ27_18125"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLY90905.1"
                     /translation="MLNRLIVGMADFSQRRALAVTLGGLLFAILASLFAVSHLGVSTD
                     TDLMFSPNLPWRQQAEELNKDFPQFNNLLVAVVDAKEPEEADATAAALADAVAKDHAH
                     FNNVRRPDASPFLHREGLLFLDTKQLSDLMDRTIDAQPFLGQLVADPTARGLFSALSL
                     LGMGVQKGDVDLSPYLASLRAFNQSMVDALGGHPQPLSWQLLLGGSRLNDLAGKYKFV
                     LVQPKLDYGALQPGGEATEAMRQIIANLEYVKAGDARVRITGQIALADEEFATVVQGM
                     VWGLIGSLTLITLWLFLAVKTWRLIVPILVTLSMGLVCTLLFAAIAVGTLNLVSVGFG
                     ILFVGIAVDFAIQFSVRYRERRFEYPDPAEAMRQNAHRTGVQILVAACATAAGFLGFV
                     PTDFSGVAELGLIAGVGMLIAFVCTLGFLPAVITLCRPRGERAVVGFAGLAPLDPLIA
                     RHRRPILIVFGVLAVVAVGLSPRLQFDSDPLHTKNPDTEAMRTLYDLMNDPVTNPYSI
                     SILEPNVADAQALKAKLVKLPTVSKVINVDSFVPDDQQAKLAIIADAN"
     gene            1819..2964
                     /locus_tag="VKQ27_18130"
     CDS             1819..2964
                     /locus_tag="VKQ27_18130"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HLY90906.1"
                     /translation="MSSSTMTQSQVGPRNIPPFRADHVGSLLRPRELAAARAQRKAGA
                     LSAEELRAVEDRCIEAAIRKQEEIGLRAATDGEYRRAFWHYDFVSGLDGIELYAPEQK
                     ILFKGGLPLPLMLRVVGKIGWSKPVMVDDFRFVASHVKTAMPKMTIPSPSVAHFRGGR
                     QAIDAKVYPEMDAFFADLGEAYHAAVAAFGAAGCHYLQLDEVNIAYLCDPEQIAGLKA
                     RGEHVENLLSIYAGMLNRAIEAKPAGMTISMHLCRGNFQSHWVASGGYEPVAEALFNQ
                     INSDAYFMEYDSERAGGFEPLRFVPRGHKVVVLGLVTSKTGALETKDELKRRIDEAAK
                     YLPLEQLALSPQCGFASTEEGNTLSEDEQWAKLRLCVEVAREVWGGV"
     gene            2991..4475
                     /locus_tag="VKQ27_18135"
     CDS             2991..4475
                     /locus_tag="VKQ27_18135"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY90907.1"
                     /translation="MDTDEVHGAKRIGVHPGPSVVFNSCLWLILVATVLRLGFGAALG
                     LGVDESYMVASGRVMSLGYFDHPPAAWWLSWGAAHLFGSEAPVIVRLPFIGLFALSTW
                     LMYRLGGFWAALLLNLSPVFGVTTGTWVLPDGPLDCALLAATLCLMRALERGTLGWWL
                     GAGLCAGLALFSKYSAVLTLAGAGGFLLTSPQHRRLLAGSKPWLGLVVAALVFSPVLV
                     WNAQHGWASFAFQGGRAEGLRFHPFAPLATLGGEALFVLPWIWAPMMVLWIARARRGP
                     AEWRSWLLCWLAAPPILVFALIAAWSSQRVLFHWAAPGYLMLFPLLGHAVAQRIGQPP
                     VPRLIWGTAGFVVLAVLVVATQVRFDWLPLAQFARRDPDIEAVDWTSLRAELALKPGA
                     LVGVPNWRDAGKVAYALGPDVTTICLNRDARQFGYAVPAGHFVGRDMLILAPEHGERV
                     PQELGSVFEAMEPLAPVPVRHAGHTLFSVAVFRASHLRAWPPPG"
     gene            4504..5403
                     /locus_tag="VKQ27_18140"
     CDS             4504..5403
                     /locus_tag="VKQ27_18140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLY90908.1"
                     /translation="MNSVLTSAELRSLSGRSNAEGALRTGIHVVLLASSCWLLAISGA
                     WTLLPALIAFGLVQVALFAPAHEATHQTAFASRRANHVVGWLASCPSLLNLEFYTAFH
                     FAHHRHTQIPGRDPELDMAAPHDVASYLRRVLGIPFWRLRLLVVADAWRGDMSRYPYV
                     SAQAAPVIIRSVRAMSATMVGGSIVAVLVFGWVAPLKFWILPQLLCQPFLRAYVLAEH
                     TGCTEDRNGLTNTRTTLTNAVVRLLMWNMPYHAEHHLYPSIPFHRLADAHMMLRAKLG
                     FIEPCGYVRWNASVIRSLAGNAR"
     gene            complement(5411..>5594)
                     /locus_tag="VKQ27_18145"
     CDS             complement(5411..>5594)
                     /locus_tag="VKQ27_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90909.1"
                     /translation="GIIFDIAAHGWAGSQKNPGDADNIVHPNPQRRLAKFDERRARAQ
                     AKIASLRAKEVAAAAE"
BASE COUNT          893 a         1649 c         1976 g         1076 t
ORIGIN      
        1 aattggcgtc ggcgataatc gccaacttcg cctgctgatc gtccggcacg aagctgtcca
       61 cgttgatcac tttggacact gtgggaagct tcaccagctt cgccttcaga gcctgggcgt
      121 ccgccacgtt cggttccaga atgctgatgc tgtacggatt ggtgaccgga tcgttcatca
      181 ggtcgtacag cgtgcgcatc gcctcggtgt cggggttctt tgtgtgcagt ggatcagagt
      241 cgaactgcag gcgcggcgac aggccgacgg ccaccacggc gaggacgccg aacacgatca
      301 ggatcggccg ccgatggcgc gcgatcagcg gatcaagcgg ggccaggccg gcgaaaccca
      361 ccaccgcccg ttcgccgcgt ggccggcaca acgtgatcac ggcgggcagg aagcccagag
      421 tgcagacgaa ggcgatcagc atgccgacgc ccgcgatcag gccgagttct gccacgccgg
      481 agaaatccgt tggcacgaag ccgagaaagc ctgccgccgt ggcgcaggcc gcgaccagga
      541 tctggacgcc ggtgcgatgc gcgttctgcc gcatcgcctc tgctggatcg ggatactcaa
      601 agcggcgttc gcggtatcgg actgagaact ggatagcaaa atccactgcg atgccgacga
      661 acaggatgcc aaatcccacc gagaccaggt tcagcgtgcc gacggcgatg gcagcgaaca
      721 gcaacgtgca gaccaggccc atgctgagcg tcaccaggat tggcacgatc agccgccagg
      781 ttttcaccgc caggaacagc cacagcgtga tcagcgtcag gctgccgatg aggccccaga
      841 ccatgccctg gacgaccgtg gcgaattcct cgtcggccag tgcgatctga ccggtgatac
      901 ggacacgcgc gtcaccggct ttcacatact ccagattggc gatgatctgg cgcattgcct
      961 cggtggcctc gccgccgggc tgcagcgcgc cgtaatcgag cttgggttga accagcacga
     1021 atttgtactt gccggccaga tcattcaggc ggctgcctcc cagcagcaac tgccaggaaa
     1081 gcggttgcgg gtgacccccc agggcatcaa ccattgattg gttgaaggcg cgcagcgacg
     1141 cgaggtaggg gctcaggtcg acgtcgccct tctgcacgcc catgccgagc agcgacagag
     1201 cagagaacaa gccgcgtgcc gtggggtccg cgaccagttg gcccaggaac ggctgagcgt
     1261 cgattgtgcg gtccatcaag tcgctgagct gcttggtgtc caggaagagc aggccttccc
     1321 ggtgcaggaa cggcgaagcg tccggccggc gcacgttatt gaaatgggcg tgatccttgg
     1381 cgaccgcgtc cgccagggcc gcggcggtcg catcggcctc ttccggctcc ttcgcgtcca
     1441 ccactgccac cagcaggtta ttgaactgcg ggaaatcctt gttgagctcc tcggcctgct
     1501 gacgccaagg caggttgggc gagaacatca ggtcggtgtc ggtgctcacg ccaaggtgcg
     1561 acaccgcgaa gagcgaggcg aggatggcga acagcaggcc gcccagcgtc actgcgagcg
     1621 cgcggcgctg gctgaaatcg gccattccaa cgatcaggcg gttcagcatg ctccgggacg
     1681 cgacagtggg gcggcccggc ggcagcgggc tgcgtgtgat aagcgtttgg gcggcctcgg
     1741 tccatggtgc atccgggtgg ttttcaccag aacggtcagg cggtaggaga ggcggctgat
     1801 ggtcgggagg ttggtccaat gtcctcatcc acgatgacgc aaagtcaggt cgggccgcgc
     1861 aacataccgc cgttccgtgc ggaccatgtg ggcagcctgc tgcgtccacg cgagttggct
     1921 gctgcccgcg cccagcgcaa ggccggggcg ctgtccgcgg aggagctgcg ggctgtcgag
     1981 gaccgctgca tcgaagcggc gatccgcaag caggaggaaa tcggcctgcg tgcggcgacc
     2041 gatggcgagt atcggcgtgc cttctggcat tacgattttg tctcgggcct tgatggcatc
     2101 gagctgtatg cgcctgagca gaagatcctg ttcaagggtg gcctgccgct gccgctgatg
     2161 ctgcgcgtgg tgggcaagat tggctggagt aagccggtga tggtggacga ttttcgcttc
     2221 gtcgccagcc atgtgaagac ggcgatgcca aagatgacca ttccgtcgcc gtccgtcgcg
     2281 cattttcggg gcgggcgtca ggcgatcgac gcgaaggtgt atccggagat ggatgcgttc
     2341 ttcgctgatc tcggggaagc ataccatgcg gcagtggcgg cgtttggcgc ggccggatgc
     2401 cactacctgc aactggacga ggtgaacatc gcctatctat gcgatcccga gcagatcgct
     2461 ggattgaagg cgcgcggcga acatgtggag aacctgctgt cgatctatgc ggggatgctg
     2521 aatcgcgcga tcgaggcgaa gccggctggc atgacaatca gcatgcatct ttgcagggga
     2581 aattttcagt cccattgggt ggcgtcgggt ggctacgagc cggtggccga ggcgctgttc
     2641 aaccagatca actccgatgc gtatttcatg gagtacgata gcgaacgggc gggtgggttc
     2701 gagccgcttc ggtttgtgcc tcgggggcac aaggtggtgg tgcttgggct ggttaccagc
     2761 aagacggggg cgcttgagac gaaggacgag ctgaagcggc ggatcgatga ggccgcgaag
     2821 tacttgcccc tggagcagct cgcgctgtcg ccgcaatgcg ggtttgccag caccgaagag
     2881 ggcaacacgc tgtccgagga cgagcagtgg gcgaaactgc ggctatgcgt cgaagtggcg
     2941 cgcgaggtgt ggggcggggt gtaagaaaat ggaaccacag atggacacag atggacacag
     3001 atgaggttca tggcgcgaag cgcatcggtg tccatccggg tccatctgtg gttttcaatt
     3061 cttgcctttg gctcattctc gtcgcgacgg tgctgcggct gggcttcggt gcggcgcttg
     3121 ggctcggagt cgacgaaagc tacatggtcg catcggggcg tgtgatgagc ctcggctact
     3181 tcgatcaccc tcccgcggcg tggtggctga gctggggcgc ggcacacctg ttcggctccg
     3241 aagcaccggt catcgttcgg ctgccgttca ttgggctgtt cgcgctgtcg acctggctga
     3301 tgtaccggct tggcgggttc tgggcggcgc tgctgctgaa cctttctcca gtgttcgggg
     3361 tgacgacggg gacctgggtg ctgccggacg ggccgctcga ttgtgcgctg ttggcggcca
     3421 cgctctgtct gatgcgcgcg ttggagcggg gaacattggg atggtggctt ggcgccgggc
     3481 tgtgtgccgg gctggcattg ttttcaaagt attcggccgt gctgacgctg gctggtgccg
     3541 gtgggttcct actgaccagc ccccaacacc ggcggctact ggccggttcg aagccttggc
     3601 ttggactggt ggtcgcagcc ctggtattct cgccggtgct ggtctggaac gcgcagcatg
     3661 gctgggcgtc gtttgcattc cagggcggtc gggctgaggg actgcggttc caccccttcg
     3721 cgccactggc cacgctgggc ggcgaggcgc tgttcgtgtt gccgtggatc tgggcgccga
     3781 tgatggtgct ttggatcgcg cgggcgcggc gggggccggc ggagtggcgg tcgtggctgc
     3841 tgtgctggct ggcggcgccg ccgatcttgg tgtttgccct gatcgcggcc tggtcgagcc
     3901 agcgggtgtt gtttcattgg gcagcgcccg gctatctgat gctgttcccg ctgctgggac
     3961 acgccgtcgc gcagcgtatc ggtcaaccgc cggtacccag gctgatttgg ggcaccgcgg
     4021 gttttgtggt gctggcggtg ctggttgtgg cgacgcaagt gcggttcgac tggctgccgc
     4081 tggcgcagtt tgcgcggcgc gatccggata tcgaagcagt ggactggaca tcgctgcgtg
     4141 ccgagctggc gctgaaaccg ggggcgttgg tcggggtgcc gaattggcgg gatgccggga
     4201 aagtcgccta tgcgctgggg ccggacgtca cgacgatctg cctgaaccgg gacgcccgcc
     4261 aattcggcta cgccgttccg gctgggcact tcgtcggccg tgacatgttg atcctggcgc
     4321 ctgagcacgg cgagcgtgtg ccgcaggaac tcgggagtgt gttcgaagcg atggaacctc
     4381 tggcgccagt gcccgtccgc catgccgggc acacgctgtt cagcgttgcg gtgttccgtg
     4441 ccagccatct gcgtgcctgg ccgccgcctg gataatggca caggccttgc ttacgatccc
     4501 ggcatgaaca gcgtgctgac gtcagcagaa ttgcgctcgc tctctggccg cagcaatgcc
     4561 gagggcgcgc tgcgaactgg tatccatgtg gtcttgctgg ctagcagctg ctggctgctg
     4621 gcgatttccg gcgcgtggac gctgctgccg gcgctgatcg cgttcggcct cgtgcaggtg
     4681 gcgttgtttg cgccggcaca cgaggcgact caccagacgg ccttcgcgtc gcggcgggcg
     4741 aaccatgttg tcggctggct ggcctcctgc ccgtcgctcc tgaacctgga gttctacacc
     4801 gcgttccact tcgcccatca ccggcatacg caaattcccg ggcgggaccc ggagctggac
     4861 atggcagccc cgcatgacgt cgccagctat ttgcggcgtg tcctgggcat tccgttctgg
     4921 cgtctgcgcc tgctggtggt ggcggatgcc tggcggggtg acatgagccg gtatccgtat
     4981 gtctctgcgc aggcagcgcc cgtgattatc cgcagcgtgc gggcgatgag cgccacaatg
     5041 gtgggtggct ccatcgtcgc ggtgctggtg tttggctggg tggcaccgct gaaattctgg
     5101 attttgccgc agttgctgtg ccagccgttc ctgcgcgctt atgtgctggc tgagcacacc
     5161 ggctgcacgg aggaccgcaa cggcctgacc aacacacgca ccacgctgac caacgccgtg
     5221 gtgcggctgc tgatgtggaa catgccgtac cacgccgagc accaccttta cccctcgatc
     5281 ccgttccacc ggctggccga cgcacacatg atgctgcgcg ccaagctcgg cttcatcgag
     5341 ccgtgcggtt acgtgcggtg gaacgccagc gtgatccgat cgctggcggg gaatgctagg
     5401 taaccggcgg ttactcggcg gcggcggcaa cttccttcgc gcgcaacgac gcgatcttcg
     5461 cctgggcgcg ggcacggcgc tcatcgaatt tggcgagacg gcgctgtggg ttcggatgca
     5521 cgatgttgtc ggcatcgccc gggttcttct gcgagccagc ccaaccgtgt gcggcgatgt
     5581 cgaagatgat gccg
//
LOCUS       DATGEM010000594         9324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1322008, whole genome shotgun sequence.
ACCESSION   DATGEM010000594 DATGEM010000000
VERSION     DATGEM010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9324
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322008"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VKQ27_18150"
     CDS             complement(<1..292)
                     /locus_tag="VKQ27_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLY90910.1"
                     /translation="MMKLHWSPRSPYVRKVMVVAHELGLADRIETVRTVVGGTTPHLE
                     LMRENPLGKIPTLVLEDGTILYDSPVICEYLDTLHAGEKLFPAGSENWTARRR"
     gene            346..1137
                     /locus_tag="VKQ27_18155"
     CDS             346..1137
                     /locus_tag="VKQ27_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY90911.1"
                     /translation="MNWIEVNGTGLRYDISGAGKTTLVLVHEMGGTLDSWDQVVPALN
                     HARRVLRYDTRGAGISEKIGGKVTFDDMADDLAALLDATGITGKIAIAGTAVGGAIAI
                     HFAVRHAARAGALAVTSPATGVPAERRQATLDRAAAVEAKGMRGIVDGSVANSYPPEV
                     RHNADDFRKFRARWLGNDPQSFAAINRMLADSTVTEELPRIACPTLVIGCTHDKLRPP
                     SDVEPMAKQIPGAEYMTINSGHFAGIQTPGLVSQAFHGFFSAHGY"
     gene            1189..2928
                     /locus_tag="VKQ27_18160"
     CDS             1189..2928
                     /locus_tag="VKQ27_18160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLY90912.1"
                     /translation="MAAAAIVIPLFHRLRLSPVLGFILVGIAVGPFGFAMFADKVPLL
                     GAIMLNQHELIASIAELGISMMMFMIGLELSWERLWVMRRLVFGFGLLQFALCAIAIA
                     AVVLVLGASPLLAVIAGTALAMSSTAVVVQVLSQEKRLGAPVGRASLAVLLLQDIAAV
                     PVLFVISVSGAESSASWTIVQAVAVVIGLIVGGRLVLRPLFRSVARTGSPELFVAACL
                     LVILSTSLAAAVVGLPMELGSLIAGLLLAETEFRRQIEITIDPFRGLLLGVFLISVGM
                     SLDLASIVNDLPRIVLAGFVLLVVKAAIIACLARMFGLHWATGWQAGLLLGPGGEFGF
                     VIIGLARTAGLIDADKTRFPLVLIAATMAAIPLLSTLGRRVTHRASTRPMIDPALLAL
                     PPGDATPRVIVAGLGRVGETVAALLERHRVAYIAVDGDTDRVAQQRKLGRPVYWGDIT
                     QTELLHRLHLETARALVVTLGDHDASDRLVAATRTARPDLLIIARARDAPHAAHLYAM
                     GVSDAVPETIEASLQLAEAVLVEIGLPMGPVIATIHERRAELQAEIKAMAPHAEVRVR
                     GHRRLRDMLPRAR"
     gene            complement(2984..3697)
                     /locus_tag="VKQ27_18165"
     CDS             complement(2984..3697)
                     /locus_tag="VKQ27_18165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY90913.1"
                     /translation="MLEAADVQPGDQVLDMCTGTGEAAVAIASAIGPSGRLIGADIAP
                     PMLECARDRLNEPTFLPIAADGQALPFADSCFDAVVCQLGLQFFPDPGLGLKEFRRVL
                     RDGGRAGICVISSPDRAPMWGILAGLLGRLLPAQQQTVHLSFSLCDPARLETLLINAG
                     FQQVLIERQTREDSYGSFDEYWDSIEAGIGSIPQTYLMLPDAARLEVRQTMRAKLAEF
                     EADRRLRMSLEMLIGSGVA"
     gene            complement(3874..4551)
                     /locus_tag="VKQ27_18170"
     CDS             complement(3874..4551)
                     /locus_tag="VKQ27_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HLY90914.1"
                     /translation="MSGTQKGINRQQMADRIAFEFQDGWIVNLGVGIPTMCSNTDIGD
                     RDITYHSENGVIGYGPIAPAGEEDLNLVNAGGQNVTLKAGAAVVHHADSFAIIRGGRI
                     DVTVMGAYEVACNGDFANWKISGQKGGGIGGAMDLAACAKRVFIIMEQATRSGAPRLV
                     AQCALPVTARGVVKLVATNYGLFEPIGDGFRIREIAPGVTLDEVHASVGCKLTVPENL
                     PTMRVVS"
     gene            complement(4548..5288)
                     /locus_tag="VKQ27_18175"
     CDS             complement(4548..5288)
                     /locus_tag="VKQ27_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HLY90915.1"
                     /translation="MTQKSKQVATFRDAVADIPDGATIAFGGFAMPGTPFNLIRALLE
                     HGAKNLTLVGNTTGGAQQPRMPDMGMLVENGQVKKVICAFTAATRPTDVLPFTKYYES
                     GEVDAELVPQGTLAERLRAAGAGIPAFYTPTAVGTELAQGRETRVIGGREYLLEYALP
                     LDYAFVRATRADTFGNLQFHRSQRNFCPVMATAARVTIAEVDEDILPAGAIDPDQVHT
                     SGIFVTRVVKVPPHPEGLWPQRRQERRR"
     gene            complement(5996..7579)
                     /locus_tag="VKQ27_18180"
     CDS             complement(5996..7579)
                     /locus_tag="VKQ27_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY90916.1"
                     /translation="MKRRDFLAGSAALTLAAPAVRAAGDTKLLKFIPQADIALLDPHF
                     ASALVTRNHAYMVFDTLYGVDDAARPRPQMAAGHVVEDDGKTWKITLREGLRFHDGTP
                     VLARDAVASLKRWGKRDVFAISVFSQVDDLSAISDTVLQFRLKRPFRLLPDLLAKPTP
                     YASAIMPERLAANAPAQQVTELIGSGPYKFVMKERVPGSLAVYARNTDYVPRTDGPPV
                     GTAGPKIAYFDRVEWHTIPDPATAAGALQSGEMDWWEQPTTDLLPLLRKNPDVKVDII
                     DTLGYQAVLRFNHLYPPFDNAGIRRALLGAFSQTDMMQAVAGEDPSMWRAGVGIFSPL
                     SQFANDAGMDVLNSPRDLGKVKRDLAAAGYNSEKIVFLVPTDLPAINAMSEVAAEVFH
                     KLDMNLDYQALDWATVAQRLLSQAPPDKGGWSLNANYAPGFAAMSPAAHSFLRGLGKQ
                     SLFGWPTEPRVEELRSAWIDATDLEEQKRLCREIQLQAFQDVPYIPLGAFFFAGAYRK
                     NLTGILKGGVAQFTNVRRM"
     gene            7710..8552
                     /locus_tag="VKQ27_18185"
     CDS             7710..8552
                     /locus_tag="VKQ27_18185"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY90917.1"
                     /translation="MNSKPQLRPLGEALGTEALGIDLSEPLDDATFDWVANAFAEHPV
                     LVFRDQDIGAAPLAEFGRRFGAPRRHALIKYRHADCPEVSWLTNVDEEGKVDWYGVKR
                     ATDWHTDSTYEAELPLLAILHAKEVPSEKGGTMFADMRAAYDALPDARKQQLEGLTAL
                     HGRSVGPAGEKLYGADKGITVKLPENVQWPAVTRHPVTGRQVLFVNPMHTYGFAGMTQ
                     DEAWPLIEELAEHATQDRFVYYHRWRVGDVLMWDERATMHRGAGDYRPEERRIMLRTI
                     VYPG"
     gene            complement(8651..>9324)
                     /locus_tag="VKQ27_18190"
     CDS             complement(8651..>9324)
                     /locus_tag="VKQ27_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90918.1"
                     /translation="ERWLADHLIVYNASRATGGANPFNQNLGTVDTELPVQEFDLRKI
                     GSMQTEAGGSAKEYELRLLRETDNKPDGSAGTRKVTGSTHAIRAYFLPWGGGRTYCGK
                     LGINAEYFFTPTLNGCTFGWRGHGPSPSVAHSNFANPVTQQADQNAMDTDMTTKFGGS
                     LPAHMLVKTDYKRAPIGLEDYRSTVVGIRAGNTWHFYYQNYKAESLGGGKVTKTGIGL
                     CIPIV"
BASE COUNT         1619 a         2934 c         3022 g         1749 t
ORIGIN      
        1 gccgccgccg tgctgtccag ttctcgctgc ccgcgggaaa cagtttctcg ccggcatgca
       61 acgtatcgag atactcacag atcaccggcg aatcgtacag gatcgtcccg tcttccagca
      121 ccagcgtcgg aatcttcccc agcgggttct cccgcatcag ctccagatgc ggagtcgtcc
      181 ctccaaccac cgtgcgcacc gtttcgatcc gatccgccag cccgagctca tgcgcgacca
      241 ccattacctt gcgcacatag ggcgagcgcg gcgaccagtg cagcttcatc atgccatgtc
      301 ctccatcgcc gcagtgtggc atcctggcgt tgaggaggaa atgcgatgaa ctggatcgaa
      361 gtgaacggca ccggcctgcg ctacgacatc tctggtgctg gcaagaccac gctggtgctg
      421 gtgcacgaaa tgggcggcac gctggacagt tgggaccagg tggtgcccgc gctgaaccac
      481 gcgcgccgcg tgctgcgcta tgatacgcgc ggcgccggca tttccgagaa gatcggcgga
      541 aaggtcacct tcgacgacat ggcggacgat ctcgcggcct tgctggatgc gaccggcatc
      601 accggcaaga ttgcgattgc cggcactgcg gtaggcggcg ccatcgcgat ccactttgct
      661 gtgcgtcatg cggcccgtgc aggcgcgctg gccgtcacca gcccggccac cggtgtcccg
      721 gcagaacgcc ggcaggccac actggatcgc gccgccgctg ttgaagcaaa aggcatgcgc
      781 ggcatcgtcg atggcagcgt cgcgaactcc tacccgcccg aggtgcgcca caacgcggat
      841 gacttccgca agttccgtgc acgctggctc ggcaacgatc cgcaaagctt cgccgctatc
      901 aatcgcatgc tcgcggattc aacggtcacc gaggagctgc cgcgcatcgc ctgtcccacg
      961 ctggtgattg gctgcacgca tgacaagctg cgcccgccat ccgacgttga gccgatggcg
     1021 aaacagatcc ccggcgccga gtacatgaca ataaactcgg ggcatttcgc cggtatccag
     1081 acgccgggcc tggtgtcaca ggcctttcat gggttcttct ccgcgcacgg gtactgacgc
     1141 ctgggcgctc acgtcgatcc agggctgttc aagccggccc tgatcatcct ggccgccgcg
     1201 gccattgtga tcccgctgtt ccatcggctg cggctgagcc cggtgctcgg cttcatcctc
     1261 gtcggcatcg ccgtcggccc gttcggcttc gcgatgttcg ccgacaaggt gccgctgctc
     1321 ggcgccatca tgctgaacca gcacgagctg atcgcatcga tcgccgaact cggcatcagc
     1381 atgatgatgt tcatgatcgg gctggagctg tcatgggagc ggctctgggt catgcgccgg
     1441 ctggtattcg gcttcggcct gttgcagttc gcgctatgcg ccatcgctat tgccgcagtc
     1501 gttctcgtgc tcggcgcatc accgctgctc gcggtcattg caggcacggc gctcgcgatg
     1561 tcgtccaccg ccgtcgtcgt gcaggtgctg tctcaggaga agcgtctggg cgcaccggtt
     1621 ggccgcgcat cgctcgcggt cctgctgttg caggacatcg ccgccgtgcc ggtgctcttc
     1681 gtcatcagcg tatccggtgc ggagagcagc gcctcctgga ccatcgtgca agcggtggca
     1741 gtggtcattg gcctgatcgt cggcggccgt ctggtgctgc gaccattgtt ccgcagcgtg
     1801 gcgcgcactg gcagtcccga gctgttcgtc gccgcgtgcc tgctggtgat cctgtccacc
     1861 agcctcgccg cggcagttgt tggactgccg atggagctgg gctcgctgat cgcgggactg
     1921 ctgctggcag aaaccgagtt ccgccggcag atcgagatca ccattgatcc attccgaggg
     1981 ctgctgcttg gcgtgttcct gatttccgtc ggcatgagcc tggaccttgc cagcatcgtc
     2041 aacgatttgc cgcgaattgt gctggcgggc ttcgttctgc tggtggtgaa ggccgcgatc
     2101 atcgcctgtc tcgcgcgcat gttcggcttg cactgggcga cggggtggca agccggattg
     2161 ctgctgggcc cgggtggtga gttcggcttc gtgatcatcg gcctggcgcg cactgcaggc
     2221 ctgatcgatg ccgacaagac gcgctttccg ctggttctta tcgcggcgac gatggccgcg
     2281 atcccgcttc tctccacgct gggtcgccgg gtaacgcacc gcgcgtcaac gcggccgatg
     2341 atcgacccgg cgctcttggc actgccaccc ggcgacgcca cgcctcgcgt catcgttgcg
     2401 ggcttgggac gtgtcggcga aacggtcgcg gcgctgctgg aacggcatcg cgttgcctac
     2461 atcgccgtcg atggggatac cgatcgcgtg gcgcagcagc gcaagctggg gcggccggtc
     2521 tactggggcg acatcacgca gactgagctg ctgcaccgcc tgcacctgga aaccgcgcgc
     2581 gccctggtgg tgaccctcgg cgaccacgac gccagtgatc gcctcgtggc tgccacccgc
     2641 acagcccgcc cggatctgct gatcatcgcg cgcgcccgcg atgcacccca tgcggctcac
     2701 ctttacgcta tgggcgtgag cgatgcggtg cccgaaacga tcgaagcgag tctgcagctt
     2761 gccgaagccg tgctggtgga gatcggcctt ccgatgggac cggtgatcgc gactatccac
     2821 gaacggcgcg cggaattgca agccgagatc aaggcaatgg cgccgcacgc cgaggtgcgt
     2881 gtgcgaggcc atcgtcgcct gcgcgacatg ttgcctcgcg cacggtgatc gcccagttgg
     2941 agcggtgcac atcggcgtca caccggccgg accaagcggt ggttcaggcg acgccactgc
     3001 cgatcagcat ttcaagactc atgcgcaacc gcctgtcagc ttcaaattcg gccagtttcg
     3061 ctcgcatggt ctgccggacc tcaagccgcg ccgcatcggg cagcatcaga tatgtttgcg
     3121 ggatggagcc aatgcccgcc tcgatcgaat cccaatattc gtcgaagctg ccatagctgt
     3181 cctcacgcgt ttgccgttcg attagaacct gctgaaatcc agcattgatc agcaaagttt
     3241 cgagccgggc cgggtcgcac agcgagaaag acagatggac agtctgctgc tgcgcgggca
     3301 gaaggcggcc gagcagacca gcaaggatgc cccacatcgg ggcgcggtcg gggctggaga
     3361 tgacgcatat tccggcgcga cctccgtcgc gaaggacacg tcggaactcc ttcaatccga
     3421 ggccgggatc tggaaagaac tgcaaaccaa gctggcagac aacagcgtca aagcagctat
     3481 cggcgaacgg cagtgcttgc ccgtccgccg ctattggcag aaaggtcggc tcattcaatc
     3541 ggtcgcgagc acattcgagc atgggcggag cgatatcggc gccgatcagg cgtcccgatg
     3601 gaccaatggc cgacgcgata gcgacagcgg cttccccggt tccggtgcac atatcgagca
     3661 cctgatcgcc tggctgaaca tcagcggctt cgagcacaag cggtacgaaa agccgggacc
     3721 accggccggt gaattgctca tacgccgatg catcgacggc gtggcctggt ctgacgtgga
     3781 accccggtgc acgctcggtc ttcatggtct tctccggcgt cagaattcct cgcgggagtc
     3841 agatgcagga taccgaccgc cggctttgca ccactagctc acgacgcgca tcgtcggcag
     3901 gttctccggc accgtcagct tgcagccaac gctcgcgtgg acctcatcga gcgtgacccc
     3961 gggtgcgatc tcgcggatgc ggaagccgtc gccgatcggt tcgaacaggc catagttggt
     4021 ggcgaccagc ttcaccacgc cgcgcgcagt taccggcagg gcgcactgcg ccaccagccg
     4081 cggtgcaccg ctgcgcgttg cctgctccat gatgatgaac acacgcttgg cgcaggccgc
     4141 gaggtccatc gcgccgccga tgccaccacc tttctggccc gagatcttcc agttggcgaa
     4201 gtcgccgttg cacgcgacct catacgcgcc catcacggtg acatcgattc gtccgccgcg
     4261 gatgatggcg aatgagtcgg cgtgatgcac cacggccgcg cctgccttca aggtgacgtt
     4321 ctggccgccg gcgttgacca ggttcaggtc ctcctcgccg gccggtgcga tcgggccgta
     4381 gccgatgacg ccgttctcgg aatgataggt gatgtcgcgg tcgccgatgt cggtgttgga
     4441 gcacatcgtg ggaatgccca caccgagatt gacgatccag ccatcctgga attcgaaggc
     4501 gatgcgatcc gccatctgct gacggttgat acctttctgg gttccgctca tcgacggcgc
     4561 tcctgacggc gttgcggcca aaggccttcg gggtgaggcg gcactttcac cacgcgggtc
     4621 acaaagatcc cggaggtatg gacctggtct ggatcaattg cgccggcggg caggatgtcc
     4681 tcgtccactt cggcgatggt gacgcgtgcc gctgtcgcca tcaccgggca gaaattgcgc
     4741 tgtgagcggt ggaattgcag gttgccgaag gtgtcggcac gcgtggcgcg gacgaacgcg
     4801 tagtcgagcg gtagcgcgta ttccagcagg tattcacggc cgccgatgac acgggtctcg
     4861 cggccctgcg cgagctcggt gcccacggcg gtgggcgtgt agaacgcggg gatgccggcg
     4921 cccgccgcgc gcagacgttc cgcgagcgtg ccttgcggga cgagttcggc atcgacttcg
     4981 ccggactcgt agtatttggt gaagggcagc acgtcggtgg ggcgcgtcgc ggcggtgaac
     5041 gcgcagatca ccttcttcac ctggccgttc tccaccagca tgcccatatc cggcatgcgc
     5101 ggctgctgcg cgccgccggt tgtgttgccg acgagcgtca gattcttcgc cccgtgctca
     5161 agcaacgcgc ggatcaggtt gaacggcgta cctggcatgg caaatccgcc gaacgcgatg
     5221 gtggcgccat ccgggatgtc cgctaccgca tcgcggaacg tggcgacctg cttcgatttt
     5281 tgtgtcatct ccccccgctg tgcgtatggc gtgcagggat ggaggagtat ggacgagacc
     5341 gagtcaagac gtcagcaggt gctacgatag cgatccctgg ttgcctctca cccgccgcca
     5401 ttagggtcag gcggcgcaat cgaatatcgc gtcagattcc gtacgaggaa tgccacgagt
     5461 atgctgtggt cctcggcgtg agactctggt tttacggccg cgacctcaac ggcacgatgg
     5521 cttgaacctg cacgtggatg gcgtcgcatg gccacattcc gatgcaccac tgaaggagtt
     5581 gatggctcga tcgggtcgac aattgatgcc tgcgacgtca cctctcgcag cggaatgctc
     5641 actgcgggtc cggcaacatc ccacgagccc gggtcaggca atgccggctc ggtcgagaac
     5701 tgaaccggcg gcaacgacaa gcgtattgca ggcgcaacgg cgttcgacgt ggctacgccc
     5761 gggggagaga ctattgtcgt tcgcgatcct ggaatggacc cgtcatgccg ctgaaccaat
     5821 aggatcatca tcaccgcagg tacgcagaat agtccggaaa gcaatagccg ccacatcagt
     5881 catggcccct cagggattcg tatttggatg ccaacctgca ccatttcccc ctgggaagtc
     5941 actggcaaaa agccttccgt tccatgttgg cggaatggga ccatttattg cgtcgtcaca
     6001 ttcttcggac attggtgaat tgcgccactc cacccttcag gatgcccgtc agattcttcc
     6061 gataggctcc ggcgaagaag aacgcgccga gcggtatgta tggaacatcc tggaaggcct
     6121 gaagctggat ttcgcggcac aggcgcttct gttcctccag atcggttgcg tcgatccatg
     6181 cggagcgcag ttcctccacc cttggctcgg tcggccagcc gaacagagac tgttttccta
     6241 acccgcgcag gaaactgtgc gccgccgggc tcatcgctgc gaagccaggc gcgtagttcg
     6301 cgttcaggct ccagccgccc ttgtccggcg gcgcctgtga aagcagccgc tgcgccaccg
     6361 tcgcccagtc caacgcctgg tagtcgaggt tcatgtccag cttgtggaaa acttcggccg
     6421 cgacctcact catcgcgttg atcgccggaa gatctgtcgg caccaggaac acgatcttct
     6481 cgctgttgta gccggccgcc gccagatcgc gcttcacctt gccaagatcc cgcgggctgt
     6541 tcagtacgtc catgcccgcg tcgttggcga attgcgagag tgggctgaag atgccgacgc
     6601 ccgcgcgcca catggaggga tcttcgccgg cgaccgcctg catcatgtcg gtctggctga
     6661 acgcacccag cagcgctcgc cggatgccgg cgttatcgaa cggcggatac aggtggttga
     6721 agcgcaggac cgcctggtag ccgagcgtat cgatgatatc gactttcacg tccgggttct
     6781 tgcggagcag aggcagcagg tcggtcgtcg gctgctccca ccagtccatc tcgcctgatt
     6841 gcaatgcgcc ggcagcggtg gcgggatcag gaatggtgtg ccactcgacc cgatcgaaat
     6901 aggcgatctt cgggccggcc gtgccaaccg gcggaccatc ggtgcgcggc acataatcgg
     6961 tgttgcgtgc atatacagca agcgaacctg gcacacgctc cttcatcaca aacttatagg
     7021 gaccactgcc gatcagttct gtcacctgct gcgccggcgc gttcgcggcg agccgctccg
     7081 gcatgatggc cgatgcgtat ggtgtcggct tcgccagcag atcgggcaac aggcggaatg
     7141 gccgtttcag ccggaactgc agcacagtgt cagagatcgc cgacaggtca tccacctggc
     7201 tgaacacgga aatcgcgaaa acatcgcgct tgccccaacg cttcagggag gcaacggcat
     7261 cgcgtgccag cacgggcgtg ccatcatgga agcgcaggcc ttcgcgcaga gtgatcttcc
     7321 aggtcttgcc gtcgtcctcg acgacgtggc ccgcggccat ctgaggccga ggccgcgcgg
     7381 cgtcgtccac gccatacagc gtgtcgaaca ccatgtaggc gtgattgcgc gtgaccagtg
     7441 ccgaggcgaa gtgcgggtcc aacaacgcga tatcggcctg cggaatgaat ttcagcagct
     7501 tggtgtcgcc agccgcgcgc accgcggggg cagcgagtgt gagtgcagcg gaaccagcca
     7561 ggaaatcgcg gcgcttcatt gcgggttgct ccttgcagcc ttgcgatggt tgcaagtgaa
     7621 acggactgtg gcaagtgggc gcggattgcc aggctgcggc ttcggttcca ggatgcccgc
     7681 aaaggtcaat gaaccgggag ggcggtcaaa tgaacagcaa gccgcaactg cgcccattgg
     7741 gcgaagcatt gggtaccgaa gcgcttggca tcgatctgtc cgagccactc gatgatgcaa
     7801 cgttcgactg ggtggcgaac gcctttgccg agcatccggt gctggtgttc cgtgatcagg
     7861 acatcggtgc ggcgccgctc gccgaattcg gccggcggtt cggtgcgccg cgcaggcacg
     7921 cgctgatcaa gtaccgccac gccgattgcc cggaggtgtc atggctgacc aatgtcgatg
     7981 aagagggcaa ggtggactgg tatggcgtga agcgcgccac agactggcac accgactcaa
     8041 cgtatgaggc ggaattaccg ctgcttgcga tcctgcacgc gaaggaggtg ccgtcggaga
     8101 aaggcggcac gatgttcgcc gatatgcgtg ctgcctacga tgcactgccg gatgcgcgga
     8161 agcagcagct cgaggggctg accgcgctgc atggccgcag cgtgggtccg gcgggcgaga
     8221 aactctatgg cgccgacaag gggatcacgg tgaagctgcc ggagaacgtg cagtggcccg
     8281 cggtgacgcg ccatccggtg acggggcggc aggtgctgtt cgtcaacccg atgcacacgt
     8341 acgggtttgc cggcatgacg caggacgaag cctggccgct gatcgaggag ctggccgagc
     8401 acgccacgca ggatcgcttc gtctattatc accgctggcg cgtcggcgac gtgctgatgt
     8461 gggacgagcg ggcaaccatg caccgcggcg ccggcgacta ccgccccgag gagcgccgga
     8521 tcatgctgcg gacgatcgtg tatccgggct gatgcgccga ccccgggggc tgcggctccg
     8581 ccgttacctg ggcctggacc cagttccggc atgacggcgg gcaatgccgg ccgacgtcat
     8641 gccgcacgag ctatacgatc ggaatgcaga ggccgatgcc ggtcttggtg accttgccgc
     8701 caccgagcga ttcggccttg tagttctgat agtagaaatg ccaggtgttg cccgcgcgaa
     8761 tccctacaac ggtggagcgg tagtcctcaa gcccgatcgg cgcacgcttg tagtctgtct
     8821 tgaccagcat gtgggccggc agggaaccac cgaatttggt ggtcatgtcg gtgtccatcg
     8881 cgttctgatc ggcttgctgt gtgaccgggt ttgcgaagtt gctgtgcgcg acggacggac
     8941 tggggccgtg accgcgccag ccaaaggtgc agccgttcag cgtcggcgtg aagaagtact
     9001 cggcattgat gcccagcttg ccgcaatagg tccgtccgcc gccccagggc aggaaatatg
     9061 cgcggatcgc gtgcgtgctg ccggtaactt tgcgggttcc cgcgctaccg tcaggtttgt
     9121 tatcggtttc tcgcagcagg cgcagctcat attccttggc gctgccgccg gcctcggtct
     9181 gcatcgagcc gatcttgcgg agatcaaatt cctgcaccgg aagttcggtg tcgacggtgc
     9241 caaggttctg gttgaatgga ttggcgccac cggtggcgcg gctcgcattg tagacgataa
     9301 ggtgatcggc caaccacctc tctt
//
LOCUS       DATGEM010000595         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1093103, whole genome shotgun sequence.
ACCESSION   DATGEM010000595 DATGEM010000000
VERSION     DATGEM010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093103"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VKQ27_18195"
     CDS             <1..399
                     /locus_tag="VKQ27_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90919.1"
                     /translation="ELPPVYSGPTPANPFGSQAFDIFDVMEQVEEAAQPRAATAASVE
                     PEPAPAAEPEATSTEAQAAELAPATHEPKPVTEAEPPPEPDLAEPEPSAAAEPPVPAN
                     DVAAEPAVKPIIIGGEQEAVVEKKRGWWRR"
     gene            complement(498..965)
                     /locus_tag="VKQ27_18200"
     CDS             complement(498..965)
                     /locus_tag="VKQ27_18200"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HLY90920.1"
                     /translation="MARPHTLWIAIADGEHARFVQPGAGNVLHTVSAVDSASAHLRSR
                     DIGTDRPGRSFESAASAHHAVGERHDLHRQEKDRFAQFVAEELNAAAERDTFDELLLV
                     APAHVMAELRRALNDGTVKKLVGMLEKDLVKTPDHELWLHVKGWVSEMHSPII"
     gene            1063..1950
                     /locus_tag="VKQ27_18205"
     CDS             1063..1950
                     /locus_tag="VKQ27_18205"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLY90921.1"
                     /translation="MRIHLDRKGRQLATVAGLCLLLAAMFTAVSYSVTLYRLFCQVTG
                     AGGYTARAKSDTAAQSSRVVTVAFDTNVAPGMPWRFEPVQNSVRVHLGEEALVFFRAE
                     NLSDTAIVGHATFNVTPDRAGPYFKKIQCFCFTEERLGPHKSVEMPVDFFVDPRMAKD
                     VSDNDVDHIELSYTFFPSRQPEGAADLARFDNATPSAAAGGKLFATTCSACHAPDRNK
                     VGPALEGVVGRAAGSVSGYPYSAALAHAHVVWNPTTLDKWLANPRGFIPGATMPFSLP
                     DEMRRRDIIAYLETLKPNS"
     gene            complement(2096..2881)
                     /locus_tag="VKQ27_18210"
     CDS             complement(2096..2881)
                     /locus_tag="VKQ27_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HLY90922.1"
                     /translation="MSDQPRPIAEEELHAYVDGCLEADRQRAVLRYLQEHADVSRRIA
                     AYQAQREALREAFGPMAAEPIPSRLGLERLVQERLGRRWRFWPAAAACVLLGLGLGGV
                     GGWFLHGGIPPNLATIALLTQAAVANHVVYTADKRRPTELGATQRDDLARWVSNRLNH
                     EVVPPDLSGEGYSYMGGRLAATPDGPAGLFMYASARGVRLTVFVLPLHSANSAPIQHV
                     DFAHVDGCAWIDKSIGYTVVGDLPPAELRRLAEVVRRQFEGTM"
     gene            complement(2878..3399)
                     /locus_tag="VKQ27_18215"
     CDS             complement(2878..3399)
                     /locus_tag="VKQ27_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY90923.1"
                     /translation="MTVSGVLTRIEGCIPALRRYATALLRNREDADDLVHDCLVRALD
                     KLHTRRDDADVRAWLFAIMHNLFISQLRRQKARPAGDPLDETHEATLTQRPDQEANLH
                     FRDLVGALNRLPVEQRSVVLLVSVEDLSYAEAASVLGIPIGTVMSRLARGRERLRQMI
                     EGEEVRPVLRRVK"
     gene            3556..4713
                     /gene="dgoD"
                     /locus_tag="VKQ27_18220"
     CDS             3556..4713
                     /gene="dgoD"
                     /locus_tag="VKQ27_18220"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HLY90924.1"
                     /translation="MKITKVEPLLLDRFLYVRISTDAGIIGIGESGTWGQLEASAASI
                     AKYGEYLIGKDPFPIEHHWNVMLRANHFTGGAITGAVSAIDIALWDIKGKYFNVPVYE
                     LLGGRMRHKARLYGHVKGRTIEKLVEEAVRLKEAGFTALGHLNPLLDESEDEPYYKPH
                     AAKIEEAIDNVRRLREAVGPKVDLCIEIHRRLTPPEAIALARGIEKYTPMFYEDPLRP
                     NSFDAMADVARHIDIPIATGERFTSIYQFQTLLTRNAVQYLRPDACLVGGITGMKKVA
                     AIAEAHDAWVVPHNPLSPISTAACLQIAACIPNFAIQEYPSRTPDLDGGKELLGNDLA
                     RGLSEQIDGFIAIPEGPGIGVELVDGVEKQFPYRKRPIKMRPHADGSVVDM"
     gene            complement(4738..5310)
                     /locus_tag="VKQ27_18225"
     CDS             complement(4738..5310)
                     /locus_tag="VKQ27_18225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY90925.1"
                     /translation="MISVRRARPADAISIGSVHVAAWRSAYPGILPDDFLARLSVPRQ
                     AAHYDAAIRSSSTGVFVASASGNDVPPGSGSRIMGFATAGRARTKLAGGGVLAEGEVE
                     TLYVLDDWRDRGVGRKLMRAAAAWLAEAGCKSCFVWVLRDNPSRWFYQRLGGKPTAEA
                     VIQFAGQKVPQTAFVWDPIERLLAASPQES"
BASE COUNT         1041 a         1935 c         1607 g          870 t
ORIGIN      
        1 gaattgccgc cggtctattc cggcccgacg ccagccaatc cgttcggcag ccaggccttc
       61 gatattttcg acgtcatgga gcaggtcgag gaagcagcac agccgcgggc tgccaccgcc
      121 gcttccgttg aaccggaacc agcgccagcc gcggagccgg aggcaacctc taccgaggct
      181 caagcggctg aactggcccc tgccacacac gaaccaaagc ctgtgaccga ggcggagcca
      241 ccgcccgagc cagatctggc ggaacccgag ccgtccgccg ccgcagaacc gccggtgcca
      301 gccaacgacg ttgccgccga accggcagtc aaaccgatca tcatcggagg cgaacaggag
      361 gcggtcgtgg agaagaagcg cggctggtgg cggcgataac acgaaccgcc gcggcaatct
      421 ccgttcagcc agcatgcacc ggcgcaggcc ggtgcatgct ttgatcaatc aagcccccga
      481 caccgcgtcg atgcgcctca aatgatcgga ctgtgcatct cgctgaccca ccccttcaca
      541 tgcagccaaa gctcgtgatc cggggtcttc accaggtcct tttccagcat tccgacgagc
      601 ttcttcaccg tcccatcgtt cagcgctcgg cgaagctcgg ccatgacatg cgccggtgcg
      661 acaagcagca gttcgtcgaa cgtatcgcgc tccgcggccg cattcagttc ctcggccacg
      721 aactgggcga accggtcctt ctcctgccgg tgcagatcgt gccgttcgcc gacagcatga
      781 tgcgccgaag cggcgctctc gaacgagcgc cccggacggt cggtgccaat atcccgtgac
      841 cgcagatgcg cggaagcaga atccacagcg ctgaccgtgt gcagaacatt gccggcacca
      901 ggctggacga accgggcgtg ctcgccatct gcgatcgcga tccaaagcgt atgaggtctc
      961 gccatccctg catcctcctt acgttgaata catgtgacac ccgaaccgat cgggctgcct
     1021 tgatctggat catccggtgg gcggggcgta tagccgcctg ccatgcggat ccacctggac
     1081 aggaaaggcc gccagctggc gaccgttgcc gggctttgcc tgttgctcgc ggcaatgttc
     1141 acagcggtat cgtactcggt gacgctctac cggctgttct gccaggtcac cggcgcaggc
     1201 ggctacacgg ctcgcgccaa gagcgacacc gcggcgcaat caagccgcgt ggtgaccgtc
     1261 gcattcgaca cgaacgtggc gcccggcatg ccatggcgct tcgaacctgt gcaaaacagc
     1321 gtgcgggtgc atctcggcga ggaagcactg gtgttcttcc gcgccgaaaa cctgtccgac
     1381 actgccatcg tcggccacgc cactttcaac gtcacaccgg accgcgccgg tccttatttc
     1441 aagaagatcc agtgcttctg cttcaccgag gagcggctcg gtccgcacaa gagcgtcgag
     1501 atgccggtag acttctttgt cgatccccgt atggccaagg acgtcagcga caacgacgtg
     1561 gaccacatcg agctgtccta caccttcttc ccgtcacgcc agccggaggg tgcggccgat
     1621 ctcgcacggt tcgacaacgc cacgccgagc gccgccgcgg gcgggaagct gttcgccacg
     1681 acatgcagcg cctgccacgc gccggatcgc aacaaggtcg ggccggcact cgagggcgtg
     1741 gtcggtcgcg cggctggcag cgtcagcggc tatccctact cggcggcact ggcccacgcg
     1801 cacgtggtgt ggaatccgac gacactggac aaatggctgg ccaacccgcg cggcttcatc
     1861 cccggcgcca cgatgccctt ctcgctcccc gatgaaatgc gccgccgcga catcatcgcc
     1921 tacctggaaa cgctgaagcc gaacagctag agcgtgatga ccagaggcgg aatcgccggt
     1981 ggtctgaatc acgcgataaa gcaaacagcc agagcgggat cagcgcgtcc ttcagcgctc
     2041 attccgctct aggcgcagcg ccaatccaat gtccgccgcg atgcgtccgc tccgctcaca
     2101 tcgtcccttc gaactgccgc cgcacaacct cggcgagacg gcgcaactcg gcaggtggga
     2161 gatcgccgac aaccgtgtag ccgatgctct tgtcgatcca ggcgcagcca tcgacatgcg
     2221 cgaagtccac atgctggatt ggcgcactat tggcagagtg caacggcagc acgaacaccg
     2281 tcagccgtac acctcgcgcg ctggcgtaca tgaacagtcc agctggtccg tccggcgtgg
     2341 cggccaacct accgcccata tagctgtagc cctcgcccga aaggtcaggc ggcaccacct
     2401 cgtggttaag ccggttcgag acccaccgcg ccaggtcatc gcgctgagtg gcgccaagct
     2461 cggtcggccg gcgcttgtcc gcggtataga ccacgtgatt ggccaccgca gcctgggtca
     2521 gcagagcaat ggtggccagg ttgggcggta tcccaccgtg caggaaccag ccgccaaccc
     2581 cgcccagtcc gagtccaagc agcacgcacg ctgccgcggc gggccagaac cgccatcgcc
     2641 ggccgaggcg ctcttggaca agccgctcca ggcccagccg cgacgggatc ggttccgctg
     2701 ccatcgggcc gaaagcttct cgcaaagcct ccctctgcgc ctgatacgcc gcgatacgac
     2761 gcgatacgtc ggcgtgctcc tgcagatagc gcaacaccgc tcgctggcgg tccgcttcca
     2821 ggcatccatc cacataggcg tgcaattctt cctcagcgat cggccgcggc tggtcgctca
     2881 cttgaccctc cgcagcaccg gccggacttc ttcgccctcg atcatctgcc gcagccgctc
     2941 acggccacgt gccagtcgcg acatcaccgt accaatggga atacccagca cgctggcggc
     3001 ttccgcgtag gacaggtcct ccaccgacac cagcagcacc acgctgcgct gctcaaccgg
     3061 cagccggttc agcgccccca cgaggtcgcg gaaatgcagg ttggcctcct ggtccggacg
     3121 ctgggtaagc gtcgcctcat gcgtctcgtc cagcggatca ccagccggtc gcgccttctg
     3181 acggcgtaac tggctgatga acaggttgtg catgatggcg aacagccagg ccctcacatc
     3241 ggcgtcgtca cggcgggtgt ggagcttgtc cagagcacgc accaggcagt catgcaccag
     3301 gtcatcggcg tcctctcgat tgcgcaacag agccgtcgca tatcgccgga gcgccggaat
     3361 gcagccctcg atacgggtca acacgccgga cacggtcacg gtcctctgct cagccacggg
     3421 atcgacggca atcgtgtctg ctccggcctg taccccccgc cataagacgg cgccggtccc
     3481 ggtttattcc cgggcggtga ctggacgtag ggacgctcac tgacggaagc tggcgcggca
     3541 taaggaggga catccatgaa aatcaccaag gttgagccac tgctgctcga tcgcttcttg
     3601 tatgttcgga tcagcactga cgccggcatc atcggcatcg gcgagtccgg cacctggggc
     3661 cagctcgaag cctccgccgc atccatcgcc aagtatggcg agtatctcat cggcaaagac
     3721 cctttcccga tcgaacacca ctggaatgtc atgctgcgcg ccaatcactt caccggtggc
     3781 gcgatcaccg gcgccgtgtc agcaatcgac atcgcgctgt gggacatcaa gggcaagtat
     3841 ttcaacgttc ccgtctacga actgctcggc ggccgcatgc gccacaaggc gcggctgtat
     3901 ggccatgtga agggacgcac gatcgaaaag ctggtggaag aggccgtgcg cctgaaggaa
     3961 gccggcttca ccgcgctcgg ccacctgaac ccgctgctcg atgaaagcga agacgaaccc
     4021 tactacaagc cgcatgccgc gaagatcgag gaggcgatcg acaacgttcg tcgcctgcgt
     4081 gaagcggtcg gcccgaaggt cgatctgtgc atcgaaatcc accgccgcct cacgccgcct
     4141 gaagcgatcg cactcgcccg cggcatcgag aaatacacac cgatgttcta cgaggacccg
     4201 ctgcgaccca acagcttcga cgcgatggcc gacgtggcgc gccatatcga cattccgatc
     4261 gccaccggcg agcggttcac gtcgatctat caattccaga cgttactaac acgcaacgcc
     4321 gtgcagtacc tgcgccccga cgcctgcctg gtcggcggca tcaccggcat gaagaaggtc
     4381 gccgccatcg ccgaggcgca cgacgcgtgg gtcgtgccgc acaatccgct gtcaccgatc
     4441 tccaccgcgg cctgcctgca gatcgccgcc tgcattccga actttgcgat ccaggaatat
     4501 ccatcacgca cgcccgatct cgacggcggc aaggaactgc ttggcaacga ccttgcgcgc
     4561 ggcctgtccg aacagatcga tggcttcatc gccataccgg aaggtcctgg catcggcgtg
     4621 gaactggtgg acggcgtcga gaagcaattc ccctaccgca agcgtccgat caagatgcgc
     4681 ccgcacgcag acgggtcggt ggtggatatg tagcggatag ccggcggcgt tccgctgtta
     4741 agattcctgc ggcgacgccg ccagcaaacg ctcgatcgga tcccacacga acgccgtctg
     4801 cggcaccttc tgaccggcga actggatcac agcctcggcc gtcggcttgc cgccaagccg
     4861 ctggtagaac caccggctcg gattgtcgcg caacacccag acgaagcacg acttgcaccc
     4921 tgcctcggcc agccaggccg ccgccgcccg catcagcttc cggcccacgc cacgatcgcg
     4981 ccagtcatcc agcacgtaca gcgtctcgac ctcgccttcc gccagcacgc ctcccccggc
     5041 caatttcgtg cgtgcccgcc cggcggtcgc aaagcccatg atgcgcgagc cactgcccgg
     5101 cggcacgtcg ttgccggatg ccgatgccac gaacacaccg gtcgaagacg accggatcgc
     5161 cgcatcgtaa tgcgccgcct gacgcggcac cgacaaccgc gccagaaaat cgtcaggcag
     5221 gatgcccgga tacgcgctgc gccacgccgc aacatgcacg gagccgatgg aaatggcatc
     5281 cgccggccgt gcacgccgca cgctgatcat cggtacttcc gggcgacaac ccggtcctcg
     5341 ctcgtcatgg ccgggctcgc ttccagccaa ccgcaacgtt cgtcatcctc gcgctcggcg
     5401 cgagaaccca cgccttcact cgccgcccta gccatcactc ccgccgctcc agc
//
LOCUS       DATGEM010000596        22009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1506131, whole genome shotgun sequence.
ACCESSION   DATGEM010000596 DATGEM010000000
VERSION     DATGEM010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 22009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22009
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506131"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VKQ27_18230"
     CDS             complement(<1..943)
                     /locus_tag="VKQ27_18230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HLY90926.1"
                     /translation="MLFNSYIFVLAFLPVALLGFVAASRLGRRVAGCWLIAASLFFYG
                     WWDVGFVPLLTGSIAGNYAAAMLLYRLARRKRAQTVVLVLAIGLNLAALVYYKYLGTM
                     LGVLDVAFTSPVLPLGISFFTFTQIGYLLDCRNDTARDRSPLNYALFVTFFPHLIAGP
                     IVHNREIMPQFADTATYGLSARNFTVGSSIFLMGLLKKCLLADPTSAVVGPGFAHPEA
                     LTLFPAWHVALSYSLQLYFDFSGYSDMAIGLARMFNVTFPLNFNSPYKARSVIEYWQR
                     WHMTLTRYLNLCLYNPIALAIRRRRPARGFATTPHGFA"
     gene            complement(956..1216)
                     /locus_tag="VKQ27_18235"
     CDS             complement(956..1216)
                     /locus_tag="VKQ27_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLY90927.1"
                     /translation="MRHTRDTILAEVADVARQVFEDPTLELTMDTTSDDVPRWDSMNH
                     ITLVVECESRFDVRFETMAIEELRSVGELVRLIERKLALAAA"
     gene            complement(1284..1862)
                     /locus_tag="VKQ27_18240"
     CDS             complement(1284..1862)
                     /locus_tag="VKQ27_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HLY90928.1"
                     /translation="MEQSPNPNTDFLTGQLLIAMPAMEDPRFSHSVIYLCAHTPEGAM
                     GLVLNRPLQKPKFDELLRQLEVAPVPPARRIQLCQGGPMDNARGFVLHTTDWTGEGSL
                     KVDDTVALTASLDVLKAIAEGNGPREGILALGYAGWGPGQLDAEIQQNAWLSVPADET
                     IVFDDAHDTKWRRALGKLNIDPLLLSGAAGHA"
     gene            2025..2507
                     /locus_tag="VKQ27_18245"
     CDS             2025..2507
                     /locus_tag="VKQ27_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLY90929.1"
                     /translation="MTIKVGEQIPSMKLMMGTPDGPKETSTDEIFKGKKVVLFAVPGA
                     FTPTCSAKHLPGFVQNIEALKAKGVDTVACIAVNDAFVMAAWGKDQGTDGKVMMLADG
                     SAAFTKAMGLELDLMGRGMGTRSQRYALVAQDGKVTHLAVEAPGGFEVSKAESILSAL
                     "
     gene            complement(2583..4622)
                     /locus_tag="VKQ27_18250"
     CDS             complement(2583..4622)
                     /locus_tag="VKQ27_18250"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLY90930.1"
                     /translation="MQEFGSIYRHGFARVAACTTRCTLADPMANAAEILDVARACDQR
                     GVALAVFPELCVSGYAISDLLQQFTLLDAVEAAIGTLVEASTNLLPLLLVGAPLRHAG
                     ALYNSAIAIHRGRLLGVVPKMHLPNYREFYEPRHFVVGDPATNGEITIAGATAPFGTD
                     LLFQAEDIRGLIVHAEICEDVWIPVPPSSTAALAGANVLANLSASNITIAKADTRRLL
                     CASQSARCVAAYLYAAAGAGESTTDLAWDGQASIFENGTTLAETERFPSSSQVAIADI
                     DLDLLCHERMQMGSFDANRHRHATPFRRITFRVDPPGGDIGLERRVERYPFVPSDPGR
                     LEQDCYEAYNIQVSGLTQRLRAIGVKHVVIGVSGGLDSTHALIVCAQALDRLSLPRAN
                     ILAYTMPGFATSAHTKANAVRLMQALGVTWQELDIRPAAERMLADIGHPFARGEPAYD
                     LTFENVQAGLRTDYLFRLANHHNGIVIGTGDLSELALGWCTYGVGDQMAHYNVNAGVP
                     KTLIQHLIRWVISSKQFAPEVSATLDAILATEITPELVPTKEGETPQSTEAFIGPYAL
                     QDFNLYYTLRHGCVPSKIAFLALNAWGDAAKGDWPPGFPPERRGAYDLATIRRWLEVF
                     VRRFFAFSQFKRSAMPNGPKVSAGGSLSPRGDWRAPSDGNANAWLAELERNVPEQ"
     gene            4763..4936
                     /locus_tag="VKQ27_18255"
     CDS             4763..4936
                     /locus_tag="VKQ27_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HLY90931.1"
                     /translation="MLRLALFFLVVSLVAALFGFGGISAASADIARILFFIAIVVFLV
                     LLVAGLMAGSTLF"
     gene            complement(5065..5442)
                     /locus_tag="VKQ27_18260"
     CDS             complement(5065..5442)
                     /locus_tag="VKQ27_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HLY90932.1"
                     /translation="MTSENPSRLIDARIKELGDWRGKTLAHVRSLIKQAVPDVVEEWK
                     WRGVPVWYRDGMICTGETYKSAVKLTFAKGASLEDPSGLFNASLDGNARRAIDIHEGD
                     ELDAGAFQGLIRAAAALNRKPAR"
     gene            complement(5455..6081)
                     /locus_tag="VKQ27_18265"
     CDS             complement(5455..6081)
                     /locus_tag="VKQ27_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLY90933.1"
                     /translation="MRRILWGRATSSNVMKVIWTLEELQLPYERLDVGGSFGKTDTAE
                     YRAMNPTGLVPTLQEDDFTLWESNAICRYLCHAHSPHSKLWPQDPHARANIDHWMDAQ
                     QTVLNRPMGVVFWGLVRTPPDQRDMKAIMQGVQDTAKAWGMVAAELAKHPYLAGDDLT
                     LADIPWGPHLHRWFSMEFDRPEVPHLRAWYDRLLERPAYKTHLAGKVV"
     gene            6175..6657
                     /locus_tag="VKQ27_18270"
     CDS             6175..6657
                     /locus_tag="VKQ27_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HLY90934.1"
                     /translation="MRSLALLSAIAAGFALGLAEASEHWGGLVPCALCLVERWPYRVA
                     IALSLIAFAVPRQLARWLLVLTVLTILVGAAFAFVHVGVELHFWPSPLPECAAPSLGS
                     GSIAERLAQMPARPAKPCDAPTFLIPGIPLSMAAMNLIYAMVFVAALTIGLWRKWRTA
                     "
     gene            6654..7187
                     /locus_tag="VKQ27_18275"
     CDS             6654..7187
                     /locus_tag="VKQ27_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HLY90935.1"
                     /translation="MNLPGDRSHESQVARIVRVDHAGEYGAARIYAGQLAVLRRRESA
                     PVLREMQAQEQEHLDRFAELIHRRRVRPTAMLPLWHLAGFALGAATAALGERAAMACT
                     VAVEEAIDAHYASQIDALDDSEAELRGTLTKFRGEELQHRDTALAHGAEQAPGYRLLS
                     AAIKAGCRVAIRISERV"
     gene            7235..8092
                     /locus_tag="VKQ27_18280"
     CDS             7235..8092
                     /locus_tag="VKQ27_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLY90936.1"
                     /translation="MLRAIVLLALLLTPGFATAEELKFATWNLEWLTVRSRESADLPR
                     DAHPKRAEDIETLNHYAADLNADVIAIEEVDGAAVAARVFPADKYSIHMSQDQDVQRV
                     GVVVRRGIRYSVNPDLTDLNINRHLRSGVDVTLRLDPAPLRILAVHLKTGCLDRPLSN
                     RGSGDCTELRQQATVLAGWIKARQSEGAAFIVLGDFNRHMDERDKFWPALARAAPLIR
                     ATAGHSSPCWGGEAFIDHIILGGAARDWLVPDSLRVLTYRETGPEWKDRLSDHCPVSV
                     RLRVPDPAR"
     gene            8122..9210
                     /locus_tag="VKQ27_18285"
     CDS             8122..9210
                     /locus_tag="VKQ27_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLY90937.1"
                     /translation="MKITGITTKEYRWPRNKPIANGKHTYTHVTFAVVEIETSDGITG
                     IGLGGGGEIERATVARLAPMLIGQDPVNVERLWHQMWEPKLIGRRGLTTRAIGAIDIG
                     LWDIRAKLAGLPLYRLLGGFRDRVPTYIAGGYYEEGKGLRELAREMEDNVRMGAKAVK
                     MKVGAVSIGEDVARIRVAREAVGPDVKLMVDANCAYRYYEAIQLAKRIEEYDIFWFEE
                     PVMPDDYDGHRKLAQATSIPIATGENEYTRYGFRDLIKQECAAILNADAKVLGGVTEF
                     MKVAALAQANDLDIAPHGSQDIHVHLVAAISNGLILEFYRDTVDPMWGRVYRHTLRLN
                     DDGTVSPPDVPGIGADPNYEGLAEFRVA"
     gene            9274..9705
                     /locus_tag="VKQ27_18290"
     CDS             9274..9705
                     /locus_tag="VKQ27_18290"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLY90938.1"
                     /translation="MARILVIHGAGMNMRGKVQTEIFGPMTLPEYDERIRSYAAELGV
                     EIEIFHSNIEGDVINKLYEANDKGFDAALFNPAGFSRGYPALVAAINQVKFPTIELHI
                     SNPVRRGPVSDTATVSQGMVTGFGIAGYYLALRGLRDTLKK"
     gene            9861..10415
                     /locus_tag="VKQ27_18295"
     CDS             9861..10415
                     /locus_tag="VKQ27_18295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY90939.1"
                     /translation="MTSKRDLIKSEATRLFVAHGVDAVSVRDIAAACEMKASNLYAHF
                     ASKDAMVAELFREGYAEYGAVLAEAAAGAEPFQVRLDRMIRAICRLHDEDVVRFRFLI
                     LTQHAFLREVPHDDHNPVEIICRAVAAAMEAGEIPSREPELMAMAVIGIIVQPATACL
                     YGRLDGGLVERAGEIVAITWRALS"
     gene            10440..13865
                     /locus_tag="VKQ27_18300"
     CDS             10440..13865
                     /locus_tag="VKQ27_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HLY90940.1"
                     /translation="MSASPLGLLNTRRMWPLALTQSCGALNDNLVKNAMVVLALFQLG
                     IGGTGLSALAGALFIAPYILFSATAGKLADRFSKPRLIVVYKLAEVALMCGAAVTFLT
                     ESVTGLLLLLAGLGLQATLFGPVKYGLLPEHLVEHELVAGNGIIEATTFLSIVIGTVA
                     GGALVQLPQGTVWVGATGIALALVGLFGAWRIPPSPAADPSLRISANVLTETWRVTRH
                     SASIRAIWLSILGLSWFWTMGATLMTEFPVVARDTLHADGTVLTLLLTVFAIGVGVGS
                     IQCARLLHGDVSPRLVPFAALGISLFCWDFASASGSAGSIANAWVALSGFAGWRMIAD
                     LFLLAACGGVFSVPLYAIIQDASAPAERSRMIAANNIMNALFMVAGAGATAGLAACGL
                     NSPQVLHVAAVANLLVAIWIVRILPQEFYRAILRWYFDRFHHVRVTGLETYKAAGDRV
                     VIVANHQSYLDALLIAAYVPDSPTFAIHTSQAAKWYFKPFLRVVETFPVDVQSPYAVK
                     RMVEAVRDQGRKLMIFPEGRMTRTGALMKVYEGAALVADKSHAKIVPISIEGLQFSRL
                     NRMGDKLHRRWFPRLRLNILPPVTLAPPGAEQLTHRQRREVIGRALQDVMVNAVFRSK
                     DTGKSLFAALLDARDAHGAHTPIIEDIERIPISYDRLVVGSAALGRALTGLTAESHVA
                     LMMPNAAATVVAFMGLQAYGRVPCMLNISAGAASMLSACRAAGVRSVISSRTFVEKGR
                     LSKVVERMEAEVRFIWLEDVRAAIGLRAKLRAKWDARRARRLPGASTDPDSPAVVLFT
                     SGSEGAPKGVTLSHRNLIANCAQLASVIDFHGGDIVFNAMPMFHAFGLTGGTILPLVS
                     GVRTFHYPTPLHYRVVPALIYDTDATICFGTDTFLNGWARYAHPYDFYAMRYIFAGAE
                     KLREETKHLFADRFGARILEGYGATETAPVLAINTAMHSRNNTVGRFLPGIEWRLEPV
                     AGIEQGGRLYVHGPNVMLGYLRDTAPGVLEPPPGGWYDTGDIVAIDAAGFITILGRAK
                     RFAKIGGEMVSMTAAEMLIAALWPNAQHAVINLPDARKGEYLLLVTTRPNAEPRDILT
                     SARERGIPEIMVPRSIMQVPAVPLLGTGKVDYPAVQRMVEAAPAPQPQETVPA"
     gene            13998..15059
                     /locus_tag="VKQ27_18305"
     CDS             13998..15059
                     /locus_tag="VKQ27_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HLY90941.1"
                     /translation="MPELNRLPLRSVLGSLVLIAVVIGGGAAAFAYTAGWFSPQRLSG
                     ARLVAAFTPPGDKPPLGHRRNHAKGICFTGTFEANGEGEALSYAQVFSHGAYPALGRF
                     NLGTPHPDAPDSTVRVRGMGLSITTPDGQVWRAAMINAPVFPVSTPQQFYALLMASGS
                     KDPKAMPDFIAANPEFAPFGAWATKGTWTGSYAEERYNSLDSFVFTNSIGVDHAVRWS
                     LLPEAQVAPLPPDELAKRGSDYLEQEIVQRVQAAPQRWTMTVTVAASVDPTSDPSKAW
                     PDDRRTITVGTLVVQRIEAEADGPCRDVNFDPTILPAGMRTSNDPFPAARSAAYAKSY
                     DLRTAESADYPHTPTEAKP"
     gene            15056..15631
                     /locus_tag="VKQ27_18310"
     CDS             15056..15631
                     /locus_tag="VKQ27_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLY90942.1"
                     /translation="MNHVEPRFTAVQRLLHWVMAVCILAMLLIGVGMESTVMPKYLTL
                     VAIHKPLGIAILVLALLRLAERLRIGTPPLPLDLPEPMKLAAHLSHYALYILMIGMPL
                     LGWAMLSAAEYPVVLYGSVHLPSIVPQSDSLHALLWDAHYYLAFALFALILMHIAAAL
                     FHLLVRRDGVFQQMAPMHLHEEDPEEIVVAE"
     regulatory      15724..15960
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15999..18290
                     /gene="metE"
                     /locus_tag="VKQ27_18315"
     CDS             15999..18290
                     /gene="metE"
                     /locus_tag="VKQ27_18315"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HLY90943.1"
                     /translation="MTIASNLGFPRIGARRELKTALERFWSGMIDEAALNATAQELRA
                     RHWRTQLGSRISHVPSGDFSLYDHVLDTACMVGAIPPGYGWSEGPVSLNTYFSLARGA
                     RGETGLPALEMTKWFDTNYHYLVPRLHPAQRFTLTGNRPLAQFIEAAALPMRTRPVLL
                     GPVSFLLLAKTIDGSGALELLPALLPVYTAILNELADAGAIWVQIDEPALALDLGAKA
                     KAAFNTAYAALAAAPAPNMLVASYFGALADNLPVVAALPVAGLHLDLVRGGHRLDEAL
                     TAIPVDRWLSLGLVDGRNVWRTDLRSALATLQQIATARGAQRLMVAPSCSLLHVPVDL
                     AQESKLDPEVKSWLAFATQKLAEVAALARGLDEGEAAIARELEASDAAQRSRHGSSRV
                     HRNEVAERLAATTPAMERRASAYPQRRTGQQARLKLPPFPTTTIGSFPQTSDVRQARA
                     DRGRGNATEAEYAASIEHWIDDAVQWQERIGLDVLVHGEFERNDMVKYFGEQLDGYAF
                     TQHGWVQSYGSRCVAPPIIWGDVARPGPMTVRWSSYAQSRTRRPMKGMLTGPVTMLQW
                     SFVRDDIPRETVCRQIALAIRDEVMDLEAAGIPIIQVDEPAFREGLPLRHAEWAAYLG
                     WAVACFRLATAGVADATSIHTHMCYSEFNDIMPAIAAMDADAISIETTRSRMELLNAF
                     GAQGAYPAEIGPGIWDIHSPRVPDAAEMAELLGLARQRLADWQIWVNPDCGLKTRKWE
                     EVRPALENLVAAARQMRDAMAGG"
     gene            complement(18302..19033)
                     /locus_tag="VKQ27_18320"
     CDS             complement(18302..19033)
                     /locus_tag="VKQ27_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90944.1"
                     /translation="MRLDGQAAIVTGGASGLGAATAERLRRAGCRVAILDINPGADGI
                     ACDVADAASAEAAMAKAREMHGPARILVNCAGVGTAGRIVGREGPMPLAAFERVIRIN
                     LIGSFNLLRLAAADMTTLDPMDDGERGVIVNTASIAAYEGQVGQPAYAASKAGVVGMT
                     LPAARELARFGIRVVTIAPGLFHTPMVGALPEETQRALGEAVPFPQRLGEPGEYAALV
                     EHIATNRFLNGETIRLDGALRMQPR"
     gene            19114..20124
                     /locus_tag="VKQ27_18325"
     CDS             19114..20124
                     /locus_tag="VKQ27_18325"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLY90945.1"
                     /translation="MHFRITALLCALLLSIAGAQAQDKTVQLKLSSWVPPAHPLNPSL
                     QAWAADIEKASGGTIKATLFPSEQLGKAMDHYDMARDGISDFSYVNPGYQPGRFPIIA
                     AGELPFLVANATGGSAALDAWYRKYAPTEMKDVHFCFAFMLDPGTFHSRSRKIVVPED
                     IRGEKVRPADATMGAFVTLLGGTNVQASAPEAREVLERGVADAITFPWHSTILFGLDK
                     VVKYSMDMPFFSTTFVWVMNKAKYDSMSPAQKSVIDNHCTTEWAEKVVTPWANWERAG
                     RELLIAEPGHDVYKLTPEQQAAWRKAAEPLKAKWAAQVKDADKVYGELVDELKKRGAL
                     AE"
     gene            20155..20715
                     /locus_tag="VKQ27_18330"
     CDS             20155..20715
                     /locus_tag="VKQ27_18330"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HLY90946.1"
                     /translation="MSDLEREFARKAAAEGAARSRMDRFIDAIELLAAIFVGIVAADV
                     FISVLLRYFFAVSIPDSYDFGKMLLGILIFWGIAATSYRGTHITVDLVWAAAGPRGKR
                     LIDIFATLVLLFVVSVQTAMLFDKVRQTYEENVLTFDLHLPTWPFALIAWLGDVSAVL
                     LIAIRTYRLIFHPEDVADAGVVRTVE"
     gene            20712..>22009
                     /locus_tag="VKQ27_18335"
     CDS             20712..>22009
                     /locus_tag="VKQ27_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HLY90947.1"
                     /translation="MIELGSDAVAIIGFIVLFALMLLRVPVGMAMGLVGVTGFGYLVG
                     GMPALKMVGQTSMRTVTDYTFGVIPMFLLMGAFVSRSGMSRELFQAANKFVGHLRGGL
                     GIATIAACAGFAAISGSSVATAATFSTVAYPEMRQYRYPQSFSTGVIAAGGTLGAMFP
                     PSIVLAVYGLITQQDIGKLFMAGVLPGLLAVSMYMATIGVIGAVRPAYLPAAPRSSWR
                     ERLQGLREVWAPLLLFAFVIGGLYGGLFTPTEAGGVGAGGAFLIGIARGRLSRTDILE
                     SLLQATRTAAAVFTILIGAILFGYFLTVTQTPQKVTEFVTGLGFGRYGVLVLIMLMYL
                     ALGCLMDAMAMIILTVLIIFPVITTLGFDPVWFGVIIVMTVELGLIHPPVGMNVFVIK
                     TVVKDVAFSTIFIGVIPFVITDIIRLAILIAFPALAIWLP"
BASE COUNT         3852 a         6996 c         7205 g         3956 t
ORIGIN      
        1 cggcaaagcc atgcggggtc gtcgcaaacc ctcgcgccgg gcgccgccgg cggatcgcga
       61 gcgcaatcgg attgtataga cagaggttga ggtaccgtgt cagcgtcatg tgccagcgct
      121 gccagtattc aatcaccgat cgcgccttgt agggcgaatt gaagttgagc gggaacgtca
      181 cgttgaacat tcgcgcaagc ccgatcgcca tatcggaata accggagaaa tcgaagtaga
      241 gctgcagcga gtaggacagc gcgacatgcc atgccggaaa tagtgtcagc gcctcgggat
      301 gcgcgaaccc cggtccaacg accgccgacg tcggatctgc cagcaggcac ttcttcagca
      361 gccccatcag gaagatgctg ctgcccacgg tgaagttccg cgccgacagc ccgtaggtcg
      421 cggtgtcggc gaactgcggc atgatctccc ggttgtgcac aatcggtccc gcgatcagat
      481 gcggaaagaa cgtgacgaac agagcgtaat tcagcggcga ccggtcacgc gccgtgtcgt
      541 tgcggcagtc gagcaggtag ccgatctggg taaaggtgaa gaagctgatc ccaagcggca
      601 gcaccgggct ggtgaacgcc acatccagca cacccagcat cgtgccgaga tatttgtaat
      661 agaccagcgc tgccaggttg agcccgatcg ccagcacaag caccaccgtc tgcgcccgct
      721 tccgccgtgc gagccgatac agcaacatcg ccgccgcgta gttgccggca atcgagccgg
      781 tcagcagggg cacgaacccg acatcccacc agccatagaa gaacagcgag gcggcgatca
      841 gccagcagcc cgcgacacgc cgccccagcc gcgacgcggc gacgaagccg agcagcgcca
      901 ccggcaggaa agccagcacg aagatgtacg aattgaacag cacggcggac gcgacctatg
      961 cagcggccag cgccagtttc cgctcgatca gccgcaccag ttcgccgacg ctgcgcagct
     1021 cctcgattgc catcgtctcg aaccggacgt cgaagcggct ttcgcactcg accaccagcg
     1081 tgatgtggtt catcgagtcc cagcgcggaa cgtcatcgct ggtggtatcc atcgtgagct
     1141 cgagggtcgg atcctcgaat acctgccgcg cgacgtcagc cacctcggcc aggatagtgt
     1201 ctcgggtgtg ccgcataatc cgcaaccttg ggttgtcgga cacccattac gcgccagatt
     1261 ttttgccgcc gcgttaacgc acgctacgcg tggccggcag cgcccgacag cagcagcgga
     1321 tcgatgttga gcttgcccag cgcgcgccgc cacttcgtgt cgtgagcatc gtcgaacacg
     1381 atggtctcgt cggccggaac cgacagccac gcgttctgct ggatctcggc atcgagctgc
     1441 cccggccccc agccggcgta acccaacgcc aggatgccct cgcgtggacc gttcccctcg
     1501 gcgattgcct tgagcacatc gaggctggcg gtcaacgcca cggtgtcgtc gaccttgagg
     1561 ctgccctcgc cggtccagtc cgtggtgtgc aggacaaagc cgcgcgcatt gtccatcggc
     1621 ccaccctggc acaattgtat ccgccgggcc ggcggcaccg gcgcgacctc aagctgccgc
     1681 aacagttcgt cgaacttcgg cttttgcagt ggccggttca acaccagtcc catcgcgcct
     1741 tccggcgtat gcgcgcacaa atatatgacg ctgtgcgaaa aacgcggatc ttccatcgcc
     1801 ggcatcgcga tcagcagctg cccggtcagg aaatcagtat tcgggttggg actctgctcc
     1861 atgcttacga atctggtccc gcttgcccgt gaacgcaagc gtccagagtc gggggcaatt
     1921 cgcgatcagt gcccggttct gtacgttcca cccccacccg tgactccggc cgccgggaag
     1981 gctaggagtc gcgggctggc aagccagagg ggagctagga tcaaatgacg atcaaggtcg
     2041 gcgaacagat tccgtccatg aagttgatga tgggcacacc ggacgggccc aaggagactt
     2101 ccacagacga gatattcaaa ggcaagaaag tggtgttgtt tgctgtgccg ggggcattca
     2161 cgccgacgtg cagcgccaaa cacctgccgg gttttgtgca gaacatcgaa gccctgaagg
     2221 ccaagggcgt ggacacagtc gcgtgcattg cggtgaacga cgccttcgtc atggccgcct
     2281 ggggcaaaga ccagggcacg gacggcaagg tgatgatgct cgccgacggc tcagccgcct
     2341 tcaccaaggc aatgggcctg gaactggacc tgatgggccg cggcatgggc acacgcagcc
     2401 agcggtatgc tctggtcgcg caggacggca aggtgacgca cctcgccgtg gaagcacccg
     2461 gcggcttcga ggtcagcaag gcggagtcga tcctctccgc tctgtgacgc ggcaagcgaa
     2521 cggtgtgcgg acggccggtg ggcgacaaac cccatcgtgc cgtccgcaag cgtttctgga
     2581 cctcactgtt ccggcacgtt ccgttccagt tccgccagcc aggcattcgc attgccgtct
     2641 gatggcgcac gccagtcgcc gcgaggagac agcgacccgc ccgcgctgac cttcggtccg
     2701 ttcggcatcg ccgaacgctt gaactggctg aatgcgaaga accgacgcac gaacacttcc
     2761 aaccacctgc ggatcgtcgc caggtcatag gcgccgcgcc gctccggcgg aaaaccagga
     2821 ggccagtcgc ccttcgcggc gtcgccccag gcattcagtg ccaggaacgc gatcttggac
     2881 ggcacgcagc catggcgcag cgtataatag agattgaagt cctgcagcgc gtagggtccg
     2941 atgaaggcct cggtgctctg cggcgtctcg ccctccttcg tcgggaccag ttccggcgtg
     3001 atctcagtgg caaggatcgc atcgagcgtc gccgatactt ccggcgcgaa ctgcttcgag
     3061 ctgatcaccc accggatcag gtgctggatc agcgtcttcg gcacgccggc gttgacattg
     3121 taatgcgcca tctggtcgcc gacgccgtac gtgcaccagc cgagcgcaag ctccgacaga
     3181 tcacccgtac cgatcacgat gccattgtgg tggttcgcca gccggaacag gtaatcggtg
     3241 cgcaggccgg cctgcacgtt ctcgaaggtg aggtcgtagg ccggctcgcc gcgcgcgaac
     3301 ggatgaccga tgtcggccag catccgttca gcggcagggc ggatatccag ctcctgccag
     3361 gtcacgccga gcgcctgcat caggcggacc gcgttggcct tggtatgagc cgaggtggcg
     3421 aagccaggca ttgtatacgc aaggatattg gcacgtggca ggctgagccg atcgagcgcc
     3481 tgggcacaga caatcagggc gtgggtagag tcgagcccgc cggatacgcc gatcaccacg
     3541 tgtttcacac cgatggcacg cagccgctgc gtcaggccgg aaacctggat gttgtatgcc
     3601 tcgtagcagt cctgctcgag gcggcccgga tcggacggaa cgaagggata ccgctcgacg
     3661 cggcgttcga ggcctatgtc gccgccgggc ggatcgacac ggaacgtgat gcgccggaac
     3721 ggggtagcat ggcggtggcg attggcgtcg aagctgccca tctgcatgcg ttcgtggcac
     3781 agcagatcga ggtcgatgtc ggcgatcgcg acctgactgg aagatgggaa gcgttccgtt
     3841 tccgccagag tggtaccatt ctcgaagatc gaggcctggc catcccaggc gagatccgtg
     3901 gtggattcac ccgcccccgc cgcggcgtat agataagcgg ctacacagcg cgcggattgc
     3961 gaagcgcaca acaggcggcg tgtgtctgcc ttggcaatgg tgatgttgct ggcagacagg
     4021 ttggcgagca cgttcgcgcc agccagcgcg gcggtcgaac tgggtgggac agggatccag
     4081 acgtcctcac agatttccgc gtggacgatc aggccgcgaa tgtcctcagc ctggaacagc
     4141 aggtcggtgc cgaacggagc tgtggctccg gcgatcgtga tctcgccgtt cgtcgcaggg
     4201 tcaccgacga cgaagtgccg cggctcgtag aactcccgat agttggggag gtgcatcttc
     4261 ggcaccacgc cgagcagacg gcctcgatgg atcgcaatcg cgctgttgta gagcgcgccg
     4321 gcatggcgca gcggcgctcc taccaggagc agaggcagca aattcgttga ggcttccacc
     4381 agggttccaa tcgctgcttc caccgcgtcc agcaaagtga actgctgcag cagatcgctg
     4441 atcgcgtaac cagacacaca aagttccgga aacaccgcca gcgccacgcc gcgttgatcg
     4501 caggcgcggg cgacatccag gatttccgcc gcgttcgcca tcggatctgc cagcgtgcaa
     4561 cgcgtcgtgc acgccgcgac gcgggcaaaa ccatggcgat agatggagcc gaactcttgc
     4621 atgcctgcaa tatggtgcat cgccgcacac cagtcacctt gttcaggcat tcgcgaagca
     4681 agcacctgtg agcgggtgaa tcgagagtgc ccccctatct ttccgcccga tcaaccagtt
     4741 cgtacagggg caaaactcag tcatgttgag attggcattg ttcttcctgg tggtgtcgct
     4801 ggtcgcggcg ctgttcgggt ttggcggcat atccgccgcc tcggcggata tcgcgcgcat
     4861 cctgttcttc attgccatcg tcgtcttcct ggtcctgttg gtcgcaggac tgatggccgg
     4921 gagtacgtta ttctagctac catctcacgg atgtctcatc gcgcgcggtt ccggagaccg
     4981 cgcgcgtccg tgaggtaatg gagacggcct cgcgcttgcc gcgcgcggac cgacccgccg
     5041 tatcccttct cgagccgcga ccggctatcg ggctggtttc ctgttcaacg ccgcggcagc
     5101 gcggatcagc ccctggaaag caccggcgtc aagttcgtca ccttcatgga tgtcgatggc
     5161 gcgcctcgca ttgccgtcca ggctggcgtt gaacaggcct gacgggtcct cgagcgaggc
     5221 gcccttcgca aaggtcagct tcacggcgct cttataggtc tcgccggtgc agatcatgcc
     5281 atcccgatac cagaccggaa caccgcgcca cttccactcc tccaccacgt cgggaaccgc
     5341 ctgtttgatc agtgagcgga catgggcgag cgtcttccct cgccagtctc ccagttcctt
     5401 gatcctggca tcgatcagcc gtgaggggtt ttcgctggtc ataatcggcc cgcgctaaac
     5461 caccttgccc gccagatgcg tcttgtaggc cggccgctcg agcagcctgt cgtaccaggc
     5521 gcgcagatgc gggacctcag gccgatcgaa ctccatgctg aaccagcggt ggagatgcgg
     5581 gccccagggg atgtcggcga gcgtcagatc gtctccggcc agatagggat gcttggccag
     5641 ctcggctgcc accatccccc acgccttcgc ggtatcctgc acaccctgca tgatcgcctt
     5701 catgtcgcgc tgatccggcg gcgtgcgcac cagcccccag aacaccacgc ccatcggccg
     5761 gttcagcacc gtctgctgcg catccatcca atggtcgata ttggcgcgtg catgcggatc
     5821 ctgcggccac agcttcgaat gcggcgagtg cgcgtggcag agataacggc agatcgcgtt
     5881 gctctcccac agcgtgaagt catcttcctg cagtgtcggc accaggccgg tggggttcat
     5941 ggcgcgatat tccgcggtgt cggtcttgcc gaacgaaccg ccgacatcaa ggcgttcgta
     6001 cgggagctgg agttcctcca gtgtccagat caccttcatc acgttgctcg acgtggcgcg
     6061 accccacagt atccggcgca ttcccgaccc tcccgacctg acagcactgt aaccctatgc
     6121 cgcttgcacc attgctggaa gcggggcaca tcgccgcgca tggtaatacc gtcgatgcgc
     6181 agcctcgcgc tgctgagcgc cattgccgcg ggcttcgcgc tgggattggc ggaggcgtcg
     6241 gagcactggg gtggcctggt gccctgcgcc ctctgcctgg tggaacgctg gccataccgc
     6301 gtggcgatcg ccctcagcct gatcgcattc gccgtgccgc gccagctcgc ccgctggctg
     6361 ctggtattga ccgtattgac gatcctggtg ggcgccgcct tcgcctttgt ccatgtcggg
     6421 gtcgagctgc atttctggcc cagcccgctg ccggaatgcg ccgcgccgtc gctgggctcc
     6481 ggctcgatcg ccgagcggct ggcgcagatg ccggcacgac ccgccaagcc atgtgacgcg
     6541 ccaaccttcc tgatcccggg catacccttg tcgatggcgg cgatgaacct gatctatgcg
     6601 atggttttcg tggcggcact gaccattggc ctgtggcgga aatggaggac tgcatgaacc
     6661 ttcccggcga tcgttcgcac gaatcacaag tggcccggat cgttcgcgtg gaccatgccg
     6721 gcgaatatgg cgcggcgcgc atctacgcag gccagcttgc cgtcctgcga aggcgagagt
     6781 cggcgccggt gctgcgcgag atgcaggcgc aggaacagga acatctggat cgctttgccg
     6841 aactgatcca tcgccggcgc gtgcgtccca cggcgatgct gccattgtgg catctggcag
     6901 gtttcgcatt gggtgcggcg accgcagcgc tgggtgagcg cgcagccatg gcgtgcacag
     6961 tcgccgtgga agaggcgatc gacgcacatt atgcgagcca gatcgatgca ctggatgaca
     7021 gcgaagcgga gctgcggggc acgctcacga agttccgcgg cgaagagttg cagcatcgcg
     7081 acacggcatt ggcacatggc gcggagcagg cgcccgggta tcggctgctg tcggccgcga
     7141 tcaaggccgg atgccgggta gcgatccgta tcagcgaacg ggtctagctg ctcgcgttca
     7201 ccgcgtctta accggacttt gccaaagccg tgcgatgctg cgcgcgatcg tcctgttggc
     7261 cttgctgctc acacccggct ttgccacggc cgaagagctg aagttcgcga cgtggaacct
     7321 ggaatggctg accgtccgtt cgcgtgagag tgccgacctg ccgcgcgatg cgcatccgaa
     7381 gcgggcagag gacatcgaga cactcaatca ctatgcggcc gacctgaacg ccgatgtgat
     7441 cgccattgag gaagtggatg gcgccgccgt cgcggcacgc gtgttccctg cggacaagta
     7501 ctcgatccat atgagccagg atcaggacgt gcagcgcgtt ggcgtggtcg tacgccgcgg
     7561 catccgttac agcgtcaatc ccgacctcac cgacctgaac atcaatcgtc atctgcgcag
     7621 tggggtggat gtaacgttgc ggctggatcc cgcaccgcta cggatccttg ccgtgcacct
     7681 gaagaccggt tgtctcgacc ggccactgag caatcgcggc agtggcgact gcaccgagtt
     7741 gcggcagcag gcaacggtgc tggcaggctg gatcaaggca cgacagtctg aaggcgcggc
     7801 gttcattgta ttgggcgatt tcaatcgcca tatggacgag cgggataagt tttggcctgc
     7861 gctggcgcgc gcagctccac tgatccgcgc aacggcaggg cacagcagcc cgtgctgggg
     7921 cggcgaagcc ttcatcgacc atatcatcct gggtggtgcc gcacgcgact ggctcgtgcc
     7981 ggactcgttg cgcgtgctga cctatcgtga gactggaccc gaatggaagg atcggctttc
     8041 cgatcattgt ccggtgtcgg tgcgcttgcg cgtgcctgat ccggcgcgtt gaatgcgcgc
     8101 tccacgggag gagcctgcgg catgaagatc accggcatca cgacaaagga ataccgctgg
     8161 ccgcgcaaca agccgatcgc caacggcaag cacacctata cgcacgtgac cttcgcggtg
     8221 gtggaaatcg agacgagcga cggcatcacc ggcatcggtc ttggcggcgg gggcgagatc
     8281 gagcgcgcga cggtcgcgag gcttgcgccg atgctgatcg gacaggatcc ggtgaatgtg
     8341 gagcggctct ggcatcaaat gtgggagccg aagctgatcg ggcgccgcgg cctgacaaca
     8401 cgggcgattg gcgcgatcga tatcgggcta tgggacattc gcgccaagct tgccgggctg
     8461 ccgctctacc ggctgctcgg cggctttcgg gatcgggtgc cgacatatat cgccggcggc
     8521 tattacgagg aaggcaaagg cttgcgagaa ctggcgcgcg agatggaaga caacgtccgc
     8581 atgggcgcca aggccgtgaa aatgaaggtg ggcgccgtgt cgatcggcga agatgtggcg
     8641 cggatccgtg tcgcgcgcga ggcagtgggc ccggacgtga agctgatggt ggatgccaat
     8701 tgcgcctatc gatattacga ggccatccag ttggcgaagc gcatcgagga gtatgacatc
     8761 ttctggttcg aagaaccggt gatgcccgac gactatgacg ggcatcgcaa gctggcccag
     8821 gcgaccagca ttccgatcgc aaccggggag aatgaatata cccgctacgg cttccgggat
     8881 ctgatcaagc aggaatgcgc ggcgatcctg aacgcggatg cgaaagtgct gggcggcgtg
     8941 accgagttca tgaaggtggc ggcgctggcg caggcgaatg acctcgatat tgcgccgcat
     9001 ggctcccagg acatccatgt gcatctggtc gcggcgatct ccaacgggtt gatcctggag
     9061 ttctatcgcg acacggtgga tccgatgtgg ggacgcgtgt atcgacacac cctgcggctg
     9121 aatgacgacg gcaccgtcag cccgcctgac gtgcctggca tcggcgcgga tccaaactat
     9181 gaggggctgg cggaattccg cgtggcctga cccggcgtca tggccgcgct cgtcgcggcc
     9241 acccacgtct tcaacaccaa aggaacacga agcatggcca gaattcttgt catccacggc
     9301 gccggcatga acatgcgcgg caaggtgcag actgaaatct tcggtccgat gacattgccc
     9361 gagtacgacg agcgcattcg cagctacgcg gccgagcttg gcgtcgagat cgagatcttc
     9421 cattcgaaca tcgaaggcga cgtgatcaac aagctgtacg aggcgaacga caaggggttt
     9481 gatgccgcgc tgttcaatcc ggccggcttc tcccgcggct atccggcgct ggttgcggcg
     9541 atcaaccagg tgaagttccc gacgatcgaa ctgcatatct ccaatccggt gcggcgcggc
     9601 ccggtatcgg acaccgcgac cgtcagccag gggatggtga ccgggttcgg catcgccggc
     9661 tactacctgg cgctgcgagg attgcgcgat acgctgaaga agtagcggtt cagcctttgg
     9721 gcgctgcagc gtacccaaag aaagagcgca gttatcaaac acagatggaa acagatggtg
     9781 cccatcgagg cacgcactgc ttgacgcccg cacccggcct ctgctaatga acgaacggtc
     9841 gttcagatcc tatcgcaccg atgaccagca aacgcgacct gatcaagtct gaagcaaccc
     9901 gtctcttcgt cgcccatggc gtcgacgcag tctcggtccg cgacatcgcc gcggcctgcg
     9961 agatgaaagc ctccaacctt tatgcccact tcgcatcgaa ggacgccatg gtcgccgagc
    10021 tgttccgcga aggctacgcc gaatacggcg ccgttctcgc tgaggccgct gccggcgccg
    10081 aacccttcca agtccgcctg gaccgcatga tccgggcgat ctgccgcctg cacgatgagg
    10141 acgtcgtccg cttccgcttt ctgatcctga cccagcatgc gttcctgcgc gaggtgccgc
    10201 atgacgacca caaccctgtc gagatcatct gccgcgcggt cgccgccgcc atggaagccg
    10261 gcgagatccc cagccgtgag ccggaactga tggcgatggc cgtcatcggc atcatcgtgc
    10321 agcccgccac tgcctgcctc tacggccgcc tggacggtgg cctggtggag cgcgccggcg
    10381 agatcgtcgc gattacctgg cgcgcgttgt cgtaacatcc gcctcgaaag gacaccgcca
    10441 tgtccgcctc gccgctcgga ttgctgaaca cccgccgcat gtggccgctg gcactaaccc
    10501 agtcctgcgg cgcgctgaac gacaacctgg tgaagaacgc catggtcgtg ctggcgctgt
    10561 tccagctcgg catcggcggc acaggacttt cggcgctcgc cggagcgctg ttcatcgcac
    10621 cctacatcct gttctcggcc accgccggga agctggccga ccggttcagc aagccccgcc
    10681 tcatcgtcgt ctacaagctg gccgaggtgg cgctgatgtg tggtgccgcg gtcaccttcc
    10741 tgaccgagag cgtcaccggg ctgctgttgc tgctggcagg cctcgggctc caggcaacgc
    10801 tgttcggtcc ggtgaaatat gggttgctgc ccgagcacct tgtcgagcac gagctggtgg
    10861 ccggcaacgg gatcatcgag gcgacgacgt tcctttccat cgtcatcggc accgtcgcag
    10921 gcggcgcgct ggtgcagttg ccgcagggca ccgtgtgggt cggcgctacc ggtatagcgc
    10981 tggcgctggt gggactcttc ggcgcctggc gtattccgcc gtcgcccgcc gccgacccct
    11041 cgctgcggat ttcagccaat gtgctcacgg agacgtggcg ggtcacgcgc cattccgcgt
    11101 cgatccgcgc catctggctg tcgatcctcg gcctctcgtg gttctggacg atgggtgcga
    11161 cgctgatgac cgagttcccc gtcgtcgcgc gcgacacgct gcacgccgac ggcaccgtgc
    11221 tgaccctgct gctgaccgtg ttcgcgattg gcgtcggcgt tggctccatc cagtgcgcac
    11281 ggctgctgca tggcgacgta tcgccgcggc tggtgccgtt cgcagcactc ggcatttcgc
    11341 tcttctgctg ggacttcgct tccgcgtcag gctctgccgg cagcatcgcc aatgcctggg
    11401 tcgcgctgtc tggtttcgca ggctggcgga tgatcgccga tctgttcctg ctggccgcat
    11461 gtggcggcgt gttctcggtg ccgctgtacg cgatcatcca ggatgcctcg gcgccggccg
    11521 agcgctcgcg gatgatcgcc gccaacaaca tcatgaacgc cctgttcatg gtggcgggcg
    11581 cgggcgccac ggcggggctg gcggcgtgcg ggctgaactc cccgcaggtg ctgcatgtcg
    11641 ccgccgtcgc caatctgctg gtcgcgatct ggatcgtgcg catcctgccg caggagttct
    11701 atcgcgcgat ccttcgctgg tatttcgacc gcttccacca tgtgcgggtc accgggctgg
    11761 agacctataa ggccgctggc gaccgcgtgg tgatcgtggc caatcaccaa tcctatctcg
    11821 atgcactcct gatcgcggcc tatgtgccgg atagtccaac ctttgcgatc cacacctcgc
    11881 aggcagcgaa gtggtacttc aagccattcc tgcgcgtggt ggagacattt ccggtcgatg
    11941 tgcagtcgcc ctacgcggtg aagcgcatgg tcgaggcggt acgcgaccag ggccgcaagc
    12001 tgatgatctt ccccgagggc cgcatgacgc gcaccggtgc gttgatgaag gtctacgaag
    12061 gcgcggcact ggtcgcagac aagtcgcacg ccaagatcgt gccgatcagc atcgagggcc
    12121 tgcagttcag ccgtctcaac cgcatggggg acaagctgca tcgccggtgg ttcccccgcc
    12181 tgcggctgaa catcctgccg ccggttacgc tggcacctcc gggggctgag caactgacgc
    12241 atcgccagcg ccgcgaagtg attggccgcg ccttgcagga cgtgatggtc aatgcggtgt
    12301 tccgctcgaa ggataccggc aaaagcctgt tcgcggcctt gctcgacgcg cgcgatgcgc
    12361 acggtgccca cacgcccatc atcgaggaca tcgagcgcat tcccatcagc tacgatcgcc
    12421 tcgttgtcgg ttcggcggcg ttggggcgcg cactgaccgg cctgaccgct gaatcgcatg
    12481 tcgcattgat gatgccgaac gccgcggcaa cggtggtggc gttcatggga ctccaggcat
    12541 atggccgtgt gccttgcatg ctgaacatct ccgccggtgc tgcgtcgatg ctgtcagcct
    12601 gtcgcgcggc aggcgtgcgc agcgtgatca gcagccgcac attcgtggaa aaggggcgcc
    12661 tctccaaggt ggttgaacgg atggaagccg aggtgcgctt catctggctg gaggatgtgc
    12721 gcgctgccat cgggttgcgc gcgaagctgc gcgccaagtg ggatgcgcgc cgcgcccggc
    12781 ggctgcccgg cgcgtcgacg gaccctgact cgccggcggt cgtgctgttc accagcggtt
    12841 ccgaaggcgc ccccaagggc gtgacgttga gccatcggaa cctcatcgcg aattgcgcgc
    12901 agcttgcctc ggtgatcgat ttccacgggg gcgacatcgt cttcaatgcg atgccgatgt
    12961 ttcacgcgtt cgggctgacc ggagggacga tcctgccgct ggtctccggc gtgcgcacgt
    13021 tccattaccc gacccccctg cactatcgcg tggtgccggc gttgatctac gacaccgacg
    13081 cgacaatctg cttcgggacc gatacgttcc tgaacggctg ggcgcgatac gcgcatccct
    13141 acgatttcta cgccatgcgc tacatcttcg ccggcgccga gaagctgcgc gaggagacga
    13201 agcatctgtt cgctgatcga tttggcgcac ggattctgga agggtatggc gcgaccgaga
    13261 ccgcgccggt gctggccatc aacactgcga tgcacagccg caacaatacc gtggggcggt
    13321 tcctccctgg aatcgaatgg cggctggaac cggtggccgg catcgagcaa ggcgggcgtc
    13381 tatacgtgca cggacccaac gtgatgttgg gctacctgcg cgacactgcg cccggcgtgc
    13441 tggagccgcc gccaggcggc tggtacgaca ccggcgatat tgtcgcgatc gatgcggccg
    13501 gtttcatcac catcctcggg cgcgccaagc gcttcgcgaa gattggcggc gagatggtgt
    13561 cgatgaccgc agccgagatg ctgatcgcag cgctttggcc aaatgcacaa catgcggtga
    13621 tcaacctccc cgacgcacgc aagggcgagt acctgctact ggtgacgaca cgccccaacg
    13681 ccgaaccccg cgacatcctg acttccgcgc gagagcgcgg catcccggag atcatggtgc
    13741 cccgttcgat catgcaggtg ccggcggtgc cgctgctcgg caccggcaag gtggattacc
    13801 cggccgtgca gcggatggtc gaagccgcac ccgcaccaca accgcaggag acggtgcccg
    13861 cctgaccttc gccccgcctc gcgccctgtc ccaagcatcg ggcatagcgg catcatacaa
    13921 agacagcgca tccacgcagg accgcaggcc gggtcgcatt gcgtaatgac ccaccttgag
    13981 tgtcatccag aggtagcatg ccagagctga accggctccc cttgcgatct gtcctgggat
    14041 cgctggtgct gatcgcagtc gtcattggtg gaggggccgc ggctttcgcc tataccgccg
    14101 ggtggttttc gccgcagcgc ctttccggcg cccgattggt ggcggctttc acaccgccgg
    14161 gcgataagcc gccgctcggc catcggcgca accacgccaa gggcatttgc ttcaccggca
    14221 cgttcgaagc gaatggcgaa ggagaggcgc tctcctacgc ccaggtgttc tctcatggcg
    14281 cgtatcccgc gctgggacgg ttcaatctcg gcacacctca tcccgacgca ccggactcaa
    14341 cggtgcgggt gcgcggcatg gggctcagca tcacgacgcc cgacgggcag gtgtggcgcg
    14401 ccgcgatgat caatgcgccg gttttcccgg tatccacgcc gcagcaattc tatgcgcttc
    14461 tgatggcatc gggcagcaag gacccgaagg cgatgccgga cttcatcgcc gcgaaccccg
    14521 agttcgcccc atttggcgcc tgggccacga aaggcacgtg gaccggcagc tatgccgagg
    14581 agcgctacaa cagcctggac agcttcgtct tcaccaacag tatcggcgtg gatcatgcag
    14641 tgcgttggtc actgcttccc gaggctcagg tcgcaccact gccgccggat gaacttgcca
    14701 agcgcggatc agactatctg gagcaggaga tcgtgcagcg ggtacaggcc gcaccgcagc
    14761 gctggaccat gacggtgacg gtcgccgcct cggttgatcc gacctccgat ccatccaagg
    14821 cgtggcctga tgatcgccgc acgataacgg tcggaacgct ggtggtgcag cggatcgagg
    14881 ccgaggccga cggtccttgc cgcgacgtca acttcgatcc gaccatcctg ccggcgggaa
    14941 tgcgcacctc gaacgatccg ttccccgcgg cgcggtcggc tgcgtatgcg aaatcctatg
    15001 acctgcgcac ggcggaatct gccgactatc cgcacacccc gacggaggcc aagccgtgaa
    15061 ccacgttgag ccacgcttca ccgccgtgca gcgcctgctg cattgggtga tggcggtgtg
    15121 catcctggca atgctgctca tcggcgtcgg gatggagtcg acggtgatgc cgaaatacct
    15181 gacgcttgtc gcaatccata aaccgttggg catcgcaata ttggtgctgg cgctgttgcg
    15241 cctcgcggag cggctgcgca tcggcacccc gccgctgcca ttggacctgc ccgagccgat
    15301 gaagctcgcg gcgcatctgt cacactatgc gctctatatc ctgatgatcg gcatgccgct
    15361 gctcggctgg gcgatgctgt cggcggcaga gtatcccgtc gtgctgtatg gcagcgtgca
    15421 tctgccgtcg atcgtgccgc aaagcgacag tctgcacgcc ctgttatggg atgcgcacta
    15481 ctacctggcc ttcgcgctgt tcgcgctgat tttgatgcac atcgcggcgg cactgttcca
    15541 cctgctggta cggcgtgacg gggtgttcca gcagatggcg cctatgcacc tgcacgaaga
    15601 ggacccggag gagattgtcg tcgccgaata aaagacgtcg gcaccggttg accacgcggc
    15661 catcgcgttt ggccggatcc gccgggagcc aattcggcac ttgcgtgcca acagcatata
    15721 agcatatgtt tatgggtcgg tggtcccgcg cgagcgggtc aatagggaac gccgtgcgct
    15781 ccggctggac taaacgtccg ggagcaagtc ggcggctgcc cccgcaactg tcagcggcga
    15841 gccgctgacc cgtctgccct ggcaggcagg ccactgaagc ccggacgcgg gtttcgggaa
    15901 ggcagggtca gcgacggtga accgcgagcc aggagacctg ccatcgtcgc accaaccaac
    15961 gctgccgggc ggggtgcacc gggagctgga gacgacgcat gaccatcgcc agcaatctcg
    16021 gctttccgcg tatcggcgcg cggcgcgagc tgaagaccgc cctcgaacgc ttctggtccg
    16081 gcatgattga tgaggccgca ctgaacgcca ctgcgcagga actgcgcgcg cgccattggc
    16141 gcacccagct cggcagccgt atcagccatg tgccgtcagg cgatttttcg ctctacgacc
    16201 acgtgctgga caccgcctgt atggtgggtg ccatcccgcc ggggtacggc tggtcagagg
    16261 ggccagtatc gctcaacacc tatttctcac tggcacgcgg tgcgcgtggc gagaccggcc
    16321 tgccggcgct tgagatgacc aagtggttcg ataccaacta ccattacctg gtgccccggc
    16381 tgcaccccgc gcagcggttc acgctgaccg ggaacaggcc tctggcgcag ttcatcgagg
    16441 ctgccgcgct ccccatgcgc acaaggcccg tgctgcttgg tcctgtcagc ttcctgttgc
    16501 tggcgaagac cattgacggg tccggcgcgc tcgaactgct gccggcgctg ttgccagtgt
    16561 atacggccat cctgaatgag ctggccgacg ccggagctat ctgggtgcag atcgatgaac
    16621 ccgcgctggc tctcgacctt ggggccaagg cgaaggccgc gttcaacacg gcctatgccg
    16681 cgcttgccgc cgcgccagcg ccgaacatgc tggtcgccag ctatttcggt gcactggccg
    16741 acaacctgcc ggtggtggcc gcgctgcccg tcgcaggcct gcatctcgac ctggttcgtg
    16801 gcggacaccg gctggatgaa gcgcttaccg cgataccagt ggatcgctgg ctgtcactag
    16861 gcctggtcga cgggcgcaat gtttggcgta cggatctgcg gtctgcgctg gcaacattgc
    16921 agcagatcgc gacggcacgc ggcgcgcagc ggctgatggt ggcgccgtcg tgcagcctgc
    16981 tgcacgttcc cgtcgatctg gcgcaggaga gcaagctcga ccccgaggtg aagagctggc
    17041 tcgccttcgc cacgcagaag ctggccgagg tggctgcgct ggcgcgtggc cttgacgaag
    17101 gtgaggcagc gatcgccagg gaactggaag caagcgatgc tgcgcaacgt tcgcgccatg
    17161 gcagcagtcg cgtgcaccgg aacgaagtag ccgagcgtct ggccgccacc acgccggcga
    17221 tggaacggcg cgccagtgcg tatccacagc gccgcaccgg gcaacaggca aggctgaaac
    17281 tgccgccatt cccgaccacg acgatcggct cgtttccgca gacgagcgat gtacgccagg
    17341 cccgcgcgga ccgtggccgc ggcaacgcga ccgaggctga gtacgcggcc agcatcgagc
    17401 actggatcga cgacgcagtg cagtggcagg agcgtatcgg cctcgacgtg ctggtgcatg
    17461 gcgagttcga gcgcaacgac atggtgaagt atttcggcga gcagctcgat ggctacgcct
    17521 tcacccagca cggctgggtc caatcctacg gcagccgctg cgtcgcgccg ccgatcatct
    17581 ggggcgatgt agcacggcca gggccgatga cagtccgctg gtcgtcgtat gcacaatcgc
    17641 gtacacgacg accaatgaag ggaatgctga cgggtccggt caccatgctg caatggtcct
    17701 ttgtgcgcga cgatatcccg cgcgagaccg tctgccggca gatcgcgctg gcaattcgcg
    17761 acgaggtgat ggacctggag gccgccggca tcccgatcat ccaggtcgat gaacctgcgt
    17821 tccgcgaggg gctgccgctg cggcacgctg aatgggcggc gtatctcggc tgggccgttg
    17881 cctgcttccg ccttgcgacg gccggcgtgg cggacgcgac cagtatacac acacatatgt
    17941 gctacagtga gttcaacgac atcatgccgg cgatcgcagc gatggatgcc gacgcaatct
    18001 cgatcgagac gacgcgcagc cggatggagc tgttgaacgc gttcggcgcg cagggtgcct
    18061 atccggcgga gatcgggccg ggcatttggg acatccacag tccccgtgtg ccggatgccg
    18121 ccgagatggc ggaactgctc ggcctggcgc gccagcggct ggctgactgg cagatctggg
    18181 tcaacccgga ttgcggcctg aagaccagga aatgggagga agtgcgccct gccctggaaa
    18241 acctggtcgc ggcggcgcgg cagatgcgcg atgcaatggc gggcggctga gcaggccccc
    18301 gctatcgcgg ctgcatccgc aaggcgccat ccagccggat cgtttcgccg ttgaggaagc
    18361 gattggtggc gatgtgctcg acgagcgcgg cgtattcgcc gggctcgccg aggcgctgcg
    18421 ggaaaggcac cgcttcgccg agtgcgcgct gggtctcttc cggcagcgcc ccgaccatcg
    18481 gcgtgtggaa caagccgggc gcgatggtca ccacgcggat gccgaagcgc gcgagttcgc
    18541 gggcagcggg gagcgtcatg ccgacgacac ccgccttcga ggccgcatag gcgggctgac
    18601 ctacctgacc ttcgtaggcg gcgatcgaag cggtgttgac gatgacgccg cgctcgccgt
    18661 cgtccatcgg gtcgagcgtc gtcatatcgg ccgcggcgag acgcaacagg ttgaatgagc
    18721 cgatcaggtt gatgcggatc acgcgctcga aggcggcaag cggcatcggc ccttcccgcc
    18781 cgacgatgcg gcctgcggta ccgacgcccg cgcagttgac caggatgcgg gcgggaccgt
    18841 gcatctcgcg cgctttcgcc atggcggctt cggcggaggc ggcgtcggcc acgtcgcagg
    18901 cgatgccgtc cgcgcctggg ttgatatcga ggatcgcgac gcggcagccg gctcgccgca
    18961 gccgttcggc ggtcgccgca ccaagaccag aggcaccgcc ggtgacgata gcggcctgac
    19021 cgtcgagacg cattgcaggt tccctcgcaa gatggcaggt ctcagtctgg ctcagcgccg
    19081 catcgaagcg ccagaccaag ggagtgaaac gccgtgcatt tccgtatcac cgcgctgttg
    19141 tgcgcgctgc tgttgtccat cgcgggcgcg caggcgcagg acaagacggt tcagctgaaa
    19201 ctgtcgagct gggtcccccc ggcgcatcca ctgaacccat cgctgcaggc ctgggcagct
    19261 gacatcgaaa aggcatccgg cggcaccatc aaggccacgc tgttcccgtc ggagcagttg
    19321 ggcaaggcga tggaccatta cgacatggcg cgcgacggga tctctgactt ctcctacgtc
    19381 aacccgggtt atcagcccgg gcgattcccg atcattgccg cgggcgagct gccgttcttg
    19441 gtcgccaacg cgacaggtgg ttcggcagct ttggacgcct ggtaccgcaa atacgcaccc
    19501 accgagatga aggacgtgca cttctgcttc gccttcatgc tcgatccggg cacgttccat
    19561 tcgcgcagca ggaagatcgt ggtgccggaa gacatccgtg gcgagaaggt gcgcccggcg
    19621 gatgccacga tgggcgcctt cgtgacactg ctcggcggca cgaacgtgca ggcatcggcc
    19681 ccggaggcac gcgaagtgct ggagcgcggc gtggccgatg cgatcacctt tccctggcac
    19741 tccaccatcc tgttcgggct cgacaaagtg gtgaagtact caatggacat gccgttcttc
    19801 agcacgacct tcgtgtgggt gatgaacaag gcgaaatacg actcgatgtc gcccgcacag
    19861 aaatcggtga tcgacaacca ttgcacgacg gaatgggcag agaaggtcgt gacaccctgg
    19921 gccaactggg aacgtgcggg acgcgagctg ctgatcgctg aaccaggcca cgacgtctac
    19981 aagctgaccc ctgagcagca ggcagcctgg cgcaaggccg cggagcctct gaaggcgaaa
    20041 tgggcggcgc aggtgaagga cgccgacaag gtatatggcg agttggtgga cgagctgaag
    20101 aagcgcggcg cactggcgga atgaccgcga tcgcggtcat gccgggctag caccatgagc
    20161 gatctggaac gcgagttcgc ccggaaggcg gcggcagagg gcgccgcccg cagccggatg
    20221 gaccgattca tcgacgccat cgagctgttg gccgccatct tcgtgggcat cgttgcggcc
    20281 gacgtattca tctcggtgct gctgcgctac ttcttcgcgg tctcgatccc tgacagctac
    20341 gacttcggca agatgctgct gggcattctc atcttctggg gtatcgcggc gaccagctat
    20401 cgcggcacgc atatcacggt ggatctggtc tgggcggcgg caggaccaag gggcaagcgg
    20461 ctgatcgata tcttcgccac gctggtgctg ctgttcgtgg tgtcggtgca gacggcgatg
    20521 ctgttcgaca aagtgcggca gacctacgag gagaacgtcc tcacctttga cctgcacctg
    20581 ccgacgtggc cgttcgcgct gatcgcctgg ctcggtgacg tatcagcggt gctgctgatc
    20641 gccatccgca cctaccggct gatcttccac ccggaagacg ttgccgacgc aggcgtcgtc
    20701 aggacggtcg agtgatcgag ctcggcagcg acgccgtcgc catcatcggc ttcatcgtgc
    20761 tgttcgcact gatgctgctg cgcgtgccgg tgggcatggc gatgggcctg gtgggcgtca
    20821 ccggctttgg ctatctggtt ggcggcatgc cggcgctgaa gatggtcggg cagacctcga
    20881 tgcgcacggt caccgattat acgttcggcg tgatcccgat gttcctgctg atgggcgcgt
    20941 tcgtctcgcg ctccggcatg agccgcgagc tgttccaggc ggccaacaag ttcgtcggcc
    21001 atctgcgcgg tggactgggc atcgccacca tcgcggcgtg cgcggggttt gctgcgatca
    21061 gcggttcgtc tgtggcaacg gcggcgacat tctccaccgt cgcgtatccg gagatgcggc
    21121 agtaccggta tccgcagtcg ttctccactg gggtgattgc cgcgggcggc actctcgggg
    21181 cgatgtttcc accttccatc gtgctcgcgg tctacgggct gatcacgcag caggacatcg
    21241 gcaagctgtt catggccggg gttctgccgg gcctgcttgc agtgtcgatg tacatggcga
    21301 cgatcggtgt catcggcgca gtgcggccgg cgtatctgcc ggcggcgcca cgcagttcgt
    21361 ggcgcgagcg gttgcaggga ctgcgcgaag tgtgggcgcc gctgttgctg ttcgcctttg
    21421 tgatcggcgg cctgtatggc ggcctgttca caccaaccga ggcaggtggt gtcggcgcag
    21481 gcggcgcgtt cctcatcggc atcgcgcgtg gccgcctgtc gcggaccgat atcctggagt
    21541 cgctgctgca ggcgacgcgc accgcggcgg cggtgttcac catcctgatc ggcgcgatcc
    21601 tgttcggcta tttcctgacg gtgacgcaga cgccgcagaa ggtgaccgag tttgtcaccg
    21661 ggctgggatt cggccgctac ggcgtgctgg tgctgatcat gctgatgtat ctggcactcg
    21721 gctgcctgat ggatgcgatg gcgatgatca tcctgacggt gctgatcatc ttcccggtga
    21781 tcaccacact ggggttcgac ccggtgtggt tcggcgtgat catcgtcatg acggtcgagc
    21841 ttgggctgat tcatccaccg gtgggaatga acgtgttcgt catcaagacc gtggtgaagg
    21901 acgttgcctt ctccacgatc ttcatcggcg tgatcccgtt cgtgatcacc gacatcattc
    21961 ggctggcgat cctgatcgcg ttcccagcgc tggcgatctg gctgccgag
//
LOCUS       DATGEM010000597         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_268508, whole genome shotgun sequence.
ACCESSION   DATGEM010000597 DATGEM010000000
VERSION     DATGEM010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268508"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VKQ27_18340"
     CDS             complement(<1..301)
                     /locus_tag="VKQ27_18340"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY90948.1"
                     /translation="MSAPIAGLAALNFFLADVQGGLGPFLATWLAAIHWSPGQIGVVM
                     TVGALVGLVCNSPAGVLIDRTQRYRLWLALSSSAIVVGTLAMLPARGFVPVLVAQV"
     gene            complement(359..1168)
                     /locus_tag="VKQ27_18345"
     CDS             complement(359..1168)
                     /locus_tag="VKQ27_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLY90949.1"
                     /translation="MAGVILAVLDHPAATQALLDVARQLAEQSGAARINALVVRAPPE
                     TLISPSEEVLTEQREEYLHTIEASRAGKVREVFDAWAATVPTGIKADWIDIDGIAELL
                     VEERGRRADYIVVEQPSRSDYGTSWHALRAALFATNRPVLLVPAGQARSFGQSMAIAW
                     RDDERATRAVLSALRAFPKAANRFVLAGTRGADVVLPPILAEHGVTAELHALPIGGGP
                     FGEALLRKAHQLGADVLVMGAYEHHPLRELLLGGVTRHMLSHADLPVLLQH"
     gene            complement(1170..>2275)
                     /locus_tag="VKQ27_18350"
     CDS             complement(1170..>2275)
                     /locus_tag="VKQ27_18350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY90950.1"
                     /translation="LELMQWLDRLGFHEAWIGEHHSAGWETISSPELFIAVAAERTRS
                     IRFGTGVISLPYHNPLMTANRIIQLDHHTRGRVMFGAGPGLLASDALMLGIDPSTQRD
                     RMAEALDVILRFFNGEIVTEKTDWYTFVNARAHLRPYTRPYPEVAVVSAVTPSGGRLA
                     GKYDLSMICVAATNPFGYDALAANWQIANDIAAEQGRTMDPRRLRLVGPMHIAETREK
                     AFENVKFGFERYLGYLNNNQPRFIVPPGQDAAEWFVENKFGVIGTPDDAIALIERLQA
                     KQGEFGVFLHQAHNWADWEETKRSYELYARYVMPHFSGDNIPRMESFDWCTEHRDELT
                     EKRSTAARAMFDKHEAEQRARMAARPKEGREAW"
BASE COUNT          410 a          815 c          674 g          376 t
ORIGIN      
        1 caacctgtgc cacgagcaca ggaacgaaac cacgtgccgg caacatcgcc aacgttccaa
       61 ccacgatcgc tgatgacgat agcgccaacc acagacgata ccgctgcgtc cggtcgatca
      121 gcacgcccgc gggactgttg cacaccagcc ccaccagggc accgacagtc atcacgacgc
      181 cgatttgtcc ggggctccag tgtatggcgg caagccaggt cgcgaggaac ggaccaaggc
      241 cgccctgcac atccgccaga aagaagttca gggccgcaag gccggcgatc ggcgcactca
      301 cgaatcgtac ttatcgcgcc gcggcgcttc ggcaaccgtc gacacgatca agcccgcgtc
      361 agtgctgcaa cagcaccggc agatcagcgt gcgacagcat atggcgcgtg acgccaccca
      421 gcagcagctc gcgcaatgga tggtgttcgt aggcacccat caccaacaca tccgcgccaa
      481 gttgatgtgc cttgcgcagc aaggcctcgc cgaacggtcc gccaccaatc ggcaacgcat
      541 gcagttccgc ggtcacgcca tgttcagcca ggatcggcgg tagcacgaca tcggcgcccc
      601 gcgtccccgc cagcacaaac cggttcgccg ccttcgggaa cgcgcgcagt gcggagagaa
      661 ccgcccgggt cgctcgttca tcatcgcgcc aggcgatcgc catactctga ccgaacgatc
      721 gtgcctggcc tgccggcacc agcagcaccg gccggttggt ggcgaacaac gcagcccgca
      781 atgcatgcca gctcgtgccg taatcgctgc gcgacggctg ctccaccacg atgtagtcag
      841 cgcgccggcc gcgttcctcc accagcagtt ctgcgatgcc gtcgatgtcg atccagtccg
      901 ccttaatgcc agtcggcacc gtcgctgccc acgcatcgaa tacctcacgc accttgccgg
      961 cgcgactggc ctcgatcgtg tgcagatact cctcgcgctg ttcagtcagc acttcctcgc
     1021 tcggcgagat cagcgtctcc ggcggtgcgc gcaccaccaa cgcattgatc cgcgctgcac
     1081 cgctctgctc ggcaagctgg cgcgccacat ccagcagcgc ctgcgtcgcc gccggatggt
     1141 cgagcaccgc aaggatcacg ccagccatgc taccaggcct cgcgcccctc cttcggccgc
     1201 gccgccatcc gcgcgcgctg ctccgcctcg tgcttgtcga acatcgcccg cgccgcggtg
     1261 ctgcgcttct cggtcagctc gtcccgatgc tcggtgcacc agtcgaagga ctccatgcgc
     1321 gggatgttgt cgccggagaa atgcggcatc acgtaacgtg catacagctc atatgaccgc
     1381 ttggtctctt cccagtccgc ccagttgtgg gcctggtgca ggaacacgcc gaattcgccc
     1441 tgtttcgcct gcaaccgctc gatcagcgcg atcgcatcat ccggcgtgcc gatgacgccg
     1501 aatttgttct cgacgaacca ctccgccgca tcctgccctg gcggcacgat gaaccgcggc
     1561 tggttgttgt tgaggtaacc aagatagcgc tcgaacccga acttcacgtt ctcgaaggcc
     1621 ttttcccgcg tctccgcgat gtgcatcggc ccgaccagcc gcagccgcct cgggtccatc
     1681 gtgcgcccct gctcggcggc aatgtcattg gcgatctgcc agttggccgc cagcgcgtcg
     1741 tagccgaacg gattggtcgc cgcgacgcag atcatgctga gatcatactt gccggcgagc
     1801 cgcccgcccg acggcgtcac cgccgacacc accgccacct cgggatacgg ccgtgtatac
     1861 ggtcgcagat gcgcccgcgc attgacgaag gtgtaccagt cagtcttctc cgtgacgatc
     1921 tcgccgttga aaaagcggag aatgacgtcg agcgcctcgg ccatgcggtc gcgctgcgtg
     1981 ctgggatcga tgccgagcat cagcgcatcc gacgccagca gccccggtcc cgcgccgaac
     2041 atgacgcgcc cgcgcgtgtg gtgatcgagc tggatgatgc ggttcgccgt catcagcggg
     2101 ttgtggtacg gcagcgagat cacgccggtg ccgaagcgga tcgaccgcgt tcgctcggcg
     2161 gcaacggcga tgaacagctc gggcgagctg atggtttccc agccggccga atgatgctcg
     2221 ccgatccagg cttcgtggaa gccgagacga tcgagccact gcatcagctc caggt
//
LOCUS       DATGEM010000598         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_962588, whole genome shotgun sequence.
ACCESSION   DATGEM010000598 DATGEM010000000
VERSION     DATGEM010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962588"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1531
                     /gene="ftsA"
                     /locus_tag="VKQ27_18355"
     CDS             233..1531
                     /gene="ftsA"
                     /locus_tag="VKQ27_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLY90951.1"
                     /translation="MNNMSRPVLPPPMEPDEPARTRNGRSGPFGVLDIGTTKVACLIG
                     RTESDGTLRALGFGWHRGRGVRGGSIVDLDEAERAIRACVGQAEDMADTQLRAVVVNL
                     TCGQPESRLFNVQWPVGGRAVEEHDIRRVVNEGRYRASADGREVIHALPLTFSVDQTD
                     HVADPRGLFCEELTARLHIVDASSTALRSLGACIARCDLDIGELVSAPMAAGLATLVD
                     DERELGSTVIDMGGGTTGMAVFAENQLLHTAQLPVGGVHVTNDLARLLSTPVAHAERL
                     KTLYGNVQNSPDDEREMLPVPLVGEEEHQIAKVPRSMVVNIIRPRLEETFELVKERLD
                     SSGLTRAAGTRVVLTGGACQLAGARELAGRVLNKQVRLGRPIALRGLPDSASGPAFAT
                     AAGLLAWAAGDGRTMHDVDLEAERPTGLIRRIVNFLKDRV"
     gene            1605..3224
                     /gene="ftsZ"
                     /locus_tag="VKQ27_18360"
     CDS             1605..3224
                     /gene="ftsZ"
                     /locus_tag="VKQ27_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLY90952.1"
                     /translation="MTLNLTIPQTVHTDFSPRITVIGVGGGGTNAVDNMITANLQGVE
                     FVVANTDAQQLMHSRADRRIQLGPHITQGLGAGAKPEIGKAAAEEASDELARHLDGAH
                     MVFITAGMGGGTGTGAAPVIARMARERNILTVGVVTKPFGFEGVRRSRCADQGIEELQ
                     RHVDTLIVIPNQNLFRMANERTTWKEAFKMADQVLYMGVRGVTDLMMAPGLVNLDFAD
                     IRTVMAEMGKAMMGTGEAEGENRAIRAAEAAISNPLLEDTSMSGAQGLLINITGGDDL
                     TLFEVDQAANRIREEVDEEANIIFGSAIDETLSGRIRVSVVATGIDSPAQGQGERPRL
                     VAVGGSAMPMPQPIPLPMHAAAAAVTMPAGAGLAPAPVMSAQGHPLRQPAPQLVNVAD
                     AVPDTASMMEMASMSAPQPIPHPVGPVHSMTPVHSVTASVQAVGITQPRAASGASRAS
                     LFAEPRRPAGDPAAPQVAPESTRPSLFSTVTGAFRRRQHPGAVEPAAPVRAEPVVQAV
                     ARAEPPQASVRQTAGEEVGLDIPAFLRRQSS"
     gene            3503..4432
                     /gene="lpxC"
                     /locus_tag="VKQ27_18365"
     CDS             3503..4432
                     /gene="lpxC"
                     /locus_tag="VKQ27_18365"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HLY90953.1"
                     /translation="MDGLPVSLASEDGCAELATGVQRTLKAAISCVGVGLHSGKRVTL
                     TLRPAGVNQGIVFRRTDLRRDIPARYDHVVDTRLCTVLGNGGARVSTVEHVMAALSGC
                     GIDNAIVELDGPEPPALDGSAAQFVFLIDCAGIIEQDEPRSAIEVRRVVRVTDGEAFA
                     EFRPGMFGLDMALSIDFAAAAIGRQALSLHLNTGTFRHDLARARTFTLAEEIEQLRAA
                     GLALGGSLDNAVVVDRARILNPAGLRSPDEFVRHKLLDAVGDLALAGAPLVGRFVAHR
                     SGHTLNNKLLRALFADRSSWRETSSPALSVAAA"
     gene            4445..>4656
                     /locus_tag="VKQ27_18370"
     CDS             4445..>4656
                     /locus_tag="VKQ27_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90954.1"
                     /translation="MAPPHAISGAMRIRPTLALVASLFICLPLLVSCGSKDENADADK
                     PVAPVEDLYNNGVDALNSQRYDSAND"
BASE COUNT          828 a         1420 c         1580 g          828 t
ORIGIN      
        1 catgctgccg gaaggccacg ccgtcgaggc gatcgatcgg ttgatgcagc tacagcagca
       61 gcacgaactg ctcgaccgac cgcttgccgc gatcgatctg cggctgggtg atcggctcgt
      121 ggtgcggcca cggccggacg agaagaaagc gtcatgacgt ggcacagtgc catcccccac
      181 ctcgcccatc ctctgcgccc ccggcgggga ggagaaaaag tgggagacgc tgatgaataa
      241 catgtcgcgc cccgtgctgc cgccgccgat ggagcccgat gagccggctc gtacgcggaa
      301 tggacgatcg ggtccgtttg gtgtgctgga catcggtacg accaaggttg cctgcctgat
      361 tgggcgcaca gagagcgacg ggacactgcg cgcgctcggg tttggctggc atcgcgggcg
      421 cggggtgcgc ggcgggtcga ttgtcgatct ggatgaagca gaacgcgcga tccgggcctg
      481 cgttggtcag gccgaggaca tggccgatac acagttgcgc gcggtggtcg tgaacctgac
      541 gtgcggacaa cccgaatcgc ggctcttcaa tgtgcaatgg ccggtaggtg ggcgcgcagt
      601 cgaagagcac gacatccggc gcgtagtgaa tgaaggccgc taccgcgcct ctgcggatgg
      661 gcgcgaggtg attcatgcgt tgcccctgac attttcggtt gatcagaccg atcatgtggc
      721 cgatccgcgt gggttgttct gcgaggaact tacggcacgg ctgcacatcg tcgatgcgtc
      781 gtccacagcg ttgcgttcac tcggtgcgtg cattgcgcgc tgcgacttgg atatcggtga
      841 gctggtgtcg gcgccgatgg cggcggggct tgcaacgctg gtcgatgatg aacgcgagct
      901 tggctcaacc gtcattgaca tgggcggtgg caccactggc atggcggtat tcgctgagaa
      961 ccagttgcta cacacggcgc agcttcctgt tgggggcgtt catgtcacca acgatcttgc
     1021 gcggctgctg tcaacgccgg tcgcgcatgc tgagcgactt aagacgctgt acggcaacgt
     1081 gcagaactcg cctgatgacg agcgcgagat gctgccggtg cctctggtgg gcgaggaaga
     1141 gcaccagatc gcgaaggtgc cgcgcagcat ggtggtgaac atcatccgtc caagactcga
     1201 agagacattc gagttggtga aggaacgcct cgacagttcg gggctaacgc gcgcagcggg
     1261 cacgcgcgtg gtgctgaccg gtggcgcgtg ccaactcgcg ggcgcccgcg agctggcagg
     1321 gcgcgtcttg aacaagcagg tgcggcttgg gcggccgatc gcattgcgtg ggctgccgga
     1381 ttcagcatcg ggacccgcgt ttgccacagc ggctgggttg ctcgcatggg ctgcgggtga
     1441 tgggcgcacg atgcacgacg tcgatctgga ggccgaacgg ccgacaggtc ttatccgtcg
     1501 catcgtcaat tttttgaaag accgcgtctg atggccgtcg cgaatcgaaa tatctggcgt
     1561 aactacagac tcaacgactc gacccgacag ggggagatgc tgccatgact ctgaatttga
     1621 cgatcccgca gacagtgcac accgacttca gcccgcggat caccgttatc ggtgtgggcg
     1681 gcggcggtac caacgcggtc gacaacatga tcacggcgaa cctgcagggc gtggagtttg
     1741 ttgtcgccaa cacagacgcg cagcaactca tgcacagccg tgcggatcgg cgcattcagt
     1801 tggggccgca tatcacgcag gggcttggcg ctggcgcaaa gccggagatt ggcaaggccg
     1861 cggccgagga agcgtcggat gaactggcgc ggcatctcga tggtgcacat atggtgttca
     1921 tcaccgctgg catgggcggc ggcaccggca cgggtgcggc acctgtaatt gcgcgcatgg
     1981 cgcgcgagcg gaacatcctg acagttggcg tggtgacgaa gccattcggg ttcgaaggtg
     2041 tccgtcgttc gcgctgcgcc gatcagggca tcgaggagct gcagcggcac gttgatacgc
     2101 tcatcgtcat cccgaatcag aacctcttcc gaatggccaa tgaacgcacc acctggaagg
     2161 aagcgttcaa gatggccgac caggtccttt atatgggtgt gcgtggcgtt accgacctga
     2221 tgatggcgcc gggcctggtg aacctggact tcgccgacat ccgcactgtg atggcggaga
     2281 tgggcaaggc gatgatgggc accggcgagg cggagggcga gaaccgcgct atccgtgcag
     2341 ccgaggcggc gatcagcaac ccgctgctgg aagacacgtc gatgtctggc gcgcaagggc
     2401 tgctgatcaa catcactggg ggcgatgatc tgacgctgtt cgaagtcgat caggcggcca
     2461 accgcatccg cgaggaagtg gacgaggaag ccaacatcat cttcggttcg gcgatcgatg
     2521 agacgctgtc gggccggatc cgcgtgtcgg ttgtagctac gggcatcgac tcgccggcac
     2581 agggccaggg cgagcgtcca cgcctcgtcg ccgttggcgg cagcgcaatg ccgatgccgc
     2641 agccaatccc gctgcccatg cacgcggccg ccgcggctgt taccatgcca gcgggcgcgg
     2701 gactggcgcc cgcgccagtc atgagcgcac aggggcatcc gctgcgccag ccggcacccc
     2761 aactggtcaa cgtggcagac gcagtgccgg acacggcgtc gatgatggaa atggcatcaa
     2821 tgtcagcgcc acagccgatc ccccacccgg tagggccggt ccactccatg acgcctgtcc
     2881 attcagtaac ggcgagcgtg caagcagttg gcatcacgca gccgcgcgcc gcatccggcg
     2941 cgtcgcgtgc tagtctgttc gcagagccgc ggcggccggc cggcgaccct gccgcaccgc
     3001 aggttgcccc ggaatcaacg cgccccagcc tgttcagcac cgtcaccggc gcgttccgcc
     3061 gccgccagca ccccggcgcc gtggaaccgg ccgcgccggt ccgcgcggag cctgtggtgc
     3121 aggcggttgc gcgggcggag ccgccacagg cctcagtgcg acagaccgct ggcgaggaag
     3181 tcggactcga tataccggcc ttcctgcgcc gccagtcgtc gtagatcgcg tgcaacagca
     3241 catcgcgtga ttcgcgggtg gactgaggcg acatcgatgg ggaacggctg agcggcaatc
     3301 gccgtcgtgt ctccgagtcg gtgccacggg ggccattttc agggctggtc cggtcgactc
     3361 gcgtttgctt cgcgtatggt gcgacgggca aaaatcctgt ataatcaaaa gattgggcgg
     3421 aaatatttag caacaatcgt tgactggcca ttaccgcggc gcacaattac tttgcctcaa
     3481 attggatgaa gagacgaggc gcatggacgg tctgccggtt tctctcgcgt cggaagatgg
     3541 ctgtgctgag ctggccactg gcgtccagcg taccctgaag gcagccattt cctgcgttgg
     3601 tgtcggcctg cacagtggca agcgcgtgac cctgacacta cgcccggccg gcgtgaacca
     3661 aggcatcgtg ttccggcgca ccgaccttcg gcgcgatatt ccggcccgct atgaccacgt
     3721 ggtggatacg cggctatgca ctgtgctggg aaacggcgga gcgcgcgtaa gcacggtcga
     3781 acacgtcatg gccgcgctct ctggctgtgg catcgacaac gccatcgtcg aactggatgg
     3841 accggagccg ccggcgctcg atggatccgc ggcgcagttt gtattcctga tcgattgtgc
     3901 aggcataatc gagcaggacg aacctcgttc cgccatcgag gttcggcggg tcgtgcgcgt
     3961 gacggatggc gaggcgtttg ccgagttccg ccctggaatg ttcgggctcg acatggctct
     4021 gtccattgac ttcgcggccg cggcgatcgg ccgccaggct ctttcgctgc atttgaacac
     4081 tggcacgttc cgccacgacc tggcgcgtgc gcgaacattc acgcttgctg aagagatcga
     4141 gcagctccgg gcggccggcc tcgcgctggg cggcagcctc gacaacgccg tcgtcgtcga
     4201 tcgggcacgg atcctgaatc cagccggcct gcgatcaccg gacgaattcg tccgtcacaa
     4261 gttgctggat gcggtgggcg atctggcact tgccggggct cccctggtgg gccgcttcgt
     4321 ggcgcaccgc tccggccaca cgctgaacaa caagctgctt cgcgccctgt tcgcggaccg
     4381 gtcctcatgg cgcgaaacct cgtccccggc actttccgtt gcggctgcct gaccagcact
     4441 tcgcgtggct ccgccgcatg ctataagcgg cgcgatgaga atccgcccaa ctcttgccct
     4501 ggtcgcaagc ttgttcatct gtctgccgct gctcgtgagc tgcggctcca aggatgagaa
     4561 tgcggacgcc gacaagccgg tggcgccggt tgaagacctg tacaacaacg gtgtcgacgc
     4621 gctgaactcg cagcgatacg actccgcgaa cgatca
//
LOCUS       DATGEM010000599         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1121761, whole genome shotgun sequence.
ACCESSION   DATGEM010000599 DATGEM010000000
VERSION     DATGEM010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121761"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1879)
                     /locus_tag="VKQ27_18375"
     CDS             complement(209..1879)
                     /locus_tag="VKQ27_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HLY90955.1"
                     /translation="MLPETAPFPAEQIVALDRIISGSSVEQRTWLSGFLAGYQAATTP
                     QPAAVAPPARRVPLTVLFATESGNAESLADGARKAASKLGFAARVLDMADASPAQIAG
                     AQNLLVIASTWGEGDPPQRAVDFFSALMADDAPRLDGLRYAVLALGDRAYARFCETGR
                     MFDERFAALGATRVAERIECDLDYEAPAGTWIDGTLGILQAETGEPEAGAVIHVDFAR
                     PAADGATWTRARPFEAEITERVRLSGSRSTSDTWHVELSLAGSGIEYEPGDSLGIVPS
                     NDPALVDAVLHAAGLAGNAQLRARLQHQHDITTLTHGPRREGWQVIDMLKAEHRQWSE
                     DELLSLLRPLAPRYYSIGSSGKAVPDEAHLLVAAVRYEAHGRTRNGVASVDIAERRKA
                     GEQLRVFLKPNQHFRLPGDTDRPIIMIGPGTGVAPFRAFMQERDAVGARGRNWLVFGH
                     RNYTHDFLYQLEWQDLLKRGVLTRLDVAFSRDQPEKRYAQHVLWEARRDLHAWVQDGA
                     AIYVCGDANAMAKDVHITLQRILGEHGTDGVAALDALRREGRYLRDVY"
     gene            complement(1869..2651)
                     /gene="cobA"
                     /locus_tag="VKQ27_18380"
     CDS             complement(1869..2651)
                     /gene="cobA"
                     /locus_tag="VKQ27_18380"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HLY90956.1"
                     /translation="MIVEPGTVWLVGAGPGDPGLLTVKAAQVLAQANVVLYDALVSPE
                     ILALAPQARLEPVGKRAGRDRTPQMRINQRLIDLARANLRVVRLKGGDPLVFGRGGEE
                     ALALAAAGISCQIVPGISAGIGAAAAAGIPVTHRGMAHSVAFVTGHDSSGGVPDLNWA
                     ALARGADVLVLYMALRQIGAIAAHLREAGRAASEPVAFITEATTPRQSVTSTTLADAE
                     RIAATIPRQAPTLIVVGPVVALRPLLAGQAIQSPLEKERHAA"
     gene            complement(2648..3193)
                     /locus_tag="VKQ27_18385"
     CDS             complement(2648..3193)
                     /locus_tag="VKQ27_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY90957.1"
                     /translation="MIAITLDPAVAPIAVVGRGPAALRRFVKLRAGGTHQPLLFSDQP
                     DSMAAPADALRTRLPGTDDLDGLRALWIAGLPDDEAAALAAAARARRVLVNVEDRPAL
                     CDFHNVAEVRRGDLLLAVSTNGGSPRLAARIRARLATEYGANWAERLEMIRARRAVWQ
                     RQGGDLSALTDALLEANGWLA"
     gene            3523..3987
                     /gene="rplM"
                     /locus_tag="VKQ27_18390"
     CDS             3523..3987
                     /gene="rplM"
                     /locus_tag="VKQ27_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLY90958.1"
                     /translation="MKTTLSLKPAEVRKGWVLIDAEGVVLGRLAAIIANRLRGKHKPQ
                     FTPHVDCGDNVVVVNAGKVRVTGNKPDQSVFYYHTGYPGGIKGRSVRQRLESQHPERV
                     LEKAVERMITRGPLQRKQMKHLYVYAGPTHKHEAQQPVTLDVAALNPKNKRG"
     gene            3995..4522
                     /gene="rpsI"
                     /locus_tag="VKQ27_18395"
     CDS             3995..4522
                     /gene="rpsI"
                     /locus_tag="VKQ27_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLY90959.1"
                     /translation="MSGTTRTLADLKDALAAQQVAQATTGTVTGGPAVPSDQPAREPK
                     RDAFGRSYATGRRKNAVARVWIRPGKGEITVNDKQVVQYFARPVLRMLITQPFLVADR
                     YNQFDVYCTVTGGGLSGQAGAVRHGISRALTYYEPDLRGILKVAGFLTRDPRVVERKK
                     YGKAKARRSFQFSKR"
     gene            4547..>5439
                     /gene="argC"
                     /locus_tag="VKQ27_18400"
     CDS             4547..>5439
                     /gene="argC"
                     /locus_tag="VKQ27_18400"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLY90960.1"
                     /translation="MATKPSVFIDGEAGTTGLGIRDRLRALPDVELKSLPSDKRRDPA
                     ARRNVMAEVDLVILCLPDDAARESVAMADSLGNAAPKVLDASTAHRVHPDWAYGFPEM
                     QEGQAAKITAARKVANPGCYPTGAIGLIRPLVDAGIIPGDYPITINAVSGYSGGGRSM
                     IEDHEQHGGPAFELYALKLEHKHLPETQLYSGLSRRPIFVPSVGHYRQGMLVSVPLHL
                     DALNGRPDGADLQAALDARYRGCDFVRVVAPSADGKLEPEALNDTNQLELSVYANDKQ
                     RQAVLVARLDNLGKGASGAAV"
BASE COUNT          972 a         1695 c         1859 g          913 t
ORIGIN      
        1 gaagatgaag gccttctcca tcttctggcg gcctcgctcg gagcggaggt cgcgatcgtc
       61 ctgcaggtag ataccgtgga acttggtcag ttgctgatcg ttgtcggcca gcgaaccggt
      121 ttcggcacga gtcaagccgt ccgcgatcgt gccgcggaga aagcgacttt ccgatttgat
      181 ccgttcgtta gcggaggggg gacgaacttc agtagacatc gcgcaggtac cttccttcgc
      241 ggcgcagcgc atcgagcgcg gcgacaccgt ccgtgccgtg ctcgccaaga atgcgttgca
      301 aggtgatgtg cacatctttg gccatcgcgt tggcatcgcc gcacacatag atagcggcgc
      361 cgtcctggac ccaggcatga aggtcgcgcc gggcctccca cagcacgtgc tgcgcgtagc
      421 gcttttccgg ctgatcacga gaaaatgcca catccagccg cgtcagtacg ccgcgcttca
      481 gcagatcctg ccattcgagc tgatacagga agtcgtgcgt gtagttgcga tggccgaaca
      541 ccagccagtt gcggccacgc gcgcccacgg catcgcgctc ctgcatgaag gcgcggaatg
      601 gcgcgacgcc ggtgcctggg ccgatcatga tgattggccg atcggtgtca cccggcaggc
      661 ggaagtgttg gttcggctta aggaacacgc gcaattgctc gcctgccttg cggcgctcgg
      721 cgatatcgac ggaagcgaca ccgttgcggg tgcggccatg tgcctcgtag cgcaccgcgg
      781 cgacaagcag atgcgcttcg tccggcaccg ccttgccgct ggagccgatc gagtaatagc
      841 gcggcgccaa tggccggagc agggacagca gttcgtcctc ggaccactgg cgatgctcgg
      901 ctttcagcat atcgatcacc tgccagccct cgcggcgtgg cccgtgggtc agcgtggtga
      961 tgtcgtgctg gtgttggagc ctcgcgcgca actgcgcgtt ccctgccagg ccagcggcgt
     1021 ggagcaccgc atcaaccaga gccggatcat tgctgggcac gataccgagc gaatcgcctg
     1081 gttcgtactc gataccggat ccggccagcg acaactccac atgccaggta tcagacgtcg
     1141 aacggctgcc ggagagacgc acgcgttcgg tgatctccgc ctcgaagggg cgtgcccgtg
     1201 tccaggtggc gccatcagcg gccgggcgcg cgaaatcgac gtggatgaca gcgccggctt
     1261 cgggctcgcc ggtctctgcc tgcaatatgc ccaacgtgcc gtcgatccag gttccggccg
     1321 gtgcctcata atccaggtcg cattcgatcc gctcggccac ccgcgtcgcc ccgagcgccg
     1381 cgaagcgctc atcgaacatc cgtcccgtct cgcagaaccg cgcgtaggcg cgatcaccca
     1441 gtgccagcac cgcgtagcgc agtccatcca ggcgcggtgc gtcatctgcc atcagggccg
     1501 agaagaagtc gaccgcgcgc tggggcggat cgccttcgcc ccaggtgctg gcgatcacca
     1561 gcaggttctg tgcgccggcg atctgggctg gcgaggcatc cgccatgtcc agcacgcgtg
     1621 cggcgaagcc gagcttgctg gcggccttgc gcgcgccgtc ggccagcgac tcggcgttgc
     1681 ctgactcagt ggcgaacagc acggtcagcg ggacacggcg tgcaggcggc gcgaccgcgg
     1741 cgggctgcgg cgtggtcgcc gcctggtaac ccgcgaggaa accgctcagc caggttcgtt
     1801 gctccacgct gctgcctgag atgatgcggt ccagcgcgac gatctgttcg gcggggaagg
     1861 gggctgtttc aggcagcatg acgttctttc tccagcggcg actgaatggc ttggcccgct
     1921 agcaacggcc gcaacgcgac gaccggcccg acaacaatga gtgttggagc ctggcgcggg
     1981 atggtcgcgg cgatgcgctc ggcgtcggcc agcgtggtgc tggtgacact ttggcgaggt
     2041 gtggtggctt cggtgatgaa ggcgaccggc tccgatgcag cgcggcccgc ttcccgcaga
     2101 tgggcggcga tcgcaccgat ctgccgcagt gccatgtaca ggaccagcac gtcagcgccg
     2161 cgcgccagag ccgcccagtt cagatcaggt actccgcccg aggaatcgtg gcccgtgacg
     2221 aaggcgacgc tgtgcgccat tccgcgatgc gtgaccggga tgccggcggc agcggcagcg
     2281 ccaattcccg cactgatacc aggcacgatc tggcaagaga taccggctgc ggccagggca
     2341 agggcctcct cgccaccccg gccgaacacc aggggatcgc cgcctttcag acgcaccacc
     2401 cgaaggttgg cccgtgccaa gtcgatgagg cgctggttga tgcgcatctg cggggtgcgg
     2461 tcgcggcctg cgcgcttgcc gacaggttcc aggcgggctt ggggggcgag agcgaggatt
     2521 tccggcgaga ccagcgcgtc gtacaggaca acgttggcct gcgccaatac ctgcgcggct
     2581 ttcaccgtca gcaggcctgg atcgccggga cccgcgccca ccagccagac ggtgccaggc
     2641 tcaacgatca tgccaaccac ccgttcgcct cgagcaaggc atcagtcagc gctgacagat
     2701 cgccgccttg gcgctgccag acagcccgcc tggcgcgaat catttcaagg cgctcagccc
     2761 agttggctcc gtattcggtg gccagccgtg cgcggatgcg cgccgccagg cgcgggctgc
     2821 cgccattggt ggagaccgcg agcaacaggt cgccacggcg gacctccgcc acgttatgga
     2881 agtcgcacag ggcggggcgg tcctcgacgt tgaccagcac acgcctcgcg cgcgcggctg
     2941 ccgccagcgc ggcagcctcg tcatcaggca gaccggcgat ccagagggca cgcaggccgt
     3001 cgagatcgtc tgtcccggga agacgggtcc ggagggcgtc ggcgggcgcc gccatgctgt
     3061 ccggctggtc cgaaaacagc aggggttgat gcgtgccccc tgcacgcaac ttaacgaaac
     3121 gccggagggc agccggtcca cggccgacga cggcaatcgg cgcaaccgcg ggatcgagag
     3181 tgatggcgat catcgcggtg aaccgctcgg gcggaatgtg ttgtctcggg ccatcggaat
     3241 ccctcggcgt acggtcgatg cggattgcta agcatgtggc gaacctgcgg ccaaaagaga
     3301 agaatttcct tcgcaaggca agatgcatgg ctaaaataac aaaattcatc tacaacgagg
     3361 ccagacaggg aatggcgctc ctgccgttgg cggcgtgtgc gaacagtctg tcctgtgccg
     3421 tggatgggtg tgtggtagga aattaccaca tcatgcaaag cttgacctgg caggcctttc
     3481 cggccatatg ccgccgccaa atcgcaggat gccacgccaa tgctgaagac caccctctcg
     3541 ctgaaaccgg cggaggtgag gaagggctgg gtgctgatcg acgccgaggg cgtcgtgctc
     3601 ggccgcctcg ccgcgatcat cgccaaccgg ctgcggggca aacacaagcc gcagttcacg
     3661 ccgcatgtcg attgcggcga caacgtcgtt gtggtgaacg ccgggaaggt gcgcgtgacg
     3721 gggaacaagc cggatcagtc cgtcttttac taccacacgg gctatcccgg cggcatcaag
     3781 ggccgcagcg tccgccaacg tctggaaagc cagcatccgg agcgcgtgct tgagaaggcc
     3841 gtggagcgta tgatcacccg cgggccgttg cagcggaagc agatgaagca cctgtatgtc
     3901 tatgccgggc cgacccacaa gcacgaggcg cagcagcccg tgactctcga cgtcgccgca
     3961 ctgaacccga agaacaagcg aggctgatcc cgcaatgtct ggaactactc gtaccctcgc
     4021 cgatctcaag gacgcgctcg ccgcgcaaca ggtggctcag gccacaacgg gaactgtcac
     4081 cggcggaccc gcggttccct ccgatcagcc ggcgcgtgag ccgaagcgcg atgcattcgg
     4141 gcgctcctat gcgaccgggc ggcgcaagaa cgcggtagcg cgcgtgtgga tcaggcctgg
     4201 caagggtgag atcactgtca acgacaagca ggtcgtgcag tacttcgcgc ggcctgtgct
     4261 gcgcatgctg atcacgcagc ctttcctggt ggcggatcgc tacaaccagt tcgatgtgta
     4321 ttgcaccgtg accggcggcg gtttgtccgg ccaggcgggc gcggtgcggc acgggatttc
     4381 ccgggcgctc acgtattatg agccagacct gcgaggtatt ctgaaggtgg ccgggttcct
     4441 gacacgcgat cctcgcgtgg tggaacgcaa gaagtacggc aaggcgaagg cgcgacgaag
     4501 cttccagttc agcaagcggt aagtttgtca tggatggagg tgtgttgtgg cgaccaagcc
     4561 aagtgtgttc atcgatggag aagcgggaac aaccggtcta ggcattcggg accggctgcg
     4621 cgcactgccg gacgttgagc tgaagagcct gccgtccgac aagcggcgcg atccagctgc
     4681 gcggcgcaat gtcatggccg aggtggatct cgtgatcctc tgcctgcccg atgacgcggc
     4741 acgcgaatct gtggcgatgg ccgacagtct gggcaatgcg gcgccgaagg tgctggatgc
     4801 aagtaccgcg catcgggtgc atccggactg ggcgtatggc tttcctgaaa tgcaggaagg
     4861 gcaggcggcg aagatcactg ccgcgcgcaa ggtcgccaat cccggatgct atccgactgg
     4921 ggcgatcggt ttgatccggc cgctggtgga tgccgggatc attccgggcg attatccgat
     4981 caccatcaat gcggtcagtg gctattccgg cggcggtcgg tcgatgatcg aggatcacga
     5041 gcagcatggt ggtcccgcgt tcgaactgta tgcgctcaag ctcgagcaca agcatttgcc
     5101 ggagacgcag ctttattccg ggctgtcgcg gcgtccgata ttcgtgccgt cagtcgggca
     5161 ttatcggcaa gggatgctgg tgtcggtgcc gctgcatctc gatgcgctga acggcaggcc
     5221 cgatggggct gatctgcagg cggcgctgga tgcaaggtat cgcggctgcg atttcgtgcg
     5281 cgtggtggcg ccgtctgccg atggcaagct ggaaccagag gcgctgaacg ataccaacca
     5341 actggaactg tcggtgtatg cgaacgacaa gcagcgccag gcggtgctgg tggcgcggct
     5401 cgacaatctc ggcaagggtg cgtccggggc ggcggtgca
//
LOCUS       DATGEM010000600         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_258355, whole genome shotgun sequence.
ACCESSION   DATGEM010000600 DATGEM010000000
VERSION     DATGEM010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258355"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VKQ27_18405"
     CDS             complement(<1..929)
                     /locus_tag="VKQ27_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY90961.1"
                     /translation="MSEDFYRIRRLPPYVFAEVNAMKAAARAAGEDIVDLGMGNPDGA
                     PAPHVIEKLAEVARDPHAHGYSASKGIKGLRKAQASYYARRFNVELDPENEVVVTLGS
                     KEGLANLAQAITAPGDVVLAPNPSYPIHTFGFIIAGASIRSIPAAPGPDFFRRLEHAV
                     AYTVPKPKVLVIGYPSNPTAYVADLDFYTKVVAFAKEHGLWVLSDLAYAEIYFGDTPT
                     PSILQVPGAKDVAIEFTSMSKTYSMAGWRIGFAVGNRQLIAALTRVKSYLDYGAFTPI
                     QAAAVAALNGPQDIVEQNRQLYKRRRDVLVESFG"
     gene            1050..2807
                     /gene="phaC"
                     /locus_tag="VKQ27_18410"
     CDS             1050..2807
                     /gene="phaC"
                     /locus_tag="VKQ27_18410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HLY90962.1"
                     /translation="MTGALDAIQPTLDELQHWTGVMGRAQQMILDYAARQSLRPAEQP
                     PLPDLAKLLSPPMHFGIDTQALMDAQADFWKDSLTLWQRFLNPAAATPSDAAEDRRFT
                     APQWRDHPLFDMLRQSYQLVSSHMLRSVETIGGLSAKQREQLRFAMRSLTEAMAPSNF
                     TLTNPQALEKAITTRGESILKGLENLLGDLARGQLTQSSPSAFELGRNIAVTPGKVIH
                     ETPLFQLIHYAPVTESVAKIPLVIFPPWINRFYILDLNPKKSFVHWALEQGLSVFMVS
                     WKSADESIAGVTLDDYVAAQIEAIDVVRDLLGVEATHAIGYCVAGTTMAATMALLAAR
                     GEGGKVATATFFTTQIDFSAAGDLSLFVDDTQLDLVAGLSGDKGYLDGRYMAATFNLL
                     RGRDLIWNYVVQNYLMGEDYPAFDLLHWNGDITNLPAAWHQAYLRDFYRDNLLVRPGG
                     IHVAGVPIDLHRVTTPTYIQAGRDDHIAPPESVWKITEHFGGELRFVLAGSGHIAGVV
                     NPPSAGKYQYWTCDEPAASLETFKTLARETKGSWWPDWISWIQAHDGETVPATRARLP
                     GKGKRKAIEDAPGRYVAQR"
     gene            2945..3919
                     /gene="phaP"
                     /locus_tag="VKQ27_18415"
     CDS             2945..3919
                     /gene="phaP"
                     /locus_tag="VKQ27_18415"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HLY90963.1"
                     /translation="MAENTNKPTPNAPSKKEPPAKPAVAAKAAPVEIKPVNVPKPAPI
                     EAKPAPVEPKPVTAIKPTPVDVKPVTAAKPVVTSKEPSAVAAPKAAPVAAKPAPEAPK
                     TKSAPPAPAPAPKPAEVVASKRDEPTVGVTTTPPIASPVPSVASVAKHEAPPITAAAI
                     TEGSRIMNETMTKAQETAKKFTADAGERVQALFGEVSERAKGAYEKSVKLTEELTEFQ
                     KGNVEAFVESSKLAAKAAESLGQEAADYARKSFENSTAAMKTFASAKSPSELFKLQSD
                     YMKSSFDAAVAESSKVTEAWLKLAGDVAQPLSNRLAVAVEKVKTTTTM"
     gene            4157..4483
                     /gene="clpS"
                     /locus_tag="VKQ27_18420"
     CDS             4157..4483
                     /gene="clpS"
                     /locus_tag="VKQ27_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLY90964.1"
                     /translation="MAEGKDGDDAGTGLGVATRTRTRTKKPSLYKVLMLNDDYTPMEF
                     VVLCLQRFFRMNIEDATRVMLHVHQKGVGVCGVFSHEVAETKATQVVDYARQHQHPLQ
                     CTIEKA"
     gene            complement(4500..>4776)
                     /locus_tag="VKQ27_18425"
     CDS             complement(4500..>4776)
                     /locus_tag="VKQ27_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90965.1"
                     /translation="VETAHTFWATATEVLERCRAGEGRAIFPTRRLLERLARFPRFVD
                     ARAEAERLPQRIITPWVEQRADGAWLRIPADAGYPVTEERVETAMRY"
BASE COUNT          888 a         1638 c         1496 g          754 t
ORIGIN      
        1 ccaaagctct cgaccagcac gtcgcggcgg cgcttgtaga gctggcgatt ctgctcgacg
       61 atgtcctgcg gtccgttgag cgcggccacc gccgccgcct gaatcggcgt gaaggcgcca
      121 taatccagat aggacttcac ccgcgtcagc gcggcgatca actggcggtt gccgaccgca
      181 aagccaatcc gccagcccgc catcgaatag gtcttggaca tcgaggtgaa ctcgatcgcg
      241 acgtccttgg cgcccggcac ctgcaggatc gagggggtcg gcgtgtcgcc gaaatagatc
      301 tccgcatagg cgaggtcgga cagcacccac agcccatgct ccttcgcgaa ggcgaccacc
      361 ttggtgtaga aatcgagatc tgcgacatag gccgtcggat tggacggata gccgatcacc
      421 agcaccttgg gcttgggcac ggtgtaggcg acggcatgct ccagccggcg gaagaagtcc
      481 ggccccggcg ccgccggaat cgatcggatc gaggcgcctg cgatgatgaa gccgaaggtg
      541 tggatcgggt agctggggtt gggtgccagc accacgtcgc cgggcgccgt gatcgcctgc
      601 gccagattgg cgaggccctc ctttgagccg agcgtgacga ccacctcatt ctctggatcg
      661 agctcgacgt tgaagcggcg ggcataataa ctggcctgcg ccttgcgcag ccccttgatg
      721 cccttggacg cggaatagcc gtgcgcgtgc ggatcgcggg ccacttcggc cagcttctcg
      781 atcacatgcg gggccggcgc cccatccgga ttgcccatgc cgagatcaac gatgtcctcg
      841 cccgccgcgc gcgctgccgc cttcatcgcg ttcacttcgg cgaacacgta ggggggcaga
      901 cgacgaatgc ggtagaaatc ctcggacatg ttcgaaagac ctttcggatg gctcgccatt
      961 gtcatggctc ccgcgttata gcgtcagaga atgcgccgtt acaggcgcgt ttcgacggga
     1021 gaggtcgatt ggcggattcg gatcgcacga tgacgggcgc gctggacgcg atccagccca
     1081 cgctggacga gctccagcac tggaccgggg tgatgggccg tgcccagcag atgatcctcg
     1141 actatgccgc acgccagagc ctccgcccgg ctgagcagcc tcctctcccc gatctcgcaa
     1201 agctgctctc gccaccgatg cactttggca tcgacacaca ggccctcatg gatgcgcagg
     1261 ccgatttctg gaaggatagc ctgacgctct ggcagcgttt cctcaatccc gccgctgcca
     1321 cgccgtccga tgcggccgag gatcgacgct tcaccgcccc ccaatggcgc gaccacccgc
     1381 tgttcgacat gctgcgccaa tcctatcagc tcgtctctag ccacatgctc cgctcggtcg
     1441 agacgatcgg ggggctttcg gccaagcagc gcgagcagct gcgcttcgcg atgcgatccc
     1501 tcaccgaggc gatggcgccg agcaacttca ccctcaccaa cccgcaggcg cttgaaaaag
     1561 ccatcaccac caggggcgag agcatactca aggggttgga gaatctgctc ggcgatctcg
     1621 cccgcggcca gctcacccag tcgagcccga gcgcgttcga actgggccgc aacatcgcgg
     1681 tgacgcccgg caaggtgatc cacgaaacgc cgctgttcca gctgatccac tatgcccccg
     1741 tgaccgagag cgtcgcgaag atcccgctcg tgatcttccc gccctggatc aaccgcttct
     1801 acatcctcga tctcaacccc aagaagagct tcgtccactg ggcgctggag caggggctga
     1861 gcgttttcat ggtctcgtgg aagtcggccg acgagagcat cgccggcgtc acgctcgacg
     1921 actatgtcgc ggcccagatc gaggcgatcg acgtggtacg cgatctgctc ggcgtcgagg
     1981 ccacgcatgc gatcggctat tgcgtcgctg gcaccacgat ggcggccacg atggcgctgc
     2041 tcgcggcaag gggcgagggt ggcaaggtcg ccaccgcgac cttcttcacc acccagattg
     2101 atttcagtgc cgcaggcgac ctgtcgctgt tcgtggacga cacgcaactc gatctcgtcg
     2161 ccggactttc gggcgacaag ggctatctcg acggccgcta catggccgcc accttcaacc
     2221 tgctgcgcgg gcgcgatctg atctggaact atgtcgtcca gaattatctg atgggcgagg
     2281 attatccggc gttcgacctg ctccactgga acggcgacat caccaacctg cccgccgcct
     2341 ggcaccaggc ctatctgcgc gacttctatc gtgacaacct gctcgtccgg cccggtggga
     2401 tccacgtcgc cggcgtgccg atcgatctcc accgcgtaac cacccccacc tatatccagg
     2461 caggccgtga cgatcatatc gcgccgcccg agagcgtgtg gaagatcacc gagcatttcg
     2521 gcggcgaact gcgcttcgtg ctcgccggat cgggccatat cgccggcgtc gtcaacccgc
     2581 cgagcgccgg caaatatcaa tattggacct gcgatgagcc ggccgcctcg ctcgaaacgt
     2641 tcaagactct ggcgcgcgag accaagggca gctggtggcc cgactggatc agctggattc
     2701 aggcgcatga cggcgaaacg gtgcccgcga cgcgcgcgcg ccttcccggc aagggcaaac
     2761 gcaaggccat cgaggacgcg cccggacgct acgtcgccca gcgataatac acgtttcgtc
     2821 gcagcagcga tgcgggtcgg cgcaggctta tgctgcgctg cacaatacct cttgcaccgt
     2881 cgccgcgaat cggatattgt gcagcgcaac atgagtgtaa tggttgcagc cttgggagtg
     2941 gggcatggcc gagaacacga ataagccgac gccgaacgca ccttcgaaaa aggagccgcc
     3001 ggccaagccg gctgtcgcgg cgaaagccgc accggtcgag atcaagccgg tcaatgttcc
     3061 gaagcccgcc ccgatcgagg ccaagccggc gcctgtcgaa cccaaaccgg tcacggccat
     3121 caagccgaca cctgtcgacg tcaagccggt cacggccgcg aagcccgttg tgacatccaa
     3181 ggaaccatct gccgttgcgg cccccaaggc cgcaccggtg gctgccaagc cggcgcccga
     3241 agcgcccaag accaagtcgg cccccccggc cccggcgcct gcgccgaagc cggcagaagt
     3301 ggtcgcatcc aagcgggacg aaccgacggt cggggtcacc acgaccccgc ccatcgcatc
     3361 ccccgtcccg tccgtagcgt ctgtggcgaa gcacgaggca cccccgataa cggcggctgc
     3421 catcaccgag ggaagcagga ttatgaacga gaccatgacg aaggctcagg aaaccgccaa
     3481 gaagttcacc gccgacgccg gcgagcgcgt ccaggcgctg ttcggcgagg tcagcgagcg
     3541 ggccaagggt gcctacgaga agagcgtcaa gctgaccgag gagctcaccg agttccagaa
     3601 gggcaatgtc gaggcgttcg tcgaatcctc gaagctggct gccaaggccg ccgagtcgct
     3661 cggccaggaa gccgccgact acgcccgcaa gtcgttcgag aacagcaccg ccgcgatgaa
     3721 gactttcgcg tcggccaagt cgccgagcga actcttcaag ctccagagcg actatatgaa
     3781 gtcgtcgttc gacgccgccg tggcggagag ctcgaaggtc accgaggcgt ggctcaagct
     3841 ggctggcgac gtcgcccagc cgctgtcgaa ccgcctcgcg gtcgccgtcg agaaggtgaa
     3901 gaccacgacc acgatgtgat ccgctccggc tgatccgaga cgaacggccg cccctccccg
     3961 aggggcggcc gctttgattc ggggccagcc gtaccacgct ggttcgcccg gtcgttgccc
     4021 ggccacgacc attcccgcga tccgcaggcc ccggggccgc cacgcctctt gccctccggc
     4081 cgcgcgatgc catattctgc ggacagccat gcatatccac atcgacagca tcgacacggc
     4141 gagcgcggtc cgcgccatgg ccgaagggaa agacggggac gacgccggca ccggcctggg
     4201 cgtcgcgacc cggacccgca cgcgcaccaa gaagccttcg ctctacaagg tgctgatgct
     4261 gaacgacgat tatactccga tggagttcgt cgtgctgtgc ctgcagcgct tcttccggat
     4321 gaacatcgag gacgcgacgc gcgtgatgct ccacgtccac cagaagggtg tgggcgtgtg
     4381 cggcgtgttc agccacgagg tggccgagac caaggcaacg caggtggtcg actatgcgcg
     4441 ccagcaccag catccgctgc agtgcacgat cgaaaaggcc tgatcggccg tccggcctct
     4501 caataacgca tcgccgtctc gacacgctct tcggtcaccg gataacccgc gtccgccgga
     4561 atgcgtagcc acgccccgtc ggcccgctgc tcgacccagg gcgtgatgat ccgctgcggc
     4621 aggcgctcgg cctcggcgcg ggcatcgacg aagcgcggga agcgggccag ccgctccagc
     4681 aggcgccgcg tcgggaagat cgcccgcccc tctcccgctc ggcagcgttc cagcacctcc
     4741 gtcgcggtcg cccagaaggt gtgcgcggtc tccact
//
LOCUS       DATGEM010000601         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_382902, whole genome shotgun sequence.
ACCESSION   DATGEM010000601 DATGEM010000000
VERSION     DATGEM010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382902"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VKQ27_18430"
     CDS             complement(<1..762)
                     /locus_tag="VKQ27_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLY90966.1"
                     /translation="MSVAHLSLRRRLHERGLDAVTLLVLPGVLFVLALFIYPFIYGLI
                     LSFQPKDGGGWLTNYQKFFSEPFLYETIWLTLQIAIPATLLNTVLSVPVALRVRLMKR
                     QKLLTTILVIPITLGTVMVADGLLTYLGPRGWLNRVLLDFGITDSPVRLIHNYWGVML
                     SLVISGFPFTFLLTLSYVTGIDPALEQAGAMLGAKARRRFFRILLPLLAPGLAITLAL
                     AFVQAFTVFPSAILLGAPAGPTRVISIIAYQAAFEQ"
     gene            complement(759..1817)
                     /locus_tag="VKQ27_18435"
     CDS             complement(759..1817)
                     /locus_tag="VKQ27_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY90967.1"
                     /translation="MPIASRDFRELRLEGVGRDFGQLHALRDVNLTVRRGEFVALLGP
                     SGCGKSTALNCISGLLQPTDGGIWLDDKRLDDIGPEDRGFGMVFQNYALFPHMTVRRN
                     IGFGLTTQRRPRDEIERRVNEALALVRLETQAEKLPGQLSGGQQQRVAIARAIVVEPP
                     LVLMDEPLSNLDAKLRLEMRAEIRRIHTMLGCSTIYVTHDQEEALSLADRIMVMIDGQ
                     PRQIGTPEDLYVRPTSADVAEFMGYRNLLTSPARTVPSGSGVTVMIGGAHVLGTAMAP
                     VDGHATVAIRPDDLHPTVDGPIAATVEIAEYHGRDFYAVARGKEGEDLYFRSSQRISP
                     GEIVHLSALPERVLVFPA"
     gene            complement(1884..3074)
                     /locus_tag="VKQ27_18440"
     CDS             complement(1884..3074)
                     /locus_tag="VKQ27_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY90968.1"
                     /translation="MTTPKVDRRTLLGGMAGLAALGAGRAFAASAPALPSSPVTLNVV
                     DVAGQLQLTQGAIDAFAKANANLVSKITYSQAPAPELPGKIKAQQAAKSVDIDLVLTG
                     TDALSAGIDQGLWIPVVTDYASVLPKLQDIYQPGAWKMQGLAQNQAVCVVFCPAGPIL
                     EYMPDAVKTPPKTADDLLAWTKAHPKRFIYARPANSGPGRTFLQGLPYILKDKDPMDP
                     KDGWDKTWAYLVELGKNIEYYPTGTGAVMKELGEGSRDMIASHLGWDLNPRILGVVPK
                     EAEIGTLDGFHWVTDAQYWAIPKGVSNDKLAVLLKMTDYMLSKPAQAMTYDKGYFYPG
                     PAVKDVPLSMAPQASQDAVGSVRRPFIDKLIETVPSEVPLPADKLVYAFQRWDQQVGA
                     KVGK"
BASE COUNT          700 a         1161 c         1027 g          632 t
ORIGIN      
        1 ctgctcgaac gccgcctgat acgcgatgat cgagatcacc cgcgtcggtc ccgccggcgc
       61 gcccaacagg atcgccgagg gaaacacggt aaacgcctgc acgaacgcca gcgccagggt
      121 gatcgcaagc cccggtgcca acagcggcaa caggatgcgg aagaaccgcc gccgcgcctt
      181 ggcgcccagc atcgcgccgg cctgctcaag cgcggggtca atgccggtga catacgacag
      241 cgtcagcagg aacgtgaacg gaaagccgct gatcaccagc gacagcatca cgccccaata
      301 gttgtggatc agccgtaccg gcgaatcagt gatgccgaaa tccaacagga ccctgttcaa
      361 ccagccacgc ggaccgagat aggtcagcag accgtcggcc accatcacag tgccaagcgt
      421 gatcgggatc accagtatcg tcgtcagcag cttctgccgc ttcatcagtc gcacgcgcag
      481 cgccactggc acagacaaca ccgtgttcag caaagttgcc gggatggcaa tctgcaaggt
      541 gagccagatc gtttcataca ggaatggctc gctgaagaat ttctggtagt tcgtcagcca
      601 tcctccgcca tccttcggtt ggaacgacag gataagcccg tagatgaacg gatagatgaa
      661 cagcgctagc acgaagagca caccgggcag aaccagcagt gtcaccgcat ccaggccgcg
      721 ctcatgcagg cgccggcgca gcgacaggtg cgccacgctc atgcgggaaa caccagcacg
      781 cgctcaggca gcgcggacag atgtacgatt tcaccggggc tgatacgctg gctggagcgg
      841 aagtacaggt cctcaccttc cttgccacgc gccaccgcgt agaagtcgcg gccatgatat
      901 tcggcgatct ccactgtcgc agcgatcggc ccatcaaccg tcggatgcag atcgtccggg
      961 cggatcgcca cagtggcgtg tccatccact ggcgccatcg ctgtgccaag cacatgcgcg
     1021 ccgccgatca tcacggtcac cccgctcccg ctgggcacag tccgtgccgg cgatgtcagc
     1081 agattgcggt agcccatgaa ctcggcgacg tcggcgctgg tggggcgcac gtacagatcc
     1141 tcgggtgtgc cgatctggcg aggctgccca tcgatcatca ccatgatccg gtcggccagc
     1201 gacagcgctt cttcctgatc gtgcgtcaca tagatcgtgg agcagcccag catcgtgtgg
     1261 atacgccgta tctccgcccg catctccagc cgtagcttcg catccaggtt ggacagcggc
     1321 tcatccatca acaccagcgg cggttccacc acgatcgcac gcgcgatcgc cacacgctgc
     1381 tgctgtccac cggaaagctg ccctggcagc ttttccgcct gcgtctccag tcgaaccagg
     1441 gctagcgcct cattgacccg acgctcgatc tcatcgcgag gccgccgctg cgtggtcagc
     1501 ccgaacccga tgttccgccg cacggtcatg tgcggaaaca gcgcgtagtt ctggaacacc
     1561 atgccgaagc cgcgatcctc cggtccgatg tcgtccagcc gcttatcatc gagccagata
     1621 ccgccgtcgg tcggttgcag caggccggag atgcagttca atgctgtgga tttgccgcaa
     1681 ccggatgggc caagcagggc gacgaactcg ccccgcctca ccgtcaggtt gacgtcacgc
     1741 agcgcatgca gctggccaaa gtcacggccc accccttcga gccgcaactc gcgaaagtca
     1801 cgcgatgcga taggcatgtt ggctacccgc tcctcccggt cagagcgggt gaaactcgtt
     1861 catgatgctg gatgagtcca ccgtcatttg ccaacctttg cgccgacctg ctggtcccag
     1921 cgctgaaagg cgtaaaccag cttgtccgcc ggcaacggca cttccgaagg caccgtctcg
     1981 atcaacttgt cgatgaacgg ccgccgcacg ctgccgacag cgtcctggct ggcctgcggc
     2041 gccatcgaca gcggcacatc cttcaccgcc ggaccgggat agaaatagcc cttgtcgtag
     2101 gtcatcgcct gcgccggttt gctcagcatg tagtccgtca tcttcagcag cacagccagc
     2161 ttgtcgttcg atacgccctt ggggatcgcc caatactgcg cgtcggtaac ccaatggaag
     2221 ccgtccaacg tgccgatctc ggcttccttc ggcaccacgc ccaggatacg cggattcaaa
     2281 tcccagccga gatgcgaggc gatcatgtcg cgcgacccct cgccgagttc cttcatcacc
     2341 gcgccggttc cggtcgggta gtattcaatg ttcttgccga gctcgaccag ataggcccag
     2401 gtcttgtccc agccatcttt cgggtccatc ggatccttgt ccttcaggat gtatggcagg
     2461 ccctgaagga aggtgcggcc cggaccagaa ttggcaggac gcgcgtagat gaagcgcttg
     2521 gggtgcgcct tggtccaggc aagcagatcg tccgcggttt tgggcggcgt cttcaccgcg
     2581 tccggcatgt attccagaat cggaccggct gggcagaaca caacacagac cgcctggttc
     2641 tgcgccaggc cctgcatctt ccaggcaccc ggctgataaa tgtcctgcag cttcggcaac
     2701 accgaggcat agtcggtgac cacgggaatc caaaggccct ggtcgatgcc agccgacaag
     2761 gcgtcggtgc ctgtcagcac cagatcgata tcgacactct tggcagcctg ctgggccttg
     2821 atcttacccg gcagctcagg cgccggcgcc tgagaatagg tgatcttgga gaccaaattc
     2881 gcgttcgcct tggcgaaggc atcgatggcg ccctgggtga gctggagctg gcccgccaca
     2941 tccaccacgt tcaatgtaac aggtgaagat ggaagggcag gagcagacgc cgcaaacgca
     3001 cggcccgcac ccagagctgc caacccggcc atgccgccga gcagtgtcct gcggtcaacc
     3061 ttcggggtgg tcatggtcgt ttctctccca atctgagtgg tccgcttcgg ttcgccgcgt
     3121 acaagactgg ctggaacaag gcaacccgca ttgatctgaa tcaagtgcgg aacggagggc
     3181 gacagaaagt tgttgcttat caattgctaa accccctcat cgggagcggt attccaagcg
     3241 ccgcgcttaa cctgtttagg tcgcgcccgc cgggccgcga ccgctgtcgt atcaacaacg
     3301 cgatcgcgca atgccttgct tctattgatc cttgctggcc actgcggcag ggtgagggca
     3361 tgtccggacc tgcgcgcgag acccttgacc gacttcgact ggtcagcacc gccacgctga
     3421 ccacgcaatt gttcaagcgt ggactgcgca atgtcttcat gcagggtgta cagccgctca
     3481 gccgttacga tagcaacctg gtcggccctg cctttaccct
//
LOCUS       DATGEM010000602         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_11594, whole genome shotgun sequence.
ACCESSION   DATGEM010000602 DATGEM010000000
VERSION     DATGEM010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11594"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(495..1679)
                     /locus_tag="VKQ27_18445"
     CDS             complement(495..1679)
                     /locus_tag="VKQ27_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019092214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HLY90969.1"
                     /translation="MLAGLELADIFRRHGNAYRHAHDGHLGRVERRVMSAIELCRTAE
                     LGGHSEVCTSCGLIRCAYNSCRNRHCPKCQGQARADWLAARQAELLPVPYFHVVFTLP
                     APVAEIAFQNKATIYAILFRAAAETLRTIAADPKHLGAEIGVVAVLHTWGQNLHHHPH
                     LHCVVPGGGPSLDGTRWIACRPGFFLPVRVLSRLFRRLFLESLRAAFATGELGFFGEL
                     AELAEPAAFARRLAELRRIEWVVYAKPPFGGPEQVLAYLGRYTHRVAISNSRLISLTD
                     GRVGFRWKDYRHHGKSKVMTLHADEFIRRFLLHTLPDGFHRIRHYGLLANGHRAEKLA
                     LCRKLLAVAAPTENPEPVRHHHEKAGMAFDLCPCCGGTMITLAPLPRPPPSHPSFWSD
                     SS"
     gene            complement(1707..2567)
                     /locus_tag="VKQ27_18450"
     CDS             complement(1707..2567)
                     /locus_tag="VKQ27_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY90970.1"
                     /translation="MAEISPLRRRMIEDMTVRNLSPATQRSYVHAVAKFSRFFGRSPD
                     RLGLEDVRAFQVHLVSTGISWPALNQTVCALRFFYGVTLGHGEIPERIPYAREPRKLP
                     VVLSADEVVRFLEAVPSLKTRAALTTAYAAGLRASEAVGLRLADIDSGRMVIRVEHGK
                     GGKDRYVMLSAQLLGILRTYWRLARPKTWLFPGREESKPIDVQVLYSACRSARAAAGL
                     AKRVTVHTLRHSFATHLLENGTDIRIIQVLLGHNNLSSTARYTRVSSGLIRQTKSPLD
                     GLTLEVVPPA"
     gene            complement(2698..3663)
                     /locus_tag="VKQ27_18455"
     CDS             complement(2698..3663)
                     /locus_tag="VKQ27_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HLY90971.1"
                     /translation="MGQVLHGSARTTAALRRAIQHSQASIRTLAERYGINPKTVAKWK
                     RRTHVADAPMGPKDPGSTILSKEEEAVIVAFRKHTLLPLDDCLYALQSTIPRLTRSSL
                     HRCLQRNGISRLPEVDGDKPRRSTFKTYPIGFFHIDIAEVRTEEGKLYLFVAIDRTSK
                     FAYAELHDRANRHTATTFLKALLEAVPYKVHTVLTDNGIQFCDLPRHRSGPTARWRIH
                     MFDLMCRQHGIEHRLTKPHHPWTNGQVERMNRTLKEATVQRYHYASHDQLKAHLATFL
                     MAYNFAKRLKTLRGLTPHEYICKIRTQSPERFRLNPLHHTSGLHN"
     gene            complement(4116..4364)
                     /locus_tag="VKQ27_18460"
     CDS             complement(4116..4364)
                     /locus_tag="VKQ27_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HLY90972.1"
                     /translation="MGILAWLILGLVAGFIASKVVNKTGEGLVMDLVLGVIGAIVGGW
                     IMSFFGEAGVNGLNLYSLVVAVLGAIVVLVAYHAIRRV"
BASE COUNT          812 a         1393 c         1465 g          792 t
ORIGIN      
        1 gacacggtgc gctccgcgcc gacctcgcgg cagcaacggc tcgatccgag cccagtccgc
       61 ctcgctcagc caataaaggc tccccattca agccctccat cactggaatg cttgaatcac
      121 atccctacct ccacgggaat ctggaggctt ctgtgaggtc gcgcgacgag gtcacggcag
      181 ccgggccgca gcgcgcgacc tcattgaagc cggattgagc gaacccgcgg gatggcgggc
      241 gcgctatggg gattgtagtt gtggcgcctg gtccgaggcg ccggtgagca catgctgtag
      301 cgtcatcagc acgagttggg tggccgcggt gacgtcaggc gggcgaccgt gacgctggtg
      361 gggaccgcaa ggtgtgatag ctctatcggc ggcgagccag agtgcgtgct ccagggaagg
      421 ctgtcggaca aagcgatccg cgccgagacg catcggcggc gtcggcacgc cggcgatgga
      481 agagtgccgg cggctcatga gctgtcggac caaaaggatg ggtgagacgg aggtggtcgc
      541 ggcagcggag cgagcgtgat catcgtcccg ccgcaacacg gacagagatc gaaagccatc
      601 ccggccttct cgtgatggtg tcggaccggc tcgggattct cagtgggcgc cgccacggca
      661 aggagcttgc ggcagagagc gagcttctcg gcacgatggc cgttggcgag aagaccgtaa
      721 tgccggatgc gatggaaccc gtcgggtaag gtgtgaagca agaagcgccg gatgaactcg
      781 tcggcgtgga gggtcatcac cttcgacttg ccgtggtgac gatagtcctt ccagcggaag
      841 ccgacccgac cgtcggtcag gctgatcagg cgcgagttgg agatcgcgac gcgatgcgta
      901 tagcgcccga gataggccag cacctgttcg ggtccgccaa agggtggttt ggcatagacg
      961 acccactcga tgcgacggag ttcggcgagg cgccgggcga aggcggccgg ctccgcgagt
     1021 tccgcgagct ccccgaagaa gcctaactcg ccggtagcga acgccgctcg caggctctcc
     1081 agaaacagcc ggcggaagag ccgggagagc acgcggaccg gcaggaagaa gccgggccgg
     1141 caagcgatcc atcgtgtgcc gtcgagcgac ggaccgccgc cgggcaccac gcaatggagg
     1201 tggggatggt gatgcagatt ctgaccccag gtgtgcagca ccgcgacgac gccgatctcg
     1261 gcgccgagat gcttgggatc agccgcaatc gtccgcagcg tctcggccgc cgccctgaac
     1321 aggatggcat agatcgtcgc cttgttctgg aacgcgatct cggcgacggg cgccggcagc
     1381 gtgaagacga catggaagta cggtaccggc agcaactcgg cctgccgggc agcgagccag
     1441 tcggcgcgcg cctgcccttg gcacttcggg cagtggcggt tccggcagga gttgtaggcg
     1501 cagcggatga gcccgcacga cgtgcagacc tcggaatgac cgcccagttc cgccgttcgg
     1561 cacagctcga tcgcgctcat cacgcgccgc tcgacacgcc cgagatgccc atcgtgggcg
     1621 tgacgatagg cgttgccgtg gcggcggaag atatccgcca actccaggcc cgctaacatg
     1681 gtgccgcctc cccgcgaagg cggggatcag gcgggcggca ctacctccag cgtcaggccg
     1741 tcgagtgggc ttttcgtctg ccggatgagg ccgctcgata cccgggtata acgagccgtg
     1801 ctcgacagat tgttatgccc gagcagtacc tggatgatgc ggatgtcggt gccgttctcc
     1861 agcagatgcg tggcgaagct gtgccgcagc gtgtgcacgg tcacccgctt cgccaaaccg
     1921 gccgcggcac gggccgagcg gcaggccgaa tacagcacct gcacgtcgat cggcttgctc
     1981 tcctcccggc cgggaaagag ccaggtcttt ggccgggcca agcgccaata ggtccgcaga
     2041 ataccgagca gttgcgccga cagcatgacg tagcggtcct tcccgccctt gccgtgctcg
     2101 acccggatga ccatccggcc gctgtcgata tcggccagcc tgaggccgac cgcctcggat
     2161 gcgcgcagcc ccgccgcgta ggctgtcgtc agcgcggcac gcgtcttcag gctcggcacc
     2221 gcttccagaa accgcaccac ctcgtcggcg ctcagcacca ccggcagctt gcgtggctcc
     2281 cgcgcatagg ggatccgctc ggggatctct ccgtggccga gcgtgacacc atagaaaaac
     2341 cgcagagcgc agaccgtctg gttcagcgcc ggccacgaga tccccgttga tacgagatgc
     2401 acctgaaaag ctcgcacgtc ttccagaccg agccggtccg gcgagcggcc gaagaaacga
     2461 ctgaacttcg ccaccgcgtg cacgtatgat cgctgtgtcg ccggcgacag attgcggact
     2521 gtcatgtcct cgatcatgcg gcggcgcaga gggcttatct cggccatcgg ggcatctcct
     2581 gtctgatgga ttgggcttcg acaaccgcaa tcctcccaga cgggggacct cccgcgcgag
     2641 acctcgccta aatcccgcgc aagcgggttc gttcaatccc ctaattgagt acggacccta
     2701 gttgtgtagt cccgaggtgt gatggagcgg attgagtctg aatctttcag ggctctgtgt
     2761 ccggattttg cagatgtact cgtgaggcgt gaggccgcga agtgtcttca gacgcttcgc
     2821 gaagttgtag gccatcagaa aggtggccag atgcgccttc aactgatcgt ggctggcgta
     2881 gtggtagcgc tggacggtgg cctccttcag ggttcggttc atccgttcga cctggccgtt
     2941 cgtccagggg tggtggggct ttgtcagcct gtgctcgatg ccatgttgcc ggcacatgag
     3001 gtcgaacatg tggatccgcc acctcgcggt cggtcccgat cggtgccgcg gcagatcgca
     3061 gaactggatg ccgttgtcgg tcaggaccgt gtggaccttg tacggcacgg cctcgaggag
     3121 agccttgaga aacgtggtgg cggtatgccg gttcgcccgg tcgtgcagct cggcataggc
     3181 aaacttcgag gtgcgatcga tggcgacgaa aaggtagagc ttgccttcct cggtccgaac
     3241 ctccgcgatg tcgatgtgaa aaaagccgat cgggtaggtc ttgaacgtgc tccgcctcgg
     3301 cttatcgcca tcgacctccg gcaatcggct gatcccgtta cgctgcaagc agcggtgcaa
     3361 gcttgagcgc gtcaggcggg ggatcgtgct ctgcagggcg tagaggcaat cgtcaagcgg
     3421 caggagggta tgcttgcgga aggcaacgat gactgcctct tcctccttgc tcaatatcgt
     3481 cgagccaggg tccttgggcc ccatcggcgc gtcggcgaca tgggtgcgtc gcttccactt
     3541 cgccacggtc ttcggattga tgccgtagcg ctcggccagc gttcttatgc tcgcttgact
     3601 atgctgtatt gctcgacgga gcgccgccgt cgtgcgggcg ctgccgtgta gaacctgtcc
     3661 cataggtccc ccttgtcgat ggcaccagaa accgtaccat cacactgcgg gactaaacac
     3721 ctagtccgga gcaccttccg attaagcgga atcggagcgt gctctagatt cttggcttga
     3781 ccgcattctc ggcgccgaac cggcgtccac ttcgagcaga aatgggggac gggcctggac
     3841 ggaccccgac tacagacccg ggagtggtca tcggcgcctc aggcgctgga aggcttcggg
     3901 aatccttccg gttctgaggt aggtcgactt catcgagcgc ctgacgagct atccgcgcta
     3961 ccgctcggag taggcgccaa cttgctactg cttcggcccg atgcgcaaca gccgcgcggc
     4021 cgacgcggat gccagacgcc aaccgcgcaa cgtctttagg cccgctgagc gcggtcccct
     4081 gtcgccggta tcgtgcgcgg gagtcctttg actggctata ctcgccgaat cgcgtgatac
     4141 gcgacgagca ccacgatcgc gcccagcacc gcgacgacaa ggctgtagag attgaggccg
     4201 ttcacaccgg cttcgccgaa gaacgacatg atccagccgc cgacgattgc accgatcacc
     4261 ccgagcacca gatccatcac caatccctcg ccggtcttat ttacgacctt gcttgcgatg
     4321 aacccggcga cgagtcccag gatcagccac gccagaatgc ccattgtttt ccccttgctt
     4381 ggtttgtggt ttgaaacgga atcgttgatc ttacgctagg tccaccacac tgatcacgcg
     4441 gcgatcctaa tcagacatcc ag
//
LOCUS       DATGEM010000603         6310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1180651, whole genome shotgun sequence.
ACCESSION   DATGEM010000603 DATGEM010000000
VERSION     DATGEM010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180651"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1352
                     /locus_tag="VKQ27_18465"
     CDS             408..1352
                     /locus_tag="VKQ27_18465"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLY90973.1"
                     /translation="MFADLVQQVDTAPLAGSVYTRQRDGIEYIYAKLPAGRARVDRFI
                     GKSGDPAAEAQAASLRRGGELARSRREIISILKGHRLASPDRALGAALDAIAFAGLFK
                     QGAVLVGTGAYMMFEPLVGSRLPAPTLMTGDLDLATAQLALTADPPERMEDILRRADA
                     TYEGIPQLDPRQPPSRFRNAEGYLVDLLTPTRSRKDQNPMPLKALEAGAAPLHYLDWL
                     ISEPIPTVALWGSGIALQVPQPAKFAVHKLILAQRRDPANRHKRAKDLDQARALIDAL
                     LVHDRFVIEDMLADARGRGRAGWAEPIARSLEEIGTPI"
     gene            1530..2201
                     /locus_tag="VKQ27_18470"
     CDS             1530..2201
                     /locus_tag="VKQ27_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLY90974.1"
                     /translation="MRAIFIRHGQSTGNAGIPCTDLASIALTELGWEQARAIAAGWVE
                     APTLIVTSPYLRTQQTARPTIDRFPDVPVETWPIEEFTYLQPARWNGTSSAERMPHLE
                     RYWGEADPGYCDGEGAESFATLLGRADAALARLAALPGDALAYVFSHGQFIQAVRAIV
                     VDTGLDERGKMLRFWRKGEPPAISNAERLPLHWSGVEWRPLADPDPVAELPGDAEVSQ
                     FIRKR"
     gene            2219..2386
                     /locus_tag="VKQ27_18475"
     CDS             2219..2386
                     /locus_tag="VKQ27_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90975.1"
                     /translation="MDLFEFVAIGPLDDEAAPDALRLPIEREGELIDEDALAELDVSF
                     FQVGFDRPTPA"
     gene            2405..2584
                     /locus_tag="VKQ27_18480"
     CDS             2405..2584
                     /locus_tag="VKQ27_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90976.1"
                     /translation="MASLGPGPHRSGNVADARDVKVSGVAPTRNGLITKGMRYGPAHG
                     DTAFTVPLFDAYMGV"
     gene            2722..2994
                     /locus_tag="VKQ27_18485"
     CDS             2722..2994
                     /locus_tag="VKQ27_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HLY90977.1"
                     /translation="METSSVLAEPVELVAIDPARNIRRRYRITVARDLFGHVLVETSW
                     GRIGRAGQSCTYSFPDETRAAAFVRTTLRRRAGAPKRIGVAYCARR"
     gene            2979..3530
                     /locus_tag="VKQ27_18490"
     CDS             2979..3530
                     /locus_tag="VKQ27_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90978.1"
                     /translation="MRSPLIAHRPYQQVGLAAEGEGMTIVAIEAPANRSLPGILVPVL
                     RAALLRLDRMKQASAGVRRALRALAGFAKLKVKYDDPEVALDFEPEPGLADSGDLDAD
                     LADLTPAEKRYLQAMATLGPGPHRSGDVGEALGVKVSRVAPPRNSLITKGMFYSPAHG
                     DTAFTVPLFDAYMSRVMPDHRDD"
     gene            complement(3583..3885)
                     /locus_tag="VKQ27_18495"
     CDS             complement(3583..3885)
                     /locus_tag="VKQ27_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HLY90979.1"
                     /translation="MARLDVHARRDGGPGYLLDVQADLLSSLNTRLVVPLLPAGQAPK
                     PAARLNPLFEIAGDPVVMVTQFSSAVPIRELGDRVATLADRHETVMAALDMLITGV"
     gene            complement(3885..4139)
                     /locus_tag="VKQ27_18500"
     CDS             complement(3885..4139)
                     /locus_tag="VKQ27_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HLY90980.1"
                     /translation="MALRGSTAAGDRPRRATNVSLDPRLIEEARALDVNVSRACEQGL
                     IEQIAEARAARWLADNADALAASNAYVEAQGLPLASVRLF"
     gene            complement(4171..4815)
                     /locus_tag="VKQ27_18505"
     CDS             complement(4171..4815)
                     /locus_tag="VKQ27_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HLY90981.1"
                     /translation="MLIGYIRVSKADGSQTLAPQRDALLAAGVDPSRLYEDLASGRHD
                     ARPGLTACLKALQPGNTLVVWKLDRLGRNLRHLVTTAEALRERGVGLKVLAGAGAQID
                     TTTANGRLAFGIFAAFAEFERELIAERTQAGLAAARARGRLGGRPRKMDRATLAMAMA
                     AMADRNAVATDVARRLGITTTTLYAYVNGDGSPKAAGQALLDGTLPRKERSADA"
     gene            5204..6031
                     /locus_tag="VKQ27_18510"
     CDS             5204..6031
                     /locus_tag="VKQ27_18510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY90982.1"
                     /translation="MTLLVRDAEAILRENLEFHLRQGIDYFIITDNCSVDGTVGIVRE
                     YAERGCAELILEPRDDFSQWRWVTRMARRAATHHGADWVVNNDDDEFWFAHSGRLRDA
                     LERTPDSCDGLVVERYNHPPIADHGGREFLEAMIYRECRSLNALGHLLPPKVCHRAFA
                     DIVVAQGNHMASRESGPLDIRPGPDFSISHFPIRDFASFERKIVNGGAAYARNTELSP
                     DVGGTWRWLYSIWQEGRLRAWYEDQLLTPAKIAEGLARRSLVPDDSVLRALRLGAGA"
     gene            6015..>6310
                     /locus_tag="VKQ27_18515"
     CDS             6015..>6310
                     /locus_tag="VKQ27_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90983.1"
                     /translation="MERVRNMDRRGIVTLCDQNYFEGLMQLHASIQTSWACPVLCYDI
                     GLSPAQRLQAAETQGLTILDLPADPLIGELERATSDCTPLAKPGKRIWPLWICPL"
BASE COUNT         1102 a         2023 c         2076 g         1109 t
ORIGIN      
        1 tcgtcaggct cgagcatcgc ttctctccgc gcaaccaccg cggagatcct ctcatcacac
       61 gtgctcatct gtcccttcct cctctcagag ggggtcagat ttcactggcg acgggggacc
      121 agaattgcat gtcgcctgac atcggccaca taggattttc gagggttccc caccggagcc
      181 ctgcctccgc ctcacatgat gtgtctccgg tcgatgggat agttttacat ttcgcagtcg
      241 gtccagtccc ctcagatatc cagctgagcc tggtcacgag taccggcgga tatgcctcgc
      301 ctgtacagac gaagaatgaa aaatccgtct gtacattgct cgtacagacg ggatgtagta
      361 aacccgtctg tacaatgagc atgtcccttc agctctccat ccgaactatg ttcgccgacc
      421 tcgtgcaaca ggtcgacacg gcgccgctgg ccggctcggt ctacacgcgg caacgcgacg
      481 gcatcgaata tatctacgct aagctgccgg ccggccgggc gcgggtggat cgattcatcg
      541 gcaaatccgg cgatccggcg gctgaagccc aagccgcctc gctccgacgg ggtggcgagt
      601 tggcgagaag ccgtcgcgaa atcatatcga tcctcaaagg gcacaggctc gccagtcccg
      661 accgggcgct cggtgccgct ctcgacgcca tcgcttttgc ggggctgttc aagcaggggg
      721 ccgttctggt ggggacgggc gcctacatga tgttcgaacc tctggttggc agccggcttc
      781 ccgcgccgac gctgatgacc ggcgatctcg atctggccac ggcccaactg gcgctgaccg
      841 cggacccgcc ggagcgaatg gaagacatcc tgcggcgtgc cgatgcgacc tatgaaggca
      901 ttccgcagct cgatccgcgc caaccgccat cccgcttccg caatgccgag ggctatctgg
      961 tcgatctgct gacaccgacg cgaagccgga aggaccagaa cccgatgccg ctgaaagccc
     1021 tggaggcagg cgctgcaccg cttcactatc tggactggct gatcagcgag ccgataccca
     1081 cggtcgcgct atggggttcg ggcattgcct tgcaggtgcc ccagccggcg aagttcgccg
     1141 tccacaagct catcctggcc cagcgccgtg atcccgccaa tcgccataag cgagcaaagg
     1201 atctggatca ggcccgggcg ttgatcgacg cgcttctggt ccatgaccgc tttgttatcg
     1261 aggacatgct ggccgatgcc cgggggaggg ggcgtgccgg ctgggccgag ccgatcgcaa
     1321 gatccctgga ggagatcggc actccgatat gaggtgcgaa cggcggctgc tcgacagcat
     1381 cgacatcacc acgccggccg gcctgcggga ccgggtgctg ccgcgatggc gaaccctgct
     1441 tccacccgca ccacacagct ctatgatcgg cgctcggatg cggtgacgct cgacgagatc
     1501 gagcgggttt ggatcaaggg agtgtcagcg tgcgtgcgat cttcatccgg catggccaga
     1561 gcaccggcaa cgccggcatt ccctgcacgg acctcgcttc gatcgcgctc accgagctcg
     1621 gctgggaaca ggcccgcgcg attgcggccg gctgggttga agccccgacg ctgatcgtca
     1681 cctcgcccta tctgcgcacc cagcagaccg cccggccgac catcgatcgc ttccccgatg
     1741 tcccggtgga gacctggccg atcgaggagt tcacctatct ccagcccgcc cgctggaacg
     1801 gcacgagcag cgccgaacgc atgccgcatc tggaacgtta ttggggggag gctgacccgg
     1861 gctattgcga cggggagggg gcggagagct tcgcgaccct gctcggccgg gccgatgcgg
     1921 cactggcgcg gctggcggcg ctccctggcg atgcgctcgc ttatgtgttc agccacgggc
     1981 agttcatcca ggcggtgcgc gcgatcgtgg tcgacaccgg cctcgatgag cggggcaaga
     2041 tgctccgctt ctggcgcaag ggggagccac ccgcgatcag caacgccgag aggttgccgc
     2101 ttcactggtc gggcgtggag tggcggcctc tggctgatcc ggatcctgtg gcggaactgc
     2161 ccggtgatgc ggaagtcagc cagtttataa ggaaacgata aacctccata acttcgctat
     2221 ggatctgttc gagttcgtag caatcggtcc gctcgatgac gaagccgcgc ccgacgcgct
     2281 ccggctgccg atcgagcggg aaggggagct gatcgacgag gacgcgctgg cggaactgga
     2341 cgtgagcttc ttccaagtcg gcttcgatcg gccgactccc gcctagaagc gctatctgca
     2401 ggcgatggcg tcgctcgggc cggggccgca tcgttccggt aatgtggcgg atgcgcggga
     2461 cgtcaaggtg tccggcgtgg cgccgacgcg caacggcctg atcaccaagg gcatgcgcta
     2521 cggccccgcg catggcgaca ccgcgttcac cgtgccgctg ttcgacgcct atatgggcgt
     2581 gtgatgtcgt agcggatagc agccgcccgc gcgctggcgc cggtccggaa ggaggtcttg
     2641 atcgaccgcc tgattcagtc gcgccgcagt gagtcaggaa atccgtcgga caggcggcat
     2701 ggcggtcgat atcgtcgaac catggaaaca tcgtccgtcc tggccgagcc ggtcgaactt
     2761 gtcgcgatcg atccggcccg caacatccgt cgtcgctatc ggatcacggt cgcccgggat
     2821 ctgttcggcc atgtgctggt cgaaacaagc tgggggcgga tcggccgtgc cggccaaagc
     2881 tgtacataca gcttcccgga cgaaacgagg gcggccgcct tcgtccggac gactctccgc
     2941 cgtcgcgccg gcgcaccgaa acggatcggt gtcgcctatt gcgctcgccg ctgattgccc
     3001 atcgccctta tcagcaggtc ggtctcgcgg ccgagggcga ggggatgacc atcgtggcga
     3061 tcgaggcgcc cgcgaaccgc tcgctgccgg gcatcctcgt gccggtgctc agggccgccc
     3121 tgctccggct ggaccgtatg aagcaggcct ccgccggagt tcgtcgcgcg ctgcgagccc
     3181 tggcaggctt cgccaagctc aaggtcaaat atgatgatcc cgaggtcgcg ctcgatttcg
     3241 agcccgagcc cggcctcgcc gacagcggcg acctcgacgc cgatctcgcc gacctgacgc
     3301 cggccgagaa gcgctatctg caggcgatgg cgacgctggg gccggggccg catcgatcgg
     3361 gcgacgtggg ggaggcgctg ggcgtcaagg tgtccagagt ggcgccgccc cggaacagcc
     3421 tgatcaccaa gggcatgttc tacagccccg cgcacggcga caccgcgttc accgtgccgc
     3481 tgttcgacgc ctatatgagc agggtgatgc ccgatcaccg ggacgattga gggcgctttt
     3541 cgtccgcgag agaaagaagc ggagggggcg agctcgaacg gctcacacgc cggtgatcag
     3601 catgtcgagc gccgccatca ccgtctcgtg ccgatccgcc agcgtcgcca cgcggtcacc
     3661 cagctcgcgg atcgggacgg cggatgagaa ctgggtgacc atcaccacgg ggtcgcccgc
     3721 aatctcgaac agcggattga gccgcgcggc cggtttcggt gcctgtcccg cagggagcag
     3781 cggcaccacc agacgggtgt tgaggctgga gagcaggtcc gcctgcacgt cgagcagata
     3841 gcccgggccg ccatcccggc gggcgtggac gtcgagccgg gccatcagaa caggcggacg
     3901 gatgccagcg gcagaccctg cgcctcgaca taggcattgg acgcggcaag ggcgtcggca
     3961 ttgtcggcca gccagcgcgc cgcgcgcgcc tcggcaatct gctcgatcag gccctgctcg
     4021 caggcgcggg aaacattgac gtcaagcgcg cgcgcttctt cgatcagcct cgggtcgagc
     4081 gagacgttgg tcgccctgcg ggggcgatcc cctgcggcgg tggatcctcg aagagccatg
     4141 cgcataatct atgcgcaccc cgacgaggga tcaagcatcg gccgatcgct cctttcgggg
     4201 cagagtgcca tcgagcagcg cctgccccgc ggccttgggg ctgccgtcgc cgttgacata
     4261 ggcatagagt gtcgtggtcg tgatgccgag gcgccgggcc acgtcggtcg cgaccgcgtt
     4321 gcgatcggcc atcgccgcca ttgccatggc gagcgtcgcg cggtccatct tgcgtggtcg
     4381 gccgccgagc cgtccccgcg cgcgcgccgc ggcgaggccg gcttgcgtcc gctcggcgat
     4441 cagttcgcgc tcgaactcgg cgaaggccgc aaagatgccg aaggcgagcc ggccgttggc
     4501 ggtggtcgta tcgatctgcg cgccggcgcc ggccagcacc ttcaacccga cgccgcgctc
     4561 gcgcagcgct tcggccgtcg tgacgagatg gcgcaggttc cgcccgagac ggtcgagctt
     4621 ccagacgacc agcgtgttgc cgggctgcag cgccttcagg caggcggtga ggccgggccg
     4681 ggcatcatgt cgccccgacg cgagatcctc gtaaagccga gagggatcca cgcctgcggc
     4741 cagtagcgca tcgcgctggg gagcgagcgt ctgtgagccg tccgccttcg acacccggat
     4801 atagccgatg agcaagccga cccccgtcag aaaagatggt tgtgaccatg caacaggaca
     4861 ttgattttcg caacggcttt acgagcacgc ggcgcggcgg tcggggatgg cgatccccga
     4921 ccgccagaaa accgaccgtt attcttgtgc cggaagcatg atgttgtcgc aggaagactg
     4981 atccaatctc gttgctcttt cgcatcgaca gtatggctgg atgcctataa tctgccgctc
     5041 tagtcgtcat tttcatgaaa tgatggggta taatgatgag catatcgtga gagtaggcaa
     5101 aagccaaaga atgtgctgaa acgcccaatg attgaaatat gagaaatatt tcaatttatt
     5161 gtggtttcac cgcatcgaaa ggtatggcgc cgtgaagttg gtaatgacat tgctggtcag
     5221 ggacgctgag gccattctcc gcgagaatct ggaattccat ctccgccagg gaatcgacta
     5281 cttcatcatc accgacaatt gttcggtcga cggaacggtc ggcatcgtcc gggaatatgc
     5341 tgagcgcggg tgcgccgagt tgatcctgga gcctcgggac gatttcagcc agtggcgctg
     5401 ggtcacccgg atggcgcgac gggcggccac gcatcatggc gccgactggg tcgtgaacaa
     5461 tgacgacgac gaattctggt tcgcgcatag cggccgtttg cgcgatgcgc tcgaacggac
     5521 gccggactcc tgcgacgggc tcgtcgtcga gcgctataat cacccgccga tcgccgatca
     5581 tggcggacgg gaatttctcg aggcgatgat ctaccgcgag tgccggtcgc tgaatgcgct
     5641 ggggcacttg ctgcccccca aggtctgcca tcgcgccttc gcggacatcg tcgtcgcgca
     5701 aggcaatcat atggcgagcc gcgagagcgg tcctctcgac atccggccgg gaccggattt
     5761 cagcatttcc catttcccga tccgggattt tgcgagcttc gagcgcaaga tcgtgaacgg
     5821 aggcgcggcc tacgcccgca ataccgagct cagccccgat gtcgggggga cctggcgctg
     5881 gctctattcg atctggcagg agggaaggct ccgcgcctgg tacgaagacc aattgctgac
     5941 gccagccaaa attgccgagg gcctcgcgag acgctcgctg gtgcccgatg actcggtgct
     6001 gcgcgcgctg cggcttggag cgggtgcgta atatggatcg ccgggggatc gtcactctgt
     6061 gcgatcagaa ttatttcgaa ggcctgatgc agctccatgc atcgatccag accagttggg
     6121 cctgtccggt cctttgttac gacataggcc tgtcgcctgc gcagaggctt caggcggccg
     6181 agacgcaagg cctcaccatt ctcgatctgc ccgccgaccc cctcatcggc gaactcgaac
     6241 gggcgacgag cgattgcacg cctctggcca agcccggcaa gcggatctgg ccgctttgga
     6301 tctgccctct
//
LOCUS       DATGEM010000604        11593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_963207, whole genome shotgun sequence.
ACCESSION   DATGEM010000604 DATGEM010000000
VERSION     DATGEM010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963207"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VKQ27_18520"
     CDS             <1..1127
                     /locus_tag="VKQ27_18520"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY90984.1"
                     /translation="HALDYDDVSGVMGGHPSVMLIPPMLALAELIGATGRDLALAYVV
                     GYETACRIARAVHFHHYDKGWHPTATLGIFGTVAAAARLLHFTHDQTATAIGLAASFA
                     SGLKANFGTMTKPLHVGHGTRNGLFAALMVREGFTANPAALEAKQGFLDVFNGHGTYN
                     VERMLAEWYAPLEVEGGGEPGLKPYPCCGSAHSSINRMIHLARTHDLLPEQVETIEIM
                     PHPRRLPHTNNPDPRTPLGAKFSIQYVVARALADRAVRLEHFEGDAPLDPAVRDLMAR
                     TTARPHPDMAEDAPLQWGAEVVVTTRDGQRFASRLDDFERRGPGGQPMTAEELWEKFS
                     DCARRSLPGGKIAPLFDRLNHIETLAAITDLMPLLEGDAE"
     gene            1124..1588
                     /locus_tag="VKQ27_18525"
     CDS             1124..1588
                     /locus_tag="VKQ27_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY90985.1"
                     /translation="MSADERLAALGLTLPSPPKPIGNYVPFRLAGNLLFLSGVGPRRA
                     DETMMTGKVGAEVSVEQAYEAAKWCGINLLVNMMAAVGTLERVDTVLKVLGMVNAVPD
                     FTRHPEVINGCTDLFVEVFGEGGRPARSAVGMGSLPRNISVEVEAIVLVRGT"
     gene            complement(2038..3111)
                     /locus_tag="VKQ27_18530"
     CDS             complement(2038..3111)
                     /locus_tag="VKQ27_18530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY90986.1"
                     /translation="MPPRVDVNMMVYNCVHTVAAAIDSVRAQTWPEVTLTLIDNASTD
                     GTMAVLEDYAARHSGVRIRRNRCNAGPIANIQRAFWWGDADFVMPKTGDDLIAPDYVA
                     RLMEVLLTHPDTAMCHAGGEVFTAAGKVIHVYPPEHWLHAVGPDPVERARAVMWHYTS
                     APSFWGVYRRDAVDQLSTIRYRPGFDHAVVAELALYGEIRHVPEVLYRRRGGGKPVML
                     IGRGATEAGNRNVPLDDDLAEQRWRAPLITTAHTHLEVFAIARLSQAQRATLMRSAYE
                     IFRARWLPLFQREADQLRAALPELLERIATAEPIPAAWLTRSVGDVLLAAQTMLPEAG
                     FSGDLLSLAALSDEVSASEAPVC"
     gene            complement(3095..5338)
                     /locus_tag="VKQ27_18535"
     CDS             complement(3095..5338)
                     /locus_tag="VKQ27_18535"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90987.1"
                     /translation="MALLAAGRSADALDALRQAADLGEGSPSTLLNLAIAEDRAGDPV
                     RARGLMRELGQRLPDWDEPPLRLAESLRAHGDPAGAEAAYTQVLDINPRREEALVALA
                     GLLIARGAAASAQPLLLRCCGIAPDRAEAWDTLGISLVQTGDPALARTAFREALRRAP
                     DSLNYALHRVDAAIRAGAAADELARLDLNCDTDPLNAALRCAKGVLLEHLKRMPEAIE
                     SFEIAAALAPEAPLPAAFLGQALIRTVRLREGEAALRRAAQLDPTNATLRNDLAATLM
                     RMHRHGEARTLLQSALDEYGPDARILCNLENTTCCLGLHEEAVALARRAIALAPDDLN
                     PRRSLLSALVYSDGVPGSAITDAARTCAERIPRATPTPFTNAREPERKLTIGLLSGLL
                     KTHPVGWLTIAGLEALDPAAFAVVALAHNTWSDMLARRFRAIAQQWEDISTLNDEAVA
                     HKARECGIDILIDLGGYGEGSRISACAQRLAPVQVKWVGMQSCTTGMPDIDWMLTDRW
                     ETPPELAHLYTERPLVMPDGYVCYSPPPYAPDVTAVPALANGCVTFGCFNNLAKVTQR
                     VVATWARVLQRVAGSRMVLKTHQFTDAETRDRVRAAFAAHGVGDDRLELRGPSDHRTF
                     MGEYNGIDIVLDPFPYSGGLTTCEALWMGVPTVTLAGEIFAARHSVSHLSNAGLADWV
                     ATDIETYVELAVAKASDIAALASLRSGLRAQVRASPLCDGPRFGRNLGAVLRVAWQDW
                     CEHAAAR"
     gene            complement(5442..6881)
                     /locus_tag="VKQ27_18540"
     CDS             complement(5442..6881)
                     /locus_tag="VKQ27_18540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLY90988.1"
                     /translation="MHTARANSQPDILLRRAVSLMDDGHVGAARALLAAVRRMAPDLP
                     GQIELTVRLALREGQIELARAELDPAIERAPDHAGLRKLRAELRRELGDIPGAAADAA
                     EAVVLNRDDPVAKALLGVLLLEMHRPDDAVACLREAVAAEPGNPAFREAFAAAADIAG
                     DADAALATLTEGIAAAPRYVSLRNAAVLHCIRRRDFAAAAAIAEEARSAGVVDACLFG
                     LKGHALSSLGRHEEAADAYTEALKLGPEDQYVRHLVAAAGVLPGASRAPIDYLRAVFN
                     GYAERFEAHLLSLGYRVPWLLHDALQVQGVHGGPVLDLGCGTGLLAVVCADLDLGPFV
                     GVDIAPGMLAQAAGKQLYADLHEADVLDFLRADTRTWPLILASDVLCYFGALDELFIA
                     VQRRLTPAGLFAFSVELLQPDAQGAMPGNGDWTLGRQGRFAHAEQYLVRAAEAAGLAV
                     RNVTPEILRCEADAPVLGLIVVLGLGNAH"
     gene            complement(6921..7259)
                     /locus_tag="VKQ27_18545"
     CDS             complement(6921..7259)
                     /locus_tag="VKQ27_18545"
                     /inference="COORDINATES: protein motif:HMM:NF018959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HLY90989.1"
                     /translation="MRTGARAYEAATSCRSVREQEADVFRRAVGALKLARDGEPVSQT
                     RAIADNRRLWLAVTDLMRDPRNELPDDLRASIVSVGLAVQREMDRPAPNFDFLISINE
                     HFAAGLSASG"
     gene            complement(7256..7723)
                     /locus_tag="VKQ27_18550"
     CDS             complement(7256..7723)
                     /locus_tag="VKQ27_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HLY90990.1"
                     /translation="MSNLVLELRQGEMMIVNGAPIRFRTKSRIELTAKARFLFGKQIM
                     PPDQADTPARRIYFALQTAYIGSEEERANGLVAARELIVAFKEATTSALACEILDRAL
                     AAAEADDCYQALKLARRIVRHEDSVLGRVAQPACVMTHDASATHRETAEPCRT"
     gene            complement(7799..8659)
                     /locus_tag="VKQ27_18555"
     CDS             complement(7799..8659)
                     /locus_tag="VKQ27_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HLY90991.1"
                     /translation="MSFLNSVNTNTGAMIALQALNKTNSQLAAAQKQVSTGFRVADST
                     DDGAAYAIAQNVRSTVGALGSANQQLGNVQGLLSTTQSSLNNISNTMTSMRDVLVKLS
                     DSSVTSTDRTNYIAQYNSLLSNVKTFIQDANYNGKTLVGDISGGSGTFARVAVVRNES
                     GASYAVATYSGSALFGSISYSSTQLNAATTVQGIIGTATTASFINKFNALNNELNTIG
                     SAQKYVSNQVSYNSDKIDALNTGIGALVDADLAQESAQLQALQIRQQLGTQALSLANQ
                     APQTLLSLFK"
     gene            complement(8769..9899)
                     /locus_tag="VKQ27_18560"
     CDS             complement(8769..9899)
                     /locus_tag="VKQ27_18560"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HLY90992.1"
                     /translation="MSGVISNVTGPSDYGMMNTLIAQSNTVKQKLDKLTIQASTGLIG
                     TTYAGLGTGAPTSLDLRPQIANLQTWQNNVDAATGRISVAQSSLGSIESIAANFYAQL
                     NNLQGVDPGEIDSIAANARDALKQVAGLLDTQDGGTYVFAGQDSGNPPVPDSDNILNS
                     GFYTQINAAVNGLAVNGAANTANATFAVAVSNAAGTSPFSTFLSQPYATVQAALPSVQ
                     VGQTHQETTGIPASANGLIPTSPPGQTTIVGGPVSSTGSYMRDVLRALATIGSLSSGQ
                     ANVQGFADLVQDTRISLNGAVTALNEDSGVLGNVQSSINATQTQLSDTQTALTTQVSS
                     VEDVDMAQTLSQLALVQTQMQASYQLIAGVSALSLVKFLPTG"
     gene            complement(9910..11379)
                     /gene="flgK"
                     /locus_tag="VKQ27_18565"
     CDS             complement(9910..11379)
                     /gene="flgK"
                     /locus_tag="VKQ27_18565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HLY90993.1"
                     /translation="MTIASALSIATGGLNNVNAQLALVSQNVANASTPGYAREIGTQH
                     DVVANGQGLGVHTGPAIRDVDLALQAQVFSQNGTVAGLQMRQSALQSIDAVMGTPGQD
                     TDIASLIANLQNQFSTLLNDPSSLAQQSQVVTSAATLAQNINTLSNAYGAQRQTAQDN
                     IVNEIGTLNTTLGTIGGLSNQIITLKASSQSTADLESQRDAAIATVSQLVSVKVLQQP
                     NGDVLLTTTAGLTLPIHGNAPPLSTSDVNVQSGTYYPGGGIPAILLGGTDVTRQLQGG
                     QIGANITLRDTTLPTYQGELDELSESMATRFQANGLTLFTDPTGTVPVAAAPPAQNGY
                     VGFAGTIQVNPAVLANPSMVRDGDMPPVPTQAGYTAIINAVLNNTLGGNPPLAANVTG
                     IGPSGTLNAPFSSPPTLGGFASTMLAAQAQESTTASSQLATEQSVQTMLNGKLTAQSG
                     VSIDTEMSNMIQLQNAYGANAKVIAAVQSMWNQLLGSVS"
     gene            complement(11387..>11593)
                     /locus_tag="VKQ27_18570"
     CDS             complement(11387..>11593)
                     /locus_tag="VKQ27_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HLY90994.1"
                     /translation="SSESGTPLAEAPGTNGAGTLVTESVEGSNVDIATEFSKLIVAQQ
                     AYSANTKMVTTADQMLQQTINMKQ"
BASE COUNT         1997 a         3720 c         3787 g         2089 t
ORIGIN      
        1 cgcacgcgtt ggattacgac gacgtgtccg gcgtgatggg cggccatccc tcggtgatgc
       61 tgatcccgcc gatgctggca ctcgccgaac tcatcggcgc taccggccgc gatctcgcgc
      121 tcgcctatgt cgtcggctac gaaacggcat gccgcatcgc tcgtgccgtg catttccacc
      181 actacgacaa aggctggcac cccaccgcga cgctcggcat cttcggcact gtcgccgcgg
      241 ccgcacgcct gctccatttc acccacgacc agaccgcgac ggcgatcggt ctcgccgcat
      301 cattcgcgtc cggcctgaag gccaacttcg gcaccatgac gaagccgctg catgtggggc
      361 acggcacgag gaacgggctg ttcgcggcgc tgatggtgcg cgagggcttc accgccaatc
      421 ccgccgcgtt ggaagcgaag caggggttcc tcgacgtgtt caatggccac ggtacctaca
      481 atgtcgaacg catgctggcc gagtggtatg cgccgctcga agtggaaggc ggtggcgagc
      541 cggggttgaa gccgtatcct tgctgcggct ccgcgcattc ctcgatcaac cgcatgatcc
      601 acctggcgcg cacgcacgat ctgttgccgg agcaggttga gacgatcgag atcatgccgc
      661 atccgcgccg cctgccgcac accaacaatc ccgatccgcg taccccgctc ggggcgaagt
      721 tcagcatcca gtatgtggtg gcccgggcac tggcggatcg cgccgtgcgg ctggaacact
      781 tcgagggcga tgcgccgctc gatcccgctg tccgagacct gatggcgcgc accaccgcac
      841 gaccgcatcc cgacatggca gaggatgctc ccctgcagtg gggcgcggag gtcgtggtga
      901 cgacacgcga tggccagcgc ttcgcctccc ggctggacga cttcgagcgc cgcggccccg
      961 gcggccagcc gatgacggcg gaggagctct gggagaaatt ttccgattgc gcccgccgct
     1021 cgctgcccgg gggaaaaatt gcgcccttgt tcgatcgcct gaaccacatc gagacactcg
     1081 ccgccatcac agatctgatg ccgttgctgg aaggagacgc cgaatgagtg ctgacgaacg
     1141 actggccgcg cttggcctga ccctgccatc gccgccgaag cccatcggca actacgtgcc
     1201 gttccggctc gccggcaacc tcctgttcct ctcaggggtc ggcccgcgtc gcgccgacga
     1261 gacgatgatg accggcaagg tgggcgccga ggtcagcgtc gagcaggcct acgaagccgc
     1321 gaaatggtgc ggcatcaact tgctggtgaa catgatggcc gcggttggca cgctggagcg
     1381 tgtagatacg gtgctgaagg tgctgggcat ggtgaacgct gtgcctgact tcacccgcca
     1441 tcccgaggtg atcaacggct gcaccgatct gttcgtggaa gtgttcggtg agggtggccg
     1501 tccggcgcgc tccgccgtcg ggatgggcag cctgccacgc aatatctcgg tggaggtgga
     1561 agccattgta ttggtgcgcg gcacttaact gcgttttaac cgcgccgggt gcatgatctg
     1621 cgcgtactgg gcgccagaag ggcgcggctg gagcggtcaa agccgccatg tggaatgacc
     1681 tgactgtccc gtctgtcact atgccggtcg ctgctgcggc gtccgctgct ctggcgaaag
     1741 gctctaccag tgtggccgtt gaactgccta aagcagcagc gccctcgttg gtagaaccga
     1801 acccgagcct gcagctggaa ccggcacttg gcctggttgt tattcagttt cgcgacgatg
     1861 ctggcgcggt gaccgactcg attccaagcg agcggcagct acaggcgtac cagcgctggg
     1921 cgacgacgca attcggaccg gcgccacgcg gcatgccgct gatgggcacc cgcagcggcg
     1981 cagtggcgcg gcaacatgtc gcagtggcca agcccgtggc agcaagggaa cccgcggtca
     2041 gcacaccggc gcctcgctcg ccgacacctc atcgctgagc gccgccagcg agagaaggtc
     2101 gccagagaag cccgcctcgg gaagcatcgt ttgggcggca agcagtacgt ccccgactga
     2161 gcgtgtcagc cacgccgccg gtatcggttc ggcggtggcg atacgctcca acaactcggg
     2221 gagtgcggcg cgcagctgat cggcctcccg ctgaaacaac ggcagccagc gggcccggaa
     2281 gatctcgtat gccgaacgca tcagtgtggc acgctgtgcc tgagacagac gtgcgatggc
     2341 gaacacctcc aggtgcgtgt gcgccgtcgt gatcagcgga gcgcgccagc gttgctcggc
     2401 caggtcatcg tccaacggca cgttccgatt gccggcttcg gtggcgccgc ggccaatcag
     2461 catgacgggc ttgccgccac cgcggcgccg atacagcacc tccggcacgt ggcggatttc
     2521 accatacagt gccagctccg cgaccacggc gtgatcgaac ccgggacggt aacgaattgt
     2581 ggacaactga tcgaccgcat cgcgccggta cacgccccag aaactgggcg cgctggtgta
     2641 gtgccacatc accgcgcgcg cgcgctcgac cggatcaggg ccgacagcat gcagccagtg
     2701 ctcgggcggg tacacgtgga tcaccttgcc ggcggcagtg aaaacctcgc cgccggcatg
     2761 gcacatcgcg gtgtcgggat gtgtcagcag cacctccatc agtcgcgcga cgtagtcggg
     2821 cgcgatcagg tcatcgccgg tcttcggcat cacgaagtcc gcatcacccc accagaacgc
     2881 gcgctggatg ttggcgatcg gaccagcgtt gcagcggttt cggcggatgc gcacgccgga
     2941 atggcgggcg gcgtagtcct ccagcacggc catcgtgcca tcggtcgagg cgttgtcgat
     3001 cagcgtcagc gtcacttccg gccaggtctg tgcccgcaca ctatcgatcg cggcagccac
     3061 cgtatggacg cagttataca ccatcatgtt gacatcaacg cgcggcggca tgctcgcacc
     3121 agtcctgcca agcgacacgg agaaccgcgc ccaggttgcg gccaaagcgt ggcccatcgc
     3181 aaagcgggct ggctcgtacc tgcgcgcgca gtccggagcg caacgaggcg agggccgcga
     3241 tgtcggacgc cttggccacc gccagttcga catatgtctc gatgtcggtc gcgacccagt
     3301 cagcgagacc ggcgttgctc aagtggctga ccgaatgccg cgcggcaaag atttcaccag
     3361 cgagcgtgac ggtcggaacg cccatccaca gcgcctcgca tgtggtcagg ccgccggaat
     3421 agggaaacgg atcaaggacg atgtctatgc cgttgtattc acccatgaag gtccggtgat
     3481 ccgatggacc gcgcaattcc agcctgtcgt cgccgactcc gtgcgcggca aacgcggcac
     3541 gcactctgtc cctggtttcg gcatcggtga actgatgcgt cttcagcacc atccgcgacc
     3601 cggctacgcg ctgcagcacg cgcgcccagg tcgcgaccac gcgttgcgtc accttcgcga
     3661 gattgttgaa acagccgaag gtgacgcaac cattggcgag agccggcact gcggtcacat
     3721 ctggggcata gggtggcgga ctgtagcaga cgtaaccatc cggcatgacc agcgggcgct
     3781 ccgtgtagag atgcgccagc tccggcggcg tctcccaacg gtcggtcagc atccagtcga
     3841 tgtcgggcat gccggtagta cagctctgca tgccgaccca cttgacttgc accggtgcca
     3901 ggcgctgcgc gcaggcagag atgcgcgagc cttcaccata gccgccaaga tcgatcagga
     3961 tatcgatgcc gcactccctc gccttgtgcg cgacggcctc gtcgttcagc gtggagatgt
     4021 cttcccattg ctgcgcgatc gcgcggaagc ggcgggcaag catgtcggac cacgtgttgt
     4081 gtgccagcgc gacgacggcg aacgcggcgg ggtccaaagc ttccagcccg gcaatcgtca
     4141 gccagcccac cggatgcgtc ttcagcaggc cagacaggag gccgatggtc agcttgcgtt
     4201 ctggctcccg cgcgttcgtg aacggtgtcg gcgtcgcgcg cggtatccgt tcggcgcagg
     4261 tccgggccgc atcggtgatc gcactgccag gtacgccgtc gctgtatacc agcgccgaca
     4321 gcaatgaacg acgcggattc aaatcgtccg gtgccaacgc gatagcacgg cgggcaaggg
     4381 cgaccgcttc ctcatgcagg ccgaggcagc acgtggtatt ctccagattg cacaatatgc
     4441 gcgcatccgg gccgtactcg tcgagcgccg attgcagcag cgtgcgcgcc tctccatgcc
     4501 ggtgcatgcg catcagcgtc gccgcgaggt cgtttcgcaa cgtggcgttc gtcggatcga
     4561 gctgtgcggc gcggcgcagg gccgcctcgc cttcgcgcaa gcgcaccgtg cggatcagtg
     4621 cctgtccgag aaatgccgcc ggaagtggcg cctcgggcgc gagcgcggcc gcgatctcga
     4681 atgactcgat cgcctctggc atccgcttga gatgttccag cagcacgcct ttggcgcagc
     4741 gcagcgcagc gttcagcgga tccgtgtcgc aattgaggtc cagacgagct agttcgtccg
     4801 cggccgcgcc cgccctgatc gcggcatcca cccggtgcaa cgcataattc agcgagtccg
     4861 gcgcgcggcg cagcgcttca cggaaggctg tgcgagccag cgcggggtct ccggtttgca
     4921 cgagcgaaat gcccagcgtg tcccaggcct cggcccggtc gggggcgatg ccgcagcagc
     4981 gcagcagcaa cggctgcgcg ctggcggccg caccccgcgc gatcagcaaa ccggccagag
     5041 cgaccagcgc ttcctcgcgg cgtgggttga tgtcgagcac ctgggtgtaa gcggcttctg
     5101 cgccggcggg atcgccatgg gcgcgcaggc tctcggccag gcgcagaggc ggttcgtccc
     5161 agtccggcag gcgctgcccc agctcccgca tcagtccgcg agcgcgcacc gggtcgcccg
     5221 cgcgatcctc ggcaatcgcc aggttcagca gagtggaggg cgatccttcg ccgagatctg
     5281 cggcttgccg caacgcgtcc agtgcgtcag ccgatctgcc agcggccagc aatgccatgc
     5341 caagcgccgc ctggtggggc gcctccccaa ggcgtggagc actccggagc aatgcaaggg
     5401 cgccggccga gtcgccgtta tccagacgtg ccagggcagc ttcagtgtgc attgccaagg
     5461 cccagcacga cgatcaggcc aagcactggt gcgtcggcct cacaccggag tatctccggc
     5521 gtcacgttgc ggacggccag gccggccgcc tcagcagcac gcaccagata ctgttcggcg
     5581 tgtgcgaagc gaccttggcg gccgagcgtc cagtcaccat tgccgggcat cgcgccttgc
     5641 gcgtcgggct gcagcagctc gaccgagaag gcgaacagcc ccgcaggcgt cagacgtcgc
     5701 tgaacggcga tgaacagctc atccagcgca ccgaagtagc acagcacgtc actggccaaa
     5761 atcagcggcc acgtgcgcgt atcggcgcgc aggaaatcga ggacatcggc ctcgtgcaga
     5821 tcggcgtaga gctgcttgcc ggcggcctgt gccagcatgc cgggcgcgat atcaacaccg
     5881 acgaacggcc cgaggtccag gtccgcgcac accacggcga gcaagccggt gccgcagccg
     5941 agatcgagca cggggccgcc atggacgccc tgcacctgca gcgcatcgtg cagcagccat
     6001 ggcacgcgat aaccgagcga cagcaaatgt gcctcgaagc gctcggcgta gccgttgaac
     6061 acagcacgga ggtagtcgat gggcgcgcgc gatgcgcccg gcaagacgcc ggccgcggcg
     6121 acgagatggc gcacatactg gtcttccggt ccaagcttga gcgcctcggt gtaggcgtcg
     6181 gccgcctcct cgtgacgacc gaggcttgag agggcgtgac ctttcaggcc gaacaggcag
     6241 gcatcgacca cgccggcgct gcgtgcctcc tcggcgatgg cggcggcggc ggcaaaatcg
     6301 cgccggcgga tgcaatgcag cacagctgcg ttgcgcaggc tcacatagcg aggggccgca
     6361 gcgatgccct cagtcaaggt ggcaagagcg gcatcggcgt cgcccgcgat atcggccgcc
     6421 gcagcgaatg cctcacggaa ggccgggttg ccaggctccg ccgccaccgc ctcgcgcagg
     6481 caggccacgg catcgtcggg ccggtgcatc tccagcagca gcacgccaag cagcgccttg
     6541 gcaaccggat cgtcccggtt cagcaccact gcctcggcgg cgtcggccgc agcgccaggg
     6601 atgtcgccca attcgcgacg caactccgcg cgcaacttgc gcaggcccgc atggtcgggg
     6661 gcgcgttcaa tggcaggatc aagttccgcg cgcgcgagct cgatttgccc ctcgcgcagg
     6721 gcgagccgga cggtcagctc gatctggccc ggcaagtccg gcgccatgcg gcgcaccgca
     6781 gccagcaacg cgcgggctgc accaacgtgg ccgtcatcca tcagcgacac ggcgcggcgc
     6841 agcaaaatgt caggttgcga attggctctt gcggtgtgca tcgcacgctc cttttgagct
     6901 gatgcaacgt ttcgacggac tcaaccgctg gccgacaggc ccgcggcgaa gtgctcgttg
     6961 atggagatca ggaagtcgaa attcggagcc ggccgatcca tctcacgctg caccgcaagg
     7021 ccgactgaaa cgatcgaagc ccgcaagtcg tcaggcagct cgttccttgg gtcacgcatc
     7081 aggtcggtga ctgccagcca gaggcgccgg ttgtcggcga tcgcgcgtgt ctgcgaaacc
     7141 ggttccccat cgcgcgccag cttgagggca ccgacggcac ggcggaacac gtcggcttcc
     7201 tgctcccgga cgctgcgaca gctggttgca gcctcatagg cacgggcccc ggttctcacg
     7261 ttcggcatgg ttctgcggtt tccctgtggg ttgcggatgc gtcatgcgtc atgacgcagg
     7321 ccggctgggc aacgcgaccc agcacggagt cctcgtggcg aacgatgcgc cgtgcgagtt
     7381 tcaatgcctg atagcagtca tcggcctcgg cggcggcgag cgcacgatcg aggatctcgc
     7441 aggccaatgc cgaagtggtg gcctccttga aggcgacgat cagctcacgt gccgcgacca
     7501 gtccgttcgc gcgctcctct tcggagccga tataggcggt ctgcaaggcg aaatagatgc
     7561 gccgcgccgg cgtgtcggcc tggtctggcg gcatgatctg cttgccaaac aggaaccgtg
     7621 ccttcgccgt gagttctatg cgcgacttcg ttcggaagcg gatgggtgcg ccattgacga
     7681 tcatcatctc gccctgacgc agctccagga ccaggttgga catgcgcccc ttcctagtgt
     7741 tcgtgcaaaa ccggagccag gccagatgtt cagtccggcc gggctctcgg ctgagcggtt
     7801 acttgaacag gctcaacagt gtctgaggcg cctggttggc gagcgacaga gcctgcgtgc
     7861 ccagctgctg ccggatttgc agggcctgca actgcgccga ttcctgtgcc aggtctgcgt
     7921 ccaccagtgc accgataccg gtgttgaggg cgtcgatttt gtcgctgttg tagctgacct
     7981 ggttgcttac gtacttctgc gccgagccga tcgtgttcag ctcgttgttc agcgcattga
     8041 acttattgat gaacgatgcc gtcgtcgcgg tcccgataat tccctgaacg gtggtcgctg
     8101 cattcagctg ggtgctggag tagctgatcg atccgaacag agccgaacca ctgtaagtcg
     8161 cgacagcata ggatgcgcca ctttcgttgc gcacgacggc gacgcgcgcg aatgtgccac
     8221 taccgccgct tatatcgccg accagtgttt tgccgttgta gttggcgtcc tggatgaacg
     8281 ttttcacgtt ggacagcagc gagttgtact gcgcaatata gttggtgcga tcagtgctcg
     8341 taacactgct gtcggacaac ttcaccaaca cgtcgcgcat gctggtcatg gtgttcgaga
     8401 tgttgttcag gctcgactga gtcgtggaca acaagccctg cacgttgcct agctgctggt
     8461 tcgcgctgcc cagcgcacca acggtcgagc ggacgttctg cgcgatggca taggctgcgc
     8521 cgtcgtcagt gctgtcggcc acacgaaagc cggtggagac ctgtttctgc gcggctgcga
     8581 gttgcgaatt cgtcttgttc agcgcttgga gcgcgatcat tgcgcccgtg ttggtgttga
     8641 cggagtttag gaaggacatg gtgtcttacc cttcaagtcc atgtgtgttg catacactcc
     8701 gccttttgcg ggtcttgcgg catcctgacg caccggagtt aacggccgat gaagttcacg
     8761 cttctttctc atccggtcgg gaggaatttc accagcgaca gcgcgcttac tccagcgatg
     8821 agctgataag aggcctgcat ttgcgtctgt acaagcgcga gctgcgatag cgtttgggcc
     8881 atatcgacat cctcgactga cgacacctgc gtggtcagcg ccgtctgcgt gtcggaaagc
     8941 tgggtctgtg tcgcattgat cgaagactga acattaccca gcacgccgga gtcttcattc
     9001 agcgcggtga ccgccccgtt caggctgatc cgcgtgtcct gcacaagatc cgcaaatccc
     9061 tgcacattcg cctggccgct gctgagcgag ccgatggtag ccaacgcgcg cagcacatct
     9121 cgcatatacg agccggtaga cgagacggga ccgccgacaa tcgtggtctg tcctggcggc
     9181 gacgtgggaa taagtccgtt ggcactcgcg ggtatgccgg tcgtctcctg gtgggtctgg
     9241 cccacttgca cgctcggcaa tgcagcctgc actgtcgcat acggctgcga gaggaaggtg
     9301 gaaaacggcg aggtgcctgc agcattggag accgcgacgg caaaggtggc gttcgcggta
     9361 tttgccgcgc cgttcaccgc gagaccattc acggcagcat tgatctgcgt gtaaaagccg
     9421 gagttcagga tattgtccga gtcgggcacc ggcggattgc cgctgtcctg gccggcgaac
     9481 acataggtac cgccatcctg agtatcgagc agtcccgcta cctgtttcag tgcgtcccgc
     9541 gcatttgccg ctatgctgtc gatctcgccg ggatcgactc cctgcaggtt gttgagctga
     9601 gcgtagaaat tggccgcgat cgactcgatc gagccgagcg acgactgcgc cacgctgatc
     9661 cggccagtcg ctgcgtccac attgttctgc caggtctgca ggttcgcgat ctgcggccgc
     9721 aggtcgagcg acgtgggcgc gccggtgcca aggccggcgt aggtggtgcc gatgaggccg
     9781 gtgctggcct ggatggtcag cttgtccagc ttctgcttga cggtgttgga ctgggcgatg
     9841 agtgtgttca tcatgccata gtccgatggg ccggtcacat tgctgatgac gccgctcatg
     9901 atcgcatcct catgacaccg atccaagcaa ctggttccac atggactgca cggccgcgat
     9961 caccttggca ttggcgccat aggcgttctg cagctggatc atgttggaca tttcggtatc
    10021 gatgcttacg ccgctctggg cagtgagctt gccgttcagc atggtctgca ccgattgttc
    10081 ggtggcgagc tggctggagg ctgtggtgct ctcctgtgcc tgtgcggcaa gcatcgtgga
    10141 agcaaaaccg cccagcgtcg gcggcgagct gaacggcgcg ttcagcgtac cagacgggcc
    10201 aatgccggtc acattggcgg caagcggcgg gttgccgcca agcgtgttgt tcagcaccgc
    10261 gttgatgatg gcagtgtagc cggcctgcgt cggcaccggc ggcatgtcgc catcgcgcac
    10321 catcgatgga ttcgccagca cagccggatt cacctggatc gtgccggcaa acccgacata
    10381 gccgttctgc gccggcggcg ccgccgcaac agggacagtg ccggtggggt ccgtgaacag
    10441 ggtcaggcca ttcgcctgga agcgcgtcgc catgctctcc gagagctcgt cgagctcacc
    10501 ctggtaggtc ggcagcgtcg tgtcgcgcaa agtgatgttg gcgccgatct ggccgccctg
    10561 gagctggcgc gtcacgtccg tgccgcccag caagatcgcc ggaatgccgc cgccgggata
    10621 gtatgtgccg ctctgcacat tcacatcact ggtgctgagc ggcggggcat tgccgtgtat
    10681 cggcaaggtc aggccagcgg tggtggtcag cagcacatcg ccgtttggct gctgcagcac
    10741 cttcacactg acaagctgtg agacggtcgc gatcgccgca tcacgctgac tctccaggtc
    10801 cgccgtgctc tggctgctcg ccttcagcgt gatgatctgg ttgctgaggc cgccgatggt
    10861 gccgagagtc gtgttcagcg tgccgatctc gttgacaata ttgtcctgcg cggtctgccg
    10921 ctgcgcgcca taggcgttgc tcagcgtatt gatgttctgc gcaagagtcg cggccgacgt
    10981 gactacctgg ctctgctgcg ccaggctgct tgggtcattc agtaaggtgg agaactggtt
    11041 ctgcaaattg gcaatcaggc tggcgatatc ggtgtcctga cctggcgtgc ccatcaccgc
    11101 atcgatcgac tgcaacgctg actgtcgcat ctgcaatcca gccaccgtgc cgttctggct
    11161 gaatacctgg gcctgcagtg ccagatcgac atcgcgtatg gcaggaccgg tatgcacgcc
    11221 caggccctgg ccgttggcta ccacatcgtg ctgtgtgccg atctcgcgcg catagccagg
    11281 tgtgctggca ttggcgacgt tctgcgacac caacgccaac tgggcgttga cgttgttcaa
    11341 cccaccggtc gcgatcgata atgcagacgc gatcgtcaca gctcacctac tgcttcatat
    11401 tgattgtctg ctgcagcatc tggtcggccg tcgtcaccat cttggtgttc gccgaatacg
    11461 cctgctgcgc gacaatcagt ttcgagaact cggtggcgat atcgacgttg gagccttcca
    11521 ccgattcggt gacgagagtg cccgcgccat tggtgccggg tgcttccgcg agcggagtgc
    11581 cggattcgct gct
//
LOCUS       DATGEM010000605         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1648948, whole genome shotgun sequence.
ACCESSION   DATGEM010000605 DATGEM010000000
VERSION     DATGEM010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648948"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1985
                     /locus_tag="VKQ27_18575"
     CDS             123..1985
                     /locus_tag="VKQ27_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY90995.1"
                     /translation="MIFDNCEHLTGAASDLIEALLTRVPGISVVATSQQALHLDAEQL
                     YLVQPLSVPPGDAVAIGGFAAVDLFVERANRADRQFRLGPANEAGVAEICRRLDGLPL
                     ALEMAAPRLRTLGVEGLREGLDHRLKLLKGAQHADDMRHRSLRAMVEWSHGLLDPQER
                     HLFRCLAVFPGSFTLEAAVAVDGGADRWTLVDALDRLVDKSLVTIEGGAPPRYRLLET
                     IRLFASERLQESGEGDAVSERHARHFVNTLDRAYVAWETTPDEDWIALYGPEIDNLRA
                     ALEWALAAPERRAIALALGASGLRLFDALSSVAEGLRYFERLVPLIDPDTPPVITAGL
                     LARAYYYFRDMPEPTLLTYLERAASLYRELDDRVNLGAALTLVAHSYARQGRFDQAKA
                     VLAEASELIERSNFPKLRMNLYQVIGISALQTGQIAEARTYFTQLLSITRALKSRYAA
                     RATTLLGLLECTAGDVDRAIEVGKEAVREARSVPGMILALALGNLASYLLARGDLREA
                     RGLLEEAFAHFVAAGSHNPAELQILAVLVGLEGRLADAARLIGFVDAERVRTAQVRQN
                     TEERLYGKLSRRLKAGLPTAEFAALKEEGASWAMPEAIEFVRSRLLSPGSGGGY"
     gene            2654..3574
                     /locus_tag="VKQ27_18580"
     CDS             2654..3574
                     /locus_tag="VKQ27_18580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLY90996.1"
                     /translation="MKIVVLGAGGVGGYFGGRLAAAGTDVTFVARGEHLNAIRRGGLR
                     IASPLGDILVSPAQAVESITKTGSADLVLIGVKLWDTEAVAPTLRPLVERGAAILSLQ
                     NGVQKDEVFRRHVPPEAVMGGVCYMPAVIEAPGVIRHNGTMQRLVFGEYGGARSSRAE
                     AFLDACSGAGIDGELSGDIEREIWDKFVVLIGISGTTSFFRQPIGPVRESADSRALLL
                     DAMREVVAVGRARGVHLSPEFAEDRLAFCDTLPATMTSSMHEDLKRGHRLELPWLSGS
                     VVEMGAAAGVATPANKRIADALAPFVQGRA"
     gene            3657..5354
                     /locus_tag="VKQ27_18585"
     CDS             3657..5354
                     /locus_tag="VKQ27_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLY90997.1"
                     /translation="MSTGRIIPLRSANARRGAAVLLEVLESEGVEYIFGNPGTTELPL
                     MDALLRAPALKYILALQEASAVAMADGYAQAARRPAFLNLHTAGGLGHGMGNLLNAAV
                     SRTPLVVTAGQQDSRHTVTDPLLFGDLVRIAEPAVKWAREVTAAEQLPVLVRRAFHDA
                     NAAPTGPVFLSFPMDVMEEMSSVDIAKQSVIDRGAIAGSLATLAKELASVRPGRLAII
                     AGDEIYWSQAAEETTALAEMLGASVYGSSWPSRIPFPTSHPLWAGNFPTKASAIAEVL
                     SNYDAIFALGGKSLITILYSEGSALPAGCELYQLSVDVQDLGRTYVTKLSVVGDIKAS
                     LRALLPLLQKETATRGQTYAELNTKAKESRAAQRGALVGLSDAQFNEPVVTPLVAARE
                     AVRAIGPKVAIVDEAIATSSLVRKFLNSESANQYSFMRGGGLGWGMPAAVGCSLGLGR
                     EPVVCLVGDGAALYSPQSLWTAAHEQLPVTFVVMNNREYNVLKNFMRSQVEYVSAQTN
                     QFIAMEINQPPIDYVALAQGLGIPARRIEKAGDIAPAIEAAIGSGRASLIDIAIGTS"
     gene            5648..6184
                     /locus_tag="VKQ27_18590"
     CDS             5648..6184
                     /locus_tag="VKQ27_18590"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY90998.1"
                     /translation="MSRIHTPALETATGATAEVYAQLKKAIGSVPNTFAAIGAHGPAA
                     LKAVLAADGVLATGSLSKQDQETIKLVVSEVAGCDYCVAAHFMLGKLTGLKPGTLNQV
                     RAGQPTDDAKRDALVRFVRTLVRTSGTISDAEFQAIRAAGYTDAQLVDITLAITVTVF
                     TNVFNRINNTDIDMPAVA"
     gene            6259..>6672
                     /locus_tag="VKQ27_18595"
     CDS             6259..>6672
                     /locus_tag="VKQ27_18595"
                     /inference="COORDINATES: protein motif:HMM:NF016139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HLY90999.1"
                     /translation="MNSLIKLAARSPLVTSDLDYHLMRAAMVLIFALFGYQKWFDYEA
                     QVLIPFISNGPFIFWLEPLLGVHGASYFLGFSEWLFGALLFAGFWNKPLGILGAIGST
                     GTFVMTVTIIPFMPNGWAAVGFPAMAINNAFLLKDV"
BASE COUNT         1181 a         2152 c         2086 g         1253 t
ORIGIN      
        1 cgccgctgtc cgagccggca caggtcccga cggcagtcgc aaccgtcctt cgcctggacg
       61 cgtcgactat ggaggcggca gtgcgcgcca tcgctgcgac gctcttaagg cgccggagcc
      121 tgatgatctt cgacaactgc gagcacctca cgggcgccgc ttccgacctg atcgaggcgc
      181 tgttgacacg ggttccgggc atctcggtcg ttgcgaccag ccagcaggcg cttcacctcg
      241 atgccgaaca gctctatctc gtgcagccgc tgtccgtgcc tcccggggac gccgtcgcga
      301 tcggcgggtt cgctgcggtc gatctgttcg tcgagcgcgc caaccgggcc gatcgtcagt
      361 tccggctggg cccggccaac gaagcgggcg ttgccgagat ctgccgccgg ctcgacggct
      421 tgccgctggc gctcgagatg gccgcgccgc gcctgcgcac gctgggcgtc gagggcctgc
      481 gcgagggcct cgatcaccgc ctgaagctgt tgaaaggcgc gcagcacgcc gacgacatgc
      541 gccaccgctc gctgcgggcc atggtggagt ggagccatgg gctgctcgac ccgcaagagc
      601 ggcatctgtt ccgttgcctc gctgtgttcc ccggcagctt cacgctcgag gcggcagtag
      661 cggtcgatgg cggcgccgat cgctggacgc tcgtggatgc gctcgatcgg ctcgtcgaca
      721 agtccctggt cacgatcgag ggcggcgccc cgccgcgcta ccgcctgctg gagacaatcc
      781 ggctcttcgc cagcgagagg ctgcaggaaa gcggcgaagg cgacgccgtg tcggaacgcc
      841 acgcgcgcca cttcgtcaac accctcgatc gcgcctacgt cgcctgggaa acgacaccgg
      901 atgaggactg gatcgcactc tatgggccgg agatcgacaa tttgcgggca gcgctggagt
      961 gggcgctcgc cgcgcccgag cggcgcgcca tcgccctggc gctcggggcg tccggcctcc
     1021 gccttttcga cgcgttgtca tcggtcgccg aaggtctgcg gtacttcgaa aggctcgtcc
     1081 cgctgatcga cccggacacg ccgccggtca tcaccgccgg gctgctggcg cgcgcctact
     1141 actactttcg ggacatgccc gagccgacgt tgctgaccta tctcgagcgg gcagcttcgc
     1201 tctatcgcga gctcgacgat cgggtaaacc tgggcgccgc gctgacgctc gtcgctcact
     1261 cctatgcgag gcagggccgc ttcgaccagg ccaaagcagt tctcgctgag gcgtccgagc
     1321 tgatcgagcg cagcaatttc ccgaagttgc ggatgaatct ctaccaagtc atcggcataa
     1381 gcgcgctgca gacgggacag atcgccgagg ctcgaacata tttcactcaa ctactcagca
     1441 tcacccgtgc gctgaaatct cgttacgccg cgcgggccac caccttgctc gggctgctcg
     1501 aatgtaccgc cggcgacgtc gaccgggcga tcgaggtcgg caaggaggcg gtacgcgagg
     1561 cgagatcggt cccaggcatg attctcgccc ttgcactggg caacctcgcg tcttacctgc
     1621 tcgctcgcgg ggatttaagg gaagccagag gcttgctcga agaagcgttc gcccacttcg
     1681 tcgcggccgg ctcccacaac ccggccgagt tgcagatatt ggcggtgctc gtcggcctcg
     1741 aagggagatt ggctgatgcg gcacggctca tcggcttcgt cgatgcggag cgcgtgcgaa
     1801 cggcacaggt gcgccagaat acggaggagc gactctacgg caagctctcg cggcggctaa
     1861 aggcgggact gccgacggcc gagttcgctg ccctgaagga agaaggcgca agctgggcaa
     1921 tgcccgaggc catcgagttc gtcaggagcc ggctgctatc cccgggatcc ggtggtgggt
     1981 attgattatt agagcccgtc gggcgccagc gtaatcgtgg cggtggaagg cacaggcgca
     2041 ggcgcagctt gcggccgcct cccgcttgac ggcggtctcg cctcagcgtc gtagcccctg
     2101 aaggtccgca tgggcgacca ctccggcatc ggcggcgacg ggggattctc gtcgaacgac
     2161 gcgaaagcgc cctcactcca gacgacgcgg ccgcccacga tcgtgagatc ggatgtgatg
     2221 tccgctattt cgctctcgca gcaatcgaaa cggtcccggt caggcacgat caagccggcg
     2281 aactgtgctg cggcgatccg gcccttcctt ccctcctcgt cagagagcca tgtcgtcttc
     2341 tccgtccaca ttgtgaccga catcccgatc ccacgccaag ggccagtgat agctttgctc
     2401 ggtcgctgct cacaggcgaa ttaaggcgac gcgaatcggt gcaaacgcga tcggggccgg
     2461 ctacgcacct ttaagcatca cttaactaga cgtgagagcc cgcggcggtg caagtaaagg
     2521 cctgcctgat atttgcgagt gatgattagt ctcataagtc cgatttgccg aatatgatga
     2581 gcgcctctct gcgactgagg cgttttttca cacttcgctt gcgacgacat tcgaagtgca
     2641 gtcggaggaa gttatgaaga tcgtggtgct cggagccggc ggcgttggag gatatttcgg
     2701 aggccgtctg gcggcagccg gtaccgatgt gacgttcgtg gcacgtggcg agcatctgaa
     2761 cgctattcgt cggggtggtt tacgcattgc gagcccactg ggcgacatcc ttgtttcccc
     2821 tgcgcaggcg gttgaatcga ttacgaagac tggttctgcc gatctcgtcc tcatcggagt
     2881 caagctctgg gacacggaag cggtcgcgcc taccctccga cctttggtgg aacgcggcgc
     2941 agccatcctt tccctgcaaa atggcgtgca aaaggacgag gtattccgtc ggcatgtgcc
     3001 cccggaggcg gtgatgggcg gcgtgtgcta catgcctgcc gtcatcgagg cgcccggcgt
     3061 catccgtcac aatgggacaa tgcagaggtt ggtgttcggt gagtacggcg gtgcgcggtc
     3121 ctctcgggcg gaggcgtttc tcgacgcttg cagcggtgct ggaatcgacg gcgagttgag
     3181 cggtgatatc gaacgcgaga tctgggataa attcgtcgtt ctgatcggaa tctcaggaac
     3241 aacctccttc ttccgccaac caatcggccc ggtccgcgaa agtgccgact cccgtgcgct
     3301 gctcctggat gccatgcgag aggttgtcgc cgttggacgg gcgaggggcg tgcatctgtc
     3361 acctgaattc gccgaggatc ggctcgcgtt ctgcgacacg ctcccggcca caatgacctc
     3421 atcgatgcat gaagatctca agcgaggaca tcgccttgaa cttccttggc tgagcggcag
     3481 cgtggtcgag atgggcgccg cggccggcgt tgcaacgccg gccaacaaaa ggatagcgga
     3541 cgcgttggct ccttttgtcc aaggaagggc gtgatcgagg gcgagacccg attgtcggct
     3601 ccgctcataa agccgcagaa atcaccgtcc ccgtgtcatc tgggaaggag gcaacgatgt
     3661 cgactggtcg aatcatcccg ctcaggagcg cgaatgcccg acgtggcgcc gcggtgcttc
     3721 tcgaagttct ggagagcgaa ggggtcgaat atattttcgg caatccgggg acgacagagc
     3781 tacctttgat ggacgctctg ctgcgcgcgc ctgccctcaa atacatcctc gcactccagg
     3841 aagcgtccgc cgtcgcgatg gctgacggat atgcgcaagc cgctcggcgg ccggcatttc
     3901 tgaatctcca taccgccggt ggccttggtc atgggatggg aaatcttctg aacgccgccg
     3961 tgtcgcgcac cccactggtt gtcacggcag ggcagcaaga ttcccgtcac accgtgacgg
     4021 atccgttact tttcggcgac cttgttcgga tcgccgagcc ggcggtgaaa tgggcccgcg
     4081 aagtcaccgc ggccgaacag ctacccgttc tggtccgacg agcgtttcac gacgcaaatg
     4141 cagcgccgac tggtccggtc ttcctgtcgt tcccaatgga cgtcatggag gagatgagtt
     4201 ccgtcgacat tgcgaagcaa tcggtcatcg accgtggtgc cattgccggg tcccttgcga
     4261 cgctggccaa ggaactggcc tcggtccgtc cgggccgatt ggcgatcatc gctggagatg
     4321 agatctactg gagtcaggct gctgaagaga cgactgctct agcggagatg ctcggggcgt
     4381 ccgtatacgg atcgtcgtgg ccttctcgga taccgttccc gacttcacat ccgctatggg
     4441 ccggcaactt tccgaccaag gccagcgcaa ttgccgaagt cctctcgaac tacgacgcca
     4501 ttttcgcgct cggcggcaag tcgctgatca cgatcctcta ctcggaggga tcggcattgc
     4561 ctgccggctg tgagctgtac cagctttcgg tcgacgtgca ggaccttggg cggacgtatg
     4621 taacgaagct ttcggtcgtc ggggacatca aggcctcgct tcgcgccctc ctgcctctcc
     4681 ttcagaagga aaccgccacg cgaggtcaga catatgcaga gttgaacacg aaagccaagg
     4741 agagccgcgc cgcgcagcgc ggcgcgctcg tcggcctttc agatgcgcag ttcaacgagc
     4801 ctgtcgtgac accgctcgtc gccgcccggg aagcggttcg tgcaatcggg cctaaagtcg
     4861 cgatcgtcga cgaggccatt gccacctcat cgctcgtccg aaaatttctt aatagcgagt
     4921 ccgccaatca gtactctttc atgcgggggg gcggcctcgg ttgggggatg ccggcagccg
     4981 tcggctgttc gctcggtctc ggacgggaac ccgtggtgtg tctggtggga gacggcgccg
     5041 cgctctattc accgcagtcc ttgtggactg ccgcacacga gcagctgccg gtcacgtttg
     5101 tggtgatgaa caaccgggaa tacaacgtgc tgaagaactt catgcgatcg caggtcgagt
     5161 atgtgtccgc gcagacgaac cagttcatcg cgatggagat caaccaaccg cctatcgact
     5221 atgtcgcact ggcgcaaggt ctgggcattc cggcgcgacg catcgagaag gctggcgata
     5281 tcgctcccgc catcgaagcc gccatcggga gcgggcgcgc cagcctgatt gacatcgcta
     5341 tcggcacatc gtgagtcctg acgcgtcatt ttgggacaag gctcgccagc gagttcccat
     5401 gcagcggttt agctgcgcac cgataccgca cttcttagta tcgcttagct cgacgcgaga
     5461 gcctcgaggc tgcgcgtaga gagccgtatt gggtctgcgg gcaacgattg gttctacaga
     5521 tcttatgcgc cggatatagc ggcgcaactc tctgagctgc ggccatcttg gcccaaagca
     5581 gtcacgatca ggtcgatctc tgacaccaaa gaaaatcgaa gatcataatc gaggaggagc
     5641 acaaaccatg tcccgcattc atacccctgc cctcgaaact gcgacgggcg ccaccgccga
     5701 ggtctatgcc cagctgaaga aggcgatcgg cagcgtcccc aacaccttcg ccgccatcgg
     5761 cgcgcatggc cccgcggcgc taaaggccgt gctcgctgcc gacggcgtgc tggccaccgg
     5821 tagcctctcg aagcaggacc aggagacgat caagctggtc gtgagcgagg tcgccggctg
     5881 cgattactgc gtcgccgccc acttcatgct cggcaagctc acgggcctca agcccggcac
     5941 actcaaccag gtccgcgccg gccaacctac ggacgatgcc aagcgtgacg ccctcgtccg
     6001 cttcgtccgc acgctcgtcc gcacaagcgg cacgatcagc gacgccgaat tccaggcgat
     6061 ccgggccgcc ggctacaccg acgcgcagct ggtcgatatc acgctcgcca tcaccgtcac
     6121 cgtcttcacc aacgtcttca accgcatcaa caacaccgac atcgacatgc ccgcggtcgc
     6181 ctgaccgccg gccagccggc gagcgcgacc gccccaggcg cgtccgcccc tctcggtgtc
     6241 ttccgaggag ccacagccat gaattcgctc ataaagctcg ccgcccgctc gccgctcgtc
     6301 acgagcgacc tcgactatca cctgatgcgc gccgccatgg tgctcatctt cgcgctgttc
     6361 ggctatcaga agtggttcga ctacgaggcc caggtgctga tccccttcat cagcaacggc
     6421 ccgttcatct tctggctcga accgctgctt ggcgtccatg gcgccagcta cttcctggga
     6481 ttctccgaat ggctgttcgg cgcgctgctc ttcgccggct tctggaacaa gccgctcggc
     6541 atcctcggcg ccatcggctc gaccggcacc ttcgtcatga ccgtcaccat catcccgttc
     6601 atgccgaacg gctgggccgc ggtcggcttc cccgccatgg ccatcaacaa tgccttcctg
     6661 ctgaaggacg tc
//
LOCUS       DATGEM010000606        23617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_508711, whole genome shotgun sequence.
ACCESSION   DATGEM010000606 DATGEM010000000
VERSION     DATGEM010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 23617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23617
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508711"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            933..1067
                     /locus_tag="VKQ27_18600"
     CDS             933..1067
                     /locus_tag="VKQ27_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91000.1"
                     /translation="MGLTNTELLYYDANVLRLPADKRSEYHGQVDRLVAALRSALADK
                     "
     gene            1077..1880
                     /locus_tag="VKQ27_18605"
     CDS             1077..1880
                     /locus_tag="VKQ27_18605"
                     /inference="COORDINATES: protein motif:HMM:NF041116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HLY91001.1"
                     /translation="MTRVMKAGSFAKHTILRKTANDPVDVDVVFYIAEPDANHDTLQE
                     LNDAIFELLVAQYPTKAVEDFEIQRRTAKVVFVKSGLTVEVVPVIENPDRAGYGWQFD
                     LETGAKVETYPAGQIKFVRDRKSVDPHFRTLVRMGKRWRNHAELTFFKSFQIELIMAY
                     VLDTQGSDGSIDQRFRNFLLYIAQSGLKETISFPENSQPIASFTGPVVIVDPVCSWNN
                     VAARLTEEERLEIIAAATEAWETANFATVEDDHDLWKELFGPRFKTEAA"
     gene            1880..2377
                     /locus_tag="VKQ27_18610"
     CDS             1880..2377
                     /locus_tag="VKQ27_18610"
                     /inference="COORDINATES: protein motif:HMM:NF037203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91002.1"
                     /translation="MSISASRSASETYSDIDVGNVVRRVGADLRMIADSTGCWTPVMT
                     ANYVHDIEELAKGGYLKFVDVTLISNGVEIRATRFSVITNAGDAANQRPGDALWPKIP
                     GATLRIILSHTPEYNQAAEKAMQLKLRANWVSSSADTSHGGLTQNGGRSYASNSFGMP
                     RTDWK"
     gene            2374..3267
                     /locus_tag="VKQ27_18615"
     CDS             2374..3267
                     /locus_tag="VKQ27_18615"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY91003.1"
                     /translation="MSDNKVFDSEIPLPDDRLSAREPMLLGFDARYARVHDQLRLLLQ
                     ADELAAWNKKHHGGRLALASLATDQYPLVIFHGDVGTGKTVTAECIANRLVSEAGADD
                     SILFKLSNRVRGSGLVGEMGTQLVEAFRKIEQSAGKHRRAILVIDEGDSLGASRSQAH
                     SHHEDKVAVNTLIQEIDQLRRLGGRIVVFLCTNRLSVLDPALRRRAAVIEEFRRPTES
                     ERHELLSKDLAALNLSKDEIARLVAVSGEREGDPPWTFSDIRTRFYPAALAMAFPCSP
                     LTFAHLEAAATAMRASPRLED"
     gene            3270..4433
                     /locus_tag="VKQ27_18620"
     CDS             3270..4433
                     /locus_tag="VKQ27_18620"
                     /inference="COORDINATES: protein motif:HMM:NF036729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91004.1"
                     /translation="MPSSSLFGRRIHIAGSISGDPGGASQDQIAYARLLLQRLVPELV
                     RRGANFVIPVDREPVRGDGEKICFDWLVWEGVHAAFTTRPPEAPGPFIIAVQHHKSED
                     QVPAEQWELWEALRASPAVQIESAAQWNMASKRMEVAARTGDILIALGGAEGVDYLAN
                     LYHDAGKPVVPLDLPITGPNAGARKLFAFGQSPKNAARLFRSKGDMPPHSWLSRIQHT
                     RRQTADALASGLTDLLEALAPPRAFGVRLLNPNHPHFDAVEAHFETVVRPVIEDELGY
                     ELTVIDGSQPFEHSRIDQEIFTKLHRSRVVLADITGSRPNCFIELGYALGRQLPTMVM
                     GLEGAELPFDITTISGLMWKAAGPVKERRTAFRNHWIAIKNRPPIVPVEPLIP"
     gene            4430..4987
                     /locus_tag="VKQ27_18625"
     CDS             4430..4987
                     /locus_tag="VKQ27_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HLY91005.1"
                     /translation="MTKRQEAFVSVDVETSGPIPGEFSMLTIGACLIDDPTKTFSCSL
                     KPISLKADPAAMAVTGLSLDQLSDAGLPPEVAMRRFADWLRSLAPDGDIVFVGMNAPF
                     DWSFVNYYFWRFYGQNPFGFTALDIKAFYMGMTGCRWDETRSSKMADRLSPTSKGTHD
                     ALQDAIYQAELFRLARQLAVPDGEA"
     gene            5683..6168
                     /locus_tag="VKQ27_18630"
     CDS             5683..6168
                     /locus_tag="VKQ27_18630"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HLY91006.1"
                     /translation="MEGLLAQSAPSVPVPVENMVTERGIELRYGDLEDVSGLLVRDAD
                     GVTIGVNSTHSPVRQRFTIAHEFGHFLLHEGILEHVDRGYRVNYRSALSSEATDVEEI
                     EANFFAASILMPRSYLDARSAVLAVDDDTQVKALAQEFHVSQHAMSLRLANLYRQYAP
                     Y"
     gene            complement(6492..7097)
                     /locus_tag="VKQ27_18635"
     CDS             complement(6492..7097)
                     /locus_tag="VKQ27_18635"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91007.1"
                     /translation="MPGLISRFTGEHAGILFRTVEAHQDHLIRGLHDGSLDLALTYSL
                     DLTEEIIAHYMRDLIGHAPYAQSQQVEFRAWLKQFAHWLFDTDHISVRYEIVYDGIDI
                     RKLSPGTRGIVLLLLYLALDDGDDRPLIIDQPEENLDPKSVFEELVALFVAAKAKRQV
                     IMVAESSRVTFRHGFDCRRTDDVVARLRRWERPFSGGDRCR"
     gene            complement(7097..7282)
                     /locus_tag="VKQ27_18640"
     CDS             complement(7097..7282)
                     /locus_tag="VKQ27_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91008.1"
                     /translation="MQLFIRHHASGLSLTQAGRDLLGQARNLLKIAEELQMAAKEMDG
                     GMTGTIALGFLVRWRRR"
     gene            7563..8834
                     /locus_tag="VKQ27_18645"
     CDS             7563..8834
                     /locus_tag="VKQ27_18645"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLY91009.1"
                     /translation="MTVETTSIDTLVVGAGQAGVAMSEHLRELGVPHLVLERDRIAER
                     WRTGRWDSLVANGPAWHDRFPNLEFPGVSPDGFASKDEVAEYFVAYARKLAGPIRTGV
                     AVTKVVRNADRPGFIIETSDGTIEANRVVVATGPFQRPLIPPIAPKAEALMQIHSANY
                     RNPGQLPDGAVLVVGAGSSGVQIADELQRAGRQVYLSVGAHDRPPRAYRGLDYCWWLG
                     VLGEWDTEVKKAGREHVAIAVSGARGGHTVDFRRLAEQGITLVGLTKSFDHGVALFEA
                     DLATNIANGDENYLAVLDAADAYAVRNGLDLPEEPEARIIPADPHCLTHPLLELDLAK
                     AGVTSIIWATGYATDFSWLQVDAFDETGKPKHQRGISKEPGVYFLGLPWLSRRGSAFI
                     WGVWHDARHIADHIGIQRRYLAYYEARTTPR"
     gene            8846..9277
                     /locus_tag="VKQ27_18650"
     CDS             8846..9277
                     /locus_tag="VKQ27_18650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY91010.1"
                     /translation="MMSQPTHTRIRMFNTQDTYPNQRLDNDLCQAVRAGNTVYVRGQV
                     GTDFDGNLVGLGDPRAQAEQAMKNVKQLLAEAGSDLTHIVKTTTYLTDPRYREPVYQE
                     VGKWLKGVFPVSTGLVVSALGQPQWLMEIDVIAVIPDGWTS"
     gene            9274..9951
                     /locus_tag="VKQ27_18655"
     CDS             9274..9951
                     /locus_tag="VKQ27_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HLY91011.1"
                     /translation="MTFSIVARCPRSGRFAVAVASSSPAVAARCAHARAGVGAVTTQN
                     VTDPRLGPQMLELMQLGASAREAVDIVRRSAPHIEHRQLTAVDKDGATAGFSGERALG
                     CHASAEGRNAVAAGNLLADPGVPQVMVEAFQAGAAEGDLGDLVIAAMQAGLAAGGEAG
                     PIRSAGFLLVDRVAWHVADLRVDWHEEPLAELARLWQLWRPQMQAYVDRALDPAAAPA
                     YGVPGDE"
     gene            complement(10070..11041)
                     /gene="speB"
                     /locus_tag="VKQ27_18660"
     CDS             complement(10070..11041)
                     /gene="speB"
                     /locus_tag="VKQ27_18660"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLY91012.1"
                     /translation="MTGEPNPPEFSDRMVQPRYTGIPTFMRAPFVEDPGALDIAMVGV
                     PYDGGVTNRPGARHGPREVRNQSSLMRRINQATGIDPYALCRVGDIGDAWVAKPYELE
                     GAHREIEAFYAGLHRASAVPLSVGGDHSITLPIFRAIAKERPIGMIHFDAHCDTGDDY
                     LGSRFHHGAPFRRAVEEGLLDPKRVIQIGIRGGINDRDIWRFSHESGMRVVYMEEFYD
                     RGPKSVIEEARSIVGDGPTYVSFDVDGLDPAYAPGTGTPECGGMSVLEAQIMVRRLRG
                     LNLVGGDVVEVAPPFDPSGNTALVGASMMYEILCVLAEAVAARKAHA"
     gene            complement(11079..12044)
                     /gene="speB"
                     /locus_tag="VKQ27_18665"
     CDS             complement(11079..12044)
                     /gene="speB"
                     /locus_tag="VKQ27_18665"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLY91013.1"
                     /translation="MAQNEYDSGRLNLPFVGHCSFAKRPICLDWDRIDGHGAILGAPF
                     DMGTQYRAGARFGPRAIREASTLFSFGHGGAYDFEDDVTYLPVDKVRIVDIGDADIIH
                     TDTMRSHANIELGVRKILAAGALPIVLGGDHSVHIPCIRAFADQPPVHIVHFDAHLDF
                     VDDRHGVRFGHGNPLRRASEMAHVTGLTQLGIRNVSSSNRNDYAAARAAGSTILSVRD
                     VRRLGKEQVLARIPRGARYYLTIDIDGFDPSIAPGTGTPSHGGFLYYEVLEILQGLTG
                     QGDVVGIDLVEVAPDYDHAGTTAFLAAQLLMNLLGFIFHAKGLSN"
     gene            12505..13491
                     /locus_tag="VKQ27_18670"
     CDS             12505..13491
                     /locus_tag="VKQ27_18670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY91014.1"
                     /translation="MRRFFGLLLAATAAVVSCGALSGPAAAADTVKIAHSTWVGYGPL
                     YIARDKGIFKKNGVDVDLVVLEDPKERFPALMADQIQMIASTVDTALLYLKRPTDFQY
                     VVGIDDSKGGDGIVAIKDIKTVADLKGHTVGVSEGSVSEFYLNVLLGQAGLKESDLKT
                     VNMTAADAGGAFVGKRIDAAVTWEPWLSRGKATDFGHLLVDSSTTPGLITDAVIVKTD
                     WMKAHEKDVAAIVKSWNEAVAYYREHPDESIEIMAKGVGGWLKDPKDFKSTLPGIKFY
                     GAEDNKVFFGTKSKPGPLYKTVESAIDIWSSHGKVQVKVTPDDLINYSFVNE"
     gene            13491..14345
                     /locus_tag="VKQ27_18675"
     CDS             13491..14345
                     /locus_tag="VKQ27_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91015.1"
                     /translation="MAAFEEYTAPKTETPRQRLMAAFEEYTAPKTEIPRRVYLTVSTI
                     VAAALLLLWCVLAYGKFVREDFLPTPVAVVAAAAAGIRDNSLIVNTAVSVSEILSGFV
                     LASIIAVPLGILMGSFKIVEAAIEPVTNFMRYLPVSALIPLLILWIGIGIEEKIAVIF
                     LGTLFQQLILIADVSRLVSADLLDVSYTLGANRRVVIRRVLIPATMPGVMDTLRVTLG
                     WAWTYLVVAELVAANKGLGYFILNSMRGLFTDQIFLGIIVIGLLGLATDQLFKALRSW
                     MLPWATTA"
     gene            14315..15142
                     /locus_tag="VKQ27_18680"
     CDS             14315..15142
                     /locus_tag="VKQ27_18680"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91016.1"
                     /translation="MDASLGNHSLSLPGRSPAAKLRIDKVTLRFTPRSGNPVTALDGI
                     SLDVADQEFSVIVGPSGCGKSSLLRLVAGLARPTAGAIHLDGKAVTKPGKDRGMVFQS
                     YTLFPWLTVRQNVEFGLEVAGLDAATRARIAGRFISQVGLDAFVDAYPKQLSGGMMQR
                     VALARALANDPEILLMDEPFGALDSQTRSLMQELLLNIWESSHKTVLFITHDIDEAIL
                     LGDRVYVMTARPGRIKEVIEIDIPRPRTVEVLTSAPFMQLKRRIMALIHEEAVRAMG"
     gene            15523..16881
                     /locus_tag="VKQ27_18685"
     CDS             15523..16881
                     /locus_tag="VKQ27_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HLY91017.1"
                     /translation="MRGRLMEGRSMDVDWQADLERWLAPFVAALRHKTRARMCPLYVA
                     GLIGPGDRKSVQPMAARAGEVGYDQLHHFVAAGVWNSAPLEAALLKEADGLVGDEAGF
                     LVIDDTALPKKGRHSVGVAPQYASSLGKTANCQSLVSVTLASREVPVMVGLRLFLPES
                     WTDDPERMARARVPKHRQTALTKPEIAIEEIDRVIASGARFGCVLADAGYGSSGPFRQ
                     ALSERGLLWAVGLSRRQNVYPADVGLIFPVAKAGKPRKHHIPDQPPVSAEALLAAAKW
                     QKVSWRRGTKGRLTCLFAARRVRVADGHKHRMLDNRMQCMPGDEVWLVGERRSTGEQK
                     YYVSNLPADAAIKTLAAAIKARWVCEQAHQQLKEELGLDHFEGRSWTGLHRHALMTMI
                     AYAFLQSRRLKAVGRKKKSLGTATTTKHAGHQASDPRPIRATAIPMMSALWQPPGGCR
                     ET"
     gene            complement(17231..18268)
                     /locus_tag="VKQ27_18690"
     CDS             complement(17231..18268)
                     /locus_tag="VKQ27_18690"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HLY91018.1"
                     /translation="MNTNSRRANRLAVAIFGAAGHTGRFVVAELQRRGIVPIAIARDT
                     AALTANFPGDEIQRRQASADDATSLDRALDGAQAVINCAGPFLDTADAVVSAALRAGI
                     HYLDVTAEQPSARATLDSYDAAARHAGIALIPAMGFYGGFADLLVTAALGDWDNADTI
                     EIMIGLDSWHPTRGTRITGERNTARRVVISEGRLVPVALPAAEKLWEFGDPLGRQAVV
                     EVPFSEIILIARHVKTAELHTYLSSNALSDIRDSATPAPEPADATGRSPQRFVVDVVV
                     KHAGESRRVVARGRDIYAFSAPLVCEAAERLLKGEFSSGGTHAPGAILDAKDVLAALA
                     PDHLTFEMKVA"
     gene            18375..18971
                     /locus_tag="VKQ27_18695"
     CDS             18375..18971
                     /locus_tag="VKQ27_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY91019.1"
                     /translation="MPKTAAISDAHSTAEVRARILETACMLFYQRGVRAVGVDLVVEK
                     AGVAKTSLYRHFGTKDDLVAAFLEREDEDFWGTWDRVANRHSDDAAGELDAHLEWIGE
                     RVGRTNYRGCPQINVAAEFPEIDHPARKVAAAHKRQMRQRLKRIAERLGVARPDELAG
                     QLSLLINGAFVSSQILELGEPTPLLRSAAHALIEASRT"
     gene            19178..19852
                     /locus_tag="VKQ27_18700"
     CDS             19178..19852
                     /locus_tag="VKQ27_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HLY91020.1"
                     /translation="MTLQAKLDAFKADFEAGKPPYSVPRSVIAIMHRATKELIASGAA
                     ERALKVGDKAPAFTLKDPEGNEVSSLALLQNGPLVVSFYRGVWCPYCNMELQALEAAA
                     PAFRRLGASLVAISPQTAPNSRKSVRQNSLSFPILSDTHNDVAAAFGLRFALPDYLAE
                     LYKNRKNDLPNFNGDDSWTLPMPGRFVIGQDGTVLYAEVNPDYTRRPEPEDMIPALER
                     ASIVHA"
     gene            19908..20627
                     /locus_tag="VKQ27_18705"
     CDS             19908..20627
                     /locus_tag="VKQ27_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91021.1"
                     /translation="MTRRSFLITGASKGIGRALSARLTAAGHQVVGLARNADASFPGV
                     LVPVELGDRAATAAVLADLVQRFEFDGVVNNVGLVRPQRLGDIDLDDLDAVLRVNLHS
                     AIQTAQALLPQMKAKGWGRIVNISSLTILGIVERTAYAAAKAGLVSFARSWALELAQT
                     GITVNSVAPGPTETELFRANSPPGSEGERRYLSVVPMGRFGKPDEIAAAIAFLLSEEA
                     GFITGQTLFVDGGASIGKASI"
     gene            20909..21820
                     /gene="gcvA"
                     /locus_tag="VKQ27_18710"
     CDS             20909..21820
                     /gene="gcvA"
                     /locus_tag="VKQ27_18710"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HLY91022.1"
                     /translation="MSRRLPPLAALRAFEAAGRLLSFSKAGDELLVSQSAVSHQIRQL
                     EASLGVPLFRREVRRVSLTPEGATYFATVRRAFDLLAEGTEAVAAPTKATVRVSLLAS
                     FATHWLVPRLGRFRARHPGVALALEPSIALVDLETGGADLAIRYGRGGWPGVNARKLM
                     DEAVSPVASPALMARGPADAPGDLLGHELLFSYSPRLFEWESWSRAAGLDLGTARRSL
                     LHDYNIVLQAALDGQGIAMGRRRLIRDELAAGRLVQPFALQIRGDIAWWLVLPRRKLG
                     PAAQAFVDWLDEEAATDEFDSSIAVTL"
     gene            21887..22357
                     /locus_tag="VKQ27_18715"
     CDS             21887..22357
                     /locus_tag="VKQ27_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY91023.1"
                     /translation="MIDTIAARLERLGHRLPEVSGPAANYVPVTRVGALAFISGQVAP
                     FAPGDEPGARLGAELSVEAGQKAAAAAALSLLAQIDQLVAGNPAGVRRVARLGVFVAA
                     TLEFNQHSLVGNGASDLVVAVLGEAGRHARTAVGVASLPRGAAVEVEAILELAE"
     gene            22363..23526
                     /locus_tag="VKQ27_18720"
     CDS             22363..23526
                     /locus_tag="VKQ27_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLY91024.1"
                     /translation="MGLDIAKLRTETPGADRVLHLNHAGSSLMAAGTVAAIEDQLRRE
                     MEAGAMEAGAAVAARIDQVRADIATLVGAAADEIAFTGGNSDGWGRAFAALRLKPGDR
                     VLVGRSEWGGNLACLTRAGLAIETVPADDHGQISLDALERMLDGRVRLLALTWAPANG
                     GLIQPAAAVGRLARAAEVPYFLDAAQIVGQWPVDVAELGCDVLTAPARKHLRGPRGVG
                     FVYVRSGFAARLEPTFVDTQSAPIGPDGAVLKPHARRLESAEGSAALRLGFGAAVRQT
                     LELGVDRIRAAVAGRAESLRSRLAAMPGVQLRDLGIEGSGLVSFTLDGRSGADVKKAM
                     AAQGINLGLNGRAFTPLDMDARGLTEVLRASVSYLTTEAELDRFQAALAALVG"
BASE COUNT         4203 a         7603 c         7635 g         4176 t
ORIGIN      
        1 gaattggcgc tcgacggctc gctgaccccg gtcgccggcg cgctgcccgc ggccatgggg
       61 gccgccgagc ggcagcgcgg gctgatctgc ccggccggct gcggcggcga ggcggcctgg
      121 gccggtgatg cgagataaaa tagtcctttc cactgaggcc gccaaaaacg gcggaaagcc
      181 gggaagtctc ccagtccgtg cttcgtatcg atgataataa aaatgtttcc acgttaaccg
      241 ttccgttaac caatcgaatc acttgagcgt gccgtggaat gacggaagtg ggcgctctgc
      301 cgccaggggg ctcttcggct tgagtgggct tgcagcggaa tggccgcttc cgagccgtat
      361 caaatgaaag cagcccttct ccgacctcga gccgccggca aaattcgtcc catggcagtc
      421 gccgaccact catccatgta gtccacagtg ggaatcaatg cgtgacccga cgccccgacg
      481 tcgagtcgcg catggggaaa cgctgtaggc ccacctggat acgtctgggg atgggggccg
      541 gggaggagct aacactccgg aggatcgtcc gtatatccac agccgacagg atcgatccgt
      601 tgaccgagtc gcgccgctaa accggagtcg ccggcgcctg aatggggtgg ccctgctgac
      661 gctgctgatt gcgcgcgcct atcaccgccg ggtctccgtc ccgaatttgt caaatcttat
      721 cgacgagtag tctcgctcgt ttctattgca aaccgccgat ttgctcggcc tggcaagcga
      781 ccagtgcttc tgccgttaag gacccaattg tcaagctggt tgccaaaaaa attatcaggt
      841 gcgcaacttg acaatcgagt gaatcgatgc gaggccttga gtaacccggg cccctgccac
      901 gctcggcgac acagtcgaca cggagcggat aagtgggcct gaccaacacc gagctgcttt
      961 actatgacgc caacgtcctg cgtcttcccg cggacaagcg gtccgagtac cacggacagg
     1021 tcgatcggct ggtcgccgcc ctcagatcgg ccctcgcaga caaatagggt gtgcgggtga
     1081 cccgggtcat gaaggccggc tcgttcgcca agcacacgat cctgcgcaag acggccaacg
     1141 acccggtcga tgtggacgtc gtcttctaca tcgccgagcc agacgccaat cacgatacgc
     1201 tccaggaact caacgacgcg attttcgagc tgttggtcgc ccagtacccg accaaggcgg
     1261 tcgaggactt cgagatccag cgccgtaccg ccaaggtcgt tttcgtcaaa tcgggcctca
     1321 ccgtcgaggt ggtgcccgtc atcgagaatc ctgaccgggc gggctacggc tggcagttcg
     1381 accttgagac gggcgccaag gtcgagacct atccggccgg ccagatcaag ttcgttaggg
     1441 atcggaagtc cgtcgacccc cacttccgca cgctcgtccg gatgggcaag cgctggcgca
     1501 accatgcgga actgacgttt ttcaagtcgt tccagattga gctaatcatg gcctacgtgc
     1561 tggataccca aggcagtgat gggagcatcg accagcggtt ccgaaacttt ctgctgtata
     1621 tcgcccagtc cggtctgaag gagacgatct cctttccgga aaacagtcag ccgattgcga
     1681 gtttcaccgg tccggtcgtg atcgtcgacc cggtctgcag ctggaacaat gtggcggcgc
     1741 gcctcacgga ggaggagagg ctcgaaatca ttgccgccgc gaccgaagcc tgggagacgg
     1801 caaatttcgc cacggtcgag gatgaccacg acctgtggaa ggagctattc ggtccccgct
     1861 tcaagacgga ggccgcataa tgtcgatcag cgcctcccgc tccgcgtcgg aaacgtactc
     1921 ggatatcgac gtcgggaatg tcgtccgccg ggtcggtgcc gatctgagga tgatcgccga
     1981 cagcaccggc tgctggacgc cggtcatgac cgccaactac gtccacgaca tcgaggagct
     2041 cgccaagggt ggttacctca aatttgtcga cgtgaccttg atcagtaacg gtgtcgagat
     2101 cagggcaact cggttctcgg tgatcacaaa cgccggcgac gctgccaacc agcgccccgg
     2161 cgatgcgctt tggccgaaaa tcccgggtgc gacactgcgc atcatcctgt cccacacccc
     2221 ggaatacaat caggctgcgg agaaggcgat gcagttgaag ctccgggcga attgggtctc
     2281 gtcgtctgct gacacgagtc atggcggtct cacccagaat ggcgggcgaa gctacgctag
     2341 caactccttc ggcatgccgc ggacggactg gaaatgagcg acaacaaggt cttcgatagc
     2401 gagatccctc tgccggatga tcgcctctcg gcgcgcgaac ccatgctact tggtttcgac
     2461 gcgcggtatg cccgcgtgca tgatcagcta cgcctcctcc tccaggcgga cgagcttgcc
     2521 gcctggaaca agaagcatca tggcggccga ctggccttgg ccagcctcgc gaccgaccaa
     2581 tatccgctcg tcattttcca cggagacgtc gggaccggca aaacggtgac ggccgagtgt
     2641 attgccaacc gtctggtttc cgaagcgggc gccgacgact cgattctctt caagctcagc
     2701 aatcgagttc ggggaagcgg gttggtcggc gaaatgggca cccagctcgt cgaggcgttt
     2761 cgaaaaatcg agcaatcagc cggcaagcac cggcgtgcaa tcctcgtcat cgacgagggc
     2821 gatagtctcg gcgcatcaag gtctcaggcc cacagccacc atgaagacaa ggtggccgtg
     2881 aataccctga tccaggagat cgaccagctg cggcgccttg gcgggcggat cgtggtgttc
     2941 ctctgcacca atcggctgtc ggtgctcgac cctgcccttc ggagacgggc ggcggtcatc
     3001 gaggagttcc ggcggccgac tgagagcgag cggcatgaac tgctgtcgaa ggatctcgcg
     3061 gccttgaacc tgtcgaagga tgagatcgct cgcttggtgg ctgtgtcggg cgagcgggag
     3121 ggtgatccgc cctggacctt ctcggacatc aggacccggt tctaccctgc cgcgctggct
     3181 atggccttcc cctgctcgcc tttgaccttc gcccatcttg aggctgcggc aacggcaatg
     3241 cgcgcatctc cccgattgga agattaacga tgccgtcgtc atcgctgttt ggtcgtagaa
     3301 tccatattgc cggcagcatt tcgggagatc cggggggcgc ctcgcaggac cagatcgcat
     3361 acgccaggct gctactgcag cgactggttc ctgagctggt tcgtagaggg gccaatttcg
     3421 tgatcccggt ggatcgagaa ccggtgcggg gtgacgggga gaagatctgc ttcgactggc
     3481 tggtctggga gggtgtccac gcagcgttta ctactcggcc gcccgaagct ccgggacctt
     3541 tcatcattgc cgttcagcat cataagagcg aggatcaggt cccggctgag cagtgggaac
     3601 tgtgggaggc tctgcgggcc tcgccggcgg tccagatcga aagcgctgcc cagtggaaca
     3661 tggcgagcaa gcgcatggag gtggcagctc gaaccggcga tatccttatc gccttgggag
     3721 gggctgaagg cgtggattac ctcgcaaatc tataccatga tgccggtaaa cctgtggtgc
     3781 cgctcgacct cccgatcacg ggtccaaacg ccggggcacg aaagctgttc gcttttggcc
     3841 aatcaccgaa aaatgcggcg cggctcttcc gatcgaaggg cgatatgccg ccgcatagtt
     3901 ggctcagccg tatacagcac acgcgccggc agacggccga tgctctggcc tctggcctca
     3961 ccgatctgct cgaggcgctt gctccgccgc gggcgttcgg agtccgcctg ctcaatccca
     4021 atcacccgca ctttgacgcc gtcgaggcac attttgaaac tgtcgtgcgg ccggtaatcg
     4081 aggacgagtt aggctatgag ctcacggtga tcgacggcag ccagccgttc gagcattctc
     4141 gcatcgacca ggaaattttc accaagctcc atcgaagccg ggtcgttctt gcagacatca
     4201 ccgggtcgcg gccgaattgt tttattgagc tgggctatgc cctcgggcgc caacttccca
     4261 ctatggtcat ggggcttgag ggcgccgagc ttcccttcga catcacgacg atctccgggt
     4321 tgatgtggaa ggccgccggt ccggtgaagg agcgccggac agccttccgg aatcactgga
     4381 tcgcgatcaa gaatcgaccc cccattgttc ctgtcgagcc gctcatacca tgaccaagcg
     4441 ccaggaagca ttcgtatcgg tcgacgttga gacctccggg ccaatcccag gagagttcag
     4501 catgcttacg atcggagcgt gcttgatcga tgatccgacg aaaacgttct catgttcgct
     4561 gaagcccatc agcctcaaag ctgatcccgc ggcgatggcc gtgaccggcc tctctctgga
     4621 ccagctcagc gatgccgggc ttccgccgga ggtcgcgatg agacgctttg ccgactggct
     4681 gcgcagccta gctcccgacg gcgatatcgt gtttgtcgga atgaacgccc cgtttgattg
     4741 gtccttcgtg aactactatt tctggcggtt ctatggtcag aaccccttcg gctttacggc
     4801 gctcgatatc aaagccttct acatggggat gacgggatgt cggtgggacg agaccaggtc
     4861 gagcaaaatg gcggaccggc taagtccaac ctccaaaggc acccacgacg ctctgcagga
     4921 cgcgatctat caggccgagc tctttaggct cgcgcgacaa ctggccgtcc ccgacggaga
     4981 ggcctgaaag ctgcccgcaa accccgagac aagcacgtgg cgcctggcac ctcctaaagg
     5041 gtgcaggtgt catccggcag aatggcaaac tacggctgac cccgatctca tccgatcgac
     5101 caacccatgg ccggaccgag tcccgcctcc gggtcggctg gcgtccgact ctgcgcactg
     5161 gggtcaaatt tccagaagct gacgcgatct tcaggcccct gaagggcgat ccccgtggat
     5221 cgccagcgga tctcagctgt tgtgtcaaag cgatcttaga tggctaggaa cttgtcaatc
     5281 acattgactc taacttgcta aattgattca ttgttgtcga atacgggagg caatgccgat
     5341 ggccgaggac atctacgcga gagtgggtgc agccattcga accaaacgcg atgagcttgg
     5401 aatgactcaa gcgtcactcg cggagcggac tggcttaagt cgaacttcga tcacaaatat
     5461 cgaaaaggga ggacaatcag tcctcctcca ccagctcgtg gatatagccc gagcggtgaa
     5521 caccgatcct cgcgattttc tcgttaacct agagcgtgac cagccaaagg tcgcagcacc
     5581 aagttcgcaa atggctgatc ttctgcgtcg tttggatcct ccagcgcggg tcaaacggtc
     5641 atgatcccga gacgagctcg ctattcaaag atagagcgga tagtggaagg cttgctcgct
     5701 cagagcgccc cttcggtgcc cgtccccgtc gagaacatgg tcacggaacg cggcatcgaa
     5761 ctccggtacg gcgaccttga agacgtgtct ggtcttttgg tccgagacgc cgacggcgta
     5821 acgattggcg tcaactcgac gcacagccca gtccggcaac ggttcacgat tgcccacgag
     5881 tttggtcact ttctgctcca tgagggcatc ctagagcacg tggatcgcgg ataccgggtc
     5941 aactaccgat cagcgctgtc ctccgaggcg accgatgtcg aagagattga ggccaatttt
     6001 tttgccgcct cgatcctaat gcctcggtcc tatctcgacg ctcgaagcgc ggttctggcg
     6061 gtcgatgacg acacccaggt aaaggcgctc gcgcaagagt ttcacgtcag ccagcacgcc
     6121 atgagcctca ggctagcaaa cctttatcgt caatatgcgc cctactaaga gctaggtcgg
     6181 gctgccggtt gggagccgtc gttccgcttc cgttaaggct tttcggacgc ctggcaccga
     6241 ctgtcggcgg tgcgccgatg gcaggcgtcc gaaaagcctt aacgatagcg gaagggccgg
     6301 ttacgagcgg gaagacgagg aaagccgcct ttcgcttcgt tcaaggtgaa tgcacgcgtt
     6361 cagaccctcg cttcgttcgg ttggcacggc atagaagctc gtctttctcg tgttgaatct
     6421 cggtcagcaa cgacagcggc aaacggcgaa aactgccggc gctacaaagg ctcggacggc
     6481 gccgaagtca attaacggca ccgatctcca cccgaaaacg gacgttccca acgtcgcagt
     6541 cgagccacca catcgtcggt gcgccggcaa tcaaacccat gccggaaagt tactcggctg
     6601 gactcggcga ccataatcac ctggcgcttg gccttagcag cgacgaacag ggcaaccagt
     6661 tcctcgaaca ccgacttggg gtccaagttc tcctccggct gatcaatgat gaggggccgg
     6721 tcgtccccgt cgtccagcgc cagatatagt aggagaagca cgatgcctcg tgtccccggc
     6781 gacagcttac gaatgtcgat cccgtcatag acgatctcgt agcggacaga gatgtgatcc
     6841 gtgtcgaaaa gccaatgcgc gaactgcttc agccacgcac gaaactccac ctgctggctt
     6901 tgcgcgtagg gtgcgtggcc gatcagatct ctcatgtaat gggcgatgat ctcttctgtg
     6961 aggtcgaggc tgtaggtgag cgctaagtcg agcgacccgt cgtgcaggcc cctgataagg
     7021 tggtcctggt gcgcctcgac agtccgaaac aaaatgcccg cgtgctcgcc ggtgaagcgg
     7081 ctgatcagcc cgggcatcag cggcggcgcc agcgaacgag aaagccgagc gcgatggtgc
     7141 cggtcatgcc accgtccatc tccttggcgg ccatctgcag ctcttcagcg attttgagca
     7201 gattgcgcgc ctgcccgagc agatcgcggc ccgcctgcgt cagcgacagc ccgctcgcgt
     7261 gatgccgaat gaagagctgc acgtcgaacg aggcttcgag atcggcgagc gcgctcgaga
     7321 tgacggctgc gaaatgccga gccgacgtgc cgcggcagtg aaggacagcg cttccgcggt
     7381 gacggtgaaa tagcgtagct ggcgcagcga gtagcgcaaa ggagggcttt ccattggcga
     7441 cgctccctat gggcaaatag cgcgcatagc taaatcctac tctatgcatc ttttaaatat
     7501 gcttttcgca tttcgagcgg tcgcatagct tcttgcgaag acaaagaacc agggcggcag
     7561 ccatgacagt ggaaacgacg tcaatcgaca cgctggttgt cggcgccggt caggccggtg
     7621 tcgccatgag cgagcatttg agggaactcg gcgtgccgca tctagtgctg gagcgcgacc
     7681 gcatcgccga gcgctggcgc acgggtcgat gggattcgct ggtcgccaat ggtcccgcct
     7741 ggcacgaccg tttccccaat cttgagtttc ccggcgtcag cccggacggg ttcgcctcga
     7801 aagacgaggt cgcggagtat ttcgtcgcct atgccaggaa gctcgccggt cccatccgca
     7861 ccggcgtggc tgtgacgaag gttgtgcgca atgccgaccg gccgggtttc atcatcgaga
     7921 cctccgatgg cacgatcgag gccaaccgcg tggtcgtcgc gaccgggccg ttccaacgtc
     7981 cgctcattcc cccgatcgcg ccgaaggcgg aggctctcat gcagatccac tccgccaact
     8041 atcgcaaccc cgggcaattg cccgacgggg ccgttctggt ggtgggggcc ggatcctcgg
     8101 gcgtgcagat cgcggacgag ctgcagcggg caggccggca ggtctatctg tcggtcgggg
     8161 cacacgatcg cccgccacgg gcctatcggg gtctcgatta ttgctggtgg ctgggcgtgc
     8221 tcggcgagtg ggacaccgag gtcaagaagg cgggccggga gcacgtcgcc attgccgtga
     8281 gtggcgcgcg cggtggccat accgtggatt tccggcgcct ggccgagcag ggcatcacgc
     8341 tcgtcggctt gaccaagtca ttcgaccacg gcgtggctct gtttgaagcg gatcttgcga
     8401 cgaatatcgc caacggcgac gagaattatc tcgcggtgtt ggacgccgcc gacgcctatg
     8461 ccgtccgcaa cggcctcgac ctgccggaag agcccgaggc ccgcatcatt cccgcagatc
     8521 cccactgcct gacccatccc ctactcgagc tcgatctcgc gaaggccggt gtcacttcga
     8581 tcatctgggc cacgggctat gccacggact tcagctggct ccaggtcgat gccttcgacg
     8641 aaaccgggaa accgaagcat cagcgcggta tttccaaaga gcccggggtc tatttcctgg
     8701 gattgccgtg gctgtcgcgc cgcggctccg ccttcatctg gggcgtgtgg catgatgccc
     8761 ggcacatcgc cgaccatatc ggcatccagc ggcgatatct cgcctactac gaggcccgca
     8821 cgacgccccg ctagcatcaa ggtgcatgat gagccaaccg acgcataccc gtatccgcat
     8881 gttcaatacc caggacacct atccgaacca gaggctcgac aacgatctct gtcaggccgt
     8941 gcgcgccggc aacaccgtct atgtgcgtgg ccaggttggg accgacttcg acggcaatct
     9001 ggtcgggctc ggcgatccgc gcgcccaggc cgagcaggcg atgaagaacg tgaagcagct
     9061 tttggcagag gcgggcagcg atctcacgca tatcgtcaag accacgacct atctcaccga
     9121 cccacgctat cgtgagccgg tctatcagga ggtcggcaag tggctgaagg gcgtttttcc
     9181 cgtttcgacg ggcttggtcg tcagcgcgct cgggcagccg caatggttga tggagatcga
     9241 cgtaatcgcg gtcatcccgg acggctggac atcatgacct tctccattgt cgcgcgctgc
     9301 ccgcgctccg ggcgcttcgc cgttgccgtc gcctcctcct ctcccgccgt ggcggcgcgc
     9361 tgtgcccacg cccgggccgg cgtcggcgcg gtcacgacgc agaatgtgac ggatccgcgg
     9421 ctcggcccgc agatgctcga gctgatgcaa ctgggcgcca gcgcgcggga ggccgtcgac
     9481 atcgtccggc ggtcagcccc gcacatcgag caccgacaat tgacggccgt cgacaaggac
     9541 ggcgcgactg ccggtttttc cggcgagcgt gcgctcggct gccatgcgag cgcggagggc
     9601 cggaatgcgg tcgcggccgg caacctcctt gcggaccccg gggtgccgca ggtcatggtc
     9661 gaagccttcc aggccggagc ggccgagggc gatctgggcg atctcgtcat cgcggcaatg
     9721 caggcaggcc ttgccgcggg cggcgaagcg ggcccgatcc ggtcggccgg cttcctgctg
     9781 gtcgacaggg tcgcctggca cgttgccgac ctgcgcgtcg attggcacga ggagccgctc
     9841 gccgagctcg cgcggctgtg gcagctatgg cggccgcaga tgcaggccta tgtggatcgc
     9901 gccctcgacc ccgcggccgc acccgcatac ggcgttcccg gcgacgaata gaaggcgggc
     9961 aggcgcggag cggggtggca ctcgggtgcc gggcatatga cgcagacggg ccggcagcct
    10021 attccgcgcc ggcggctcgc ccagccgcgg cgcggtccgg agccgggcct cacgcgtgcg
    10081 ccttgcgcgc cgccaccgcc tctgcgagga cgcacaggat ctcgtacatc atcgaggccc
    10141 cgacgagcgc ggtattgccg ctcggatcga agggcggcgc cacttcgacg acgtcgccgc
    10201 cgacgaggtt caagccgcgc aggcggcgga ccatgatctg ggcctcgagg acgctcatgc
    10261 cgccgcattc cggcgtgccg gttcccggcg cataagccgg atcgaggccg tcgacgtcga
    10321 agctgacata ggtcggtccg tcgccgacga tcgaccgcgc ctcctcgatg accgatttgg
    10381 gcccgcggtc gtagaactcc tccatataga cgacgcgcat cccgctctcg tggctgaacc
    10441 gccagatatc gcggtcattg atgccgccgc ggatgccgat ctggatgacg cgcttgggat
    10501 cgagcaggcc ttcctcgacg gcccggcgga acggcgcccc gtgatggaag cgcgagccca
    10561 ggtaatcgtc gcccgtatcg caatgggcgt cgaaatggat catgccgatc ggccgttcct
    10621 tcgcgatcgc gcggaagatc ggcagggtga tcgagtggtc gccgccgacc gaaagcggca
    10681 cggcactggc cctgtggagg ccggcataga aggcctcgat ctcgcggtgc gccccttcaa
    10741 gctcgtaggg cttggcaacc cacgcatcgc cgatatcgcc gacgcggcag agcgcgtagg
    10801 gatcgatgcc ggtcgcctga ttgatccggc gcatcaggct cgactggttg cgcacctcgc
    10861 gcgggccgtg gcgggcgccg ggccgattgg tgaccccgcc gtcataggga acgccgacca
    10921 tggcgatgtc gagtgcaccc ggatcctcga cgaacggcgc ccgcatgaag gtcgggatgc
    10981 cggtgtagcg aggctgtacc atccggtcgg agaactcggg cgggttcggt tcgccggtca
    11041 tcatccttgc tccgcttcgc tcgccggaca tccgggcgtc agttcgaaag tccttttgca
    11101 tgaaagatga atccgagcag attcatcagc aattgcgccg cgaggaaggc cgtcgtgccc
    11161 gcatgatcat aatccggggc gacctcgacg aggtcaatgc cgaccacgtc gccctgtccg
    11221 gtcaagccct gcaggatctc cagcacttcg taatagagaa agccgccatg gctcggcgtc
    11281 cccgtgccgg gcgcgatcga cggatcgaag ccgtcgatgt cgatcgtcag atagtagcgg
    11341 gcgccacgtg gaatgcgcgc aagcacctgc tccttgccga gccggcgcac gtcgcgcacc
    11401 gacaggatcg tcgagccggc ggcgcgggcg gccgcataat cgttgcggtt cgacgacgag
    11461 acgttgcgga tgccgagctg cgtgagcccc gtgacatggg ccatttcgga ggcgcggcgc
    11521 agcggattgc catgcccgaa gcgcacgcca tggcggtcgt cgacgaagtc gagatgcgca
    11581 tcgaaatgga cgatgtggac tggcggctgg tcggcaaagg cccggatgca cgggatatgc
    11641 accgagtgat cgccgccgag cacgatcggc agcgcgccgg cggccaggat cttgcggacg
    11701 cccagctcga tgttggcgtg gctgcgcatc gtgtcggtat ggatgatgtc ggcatcgccg
    11761 atatcgacga tgcgcacctt gtcgaccggc agataggtga cgtcgtcctc gaaatcgtag
    11821 gcgccgccat ggccgaacga aaacagcgtc gaggcttcgc ggatagcgcg cggcccgaag
    11881 cgggcaccgg cgcgatactg ggtgcccatg tcgaatggcg caccgaggat cgcgccatgg
    11941 ccgtcgatcc gatcccaatc caggcagatc gggcgcttgg cgaagctgca atgcccgacg
    12001 aagggcaggt tgagccgccc gctgtcatat tcgttctggg ccatcgcctg cctctccgcc
    12061 aagagccgcc gccagcgcga tcgcatggac tgccgggcgg cagcatgcgg caatcgcata
    12121 tacggtggca acaccggtgc cgtccgccgt gccggcaaat cgagaacgcg ccgagcggca
    12181 gacacggccc gtgttgctca tctcgcgcca cgaaatgcat gcgccttgca tatttcgcgc
    12241 gctggagcgc cgtccggcaa gcccgaacga actgtttacg actgtcgaag tccgggacta
    12301 ggctcatgct ccaaagcggt gcatcattat aggtgcccag cgcgtgcccg gcagcacttg
    12361 gcatcgccct tgcgaggatg ccggtggagg cgctgagcag agggcggtcg gcgatcgccc
    12421 cgtgatccgc aagccgggac tgtcgtcgcg atatcggtgg agcagcacct tcaaactctc
    12481 gataaataaa aacgggggct cacgatgcgt aggttctttg gtctgctcct tgccgcgacc
    12541 gcggcggtgg tctcctgcgg cgcgctctcc ggcccggcgg cggccgcgga caccgtcaag
    12601 atcgcccatt cgacctgggt cggctacggc ccgctctaca tcgcccgcga caaggggatc
    12661 ttcaagaaga acggcgtgga cgtcgacctc gtcgtgcttg aagaccccaa ggaacgcttc
    12721 ccggccttga tggccgacca gatccagatg atcgccagca cggtcgacac ggcgctcctc
    12781 tatctgaagc ggccgaccga cttccaatat gtcgtcggga tcgacgattc gaagggcggc
    12841 gacggcatcg tcgcgatcaa ggacatcaag acggtcgccg atctcaaggg tcatacggtc
    12901 ggcgtcagcg aaggttcggt ctcggagttc tatctcaacg tcctcctggg ccaggcgggg
    12961 ctcaaggaat ccgacctcaa gacggtgaac atgaccgcgg cggacgccgg cggcgccttc
    13021 gtcggcaagc gcatcgacgc ggccgttacc tgggagcctt ggctcagccg cggcaaggcg
    13081 accgatttcg gccatctgct cgtcgacagc tcgacgacgc ccggcctcat caccgacgcc
    13141 gtcatcgtca agaccgattg gatgaaggcg cacgagaagg acgtcgccgc catcgtcaag
    13201 tcctggaacg aggcggtcgc ctattatcgg gaacatccgg acgaatccat cgagatcatg
    13261 gccaagggcg tcggcggctg gctcaaggat ccaaaggact tcaaatcgac gctgcccggc
    13321 atcaaattct atggcgccga ggacaacaag gtgttcttcg gtaccaagag caagcccggc
    13381 ccgctttaca agacggtcga gtcggccatc gacatctggt cgagccacgg caaggtccag
    13441 gtgaaggtga cgccggacga cctcatcaac tacagcttcg tcaacgagtg atggcggcgt
    13501 tcgaagagta cacggcaccg aagaccgaga ctccgcggca acgactgatg gcggcgttcg
    13561 aagagtacac ggcaccgaaa accgagatcc cgcggcgtgt ctaccttacg gtcagcacga
    13621 tcgtcgctgc ggccctgctg ctgctatggt gcgtcctcgc ttatggcaag ttcgtacgcg
    13681 aggatttcct gccgaccccc gtcgcggtcg tcgcggcggc ggcggcgggg atccgcgaca
    13741 attcgctgat cgtcaacacg gcggtcagcg tcagcgagat cctgtcgggc ttcgttctcg
    13801 cctcgatcat cgcggtgccg ctcggcatcc tcatgggcag cttcaagatc gtcgaggcgg
    13861 cgatcgagcc cgtgaccaac ttcatgcgct acctgccggt ctcggcgctc attccgctgc
    13921 tgatcctgtg gatcggcatc ggcatcgagg aaaaaatcgc ggtcatcttc ctgggcacgt
    13981 tgttccagca gctgatcctg atcgcggacg tctcgcggct ggtgtcggcc gatctcctcg
    14041 acgtctccta tacgctgggg gccaaccggc gggtcgtcat ccgccgggtg ctgatcccgg
    14101 ccacgatgcc cggtgtgatg gacacgctgc gggtgacgct cggctgggcc tggacctatc
    14161 tggtcgtcgc cgagctggtc gccgcgaaca agggcctcgg ctacttcatc ctcaactcga
    14221 tgcgcggcct gttcaccgat cagatcttcc tcgggatcat cgtcatcggc ctgctcgggc
    14281 tcgcgaccga ccagctattc aaggcgcttc gttcatggat gcttccctgg gcaaccacag
    14341 cctgagcctg ccgggccgca gcccggcggc caagctgcgg atcgacaagg tcacgctgcg
    14401 cttcacgccg cgcagcggca atccggtcac ggccctcgac ggcatctcgc tcgatgtcgc
    14461 ggatcaggag ttctcagtca tcgtcggccc ctcgggctgc ggcaagtcga gcttgctgcg
    14521 gctggtggcc gggctcgcgc ggccgaccgc cggcgcaatc catctcgacg gcaaggcggt
    14581 caccaagccc ggcaaggacc gcggcatggt gttccagtcc tatacgctgt tcccctggct
    14641 gaccgtccgt cagaacgtcg aattcggcct ggaggtcgcc gggctcgacg ccgcgacgcg
    14701 ggccaggatt gccggacgct tcatctcgca ggtcgggctc gacgcattcg tcgatgccta
    14761 tccgaagcag ctttcgggcg ggatgatgca acgcgtggcg ctcgcacgcg cgcttgcgaa
    14821 cgatcccgag atcctgctga tggacgagcc gttcggcgcg ctcgacagcc agacccgctc
    14881 gctgatgcaa gagctgctgc tcaatatctg ggagagctcg cacaagacgg tgctcttcat
    14941 cacccacgac atcgacgagg cgatcctgct cggcgaccgg gtctatgtga tgaccgcgcg
    15001 gccggggcga atcaaggagg tgatcgagat cgacattccg cggccgcgga ccgtcgaggt
    15061 tctcacctcg gcgcccttta tgcagttgaa gcggcgcatc atggcgctga tccacgagga
    15121 agccgtgcgg gccatgggct aggagggccg cgtcgcggca agggcacggg ggtgccgaca
    15181 cccgcaggtg ccgccttggg tcgaagccgc tgccggcgga ggatgcgggc gatcacgggc
    15241 ccggatttga gcgcctaccg cctgatggtt tcgtgcgcga cctcaaggcc gtgctcggcc
    15301 gacagttcct cgacgttccg gcagttcagc gtgccgccta ggcacaacca gatcgcgtgc
    15361 ccgacgccgt cagtgcaccg agatccgata ccgagacccg atcaagaggc tttggcgttg
    15421 ttccgcgagg tcagcggcgc gcttcatacg ctagcactac tttggcacat ttttagcgac
    15481 gggcgagttt ttgggattcc caagagaagg tttgcgtgat tcatgagagg tcggcttatg
    15541 gagggccgat caatggacgt ggactggcaa gccgatctcg aacgttggct tgcgccgttt
    15601 gtcgcggcgc tacggcacaa gacgcgggcg cggatgtgcc ctctctatgt cgcgggtttg
    15661 attggtccag gagaccgcaa gagcgttcag ccgatggcgg cgcgcgctgg cgaggtcggc
    15721 tacgatcagc ttcatcattt cgtcgcggcc ggcgtttgga acagtgctcc cctcgaggct
    15781 gccctgctga aggaggcgga tggcctggtc ggcgatgagg cggggtttct cgtcatcgat
    15841 gacaccgcgc tgcccaagaa gggacgtcac tcggttggcg tggcacccca atatgcctcg
    15901 tcgctcggga agactgccaa ctgccagtcg ctggtctcgg tcacgttggc gtcgcgtgag
    15961 gtgccggtga tggtgggcct gcggctcttc ctgcccgaga gctggacaga tgatcctgag
    16021 cgcatggcgc gagcccgtgt gccgaagcat agacagactg ctctgaccaa gccggagatt
    16081 gcgatcgagg agatcgaccg ggtcatcgcc tccggtgcgc gtttcggctg tgtgcttgcc
    16141 gatgcggggt acggctccag cggacctttc cgtcaggctt tgagcgagcg cggtctgctg
    16201 tgggcggtgg gtctgtcgcg acgccagaac gtctatcccg ccgacgttgg cctgatcttc
    16261 cccgtcgcga aggccggaaa gccccgcaaa catcacatcc ccgatcaacc cccggtctct
    16321 gccgaagcgt tgctggccgc agcgaaatgg caaaaggtca gttggcggcg aggcaccaaa
    16381 ggtcggctga catgtctctt cgcggcccgc cgtgtccgtg ttgcggacgg ccataagcac
    16441 cggatgctcg ataaccgtat gcagtgcatg cctggcgacg aggtctggct cgtcggcgag
    16501 cgacggtcga ccggggagca aaaatactat gtgtcgaacc tgcctgccga tgccgccatc
    16561 aagacgctcg ccgccgcgat caaggccagg tgggtctgtg aacaggccca ccagcagctc
    16621 aaggaggagc ttgggctcga ccacttcgaa ggcagatcct ggacgggatt acaccgacac
    16681 gcattgatga cgatgatcgc ctacgccttc ctgcaatccc gccgcctcaa ggcagtggga
    16741 cggaaaaaaa agagtctcgg gaccgccacc acaaccaagc atgccggcca tcaggcaagc
    16801 gatcctcgac ctattcgcgc gaccgccatc ccgatgatgt ccgcactgtg gcagcctcct
    16861 ggcggatgtc gcgaaaccta aagtgccaaa gtagtgctag gtaggggact catttagccc
    16921 gctgatacca gaaccctggt cgtgctgcgg ccatcgcttt ccgatggaga tcaccatctt
    16981 cgtcgggaag gcgctgttgg cgccacgtcc gcttccaggc cgccgatgtg agtggccgga
    17041 tgaccgtgat gggcgcgatg ccgcccgacc gctcttcgac ttgggcgggc cgagagcgga
    17101 agggcagctt acgggtgctg ccatagcagt cgctgccctt cggaagtcgc aggacggaag
    17161 gcggcttgcg acccgtaact gacccgtgct gacgcgaggc attcccttgc gccgtgacgg
    17221 ccgtgggtga tcacgccacc ttcatctcga atgtaagatg atcgggtgcg agcgctgcga
    17281 gcacgtcctt ggcatccagg atcgcgccgg gtgcgtgcgt gccgccgctg ctgaactcgc
    17341 ccttgagcag gcgctctgcc gcctcgcaga cgagcggcgc cgaaaaggcg tagatatcgc
    17401 gccctcgcgc gacaacacgg cggctctctc cggcatgttt tacgacgacg tcgaccacga
    17461 agcgttgcgg cgagcgccct gtcgcatccg ccggctccgg ggcaggggtc gccgaatcgc
    17521 ggatgtcgct gagcgcgttg ctgctgagat atgtgtggag ctcggcggtc ttcacatgcc
    17581 gcgcgatcag gatgatctcg gaaaaaggca cttcgaccac ggcctgacgg ccgagcggat
    17641 cgccgaactc ccaaagcttc tcggcggctg gcaacgcgac aggcaccagc cgcccctctg
    17701 agatcaccac gcgccgcgcc gtattcctct cgcccgtaat gcgcgtgccg cgcgtcggat
    17761 gccagctgtc gagaccgatc atgatctcga tcgtgtctgc attgtcccag tcgcccagtg
    17821 cggccgtgac cagcagatcg gcgaaaccgc catagaaccc catcgccggg atgagagcga
    17881 tgcccgcatg gcgcgcggcg gcatcgtagc tgtcgagcgt ggcccgcgca ctgggctgct
    17941 ccgcggtgac gtcgagataa tggatgccgg cgcgcagcgc agcggacacc acggcgtccg
    18001 ccgtgtcgag aaagggaccc gcgcagttga tgaccgcttg ggctccgtca agcgcccggt
    18061 cgagcgacgt cgcatcatcg gcggatgcct ggcggcgttg gatttcgtcc ccggggaagt
    18121 tcgcggtgag cgccgcggtg tcgcgggcaa tcgcgatcgg tacgatcccg cggcgctgca
    18181 actcagcgac aacgaagcgc ccggtgtgcc cggccgcgcc gaatatcgcc actgccagtc
    18241 tgttcgctcg cctgctgttt gtgttcatcc tcaattccct ccccatgcgc tacagacctg
    18301 tctgttctat cgatacgtac aggtctgtag cgcgtcaagc gcgaatgtgc taatgagccg
    18361 atctggagac accaatgccg aagactgcag cgatttcaga cgcacacagc actgccgaag
    18421 tcagggcgag aatcctcgag accgcgtgca tgctcttcta ccaacgtggc gtgcgggcgg
    18481 ttggcgtcga tctcgtcgtc gaaaaggccg gcgtggcgaa aacgagccta taccggcact
    18541 tcggcaccaa ggacgatctc gtcgcggctt ttctcgagcg cgaggacgaa gatttctggg
    18601 ggacctggga tcgcgtcgcc aaccggcata gcgacgatgc cgccggcgaa ctggacgctc
    18661 accttgagtg gatcggcgaa cgagtcggcc gtaccaacta tcggggctgt ccgcagatca
    18721 acgttgccgc ggaattcccc gagatcgatc atccagcgcg gaaggttgct gccgcgcaca
    18781 agcgccagat gcgtcagcgc ctgaaacgca ttgccgagcg attgggagtc gcccgcccgg
    18841 atgaacttgc cggtcaactc tcactgctca tcaatggcgc atttgtgagt tcacagattc
    18901 tcgaactcgg cgagccaacg cccctgctcc gcagcgcggc gcatgcgctg atcgaggcaa
    18961 gccggactta gcgccttcca ccgtgtatcg tgcccccgcc cgcgatgccc atcacgagcg
    19021 gccgccctgg attcgcgcga tagcacctcg ccctcagatc attctcgcca agaataagtc
    19081 ctagtcgagg ggcccgccta cccggtgatc ggcctgaaag tcatcttccc tccgtgatcg
    19141 ccgctcggcc ggcagccaga ccaggaggga tcgacaaatg acgcttcaag ccaagctcga
    19201 cgctttcaag gccgatttcg aagcgggcaa gcccccgtac agcgtgccgc gttctgtgat
    19261 tgcgatcatg catcgcgcca cgaaggaact gatcgcttcc ggcgccgccg agcgcgcgct
    19321 caaggtcggg gataaggctc cggccttcac gctcaaggac ccggagggca atgaagtttc
    19381 gtcgctggcc ctgctccaga acggcccgct ggtcgtcagc ttctatcgcg gcgtctggtg
    19441 cccctattgc aacatggaac tgcaggcact cgaggcggca gcaccggcgt tccggcgcct
    19501 gggcgccagc ctggttgcga tctcgccgca gaccgccccc aacagccgca agtcggtgcg
    19561 ccagaacagc ctgagcttcc cgatcctgtc ggacacgcac aacgatgtcg ccgccgcctt
    19621 cggcctgcgc tttgcgctgc ccgactatct ggccgagctc tacaagaacc ggaagaacga
    19681 cctgccgaac ttcaacggcg acgacagctg gaccctgccg atgccgggcc gcttcgtcat
    19741 cggccaggac ggcacggtgc tctatgccga ggtcaatccc gactataccc gccggccgga
    19801 gcccgaggac atgatcccgg cgctcgagcg ggccagcatc gtccacgcct gaagacccat
    19861 ccgcgccgcc ctctccggcg aggacggtgc ggcctaggag gaatgaaatg acaagacgca
    19921 gcttcctcat taccggcgcc agcaagggca tcggccgggc actgtccgcg cggctcacag
    19981 ccgcgggaca ccaggtcgtc ggcctcgccc gcaatgccga cgcaagtttc cccggcgttc
    20041 tggtgccggt ggagctcggc gatcgggccg ccacagcggc ggtgctggcc gacctcgtcc
    20101 agcgcttcga gttcgacggc gtggtgaaca acgtcggcct ggtgcggccg cagcggttgg
    20161 gggacatcga cctggacgac ctcgacgccg tgctccgggt caatctacat tccgccatcc
    20221 agaccgcgca ggcgcttctg ccgcagatga aggcgaaggg ctggggccgg atcgtcaata
    20281 tctcgagcct gacgatcctc ggcatcgtcg agcggacggc ctatgccgcc gccaaggccg
    20341 ggctcgtcag ctttgcgcgc tcctgggcgc ttgaactggc gcaaaccggc atcacggtca
    20401 attccgtggc gcccggtccg accgagaccg agctgttccg ggcgaacagc ccgcccggca
    20461 gcgagggcga acgccgctac ctgtccgtcg tgccgatggg gcgcttcggc aagcccgacg
    20521 aaatcgccgc ggcgatcgcc tttctcctgt ccgaggaagc cggcttcatc acgggccaga
    20581 cgctgttcgt cgacggcggg gcatcgatcg gcaaggcctc gatctgaggg gcggcgcggg
    20641 cacggcgtca cggtcgcaag ctcgtcagaa gcgtactacg cggtcagcgc accgacgtgt
    20701 tccgcgtgcg gcagagctcg ctggaggtgc atcaaggaga tcaagccctg gctcgaacgg
    20761 gcgggcaaca cagtctcctc gctcaagcct cgtctcccgg atagcgcggt ggcgaccccg
    20821 gacgatcggc cgggccgcaa agctagcggg cttcgacgac ggcaggctaa gctcgggccc
    20881 ctgtcttcgc ccgcgttccc gaggtgccat gtcccgccgc ctgccgccgc tcgccgcgct
    20941 tcgtgccttc gaggcggccg gacggctcct gagcttcagc aaggcgggcg acgagttgct
    21001 ggtgagccag agtgcggtca gccatcaaat caggcagctg gaggctagcc tgggcgtgcc
    21061 gctgttccgg cgcgaggtgc gccgggttag cttgacgccc gagggcgcaa cctatttcgc
    21121 aaccgtgcgc cgggcattcg atctcctggc cgaaggcacc gaggctgtcg cagcacccac
    21181 caaggcaacc gtgcgtgtca gcctgctcgc gtcgttcgcg acccattggc tggtgcctag
    21241 gctcggccgc tttcgcgcgc gtcatccggg cgtggccctc gcgcttgagc cgtcgatcgc
    21301 gctggtcgat ctggagacgg gcggtgccga tctcgcgatc cgctacggcc ggggcggctg
    21361 gccgggcgtc aatgcgcgca agctcatgga cgaggccgtg tcgccggtcg cgagcccggc
    21421 gctcatggcc cgcggacccg ccgacgcgcc tggagaccta ctcggccacg agctcctgtt
    21481 ctcctattcg ccgcggctct tcgagtggga gagttggagc cgggccgccg ggctcgatct
    21541 cgggacggcg cggcgcagcc ttctgcatga ctacaatatc gtgctgcagg ccgccctcga
    21601 cggtcagggg atcgcgatgg gccggcgccg gctcatccgg gatgagctcg cggccggccg
    21661 tctcgtccag ccgttcgcgc tccagatccg cggggatatc gcctggtggc tcgtgctgcc
    21721 gcgccgcaag ctcggcccgg ccgcccaggc cttcgtcgac tggctcgatg aggaggccgc
    21781 cacggatgaa tttgattcat ccattgcggt aacgctttga attggtcgcc ggcggtgtgc
    21841 acggcctagc atccctgccg gatcatccac ggacagagct tcacccatga tcgacaccat
    21901 cgccgcccgg ctcgagcgcc tgggccatcg cctgcccgag gtatcgggcc cggccgccaa
    21961 ctacgtaccg gtgacgcgcg tcggcgcgct cgccttcatc tccggccagg tggcgccgtt
    22021 cgccccgggc gacgagccgg gcgcccggct cggcgccgaa ctttcggtcg aagcggggca
    22081 gaaggccgcc gctgcggcgg cgctctcgct cttggcgcag atcgaccagc tcgtggcagg
    22141 caatcccgcg ggggtgagga gggtggcgcg gctcggcgtg ttcgtggcgg cgacgctcga
    22201 gttcaaccag cactcgctcg tcggtaacgg cgcctcggac ctggtcgtgg ccgtgctggg
    22261 cgaggccggg cgccacgcgc gcactgccgt cggcgtcgcc tcgctgccgc gcggggcagc
    22321 ggtcgaggtc gaagccatcc tcgaactcgc ggagtagagc tcatgggcct cgatattgcc
    22381 aagctgcgca ccgaaacgcc gggtgccgac cgggtgctgc acctgaacca tgccggcagt
    22441 tcgctcatgg cggccggaac cgtggcggca atcgaggacc agctgcggcg tgagatggaa
    22501 gccggggcga tggaagcggg cgccgccgtc gccgcccgaa tcgatcaggt ccgcgctgat
    22561 atcgcgaccc tggtcggtgc cgcggccgac gagatcgcgt ttaccggcgg caattccgac
    22621 ggctggggcc gcgccttcgc cgccctccgc ctcaagccgg gcgaccgggt gctagtcggc
    22681 cgctccgaat ggggcggcaa cctcgcctgc ctcacgcgcg ccggtctcgc gatcgagacc
    22741 gtgccggccg acgaccatgg ccagatctcg ctcgacgcac tcgaacggat gctggacggg
    22801 cgggtgcggc tcctggcgct cacctgggcc ccggccaacg gcgggctgat ccagccggca
    22861 gcggcggtcg gccggctcgc gcgcgccgcg gaggtgccct atttcctcga cgcggcacag
    22921 atcgtgggcc agtggccggt cgatgtcgcc gaactcggct gcgacgtgct gacggcgccg
    22981 gcgaggaagc acctgcgcgg cccgcgcggc gtgggcttcg tctacgtccg gagcggcttc
    23041 gcggcccggc tcgagccgac cttcgtcgat acccaatcgg cgccgatcgg cccggacggt
    23101 gctgtgctaa agccccatgc gcgccggctc gaatcggcag agggatcggc cgccctccgc
    23161 ctcggtttcg gcgccgcggt gcgccagacg ctcgaactgg gcgtggaccg gatccgcgcc
    23221 gcggtcgccg ggcgcgccga gagcttgcgg tcgcggctcg cggccatgcc gggcgtgcag
    23281 ctccgcgacc tgggcatcga gggctcgggc ctcgtctcct tcacgctcga tggccgatcc
    23341 ggcgccgacg tgaagaaggc catggcggcc caaggcatca acctaggcct caacggccgc
    23401 gcctttacgc cgctcgacat ggacgcgcga ggcctgaccg aagtgctgcg cgcttcggtc
    23461 agctatctca cgaccgaggc ggaactcgac cgtttccagg cggcgctcgc ggccctcgtt
    23521 ggctgagagg cgccatgcta caattcagac gagtaacagc ttgagactga atatccgctt
    23581 cgaggcatcg gcgccaatca atcgaatggc ctgggtg
//
LOCUS       DATGEM010000607         5435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1439486, whole genome shotgun sequence.
ACCESSION   DATGEM010000607 DATGEM010000000
VERSION     DATGEM010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439486"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VKQ27_18725"
     CDS             <1..785
                     /locus_tag="VKQ27_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91025.1"
                     /translation="AADTVALAHFLTMRASGEFAPVPELYAVLHMRPYCDDPAAADDP
                     FQHYLADMLLAKREMFPDFDVIAASGVIDPNYYLINGSDVHEAHLDPVEHFCRYGWRE
                     WRKPNIYFDMHWYLHTNPVVERMKINPAVHYVLEGEAMGRRPVPYFDPLWYRETYAIA
                     PEQNALAHYLTHRRSQKFSPTPMFNVAWYVDQHPEDVRGNRDPFAHFLQVGTYRDLDP
                     SPTFNAAEYRKRHIGRPSRKFRHLMHPDRDNPLVHFLRAGYR"
     gene            complement(864..2660)
                     /gene="phaC"
                     /locus_tag="VKQ27_18730"
     CDS             complement(864..2660)
                     /gene="phaC"
                     /locus_tag="VKQ27_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HLY91026.1"
                     /translation="MAETQKTETGFKLPDPADIGRSMADIAERSQRIVSEWLKRQADE
                     NHSPDPLNIASAFMEMTARLIANPSYLVEAQIGFWRDYMSLWQNTTRRILGADTKPVI
                     DAPSSDRRFKDDAWRENEIFDFIKQSYLLSARYVQDVVTHVDGLDAKTAQKVDFYARQ
                     FVDAMSPSNFLLTNPEVLRKTAETGGENLLKGLNNLLSDLEQGKGKLRIKMTDMEAFR
                     LGENIGVSPGKVVYQNDLMQLIQYTPSTEKVLKRPLLIAPPWINKFYILDLRPRNSFV
                     RWAVSQGHTVFMISWVNPDAKLAEKNFEDYMQQGYLDALGAIEQATGEREVNAIGYCL
                     GGTLLTSTLAYMAAKKDDRIKSATFFVTLTDFEDAGELGVFIDEEQLKSLEGKMNKRG
                     YLEGSEMATTFNMLRANDLIWSFVVNNYLLGNDPFPFDLLYWNADSTRMPAKMHSFYL
                     RKMYQENLLSKPGGISLAGVPIDLRKIKTPAYFLSTREDHIAPWKSTYRGTQLLGGPK
                     RFVLAASGHIAGVVNPPESGKYGHWINKDLPPDPEEWFKGATEMAGSWWPDWHRWVSA
                     LAKQQVQARVPGDGKLKPIEDAPGSYVKVRLS"
     gene            2805..3956
                     /locus_tag="VKQ27_18735"
     CDS             2805..3956
                     /locus_tag="VKQ27_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91027.1"
                     /translation="MSGLRRCMAPAALVLLLGGCSSFPSSMNPVSWWHNLEGGEIAKE
                     RPPPPGATEPYPNLASVPPKPEAPDQATLENITNALVADRTNAQHLADAAPLADPSSP
                     SASPALFGKGTLPPPPPAPPPGTQTTSANLSAAEAPPAAPTTPAAPSPTTPAAKPSGT
                     AEEIAPPPAKAPVGDVQTAPLAPPGAAAQPAAQSAAAPPLPTTPPPPPSLPGVTAAAR
                     TPPAASASAPAPMPPVAAVAPPRAAPPPVPPAPAPQPARTVPPPPAAAPPSAPTTVAS
                     ATPPPAKVPVPASAISLTFIAGSADLPALAAGQLKQLAGRRGNGIIAVTGYGDAASND
                     PDAQAAALTLGLSRAKAMAAALTADGVPASAVQMDAEAIGRGGTARLVQ"
     gene            3980..5209
                     /locus_tag="VKQ27_18740"
     CDS             3980..5209
                     /locus_tag="VKQ27_18740"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006559331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HLY91028.1"
                     /translation="MSSDPSGEFHRIRRLPPYVFAEVNTAKAKARTAGDDIVDLGMGN
                     PDSPTPPHIVAKLVEAVQDPRTHRYSVSKGIPGLRRALAAYYQRRFDVGLDPETEVVA
                     TLGSKEGLANLAAAITSPGDTILVPNPSYPIHQFGFIIAGAAVRSVPAAPDEEMLRAL
                     DRAVRHSVPKPTALIVNFPSNPTAYLADLDFYREVVAFARRHEIWILSDLAYAEIYFG
                     DNAPPSLLQVPGAKEIAVEFTSLSKTYSMPGWRMGFAAGNPRLINALTRMKSYLDYGA
                     FTPIQVAAATALNGPQDCVDQIRSLYRERRDVLIRGLASAGWDVPNPDGSMFAWAPIP
                     ARFAGLGSVEFSKLLLARAKVAVSPGLGFGEHGEGYVRIALVENTHRLRQAVRNIRAF
                     LQAGTNQAEAEVVSVAS"
BASE COUNT          900 a         1733 c         1745 g         1057 t
ORIGIN      
        1 tggcagcgga taccgtggcg ctggcgcatt tcctgaccat gcgagcgtcg ggcgagttcg
       61 cgcccgtgcc cgaactgtat gctgttctgc atatgcggcc gtattgcgat gaccccgcgg
      121 cggcggacga cccgttccag cactacctgg ccgacatgct gctggcgaag cgggagatgt
      181 ttcccgattt cgatgtgatc gcggcctcgg gcgttatcga tcccaattac tacctgatca
      241 acggctctga cgtgcatgag gcgcacttgg atccagttga acatttctgc cgctacggct
      301 ggcgggagtg gcgcaagccg aacatttatt tcgatatgca ctggtatctg cacaccaacc
      361 ctgtggtgga gcggatgaag atcaatccgg ccgtccacta tgtgcttgag ggcgaggcga
      421 tgggccggcg gcccgtaccg tatttcgatc cgctgtggta ccgtgagaca tacgcgatcg
      481 caccggaaca gaatgcgctg gcgcactacc tgacgcaccg gcggagccag aagttcagcc
      541 cgactcccat gttcaatgtg gcttggtatg tcgaccagca tcccgaggac gtgcgcggca
      601 accgcgatcc gttcgcgcat ttcctgcagg tgggcactta ccgcgatctc gatccatcgc
      661 cgacgttcaa tgcggcggag taccgcaagc gccatatcgg acgcccgagc cggaagttcc
      721 gccatctgat gcatcctgat cgggacaacc cgcttgtgca tttcctgcgc gcgggctacc
      781 gctgagcggg agcatcgggc gggttctctg tagggcttgc cggggcgaag cagattgctc
      841 cgctccgctc gcaatgacac aaatcagctc aacctgacct tcacataact accgggcgcg
      901 tcctcgatgg gtttcagctt gccgtcgccg gggacgcgcg cctgcacttg ctgcttggcg
      961 agcgcgctga cccagcggtg ccagtctggc caccaggatc cagccatctc tgtggcaccc
     1021 ttgaaccact cctcgggatc ggggggcaga tccttgttga tccagtggcc gtatttgccg
     1081 ctctccggcg gattgaccac gccggcaata tgtcccgacg ctgccagcac gaagcgcttt
     1141 ggtccgccca acaattgcgt gccgcgatag gtggatttcc atggcgcgat gtggtcctcg
     1201 cgcgtggaca ggaaatacgc cggcgtcttg atcttgcgca ggtcgatcgg cacgcccgcc
     1261 aggctgatgc cgcccggctt cgacagcaga ttttcctgat acattttgcg cagatagaag
     1321 ctgtgcatct tcgccggcat gcgcgtggag tccgcgttcc agtacagcag gtcgaacggg
     1381 aacgggtcgt tgcccagcag gtagttgttc acgacgaacg accagatcaa atcgttggcg
     1441 cgcagcatgt tgaatgtggt ggccatctcg gaaccttcta gatagccacg cttgttcatc
     1501 ttgccttcca gcgacttcaa ctgttcctca tcgatgaaca cgccgagttc gccggcatcc
     1561 tcgaaatcgg tcagtgtgac gaagaacgtc gccgacttta tgcggtcatc ttttttcgcg
     1621 gccatatagg caagcgtgct cgtcagcagc gtgccgccga ggcagtagcc gatggcgttg
     1681 acctcgcgct cgccggtcgc ctgctcgatc gcgccgagcg cgtcgaggta gccctgctgc
     1741 atgtagtctt cgaagttctt ctcagcgagt ttcgcgtccg ggttgaccca ggagatcatg
     1801 aacacagtgt ggccttgcga caccgcccaa cggacaaagc tgttccgcgg ccgcaggtcg
     1861 aggatgtaga acttgttgat ccacggcggc gcaatcagca gtggacgctt cagcaccttc
     1921 tcggtgctgg gtgtgtactg gatcaactgc atcaggtcgt tctggtagac caccttgccg
     1981 ggcgagacgc cgatgttctc gcccagcctg aaggcctcca tatcggtcat cttgatgcgg
     2041 agcttgccct tgccctgctc caggtcgctc agcaggttgt tcaggccctt cagcaggttc
     2101 tctccgccgg tctccgcggt cttgcgcagc acttccggat tggtgagcag aaagttcgat
     2161 gggctcatcg catcgacgaa ctggcgcgcg tagaaatcga ccttctgggc ggtcttcgcg
     2221 tccagaccgt cgacgtgcgt caccacgtcc tgcacatagc gcgcagacag cagatacgac
     2281 tgcttgatga aatcgaagat ctcattctcg cgccaggcat cgtccttgaa gcggcgatcc
     2341 gacgaaggcg catcgatcac cggcttggta tcggcaccga ggatgcggcg cgtggtgttc
     2401 tgccacagtg acatgtaatc gcgccaaaaa cctatctgtg cctcgaccag gtagctcggg
     2461 ttggcgatca ggcgcgcggt catctccatg aaggcgctgg cgatgttcag tggatcgggc
     2521 gagtggttct cgtccgcctg gcgcttcagc cactcgctca cgatgcgttg cgagcgttcg
     2581 gcgatgtcgg ccatggaacg accgatgtct gccgggtcgg gcagcttgaa cccggtttcg
     2641 gtcttctgcg tctcggccat acgcgtcctt tccgtgcgct ccaccacaag ctacaccagg
     2701 gttattgatt tggagcggat tcgacgatgc tcagtggcgt gaggggcagg caagttaggg
     2761 gggctggagg gcagcttcaa ggcagcgatg ggccggattc cagcgtgtct ggcctgcgtc
     2821 gttgcatggc gcccgccgcg ctcgtgctgc tgctcggggg gtgttcttcg ttcccatcgt
     2881 cgatgaaccc ggtgagctgg tggcacaacc tggaaggggg ggagatcgcc aaggagcgcc
     2941 cgccgccgcc gggtgccacg gagccttatc ccaacctggc cagcgtgccg ccgaagccag
     3001 aagcaccgga ccaggcgacg ctggaaaaca ttaccaacgc gctggtcgcc gaccgaacaa
     3061 atgcgcagca tcttgctgat gcagcgcctc tggcggaccc gtcgtcgccg agtgcctcgc
     3121 cggcgctgtt tgggaagggc acgttgcctc cgccgcctcc cgcgccgccg ccgggcactc
     3181 agaccacaag tgcgaacttg tccgccgccg aggcgccgcc ggccgccccc accaccccgg
     3241 cggcacccag tccgacaact cctgctgcaa agccttccgg gacggctgag gagatcgctc
     3301 cgccaccggc gaaggcgccc gtcggcgatg tgcagaccgc gcctctggct ccgcctggcg
     3361 cggccgcgca gccggctgcg cagagcgccg cggcgccgcc tctgcctacc acgcctccgc
     3421 cgcccccaag tttgccgggc gtcacggccg cggcgcgaac gccgcccgcc gcttcggctt
     3481 cggctcctgc gccaatgcca ccggtggccg cggtcgcgcc tcctcgtgcc gctccgccac
     3541 ccgttccgcc tgcacctgcg ccacagccgg cgcggaccgt gccgccgccc ccggcggcag
     3601 ctccaccatc cgcgccgacg acggttgcgt ctgcgacccc accacccgcg aaggtgccgg
     3661 ttccggcaag cgcgatcagc ctgacattca ttgccggttc ggctgatctg cccgcgttgg
     3721 cggcgggtca gctgaagcag ctcgccggac ggcgaggcaa tggcatcatt gccgttaccg
     3781 gttatggcga cgctgcatcc aacgatccgg acgcccaggc tgctgcactc acgcttggat
     3841 tgtcgcgcgc caaggctatg gccgcggcat tgacggcgga tggcgtgccg gcctctgccg
     3901 tgcagatgga cgccgaagcg attggtcgcg gtggcacggc gcgtctggta caataaccgt
     3961 ctgattcgag ggtctgtcca tgagttccga tccgtccggt gagttccacc gtattcgccg
     4021 cttgccaccc tatgtgtttg ccgaagtcaa cacggcgaag gctaaggcgc gcaccgccgg
     4081 cgacgacatc gtcgatctgg gtatgggcaa tccggacagc ccgacgccgc cgcatatcgt
     4141 tgctaagctc gtggaggccg tgcaggaccc gcggactcac aggtactcgg tgagcaaagg
     4201 gattccaggg ctgcggcgcg ccttggccgc atattatcag cggcggttcg atgtagggct
     4261 agacccggag accgaggtgg tggcgacgct cggttccaag gagggcctgg cgaacctggc
     4321 ggcggcgatc acatcacctg gcgacacaat cctggtgccg aacccgtcct atccgatcca
     4381 ccagttcggc ttcatcatcg cgggagccgc tgtgcggtca gtgcccgcgg cgccggatga
     4441 ggaaatgttg cgggcgctgg accgcgccgt gcggcactca gtaccaaagc cgacggcgct
     4501 catcgtcaac ttcccctcca atccgacggc gtatctcgcg gacctggatt tctaccgcga
     4561 ggtcgtcgct ttcgcgcggc ggcacgagat ctggatccta tccgatctgg cttacgcgga
     4621 gatctacttc ggcgacaacg cgccgccgtc gctgctgcag gtgccgggcg cgaaggagat
     4681 cgcggtcgag ttcaccagcc tgtcgaagac ctactcgatg ccgggctggc ggatggggtt
     4741 cgcggcgggc aatccgcggc tgatcaacgc gctgacgcgg atgaagtcgt acctggatta
     4801 tggtgcgttc acgccgattc aggtggcggc tgcgacagcg ctgaacgggc cgcaggattg
     4861 cgtggaccag atccggtcgt tgtatcggga gcggcgcgat gtgctgatcc gcgggttggc
     4921 ttcggccggc tgggatgtgc cgaacccgga tggctcgatg ttcgcctggg cgccgatacc
     4981 ggcgcgcttc gcgggtcttg ggagcgtgga attctccaag ctgctgctgg cacgcgccaa
     5041 ggtggcggtg tcaccaggcc tggggttcgg cgagcacggc gaggggtatg tgcggatcgc
     5101 gctggtggag aatacccacc ggctgcggca ggcggtgcgc aatataaggg cgttcctgca
     5161 ggcagggacg aaccaggcgg aggcagaggt ggttagcgtg gcgtcctgag ctcgttgccg
     5221 acgcggtgag cggatagctg aagttccagt gcatcgcctc cgttccgccc gcagttgatc
     5281 aagcgtgaag gggcagagcc gtctgcttgc gccgaccttc aattcgggtc tctctcttcc
     5341 agaagacctg tattgcttga tccagtctgc tatgcagccg tcactactcg tcaaggggac
     5401 gtcattcaca atgtccgcat gcttcgcttt gcagc
//
LOCUS       DATGEM010000608        10805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_183050, whole genome shotgun sequence.
ACCESSION   DATGEM010000608 DATGEM010000000
VERSION     DATGEM010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183050"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2600
                     /locus_tag="VKQ27_18745"
     CDS             <1..2600
                     /locus_tag="VKQ27_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY91029.1"
                     /translation="GYVGLLGAGDQLGCDALLQIALATGLYRETDLIYADEARVSPVS
                     HDHEPFFKPDYSPDLLLSTNYFGRPWFASTALLGRCGATTRELQENGEYDLALRCAER
                     ATYIHHVPKLLCLRGTQPIDDAEMESAALARAAARLGTPAEVRPTAIPGTWRLRRTRP
                     VSGKVSIIIPTCGAKGYIETCIKSLRERTAYPNIEIVCVDNIPDDQIAWKIWLQQNAD
                     KIAPTADAFNWSRFNNLGAEVATGEYLLFLNDDIEVVQPDWLDALLEHAQRPEVAVVG
                     PQLLYPDKKVQHAGMFLATPGTARHAFRFAAADEPGYFGLALTQRNVVAVTGACMLMR
                     RTVYEALGHFDEAHQIINNDLDFCLRAHESGKLIVYTPYATLLHYEAASRDGLKEVFD
                     LAQFEERWKIIFATGDPYFSARLTRHSDDYRPDDEPAEVIYAGHPMFRHDEIKRILVV
                     KVDHIGDFVTAIPAIRRLKQIFPSASIHVLAGRAARAFAEIEDCIDEFIEFEFFHAVS
                     GLGQKEISKEEYEDLRERLRPYRYDIAVDLRKHLDTRDVLRYTPARFLAGYDYMGQFP
                     FLDISLEWEGDRHLLRKRSHVTDDLINLVEAIGTAGTSDRTQLNLVALSSGVPEFLPD
                     HAKALFNLPVVAVHPGVGNVMRQWPAEHFASLVDLLTEKNGVNVVLIGGPEEKELAEQ
                     VQEHVIHRDAVVSLVGRTSLRQLPELLSACALYIGNNSGPKHIAAALGVPTIGIHSGV
                     VDAAEWGPIGARAVAVRRNMACSPCYLARTEDCPRAFACMRGLEPIAVQEVAETFLAR
                     PVARPTVTPLVEQVFVAPLPEPVARPEPAEASSPPKAARRRSAGAEQRAPRSSRRRRS
                     RQDATVSGD"
     gene            2646..3410
                     /gene="cysQ"
                     /locus_tag="VKQ27_18750"
     CDS             2646..3410
                     /gene="cysQ"
                     /locus_tag="VKQ27_18750"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HLY91030.1"
                     /translation="MDDDALISLAAALALEAGAAILMVRERGFDVMRKEDRSPVTEAD
                     HAAEAIIVAGLRKAVPDLPVIAEEEVSAGLITAAAASYWLVDPLDGTREFTAGNDEFA
                     VNIGLVRDGRPVLGVVAIPAAGELFGGIVGRGAWKRVGREQTAVHARLPPEEGLTVLA
                     SRYHGSSARLDEFLRDKRVAKLINFGSSVKFCRLAEGIADLYPRFGRTMEWDTCAPQA
                     VLEAAGGTVRTFDGAELRYGKPGWENPHFVCTGREP"
     gene            3594..4673
                     /locus_tag="VKQ27_18755"
     CDS             3594..4673
                     /locus_tag="VKQ27_18755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY91031.1"
                     /translation="MTVEFIGMIHHRQASEIHPPTGGVFDRGYVRDFAQAAEAAQFDR
                     VLVGYFSDGPDGFLLAAAAATHTERLGFLVAHRPGFVAPTLAARKFATLDQVTGGRAA
                     VHVISGGDDVDQFRDGDTLPKDERYARTDEYVHILKRIWTEDAPVDHTGRYYQFAGAS
                     TAMRCVQQPRIPIYFGGASDAAIEVAGKHADVYALWGETHEQVRELTTRVRTAAAKHG
                     REVRFSLSFRPILAPTEDAAWERAERIKEQIVELRAKAGLGPREPANEGSRRLLAAAA
                     QGERLDKRLWTGAALLTGARGNTTSLVGTPQQVAEAFCDYYDLGITTFLIRGFDPLED
                     CIEYGRSLLPLTRQMIAERQRRAAE"
     gene            4676..5827
                     /locus_tag="VKQ27_18760"
     CDS             4676..5827
                     /locus_tag="VKQ27_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLY91032.1"
                     /translation="MALLGNAVRHEWALDPDFLTVNHGSFGATPRVVLAAQQDWQRRM
                     EAQPSRFMRTVWPNALRHAADRIGAFIGADGNDIAFVENATTGCNAMLRSLRLQAGDE
                     IVVLTHGYNAVRNTVRYVCERAGARMVEATVPFPQPDADTIVANIAAALTPRTRLAVV
                     DHITSASALVLPLQRIVAACHDTGVPVLVDGAHGPAQVPLDMRALGADWYSGNCHKWL
                     CAPKGAAFLYTNASRQQDLHPTTISHGLGKGYLEEFDWTGTRDPSAWLATDVAIDFHR
                     RLGGEALMARNIALAANATALLARRLNTEVGASGALAGSMGVVRLPIPGAVTAEHSQA
                     LRTRLLAAGTDAPTHLHADAIWLRISAAAYNELEDYERLGEMVARVLRD"
     gene            complement(5876..6343)
                     /locus_tag="VKQ27_18765"
     CDS             complement(5876..6343)
                     /locus_tag="VKQ27_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLY91033.1"
                     /translation="MQFPQYEGEVLPQWIDANGHMNLAYYIVLFDGATDALYDLIGVG
                     QAYRDATGNSTFTAETHTLYERELLLGERVRVVSHLLGVDTKRLHYFHEMFHADTGHR
                     VAAQELIGLHIDLRIRRVAPFQPDLLARLRAAVRARGDQPLPQGVGRRISMPS"
     gene            6419..7795
                     /locus_tag="VKQ27_18770"
     CDS             6419..7795
                     /locus_tag="VKQ27_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91034.1"
                     /translation="MEAADGLAPPDRWRAMLAVSLAVAMSVLVTSLANIALPTIAHDM
                     HATPAASIWVVNAYQLAVTVTLLPFSSIGDIYGHRRVYVCGLAIYTAASLICAMAPSL
                     PVLVFGRVLQGFGGAGIMSVNGALVRFIYPRDSLGRGIGLNALVVAGSSAAGPSVAAA
                     IMSVASWPWLFALQVPGGALALLLSLRFLPRTPRIGHPFDPLSAVLNAITLGLFILGL
                     DGIGRGQGTITVLVQLSVSILIGWFFVHRQFSLAAPMLPVDLFRRPIFALSVATAICA
                     HAAQLIAFVALPFYFQYVSGLSPIQIGVLITPWPAALVVMAPIAGRLADRHSAGVLGG
                     LGLAVMTLGLLLVLSMPHQPSQPNVAWRLAVCGVGFGFFQSPNNRLMIASAPRDRAGA
                     GSGMLSTSRLVGQTTGSALVSLVFGITHGGSEGLELGTRLAIAMAASFAGIAMVLSIL
                     RVREPQSV"
     gene            complement(7800..>10805)
                     /locus_tag="VKQ27_18775"
     CDS             complement(7800..>10805)
                     /locus_tag="VKQ27_18775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY91035.1"
                     /translation="VELTSRLLRLQSALPPVGATFALSATGQLLAASSPFTAADADVG
                     NTAWFQRAISDNAMPLSLQRADSWLRPGPVLILSRVVHDQTGKTAGLVGALLRPEDVT
                     RLIGRTWLGPGVVVDLRGRDGTSLLPISAPEAPPAPADQDEWQWSRQLMSAANQMLGI
                     PQQLSVSEPLHTVDVAISAYMGTDRALKDHWLGTRALGILGAYLLAVWVTCIALAVSA
                     KSSGGGRAPAASPTGFGADWQIDLDKRGCIGAAHGYTPDRLREAAGRPLIAALGIASG
                     DPAAISIAEAFEARSRRDAIQVTLANDDGQERVHRLSIEPQANGQFVCVGRDITSDVR
                     LAAKLEAAMAESTTARDEAATVAHDRDRVLAALGHDVRTPMNSIMGICSLLLDSELEQ
                     EQRIWLERIRASCEALLAMLNGLLEIASGEVGGSGLQLDEVDVIGLVQEVTDTLQPQA
                     RDKGLELKTRYDDLLRGQWMVDPTRLRQVLFNLVGNAIKFTANGHVEVRASTVAAGDG
                     ASSIKIAVSDSGPGIAPEDRDRIFERFKRGREVESSGHAGLGLGLALCRENAVLMDGS
                     LTVESAIGVGSEFTFEFPAERIAGPVRNQRFAGRTALLVGDEDAKARTVANLLGDAGI
                     MVETAPDGYLGLALAERIEAQRGALDLVIVQGKLVGLAAEVFVLRLRDTPFGRRSVII
                     WLGEGGETAQVDAVVGAPGDPYQVASLAQQLLGQRSPFEVLEPMAPAARGGRILVVED
                     DKVNQSLLVAALARRGFSAFVANDGDEAVRLASHDSFDAILMDIQMPHRDGFEATRKI
                     RGMSSRVATIPIIALTGLKGPVMRKRCAEAGFTAVLEKPINLDRLGTTLRRWLPGGSA
                     VRDAVLPTGGQELARAEEYAADVSQVFLEEMVAVVGMERARACVDDFIADATARCMRL
                     GELLPGWEANAIVRNCEDISGLAETCGAIGLGEVLEEIADAVTRNDRSRAEVLVDRLE
                     RVTSRLGPAMAACLDDIARRWRDGANKAA"
BASE COUNT         1884 a         3421 c         3418 g         2082 t
ORIGIN      
        1 cgggatatgt gggattgctt ggcgcaggcg atcagcttgg ctgcgacgcg ctgttgcaga
       61 tagcgctggc taccgggctg tatcgcgaga ctgatctgat ctatgctgac gaagcacgcg
      121 tcagcccggt cagccacgat catgagccgt ttttcaagcc cgattattca ccggatctcc
      181 tgctatcgac caattatttc ggccggccat ggttcgcatc taccgcgctg ctcggccgtt
      241 gtggcgcaac aacccgcgaa ctgcaggaga atggtgaata cgatctcgct ttgcgctgcg
      301 ccgaacgtgc gacgtatatc catcatgttc cgaaattgct gtgcctgcgc ggcacacaac
      361 cgatcgacga cgccgagatg gaatctgctg cgctggcacg ggccgcagcg cgccttggca
      421 ccccggccga ggtgaggcct accgcgatac ccggcacatg gcgtctgcgg cggacgcgcc
      481 cggtaagcgg taaggtctcg atcatcatcc cgacctgcgg cgccaaaggc tacatagaga
      541 cctgcatcaa atcactgcgc gaacgcacag cctacccgaa catcgagatc gtctgtgtgg
      601 acaatattcc cgacgatcaa atcgcatgga aaatctggct acagcagaac gccgacaaga
      661 tcgcgccgac cgcggatgct ttcaactggt cgaggttcaa caatctaggg gcggaggtcg
      721 ccacaggcga atatctgctg ttcctgaatg acgatatcga ggtggttcag cccgactggc
      781 tcgatgcgtt gctcgagcat gcgcaaaggc cggaggtcgc cgtcgtcggc ccacaacttc
      841 tgtatcccga caaaaaggtg cagcacgctg gcatgttcct cgccacacct gggacagcgc
      901 ggcatgcatt ccgtttcgcc gccgcggacg aaccgggcta ttttggcctg gctctcaccc
      961 aacgcaatgt cgttgcggtg accggtgcct gcatgctcat gcggcgtacg gtgtacgaag
     1021 cgctcggcca cttcgacgaa gcgcatcaga tcatcaacaa tgatctggat ttctgcctgc
     1081 gggcgcatga gtcagggaag ctgattgtat atacgccgta tgcgaccctg ctgcattatg
     1141 aagcggcgag ccgtgacggg ctgaaggaag tgttcgatct cgcgcaattc gaggagcggt
     1201 ggaagataat attcgccacc ggcgatccat atttcagcgc gcggctcacc cgtcattcgg
     1261 atgactatcg gcccgatgac gagccagccg aggtgatcta cgccggtcat ccgatgttcc
     1321 gccacgacga aatcaagcga atacttgtgg taaaggttga tcacattggt gatttcgtca
     1381 ccgccattcc ggccattcgg cggttgaagc agatattccc aagtgcctcg atccatgtgc
     1441 tcgcaggccg tgctgcgcgg gccttcgcgg aaatcgagga ctgcatcgac gaattcatcg
     1501 aattcgagtt tttccacgcg gtgtccggtc ttggccagaa ggagatcagc aaggaggagt
     1561 acgaggatct gcgtgagcgc ctgcggcctt atcgttacga tattgccgta gatttgcgga
     1621 agcatctgga tacgcgcgat gtgcttcgct acacgccggc gcggttcctc gcgggctacg
     1681 actacatggg ccagtttccc ttcctcgaca tatcgctcga gtgggagggc gacaggcact
     1741 tgttgcgaaa acgcagtcac gtgacagacg acctgatcaa tctggtcgag gcgataggca
     1801 ctgccggaac gtccgatcgc acgcaactga atctggtggc cttgtcctcc ggcgtaccgg
     1861 aattcctgcc tgatcacgcg aaggcactgt tcaatctccc ggtggtggcg gtgcatcccg
     1921 gcgtcggcaa tgtcatgcgg caatggccgg ccgagcattt cgcctcgctg gtggacctgc
     1981 ttactgagaa gaacggcgtc aacgtagtgc tgattggtgg cccggaggaa aaggagcttg
     2041 cggaacaggt gcaggaacac gtgatacacc gggatgcggt ggtgtcgctg gttggccgca
     2101 cgtcactgcg tcagcttccc gagttgctca gtgcctgcgc cctgtacatc ggcaacaact
     2161 cgggaccgaa gcacattgct gcggcacttg gcgtgccgac catcggtatt cattcagggg
     2221 tagttgacgc ggccgaatgg ggtcctatcg gtgcccgtgc tgtcgccgtg cgccgcaata
     2281 tggcatgcag tccctgttac ttggcccgaa cggaggactg cccacgcgcc ttcgcatgca
     2341 tgcgcggctt ggaaccgatc gccgtacagg aggtcgcgga gacattcctt gcgcgacccg
     2401 tggcgcgtcc tacggtcacg ccgctggtag agcaagtgtt cgtcgcgccg ttgccagagc
     2461 cggtggctcg accggaaccg gctgaagcat ccagcccgcc caaggctgcc cggcggcgca
     2521 gcgccggagc cgaacagcgc gcgccaaggt cgtcgcgccg caggagatcc cgccaggacg
     2581 ccactgtctc tggcgactag ctgctttgcg ttgccatcag ggcaacccgt cagtatgacc
     2641 tgtgcatgga cgacgatgca ttgatttcac tcgccgcagc actcgccctg gaggcaggtg
     2701 ctgccatcct gatggtgcgc gagcgcggtt tcgacgtgat gcgaaaggag gaccgttcgc
     2761 ctgtcactga ggcagatcat gcggcggaag ccatcatcgt cgccgggctg cgcaaagctg
     2821 tgccggatct gccggtgatt gccgaggagg aagtgtctgc aggccttatc accgcggcgg
     2881 ccgcgtcata ctggctggtt gatccgctgg atggtacgcg ggaattcacc gctggcaatg
     2941 acgagtttgc agtgaacatc ggtctggtgc gcgacggaag accggtccta ggcgtagttg
     3001 cgatcccggc ggcgggtgaa ttgttcggcg gcatcgtcgg gcgtggggcg tggaaacgtg
     3061 tgggcagaga gcagaccgcg gttcacgcgc ggttgccgcc tgaggaaggg ctgaccgtac
     3121 tcgccagtcg ctatcacggc agcagtgcaa ggctggatga gttcctacgc gataagcgcg
     3181 ttgccaagct gatcaacttc ggttccagcg tgaaattctg ccgcctggcg gaagggatcg
     3241 ccgatctcta tccacgcttt ggccgcacga tggagtggga cacttgcgcg ccacaggcag
     3301 tgctggaagc cgctggtggg acggtccgca ccttcgatgg tgctgaactg cgctacggca
     3361 agccgggctg ggagaacccg cacttcgtct gcaccggacg ggagccataa gtcagcagct
     3421 tgctgcgttc aggcgagtga cgacaacaac agcagcgcaa cgatcgacgc caaccgcatc
     3481 accccccgct atattgttgc aattgttgca cgattgtgcg cggttgcggt cagttggcga
     3541 gacgtgcaca actggcgttg ttatgttcgg cgatgttcag cggagccttc gcgatgacgg
     3601 tcgaattcat tggcatgatc catcatcgtc aggcgtcaga gatacatccg ccaactggtg
     3661 gtgtgttcga tcgaggctac gtgcgtgact tcgcgcaggc ggccgaggcg gcccagttcg
     3721 atcgcgtgct ggtcggatat ttctccgatg gtcctgatgg atttctgctc gctgcggctg
     3781 cggccacgca caccgagcgg cttggtttcc ttgtcgcgca ccggcctggc ttcgttgcgc
     3841 ctacgctggc agcgcgcaag ttcgccacac tggatcaggt gaccggcggg cgcgcggccg
     3901 tgcatgtgat ctccggcggc gacgatgtcg atcagttccg cgacggcgat acactgccga
     3961 aggacgaacg ctacgcgcgc accgatgagt acgtgcacat cctgaagcgt atctggaccg
     4021 aggacgcgcc ggtggaccac acaggtcgtt actaccagtt tgccggcgcg tcgactgcga
     4081 tgcgctgcgt acagcagccg cgcatcccga tctatttcgg cggtgcctct gatgctgcca
     4141 tcgaggtggc gggcaagcat gccgatgtct atgcgctctg gggtgaaacg cacgagcagg
     4201 tccgtgagct tacgacgcgt gtacgcactg cggccgcaaa acatgggcgc gaggtgcgct
     4261 tcagcctgtc gttccgtccg atcctggcgc ccaccgagga tgcggcatgg gagcgggctg
     4321 aacgtatcaa ggagcagatc gtggaactgc gggcgaaggc aggacttgga ccccgcgagc
     4381 ccgccaatga aggctcgcgc cgtctgcttg ccgccgccgc gcagggcgag cggttggata
     4441 agcgcctgtg gaccggcgcc gcgctgctga ccggcgcacg cggcaacacc acaagcctgg
     4501 tgggaacgcc gcaacaagtg gccgaggcgt tttgcgacta ctacgatctc ggcatcacca
     4561 ccttcctgat ccgcggcttc gatccgctgg aggattgcat cgagtacggc cgcagcctgc
     4621 tgccgttgac cagacagatg atcgctgaac gccagcggcg tgcggcggaa tagccatggc
     4681 gctgctgggg aatgcggtgc gccatgagtg ggcgctcgat ccggattttc tgaccgtcaa
     4741 ccacgggtcg tttggcgcga cgccgcgggt agtgcttgcg gctcaacagg actggcagcg
     4801 gcgcatggag gcgcagccaa gccgatttat gcggacggta tggcccaatg cgctgcgcca
     4861 tgccgctgac cggatcggcg cgttcatcgg cgcggacggc aacgacatcg ccttcgtcga
     4921 gaacgccacc acaggctgca atgcgatgct gcggtcgctt cgtctgcaag ccggtgatga
     4981 gatcgtggtg ctgacccacg ggtacaatgc agtgcgtaac acggtgcgct acgtgtgcga
     5041 acgtgccggc gcgcgcatgg tggaagccac ggtgccgttt ccccaaccgg acgccgatac
     5101 gatcgttgcg aacattgctg ctgcactaac gccgcgtacg cgccttgcag tggtggatca
     5161 catcacatcg gccagcgcgc tggttctgcc gctgcagcgc atcgtcgcgg cctgccatga
     5221 tactggcgtg ccagtgctgg tcgatggtgc gcatgggccg gcacaggtgc cgctggacat
     5281 gcgcgcgctc ggtgccgact ggtatagcgg caattgccat aagtggctgt gtgcgccgaa
     5341 gggggcggca ttcctgtata caaacgcgag ccggcaacag gatctgcatc cgaccacgat
     5401 ctcgcatggt ttgggcaagg gctatctgga agagttcgac tggaccggca cgcgcgatcc
     5461 cagcgcgtgg ctcgccaccg atgtcgcgat cgacttccac cggcgtctcg gtggcgaggc
     5521 attgatggcg cgtaacatcg ccctcgccgc gaacgcaacg gcgttgctgg cccgccggct
     5581 caacaccgag gtgggtgcga gcggagcgtt ggcgggcagt atgggcgtcg ttcgcttgcc
     5641 gatcccgggt gccgtaactg ctgaacattc gcaggcactg cgcacgcgtc tgcttgccgc
     5701 cggcaccgac gcgccgactc atcttcacgc cgatgcgatc tggctgcgca tctcggccgc
     5761 cgcgtacaac gaactcgagg attacgagcg tcttggcgag atggtcgccc gcgtgctgcg
     5821 ggattaagcg gctgacgcct caatatgagc gaggggtgtt gtcgccacac tcaaatcagg
     5881 aaggcatcga aatccgccga cccacgcctt gcggcaaagg ctgatcgccg cgcgctcgaa
     5941 cagccgcccg caatcgagcc agcaggtctg gctggaatgg cgccacgcgc cttatccgaa
     6001 gatcgatatg caggccgatc agttcttgcg ccgcgacgcg atggccagtg tcggcgtgga
     6061 acatctcgtg gaaataatgc agccgcttcg tatccacacc gagcagatgc gacacgacgc
     6121 gtacccgctc gcccaacagc agttcgcgct catacagcgt gtgcgtctcg gcagtgaagg
     6181 tcgagttgcc ggttgcgtcg cgatacgcct ggccgacgcc tatcaggtcg tacagtgcgt
     6241 cggtcgcccc gtcgaaaagc acaatgtagt aagctaggtt catgtggcca ttggcatcga
     6301 tccactgcgg caggacctcg ccctcgtatt ggggaaactg catggtcagg cagattgttg
     6361 acgcagtggc acatggtcaa gcacacaggc cgttgtgcga cagatacgta tcagggacat
     6421 ggaggcggcc gatggcctgg cgccgccgga ccgctggcga gcgatgcttg cggtgtcgtt
     6481 ggcggttgcc atgtcggtgc tggttacgtc gctggcgaac attgcactgc ccaccatcgc
     6541 ccacgacatg cacgcaacgc cagccgcctc gatctgggtg gtgaacgcgt accaacttgc
     6601 ggtcactgtc acgctgctac cgttctcgtc gattggggac atttacggcc atcgtcgcgt
     6661 ctacgtctgc ggtctggcga tatacacggc tgcatcgctg atatgcgcga tggcgccgtc
     6721 gctcccggtg ctggtgtttg gtcgcgtgct gcagggtttt ggtggcgcag ggatcatgag
     6781 tgtcaacggc gcgctggtcc gcttcattta tccgcgcgat tcgcttggcc gcggcatcgg
     6841 cctgaatgca cttgtggttg ccggctcctc cgctgccggc ccatcggtgg cggctgcgat
     6901 catgtcggtt gcctcctggc cctggctgtt cgccttgcag gttcctggcg gggcgctcgc
     6961 gctattgctg tcgcttcgtt tcctgccacg aacgccgcgc atcgggcatc cattcgatcc
     7021 gctcagcgcg gtgctgaatg cgatcacact ggggctgttc atcctcgggc ttgatggcat
     7081 cggtcgcggc cagggaacca tcactgttct ggtgcaactc tcggtcagca tcttgattgg
     7141 ctggttcttc gtgcatcggc agttttcgct tgccgcgccg atgctgccag tcgatctgtt
     7201 ccgccgcccc atcttcgcgt tgtccgtggc caccgcgatc tgcgcacacg ccgcacagct
     7261 catcgcattt gtcgcgctgc cattctattt ccagtacgtc agcgggctct caccgataca
     7321 gattggcgtg ttgatcacgc catggccggc agccctcgtg gtcatggcgc cgattgccgg
     7381 ccgtctcgcc gatcgtcatt cggcaggggt gctaggaggc ctggggctcg cggtgatgac
     7441 actgggattg cttctggtgc tgtccatgcc gcatcagcca tcgcagccga acgtagcctg
     7501 gcgattggcc gtttgcggcg tgggctttgg cttcttccag tcgccaaaca accggctgat
     7561 gattgccagc gcgccccgtg accgcgcggg tgcaggtagc ggcatgttgt caacgtcacg
     7621 ccttgtcggt cagacaaccg gcagtgcgct ggtatctctg gtgttcggga tcacgcacgg
     7681 tggcagcgag gggctcgaac tcggtaccag gctcgccata gcaatggcag ccagctttgc
     7741 cggcattgca atggtgttga gcatattgcg cgtacgcgag ccgcagtcag tctgacccct
     7801 cacgccgcct tgttggcgcc gtctctccac cgccgcgcaa tatcgtccag gcacgctgcc
     7861 attgccggtc cgagccggga tgtcacccgc tcgagcctat ccaccagcac ttcggcacgc
     7921 gagcggtcgt tgcgcgtcac cgcgtcggca atctcttcca acacttcgcc cagccctatc
     7981 gcgccacagg tctcggccag gccgctgatg tcctcgcagt tacgtacgat cgcgttcgcc
     8041 tcccagcccg gcagtaactc gccgaggcgc atgcagcgcg cggtcgcgtc ggctatgaaa
     8101 tcgtcgacgc atgcccgcgc gcgctccatc ccgaccacag ccaccatctc ttcgaggaat
     8161 acctgcgata cgtcggcagc gtactcttct gctcgcgcca actcctgtcc gcccgttggc
     8221 agtaccgcgt cgcgtactgc actgccgcca ggcagccacc ggcgcagggt ggttcccagc
     8281 cgatcgaggt taattggctt ttccagcacc gcggtgaacc cggcctcggc acagcgtttg
     8341 cgcatcactg ggcccttgag gccggtcagc gcaatgatcg ggattgtcgc cacccgactg
     8401 ctcatgccgc ggatcttccg cgtagcctcg aacccgtcgc gatgcggcat ctggatgtcc
     8461 atcaggatgg cgtcgaagct gtcgtggctg gcaagccgca cggcctcgtc tccgtcgttg
     8521 gcgacgaacg cggaaaagcc gcgacgcgcc agtgcggcga cgagcagtga ctggttcacc
     8581 ttgtcgtctt ccaccaccag aatgcggccg ccgcgtgctg ccggcgccat cggctccaag
     8641 acctcgaacg gtgaacgctg tcccaggagc tgctgggcta gggatgccac ttgatacgga
     8701 tcacctggtg cgccgacgac cgcgtcgact tgcgccgttt cgccgccctc gccgagccag
     8761 atgatcacgg atcgccggcc aaacggggta tcacgcaggc gaagaacaaa cacctcggct
     8821 gccaatccga ccagcttgcc ttgcacgatg acgaggtcca gggcaccgcg ctgtgcctcg
     8881 atgcgctcgg ccagcgccag ccccagataa ccatccggcg ccgtctctac cattatgccg
     8941 gcatcgccga gcagattggc gacagtacgg gccttagcgt cctcgtcacc caccagcagc
     9001 gcggtgcgtc cggcgaatcg ttggttgcgt acggggccgg cgatccgttc ggcggggaac
     9061 tcgaaggtga attcgctgcc gacacctata gcgctttcca cagtcagcga gccgtccatc
     9121 aggacagcgt tttcgcggca cagcgccaaa ccgagtccca agcccgcatg ccctgaactc
     9181 tcgacctcac ggccgcgctt gaaccgctca aagatccggt cgcggtcctc cggcgcgatg
     9241 ccaggtccgc tgtccgacac ggcgatcttg atcgatgaag cgccatcccc ggcggccacc
     9301 gtggacgccc ggacctcgac atgtccgttc gcagtgaact tgatcgcgtt gccgacgagg
     9361 ttgaacagca cctgccgcag ccgcgtcgga tcgaccatcc actggccgcg cagcaaatcg
     9421 tcgtaccgtg tcttcagctc cagaccctta tcacgcgcct gtggctgcag cgtgtcggtc
     9481 acttcctgca ccagtccgat gacatcaact tcgtcgagtt gaagcccaga gccgccaacc
     9541 tcaccgctgg cgatctccag taggccgttc agcatggcca gcagggcctc gcagctcgcg
     9601 cggatccgct cgagccagat gcgctgttct tgctctagct cactgtccag cagcaaggag
     9661 cagatgccca tgatggagtt cattggcgtg cgcacgtcgt gtcccaaagc ggccagcacc
     9721 cggtcgcggt cgtgtgcgac ggtggcagcc tcatctcgtg ccgtcgtgct ctcggccatc
     9781 gcggcttcca gcttcgccgc cagccgtacg tccgacgtaa tgtcgcgacc gacgcatacg
     9841 aactgcccgt tcgcctgcgg ctcgatgctg agcctatgaa cgcgctcctg gccatcatcg
     9901 ttggcaaggg tgacctggat ggcatcccgg cgcgatcgcg cctcgaatgc ttcggcgata
     9961 ctgattgctg ccggatcgcc ggacgcgatc ccgagtgcag cgattagtgg cctgccagcc
    10021 gcctcgcgga gccggtctgg cgtatagccg tgggccgcgc caatgcagcc acgcttgtcc
    10081 aggtcgatct gccagtccgc gccgaagccg gtaggtgacg cggcaggtgc ccgtcctccg
    10141 ccgctcgact tcgccgacac ggcgagtgca atgcaggtca cccagaccgc cagcaggtag
    10201 gcgccgagga tgccgagtgc ccgcgtaccc agccaatgat ccttcagggc gcggtcggtg
    10261 cccatgtacg cgctgatcgc cacatccacg gtgtgcaatg gctcggaaac tgacaattgc
    10321 tgcgggatgc ccaacatctg gttcgcggcc gacatgagct gccggctcca ttgccactcg
    10381 tcctggtccg ccggcgctgg cggagcctcc ggcgcagaaa ttggcaggag tgaggtgcca
    10441 tcccgtccgc gaaggtcgac cacgacgccc ggtccgagcc acgtccgccc gatcagtcgc
    10501 gtgacatcct ccggtcttag cagagctccg accaggccag ccgttttgcc tgtctggtcg
    10561 tgcacaaccc ggctcaatat cagcactggc cctggccgca gccacgagtc cgcccgctgg
    10621 agcgaaagcg gcatcgcgtt gtcagagata gcgcgctgga accaagccgt gtttcccacg
    10681 tccgcatctg cggcggtgaa cggggaggac gccgcaagca actggccggt tgcgctcagt
    10741 gcgaaagttg cccccaccgg tggcagcgcg ctctgcaacc gcagcagccg ggacgtcagt
    10801 tccac
//
LOCUS       DATGEM010000609         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1295335, whole genome shotgun sequence.
ACCESSION   DATGEM010000609 DATGEM010000000
VERSION     DATGEM010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295335"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1647)
                     /locus_tag="VKQ27_18780"
     CDS             complement(409..1647)
                     /locus_tag="VKQ27_18780"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HLY91036.1"
                     /translation="MPDRRRLGAANRQNANSCSGRRADPRRGAGVLLKLARRFSVAGL
                     ALAAALLPQAALAHAQLVQSDPAPNAVLNSVPGSITLLFTEQVTPAGAGIKVFSPSGN
                     QVALPAVAAGSVLVAALHSTEPGSYVVTWQVFAADTHPSRGAFGFEVGHSTTNPYSGL
                     LNATQLGTTSPLGVALQAFGRWVHFVGFSLVFGVVAYQVAMRRKERLKRLIGAGVVLL
                     ITAEPIGLLAQLASLSFDGDTAVAILSSGFGRLLALRLGAALLVWVVLSLESPWPVLG
                     IGAVMAALDGASAHAVPGFAGVGAALTAAHVIGLGLWVGGVAAYVFDQRRTDALGFGR
                     YAAITFSVAAISGLVLAVVHTNLFTALANDYAYVLMLKVLIVGGALAAVLLRRHRVEL
                     GVMAAILGVATILAALPPPS"
     gene            complement(1548..>2284)
                     /locus_tag="VKQ27_18785"
     CDS             complement(1548..>2284)
                     /locus_tag="VKQ27_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91037.1"
                     /translation="VFIRNRPADMGLRAYGATEADDVPLATGSPIRNAFRGLGIGVRS
                     RDFWLLGGSFFICGASTNGLIGTHLIPASMDHGMAEVTAASLLAVIGVFDVVGTIASG
                     YLTDRFDSRWLLFMYYGLRGLSLLLLPYVFGSPAFGLILFIVFYGLDWVATVPPTVQL
                     ARKIFGQQNFAIVYGWIFASHQLGAAAIAAAAGAARTYFGDYQIAFMTAGLLCLIAAG
                     LVLRIGKTQTAAPVDVRIPVEAPAYS"
BASE COUNT          483 a          738 c          731 g          332 t
ORIGIN      
        1 aaatatgtga ataaagaacc atggcgccgc cactataccc ctggggcgca ctccattcag
       61 gttgctgccg gttctgttag ccggcgttcg aggaagcgct gttcggcacc gtcgccggtg
      121 agttccctcg caggtcgata agcgtcgcgc gactagtcgg atcagtgact gcgggtggtc
      181 ctagggcgct gtaagagggc aaaacgcgac ccatgcccga gcctgcgcaa tcgcgtgccg
      241 cggccgcgca tcagaagcct cgccgcctcg aagaggtgca gcacgtgctc cgcgcacgtt
      301 gcagcccaca aggcgagaag acgatgatcc gagtccgtga gcgtaccccc gcgacggatg
      361 gtgatggatc tcggatcgcg ccgtttcggc aggatcacgt ttcctgagct aactcggtgg
      421 cggcagcgcc gcgaggatgg tggccactcc aaggatggca gccatcacgc cgagttcgac
      481 ccgatgccgg cgcagcagga cagcggccaa tgcgccgccg acgatcagca ccttgagcat
      541 caaaacgtat gcgtagtcgt ttgcaagcgc ggtaaacagg ttcgtgtgca ccaccgccaa
      601 cacgagcccg ctgatcgccg cgactgagaa cgtgatcgcc gcgtaacgtc cgaatccgag
      661 ggcgtctgta cgcctttgat caaacacgta ggcggccacg cctccgaccc acagtcccaa
      721 accgatcacg tgcgcagccg tgagcgcagc gccaacgccg gcgaaccccg gcacagcgtg
      781 cgcacttgcg ccatccagcg ccgccatcac agctccgatt ccgagcaccg gccacggtga
      841 ctcaagcgac agcacaaccc aaacaagcag tgccgcgcca aggcgcaatg ccaggaggcg
      901 gccgaaccct gacgatagga tcgccaccgc ggtatcccca tcaaacgaga ggcttgccaa
      961 ctgggcaagc agaccaatcg gctcggccgt gatcaacagg accacgccgg cgccgatcaa
     1021 tcgcttcaat cgctcctttc gccgcattgc cacctggtac gcgacgacac caaacacaag
     1081 cgagaagccg acgaagtgca cccagcgccc gaacgcctgc agcgcaacgc cgagcggcga
     1141 tgtggtgccc agctgcgtcg cattcagcag accggagtac gggttggtgg tcgagtgccc
     1201 gacctcgaag ccgaatgcgc ctcgcgatgg gtgggtgtcc gcggcgaaga cctgccaggt
     1261 gaccacgtat gacccaggct cggtggagtg gagtgccgcc accaacaccg atccagccgc
     1321 gaccgctggc aatgccacct ggttccccga tgggctgaac actttgatgc ccgctccggc
     1381 gggcgtgacc tgctcggtga agagcaatgt gatcgaccct gggaccgaat tcagcaccgc
     1441 gtttggcgcg gggtccgact gaaccagctg agcgtgagcg agagcggctt gagggaggag
     1501 cgcagcagcc agggccaggc cggcgacaga aaaacgccga gccagcttca ggagtacgcc
     1561 ggcgcctcga cggggatccg cacgtcgacc ggagcagctg tttgcgtttt gccgattcgc
     1621 agcaccaagc cggcggcgat caggcacagg agtccggcgg tcatgaaagc gatctgatag
     1681 tcgccgaaat aggtccgcgc cgctcctgcg gcagcagcga tcgcggctgc gccaagctgg
     1741 tgggaggcga agatccatcc gtacacgatg gcgaaattct gctgtccgaa tatctttcgc
     1801 gccagctgaa cggttggcgg aaccgtcgcc acccagtcga gcccgtagaa cacgatgaaa
     1861 aggatcagac cgaacgcggg gctgccgaac acatagggaa gcagcagtag cgacagtccg
     1921 cgaaggccgt agtacatgaa aaggagccag cggctgtcga atcggtccgt gaggtatccc
     1981 gacgcaatgg tgcccaccac gtcgaagacg ccgatcaccg caagcaagct ggcggccgtg
     2041 acttccgcca tgccgtgatc catcgatgcg ggtatcagat gcgtcccaat gagaccgttg
     2101 gtggacgccc cgcagatgaa gaagctgccg ccgaggagcc agaagtcgcg cgagcgcacg
     2161 ccgatgccca gcccgcggaa ggcatttcga atcgggctgc ccgtggcgag cgggacatcg
     2221 tcggcctcgg tggcgccgta agcccgcagc cccatgtcgg cgggtcggtt gcggatgaaa
     2281 accg
//
LOCUS       DATGEM010000610         5302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1560305, whole genome shotgun sequence.
ACCESSION   DATGEM010000610 DATGEM010000000
VERSION     DATGEM010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560305"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VKQ27_18790"
     CDS             <1..1082
                     /locus_tag="VKQ27_18790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLY91038.1"
                     /translation="TARFVADHLRAFGLEVHEGIGGTGVVAVLRRGTSTRSIGLRAEL
                     DALPITERSGKPHASRNVGAMHACGHDGHTASLLGAARLLSEQTGFDGTVHFVFQPAE
                     ENIGGGIRMIEDGLFQRFPMEAVYAVHNWPGLPVGQIACRTGAMMAAVDNFELRFTGT
                     GAHAAMPHLGDDPILAAGAFINSVQRIVSRAVDPQTPLVVSLTQVHGGNVGNVIPGEV
                     WLQGTCRFFDASLSDLCERQLSQIAAGIASAHGVRAVLDYQGGYPPVINTADPVANAV
                     AAAQSTVGSDDVATDFAPSLGCEDFAYMVRTAGGAYAWVGAGEAGPGEGLHGARYDFN
                     DAIVPVVLRYWVNLVCQALPARASS"
     gene            complement(1102..1764)
                     /locus_tag="VKQ27_18795"
     CDS             complement(1102..1764)
                     /locus_tag="VKQ27_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91039.1"
                     /translation="MRSLVRAGLAALAITGLCSATAHAQFRTYYHTGAWDAFSGQGSD
                     GKTVCGVGTTNPADNRSVSIRFLIGGEDVLFEVKKPTWSIPAGTKLVVMIQVGANVPW
                     TEQAQGDGQTISWTLDRGTMQAFDQQFRAAGSMTLSFPTGNEPPWTMSLTGSMAISNA
                     FGRCVTDLTAQQSPPPAPAAPTQPFGQTPTQPFAPAPGQPAPASDGTQPTQPAPSPPP
                     PR"
     gene            1887..3092
                     /gene="pcaF"
                     /locus_tag="VKQ27_18800"
     CDS             1887..3092
                     /gene="pcaF"
                     /locus_tag="VKQ27_18800"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HLY91040.1"
                     /translation="MADAYICDFVRTPIGRYGGALKDVRTDDLAAHPMRVLKERNVSV
                     DWEQLDDVIYGCANQAGEDNRNVARMALLLAGLPAKIPGATINRLCGSGLDAVGTAAR
                     AIRAGDAELMIAGGVESMTRAPFVMGKATEAFSRSAEIYDTTIGWRFVNPLLKAQYGV
                     DAMPETAENVAEDFQVMRADQDAFAYRSQMRAKAAQDSGFFAREIAPVSIEGRKGTVT
                     VDRDEHPRGDTTLEGLAKLRAPFRKQGGTVTAGNASGVNDGAAALIIASEAAAKRHGL
                     TPRARVVAMVTAGVAPRIMGIGPAPATEKLLDRLGMKVGDIDVIELNEAFAAQGLAVT
                     RQLGLPDDAEHVNPHGGAIALGHPLGMSGARLALTALNALETRGQKRAVATMCIGVGQ
                     GIAALIERV"
     gene            3220..4497
                     /gene="eno"
                     /locus_tag="VKQ27_18805"
     CDS             3220..4497
                     /gene="eno"
                     /locus_tag="VKQ27_18805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLY91041.1"
                     /translation="MAAIADIIAREVLDSRGNPTVEVDVVLDSGAMGRAIVPSGASTG
                     AHEAVELRDGDKSRFGGKGVQTAIANVEGEIFDAIGGMDPIEQVKIDEIMIDLDGTAN
                     KSRLGANAILAVSLATAKASAAELGLPIYRYVGGVYARTLPVPMMNIVNGGVHADNPI
                     DIQEFMIQPIGAGTVADGIRMGSEIFASLKKGLHDAGHNTNVGDEGGFAPNLKSADEA
                     LGFIAKACETAGYRPGEDVTFALDPASSEFFKDGKYALEGEGKTLDADGIVKYYEALI
                     SRYPIVSIEDGCAEDDWEGWKHLTATLGNKLNIVGDDIFVTNPERLRRGIKEHSANAI
                     LIKVNQIGTLSETLETMEIAHNAGFRCVMSHRSGETEDATIADLAVATNCGQIKTGSL
                     SRSDRTAKYNQLMRIEAELGPAARYAGRTILRR"
     gene            4767..>5302
                     /locus_tag="VKQ27_18810"
     CDS             4767..>5302
                     /locus_tag="VKQ27_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLY91042.1"
                     /translation="MLSLADGARAQNELKSPTMDAIKQRGQLICGIDTGIPGYAFQDS
                     KGVWQGLDVSYCRAIADALLGSPDKVKYVGTTSKVRFSVLASGEIDVLIRDSEQTFVR
                     NTQLGLDEPAYNFFTAQTFMVRKSLGVAHAKDLNGATICLLTGTTLETNIADFNRANN
                     IKINTLLFDKPEEAFAAA"
BASE COUNT          955 a         1701 c         1739 g          907 t
ORIGIN      
        1 ccaccgcgcg ttttgtcgcc gaccacctgc gcgctttcgg tctcgaggtg cacgaaggga
       61 ttgggggcac cggcgtcgta gccgtcctcc ggcgcggcac gagcacccgg tccatcggct
      121 tacgcgcgga actcgacgcg ctgccgatca cggagcgaag cggcaagccg catgcatcgc
      181 gcaatgttgg cgcgatgcat gcctgcgggc acgacggaca cacggcgtcg ctgctcggcg
      241 ccgcgagact gctgagcgag cagacaggct tcgacggcac tgtccacttc gtctttcagc
      301 cggctgagga aaacatcggt ggcggcatcc gcatgatcga ggatgggctg ttccagcgtt
      361 tccccatgga agccgtctac gccgtgcaca attggccagg cctgccggtc ggccagatcg
      421 cctgccgcac cggcgcgatg atggccgcgg tcgacaattt cgagctccgg ttcaccggca
      481 cgggtgccca cgccgcgatg ccgcatctcg gcgacgatcc gatcctcgcc gctggcgcgt
      541 tcatcaactc ggtgcagcga atcgtcagcc gcgcggtcga tccgcaaacg ccgctcgtcg
      601 tcagcctgac ccaggtccat ggcggcaacg tcggcaacgt gattcccggc gaagtctggc
      661 tgcaaggcac ctgtcgcttc ttcgatgctt cgctgtcgga tctgtgcgaa cggcagctca
      721 gtcagatcgc cgcaggtatc gcatctgcgc acggtgtccg cgcggtgctc gattatcaag
      781 gcggctatcc gccggtgatc aacactgccg accccgtggc caatgccgtg gcggcagcgc
      841 aatctacagt gggtagcgat gatgtcgcca cggacttcgc cccgagcctc ggctgcgagg
      901 acttcgccta tatggtccgc acagccggag gcgcctatgc ctgggtcggt gccggcgagg
      961 ccggtcccgg cgaaggcctg cacggcgccc gctatgattt caacgacgcg atcgtgcctg
     1021 tcgtgctgcg ctactgggtg aaccttgtct gtcaggcact gcccgcacgc gcgagcagtt
     1081 gacgaggttt gacgaggcct gtcaccgcgg cggcggtggg ctcggtgccg gctgcgtcgg
     1141 ctgcgttccg tcggaggcag gcgccggctg tccgggcgct ggcgcgaagg gctgcgtcgg
     1201 tgtctgcccg aacggttgcg tcggcgcagc cggcgcaggt ggcggggact gctgcgccgt
     1261 caggtcggtc acacaccgtc cgaatgcgtt cgaaatcgcc atcgagccag ttaggctcat
     1321 tgtccacggc ggctcatttc ccgtgggaaa actcagcgtc atcgagcctg cggccctgaa
     1381 ctgctgatcg aaagcctgca tcgtgccccg gtccagcgtc cacgaaatcg tttggccatc
     1441 accctgggcc tgctccgtcc acggcacatt cgccccaacc tgaatcatca ccaccagctt
     1501 ggtccccgcc ggtatcgacc aggttggctt cttcacctcg aacaacacat cctcgccgcc
     1561 gatcaggaac cgtatcgaca cgctgcggtt atctgcaggg ttggtcgtcc cgaccccgca
     1621 aaccgtcttg ccatcgctgc cttgcccgct gaacgcgtcc caggcgcccg tgtgatagta
     1681 ggtgcgaaac tgcgcgtgcg ccgtagcact gcacagtccg gtgatcgcca gcgcggcaag
     1741 tccagcgcgg accagggatc gcatgtcgtt accctcctga tgcgaggttc tctcgctgcc
     1801 atgctcaggc ggcataattg cggcaacttg ccggaggccg ccggatgccc taggccagcg
     1861 tcaatcgaat gttcgggagg atcgccatgg ccgacgccta tatctgcgac ttcgtccgca
     1921 ccccgatcgg ccgctacggc ggcgcactga aggacgtgcg caccgacgat ctcgccgccc
     1981 acccgatgcg cgtgctgaag gagcgcaacg tgtcggtcga ttgggagcaa ctcgacgacg
     2041 ttatttacgg ttgcgctaat caggcaggcg aggacaaccg caacgtcgca cgcatggcgc
     2101 tgctgttggc aggactgccc gccaagatcc ccggtgccac gatcaaccgc ctctgcggct
     2161 ccggcctcga cgccgtgggc actgccgccc gcgccatccg tgccggcgat gcggaactga
     2221 tgatagccgg tggcgtcgag agcatgaccc gcgcgccatt cgtcatgggg aaggcgacgg
     2281 aggcgttctc gcgttcggcg gagatctacg acaccaccat cggctggcgc ttcgtcaacc
     2341 cgctgctgaa ggcccagtac ggcgtcgacg cgatgccgga aacagcggag aacgtcgccg
     2401 aggatttcca ggttatgcgc gcggatcagg acgccttcgc ctaccgctcg cagatgcgtg
     2461 ccaaggcggc gcaggacagc ggcttcttcg cgcgcgagat cgcgccggtg tcgatcgagg
     2521 gtcgcaaggg taccgtcacc gtggaccgcg acgagcatcc gcgtggcgac accactctgg
     2581 agggccttgc caaactgagg gcgccgttcc gcaaacaggg cggtactgtc acggcgggca
     2641 atgcctccgg cgtgaatgac ggggcggcgg cgctgatcat cgcctcggag gcagcggcga
     2701 agcgccatgg cttgacgccg cgcgcacggg ttgtggcgat ggtaaccgcc ggcgtcgccc
     2761 cgcgcatcat gggcatcggc cccgcgccgg cgaccgagaa gctgctggac cggctcggca
     2821 tgaaggtggg cgacatcgac gtgatcgagc tgaacgaggc gttcgcggcg cagggcttgg
     2881 cggtgacgcg ccagctcggc ctcccggatg atgctgagca cgtgaacccg catggcggcg
     2941 cgatcgccct cgggcatccg ctggggatgt ctggcgcgcg gctggcgctg accgcgctga
     3001 atgcgctgga gacgcgcggg cagaagcgcg cagttgccac catgtgcatc ggcgtcggcc
     3061 aggggatcgc ggcgctgatc gagcgcgttt aagaaaaatt caccgaccag cgcgcctatt
     3121 cgtcatggcc ggccttgtgc cggcgatccg tcgtggcacg gtaccgctac tgatggccgg
     3181 ggcgtggccc ggccatgacg gacacacagg agaccaacca tggccgcaat cgccgacatc
     3241 atcgcccgcg aagtgctcga tagccgcggc aatccgaccg tcgaagtgga tgtcgtgctg
     3301 gacagcggcg cgatggggcg ggcgatcgtg ccatccggtg cctcgaccgg cgcgcatgag
     3361 gcggtggagc tgcgcgatgg cgacaagtcg cgatttggcg gcaagggcgt gcagaccgcg
     3421 atcgccaatg tcgagggcga gatcttcgat gccatcggcg gcatggatcc gatcgagcag
     3481 gtaaagatcg acgagatcat gatcgatctc gacgggacgg cgaacaagtc gcggcttggg
     3541 gcgaacgcga ttctggcggt gtcgctggcg acggccaagg ctagtgcggc ggagctgggc
     3601 ctgccaatct atcgttatgt cggcggtgta tatgcgcgca cattgccggt gccgatgatg
     3661 aacatcgtca acggcggggt tcatgccgac aatccgatcg acatccagga attcatgatc
     3721 cagccaatcg gcgccggcac cgtggctgat ggcatccgca tgggttcgga aatctttgcc
     3781 tcgctgaaaa aggggctgca tgacgcaggt cacaacacca acgtcggtga cgagggcggt
     3841 tttgcgccca acctgaaatc cgctgacgaa gccctcggct tcattgccaa ggcctgcgag
     3901 accgcgggct atcgtcccgg cgaggatgtc acatttgcgc tggaccccgc ctccagcgag
     3961 ttcttcaagg atggcaagta tgccctggag ggcgaaggca agacgctcga tgccgacggc
     4021 atcgtcaagt actacgaagc gctgatcagc cgctatccga tcgtctcgat cgaggacggc
     4081 tgcgccgagg acgactggga aggttggaag catctgacag ccacgctggg caacaagttg
     4141 aacatcgtcg gcgacgatat cttcgtgacc aatcccgagc ggctgcgccg tggcataaag
     4201 gagcattcgg cgaatgccat cctgatcaag gtcaatcaga tcggcacgct gagcgaaaca
     4261 ctcgaaacga tggagattgc ccacaatgct ggcttccgct gcgtcatgag ccatcgctcc
     4321 ggcgaaaccg aggatgccac gatcgccgac ctggcggttg ccacgaactg tggccagatc
     4381 aagacgggca gcctctcccg cagcgatcgc accgcgaaat acaaccagtt gatgcgcatc
     4441 gaagcagagc tgggcccggc cgcccgctac gcaggccgca ccattcttcg caggtagaca
     4501 caggcgcatt tacgtcgcct tgcggggagg ttgccgccac ccccgggacc tgatccgcgc
     4561 gcggggcggg gagaggggcc ttccggagga acggattcca gtaccggcag tgcaatcagc
     4621 ggaaataaaa tcgtgtttcg cttgcgcacc ctcctggcag caatgtgacg agcaagctaa
     4681 ggtcttgttg cgtacaatca aatcaggagg gaagtatgtc acggatacgc gtagggctgg
     4741 ccgcttgtgg cctcgctctt gccgcgatgc tgtcgctggc cgacggcgcc cgcgcacaga
     4801 acgagttgaa aagccccacg atggacgcga tcaagcagcg cggtcagctc atctgcggca
     4861 tcgacaccgg catacctggt tacgcgtttc aggactccaa gggcgtgtgg cagggcctcg
     4921 atgtctccta ctgccgcgcc attgccgacg cgctgcttgg ctcgccggac aaggtgaagt
     4981 acgtcggcac cacgtcgaag gttcgcttct cggtgttggc ctctggtgag atcgacgtcc
     5041 tgatccgcga ctctgagcag acctttgttc ggaacacgca gctcggcctc gatgagcccg
     5101 cctacaattt cttcacggcg cagactttca tggtgcgcaa gagcctcgga gtggcgcacg
     5161 cgaaggacct gaacggcgca acgatctgcc tgctcaccgg cacaaccctg gagacgaaca
     5221 tcgccgactt caaccgggcg aacaacatca agatcaacac gctgctgttc gacaagccgg
     5281 aggaggcctt cgctgccgcc ga
//
LOCUS       DATGEM010000611         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1397281, whole genome shotgun sequence.
ACCESSION   DATGEM010000611 DATGEM010000000
VERSION     DATGEM010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397281"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1017
                     /locus_tag="VKQ27_18815"
     CDS             148..1017
                     /locus_tag="VKQ27_18815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY91043.1"
                     /translation="MDFLGFLVTQCLNALSQAALLFFLGVGLTLIFGIMRIVNFAHGA
                     FFMLGAYVGYSAVHLTGSFWASLVAAPLLVGGFGAAFELGILRRLYRRDHSAFLMVTF
                     GLTLVMGEAIRLSWGVAALEVPAPKSLSGIVFILDEPFPIYRLFLIGAGIVVALAIWQ
                     FLDRTRLGLLIRATSQNAEMVHALGTDVRLVRSAVFGIGCGLAGIGGVFAAPLVTASL
                     GMAPNAVIDTFVIVIIGGMGSFAGSLIGALLVAFVQVFGTYYFPDLALALMYLLMLIV
                     LVIRPGGLLGKEA"
     gene            1017..1946
                     /locus_tag="VKQ27_18820"
     CDS             1017..1946
                     /locus_tag="VKQ27_18820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY91044.1"
                     /translation="MRILAVVVCVLALAPAGLPPYAMTLLTEALILGLFAMSLDLMVG
                     YTRLLSFGHAAAYGLGAYAAGNLLLHTSIPLVFTILLAALCSGVVAIGVAWVCTLASG
                     VSFSMLTLAFAQLLYAVAFKWTAVTGASDGLAGIPRSPGPFGITFFEGRAGFYYLVLA
                     CLVAAFIFCRSLVRSPFGAVLRGIRENEAKTLSLGYNTRRYKIAVVAISFTLGGLAGA
                     LYAPFAGFTNTELLFWLFSGQVLIMVIVGGTGTLIGPIIGAAFFMLVSNLLSSWTDSW
                     ALFFGAIFILFVLFAPQGIWGLATRWRSLSSTG"
     gene            1919..2668
                     /locus_tag="VKQ27_18825"
     CDS             1919..2668
                     /locus_tag="VKQ27_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91045.1"
                     /translation="MALLELDGLTRRFGGIVAVDGVTMRVAAGEVRAVIGPNGAGKST
                     LFNLITGVTTPTSGTVRFDEHALTGLSAYSVCQRGVSRTFQITALFPEMTARENVRLS
                     AQARHARRWLPLGGRNVFADAETRADAALERLGLIHVAHRPAGLLTHGDQRLLEVAMA
                     LAQRPRLLLLDEPTQGLSVEETEQAVETLSALLADGSMTVLLVEHDMEVVFRLAHMIT
                     VLHRGAVIADGAPDEVRANEDVQRAYLGGLA"
     gene            2668..3363
                     /locus_tag="VKQ27_18830"
     CDS             2668..3363
                     /locus_tag="VKQ27_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91046.1"
                     /translation="MLAITGLNTHYGASHVLQGVELAVPRGRICSVLGRNGVGKTTTL
                     RTVMGLVAPSGGQVMLDGTDITGWPPHRVARAGVAYVPEGRLIFPDLTVVENIRVAER
                     KPSRAWPITRLMELFPSLRERAASRGGYLSGGEQQMLAIARALVSDPKVMLLDEPSQG
                     LAPLVVRELAEVIRRLPSEGVTILLIEQNLRLAEAVADEIHIMVKGRVVYAASPEQFR
                     AEEAEIRGRYLTV"
     gene            3379..4161
                     /locus_tag="VKQ27_18835"
     CDS             3379..4161
                     /locus_tag="VKQ27_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HLY91047.1"
                     /translation="MDLNLRGKTALITGASKGIGHASAEILAEEGVNVIMVSRTQADL
                     DAARDHIASRCNVRAEVHAFDLADSRRIDKLAELHPDIDILVNNAGAIPGGNLQEVNE
                     ARWREAWDLKVFGYINMCRRFYALMAQRRRGVIINILGMAGEKVDVGYIAGSTGNAGL
                     MAFTRTLGGAASADNLRVVGINPGAIATDRLITMMKKRAQDRLGDAERWNDLMKPLPF
                     ERAGTPEEIGWMVAFLASDKSAYTTGTIITIDGGAANRGPMF"
     gene            4161..4901
                     /locus_tag="VKQ27_18840"
     CDS             4161..4901
                     /locus_tag="VKQ27_18840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91048.1"
                     /translation="MNLQGKVAVITGASGGIGSATAHRFAAAGCRVVVGYNSRTEEAD
                     KLVVALTGTGHRSARMPMENTATLRDVAAMVEREYGRCDVLVNSAGFTRMIAHADLEA
                     LTDELIDAIFVANVRGPFATIRAFVPLMKKSDDAVIINISSGAAISGGGSNIAYGASK
                     AAIDTMTKSLARVLGPNIRVLSVSPGAVDTGFVPGRTTEMVEKAAQRAPLKRVVQADE
                     VAATIEAAVTLMTASTGSILLVEGGSLL"
     gene            complement(4957..>5226)
                     /locus_tag="VKQ27_18845"
     CDS             complement(4957..>5226)
                     /locus_tag="VKQ27_18845"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY91049.1"
                     /translation="TAELYGFADRGLLAPGYLADVNVIDLDGLALSAPRMVHDLPAGG
                     KRLMQKVHGYDATIVSGRVTWRGGEPTSDLPGRLVRGQDLSLRAA"
BASE COUNT          867 a         1637 c         1723 g          999 t
ORIGIN      
        1 gaaggtcggg aaggtaccgg aatacaagac gccgataccg cagatcatcg ccacgctgac
       61 cggtgacgac atcacaccac cctgccggca gatgacattc gaggactgat ggccgggtca
      121 agcccggcca tgacgggtaa atattgaatg gacttcctcg gcttcctcgt cacccagtgc
      181 cttaacgcac tttcgcaggc cgcactgctg ttcttcctcg gcgttggcct gacgctcatc
      241 ttcggcatca tgcgcatcgt caatttcgcg cacggcgcgt tcttcatgct gggcgcctat
      301 gtcggctact ccgcggtcca cctcaccggc agcttctggg catcgctggt cgccgccccg
      361 ctgctggtgg gtggcttcgg agccgccttc gaactgggca tcctgcggcg tctataccgg
      421 cgtgaccaca gtgccttcct gatggtgacg ttcggcctga cgttggtcat gggcgaggcc
      481 atccgcctgt cgtggggggt cgccgcgctg gaagtgccgg cgccgaagtc gctctccggc
      541 atcgtgttca ttctggacga gccgttcccg atctaccgcc tgttcctgat cggcgccggc
      601 atcgtcgtgg cactcgccat ctggcagttc ctggatcgca cgcggttggg ccttctgatc
      661 cgcgccacct cacagaacgc cgagatggtg cacgcgctcg gcaccgacgt gcgactggtg
      721 cgctcggcgg tgttcggcat cggctgcggc ctcgccggca tcggtggcgt gtttgcagca
      781 ccactggtca cggcatcact cggcatggcg ccgaacgcgg tgatcgacac gttcgttatt
      841 gtcatcatcg gcggcatggg cagcttcgcc ggctccctga tcggggcgct gctggttgct
      901 ttcgtgcagg tgttcggcac ttattacttc cccgacctcg cgctggccct gatgtatctg
      961 ctgatgctga tagtgctggt gatccgcccc ggtggattgc tgggcaagga ggcgtgatgc
     1021 gcatccttgc cgtcgtcgtc tgcgtgctgg cgctcgcccc cgcaggcctg ccgccatatg
     1081 ccatgacatt gttgaccgag gcgctgatcc tcggcctgtt cgccatgagc ctcgacctga
     1141 tggtgggcta tacgcgactg ctgtcattcg gtcacgcggc cgcctacggg cttggtgcct
     1201 acgcggctgg caacctgctg ctgcacacgt ccatcccgct cgtattcaca atattgctcg
     1261 ccgcactgtg cagtggcgtt gtggcgatcg gcgtcgcctg ggtctgcaca ctggcctccg
     1321 gtgtgtcctt ctccatgctc acgctggcct ttgcgcaatt gctctacgct gtggccttca
     1381 aatggaccgc tgtcaccggc gcttccgatg gcctcgccgg tataccgcgc agcccaggac
     1441 cgtttggtat caccttcttc gaaggacgcg ctggcttcta ctacctggtg ctcgcctgcc
     1501 tggtcgctgc cttcatcttc tgccgatcgc tggtgcgatc gccgttcggc gcggtgcttc
     1561 gtggcatccg cgagaacgag gcaaagacgc tgtcgctcgg ctacaacacg cgacgctaca
     1621 agatcgcggt ggtggcgatc tccttcacgc tgggcggact ggctggcgcg ctctacgcac
     1681 cgttcgccgg cttcaccaac accgagctgc tcttctggct gttctccggc caggtgctga
     1741 tcatggtgat cgtcggcggc accggcacgc tgatcggacc gatcatcggc gcggcgttct
     1801 tcatgcttgt gtccaacctg ctgagctcgt ggaccgattc ctgggcgctg ttcttcggcg
     1861 ccatcttcat cctgttcgtg ctgttcgcgc cgcagggcat ttggggactt gcgacgcgat
     1921 ggcgctcctt gagctcgacg ggctgacccg ccggtttggc ggcatcgtcg cggtcgatgg
     1981 cgtgacgatg cgcgtcgccg ccggcgaggt gcgcgcggtg atcggcccga acggtgccgg
     2041 caagtccacg ctgttcaacc tgatcaccgg cgtcaccaca ccaaccagcg gcacggtgcg
     2101 gttcgatgaa cacgcgctga cggggctgtc cgcctacagc gtgtgccagc gcggcgtatc
     2161 gcgcacgttc cagattaccg cgctattccc cgagatgacg gcccgcgaga acgtccggct
     2221 ttcggcacag gcgcgtcacg caaggcgctg gctgccattg ggggggcgca atgtgttcgc
     2281 cgacgcggaa acacgcgccg atgccgcgct ggagcggctt ggcctgatac atgtggcgca
     2341 tcgccccgcg ggcctgctga cgcatggtga tcagcgattg ctggaggtgg caatggcgct
     2401 ggcgcaacgg ccgcggttgc tgttgctgga tgaaccgact caaggcctgt cggtagaaga
     2461 gacggagcag gcggtggaaa cgctgagcgc cctgctggcg gatggctcga tgacagtgct
     2521 gctggtggag cacgacatgg aggtggtatt ccgcctcgcg cacatgatca ccgtgctgca
     2581 tcgcggcgcg gtaatcgctg acggcgcacc ggacgaagta cgagccaacg aagacgtgca
     2641 gcgcgcctat ctcggaggat tggcctgatg ctggccatca ccggcctcaa cacgcattac
     2701 ggcgcaagcc atgtgttgca gggtgtcgaa ctcgcagtgc cgcggggacg catctgttcg
     2761 gtgcttggcc ggaacggtgt tggcaagacc acgacattgc gcacagtgat ggggctggtg
     2821 gcgccgagtg gtgggcaggt gatgctggat ggcaccgaca tcactggatg gccgccgcac
     2881 cgcgtcgcac gtgccggcgt cgcatatgtg ccagaaggcc gcctgatctt tccggatctc
     2941 accgtggtcg agaatatccg cgtcgcggaa cgaaagccct cgcgtgcctg gcctataacg
     3001 cgactgatgg agctgtttcc gtcattgcgc gaacgggcgg cgagccgcgg cggctatcta
     3061 tcgggtggcg agcagcagat gctggcaatc gcgcgcgccc tggtgagcga tccgaaggtg
     3121 atgctgctgg atgaaccgag ccagggtctg gcgccgctcg tggtgcgcga gttggccgaa
     3181 gtgatccggc ggctgccgag cgaaggcgtg acgatcctgc tcatagagca gaacctgcgt
     3241 ttggccgagg cagtagcaga tgaaatccac atcatggtga aagggcgcgt ggtgtatgcg
     3301 gcctcacccg agcagtttcg tgccgaggaa gcggaaatcc gcggtcgtta cctgacagtt
     3361 tgaaagtgga gaggtgcaat ggacctgaac ctgcgcggca agacagcatt gatcaccggc
     3421 gcatcaaaag gcatcgggca tgcgtccgca gagatcctcg ccgaagaagg cgtgaacgtt
     3481 atcatggtgt cgcgcacgca ggccgatctg gatgcggcgc gcgaccacat cgcatctcgc
     3541 tgcaatgtgc gggccgaggt ccacgcgttc gacctcgcag acagtcgcag aattgacaag
     3601 ctggcggagt tgcatcccga catcgacatc ctggtgaaca acgccggtgc catccccggc
     3661 gggaacctgc aggaagtgaa cgaggcgcgc tggcgcgagg cgtgggacct gaaagtcttc
     3721 ggctacatca acatgtgccg ccgtttctat gcgttgatgg cccagcgccg gcgcggcgtg
     3781 atcatcaaca tactaggcat ggcaggcgag aaggtggatg tcggctacat cgccggttcc
     3841 accggcaacg ctgggctgat ggctttcacc aggacgctcg gcggcgccgc ctcggctgac
     3901 aatctgcgcg ttgtggggat caatcccggc gcgatcgcca ccgatcggct gatcaccatg
     3961 atgaagaagc gggcgcagga tcgcctcggc gatgcggagc ggtggaacga tctgatgaag
     4021 cccctgccct tcgagcgcgc cggcacaccc gaggagatcg gctggatggt cgccttcctg
     4081 gcatcggaca aatctgccta taccactggc acgatcatca ccatcgatgg aggggccgcg
     4141 aaccgcgggc caatgttctg atgaacctgc aaggaaaagt cgctgtcatc acaggcgcga
     4201 gcggcggcat cggctccgcc accgctcacc ggttcgccgc cgcgggatgc cgcgttgtcg
     4261 tcggctacaa cagccgcaca gaggaagccg acaagctcgt cgtggcactg accggcacgg
     4321 gccatcgctc cgcacgcatg ccaatggaga atacggcaac gctgcgcgac gtcgccgcga
     4381 tggtggagcg tgaatacggt cgctgtgacg tgctggtgaa ctcagcaggc ttcacgcgga
     4441 tgatcgcgca tgccgatctg gaagctctga cggacgaact gatcgatgcg atcttcgtag
     4501 ccaatgtgcg cgggcctttc gccaccatcc gggcgttcgt gccgttgatg aagaagtcgg
     4561 acgacgcggt gatcatcaac atatcatcgg gcgctgcgat ctccggtggc gggagcaaca
     4621 tcgcctacgg cgcttcgaag gcggcgatcg acacgatgac gaagtcgctg gcgcgtgtgc
     4681 tgggaccgaa catccgcgtg ctgtcggtct cgcccggcgc agtcgacaca ggcttcgtgc
     4741 cgggccgcac cacggagatg gtggagaagg cggcgcagcg cgcgcctctg aagcgcgttg
     4801 tccaggccga cgaggtggca gcaacgatag aggccgccgt cacactgatg acagcgagca
     4861 ccggcagcat cctgctggtt gaaggcggct cactgctcta actgtcattg cgcggaggcg
     4921 taagctgacg aagcaatctc gtccgggatt cgtcgctcac gcagctcgca atgacaggtc
     4981 ttgtccccgt accagccggc ctggcagatc actggtcggc tcgccgccgc gccacgtcac
     5041 gcgacccgat acgatcgtcg catcgtagcc gtgcaccttc tgcatcaggc gtttcccgcc
     5101 cgccggcagg tcatgcacca ttcggggcgc cgagagggcg agaccgtcga ggtcgatcac
     5161 gttgacgtcc gcgaggtagc cgggcgccag caggccgcga tcggcgaagc cgtacagctc
     5221 ggccgt
//
LOCUS       DATGEM010000612        13908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_269816, whole genome shotgun sequence.
ACCESSION   DATGEM010000612 DATGEM010000000
VERSION     DATGEM010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269816"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VKQ27_18850"
     CDS             <1..1004
                     /locus_tag="VKQ27_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLY91050.1"
                     /translation="LEHLSELAIRAHRFSPAKLQAVLAAMNKAVMLDMDIAISVYQDA
                     LLLEREQRGRKMDALLHSFEERTAALVGMVAAAATDLRSTAEALSGTTGSTTERATNV
                     AAAVEEASVNVQTVASAAEELHSSIAEIARQVAQSSAIAGKAVDDARRTDTIVKTLAS
                     GAQRIGDVVGLINSIAGQTNLLALNATIEAARAGDAGKGFAVVASEVKSLANQTAKAT
                     EDISKQISEIQTATGEAVQAIESIAGTIEELSTIASSIASAVEEQGNATQEIARNVHE
                     AASGTQHVSANIAGVSEGARDTSAAASRVLDAARKLSGQAEQLRGDVRTFISDVKAA"
     gene            complement(1001..1606)
                     /locus_tag="VKQ27_18855"
     CDS             complement(1001..1606)
                     /locus_tag="VKQ27_18855"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLY91051.1"
                     /translation="MSRNDTRIHRIAGRGITIHGDDIDTDQILPARYLKAITFAGMEA
                     HAFEDARAAAKAQGGEHPLDQMRFAGGSVLFVNRNFGCGSSREHAPQSLHRLGIAAVI
                     GESFGEIFAGNCVAIGMPCVQTDTETIAALQRINETAPQTEFVLDLVGQTISAGDRVF
                     PVSLAQGRRQQFLEGTWDPTAVLLAAGPLIEQTLAELHGRG"
     gene            complement(1603..2991)
                     /locus_tag="VKQ27_18860"
     CDS             complement(1603..2991)
                     /locus_tag="VKQ27_18860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLY91052.1"
                     /translation="MGQTLYEKVFDRHVVREVTPGQYQLLAGLHLLNEVSSPPAFEAL
                     RERGLKLRHPALTFGSSDHSISTSGQTDDFADPMAAQQVRAMGRNMAEFGANYFDPAA
                     GEHGIIHVVAPERGLTQPGTVIVCGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLLM
                     EKLKVRRIEITGTLAPGVYAKDVALHVIRVLGAKGGIGFAYEFGGDVVDRFSMEERMT
                     LCNLAIEAGARCGYVNPDQTTFDYLSGRDHAPRGTAWDRAVAEWQRVRSDEDAAYDDR
                     VVLRADEIEPMVTWGTSPDQSVAVGGNIPAAEGDVLEFMGLTEGQPIVGMRIDVAFIG
                     SCTNGRLSDFEAVAACLRDRDLHVAPHVRALVVPGSRRVREALVARGLDAVFRDAGFQ
                     FRDAGCSLCCGMNADRLEGREVCASSSNRNFRGRQGSPTGRTLLMSPVMVAAAAIAGE
                     VTDARQFFGAAQ"
     gene            3093..3752
                     /locus_tag="VKQ27_18865"
     CDS             3093..3752
                     /locus_tag="VKQ27_18865"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLY91053.1"
                     /translation="MLQIHGQLRDMILSLDIGPGERLTERWLESRFDGSRTPVRAALV
                     RLEAEGLVQRDGRNWIAAPIDLGEIEALAEFREPLEITAVRLACARATEADLDAIEEM
                     LNACRPGVPREEWHKVGTDFHVELARLSGNPFLVKAIEGVMTRLSRPRWLEVWTEASR
                     EQAWSEHRRILESIRAKQPVEASRETAEHIRDTRDRLLGSLNDDRRGLRARGFAIVGV
                     R"
     gene            complement(3749..4735)
                     /locus_tag="VKQ27_18870"
     CDS             complement(3749..4735)
                     /locus_tag="VKQ27_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY91054.1"
                     /translation="MQRQPFGRLGPVSTLTLGGGGLGMLWGPTSFDECVATVHAAVAA
                     GITLLDLAPRYGDGKAEEVVGAAFDGRLPDGVRVTSKCNLGNPPGDQVEGILRRSIED
                     SLRRLRASRLDLFFLHCNLAPMSNPMWGNPEALPRLTSYELFAEHVRPAFEKLVGEGL
                     IGAWGLTGIGHPDAIIRAIGEQPAPMAVQCIANLLDSPGGLKFFQGAAKPRTVMAAAH
                     ANVVGVMGIRAVQAGALTAAIDRPLPEEHPEVVDYQRAAAFRALCAELGENPAVLAHR
                     YAMSLDVDTLVLGVKNRAELAECVAAAAAGRLPARLMARIDESVRETEGRSI"
     gene            4865..5161
                     /gene="rplU"
                     /locus_tag="VKQ27_18875"
                     /pseudo
     CDS             4865..5161
                     /gene="rplU"
                     /locus_tag="VKQ27_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            5465..5734
                     /gene="rpmA"
                     /locus_tag="VKQ27_18880"
     CDS             5465..5734
                     /gene="rpmA"
                     /locus_tag="VKQ27_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLY91055.1"
                     /translation="MAHKKAGGSSRNGRDTAGRRLGIKKFGGESVIAGNIIVRQRGTK
                     WKPGHNVGLGKDHTIFALIDGKVTFKRRSDDRVHVSVAPLPAAAE"
     gene            5863..6879
                     /gene="obgE"
                     /locus_tag="VKQ27_18885"
     CDS             5863..6879
                     /gene="obgE"
                     /locus_tag="VKQ27_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLY91056.1"
                     /translation="MKFLDEAKIHLRSGDGGDGVVAFRREKYIEFGGPDGGNGGKGGD
                     IVFEAVDNLNTLIDFRYSQHFRAKKGGNGAGSDKTGAGAPDVVIKVPVGTQVLAEDRA
                     TLLADLDAPGTRVLLLRGGDGGFGNTHYKSSTNRAPRRADKGWPGEQRSVWLRLKLIA
                     DAGLVGLPNAGKSTFLAAASAARPKIADYPFTTLHPQLGVVRLSAAEEFVLADIPGLI
                     EGASEGAGLGTRFLGHVERCAVLLHLVDGAAGDVVRAWRTVRDELAAYGGGLADKPEL
                     IGLNKVDAMTAREASARRAALAKASGRPVMLLSGVTGQGVPEMLRALQNMITQAREER
                     AVAA"
     gene            6876..7991
                     /gene="proB"
                     /locus_tag="VKQ27_18890"
     CDS             6876..7991
                     /gene="proB"
                     /locus_tag="VKQ27_18890"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HLY91057.1"
                     /translation="MNPSLASAQRLVIKIGSALVVDQDEAAPRTAWLAGIAADIASLR
                     ARGVDVIVVSSGAIALARRSLGLHQARLRLEEKQAAAAVGQIRLAQAWSEALSAKGLT
                     AAQLLLTLEDTEDRRRYLNARATLNTLLGLNCIPVINENDTIATTEIRFGDNDRLAAR
                     VAEMTQSDQLILLSDIDGLYTADPRRDPGAAHIPTVSTITPEIEAMGGEPPPGYSSGG
                     MRTKLEAARIATQAGCAMAIAYGHTDRPLTALETGARCTWFLPSPEGRNARKRWIAGA
                     LAPLGTLVVDAGAARALAAGRSLLPAGVRAVEGSFERGDPVVVRGLDGKALARGLSAY
                     TNGDAERIAGHRSDEIEAILGWRGRDEIIHRDDLVLL"
     gene            8071..9330
                     /locus_tag="VKQ27_18895"
     CDS             8071..9330
                     /locus_tag="VKQ27_18895"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLY91058.1"
                     /translation="MHIETEPTQHLQRAAHAARHASHVLARTSDADRNGALRAMAASL
                     RARTAEILAANATDLATCTGTAAFRDRLALTEARVDAMAKGLEYIAALPDPLGRTLAD
                     WTRPNGLRIQRIATPIGVIGMIYESRPNVGADAAGICLKSGNAVILRGGSESVHSAAA
                     INAALVAGLRTAGLPEACVQIAPNADRAYVAAMLAAAGLLDLIVPRGGKSLVERVQRE
                     ARVPVLAHAEGLCHTYVHAAADFAMARAIIANAKMRRTGICGATETLLIDRAIAPALL
                     PAIVADLRAQGCDFRADAAARDIVPDLTPATEADFNTEWLDAILSVAVVDGVEAALAH
                     IARHGSEHTDAIVTEDPAAAQRFLNGVDSAVALWNASTQFCDGGEFGFGAEIGIATGR
                     IHARGPVGPEQLTTYRYLVLGTGQVRP"
     gene            9393..9947
                     /locus_tag="VKQ27_18900"
     CDS             9393..9947
                     /locus_tag="VKQ27_18900"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HLY91059.1"
                     /translation="MLGGSFNPAHPGHRHVIDVARRRLHLDQVWLLVSPGNPLKPHRG
                     MAPFQQRLASARCIADGRRVIASAIEATFHTHYSIDTLRLLRHRFPRARFIWLMGADI
                     LEQLPLWRRWLDFARRVPIAVLPRPSYNYRALASLAARRLTRHRRPERAAPVLSELAA
                     PAWIFLCVPQHTASATAIRAGAGS"
     gene            10019..10450
                     /gene="rsfS"
                     /locus_tag="VKQ27_18905"
     CDS             10019..10450
                     /gene="rsfS"
                     /locus_tag="VKQ27_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HLY91060.1"
                     /translation="MPEAGKTPGTPRKKTIAAGPKKSAVRVKAEPSALDKLQSVIVGS
                     LEDDKAENVVTLDLAGKASFCDRMVIATGLADRQIAAMAEHLSEKLHQAGLKRVQVEG
                     AGGADWMLIDAGDIIVHLFKPEARAMYGLERMWGAELDEVG"
     gene            complement(10580..11467)
                     /locus_tag="VKQ27_18910"
     CDS             complement(10580..11467)
                     /locus_tag="VKQ27_18910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY91061.1"
                     /translation="MRILFVTSNRLGDAVLSTGLLDHLVREHPDASITVACGPVAEGV
                     FARMPNRVRTIVLAKRPYSLHWLPLWVSTATTVWDLVVDIRGSLLAWMVLSRRRAVMR
                     PRSGHKVAQLAAVLGLDPPPLPVAWTAPEDRARAAALLPAGRQIIALGPTANWVPKVW
                     PADRFAALFHALDRGAVPAVFAGPGETERAMAAPLLAALPGAIDLVGRLTLPEVAACL
                     ARCTLFVGNDSGLMHLAAAAGTPTLGLFGPTSAAEYAPAGPRAAVALSSSARMEDLSV
                     ETAVQAASRLLERATLVQA"
     gene            11908..>13908
                     /locus_tag="VKQ27_18915"
     CDS             11908..>13908
                     /locus_tag="VKQ27_18915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY91062.1"
                     /translation="MELLERDLQLKALATALDGAAAGEGRIAVVSGEAGIGKTSFVER
                     FLTDRRAAVRVLKGNCDSLFHPSPLGPLYDIAYQTGGRLLAQLEVETPRAALFATILG
                     ELDGSHQPTILLFEDVHWADEATLDLIKFLSRRIAHIRVLIILTHRDDGAAPLLRLLL
                     GDLATSRSTIRIALPRLTIDAVRTLIAERPFDPATVHGQTAGNPFFVTEVLANTASGI
                     PATVRDAVLARTGTLQPNARHVLEATAVLGSRMDHGVLAGMLDGDMDALADCMTVGLL
                     EATADGVAFRHELVRDTILHQLDPVRRRVLCRLAFDALKASGIGRGDFAQLAELAAGA
                     GDSEAVLEYGLAAARADAAVGAHRAAAAQYLRVLECAGDRPLNERAQLFETYAEECAI
                     VDNQREAIRGRREAIELWRLAGDSVREGANLSALAWPLVRSGQNLAAHETSKRGLALL
                     ESLPPSHELADAYRMHAHLRMLDRDRPLAVELGSKAIALAKILGDKRTIVAAEIVVGS
                     ALLVDGDEAGADHLNRALAMAREEGWDALAGLAYLNIGSSYGEQYQFAEAEHYLVEGI
                     DYARERDLDHATHYMTAWLALVRLYQGRWSEALELAEWVVGRPGTSAITRIMALIALG
                     RLRIRRGEPGTEQVLDEALVLALQTDTLQRLAPVRSARAEAAW"
BASE COUNT         2402 a         4981 c         4381 g         2144 t
ORIGIN      
        1 tcctggagca cttgagcgaa ctggccatcc gcgcacaccg cttctcaccg gcgaagctgc
       61 aggcagtgct ggccgcgatg aacaaggccg tcatgctcga catggacatt gcgatttcag
      121 tctatcagga cgccctgttg ctcgagcgcg agcaacgcgg ccgcaaaatg gacgctctgc
      181 tgcactcctt cgaggaaagg actgccgcct tggtcggcat ggtcgccgcg gcagccaccg
      241 accttcgctc caccgcagag gcgctctctg gcaccaccgg ctcgaccacg gaacgggcga
      301 ccaatgtcgc ggcggcggtc gaagaggcaa gtgtcaacgt gcagaccgtg gcgtccgccg
      361 ccgaggagct ccacagctcg atcgccgaaa tcgcgcgtca ggtcgcgcaa tcttccgcca
      421 tcgccggcaa ggcggtggac gacgctcgcc gcaccgacac catcgtcaag actctggcga
      481 gcggcgcgca gcggatcggc gacgtggtcg gcctgatcaa cagcattgcc ggccagacca
      541 acctcttggc actcaatgcg acgatcgagg cggccagggc cggcgacgcc ggcaaaggct
      601 ttgccgtcgt cgcttccgag gtgaagagcc tggccaatca gaccgccaag gcaaccgagg
      661 acatcagcaa gcagatctcg gaaatccaga ccgcgaccgg tgaagcagtg caggcaatcg
      721 agtcgatcgc cggaacgatc gaggagctca gcacgatcgc ctcatcgatc gcctcggcgg
      781 tggaagagca aggcaacgcg acgcaggaga tcgcgcgcaa cgtgcacgag gccgcaagcg
      841 gaacacagca cgtcagcgcg aacatcgccg gcgtcagcga aggcgccagg gacaccagcg
      901 ccgcagccag ccgcgtactc gatgccgccc gcaagctgtc gggtcaggcg gaacaattgc
      961 gcggcgatgt ccgcacgttc atctccgacg tgaaggccgc ttagccgcga ccatgcaact
     1021 cggcgagcgt ctgttcgatc aatggcccgg cggccagcag caccgcggtc gggtcccacg
     1081 tcccttccag gaactgctgg cgtcgccctt gcgccagtga gaccggaaaa acgcggtcac
     1141 ccgcgctgat cgtctgcccg accagatcga gcacgaactc ggtctgcggc gccgtctcgt
     1201 tgatccgctg caacgccgcg atcgtctcgg tgtcggtctg gacgcagggc atgccgatgg
     1261 cgacgcagtt gccggcaaag atctcgccga acgactcacc gatcaccgcg gcaattccca
     1321 gccgatgcag gctctgcggc gcgtgctcgc gcgatgaccc gcagccgaaa ttgcggttga
     1381 cgaacagtac cgacccgccg gcgaaacgca tctgatcgag cggatgctcc ccgccctgcg
     1441 ccttcgccgc ggcgcgcgcg tcctcgaatg cgtgcgcctc catcccggca aaggtgatcg
     1501 ccttcagata gcgcgcgggc aggatctggt cggtgtcgat atcgtcgcca tggatggtga
     1561 tgccgcggcc ggcgatgcgg tggatacgtg tgtcattacg gctcattgcg ctgctccgaa
     1621 gaattgcctg gcgtcggtga cttcacccgc gattgccgcg gcggcgacca tcaccggcga
     1681 catcagcagg gtgcggccgg tgggcgatcc ctgccgcccg cgaaagttcc ggttggacga
     1741 ggaggcgcag acctcccgcc cctccagcct gtcggcgttc atgccgcagc acagcgagca
     1801 tccggcatcg cggaactgga acccggcgtc gcggaacacc gcatccagtc cgcgtgccac
     1861 cagagcttcc cgcacgcggc gcgatcccgg cacgaccagg gcacgcacat gcggcgcgac
     1921 atgcaggtcg cgatcgcgca ggcacgccgc aaccgcctcg aagtccgaca gccgcccgtt
     1981 ggtgcaggag ccgatgaacg cgacatcgat gcgcatgccg acaatcggct gtccctcggt
     2041 caggcccatg aattccagca cgtcaccttc ggcagccggg atgttgccgc ccaccgccac
     2101 cgactgatcg ggcgaggtgc cccacgtcac catcggctcg atctcgtccg ctcgcagcac
     2161 cacccgatcg tcgtacgcag catcctcgtc cgagcggaca cgctgccatt ccgccaccgc
     2221 gcgatcccat gccgtgccgc gcggcgcatg atcgcgcccg ctcaggtaat cgaacgtcgt
     2281 ctggtccgga ttgacatagc cgcaccgcgc ccccgcctcg atggcgaggt tgcacagcgt
     2341 catcctctcc tccatcgaaa accggtcgac gacatcgccg ccgaactcgt aagcgaaccc
     2401 gatgccgccc ttggcgccaa gcacgcggat cacgtgcagc gccacgtcct ttgcatacac
     2461 accgggcgcc agcgtgccgg tgatctcgat ccgccgcacc ttcagctttt ccatcagcag
     2521 cgtctggctg gcgagcacat cgcgcacctg cgacgtgccg atgccgaacg caatcgcgcc
     2581 gaacgccccg tgcgtggatg tgtggctgtc gccgcagaca atcaccgttc ccggctgagt
     2641 gagcccacgc tcgggggcaa ccacatggat gatgccgtgc tcccccgcgg cagggtcgaa
     2701 atagttggcg ccgaactccg ccatgttgcg ccccatcgcc cgcacctgct gggcggccat
     2761 cggatcggcg aagtcgtcgg tctgcccgct ggtcgagatc gagtggtccg aactgccgaa
     2821 ggtcaacgcc ggatgccgca gcttcagtcc gcgctcgcgg agcgcctcga acgccggcgg
     2881 gctggagacc tcgttgagaa gatggagccc ggccagcaac tggtactgcc cgggtgttac
     2941 ctcccgaacc acgtgacggt cgaacacctt ctcgtacagc gtctggccca tctgtctcct
     3001 ccaatttggc atcccatttg ggataccaaa ttgggataca gtttgccata cccaaatggg
     3061 atgcaatttt gtatcctgcc ccgatgtcgc aaatgctgca gatccacggc caactccggg
     3121 acatgatcct gtcgctggat atcggccccg gagagcgcct caccgagcgc tggctggaat
     3181 cacgcttcga cggctcgcga acgcccgttc gtgcggctct cgtgcgcctg gaagccgagg
     3241 gccttgtcca gcgcgacggc cgcaactgga tcgccgcacc catcgacctg ggcgaaatcg
     3301 aggccctggc cgaattccgt gaacccctcg agatcaccgc tgtccgcctg gcctgcgccc
     3361 gcgccaccga ggccgacttg gacgcaatcg aggagatgct gaacgcctgc cgccccggcg
     3421 ttccccgcga ggagtggcac aaggtcggca ccgacttcca cgtcgagctc gcccgcctgt
     3481 ccggcaaccc attcctggtc aaggcgatcg agggcgtcat gacacgcctc tctcgccccc
     3541 gctggctgga agtctggacc gaagcctccc gcgaacaggc ctggtccgag caccgccgca
     3601 tcctggaaag catccgcgcc aaacagccgg tcgaggcatc gcgcgagacc gccgaacaca
     3661 tccgcgacac gcgtgatcgt ctgctcggct ccctgaacga tgaccgccgc ggcctccgcg
     3721 cccgtgggtt cgcgatcgtg ggcgtccgct aaattgagcg gccctcggtc tcccgcaccg
     3781 actcgtcgat ccgcgccatc agccttgctg gcaatcgtcc cgccgccgct gccgccacgc
     3841 actccgccag ttccgcccgg ttcttcaccc ccagaaccag ggtgtccaca tccagcgaca
     3901 tcgcataccg atgcgcgagc accgccgggt tctcccccag ttccgcacac agcgcccgaa
     3961 acgcagccgc acgctggtag tccactactt ccggatgctc ctccggtagt ggcctgtcga
     4021 tcgccgccgt cagggcgccc gcctgcaccg cgcggatgcc catgactcca accacgttgg
     4081 catgcgccgc cgccatcacg gtccgcggtt tcgccgctcc ctggaagaac ttcaggccgc
     4141 ccggcgaatc cagcaagttg gcaatacact gcaccgccat tggcgccggc tgttccccaa
     4201 tcgcgcggat gatggcatcg ggatgcccga tccccgtcag accccaggcg cctatcagcc
     4261 cttcccccac cagcttctcg aacgccggac gcacatgttc tgcgaacagc tcgtaagacg
     4321 tcagcctagg caatgcctct ggatttcccc acatcggatt ggacatcggc gccagattgc
     4381 aatggaggaa gaacagatcc agccgggacg cccgcagccg ccgcaggctg tcctcgatcg
     4441 aacggcgaag tatcccctcc acctgatccc caggtgggtt accgagattg cacttgctgg
     4501 tcacgcgcac cccgtccggc agcctgccat cgaacgctgc gcccaccacc tcctcggcct
     4561 tgccatcgcc atagcgaggc gccaggtcga gcagcgtgat tccggccgca accgccgcgt
     4621 gcaccgtggc gacgcattcg tcgaaactgg ttggccccca cagcattcct agcccgccgc
     4681 cgcccaatgt gagtgtagag accggcccaa gccgcccgaa tggttgccgc tgcatgcaat
     4741 ttcctcttct gttggttgta tcatgcacct tgcgcgttga cagcgcgatt tcggtcgcta
     4801 taacgcccgc tctccggccg ctggggggtt cctggcggcc cgcgtggttt tgggacaccg
     4861 tgtgatgttt gcagtcatcc gcaccggcgg aaaacagtac cgggttacgc caaatgcggt
     4921 gctgaaggtc gagaagctgg acgcggagcc tggcacgacc ttcaccttca ccgacgtgct
     4981 agcgcttggc aatgatgaca gcgtgaccat tggcgcgcct gtcgtggcgg gcgcggcggt
     5041 cactgcgacc gtgatcgccc aggaccgtct ggacaaggtc atcatcttca agaagcgccg
     5101 ccggcagaac agccgccgca agaacggcca tcgccagcac gtgaccgtgc tgcgcgtatc
     5161 gggcttcgag aaggacggca tgcccctggt cgccaccgcc gcgtcctccg aagcgtcagg
     5221 agcgcagctt gaagcggcgg aaggcgaggg tgtcgtctac ggcgagacgc cggcaggccg
     5281 tcccgagcac catcctgcgg caccatccgc cgccgcgacg ccgccggcag aacaccaccc
     5341 ccatcatccg ccgatgcacg tcaccgagcc tgacaatccg gccggcctcg aggcagcgga
     5401 aggcgaggcc atcgtcgaag gcgacacacc cgagcccgac gccgagcgga aggagtaagc
     5461 accgatggca cacaaaaagg ctggcggttc ctcccgcaac ggccgcgata ccgcaggccg
     5521 gcggctcggc atcaagaaat ttggcggcga gagcgtgatc gccggcaaca tcatcgtccg
     5581 ccagcgtggc accaagtgga agccgggcca caatgtcggc ctgggcaagg accacacgat
     5641 cttcgcgctg atcgacggca aggtgacgtt caagcgccgg tcggatgacc gtgtgcacgt
     5701 ctccgtcgca ccgctgccgg ctgccgccga gtaaggcgcc ggacccgttt gtgtttcacg
     5761 cggggtcggc gcaagccggc cccgtttctg tatggtcgcg ggcgacgccc gctcttaact
     5821 cgtcatggcc gggcttgacc cggccatccc ctggagcacc cgatgaagtt tctcgatgag
     5881 gccaagatcc acctccgctc cggcgacggc ggcgacggtg tggtcgcgtt ccgccgcgag
     5941 aaatacattg agttcggcgg ccccgatggc ggcaacggcg gcaagggcgg cgacatcgtg
     6001 ttcgaggctg tggacaacct gaacaccctg atcgacttcc gctactcaca gcatttccgg
     6061 gcgaagaagg gcggcaacgg cgctggatcc gacaagaccg gggctggcgc gccggacgtg
     6121 gtgatcaagg tccccgtcgg cacgcaggtg ctggcggagg accgcgcaac actgctcgcc
     6181 gacctggatg cgcccggcac ccgggtgttg ctgttgcgcg gcggcgacgg cgggttcggc
     6241 aatacgcact ataagtcgtc caccaaccgc gctccccggc gcgccgacaa gggctggccg
     6301 ggtgagcagc gctcggtctg gctgcgcctc aagctgatcg ccgatgccgg actggtcggg
     6361 ctgcccaatg ctggcaaatc caccttcctc gcagcagcgt ccgcggcgag gccgaagatc
     6421 gcggactatc ccttcaccac acttcacccc cagttgggcg tggtgcgtct gtccgccgcc
     6481 gaggagttcg tgctggccga cattcccggc ctgatcgagg gcgcctcgga aggagccggt
     6541 ctaggcaccc gcttcctcgg ccatgtcgag cgctgcgccg tgctgctgca cctggtcgac
     6601 ggtgcggccg gcgacgtggt gcgtgcctgg cgaacggtgc gcgacgagct ggcggcatat
     6661 ggcggtggct tggcggacaa gccggagttg attggactga acaaggttga tgcgatgaca
     6721 gcgcgcgagg cttctgcgcg gcgggccgcg ctggccaagg cgtcgggccg accggtcatg
     6781 ctgttatccg gcgtgaccgg acagggcgtg ccggagatgc tgcgcgcact ccagaatatg
     6841 atcacgcagg cacgcgagga acgggcagtg gccgcatgaa cccttccctc gcctccgccc
     6901 agcgcttggt catcaagatc ggcagcgccc tggtcgtgga ccaggacgag gccgcccccc
     6961 gcaccgcctg gctcgccggc atcgccgccg acatcgcctc cctgcgcgct cgcggcgtgg
     7021 acgtgattgt cgtgtcgtcc ggcgccatcg cactcgcccg ccgctcgctg ggcctgcatc
     7081 aggcacgcct gcgcctcgag gagaagcagg ctgccgccgc cgtcggccag atccgtctcg
     7141 cccaggcctg gagcgaggca ctctccgcca agggcctcac cgccgcccag cttctcctca
     7201 ccttggaaga caccgaggac cgccggcgct acctgaatgc ccgcgctacc ctgaacacgc
     7261 tgctcggcct gaactgcatc ccggtcatca acgagaacga cacgatcgcc accaccgaga
     7321 tccgcttcgg cgataacgac cggctcgccg cgcgcgtcgc tgaaatgacc cagtctgacc
     7381 aactgatcct gctgtccgac atcgatggcc tctacaccgc ggacccgcgc cgcgacccag
     7441 gcgccgcgca cattccgaca gtctccacca tcactccgga gatcgaggca atgggcggcg
     7501 aaccgccccc cggctactcg tccggcggca tgcggaccaa gcttgaggcc gcgcgcattg
     7561 cgacacaggc cggctgcgcc atggccatcg cctacggcca taccgaccgc ccgctgaccg
     7621 ccctggagac aggcgcgcgc tgcacctggt tcctcccgtc gcccgagggc cgcaacgccc
     7681 gcaagcgatg gatcgcaggt gcactggcgc cgttgggaac gctggtggtc gatgccggtg
     7741 ccgcgcgcgc cctcgccgcc ggccgttcgc tcctgccagc cggcgtgcgc gccgtggaag
     7801 gctcgttcga acgcggcgat cccgtggtgg tgcgtggcct ggatggcaag gccctggcgc
     7861 gtggtctctc cgcctacacc aacggcgacg ccgaacgcat cgccggccac cgctcggacg
     7921 agatcgaagc gatccttggc tggcgaggcc gggacgagat catccaccgc gacgatctgg
     7981 tgctgctgta gtgccgcctt tgatcggcta acgctgccta ctggaaacga ggccgctgcg
     8041 acgtcactat aggggttgag gatccagtcg atgcacatcg agaccgaacc cacccagcac
     8101 ctgcaacgcg ccgcccatgc cgcgcgccat gcctcgcatg tgctggcgcg cacctcggac
     8161 gcggaccgca acggcgccct ccgcgccatg gccgcatcgc tgcgcgcccg caccgcggag
     8221 atcctcgccg ccaacgccac ggacctggct acctgcaccg gcaccgctgc cttccgcgac
     8281 cgcctcgccc tcaccgaagc gcgtgtcgac gccatggcca aaggcctgga atacatcgcg
     8341 gccctgccgg atccgctcgg ccgcacgctc gccgactgga ctcgccccaa cggcctgcgc
     8401 attcagcgca tcgccacgcc catcggcgtc atcggcatga tctacgagag ccgccccaac
     8461 gtcggcgccg acgccgccgg catttgcctg aagtcgggca acgcggtcat cctgcgcggc
     8521 ggctcggaaa gcgtgcactc cgccgccgcc atcaacgcag cgctggtcgc cggcctgcgc
     8581 accgccggcc tccctgaagc ctgcgtgcag atcgccccca acgccgaccg cgcttatgtc
     8641 gcggcgatgc tcgccgccgc cggcctgctg gatctgatcg tgccccgcgg cggcaagtcg
     8701 ctggtcgaac gcgtccaacg cgaggcccgc gtccccgtcc tggcccacgc cgagggcctc
     8761 tgccacacct acgtccacgc ggcagccgac ttcgccatgg cccgcgccat catcgccaac
     8821 gcaaagatgc gccgcaccgg catctgcggc gcaactgaga ccctgctgat cgaccgcgcc
     8881 atcgcccccg ccctcctccc agccatcgtc gccgacctgc gcgcccaagg ctgcgacttc
     8941 cgcgccgacg ccgccgcgcg cgacatcgtc cccgacctca ccccggccac cgaggcggac
     9001 ttcaacaccg aatggctcga tgccatcctg tccgtcgccg tggtcgatgg ggtggaggcc
     9061 gcgctcgctc acatcgcccg ccacggctcc gagcacaccg atgccatcgt cacggaagac
     9121 cccgccgccg cgcagcgctt cctcaacggc gtggacagtg cggtggccct gtggaacgcc
     9181 tccacccagt tctgcgacgg cggcgagttc ggcttcggtg ccgagatcgg catcgccacc
     9241 ggccgcatcc acgcgcgcgg cccagttggt cccgaacagc tcaccaccta ccgctacctg
     9301 gttctcggca ccggtcaggt gcgcccctga acaagcccgt ctggcacgaa aggctgccgc
     9361 gcttcggcga tcgccggcgc acccgtatcg gcttgctcgg cggctccttc aatccggcgc
     9421 accccggcca ccggcacgtc atcgacgtcg cccgcagacg cctccatctc gatcaggtct
     9481 ggctccttgt ctcgcccggc aatcccctga agccgcaccg cggcatggcg cccttccagc
     9541 agcgtttggc ctctgctcgc tgtatcgccg acggccggcg cgtcattgcc agcgcgatag
     9601 aggccacctt ccatacccat tactcgatcg acacgctgcg cctgctccgc caccgtttcc
     9661 cgcgggcgcg cttcatctgg ctgatgggcg ccgacatctt ggagcagctg ccgctctggc
     9721 gccgctggct ggatttcgcc cgccgtgtcc cgatcgcggt ccttccccgc ccgagctaca
     9781 actatcgcgc cctggcgagc cttgccgccc gcaggctaac gcgccaccgc cgcccggaac
     9841 gcgccgcgcc cgtgctcagc gagcttgcgg cgcccgcctg gattttccta tgcgtgccgc
     9901 agcacaccgc ctcggccacc gcgattcgcg caggagcagg gtcatagcca agccgctgaa
     9961 gccattctcg cccggccgac atcgcgcggc gccgcgcaag aaggtcgcca agcccacatt
    10021 gccggaggcc gggaagacgc ctggcacgcc gcgcaagaag acgatcgcgg cggggccgaa
    10081 gaaatcggcc gtccgggtga aggcggagcc gtcggcgctg gacaagctgc aatccgtcat
    10141 cgtcggcagc ctggaggacg ataaggccga gaacgtggtg acgttggatc tggccggcaa
    10201 agcctcgttc tgcgaccgca tggtgatcgc gacgggcctc gccgaccgcc agatcgccgc
    10261 gatggcggag cacctgagcg aaaaactcca ccaggccggc ctgaagcgcg tgcaggttga
    10321 aggcgccggc ggcgcggact ggatgctgat cgacgccggc gacatcatcg tgcacctgtt
    10381 caagccggag gcgcgcgcga tgtatggtct ggagcgcatg tggggtgccg aattggacga
    10441 ggtcggctag taccctccgt cactggagca cgagtcgcgc gttcccgtca tgaccggccg
    10501 ccgtgcccgc catcagtggc tgcagaatat cgcgacggat agccccaaca agcacggaca
    10561 cgaccggatg ttccgggtac taggcctgca ccagcgttgc gcgttccagc aatcgtgacg
    10621 ctgcctggac agcggtttcc acggacaaat cctccattcg cgccgagcta gacagggcca
    10681 cagcggcgcg cggaccagcc ggggcatatt ccgccgctga cgtaggcccg aacagcccca
    10741 gcgtcggcgt cccggcggcg gcagcgagat gcatcaggcc ggagtcgttg ccgacgaaca
    10801 gcgtacaccg cgccaggcag gccgcaactt ccggcagtgt caggcgaccg acgagatcga
    10861 ttgcgccagg aagcgccgca agcagtggcg ctgccatcgc acgctccgtc tcgccggggc
    10921 cagcgaacac agcaggcacc gcaccgcggt cgagcgcatg gaacagggct gcgaagcgat
    10981 ctgctggcca gaccttcgga acccagtttg ccgtggggcc gagcgcgatg atctggcgac
    11041 cggccggaag cagggccgcg gcgcgggcac ggtcctcggg tgccgtccag gccaccggca
    11101 gaggtggggg atccagaccc agtaccgccg caagctgggc caccttatgc ccggaacgcg
    11161 gacgcatcac cgcgcgacgg cggctcagca ccatccaggc aagcaacgag ccgcgtatgt
    11221 ccacgaccag atcccagacc gtcgtcgcgg tggaaaccca gagcggcagc cagtgcaaac
    11281 tgtacggacg ctttgccagc acgatggtgc gaacgcggtt gggcatgcgc gcaaacacgc
    11341 cctcggcgac cggtccgcag gcgacggtga tgctggcatc gggatgttcg cgaaccagat
    11401 ggtcgagcag tccggtggac agcaccgcat caccgaggcg attcgaggta acgaacagaa
    11461 tgcgcattcc gtcgaactag acctgcccgg cgcgtggcaa acagagccgg caagacccac
    11521 cgcctgtgcc gtcgtcgcag gtcttttctg tgtccatcta tagcctgccc cggaccccga
    11581 tccggggctc atctgtggtt ccaaatttcg ctttagcagt caccgcggcg ccgcgcgaga
    11641 attgcgatcc gccaagtatg cccatcgctg cacgcgcccc tgcgctgcgc tggttgggta
    11701 tgttcaacac gccgatgcaa tgtgaaatat cggcaacgat gcaggcggtg gcagcgataa
    11761 tgccacccgg gggatgacca gcatgccggc catcacgaca aatcagtcaa acctgtgaac
    11821 gacgtcacag ccacactcgt gttcgcggtc caccagcggc gcatgcaagt caaccgacgc
    11881 agggcgcggg ggtgagagtc gcccgccatg gaactacttg aacgcgacct ccagttgaag
    11941 gcactggcga cggccctcga tggagccgcc gccggggaag gacgcattgc cgtcgtcagc
    12001 ggtgaagcgg gtatcggcaa aacctcattc gtcgaacgct tcctcaccga tcgccgcgcg
    12061 gcggtccgcg tgctgaaggg caactgcgac tcgcttttcc acccctcgcc gctcgggccg
    12121 ctctacgaca tcgcctacca gaccggcggc cgtcttctag cgcagctcga agtcgaaacg
    12181 ccgcgcgccg ccctgttcgc gaccatcctc ggtgagctcg acggttccca ccagccaacc
    12241 atcctgctgt tcgaggatgt acactgggca gacgaagcaa cactcgatct gatcaagttc
    12301 ctcagccggc gcatcgcgca tatcagggtg ctgatcatcc tgacgcaccg cgacgatggc
    12361 gcggccccgc tgcttcggct cctgctgggc gatctggcaa cgtcgcgttc aaccatccgc
    12421 atcgcgctgc cgcgcctcac gatcgatgca gtacgcaccc tcattgccga acggccgttc
    12481 gatcccgcga cggtgcatgg gcagacggca ggcaacccgt tcttcgtcac ggaagtcctg
    12541 gccaataccg cttccggcat cccagccacg gtgcgcgacg cggtgctggc gcgcaccggg
    12601 acattgcagc cgaatgcgcg acatgtgctg gaggcaaccg ccgtgctggg cagccggatg
    12661 gaccacggcg tgctggcagg aatgctcgac ggcgacatgg acgccctggc tgattgcatg
    12721 acagtcggcc tgcttgaggc aaccgccgac ggcgtcgcat tccgccatga actggtgcgc
    12781 gacactatcc tgcaccaact cgatccggtg cgccggcgcg tgctgtgccg gctggcattc
    12841 gatgcactga aggcctccgg catcggccgt ggcgacttcg cacagctagc ggaactcgct
    12901 gccggcgccg gcgatagcga ggctgtgctg gaatacgggc tcgccgcggc acgcgccgat
    12961 gccgccgttg gcgcccatcg tgctgccgcc gcgcagtatc tgcgagtgct ggaatgcgcc
    13021 ggcgatcggc cattgaacga acgagcgcag ctcttcgaga cctatgccga agagtgcgcc
    13081 atcgtcgaca accagcggga agcaatacgc ggacgccgcg aggcgatcga actgtggcgg
    13141 ctggctggag atagcgtgcg ggaaggtgcg aacctctccg cgctggcgtg gccactggtg
    13201 cgcagcggcc agaacctggc cgcgcatgag accagcaagc gtgggctcgc acttctggaa
    13261 tcgctgccgc cgtcgcacga actcgccgac gcttatcgca tgcatgcgca tctccgcatg
    13321 ctggatcgcg accggccgct ggcagtggag cttggctcca aggccatcgc gctggcgaag
    13381 atccttggcg acaaacgcac gattgtcgcc gccgagattg tcgttggctc cgctctgctg
    13441 gtggatggcg acgaagccgg tgcggaccat ttgaaccgcg ctctcgcaat ggcacgcgag
    13501 gaaggttggg acgcgcttgc cggtcttgcc tatctgaaca tcggctcaag ctatggcgag
    13561 cagtaccagt tcgcggaagc cgagcattat ctggtcgaag gcatcgacta cgcgcgcgaa
    13621 cgcgatctgg atcacgcgac gcactacatg actgcgtggc tcgccctggt gcgtctgtac
    13681 caaggacgat ggagcgaggc tctggaactg gccgagtggg tcgtcggacg gcctggcacg
    13741 tcggcgatca cacggatcat ggcgcttatc gccttggggc gcctgcgcat acggcgcggt
    13801 gagccgggta ccgagcaggt gctcgacgaa gcgttggtac tcgcactgca gaccgatacg
    13861 ttgcaacgct tggcacccgt ccgttcggca cgcgccgaag cagcctgg
//
LOCUS       DATGEM010000613         8240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1181424, whole genome shotgun sequence.
ACCESSION   DATGEM010000613 DATGEM010000000
VERSION     DATGEM010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181424"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..802
                     /locus_tag="VKQ27_18920"
     CDS             20..802
                     /locus_tag="VKQ27_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HLY91063.1"
                     /translation="MSCGSKDENADADKPVAPVEDLYNNGVDALNSQRYDSANDQFNL
                     VEQNYPYSTWAVNAQLMQGYTQYLQNKYTDAIGTLDRFIQLHPTHRDVAYAYYLRALC
                     YYEQIADIQRDQRGTQLAMNALQEVVNRFPDSAYARDARLKIDLCNDHLAGKEMDIGR
                     WYESQHLYTAAIGRFQRVVDDYQTTNHVPEALHRLTEIYLILGLKDQARKTASVLGYN
                     YPGSSWYEDSYNDLFDAGLVNQQGPTPAPQRGFISRAWHSLF"
     gene            830..2521
                     /gene="recN"
                     /locus_tag="VKQ27_18925"
     CDS             830..2521
                     /gene="recN"
                     /locus_tag="VKQ27_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HLY91064.1"
                     /translation="MLTALSIRDVVLIERLDVSFSGGLTVLTGETGAGKSILLDSLGL
                     ALGARADAGLVRTGAEQASVTACFAPPPGHPLVALLTEQGLDADDEVILRRVVGRDGR
                     SRAFINDQAVAVTLLRHAAALLVEVQGQHEQIGLADQSSHAGLLDAFGVPRQLRDEIA
                     RAWRNWREAVVSLATAREAIAAAQRDEEWLRHAVTELAELAPASDEEEHLAQERQRLQ
                     QGERHAEAIAAALSELTPQDRRHPGPAAALRAASRALQRLVTPAQPNAVNPAAPALAS
                     LERAEEALAEAESFLTRLAHESDADPRSLEQAEERLFALRAAARKHAVMVAELPGLLD
                     TLSARLAALETGEAEVAGLEQAVRDARDAYVEAAAALGTARRSAATRLDRAVARELPP
                     LRLDKARFKAEMVALEEGEWGPAGTDAVRFLIATNPGQELGPVGRIASGGELSRLMLA
                     LKVVLSAGSPAPTLVFDEVDADVGGATAAAVGERLARVAERLQVLVVTHSPQVAARGS
                     AHLRVSKHAARGKTATRIEPLDAGARREEIARMLAGETVTEAARAAADDLLGAAA"
     gene            2590..4614
                     /gene="ligA"
                     /locus_tag="VKQ27_18930"
     CDS             2590..4614
                     /gene="ligA"
                     /locus_tag="VKQ27_18930"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLY91065.1"
                     /translation="MERLAHEIAHHDILYHQQDAPEVTDAEYDALRRRNAELEARFPH
                     LIREDSPSSRVGSAPESGFAKLRHRVPMLSLDNAMDAEDFREFCARARRFLGRPVDDR
                     LAFVAEPKIDGLSINLTYEHGRFVRGATRGNGAEGEDVTPNLRTIRTVPEKLKGRAPA
                     LIEIRGEVFMTKADFLKMNDAQAAAGHKVFANPRNAAAGSLRQLDPKITAERPLSLFA
                     YAQGESSEPVADTHWHYLQRLGEWGFQVNPLSRHLKSEDEAADFQANIALERSALGYD
                     IDGVVYKIDDLALQTRLGFVGRAPRWAIAWKFPAEQAMTVLRGIDIQVGRTGALTPVA
                     RLEPVNVGGVLVQNATLHNEDEIARKDIRIGDTVVLQRAGDVIPQIVSVIAEQRPKGS
                     KPYHFPEVCPVCGSHAVRPPSEAVRRCTGGLICEAQRVERLIHFVSRAAFDIEGLGEK
                     TIREFYEEGWLHSPADVFRLPAREAEIAGREGWGKISARNLSRAIEARRQIPLERFIY
                     ALGIRRIGESNARLLARHYGGFASWRADMLAAVEAGSEARGELDNIVGIGPAIAEELA
                     DFFGEPRNVHIVDELAAELTIEGAARIAAIDSEIAGKSLVFTGSLETMTRPEAKARAE
                     ALGAKVTDSVSKKTDLVVIGADAGSKARKATQLGVRTITEAEWRELAGLS"
     gene            4700..4936
                     /locus_tag="VKQ27_18935"
     CDS             4700..4936
                     /locus_tag="VKQ27_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91066.1"
                     /translation="MSGSRSIWDQPRRCARHHQALFVDYLLVAVLLTVMTVIEVQFLC
                     RVAGPDSVSLIQAADCIVRSDQIDTGGNGILARL"
     gene            5025..6020
                     /locus_tag="VKQ27_18940"
     CDS             5025..6020
                     /locus_tag="VKQ27_18940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLY91067.1"
                     /translation="MKRVAVIQPLPGIGDMIWHLPHIRAVAQWIGRPVTLIAKPRSAA
                     DELFASEQTIEDVLWLDRNPERRRGGHDGPGGLLRLSRSLRERRFQAAVLLHHSRTLA
                     AALFAAGIPHRYGYGYGLDRLFLNRPPYLPPATFAQHPHEQATDWVKAAGIPLEQSEP
                     SLSISEAHRAAARQKLETGTETFVVFGIGTSEPYKQWGLERYVELAGALLDAGCPRIV
                     LAAGTAESELADAIRQRAGDRISIAIGWKLGELAALLAEAAYYVGNDTGVMNLAAAAG
                     IRTYGLFGAVPPFHHSSAIVPILPPDGRTDKATGMARITTEAVLEAMRADRSRFS"
     gene            complement(6023..6739)
                     /locus_tag="VKQ27_18945"
     CDS             complement(6023..6739)
                     /locus_tag="VKQ27_18945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY91068.1"
                     /translation="MSFALKRRLAAAWLHDRRQFSRPWLARTCPICGYTGIFISVGHP
                     PRWDARCPQCGSRERHRLMYLWVHEGGGDRLAGKRILHFAPEKAVARHMQGNSLYETA
                     DLHQPGVTHQVDITRTGLPDAAYDVVIANHVLEHIDDDRAAMRELFRLLKPGGVAVLS
                     VPLNASREQTYENAGVQGMAERFAHFSAEDHKRYYGLDFTDRLAGVGFTTAIFRLPPE
                     DEVHYSLQRDEWLTIATKPR"
     gene            6818..7318
                     /locus_tag="VKQ27_18950"
     CDS             6818..7318
                     /locus_tag="VKQ27_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91069.1"
                     /translation="MAGITLSADEIKAAPPEIRRWLEHEVLRALGVVPATPAAPPPLV
                     GCNAEEARDMLALVQRMPPVVRVFWELAREGASMAVHGMRAFRIADIQRHVALAEPQQ
                     VIECLDVLTSALRRIRGDLEAAFYAVDDQGHCLVAEATMRSIIRLWQEIMAHGEIRQA
                     DDVGLT"
     gene            7375..7938
                     /locus_tag="VKQ27_18955"
     CDS             7375..7938
                     /locus_tag="VKQ27_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HLY91070.1"
                     /translation="MSETELLTRTPDQILGPFYPVRRTPVETGDLTMGGAASGTVLYL
                     SGRVLTTAGAPVAGARVEIWQANGKGRYDHPHDSNTAPLDPNFAGFAVTTTDAGGHYA
                     FKTIRPAAYPTGPDRMRPAHIHFSVTGVNEQLVTQMYFKGDPWNEKDTWLNSARRKDA
                     LIVDPVPAQGKEPGAQQVTFDIVLMRG"
     gene            complement(7956..>8240)
                     /locus_tag="VKQ27_18960"
     CDS             complement(7956..>8240)
                     /locus_tag="VKQ27_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLY91071.1"
                     /translation="RRLRPILMTSLAFILGVAPLVVATGAGAEMRQSLGTAVFGGMLG
                     VTGFGLLFTPAFFTLTQRFGRLPGRASAGGWAQQRGHEGAGVPVRSSAGD"
BASE COUNT         1514 a         2485 c         2842 g         1399 t
ORIGIN      
        1 catctgtctg ccgctgctcg tgagctgcgg ctccaaggat gagaatgcgg acgccgacaa
       61 gccggtggcg ccggttgaag acctgtacaa caacggtgtc gacgcgctga actcgcagcg
      121 atacgactcc gcgaacgatc agttcaatct tgttgaacag aactacccct actcgacctg
      181 ggcagtgaat gcccagctca tgcaaggcta tacgcaatac ttgcaaaaca aatacactga
      241 cgcgatcgga acgctcgacc ggttcatcca gttacatccg acgcatcgcg acgtcgccta
      301 tgcatattat ttgcgagcac tttgctatta tgagcagatt gcagacatcc agcgtgatca
      361 gcgcggcacg cagcttgcga tgaatgcgtt gcaggaagtc gtgaaccgct tcccggacag
      421 tgcttatgcc agagatgcgc ggctcaagat cgacctttgt aacgaccatc tcgccggcaa
      481 ggagatggat atcggccgtt ggtatgaaag ccagcatctc tacaccgcgg ctatcggccg
      541 atttcagcgt gtcgtggacg actatcagac gaccaatcac gtgcccgagg cgctgcacag
      601 gttaactgag atctatctga tccttggtct gaaggaccag gctcgaaaga ccgcttcagt
      661 gctgggctac aactatcctg gcagttcctg gtacgaagac agctacaatg acctgttcga
      721 cgctggcctc gtcaatcagc agggcccaac gccggcaccg cagcgcggct tcatttcccg
      781 cgcgtggcat tcgttgttct gagccggcgc ggcagcaagc ggcagccgca tgcttactgc
      841 tctgtccatt cgcgacgtgg tactgatcga gcggctggac gtgtcgtttt ccggcggcct
      901 gacggtgctg accggcgaga cgggtgccgg caaatccatc cttctggata gcctggggct
      961 ggcgctcggc gcccgggcgg atgccggact ggtgcggact ggcgctgaac aggccagcgt
     1021 tacggcgtgc ttcgcgccac cgcctgggca cccgctggtc gcgttgctta ccgagcaggg
     1081 gctggacgcc gatgacgaag tgatcctgcg ccgcgtggtg ggccgtgacg gccgctcgcg
     1141 cgcgttcatc aatgaccagg ccgttgcggt gacgctgctg cgccacgcgg ccgctctact
     1201 ggtagaggtc caaggccagc acgagcagat cgggctcgcc gatcagtcta gccacgctgg
     1261 gctgttggac gcattcggag tgccgcggca attgcgggat gagatagcgc gagcgtggcg
     1321 gaactggcgc gaagcggtgg tgtcgctggc gacggcgcgc gaagcgattg cggcggcaca
     1381 gcgcgatgag gaatggcttc ggcatgccgt gaccgaactt gctgaactcg caccggcgtc
     1441 cgatgaggag gaacatctgg cacaggagcg gcagcgcctg cagcagggcg agcgccacgc
     1501 cgaggcgatc gccgcagcct tgtccgaact gactccgcag gatcggcggc atcccggccc
     1561 tgccgcggcg ttgcgggccg caagccgggc attgcagcgc ctggtaacgc cagctcagcc
     1621 aaatgcagtc aatccggcgg cccctgccct cgcctctctc gaacgggcgg aagaggcgct
     1681 cgcggaggct gagagcttcc ttacgcgtct cgcgcatgaa tccgacgccg atccgcgctc
     1741 attggagcag gcggaggaac ggctgtttgc cctgcgtgcc gcggctcgca agcatgccgt
     1801 gatggtggcc gaactgccag gattgctgga cacgctgtcg gcccgattgg cggcgctgga
     1861 aaccggcgag gcggaggtgg ccggattgga gcaggcggtg cgcgatgccc gcgatgccta
     1921 cgtcgaggcg gcggcggccc tggggacggc aaggcggtcg gcggcgaccc ggctggatcg
     1981 cgccgtggcg cgagaactgc cgccgttgcg gctggacaag gcgcgcttca aagcggagat
     2041 ggtcgctctg gaagaaggcg aatgggggcc tgccggcacc gacgcggttc gcttcctgat
     2101 cgcgaccaac ccgggtcagg aactaggacc ggtggggcgg atcgcctcgg gcggtgagct
     2161 gtcgcgactg atgctggcac tgaaggttgt cttgtcggcc gggtcacctg caccgactct
     2221 ggtgttcgat gaggtcgatg cggatgtcgg tggcgcaaca gcagcggcgg tcggagaacg
     2281 gctggcgcgc gtggcggagc ggttgcaggt gcttgtggtg acgcacagtc cgcaggtggc
     2341 agcacgcggc agcgcgcatc tgcgcgtgtc gaagcacgct gcgcgcggga aaacggctac
     2401 gcggattgag ccgctggacg caggtgcccg tcgcgaggag attgcgcgca tgctcgccgg
     2461 cgagacggtg accgaggcgg cgcgcgcggc ggcggacgat ttgctgggag cagcggcgtg
     2521 agccgcgccc cgaaggcacc ggatgcggga tctgtcggcg cgctgacaga aagggaggca
     2581 gccgccgaac tggagcggct ggcgcacgaa atcgcgcatc atgacatcct gtaccatcag
     2641 caggatgcgc cggaagtcac cgacgccgaa tacgacgcgc tccgtcgaag gaacgccgaa
     2701 ctcgaggcgc gctttcctca tctgatccgc gaggattcgc cttccagtcg cgtgggcagc
     2761 gccccggaaa gcggattcgc caagctgcgg catcgcgtcc cgatgctgtc gctcgacaac
     2821 gcgatggatg ccgaggattt ccgtgaattc tgcgcacgcg cgcggcggtt tcttgggcgc
     2881 ccggttgacg atcggctggc attcgttgcc gaaccgaaga tcgacgggct gtcgatcaac
     2941 ctgacctatg agcacggccg gttcgtgcgc ggcgcgacac gcggcaacgg tgccgagggt
     3001 gaggatgtca ccccgaatct ccgcaccatc cgcaccgtgc cggagaagct gaaaggccgc
     3061 gcgccggcgc tgatagagat tcgcggcgaa gtgttcatga ccaaggccga cttcctgaaa
     3121 atgaacgacg cgcaagcggc cgctggacac aaggtgttcg ccaatccgcg caacgcggcg
     3181 gcgggaagcc tgcggcaact cgatccgaag atcactgccg aacggccgct gtcactgttc
     3241 gcgtatgcac agggagagtc gagcgaaccg gttgccgata cgcattggca ttacctgcaa
     3301 cggctaggtg agtggggctt ccaggtaaac ccgctatcgc ggcatctgaa aagcgaagat
     3361 gaggccgccg acttccaagc caacatcgcg ctggagcgat cggcgttggg ctacgacatc
     3421 gatggcgtgg tctacaagat cgatgacctg gcattgcaga cgcgccttgg gttcgtcgga
     3481 cgtgcgccgc gctgggcgat tgcttggaag ttccctgccg agcaggcgat gaccgtgctg
     3541 cgtggcatcg atatccaggt tggccgcact ggtgcattga cgccggtcgc acggctggag
     3601 ccagttaatg tcggcggcgt gctggtgcag aatgcgacat tgcacaatga ggacgagatc
     3661 gcgcggaagg acattcgtat cggcgacacg gtggtgctcc aacgtgccgg cgacgtgatt
     3721 ccgcagatcg tttcagtgat agccgaacag cggccgaagg gaagcaagcc gtatcacttc
     3781 cccgaagtct gtccggtctg tggcagccac gcggtacgtc cgcccagcga ggcggtgcgg
     3841 cgctgtacag ggggcctgat ctgcgaggcc caacgagtcg aacggctgat ccactttgtg
     3901 tctcgagccg ccttcgacat cgaaggactg ggcgaaaaga ctatccgcga gttttacgag
     3961 gagggctggc tgcatagtcc cgccgacgtc ttccgactgc cggcacgcga ggcggagatc
     4021 gcgggccgcg agggatgggg caaaatctcg gcgcggaacc tgtcgcgcgc aatcgaggcg
     4081 cgacggcaga ttccgctgga gcggttcatc tatgcgctgg ggattcgtcg gatcggcgaa
     4141 tcgaatgcgc ggctgctggc acggcattac ggcggctttg ccagttggcg cgcggacatg
     4201 cttgcagcgg tggaagcagg ctccgaggcg cgcggcgaac tcgacaacat tgtgggcatc
     4261 gggccggcga tcgccgagga gttggcggac ttcttcggcg agcctcgcaa cgtgcatatt
     4321 gtggatgagc tggcggcgga gcttaccatc gagggcgctg cacgcatcgc cgcgattgac
     4381 agcgaaattg ccggtaagag cctggtgttc accggcagtc tggagacgat gacgcgccca
     4441 gaagcgaagg cacgtgcgga ggcgttgggg gcgaaagtca cggacagcgt ctcgaagaag
     4501 accgatctgg tggtgattgg tgcagacgcg gggtcgaagg cgcgcaaggc gacccagctc
     4561 ggtgtccgca ccatcactga ggcggaatgg cgagaacttg cgggactgag ctaagcgcca
     4621 acctgtgtga tccagttcat accgtcgatt tgtaggattg cgcgattgtc cgctgcgtca
     4681 tgtcatggag acgcagcaga tgagcggctc gcgcagcatc tgggatcagc cgagacggtg
     4741 cgccaggcat catcaggctc tgttcgtcga ctacttgctc gtcgccgtgc tcctgaccgt
     4801 catgacagtg atcgaagtgc agttcctgtg ccgggtcgcc ggaccggata gtgtgagcct
     4861 gatccaggcg gcggactgta tcgtcaggtc cgatcagatc gacaccggcg gcaacgggat
     4921 cctggcgagg ctctgatcgc gaagctgttt gacggcatac cggctcccag ctacgtgggt
     4981 cagtcaggag attttgccca agcggctgaa tgtttgatcg gacattgaag cgtgtcgccg
     5041 tgatccagcc gttgcctggt atcggcgaca tgatctggca tcttccgcat atccgcgccg
     5101 tcgcgcagtg gattggtcgg ccggtgacat tgatcgcgaa gccacgttcg gcggccgacg
     5161 agctgtttgc gtccgagcag acgatcgagg acgtgctgtg gctggatcgg aatccggaac
     5221 gccggcgtgg cggccacgat ggccctggcg gtttgctgcg cctgagccgt tcgttgcggg
     5281 aacgcaggtt ccaggccgcg gtactgctgc atcacagcag gacgctggcc gcggcgctgt
     5341 tcgcggccgg aataccgcac cgctatggct acggttatgg gcttgaccgg ctgtttctga
     5401 acaggccgcc ttacctgcca ccggcgacct tcgcgcagca tccccacgaa caggccacgg
     5461 actgggtgaa ggcggcgggg attccgctgg aacagtcaga gccgtctctc agtatcagcg
     5521 aggcacaccg agccgccgcg cgacaaaagc tggagacagg gactgagacg ttcgtcgtct
     5581 ttggcatcgg tacgtcggag ccatacaagc aatggggact agaacggtat gtggagcttg
     5641 ccggcgcact gctcgatgca ggatgcccac gtatcgtgct ggctgctggc acggcggaga
     5701 gcgaactggc tgatgcgatt cgccagcggg ctggagaccg gatcagcatc gcgatcggct
     5761 ggaagctggg tgaactggcc gcactgctgg ccgaagccgc atattatgtt gggaacgata
     5821 ccggcgtgat gaacttggca gcggcggctg ggatcaggac ctatgggcta tttggcgcgg
     5881 tgccgccgtt tcatcatagt agcgcaattg tgccgattct cccgcctgat ggacgtacgg
     5941 ataaggccac gggaatggcg cgcatcacaa ccgaggcggt actggaagcg atgcgagccg
     6001 accgcagccg tttttcgtga aactacctgg gttttgtggc gatagtcagc cactcatcgc
     6061 gctgcagact gtagtgcacc tcgtcttcag gcggcagacg gaatatcgcg gtggtgaagc
     6121 cgacgccggc gaggcgatcg gtgaagtcga gaccgtagta gcgcttgtgg tcctcggcgc
     6181 tgaaatgcgc gaaccgctcg gccatgccct gcacgccggc attctcgtaa gtctgctcac
     6241 gcgacgcgtt cagcggcaca ctcagcaccg cgacaccgcc tggcttcagc agccggaaca
     6301 gctcgcgcat cgcggcgcgg tcatcatcga tatgctccag cacgtggttg gcgatcacca
     6361 cgtcgtatgc ggcgtctggc aggccggtgc gcgtgatgtc cacctgatgc gtgacacccg
     6421 gctggtgcaa atcggcggtc tcgtacagcg aattgccctg catgtgccgc gccaccgcct
     6481 tttctggcgc gaaatgcagg atacgcttgc cggcgagccg atcgccaccg ccttcatgga
     6541 cccagagata catcaggcgg tggcgctcac ggctcccgca ctgcgggcag cgcgcatccc
     6601 agcgtggcgg atgaccgaca ctgatgaaga tcccagtgta gccgcagatc ggacaggtac
     6661 gcgccagcca agggcgggag aactgccgtc ggtcgtgcag ccaggccgcg gcgagacggc
     6721 gcttcagcgc aaagctcatg cttgatcggg atcaatgcgg cggagcggca aacccgccat
     6781 gacgctggcc ttcaacctga caaatgggag agcaggtatg gccggtatca cgctgtcggc
     6841 ggacgaaatc aaggccgcgc cgcccgagat tcgccgctgg ctggaacatg aggtgctgcg
     6901 cgccctcggg gtagtgccgg ccacacctgc tgcaccgcca ccgctggtcg gctgcaatgc
     6961 cgaggaggca cgcgacatgc tcgccctggt gcagcgaatg ccaccggtgg tgcgcgtgtt
     7021 ctgggaactg gctcgcgaag gcgccagtat ggcggtgcac ggcatgcgtg cgttccggat
     7081 cgccgatatc cagcgccacg tggccttggc cgagccgcag caggtcatcg aatgcctgga
     7141 cgtgctgaca agcgcgctca ggcggatccg cggcgacctg gaggcggcat tttatgccgt
     7201 ggacgaccag gggcactgcc tggtggcaga ggcgacgatg cgcagcatca tccgcctctg
     7261 gcaggagatc atggcgcatg gcgagatccg tcaggcggac gacgtgggcc tgacataggc
     7321 tgcttgcgcg gacgggcacg gcggcgcgat catgtcgggc aggagggacc cgccatgtcc
     7381 gaaacggaac tgctgacccg cacgcctgac cagattctcg ggccgttcta tccggtgcgc
     7441 cgcacccccg tcgagaccgg cgacctgacg atgggcggcg cggccagcgg gacggtgctg
     7501 tatctcagcg gccgggtgct gacgacggcc ggagcgccgg tggcaggggc gcgggtagaa
     7561 atctggcagg cgaacggcaa gggccgctac gaccatccgc acgactccaa caccgcgccg
     7621 ctcgacccga atttcgccgg gttcgcggtg accacgaccg atgcgggcgg acactacgca
     7681 tttaagacaa tccggccagc ggcgtatccg actggtccag accggatgcg gccggcgcat
     7741 atccacttca gcgtgaccgg cgtgaatgag cagctggtga cgcagatgta cttcaagggc
     7801 gatccctgga acgagaagga cacctggctg aacagtgccc ggcggaagga cgcgctgatc
     7861 gtcgatccgg tcccggccca gggcaaggaa ccaggcgcgc agcaggtgac gttcgatatc
     7921 gtgcttatgc gcgggtaggt cctccccctc gccgctcagt ctcccgcgga cgaccgcact
     7981 gggacgccgg ccccttcgtg gcctctctgc tgcgcccatc ccccggctga ggcgcggccg
     8041 ggtaacctcc cgaaccgctg tgtcagggtg aagaacgccg gcgtaaacag caggccgaag
     8101 ccggtcaccc cgagcatgcc gccgaacact gcggtgccga gcgactgacg catctcggcg
     8161 cccgcgccgg ttgccaccac caggggagcg acgccaagga tgaaggcaag cgatgtcatc
     8221 aggatcggcc gcaacctacg
//
LOCUS       DATGEM010000614         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1595595, whole genome shotgun sequence.
ACCESSION   DATGEM010000614 DATGEM010000000
VERSION     DATGEM010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595595"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VKQ27_18965"
     CDS             complement(<1..368)
                     /locus_tag="VKQ27_18965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91072.1"
                     /translation="MAASPISARMRRMQWIAIGLATAAIALNYVDRSTLAIGNLKIRE
                     EFGINATAIGALQSAWSITYALAQVPIGYFLDRLGPRWLVGVALILWSMAQAAGGFAL
                     SYVQLLWARVALGAAECPAFP"
     gene            480..1151
                     /locus_tag="VKQ27_18970"
     CDS             480..1151
                     /locus_tag="VKQ27_18970"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HLY91073.1"
                     /translation="MSDSAAGLRIDIVSDAICPWCYIGKRQLERALATLAHEGLHFSV
                     HWNPFQLNPDMPKEGRDRASYRMMKFGSADRVREIDERVTGAAANVGLEFRLDQIVRT
                     PNTLDAHRLIWRAGREDRQDAVVEALFTAYFTHGRDIGDRDVLADCAAEAGMDRAAVA
                     VFLAGDVAAKEMLAADRAAREAGVNGVPSFFLDGYGLYSGAMPAETMAEALRKGNQIL
                     RQRAA"
     gene            1213..2532
                     /locus_tag="VKQ27_18975"
     CDS             1213..2532
                     /locus_tag="VKQ27_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HLY91074.1"
                     /translation="MSNETKHPETLVLHAGWRRDPATNAVAVPIYQTTSYQFDSAEHA
                     ANLFGLRELGNIYTRIMNPTQDAFEKRIAAVEGGVAALGLASGQAASAFSVQNLARAG
                     DNIVSSTDLYGGTWNLFANTLKDQGIEARFVDPSDPEAFARATDDRTRAYYAETLPNP
                     KLVAFPIAEVAAIGRKFGIPLIMDNTAAPTLVRPFDHGAAVVMYSTTKYIGGHGTSIG
                     GVVIDGGNFPWDSHKERQPLLNTPDPSYHGAVWAEAAKPLGPIAYILRMRVVLLRDLG
                     AAMSPFNAFLFLQGLESMPLRMREHCKNTLAVAQYLSSRKEVTRVIHPAFHKGVGAER
                     TAKYLPHGHGGLLGFELAGGVEAGRAFINALQMFYHVANIGDSRSLAIHPASTTHSQL
                     TPEEQLATGVTPGYIRLSIGIEHIDDIIGDLSQALDAAGVGLRAAAE"
     gene            complement(2513..2971)
                     /locus_tag="VKQ27_18980"
     CDS             complement(2513..2971)
                     /locus_tag="VKQ27_18980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HLY91075.1"
                     /translation="MTARVVVGIGNPDRGDDAVGRIVASLVQSSVMPDIPVTLLSGEG
                     AGIMAALEGVDEAWLVDAARSGASPGTIHRIDCAMDKVPPCGSVSSHDFGLAEAIELA
                     RALGTLPPQCILYAIEAADFTLGAPLSPDVVAAAHTVAQRIRQELIRPPP"
BASE COUNT          587 a          994 c         1004 g          506 t
ORIGIN      
        1 gggaatgccg gacattccgc ggcgccaagc gcgacgcgtg cccagagcaa ttgcacatag
       61 gacagggcaa agccgccggc tgcttgcgcc attgaccaca ggatcagcgc gacaccgacc
      121 agccagcgtg gccccagccg atcgaggaaa tagccaatcg gcacctgggc caatgcatag
      181 gtgatcgacc aggccgattg cagcgcgcca atcgcagtcg cgttgatgcc gaattcctcg
      241 cggatcttca ggttgccgat ggcgagtgtg gaacgatcca cataattcag ggcaatcgca
      301 gcggtggcta atccgattgc gatccactgc atacggcgca tgcgcgctga gatcggcgag
      361 gcggccatgg tctcctccag tcgtttcttc tgtttggaat tattggctca cgccgtgccg
      421 agattggcaa gcaccggcat cgcgcccatg ttatgcagta acacacagga gcgagagtga
      481 tgagcgattc cgctgccgga ctacgtatcg atatcgtatc tgacgcaatc tgtccgtggt
      541 gctatatcgg caagcggcag ctggagcgcg cgctggcgac gctggcgcac gagggcctgc
      601 atttctcggt gcattggaac ccgttccagc tcaatcctga catgccgaag gaaggtcggg
      661 accgcgccag ctaccgcatg atgaaattcg gcagcgccga ccgcgtgcgc gagatcgacg
      721 agcgggtgac gggcgcggct gccaatgtcg gtctggaatt ccgtctggac cagatagtgc
      781 gcacgcccaa tacgctggat gcgcaccggc tgatctggcg cgcaggccgg gaggaccggc
      841 aggacgcggt ggtcgaggcg ttgttcacag cctatttcac ccacggccgc gacatcggcg
      901 accgcgacgt gctggccgat tgtgcggccg aggcgggcat ggatcgcgcc gcggtggcgg
      961 ttttcctggc gggcgacgtg gccgcgaaag aaatgctcgc agccgaccgc gcggcgcggg
     1021 aagcgggcgt gaacggtgtg ccgtcgttct tcctggacgg ctacggcctc tattccggcg
     1081 cgatgccagc agaaaccatg gccgaggcgc tgcggaaagg aaatcaaatt ctccgccaac
     1141 gcgccgcttg acattgcggc tgcccccggg gcttggtccg ctctgacaca gacatcaggc
     1201 ggggagcgag ccatgtcgaa cgagacgaag catccggaaa cgctggtgct gcacgccggt
     1261 tggcggcgtg atccggccac caatgccgtc gccgtgccga tctaccagac cacatcgtat
     1321 cagttcgaca gcgccgagca cgctgcgaac ctgttcggac tgcgcgagct gggaaacatc
     1381 tatacgcgca tcatgaaccc aacacaggat gcgttcgaga agcgcattgc ggcggtggaa
     1441 ggcggcgtgg ccgcgctcgg gctggcgtcc ggccaggcgg catcggcgtt ttcagtgcag
     1501 aacctggcac gggccggcga caacatcgtg agttccacgg acctgtacgg cggcacctgg
     1561 aacctgttcg ccaacacgct gaaggaccag ggtatcgaag cccgtttcgt ggatccctcc
     1621 gaccccgaag cgttcgcgcg cgccacggat gaccgcacgc gcgcctacta tgccgagaca
     1681 ctgccgaacc cgaagctggt cgcgttcccg atcgcggaag tagcagcgat tggccgcaag
     1741 ttcggcatcc cgttgatcat ggacaacacg gcggcgccca ccctggtgcg gcccttcgac
     1801 catggcgcgg cggtggtgat gtactccacc acgaaataca tcggcggcca cggcacctcg
     1861 atcggcggcg tggtgatcga tggcggcaac ttcccgtggg actcacacaa ggaacgccag
     1921 ccgctgctga acacgcccga tccgagctac cacggcgcgg tgtgggccga ggctgcgaag
     1981 ccgctcgggc cgatcgcgta tatactgcgg atgcgcgtgg tgctgctgcg tgacctgggc
     2041 gcggcgatga gcccgttcaa tgcgttcctg ttcctgcaag ggctcgagag catgcctctg
     2101 cgcatgcgcg agcactgcaa gaacacgctt gccgtggcgc agtacctgtc cagccgcaag
     2161 gaagtgacgc gggtgatcca tccggcgttc cacaagggcg tcggcgcgga acgcacggcg
     2221 aagtacctgc cgcacgggca cggcggcctg ctcgggttcg agcttgcggg cggcgtggag
     2281 gcagggcgcg cgttcatcaa cgcgctgcag atgttctacc atgtggcgaa tatcggcgat
     2341 tcgcgttcgc tggcgatcca ccctgcgagc acgactcact cgcagcttac acccgaggag
     2401 cagcttgcaa ccggcgtgac accggggtat atacgcctgt ccatcggcat cgagcacatc
     2461 gatgacatca tcggcgatct gtcacaggcg ctggacgcgg ccggcgtcgg cctcagggcg
     2521 gcggccgaat aagctcctga cggatccgtt gcgccacggt atgtgccgct gccactacgt
     2581 caggtgacag cggcgcaccc agggtgaagt cggccgcctc gatggcgtag aggatgcatt
     2641 gcggcggcag cgtgccgagc gcgcgggcaa gctcaatcgc ctcggcgagc ccgaagtcat
     2701 gcgaggagac gctgccgcac ggcgggacct tgtccatcgc gcagtctata cgatgaatcg
     2761 ttcccggcga tgcacctgac cgggcggcat caaccagcca cgcctcatcc acaccctcca
     2821 gcgcagccat gatcccggcg ccttcgccgc tgagcagtgt gacaggaatg tctggcatga
     2881 cactcgattg caccagcgaa gccacgatgc gccccactgc atcatcgcca cgatcagggt
     2941 tgccaatacc caccacgacc cgtgcggtca catacgcacc acttccagct tcaggaaatg
     3001 cgtcgcgcag gagatgcaag gatcgtgatt gcgcaccgcc tgctcacagc gcacgcgcag
     3061 cgcctcctcc ggcaggtcca gccaatcacc c
//
LOCUS       DATGEM010000615         3485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_383763, whole genome shotgun sequence.
ACCESSION   DATGEM010000615 DATGEM010000000
VERSION     DATGEM010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383763"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VKQ27_18985"
     CDS             complement(<1..615)
                     /locus_tag="VKQ27_18985"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLY91076.1"
                     /translation="MSVDLIIEGPVAKIILNRPEKLNALTYPMRAQLRDYTQQLRFDD
                     NVRAIIVTGEGRAFCTGADVGGMQRGGLRAERQKMQGGSHQFLRNLHAIEKPVIAAVR
                     GPTVGIGWSLALACDLIVASETARFSQVFRRIGLAPDGGAIWFLTRRIGMARAKELVF
                     SARFVEAPEALELGLVNHVVPDGELMAKTEELAADLATGPTFAFG"
     gene            712..1899
                     /locus_tag="VKQ27_18990"
     CDS             712..1899
                     /locus_tag="VKQ27_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLY91077.1"
                     /translation="MARPFEGIRIIDITHVLAGPFAAYQLGLMGADVIKVEHPDDPDQ
                     SRDSGSDAALNHAGMGTGFLTQGSNKRAITLNLKTEQGREILKKLVATADVLVENYRP
                     GAFEALGLGYEEMRKINPKLIYASFSAFGQGGPRREQTAYDHVIQSTSGIMAMTGTPA
                     ANPVKIGAPAIDYSTGTMGAFALSAALFQRERTGQGQRIDMAMLDVAMILMASHVTGF
                     MRNGHHPKPSGNRMPHATSMAYQTKDGMVMLGASNLRQQKRLWTVLEHPEMVKRSNDE
                     RLADHEREEALLAGIMLTRSAAEWEAWLQERHVPASRVRTMGEALADPQIASRGVLHR
                     HEGVAGIEGAFTVPVAAFKFAHDGPRVDRAPPRFGEHTDEVLGELGYSGADIAGFRKA
                     GVV"
     gene            2039..2902
                     /locus_tag="VKQ27_18995"
     CDS             2039..2902
                     /locus_tag="VKQ27_18995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY91078.1"
                     /translation="MQLGRLGAWYATDKLGGPAQIKSFVQTVERLGYDTLWYPESRGY
                     ESLSVAGFMLQHTTKLKMGSSIASIYARDAFTARRGLLSLQALYGDRFILGLGVSHVP
                     MVEGIRGHTYQKPVPAMRSYLEGITKGEAGSDGWPIAIAALGPLMLKLSATHTRGALP
                     YNTTPQHTAEAARIIGPGKWLVAEQKVTLETDPAKARALGRKELSRYMVLDNYRNNWL
                     RIGFTEAELANGGSDRFIDAMCLWGSADKVREGLRAHFAAGATHVCIQPVHEDGDTAA
                     RDRILTELANT"
     gene            2910..>3485
                     /locus_tag="VKQ27_19000"
     CDS             2910..>3485
                     /locus_tag="VKQ27_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HLY91079.1"
                     /translation="MLAIPKSGPISRTTMAYVLAGGRGSRLMELTDRRAKPAVFFGCK
                     SRIIDFALSNALNSGIRRIGVATQYKAHSLIRHLQRGWNFFRVERNESFDILPASQRV
                     SETAWYEGTADAVYQNIDIIESYAPDHIIILAGDHIYKMDYEPMLQMHHERQADVTVG
                     CIEVPRSEASGFGVMQIDSTDRIVQFLEKPKD"
BASE COUNT          639 a          958 c         1256 g          632 t
ORIGIN      
        1 gccgaatgcg aaggttggac ccgtcgccag gtcggctgcc agctcttcgg tctttgccat
       61 cagttcgccg tccgggacga cgtggttcac caggccgagt tccaatgctt cgggggcctc
      121 gacgaagcgg gcggagaaga cgagttcctt ggcgcgcgcc atgccgatgc ggcgggtgag
      181 gaaccagatc gcaccgccat ccggcgccag gccgatacgg cggaacactt gtgagaaacg
      241 cgcagtctcg gatgcaacga tcaggtcaca ggcgagtgcc aggctccagc ctatgccgac
      301 ggtgggaccg cggacggcgg cgatcaccgg cttctctatc gcgtggagat tgcgcaggaa
      361 ctggtggctg ccgccttgca tcttctggcg ctcggcacgc aggccgccac gctgcatgcc
      421 gccgacatcg gcgccggtgc agaaggcgcg gccctcgccg gtgacgatga tggcgcggac
      481 attgtcatcg aagcgaagct gctgggtgta gtcacggagc tgcgcgcgca tcgggtaggt
      541 gagggcgttc aacttctccg gccggttcag gataatcttc gccaccgggc cctcgatgat
      601 caggtcaacc gacatcgcct gcgttcctcc gcgttccttc ggtccatcca agcgtgttga
      661 cgccatgcgg gcaaggcgcc accgttacgc ggtgcgttgg aggaggcggc tatggcgcga
      721 ccgttcgagg gcatccgcat aatcgacatc acgcacgtgt tggcgggtcc gttcgcggcc
      781 tatcagcttg ggctgatggg tgccgatgtc atcaaagtgg aacatccgga cgatcctgat
      841 cagagccgcg attccggaag cgatgccgcg ctgaatcatg cggggatggg aacggggttc
      901 ctgacgcagg gatccaacaa gcgtgcgatc acgttgaacc tgaagacgga gcaggggcgc
      961 gagatcctga agaagctggt cgcgacggcg gatgtgctgg tggagaatta ccggccgggc
     1021 gcgttcgagg cgctggggct tggctatgag gagatgcgga agatcaatcc gaagctgatc
     1081 tacgcgtcgt tctcggcgtt tgggcagggc gggccgcggc gcgagcagac cgcgtacgac
     1141 catgtgatcc aatcgacgtc gggcatcatg gcgatgacgg ggacgccggc ggcgaacccg
     1201 gtgaagatcg gtgcgccggc gatcgattac tcgaccggca cgatgggggc gttcgcgctg
     1261 tcggcggcgc tgtttcagcg ggagcgcacc gggcagggcc agcggatcga catggcgatg
     1321 ctggacgtgg cgatgatcct gatggcctcg catgtcacgg ggtttatgcg gaacgggcat
     1381 catccgaagc cttcgggcaa caggatgccg catgcgacga gcatggcgta tcagaccaaa
     1441 gatgggatgg tgatgctagg ggcgtccaat ctccggcagc agaaacggtt atggaccgtg
     1501 ctggagcatc ctgagatggt gaagcgctcc aatgacgagc ggctggcgga ccatgagcgc
     1561 gaagaggcgt tgctggccgg catcatgctg acgcgcagcg cggcggagtg ggaggcgtgg
     1621 ttgcaggagc ggcatgtacc ggcttcgcgc gtacggacga tgggcgaggc gctggcggat
     1681 ccgcagattg cctcgcgtgg ggtgctgcat cggcacgagg gagtggcggg gatagagggc
     1741 gcgttcacgg tgccggtggc ggcgttcaag tttgcgcatg atgggccgcg ggtggatcgg
     1801 gcgccgccgc gatttgggga gcataccgac gaggtgctgg gggagttggg atatagtggg
     1861 gcggatatag cgggatttcg gaaggcgggg gtggtttgac aattggccca atagtcatgc
     1921 cgggtgcagg tccggcaccc gcatcgtcgt tgctgatggg taggaaagac gtgggtgccg
     1981 acctttgtcg gcatgacggt tggggaaagc tcgcctagct gtttgaatgg agagattgat
     2041 gcaactcggt cgactgggcg cgtggtatgc gacggacaag ctgggcggtc ccgcacagat
     2101 caagtcattt gtgcagacgg tcgaacggct tggctacgac acgttatggt atcctgaatc
     2161 gcgcgggtac gagtcgctgt cggtggccgg gttcatgctt cagcacacga cgaagctgaa
     2221 gatgggcagt tccattgcgt cgatctatgc gcgcgacgcg ttcaccgcgc ggcgcgggtt
     2281 gctgtcgttg caggctctgt acggcgatcg gttcatcctg gggcttggtg tttcgcatgt
     2341 tccaatggtg gagggcatcc gcgggcacac ttaccagaag cctgtaccgg ctatgcgttc
     2401 ctacctggag ggcatcacca agggagaagc cggctccgat gggtggccga tcgcgatcgc
     2461 ggcgctgggg ccgctgatgc tgaagctgtc ggccacgcat acgcgaggag cgttgccgta
     2521 taacaccacg ccgcaacata ccgcggaggc ggcacgcatc attgggccgg gcaagtggct
     2581 ggtggccgag cagaaggtta cgctggaaac cgatccggcc aaggcgcggg cgctcgggcg
     2641 gaaggagctg tcgcggtaca tggtgctcga caactaccgg aataattggc tgcgtatcgg
     2701 gttcaccgag gctgagctgg caaacggcgg atcggaccgg ttcatcgatg cgatgtgcct
     2761 gtggggcagc gccgacaagg tgcgcgaagg gctgcgcgcg cactttgctg ctggtgccac
     2821 gcatgtctgt atccagccgg tgcacgagga tggcgacacc gccgcacgtg accgcatcct
     2881 gacggagctt gccaacacct gaggacccta tgctggccat cccgaaatcc ggaccgatct
     2941 cgcgtactac gatggcctat gttctggcgg gcgggcgcgg cagccgcctg atggagctga
     3001 ccgaccgccg ggcaaagccg gcggtgttct tcgggtgcaa atcgcgcatc atcgatttcg
     3061 cgctgtcgaa tgcgctgaat tcggggatca ggcgtatcgg cgtggcgacg caatacaagg
     3121 cacacagcct gatccggcac ctgcagcgcg gctggaactt cttccgggtg gagcgcaatg
     3181 agagcttcga catcctgccg gcgtcgcagc gggtctcgga gaccgcgtgg tacgaaggca
     3241 cggctgatgc ggtctatcag aacatcgaca tcatcgagag ctatgcgccg gaccacatca
     3301 tcattctggc gggtgatcat atctacaaga tggactacga accgatgttg cagatgcatc
     3361 acgagcgtca ggccgatgtg acggtcgggt gcatcgaagt gccgcgcagt gaagcctcgg
     3421 gctttggcgt catgcagata gattccaccg atcgcatcgt ccagttcctg gaaaagccga
     3481 aggac
//
LOCUS       DATGEM010000616         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_259856, whole genome shotgun sequence.
ACCESSION   DATGEM010000616 DATGEM010000000
VERSION     DATGEM010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259856"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VKQ27_19005"
     CDS             <1..1645
                     /locus_tag="VKQ27_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY91080.1"
                     /translation="VVGDTLNMIAYNISKSEIEWVGVRHEEAGAFAAQGEASLTGNLT
                     AVAGSCGPGSLHFINGIVEANRNRAPVILIASQIVRDELGFDFPQELDFKAIYGSCTV
                     YCDMILTPEQARRKTVMACQAAIAKRGAAVLIVPVDISHSDVQDDVPFAVQTSHPVVR
                     PTDDDLDEIAGIINSGEQIAIYGGSGCRGAHDEILRVAEILKAPIAHTSRGKDSLEYD
                     NPYNVGMTGIIGNEAAYRAVRECDTLLLLGADFAWRQFYPKHAKIVQIDIDPAHLGRR
                     HPVTRGLVGDIKATLEALIPRLHEKMDTDFRDRFVKRHRDVIASQKEKAAPKRRDTIS
                     GIHLTELIDRMAADNALVAGDDGTPTVWMHRYFTATRERRFYASLLHGTMAAAMPTAL
                     GLQKAAPGRQVIALSGDGGIGMLFGDLMTAVQEKLPIKVAVFDNGKLGFIDIEQKSEG
                     MLPLYTHLQNPDFGAVAEAMGLWGRTVAKAGEIEAAIGEWLAHPGPALLNVKVESTTL
                     VMPPAIEFGPAYGMALYSARAVLGGQTQELMEMIQENFL"
     gene            complement(2019..2378)
                     /locus_tag="VKQ27_19010"
     CDS             complement(2019..2378)
                     /locus_tag="VKQ27_19010"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HLY91081.1"
                     /translation="MDEAEAAGLRFFFRIADRWNLTESEQLKILGLEAKPELDALRSN
                     EAPQLAAGTLERVSYALGIYKAINTLLPIPERADNWMRATNNAPTFAGMTPAARMAGG
                     TIADLQAVRRYLEAQLV"
     gene            complement(2430..3020)
                     /locus_tag="VKQ27_19015"
     CDS             complement(2430..3020)
                     /locus_tag="VKQ27_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91082.1"
                     /translation="MWISPTAALRMTEDRTPPGVESFRTGVARASLGAAASSLAQAKL
                     QPRCPTDGDRINPHLWPITAADWKVVLAPRVPDAFQPPELLFPAFDRHHLPEEDVVGL
                     VTTVYYPKAITRHDCMAEVIAYADRLACRWALSSLVVLHVPGDSRHEMAMHGHILSLP
                     LKHLPTGFAAPTPLLNKKGPGLLHKELLDLRAARCR"
     gene            complement(3024..3482)
                     /locus_tag="VKQ27_19020"
     CDS             complement(3024..3482)
                     /locus_tag="VKQ27_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91083.1"
                     /translation="MMLAGQPFHGCPEAARSSARSYLFAPDLDALIVPWTVVTDRAVG
                     EVEAEVMRIGAPSLIVHPIGIGAFAFRVGIAIADFARPPIWYRGYRLWLGPNGDGLWA
                     VAAAANRDHAPAFCLSAGRMHPQVFPFGFPDEIGMCQADAAIHRIMGGEA"
     gene            complement(3792..4559)
                     /locus_tag="VKQ27_19025"
     CDS             complement(3792..4559)
                     /locus_tag="VKQ27_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91084.1"
                     /translation="MLGGALDPDEEQARTLALAWHLSAEPVVATALHENHREMLAMLQ
                     RAVRNRLVSRDDGDQVTLDVVSFEPALQAAYRLACDDDEVRRIVESAHEARKREEAGR
                     QAAEKQRERVEAERLALLISDRAAMLYDLGVAWKEHRNPRRRTLAPLVIALAGALHDN
                     TLYFRPSRADSASPRPLYAFAANEDVAWIVRQLLKDPVGRAALKVLSRIDLDLPRPDR
                     RWRPVEPIELWLISVGRRGKGTGRGPDRGEGRGGRGD"
     gene            4956..5729
                     /locus_tag="VKQ27_19030"
     CDS             4956..5729
                     /locus_tag="VKQ27_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91085.1"
                     /translation="MSTLLHEMRMEPIDQPLVPDIAGVELTDCRQLFVGFHPVGCSAD
                     CLDTMDLDRPLHATDSRLLLDADFVKIGTGSTDNWRAADQRLFVRLQASDDVCRELPK
                     PPQDRQAVGPDATPKLFPPLFERRCRWPRLDPCGTSCKGLDPLKLREDPVLEFGSLVS
                     QRRHLDLRTRKVIRPGFERSIDLDEMAPCRGAPRLNRSPVRDLRTTGFTCVDHRHDAR
                     CGGCHHLSPRPGDPVEKADLLEGPFIIAEIVDDALIALD"
     gene            5937..>6215
                     /locus_tag="VKQ27_19035"
     CDS             5937..>6215
                     /locus_tag="VKQ27_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91086.1"
                     /translation="MILRPTRDAHLSIAVWIETPGGPDPFELAIDRDGHPPPQCRQEE
                     RVAGLAAITLAPRLVRDRDSAPTPLVCGQFDIPAALGLDPAAWPRIDQQ"
BASE COUNT         1164 a         2014 c         1916 g         1121 t
ORIGIN      
        1 cgtcgttggc gataccctca acatgatcgc gtacaacatt tcaaaaagcg agatcgagtg
       61 ggtgggcgtg cgccacgaag aggctggtgc cttcgccgcg cagggcgaag cctcgttaac
      121 cggcaacctg accgcagtcg ccggaagctg cggtcccggc agcttgcatt tcatcaacgg
      181 catcgtcgag gcgaaccgca atcgcgcacc cgtcatcctg atcgccagcc agatcgttcg
      241 cgacgaattg gggttcgact ttccccagga gctcgacttc aaggcgatat acgggagttg
      301 taccgtctat tgcgacatga tcctgacgcc cgaacaggcc cgacgcaaaa ccgtcatggc
      361 ctgtcaggca gcgatcgcga agcgcggagc ggcggtcctg atcgtgccgg tcgacatctc
      421 gcattccgac gtccaggatg acgtaccgtt cgccgtgcag acgagccatc cggtggtccg
      481 tccgaccgat gacgatctcg acgagatcgc cggcatcatc aattcgggcg agcagatcgc
      541 catatatggc gggtcaggtt gtcgcggcgc gcatgacgag attcttcgtg tagccgaaat
      601 cctcaaggcg ccgattgccc acacctcccg gggcaaggac tcgctcgaat atgacaatcc
      661 gtacaatgtc gggatgaccg gaatcatcgg caacgaggca gcctatcgcg ctgttcggga
      721 atgcgacacc ttgctgctgc tcggcgcgga ttttgcctgg cggcagttct accctaaaca
      781 tgcgaagatc gtgcagatcg acatcgaccc ggcccatctg ggccgccgcc atcctgtgac
      841 ccgagggttg gttggcgaca tcaaggcgac cctcgaggcc ttgatccctc gactgcatga
      901 gaaaatggac acagatttcc gcgatcgctt tgtcaagcgc caccgagacg tgatcgccag
      961 tcagaaagag aaagccgcgc ccaagcggcg cgacacgatt tccgggatcc atcttaccga
     1021 gcttatcgat cggatggcgg ctgataatgc gcttgtcgca ggagacgacg gaacgccgac
     1081 cgtctggatg catcggtatt tcacagccac gcgcgaacgg cgcttttacg ccagcctcct
     1141 gcatggaacc atggcggcgg ccatgccgac cgcactcggc ctgcaaaagg ctgcgcccgg
     1201 ccgccaagtc atcgctctct caggcgatgg cggaattggg atgctattcg gcgatctgat
     1261 gaccgcggtc caagagaagt tgcctatcaa ggtagcggtg ttcgacaacg gaaagctggg
     1321 tttcatcgat atcgagcaga aatcggaagg aatgctgccg ctttacaccc atctgcaaaa
     1381 tcctgatttc ggcgccgtcg cagaggcaat gggactatgg ggccgcaccg tcgccaaggc
     1441 aggcgagatt gaggccgcga ttggagagtg gctcgctcat ccgggccctg ccctgctcaa
     1501 cgtgaaggtt gaaagcacga ccttggtgat gccgccagcg atcgaatttg gaccagctta
     1561 tggaatggcc ctttattcgg cgcgtgcggt gctcggcgga caaacccaag agctgatgga
     1621 aatgatccag gaaaacttcc tgtgaccaga cgatcattgc ctgtgcagca gcgagggtta
     1681 ggcgaacatg ctcgatggcg ctgtgatcga tccggcgatc ccagtccagc gggcctagaa
     1741 aggcgtgttc ggcacagggt tagggtacca ctatccggtt gaggaacgag cgaggtccgc
     1801 cagcgcatgt tggcggacct cgccgtgacg cggatcgaac cgaaggcatg tgcccgcccc
     1861 agcgcggcta ctcggatctg cgccgtgagg ccagcgccct cgatatcgcg atttccttca
     1921 tctaagcgga ctccggtccc cacccgaaac caacgtcgac tgttgggaac agcaaaaccg
     1981 ccgctgaacg atcggcattt cggctggtgt gacgcctgtc acaccaattg cgcttcgaga
     2041 tatcggcgga ccgcctgtag gtccgcgatc gttcctcccg ccatacgggc ggcgggggtc
     2101 atcccggcaa aggtcggggc gttattggtg gcccgcatcc aattatctgc gcgttcgggg
     2161 atcgggagca gcgtgttgat cgccttgtag atcccaagtg cgtaactcac gcgctcgagc
     2221 gtccccgccg cgagctgtgg tgcttcgttt gacctcaggg catccagctc tggtttggct
     2281 tccagaccta gaattttgag ttgctcactc tccgtgagat tccagcggtc ggcgatccgg
     2341 aaaaaaaacc gcaacccagc agcttctgcc tcatccattt gatcgttttc ctcataactg
     2401 atgaaccaca atcgatcatg acatttgagt tagcgacagc gcgcggctct gagatcgagc
     2461 agttccttat gcagtagtcc ggggcccttc ttgttcagca acggagtcgg cgcagcgaaa
     2521 ccggtcggta gatgtttcag cggcagactg agaatatgcc catgcatcgc catctcgtgg
     2581 cgactgtctc caggcacatg gagcacgaca aggctggaca acgcccatcg gcacgccaac
     2641 cgatcagcat aggctatcac ttcggccatg cagtcgtgcc gggtgatcgc cttcggataa
     2701 tagactgtcg tcaccagccc gaccacatcc tcctccggca gatggtgtcg atcgaaagcc
     2761 gggaagagca attcgggggg ctggaaggcg tctggcacac gcggcgctag gaccaccttc
     2821 caatcagccg ctgtgatcgg ccacaggtgc ggattgatcc ggtctccatc ggtgggacat
     2881 cgtggctgga gcttggcttg cgccaggctg ctcgcggcgg ccccgagcga agcgcgcgcc
     2941 acgccggtcc gaaaactctc gaccccgggc ggcgtccgat cctcggtcat gcgcaaggcg
     3001 gcggtgggac tgatccacat ggctcaggcc tccccaccca tgatacgatg aatggccgca
     3061 tcggcctgac acatgccgat ctcatccggg aaaccgaagg ggaatacctg gggatgcatc
     3121 cggcccgccg acagacagaa tgccggagcg tgatcgcgat tagccgcagc ggcaaccgcc
     3181 caaagaccgt cgccgttcgg accaagccag aggcgatagc cgcgatacca gatcggcggc
     3241 cgagcgaaat cagcgatagc gatcccaacc cggaacgcga acgccccgat tccaatcgga
     3301 tgcacgatca gcgagggggc accgatgcgc ataacctccg cctcgacctc accgaccgca
     3361 cgatcggtga ccacggtcca tggcacgatg agcgcatcaa ggtctggcgc gaacaggtag
     3421 ctgcgggcag agctgcgcgc ggcctcgggg cagccgtgga acggttgacc ggcgagcatc
     3481 atgatagcct cggcggcgat ccgagctcga acattttcat gcccgtgatg ggcgggcgat
     3541 tttccaatca tatctgtctt tcggttgcgg cgggtggctg caaccacgcc ctgtttcaca
     3601 ttccttggca gccactggct gcgtcctcac ccctccctgt cacggcgcga cattctatcg
     3661 agggagccgt atgctccctg gcatctagat gcatggcggg cccgttgtgg gggcgatcaa
     3721 cggcatcatg gaacggccga cggtgccatg attgacgaag gagaagccgt catcctcccc
     3781 cggcggcgaa gtcagtcgcc gcgtccacca cgcccctcgc cgcggtcagg gccccgtccc
     3841 gtccccttac ccctcctgcc gacactgatc agccacaact cgattggctc cacgggccgc
     3901 cagcgccgat ccggccgagg aagatcgagg tcgatccggc tgagtacctt gagcgccgcg
     3961 cggccgaccg ggtccttcag cagctgcctg actatccagg cgacgtcctc gttggccgcg
     4021 aaggcataga gagggcgcgg ggaggcagag tccgcccggg atgggcggaa gtagagggtg
     4081 ttgtcatgca gggcgccggc aagcgcgatg accagcgggg cgagggttcg ccgtctcgga
     4141 ttgcgatgct ccttccaggc cacaccgagg tcgtaaagca tcgccgcgcg atcgctgatc
     4201 aagagcgcga gcctttccgc ctccactctc tcccgctgct tctccgcggc ctgccggccg
     4261 gcctcttcgc gcttgcgcgc ctcatgcgcg gactccacaa tccgccggac ttcatcgtcg
     4321 tcgcacgcta accgataggc ggcctgcaac gccggctcga aggataccac atcgagagtg
     4381 acttggtcgc cgtcgtccct ggagaccagc cgattgcgca ccgcgcgctg taacattgcg
     4441 agcatctccc ggtggttctc atgcaacgca gtcgcgacca cgggctcggc cgaaagatgc
     4501 cacgcaagcg cgagtgtccg cgcctgttcc tcatcaggat caagagcacc gcctagcaag
     4561 cgcacagcat cctgcggtgg aggctcggga atccatgcca ccccctggcg cacaagcggc
     4621 aagcaaggcg tcgatcggat cctgcggaga gtttcggcaa cgagttgagc gcgaattctc
     4681 cgctccgttg gcgacggcaa cggccaagac cagccaagcg caagatcacc atccgtaccc
     4741 gcggcggagt gctgatcatc ggtgacccga cccgccagcg tcccgtcatt cgccggagtt
     4801 cggaccggtt cggcgtctgg cttcatcttt agcggatcca tcgccgggga agcagtggaa
     4861 acggtctcgg atcgcacccc cggtgcagga ggagccgttt gatgatgcgg gcttggcagc
     4921 tcgtatccgg cgcgatcatt tgccgccgcg ttgtcatgtc gacgctgctc cacgagatgc
     4981 gcatggagcc aatcgatcag cctctcgtcc cggatatagc gggcgtagag ttgaccgact
     5041 gtcgccagct gttcgttggt ttccaccccg tcggctgctc ggccgattgt ctcgacacca
     5101 tggacctcga tcgcccgctt cacgcgaccg atagccgcct cctgctcgac gcagattttg
     5161 tgaagatagg gaccggctct accgacaact ggcgtgctgc ggatcagcgt ctgttcgtcc
     5221 ggctccaggc ctcggacgat gtatgtcggg agctgcccaa gccccctcaa gaccgccagg
     5281 cggtcggccc ggatgcgacg ccgaagctgt tcccaccgct cttcgagcga agatgtcggt
     5341 ggccccggct tgatccgtgc ggcacttctt gcaagggcct cgatccgctc aagctccggg
     5401 aggatcctgt gctcgagttc ggcagcctcg tgagccaacg ccgccacctg gacctgcgca
     5461 cgcgcaaggt catccgccca gggttcgagc gctcgatcga tctcgacgag atggcgccgt
     5521 gccgaggcgc gccgcgcctc aatcgcagtc cggttcgcga cctccgaacg accggcttta
     5581 cctgcgtcga tcatcgccac gacgcgcgct gcggaggttg ccaccacctc agcccgagac
     5641 cgggcgatcc ggtcgagaag gccgacctcc tcgagggtcc gttcatcatc gccgagatcg
     5701 tggacgacgc cctgatagcg ctggactagc tcacgaaacc gagcctgccc gggggcggtg
     5761 gcgagcatgg tcagtgccgc ttcgattcgt gccagcagcg cggtccgccc ggcctgtcgt
     5821 tgggcatcgg cagagcgcat ttcggcgaca cgatcacacc aggcggtccg ctggttttgc
     5881 cggccgacat gggttgccac ccccgctcgc tcgagcgccg aggcccgcgt tccgaggtga
     5941 tcctgcggcc gacgcgcgac gcccatctca gcatagcggt gtggatcgag acgccgggcg
     6001 gccccgaccc gttcgagctc gccattgatc gcgatggcca ccctcctccg cagtgccgtc
     6061 aggaagagcg cgtcgccggt ttggccgcca tcacgctggc gccgcggctt gttcgcgatc
     6121 gcgattccgc gccgacgccg ctcgtctgcg ggcagttcga catcccagca gcccttggcc
     6181 tcgatccagc ggcatggccg cggatagacc agcag
//
LOCUS       DATGEM010000617         9120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_428068, whole genome shotgun sequence.
ACCESSION   DATGEM010000617 DATGEM010000000
VERSION     DATGEM010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428068"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VKQ27_19040"
     CDS             complement(<1..431)
                     /locus_tag="VKQ27_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY91087.1"
                     /translation="MPDEASVVPAQLLVEHVTRFTDDDLHALCEATAAAIIDGGGFGW
                     VNPPGRMALETYFRGVLLVPERELFVGRLNGVVVGSAHLVRPPRNNEAQAHAAQLMHS
                     YIAPYARGHGLARMLTVHVEDRARDLGYYVLNLDVRETQEAA"
     gene            complement(435..1070)
                     /gene="hisH"
                     /locus_tag="VKQ27_19045"
     CDS             complement(435..1070)
                     /gene="hisH"
                     /locus_tag="VKQ27_19045"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLY91088.1"
                     /translation="MKVAVVDYGSGNLTSASRALALAAEREGLAAEVKVTADPDYVAS
                     ADRIVLPGQGAFADCSHGLAAVRGMREAIEVATEAGRPFLGICVGMQLMAERGLEHEV
                     VEGFHWIDGEIAEMDAAGLRLPQMGWNGLDFIPGRHRLLDGLTPGDHVYFVHSYALRG
                     GDPREAIAMTDYGGPIVAMVASGNRAGTQFHVEKSQEVGIRILGNFLRWAP"
     gene            complement(1165..1542)
                     /locus_tag="VKQ27_19050"
     CDS             complement(1165..1542)
                     /locus_tag="VKQ27_19050"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HLY91089.1"
                     /translation="MKFWSAHVRAGAAPVLVREGFSWGALFFGPIWLAVHRAWIGAAV
                     SLAAFVIIGFTTHDGVQATLLVTLVVLLGLSGHDLRRWSLDQRGFLLAQVVTGRSELD
                     ALSHLLERRPDLKGSFMPPESAR"
     gene            complement(1539..2129)
                     /gene="hisB"
                     /locus_tag="VKQ27_19055"
     CDS             complement(1539..2129)
                     /gene="hisB"
                     /locus_tag="VKQ27_19055"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HLY91090.1"
                     /translation="MARTANLTRTTSETDITLTLSLDGSGQSEIATGIGFLDHMLTAL
                     ARHGLFDLTVKAKGDLHIDFHHTTEDVGIVLGQAFAKALGDKRGIRRFGHAVVPMDET
                     LAEAAVDISGRPFLVWDVVFERPKIGEMDTELFEEFFRAFAINALVTLHIIRRSGRNA
                     HHVAEACFKATARALRVASELDPRVGDAIPSTKGAL"
     gene            2306..2659
                     /locus_tag="VKQ27_19060"
     CDS             2306..2659
                     /locus_tag="VKQ27_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91091.1"
                     /translation="MICAINDAFNRLNIQSTAAQLGTLASTGDFLDICEDLGLLAPLH
                     RGNFAAYKTNLPIPPVIQSMLTAAFRHALTATPQPIPLQILIVSGTQDIVTITSTATE
                     ISVLLTRNDPPVSGP"
     gene            2685..5750
                     /locus_tag="VKQ27_19065"
     CDS             2685..5750
                     /locus_tag="VKQ27_19065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY91092.1"
                     /translation="MTVRNKETASPSVPAAAEVDGEQRRVVLMFADVSGSTELIEQLD
                     PEQAARLLDPLIGVMAETVERYGGTVSPRGDGVLATFGIGGTAEDSAARACFAAFEIM
                     QGLRDASAQVRIGIHQGEVALLSGPRRQSRENLFGPAIHIAARLEQSTEPGTASISTD
                     AYGLVREFVDAEPLPPVEVRGLSRPLQRMRLLRVRSTSRWRARLAQGLAPLVDRQAEL
                     AAITRFLREATRPGVHYLQVLGPPGTGKSRLVHEVLRSDPGRACYLVTLTGPLHRGYA
                     GYDPIAHWLRELAGATDADAAADLVKSLPGIDALAEAERERLDRYLDVGTAANARLAD
                     IESLMPLRVIKPIATIIAKAAGDRSVLISCEDTETLGDDRLAQITALADAVTNGGLPV
                     ILIVSSRRAIKPPGSNIATRKTLRLQSLGQDDARRLLARLRPELHRQPKLVEAVLEKA
                     AGNPLFLEEVAARMGTGAEQSEAIPDRIDALIGDRLARLPRQVQTLLLICSVLGSVFS
                     PGALSALAQQCEQSVQQQLAKLKAEHLLYEVPGSSARLSFGHSLIRDVAYNTLLPSRR
                     RSLHERALRLLEQQPQTANLEDLCHHAVQARLWAEALRYLQQAAVAAAERAAYASAEQ
                     HLRRALKIAEQQPQDRPMQRTMVEIMMGLRAVVALDLRQVEADELLDRAEKLGADLEP
                     AIRLAIQLRRVRALNTAGRLREAIGVASTTRQQGRESGHIAVQCTAAYMLGQSYFYVG
                     RFAAGDTTLTEAANLAQAGPHSMQFAVGDSPAMIQATRAGVRGFLGRFGAAEADAAQA
                     ITIAQSQQAIYDLAFAHFASGLVQLRQRHLIEAETAFRAGLDITERHSLRALQPSLNV
                     GLGHTLLLSGSTNAAIAALVDAHEAATQGNRVLNQMWAAVGLAAAHGSTGGTLSALRY
                     ADEAVALGARHTLRGLLVSALRCRGALLATEEENHAAGVRNVRQALALARKLGMRPDA
                     AHCLTALAAILDDNHARNEALAIYQALGMQPWAEHVLGQPPVPLSIIAA"
     gene            complement(5855..7282)
                     /locus_tag="VKQ27_19070"
     CDS             complement(5855..7282)
                     /locus_tag="VKQ27_19070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91093.1"
                     /translation="MSASTSATTLSRSGRWLAFAVLSIGALLPSIDFFIVNVSLPSIH
                     ASIGASPAELQLVVSGYAGTFAVFLITGGRLGDLYGRRLIFMLGMAGFVATNMLCGVA
                     ATPVQLVIGRILQGVSAAMLGPQVLGSIRALFPSESELAKALSVYGMLVGVAAAIGQF
                     SGGALVEWSPFGWGWRTIFLVKLPIGLVVLVAAWLVVPETSGSQRQRLDIGGAALVSL
                     ALACLVLPLSEGRQHGWPAWTFVLLALMPVFGAMFVWLEKRLTYGGGGPLVDLRLLAI
                     PSFRRGVLVGTLFFFTTAFYVLFAIYEQEGYGTDPLYTGLAILPYGIGLFVGPLVTGH
                     LVRLRPWLLTLGMLIQVTGYALVAVVIVLGFDGWRVTLAVLLAGFGQGIAYPRLFNTA
                     LGDVPPAQAGVAAGVLTSALQIGAALSVAAIGSLFFTVLGAGVGRDAYAHAFGVAQAA
                     TSFALAIAMVLSIPRRPSSRGGMRR"
     gene            7372..8451
                     /locus_tag="VKQ27_19075"
     CDS             7372..8451
                     /locus_tag="VKQ27_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HLY91094.1"
                     /translation="MTSDPAGLYARLGVDPSAPPDAIVTAFRRKARVLHPDVPRTGNA
                     EAFVWVKEAYDVLSDPPKRAAYDRSARAAAAAPPTAPAIEEVPLLWPKFSDLPLALWI
                     GFGAVFCLASGMAVYEFTRPTPPRPLAQIRPAAPSVTSLSTAPANVASAGSATHYVLP
                     GGGAAMVWQRDATRDAFVPAGRVADFTSVQVVGVLAHSGLVGIRLADGGIGFLDPDRL
                     APGDQTTAHRAYCGYNAGAPPNNGEVLSRRGTGSGRLTIENRGSEPAVVKLRDTDSQA
                     VVSVYVAAGHAATVTDLPTQSYRPEFALGELWSRACDGFTAGMRAQRFIGYGPLEGLS
                     PLIVPPSLSAAPEPVDIPDAAFEQD"
     gene            8454..>9120
                     /locus_tag="VKQ27_19080"
     CDS             8454..>9120
                     /locus_tag="VKQ27_19080"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY91095.1"
                     /translation="MSIRLQESLRGLFYAPFYVALAHDAYAAEGVDVEFVSSPRPGDA
                     AVNVMNGTVDVCWGGPMRVMQAYQEIPGCDLVCFAEVVTRDPFLLIAREPRPGFSLRD
                     LLGLRVATVSEVPTPWMCLQEDLRRAGIDPAALSRVADRTMAENVAALTRGDVDVVQV
                     FEPFASSGGFPVWYAQADRGATSYTTFYARRGLLAARHDELLRMVRAIHRTLGWVAEA
                     DGNA"
BASE COUNT         1694 a         3132 c         2855 g         1439 t
ORIGIN      
        1 gccgcctcct gggtctcgcg cacatccagg ttcagcacgt aatagccaag gtcccgcgcc
       61 cggtcttcca catgaaccgt cagcattcgc gccaggccat gcccgcgcgc atacggagcg
      121 atgtacgaat gcatcaactg cgcggcatgc gcctgcgcct cgttgttgcg cggaggacgc
      181 accagatgtg ccgacccgac gacaacacca ttcaggcggc caacgaacag ctcccgctcc
      241 ggtaccagca acacgccgcg gaagtaagtc tccagcgcca tccgccccgg tggattcacc
      301 cagccgaacc cgcctccatc gatgatcgcc gcggcggtcg cttcgcacaa agcgtgcagg
      361 tcatcgtcgg taaatctggt cacgtgctcg accagaagct gtgccggtac tacagacgct
      421 tcgtcaggca tgtctcaggg cgcccagcgc aggaaattac cgagaatacg aatgcccact
      481 tcctggctct tctcgacgtg gaactgcgtc ccggcgcgat tgccggatgc caccatcgcc
      541 acaatgggtc ccccatagtc ggtcatcgca atcgcctcgc gcgggtcgcc ccccctgagg
      601 gcgtagctgt gcacgaagta gacgtggtca cccggtgtca gcccatcaag gagcctatga
      661 cgccccggaa tgaaatccag cccgttccac cccatctggg gcagccgcag ccctgccgcg
      721 tccatctcgg caatctcgcc gtcaatccaa tggaagccct cgaccacttc atgctccagc
      781 ccgcgctcgg ccatgagttg catgccaacg cagatgccca ggaacggccg tccggcctcg
      841 gtcgcaacct cgatcgcctc gcgcatgcct cgcacggcag ccaatccatg cgagcagtcg
      901 gcaaacgccc cctgccccgg cagcacaatg cgatccgccg aggcaacata gtcggggtcg
      961 gcggtcactt tcacctccgc cgccagccct tcccgttccg ccgccagcgc cagcgcgcgc
     1021 gaggccgacg tcaggttgcc gctgccatag tccaccaccg ccaccttcat gcgtggaaac
     1081 gggcgttagc cccgccttca tagcccgccg ccgggcttgt cccgatggtc ttgggcgtcg
     1141 tcgtgggcgg gcttgaccgg cccatcatcg cgcgctctcc ggcggcatga agctgccctt
     1201 caggtcagga cgccgctcca gcagatgcga cagcgcgtcg agctcgctgc ggccggtgac
     1261 cacctgcgcc aacaggaagc cccgctgatc cagcgaccag cgccgcaggt cgtgtcccga
     1321 caagccaagc aacacaacca gcgtcaccag aagcgtagcc tgcacgccgt catgggtcgt
     1381 gaacccgatg atgacgaacg cggccaggct gacggcggca ccaatccatg cccgatgcac
     1441 cgccagccag atcgggccaa agaacagcgc tccccagctg aagccctcgc ggaccagcac
     1501 cggagcggcg ccggcgcgta catgcgcact ccagaacttc acagcgcgcc cttggtcgag
     1561 gggatcgcgt cccctacccg cggatcgagc tcagacgcca cgcgcagggc gcgggcagtg
     1621 gccttgaagc atgcctccgc gacatggtgg gcgttccgtc ccgagcggcg gatgatgtgc
     1681 agcgtcacca gtgcatttat ggcgaaggcg cggaagaact cctcgaacag ctcggtgtcc
     1741 atttcgccga tcttggggcg ctcgaacacc acgtcccaga ccaggaatgg ccggccggaa
     1801 atgtccacgg cagcctccgc cagcgtctcg tccatcggga caacagcgtg gccgaaccgg
     1861 cggatgccgc gcttgtcgcc cagcgccttg gcaaaggcct ggccgagtac gatgccgaca
     1921 tcctcggtcg tgtggtggaa gtcgatgtgc aggtcaccct tggccttgac cgtcaggtcg
     1981 aacaggccgt ggcgcgccag cgcggtcagc atgtggtcca ggaagccgat gccggtcgcg
     2041 atctcggact gccccgaccc gtcgagcgac agggtcagag tgatgtccgt ttccgaggtt
     2101 gtccgcgtca ggtttgccgt gcgggccatg gacggggact tagcgtgagc acgcacagcg
     2161 gtccagccag ggcaacaaca atcacgtgtt tcggtactgg tttactgacc cagccgaagc
     2221 ggccttaatg tgctctgcag aacgaggggg tggaaagatg ggaagcgaag ccgtaatcag
     2281 aggcggaccg agtagccgtg tccaattgat ctgcgccatc aacgacgcgt tcaatcgttt
     2341 gaacatccag agcaccgcgg cgcaattggg gacgctcgcc tcgactggcg acttcctgga
     2401 tatctgcgaa gatctgggac tcctggcgcc tctgcaccga ggcaattttg ccgcctataa
     2461 gaccaatctc cccatcccgc cggtcatcca gtcgatgctg accgcggcgt tccgccatgc
     2521 gctgaccgcg acgccgcagc cgatcccact gcagatcctg atcgtctccg gcacgcagga
     2581 catcgtgacg atcaccagca ccgcaaccga gatatcggtc ttgttgacac gcaacgaccc
     2641 gccagtatcg gggccatgag aatcaacggc acgtaggcat tcacatgacg gttcggaaca
     2701 aggagacggc atctccttcg gttccggccg cggccgaggt cgatggcgag caacggcggg
     2761 tcgtgctgat gttcgccgat gtgtcgggct ctaccgagct gatcgagcaa ctggatcctg
     2821 aacaggccgc aaggctgctc gacccgctga tcggcgtgat ggcagaaacc gtcgagcgct
     2881 atggcggcac ggtcagcccg cgcggcgacg gcgtcctcgc gacattcggc atcggcggca
     2941 cggccgaaga cagtgccgcg cgcgcctgct ttgcagcttt cgagatcatg cagggcctgc
     3001 gtgacgccag cgcgcaggta cgcattggca ttcaccaggg tgaggtcgcg ctgctgtcgg
     3061 gcccgcgccg gcaatcgcgg gagaacctgt tcggtccggc gatccacatt gccgcccggc
     3121 ttgaacagtc caccgagcct ggcacggcct cgatcagtac cgacgcctat ggactggtgc
     3181 gcgaattcgt cgatgccgag ccgctgccgc cggtagaggt gcgaggtctg agccggccgc
     3241 tgcaacgaat gaggctcctc cgtgttcgtt caaccagtcg ctggcgtgcc aggctggcac
     3301 agggtttggc gccgctggtg gatcgccagg ccgagttggc ggcaataact cggttcttgc
     3361 gagaggccac ccgtcccggc gttcactacc tccaagtgct tggaccgccg ggaaccggta
     3421 agtcccgact ggtgcacgaa gtgctgcgca gcgatccagg tcgtgcctgc tacctcgtca
     3481 ccctgactgg accgctgcat cgcggctacg ccggatatga tccgattgcc cactggctgc
     3541 gcgagctcgc aggtgcgacg gacgccgacg ccgcggcaga cttggtgaaa agcctgcccg
     3601 gcatcgacgc gcttgccgag gccgagcgcg aacgactgga ccgctacctc gacgtgggca
     3661 cagcggcgaa cgcacggctc gctgacatcg agtcgctgat gcccctgcgg gtgatcaagc
     3721 ctatcgcgac cataatcgcg aaggccgcag gggaccgttc cgtcctgatc tcctgtgagg
     3781 ataccgagac acttggcgac gatcggctgg ctcagatcac cgcactggcc gatgcagtga
     3841 ccaatggcgg actgcccgtc atcctgattg tgagcagccg gcgtgcaatc aagccgccgg
     3901 ggagcaatat cgccacccgc aagacactgc ggcttcaatc attgggacag gacgatgcgc
     3961 gacggctcct ggcgagattg cgtccggagc tgcatcgaca gccgaagctc gtcgaagccg
     4021 tgctggaaaa ggccgcaggg aacccattgt tcctggagga ggtcgccgcc aggatgggaa
     4081 ccggcgccga gcaaagcgag gccattcccg accggatcga tgcactgatc ggcgatcgcc
     4141 tcgccaggct gccgcgtcag gtccagacac tgctgttgat ctgctctgtc ctcggcagcg
     4201 tgttctcgcc aggcgcgctt tcagccctgg cacagcagtg cgaacagtcg gtccagcaac
     4261 agctcgccaa gctcaaggca gaacatctgc tgtacgaggt acccgggagt tcggcgcgcc
     4321 tgagcttcgg tcactctctc attcgcgacg tggcgtacaa cacgcttctg ccttcgcggc
     4381 gtcgcagttt gcacgagcga gcgttgcggc tgctggagca gcagccgcag acagcaaacc
     4441 tcgaggacct ttgccatcac gccgtgcagg cgcggctgtg ggcagaagcg ctgcgctacc
     4501 tgcaacaagc ggctgtcgcc gctgccgagc gggccgccta cgcctccgcc gagcaacatt
     4561 tgcggcgtgc gttgaagatc gctgagcaac agccgcaaga ccgaccgatg caacgcacca
     4621 tggtcgagat catgatggga ttgcgcgcgg tggtcgcact cgacctgcgc caggttgaag
     4681 ctgatgagct gcttgatcgt gcagagaagc tcggagcaga cctcgaacca gcgatccgac
     4741 tcgcgatcca gctacgacgt gtgcgcgccc tcaacaccgc gggtcgactg cgcgaagcaa
     4801 taggcgttgc cagcacgacc cgacaacagg gacgtgagtc gggccacatc gccgtgcagt
     4861 gcaccgcagc gtatatgctg ggacaaagct acttctacgt tggccggttt gctgccgggg
     4921 atacgaccct taccgaggcc gcgaacctcg cgcaggccgg tccccactcg atgcagttcg
     4981 ccgttggcga ttcgccggca atgatacagg caacgcgcgc tggcgtccgt ggcttcctcg
     5041 gccgctttgg cgctgcggaa gccgatgctg cacaagcgat cacaatcgcg cagtcgcagc
     5101 aggcaattta cgatttggcc tttgcccatt tcgcatccgg cctcgttcag cttcgccagc
     5161 gccatctcat cgaggcggaa acggcgttcc gcgccggatt ggacattacg gaacgacata
     5221 gtctgcgcgc gttgcaacca tcgttgaacg ttggcctcgg acacacgctg ttgctgagcg
     5281 ggtccaccaa tgcggcgatt gccgctctcg tcgacgcgca cgaagcggcg acgcagggca
     5341 atcgcgtatt gaaccagatg tgggcagcgg tcggtcttgc cgccgctcac ggcagcaccg
     5401 gcggtacgct gtcagcgctg cgctacgccg atgaggcagt ggcgctcggc gccaggcata
     5461 cgctgcgcgg gctattggtc agcgcactgc gctgccgcgg tgcgctgctc gcgacagagg
     5521 aagagaatca cgccgctgga gtccgcaatg tccgccaggc cctggccctg gcgcgcaaac
     5581 tcgggatgcg gcctgatgcc gcgcattgcc tgaccgctct tgccgctatc ctggacgaca
     5641 accacgcacg caacgaggcg cttgccatat atcaggcgct tggcatgcag ccatgggcag
     5701 agcacgtgct gggacagccg cccgtgccgc tcagcatcat cgcggcctga ctaccgccac
     5761 accaggagcc aacacaaagt tcgcgaagag acgcgcttcg cccccaggtc ccgaccccca
     5821 cgtaagaaag cgcggccccc atggaatgcg ctggtcagcg gcgcatccca ccacgggatg
     5881 agggccggcg cggtatcgac agcaccatcg cgatcgcgag cgcgaagctg gtcgccgcct
     5941 gcgcgacccc gaacgcatga gcataggcat cccgccccac ccctgccccc aacaccgtga
     6001 agaacaggct gccgatagcc gcaacgctca gcgccgcccc gatctgcagc gccgaggtca
     6061 gcacgcccgc agcgacccca gcctgcgccg gcggcacatc ccccaaagcc gtgttgaaca
     6121 gccttggata agcgatgccc tgcccaaatc cagccagcag cacggccaag gtgactcgcc
     6181 agccatcgaa gcccagcacg atcacgaccg ccaccaaagc gtagccggtc acctggatca
     6241 gcatgccaag cgtcagcagc cacggccgca accgcacaag gtggccggtc accagcggcc
     6301 cgacaaacaa gccaatgccg tacggcagga tggcaagccc ggtgtacagc gggtcggtgc
     6361 cgtagccttc ctgctcgtaa atcgcgaaca gcacataaaa agcagtggtg aagaagaaca
     6421 gtgtgcccac cagcacgcca cggcggaaac tggggatcgc cagcagccgc agatcaacca
     6481 atggaccgcc gccaccatag gtcagccgct tttccagcca gacgaacatc gccccaaaca
     6541 ccggcatcaa cgcgagcagc acgaatgtcc aggcgggcca gccgtgctgc cgtccctcgg
     6601 acagcggcaa cacaagacag gccagcgcca acgaaaccag ggccgcgcca ccgatgtcca
     6661 gcctctggcg ctggctgcca cttgtctccg gcaccaccag ccaggcggcg accagcacca
     6721 ccagacctat cggcagcttg accaggaata tcgtgcgcca tccccagccg aacggactcc
     6781 attcgaccag tgcaccaccg gagaactgcc cgatcgcggc agcaaccccg accagcatgc
     6841 cgtacacgct gagcgccttc gccagttcgg actccgatgg gaacagcgcc cggatcgatc
     6901 ccagtacctg tggtcccagc atcgccgccg atacgccttg caggatacgg ccaatcacca
     6961 gctgcacggg cgttgccgct accccacaca gcatattcgt cgcgacgaag ccggccatgc
     7021 cgagcatgaa gatcagccgg cgcccataca gatcacccag ccgcccacct gtgatcagga
     7081 acaccgcgaa ggtaccggca tagcctgaga ccacgagttg cagttccgcc ggcgaggcgc
     7141 cgatggaggc atggatcgac ggcaggctga cattcacgat gaagaagtcg atcgaaggca
     7201 gcagcgcgcc aatggacagg acggcgaagg cgagccatcg gcccgagcgg gataacgtgg
     7261 tggccgacgt tgaagctgac acgggacgga acgtttcctg gacgacttgg cgcctgagta
     7321 gcggcgggtc ccggctgcca caagggcagg ggcagcgcct attatggcgc gatgaccagc
     7381 gaccccgccg ggctctatgc acgattaggc gtcgatccgt cggcaccgcc cgatgcaatc
     7441 gtgacggcgt tccggcgcaa ggcgcgcgtg ctgcacccgg acgtgccacg gacgggcaac
     7501 gccgaggcat tcgtgtgggt caaggaagcc tacgacgtgc tcagcgatcc cccgaagcgc
     7561 gcggcctacg accgctcggc gcgtgcggcc gccgcggcgc cccccactgc ccccgcgatc
     7621 gaagaagtgc cgctgctctg gccgaaattt tccgacctgc cgctcgcgct ctggatcggc
     7681 ttcggcgctg tgttctgcct tgcctcaggg atggcggtgt atgaattcac ccggcccaca
     7741 ccgcccaggc ccttggccca gatccgtccc gccgcaccgt ccgtcacctc gctgtcgacg
     7801 gcacctgcca acgtggcgag cgccggcagc gccacacact acgtgctgcc gggtggcgga
     7861 gcggccatgg tctggcagcg tgacgccacc cgtgacgcat tcgtgccggc cggtcgcgtc
     7921 gccgacttta cctccgtgca ggttgtgggc gtgctcgcgc acagcggcct cgtgggcatc
     7981 cgtctggcag acggcggcat cggcttcctc gatcccgatc ggctggcacc cggcgaccag
     8041 accaccgcac accgcgccta ttgcggctac aacgccggcg cgccgccgaa caacggcgaa
     8101 gtgctgagcc ggcgcggcac cggttcggga cggctcacaa tagagaaccg cggcagcgag
     8161 ccggccgtgg tgaagctgcg cgacacggac agtcaggcgg tggtcagcgt ctatgtcgca
     8221 gccggccacg cggccaccgt gacggacctg cccacgcaat cctaccgacc cgaatttgca
     8281 ctcggcgagc tctggagccg cgcctgcgat ggcttcaccg ccggcatgcg ggcgcagcgc
     8341 ttcatcggct acggccccct ggaagggcta tcgccattga tcgtgccacc gagcctttcc
     8401 gccgctcccg agccggtgga tattcccgat gcggcgttcg aacaggacta gccatgtcga
     8461 tcaggttgca ggagtcgttg cgcgggctgt tctacgcgcc gttctatgtg gcgctggcac
     8521 acgatgccta tgccgccgaa ggcgtggacg ttgagttcgt cagctcgccg cgtcccggcg
     8581 atgcggccgt gaatgtgatg aacggcaccg tcgacgtctg ctggggtggc ccgatgcggg
     8641 tgatgcaggc ttatcaggag atccccggct gtgacctcgt ctgcttcgcc gaggtggtga
     8701 cgcgtgatcc gttcctgctc atcgcccgcg agccccgccc cggcttttcc ctccgcgatc
     8761 tgctcggcct gcgcgtagcg accgtaagcg aagttccaac gccctggatg tgcctgcagg
     8821 aagaccttcg ccgcgcagga atcgatccgg ccgcactgtc gcgggtggcc gatcgcacaa
     8881 tggccgagaa cgtcgcggcg ttgacgcgcg gcgatgtcga tgtggtgcag gtgttcgaac
     8941 ccttcgcctc gagcggcggt ttcccggtgt ggtacgcgca ggcggatcgc ggagcgacct
     9001 cctacacgac attctatgcg cgacgcgggc tgctggccgc gcggcatgat gagctgctgc
     9061 gcatggtgcg ggcaatccat cgaacgctgg gttgggtggc agaggcggat ggcaacgcaa
//
LOCUS       DATGEM010000618        10857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_384086, whole genome shotgun sequence.
ACCESSION   DATGEM010000618 DATGEM010000000
VERSION     DATGEM010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384086"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..1635
                     /locus_tag="VKQ27_19085"
     CDS             451..1635
                     /locus_tag="VKQ27_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HLY91096.1"
                     /translation="MSLANDPSTAIRDATQRIEPGLIEIRRDIHAHPELAFEEIRTAG
                     VVARELERLGIEHQTGIARTGVVGLIKGGRPGPVLAIRADMDALPIEEKTGLPFASTN
                     EGLMHACGHDIHTTTLLGVAAVLKDLAPQLAGTVKLVFQPAEEAIGGMKEMIAAGVMD
                     NPKIDMALSLHNGPELPVGTFGFVRGHALAAADGFSITVRGKSGHAAHPHFAIDPIVA
                     AATLVTQLQTVVSREVRPIHPAVVTVGMIHGGTARNIIHDSCTITGTVRTLHPEARDT
                     AEAAIKRLAAGMLASMRVTCDVEYQRGVPSLRNDDRVVDPTIAAVRHQLGDVISEFSP
                     SLGGEDFALMAELVPAFQLRVGSGAPGRTDRLHNSGYQPDERCIGLGVQALSRATLEL
                     LA"
     gene            1640..2611
                     /locus_tag="VKQ27_19090"
     CDS             1640..2611
                     /locus_tag="VKQ27_19090"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLY91097.1"
                     /translation="MRICIYGAGSVGGYLAASLAQGGADVSVVARGPHLAAIQANGLT
                     VETPEGSITARLAATADPADLGLQDAVIVTVKSPALPAVAASITPLLGPDTPVAFVMN
                     GIPWWYFHPYEDRRIPRLDPGDALWNTIGPHRAIGGVFWPACSVPAPGIVRLLSGAGR
                     GTTFGEPDGSISPRIDAIAAAFRAASLPVTIAPNIRELIWQKLAFNLSAGPMCILTQS
                     PVRATHEESALIDCSRRLVAEAEAISAAMGYALNIDIDRVVATNTQLAHRPSILQDLE
                     AGRPMEIDALYGVPLELARLHGIATPTLDLLVAMIKEKARAAGLYNR"
     gene            complement(2622..4016)
                     /locus_tag="VKQ27_19095"
     CDS             complement(2622..4016)
                     /locus_tag="VKQ27_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91098.1"
                     /translation="MHIRWRLAGLVLAAVAVLVVGRPAQALPDFAAQTGQPCTACHIG
                     GFGPQLTPLGRAFKIGGYTQRGGEGWASYVPLSGMVLTSFNNTSAGVPADQIAHHYAA
                     NSNFALDQVSAFLAGGIGEHSGGFVQFTYSNIPNASHLDNTDLRPYTTTFDVFGNELR
                     VGTTINNNPTVQDPYNSTFAWGFPYVASGLAPTPAASPILASGFNNNSIGYTVYAWYN
                     HSLYLEGGIYTTLGTWWLARLGNDFGVGSSQGVMPYVRLAYEWDWNAQAFHVGALYMQ
                     SNVNPVSGVFETDGSMGRDHYRDFAVDAGYQFLGDGTHIVTVQGIYTHEDQNLEGTTN
                     AFNNANGTTFGSTSSLNQIRMNVSYWYQNTYGVTLGWMNTWGPANPVLYTTGSDLTNS
                     ANGSPNSNAFIIEADWVPFGKPDSWMSPWANLKLGIQYTAYTEFNGGNKNYDGFGRNA
                     SDNNTLYIFAWMAF"
     gene            complement(4133..4519)
                     /locus_tag="VKQ27_19100"
     CDS             complement(4133..4519)
                     /locus_tag="VKQ27_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HLY91099.1"
                     /translation="MVDGAALRRITLAIGFAFASGGPVAQAADPAAGKSVFNSYCSIC
                     HSAQAGKNMVGPSLFGVVGRPTGSVAGFHYSPANQNAHLTWDQATLDKYLASPRTVIP
                     GTTMTYGGLKDDTKRADLIAYLATLK"
     gene            4604..5152
                     /locus_tag="VKQ27_19105"
     CDS             4604..5152
                     /locus_tag="VKQ27_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91100.1"
                     /translation="MKSVTVIPAIALVAATILCAAAGTSSAQPAPHAGDIPQSLRLEH
                     QDTLQQLTALSRRHGPVGDEARKALELFKKHIQREEAYILPPLTLLPLLADGKTSPDM
                     KWAIPMADRVKTERDEIFQEHTRITDAMNALAAAAQKAHDAAALDFARAAVADSLNDI
                     ELIEPMTIVIGDYLKAKLPANP"
     gene            complement(5155..5376)
                     /locus_tag="VKQ27_19110"
     CDS             complement(5155..5376)
                     /locus_tag="VKQ27_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91101.1"
                     /translation="MIGRLLNLYNPTGTLSGVTTIAIVIRVAAWFALNRSWHARTVVL
                     GRINVVAFVLLAVGFLLTFPPFMEVLQGR"
     gene            complement(5669..6622)
                     /locus_tag="VKQ27_19115"
     CDS             complement(5669..6622)
                     /locus_tag="VKQ27_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLY91102.1"
                     /translation="MISQREWKGLDTGLKPVFLGYDQTERLIAALLDRAMQWQPEAVV
                     GITRGGVVPATMAAGIMALPLAMVGFERAAGTTQWIGAPPAAGRVLLVDDGCSTGRTM
                     AAVREALLGEGRECLTLAVVHDPEVTTYIPDLSHAMRSLWRFPWERGEATPAGRALRA
                     TGAGPDRVAELPFYGLDLDGVFLPDVPDAVYLASIADAVERRHALEPLASLPFFASER
                     AVVITGRPEMDRERTQAWLARWGFGALPLECRPEHVEHTPDLVARYKADVATRWGCTH
                     FVESDAEQALRIAALAPHLVVSWWSAADARAWLIGVAAQPG"
     gene            complement(6646..7710)
                     /locus_tag="VKQ27_19120"
     CDS             complement(6646..7710)
                     /locus_tag="VKQ27_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91103.1"
                     /translation="MRTSRSEPRIVAHADWSIDPRKRWVAIARRERTSWQLAAPVPVG
                     DVTTFLARLRGMAAGGAVALGVDLPLGLPRAYAASLPERGFPHFLASLATRPAFFQVC
                     ATLAELTPDRPFYPARGIRGMTRAAHALALGLGGSADLSRACDRATAQRPAGAPLFWT
                     LGANQSGKAAIAAWQHMILPALTGGDVLRLWPFDGPYRSLLAPGKVALAETYPAEAMR
                     HLGIVLKGSKRRQADRAAVAAGLSTAMAGLRAAPDTDCRAAMADGYGADPAGEDRFDC
                     TLGVLCVLNVLAGNRPDTAPEDGWIRQWEGWVLGQTAMPLDWPLWAERSLQKETGASG
                     GTRPKVVLGNGQDVNPSPAN"
     gene            complement(7691..7891)
                     /locus_tag="VKQ27_19125"
     CDS             complement(7691..7891)
                     /locus_tag="VKQ27_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91104.1"
                     /translation="MTEGQRAYEAKRAAKAGMSLEKWLAAKERQQQAEERAKQKAVAE
                     AAKPAKKRGLLSRLLERAHKPL"
     gene            8028..8516
                     /locus_tag="VKQ27_19130"
     CDS             8028..8516
                     /locus_tag="VKQ27_19130"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91105.1"
                     /translation="MQHRFRPYLLSAAVALIAGCGTAAAQQPTQAQADAIRQSCRADY
                     QSHCASVPTGGSAALQCLRSNLASLSPGCQSAVTAAGGSTAAAPSTVAPQTTTRPAPP
                     PPPMPMREQAMMMRQSCGADFRTWCQGVGFGGGGALACLADHRESLSEPCREALGHMQ
                     GR"
     gene            complement(8524..10389)
                     /gene="ftsH"
                     /locus_tag="VKQ27_19135"
     CDS             complement(8524..10389)
                     /gene="ftsH"
                     /locus_tag="VKQ27_19135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLY91106.1"
                     /translation="MPAPKQNLSSSIWLIVAGLIVIAALGLFSNGGGNGGGSDVIPYS
                     QFQDYLTANKVKEVTVSGNTIRGSLTEKMPDGRTEFTTVQVPPDLAAQLAQHHVQYSA
                     STTDSGALGTLLSWILPPLLFVGIWMFASRAMMGGGGRGGMGGFGGGLLSVGRSRAKL
                     VAETDVKVTFADVAGVDEAKEELHEIIDFLKRPEEFTRLGAHSPRGILLVGPPGTGKT
                     LLARAVAGEAGVPFYSTNGAEFVEMFVGVGAARVRDLFEQARQSSPCIIFIDELDALG
                     RARGVNPMQGQDEKEQTLNQLLAEMDGFDRSVAIVILAATNRPEILDPALLRAGRFDR
                     QVLVDRPDKKGRIEILRIHLTNLKVSPDVRLEEIAAMTPGFTGADLANLANEAAVLAT
                     RRGAAVIVEADFGGAVERIIAGLEKKSRILIPRERRTVAYHEMGHALVALAIPGSDPV
                     QKVSIIPRGIGALGYTLQHPTEDRFLMSKQELEDKMTVLFGGRAAEMLINVDVSTGAS
                     DDIAKATDIARGMVLRFGMDEKLGPVAWDTEQGQFLNQPGNFWQQRRFSDETAHEIDL
                     AVRAHLEAARARAVAILQLNRPALDEGAASLLAHETLTADELPKVVVERQREAAE"
     gene            10594..>10857
                     /locus_tag="VKQ27_19140"
     CDS             10594..>10857
                     /locus_tag="VKQ27_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91107.1"
                     /translation="MATIIQLGNSANTATLSNVDTLTSGTGADTILFTGALTNASIDL
                     GTGSDTLTFGAFTNSATVANTETITGGTGADTVTLGSALTTAMA"
BASE COUNT         1912 a         3960 c         3161 g         1824 t
ORIGIN      
        1 ccatttcccc caggccaggc ccgcatttgc gaaatgcgat cgcccagccc cctcgccgcg
       61 cgcgtcattg cggattcctc ccgcaacccc ctatgctcac cccaaccaca agagagtccg
      121 catgagaggt ctaccgttcg ccatggtcag cggccccctg ttcgcccagg tggtgcgcga
      181 ggccaacgcc gatccacggc ggacctctgt gtgcaattgc tgcggtgccc acatgccgat
      241 acagcctgac cgagccgagc agaccgttcg ctgccccacc tgctcccgct ggcaacgcgt
      301 cgcaacaagc gaggaaaccc cctggcgcct ttctgccgcc tcagccgagg ccctccgccg
      361 cacccgccgc tggctccggc acctctgatc cttcgaaccc gccctgcaca ccacggcccg
      421 tttcccgagg ctgatcccaa aggctgatca atgtcccttg ccaacgaccc cagcacagcg
      481 atccgcgacg ccacgcagcg catcgaaccc ggcctgatcg agatccgccg cgacatccac
      541 gcccaccccg agctcgcctt cgaggaaatc cgcaccgccg gcgtcgtcgc gcgcgagctc
      601 gagcgcctcg gcatcgagca ccagaccggc atcgccagga ccggcgtcgt cggcctgatc
      661 aagggcggcc gtcccggccc cgtgctcgcg atccgcgccg acatggacgc gctgccgatc
      721 gaggagaaga ccggcctccc cttcgccagc accaatgaag gcctgatgca cgcctgcggc
      781 cacgacatcc acaccaccac gctgctcggc gtcgccgcgg tgctgaaaga cctggcgccg
      841 cagcttgcgg gcacggtgaa gctcgtcttc cagcccgccg aagaggcgat cggcggcatg
      901 aaagagatga ttgcagccgg cgtcatggac aatccgaaga tcgacatggc gctgtcgctg
      961 cacaacggcc cggagctgcc cgtcggcacc ttcggattcg tacgcggcca cgccctcgcg
     1021 gcagccgacg gcttcagcat caccgtgcgc ggcaagtccg gtcatgccgc gcatccgcac
     1081 ttcgccatcg accctatcgt cgccgcggcc acgctggtca cccagttgca gaccgtcgtt
     1141 tcccgtgagg tccgcccgat ccacccggcc gtggtcaccg tcggcatgat ccatggcggc
     1201 accgcccgca acataatcca cgacagctgc accatcaccg gcacggtccg cacgctgcac
     1261 cccgaggccc gtgacactgc cgaggccgcc atcaagcgcc tcgccgccgg catgctggcc
     1321 agcatgcgcg tcacctgcga cgtcgagtac cagcgcggcg tcccctcgct gcgcaacgac
     1381 gatcgcgtcg tcgatcccac cattgcggcg gtgcgccacc agctgggcga tgtgatcagc
     1441 gaattctccc cgagcctcgg cggcgaggac ttcgcactga tggccgaact ggtgcccgcg
     1501 ttccagttgc gcgttggttc cggtgccccc ggccgcaccg accgcctgca caactccggc
     1561 taccagccgg acgaacgctg catcggcctc ggcgtccagg ccctctcccg cgccactctg
     1621 gagctgctgg cctagccggg tgcgcatctg catctacggc gcgggttccg tcggcggcta
     1681 cctcgcggcc tccctcgccc agggcggcgc cgacgtgtcc gtggtcgcac gcggcccgca
     1741 tctggccgcc atccaggcca acggcctgac cgtcgagacg cccgagggct ccatcaccgc
     1801 ccgcctcgcc gccactgccg accctgccga cctcggcctg caggacgcgg tcattgtcac
     1861 ggtgaagtcg cctgccctcc ccgccgtcgc ggcctcgatc accccgctgc tcggccccga
     1921 caccccggtc gcctttgtga tgaacggcat cccctggtgg tatttccatc cgtacgagga
     1981 ccgccgcatc ccgcgtctcg accccggcga tgccctctgg aacaccatcg gcccccaccg
     2041 cgccatcggc ggcgtctttt ggcccgcctg ctccgtgccc gctcccggca tcgtgcgcct
     2101 gctcagcggc gccggccgcg gcaccacatt cggcgagccc gacggcagta tctcccctcg
     2161 tatagacgcg atcgccgccg ccttccgcgc cgccagcctg cccgtcacga tcgcccccaa
     2221 catccgcgag ctgatctggc agaagctcgc cttcaacctc agcgccggcc ccatgtgcat
     2281 cctgactcaa tcgccggttc gcgccaccca cgaggaatcc gcactgatcg actgctcgcg
     2341 ccgcctggtc gccgaggccg aggcgatctc agcggcgatg ggctacgcgc tgaacatcga
     2401 catcgaccgc gtcgtcgcca ccaacacgca gcttgcgcac cgccccagca tcctccagga
     2461 cctggaagcc ggccggccga tggagatcga cgcgctctac ggcgtgcctc tcgagctggc
     2521 gcgcctgcac ggcattgcca cccccacgct cgatctgctg gtcgcgatga tcaaggagaa
     2581 ggcccgcgcc gccggcctct acaaccgctg acccgcgtcc gtcagaacgc catccacgcg
     2641 aatatataca gcgtgttgtt gtcgctcgcg ttgcggccaa acccgtcata gtttttgttg
     2701 ccgccgttga actcggtata ggcggtatac tgtatgccca gtttcaggtt ggcccacggc
     2761 gacatccagg agtctggttt cccgaacggc acccagtcag cctcaatgat gaacgcattg
     2821 ctgttcgggc tgccgtttgc gctgttcgtc agatcggagc cagtcgtgta gaggaccgga
     2881 tttgccggtc cccacgtatt catccagccc agcgtgacac cataggtatt ttgataccaa
     2941 taggatacgt tcatgcgtat ctggttcaga ctggatgtcg acccgaacgt tgtgccgttg
     3001 gcattgttga acgcattcgt cgtgccctcg aggttctggt cctcgtgggt gtagatgccc
     3061 tgcacggtca cgatatgcgt gccgtctccc aggaattggt agccggcgtc aacggcaaag
     3121 tcgcggtagt gatcccggcc catcgatcca tccgtctcga acacgccgga taccggattg
     3181 acattggact gcatgtagag cgcgccgaca tggaaggcct gtgcattcca gtcccattca
     3241 tacgcaagcc ggacatacgg catcacccct tgcgaactgc cgaccccgaa atcattcccc
     3301 agtcgtgcga gccaccatgt gcccagcgtc gtgtagatcc cgccttcaag atacaggctg
     3361 tgattgtacc aggcatagac ggtgtagccg atggaattgt tgttgaagcc actcgccagg
     3421 attggcgagg ctgccggcgt tggcgcgagg cccgatgcca catacggaaa tccccaggcg
     3481 aacgtcgaat tgtacggatc ctggaccgtg ggattattgt ttatcgtcgt gcccacacgc
     3541 agttcgttgc cgaacacgtc gaacgtcgtc gtataggggc gaagatcggt gttatcgaga
     3601 tgcgatgcat tagggatgtt ggagtaggtg aactgtacga agccgcccga atgttccccg
     3661 atacccccgg cgaggaacgc actgacctga tccagcgcga agttactgtt tgccgcatag
     3721 tgatgcgcga tctgatccgc cggcactccc gcgctggtgt tgttgaatga tgtcagcacc
     3781 atgcccgaca gcggaacata cgatgcccag ccttcaccac ctcgttgggt atagccgccg
     3841 atcttgaaag cgcggccaag cggcgtgagc tgcggaccaa acccgccgat atgacacgct
     3901 gtgcacggct ggccggtctg cgccgcgaag tctggcaacg cctgcgccgg ccggccgacg
     3961 accagcaccg ccactgccgc caggaccagg cctgcgagcc tccatcgtat atgcacggcg
     4021 cgattccccc tctgccagct ctaacttttt cggctgagca tagtctgtct ttgatccagg
     4081 tcaaagaaat acgtaaattt cttgcggaaa ggttaaccgg cggcttgggg aattacttga
     4141 gcgtcgccag ataggcgata aggtcggcac gctttgtgtc gtccttcaac ccgccatagg
     4201 tcattgtcgt gcccgggatc accgtccgcg gcgacgcgag atatttgtcg agcgtggcct
     4261 ggtcccatgt cagatgcgca ttctgattgg ccggcgagta atgaaagccg gccaccgacc
     4321 cggtcggtcg cccgacgaca ccgaacaggc tggggccaac catgttcttc cctgcctgcg
     4381 cggaatggca gatcgagcag taggagttga acaccgactt gcccgcggca ggatccgctg
     4441 cctgcgcgac tggacctcca ctggcaaagg caaatccaat cgccagtgta atccgccgca
     4501 gcgccgcacc atctaccatg gctgcttctc cttccgattg agtcagcata acatctgtgt
     4561 attcatagca aaacagcttg tgcagtttgc gggagcaaat gccatgaagt cagtaaccgt
     4621 cataccggcc atcgcgctcg ttgctgccac catcctgtgc gccgccgctg gaacgtcgtc
     4681 agcgcaaccg gcgcctcatg cgggtgacat tcctcagtcg cttcgcctgg aacaccagga
     4741 cacattgcaa caactcacag cgctctcccg ccgccacggt cctgtcggcg acgaagcacg
     4801 caaggcgctc gaactgttca agaagcatat acagagagag gaagcctaca tcctgccacc
     4861 gctcacgctg ctgccgctgc tcgccgacgg gaaaacctcg cctgacatga aatgggcaat
     4921 cccgatggcc gatcgcgtga agacggagcg tgacgagata ttccaggaac acacccggat
     4981 caccgacgcc atgaacgcgc tcgccgcggc cgcccagaaa gcgcatgacg cggcggcact
     5041 cgactttgcc cgcgccgcgg tggcggattc gctgaacgac atcgaactga tcgaaccgat
     5101 gacgatcgtc atcggcgatt acctcaaggc gaagctgccc gcgaacccgt aacgctatct
     5161 gccttgcagc acctccatga acggcggaaa cgtcagcagg aaccccacgg cgagcaacac
     5221 aaacgccacc acattgatcc ttcccaggac aaccgtcctg gcatgccagg aacggttcaa
     5281 cgcgaaccac gccgccaccc tgatcacgat ggcaattgtg gtcacgccag acagtgtccc
     5341 ggtcggatta tagaggttga gcaggcgccc gatcatggga gatgcatcgg ccgaaaaggc
     5401 cagaacgccc aatgccgcgc agccgatacc aggcgatata ggcaccggta aatacggtaa
     5461 gccacgccag cagaaccatg ctcacagggt agatcgccag catcgtatat tcgcggctgg
     5521 ccgccagccc ctgaccccgt ggcgccgaca tgcggcagat ttgagcgagg gctctcgaac
     5581 gccagcatga acagggctcc cagacccacg ccatgcaaca ccgtgaacag atctcaggcg
     5641 gtgatctcca tgtcgtccct ccctctgttc agcccggttg agccgccacc ccaatcagcc
     5701 acgcccgggc atcggccgcc gaccaccaac tcaccacgag gtgcggcgca agtgcggcaa
     5761 tccgcagtgc ctgctcggcg tcgctttcca cgaaatgcgt gcacccccag cgtgtcgcaa
     5821 cgtcggcctt gtagcgcgcc accaggtccg gcgtgtgctc cacatgctcg ggccgacact
     5881 ccagtggcag cgccccaaac ccccagcgcg ccagccacgc ctgcgtccgc tcccgatcca
     5941 tctcgggacg ccccgtgatc acgaccgcgc gctccgacgc gaagaacggc aggctggcca
     6001 acggttccag cgcgtgccgg cgctcgaccg catcagcgat ggacgccaga tacaccgcgt
     6061 ccggcacgtc cggcaggaac accccatcga gatccagccc atagaacggc agttccgcca
     6121 cgcgatccgg tcctgccccg gtggcacgca gcgcgcgacc ggctggcgtc gcctcgcccc
     6181 gctcccaggg gaaacgccat agcgaccgca tggcgtgtga tagatccggt atatacgtgg
     6241 tcacctcggg atcgtgcacc accgccagcg tcaggcactc ccgcccctcg cccagcagcg
     6301 cctcgcgcac cgcggccatc gtgcggccgg tgctgcatcc gtcgtccacc agcaggacgc
     6361 gcccagcagc cggcggagcg ccgatccatt gcgtcgtacc agccgcgcgt tcaaagccca
     6421 ccatcgccag cggcagcgcc atgatcccgg cagccatcgt cgcgggcacc acgccgccgc
     6481 gcgtgatacc caccacggcc tcgggctgcc actgcatcgc gcgatccagc agcgcggcaa
     6541 tcagccgttc cgtctgatcg tagccgagga acaccggctt aaggccggta tccaggcctt
     6601 tccattctcg ttgcgatatc aagtcattaa ctttcttgcg acttgtcaat ttgctggcga
     6661 tgggttaacg tcctgaccgt taccgagaac aacctttggg cgcgtcccgc cagacgcgcc
     6721 cgtttctttt tgtaggcttc gctctgccca caacggccaa tccagcggca tcgcggtctg
     6781 gcccagcacc catccttccc attgcctgat ccacccgtct tcgggcgctg tatccggccg
     6841 gttgccggcg agcacattca gcacgcatag cacacccagc gtgcaatcga atcggtcctc
     6901 acccgccggg tcggcgccat acccgtcggc cattgccgcc cggcagtctg tatcaggcgc
     6961 ggcgcgtagt cccgccatcg cggtggacag accagcggcc actgccgcgc gatcagcctg
     7021 gcgccgcttg ctgcccttca gcacaattcc cagatgccgc atcgcctccg ccggataggt
     7081 ctctgccagt gccaccttcc caggcgccag caggcttcga taaggaccgt caaacggcca
     7141 caaccggaga acgtcgcccc cggtaagggc cggaagtatc atgtgctgcc atgctgcgat
     7201 cgcggccttt cccgactggt ttgcgccgag ggtccagaac agcggcgccc cggccggacg
     7261 ctgcgcggtc gcgcgatcgc aggcacgcga tagatccgcc gaaccaccca gacctagcgc
     7321 cagtgcatgc gccgcccgcg tcatccccct gatgccgcgc gcgggataga aggggcggtc
     7381 cggtgtcagt tcggcgagcg tcgcgcagac ctgaaagaac gccggccggg tcgccaggct
     7441 ggccaggaaa tgcgggaagc cgcgctccgg cagactcgct gcataggcgc gaggcagccc
     7501 cagtggcaga tcgaccccga gcgcgacggc gccaccggcc gccattccgc gaagccgcgc
     7561 caggaatgtg gtcacatctc ccaccgggac cggtgccgca agctgccagc ttgtcctctc
     7621 gcgccgggca atcgccaccc agcgcttgcg cggatcgatg ctccaatccg cgtgcgcgac
     7681 gatccttggc tcagagcggc ttgtgcgcac gctccagcaa ccgggacagc agcccgcgct
     7741 ttttcgccgg cttggcagct tccgctaccg ccttctgttt ggcgcgctct tcggcctgct
     7801 gctgccgctc cttcgccgcg agccacttct ccaggctcat gcccgccttc gcagcgcgct
     7861 tcgcttcata ggcccgctgg ccttcggtca atgtccgttg ggtcttcaag gtaatccccc
     7921 gctcgcgccg cttttcgcag gcgtgcggcg atggttcaag ggcctgtgat tgttccggca
     7981 caccatggta ggcaagctcg gtgcttcaca ttgtgagcga gtccgcaatg caacatcgat
     8041 tccgccccta tctactatcc gcagctgtcg cactgatcgc cggctgcggc accgcggcgg
     8101 cacagcagcc gacgcaggct caggccgacg caatccgcca gtcctgccgc gccgactacc
     8161 aatcgcactg cgccagcgtg ccaaccggag gctcggccgc tctgcagtgc ctgcggagca
     8221 accttgcgag cctgtcgccg ggctgccaga gcgcggtgac cgccgcgggc ggttcgaccg
     8281 ctgccgctcc gtcgacagta gcgccgcaga ccaccacaag gcccgccccg ccgccaccac
     8341 cgatgcccat gcgcgagcag gccatgatga tgcgccagtc atgcggcgct gacttccgca
     8401 cctggtgtca gggtgtggga tttggtggtg gcggggcgct ggcctgcctt gccgatcacc
     8461 gcgagagcct gtcagagccg tgccgcgagg cgctgggtca tatgcaagga cgataaaggc
     8521 agcctattcg gctgcctcgc gctgccgctc caccacgacc tttggcagct catccgccgt
     8581 caatgtctca tgcgccagca atgacgccgc gccctcatcg agcgcgggcc gattgagctg
     8641 caggatggcg actgcccgcg cccgcgccgc ctcaagatgt gcgcgcaccg ccaagtcgat
     8701 ctcgtgcgcc gtctcatccg agaacctccg ctgctgccag aaattgccag gctggttgag
     8761 gaactgcccc tgctcggtat cccacgccac cggccccagc ttctcgtcca tgccgaaccg
     8821 cagcaccatg ccgcgggcaa tgtcggttgc cttggcgata tcgtccgatg cgccggtgga
     8881 cacgtctaca ttgatcagca tttccgccgc gcggccgccg aacaggacgg tcatcttgtc
     8941 ctccagctcc tgcttgctca tcaggaaccg gtcctcggtc gggtgctgca gcgtatagcc
     9001 aagcgcgccg atgccgcgcg ggatgatcga gaccttctgc accggatcgc tgcccgggat
     9061 cgccagcgcc accagcgcgt gccccatctc gtggtaggca acagtacgcc gctcacgtgg
     9121 gatcaggatg cgcgatttct tctcgagccc cgcgatgatc cgctccacag cgccgccgaa
     9181 atcggcctcg acgatcaccg cggcgccacg ccgggttgcc agcaccgccg cctcgttggc
     9241 caggttcgcc agatcagcgc cggtgaagcc cggcgtcatc gccgcgatct cctcgagcct
     9301 cacgtccggc gataccttca ggttcgtcag gtgaatgcgc aggatctcga tgcgcccctt
     9361 tttgtcaggc cgatcgacca gcacctggcg gtcgaagcgt ccggcccgca gcagcgccgg
     9421 gtccaggatc tccggacggt tggtcgccgc gaggatgacg atggcaaccg agcggtcgaa
     9481 cccatccatt tcggcaagca gctggttgag cgtctgctct ttctcgtcct ggccctgcat
     9541 cggattgacg ccgcgcgcgc ggcccagcgc gtccagttcg tcaatgaaga tgatgcaggg
     9601 cgacgattgc cgcgcctgct cgaacaggtc gcgcacgcgg gcggctccga cgcccacgaa
     9661 catctcgacg aactcggcgc cgttggtcga gtagaacggc acgcccgcct cgcccgccac
     9721 ggcgcgcgcc aacagggtct tgccggtgcc gggtggtccc accagcagga tgccgcgcgg
     9781 gctgtgcgcg cccagccggg tgaactcctc cggccgcttc aggaaatcga tgatctcgtg
     9841 cagttcctcc ttcgcttcgt ccacgccggc gacgtcggcg aaggtcacct tcacgtcggt
     9901 ctccgccacc agcttggcgc gggaccggcc caccgacagc aggccaccgc cgaagccacc
     9961 catgccgcct cgcccgccac cacccatcat cgcgcgcgag gcgaacatcc agatgccgac
    10021 gaatagcagc ggcggcagga tccaggacag cagcgttccc aaagctccgc tatccgtggt
    10081 gctcgcactg tactgcacgt gatgctgggc gagctgcgcg gccaggtcgg gcggcacctg
    10141 gacggtggtg aactcggtcc ggccatccgg catcttctcg gtcagcgatc cgcgtatcgt
    10201 gttgccggag accgtgacct ccttgacctt attggctgtc aggtagtcct ggaactggct
    10261 gtaggggatc acgtctgatc cgcccccatt gccaccgcca ttggagaaca gcccgagcgc
    10321 ggcaatcacg atcagccccg cgacaatcag ccagatcgag gaggacaggt tctgcttcgg
    10381 ggcaggcacg cgcgcgttct cccgctagac aaaagctgaa catgggccgg cccacgacgc
    10441 ccggcaacca gccgcgcggc ccgtgcgggt cgggcgacaa ccaattcttc acaagttcag
    10501 tgtattggtt ctgttcgcgc aactgcgcga tttcgccagg ccgcgggaca aaacggccga
    10561 tcccctggtg cacccggagc ctggagatcg gccgtggcaa caatcatcca acttggcaac
    10621 tccgcgaaca cggcaacgct cagcaatgtc gacacgctga cgtctggcac cggcgctgac
    10681 accatcctgt tcaccggcgc cctcaccaac gcctcgatcg atctcggcac cggcagcgac
    10741 acactcacct tcggcgcctt caccaactcc gcaaccgtcg ccaacaccga gaccatcacc
    10801 ggcggcaccg gcgccgacac cgtcaccctc ggctccgccc tcaccaccgc catggcc
//
LOCUS       DATGEM010000619         9459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1619325, whole genome shotgun sequence.
ACCESSION   DATGEM010000619 DATGEM010000000
VERSION     DATGEM010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619325"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VKQ27_19145"
     CDS             <1..1006
                     /locus_tag="VKQ27_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLY91108.1"
                     /translation="AWSGIAGLSYRNAEGAIQHNEERAVLENMDALPFVTPVYKRDLV
                     IENYYIGYLKHPYVSIYTGRGCPARCTFCLWPQTVGGHRYRTRSVGHVVEEIALAKRY
                     FPQVREFFFDDDTFTDNLPRAEAIAKELGKLGVTWSCNAKANVPRDTLKVLKDNGLRL
                     LLVGYESGNQQILHNIKKGMRIEVAERFTRDCHDLGIKIHGTFILGLPGETQQTIEET
                     IRFATKINPHTIQVSLAAPYPGTYLHRQAVENGWLDETHAELVDEHGIQIAPLTYPHL
                     SHTEIFDNVETFYKRFYFRAPKIAAIVGEMVRSPQMMKRRLREGVEFFQFLRERHTAA
                     "
     gene            1069..1908
                     /gene="hpnK"
                     /locus_tag="VKQ27_19150"
     CDS             1069..1908
                     /gene="hpnK"
                     /locus_tag="VKQ27_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HLY91109.1"
                     /translation="MRRLTFTADDFGLTSSVNEAVERAHRAGALDAASLMVAAPAAAD
                     AVRRARDLPTLEVGLHLVVIEGPAVLPPSRIPDLVDADGQFPSDQLRLGVNYFARPHI
                     RRQLAAEIRAQFAAFAATGLPLSHADAHKHMHLHPTVGRLMVEIGREFGLDRVRVPAE
                     PPGILARCSTKPGLGGHALYHWSRLLRRQVRAAGMTTTDHCFGVAWSGHMTHERITRL
                     LAELPAGTSEIYCHPAIERDATLRALMPDYEHEAELAALLALPPYRTGGVALANQVKF
                     PAI"
     gene            1933..3309
                     /locus_tag="VKQ27_19155"
     CDS             1933..3309
                     /locus_tag="VKQ27_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLY91110.1"
                     /translation="MRRLLFCAAVAFVAAVMPMTPASARSMDSADARIAQNATPAVVS
                     ISLWKVRDATTPGGTPRRVKVYGSGFIIDPSGIVVTNKHVIDGAISAQVVLSDGTPYT
                     AKVLGVAAMVDIAVLKIDASHPLPILKWGNSDALKVGDAVLTMGNGLAIGLSVSAGIV
                     SALNRDLQDSPFDSYIQTDAAINHGNSGGPLIDADGDVIGIDTALYNPDQNGGFIGIG
                     FAIPSSTAKFVVDHLLDPHHSKPGWLGFALQDMTPELAQALGVQRRGGSIISVINAGG
                     PASKAGLRTGDVLEAINEDHPADARAFMRAIVVLPIDSQAKLTIWRDGKDQTITATIA
                     EWPNLMPQGGMMTGKMAAMMVQKAPDPGVKLALITDAARKQYGLDPKLNGVLISSVEA
                     GTEASDLGIVPGDVITSIQGTPVTTPDEVKHAVMAAHEQHRPYLAVLIKSKDVARWVS
                     ISIGPATS"
     gene            complement(3388..3744)
                     /locus_tag="VKQ27_19160"
     CDS             complement(3388..3744)
                     /locus_tag="VKQ27_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91111.1"
                     /translation="MRLTSKMAFAALLLGSTFGMTTLAAHAATPGCPSATQKTEAEGG
                     AGGNWQGKPGAATQKTEAEGGAGGNWQKQASATQKTEAEGGAGGNWQGKPGASVQKTE
                     AEGGAGTQQASAQPCK"
     gene            complement(3914..6727)
                     /locus_tag="VKQ27_19165"
     CDS             complement(3914..6727)
                     /locus_tag="VKQ27_19165"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HLY91112.1"
                     /translation="MLSPVTQLPPPETSVSALTAALAALTEDGAPVSRDAALGAFRRH
                     LARIQQHVQHTFEQEQITGLQAARLLGKLTDGLITALYEHATSDTGLGEPEHLSVAGT
                     GGYGRGVLAPFSDIDLLFLTAHEPSRQTLQVVEYMLYFLWDLGVKVGHATRSIEDCLV
                     EGAKDTTIRTALLDARHVTGNAKLFGEFHTKFRAACKEAGVADYIAAKQAERDVRHRR
                     YGDSPFVVEPNVKEGRGGLRDLQTLYWIARYVYDTQTMGELADVGDVLTPAEAKHGRR
                     AWEFLWTVRFHLHYVAGRAEERLTFDLQPVVGARMGYTRHGRQDGVERFMRHYFLTAR
                     EIARLTRILEPAILREALGPPAIAAETDQTLLKCGFLLAEGKLLPLPGRDFDKEPLQM
                     LSMLQVARDRSLELHPLAIRSLIQNERRAVELRGDPKAAALFMDLMCGKEPDGHRASP
                     HPDGARWLSILNEAGFLGRFIPDWARIVGQMQFDTYHVFTVDEHTIEAIRILNTLERG
                     ELAEVAPIASGLVDHLQSRRALYLAMLLHDIAKGRGGDHSEIGAEIALKVGPELGLTA
                     EETETVSWLVLHHLLLSGVAFKRDIDDPKTILDVADTVQSPERLRLLLVLTVADMRAV
                     SPKVWNGWKATLLRELYGRVAEVLAGGLSTTERDVRVDRARHAAAMLLTDWSKEDIER
                     FTELGYPGYWLSFDPETHARHARLVRDAEARKAPLTVDTQPLPARAVTEVTVYCADHA
                     GLFSKISGALAVAGGSIVDARIHTMTNGMALDTFWIQDAAGGAFDAPHRLARLAVLIE
                     QALSGRIRLGVEIRKMSKVLLGRRLRSIHVPPRVVIDNHASNTYTVLEVNGRDRPGLL
                     HDVTAAISEQGVQIASAHVTTYGVRAVDVFYVKDVFGLKIENERKLAALREALLKALE
                     NPEDAAPIQALPTRKRRRVAAGA"
     gene            complement(6741..9380)
                     /gene="mutS"
                     /locus_tag="VKQ27_19170"
     CDS             complement(6741..9380)
                     /gene="mutS"
                     /locus_tag="VKQ27_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLY91113.1"
                     /translation="MAASFRSTPNAAGATPAMAQWFAAKEAHPDALVFFRMGDFYELF
                     FSDAEAAAAALDIALTQRGEHNGTPVPMCGVPQHAAEAYLARLIRRGFRVAVTEQMED
                     PKSRTGKAPIKREVVRLVTPGTITEEALLEAGRANLLLGLALTRDEVGAAWLDVSTGL
                     FETMALQAKDLPGLLGRLEPVEVLAPDGLALGDWAEKRAPDVAPSPPLVARRRLAEVF
                     GVASLDAFGSFSDAEAIAALMAVDYVRATQAGTLPRLAHPMPQGSAGLLAMDAATRTS
                     LEIHRARDGGGMHSLLGAVQRTLTPAGARLLANWLAAPLTDPMAVAARQDAWGWLLAE
                     RTAADGLRTALRTAPDIARALARLSVGRGTPRDLAAMRDGLRAAGGALSCVTANGARS
                     LPSLLGSAIGALCLDPSLEQTLADALAEPAPHRLDDGNAIRPGFDPELDAERALRDDS
                     RRVLATLQLDYAQRYGVASLKIKHHAQLGYVIEAPAAAVEKLRDYPELTLRQGMANGA
                     RFTTPELSDLDRRISEAGERAAVRERAVFAQLVQSTLSQADALAACADALALMDALQS
                     AAKLAEPGTWCRPLVTGDDAFLVKAGRHAVVEAALAGHAQFVPNDCDLSPEQRVLLLT
                     GPNMAGKSTFLRQNALMVVLAQSGLPVPAESATIGVVDRLFSRVGAADDLARGRSTFM
                     VEMTETAAILHQAGPRSLVVVDEIGRGTATLDGLAIAWAVLESLHSANRCRTIFATHF
                     HELAELAERLPRIKPHTMRVKEWKGTVVFLHEVAAGAAGRSWGVHVAELAGVPAPVVR
                     RAASLLATLEKQRGPANAPLATLPLFAAAPSPAPEDAPSPEPNLLAEAVADLEPDRMT
                     PRDALDALYRLKAILGVSDAAAN"
BASE COUNT         1678 a         3170 c         2966 g         1645 t
ORIGIN      
        1 cgcctggagc gggatcgccg gcctctccta tcgcaacgcc gagggcgcca tccagcacaa
       61 cgaggaacgg gcggtcctgg aaaacatgga tgcgctccct ttcgtgacgc cggtctacaa
      121 gcgcgacctc gtcatcgaaa attactacat cgggtacctc aagcatccct acgtctccat
      181 ctacacggga cgcggctgcc cggcgcgctg caccttctgc ctctggccgc agacggtggg
      241 cggccatcgc taccggacgc gctcggtcgg ccatgtggtg gaggagatcg cgctggcgaa
      301 acgctacttc ccgcaggtgc gcgaattctt cttcgacgac gacaccttca ccgacaacct
      361 gccgcgcgcc gaggcgatcg cgaaggagct cggtaagctg ggcgtcacgt ggtcgtgcaa
      421 cgccaaggcc aatgtgccgc gcgacacgct gaaggtgctg aaggacaacg gcctgcgcct
      481 gctgctcgtc ggctacgaga gcggcaacca gcagatcctg cacaacatca agaagggcat
      541 gcggatcgag gtggcggaac gcttcacgcg cgactgccac gacctcggca tcaagatcca
      601 cggcaccttc atcctcggcc tgcccggcga gacgcagcag acgatcgagg agacgatccg
      661 cttcgctacc aagatcaacc cgcacacgat ccaggtatcg ctggccgcgc cgtatcccgg
      721 cacgtatcta caccgccagg cggtggagaa cggctggctg gacgagacac atgcggaact
      781 ggtggacgag catggaatcc agatcgcgcc gctgacgtat ccacacctgt cgcacacgga
      841 gatcttcgac aacgtcgaga ccttttacaa gcgcttctac ttccgcgcgc cgaagatcgc
      901 ggcgatcgtg ggagagatgg tccgcagccc gcagatgatg aagcgccgcc tgcgtgaggg
      961 cgtggagttc ttccagttcc tgcgcgagcg gcacacggcc gcgtaggcac cgcgctggcg
     1021 gggtgcgacc gaggcgaaga cgagctggcg gcagacatac gatcgcctat gcggcgcctc
     1081 acctttaccg cggacgattt cggcctgacc tcgtcagtca acgaagccgt cgagcgcgcc
     1141 caccgcgccg gcgctctgga cgctgccagc ctgatggtcg ccgcgccggc cgccgccgat
     1201 gcggttcggc gtgcccgcga tttgccaact ctcgaggtcg gactgcacct cgtggtcatc
     1261 gaagggccgg ctgtcctgcc gccatcccgc attcccgacc tggtcgatgc cgacggccag
     1321 tttccatccg accagctgcg gcttggcgtg aactacttcg cgcgcccgca catccgccgc
     1381 cagctcgccg ccgaaatccg cgcgcagttt gctgcattcg ccgcgacagg cttgccgctc
     1441 tcccacgccg atgcgcacaa gcacatgcat ctccacccca ccgtcggccg gctgatggtc
     1501 gagattggcc gcgagttcgg gctcgaccgc gtgcgtgtcc cagccgaacc gccgggaata
     1561 ttggcgcgtt gcagcacgaa gcctggcctc ggcggccatg ccctctatca ctggtcgcgc
     1621 ctgcttcgcc gtcaggtgcg cgccgccggc atgaccacca cggaccactg cttcggcgtc
     1681 gcctggagcg ggcacatgac gcacgaacgc attacccgcc tgctggcgga acttcccgcc
     1741 ggaaccagcg aaatctactg tcacccggcg atcgaacgcg acgcgacgct gcgtgcgctg
     1801 atgccggact acgaacatga agcggagttg gctgcactgc tcgccctgcc gccgtaccga
     1861 accggtggcg ttgcgctggc aaaccaagtt aaattccccg ccatctgaat aacggagaga
     1921 gggggaggcc aaatgcgccg cttgctgttc tgcgccgccg ttgcatttgt cgctgccgtc
     1981 atgccaatga cgcccgccag cgctcgatcg atggacagcg cagacgcccg gatcgcgcaa
     2041 aatgctacgc cggctgtcgt cagcatatcg ctttggaagg tgcgcgatgc caccacgcct
     2101 ggaggcacgc ctcgtcgcgt gaaggtctac gggtccggct tcatcattga cccgagcggt
     2161 atcgtcgtga ccaacaagca tgtgattgac ggcgcgatca gcgcgcaggt cgtgctgagt
     2221 gatggcactc cctacacggc caaggtgctt ggtgttgcgg cgatggtcga tatcgctgtg
     2281 ctgaagattg atgccagcca ccccctgccc atcctcaaat ggggcaacag cgacgcgttg
     2341 aaggttggtg atgcggtgct gacgatgggc aacggactcg ccattggatt gtcagtgtct
     2401 gctggcattg tcagtgcgct gaaccgcgac ctgcaggact cgccgttcga cagctacatc
     2461 cagacggatg ccgcgatcaa ccatggcaat tccggcggtc ctttgatcga tgcagatggt
     2521 gacgtgattg gcattgacac tgccttgtac aaccccgatc agaatggcgg cttcatcggc
     2581 atcggctttg ccatcccgtc gagcacggcg aaattcgtcg tcgaccacct gcttgatccg
     2641 caccattcga aaccaggctg gcttggcttc gcgctacagg acatgacacc cgagctggcg
     2701 caggcgctcg gcgtacaacg ccgcggaggc tcgatcatct cggtgatcaa cgccggaggc
     2761 cccgcgagca aggcggggct gcgcacgggt gatgtgctgg aggcgatcaa cgaggaccat
     2821 ccggccgacg cacgtgcctt catgcgcgcg atagtcgtct tgcccatcga ctcgcaggcg
     2881 aagctgacga tatggcgcga tggcaaggat cagaccatta cggccacgat cgcggaatgg
     2941 ccgaacctga tgcctcaggg cggcatgatg accggcaaga tggccgccat gatggtgcag
     3001 aaggcgccag atccaggcgt gaagcttgcg ctgattacgg atgcggcgcg caagcagtac
     3061 ggccttgacc cgaaactgaa cggtgtcctg atctcgtcag tggaagccgg taccgaggca
     3121 agcgatctcg gcatcgttcc gggcgatgtg atcacgtcga tacaaggcac gcctgtcacc
     3181 acgcccgatg aagtaaagca cgccgtcatg gccgcgcatg agcaacaccg gccgtatctc
     3241 gcagtgctta ttaaaagcaa ggacgtcgca cgctgggttt cgatttcaat cggcccggca
     3301 acaagctgaa aacagcagcg gccggcggtt ggtaacccgc cggccgctgt gcaatgcatc
     3361 aggccgccac agcggcccgg cagactgtta cttgcagggc tgcgcagagg cctgctgggt
     3421 gccagcgccg ccctcagcct cggtcttctg cacactggcc ccaggcttcc cctgccaatt
     3481 gccaccagcg ccgccctcag cctcggtctt ttgcgtagct gatgcctgct tctgccagtt
     3541 gccgccggca ccgccctcag cctcggtctt ctgcgtcgca gccccaggct tcccctgcca
     3601 attgccgcca gcgccgcctt cggcttcagt cttctgtgtg gccgacgggc agcccggcgt
     3661 ggccgcatgt gcggcgagag tcgtcatacc gaaagtcgag ccgagcagaa gcgcggcgaa
     3721 cgccattttc gatgtcaggc gcatagtaga cctccttggt ttggtcggct gtgaaagaca
     3781 tttaacttgc ttgtagaccg cgggcaacca cagccattag tcgctgctgt caaggctaat
     3841 gactccgcaa cgccgaaaat gtttcccacc aacttgcaat tttcgactgt ttttttgtga
     3901 tcgagctaac tgttcaggcc ccagcggcca cacggcgcct cttgcgggtg ggcaatgcct
     3961 gtatcggggc agcgtcttcg ggattctcca acgccttcaa cagcgcctcc cgcaacgcag
     4021 caagcttgcg ctcgttctcg attttcagac cgaacacatc cttcacatag aatacgtcca
     4081 ccgcgcgcac gccgtaggtc gtgacatgcg ccgaggcaat ctgcaccccc tgctcgctga
     4141 tcgccgctgt cacatcatgc agcagccccg gccgatcgcg cccgttcact tccaggacag
     4201 tgtaggtgtt ggatgcgtgg ttatcgatca ccacgcgcgg cggcacatgg atagatcgta
     4261 atcttcgccc gagcagcacc ttcgacatct tgcggatttc gacgccgagt ctgatgcggc
     4321 cggagagcgc ctgctcgatc agcaccgcca gccgcgccag ccgatgcggt gcatcgaacg
     4381 caccaccggc ggcatcctgg atccagaacg tgtcgagcgc catgccattg gtcattgtat
     4441 ggatgcgtgc atccacaatg gagccgcccg ccacggccag ggcacctgaa atcttgctga
     4501 acaaccctgc atggtcggcg caatagaccg tcacttcggt gacggcgcgc gctggcaatg
     4561 gttgcgtatc cacagtcagc ggcgccttgc gcgcctctgc atcgcgtacc aaccgcgcat
     4621 gccgcgcatg tgtttccgga tcgaacgaca gccagtagcc gggatagccg agttcggtga
     4681 accgctcgat gtcctccttc gaccagtcgg tcagcagcat tgccgctgca tgccgcgcac
     4741 ggtcaacgcg cacgtcacgt tcggtggtgg acaggccgcc ggccaacacc tcggcgacac
     4801 gcccgtagag ttcgcgcagc agcgtcgcct tccagccatt ccataccttc ggtgataccg
     4861 cgcgcatgtc ggcgactgtc agcaccaaca gcaagcgcag ccgctcaggc gactgcacgg
     4921 tatcagcgac atccagtatc gtcttcggat cgtcgatatc gcgtttgaac gcgacaccgg
     4981 acagcagcag gtggtgcagc accagccagg acaccgtctc ggtctcttct gccgtgagcc
     5041 caagctctgg cccgaccttc agcgcgatct ccgcgccgat ctccgagtgg tcgcctccgc
     5101 gtcccttggc aatatcatgc aacagcattg ccagatacaa cgcgcgccgc gattgcagat
     5161 gatcgacgag gccgctagcg attggcgcta cttcggccaa ttccccgcgc tcgagcgtgt
     5221 tcaggatgcg gattgcctcg atcgtgtgct catcgacggt gaacacatga tacgtgtcaa
     5281 actgcatctg cccgacgata cgcgcccagt ctgggatgaa ccgccccagg aaccccgcct
     5341 cgttcaggat ggaaagccag cgagcgccat caggatgcgg actggcacga tgaccgtccg
     5401 gctccttgcc gcacatcagg tccatgaaca gcgccgcggc tttcggatcg ccgcgtagct
     5461 ccaccgcgcg ccgctcattc tggatcagcg agcggatcgc gagcggatgc agttcgaggc
     5521 tgcggtcgcg agccacctgc agcatgctca gcatctgcag cggctccttg tcgaagtcac
     5581 gccccgggag cggcagcagc tttccctccg ccagcaggaa cccgcatttc agcagcgtct
     5641 gatccgtctc agcggcgatc gccggcggac ccagtgcctc acgcaggatc gccggttcca
     5701 ggatgcgggt cagccgcgcg atctcgcgtg cggtgaggaa ataatggcgc atgaaccgtt
     5761 cgacgccgtc ctggcggcca tgacgcgtgt agcccatgcg cgcgcccacc accggctgga
     5821 gatcgaaggt cagccgctcc tcggcgcggc cggcgacgta gtgcaggtga aaccggacgg
     5881 tccacaagaa ttcccaggcg cgacggccgt gctttgcttc tgctggcgtc aatacatcac
     5941 cgacatcggc gagctcgccc atcgtctgag tgtcgtagac gtagcgcgcg atccagtaca
     6001 gcgtttgcag gtcacgcagg ccgccgcggc cttccttcac gttgggctcg acaacaaatg
     6061 ggctgtcgcc gtagcgacga tgccgcacat cgcgctcggc ctgctttgcg gcgatgtagt
     6121 cggcaacgcc ggcttccttg cacgccgcgc ggaacttcgt gtggaactcg ccgaacagct
     6181 ttgcattgcc ggtgacgtga cgggcgtcga gcagcgctgt gcggatcgtg gtgtccttcg
     6241 cgccttcgac caggcaatcc tcgatcgagc gcgtcgcgtg cccgaccttc acgcccagat
     6301 cccacaggaa gtagagcatg tactccacca cctgcagagt ttgtcgcgat ggttcatgcg
     6361 cggtcaggaa cagcagatcg atgtcgctga atggcgccag cacgcctcgc ccgtaaccgc
     6421 cggtgcccgc tacggagaga tgttcaggct cgccaagacc ggtgtcggat gtggcgtgct
     6481 cgtaaagcgc ggtgatcaac ccgtcggtga gcttgcccag cagcctggcc gcttgcagcc
     6541 ctgtgatctg ctcttgctcg aacgtgtgct gcacgtgctg ctggatgcgt gccagatgcc
     6601 ggcgaaaggc gcccagcgcg gcgtcgcgcg acaccggcgc gccatcctcg gtcagcgcgg
     6661 cgagcgcggc ggtcagtgcc gaaaccgacg tttcgggtgg cggaagttgg gtaacggggc
     6721 tcagcatcca gacaaacgta ctagtttgct gccgcgtcgg aaactcccag tatcgctttc
     6781 aggcgataca aggcgtccag agcgtcgcgc ggggtcattc gatccggttc caggtcggca
     6841 accgcttctg ccagtaggtt cggttcggga gatggcgcat cctctggtgc aggagaaggt
     6901 gccgcagcga atagcggcaa tgtggccagc ggggcattgg ccggcccgcg ctgcttctcg
     6961 agtgtcgcca gcagcgaagc agctcggcgt accaccggcg caggcacgcc agcaagttcc
     7021 gcgacatgca cgccccagga gcggccggca gcaccagctg ctacttcgtg caggaatacc
     7081 accgtgcctt tccattcctt cacccgcatg gtgtgcggct tgatacgcgg cagccgttcg
     7141 gcgagttctg caagttcgtg gaagtgtgtg gcaaaaatgg tgcggcagcg gttcgctgaa
     7201 tgcagcgatt ccaggactgc ccaggcgatc gccagcccat ccagcgttgc ggtgccgcgt
     7261 ccgatctcgt ccaccaccac cagcgagcgc ggaccggcct gatgcaggat ggcggcggtt
     7321 tcggtcattt ccaccatgaa ggtggagcgc ccgcgcgcca gatcgtctgc cgcgccgacg
     7381 cgcgagaaca ggcgatccac cacaccgatg gtggcacttt cagccggcac gggcagaccg
     7441 gactgcgcca gcaccaccat cagcgcgttc tgtcgcagga atgtggattt gccggccatg
     7501 ttgggcccgg tcagcaacag cacgcgctgt tccggcgaca ggtcgcaatc gttcgggacg
     7561 aactgcgcgt gccctgccag agcagcttcc accaccgcgt ggcggccggc cttgaccagg
     7621 aatgcgtcat caccggtgac caacgggcgg caccaggtgc ccggttcggc gagcttcgcc
     7681 gccgattgca gcgcatccat gagcgccagc gcatcggcac aggcggcgag cgcatcggct
     7741 tgagagagcg tggattggac gagctgagcg aacacggcgc gttcccgaac ggcagcgcgt
     7801 tcgccagctt ccgaaatgcg ccggtcgaga tcggaaagct ctggcgtggt gaaccgggcg
     7861 ccgttggcca tgccctggcg caacgtgagc tccggatagt cgcggagttt ctcgacggcg
     7921 gccgccggcg cttcaatgac gtaaccgagc tgcgcgtggt gtttgatttt caggctggcg
     7981 acgccgtagc gctgcgcgta gtccaactgc agggtcgcca gcacgcgccg gctgtcatcg
     8041 cgcaaggcgc gctcggcatc cagttcagga tcaaatcccg ggcggatggc gttgccatcg
     8101 tccagccggt gcggtgcagg ttcggcaagg gcatcggcaa gagtttgttc cagcgatgga
     8161 tcaaggcata gcgccccaat tgctgatccc aacagcgatg ggaggctccg ggcaccgttg
     8221 gccgtgacgc aggagagcgc cccgccggca gcacgcagcc catcgcgcat cgccgccaaa
     8281 tccctcggcg tgccccgccc aaccgagagg cgggccagcg cccgggcgat atccggtgca
     8341 gttcgcaacg ccgtgcgcaa tccgtctgcc gcggttcgct cggcgagcag ccatccccac
     8401 gcgtcctgcc gtgccgccac tgccatcgga tcggtcagcg gtgcggccag ccagttcgcc
     8461 agcaaccgcg ccccggccgg cgtcagcgtc cgctggacgg cgccgaggag cgaatgcatc
     8521 ccgccgccgt cgcgcgcacg gtggatctcc aggctggtgc gggttgcggc atccatcgcc
     8581 aataaaccgg cgctcccttg tggcatcgga tgcgccagtc ggggcagtgt gccggcctgg
     8641 gtcgcgcgca cgtaatccac ggccatcagc gccgcgatcg cctcggcgtc tgagaagctg
     8701 ccgaatgcgt cgaggctggc gacgccgaac acctccgcga gccgccgtcg cgcgaccaat
     8761 ggcggcgacg gcgccacatc cggcgcccgt ttttccgccc agtcccccag tgccaacccg
     8821 tccggcgcca ggacttcgac cggctccagg cggccaagca gaccgggcag atccttcgcc
     8881 tgcagcgcca tcgtttcgaa aagcccggtc gagacgtcca gccaggccgc ccctacttca
     8941 tcgcgcgtca acgccaggcc aagcagcaga ttggcgcggc ctgcctccag cagcgcttcc
     9001 tcggtgatcg tccctggcgt caccagccgc accacctcgc gcttgatggg tgctttgccg
     9061 gtgcgcgact tcgggtcctc catctgctcg gtgacggcga cgcggaagcc gcggcggatc
     9121 agccgcgcca ggtatgcctc ggccgcgtgc tgcggcacgc cgcacatcgg cacgggcgtg
     9181 ccattgtgct cgccgcgctg ggtcagcgcg atgtcgagag ccgccgccgc cgcctcggcg
     9241 tcggagaaga acaactcgta gaaatctccc atgcggaaga acacgagtgc gtccggatgc
     9301 gcctccttgg cggcaaacca ttgcgccatg gcgggcgttg cgccggcggc attcggcgtg
     9361 ctgcggaagg atgcggccat cggtttggtt tagcgcgcgg gcgccggatt ccgaaaccca
     9421 accgttggcg gcgctcgtgc tgggagctta aattgccgg
//
LOCUS       DATGEM010000620         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_155831, whole genome shotgun sequence.
ACCESSION   DATGEM010000620 DATGEM010000000
VERSION     DATGEM010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155831"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VKQ27_19175"
     CDS             complement(<1..671)
                     /locus_tag="VKQ27_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91114.1"
                     /translation="MKRSTERILTTHPGRLPNPDNYAEIMAARRSGDQQRFDELTTVA
                     IQDMVRRQKEIGIDIMSDGEFWKVRDQRWYDDRCTGVLVRPLGPGETGFNQVSARSEG
                     RMPEFKAFYEIYDQLGNTPMPGGRPNPPSDMYQVITGPIQVTTAEAVRHEVQRTKDAI
                     LAVGESLENFVFPVISAGWLGHSLHDNYYRDRDKYNYAMAEILRLDYRAVIDAGFALQ
                     IDDPAL"
     gene            915..1520
                     /locus_tag="VKQ27_19180"
     CDS             915..1520
                     /locus_tag="VKQ27_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HLY91115.1"
                     /translation="MTKNRLEAFSDGVIAILITIMVLELRAPAGSDLAALVSALPELA
                     MYLLSFVFLGIYWNNHHHLLHATTAVSGAVLWANLHLLFWLSLVPFATRWMGESGLAP
                     LPTATYGGVLLLSAIAYTILVRAILACQPPDSVLAVAIGDDFKGRLSLGLYAAAVPLT
                     FLNPWIAVAIYTVVALIWLVPDRRIEARLGAAAPPHRARGN"
     gene            complement(1679..2896)
                     /locus_tag="VKQ27_19185"
     CDS             complement(1679..2896)
                     /locus_tag="VKQ27_19185"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY91116.1"
                     /translation="MAPAAAPATTFKNDNQYTGEAASAPYLERIDVFQRWRDKEGVPL
                     VGGVYHRNLKTLEVGPWPRKGVLGAHCYLDGDDGTDEHVVEIPPGKSTIPEHHMYDEA
                     VYVLSGRGACTVSQFDGTKTSFEFGEGAFFAVPLNATVQYHNGSGLQPCRFFSVTNLP
                     QIMRQYLGNEDFIWNCTYDFKDRFAGQDEYFDAKGTLWRGRIWETNFVPDVKGMRLWE
                     WKERGGGGTNAFFILAGGAINSHVSRFQSGTYKKGHWHGPTAHLYVTHGEGYVLMRRE
                     GEDFIKCDWQEGSMYISGAGPGNWLHQHFNVGSIPAEYLVLGVHPARRYAMSRWNAND
                     PTDRNTADLSVKKGGIQVEYEDEERIVHEIFEAELRKRGIQCRMKGYIPWCTGVEGPS
                     KKGEWGDEKVAPV"
     gene            3030..>3850
                     /locus_tag="VKQ27_19190"
     CDS             3030..>3850
                     /locus_tag="VKQ27_19190"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY91117.1"
                     /translation="MRLHVDAYRDWLAAQRLPVLTGHFVADLNTVELEPWELTGGRAA
                     FIYLEGSEGTSGSYVCEIAPGASLKPQRHMYEEIIYILQGRGASTVWGSNGKRHHFEW
                     QTGSLFAVPLNARYQLHNASGSGPVRFVAVTTAPLLMNYFHNPKFIFDNPFCFDDRFG
                     DEDDYFSRDGRMIDTRQIETNFVADVRSLELQAWVERGGGGTNMSFQLSGNTFGAHVS
                     EIAPGNYKKAHRHGGGAHILILSGNGYSLMWPDDGEKIRVDWKPGAFFSPPDIWW"
BASE COUNT          650 a         1212 c         1193 g          795 t
ORIGIN      
        1 agggccgggt cgtcgatctg gagggcgaag cccgcgtcga taacggcgcg atagtcgagg
       61 cgcaaaatct ccgccatcgc atagttgtat ttgtcgcggt ctcgatagta gttatcgtgc
      121 agcgagtggc cgagccaccc agcgctgatg accggaaaga cgaagttctc aagactttca
      181 ccgaccgcga ggatggcgtc tttcgtgcgc tgcacctcgt ggcggaccgc ttccgcggtg
      241 gtgacctgga tgggcccggt aatgacctgg tacatgtccg agggcgggtt tgggcgaccg
      301 ccgggcatgg gcgtgttgcc gagctgatcg tagatctcat agaacgcctt gaactcgggc
      361 atccgacctt cactgcgcgc actgacctga ttgaatccgg tctcgcccgg gccaagcgga
      421 cggaccagga ctcccgtaca acgatcgtca taccagcgct gatcgcggac cttccagaac
      481 tctccgtcgc tcatgatgtc gatgccaatt tctttctggc gtcgaaccat gtcctgtatc
      541 gcgaccgtgg tcagttcgtc aaatcgctgc tggtcgccac ttcgccgcgc ggccatgatc
      601 tcggcgtaat tgtcaggatt gggcaggcgg ccgggatggg tggtcaggat acgctcagtg
      661 ctgcgtttca aggcgatctc ctcttattgt tgcagctcac tgatgagctg aatgtggtct
      721 gtcattgatc cagagtaacc accccgagtg gactcgtgtc gtaccctgta gcatgccggc
      781 gccaggtcgg cctcgaacaa cgggcccggt cttggcgtgt gttaattcgg acagcgcttg
      841 acgatatatc gccagagcgg tgacgacgtg gcgaacggga acgtgacgca tcgaatcggc
      901 gcgaaccagg tggaatgacc aagaatcggc tcgaagcatt cagcgacgga gtgatcgcca
      961 tcctgatcac catcatggtg ctcgagctgc gcgcgccggc cggctcagat ctcgcggctc
     1021 tggtcagcgc cctgccggag cttgcgatgt atctgcttag tttcgtgttt ctcggcatct
     1081 actggaacaa tcatcaccat ctgctccacg cgacgacggc ggtcagcggc gcagtgttat
     1141 gggcaaacct gcacctcttg ttctggctgt cgctggtccc atttgccacc cgctggatgg
     1201 gcgaaagcgg tctggcgccg ttgcccacgg cgacctacgg cggcgtgctg ttgctgtcgg
     1261 caattgccta cacgatcctg gtccgcgcca tcctggcctg tcagccgcca gactcagtcc
     1321 tggccgtggc aattggcgac gatttcaaag gcagactctc gctggggttg tatgccgccg
     1381 cggttccgct gacgttcctc aatccgtgga tcgctgtcgc gatttacacc gttgtcgcgt
     1441 tgatctggct ggtccccgat cggcgcatcg aggcgcgtct tggcgcagca gcaccgccgc
     1501 atcgcgcgcg cggcaattga cttagttggt tcgtcccgta agtttcgatt cctatgcgta
     1561 cccctcaccc caaccctctc cccgcgcggg gagagggttg gggtgagcgt agcgagcggg
     1621 tgaggggtgg cgaccggccc cggaattacg agacgctgta cttagctatg gccagggttt
     1681 agaccggagc gactttctca tcgccccact cacccttctt gctcgggccc tcgacgccgg
     1741 tgcaccacgg gatgtagccc ttcatccgac attggatccc gcgcttgcgc agctccgcct
     1801 cgaagatctc gtggacgatg cgctcctcgt cctcgtactc cacctggata ccgcctttct
     1861 tcacgctcag gtccgcggtg ttgcggtccg tcggatcgtt cgcgttccag cggctcatgg
     1921 catagcggcg ggccggatgg acgcccagca ccaggtactc ggcgggtatg ctgccgacat
     1981 tgaagtgctg gtgcagccag ttgcccgggc cagcgcccga gatgtacatg ctgccctctt
     2041 gccagtcgca cttgatgaag tcctccccct cgcgccgcat gaggacgtag ccctcgccat
     2101 gggtgacgta cagatgagcg gtcggcccgt gccagtggcc tttcttgtac gtacctgact
     2161 ggaagcgcga aacgtgcgag ttgatggcgc cgccggcgag aatgaagaac gcgttggttc
     2221 cgccgccgcc gcgctctttc cactcccaca accgcatgcc tttcacatct gggacgaagt
     2281 tcgtctccca gattcgaccg cgccagagcg tgcccttcgc gtcgaagtac tcgtcctggc
     2341 cggcgaaacg gtccttgaag tcgtatgtgc agttccagat gaagtcctcg ttgccgaggt
     2401 actgccgcat gatttgcggc aggttcgtca ccgagaagaa gcggcacggc tggaggccgc
     2461 tgccgttgtg gtactgcacc gtggcgttga gcgggacggc gaagaaggcg ccctcgccga
     2521 attcgaagct ggtcttcgta ccgtcaaact gggacacggt gcatgcgccc ctgcccgaca
     2581 ggacgtagac cgcctcgtca tacatgtggt gctcggggat ggtcgacttg ccaggcggga
     2641 tttccaccac gtgctcgtcg gtgccgtcgt ctccgtcgag gtagcagtgc gcgcccagca
     2701 cgcccttgcg cggccacggg ccaacctcga gggttttcag attgcggtgg tagacgccgc
     2761 cgaccagcgg cacgccttcc ttgtcccgcc agcgctggaa gacgtcgatt ctctccaggt
     2821 acggcgctga cgccgcctcg cccgtgtatt ggttgtcgtt cttaaaagtc gtggccggcg
     2881 cggcggcggg tgccacctcc gttagcccta ctggccggtc tggtgcttgc gtcatcgagc
     2941 ctcccgtcag cgctcccgat agtactcttc tcaagcatag ccgcaattta tgaattcgag
     3001 gggaatgcat ggcccagact gtccccgacg tgcgcctgca cgtcgacgcc tatcgcgatt
     3061 ggctcgccgc gcagcgcctg cccgtgctga ctggccattt cgtggccgat ctcaacaccg
     3121 tcgagctcga gccgtgggag ctgacgggcg ggcgggccgc cttcatttac ctagaaggtt
     3181 cggagggaac ctctggctcg tacgtatgtg agatcgcgcc cggcgcgagc ctgaaacccc
     3241 agcggcacat gtatgaggag attatctaca tcctgcaggg gcgcggcgct tcgaccgtct
     3301 ggggcagcaa tggcaaacga caccacttcg agtggcagac cggcagcctg ttcgccgtcc
     3361 cgctcaacgc ccggtaccag ctgcacaatg ccagcggcag cggcccggtc cgcttcgtcg
     3421 ccgtcaccac cgcgccactg ctgatgaact acttccacaa cccgaagttc atcttcgaca
     3481 acccattctg cttcgatgac cgcttcggtg atgaggatga ctacttttcg cgcgacgggc
     3541 gcatgatcga tacgcgccag atcgaaacga acttcgtggc tgatgtgcgc agcctggagt
     3601 tgcaggcgtg ggtcgagcgc ggcggcggcg gcacgaatat gagtttccag ctctccggta
     3661 acacgttcgg tgcgcacgtc tcggagatag cgcccggcaa ctacaagaag gctcaccgcc
     3721 acggcggcgg cgcgcacatc ctgatcctgt ccggcaacgg ctacagcctg atgtggccgg
     3781 acgacgggga gaaaatccgc gtagattgga agccaggtgc cttcttctcg ccgccggaca
     3841 tctggtggca
//
LOCUS       DATGEM010000621         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1639786, whole genome shotgun sequence.
ACCESSION   DATGEM010000621 DATGEM010000000
VERSION     DATGEM010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639786"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VKQ27_19195"
     CDS             <1..407
                     /locus_tag="VKQ27_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91118.1"
                     /translation="VAMRIVGLALLVALPLAPSFGVAASLYMLRTMFNRGTTGARSAL
                     NVSIVRSHRRGFAASMGNVSMQVPRAVSPLLTGILFAANDLVLPFFIGAAFQAAYLVI
                     YYSSFRWVDDPTPQHGRDGPPVTVAASVNRQP"
     gene            479..1651
                     /locus_tag="VKQ27_19200"
     CDS             479..1651
                     /locus_tag="VKQ27_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HLY91119.1"
                     /translation="MTFPAWLPILLGFLTAVGPATTDMYLPAFPALEHSFGAPPGSAQ
                     LTLAAWFAGLSVGQLTQGTLSDRFGRKRPLIVATSIYTLACLGCALAPSIQWLAAFRA
                     LAAVGASAGMVIPRAMVRDLAEGHAAAVLLSRLALVMGAAPILAPTLGSAVLAFADWR
                     GIFWILTTYGAICLTLVVLLLPDTLPQDRRIRLHLGEYLARCRSILTERSFLTHASMG
                     AFATFAFFAYLGGSSPVFIQGFGLSPSGFAMIFGANSFGLICCAQLNPHLLRRIGHVV
                     LLRWVARVHFCAVGTLTAIAFAGLHVLPLVIAPLIVAISCMGMLNPNTIVGALARHQN
                     HAGTASSVMGTGQYLLGAISGLLVGIFTDGTPRGMAALMLVGSLGMAVADKFRPHT"
     gene            1682..2428
                     /locus_tag="VKQ27_19205"
     CDS             1682..2428
                     /locus_tag="VKQ27_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HLY91120.1"
                     /translation="MPPIVGIPACAIHVVKDQLRHDTPARYCAAVLGGSGAVPIMIPP
                     VGEAALAVLGRIDGLLLPGSPSNVHPSHYDGGESQTPDRHDLERDATTLRLIPEAIKR
                     GIPVLAICRGIQELNVALGGTLHQQVHVVARRMDHRPGPGALEEKYAPTHLVSLSGQL
                     ARIVGSAKILVNSLHGQAIDTPAPGLAVEAVAPDGTIEAVRVEKASGFAFGVQWHPEW
                     GYADDSASLAIFRAFGDACRAYQSGLKKAA"
BASE COUNT          359 a          884 c          751 g          481 t
ORIGIN      
        1 tggtcgccat gcgcatcgtc gggctggcct tgctggtcgc gttgccgctc gcgccgagtt
       61 tcggtgtcgc cgcgtcgctc tacatgctgc gcacgatgtt caatcggggc accaccggcg
      121 ctcgtagtgc actgaacgtc agcatagtca ggtcacatag gcgcggcttc gcggcgagca
      181 tgggaaatgt ctcgatgcag gtaccgcgag cggtaagccc gctgctgacg ggcattctgt
      241 tcgccgccaa cgatctcgtc ctgccgttct ttatcggcgc ggcgttccag gcagcctatc
      301 tcgtgatata ttattcgagc ttccgttggg tcgatgatcc gacaccgcag catggccgcg
      361 acggaccgcc ggtgaccgtc gcagcgtcgg tcaatcgcca accctaagac ctgacccggc
      421 gcagtggcga acgtcggcac ccctggccat ctgaaccgcc ccctgcaaca accgcactat
      481 gacgttcccc gcctggctac ccatcctgct cggcttcctc acggcggtcg ggccggcgac
      541 gacggatatg tacctcccgg ccttccccgc gctggagcac tcgttcggtg cgcctcctgg
      601 ctccgcgcag cttaccctcg ccgcctggtt cgccggcctg tccgttggcc agttgaccca
      661 gggtaccctc tccgaccggt tcggccgcaa gcggccgttg atcgtggcaa catctatata
      721 tacacttgcc tgcctcggct gcgccctcgc cccctctatc cagtggcttg ccgcattccg
      781 cgcgttggcc gcggtcggcg cctcggccgg gatggtcatc ccgcgcgcca tggtgcgcga
      841 cctggccgaa ggtcacgccg ccgccgtcct gctctcgcgg cttgcgctgg tgatgggcgc
      901 cgcgccgatc ctcgcgccga cgctcggcag tgccgtgctg gcgttcgccg actggcgtgg
      961 gatcttctgg atccttacca cctacggcgc gatctgcctg actttggttg tcctgctgct
     1021 ccccgacacg ctcccacagg accgtcggat caggctgcat ctcggcgagt atcttgcacg
     1081 ctgccgcagc attctcactg agcgcagctt cctcacccat gcctcgatgg gcgcattcgc
     1141 caccttcgca ttcttcgcct atctcggcgg ttcgtcgccg gtgttcatcc agggcttcgg
     1201 cctgtcgcca tcaggctttg cgatgatctt cggcgccaac tccttcggac tgatctgctg
     1261 cgcgcagctc aacccacatc tgttgcgccg catcggccac gtcgtcctgc tgcgctgggt
     1321 cgcgcgcgtg cacttctgcg ccgtgggtac gctcaccgcc atcgccttcg ctggattgca
     1381 cgtcctcccg ctggtgatcg cgcctctgat cgtggcgata agctgcatgg gcatgctgaa
     1441 tcccaacacc atcgtcggcg cgctcgcgcg gcaccagaac cacgccggca ccgcgtcctc
     1501 ggtgatgggt acgggccagt atctgctggg cgcgatcagc ggtctgctgg tcgggatctt
     1561 caccgatggc acgccgcgcg gcatggcagc cctcatgctg gtggggtcgc tcgggatggc
     1621 ggttgcagat aagttccggc cgcacacata gtgcccgaat cgccaaggag gtccgcctcg
     1681 catgccccca atcgtcggca tccccgcctg cgccatccat gtggtgaagg accagcttcg
     1741 ccacgatacg cccgcccgtt actgcgcggc ggtgctcggc ggctccggcg cagtgccgat
     1801 catgatcccg ccagtcggtg aggccgctct cgcggtgctc ggccggatcg atggattgct
     1861 gctgcccggc agcccgagca acgtccatcc ctcccactac gatggcggcg aaagtcagac
     1921 acccgatcgc cacgacctcg agcgggatgc caccacgctc cggctgatcc ccgaggcgat
     1981 caagcgcggc attccggtgc ttgcaatctg ccgcggcatc caggaactga acgtggcgct
     2041 cggcggcacc ctgcaccagc aggtgcacgt ggttgctcga cgtatggatc atcgccccgg
     2101 tccaggcgcg ctcgaggaga aatacgcacc cacccatctg gtatcgctgt ccggccagct
     2161 tgcgcgcatc gtcggcagcg ccaagatcct ggtgaactct ctgcacggtc aggcgatcga
     2221 cacgcccgcg cccggtctcg cggtggaagc cgtggccccc gatggcacga tcgaagcagt
     2281 gcgcgtcgaa aaggcctcgg gctttgcatt cggcgtccag tggcaccccg aatggggcta
     2341 cgccgacgat agcgccagcc ttgccatctt ccgcgcgttc ggcgacgcat gccgtgccta
     2401 ccaatccggc ctgaaaaagg cagcgtagct atcaatttgt cattgcgagg aggcgtagcc
     2461 gacgaagcaa tctcg
//
LOCUS       DATGEM010000622         6054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1412220, whole genome shotgun sequence.
ACCESSION   DATGEM010000622 DATGEM010000000
VERSION     DATGEM010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412220"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VKQ27_19210"
     CDS             complement(<1..290)
                     /locus_tag="VKQ27_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91121.1"
                     /translation="MIGVALKMLAGDRGKFLAIVIGIAFSSFLTAYAACYFAGFMSRS
                     YALVAEHAGVDIWVMDAKTVSAEQFANMPESALYRVRSVAGVAAAERMAIGQA"
     gene            complement(287..1213)
                     /locus_tag="VKQ27_19215"
     CDS             complement(287..1213)
                     /locus_tag="VKQ27_19215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLY91122.1"
                     /translation="MTNLMKILVAAAAAGMVVTAGAVVHDRNAGGAAISTMDAKSADF
                     GGDGQIAGSGLVEAGSGTIAVASPVAGVVASVAVQWGEQVHRGDLLFRIDDRDIADTR
                     PVAAAELEKARLAIPAASQAFMAAQRLLADQLTTMQDVSTRKADLAAAHAAVTVAEAQ
                     LRRIDAEIARRTVRAPTSGRILKINLRPGEYADPASSAGPPLLMGSDQRLNVRAEFDE
                     YDVWRLRDGTAAIGTVPGNPAIRIPLRFERIEPYVLPKTNLSGAATERTDTRVLQVIY
                     SFDPKSLPVYIGQRLDVEVATTGRSARVAARP"
     gene            complement(1210..1935)
                     /locus_tag="VKQ27_19220"
     CDS             complement(1210..1935)
                     /locus_tag="VKQ27_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91123.1"
                     /translation="MMPPEGPQRGAANPAISCRGLTKRYGTGDALVTALRGVDLDVER
                     GEVLMLMGPSGCGKTTLLSILATLLAADEGTCVLLGEPISTGADRRAHLRASTLGFVF
                     QSFNLLPALTAIENVAIPLTLAGMGREALSRAHEALVSVGLNSKIGTLPRQLSGGQQQ
                     RVAIARAIVHRPQILLCDEPTSALDHDTGQSVMETLCAAARAQNAAVVIVTHDARITH
                     FADRIAYMEDGRLFEGDGAVARP"
     gene            complement(1970..2506)
                     /locus_tag="VKQ27_19225"
     CDS             complement(1970..2506)
                     /locus_tag="VKQ27_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HLY91124.1"
                     /translation="MEMQWLLIAYKVPPEPAAGRVAIWRRLKGMGAVYLQNGVCLLPK
                     TDDHIRRLKMLENDATEMGGEAVILETIGFDASQEQKVLERFRADRDDQYREFIGKCD
                     AFEAEIAKEFRIEKFTFAELEEEDAELRKLQGWLEKIRKLDFYDAALGGTAGERLRAC
                     EMLLDQYAQRVFEVQGEK"
     gene            complement(2644..3633)
                     /locus_tag="VKQ27_19230"
     CDS             complement(2644..3633)
                     /locus_tag="VKQ27_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLY91125.1"
                     /translation="MIRAVLVAIAALIATSCGAAPTTLKAADPRSPLRLEHVIVLPGV
                     KGRIDHLALDLEHRRLFVAEYRNGTVDEIDLANGRLEGRILGLHEPQGVAYLGRDGEI
                     VVACGDGSVHFYSATDRHEVARIDLHDDADNVRIDERNGYVIVGYGKGGLATIDPETH
                     RVIGRMELPGHPEGFRLVGARVLINVPDRGAILAGDVDKGKVESTWSTGSHRLNFPLS
                     VAPTGAWFAIAYRLPAALAQFDSGTGKQLEIRSACGDADDLFIDGKRIFLVCGAGHVD
                     VMPTGTGGSVVRVATGSGARTGLFSAELHTLFVAVPSRGQPAAIWVLRTEPAH"
     gene            3886..4131
                     /locus_tag="VKQ27_19235"
     CDS             3886..4131
                     /locus_tag="VKQ27_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91126.1"
                     /translation="MTGSSADVEDATLDSAQFDWKDQATRLPRLTHWLVPYLQKGALK
                     RFRRPPGRHLYLDDPAWFDHVAAQADVSLGELAVVWL"
     gene            4391..>6054
                     /locus_tag="VKQ27_19240"
     CDS             4391..>6054
                     /locus_tag="VKQ27_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY91127.1"
                     /translation="MKRVAIGIAACFAATSVQAKERKVDFNVPPGPLNAAIPALGQQA
                     GITIGSGDPLLGSTRTRGVSGHLTIPAALTRMLAGTGFTFIPISANAVRIERSTPVVR
                     GDRPRPMWKAQETAALSLGDITVTASKQSTSLSRFAGTVRFVDVGDPANARSGARGTE
                     LLLDRLPMLASTNLGPGRNKLYIRGIADSSFNGPSQSIVGQYLGDIRLTFNAPDPDLQ
                     LYDMKRVEVLEGPQGTLYGSGALGGILRLIPNEPDPRAFSGALSTGGLVTQHGGTGGD
                     IAATGNLPLVADRLALRVVGYGSRSPGYINDIGLGRSNVNRTTIYGGRATTRWDAGNG
                     WAVDLGGLIQFIHGRDGQYAMAGLPPLTRRSYLAQPFHNDYKLGHLVIRKRWGDVEFV
                     SATGVVEHDVSADFDATDPRQSPVRQIFLERTAVRMISNETHLSRSGPNGDGWVAGMS
                     IVHDTDRLSRHFGAVSNPPPIAGVRNETTEAALFGQFGYAITDTLVATVGGRLTFADT
                     TGLSVDTRSDHMEPRRRELRLTPTAGIAWRGLTGTTLFVRYQQGYRA"
BASE COUNT         1147 a         1980 c         1834 g         1093 t
ORIGIN      
        1 gcttggccga tcgccatccg ctcggccgct gcgacgccgg caacggatcg cacccggtac
       61 agggccgatt ccggcatatt ggcgaactgc tcggccgaca cggtcttggc atccataacc
      121 cagatatcga cgccggcgtg ctcggccacg agcgcgtagc tccggctcat gaagcccgcg
      181 aaatagcagg cggcataggc cgtcagaaag gacgagaagg cgatgccgat cacgatcgcc
      241 aggaacttgc cacgatcccc ggccagcatt ttgagcgcga cgccgatcat ggccgcgctg
      301 cgacgcgcgc gctcctgccg gtcgtcgcca cctccacgtc gaggcgctgg ccgatataga
      361 ccggaaggct cttcggatcg aagctgtaga tcacctggag gacgcgtgtg tcggtgcgct
      421 ccgtcgcggc gccggagaga ttggttttcg gtagcacata gggttcgatc cgctcgaaac
      481 gcagcggaat cctgatcgcc ggattacccg ggactgtacc tatcgcagcc gttccatcgc
      541 gcaggcgcca gacatcatat tcgtcgaatt cggctcggac attcagcctt tgatcgctgc
      601 ccatgagaag cggtggcccc gccgagctcg ccggatcggc atattcaccg ggtcgcagat
      661 taatcttgag gatgcggccg ctggtcggcg cacgtacggt gcgccgcgcg atctcggcat
      721 cgatccgccg gagctgcgct tccgccacgg tcacagctgc gtgcgcagcg gcaagatccg
      781 ccttgcgcgt cgacacatcc tgcatggttg taagctgatc ggccagaagg cgctgggcgg
      841 ccatgaatgc ctggcttgct gccggtatcg caagccgggc cttttccagc tccgccgcag
      901 cgacaggcct ggtgtcagcg atgtcgcgat cgtcgatccg aaacaacaga tcaccgcgat
      961 ggacctgctc cccccattgc accgcgaccg atgcgacgac gcccgcaacc ggcgatgcga
     1021 ccgcgatcgt gccgcttccc gcttcgacaa gaccacttcc agcgatctgc ccgtcgccgc
     1081 caaaatcggc cgacttggca tccatggttg agatcgctgc cccgccggcg tttcggtcat
     1141 ggacaaccgc gcctgcggtg acgaccatcc cggccgcagc tgccgccacc aatatcttca
     1201 tgagatttgt catggacgag ccactgctcc gtcgccttcg aagagccgac cgtcttccat
     1261 atacgcgatc cgatcggcga agtgggtgat gcgggcgtca tgcgtcacga tcacgaccgc
     1321 ggcattttgg gcacgcgcgg cggcgcacag cgtctccatc acggattgac cagtgtcatg
     1381 atcgagcgcg ctggtgggct catcgcaaag caatatctga gggcgatgca cgatcgcgcg
     1441 cgcgatcgcg acgcgctgct gctgaccgcc actgagctgg cgcggcaaag tgccaatctt
     1501 gctgttcaga ccaaccgaca ccagcgcttc atgcgccctc gatagggctt cacggcccat
     1561 gcctgccagc gtcaggggta tcgccacatt ctcgattgcc gtcagcgcgg gcaagaggtt
     1621 gaacgactga aagacgaagc cgagcgtaga cgcccgcaag tgcgcgcgtc gatcggcgcc
     1681 cgtgctgatc ggttcaccga gcagcacgca tgtgccctcg tcggccgcca aaagggtggc
     1741 gaggatcgat agcaaggtcg tcttcccgca ccccgaaggc cccatcagca tgagcacctc
     1801 gccccgctcg acatcgagat cgacgccacg caacgccgtc acgagtgcgt ctcctgtgcc
     1861 gtagcgcttg gtgagacccc ggcacgagat agccggattc gccgcaccac gctgcggtcc
     1921 ttcgggaggc atcatcgatt gcgtcccccg tcgtggcttg cctgagacct catttttcgc
     1981 cctgcacctc aaacacacgc tgggcatatt gatcgagcag catttcacag gctcgcagcc
     2041 gctcgccggc tgtgccacca agggcggcat cgtagaagtc gagcttccgg atcttctcga
     2101 gccatccctg gagcttgcgc agttccgcgt cctcctcctc cagctccgcg aaggtgaatt
     2161 tctcgatccg gaactccttg gcgatctcgg cctcgaaggc atcgcatttg ccgatgaatt
     2221 cgcgatactg atcgtcgcga tcggcacgga agcgctcgag aaccttctgc tcttgggatg
     2281 catcgaaacc gatcgtctcg aggatgaccg cctcgccacc catctccgtc gcgtcgtttt
     2341 caagcatctt caagcggcgg atatggtcgt cggttttggg caggaggcag acgccattct
     2401 gcagataaac cgcacccatg cccttgaggc gccgccatat cgccacccgc ccggcggcgg
     2461 gttcgggggg aactttgtac gcgatcagta accactgcat ttccatagct cacagataat
     2521 gtaaccatcg ttacaaaaca agcaaatcgc cgcacggata cctgctcagg gctcgtgctt
     2581 ttcggcaatt cgatgaggaa gcggcgaatg tcagagcgac gcagcaactc gatcgcttaa
     2641 cgatcagtga gccggttcag tcctgagaac ccagatcgcg gccggttggc cgcgcgatgg
     2701 caccgccacg aagagcgtgt gcagttcggc ggagaacagg ccggtcctgg ctccgctccc
     2761 cgtcgcaact cggacaacgc tgccacccgt tccagtcggc atcacgtcga cgtggcctgc
     2821 accacagacg aggaaaatcc gcttgccatc gatgaataga tcatcggcgt caccacacgc
     2881 agatcggatc tcgagttgtt tgcctgtgcc actgtcgaac tgcgccaacg cggccgggag
     2941 gcggtaggca atggcgaacc acgctccggt cggcgcgaca gatagcggaa aattcagccg
     3001 gtgcgatccg gtcgaccagg tcgactcgac cttgcccttg tcaacgtcgc cggccagaat
     3061 ggcgccgcga tccggcacgt tgatgaggac gcgggcaccg accagccgaa agccctcggg
     3121 atgacccggc aactccatgc ggccaatgac ccgatgcgtt tccggatcga ttgttgcgag
     3181 gcctcctttg ccgtagccga cgatgacata accgttgcgc tcgtcgatcc ggacattgtc
     3241 ggcgtcgtca tggaggtcga tgcgcgcgac ttcgtgacga tccgttgcac tatagaaatg
     3301 aacggatcca tcaccgcaag cgacgacgat ttcaccgtcc cttcccaggt aggcgacgcc
     3361 ctgcggttcg tgcaggccga gaatgcggcc ctcgaggcgg ccatttgcga gatcaatctc
     3421 atcgaccgta ccgttccggt attcggcgac gaagagacgg cggtgctcta ggtcgagcgc
     3481 gagatggtcg atgcgcccct tcacgccggg gagtacgatg acgtgttcga gacggagcgg
     3541 cgaccgcgga tcggctgcct tgagcgttgt aggcgctgcg ccgcacgacg tcgcgatcag
     3601 cgcggctatg gcaacgagaa cagcccggat catatctccc ccccaaagtg cgggcagctg
     3661 gcggcgccag ccgatggacc ggccggcaaa tctacgtgca gtcgaccaac atcttgtttg
     3721 cctacgcagt cattcctcac ctttgaaccg ctcccccgta ccgtggctcg cacgccggca
     3781 aaccgacaat ataggccaaa cgcgagctca ctacttatgc ctggtcaatc atgactggcg
     3841 tggcgctcga cacatgacag gcgcgttttc aagagcagat tgcatatgac gggcagcagt
     3901 gcggatgtcg aggatgctac tttggatagt gcgcaattcg actggaagga tcaggcaacc
     3961 cggttgccgc gtcttacgca ttggcttgtg ccgtacctcc agaaaggcgc cctcaaacgc
     4021 tttcgtcgtc cacctggacg gcatctctat cttgatgatc ccgcctggtt cgatcatgtc
     4081 gcggcccaag cagatgtaag ccttggcgag ctagctgttg tctggttgtg agacctcgtt
     4141 ggcataatag aggatgtcat gcctgtcata ttctcaatgc cagtacgatt caccgcgaag
     4201 gcctcaagat gaatgacctt gagaagttgg ccgaggaagt ccaaaaattc gttgccgaag
     4261 atgagagcct tgcatggtcg cgggcggatc tggaggggat aatcgcccct ttcgggcaca
     4321 gggagcgcga cacgggccgg ctccattcag gcagcggcgt cgagaatgcc tggcgcaggc
     4381 agcgcgtatg gtgaaacgcg ttgcaattgg catcgccgca tgttttgcgg ccaccagcgt
     4441 ccaggcgaag gagaggaagg tcgacttcaa cgtcccgccg ggaccgctca atgctgcgat
     4501 cccggcgctt ggccagcaag ccggcatcac gatcggctct ggcgacccgc tgctcggatc
     4561 gacgcgcacc cgcggtgtga gcggccatct gacgatcccc gccgcgctta cccggatgct
     4621 tgcgggcacg ggtttcacgt tcatccccat ttccgctaac gccgtacgca tcgagcgcag
     4681 cacgccagtc gtccgtggtg atcgaccgag gccgatgtgg aaagcgcaag agaccgccgc
     4741 gctctcgctg ggcgacatca cggtcacagc cagcaagcag tccacgtcgc tgagccgttt
     4801 tgcgggcacg gttcgcttcg tggacgtcgg cgaccccgcc aacgcccggt ccggcgcccg
     4861 cggcaccgag ctcctgctcg accgcctgcc gatgctggcg tcgaccaacc tcgggcccgg
     4921 gcgaaataag ctctatatcc ggggtatcgc ggacagcagc ttcaacggtc ccagccagtc
     4981 gatcgttggc cagtatctcg gcgatatccg cctgaccttc aatgcgccag acccggacct
     5041 ccagctctac gacatgaagc gagtggaggt cctcgaaggg ccgcagggca cactgtacgg
     5101 aagcggtgct ctcggaggca ttcttcggct gattccgaac gagcccgatc cgcgcgcctt
     5161 ctccggcgcg ctctcgaccg gcgggctcgt cacgcagcac ggcggcaccg gtggcgatat
     5221 cgccgcgacc ggcaacctgc cgctggtagc ggatcggctc gcgctgcgcg tcgtcggcta
     5281 tggatcgaga agccccggtt atataaacga tatcgggctt ggccgaagca atgtgaaccg
     5341 cacgaccata tatggcggtc gcgccaccac gcgatgggac gccggcaatg gctgggccgt
     5401 cgatctgggc gggctgatac aattcatcca cggcagggat gggcagtacg ccatggcagg
     5461 actaccgccg ctcacccggc gctcttacct cgcccagccc ttccacaacg actacaagct
     5521 cggtcacctc gtcatccgca aacggtgggg agatgtcgag tttgtgtctg cgaccggcgt
     5581 ggttgagcat gacgtcagcg cagacttcga cgccacagac ccgcgccaat cgccagtccg
     5641 gcagatattt ctcgaacgca ccgcggttcg gatgatctcg aacgagaccc acctatcgcg
     5701 cagcggcccg aatggggacg gctgggtcgc cggcatgagc atcgtccacg acaccgaccg
     5761 gctatcgcgc catttcggcg cggtctcgaa cccacccccg atcgccggcg tccgcaatga
     5821 gaccacagaa gccgccctgt tcggccaatt tggctatgcg atcaccgaca cgctggtagc
     5881 aaccgtcggc ggccggctga ccttcgccga cacgaccggg ctctcggtcg acacacgaag
     5941 tgatcatatg gaacccaggc gccgcgagct gcggctgacg ccgacggcgg gcatcgcctg
     6001 gcggggctta accggcacca cgctgttcgt ccgctatcag caggggtatc gggc
//
LOCUS       DATGEM010000623         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_41719, whole genome shotgun sequence.
ACCESSION   DATGEM010000623 DATGEM010000000
VERSION     DATGEM010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41719"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VKQ27_19245"
     CDS             complement(<1..1232)
                     /locus_tag="VKQ27_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91128.1"
                     /translation="MIRGAWLAPFQVRSFRFQYPADLLTSWGSEMENLILGWYILVTT
                     GSVVLLTLFGSLQYFGTLVAPMFGMAGDRFGQRIVMCVMRAFYAVLASVLMSLAFSGV
                     LRPVHVFIIAAIAGLVRPADLAMRAALVAETMPTDRFVSAMGASRTTSDSARTAGSLA
                     GAGLFAALGLGSAYVFITGFYLVGFLLTLGVGGTRVVRPAGALRASLWRDLRDGLEHL
                     WDTPSSLAALWLAFLVNLTAFPMTSGLLPYVARDVYHVDQTGLGSLISSFAFGSLLGS
                     ILISVIGRSIRPARMMVVFALGWYAMLLVFVRTPDPLSGCGVLICAGLSQSLSLVPMS
                     AMLLHGAGAYRGRVMGMRMLAIYGLPLGLLAAGALIERIGFVATATGYCVVGLVLTLL
                     IALRWREALWPLHAPANAR"
     gene            1491..2273
                     /locus_tag="VKQ27_19250"
     CDS             1491..2273
                     /locus_tag="VKQ27_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91129.1"
                     /translation="MAAFILLCASAALGMYVRPRLPERHRTRETTELMQITIGLLVTF
                     AALVLGLLTATVKQRYDDTAQDRQEYALQLTMLDRCLGDYGPEAASIRADIQSYTAAV
                     IASTWPDESPPTGVQYPNTTSMPRIGASPVLGELMSRVGTEITRLTPASAPQNRMLDL
                     CLQRYRDVVQARLGVVEDARTRLIEPFYVVLMFWLMIIFGCFGLIAPRTSLSVIVIVL
                     CAVSLSSVIFVILDLSRPYQGYFAVASATMRTALDNMLNAAH"
     gene            2334..>3134
                     /locus_tag="VKQ27_19255"
     CDS             2334..>3134
                     /locus_tag="VKQ27_19255"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLY91130.1"
                     /translation="MESAHVFILAGGLLGVLSIFAAIASRRLGAPVLLVFLVLGMLAG
                     EDGVGGIVYNDFSSAYLIGSVALAVILFEGGLKTHLSMLRLALWPALALAVIGVAITA
                     VIVGATVMWIASVPFSLAMLIGAAVAPTDAAAVGSMLAKARLALPERVLALLEVESGL
                     NDPMSIFLTVFVIHVIAEPASATWQSGLQLFAQEMFGGGALGLGGGWLLRSLLHRISF
                     EIPTAMVLVLTFGLALFGLAQMLHTSGFMAVYLAGIVIGATEYRGRQEV"
BASE COUNT          564 a         1155 c          881 g          534 t
ORIGIN      
        1 cgcgcattcg ccggcgcatg cagtggccat agcgcctccc gccaccgcag cgcgatcagc
       61 agcgtcaaca ccaaccccac cacgcagtac cccgtcgctg ttgcgacaaa cccgatccgt
      121 tcaatcagcg cccccgccgc cagcagcccc agtggcagcc cgtagatcgc cagcatccgc
      181 atccccatca cccggccgcg atacgccccc gccccgtgca gcagcatggc cgacatcggc
      241 accaggctca ggctctgcga cagcccggcg caaatcagca cgccgcaccc gctcagcgga
      301 tccggcgtgc gcacgaacac cagcagcatg gcgtaccatc ccaacgcgaa cacgaccatc
      361 atgcgtgccg gcctgatcga ccgcccgatc acgctgatca ggatcgaccc aagcagtgag
      421 ccgaacgcga agctggagat cagcgacccc agcccagtct ggtccacgtg gtagacatcg
      481 cgcgccacgt aaggcagcag cccgcttgtc atcggaaatg cggtcaggtt caccaggaac
      541 gcgagccata gcgccgccag cgaggacggc gtgtcccaca gatgctccag cccatcccgc
      601 aggtcccgcc acagcgaagc ccgcagtgcg cccgctggac gcaccacacg cgtcccgccc
      661 acaccgagcg tcaacaggaa acccaccaga tagaagccgg tgatgaatac ataagctgaa
      721 cccagcccga gtgcggcgaa cagtccggct cccgccagcg atcccgccgt ccgcgccgaa
      781 tcggacgttg tccgggacgc ccccatcgcg ctgacaaacc ggtccgtcgg catcgtctcc
      841 gccaccagcg ccgcgcgcat cgcgagatcc gccggccgca ccagccccgc gatcgccgcg
      901 atgatgaaca catgcaccgg ccgcagcacg ccactgaacg caaggctcat cagcacgctc
      961 gcaagcaccg catagaacgc ccgcatcacg cacatgacga tgcgctgtcc gaaccggtcg
     1021 cctgccatgc cgaacatcgg cgccaccagc gtcccgaaat actgcaagga gccgaacagc
     1081 gtcagcagca cgacagagcc tgtcgtcacc aggatgtacc accccaggat caggttctcc
     1141 atctcgctgc cccaggaggt cagcagatcg gctggatact ggaagcgaaa gctgcgcacc
     1201 tggaacggtg ccagccatgc gccgcggatc atcgcctgtc gggcctttcg tgtttcctgg
     1261 acggcctgac aggcctgtta cccgcgagcc tatcgcggca tcgcaccgat ccgccagggc
     1321 ctggacgcgc cgcaccacac gacagcaacc ggtcgcccgc gccggggata cgacccgtat
     1381 agttgtgatt cctggttgcc cgctccttcc ccgcccgcca atctgcggcc gcttcgaaca
     1441 tcaggaattg acgaagagga acgcaacggt tgcgcgaggt cggttcagta atggcggcct
     1501 tcattctgct ttgcgccagc gcggcgctgg gcatgtatgt ccgtccacgc ctgcccgaac
     1561 gtcaccgcac ccgcgaaacc accgagctga tgcagatcac catcggcctg ctggtcacct
     1621 tcgccgcatt ggtgctcggc ctgctcaccg cgacggtcaa gcagcgctac gacgataccg
     1681 ctcaggaccg ccaggaatac gcgctgcaac tcaccatgct ggatcgctgc ctcggcgact
     1741 atggcccgga agccgcatcc atccgagccg acatccagag ctacaccgcc gcggtcattg
     1801 ccagcacctg gccggacgag tcgccaccca ccggcgttca atacccaaac accaccagca
     1861 tgccgcgtat cggcgcgtcc ccggtgcttg gcgaactgat gagccgtgtc ggcactgaaa
     1921 tcactcggct cacgccggca agtgcacctc agaatcgcat gctcgatctt tgcctgcagc
     1981 gatatcgcga cgtcgtgcag gcccgcctcg gcgtcgtcga ggatgccagg acacggttga
     2041 tcgagccatt ctacgtcgtc ctgatgttct ggctgatgat catcttcggc tgtttcggac
     2101 tgatcgcacc gcgcacgagc ctgtccgtga tcgtgatcgt cctgtgcgca gtctcgctca
     2161 gcagcgtgat cttcgtcatc ctggacctca gccgtccata tcagggctac ttcgccgtcg
     2221 ccagcgccac catgcggacg gcactggaca acatgctgaa tgccgcacac tgatccaatg
     2281 ccgatatgac aatatccaac ggacctcagc ttctcgagat gggccgcgaa cacatggaat
     2341 ccgcccatgt gttcatcctc gccggtggcc tgctcggcgt gctcagcatc ttcgctgcga
     2401 tcgcctcgcg ccgtctcggc gctcccgtcc tgctggtctt cctggtactc ggcatgctcg
     2461 ccggcgagga cggcgtgggc ggtatcgtct ataatgactt ttcctctgcc tacctgatcg
     2521 gcagtgtggc cctcgccgtc atcctgttcg agggtggcct caagacgcac ctttccatgc
     2581 ttcgcctcgc gctgtggccc gcattggccc tcgcagtgat cggcgttgcc atcaccgccg
     2641 tcatcgtcgg cgccacagtc atgtggatcg cctcggtacc cttcagcctc gccatgctga
     2701 tcggcgccgc agtcgctccc actgatgccg ccgctgtcgg ttccatgctt gccaaggcgc
     2761 gcctcgccct gcccgagcgc gtcctcgcgc tgctggaagt cgagtcaggt ctcaacgacc
     2821 ctatgtcgat cttccttacc gtgttcgtca tccatgtcat cgccgaaccc gcgagcgcca
     2881 cctggcagag cggtctgcag ctgttcgcac aggagatgtt cggcggcgga gcgctggggc
     2941 tcggcggcgg ctggctgctg cgcagcctgt tgcatcgcat ctcgttcgag atcccgaccg
     3001 ccatggtgct ggtgctcacc ttcggcctgg cgttgttcgg cctggcgcaa atgcttcaca
     3061 ccagtggctt catggctgtc tatctcgccg gcatcgtcat cggcgccacc gagtaccgtg
     3121 gccgccagga ggtt
//
LOCUS       DATGEM010000624         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1267699, whole genome shotgun sequence.
ACCESSION   DATGEM010000624 DATGEM010000000
VERSION     DATGEM010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267699"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..897)
                     /locus_tag="VKQ27_19260"
     CDS             complement(127..897)
                     /locus_tag="VKQ27_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91131.1"
                     /translation="MAGEVQDAIIRVRDISVQFGATKVLDGLNLDVKRGEILGFVGPS
                     GAGKSVLTRTIIGLVPKISGRIEVFGVDLDAASAAERRGVERRWGILFQQGALFSSLT
                     VRQNIQFPVREYLNVTQRLLDEITVAKLGMVGLRPEVADRFPSELSGGMVKRVALARA
                     LALDPELVFLDEPTSGLDPIGAGDFDELVRTLQRTLGLTVFMVTHDLDSLYTACDRIA
                     VLGNGKIIAAGSMADMQASQHPWLRAYFHGKRARAVMG"
     gene            complement(900..2033)
                     /locus_tag="VKQ27_19265"
     CDS             complement(900..2033)
                     /locus_tag="VKQ27_19265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91132.1"
                     /translation="MNGDPTLEWIARGNGLALCAAGVWTARFAPVLEAMVADAERLTG
                     SRPNIFIDVSQVSKLDTFGAWLIERLRRSLTQGEVEAKIAGLSANYSSLVDEVRRVKA
                     APAADATSVTITGMLDQIGRSMAGIAGTVIGLIDMLGAVLAASARVLIHPRAIRLTSI
                     VHHLEQVCWRAVPIIVLITFLIGCIISQQGIFHFRKFGADIFVVDMLGVLVLREIGVL
                     LVAIMVAGRSGSAYTAELGSMKMREEIDALRTMGFDPIEVLILPRMLALIIALPILAF
                     LGAMAALYGGGLVAWLYGGVEPEAFLLRLRDAISIDHFTVGMIKAPVMAAVIGIVACV
                     EGLAVQGSAESLGQHTTSSVVKGIFFVIVMDGVFAIFFASIGM"
BASE COUNT          478 a          710 c          617 g          347 t
ORIGIN      
        1 gtgaatgctc cgatcagaac gaaattcgcc cgcgtttcca ttcaaaccaa ctccgctacc
       61 gaccaaattg ggccaagaca ccccaagatg ctggcccgaa gacactggct catgatgccc
      121 aacacactag cccatgacgg cgcgggcgcg cttgccatgg aagtaagccc tcaaccaggg
      181 atgctgcgag gcctgcatgt cggccatcga tcctgcggca atgattttac cgttccccaa
      241 aacggcaata cggtcacacg cggtgtaaag gctgtccaaa tcgtgggtta ccatgaaaac
      301 ggtcaggccc aaagtgcgct gaagggtccg caccagctca tcgaagtcgc cagcgccgat
      361 cggatcgagg cccgaggtcg gttcatccag gaacaccagt tccggatcga gcgcgagcgc
      421 acgcgccagc gccacacgct tgaccatgcc gcctgacagt tccgacggga aacggtcggc
      481 gacctcgggg cgcaatccaa ccatgccgag cttggccacc gtgatctcgt cgagcaggcg
      541 ctgggtgacg ttgagatatt cgcgcaccgg aaactggatg ttctgtcgca ccgtcagcga
      601 ggaaaaaaga gcgccctgct gaaacaggat gccccaacgc cgctcgaccc cgcgccgttc
      661 cgcggcactc gcggcgtcca gatcgacccc gaacacttcg atgcgcccgg atatctttgg
      721 aaccaaaccg ataatggtgc gcgtcaatac cgacttgccc gcgccggagg gtccgacgaa
      781 gcccagaatc tcgccgcgct tcacgtcgag attgaggccg tcgagaacct tggtggcgcc
      841 gaactgcacc gagatatcgc gcacccggat gatcgcgtcc tgcacttcac cggccatcgt
      901 cacattccaa tcgaggcgaa gaagatcgca aacacgccgt ccatcacgat gacgaagaag
      961 atacctttca cgaccgagga cgtggtgtgt tggccgagcg attccgcgct gccctgcacc
     1021 gcgagcccct cgacgcaggc gacgattccg atcaccgccg ccatcaccgg tgccttgatc
     1081 atgccgacgg tgaagtggtc gatcgagatc gcatcgcgca gtcgaagcag gaatgcttcg
     1141 ggctcgacgc cgccgtagag ccacgccacc agcccgccgc catagagcgc cgccatggcg
     1201 ccgaggaagg ccaggatcgg cagcgcgatg atcagcgcca acatgcgcgg caggatcagc
     1261 acctcgatcg ggtcgaagcc catggtgcgc agcgcgtcga tctcctcccg catcttcatc
     1321 gagccgagtt cggcggtgta ggcactcccc gagcggcccg ccaccatgat ggccaccagg
     1381 agcacgccga tttcgcgcag caccaggaca cccagcatgt ccaccacgaa gatatcggcg
     1441 ccgaatttgc ggaaatgaaa gatgccttgc tgggagatga tgcagccgat caagaaggta
     1501 atcagcacga tgatcggcac cgcgcgccag caaacctgtt ccagatgatg cacaatagaa
     1561 gtcaaccgaa tggcccgcgg atggatcaga acccgcgcac ttgcagccag cactgcgccc
     1621 agcatgtcga tcaggccgat cacggtgccg gcaatgccgg ccatgctgcg gccgatctga
     1681 tcgagcatgc cggtgatcgt taccgacgta gcgtcggccg cgggtgcggc tttcacacgc
     1741 cgcacctcat cgaccaggct cgagtaattc gcagaaagcc cggcgatttt cgcctcgacc
     1801 tcgccctggg tcaggctgcg gcgcagccgc tcgatcagcc aagcgccgaa cgtatcgagc
     1861 ttggagacct gcgacacatc gatgaagata ttgggccggc ttccggtcag cctttcggca
     1921 tcggccacca tcgcctcaag cactggcgca aagcgggcgg tccagacgcc cgccgcacac
     1981 aacgcaagcc cattgcccct tgcaatccat tccaatgtcg ggtcgccgtt cacgatgcca
     2041 actcctgcgc cgattgttgc ctagagcacg atccggaaaa gtggaaaccg gtcctccgaa
     2101 aagaccgtgc tcaaacaagg agatgggatc atgatgcgat tcgatttaat cg
//
LOCUS       DATGEM010000625         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_471119, whole genome shotgun sequence.
ACCESSION   DATGEM010000625 DATGEM010000000
VERSION     DATGEM010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471119"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VKQ27_19270"
     CDS             complement(<1..299)
                     /locus_tag="VKQ27_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HLY91133.1"
                     /translation="MTPLAATDKLFFERAEAALDRGAAEATLDRALAHGDDGELYLEY
                     RESEQISLDDGRIRSAGFDTSLGFGLRAVADEATGYAHAGELSEAALGRAADSVAA"
     gene            complement(340..2628)
                     /locus_tag="VKQ27_19275"
     CDS             complement(340..2628)
                     /locus_tag="VKQ27_19275"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HLY91134.1"
                     /translation="MTDGVPPTPLLTDGVLEAMPVGVAVVDADKRLVLFNSAYYESLD
                     LPPNSYYRGMPVADALRVSAYRGVYGPGDPEAQVSTYMDIDRSRPGRLRRRTFRGRSF
                     DVLQAPLPAGGYVVCAVETTALVTARATAETALSRVNTALTTLRTGLAAFGPDSRLLF
                     ANPRFIELFGLTVEQPAPGTPFLELLDALADREEFAAVDNAAFLDSQRVLDRSHPSSM
                     RRVLPSGGVIDIVSDPLPDGGWTMAITEISPLVRAEDEARRRAGMLQSIVDAVPHGIA
                     VYSADRRVTMFNRAYSDVMSGAPLSVGDHLIDVIRRRAAAGEYGAGDPEQIVLQQLAF
                     DLTRPQNRKRQRPNGTMVDVRTKPLPDGGYISVVTDISPLTAVEAELSRRAEDLAVML
                     SNIRQGVLLWGPDRRLIASNPTASELLAQSPGQLAPGESQDAVLENMLARGWFGGGSG
                     ASAEVEALRSADNTMPFRRQLYSSSGRVLDFRSDPTPTGGWVSTFTDVTEARAAEDEL
                     RRAKEAAEAANHAKSRFLATMSHELRTPLNAVIGFSDALLQDATNPSRERIVEYAGQI
                     NDAGRQLLGLINIILDVARIESGRVDFASDTVDIARVIRNSVRQADTAAQAAEITLVT
                     DLPGDLPAVLADERRLQQALSHLLSNAVKFTEPGGTVTVGASIAEDGRLLLFVRDTGI
                     GIPEQDLERVFEPFIQLDGSLARRFQGAGLGLYMSRALVHGHGGELTLRSLPGAGTHA
                     EICLPAERVLPQGQALVVGSQV"
     gene            complement(2636..3730)
                     /locus_tag="VKQ27_19280"
     CDS             complement(2636..3730)
                     /locus_tag="VKQ27_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLY91135.1"
                     /translation="MPFDVREFPGAPSEDRAAGRRARRLVASWLFGIAFMVLAMIVLG
                     GITRLTGSGLSIMEWDPIMGALPPMSHAEWQRLYHLYQQIPQYTLVNEGFGLDGFKQI
                     FWLEWTHRLWGRLIGFAFLVPLIWFWVTGRIERRLRPFLVLLFMLGALQGAVGWFMVA
                     SGFFPETTAVSPVRLEIHLGLALLLYVALIWTGLSVLRPDPDHMAHGRLLHRLAWLCC
                     GFIGITMLAGGFVAGTHAGFDYNTFPLMDGQLVPPAYAQLQPFLRNLTENIAAVQFDH
                     RLLATLTVLAVAGTFAIGLFLRLPPWPRFGLLALGAAGAAQYLLGVATLLSVVQVDLA
                     AMHQAMAVLLLTSAIVLLHATRPSPLAARR"
BASE COUNT          765 a         1283 c         1323 g          648 t
ORIGIN      
        1 gcagcgacgc tgtccgccgc gcggcccagc gctgcctcgg acagttcccc ggcatgggca
       61 tagccggtcg cctcgtcggc caccgcgcgc aaaccaaagc caagcgaggt atcgaacccg
      121 gcgctgcgga tccgtccgtc atccaggctg atctgttcac tctcccggta ttccaggtaa
      181 agctcgccgt cgtcgccgtg cgccagcgcc cggtcaagcg tggcctcggc cgcgccgcga
      241 tcaagtgcgg cctcggcgcg ctcaaaaaac agcttgtcgg ttgcggcgag cggcgtcatg
      301 gcgcgggttc cttgaatagc tctcgtattc agagtggggt cacacctggc tgccgacaac
      361 caatgcctgg ccctgcggaa gcacgcgctc ggcgggcagg cagatttccg cgtgcgtgcc
      421 ggcacccggg aggctgcgca gtgtcagttc gccaccgtgg ccgtggacca acgcacgcga
      481 catatagagc ccaagccccg cgccctggaa tcgccgtgcc aggctgccat cgagctggat
      541 gaacggttcg aaaacccgct ccaggtcctg ctcggggatg ccgatgcctg tgtcgcgtac
      601 gaacagcagt agacggccgt cctcggcgat cgaggcgccg acggtcacgg ttccccccgg
      661 ctcggtgaac ttgaccgcgt tcgacagcaa gtggctgagc gcctgctgca accgccgttc
      721 atccgccagg acggcgggaa ggtcgccggg cagatcggtg accagcgtga tctcggctgc
      781 ctgagcggcg gtgtcggcct ggcgcacgct gttgcgaatg acccgggcaa tgtccacggt
      841 gtccgaggcg aaatcgaccc gtcccgattc gatgcgcgcc acgtcgagga tgatattgat
      901 cagcccaaga agctggcgtc ccgcgtcgtt gatctgcccg gcatactcca cgatgcgctc
      961 gcgtgacggg ttggtggcat cctggagcag cgcgtcggag aagccgatca ccgcgttcaa
     1021 tggcgtgcgc agttcgtggc tcatcgtggc caggaagcgg gacttcgcgt gattggcggc
     1081 ctcggcggcc tccttggcac gccgcaactc gtcctcggcg gcgcgcgcct cggtaacgtc
     1141 ggtaaaggta ctgacccaac caccggttgg cgtcgggtcg gaccggaagt ccagcacgcg
     1201 tcccgaactg gaataaagct gtcgccggaa cggcattgta ttgtcggcgc tgcgcagggc
     1261 ctctacttcg gctgacgcgc cggagccacc gccgaaccag ccgcgggcga gcatattctc
     1321 cagcaccgca tcctgcgatt cgcctggcgc caactgaccg ggtgactgcg ccagcaactc
     1381 cgatgccgtt ggattgctgg cgatcaggcg ccgatccggt ccccacagca ggacgccctg
     1441 gcggatattg gaaagcatca cggccaagtc ttccgcccga cgcgacaatt cggcttctac
     1501 cgcggtgaga ggcgagatgt cagtaacgac gctgatatag ccgccatccg gcagcggctt
     1561 ggtacgcacg tcgaccatag tgccgttcgg ccgctgccgt ttgcgattct gcgggcgcgt
     1621 cagatcgaac gccagctgtt gcaggacgat ctgctcggga tcgccggcgc cgtattcgcc
     1681 ggcagcggcg cggcggcgga tcacatcgat cagatggtcg ccgacggaca acggcgctcc
     1741 ggacatcacg tcggaatacg cccgattgaa catcgtgacg cggcggtcgg cgctgtagac
     1801 ggcgatgccg tgcgggaccg catccacgat cgactgcagc atgccggcgc gtcgccgggc
     1861 ctcgtcctcg gcacgcacca gcggactgat ctcggtgatc gccatcgtcc agccgccgtc
     1921 gggcagcggg tcggagacga tatcgatcac cccgccgctc gggagcactc gtcgcatcga
     1981 cgaagggtgc gatcgatcga gcacgcgttg agagtcgagg aaggcggcgt tgtcgaccgc
     2041 ggcgaattct tcgcggtccg ccagcgcatc gagcagttcg aggaacggcg tgccgggtgc
     2101 cggttgctcg accgtaagtc cgaacagctc gataaatcgc gggttggcga acagcaggcg
     2161 gctgtcgggg ccgaacgcgg cgagtccggt gcgcagcgtc gtcagcgccg tattgacgcg
     2221 cgagagcgct gtttccgctg tcgcacgcgc cgtgaccaac gccgtggttt ccaccgcaca
     2281 cacaacgtag ccgcccgcgg gcagcggggc ctgaagcaca tcgaagctgc ggcccctgaa
     2341 cgtccggcga cgcagccgac cggggcgcga gcgatcgatg tccatatagg tagagacctg
     2401 cgcctcggga tcgcccggtc catatacgcc gcgataggcg gagacgcgca aggcgtctgc
     2461 gaccggcatg ccgcggtagt agctgttggg cggcaggtcc agcgattcat aataagcgct
     2521 gttgaacagg accagccgct tgtcagcatc gacaacggca acgccgacag gcatggcttc
     2581 cagcacgccg tcggtcagca atggcgtagg cggaactccg tcggtcattg cagtctcatc
     2641 ggcgtgccgc cagcggagag ggacgcgttg cgtgcagcag cacgatcgcg ctggtgagca
     2701 gcagcacagc catcgcctga tgcattgcgg cgagatcgac ctgcaccacg gacaggagag
     2761 ttgccacacc gagcagatac tgtgcggcgc ccgccgcgcc gagggcgagc agcccgaaac
     2821 gcggccatgg cggcaacctg aggaacagcc cgatcgcgaa tgtgccagcg accgccaata
     2881 ctgtcagcgt ggccaagagc cgatggtcga actgcactgc cgcaatattc tccgtcaggt
     2941 tgcgcaagaa cggctgtagt tgggcgtatg caggcggcac gagctggcca tccatcagcg
     3001 ggaatgtatt gtaatcaaat ccagcgtgag ttccagcaac gaagccgcct gccagcatag
     3061 taatgccaat aaatccgcaa cacagccacg caagccggtg cagcagtcgg ccatgtgcca
     3121 tgtggtcggg gtcagggcgc agcacgctga ggccggtcca gatgagcgca acatatagca
     3181 gcaacgccaa tccaaggtgg atctcgagcc gaacgggtga aacggctgtt gtctcgggga
     3241 agaagcctga tgccaccatg aaccagccca ccgccccttg cagcgcgccc agcatgaaga
     3301 gcaacaccag gaacggccgc aacctgcgct cgatccggcc ggtgacccag aaccagatca
     3361 gcgggacgag gaacgcaaag ccgatcaatc gtccccaaag gcggtgggtc cattcgagcc
     3421 agaagatctg cttgaagccg tcaagaccga agccctcatt gacgagtgtg tattgcggaa
     3481 tctgctgata gaggtggtac aggcgctgcc attccgcgtg gctcattggc gggagtgcgc
     3541 ccatgatcgg gtcccattcc atgatcgaga gcccggaccc ggtgagacgg gtgataccgc
     3601 ccagcacgat catcgccagg accatgaagg cgatgccgaa cagccaactc gcgaccaggc
     3661 ggcgagctcg gcggccagcc gcccggtctt ccgatggcgc acctgggaac tcacgaacgt
     3721 caaagggcat ggcgctatat aggctcaagc gcctgctgcc gaaactccgg caggcgtgtc
     3781 agatggccag agtgggatga cgtcagctgg ggttacagtt gccctggttt cgcgcgtcgc
     3841 gccctgtccg actggaagag aatggaaccg ccgcaaagca cgcgaaatac gcaaaaggcg
     3901 gtgtgttgcg gggcaaggac aatgcgtaag cttggccctg ccaggatgca tttgcgggtt
     3961 cagcgtgttt cgcggttcca aatatcattg ttttctgccg gcacacgcct gacaggtcg
//
LOCUS       DATGEM010000626         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_156595, whole genome shotgun sequence.
ACCESSION   DATGEM010000626 DATGEM010000000
VERSION     DATGEM010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156595"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VKQ27_19285"
     CDS             complement(<1..295)
                     /locus_tag="VKQ27_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91136.1"
                     /translation="MLIGELLDVGGRGVAQAIVGASLLVLVLLGAALLVPVLILTSLL
                     PTGTPSAVAALPRAVPAVPQLPFGATVGTSRGTTLPAPLGGDIIDVARRYLGVP"
     gene            complement(303..1334)
                     /locus_tag="VKQ27_19290"
     CDS             complement(303..1334)
                     /locus_tag="VKQ27_19290"
                     /inference="COORDINATES: protein motif:HMM:NF039657.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL family protein"
                     /protein_id="HLY91137.1"
                     /translation="MIRLVVRLVGVLVATLAVAALGDVALAQPIQLPAPAGLDKLLAN
                     PGQWLSDMFNSAVLAIGSKTTDDVVQFMTSLGSGNIVLRTPPELTYQNGAVKTLSDGM
                     VQAADFALAAITVIGGINLIVHPHIRAPYHGALELIPRVLLGGILIHTSLGWGRFVIE
                     LNNAICGAISGVSMPDWSSFARLPTDGSVLLNLVAIAIYLVMGLLLVGQMLMRLALVD
                     ALLVIAPLALLCWVLPQTYAWARLWFTTFFGTVFVQSIQVLVLRLGAELMQSLVPMLG
                     SFGSDPVGTAHAWLMTLLLGIAVLQLTRKIPRLVQGVSSGFGPAYAGPSITQLAGLFG
                     SNKNRKGSK"
     gene            1547..>2052
                     /locus_tag="VKQ27_19295"
     CDS             1547..>2052
                     /locus_tag="VKQ27_19295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY91138.1"
                     /translation="MAFDLVISGGTLVNPSGRRRAHVYVQDGVIAAVTDADLESHRRI
                     DASGLYVLPGAVDGHVHFQDPGDTSREDFITGSSAAAVGGVTTLVEHTHSHPVRNVAF
                     LREKIEHLRKRSVVDYGLAAHAWPDQPSQSSELWRAGVTFFKVFTCTTHGVPGHDAAA
                     LLALFRELA"
BASE COUNT          417 a          648 c          674 g          313 t
ORIGIN      
        1 acggcacgcc gagatagcgg cgcgcgacgt cgatgatgtc gccgccaagt ggcgctggca
       61 gagtcgtgcc acgagaggtt ccgacggtcg ctccgaacgg tagctgcggc accgcgggta
      121 cagcgcgtgg caacgcggcc acggctgacg gcgtgccggt gggtagcagg ctcgtgagga
      181 tcagtaccgg caccagcagc gccgcgccga gcagcaccag gaccaggagc gacgcgccga
      241 cgatcgcttg agcaacaccg cgaccgccga cgtcgagcaa ctctccgatc agcatgggat
      301 gatcacttgc ttcctttgcg gttcttgttg gagccgaaca gaccggcaag ctgggtgatg
      361 ctgggtccgg cgtaggccgg gccgaagccc gacgagacgc cctgcaccag gcgcggaatc
      421 ttgcgcgtga gctggagtac cgcgatcccg agcaacagcg tcatcaacca ggcatgtgcg
      481 gtaccgaccg ggtcggagcc gaacgagcca agcatcggca ccaggctttg catgagctcc
      541 gcaccgaggc ggagcacgag cacctgaatc gattgcacaa acaccgttcc gaaaaacgtg
      601 gtgaaccaca gccgcgccca cgcgtacgtc tggggcaaca cccagcacag caacgcgagt
      661 ggcgcaatga ccagcaaggc gtcaaccagt gccagtcgca tcaacatctg gccgaccagg
      721 agcagcccca tcaccaggta aatggcgatg gcgaccaggt tgagcagcac cgaaccatca
      781 gtcggaagcc gcgcgaagct actccagtcg ggcatcgaaa cgccactgat tgcgccgcaa
      841 atcgcgttgt tcaactcgat gacgaagcgg ccccagccaa gactggtgtg gatcaggatg
      901 ccgccgagga gcacccgtgg aatcagctcc agtgcaccgt ggtacggcgc gcgaatgtgc
      961 ggatgcacaa tcaggttgat gccgccgatg acggtaatcg ccgcaagcgc gaaatcggcg
     1021 gcctgcacca tgccatcgga cagcgtcttg accgccccat tctggtaggt cagctcgggc
     1081 ggtgtacgca gcacgatgtt gccgctgccg agcgacgtca tgaactgcac cacgtcgtcg
     1141 gtggtcttgc tgccgatggc gagcacggcg gagttgaaca tgtcgctcag ccactggccc
     1201 ggattggcga gcagcttgtc cagcccagcg ggtgcgggca gctggattgg ctgcgcgagc
     1261 gcaacatcgc caagcgcggc gaccgccaga gtggccacca acacgccgac gaggcggacc
     1321 accagacgga tcatcggccg attcccgatg cttgacatgc cgccagcatg cggcaagggg
     1381 cgtcacacgc agcgtcacgc ggagggtgaa aagcgtcacg tacgcacgaa aatgcgtcac
     1441 atttgcagca aagggcgtca catgcggcgg cggaagggtt gggcacgcgt cacacgggga
     1501 gagtcggttg cgacggcaat aattgccgta ctatccaggc ggatagatgg catttgacct
     1561 ggtcatctcg ggcggcacgc tggtcaatcc gtccggtcgg cggcgagcgc acgtctatgt
     1621 ccaagacggt gtgattgcgg cggttaccga cgcggacctg gagagccacc gccgtatcga
     1681 tgcctcaggc ctgtacgtgc taccgggcgc ggtggacgga cacgtacact ttcaggaccc
     1741 cggcgacacc agccgcgagg acttcatcac cggcagctcc gccgcggccg ttggcggagt
     1801 aaccaccctt gtcgagcaca cgcacagtca tccggtgcgc aatgtcgcct ttcttcgaga
     1861 gaagatcgaa caccttcgca agcgatcggt ggttgattac gggctggcgg cccacgcctg
     1921 gccggaccaa cccagccagt cctccgaact gtggcgggcc ggagtcacgt ttttcaaggt
     1981 cttcacgtgt accacgcatg gcgtgccggg tcacgacgcg gcggcgctac ttgcgttgtt
     2041 tcgcgagctg gc
//
LOCUS       DATGEM010000627         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1300700, whole genome shotgun sequence.
ACCESSION   DATGEM010000627 DATGEM010000000
VERSION     DATGEM010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300700"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /gene="atpG"
                     /locus_tag="VKQ27_19300"
     CDS             <1..714
                     /gene="atpG"
                     /locus_tag="VKQ27_19300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HLY91139.1"
                     /translation="KIIGHLRQVNLDYKHPFTLERPVRAVGIVVISTDRGLCGSLNLN
                     LFKAALGAIREAQAKNAKVYVCAIGSKALGFFRRLSGIEVSASVTHLGDKPHVAGLIG
                     TTKVMLDFYRDAKVDQLLLVHNVFVNTMTQKGVVSQLLPLQTIDKEELQERWDYLYEP
                     DSSEILEGILMRYIESQVFQGAVENVACEMAARMVAMKSASDNAGKLIDELQLIYNKA
                     RQAAITKELSEIVGGAAAV"
     gene            902..2323
                     /gene="atpD"
                     /locus_tag="VKQ27_19305"
     CDS             902..2323
                     /gene="atpD"
                     /locus_tag="VKQ27_19305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLY91140.1"
                     /translation="MLSSGKIVQIIGAVIDVEFPRDQIPKVYDALKLDDVDLTLEVQQ
                     QLGDGIVRTIAMGPSEGLRRGLTVNGTGKGISVPVGAGTLGRIMDVLGKPQDEKGPVQ
                     ATEYLPIHRPAPAYDEQAGGQDLLVTGIKVIDLLVPFAKGGKVGLFGGAGVGKTVNML
                     ELINNIAKQYSGLSVFAGVGERTREGNDFYHEMIESGVIDKDNLANSKVAMVYGQMNE
                     PPGNRLRVALTGLTMAEYFRDEQQASGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPS
                     AVGYQPTLAEEMGVLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVL
                     SRQIASIGIYPAVDPLDSTSRQLDPLVVGQEHYDTARGVQAVLQRYKELQDIIAILGM
                     DELSEEDKLSVYRARKIQRFLSQPFNVAKVFTGQDGKIVPLKDTIRGFKAIIAGEYDH
                     LPEQAFYMIGAIEEAVEKAKTLA"
     gene            2332..2751
                     /locus_tag="VKQ27_19310"
     CDS             2332..2751
                     /locus_tag="VKQ27_19310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF001847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HLY91141.1"
                     /translation="MAGTIHVDIVSAEGQIFSGEATMVFAPGSLGELGIAPRHAPLLT
                     TLKAGEVRVQSEGQDEQSFYVGGGTLEIQPHLVTVLADTAARAHDLDEAAALAAKQRA
                     EDAVRERTDKVDIAEAQAELARAMAQLRAIERLRKRR"
     gene            2816..>3876
                     /gene="glmU"
                     /locus_tag="VKQ27_19315"
     CDS             2816..>3876
                     /gene="glmU"
                     /locus_tag="VKQ27_19315"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLY91142.1"
                     /translation="MPLSVVILAAGQGKRMHSDLPKVLQPLAGEPLLQHVLRSARALA
                     PTNVYVVYGHGGAQVQQAFAGEDVDWVLQAEQLGTGHAVMQAMCLIPDDHTVLVLYGD
                     VPLIRPACLEKLVASAGSGALALLTVNLDQAAGYGRIVRGADGHVTAIVEHNDATPEQ
                     LSIREANSGLMAAPAGPLRDWLLGLGSANAQREYYLTDVVAGAVQRAYPVEAIPVARA
                     VEVLGVNDRLQLAQVEAGLRLLRTEELMLAGATLADPLRVDVRGTVTVERDVFIDVGA
                     VLIGQVHLGARVTVGPNCLIRDSRIGPDTEINANCVIEGAQVADHCRIGPFARLRPGA
                     AVERNAHVGNFVEIKNAKIG"
BASE COUNT          726 a         1230 c         1264 g          656 t
ORIGIN      
        1 aagatcatcg gccatctgcg ccaggtgaac ctcgactaca agcatccctt caccctggag
       61 cgcccggtgc gagcggtcgg catcgtcgtc atcagcaccg accgggggct gtgcggctcg
      121 ctcaatttga atctgttcaa ggccgcgctc ggcgcgatcc gcgaggccca ggcaaagaac
      181 gccaaggttt acgtctgcgc gatcggctcg aaggccctgg gattctttcg acgcctgtcc
      241 ggaatcgaag tgtcggcttc ggtcacgcac ctgggcgaca agccgcacgt cgccggattg
      301 atcggcacca ccaaggtcat gctggatttc tatcgcgacg ccaaggtcga ccagctgctg
      361 ctggtgcaca acgtgttcgt caacaccatg acgcaaaagg gtgtggtttc gcagctgctg
      421 ccgcttcaga cgatcgacaa ggaagaactg caggagcgct gggactatct ctacgagccg
      481 gattccagcg agatactcga gggcattctg atgcgctaca tcgaatccca ggtctttcag
      541 ggcgcggtcg agaatgtcgc atgcgaaatg gcggcgcgca tggtggccat gaagagcgcc
      601 tccgacaacg ccggcaagct catcgacgag ctgcagctca tttacaacaa ggcccgccag
      661 gccgcgatca cgaaagagct ttccgagatc gtcggcggcg ccgccgccgt ttagatcggg
      721 tcaggcacaa gttaaggttt aggtataaag gtcgagttta ggtacacgga ttaggtctta
      781 tcggcgcgca gcgccgctaa tgctcggggt tgcgcagcaa ccccgttgaa agttggccga
      841 aggccttgaa taggtggccg aaggccatga aaatttggcc gtcaggccat gaggtaagta
      901 catgttatcc agcggcaaaa tcgtgcaaat catcggcgcg gtcatcgatg tggagtttcc
      961 ccgcgaccag atccccaagg tctatgacgc gctcaagctc gatgatgtgg acctgacgct
     1021 cgaagtgcag cagcagctgg gcgacggcat cgtgcgtacg atcgccatgg gtccgtccga
     1081 gggtctgcgc cgcggtctca ccgtcaacgg gacgggcaag gggatttccg tgccggtggg
     1141 agcgggcacg ctcggccgca tcatggacgt gctcggcaag ccgcaggacg agaaggggcc
     1201 ggtgcaagcg accgagtatc tgcccattca ccgtcccgct ccggcctacg acgagcaggc
     1261 gggcggtcag gaccttctgg tcacgggcat caaggtgatc gatctgctgg tgcccttcgc
     1321 caagggcggc aaggtgggcc tgttcggcgg cgccggcgtc ggcaagaccg tgaacatgct
     1381 cgagctcatc aacaacatcg ccaagcagta cagcggtctg tcggtgtttg ccggcgtcgg
     1441 tgaacgcacc cgcgagggca atgacttcta tcacgagatg atcgaatcgg gcgtcatcga
     1501 caaggacaat ctggccaatt ccaaggtggc catggtgtac gggcagatga acgagccgcc
     1561 gggcaaccgc ctgcgcgtcg cgctcaccgg tcttaccatg gccgaatact tccgcgacga
     1621 acagcaggcc tccggcaagg gccgcgacgt gctgttcttc gtcgacaata tctaccgcta
     1681 caccctcgcg ggcacggagg tctcggcgct gctcgggcgc atgccctccg cggtgggata
     1741 tcagccgacg ctggccgagg aaatgggcgt gctgcaagaa cgcatcacct ccaccaagac
     1801 cggttcgatc acctccatcc aagccgtgta cgtccccgcc gacgacctga ccgacccctc
     1861 cccggccacg acgttcgccc acttggacgc caccgtggtg ctgtcgcggc agatcgcctc
     1921 gatcggcatc tatcctgcgg tcgaccctct ggattccacc agccgccagc tcgatccgct
     1981 cgtggtgggc caggagcact atgacaccgc acgcggtgtg caggccgtcc tgcagcgcta
     2041 caaggagctg caggacatca tcgcgattct cgggatggac gagctgtcgg aggaagacaa
     2101 gctctccgta taccgcgcgc gcaagattca gcgcttcctg tcgcagccct tcaacgtggc
     2161 caaggtgttc accgggcagg acggcaagat cgtccccctc aaggacacca ttcgcggctt
     2221 caaggccatc atcgccggcg aatacgatca tctgcccgag caggcgttct atatgatcgg
     2281 cgccatcgaa gaagcggtcg aaaaagccaa gaccctggct tgagagagat catggccggc
     2341 accattcacg tcgacatcgt cagcgccgaa ggacagatct tttcgggcga ggcgaccatg
     2401 gtgttcgcgc cgggctcgct gggcgaactc ggcatcgccc cgcgccacgc gccgctcctg
     2461 acgacgctca aggccggcga agtgcgcgtg cagtcggaag ggcaggacga acagtcgttc
     2521 tacgtcggcg gcggcaccct cgaaattcag ccgcatctgg tgacggtgct ggccgatacg
     2581 gcggcccggg cacatgatct cgacgaagcg gcggcgctcg cggcgaagca gcgcgcggaa
     2641 gacgccgtgc gcgagcgtac cgacaaggtg gatatcgccg aggcgcaggc cgaactggcg
     2701 cgggccatgg cgcaattgcg ggcgatcgaa cggctgcgca agcgccgctg atgtaggaag
     2761 cttccgcccg acgcggctcc gcggaaatac gtaagatagg ctccgaggag tagtcatgcc
     2821 gctttccgtc gtcatactcg ctgcgggcca gggcaaacgg atgcactcgg atctgccgaa
     2881 ggtgctgcag ccgctggcgg gcgaacccct cctgcagcac gtgctgcgtt cggcacgcgc
     2941 gctcgccccg acgaacgtat acgtggtcta tgggcatggc ggcgcgcaag tccagcaggc
     3001 gtttgccggc gaagacgtcg attgggtgct gcaggccgag cagctcggca ccggccacgc
     3061 cgtgatgcag gccatgtgcc tgattcccga tgaccatacg gttctggtgc tgtacggcga
     3121 cgtgccgctg atccggcccg cctgcctcga gaagctggtg gcttccgccg ggtccggcgc
     3181 gctcgcattg ctcacggtca atctcgacca ggccgccgga tacggccgga tcgtgcgtgg
     3241 ggccgacggc cacgtgaccg ccatcgtcga gcacaacgac gcaactcccg agcagctctc
     3301 gatccgcgag gccaactcgg gtctcatggc cgccccggcc ggccccttac gcgactggct
     3361 gttggggctt ggatccgcca acgcgcagcg cgagtactat ttgaccgatg tggtggccgg
     3421 ggcggttcag cgcgcatatc cggtcgaggc cataccggtg gcgcgtgcgg tggaagtgct
     3481 cggcgtcaat gatcgcctgc agctcgcgca ggtggaggcc ggactgcggc tgctgcgcac
     3541 cgaggaactg atgctcgcgg gcgcgacgct cgccgatccg ttgcgagtgg atgtgcgcgg
     3601 caccgtcacc gtggagcgcg acgtcttcat cgacgtgggc gccgtgctga tcgggcaggt
     3661 gcacctgggc gcgcgcgtca cggtcgggcc gaattgcctg attcgcgatt cgcgcatcgg
     3721 ccccgatacc gagatcaacg ccaactgcgt catcgaaggg gcacaggtgg cggaccactg
     3781 ccgcatcgga cccttcgccc gcctgcgccc cggcgccgcc gtggagcgca atgcccatgt
     3841 gggaaacttc gtcgaaatca agaacgccaa gatcgg
//
LOCUS       DATGEM010000628         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1296731, whole genome shotgun sequence.
ACCESSION   DATGEM010000628 DATGEM010000000
VERSION     DATGEM010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296731"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VKQ27_19320"
     CDS             complement(<1..229)
                     /locus_tag="VKQ27_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91143.1"
                     /translation="MFAVITPSPPDHHRPAVMAGLTRPSVAIGRTAGHRAHPFQQRSN
                     LTCDSRFVPNLARHFTAKRAAAMDVNTAEASL"
     gene            326..1408
                     /locus_tag="VKQ27_19325"
     CDS             326..1408
                     /locus_tag="VKQ27_19325"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY91144.1"
                     /translation="MVSTRRKLETRSHLLMHARSLVTAAAILLAMSFAAAQARDLTIV
                     ALAPAQSAIRQVFAKPFGEATAITTNVISWGGDMDMLRGQVKTPDSTWDVVMVDSEGL
                     AAGCSENLFEKLDWSAIGGKDHYLPQGVSDCGVGAGVASLALAWDRDKSPTPPTWADF
                     WDVARFPGKRGLHNGVRGNLEFALLADGVAPGDVYKVLATSEGVDRAFRKLDQLKPYI
                     VWWNDDAEAARILASGDVLMSSAFAPQIAQVNRTAHRNFGIQWAASLYEVQSWAIVKG
                     SPDVRQATQFLYFAGTPAIEARLVGQFGDGGLAKGANDGLPGELAQLSPTTPANLNAA
                     VRMDVGFWHDNLTKLRQRFDAWLAAH"
     gene            1405..2508
                     /locus_tag="VKQ27_19330"
     CDS             1405..2508
                     /locus_tag="VKQ27_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91145.1"
                     /translation="MTEIAHLSIERRDGGTVLAFAGRLEAHAIGAMWRPAIHAAQHAR
                     GHSLSFDLRNVTFCDVGGASLLAAVETAYGAPVEAVGLAERAIGLLERARAAGKIEQR
                     ATSISPFTLRDVAAAAARLLAFSVTFIGETAVAFAELPRHRRMLRVPDLLGYVDSAGI
                     RSLPLVVLLGYLIGLILAFQSAVPMRQFGADIFVASLVAIALLRELGPLLAAVILAGR
                     TGSAFAAEIGTMKVNEEIDALTTMGLDPVTMLVLPRVIAAMCVMPVMTMTLDIAGLLG
                     MATVMRGFGYPFVAIARQVQEWVTAGDLYGGLFKAVCFGLVVAAIGCRAGLGTGVGPR
                     SVGLSATSAVVGGIVATIAVDGVFALVFYRLHL"
     gene            2505..3287
                     /locus_tag="VKQ27_19335"
     CDS             2505..3287
                     /locus_tag="VKQ27_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY91146.1"
                     /translation="MSEEPIIEVDHVAQRFGSRTIFRDVSLQVYPREVFVILGGSGCG
                     KSTLMKQLIGLLVPSAGHISLYGHAITGPGGEAALHTVQQRIGVMFQSGALFGSMTLL
                     ENVMLPLEMFTALPPEGREAVARVKLGLVGLGDAAGLMPAEISGGMARRAGIARAMAL
                     DPPLLLLDEPSAGLDPITSAGLDRLILDLRQDLGATFVVVTHELASIMAIADRCVMLD
                     RGAYPDAGGVIAEGKPRELRETSTHPIVRAFFRRELPGAVAA"
     gene            3287..4303
                     /locus_tag="VKQ27_19340"
     CDS             3287..4303
                     /locus_tag="VKQ27_19340"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLY91147.1"
                     /translation="MEGRALYLRVGVLILAGIALLLGLLWFIGGNRFRSGTVYESYFS
                     ESVQGLEVGAPVKYRGVTVGRVTDLGLVTAEYGEGTTLDLQNSTSRLVFVRYELDTRR
                     IGQVPDTQTAVALGLRSRLASQGLTGLAYLELDFVSPKQYPPLAVPWQPEAEYIPSMP
                     STLGQIQVAAEKLLDKLNAMDIDKLTDEASGLLKDLRADVATGDVHATLSEATALLRA
                     MHDAVTAADVPGLTADLRRTSAAVRDTVQGEKVQALLSNAGVAAARLADAAAKLPPLI
                     ATLQATTTRIGNGSADVEQGVIPVLRDVQASAQNLRELTDLLRRYPAGAFSEPPPRGG
                     GTPR"
     gene            4300..4896
                     /locus_tag="VKQ27_19345"
     CDS             4300..4896
                     /locus_tag="VKQ27_19345"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HLY91148.1"
                     /translation="MRRRMVLLGLAGLGGCSLLPSSNYIQRRDWPLDVQRPSVLPLRV
                     RGKVLLVRSIRSGPGLDVRGLQWLQRDGSIHVDFYEQWAVPPAQAVEDDVRQWLAGSG
                     LFNAVVAPGSRLDADYVLEGELQAFVADPGAGVARVSVTFVLLDQHPSPIKAVLQRTE
                     SAEARLTGDDPPAIAAGMRAAVVEVLEKIERDVAGVVR"
     gene            4959..>5541
                     /locus_tag="VKQ27_19350"
     CDS             4959..>5541
                     /locus_tag="VKQ27_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HLY91149.1"
                     /translation="MSIALYAFTCGELEGEFCRVMEGGEGRIAMPVPVFLIEHLKGRV
                     LFDSGMHPDCQHDPVGRIGERMAGLYTIRFHPGEEISARLVAMNRDPAGIDLIVNSHL
                     HFDHVGGNVLVPNATMIVQRREWEARLDPDIAVPHGYDARDYDVGHKLRLIDGEYDVF
                     GDGAVVCLPSYGHTPGHQSLRVRLDSGDTVLAAD"
BASE COUNT          811 a         1504 c         2208 g         1018 t
ORIGIN      
        1 tgaggctggc ttcagctgtg ttcacgtcca tggcggcggc ccttttggct gtgaagtggc
       61 gcgcaaggtt aggcacaaaa cgtgaatcgc aggttaagtt ggatcgctgt tggaagggat
      121 gggcccgatg gccggcggtc cggccgatcg cgactgatgg ccgggtcaag ccggccatga
      181 cggctgggcg gtgatggtcg ggcggtgacg gggtgatgac ggcgaacatg gcggggccat
      241 gacgaagggg tgaagtgcac ctggggccgc aggaagcgcg tgtcgcgtat ggggggacgg
      301 ctttcgtttc cagcagaatt cgcggatggt aagcacccgc cgcaagctcg aaacccggag
      361 ccacttactg atgcatgccc gctcgcttgt aaccgctgcg gcgatccttc ttgccatgtc
      421 gtttgccgcg gcgcaggcgc gcgatttgac tatcgtggca ctcgctccgg cgcaaagcgc
      481 cattcggcag gtgtttgcca agccgttcgg cgaagccact gccatcacga ccaatgtcat
      541 cagctggggc ggcgacatgg acatgctgcg cggccaggtg aagacgccgg acagtacctg
      601 ggacgtggtg atggtggaca gcgagggcct cgccgccggg tgttcggaga atctgttcga
      661 aaagctcgac tggtcggcca ttggcgggaa ggaccactac ctgccgcagg gggtgagcga
      721 ttgcggggta ggggcgggtg ttgcgtcgct cgcgctggca tgggaccgcg acaagtcgcc
      781 gacgccgcca acctgggcgg atttctggga tgtcgcgagg ttcccgggta agcgtgggct
      841 gcacaatggc gtgcgcggca atctggagtt cgcgctgctc gccgacggcg ttgcaccggg
      901 cgacgtttac aaggtgctcg cgacgtcgga aggcgtggac cgggcgttcc gcaagcttga
      961 ccagctcaag ccgtacatcg tgtggtggaa cgacgatgcg gaggcggcgc ggatcctggc
     1021 ctcgggtgac gtgctgatga gcagcgcttt cgcaccacag atcgcacagg tgaaccggac
     1081 ggcgcaccgg aatttcggca tccagtgggc ggcgagcctg tacgaggtgc agagctgggc
     1141 gatcgtgaag ggcagcccgg atgtgcgcca ggcgacgcag ttcctgtatt tcgccgggac
     1201 gccggcgatc gaggcgcggc tggtggggca gtttggcgac ggtgggctgg cgaaaggcgc
     1261 gaatgacggt ctgccggggg aactggcaca gctttcgccg accacgccgg ccaatctgaa
     1321 cgcggcggtg cggatggatg tcgggttctg gcacgataac ctgaccaagc tgcggcagcg
     1381 cttcgacgcc tggctggcgg cgcattgacc gagatcgcgc atctctcgat tgagcggcgc
     1441 gatggaggga cggtgctggc gtttgccggg cggctggagg cgcacgcgat cggggcgatg
     1501 tggcggccgg cgatccatgc ggcgcagcat gcgcgcgggc attcgctgtc gtttgacctg
     1561 cgcaatgtga ccttctgcga cgttgggggc gcctcgctgc tggcggcggt ggagacggcg
     1621 tacggcgcgc cggtggaggc ggtcgggctc gcggagcggg ccattgggct gctggaacgc
     1681 gcgcgcgcgg cggggaagat cgagcagcgg gcgacgtcga tctcgccgtt cacgctgcgc
     1741 gatgtggcgg ctgcggcggc gcggctgctg gcgttcagcg tgacattcat cggcgagacc
     1801 gcggttgcgt tcgccgagtt gccgcggcat cggcgcatgc tgcgggtgcc ggatctgctg
     1861 gggtatgtgg acagcgccgg gatccggtcg ctgccgcttg tggtgctgct cggctatctg
     1921 atcgggctga tcctggcgtt ccagtcggcg gtgccgatgc gacagttcgg tgccgatatc
     1981 ttcgtggcga gcctggtggc gatcgcgctg ctgcgggagt tgggaccgct gctggcggcg
     2041 gtgatcctgg cgggccgcac ggggtctgcg ttcgccgccg agatcggcac gatgaaggtg
     2101 aacgaggaga tcgatgcact gacgacgatg ggcctcgatc cggtgaccat gctggtgctg
     2161 ccgcgggtga tcgccgcgat gtgcgtgatg ccggtgatga cgatgacgct ggacatcgca
     2221 gggctgctgg gcatggcgac ggtgatgcgc gggttcggct atccgttcgt tgcgatcgcg
     2281 cgccaggtgc aggagtgggt gacggcgggc gatctgtatg gcgggctgtt caaggcggtg
     2341 tgcttcggcc tggtggtggc ggcgattggc tgccgcgcgg gtctgggcac cggcgtgggg
     2401 ccgcggtcgg tagggctgtc cgcgacgtcg gccgtggtgg gcggcatcgt cgcgacgatc
     2461 gcggtcgatg gcgtgttcgc tctggtgttc tatcggctgc acctgtgagc gaggagccga
     2521 tcatcgaggt ggaccacgtc gcacagcggt tcggcagccg cacgatattc cgtgacgtgt
     2581 cactacaggt gtatccgcgc gaggtgttcg tgatcctggg tgggtcgggg tgcggcaagt
     2641 caacgctgat gaagcagttg atcgggttgc tggtgccgag tgccgggcat atctcgttgt
     2701 atgggcatgc gatcaccggg ccgggtggcg aggcggcgct gcacaccgtg cagcagcgga
     2761 tcggggttat gttccagtcg ggtgcgctgt tcggctcgat gacgctgctg gagaacgtga
     2821 tgctgccgct ggagatgttc acagcgctgc cgccggaggg gcgagaggcg gtggcgcggg
     2881 tgaagctggg cctggtggga ttgggcgacg cggcggggct gatgccggcg gagatttcgg
     2941 gtgggatggc gcggcgggcg gggatcgccc gggcgatggc gctggatccg ccgctgctgc
     3001 tgctggatga gccatcggcg gggctggatc cgatcacctc ggcgggactc gatcgattga
     3061 tcctagacct gcgccaggac cttggggcga cgttcgtggt ggtgacgcac gaacttgcct
     3121 ccatcatggc cattgccgat cggtgcgtga tgctggaccg gggcgcgtat cccgatgctg
     3181 ggggcgtgat cgcggaaggc aagccgcgcg agctgcgcga gaccagcacg catccgatcg
     3241 tgcgggcgtt cttccggcgc gagctgcctg gggcggttgc tgcctgatgg aaggacgggc
     3301 gctctatctg cgcgtgggtg tgctgatcct ggctgggatc gcgctgctgc tcgggctgct
     3361 gtggttcatc ggcgggaacc ggttccgcag cggcacagtg tacgagtcgt atttcagcga
     3421 gtcggttcag gggctggagg ttggtgcgcc ggtgaaatat cgcggcgtga cggtggggcg
     3481 ggtgactgac ctggggctgg tgacggcgga gtacggcgag gggacgacgc tcgatctgca
     3541 gaacagcacg tcgcgcctgg tgttcgtgcg ctatgagctc gatacccgcc gaattggcca
     3601 ggtgccggat acgcagacgg cggtggcgct cgggctgcgg tcacggctcg cgtcgcaggg
     3661 gctgacgggg ctggcgtacc tggagctgga tttcgtcagt cccaagcaat acccgccgct
     3721 ggcggtgccg tggcagccgg aggctgaata cattccatcc atgcccagca cgctgggcca
     3781 aattcaggtg gcggcggaga agttgctgga caagctgaac gcgatggaca tcgacaagct
     3841 gaccgatgag gcgagcgggt tgctcaagga cctgcgggcc gatgtcgcga ccggggatgt
     3901 gcatgcgacg ctgagtgagg cgacggccct gctgcgcgcc atgcatgacg cggtgaccgc
     3961 ggcggatgtg cctgggctga cggcggatct gcggcggacg tcggctgcgg tgcgcgacac
     4021 ggtccagggg gagaaggtgc aggcgctgct ttccaatgcg ggggtcgcgg cggcgcgtct
     4081 ggcggatgcg gcggcgaaac tgccgccact gatcgctacg ctgcaggcga cgacgacgcg
     4141 tatcggcaac ggttcggctg atgtcgagca gggggtgata ccggtgctgc gggacgtgca
     4201 ggcttcggcg cagaacctgc gggaactcac ggatctgctg cggcggtatc cggcgggtgc
     4261 gttcagtgag ccgccgccgc gtggcggagg gacgccgcga tgaggcggcg catggtcctg
     4321 ctggggttgg ccggcctggg cggatgctcg ctgctgccgt cctcgaacta catccagcgg
     4381 cgggactggc cgctggatgt gcagcgtccg tcggtgctgc cgctacgggt gcgcggcaag
     4441 gtgttgctgg tgcgctcgat ccgctcaggg ccggggctgg acgtgcgcgg gctgcaatgg
     4501 ctgcagcgcg acggcagcat ccatgtggat ttctacgagc agtgggcggt gccgccggcg
     4561 caggcggtgg aagacgatgt gcggcagtgg ctggctgggt ccgggctgtt caatgcggtg
     4621 gtggcgccgg gcagccggct ggatgccgat tacgttctcg agggcgaact ccaggctttc
     4681 gttgctgatc cgggcgcagg ggtagctcgg gtaagcgtga cgttcgtgct gctggatcag
     4741 catccgtcgc cgatcaaggc cgtgctgcag cggacggaga gtgcggaggc gcggctgacg
     4801 ggcgatgatc cgccggcgat cgcggcaggg atgcgggcgg cggtggtcga ggtgctggag
     4861 aagatcgagc gggatgtggc gggcgtggtg cggtaggagg cgagcacaat gagccggctg
     4921 acgcctgaac tgaatgtgtc ggacctgggt tcgtcctcat gagcatcgcg ctgtacgcgt
     4981 tcacctgcgg cgagcttgaa ggcgaatttt gccgggtcat ggagggaggc gaaggccgga
     5041 tcgccatgcc ggtcccggtt ttcctcatcg aacacctgaa ggggcgcgtg ctgttcgata
     5101 gtggtatgca cccggattgc cagcacgatc cggtgggaag gatcggcgag cggatggccg
     5161 ggctctacac gatcaggttt catcctggtg aggagatcag cgcgcggttg gtggcgatga
     5221 accgcgatcc ggctggcatc gatctcatcg tcaattcgca tctgcacttc gatcatgtcg
     5281 gtgggaatgt gctggttccg aatgcaacga tgatcgtgca gcgccgtgag tgggaggcgc
     5341 ggctggatcc ggatatcgcc gtgccgcacg gctacgatgc gcgggattac gatgtgggcc
     5401 acaagctgcg gctgatcgat ggcgagtatg acgtcttcgg tgatggtgcc gtggtttgcc
     5461 tgcccagcta tggacatacg cctgggcacc aatcgctgcg cgtgcgactg gacagtggtg
     5521 atacagtgct cgccgccgat g
//
LOCUS       DATGEM010000629         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_514913, whole genome shotgun sequence.
ACCESSION   DATGEM010000629 DATGEM010000000
VERSION     DATGEM010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514913"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..979)
                     /locus_tag="VKQ27_19355"
     CDS             complement(215..979)
                     /locus_tag="VKQ27_19355"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91150.1"
                     /translation="MNISFDGKVALVTGAASGLGLATAKAFAQAGASVALADWHEDAV
                     RNAANELTAQGRKALGIRCDVADDAQVEAMVRQTVAAFGRLDAAYNNAGVQNVLAETA
                     DTTREDYDRVMGINLRGEWSCMKFELRQMRRQGSGAIVNCSSLGGLVGGAERGIYHAA
                     KHGVLGFTKSAALEYAARGIRINAVCPGLIWTPMADQMVASGQGDALKEMESRVPMGR
                     VGRPEEIASAVLWLCSDAASYVTGQSISVDGGFVMH"
     gene            complement(1020..2009)
                     /locus_tag="VKQ27_19360"
     CDS             complement(1020..2009)
                     /locus_tag="VKQ27_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003626672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY91151.1"
                     /translation="MQARMLGKNRLEVSAIGLGCMGLNYHRGPAPDRAAMIALVRAAV
                     ERGVTFFDTAEVYGPFTNEELVGEALEPFRKDVVIATKFGHDPAGARGSDGLDSRPER
                     IRQAVEGSLKSLRVEAIDLLYQHRVDPGVPIEDVAGAVKGLVAQGKVRHFGLSEAGAA
                     TIRRAHATHPVAAIQSEYSLWWRQPEADVLPACEELGIGFVPFSPLGRGFLTGKIDET
                     TTFGGNDNRTGLPRFTPAARKANRPVVDLLETIGAQKGATPAQIALAWLLAQKPWIVP
                     IPGTTKLDRLEENIAAAAIVLTRDELRRIEDAAAKITVIGDRYPEADAARTQR"
     gene            complement(2065..>2403)
                     /locus_tag="VKQ27_19365"
     CDS             complement(2065..>2403)
                     /locus_tag="VKQ27_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY91152.1"
                     /translation="VRIDPLFQAPDPARARGASVTFEPGARTAWHTHPLGQTLIVTAG
                     CGRVQRDGGPIEEIRPGDVVLFAPNEKHWHGAAPTTAMTHIALQEALNGKVVDWMEHV
                     SDAQYRAGAQ"
BASE COUNT          382 a          822 c          756 g          443 t
ORIGIN      
        1 ggccgtagcc agagctcagc cccatgcagc ccaggccgat cgcggaaact tcgagaccat
       61 cgctgccgag tttgcgtttc tgcatggtgt gctctcgtgt cgtttcatcc ggcgcgatga
      121 tcgcggccgt cgatcctgct ccgtcataag tccgtctaag ccccccgttc tgcgcgtcct
      181 gcggatgccg gggatcgccg agacgtccaa ccggtcagtg catgacgaaa ccgccgtcca
      241 ccgagatgga ttggccggtg acgtagctcg cagcgtcact gcagagccac agaacggcac
      301 tcgcgatttc ctcgggtcgg ccaacccgcc ccatcggtac acggctctcc atctccttga
      361 gggcgtcacc ttgaccggac gcgaccatct ggtcggccat gggcgtccag atgaggccag
      421 ggcagacagc gttgatgcgg atgccgcgtg ccgcatattc aagagctgca ctcttggtaa
      481 aaccgaggac tccgtgtttg gctgcgtggt agattccacg ctcggcgcct cccaccaggc
      541 cacccaggga agagcaattg acgatcgcgc cgctgccttg ccggcgcatc tgccgcaact
      601 cgaatttcat gcagctccat tcaccgcgga ggttgatccc catcacgcgg tcataatcct
      661 cacgggtggt gtcagccgtc tccgccagga cattctgcac gccggcgttg ttgtatgcgg
      721 catcaagccg gccgaaggcc gcgaccgtct gtcggaccat ggcttcgacc tgtgcgtcgt
      781 cggcgacgtc gcaacggatg ccgagagctt tacgaccctg ggcggtcagt tcgttggcag
      841 cgttacgcac cgcgtcctcg tgccagtcag ccagggcgac agacgcgccg gcctgcgcga
      901 aggcttttgc tgttgccagg ccaagcccgg acgccgcacc cgtcaccagc gcgacctttc
      961 cgtcgaacga gatgttcacc ggtacctcct gtgatcttct gtcgcagcct tcgacgccgc
     1021 taacgctgcg tccgtgcggc gtcggcctcg ggatagcgat caccgatgac ggtgatcttc
     1081 gcggcggcgt cctcgatccg ccgcaactcg tcccgcgtca atacgatcgc cgccgcagcg
     1141 atgttctcct ccagccggtc gagtttggta gtgccgggga tcggaacgat ccatggcttc
     1201 tgcgccagga gccatgccag cgcgatctgc gccggtgtgg cgcccttctg cgccccgatc
     1261 gtttccagca ggtcaaccac cggccggttt gccttgcgag cagcgggcgt aaagcgcggc
     1321 aagcctgtgc gattgtcgtt gccgccgaac gtggtcgtct cgtcgatctt gccggtgaga
     1381 aagcctcttc ccagcgggct aaaggggacg aagcctatcc cgagttcctc gcacgccggc
     1441 agcacatctg cttcgggctg acgccaccag agcgagtatt cgctttggat cgcggcgacc
     1501 ggatgggtcg cgtgcgcgcg gcgaatggtt gcggcgccag cctcggacaa gccgaaatgc
     1561 ctgactttgc cctgcgcgac caagcccttc accgcgccgg cgacgtcctc gatcggcacg
     1621 ccgggatcga cgcggtgctg atagagcagg tcgatcgcct cgacccgcag gctcttcaat
     1681 gatccctcga ccgcctgccg gatgcgctcg ggacgactgt cgagcccgtc agagccgcga
     1741 gcaccggcag gatcgtggcc gaacttggta gcgatcacga cgtccttccg gaacggctcc
     1801 aaggcttccc cgacgagttc ctcgttggtg aacgggccat agacttcggc ggtgtcgaag
     1861 aaggtgacgc cacgctccac cgccgcacgg accagcgcga tcatcgccgc gcggtccggc
     1921 gccggaccgc gatgataatt caagcccatg cagcccaggc cgatggccga gacctcaagg
     1981 cgattcttgc cgagcatgcg ggcctgcata ttcatcctcc cgtgcgcatg ggtgatcggg
     2041 tgctgtcgct caggcggcgg cgtttcactg tgcgccggcc cggtattgcg cgtcgctgac
     2101 atgctccatc caatccacga ccttaccgtt cagcgcttcc tgaagcgcaa tgtgggtcat
     2161 ggccgtggtc ggcgcggcac cgtgccagtg cttctcgttc ggcgcgaaca ggacgacgtc
     2221 gcctggccgg atttcctcga ttgggccgcc gtcgcgctgc acccggccgc agccggcggt
     2281 gacaatcagc gtctgaccca gcggatgggt gtgccacgcc gtccgcgcgc ctggctcgaa
     2341 cgtgacgctc gcaccgcgag cgcgcgccgg gtcgggcgcc tggaacagcg ggtcgatccg
     2401 cac
//
LOCUS       DATGEM010000630         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_610847, whole genome shotgun sequence.
ACCESSION   DATGEM010000630 DATGEM010000000
VERSION     DATGEM010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610847"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VKQ27_19370"
     CDS             complement(<1..687)
                     /locus_tag="VKQ27_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91153.1"
                     /translation="MNDHATLAQRADALEEKMSRELDYIVIKSRLYSLAEGDLNPPPN
                     ANALMAANPNARVLMGDDPRLPPMPEKPTLMDFFKYRFGPSAHLLQSARHAVNAGLPE
                     KMVLACLLHDISTIGFIRGDHGYWGAQLVEPYVDEEVTWAIRAHQVLRFYPDESVGYE
                     YPQSYVKAFGADYRPDQYVEDDYQRMRNHKWYMSGRMITVHDIYSFDPNVHVELEEFT
                     DVIGRNFRQPE"
     gene            complement(744..1334)
                     /locus_tag="VKQ27_19375"
     CDS             complement(744..1334)
                     /locus_tag="VKQ27_19375"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLY91154.1"
                     /translation="MRVLVIYCHPVAESFAAAAHAAVLRVLQASGHEVIDVDLYAEDF
                     DPVMSRQERLDYLNTERNERRVKRYDDQLVGAEALVLVYPAWWYGMPAMLKGYFDRVW
                     LPGVAFDVQSDGTVSTDRLKSLRRIVVITTYGGSWWMVRLAIGDPARKLIGRAVRALC
                     ARDCKVNWLVHYNMDRARPRQLSRFLERIRNAVARL"
     gene            complement(1396..2970)
                     /locus_tag="VKQ27_19380"
     CDS             complement(1396..2970)
                     /locus_tag="VKQ27_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91155.1"
                     /translation="MVGTWKPLGHQPPVAIDTMLLLTDGSVMCHDYGVGGAYTPNWYR
                     LVPDAFMDYANGSWHKLTPMPANAPLSQNGPVDAPLYFASAVLRDGRVFVAGGEYNAS
                     PNVDILACSIYDPAHDSWASIPNPAGWTNIGDAPTCVLPDGRLLMGNINTVQTAILDP
                     ATMTWSAGGDKHDNSSEESWTLLPDGTIIVAEVDNHPRAEKYLIANNQWIQAGQTPPG
                     HDLVLNEPGVSIEIGPAVLMPDGRVFCAGATGHTALYQPPLHPTMPGTWIAGPDFPVS
                     GGHLMRAFDAPASLLPNGSVLVAVGPLNGGWSGPPTVFFEFDGAALHPTPNPASGAGE
                     PTYNARLLLLPSGQVLYANCTSTIEIYTPSGGPMPAWRPQITEVPHHLHPGETYRLHG
                     RQLNGLSQANAYGDDAQMATNYPLVRLQGPPGVGIVFCRTFDHSTMAVATGSAIHHTH
                     FHVPSHLPHGHYELVVIANGIVSDAIAVHVGIEEKREEKHEPHERHEPERHEHREHDD
                     DDGSLELKLKLPLSRR"
     gene            3153..4112
                     /locus_tag="VKQ27_19385"
     CDS             3153..4112
                     /locus_tag="VKQ27_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLY91156.1"
                     /translation="MEDLLASLRAAAEPTRLRLLALAGRGAFCVTEFTEILGQSQPRL
                     SRHLKLLCDCGLLDREREGANVWFTLPHGEAGALARDLVARLPEDDPILEADRRLAAR
                     VLAERARAASESFRRQGADWDEMRALDLPAAAVEEALLRLVPEPDAGRLLDIGTGTGR
                     VLELLAHHISQGIGVDASKAMLALARSRLARADLTHCSVRLADMYRLPLQETSFDIAV
                     LQMVLHYAEDPPGVLAEAARVLRPGGRLLVVDLARHDQDTLLTRYAHRWPGFDDAAMR
                     SLLAEAGFEATEVLDIPGPLAVRIWVGTRAAHAPIAQSRELAV"
     gene            4116..5138
                     /locus_tag="VKQ27_19390"
     CDS             4116..5138
                     /locus_tag="VKQ27_19390"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HLY91157.1"
                     /translation="MELTTFIRGLPKAELHMHLEGSIEAELLLELAARNGLRPRWETP
                     QALRSAYAFSNLQSFLDLYYEGCRVLVQERDFYDMTRAYLRRAHDDAVIRAEVFLGPQ
                     SFIERGVSLAAVMDGTLAAMRDAERDDGISSGLLVTAQRHRSEAEALALLDSVMPWGD
                     RIAGFGLGGAESGNPPSRFANYFRACRERGFRITVHAGEEGPASYVREAVEILDVDRI
                     DHGNSCLDDPALVRVLAERGTPLTVCPLSNVRLKGVPSLEAHPLRSMLAAGLNVTLNS
                     DDPAYFGGYVNDNFIQCEQILCLSADDVVTLARNSLLASFAPPSETASHIARLEGYLA
                     AHQVAS"
     gene            5149..>6152
                     /locus_tag="VKQ27_19395"
     CDS             5149..>6152
                     /locus_tag="VKQ27_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLY91158.1"
                     /translation="MSRPSLLDVLRDRVLLCDGAVGTRVQGMSLDVDLDYHGRENCTD
                     ILNISRPDLVREIHHGYFAAGADMVETNTFGGSPVTLAEFDLADRAFEINQRGAELAR
                     EAAEQFADGRHRWVIGSVGPGTKLPSLGNIAYDPLEAALAEQCRGLIAGGVDAILIET
                     CQDPLQIKAAVNGAKIARAAARSEIPIFVQATVETTGTLLVGPDIAAAAAIVNALDVP
                     MIGLNCATGPQEMAEHIRWLAANWPGMLSVQPNAGLPELVDGQPHYPLGAPELASWLE
                     RFIEEDGVNLIGGCCGTNTQHIAALDAMLRRRGDRRPTPVARKSVWVPSVASMYQAVP
                     L"
BASE COUNT         1059 a         1930 c         2067 g         1096 t
ORIGIN      
        1 ttccggctgg cggaagttgc ggccgatcac atcggtgaat tcctccagct cgacatgcac
       61 attcggatcg aacgaataga tgtcgtgaac tgtgatcatg cggccggaca tgtaccattt
      121 gtggttgcgc atccgctggt aatcgtcctc gacatactga tcaggccggt aatcggcgcc
      181 gaacgctttg acgtaagact gcggatactc gtagccgaca gattcatcgg ggtagaagcg
      241 gagcacctga tgcgcgcgga ttgcccaggt gacttcctca tcgacatagg gctcgacgag
      301 ctgtgcaccc cagtagccgt gatcgccgcg gatgaagccg atggtcgaga tatcgtgcag
      361 cagacaggcg agcaccatct tctccggaag gccggcgttc accgcgtgcc gcgcgctttg
      421 cagaagatgc gccgaagggc cgaaacggta tttgaagaag tccatcaacg tgggcttctc
      481 cggcatcggc ggcaggcgag gatcgtcgcc catcaggacg cgcgcgttcg gattggcggc
      541 catcagcgca ttggcgttgg gcggcgggtt caggtcgcct tcggcaaggc tgtagaggcg
      601 cgacttaatc acgatgtagt caagttcacg actcattttc tcttcgagcg cgtcggcgcg
      661 ttgcgcgagg gttgcgtggt cgttcatggc gcttctccct ggagcgagca gaaaggctag
      721 cagggcaagg cggatggcgt cgactacagc cgggcaacgg cgttgcggat gcgttcgagg
      781 aaacgcgaca gctgccgcgg ccgggcacgg tccatgttgt agtgaaccaa ccagttgacc
      841 ttgcaatcgc gggcgcaaag ggcgcgcacc gcgcgtccaa tcagcttgcg ggccggatcg
      901 ccaatcgcga gacgcaccat ccaccatgag ccgccatacg tcgtgatcac gacgatgcgg
      961 cggagcgact tcagccggtc ggtgctgacc gtgccgtcgg actgcacgtc gaacgcaacg
     1021 cccggcagcc agacgcgatc gaagtagcct ttcagcatgg cgggcatgcc gtaccaccag
     1081 gcggggtaga ccagcaccaa agcctcggca ccaacaagct gatcgtcgta gcgcttcacc
     1141 ctgcgttcgt tgcgctccgt gttcagataa tccaggcgtt cctgccgact catgaccgga
     1201 tcgaaatctt ccgcatagag atcgacgtcg atcacctcgt gcccggaagc ttgcagcacc
     1261 cgaagcacag cagcgtgagc cgcggcagcg aagctttcag ccactgggtg gcaatagatg
     1321 acaagaacgc gcatgcctgc gatctggggg cgaaacagcc ccggatcgca ggcacccgcg
     1381 cagtcgtgat tgcggctaac gccggctgag cgggagcttc agcttcagtt cgaggctgcc
     1441 gtcgtcatcg tcatgctcgc gatgttcatg gcgctccggt tcatgccgct cgtgcggttc
     1501 gtgcttttct tctctcttct cctcgatccc cacgtgcacc gcgatggcat ccgagacgat
     1561 accgttggcg atcaccacca gttcatagtg accgtgcggc agatgcgacg ggacatggaa
     1621 atgtgtgtga tggatggcgc tgccggttgc cacggccatc gtcgaatgat cgaacgtgcg
     1681 gcagaacacg atgccgacgc caggcggacc ttgcaggcga acaagcggat agttggtggc
     1741 catctgggcg tcgtcgccgt aggcattggc ctgcgagagg ccattcaatt gccgcccatg
     1801 cagccggtag gtttcgcccg gatgcaggtg atgtggcact tcggtgatct gcggccgcca
     1861 tgccggcatc gggccgccgg acggggtata gatctcgatc gtcgaggtgc aattggcata
     1921 tagcacctgc ccgcttggca gcagcagcag acgcgcgttg tacgttggtt ccccggcgcc
     1981 gctggcgggg tttggcgtcg gatgcaaggc agcgccgtcg aactcgaaga acaccgtcgg
     2041 cggaccagac cagccaccgt tcagcggacc aaccgcgacc agcaccgagc cgttcggcag
     2101 cagggatgcc ggcgcatcga acgcgcgcat cagatggcca ccagataccg ggaaatccgg
     2161 gccggcgatc cacgtgccgg gcatcgtggg atgcagcggc ggctgataca gcgcggtgtg
     2221 gccggtagcg ccggcgcaga acacgcggcc gtccggcatc agcaccgccg gcccgatctc
     2281 gatcgagacg ccgggttcgt tcagcaccag gtcgtggccg ggcggcgttt gtccggcctg
     2341 gatccactgg ttgttggcga tcaggtattt ctcggcgcgg gggtggttat cgacctcggc
     2401 cacgatgatc gtgccgtctg gcagcagggt ccagctttcc tcggaactgt tgtcgtgctt
     2461 gtcgccgcct gcgctccagg tcatcgtcgc cggatcgagg atcgcggtct gcacggtgtt
     2521 gatgttgccc atcagcaggc gcccgtccgg cagcacgcag gtcggagcat cgccgatgtt
     2581 ggtccagccc gccgggtttg gaatgctggc ccaggaatcg tgcgcaggat cgtagatgga
     2641 gcaggccaaa atatcgacgt tcgggctggc attgtattca ccgccggcga cgaacacgcg
     2701 cccgtcgcgc agcacggccg aggcgaaata gagcggcgca tcaaccggtc cgttctgcga
     2761 caacggcgcg ttggccggca tcggtgtcag tttgtgccaa ctcccattgg cgtaatccat
     2821 gaacgcatcc ggcacgagcc ggtaccagtt tggcgtgtag gccccaccga cgccgtaatc
     2881 gtggcacatc acgctgccat cggtgagcag cagcatggtg tcgatggcga cgggcggctg
     2941 atgcccgaga ggcttccaag ttccgaccac gtgtttcccc tttctccccg caactgtgat
     3001 ggcgggtgca gtgcgatgga tgcgcccggg cgagccttcc cgggtgtgac gtggttcatt
     3061 accggaaccg cacatttgct tgagcgcgga cggcgagacg agcgcgaccg gcgaacctgg
     3121 acataaggat atgtttatgt cacagatggc ttatggagga tctgcttgcc agcctgcgcg
     3181 ccgccgccga accaacccgc ctgcgcctgc tggcgctcgc tggccggggc gcgttctgcg
     3241 tcaccgagtt caccgaaata cttggccagt cacagccccg cctgtcccgc cacctgaaac
     3301 tgctgtgcga ctgcgggctg ctcgatcggg agcgagaggg ggcgaacgtc tggtttaccc
     3361 tgccgcacgg cgaagcgggt gccttggcgc gggatctggt cgcccggctg ccggaggatg
     3421 acccaatcct ggaggcagat cgccggctcg ctgcccgggt gctggccgaa cgcgcccgcg
     3481 cagcctccga gagtttccgg cgccagggcg ccgactggga cgagatgcgc gcgctggacc
     3541 tgccggccgc ggccgtggaa gaggcactgt tgcgccttgt gccggaaccg gacgccggcc
     3601 ggctgctcga catcggcacc ggcaccgggc gcgtgctgga actgctcgcc caccacatca
     3661 gccagggcat cggggtggat gcgtccaagg ccatgctggc gctggctcgt tcgcgtctcg
     3721 cgcgcgccga cctgacgcat tgctcggtgc gcctcgcgga catgtatcgc ctgccgctgc
     3781 aggaaacctc gttcgacatc gccgtgctgc aaatggtgct gcactacgct gaagatccgc
     3841 cgggcgttct cgccgaggct gcgcgcgtac tgcgccctgg cggccgcctg ctcgtggtgg
     3901 acctggccag gcacgaccag gacaccctac tgacgcggta cgcgcaccgc tggccgggct
     3961 tcgacgacgc ggcgatgcgc tcgctgctgg ccgaagccgg cttcgaagcg accgaggtgc
     4021 tggatattcc ggggccactt gcagtgcgca tctgggtcgg cacacgcgca gcgcacgcgc
     4081 ccatcgcgca gtcgcgcgaa ctggcagttt aggcaatgga gttaacaacc ttcatccgtg
     4141 ggctgcccaa ggcggaattg catatgcatc tcgagggcag catcgaagcg gagctgctgc
     4201 ttgaactggc cgctcgcaac ggacttcggc cgcgctggga gacaccgcag gctctgcgct
     4261 ccgcctacgc gttcagcaac ctgcaatcgt ttctggatct ttactacgag ggttgccggg
     4321 tcctggtgca ggaacgggac ttctacgaca tgacccgcgc ctaccttcgc cgcgcgcacg
     4381 acgacgcggt gatccgtgcg gaagtgttcc tgggcccaca gagcttcatc gagcgcggtg
     4441 tttcgctggc ggcggtcatg gacggcacgc ttgcggcgat gcgagacgcc gaacgggacg
     4501 acggcatcag cagtggcctg ctggtgaccg cgcagcgcca tcgtagcgaa gccgaggcgc
     4561 ttgcattgct cgatagcgtc atgccctggg gggatcggat cgctggcttc ggcctgggcg
     4621 gcgcggagag cggcaatccg ccatcgcggt tcgccaatta cttccgtgcc tgtcgcgagc
     4681 gtggcttccg catcaccgtg catgcgggcg aagaggggcc ggcgtcctat gtgcgcgagg
     4741 ccgtggagat cctggatgtg gaccgcatcg accatggcaa ttcctgcctc gacgacccgg
     4801 cgctggtgcg cgtgctggcg gaacgaggga cgccgctgac agtatgcccg ctatcgaatg
     4861 tccggctgaa aggcgtgccg tcgcttgagg cacacccact gcggagcatg ctggcggcgg
     4921 gactgaatgt gacgctgaac tcggacgatc ccgcgtattt cggcggctat gtgaacgaca
     4981 atttcattca atgcgagcag atactgtgcc tgtcggcaga cgacgtcgtc acgctggcgc
     5041 gcaacagcct gcttgcctcc tttgctccgc ctagcgagac ggcgagccac attgcgcggc
     5101 tcgaaggcta tctcgctgcc caccaggtcg cttcctgaag gaacaccgat gtcccgtccc
     5161 tccttgctcg atgtgctgcg cgatcgtgtg ttgctgtgtg acggcgctgt ggggacgcgc
     5221 gtgcagggaa tgtcgttgga cgtcgatctg gattaccacg ggcgggagaa ctgcaccgat
     5281 atcctgaaca tttcccgccc ggatctggtg cgggagatcc atcacggcta cttcgccgcc
     5341 ggcgccgata tggtggagac caacacgttc ggcggctcgc cggtgacgct ggcggaattc
     5401 gacctggcgg accgcgcctt cgagatcaac cagcgcggcg ccgaactggc gcgcgaagct
     5461 gccgagcagt ttgccgatgg ccggcatcgc tgggttatcg gcagcgtcgg gccgggcacc
     5521 aagctgccga gcctgggcaa catcgcgtat gacccgctgg aggccgcgct cgctgagcaa
     5581 tgccgtggcc tgattgccgg tggcgtcgat gcgatcctga tcgagacgtg ccaggacccg
     5641 ctgcagatca aggcggcggt gaatggtgcc aagatcgctc gcgccgccgc ccgcagcgaa
     5701 atcccgatat tcgtgcaggc gactgtggag acgacaggca ctctgctggt cggaccggac
     5761 atcgccgccg cggcagcgat tgtgaacgcg ctcgacgtgc cgatgatcgg gctgaactgc
     5821 gcaaccggcc cacaggagat ggcggagcac atcaggtggc tcgcggccaa ctggccggga
     5881 atgctgtcgg tgcaaccgaa tgccggattg ccggaactgg tcgatggcca gccgcactac
     5941 ccgctcggcg ctcccgaact ggcgagctgg ctggagcgat tcatcgagga ggacggcgtc
     6001 aacctcatcg gcggctgctg cggcaccaac acccagcaca tcgcagcact cgatgcgatg
     6061 ctgcggcggc gcggcgaccg tcggcccaca ccggtggcgc gcaaatccgt gtgggtgccg
     6121 tcggtcgcga gcatgtacca ggccgtgccg ct
//
LOCUS       DATGEM010000631         6096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1567847, whole genome shotgun sequence.
ACCESSION   DATGEM010000631 DATGEM010000000
VERSION     DATGEM010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567847"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKQ27_19400"
     CDS             <1..476
                     /locus_tag="VKQ27_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HLY91159.1"
                     /translation="DQRQCHILGADGRIAQHTGANCVVWAGHARGPDVSVAGNMLAGP
                     QVVQRTLEAYLGSGGSLAERLIYALEAGEAAGGDKRGKQSAALKICTRDPYPDLDIRA
                     DDHPDPLAELRRLYRVSLERFAIFRRHVPGRDNPSGTLDRSIIDADITRGRVAPV"
     gene            507..2015
                     /locus_tag="VKQ27_19405"
     CDS             507..2015
                     /locus_tag="VKQ27_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY91160.1"
                     /translation="MRRFAFGLFCALWLVAPAMAQERILHVGLREDPDLLDPTLGSSY
                     VGRIVFAGMCDKLFDIDAKLNIVPQLATGYEWKDPTHLVIHIRDGVQFQDGEKLNADA
                     VKYKLMRDLTIKGSMRRGEVSSIDSIDVIDPLTVQLNLKAPASQLLAQLTDRAGIMIS
                     PKAAEAAGDKFGLHPVCAGPFSFESRVAQDRIVVKRFPGYWDAKDIHFDQVVYLPNPN
                     SSVRLANLQAGALDLVEYILPTDVAAVQKDPKLKVAIDDSLAYNGITFNTGNGTAQDS
                     PAGKNALVRQAFELGIDRQALIQVVHNGMFTPTAQANPPSSPFYIKDVQPPARDPTKA
                     KALLQQAGVTMPVPVTLTITNGSDTQQVGEVIQSMLSEAGFDVKIKTMEFASSLQAGY
                     AGDFQAYMIGWSGRADPDGNMWQMLHTGGQFNYGHYSNPEVDKALDDARTVAGVDQRR
                     AFYTKVWEQERKDLPLVYLWTAKNIVGMKKSITGFQQVPDGLIRLQGMEMGK"
     gene            2118..2315
                     /locus_tag="VKQ27_19410"
     CDS             2118..2315
                     /locus_tag="VKQ27_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91161.1"
                     /translation="MIDSEVRPGERAPATGSYEELNVFGVRTGKVLHVRTGEALPAAP
                     RGFSWRLAQRSASSGPGWGSA"
     gene            complement(2349..3167)
                     /locus_tag="VKQ27_19415"
     CDS             complement(2349..3167)
                     /locus_tag="VKQ27_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91162.1"
                     /translation="MANAAPEIPVAFPSDPPQDHCDHAVPGNIGVLLHAVRTLLTYGR
                     HLIDTVRQRSTAPGFNAIAACFGTANLSTILAHLNRGILRCIALERVLLTRAATGKDI
                     DYIARRSSLTMEEYLARLDAAQPEQPATAAPTTRTSAPGPSRPAGWNDPELFMPTLED
                     LERQMRRQPIGRIILDISLDLAVVPGFCHSQFWNELFNIMNWFGGGNSIDRLMREKTR
                     RREAFASEQDRDPKANWDWIRPSRDALRQILGFFIGEPPVNPLDPAAAIATAPP"
     gene            3298..3834
                     /locus_tag="VKQ27_19420"
     CDS             3298..3834
                     /locus_tag="VKQ27_19420"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLY91163.1"
                     /translation="MATLVFGLNQSLDGYVDHDHQEFVPGRSLFRHFTEQVRDLAGSI
                     YGRRMYEVMRYWDEDRPEWSAEDRDFAAAWRSQPKWVASRSLKSVGPNATLVADDIEA
                     VVRGLKARLVGEIAVGGPDLARSLTDLGLIDAYRLYFRPVVLGRGKPFFAGPRPPLRL
                     VASDLIGEDAIRLTYVPA"
     gene            complement(3918..5573)
                     /locus_tag="VKQ27_19425"
     CDS             complement(3918..5573)
                     /locus_tag="VKQ27_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HLY91164.1"
                     /translation="MAPPAAPPQQRRFRARWVSPLLRRILLVNALPLALLVAALLYLD
                     QYQNGLLEAEVSALREQARIYAGALGESAVRISEPDNPKLVADIARPLLHRLTDPAPG
                     AQAKLYAPDGTMIADSRVREGPSGAVSTEPLPPAEEHGPIVGAVGWLYDKVMAVLPHE
                     TPSMVAVDMTPGAGGLEWQPNVKEELSVSSSGQSREMPPYIRRTKDDQLLVTVAEPVE
                     RDHHTVGVILLTRDAREVDSSLFIVRISILALFALALALTVLLSWYLSLTIARPILRL
                     AGVAAELREGHGRAGVMPAPLLQRHDEIGMLATALADSTQALFTRMDAIERFAADVAH
                     EIKNPLSSIRSAIETLRRVQDPDRQRQLLTIIQQDVTRLDRLISDVSDASRLDAELSR
                     VTAERVDIVPILQTLRELDEATRDSENDPALVVDAPASLLVWAIEDRLVQVLRNLIAN
                     AQSFSPPRGRITLTAHDAGAMVEISVEDEGPGIPETNLEQVFDRFYSERPQGEKFGQH
                     SGLGLSISRQIVEALRGQILAENRKGIGNRVLGARFVVRLPKA"
     gene            complement(5602..>6096)
                     /locus_tag="VKQ27_19430"
     CDS             complement(5602..>6096)
                     /locus_tag="VKQ27_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLY91165.1"
                     /translation="SRSSLPVIFLTSKDEEVDELMGLRLGADDYITKPFSQRLLIERI
                     RALLRRNEAGRAEGSGAAPGGVMVRGELTLDETKHQCIWQSQDIQLTVTEFLLVKALA
                     ARPGMVKSRDQLIDAAYGENIYVDDRTIDSHVKRIRKKFRTVDDNFDHIETLYGIGYR
                     YKEH"
BASE COUNT         1020 a         1742 c         2213 g         1121 t
ORIGIN      
        1 cggatcagcg gcagtgccat atccttggag ccgatgggcg gatcgcgcag cacacaggtg
       61 cgaactgcgt ggtctgggcg ggacatgcgc gcgggcctga cgtgtcggtc gccggcaata
      121 tgctggccgg gccgcaggtc gtgcagcgga ccctggaggc gtatctcggg tcgggtggca
      181 gtctggcgga gcggctgatc tacgcgctgg aggccggcga ggccgcggga ggcgataagc
      241 gcggcaagca gtcggcggcg ctgaagatct gcacgcgcga tccgtatcct gatctggata
      301 tccgcgcgga cgatcatcct gatccgctgg cggagctgcg gcggctgtac cgggtcagcc
      361 tggagcgctt tgcgatcttc cggcggcatg tgcctggccg ggacaatccg tcgggcacgc
      421 tggatcgcag catcatcgac gccgatatca cgcgaggccg ggtggcaccg gtttagagca
      481 agatgcgcga tcagggaggg aaaaggatgc gccgctttgc gtttggtctg ttctgtgcgc
      541 tgtggctggt tgcgccggcg atggcgcagg agcgcatcct gcatgttggt ctgcgcgagg
      601 atcccgatct gctcgatccg acgctggggt cctcgtatgt cggacgcatc gtgttcgcgg
      661 gcatgtgcga caagttgttc gatatcgatg cgaagctgaa catcgtgccg cagcttgcga
      721 cagggtacga gtggaaagac ccgacgcatt tggtgatcca tatccgcgat ggcgtgcagt
      781 tccaggatgg cgagaagctg aacgcggacg cggtgaagta caagctgatg cgcgacctga
      841 cgataaaggg cagcatgcgg cgcggcgagg taagctcgat cgacagcatc gacgtgatcg
      901 atccgctgac ggtgcagctc aacctgaagg caccggcatc gcagttgctg gcacagctga
      961 ccgaccgcgc gggtattatg atctcgccga aggcggccga ggccgcgggc gacaagttcg
     1021 ggctgcatcc ggtgtgcgcc gggccgttca gcttcgagag ccgcgtggcg caggatcgca
     1081 tcgtggtgaa gcggtttcct ggctactggg atgcgaagga catccatttc gaccaggttg
     1141 tgtatttgcc taatccgaac tcgtcggtgc ggctggcgaa tttgcaggcc ggagcgctgg
     1201 acctggtgga atacatcctg ccgacagatg tggccgcagt gcagaaggat ccgaagctga
     1261 aggtggcgat cgacgattcg cttgcctaca acgggattac cttcaacact ggcaatggga
     1321 cggcgcagga ttcgccagcc ggcaagaacg cgttggtgcg gcaggcattc gagttgggaa
     1381 tagatcggca ggcgctgatc caggtggtcc acaacggcat gttcacgccg acggcgcagg
     1441 ccaatccgcc gagctcaccg ttctatatca aggacgttca gccaccggcg cgcgatccga
     1501 ccaaggcgaa ggcactgctg cagcaggctg gcgtgacgat gccggtgccg gtgactttga
     1561 ccatcacgaa cgggtcggac acgcagcagg tgggcgaggt gatccagtcg atgctgagcg
     1621 aggccgggtt cgacgtgaag atcaagacga tggagttcgc ttcctcgttg caggcgggat
     1681 acgcgggtga tttccaggcc tacatgatcg gctggtcggg ccgagccgat ccggacggca
     1741 atatgtggca gatgctgcac acgggcgggc agttcaacta cgggcactat tccaatcccg
     1801 aggtggacaa ggcgctggat gatgcgcgga cggtggcggg ggtggaccag cggcgtgcgt
     1861 tctataccaa ggtctgggag caggagcgga aggacctgcc gctggtatat ctgtggacag
     1921 cgaagaatat cgtggggatg aagaagagca tcaccggctt ccagcaggtt cctgatgggc
     1981 tgatccggct gcaggggatg gagatgggaa agtaggcggg cccgggcgcg gtccgtacgg
     2041 acaatccacg ataggaaagg ccggcccacg agcctatgtc tgcgcgagat atgacccatt
     2101 ctagtgttgt ggcaggcatg attgacagcg aagttcggcc gggcgagcgc gcgccggcaa
     2161 ccggttctta cgaggagctg aacgttttcg gcgtgcgcac gggcaaggtg ctgcatgtcc
     2221 ggacgggcga agcattgccg gccgcgcctc gcggcttttc gtggcggctc gcgcagcgtt
     2281 cggcatcgag cgggcccggc tggggctctg cgtagttccg gtgcccgacg cgggagttgg
     2341 gtggcgggtc agggcggggc ggtggcgatc gcggcggcgg ggtcgagcgg gttgaccggc
     2401 ggttcgccga tgaagaagcc gaggatttgg cgcagcgcgt cgcggctcgg acgtatccag
     2461 tcccagttgg ctttcggatc gcggtcctgc tcgctggcga atgcctcgcg gcggcgggtt
     2521 ttctcgcgca tcagccggtc gatgctgttg ccgccgccga accagttcat gatgttgaac
     2581 agctcgttcc agaattggct gtggcagaag ccagggacga cggcgagatc gaggctgata
     2641 tcgaggatga tgcggccgat gggctggcgg cgcatctgac gctcgaggtc ctccagggtt
     2701 ggcatgaaga gttcgggatc gttccagccc gcggggcggg aggggcctgg cgcgctcgtg
     2761 cgggtggtgg gggccgcggt cgctggttgc tcgggctgcg cggcatccag gcgggcgagg
     2821 tattcctcca tcgtgaggga ggagcggcgc gcgatgtagt cgatgtcttt gccggtggcg
     2881 gcgcgggtga ggaggacgcg ctcgagcgcg atgcagcgca ggatgcctcg attgagatgg
     2941 gccaggatgg tggaaagatt ggcggtgccg aagcaggcgg cgatggcgtt gaagccgggc
     3001 gcggtggagc gctggcggac ggtgtcgatc aggtggcggc cgtaagtgag cagggtgcgg
     3061 acagcgtgca gcaggacccc gatgttgccg gggacggcat ggtcgcagtg atcctgcggg
     3121 ggatctgaag ggaaggcgac gggtatctcg ggcgctgcgt tggccatgtt tggtgtgcta
     3181 ccagagaacg ttagtaaagt caacgatttt gttcccatcg attctggggc tggtctctca
     3241 ctgacgcgtc acggcacccg ggctattctg atgcacctgg gatggagatt ttgcagcatg
     3301 gcgacgctcg tcttcggctt gaaccagtcg ctggacggct acgtcgacca cgaccaccag
     3361 gaattcgtgc ctggtcgctc gctgtttcgt cacttcaccg agcaagtacg tgatctggct
     3421 ggcagtatat acgggcgccg catgtacgag gtcatgcgtt actgggacga agaccgtcct
     3481 gagtggagcg ccgaggatcg cgacttcgcg gcggcgtggc ggagccagcc gaagtgggtc
     3541 gcgtcgcgct cattgaagtc agtcggccct aacgccacgc tggtcgcgga tgacatcgag
     3601 gcggtggtgc gtgggctgaa ggctcggctt gttggcgaga tagcggttgg cggaccggac
     3661 ctggcgcgaa gcctgaccga ccttggtctt atcgatgcgt atcgactcta tttccgccct
     3721 gtcgtgcttg gtcgcggcaa gccgttcttc gctggtcccc ggccgccgct tcgccttgtg
     3781 gcgagcgatc tgatcgggga ggacgcgatc aggctgacat acgtgccggc ctgagcgcgc
     3841 gaattgcacg gtgtgagagt gggctccgga gggctcggag cccatcactg ccacgcgtgc
     3901 cgggatcact gctaccgcta tgctttcggc agccgcacca cgaagcgggc accgagcacg
     3961 cggttgccga tgcctttgcg attctcggcc aggatctgcc cgcgcaatgc ctcaacgatc
     4021 tggcggctga tcgacaggcc gaggccggaa tgctggccga atttttcgcc ctgcggacgc
     4081 tcggaataga aacggtcgaa tacctgttca aggttcgttt cgggaatgcc cggcccttca
     4141 tcctcgacgc tgatttccac catggcgccc gcgtcgtgtg cggtcagcgt gatgcggcca
     4201 cggggtgggc tgaatgactg ggcattggcg atcaggttgc gcagcacctg caccagccgg
     4261 tcctcgatag cccagaccaa caggctggcc ggtgcgtcga ccacaagcgc ggggtcgttt
     4321 tcggaatcgc gggttgcctc gtccagctcg cgcagtgttt gcaggatcgg cacgatatcg
     4381 acccgctcgg cggttacgcg ggacagttcc gcgtccagcc gcgaggcgtc gctgacatcg
     4441 ctgatcagcc ggtcgagccg ggtcacatcc tgctgaatga tggtcagcaa ctggcgctgg
     4501 cggtcgggat cctggacgcg gcgcagcgtt tcgatggcgc tgcggatcga cgacagcggg
     4561 ttcttgatct catgcgcgac atcggcggca aaccgctcga ttgcgtccat gcgggtgaac
     4621 agcgcctgcg tcgagtcagc cagggcggtg gcgagcatgc cgatctcgtc gtgccgctgc
     4681 agcagcgggg ctggcattac gccggcgcgg ccgtggccct cgcgcaattc agccgccacg
     4741 ccggcgagcc ggaggatggg ccgcgcgatg gtgagcgaca ggtaccagga cagcagcact
     4801 gtcagtgcca gcgccagcgc gaacagtgcc aggatcgaga tgcgcacgat gaacaggctg
     4861 gaatcgactt cacgcgcgtc gcgggtcagc aggatgacgc cgacggtgtg atgatcgcgc
     4921 tccaccggtt ccgccacggt gaccagcaac tggtcatcct tggtgcgccg gatatacggc
     4981 ggcatttccc ggctctggcc agagctgctg acgctgagct cttccttcac gttcggttgc
     5041 cactccaggc cgccggcgcc gggggtcatg tccaccgcca ccatgctcgg cgtctcgtgc
     5101 ggcaggactg ccatcacctt gtcgtagagc cagccgacgg cgcccacgat cggcccgtgc
     5161 tcctcggccg gcggcagggg ctcggtgctg acggcgccgc tgggaccttc gcgcacccgg
     5221 gagtcggcga tcatcgtgcc gtcgggtgcg tagagcttgg cctgcgcgcc aggggccgga
     5281 tcggtcagcc gatgcagcag tgggcgtgcg atgtccgcca ccagcttcgg attgtcaggc
     5341 tcgctgatgc gcaccgcgct ttcacccagc gcgccggcgt agatgcgcgc ctgttcgcgc
     5401 aatgcggaga cctcggcttc caggaggcca ttctgatact gatcgagata cagcaatgcc
     5461 gccaccagca gtgccagagg cagcgcgttc accagcagga tgcggcggag cagcggcgac
     5521 acccagcgag cgcggaagcg gcgctgctgc gggggggcag caggcggagc cagcgctgta
     5581 agggtgatgc cgtcgggcag tttagtgctc cttgtaccga tagccgatgc cgtagagcgt
     5641 ctcgatatga tcgaagttgt cgtccactgt acggaacttc ttgcggatac gcttgacgtg
     5701 gctatcgata gtccggtcat cgacatagat gttctcgcca tatgccgcgt cgatgagctg
     5761 gtcacggctc tttaccatgc caggccgcgc cgcgagcgcc tttaccaaca gaaattcggt
     5821 gacggtcagc tggatgtcct ggctctgcca gatgcactgg tgcttggtct catccagggt
     5881 gagttcgccg cgcaccatca ccccgccggg cgcggcgccg gagccttccg cccgtccggc
     5941 ctcgttgcgg cggagcaggg cgcggatgcg ctcgatcagc aggcgctggc tgaacggctt
     6001 ggtgatgtag tcgtcggcgc cgaggcggag gcccatgagt tcgtccacct cctcgtcttt
     6061 cgaggtgagg aagatgacgg ggagggagga gcggct
//
LOCUS       DATGEM010000632         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_703524, whole genome shotgun sequence.
ACCESSION   DATGEM010000632 DATGEM010000000
VERSION     DATGEM010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703524"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VKQ27_19435"
     CDS             complement(<1..1293)
                     /locus_tag="VKQ27_19435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLY91166.1"
                     /translation="MEPWALSLFVSVIHLVLALTVSAHIVLTKPDVRASIGWVGLVWL
                     APVVGSALYAMFGINRIRRQAGRIRRGRELLWTQAPGPFVLSSDVAGLPQGVAPAARA
                     LATLVGAVTKLPLMAGNEVDMLENGDEAYPAMLSAIDGAQVSVALATYIFNRGHVADQ
                     FVDALERAQRRGVAVRVLIDGVGARYSRPPIVRTLRAHGITVARFLPPSMPWRHPYFN
                     LRNHRKLMVVDGMTGFCGGLNIQDGCMLSLDTDEPIRDLHFRIRGPVVGQLMEALAFD
                     WTFTTKEELAGPAWFPRIEPVGDVHARGIADGPDEDNEVLLVTILGALSQATQSVRIV
                     TPYFLPDGTLIDALRVAALRGVQVDILLPARGNLRFVDWAAAAQLAQIVRWGCRVHLS
                     APPFDHSKLFLVDDAWCMIGSANWDPRSLRLNFEYAVEC"
     gene            complement(1321..1959)
                     /locus_tag="VKQ27_19440"
     CDS             complement(1321..1959)
                     /locus_tag="VKQ27_19440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLY91167.1"
                     /translation="MTAKTKSAGDAVAPKERVKRQEPRRLDRAAFNRPTLQVARDLLG
                     KFIVRRYRSRHICAMITEVEAYKGPRDKAAHSYNGRRTRRVEPLYDDGGTVYVYFIYG
                     IHWMLNFSTAGEDMPEGVLIRGILADLDGEEKLLAGPGRVTKYLRIDKKLDGADVTDS
                     AQIWLEDRGVRIPARFIKRGPRIGVDFAGPFWAARPWRFWIERSTAALVAHP"
     gene            complement(2237..3592)
                     /locus_tag="VKQ27_19445"
     CDS             complement(2237..3592)
                     /locus_tag="VKQ27_19445"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HLY91168.1"
                     /translation="MSGLLDALFRADAVGEIFSDRKRLQGMLDFEAALARAEARVGVI
                     PVNAVPVIEAQCRAELFDVEALARATVLAGNPAIPTVKALTALVAKKDAQVAGYVHWG
                     ATSQDAMDTGLVLQLRAAFDLFDADLAQLAGALARLAIKHKRTPMAGRTWLQQAVPVT
                     FGLKAAGALSAVERHRARLRELRARVLVIQFGGAAGTLASLGGRGLDVAKALATELQL
                     GEPNLPWHAHRDRMAEVATTIGLLTGTLGKIAKDMSLLMQTEMAEAFEPAAPGKGGSS
                     TMPHKRNPVGAAVVLAAATKVPALVSVMLAAMVQEHERGLGNWHAEWETLPEICMLCA
                     GALAQTVQMIEGMEIDTARMRHNLDATHGLILAEAVSAALAPKIGRDTSHQLIESACR
                     RAIEQQKPLRDVLAKDTKVSKLLSPSDLDRLLDPANYLGVAGQFIDRVLASRTGRKSK
                     T"
     gene            complement(3594..4154)
                     /gene="pcaG"
                     /locus_tag="VKQ27_19450"
     CDS             complement(3594..4154)
                     /gene="pcaG"
                     /locus_tag="VKQ27_19450"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HLY91169.1"
                     /translation="MNLPATASQTVGPFFRIGLEALYVSDLAPAAPAADKIAFQGRVL
                     DGDGKPVNDAILEIWQANAHGKYAHPDDAQDKPVTPDFRGFGRVATDDNGVFRFITIK
                     PGSVAGPHGMPQAPHLLVAVFMRGLLIHLLTRIYFPGETANADDPILALVHADRRQTL
                     VARKTADGLEWDVILQGDHETVFFSY"
     gene            complement(4151..>4638)
                     /locus_tag="VKQ27_19455"
     CDS             complement(4151..>4638)
                     /locus_tag="VKQ27_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HLY91170.1"
                     /translation="IVGGRVLDENKRAVPNALVEIWQCNAAGRYRHQNDNHDAPLDPN
                     FSGAGRVLTDEDGRYRFVTIKPGAYPWRNHHNAWRPAHIHLSLFGRAFVTRLVTQMYF
                     PGDPLLPHDPIFNGTANEGARHRLVADFDWDITVPEHALGYRFDIVLRGRDATPMEKT
                     R"
BASE COUNT          848 a         1491 c         1463 g          836 t
ORIGIN      
        1 gcattcgaca gcgtattcga aattcaggcg caggctgcgc ggatcccagt tcgccgagcc
       61 gatcatgcac catgcgtcgt cgaccaggaa cagtttcgag tggtcgaatg gcggcgcaga
      121 cagatgcacg cggcatcccc agcgcacaat ctgcgccagt tgggcggcgg cggcccaatc
      181 gacaaagcgc aggttcccgc gcgccggcag caggatatcg acctgcacgc cgcgcaaggc
      241 ggcgacacgc agcgcgtcga tcagcgtccc gtccggcagg aagtaaggcg tgacaatccg
      301 gacggactgc gtcgcttgcg agagcgcgcc cagtatggtc acgagcagca cctcgttgtc
      361 ttcatccgga ccatcggcga tgccgcgggc atgcacgtca ccaacaggct cgatccgagg
      421 aaaccaggcg ggcccggcga gttcctcttt ggtcgtgaag gtccagtcga acgcgagcgc
      481 ctccatcaac tggccgacca ccggcccacg gatgcgaaag tgcaggtctc gaatcggctc
      541 atcggtgtcc agcgagagca tgcacccgtc ctgaatgttg aggcccccgc aaaagccggt
      601 catcccatcc accaccatca gcttgcgatg gttgcgcagg ttgaaatagg gatggcgcca
      661 cggcatggac ggaggcagga agcgcgcgac cgttatcccg tgcgcgcgca aagtccgcac
      721 gatcggcggg cgactgtagc gcgcgcccac gccgtcgatc aggacgcgca cggcaacccc
      781 tcgccgttgt gcgcgttcga gggcgtcgac gaactggtcg gcaacatgcc ctcgattgaa
      841 gatatacgtc gccaacgcga cgctgacctg cgcgccatcg attgccgaaa gcatggccgg
      901 ataggcttcg tcgccatttt cgagcatgtc cacttcgttg ccggccatca gcggcaactt
      961 ggtgacagcg cccaccaggg tggcaagggc acgagcggcc ggcgcaacac cctgcggcaa
     1021 cccggccacg tcggacgaca gcacgaacgg accaggagcc tgcgtccaca gcaactctct
     1081 gccgcggcgt atgcgcccgg cctggcggcg aatacggttg atgccgaaca ttgcatagag
     1141 cgctgacccg acgaccgggg cgagccatac cagtcctacc cagccaatcg acgcacgcac
     1201 gtccggcttg gtgagaacga tgtgggcgga cacggtcagc gcaagaacca ggtggatcac
     1261 gctgacgaag aggctcagtg cccagggctc catttgcatc acatccaaac cctaacagta
     1321 ttagggatgg gcgaccagtg cggctgtgct gcgttcgatc cagaatcgcc aaggccgcgc
     1381 agcccagaac ggccccgcga agtcgacgcc gatgcggggt ccgcgcttga tgaatcgcgc
     1441 cgggatgcgg actccgcggt cctcgagcca gatctgcgcc gagtcggtaa catccgcgcc
     1501 atcgagcttc ttgtcgatcc tcagatattt ggtgaccctg cccggtccgg cgaggagttt
     1561 ttcctcgccg tcgaggtcgg ccaggatgcc gcgaatcaga acgccctctg gcatgtcttc
     1621 accagcggta ctgaagttga gcatccagtg gatgccgtag atgaagtaca cgtacacggt
     1681 cccgccatcg tcgtagagcg gttcgactct ccgagtgcgg cgcccgttgt atgagtgcgc
     1741 cgctttgtct ctcggtccct tgtacgcctc cacttcggta atcatcgcgc agatatggcg
     1801 gctccggtac ctccgcacga tgaacttgcc taacagatca cgtgcgacct gcagagtggg
     1861 tcggttgaag gcggcgcggt cgaggcggcg tggctcctgt cgtttcaccc gctctttggg
     1921 ggcgaccgca tcaccagcgg atttcgtctt tgccgtcatt tcacacaggt gaaatcaaga
     1981 acacgtgcat tgctttgttc tctctatcag ggcgctcccg catgatagaa cacggatgaa
     2041 ccacggagga cacggaggac acggaggaag gacaagagca acaaattatt tgagtaggaa
     2101 cgcttccatg gctgatgatc cgtccccgca aaacaggcca gcacgggatt gcgaacaaca
     2161 acattgctct taaccctagt tttctctttc ttctttacct ccgtgtcctc cgtgtcctcc
     2221 gtggttcatt ggtatttcag gttttgctct tccgaccggt gcgcgaggcg agcacgcgat
     2281 cgatgaattg ccccgccacg ccgagatagt tggcggggtc cagcaagcga tcgaggtcgg
     2341 acggggaaag cagcttgctc acctttgtgt ctttcgcgag tacgtcgcgc aaaggcttct
     2401 gttgctcgat cgcgcggcgg caggccgact cgatcaattg gtgcgaggta tcgcggccga
     2461 tctttggagc gagtgcggcc gacaccgctt ccgcaagaat taatccgtgt gtggcgtcga
     2521 ggttatggcg catacgcgcc gtgtcaatct ccatgccttc gatcatctga acggtctgag
     2581 ccagcgctcc agcacagagc atgcagatct cgggcagcgt ctcccactcc gcatgccagt
     2641 tgccgagccc gcgctcgtgc tcctgcacca tcgccgcaag catcaccgac accagcgccg
     2701 gcaccttggt tgccgcggca aggaccacgg cagcacccac cggattgcgc ttgtgcggca
     2761 tggtcgacga tccgcccttg cctggcgcgg cgggttcgaa cgcctcggcc atctcggtct
     2821 gcatcagcag cgacatgtct tttgcgatct tccccagcgt ccctgtgagc aggccgatag
     2881 tcgtggcgac ttccgccatg cgatcccggt gcgcgtgcca gggcaggttc ggttcgccga
     2941 gctgcagttc cgtggccagt gccttggcca cgtcgaggcc gcgcccgccg agcgacgcga
     3001 gcgttccggc cgcaccgccg aactggatga ccagcacgcg tgcgcgcaat tcgcgcagcc
     3061 gcgcgcggtg cctttcgact gcgctgagcg cgcccgcagc cttcaaaccg aatgtgaccg
     3121 gcaccgcctg ttgcagccag gtgcgtcccg ccatcggcgt gcgtttgtgt tttatcgcga
     3181 ggcgcgccag tgcgcccgca agttgcgcga gatcggcgtc gaagaggtcg aacgcagcgc
     3241 gcaactgcag gaccagaccg gtgtccatcg catcctggct ggtcgccccc cagtgaacat
     3301 agcccgccac ttgcgcgtcc tttttcgcga ccagggcagt cagcgccttc actgtcggaa
     3361 tcgcgggatt gccggcaagc accgtcgcgc gcgcgagcgc ttcgacatcg aacagttccg
     3421 cccggcactg cgcttcgatc accgggacgg cgttcaccgg aatgacgccg acgcgggctt
     3481 cggcccgtgc cagcgctgcc tcgaagtcca gcataccctg caggcgcttc cgatcggaga
     3541 agatctcgcc gacggcatcg gcgcgaaaca gcgcgtcgag caatccgctc atgtcagtag
     3601 ctgaagaaca cggtttcgtg gtcgccttgc aggatcacgt cccattccag gccgtcggcc
     3661 gtcttcctgg ccaccagcgt ctggcggcga tcggcgtgaa cgagcgccag gatcgggtcg
     3721 tcggcattcg ccgtctcgcc gggaaagtag atgcgcgtga gcaagtggat cagcaatccg
     3781 cgcatgaaca cggcgaccag aagatggggc gcctgcggca tgccgtgggg gccggccacg
     3841 ctgcccggct tgatggtgat gaaccggaac acgccgttat cgtcggtggc tacacggccg
     3901 aatcccctga aatccggcgt caccggttta tcctgcgcgt cgtcgggatg ggcgtatttc
     3961 ccgtgcgcgt tggcctgcca gatttccagg atggcatcgt tgaccggctt gccatcgccg
     4021 tcgaggacgc gcccctggaa cgcaatcttg tcggcagcgg gcgcagcggg cgccagatcg
     4081 ctgacgtaga gcgcctccag gccgatgcga aaaaacggcc cgacggtctg ggatgcggtg
     4141 gccggcaggt tcatcgggtc ttttccatcg gtgtcgcgtc ccggcctcgc agcacgatat
     4201 cgaatcggta gcccagcgcg tgttcgggta cagtgatgtc ccaatcgaaa tcggccacca
     4261 gccggtggcg cgcgccttca tttgcggtgc cgttgaaaat cggatcgtgc ggcaacaacg
     4321 ggtccccggg aaaatacatc tgcgtgacca gccgcgtcac gaaagcgcgg ccgaacagcg
     4381 agagatggat atgggccgga cgccaggcgt tgtgatggtt gcgccacgga taggcgcccg
     4441 gcttgatggt gacgaagcga tagcggccgt cctcgtcggt cagcacgcgg ccggcaccgg
     4501 agaaattcgg atccagcggc gcatcatgat tatcgttctg gtgccggtag cggcctgcgg
     4561 cgttgcactg ccagatctcg accagcgcgt tcggcacggc gcgcttgttt tcgtcgagga
     4621 cccggccgcc gacgatga
//
LOCUS       DATGEM010000633        13189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_342432, whole genome shotgun sequence.
ACCESSION   DATGEM010000633 DATGEM010000000
VERSION     DATGEM010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13189
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342432"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VKQ27_19460"
     CDS             complement(<1..349)
                     /locus_tag="VKQ27_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLY91171.1"
                     /translation="MKKPNGEPPLLDAIDRRILAELQADGRMTNVELARRAGISAPPC
                     LRRVRRLEEAGIIRGYHADPDPQKLGWEITVFAIVGLDSQKEAVLSAFEQLVAGWPEV
                     RECHMIRGGGDFLL"
     gene            547..1515
                     /gene="trxB"
                     /locus_tag="VKQ27_19465"
     CDS             547..1515
                     /gene="trxB"
                     /locus_tag="VKQ27_19465"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLY91172.1"
                     /translation="MPQTHRTRVLIIGSGPAGYTAAIYAARASMSPILVAGLQAGGQL
                     TITTDVENYPGFADVIQGPWLMEQMAAQAKHVGTQIVEDLITRVDFSTWPFSCVSDSG
                     DTFLADSVVIATGAQARWLDIDSEERLQGRGVSACATCDGFFYRGKRVAVIGGGNTAV
                     EEALYLTHHADSVTLIHRRDSLRAEKILQQRLFANPKIGVIWDSVVEEIEGGGTPEVV
                     TGVRLLNKKTGAMSSHPFDGVFIAIGHTPNTAIFAGQVTMDAEGYIQTQPGSTRTSVE
                     GVFAAGDVQDKIFRQAVTAAGSGCMAALEAEKWLATRTVTPALAAD"
     gene            1678..2562
                     /locus_tag="VKQ27_19470"
     CDS             1678..2562
                     /locus_tag="VKQ27_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLY91173.1"
                     /translation="MDWDKLRVFHAVAEAGSFTHAGDTLNLSQSAVSRQISALEEALQ
                     VPLFHRHARGLILTEQGEALNRTVREVFAKLAITEALLTESKEKPAGRLKVTTTHAFG
                     SIWLAPRLHEFLAMYPDVSVSLLLDDAELDLAMREADVAIRMHPPKQPDLVQRHLLTL
                     QWHVCASTDYLKQQGVPQRPEDLDAHKLILFGSHHPPVLEVNWLAEVGRRPGNPRRAL
                     LEVNSTQAVLLAIRSGLGIGALPDYVVNENPDLVRILPELKGPRVDVYFVYPEELRNS
                     KRVAVFRDFLLARLAQIH"
     gene            complement(2890..3954)
                     /locus_tag="VKQ27_19475"
     CDS             complement(2890..3954)
                     /locus_tag="VKQ27_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HLY91174.1"
                     /translation="MRRIGYCVLFVLMLFAWHANADPPAALRRGVNITNWFRFPPNRD
                     PAVLRAYLDDAAISRIRQAGFTFVRLAVQSDLLSETEPLRDAVGRLERQGLAVVVALF
                     PHGWQPETAPERLLAAWRSLAPLLRSFDPARTFPETLNEPVFADAPEDWPRLQREVLA
                     AIRSALPLNTVILTGADWGSVRGLLALSPDPDPNVVYSFHYYEPAELTALGAYRLGLD
                     RTAMARLPFPAADPATCEKAAAVTEDTMTIGLIRFYCALHWDANKIAARIAQVGTWAA
                     RNHVAVIAGEFGASRELNRESRAAWLAAVRDACEQHGLGWALWGYDDSMGFAAKPAAA
                     SGLDPVVLRALGLSESQPAK"
     gene            3999..5447
                     /locus_tag="VKQ27_19480"
     CDS             3999..5447
                     /locus_tag="VKQ27_19480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY91175.1"
                     /translation="MRAGKIFAVMLLAAAVLTAAGWLRGAEYDEQYTLFLTGGTVRPA
                     WPSDAISAADVLASQDGRTSLAAIARGLRASDVHPPLYFWLIALWRRLVGGGLFAARM
                     FSVVCGVAALGLVGAIARQAAVPTTAAMLLTLGCYGFAFSDAVARGFALAQMLNLAGV
                     ALLLFAPRRRRCAALCSGLLLGAAGFTNYLAAFVGCAGLLWTVLAFRRSWIWTALGFA
                     IWLPADLWFFLAQRNSRIGQFEPFSLASGLVRLIRYGTAAVFGGLPLYVSGLASQAVG
                     MLLAALMLALLAVAIIRWRRVHSLFAMAAVATPLGLLLLGLAFDTTPIELRYLAFATP
                     FIGLVLASVLPRAAVCVVLAIQAISLAGLMTRAETMQPARMAARDATRLAGDGVVLVP
                     HGNDGVGIVGAFAREAPATLRLLVVRRDDSPAQILTRTGNASHVVLALLGQDADSRAA
                     LPHMREAFASPCWRPAGQGFNVLAFERICGGE"
     gene            5450..6328
                     /locus_tag="VKQ27_19485"
     CDS             5450..6328
                     /locus_tag="VKQ27_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLY91176.1"
                     /translation="MFFEGFALAEVEVPGGSIRLRHGGSGPPLLLLHGNPQTHAMWHL
                     IAPTLARRFTVICPDLRGYGFSHKPPATSDHMPYSKREMAKDMIAVMDHLGHQRFLLA
                     GHDRGGRLSHRIALDYPDRVEKLATLDIVPTIEAFERTDMTYAMNHYHWFWLAQPHPF
                     PEWLINKAPEEWWRQHTNRNPDRADLFQPEALADYLAAVRIPETVRGICEDYRAAISI
                     DLVHDRASREAGEKILCPLLAVWGKRGKIEKSFDALAIWRQYCANDVIGGAIESGHYI
                     AEENPAALLAWFDRFF"
     gene            complement(6560..7738)
                     /locus_tag="VKQ27_19490"
     CDS             complement(6560..7738)
                     /locus_tag="VKQ27_19490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY91177.1"
                     /translation="MKTQRLGSCLIAAVAAATLTTTPMAQTVQGVTDTEIVIGTATDL
                     SGVTAVQGVNNSNSVRLAFDEANAAGGVNGRKIKYIVEDSQYQVPRAVQAMNKLLNED
                     HIFFATVNGGTPMNDAMMPMMFAKGVPNLLPLTAARSMYEPYNKLKWSQFASYYDQMR
                     AGVKYFVEQHGKKMVCAMYQDTDFGKDVLAGVVAQLDAEHLKLIASTGHKPTDTDFTA
                     NLSKLRDAGCDLITMGTIVRDSTLIVSTTKKMGWNVDLLGQVASYDTAVATAPGGVGE
                     GFYSMTPALYAYPDDPRPAVADFAKRYKAKFGIDPNFLGETGYTTAQIVLEALKRAGR
                     DLNVDTLTKAMESIHDFHDIFGNSFSFGPDQHHGATSAYLAVVKNGRWVPVVDHALAY
                     "
     gene            complement(7813..8331)
                     /locus_tag="VKQ27_19495"
     CDS             complement(7813..8331)
                     /locus_tag="VKQ27_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HLY91178.1"
                     /translation="MISPAYVRLMAEYNAEMNRRLYAAAGRLTDAERKADRGAFWGSI
                     HGTLNHLLWGDTQWMSRFDNWEKPPVSIKQSAGMIHDFATLQARREKADAAIIDWASR
                     ADDAWLTEDLVWFSGAAQREMRRKKGPLVVHFFNHQTHHRGQVHALLTACGQDTGDTD
                     LFLVVPEALAAT"
     gene            complement(8427..9461)
                     /locus_tag="VKQ27_19500"
     CDS             complement(8427..9461)
                     /locus_tag="VKQ27_19500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLY91179.1"
                     /translation="MKVVLLGTEFAMNGAAVLLYRWAMHLVQRGHTVSAVPASQKATA
                     VGPLRDAYVAAGVTIEEKLYVDPQTLVICNTIAAAPYVIQTAPSARTIWWLHEGEVGL
                     ELLFANPGAARAFHAAHAVIFPSAVIRDQVYASYLLGVPKQRLHVVPPGLEPLEQQAT
                     ETEPQGEKRPLRVVCIGSIYPRKRQADLIKAIALLRNVPIECQLVGQMVSLEEEATEI
                     ARKSPKRFSFVGQLPHVETMRLLDRADVLALPSSSECLPIAPLEAGQRGKAILLSDLP
                     AHEGIWRHGINCLMHPVGDVTLMSHSLRILASDRQLRERLGAQARRVAAPYRNDILLA
                     RLSLVIASLT"
     gene            complement(9468..10670)
                     /locus_tag="VKQ27_19505"
     CDS             complement(9468..10670)
                     /locus_tag="VKQ27_19505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLY91180.1"
                     /translation="MPGFANRLSRVQVAASVQMTIRARELRAQGVDIIQLTIGEPNFA
                     SPTHAIEAAHEAALKGETKYPPQEGIAPLKQAIQRKFKRDSGLDFALDEICVTNGGKQ
                     AIFNALGATLNQGDEAVIPVPSWSAYALMTKLFDGEPVLVTCPQNNGFKPRAEDIDAA
                     ITPRTKWLILNNPNNPTGACCSAQELRAIADVMLKHPHVWIMSDDMYEHLVFDGFRHE
                     TIAHIAPELRDRTVTITGASKAYAMTGWRIGFAGGPKDLIKAMVNMQGQATAGVSTVG
                     QAAAVAALNGPQDGVQEQVIAYARRRDMTVEMLNTCKGINCHKPEGAFYVFPNVAGCI
                     GKTTAGGRRLETDRDVCMALLEEAHVATVHGAAYGMSPYLRVSTATDDDSLAEGCRRI
                     KAFCEALH"
     gene            complement(10670..11872)
                     /locus_tag="VKQ27_19510"
     CDS             complement(10670..11872)
                     /locus_tag="VKQ27_19510"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLY91181.1"
                     /translation="MQLTAERLDRIQPSLTIAIATKARHMIAEGKNVVSLSQGEPDFD
                     TPRNVKDAAIRAIEANETHYTDVAGTPELRKAVAAKFKRDSGIDYKPEEIIISTGGKQ
                     VIFNAMLATLNKDDEVIIPSPCWVSYPDIVSLADGKPVLVPCEQNHGFKLRAEALEQA
                     ITPRTKWFMLNNPCNPTGAAYSAEELRPICDVLLRHPDVWIFTDDIYEKLAYDGFKPA
                     TIVEVEPRLKDRTVTMNGCSKAYAMTGWRIGFAGAPVSLIKAMDKLQSQSTSNTNSIA
                     QAAAVEALTGPQESIEAMRKIFEERRDLVVDMLNKCPGLYCHKPEGAFYVYPSIHGCI
                     GKTTRGGKKIADDESFVLAMLEETGVAVVHGSAFCYPGHFRLAYANATEELQDACTRI
                     RQFCEGLH"
     gene            12042..12500
                     /locus_tag="VKQ27_19515"
     CDS             12042..12500
                     /locus_tag="VKQ27_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY91182.1"
                     /translation="MSIVPIRGLFEAHLTVSDLDRSIAFYRDVLGLPLAHRIPARNVA
                     FFWVPTSDKAMLGLWSIGTSPLRLRLHIAFDVALPQVFESVAALRRAGITPRSGDGAA
                     IEEPIVLAWMPAASVYFDDPDGHSLEFICMLDEPPRPELGQVSWSEWRRG"
     gene            12497..>13189
                     /locus_tag="VKQ27_19520"
     CDS             12497..>13189
                     /locus_tag="VKQ27_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLY91183.1"
                     /translation="MSAEIRVERREGWRKLVFNRPDKLNAVNEAMLHALIAALNEAEA
                     EGDCRAVLLTGEGRGFCTGQELGPAVMPGPHGAPDLGAVAELHHALVRRLRAVPMPVV
                     CAVNGIAAGAGASYALACDIVLAARSARFIQAFVKIGLVPDSGASFFLPRLVGEARAR
                     ALAMLGDAIDAERAEAWGMIWKVVDDAALQTEAEALTANLARQPTASLAAMKRMFAAA
                     TANSLEAQLDLER"
BASE COUNT         2121 a         4154 c         4428 g         2486 t
ORIGIN      
        1 tcagcaggaa atcgcctccg ccacggatca tgtggcactc acgcacctcc ggccagcccg
       61 cgacgagctg ctcgaaggcg gacagcacgg cttccttctg gctgtccagt ccgacaatgg
      121 cgaacacggt gatctcccaa ccgagcttct gcgggtcggg gtctgcatga taaccgcgga
      181 tgatgccagc ctcctccagc cggcggacgc ggcggaggca gggcggggcg gaaatgcccg
      241 cgcgccgggc cagctcgaca ttggtcatcc ggccgtcggc ctgcaactcc gcgaggatgc
      301 ggcggtcgat ggcatcaagt agcggcggtt cgccgttggg ctttttcata cgctgggttg
      361 gaccgggcgc tgctggcgct gttctgagtg tgacatttcc ggggacggcg tgacgggcca
      421 atgatatggc aggggacgat ggccgcaagt gggcaccgaa ggggtgtgtc catggtgcgc
      481 ggcacgcccc gtgaactccg tgtcgtcaca ccctataatt gctgcgtccc ctcgactgga
      541 atcgccatgc cccaaaccca tcgcacgcgc gtcctgatca tcggctccgg cccggccggc
      601 tacactgctg cgatctatgc cgcgcgagcc agcatgtcgc cgatcctggt ggccggcctg
      661 caggcgggcg gccagctcac gatcaccacc gacgtggaga actatccagg cttcgccgat
      721 gtgatccagg gcccctggct catggagcag atggcagcac aggccaagca tgtcggcacg
      781 cagatcgtcg aagaccttat tacgcgggtc gatttctcga cctggccatt ctcctgcgtg
      841 tctgactcag gtgacacgtt ccttgccgac tccgtggtga ttgccaccgg cgcccaggcc
      901 cgctggctcg acatcgactc ggaggagcgg ttgcagggcc gcggcgtctc tgcctgcgcc
      961 acctgcgacg gcttcttcta tcgtggcaag cgtgtcgcgg tgatcggcgg cggcaacacg
     1021 gcggttgagg aagcgcttta cctcacccac cacgccgaca gcgttacgct gatccaccgc
     1081 cgcgattcgc tgcgcgcgga aaagatcctg caacagcggc tgttcgccaa tccgaagatc
     1141 ggggtgatct gggacagcgt ggtggaagag atcgagggcg gcggcactcc cgaagtcgtt
     1201 accggcgtcc ggctgctgaa caagaagacg ggcgcgatga gcagccatcc gttcgatggt
     1261 gtgttcatcg ccatcggcca taccccgaac acggcgatct tcgccggcca ggtcacgatg
     1321 gatgccgagg gttacatcca gacccagccc ggcagtaccc gcacgtcggt cgagggcgtg
     1381 ttcgccgccg gggatgtgca ggacaaaatt ttccgccagg cggtcaccgc ggcgggctcc
     1441 ggctgcatgg cggcgctcga ggcggagaaa tggcttgcaa ctcggacggt gacaccggcg
     1501 ctcgccgcgg actagtctgg attccgccaa taggtccaat cgcgaccagc cgaatcctgg
     1561 aagatcgact aacgtcccag ggtccgaccc agtaacgaga cggcaacgac aggcaccagg
     1621 gtaggctttg cggaacacca ccgatgatca ggagcggtgg gcgggagagc gcgagtgatc
     1681 gactgggaca agttgcgggt gttccacgct gtggctgagg ccggcagctt cacccatgcc
     1741 ggtgacacgc tgaatctcag ccagtccgcc gtgtcgcggc agatttcggc gctcgaggaa
     1801 gccttgcagg tgccgctgtt ccaccgtcac gcccgcggcc tgatcctgac cgagcagggc
     1861 gaggcgctga accgcaccgt gcgcgaggtg ttcgccaagc tggcgatcac cgaggcgctg
     1921 ctgaccgaga gcaaggaaaa accggccggc cgcctgaagg tgaccacgac ccacgccttc
     1981 ggctcgatct ggctggcgcc gcggctgcac gaattcctgg cgatgtatcc ggacgtctcg
     2041 gtctctctgt tgctcgatga cgccgagctc gaccttgcga tgcgcgaggc ggacgtggcg
     2101 atccgcatgc acccgccgaa gcagcccgac ctggtgcagc gacacttgct gacgctgcaa
     2161 tggcacgtct gcgcctcgac cgactacctg aagcagcagg gggtgccgca acggcccgag
     2221 gacctggacg cccacaagct gattctgttc ggcagccacc atccgcccgt tctggaggtg
     2281 aactggctgg ccgaggtcgg gcgacgcccc ggcaatccgc ggcgggcgtt gctggaagtg
     2341 aacagcaccc aggcggtctt gctggcgatc cgctccggcc tcgggatcgg cgccctgccg
     2401 gactatgtag tgaacgagaa tcccgacctc gtccgcatct tgccggagct gaaggggccg
     2461 agagtggacg tctactttgt ctacccggag gagctgcgga attcgaagcg agtggccgta
     2521 ttccgcgact ttctgctggc gagactcgcg cagattcatt gatctgccgc ctcggccgcg
     2581 agcaccaatg cgttaacagt tggtgacagg ctgggccatg cgcagcaagc atggcggcat
     2641 ccggcttttg gcgttggcca ggctcgcggc agcctcttaa agttaatgca tcgaatacgg
     2701 cggcgttgcc gccagtgttc cggcctctcg gggtcggaag ggggtctatg acccctgcgt
     2761 ctcccagacg tgaagcctcc ctgtttactt acccccggcc ttgagccggg ggtttttctt
     2821 tttctcgatt gattgacgct cgcctcacgc gctggatcga ctgtcagtgg ccaggggctg
     2881 tgcttggtac tatttggcag gctgcgactc ggacaggccc aatgcgcgca agacgaccgg
     2941 gtcaagcccg cttgccgccg caggctttgc ggcgaacccc atcgaatcgt catagcccca
     3001 cagcgcccat ccgagtccgt gctgttcgca ggcgtcgcgc acagcggcga gccaggctgc
     3061 ccttgattcg cggttcaact cccgggatgc gccgaattcg ccagcgatca cggccacgtg
     3121 gttgcgcgca gcccaggtgc ccacctgggc gatccgggcc gcaatcttgt tcgcatccca
     3181 gtggagcgcg cagtagaagc ggatcaggcc gatggtcatc gtgtcctcgg tgaccgccgc
     3241 ggccttctcg caggtagcgg gatcggcggc gggaaacggc aggcgcgcca tggccgtccg
     3301 atccagtccg agccggtagg cgcccaatgc agtcagttca gctggttcgt agtagtggaa
     3361 gctgtagacg acgttcggat cgggatccgg cgacagcgcg agcaggccgc gcacgctgcc
     3421 ccagtctgca ccggtgagga taaccgtgtt cagaggcagg gccgatcgga tggctgccag
     3481 cacttcacgc tgcaaccttg gccagtcctc gggggcatcg gcaaatacgg gctcattcag
     3541 ggtctccggg aacgtccgtg ccggatcgaa actgcgcagc agtggcgcga gcgagcgcca
     3601 tgcggccagc agccgctccg gcgcggtttc gggctgccac ccatgcggga acagcgcaac
     3661 gaccacggcg aggccctggc gctccagccg gcccacggca tccctgagcg gttcggtttc
     3721 tgacagcagg tcggactgga ccgccaggcg gacaaaggtg aagccagcct gtctgatgcg
     3781 gctgatcgcc gcgtcatcaa gataggcgcg caggaccgcc ggatcccggt tgggcggaaa
     3841 gcggaaccag ttggtgatgt tcacgccccg gcgcagtgcc gcaggcgggt ccgcgttggc
     3901 gtgccaggca aatagcatca gcacgaacag gacgcagtaa ccaatccttc gcatcgtgct
     3961 gccatacacc gccctgcttt aacgcccggt gaaggcgcat gcgtgcgggg aagatattcg
     4021 cggtgatgct gttggcggcc gcggtactga ctgccgccgg atggcttcgc ggcgccgaat
     4081 acgatgagca atacacgctg ttccttaccg gcgggaccgt gcggccggcc tggccgagcg
     4141 atgctatttc cgccgcggac gtgctggcgt cgcaggatgg ccgaaccagt cttgcggcaa
     4201 tcgcacgggg cctgcgggcg agcgacgtac acccgccact gtatttctgg ctgatcgcgc
     4261 tatggcgccg gctcgtgggc ggcgggctgt tcgccgctcg catgttttcc gtcgtgtgcg
     4321 gtgtggccgc gcttggattg gtcggcgcga ttgcccggca agcggccgtg ccaacgactg
     4381 ctgcgatgct gctgacactg ggctgttatg gctttgcctt cagcgatgcg gtggcgcgcg
     4441 gctttgcact ggcgcaaatg ctgaacctgg ccggcgtcgc gctcctgttg tttgcgccac
     4501 ggcgacgtcg ctgtgcggcg ctgtgcagcg gcctgttgtt gggagcggcg ggcttcacga
     4561 actatctggc ggcgttcgtt ggctgtgccg gcttgctatg gacggtcctc gcattccggc
     4621 gttcgtggat ctggacggcg ctcggcttcg ccatctggct gccggccgat ctctggttct
     4681 tcctggcgca gcgcaacagc cggatcgggc agtttgaacc gttcagcctc gcatcgggtc
     4741 tggtccggct tatccggtat ggcacggcgg cggtgtttgg tgggctgccg ctctacgtct
     4801 ccgggctggc cagccaggct gtcgggatgc tgctcgccgc actgatgctg gcgctgctgg
     4861 ccgtggcaat catccgctgg cgccgtgtcc attcgctgtt cgcgatggcg gcggttgcga
     4921 caccgctcgg cctgttgctg ttgggcctcg cgttcgacac cacacccata gagttgcgct
     4981 acctggcatt tgcgacgccc ttcatcggcc tggtgcttgc gtcagtgctg ccacgcgccg
     5041 cggtctgcgt cgtattggcc atccaggcga tatctttggc cggactgatg acgcgcgcgg
     5101 agaccatgca gccagcccgc atggccgcgc gcgacgcgac caggctggcg ggcgatggcg
     5161 tcgtcctggt gccgcacggc aatgatggcg tcggcatcgt cggcgccttt gcgcgcgaag
     5221 cgcccgcgac gctgcgcctt ctggtggttc gccgtgatga ctcgccggcg cagatcctga
     5281 cgcgcacggg gaatgcatcg catgtcgtgt tggcactcct tggccaggat gccgacagcc
     5341 gcgccgcact gccacacatg cgcgaggcct tcgccagccc gtgctggcgt ccggccgggc
     5401 aggggttcaa cgtgctcgcc ttcgagcgga tctgtggagg ggaatgagca tgttcttcga
     5461 aggcttcgcc ttggccgagg tcgaggtgcc cggtggcagt atccgcctcc gccacggcgg
     5521 ttccggtccg cccttgctgc tgctgcacgg caatcctcag acgcacgcga tgtggcatct
     5581 gatcgcgcca acgctggcgc ggcgcttcac cgtgatctgc ccggatctgc gcggctatgg
     5641 gttctcgcac aagccgccgg cgacgagtga ccacatgccg tattcgaagc gcgagatggc
     5701 gaaggacatg atcgccgtca tggaccatct gggtcatcag cgcttcctgc tcgccggaca
     5761 tgatcgcggt ggccggctat cgcacaggat cgcgctcgac taccccgacc gcgtggagaa
     5821 gctggcgacg ctcgacatcg tgccgacgat cgaggcgttc gagcgtaccg acatgaccta
     5881 cgcgatgaac cactaccact ggttctggct ggcgcaaccg catccgttcc cggaatggct
     5941 gatcaacaag gcgcccgagg aatggtggcg ccagcacacc aaccgcaacc cggatcgcgc
     6001 cgacctgttc cagcccgagg cactggcgga ctacctggct gcggtgcgca ttccggagac
     6061 cgtgcgtggc atctgcgagg attatcgcgc ggctatcagc atcgatctgg tgcacgatcg
     6121 tgccagccgc gaggcgggtg agaagatcct ctgcccgctg ctcgcggtat ggggcaagcg
     6181 cggcaagatc gagaagagct tcgacgcgct ggcgatctgg cggcagtatt gcgccaatga
     6241 cgtgatcggc ggcgcaatcg agtcaggcca ttatatcgcg gaggaaaatc ccgccgcgct
     6301 gctggcctgg ttcgaccggt tcttctgagg gagaggaagg ccaaggggac tttgccccct
     6361 tggatccccc actaagggcg gagcccttag aaccccggtt ttgtccgtcg ttggaagggg
     6421 ccatcagggg atgaacatat cttgcgatgg ccccctcccg acgacagaca ggacgggttc
     6481 caaaggcgcc gcctttggcg aggattgggc cgcgaagcgg accaatgggg aacagagctc
     6541 ccctggcctt tcctgctgct cagtaggcca gggcgtgatc aaccaccggc acccagcgtc
     6601 cgttcttcac caccgccaga tacgcgctgg ttgcgccatg gtgctggtcg ggaccgaagc
     6661 tgaagctgtt gccgaatatg tcgtggaagt cgtggatgct ctccattgcc ttggtgagcg
     6721 tatccacatt caggtcgcgg ccggcacgct tcagcgcttc cagcacgatc tgcgccgtgg
     6781 tgtagccggt ctcgccgagg aagttgggat cgatgccgaa cttcgccttg tagcgcttgg
     6841 cgaaatccgc cactgccggt cgcggatcat cgggatacgc atacaacgcg ggcgtcattg
     6901 aatagaaccc ttcgcctacg ccgcccggtg ctgtcgccac cgcggtatca tacgacgcga
     6961 cctgaccaag cagatcgacg ttccagccca tctttttcgt cgtcgagacg ataagcgtgc
     7021 tgtcgcgcac gatggtgccc atggtgataa ggtcgcagcc cgcgtcgcgc agcttgctca
     7081 gattcgcggt aaagtcggtg tcggtcggct tgtgcccggt ggaggcgatc agtttcaggt
     7141 gctcggcgtc gagctgcgcc accaccccgg ccaaaacatc cttgccgaag tcagtgtcct
     7201 gatacatcgc gcagaccatc ttcttgccgt gctgctcgac gaagtatttc acgccggcgc
     7261 gcatctggtc gtagtacgac gcaaactggc tccacttcag tttattgtac ggctcataca
     7321 tcgagcgcgc ggcggtcagt ggcagcaggt tcggcacgcc cttggcgaac atcatcggca
     7381 tcatcgcgtc gttcatcggc gtgccaccat tgaccgtggc gaaaaagatg tggtcctcgt
     7441 tcagcagctt gttcatagcc tgcacggcac gcggcacctg gtactggctg tcctcaacga
     7501 tgtatttgat cttgcggcca ttgacgccgc cggccgcatt ggcctcatcg aacgccaggc
     7561 gcaccgaatt cgagttgttc acgccctgca ccgcggttac gccggagagg tcggtggccg
     7621 tgccgatgac aatctcggta tcggtgacgc cctggacggt ctgcgccatc ggggtcgttg
     7681 tgagcgtcgc cgccgcgacg gcggcgataa ggcacgagcc aagtcgttgg gtcttcactt
     7741 cgatgcatcc tcctgtttcg cggcggccat tcgtggcggg ctgcctgcat gggaggaagc
     7801 ctattacacc attcaggtgg cggccagcgc ctccggaacg accaggaaca gatcggtatc
     7861 gccggtgtcc tggccgcacg cggtcagcag ggcatgcacc tggccacgat ggtgcgtctg
     7921 gtgattgaag aagtgcacga ccagcggccc cttcttccgc cgcatctcac gttgcgccgc
     7981 gccgctgaac cagaccaggt cctcggtaag ccacgcatca tcggctcggc ttgcccaatc
     8041 gatgatggcg gcatcggctt tctcgcgccg tgcctggagc gtggcgaagt cgtggatcat
     8101 gccggcactt tgcttgatgc tgaccggcgg cttttcccag ttgtcgaagc gcgacatcca
     8161 ctgcgtgtca ccccacagca ggtggttcag ggtgccatgg atcgaacccc agaacgcgcc
     8221 gcgatcggcc ttgcgttctg cgtcggtgag acgcccggcg gccgcgtaga gacggcggtt
     8281 catctcagcg ttgtactcgg ccatcagacg gacgtaagct ggtgagatca tgacgggatt
     8341 tctgcctagt ggcggcgccg cgtcaatctt gatcgagtga atcaactcca cctgccattg
     8401 catcgctccg ctcgcagtga cagccgctag gtcaggctgg caatcaccag gctgagccgc
     8461 gcgagaagta tgtcgttgcg atacggtgcc gcgacgcgac gtgcctgtgc gccaagccgc
     8521 tcgcggagtt gccggtcgct tgcaaggatg cgcagtgagt gcgacatcag ggtcacgtcg
     8581 cccaccggat gcatcaggca gttgatgcca tggcgccaga tgccttcgtg tgccggcaga
     8641 tcggacagca ggatcgcctt gcctcgctgg ccagcctcga gtggcgcgat cggtaggcat
     8701 tcgctgcttg atggcagcgc aaggacgtcg gcgcgatcga gcagccgcat cgtctcgaca
     8761 tgtggcagct ggcccacgaa gctgaagcgc ttcggcgact tgcgggcgat ctcggtggct
     8821 tcttcctcga ggctcaccat ctggccgacc aactggcatt cgatcggcac attgcgcagc
     8881 agcgcgatgg ccttgatcag gtcggcctgg cgtttgcgcg ggtagatcga accgatgcag
     8941 acaacccgga gcggccgctt ttctccttgc ggttctgtct ctgtggcttg ttgttccagc
     9001 ggctcgagac ctggcggtac gacgtgcagg cgctgcttcg gcacacccag cagataggac
     9061 gcatatacct ggtcgcggat caccgccgat gggaagatca ccgcgtgcgc cgcgtggaac
     9121 gcacgcgccg cgccaggatt ggcaaacaac aactcgaggc cgacctcgcc ctcgtgcagc
     9181 caccagatcg tgcgtgcgct tggcgcggtc tggatgacat aaggtgccgc tgcgatcgtg
     9241 ttgcagatca ccagcgtctg tggatcaacg tagagctttt cctctatcgt cacgcccgcc
     9301 gcgacatagg catcgcgcaa cggtcccacc gcggtggcct tctgcgatgc cggcacggca
     9361 ctgaccgtat ggccgcgctg caccagatgc atcgcccagc ggtacaacaa tactgccgca
     9421 ccgttcatgg caaactcggt gccgagcagc acgaccttca tgggaggtca atgcagcgct
     9481 tcgcagaacg ccttgatgcg gcggcagcct tccgccagtg aatcatcatc cgtggcagtg
     9541 ctgacgcgca ggtatgggct catgccatag gccgcgccat ggaccgtggc gacatgcgct
     9601 tcttccagca aggccatgca gacgtcgcga tcggtctcca gtctgcgccc gcccgccgtg
     9661 gtcttgccga tgcagccggc gacgttcggg aacacgtaga acgcgccttc aggcttgtgg
     9721 cagttgatgc ccttgcaggt attcagcatc tccaccgtca tgtcgcggcg gcgcgcatag
     9781 gcgattacct gctcctgcac gccgtcctgc ggcccgttca gcgcggcgac cgcggcggcc
     9841 tggccgacgg tagatacgcc cgccgtcgcc tggccctgca tgttgaccat cgccttgatc
     9901 aggtccttcg ggccgccggc gaagccgatg cgccagccgg tcatcgcata cgccttcgag
     9961 gcgccggtga tcgtcaccgt gcgatcccgc agctcgggtg cgatatgtgc gatcgtctcg
    10021 tgcctgaagc cgtcgaacac gaggtgttca tacatgtcgt ccgacatgat ccagacatgc
    10081 ggatgcttca gcatcacgtc ggcgatcgcg cgcagctcct gcgccgagca gcaggcaccg
    10141 gtggggttgt tcgggttgtt caggatcagc cacttggtcc tgggcgtgat cgccgcatcg
    10201 atgtcctcgg cgcggggctt gaagccgttg ttctgcgggc acgtcacgag caccggctcg
    10261 ccatcgaaca gcttcgtcat cagtgcatag gcgctccagg acggaaccgg gataaccgcc
    10321 tcatcgccct gattcagcgt ggcccccaga gcgttgaaga tcgcctgctt gccgccgttg
    10381 gtcacgcaga tctcgtccag cgcgaaatcc agcccgctat cgcgcttgaa cttgcgctgg
    10441 atcgcctgct tcagcggcgc gatgccttcc tgcggcggat acttggtctc gcccttcagc
    10501 gccgcctcat gcgccgcctc gatcgcatgc gtcggggagg cgaagttggg ctcgccaatc
    10561 gtcagctgga tgatgtcaac accctgcgcc cgcagctcgc gcgcgcgtat ggtcatttgt
    10621 actgacgcgg caacctgcac ccggctgagc cggttggcga atccgggcat cagtggaggc
    10681 cttcgcagaa ttgccggatg cgcgtgcagg cgtcctgcag ctcttcggtc gcgttggcgt
    10741 aggccaggcg gaagtgcccc gggtagcaga acgcggatcc gtgcacgaca gcgacgccgg
    10801 tctcctcaag catcgccagc acaaagcttt cgtcgtcggc gatcttcttg ccgccgcgcg
    10861 tcgtcttgcc gatacagcca tggatcgagg gatatacgta gaacgcgccc tccggcttgt
    10921 ggcagtagag gccagggcac ttgttcagca tgtccaccac gaggtcgcgc cgctcctcga
    10981 agatcttgcg cattgcctcg atcgattcct gcgggccggt cagggcttcc actgccgccg
    11041 cctgagcgat cgagttggtg ttggaggtgg actggctttg cagcttgtcc atcgccttga
    11101 tcagcgacac gggcgcgccg gcgaaaccga tacgccagcc ggtcattgca taggccttgg
    11161 agcagccgtt catcgtgacg gtgcggtcct tcagccgcgg ctccacctcg acgatcgtcg
    11221 ctggcttgaa gccgtcatag gcaagttttt cgtagatgtc gtcggtgaag atccacacgt
    11281 cgggatgccg cagcagcacg tcgcagatcg gccgcagctc ttccgccgaa taggcagcgc
    11341 cggtcgggtt gcacgggttg ttcagcatga accacttggt gcgcggcgtg atcgcctgct
    11401 ccagcgcctc ggcgcgcagc ttgaagccat ggttctgctc gcacggcacc agcaccggct
    11461 tgccatccgc gagcgacacg atgtccggat agctgaccca gcacgggctg gggatgatga
    11521 cctcgtcatc cttgttcaag gtggcgagca tggcgttgaa gatgacctgc ttgccgccgg
    11581 tcgagatgat gatctcttcc ggcttgtagt cgatgccgct gtcccgcttg aactttgccg
    11641 cgactgcctt gcgcaactcc ggcgtgccgg cgacgtccgt gtagtgagtc tcgttcgctt
    11701 cgatcgcgcg gatcgcagcg tccttgacgt tgcgcggcgt gtcgaagtcg ggctcgcctt
    11761 gcgagagcga gacgacattc ttgccttccg cgatcatgtg ccgcgccttg gtcgcgatgg
    11821 cgatcgtcag gctcggttgg atacggtcga ggcgttcggc agtcagttgc atggcggaat
    11881 cctggcagga aacagggcgc gcaacctatc ggaggcgggt cggaggggca acgggcttga
    11941 ccggtggtct gggttgccgg gagcgcggcg gtatgggcgg gattgcccgc ctgtgctgcg
    12001 gcgttccaga gtggcggcaa cggcgccaaa tggttggaag catgtccatc gtcccgatcc
    12061 gcggcctgtt cgaggcgcat ctgacggtca gcgacctgga tcgctcgatc gcgttctatc
    12121 gcgatgtgct ggggttgcct ttggcgcacc ggattccggc gcggaacgtg gcgttcttct
    12181 gggtgccaac atcggacaag gcgatgctgg ggctgtggag catcggcaca tcgccgctgc
    12241 ggctgcggct gcacatcgcg ttcgatgtgg cgctgccgca ggtgttcgag tcggtggcgg
    12301 cgctgcgacg agccgggatc acgccgcgtt ccggcgatgg ggcggcgatc gaggagccga
    12361 tcgtcctggc gtggatgccg gccgccagcg tgtatttcga tgacccggat gggcattcgc
    12421 tggagttcat ctgcatgctg gatgagccgc cgcgcccgga gctggggcag gtttcgtggt
    12481 ctgaatggag gcgtggatga gcgcggagat tcgcgtcgaa cggcgtgagg gctggcgcaa
    12541 gctggtgttc aaccggcccg acaagctgaa cgcggtgaac gaggcgatgc tgcacgcgct
    12601 gatcgctgct ctgaacgagg cggaggcgga gggtgactgc cgcgcggtgc tgttgaccgg
    12661 cgaagggcgc gggttctgca ccgggcagga actggggccg gcggtgatgc cggggccgca
    12721 cggcgcaccc gatctcggcg cggtggcgga actgcatcat gcgctggtgc ggcggttgcg
    12781 agcggtgccg atgccggtgg tgtgcgcggt gaacgggatc gcggccgggg cgggtgccag
    12841 ctacgcgctg gcctgcgaca tcgtgctggc ggcacgcagc gcgcggttca tccaggcgtt
    12901 cgtgaagatc gggctggtgc cggattcggg ggccagcttc ttcctgcctc ggctggtggg
    12961 cgaggcgcgg gcgcgggcac tggcgatgct gggcgatgcg atcgatgccg agcgcgccga
    13021 ggcgtggggc atgatctgga aggtggtgga tgacgcggcg ttgcagactg aggcagaggc
    13081 gctgaccgcg aacctggcgc gccagccgac ggcgtcgctc gcggcgatga agcggatgtt
    13141 cgccgcagcg acggcgaatt cgctggaggc gcagctggac ctggagcgg
//
LOCUS       DATGEM010000634         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_194478, whole genome shotgun sequence.
ACCESSION   DATGEM010000634 DATGEM010000000
VERSION     DATGEM010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194478"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..415)
                     /locus_tag="VKQ27_19525"
     CDS             complement(176..415)
                     /locus_tag="VKQ27_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91184.1"
                     /translation="MTTHVNKVHHVTTITKVAKDLGEDEDWLWDIANEMEIEDGVIWV
                     YGVGEDGVQAFTDFGIDNLIELIKFYKENPGLLKR"
     gene            complement(412..2064)
                     /locus_tag="VKQ27_19530"
     CDS             complement(412..2064)
                     /locus_tag="VKQ27_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046205714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HLY91185.1"
                     /translation="MHADRDAVPDEVAALKKALAVERAKGLEIAGELAVARAKASEDE
                     ALIAQQKLQIAKLKHQIYGQRSERSARLIEQLALTFEELEADATEDELAAERAVAKTT
                     TVRGFTRKRAERQTFPEHLPRERVVIDGPTACECCGSNRLRKLGEDVTRTLEVVPRQW
                     KVIETVREKFSCRDCEKISQAPAPFHAIARGWAGPSLLAMIMFEKFGQHQPLNRQAER
                     YALEGVPIALSTMADAVGAVCASLDPLLRLVEAHVMAAERLHADDTTVPVLAKGKTDT
                     GRCWIYVRDDRPFGGTDPPAAMFYYSRDRKGEHPQAHLARYAGILQADAYDGYNQLYL
                     AGRQPGPIREAACWSHGRRPFFAMADIEENARRKATGKKEIPLSPIAIEVVRRIDALF
                     EIERSINGKSAEERLQVRQTLSRPLVEDLQVYMREQLAKLSRGHDLAKASNYILKRWA
                     SFTLFLEDGRVCLSNNAAERGLRGIALGRKSWLFCGSDRGGRRAAAMYSLIVTAKMNG
                     IDPQAWLTNVLARIAAHPAHRLDELLPWNWTPASALSARAAA"
BASE COUNT          373 a          682 c          648 g          406 t
ORIGIN      
        1 tcattatggc ccgcagcacc gtcttgccgg agatcgcggc ggacgctttc aagatatcga
       61 tcgaagtcac agcgaaacac taagtctaaa gcccgaccgg gtggctacag gttgggttac
      121 gcaggtacac gcaataagcg cgaaccgaag tatccattcg gcgtaggccg caggatcacc
      181 gcttcagcag tccagggttt tccttgtaga acttgataag ctcgatcagg ttgtcgatgc
      241 cgaagtcggt gaacgcctga acgccgtctt ctccgacgcc atagacccag atgacgccat
      301 cctcgatctc catctcgttg gcgatgtccc acagccaatc ttcgtcttcg ccgaggtctt
      361 tggcgacctt ggtgatggtg gtgacgtgat ggaccttgtt gacgtgggtg gtcatgccgc
      421 cgctcgagcg gagagcgcag atgctggcgt ccaattccag ggcagaagtt cgtccagccg
      481 atgagccgga tgagcggcga tgcgggcgag gacattcgtg agccacgcct gcggatcgat
      541 gccgttcatt ttggctgtga cgatcaggct gtacatagct gcggcgcgcc gcccgccgcg
      601 atcagacccg cagaacagcc aggattttcg cccaagtgcg atgcctcgca ggcccctttc
      661 ggcggcattg ttggagaggc acacgcggcc gtcctcgagg aatagggtga agctcgccca
      721 gcgcttcagg atatagttgg acgccttagc caggtcgtgc ccccgagaca gcttggcaag
      781 ctgctcccgc atatagacct ggaggtcctc gaccagaggc cggctcagcg tctgccgcac
      841 ctgaaggcgc tcctcggcac tcttgccgtt gatggaccgt tcgatctcga acagcgcatc
      901 gatccgccgc acgacctcga tcgcgatcgg cgagagcggg atttcctttt tgccggtggc
      961 cttgcgccgg gcattctctt cgatgtcggc catcgcgaag aacgggcgcc gtccatgcga
     1021 ccagcaggca gcctcccgga ttggcccggg ctggcgtccc gccagataga gctggttgta
     1081 cccgtcatag gcgtcggcct gcaggatgcc ggcataccgg gccagatgcg cttggggatg
     1141 ctcgcccttg cggtcgcggg agtagtagaa catcgccgcc ggcgggtccg tgcccccaaa
     1201 tggccgatcg tcgcggacgt agatccagca ccgtccggtg tcggtcttgc ccttggccag
     1261 gaccggtacc gtcgtatcgt cagcatgaag gcgctcggcc gccatgacgt gggcttccac
     1321 caggcgcagc agaggatcca gcgaggcaca gaccgcccca acggcatctg ccatggtcga
     1381 cagtgcgatc ggcacgcctt ccagagcgta gcgctccgcc tggcggttga gcggctgatg
     1441 ctggccgaac ttctcaaaca taatcatggc caagaggctg ggaccagccc acccgcgagc
     1501 gatggcatgg aacggcgctg gcgcctggct gatcttctcg cagtcgcggc aggagaactt
     1561 ctcgcggacc gtctcgatca ccttccactg gcgcggcacc acttctagcg tgcgggtcac
     1621 gtcctcgccg agcttgcgaa gacgattgct gccgcagcac tcacaagccg taggcccatc
     1681 gattaccacc cgttcccgtg ggaggtgctc gggaaaggtc tggcgctcgg cgcgcttgcg
     1741 cgtaaatccg cgcaccgttg tcgtcttggc cacggcccgt tccgccgcca gctcgtcctc
     1801 ggtggcgtca gcttccagct cttcgaacgt cagcgccaac tgctcgatca gccgcgccga
     1861 gcgctccgac cgttgcccat agatctgatg ttttagcttg gcgatttgca gcttctgctg
     1921 cgcgatcaac gcctcgtctt ccgacgcttt cgcgcgggcg accgcgagct cgccggcgat
     1981 ctccaaaccc ttcgcgcgct cgactgccag cgccttcttc agggcggcaa cctcatccgg
     2041 aacagcgtcg cgatcagcat gcatgcgacg cagtgaatca cagattggac gcgatgggac
     2101 tccccaaaa
//
LOCUS       DATGEM010000635         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_911384, whole genome shotgun sequence.
ACCESSION   DATGEM010000635 DATGEM010000000
VERSION     DATGEM010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911384"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VKQ27_19535"
     CDS             <1..396
                     /locus_tag="VKQ27_19535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLY91186.1"
                     /translation="GRKQKGRASYYAHHFDHRKMANGQRFNPNTNVAASKTLPFGTTA
                     RVTNLRNGRSAMVRVEDRGPWIDGRVVDLTPKVAGELGLREAGVAPVVVAPVAVPQKN
                     GEVKLGAGAADASPQEVEQATLEAEAETR"
     gene            complement(437..1255)
                     /locus_tag="VKQ27_19540"
     CDS             complement(437..1255)
                     /locus_tag="VKQ27_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HLY91187.1"
                     /translation="MNNLHRELAPISDAAWAQIEEETTRTLKRYLAGRRVVDLGAPEG
                     VTLSAVGTGHLGGIAAPGDGIIARQREVKAVVELRVAFELDRQQVDDVERGANDSDWQ
                     PAKDAAQKIAFAEDRAIFDGYAAAGITGIRQGTSNPVIALPSDVRDYPDAIAHALSQL
                     RQVGVNGPYSVVLGAGPYTALAEATDNGYPVLQHVRRLVETEIVWAPAIDGAVVLTTR
                     GGDFDLHIGQDISIGYLSHTESSIRLYLQESFTFLLLTAEASVALAPAATKSAV"
     gene            complement(1252..2319)
                     /locus_tag="VKQ27_19545"
     CDS             complement(1252..2319)
                     /locus_tag="VKQ27_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HLY91188.1"
                     /translation="MQQATNPSTAIPQLVTGKLTRAAVFLVVTVNADPAVADTVRSVV
                     SDLDALVRTVGFGQPDGGLTCVVGFGSDVWERLFGVPRPAELHPFREIRTGTRHAVST
                     PGDVLFHIRAERPDLCFELTTLIMNALGDAVAVADEVQGFQYFDRRDLLGFVDGTENP
                     AAQEAIEAALITDSDPAFAGGSYVIVQKYLHDMAGWNSLSTETQERIIGRTKLQNVEL
                     DDAIKPTSAHNALTVIVEDGKEMKIVRDNMPFGQPGRGEFGTYFIGYCQAPRTIEQML
                     ENMFIGRPPGNYDRLLDFSHPVTGTLFFVPSADFLARVADGEIPAAPDGPPATEPSEP
                     PVQPAQDGSLGIGSLKGKPQQ"
     gene            2426..2656
                     /locus_tag="VKQ27_19550"
     CDS             2426..2656
                     /locus_tag="VKQ27_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91189.1"
                     /translation="MGIYLWAEEHLQLHEVITALETDQHGKVVICDSAVEGFLALRPT
                     SQMRIKTLMERGFRKAGDVENRLGYHVGQIGR"
     gene            2759..>3026
                     /locus_tag="VKQ27_19555"
     CDS             2759..>3026
                     /locus_tag="VKQ27_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91190.1"
                     /translation="MTVLLLVGIDSHPARAAEPLAATDEAGWVVQQSELCSSAVQQAE
                     QRYNLPHGLLTAIAKAESGRPVYALTDVRPWPWTIDADGTGLFLD"
BASE COUNT          540 a          923 c         1028 g          535 t
ORIGIN      
        1 gggcggaagc agaaggggcg cgcatcctac tacgcccacc atttcgatca tcggaaaatg
       61 gccaacggcc agcggttcaa ccccaacacg aatgttgccg cgagcaagac actgccgttc
      121 ggcaccacgg cgcgggtgac caacctcagg aatggcaggt cggccatggt gcgggtcgag
      181 gaccgtggtc cgtggatcga tggccgcgtg gtggacttga cgccgaaagt ggccggcgag
      241 ttgggcctcc gcgaggccgg cgtggcgccc gtggtggtcg cgccggtagc ggtgccccag
      301 aagaacggcg aggtgaagct gggcgcgggc gcagccgacg ccagcccgca ggaggtggag
      361 caggcgactc tggaggccga agcggagaca aggtagatag gccgtccggc gcgaacagct
      421 tgcgctgcgc gatctatcaa accgctgatt ttgtggcggc aggcgcgagc gcgaccgagg
      481 cctccgccgt caacagcagg aaggtgaagc tctcctgtag atacaggcgt atcgatgatt
      541 cggtgtggct gagatagccg atggaaatat cctgcccgat gtgcaggtcg aagtcgccgc
      601 cgcgcgtggt cagcacgacc gcgccgtcga tggccggcgc ccagacgatc tccgtctcga
      661 cgagacgccg gacatgctgc aggacgggat aaccgttatc tgtcgcctcg gcgagcgcgg
      721 tgtaaggccc tgcccccagc accacggaat aaggtccgtt gacgcccacc tggcgcaact
      781 ggctcagcgc gtgagcgatg gcatcgggat aatcccgcac gtcacttggc agagcgatga
      841 ccggattgct ggtaccctgg cggatgccgg tgatacccgc cgcggcatag ccgtcgaaga
      901 tcgcgcggtc ctcggcgaac gcgatcttct gggcggcgtc ctttgcgggc tgccagtcgg
      961 aatcgttggc gccgcgctcc acgtcatcga cctgctggcg atccagttcg aacgccacgc
     1021 gcagttcgac cacagccttc acctcgcgct ggcgggcgat gatcccgtcg cccggcgcgg
     1081 cgatgccgcc caggtgaccg gtgcccacgg ctgacagcgt gacgccttca ggcgcgccca
     1141 gatccaccac acgccgcccc gccaggtagc gcttcagtgt gcgggtggtt tcctcttcga
     1201 tctgtgccca tgcggcatcg gagatcggcg caagctcgcg gtgcagattg ttcattgctg
     1261 aggctttcct ttgaggctgc cgatcccaag cgagccatcc tgtgcgggct ggacgggggg
     1321 ttctgaaggt tccgttgcgg gcggtccgtc gggcgccgcc gggatttcgc catccgcaac
     1381 gcgggccagg aagtcggccg agggaacaaa gaacagcgtt ccggtcaccg gatggctgaa
     1441 atcgagcagc cggtcgtagt ttcctggcgg acggccgatg aacatgttct ccagcatctg
     1501 ctcgatcgtc cgcggcgcct ggcagtagcc gatgaagtag gtgccgaact cgccgcggcc
     1561 gggctgaccg aacggcatgt tgtcgcgcac gatcttcatc tccttgccgt cctcgacgat
     1621 gacggtcagc gcattgtggg ccgacgttgg tttgatcgcg tcatcgagtt cgacgttttg
     1681 cagcttcgtg cgcccgataa tacgttcctg cgtttccgtg ctgaggctgt tccagccggc
     1741 catatcgtgc aggtacttct gcacgatcac gtagctgccg ccggcgaaag caggatcact
     1801 gtcggtgatc agagcggcct cgatcgcttc ctgtgccgcg gggttttccg tgccgtcgac
     1861 gaagccaagc aggtctcggc ggtcgaaata ctggaagccc tgcacctcgt ccgcgactgc
     1921 gaccgcatcg ccgagcgcgt tcatgatcag cgtggtgagt tcgaagcaca gatccgggcg
     1981 ctcggcgcgg atgtggaaca gcacgtcgcc tggtgtggag acggcgtggc gcgtgccggt
     2041 gcgtatctcg cggaacggat gcagttccgc gggacgcggc acgccgaaca ggcgttccca
     2101 cacatccgag ccaaagccga cgacgcaggt gagcccaccg tcgggctgcc caaagccgac
     2161 ggtgcggacc agcgcatcga ggtcgctgac aacagaacga acggtgtcgg cgacggctgg
     2221 atcggcattc accgtcacga cgaggaagac cgcagcccgc gtgagcttgc cggttaccag
     2281 ttgcggaatg gctgtggagg ggttcgttgc ctgctgcatc gtgtcaccgg gaagtttgca
     2341 acgaacgagg ctctatcctg gaggttggcg aacggccttg ccactcctgt agccgaacgg
     2401 gaccagcgcc tcaagcagcg ggaccgtggg catatatctt tgggccgaag aacatttgca
     2461 attgcatgag gtcatcacgg cgctggaaac tgaccaacac ggcaaggtgg tcatatgcga
     2521 cagcgcggtt gagggcttct tggctctccg tccgaccagc cagatgagga taaagacgct
     2581 gatggaacgc ggtttccgca aggctggaga tgtcgaaaat cggctgggct atcatgtcgg
     2641 gcagatcggt cgttgaaccc ggttggcgga gtgaccgcgc cgccgtaagc ttttggcggt
     2701 gacagctctg atcaatcgag gggcgggaaa cgtccgggca atatggcctg tgatcggctt
     2761 gaccgttctg ctgctggtcg gcatcgacag ccacccggct cgcgccgcgg agccgttggc
     2821 ggcgaccgat gaagccggct gggtggtgca gcagagcgag ctctgttcat ccgcggtcca
     2881 gcaggccgaa caacggtata atctgccgca cggacttttg accgccattg ccaaggccga
     2941 gagcggccgc ccggtctacg ccctgactga tgtgcgtccc tggccatgga ccatcgatgc
     3001 cgatggcacc ggactgttcc tcgaca
//
LOCUS       DATGEM010000636         7625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1070508, whole genome shotgun sequence.
ACCESSION   DATGEM010000636 DATGEM010000000
VERSION     DATGEM010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070508"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VKQ27_19560"
     CDS             complement(<1..589)
                     /locus_tag="VKQ27_19560"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLY91191.1"
                     /translation="MATSLLLGLIGAGIQASRAPALHEGEAAEHGLRCIYKLIDLEQL
                     KLGTEALPELLAAAERMGFAGVNITHPCKQAVIPLLTGLSPDARAIGAVNTVLLRDGK
                     RIGNNTDWYGFAESFRQGLPDVSMSRVVQLGAGGAGSAVAYAAAKLGVRRLTIFDIDA
                     ARAGALAGQICAQFGAERAVAGTDIEAAVAAADGLI"
     gene            681..2561
                     /locus_tag="VKQ27_19565"
     CDS             681..2561
                     /locus_tag="VKQ27_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLY91192.1"
                     /translation="MPPRQYPKSIATVSLSGTLPEKLEAAAAIGFDGIEIFENDLLTF
                     DGSPEDVRAIAEGLGLTITIFQPFRDFEAMPDPQRTRNLDRAERKFDVMQALGTDLVL
                     VCSNTHAATLDDDARAAADLAEMAERAKRRSLRVGYEALAWGRHVNRWRRAWKIVQAA
                     DHEALGLIVDSFHTLAVDDDAAGIADVPAEKLFFVQLADAPKLAMDSLSWSRHFRNFP
                     GQGQLPVADFLRSVLDAGYQGPLSLEIFNDEFRAAPARLIARDGLRSLLLVESEAGAT
                     TLPPPPRFDGIEFIEFAVDDEAARRLGEQLRDLGLHYVGRHRSKAVDLFRQGRVNFVL
                     NSELDSAASEHFQLHGTSVCAMALRVDDAQQALARARALLCPEWQERVGEGERRIPAL
                     RAPDGTLIYLVQPHPSGRSIFEDDFKLFADASDEATLTGIDHVAQALPVGRMDNFVLF
                     YRAVLGFGPEQLWEIADPYGLVRSRTMVSPDRSIRLPLNVSESRETGTGRFLTTYAGA
                     GVHHIAMATGDIIQTMRRLVARGAHTLPIPANYYEDVAAKWGLDEARIEELRSLNLLY
                     DCDDNGEFLHAYTDAFDDRFFFEIVQRIGGYQQYGAVNAAVRMAAQAQRYPGARMAGL
                     LA"
     gene            2642..3886
                     /locus_tag="VKQ27_19570"
     CDS             2642..3886
                     /locus_tag="VKQ27_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLY91193.1"
                     /translation="MTARNSGLVVPRRRALLGAAAIAATPLAKPFIRSAAAADTIKIG
                     LLLAKTGDIAAQTEYLANGTYMALEERGNKIMGQPAELVWLDEPSPQGSQQNMQKLVQ
                     ENKVVAILGGSLSSNALAEQATAAQLKIPFVCNNAAATAMNGANCNHYTFRLNTPVAV
                     QAAMFAPFIMGYGKKWYFLTANYAFGQDIAKSFKEILAKAGGTIVGDDVVPLNTPDYS
                     SFILKIRQAKPDMILGGVPAGDLSTFLKQWNEMGMKGKIPICEIAIGDTDIWAVGEQA
                     ATGLYTSLWWYDNPNNTADEKKMAAAYQKKYGKPAADKAWMGWIAARSLFESIDAVKA
                     TDPEAIIKGLESWKGGTAANPYSWRKGDHQMLLRNLVVEVKPKVTDKWDYFDVKGVVP
                     KDVADLDKVFGDPAANGCHMST"
     gene            3894..4763
                     /locus_tag="VKQ27_19575"
     CDS             3894..4763
                     /locus_tag="VKQ27_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLY91194.1"
                     /translation="MLDVILSQVATGLVLGGLYVLIAIGLSIIFGLLGIVNFAHGNFF
                     ALGAYFALTLYQYLGWPGVLLSPILVGLVGMAVERTLIRQLYDKEPLISLIITFALAL
                     MIEALLRLIYGGIGQPFNQPEFLSGIYLWGPLLITKYRLTVFLTTVVLLIALWAFMQF
                     TPYGRILRAGSRDPEMVGLLGIRLPRVLTAVFGLGCCLAGIAGLLAAPLWTVIPSMSE
                     NAIMPAFVVVVIGGLGSFAGAVIAGLLVGVVIALTIQFEPDAANAMMYVFMAAVLLVR
                     PRGLLGERWERFE"
     gene            4760..7282
                     /locus_tag="VKQ27_19580"
     CDS             4760..7282
                     /locus_tag="VKQ27_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLY91195.1"
                     /translation="MTAVLRHPILWVLLVLMALTIAWDAIGAPVSLITQIAIYTLYGA
                     GVNLLVGYTGLVPFGASVFFGCASYAVAVSLRGFLHNEAEALIFAVAFSLVLAAAIAL
                     IILRRRGLYFSLLTLAFSQIAFEVAYKWTDLTGGENGLQGVPRPWLVSDWSFHVFTVV
                     TVTVGMWLLWRLAHTPFGRALQALRDNEQRMQSLGYSTFRLKFEAFVIMGGVIGYSGA
                     LLALMLRGAYADNLGWQHAGDALLMTVLGGVHQFLGPLWGAIAFTLLQDRLSAITESW
                     WLMFAPIIILFALLSPEGIQGAVQRLLRREQRWTLVRSSIPPRPDRIAPFRDAQRRVM
                     DADKPILTVRGVHKRFGSLIVASDIHLDVHPYRLHSLIGPNGAGKTTFFNMLTGLLRV
                     DAGTITFDGHSITQLPIHRRIRLGLGRSFQILSVFRMLTTFENVRVAVQARSPQQNNL
                     WRDAYIVEEINARTWSLLAAVGLEDRAAESCANLSHGEQRLLEIAIALATDAKLLLLD
                     EPLAGLAEADREVVGALIRQLATTHAVLLIEHDIDRVLALSDRITVLHQGRVIADGKP
                     AEVASNPDVITAYMGTARAGGVPVPTVRPEVAQPISSKPILALAQVRAGYAGSRILSD
                     VDLVVHEGEAVALLGRNGVGKTTMLRAITGTVEKSAGSITLEGTDITRAKPYEINRLG
                     ISIVPEGRRLFPNLTVMENLRLAARPGGASFEEIFGLFPKLKILQRARAESLSGGERQ
                     MLAIARALTVPSKLILLDEPFEGLAPSVVQEVMDALVRLRGRVAMIIVEHHAETVLPI
                     VDRAYVLVNGQIAYEGSARLLEADHALQARLLGVVQPELTAA"
     gene            complement(7287..7412)
                     /locus_tag="VKQ27_19585"
     CDS             complement(7287..7412)
                     /locus_tag="VKQ27_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91196.1"
                     /translation="MATQASNGTTPLHLVLAWGFVGIPLAWGVVQTVVNALKLFQ"
     gene            complement(7422..>7625)
                     /locus_tag="VKQ27_19590"
     CDS             complement(7422..>7625)
                     /locus_tag="VKQ27_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91197.1"
                     /translation="AGIFGPVLVNYIRQYNVEHGVAKADAYDVTMYVMSALLIVGFVC
                     NLFVKAVEERHYMPHEEADAMGM"
BASE COUNT         1361 a         2455 c         2364 g         1445 t
ORIGIN      
        1 tgatcagccc gtcggccgcc gcaaccgcgg cctcgatgtc ggtcccggcc accgcgcgct
       61 cagcgccgaa ctgcgcgcag atctggcctg ccaaggcgcc cgcgcgtgcg gcatcgatat
      121 cgaagatggt cagccgtcgc acaccaagct tggccgctgc ataggccacc gccgaacccg
      181 caccgcccgc acccagttgc acaacgcggc tcatcgacac gtccggcaac ccttgtcgaa
      241 agctctccgc gaacccatac cagtccgtgt tgttgccgat gcgcttgccg tcccgcagca
      301 gcaccgtgtt caccgcgccg attgcgcgtg catcgggcga aagtcccgtc agcagcggga
      361 taaccgcctg cttgcacgga tgcgtgatgt tcacgcccgc gaaccccatg cgctccgccg
      421 ccgccagtag ctcaggcaac gcctcggtgc ccagcttcag ttgctccagg tcaatcagtt
      481 tgtagatgca gcgcagcccg tgttcggcag cctcgccctc gtgcagtgcc ggcgcccgcg
      541 acgcctggat gcccgcgccg atcaggccaa gaagcagact ggttgccatg cgaacgtccc
      601 gatgtgttgt atttgtgcga gcacccatct gcggctagta gtaccgcgtg tcaacgcagc
      661 agctggcagg agatccgacg atgccgccac ggcaatatcc caagtcgatc gccacggtct
      721 cgctcagcgg caccttgcct gagaagcttg aagcggccgc tgccatcggc ttcgacggca
      781 tcgaaatatt cgagaatgac ctgctgacct tcgatggctc gcctgaggac gtgcgcgcga
      841 tcgccgaagg gcttggcctc acgatcacga tcttccagcc attccgcgat ttcgaggcga
      901 tgcccgaccc acagcgcacc cgcaacctgg atcgcgcgga acgcaagttc gacgtgatgc
      961 aggcgctcgg caccgatctt gtgctggtgt gcagcaatac ccacgccgcg acacttgacg
     1021 atgacgcccg tgccgcggcg gatctggccg agatggcgga acgggcgaaa cgccgaagcc
     1081 tgcgcgtcgg ttacgaagcc ctcgcatggg ggcgtcacgt caatcgatgg cgacgcgcct
     1141 ggaagatcgt gcaggcggca gaccatgagg cgctcggttt gatcgtcgat agttttcata
     1201 cgctcgcggt ggacgacgac gcagccggca tcgcggacgt accggcggag aaattgttct
     1261 tcgtgcaact tgccgatgcg ccgaagctcg caatggattc gctgtcgtgg agccggcatt
     1321 tccgcaactt ccccggccaa ggccaattgc cggttgccga tttcctgcgt tcggtattag
     1381 acgcaggcta tcagggtccg ctctcgcttg agatattcaa cgacgaattc cgtgccgcac
     1441 ctgcccggtt gatcgcgcgc gacggcctgc ggtcgctgtt gctggtcgag tccgaggcgg
     1501 gtgccacgac gctgccgcct ccgcccagat tcgacggcat cgagttcatc gaattcgccg
     1561 tggacgatga ggccgcgcga cgattgggcg agcagttgcg cgacctcggc ctgcattacg
     1621 tcggccggca tcgttccaag gcggtggacc tgttccgcca gggccgcgtc aacttcgtgc
     1681 tgaacagcga gttggacagc gccgcctcgg agcatttcca gctgcacggc acctcggtct
     1741 gcgccatggc gctacgggtc gacgacgcac aacaggcact cgcgcgtgcg cgcgcgctgc
     1801 tttgcccgga atggcaggaa cgagtgggcg aaggcgaacg ccgcattcca gctcttcgcg
     1861 ccccggacgg cacgctgatc tatctggtgc agccgcatcc ttccggccgc agcatcttcg
     1921 aggatgattt caagctgttc gccgatgcgt cggatgaagc cacgctgacc ggcatcgacc
     1981 atgtcgcgca ggcgctgccg gtcgggcgta tggacaactt cgtgctgttc taccgggcgg
     2041 tgcttggctt cggccccgag cagctctggg agatcgccga tccctatggg ctggtgcgca
     2101 gtcgcaccat ggtcagcccg gaccgcagca tccggttgcc gttgaacgtg tcggaaagcc
     2161 gcgagaccgg cacggggcga ttcctcacca cctatgccgg cgccggcgtg caccatatcg
     2221 cgatggcgac tggcgatatc atccagacga tgcgccggct tgtcgcgcgt ggtgcccaca
     2281 ccttaccgat accggccaac tattatgagg acgtggcggc aaaatggggc ctggacgaag
     2341 cacggatcga ggaactgcgc tcactcaacc tgctgtacga ttgcgacgac aatggagaat
     2401 tcctgcacgc ctacacggac gccttcgacg accgtttctt cttcgagatc gttcagcgca
     2461 tcggcggata ccagcaatat ggggcggtga atgccgccgt gcggatggcc gcccaggcac
     2521 agcgttatcc aggcgcgcgt atggccgggt tgcttgccta gcgccgtcgc ggtaagcggg
     2581 cgatcccagc gtgacaatta cgctgggcac ccaaaccggg tgacaggttg agggaggttc
     2641 catgactgcc agaaattcgg gcctggttgt gccgcgccgt cgtgcgctgc tcggtgccgc
     2701 ggcgatcgct gccacgccac tggccaagcc attcattcgc agcgccgcag ctgccgacac
     2761 gatcaagatc ggcctgctgt tggccaaaac cggcgacatc gccgcacaga ccgaatatct
     2821 ggcgaacggc acctacatgg cgctcgagga acgcggcaac aaaatcatgg gccaacctgc
     2881 ggaactggta tggctcgatg agccttcacc acaaggctcg cagcagaaca tgcagaagct
     2941 ggtgcaggag aataaggtcg tcgcgatcct cggcggctcg ctcagttcca acgcactggc
     3001 cgagcaggca acagccgctc aattgaaaat tccgttcgtc tgcaacaatg ctgcggcgac
     3061 ggcgatgaac ggtgccaact gcaaccatta caccttccgg ttgaacaccc ccgtggccgt
     3121 gcaagccgcg atgttcgcgc cgttcatcat gggctatggc aagaagtggt acttcctgac
     3181 tgccaactac gcgttcggcc aggacatcgc gaagtccttc aaggaaatcc tggcaaaggc
     3241 cggcggcacc atcgttggcg acgacgtcgt accgttgaac acgcctgact acagctcctt
     3301 catcctgaag atccgccagg ccaagcccga tatgatcctg ggtggcgtcc ccgccggcga
     3361 cctgtcaacg ttcctgaagc agtggaacga gatgggaatg aagggcaaga tccccatctg
     3421 cgagatcgcc atcggcgaca ccgacatttg ggcggtcggc gagcaagcgg ccaccggcct
     3481 ctacacgtcg ctgtggtggt acgacaatcc caacaatacc gccgacgaaa agaagatggc
     3541 ggccgcctac cagaagaagt acggcaagcc ggcagccgac aaggcctgga tgggctggat
     3601 cgcagcaaga tcgctgttcg aatcgatcga tgcggtgaag gcgacggatc ccgaagcgat
     3661 catcaagggc ctcgaaagct ggaaaggcgg caccgctgcc aatccatact cctggcgcaa
     3721 aggcgaccac cagatgctgc tgcgcaacct ggtggtcgag gtgaagccga aagtcaccga
     3781 caagtgggac tatttcgacg tgaagggcgt ggtgccaaaa gacgtggccg acctcgacaa
     3841 ggtgttcggg gatccggccg cgaacggctg ccatatgtcg acctgatcgc atcgtgctcg
     3901 acgttatact ttcgcaggtg gccaccgggc tcgtgctcgg tggcctctac gtcctgatcg
     3961 ccatcggcct gtcgatcatc ttcgggctgc tcggcatcgt gaacttcgcg cacggcaatt
     4021 tctttgccct gggcgcatat ttcgccctga ctctgtatca gtatctcggt tggcctggcg
     4081 tgctcctgtc ccccatcctg gtgggcctcg tcggcatggc ggtcgaacga acgctcatcc
     4141 gccaactcta tgacaaggaa ccactgatca gtctgatcat caccttcgct cttgcgctga
     4201 tgatcgaggc gctgctgcgc ctgatttacg gcggcatcgg acagccgttc aaccagccgg
     4261 agttcctgtc gggcatctat ctgtggggcc cgctgctgat caccaagtat cggctgaccg
     4321 tattcctgac cacggtcgtg ctgctgatcg cactctgggc gttcatgcag ttcacgccgt
     4381 atggccgcat cctgcgcgcc ggttcgcgtg atcctgaaat ggtgggattg ctgggcatcc
     4441 gcttgccacg cgtgctgaca gctgtgttcg ggctcgggtg ctgtcttgcc ggcatcgcgg
     4501 gtctcctggc ggcgccgttg tggacagtca tcccatcgat gtccgagaac gccatcatgc
     4561 cagccttcgt cgtggtcgtg atcggcggcc ttggttcgtt tgccggagcg gtgatcgccg
     4621 gcctgctggt tggcgtggtc atcgcgctca ccatccaatt cgagcccgat gcggccaacg
     4681 ccatgatgta cgtgttcatg gcggcggtac tgctggtgcg tccccgtggc ttgctgggcg
     4741 agcgctggga gcggttcgaa tgaccgccgt tcttcgccat cccatcctgt gggtgctgct
     4801 cgtcctgatg gcgttgacca ttgcctggga cgccatcggt gcgcccgtca gcctgatcac
     4861 gcagattgcg atctatacgt tgtatggcgc cggggtaaac ctgcttgtcg gctataccgg
     4921 gctggtgccg ttcggagcct cggtgttctt cggttgcgcc agctacgcag tagccgtctc
     4981 gctgcgcggc tttctgcaca acgaggccga agcactgatc ttcgcagtcg cgttctcact
     5041 cgtgttggct gcagccatcg cactgatcat cctgcgccga cgcggcttgt atttctcctt
     5101 gctgacactg gcattttcgc agatcgcctt cgaggttgcc tacaagtgga ccgatctcac
     5161 cggcggcgag aacggactgc agggcgtgcc gcgcccctgg ctggtgagcg actggtcatt
     5221 ccacgtcttc accgtggtca ccgtcaccgt cgggatgtgg ctgctctggc ggctcgcgca
     5281 cacgccgttc ggccgcgcgt tgcaggcact gcgcgataac gaacagcgca tgcaaagcct
     5341 cggctactcc acgttccgcc tgaagttcga ggcgttcgtc atcatgggcg gcgtcatcgg
     5401 ctattccggc gcactgctgg cactgatgct gcgtggcgcc tacgccgaca atctcggctg
     5461 gcagcatgcc ggcgacgcgc tgctgatgac cgtgcttggc ggcgtgcacc aattccttgg
     5521 tccactttgg ggcgccatcg ccttcacctt gttgcaggac cggctgagcg cgatcaccga
     5581 aagctggtgg ttgatgttcg ccccgatcat aatcctgttc gcgctgctgt cgccggaagg
     5641 catccaggga gcggtgcagc gcctgttgcg ccgggagcaa cgctggacat tggtgcgctc
     5701 gagcattccg cctcgcccgg accgcatcgc gccattccgg gatgcacagc ggcgcgttat
     5761 ggatgccgac aagccgatac tgacggtgcg tggcgtgcac aagcggttcg gttcgctcat
     5821 cgtcgcttcg gatatacacc ttgatgtgca tccctaccgg ctgcacagcc tgatcggacc
     5881 gaacggcgcg gggaaaacga cattcttcaa catgttgacc ggcctgctgc gcgtcgatgc
     5941 gggcacgatc acattcgacg gtcacagcat cacgcagctt cccatacatc gccgcatccg
     6001 cctggggctc ggccgctctt tccagatcct gagcgtgttc cgcatgctta ccacattcga
     6061 gaacgtgcgc gtcgcggtgc aggcccgcag cccgcagcag aacaacctgt ggcgcgatgc
     6121 ctatatagtc gaggagatca atgcccgcac ctggtcgctt ctggccgcag tcggcctgga
     6181 ggatcgcgcg gcggaatcgt gtgccaatct gtcgcacggc gaacagcggc tgctcgagat
     6241 tgccattgcg ctcgcaaccg acgcgaagct gctgttgctg gacgagccgc tcgccggatt
     6301 ggcggaagca gaccgcgagg tggtcggcgc gttgatccgc cagctcgcca cgacacatgc
     6361 cgtgctgctg atcgaacatg atatcgatcg cgtgctcgcg ctgtcggatc gcatcaccgt
     6421 gctgcaccag gggcgcgtca ttgccgatgg caagccagcc gaggtcgcaa gcaaccccga
     6481 tgtgatcacc gcctatatgg gcacggcacg cgccggtggc gtgccggtgc caacggttcg
     6541 ccccgaggtg gcccagccga tctccagcaa gccgatactg gcgttggcgc aggttcgcgc
     6601 cggctatgcg ggcagccgga tcctcagcga cgtcgacctc gtcgtgcacg aaggcgaggc
     6661 tgtcgcgctg ctcgggcgca acggcgtcgg caagacgacg atgctgcgcg ccattaccgg
     6721 gacggtggag aaaagcgcag gcagcatcac gctagaaggc accgacatca cgcgcgccaa
     6781 gccgtacgag atcaaccggc ttggcatttc gatcgtaccg gaaggccgcc gcctcttccc
     6841 caacctgacg gtgatggaga acctccggct tgctgcgcgt cccggcggcg catcattcga
     6901 ggagattttc gggcttttcc cgaaactgaa gattctccaa cgggcacggg cggaaagcct
     6961 gtccggtggc gaacgacaga tgctggcgat cgcccgcgca ttgaccgtgc caagtaagtt
     7021 gatcctgctg gacgaaccgt tcgaaggatt ggcgccatct gtggtgcagg aggtcatgga
     7081 cgcactggtc cgcctgcgcg gccgcgtcgc catgatcatc gtcgagcacc acgccgaaac
     7141 ggtgctgccg atcgtcgatc gcgcctatgt cctggtgaat gggcagatcg cctacgaagg
     7201 cagcgcaagg ctactggaag cggaccacgc gttgcaggcg cggctgctcg gcgtggtcca
     7261 gccggagttg acggcggcct gaacggctac tggaacaact tcagcgcatt gaccactgtc
     7321 tggaccacgc cccacgccag gggaatcccg acaaaccccc aagccagcac cagatgcagc
     7381 ggcgtcgtgc cgttgcttgc ctgtgttgcc atcacgccct cctacattcc cattgcatcg
     7441 gcttcctcgt gcggcatgta gtgccgttcc tcgaccgcct tgacaaacag gttgcagacg
     7501 aagccaacga tcagcaatgc cgacatgacg tacatcgtca cgtcgtaggc atccgccttc
     7561 gccacgccgt gctccacgtt gtattggcgg atgtagttca ccagaaccgg gccgaagatg
     7621 ccggc
//
LOCUS       DATGEM010000637         6966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_503159, whole genome shotgun sequence.
ACCESSION   DATGEM010000637 DATGEM010000000
VERSION     DATGEM010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503159"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VKQ27_19595"
     CDS             <1..561
                     /locus_tag="VKQ27_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91198.1"
                     /translation="RKLHAARVPRRRWDGVMIRLASFGLLIGATALSAILAWEVGVFA
                     PAQPPVASPPHAVVAAAAVPAVAPDRTNEWVNAILARPLFSRDRRPAAGGTASATAVS
                     SLPRLSGVLVGPFGRRAIFAADGSKPVVVGEGAQVSDWTVRVIEAGAVRLMGPGGEQV
                     LRPTFAQSDGSTGLVPPQRTGAARPR"
     gene            660..2867
                     /gene="gspD"
                     /locus_tag="VKQ27_19600"
     CDS             660..2867
                     /gene="gspD"
                     /locus_tag="VKQ27_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HLY91199.1"
                     /translation="MLPALPPATEGAAPRVNGTVGSPKGLPPAEFSYGTPQQPASAVG
                     PSASGAADISLDFADTDIRAVVTQILGTILKVNYTIDPSVHGSATLHTDTPLMRSQLL
                     PVLQSLLAQNGAALVQSGALYRVVPIAAAAAVAAESGTAGTEPVTLRYASADDLAKVL
                     QPFVGPEGKIAADPGRNILLVSGAPDTRENLIRLVQSFDIDVLAGQSYALLPVESGAA
                     KDFAAALEDAFQGQKGGSLAGHVRVVPMDRINAVLAIAARPQAIEQVRRVYSLVERDR
                     ARTVRSWHVYYLQNSHAEDVAYVLQQAFTPNNVTAQPPAQRRTSSGVAGLQGSGGVLG
                     ASAGQGGFGGAGQGGGMLGAAGGGGLAGGIGQLGSSPLAQAPQAAAQAAPSLPAAAAP
                     LAGGLSAGSGEAPGQTLSIIPDPQNNALLVYGTGQESETVLAMLRKIDILPLQVRIDA
                     VIAEVTLNDNMQYGTQFFFRSGGLNGILSFASQQNVQSPAQAQLNLGFPGFFLGSHGQ
                     GGAPFALNALQQVTTVRVLSSPQLMVLDNQPARLQVGNVVPYLSQTSQSTITSNAPVV
                     NSINYQQTGVVLDVTPRVNSGGLVTMDISQEVSDVSPNVTTQGINSPTFFDRSISSRV
                     VVQDGQTVGLAGLISDNTSTGNQGIPWLKDIPLLGALAGNQNNTRQRTELLMLLTPHV
                     LHDQRDARALTEDMREQLINAAAVPLQLNGLPPSGSPDPNARIIRKVQQGLDQ"
     gene            complement(2885..3334)
                     /gene="gspG"
                     /locus_tag="VKQ27_19605"
     CDS             complement(2885..3334)
                     /gene="gspG"
                     /locus_tag="VKQ27_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HLY91200.1"
                     /translation="MSKLSRLLRDLRRPAADEAGFTLLEMLVVIAILGLLITLVAPAA
                     LRQLSSARNSVAHQSIARLGSVLDMYKLDVGTYPTTAQGLDALVDKPTDVSTWNGPYL
                     QGGKTPLDAWNHPYVYRSPSSRPDHDYDLCSEGPTGNANADAMICNN"
     gene            3409..3822
                     /locus_tag="VKQ27_19610"
     CDS             3409..3822
                     /locus_tag="VKQ27_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLY91201.1"
                     /translation="MTIPFLACMASVAAFYHLPPRVLPSIQAVEGGYPGAIHVNTNGT
                     WDLGVMQVNTTWVQPLARQTGLSPPVAQQRLLYDPCFNIAVAGAIVRIFLDEAHGDLL
                     RAVGYYASHTPPLAEAYRLRVIGSAVRLFGNSRSR"
     gene            3819..4229
                     /locus_tag="VKQ27_19615"
     CDS             3819..4229
                     /locus_tag="VKQ27_19615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLY91202.1"
                     /translation="MKRDREYGFTLLEVVIAFVIAVIALGVMFKVAIGSLQASRVSAR
                     YEEAVVRARSHLAMATHGGSVMPGEWSGDDGGGYQWHIHVTPLARTAVTSGGGPSTSL
                     ALYAVSVWITWSEGSSTREVRLDTEQIGQAFGSG"
     gene            complement(4233..5900)
                     /locus_tag="VKQ27_19620"
     CDS             complement(4233..5900)
                     /locus_tag="VKQ27_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLY91203.1"
                     /translation="MRLLVERGHCDARAIERARQVAVQTGRQPEAVLIQLGLLTERGL
                     AETYAELLGLPIAQVSRYALEAPLLAECLPARFLREARSLPVAVDDDVVVLATVDPLD
                     AFTPAAVAAAAGRRVVLEVAVPIELESALERLYPTEAASTEDPDMATTNTPLEDDTER
                     LKDLASEAPVIRLVNQILTRAVETNASDVHFEPFEDRFRVRYRHDGVLHEAENPPHRL
                     AAAITSRIKIMARLDIAERRLPQDGRIRLAVRGQEIDFRVSTAPSLHGETVVMRILDR
                     NAVTFDYAKLGIPPDVVRQFREALALPNGIVLVTGPTGSGKTTTLYTGLLELNSIMRK
                     IITVEDPIEYRLHGITQVQVHEQIGLTFAEFVSTVVRQDANVIMVGEIRNQETAQVAT
                     QAALLGRLVLSTLHTNSAAAAVTRLRDMGMEDYLLAAVLRGILAQRLVRRLCMACKRP
                     APAPPELIERFDLAALGKDGPLTLHHAVGCAECRNTGYHGRFAIAEFLSLTADIERLI
                     FQRADHGSIERAAVASGMVTLFQCGLRAALDGETTIEEVARTVQSAV"
     gene            complement(6047..6247)
                     /locus_tag="VKQ27_19625"
     CDS             complement(6047..6247)
                     /locus_tag="VKQ27_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91204.1"
                     /translation="MRVANLGAMDIRAGLTIVATAAICYSAWLTSIVFCAANDDRPLL
                     IASAAFFPVGIVHGIGVWFGGW"
     gene            complement(6432..>6966)
                     /locus_tag="VKQ27_19630"
     CDS             complement(6432..>6966)
                     /locus_tag="VKQ27_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91205.1"
                     /translation="VLLARAASGRDIDFIERRTHTPEPEPAPADAEAPQPTPSATAPK
                     RIARPSRRMGWNDPEFFMPTLEDLERQVRRRPVGRTILDICLDLAVVPGFCQSAFWNE
                     LFDIMNWFGGSVDRLMREKTRRRQAFGQEQDRTPGSTWDWLHLQRDTIRRILGFFIGE
                     VPINPLDPATAIATAPP"
BASE COUNT         1185 a         2136 c         2447 g         1198 t
ORIGIN      
        1 cgcaagcttc acgctgctcg cgttccgcgc cgccggtggg acggggtcat gatccgtctg
       61 gccagcttcg ggctcctgat cggcgccaca gccctttccg cgatcctggc gtgggaggtt
      121 ggggtgttcg ccccggctca accgcccgtg gcgtcgccac cacacgcggt ggtcgcggcc
      181 gcggcggttc ccgcggttgc tccggaccgc acgaacgagt gggtcaatgc catcctcgcg
      241 cgcccgctgt tcagtcgtga tcgcaggcca gccgccggcg gcacggcgtc ggcaacggca
      301 gtcagcagct tgccgcggtt gtccggtgtc ctggtcgggc catttggccg ccgcgccatc
      361 ttcgcggccg acggcagcaa gcccgtggtg gtgggagaag gcgcccaggt ctccgactgg
      421 accgttcggg tgattgaagc aggtgccgtg cgattgatgg gcccaggtgg ggagcaggta
      481 ttgcgcccaa cctttgcgca atcggatgga agtacgggcc ttgtgccacc ccagcgaaca
      541 ggcgcagcac gaccgcgatg atgcaacgag gtcactcagg tcacgtccgg cgcgccgtcg
      601 ccgtcctggc tgttgcctgg ctcgcgggct gccagcacgc ggaggaaccc acggtggcga
      661 tgctgccggc tctgccgcca gctacagaag gcgccgcgcc gcgcgtcaat ggcactgttg
      721 gctcgccgaa ggggcttccg ccggcggagt tctcctatgg cacgccgcag caaccggcga
      781 gtgccgtcgg tccgtcggcc agcggtgccg ccgacatctc gctggacttt gccgacaccg
      841 acattcgtgc cgtggttacc cagatcctgg ggacgatcct gaaggtcaac tatacgatcg
      901 acccatcggt gcacggttcg gcgacgctgc acacggacac gccgctgatg cggtcgcaac
      961 tgcttcctgt gctgcagtcg ctgctggcgc agaatggcgc ggcactggtg cagtccggag
     1021 cactctaccg cgtcgtgccg atcgcggccg ctgccgcggt tgcggctgaa agcgggacag
     1081 ccggcacgga gccggtgacc ttgcgctatg cctcggccga tgacctggca aaggtgctgc
     1141 agccatttgt cggtcccgag ggcaagatcg cggccgatcc tggccgcaac atcctgctgg
     1201 tgagcggcgc gccggatacc cgggagaacc tgatcaggct ggtccagtcg ttcgatatcg
     1261 acgtgctggc gggccagtcc tacgcgttgc tgccggtgga gagtggcgcc gccaaggatt
     1321 ttgccgccgc gctggaggac gcattccagg ggcagaaggg cggctcgctg gcaggccacg
     1381 tgcgcgtcgt gccgatggat cggatcaatg cggtcctggc gatcgccgcg cggcctcagg
     1441 cgatcgagca ggtgcgccgg gtttattcgc tggtcgagcg tgaccgcgcc cggacggtgc
     1501 ggagctggca tgtctactac ctgcagaaca gccacgccga ggacgtggcg tatgtgctgc
     1561 agcaggcgtt cacgccgaac aacgtgacgg cgcagccacc cgctcagcgc aggacgtcga
     1621 gcggcgtggc gggtctgcag ggctcggggg gtgtgctggg cgcgtctgcc gggcagggcg
     1681 ggttcggcgg ggctggtcaa ggcgggggta tgctgggtgc cgcgggcgga ggaggactgg
     1741 cgggcggcat cgggcaactc ggctcatcac cgctggctca ggcgccgcag gccgcggcac
     1801 aggcggcacc gtcgctgcca gccgccgcgg caccgctcgc cggagggctc tccgccggca
     1861 gcggggaagc gcctggacaa acgctcagca tcattccgga tccgcagaac aacgcattgc
     1921 tggtctatgg caccggccag gagagcgaga cggtcctggc gatgctgcgg aaaatagata
     1981 tattgccact gcaggttcga atagacgccg ttattgcaga agtaacgctg aacgataaca
     2041 tgcaatacgg cacgcagttc ttcttcaggt ccggcggcct caacggcatc ctgagcttcg
     2101 catcgcagca gaacgtgcag tcgccggctc aggcgcagct caaccttggg ttcccgggct
     2161 tcttccttgg cagtcacggg caaggcggcg cgccctttgc gctgaatgcg ctgcagcagg
     2221 tgaccacggt gcgcgtgctg tcatcgccgc agctgatggt gctggacaac cagccggcgc
     2281 gactgcaggt cggcaatgtc gtgccctacc tgagccagac ttcgcagagc acgatcacgt
     2341 cgaacgcgcc ggtggtgaac tcgatcaact accagcagac cggcgtggtg ctggacgtca
     2401 cgccgcgggt gaactcgggc ggcctcgtga cgatggatat ttcgcaggag gtgagtgacg
     2461 tatcgcccaa tgtcaccaca caggggatca attcaccgac gttctttgat cgcagcatca
     2521 gctcgcgcgt tgtggtgcag gacgggcaga cggtcggtct ggcggggctg atcagcgaca
     2581 atacgtcgac cggcaaccag ggcatcccct ggctgaagga cattccgctc ctgggtgcgc
     2641 tggcagggaa ccagaacaac acgcgccagc gcactgagtt gctgatgctg ctgacgccgc
     2701 atgtgctgca cgatcagcgc gatgcgcgcg cactgaccga ggatatgcgc gagcagctga
     2761 tcaatgccgc ggcggtgccg ctgcaactga acgggctgcc gccgtccgga tcacccgatc
     2821 cgaatgcacg gatcattcgc aaggtgcagc agggcctgga ccagtgagtg cgcgaacggc
     2881 gcgcctagtt gttacagatc atcgcgtcgg cgtttgcatt gccagtcgga ccttcggagc
     2941 aaaggtcgta atcgtggtcc ggccggctgg acggcgagcg gtacacgtag gggtggttcc
     3001 aggcatcgag cggcgtcttg ccaccttgca ggtaggggcc gttccaggtc gaaacatcgg
     3061 ttggtttgtc gaccagtgcg tccaacccct gcgcggtggt tggataggtg ccgacgtcga
     3121 gcttgtacat gtccaggacc gaacccaggc gcgcaatgga ctgatgggcg acagagttgc
     3181 gggcgctgct caactggcgc agcgcggccg gcgccacgag ggttatcagc agaccaagga
     3241 tcgcgataac gacgagcatc tcgagaaggg tgaagccggc ttcgtcagcg gcggggcgcc
     3301 gaaggtcgcg cagcaggcga gagagcttgg acatgcgata tacagtcagc cgtggtgcaa
     3361 aagggaacgt cacgccggat caatagccac acttcacgga tagcgtccat gacaataccg
     3421 tttctggcct gcatggcgag cgtggctgcc ttctaccacc tgccgccacg cgtgctgcca
     3481 tcgatccagg cggtggaggg aggctatccc ggcgctatcc acgtcaacac caacggcacg
     3541 tgggaccttg gcgtcatgca ggtgaacacc acctgggtgc agccgctggc gcggcagacc
     3601 ggcctgtcgc caccggtggc acaacagcgc ctgctgtacg atccctgctt caacatcgca
     3661 gttgccggcg cgatcgtgcg gattttcctg gacgaggcac acggcgatct gttgcgtgcc
     3721 gtgggctact acgcgtctca tacaccgccg ctggcggagg cctaccggct gcgcgtgatc
     3781 ggttccgcag tcaggctgtt cggcaacagt cgatcgcgat gaagcgcgac cgcgaatacg
     3841 ggttcacgct gctggaggtg gtgatcgcgt tcgtcattgc cgtgatcgcg ctcggcgtga
     3901 tgttcaaggt cgcgatcggt tccttgcagg catcccgggt ctcggcccgc tacgaggagg
     3961 ccgtggtgag agcccgctcg cacctggcaa tggcgacaca tggcggatcg gtgatgccgg
     4021 gcgaatggag tggtgatgat ggcggcggct accaatggca tatccacgtc acgccgctgg
     4081 cacgtactgc cgtgacgtca ggcggtggcc catcgacgtc cttggcgctc tacgctgtca
     4141 gcgtctggat cacctggagc gaaggcagtt ctacccgcga ggtgcggttg gacacggaac
     4201 agatcggcca agcgttcggg agcggatagc gctcatacag ctgattgtac ggtgcgagcg
     4261 acttcttcga ttgtcgtctc gccatccagt gcggcgcgaa gtccgcactg aaacagcgtg
     4321 accatgccgg acgcgacggc agcgcgctct atgcttccat gatccgcgcg ctgaaagatc
     4381 aggcgctcga tgtcggcggt cagcgacagg aattcggcga tggcgaagcg gccatgatag
     4441 ccggtgttgc ggcattcagc gcagccaaca gcgtggtgca gggtgagggg gccgtccttg
     4501 ccgagagcgg caaggtcgaa tcgttcgatg agttcaggcg gggctggtgc aggacgcttg
     4561 caggccatgc acaggcggcg cacgagacgt tgtgcgagaa tgccgcgcag gaccgcggca
     4621 agcaggtagt cctccatgcc catgtcgcgc agccgggtca ccgcggcggc ggcggagttg
     4681 gtgtgcagcg tcgacagcac gaggcggccg agcagcgcgg cctgtgtcgc gacctgcgct
     4741 gtctcctgat tgcggatctc gcccaccatg atgacgttcg catcctggcg caccacggtg
     4801 ctgacgaatt ccgcgaatgt cagtccgatc tgctcgtgca cctgcacctg ggtgatgccg
     4861 tgcaggcgat actcgatggg atcctcaacc gtgatgatct tccgcatgat ggaattgagc
     4921 tcgagcagtc cggtataaag cgtcgtggtc ttgccgcttc cggttgggcc ggtgaccagc
     4981 acgatgccgt tcggcagggc gagcgcctca cggaactggc gaaccacgtc gggcggtatg
     5041 cccagcttgg cgtagtcgaa tgtgacggcg ttgcgatcga ggatgcgcat gacgactgtt
     5101 tcgccatgca gcgacggtgc ggtggagacg cggaagtcga tctcctgccc gcgcaccgcc
     5161 agacggatgc gcccatcctg cggcaggcgc cgctcggcga tatcgagccg cgccatgatc
     5221 ttgatgcgcg aggtgatcgc cgcggccagg cgatgcggcg ggttctccgc ctcgtgcaac
     5281 accccgtcat ggcgatagcg caccctgaag cggtcctcga aaggctcgaa atgcacatcg
     5341 gacgcgttgg tctcgaccgc ccgggtgagg atctggttga ccagccggat caccggggcc
     5401 tcgctggcga ggtccttcag acgctcggta tcgtcttcca gtggtgtatt ggtggtagcc
     5461 atgtccggat cttccgtgga cgcggcctcg gttggataaa gacgctcgag cgctgactcc
     5521 agttcgatcg gaacggcgac ttccaaaacc acgcgccggc cagcggcggc cgcgacggcg
     5581 gcgggcgtga acgcatcgag gggatcaacc gtcgccagca caaccacatc atcgtcaacc
     5641 gctaccggca gcgatcttgc ttcgcgcagg aagcgcgccg gcaggcactc tgcaagcagg
     5701 ggcgcttcca gcgcatagcg cgagacctgc gcgatcggca gaccgaggag ctcggcatag
     5761 gtctcggcca agcctcgttc ggtcagcagg ccaagctgga tcagaacagc ttccggttgc
     5821 ctgccggtct gcaccgcgac ctgtctggcg cgctcgatcg cgcgggcatc gcaatgaccg
     5881 cgctcgacca ggaggcgcat cagcgattca gacgtgtcct gttcggtggc gacaagcaac
     5941 gcgcatgaac ctttggaacc gggcactcag gtttagcgca ctacccgtcg gggtggaaca
     6001 actgccagcg tcgtgatcga cggcgcgtgc gatgaagcgg cggatattac cagccgccga
     6061 accagacgcc gatgccgtgc acgatgccga cggggaagaa cgcggcggac gcgatcagca
     6121 gtgggcggtc gtcgttcgcg gcgcagaaga cgatagatgt gagccaggcc gagtagcaga
     6181 tcgccgcggt ggcgacgatt gtcagccccg ctcgaatgtc catggcgccc aaatttgcga
     6241 cacgcatcgg ccttctccct gtccgctgac tgtgcggctg ggggcattat ggatcgggca
     6301 gggtgcggaa gccactacct gatgcggacg gacggcggcg tgttttgccc ggtacggcca
     6361 ggatcgaccg gacggctggc tgtcgtcgat gaggtttggc gttggtcaac gccgcggcgt
     6421 tgcgcgccag gtcagggtgg ggcggtggcg atggctgtcg cggggtcgag cgggttgatc
     6481 ggcacctcgc cgatgaaaaa gccgaggatc cggcgaatgg tatcccgttg cagatgcaac
     6541 caatcccagg tgctgccggg cgtgcggtcc tgctcctgac caaatgcctg ccggcggcgg
     6601 gtcttttccc gcatcaatct gtcaacgctg ccgccgaacc agttcatgat gtcgaacaac
     6661 tcgttccaga aggcgctctg gcagaagcca gggacgacag cgagatcgag gcagatgtcg
     6721 aggatggtgc ggccgaccgg gcggcgccgc acctggcgct ccaggtcttc gagagtgggc
     6781 atgaagaatt cgggatcgtt ccaacccatg cggcgggatg ggcgggcgat gcgcttgggc
     6841 gcagtggcgg atggagtggg ctgcggcgct tcggcatccg ccggggcggg ctcgggttcc
     6901 ggcgtatggg ttcggcgctc gatgaagtcg atgtcgcggc cggaggcggc gcgggcgagc
     6961 agcacg
//
LOCUS       DATGEM010000638         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1252566, whole genome shotgun sequence.
ACCESSION   DATGEM010000638 DATGEM010000000
VERSION     DATGEM010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252566"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VKQ27_19635"
     CDS             <1..390
                     /locus_tag="VKQ27_19635"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLY91206.1"
                     /translation="RKFGPLVGRILLALIFIISGFGKITGFAGAVGYIQSAGLPAAQL
                     GAIAAIIVELAGGIMLVVGWKARWAAAALFVFIFLAGIFFHAFWASPPEQVMMQQIQF
                     MKNLAIMGGMLYIVVYGSGPLSLDRRP"
     gene            complement(505..1668)
                     /locus_tag="VKQ27_19640"
     CDS             complement(505..1668)
                     /locus_tag="VKQ27_19640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91207.1"
                     /translation="MLTMLIALCAGVFLVTSAGASMAPFLNLISRDLSTTLPAVAHLF
                     SLQAITWGIASLIAGMIADRLGRRMILVGGIFLMGAMRLGFTTSDSYGAAVTWQVLSG
                     IGGGCFMGLVYAVVSEHSAPEIRGRAMSWVITGQSLSLVLGVPLVTLLGTLGGWRGAI
                     ATHGACVLLCAIAVRLATPADPPSHAHAQRPKPPYALLFKPKLMALLAAGTTERLCFA
                     IVAIFLPAYLQHAYDTAFGTLALILGLVAAGNLIGNIIGGRIADRTRSRPRVFAIGSA
                     LTAIVALPLLTWHPAIATSVALGFVYSLVNAAGRPSLMAALAEVPSEIRSALFGLNIT
                     MASLGWLMAGSVGGSLIAAGGFFGLGLFASGVAALGCGLALFSAAMTARAGAR"
     gene            complement(1810..3327)
                     /locus_tag="VKQ27_19645"
     CDS             complement(1810..3327)
                     /locus_tag="VKQ27_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLY91208.1"
                     /translation="MAVQYPTPEQIWDAAEEVGLALTEDDVKSYLGLIKPNIDAYNMV
                     DSMPDYLPAVKYPRTPGYFPDPSENPRNAWYVRTSIKGASSGPLKGKKVAVKDNVMVA
                     GVPMMNGNSILEGYVPEIDATVVARLLDAGAEIVGKTHCESYCISGGSHTGAKGPVHN
                     PHKMGFSAGGSSSGSGVAVSLGEADLAIGCDQGGSIRMPSSWCGIYGMKPTHGLVPYT
                     GIMPIEIYVDHTGPMTGTVTDNALMLEVIAGPDGYDPRQYGAPKKVKYSKLLDGGVKD
                     LKIGMVKEGFGRPESEAASDAKVKKAAELFKKLGAKVEEVSIPAHLIGPALWTPIGVE
                     GITQTMMFGDGYGLSRPDLYMTSMMDKLHGWELRANELSETTKVLTTLGVYIKKYHGS
                     HYYGKAMNVTKRLIEAYDASLAQYDLLLMPTMPMKAQPLPAPGCSREEYFQRALEMIG
                     NTCPFDITHHPAMTVPCGFVDGLPIGMMLIGKHHDEATIYRAAYAFEQSGDWRKM"
BASE COUNT          643 a         1038 c         1037 g          656 t
ORIGIN      
        1 agaaagttcg gaccgctggt ggggcgcatt ttgttggcct tgattttcat catctcgggt
       61 ttcggaaaaa taaccggatt cgccggagcc gtcggctaca tccagtcggc gggcttgccc
      121 gccgcgcaac tgggcgcgat cgccgccatc atcgtcgagc tcgccggcgg catcatgctg
      181 gtggtgggct ggaaggcgcg ctgggcggct gcggccctgt tcgttttcat tttcctggcc
      241 gggatcttct ttcacgcgtt ctgggcttcc ccgcccgagc aggtcatgat gcagcagatc
      301 cagttcatga agaatctggc gatcatgggc ggcatgcttt acattgtcgt ttacggttcc
      361 ggaccgctca gcttggatag acgaccctga cattccgttt gcaaaaaacg acgcggcttc
      421 gggctgcgtc tttttttgca acagagaggg tggaagtgaa gaggtgaaag gggaaagtga
      481 tctgaagctt ttactttttc ccttttacct cgcgccggcg cgcgcggtca ttgccgcact
      541 gaacagtgcc agtccgcaac ccagcgccgc gacgcccgag gcgaacagtc ccaacccgaa
      601 aaacccaccg gctgcgatca gcgagccgcc gaccgagccc gccatcaacc agccgagact
      661 ggccatggtg atgttaaggc cgaacagcgc gctgcggatt tcgctcggca cttcggccag
      721 cgcagccatc agagacgggc gtcccgcggc attcaccagg gaatagacga atcccagcgc
      781 caccgaggtc gcgatggcgg gatgccaggt gagcaacggc agcgcgacga ttgcggtcag
      841 cgcggagccg atggcgaaga cgcgcggccg ggaacgcgtg cggtcggcga tgcgcccgcc
      901 gatgatgttg ccgatcaggt tgccggccgc gaccaaaccg agaatgagcg ccaatgtgcc
      961 aaaggcagtg tcataagcgt gctgcaggta agcaggcaga aatatcgcta cgatcgcgaa
     1021 gcacaggcgt tcggtggtgc cggccgcgag cagtgccatc agcttcggtt tgaacagcag
     1081 ggcgtagggc ggtttcgggc gctgcgcgtg cgcatgcgaa ggtggatcgg cgggtgtggc
     1141 cagccgcacg gcaatcgcgc acaacagcac gcaggcaccg tgtgtcgcga tcgcgccgcg
     1201 ccagccgccg agcgtcccca gcagcgtgac cagcggcacg ccaagaacca gggacagtga
     1261 ctggccggtg atgacccagc tcatcgcgcg tccgcggatt tccggcgcgg agtgctcgga
     1321 caccacggca tagaccaatc ccatgaagca accgccgccg atgccgctca acacctgcca
     1381 tgtcaccgcc gcgccatagc tgtccgacgt cgtgaatccc agccgcatcg cccccatcag
     1441 gaaaatgccg cccaccagga tcatgcgccg cccgaggcga tccgcgatca tgccggcaat
     1501 cagcgaggcg atcccccagg tgatcgcctg aagggaaaac agatgcgcga ccgctggcaa
     1561 cgtggtcgac agatcgcggc tgatcaggtt cagaaacggc gccatgcttg ccccggccga
     1621 tgtgacgagg aagacccccg cgcacaacgc gatcagcatc gtcagcattt ggcgggaggt
     1681 cataaaggca gtgactggtg attggcgact ggtaaaagca agaagggaac cgcgagagcg
     1741 attcccttcc ggcattctat aaccaatcta gacttcttta atcaccaatc accaatcacc
     1801 aatcattcat cacattttcc tccaatcccc cgactgttcg aacgcatacg ccgcgcgata
     1861 gatggtcgct tcgtcgtgat gcttgccgat cagcatcatg ccgatcggca ggccatcgac
     1921 gaagccgcag ggcacggtca tggccgggtg atgggtgatg tcgaacgggc aggtgttgcc
     1981 gatcatctcc agcgcgcgct ggaagtattc ctcgcgcgaa cagccgggtg cgggcagggg
     2041 ttgcgctttc atcggcattg tcggcatcaa cagcaggtcg tattgcgcca acgaggcatc
     2101 gtaggcttcg atcagccgct tggtcacgtt cattgctttg ccgtaatagt ggctgccgtg
     2161 atacttcttg atgtacacgc cgagcgtggt cagcaccttg gtcgtttcgg acaactcgtt
     2221 cgcgcgcagc tcccagccgt gcagtttgtc catcatgctg gtcatgtaga gatccggccg
     2281 gctcaggccg taaccgtcgc cgaacatcat ggtctgcgtg atgccctcga ccccgatcgg
     2341 cgtccatagc gccggcccga ttaggtgggc gggaatggaa acttcctcga ccttggcgcc
     2401 gagcttcttg aatagctcag ccgctttctt caccttcgca tcggaggcgg cctccgattc
     2461 cggccggccg aagccctcct tgaccatgcc gatcttgagg tccttgacgc cgccgtcgag
     2521 cagcttcgag tacttcactt tcttcggtgc cccatattgg cgcgggtcgt atccatccgg
     2581 tcctgcaatc acttcgagca tcagcgcgtt atcggtcacc gtcccggtca tcggcccggt
     2641 gtggtcgaca tagatttcaa tcggcatgat gccggtatag ggcaccagtc cgtgcgtggg
     2701 cttcatgccg tagatgccgc accaggaaga aggcatgcgg atggaaccgc cctggtcgca
     2761 gccgatggcg agatcggctt cgccaagcga taccgcgacg ccgctgcccg aagaggagcc
     2821 gccggcggag aaacccatct tgtgcgggtt gtgcaccggg cccttggcac ccgtgtggct
     2881 gccgccggaa atgcagtagg actcgcaatg cgttttgccg acgatttccg cgcctgcatc
     2941 gagcaggcgc gcgaccacgg tggcgtcgat ctcgggcacg tagccctcga gaatcgagtt
     3001 gccgttcatc atgggcacgc ccgccaccat gacgttatct ttcaccgcga ctttcttgcc
     3061 cttcaacgga cccgacgacg cgcccttgat cgaggtcctc acataccagg cattgcgcgg
     3121 gttctcggac ggatccggga aatagcccgg cgtgcgcgga tacttcaccg ctggcaggta
     3181 atccggcatc gaatcgacca tgttgtaggc gtcaatgttg ggtttgatca gcccgagata
     3241 ggacttcaca tcgtcctcgg tgagggcaag gccgacttct tcagcggcgt cccagatttg
     3301 ttccggcgtc ggatattgca ctgccatgat gatctccttt tatgatgtga acggggttgg
     3361 tcgtgccatg cggt
//
LOCUS       DATGEM010000639         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_912081, whole genome shotgun sequence.
ACCESSION   DATGEM010000639 DATGEM010000000
VERSION     DATGEM010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912081"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VKQ27_19650"
     CDS             complement(<1..512)
                     /locus_tag="VKQ27_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91209.1"
                     /translation="MRRDTESVLPELVAVACFLVLLWASIAVAIKHEYDTALSTAAQT
                     TANLARAFEESTRRTISQIDQILLSARAFYSVQGDKFSFDEWAHTQIVPDSMTAAIGM
                     ADARGHVFADTLPIPPGVSIADRPHFKAQVDPSYDSLFISNPVRGRVSGQATIQFTRK
                     LLGPNGEFAGV"
     gene            1026..1454
                     /locus_tag="VKQ27_19655"
     CDS             1026..1454
                     /locus_tag="VKQ27_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91210.1"
                     /translation="MATLRRAIAAARAGDHQLGRELCASVLFEIQPMLARHRALLRTA
                     VCALLMSQGFKLLTRVAVALSGRRIRIVLSLDGPGRVAPPRCQDEAEETILTVDPRWL
                     SQLSVDNAFLQGWCDSLATGKASHSGRPIVYRARLKPEHA"
     gene            complement(1557..2531)
                     /locus_tag="VKQ27_19660"
     CDS             complement(1557..2531)
                     /locus_tag="VKQ27_19660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HLY91211.1"
                     /translation="MIAALRAVAPGPHVTVQDGGRRGWRRFGVSGCGAMDMPSLAAAN
                     ALVGNQPAAPVLEFAHVGGSWEVLADSMRIAVTGGSFGIAVDGVPLQPWRTHRLQRGQ
                     LFHIKGAADAVWGYLAVAQGFDLTPELGSCSTHLRSGLGGGRLAEGDTLPLRVASVPV
                     MRERQLVPHARANDPIRVVLGPQDGYFRRDEVEAFLAAKYRVTHRSDRMGSWLDGPAL
                     THSGGFNIVSDGLVPGCIQIPGAGMPVVLMMDCQTIGGYPKLATIVSADLPRFAQCRP
                     GTAVSFTMLEIDAAQILYRGYRTALAAIEQSVREMPERTTLPFWLRAT"
     gene            complement(2528..3313)
                     /gene="pxpB"
                     /locus_tag="VKQ27_19665"
     CDS             complement(2528..3313)
                     /gene="pxpB"
                     /locus_tag="VKQ27_19665"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HLY91212.1"
                     /translation="MTILRRPDVDRVAAAASITVPPGYPRFAPVGDAALTVEFGNTVS
                     PELNEQVIALDTALAAAELDGIVETVPSYRSLLVCYEPLEISYHKLVAELRHLLARGL
                     PERMRGSTVWHVPVLYEPPHCIDLPEVAWRSGLTEEQVVALHSSVEYVVYTIGYAPGM
                     PYLGGVPAALHLSRRETPRPRIEAGAVMIGGAQGCIVPTTVPSAWYQLGATPLRPFDV
                     SRSDPFLFRPGDRIRFRRVASIEFDDLSRLSFETLLPYIQAHI"
     gene            complement(3325..>4499)
                     /locus_tag="VKQ27_19670"
     CDS             complement(3325..>4499)
                     /locus_tag="VKQ27_19670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91213.1"
                     /translation="QLVAFRALQGLGAGGVQPIANTIVGDIYTPVERARVQGMLSGVF
                     GVSAILGPSLGAFIVQYGDWPLVFWINLPIGIAAIVMIALFLPEHASVRRRQVDYLGS
                     AFLIIAVTLLMLLAMQGGTLAAGPLLGLLAAFILACVMLALHERATAEPMLPLELWRN
                     RVIVSSNVGSLTTGILMMGVTAYLPTYIQGEMGRGPDVAAIILALMSVVWVLGSTLAG
                     LYLPRATYRRIAGLGALLLIAGAVVLISLTPARGPGWAGVGAVLIGLGMGFCNTTFMI
                     SVQTSVGWQQRGAATSSTMFLRFLGQSLGAALFAAVINASLLSRSPAAASALDQLMDA
                     HWRASQAAGDLVALITATADAMRHAYLLTGMLAIIALLVAITYPARLGPASQQRQQ"
BASE COUNT          872 a         1489 c         1388 g          750 t
ORIGIN      
        1 acacccgcga actcgccgtt cggaccgagc aatttgcggg taaactggat cgtcgcctgg
       61 ccactgacac gtccccggac gggattgctg atgaacagac tatcgtagct gggatccacc
      121 tgcgccttga aatgcggccg atcggcgatg ctgacgcccg gcggtattgg cagggtatcg
      181 gcgaacacgt ggcccctggc atccgccatg ccgattgcag ccgtcatgct atccgggacg
      241 atctgagtgt gcgcccactc gtcgaaactg aacttatcgc cttgcacgct gtaaaaggca
      301 cgcgcgctga gcaggatctg gtcgatctga ctgatcgttc ggcgggtgct ttcctcaaat
      361 gcgcgggcca ggttcgctgt ggtctgagcc gcggtactta aggcggtgtc gtactcgtgc
      421 ttgatcgcca ccgcgatact tgcccacaac agaacgagga agcatgcgac cgccaccagt
      481 tctggcagca ccgattccgt gtcgcggcgc atccgatggc tgtgtgcgaa acgctggacc
      541 gcagcccaga cccacgtggc gacagcaagc ggtatgtgca ccagcgctaa cctcctgcgg
      601 ctcgttccct cgggaccagc ctgacgcgag gacattgtgt tcgcgtttgc aacctgcacc
      661 ttatgtcatc ccgctttgcc acacaccgcc gtcgccgtta ttcggtttga tcggctatcc
      721 ccacggtcgg acgcaaaccc cggatctctg cgaccgccgc gtttgtctca attgatgcta
      781 aacccggtta ccaactcgtt tctctttgtt ttttcgttcc gcatgcccgc cgataccaat
      841 ctgtaacgca ttgttgatga aattgccccc gccgcacgcg gggcggttca ggcgtaagtg
      901 gttctggtag tcgaattaag gcgggccgag tgacgatgat tgaacgactg gatgtgatgt
      961 catccggatc ccgcggactg ggcgcggcac gccgcaccgg ggaaaccgca cgggcggaag
     1021 ctgccatggc gacgctgcgg cgggccatcg ccgcggcccg cgccggggat catcaactgg
     1081 ggcgcgagct ttgcgcctcg gttttgttcg agatccagcc aatgctcgca cggcacagag
     1141 cgctcctgcg gacagcggtc tgcgccctgc tgatgtcgca aggtttcaag ctgttgacac
     1201 gggtcgccgt tgcgttgagc ggtcggcgga tccgcatcgt cctatcgctg gacggtcctg
     1261 gccgcgtcgc gccacctcgc tgtcaggatg aagcggaaga gactatcctc acggttgatc
     1321 ctcgctggtt gtcgcaacta tcggttgaca atgcctttct tcaaggctgg tgcgactcgc
     1381 tcgccaccgg caaggccagt cacagcggca ggccgatagt atatcgggcc cggctcaagc
     1441 ccgagcacgc ctgagactcg cgcagtgtct tcggcggccg ttgatcggcg gggtgccaat
     1501 gtccaatggc accgatgcaa tgtacgcatg gcagtgaaca ggatctgatc ccggccctaa
     1561 gtcgcgcgga gccagaacgg cagtgtggtg cgttcgggca tctctcgcac cgactgttcg
     1621 atcgcagcga gcgccgtgcg ataaccacga tacagaatct gagcggcatc gatctcgagc
     1681 atggtgaagc ttaccgcggt tcccgggcgg cattgcgcga accgcggcag atccgccgac
     1741 acgatcgtcg ccagcttcgg gtagccgccg atcgtctgac aatccatcat cagcacgacc
     1801 ggcattccgg caccggggat ctggatgcat ccaggcacca gcccatccga cacgatgttg
     1861 aagccaccgc tatgcgtcag cgccggcccg tccagccagg atcccatccg gtcgctgcga
     1921 tgcgtcacac gatactttgc cgccaggaat gcctctacct cgtcccgacg gaaatatccg
     1981 tcctgcggcc ccaacacgac ccggatagga tcattggcac gggcatgcgg caccaactgc
     2041 cgctcccgca tcaccggaac gctggccaca cgcagcggca gcgtgtcgcc ctcagccagg
     2101 cgaccgccgc cgagtcctga tcgcagatgg gtcgagcaac tgccaagctc tggtgtcagg
     2161 tcgaacccct gagccacggc gaggtatccc cacaccgcat cggcagcacc tttgatgtgg
     2221 aacaactggc ctcgctgcag ccgatgagtg cgccaaggct gcagcggcac gccgtccaca
     2281 gcgatgccga agcttccacc cgtcaccgcg atccgcatcg agtccgccag cacctcccat
     2341 gaaccgccaa catgggcgaa ttccagcaca ggtgcggcgg gctgatttcc gaccagcgca
     2401 ttggccgcgg ccaacgacgg catgtccatg gcgccacaac ccgagacacc aaagcggcgc
     2461 cacccccgcc gcccgccatc ctggaccgtg acgtgcggtc ctggtgcgac cgcgcgcaac
     2521 gcggcgatca tatgtgggcc tgaatgtaag gcagcagtgt ctcgaagctc aggcgggaca
     2581 agtcgtcgaa ctcaatcgat gcgacacggc ggaagcggat gcggtcgccg ggacggaaca
     2641 ggaagggatc agaccgcgac acatcgaacg ggcgcaacgg tgtggcaccg agttggtacc
     2701 acgcgctcgg cacggtggtt ggaacgatgc agccctgcgc gccgccgatc atgacagccc
     2761 ctgcctcgat gcggggccgt ggtgtctccc gccgcgacaa gtgcagcgcg gctggcacgc
     2821 cgccaagata cggcatgccg ggtgcgtagc cgattgtgta gacgacgtac tccaccgatg
     2881 aatgcagtgc caccacctgc tcttcggtca ggccggagcg ccacgcgacc tcgggcaaat
     2941 cgatgcaatg cggtggctcg tagagcaccg gcacatgcca caccgtcgat ccccgcatgc
     3001 gctcgggcag cccgcgcgcc agtagatggc gcaactctgc cacaagcttg tgataggaga
     3061 tttccagcgg ctcgtaacac accagaaggg aacggtagct cggcacggtt tcgacgatgc
     3121 catccaattc cgccgcggcc aaagcggtat ccagcgcaat gacctgctcg ttcaattctg
     3181 gtgatacggt attgccgaac tccacagtca gcgccgcgtc acccaccggc gcgaagcggg
     3241 gatagcccgg gggcacagtg atcgaagcgg cggctgcgac tctgtcgacg tccgggcggc
     3301 gcagtatcgt caccaagaga tcgcctactg ctgccgctgt tggctggccg gccccagccg
     3361 cgccggatag gtgatcgcga ccagcagggc aatgatcgcg agcatgcctg tcaacaggta
     3421 ggcgtgccgc atggcgtcgg cggtggcggt aatcaatgca accaggtcac cggcggcctg
     3481 cgaagcgcgc cagtgtgcat ccatgagctg atccagcgcg ctggcggcgg cgggcgaccg
     3541 gctgaggaga ctggcgttga tgaccgcggc aaagagtgct gcgccaaggg attggcccag
     3601 gaagcgcagg aacatggtgg acgaggtggc ggcaccgcgt tgctgccacc ccaccgacgt
     3661 ctgcaccgaa atcatgaacg tggtgttgca gaaccccatg ccgaggccaa tcagcacggc
     3721 gccgacgccg gcccagcccg gtccccgcgc cggggtcaaa ctgatcagca ccacggcgcc
     3781 cgcaatcagc aaaagcgcgc ccaggcccgc gatccggcga taggtcgcgc gcggcaggta
     3841 aagtccggcc agggtgcttc ccaacaccca gacaaccgac atcaacgcca gaatgatcgc
     3901 agcgacatcc ggaccgcgtc ccatttcgcc ttgtatataa gtcggcagat acgccgtgac
     3961 gcccatcatc aggatgcctg tcgtcaggct cccgacgttg ctgctgacga tgacgcggtt
     4021 gcgccagagc tcgagtggca acatcggttc cgccgtagcc cgttcgtgca gcgccaacat
     4081 cacgcaggcc aggatgaacg cggcgagcag tcccagcagc ggtcccgcag ccagagtgcc
     4141 gccctgcatc gccagtaaca tcagcaacgt gaccgcgatg atcaggaagg ccgaacccag
     4201 ataatcgacc tgtctccgcc gaacgctcgc gtgttccggc aggaacaggg cgatcatgac
     4261 gatggcggca atcccgatcg gcagattgat ccagaagacc agcggccaat cgccatactg
     4321 tacgatgaac gcgccaagcg aaggtccgag gatcgccgac acgccgaaca cgcctgacag
     4381 catgccctgc acccgcgcgc gctccaccgg cgtgtagatg tcgccgacga tcgtattggc
     4441 gatcggctgc acgccccctg ctccgagtcc ctgcagcgcg cggaacgcca caagctgca
//
LOCUS       DATGEM010000640        22050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1501402, whole genome shotgun sequence.
ACCESSION   DATGEM010000640 DATGEM010000000
VERSION     DATGEM010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 22050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22050
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501402"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VKQ27_19675"
     CDS             <1..258
                     /locus_tag="VKQ27_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91214.1"
                     /translation="SAFRLSRQRSPGWQASLSARNPQLHIAPIQELGQTFGISSESSK
                     MLETTPFADRPNLDVLPSAASRPAEDRRILTDFARDQDRAR"
     gene            637..903
                     /locus_tag="VKQ27_19680"
     CDS             637..903
                     /locus_tag="VKQ27_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91215.1"
                     /translation="MKGFVIAVALGAAMLATSAQAGTIKAYKTETGAQKHCPSDQVVW
                     ANSASTVGAFHLQGSKFYGATKNGAYVCRGEAEAGGWHAAANGQ"
     gene            complement(1240..1560)
                     /locus_tag="VKQ27_19685"
     CDS             complement(1240..1560)
                     /locus_tag="VKQ27_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91216.1"
                     /translation="MKITILAMAATMAIAGTAVAQTNGSSAMTSPSSTTTTTNSPDVA
                     GDGPNSRGATRRSMPEMPSNGATGAASQNGTLSEPGTSATVNGAPASPGQKNGSGKAG
                     GGSK"
     gene            1712..2086
                     /locus_tag="VKQ27_19690"
     CDS             1712..2086
                     /locus_tag="VKQ27_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HLY91217.1"
                     /translation="MDDLELLFDRLACSRFRARFRLGDRERSYLADRGLETVLRHARD
                     FIDRRLAPAAPAHDGKQTPMRGHPAFIAQHATATCCRSCLAKWHGIPAGRELDEDERR
                     YVASVIERWLIQQGFPVRPRSG"
     gene            complement(2046..2552)
                     /locus_tag="VKQ27_19695"
     CDS             complement(2046..2552)
                     /locus_tag="VKQ27_19695"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLY91218.1"
                     /translation="MDAVELPIAEMPNTLERALAYWIEKKSSRLMPSRADIDPIEFKD
                     YLGRVALVNVQEAAPRFKLRLIGSSHFFRKHGPRDGHDIMEVKPKEYALEVARQYEET
                     VARREPTVFRTTLAFHGTAFSYTRLALPLGTDGNSPDMLLAVAMFKTDEQRDFFSRYE
                     DELESLAG"
     gene            2866..3075
                     /locus_tag="VKQ27_19700"
     CDS             2866..3075
                     /locus_tag="VKQ27_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91219.1"
                     /translation="MVGYHLYVLDKLGHIAGRSDFVWQDDAAAIRFAETYPCPHGVEL
                     WAGVRRIGKFESNDVADFPAAPALH"
     gene            complement(3221..3949)
                     /locus_tag="VKQ27_19705"
     CDS             complement(3221..3949)
                     /locus_tag="VKQ27_19705"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLY91220.1"
                     /translation="MNFAMSFDGFDLPILRFLTRFVGGWPLADHLIQAISRSDILTGT
                     AMMALVWFAWFQASADMDEVRRTDRQGWILIVSVGGILTMLLAGLLQYTLTVHRPPIV
                     AGLGLTFPIDADLGPANSGNSFPSVDATLFCALSIGLLRLDRRLGTVAFAWTFIVIEL
                     PQVCLGLLYPSDVLAGAVLGILCMEIFIRLPVWRPALRILAWSRRRPGLFYGLAFLLT
                     EQLAHPFGDLLQWAFALLGRFFLR"
     gene            4210..4566
                     /locus_tag="VKQ27_19710"
     CDS             4210..4566
                     /locus_tag="VKQ27_19710"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HLY91221.1"
                     /translation="MKLARLLAVAVLLGLTAPAMAQTAPQTAPAKPAPAAAAKPAATP
                     APATKDQGSTKSDLLDLNSASADDLKALPGIGDAYSAKIIAGRPYKMKTQLVSKKILP
                     QKTYDGIKDKVIARQQ"
     gene            complement(4717..6603)
                     /locus_tag="VKQ27_19715"
     CDS             complement(4717..6603)
                     /locus_tag="VKQ27_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91222.1"
                     /translation="MLSPLSLTQNRQRRGRSVGIVFGVLMSMSFLIGSEAKAVVAESP
                     NGNHVSLLGKINFAALANGIAAKPPLPDAGSVAGKGVLALQPEAREIPLRTHSKTSGV
                     SLNSLGPVSEMLAPIVPNQLVVGLPFTGFTGLTHFDQRNADQGNQFSDEPPDQAFAVG
                     GGYVLEAVNNAIAIYDAKGNQLAPTVSINRFFNEGSEINRATGERSAAQFSDPRAYYD
                     SASKRWFVFEWATFNTPDGTPLNLSAQFFAVSQTSNPLGNWFLYDFDTTNSDIAGCPC
                     LPDFQRIGFDADGVYVAHNMFQLTGAGNYVTATIYALSKKTLEAGAGSSFIRFSLPND
                     FTVDPTVSPPGNVPASFENGTEYLVEDLANLANDGKANKVRLFALSNTKSLSTASPRL
                     FLGSVDTTTQAYSEPPPAVQPDGPRPYAATLVKAGAALPPVPHLDGGDTRFSALPVFV
                     NNQVWAALNTSVPNPAGGTKVGVAYFAFEMLGGAHGFLATLHRQGLITAPAGTSVLYP
                     AIAMPVSGSGGIGVTVVGPGLYPSTGFVTVHGAAVGGIQITGVGALPDDGFTGYDTAK
                     PYTQGTGRWGDYGAAAVDEKGDLWFANEYIPDTATFPRTALANWGTFITHYHPSSSVG
                     VAAK"
     gene            complement(7186..7911)
                     /locus_tag="VKQ27_19720"
     CDS             complement(7186..7911)
                     /locus_tag="VKQ27_19720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91223.1"
                     /translation="MSLVLLTNCSQYTGPGALAALLQDGHTVACHDVAFADAAARAGF
                     NQQYFAAHALVAQAPEAIRDEVAERWGVPDAVVLNDVHPNAPTDIEAIPPDDFRATFE
                     AVVMVPVRLAQLFLPDMKARRSGAFVFVTSARETRPEPGFAVPTTLRAGATAFAKALA
                     KEAAPFGIQANVVAPNYLYSEMYYPRARFVDDLAGREAIARIVPFGRLGQPEEVGALI
                     AFLASGRSPFTTGQVINFTGGWP"
     gene            complement(8182..8649)
                     /locus_tag="VKQ27_19725"
     CDS             complement(8182..8649)
                     /locus_tag="VKQ27_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91224.1"
                     /translation="MKRWLTFAVISALSTLVLLPDRALSACATVKGQGALKSVTFDGK
                     VAHIKMSATGYVLVYHLEVAGNKVYIVSAFDPAEGHTHPHHVYIQDNQGVKSDYFSEG
                     SNDLCYLVSAKSRPLGITQFLQHLIFSVDSSEYFELALPVNGEYHLDKKAPPP"
     gene            complement(9555..9947)
                     /locus_tag="VKQ27_19730"
     CDS             complement(9555..9947)
                     /locus_tag="VKQ27_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY91225.1"
                     /translation="MDRFTGGCLCGNVRIVAAGPPYRVGLCHCLDCRKHHGALFHASA
                     VFPQEAVTIDGETRDYEGRYFCPRCGSSVFARTADEIEVNLGSLDALDQLIPTYESWI
                     IRRESWLPPFPLTRRYDRDRGATSRLEG"
     gene            complement(10141..10458)
                     /locus_tag="VKQ27_19735"
     CDS             complement(10141..10458)
                     /locus_tag="VKQ27_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91226.1"
                     /translation="MIVPQVTFRIWNIRPVRTKTVYAFMGADLEIGGVESRVCGIQAR
                     HLPGGGTSVNLPCYRDVDGAWRAAVDLPAEVQDALSAAVLGCLVAEGVAVNRAAALNT
                     AAQ"
     gene            complement(10756..10968)
                     /locus_tag="VKQ27_19740"
     CDS             complement(10756..10968)
                     /locus_tag="VKQ27_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91227.1"
                     /translation="MAAMIRSHTSGSIGQASQPCCHKVMVFHGLAIGQDRSTPRPRHR
                     SMYLSGDAETAIAVGVSHGKPVVLEV"
     gene            11259..11462
                     /locus_tag="VKQ27_19745"
     CDS             11259..11462
                     /locus_tag="VKQ27_19745"
                     /inference="COORDINATES: protein motif:HMM:NF039472.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HLY91228.1"
                     /translation="MDEDRLNMEIRKFLKVVGITSPAAQRPSLERMGVETKNSVNVGA
                     FTSMQKQFLEFHRTSVLLKSVRG"
     gene            11475..12395
                     /locus_tag="VKQ27_19750"
     CDS             11475..12395
                     /locus_tag="VKQ27_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLY91229.1"
                     /translation="MVQLYVGVTDRDWFDFLSARRTEEGINFWQPGGQTQFKALQPGE
                     LFLFKLHSPNNFIVGGGVFAHASILPVSLVWEAFGEANGAPSIAEMKRRIAKYRRQPV
                     AEREDYHIGCRVLEQPFFLPRERWIPVPASWSSNIVSGKRFDTESVDGRTLWDALGDS
                     LTGIADTGSAVETLRYGQPTLVRPRLGQGTFRLAVTDTYERRCAVSQERTLPILDAAH
                     IWGYADGGVHEISNGLLLRTDIHKLFDLGYVTVSTDGRFEVSRPLKEDFQNGKHYYAH
                     HGQQIWQPRDPGRRPSQEALTWHREERYLG"
     gene            complement(12752..13501)
                     /locus_tag="VKQ27_19755"
     CDS             complement(12752..13501)
                     /locus_tag="VKQ27_19755"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLY91230.1"
                     /translation="MPKLQGKVAVVTGGSSGMGLATAKRFVDEGAFVYIVGRRQAELE
                     KAVSLIGHNVAAVQGDVSNLADLDRLYAKVAREKGKIDILFAGAGIVDPQPLAETTEE
                     SFDKLFAINTRGLAFTVKKALPLLNDGGAIVVISSIAHAKGIPGFTAYSATKAAVRSF
                     VRTWTAELKDRRIRVNAISPGPIDTPIFEQQAPTKEGADHARAQAAAAVPFGRLGRPE
                     EIASAALFLASDEASFVAGVDLPVDGGMTAI"
     gene            complement(13602..14351)
                     /locus_tag="VKQ27_19760"
     CDS             complement(13602..14351)
                     /locus_tag="VKQ27_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91231.1"
                     /translation="MSRLQNKVAVITGGTSGIGLAAAQRFVAEGAFVYIFARRQEELD
                     RTVASIGRNIAGVPGDVRKLEDLDRLYAKIALDGRKLDVVVANVGAVDSVKLADVTIE
                     SFNHNFDVNARGVLFTVQKSLPLLNNGASVILTSTIASLRGFPGRSAYAASKTALRSY
                     ARTWTMELKDRGIRVNTITPGPCDTPLIDAQVSSPEEAAAVRAKHAANIPLGRMARPE
                     EIAAAMLFLASDDASFVTGSELLVDGGMCSV"
     gene            complement(14399..15184)
                     /locus_tag="VKQ27_19765"
     CDS             complement(14399..15184)
                     /locus_tag="VKQ27_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HLY91232.1"
                     /translation="MALSGRLNRPQSRSWNEAMSRSARLFDLIQALRRHRRAVSAVVL
                     AEELGVSKRTIYRDIETLIALGAPIDGAAGIGYLMKPGFILPPLMFTEDEVEALALSS
                     RWIAQRADPDLVTAAENALAKIAAVLPVPLTVALDNSGLLSASPRSSVEDSIDPAVVR
                     AAIRGQRKLRIKYADEAGRTTERDIWPIALVYFEAVRLLVAWCEMRAGFRHFRSDRIA
                     EVVDTGERYPRSRQALTKAWRDSQSPSACCQKLTHPRCSLLAT"
     gene            complement(15591..15743)
                     /locus_tag="VKQ27_19770"
     CDS             complement(15591..15743)
                     /locus_tag="VKQ27_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91233.1"
                     /translation="MVVVMPMMMVAMMHGSLGIGSGERDDGDGGGETEKQLAHGLLDN
                     RVGAAN"
     gene            16130..16837
                     /locus_tag="VKQ27_19775"
     CDS             16130..16837
                     /locus_tag="VKQ27_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HLY91234.1"
                     /translation="MSAKTEPLVPADQLARESPIRIPNESVEYRAARRALLAQEFELR
                     RQIERVAAMRRALPPGGPVTSDYRFETEDGPSDLAGLFGEKQTLVVYNYMFGPERKRP
                     CPMCTNLLDAWDGNADDIAQRVSLVVVARSPLERLAAVKRERGWKNLRLASALTDDFT
                     RAYAGSPDGGLDSGGPAVFTRRDGMIRHFWSQEMIASDPGQDPRGAPDLAPLWTVLDM
                     TPEGRGADWYPSLEYPA"
     gene            17007..17450
                     /locus_tag="VKQ27_19780"
     CDS             17007..17450
                     /locus_tag="VKQ27_19780"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HLY91235.1"
                     /translation="MSQKWEPLPAAGVDDGTILFDGVCVLCSGWVQFVIERDPTGLFR
                     FVPIQSPYGRMLAQKFGIDAEAPETNVVVVDGMAYFKADAALAVLTKLPGWSWAGISR
                     ITPRSVRNWLYDRVARNRYTLFGRTDTCLMPTPDLARRFRNERPS"
     gene            17454..18584
                     /locus_tag="VKQ27_19785"
     CDS             17454..18584
                     /locus_tag="VKQ27_19785"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HLY91236.1"
                     /translation="MAPKVLVIGGAGAFGRRLVRGLLATTDLEVVVAGRDLARAQSVV
                     DEECVGSRVGRATAVALDTRIATAAQLRDTGAFVVVDAAGPFQRQGYHLAERAIEAGL
                     HYVDLADSREFVSGFHVLDERAQKASVTALTGASSTPALSNAVLDHLTAGWQAVDDVE
                     IAISPGNRAPRGLAVVRSILSYAGKPVRVWENGSWRHRPGWGAPVRKDIRGLGPRWLS
                     LCETPDLDVVPTRFAVRRSVTFNAGLELPVMHLGLFAATIAIRLGLLQSLEPLAETFR
                     RAADLLDGWGTDRGGMVVAVSGVGADGTQVSATWTLLAEAGDGPSIPTLPALAAVRAM
                     ASGELHRPGASVCAGVLPLDAIEREFAPYRITTHVELGTRRE"
     gene            19298..20533
                     /locus_tag="VKQ27_19790"
     CDS             19298..20533
                     /locus_tag="VKQ27_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLY91237.1"
                     /translation="MNTLPSRLEDSTVPRPAFADWLGTTNDVTKRFLSAGRVPGLINM
                     GGGLPAPETYPAAELAAIAQRAILEHPQECLGYGPIDGLIELRDVLAERFSTESLRLG
                     RENVLITTSGMQGLDLLGKVLLEPGGLIAGQYPTYLGALDAWKSRSPTYRNIVLEDPD
                     FEPVAVLAGAQFAYTVPNFSNPTGKLVGLEIRQALVDAAHATGTWLVEDDPYGGLHYD
                     GAPLPRMIELSSRRRRGQIYDGPIVYMGTLSKEIAPGLRIGWVVAAPEMIDALTTAKQ
                     GSDMCTSGVTQRIALGALESGLIERLQPVIVALYRDRRDALCAALAEHLANSFTWEVP
                     VGGMFVWAVARDPSVDTDRLWSVALEAGVCVAPSSVFDASGRNRRALRLNFTLNPPDK
                     LAEGARRLAAAVRTMSKQG"
     gene            complement(20564..20998)
                     /locus_tag="VKQ27_19795"
     CDS             complement(20564..20998)
                     /locus_tag="VKQ27_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91238.1"
                     /translation="MLHRIHGLGIWSPAHVLDLPFNPNSRTRDRRFAAILRLAGVTIV
                     NVHLAHGQALLRRQLRHVAAHLTGAAVIIGDMNVVGPVRLPSFPDVGPRGTTHFAKGI
                     LPFRLDRCLARGLMSTGSAILDRGRSDHWPILVELDGPNRHG"
     gene            21107..21511
                     /locus_tag="VKQ27_19800"
     CDS             21107..21511
                     /locus_tag="VKQ27_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91239.1"
                     /translation="METTITVRNLRPLNGRVYALVDADIVIGDIEITVFGIQARHVPS
                     GGTSIHLPTYRDGDGAWRAAVELPEEIREALCDAILAFMVEEGVAKPRQAAADREDAT
                     ATAAEIPSGQDDFGKRLVARKGSLTADVDLGI"
     gene            21607..21888
                     /locus_tag="VKQ27_19805"
                     /pseudo
     CDS             21607..21888
                     /locus_tag="VKQ27_19805"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4092 a         7013 c         7007 g         3938 t
ORIGIN      
        1 tcggctttcc gcttgagccg ccaaagatcg ccgggctggc aggcttccct atcggcccgc
       61 aatcctcaat tgcacatagc gcccatacaa gagctggggc agacttttgg catcagttcc
      121 gagagttcga agatgctcga aacgacgcca tttgccgacc gcccgaacct tgatgttctc
      181 ccatcagcag catctcgtcc ggccgaagac cgtagaatcc tcaccgattt cgcgcgcgac
      241 caggaccgcg cgcgttaggt aacgcgtcgg ccctcgacgc gccgccgaca gcgatctcag
      301 tgcttgtggt gggtgtagtg attatgggtg cgggcgttcc tatagtgacc gcccttgtgg
      361 cttgatccgt ggccgcccgc atagtggccg ccctgggtag acttcgcgaa ggccggagcg
      421 gcagacgaaa cggcgaacgt cagagcgatc gctacgagta tgtgcttgaa catgaatgct
      481 ccagtcggtc aacgagacgg cacgacggta tccggtgacc ggcgcgcgcg tcaatctgtg
      541 gttataggcg cgtggcgttg ctctatcacc gctttacgaa cgaacccgaa tcggatcaac
      601 atccgggcgc acatttttcc gtggaggatt aagcacatga agggattcgt tatcgcggtc
      661 gcgctcggcg ccgccatgct cgcgacgtcc gcgcaggcgg gcacgatcaa ggcctacaag
      721 acagagaccg gcgcgcagaa gcattgcccg agcgatcagg tagtgtgggc caattctgcc
      781 agcaccgtag gagcgttcca cctgcaaggg tcgaaattct acggcgccac aaagaacggc
      841 gcgtacgttt gccgcggcga ggccgaggcc ggtggctggc acgcagccgc gaacgggcag
      901 tagaggcggc cacaagccga cgatccggcc ctcgcttcca agcggcggct gaatcgtcgg
      961 cttgaactca taaacgcatt gccctgggga caacattgtg tgggcgtttc aatccacatc
     1021 ccatcaccga aatctcggcc gggatgatga gcattgcggc tcctcataag cttgattcgc
     1081 caggccgtcc gccgtcaagc actcacctga aacctgacga ttgccattac cgcccagacc
     1141 gacaccgatt ggccggactc tactcaaagc ggtaaaagcc gccgtcggcc agtggccgtt
     1201 gttgacgtct accaatgtgc gacaacgcga tcccactagt catttgctgc cgccaccggc
     1261 tttgcccgag ccgtttttct ggccgggcga ggcgggcgct ccgttgacag tagcactcgt
     1321 gcccggttcg gacagcgtcc cattctgaga agctgctccg gtcgcgccgt tgctcggcat
     1381 ttccggcatc gaacgacggg tcgctcctct cgaattgggg ccgtcacctg cgacgtccgg
     1441 actgttcgtc gtcgtggtcg tgctggacgg cgaggtcatg gcgctcgaac cgttcgtttg
     1501 agcgacggcg gttcccgcga tagccatggt agcggccatg gcgaggatgg tgatcttcat
     1561 cgtcttctcc gacattgcct tatcgcggga caacctccgg agaagagctt ggttcaggcg
     1621 gaccccggca tttcttgacc taagctcgcg ccggtcacca ttgtcccgga tgccgtgggg
     1681 atcggctcga caaaggacga agcgacgcag gatggatgat ctcgagcttc tgttcgaccg
     1741 cttggcatgc tcgcggttcc gcgcgcgctt ccgccttgga gaccgcgagc ggagctactt
     1801 ggccgacagg ggcctggaaa ccgtcctccg ccatgctcgc gacttcatcg atcggcgcct
     1861 ggcgcccgcg gctccggcgc atgacggcaa gcagacgccg atgcgcgggc atccggcctt
     1921 catcgcgcag cacgcgaccg ccacgtgctg ccgttcatgc ctggcaaagt ggcatggaat
     1981 accggcgggt cgtgaactcg acgaggatga gcgacggtat gtcgcctccg tgatcgaacg
     2041 ctggctcatc cagcaaggct ttccagttcg tcctcgtagc ggctgaagaa atcccgctgt
     2101 tcgtcggtct tgaacatggc gacggcgagc agcatgtcgg gcgagttacc gtccgtgcca
     2161 aggggaagcg cgaggcgcgt gtagctgaac gcggttccgt ggaatgcgag cgtcgttctg
     2221 aagaccgtcg gttcgcggcg agccaccgtc tcttcgtact gacgggccac ttccagggcg
     2281 tactctttgg gcttgacctc catgatgtca tggccgtccc gaggaccgtg tttccggaag
     2341 aaatgggagc tgccgataag ccgaagcttg aatcgcggtg ccgcttcctg gacgttcacc
     2401 aacgccaccc gcccaagata atccttgaat tcgatcggat cgatatccgc tcgcgacggc
     2461 atgagacggg aggatttctt ctctatccaa tatgcaagcg cgcgctcaag tgtattgggc
     2521 atttccgcga tcggcagctc gactgcgtcc accgccacat gcaacgaatt catcaagccc
     2581 tcgccaaatg gttgagtagc ttaatatcgc aggcgaaaac actccgcaat gccgagcaaa
     2641 gtcagacgac aaatgttcgg ccgagaactg tcttcccctg ccgttcgacg ggcggccaga
     2701 ggtttagcat tccttgggcg atttggctct cggtctccgt tcctctctgg gacgcgatcg
     2761 gcatgtgcaa ttcgattgca tgaggtggca ccgatcacca tctgcaaggc ttccggatca
     2821 agtttcccga gtgcagtttt cgatcgtccg ccaggagcca tcgccatggt cgggtatcat
     2881 ctgtacgtgc tcgataagct gggacacatt gcaggccgtt ctgacttcgt ttggcaggac
     2941 gacgcagcgg caatccgctt cgccgaaaca tatccctgcc cccacggtgt ggaactatgg
     3001 gccggagtga ggcgcatcgg taagttcgag tcgaatgatg tcgccgactt tccggccgct
     3061 ccggcgctcc actgatcaaa gtggatcgtc agatcagtcg gaaatcggaa caccccgaac
     3121 agacgagcta gggccgccgt cggcgcagac gcccttgaaa gatggctgag tctccgcttt
     3181 ctcgactcct atgggcgggt gatccatggg ccgagccctt tcagcgtaga aagaaccgcc
     3241 ccagcaaggc aaaggcccat tgcagaaggt caccgaatgg atgcgccaac tgctccgtta
     3301 gcaggaaagc gagcccgtag aacagccccg gtcgacgccg actccaggcg aggatacgca
     3361 gcgccgggcg ccagaccggc aggcggatga agatttccat gcacaggatc ccgagcacgg
     3421 ccccggccag cacgtcgctc ggatagagca gccccagaca aacctgcggc agttcgatca
     3481 cgataaacgt ccaggcaaag gcgaccgtac ccagccgccg atcgagccgc agcaggccga
     3541 tcgagagcgc acagaacagc gtggcatcga cgctcgggaa ggaattgcca gaattcgccg
     3601 gacccagatc ggcgtcgatg ggaaaggtca agcctagtcc ggcgacgatc ggcggccgat
     3661 ggaccgtgag cgtgtattga aggagaccgg ctagtagcat cgtcaagatg ccgccgacac
     3721 tcacaatcaa tatccaaccc tgccggtcgg tccgtcgcac ttcgtccatg tcggccgacg
     3781 cctggaacca ggcgaaccag accaaggcca tcatcgccgt tccggtgaga atgtcggaac
     3841 gggagatcgc ctggatcaag tgatcggcca gcggccaacc tccgacgaaa cgggtcagaa
     3901 aacgcaggat cggcagatcg aaaccatcga acgacatcgc gaagttcaac cccgcgccag
     3961 attgctcgta ttttggttaa cgccggtatc agaatgcggc tccagcgccg agagacaatt
     4021 attccccgtg aagccccccc gcgacaaaag cgaccatgga ggcgatcgct ccggagcagc
     4081 aggacgctgg cggtctgaag aggcggattt cgctcacgtc cgcagtccgt cgcccaaaaa
     4141 ggaagatttc ggcggcgccg accgttctga aactggcgac actccgtcgt ccttcgaaag
     4201 gcgcaaatca tgaagctagc tcgactcctc gcagttgctg tcctgctcgg ccttaccgcg
     4261 cccgcgatgg cacaaacggc accccagact gcgcctgcca agccagctcc ggcagccgcg
     4321 gccaaaccgg cggccactcc ggcgcccgcc accaaggacc agggcagcac gaagtccgac
     4381 ctgctcgacc tcaactccgc aagcgccgac gacctcaaag cgctccccgg gatcggtgac
     4441 gcctattcag ccaaaatcat cgctgggcgg ccgtacaaga tgaagacgca gttggtctcg
     4501 aagaaaattc tccctcagaa gacgtatgac ggcatcaagg acaaggtcat cgccagacag
     4561 cagtaacggg cgagccgggg aagagtgtca gctcctcccc ggatccccat atgcgtgagc
     4621 gttacccacc aggcacaatg ttcggcctag atgcgacgac gctcggcgcg cggactgtgg
     4681 attgcgcagt ccgatcgcga tcgcgtcatc ctgtcttcac ttcgccgcca ccccaaccga
     4741 gctcgacggg tggtagtgcg tgatgaacgt gccccaattc gcgagcgcgg ttcgagggaa
     4801 cgtggcagtg tcaggaatgt attcgttggc gaaccacagg tcgcccttct cgtcgacggc
     4861 cgccgctccg tagtcgcccc accggcccgt gccttgggtg tagggcttgg cggtgtcata
     4921 gccggtaaaa ccgtcgtccg gcaacgcgcc gaccccggtg atctggatgc cgccaacggc
     4981 cgctccatgc acggtgacga agccggtgct tggatacagc cccggtccga ccactgtgac
     5041 gccgatgcca ccgctgccgc tcaccggcat cgcgatcgcc ggatacagga cgctggttcc
     5101 cgcaggggcg gtgatcaaac cctgcctatg caatgtcgcc aggaagccgt gggccccacc
     5161 gagcatctcg aacgcgaagt acgccacgcc gactttcgtg ccgccagctg gattcggcac
     5221 ggaggtgttg agcgccgccc aaacctggtt gttgacgaag accggcaggg cggagaaccg
     5281 cgtgtcgccc ccatccaggt gtgggactgg cggaagagcg gctcccgcct tgacgagggt
     5341 tgccgcataa gggcgcggac cgtccggttg aacggccggt ggcggttcag aataggcttg
     5401 ggtagtcgta tcgaccgatc cgagaaataa ccgaggcgac gcggtcgaga gggatttcgt
     5461 gttgctgagc gcgaaaagcc ggaccttgtt ggctttgccg tcgttcgcga ggttcgccag
     5521 atcctccacc agatactcgg tgccgttttc gaagctcgcg ggaacgttgc ccggcggcga
     5581 caccgtggga tcgacggtga agtcgttcgg caggctgaag cgaatgaacg aagaccctgc
     5641 acccgcttcc aaggtcttct tggaaagggc gtagatggtc gccgtcacgt aattgcccgc
     5701 ccccgtcaac tggaacatgt tgtgggcgac gtaaacgccg tcggcatcga agccgatgcg
     5761 ttggaagtcg gggaggcagg ggcatccggc gatgtcgctg ttcgtcgtgt cgaagtcgta
     5821 gaggaaccag tttccgagcg ggttgctcgt ctggctgacg gcgaagaatt gcgccgacag
     5881 attgagaggg gttccgtcgg gcgtgttgaa ggtggcccat tcgaacacga accagcgctt
     5941 ggaagcgctg tcgtagtagg cgcgcggatc gctgaactgc gccgcgctcc gctctccggt
     6001 ggcgcgattg atctcgctgc cttcgttgaa gaaacggttg atcgacacgg tcggagccaa
     6061 ctggttgccc ttcgcgtcgt agatcgcgat cgcgttgttc actgcctcca gcacgtagcc
     6121 gccgccaacg gcgaacgctt ggtccggcgg ctcgtcgctg aactggttgc cctggtcggc
     6181 gttccgctgg tcaaaatgcg tgaggccggt gaacccggtg aacggaaggc cgacgaccag
     6241 ttggttcggg acgatgggcg ccagcatctc ggagacgggt ccgagactgt tcaaggacac
     6301 cccgctcgtt ttcgagtgcg tgcgcagcgg gatctctcgc gcctcgggct ggagggcgag
     6361 cacgcccttt ccggcaacgg aaccggcgtc cggcaacggt ggtttcgcgg caatgccgtt
     6421 cgccagcgcc gcgaaattta tcttgccgag taaggagacg tggttgccgt tcgggctctc
     6481 ggcgacgacc gctttcgctt cggagccgat caggaacgac atggacatga gaacgccgaa
     6541 aacgatcccg accgatcgac ctcgacgttg gcggttctgg gtaagcgaaa gcggtgacag
     6601 catggaagcc ccctcttgtc tggtttgacg cagaactttc gccgttggag tcaatgctcc
     6661 atcggcgcgg gaaggtcgct gtggtcactg cgtttggcag cgaaagcatt gctgcttctt
     6721 gcgggtggca gagtcaaggt ggcagttaac tttctagcaa ccatagcggg cgcggattgg
     6781 atgtcgtgca gacgccgagg ggtcgagagc gcgggcgatc ttttacctct ccgacaagct
     6841 tcgggctttc ccaacaatgg aaccgggcga cctgcagata tccccggtgt gggaaccacg
     6901 catgaagaga cgcacgcgga ccgtcggcga tggccagcga gcgcattagg ccgatggggg
     6961 ttctatcgct ttggtcgctt ccgcgggctc gcttttgctg ccggtgtccg caccatcgcc
     7021 tttagggccg tccgttgctg ccgacgcacc tccgcagtcg ccgccgtcca gattcccgcg
     7081 gcgcgattgg cccacgacag ccatatgctc ctcggagagc gcttcattgc tgtcggccac
     7141 ggaactctcc catcgaatcg ttcgttttca cctgcatgtt ggcgatcaag gccagccacc
     7201 ggtgaaattg atcacctgcc ccgtagtgaa aggcgacctg ccggaagcca ggaacgcgat
     7261 cagcgcgccg acctcttcgg gttggccgag gcgcccaaac ggcacgatgc gcgcgatcgc
     7321 ctcgcgccct gcgagatcgt ccacgaagcg ggcgcgcggg taatacatct cgctataaag
     7381 gtaattcggg gcgacgacgt tggcctgtat gccgaaaggc gccgcttcct tggcaagtgc
     7441 tttggcgaac gcggtcgctc ccgcacgcag cgtcgtcggc accgcgaagc ccggctccgg
     7501 ccgcgtctcg cgcgccgagg tgacaaagac aaacgcgccg ctacggcgtg ccttcatgtc
     7561 cggcaggaaa agctgtgcaa gccgcaccgg aaccatcacg accgcctcga aagtcgctcg
     7621 gaagtcgtcc ggtgggatcg cctcgatatc ggtcggcgcg ttgggatgga catcgttcag
     7681 cacgacagcg tcaggaacgc cccatcgctc ggcgacttca tcacggatgg cctcgggagc
     7741 ctgtgccacg agcgcatgtg cggcgaaata ttgctgattg aagcctgcac gagccgcggc
     7801 atcggcgaag gcaacgtcgt ggcatgcgac cgtgtggccg tcctgcaaga gcgccgcgag
     7861 ggcgccgggg cctgtgtact ggctgcaatt ggtgaggagg acgagcgaca tcggcgtctc
     7921 cgggataggc cgcgcggcac accgggtcgt cagcggctga gtcggcgcct attatcgacg
     7981 agcctgtcac gatcgccgcc catgtcaatg aacgcgcttt cgatcaaagg gcagcgtcgt
     8041 atgtttcttg tgcagccttc ccgcgatctc ctacatcctc gatttcgaac aaggacaagg
     8101 ccgagcatcg agaaacagcg caagcatgat gacggagacg gacgcattga ttgaacgtcg
     8161 caaaatcaat ccgtgaggtc tctacggcgg aggggccttt ttgtccagat ggtattcgcc
     8221 gttcaccggc aatgctagtt cgaaatattc actactatcg accgaaaata ttaagtgttg
     8281 caaaaattgc gtaatcccca gaggtctact tttcgcgctt accagatagc acaaatcatt
     8341 cgaaccttct gaaaaatagt cagactttac accctgatta tcctgaatat atacatgatg
     8401 cgggtgagta tgcccttcag ctggatcaaa tgcactcact atatatacct tgtttccagc
     8461 cacctccaaa tgatacacca gaacgtagcc agtcgctgac attttaatat gcgcgacctt
     8521 gccgtcgaat gttacagact tcagcgcccc ctgacccttt acagtggcgc aagccgacag
     8581 cgctcggtcc ggtagcaata ccaaggtgga tagagccgag ataaccgcga aagtcagcca
     8641 ccgtttcatc ttgtccctcc agagcaagcg gttcaccgtc tcgtcaggaa taaacagcta
     8701 cgatgagcgc gccacgacta atatatagcc gcgcctttag ttgcctcgcc tccaaataat
     8761 tttccagctc tgaccgacaa aagtggtcaa aaccaacgga aacatcgttc ctggggacta
     8821 ggaccgagtc aatcatttat tcgactgata cggcgatcgc tatttacgag gcaaacttcc
     8881 cctactactc gatcgcgaac gagaaagtat atccagacgc catgaaatct caagtacaaa
     8941 gcttttaagc ttcgcgattt ggagcttaca aaaacacaga attgttggcg gctgtggtga
     9001 gaccggcggt ccaacccgcc tcttggtcgt ggagacgaaa tccttgccca ctgctgaaat
     9061 gctttcccac ccgcgcttgc gcgacccgat gccgagcatc agctgcccga ttcccacggg
     9121 caggccgtcg gtctgggccg cgtccgcctg tcgggtggca ccttgcaatc atcgacacgc
     9181 tgcccgtcgg acgccttgtc gctcagccgt tctttggcgc tcaggcttga ctgcggagcg
     9241 ggaacggtgg gaggtatcgg cggatcgttc cggacctgac tccgctcctc ggcgaccgcc
     9301 atgcccgtta gggaaagtcc caccatgagc gccagagcac atcgtttcat ggcatcctcc
     9361 atcgtctcga tgaaagagct tgaactgccc cgaggggatt tcgcgccccg cttcttgctt
     9421 tcgttttcaa tgacccgctg tcttcgagcg ggtgtcacgg cgatgcggaa cgccctcgag
     9481 tgcaccagct gtccgacgcc gctgcaagat cctctcgtca cttcgcagcg gcgccttcgc
     9541 gccgttcgcg cgacctaccc ctcaaggcga ctcgtggcgc cacgatcgcg gtcgtatcgt
     9601 ctcgtgagcg ggaacggcgg caaccatgat tctcggcgga tgatccagct ttcgtaagtt
     9661 gggatcagtt gatcaagggc atccagggat cccaggttca cttcgatttc atctgcggtg
     9721 cgtgcgaaaa cggacgagcc gcagcgggga cagaaatacc gtccctcgta gtcccttgtt
     9781 tcgccatcta tcgtcaccgc ctcctggggg aacaccgcgg acgcgtgaaa aagggcgcca
     9841 tgatgcttgc ggcagtcgag acagtgacaa aggccgaccc ggtatggagg ccccgccgcc
     9901 acaattcgga cgttgccaca caggcaaccg ccggtgaacc gatccatgct gcgtctcctc
     9961 taacatcaag cggccggaat cgttacagcg aatggcagtt tcattctaat tgcccgccga
    10021 gcgcgccagc tacttcggca gctgcggcta cgatgcggac tgatcgaaaa ccgctctaga
    10081 acaggttgac gccggggcga taagcggcgt ggccccgcga tcccccgact gggcggcttg
    10141 ctattgggca gcggtattca gcgcagccgc gcggttgacc gcgacgccct ccgcaaccaa
    10201 gcaccccaac acggcggccg aaagcgcgtc ctgcacctcg gcgggcaggt cgaccgcggc
    10261 acgccaggcg ccgtcgacat cgcgatagca tggcaggttc acagaagtgc cgccgccggg
    10321 tagatggcga gcttgaatcc cgcagacgcg ggattctaca ccgccgatct caagatcggc
    10381 acccatgaaa gcgtagacgg tcttcgttcg cacgggacga atattccaga ttctgaaagt
    10441 aacttgcggc acgatcacgc ccagacaatg attttattta actatatcta cgaggatcgt
    10501 atcgcaagct ttcctgtcgc gttcgacgtc tgcaaactac tatgttgccg atgccagacc
    10561 gatggctcgt cacgccgccg cggcgaccgg gcatccttgt tatcctggag agcgcctcca
    10621 ggaatgcaag gatgaatgcc cggcacccga tgccgccgct cactcttcga tgaattccgg
    10681 cgacaccgaa gcggtcagcc agacgccgtt atctgcctga aagagctcgt gcccggcttc
    10741 ggccatggcc cccgctcaaa cctccaagac gacgggcttg ccatggctaa cgccgactgc
    10801 gatcgccgtc tcggcgtccc ccgacaggta catgctccgg tggcgcggtc gtggtgttga
    10861 acgatcctgc ccgatcgcca gcccatgaaa gaccatgact ttgtgacagc acggctggga
    10921 cgcctggccg atcgacccgg acgtatggct gcggatcatc gccgccatcg aggcactgcc
    10981 gacgccgcaa acggagcgtc tgagttgagc cgcggggcgc cgtcgtggag gcgggtcgcc
    11041 ccatggcagc cgagcgctga ccagcgccgc acgcagcgcg attaagcggg acaccgatca
    11101 aggtgttaaa ctgcggaacc cagcgaggcg atcggcaggg ctccgacggc cgatatgcgg
    11161 gcccccctaa tcgccgggtg tccccgggag aaacgccgag cgctaccgca ttcggggtag
    11221 cgacggaccg gccttccatc ctggacaggg agagagcgat ggacgaagac cggctcaata
    11281 tggaaatacg caagtttctc aaggtcgtcg gcatcacgtc cccagcagca cagcggccat
    11341 cgctcgagcg gatgggcgtg gagacgaaga actcggtcaa cgtcggcgcc ttcaccagca
    11401 tgcagaagca gttcctggaa tttcatcgga cctccgtcct cctcaagtct gtgcgcggct
    11461 gaggctcgaa ggcgatggtc cagctctacg tcggcgtcac ggatcgtgac tggttcgact
    11521 tcctctcggc caggcggacg gaagaaggca tcaacttctg gcagcccggc ggccagacac
    11581 agttcaaagc gctccagccg ggcgagctgt tcctgttcaa gctccacagc cccaacaact
    11641 tcatcgtcgg tggaggcgtc ttcgcgcacg cgtcgatcct gccggtctcc ttggtgtggg
    11701 aagcgttcgg cgaggcgaac ggtgcgccgt cgatcgcgga gatgaagcga cgcattgcga
    11761 aataccgacg ccaaccggtc gccgagcgcg aggactacca catcggctgc cgcgtcctgg
    11821 agcagccgtt cttcttgccg cgcgaacgct ggatcccggt cccggcgtct tggtcgtcga
    11881 acatcgtttc cgggaagcgg ttcgataccg agagcgtcga cggccgaact ctctgggatg
    11941 ccctggggga cagcctgacc ggcatcgccg acaccggttc ggccgttgag acgctgcgat
    12001 acggtcagcc gacgctggtg cggccgcggc tgggtcaagg gacattccgg ctcgcggtca
    12061 ccgacacgta cgagcgccgg tgcgcggtca gccaggagcg gacgctgccg atcctggacg
    12121 cggcccacat ctgggggtac gcggacggcg gagtccacga aataagcaac ggtctcttgc
    12181 tccggaccga catccacaag ctgttcgacc tcggatacgt caccgtgtcc accgacggaa
    12241 ggttcgaggt cagccgtccg ctcaaggagg atttccagaa cgggaagcac tactacgcgc
    12301 accacggcca acagatctgg cagccgcgcg atcccggacg ccgccccagc caggaagcgc
    12361 tgacctggca tcgcgaggag cgttacctgg ggtagtagca ttcaccgaca ggagcgaggc
    12421 gacagcgacg gcgggcatgg gaaagacatt ccagcgacga gagctccgcg acctcgtctg
    12481 gcccaatccg atgtaagtcg gcgcggggcc ccggcgcgga tcggcatcgc aaatgcgtga
    12541 tctcaaaagc acaggagggg tcacgccgcc gcgccggtgc acaaaatccg catcttcgat
    12601 cggccggtcc agggccgtcg cggtcgctgc acagcccgag atagagaact tctcgaaaac
    12661 caaacaggac atcctctcgc ggaggcgccg ctagccttgc ggcacttact cgccgccctc
    12721 gaacatcgtc gagggcggcg gggcgacagc gtcaaatcgc ggtcatgccg ccatcgactg
    12781 ggagatccac gcctgcaacg aagcttgctt cgtcggaagc aaggaacagg gcggccgacg
    12841 caatctcctc cggacgaccg agccgcccga acgggacagc agcggcggcc tgggcgcggg
    12901 cgtggtcggc cccctccttg gtcggcgcct gctgttcgaa gatcggcgtg tcgatcgggc
    12961 ccgggctgat cgcattgacc cggatgcgac ggtccttcag ctcggccgtc caggtgcgga
    13021 cgaaggaacg gacggcggcc ttggtggcgc tgtaggcggt gaagccgggg atgcccttgg
    13081 cgtgtgcgat cgaggagatg acaacgatcg cgccgccgtc gttcaggagc ggcagcgcct
    13141 tcttcaccgt gaaggccaga ccccgcgtgt tgatcgcaaa gagcttgtcg aagctctcct
    13201 cggtcgtctc ggcgagcggc tggggatcga cgatgcccgc gccagcaaac aggatgtcga
    13261 tcttgccctt ctccctggcg accttggcgt agagccggtc gagatcggcg agattggaca
    13321 cgtcaccctg gaccgcggcg acgttgtggc cgatcagcga tacggccttt tcgagctccg
    13381 cctggcggcg gcccacgatg tagacgaacg cgccctcatc gacgaagcgc ttggcggtgg
    13441 ctaggcccat tccgctgctg ccgcccgtga caacggcgac ttttccttgg agctttggca
    13501 ttgaggttct ctttcaggat ggggacggcc gtatcggcct gtcggggtac ggcccgccgg
    13561 ttgactggcg gcgggcctgg aagcggtcgg tctgaaccgg ttcagaccga gcacatgccg
    13621 ccgtcgacca gcagttcgct gccggtgacg aagctcgcgt cgtcggatgc caggaacagc
    13681 atcgcggcag cgatctcttc cggtcgcgcc atgcggccga gcgggatgtt cgcggcatgt
    13741 ttggcgcgaa cggcagccgc ctcctcgggc gaactgacct gggcgtcgat cagcggcgtg
    13801 tcgcaggggc ccggcgtgat cgtgttgacg cggatgccgc ggtccttcag ctccatcgtc
    13861 caggtccggg cgtaggagcg cagggcggtc ttggacgcgg cgtaggcgct gcgaccgggg
    13921 aacccgcgta aggaggcaat cgtgctggtc aggatgacgg aggcgccgtt gttgagaagc
    13981 ggcagcgact tctgcaccgt gaacagcacg ccccgggcgt tgacgtcgaa attgtggttg
    14041 aagctctcga tagtgacgtc ggcgagcttc acgctgtcga cggctccgac attggcgacc
    14101 acgacgtcga gcttgcggcc atccaaggcg atcttggcgt agagccggtc gaggtcctcg
    14161 agcttgcgga cgtcgccggg cacaccggcg atgttccggc cgatcgacgc gaccgtcctg
    14221 tccagttctt cctggcggcg ggcgaagata tagacgaagg ctccctcagc gacgaaccgc
    14281 tgtgccgccg ccaggccaat tccgctggtg ccgccggtga tcacggcgac cttgttttgt
    14341 agtctcgaca tcactgcttt cctcactcgc tggagtcagg ggcggcgcga gccgcccttc
    14401 acgtcgcaag gaggctgcag cgaggatgcg tcagtttctg gcagcaggct gaagggctct
    14461 gggagtcgcg ccaggccttg gtcagcgctt ggcgggatcg cgggtagcgc tcgcccgtgt
    14521 caacgacctc ggcgatccgg tccgagcgaa aatggcggaa gccagcccgc atctcgcacc
    14581 aggcgaccag cagacggacc gcctcgaaat agacgagcgc gatcggccag atgtcccgct
    14641 ccgtcgtccg gccagcctcg tcggcatact tgatccgcag tttgcgctga ccgcggatgg
    14701 ccgctcgcac cacggcgggg tcgatgctgt cctccacaga cgaccggggc gacgcgctca
    14761 gaagcccaga attgtccagt gccacggtca gcggaaccgg cagcaccgcc gcgatcttgg
    14821 ccagcgcgtt ttccgccgcg gtgacgaggt cgggatcggc gcgctgtgcg atccaacgcg
    14881 agctaagcgc cagcgcctcc acctcatcct cggtgaacat cagcggcggg aggatgaagc
    14941 cgggtttcat caggtagccg atcccggccg cgccgtcgat cggggccccg agggcaatca
    15001 gcgtttcgat gtcccggtag atggtccgct tggagacacc tagctcctcg gccagcacga
    15061 ccgcgctgac tgcgcgtcgg tgacgtcgca acgcctggat caaatcgaac agccgggccg
    15121 agcgtgacat ggcttcattc cagctgcgcg attgcgggcg atttagtcgg ccagatagag
    15181 ccatgtcctg aagagagagt aggcaggctg attgaataag agttattcca ggatggaatg
    15241 atcgcgagcg cgagccatca actaccgttt cccttgcggg aatgaacgac gtcttcgaac
    15301 caaattcctc gtacctgcgt ccgcgtgcag tcgagcttgc gcgccccacg gctatccgcg
    15361 acggttctcc gagatccggt ggcccttggc gacgacagac acgctgcccc ccgccgcctc
    15421 gacctccgcc agtagcgccg ccgtctcagc ctcgctcccc tccgttcgcg cccgtgcgcg
    15481 aataggcgtg gcattccggc aacctcgggc ggggaaatga tgccgtcgac cgtcgtcctc
    15541 gcgatgcctc ttccgcagcc gaatctcggc gcgctatttt tctatggcgg tcagttcgcc
    15601 gcaccaaccc tgttatcgag taaaccatgc gcaagttgct tctcggtctc gccgccgccg
    15661 tcgccgtcgt ctctttcacc gctgccgatg cccaggctgc cgtgcatcat cgccaccatc
    15721 atcatcggca tcaccaccac catcctcatc atcatgtcgc ccaggtcgcc ccgcggtaac
    15781 ctgctcgacg cttgagatcg gactgtcgcc gggcttagag ggccttcgcc cggcgatggc
    15841 caccgagccc cgatcggcga cgcgtcggtc ggggttgcgc cgttgattcg cgctgcaaac
    15901 gcgctggccg ctggactaat gctcggcctc tgacactcgg aatgatgggc tgcacatgcc
    15961 cctaagcggg ctgaggcttt cgcttcgcag tcggtgaagc tcgatcagcg ggcgttggct
    16021 tcatgcccag aaagccgacg ccgacagcgg gtctaaaaaa acgccgtcgg caatgtcggc
    16081 tcggacgcgc tgtaatcgtc ttggagacag acatcttcgg agatcgccca tgagcgccaa
    16141 aaccgagccc ctggtccccg ccgatcagct cgcccgcgaa agtcccattc gcatccccaa
    16201 cgagagcgtc gaataccggg cggcgcgacg agcgctgctc gcccaggagt tcgagctccg
    16261 ccgccagatc gagcgggtgg cggcaatgcg ccgggcgcta ccgcccggag gtccggttac
    16321 cagtgactat cgattcgaga ccgaggacgg cccaagtgac ctcgcaggct tgttcggcga
    16381 gaagcagacg ctggtggtct acaactacat gttcggtccc gagaggaagc ggccgtgccc
    16441 catgtgcacg aacctgctcg acgcctggga cggcaacgcc gacgacatcg ctcagagggt
    16501 gtcgctcgtg gtcgtcgcgc gctcgccgtt ggagcggctc gcggccgtga agcgcgagcg
    16561 cggctggaaa aacctcaggc tggcgagcgc tctcaccgac gacttcacgc gagcctatgc
    16621 ggggagcccg gacggtggac tggatagtgg agggcccgcc gtcttcaccc gtcgggacgg
    16681 gatgatccgg cacttctggt cgcaggagat gatcgcaagc gaccctggcc aggacccgcg
    16741 cggcgcgccc gacctagccc cattgtggac cgtgctcgac atgacgcccg agggacgggg
    16801 cgcggactgg tatccctcgc ttgagtaccc ggcctagtcg ctcacttcgc ccgccggtac
    16861 cgatcttgag ctcccgcctc gccatcaagc cgcgccgctt gggcacgagc cctgcgcggc
    16921 cgggcgcggc ggcaccgggc gcgcgtgaca gacaaggtgc cgcctcttag gttgggagcg
    16981 aacgcaccgt tcgccggaga gaacccgtgt ctcagaagtg ggaaccgctg ccagccgcag
    17041 gtgtcgacga cgggacgatc ctgttcgacg gcgtctgcgt gctctgttcc gggtgggtcc
    17101 aattcgtcat cgagcgggat ccgaccgggc tgtttcggtt tgtgccgatc cagagcccct
    17161 atggtcgcat gctcgcccag aagttcggca tagacgccga ggcccccgag accaacgtcg
    17221 tagtcgtgga cggaatggcc tacttcaagg ccgacgccgc cttagcggtc ctgacgaagc
    17281 tgccgggatg gtcatgggcg ggaatcagtc ggatcacccc gcggtcggtc aggaactggc
    17341 tttacgacag ggtcgcccgg aaccgttaca ccctcttcgg ccgcaccgac acatgcttga
    17401 tgccgacacc ggacctggcc cgccgcttcc ggaacgagag acccagctga tccatggcgc
    17461 cgaaggtcct tgtcatcgga ggcgccggtg cgttcggtcg ccgtctggtt cgcggcctgc
    17521 tggcgacgac tgatctggaa gtcgttgtcg cgggccgcga tcttgcgcgg gcgcagtcgg
    17581 tggtggacga ggaatgcgtc ggcagtcggg tcggccgagc gaccgccgtc gccctcgaca
    17641 ccaggatcgc gacggccgcc cagcttcgcg acaccggcgc cttcgtcgtg gtcgacgcag
    17701 caggtccatt ccagcgtcag ggctaccact tggctgaaag ggccatcgag gcgggcctgc
    17761 actacgtcga ccttgcggac tcccgcgaat tcgtctctgg ctttcacgtg ctcgatgagc
    17821 gagcccagaa agcgagtgtc accgcgctga ccggggcgag ctcgacaccg gcgctgtcga
    17881 acgcggtcct cgaccatcta acggcgggct ggcaggccgt cgacgacgtc gaaattgcga
    17941 tctcacccgg caaccgggcg ccgcgcggtc tggctgtggt caggtccatc ctctcgtatg
    18001 cgggcaaacc ggtgcgcgtc tgggagaacg gctcgtggcg ccatcgcccc ggatggggcg
    18061 caccggtccg caaggacatc cgcggactgg gcccgcgctg gctgtcgctg tgcgagacgc
    18121 cggacctgga cgtcgtgccc acacggtttg cggtccgccg gtcggtcacg ttcaacgctg
    18181 ggctcgaatt gccggtgatg cacctcggcc tcttcgccgc gacgattgcc atacggctgg
    18241 gactgctgca atccctggag ccgctggcgg agacgttccg ccgggcggcc gacctcctgg
    18301 atggctgggg gacggaccgc ggtgggatgg tcgtcgccgt gtcgggcgtc ggtgctgacg
    18361 gcacgcaggt ctccgccacc tggacactgc tggcggaagc cggtgacggc ccgtccatcc
    18421 cgaccctgcc agcgctcgcc gccgttcgag ccatggcgtc gggcgaactg caccggccag
    18481 gcgcatcggt ctgcgcgggc gtgctccctc tggacgcgat cgagcgagag ttcgccccct
    18541 atcggataac gacgcacgtc gaattgggga cacggcgcga atgacctcgt ccgcgtcgcc
    18601 gttccaacgc gtcctggggc agagcctcga ggacctggcc gggcctgttc ggcgcctgca
    18661 cactttgacg gcgccgacaa gagtatcggg tctcgccgac atcgacgtcg cacctggcgc
    18721 gatccaggcc gcgatatgct ggctggctgg cttgcccaaa cccgggcgcg atgttcccgt
    18781 ttccgtcgag ttccgaccgg acggccgcgg aggcgagcat tgggcccgcc ggttctccgg
    18841 acgcggctac gccagttcgt tcagcgcgga acaacgaggt ggcgaatccc tcctcgtcga
    18901 acgcttcggg cccttccgcc tcgaattccg gctccgagcg gtctcgggtg ggatctgctg
    18961 gctcctcgtc ggcgtaacct gtgtcgggat ccgcgcgccc gtttgggcga tcccgaaggt
    19021 gcgatgcctc gagagcagcg aagacgctcg ataccggttc gacatcgaag cggacatacc
    19081 gctgatcggc ccgctcatca ggtacagggg ctggttggcg tagtccggga gcggaaccca
    19141 tgcgccgccg ggaagcggac cgcgagcgtg tagagagctc gatggtcgca gaccgctgtc
    19201 gcgagctgaa tggtgcttgc ccggcgccga aagggacggg atgcttccgg gcccgaacga
    19261 agtcagtccc gcccgacgcg ccctggaggc cattgatatg aacacgctgc cgtcccggct
    19321 cgaggattcg accgttccgc gtccggcctt cgcggactgg ctcgggacga cgaacgacgt
    19381 caccaagcgc ttcctctcgg cgggacgtgt gcccggcctg atcaacatgg ggggcggcct
    19441 tccggccccg gagacctatc ctgctgccga gttggcggcg atagcccagc gggcgatctt
    19501 ggagcatccg caggaatgcc tgggatacgg accgatcgac ggactgatcg aactgcgcga
    19561 cgtgctcgcg gaacggttca gcacggaaag cctgcggctc gggcgcgaga acgtgttgat
    19621 cacgacgagc ggaatgcagg ggctcgacct cctcggcaag gtcctcctgg aaccgggtgg
    19681 cctgatcgcc ggacagtacc cgacatacct tggcgccctg gacgcctgga aatcgcgaag
    19741 cccgacctat cgcaacatcg tcttggagga cccggacttc gagccggtcg ccgtgttggc
    19801 cggagcgcag ttcgcttaca cggtccccaa cttttcgaac cccaccggca agctggttgg
    19861 gttggagatt cgacaggccc tcgtcgacgc cgcgcacgcc acgggcacct ggctggtcga
    19921 agacgatccc tacggcgggc tgcactacga cggcgcgccc ttgccgcgga tgatcgagct
    19981 gtcgagccgg aggcggcgcg gccagatcta cgacgggccg attgtctaca tgggcacgct
    20041 ctcgaaggag atcgcgcccg gcctgcggat cggttgggtg gtcgccgcac ccgagatgat
    20101 cgacgcgctg accacggcga agcagggatc ggacatgtgc acgagcggcg tcacgcagcg
    20161 gatcgccctc ggcgcgctcg agagcggtct gatcgagcgc ctgcagccgg tcatcgtggc
    20221 cctctaccgc gatcgtcgcg acgcgctctg cgccgctttg gctgaacacc tggccaactc
    20281 gttcacgtgg gaagtccccg ttggcgggat gttcgtgtgg gccgtggcgc gggatccctc
    20341 ggtcgacacc gaccggctgt ggtcggtggc gttggaggcc ggggtgtgcg tcgcgccgag
    20401 cagcgtgttc gacgcgtcgg gacgcaaccg gcgggcgcta cgcctgaact tcacgctcaa
    20461 tccgcccgac aagctggccg agggcgcgcg ccgcctcgcc gccgccgtcc ggacaatgtc
    20521 gaagcagggc tgacaacgcc gccgccgaat gtaccttccc ctatcatccg tgccggtttg
    20581 gaccatccag ttccacaagg attggccagt ggtctgaccg gccgcgatcg aggattgcgg
    20641 atccagtgct catcagaccg cgggcgaggc accggtccag gcggaagggc aagatcccct
    20701 tggcgaagtg cgtcgtccct cgcggcccca cgtcggggaa gctcggcagc cgcaccggac
    20761 cgactacgtt catgtcgcca atgataacag cagcgcccgt caggtgggcg gcgacatgcc
    20821 gaagctggcg ccgcaatagg gcttgcccgt gcgccaaatg gacgttcacg atcgtgacgc
    20881 cagccaatcg caaaatggcg gcgaaccgcc gatcacgcgt ccggctgttc ggattgaacg
    20941 gcaggtccag gacgtgcgcg gggctccaga tcccaagacc gtggatcctg tggagcatct
    21001 atcgccgcca aaaatggccc ttggcgagcg tcgctaagga aacattgccc cgagccccac
    21061 agacccgcta tcgatgatgg cgtttcgcca ggcggatagt gtgattatgg aaaccacgat
    21121 cacggtccga aacctgcgcc ccttgaatgg gcgagtctat gcgctcgtcg acgccgacat
    21181 cgtgatcggc gatatcgaga tcaccgtgtt cggcatccag gcccggcacg tgccctcggg
    21241 cgggacctcc atccatctgc cgacgtaccg tgatggggac ggcgcatggc gagccgcggt
    21301 ggagcttccc gaggaaattc gtgaggcgct ctgcgatgcg atcttggcct tcatggtcga
    21361 ggagggcgtc gccaagccgc gacaagcggc ggccgaccgt gaagatgcta ccgcgactgc
    21421 cgctgagata ccgtcagggc aggatgattt cggcaagcgg ctggtggcgc gaaagggctc
    21481 gctcaccgcg gatgtcgatc tagggattta gcgatccgac ttcacgccaa gccaacggtc
    21541 agattaggcc tatcccgcct tctggaaagc cgtgacacaa cctaaccgcg cagtctctcg
    21601 gcgcgccggg aacacgccgt cccgatcctc gacgatctca agcaatgggt cgaagcgcag
    21661 ctacggcgcc tgtcgtcgaa gagcacgctc agcaaggcga tccgatatgc gctgacacgc
    21721 tgggattcgc tcaccctcta tgtcgccgac gggcgcatcg gcatcgacaa caatccagcc
    21781 gaacggtccc tgcgtgggat tgcgctcacg agaaagaatt tcctgttcct gggatcagag
    21841 gcgggcggcg atcgtgccgc gatcctctac acggtgctgg agacggcgtt atgccgcgcg
    21901 cggcataagg ccgtttatgc cgaaggccga actatgccga gcggcgcagt tattcatagc
    21961 attatcaacg atcttcgtcc gctcggttcg gcataaatag tcctctgtct ccgccttcaa
    22021 cagcggagaa gagaatggtg acgacagagg
//
LOCUS       DATGEM010000641         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1643471, whole genome shotgun sequence.
ACCESSION   DATGEM010000641 DATGEM010000000
VERSION     DATGEM010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643471"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VKQ27_19810"
     CDS             <1..824
                     /locus_tag="VKQ27_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91240.1"
                     /translation="ECITLPHSAEARYAVEVGRVAFRTPMLLGGQAARAGLACESCHR
                     NGRTNPVFSFPGLSGAPGTADVTSALFSTRRDDGIDNPRPIPDLGGDKHRLRIDQSTA
                     SGALPAFIHGLVVDEFDGHEPPAAVLAGLAAYVRAMDPASCAHKDAVPVTAEGLITEA
                     GRAVRAAVMALDHNDPATAILMVEGARWQLGLIDERYALPGLEPQRALIRSAALDLTA
                     ALAAIRIDGHSARDQLTAWQARMPAWEKAIKDREAASLFEPSVIMAHLATNTGAP"
     gene            complement(932..3928)
                     /locus_tag="VKQ27_19815"
     CDS             complement(932..3928)
                     /locus_tag="VKQ27_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY91241.1"
                     /translation="MTSRTRISVLSLAIALAGSACAVPACAQAAGGTLARQTMFNIPS
                     GDLGGAISAFSAATGLQVVAPPAEVSGRRTQGISGSHRAGEALDALLRGTGLRASLSS
                     GVVVLKATSPFHRISQTIVAPEEAPDGAERAVADTTESPQIIVEGYRESLRLAQDYKR
                     KAVGAEDDILASDIASFPDLNLAESLQRVPGVTINRDSGEGRQVTLRGLGPDFTRTQL
                     NGMEVLGNTASGMDNRGAVSRTRSFDFSLFASELFNRVSVQKSYAADQDEGGIAGTVQ
                     LYTARPFDYSGAKFVVSAKGQINTNTSGVTPRVVALASDRWGDFGALVSVAYSEIKNN
                     EYGYRNWGWGKVTYNAANIGPNIDPQTAALLRSGTLYAPQAESPSTWYTDRKRLGVTS
                     ALQYHGDTLKLDLDLLYGRMWDHRDDWAIAAAGSNALTGSPIGGTQVIQSATIVGNTL
                     EAATYTGIDQRSEHHVVENHTDFYQGAFNGSWQATDRLKFTALGGYEQSNFRQPVFDK
                     VFLESKNHSFSFDNQPTIPVNTYGFDLTNPANWQLMRLDTQENAITSKYSNGKLGASY
                     QLSDASNFEVGGEYKHFTNDGYIYVHKVFHNVGGDTVIPDNLKDVINRDSLIPYIGGN
                     IDGIYSLIGDPRDLNASFLQAGSDFRVAEKTWSAYAQYNLDTWLMGMEVRANAGVRYY
                     DTALNSSGHLAAPVNGVTTLVPVSITTHSHGWLPAANLAVGIRKDLIARLSVSRNVNR
                     PALSDLAAAGSITTRPNGGSVSFGNPYLKPYEATSVEGSLEYYMSRQGFASVGFFYKS
                     MDSFISPTTMQLPYSRTGLPLSLLIQGEDGNTLFDVSRPVNGDGANIKGVELSFQHDF
                     TFLPGALKHLGINANGTWFDGHQSTIINGAAVRIPLFNLSKWAANATLYYETDRWGAR
                     ISDAYRSRYLTSGGSGGNVGDGIRGTNNVDFQAHYNINPRLRVVVEGINITNEAIQQF
                     TDLTADRTEVYTTSGRTFTFGVTAEF"
     gene            complement(4053..4910)
                     /locus_tag="VKQ27_19820"
     CDS             complement(4053..4910)
                     /locus_tag="VKQ27_19820"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HLY91242.1"
                     /translation="MMRGIADTDLRDDLRSDSALREALQASAAAGRLSNDEIRAMRAA
                     RRRRTVSVAAIALVAVVGFGGWRQWMIPTPTAPAVALHFGTKPGETRVVELADGSSIQ
                     LGGGSRVDVNLGPDRREVRLEAGEAYFDVAHDPSKSFTVRAGATDVRVLGTAFNLNLT
                     QEQVGLAVYRGAVRFAGASAGKQVVTAGYRTRFHQGRIDSPVHFDPMQPDWRLGWLDT
                     AGMTLGDLVEVLDRQGGPSIAPPPPALAGIAVSGRFRLDNSGQLLDAIGSANGFHVRR
                     EAHRLVIGP"
BASE COUNT          865 a         1605 c         1649 g          877 t
ORIGIN      
        1 ccgaatgcat cacgctgccc cattcggcgg aggcacgcta tgccgtggag gtcggccgcg
       61 tcgcattccg gacaccgatg ctgctgggcg gtcaggcggc gcgtgccgga ctggcctgcg
      121 aaagctgcca ccgcaacggc cgcaccaatc cggtcttttc cttccctggc ctgtcgggcg
      181 cgccgggtac ggcggacgtg acgtccgccc tgttcagcac gcgccgggac gatgggatcg
      241 acaatccccg cccgatcccc gatcttggcg gcgacaagca tcggctacgt atcgaccaga
      301 gcaccgccag cggtgcgctg cctgccttca tccacggcct cgtggtcgat gaattcgacg
      361 gtcatgagcc gccggccgcc gtgctcgccg gtctggccgc ctatgtgcga gcgatggatc
      421 cggcaagctg cgcgcacaag gatgccgtcc ccgtcacagc cgagggtctg atcacggagg
      481 caggtcgtgc cgttcgtgcc gccgtgatgg cgctggatca caacgatccg gcgacggcga
      541 tcctcatggt ggaaggcgcc cgctggcagc tcgggctgat cgacgagcgc tacgccctgc
      601 cgggccttga gccccagcga gcgttgatcc ggtctgcagc ccttgacctg acggccgcgc
      661 tcgccgcgat ccgcatcgac ggccattctg cccgcgatca gctcacggcc tggcaggccc
      721 gcatgccagc gtgggagaag gccatcaagg accgcgaagc ggcctccctc ttcgagccga
      781 gcgtgatcat ggcgcacttg gccaccaaca ccggagcacc ttgatctgta gcaggaggga
      841 cgacgcccaa accgcgccaa aagagcacgt ccacttccga aaccctaagg cccgaaagcg
      901 gacgcgacat ctctcgattg cccggcctgg cctagaattc ggcagtgacg ccgaaggtga
      961 aggtacggcc gctcgtcgta tagacctccg tgcggtcggc ggtcaggtcg gtgaactgct
     1021 ggatcgcctc gttggtgatg ttgatgcctt ccaccacgac gcgcaggcgc gggttgatgt
     1081 tgtagtgcgc ctggaagtcg acgttgttgg tcccacggat accgtcgccg acattacccc
     1141 cgctgccacc actggtcaga tagcggctcc ggtaggcgtc cgagatacgc gcgccccagc
     1201 gatcggtctc gtaatagagc gtcgcgttgg ctgcccactt ggagaggttg aacagcggga
     1261 tccggaccgc cgcgccattg atgatcgtgg actggtggcc gtcgaaccag gtaccgttgg
     1321 cgttgatgcc cagatgcttc agcgcacccg gcaggaaggt gaagtcatgc tggaaggaca
     1381 gctcgacacc cttgatgttg gcgccatcgc cattgaccgg acgactgacg tcgaacagcg
     1441 tgttgccgtc ctcgccctgg atcagcaggg atagaggcag gccggtccgg ctgtagggaa
     1501 gctgcatcgt cgtcggcgag ataaagctgt ccatgctctt gtagaagaag cccaccgagg
     1561 cgaatccctg ccggctcata taatattcga gcgagccttc caccgaggtc gcctcgtagg
     1621 gcttcagata ggggttgccg aagctcacgc tgccgccgtt gggccgcgtc gtgatcgagc
     1681 ccgccgccgc gagatcggac agcgccggcc ggttgacgtt gcggctgacg ctgaggcgcg
     1741 cgatcagatc cttgcggatg ccgaccgcga gattggcggc gggcagccag ccatggctgt
     1801 gcgtcgtgat tgacaccggc accagcgttg tgacgccgtt gacaggagcg gccagatggc
     1861 cggatgagtt gagcgcagtg tcataatagc gcacgcccgc gttggcgcgg acctccatgc
     1921 ccatcagcca ggtgtcgaga ttatactggg cgtatgcgct ccacgtcttc tcagcgacgc
     1981 ggaagtcgct gcccgcctgg aggaaggaag cgttgagatc gcgcggatcg ccgatcagcg
     2041 aatagatgcc gtcgatgttg ccgccgatat aggggatcag cgagtcccga ttgatcacgt
     2101 ccttcagatt gtcgggaatg acggtgtcgc cgccgacgtt gtggaacacc ttgtggacgt
     2161 agatgtagcc gtcgttggtg aagtgcttgt actcgccgcc gacctcgaaa ttcgaggcat
     2221 cgctgagctg gtagctcgcc ccgagcttgc cgttggaata tttgctggtg atcgcatttt
     2281 cctgcgtatc gaggcgcatc agctgccagt tggccgggtt ggtgagatcg aagccatagg
     2341 tgttgaccgg aatggtcggc tggttgtcga agctgaagct gtgattcttc gattccagga
     2401 acaccttgtc gaacaccggc tggcggaagt tggactgttc atagccgccc agcgccgtaa
     2461 actttagccg gtcagtcgcc tgccagctgc cgttgaacgc accctgatag aaatcggtat
     2521 ggttctcgac gacatggtgc tcggagcgct gatcgatgcc ggtgtaggtc gccgcctcca
     2581 gcgtgttgcc gacgatggtg gcggactgga tcacctgcgt gccgccgatc ggcgagccgg
     2641 tcagcgcgtt ggaaccggcc gccgcgatcg cccagtcgtc gcgatggtcc cacatccgtc
     2701 cgtagagcag atcgaggtcc agcttcaacg tgtcgccatg atattgcagc gccgaggtga
     2761 cgcccaggcg cttgcgatcg gtgtaccagg tcgacggcga ttccgcctga ggggcgtaga
     2821 gggtgccgga gcggagcaac gccgcggtct gcggatcgat gttcggcccg atattggcgg
     2881 cgttgtaggt gaccttgccc cagccccagt tgcggtagcc atattcgttg ttcttgattt
     2941 cggaataggc gaccgaaacc agcgcgccga aatcgcccca tcgatccgat gcgagcgcca
     3001 cgacgcgcgg ggtgacgccg ctggtgttcg tgttgatctg tcccttggcg gagacgacga
     3061 acttcgcccc gctatagtcg aaaggcctgg cggtatagag ttgtaccgtg ccggcaatgc
     3121 caccctcgtc ctgatcggcg gcatagctct tctgcaccga gacgcggttg aacagctcgg
     3181 aagcaaacag gctgaagtcg aacgagcggg tacggctgac ggcaccgcga ttgtccatgc
     3241 ccgaggcggt gttgccgagc acttccatgc cgttgagctg cgtgcgcgtg aaatccggcc
     3301 cgagaccgcg cagcgtcacc tgacggcctt cgccgctgtc gcgattgatg gtgacgccgg
     3361 gcacgcgctg gagcgattcc gcgaggttga gatccgggaa ggaagcgatg tcggaagcga
     3421 ggatgtcgtc ctccgcgccg actgccttgc gcttatagtc ctgcgcgaga cgcaggctct
     3481 cgcgatagcc ttcaacgatg atctgcggcg actccgttgt gtcggccacc gcgcgttcgg
     3541 caccatccgg cgcttcctcc ggcgccacga tcgtctggga gatgcgatgg aacggagacg
     3601 tcgccttcag cacgaccacg ccgctcgaca gtgaggcccg aagccccgtc ccgcgcagca
     3661 gcgcgtcgag cgcctcgccg gcgcgatggc tgccgctgat accctgcgtc cgccgcccgg
     3721 agacctcagc gggtggtgcc acgacctgaa gcccggtcgc cgccgagaag gctgaaatgg
     3781 cgccgccgag atcacccgag ggaatgttga acattgtctg ccgggccagc gtgccgcccg
     3841 ccgcctgcgc gcaggcgggc acagcgcagg cactgcccgc caaggctatg gctaggctca
     3901 ataccgaaat acgggtccgg ctcgtcatgt cttgtcccct ttgtgcggcc cgtttcggag
     3961 ggcttcgatc agggagacgg cggcggccgc ccggtcagga caaaataatt ttgttaaata
     4021 attcagtcgg taatacgacg attttgttgc gattaaggcc caatgacgag gcggtgcgcc
     4081 tcgcgccgga catggaagcc gttggcgctg ccgatggcat ccaaaagctg ccccgaattg
     4141 tcgaggcgga agcggccgga aaccgcgatt ccggcgagcg cgggcggcgg cggcgcgatg
     4201 gagggcccgc cctgccgatc gagcacctcg accagatcgc caagcgtcat cccggccgtg
     4261 tccagccagc ccagccgcca gtcgggctgc atcggatcga aatggaccgg gctgtcgatg
     4321 cggccctgat ggaagcgagt gcgatagccc gccgtcacca cctgcttgcc ggcggacgcc
     4381 ccggcgaagc gcaccgcgcc acgatagacc gcaaggccga cctgttcctg cgtcagattg
     4441 aggttgaagg cggtgccaag cacgcgcacg tcggtcgccc cagcgcggac ggtgaacgac
     4501 tttgatggat cgtgcgccac gtcgaaatag gcctcgcccg cctccagccg cacctcacgc
     4561 cggtccggcc ccagattcac gtcgacgcga ctaccgccgc cgagctggat cgaggaacca
     4621 tccgccaact cgacaacgcg cgtctcgccc ggcttcgtcc cgaaatgcag cgcgacggcc
     4681 ggggcggtcg gggtcggaat catccattgc cgccatccgc cgaagcccac cacggcgacc
     4741 agcgcgatcg cggcaaccga caccgtccgg cggcggcgcg cagcgcgcat cgcccggatc
     4801 tcgtcgttgg acaggcggcc ggcggctgcg ctcgcctgca acgcttcgcg cagcgccgaa
     4861 tcagatctga gatcatcccg caggtcggta tcggcgatgc cgcgcatcat gcgtcccccg
     4921 agaagttgag cccacgccgt gcgagcgccg cgcggagatt ggacagcgcg cgtgtcacat
     4981 gcttttcgac tgccgc
//
LOCUS       DATGEM010000642         3742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1298917, whole genome shotgun sequence.
ACCESSION   DATGEM010000642 DATGEM010000000
VERSION     DATGEM010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298917"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="VKQ27_19825"
     CDS             complement(<1..2057)
                     /locus_tag="VKQ27_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HLY91243.1"
                     /translation="METGAATTPPLHAVLIDGSGFIFRAYHALPPMTRPDGTPVNAVF
                     GFSNMLAKLLREHVGTHIAVIFDAGAQTFRNRLYDAYKAHRPPPPDDLIPQFKLVREA
                     TAAFGVPAVELEDWEADDLIAAYAKAVTDAGGQATIVSSDKDLMQLIRPGVEMLDPIK
                     QKPIGPAEVMEKFGVTPDKMIEVQALIGDSVDNVPGIPGIGPKGAAQLINEYGDLESV
                     LAAAPGMKASKRRDNLLEHAEKARISRELVTLRTDTPMPLPIDQMHARPFDKAALGAW
                     LKTQGFRSIATRLGVDGEVAAPVSTTVEVAPRRAQAALPLPEAERPAIAEAASGFGPY
                     ITITNESALRDFLMETAACGFLAIDTETDGLNPLQAPLVGLSLAVAAGRAAYLPLRHA
                     GLAEQVPLAAAIAALRPILTDPNTLKIYQNAKFDMLAMERAGFPPAAPFDDVMLISYA
                     QEAGMHGHGLDELSQLHLGHTPTTYDEVTGTGRNRIAFSRVPIDRATAYAAEDADVAL
                     RLWQALRPRLRFNGALTLYEQVERRLLPVLIDMERAGIKVDADDLRRMSVDFERRMGV
                     IEQDIHKLVGHAFNVGSAKQLGEVLFDELKLGGGKRMKTGAWGTDASVLQSLADQGHE
                     LPARIMDWRQLQKLKSTYADALVGEINPDTGRVHTSYAQCIASTGRLSSNDPNLQNIP
                     IRT"
     gene            complement(2281..2475)
                     /locus_tag="VKQ27_19830"
     CDS             complement(2281..2475)
                     /locus_tag="VKQ27_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HLY91244.1"
                     /translation="MDATTGSAPVETIEVDDATVACDGGDGALGHPRVFLHIEGRSVM
                     CPYCSRLYVLKEGAGHAGGH"
     gene            2474..3544
                     /locus_tag="VKQ27_19835"
     CDS             2474..3544
                     /locus_tag="VKQ27_19835"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY91245.1"
                     /translation="MMALRILYGGSGRYDSDATHAFNHGSPPGMRHRLLTALVAAALL
                     LSGCTTHLIPPGPSVAPPRETADAFVMPDGTRLPYRVWLPDGKPWAVVLAFHGMNDSR
                     DAWEYPAPELAAEGVAVFAPDQRGFGATEGRGYWPGTEALVADARDMALLLHQRYPHT
                     KLFLMGESMGAAVLMCLATAPDPPPVAGYVLISPAVWGRAEMNVFLRVGLWLFANLIP
                     GFTATGQLAHVTASDNREAIRRLSTDPLTIHKTRFDTVRGLVNLMDAALAAAPEFGAR
                     ALFMYGGKDELIPKKATVATWKPLPPGPVLAYYPDDYHLMLRDHERQAPIDDILAWMR
                     RPDDPLPSGADRAAQAWLAKQD"
BASE COUNT          613 a         1227 c         1227 g          675 t
ORIGIN      
        1 tcggtgcgga tcgggatgtt ctgcaggttc ggatcgttgg acgacagccg tccggtggac
       61 gcaatgcact gtgcatacga cgtgtgtaca cgaccggtgt cgggattgat ctcgcccacc
      121 agcgcatcgg cgtaggtgga cttcagcttc tgcaactgcc gccagtccat gatgcgggcc
      181 ggcagttcgt gtccctgatc ggccagtgat tgcagcacgg acgcatcggt gccccaggca
      241 ccggtcttca tgcgcttgcc gccgccgagc ttcagctcat cgaacagcac ctcgccgagt
      301 tgctttgcgg agccgacgtt gaaggcatgg ccgacgagct tgtggatgtc ctgctcgatc
      361 acgcccatgc gccgctcgaa atccaccgac atgcggcgca ggtcgtccgc gtccaccttg
      421 ataccggcgc gctccatatc gatcagcacc ggcagcaggc gtcgctccac ttgttcgtac
      481 aacgtgagcg cgccgttgaa ccgcagccga ggccgcagcg cctgccagag ccgtagcgcc
      541 acatccgcat cctccgcggc ataggcggta gcgcgatcga tcggcacccg ggagaacgca
      601 atgcgattgc ggcccgtacc cgtcacctcg tcgtaagtgg tcggcgtgtg gccaaggtga
      661 agctgcgaca gttcgtcgag cccatggccg tgcatgccgg cttcctgggc ataggagatc
      721 agcatcacat cgtcgaaggg tgcggcgggc gggaatccgg cacgttccat cgccagcatg
      781 tcgaatttgg cgttctggta gatcttcagt gtgttcggat cggtgaggat cggtcgcagc
      841 gcggcaatgg ccgctgcgag cggcacctgc tccgccagcc ctgcgtgccg cagcggcaga
      901 taggccgcac gacccgccgc cactgccagc gacagcccga ccagcggcgc ctgcaacggg
      961 ttcaggccgt cggtctcggt gtcgattgcg aggaacccgc aagccgcggt ctccatcagg
     1021 aaatcgcgca gtgctgattc gttggtgatc gtgatgtagg gaccgaaccc ggaagcggcc
     1081 tcggcaatcg caggacgttc ggcttctggc aatggcaaag ccgcctgcgc gcggcgaggc
     1141 gccacttcga cggttgtgga aacgggcgcg gcaacttcac catccacgcc gagccgcgtg
     1201 gcaatggaac gaaagccttg tgtcttcagc caggcaccaa gcgcggcctt gtcgaacggc
     1261 cgagcgtgca tctgatcgat cggcagtggc atcggcgtat cagtgcgcaa cgtcaccaat
     1321 tcgcgggaaa tgcgtgcctt ctcggcatgc tcgagcagat tgtctcgccg cttggaggcc
     1381 ttcatgccgg gtgcggcggc cagaacgctc tccaggtcgc catattcgtt gatgagctgc
     1441 gccgccccct tcggcccgat acccgggatg cctggcacgt tgtccaccga atcgccgatg
     1501 agcgcctgca cctcgatcat cttgtccggg gtgacgccga atttttccat cacctcggcg
     1561 ggaccgatcg gcttctgctt gatcgggtcc agcatctcca cgcccgggcg gatgagctgc
     1621 atcaggtcct tgtcggacga cacgatcgtc gcctgtccgc ccgcgtcggt caccgccttc
     1681 gcataggccg cgatcaggtc gtcggcttcc caatcctcca actcgaccgc tgggacaccg
     1741 aacgccgcgg tcgcctcgcg caccagcttg aactgcggga tcaggtcgtc cggcggcggc
     1801 gggcgatgcg ccttgtaggc gtcgtagagc cggttgcgga aggtctgcgc ccccgcatcg
     1861 aagatcaccg cgatatgggt gccgacgtgc tcgcgcagca gcttcgccag catgttggag
     1921 aagccaaaaa cggcattcac cggcgtgccg tccggccgcg tcatcggcgg cagcgcgtga
     1981 taggcgcgga atatgaagcc ggacccgtcg atcaggaccg cgtgcagggg gggcgtcgta
     2041 gcggcgccgg tttcgatgtc cggcaggatg tctgtatcgg cgggcacgat ggctcctgtg
     2101 cctggcggtg gcgtggcatt tcgccacggt gggctgtgtg tcagccagcc gggatgatag
     2161 ccgggcgcgg caaggtttca aaatgccggg actgttccga cgccgaatgc cactggcttc
     2221 agttggtcgc gtcagcgcgc cgtgtcagcg gagccccacg tcggcccggg aaccagccgg
     2281 tcaatgccct ccggcgtgcc ccgccccctc cttcagcacg tagagccgcg agcagtacgg
     2341 gcacatcacc gaccgtccct cgatgtggag gaacacccgc gggtggccaa gtgcgccatc
     2401 gccgccatcg caggcgaccg tggcgtcatc gacttcgatc gtctcgacag gcgccgaacc
     2461 agtggtggcg tccatgatgg cccttcgcat cctctatggc gggagtggcc gctatgatag
     2521 cgatgcgacc catgctttca accatggttc gccgccgggc atgcgccatc ggctgctgac
     2581 ggcgcttgtc gctgccgctc tgctgctgag cggctgcacg acgcacctga tcccccccgg
     2641 tccgtccgtt gcgccgccgc gcgagacggc ggacgcgttc gtcatgccgg acgggacccg
     2701 gctgccctat cgggtctggc tcccggacgg aaagccgtgg gcggtggtct tggcattcca
     2761 cggcatgaac gacagccgcg acgcctggga atacccggcg cctgaactcg ccgccgaggg
     2821 tgtcgccgtg ttcgcccccg atcagcgagg gttcggcgcc accgaaggcc gcggctactg
     2881 gccgggcacc gaggcattgg tcgccgatgc gcgcgacatg gcactcctac ttcaccagcg
     2941 gtacccgcat accaagctgt tcctcatggg cgagagcatg ggggctgcgg tgttgatgtg
     3001 cctggccacc gcgcccgatc ccccgccagt cgccggctat gtgctgatct cgccggcggt
     3061 ctgggggcgg gcagagatga acgtgttcct ccgtgtcggg ctctggttgt tcgccaattt
     3121 gatccctggc ttcaccgcca caggccagct cgcgcacgtc accgccagcg acaatcgcga
     3181 ggcgatcaga cggttgtcga cagatccgct caccatccac aagacgcgct tcgacactgt
     3241 gcgcggcctg gtgaacctga tggatgcggc gctcgccgca gcccctgaat tcggtgcccg
     3301 cgccctgttc atgtacggcg ggaaggacga actgatcccc aagaaggcca ctgtcgcgac
     3361 ctggaagcct ctgccgccgg gaccggtgct cgcctactac cccgacgact accacctgat
     3421 gttgcgcgat cacgagcggc aggcgccgat cgacgatatc ctggcctgga tgcgccggcc
     3481 ggatgatccg ctgccgtcgg gagcggatcg ggcggcgcaa gcatggctgg cgaagcagga
     3541 ctgacgccgg gctatgatcc agcgccgttt cgcgctatgg ggttcggcgt tggacccgta
     3601 gctcagttgg atagagcgtc gccctccgaa ggctgatgtc aagggtgttt ggtttaggag
     3661 tgagtatcga aaggccttaa aaagcagtac cttgaacgca cctcaacacg atggatcggc
     3721 acctgagttt ttcgcgtaag ca
//
LOCUS       DATGEM010000643         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_959182, whole genome shotgun sequence.
ACCESSION   DATGEM010000643 DATGEM010000000
VERSION     DATGEM010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959182"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VKQ27_19840"
     CDS             <1..328
                     /locus_tag="VKQ27_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_except=(pos:77..79,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HLY91246.1"
                     /translation="YSAPADADIVDELRRWQAVVTGVGDUGSCSSCSLHDSITAERLG
                     IPAMAVMTTRFVSAAELMARVLGFPGYAFATIDHPISSAGDDELRAMAAVAVDQMRRS
                     LLHNTS"
     gene            344..673
                     /locus_tag="VKQ27_19845"
     CDS             344..673
                     /locus_tag="VKQ27_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLY91247.1"
                     /translation="MATKPGSARDVVRGTVPKMIEVAEQVIYGDVWERPGLSKRDRSL
                     IVVASLIATYRPEQLRGHLARALDNGVTKDEIAEVITHLAFYSGWPASMTAANIAREV
                     FEERGLT"
     gene            complement(670..1551)
                     /locus_tag="VKQ27_19850"
     CDS             complement(670..1551)
                     /locus_tag="VKQ27_19850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLY91248.1"
                     /translation="MIQPPTHQIPGVYHRRIGDIVVTALSDGYLEGGVDVLQNITPDD
                     ALHALNENFWPGRRTSVNCFAIYSAGRLALIETGSGNYLQPTAGKLQKNLAAAGIDPA
                     DVEKVILTHMHPDHSAGLTDPKTGTKNFPNAELIVHENEPRHWSDDAAMAKAAERSKR
                     LYFQCAREQMAPYHNVMHTFSAETEVFPGVRSIPITGHTPGHTGYMIVSGNESLFIWG
                     DIVHAPDIQIPRPEVTIEHDNDPHAAAATRKRVLDWVTTDRQLVAGMHMHFPGFAHVV
                     RRNGSYMMLPESWDQTF"
     gene            complement(1683..2630)
                     /locus_tag="VKQ27_19855"
     CDS             complement(1683..2630)
                     /locus_tag="VKQ27_19855"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLY91249.1"
                     /translation="MTELLQPDAEGIARAAQLLRDGKLVSFGTETVYGLGGDATNDRA
                     VAAIFAAKERPSFNPLICHYPNADAAYRTVVANERAHRLAEAFWPGPLTLVLRRTVDC
                     PVSLLAGAGLDTLAVRVPAHDIARALLRQIDRPVAAPSANRSGRISPTTAQHVLAELS
                     GRIAAVLDCGPCSVGVESTVLDLSGKDAVLLRPGGVTLEAIEATIGAVAHHDTPGVLR
                     SPGLLASHYAPRTAVLLNVSEVRPDEALLAFGAALPGAGSTFNLSEASDLTEAAARLF
                     AGLRALDAAGQRRIAVMPIPERGLGRAINDRLRRAAAPR"
     gene            2735..4222
                     /locus_tag="VKQ27_19860"
     CDS             2735..4222
                     /locus_tag="VKQ27_19860"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HLY91250.1"
                     /translation="MLDVAFAKPALPKSGAVVLLIGEGEMPSGLWQQADEATGGAIAR
                     ALAAAEFKGAKGKTCMILAPGAGLSRVLAVGLGKPAELKPLGLNEAGGAIAAAMPREA
                     AVAIATGTLQPALAAEVAFGAVLRAYRFDKYRTKEKAEDKPKLGKITVLTAEPPRAKA
                     AWEPLEGVANGIFLTRDLVSEPPNVLNPAEMADRCKKLSELGLKVEILGPREMTRLGF
                     GALLGVAQGSVNEPRMVVMQWNGASGSGRKRGSSGKPVAFIGKGVTFDTGGISIKPAG
                     GMEDMKWDMAGAGTVVGLMAALAGRKAKVDAVGLVGLVENMPSGSAQRPGDVVKTYSG
                     QTVEVINTDAEGRLVLADVLWYCQQKFDPRFMVDLATLTGAMIVALGHEYAGMFSNDD
                     ALAQKLSAVGSDVNEKVWRFPLHDEYDKLIRSNIADMKNIGGRPAGSISAAQFIQRFV
                     NNKPWVHLDIAGMAWSGKDTACVPKGATAFGVRLLDRLVAQHFEG"
     gene            4317..4763
                     /locus_tag="VKQ27_19865"
     CDS             4317..4763
                     /locus_tag="VKQ27_19865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HLY91251.1"
                     /translation="MTDIGFYHLTRTGPDKALPQLLGRTLAAGQRAVVLCADVERLAA
                     LDAALWQCAEPDWLPHGTAADGDADLQPVWLTTEVAAPNGARFLFLLDGLRSERLGEF
                     DRVFDLFDGQDEAAVQAARERWRAAKGAGHELTYWQQGAKGWEKKA"
     gene            complement(4738..>4933)
                     /locus_tag="VKQ27_19870"
     CDS             complement(4738..>4933)
                     /locus_tag="VKQ27_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91252.1"
                     /translation="RGAEGSLPRDAMVRWLEAVGLDQSVRRRGIAVMPPSQAGLLAGT
                     PGIRVLKPLGERLTPSSPNP"
BASE COUNT          861 a         1437 c         1776 g          859 t
ORIGIN      
        1 ttacagcgct ccggccgacg cggacatcgt cgatgaactc cgtcgctggc aggccgtcgt
       61 caccggggtg ggcgactgag gcagctgctc gtcgtgcagt ctgcatgact ccatcaccgc
      121 tgaacggctt ggcatccccg cgatggcggt gatgaccacg cgtttcgtca gcgcggccga
      181 gttgatggcg cgcgtgctcg gctttcccgg ctacgcgttc gcgacgattg accacccgat
      241 cagcagcgcc ggcgacgacg agctgcgcgc gatggcggcg gtggcggtcg atcagatgcg
      301 gcgatcgctg ctacataaca cgtcgtaaca agaggagacg accatggcca ccaagcccgg
      361 cagcgcgcgc gacgtggttc gcggcacggt tcccaagatg atcgaggtgg ccgagcaggt
      421 catctatggc gacgtgtggg aacggcccgg gctgagcaaa cgcgaccgga gcctgatcgt
      481 ggtcgcgtcg ttgatcgcga cctatcgccc ggagcagttg cgcggccacc tcgcgcgcgc
      541 cctggacaat ggggtcacca aggacgagat cgccgaggtc atcacgcatc tggcgttcta
      601 ttccggctgg ccggcctcga tgaccgccgc caatatcgcg cgcgaggtgt ttgaagagag
      661 ggggctgacc tagaaggtct ggtcccagga ttcgggcagc atcatgtagc taccgttgcg
      721 cctcaccacg tgggcgaagc cggggaagtg catgtgcatc ccagcgacca gctgccgatc
      781 tgtcgtcacc cagtccagca cccgcttacg tgtcgcagcc gcagcgtgcg gatcgttgtc
      841 gtgctcgatc gtcacttcgg ggcgagggat ctggatgtcg ggcgcgtgca cgatgtcgcc
      901 ccagatgaaa agcgactcgt tgccggatac gatcatgtag ccggtgtgtc ccggcgtgtg
      961 tcccgttatc ggtatcgagc gcacgccggg gaagacctcg gtttcggcgg agaaggtgtg
     1021 catcacgttg tggtacggcg ccatctgctc gcgcgcgcac tggaaataca ggcgcttgga
     1081 acgctcggct gcctttgcca tcgctgcgtc atcgctccag tgacgtggct cgttttcgtg
     1141 cacgatgagt tcggcgttcg ggaaattttt ggtgccggtc ttgggatccg tcaggcccgc
     1201 ggagtggtcc ggatgcatgt gcgtcaggat cactttctcg acgtcggccg gatcgatgcc
     1261 tgcggcggcg aggttctttt gcagcttgcc ggcggtgggt tgcaggtaat tgccggagcc
     1321 ggtctcgatc agcgcgaggc ggcccgccga gtagatcgcg aagcagttca ccgaggtgcg
     1381 tcggcctggc cagaagtttt cgttcagcgc gtggagcgcg tcatccgggg tgatgttctg
     1441 caagacatcg acgccgcctt cgagataacc gtcggacagc gcagtcacca cgatgtcgcc
     1501 gatgcgacgg tgatagacgc ctgggatctg atgagtcggt ggttggatca tttcggccgc
     1561 ctgtgtcatt gcggacggaa cgaagcaagc tcgggggagt tgcgcaagcg gggacgagat
     1621 tgcctgcccc ggccatcgcc ggggtcgcaa tgacgcgatg cgtcagcgcg gcgacacggg
     1681 cttcagcgtg gggcggcggc gcggcgcagg cgatcgttga tcgcgcggcc aagtccgcgt
     1741 tcgggtatcg gcatcacggc gatacgccgc tgtcccgctg catccagtgc gcgaaggccg
     1801 gcgaacaggc gcgcggcggc ttcggtcagg tcgctcgctt cgctcaaatt gaaggttgat
     1861 ccagcccccg gcaacgcggc gccaaaggcc aacagtgctt catcaggacg gacctcgctg
     1921 acgttcagga gcaccgcggt gcgcggcgcg tagtgcgatg caagcagacc gggcgagcgc
     1981 aagacgccag gcgtgtcgtg atgcgcaacc gcgccgatcg ttgcctcgat ggcctccagc
     2041 gtgacaccgc cgggccgcag cagcacagca tccttgcccg acagatcgag cacagttgat
     2101 tcaacgccaa cactgcacgg gccgcaatcg agcacggctg cgatgcgtcc ggacaattcc
     2161 gccagcacat gctgcgccgt cgtcgggctg atcctgccgg agcggttcgc cgagggagcc
     2221 gcgacaggcc gatcgatctg gcgcagcaac gctcgtgcga tgtcgtgcgc ggggacacga
     2281 accgccaacg tatcgagacc cgcaccggcc agcaagctga ccgggcaatc gactgtgcgc
     2341 cgcaagacca gcgtcagtgg gcccggccag aatgcctcgg ccaaccggtg cgcgcgttca
     2401 ttggcgacga cagtacgata tgccgcgtcc gcgttgggat aatggcagat cagcggattg
     2461 aagcttggcc gttccttggc ggcgaagatg gctgccacgg cccggtcgtt cgttgcgtcg
     2521 ccgcccagcc cgtagaccgt ttctgttccg aacgagacga gcttgccatc gcgcagcagt
     2581 tgggcagcgc gggcaatgcc ttcggcatct ggctgtaata actctgtcat atagcgcaca
     2641 taacgatggg tcgtgggcgt ggcaaaggtc tgctgcgctg ctaagttgga agatgccgta
     2701 acgcaagcgg ccatccaggg ggagtttcag caccatgctc gatgtcgcct tcgccaagcc
     2761 ggctttgccg aagtctggtg ctgtggtgct gttgattggc gaaggcgaga tgccgtccgg
     2821 cctgtggcaa caagcggatg aggcaaccgg cggcgcgatt gctcgcgcgc tcgcggcggc
     2881 cgaattcaag ggcgcgaagg gcaagacgtg catgattctt gcgccaggtg cgggactttc
     2941 gcgcgtgctg gcggtgggac tgggcaagcc cgcggaattg aagccacttg ggttgaatga
     3001 ggccggcggt gcgattgcgg cggcgatgcc gcgtgaggcg gcggtagcca tagcgaccgg
     3061 cacattgcag ccggcgctgg cggccgaggt ggcattcggt gccgtgctgc gggcgtatcg
     3121 gttcgacaag tatcgcacca aggaaaaagc cgaggacaag ccgaagcttg gcaagatcac
     3181 ggtgctgacg gcggagccgc cgcgcgcgaa agcggcctgg gagccgctgg aaggtgtagc
     3241 gaacggcatc ttcctgacgc gcgacctcgt gagcgagccg cccaatgtgc tgaacccggc
     3301 ggaaatggcg gatcgctgca agaagttgag cgaactaggg ctgaaggtcg aaatcctcgg
     3361 ccctcgggag atgacgcggt tggggtttgg agcgctgctt ggcgttgcgc aaggcagtgt
     3421 gaatgagccc cgcatggtgg tgatgcaatg gaacggcgcg tcgggcagcg ggcgcaagcg
     3481 cggcagtagt ggcaagccgg tcgccttcat cggcaagggt gtgacgttcg atacgggtgg
     3541 gatcagcatc aagccggccg gcggcatgga ggacatgaag tgggacatgg ctggcgcggg
     3601 cactgtcgtc gggctgatgg cggcgctggc cggacgcaag gcgaaggtcg atgcggtggg
     3661 cctggtgggg ctggtcgaga acatgccatc gggcagcgcg cagcgccccg gcgacgtagt
     3721 gaagacatac tcgggccaga cggtcgaggt gatcaacacg gacgccgaag ggcggctggt
     3781 gctggcggat gtgctttggt actgccagca gaaatttgat ccgcgcttca tggtcgatct
     3841 ggcaaccttg accggtgcga tgatcgtggc gcttgggcac gagtatgccg ggatgttcag
     3901 caacgatgat gcgctggcgc agaagctgtc ggcggtcggg agcgacgtga acgagaaggt
     3961 gtggcggttc ccgctgcacg atgagtatga caagctgatc aggtcaaaca tcgccgacat
     4021 gaagaatatc ggcgggcgtc ccgcgggatc gatcagcgcg gcgcaattca tccaacggtt
     4081 cgtcaacaat aagccatggg tgcatctgga cattgccggt atggcctgga gcggcaagga
     4141 caccgcgtgc gtgccgaagg gtgcgactgc ttttggcgtg cgcctgctcg atcggctggt
     4201 ggcgcagcac ttcgaggggt aggggaagtt gtcgttgtga gccgcaggcg aagcaattcc
     4261 gttcgagatt gcttcgcctg tcggcgcgcc atgacaggag tgggcacggg ggtggcgtga
     4321 ccgatattgg tttttaccat ctgacgcgca ccgggccgga caaggcgctg ccgcagctgc
     4381 tggggcgaac gctcgcggca ggtcagcgcg ctgtcgtgtt gtgcgccgat gtggaacgtt
     4441 tggccgcgct ggacgctgcg ctgtggcagt gcgccgaacc cgattggctg ccgcacggca
     4501 cggcggcgga tggtgatgca gatcttcagc cggtctggct caccacggaa gtcgcggcac
     4561 cgaacggcgc gcgcttcctg ttcctgctgg atggcctacg aagcgagcgc ctgggcgagt
     4621 tcgaccgcgt gttcgacctg ttcgatggcc aggatgaagc cgcggtgcag gcggcacgtg
     4681 agcgctggcg cgccgcgaag ggcgcggggc acgaactgac ctactggcaa cagggcgcta
     4741 agggttggga gaagaaggcg taagccgctc gccgagcggc ttcagcacgc ggatccccgg
     4801 tgtcccggca agcagaccag cctgcgatgg aggcatcacc gcgatgccgc ggcggcgtac
     4861 actctgatcg aggccgacag cctccagcca gcgcaccatg gcatcgcgtg gcagcgagcc
     4921 ttcggcgcca cgc
//
LOCUS       DATGEM010000644         7699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_730248, whole genome shotgun sequence.
ACCESSION   DATGEM010000644 DATGEM010000000
VERSION     DATGEM010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730248"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VKQ27_19875"
     CDS             complement(<1..1501)
                     /locus_tag="VKQ27_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY91253.1"
                     /translation="MPFEGIGAAVRRKEDVRFLSGNGNYTDDINRAGQLYAFIRRSDR
                     PHANISRIDTAAAAKMPGVVAVFTGADMAADNVGGLPCGWQIHNKDGSPMQEPAHPVL
                     AVGKVRHVGDPVAVAIAVTKQQAKDAAEAVVIDYQDLPAVASLRDALAPGAPAVHDDA
                     PGNICYDWHIGDKAVVDAAFASAAKVVKLELLNNRLIPNAMEPRAAVGDFDRSNGDYT
                     LYTTSQNPHVIRLLMGAFVLHIPENKLRVVAPDVGGGFGSKIYHYAEEAIVTWAAGKV
                     RRPVKWTAERTESFMSDAHGRDHDSVAEMALDANGKFLALRVSTLANMGAYLSTFAPC
                     IPTYLYATLLAGVYTTPAIYCEVKAVFTNTVPVDAYRGAGRPEATFMLERLVDACARD
                     TGIDRVEIRRRNFIPADAFPYQTPVALQYDSGNYQATLDECLKEADYAGFEARRAAAA
                     AKGKLRGIGVSTYLEACGIAPSAVVGSLGARAGLYECANIKVHPTGSVTV"
     gene            complement(1524..2015)
                     /locus_tag="VKQ27_19880"
     CDS             complement(1524..2015)
                     /locus_tag="VKQ27_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLY91254.1"
                     /translation="MPQVTLTVNGKQHTVEVEGRTLLVELLREKLGLTGTHVGCDTSQ
                     CGACVVHVDGVSAKSCTMLALQADGTDVLTIEGLAEADGTLHPMQEMFREHHALQCGF
                     CTPGMVMSAVDLVNSHPEGLTEKQIRAGLEGNLCRCTGYHNIVKAIAAAWPLMHGKAA
                     AAE"
     gene            complement(2003..2464)
                     /locus_tag="VKQ27_19885"
     CDS             complement(2003..2464)
                     /locus_tag="VKQ27_19885"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY91255.1"
                     /translation="MIAVDTSALIAILFLEPEAEAFQHRLSLEEVCRSAVTLQEASMV
                     LAGRLRDETALEELDDLVRITLIEVVWPNSAAWPSSASARAASGGPEFRRLRLVCAGQ
                     TSRHSAALQGHRSRQDRHRSGVGATRLTRARRGDHTPAHATTLGGIARCLR"
     gene            2871..3737
                     /locus_tag="VKQ27_19890"
     CDS             2871..3737
                     /locus_tag="VKQ27_19890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY91256.1"
                     /translation="MAHNGVSMTDVQLPYIDYLLSELNRRNPTIEKSFGRHVHWGYWT
                     DPRSATNDDNDFARAAEQLTLELCQLAQIADGDSVLDAGCGFGGTIASLNERLHRLQL
                     VGLNIDARQLARASQQVQPLHGNSIEFRQGDACDLPFPSNSFDRVLAVECIFHFPSRK
                     TFFREAARVLKPGGTLTLSDFVPSALFWPICELGAIRGSHRINSIGRWDIRSTLGQYR
                     RLAKRVGLVPSATRNITQHTLPTYRYLQHILARDLPNDREARMFRHLLGLARSLGAIG
                     LMNYDLLSFSKP"
     gene            complement(3867..4706)
                     /locus_tag="VKQ27_19895"
     CDS             complement(3867..4706)
                     /locus_tag="VKQ27_19895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLY91257.1"
                     /translation="MNIDFAAADAGAWSHRNTGFEDALNALSFAFPAGEAYFINSVRY
                     YVNRIKDPVLKEQAERFIYQEAMHSREHDRANHELRQFHAYGHQIERLVMPMLAFSRR
                     CMPKATQLATTCALEHFTAMLADCLLRDQQRFIADSAPSFVALWLWHAVEETEHKAVC
                     FDVYRHVCGKGAFSYLHRVIVMGAVSVLFLGVLGVTFMRMKWSRSSKPERRGGERAPT
                     ARQPGTPTLAYLLHDVPLRMYFDYYRPSFHPWRHDNAHLVDEWKVHFREFGLERDEIR
                     TNR"
     gene            complement(4762..5772)
                     /locus_tag="VKQ27_19900"
     CDS             complement(4762..5772)
                     /locus_tag="VKQ27_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLY91258.1"
                     /translation="MNNQRPLLEVNELKKHFPIHGGILGLETAKVYAVDGVTFSINRG
                     ETLSLVGESGCGKSTVGKAILRLYAPTDGEVYLDGERIDNLSAARLRPMRRRVQVVFQ
                     DPFSSLNPRMRVRDILAEPLINFGIARGGRELTERIEELMAKVQLPRDAIGRFPHEFS
                     GGQRQRIGIARALAPGADLIICDEAVSALDVSVKAQIINLLVDLQDELGLALLFISHD
                     LAIVEHLTHRVAVMYLGKIVELADRRTLFAKSHHPYTRALLSAVPVPDPTAKRHRIIL
                     TGDVPSPINPPSGCRFHTRCPFAFDRCRVEEPVLRTVGEGHVSACHLDHVPGGQVVPM
                     AA"
     gene            complement(5778..6767)
                     /locus_tag="VKQ27_19905"
     CDS             complement(5778..6767)
                     /locus_tag="VKQ27_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91259.1"
                     /translation="MALLEVDNLQTHFGTPDGVVRAVEGVSFHINAGETLGVVGESGC
                     GKSVTAMSILRLIPEPPGKIAGAVRFEGRNLLDVPEDEMRDIRGNAISMIFQEPMTSL
                     NPVLTVGRQIGETLRLHQGLSARDAERKAIDMLVLVGIPAPQRRVREYPHQLSGGMRQ
                     RVMIAMALACNPKLLIADEPTTALDVTIQAQILDLMRDLNKRLGSAIMLITHDLGVVA
                     EMCERVVVMYAGRKVEEAPVEEIFARPLHPYTRGLLGSVPKLGSSLVDGGRSKLAEIE
                     GLVPSLRQRIVGCPFAGRCPMATELCSRVAPAIEAKAPGHLAACHYAERRMAA"
     gene            complement(6769..7680)
                     /locus_tag="VKQ27_19910"
     CDS             complement(6769..7680)
                     /locus_tag="VKQ27_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91260.1"
                     /translation="MSTATTTPSGYAAAPELPDVLPPVKQRGRVATFIRRNPTIVGGG
                     ILMAIMILIAILAPFLGTVDPTALAPVKRLRAPSAQYWFGTDALGRDVYSRVIYGSRV
                     SLIVGFAVATIASGIGTFIGLVSGFVRPLDAVLMRVMDGLMSIPSILLAIALMALLGA
                     SVENVIIAVSVSEFPRVSRLIRGVVLSLREQPFVEAAVASGTRVPKIIWKHILPNTLA
                     PLMVNATFICASAMITEAALSFIGAGTPPIIPSWGNIMAEGRALWQIKPYIVFFPAIF
                     LCITVLSVNLLGDGLRDALDPRLAKRL"
BASE COUNT         1479 a         2679 c         2206 g         1335 t
ORIGIN      
        1 agacagtcac gctgccggtc gggtgcacct tgatgttggc acactcatac aggccggcgc
       61 gcgcgcccag cgaacccacc accgcggatg gcgcgatacc gcacgcctcc aggtaggtgg
      121 aaaccccgat gccgcgcagc ttgcccttag cagcggcagc ggcacgacgc gcctcgaagc
      181 cggcgtaatc cgcctccttc aggcactcgt ccagcgtcgc ctggtagttg ccgctgtcgt
      241 actgcagcgc gaccggcgtc tggtacggga aggcatcagc cgggatgaag ttccgccggc
      301 ggatctccac ccgatcgatc cccgtgtccc tggcacaggc atcgaccagc cgttccagca
      361 tgaacgtcgc ttccgggcgc ccggcaccgc gataggcatc caccggcacg gtgttggtga
      421 acaccgcctt cacctcgcag tagatcgccg gcgttgtata cacgcccgcc agcaatgtcg
      481 catagagata cgtcggtata catggcgcga aggtggacag atacgcaccc atgttcgcca
      541 gcgtcgacac gcgcagcgcc aggaacttgc cgttcgcatc cagcgccatt tcggcgacgc
      601 tgtcgtgatc gcggccatgc gcatccgaca tgaagctctc ggtgcgctcc gccgtccact
      661 tcaccggccg cctcaccttg ccggccgccc aggtgacgat ggcttcctcg gcgtagtggt
      721 agatcttcga gccgaacccg ccgcccacat ccggcgccac cacgcgcagc ttgttctccg
      781 ggatgtgcag cacgaaggca cccatcagca gccggatcac gtgcgggttc tggctggtgg
      841 tgtacagcgt gtagtcgccg ttcgacctgt cgaagtcgcc gaccgcggcg cgcggttcca
      901 tcgcgttggg aatcagccgg ttgttgagca gctccagctt caccactttc gccgcactgg
      961 cgaacgcggc atccaccacc gccttgtcgc cgatatgcca gtcatagcag atgttgcccg
     1021 gcgcatcgtc atgcacggct ggtgccccag gtgcgagcgc atcgcgcaat gacgccaccg
     1081 ccggcaagtc ctgatagtcg atcacaaccg cctcggccgc gtccttggcc tgctgcttcg
     1141 tcactgcgat cgccaccgca accgggtcgc cgacatggcg cactttgcca accgccagta
     1201 ccggatgcgc cggctcctgc atcggcgagc cgtccttgtt gtggatctgc cagccacatg
     1261 gcagcccccc cacattgtca gccgccatat cggcgccggt gaacaccgcc accacgcccg
     1321 gcatcttcgc cgccgcggcg gtatcgatcc tgctgatgtt ggcatgcggc cgatcggacc
     1381 ggcggataaa ggcatagagt tgccccgccc ggttgatgtc gtccgtgtaa ttgccattgc
     1441 cgctgagaaa gcgcacgtcc tccttgcggc ggacggcggc accaataccc tcgaaaggca
     1501 tcgacgttcc tccctggttc gtcttattcc gcagccgccg ccttgccgtg catcaacggc
     1561 caggcggctg cgattgcctt gacgatgtta tggtagccgg tgcatcggca gagattgcct
     1621 tccagaccgg cacggatctg cttctccgtc agaccctcgg gatgcgagtt caccagatcg
     1681 acggcgctca tcaccatccc gggcgtgcag aacccgcatt gcaaggcgtg atgctcccgg
     1741 aacatctcct gcatcggatg cagcgtcccg tccgcttcgg cgaggccctc gatcgtcagc
     1801 acatcggtgc cgtccgcctg caacgccagc atggtgcatg acttcgccga cacaccgtcc
     1861 acgtgcacga cacaggcacc gcattggctc gtatcgcagc cgacatgtgt gccagtcagg
     1921 ccgagtttct cgcgtagcag ctccaccagg agcgtgcggc cctcaacctc caccgtgtgc
     1981 tgtttgccgt tcaccgtcag ggtcacctga ggcatcgcgc aatccctccc aatgtcgtcg
     2041 cgtgggccgg agtgtggtca ccccgccgtg ccctggtcaa gcgggtggcg ccaacgccgg
     2101 aacgatgtcg gtcctggcga gatcggtgcc cttgaagagc agcggaatgt cgcgatgttt
     2161 ggccagcgca tacgaggcgc aatcgccgaa attcagggcc gccggatgcg gcccttgccg
     2221 aagcggagga aggccaggcg gctgaattcg gccagacaac ttcgatcaga gtgatcctga
     2281 ccaggtcgtc cagctcctcc aatgccgtct cgtcgcgcag tcggcccgcc agaaccatcg
     2341 atgcctcctg caaggtcacc gccgagcggc acacttcctc cagggagagt cgatgctgga
     2401 atgcttccgc ttcaggctcc aggaagagaa ttgcgatcag tgcggacgta tcaaccgcaa
     2461 tcacatagga aggccgttct catcataagg atcgtcttcg ctgaagggcg gagcgtccct
     2521 gaatctggcc gcagcacgct cggcaatggc cctgatccgc tgataacgcg cctcaacctc
     2581 ctcgggcgta agggtccgtc gccgcacccc acgctcaacg aattctgcgc ccgctgcttc
     2641 cagtacccgc tggacctcat cgaacgtgcc cggagacacc ttgctcagcc cgtccggcgc
     2701 ctccagccgc cgcatacagt aacgacggag acatgggctc tccacgccaa ctcctcttgg
     2761 tccatacgca gcagggcacg cgcagcccgg atatgatcgg gcgtgatcag catgattaat
     2821 tcacatgatc gcgccttccc gcccttcaag ataaatcgcg aaccgcaaaa atggcgcaca
     2881 atggcgtcag catgaccgac gttcagctcc cctatatcga ctacctcctg tcagagctga
     2941 accgtcgtaa cccgaccatc gagaagagtt tcggcaggca cgtccattgg ggctactgga
     3001 cagacccccg aagcgcgacc aacgatgaca acgacttcgc ccgggcggct gaacaactca
     3061 ccctcgagct ttgccaactc gcccagatcg ccgacggcga cagcgtcctc gatgccggat
     3121 gcggcttcgg cggcaccatt gcgtcgctga acgagcggct tcatcggctt cagcttgtcg
     3181 gcctgaacat cgacgccagg cagttggcgc gcgccagcca gcaggtgcag ccactgcacg
     3241 gcaacagcat cgagttccgc cagggcgacg catgcgatct gcccttcccg agcaacagct
     3301 tcgatcgcgt gctggcggtc gaatgcatat tccacttccc cagccgcaaa accttcttcc
     3361 gcgaggccgc ccgcgtcctg aagcctggcg gcaccctgac gctgtccgat ttcgtcccct
     3421 ccgccctgtt ctggccaatc tgcgaactgg gcgccattcg cggttcgcac cgcatcaaca
     3481 gcatcggcag gtgggacatc cggtccacgc tcggtcaata tcggcgcctg gccaagcgtg
     3541 ttggcctggt gcccagcgca acccgcaaca tcacccagca cacccttccg acgtaccgct
     3601 atctgcagca cattctggcc cgcgacctgc ccaatgaccg cgaagccagg atgttccgtc
     3661 atctgctcgg gttggccaga tcgctcggcg cgataggcct gatgaactac gacctgctgt
     3721 cgttcagcaa accctgatgc cgccgcgcca ggtcaccgcg aattgccacc gaaaccaggg
     3781 gtgaactgag ctagcttcgc ggataccttg tgtccgtgca gcgcagggat ctatttgcgt
     3841 attttgcggt tccattttct gattgatcag cgattcgtcc gaatctcgtc acgctccagg
     3901 ccaaattccc ggaaatgcac cttccattca tcgaccagat gcgcattgtc gtgtcgccac
     3961 ggatggaacg agggtcgata gtaatcaaaa tacatccgca acggcacatc atgcagcagg
     4021 tacgcaagcg ttggcgtgcc tggttgccgg gcagtcggcg cccgctctcc gccacgccgc
     4081 tccggtttcg atgatctgct ccacttcatc cgcatgaacg tgacgcccag cacgccaagg
     4141 aacagcacgc tgacggcgcc catgacgatc acccggtgca gatagctgaa cgcacccttg
     4201 ccgcatacgt gccgatagac atcgaagcag accgccttgt gttcagtctc ctccaccgcg
     4261 tgccagagcc acagcgccac gaatgacggc gccgaatcag caatgaaccg ctgctgatcg
     4321 cgcaacaggc aatccgcaag catcgccgtg aaatgttcaa gcgcacatgt ggtggccagt
     4381 tgcgtcgcct ttggcatgca tcggcggctg aaggccagca taggcataac cagtcgctcg
     4441 atctgatgcc catacgcatg gaactgccga agctcgtgat tggcgcgatc gtgctccctg
     4501 gagtgcatag cctcctgata gatgaaccgc tctgcctgct ccttgaggac cggatccttg
     4561 atccggttca cataataccg gactgaatta atgaaatacg cttcaccagc cgggaacgcg
     4621 aaagacaagg cattcagcgc atcctcgaac cccgtgttcc gatggctcca cgcgccggcg
     4681 tccgccgcgg cgaagtcgat gttcacccgt ctgcgtatga tccgactggg gtccgcacgc
     4741 atggcgcgaa cctaccacag cttacgccgc catgggtaca acttgcccgc cgggcacatg
     4801 atccagatgg cacgccgaca cgtgcccctc gcccaccgtc cgcagcaccg gttcctccac
     4861 acggcagcga tcgaacgcga aggggcagcg cgtgtggaaa cggcagccgc tcggcggatt
     4921 gatcgggctt ggcacatcgc ctgtcaggat aatccggtga cgcttcgccg tcgggtccgg
     4981 caccggcacc gccgacagca gcgcgcgtgt atacggatga tgcgacttgg cgaacagcgt
     5041 gcgccgatcc gccagttcga cgatcttgcc aaggtacatc accgccaccc gatgcgtcag
     5101 atgctcgacg atcgcgaggt cgtggctgat gaacagcaat gccaggccaa gctcgtcctg
     5161 cagatcgacc agcaggttga tgatctgcgc cttcaccgac acatccagcg ccgaaaccgc
     5221 ctcatcgcag atgatcagat cggcacccgg cgccagtgca cgcgcgatgc cgatacgctg
     5281 ccgctgcccg ccggaaaact cgtgcggaaa ccggccgatg gcatcacgcg gcaactgcac
     5341 cttcgccatc agctcttcga tgcgctcagt caactcccgg ccaccgcgcg cgataccgaa
     5401 attgatcaac ggttcggcca ggatatcgcg cacccgcatc cgcgggttca acgaggagaa
     5461 cggatcctgg aacaccacct gcacgcgccg ccgcatcggt cgcaggcggg ccgccgacag
     5521 attgtcgata cgctcgccat ccagatagac ttcgccgtcc gtcggcgcat aaagccgcag
     5581 gatcgccttg cccacggtgg acttgccgca acccgactca ccgaccagcg acagggtctc
     5641 gccgcggttg atggagaaag tcacgccgtc caccgcgtaa accttcgccg tctccagccc
     5701 gagaatcccg ccgtgaatcg ggaagtgctt cttcagctcg ttcacctcga gcagcggacg
     5761 ctggttgttc atcagcatca tgcagccata cggcgttcgg cgtagtggca ggcggccaga
     5821 tgcccgggag ctttcgcctc gatcgccggc gcgacacggc tgcacagctc ggttgccatc
     5881 ggacagcggc cggcgaatgg gcagccgacg atacgttgcc gcaggctcgg caccagaccc
     5941 tcgatctccg ccagcttgct gcgaccgcca tcgaccagcg atgacccaag cttgggcacc
     6001 gacccgagca gaccgcgtgt atacggatgc agcggacgtg cgaagatctc ctcgaccggc
     6061 gcctcctcca ccttgcgccc cgcgtacatc accaccaccc gctcgcacat ctcagccacc
     6121 acgccgagat catgcgtaat cagcatgatc gccgaaccga gccgcttgtt gaggtcacgc
     6181 atcagatcga gaatctgcgc ctgtattgtc acatccagcg ccgtggtcgg ttcgtcggcg
     6241 atcaacagct tgggattgca cgccaacgcc atcgcgatca tgacgcgctg ccgcatgccg
     6301 cccgacagct gatggggata ctcccgcacc cgccgttgcg gcgctggaat cccgaccagc
     6361 accagcatat cgatcgcctt cctctcggcg tcgcgcgcgg acaggccctg atgcaaccgg
     6421 agcgtctcgc cgatctgacg cccgacggtc agcaccggat tcaggctggt catcggctcc
     6481 tggaagatca tggagatcgc attaccgcga atgtcccgca tttcatcttc cggcacgtcc
     6541 agcaggttgc ggccctcgaa ccgcaccgcc ccggcaatct tccccggcgg ttccgggatc
     6601 agccgcagga tcgacatcgc cgtgaccgac ttgccgcatc ccgactcacc cacaacgcca
     6661 agcgtctcgc cggcattgat gtggaacgaa acaccttcga cagcacgcac cacaccgtca
     6721 ggcgtgccga aatgcgtctg cagattatcg acctcgagca gcgccatgtc agagacgctt
     6781 ggccagccgc ggatccagcg catcgcgcag accgtcaccc agcaggttca ccgacaacac
     6841 cgtgatgcac aggaagatcg cagggaagaa cacgatatac ggcttgatct gccagagcgc
     6901 cctgccctcc gccatgatgt tcccccagct tgggatgatc ggcggcgtgc cggcaccgat
     6961 gaacgaaagc gccgcctcgg tgatcatcgc ggaagcgcag atgaacgtcg cgttcaccat
     7021 tagcggcgcc agcgtgttcg gcaaaatatg cttccagatg atcttcggca cgcgcgtgcc
     7081 ggacgccacc gccgcctcga cgaacggctg ctcacgcagg ctcagcacca cgccacggat
     7141 caggcgcgag acacgcggga actcgctgac gctgacggcg atgatcacat tctccaccga
     7201 ggcacccaac agtgccatca gcgcgatcgc cagcaggatc gatgggatcg acatcagccc
     7261 atccattacc cgcatcagaa cggcatcgag cggccggacg aatcccgaca ccaggccgat
     7321 gaacgtgccg atgcccgatg cgatcgtcgc caccgcgaaa ccgacgatga gcgaaacacg
     7381 cgagccgtag atcacgcgcg agtagacatc gcgtcccagc gcgtccgtgc cgaaccagta
     7441 ctgcgccgac ggcgcacgca gccgcttcac cggggccagt gcagtcgggt caacagtgcc
     7501 gaggaacggt gccaggatcg cgatcaggat catgatcgcc atcaggatgc cgccgccgac
     7561 gatggtcgga ttacggcgga tgaacgttgc caccctgccg cgctgtttca ccggcggcag
     7621 cacgtccggc agttcgggtg cggccgcgta gccgctcggc gttgtggtcg cggtgctcat
     7681 ggcgagattc tcatcccct
//
LOCUS       DATGEM010000645         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1415659, whole genome shotgun sequence.
ACCESSION   DATGEM010000645 DATGEM010000000
VERSION     DATGEM010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415659"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..948
                     /gene="sucD"
                     /locus_tag="VKQ27_19915"
     CDS             88..948
                     /gene="sucD"
                     /locus_tag="VKQ27_19915"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLY91261.1"
                     /translation="MSILVDSSTRVIVQGITGREGSFHAEQMKAMGTKVAGGTSPGKG
                     GTTHLGVPVFNTVADAVEATGANASGIFVPPPFAADAIMEAAAAGITLIVCITEGVPI
                     QDMIRVKDFLRGYPGARLLGANCPGLITPGQAKIGIIPQRITNPGYVGLVSRSGTLTY
                     EVGSALSAAGLGQSTIVGIGGDPVLGLTFTDVIQLFEKDPATTHLVVIGEIGGSDEEH
                     AAELLGRGSRLKTVAFISGRTAPEGKRMGHAGAIVSGNRGTAKSKEEAFRRAGIEVAE
                     TTDQIVGMLR"
     gene            945..1376
                     /locus_tag="VKQ27_19920"
     CDS             945..1376
                     /locus_tag="VKQ27_19920"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HLY91262.1"
                     /translation="MSSLPIAITRAVPADRTRDLTILAALVLWLVYTRVFWALQALHL
                     TMPPCPFYFMTGHPCPFCGGTRSFAFMWQGDIANSVRLYPLGPALFAGTFAGVGALIA
                     GLVTRRTWTPRLTNAQWIVLGIGAASLVLFSWALKVFVLGN"
     gene            1420..1845
                     /locus_tag="VKQ27_19925"
     CDS             1420..1845
                     /locus_tag="VKQ27_19925"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLY91263.1"
                     /translation="MRLAALKEAPYAFGSTYENERGHDEKWWRQRLAERTRFVAEIDG
                     QAAGIVSGGDSETEGAAAVTTMWVAPRFRRRGVGDALIKAVLEWSRSAGYTQVLLWVT
                     ERNEQAEKLYERNGFARTGQVSPVRQGEDRIEYEMARRL"
     gene            1856..>2900
                     /locus_tag="VKQ27_19930"
     CDS             1856..>2900
                     /locus_tag="VKQ27_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY91264.1"
                     /translation="MDFNLTQEQQAIRDTCRDFAREVVAPAAEELDRHHKFGYDIVRQ
                     MAELGLFGLPFSEEFGGAGADFLSLCLAIEEISRADAGVGITLEAAVCLGVSPIYEFG
                     TAKQKSELLPDLIAGRKLWAFGLTEPEAGSDAGATKTRAEAKNGKWVINGAKQFITNA
                     GTDISAGVTITAVTGKTNGETEISALLVPMGTPGYHVLEPYRKLGWHSSDTHPLSFDA
                     CEVPAENLLGPRGGGYRQFLHTLTGGRIAIAALSVGVGQACLDAALAYAKERRTFGEP
                     ISKHQAIQFKLADMATETELARTMVYRAASAYSHSDGQLTDEVRMLAAMSKLYASEAS
                     KRAADNAVQIHGGY"
BASE COUNT          547 a          897 c          951 g          505 t
ORIGIN      
        1 gatcctcaaa gagcacggca tcgaacccgg cgccaacgcg tgggaagctg cccagaagat
       61 cgtcgagatg acaaaagcgg gcgtcgcatg agcatcctcg tcgactcctc caccagggtc
      121 atcgtccagg gcatcaccgg ccgcgagggc agcttccacg ccgagcagat gaaggcgatg
      181 ggcaccaagg tggccggcgg gaccagtccg ggcaagggcg gcaccacgca cctgggcgtg
      241 cccgtcttca acaccgtggc agacgccgtc gaagcaaccg gcgcgaatgc ttcgggcatc
      301 ttcgtgccgc ccccattcgc agccgacgca atcatggagg cggccgccgc cggcatcacg
      361 ctgatcgtgt gcatcaccga aggcgtgccg atccaggaca tgatccgggt caaagacttc
      421 cttcgtggct acccgggtgc gcgacttctt ggagcgaact gcccgggcct cataacgcca
      481 gggcaggcca agatcgggat catccctcag cgcatcacca accccggata cgtgggtctg
      541 gtctcacgca gcggcaccct gacctacgaa gttggatcgg cgctctcggc ggctggcctc
      601 ggtcagtcga ccatcgtggg catcggtggc gatccagtgc ttggcctcac gttcactgac
      661 gtgattcagc tcttcgaaaa agaccctgcg acaacccacc tcgtggtcat tggcgagatc
      721 gggggaagcg acgaggaaca tgccgccgag ctgctcggtc gcggatcgcg gctgaagacg
      781 gtggcgttca tttccggccg cacggctccg gaaggcaagc ggatgggcca cgccggtgca
      841 atcgtctcgg ggaatcgcgg aactgccaag agcaaggaag aagcgtttcg ccgggcagga
      901 atcgaggtgg ccgagacaac cgaccagatc gtgggcatgc tgaggtgagc tcgcttccga
      961 tcgcgatcac ccgtgcggta cctgcggaca ggacccgcga tctgacgatc ctcgcggcgc
     1021 tcgtcttgtg gctggtctat acgcgtgttt tctgggcgct tcaggcactc cacctgacga
     1081 tgccgccgtg tcccttctat ttcatgactg gtcatccgtg tcccttttgc ggcggcacgc
     1141 gctcgtttgc gttcatgtgg cagggcgaca tcgcaaactc ggtgcgcctc tatccgctgg
     1201 gcccggcgct gttcgccggc acgttcgcgg gagtgggcgc gctgattgcg ggcctcgtca
     1261 cccgtcgcac gtggacgccg cggctgacca atgcccagtg gatcgtgctg gggattggcg
     1321 ccgcgagcct cgtgctgttc agctgggctc tcaaggtgtt tgtcctcggc aactaacgtc
     1381 cgtcggtttc actcggacga gtgggcgcta ttccgttcat tgcgtctggc cgcgctcaag
     1441 gaagccccat acgcgttcgg ttctacatat gagaacgagc ggggccacga cgaaaagtgg
     1501 tggcgacagc gtctcgctga acggacccgc ttcgtggctg aaatagatgg ccaggcggcg
     1561 gggatcgtca gcggcggcga ttccgaaaca gaaggcgccg cggcggtcac gaccatgtgg
     1621 gtggctcccc gattccgcag gcggggtgtg ggcgatgcgc tgatcaaagc tgtgctcgaa
     1681 tggtcgaggt cagccgggta cacgcaagtc ctcctctggg taacggaacg caacgagcag
     1741 gcggagaagc tatacgagcg caacggcttc gcgcgcactg gacaggtaag cccggtgcgt
     1801 cagggcgagg acagaatcga gtacgagatg gcccgcaggc tgtaggtttt ctctgatgga
     1861 cttcaacctg acccaggaac aacaggcgat ccgcgacact tgtcgcgact ttgcgcgcga
     1921 ggtggtggct ccggcagcgg aagagctcga ccgccaccac aaatttggct acgacatcgt
     1981 ccggcagatg gccgagctgg gtttgttcgg tctgcccttc tcagaggaat tcggcggcgc
     2041 cggggcggac ttcctgtcgc tctgccttgc gattgaagag atatcccggg ccgacgcggg
     2101 cgtcggcatc accctcgaag ccgcggtctg tctcggcgtg tcgccgatct acgaatttgg
     2161 cacggcaaag caaaagagcg agctgctgcc agacctgatt gcaggccgca agctgtgggc
     2221 atttgggctg accgaacccg aagcaggttc agacgcgggc gccaccaaga ctcgcgcgga
     2281 agcgaaaaac ggcaagtggg tgatcaacgg agcgaagcag ttcatcacca acgcggggac
     2341 ggacatctcc gcaggcgtca ccatcacagc ggtcactgga aagaccaacg gcgagacaga
     2401 gatcagcgcg cttctggtgc cgatgggcac acccggctat cacgtgctgg agccgtatcg
     2461 caagctgggc tggcactcgt cggacacgca tccgctcagc ttcgacgcgt gcgaagtccc
     2521 ggccgagaac ctgctcggtc cgcgcggtgg tggttacagg cagttcctgc acacactcac
     2581 cggggggcgc attgccattg cagctctgtc cgtaggtgtt ggacaggctt gtttggacgc
     2641 tgcgcttgcg tatgccaaag agaggcgcac cttcggggag ccgatctcca agcatcaggc
     2701 tatccagttc aagcttgcgg acatggcgac cgagactgag ctcgcccgca cgatggtcta
     2761 ccgcgccgcc tcggcctact cgcactccga cggccagctc accgatgagg tacgcatgct
     2821 cgcagcgatg tcgaagctgt acgcatccga agcatcgaaa cgagcggccg acaacgcggt
     2881 gcagatccac ggcggctacg
//
LOCUS       DATGEM010000646         4397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_643690, whole genome shotgun sequence.
ACCESSION   DATGEM010000646 DATGEM010000000
VERSION     DATGEM010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643690"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VKQ27_19935"
     CDS             complement(<1..318)
                     /locus_tag="VKQ27_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91265.1"
                     /translation="MRWSVSGITISLLLALPVPSRASGEPAIACNAPDEFVTTDVPLA
                     EFGAAIASGGPVNILAVGSATTVGAPSTKDQKPTAIEGGTFPVQMVRSLNAAMPSVRF
                     TLTV"
     gene            complement(325..1485)
                     /locus_tag="VKQ27_19940"
     CDS             complement(325..1485)
                     /locus_tag="VKQ27_19940"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HLY91266.1"
                     /translation="MTLIRSNRSRDLRVDFFRGLALWWIYTDHIPGDILGKYSLQNFA
                     LCDAAEVFVLLAGFGAGIAYGTTMNRQGYLYAAADTLRRAWTLYIAHIFLFVVYAAQV
                     AYSAAALDRMFYLEEARLNVLADDPFRALLEALLLRFQPNLLNILPLYVTLLLMFALA
                     LPLLRWPKLLFLVSGAIYVAVRMTGFNLPSWDDAGGWFLNPLAWQFLFMIGAILAYAP
                     PRMPRVRWPLDTMAAAILVAGVVIIWVIDNHPRILASMPMPVIRFLITQDKTGLHPFR
                     LISILSLTWVVVRIIPREASWLQSRYSWPLVLMGQNSLPVFCCGIFFGFMARLGLETD
                     DSTAMQFVVNVFGVLAMLAVAGLAAWYRGKGRGSAVPRQTVSLPAVARTDTG"
     gene            complement(1575..2309)
                     /locus_tag="VKQ27_19945"
     CDS             complement(1575..2309)
                     /locus_tag="VKQ27_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY91267.1"
                     /translation="MIFAIASGKGGVGKTWFAVTLAHAAARAGRRVLLFDADLGLANV
                     DIQLGLTPDFDLGSVVAGRALLADAVLHHAEGGFDIVAGRSGSGVLSALEPAALERVL
                     AALRHLAERYDVVLLDLGAGLERAVRRMSAWADCLLVLATEEPTSLTDAYAVLKLHAA
                     DKPHGDARVVVNQAATRQAGERTYATLQRACQTFLGRAPACAGVIRRDDRVRDAIRRQ
                     ALLLTRHPNAPAAVDVTAITAALLAA"
     gene            complement(2306..3145)
                     /locus_tag="VKQ27_19950"
     CDS             complement(2306..3145)
                     /locus_tag="VKQ27_19950"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLY91268.1"
                     /translation="MRLKVYRATNMPAAMARVRAELGGDALILSTRRVADGVEITAAI
                     EVEEPAPPEALEPPEAALGGALRYHGLPAGFAPASLTFAALPLETPLLFVGPPGAGKT
                     LTVARLATRLVMAGGQPLVITADGKRAGATEELAAFTKLLGIRLVVACHPVTLGRALA
                     HRDCPVLIDTPGCNPFDPDQLQELSALAATATATPVLVLPAGLDPAEAGEVAHAFASI
                     GARYLVATRMDLTHRLGGVLAAAAAGGLALSEAGVGPGAADGLQPITREWLAARLMTG
                     VPA"
     gene            complement(3174..>4397)
                     /locus_tag="VKQ27_19955"
     CDS             complement(3174..>4397)
                     /locus_tag="VKQ27_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HLY91269.1"
                     /translation="GQLGRSPKPLAMAAGAATVLALLPGLPALPFIALAGLATTGAWL
                     RHKHPTGVQPTSAAAAPVTEQPISETLKMDMIRLELGYGLLALAGGETPRLTEQIKGL
                     RRSIAAEMGFVLPPVRIQDNMQLGADHYSIRVKEIEAGGGNLRPTMLLAMDPRGGTPA
                     LAGEQTREPAFGLPAMWIEPSLKEEALFRGCTVVDPPSVLTTHLTEVVRENMAELLSY
                     AETQKLLDDQPREQQKLIADLIPSQITVGGVQRVLQSLLAERVSIRDLPTILEGIQEA
                     CGGSVRTIPVIVGHVRARLARQISDSYTGPGGYIPLVALSAEWEAAFAEALVGQPEDR
                     QLAMAPSRLQEFMQKLRGAFDAAAGTGETPVLLTSGGIRGHVRAIVERIRPTTPVLAQ
                     AEIWPRARIRTVGTI"
BASE COUNT          801 a         1580 c         1376 g          640 t
ORIGIN      
        1 cacggtcagc gtgaaccgca cggacggcat cgcggcattc agcgagcgga ccatctgcac
       61 ggggaaggta cctccctcga tggcggtggg cttctggtcc ttggtgctcg gcgcgccgac
      121 agtggtcgcg gagccgaccg ccagaatgtt gaccggcccg cccgaggcga tcgccgcgcc
      181 gaactctgcc aatggcacgt cggtcgtcac gaattcatcc ggcgcgttgc aggcgatggc
      241 cggttcgccg ctggcccgcg acggtaccgg caacgcgagc aacaggctga ttgtgattcc
      301 cgagaccgac cagcgcatat acgctcaacc ggtatcagtc cgcgcgaccg ccggcaagct
      361 gactgtttgg cgcggaactg ccgaaccgcg ccccttcccg cgataccagg ccgcaagccc
      421 cgccaccgcc agcatcgcca ggacgccaaa cacgttcacc acgaactgca tcgcggtgct
      481 gtcgtccgtt tccagcccaa gccgcgccat gaagccgaag aagatgccgc agcagaacac
      541 cggcaacgag ttctgtccca tcagcaccag cggccaggaa tatcggctct gcagccacga
      601 cgcctcacgc gggatgatcc gcacgaccac ccaggtaagc gacagaatcg atatcaggcg
      661 gaatggatgc agcccggtct tgtcctgggt gatcaggaac cggatcaccg gcatcggcat
      721 cgaggccaga atgcgcggat ggttgtcgat cacccagatg ataaccacgc cggcgaccag
      781 tatagcagcc gccatcgtat ccaacggcca gcgcacgcgc ggcatgcgtg gcggcgcata
      841 ggccagaatc gcaccgatca tgaacaggaa ctgccaggcg agcgggttca ggaaccatcc
      901 gcccgcgtcg tcccacgaag gcagattgaa gcccgtcatg cgcaccgcga catagattgc
      961 ccccgagacc aggaacagca gcttcggcca gcgcagcaac ggcagcgcca gcgcgaacat
     1021 cagcagcaat gtcacataca gtggcaggat gttcagcagg ttcggctgaa atcgcagcag
     1081 cagagcttcc agcaatgcgc ggaacgggtc gtccgccagc acgttcagcc gcgcctcttc
     1141 cagatagaac atgcgatcca gcgccgcggc ggaataggcc acctgagcgg cataaaccac
     1201 gaacaggaag atgtgcgcga tatacagcgt ccaggcacgc cgcagcgtat cggccgccgc
     1261 atacaggtaa ccctgccggt tcatcgtcgt gccatacgca atcccggcac cgaagccggc
     1321 aagcagcacg aacacctccg ccgcgtcgca cagcgcgaag ttctgcagac tgtatttccc
     1381 cagaatgtcg ccagggatgt ggtccgtgta gatccaccac agcgcaaggc cgcggaagaa
     1441 atccacgcgc aggtcgcggc tgcggttgct gcggatcagc gtcatgctct cctccgagcc
     1501 gcctatgtgg ggctgaattg tgcggagcga aagcgaaacc ccgcgcctgg caggtttcct
     1561 cgcgcgacaa agtgttacgc ggcgagaagc gctgcagtga tggccgtcac atccacggcc
     1621 gccggcgcat tcgggtggcg cgtcagcaac agggcctgtc ggcgaatggc gtcgcgcacg
     1681 cgatcgtcac gcctgatcac accggcgcag gccggtgccc ggccgaggaa ggtctggcag
     1741 gcgcgttgca gcgtcgcata ggtgcgctcc ccggcctggc gcgtcgccgc ctggttcacg
     1801 acgacgcgcg cgtcaccgtg cggcttgtcg gcagcgtgca gcttcagcac ggcataggcg
     1861 tcggtcaggc tggtcggctc ttccgtcgca agcaccagaa ggcaatccgc ccaggccgac
     1921 atgcgccgta cggcccgttc cagtccagcg ccgagatcga gcagcaccac atcgtagcgc
     1981 tccgccaaat ggcgcagcgc cgcgagcaca cgttccagtg ccgcgggctc aagtgccgac
     2041 aatacgccgg aaccggagcg gccggcgaca atgtcgaagc cgccctccgc atggtgcagc
     2101 accgcatccg ccagcagcgc gcggccggca accacgctgc cgaggtcgaa atccggcgtc
     2161 aggccaagct ggatatccac gttggccaac cccagatcgg catcgaacag caacacgcgc
     2221 cgcccggccc gcgcagcagc atgcgccagc gtgacggcga accaggtctt gccgacaccg
     2281 cccttccccg aggcaattgc gaagatcatg cgggcactcc cgtcatcagg cgcgcggcga
     2341 gccactctcg tgtgatcggc tgcagcccgt cagccgcgcc cggccccaca ccggcctcgc
     2401 tcagcgcgag gccaccggcc gctgccgccg caagcacacc gccgagccgg tgcgtgaggt
     2461 ccatccgcgt cgccaccaga tagcgcgcgc cgatcgaagc gaacgcatgg gcgacctctc
     2521 ccgcctcggc cggatcgagg ccggcgggca acaccaggac tggcgtggcg gtggcggttg
     2581 ccgccagcgc ggacagttcc tgcagctggt ctggatcgaa aggattacag ccgggcgtgt
     2641 cgatcaggac aggacagtcg cggtgggcca gcgcgcggcc gagcgtcacc ggatggcagg
     2701 ctaccaccag ccggatcccc agcagcttgg tgaaggccgc cagttcctcc gtggcccctg
     2761 cccgcttacc gtcggcagtt atgaccagcg gctggccgcc tgccattacc aatcgcgtcg
     2821 ccagccgcgc aacggtcaga gtcttgccgg cgcccggcgg ccccacgaac aacagtggcg
     2881 tctccagcgg cagcgcggca aaagtcaggc tcgccggtgc aaaccccgct ggcaggccat
     2941 gataccgcag cgctcctccc agcgccgcct ccgggggttc cagcgcctcc ggcggtgccg
     3001 gctcctcgac ttcgatcgcc gcggtgatct ccacgccgtc agcgacgcgc cgggtggaga
     3061 ggatcagagc gtcgccaccc agctccgccc gtaccctagc cattgccgcc ggcatattgg
     3121 tcgcgcgata gaccttcagc cgcatggtcg atcaggcgcc agggccgccc ccatcatatc
     3181 gtccccactg tgcggatccg cgcacgcggc cagatctctg cctgagccaa tactggcgta
     3241 gtgggccgga tccgctccac gatcgcccgc acatgtccgc ggatgccgcc ggacgtcagc
     3301 agcaccggcg tctcgccagt gcccgccgcc gcgtcgaacg cgccgcgcag cttttgcatg
     3361 aactcctgca acctgctggg cgccatagcc agttgccggt cctccggctg cccgaccagt
     3421 gcctcggcga acgccgcctc ccattcagcc gacagtgcga ccagcggaat gtagccgccc
     3481 ggcccggtgt agctgtcgct gatctgccgt gccagacgcg cacgcacgtg gccgacaatc
     3541 accggaattg tccgcaccga cccgccgcat gcttcctgga tcccctcgag gatcgtcggc
     3601 aaatcgcgga tcgacacccg ctccgccagc aggctttgca gcacgcgctg caccccccct
     3661 accgtgatct gcgacgggat caggtcggcg atcagtttct gctgctcgcg cggctggtca
     3721 tccagcagct tctgcgtctc ggcataggac aacagctcag ccatgttctc gcgaaccacc
     3781 tcggtcaggt gcgtggtcag cacgctgggc gggtcgacca cggtacagcc gcggaacagc
     3841 gcctcttcct tgagcgacgg ctcgatccac atcgccggaa gtccgaacgc cggctcgcgc
     3901 gtctgctcgc ccgccagcgc cggtgtaccg cccctcggat ccatcgccag cagcatggta
     3961 ggacgcagat tgccgccgcc cgcctcgatc tccttcaccc ggatcgaata gtgatcagcg
     4021 ccgagttgca tgttgtcctg gatgcgcacc ggcggcagca cgaagcccat ctcggcggca
     4081 atagaccggc gcagtccctt tatctgctca gtgagccgag gcgtttcgcc acccgcgagc
     4141 gcgagcaggc catagcccag ctcgagccgg atcatgtcca ttttcagcgt ctcactgatc
     4201 ggctgctcgg tcacgggcgc agccgccgcc gatgtgggct gcactccggt cgggtgcttg
     4261 tggcgtagcc aggccccggt cgtggccaaa cctgccagcg cgatgaacgg cagcgccgga
     4321 agaccaggca gcagggccag cacggtcgca gcacccgcgg ccatcgccag cggctttgga
     4381 ctgcggccga gttggcc
//
LOCUS       DATGEM010000647        10063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_844471, whole genome shotgun sequence.
ACCESSION   DATGEM010000647 DATGEM010000000
VERSION     DATGEM010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844471"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VKQ27_19960"
     CDS             <1..1322
                     /locus_tag="VKQ27_19960"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HLY91270.1"
                     /translation="DLAETSDRTGDDDLGKDGELMPTFRGGAAGERSRLFAGIERLGQ
                     QAACAHRLLAAIDLGELHRPSGISRALGPDHADALVRASRQLLQRGLAANIRLLQVSA
                     DCFACILPDGSGDVVETLLQPLLDLFQAPLEHEGIPGMVSPCAGLVRFRAGDAAGRAL
                     LRHAMAAAQDARRLDQMWLLRDATGEDPQRIPSLLRGLRPALAAPDQLSLVYQPRVEM
                     QSGQCHSAEALLRWQHPTLGNVPPAEFIPLVEQTGMIRFVTDWVMEHALAEWSAQPEP
                     ERPRSVSINISPLNLGEENFAGRLGASLDRHGLEPRRVELEFTETAVLRDTGPVTRTV
                     HELAGLGIGIAIDDFGTGYSNLFYLRETPATTVKIDQSFIRPLCDSRRDQIIVQSMIR
                     MAHDLGYRVVAEGIENREVYDMLATWGCDEGQGFHISHPVPIGALGN"
     gene            complement(1336..1941)
                     /locus_tag="VKQ27_19965"
     CDS             complement(1336..1941)
                     /locus_tag="VKQ27_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91271.1"
                     /translation="MSGSKTELEQVGVANSYWGVCGFTSTFAALYKLNSGRKALLHGA
                     GVTTKVLAEIKTYLVTLKAEGKLGLLQEIQTFTRAFPPTAKGTDFSTFTIDSYIDRIN
                     QAVGKSDQELMDEELHSIGMPPRAVVDYLDRMWQRKATLSLFETGANGIIGVKRDNRP
                     MYGGLCHYMYRYGNKIYSWGNTYSSVKDANNAYSVILVISF"
     gene            complement(2033..2458)
                     /gene="rbfA"
                     /locus_tag="VKQ27_19970"
     CDS             complement(2033..2458)
                     /gene="rbfA"
                     /locus_tag="VKQ27_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HLY91272.1"
                     /translation="MLRDGDGTGLSRHDVAGPTQRQLRVAEEIRHVLAGLFERRDFRD
                     PDLAEAEITVAEVRVSPDLKHAIAFVSRLGRTDVDALLPALKRAAPYLRGQVAHALRL
                     KFAPDLTFQPDHALDYATKIDRLLHQPNVARDLERPTET"
     gene            complement(2428..5082)
                     /gene="infB"
                     /locus_tag="VKQ27_19975"
     CDS             complement(2428..5082)
                     /gene="infB"
                     /locus_tag="VKQ27_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HLY91273.1"
                     /translation="MSETNDQDAGKGRLSLRPAGRLELGRTVDAGSVRQSFSHGRSKV
                     VQVEVRKKRAPVGPGGGAPAAPATTAPAARGPAPAERPAVTTVPRALTATETAARQRA
                     LVEQQREAAKREAERREQEKISILSAAEEARRREEEAKKAAEDEARRTAEEAVRQEAE
                     EAARQAAEAVAAAQAAAAAPTTARQAEEARQKPTAAPPQQAPAATPSTPTETLRLRAR
                     GAEEEDEARPIRRPGGVVAPRKPQTVVAKKGTDARRAGRIDVQAAIEGEDERVRSLAS
                     VRRQRERERRQAELERLRSDQVKVVRDVILPDTITVQELANRMAARTADAVKSLMKLG
                     VMATATHVLDADTAELVVQEFGHRARRVSESDVELGLEGSLDTDAELKQRPPVVTIMG
                     HVDHGKTSLLDALRSTDVAAGEAGGITQHIGAYQVQMPDGAKITFIDTPGHEAFTAMR
                     ARGASVTDIVVLVVAADDGVMPQTIEAIRHAKAANAPVIVAINKMDKPDANPSRVRQE
                     LLSHEIVVEEMGGETQDVEVSATQKLGLDNLTLAITLQAEILDLKANPERPAEGTVIE
                     SRLDRGRGPVATILVQKGTLKQGDIIVAGAEWGRVRAMLDDKARALSSAGPSTPVEIL
                     GLAGVPSAGEPFVVVDSEGRAREITEFRTRKLREKAAAATGGVRGTLDQMLARIQAGE
                     QKEVAVLIKADVQGSAEAIQQTVLKLAHEEVKVRVLHAAVGQITESDVQLAKASDAYV
                     LAFNVRATSQARDLAQKEGVDIRYYSIIYEVSDDIEQLVRGKVAPKARERFLGYAEVR
                     KVFSITRVGKVAGCMITEGLVKRGAGVRVLRENVVIHNGELSQLKRFKDDVREVARGY
                     ECGLSFANFHDLQEGDVVECFETEMVPA"
     gene            complement(5083..5811)
                     /locus_tag="VKQ27_19980"
     CDS             complement(5083..5811)
                     /locus_tag="VKQ27_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLY91274.1"
                     /translation="MAGPDPVIGCGRVTAEVDGRVRPGHDDGADDEPEAGPLRRCVVS
                     RERLPKERMIRFVVGPDRVLVPDLAATLPGRGMWLSASWDVIQAARAQGGLGRAFARA
                     ARGPVTVPPDLPAVLEAALVRRIGELLGLARRAGQAVAGFDKAREWLRSGRGRLILQA
                     SDGSAAERARFLAGAADPVTVLDPLPAAVLGQMFGRDHVVHVAIASGRLGERLAVEAE
                     RLRGLRNRGPETSPVARLDGANER"
     gene            complement(5849..7378)
                     /gene="nusA"
                     /locus_tag="VKQ27_19985"
     CDS             complement(5849..7378)
                     /gene="nusA"
                     /locus_tag="VKQ27_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLY91275.1"
                     /translation="MDTAVARPEFLLVADAVAREKNIEREEVLEAMEQAIQKAGRAKY
                     GHEKDIRATIDRKTGDVRLSRWTEAVETVENEETQIPVNIARKFKPDINVGEFLVDPL
                     PPIDFGRIAAQTAKQVIVQRVREYERKKQFEEFKDRVGEIINGVVKRTEYGNLMVELG
                     RAEALLRRDELIPRESFRNGDRVRAYIYDVREEPRGPQIFLSRTHPGFLAKLFAQEVP
                     EIYDGIIEIKAVARDPGSRAKMAVISRDSSIDPVGACVGMRGSRVQAVVQELQGEKID
                     IIPWSPQAATFVVNALAPAEVTKVVMDEEAGRVEVVVPDEQLSLAIGRRGQNVRLASQ
                     LTRWDIDILTEAEESERRQEEFRRRSSLFVEALDVDDVIAGLLVTEGFTSVEELAFTP
                     LEEVGAIEGFDESVAQELIRRAESFITRRDTELTEKRVELGVSDEVAAIEVLTPAMLV
                     ALGEKEVKTLDDLGDLASDELIEIVGADNMDEETANNVIMAARAHWFDEEPTAQEASN
                     D"
     gene            complement(7394..7915)
                     /gene="rimP"
                     /locus_tag="VKQ27_19990"
     CDS             complement(7394..7915)
                     /gene="rimP"
                     /locus_tag="VKQ27_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLY91276.1"
                     /translation="MDTPEAAPTSLEARLVAIIAPTLESMGYELVRVSVLGRERPTVQ
                     VMADRADGSQITVQDCEYISHTLSAVLDVEDPIPGAWSLEISSAGIDRPLTRAKDWNR
                     FAGHLARAETVAPVDGRRRFSGVVLGADDTEARMRLDDGSEIVLPMNTIRRAKLVLTD
                     ALIEATATPPRPN"
     gene            8112..9275
                     /locus_tag="VKQ27_19995"
     CDS             8112..9275
                     /locus_tag="VKQ27_19995"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLY91277.1"
                     /translation="MSLDIAEHPASPQELWRLNRILRIVSAGDEMLIRVDSESALLQG
                     MCQVVVDGGYPMAWIGYAERDEAKTVRPMAWAGSTGAEIQFARCSWADTEYGQGPAGI
                     AIRTGQPQIIQDVATSQCPQAWQDAVIRRGLLSAAAFPLRGPNGAFGAFCIYGHTTET
                     FDPSELELLGKLANDLAFGIKAQRDHLATQEHAQRLQRTLEATVQALANTVEHRDPYT
                     AGHQRNVAALAAAIAQEMGLPNDDVAGIYLAGMIHDIGKIRVPAEILSNPGRLTNIEY
                     QLLQAHPQIGFDIVKSVDFPWPVAKMILQHHERLDGSGYPNQLRGDQIIIGARIIAVA
                     DVVEAMTARRPYREALGLDVALHEIEQGRGRLYDPASVDICIKLFRERRFSLT"
     gene            complement(9397..>10063)
                     /locus_tag="VKQ27_20000"
     CDS             complement(9397..>10063)
                     /locus_tag="VKQ27_20000"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HLY91278.1"
                     /translation="ECPCGPVDVHVTDNGATVAFDSVPAFAAELDTEVDVAGYGRVRC
                     DIGYGGAFYAMLPASRLGLDLLVAPVGQLRAAALAIMRAIRARGAVQHPTEPDLSFLY
                     SVLLTDDTSPNSPYPTRNLCVFGDGQIDRSATGSGVTARMAVDAARGLIAPGGRRSFG
                     GATGVPFHAELLGEEPLGPHRAWRVRVSGRASYSGTATWLVDEADALRDGFAIEEGDR
                     LPG"
BASE COUNT         1724 a         3458 c         3083 g         1798 t
ORIGIN      
        1 atgatctggc tgaaacaagt gaccgcacgg gagacgacga cctgggcaag gacggggaac
       61 tgatgcccac ttttcgtggg ggcgccgctg gcgagcggag ccgcttattt gcgggaatcg
      121 agcggctcgg ccaacaagcc gcctgcgcac accggttgct cgctgcgatc gacctgggag
      181 agctgcatcg gccaagtggc atcagtcgcg cgctggggcc ggatcacgct gatgcgctgg
      241 tgcgcgcatc gcgccagctt ttgcagcgcg gcctggcagc gaacatacgc ctgctccagg
      301 tgagtgccga ctgcttcgcc tgcattctgc cggacggtag cggcgatgtg gttgagacgc
      361 tgctacagcc cttgctggac ctgttccaag cacctctgga gcacgagggc atccccggaa
      421 tggtctcgcc gtgtgccggc ctggtgcggt tccgagccgg cgacgcggcg ggacgcgctc
      481 tgctgcgcca cgccatggcg gccgcgcagg acgcccgccg tctcgaccag atgtggctgc
      541 tgcgcgacgc gacgggcgag gaccctcagc gtatcccatc gttgctccga ggattgcgcc
      601 cagccctcgc cgcgccggat cagctgtcgc tggtgtacca gccaagggtc gaaatgcaat
      661 ccggccagtg ccatagcgcc gaggccctgc tgcgctggca gcacccaacc cttggcaacg
      721 taccaccggc cgagttcatc ccgcttgtcg aacagaccgg catgatccgt ttcgtgaccg
      781 attgggtgat ggaacacgcg ctcgccgaat ggtcggccca gccggaaccg gagcggccac
      841 gcagcgtgtc gatcaacatc tcccccctca acttgggcga agagaacttt gccgggcggc
      901 tcggcgcgtc gctggaccgg catggcctgg aaccacgtcg ggtcgagttg gaattcactg
      961 aaaccgccgt cctgcgcgac accgggccag tcaccagaac cgtgcacgaa ctggctgggc
     1021 tcggtatcgg catagcgatc gacgatttcg gcaccggata tagcaatctg ttctatctgc
     1081 gcgagacgcc cgccacgacg gtgaagatcg accagagctt catccggcca ctatgcgaca
     1141 gccgacgcga tcagatcatc gtgcaatcaa tgatacggat ggcgcacgac ctcggctacc
     1201 gcgtggtggc cgagggcatc gaaaatcgcg aggtctacga tatgctggcg acatggggat
     1261 gcgatgaggg acaaggtttc cacatctcgc acccagtgcc catcggcgcg ctggggaact
     1321 gacgccagcg ggcgatcaga acgagatgac caggataacg ctataggcgt tgttggcatc
     1381 cttcacgctg gaatacgtgt tgccccagct gtagatctta ttcccgtatc gatacatata
     1441 atgacagagg ccgccataca tcggcctgtt gtcgcgcttt acgccgatga taccgttcgc
     1501 gccagtttca aataatgaca gggtcgcttt cctctgccac atgcggtcca ggtaatccac
     1561 aaccgcacgg ggcggcatac caatcgagtg gagttcctcg tccatcaact cctgatcact
     1621 cttgcccacc gcttgattga tacggtcgat atagctgtcg atggtgaatg tgctgaagtc
     1681 ggtacctttg gcagtgggcg gaaacgcccg ggtgaaggtc tggatctcct ggagcagtcc
     1741 aagcttgcct tcagccttaa gggtcacgag gtaggtcttg atctccgcta gtaccttggt
     1801 agtgaccccg gcaccatgga ggagcgcctt cctgccgctg ttcagcttat agagcgcggc
     1861 gaacgtgctg gtgaacccgc agacacccca gtagctattg gcaacgccaa cttgttcgag
     1921 ctctgtcttg ctaccggaca ttgtcgtttc cccaagcgtt ggttccaccg gtggtttcag
     1981 aggcgcagcg gactatgtca aggcccgcta gccttgcggc ggaatcgtct gctcaggtct
     2041 cagttggccg ctccaggtcg cgtgcaacat tcggctggtg cagcagacga tcgatcttcg
     2101 tcgcgtaatc gagcgcatgg tctggctgga acgtcaggtc cggcgcgaac ttgagccgca
     2161 gcgcgtgcgc aacctgcccc cgtagatacg gtgcggcgcg cttcaatgcc ggcaagagcg
     2221 catccacatc cgtccgaccc agccgcgaca cgaacgcgat ggcgtgcttt aggtcagggg
     2281 agacgcgcac ctcagcgact gtgatctcag cttctgccag atcaggatcg cgaaagtctc
     2341 gccgttcgaa cagcccagcc agcacatggc ggatttcctc cgccacacgc aactgacgct
     2401 gcgtcggacc ggccacatcg tgcctgctca agccggtacc atctccgtct cgaagcactc
     2461 aaccacgtcg ccttcctgca ggtcgtggaa gttcgcgaac gacaggccgc actcatagcc
     2521 acgcgccact tcacgcacgt cgtccttgaa gcgcttcaac tgcgacagtt caccgttgtg
     2581 gatcaccacg ttctcgcgca gcacacgcac gcccgcgccg cgcttgacca gaccctcggt
     2641 gatcatgcag ccggcgacct tacccacgcg ggtgatgctg aacaccttgc ggacctcggc
     2701 atagccaagg aacctttcgc gagcctttgg cgccaccttc ccccgcacca actgctcgat
     2761 atcgtccgac acttcgtaga tgatcgagta gtagcggatg tcgacacctt ccttctgcgc
     2821 caggtcgcgc gcctgcgacg tcgcacgcac gttgaacgcc agcacataag cgtcggaagc
     2881 cttcgcaagc tgcacatcgc tttcggtgat ctggcccacc gcggcatgga gcacacggac
     2941 cttcacttcc tcatgcgcga gtttcagcac cgtctgctgg atcgcctcgg cgctaccttg
     3001 cacgtcggcc ttgatcagca ccgcgacttc cttctgctcg cccgcctgga tgcgggccag
     3061 catctgatcc agcgtgccac gtacgccgcc ggtcgccgct gctgccttct cgcgcaactt
     3121 gcgggtgcgg aactccgtga tctcgcgggc gcggccttca ctgtccacca ccacgaacgg
     3181 ctcgcccgcc gagggaacgc cggcaaggcc caggatctcc accggcgtgg atggcccagc
     3241 cgacgacagc gcacgggcct tgtcgtcaag catggcgcgg actcggcccc attcggcgcc
     3301 ggccacgatg atatcgccct gcttcaacgt ccccttctgc accaggatcg tcgccacagg
     3361 accacggccg cgatcgaggc ggctttcgat caccgtgcct tcggcggggc gctccgggtt
     3421 cgccttcagg tcgaggattt cggcctgtag cgtgattgcc aaggtcaggt tgtcgagccc
     3481 cagcttctgg gtcgcggaca cttccacgtc ctgcgtctca ccgcccatct cttccaccac
     3541 gatctcgtgg ctgagcagct cctggcggac ccggctcgga ttggcgtccg gcttgtccat
     3601 cttgttgatc gccacgatga ccggcgcatt ggccgccttg gcgtggcgga tcgcctcgat
     3661 cgtctgtggc atcacgccgt cgtctgcggc aaccaccagc acgacgatat cggtcacgga
     3721 cgcgccgcgg gcgcgcatgg cggtgaacgc ctcgtggccc ggcgtgtcga tgaaggtgat
     3781 cttcgcgcca tccggcatct gcacctggta ggcaccaata tgctgggtga tgccgcccgc
     3841 ctcgccggcc gccacgtcgg tggagcgcag tgcgtccagc aacgaggtct tgccgtggtc
     3901 gacgtggccc atgatggtca cgacaggcgg gcgctgcttc agctcggcgt cagtatcaag
     3961 cgagccttcc aggccaagct cgacgtcact ttcagagacg cggcgagccc gatggccgaa
     4021 ctcctgcacc accagttcgg cagtgtcggc atcgaggaca tgggtcgcgg tcgccatgac
     4081 accgagcttc atcagcgact tcaccgcgtc ggcggtgcgt gccgccatac gattggccag
     4141 ctcctgaacg gtgatggtgt caggcaggat aacgtcacgc acaaccttca cctggtcgga
     4201 acgcaggcgt tccagctctg cttggcgccg ctcgcgctca cgctgacggc gcacagaagc
     4261 aagcgagcga acgcgctcgt cctcgccctc gatcgccgcc tgcacgtcaa tcctgccggc
     4321 acggcgcgcg tcagtgccct tcttcgctac caccgtctgc ggcttacgtg gggcaaccac
     4381 accgccaggc cggcggatcg ggcgcgcctc gtcttcctcc tccgcaccac gggctcgcag
     4441 gcgcaacgtc tcggtgggtg tgctgggcgt ggcggcaggt gcctgctgtg gcggcgcggc
     4501 agtgggcttc tgtcgcgcct cctcagcctg acgggcagtc gtcggcgccg cagctgccgc
     4561 ttgggctgcc gcgacggctt cggccgcctg ccgagcggcc tcttccgctt cctggcgcac
     4621 agcctcttcg gcggtgcggc gcgcttcgtc ctcggctgcc ttcttggctt cttcctcgcg
     4681 ccgacgcgcc tcctcggcgg cggagaggat ggagatcttc tcctgctcgc gacgctctgc
     4741 ttcacgcttc gcggcttcgc gctgctgctc gaccagcgcc cgttggcgcg cggccgtctc
     4801 ggtcgcggtc agggcacggg ggacggtggt aacggccggg cgttcagccg gcgctggacc
     4861 gcgtgcggcc ggcgccgtcg tcgccggtgc ggcaggcgca ccgccacctg ggcccaccgg
     4921 ggcacgcttc ttgcgcacct cgacctgcac caccttggag cggccgtgcg aaaaactctg
     4981 ccgcacagag ccggcatcga cagtgcggcc tagctccagg cggcccgctg gccgcagaga
     5041 gagccgaccc ttgccggcgt cctgatcgtt cgtttcactc atctagcgct cgttggcccc
     5101 atccaatcgt gcgaccggag atgtctccgg tcctctgttc cgtagtccac gcaatcgttc
     5161 cgcctcgacc gcaagccgtt cgcccaggcg tcccgacgcg atcgcgacgt gtactacatg
     5221 gtcacgcccg aacatttgac caagcaccgc agccggaagg ggatccaaca ctgtgactgg
     5281 atccgccgct ccggcgagaa agcgcgcccg ctcggccgcg ctgccgtccg atgcctggag
     5341 gatcagccgt ccccgaccac tccgcaacca ctcacgcgcc ttgtcgaacc ccgcgacagc
     5401 ctgccccgca cgcctggcga gccctagcaa ctcgccaatc cgccggacca gcgccgcttc
     5461 gagcacggca ggaagatctg ggggcacagt gacaggaccg cgcgcggccc tggcgaaggc
     5521 acgccccaac cctccctggg cgcgggcagc ttgtatcaca tcccaggagg cactcaacca
     5581 cattccccgt cccggcagcg ttgccgcaag gtccggcaca agcacccggt ccggtccaac
     5641 gacgaagcgg atcatgcgtt ccttgggcag ccgctcgcgc gagacgacgc accggcggag
     5701 tgggccggcc tcaggttcgt cgtccgcccc atcgtcatgg ccgggcctga cccgcccatc
     5761 aacctcggca gtcacccggc cgcagccgat gaccgggtca ggcccagcca tgacggttgg
     5821 gcggccgttc agtgggatgt tcgcgggatc agtcgttgct ggcctcctgc gcggtcggct
     5881 cctcgtcaaa ccaatgggca cgggccgcca tgatcacatt gttggcggtt tcctcgtcca
     5941 tgttgtcggc accgacaatc tcgatcagct catccgaggc aaggtcgccc aggtcgtcca
     6001 gcgtcttcac ctccttctcc ccaagagcca ccaacatcgc tggtgtcagc acctcgatcg
     6061 cggccacctc atcggagacg ccgagctcca cgcgcttctc cgtcagctcg gtgtcccggc
     6121 gcgtgatgaa agactcggcg cgccggatca gctcctgcgc caccgactcg tcaaacccct
     6181 caatggcgcc gacttcctcc agtggcgtga acgccagctc ctcgaccgag gtgaagccct
     6241 cggtcaccaa caaaccggcg atcacatcgt ccacatccag cgcctcgacg aacaggctgc
     6301 tccggcgacg gaactcttcc tgccggcgct cgctctcctc ggcctccgtg aggatgtcga
     6361 tgtcccaacg ggtaagctga ctcgccagcc gcacgttctg gccgcgccgc ccgatcgcca
     6421 gcgacaactg ctcgtccggc accacgactt cgacgcgacc ggcttcctcg tccatcacca
     6481 ccttggtcac ttcggcggga gccagcgcat tcaccacgaa cgtggcggcc tgaggcgacc
     6541 acgggatgat gtcgatcttc tcgccctgca gctcctgcac taccgcctgc acgcgcgaac
     6601 cacgcatgcc aacgcaggca ccgaccggat cgatcgacga gtcgcggctg atcaccgcca
     6661 tcttggcgcg gctgccggga tcgcgcgcca ccgccttgat ctcgatgatg ccgtcgtaga
     6721 tctccggcac ttcctgcgcg aacagcttgg ccaggaagcc gggatgcgtg cgcgacagga
     6781 agatctgcgg gccgcgcggc tcctcgcgca cgtcgtagat ataggcgcgc acccggtcgc
     6841 cattgcggaa gctctcgcgc gggatcagct cgtcgcggcg cagcagcgcc tcggcgcggc
     6901 cgagctccac catcaggttg ccgtactcgg tgcgcttcac cacgccgttg atgatctcgc
     6961 cgacccggtc cttgaattcc tcgaactgct tcttgcgctc gtattcccgc acgcgctgca
     7021 caatcacctg cttggcggtc tgcgcggcga tgcggccgaa atcgatcggc ggcagcgggt
     7081 ccaccaggaa ctcgcccacg ttgatgtccg gcttgaactt gcgagcgatg ttgacgggga
     7141 tctgcgtttc ctcgttctcc accgtctcga ctgcctcggt ccagcgcgac aggcgaacgt
     7201 cgccagtctt gcggtcaatc gtggcgcgaa tgtccttctc atggccgtac ttcgcgcggc
     7261 cggccttctg gatggcctgt tccatcgcct ccagcacttc ctcgcgctcg atgttcttct
     7321 cacgcgcgac agcatcggcg accagcagga attcgggacg ggcaactgcg gtgtccatgt
     7381 ggacctcttg cttctagttg ggacggggtg gggttgcggt ggcttcgatc agcgcgtcgg
     7441 tcaacaccaa tttggcacgc cggatcgtat tcatcggcag cacgatttcg ctaccgtcat
     7501 cgaggcgcat acgcgcctcc gtgtcatcgg cgcctagcac gacgccggaa aagcggcggc
     7561 gaccatctac cggcgccacg gtttcggcgc gcgccagatg acccgcgaag cggttccagt
     7621 ccttcgcacg ggtcagcgga cgatcaatgc ccgccgagga gatctccagc gaccaggcac
     7681 cggggatcgg gtcttcgacg tcgagaaccg cgctcaacgt atggctgatg tactcacagt
     7741 cctggaccgt gatctgagac ccatcggcgc ggtccgccat cacctggacg gtcggacgtt
     7801 cgcggccgag cacggacacg cgcaccagtt cgtatcccat gctttccagc gtgggagcga
     7861 tgatggcgac cagccgggcc tcgaggctgg tgggggctgc ttcgggtgta tccaaaacaa
     7921 aaaaggcggc cggttagggc caccctccgt aactctgcgg aagattgatt tgaacccagg
     7981 agatagagcg tcacgacatc gtttgcaagc gggcaccctc tgcacgcagt gtgtcgcagc
     8041 tgacgactta accggatggc aagctgcttc tgctaggtac ggtgccactc catcgcgcag
     8101 cgtaacagcc cgtgtctctt gatatcgccg agcatccagc atcgcctcag gagttatggc
     8161 gcctgaaccg gatccttcgc atcgtcagcg ccggcgatga aatgctgatc cgcgtcgata
     8221 gcgaatccgc gctcctccag ggaatgtgcc aggtcgtggt cgacgggggc tacccaatgg
     8281 cgtggatcgg ctatgccgag cgcgatgagg caaagacagt acgccccatg gcatgggcgg
     8341 gttcgactgg cgccgagatc cagtttgccc gctgcagttg ggccgacaca gagtacggcc
     8401 aaggccccgc cggaattgca atccgtacag gacagccgca gatcatccag gacgtggcaa
     8461 cctcgcagtg cccgcaggcc tggcaggatg cggttatccg ccgaggcttg ctttccgccg
     8521 cggccttccc gctccgtggc ccgaacggtg cgttcggcgc cttctgcatc tacgggcaca
     8581 cgacggagac attcgatccg tcagagcttg agcttctggg gaaactcgcc aacgacctcg
     8641 cttttggcat caaggctcag cgcgaccatc tcgcgacgca agagcatgcc cagcggctgc
     8701 agcggacgct tgaggcaacg gtgcaggctc tggccaacac ggtcgagcac cgcgatcctt
     8761 acacggcggg acaccagcgg aacgtcgccg ccctcgccgc cgccatcgcc caggagatgg
     8821 gcctgccgaa cgacgacgtg gccggcatat acctagccgg aatgatccac gacatcggca
     8881 agatccgagt gccggcggaa atcctgagca acccggggcg gctgaccaat atcgaatacc
     8941 aactgcttca ggcacatccc cagatcggct tcgacatcgt gaaatcggtg gatttcccct
     9001 ggccggtcgc caagatgatc ctgcaacacc atgaacggct ggatggctcc ggctatccca
     9061 atcaactccg tggcgatcaa atcatcatcg gcgcccgcat tatcgcggtc gccgatgtgg
     9121 ttgaagcaat gacggcgcgt cggccctacc gcgaggcgct cggcctcgat gtggccttgc
     9181 atgaaatcga gcagggccgc ggccgcctct atgatcctgc atccgtcgat atctgtatca
     9241 agcttttcag ggagcggcgg ttctcgctga cttagttaga gcgtgatgac cagaggcgga
     9301 atcgccggag gtctgaatca cgcgataatg caaaaggcca gcgcgagatc agcgcgtcct
     9361 tcagcgatcg tcccgctcta gcacgaagca ctcagcctag ccggggagac gatccccttc
     9421 ctcgatcgcg aatccatctc tcagggcgtc agcctcatcg acgagccacg tggcggtgcc
     9481 gctgtacgaa gccctgcccg acacacggac ccgccaggcc cggtgcgggc cgagcggctc
     9541 ctcgcccagc aactccgcgt gaaacggcac gcctgtggcg ccgccaaagg agcggcgccc
     9601 gccgggtgcg atcaggccac gcgccgcatc gactgccatg cgcgccgtga cgccgctgcc
     9661 ggttgcgctc cggtcgatct ggccatcgcc gaagacacag agattgcgcg tgggatacgg
     9721 cgagtttggc gatgtgtcgt cggtcagcag cacgctgtaa agaaacgaca gatccggctc
     9781 ggtcgggtgc tgcaccgcgc cgcgtgcgcg gatcgcgcgc atgatcgcca gcgccgccgc
     9841 gcgcagttgt cccaccggcg ccacaagcag atcaaggccg agccgcgagg caggcagcat
     9901 ggcatagaat gcgccgccat agccgatgtc gcagcggacg cggccgtagc cggcgacgtc
     9961 gacctcggta tcgagttccg ccgcaaaggc cggaacgctg tcgaaggcga ccgtggcccc
    10021 gttatccgtc acatgcacgt ccaccgggcc gcatggacat tcc
//
LOCUS       DATGEM010000648         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_735544, whole genome shotgun sequence.
ACCESSION   DATGEM010000648 DATGEM010000000
VERSION     DATGEM010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735544"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VKQ27_20005"
     CDS             complement(<1..345)
                     /locus_tag="VKQ27_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HLY91279.1"
                     /translation="MTRNTALREAVPLPDALAGRSIVLVGLMGAGKTSIGRRLAARLG
                     LPFRDADAEIELAAGCTIPELFSRYGEPDFRAGERRVIRRLLAGDPLVLAFGGGAYMD
                     QETRTVAREEAVT"
     gene            413..556
                     /locus_tag="VKQ27_20010"
     CDS             413..556
                     /locus_tag="VKQ27_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91280.1"
                     /translation="MIRIFLIVLAAGVIILAAAVVFLGENPPTPVSHPVEKVLPNDKF
                     QSH"
     gene            557..1447
                     /locus_tag="VKQ27_20015"
     CDS             557..1447
                     /locus_tag="VKQ27_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLY91281.1"
                     /translation="MDRHIESFLEMLAAERGAARNTLMAYQADLEDFGAFAAGKGHLV
                     ASCDAAVLQAYMAGLQRAGLAARTAARRLSALRQFHRFLLREGIRTDDPTTLLDAPRL
                     PKTLPKYLTEQEVDALLGACEPMAGQRGLVARAALEILYATGLRVSELLSLPRQALGG
                     DATVLLIRGKGGKERIVPLSDRAKIAAEALVAAQKKATRYLFPGRAANRALTRQGFFL
                     LLKQVALEAGIDPGRISPHVLRHSFASHLLARGADLRSLQLLLGHADIATTQIYTHVL
                     AERLQRLVEAHHPLAHGETE"
     gene            complement(1478..>2499)
                     /locus_tag="VKQ27_20020"
     CDS             complement(1478..>2499)
                     /locus_tag="VKQ27_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91282.1"
                     /translation="TVVLALILLLLGAAGSSFAHTPWELYATCGVLVGVGSGAGMVGL
                     ATAVANRWFVAHRGLVVGLLTASNASGQLIFLPLLASLASHLGWQSVPWAVAAVLLVL
                     IPLVLLMLAESPGSVGLGPYGAAAEPPLAPSAVNPFRVALSGLARGARSVDFWLLFAT
                     FWVCGFSTNGLVATHLIPFCMDHGIPEVQAASLLAAMGVFDLIGTTLSGWLTDRFNAR
                     VLLFWYYGLRGLSLVLLPFTNFDMLSLSVFAVFYGLDWVATVPPTVGLTTEVFGKRDS
                     PVIVSWIVCGHQMGGAIAALGGGLARNATGSYLAAFIASGAACLLASLMVLRIARRPA
                     VVLAE"
BASE COUNT          467 a          781 c          873 g          378 t
ORIGIN      
        1 ggtgacggcc tcttcgcgcg ccactgtcct ggtttcctga tccatgtatg cgccgccgcc
       61 gaatgccagg accagtgggt cgccagccag cagtcgacga atcacccggc gttcgccggc
      121 gcggaagtcc ggctcgccat agcgtgaaaa cagctccggg atggtgcagc cggcggcgag
      181 ttcgatctcg gcgtcggcgt cgcgaaacgg caacccaagg cgcgcagcga gtcgacggcc
      241 tatcgaggtc ttgccggcgc ccatgagccc gaccagcaca atgctccggc cggcgagtgc
      301 gtccggtagc ggaacggcct ccctcagtgc ggtgttgcgt gtcatggcgg cggacgctag
      361 cacaccgcac accggacagg ccaaggtggc gcaggagtcg gtgagccagt cgatgatccg
      421 catctttctc atcgtcctgg ccgcgggggt gatcatcctc gcggccgcgg tggtgttcct
      481 cggggaaaat ccaccaacgc cggtcagcca tccggtggaa aaagtactgc cgaacgacaa
      541 gttccagagc cactgagtgg atcggcacat cgagtcgttc ctcgaaatgc tggctgccga
      601 gcggggcgcc gcacgcaata cgctgatggc atatcaggcg gaccttgagg atttcggcgc
      661 gttcgccgcg ggaaagggcc acctggtcgc cagctgcgac gctgcggtgc tgcaagccta
      721 catggccgga ctgcagcgag cggggctggc ggcgcgcacg gcggcgcggc ggctttcggc
      781 actgcggcag ttccatcgct tcctgctgcg cgagggtatc cgcacggacg atccgaccac
      841 gctgctcgat gcgccccgat tgccgaagac actgccgaaa tatctgacgg agcaggaagt
      901 cgatgcgctg cttggcgcct gcgaaccgat ggccggacag cgcggcttgg ttgcccgggc
      961 ggcgctggaa atcctgtatg cgacagggct gcgggtgtcg gagttgctgt cgctgccgcg
     1021 gcaggcgctg ggaggggacg ccaccgtgct gctgatccgg ggcaagggcg gcaaggaacg
     1081 catcgtcccg ttgtcagatc gtgcgaaaat cgcggctgag gccttggtgg cggcgcagaa
     1141 gaaggcgact cgctatctgt ttccgggtcg tgccgccaac cgtgcgctga cgcggcaggg
     1201 gtttttcctg ttgctcaaac aggtcgcgct cgaggcgggg atcgatcccg ggcgtatttc
     1261 gccgcacgtc ctgcggcatt cgtttgcgtc gcatctgctg gcgcgcggcg ccgatctgcg
     1321 cagcctgcaa ctgctgcttg gtcatgccga tatcgccacg acgcagatct atacgcatgt
     1381 attggcggag cggttgcaga ggctggtgga ggcgcaccat ccgctggctc acggtgagac
     1441 ggaatagagc ggcgctggcg aggaagggcg gcggcgatca ctccgccagg accacggctg
     1501 ggcggcgcgc gattcgcagg accatcaggg acgcgagcag gcacgcagcg ccgctggcga
     1561 tgaacgcggc gagatagctg ccggtggcat tgcgcgccaa gccgccgcca agtgcggcga
     1621 ttgcgccgcc catctggtgt ccgcagacaa tccacgacac gatcaccgga gagtccctct
     1681 tgccgaatac ctcggtcgtc aggccgacgg tcggcgggac cgtcgctacc cagtccaacc
     1741 cgtaaaacac ggcgaacacc gacaggctga gcatgtcgaa gttggtgaac ggcaggagca
     1801 cgagagaaag cccgcgcagg ccatagtacc aaaacaagag aacgcgggca ttgaaacggt
     1861 cggtgagcca gccggacagt gtggttccga tcaggtcaaa cacgcccatc gcggcgagga
     1921 ggctggctgc ctgcacctcg ggaatgccgt ggtccatgca gaaagggatc aggtgcgtcg
     1981 ccacaaggcc gttggttgag aagccgcaga cccagaaggt agcgaacagc agccagaagt
     2041 ctacggaacg cgcgccgcgt gccaggccgc tcaatgcgac gcgaaatgga ttgacagcgc
     2101 tcggtgccag tggcggttcg gcggctgcgc cgtatgggcc gagaccgaca ctgccgggac
     2161 tttcggctag catcagcagt accagcggga tcaggaccag cagcacggcg gcgaccgccc
     2221 agggaacgct ctgccagccc aggtgcgatg cgaggctggc gagcaacggc agaaagatga
     2281 gctgaccgct ggcgttgctc gctgtcagca aaccgacgac gaggccgcga tgagcgacga
     2341 accaacggtt cgccacggca gttgctaggc cgaccatgcc ggcgcccgag cctacgccca
     2401 ccagcacgcc acaagtggcg tagagttccc acggtgtatg ggcgaagctg ctgccggcgg
     2461 ccccgagcag gagcaggatc agagccagca ccacggtgc
//
LOCUS       DATGEM010000649         7177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1300071, whole genome shotgun sequence.
ACCESSION   DATGEM010000649 DATGEM010000000
VERSION     DATGEM010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300071"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VKQ27_20025"
     CDS             complement(<1..421)
                     /locus_tag="VKQ27_20025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91283.1"
                     /translation="MPDEGYQRITAQVHVAHPPTARRTLVLAACLMATFMAAVESSIV
                     ATAMPTIVADLGGFNLFSWVFAVYLLTQAVCIPIYGRLADVYGRKKVFYFGAGLFLIG
                     STLCGLSRNMLMLILFRALQGFGAGGVQPIATTILGDI"
     gene            547..2595
                     /locus_tag="VKQ27_20030"
     CDS             547..2595
                     /locus_tag="VKQ27_20030"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLY91284.1"
                     /translation="MAKSVDIGVDIGGTFTDIVCRRAGEPMRTLKIPTTRGDPSQAVL
                     NAVTHLARELDVPAEDIARFLHGTTIATNAVLERKGAKIGLIASEGFRDVLEIGYQLR
                     QDLHRILLEPVTPVFLAPGAQRKEVREQVSAQGEVIAPLDEASVREAVAELVADGVQA
                     IAICYLFSFLHPEHEQRTRALIEAAHPGIAVSLSSEVDPTFREYERTVVTAFDAYMKP
                     VVSRYLEHLDAGLRAAHVTAPLQIMQSRGGLAGTSVARKRPVRLFLSGPAAGVIGGAI
                     VGRSSGYRDLITIDVGGTSSDIALVEGGEPVIRAQGNIAGYPVRVPMVDVNAIGAGGG
                     SIAWIDGAGGLRVGPHSAGSEPGPACYGRGGELATVTDASVVLGWLDPAYFAGGSVAL
                     DPALARAAVERCVAQPLGMSVEDAALGIHRVVNAQMVEGMRLVSIRRGLDPRRFTLVA
                     LGGAGPIHATSLAAELGIAGVLIPRNPGVLSAAGLLAAPIEHEVAVAFPQPLASVDFA
                     DVRVVLDELDRSCSALMAEESVGDLPVSIQYSADVWYIGQSYHLEVPLDASAADPLWA
                     LYRDFLKLHDRIYGHATKQPAALVNLRAVHRAGGSDHLDEGTYRAQDIDPRKSDRPIR
                     VAGARAPVTAAIYHRAAMPAGTIFAGPAIVEQDDTTTLVEPGWHGVVLDNGNLLLTRG
                     "
     gene            2597..4273
                     /locus_tag="VKQ27_20035"
     CDS             2597..4273
                     /locus_tag="VKQ27_20035"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLY91285.1"
                     /translation="MDIDPITLEVLRNKLDGIANEMQLTLVRSAFSAIVKEGLDASAS
                     LFTLQGETLAQALAIPVHLGALTPMVGRLLKDVPLDRMGESDIWILNDTYLGGTHLPD
                     IAIMMPVFADGRVIAISAAMTHHQDVGGMAPGSTPTNATEIFQEGIRIPLLQFRRGEL
                     LNDTLIAMLRQNVRLPDVLMGDINAQVAACTIGARRLSELAGGFDRDDLAALFDELLN
                     RSETMTRQALRGLPEGTYRHVDYLDNDGIELDRLVRIEVAVTIADGTMTCDFTGTSPQ
                     VRGPFNCMASGPYAAACFALRSVTDPEGHIPNNGGCFRPIAFNLPEGTLVNPREPAPT
                     GCRTSTIKRITTCILGALRQAAPDRVPADSGSEIVIMHFGGQRSDGRRYVTSQLLVAG
                     SGASPQLDGVDVIETDASNCLNVPAEALMLEAPIRVHRIALARDSGGPGTHRGGLGGE
                     FEYELLDGEATVTYRGERHLCEAEGVADGMAGGRASAVIRRADGSEEIVKSKIVSTLR
                     KGDRFVIATAGGGGHGVPADRDPALLQADLRNGKVSLEAAREFYGYRGNV"
     gene            4266..5063
                     /locus_tag="VKQ27_20040"
     CDS             4266..5063
                     /locus_tag="VKQ27_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY91286.1"
                     /translation="MSEPRDLHDQALRDRAVRAARGQEPFDVLLTGGNIADVATGEFR
                     PADVGLVGPLIASVHPSGSRSDAHECVALDGQIIAPGLIDSHLHIESSMVTPRRYAEV
                     VVPQGTTTISWDPHEIGNVAGLDGLRWAIAATQGLPLRIVVLAPSCVPSAPGLERTGA
                     DFGPDEMREMLRWPAIGGVAEVMDMRGLLDRGARMSGIVDAGLASGKLVCGHARGLAG
                     RDLQGFAAAGIESDHEITSGDDLLAKLRAGFTVELRGSHDYVLPGAV"
     gene            5103..6038
                     /locus_tag="VKQ27_20045"
     CDS             5103..6038
                     /locus_tag="VKQ27_20045"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HLY91287.1"
                     /translation="MCTDDVFPDDLVTAGGMNDVLRRLIARGLEPIQALRCATLNAAM
                     RLARRDLGLVAPGRRADLIVLSDLRDVVVTRVYASGRLVAQDGRLVKPLPASPPGAPV
                     ETMHVRRLTDDNFRLRANAGSVRLRTVSKPRFTEWGEVTAAVRDGVAVLPDDAIMMAV
                     IHRHAKADAMPVLGILQGWSTWRGALATSIAHDSHNLVVFGRDPGDMAAAANAVIEAS
                     GGMAVAAGGVVTARLKLPVYGLLSDAPAMEVAAELSALRAAADAIVDWQPPLLTFKAI
                     VGASLACNPGPHVTDLGITDGSTGEVFANALIDTA"
     gene            6092..>7177
                     /locus_tag="VKQ27_20050"
     CDS             6092..>7177
                     /locus_tag="VKQ27_20050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLY91288.1"
                     /translation="MSIPTYDWIAYHARARGDVVAMEDLATGRTFSYAEFDDRVGRLA
                     SGMRARLGVARGDRVAVLAHNSTDLFEIQFACERLGAVFNPLNWRLTLPELTFIVDDS
                     QPVVLFHDPEFADTALELSAACGVRHLCGRDPHDSAYEQLIAAGAGDVAPEPLTHDDV
                     ATILYTSGTTGRPKGAMITYGMRFWQAINLTGPCRVTVDSTTLVVLPLFHVGGLDVFA
                     NPVFHFGGKAIVMRAYDPALALRLFTDCEVNVTHFVGAPAHFQFMAQLPQFADAVFNP
                     NLLAYAAAAPVPLPLLRQWQERGLSLLQLYGMTEACGVITSMEPWDAGRKAGSAGLPC
                     LHFDARLVRADGTDANVGEIGEIWVRGP"
BASE COUNT         1210 a         2004 c         2619 g         1344 t
ORIGIN      
        1 agatgtcgcc gaggattgtc gtggcaatcg gctgcacgcc acccgcgcca aagccctgca
       61 aggcgcggaa cagaatcagc atcagcatgt tgcgcgacag gccacacagc gtggagccga
      121 tcaggaacag gccagcacca aagtagaaga ccttcttgcg accataaaca tcggccagtc
      181 gcccgtagat cgggatgcag acggcctgcg tcagcagata gaccgcaaac acccaggaga
      241 acaggttgaa tccgcccagg tccgcaacga tcgtcggcat cgccgtggcg acgatggagc
      301 tttccactgc ggccatgaac gtcgccatca ggcatgcggc gagcaccaat gtgcggcggg
      361 ccgtgggcgg atgggcaacg tggacttggg cggtaatccg ctgatagccc tcgtcgggca
      421 caggctcgcc tcgttatggt tgggacgcgc ggaggttgtg tcactgcgaa ggcgcgctgg
      481 caacctgcac gatcgcaggc cagccgtgct atccaggcag acaattctgc ttgacgggac
      541 acttgcatgg cgaagtctgt cgacattggc gtcgatatcg gcggcacatt taccgacatc
      601 gtatgccgcc gcgccggcga gccgatgcgc acgctgaaaa tccccaccac gcggggcgat
      661 ccgagccagg cggtgctgaa cgcggtgacc catctcgcgc gcgagttgga cgtgcccgcc
      721 gaggacattg cacggttcct gcatggcacg acgatcgcca ccaatgcggt gctggagcgg
      781 aagggtgcga agatcgggct gattgccagc gaggggttcc gcgacgtgct ggagattggc
      841 taccagcttc gccaggacct gcaccgcatc ctgcttgagc cggtgacgcc ggtgttcctg
      901 gcgccgggcg cgcaacgcaa ggaggtgcgc gagcaggtct cggcccaggg cgaggtgatc
      961 gctccactcg acgaggcgtc ggtgcgcgag gcggtggctg aactcgtggc ggatggcgtg
     1021 caggcgatcg caatatgcta cctgttttcg ttcctgcacc cggaacacga acagcgtacg
     1081 cgcgcgctga tcgaagcggc gcatcccggt atcgcagtgt cgttgtccag cgaggtcgat
     1141 ccgacattcc gcgaatacga gcggacggtg gtcacggcgt ttgacgcgta tatgaaaccg
     1201 gtggtgagcc gctatcttga gcatctggat gcagggctgc gggcggcgca tgttacagcg
     1261 ccgcttcaga tcatgcagtc ccgcggcggg ctggccggca cctctgtggc gcgtaagcgg
     1321 ccggtgcggc ttttcctgtc gggcccggca gcgggtgtga tcggcggtgc gatcgtcggg
     1381 cgatcttcgg ggtatcgcga cctgatcacg atcgatgtgg gcggcacgtc gagcgacatc
     1441 gccctggtgg aaggcggcga gccggtgatc cgcgcgcagg ggaatatcgc gggctatcca
     1501 gtgcgtgtgc cgatggtgga cgtgaacgcg atcggtgccg gtggtgggtc gatcgcgtgg
     1561 attgatggtg ccggtggatt gcgcgtgggg ccgcattcgg cgggatcgga gccggggccg
     1621 gcatgttatg ggcgcggtgg cgagctggcg acagtgacgg atgcgtcggt cgtgctggga
     1681 tggctcgatc cggcgtattt cgcaggtgga tcggttgcgc tggatcctgc gttggcgcgc
     1741 gcagccgtgg aacggtgtgt ggcgcaacct ttggggatgt cagtagagga tgctgcgctc
     1801 ggaatccatc gggtggtgaa tgcgcagatg gtggagggga tgcggctggt gtcgatccgg
     1861 cgcgggttgg atccaaggcg gttcacgctg gtggcgttgg gtggtgcagg gccgatccat
     1921 gctacgtcgc tggcggcgga attggggatt gcgggtgtgc tgatcccgcg caatccgggc
     1981 gtgctgtccg cggcaggttt gctggcggcg ccgatcgagc atgaggtggc agttgctttt
     2041 ccgcagccgc tggcgagcgt ggattttgcc gatgtgcgcg tggtgctgga tgagctggat
     2101 cgatcgtgct cggcgctgat ggcggaggag agcgtcggcg atctgccggt ttccatccag
     2161 tattccgcgg atgtgtggta cattgggcag tcgtatcatc tggaagtgcc gctggatgcg
     2221 agcgcggcgg atccgctgtg ggcgttgtac cgcgatttcc tgaagctgca tgaccgtatc
     2281 tacggacatg cgacgaagca gcctgccgcg ctggtcaacc tgcgggcggt gcatcgggca
     2341 ggtggcagcg accatctgga tgaaggcacc tatcgcgcgc aggacatcga tccccgcaag
     2401 tcggatcggc cgatccgcgt ggcgggcgcg cgtgcgccgg tgacggcagc gatctatcat
     2461 cgggcggcga tgcccgccgg cacgatcttc gccgggccgg cgatcgtgga acaggatgat
     2521 accaccacgc tggtcgagcc aggctggcac ggcgtggtgc tggacaacgg caatttgctt
     2581 ctgacgcgag gctgacatgg acatcgatcc catcacactg gaagtactgc gcaacaagct
     2641 cgacggcatc gcgaacgaga tgcagttgac gttggtccgc agtgcattct cggcgatcgt
     2701 gaaggaaggg ctggatgcct cggcgagtct gttcacgctg caaggcgaga cgctggcgca
     2761 ggcgcttgcc atcccggtgc atctgggggc gctgacgccg atggtcggac ggctgctgaa
     2821 ggatgtgccg ctcgaccgga tgggcgaaag cgacatatgg atcctgaacg acacgtatct
     2881 gggcggtacg catctgccgg atatcgcgat catgatgccg gtgttcgcgg atgggcgggt
     2941 gatcgcgatc agtgccgcga tgacgcatca ccaggatgtc ggaggtatgg cgcctggctc
     3001 caccccgacc aatgcgacgg agatcttcca ggaaggcatc cgcatcccgt tgctgcagtt
     3061 ccggcgcggc gagctactga acgacacgct gatcgcgatg ctgcgacaga acgtgcggct
     3121 gcctgatgtg ctgatgggcg atatcaacgc acaggttgcc gcttgcacga tcggcgctcg
     3181 gcggttgagc gagctggcag gagggtttga ccgtgatgat ctggcggcgc tgttcgacga
     3241 attgctgaac cggtcggaga cgatgacgcg ccaggcactg cgcgggctgc cggaagggac
     3301 ttaccggcat gtcgattatc tcgacaatga tgggattgaa ctcgaccggc tggtgcgcat
     3361 tgaagttgcg gtgacgatcg ccgacggcac gatgacgtgc gatttcaccg gcacatcgcc
     3421 gcaggtgcgg ggccccttca actgcatggc ctcggggcct tatgcggcgg cgtgcttcgc
     3481 gttgcgctcg gtgacagatc cggaggggca cattcccaac aatggcggat gcttccggcc
     3541 catcgcgttc aatctgccgg aaggaacgct ggtgaacccg cgtgagccgg cgccgacggg
     3601 gtgccgcacg tcgacgatca agcggatcac cacgtgtata ctgggagcac tacggcaggc
     3661 tgcaccggac cgggtaccgg ccgattccgg cagcgagatc gtcatcatgc atttcggcgg
     3721 gcagcggtcc gatggcaggc ggtatgtcac gtcacagttg ttggtggcag ggagcggcgc
     3781 ttcaccgcag ctcgatgggg tcgatgtgat cgagacggat gccagcaatt gcctgaacgt
     3841 gccagccgag gctctgatgc tggaggcgcc gatccgcgtg caccgaattg ctctggcgcg
     3901 ggattcaggt gggccaggaa cgcatcgcgg cggactcggt ggcgaattcg agtacgaact
     3961 gctggatggc gaggcgacgg tgacgtatcg cggtgaacgc catctctgtg aagcggaggg
     4021 tgtggcggat ggtatggctg gtggtcgcgc gagcgcagtg atccggcgtg ccgatgggag
     4081 cgaagagatt gtgaaatcga agatcgtatc taccctgcgc aagggtgacc gcttcgtgat
     4141 cgcgacggcg ggtggtggcg gacacggcgt gccagcggac cgcgatcctg cattgctgca
     4201 agccgatctg cgcaacggga aggtgagttt ggaagcagca cgagagttct acggatatcg
     4261 cggcaatgtc tgagcctcgg gatctgcatg atcaggcgct gcgcgatcgc gcggtgcgtg
     4321 cggcgcgtgg tcaggaaccg ttcgacgtac tgcttaccgg gggcaacatt gctgatgtcg
     4381 cgactgggga gttccgtccc gcggatgtcg ggctggtggg gccgctgatt gccagcgtgc
     4441 atccgagtgg ttcgcgcagc gatgcgcatg agtgtgttgc gctggacggc cagatcatcg
     4501 cgccaggact gatcgattcg catctgcata tcgagagctc gatggtgacg ccgcggcgct
     4561 atgccgaggt cgtggtgcca cagggcacaa caacgatatc ctgggacccg cacgagatcg
     4621 gcaatgtggc ggggctggat ggcctgcgct gggcgatcgc ggcgacgcag ggtttgccgc
     4681 tgcgcatcgt ggtgctggcg ccgtcgtgtg tgccctcggc gcctggcctg gagcgcacgg
     4741 gggcagattt cggcccggac gaaatgcgcg agatgctgcg gtggccggcg atcggcggcg
     4801 ttgccgaagt gatggacatg cgcggcctgc tggaccgcgg cgcgcgcatg agcggcattg
     4861 ttgatgccgg gctggcgagc ggcaagctcg tgtgtggcca tgcgcgcggg ctggcgggac
     4921 gcgatctgca ggggttcgcc gcggctggta tcgaaagcga tcatgagatc accagtggcg
     4981 atgatctgct ggccaaattg cgcgcggggt tcaccgtcga gttgcgcggg tcgcatgatt
     5041 acgttctgcc tggcgcggtc taggctctga gcaccctgcc ccacatcccg cagacactga
     5101 cgatatgcac cgatgatgtt ttcccggacg atctggtgac cgcgggcggc atgaatgacg
     5161 tgctgcgccg gctgatcgcg cgcggcctgg aaccgatcca ggcattgcgg tgcgcgacgc
     5221 tgaacgcggc gatgcggttg gcacggcgcg atctggggtt ggtggcgcct gggcgtcgcg
     5281 ccgacctgat cgtgttgtcg gatctgcggg atgtcgtggt gacgcgtgtc tatgcgtcgg
     5341 gacgattggt ggcccaggat gggcggctgg tgaaacctct tccagcgagc ccgccggggg
     5401 caccggttga gacgatgcat gtgcggcgtc tgacggatga taacttccgg ttgcgggcca
     5461 atgcgggctc agtgcgattg cgcacggtct ccaaaccacg ttttaccgag tggggcgagg
     5521 tgacggcagc agtgcgcgat ggcgtggcgg tgttgccaga cgacgcgatc atgatggcgg
     5581 tgatccatcg ccacgcgaaa gctgacgcaa tgccggtgct gggcattctg caaggctgga
     5641 gcacgtggcg cggcgcgctg gcgacatcga tagcacatga cagccacaac ctcgtggtgt
     5701 tcgggcgtga tccgggggac atggcggcgg cggccaatgc agtgatcgag gcaagtggcg
     5761 gcatggctgt cgccgcgggc ggtgtggtga cggcgcgtct gaaattgccg gtgtacggat
     5821 tgctgtcgga cgcgccggcg atggaagtcg cagcggaact gagcgctctg cgcgcggcgg
     5881 cggatgcgat cgtggattgg cagccgccgt tgctgacatt caaggcgata gtcggcgcca
     5941 gcctggcctg caatcccgga ccgcatgtga cggatctcgg catcaccgac ggcagcacgg
     6001 gtgaggtgtt cgcgaatgca ctgatcgaca ccgcttagcg gcggaataca tcaagccggc
     6061 gacagaccgg cccaacaggg aggaagcgac catgtcgata ccgacgtacg actggattgc
     6121 ctatcacgca agggcgcgtg gcgacgtcgt cgccatggag gacctcgcga ccggccgtac
     6181 tttttcctac gcggagttcg atgatcgcgt tggccggctt gcgtccggga tgcgagcgcg
     6241 gctcggcgtt gcgcgcggcg atcgggtcgc ggtgctggca cataattcga ccgatctgtt
     6301 cgagatacag ttcgcatgcg agcggcttgg cgctgtgttc aatccactga actggcgcct
     6361 gactctgccg gagctgacat tcatcgttga cgattcgcag ccagttgtac tgttccacga
     6421 tccggagttc gcggacaccg cgctggagtt gtccgcagca tgcggggtgc ggcacctgtg
     6481 cggacgtgat ccgcacgaca gcgcgtatga gcagctaatt gccgctggtg cgggcgatgt
     6541 ggcgccggaa ccgctgacgc acgatgatgt agcaacaata ttgtatacgt caggtacgac
     6601 cggacggccg aaaggggcaa tgatcaccta tggcatgcgc ttctggcagg ccatcaacct
     6661 gactggcccg tgccgtgtga cagtggacag cacgactctt gtggtgctgc cgttgtttca
     6721 tgttggcggc ctggatgtgt tcgccaatcc ggtgttccat tttggcggca aggcgatcgt
     6781 gatgcgggcc tatgatcctg cactggcatt gcggctgttc acggactgtg aggtcaacgt
     6841 cacgcatttc gtcggcgccc cggcgcattt ccagttcatg gcacagttgc cacagttcgc
     6901 cgacgcggta ttcaatccga acctgctcgc ctatgccgcc gccgcgccgg taccgctgcc
     6961 tctactgcgc caatggcagg agcgcgggct atcactgctg cagctttacg gcatgaccga
     7021 ggcgtgcggc gtgatcacct cgatggaacc gtgggatgcg gggcgcaagg ctggatctgc
     7081 cggattgccg tgcctgcatt tcgatgcacg gctggtgcgg gcggatggca ctgatgccaa
     7141 tgtcggggag attggcgaga tctgggtgcg agggccc
//
LOCUS       DATGEM010000650         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1300180, whole genome shotgun sequence.
ACCESSION   DATGEM010000650 DATGEM010000000
VERSION     DATGEM010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300180"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..609)
                     /gene="coaD"
                     /locus_tag="VKQ27_20055"
     CDS             complement(94..609)
                     /gene="coaD"
                     /locus_tag="VKQ27_20055"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLY91289.1"
                     /translation="MAQPVKKLRVGLYPGTFDPVTNGHLDVIARAARLLDKLVVGVAI
                     NTGKGPLFTLEERVEMVQAEIARIATENGMVIEVRPFDTLLIDFARRVGASMIVRGLR
                     AVSDYDYEIQMAGMNYRMAPDIETVFLMASERHQFIASRLVKEVALLGGDISSFVPPL
                     TLERMLQRIRG"
     gene            complement(611..>2789)
                     /locus_tag="VKQ27_20060"
     CDS             complement(611..>2789)
                     /locus_tag="VKQ27_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HLY91290.1"
                     /translation="NIPPHNPGEVIDATLALIAEPDLSLEDLMRIVPGPDFPTGGFIV
                     GRSGIRAAYETGRESITIRARHEFEDMARDREAIIFTEIPYQVNKKTLLERIGELVRE
                     KQIEGISGVRDESDRSGMRVVIELRRDATPEVVLNQLYRFTQLQITFGINMLALDKGQ
                     PRQLGLRDALLCFIRFREEVIERRARFELEKARKTAHNLVGLAIAVADIDEVIRLIRG
                     SPDAAAARAALMGRDWPAADIAPLLALIDEPGNVISENNTVRLTEEQARGILEIRLQR
                     LTGLEREKIHQDLEKIGAEIRELLAILSSHPRRMEVMREELLKAREALATPRMTDIID
                     ALADQEDESLIEPGQMVVTITRDGFIKRTTLETFRAQNRGGRGRSGASTRGDDIVTRS
                     FHAHTHQWVLFFSLGGKVFREKVWRLPEAGPTAKGRALVNLLPELGGDTITTVLPLPQ
                     DETLWDSLHLLFATASGSVRRNRLSDFRNVRSVGLIAMKLDEGDRLIGVATCREGQDA
                     FLATRRGRCIRFELSDETVRVFAGRDSSGVRGIRLADGDEVISLSILQHVRATNEERE
                     AYVKQANARRRSNGEDEVDASAEAEEPVTDAQLDEARIAQLAQSEEFLLTVTNAGFGK
                     RTSAYEYRVTGRGGQGIANTTLAPRNGTAVVATLPVVDEDDVMLVTDAGRLIRVPADQ
                     VRITGRQAMGVTLFRVDADEHVTSVFPVMEDTVEDSGGSEDEE"
BASE COUNT          499 a          914 c          845 g          531 t
ORIGIN      
        1 gtggcgaccg cggcgcccgc gatcaggctg cgtcgaaaca ttggcgctct cctctcagca
       61 aacggctggc atgtcccggg cctgacccgg tgatcagccg cggatacgct gcaacatacg
      121 ctccagcgtc agcggcggaa cgaaggacga aatatcaccg cccagcagcg ccacttcctt
      181 aacaagccgc gaggcgatga attggtggcg ctcgcttgcc atcaggaaca ccgtctcgat
      241 gtccggcgcc atccgatagt tcatgccagc catctgaatc tcgtagtcgt agtccgagac
      301 cgcacgcaaa ccccgtacaa tcatcgaggc gccaactctc cgtgcgaaat cgatcaacaa
      361 tgtgtcaaac ggccgcacct cgatcaccat gccgttctcg gtggcgatac gggcaatctc
      421 tgcctgcacc atctcgacgc gctcttccag tgtgaacagc ggccctttgc cagtgttaat
      481 ggcaacgccg acaaccaatt tatccagcag gcgggcagcg cgggcaatca cgtcgagatg
      541 gccgttggtc accggatcga acgtgccggg atacagaccg acacgaagct tcttcacagg
      601 ctgcgccatc ctactcttcg tcctccgaac cgccgctgtc ctcgacggta tcttccatca
      661 ccgggaacac gctggtgaca tgctcgtcgg cgtcaaccct gaacagcgtc acacccatgg
      721 cctgacgccc ggttatacgc acctggtcgg cgggaacccg gatgagccgg cccgcatcgg
      781 tcaccagcat cacgtcgtcc tcatccacca ccggcagcgt cgccacaacc gcggtgccat
      841 tacgcggtgc cagtgtggta ttggcaatcc cttgccctcc ccggccagtc acgcgatatt
      901 cgtaggccga ggttcgcttg ccgaagccgg cattggtgac agtgagcagg aattcttcgc
      961 tctgcgccag ttgagcgatc cgtgcctcat ccaattgcgc atcggtgacg ggctcttcgg
     1021 cttctgccga tgcgtccacc tcgtcctcac cattgctccg gcgccgggca ttggcctgct
     1081 ttacataggc ctcgcgctcc tcgttggttg cccgaacatg ctggaggatc gacaggctga
     1141 tgacttcatc gccgtcagcg agcctgatgc cgcgcacgcc agagctgtca cggccggcaa
     1201 atacccttac cgtctcatcg gacagctcaa agcgaatgca gcgacccctg cgcgtggcca
     1261 ggaaggcatc ctgcccctca cggcaagtcg caacgccgat gagccgatcg ccttcatcca
     1321 atttcattgc gatcagaccc acggaacgca cgttgcggaa atcggagagc cggttgcggc
     1381 gcacgctgcc ggatgccgtc gcgaacagca gatgcaagct gtcccacagt gtttcgtcct
     1441 gcggcaacgg tagaaccgtg gtgatcgtgt cgccgccaag ctccggtagc aggttgacga
     1501 gcgccctgcc ctttgcggtg ggtcctgctt caggcagccg ccagaccttc tcacggaaga
     1561 ccttgccgcc gagcgagaag aacagcaccc actgatgggt gtgggcgtgg aagctgcggg
     1621 tgacgatgtc gtcgccgcgg gtggatgcgc cgctgcgacc acgaccgcca cggttctgcg
     1681 cgcggaaagt ttccagcgtt gtgcgcttga tgaagccgtc gcgcgtgatg gtgaccacca
     1741 tctggccggg ctcgatcagg ctttcgtctt cctgatcggc gagcgcgtcg atgatgtctg
     1801 tcatgcgcgg cgtcgcgagc gcctcgcgtg ccttcagcag ctcctcgcgc atcacttcca
     1861 tgcggcgcgg atgcgaactc aggatggcca gcaactcgcg gatttcggcg ccgatcttct
     1921 ccagatcctg gtggatcttc tcgcgctcca ggccagtcag gcgctgcagg cggatttcca
     1981 ggatgccacg ggcctgttct tcggtcaggc gcaccgtgtt gttctcgctg atcacgttgc
     2041 ccggttcgtc aatcagcgcg aggagcggcg cgatatcggc ggccggccaa tcgcggccca
     2101 tcaaggcggc gcgcgcggcg gctgcgtcgg ggctgccgcg gatcaggcgt atcacctcat
     2161 caatatcggc gacggcgatg gcgagaccaa cgagattgtg ggcggtcttc cgcgccttct
     2221 ccagttcgaa gcgcgcgcgc cgctcgatca cctcctcgcg aaagcggatg aagcacagca
     2281 gtgcgtcgcg caggccaagc tggcgaggct ggcctttgtc cagcgccagc atgttgatgc
     2341 cgaatgtgat ctgcagctgc gtgaagcgat aaagctgatt cagcaccacc tcgggcgtcg
     2401 catcgcgccg cagctcgatg accacgcgca tgcctgagcg gtcgctttcg tcgcgcacgc
     2461 cgctgatgcc ttcgatctgc ttttcgcgca ccagctcgcc gatgcgttcg agcagcgtct
     2521 tcttgttcac ctgatacggg atctcggtga agatgatggc ttcgcggtca cgcgccatgt
     2581 cctcgaattc gtggcgggcg cggatcgtga tggactcgcg gccggtctcg taggcagcgc
     2641 ggatgccgga acggccgacg ataaagccgc cggtcggaaa atccggacca ggcacgatgc
     2701 gcatcagatc ttcgagcgaa agatcgggtt cggcgatcag cgccagggtc gcatcgatga
     2761 cctcgccagg attgtgcggc gggatattg
//
LOCUS       DATGEM010000651         9046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1645537, whole genome shotgun sequence.
ACCESSION   DATGEM010000651 DATGEM010000000
VERSION     DATGEM010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645537"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VKQ27_20065"
     CDS             <1..512
                     /locus_tag="VKQ27_20065"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HLY91291.1"
                     /translation="TTAPTSFHLQNWRFIAVRTPEAKARLRPIAWDQPATTDAAVTFM
                     VCGQLADSSVIPERLAPLVEAGIMPATMVPDWEIPARDLYMKYPQRQRDEAVRTGSFG
                     AAAMIYAARSLGLGSTPMIGFDAEAVQREFGLAKDEVPVMLLSVGPVRPGNWPQKPRR
                     PVDDVLDLV"
     gene            807..1625
                     /locus_tag="VKQ27_20070"
     CDS             807..1625
                     /locus_tag="VKQ27_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91292.1"
                     /translation="MEHVAPTARTEMPPDPPKDPPATTLPGNIAGLLHVARYLLGYGR
                     HLADTIRQRAAAPSFDAIAACFGTSDLTTIYAHLHRGILRCIALERVLLARAATGKDV
                     TFIERNTSIALLPEPEPAQAEAQQQPAATTPLLPARRRATRASRPAGWDDPELFMPTL
                     EDLERQIRRQPIGRVFSDICLDLAVVPGFCHPAFWNALFEIMTYFGSSVNKVMQEKTR
                     RRAAFGKEQDRKRGANWDWVNIPRDKLRDVLGFFIGEPPINPFDPAAAIATAPP"
     gene            complement(1579..1842)
                     /locus_tag="VKQ27_20075"
     CDS             complement(1579..1842)
                     /locus_tag="VKQ27_20075"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91293.1"
                     /translation="MPIVASSVGGPSEILEHERTALLVPPRDPVALARSFLGLIDAPR
                     LRSEPGLAAARAVRQEWLYARVVQKGWRVRVGRWRWRRRGRMG"
     gene            complement(2005..4452)
                     /locus_tag="VKQ27_20080"
     CDS             complement(2005..4452)
                     /locus_tag="VKQ27_20080"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HLY91294.1"
                     /translation="MPTSSTVQNTDFTRDVLGRYVCNGLDEALASAVAHPFEVIVVGG
                     GSFGPIFAQHFMYRDATRARRILILEAGRFTLPEHVQNMPMLGLNVPGPTTVDPGALR
                     NEVWGLPWRTDVPQGFPGLAYAIGGRSLFFGGWSPRLLPSELPAAWPAAVVNDLNGPL
                     ADGRPGYFGQAAEQIGTDVTNDFISGPLHEALRAQLKQAIDAGNVTDAIPLAQLPLTL
                     GGIPDAQADLFKLEAPLAVQARPPRSGFFPFNKFSSAALAIEVARAAQGESGGDDTRK
                     RLMLVPDCHVVRLVTDGAGPIRRVVAIETSRGTVPVPEEAVVVLALGTIENARLAALS
                     LPGLPDGQRIGANLMVHLRSNLTIRIPRASVAGLDPNIGELQASALFVKGRHDHGDHQ
                     FGHFHLQITVAGLATPGTDSEAELFKKIPDIDTIDRFRGMNDQQIVITVRGIGESRPG
                     NAASLVTLAGETDEFNVPRGFVSINPSAEDRLLWEAMDKAAEDVALAFANGNAYEVLV
                     GGNFQPVAAGQSALTVISRNNRRDGLGTTHHEAGTLAFGDNPATSVTNPDARFHHVPN
                     LYVAGPALFPTVGSPNPMGTGTALARRLGDHLASPFIADPGFTSLFDGASLQHWRMST
                     IRNQPGRDDPGHFLVTNGALVTATGSDIGLLWHDQPTPPDFVLKLEWRRWQEDANSGV
                     FLRFPNPETRNYDNTAFVGVDFGFEVQIDQLAAPDGSPLHKTAAIYGFRGPANPNALP
                     VRPVGEWNAFEIRVQGQNYRVLLNGVEVTVFNFVPGSDAAHPDRGLPSSNAVPRFIGL
                     QTHTGKVAFRRLQIQPL"
     gene            complement(4722..6314)
                     /locus_tag="VKQ27_20085"
     CDS             complement(4722..6314)
                     /locus_tag="VKQ27_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91295.1"
                     /translation="METDAGRGGDVAHQYTRLEERILARDQVGAGEAFYDLVRAARPL
                     NEIVAECVRIHAPFTHVPYHERIDDGYPNFVNNDHCLLSARATLHLARLMPDGLAMLP
                     MAQTIWYIPTGLDIWNQKINKATGHYTRGFDGKIENPPAPVVYWPDQQPLRSREPLKE
                     RLGEWLTLVQRGEVLPAYRTFLGLMEEVADRKEVLAELVFAGMIDVQDRMYRNRSYTT
                     GHKAYRARSTVELGNALGWDNAHNVIYAGALDMAVGPRWHSLYEMACNTIKEEIEGVV
                     LHAVPYGGVSDAELAMLRNTGALQAEEEFEVMRGLLHEHSPASLDGVTRMLRAGKDPR
                     RIVDVIQIAAAEIVLATHGVNNFSMPMHCYEYTNALGWFYDTFEHPRRLRLLYVAAEF
                     VNMTAWHQKLSNACASSTIHAPASAQGLDRDEMLRRIDEAICSFDGPASLSWVQAYCS
                     SVPERVPLVRTLALAACKLGNDPHNQEIPQNMLEDFGKNQSARRDRLLLACAQHCASH
                     RKYGDPFEAAQRFARTMGVALG"
     gene            complement(6423..6812)
                     /locus_tag="VKQ27_20090"
     CDS             complement(6423..6812)
                     /locus_tag="VKQ27_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLY91296.1"
                     /translation="MSSILSHITVGVRDLARATAFYDAVLGELGITRGKSGEGFAGWG
                     SGHEGGFFVMLPINGQPASVGNGVSVSFLAPSRGAVDRFHAKALGLGATDEGKPGLRP
                     NYHADYYAAYVRDPDGNKVAAVCHAAV"
     gene            complement(7064..7813)
                     /locus_tag="VKQ27_20095"
     CDS             complement(7064..7813)
                     /locus_tag="VKQ27_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLY91297.1"
                     /translation="MIDLTRAPASLPPGRRVYAIGDIHGCDIQLGNLHEMIAEDLARR
                     TVDEPVLLHIGDYVDRGADTAGVLRRLLRDPVVSGMQVVNLVGNHDETMLHALSGDRP
                     AATDWLFAGGRPALESYGIDPDSPRESWPESVPVEHLEFLRNLTLTHREGGYFFVHGG
                     VRPGVPLDRQAREDLLRMRQPFLYSEMDFGAVVVHGHSPVKEPVVRHNRVAIDTGAVF
                     GGKMTCLVLEGETLGFLTADPVGEPVSIAHR"
     gene            complement(7825..>9046)
                     /locus_tag="VKQ27_20100"
     CDS             complement(7825..>9046)
                     /locus_tag="VKQ27_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HLY91298.1"
                     /translation="WFMRMPWKPGHSPLRHHPDYEAYLFGQWTHGAFDEFWQQLGIWT
                     AGWHERYSRAACVHMSSWWDPYPLTATGNYLGLKRAGRGQQRLILGPWTHGDRSNTSF
                     GDVEFGPNAPLDAWAGDWRAYRLRFFDHIVKGAALDEPAVRLFVMGGGSGRRNAAGKL
                     EHGGRWISAPDWPPPGVAFEAFHLHGDGGLDRVMPAVGAAPLSYDFDPSRPVPTIGGA
                     MSSLDPVAYAGAWDQVEGPDFLGCSPPYLPLASRRDVLVFQTPPLKSAVQVVGPIEAE
                     LFVSTDGPDTDFTAKLIDVHPASADYPKGYAMILTDGILRLRYAEDPTRPRLREPGEV
                     VKIRITLFPTANLFLPGHRIRLDVSSSNFPKFDVNPNTGEPEGRALRKRVAVNTVFVD
                     AARPSRVVLPLLPV"
BASE COUNT         1659 a         3235 c         2633 g         1519 t
ORIGIN      
        1 gcaccaccgc gccgacatcc ttccacttgc agaactggcg cttcatcgcc gtacgcacgc
       61 ctgaagccaa agcacggcta cgtccgatcg cctgggatca gcccgcgacc accgacgcag
      121 ccgtcacctt catggtctgc ggccagttgg ccgattcaag cgtaatacca gagcgcctgg
      181 caccactggt ggaagcgggc atcatgccgg caacgatggt gccggattgg gaaatccccg
      241 cccgcgatct gtatatgaag tacccgcagc gccaacgcga cgaggcagtt cgcacgggca
      301 gcttcggcgc ggcggcgatg atctacgcag cccgctcgct gggcctgggt tcgacgccga
      361 tgatcggttt cgatgccgaa gcggtgcagc gcgagttcgg actggccaag gatgaagtac
      421 cggtcatgct gttgtctgta gggccggtgc gtccgggaaa ctggccacag aagccgcgcc
      481 gtcccgtcga cgatgtactg gatctggtat agatttggtc aaatctctat ttgcggatat
      541 tacgatggcc agactgtatc ggcaaagccg aaccaggtgc cggcctgttc gaaaccggcc
      601 ctcgatacgc ccaccaggaa catgggttca ccccaaatat gttggcgccc ttcgcgcaga
      661 gcccgattgc gttcaacgat caccgcccgt tccgccccca gctgtatagt ccccacggtg
      721 tgcgctccag cgacgccaca gcataaattc ccatatccag aaaatattcc ttgcctatcc
      781 gcgccctccg ccctaagacc gccccaatgg agcacgtcgc ccccaccgcc cggaccgaaa
      841 tgccgcctga tcccccgaag gatccccccg ccaccactct gcccggcaac attgccggcc
      901 tcctccacgt cgcccgttat ctgctcggct acggccgcca cctggccgac accatccgcc
      961 agcgcgccgc cgctcccagc ttcgacgcca tcgccgcctg tttcggcacc tccgacctca
     1021 ccaccattta cgcccacctg caccgaggca tcctgcgctg catcgcgctg gagcgtgtcc
     1081 tccttgcccg cgccgccacc ggcaaggatg tcaccttcat cgagcgcaac acctccatcg
     1141 cgctgctgcc cgagccagag cccgcacagg ccgaagcaca gcagcagccg gccgccacca
     1201 cccccctcct gcccgcccgc aggcgtgcca cccgcgcatc acgccccgcc ggctgggatg
     1261 accccgaact cttcatgccc acactggagg atctcgagcg tcagatacgc cgccagccca
     1321 tcggccgcgt cttctccgac atctgcctcg atctcgccgt cgtcccaggc ttctgccacc
     1381 ccgccttctg gaacgcgctg ttcgagatca tgacttattt cggcagcagt gtgaacaagg
     1441 tgatgcagga gaaaacccgc cgccgcgctg cgttcggcaa agagcaggac cgcaagcgcg
     1501 gcgccaactg ggactgggtg aacatacccc gcgacaagct ccgcgacgtg ctgggcttct
     1561 tcatcggcga gccgccgatc aacccattcg accccgccgc cgccatcgcc accgccccac
     1621 cctgacccgc catcccttct gcacgacgcg tgcatacagc cattcttggc ggacagcgcg
     1681 cgcagcggcc aaacctggct cgcttcgcag tcgcggggcg tcaatcagcc ccagaaagct
     1741 ccgggccaga gccacgggat cacgaggagg taccagcaac gcggtccgtt cgtgttcaag
     1801 gatctctgag ggccctccta cacttgaggc aacgatcggc agctcatact cggcgcgatc
     1861 gagcgaatgg accgtgtata ctttgccgcc ccgggtgcga gcacgtacct atctggccaa
     1921 cggccaaagc cagaatacgt gaggtcgtaa ggtgcaggac gcgcatctcg gatcacccac
     1981 acggtccgcc tcagccaccc gcactcagag cggctggatc tgcaacctgc gaaacgccac
     2041 cttgccggta tgggtctgca agccgatgaa tcgcggaacc gcgttgctgc tcggcagtcc
     2101 ccgatcagga tgcgccgcat cggaaccggg cacgaagttg aagacagtga cctccacacc
     2161 attgagcagc actcgatagt tctggccctg caccctgatc tcgaacgcat tccattcgcc
     2221 caccggccgc accggcagcg cattggggtt cgccggcccc cgaaaaccgt agatcgctgc
     2281 ggtcttatgc agcggcgaac catcaggtgc cgcaagctga tcgatctgca cctcgaagcc
     2341 gaaatcgacg ccgacaaacg ccgtgttgtc gtagtttcgc gtctcggggt tcgggaagcg
     2401 caggaacacc cctgagttcg catcttcctg ccaacggcgc cactcgagct tcaggacgaa
     2461 atccggcggc gtcggctggt catgccacag caggccgata tcgctgcccg tcgccgtgac
     2521 aagcgcacca ttggtcacca ggaaatgccc cgggtcatcg cgaccgggct gattgcggat
     2581 ggtcgacatc cgccagtgtt gcagcgacgc gccatcgaac agtgacgtga agccggggtc
     2641 ggcaatgaag gggctggcaa gatgatcccc cagccggcgc gccagcgcgg ttccggttcc
     2701 catcgggttc ggcgacccga ccgtcggaaa caacgcgggg ccagccacat aaaggttcgg
     2761 gacatgatgg aaacgcgcgt ccggattggt cactgacgtc gcggggttgt cgccgaacgc
     2821 gagggttccc gcttcatgat gcgtcgtccc caggccgtcg cggcggttat tccgcgagat
     2881 gacggtaagc gccgattgac cggctgccac cggttggaaa ttccccccca ccagcacctc
     2941 gtatgcattg ccgttggcga acgccagcgc gacgtcctcg gccgccttgt ccatcgcttc
     3001 ccacagcagg cggtcttccg ccgacggatt gatcgatacg aaaccgcgcg gaacgttgaa
     3061 ctcgtctgtc tcgcccgcca gcgtgacaag gcttgctgcg ttgccgggtc ggctctcgcc
     3121 gatcccgcgg acggtgatga cgatctgctg gtcgttcatg ccgcggaacc tgtcgatcgt
     3181 gtcgatgtcc gggatcttct tgaacagctc cgcctcggag tccgtacccg gcgtggccag
     3241 gccggcgact gttatctgca aatggaagtg gccgaactgg tggtcgccgt gatcgtgacg
     3301 ccctttgacg aacaatgcgg atgcctgcag ttcgccgatg ttcggatcaa gtccggcgac
     3361 cgaggcacgt ggaatccgta tcgtcaggtt ggaacgcaga tgcaccatca gattggcacc
     3421 gatgcgctgc ccatcaggca atccgggcag cgacagggct gcaagccgcg cgttctcgat
     3481 cgtgccgagt gccaggacaa cgaccgcttc ctcgggcacc ggaaccgtgc cgcggctggt
     3541 ttcgatcgca acgacacgac ggatcgggcc agcgccgtca gtgaccaggc ggacgacgtg
     3601 acaatcgggg acgagcatca ggcgcttgcg ggtatcatcg cctcccgact cgccttgggc
     3661 agcacgggcg acctctattg ccagcgcagc gctggagaat ttgttgaacg ggaagaagcc
     3721 agatctcggc ggcctggcct ggacggcaag cggcgcctcc agcttgaaca gatccgcctg
     3781 cgcatccggt atcccgccga gtgtcagcgg caattgagcg agagggatcg cgtccgtcac
     3841 gttgcccgcg tcgatcgcct gcttgagctg ggctcgcaaa gcctcgtgca gcggacccga
     3901 gatgaagtca ttggtcacgt cagtgccaat ctgctcggcg gcctgcccga aatagcccgg
     3961 cctgccatct gccagcggac cgttgagatc gttgaccacc gccgcgggcc aggcagcggg
     4021 aagctcgctg ggcagcagtc gcggcgacca gccgccgaag aacagcgagc gcccgcctat
     4081 cgcataggcc aggcccggaa acccttgcgg cacatcggta cgccacggca gcccccagac
     4141 ctcgttgcgg agggcgcccg ggtcaaccgt ggtcggcccg ggaacgttca gcccaagcat
     4201 cggcatgttc tgcacgtgct cgggcagggt gaacctgcct gcctcgagga taaggatgcg
     4261 ccgggcccgg gtggcgtccc ggtacatgaa gtgctgcgcg aagatcggtc cgaatgaacc
     4321 gccgccaacg acgatgacct cgaacgggtg cgcaacggca ctcgccagcg cctcatcaag
     4381 accgttgcag acgtagcggc caagcacgtc acgtgtgaaa tcggtattct gtaccgttga
     4441 gctggtcggc atgagcgtct ccctcgcgaa gctgccttta ggctgtcttg gttttgcgat
     4501 cttggggtcg gtgacaggca cgtcgttgcc tgcattgttt cgttacatag cggtcggcga
     4561 cacacagttg gtgtgaccag gctcacagcc cgcacggcct tcccccgtca tggccggccg
     4621 ccgagccggc catcaggtgg cgcggtacca cgctgcgatt atcggctcac cgcagcatca
     4681 gcccccgcac agcgaacaac cccagccgca ggcccgcgcc atcagcccag cgccacgccc
     4741 atcgtcctgg caaaccgttg cgccgcctcg aacggatcgc cgtacttccg atgcgaggcg
     4801 caatgctgcg cgcacgccag cagcagccga tcccgccgcg cgctctggtt cttgccgaaa
     4861 tcctcgagca tgttctgcgg tatctcctgg ttgtgcggat cgttgcccag cttgcacgcc
     4921 gccagcgcca gcgtccgcac cagcggcacc cgctccggca cgctgctgca ataagcctgc
     4981 acccacgaca gacttgccgg accatcgaac gagcagatcg cctcatcgat ccgccgcagc
     5041 atctcatcgc gatccagtcc ctgcgcactg gccggcgcat ggatcgtcga cgacgcacac
     5101 gcattgctca gcttctggtg ccacgccgtc atgttgacga actcagccgc cacatacagc
     5161 agccgcagcc gccgaggatg ttcgaacgtg tcatagaacc acccgagcgc attggtgtac
     5221 tcgtagcagt gcatcggcat ggagaagttg ttcaccccat gcgtcgccag cacgatttcc
     5281 gccgccgcga tctggatcac atcgacaatc cgtcgcggat ccttcccggc ccgcagcatc
     5341 cgcgtcacgc cgtccaggct cgctggcgag tgctcatgca gcagcccccg catcacctcg
     5401 aactcttcct ccgcctgaag cgccccggta ttgcgcagca tcgccagctc cgcatccgaa
     5461 acaccgccat acggcaccgc gtgcagcaca acgccctcga tctcttcctt gatcgtgttg
     5521 cacgccatct cgtacagcga gtgccatcgc gggcccaccg ccatatccag cgcgccggcg
     5581 tagatcacat tgtgcgcatt gtcccacccg agcgcgttcc ccagctccac cgtcgagcgc
     5641 gctcgatacg ccttatgtcc cgtcgtatac gagcggttgc ggtacatccg gtcctgcaca
     5701 tcgatcatgc ccgcgaacac cagttccgcc agcacctcct tgcggtccgc cacctcctcc
     5761 atcagaccga ggaacgtccg gtaagcgggt agcacctcgc cccgctgcac caacgtcagc
     5821 cactcgccca gtcgctcctt cagcggctcc cgcgatcgca acggttgctg gtccggccaa
     5881 tagaccaccg gcgcgggggg attctcgatc ttgccgtcga agccacgcgt gtaatgcccc
     5941 gtcgctttgt tgatcttctg gttccagata tcgagcccgg tcgggatgta ccatatcgtc
     6001 tgcgccatcg gcagcatcgc caggccgtcc ggcatcagcc tggccagatg caacgtcgcc
     6061 cgcgccgaca gcaggcaatg gtcgttgttg acgaagttgg gatacccgtc gtctatacgt
     6121 tcgtgatacg gcacgtgcgt gaacggcgcg tggatacgca cgcactccgc gacgatttcg
     6181 ttcagcggac gcgcggcccg gaccaggtca tagaacgcct cgccggcccc cacctggtcg
     6241 cgtgccagga tccgctcctc caacctcgta tactggtgcg caacatcgcc tccgcggccg
     6301 gcgtccgtct ccatctgccg ttcgtcccct tgatgagctc tttgcctgaa ccccaacggt
     6361 tacggttcag ccgggacgat ggctttgatt tagatcaaat ggcagaatta ttaacccgtc
     6421 cgtcagacgg cggcgtggca gaccgcagcg accttgttgc cgtccggatc gcgcacatag
     6481 gcggcatagt agtcggcatg atagttcggc cgcaggccgg gcttgccttc gtcggtcgct
     6541 cccagcccga gtgccttcgc atggaaccga tccaccgcac cgcgcgacgg cgccaggaag
     6601 ctgaccgaca cgccgttccc cacgctggcc ggctggccat tgatcggcag catgacgaag
     6661 aagccgccct cgtggcccga tccccagccg gcaaatccct cgccgctctt gccgcgcgtg
     6721 atcccgagct cccccagcac cgcgtcatag aatgcggtgg cgcgggcaag atcgcgcacg
     6781 cccacggtga tgtggctcag aatgctgctc atgcttcccg ctccggcgag ggaaactcga
     6841 cgccgtcgtg caactcagac aaagcgacct ccgtgccgat ctcgggcata cgcagcaccg
     6901 catcggcatt cagcagctga ccgacccaat cgtcacagct caaagccgtc gaacccgaag
     6961 cgtagctcct ggtgttcctc ccattcgagg aactcctgct tcgtcatcgg ccttcgcgcg
     7021 gcgagttgca tcgagcgact gtagcacgcg gctcattccg ccactatcga tgggcgatgg
     7081 agaccggctc ccccaccgga tcagccgtca ggaagccgag cgtctcgccc tccagcacca
     7141 ggcacgtcat cttgccgccg aacaccgcgc cggtatcgat cgctacgcga ttgtgccgca
     7201 ccaccggctc cttcaccggc gagtgcccat gcaccaccac agcgccgaaa tccatttcgg
     7261 aatacaggaa cggctggcgc atccgcagca ggtcctcccg cgcctgccga tccagcggca
     7321 cgcccggacg cacgccgcca tgcacgaaga aatacccccc ctcgcgatgg gtcagtgtca
     7381 ggttgcgcag gaattccaga tgctccactg gcacactctc cggccagctt tcgcgcggcg
     7441 aatccggatc gatgccgtag ctctccagtg ccggccggcc gccggcgaac agccagtccg
     7501 tcgccgccgg ccgatcgccc gacagcgcat ggagcatcgt ctcgtcatgg ttgccgacca
     7561 gattgaccac ctgcatgccg gacacgacag gatcgcgcag cagccgccgc agcacaccgg
     7621 ccgtgtccgc gccgcgatcc acgtaatcgc cgatatgcag cagcaccggt tcgtcgacgg
     7681 tgcgccgcgc caggtcctcg gcaatcatct cgtgcagatt gccaagctgg atgtcgcagc
     7741 catggatatc gccgatggca tagacacgcc gtcccggcgg caggctcgcg ggcgctctgg
     7801 taaggtcgat cattgcgatc ggaactaaac cggcaacagc ggcagcacca cccggctggg
     7861 ccgtgccgca tcgacgaata ccgtgttgac cgcgacgcgc ttgcgcagcg cgcgtccctc
     7921 cggctcgccg gtgttcgggt tgacgtcgaa cttcgggaag ttgctcgacg acacatccag
     7981 ccggatacga tgccccggca ggaacaggtt cgccgttgga aacagcgtga tgcggatctt
     8041 caccacctcg cccggttcgc gcagccgggg acgcgtcgga tcctcggcat agcgcagccg
     8101 caagatcccg tcggtcagga tcatcgcgta gcccttcgga tagtcggcag atgccggatg
     8161 cacgtcgatc agcttcgcag tgaaatcggt atccggccca tcggtggaga caaacagctc
     8221 agcctcgatc ggccccacca cctgcaccgc gctcttcaat ggcggggtct gaaagaccag
     8281 cacatccctc cgtgacgcca acggcagata aggcggcgaa cagccgagga aatccggccc
     8341 ctccacctga tcccacgccc cggcatatgc caccggatcg aggctgctca tcgccccgcc
     8401 gatcgtcggc accggccgcg acggatcgaa atcgtaggac agcggcgcgg caccaaccgc
     8461 cggcatcaca cgatccagcc cgccgtcacc atgcagatgg aaggcctcga aagcgacgcc
     8521 gggcggcggc cagtccggcg ccgatatcca ccgtccgcca tgctccagct tgccggccgc
     8581 attgcgccgc ccgctgccgc cgcccatgac aaacagccgc accgccggtt catccagcgc
     8641 cgcgcccttc acgatatggt cgaagaaccg cagccggtaa gcgcgccaat ccccggccca
     8701 cgcatccagc ggcgcatttg gcccgaactc cacatcaccg aagctggtgt tgctgcggtc
     8761 gccatgcgtc cacggcccga ggataagccg ctgctgtccg cgcccggcgc gcttcaaccc
     8821 aagataattg cccgtcgccg tcagcggata cgggtcccac cacgaagaca tgtgtacgca
     8881 cgccgcgcgg ctgtagcgtt cgtgccagcc cgcggtccag attccgagct gctgccagaa
     8941 ctcatcgaac gcaccgtgcg tccactggcc gaacaggtac gcctcgtaat ccggatggtg
     9001 ccgcagcggc gaatggccag gcttccacgg catgcgcatg aaccac
//
LOCUS       DATGEM010000652        10198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1072720, whole genome shotgun sequence.
ACCESSION   DATGEM010000652 DATGEM010000000
VERSION     DATGEM010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072720"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..466
                     /locus_tag="VKQ27_20105"
     CDS             62..466
                     /locus_tag="VKQ27_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91299.1"
                     /translation="MRIAGWSLDGVCSPQIGQNVCQFPPLPPPNHRVSAETTKKGVPH
                     MLQSMTWLANDKIARDQFQVFSSIGDEMQLSDDDQRRVLLLSDQQWADWSDFLQDGPL
                     PAEPQLPVMLRRLGSASHRLAVLADRRASASA"
     gene            521..775
                     /locus_tag="VKQ27_20110"
     CDS             521..775
                     /locus_tag="VKQ27_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLY91300.1"
                     /translation="MRAIFVQIKCEMGQAYRVAREAADTIEEMSELYSTSGQYDLLGK
                     FYLEAEQDIGLFVTEKVQTLPGVKDTYTMITFNAFSGGRG"
     gene            complement(924..1316)
                     /locus_tag="VKQ27_20115"
     CDS             complement(924..1316)
                     /locus_tag="VKQ27_20115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY91301.1"
                     /translation="MSEPLFHMIAGAPTPVAPFSHAVETDGWVFLTGQMPTLPGDDSA
                     NLPEGIQAQTRRVMDNLIIVLQGLGLGLQHVVSVRAYLTHFDRDYAPMNEAYRSYFPA
                     DRLPARTCIGVTGLARGALVEIDLVARR"
     gene            complement(1313..3613)
                     /locus_tag="VKQ27_20120"
     CDS             complement(1313..3613)
                     /locus_tag="VKQ27_20120"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLY91302.1"
                     /translation="MATMQSKSVRRREDAKLLTGRGNYSADGHKPGMLHAVLVRSHHA
                     HAVINGIDTSAARALPGVVAVFTGADLTDVAPIPGGIGFPRPDGGPAPKTNRPLLAAD
                     RVRFVGEPVAVVLSETMEAGKDAAEAVMVDYRELPLVTDAVAALEPDAPKVWDDVPDN
                     IGFLWKRGDVAAADDALRNAAHVARLQFTVSRVTANSMEPRGAWAEIDSDGRMVVHAA
                     HQSPFNLRNGMANSNFNIKPTDIRVLCEDVGGSFGMKSGVQPEYVLAAWAARKLQRPV
                     RWIEDRTEGFLTDEQAREMRITVELALDATHKFTALRLRWDVNLGAYVTGRSGWPVGN
                     IGGTAGVYDIPAIYAESCGILTNTVPTAAYRGAGRPEATYTIERIIDIAARELGVNPY
                     ELRRRNLIPPEAMPYKTALTFTYDCGEFEGNMQKASELAELDSFAARRAEAEGRGKLR
                     GIGLCNCIEVAGGPFLRPAKDQAALRLAADGTLVLRSGSMSVGQGLETAFTQLVADRF
                     GVSQDSVRYEAGDTDMLPGGKGNGGSGALCIGGAAVDLVVNKVIDKAKQVAAELLEAA
                     DVDVVFEAGRFTIAGTDRSVTLAEVAQAAVIDGNELVESGEFAATAVTFPNGTHICEV
                     EIDPDTGVTEVVRYSAVEELGRVLNPMLVAGQVHGGVVQGVGQAMVEQIIHDPQSGQM
                     LTASFMDYIMPRADDLPEIAMATREVPTKVNPLGAKGVGEAGTVGAMAAAMNAVNDAL
                     APLGIRHFDMPATPYRVWKAIAEARA"
     gene            complement(3613..4593)
                     /locus_tag="VKQ27_20125"
     CDS             complement(3613..4593)
                     /locus_tag="VKQ27_20125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLY91303.1"
                     /translation="MTYKVAFLDVMDSKVQAEIRSELPADFAIDFAETLDRREHMTMI
                     RDADFILTTLAVDAEMIRSAPKLKLLHKWGIGVDKFDLDAARAARVPVAITFGANAGA
                     VSEHTLMLMLATYRRLALADRKLRDGVWLRSQLRAQAYQLSGKTVGLFGFGNVGRMVA
                     HRLAGFDVEILYHDIRRAEMAVERSLRATPVPFEELLARSDVVSVHVPLTKVTRGMVG
                     AAAMAQMKTGAIMINAARGEIVDEAALYDALVSGKLHGAGLDVYATEPADAGNPLFKL
                     DQVVVTPHTAGSVIDLVADIARHAFNNMQSVLSGRPLSPNDVIVAAGDNR"
     gene            4671..5474
                     /locus_tag="VKQ27_20130"
     CDS             4671..5474
                     /locus_tag="VKQ27_20130"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY91304.1"
                     /translation="MAEELRDILRNRVKEKLARDEVVASMTVRLSRSIEIARIAATAG
                     FDTIYVDVEHNTLSIDAVCQICIAAMQCGITPLVRVPANTPEFICRLLDGGAMGVICP
                     HVRTAEEAREIVRYAKFTPQGERGAGGALPHYHYRSFPSAALNQAMNDATMVVVMMET
                     VAALENVEAIAAVDGIDMMLIGTNDLCNEMGITGQFDHPNVRDAFLRTIAACRKHGKH
                     VGVGGLASRPDLMAEYVRQGARYVSTGTDLAFLIAAATEKAKFVRDIKG"
     gene            complement(5464..6576)
                     /locus_tag="VKQ27_20135"
     CDS             complement(5464..6576)
                     /locus_tag="VKQ27_20135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY91305.1"
                     /translation="MTRRLCVAVVLLICASPALATMTQLPGLVREPLRLAITLPDGTT
                     AELEALVTRPDGPGRFPLALINHGLPRNASAAVRMTPETYSSPAIVFAQHGYAAVVVN
                     RRGFGLSSASIDLPMGPCGDRDYTEAARAQAVDILAALGSLRQEPWVDPEHIVLVGHS
                     LGGLAALAAATDAPAGVTGIIDFAGLLGSAMTGMVCSPQHLVATMHELGAATHIPSLW
                     IFAQNDGFFGPAIARQTFDAYTAAGASAEFDAAPPYGHNGHLLIFSPPPVVWWPRVAG
                     FLEQLHLPTRLVDDLPSPADLPEPPNLDTRGKQDLADYVHTRDYEKAFATDGKGHYAN
                     IIAQRTQEDAEAAALAHCKSRGWACKIYAVGNTLSP"
     gene            complement(6573..7457)
                     /locus_tag="VKQ27_20140"
     CDS             complement(6573..7457)
                     /locus_tag="VKQ27_20140"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HLY91306.1"
                     /translation="MIGSLSRETPVIVQGITGRMGRTHAALMRAYGTNIVGGTSTRVD
                     QAEAAGVPVFANCRDAVLATGALASVAMAPALETLAAVEEALAAGIRLIVTIAEGIPQ
                     HDALRIGRAVRRANATWIGGSTPGMAIPGEVKLGFIPDVCLRPGSFAIMTKSGTLSYE
                     VGYRLVQVGLGQSIWVGVGGDPVKGVRFADLLPVFFAHEPTQAVILVGEVGGSEEEEC
                     ADALRRLGVRKPVYALIAGREAKEGVSMGHAGALVMGEAGSLASKTERLTGAGARVFG
                     SVEAIVEACAADFATPAA"
     gene            complement(7454..8551)
                     /locus_tag="VKQ27_20145"
     CDS             complement(7454..8551)
                     /locus_tag="VKQ27_20145"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HLY91307.1"
                     /translation="MLLIEVDGKALLADHGIAVPDGVLVVDTTIGTLPSDGPWIVKAQ
                     VPVGGRGKAGGVARVDTPQDAAAAVHRMLGQRLKGHQVEACLIEQAAAGEEHYLSVMI
                     DAASYGLRVIYSAQGGVDIEQSGSARGRPCPPHPAAVAEALGDLGAEARIVAVGRALA
                     DMLLQRELSLAEINPLFVSSDGVIAGDAKLAIDLDALPRQPRIAALIEARPTIYADAN
                     RKLSEGFDYVELDPDGEIGLVTTGAGLSMMLIDEMAARGLKPLNFCDIRTGQMRGSPA
                     RLMRVLEWMTARASLRAVLVNVFAGITDLAEFGSLLASGIEQSPQLRVPVIARLVGRN
                     AAEAKRILAERQPNVLVTEDLEEALRALLPA"
     gene            complement(8553..9719)
                     /locus_tag="VKQ27_20150"
     CDS             complement(8553..9719)
                     /locus_tag="VKQ27_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY91308.1"
                     /translation="MDFHISPEQRQMLASVRELAQAEFKPKAARWMDGSFPWENMKQL
                     ASLGVLGMSVPEEYGGLGLPILDTALILEEIAKVCYVTAMAVLGEAGVQTRVIARYAP
                     ASIRDRILPRVVSGDCMLAICMTEPHAGTDVANYRTNARVVGDKVILKGTKTLISRAP
                     EAGVFIIFSRIDGRPGREGIGCILLEAGTPGFEVTGTYHTMGGENLFEIQFNDCELPL
                     ENLVIKDDGFRRLLTAFNTQRCLNPAISLGLAEGAFDEAVNYVRERTVFGKPIADHQG
                     IRWKLADMFKDIEAGRGILYRACLTANPFPDPFMAAAAKVFCNEMSLRVTSEAVQLHG
                     GFGFTDEAPVSRLYRGARYGTIGGGTSETLRDLMGRKIVGDLDFDQGIMGLGTY"
     gene            9839..>10198
                     /locus_tag="VKQ27_20155"
     CDS             9839..>10198
                     /locus_tag="VKQ27_20155"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLY91309.1"
                     /translation="MAPTGSVAHKLASIEAVEQGLASVGYIPSRQISTAVYLAQHLEK
                     PILVEGPAGVGKTELAKSVAAFLDLTLIRMQCYEGLDESKALYEWKYGKQLLYTQILK
                     DKIGDVIGATETLPAALT"
BASE COUNT         1955 a         3573 c         2936 g         1734 t
ORIGIN      
        1 gagccagttc cgtcacgccg acggcgcctg taactcgtac gtagaatccc ggatcgtata
       61 aatgcgaata gccgggtggt cactcgacgg tgtttgctcg ccacaaatcg gacaaaacgt
      121 ctgccaattt ccgcccttgc ctccaccgaa ccatcgagta tctgctgaga cgacgaaaaa
      181 aggggtgccg catatgcttc agagcatgac ctggttagca aacgacaaga tcgcccggga
      241 ccagttccag gtgttctcca gcatcggtga cgaaatgcaa ctcagcgacg acgatcaacg
      301 gcgcgtgttg ttgctgtcgg accagcagtg ggccgactgg tccgatttcc ttcaggatgg
      361 tccgctgcct gccgaacctc agcttcccgt gatgctgcgc cgcctcggca gcgccagcca
      421 ccgcctcgcc gttctggccg accgccgcgc ctccgcctca gcgtagttcg ccccccggca
      481 aatcctgcta cgctgcctga gcggcgtggc aggaggcgac atgcgagcga tcttcgtcca
      541 gatcaaatgc gagatggggc aggcctaccg ggtcgcccgt gaagctgccg acaccatcga
      601 agagatgtcg gaactttact ccacctccgg tcagtacgac ctgctgggca agttctacct
      661 ggaggctgag caggacatcg ggttgttcgt caccgagaag gtgcagacgc tgcccggggt
      721 gaaggacacc tacacgatga tcaccttcaa tgccttcagc ggtggccgcg gctaggcctg
      781 cgggcccttg tccgcctctg cccccaggct cgcccttacc ccttccccat ccctcctccc
      841 tgtcattgcg accccgaagg ccgcgggaag caatctcgta gagcctggcg cgcaaggcca
      901 cccggctgct tcgcaacgac acattatcgc cgcgccacca gatcgatctc caccagcgcg
      961 ccacgcgcca gacccgtaac gccgatgcag gtccgcgccg gcagccgatc ggccggaaag
     1021 tagctgcggt acgcctcatt catcggcgca tagtcgcgat cgaagtgcgt caaataggca
     1081 cgcacgctga caacgtgctg caaaccaagc ccgagtccct gcagaacgat gatcagattg
     1141 tccatcacgc gccgcgtctg tgcctgaatg ccctctggca aattcgccga atcatccccg
     1201 ggtaaagtcg gcatctgccc ggtcaggaac acccagccat ccgtttccac cgcgtgcgag
     1261 aacggcgcca ccggcgtcgg cgcgccggcg atcatgtgga acagaggctc gctcacgccc
     1321 gcgcctcggc gatcgctttc cacacgcgat acggcgtcgc cggcatgtcg aagtggcgga
     1381 tgccgagcgg cgccagcgca tcgttcaccg cgttcatcgc agcggccatg gcaccgaccg
     1441 tgccggcctc gccgacgcct ttggccccca gcgggttcac cttggtgggc acttcccgcg
     1501 tcgccatcgc gatctccggc agatcgtccg cgcgcggcat tatgtagtcc atgaacgacg
     1561 ccgtcagcat ctgcccggac tgcggatcgt ggatgatctg ctccaccatc gcctggccaa
     1621 cgccctgcac gacgccgccg tgcacctggc ccgccaccag catagggttc agcacccggc
     1681 caagctcttc cactgcgctg tagcgcacca cctcggtgac accggtatcc ggatcgatct
     1741 ccacctcgca gatatgcgta ccgttgggga acgtgacggc ggtggcagcg aactcgccac
     1801 tttccaccag ctcgttgcca tcgatcaccg cggcctgcgc gacttcggca agggtaaccg
     1861 aacgatccgt accggcgatg gtgaaacggc cagcctcgaa caccacatca acatccgcgg
     1921 cttccagcaa ttcagccgcc acctgcttcg ccttgtcgat gactttgttc actacaaggt
     1981 cgaccgcggc gccaccgatg cacaacgcac ccgatccacc attgcctttg ccaccgggga
     2041 gcatatcggt gtcgccagcc tcatagcgca cgctgtcctg cgacacgccg aatcgatccg
     2101 ctaccaactg ggtgaatgcc gtctccaacc cctgccccac cgacatcgac cccgatcgca
     2161 gcaccagcgt gccatccgcc gccaggcgca acgctgcctg gtccttcgca ggacgcagga
     2221 aaggcccacc cgcgacctca atgcagttgc acaggccgat gccgcgcaac ttgccgcgtc
     2281 cttccgcctc ggcacggcgc gcggcaaagc tgtcaagctc cgccagttcc gacgccttct
     2341 gcatgttgcc ttcgaattcg ccgcaatcat atgtgaaggt aagcgcggtc ttgtacggca
     2401 tcgcctcagg cgggatcagg ttgcgacggc gaagttcgta aggattgacg ccaagctcgc
     2461 gtgccgcgat atcgatgatg cgctcgatcg tataagtagc ttcaggacga ccggcaccac
     2521 gatacgccgc ggtgggcacg gtgttggtca ggatgccgca cgactctgcg tagatcgctg
     2581 gaatgtcata aacgcctgca gtaccgccaa tgttgccgac cggccaaccg gaccggcccg
     2641 tcacataggc acccaggttc acatcccagc gcagcctcag cgcggtgaac ttgtgcgtcg
     2701 catccagcgc cagttcgacc gtaatgcgca tctcgcgcgc ctgctcatct gtcagaaagc
     2761 cctcggtgcg gtcctcgatc caacgcaccg gccgttgcag cttgcgcgcc gcccaggccg
     2821 ccaacacata ttccggttgc acaccggact tcatgccgaa cgagccaccc acatcctcgc
     2881 acagcacgcg gatatccgtc ggcttgatgt tgaagttgct attcgccatg ccgttgcgca
     2941 gattgaacgg cgactggtgc gcagcatgca ccaccatgcg gccatcgctg tcgatctccg
     3001 cccacgcgcc gcgcggctcc atcgaattgg cggtgacacg ggaaaccgtg aactgcagcc
     3061 gtgcgacatg ggccgcattg cgcaacgcat cgtccgctgc agccacatca ccgcgcttcc
     3121 acaggaagcc gatgttgtcg ggcacgtcat cccagacctt tggtgcgtct ggctccaacg
     3181 cagccacggc atcggtgacc agaggcagct ctcggtaatc caccatcacc gcttctgctg
     3241 catccttgcc ggcttccatg gtctcggaca gcaccaccgc caccggctcg ccaacaaaac
     3301 gcacgcgatc cgccgccagc agcggccgat ttgtcttcgg cgccggaccg ccgtcaggtc
     3361 gaggaaaccc aatgccgcca gggatcggcg ccacatcggt cagatcggca ccggtgaaca
     3421 ccgcaaccac gcccggcaac gcacgcgccg cgctggtatc gatgccgttg atcaccgcat
     3481 gggcatggtg cgagcgcact agaacagcat gcagcatgcc tggcttgtgc ccgtccgcgc
     3541 tatagttgcc ccttccggtc agtaacttgg catcctcgcg acgacggacc gacttgctct
     3601 gcatcgttgc catcacctgt tgtcccctgc tgcaacaatc acgtcattgg gcgacagcgg
     3661 acggccgctc agcacggatt gcatgttgtt gaacgcatgg cgcgcgatat cggcaaccag
     3721 gtcaatgacg ctgccagcgg tgtgcggcgt caccaccacc tggtccagtt tgaacagcgg
     3781 attgcccgca tcagccggtt ctgtcgcgta aacatccagc cctgcgccgt gcagcttgcc
     3841 gctgacgagc gcatcataaa gcgccgcttc atcgacgatc tcaccacgcg ccgcattgat
     3901 cataatcgcg ccggtcttca tctgcgccat cgccgcggca ccgaccatgc cgcgcgtgac
     3961 cttggtcagc ggcacatgga ccgacacgac gtcgctgcgc gccaacagct cctcgaacgg
     4021 caccggtgtc gcgcgcaacg acctctcgac cgccatctcg gcgcggcgga tgtcgtgata
     4081 caggatctcc acgtcgaagc cagccagccg gtgcgcgacc atccgtccga cattgccgaa
     4141 cccgaacagc cccaccgtct taccggacag ctgatacgcc tgcgcccgaa gctgcgaccg
     4201 cagccacacc ccatcgcgca gcttccgatc ggccagcgcc agccgccggt acgtcgccag
     4261 catcagcatc agcgtgtgtt cggaaaccgc accggcattg gcgccgaagg tgatcgcgac
     4321 cggcacccgg gcggcacgcg ccgcatccag gtcgaactta tcaacgccga tgccccattt
     4381 gtgcagcagc ttcagcttcg gcgccgagcg gatcatttcg gcatcgacag ccagcgtggt
     4441 caggatgaaa tcggcatccc tgatcatcgt catgtgttcg cgccgatcga gagtttcggc
     4501 gaaatcgatg gcaaaatcgg ccggcaactc cgaccggatc tcggcctgca ccttggaatc
     4561 catcacgtcg agaaaggcaa ccttgtaggt catgcggcac tccccatgcg caccatcttg
     4621 aggcccatgc gccctgcgag caacaatggc gggcaattcg ggaggaaccc atggcagagg
     4681 aactgcgcga catcctgcgc aatcgcgtga aagagaagct ggcacgcgac gaggtcgtgg
     4741 catcgatgac cgtgcggctg agccgctcga tcgaaatcgc gcgtatcgcc gctaccgccg
     4801 gcttcgacac gatctacgtt gacgtcgaac acaacacgct gtcgatcgac gccgtctgcc
     4861 agatctgcat cgccgccatg caatgcggca tcacaccgct ggtgcgcgta ccggccaaca
     4921 cgcccgaatt catctgccgc ctgctggacg gcggcgctat gggcgtgatc tgcccgcacg
     4981 tgcgcaccgc cgaagaggcc cgcgagatcg tccgctatgc caagttcact ccacagggcg
     5041 aacgcggcgc cggcggcgcg ctgccacatt accactatcg cagcttcccc tccgcggcgc
     5101 tgaaccaggc gatgaatgac gccaccatgg tggtggtgat gatggaaact gtcgccgcgc
     5161 tggagaatgt ggaagcgatc gccgcggtgg acggcatcga catgatgctc atcggcacca
     5221 acgatctatg caacgagatg gggatcaccg gccagttcga ccatccgaac gtgcgtgatg
     5281 cgttccttcg caccatcgcg gcatgccgca aacatggaaa gcatgtcggc gtcggcggct
     5341 tggcatcacg gccagacctc atggcggaat acgtgcgaca gggcgcgcgc tatgtgtcca
     5401 ccggcacgga cctggcgttc ctgatcgctg ccgccacaga gaaagcgaag ttcgtgcgcg
     5461 acatcaaggg ctgagcgtgt tccccaccgc ataaatcttg caggcccagc cgcggctctt
     5521 gcaatgtgca agcgccgcgg cctccgcatc ttcctgggtg cgctgggcga tgatgttggc
     5581 gtagtggccc ttgccgtccg tcgcgaaggc tttctcgtaa tcgcgggtgt gcacgtagtc
     5641 cgcaaggtcc tgtttgccac gcgtatcgag attgggtggc tcaggcagat cggccggcga
     5701 tggcaggtca tcgaccagcc gcgtcggcag atgcaactgc tcgaggaacc cggcaacacg
     5761 cggccaccac accacaggcg gtggcgagaa aattagcaga tggccattgt ggccgtatgg
     5821 gggtgccgca tcgaactccg cggacgctcc tgcggctgta tatgcgtcga acgtctgccg
     5881 tgcgatggct ggcccgaaga acccgtcgtt ctgcgcaaag atccacaggc ttggtatgtg
     5941 cgttgcggca cctaattcgt gcatcgtcgc aaccaggtgc tgcggactgc acaccatgcc
     6001 ggtcatcgcg gagccaagaa ggccggcgaa atcaataatg cccgtcacgc ccgctggcgc
     6061 atctgtcgct gcagcgagtg cagccaggcc accaagggaa tgcccaacga gaacgatatg
     6121 ttccgggtcc acccacggct cctgacgcag cgatcccagc gcggccagaa tatcgaccgc
     6181 ctgtgcacgc gccgcctcgg tgtaatcccg gtcgccgcac ggccccattg gcaggtcgat
     6241 cgatgccgac gaaagaccga agccgcggcg gttcaccacc accgcggcat agccatgctg
     6301 cgcaaacaca atagccggag acgaataggt ctccggggtc attcgaaccg ccgcgctggc
     6361 gttacgcggc agtccatgat tgatcagcgc cagcgggaag cggcccggcc catcgggacg
     6421 cgtcaccaga gcctccagtt cggccgtcgt gccatccggt agcgtgatgg ccaaccgcag
     6481 cggctcgcgc accagtccgg gaagctgcgt catcgtggcg agcgcggggc tcgcgcagat
     6541 caacagcacc acggcgacgc acaggcgccg ggtcatgcgg ccggcgttgc aaagtccgcg
     6601 gcgcaagcct ccacgatcgc ttccaccgaa ccgaacacgc gcgcacccgc cccggtgagg
     6661 cgctcggtct tcgacgcgag tgagccggcc tcccccatca cgagagcacc ggcatggccc
     6721 atcgagaccc cctccttcgc ctcgcgccca gcgatcaagg catagacggg cttgcgcacc
     6781 ccaagacgcc gcagcgcgtc ggcgcactct tcctcctcgc tgccgcctac ctcgcccacc
     6841 aatatgaccg cctgggttgg ttcgtgcgcg aagaataccg gcagcagatc ggcgaagcgc
     6901 acccctttca ccggatcgcc accgaccccg acccagatgc tttgccccag tcccacctgc
     6961 accaaccgat agccgacttc gtaggaaagc gtgccgctct tggtcatgat cgcgaaggac
     7021 cctggccgca aacagacatc ggggatgaag cccaacttga cttcgcccgg aattgccatg
     7081 ccgggcgtgg agccgccaat ccatgtggcg ttcgcacgac gaaccgcacg accaatgcgc
     7141 aatgcgtcat gctggggaat tccttccgca atggtgacga tcaggcgaat gccggcagca
     7201 agcgcttctt ccacggcggc aagtgtctcc agcgccggcg ccattgcgac cgatgccaga
     7261 gcaccggtcg ccagcacagc gtcccggcag ttggcgaata ccggcacacc agccgcctcc
     7321 gcctggtcaa cgcgcgtaga tgttccaccg acgatgttgg tcccataggc gcgcatcagc
     7381 gcggcgtgcg tgcggcccat gcgcccggtg atgccctgca cgatcaccgg cgtctcgcgc
     7441 gacaacgacc cgatcatgcc ggcaacagtg cgcgcagcgc ttcctccagg tcttcggtaa
     7501 ccaatacgtt gggctgtcgc tctgccagga tgcgcttggc ctccgctgca ttgcggccaa
     7561 ccagccgcgc gatgacgggc acgcgcaact gcgggctctg ttcgatgccg ctcgccagca
     7621 gactgccgaa ctcagcaaga tcggtgatgc cggcgaacac attcaccagc accgcacgca
     7681 acgacgcccg cgccgtcatc cattccagca cgcgcatcag ccgggctggg ctgccgcgca
     7741 tctggccggt gcggatgtca cagaaattca gcggcttcag tccgcgcgcc gccatctcat
     7801 caatcagcat catcgacagg ccggctcccg tggtcaccag gccaatctcg ccgtccggat
     7861 cgagctccac ataatcaaag ccctcactca gcttgcggtt ggcatccgca tagattgttg
     7921 gtcgcgcctc gatcagcgcc gcaatccgcg gttggcgcgg cagcgcgtcg agatcgatcg
     7981 caagcttcgc atcacccgcg atgacgccat ctgacgagac gaataaagga ttgatctccg
     8041 caagagacag ttcacgctgc aacagcatgt ccgccagggc gcggcctacc gcgacgatgc
     8101 gcgcctcggc cccaagatcg cccagtgctt cggcgaccgc ggcgggatgc ggaggacaag
     8161 gccgtcctcg cgcagaaccg gactgctcga tgtccacgcc accctgtgcc gaatagatga
     8221 cgcgcagtcc gtagctggca gcgtcgatca tcacagacag ataatgttct tcgccggccg
     8281 ccgcctgctc gatcaggcag gcctccacct gatgaccctt caggcgctgg cccagcatcc
     8341 gatgcacggc agcagccgcg tcttgcggcg tgtccacgcg tgcgacaccg ccggctttgc
     8401 cacgaccacc gaccggcact tgcgccttca cgatccacgg gccgtcgcta gggagagtgc
     8461 caatcgttgt gtcaacaacc agcacgccgt ccggcaccgc gatgccgtgg tcggccagca
     8521 gtgccttgcc atccacttcg atcagcagca cctcagtacg tccccagacc catgatcccc
     8581 tgatcgaagt ccaggtcgcc gacgattttg cgacccatca ggtcgcgcaa cgtctccgag
     8641 gtgcctccac cgatcgtgcc ataacgcgcc ccgcgataca gccgcgacac cggcgcctca
     8701 tcggtaaacc cgaacccgcc gtgaagctgc acagcctcgg atgtcacgcg cagcgacatc
     8761 tcattgcaga acactttggc tgccgccgcc atgaacggat cgggaaacgg attagcggtc
     8821 aggcaggcac ggtacaggat accgcgcccc gcctcgatat ccttgaacat gtcggcaagc
     8881 ttccatcgga tcccttgatg atcggcgatt ggcttgccga acacggtgcg ctcacgaaca
     8941 taattcaccg cctcgtcgaa cgctccttcc gccagcccca acgagatcgc ggggttcaaa
     9001 cagcgctgcg tattgaacgc ggtcagcagc cggcggaacc catcatcctt gatcaccagg
     9061 ttctcaagcg gcaactcgca gtcgttgaac tggatctcga acaggttttc gccgcccatt
     9121 gtgtggtacg tgccggtcac ttcgaatccc ggcgtgcctg cctccagcaa gatgcagccg
     9181 atgccctctc gcccaggcct gccgtcgata cgactgaata tgatgaatac gcccgcctct
     9241 ggcgcgcggc tgatcagcgt cttggtgccc ttcaggatca ccttgtcgcc aaccacccgc
     9301 gcattggtgc gatagttcgc gacatcagtg ccggcgtgcg gctcggtcat gcagatcgcc
     9361 agcatgcaat cgccgctgac cacgcgcggc agaatgcgat cccggattga ggccggcgcg
     9421 taacgggcaa tcacgcgcgt ctgcacaccc gcctcaccca gcacagccat cgcggtgacg
     9481 taacagacct tcgcgatctc ctccaggatc agcgcggtat ccaggatcgg cagacccagg
     9541 ccgccgtact cctccggcac cgacatcccc agtactccca gcgaggccag ctgcttcatg
     9601 ttctcccagg ggaacgagcc gtccatccag cgtgcggctt tgggcttgaa ctccgcctgc
     9661 gccaactcgc gcaccgaggc gagcatctgc cgctgttccg ggctgatgtg gaaatccatg
     9721 ccgttccgtc ccatttgcgt tcggcgagat tagcggcgat cccccctttg gcaatcgcgc
     9781 cgcccaatgc tacccatgag tagaatcatc gattggaacg tgcatgccag acgcaggagt
     9841 ggcgccgacc gggtcggtcg cgcacaaatt ggcctcgatc gaggccgtgg agcaggggtt
     9901 ggcctctgtc ggctacatcc ccagccggca gatctccaca gccgtgtatc tcgcccagca
     9961 cctggaaaaa cccatcctgg tggaaggccc ggcgggcgtc ggcaagaccg aactcgcaaa
    10021 gtccgtcgcc gcctttctcg acctgacgct gatccgcatg cagtgctacg agggcctcga
    10081 cgaatctaag gcactctacg agtggaaata cggtaagcag cttctctaca cccaaatcct
    10141 gaaggacaag atcggcgatg tgatcggcgc cacggaaacc ctgcccgccg ccctgacc
//
LOCUS       DATGEM010000653         8005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_165860, whole genome shotgun sequence.
ACCESSION   DATGEM010000653 DATGEM010000000
VERSION     DATGEM010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165860"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VKQ27_20160"
     CDS             <1..554
                     /locus_tag="VKQ27_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine acetyltransferase"
                     /protein_id="HLY91310.1"
                     /translation="NQVFLRSLMATLEAAPEHTGDGRFSAEPRAAKRAFGRIYAAWAL
                     SQDFYRAGLHLASGPQPNLGAPDLETFLRTDWEERFGVRPAANAYAQLRTWDAADISA
                     NSLYDDDLARALRAIRARVLLMPGETDLYFRVADNTAELRYLSQAELKPIPSVWGHRA
                     GNPTTNPADARFVKDAVRDWLAR"
     gene            574..960
                     /locus_tag="VKQ27_20165"
     CDS             574..960
                     /locus_tag="VKQ27_20165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY91311.1"
                     /translation="MAMITANNPSTVRAPTGYTHSMLIEGEQRRLIMSGQVGMALDGS
                     VPATGEGQITQAFANLRALLEANGMGVTNLVKTTVFLTDRSLLASFRAARDAVYGGHA
                     PCSTLLFVAGLADPRFVVEIEAEAVA"
     gene            complement(963..2012)
                     /locus_tag="VKQ27_20170"
     CDS             complement(963..2012)
                     /locus_tag="VKQ27_20170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLY91312.1"
                     /translation="MLSPAPSYRILLVTDAWSPQVNGVVRTLTTVVNELRAMGHIVEV
                     IAPDRFRTIPCPTYSDIPLALLPRRSMVRMIEAFRPDAMHISTEGPLGMAARSWAMRN
                     GFHFTTAFHTRFAEYIQARIGLPVGPIYAWMRRFHSAGEGTMVATQSLRDELSKRGFR
                     NIRSWSRGVDLDLFKPEPREDWGLPRPMFIYVGRVAVEKNIGAFLDLDLPGSKVVVGG
                     GPILPKLQRDYPSVHFTGPRYGEALARAYAGADVFVFPSLTDTFGLVMLEALACGTPV
                     AAFPVTGPIDVLAEAHGSVGAVNADLRAAALEALTADRAACRAHAERYSWRACAETFL
                     SHLVPIAGAHARLAG"
     gene            complement(2050..2862)
                     /locus_tag="VKQ27_20175"
     CDS             complement(2050..2862)
                     /locus_tag="VKQ27_20175"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLY91313.1"
                     /translation="MNAPIVPGARPLYRSVFVSDTHLGTRGARCDFLADFLRRITCQN
                     LFLVGDIIDGWRLRKSWYWDESHDEVLRLILRFARSGTHVTYIPGNHDELFRKWLPME
                     IAGIRLRQEAEHITADGKRLLIMHGDEFDSVVRYAKFLALLGDWAYTFALTFNRYFNA
                     VRRRLGYPYWSLSAWLKRQVKEAVKAIDRFEGALAAEARRRGFDGVVCGHIHHAQMHE
                     VQGVLYLNDGDWVESCTALVEHHDGRLELIDWAELNRLSFFTPRRTAVREAA"
     gene            3041..3910
                     /locus_tag="VKQ27_20180"
     CDS             3041..3910
                     /locus_tag="VKQ27_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010665861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HLY91314.1"
                     /translation="MIVVFNPVAGLRRASLLWRVLDVLVANGVRIELAETHRAGHAEA
                     LAREAVARGAQMVVAAGGDGTIAEVANGLMGSPARLGVIPLGTANVMAHEMALPFEPR
                     SVAAALAFGRTRPLWPGVARTGESSRLFVQMLGVGLDAQVVHNLPAPLKRALGRSAYA
                     LQTLREIAFYPYRPIRVRLDGEETQATSVIVSKGRLYGGRFLLAPTACAGEPGFSVVL
                     FDRAGMRWALTYGAALPFNLLAHAPGVRHVRARCIEFIGNESVPAQADGDAAGRTPMS
                     ITDAPSAIPVVVG"
     gene            complement(3913..5718)
                     /locus_tag="VKQ27_20185"
     CDS             complement(3913..5718)
                     /locus_tag="VKQ27_20185"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HLY91315.1"
                     /translation="MTAVDPIPYSDAKIRHILSSVRTIAMVGASSNWNRPSYFVMKYL
                     QGKGYRVIPVNPGTAGQEQLGEKIYASLRDIPDKIDMVDIFRASDQVGPIVNDAIAIG
                     AKVVWMQLGVRNDEAAAKAEAAGLEVIMNRCPKIEFGRLGGELSWSGVNSGIINNRAP
                     QAPTARKAKPRTQPSANVGYGFETRAVHAGAAPDPTTGARTTPIFQTAAYVFDDVDHA
                     ASLFNLHNFGYIYSRLTNPTVAVLEERVASLEGGRAACAAASGHAAQFLIFATMMEPG
                     DEFLASRNLYGGSLTQFGLSFKKLGWTCHFVDPTDPENFRRALTPRCKAIFLELLANP
                     GGIIVDIEAVAKIAHEAGIPLIVDNTLATPYLCRPFEWGADLVVHSTTKFLDGHGNSL
                     GGVVVESGKFDWAASGKFPALTEPEPAYHGLRFYENFGDFAFTTKARAVALRDYGPTM
                     APMNAFLTITGIETLHVRMERHCANAQKVAEFLHGDPRVAWVSYAGLESSPYHSLARK
                     YLPRGAGAVFTFGVSGGYEAGVKLVENVGLFSHLANIGDTRSLVLHPASTTHRQLTDE
                     QRQAAGAGPDVIRLSIGLETAEDLIADLDQALTSA"
     gene            complement(5891..6838)
                     /locus_tag="VKQ27_20190"
     CDS             complement(5891..6838)
                     /locus_tag="VKQ27_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HLY91316.1"
                     /translation="MSKQTRNVLRRTSTLLAAFVALAVVFAPGLADARLGGGGSFGSR
                     GSMTWSAPPATRTAPYSAAPMQRSITPNNPSPGYGSGAYGSSFGPRSGFMSGLLGGLI
                     GAGIGGLLLGHGFFGGMMGFGGFLGFLLQIVLIVVVVRWLIRMFSGASRPAFAGGPNV
                     FARSAAPAAMGGGGGSAAAAPPIQIGPQDYQAFEQVLRDTQAAWSRHDLNALSATTTP
                     EMLSYFSEQLSEQTSQGVRNEVTDVRLLQGDLAQAWSEGSREYATVAMRFSMIDVTRD
                     RTGRIVDGSPDEHVTATELWTFVRSPGSRWILSAIQQAR"
     gene            complement(6864..>8005)
                     /locus_tag="VKQ27_20195"
     CDS             complement(6864..>8005)
                     /locus_tag="VKQ27_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HLY91317.1"
                     /translation="IVSSTTVDPSSFQALAERAVAMARVVPEDPYAGLADTAAPPGTL
                     ALDLVDATEPSTKGLVARAAAAEEAALAVPGITNSEGAEAGYGRSEAYLVTSAGFAGQ
                     NARTSHSVSASALAGSGTDMQRDYDYHSTVHLADLDDPAGIGRSAGERAVTRLHPTRP
                     ATAKLPVVYDQRVAGSLLGHLSGAINGASVARGTTFLKDKLGARIFRDDIEIRDDPRR
                     VRGLRSRLFDGEGTPTAARDLIAAGVLTTWLLDSRSARQLGMVSTGHAARGTGGPPSP
                     STTNLYLTAGTLTPAELMADITLGIYVTELIGMGVNGVTGDYSRGASGFMIRNGALAE
                     PVAEITIAGNLLDMFAHLTPANDLRFRRGTDSPTVRVEGMTLAGA"
BASE COUNT         1375 a         2404 c         2801 g         1425 t
ORIGIN      
        1 acaaccaggt ctttctgcgc agcctgatgg cgacgctgga ggcggcaccg gagcataccg
       61 gagatggccg cttctccgcc gaaccacgcg cggcgaagcg ggcgttcggg cgtatctatg
      121 cggcctgggc gctgagccag gatttctatc gggcggggct gcatcttgcg tctgggccgc
      181 agccgaatct tggagcgccg gacctggaga cgttcctgcg tacggactgg gaggaacggt
      241 tcggcgtgcg gccggcggcg aacgcctacg cgcaattgcg cacgtgggat gcggcggata
      301 tcagtgccaa ttcgctctac gacgacgatc ttgcgcgtgc gctccgggcc attcgggcac
      361 gcgtgctgct gatgcccggg gagactgatc tctatttccg cgtggcggac aatacggccg
      421 agttgcggta tctgtcgcag gcagagttga agccgatacc aagtgtctgg ggccaccgcg
      481 ccggcaatcc aaccacgaac ccggcggacg cccgcttcgt caaggacgct gtgcgggact
      541 ggcttgcgcg atagcataac aaggagaaca gccatggcta tgattaccgc gaacaaccct
      601 tccacagtgc gcgcgccgac cggctacact cactcgatgc tgatcgaagg cgaacagcgg
      661 cgcctgatca tgtcggggca ggttggcatg gcactcgacg gatcggtccc tgccacgggc
      721 gaggggcaga taacgcaggc cttcgccaat cttcgcgcgc tgcttgaggc gaatggcatg
      781 ggtgtcacga atctggtcaa gacgacggtg ttcctgaccg atcgctcgct gctggcctca
      841 ttccgggcgg cgcgcgacgc cgtgtatggc ggtcacgcgc catgctcgac gctgttgttc
      901 gtcgctgggc tggctgaccc gcgctttgtc gtggagatcg aggccgaagc cgtggcgtga
      961 cctcagcccg cgaggcgtgc gtgcgctcca gctatcggga cgagatgcga caggaacgtc
     1021 tcggcgcagg cgcgccagga gtagcgttcc gcgtgcgccc gacatgcggc gcgatccgct
     1081 gtcagggctt cgagcgctgc cgcgcgcaag tcggcgttca ccgcgccgac gctgccatgc
     1141 gcttcggcga gcacgtctat cgggccggtg acgggaaagg ctgccacagg cgtcccgcag
     1201 gcaagggctt cgagcatgac caggccaaat gtgtcggtga ggctggggaa gacgaagaca
     1261 tcagcgccgg cgtaggcacg tgccagtgcc tcgccgtagc gcgggccagt gaagtggacc
     1321 gagggataat cgcgctgcag cttcggcagg atcgggccac cgcccacgac gaccttcgat
     1381 cctggcaggt ccagatcgag gaaggcgccg atgttcttct cgaccgcgac gcggccgaca
     1441 tagatgaaca tcggccgcgg cagtccccag tcctcgcgcg gctcaggctt gaacaggtcc
     1501 agatcgacgc cccgcgacca cgaccggatg ttgcggaaac cacgcttcga cagctcatcg
     1561 cgcagcgatt gcgtggcgac catggtgcct tcgccggcac tgtggaagcg ccgcatccag
     1621 gcatagatcg ggccgaccgg aaggccgata cgtgcttgta tatactcggc gaaccgggtg
     1681 tggaacgcgg tggtgaaatg aaagccgttg cgcatcgccc agctccgcgc ggccatgccg
     1741 agcggtccct cggtggagat gtgcatcgca tccggccgga atgcctcaat catgcgcacc
     1801 atgctccggc gcggcagcaa ggcgagcgga atgtcgctgt aggtcgggca gggaatggtg
     1861 cggaagcggt ccggtgcaat cacctcgaca atgtgcccca tcgcgcgcag ttcgttcacc
     1921 accgtggtga gtgtgcgtac cacgccgttc acctgtggcg accatgcgtc ggtaacgagc
     1981 agaatacggt aggacggcgc gggggagagc atcgcgtcgc ggatggtccg ttcgtgagag
     2041 gtggctgcgt caggcagctt cgcggaccgc ggtgcgacga ggggtgaaga acgacaggcg
     2101 gttcagctcg gcccagtcga taagctcaag ccgtccgtcg tggtgttcga ccagcgcagt
     2161 gcagctttcg acccagtcgc cgtcgttgag atagagcacg ccctggacct cgtgcatctg
     2221 ggcgtggtgg atgtggccgc agacgacgcc gtcgaagcca cggcggcggg cctcggcggc
     2281 cagagccccc tcgaaccggt cgatcgcctt cactgcctcc ttgacctggc gtttcagcca
     2341 tgcggacagc gaccagtagg gataacccag ccgccggcgc actgcgttga agtagcgatt
     2401 gaatgtgagc gcgaatgtgt acgcccagtc gcccagcagc gcgaggaact tggcgtaacg
     2461 cacgacactg tcgaattcat cgccgtgcat gatgagcagt cgcttgccat ctgcggtgat
     2521 gtgttcggct tcctgccgca ggcggatgcc ggcgatttcc attggcagcc acttgcgaaa
     2581 cagctcgtcg tggttgcccg ggatgtaagt cacgtgggtg ccggagcgcg cgaaacggag
     2641 gatcagccgc agcacctcat catggctttc gtcccagtac caggacttgc gcagccgcca
     2701 gccatcgatg atatcgccca ccaggaagag attctggcag gtgatgcggc ggaggaaatc
     2761 ggccaaaaaa tcgcaacgcg cgccacgggt tccgaggtgg gtatccgaaa cgaacacgct
     2821 acggtagagc ggtcgtgcgc cgggtacaat gggtgcgttc atgcgagagc tactggatac
     2881 tctcgaatcc ttgtggataa agttaagttt gaatgacgaa actgaatatt ggcttgatca
     2941 ttgaaagtca tatcttagca acagatttga cattctcttg caacatcggc gatggcagga
     3001 acgcaacttg gcgttgagga gtggtcgatt cgccttggcc atgatcgtcg tgttcaatcc
     3061 ggttgcgggt ttgcggcgtg ccagcctgct ctggcgcgtg ctcgacgtgc tggtggcgaa
     3121 tggcgtgcgc atcgaactgg ccgagacgca tcgggccggc catgccgagg ccctggcgcg
     3181 tgaagcggtc gcgcgcggtg cccagatggt tgtcgccgcc ggaggggacg gcacgatcgc
     3241 cgaggtggcc aacggcctga tgggttcgcc ggcgcgactg ggcgtgatcc cgctaggcac
     3301 ggcgaatgtg atggcgcacg agatggcatt gcccttcgag ccgcgctcgg ttgctgccgc
     3361 cctggcgttc ggacggacgc ggccgctttg gccgggggtt gcgcgaacgg gcgagagttc
     3421 ccggctgttc gttcagatgc ttggcgtggg gctcgatgcg caggtcgtac acaacctgcc
     3481 ggcgccgctg aaacgagcgc tggggcgcag tgcttacgcg ctccagacgc tgcgggagat
     3541 cgcgttctac ccgtaccggc cgatccgcgt tcggctggat ggtgaagaga cgcaggcgac
     3601 gagtgtcatc gtcagtaaag ggcggctgta tggcgggcgc ttcttattgg cgccgacggc
     3661 ttgcgctggc gagccaggct tttcagtggt gctgtttgat cgcgcgggca tgcggtgggc
     3721 gctgacatat ggcgcggctt tgccgttcaa tctgcttgcg catgcgcctg gagttcgcca
     3781 cgtgcgcgct cggtgtatcg agttcatcgg taatgaatcg gtgccggccc aggcagacgg
     3841 cgacgctgcc ggcaggacgc cgatgagcat caccgacgcg ccgtcagcga tcccggttgt
     3901 ggtgggatag gatcaggccg aggtcagtgc ctgatccagg tcggcgatga gatcctcggc
     3961 tgtctccagc ccgatcgaca ggcggatgac atccggtcct gcaccggctg cctggcgttg
     4021 ctcatcggtc agctggcgat gtgtcgtcga agctggatgc agaaccaggc tgcgggtgtc
     4081 accgatattg gcgaggtggg agaacagccc gacattctcg acaagtttca cgccggcctc
     4141 gtagccgccg ctgacgccga aggtgaaaac tgcgccggcg ccgcgcggca gatacttgcg
     4201 cgcgagtgag tgatacgggc tgctttcaag cccggcgtaa gacacccacg cgacgcgcgg
     4261 atcgccatgg aggaattccg ccaccttctg cgcgttggcg cagtggcgtt ccatgcgcac
     4321 atgcagcgtc tcgatgccgg tgatcgtcag gaaggcgttc atcggcgcca tcgtcggccc
     4381 atagtcgcgc agggccaccg cacgagcctt tgtggtgaag gcaaagtctc cgaaattctc
     4441 gtaaaagcgc aggccgtgat aggctggctc cggctcggtg agggcaggga acttgccgct
     4501 tgcggcccag tcgaacttgc cgctctccac taccacgccg cctagcgaat tgccgtggcc
     4561 atcgaggaac ttcgtcgttg aatgcaccac cagatcggcg ccccactcga agggacggca
     4621 cagatacggt gttgccagcg tgttatcgac gatcagcggg atgccagctt cgtgtgcgat
     4681 cttcgcgact gcttcgatgt cgacgatgat gccgccgggg ttcgccagca gttcgaggaa
     4741 aatggccttg cagcgcggtg tgagcgcacg gcggaagttc tcgggatcgg tgggatcgac
     4801 gaaatggcag gtccagccca gcttcttgaa ggacaggccg aactgcgtca gcgatccgcc
     4861 atacaggttg cgcgaggcga ggaactcgtc gccgggttcc atcatcgttg cgaatatgag
     4921 gaactgcgcg gcatggccgg aggcggctgc gcaggcggcg cgaccacctt ccaggctggc
     4981 gacgcgttcc tcgagcacag cgactgtcgg gttggtgagg cgcgagtaga tgtagccgaa
     5041 attatgcaga ttgaataatg acgctgcatg atcgacgtcg tcgaaaacat aagccgcagt
     5101 ctggaagatt ggtgtggtgc gcgcgcccgt tgttggatcg ggcgccgcgc cggcatggac
     5161 cgcgcgggtc tcgaagccgt agccgacatt ggcagatggc tgcgtgcggg gtttcgcctt
     5221 gcgggcggtc ggcgcttgtg gtgctcggtt gttgatgatg cctgagttga cgccgctcca
     5281 ggacagctcg ccgcccaagc ggccgaactc gatcttcggg caacggttca tgatcacttc
     5341 gaggccagcg gcttctgcct ttgccgcggc ttcgtcgttg cggacgccga gctgcatcca
     5401 tacgaccttg gcgccgatgg cgatggcgtc gttgacgatc ggaccgacct gatcggaggc
     5461 gcggaagatg tccaccatgt cgatcttgtc ggggatgtcc cgcaggctgg cgtagatttt
     5521 ttcgccaagc tgttcctggc cggcagtgcc ggggttcacg ggtatgacgc ggtagccttt
     5581 gccctggagg tacttcatga cgaagtagct ggggcggttc cagttggacg acgcgccaac
     5641 catcgcgatg gtccgcacgc tggacaggat gtgacggatc ttcgcgtcgg agtatgggat
     5701 cggatcaact gcggtcatgg gcacctttgc ctataccttt tgccatacgt cggagactga
     5761 catggcgggg cgggaggcgg tggagaagtc tcggcgatgt tgccttgatt cgctcgaggt
     5821 tgcttcgtcg ctttgctcct cgcaatgaca cgaggacacg agaggggcac gagggccgac
     5881 gtcgcggaag ttaccgcgcc tgctggatcg ctgaaaggat ccaacggctg ccgggcgatc
     5941 gcacgaacgt ccacagctcg gtcgcggtga catgctcgtc ggggctgcca tcgacgatgc
     6001 ggccggttct gtcgcgcgtt acgtcgatca tcgagaagcg catggcgacg gtcgcgtact
     6061 cgcggctgcc ctcgctccag gcctgtgcca ggtcgccttg cagcaggcga acgtcggtta
     6121 cttcgttgcg cacgccctgg ctggtctgtt ccgacagctg ctcgctgaag tagctcagca
     6181 tctcgggcgt tgtcgtggcg ctgagagcat tcaggtcgtg gcggctccag gcggcctggg
     6241 tatcacgcag aacctgctcg aaggcctggt agtcctgggg gccgatctgg attggcggcg
     6301 ccgccgccgc ggacccgccg ccgccaccca ttgccgcagg cgctgccgaa cgtgcgaaca
     6361 cgtttgggcc gccggcgaat gccggccggg aagcgccgct gaacatccgg atcagccacc
     6421 ggacgactac gacgatcagc acgatctgaa gcaggaagcc aaggaagcca ccgaagccca
     6481 tcatgccgcc gaagaagcca tggccaagca gcaacccacc gatgcctgcg cctatcagcc
     6541 caccgagcag gccggacatg aagccggagc gcggaccgaa gctggagcca tatgcgccag
     6601 atccgtagcc gggtgatgga ttgttcggcg tgatgctgcg ctgcatgggt gcggcggagt
     6661 aaggcgccgt gcgcgttgca ggtggagcgg accacgtcat gctgccacgg ctgccgaagg
     6721 agccgccgcc gccgagacgg gcatcggcga ggcccggcgc gaagaccacc gcgagcgcca
     6781 caaaagcggc cagcagggtc gaagtgcgac ggaggacgtt tctcgtttgt ttggacatgc
     6841 gcgcaatatg gtgcggcgca gggctatgcg ccagccaggg tcatcccttc aacgcgcact
     6901 gtcggggagt cggtgccgcg gcggaaccgc agatcgttgg ccggtgtcag gtgggcgaac
     6961 atatcgagca gattgccggc gatcgtgatc tcggccaccg gttcggcgag tgctccgttg
     7021 cggatcatga agcccgacgc accgcggctg tagtcgccgg taacgccgtt cacacccatg
     7081 ccgatgagct ccgtgacgta gatgccaagc gtgatgtccg ccatcagttc ggctggagtc
     7141 agggtaccgg cggtcagata gaggttcgtt gtcgagggcg acggcggccc gccggtgccg
     7201 cgcgcggcat gtcctgtgga caccattccg agctggcggg cggagcggct gtcgagcagc
     7261 cacgttgtca atacgccggc agcgatgagg tcgcgtgcgg cggtgggggt gccttcgccg
     7321 tcgaacaggc gcgaccgcag gccgcgtacg cggcgcggat catcgcggat ctcgatatcg
     7381 tcgcggaaaa tgcgcgcgcc gagcttgtcc ttcaggaacg tggtgccgcg cgcgacggag
     7441 gcgccgttga ttgcgccgga caagtggccc agcaggctgc cggcgacccg ctgatcgtat
     7501 acgaccggca gcttcgcggt ggcgggccgg gtcggatgca gccgcgtcac ggcacgctcc
     7561 cccgcgctgc ggccgatgcc ggcggggtcg tccagatcgg ccagatgcac cgtcgaatgg
     7621 tagtcgtagt cgcgctgcat gtccgtgccg gagccggcga gtgcgctggc ggagacggaa
     7681 tggctcgtgc gcgcattctg tccggcaaag ccggccgagg tgacgaggta ggcctcggag
     7741 cggccgtagc cggcttcggc gccctcggag ttcgtgatgc ccggcacagc gagtgccgcc
     7801 tcttccgccg ccgcggcacg cgccacgagg cccttggtcg atggctcggt cgcatcgacc
     7861 agatcaagcg ccagggtgcc tggtggcgcg gccgtgtccg ccagcccggc ataggggtcc
     7921 tcaggcacca cgcgcgccat cgcgacggca cgttcggcca atgcctggaa gctcgacggg
     7981 tctacggtcg tggaggagac gatcg
//
LOCUS       DATGEM010000654         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_587914, whole genome shotgun sequence.
ACCESSION   DATGEM010000654 DATGEM010000000
VERSION     DATGEM010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587914"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..930
                     /locus_tag="VKQ27_20200"
     CDS             181..930
                     /locus_tag="VKQ27_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91318.1"
                     /translation="MIVRDKILPNKAQYQRVEAEIGVPWEMVACLSLRESDCDMRTHL
                     HNGDPLSRRTYHVPAGRPKKGEPPFKWFESAIDALTMPPHSLADVKVWSIERQLYEME
                     RYNGLGYIRHNENSPYVWSWTTEQERGKFVADGRFSGSAWDTQPGCAAILKVLAEAAP
                     EVKARHRVRQATAPPEVVSHATARPRAAAGAGATVATAGGSSKAITQQPDKPMIHHVI
                     EGAMIGLGLLVFIAAAVTVVRRGNLIKAKWG"
     gene            934..1266
                     /locus_tag="VKQ27_20205"
     CDS             934..1266
                     /locus_tag="VKQ27_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91319.1"
                     /translation="MVGEIATVVLTLIVGLVVWDFLHNYTGAPDETKGMLWQRILHGG
                     MGSSTVLVSYLTLIGGWCMTAAGQIGDLVGQPAVHEWLNAHLSAEGMGAVMAGVAVLT
                     FIARLRTL"
     gene            1358..1759
                     /locus_tag="VKQ27_20210"
     CDS             1358..1759
                     /locus_tag="VKQ27_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91320.1"
                     /translation="MLMALIGLIPGALTVIEGIIGLQKDAQVKITMAKLGCDRDTAIA
                     KVNALRDMDHDGVSRLQIIASSKALVFLLLCFALPIAAYDAKVIIWDVMLGWGSTDAI
                     KGVVAEHMNTVMIGIFGAAGAMGAASLWGRK"
     gene            1759..1974
                     /locus_tag="VKQ27_20215"
     CDS             1759..1974
                     /locus_tag="VKQ27_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91321.1"
                     /translation="MGTENYSKGGWIAGATKNKGGLHRSLGVPEGQKIPVGKIEAATH
                     SSNPRVRKQANLAKTLSHMHRGGRRGG"
     gene            1976..2179
                     /locus_tag="VKQ27_20220"
     CDS             1976..2179
                     /locus_tag="VKQ27_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91322.1"
                     /translation="MNTNPTEFWAKVNDPENVRKLNELAMSPEGAHTTPGHGAPRCPT
                     CDGLIGKCDHMSHYPENYGEINI"
     gene            2197..>2612
                     /locus_tag="VKQ27_20225"
     CDS             2197..>2612
                     /locus_tag="VKQ27_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91323.1"
                     /translation="MAEKIAANHVLQAQRPRVDLQEAPAAEAAAPEAEDKFLDIPDDA
                     SPTETVREYGGMRVISNKEGFRVERSSSPRQVTPPPPPPPPLSERTLREMEAGRKVLE
                     RFHPRPAAAVDPTKTVPTGIPTAGVAEYGGARGLKAP"
BASE COUNT          510 a          817 c          863 g          422 t
ORIGIN      
        1 acgtctttca acgtgaacat tcagatcacg accaacctga gccaggtgaa gaacgaccac
       61 gtcgcattct cgatcgtggc cgcatagggg ggtgccatgg gctatcaatc cagcgcggcg
      121 ggttacgcca acctgtggga ccactgccgc gtgcgcacgg agaaaaccgg cgccgctgcg
      181 gtgatcgtcc gcgacaagat cctgcccaac aaggcgcagt atcagcgcgt cgaggccgag
      241 atcggcgtcc cctgggagat ggtcgcatgc ctgtcgctcc gcgagagcga ttgcgatatg
      301 cgcacccacc tgcacaacgg tgacccgctg agccggcgca cctatcacgt tcccgcgggc
      361 cgccccaaga agggcgagcc gccgttcaag tggttcgaga gcgcgatcga cgcgctcacc
      421 atgccgccgc acagcctcgc cgacgtcaag gtgtggtcga tcgagcggca gctctacgag
      481 atggagcgct acaatggcct cggctatatc cgccacaacg aaaattctcc ctacgtatgg
      541 tcgtggacga ctgagcaaga gaggggcaag tttgttgctg atggccgctt tagcggtagc
      601 gcttgggaca cgcagcctgg gtgcgcggcg atcctcaagg ttcttgcgga agcagccccc
      661 gaagtgaagg cgcgccatcg cgtgcgccag gccactgcgc cgcccgaagt cgtctcgcac
      721 gcgaccgcgc gaccgcgcgc ggctgccggc gccggcgcca ccgtcgcaac ggccgggggt
      781 agctccaaag caatcacgca gcagcctgac aagccgatga ttcatcacgt catcgagggt
      841 gccatgatcg ggctcggact gctcgtgttc atcgccgctg ccgtgacagt cgtccggcgc
      901 ggcaatctca tcaaggcaaa atgggggtaa gtcatggtcg gtgaaatcgc tactgtcgtc
      961 ttgacactca tcgttgggct cgtcgtgtgg gacttcctgc acaattacac gggcgcgccg
     1021 gacgaaacca agggcatgct gtggcaacgc atcttgcacg gcggcatggg gagctcgacc
     1081 gtcctcgtgt cgtacctcac gctgatcggc gggtggtgca tgaccgctgc cggtcagatc
     1141 ggagacctgg tcggacagcc cgcagtgcac gagtggttga acgcgcacct gtccgctgag
     1201 ggcatgggcg cggtaatggc cggtgttgcc gtgcttacgt tcatcgcgcg cctgcggacg
     1261 ctctagttat gggctgggtc attttcggcg tgatactggc gctcgtgctg ctggcggtta
     1321 tcgacgtcgc cgggtccggt ggcggggagg ggtaacaatg ctcatggcgc tgatcgggct
     1381 catacccggt gcgttgaccg tcatcgaggg gatcattggc ctgcagaagg acgcgcaggt
     1441 caagatcaca atggcgaagc ttggctgcga ccgggacacc gcgatcgcca aggtcaacgc
     1501 gctgcgcgac atggaccacg atggcgtcag ccggctgcag atcatcgcca gcagcaaggc
     1561 gctggtgttc ctgctcctgt gtttcgccct gccgatcgcc gcctatgacg ccaaggtgat
     1621 catctgggac gtgatgttgg ggtggggctc gaccgacgct atcaagggcg tcgtcgctga
     1681 gcacatgaac accgtcatga tcggcatctt tggcgcggcc ggcgcgatgg gcgcagcctc
     1741 gctatggggg aggaaatgat gggtacagag aactacagca aaggcggctg gatcgccggc
     1801 gccaccaaga acaagggtgg cctgcaccgc agcctcggcg tgcctgaagg gcagaagatc
     1861 ccggtcggca agatcgaggc ggcgacacat tcatcgaacc cgcgcgtgcg caagcaggca
     1921 aacttggcga agacgctgag ccacatgcac cgcggcggaa ggcgaggagg ttgacatgaa
     1981 cacgaaccca accgagttct gggccaaggt caacgacccc gagaacgtgc gcaagctgaa
     2041 cgagctggcc atgtcgccgg agggcgccca cacgacgcca ggtcacggcg cgccgcggtg
     2101 tccgacttgc gatggtctga tcggcaagtg cgaccacatg agccactatc cggaaaatta
     2161 tggggagata aacatatgag catcccgaca caaggcatgg cggagaagat cgccgccaat
     2221 cacgttctgc aggcgcagcg cccgcgcgtc gacctgcaag aagcacccgc tgcagaagcc
     2281 gccgccccgg aggccgagga caagttcctt gacatcccgg acgacgcgtc gccgaccgaa
     2341 accgttcggg agtatggcgg catgcgcgtt atctccaaca aggagggctt ccgggtcgag
     2401 cgcagcagtt cgccgcgtca agtcacgccg cctccgcccc cgccgcctcc gctctctgag
     2461 cgcacgctgc gcgagatgga agctggccgc aaggtgctcg agcgcttcca cccgcgcccc
     2521 gctgccgcgg tcgatccaac caagacggtg ccaactggaa taccaacagc gggcgttgcc
     2581 gagtatggcg gcgcgcgcgg gctaaaggca cc
//
LOCUS       DATGEM010000655         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1656581, whole genome shotgun sequence.
ACCESSION   DATGEM010000655 DATGEM010000000
VERSION     DATGEM010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656581"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VKQ27_20230"
     CDS             <1..1231
                     /locus_tag="VKQ27_20230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HLY91324.1"
                     /translation="SGTVIEARIGGVRVGELCQLRDPAGATAEAEVVGVAGDRAMLVP
                     IGAIDGLSTRAELEPTGRQLVVPVGEALLGRMLDGLGRVLDRPDVPLTGCELISIDAS
                     PPSALGRRPVERVLPLGTRAVDALLTCAEGQRIGIFGPAGTGKSTLVARIVQGADADV
                     FVVALVGERGREVGEFVQRTLRPEARRRSVIVAATSDRPALERVKAAQVATAIAEWFR
                     DRGKRVVLLVDSLTRLARAWREIGLAAGEPPTRRGFPPSVFAALPRLLERAAPNAAGT
                     ITAFYTVLIEGELAADPIAEEVKSILDGHLILSPRLSEADHHPAIDILASRSRVMAHV
                     VDETHGAAATHLRRLLSRHQEIELLVRVGEYQAGSDPLADEALRKIGPIREFLREPGS
                     EITPFATTLQRLRALAA"
     gene            1263..2546
                     /locus_tag="VKQ27_20235"
     CDS             1263..2546
                     /locus_tag="VKQ27_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91325.1"
                     /translation="MLIDEVDAAASTVEHANAGENFAAALDDAKTTHPDTSPPSHPGH
                     SGGGGILGGLWHDAVSAVSGVSSAYNTVNGAMHSAGHALDNVAQSGISLGTGLVRDIA
                     GNGAANVFHGAAEGLRQSAKDTAGFAYGLGEGGIEALGGMAKGVGDLVGDSYRFATDG
                     NFRGSVVHGAESVVTHVAQDPIGSAKALSSGLLHAGENWVRGAGQAAVSGDLGEYVGK
                     GAGNLAVNVGGFFIPGADAADAVSLAGKAGEAAEGLNLASKAGDAANALGDAEKAGEL
                     GNAGRAGETTEASGTIDLGRTNADPLKPAADPLAEPVRSAKTFSSFEEFEAASNHPAP
                     NTSYRLGDYEWSTDAQGRTIEAGGTVKVQEFGRNNPSLQQEIGHEGRSTDVGFHLIAD
                     SLDGPTVRLNGVFDESSQGRFDKYLDTYRAERAAL"
     gene            2574..3383
                     /locus_tag="VKQ27_20240"
     CDS             2574..3383
                     /locus_tag="VKQ27_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91326.1"
                     /translation="MSGSVSSNQPTPAHDVAKIHGLLNNDGFLGHDENDHLHQVSKLL
                     SGLNSSDLHRVMTGLSQGDLKKFGSEKDDGGTPVFGGLIGNNGLSSGERQSVFNQLAA
                     HSTSGADLSRLSGDLSSRDDIQALGNAIAAHARDATKVGYISGIAPSTTQQNDANKFG
                     FGYSTSTPGSSQANAVGSVPASLKGHSVDQALGGPDDQQLGVVMKAAEGETLTTTSDG
                     FGAATTVSYDTTKLAAINSAAASGSDPGQKARVFEADAAPGADQGRSVMQA"
     gene            3380..3868
                     /locus_tag="VKQ27_20245"
     CDS             3380..3868
                     /locus_tag="VKQ27_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91327.1"
                     /translation="MTRDELQTLVSLKRAREQRAMAALTRCRAAEAQAVAARQQTGRA
                     VSHHAAHRLAQESAIYARLLAEPLPVRRLQSAAAQLARMAAYDAVLRRQLADASRVLA
                     RRVEDRSAAALVRSRAEQRAAAMELLADEWHAESAAAEERCAEYEIEETTEDARRSVK
                     AR"
     gene            3865..>4737
                     /locus_tag="VKQ27_20250"
     CDS             3865..>4737
                     /locus_tag="VKQ27_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91328.1"
                     /translation="MNAIAGALIAVPYHPPALSQVEAVIARCFAARRRPIPLGDGELS
                     LLPPPAQVSGAMAVGLTFGTGPARCILPRPCIDRILEALDPAASKAEPDVQALLLELA
                     IEPWLAQLDAVLPVTLGADPGELPLAALLGLRWRDGASSDDVARLELGPAAAAALAGV
                     ITAMPLQRRRIDGLVVPLQVRALTAELTLAELREIENGDVILADAPAEGGTLLVAGER
                     LVWRARLDQHRLRVVTTRQFVGACGLQEWTMQDPEQPAAPDVSLDELPLRLTFELGRL
                     EVPLAELETVGPGYV"
BASE COUNT          807 a         1561 c         1638 g          731 t
ORIGIN      
        1 ctcgggcacc gtgatcgaag cccggattgg cggcgtgcgc gtcggcgaac tctgccagct
       61 gcgtgatcct gccggcgcca cggccgaggc agaggtcgtc ggtgtggccg gagaccgcgc
      121 catgcttgtg ccgataggtg ccatcgacgg cctctcgacc cgcgccgaac tggaaccgac
      181 cgggcggcag ctcgtggtgc ccgtgggcga ggctttgctg gggcgcatgc tggacgggct
      241 gggccgcgta ctggaccggc ctgatgtgcc tctgaccggc tgcgagctga tcagcatcga
      301 tgcgtcgccg ccatccgctc tgggccgccg gccggtggag cgggtcctgc cgctcggcac
      361 ccgggcagtc gatgcgctgc tgacctgcgc cgagggccag cgcatcggca ttttcggtcc
      421 tgccggcacc ggcaagtcca cgctggttgc gcgcatcgtg cagggcgccg atgccgacgt
      481 gttcgtcgtc gccctggtgg gcgagcgcgg gcgcgaggtt ggcgaattcg tgcagcgtac
      541 gctcaggccg gaggcgcgtc ggcgctcggt catcgtcgcc gccacctccg atcgccccgc
      601 tttggagcgc gtgaaggctg cgcaggtcgc gaccgccatc gccgagtggt tccgcgaccg
      661 cggcaagcgt gtggtgctgc tggtggacag cctcacccgc ctggcccgcg cctggcgtga
      721 gattggcctg gcggccggag agccacctac gcggcgcggc ttcccgccct cggtcttcgc
      781 cgccttgccg cgcctgctgg agcgcgccgc gcccaacgcc gcgggcacca tcaccgcttt
      841 ctacacggtg ctgatcgaag gcgagctcgc cgctgatccg attgccgagg aggttaagtc
      901 gatcctggat ggccatctga tcctgtcgcc acgcctgtcg gaagccgatc atcatccggc
      961 gatcgacatc cttgcgagcc gcagccgcgt catggcgcac gtcgtagacg aaacgcatgg
     1021 cgccgcggcg acgcatcttc gccgcctgct gtcgcggcat caggagatcg agctgctggt
     1081 gcgcgtcggc gaataccagg ccggcagcga tccactggcg gacgaagcgc tgcgcaagat
     1141 cggaccgatc cgagagttcc tgcgtgagcc gggcagcgag atcacgccct tcgcgaccac
     1201 gctgcagcgc ctacgggccc tcgcagcatg aaccgcatca cagaagcccg aggagcgacg
     1261 caatgctgat cgacgaagtc gatgccgcgg caagcaccgt cgagcacgcg aacgctgggg
     1321 agaatttcgc tgctgcgctc gatgatgcaa agaccacgca tccggatacg tcgcccccct
     1381 cgcatcctgg tcacagcggc ggcggtggca ttcttggcgg tctgtggcac gacgccgtca
     1441 gcgccgtcag cggcgtcagc agcgcctaca ataccgtgaa cggcgccatg cactccgccg
     1501 gtcatgcgct cgacaatgtg gcgcagtcag gcataagcct gggcactggc ctggtgcgcg
     1561 acatcgccgg caacggcgcg gcgaacgtgt tccacggcgc cgccgaaggc ttgcggcagt
     1621 ccgcgaagga cacggccggc ttcgcctatg gactcggcga gggcggcatc gaagcgctcg
     1681 gcggcatggc caagggtgtt ggcgacctcg tcggcgacag ctatcggttc gccacggacg
     1741 gcaacttccg cggctcggtg gtgcatggtg cggagagcgt tgtcacgcac gttgcgcagg
     1801 atcccatcgg cagcgccaag gcgctcagct ccggcctcct gcatgctggc gagaactggg
     1861 tgcgcggcgc cggccaggcc gctgtcagcg gcgacctggg cgagtatgtc ggcaagggcg
     1921 ccggtaatct cgcggtcaac gttggcggct ttttcatacc gggtgcggat gctgccgatg
     1981 ccgtcagtct ggcgggcaag gccggggaag ccgccgaggg actgaacctc gcgtccaaag
     2041 cgggggacgc agcgaacgct ctgggcgatg cggagaaggc aggagaactc ggcaatgcgg
     2101 ggcgcgccgg cgagacgacg gaagcctccg gcacgatcga tctggggcgg acaaatgcgg
     2161 atccgctgaa acctgccgcg gatccactgg cggagccggt gcggtcggca aagacgttct
     2221 caagcttcga agagttcgag gctgcatcga atcacccggc gcctaacacc agctatcgcc
     2281 tcggcgacta cgagtggtcc accgatgcgc aaggccgcac catcgaggca ggcggcacgg
     2341 ttaaggtgca ggaattcggc cgcaacaacc cgtcgctcca gcaggagatc ggccacgagg
     2401 gccgctccac cgatgtcggc ttccatctga tcgctgactc gctcgatgga ccgacggtgc
     2461 ggctgaacgg cgtgttcgac gaatcctcac aaggccgctt cgacaaatac ctcgacacct
     2521 atcgggccga acgcgcggca ctctagcccc gcaaacagca gaagggatcc gcaatgtcag
     2581 gcagcgtcag cagcaaccag ccaactccgg cacatgacgt tgcgaaaata catgggctgc
     2641 tcaacaacga cggcttcctg gggcatgacg agaacgacca tctgcatcag gtctcgaaac
     2701 tgctgtccgg cctgaacagc agcgacctgc accgggtcat gacggggttg agccagggcg
     2761 atctgaagaa gttcggcagc gagaaggatg atggcggcac gccggtgttc ggcggcctga
     2821 tcggcaataa cggcctgtcg tcgggcgagc gtcagagcgt gttcaaccag ctcgccgcac
     2881 acagcaccag tggcgccgac ctctcgcgtc tatccggcga tctgtccagc cgcgacgata
     2941 tacaagcgct gggcaacgcc attgctgctc atgcgaggga cgccaccaag gtcggctaca
     3001 tcagcggcat agcgccatcc accacacagc agaacgacgc caacaaattc ggcttcggct
     3061 actcgacgtc gactcccgga agctctcagg ccaacgcggt gggctcggtt ccggccagcc
     3121 tgaagggaca ctccgtcgac caggcgcttg gcggcccgga tgatcagcag cttggcgtgg
     3181 tgatgaaggc agcggagggt gaaacactca caaccacctc cgatgggttc ggcgccgcta
     3241 caacggtgag ctacgataca acgaagctcg ctgccatcaa cagcgccgcc gcgtccggca
     3301 gcgatcccgg acagaaggcg cgcgtattcg aagccgacgc ggctcctggt gctgaccaag
     3361 gcagatcggt gatgcaggcg tgacgcggga cgaactgcaa accctggtat cgctgaagcg
     3421 tgcccgcgag caacgggcca tggcggcact gacgcgttgc agagcagcag aggcacaggc
     3481 ggtagcggcg cgccagcaga ccggccgggc cgtgtcacat cacgctgcac accggctcgc
     3541 ccaggagtcg gcgatctacg caaggctgct ggcggagccg ttgccggtgc ggcgactgca
     3601 atctgccgcg gcgcaactag cgcgcatggc cgcctatgat gccgtgctgc gtcggcagct
     3661 tgccgatgca tcgcgcgtgt tggcgcggcg cgtggaagat cgctcggccg ccgcgctcgt
     3721 gcgatcgcgc gccgagcagc gggcggctgc gatggaactg ctcgccgacg agtggcacgc
     3781 cgaaagcgcc gcggccgagg aacgctgcgc tgaatacgaa atcgaggaga cgacggagga
     3841 tgcgcggcgc tccgtcaaag cgcgatgaac gcgatcgccg gggcgctgat cgctgtgccg
     3901 tatcatccgc ctgcgctgtc gcaggtcgag gcggtcattg cccgctgctt cgccgcacgg
     3961 cggcggccca tccctctcgg cgatggcgaa ctcagtctcc tgccaccacc ggcgcaggtg
     4021 tcgggcgcga tggctgttgg cctgacgttt ggtaccgggc cggcgcgctg catcctgccg
     4081 cgtccatgca tcgatcggat actggaggca ctcgatccgg ccgcttccaa ggccgaacca
     4141 gacgtccagg cgctgctgct ggaactcgcg atcgagccgt ggctggcgca actggacgca
     4201 gtgctgcctg tgacgctggg cgctgatccc ggcgaattgc cattggccgc gctgttgggt
     4261 ctgcgctggc gcgacggcgc gagcagcgac gatgtcgccc ggctggaact ggggccggcc
     4321 gcagcagccg ccctagccgg cgtgatcaca gcgatgccgc ttcagcgccg gcgcatcgac
     4381 gggcttgtcg ttccgctcca ggtgcgtgcg ctgacggccg aactgacact cgccgaactt
     4441 cgcgaaatcg agaacgggga cgtgatcctc gccgatgcgc cggccgaggg aggtacgctc
     4501 ctggtcgccg gcgagcgact ggtctggcgt gcgcgtctcg atcagcaccg gctgcgtgtc
     4561 gtcacgacgc gccaattcgt cggtgcatgc ggattgcagg agtggacgat gcaggatccc
     4621 gaacaaccgg cagcacccga cgtgtcactg gatgaattgc cattgcgtct caccttcgag
     4681 ctgggccggc tagaggtgcc gctcgctgaa ctggaaaccg tcggacctgg ctacgtg
//
LOCUS       DATGEM010000656         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_390720, whole genome shotgun sequence.
ACCESSION   DATGEM010000656 DATGEM010000000
VERSION     DATGEM010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390720"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VKQ27_20255"
     CDS             complement(<1..818)
                     /locus_tag="VKQ27_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY91329.1"
                     /translation="MAEPVITPLDWADPFNLSEQLTEEERLVRDTAYGYAQEKLLPRV
                     TSAYLDERFDREIMTEMGALGLLGATIPHEYGGAGLGYVAYGLIAREVERVDSGYRSA
                     MSVQSSLVMHPIYAYGSEEQRKKYLPKLATGEWVGCFGLTEPDAGSDPAGMRTRATKI
                     DGGYRLDGAKMWITNSPIADLAVVWAKSDAHDGAIRGFIVERGAKGFSTPKIEGKLSL
                     RASITGEIVLDGVEVGEDALLPNVQGLKGPFGCLNRARYGISWGAMGAAEDCMHR"
     gene            complement(878..1195)
                     /locus_tag="VKQ27_20260"
     CDS             complement(878..1195)
                     /locus_tag="VKQ27_20260"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLY91330.1"
                     /translation="MATAGQERRAEFRREPDTDTMVRAYRLPPVPATLRDISTGGAML
                     KVNGAAFDIGDEVVLICGDIEIVATIAWRKSGFFGLAFHRRLDHREMRALRVTHPLEQ
                     HGL"
     gene            1358..2542
                     /locus_tag="VKQ27_20265"
     CDS             1358..2542
                     /locus_tag="VKQ27_20265"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HLY91331.1"
                     /translation="MPWKEVSVMDERLRFIARVLDGEPMTDVCREFGISRKTGYKLFN
                     RYRDEGPVALCDRSRRPVRYANQLPEQVERLLVATKKEKPHWGARKIRELLIRKLDGD
                     YRIPSVSTVHSVLDRHGLVTHAIRRRAKASGTALSPGLVPNELWCTDFKGEFKLGNGK
                     YCYPLTVTDHASRYLLTCEALDSNREMPVIETFERLFKDRGLPAAIRSDNGVPFASPN
                     GLYTLSKLSVWWLRLGISIERIKPGHPQQNGRHERMHLTLKREATRPAGGNSLQQQAR
                     FDSFVQEFNNERPHEALAMKCPAELYAPSAKPYQGLPDVEYPFHDREVLVTACGRICM
                     YRKRINISTVLAGQKLGIKEVDDGIWLVSFLAYDLGYIDLEQKTLQTLDNPFGPRLLP
                     MS"
     gene            complement(2587..2676)
                     /locus_tag="VKQ27_20270"
     tRNA            complement(2587..2676)
                     /locus_tag="VKQ27_20270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2640..2642),aa:Ser,seq:tga)
     gene            2916..3902
                     /locus_tag="VKQ27_20275"
     CDS             2916..3902
                     /locus_tag="VKQ27_20275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HLY91332.1"
                     /translation="MADVPADPHDAPFRSYLQSFRSRALAQGVSAQTLDSVLPTLIYN
                     PRVVELDQAQPGGSPTGPASAVSAFAPYRAKHVGADLITRGRAAYAAQRFNLQRVEAE
                     TGVPESVMIAIWGQETSYGSYTGDFDLARSLATLAFEGRRRDLFESELIATLKLIDRG
                     IPRAQLKGSWAGATGYPQFLPSVYLRVGRDGDGDGRADIWTNPADALASIGSYLVNAG
                     WKAGEPWGVPAYVPATLDRTAIGTRMTAPRCPRVHSRHSRWLTMREWRALGVVQMGGP
                     MVSDSEQATLLEPDGPGATAYLTFGNYRAILDYNCSNFYALSVGLLADAVVN"
     gene            3886..>4379
                     /locus_tag="VKQ27_20280"
     CDS             3886..>4379
                     /locus_tag="VKQ27_20280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLY91333.1"
                     /translation="MPSSIEGRTRRFGGLAGLLLLAGCGGGVHGLVPDTPVKLGRPYM
                     VGGRSYVPVDNPAYDQIGEASWYGNAEQGRSTANGETFYRQRPSAAHTTLPLPSYVEV
                     TRLDTGQRILVRVNDRGPYARDRILDLSEEAARELGIQRAGRALVRVRRVTPGSSERK
                     RLQRG"
BASE COUNT          798 a         1378 c         1388 g          815 t
ORIGIN      
        1 cggtgcatgc aatcttcggc cgcgcccatc gcgccccatg aaatgccgta gcgcgcgcga
       61 ttgaggcagc cgaacggccc cttcagcccc tggacgttgg gaagcagggc gtcctcgccg
      121 acttcgacgc cgtcgagcac gatctcgccg gtgatcgatg cacgcagcga aagcttgcct
      181 tcgatcttgg gagtcgagaa acccttggcg ccgcgctcga cgatgaagcc ccggatcgca
      241 ccgtcatgcg cgtccgactt ggcccacacc actgcgagat ctgcgatcgg cgagttcgtg
      301 atccacatct tggcaccgtc gaggcgatag ccgccgtcga tcttggtcgc gcgggtgcgc
      361 atgccggccg gatcggatcc ggcatcgggc tcggtcaggc cgaagcagcc aacccactcg
      421 ccggttgcga gcttggggag atacttcttc cgctgctcct cggagccgta ggcgtagatc
      481 gggtgcatga cgagcgagga ctgcaccgac atcgccgagc gatagccgct gtccacgcgc
      541 tcgacctcgc gcgcgatcag gccataggcg acataaccca gccccgcgcc gccatattca
      601 tgcgggatgg tggcgccgag caggccgagt gcccccatct cggtcatgat ctcgcgatcg
      661 aaccgttcgt cgagataggc cgaggtgacg cgcgggagca gcttctcttg cgcatagccg
      721 tacgcagtat cgcgaacgag gcgttcttcc tcggtcagct gctcggaaag gttgaagggg
      781 tccgcccaat cgagcggcgt gatcacgggt tcggccatgg catcctctac ttggttgccc
      841 taggccaaag cctgttccgt cctgcttggc aaccccctca gaggccgtgt tgctccagcg
      901 gatgcgtcac gcgtagcgcg cgcatctccc ggtgatcgag ccggcggtgg aaagcgaggc
      961 cgaagaatcc gcttttgcgc caggcgatcg tggcgacgat ttcaatgtca ccgcagatga
     1021 gaacgacttc gtcaccaatg tcgaaggcgg ctccattaac cttcagcatg gcaccgccgg
     1081 tcgatatgtc gcgcagcgtg gcagggaccg gaggcaggcg ataggcgcgc accatcgtgt
     1141 cggtgtcggg ctcgcgccga aactctgcgc gccgttcttg gccagcagtg gccattgccg
     1201 tcgcactccc ctccggatcg ttactcgttg ggtaacattg tatcgttgaa acatttaacg
     1261 atctgtcaat caggtgagcg ttcggagaga taggtgacgg actgttccgg agacatgggt
     1321 gacacgtctc cccagcgatc gctggggaga cgtgtcgatg ccgtggaagg aagtgtccgt
     1381 gatggacgaa cgtctacgct tcattgcccg cgttctcgac ggcgagccga tgaccgacgt
     1441 gtgtcgggag ttcgggatat cccgcaaaac cgggtacaag cttttcaacc gatatcgcga
     1501 cgaagggccg gtcgctttgt gcgaccggtc gcggcgcccc gtacgatacg ccaatcagtt
     1561 gcccgagcag gtcgaacgct tgctggtcgc aacgaagaaa gagaagcccc attggggggc
     1621 tcgcaagatt cgcgaacttt tgatccgcaa gctcgatggc gactatcgga tcccctcggt
     1681 cagtaccgtc catagcgtcc tggatcgtca tggtctggtg acccacgcca taaggcgacg
     1741 cgcaaaggcg tcgggaaccg ccctgtcgcc aggccttgtc cccaatgagt tgtggtgcac
     1801 ggacttcaag ggcgagttca aactgggcaa cgggaaatac tgctatccgt tgaccgtgac
     1861 cgatcatgcc tcgcgctatc tgctcacctg tgaagcgctg gattcaaacc gggaaatgcc
     1921 ggtcatcgaa accttcgaac ggctgttcaa agaccgcggc ctcccggctg ccatccgctc
     1981 cgataatggc gtcccctttg ccagccccaa tggcctctac accctctcca aactctcggt
     2041 ctggtggctg cgcctgggaa tctcgatcga acgcatcaag cctggccatc cccagcaaaa
     2101 cggccgccat gaacgcatgc acttgaccct caagcgcgag gcgacccggc cagcgggcgg
     2161 gaatagcctc cagcaacagg cccgcttcga cagctttgtc caggagttca acaacgaacg
     2221 cccccacgaa gcgctcgcca tgaaatgccc cgctgagctc tatgcgcctt cggccaagcc
     2281 ctatcagggc ctgcccgacg tcgaataccc cttccacgac cgcgaagtcc tcgtcaccgc
     2341 ctgcggtcgc atctgcatgt accgaaagcg gatcaacatc tcgaccgtgc tcgccgggca
     2401 aaagctcggc atcaaggaag tcgacgacgg catctggctc gtctccttcc tcgcctacga
     2461 tctaggctac atcgatctgg agcagaaaac tttgcaaacc ctcgacaacc cgttcggccc
     2521 gaggttgtta cccatgtcat aggtacagat cgtcaccgat ctctccggta tggacaaagg
     2581 ctgcattggc gggcggggtg ggattcgaac ccacggtgag cgtgaaccca cggcggtttt
     2641 caagaccgct gccttaaacc actcggccac ccgcccggat gacgcctgga tcgtgagcgc
     2701 ccgcttatcg cggctgcgcc ccaatgtgca agcaggacga cgagacgggc tgacctcctc
     2761 gtccgcctat gccaaaagcg gcgaacggag gtggggcatt gcaggtagcg tttcgtatgt
     2821 tcggcttggc ggggatcctc gcactggggg ccgtcgcacc gtcttatgga cccggccatg
     2881 gatcggcagc atccgctcag gaatggaccg ggcaggtggc tgacgtgccg gccgatccgc
     2941 acgatgcgcc attccgcagc tatctccagt cgtttcgtag ccgggccttg gcgcaggggg
     3001 tgtcggcgca gacgctggat agcgtcctgc caacgctgat ctacaacccg cgcgtcgttg
     3061 agttggatca ggcccagccc ggcggctcgc cgaccggccc agcttccgcg gtttctgctt
     3121 tcgccccgta tcgagcgaag catgtcggag ccgacctgat cacacggggg cgggcggcct
     3181 atgcagcgca acgcttcaac cttcagcggg tcgaagccga aacgggtgtg cccgaatcgg
     3241 tgatgatcgc gatctggggg caggagacga gctatggcag ctatacaggg gatttcgatc
     3301 tcgcgcggtc gttggcgaca ttggcgttcg agggacgccg ccgcgatctg ttcgaaagcg
     3361 agctgatcgc aaccctgaag ttgatcgacc gcggcatccc ccgggcgcag ctgaaaggca
     3421 gttgggcagg ggcgaccggc tatccccaat ttcttccctc agtctatctt cgagtggggc
     3481 gcgacggtga cggagacggc cgcgcggaca tctggaccaa ccccgccgat gcgctcgcct
     3541 cgattgggag ctacctcgtt aacgcggggt ggaaagccgg cgaaccttgg ggcgtgccgg
     3601 catatgtgcc ggcaacgctg gacaggaccg caatcggtac gcggatgacc gcgccccgct
     3661 gcccgcgtgt tcattctcgc cacagtcgct ggctgaccat gcgcgaatgg cgagcgctgg
     3721 gtgtcgtgca gatgggcgga ccgatggtat ccgacagcga gcaggcgacc ctgctggagc
     3781 cggacggtcc cggtgccacc gcctatctga ccttcggtaa ttatcgggca atcctcgatt
     3841 ataattgttc gaacttttac gcgctgtccg tgggattgct ggccgatgcc gtcgtcaatt
     3901 gagggccgca cgcggcgatt cggcgggttg gcggggctgt tgctgctcgc cggctgcgga
     3961 ggtggcgtgc atggtctcgt acccgacacg ccggtgaaac tggggcggcc ttacatggtc
     4021 ggcggccgga gttatgtccc tgtcgacaat ccagcttacg atcagatcgg tgaagctagc
     4081 tggtacggca atgccgagca gggccggtcg accgcgaatg gcgaaacctt ctatcgtcaa
     4141 cggccgagtg ccgcgcatac gaccctgcct ttgccatcct atgtcgaggt gacgcggctc
     4201 gataccggcc agcgtattct ggtgcgggtc aatgatcgcg ggccttatgc gcgggatcgc
     4261 atcctcgatc tgtcggagga agcggcgcgg gagctcggca tccagcgggc gggccgtgcg
     4321 ctggttcgtg tccgccgggt gacaccgggc agcagcgagc ggaagcgatt gcaacgcgg
//
LOCUS       DATGEM010000657         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1533440, whole genome shotgun sequence.
ACCESSION   DATGEM010000657 DATGEM010000000
VERSION     DATGEM010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533440"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VKQ27_20285"
     CDS             <1..405
                     /locus_tag="VKQ27_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91334.1"
                     /translation="AILIGGSGLYLMLVTHALGGGSPLGTAVLTIAFYVVVVSPWGIV
                     STYISERFPTQVRASGYGIGYSAAVVIPAFAGFYMLGLSSFMPYVFTPLVLLLAAGLL
                     ILVGVLMGPETRDVELHAADLGQGGLTQGYAD"
     gene            complement(428..586)
                     /locus_tag="VKQ27_20290"
     CDS             complement(428..586)
                     /locus_tag="VKQ27_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91335.1"
                     /translation="MPTAKQLREWAATIRTMLAEIDNISVAERAAPLIAELETLAACE
                     EAVERQLV"
     gene            complement(891..1331)
                     /locus_tag="VKQ27_20295"
     CDS             complement(891..1331)
                     /locus_tag="VKQ27_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLY91336.1"
                     /translation="MRKGSVVVHYGKCFCGVVALEVSGTPEAMGYCHCNSCRSWSGGP
                     VNAFSLWKPEAVRITSGAEFIATFQKTPLSQRKFCTKCGGHLMTKHPPLGLIDVFTAT
                     LPTLTFTPDVHVNYAETVLPMRDGLPKLRDFPAAFGGSGEVIAG"
     gene            complement(1397..>2478)
                     /locus_tag="VKQ27_20300"
     CDS             complement(1397..>2478)
                     /locus_tag="VKQ27_20300"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLY91337.1"
                     /translation="DGDEAGADHLNRALAMAREEGWDALAGLAYLNIGSSYGEVYLFP
                     EAERYLAEGIDYARERDLDHATHYMTAWLALVRLYQGRWTEALELAEWVVGRPGTSAI
                     TRIMALIALGRLRTRRGEPGAEQVLDEALVLALQTDTLQRLAPVRSARAEAAWFAGDA
                     PRVIAEATATYDLAVRSAHCWHAGEFAFWRHLCGDAVSAPSWSASPFTLQMSGDWQRA
                     AEQWARLGCPYEQARALADGDTDAKLAALEIFDRLGAAPAAAVLRQRMRGEGMSRIPR
                     GPRATTRRNRFGLTVREMEILACVAIGLSNGRIGARLHVSPKTVDHHVSSVLAKLGAT
                     TRIEAAEIAREQNLLPQNGEIISAK"
BASE COUNT          435 a          796 c          777 g          470 t
ORIGIN      
        1 gccatcctaa ttggcggcag cggcttgtac ttaatgctgg tcacccacgc gctcggcggc
       61 ggatcgccgc tcggcacggc agtactgact attgccttct atgtggtggt cgtatcgcca
      121 tggggcattg tcagcacgta catttccgag cggttcccta cgcaagtccg cgcgagtggc
      181 tacggcatcg gctacagcgc ggcggtggtg atccctgcgt ttgccggctt ctacatgctc
      241 ggcctgtcga gcttcatgcc gtatgtgttc acgccgcttg tactgctgct cgcggcgggg
      301 ttgctgattt tggtcggggt gctgatggga ccggagactc gcgacgtgga gctgcacgcc
      361 gccgatctcg ggcagggcgg gctaacccag ggttacgctg actgaacgcc gggggcactg
      421 gaagccgcta tacaagctgc cgttccactg cttcctcgca ggcagccagg gtttccagtt
      481 ccgcaatcag cggagccgcg cgttctgcca cgctgatgtt gtcaatctcc gccagcatgg
      541 tccgaatcgt ggcggcccat tcgcgcagtt gcttggctgt aggcattgtt ctctcctttc
      601 gaatgcgcgg cgaacagcga cgcgatccag gcggcgaagc ccctggcgcg gtgctgccga
      661 gcgacctcaa gtacatgcgc gctgtcggtg ccggcagctc tcgtcgcaca gtgcagcgcc
      721 agcagcgccg cctgtgtcgt cagcacagtt gcctgcgcca cagcgatcga acttgccgtg
      781 acagtctcct gtcagaagga tgcgatggag gaagatgcat cgtcgcagca tgctgacgtc
      841 ccgccgcgac gatgcgaacc acggcatttg gggaagaatg acgcgtggtg ttacccggca
      901 atcacctcgc ccgatccccc aaacgcggcc ggaaagtcgc gcagcttggg caggccgtcg
      961 cgcatcggca gcacggtctc ggcgtagttg acgtgcacgt ccggcgtgaa cgtcagcgtc
     1021 ggcaacgtcg ccgtgaaaac atcgatcagg ccgagcggcg ggtgtttggt catcagatgg
     1081 ccgccacact tggtgcagaa cttgcgctgg cttagcggcg tcttctggaa cgtcgcgata
     1141 aattcggcgc ccgacgtgat gcgcactgcc tccggcttcc ataggctgaa ggcgttcacc
     1201 ggtccgcctg accaggaccg gcaagaattg cagtggcagt agcccatggc ttccggcgtg
     1261 ccggaaacct ccagtgccac cacaccgcag aagcattttc catagtgaac cacaacagac
     1321 ccttttctca ttcgctgaac tgcgttgaat ggtgaagcta cgcagcgcga aggatcagtg
     1381 catcgggaga gtttccctat tttgcggaaa tgatctcccc attttgcggc aatagatttt
     1441 gctcgcgcgc gatctctgct gcctcgatgc gagtcgttgc gccaagcttg gcaagcaccg
     1501 acgacacgtg gtgatcgacc gtcttcggcg acacatgcag tcgcgcaccg atccgcccgt
     1561 tgctgaggcc gatggccacg caggcgagga tttccatctc acgcaccgtc agaccgaacc
     1621 ggttgcgtcg cgtggtcgcg cgcggaccgc gcgggatgcg gctcatgcct tcgccacgca
     1681 tacgctgtcg caatacagcg gcagccggtg cggcgccgag acgatcgaag atctccagag
     1741 cggcgagttt tgcatcggtg tcgccatcgg ccagcgcacg tgcctgctca tacgggcaac
     1801 cgagtcgcgc ccactgttcg gcggctcgct gccagtcgcc ggacatctgc aaggtgaacg
     1861 gcgatgcgct ccaactgggt gcggaaacgg cgtcaccgca gaggtgccgc cagaaggcaa
     1921 attcgcccgc gtgccagcaa tgtgcggacc tgacggcaag gtcataggtg gcagtcgctt
     1981 cagcgattac ccgcggcgcg tcgcccgcga accaggctgc ttcggcgcgt gccgaacgga
     2041 cgggtgccag gcgttgcaac gtgtctgtct gcagcgcgag taccagcgct tcatcgagca
     2101 cctgctcggc acctggctcg ccgcgccgcg tgcgcagacg ccccaaggca ataagcgcca
     2161 ttatccgagt gatcgccgac gtgccaggcc gtccgacgac ccactcggcg agctccagag
     2221 cctcggtcca tcgcccttga tacaggcgca ccagcgcgag ccacgcagtc atgtagtgcg
     2281 tcgcgtgatc cagatcgcgt tcgcgcgcgt agtcgatgcc ttccgccagg tagcgctcgg
     2341 cttccgggaa caagtacacc tcgccatagc tggaaccgat gttcaggtag gcaagaccgg
     2401 caagcgcgtc ccaaccttcc tcgcgtgcca ttgcgagagc acggttcaaa tggtctgcac
     2461 cggcttcgtc gccatcca
//
LOCUS       DATGEM010000658         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1361933, whole genome shotgun sequence.
ACCESSION   DATGEM010000658 DATGEM010000000
VERSION     DATGEM010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361933"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VKQ27_20305"
     CDS             complement(<1..312)
                     /locus_tag="VKQ27_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HLY91338.1"
                     /translation="MLDELARTLQGRNAAVLVAPPGAGKTTRVPLALLEEPWTRAKKI
                     IVLEPRRIAARASAERMAKTLGERVGETVGYRVRFGSKISRATRIEVVTEGIFSRQIL
                     DD"
     gene            489..1601
                     /locus_tag="VKQ27_20310"
                     /pseudo
     CDS             489..1601
                     /locus_tag="VKQ27_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1830..2987)
                     /locus_tag="VKQ27_20315"
     CDS             complement(1830..2987)
                     /locus_tag="VKQ27_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HLY91339.1"
                     /translation="MKGLNFELGESIALLRETVRRFALHEIAPLAAQIDRENRYPRDI
                     WLKLGELGLLGITVDEEYGGSDLGYLAHVIAMEEISRASASIGLSYGAHSNLCVNQIR
                     RNGNEAQKRKYLPKLISGEHVGSLAMSEPGSGSDVVSMKTRAEKKGDRYVLNGSKMWI
                     TNGPVAETLVVYARTDPNAGPRGITAFIIEKGMKGFSTAQKLDKLGMRGSDTCELVFE
                     DCEVPQENVLGEVGHGVNVLMSGLDYERAVLAAGPLGIMQACMDVVLPYVHERKQFGQ
                     PIGTFQLVQGKVADMYTTMNASRAYVYAVAKACDRGETTREDAAGAILYAAEKATQCA
                     LDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKSA"
BASE COUNT          573 a          946 c          989 g          563 t
ORIGIN      
        1 atcgtcgagg atttgtcgcg aaaagatgcc ctcggtgacg acctcgatcc gcgtcgcgcg
       61 cgagattttg gagccgaagc ggacgcgata gccgaccgtc tcgccgacgc gctcgcccag
      121 cgtcttggcc atccgctcgg cgctggcgcg cgcggcgatc cggcgcggct ccagcacaat
      181 gatcttcttc gccctggtcc agggttcctc gagcagcgcc agcggtaccc gcgtggtctt
      241 gccggcaccg ggcggcgcca ccagcaccgc cgcgttgcgg ccctgcagcg tgcgcgcgag
      301 ctcgtcgagc accgcgtcga tcggaagggg cgtgtcgaag ctgcggggca aattgttgct
      361 cgcgttcggt tcttcccctc tccccttgtg ggagagggtg gcgaatggtc ctaaccggct
      421 acataggtaa cacaatagac cggcgacata ggtaacaggt caactggtcg gcgaggagga
      481 ccttgccgat gggatggatg gagacctgtg ccgtggaaga acggatgcgt tttatggcgg
      541 cggttgagac gggcgaggag tcgtttgccg cgatctgccg aaggttcgat gtgagccggc
      601 ggatcggata caagtggcga actcgctttg aggaggaggg ggctgccggc ctattcgacc
      661 gttcgcgagc tccgctgcat catccgcaag cgatcgcagg gcagatcgtc gagcgctgcc
      721 ttgaggtacg gcgggcgcat ccaacgtggg gaccggtgaa ggttctagcg tatctggagc
      781 ggcatgcgcc caagaccgat tggccggcgg cgagcacgat cggagagttg ttcgatcgcg
      841 agggactgac ggtgaagcgc cggcggcgcc ggcgctgccc gccatcgagt gtgccttttg
      901 cccattgtga agccgccaac gatgtctggt gcattgactt caaggggtgg ttcctaacgg
      961 gcgacggcgc gaggtgcgaa ccgctgacga ttacggacgc ccatagccgc tatcttctgc
     1021 gctgccaggc tctgcttcgc acggacactg accatgtctg gccggtgctg gaggctgcgt
     1081 tccgcgagtt cgggctgccc tgttacatgc ggtcggacaa cggatccccc tttgcctcgc
     1141 gcggtgccgg gggactatcg agattgtcgg tgaacctcat caaggctgga gttacgccgg
     1201 aacgcattgc ccccggcaag ccgcagcaaa acggccgtca tgaacgcatg catctcacgc
     1261 tgttgcagga agtcgccaac cctccggcac ccagcatgcg cgaacagctc aagcggctgc
     1321 gcaacttcca gcgttcgtac aacgaagagc ggccacacca agccctggac aatgcgacgc
     1381 cggccgaacg ctaccaggct tcagcgcggc gcttcgacgg catcctgcgc tcgccagaat
     1441 atggcgatca ggaggtccgc tgggttcgcc acaatggaga gatcaaactc gatggcaagt
     1501 tcatctatat caacgcggcc ttgatgggcg agcccattgg cctggctgaa tctgaggatg
     1561 gctggactgt gacctacggt cccatcacgc tcggcaccat cgcctaccga accgatcaat
     1621 tacgaagacc gaagcgcaag ggctgtggac ttggggacaa cgctgcgcgt agcccccagg
     1681 gtccacagcc ccaacaacaa caacaacaac aacaacaaca acaacaacaa caacagacct
     1741 gaacgaaacg ggaattgtgt tacccatgtc gccggtttga agtgttacct atgttggcgg
     1801 ttgcagaaga gcgagcgacg aatgcccttc taagccgatt tctcgaacag ctcgcgtccg
     1861 atcagcatgc ggcggatttc gctggtgccg gcgccgatct cgtagagctt ggcgtcgcgg
     1921 agcagccggc cggtcggata atcgttgata tagccattgc cgccgagcag ctggatcgcg
     1981 tccagcgcgc actgggttgc cttctccgcg gcatagagga tcgcgcccgc cgcgtcctcg
     2041 cgcgtggtct cgccgcggtc gcaggccttg gccaccgcgt agacataggc gcgcgaggca
     2101 ttcatggtcg tatacatatc ggcgaccttg ccctgcacca gctggaaggt gccgatcggc
     2161 tggccgaact gcttgcgctc atgcacataa ggcagcacca cgtccatgca ggcctgcatg
     2221 atgccgagcg ggcccgccgc cagcaccgcg cgctcatagt caaggccgct catcagcacg
     2281 ttgacgccgt ggccgacctc gccaagcacg ttctcttgcg gcacctcgca atcctcgaac
     2341 accagctcgc aggtgtcgga gccgcgcatg ccgagcttgt cgagcttttg cgcggtagag
     2401 aaccctttca tgcccttctc gatgatgaag gcggtgatgc cgcgcggccc cgcgttcgga
     2461 tcggtcctgg catagaccac cagcgtctcg gcgaccggac cgttggtgat ccacatcttg
     2521 ctgccgttca gcacataacg gtcgcccttc ttctcggcgc gggtcttcat cgacaccacg
     2581 tccgagccgg agcccggctc cgacatcgcc agcgagccga cgtgctcgcc ggaaatcagt
     2641 tttggcagat atttccgctt ctgcgcctca ttgccgttgc ggcggatctg gttgacgcag
     2701 aggttggaat gggcgccgta ggagaggccg atggaggcgg aagcgcggga aatctcttcc
     2761 atggcgatga cgtgggcgag gtagccaagg tcggagccgc cgtattcctc gtcgacggtg
     2821 atgcccagga gccccagttc gcctagtttg agccagatat cgcggggata gcggttctcg
     2881 cgatcgatct gggccgcgag gggcgcgatc tcgtgcaggg caaagcggcg cacggtctcc
     2941 cgcaggagcg cgatggactc gcccaattca aagttcaggc ctttcacatg cttctccttg
     3001 aggttcgatg catcagcgga gacctggcgc gatggggtta tccgggatgc ccggagccta
     3061 aaaggaaaga a
//
LOCUS       DATGEM010000659        17397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_636235, whole genome shotgun sequence.
ACCESSION   DATGEM010000659 DATGEM010000000
VERSION     DATGEM010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636235"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VKQ27_20320"
     CDS             <1..268
                     /locus_tag="VKQ27_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91340.1"
                     /translation="YSSEYNTGSDLDPGKSQKQYVLANARIAFGPHNDRWSIEMWAEN
                     LFDTDYKQVAFDSGFQNVPTNATGVLDAFLGAPRTFGGTLRLHY"
     gene            463..1482
                     /locus_tag="VKQ27_20325"
     CDS             463..1482
                     /locus_tag="VKQ27_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91341.1"
                     /translation="MIVRVEATPINPSDLGLLFGPADVTTMRSEGPADRPLLVADIPP
                     QRLRAVEGRIGQPMPVGNEGAGTVVRAGANVQSLLGRKVGMIGGGMYTQYRKLPAASC
                     MPLPDGASAADGAAMFVNPLTALGFVETMKAEGHSALVHTAAASNLGVTLNRICLADG
                     VPLVNIVRSEAQAASLRAIGARFVADSTSPDFRAELIDAVAATGATLGFDAVGGGKLA
                     GQILDAMEAAAARGSKGYSIYGSGSRKQVYIYGGLDTGPTIIDRTFGLSWELGGWLLT
                     PFLQKAGPETAARLRQRVADELLTTFASHYTAVISLSDVLRPEIAGAYQRKATGEKYL
                     IDPSR"
     gene            1688..2407
                     /locus_tag="VKQ27_20330"
     CDS             1688..2407
                     /locus_tag="VKQ27_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HLY91342.1"
                     /translation="MSDPALTPADVLAARRMPRVAPESAAYAQARQALLAEEIELRRH
                     IERVAAQRRALPPGPVIDKAYRFIDATGAEVGLADLFGDHQTLITYFWMYGPDRERPC
                     PMCTNLLGPLDANAADLAQKVAVAVLGRSPVERQLAFKAERGWRSLTFYQTSDDGFAT
                     DFGGLDRANGWEFPVLAVFGKEGNGTDARVRLFWMGEMSGEMADPGQDPRGAPDPAPL
                     WTMLDLTPDGRGADWYPKLTY"
     gene            2584..4524
                     /locus_tag="VKQ27_20335"
     CDS             2584..4524
                     /locus_tag="VKQ27_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HLY91343.1"
                     /translation="MTSDLFSDYIRNHEARRQAEMSMQDYLRDCRSDPLMYASAPERL
                     LAAIGQVQIIDTARDPRLGRIFMNRTIRTYPSFAEFYGMEETIEQIVAFFRHASQGLE
                     ERKQILYLLGPVGGGKSSLAERLKALMEVHPIYVLKAGDEISPVFESPLALFDPERNG
                     PQIEDQYGIPRRRLSGLVSPWCRKRLDDFGGDISRFKVVRLMPSRTHQIAIAKTEPGD
                     ENNQDISSLVGKVDIRKLELLSQNDPDAYSYSGGLNRANQGLLEFVEMFKAPIKMLHP
                     LLTATQEGNYIGTENIGAIPFSGIIMAHSNESEWQNFKNNKNNEAFIDRIFVIKVPYC
                     LRATEERQIYEKLLRDSDLSAAPCAPGTLDMLARFSVLTRLREHENSNLYSKMRVYDG
                     ETMREVDPRAKSQQEYKDAAGVDEGMAGTSTRFAFKALSATFNHDTTEVAADPVHLMY
                     VLERMIRQEQLQTEVETRYLEFVKGELAPRYAEFIGNEIQKAYLESYHDYGQNLFDRY
                     LAYADAWIEDLDFKDPDTGQLLDRDVINQELSKIEKPSGIANPKDFRNEVVKFALRMR
                     ASNQGRNPSWTSYEKIRDVIERRMFSQVEDLLPVISFGSKQDSETEKKHEEFVERMTT
                     RGYTERQVRRLVEWYMRVKQAG"
     gene            4546..5865
                     /locus_tag="VKQ27_20340"
     CDS             4546..5865
                     /locus_tag="VKQ27_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HLY91344.1"
                     /translation="MHVIDRRLNPGGKSLVNRQRFLRRAKALVQRAVRDALKDRSIRE
                     FDQEGEITIVRDGVHEPTLHQAGRGGDRDHVLPGNREYVEGDTIPRPEQGGGAGGSAA
                     GTGEGEDEFRFVLTREEFLDLFLEDLELPDLAKKRLTAGEVEGFRRAGYQTTGSPASL
                     SISRTMRNAMSRRLALKRPTSAELSRIEEEIERIERREPPDPGDAELLAKMREERDRI
                     ARRRLLISYIDPIDLRYRRYEETPKPVAQAVMFCLMDVSGSMTEHMKDLAKRFFALLH
                     LFLARSYRHVEVVFIRHTDRAAEVDENTFFYSRETGGTLVSSALDKMIEVVRARYRPD
                     DWNIYVAQASDGDNMAQDNPKAVNLLQEAILPIVQYAAYLEVGREEDPMAEGTMASES
                     DLWAAYQGVKAPKGRFVMRKVHHRREIYPVFRELFQRRGVGKQVPAR"
     gene            5862..7400
                     /locus_tag="VKQ27_20345"
     CDS             5862..7400
                     /locus_tag="VKQ27_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLY91345.1"
                     /translation="MNGKAGEPLFTGTDWDFGLINRIYEAVERIAVGEMKLDIYPNQI
                     EVITAEQMLDAYASTGMPLFYKHWSFGKQFLANELLYRKGVRGLAYELVINSDPCISY
                     IMEENSAALQTIVIAHAAFGHNHFFKNNYVFKQWTDAEGILDYLEFAKHYIRGCEERH
                     GDAAVEQLLDGAHALMHQGIHRYPRTRTRDLKAEAAREAERHAYQERIYDDLWRTLPR
                     GTKQAPAAAGERQQALQLPQENILYFLEKFAPKLAPWQREILRIIRLIAQYFYPQRQT
                     KLMNEGAATWCHYRILTTLHERGQLTDGAFMEFLQSHTNVVHQPTFDSGHYGGINPYA
                     LGFGIMRDIERICVSPTEEDRSWFPAIAGTGDAVSVLKDVWANYRDESFVAQFLSPHL
                     IREWRLFKLDDHEEAPSLHVDAIHDERGYRRIRHALMRHYDIGYQEPAIQIVDVDLAG
                     DRELRLEHSVNDGVLLAEADATKVLQHLADLWGYAVCLDEVDRQSGKQLKRHSAQPQV
                     VKPD"
     gene            7596..7853
                     /locus_tag="VKQ27_20350"
     CDS             7596..7853
                     /locus_tag="VKQ27_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91346.1"
                     /translation="MLLIPAALERRTGVCPMLTMESAAELLARAEMHEQLAAATDDIP
                     ARKMHQAMAAEYRRKAAEPGIISEIPPARIGPVLKLNVALE"
     gene            8176..8505
                     /locus_tag="VKQ27_20355"
     CDS             8176..8505
                     /locus_tag="VKQ27_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91347.1"
                     /translation="MTLLNLVLRHSWHDYATVERAMTFSLPMMIARLSMSGVSSFVGG
                     AVARWIDKTGWAPLVSGVILLVLFLPAHYSLWDRFPAWYHLTFLASLPLLGWLGGRLG
                     RPRPAMV"
     gene            complement(8518..8835)
                     /locus_tag="VKQ27_20360"
     CDS             complement(8518..8835)
                     /locus_tag="VKQ27_20360"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HLY91348.1"
                     /translation="MDSAHRFEVTARVWQWRPAEPSAAHWYFLTVEGQIAAELRYARM
                     QQTGTFGSIRVTVTIGSTRWQTSLFPERKSGNLLLPLKAEVRRRETIEAGDEVTAVIE
                     IQA"
     gene            complement(8904..8996)
                     /locus_tag="VKQ27_20365"
     tRNA            complement(8904..8996)
                     /locus_tag="VKQ27_20365"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8960..8962),aa:Ser,seq:gct)
     gene            9168..9923
                     /locus_tag="VKQ27_20370"
     CDS             9168..9923
                     /locus_tag="VKQ27_20370"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HLY91349.1"
                     /translation="MTIIVRRLGAAAGIAALAGLLAQPAAAQIRTVDPSSSQAHSDLA
                     SAGSGQPETSGAPAPRSDSYDTATPPAAAGRSDAHPPAVNASRAPDTQTYQENDVLDA
                     AEGVFGKGAEGLAKIIEKILKDQGRPTAYIAGREASGAFAIGLRYGSGTMHHKVEGDR
                     PAYWTGPSLGFDIGGNASKEFILVYNLYDSHDLYQRFPAAEGTAYFIGGFTASYLRSG
                     DIVLIPIRLGVGWRLGANVGYMKFTPESRILPF"
     gene            10026..10568
                     /locus_tag="VKQ27_20375"
     CDS             10026..10568
                     /locus_tag="VKQ27_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91350.1"
                     /translation="MAWHDRLRGYARRAGPLIALAGALALAGCYDDGYDGYGYGYAGG
                     AYGGYYGGDYGYAGFYDGWPGYGWYDNFYYPGSGYYAYDRHGGRHVWSGPRGQGWNGN
                     HGNWNGGRNWAGRGGHDGQGWAGRQGGNGGQGWGGRQGWNGNGGGRGMAAPSAGAQGR
                     QGGGGGRGRGGVQGGPPDRR"
     gene            complement(10578..11315)
                     /locus_tag="VKQ27_20380"
     CDS             complement(10578..11315)
                     /locus_tag="VKQ27_20380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLY91351.1"
                     /translation="MDLVNGTHVAIELAPDFAPVHVANIRTLVRAGWFDGSAVVRVQD
                     NYVVQWAGANEAKPLPASVVAHPPAEYDRPLAGLALRPLASPDAYAARVGHVEGWPVA
                     SDGGRAWLAHCYGMVGVGRDMPPDTGSGVELYGVIGQAPRHLDRNIALVGRIVSGIEA
                     LSALPRGTGDLGFYTDPAQRLGIRRTRIAADLPVAERPSYQVMRSDSPTFAAYVEARA
                     NRRDAFFVRPAQGVDLCNAPVPVRAQP"
     gene            11529..11619
                     /locus_tag="VKQ27_20385"
     tRNA            11529..11619
                     /locus_tag="VKQ27_20385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11563..11565,aa:Ser,seq:gga)
     gene            11771..12976
                     /locus_tag="VKQ27_20390"
     CDS             11771..12976
                     /locus_tag="VKQ27_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY91352.1"
                     /translation="MGKLSALKVRYLDKPGRYSDGDGLYLLIGPNGSKSWVLRVQVEG
                     KRRDIGLGSVKLRSLLEAREASIDMRRQLRGLATEPRQRPLKVRRKEVATVLTFKQAA
                     IQVHTERVKGWKNGKHQTQWLSSLEAYAFPTLGALPVDSVAAGQIHDVLAAIWLSKPE
                     TARRVSQRIGIVLDWAYAKGLRQAEAPMKSVGKGLPRQPKRSGHMMALRHADVPGFLA
                     ALREDRQGTGRLALEALILTAARSGEIRGARWDEVDLEQKEWVVPAERMKAGVTHVVP
                     LCDQAVDVFRRALKIRVPASDLVFPGQRPTQPLSDMTLLKILRDMEVAATVHGFRSSF
                     RDWAAEETDYPREIAEAALAHTVSNKVEGAYRRTDFLAKRRAMMADWGDYCEGKLRSA
                     SPSGVSSVV"
     gene            13220..13804
                     /locus_tag="VKQ27_20395"
     CDS             13220..13804
                     /locus_tag="VKQ27_20395"
                     /inference="COORDINATES: protein motif:HMM:NF040319.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HLY91353.1"
                     /translation="MKRVRGKGRGWVFTPKHFIDLGTRGSVDMALSRLAQAGSIRRIG
                     RGLYDYPRLHPKLGTLSPDANRIADALSIQTGDKLAPSGAAATNYLGLSTQVPAKASY
                     ATSGRTRTRKAAGRSVTLRHSRAPVLDQASDTGNSVLQALAHLGKQNIDDDIIRSFAR
                     RIDDRDMKVLAQGRPLMSGWMGDVILKIEAARNG"
     gene            13797..14870
                     /locus_tag="VKQ27_20400"
     CDS             13797..14870
                     /locus_tag="VKQ27_20400"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HLY91354.1"
                     /translation="MDDFARRDAADRRAYIEESANRRDLTPTIIEKDFWVCWTLRRLT
                     RFPELAGHITFKGGTSLSKAHGIIARFSEDIDLTIRRTAPLIEKIPSPMEEAISGNER
                     QRRTKALGTAAAEYVVTIAMPRLAAAIEDALGTGEGWSLELDPDDKAAQTLLFHYPRI
                     SGYGFSYGADYGGGAGGGYIKPRIKLEYGARGDPEPHEERSITPYLAEDFPGELPGAV
                     TAVETLSIARTFWEKATILHALHHSGKIRDGLSRHYYDLVMLERAGVGTGALEVPELL
                     GQVVRNKSLMFADAAASYGTAVPGTLRLVPADKDKVALSADYSAMADMFMVAPPPFDE
                     LMAELATLEAKLNADMAEAAVEA"
     gene            complement(14901..15101)
                     /locus_tag="VKQ27_20405"
     CDS             complement(14901..15101)
                     /locus_tag="VKQ27_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HLY91355.1"
                     /translation="MSDQPERILRIRTVLARTGLSRSTLYRKVGAGTFPRQVRIAERC
                     MGWRESAVNEWLRDTLVGQCQS"
     gene            15586..16506
                     /locus_tag="VKQ27_20410"
     CDS             15586..16506
                     /locus_tag="VKQ27_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HLY91356.1"
                     /translation="MSRKESGSSASSLYDQVTNRIIGELEAGRLPWVQPWDKAGVSAG
                     LPRNAGTGRSYSGINVLILWAAVMEHGYDRQAWITYRQAEALGGHVRKGEQGTTVCYA
                     DRFTPKAEQERAASAGDEARQIAFLKRFTVFNVDQCEGLPEALPGIVAPLPEREIIPH
                     AEALIDATGADFRIGGDRAFYAPGLDFVQVPFQQLYRVPIDWYRTAFHELGHWTGHAS
                     RLGRDLSGQFGDAFYAREELVAEMTSAFACAEMSIEPSVRHSDYIGSWLAVLREDNRA
                     IFRAASQASKAADFLLAFRAEAFSQMEDVA"
     gene            16503..16790
                     /locus_tag="VKQ27_20415"
     CDS             16503..16790
                     /locus_tag="VKQ27_20415"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HLY91357.1"
                     /translation="MSGDDPEGYVDPFTGERRAAPPPLPAPRYTVEDIDRALSQVPRF
                     EQRLFVLHRIDGLSYVEIAEQNGISVRRVEKAIARALTLTRRTLMKMGKWD"
     gene            16781..>17397
                     /locus_tag="VKQ27_20420"
     CDS             16781..>17397
                     /locus_tag="VKQ27_20420"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLY91358.1"
                     /translation="MGLIPDHSPRIRAGCALPLALLGMHVMRDQLDLLPASRQRELEH
                     VVRILFEEFEAATRTGNQPFTKQARILKLVLYGSFARGDWVADPVGGYHSDYDILVVV
                     NDERLTDFDYWSAAEDRLMREVTIAKTLTAPTNFIVHSLTDVNTQLYKGRPFFVDIVQ
                     QGVALYEAGGFPFSTPGKLPPEEALAEAQGHFERWFLSAEEFVFLA"
BASE COUNT         3165 a         5408 c         5695 g         3129 t
ORIGIN      
        1 atattcgtcc gagtataaca ccggttcgga tctcgatccc ggcaagagcc agaagcaata
       61 tgtcctggcc aacgcccgca tcgcttttgg cccgcacaac gatcgctgga gcatcgagat
      121 gtgggcggag aacctgttcg acaccgacta taagcaggtg gcgttcgact cgggcttcca
      181 gaacgtgccg accaacgcta ccggtgtgct ggatgcgttc ctcggtgcgc cgcggacgtt
      241 cggcggaacc ctgcgtttgc actactgagt tcccggcgta agctcggaat gggagacgag
      301 gccggatcgc ttccggcctc gtccctcgtt tgcagccaaa ggatcgggtg aaagatggac
      361 gaacaactgc aagggcttgc gcttcggtcg accgtcacgg ccgatgggct gctgcggctg
      421 agcctcgaaa ccgtccggtt cgatgagccc ggtccggatg aggtgatcgt ccgggtcgag
      481 gcgacaccga tcaacccgtc cgatctcggt ctgctgttcg gcccggccga cgtcacgacg
      541 atgcggagcg aagggccggc cgaccgcccg cttctggtcg ccgatatccc gccgcagcgc
      601 ctccgcgcgg tggaggggcg catcggccag cccatgccgg tgggcaacga aggcgccggc
      661 accgtcgtcc gggccggcgc caatgtgcag agcctgctcg gtcgcaaggt cggcatgatc
      721 ggcggcggca tgtatacgca gtaccgcaag cttcccgccg cgagctgcat gccgctcccg
      781 gacggcgcca gcgccgccga cggcgcggcg atgttcgtca atccgctgac ggcgctgggc
      841 ttcgtcgaaa ccatgaaggc cgaagggcac agcgcgctgg tgcacactgc tgcggcctcg
      901 aatctgggcg tgacgctcaa ccggatctgc ttggcggatg gcgttccgct ggtgaacatt
      961 gtgcgcagcg aggcgcaggc ggcgagtctg cgcgcgatcg gcgcccgttt cgtggccgac
     1021 agcacctcgc ccgatttccg cgccgagctg atcgacgcgg ttgccgcgac cggcgccacg
     1081 ctgggattcg acgcggtggg cggcggcaag ctcgctggcc agatcctcga cgcgatggag
     1141 gccgcggctg cccgcggcag caagggctac agcatctacg gatcgggatc gcgcaagcag
     1201 gtctatatct atggcgggct cgatacgggg ccgacgatca tcgatcgcac ctttggcctg
     1261 tcctgggagc tgggcggctg gctgctcacc ccgttcctcc agaaagccgg gccggaaacc
     1321 gctgcgcgcc tgcgccagcg tgtcgcggac gagctcctga ccaccttcgc aagccattat
     1381 accgcggtga tcagcctgtc ggacgtgctc cggcccgaga tcgccggcgc gtatcagcgc
     1441 aaggcgaccg gcgagaaata tctcatcgat ccctcgcggt agcggtcgat cgggcggcca
     1501 acccaaccct gtcagttccg gctcaaaacg gactcttgag cctcgcctca tgctccccgg
     1561 actcgatccg cgaagctgct cggggagccg gacgtgacgt gaggtcgccc gatgccgtcg
     1621 cggcgggcaa tccgcctgca acgggaactg tccgatcggt cttgggtttg gagcggtagg
     1681 aggcctcatg tccgatccag ccctgacgcc cgccgatgtg ctcgctgctc gccgcatgcc
     1741 gagagtcgcg cccgaaagcg ccgcctatgc ccaggcgcga caggccttgc ttgcagaaga
     1801 gatcgagctg cgccgccaca tcgagcgcgt cgccgcgcaa aggcgggcgc tgccgccggg
     1861 gccggtcatc gacaaggcct atcggttcat cgatgcgacg ggcgccgagg ttggccttgc
     1921 tgatctgttc ggtgaccatc agacgctgat cacctatttc tggatgtacg gacccgatcg
     1981 ggagcggccc tgcccgatgt gcaccaacct gttggggccg ctcgatgcca atgcggccga
     2041 tctggcgcag aaagtcgcgg tcgccgtgct cgggcggtct cccgtcgaac ggcagctcgc
     2101 gttcaaggcc gagcgtggct ggcgcagcct caccttctat caaacatccg acgatggctt
     2161 cgcgaccgac ttcggcgggc tcgaccgggc gaatggctgg gagtttccgg tgctcgcggt
     2221 gttcggcaag gaggggaatg gcaccgacgc ccgcgtccgg ttgttctgga tgggcgagat
     2281 gtcgggggag atggcagatc ccggtcagga tccgcgtggc gcgccggacc ccgcgccgct
     2341 gtggacgatg ctcgacctga cccccgacgg gcgcggcgcc gattggtatc ccaagctcac
     2401 ttattgaacg gggcgcggat gccggtccgc tcatcgcggc aagggtatgg gagttcatca
     2461 aaggcggcca gggcggacac tcccatatat gtttggttca tctctaggtt tccatgctgg
     2521 tttgtgccgg atagcgcacc tatatctcaa tgcgctccgc cgtctggaac gggagtcggc
     2581 attgtgacta gcgatctgtt ttcggactat attcggaatc acgaggcgcg gcgtcaggcc
     2641 gagatgtcga tgcaggacta tctgcgcgac tgtcgcagcg acccgctcat gtacgccagc
     2701 gcgcccgagc gattgctggc ggcgatcggg caggtccaga tcatcgacac cgcccgggat
     2761 ccacggctgg ggcggatttt catgaatcgc accatccgga cctatccgag cttcgccgaa
     2821 ttttacggga tggaggagac gatcgagcag atcgtcgcct tcttccgcca cgcatcgcag
     2881 ggattggagg agcgcaagca gatcctctac ctgttgggtc cggtgggcgg cggcaaatcc
     2941 tcgctggccg agcggttgaa ggcactgatg gaagttcatc ccatctatgt cctcaaagcc
     3001 ggcgacgaga tcagccccgt gttcgagagc ccgctcgcgc tgttcgatcc ggagaggaac
     3061 ggcccccaga tcgaggatca atacggaatt ccgcggcggc gcctctcggg gctggtgagc
     3121 ccgtggtgcc gcaagcggct cgatgacttc ggcggagaca tctcgcgctt caaggtggtg
     3181 cggctgatgc cgtcgcgcac gcaccagatc gccatcgcca agaccgagcc cggcgacgag
     3241 aacaaccagg acatctcttc gctcgtcggc aaggtggata tccgcaagct tgagctgctc
     3301 tcgcagaacg atcccgacgc ctacagctat tcgggcgggc tcaaccgcgc caatcagggc
     3361 ctcctcgagt tcgtcgagat gttcaaggcg ccgatcaaga tgctccaccc gctgctcacc
     3421 gcgacgcagg agggcaatta tatcggaacc gaaaatatcg gggcgatccc gttctccggc
     3481 atcatcatgg cccattccaa cgagtccgaa tggcagaatt tcaagaacaa caagaacaac
     3541 gaggcattca tcgaccgcat cttcgtgatc aaggtgccct attgcctgcg cgccaccgag
     3601 gaacggcaga tctatgagaa gctgctgcgc gattccgatc tttcagccgc gccatgcgca
     3661 ccgggcacgc tcgacatgct ggcgcgcttc tcggtgctga cgcggctgcg cgagcacgag
     3721 aattccaacc tctattcgaa gatgcgagtg tacgacggtg aaaccatgcg cgaggtggat
     3781 ccccgcgcca agagccagca ggaatataag gacgcggccg gcgtcgacga aggcatggcc
     3841 ggcacgtcga cacggttcgc cttcaaggcg ctgtcagcga cgttcaacca tgacaccact
     3901 gaggttgcgg ccgatcccgt ccacctgatg tatgtgctcg aacggatgat ccggcaggaa
     3961 cagcttcaga ccgaggtcga aacgcggtac ctggagttcg tgaagggcga gttggcgccg
     4021 cgttatgccg agttcatcgg caacgagatc cagaaggcct atctcgaatc ctatcatgat
     4081 tacggccaga atctgttcga tcgctatctc gcctatgccg atgcgtggat cgaggatctc
     4141 gacttcaagg atcccgatac cgggcagttg ctcgaccgcg atgtgatcaa ccaggagctc
     4201 tcgaagatcg agaagccctc gggcatcgcc aaccccaagg atttccgcaa cgaagtcgtc
     4261 aagttcgcgt tgcgaatgcg ggcgagcaat cagggccgca acccgtcctg gacctcctac
     4321 gagaagatcc gtgatgtgat cgagcggcgc atgttcagcc aggtggagga tctgctgccg
     4381 gtgatcagct tcggctcgaa gcaggatagc gagaccgaga agaagcacga ggagttcgtc
     4441 gagcggatga ccacgcgcgg ctataccgag cggcaggtcc gccggcttgt cgagtggtat
     4501 atgagggtca agcaggccgg ctaaaggagg cgggatcggg ggctgatgca cgttatcgac
     4561 cgacgtctga atcccggtgg gaagagcctg gtcaaccgcc agcgtttcct gcggcgggcc
     4621 aaggcgttgg tgcagcgcgc ggtacgggac gccctcaagg accgcagcat ccgagagttc
     4681 gatcaggagg gcgagatcac gatcgtgcgc gacggggtgc acgaaccgac gctccatcaa
     4741 gccggccgcg ggggcgatcg cgaccatgtt cttcccggca accgcgaata tgtcgagggc
     4801 gataccatcc cgcgcccgga gcagggcggc ggcgcaggcg ggtcggcagc cggcaccggt
     4861 gaaggcgagg acgagttccg cttcgtcctg acgcgcgagg aattcctcga tctcttcctc
     4921 gaggatctcg aactgcccga tctcgccaag aaacgcctaa ccgccggcga ggtcgagggt
     4981 ttccggcgcg cgggctacca gacgacggga tcgccggcga gcctctcgat ctcgcgcacg
     5041 atgcgcaatg cgatgtcgcg ccggctcgcg ctcaagcggc cgaccagcgc ggagctcagc
     5101 cggatcgagg aggagatcga gcggatcgaa cgccgcgagc cacccgatcc cggcgatgcg
     5161 gagctgctgg cgaaaatgcg cgaagaacgc gaccggattg cgcggcgacg cttgctgatc
     5221 tcctatatcg atccgatcga cctgcgttac cggcgctatg aagaaacgcc caaacccgtg
     5281 gcccaggcgg tgatgttctg cctgatggac gtctcgggct ccatgaccga acatatgaag
     5341 gacttggcca agcgtttctt cgcgctgctc cacctcttcc tcgcgcgcag ctaccgccac
     5401 gtcgaagtgg tcttcatccg ccacaccgat cgtgccgccg aggtcgacga gaacaccttc
     5461 ttctacagcc gggagacggg cggtacgctg gtgtcgagcg cgctcgacaa gatgatcgag
     5521 gtcgtccgtg ctcgctatcg acccgacgac tggaatatct atgtcgccca agcctccgac
     5581 ggcgacaata tggcgcagga caatcctaag gcggtgaacc tgctgcagga agcgatcctg
     5641 ccgatcgtgc aatatgcagc ctatctcgaa gtcgggcgcg aagaagatcc catggccgaa
     5701 ggcaccatgg ccagcgaaag cgacctgtgg gcggcctatc agggggtgaa ggcacccaag
     5761 gggcgcttcg tgatgcgcaa ggtgcatcat cgtcgcgaaa tctatccggt gttccgcgaa
     5821 ctgttccagc ggcgtggcgt gggcaaacag gtgccggcgc gatgaacggc aaggcaggcg
     5881 agccgctgtt taccggcacc gattgggatt tcgggctgat caaccgcatt tacgaggcgg
     5941 tcgagcggat agccgttggc gagatgaagc tcgacatcta tcccaaccag atcgaggtga
     6001 tcaccgccga gcagatgctg gacgcctacg cctcgacggg tatgccgctg ttctacaagc
     6061 actggtcgtt cggcaagcag ttcctcgcca acgagctgct ctatcgcaag ggcgtgcgtg
     6121 gtctggccta tgagcttgtg atcaattccg atccctgcat cagctacatc atggaagaaa
     6181 acagcgcagc gctgcaaacg atcgtcattg cgcacgcggc gtttggccac aatcatttct
     6241 tcaagaacaa ttatgtcttc aagcaatgga cggatgcaga gggcatcctc gattatctcg
     6301 aattcgccaa gcactatatc aggggctgcg aagagcgtca tggcgatgcc gcggtcgagc
     6361 agttgctgga tggcgcgcat gcgttgatgc accagggcat ccatcgttat ccgcgaaccc
     6421 gcacgcgtga cctgaaggcg gaagcggcgc gggaggctga gcgccacgcc tatcaggaac
     6481 ggatctatga cgacctgtgg cgtactttgc cacgcggtac gaaacaggcc ccggcggcag
     6541 ccggcgagcg ccagcaggca ctccagttgc cgcaagagaa tattctctat tttcttgaaa
     6601 aatttgctcc gaaactagcg ccttggcagc gcgagatcct gcgtatcatt cggctcatcg
     6661 cgcaatattt ctacccccag cggcaaacca aattgatgaa cgagggggcg gcaacctggt
     6721 gccactaccg catcctgacg acgctgcacg agcgcggcca gctcaccgac ggcgccttca
     6781 tggaatttct ccagtcgcat acgaatgtcg ttcatcagcc cacgttcgat tccggtcatt
     6841 atggcggtat caatccctat gcgctcggct tcgggatcat gcgcgacatc gaacgcatct
     6901 gcgtgagtcc gacggaggag gaccgcagtt ggttcccggc catcgcgggg actggcgatg
     6961 cggtttcggt gctcaaggat gtatgggcca attaccgcga tgagagcttc gtcgcccagt
     7021 tcctgagtcc gcatctcatc cgcgagtggc ggctgttcaa gctggacgac catgaggaag
     7081 cgccgtcgct ccacgtcgat gcaatccacg acgagcgtgg ctaccgcagg attcgccacg
     7141 cgttgatgcg gcattacgat atcggttatc aggaacccgc cattcagatc gtcgacgtcg
     7201 atcttgcggg cgatcgcgag ctcaggctcg agcacagcgt caacgatggg gtgctgctgg
     7261 ccgaggcgga cgccaccaag gttctgcagc acctcgctga tctttggggc tatgcggtct
     7321 gcctcgacga ggtcgatcgc caaagcggca agcaattgaa gcgccacagc gcgcaaccac
     7381 aggtggtgaa gccggattga tgagggcggg aggggcggca ggccgggaat ccggttccct
     7441 ttcgctggcg atcgcggtag agagacgggc tgatttggac cttccgggca gcgaatgcct
     7501 gtcggaagaa ggggagtttc gcctcaaacg aggtacgctg gtggaactag cagggacgca
     7561 aagtcgacgt agcggcatgc ggttcgagct taggggtgct gctgatcccc gcggctttgg
     7621 aacgacggac aggagtatgc cccatgctca cgatggaaag cgcagcggag cttctcgcgc
     7681 gcgcagagat gcacgaacaa ctcgccgcgg cgaccgatga tatcccggcg cggaaaatgc
     7741 atcaggcgat ggcggccgaa tatcgacgca aggctgcgga gcctgggatc atctcggaga
     7801 tccccccggc gcgcatcggc ccggtgttga aactcaacgt cgcgcttgaa tgacgctcgg
     7861 acacggccgt ccatgcggga gattttggcc tgcgaagcca ggctcatccc ggatgccgtc
     7921 attctggtca cctttcgggg atatgcctgt ccataggcgt tggccgggat cgcccggccg
     7981 acctcgagcc cgacagtcgc caagatgacc gtgatcgaaa cgttcggcac gcccggttca
     8041 tgctgaactg cgcgcatacc cggccgtctc acagatctgc tatcatgagc ggattgaata
     8101 ggatggggat ggggatgggg catgctgaga cggattggcg gagcggtcgt cggcgtcggc
     8161 gcctggattg tcgtcgtgac cctgttgaac ctggttcttc gccacagctg gcatgactat
     8221 gccaccgtcg aacgagccat gactttctcg cttcccatga tgatcgcgcg cctgtcgatg
     8281 agcggtgtca gttcattcgt cggcggtgcg gttgcccgtt ggatcgacaa aactggctgg
     8341 gccccgctcg tttcgggcgt gatcctcttg gtgctgttct tgcctgccca ttattcgctg
     8401 tgggacaggt ttccggcttg gtaccatctg acctttctgg cctctttgcc attgctgggc
     8461 tggttgggcg gcaggcttgg gcgccccagg cccgcaatgg tgtaacgagc cgccgaatta
     8521 agcctggatc tcgatcaccg cggttacttc gtcgcccgct tcgatagtct cccgtcgccg
     8581 gacttcagcc ttgagcggca gaaggaggtt tccgcttttc cgctcgggaa agagggaggt
     8641 ttgccaacgc gtgctgccga ttgtcacggt gacgcggatg gagccgaagg ttccggtctg
     8701 ttgcatccgc gcgtagcgaa gctctgcggc gatctgccct tccaccgtca gaaaatacca
     8761 atgcgcggcc gacggctccg caggacgcca ttgccagacc ctggctgtca cctcgaatcg
     8821 atgagcgctg tccattggcc ttgtcgctta gagtgatttc taagcggcct aagtgcctga
     8881 ttttaccgga aacctcaagc aaatggcgga gacggaggga ttcgaaccct cggtaggagt
     8941 aatcccccta cggcggttta gcaaaccgct ggtttcagcc actcacccac gtctccgggc
     9001 cggctccggc gggctatagc gacggttatc ggactgcaca acagcctgtt cgcgctgaat
     9061 cgtgcaaggc ttgagtcgtg tcgcgcgtgc cacccccggc gttcattgcg ctgcaaggtg
     9121 cttcgcggtt aacaggggag gatcagttgg gggagcagag gattaccatg accatcatcg
     9181 tccgtcggct tggggccgcc gccggtatcg ctgcgctggc agggctgctc gcccagcccg
     9241 ccgctgcgca gattcgcacg gtcgatccct cctcgtccca ggcgcattcc gatctggcgt
     9301 ccgccggcag cggccagccc gaaaccagcg gcgcccccgc tccgcgcagc gacagctatg
     9361 atacggccac gccgccggcg gctgcggggc gcagcgatgc gcacccgccc gcggtcaacg
     9421 cctcgcgcgc gcccgacacg cagacctatc aggaaaacga cgtcctcgac gccgccgagg
     9481 gtgtgttcgg caagggcgcc gaagggctcg ccaagatcat cgagaagatc ctgaaggatc
     9541 agggacgacc caccgcctat attgctgggc gtgaggcgag cggcgcgttc gcgatcggcc
     9601 tgcgctacgg ctcgggcacg atgcatcaca aggtagaagg cgatcggccg gcctattgga
     9661 cggggccgtc gctgggcttc gacatcggcg gcaacgcgtc gaaggaattc atcctcgtct
     9721 acaacctcta cgacagccat gatctctacc agcgcttccc ggcggcagag ggtaccgcct
     9781 atttcatcgg cggcttcacc gcgagctatc tgcgcagtgg cgacatcgtg ctgatcccga
     9841 tccgtctggg cgtcggctgg cggctgggcg ccaatgtcgg ctacatgaag ttcacgcctg
     9901 aatcgcgcat cctgccgttc tgatccacgc tctgccgcag gaacccaagg gggttcagca
     9961 ggggttcaag cccatgagac tagggtgttt ctcaagggcg gcccctctgt ggtggagacg
    10021 cgaggatggc atggcatgac aggttgcgtg gctatgcccg ccgcgccggg ccgctgatcg
    10081 cactggccgg cgcgctggcg ctcgccggct gctacgacga cggttatgac ggctacggct
    10141 acggctatgc cggcggtgcc tatggcggct attatggcgg tgattacggt tatgccggct
    10201 tctacgacgg ctggccgggc tatggctggt atgacaactt ctattatccg ggcagcggct
    10261 attacgccta tgatcgccac ggcggccggc atgtttggag cggtccgcgc ggccagggct
    10321 ggaacggaaa tcacggcaac tggaacggcg gtcgcaactg ggcgggccgc ggtggccacg
    10381 acggacaagg ctgggccggc cgacagggtg gaaatggcgg gcagggctgg ggtggccgtc
    10441 aaggctggaa cggcaatggc ggaggacgcg gcatggccgc gccatcggcc ggcgcgcagg
    10501 gtcggcaagg cggtggcggc ggccgtggtc gcgggggcgt tcagggtggg ccgcccgatc
    10561 gtcgctgagt cgccactcta gggctgtgcg cgcaccggga cgggagcgtt gcacaggtcg
    10621 acgccctgcg cggggcgcac gaagaaggcg tcgcgccggt tggcgcgcgc ctcgacatag
    10681 gctgcgaagg tgggggaatc gctgcgcatc acctggtagc ttgggcgctc ggcgactggc
    10741 agatcggccg cgatccgggt ccggcggatg cctagacgct gcgcgggatc ggtgtagaag
    10801 cccagatcac cggtgccgcg cggcagcgcg ctcagcgcct cgatcccgct gacgatccgc
    10861 ccgaccagcg cgatgttgcg gtcgaggtgg cgcggcgcct gcccaatcac gccatagagc
    10921 tcgacgcccg agccggtatc cggcggcatg tcgcggccca cccccaccat gccgtagcaa
    10981 tgcgcgagcc acgcgcgccc gccgtcgctc gccaccggcc agccttcgac atggccgacc
    11041 cgcgcggcat aggcgtcggg cgatgccagc ggtcgcagtg cgagcccggc cagcggtcga
    11101 tcatattcgg cgggcggatg cgcgaccacg ctggcgggga gcggcttggc ctcgttggcg
    11161 ccagcccatt gcaccacata attgtcctgc acgcgcacca ctgcgctgcc atcgaaccag
    11221 cctgcgcgca ccagcgtgcg gatattggcg acgtggacgg gcgcgaaatc gggcgcgagc
    11281 tcgatcgcga catgcgtgcc gttgacgaga tccatcagca gcagattgtc aggcgcgatc
    11341 ggccgccacg cgctggcggg tgctgcggcg atgatttctc ccggcgcgag tggcggggaa
    11401 gggggcgccg cggcgccgag cgtgagggcg aaaagggcgg agcacaggag agttcgacgc
    11461 ataagcgaag cctgccatag cgggggagct tgcggaagcg tcgcgatggc gctagtcgcc
    11521 ccccccacgg agcggtggcc gagtggtcga aggcgcacgc ctggaaagtg tgtaggggtc
    11581 caaagctcct cgagggttcg aatccctccc gctccgccaa cgacccatcc agacaaatcc
    11641 agaggcgttc ggacggccat tttttggctg aagtccgcca tttctgaatc catggccgtc
    11701 ccttgacgtt tgcgtaaatc cgtgactcaa tgtgggagaa aatgagggag cagaatcggg
    11761 agaaatccgg atgggcaagc tttctgcgct gaaagttcga tatctcgaca agcccggccg
    11821 ctacagcgac ggcgacggat tgtaccttct gatcggcccc aacggctcga aaagctgggt
    11881 gcttcgcgtc caggtcgagg gcaaacggcg cgacatcggc ctcggtagcg tgaagcttcg
    11941 ctcgcttctc gaggcacgtg aggcgagcat cgacatgcgc cgccagctgc gcggcctggc
    12001 gaccgaaccc cgccaacgac ccctcaaggt caggcgcaag gaggtggcca ccgttctgac
    12061 cttcaagcag gcggcgatcc aggtgcacac cgaacgcgtg aaaggctgga agaacggcaa
    12121 gcaccagacg cagtggcttt cctctctgga ggcttatgca tttccgactc tgggcgcgct
    12181 gcctgtcgat tcggtcgcgg ctggccagat ccatgatgtg ttggccgcga tctggttgtc
    12241 caagccggaa accgctcgcc gggtatctca acgaatcggg atcgtcctcg actgggctta
    12301 tgccaaaggc ttgcgccagg cagaggcgcc gatgaaatcg gtcggcaagg gcctgcctcg
    12361 ccaacccaag cggtccggcc atatgatggc actgaggcac gccgacgtac ccggcttcct
    12421 cgcggcactc cgtgaggacc gacagggcac cggcaggctc gcgctggagg cgctcatcct
    12481 gaccgcggcg cgatcaggcg aaatccgtgg cgcacggtgg gatgaagtcg acctggagca
    12541 gaaggagtgg gtggtcccgg ccgagcgcat gaaggcaggc gtcacccatg tcgtgccgct
    12601 gtgcgatcag gccgtcgatg ttttccggcg ggcgctgaag atccgggtac cggcgtccga
    12661 tctcgtcttt ccgggtcagc gaccaacgca accgttgtcg gacatgacgc tgctcaagat
    12721 cctgcgcgac atggaggtgg cggccaccgt tcacggcttc cggtccagct ttcgcgattg
    12781 ggcagccgaa gaaaccgact atcccaggga gatcgccgag gccgcgctcg cgcacaccgt
    12841 ctccaataag gtcgagggcg cctaccgtcg aacggacttc cttgccaagc gcagggccat
    12901 gatggcggat tggggcgatt actgtgaggg caagctgcga tcggcctcgc cgtcgggagt
    12961 ttcgagcgtg gtctgagtgg caatcttgct gaggcgactc gcggcgcatt gcccggatta
    13021 tctccgttct gccgcccgtg ccgcccgaag gcgatccgga ctcggatttg ttaggaaaat
    13081 tatgcgaaat ttctaacgag gctggaggcg catgtttctc tacgcatgtc gtcctcggcg
    13141 ggtggatagt tagaaaaaat accgctaata tctaacggaa aatgaggtgc tgcccgtgtc
    13201 tgccatggcc gatcgaatca tgaaaagagt gcgcggaaag gggcgcggat gggtgttcac
    13261 acccaagcat ttcatcgatc tcggcacgag aggctcggtc gacatggcac tctcgcgcct
    13321 cgctcaggcc ggcagtatcc ggcgtatcgg gcggggactg tacgactatc cccggcttca
    13381 tccgaagctc ggtacgctta gccccgatgc taaccgcatt gcggacgcgc tttcgattca
    13441 gacaggtgac aagcttgccc cgtcaggtgc cgcggcgaca aactatctgg gactttccac
    13501 gcaggtgccc gccaaggcga gctacgcgac cagtggccgc acgcggacca ggaaagccgc
    13561 cggtcgaagc gttacgctgc ggcacagccg cgcccccgtg ctcgaccagg catcagatac
    13621 gggcaactcc gtgcttcagg cgctcgcgca tctcggcaag cagaatatcg acgatgacat
    13681 catccgcagc ttcgcccgcc ggatcgatga tcgcgacatg aaggtgcttg cccagggacg
    13741 gccgctcatg tccggatgga tgggggatgt gatcctcaag attgaggctg ctcgcaatgg
    13801 atgatttcgc tcgccgggac gcggccgatc ggcgcgccta catcgaggaa tctgccaacc
    13861 ggcgcgacct taccccgacc attatcgaaa aggacttctg ggtctgctgg accctgcgcc
    13921 ggcttacccg ctttcccgaa cttgcgggcc atatcacctt caagggcggg acctcattgt
    13981 cgaaagccca cggcatcatc gcccggtttt cggaggacat tgatcttacg atccgccgga
    14041 ccgcgccatt gatcgagaag ataccctcgc cgatggagga ggccatcagc gggaacgagc
    14101 gtcagcgccg aacgaaagcg ctggggaccg ctgctgcgga atatgtcgtg accatcgcca
    14161 tgcccaggct ggctgccgcg atcgaagacg cgctcggcac cggcgagggt tggagccttg
    14221 agctggaccc cgatgataag gcagcgcaga cgctgctttt ccactatcct cgaatttcgg
    14281 gttatggctt cagctatggc gccgattacg gcggtggcgc aggcggcggc tacatcaagc
    14341 ccaggatcaa gcttgaatat ggcgcccgcg gcgatccgga gccccatgaa gagcgctcga
    14401 tcacacccta tctcgccgaa gattttcccg gtgaattgcc gggtgccgtc accgccgtcg
    14461 agacactcag catcgcgcgc accttctggg agaaggccac gatccttcat gcgctccatc
    14521 atagcggtaa gatccgggac ggtctttcgc ggcattatta cgacctggtg atgctcgagc
    14581 gcgctggtgt cggcaccggc gcgctcgagg tgcccgagct gctcgggcag gtcgttcgaa
    14641 acaaaagcct gatgttcgca gacgcggccg cctcctacgg caccgcggta ccgggcacgc
    14701 ttcgtctcgt cccggcagac aaggacaaag tagccttgtc cgcggactat tcggcgatgg
    14761 ctgacatgtt catggtcgcg ccaccgccat ttgatgagct gatggccgag ctggcaacgc
    14821 tcgaggcaaa gctcaacgct gatatggcag aggccgccgt ggaagcgtaa cacacgcacc
    14881 gacgatcgct tggcatccct tcaggactgg cactggccga ccagagtatc acggagccat
    14941 tcattgacgg cggattctcg ccagcccatg cagcgctcgg cgatccgcac ctgcctaggg
    15001 aatgtcccgg ccccgacctt tcggtagagc gtggaacggc tgagaccggt acgcgcgagc
    15061 accgttctga tgcgcaggat gcgctcgggc tgatcggaca tgcggaactc ccgtctgtgg
    15121 gttgaacgcg ggcgacgaga ttaagacgtc cgagttcgtc cctgaaccac cttgctgccc
    15181 attcttgccc attcttcccc tcgcgcgtcg gcggattcgg accgaggcgt cccgctcacg
    15241 cgaccgggct ctactccgct tcgcgctcca tcgagccgct ggcgcgtctc gccggcttcg
    15301 ccggcacggt ccctgacgca ggggccgccg ctttgcgccg gcatgcggcc atcgcgtgag
    15361 ccctcgcgat gagattctga tccggccgcg aggagggagc agggtgggcg tcgctgcccc
    15421 ttagtcccgc cgcggcgggc cgcaaaccgg ctgcgccggt tcctcctctc cgttccggtc
    15481 cttcggatgc tgcccgcctc ggtcggcggg actgccggtc cgctcctgcc gcccaccccg
    15541 cccccatccg gggccgggtg cggttgcgag gaaggagcaa ggatcatgag caggaaggag
    15601 tccggttcga gcgcatcgag cctctacgat caggtcacca accgcatcat tggcgagctg
    15661 gaagcggggc ggctgccatg ggtgcagcca tgggacaagg cgggtgtgtc ggcgggtctg
    15721 ccgcgcaatg ccggcaccgg gcgaagctat tcgggcatca atgtgctgat cctctgggcc
    15781 gccgtcatgg agcatggcta tgaccgtcag gcgtggatca cctaccggca ggccgaagcc
    15841 ttgggcggcc atgtccgcaa gggcgagcag ggtacgaccg tctgttatgc ggatcgcttc
    15901 acgccgaagg cggagcagga gagggcggcc agcgcgggcg acgaggcgag gcagatcgcc
    15961 ttcttgaagc gcttcaccgt gttcaatgtc gaccagtgcg agggcttgcc cgaggcgctg
    16021 cccggcattg tggcgccgct gcccgaacgt gagattatcc cgcatgccga ggcgctgatc
    16081 gatgcgaccg gagctgactt tcgcatcggt ggcgaccgcg ctttctatgc gccgggcctc
    16141 gactttgtgc aggtgccgtt tcaacagctc tatcgggttc cgattgattg gtaccgcacc
    16201 gccttccatg aactcggcca ctggacgggg catgcctcgc ggctcggccg tgatctctcc
    16261 ggccagtttg gcgatgcctt ctatgcgcgg gaagaactgg tggcagagat gaccagcgcc
    16321 tttgcctgtg ccgagatgtc gatcgagccc agcgtccgac acagcgacta tatcggctca
    16381 tggctggccg tgctgcgcga ggacaaccgc gcgatctttc gggcggccag ccaagccagc
    16441 aaggccgcag actttttgct cgccttccgt gccgaggcct tctcgcaaat ggaggacgtg
    16501 gcatgagcgg cgatgatccc gaggggtatg tcgatccctt cacaggtgag cggcgcgccg
    16561 cgccgccacc gcttcctgcg ccccgctaca cggtcgagga tatcgatcgg gcgctgtccc
    16621 aagtcccgag gttcgagcag cggctcttcg tcctgcaccg catcgatggc ctgtcctatg
    16681 tcgaaatcgc cgagcagaac ggcatctcgg tcaggcgggt ggagaaggcg atcgccaggg
    16741 cgctgacgct gacgcgccgc acactcatga agatgggcaa gtgggattga tcccggacca
    16801 cagcccgcgc atccgcgcgg gctgtgcatt gccgcttgcg ctgctaggaa tgcatgtgat
    16861 gcgtgatcag cttgatcttc ttccggcctc tcgccagcgc gagctggagc acgtcgtgcg
    16921 catcctcttc gaggaattcg aagccgcgac gcgcacgggc aaccagccct tcaccaagca
    16981 ggcgcgcatc ctgaaattgg tgctctacgg ctcgttcgca cgcggcgact gggtcgccga
    17041 tccggtcggc ggctatcatt ccgactatga catcctcgtc gtcgtcaacg acgagcggct
    17101 caccgatttc gattattggt cggccgccga ggaccggctg atgcgcgagg tgacgatcgc
    17161 caagacgctg accgcgccga ccaatttcat cgttcacagc ctcaccgatg tgaacaccca
    17221 gctctacaag ggacgaccgt tcttcgtcga tatcgtgcag cagggtgtcg ccctctatga
    17281 agccgggggt ttcccctttt cgacgcccgg aaagctgccg cccgaggagg cgctggccga
    17341 agcgcagggg cattttgaac ggtggttttt gagcgccgaa gagttcgttt ttttggc
//
LOCUS       DATGEM010000660         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_791286, whole genome shotgun sequence.
ACCESSION   DATGEM010000660 DATGEM010000000
VERSION     DATGEM010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791286"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1233
                     /gene="hflX"
                     /locus_tag="VKQ27_20425"
     CDS             10..1233
                     /gene="hflX"
                     /locus_tag="VKQ27_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLY91359.1"
                     /translation="MGPHNRTAEARLEEAAGLAGAIGLDVVERMAFRLRTPKAATLLG
                     SGQVEALAEAARMAEAELVVVDAAVTPIQQRNLEKAIEAKVIDRTGLILEIFGERAAT
                     AEGRLQVELAHLDYQAGRLVRSWTHLERQRGGFGFLGGPGETQIEADRRLIRDRMAKL
                     RRELEQVSRTRGLHRDRRRRAPWPVIALVGYTNAGKSTLFNRMTGADVMAKDLLFATL
                     DPTMRHIRLPGLDKAILSDTVGFVSDLPTQLVAAFRATLEEVTTADIIIHVRDMSHPD
                     TEAQAEDVAAVLAEIGATGEDGVPMIEAWNKVDLLDEESADALRAEAARRDDAVVLSA
                     LTGDGVNDLRRAVSDRLSRGARVRTVRLSASDGAAIAWLHQNGEVLATDTSGEIVSVD
                     VRISDVNWARFETRE"
     gene            complement(1273..1956)
                     /gene="gmk"
                     /locus_tag="VKQ27_20430"
     CDS             complement(1273..1956)
                     /gene="gmk"
                     /locus_tag="VKQ27_20430"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLY91360.1"
                     /translation="MAEPLDSAPIPSSRRRPHDTESQRRGVLFVISSPSGAGKSTIAR
                     KLIEVDGQIELSVSATTRPIRPGEVDGRDYHFVDTPEFKRMVADDEFLEWAHVFDHRY
                     GTPRAPVEEALAEGRDVLFDIDWQGAQQLYQQAAGDVVRVFILPPSMEELDRRLRGRG
                     TDSDAVIERRMERSVAEIGHWDGYDYVLVNDDVDRCFAQVRLILRAERLRRHRQRGLI
                     GFTRKLMRG"
     gene            complement(2081..2866)
                     /gene="mazG"
                     /locus_tag="VKQ27_20435"
     CDS             complement(2081..2866)
                     /gene="mazG"
                     /locus_tag="VKQ27_20435"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLY91361.1"
                     /translation="MVVEPVSPGAAIEQLAAVMAQLRDPQNGCPWDVAQDFATIAPYT
                     IEEAHEVADAIARGDMADLKGELGDLQLQVVFHARMAEEAGHFALADVIAGISDKMIR
                     RHPHIFGDTSRGDGVAVQSGWEAIKAQERADRQETGALAGVALGLPALMRAEKLQKRA
                     ARTGFDWPDPEGPRAKVTEELAELEGAPDDAARAEEFGDLLFALVNWGRHLGLDAEAA
                     LRSANAKFERRFAAMEAEAGEHFAGLSLDAKEALWQSAKARGL"
BASE COUNT          509 a          976 c          956 g          530 t
ORIGIN      
        1 ctccctgaga taggcccgca taatcgtact gccgaagcgc gactggagga ggcggccggc
       61 ctcgccggtg cgatcggcct cgatgtcgtc gagcgcatgg cctttcgctt gcgcacaccg
      121 aaggctgcga cattgctcgg ctcgggccaa gtcgaggctc tcgccgaggc cgcgcgcatg
      181 gccgaggcag agcttgtcgt cgtcgatgcg gccgtcacgc ccatccagca gcgaaacctg
      241 gagaaggcga tcgaggcgaa ggtaatcgat cgcaccgggc tgatcctcga aatcttcgga
      301 gagcgcgcgg cgactgcgga aggccggctc caggtcgagc tcgcgcatct cgattatcag
      361 gctggtcggc tggtccgaag ctggacccac cttgaacgcc agcgcggtgg tttcggcttc
      421 ctgggtggcc ccggtgaaac gcagatcgag gcggaccgtc ggctgatccg cgatcgcatg
      481 gccaagctcc gtcgtgaact cgagcaggtg agccgaacgc gcggcctcca tcgtgatcga
      541 cgacggcgcg cgccctggcc ggtgatcgcg ctcgtcggct atacgaacgc ggggaaatcc
      601 acacttttca atcggatgac gggagctgac gtcatggcga aggatttgct cttcgctacg
      661 ctcgatccca ccatgcgcca tatccggttg cccgggctcg acaaggcgat cctgtccgac
      721 acagtgggtt tcgtgtccga tcttccaacg cagcttgtcg cggcgttccg cgccacgctc
      781 gaagaggtga cgacggccga tatcatcatc catgtccgag acatgtcgca tccggacacc
      841 gaagcgcagg cggaggatgt tgccgcggtc ctcgccgaga tcggtgcgac cggcgaagac
      901 ggtgtgccga tgatcgaggc ctggaacaag gtcgatctgc tcgacgaaga atctgctgac
      961 gcgctccgtg ccgaagccgc ccgacgggac gatgcggtgg tgttgtcagc gctgaccggg
     1021 gacggcgtca atgaccttcg ccgcgctgtc tcggaccggc tcagccgtgg cgcgcgcgtc
     1081 cgcaccgttc gcctctcggc gagcgatgga gccgcgatcg cgtggctgca tcagaatggc
     1141 gaagtgctcg caaccgacac cagcggcgag atcgtaagcg tcgatgtgcg gatcagcgac
     1201 gtgaactggg cacgcttcga aacacgcgaa taggccagcg ccctctccgt ttgcgcgggg
     1261 agggcgccga ttttatccgc gcatcagttt ccgggtgaag ccgatcaggc cacgctggcg
     1321 atggcggcgc agtcgctccg cgcgcaggat caggcgcacc tgggcgaaac agcgatcgac
     1381 gtcgtcattg acgagcacat agtcgtagcc gtcccagtgc ccgatctcgg cgacggaacg
     1441 ctccatgcgg cgttcgatca ccgcatcgct gtcggtgccg cggccgcgca ggcgacgatc
     1501 cagttcttcc atcgacggcg gcaggatgaa cacgcgcacc acatcgccgg cggcctgctg
     1561 gtagagctgc tgcgcgccct gccaatcgat atcgaacaac acgtcgcggc cttccgccag
     1621 cgcctcctcg acgggtgcgc gcggggtgcc gtagcggtga tcgaagacgt gcgcccattc
     1681 caggaactca tcatcggcga ccatccgctt gaattcggga gtgtcgacga agtgatagtc
     1741 gcggccgtcc acttcgccgg gccgaatcgg ccgcgtggta gcggagacgg atagctcgat
     1801 ctgtccgtcg acttcgatca atttgcgcgc gatcgtcgac ttccccgcgc cagatggcga
     1861 cgagatgacg aacagcactc cgcgacgctg cgattccgta tcgtgcggac ggcggcggga
     1921 agacgggata ggggcggaat caaggggttc ggccatggcc gcttctgccg tgtcgcagca
     1981 tagcccgtca atcctcgacg ttgtgagccc attcggatcg atttgcgcca ttcccgtggg
     2041 cttgtcttga ggcgggtatg cgtgcgacgc tggcattgac ctagaggcca cgtgccttcg
     2101 cgctctgcca aagcgcctcc ttcgcatcaa ggctcagccc ggcaaagtgc tctccggcct
     2161 cagcctccat cgccgcaaaa cgacgctcga acttcgcgtt ggcggaccgc agcgccgcct
     2221 ccgcatcgag gccgagatgg cggccccaat tgaccagcgc aaacaaaagg tcgccgaact
     2281 cctctgcgcg ggccgcatca tcgggcgcgc cttccagttc ggcgagctct tcggtcacct
     2341 tggcgcgtgg cccctcggga tcaggccagt cgaatcccgt gcgtgccgca cgcttctgca
     2401 gtttttcagc ccgcatcagc gcgggcaggc caagcgccac gcccgccagc gcgccggttt
     2461 cctgtcgatc cgcgcgctct tgcgccttga tcgcttccca gccgctttgc acggccacgc
     2521 cgtcgccgcg cgacgtgtcc ccgaagatgt gcggatggcg gcggatcatc ttgtcggaga
     2581 tgcccgcaat cacgtcggcc agcgcgaaat ggccggcttc ctctgccatg cgggcgtgaa
     2641 acaccacctg gagctgaaga tcgccgagtt cacccttgag atcggccata tcgccacgcg
     2701 cgatagcgtc cgccacttcg tgcgcttctt cgatcgtgta gggcgcgatc gtagcaaagt
     2761 cctgagcgac gtcccacgga cacccgttct ggggatcgcg tagctgcgcc atcacggctg
     2821 cgagctgttc gatcgcggcc cccggagaga cgggctcgac caccattgtc atgctcgccg
     2881 ggcctgaaac agacgctcga tcgatgctga tgcctgttcg cgcaaagttg gaagtctcat
     2941 ctcgtgcaaa gtacagatgt cccttcgtct t
//
LOCUS       DATGEM010000661         6045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_865996, whole genome shotgun sequence.
ACCESSION   DATGEM010000661 DATGEM010000000
VERSION     DATGEM010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865996"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VKQ27_20440"
     CDS             complement(<1..912)
                     /locus_tag="VKQ27_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HLY91362.1"
                     /translation="MFRTLLIANRGEIACRIVRTARSMGIATVAVYSDADANALHVRT
                     ADSAVLIGPPPAGQSYLNIAAIIDAARRSGADAIHPGYGFLSENPAFAEACADAGIVF
                     VGPPPDAMRAMGSKSAAKTLMEQAGVPLLPGYHGENQAPDFLAHQAARVGFPVMIKAV
                     SGGGGRGMRIVTAANDFAAALTAAQQEAASAFGDDRVLIERYLDRPRHIEVQVFADSL
                     GNVVHLFERDCSVQRRHQKVIEEAPAPGLDAKRRAAIGGAAVAAAKAVGYQGAGTVEF
                     VANADGFFFLEMNTRLQVEHPVTEMITG"
     gene            complement(918..2507)
                     /locus_tag="VKQ27_20445"
     CDS             complement(918..2507)
                     /locus_tag="VKQ27_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLY91363.1"
                     /translation="MTVLHSDLNPRSDEFRANAEAMRALVDDLRDRVAEVRQGGGEQA
                     RQRHIARNKLLARDRVAGLIDPGSPFLELSQLAAYGLYDNEVPSAGIVTGIGCVSGRE
                     CVIVANDATVKGGTYFPLTVKKHLRAQEIARQNRLPCLYLVDSGGAFLPMQDEIFPDR
                     DHFGRIFFNQANLSAQGVPQIAAVMGSCTAGGAYVPAMSDETIIVRKQGTIFLGGPPL
                     VQAATGEIVSAEDLGGADVHARTSGVVDHYARDDHHALALCRRIVAGLNHAKRPPVVT
                     RQPQLPRHDPTELYGVVPLDLRAPYDARELIARVVDASEFDEFKRLYGTTLICGFAHI
                     HGYPVGILANNGILFSESALKGAHFIELCSQRGIPLLFLQNITGFMVGRKYEAGGIAK
                     DGAKLVTAVSTTSVPKITVIVGGSYGAGNYGMCGRAYEPRFLFMWPNARISVMGGEQA
                     AMVLSQVRRGAERSPEEDEAFKAPIRAQYERQGHPYYATARLWDDGIIDPADTRRVVG
                     LALSAALNAPIEATKFGVFRM"
     gene            complement(2504..3682)
                     /locus_tag="VKQ27_20450"
     CDS             complement(2504..3682)
                     /locus_tag="VKQ27_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HLY91364.1"
                     /translation="MASLPNRLPGLNFDLGEAADMLRGTVEAFAGDEITPRAASIDRD
                     NAFPMDLWRKFGDLGVLGITVEEEYGGAGMGYLEHVVAMEEISRASASVGLSYGAHSN
                     LCVNQLRRNGSEAQKRRYLPRLINGEHVGALAMSEPGAGSDVVGMRTRADKRGDRFVL
                     NGSKMWITNGPDADVLVIYAKTTPDAGPRGITAFIVEKSFKGFSTAQKLDKLGMRGSN
                     TCELVFTDCEVPEENVLGSVDDGVRVLMSGLDYERAVLAAGPLGIMQACMDAVMPYVH
                     ERKQFGQPIGEFQLMQGKIADMYTTMNAAKAYVYTVAKACDRGEATRKDAAGAILYAA
                     EKATWMALEAIQCLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFRETA
                     "
     gene            complement(3686..4009)
                     /locus_tag="VKQ27_20455"
     CDS             complement(3686..4009)
                     /locus_tag="VKQ27_20455"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLY91365.1"
                     /translation="MFIAMNRFKVARGSEADFEKVWTSRDSHLGGVPGFGTFHLLKGP
                     EYDDHVLYSSHTVWRSKADFEAWTRSEAFRRAHSDAGSNRTLYLGHPEFEGFEVIQTI
                     EGGKG"
     gene            4096..4980
                     /locus_tag="VKQ27_20460"
     CDS             4096..4980
                     /locus_tag="VKQ27_20460"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY91366.1"
                     /translation="MAVSFNQLHQAFAAEVAGIDCRKPLTHDEVAAVEAGMDKYAVLV
                     FRDQQITDDEQLAFTLHFGSLENYATGGHIRKDVDSRLRSGMADFSNLDKHGNIMSAQ
                     DRIWFFKLGDRLWHSDSSFRPVPARYSLLSGRVLPSWGANTEFADMRAAYDALDARTK
                     AEVEDLVCLHSLMFSRAAIGFTELSEQEIAAFRPVRQRLVRTHPVTGRKSLFLSSHAG
                     AIEGWTIPEARMFLRDLTEFATQREFVYSHQWQPFDLVIWDNRTTMHRARRFDRNEVR
                     DVRRTTLAGDVITIEQHA"
     gene            5238..>6045
                     /locus_tag="VKQ27_20465"
     CDS             5238..>6045
                     /locus_tag="VKQ27_20465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY91367.1"
                     /translation="MSDSAGHVVIVGAGHAGGSAAAFLRQYGWKGPITLMGAEPVLPY
                     QRPPLSKTWLTGDGDLASLLLRSADFYRTNSIVWRADAEVMAIDRSGRAIALASGERV
                     DYDQLILALGSVVRALPLPGHDLRNIVTLRTVADADRLKTLLSPGVRLAIVGAGYIGL
                     EIAASARSLGADVIVLEREGRVLPRVASEPLSAFFQERHSAAGVRIMLNASLEAFEGD
                     GAIAGIRLRDGTVIACNAALVGIGAAPNDALARSAGLTCLNGIVVNQAART"
BASE COUNT         1124 a         2041 c         1802 g         1078 t
ORIGIN      
        1 cccggtgatc atctcggtca ccggatgctc aacctgaagg cgcgtattca tctccaggaa
       61 gaagaagccg tctgcattgg cgacgaactc cacagtgcct gcgccctggt agccaacggc
      121 cttggcagcg gcgaccgcgg ccccccctat ggcggcgcgc cgcttcgcat ccagccctgg
      181 cgcgggcgct tcctcgatca ccttctggtg gcgtcgctgt acggagcagt cacgctcgaa
      241 cagatgcacc acgttgccga gcgagtctgc gaagacctgc acctcgatat ggcgaggccg
      301 atccaggtac cgctcgatca gaacgcggtc atcgccaaaa gcagacgcag cttcctgctg
      361 tgcggcagtc agcgcggcgg caaagtcgtt cgcggcggtc acgatgcgca taccgcgccc
      421 gccaccaccg gaaacggcct tgatcatgac cggaaagcca acccgcgccg cctggtgcgc
      481 caggaaatca ggagcctgat tttcgccgtg atacccgggc agcaatggca cgccagcctg
      541 ctccatcagc gtcttcgcgg ctgacttcga tcccatcgct cgcatcgcat ccggcggcgg
      601 cccgacaaag acgatgccgg catcagcgca cgcctccgcg aacgcgggat tctccgacag
      661 gaagccgtag ccgggatgga tcgcatcagc accgctccgc cgcgcggcat cgatgatggc
      721 cgcaatgttc agatagctct gccccgcggg tggcggcccg atcagcaccg cgctgtctgc
      781 cgtgcgcaca tgcagcgcat tcgcatccgc gtccgaatag acggcaacgg tcgcgatgcc
      841 catgctccgc gcagtgcgga caatccggca ggcaatttcg ccgcggttgg cgatgagcag
      901 tgtccggaac atcaccgcta catccggaac acgccaaact tcgtcgcttc gatcggcgca
      961 ttcagcgcgg cagaaagcgc gagcccgaca acgcggcgcg tatccgccgg atcaataatg
     1021 ccgtcgtccc agaggcgcgc agtcgcgtag tacggatgcc cctgccgttc gtactgcgcg
     1081 cgaatcgggg ccttgaaggc ttcgtcttcc tccgggcttc gctcggcgcc gcgacggacc
     1141 tgcgacagca ccatcgccgc ctgttcgcca cccatgacgg aaatacgcgc attcggccac
     1201 atgaacagga aacgaggctc gtatgcacgg ccgcacatac cgtagttccc agcgccatag
     1261 ctgccgccaa cgatcaccgt gatcttcggc accgacgtcg tggagaccgc ggtgaccagc
     1321 ttcgcgccat ccttggcaat gccgccagcc tcgtatttgc ggcccaccat gaagccggtg
     1381 atgttctgca ggaacagcag aggaatgccg cgctggctgc acaattcgat gaagtgcgcg
     1441 cctttcagcg ccgattccga aaacaggatg ccgttgtttg ccagaatgcc aaccgggtag
     1501 ccgtggatgt gcgcgaaccc acagatcagc gtggtgccat acagccgctt gaactcatcg
     1561 aactcgctcg catcgacaac gcgcgcgatc agctcccgcg catcgtacgg cgcgcgcaga
     1621 tccagcggaa ccacaccata cagctcggtc ggatcatggc gcggcaactg cggttgcctg
     1681 gtgacgacag gcggacgctt ggcgtggttt aacccggcaa cgatacgccg acacagcgcg
     1741 agcgcgtggt gatcgtcgcg tgcatagtga tccaccacgc ccgacgtgcg tgcatgcaca
     1801 tcggcaccgc ccagatcctc cgcgctgaca atctcgccgg tcgccgcctg caccagcgga
     1861 ggaccaccaa ggaatatcgt gccctgcttg cgcacgatga tcgtctcgtc actcatcgcc
     1921 ggcacatagg caccgcccgc ggtgcaggat cccatcaccg cggcgatctg cgggacacct
     1981 tgtgccgaca gattggcctg gttgaagaag atgcgaccga agtggtcgcg atcgggaaag
     2041 atctcatcct gcatcggcag aaaggcgccg ccagaatcaa ccaggtatag acaaggcagc
     2101 cggttctgcc gcgcaatctc ctgcgcccgc aggtgcttct tcaccgtcag cggaaaatat
     2161 gtgccgccct tcaccgtcgc gtcattagcg acgatgacac actcgcgacc ggagacgcag
     2221 ccaatgccgg tcacgatgcc ggcggacggc acttcattgt catacagccc ataggcggca
     2281 agctgagaca gttccaggaa cggcgagcca ggatcgatca gcccagccac acgatcccgc
     2341 gccaacagtt tgttgcgggc aatatgccgc tgacgcgcct gttccccgcc cccctgccgc
     2401 acctcggcaa cacgatcccg cagatcgtcc accagcgcac gcatcgcctc ggcgttggcg
     2461 cggaattcat cggatcgcgg attgagatcg gaatgcagga ccgtcatgcg gtttcgcgga
     2521 acagctcgcg gccgatcagc atgcggcgta tctcgctggt gcccgcgcca atctcataca
     2581 gcttggcgtc ccgcagcagg cggccggtgg gatagtcgtt gatatatccg ttgccgccga
     2641 ggcactggat cgcctccagc gccatccacg tggccttctc cgccgcatag aggatagcgc
     2701 ccgcagcgtc cttgcgcgtg gcctcgccgc gatcgcaggc tttcgccacc gtatacacat
     2761 acgccttggc ggcattcatc gtcgtgtaca tatcggcgat cttgccctgc ataagctgga
     2821 actcgccgat cggctggccg aactgcttgc gctcatgcac gtacggcatc accgcgtcca
     2881 tgcaggcctg catgatgccc agcggcccgg ccgccagcac ggcgcgctcg taatccagcc
     2941 cgctcatcag cacgcgaacg ccgtcgtcga cactgcccag cacgttctct tccggcacct
     3001 cgcagtcggt gaacaccagc tcacaggtgt tcgaaccgcg catgcccagc ttgtcgagct
     3061 tttgcgcggt agagaaaccc ttgaagctct tttcgacgat gaacgcggtg atgccgcgcg
     3121 gcccggcatc cggcgtggtc ttggcgtaga tcaccagtac atcggcatcc ggcccgttgg
     3181 tgatccacat cttcgatccg ttcagcacga accggtcgcc gcgcttgtcg gcacgtgtgc
     3241 gcatgcccac cacgtcggac ccggcgcccg gttcgctcat cgccagcgcg ccgacatgct
     3301 cgccattgat caatcgcggc aggtagcgtc gcttctgtgc ctcgctgcca ttgcggcgga
     3361 gctggttgac gcagagattg gagtgcgcgc catacgacag gccaacggaa gcagatgcgc
     3421 gcgatatctc ctccatcgcc acaacatgtt ccagatagcc catgccggcg ccgccgtatt
     3481 cctcctccac cgtgatcccc agcacgccga ggtcaccgaa cttgcgccac agatccatcg
     3541 ggaacgcgtt gtcgcgatcg atgctcgcgg cgcgcggcgt gatttcgtcg ccggcgaacg
     3601 cctcgaccgt gccccgcagc atgtccgccg cttcgccaag gtcgaagttc aggcccggaa
     3661 gccggtttgg cagcgacgcc atcgctcagc ctttgccgcc ttcgatcgtc tggatcacct
     3721 cgaagccctc gaattccggg tgacccagat acagggtccg gttgctgccg gcatcgctat
     3781 gcgcgcggcg gaatgcctcg gagcgcgtcc acgcctcgaa gtccgccttg ctgcgccaca
     3841 ccgtgtgcga cgaatacagc acgtggtcgt cgtactcggg ccccttcagc agatggaatg
     3901 tgccaaagcc cggcacgcca cccagatggc tgtcgcgcga cgtccacacc ttctcgaagt
     3961 ctgcttccga gcctcgcgcg accttgaacc ggttcattgc aatgaacatg gcgccaccat
     4021 tgcttgcctc ggcttcccat accctggacg gcgcccacgg gaaagatttc aataccgggg
     4081 gcttgggagg gcgacatggc ggtttcgttc aatcaactgc atcaggcgtt cgccgcggaa
     4141 gtcgcgggga tcgattgccg caagccgttg acgcatgacg aggtcgccgc ggtcgaagcc
     4201 gggatggaca aatacgccgt cctggtgttc cgcgaccagc agatcaccga cgacgagcag
     4261 ctcgccttca ccctgcattt cggctcgctg gagaactatg cgaccggcgg ccatatccga
     4321 aaggacgtgg acagccggct gcgatccggg atggccgact tctccaacct ggacaagcac
     4381 ggcaacatca tgtctgccca ggaccgcatc tggttcttca agctcggcga tcggctgtgg
     4441 cattccgaca gctccttccg tcccgtcccc gcgcgctatt cgctgctgtc gggtcgggtc
     4501 ctgccgtcgt ggggcgccaa caccgaattc gctgacatgc gcgccgcata cgatgcgctc
     4561 gatgcgcgca ccaaggccga ggtggaagat ctggtctgcc tgcactcgct gatgttctca
     4621 cgcgcggcca tcggtttcac cgagctctcc gagcaggaaa tcgccgcctt caggccggta
     4681 cgccagcgcc ttgtgcgcac gcatccggtg acaggccgca aatcgttgtt cctgtcgtca
     4741 cacgccggtg cgatcgaagg ctggaccata cccgaagcgc gcatgttcct gcgtgacctg
     4801 accgaattcg cgacacagcg cgagttcgtc tactcgcacc aatggcagcc attcgacctg
     4861 gtgatttggg acaaccgcac caccatgcac cgcgcacgcc gtttcgatcg gaacgaagtg
     4921 cgcgatgtcc gccgcacaac cctggccggc gatgtgatca cgatcgagca gcatgcatag
     4981 ttcttgactt taacctgtca tacagcgcaa actttcggcc cacgtcgaaa ggagggtgca
     5041 tagacagcat gactccaccg ggcaaacaaa gtgttgccga ccgacgtggc atcggcaact
     5101 gaatcataag tccacggatg tacccggcga tcagggccgg gccaaagaag cagatgcttc
     5161 gcaatgtgac ccccgatcgg cgctcaggcg cgcgggggtc tttgcgcatc aaccattcca
     5221 tccgctaacg tgattgcgtg agcgatagcg caggacacgt ggtgatagtt ggtgccggcc
     5281 acgccggcgg gtccgcagcg gccttcctcc gtcaatatgg ctggaaggga ccgatcacat
     5341 tgatgggcgc ggagccggtg ctcccctacc agcggccacc gctatcgaag acctggctga
     5401 ccggcgacgg cgatctcgca agcctgttgc tgcgctctgc cgatttctat cgtaccaatt
     5461 cgatcgtgtg gcgcgccgat gccgaagtga tggccatcga ccgctccggc cgcgccatcg
     5521 ctctcgcaag tggcgaacgc gtggattacg atcagttgat cctggcgctg ggatcagtcg
     5581 tgcgcgcctt gccgctgccc ggacatgatc tgcgcaatat tgtgacattg cgcacggttg
     5641 ccgatgcaga tcggctaaag acgctgctgt cgccgggcgt gcggctcgcg atcgtaggcg
     5701 ccggctatat cgggctggag atcgcagcct cggcgcgctc gctgggggcg gacgtcattg
     5761 tgcttgaacg cgaaggccgt gtgctgccac gcgtcgcatc agagccattg tcggcattct
     5821 tccaggagcg ccactcagca gcaggcgtga ggatcatgct gaatgcctcc ctcgaagcat
     5881 tcgagggtga cggagccatc gccggcatcc gccttcgcga tggcacggtc atcgcgtgca
     5941 atgccgcgtt ggtcggtatc ggtgcagcac cgaacgacgc cctggcgcgc tcggccggat
     6001 taacatgcct caacgggata gtcgtgaatc aggcagcacg tacca
//
LOCUS       DATGEM010000662         5285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1301894, whole genome shotgun sequence.
ACCESSION   DATGEM010000662 DATGEM010000000
VERSION     DATGEM010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301894"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VKQ27_20470"
     CDS             <1..1373
                     /locus_tag="VKQ27_20470"
                     /inference="COORDINATES: protein motif:HMM:NF004808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005324 - long-chain fatty acid
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent acyl-CoA ligase"
                     /protein_id="HLY91368.1"
                     /translation="LGCGWIGAVTVPINVASRGAQLAHILTNSQAKLLVVQADHAEAL
                     ETLDAVPPSLRAIWLIGDAPVAPRWPNVSSFPAPAEPVPEAPVGPGDTVAILYTSGTT
                     GVSKGVCCPHAQYFWWGVNTARLLGVRGDDVLLTTLPLFHTNALNTFYQALLTGATLV
                     VEPRFSASGFAPSLVHHGATVTYLLGAMVPILLSRPSSELDRAHQVRIALAPGVPERF
                     HPEFRDRFGIALLDGYGSTETNFVLGCALQQQRGGAMGPLFTGFDARVVDDADNTLPD
                     GTPGELVLRADEPFAFATGYFGMPEKTVEAWRNLWFHTGDRVVRDVDGYYRFIDRMKD
                     AIRRRGENVSSYEVEQVLLSHPDVSEAAAFPVQSELAEDEVMAAIVPRDGHALTPAML
                     ADYCKGRMPVFAIPRFIEFVTALPMTENGKVQKFRLRERGVSETTWDRQTSVTGWQIT
                     RPPVSA"
     gene            complement(1348..3402)
                     /gene="recG"
                     /locus_tag="VKQ27_20475"
     CDS             complement(1348..3402)
                     /gene="recG"
                     /locus_tag="VKQ27_20475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HLY91369.1"
                     /translation="MDYDPLTPLFSPLTTLKGVGPTVGALIAKVAGGERVVDLLFHLP
                     ESYVDRRQRPTIARAQAGQIATLAVEVVRIEPPANARQPWRVVVTDGTASAELVFFRF
                     NREKQMPVGAKLLVSGKVEGFGGRRVIAHPEHVVPADRPESMPAVEPVWPLTGGLWPR
                     QVGSAMAQALGRVPSLPEWHDAALLRRETWPSFIEALRAVQAPTEVPDQKCRERLAYD
                     ELLADQVALAVVRGRLRTRPGRALAGDGVLRAKALERFGFTLTPSQTTALREIDADLS
                     SERRMLRLLQGDVGSGKTLVALLAMLRVVEAGKQAALMAPTEVLAKQHHRTLSRLCPE
                     PVALLTGGVKGRARSALLRQLKDGAVRMVVGTHALFQETVEYHDLALAVIDEQHRFGV
                     DQRLLLGRKGELTDVLVMTATPIPRTLVLTQWGEMDVSRLTEKPPGRGRVRTSLHSLG
                     TMPELLGAVTRKLDEGAQVYWICPLVAESEVMDLAAAEARFAELRERLGRRVGLAHGR
                     QDPAVRDATLAAFAQGDCRLLVATTVVEVGVDVPTATVMVIEHAERFGLAQLHQLRGR
                     VGRGAAASFCLLLHEEWVNDSAKRRLTLLRDTEDGFVIADEDFRLRGGGDVLGTKQSG
                     LPGFRLADPIEHEGLLHMANRDAAVLLGKDPKLTSERGRAVRVLLRVFEKAAAMQTLA
                     AG"
     gene            3446..3763
                     /locus_tag="VKQ27_20480"
     CDS             3446..3763
                     /locus_tag="VKQ27_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HLY91370.1"
                     /translation="MSEPEPASLDVRRRRLLYRATHRGTFENDLLLGGFVRKHLATLP
                     ESDLTGLEALLDIPDNQLADWLTGREPIPAQAASPMLIRVRASAPTLYREAGEGRDEV
                     RST"
     gene            3760..>5285
                     /locus_tag="VKQ27_20485"
     CDS             3760..>5285
                     /locus_tag="VKQ27_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HLY91371.1"
                     /translation="MTPTAIYGAPEGWDAFLLARRRGEFPGPILHVARDDARMARLAE
                     ALAFVVPEAEILRFPAWDCLPYDRVSPNPALVSERIATLGRLLDAPRGPRIVLTTVNA
                     LVQRVPPRAAFAGASLDLRVNGSIQPENLARFLEANGYGRAGTVMEPGEYAMRGGIID
                     IFPAGEADPVRLDLFGDSIESIRAFDPTTQRSADKRQRLTLRPVSEVPLDPDAISRFR
                     TGWRDLFGQPAATDPIYLSISDGRRHPGMEHWVPLFHPSMETLLDYLPGASVSFDHQA
                     DEVLRARLEMIADHYAARRLAPRDGEVPYRPLPPDRLYLDEAGWNATLSIGPSFAFSP
                     FARPDGAVGVDGGGRPGPVFFQGGGPGINVFDQLRAQSERWSGEKRRIVIAAWTRGSR
                     ERLANLLREHGFKDATQETGWAAIRGKPPGTFSLVTLGVERGFVADGLALVGEQDLLG
                     ERISRPPRRRKRADQFIAEATEIAEGDLVVHQEYGIGRYDGLSTLTVTGAPHDCLRLI
                     "
BASE COUNT          870 a         2013 c         1495 g          907 t
ORIGIN      
        1 atctcggctg cggctggatc ggtgccgtca cggtgccgat caacgtcgcg tcccgaggag
       61 cacagctcgc gcacatcctg accaactcgc aggcgaagct tttggtcgtg caagccgatc
      121 atgccgaggc cctggaaacg ctcgatgctg tccctccctc gctgcgtgcc atatggctga
      181 tcggcgacgc gccagtcgcg ccccgctggc cgaatgtctc cagcttcccg gcaccagccg
      241 aacccgttcc cgaagcccca gtcgggcccg gcgataccgt tgcgatcctc tacacctccg
      301 gcaccaccgg cgtgtccaaa ggtgtttgct gcccgcacgc gcaatacttc tggtggggcg
      361 tcaacaccgc gcgcctgctc ggcgtgcgcg gcgacgatgt actgctgacc acgctgccgc
      421 tgttccacac caacgcgctg aacacgttct accaggcgct gctgaccggt gcgacactgg
      481 tggttgaacc gcgcttctct gcctcggggt ttgcgccgtc gctcgtccat cacggtgcca
      541 ccgttacgta tctgctcggt gccatggtgc cgatactgtt gtcacggccg tccagcgaac
      601 tggatcgcgc acaccaggtg cgcattgcgc tggcccccgg tgtgccggag cggttccatc
      661 ccgagttccg cgaccgcttc ggcatcgcat tgctggacgg ctacggctcg actgaaacga
      721 atttcgtcct gggctgcgca ctgcagcaac agcgtggcgg cgcgatgggc ccccttttca
      781 cagggtttga cgcccgcgtc gtcgacgacg ccgacaacac attgcccgat gggacgcccg
      841 gcgaactggt gcttcgcgcc gacgaacctt tcgccttcgc caccgggtat ttcggcatgc
      901 ctgagaagac cgtcgaagcc tggcgcaacc tgtggttcca tactggcgac cgcgtggtgc
      961 gcgatgtcga cggctattat cgcttcatcg accgcatgaa ggacgcgatc cgccggcgtg
     1021 gcgaaaatgt ctcctcctat gaggttgaac aggtccttct gtctcatccg gatgtctcgg
     1081 aagcggcagc ctttccggtg cagtccgaac tggccgagga tgaggtcatg gcggcaatcg
     1141 tcccgcgcga tggccatgcg ctgacacccg cgatgttggc tgattactgc aagggccgca
     1201 tgcccgtctt cgctatcccg cgcttcatcg agttcgtgac cgcgctgcct atgaccgaga
     1261 acggcaaggt gcagaaattc cgtctacgcg agcggggcgt ttccgagacg acctgggatc
     1321 ggcagacgtc agtcacagga tggcaaatca cccggccgcc agtgtctgca tagccgcggc
     1381 cttctcgaac acccgcagca gcacgcgtac tgcccgtccg cgctcgctcg tcagcttcgg
     1441 gtccttccct agcagcaccg ccgcatcgcg gttcgccatg tgcagcagcc cttcatgctc
     1501 gatcggatcg gccagccgga acccgggcag acccgattgc ttcgtcccga ggacgtcgcc
     1561 gccgccacgc aaccggaaat cctcgtcggc gatcacgaac ccatcctcgg tatcccgcag
     1621 caacgtcagc cgccgcttcg ccgaatcatt cacccactcc tcatgcagca agagacagaa
     1681 actcgccgcc gcgccgcgtc ccacgcgccc gcgcaactga tgcaactgcg ccagcccgaa
     1741 ccgttcggca tgctcgatca ccatgaccgt ggccgtcggc acatcgaccc ccacctccac
     1801 taccgtcgtc gccaccagca gccggcagtc accttgcgcg aaggccgcca gcgtcgcgtc
     1861 ccgcaccgcc ggatcctgcc gcccatgcgc cagccccacg cgcctaccga gccgctcgcg
     1921 cagctccgcg aaccgggcct cggccgcggc cagatccata acctcgctct ccgccaccag
     1981 cgggcagatc cagtaaacct gtgccccctc atccagcttc cgggtcaccg ctcccagcaa
     2041 ctccggcatc gtccccagcg aatgcagtga cgtgcgcacc cgcccccgtc caggcggctt
     2101 ctcggtcaac cgcgacacgt ccatctcgcc ccactgcgtc agcaccagcg tccgaggtat
     2161 cggcgtcgcc gtcatcacca gcacgtctgt caattcgccc ttccgcccga gcagcagccg
     2221 ctgatcaacc ccgaaccggt gctgctcgtc gatcaccgca agtgccaggt cgtggtactc
     2281 gacggtctcc tggaacagcg catgcgtccc caccaccatg cgaaccgcgc catccttcaa
     2341 ctggcgcagc agcgccgacc gcgctcgccc tttcacacct ccggtcaaca acgccaccgg
     2401 ctccgggcac aaccgcgaca gggtgcgatg atgctgtttc gccaacacct cggtcggcgc
     2461 catcagcgcg gcttgcttgc cggcttccac cacacgcagc atggccagca gggcgaccag
     2521 tgtcttgcct gaccccacat ccccttgcag taaccgcaac atccgccgtt ccgaggacag
     2581 atcggcatcg atctcccgca atgcggtcgt ctgcgacggc gtcagcgtga agccaaaccg
     2641 ctccagcgcc ttggcccgca gcacgccgtc gccagccagc gcgcgtcccg ggcgggtccg
     2701 caaccgtccc cgcaccactg ccaatgccac ctgatccgcc aaaagctcgt catacgccaa
     2761 acgctcccgg catttttggt ccggaacctc ggtgggcgcc tgcacggccc gcaatgcctc
     2821 gatgaagctc ggccaagtct cccgccgcaa cagggccgcg tcgtgccact ccggcagtga
     2881 cggcacccgc cccaacgcct gcgccatcgc gctgccaacc tgccggggcc acaacccgcc
     2941 agtcagcggc cacaccggct ccaccgcagg catcgactcg ggccgatccg ccggcaccac
     3001 gtgctccgga tgcgcgatca cccgccggcc gccgaatccc tccaccttgc cggagacgag
     3061 cagcttggct cccaccggca tctgcttctc acggttgaac ctgaagaaca ccagttcggc
     3121 ggatgcggtc ccatcggtca ccaccacccg ccacggctgc cgggcattgg ccggcggctc
     3181 gatccggacc acctcaactg ccagcgtcgc aatctgccct gcctgcgctc gagcgatcgt
     3241 tggccgctgg cgcctgtcca cgtacgactc gggcaaatgg aacagcaggt cgacgacccg
     3301 ctcaccgcca gccactttgg caatcagcgc gcccaccgtc ggcccgacgc ctttcagcgt
     3361 ggtcagcggc gagaacaatg gtgtcagagg atcataatcc acgggctgac atagggttat
     3421 cgcgcggcta tatgacaccc cgccgatgag tgaaccagaa ccagcctcgc tcgatgtgag
     3481 gcgccgcaga cttctgtatc gcgctaccca ccgcgggacg ttcgaaaatg atctcctgct
     3541 cggtggcttt gtacgaaagc acctggccac attgcccgag tcggacctta ccggcctcga
     3601 agcgctgctc gacatccccg acaaccagct cgccgactgg ctgaccggtc gcgagcccat
     3661 cccggcgcaa gccgcctcgc ccatgctgat tcgcgtccgt gcttccgccc ccacactcta
     3721 tcgcgaagcc ggggagggca gggacgaggt gcgctccaca tgacccccac tgccatctac
     3781 ggagcgcctg aaggctggga tgccttcctc cttgcccgcc gccgcggtga attcccgggc
     3841 ccaattctgc acgttgctcg cgacgacgcg cgcatggcgc gcctcgccga ggccctcgct
     3901 tttgtcgtcc cagaggccga aatcctccgc tttcccgcct gggattgcct gccttacgac
     3961 cgcgtctcgc caaatcccgc gctggtctcc gagcgcatcg ccacgctcgg ccgtctgctg
     4021 gatgcgccaa ggggacctcg cattgtcctc acgaccgtca acgctctagt ccaacgcgtt
     4081 cccccacgcg ctgccttcgc cggtgccagc ctcgatttgc gcgtcaatgg ctctatccag
     4141 ccggaaaacc tggcccgctt cctggaagcc aacggttacg ggcgcgccgg cacggtgatg
     4201 gagccgggtg aatatgccat gcgcggtggc attatcgaca tcttccccgc cggcgaagcg
     4261 gaccccgtcc gcctcgacct gttcggcgac tccatcgagt cgatccgcgc cttcgatccc
     4321 accacccagc gcagcgcgga caagcgccaa cgcctgaccc tgcgccccgt ctctgaagtt
     4381 cccctcgatc ccgacgccat ctcccgattc cgcaccggct ggcgcgacct gttcggccag
     4441 ccagctgcca ccgatccgat ttacctgtcc atttcggatg gccgccgcca ccccggcatg
     4501 gagcattggg tcccgctctt ccatccatcg atggagacac tgctggacta ccttccgggg
     4561 gcttctgtca gctttgatca tcaggccgat gaagtcctgc gcgcccgtct ggagatgatc
     4621 gccgaccatt atgccgcccg ccgcctcgcc ccgcgcgatg gcgaagtccc ctaccgcccg
     4681 cttcctcccg atcgcctgta cctcgacgag gccggttgga atgcgacgct gtccattggc
     4741 ccgtcattcg cattctctcc gttcgcccgc cccgacggcg cggtgggcgt tgacggcggc
     4801 ggccgccccg gcccggtatt tttccagggc ggcggacccg gtatcaacgt gttcgatcag
     4861 ctccgtgccc agtccgaacg atggtccggc gaaaaacgcc ggatcgtcat tgccgcctgg
     4921 acccgcggct cccgcgagcg cctcgccaac ctgctgcgcg agcatggctt caaggacgcc
     4981 acacaggaga ccggctgggc agcgatccgc ggtaagcctc ctggcacttt ctcactcgtc
     5041 acactcggcg tggaacgcgg tttcgttgcc gacgggctcg cgttggtggg cgagcaggac
     5101 ctgttgggcg aacgcatcag ccgcccgcca cgccgccgca agcgcgccga ccagttcatc
     5161 gccgaagcca ccgaaatcgc cgaaggcgac ttggtcgtac accaggaata cggcatcggc
     5221 cgctatgacg gtctctccac tctcacggtc accggcgcgc cgcacgattg cctgcgcctg
     5281 atcta
//
LOCUS       DATGEM010000663         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_48464, whole genome shotgun sequence.
ACCESSION   DATGEM010000663 DATGEM010000000
VERSION     DATGEM010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48464"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VKQ27_20490"
     CDS             complement(<1..336)
                     /locus_tag="VKQ27_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91372.1"
                     /translation="MTILSGWRATTAPRWMMLGEWRAHPVRVLTAIVAIAIGVALGFA
                     VHLVNASALDRFARGLATVNGGADLRIEAAGAGGFDEGLYPRIARLPGIVAASPVIKM
                     HARIDGRPID"
     gene            complement(333..1001)
                     /locus_tag="VKQ27_20495"
     CDS             complement(333..1001)
                     /locus_tag="VKQ27_20495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91373.1"
                     /translation="MTPLLAFSGLRKAVPSGRLLFDRVSLDLERGELVAIMGESGVGK
                     STLLNIVAGLDTPDNGAVTLDGMDLALLDEDGRAALRRRHVGFIFQAFHILPHLTLGQ
                     NVALPLALLHAAPAEAVARSDDLLAAVGLAGRAGDYPSQLSGGELQRVAIARAIAHRP
                     SLILADEPTGNLDPETAARVLTLLVKQLRDAGAGALLVTHSEVAAASADRALRLTTAG
                     LVPA"
     gene            complement(1342..2334)
                     /locus_tag="VKQ27_20500"
     CDS             complement(1342..2334)
                     /locus_tag="VKQ27_20500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLY91374.1"
                     /translation="MGPILSRRFKAYGADWKRSLFQLVTTAMPFIALLVIMPEASHVS
                     YWLTLLLAIPAAGLLVRLFIIQHDCGHGSFFKSRAANDGLGRVLGVLTLTPYGHWRKG
                     HAVHHACSGSLDRRGRGDVEMLTVAEYEALSPLGKLGYRLYRNPFIMVLLGAPINFIV
                     LQRVPRGLSFRDRDARRSILSLNVALVVAFGLPMVMIGVAPVVATYLPVMFIASWAGN
                     WLFYVQHQFENTYWKREDDWNFYTAALRGSSYFELPRILQWFSGNIGLHHVHHLCSRV
                     PNYRLQACLDSAPELHRIAKRITLRESLGCWKLALWDEERRLMVGFSDLKFRSA"
     gene            complement(3014..3391)
                     /locus_tag="VKQ27_20505"
     CDS             complement(3014..3391)
                     /locus_tag="VKQ27_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91375.1"
                     /translation="MTRNQPPRGSPATCHPRYRSVSTNSLNADDACRRLGYAPMRDQP
                     IGRSDDRTIASWPTGAVVSETFPPQAIGCKPSDKKAYNSLYIRLFRLPRQEGLDLTDA
                     LFSCNELDEPLIDFFAKLGGYKR"
     gene            complement(3508..4989)
                     /locus_tag="VKQ27_20510"
     CDS             complement(3508..4989)
                     /locus_tag="VKQ27_20510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY91376.1"
                     /translation="MLLFATALAGLAVAGAHAASILPPPPAELDAAVKAYVRVPAGRI
                     ALSHVRLIDGTGAAAQDDRTILLNGARIEAVLPGNATIPKGVTVLDLNGETVLPGLVG
                     MHDHMYYIGRPDLDAQGHSEPPVVVPQMPFTSPRLYLAAGVTTLRTTGSVEPYTDLNL
                     KKQIDAGQLPGPHLDVTGPYLEGPGSPFIQMHQLADAEDARRTVDYWADQGATSFKAY
                     MNITRAELGAAIDEAHKRGFKITGHLCAVTYPEAAALGIDDLEHGFFVNTQNDPGKQP
                     DTCPATTGTPTLAAMTPDSPDAKALIATLVTHHVAVTSTLPVFEQQVPGHAPLWPQAM
                     ATLTPEAREAYLYLRDRRSNAQRDLASTSAAVTAFHNDLGLERAFVAAGGLLLAGPDP
                     TGNGGVIPGFGDQREIELLVEAGFTPEQAVRIATLNGAIYLGLQDRIGTVAAGKNADL
                     LIVRGDPAHQITDIENADIVFKDGVGYDTARLLASVRGRYGEY"
     gene            4939..5970
                     /locus_tag="VKQ27_20515"
     CDS             4939..5970
                     /locus_tag="VKQ27_20515"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91377.1"
                     /translation="MGPGDRQPRECSCEQQHSSSHVASLTLVAHCSPWVGDDAVFPES
                     EQSRTPGAALFDQRPTCVDDGLKAILSRRPSLLMVPRIAGDDAMKSIRLTALAGVLLL
                     AVFAVPASAATAPLDMSAAVARPGRPAEAVVMDAGRKPAEVLSFLGLRPGMAAADIMT
                     GSGYWAEIMANEVGAKGQVTAFEPQQFYTTPDEQKTWSAMLARRPDIHFMRYPFESFA
                     SAPRSFDFAMISLNYHDLYWESVKYKIPHTDPAAFLHTLYAAMKPGGIVGVIDHVGPA
                     GDTRAIVDKLHRIDPETVKADFKAAGFVLEAESPLLANPADDHSKLVFDPLIRGKTDR
                     FVYRFRKPR"
     gene            6154..>6624
                     /locus_tag="VKQ27_20520"
     CDS             6154..>6624
                     /locus_tag="VKQ27_20520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLY91378.1"
                     /translation="MTDMAANVERRRVAVEEADLHRALKRFVGSRLRDRAEGEDIVQE
                     TYLRLYTYRRTRTVADVGAFCFAVARNLVQDHLRRLKRAPLMGELDMEAPCPAPRADE
                     ILIHRQRVAVLTHALGVMPPLRREVFLRRRLDEQPIARIAEDLELSAAAVEKHVT"
BASE COUNT         1196 a         2048 c         2215 g         1165 t
ORIGIN      
        1 gtcgatcggg cgcccgtcga tccgggcatg catcttgatg accgggctgg ctgcgacgat
       61 tccgggcagt cgggcgatac gcgggtagag gccctcgtcg aagccacctg cgcctgccgc
      121 ctcgatccgg agatccgcgc caccattcac ggtggcaagg ccgcgcgcga agcggtcgag
      181 cgcggaagcg ttgacgagat gcaccgcaaa gcccaatgcc acgccgatcg cgatagccac
      241 gatcgctgtc aggacgcgca ctggatgcgc tcgccactcg cccagcatca tccagcgtgg
      301 cgcggtcgtc gcccgccagc cggataggat ggtcacgccg gcaccagccc ggcagtggta
      361 aggcgcagcg cgcgatcggc gctcgcggcg gcaacctcgg aatgggtgac gagcagggcg
      421 ccggcgccag cgtctcgcag ctgcttcacc aacaaggtca gcacgcgcgc agctgtctcg
      481 ggatcgagat tgcccgttgg ctcgtcggca aggatcagcg acggtcggtg agcgattgca
      541 cgggcaatcg cgacgcgctg caattctccg cccgaaagct gactcggata gtcgccggcg
      601 cgcccggcga ggccgactgc ggcgaggagg tcgtccgatc gtgccactgc ctccgctgga
      661 gctgcatgca gcaatgccag gggtaaagcg acgttctggc ccagcgtcag atgcggcagg
      721 atgtgaaagg cttgaaagat gaagccgaca tggcggcggc gaagggcggc gcggccatct
      781 tcgtcgagca gggcgagatc catcccgtcg agcgtcaccg caccgttatc cggcgtgtcg
      841 agtccggcga cgatgttgag cagggtcgac ttgccgaccc cagactctcc catgatcgcc
      901 accagttcgc cccgttccag atcgagcgac acgcggtcga acagcaatct gccgctcggc
      961 acagccttgc ggaggccgga gaatgccagg agcggtgtca tggccatgac acctgtctag
     1021 tgtcctgccg aaggtttaca aagtgacatc ggcaacgcgc cagccatgcg catggcattg
     1081 cgttgcgggt catgcgcgac cgtcgcgctg cccgcttagc gcccaacccc agacatacgg
     1141 gagcttggaa gtgcgtcccg aaaaccgcca gaccgttttt taagcgcaag caccggcgcc
     1201 ctttggagga ttggcatcga cgcgcgtagc ggtcgcggtt agccccgtcg tcgctccggc
     1261 gcgtaattcc cgccgctccc atcggcgtag gtgcagaggg aagcaattcg cgcctgcaaa
     1321 agagccggcc taacgtagcg tctatgcgct ccgaaacttc aaatcgctga accccaccat
     1381 tagacggcgc tcttcgtccc atagcgccag tttccagcat cccaagctct cgcgcagcgt
     1441 tatccgtttt gcgatgcggt gtaattccgg agcgctgtcg agacaggcct gcaggcggta
     1501 attgggaaca cggctgcaca ggtgatgaac gtgatgcagg ccgatattcc cgctgaacca
     1561 ctgcaagata cgtggaagtt cgaaatagga actgccgcga agggccgctg tatagaaatt
     1621 ccagtcgtct tcccgtttcc aataggtgtt ttcgaactgg tgctggacgt aaaacagcca
     1681 attgccggcc caggacgcga tgaacatcac cggcagatag gtcgcaacga ccggggcaac
     1741 gccgatcatc accatcggca agccgaaagc cacgaccagg gcgacgttca gcgacaggat
     1801 actgcgacgt gcgtcgcggt cccggaacga cagccctctc ggcaccctct gaagcacgat
     1861 gaagttgatc ggtgcgccca gcaggaccat gatgaaagga ttgcgataca ggcggtagcc
     1921 tagctttccc agcggggaca gggcttcgta ctcggcgacc gtcagcattt cgacgtcgcc
     1981 acggccgcga cggtcgagac tgccgctgca tgcatgatgc acggcgtgac cttttctcca
     2041 atggccatag ggggtgagtg tcagcacacc aagcacacgg ccgagaccat cattggcggc
     2101 ccgcgacttg aagaatgagc cgtgaccgca gtcatgctgg atgatgaaga ggcgaacgag
     2161 cagcccggcc gccgggatcg cgagcagaag cgtcagccaa tagctgacat gtgatgcctc
     2221 aggcatgatg acgagcaagg cgatgaatgg catcgcggtc gtcaccaact ggaacaggct
     2281 tcgtttccaa tcggcgccat aggctttgaa ccgccgcgat aggatggggc ccatcacctc
     2341 gacctccgaa gagagcacac tgcaatcgct cggttcgctt cccattgggg gtgagccttg
     2401 gccgacaact tgctcgggac gtgttttgac cggtactgac gtcaatgatc acctgctctc
     2461 aaccctctcc gcgcggggaa aacccggcgg tagagaccga ggccgcgttt ctggctccga
     2521 tggaaaggag gaacagtgcc gcaatgaaga cgcttgcgtc taccatcatc gcctcgggcc
     2581 tgggtggatt agcgggagtg gccgcagata gcgaatctca aatacgcccg actatgctgc
     2641 gagattgggt atgccggggc ccattcggtg ttcgtgacaa ttggacgcag tcgggcatca
     2701 aacgatccgt cgcggacggc atcttccggt ccctgtttcg aaagcggcct aggcggagtg
     2761 gacgaattgg ttgacgcagc gcgagcgggt tgattcaagg ctgccttttg gaggccggct
     2821 tgagccgggg tgatctgagc gaagcggaat ggcgtgtttt gaagggcttg ctgccgatcg
     2881 atgcagcgaa ccgggggcgc ggccgccgtc cagagcagaa ccgctctatc atgaacggca
     2941 tattttggcg gcttcgctgc gggacgcctt ggcgtgatgt tccggccaac tttcgccgcg
     3001 aggtcgaggg caatcagcgc ttatagccgc ccagcttggc aaagaaatcg atcagcggct
     3061 catccagttc gttgcaagag aatagggcat cggtcagatc gagcccctcc tgacgcggca
     3121 gtcggaacag gcggatgtaa agcgaattat acgctttctt gtcagacggt ttgcatccga
     3181 tcgcctgagg cggaaatgtc tcggagacga ctgctcctgt gggccaactc gcgatcgtcc
     3241 gatcgtccga tcgtccgatc ggctgatcgc gcatcggcgc gtatcccaac cggcggcacg
     3301 catcgtccgc gttcaggctg ttggtggaga cgctgcggta tcgtgggtga caagtcgccg
     3361 gcgagccgcg cggcggctga ttgcgagtca tgccgatgct catgtccggg ggccgtttgc
     3421 ggaggtagca cgtggccagt caggcggtac tgcttcgcgc ctgtttggct cccgcaagcc
     3481 ttcatttgtc cggtgcattg cgaggcctca atattcaccg taacggccac ggaccgaggc
     3541 gagcagccgc gcggtatcat agccgacgcc atccttgaac acgatgtcgg cattctcgat
     3601 gtcggtgatc tggtgggcgg gatcgccgcg cacgatcagc aggtcggcat tcttgccggc
     3661 ggcaaccgtt ccgatgcgat cctgaagccc gagatagatg gcgccgttga gcgtcgcgat
     3721 gcggaccgcc tgctcgggcg tgaagccagc ctcgaccaga agctcgatct cgcgctggtc
     3781 gccgaagccg gggatgacgc cgccattgcc ggtcgggtcc ggcccggcca gcagcagtcc
     3841 gccggcagcg acgaaggcgc gctccagccc gagatcgtta tggaaggcgg tgacggcggc
     3901 tgaggtggag gccaaatccc gctgcgcgtt ggagcggcgg tcgcgtaggt agagataggc
     3961 ctcgcgcgcc tcgggagtca gcgtcgccat cgcctgcggc cagagcggcg catggcccgg
     4021 cacctgctgc tcgaacaccg gcagcgtcga ggtgacggca acatgatgcg tgaccagcgt
     4081 cgcgatcagc gccttcgcgt ccgggctatc cggcgtcatc gcggcgagcg ttggcgtgcc
     4141 ggtggtcgcg gggcatgtgt cgggctgctt gccgggatca ttctgcgtgt tgacgaagaa
     4201 gccatgttcc agatcgtcga tgccgagcgc ggcggcctcg ggataggtga cggcgcagag
     4261 atggccggtg atcttgaagc cgcgcttgtg cgcctcgtcg atcgccgcgc cgagctcggc
     4321 gcgggtgatg ttcatatagg ccttgaacga ggtggcgcct tggtcggccc aatagtcgac
     4381 ggtgcggcgc gcatcctcgg cgtcggcgag ctgatgcatc tggatgaacg ggctgccggg
     4441 gccttcgaga taggggccgg tgacgtctaa gtgcgggccg gggagctgcc cggcgtcgat
     4501 ctgcttcttg aggttgagat cggtataggg ctcgacgctg cccgtcgtcc gcagcgtggt
     4561 gacgccggcc gcgagataga ggcgcggcga ggtgaagggc atctgcggca cgaccaccgg
     4621 gggctcactg tggccctgcg catcgaggtc gggccggccg atataataca tatggtcgtg
     4681 catgccgacg aggccgggca gcaccgtctc gccgttgaga tcgagcaccg tcacgccttt
     4741 ggggatcgtg gcgttgccgg gcagcacggc ctcgatgcgg gcgccgttga ggaggatggt
     4801 gcgatcgtcc tgcgccgctg cgccggtgcc atcgatcagg cggacgtggc tcagcgcgat
     4861 ccggccggcg ggtacgcgca cataggcctt cacggcggca tcaagctcag ccggaggcgg
     4921 cgggaggatc gaggcggcgt gggccccggc gaccgccagc cccgcgagtg cagttgcgaa
     4981 cagcagcatt cgtccagtca tgtagcctcc ctcacgctcg tggcgcattg ctcgccttgg
     5041 gtaggcgatg atgcagtctt tccggaatcg gaacaatcgc ggacgccggg ggccgcgctt
     5101 ttcgatcaac gcccaacatg tgtcgacgac ggtctgaaag ccatattgag tcgccggccg
     5161 agcctgctca tggttccacg catagcaggg gatgatgcca tgaaatccat tcgtctgacg
     5221 gcgctcgcgg gcgtccttct tcttgccgtg tttgccgtgc cggccagcgc cgcgacggct
     5281 ccgctcgata tgtcggccgc ggtcgccagg ccggggcgcc ccgccgaggc ggtggtgatg
     5341 gacgccggcc gcaaaccggc agaggttctg tccttcctcg gtctgcgtcc agggatggcg
     5401 gccgccgaca tcatgacggg ctcgggctat tgggccgaga tcatggccaa tgaagtgggc
     5461 gccaaggggc aggtgaccgc gttcgagcca cagcaattct acactacgcc ggacgagcag
     5521 aagacctggt cggcgatgct cgcgcggcgg ccggacatcc acttcatgcg ctatccgttc
     5581 gagtccttcg cgagcgcgcc gcgcagcttc gacttcgcga tgatcagcct caactatcac
     5641 gatctctact gggaatcggt caagtacaag atcccgcaca ccgacccggc tgccttcctc
     5701 cacacgctct acgcggcgat gaagccaggc ggcattgttg gcgtgatcga ccatgtcggc
     5761 ccggcgggtg acacgcgcgc gatcgtcgac aagcttcacc gcatcgatcc cgaaacggtg
     5821 aaggcggact tcaaggcggc gggcttcgtg ctcgaagcgg aaagcccgct gctcgccaat
     5881 ccggcggacg atcacagcaa gctggtcttc gatcccttga tccggggcaa gaccgaccgc
     5941 ttcgtctatc gtttccgcaa accccgctga ggtcaccggg gcggggaggc cgagccttca
     6001 cgccccctga tccaattgca tggcgcccaa tgccgccccg gcgcggctgt catgcgcgtt
     6061 tcacacgcaa aatctagaag cgcagcaaga ttggtcatca aaggggggcc tcgccggcgt
     6121 cggcactcgg gtcggcgttg ccgttgcacg tgcatgactg acatggccgc caatgtcgaa
     6181 cgccgtcggg tggccgtgga agaggccgat ctccatcggg cgctgaaacg gttcgtcggg
     6241 agccggctgc gcgatcgcgc ggagggcgag gacattgtcc aggaaaccta tctccgcctc
     6301 tatacctatc gccgcacgcg tacggtcgcc gatgtagggg cgttctgctt tgcggttgcg
     6361 cgcaacctgg tgcaggatca tctccgccgc ttgaagcgcg cgccgctgat gggcgagctg
     6421 gacatggaag caccatgccc cgccccgcgc gccgacgaaa ttctgatcca ccggcagcgg
     6481 gtggccgtgc tgacgcatgc gctcggcgtt atgccgccct tgcgccgaga ggttttcctg
     6541 cgccgccgcc tcgacgagca gccgatcgcc cggatcgccg aggatctgga actgagcgcc
     6601 gcggcagtcg aaaagcatgt gaca
//
LOCUS       DATGEM010000664         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_761508, whole genome shotgun sequence.
ACCESSION   DATGEM010000664 DATGEM010000000
VERSION     DATGEM010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761508"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VKQ27_20525"
     CDS             <1..708
                     /locus_tag="VKQ27_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY91379.1"
                     /translation="GGGLNSTIAGLASQSHPFNPFDEFKDEFRVISADLRNAKGGQST
                     GPLETDRPWDAYTDDHLGLMDHLGIRKFLVLGFCIGGPFIWNMLQRAGDRVIAAIPAQ
                     PSGWRPEMPTLSYDNNMKTWGPELCARRPDITMAMVDTFLTNMYRNRGDFVYTVSRDF
                     VRGCQTPILVLPDDVPAHPYAVAMETVRLAPNAQVSLYPWKDTPDKIPLAVRHIRTFL
                     RAHRPAAETRTMAAAAQ"
     gene            766..1545
                     /locus_tag="VKQ27_20530"
     CDS             766..1545
                     /locus_tag="VKQ27_20530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY91380.1"
                     /translation="MGRLDGKIAAVTGGASGIGEATVRRFVAEGASVAFCDRDGERGE
                     RVAAELEAAGAKVAFTEADVATESACLGFVNSAAEKFGRLNILVNNAGIRKYEKIDEA
                     SAASWTEILSVNLMSYAFCAKAAVPLMRRNQGGAPGGAIVNVASVRSVVAGGGNLQYD
                     TTKAAIAGLTRGLAADHSVEGIRVNAVGPGPIFTPFHARRIAAAGETVEQYNAKAAQG
                     TMMKRPGRAEEVAAAILFLASDDASYVTGALLFVDGGMTAM"
     gene            complement(1950..>2365)
                     /locus_tag="VKQ27_20535"
     CDS             complement(1950..>2365)
                     /locus_tag="VKQ27_20535"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLY91381.1"
                     /translation="DLRVELIEQPLPADADEALARLQWPIPICADESCRTVADLDRLE
                     GKYQAINIKLDKAGGLTEALALAAEAKRRGFRIVVGGSIGTSLGIAPALLVAQQAHIV
                     DLDGPLHLAVDRGAQLRYDGSTIHPADPKLWGGPG"
BASE COUNT          431 a          824 c          717 g          393 t
ORIGIN      
        1 ggcggtggcc tgaactcaac gatcgcgggc ctggcgagcc aatcccaccc gttcaatccg
       61 ttcgacgaat tcaaggacga attccgcgtg atctcggcgg atctgcgcaa cgccaagggc
      121 ggccaatcca caggcccgct cgagaccgac cgcccatggg acgcctatac cgacgaccat
      181 ctcggcctga tggatcacct cggcatccgg aagttccttg tgctcggctt ctgcatcggc
      241 ggccccttca tctggaacat gctgcagcgc gcgggcgacc gcgtcatcgc ggcgataccg
      301 gcacagccca gcggctggcg ccccgagatg cccaccctct cctacgacaa caacatgaag
      361 acctggggtc cggaactctg cgctcggcgc cccgatatca cgatggcgat ggtcgacacc
      421 ttcctgacca acatgtaccg caaccgcggc gacttcgttt acaccgtatc ccgcgatttc
      481 gtccgcggct gccagacgcc gatactggta ctgccggacg acgtgccggc gcatccatac
      541 gccgtcgcca tggaaaccgt gcgtctcgcg ccgaatgcgc aggtgagcct ttatccgtgg
      601 aaggacacgc cggataagat cccgctggcg gtgcgccata tccgcacatt cctgcgcgca
      661 catcggccag cggcggaaac ccggaccatg gcggccgccg cccagtagcg tcacatcgct
      721 gccgctgtag acacacgctc cgcaatagaa gggaaggaga caaccatggg aaggctcgac
      781 ggcaagatcg cagcggtgac aggcggcgca tcgggtatcg gcgaagcgac ggtcaggcgc
      841 ttcgtcgccg agggcgccag cgtggcattc tgcgatcgtg acggcgagcg cggcgagcgc
      901 gtggcggccg aacttgaggc cgccggcgcc aaggtcgcct tcaccgaggc cgacgttgcc
      961 accgagtccg catgcctcgg cttcgtcaac agtgcggcgg aaaaattcgg acgcctcaac
     1021 atcctggtga acaatgccgg catccgtaaa tacgagaaaa tagacgaggc cagcgccgcg
     1081 agctggaccg agatcctcag cgtcaacctg atgagctacg ccttctgtgc caaggcggcg
     1141 gtcccgctga tgcgccgcaa ccagggcggc gcccccgggg gggccatcgt caacgtcgcc
     1201 tccgttcgtt ccgttgtcgc cggcggcggc aacctgcaat acgacaccac caaggccgcg
     1261 atcgccggcc tgacgcgagg gctcgcagcc gatcattcgg tcgagggcat tcgggtgaac
     1321 gccgtcggcc ctggcccgat cttcacgccg tttcacgcgc gccgcattgc cgccgccggt
     1381 gaaaccgtcg agcagtacaa tgccaaggcc gcgcaaggca cgatgatgaa gcgcccaggc
     1441 agagccgagg aggtcgccgc cgcgatcctg ttccttgcct ccgacgatgc ctcctatgtc
     1501 accggcgcct tgctgttcgt ggacggcggc atgaccgcga tgtgaggcat gtccacccgg
     1561 cgaggcggcc ctcgccggcg gcatcgcctt ccgtggcata gattgccgtt tcggcgggat
     1621 catcagccat gccagatagg caggctcgtg caggttggcc ggcagagcct gtgactggcc
     1681 atttctcagg tcacacgcaa tgatccgtcc tgctctggca ttcggtgtcc gctcgctgat
     1741 gcaggcagtc ctattatccg aactcagcaa cagccccatg gcgttgaagc ccagttcgca
     1801 actccgtctg cgatccgttt cgcctgtcga ttttcaaccg ccggccggat ggcgtgcatt
     1861 cgacggcgca tgctgcatta tgcgcctcat cgaggatcat ccgctgcggc gcccggccag
     1921 aacagaccgc ggtagccggc gccccaatat taaccaggtc caccccacag cttcggatca
     1981 gccggatgga tcgtgctgcc gtcgtagcgc agctgcgcac cgcgatcgac cgccaggtgc
     2041 agcggcccat ccagatcgac gatgtgcgcc tgctgtgcga cgagaagcgc cggcgcgatc
     2101 ccaagcgacg tgccgatact gccaccgacc acgatccgaa acccgcgccg cttcgcttct
     2161 gccgccagcg cgagcgcctc ggtcagcccg cccgccttat cgagcttgat gttgatcgcc
     2221 tggtatttcc cctcgagccg atcgagatca gcaaccgttc ggcatgattc atcggcacag
     2281 atcggtatcg gccattgcag ccgcgccagc gcctcatcag cgtcagccgg cagcggctgc
     2341 tctatcaact cgacccgcag atcac
//
LOCUS       DATGEM010000665         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1467395, whole genome shotgun sequence.
ACCESSION   DATGEM010000665 DATGEM010000000
VERSION     DATGEM010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467395"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..676
                     /locus_tag="VKQ27_20540"
     CDS             95..676
                     /locus_tag="VKQ27_20540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007400747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HLY91382.1"
                     /translation="MDAERPFAPARVPTDSPEWDAIAAVTRRAMRLTAELNKLPFDDV
                     ARVREIFSQLTGRTVDDTFTLIPPIYTSYGLDIRIGRRVFINQCCTLYDMGGVAIGDL
                     VMIGPNVNIITSGHALSPSQRRTYIEAKPIVIEKNVWIATGATIIGGVTVGENSVVAA
                     GAVVTKDVPPNSLVAGVPAKVIRSLQESPSASP"
     gene            835..1374
                     /locus_tag="VKQ27_20545"
     CDS             835..1374
                     /locus_tag="VKQ27_20545"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLY91383.1"
                     /translation="MRMIVPALSLALTVSAQAQSPWGNAPYQPPYPPAPPTTQPASPT
                     SLAREMLDGHNAVRARVGVPPLGWSASLAQTAQDWADYLVATRAFFHSPDNQDGENLY
                     AISGGSASPNDVVSAWAQEASSYDISRDTCSGVCGHYTQLVWRDTREVGCAVATDTQR
                     EVWVCEYYPPGNIVGYRPY"
     gene            complement(1383..2150)
                     /locus_tag="VKQ27_20550"
     CDS             complement(1383..2150)
                     /locus_tag="VKQ27_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLY91384.1"
                     /translation="MEVRTALITGASSGIGAALAEALAQPGVALFLSGRDAARLEVAA
                     EACRQLGAVAHTTVLDVRDAEAMTGWIRGAGRLDLVVANAGVSGRSREGGPEDAAKTR
                     AIFDINLDGVLNTVLPALEMMVAQTPGADGVRGRIVAVSSLAAFMAAPDSAAYCASKA
                     AVHGWMIARALKARKQGVLLTSVCPGFIRTPMTAGYRFPMPGLMDAEPAARVILRGVA
                     AGRLTVVFPRWMGLAARVAGMLPPRLMAAVVVRRTNT"
     gene            2176..3054
                     /locus_tag="VKQ27_20555"
     CDS             2176..3054
                     /locus_tag="VKQ27_20555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLY91385.1"
                     /translation="MSTPLASMTGFARTEGSDGGLAWVWELRSVNGRGLDLRFRLPSG
                     WDALEPTLREAAGKSLKRGNVTANLSVKRENEPRLVPDPAALEQALTLAMDLHARIPG
                     SPAPRAESLLALPGVLRPAHADDAMDTAPTAAGFSDALAALVTARQAEGTRLRTTLTK
                     LLDEIATLRQRAADEAADQPAAQRARVMENLTNLLREAPSLPEERIAQEVALLAARSD
                     VREELDRLSAHIDAAHALLREGANIGRRFDFLVQEFNREANTLCSKSASVALTATGLK
                     LKATIEQLREQVQNIE"
     gene            3051..3704
                     /gene="gmk"
                     /locus_tag="VKQ27_20560"
     CDS             3051..3704
                     /gene="gmk"
                     /locus_tag="VKQ27_20560"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLY91386.1"
                     /translation="MMRIPRRGICLVLSAPSGAGKTAIADALLSTETDLHRSVSVTTR
                     APRPNEVDGTHYHFLDQAGFDRMAAAGELLEWAHVLGRHCYGTPRAPVEQALSNGQDV
                     VFDIDWQGHNQLRAALPRDVVGVFVLPPSLAALEARLHARSGDPAAEIARRMKLAHDE
                     ISHWAEFDHVVVNTDLAHAIAEVRAILHAARLATGRQTGLTDFVRGLIAPADRTPPR"
     gene            complement(3649..4473)
                     /gene="rsmA"
                     /locus_tag="VKQ27_20565"
     CDS             complement(3649..4473)
                     /gene="rsmA"
                     /locus_tag="VKQ27_20565"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLY91387.1"
                     /translation="MSDQPKLREVIARHGLAAKRSFGQHFLLDANLTRRIVREAGPLA
                     GRHVVEVGPGPGGLTRALLDTEAVSVTVVEIDTRAVAAMQELAAETGDRMRIVAGDAL
                     AMDVEALVPSPRQIVANLPYNVATPLLVGWLRQAAAFERMTLMFQQEVAERICAAPGT
                     AAYGRLSILVQWTCDAALLLRIPPSAFVPPPKVFSAVVGLVPRPAQPEAALFKVMERV
                     TAAAFGQRRKMLRSAMRELGGEALLQEAEIAADRRAETLSVAEFDRLARSVRERNR"
     gene            complement(4470..5777)
                     /locus_tag="VKQ27_20570"
     CDS             complement(4470..5777)
                     /locus_tag="VKQ27_20570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLY91388.1"
                     /translation="MPNRIWRAAALALLLVTPAAAVPPAKPPPPAGQETTRIVAVVNG
                     DVISNADVDNRARLFAVSTGLPLNQDVIGRLKQQITRQLIDERLRMQEVLRLKVVIPD
                     KSIADAIKGIETRNGLPPGGLRQKLLADGVSQRTLIDQIRTQLGWGEVLRGELGDKVK
                     VSDAEVAEQQRLLAQQVGKPEYRLGEIFIPVEDPAVTADAQRFAETVITELRAGAAFP
                     VVAAQFSQNQTALQGGALGWVQPNQLDSAVAQLVAEMPVGAISNPIRVPGGFSIVTVQ
                     GKRQIGNDVVTAISLRQVFLPFTAALNPQAPTEQQKQTLEKAHSISATVHSCDAMEQA
                     AKTYGSTRPADPGEVRLDSINPPALRQVLGSLPLDKATQPLVTNDGIAVLIVCSRGDK
                     NMAQESKEDVRAQLLDERIELLSRQLQADLRRHATIEIRSGGA"
BASE COUNT         1042 a         2267 c         1654 g          940 t
ORIGIN      
        1 ggtacaacct caacggcgac ggcgccgtgg ttgcgaatcg cgaccgatgg cactcctttg
       61 ccgtcacctg catctgaact tgaggcaaac ctccatggat gccgaacggc cgttcgcccc
      121 cgcacgcgtg ccaacagaca gccccgaatg ggatgcaatc gctgctgtga ccagacgggc
      181 catgcggctg acggccgagc tcaacaagct ccccttcgac gatgtcgcca gagtgcgaga
      241 gatcttcagc caactgaccg gccgcactgt agatgacacg ttcaccctca ttccacccat
      301 ttacacctcg tacggtctgg atatccgcat cggccgccgg gtcttcatca accaatgctg
      361 cacgctctat gacatgggcg gtgtcgccat cggcgatctg gtcatgatcg gccccaacgt
      421 gaacatcatc acctcgggcc atgcgctgtc gccatcccag cgacgtacct atatcgaggc
      481 caagccaatc gtcattgaaa agaacgtatg gatcgccacc ggtgccacca tcatcggcgg
      541 cgtcacggtt ggcgaaaatt ctgtggtcgc cgccggagcc gtcgtaacca aagacgtccc
      601 cccgaacagc ctcgtagccg gcgtccccgc caaagtcatt cgctcactcc aggaaagccc
      661 cagcgcttcg ccgtgaaacc ggacctcgat gaggactacc aatcggtggt tcgggtcatg
      721 ccgacgaagg tcggcatcca cgtcgctcgc accgcctagc aggcgccacc ttcttcaagc
      781 ataacgacca gcccgacgcc caagcgtcgc cgtaatccgt ttcgacaata cagcatgcgt
      841 atgatcgtcc cggctctcag cctcgctctc accgtcagtg cccaagccca gtccccctgg
      901 ggcaacgcgc cctaccaacc accatatccg cccgcgccgc cgaccacgca gcctgcctct
      961 cccacatccc tcgcgcggga gatgctcgac ggccacaacg ctgtccgggc ccgtgtcggc
     1021 gtgcctcccc tgggttggtc cgcctcgctc gcccagaccg cccaggactg ggccgattat
     1081 ctcgtcgcca cgcgggcctt cttccacagc cccgacaacc aggacggcga aaacctttat
     1141 gccatctccg ggggctcggc ctcgccgaac gatgtggtgt cggcctgggc gcaggaggcc
     1201 agcagctacg acataagccg agatacgtgt agtggcgtct gtggtcacta cacgcagttg
     1261 gtctggcgcg acacgcgtga ggtcggctgc gcagtcgcga ccgatacgca acgcgaggtc
     1321 tgggtctgcg aatactatcc gcccggcaac attgtcggct accgccccta ctgaggcggc
     1381 gcctacgtat tggtccgccg caccaccacc gccgccatca gccggggcgg cagcattccg
     1441 gcaacccgcg ccgccagccc catccaccgc ggaaacacca ccgtcagccg ccccgccgct
     1501 acgccgcgca ggatgacccg cgccgcgggc tccgcatcca tcagccccgg catgggaaac
     1561 cggtagcccg ccgtcatcgg cgtccggata aaccctggac agacgctggt cagcagcacc
     1621 ccctgcttcc gagccttgag cgcgcgggca atcatccacc catgcaccgc cgccttcgaa
     1681 gcgcagtagg ccgccgaatc cggcgccgcc atgaaagccg ccagcgacga cacagcgacg
     1741 atccgtccgc gcacgccatc cgcccccggt gtttgcgcga ccatcatctc caacgcgggc
     1801 agcacggtat tcagtacccc atccaggttg atatcgaaga tagcgcgcgt cttcgccgca
     1861 tcctccgggc cgccttcgcg cgaccgtccg gaaactccgg cattagcaac gaccaggtcg
     1921 agccgccccg ccccgcgtat ccaccccgtc atcgcctcgg catcgcgcac atccagcacc
     1981 gtcgtatgcg ccaccgcccc aagctgccgg cacgcctccg ccgccacttc aagccgcgcc
     2041 gcatcgcgcc ccgacagaaa cagcgccacc ccaggctgcg ccagtgcctc cgccaaagca
     2101 gcgccaatgc cggatgaggc gccggtgatc agggctgtgc gaacttccat gaaccgagga
     2161 tataacatcg cccgcatgag cacccccctc gcctcgatga caggattcgc ccgcaccgag
     2221 ggcagtgatg gcggcctcgc ctgggtctgg gaactccgca gcgtcaacgg tcgaggcctc
     2281 gaccttcgct tccgccttcc ctcaggctgg gacgcgctgg aacccacact ccgcgaggcc
     2341 gccggcaagt cgctgaagcg cggcaacgtc accgccaatc tgtcagtcaa gcgcgagaac
     2401 gaacctcgcc tcgtccccga ccctgccgcc ctggaacaag ccctgaccct tgcgatggac
     2461 ctccacgccc gcatccccgg cagcccagca ccccgcgcag agtcactgct tgccctgccc
     2521 ggcgtccttc gccccgccca cgcagatgac gcgatggaca ccgccccaac cgccgccggg
     2581 ttcagcgatg cactcgccgc cctggtcacc gcgcgccagg ccgaaggcac ccgcctccgc
     2641 acaaccctga cgaagctgct cgacgagatc gccacccttc gccagcgcgc cgcagacgaa
     2701 gcggccgatc agcccgccgc gcagcgtgcc cgcgtgatgg agaacctgac caacctgctc
     2761 cgcgaggcac cctcgctccc cgaggaacgc atcgcccagg aagtcgccct cctcgccgcc
     2821 cgctccgatg tccgcgagga actcgaccgt ctctccgcac acatcgatgc cgcccacgcg
     2881 ctgctccgcg aaggcgccaa catcggccgc cgcttcgact tcctggtcca ggagttcaac
     2941 cgcgaggcca acaccctttg cagcaagtca gcctcggtcg cgctgaccgc caccggcctg
     3001 aagctgaagg ccacgatcga gcagttgcgc gagcaggtgc agaacatcga atgatgcgca
     3061 tcccaagacg aggcatctgc ctggtcctgt ccgccccctc cggcgcaggc aaaaccgcga
     3121 tcgccgacgc cctcctctcc accgaaaccg acctccaccg ctccgtcagc gtcaccaccc
     3181 gcgccccgcg ccccaatgag gtggacggca cccactatca cttcctcgac caggccggat
     3241 tcgaccgcat ggccgccgcc ggcgaactgc tcgaatgggc ccacgtcctg ggccggcact
     3301 gctatggcac gccccgcgct ccggtcgagc aagccctcag caacggccag gacgtagtgt
     3361 tcgacatcga ctggcagggc cacaaccagc tccgcgcagc ccttccccgc gacgtggtcg
     3421 gcgtgttcgt ccttcccccc tcactcgccg cccttgaggc tcgcctccat gcccgctccg
     3481 gtgatcccgc cgccgagatc gcccgccgca tgaagctcgc ccacgacgag atcagccact
     3541 gggccgagtt cgaccatgtc gttgtgaata ccgaccttgc ccacgcgatc gccgaggtcc
     3601 gcgcaatcct ccacgcggcc cgcctggcaa ccggacgcca gaccggcctc accgatttcg
     3661 ttcgcggact gatcgcgcca gccgatcgaa ctccgccacg ctgagcgtct cagcccggcg
     3721 atccgcggcg atctccgcct cctgcagcaa tgcctcccct ccgagctcac gcatcgcact
     3781 tcgcagcatc ttccgccgct gaccgaacgc ggccgccgtc acccgctcca tcaccttgaa
     3841 cagcgccgcc tccggctgcg ccgggcgcgg caccagcccc accacggcag agaatacctt
     3901 tggcggcggc acgaatgcgc tcggcggaat ccgtagcaac agcgccgcat cgcaggtcca
     3961 ctgcaccagt atggataatc gtccatacgc cgcggttccg ggggcagcgc agatccgctc
     4021 cgccacctcc tgctggaaca tcagcgtcat ccgctcgaaa gccgcggcct gccgcagcca
     4081 tcccaccagc aacggggtcg ccacattgta cggcaggttc gccacgatct gccgcggcga
     4141 cggcaccaat gcctcaacat ccatggccag cgcatcacct gcgactatgc gcatccgatc
     4201 gcccgtttcc gccgccagct cctgcatggc ggctacggcg cgcgtatcta tctccaccac
     4261 cgtcacgctc accgcctccg tgtccagcag cgcccgggtc aggccgccag gccctggccc
     4321 gacctccacc acatggcgcc ccgccagcgg cccggcctcg cggacaattc gccgcgtcag
     4381 gttcgcatcg agcagaaaat gctggccgaa tgaccgtttt gccgccagtc cgtgccgcgc
     4441 gatgacctca cgcagcttcg gctggtcact catgcaccgc cggagcgtat ctcgatcgtc
     4501 gcgtgtcgcc gcagatcggc ctggagctgc cgcgatagca gctcgatgcg ctcatcgagc
     4561 aactgcgccc gcacgtcctc cttcgattcc tgcgccatat tcttgtcgcc gcgcgagcac
     4621 acgatcagca ccgcgatacc gtcattggtg accagcggct gggtcgcctt gtcgagcggc
     4681 aacgatccaa gcacttgccg cagggcaggc ggattgatcg agtcgagacg cacctcgccc
     4741 gggtccgccg ggcgcgtcga accgtatgtc ttggccgcct gctccatggc gtcacagctg
     4801 tgaacggtgg cgctgatgga atgcgccttc tcgagcgtct gtttctgctg ctcggtcggt
     4861 gcctgcgggt tcagcgcggc ggtgaaaggc aggaacacct gccgcagcga gatggctgtc
     4921 accacgtcgt tgccgatctg ccgcttgccc tgcacggtca cgatggaaaa tccgccaggc
     4981 acgcggatcg gattgctgat cgcgcccacc ggcatctccg ccaccagctg cgcgacagca
     5041 ctgtcgagct gattaggctg cacccagccg agcgctcctc cctgcagcgc tgtctggttc
     5101 tgcgagaact gagcagcgac caccgggaac gccgcgcctg cgcgcagctc ggtaatcact
     5161 gtctcggcaa accgctgcgc atccgccgtc acggccggat cctccaccgg gatgaagatc
     5221 tcgcccaggc gatattccgg cttgcccacc tgctgggcca gcagccgctg ttgctccgcg
     5281 acttccgcat cgctgacctt caccttgtcg ccgagctcac cgcgcaacac ctcgccccag
     5341 ccaagctggg ttcggatctg atcgatcagc gtgcgctggc tcacgccatc ggcaagcagc
     5401 ttctgacgca gaccgcctgg cggcaggcca ttacgagtct cgatgccctt gatagcatca
     5461 gcgatcgact tgtccggaat cacgaccttc aggcgcagca cctcctgcat gcgaagccgc
     5521 tcgtcgatga gctggcgggt gatctgctgc ttgagccggc cgatgacgtc ctggttcagc
     5581 ggcagtcctg tcgagacggc gaacagccgg gcacgattgt ccacatcggc attgctgatc
     5641 acgtcgccat tgaccacggc tacaattcgc gtggtctcct ggcccgccgg gggcggcggc
     5701 tttgctggcg gcaccgctgc ggcgggtgtc acaagcagca acgccagggc ggcagctctc
     5761 caaattctat tcggcatgac ctgacctccc taatctcata acgcccggaa gccgaattgt
     5821 ccgattgtct tgaacgtcag ctggaccagc gccgtggtcg aaccgttgtc gccgttgaag
     5881 gaggtatagt ggcgataaag gtt
//
LOCUS       DATGEM010000666         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1131039, whole genome shotgun sequence.
ACCESSION   DATGEM010000666 DATGEM010000000
VERSION     DATGEM010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131039"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..911
                     /locus_tag="VKQ27_20575"
     CDS             24..911
                     /locus_tag="VKQ27_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY91389.1"
                     /translation="MAVQSDSLAWRPRISVVIPTLNEARNLPHVLESLPSDIHEIIIA
                     DGHSVDDTLEVVRQLRPEARIVMQTRSGKGNALACGCAAATGDIIALIDADGSTDAAE
                     IPDFVSALLAGADFAKGTRFAKGGGSEDITRLRSLGNRMLTGLVNLLCGTRYTDLCYG
                     FNVFWKKHLPVLGLDAETVLPEDDGGYLWGDGFEIETLINIRIAVAKLSVHEVPSFEH
                     SRIHGVSNLSAVSDGLRVLRTVLYERFIAPRRTSQLCASDVLPASDDSVAYAYAYAYA
                     EDGQTLPLRLPAAEIPRHI"
     gene            1236..>3125
                     /locus_tag="VKQ27_20580"
     CDS             1236..>3125
                     /locus_tag="VKQ27_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91390.1"
                     /translation="MLRNGHALIASASVTQFIGVVYWIFAARLYPTAVVGRNSVAISI
                     MLFLAGVAELNLMSTLVRFAPTSGDRTLRLIVSAYVTSISIGAVIGTIFVFLIPHVEP
                     QLDFLRSNPYIAVWFMLSVAMGTIFVLEDSALTGVRAATFVPIENAAFSILKLVLLIP
                     FAWFLQSAGVYLSWTVAIAVIIIPTNFYLFARAVPRHLSKYTVAIPAPLFRDIRAFLI
                     PDSLAALFMLASTVLLPLLIINRLGPSATAHYALAWIIGYALFLFSLNMGSSLVVETA
                     ADQSGLRQLSWRSMTHLTKLLIPMVAVIVAGAPYILLAFGRGYAEADVTPLRLLAVAA
                     LPAIVTNTAISATRSLRRMPMVLGIQVAICALVWGLSLVLIGRFGITGVAAAWLIAQT
                     ATALVLAVMPRSWMPSARSARPRAQALGRPATESPWSRVPRPVLLTVGGLLGISALVV
                     STVAVTGLVRSHSTHGQAIGSSSTPATHATGSGSAGPPSKSGTPLITQAISFTSSPPS
                     PAAVGGTYAVTAAGGKSGKPVTFSIDAATSSVCSVSGSIVTFNKPGSCVIDANQAGGA
                     GYQAAVQAQQKVTVSSLTAQVISFTSSPPSRARVGGTYAMTATGGGSGNPVAFSINPA
                     STSVCS"
BASE COUNT          522 a         1064 c          928 g          611 t
ORIGIN      
        1 tcagtcaatc atagggggaa ggcatggcag tccaaagcga ctcgctggcg tggcggccgc
       61 gaatctctgt cgtaattccg acgctgaacg aggcgcgtaa cctaccgcat gttctcgagt
      121 cgctgccttc cgatatccat gagatcataa tcgctgacgg tcactccgtc gatgatactc
      181 tcgaggtcgt tcgccaactg aggccggaag cccgcatcgt gatgcagacg cgaagcggca
      241 aaggcaatgc ccttgcatgc ggttgcgcgg cggcgaccgg agatattatc gcgctgatcg
      301 atgcggacgg ctcaaccgat gccgctgaga tcccggactt cgtatcggca ctgctcgccg
      361 gtgctgactt tgccaaggga acccggttcg ccaagggcgg cggcagcgag gatattaccc
      421 ggctgcgcag ccttggcaac cgtatgctga ccggcctcgt gaatctgctt tgcgggacca
      481 ggtatacaga tttgtgctat ggcttcaatg ttttctggaa gaagcatctt cccgtcctcg
      541 ggctcgatgc agagactgtc ctgccggagg atgacggagg ttatctctgg ggcgatggct
      601 tcgagatcga gacgctgatc aacatccgca tcgccgtggc gaaactgtcg gtccatgagg
      661 ttcctagctt cgaacattcc agaattcatg gcgttagcaa cctgagcgca gtcagcgacg
      721 ggctacgggt cctccggacg gtcctctatg agcgattcat cgcgccgcgc cgcacgtccc
      781 aactgtgcgc cagcgatgtc ttgcccgcca gcgatgactc ggtcgcctac gcctacgcct
      841 acgcctacgc cgaggacggg caaacgctgc cgctgcgtct tccggcggct gaaattcccc
      901 ggcatatctg accgggcgcc gtggagagta cgcaggcaga gtcgactgga cgagatgacg
      961 ggcggccagc gccagatcgg cgggtcggcc gccgcgagtg tgccgtcgcg accgacgagt
     1021 cccaggcacg accgggcgcc gcgaccgagg acaccatcca gttcaacacc atccagttca
     1081 tagcacccgc cgggtactac acggcgcccc gcgttccccg gaccccaggc accaccggac
     1141 ccaggcccgg cggtgagccg ccggaaagcg actacagtcc ggagtcaatc gtacgcaaac
     1201 cgctgcgggt catgcgtgac gtcctgaagg accccatgct gcgcaacggg catgcgctca
     1261 tcgccagcgc ttccgtgaca caattcatcg gtgtcgtcta ctggatattc gccgcacgtc
     1321 tttacccgac cgccgtggtc ggacgaaatt ccgtggctat ttccataatg ctcttcctcg
     1381 ccggcgtagc ggagctgaac ctgatgagca cgctcgtcag gtttgcgccc actagcggag
     1441 accggacact tcgccttatc gtcagcgcgt acgtcacgag tatttcgatc ggcgccgtta
     1501 tcggcaccat cttcgtcttc ctgatcccgc acgtggagcc gcagctcgat ttcctgcgct
     1561 ccaaccctta cattgcggta tggtttatgc tctcggtcgc catgggtacc atattcgtcc
     1621 tggaggacag tgccctcacc ggggtccgtg ccgcaacctt cgtccctata gaaaatgctg
     1681 ccttctcaat ccttaagctc gtcctgttga tcccgttcgc ctggttcctc cagtcggcgg
     1741 gtgtctacct atcgtggacg gtcgcgattg ccgtaataat aatcccgacc aatttctacc
     1801 tcttcgcccg agcggtgccg cgacatctga gcaaatacac ggtcgccatc ccggcgcccc
     1861 tgttccgcga catccgcgcg ttcctgatac ctgactccct ggccgcgcta ttcatgctgg
     1921 cctcgaccgt attgctcccc ctcctgatca tcaatcgcct cgggccgagt gctaccgcgc
     1981 attacgcgct ggcgtggatc atcggctatg ccttgttcct cttcagcctc aatatggggt
     2041 cttccctcgt cgtcgagacg gccgccgacc agtccggcct gcgtcagctc tcctggcggt
     2101 ccatgacgca cctgacgaag ctcctcatcc cgatggtggc cgtgatcgtg gccggcgccc
     2161 cgtacatcct gctcgcgttc ggccgcggat acgccgaggc cgacgtgact ccgctgcggc
     2221 tgctggcggt ggcggcacta ccagcgatcg tcactaatac ggcgatcagc gcgacccgtt
     2281 ccctgcgcag gatgcccatg gtcctcggca tccaggtggc tatctgtgct ctggtctggg
     2341 gcttgagcct ggtgctcatc ggacgcttcg gaattaccgg ggtggccgcc gcatggctca
     2401 tcgcccagac ggcgaccgcc ctggtgctgg cggtcatgcc ccggtcttgg atgccgtcgg
     2461 cgcggtcggc tcgcccccgc gcacaggcgc tgggccgtcc tgcaacggaa tcaccatggt
     2521 ctcgtgtgcc acgccctgtc ctgctcaccg tgggtggctt gctcggcata tcggcactgg
     2581 tcgtgagtac agtcgcggtc accggcctgg tccggagcca ctcaacccac ggccaggcca
     2641 tcggatcgtc aagcacaccc gcaacacatg ccactggctc tggctctgcg ggcccgccat
     2701 ccaagtctgg caccccgctg atcacgcagg cgatctcgtt cacttcctcg ccgccatcac
     2761 cggcggcagt aggcgggact tacgccgtga cggccgccgg cggcaagtca ggcaagccgg
     2821 tcacgttcag catcgacgcc gccaccagct cggtgtgctc ggtctccggg tctatcgtca
     2881 ccttcaacaa gccaggcagc tgcgtcatcg acgctaacca ggccggcggc gccgggtacc
     2941 aggcggccgt ccaggcccag cagaaggtga cggtcagctc gctgaccgcg caggtgatct
     3001 cgttcacctc ctcgccgcca tcgcgggcga gagtaggcgg gacctacgcc atgacggcca
     3061 cgggcggcgg gtcgggcaac ccggtcgcct tcagcatcaa cccggccagc acctcggtgt
     3121 gctcg
//
LOCUS       DATGEM010000667         6291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1534956, whole genome shotgun sequence.
ACCESSION   DATGEM010000667 DATGEM010000000
VERSION     DATGEM010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534956"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VKQ27_20585"
     CDS             <1..1436
                     /locus_tag="VKQ27_20585"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HLY91391.1"
                     /translation="VDLSCLKGFCPSFVTVAGPPSAPDADPHWQAREGELCAGLPQPR
                     MPSTTKPWRALFAGIGGGGIVTSGAILAMAAHLEGKAVRTLDFTGLAQKNGTVVAHVQ
                     IADDEAALDVVRVPLGTADVMIAADLAVAAGPGVLERNAATAAVIGNLDLAATAAFKH
                     DANLSIDAVLHRRTIERVTDAGASSWLHAVRLAEHLFGNAQAMNTMLLGLAWQRGLVP
                     VGEAAIMRAIELNGAAVSLNRRAFVWGRILAEKPALEDEILVHVREKPPATLDALIEA
                     RAAALAAYQSRRLANRYHRLMQETMARETEALGSADRLSRAAAEGLYRVMAYKDEYEV
                     ARLHASATYGEKPVFHLSPPLITGFDPATGRRRKIALPGWLALPLFRVLQHGKHLRGT
                     PLDVFGRQEERKLERALVDQYIGDLHTVLGSLRPETLDVAVAIAQLPDMIRGFGPVKE
                     ANRVKAEQQRQTLLARLAPVPMVMAAD"
     gene            complement(1480..2346)
                     /gene="motA"
                     /locus_tag="VKQ27_20590"
     CDS             complement(1480..2346)
                     /gene="motA"
                     /locus_tag="VKQ27_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HLY91392.1"
                     /translation="MLAIGAFVLLLGCVFGSYTVSGGSFDVLVEAVPFEMWTIGGAAL
                     ASFVMANSMHDLKHAVVGFKKILGGAAFHKTDYIDLLSLLYFLVRLASTKGNMALEPH
                     IEKPEDSAAFQKFPKIMRNHFAAAMICDYLRMVGMNADDPNQIEDVMARELKKTLNEE
                     LHGAHALQMMADGLPALGIVAAVLGVIKTMSHIDRPPAILGGMIASALCGTFLGVLLA
                     YGLAGPCASRLKGVIEEESKYYEVIRAVLVTHLHGNAPQVSVESGRKMVPTEHMPSFQ
                     ELEEAVQSVQIT"
     gene            complement(2356..3414)
                     /locus_tag="VKQ27_20595"
     CDS             complement(2356..3414)
                     /locus_tag="VKQ27_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HLY91393.1"
                     /translation="MARRGDKKSKGQVLIRKEEIIEAQGHGGAWKVAYADFVTAMMAF
                     FLLMWLLNATTEEQRKGLADYFSPSAVLSHNSSGTGQPFGGKTAFSEGAMVSDRGAVQ
                     VTLGQRPVIDPADQDDSEVIAQPRPHRDDVESKAPDQGGRLAAQPQPAPSREIAVVRP
                     PKPGEAAAGNVGGRKPTDAEVRAELEKREKQAFDQAAQQIREAVRADPQLAELAGQLQ
                     IDITPDGLRIQILDAEHEPMFPLGSTVPNDHARLLLQKVAPVLAKMTGDVSISGHTDA
                     AQFAGSGRSNWELSADRANATRRLLEEAGLQQTRIRSVTGNAERDLLVPADPMAAANR
                     RISIQVLRSVPLSSIAPP"
     gene            complement(3439..4887)
                     /locus_tag="VKQ27_20600"
     CDS             complement(3439..4887)
                     /locus_tag="VKQ27_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY91394.1"
                     /translation="MTGASPPCAWPLPSIIAALIALTLLRLAIAAAVPLAPDEAYYWV
                     WSHALAPGYPDHPPMIALWIRLGTSIAGDGPLGIRLLGPISVALASVLLANATDALFP
                     GNRSGVRAAVLLNATLLFGVGAIIMTPDAPLLVFWTACLWAIARLQQSGDARWWLAIG
                     LFAGLALASKYTAALLWLGIVLWVLITPSLRHWLRHPMPWLGTIVAVAIFAPVVFWNA
                     GHDWTSFGRQGGRLGDWHPGNAVRFMTELIAGQFGLLTPLAFAFCVGGIVLAARNAWR
                     SRDPAWTLLAVLTIPGAILFLQHALGDRVQGNWPAIIYPAAAIAATQLRTDVWRRLWS
                     PAIALGFAITLLAYVQALFAVLPLPLRLDPIALILSGWDRLAAQVDAIRQETGAQYIA
                     ADQYGVAAELAYALKAPVVGLEPRWAMFDLPRAPIAGQVGVLVQTGGDLDRTLWSSVT
                     ELAVAKRGIQTFGIYRVTTSASPVDARLLVRN"
     gene            complement(4884..6011)
                     /locus_tag="VKQ27_20605"
     CDS             complement(4884..6011)
                     /locus_tag="VKQ27_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLY91395.1"
                     /translation="MSDLISSPGMPPCGAPVLTVVVPSYNERPNVAPLVARLDTALRG
                     ISWEVVFVDDNSPDGTVGEVRRMAQTDSRIRCIRRIGRRGLASAVIEGALSSSAHYVA
                     VMDGDLQHDETRLPEMLTGLQSGQYDLAVGSRHVAGGDNAGLSGRWRHMLSDGGIRLA
                     QMFLPIRLSDPMSGFFMLPRPLFEQLAANLTGQGFKILLDLTLSAPAPLRVIEVPFVF
                     HERVAGESKLDALVLTQFAGLLLDKVFGGLLPLRFISFGLVGALGVLVNLAVLGAALH
                     ISGVGFETAQAFGTVVAMVFNFLLNNEITYRDQRLRGPRLWRGLLIFMLVCGVGAIAN
                     IGIATTLYDTHTNWTIAGAVGAVIGVVWNYAVSATLVWRSR"
     gene            complement(6027..>6291)
                     /locus_tag="VKQ27_20610"
     CDS             complement(6027..>6291)
                     /locus_tag="VKQ27_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HLY91396.1"
                     /translation="ITVFAIVGLDSQKEAVLSAFEQLVAGWPEVRECHMIRGGGDFLL
                     KFVARDTAHENQLTQRLTGSPTVTRVQTLQTIRTSRSLAGVPL"
BASE COUNT         1165 a         2049 c         2006 g         1071 t
ORIGIN      
        1 atgtcgatct ctcctgcctg aagggcttct gcccgagctt cgtcactgtt gccggcccgc
       61 cgagcgctcc cgacgccgat ccgcattggc aggcgcgcga gggcgagttg tgcgccggac
      121 tgccgcagcc acgcatgccg tcgacgacga agccttggcg cgcattgttc gccggcatcg
      181 gtggcggtgg catcgtcacg tctggcgcga tcttggcaat ggcagcgcat ctggaaggca
      241 aggcggttcg tacgctcgac ttcaccggcc tggcgcagaa gaacggcacc gtcgtcgcgc
      301 acgtccagat cgccgatgac gaggctgcac tcgacgtggt gcgtgtgccg ctcggcactg
      361 ccgatgtgat gatcgcggcc gatctcgcgg ttgccgctgg gcctggcgtg ctcgaacgca
      421 acgctgcaac cgccgctgtg atcggcaatc tcgatctcgc ggccactgcc gcgttcaagc
      481 acgacgcaaa tctctcgatc gacgccgtgc tgcaccgtcg cactatcgaa cgcgtaactg
      541 atgcgggcgc atcgtcctgg cttcacgccg tgcgtctggc cgagcatctg ttcggcaacg
      601 cgcaggcgat gaacacgatg ctgcttggcc tcgcctggca gcgtggcctg gtgccggtgg
      661 gcgaggcggc gatcatgcgc gcgatcgagc tgaatggcgc cgccgtgtca ttgaaccggc
      721 gcgcgttcgt ctggggccgc atcctcgccg agaagccggc tctggaagac gaaatcctgg
      781 tgcatgttcg cgaaaagccg ccggcgaccc tggacgcgct gatcgaggcg cgcgccgcag
      841 cactggccgc ataccagagt cggaggctgg ccaaccgcta tcaccgactg atgcaggaaa
      901 cgatggcccg cgaaactgag gcattaggta gcgcagatcg cctgtcgcgc gccgcggccg
      961 agggcctgta tcgcgttatg gcctataagg atgaatacga ggtcgcgcgc ctgcacgctt
     1021 cggcgacgta tggcgaaaag ccggtgttcc atttgtcacc gccgctgatc acgggcttcg
     1081 atcccgccac cggccggcgc cgcaagatcg ccctgccagg ttggctggcg ctcccgctgt
     1141 tccgtgtcct gcagcacggc aagcatctgc gcggcacgcc gctcgatgtg ttcggccgtc
     1201 aggaagagcg caaattggag cgcgccctgg tcgatcagta tattggcgac ctgcacacag
     1261 ttctcggatc gctccgccct gagacgctgg acgtcgcggt tgcgatcgcc cagttgcccg
     1321 acatgatccg tggcttcggt ccggtgaagg aagcgaacag ggtgaaagca gaacaacagc
     1381 gccagacact gctggcacgc ttggcgccgg tgccgatggt gatggccgct gattagctgt
     1441 cacaccgtct cggcgtttgt tcagctgcgc gttccgtact caggtgatct gcactgactg
     1501 aaccgcctcc tccagctcct ggaatgacgg catgtgttcc gtcggcacca tcttgcggcc
     1561 tgactcgaca ctgacttgcg gcgcattgcc gtgcagatgc gtcacgagca cggcgcggat
     1621 cacttcatag tatttcgatt cttcctcgat cacccctttc agccgcgatg cgcatggccc
     1681 agccagccca tacgccaaca ggacgcccag gaacgtgccg cacagcgcac tggcgatcat
     1741 gccgccgaga atggcaggcg gcctgtcgat atgcgacatc gtcttgatca cgcccagcac
     1801 cgccgcgacg atgccgagcg ccggtaaacc atctgccatc atctgcaatg catgcgcgcc
     1861 atgcagttcc tcgttcagtg tcttcttcag ctcgcgcgcc atcacgtcct cgatctggtt
     1921 gggatcatcg gcgttcatcc cgaccatgcg caggtaatcg cagatcatcg cggcagcgaa
     1981 gtggttccgc atgatcttgg ggaacttctg gaacgccgcg ctgtcttccg gtttctcgat
     2041 atgcggctcg agcgccatgt tgcccttggt gctggcgagg cgaacgagaa aatacagcaa
     2101 gctcagcaga tcgatgtagt cggtcttgtg aaacgcagca ccgccgagga tcttcttgaa
     2161 gccgaccacc gcatgtttca ggtcgtgcat gctgttggcc atgacgaacg acgccaacgc
     2221 ggcgccgccg atggtccaca tctcgaacgg cacggcttca acaagcacat cgaagctgcc
     2281 gcccgacacc gtgtaactgc cgaacacgca gccaagcaga agcacaaaag cgccgatagc
     2341 cagcatggca acttcttatg gcggcgcgat cgaggaaagc ggcaccgacc gcaggacctg
     2401 aattgagatg cgacggttcg cggccgccat cggatcggca ggaaccagca ggtcgcgttc
     2461 ggcatttccg gttacgctgc ggatacgggt ttgctgtaag ccggcctctt ccagcagccg
     2521 ccgcgtggca ttcgcacgat ccgccgacag ctcccagttg ctgcggccag agccggcgaa
     2581 ttgcgctgca tcggtatgcc ctgagatcga gacgtccccg gtcatcttcg ccagcaccgg
     2641 tgccaccttc tgcaacagca accgggcatg gtcgttcggc accgtcgatc ctaaaggaaa
     2701 cattggctca tgctcagcgt cgagtatctg gatgcgcagt ccgtcaggtg tgatgtcgat
     2761 ctgcaactgc ccagccagtt ctgccaattg cggatctgcc cgcaccgcct cgcggatctg
     2821 ttgcgccgcc tgatcgaatg cctgcttctc gcgtttctcc agttcggcgc gaacctcagc
     2881 gtcggtgggt ttcctgcccc cgacgttgcc ggcggcggcc tcaccgggct tcggcggcct
     2941 gaccaccgca atctcgcgtg agggcgcggg ttgcggttgc gccgccaagc ggccgccctg
     3001 atcgggcgcc ttgctctcaa catcgtcgcg atgcgggcgc ggctgggcaa tcacttctga
     3061 atcgtcctga tcagccggat cgatcacggg gcgctgtccg agcgtcacct gaacggcgcc
     3121 gcgatcggac accatcgcgc cctcgctgaa cgccgtcttg ccgccgaatg gctggccagt
     3181 gcccgacgaa ttatgcgaca gcacggccga cggactgaaa tagtcggcaa gccctttgcg
     3241 ctgctcttcc gtcgtggcgt tgagcagcca catcagcagg aagaacgcca tcatcgccgt
     3301 tacgaagtcg gcataggcga ccttccaggc accgccgtgc ccctgggcct caataatttc
     3361 ttccttacgg atgaggactt ggcccttgct cttcttgtcg cccctacgtg ccatggacga
     3421 catcgtgcac gttgcagttt aattcctcac taagaggcgg gcatcgaccg gactcgccga
     3481 ggttgtgacg cggtagatgc cgaacgtctg gatgccgcgc ttcgccacgg caagttcagt
     3541 gaccgatgac cacagtgtgc gatcaaggtc accgccggtc tgcaccagca cgccaacctg
     3601 cccggcgatc ggcgcacggg gcaggtcgaa cattgcccaa cgcggctcga gtccgacaac
     3661 tggcgccttc agcgcatagg ccagttcggc ggcaacgccg tactgatcgg ctgcgatgta
     3721 ctgcgcaccc gtttcctggc gtatggcatc aacctgcgcc gccagcctgt cccatccgga
     3781 caggatcagt gcgatcggat cgaggcgtag cggcaaaggc agaacagcaa acaaggcctg
     3841 cacatatgcc agcagtgtga tggcgaagcc gagcgcgatg gcgggcgacc atagacgtct
     3901 ccatacatcc gtccgcagtt gcgtagcggc gatggcggcg gccggataga tgatggccgg
     3961 ccagttgcct tggacgcggt cgccgagcgc gtgttgcagg aacaggatcg cgccggggat
     4021 tgtcagaaca gccagcaggg tccaggcggg atcgcggctc cgccaggcat tgcgcgcggc
     4081 aagcacgatg ccgccaacgc agaacgcgaa tgccagcggc gtgagcaggc cgaactggcc
     4141 ggcaatcagc tcggtcatga agcgcacagc gttgccagga tgccaatcac ccaatcggcc
     4201 tccttgccgg ccaaagctgg tccagtcgtg accggcgttc cagaaaacca ctggcgcgaa
     4261 gatcgcgacc gcaacgatgg tgcccagcca tggcatggga tggcgcaacc agtggcgcaa
     4321 cgacggcgtg atcagtaccc agagaacgat gcccagccac aacaaggccg cggtgtactt
     4381 gcttgccaag gcgaggccgg cgaacaggcc gatcgccagc caccagcggg catcaccgct
     4441 ctgctgcaat cgagcaatgg cccagaggca ggccgtccag aacaccagca gcggcgcatc
     4501 cggcgtcatg atgatcgcgc cgacgccgaa cagcagtgtt gcgttcagca gcaccgctgc
     4561 gcgcacgccg ctgcgattgc cggggaacag cgcatccgtg gcattggcga gcaggacgga
     4621 cgcgagcgcg acggaaatcg ggccgagcag gcggataccg agcggtccat caccggcgat
     4681 cgatgtgccg agacggatcc acagcgcgat cattggcggg tggtccgggt aaccgggcgc
     4741 caaagcgtgt gaccacaccc aatagtaagc ctcgtccggc gcaagcggca ccgccgcggc
     4801 aatggcgagc cggagcaggg tcagcgcgat gagggcggca atgatcgagg ggaggggcca
     4861 ggcgcatggc ggggaggccc ccgtcatctg gaacgccaga ccagcgtggc cgagaccgcg
     4921 tagttccaca ccacgccgat gaccgcgcca acagctccgg cgatcgtcca gttggtgtgt
     4981 gtgtcataca gcgtcgtcgc gatgccgata ttggcgatcg cgcccactcc gcacaccagc
     5041 atgaatatca gcagaccccg ccacaggcgt ggaccgcgca ggcgctgatc gcggtaggtg
     5101 atctcattgt tcagtaagaa gttgaacacc atggcgacga cggtgccgaa cgcctgcgcc
     5161 gtttcgaaac cgacgccaga gatatgcagc gcggcgccga gcacggccag gttgacgagg
     5221 actccgagcg cacccaccag cccgaaggag atgaagcgca gcggcaacag tccaccgaac
     5281 accttgtcca ggagcaatcc ggcgaactgc gtcagtacca gcgcatccag cttgctctcg
     5341 ccggcgaccc tttcgtggaa cacgaagggc acttcgatga cgcggagcgg cgctggtgcg
     5401 ctgagcgtca gatccagcag gatcttgaag ccctggcccg tgaggttggc agccagctgc
     5461 tcgaacagcg gccgcggcag catgaagaag ccgctcatcg gatcgctcag ccggattggc
     5521 aggaacatct gggccagccg gatgccgcca tcggacagca tatggcgcca gcggccggac
     5581 aggccggcat tgtcgccgcc ggcgacatgg cggctgccga cggcgaggtc gtactggccg
     5641 ctttgcaggc cggtcagcat ttccggcaac cgggtctcgt cgtgttgcag gtcgccgtcc
     5701 atcaccgcca cgtagtgtgc cgaggatgac agggcgccct cgatcactgc cgatgccaga
     5761 ccgcgacggc cgatcctgcg gatgcagcgg atgcgcgagt cggtctgggc catgcgccgg
     5821 acctcgccca ccgtgccgtc ggggctgttg tcgtcgacga acacgacctc ccacgagatg
     5881 ccacgtaacg ccgtgtccaa ccgggcgacc agaggcgcca cattggggcg ctcgttgtag
     5941 ctgggcacca cgaccgtgag gacaggcgcg ccgcacggtg gcatgccggg gctgctgatc
     6001 aggtctgaca tggagcggct gatttcctaa agcggcacgc ctgcgaggct gcggctggtg
     6061 cggatggtct gcaaggtctg cacgcgcgtc accgtgggcg atccggtcaa cctctgcgtg
     6121 agctggttct catgcgcagt atcgcgtgcc acgaacttca gcaggaaatc gcctccgcca
     6181 cggatcatgt ggcactcacg cacctccggc cagcccgcga cgagctgctc gaaggcggac
     6241 agcacggctt ccttctggct gtccagtccg acaatggcga acacggtgat c
//
LOCUS       DATGEM010000668        21595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_967814, whole genome shotgun sequence.
ACCESSION   DATGEM010000668 DATGEM010000000
VERSION     DATGEM010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 21595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21595
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967814"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VKQ27_20615"
     CDS             <1..694
                     /locus_tag="VKQ27_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91397.1"
                     /translation="KRIPGLNTDSLHDCFTDASVWANFEPGCLTLCSPDPQAIRMPDD
                     KINVLTVTLPTNFKAARFDSDASTNIMRQFQREIEAIRFNAGDAQIDLPVKLKVHESI
                     FVPLAKWAMLCTGNYRCVKKDAAVSIKEAVHTDLATSERVYNWVRELCILLGASPDDL
                     VPFEKYAAAANGLIRPSSAARALFAGAPNIERVDRLVQSIGAQRGMRNAELDATVALV
                     DARLEMNRKAAA"
     gene            786..1208
                     /locus_tag="VKQ27_20620"
     CDS             786..1208
                     /locus_tag="VKQ27_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91398.1"
                     /translation="MERVVVKNFEMWGRLVKTWATGDDYVRDGNSYPRPADLAEFKRQ
                     VQQANCEMTMPDRLTKLDMVQGDDVTLVVRLPPAQMIVDSEHVLAELGSVSVGSPYPL
                     PTFYRDAWGVQQQINLSASQLLLFHNERIGEYTINNCM"
     gene            complement(1212..3263)
                     /locus_tag="VKQ27_20625"
     CDS             complement(1212..3263)
                     /locus_tag="VKQ27_20625"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HLY91399.1"
                     /translation="MADAALRMDLTEPTPRWTGPRLCISMFGPMALSFSGRPVRLIGR
                     KARAVLGCLVLNPTSRETRERLVGLLWSDSDEDRARTTLRQTLREIRQAFSEAGYGGL
                     QANRLTIELDPASVEVDLASVIEEAEAHRAHPLLLGQARLADTLLLDLDDIDAEFRVY
                     LLAKRHSLQQRLIRALETGMRRDDLDRPTRSRLAEATINLDPTHEEACRLLMQWRAEA
                     GDTGGALKLYKQLWDLLDDEYGMEPSAPTQQLVADVKSGAFEPAAVPIAANLSAAPAV
                     RSGSKLVLSVGVVDVRGLAADKPHLIEGFRQHLIACLVRFREWYVTDQPPQLIAANEP
                     SDVSASYEINILAQETGDSIGLVLILKEVDSNIYVWSETFELKLENWFELQRRIVTRI
                     ATALKVHLSTERIMRFSAEPDVALGIYDRWLRCQSQILTFSPEDWHRAEHEFNQIIAA
                     APNFTGAYCSLAQMNNAIHIVHPGVLRSPDRTLRSLELARKAVQLDPIDTRAQLCLGW
                     SHALAGQFGSADLHMDMAGELNPHDPWTLIAVALFEAFRGRFESATGLARQALDRSLR
                     PSRTHWAYQVSIAYLSGDNEGALQAADMAMDAIKTLPAWRAAALYRLGRQQEAQESAR
                     RFLANIRGAWRGDRQPTDETIVCWLLHLYPISERAVWEALRDALRGASLPAGTAQYGN
                     W"
     gene            complement(3311..4573)
                     /locus_tag="VKQ27_20630"
     CDS             complement(3311..4573)
                     /locus_tag="VKQ27_20630"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HLY91400.1"
                     /translation="MPPPDAAELLSLLGYLDGDAMAARHRVSLLRAAARLRRLFRIEA
                     PDAPGLCFFGGEAETPGAGSAIASFAGSGVDWRRAFESCVGEAIEYLSQIEASDTNRD
                     VGSFVERAVLLDAPAERFIGALLDHCGVSRSRPIAWTHAARLHGGGEAPMPLDLCVRR
                     IAEAQDFSPPLRLSVGCAAGVSATGATLHALLELVERDATAQWWRGGVRGHLVPLEWA
                     ASRDAALQIERLRGTASSRRTWLLDITTDLGVPVVAAVSARHDGYGFAAGFACRPTMA
                     AAATAALFELCQMELGLHLAQSRLRENGESALGTGDRAHLRRAQAIDTSACRLLHPGP
                     PTGLAHDDEGADGDEALLSRLVARLVAQGIESYAIDLTRPEFGVPVVRVIAPALQLDP
                     CAIVSARLRDAMMRCRELGAETASVALF"
     gene            4806..5705
                     /locus_tag="VKQ27_20635"
     CDS             4806..5705
                     /locus_tag="VKQ27_20635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLY91401.1"
                     /translation="MPFPGSSLPLFDVGTLFALVGPPAGSSSPARPHDRPIAVLGGCL
                     ALGCYIAMIAHLGLAPHGVAPIIAVFIAALASSVVGFAFSAAGGAILFHVLPSPVEII
                     QILLVCSIANQALSVWALRRAISWRATAPFLLSGMLGAPAGAWLLLHSEPHAYLLAFG
                     VLLIGYSLSALIRPQTQLRRRWLGGDWLVGLLGGLTGGFAASPGAPLSIWLTLLGWDK
                     LRRRAVYQPFILVIQCVALVSMQAMARQVQPQGSITGVSLYLPASLLGTWYGLSIFAA
                     LNDNQFTLAINLLLIVSGLALVL"
     gene            5846..6001
                     /locus_tag="VKQ27_20640"
     CDS             5846..6001
                     /locus_tag="VKQ27_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91402.1"
                     /translation="MLLMQVSPLVARRFAVQMHERACHGTDQTWADFWLHVIHQLVTP
                     GNMEGAK"
     gene            5998..6384
                     /locus_tag="VKQ27_20645"
     CDS             5998..6384
                     /locus_tag="VKQ27_20645"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HLY91403.1"
                     /translation="MTAPILKSWLMLFGCLAAGETLHRIAGIHVSGAVIGMAILVAAL
                     TVTGGPSEPFEQAASSLLKHLPLLFVPAGVGVIQQVTIIRSQILPIAIALPVSTAFAM
                     AIAAVVVERMTAREPLKERVLQMEEQ"
     gene            6381..7103
                     /locus_tag="VKQ27_20650"
     CDS             6381..7103
                     /locus_tag="VKQ27_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HLY91404.1"
                     /translation="MITPIARQWMTDAMQPATWMTLTVLAYVLAIELQRLCKATPLLN
                     PTLLSIAAVIAVLLLTRSNYGAYFRAVEPIHLLLGPATVALGLPLYKQLHVIRANAMP
                     VMAGVIVGAVIACGSAVAIAWLLGGSPDLLLSLAPKSVTAPIAMSISQEIGGSPTLTA
                     SLVITTGIIGAVLCRWLFDWVGVKSWPARGLATGIAAHGIGTGAILATNEVAGAFAGL
                     AIGLTGLFTAIVLPVVASWFPG"
     gene            complement(7111..8676)
                     /gene="murJ"
                     /locus_tag="VKQ27_20655"
     CDS             complement(7111..8676)
                     /gene="murJ"
                     /locus_tag="VKQ27_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLY91405.1"
                     /translation="MLRGVLTVGGWTMGSRILGFARDMLIAAILGTGPIADAFLVALK
                     LPNLFRRLFGEGAFNAAFVPEFAGMLAAEGRPSAHRFAEETFAVLAFWLGVLTIIGEI
                     FMPQMMTVLAPGFSADPAKFTLTVALSRITFPYLLLICLAALVSGALNGLDRFTAASA
                     SYVLFNIISIACMLWLTPYVPTAGHALAWGVTASGVAQLGLLMWALRNAGMALRLPRP
                     RLTPQIRVLLRRMLPGLIGAGVTQLNLAVDVIIASLLPAGTVSLLYYADRVQQLPLGV
                     IGTAVGTALLPLLSRQVRAGERDAALGTLNRAIEYALFLTLPAAVALIVTAYPVMWVL
                     FARGAFNSESARLSAQSLAAYAIGLPAFVLVKVLAPGFFARGDTSTPVKIGIAAVALN
                     LAMNVAFMVPLQHIGPALATSLAAMFNTGWFAWVLVRRGHLRLDAQLRRRGLRMIAAA
                     GAMAVVLIIIQRMLFATPPHGVARFEALAVLVAGGLLAYVAAAHLFGAWDLREFQRMM
                     GPRWRALRQRNRA"
     gene            complement(8676..9638)
                     /locus_tag="VKQ27_20660"
     CDS             complement(8676..9638)
                     /locus_tag="VKQ27_20660"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLY91406.1"
                     /translation="MSSVAVIGAGAWGTALAIQAERAGNDVSLWARDQQRAGMISVTR
                     ENARLPGVRIPEPILVTGAAPAAADITLLAVPMQHIRTVLPQLPPGLVPLVACAKGLE
                     TGTLRLPLEIIEELRPGTPAAVLTGPNFAHEIAAGLPAASVVAATDPILREHVATTLS
                     TPSFRLYGNDDPTGAQVGGAAKNVIAIAAGTVIGAGLGENARAALITRGLAELARLAA
                     ALGGRRETVMGLSGLGDLLLTCTGPASRNFSLGLALGRGEVLADVLAARSAVTEGVAT
                     APALVARAAGVDLPICTAVAALLSGATTLKEAMAALLSRPRRDE"
     gene            complement(9631..10545)
                     /gene="rlmJ"
                     /locus_tag="VKQ27_20665"
     CDS             complement(9631..10545)
                     /gene="rlmJ"
                     /locus_tag="VKQ27_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HLY91407.1"
                     /translation="MNYRHAFHAGNFADCVKHAVLVWLLRALQRKPAPIFVLDTHAGV
                     GRYDLTTGPAARTGEWRNGIARLLDDPPAVLRDYLDAVRAPPSAVAASVDQHSQMAGS
                     MAGHDEGWPDDRWEAAHYPGSPCLIRALLRPDDRLACCELHSEDASALRQLFARDRHV
                     AVHQRDAWEALGALLPPKERRGLVLIDPPYEDRDEFAQVGRGLAASHARFANGVFVAW
                     YPIKQRAPVRQFLSGMQQSGIRDIVSAELCLREPVDAARLNGCGLLVTNPPYRFEEEV
                     RPILEALLARLGDREPGEYATITRITNE"
     gene            complement(10594..11346)
                     /locus_tag="VKQ27_20670"
     CDS             complement(10594..11346)
                     /locus_tag="VKQ27_20670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLY91408.1"
                     /translation="MELRGAVALVTGGNGGLGQRICHALAKEGVHVAVMYAQSREQAE
                     GVARELATSYQINAAAFPCDITDSGAIAKLLQDVAQRFGRIDILVNDAAYNKAIPFTD
                     LDSLTEDVWDKIMAVNLTGPMRLTKAIAPIMKRQGRGRIVNISSVAGLGPQGSSIAYA
                     VSKAGLIHLTRCMAVALAPETLVNCVAPGLLEGTRATANLRPEMIERSASSSLLKKAA
                     DKDDCADMVVTMCRTETMTGQTIVIDSGRTFH"
     gene            complement(11414..12037)
                     /locus_tag="VKQ27_20675"
     CDS             complement(11414..12037)
                     /locus_tag="VKQ27_20675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HLY91409.1"
                     /translation="MDGVRGPPSWRIHQLTCDRAATWSISASGNRRVTFALEEGEISD
                     RNLEGGLSLMDDAPIKLGMRLSSPGEFIREEILAELGLPVAWAAALLGVRRATLSDLV
                     NGKAGLSPEMALRASRRRSASTWIRCCACRHGTTATLCVSAKGRSWLSVTCRLAERRL
                     RRGDCDHRVHGLVLTECSESDLGKIGTTWIRIVSSAVLLAMTVRLVP"
     gene            complement(12197..13603)
                     /gene="fumC"
                     /locus_tag="VKQ27_20680"
     CDS             complement(12197..13603)
                     /gene="fumC"
                     /locus_tag="VKQ27_20680"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLY91410.1"
                     /translation="MTEFRRETDSMGAVDVPADKLWGAQTQRSLEHFSIGHDLIPREM
                     ITAYATLKRSAAIANHNGGRLHDKEFGLIIKTCDEILAGQHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCCQLAGTALGSKAPVHPNDHVNMSQSSNDSFPSAMYIAAAVNVTSRL
                     VPAVKGLHDAIAAKAKEWDSIVKIGRTHMQDATPLTLGQEWSGYAGMLSDDVERIDDA
                     LKGVYKLALGGTAVGTGINAAPGFAEAATAEIALYTKLPFISAPNKFTVQGAHDALVQ
                     LSGTLRTLATSLYKIGNDIRLMSCGPRAGFAELLIPENEPGSSIMPGKVNPTQAEALT
                     MLAVQVMANDVAVGFGGAGGYLEMNVYKPLIISNITQSITLMTDGCTNFRKFLVEGTK
                     PNLKKIEEYVERSLMLVTALAPVIGYDKASQIAHYAMDHDLTLKEAALKLGFVTEQEF
                     NRVVDPSKMVRPYVATAS"
     gene            complement(13619..15112)
                     /gene="mqo"
                     /locus_tag="VKQ27_20685"
     CDS             complement(13619..15112)
                     /gene="mqo"
                     /locus_tag="VKQ27_20685"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HLY91411.1"
                     /translation="MSESIDTPDVILIGAGIMSATLGVLLKELQPSLTIAMFEAMPDC
                     AQESSEAWNNAGTGHAANCEMNYTPERPDGSIDISKALQVNVEFDLSRQLWSYLVRQG
                     AITDPRSFIHPVPHMSFVRGAANIAFLRKRYAAMTAHHCYKGMEYTEDRNKIDKWAPL
                     IMEGRAADDKVAATRIITGTDVDYGSLTHHLVQRLVSQSGFAVNYRHRVVDIARGDSG
                     GWRVGVENMATGAVQSHMTKFVFIGAGGGALPLLQKSSIPEGRGYGGFPVSGIWLRCD
                     DPAIAQRHHAKVYGKASVGSPPMSVPHLDARIIGGQHSLLFGPYAGFSSKFLKHGSLL
                     DLFESIETSNIVPLLSVARDNFDLTEYLMGQLLLTPHQRFRTLQDYYPDAVQAEWRLQ
                     VAGQRVQIIKPDMDKGGLLEFGTELVAADDKSLVALLGASPGASTAAFIAISVLEKCF
                     SQELTASAWLPKLKEIIPSYGVSLIDDAVMTQNVRAETARTLNIEAV"
     gene            complement(15109..15504)
                     /locus_tag="VKQ27_20690"
     CDS             complement(15109..15504)
                     /locus_tag="VKQ27_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLY91412.1"
                     /translation="MIRCVRLWTGDDDNSHFEEGVLDFEQGARGDLLTSTMGATSISF
                     QETKSGGAFAWHTAPARQFVITLSGTLDFETRSGAHFILHPGDILYAEDTAGGGHRWK
                     LTDDQPWRRAYVILAPGIPVPFTPMGKIQ"
     gene            complement(15663..16919)
                     /locus_tag="VKQ27_20695"
     CDS             complement(15663..16919)
                     /locus_tag="VKQ27_20695"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HLY91413.1"
                     /translation="MAAYVAPMGDLAPISLLATLLFANGQTTERTIVDTERLAAALGH
                     QVSIIVRWGELIVRYTEPGATIRNEIVVVSPTGIDMHKVAAATSVIDDVCAHRIDAAA
                     ALAALASVAGQPPASLARFATLAAAGAAALGVIFGASHPLTLLLIAVSAGLGGCLRRW
                     LASVSHNAFLQPLSAALLAGIIGAVAARLDSSSALHLIAVCPCMVLVPGPHLLNGAID
                     LVRARMALGASRLLFATLIVLAICIGLLGGLAIGGITLPVMEASAPVPFGYDVIAAGV
                     AVGAYGTFFSMPWKTLPIPVVIGMLAHATRWAVITFGDASVATGALIACLLVGAIVTP
                     VADRMRMPFAAFSFASVVSLIPGVYLFRMASGFVSIASLGDQAPSSILLGTVADGISA
                     MLIIMAMTLGLIVPRMWIESCHPRGR"
     gene            17041..18765
                     /gene="poxB"
                     /locus_tag="VKQ27_20700"
     CDS             17041..18765
                     /gene="poxB"
                     /locus_tag="VKQ27_20700"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HLY91414.1"
                     /translation="MAKVADLFMDILHSAGVRRIYGVVGDSLNGMTEALRKRGDIDWV
                     HTRHEEAAAFAAGAEAHLTGNLAVCAGSCGPGNLHLINGLFDCQRSRVPVLAIAAQIP
                     GAEVGRNYFQETHPDSLFRECSHYCEFVSNPAQLPGVLETAIRAAVGHRGVAVIVIPG
                     DVALRDIDIAHSTGRSLTVPSPVVVPPDTELDALAALLNQSSRTTLLCGRGCAGAHDE
                     LLQLADTLKSPIVHALGGKEYVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLLMLGTDF
                     PYRQFYPASAKIAQVDLRPENLGRRTRLDLGLVGDVGTTIKALLPKLQAKQGRSYLDA
                     ALAHYRKAREGLDDLATGRPGRPPIHPQYLAKLLSEQASEDAVFTFDVGTPTVWAARY
                     LMMNGKRRLIGSLAHGSMANALPQAIGAQAAFPGRQVISFSGDGGFAMLMGDFLTLVQ
                     QKLPVKVVVLNNGVLGFVALEMKAAGFLETGTSLTNPDFAAMARAIGVHAVRVEDPGE
                     LSGAVKDVLAHDGPALLDVVSNPQELAMPPAIQLEQAKGFGLWALRTVMNGRGDELIE
                     LAKTNLLR"
     gene            complement(18965..19828)
                     /locus_tag="VKQ27_20705"
     CDS             complement(18965..19828)
                     /locus_tag="VKQ27_20705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLY91415.1"
                     /translation="MEAAEERGTDLTIFSPRAAGMGHHLGDASTSEAWATACNELIYR
                     VCTQFPRNFIGVCMLPQSAGVSPRNCIAELERCVTQYGFVGCNLNPDPSGGYWKDPPL
                     GDRYWYPLYEKMVELDVPAMIHVSAACNPAWHTTGSHYLSGDTAGFMQCLTSDLFKNF
                     PTLKFIIPHGGGAVPYHWGRFRGIAQDMKLPPLQELLLKNIFFDTCVYHLPGQELLAK
                     VIPAENILFASEMIGAVRGIDPETGFHYDDTKRYIEQLPFNLDQKAKVYEGNALRVYC
                     RLAAQLKKQFG"
     gene            complement(19795..19983)
                     /locus_tag="VKQ27_20710"
     CDS             complement(19795..19983)
                     /locus_tag="VKQ27_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91416.1"
                     /translation="MIIDCHGHYTTSPRQHEAWRTEQIAAHKEGRPAPARPAISDDEI
                     RDSIVNGQLKLQRNVAPT"
     gene            complement(20048..21553)
                     /locus_tag="VKQ27_20715"
     CDS             complement(20048..21553)
                     /locus_tag="VKQ27_20715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HLY91417.1"
                     /translation="MPDEGYQPTTAQVHVAHPPTARRTLVLAACLMATFMAAVESSIV
                     ATAMPTIVADLGGFNLFSWVFAVYLLTQAVCIPIYGRLADVYGRKKVFYFGAGLFLVG
                     STLCGLSRNMLMLILFRALQGFGAGGVQPIATTILGDIYTPTERAHVQGLVSSVFGVS
                     AILGPTIGAFLVEQVTWQFVFWVNLPIGVAAIAMIATFLTEPGEYSPRRIDYIGSLLL
                     MVSVAALMLVLVQGGSLPHATLAIVGGIALLSFITLIVHESSTPEPMLPLELWRHHRV
                     IVVGSLGGAVIAAVMTGVSAFLPTYVQGAMGRSALTGGLVLGAMSVTWAVASFYGGRL
                     MVRTTYRLTAVIGTAALVIGTFVLVLLTPERGVVWATIGSLLMGIGMGACNTTFIVSI
                     QASVPWHQRGAATSSIMFMRFLGQSLGAASFGAVLNFTLLRQLPGSVHLVDQLLDPET
                     RGTLPAGELQRLVGIIASGLHNTYVLACLLSLVSLGLALLLPARLSPTRQA"
BASE COUNT         4015 a         6792 c         6948 g         3840 t
ORIGIN      
        1 gaagcgcatt ccggggctga ataccgacag cctgcacgac tgcttcacgg atgcgagcgt
       61 atgggcgaat ttcgagcctg gctgcctgac gctgtgcagc ccagatcccc aggcgatccg
      121 catgccggat gacaagatca acgtgctgac ggtgacgctg ccgacgaact tcaaggcagc
      181 ccggttcgat tcggatgcgt ctaccaacat catgcggcaa ttccagcgag agatcgaggc
      241 gatccggttc aatgcgggcg acgcccagat cgatctgccg gtgaagctga aggtgcatga
      301 atcgatcttc gtgccgctgg cgaagtgggc gatgctgtgc accggcaact atcgctgcgt
      361 gaagaaggac gcggcggtgt cgatcaagga ggcggtacac accgatctgg cgacgtcgga
      421 acgtgtatac aactgggtgc gcgagctctg cattctgctg ggcgcgtcgc cggatgatct
      481 ggtgccgttc gagaagtatg ccgcggcggc caatgggctg atccggcctt cctcggcggc
      541 gcgggcgctg tttgccggcg cgccgaatat cgaacgcgtg gaccggctgg tgcagagcat
      601 cggcgcgcag cggggcatgc ggaatgccga actggatgcg acggtggcgc tggtggatgc
      661 gcggctggag atgaaccgga aggctgccgc ctgaaccctc ccgcggacgt ttcacgctcg
      721 cctcacgctg cctggccgag tattgtttgt acgaagccag gggaaacagg gggactcaga
      781 tgcctatgga gcgggtcgtc gtcaaaaact tcgaaatgtg gggccggctg gtcaagacct
      841 gggctaccgg cgacgattat gtccgcgatg gcaactcgta tccacgtcct gcggaccttg
      901 ctgaattcaa acggcaggtc cagcaggcga actgtgaaat gacgatgccg gatcgattga
      961 ccaagctcga catggtgcaa ggtgatgatg tgaccctcgt cgtccgcttg ccaccagcgc
     1021 agatgatcgt cgattcagag cacgtgctcg ccgagctcgg ttcggtctcc gtgggatcgc
     1081 cctatcccct gccgacgttc tatcgtgatg cgtggggcgt ccagcagcag atcaacctct
     1141 cggcctcgca gttgctgctg ttccacaacg agcgcatcgg cgagtatacc atcaacaact
     1201 gcatgtaacg cctaccagtt gccgtactgt gcggtgccag cgggcaggct ggcgccgcgc
     1261 agcgcgtccc gcagcgcctc ccagacagcg cgttcgctga tcggatagag atgcagcagc
     1321 cagcagacaa tcgtctcatc cgtcggctgc ctgtcgccac gccaggcacc gcggatattg
     1381 gccaggaaac gtcgtgcgct ctcttgcgct tcctgttggc ggccaagtcg atacaatgcc
     1441 gctgcacgcc atgccggcaa tgtctttatt gcgtccattg ccatgtccgc cgcctgcagc
     1501 gccccctcgt tatcgccact gaggtaggca atgcttacct gataggccca atgcgtccgg
     1561 cttggtcgaa gcgagcgatc gagcgcctgc cgcgccaggc cggtggcact ttcgaagcgg
     1621 ccgcggaatg cttcgaacag ggcgacggca atcagggtcc atgggtcgtg cggattcagt
     1681 tcgcctgcca tgtccatgtg caaatcggcc gaaccgaatt gtccggcgag cgcatgcgac
     1741 cagccgaggc aaagctgcgc acgggtgtca atcggatcga gctgaactgc cttgcgcgcc
     1801 agctcaagcg aacggagcgt gcggtccgga ctccgcaaga ctcccggatg cacgatatgg
     1861 atcgcattgt tcatttgcgc gaggctgcaa tacgcccccg taaagttggg cgccgccgca
     1921 atgatctggt tgaactcatg ctcggcccgg tgccaatctt ccgggctgaa agtcaggatt
     1981 tgcgactggc agcgcagcca acggtcgtag atgccgagtg cgacatcggg ttcagcggag
     2041 aaccgcatga tgcgttcggt tgacagatgt accttcagcg cagtcgctat gcgcgtgacg
     2101 atccgccgct gaagttcgaa ccagttttcc agcttcagct cgaatgtctc gctccatacg
     2161 tagatgttgc tgtccacctc cttcaggatc aggaccagac cgatgctgtc gcccgtctcc
     2221 tgtgccagga tattgatctc gtaggatgcg ctgacatccg atggctcatt ggcggctatg
     2281 agttgcggcg gctgatcggt cacgtaccac tcgcggaacc ttacgagaca ggcgatcaga
     2341 tgctggcgaa aaccctcgat gagatgcggt ttgtcagcag cgagcccccg gacatcgacg
     2401 acgccaaccg agagcacaag cttgctgcca gaccgcacgg caggtgcggc gcttaggttc
     2461 gccgcgatcg gaacggccgc cggctcaaac gcgccggatt tgacatcggc gaccaattgc
     2521 tgcgtgggcg cggagggctc catcccgtac tcgtcgtcca gcagatccca aagctgcttg
     2581 tacagcttga gtgcaccgcc ggtgtcgccg gcctcagccc gccactgcat cagtagccgg
     2641 cacgcctctt catgcgtcgg atccagattg atcgtcgctt cggccagccg gctgcgcgtc
     2701 ggacgatcga gatcgtcgcg ccgcatgccg gtttccagcg cccttatcag acgctgctgg
     2761 agactgtgcc gcttggccag caggtagacg cgaaactcag catcgatgtc gtccaggtcg
     2821 agcagcaagg tatcagcaag tctggcctgt ccgagcaaca aaggatgcgc tcgatgcgcc
     2881 tccgcctcct cgatcactga agcgagatcg acttcgacgc tggctggatc gagctcgatt
     2941 gtcagccggt tggcttgcag cccaccatat ccggcctcgc tgaatgcctg acggatctcg
     3001 cgcaatgtct ggcgaagcgt cgtgcgcgca cggtcctcat ctgagtcgct ccacagcaag
     3061 ccgaccagac gttcccgtgt ttcccgcgac gttggattga gcaccaggca gccgagcacc
     3121 gcgcgggcct tgcgtccgat caaccggaca ggccggccgc taaacgacag cgccatcggt
     3181 ccgaacatag agatgcaaag ccgcggccca gtccagcgtg gagtgggctc ggtcaggtcc
     3241 atgcgtagcg cggcgtctgc cattggcgtg gcacaccagg aagaacaagg tagcgatcat
     3301 cccttcgtaa tcagaacaat gcaacgctgg ccgtttccgc accaagttca cgacagcgca
     3361 tcatggcgtc acgcaagcga gcggacacga tggcacaggg atcgagctgc aatgccggag
     3421 cgatcacccg caccacggga acgccaaatt ccggccgcgt cagatcgatc gcgtagctct
     3481 cgatcccttg agccaccagc cgtgcaacca ggcgcgacag caaagcttcg tcgccatcag
     3541 cgccctcatc atcatgcgcc aagccagtgg gtggtcctgg atgcagcagc ctgcaagcgg
     3601 acgtatcgat cgcctgcgcg cgcctcaaat gggctcgatc ccccgtaccg agggcagatt
     3661 cgccattctc tcgcaatctg gactgcgcaa gatgcagccc gagctccatc tggcacaatt
     3721 cgaacagcgc ggctgtcgcg gcggcagcca tcgtgggacg acaggcgaag cctgcggcaa
     3781 agccgtagcc gtcgtggcgc gccgaaacgg cggccacgac cgggacgccc aggtctgtcg
     3841 tgatatccag caaccaggtc cggcgtgacg aggcggttcc ccgcaatcgc tctatttgaa
     3901 gcgcggcgtc gcgcgaggcg gcccactcca gcggcaccag gtgacctcgc acaccgccgc
     3961 gccaccattg tgccgttgcg tcgcgctcca ccagttccag cagtgcgtgc agcgtggcac
     4021 cggtcgctga tacgccggcg gcgcacccga cgctcagccg cagcggtggt gaaaagtctt
     4081 gagcttcggc gattcggcgg acacagagat cgaggggcat cggtgcctcc cctcccccat
     4141 gcaggcgcgc cgcatgggtc catgcgattg gccgactgcg agacaccccg cagtgatcca
     4201 acaacgcgcc gatgaaacgc tccgccggag cgtccagcag cactgctcgc tctacgaacg
     4261 atccaacatc ccgattggta tcgctcgcct cgatctgcga cagatattcg atcgcctcgc
     4321 ccacacagga ttcgaacgcc cgcctccaat ccacgccgga gccggcgaag ctggcgattg
     4381 ctgatcccgc accgggtgtt tctgcttcac cgccgaagaa gcacagtccc ggagcgtcgg
     4441 gcgcctcaat gcgaaacagc cgacgcagtc tcgccgcagc acgaagcagc gagacgcggt
     4501 ggcgcgcggc catggcgtcg ccgtccagat aacccaacag cgacaggagt tcagcggcat
     4561 cgggtggcgg cacgcccgcc tcccccactg aagttgccgc gcgttcaaac agagcctgca
     4621 ggccgtccat tgctttgccg ctgttatccg cgagtccggc ccgtcgatac aagcgcgtcg
     4681 gtgagctcag cgtgaatcgc gtccgcgcga tgggttcacg cggatcaggt cagactgcct
     4741 acaaaacgac ggcgaggcaa ccgggagctt cgtcagtgca atcccgcact ggaggttttg
     4801 cggccatgcc gtttcccggc tcgtccttgc ccctgttcga cgtcggcacg ctgttcgcgc
     4861 tggtgggacc accagccgga agcagctcgc ccgcccgccc gcatgatcgt ccgatcgcgg
     4921 tcctcggcgg ctgtctcgct ctcggctgct atattgcgat gatcgcacac ttaggcctgg
     4981 cgccacacgg cgtagccccc atcatagccg tcttcatcgc ggctcttgcg tcgagcgtcg
     5041 tggggtttgc gttctccgcc gctggcggcg cgatcctctt tcatgtgctt ccgtcacccg
     5101 tcgagatcat ccagatcctg cttgtttgca gcattgcgaa ccaggcgctg agcgtatggg
     5161 ccctccggcg tgccatcagt tggcgtgcca cagcaccgtt cctgctgtcc gggatgctcg
     5221 gcgcaccggc gggcgcctgg ctgttgcttc acagcgagcc gcacgcgtac ttgctcgcat
     5281 tcggtgttct gctcatcggc tacagcctga gcgcactgat acgaccgcaa acccaactgc
     5341 gccggcgatg gctgggcgga gactggctgg tcggcttact gggcgggctc accggtggat
     5401 tcgcagcatc gcctggcgca cccctgtcga tctggctgac gttgctgggc tgggacaaat
     5461 tgcggcggcg cgcggtgtat cagcctttca tcttggtgat tcagtgtgtc gcgctggtgt
     5521 cgatgcaggc gatggcacgg caggtccagc cacagggctc tattacaggc gtcagcctgt
     5581 acctgccggc gtcgctgctg ggcacctggt acggtctgtc gatcttcgca gccctcaacg
     5641 acaaccagtt cacccttgcg atcaacctgc tgctgatcgt gtccgggttg gccctcgtcc
     5701 tgtgatggag aatatcatta tgcttgaagg attcatgata gctgattcag gctcgatcgc
     5761 gagccgtgag acaccagctc ctgacgcgtc ggcgagcatc cttccatttc cgcctcgcat
     5821 catccgtgcc ccacgggata tggcgttgct gctgatgcag gtatcccctc tggtggcccg
     5881 gcgcttcgcg gttcagatgc acgagcgcgc ctgccacggc acggaccaga cctgggccga
     5941 tttctggctg cacgtgatcc atcagctcgt gacgccgggc aacatggagg gcgcaaagtg
     6001 accgcgccca tcctgaagtc ctggctcatg ttgtttggct gtctcgccgc aggcgaaact
     6061 ttgcatcgga tcgccggcat tcatgtgtca ggtgccgtaa ttggcatggc catactcgtt
     6121 gcagcactca cggtcaccgg tggaccatcc gaaccattcg aacaagctgc gagcagtctg
     6181 ctcaagcact taccgttgct gttcgttccg gcaggggttg gcgtgattca gcaagtaacc
     6241 ataattcgca gccaaatcct gcccattgcc attgcattgc cggtgtcgac cgccttcgcg
     6301 atggcaattg ctgctgttgt cgtggaacgc atgactgcgc gggagccgct caaagagcgt
     6361 gttctgcaga tggaggagca atgatcaccc cgatcgcccg gcaatggatg accgatgcaa
     6421 tgcagccggc aacgtggatg acgctgacgg tcctcgctta tgtgctcgca atagagttgc
     6481 agcgcctatg caaagcgacg ccgctgttga atccgacatt gttgtccatc gcagccgtca
     6541 tcgccgttct gctgctgacg cgctcgaact acggagcgta ttttcgcgct gtcgagccga
     6601 tacatcttct gcttggcccg gcgacagtag cgctgggcct gccgctgtac aagcagcttc
     6661 atgtcattcg tgccaatgcg atgcccgtca tggccggcgt catcgtcggc gctgtcatcg
     6721 cctgtggcag cgcagtcgcc attgcgtggc tacttggggg ctcgccggac ctgctgctct
     6781 ccttggcacc gaaatcggtc acggcgccaa ttgccatgag catttcgcag gagatcggtg
     6841 gatcgccaac gctgactgcc tcactggtga ttacgaccgg catcattggc gcggtgttgt
     6901 gccgatggct gtttgattgg gtcggggtca agagctggcc cgcgcgagga cttgcaacgg
     6961 ggatcgctgc gcacggcatc ggcacaggcg caattctggc aacgaacgag gtcgctggcg
     7021 cgtttgccgg cctcgccatc ggcctgacag gactcttcac cgccatcgtc ttgcctgtcg
     7081 tggcaagctg gttccccgga taatgctgct tcaggcgcga ttccgctgcc tcaaggcccg
     7141 ccatctcggg cccatcatgc gctggaactc ccgcaagtcc cacgcaccaa agaggtgcgc
     7201 tgcagcgacg taagcaagca gtccgcctgc taccagaacc gccagagcct caaatctggc
     7261 cacgccgtgc ggcggcgtcg cgaacagcat ccgctgaatg ataatcagca cgactgccat
     7321 cgcacccgcc gcggcgatca tgcgcagacc acggcgacgc agttgcgcat ccagccgaag
     7381 gtggccacgc cgcaccagca cccacgcaaa ccagccggta ttgaacatcg cggcgaggct
     7441 ggtcgccagc gcagggccaa tgtgctgcag cggcaccatg aaggcgacgt tcattgccag
     7501 gttcagtgcc acggcggcga tgccgatctt cacaggcgtg gatgtgtcgc ctcgcgcgaa
     7561 gaagccggga gccagaactt tcaccagaac gaaggcgggc aggccgatgg cgtatgcagc
     7621 gagcgattgt gcggaaagcc tggcgctttc ggagttgaat gcgccacgcg cgaacagcac
     7681 ccacatcact ggataggcag tcacgatcag cgccacggct gccggtagcg tcaagaacag
     7741 cgcgtattca atggcgcggt tcagcgtgcc gagcgcggcg tcgcgctcgc cggcgcggac
     7801 ctggcgcgac agtagaggaa gcagcgcggt gccgaccgcg gtgccaatga ctccgagtgg
     7861 cagttgctgc acgcgatcgg catagtagag cagcgacacg gtgccggcag gcagcaggct
     7921 ggcgataatc acatccaccg cgagattgag ctgtgtgacc cccgcgccga tcaggcctgg
     7981 cagcatgcgc cgcagcagca cgcgaatctg cggcgtgagg cgcgggcgcg gaaggcgcag
     8041 cgccatgccg gcgttgcgca gtgcccacat cagcagccca agctgtgcga cgcccgacgc
     8101 ggtgacgccc caggcgagcg cgtggccggc ggtggggacg tagggcgtca gccacagcat
     8161 gcaggcgatc gaaatgatgt tgaacagcac ataggacgca gatgcggcgg tgaaccggtc
     8221 gagcccgttc agcgcgccgg ataccagggc cgcgaggcag atcagcagca gatagggaaa
     8281 cgtgatgcgc gacagcgcga cggtgagcgt gaacttcgcc gggtcggcgg agaagcctgg
     8341 tgccagcaca gtcatcatct gcggcatgaa gatctcgccg atgatggtga gcacgccgag
     8401 ccagaatgcc aggacggcga acgtttcttc ggcgaagcgg tgggcggagg ggcggccctc
     8461 ggcggcaagc ataccggcga attcggggac gaaggcggcg ttgaacgccc cctcgccgaa
     8521 caggcggcgg aaaaggttgg ggagtttcag cgcaacaagg aaggcgtcgg cgatcgggcc
     8581 ggtgcccagg atcgccgcga tcagcatgtc gcgggcgaag ccgaggatgc ggctccccat
     8641 agtccaaccg ccgacggtga gcacgccgcg cagcattatt cgtcgcgcct ggggcgggag
     8701 agcagcgcag ccatggcttc cttgagcgtg gtcgcgccgc tgaggagtgc cgcgaccgcg
     8761 gtgcagatgg gaaggtccac gccggcggcg cgggcaacca gggcaggggc agtggcaacg
     8821 ccctcggtga ccgcgctgcg ggctgcgagc acatcggcca gcacctcgcc acggccgagg
     8881 gcgaggccga ggctgaagtt gcggctggca gggccggtgc aggtgagcag caggtcgccc
     8941 aggccggaca ggcccatcac ggtctcgcgg cggccaccga gtgccgcggc caggcgggcg
     9001 agttcggcga ggcctcgggt gatgagtgcg gcacgggcgt tctcaccgag gccggcgccg
     9061 atgacggtgc cggccgcgat cgcgatcaca ttcttggcgg cgccgccgac ctgggcaccg
     9121 gtggggtcat cgttgccgta gaggcgaaag gagggggtgc taagggtggt cgccacgtgt
     9181 tcgcgcagga ttgggtcggt agcggccacc acagaagccg cagggaggcc ggctgcgatc
     9241 tcgtgcgcga agttggggcc ggtgaggacc gcagccggag tgccagggcg caactcctca
     9301 atgatctcca aaggcagacg cagcgttcct gtctccaaac ctttggcgca ggcgaccaac
     9361 ggtaccaagc ctggcggcaa ctgcggcagc acggtgcgga tgtgctgcat cggcacggcc
     9421 agcaacgtga tgtccgccgc agcgggggca gcgcctgtca ccagaatcgg ctcggggata
     9481 cgaacgccgg gcagacgcgc attttcccgg gtcacggaga tcattccggc acgctgctga
     9541 tcgcgcgccc acaaactaac atcgttgccg gcacgctccg cctgaatcgc gagcgctgtg
     9601 ccccaggctc cagcgccaat gactgcgacg ctactcattc gtgatccgtg tgatggtggc
     9661 gtactcacct ggctcgcgat cgcccaaccg cgcgagcagc gcttcgagta tcggccgaac
     9721 ttcttcctcg aagcgatacg gtgggttggt cacgagcaaa ccgcaaccgt tgagcctcgc
     9781 cgcatcgaca ggttcgcgca ggcacaattc cgccgacacg atgtcgcgaa tgccgctttg
     9841 ctgcatgccg gacaggaatt gccggaccgg tgcccgctgc ttaattggat accacgcgac
     9901 gaacacacca ttggcgaagc gcgcgtgaga ggcggcaaga ccacgcccga cctgggcgaa
     9961 ctcgtctcgg tcctcgtacg gcgggtcgat cagcacaagg ccgcgccgct ccttcggcgg
    10021 aaggagtgcc ccaagtgctt cccacgcatc gcgctgatgc accgccacgt ggcgatcacg
    10081 cgcaaaaagc tggcgcagcg cggaggcgtc ttcggaatgc agttcgcagc acgcgagacg
    10141 gtcgtcgggg cgcagcaacg cgcggatgag acaaggtgag cccggataat gcgcggcttc
    10201 ccatcgatcg tcaggccacc cctcgtcatg gccggccatc gagccggcca tctggctgtg
    10261 ctggtccact gacgcggcca ccgcggacgg tggagcccgg accgcgtcca gatagtcccg
    10321 cagcaccgcc ggcggatcat ccagcagccg tgcgataccg ttgcgccatt cgccagtgcg
    10381 cgctgccggt ccggtggtca ggtcgtagcg gccaacgccc gcgtgcgtgt cgagcacaaa
    10441 gatcggcgcg ggcttgcgtt gcaatgcgcg caacagccag accagcacgg catgcttcac
    10501 gcagtccgcg aaattgccgg cgtgaaaggc gtggcggtag ttcacccgcc gcttatggca
    10561 gccccgttga gtcgggtcga ggacaacggc gtatcaatgg aacgtgcggc cggagtcgat
    10621 gacgatggtc tggccggtca tcgtttcggt gcggcacatc gtgaccacca tgtcggcgca
    10681 gtcgtccttg tcggcggcct tcttcagcag cgaggacgag gcggaacgct cgatcatctc
    10741 ggggcgcaga ttggcggtgg cgcgggttcc ttccagcaaa cctggggcga cacagttcac
    10801 cagcgtctcg ggcgccagtg ccacggccat gcagcgcgtc aggtggatca ggccggcttt
    10861 cgacactgca taggcgatcg acgacccctg cggccccagt ccggcgaccg acgagatgtt
    10921 gacgatacgc cccctgccct gccgcttcat gatcggcgcg atggccttgg tcaggcgcat
    10981 cggcccggtc aggttcaccg ccatgatctt gtcccacaca tcctcggtga ggctgtcgag
    11041 gtcggtgaac gggatcgcct tattgtaggc ggcgtcgttc accaggatat cgatgcggcc
    11101 gaagcgctgg gcgacgtcct gcaaaagctt ggcgatggcg ccgctgtcgg tgatgtcgca
    11161 tgggaaggcg gcggcattga tctggtagct ggtagcgagt tcgcgcgcga cgccttcggc
    11221 ctgctcgcgg ctctgggcgt acatgaccgc gacatggaca ccttccttgg ccagcgcgtg
    11281 gcagatgcgc tgcccgagcc caccattgcc gcctgtgacc agggctaccg cgcctctgag
    11341 ttccatgaac tgtctccctc gattgagagc agtttgctgg gagggcggac agggggcaag
    11401 ccgttgcagg cgatcacggt accagtcgca ctgtcattgc gagaagcacg gcggacgaca
    11461 caatccgaat ccaagtcgtg ccaatcttgc caaggtctga ttcagagcac tcggtgagaa
    11521 ccagcccatg cacacgatgg tcgcaatccc cacgccgcag tctacgctcc gctaggcggc
    11581 acgtaacgct caaccacgat ctccccttcg cgctgacgca taatgtagct gtcgtaccat
    11641 gcctgcatgc acaacagcgt atccatgttg atgccgaacg ccttctcgat gcgcgcagcg
    11701 ccatttcggg cgacagtccc gctttgccgt tcaccaggtc cgatagcgtg gctcgccgca
    11761 cgcccaacaa agccgctgcc cacgccactg gcagacccag ttccgcaagg atttcctcgc
    11821 ggatgaattc gcccggcgag gacagccgca tgccaagctt gattggtgcg tcatccatca
    11881 gtgatagtcc tccctccagg ttccgatcac tgatctcccc ttcttcaagg gcgaacgtga
    11941 cccgccggtt gccagatgcg gaaatgctcc aggtggccgc ccggtcgcag gtaagctggt
    12001 ggattcgcca actaggcggt ccccggacgc cgtccatgtc gggaccaaag aacagcacag
    12061 tcagaatacg cgaagccgat tgagcaggtc gggtcgagcc gccagtcaaa cgactcgaac
    12121 gggaaacggc ggtcgcgaaa ggtcggccct gactcgttgc gaagcgcaac gtgctgtctc
    12181 agcggcagca ggcaactcag ctagcagtag caacgtaagg ccgcaccatc ttcgatggat
    12241 ccaccacgcg gttgaactcc tgttcggtga cgaaacccag cttcagcgcg gcctccttca
    12301 gcgtcaggtc atgatccatc gcgtagtgag cgatctgcga ggccttgtcg tagccgatca
    12361 ccggcgccag cgccgtcacc agcatcaacg agcgctcgac gtattcctcg atcttcttca
    12421 ggttcggctt ggtgccttcc accaggaact tgcggaaatt ggtgcagccg tcggtcatca
    12481 gcgtgatcga ctgggtgatg ttgctgatga tcagcggctt gtagacgttc atctccagat
    12541 agccgccggc gccaccgaac cccaccgcga cgtcgttcgc catcacctgg acggcgagca
    12601 tggtgagtgc ctcggcctgt gtcgggttca ccttgcccgg catgatggag gagccaggct
    12661 cgttctccgg gatcagcagc tcggcaaagc cggcgcgcgg accgcaggac atcaggcgga
    12721 tgtcgttgcc aatcttgtag agtgacgtcg ccagcgtgcg cagcgtgccg gaaagctgca
    12781 ccaacgcatc atgcgcgccc tgcacggtga acttgttcgg cgcgctgatg aaaggcagtt
    12841 tcgtgtagag cgcgatttcc gcggtggccg cctcggcgaa gccaggggcc gcgttgatgc
    12901 cagtgccgac ggctgtaccg ccgagcgcca gcttgtatac acccttcagc gcatcatcga
    12961 tccgctccac atcatccgac agcatgccgg cataacccga ccattcctgg ccgagcgtca
    13021 gcggggtcgc gtcctgcatg tgggtgcggc cgatcttgac gatgctgtcc cattccttcg
    13081 ccttggccgc gatcgcgtcg tgcagcccct tcaccgcagg caccagccgg ctggtcacgt
    13141 tgaccgcggc ggcaatgtac atcgcggacg ggaacgaatc gttcgacgac tgcgacatgt
    13201 tcacgtggtc gttcggatgc accggcgcct tgctgcccag agcggtgccg gcaagctggc
    13261 agcaacggtt ggagatcacc tcattcacgt tcatgttgaa ctgcgtgcca ctgccggtca
    13321 tccagacgtg cagcgggaac atgtcgtggt gctggccggc gaggatctcg tcgcaggtct
    13381 tgatgatcag gccgaattcc ttgtcgtgca ggcgaccgcc gttatggttg gcgatcgcgg
    13441 cggaacgctt cagggtcgcg taggcggtga tcatctcgcg cgggatcagg tcgtggccga
    13501 tgctgaaatg ctccagcgag cgctgggtct gcgcgcccca caacttgtcg gccggcacat
    13561 cgaccgcgcc catgctgtcg gtctcgcggc ggaattcggt catgcctggt ctcctgcttc
    13621 agacagcttc aatgttgaga gtgcgtgcgg tttcggcacg gacgttctgc gtcatcacag
    13681 cgtcgtcgat cagcgacacg ccataggatg gaatgatctc cttcagcttc ggcagccagg
    13741 cgctggcggt caattcctgg gagaagcatt tttccaggac gctgatcgct atgaatgccg
    13801 ctgtcgatgc gccaggtgac gcgccgagaa gggcgaccag cgacttgtca tcggccgcca
    13861 ccagttccgt gccgaattcg agcaaaccgc ctttgtccat atcaggcttg atgatctgca
    13921 cgcgctgtcc ggccacctgc aatcgccact cggcctgcac ggcgtccgga tagtagtcct
    13981 gcagcgtgcg gaagcgctgg tgcggggtca gcagcaactg gcccatcaga tattcggtca
    14041 ggtcgaaatt gtcgcgcgca acggagagca gcggcacgat gttgctggtc tcgatcgact
    14101 cgaacaggtc gagcagcgag ccgtgcttca ggaacttgct ggaaaatccc gcatatggcc
    14161 cgaacagcag cgagtgctgg ccgccgataa tgcgcgcgtc gagatgcggc accgacatcg
    14221 gcggcgaacc gaccgaggcc ttgccgtaga ccttggcgtg atgacgctgc gcgatggcag
    14281 gatcgtcgca gcgcagccag atgccgctga cgggaaaacc gccataacca cggccttcgg
    14341 gaatgctgga tttctgcagc aatggcagcg cgccaccgcc cgcaccgatg aacacgaact
    14401 tggtcatgtg cgactgcacc gcgccggttg ccatgttctc gacaccgacg cgccaaccgc
    14461 cgctgtcgcc gcgggcgata tcgacgacgc gatgccggta attcaccgcg aagccagact
    14521 ggctcaccaa gcgctggacc aggtgatgcg tcagcgagcc gtaatccaca tccgtgccgg
    14581 tgatgatgcg cgtcgccgca accttgtcgt ccgcggcgcg gccttccatg atcagcggcg
    14641 cccatttgtc gatcttgttc cggtcctcgg tgtactccat gcccttgtag cagtgatgcg
    14701 cggtcatcgc cgcgtaccgc ttgcgcagga atgcgatgtt cgcggcaccg cgcacgaagc
    14761 tcatgtgcgg caccggatgg atgaacgagc gcggatcggt gatcgcgcct tgcctaacca
    14821 gatacgacca gagctggcgc gacaggtcga attcaacgtt gacctgcagt gccttggaga
    14881 tgtcgatgct gccgtcgggc cgttccggcg tatagttcat ctcgcaattg gcggcgtggc
    14941 cggtgccggc gttgttccac gcctcggagc tttcctgcgc gcagtctggc atcgcttcga
    15001 acatcgcgat ggtcagtgac ggctggagtt ccttgagcag cacgcccagc gtggcgctca
    15061 tgataccggc gccgatcagg atgacgtccg gcgtatcgat ggattcgctc attggatctt
    15121 tcccatcggc gtgaatggta ccgggatgcc gggagccagg atcacatagg cacggcgcca
    15181 cggctggtcg tccgtcagct tccagcggtg accgccgccg gcggtgtctt ccgcatagag
    15241 aatgtcgcct ggatgaagga tgaaatgcgc acctgaccgc gtctcgaaat ccagcgtgcc
    15301 actgagcgtg atcacgaact gccgggctgg cgccgtatgc caggcgaagg cgccgccgga
    15361 cttcgtttcc tggaatgaga tggacgtggc gcccatcgtg ctggtcagta gatcgccccg
    15421 cgcgccctgc tcgaagtcca gtacgccttc ctcgaagtgg gagttgtcat cgtcgccggt
    15481 ccacagccgt acgcatcgga tcattcggga cctccatcac tcggccacaa acctatcggc
    15541 atgttccccc cagctctgtc acattcgggc gggaatttgc caatttccat gagcgaacga
    15601 taccaaacta attgcattgt ccgagcgaga accgtgattc ctccgtaaag atcgcgttta
    15661 tgttatcgcc ctcgcgggtg gcacgattcg atccacatgc gcggaacgat cagccccagc
    15721 gtcatcgcca ttataatcag catcgcggag atgccatctg ccaccgtccc aagcagaata
    15781 ctggatggcg cctgatcgcc gagcgacgct atcgagacaa acccgctggc catgcggaag
    15841 aggtacacgc ctggaatcag tgaaaccact gacgcgaagg aaaacgccgc aaacggcatc
    15901 ctcatccggt ctgccacggg cgttacgatt gcgcctacca gcagacacgc gatcagggca
    15961 cccgtcgcga cgctggcatc gccgaaggtg atcacagccc atcgtgtggc gtgggcgagc
    16021 atcccgatga ccacaggaat cggcagggtc ttccatggca tggagaagaa tgtgccgtag
    16081 gcgccaacgg cgacgccggc agcgatgacg tcatagccaa acggcaccgg agccgaggcc
    16141 tccatgaccg gcagggtgat cccaccgatt gccagaccac ccagcagtcc gatgcagatc
    16201 gccaggacga taagcgtggc gaacagtagc cgcgaggcgc cgagcgccat gcgtgcacgg
    16261 acaaggtcga tcgcgccatt cagcagatgc ggtcccggca ccagcaccat gcagggacac
    16321 accgcgatca ggtgcagggc ggagctcgaa tccagccgtg ccgcgaccgc cccgatgatt
    16381 ccggcgagca gtgccgcaga aagtggttgc aagaaggcat tgtggctgac gcttgcgagc
    16441 cagcggcgca ggcagccacc gaggcctgcg ctgaccgcga tcagcaacaa ggtgagagga
    16501 tggctggcgc cgaatatcac cccgagtgcc gctgcacccg cggctgccag cgtcgcgaag
    16561 cgcgccagcg aggctggcgg ctgcccggcg acgctcgcca acgccgccag cgccgccgca
    16621 gcgtcgatgc ggtgcgcgca cacgtcatcg atgacgctgg tcgccgcggc gaccttgtgc
    16681 atgtcgatac cggtcgggct gacaacgacg atttcgttgc gtatggttgc accaggttcg
    16741 gtgtagcgga cgatcaattc gccccatcgt acgattatcg acacttgatg gcctagcgcc
    16801 gccgccaggc gttcagtgtc gacgatcgtg cgctccgtgg tctgaccgtt cgcgaagagc
    16861 agcgttgcta acagagagat cggtgcaagg tcacccatcg gtgcgacata ggccgccacg
    16921 ccgggagccg caacgtcgct ggaatttggc ggcgcagcgg atggctgagc aggaacagac
    16981 aatacgatag tggctgatcg tggccacgac gcgagcttcg cagcccagga gacaagtccg
    17041 atggcaaaag tagccgacct gttcatggac attctgcatt cggcgggcgt gcgacgcatc
    17101 tatggcgtgg tcggcgacag cctgaacggc atgaccgaag cgctgcgcaa gcgcggcgac
    17161 atcgactggg tccatacgcg tcatgaagag gccgcagcgt tcgccgccgg tgccgaggca
    17221 cacctgaccg gcaacctggc cgtctgcgcc gggagctgcg ggcctggcaa tctgcatctg
    17281 atcaacgggc tgttcgactg ccagcgttcg cgtgtgcccg tgctggcgat cgcggcacag
    17341 atccccggcg cagaggtcgg tcgcaactac ttccaggaga cgcacccgga ctcgctgttc
    17401 cgcgaatgca gccattactg cgagtttgtg tccaatccgg cgcaactgcc tggcgtgctg
    17461 gagaccgcaa tccgtgcggc ggttgggcat cggggcgtgg ccgtcatcgt cattcccggc
    17521 gacgttgcgc tgcgcgacat cgatatcgcg cattcgaccg ggcgttcgct gacagttccg
    17581 tcaccggttg tcgtgccacc ggacaccgag ctggatgcgc tcgccgcgct gctgaaccaa
    17641 agttcgcgca ccacactgct gtgcgggcgc ggctgtgctg gggcgcatga tgagctgctg
    17701 caacttgccg atacgctgaa gtcgccgatc gtgcacgcgc tgggtggcaa ggagtatgtc
    17761 gagtacgaca acccgttcga tgtcggcatg acggggctga tcgggttcag ctctggctac
    17821 catgcgatgc tcagctgcga caccttgctg atgcttggta cggactttcc gtatcggcag
    17881 ttctatccgg cgagcgccaa gatcgcacag gttgacctca ggccggagaa tctaggacgg
    17941 cgcacacggc tcgatctggg gctggtcggc gatgtcggga cgacgatcaa ggcgctgctg
    18001 ccgaagttgc aggcgaagca gggccggtcc tatctggatg cggcacttgc gcactatcgc
    18061 aaagcgcgcg aagggctgga tgatctcgca accggccggc ctgggcgtcc gccgatccat
    18121 ccgcaatatc tcgccaagct gctcagcgag caggcgtcgg aagatgcggt gttcaccttc
    18181 gatgtcggca cgccgacggt ttgggcggcg cgctatctga tgatgaacgg caagcgtcgt
    18241 ctgatcggat cgctcgccca tggctcgatg gcgaacgcat tgccgcaggc gatcggtgca
    18301 caggcggcct ttcctggccg gcaggtgatc agcttttcgg gcgatggcgg gttcgccatg
    18361 ctgatgggcg atttcctgac gctcgtgcag cagaagctcc cggtgaaggt ggtagtgctc
    18421 aacaatggcg tgcttgggtt cgtcgctctc gagatgaagg cagcggggtt cctggagacc
    18481 ggaacgtcgc tgaccaatcc ggattttgcc gcgatggcgc gcgccattgg cgtccatgca
    18541 gtgcgtgtgg aggacccggg cgagctctcc ggcgcagtca aggatgtgct ggcgcacgac
    18601 ggaccggcgc tgctggacgt agtgtccaac ccgcaggagc tggcgatgcc gcccgcaatc
    18661 cagctggagc aggcgaaggg tttcggcctg tgggcgctgc ggaccgtgat gaacggacgt
    18721 ggcgacgaac tgatcgagct ggcgaagacc aatctgctgc gttgaacagg gaatcgtgct
    18781 gacgcatctc atgtcaccgc agcacccaca ttgagtggat agcgttgaac ttgtggataa
    18841 aggtcaacgc tttctctgtt ggcgcgccac ttcagtctgc aaggcacctt cctgccatgc
    18901 gagacgatct acatcggtgc agtgacgcgg tggaacaccc gcgttggaca attgcaatga
    18961 cagcctatcc gaactgcttc ttcaattgag cagccaagcg gcagtaaacg cgcagcgcat
    19021 tgccctcgta gaccttggct ttctggtcca ggttgaaggg cagctgctcg atgtagcgct
    19081 tggtatcgtc gtagtggaag ccggtctccg gatcgatgcc gcgcactgct ccaatcatct
    19141 ccgatgcaaa caggatgttc tcggcgggga tgaccttcgc caggagttcc tggccgggga
    19201 ggtgatagac acatgtatcg aagaaaatgt tcttcagcag cagctcctgc agcggcggaa
    19261 gcttcatgtc ctgcgcgatg ccacggaacc ggccccagtg atacggcacc gcgccgccgc
    19321 catgcgggat gatgaacttc agggtcggga aattcttgaa cagatcggag gtcaggcact
    19381 gcatgaaccc ggcagtgtca ccgctgaggt agtgtgaacc ggtggtgtgc catgcgggat
    19441 tgcacgccgc actgacatgg atcatcgccg gcacatcgag ctccaccatc ttctcgtaca
    19501 acggatacca gtagcggtca ccgagcggcg ggtccttcca gtatccgcct gacgggtcgg
    19561 ggttcaggtt gcagccaacg aagccatatt gcgtgacgca gcgttccagc tcggcgatgc
    19621 agttgcgcgg tgagacaccg gcactctgcg gcagcatgca cacgccgatg aagttgcgcg
    19681 ggaactgggt gcagacgcga tagatcagct cgttgcaggc ggtggcccag gcctcactcg
    19741 tggaggcgtc gcccagatga tggcccatcc cggcggcacg cggtgagaat attgtcaggt
    19801 cggtgccacg ttcctctgca gcttcaattg gccattgacg atgctgtcgc ggatttcgtc
    19861 gtcgctgatg gctgggcgag caggagccgg gcggccttcc ttgtgcgcgg cgatctgctc
    19921 ggtgcgccat gcctcgtgct ggcgcggcga cgtggtgtaa tggccgtgac agtcgatgat
    19981 catggttgcc tcccgttctg tgcgcggcga ctgtaagcac accggtccgg cgtacggaag
    20041 ccgccggtca ggcttggcga gtcgggctca atcgcgccgg caacagcagc gccagtccca
    20101 gcgacaccag cgacagcagg caggcaagca cgtacgtgtt gtgcaggccc gacgcgatga
    20161 tgccgaccag gcgctgcaac tcaccggccg gcagcgtgcc gcgcgtctcc gggtccagta
    20221 gctggtccac aagatgcacg gagcccggca actggcgcaa aagcgtgaag ttgagcacag
    20281 cgccaaacga cgccgctcca agcgactggc cgaggaagcg catgaacata atggatgacg
    20341 tcgcagcacc acgctgatgc cacggcactg aggcctggat ggacacgatg aaggtcgtgt
    20401 tgcacgcgcc catgccgata cccataagca atgagccgat ggtggcccac acgacccctc
    20461 gttcaggcgt cagcagcacc agcacgaatg tgccgatgac cagggcagcc gtgccgatca
    20521 ccgcggtcag ccgatacgtg gtgcgtacca tcagccggcc gccatagaag ctggcgacag
    20581 cccacgtcac cgacatggca cccagcacca ggccgccggt gagcgcgctg cgtcccatgg
    20641 cgccctgcac ataggttggc aggaaggccg atacgcctgt catgaccgcg gcgatcaccg
    20701 cgccaccgag gctgcccacc acgatcacgc gatgatggcg ccagagctcc agcggcagca
    20761 tcggctcggg tgtgctggac tcatgcacga tcagcgtgat gaacgacagc agcgcgattc
    20821 cgccaacgat ggcgagcgtc gcgtgcggca ggctgccacc ctgcaccagc accaacatca
    20881 gcgcggcgac cgagaccatc agtagcagcg agccgatata gtcgatccgg cgtgggctgt
    20941 actctcccgg ctccgtcagg aaggtggcga tcatcgcgat ggcggcgacg cctatgggaa
    21001 ggttgaccca gaacacgaac tgccaggtca cctgctcaac gaggaacgcg ccaatcgttg
    21061 ggcctaggat cgccgacaca ccgaacacgc tcgagacgag gccttgcacg tgcgcgcgct
    21121 cggtaggcgt gtagatgtcg cccagaatcg tcgtggcgat cggctgcacg ccgccagcgc
    21181 caaagccctg cagcgcgcgg aacaggatca gcatcagcat gttgcgcgac aggccgcaca
    21241 gcgtggagcc gacaaggaac aggccggcgc cgaagtagaa aaccttcttg cggccgtaga
    21301 cgtcggccag ccggccgtag atcgggatgc agacggcctg ggtcagcaga tagaccgcga
    21361 agacccagga gaacaggttg aagccgccga gatccgcaac gatcgtcggc attgccgtgg
    21421 cgacgatgga gctttcgacc gcggccatga atgtcgccat caggcaggcg gcgagcacca
    21481 atgtgcggcg ggccgtgggc ggatgggcaa cgtggacttg ggcggtcgtt ggctgatagc
    21541 cctcgtcggg cacaggctcg cctcgttatg gttgggacgt ggcgggggtt gtgtc
//
LOCUS       DATGEM010000669         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_618608, whole genome shotgun sequence.
ACCESSION   DATGEM010000669 DATGEM010000000
VERSION     DATGEM010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618608"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VKQ27_20720"
     CDS             complement(<1..329)
                     /locus_tag="VKQ27_20720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLY91418.1"
                     /translation="MPYPDNEWLTDGVRTRRIFAWFVDLLLICLIVAGLWFVLLLFGL
                     LTLGLGFPLMGILPFIPFCYHFLFVAGSGATPGQQAMGLAVRRDDDLGPPTALQALIY
                     TLVFYLTL"
     gene            complement(338..2632)
                     /locus_tag="VKQ27_20725"
     CDS             complement(338..2632)
                     /locus_tag="VKQ27_20725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLY91419.1"
                     /translation="MQSLYRFLRRSLVGVGFLILLAAALVTGLLWLSLPQRQQQARIP
                     GLTGPVDIGFDSDGIPRIHAANELDAAAALGFVHARDRMFQMDLMRRAASGRLSEIAG
                     AATLAYDRMMRTLGLRQRAVADFASLPADTRAVLEAYARGVNAWIAARGRFSAPEFVV
                     FGPPEQWQASDSLLWGETMGLWLSLNWRTELSRQALASRIPQQQIDELWPPQDGGRPE
                     ASLLPRLPREFADVLPNFPEPFTLPGSASNEWAVDGRHTATGAPLLAGDPHLAFGFPS
                     IWYLARVETPQGVLAGATAPGVPFLVLGHNGHIAWTFTTTGADVQDIFIETPVGADQY
                     QTPDGPRPFTVRDERIKVSGQADEVLKVRETRHGPVISDLTNPGGPILAVQMANLAPG
                     NTAAAGLLTLNHAQTVADAGKAAALITSPVQNLLVADKQQIALYVTGRVPIRRAGDGA
                     APVPGDGSHDWTGWASGDQLPHYVAPGSGRLVNANERIAPTDFPVFMGRDWFGDWRAT
                     RIRTLLERSDKLTLQDFARMQTDVRSDFAHQVLPTLLSVPLSDDTARRAQSLLRDWDG
                     SMTSDAPQPLIFNAWIQYFYGAVLAKAGVGVNDAGPLLEFVGYVLSPAGAHWCGGDCN
                     ALLQSSLADALRELSARFGADPAAWRWGNAHPAVFTHPMLRAVPILNRFATLSIPSPG
                     DDSTVDRGGPAYKKFQSVHGAEYRGVYDLADLDRSLFMMAPGQSGNLLASHARDFLTR
                     WRDGDTITLGPVAATTTATIQLSP"
     gene            complement(2675..3478)
                     /locus_tag="VKQ27_20730"
     CDS             complement(2675..3478)
                     /locus_tag="VKQ27_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HLY91420.1"
                     /translation="MIDSVPPTYEVLALRYGIFDGRLQFQNYIIPDDHAAPDPLDFYV
                     FAIRGNGRTIVMDTGFNPISARRRGRELLRSPADALMQAGIDPTEVPDVVLTHMHWDH
                     AGGMEYFPKATFHLQATEMAYCTGPCMCQPFLRRPFDVDDVQSAIRALYADRLRFHDG
                     TAEIAPGITLHLIGGHSGGIQSIRVPTERGWVVLAGDASHLWGNIRRRMPFPVVVDVA
                     RMLKGFEILESLADGPDHIIPGHDPLILKRFPPVAGYPDTVRVDLPPIA"
BASE COUNT          675 a         1117 c         1358 g          585 t
ORIGIN      
        1 agcgtgaggt aaaacaccag cgtatagatc agcgcctgca gcgccgtcgg cggaccaaga
       61 tcgtcgtcgc gacgcaccgc cagtcccatt gcctgctgcc ccggtgtcgc gccggaaccg
      121 gcgacgaaca ggaaatgata gcagaacggt atgaacggca ggatccccat cagcgggaag
      181 ccgagcccaa gcgtcagcaa gccgaacagc aacaggacga accatagccc cgcgacgatc
      241 aggcagatca gcagcagatc gacgaaccac gcaaagatgc ggcgggtgcg aacgccatcc
      301 gtgagccact cgttgtccgg ataaggcatg cggtagatca tgggctgagc tggattgttg
      361 cggttgtggt tgcggcgacc gggccgagcg tgatcgtgtc accatcgcgc cagcgggtga
      421 ggaaatcgcg cgcgtggctg gccagcaggt tgccggattg tccgggcgcc atcatgaaca
      481 gtgaacgatc gagatcggcg aggtcataga ctcctcgata ctccgctccg tgcacggatt
      541 gaaacttttt gtatgccgga ccgccccgat cgacggtgct gtcgtcaccc gggctcggaa
      601 tgctgagcgt ggcgaaccgg ttaaggatcg gcactgccct cagcatcggg tgggtgaata
      661 ccgcgggatg ggcgttgccc caccgccagg ccgctggatc ggcgccgaag cgtgccgaga
      721 gttcgcgaag cgcgtcggcg agggatgatt gcagcagtgc gttgcaatcg ccaccacacc
      781 agtgcgcgcc ggcaggggat agcacgtagc cgacgaactc cagcagcggg ccggcgtcgt
      841 tcacgccaac cccggctttg gcgaggacag cgccataaaa atactggatc caggcattga
      901 agatcagcgg ctgaggcgcg tcgctggtca tgctcccatc ccagtcgcgc agcagggact
      961 gggctcggcg ggcggtgtcg tcggacagtg gcacggacag cagcgtcggc aggacctggt
     1021 gcgcgaagtc ggagcgcacg tcggtctgca tccgggcgaa atcctgcagg gtgagcttat
     1081 cggagcgctc cagcagggtg cggatgcggg tggcgcgcca atcgccgaac cagtcgcggc
     1141 ccatgaagac aggaaagtca gttggcgcaa tgcgctcgtt ggcattgacc agccggccgc
     1201 ttcccggggc gacatagtgc ggaagctggt cgccggacgc ccaaccggtc cagtcgtggg
     1261 agccatcgcc cgggacaggt gcggcaccgt ctccggcgcg ccggatcggc acgcgcccgg
     1321 tcacgtaaag cgcgatctgc tgcttgtcgg caaccagcag gttctgcact ggcgaggtga
     1381 tgagcgccgc agccttgccg gcatccgcaa cggtctgggc gtggttgagg gtcagcaggc
     1441 cggcggcggc ggtattgccg ggcgcgaggt tggccatctg cacggcgaga atcgggccgc
     1501 caggattggt cagatcgctg atgacaggcc cgtggcgggt ctcgcggacc ttcaggacct
     1561 cgtcggcctg gccactgacc ttgatccgct cgtcgcgcac ggtgaacgga cgggggccgt
     1621 ccggtgtctg atactggtca gcaccgacgg gtgtctcgat gaagatgtcc tgcacgtcgg
     1681 cgcccgtggt ggtgaaggtc catgcgatgt ggccgttatg gcccagcacc aggaacggca
     1741 cgccaggtgc ggtggcgccg gccagcacgc cctgtggggt ctcgacgcgg gcgagatacc
     1801 agatgctggg gaaaccgaag gcgagatgcg ggtcgccggc gagcagtggc gcgccggtcg
     1861 cggtatggcg gccatccacc gcccattcgt tggaagcgct gcccggcagg gtgaacggct
     1921 cggggaaatt gggcagcacg tcggcaaatt cgcgcggcag gcgcgggagc aggctggcct
     1981 ccggacggcc gccgtcctgt ggcggccata gctcgtcgat ctgctgctgc gggatgcggc
     2041 tggcgagcgc ctgacgcgac agctcggtgc gccagttgag tgagagccac aatcccatgg
     2101 tctcacccca cagcaggctg tccgacgctt gccactgctc gggcgggccg aagacgacga
     2161 actccggcgc gctgaaccgg cctcgggcgg cgatccaggc gttcacgccg cgcgcgtagg
     2221 cctcgaggac ggcgcgcgta tcggcgggca ggctagcgaa gtcggcgacc gcgcgctgcc
     2281 gaaggccgag tgtgcgcatc atccgatcgt aggccagggt cgcggcacca gcgatttccg
     2341 atagccggcc cgaggcagcg cggcgcatca ggtccatctg gaacatgcgg tcacgtgcgt
     2401 ggacgaaacc cagggccgcg gcagcgtcca gctcgttggc ggcgtggatg cgggggatgc
     2461 cgtcgctgtc gaagccgata tcgaccgggc cggtcagccc ggggatgcgt gcctgttgct
     2521 ggcgctgcgg caggctaagc cagagcaggc cggtcaccag cgccgccgcg agcaggataa
     2581 ggaagccgac acccaccagg gaacggcgga ggaagcggta gagcgactgc atcccgccaa
     2641 ggtggcggct aggtgcccgg ttgggaagtg aacgtcaggc gatgggcggc agatcgacgc
     2701 gcacggtgtc gggatagccg gcaactggcg ggaatcgctt caggatcagg ggatcgtggc
     2761 cagggatgat gtggtccgga ccgtcggcca ggctttccag gatctcgaag cctttcagca
     2821 tgcgcgccac atccaccacc accgggaacg gcatgcgcct ccggatgttg ccccagagat
     2881 ggctggcgtc gccggccagg accacccagc ctcgctcggt ggggacccgg atcgactgga
     2941 taccgccgga gtggccgcca ataaggtgca gcgtgatacc aggggcgatt tcggcggtgc
     3001 cgtcgtggaa ccgcaggcgg tcggcataga gggcgcggat ggcgctctgc acatcatcaa
     3061 cgtcgaacgg gcgccggagg aacggctggc acatgcaggg gccggtgcag taggccatct
     3121 cggtggcctg caagtggaac gtggccttgg ggaagtactc catgccgccg gcgtggtccc
     3181 agtgcatgtg ggtcagcacg acgtcaggca cctctgtggg atcgatgccg gcctgcatca
     3241 acgcgtcggc gggggagcgc agcagttccc ggcctcggcg cctggcggag atggggttga
     3301 agccggtatc cataacgatg gtgcggccgt tgccgcggat ggcgaagacg tagaagtcga
     3361 gcggatcagg cgcggcatga tcgtccggga tgatgtagtt ctggaattgc aggcggccat
     3421 cgaagatgcc gtaccggagg gcaaggactt cgtaggttgg gggtacactg tcaatcatct
     3481 ggattctccc gatccgctca acctatgccg ccgcaagggg ggctggaaag ttgcaatccg
     3541 gttgactttg ggagtggtga agccacaacg gtgttcgtct tgtccctgtg aggcaggtct
     3601 cgggcaatgc tgtcgtacac ggatcacgct catatccgca tggcgcagcg aggcgtgacc
     3661 gaggctgacg tcgagcgagt cgtcagggat cgcaatttcg tgggcattca ccccgttcac
     3721 ggaactcggc aatat
//
LOCUS       DATGEM010000670         8010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_968545, whole genome shotgun sequence.
ACCESSION   DATGEM010000670 DATGEM010000000
VERSION     DATGEM010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968545"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="VKQ27_20735"
     CDS             complement(<1..1817)
                     /locus_tag="VKQ27_20735"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HLY91421.1"
                     /translation="MENVTARSLLFGTALQTSLAVALALPAHAQPAPNARPTGGVVVG
                     GSASISQTANNTAISQSSQRAAVNWQSFNVGSQQSVNFNQPSASAVTLNRVVGPDPSQ
                     IAGRINANGQVVLVNQSGVTFFKGAQVNTNGLMVSAIGMSNQAVQGFMASGKVVLDQP
                     GNPNAAVINDGNITVRQAGLAALVAPQVANHGTITAELGHVVLAGAKTATLDLYGDGL
                     LSLDVTNQVTQVPVGKDGKPVAALVTNTGVIIADGGTVQLTARAADGIVQNLVDAGGR
                     IRAATVGEQTGTIALNGMGGSIIVEGQLSATGRQPGTTGGAIEVATDQNVVVASTARI
                     NASGKAGGGTVAIGTTLARAKGGPSVTPTLVAANTIIQPGAKISARASGNGNGGRVTI
                     LSTKDTSMAGTVNAQGGPSGGDGGFVEVSSDLGFSLTGAVDVSAPKGADGTILLDPLD
                     LTIGTAGTDNVFLGTIAPQMPFNTIPTDAASGNAFISNTAVESLFGNIDLQASRDLFV
                     NAPITLTAPPTGSGQSLTLEAGRNLVVNAAISTAGNITLTASAPPPTTPPPLTYPAGF
                     STAGALTLNAPVSTSNGGIQLKAGSGGINVNADVSANFVS"
     gene            complement(1968..2708)
                     /locus_tag="VKQ27_20740"
     CDS             complement(1968..2708)
                     /locus_tag="VKQ27_20740"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLY91422.1"
                     /translation="MARLSVNLNKIALLRNSRRTGVPDLAAFARLAVDAGARGITVHP
                     RPDERHIRRSDVPLLAKTIAAQRPHIELNIEGYPDDRLMEICAEVKPEQCTLVPDAPD
                     AFTSEEGWKLDSAQLAEVQPFVTRLKAMGARVILFIDPSPAVLDRIKPAGADGVEIYT
                     GSYAAAWRAGNHAALLDACATTAKAAADRGLIVNAGHDLNLHNLPPLMQRIPFLAEAS
                     IGHELTADALVIGFAGAVTAYAKALGPG"
     gene            complement(2708..3124)
                     /locus_tag="VKQ27_20745"
     CDS             complement(2708..3124)
                     /locus_tag="VKQ27_20745"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLY91423.1"
                     /translation="MPLTVERFQPEGMNVRTSGGKPSYSHAVTVSGTGKLVYTAGQLA
                     RDIDGNCVGKGDMRAQMEQVFQNLDRCLKAAGATWADVVKTNTFVTDFDEFQKCGDIR
                     MRYFGVATPTSTTVGVTRLAGPDFMVEIELVAVVNT"
     gene            complement(3137..4738)
                     /locus_tag="VKQ27_20750"
     CDS             complement(3137..4738)
                     /locus_tag="VKQ27_20750"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY91424.1"
                     /translation="MEGRTFDYIVVGAGSAGSVIANRLSANPQHKVLVLEAGRESHPW
                     SRIPVGFAKLINNPAANWLYSSEPDEGTGNRRIPIPRGKLLGGSSSINGMVFVRGQSQ
                     DYDQWAQLGNRGWSYREVLPIFRDMESYQGEADDEYRGRDGLLKVTESNETGPLYDAL
                     IKAAGSVGIAYTKDYNGANQDGIGMTQATIRNGRRMSTARCYLDPARTRPNLTIQANA
                     LTECLLLEGKRCVGVRYSVNGQTQEARAAREVIVSAGSINSPQLLELSGIGQAERLQG
                     LGIEVRHELKGVGENLRDHYSPRMKWSVPSRLGMTYNDKMRGVGKVKQAMMYALNHKG
                     LLGLPAAPIRAYIRTRQGLEAPDAAISWVPFFMTNDFQLSKQSGITAIMNILRSESTG
                     SIHVTSKSARTPPAVRFNFLTAQLDRDVTLEAMRITRRVMTAPPMKDIATDEVAPGVN
                     IQRDDELLDWVKKNAETTYHPVGTCKMGHDPMAVVDDQLRVHGMQGLRVADASIMPTL
                     TSGNTNAPSIMIGEKASRMILAAAA"
     gene            complement(4799..6400)
                     /locus_tag="VKQ27_20755"
     CDS             complement(4799..6400)
                     /locus_tag="VKQ27_20755"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY91425.1"
                     /translation="MQTYDYIVVGAGSAGAAVANRLSADARNKVLLLEAGRASHPWSR
                     IPIGYARLITNPAANWLYSSEPEASTKGRALPVPRGKLLGGSSAINGMAFVRGQAQDF
                     DTWAQMGNQGWSYEDVLPFFKRMERYEGGDGAFRGHDGPIHVTNPPPRLPLFAALIAA
                     AGQVGIRHNPDYNGATQDGIAMSQASIASGRRMSTARCYLDPIRTRANLRIETGALTE
                     TLLLDGRRCIGVRYSIDSVPHEVRAAREVIVSAGSINSPQLLELSGIGQPERLRGLGI
                     EVRHALPGVGENLRDHFAPRTRWAIGAKGLTYNDTARGLGLVWQALRYALFNDGMLGG
                     VAAPIRAFVRSREGLEAPDVLLGWVPMLTEPGPRGPKISRQSGMTCYAHPMRPESKGH
                     IHITSAEPRRPPAINFNFLSSPIDAEVTARAVGIARSVMTAPAMAHLRVTEIAPGASL
                     NTEDEILDWVRGSAETTYHPVGTCKMGNDPMAVVDAQLRVHGIEGLRVADASIMPTLT
                     SGNTNAPSIMIGEKAADMMLRQAAA"
     gene            complement(6461..7105)
                     /gene="thiE"
                     /locus_tag="VKQ27_20760"
     CDS             complement(6461..7105)
                     /gene="thiE"
                     /locus_tag="VKQ27_20760"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLY91426.1"
                     /translation="MSGRRDGCHIYLITPPKLDPGPFADLLAAALDAGDVAAVQLRLK
                     DVDDDTWRRAIDALRPVTQSREVAFLLNDRADLVRATGCDGAHVGQEDMPAKQARALL
                     GPDATMGVTCKGSRDLAMQAGDDGADYVAFGAFFPSSTKEVTRFIDPEILTWWSELME
                     LPCCAIGGITPENCGPLVRAGADFLAVVGCIWNHPDGPAAGVRTLNKAIADAGA"
     gene            complement(7114..>8010)
                     /locus_tag="VKQ27_20765"
     CDS             complement(7114..>8010)
                     /locus_tag="VKQ27_20765"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HLY91427.1"
                     /translation="VKKILDWYEGDPPGVKANLARILSTGKLGGTGKVLILPVDQGFE
                     HGPARSFAPNPPAYDPHYHYQLAIEAGLNAYAAPLGMIAQGADTFAGQIPTILKVNSS
                     NSLATSKDQAVTAGVKDALRLGCAAIGFTIYPGSEYAFAQMEELRELTAEAKDAGLAV
                     VVWSYPRGPSLDRAGETALDICAYAAQMAALMGANIIKVKPPTAVISLEAAKKSYEKI
                     DGSTLAKRVEHVMQASFNGKRVVVFSGGEAKDLDGVFEEVRGVRDGGGNGSIIGRNTF
                     QRPKEVALEMLNKIIDIYLGKA"
BASE COUNT         1330 a         2554 c         2605 g         1521 t
ORIGIN      
        1 gacacaaagt tggccgaaac gtccgcattc acgttgatgc cgccggaccc cgccttcaac
       61 tggatcccgc cattgctcgt gcttacgggg gcattcagag tcaaagcgcc cgcggtactg
      121 aagccggcag gatatgtgag cggaggggga gtcgtaggag ggggcgccga ggccgtcagc
      181 gttatgttgc ccgccgtgct gatcgcggcg ttgacgacaa gatttctccc cgcttcaagg
      241 gtcaggcttt ggccggaccc ggtgggcggg gcggttagcg tgattggtgc gttgacgaac
      301 aggtctcgcg acgcctggag atcgatattt ccgaacaaac tctcaacggc ggtatttgag
      361 atgaatgcat ttcccgatgc ggcgtcagta ggaatggtgt tgaacggcat ttgaggcgcg
      421 atagtaccta agaaaacatt gtctgttccg gctgtaccta tcgtcaaatc caaaggatcg
      481 agcagtattg tgccgtccgc gcctttgggc gccgaaacat cgacggcgcc cgtaagcgag
      541 aagcccaagt cgctcgatac ttcaacgaaa ccgccatcac cacccgacgg accaccttgg
      601 gcgttgactg tgcccgccat ggatgtgtct ttggtagaca ggatagtgac tcgcccgccg
      661 ttgccattgc cgcttgcacg cgcgctgatc ttcgcaccag gctgaataat ggtgttggca
      721 gcgaccaatg taggcgtgac gctcggcccg cccttggcac gcgccagtgt ggtgccgatg
      781 gcgaccgtgc cgccgcccgc cttgcccgag gcgttgatcc tcgcggtgct cgccacgacc
      841 acgttctggt cggtcgccac ctcgatcgcg ccgcccgtgg ttcccggctg ccggccggtc
      901 gccgacagct ggccctcgac gatgatcgag ccgcccatgc cgttcagcgc gatcgtgccg
      961 gtctgctcgc ccaccgtcgc cgcgcggatc ctgcccccgg cgtccaccag gttctgcacg
     1021 atcccgtcgg ccgcccgcgc ggtgagctgc accgtgccgc catcggcgat gatcaccccg
     1081 gtgttggtca ccagtgcggc caccggcttg ccgtccttgc ccaccggcac ctgcgtcacc
     1141 tggttggtga cgtcgagcga cagcagaccg tcgccgtaga gatccagcgt cgccgtcttc
     1201 gcccccgcca gcaccacgtg accgagttcg gcggtgatcg tgccgtggtt ggccacctgc
     1261 ggcgccacca gtgcggcgag gccagcctgc ctcaccgtga tgttgccgtc gttgatcaca
     1321 gcggcattgg gattgcccgg ctggtcgagc acgaccttgc cgctggccat gaagccctgc
     1381 accgcctggt tggacatgcc gatggcggag accatcaggc cgttggtgtt gacctgggcg
     1441 cctttgaaga aggtgacgcc ggactggttg accagcacga cctgcccgtt ggcattgatg
     1501 cggccggcga tctgcgaggg gtcgggaccg accacgcggt tcagcgtgac ggcggaggca
     1561 gagggctggt tgaaattgac gctctgctgg ctgccgacgt tgaagctctg ccagttgacc
     1621 gcggcacgct ggctggactg gctgatcgcg gtgttattcg cggtctggct gatcgaggcg
     1681 gagccgccga ccaccacgcc accggtgggc cgcgcgttag gcgcgggttg cgcgtgggcc
     1741 ggcagggcga gggcaactgc caggctggtc tgcagagcag taccaaacag caggcttcga
     1801 gctgtgacgt tttccattaa cccggcacgt tacctcaacg aatcgcccat ccatgcgatc
     1861 gtccacacac ttgggtttca catttaattg atcagaggcg cgaacggcac ccgcaggtcg
     1921 attggggcag agaattcatg cgctatgaac ttcaggcgtg cgcaggttca tcctggcccg
     1981 agcgccttgg cgtaggcggt gactgccccg gcgaagccaa tgacaagtgc atcggcggta
     2041 agctcgtgcc caatcgatgc ttcggcgagg aacggaatgc gctgcatgag cggcggcaga
     2101 ttgtgcaggt tgaggtcatg gccggcattc acgatcaggc cgcgatcggc ggccgccttt
     2161 gccgttgtgg cgcaggcgtc cagtagcgcg gcgtggttgc cggcgcgcca ggcggcggcg
     2221 tacgagccgg tgtagatctc cacgccatca gcgccggccg gcttgatgcg atcgagcact
     2281 gctggtgacg gatcgatgaa cagaatgacc cgtgcgccca tcgccttgag gcgcgtgacg
     2341 aatggctgca cctctgcgag ctgtgcagaa tcgagtttcc agccttcctc cgaggtgaag
     2401 gcgtccggcg cgtccggcac cagcgtgcac tgctccggct tcacctcggc acagatctcc
     2461 atcaggcgat cgtcgggata gccctcgata ttcagctcga tatgcgggcg ttgtgccgcg
     2521 atcgtcttgg caagcagcgg gacgtcggag cgacggatat gtcgttcgtc agggcgtgga
     2581 tggaccgtga tgccgcgcgc cccggcatcg acggcaaggc gggcgaacgc ggcaagatcc
     2641 ggcacgccgg tgcgtcgcga attccgcagc agtgcgatct tgttcaggtt gacgctgagc
     2701 cgggccatca ggtgttcacc acggccacca gctcgatctc gaccatgaag tcggggccgg
     2761 ccagacgcgt cacgccaacc gtcgtgctgg tgggcgtcgc cacgccgaaa tagcgcatgc
     2821 ggatatcgcc acatttctgg aattcgtcga agtcggtgac gaaggtgttg gtcttgacca
     2881 catcggccca ggtggcgccg gcggctttca ggcagcggtc gaggttctgg aacacctgct
     2941 ccatctgcgc gcgcatgtcg cccttgccga cgcagttgcc gtcgatgtcg cgcgccagct
     3001 gacccgcggt gtagacgagc ttgccggtgc cgctgacggt cacggcgtgg gaataggacg
     3061 gcttcccgcc agacgtccgg acgttcatgc cttcgggttg gaagcgttca acggtgaggg
     3121 gcatgtcaat ctctccttat gccgctgctg ccagtatcat gcgcgaggcc ttctcgccga
     3181 tcatgatcga cggcgcattg gtgttgcccg acgtcagcgt cggcatgatc gaggcatcgg
     3241 ccacacgcaa gccctgcatc ccatgcacgc gcagttggtc gtcaacgacg gccatcgggt
     3301 cgtgccccat cttgcaggtg ccgacaggat gataggtggt ctcggcgttc ttcttcaccc
     3361 agtcaagcag ttcgtcgtcg cgctggatgt tcacgccagg cgccacctcg tcggttgcga
     3421 tgtccttcat cggcggcgcg gtcatgacgc ggcgtgtgat gcgcatcgcc tccagcgtca
     3481 cgtcacgatc aagctgcgcg gtcagaaaat tgaaccgcac cgcgggcggt gtccgggccg
     3541 acttcgaggt gacgtggatg ctgccagtgc tctccgaccg caggatgttc atgatcgcag
     3601 tgatgccgga ctgcttggaa agctggaagt cgttggtcat gaagaacggc acccaggaga
     3661 tagcggcatc gggcgcctcc agcccctggc gggtacggat atacgcacgt atcggcgctg
     3721 ccggcagccc gagcaacccc ttgtggttca gcgcgtacat catcgcttgc ttcactttgc
     3781 cgacgccgcg catcttgtcg ttgtaagtca tgccgaggcg tgacggcact gaccacttca
     3841 tccgtggcga atagtgatcg cgcagattct cgccaacgcc cttcagttca tgccgcacct
     3901 cgatgcccag cccctgtagc cgttcagcct ggccgatgcc ggacagctcc agtagttgcg
     3961 gcgaattgat cgagccggcg ctgacgatca cctcacgcgc cgcgcgggct tcctgcgtct
     4021 ggccgttgac gctgtagcgc acgccgacgc agcgcttgcc ttccagcagc aggcattcag
     4081 tgagcgcatt ggcctggatc gtcaggttcg ggcgcgtgcg ggcggggtcg agatagcagc
     4141 gagcggtgct catgcggcgg ccattgcgga tggtcgcctg cgtcatgccg atgccgtcct
     4201 gattcgcgcc gttatagtct ttcgtatagg caatgccgac actgccggcc gccttgatca
     4261 gcgcatcgta aagtgggccg gtctcgttgc tttccgtcac cttcagcagc ccatcacggc
     4321 cgcggtactc gtcgtctgcc tcgccctggt agctttccat gtccctgaat atcggcagaa
     4381 cctcgcggta gctccagccg cgattgccga gctgcgccca ctggtcatag tcctgcgact
     4441 ggccgcgcac gaacaccatg ccgttgatcg acgacgaacc gcccagcagc ttgccgcgcg
     4501 ggataggaat ccgacggttg ccggtccctt cgtcaggctc ggaggaatac agccagttcg
     4561 cggcgggatt gttgatcagc ttggcgaagc cgacaggaat ccgcgaccag ggatgactct
     4621 cgcggccggc ttccaggacc agcaccttgt gctgcggatt ggcgctgagg cgattggcga
     4681 tgacagagcc ggccgaaccg gcacctacca cgatgtagtc gaatgttctg ccttccactg
     4741 tttctttccc ccgaaccgtc ccgttcgacg tgcagcgaag caaatcgccc gcggagtctc
     4801 aagccgcagc ctgccgcaac atcatatccg cggctttctc gccgatcatg atcgatggtg
     4861 cgttcgtgtt gcccgatgtc agtgttggca tgatcgatgc gtcggcgaca cgcagacctt
     4921 cgatgccgtg gacgcgcagc tgtgcgtcaa ccacggccat cggatcgttg cccatcttgc
     4981 aggtgccgac ggggtgatag gtggtttccg cgctgccgcg cacccagtcg agaatttcgt
     5041 cctcagtgtt cagcgatgca ccaggggcaa tttccgtcac tcgcagatgg gccattgccg
     5101 gcgctgtcat cacggagcgc gcgatgccga ccgcgcgagc agtgacttcc gcatcgatcg
     5161 gcgaggacag gaagttgaag ttgatagcgg gcggcctgcg cggttccgcc gatgtgatgt
     5221 ggatgtggcc cttgctctcc ggccgcatcg gatgcgcgta gcaggtcatg ccggactgcc
     5281 gcgaaatttt cggcccgcgc gggccgggct cggtgagcat tggcacccag ccgagcagga
     5341 cgtcgggtgc ttccaggcct tcacgtgaac gcacgaaggc gcggatgggg gcagcgacac
     5401 cccccagcat gccgtcgttg aacaacgcgt agcggagcgc ctgccagaca aggccaagtc
     5461 cgcgcgccgt gtcgttgtag gtgaggcctt tcgcgccgat cgcccagcgc gtgcgtgggg
     5521 caaaatggtc tcgcaggttc tcgccgacac cgggcagtgc atggcgcacc tcgatcccga
     5581 gaccgcgtaa gcgttcgggc tggccgatgc cggacagttc caacagttgc ggcgagttga
     5641 tgctgccggc gctgacgatg acttcgcgcg cggcgcggac ttcgtgtggc acgctgtcga
     5701 tagagtagcg gactccgatg cagcgcctgc cgtcgagcag cagtgtttcg gtgagcgcac
     5761 cggtctcgat gcgcagattg gcgcgtgtgc ggatcgggtc gagatagcac cttgccgtac
     5821 tcatgcggcg gcctgatgca atggacgcct ggctcatcgc gatgccgtcc tgggtggcac
     5881 cgttgtagtc cgggttgtgc cggatgccga cctggccggc cgcggcaatc aaggcggcga
     5941 acagcggcag gcgtggcggc gggttggtga catggatcgg cccgtcgtgg cctcggaagg
     6001 cgccgtcgcc gccttcgtag cgctccatgc gcttgaagaa gggcaggacg tcctcgtagc
     6061 tccagccctg gttgcccatc tgcgcccagg tatcgaagtc ctgcgcctgg ccgcgcacga
     6121 aggccatgcc gttgatggcg ctggacccgc ccagcagctt gccgcgcggg acgggaagtg
     6181 cgcggccctt cgtgctggct tcgggctccg agctgtacag ccagttcgca gccgggttgg
     6241 ttatcaggcg ggcatagccg atggggatgc gcgaccaggg atggctggcg cgaccggctt
     6301 ccagcaacag caccttgttg cgtgcatcgg cggagaggcg gttggcgacg gcagcaccgg
     6361 cggaacccgc gccgacaacg atgtagtcat aggtctgcat aatgcgcgga ttgcgctaac
     6421 cggaacgatt gtcaatgcgg cgggcggccg gtcatggaag tcaggctccg gcgtcggcaa
     6481 tcgccttgtt cagggtgcgc acgccggctg ccgggccatc ggggtggttc cagatgcagc
     6541 caactaccgc gaggaaatca gcgccagccc ggaccagtgg gccacagttc tcaggcgtga
     6601 tgccaccgat ggcgcagcag ggcagctcca tcagttccga ccaccacgtc aggatctcgg
     6661 gatcgatgaa gcgcgtcact tccttggtgc tcgacgggaa gaacgcgccg aacgcgacgt
     6721 agtcggctcc atcgtcgccc gcctgcatcg ccagatcgcg gctgcccttg caggtgactc
     6781 ccattgtcgc atccgggccc aacaacgcac gcgcctgctt cgccggcatg tcttcctgcc
     6841 cgacatgcgc gccgtcgcag cctgtggcgc gaaccagatc ggcccggtcg ttcagcagga
     6901 acgccacctc gcgcgactgc gttaccggtc gcagcgcatc gatcgcgcgg cgccatgtgt
     6961 catcatcaac atctttcagg cggagctgca cagccgccac atcaccggca tccagtgccg
     7021 ctgccagaag atcggcgaac ggccccggat ccagcttcgg cggggtaatg agatagatgt
     7081 ggcaaccgtc ccggcggccg ctcatgtcgg gcatcaggcc ttgccgaggt agatatcgat
     7141 gatcttgttc aacatctcca gcgcgacctc cttcgggcgc tggaaggtgt tgcggccgat
     7201 gatgctgccg ttgccgccgc catcgcgaac gccgcgcact tcttcgaaca cgccgtcgag
     7261 atccttcgcc tcgccgcccg agaacaccac aacgcgcttg ccattgaacg aggcctgcat
     7321 cacgtgctcg acccgcttcg cgagggtcga gccatcgatc ttctcgtacg acttcttcgc
     7381 ggcctcaagg ctgatcacgg ctgtcggcgg cttcaccttg atgatgttgg ctcccatcag
     7441 cgctgccatc tgcgcggcat aggcgcagat atcgagcgcc gtctcgccag cccgatccag
     7501 gctcggacca cgcggatagc tccacaccac gactgcgagg ccggcgtcct ttgcttcggc
     7561 tgtcagttcg cgcagctctt ccatttgcgc gaaagcgtat tcgctgccgg gatagatggt
     7621 gaacccgatg gccgcgcagc caagacgcag cgcgtccttc acacccgccg tcaccgcctg
     7681 gtccttgctg gtggcaagcg aattcgagct gttgaccttg aggatcgtgg gaatctggcc
     7741 ggcgaatgtg tccgcaccct gcgcaatcat ccccagcggg gcggcgtagg cgttcagccc
     7801 ggcctcgatc gcaagctgat aatggtagtg cggatcatag gctggtggat ttggtgcaaa
     7861 gctgcgggcc ggcccgtgct caaaaccctg atccaccggc aggatcaaca ccttgccggt
     7921 accgccaagc ttcccggtgg agaggatgcg cgcgaggttc gccttcacgc caggcgggtc
     7981 gccctcatac cagtccagaa tcttcttcac
//
LOCUS       DATGEM010000671         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_734744, whole genome shotgun sequence.
ACCESSION   DATGEM010000671 DATGEM010000000
VERSION     DATGEM010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734744"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VKQ27_20770"
     CDS             <1..1281
                     /locus_tag="VKQ27_20770"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HLY91428.1"
                     /translation="LAFALRRAGVGNVTILEAAPDEQRAGLWLNAARMNLLRTPKTLP
                     GPELGIPALSFQAWYEARHGQAAYAAIDRIPRTDWADYLSWYKTFLGISPRYRTRLER
                     IEPTGDRLTLHLDTPDGSATETASKVILATGFAGSGGNYIPDVLVNNLPRERYAHTED
                     VIDFAALRGKVVGVIGAAASAFDAAGVALEHGAAEVHLFARRDAIAAVPITRSRGFPG
                     AYDNYFLLPDADRWHQAFRFFAYGSTPTTDAIERAVRFGNFHLHLSAPWSNAAMQDGQ
                     VVATVGGTRHPLDFVIAGTGYSADLASRPELRDFAGQILLWRDVYTPAPAEQDERLGC
                     APYLGAGHELRAKTPQASPLLKDIRVQNPAGFVSFGLPIGDVPSMKRDIPVIVSRISA
                     DLFLENLGVLRERMTGSVPTDFSDALYRAAVRQP"
     gene            complement(1297..1650)
                     /locus_tag="VKQ27_20775"
     CDS             complement(1297..1650)
                     /locus_tag="VKQ27_20775"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HLY91429.1"
                     /translation="MQDIAAIKDYHAHIYYDASTKPQAAQLRQWVEDRFAGQMRMGSW
                     HDEEVGPHVQAMYQIAFPPELFPTLVPFIMMNRMGLTVLVHPQSGRPRDDHTVNAMWM
                     GEVLPVKTDVLREVG"
     gene            complement(1650..3179)
                     /locus_tag="VKQ27_20780"
     CDS             complement(1650..3179)
                     /locus_tag="VKQ27_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HLY91430.1"
                     /translation="MIPTSNLAHLLLQTARRFPERPAIIWREHTWTWTEFASRVQRAA
                     GALAERGVQHGDRVLLHARNSNVVLETMFASWMLGATWVPTNFRLTPPEVAYLARSSA
                     AAVHIFDDAFPDHAAAARAENPNVRLHISIGEWDTLAISDHALPRPADVDRDHPAWFF
                     YTSGTTGRPKAGVLTHGQMEFVICNHLCDLMPGTTESDVSLVVAPLSHGAGIHALAQI
                     ARGAASVLLSGERLDCEEAWQLVERHRVSNLFTVPTILTMLTRHDAVDRHDHSSLRYV
                     IYAGAPMYRADQVHALRKLGQVLVQYFGLGEVTGNITVLPPALHSIDDDMKAGSCGFP
                     RTGMDITILDVDGAHLPPGTTGEICVRGPGVFAGYYNNPEATAQATRFGWFHTGDLGH
                     MDEQGFVYITGRASDMYISGGSNVYPREIEELLLTHPAVVEACVVGMPHERWGEAGVA
                     VLVLADGAQPTEPELLAHLEGRLAKYKWPARFAFWPELPKSGYGKVAKRDVKRLLEET
                     A"
     gene            complement(3176..>3535)
                     /locus_tag="VKQ27_20785"
     CDS             complement(3176..>3535)
                     /locus_tag="VKQ27_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495491.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HLY91431.1"
                     /translation="DMADVKLDELSFVESHDCFTIAELLEYEAMGLTATGQGARAVLE
                     GWTAKDGRLPVNPSGGLKAKGHPIGATGVSMHAITAMQLTGLAGTMQLPKADIAGVFN
                     MGGAAVANYVSILEAVR"
BASE COUNT          605 a         1140 c         1148 g          642 t
ORIGIN      
        1 ctggccttcg cattgcgccg cgccggagtt ggcaatgtca ccatcctgga agcggcgccg
       61 gacgaacagc gcgcaggcct ctggctgaac gccgcgcgga tgaacctgct gcgtacgccg
      121 aagacgctgc cggggccgga actgggcatt cccgcgttga gcttccaggc atggtacgag
      181 gcgcgccatg gccaggcggc atatgccgcc atcgatcgca ttccgcgcac cgactgggcg
      241 gattacctga gctggtacaa aacgtttctt ggcatcagcc cacgctaccg aacacggctg
      301 gagcggatcg agccgaccgg tgatcgtctg acgctgcatc tcgatacgcc cgacggcagt
      361 gcgacggaga cggcgagcaa ggtcatcctg gccaccgggt tcgccggcag cggcggcaac
      421 tacattccgg atgtgctggt caacaatctg ccgcgtgaac gctatgccca caccgaggat
      481 gtgatcgatt tcgccgcgct gcgcggcaag gttgtcggtg tcatcggtgc ggcggcatcc
      541 gctttcgatg ctgccggcgt cgcgctggaa catggtgcag cggaggtcca tttattcgca
      601 cgacgggatg cgatcgcggc cgtgccgatc acccgatcgc gcggcttccc aggcgcctat
      661 gacaattact tcctgttgcc ggacgcggat cgctggcacc aggcgttccg tttcttcgct
      721 tatggctcga cacccacgac cgatgcgatc gaacgcgcgg tgcggttcgg caacttccac
      781 ctgcatcttt cggcgccctg gagcaacgcg gcgatgcagg acgggcaggt ggtggcgacg
      841 gttggcggca cgcgccatcc gctggacttc gtgattgccg gcacggggta ttccgccgat
      901 ctggcgtcgc ggccggaact gcgcgacttc gccggccaga tcctgctgtg gcgcgacgtc
      961 tatacgccgg caccggcgga acaggacgaa cggctgggct gtgcgcctta ccttggcgcc
     1021 ggacacgagc tgcgggcgaa gacgccgcag gcgtcgccac tgctgaagga catccgcgtg
     1081 cagaaccctg caggcttcgt cagcttcggc ttgccgatcg gcgacgtccc cagcatgaag
     1141 cgcgacattc cggtgatcgt gagccgcatc agcgccgatc tgttcctgga gaatctgggc
     1201 gtgttgcgcg aacggatgac aggcagcgtg cctacggact tttccgatgc gctctaccgg
     1261 gcggcggtgc ggcagccgta gcgcccggag gcccggctat ccgacctcgc gcagcacgtc
     1321 ggtcttcacc ggcagtactt cgcccatcca catcgcattc accgtgtggt cgtcgcgcgg
     1381 ccgcccggat tgcggatgca ccagcaccgt cagtcccatg cggttcatca tgatgaaggg
     1441 taccagcgtc ggaaacagtt ccggcggaaa ggcgatctgg tacatcgcct gtacgtgcgg
     1501 gccgacttcc tcatcgtgcc agcttcccat acgcatctgg ccggcgaaac gatcctctac
     1561 ccattgccgc aattgcgctg cctgcggctt ggtggacgca tcataataga tgtgcgcgtg
     1621 gtagtccttg atcgcggcta tgtcctgcat cacgcagtct cctccaagag gcgcttcacg
     1681 tcgcgcttgg caaccttgcc gtaacccgat ttgggcagtt ccggccagaa tgcaaatcgc
     1741 gccggccatt tgtacttcgc aagcctgcct tcgagatggg ccagcaattc gggttcggtc
     1801 ggctgggcgc cgtctgccag gaccagcacg gcgacgccgg cttcgcccca gcgctcgtgc
     1861 ggcatgccga cgacgcaggc ttccaccacg gcgggatggg tcagcagcag ctcttcgatt
     1921 tcgcgtggat atacgttgga gccgccggag atatacatgt cggacgcgcg gccggtgata
     1981 tagacaaacc cctgttcgtc catgtggccc aggtcgccgg tatggaacca gccgaagcgt
     2041 gttgcctgcg cggtggcttc ggggttgttg tagtagccgg cgaatacgcc tggacctcgg
     2101 acgcagatct cgccggtggt tcctggcggg agatgcgcgc catcgacgtc gaggatggtg
     2161 atgtccatac ccgtgcgggg aaaaccgcag cttccggcct tcatgtcatc gtcgatgctg
     2221 tgcagggcag ggggcagcac ggtgatgttg ccggtcacct cgccgaggcc gaaatactgc
     2281 accagcacct ggccgagctt gcgcagcgca tgcacctgat ccgcacgata cattggcgct
     2341 ccggcgtaga tcacatagcg cagcgatgaa tggtcgtggc gatccactgc atcatgtcgt
     2401 gtcagcatgg tcaggatggt cggcacggtg aacaggttgc tgacgcgatg ccgctcgacg
     2461 agctgccagg cttcctcgca gtccaggcgt tcaccggaca gcaacacgct ggcggcgcct
     2521 cgggcgatct gcgccaacgc atggatgccg gcgccatgtg acagcggcgc cacaaccagc
     2581 gagacatcgc tttcggtggt gccgggcatc aggtcgcaca gatggttgca gatcacgaat
     2641 tccatctgtc cgtgcgtcaa cacgcctgcc ttcgggcgcc ccgtagtgcc agacgtatag
     2701 aagaaccacg ccggatgatc gcggtcgaca tcggcaggtc gcggcaacgc gtggtccgat
     2761 atcgcgagtg tgtcccactc gccgatggaa atatgcaggc gcacattggg attttcagca
     2821 cgggcggcgg ccgcgtggtc ggggaacgcg tcgtcgaaga tatgcacggc ggcggcggat
     2881 gatcgcgcca gatacgccac ctcgggcggc gtgaggcgga agttcgtcgg cacccacgtc
     2941 gcgccgagca tccatgacgc gaacatcgtc tccagcacga cgttggaatt gcgggcatgc
     3001 agcagcacgc ggtcgccatg ctgcacgccg cgctcggcca aggcaccggc ggcacgctgt
     3061 actcgcgatg cgaactctgt ccaggtccag gtgtgttccc gccagatgat cgccggccgt
     3121 tccgggaacc gccgcgcggt ctgcaacagc agatgcgcga ggttgctggt tgggatcagc
     3181 gcaccgcctc caggatcgac acgtagttgg caactgccgc cccgcccatg ttgaacacgc
     3241 cggcgatgtc cgccttcggc aactgcatgg tgcccgccag gccggtcagc tgcatcgcgg
     3301 tgatcgcgtg catcgagaca ccggtcgcac cgatcggatg gcccttcgcc ttcaatccac
     3361 cagaaggatt gaccggcagc ctgccgtcct tggcagtcca gccttccagc acggcgcgag
     3421 cgccctggcc cgtcgcggtc aggcccatgg cctcgtattc cagcagctcg gcgatggtga
     3481 agcagtcgtg cgattcaacg aaggacagtt cgtccagctt cacgtccgcc atgtc
//
LOCUS       DATGEM010000672         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_848549, whole genome shotgun sequence.
ACCESSION   DATGEM010000672 DATGEM010000000
VERSION     DATGEM010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848549"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..2193)
                     /locus_tag="VKQ27_20790"
     CDS             complement(169..2193)
                     /locus_tag="VKQ27_20790"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLY91432.1"
                     /translation="MRTDEGESATTLDRVLLDHGLTVGRQAMPGQVVLDHPNVSRRHA
                     AFEVVDGTIVLRDLGGTNGTYVNGTRLRGARPLVPGDRIDIGPFELTFDGIALTQAWR
                     VGNVELLVHDVSFDVPGRRSGAAPQRILHAANLRIRPSEFVAIIGANGSGKSTLMNIM
                     AGRAFPSEGAVLLNGGDLHGNFAALKQDIAFVPQQDILHELLTLRQALDYAAQLRLPP
                     DTTFEQRRAAVNDAARNVDLLDRLDQRIGSLSGGQKKCASLASEILNRPSLLLLDEVT
                     SGLDESTDWEIMRLLRRLADDGMTIVMVTHTLANVTEFCDKIVCMGRGGQPTFVGAPS
                     EVMDFFAVRRLGEVFSRTDEVGAENWRDRFEATVGASANWPTAAEARGELPRPDIQRP
                     REPVLVTLRRVERQAAVLLHRNARLLLADRRTLIMAAVQSVLIGGLIGYAFGSFGTGL
                     EQVNAQNALLLLLGLSAIWLGCNAASKDIVGELTIYRREHDINLSTAAFVGAKYIVSC
                     VFTLLQLAAVYVLAAALSDGIPGDRLEQFLLLTIGAMAGTAMGLVISAFANTRDQATM
                     IVPLALVPQLILAGVLVPKLPGPAIAVAKVAVSGYWLTEAMKSVFIAADGPIRVINVH
                     TGRLMDMTAEPAVRGAAIVAAQVLVFLLIAYVVTLLRHGGRNPGARGCVS"
     gene            2430..>2646
                     /locus_tag="VKQ27_20795"
     CDS             2430..>2646
                     /locus_tag="VKQ27_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91433.1"
                     /translation="MREGLVGIVPGYGKRQPCQCRNRLRTGFLHDRGAMILDGALADA
                     KVRGDILAGVSRKDERHDLMLTWRELRD"
BASE COUNT          513 a          848 c          822 g          463 t
ORIGIN      
        1 gatcggtcat ccgaaccgag ggcatggccc gccgcagcaa cgcaaaatcg gattgaaaaa
       61 gctcgaatga cgatcggttg catttgccga ataacgccaa ggaccgtgcc gcggtgcacg
      121 gtcacacaat gggcaagaac cttgacgtga gataggttcg gaaactactc aggagacgca
      181 gcctcgggcg ccaggattcc gtcccccgtg gcgcagcagc gtcaccacgt acgcgatcag
      241 caaaaacacc agaacctgcg cggcgacgat cgcagcacca cggactgccg gctccgcggt
      301 catgtccatc agcctgccgg tgtgtacgtt gatcacccgg atcggtccat ctgcggcaat
      361 aaataccgac ttcatcgcct cggtcagcca gtagccgctg actgctacct tcgccacggc
      421 gatggccggg cccggtagct tcggcaccag caccccagcc agaatcaatt gcggaaccag
      481 ggctagtggg acgatcatgg ttgcctggtc tctcgtgttg gcgaacgccg agatcaccag
      541 gcccatcgcg gtgcccgcca tcgcaccgat cgtcagcagc aggaattgct ccagccgatc
      601 accggggatg ccgtccgaca aagctgccgc aagcacatag acagccgcga gctgcagcag
      661 agtgaacacg catgagacga tgtatttcgc gccgacgaac gccgcagtgg acaggttgat
      721 gtcgtgctcg cgtcgataga ttgtgagttc accgacgatg tccttcgatg ccgcgttgca
      781 tccgagccag atcgcgctga gaccgagcag aagcagaagc gcattctgcg cgttcacctg
      841 ctccagcccg gtcccaaaac tgccgaacgc gtagcctatc agtccaccga tcagcacgct
      901 ctgtacggcc gccatgatca gcgtgcgacg atcggccagc aacaggcgcg cgttccgatg
      961 cagcagaacg gccgcttggc gctcgacgcg tcgcagcgtg acaaggacgg gctcacgagg
     1021 gcgctggatg tcgggacgcg gcagttcgcc gcgcgcttcg gcagcggtcg gccagttcgc
     1081 cgacgcgccg acggttgcct cgaacctgtc tcgccagttc tcggcgccaa cctcatccgt
     1141 gcggctgaac acctcgccca atcgacgcac ggcgaagaaa tccatcacct cggagggcgc
     1201 cccaacgaat gtgggctggc cgccgcggcc catacagaca atcttgtcac agaactccgt
     1261 cacgttcgcc aaggtgtggg ttaccatgac gatggtcatc ccatcgtcgg ccaggcggcg
     1321 gagcaggcgc atgatctccc agtcggtgct ctcgtccagc ccactggtca cctcgtctag
     1381 cagcagcagg cttggccggt tgagtatctc gctggccagg ctcgcgcatt tcttctggcc
     1441 gccgctgagt gaaccgatgc gctggtcgag ccgatccagc aggtcgacat tccgcgccgc
     1501 atcgttcacg gccgcgcggc gctgttcgaa ggtggtatcg ggtggcaggc gaagctgtgc
     1561 cgcgtagtcg agtgcctgac gcagcgtaag tagctcgtgc aggatgtcct gctgcggcac
     1621 gaatgcgata tcctgcttca atgccgcaaa attgccgtgc aggtcgccac cgttgagcag
     1681 taccgccccc tcgctcggga aagccctgcc agccatgatg ttcatcaacg tggacttgcc
     1741 cgagccgttg gcaccgatga tggcgacgaa ctcggacggg cgaatacgca ggttggcggc
     1801 gtgcaggata cgctgtggtg ccgcacccga ccgcctgccc ggcacgtcga agctgacgtc
     1861 gtgcaccagc aactcgacat tgccgacgcg ccaagcctgg gtaagcgcaa tgccatcgaa
     1921 ggtcagttca aacgggccga tatcgatgcg gtcacctggg accaaaggac gggcgccacg
     1981 caaccgcgta ccgttgacat aggtaccatt ggtgccgccg aggtcgcgca gcacgatggt
     2041 tccgtccacc acctcgaacg ccgcgtgacg gcgcgacaca ttcggatggt cgagcacgac
     2101 ctgccctggc atcgcttgac gcccgacggt caggccgtgg tcgagcagta cacggtcgag
     2161 tgtggtggca ctctcaccct catctgttcg cacgatccgc ggccaatttg gggtgggcgt
     2221 gggggccgtc ggccgctcac ccagagatcg ctgactgctt cccatcatgt cggccgcttc
     2281 ctcaagcagc caaccggacg tccttcccga atggaacccg accgtatgcg gttggccgct
     2341 gcgtcggcag gataccgacc aatcgcgtgg cgcgggaggt tcggaaacta cctagtgact
     2401 ggcgacatgg ccgtggacgg agatctgcgt tgcgcgaagg gctcgtcggt atcgttccag
     2461 gttacggcaa gcgccagccg tgccaatgcc ggaacagact tcgaaccggt tttcttcatg
     2521 atcgtggcgc gatgattctc gacggtgcgt tggctgatgc caaggtccgc ggcgatattc
     2581 ttgctggggt gtcccgcaag gacgagcgcc atgatctgat gctgacgtgg cgtgagctca
     2641 gagaca
//
LOCUS       DATGEM010000673         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1047822, whole genome shotgun sequence.
ACCESSION   DATGEM010000673 DATGEM010000000
VERSION     DATGEM010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047822"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..713
                     /gene="folP"
                     /locus_tag="VKQ27_20800"
     CDS             12..713
                     /gene="folP"
                     /locus_tag="VKQ27_20800"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLY91434.1"
                     /translation="MVDVGGESTRPSSHQTSPEVEQARIVPVIRALAAAGVHVSVDTR
                     HAATMAAALDAGAVIVNDVYALGFDPAAAALVAERGCAVVLMHMRGTPADMYAQAHYT
                     DIAGEVARELGRRISMAEEAGLSRDKIAVDPGIGFAKTAEQSVDILRRLEELEALGLP
                     ILVGVSRKSFIGAVTGEREPKRRLPGSLAAGLAALSRGASILRVHDVVESIQAIKVWR
                     ALDMLPLGGAQRLDD"
     gene            731..2101
                     /gene="glmM"
                     /locus_tag="VKQ27_20805"
     CDS             731..2101
                     /gene="glmM"
                     /locus_tag="VKQ27_20805"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLY91435.1"
                     /translation="MARVRHLFGTDGIRGTANCDPMTAEIALRLGQAAGLLFTRGSHR
                     HRVVIGKDTRLSGYMIEPALTAGFIGAGMDVTLVGPLPTPAIAMLTRSLRADLGVMIS
                     ASHNPFEDNGIKLFGPDGFKLDDETEAEIEELMASDMRHRMAAPGLLGRAARLEDAAG
                     RYIEAAKASFPRGLRLDDLKIVVDCANGAAYRVAPTVLWELGATVVPVGVSPDGFNIN
                     RDCGSTVPEHLCAQVREHGAQLGIALDGDADRLLIADETGELIDGDQILALIAQSWAA
                     NGRLRGEGIVATVMSNLGLERFLAAKGIDLHRTKVGDRYVAARMREGGMNLGGEQSGH
                     VILSDFATTGDGLLAALQVLAVIVEQGRSVRDVCRMFTPMPQLLKNVRFAGRSPLRSR
                     PIQQAIADAEASLIGSGRLVIRESGTEPLIRVMAEADDEVLVGRIVDELCATIAAATR
                     VSESAD"
     gene            2101..2892
                     /gene="thiD"
                     /locus_tag="VKQ27_20810"
     CDS             2101..2892
                     /gene="thiD"
                     /locus_tag="VKQ27_20810"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HLY91436.1"
                     /translation="MRGRVLVIAGSDSGGGAGIQADIKTITMFGAYAATAITALTAQN
                     TLGVEAVHVAPLDFVTKQIEVVMRDLGADVVKTGMLADAATIEAVCEALARFAPGVPV
                     VVDPVMVATSGARLLADDALSMLMRRLLPMACVITPNLPEAEALAGMPIPDVAAMHRA
                     ADALLSLGVPAVLLKGGHLPGTQVVDLLATEDGAEEFAAPRIDSRNTHGTGCTLASGI
                     AASLAQGMTLRDAVLRARDYVRAAIASAPGFGAGHGPLNHAVGIG"
     gene            complement(2871..3506)
                     /locus_tag="VKQ27_20815"
     CDS             complement(2871..3506)
                     /locus_tag="VKQ27_20815"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLY91437.1"
                     /translation="MPRTRQTDDGPVTLRRRILRAAFEEFMQRGYAGTSTLDIASRAK
                     VSKRDLYAEFGSKHAMLEACIAERAERMRLPLQLPAPRDRDGLLATLMTFGASLLSEA
                     ARPEVIAVYRLAIGEAERSPDIARTLDTLGRAQTRQALSRLFVHSQSLGLIGSGNAGD
                     MAEDFIALLWRGNLLMRLQLGTVAVPGAKECERRAENAAHSLLKLYPIPTA"
     gene            3586..4290
                     /locus_tag="VKQ27_20820"
     CDS             3586..4290
                     /locus_tag="VKQ27_20820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91438.1"
                     /translation="MSNAAPVARLDHVGKTYHAGKIDVAAIRDISMAIPTQRFSMIIG
                     PSGSGKTTLLNLIGCIDTPTAGRIEVCGEDIGRLSDNALADFRARNIGFIFQGFSLVP
                     VLSAYENVEYPLMLVGMATGERRRRTVAMLDAVGLSEQANQRPNELSGGQKQRVAIAR
                     ALVKQPNLVLADEPTANLDTATGAAIIALMRRMQAEQNTTFIFSTHDPQLISHSEETF
                     TIRDGVLMSHATERSD"
     gene            4290..>5606
                     /locus_tag="VKQ27_20825"
     CDS             4290..>5606
                     /locus_tag="VKQ27_20825"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLY91439.1"
                     /translation="MLFRIAFRNILRNRRRSLMTGSAVAVGTLAMLLFGCFIGYIFAA
                     LETGLVQRVGHLSVFRSGYFVFGAGNPSAYGIDDYRGVMRMIGGDPVLKPMVRVLTPR
                     QSLVGIAGNFSGGNDAAKTFLGIGLVPSDRSTMREWNEHGTAPLYVPDDRMSDDAPSR
                     GLIGGGMARILGLCAPLKLADCPAMPKHDEPSSEHAPAAQDLSSLARAELGSQGPAKT
                     TPQLDLLSATAAGAPNVVSMDVAGVDLQSAKELDDAYVAMPLPLAQQLVYGRGEHKVT
                     GIVLQLQRTEDLAAARARLETLIGEHHLDLEVHDFAELTPFYVQVVRLFGTIFLFIAL
                     IMAIIVLFAVVNTMTMNVVERTNEIGTIRAMGVRRNGIRAQFVLEGWLLGAIFAAVGV
                     VLAFVVATAINHAGLTWSPPGNANAVPFRLAIPSSRPLLLAGTWIGL"
BASE COUNT          920 a         1637 c         2025 g         1024 t
ORIGIN      
        1 ccggcgcgga cattgtcgat gtgggtggcg agagcacgcg gccatcgtcg caccagacgt
       61 ctccggaggt tgaacaggcg cgtatcgtac ctgtgatccg ggcgctggct gccgctggtg
      121 tccatgtgtc tgtcgataca cgccatgctg ccacgatggc tgcggcgctg gatgccggag
      181 ccgtcatcgt gaacgacgtc tatgcactgg gattcgatcc ggctgcggcg gcgctggtgg
      241 cagagcgggg atgtgcggtg gtgcttatgc atatgcgcgg cacgccggcc gacatgtatg
      301 cacaagccca ttacacggat atcgcgggcg aggtggcgcg ggagctcggg cgccgcattt
      361 cgatggcaga ggaggcgggg ctgtcccggg acaaaatcgc cgtcgatccg gggatcggtt
      421 ttgccaagac cgcggaacag tcagttgata ttcttcgtcg gctggaggag ttggaggcgc
      481 tcgggttgcc aatccttgtc ggcgtttcgc gcaagtcgtt catcggcgcg gtgaccggcg
      541 agcgggagcc gaagcggcgg ctgcccgggt cgctggcggc ggggcttgct gctctgtccc
      601 gcggcgcgtc aatcctgcgt gtacacgatg ttgttgaatc gattcaggcg ataaaggtct
      661 ggcgggcgct ggatatgctt cctttgggtg gtgcgcagcg actagatgat tagtgggaga
      721 cggagcctcg atggcgcgcg tacgacacct gttcggcact gacggcattc gtggaaccgc
      781 caactgcgat ccgatgacgg cggagatcgc gctgcgcctc ggccaggcgg ccgggcttct
      841 gttcacgcgc ggcagccacc ggcaccgcgt ggtcatcggc aaggacacgc ggctgtcggg
      901 ttacatgatc gagcccgcgc tgaccgccgg ctttatcggt gccgggatgg atgtcacgct
      961 ggtcgggccg ttgccgacgc cggcgatcgc catgctgacc cgcagcctgc gcgcagatct
     1021 tggggtgatg atctcggcct cgcacaatcc gttcgaggac aatgggatca agctgttcgg
     1081 tccggacggt ttcaagctgg acgacgagac cgaagccgag atcgaagaac tgatggccag
     1141 cgacatgcgc caccggatgg cggcgccggg gctgctcggg cgcgccgctc gcctggaaga
     1201 tgctgcgggc cgttatatcg aggcggccaa ggccagcttc ccacgcggtc tgcgcctgga
     1261 tgacctgaag atcgtggtgg actgcgctaa tggcgctgcc taccgcgtgg cgccgacggt
     1321 gctgtgggaa cttggcgcca ccgtggtgcc ggtcggcgtg tcaccggacg gattcaacat
     1381 caaccgtgac tgtggctcga ccgtgccgga gcatctatgc gcccaggtcc gggagcacgg
     1441 ggcacagctt ggcattgcgc tggatggcga tgccgatcgg ctgctgatcg cggacgagac
     1501 gggcgaactg atcgatggcg accagatcct ggccctgatc gcgcagtcct gggcagcgaa
     1561 cgggcggctg cgcggcgagg gtatcgtcgc cacggtgatg tccaacctgg ggctggagcg
     1621 cttcctggcg gcgaagggga tcgatctgca ccgtaccaag gtgggcgatc gctatgtcgc
     1681 ggcccgcatg cgtgaagggg gaatgaacct ggggggcgag cagtcaggtc acgtgatcct
     1741 gtccgacttc gccacaacgg gcgatggtct gcttgccgca ttgcaggtgc tggcggtgat
     1801 cgtcgagcag ggacgttcgg tgcgcgatgt gtgccggatg ttcacgccga tgccgcagct
     1861 gctgaagaac gtgcgctttg ccgggaggtc gccgctccgc agcaggccga tccagcaggc
     1921 gatcgccgac gcggaggcgt cgttgatcgg ttccgggcgg ctggtgatcc gggaaagcgg
     1981 taccgagccg ctgatccggg tcatggcgga agcggacgat gaggtgctgg tcgggcgcat
     2041 tgtcgatgag ctgtgcgcga cgatcgcggc cgccacgcgt gtgagcgaaa gcgccgactg
     2101 atgcggggcc gcgtgctggt gatcgctggc tcggattcgg gcggcggggc cggcatccag
     2161 gccgatatca agacgatcac gatgttcggt gcctatgccg cgacggcgat caccgcgctg
     2221 actgcacaga acacgctggg agtcgaggcg gtgcatgtgg cgccgctgga tttcgtcacc
     2281 aagcagatcg aagtggtgat gcgcgacctc ggtgccgacg tcgtgaagac cggcatgctg
     2341 gcggatgccg cgacgataga ggcggtgtgc gaggcgctcg cccggtttgc gccaggagtg
     2401 ccggtggtgg tcgatccggt catggtggcg accagtggcg cacggctgct ggcggatgat
     2461 gcgctgagca tgctgatgcg gcgcctcttg ccgatggcct gcgtgatcac gccgaatctg
     2521 cctgaggcgg aggcgctggc cggcatgccg atcccagatg tcgcagcgat gcatcgggcg
     2581 gccgacgcgt tgctgtcgct aggcgtgccg gcggtgctgc tgaagggcgg gcatctgccc
     2641 ggtacgcagg tggtggacct gctggcgacc gaggatggcg cggaagagtt cgctgcgccg
     2701 cggatcgact cacgcaatac gcatggcacg ggatgcacgc ttgctagcgg catcgcggcg
     2761 agcctggcgc aggggatgac actgcgcgac gcggtcctgc gggcacggga ctatgtccgc
     2821 gcggcgattg cctcggctcc tggcttcggc gccgggcacg ggccgctcaa tcatgcggtg
     2881 gggatcgggt agagcttcag gagtgagtgg gcggcattct cggcacgccg ttcacactcc
     2941 ttggcgccgg gaacagcgac ggtgccgagt tgcagccgca tcagcaggtt cccgcgccac
     3001 agcagcgcaa tgaagtcctc ggccatgtcg ccggcgttgc cggagccgat cagtccgaga
     3061 gactgactgt gcacgaacag ccgcgacagc gcctgccgcg tttgcgcgcg ccctaacgtg
     3121 tcgagcgtgc gagcgatatc gggtgaacgc tcagcctcgc cgatcgcgag gcgatagact
     3181 gcgatgactt cagggcgcgc cgcctcgctc agcaacgagg cgccgaacgt catgagtgtc
     3241 gcgagcaaac cgtcgcggtc gcgcggcgcc ggcagctgca ggggcaggcg catgcgctcg
     3301 gcacgttcgg cgatgcaggc ttcgagcatg gcgtgcttgc taccgaactc ggcatagaga
     3361 tcgcgcttcg acaccttggc acggctcgcg atgtcgagcg tgctggttcc cgcgtaaccg
     3421 cgctgcatga actcctcgaa agcggcgcgg aggatgcggc gccgcaacgt gacgggcccg
     3481 tcgtcggtct gtctggtgcg cggcatcaaa cccctcttgc gctggtaccg tatggtaccg
     3541 tataagcggt accagatagt accacggtca tggaattgtt tccagatgag caacgcagct
     3601 ccggttgccc ggctcgatca tgtcgggaag acgtatcatg ccgggaagat cgatgtcgcc
     3661 gcgatccggg acatcagcat ggcgatcccg acgcagcgct tcagcatgat catcggtcct
     3721 tccgggagcg gaaagacaac actgctgaac ctgatcggct gtatagacac gccgacggcc
     3781 gggcggatcg aagtgtgcgg cgaggatatc gggcgccttt cggacaatgc gctggcggat
     3841 ttccgcgccc gcaacatcgg cttcatcttc caggggttca gcctggtgcc ggtgctctct
     3901 gcgtatgaga acgtggagta tccgctgatg ttggttggca tggcgacggg cgaacgccgt
     3961 cggcgcactg tcgctatgct cgatgccgta gggctgtcgg aacaggcgaa ccagcggccg
     4021 aatgaattgt ccggcggcca gaagcagcgc gtggcgatcg cgcgtgcgct ggtcaagcag
     4081 ccgaatctgg tgctggccga tgagccgacg gccaacctgg ataccgcgac gggagccgcg
     4141 atcatcgcgc tgatgcggcg catgcaggcc gagcagaaca cgacgttcat cttctcgacg
     4201 catgatccgc agctgatttc gcattccgag gagaccttca cgatccgcga tggcgttctg
     4261 atgtcgcacg cgacggaaag gagcgactga tgcttttcag gatcgccttc cgcaacatcc
     4321 tgcgcaatcg ccggcgttcg ctgatgaccg gttcggcagt tgcggtcggc acgctggcga
     4381 tgctgctgtt cggctgcttc atcggctaca tctttgcagc actcgagacc ggcctggtgc
     4441 agcgtgtcgg ccatctgagc gtgttccgca gtggctattt cgtgttcggc gcaggcaatc
     4501 catcggctta cgggatcgac gattatcgcg gtgtgatgcg catgattggc ggcgatccgg
     4561 tgctgaagcc gatggtgcgc gtgttgacgc cgaggcaatc actcgtgggc atcgccggca
     4621 atttttcggg cggcaacgat gcggcgaaga cgttcctcgg tatcggcctt gtgccgtccg
     4681 acaggtcgac catgcgggaa tggaatgagc atgggacggc gccgttgtat gtgccggatg
     4741 acaggatgtc ggacgacgcg ccgtcacgcg ggctgatcgg cggcggcatg gcgcgcatcc
     4801 ttgggctgtg cgcgccgttg aagctggccg attgccctgc gatgccgaag cacgacgagc
     4861 catcgtcaga gcatgcgcca gcggcgcagg acctgagcag tctcgcccgc gccgaacttg
     4921 gcagccaggg accggcaaaa acgacgccgc aactggatct gctgagtgcg actgcggcgg
     4981 gtgcgccgaa tgtcgtcagt atggatgtgg caggcgtgga tctgcagagt gccaaggagc
     5041 tcgacgacgc ttacgtcgcg atgcctctgc cgctggcgca gcagcttgtg tatggccgtg
     5101 gcgagcacaa agtcaccggc atcgtgctgc agctacagcg tacagaggat ctggcagcgg
     5161 cacgcgcacg gctggagacg ctgatcggcg agcatcatct cgatctcgag gtgcatgatt
     5221 ttgctgagct gacaccgttc tatgttcagg tggtcaggct gttcggcacg atcttcctgt
     5281 tcatcgcgct gatcatggca atcatcgtgc tgttcgccgt ggtcaatacg atgaccatga
     5341 acgtggtgga acgcacgaac gagatcggga cgatccgcgc gatgggcgtg cggcggaacg
     5401 gcatccgcgc gcagttcgtg ctggagggct ggttgctcgg agcgatcttc gcggcagtcg
     5461 gggtggtgct ggcgttcgtg gtcgcaacgg cgatcaacca tgcagggctg acctggagcc
     5521 cgccgggcaa cgccaatgct gtgccgttcc gactcgcgat cccgagcagc cggccgctgc
     5581 tgttggccgg cacgtggatc gggctc
//
LOCUS       DATGEM010000674         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_412165, whole genome shotgun sequence.
ACCESSION   DATGEM010000674 DATGEM010000000
VERSION     DATGEM010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412165"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..652
                     /locus_tag="VKQ27_20830"
     CDS             65..652
                     /locus_tag="VKQ27_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91440.1"
                     /translation="MRRLGAVVVLAGAAALLSGCVYDPYTGSYYPCCSYYGNPYYRYP
                     PAYYPPPQGYPQGYPSGQPQGYPSGQPQGYPSGQPQGYPPGQSQGYPSGQPQGYVPGQ
                     EQGSYQAQPLPPPAPQPGASAYPQGGGGGLVQRFATANVTHDGRLTREQAESGMPMVA
                     RNFDTIDIEHKGYITLPEIRAFAAERRAAGQSATE"
     gene            761..1522
                     /gene="lepB"
                     /locus_tag="VKQ27_20835"
     CDS             761..1522
                     /gene="lepB"
                     /locus_tag="VKQ27_20835"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLY91441.1"
                     /translation="MATKVKAKAKRKSNVWVENIKTIVYAGLIAVVVRTVAYEPFNIP
                     SGSMIPTLLVGDYLFVSKYSYGYSRYSLPFGWPPFEGRIFGQLPRRGDVAVFKYPRDN
                     TTDYIKRIVGLPGDHIQMKQGVLYINGQPCPRQPEGNFDFTDDSGIRMSVQRYQETLP
                     DGVKHDILKETDDGPVNNTQDYLVPPGHVFAMGDNRDNSADSRFMNGVGFVPVENLVG
                     HAEIIFFSVDADEPWWEIWWWPFEIRWSRLGRIIH"
     gene            1519..2208
                     /gene="rnc"
                     /locus_tag="VKQ27_20840"
     CDS             1519..2208
                     /gene="rnc"
                     /locus_tag="VKQ27_20840"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HLY91442.1"
                     /translation="MNAPDPAASLEAILGHAFARPELLREALTHRSAVHGRGRRGRGS
                     NERMEFVGDRVLGLLIAEWLAERFPHEQEGDLGRRLALLVSQPVLAEVAEAIGLGEAL
                     SVSPGEAKAGVRRRATVLADALEAALGALYLDGGLERARAFVRSAWDTVMSRQEEPPK
                     DSKTRLQEWVQKRGLGLPEYDVMSRAGPPHAPEFVVTVAAGGFAGTGSAGSKRAAEQL
                     AAAELLRMLGA"
     gene            2259..3158
                     /gene="era"
                     /locus_tag="VKQ27_20845"
     CDS             2259..3158
                     /gene="era"
                     /locus_tag="VKQ27_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLY91443.1"
                     /translation="MGDATRCGFVAVVGEPNAGKSTLLNRLTGAKLSIVTPKAQTTRF
                     RVLGILMHGGSQVLLVDTPGIFAPKRRLDRAMVAAAWTGVRDADLTLLLVDAKAGVTE
                     GVRAIVAALMEQKRRCWLVLNKVDLVPAQSLLPLTAALIALAPFEQTFMVSAVTGDGV
                     TALAEALAAAVPEGPHLYPDDDLTDLPERLLAAEIVREQVFLQTHEEVPYATTVETEN
                     WQDRPDGSVRIDATVYVARPGHKAILIGERGQRIKTIGARAREQLATLLERKVHLFLN
                     VKQRAGWDEETARLRAIGLDDPG"
     gene            3169..3624
                     /locus_tag="VKQ27_20850"
     CDS             3169..3624
                     /locus_tag="VKQ27_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLY91444.1"
                     /translation="MTGLYFEEFHEGQVFEHSLSRTVTEMDNVLFSSLTLNPQPLHLD
                     EEFAKTTEFGQRIVNSLFTLGLMIGMTVGDTTLGTTIANLGMNKVEFPKPVFHGDTIR
                     AKSIVRSVRASRSRPEAGVVEFEHQAFNQRGELVATCLRAAFMKRRARA"
     gene            complement(4072..>4460)
                     /locus_tag="VKQ27_20855"
     CDS             complement(4072..>4460)
                     /locus_tag="VKQ27_20855"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HLY91445.1"
                     /translation="LQPAVSAWSATELDALGRTEEIEIAALRADGTLRKPVIIWIARL
                     DDGLYVRSAYGRTAAWYRGTLARHEGRIKAGGVEKDVTFEAAELALHDAIDTAFRTKY
                     RAHPVQYVNMVLTEEARSTTLRLNPR"
BASE COUNT          775 a         1339 c         1564 g          782 t
ORIGIN      
        1 tacccgtacg cgttcgccca ggtgatcatt tgggcgatgc cggagcggta aaggagagca
       61 aaccatgcga cgccttggtg ctgtcgtcgt gcttgccggt gcggcggcgc tgctgagtgg
      121 ctgcgtctat gatccctaca ctgggtcata ctatccgtgc tgctcgtact acggcaatcc
      181 gtactaccgt tatccaccgg cgtattaccc gccaccgcag ggttatccgc agggctatcc
      241 gtcgggacag ccgcagggct atccgtcggg acagccgcag ggttatccgt cgggacagcc
      301 acagggttat ccgccaggac agtcacaagg ttatccgtcg ggacagccgc agggctacgt
      361 gccggggcag gagcaaggtt cgtatcaggc ccagccgctg ccgcccccgg cacctcagcc
      421 cggcgcctcg gcctatccgc agggcggtgg tggcggcctc gtacagcgct ttgccaccgc
      481 gaacgtgacg catgatgggc ggttgacccg cgagcaggcc gagtccggca tgccgatggt
      541 tgcgcggaac ttcgacacca tcgacatcga gcacaaaggc tacatcaccc tgcctgaaat
      601 ccgcgctttc gcggccgagc ggcgtgccgc tggtcagtcg gcgaccgaat agcgcttgta
      661 cgttgctgtt ttgtcaggtt gccgccgccg ggcgctcgct tgacacacca cacgcccatg
      721 ggcttgatcc gcgcgttttt acacggaagc cgcttgccct atggcgacca aggtcaaggc
      781 gaaggccaaa cgcaaatcca acgtttgggt ggagaatatc aagactatcg tctacgccgg
      841 cctgatcgcc gtcgtggtgc gcaccgtcgc ctatgagccg ttcaatatcc cctcgggaag
      901 catgatcccc acattgctgg tgggcgatta cctgttcgta tcgaaataca gctacggcta
      961 ttcgcgctac tcgctgccgt tcggctggcc accgttcgaa ggccgcatct tcggacagct
     1021 gccgcggcgg ggcgacgtgg cggtgttcaa gtacccgcgt gacaatacca ccgactacat
     1081 caagcggatc gtcgggctgc cgggcgacca tatccaaatg aagcaggggg tgttgtacat
     1141 caatggccaa ccctgcccgc gccagcctga agggaatttc gacttcaccg atgacagcgg
     1201 cattcgcatg tcggtgcagc gttaccagga gacgctgccg gatggcgtaa agcatgacat
     1261 cctgaaggaa actgacgacg gtccggtcaa caacacccag gactatctgg tgccgccagg
     1321 gcatgtcttc gcgatgggcg acaaccgcga caactctgcg gacagccgtt tcatgaacgg
     1381 cgtcgggttc gtgccggtcg agaatctggt aggacacgcc gagatcatct tcttctcggt
     1441 cgatgcggat gagccgtggt gggaaatctg gtggtggccg tttgagatcc gctggtcgcg
     1501 tctcggcagg atcattcact gaacgcgcct gacccggctg cgtcgctcga ggccatcctt
     1561 ggtcatgcgt tcgcccgccc cgagctcctc cgcgaagcgc tgacccaccg atcagcggtg
     1621 catgggcgcg ggcggcgggg ccgcggctcc aacgagcgga tggaattcgt cggcgaccgt
     1681 gtgctgggac tgctgatcgc agaatggctg gcggagcgct tcccgcacga acaggaaggc
     1741 gatctggggc ggcggctggc cctgctggtg tcacagccgg tgctggccga ggtggccgag
     1801 gcgatcgggc tcggcgaggc gctgtcggtg tctccgggcg aggcgaaggc tggcgtgcgg
     1861 cggcgcgcta cggtgctggc ggacgcgctg gaggcggctc tcggagcgct ctatctggat
     1921 ggcgggctgg aacgcgcgcg ggccttcgtg cgaagcgcct gggacacggt gatgagccgg
     1981 caggaggagc caccgaaaga ttccaaaacc cggttgcagg aatgggtgca gaagcgcggg
     2041 ctcggtctgc cggagtatga tgtgatgtcg cgcgccggac cgccgcatgc cccggagttc
     2101 gtggtgacag tggcagccgg cggtttcgcg ggtaccgggt cggcgggcag caagcgggcg
     2161 gcagagcagt tggcggcggc agagttgctg cggatgcttg gagcataatg ggccagcccc
     2221 ccacctcacc gagccctccc cgccctgcgg gggccggagt gggcgatgcg accagatgcg
     2281 gcttcgtcgc cgtggtcgga gagccgaatg caggcaagtc cacgctgctg aaccggttga
     2341 ccggcgcgaa gctgtcgatc gtgacgccca aggcgcagac aacgcggttc cgcgtgcttg
     2401 gcatcctgat gcacggcggc agccaggtgc tgctggtgga cacgccaggc atcttcgcgc
     2461 cgaagcggcg gctggacagg gcgatggtgg cggcggcatg gaccggcgtc cgcgatgcgg
     2521 acctgacgct gctgctggtg gatgcgaagg ccggcgtgac cgagggcgtg cgcgcgatcg
     2581 tcgcggctct gatggagcag aagcggcgct gctggctggt gctcaacaag gtggacctcg
     2641 tgccggccca gtcgctgctg ccgttgaccg cggcattgat tgcgctggcg ccgtttgagc
     2701 aaacgttcat ggtcagcgcg gtcacaggtg atggcgtgac ggcactggct gaagcactcg
     2761 cggcggccgt gcccgaaggt ccgcacctct atcccgacga cgacttgacc gacctgccgg
     2821 agcgcctgct ggcggcggag atcgtgcggg agcaggtctt tctccagaca cacgaggaag
     2881 tgccctacgc cacgaccgtg gagaccgaga actggcagga ccggccggac ggctcggtgc
     2941 gcatcgatgc gacggtgtat gtcgcccgac ccgggcacaa ggcgatcctg atcggcgaac
     3001 ggggccagcg gatcaagacg atcggggctc gggcgcgcga acagctggcc acgctgctgg
     3061 agcgcaaggt gcatctgttc ttgaatgtga agcagcgcgc aggttgggac gaggagacgg
     3121 cccggctgcg cgcgatcgga ctggacgatc cagggtagga attcaccaat gacaggtctc
     3181 tatttcgagg agtttcacga ggggcaggtc ttcgagcatt cgctgagccg tacggtgacc
     3241 gagatggaca acgtgctgtt cagctcgctg acgctgaatc ctcagccgct gcacctggat
     3301 gaggagttcg caaagacgac cgagttcggg cagcggatcg tgaactcgct gttcacgctg
     3361 gggctgatga tcggcatgac cgtgggcgac accaccttgg ggacgaccat cgccaatctc
     3421 ggcatgaaca aggtggagtt cccgaagccg gtgttccatg gcgatacgat ccgggcaaag
     3481 tcgatcgtgc gctcggtgcg ggcatcgcgg tcgcggccag aagccggcgt ggtggagttc
     3541 gagcaccagg cgttcaatca gcgcggcgag ctggtggcaa cgtgcctgcg ggcggcgttc
     3601 atgaagcgga gagcacgagc gtaggcgccg cctgttacat tgatccggac gctggcgggc
     3661 catatcacga tgtggtcgcc agcccgtgga gaagcagcgt tacgggttct cgtccggtga
     3721 tgcgctgggg acggcccacg aattactgcg ggccgtgctc tggaagccgc agcgcgacat
     3781 cgaaggagtg ctgatcgagc gccactcatg gagcggtggg cttatgcacc gtcgcgctga
     3841 tggcgggttg gttctgacgg agcatcattg gcacctgcgc catgcggttg acgtcagcgg
     3901 gccactcctc aacggagata ggcgatctct gatcaccgat tgtcgagcaa agccaaacct
     3961 tcggtgctga tgcgcgtccc gctgaagggc atcgcgcaga tcgtgcagca gcagcctccg
     4021 gccgccgagc agcgcgacac cagggccaga ccggccgagg atgagacgca gtcagcgcgg
     4081 gttgagcctc agtgtcgtgg atcgcgcctc ttctgtgagc accatgttca cgtactggac
     4141 gggatgggcc cgatatttgg tgcggaaggc cgtgtcgatg gcgtcatgca gcgcgagctc
     4201 ggcagcctcg aaggttacgt ccttttcgac accaccggcc ttgatgcggc cttcgtgacg
     4261 cgccagtgtg cccctgtacc aggcggcagt gcggccatag gcggaacgga cgtagagacc
     4321 gtcatccagc ctggcgatcc agatgatgac cggcttgcgc agcgtgccat cagcgcggag
     4381 ggcggcgatt tcgatttcct cggtgcggcc gagcgcatcg agttcggtcg cgctccaggc
     4441 gctcacggcg ggttgtagcc
//
LOCUS       DATGEM010000675         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_373638, whole genome shotgun sequence.
ACCESSION   DATGEM010000675 DATGEM010000000
VERSION     DATGEM010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373638"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VKQ27_20860"
     CDS             complement(<1..597)
                     /locus_tag="VKQ27_20860"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLY91446.1"
                     /translation="MMYFTPLKTAVILGLCLLGAILCIPNLFPAPAAWLPWRQVHLGL
                     DLRGGSYLLLEVDMKSVTKERLESTVDGIRQALRPGAIFYQSLDAQPDQNRILLRLRD
                     ATKLDAALAAIRPLINTEGPTNTPDFQIASSPDGTIILTLSQVALNARALGAVQQSIE
                     IVRRRIDETGVVDPQIAQQGDSRIVVQLPGISDPNRIKE"
     gene            738..1421
                     /locus_tag="VKQ27_20865"
     CDS             738..1421
                     /locus_tag="VKQ27_20865"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLY91447.1"
                     /translation="MHIGLIGGIGPAATEFYYRGLIDRHAKAGTKLDLTIAHADVREM
                     AANLTAGAAQTQAEIFARLLRRLAAAGAGSAAITSMGGHFCMRELLAISPLPLVNAIP
                     EVDAAIRARGLRTVGIIGTRVVMETGLYGGITAARIVRPEDDALDEVHTAYVTMAGEG
                     RVAEAQRRVFFAAGRQLCQAQGAEAVVLGGTDLFLAFAEEDCGFPTLDCADIHVDALY
                     KRSVADQPA"
     gene            complement(1409..1741)
                     /locus_tag="VKQ27_20870"
     CDS             complement(1409..1741)
                     /locus_tag="VKQ27_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91448.1"
                     /translation="MALGTQVSGASITSMIRLAAESQNSVAKRDCALIMVHALGIPGL
                     IPAKDSTDVCILIEGALRAVLLRCGYPFSGTVPEKLAVLQRLHRTIGELMQPYEPTFP
                     NWQGLYAG"
     gene            2161..3162
                     /locus_tag="VKQ27_20875"
     CDS             2161..3162
                     /locus_tag="VKQ27_20875"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLY91449.1"
                     /translation="MSSFELVRPLPNASFGGLICRAGNAHALVAAAEAESGSLPRALA
                     EAGGLLLIEGMDAMADAPELLVRLSRIFGPEVEDYRNTLTPLNMVHRTVPEIFVVANK
                     PPVSRPPPRRPDPPLTAEGKLPVQFPHRRGWHTDQSYRRPPPDISLFLAVHPVPQGQG
                     QTMFADGAAAYEALPPALKARVDTLEGLHVGMSAKRGRAAVLDGEAPRALQPHEQPQR
                     QPLARVHPVTGKRALYMCEYGQMDWLEGPIVGMEPGPHGAGGKLLEGLMSHMTQPRFV
                     YVHEWTQGDLVVWDNRCLVHAATWFDADSLAREMWRTTVFGNPGATYAGESKSWVAA"
     gene            complement(3249..3947)
                     /locus_tag="VKQ27_20880"
     CDS             complement(3249..3947)
                     /locus_tag="VKQ27_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91450.1"
                     /translation="MPVIASAEDVRATRRRASSNGTGFWLTSYIGANRYSRGAEEPPG
                     PGTIYPMAFLVEQDPDAVVGAHYHQADQFQVMVDGTGRLGTHAVAGGAVHFAGAWTAY
                     GPLAAGQGGLHYFTLRNGWDPGARYMEFPDNRATLRSLPRQHREAVGEVTATSDGLAA
                     RRHQLDAGASVTGPDPIESGGQFWLVLAGSMQRDGGALPPRSCIFIYPHEPAFTAISG
                     PEGLTVLMLQFPKA"
     gene            3995..4396
                     /gene="hisI"
                     /locus_tag="VKQ27_20885"
     CDS             3995..4396
                     /gene="hisI"
                     /locus_tag="VKQ27_20885"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HLY91451.1"
                     /translation="MSEHDILEAIRFDSAGLVPVIAQQHDTGEVLMLAWMNCAAVAET
                     LATRRVCYFSRSRGALWRKGETSGQVQHLKELRVDCDGDTLLLLVDQEGVACHTGRRN
                     CFFRAVRDGDLVEIAAPLVPPEALYGGHSHS"
     gene            4374..5426
                     /locus_tag="VKQ27_20890"
     CDS             4374..5426
                     /locus_tag="VKQ27_20890"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91452.1"
                     /translation="MAAIPIADPEHLTAVLRRAGVLGDASVRDVTVESDQPTIVSRIV
                     RLRLHFDGDSGDAPTSIILKTAHPERLASSWGAGRNEVAFYRDVATAMPSGTVPRCFE
                     AHADDATKSWHLLLEDLTDSHMIVASWPLPPSLTQCERIVEARARFHAVWWDEARLGR
                     SIGVRRDARMVEQTMRDFGSHYVRMAARLGDGLSEEQRALYEQFQRATTPLIARYETR
                     RHLTITHNDAHVWNCFMPRDGGSDVRLFDWDSWRIGLGASDLAYMMAVHWYPEWRRRM
                     ESALLDRYHATLVECGVQGYDRQALDDDYRLAVLWQIATPVWQAAYNIPPVIWWNNMQ
                     RVLLAVDDLGCRKWLA"
     gene            5461..>6286
                     /locus_tag="VKQ27_20895"
     CDS             5461..>6286
                     /locus_tag="VKQ27_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HLY91453.1"
                     /translation="MPLIYLVAGEASGDALGARLMAAITARRDDVAFAGIGGEQMAAK
                     GLTALFPMRDLALMGLLEVLPRLLQLRQRLRQTAEDIAARRPDVVVTIDSPGFTLRLL
                     KMVQSLGVKRVHYVAPQVWAWRESRVRHYPGLWDQLLCLLPFEPAFFARHGLPAVFVG
                     HPVLESRADRGSAARFRSSHGIGTDGRVLTLMPGSRRMEVSRLLPVLGETLRKLPDDV
                     VPVVPLAGPVAKTVDEGTRRWRRKPILIGDERDKYDAFAASSVALTKSGTSTLELAM"
BASE COUNT         1058 a         1837 c         2217 g         1174 t
ORIGIN      
        1 ttccttgatc cggttagggt cgctgatgcc ggggagctgc acgacaattc gagagtcgcc
       61 ctgctgtgcg atctggggat cgacaacacc cgtttcgtcg atgcgccggc ggacgatctc
      121 gatcgactgc tggacggcgc cgagggcccg ggcgttaagg gccacttgcg aaagcgtgag
      181 gataatcgtg ccgtcgggcg aggacgcaat ctggaagtcg ggggtgttgg tggggccttc
      241 ggtattgatc aggggccgga tggcggcgag cgcagcatcc aacttggtgg cgtcgcgcag
      301 gcggagaagg atgcggttct ggtcgggctg tgcgtccagc gactggtaga aaattgcacc
      361 ggggcggagg gcctggcgga tgccgtccac ggtgctttcc aaacgctcct tggtgacgga
      421 cttcatgtcc acctcgagaa ggaggtagga gcccccgcgc aggtcgagac ccaggtggac
      481 ctggcgccag gggagccacg cggcgggtgc gggaaacaaa ttgggaatac agaggatcgc
      541 acccagcagg cacaggccga ggatgaccgc ggtcttcagg ggcgtgaaat acatcatggg
      601 gtggcggctc gtccctcctg gcgaggcgcc cctatgcgcc aggggcgtgc gcaggtccag
      661 atagaggatg agaggacgga ttccggcata cgcgaaccgg tggcatgctt tgccgcgacc
      721 acatccggga tgacccaatg cacataggtc tcatcggcgg catcgggccc gccgctaccg
      781 aattctatta ccgtggcctg atcgaccggc acgcaaaggc gggaactaag ctcgacctga
      841 ccattgcaca tgccgacgtg cgggagatgg cggccaatct gacggcaggc gcggcgcaga
      901 cgcaggcgga gatattcgcc cggctgctgc ggcgtctggc cgcggcgggg gctggatcag
      961 ctgccatcac ctcgatggga gggcatttct gtatgcgcga gctactggcg atctcgccac
     1021 tgccgctggt caatgccatc ccggaagtgg atgccgcgat tcgcgcgcga ggcctgcgga
     1081 cggtgggtat tatcggcacg cgcgttgtga tggagacggg actctacggc gggatcactg
     1141 ccgcgcggat tgtgcggcca gaggatgatg cgctggacga ggttcataca gcctatgtca
     1201 caatggcggg ggaaggccgg gtcgccgaag cacagcgccg cgtgttcttt gccgctggcc
     1261 ggcagttgtg ccaggcgcaa ggtgccgagg cggtcgtgct cgggggaacc gacctgtttc
     1321 tagccttcgc cgaggaagac tgcggatttc ccactctgga ttgcgccgat atccatgtgg
     1381 atgcgcttta caagcgttcg gtcgcggatc agcccgcgta gaggccctgc caattgggga
     1441 aggtcggctc gtaaggctgc atcaactcgc cgatggtgcg atggaggcgt tgcaacacgg
     1501 ccagcttctc cggcaccgtt ccgctgaacg ggtaaccgca gcgcaataac accgcgcgca
     1561 aggcgccttc gatcaggata cagacatcag tactgtcctt tgccgggatc agcccaggga
     1621 tgccgagcgc gtgcaccata atcaacgcgc agtcgcgctt cgccaccgaa ttttgtgact
     1681 cggcagccag acggatcatc gaggtgatcg aggcgccact tacctgggtg ccaagcgcca
     1741 agcagcactg ctccgcgagg acgtggcgcg ccgcgcgcaa tgccgcgtta cggtcttttg
     1801 cggcgttggc ctgggacgct gccaccgcca gccgcctgag cgcagcctct acatccattt
     1861 ggcctctcta tccgacacga tttttctcga caaatcattg gctcattccc tcgacggcgc
     1921 actgatccgc ccgctgcgcg ccagtaccgc cagcttcacc tgactgacct gcatcagatt
     1981 gccttgccgc atggcttcct tccggtcggc ctcgctgagc cggcacagcg catggggcgg
     2041 tgactcgcaa ccagtgcaga ttgccctgat cgcgaccgaa catgctcccg agcagaccag
     2101 gttgaccgct caacatctct cgctgaaatt ggggccttcc acgccgcaag caggtgccgc
     2161 ttgagcagct ttgaactcgt ccgacccttg ccaaatgcca gttttggcgg gctgatctgt
     2221 cgtgccggga acgcgcacgc gctggtggcc gccgccgagg cggaatctgg cagccttccg
     2281 cgtgcgttgg cggaggcagg tggtctcttg ctgatagagg gcatggatgc gatggcggac
     2341 gcgcccgaac tgctggtgcg gttgtcgcgg atcttcggcc cggaggtcga ggattaccgg
     2401 aacacgctga cgccgctcaa catggtgcat cggacggtgc cggagatatt tgtcgtggcg
     2461 aacaagccgc cggtgtcgcg ccctccgcct cgacgcccgg acccgccact gacggctgag
     2521 ggcaaattgc cggtgcagtt tccgcatcgg cggggttggc acacggatca gagctatcgg
     2581 cgaccaccgc cggacatctc actgttcctg gcggtgcatc cggtgccgca ggggcaaggg
     2641 cagacgatgt tcgccgatgg cgcagcagca tacgaggcgc tgccgccggc actgaaggcg
     2701 cgggttgaca cgctggaggg gctgcatgtg ggaatgtcgg ccaagcgtgg gcgggctgcg
     2761 gtgctcgatg gcgaggcgcc gcgggcgctc caaccgcatg agcagccgca gcggcagcct
     2821 ttggcaaggg tccatccggt gacgggcaag cgcgcattgt atatgtgcga gtatgggcag
     2881 atggattggc tcgaggggcc gatcgtgggt atggagccgg gcccgcacgg cgctggcggc
     2941 aagctgctcg aagggctgat gtcgcacatg acgcagcctc ggtttgtgta tgtgcacgag
     3001 tggacgcagg gcgacctggt ggtgtgggat aatcgctgcc tggtgcacgc ggcgacctgg
     3061 ttcgatgccg acagcctggc acgcgagatg tggcggacca cggtgttcgg caatccgggt
     3121 gcgacttatg ccggggaaag caagagctgg gtggctgcct gatcggttgg tgacgagcct
     3181 gatccagctc ggttcgggct catcgtacct gatggtgcat gaaatgcagg ccggcgtggt
     3241 cagtgggctc aggccttggg aaattgcagc atcagcactg tgagaccttc tgggccactg
     3301 atcgcggtga aggcgggttc gtggggatag atgaaaatgc aggagcgtgg cgggagcgcc
     3361 ccgccgtccc gctgcatgct gccggcgagc accagccaga actggccgcc gctttctatg
     3421 ggatcgggcc cggtgacgga cgcccctgca tccagctggt gacggcgggc agcgagaccg
     3481 tcagaggtgg cggtgacctc gcccaccgct tcccgatgct ggcgcgggag cgagcgcagc
     3541 gtggcgcggt tgtcggggaa ttccatgtag cgagcgccgg ggtcccagcc gttgcggagg
     3601 gtaaagtagt ggaggccccc ctgccccgcc gccaacgggc cgtaggcagt ccaggcgcca
     3661 gcgaagtgga ccgcccctcc ggctacggcg tgggtgccga ggcgccccgt cccgtctacc
     3721 atgacctgga actggtcggc ctgatggtaa tgcgcgccga cgacggcatc ggggtcctgc
     3781 tcgaccagga atgccatggg atagatagta ccgggaccgg gtggttcctc ggcgccgcgg
     3841 ctgtagcggt tggcgccaat gtagcttgtg agccagaagc cggtgccgtt ggagctggcg
     3901 cgccggcgtg tggcacggac atcctcggcc gaggcgatca ctggcattgt ccggcacctt
     3961 tgcccacgaa cgccatgccc ccaagatagc aggcatgagc gaacacgaca tccttgaagc
     4021 catcaggttt gacagcgccg gcctggtccc cgtcattgca cagcagcacg acaccggcga
     4081 ggtgcttatg ctggcgtgga tgaattgcgc ggccgtggcc gagacactgg ccacgcgtcg
     4141 ggtgtgctac ttcagccgca gccgtggggc gctgtggcga aagggagaga cgtcgggcca
     4201 ggtgcagcat ctgaaggagc tgcgcgtcga ttgcgatggc gacacgctgc tgctgctggt
     4261 ggatcaggaa ggcgtggcct gtcacactgg gcggcggaac tgcttcttcc gggcggttcg
     4321 ggatggcgac ttggtggaga tcgccgcgcc gctggtccca cctgaggcat tgtatggcgg
     4381 ccattcccat agctgatccg gagcatctga cggctgttct gcggcgcgcc ggcgtgctcg
     4441 gtgatgcgtc ggtccgcgat gtaacggtgg agagcgacca acccacgatc gtgtcgcgca
     4501 ttgtccgttt gcgcctgcac tttgatggcg acagcggtga tgcgcccact tccatcatcc
     4561 tgaagacggc gcaccccgag cgcctcgcca gcagttgggg agccggacgc aacgaggtcg
     4621 cgttctaccg tgatgtggcc actgccatgc cgtcaggaac ggtgccgcgg tgctttgagg
     4681 cgcatgccga tgacgcgacg aagtcctggc acctgctgct ggaggatctg accgactctc
     4741 atatgatcgt tgcctcatgg ccattgccgc cgtcgctcac gcagtgcgag cgcatcgtcg
     4801 aagcccgtgc gcgcttccat gcggtgtggt gggacgaagc gcgccttggt cgatcgattg
     4861 gcgtacgtcg cgatgctcgg atggtcgagc aaacgatgcg ggacttcggc agtcattatg
     4921 ttcggatggc cgctcggctt ggcgatggcc tgtcggagga acagcgggcg ctgtacgagc
     4981 agtttcagag ggccaccacg ccactgatcg cacgctacga gacgaggcgc cacctgacca
     5041 taacgcataa cgacgcacat gtctggaact gcttcatgcc gcgtgatggt ggcagtgatg
     5101 tacggttgtt cgactgggac tcctggcgca tcggcctggg cgcgagtgat ctggcctata
     5161 tgatggccgt gcattggtat ccggagtggc ggcggcgcat ggagagtgcg ctgctcgatc
     5221 gctatcacgc gacgctggtc gagtgtggcg tgcagggtta cgaccgtcag gcgctggacg
     5281 atgactatcg gttggcggtg ctgtggcaga tcgccacgcc ggtctggcag gccgcgtata
     5341 acattcctcc cgtgatctgg tggaacaaca tgcagcgtgt cttgttggca gtggacgatt
     5401 tgggctgccg caagtggctc gcgtaagaag ggtccgcatc tgcggttcca tattcttccc
     5461 atgccactga tctatctggt tgctggcgag gccagcggtg atgcgcttgg cgctcggctg
     5521 atggccgcga tcaccgcacg gcgggacgat gtggcgttcg ccgggattgg cggcgagcaa
     5581 atggccgcga agggtctgac ggcactgttc ccgatgcggg atctcgcctt gatggggctg
     5641 ttggaagtgc tgcctcggct gctccagttg cgccaacgat tacgtcagac cgcggaggac
     5701 attgccgctc ggcggccgga tgttgtcgta accatcgaca gccctggatt tacgctgcgc
     5761 ttgctgaaga tggtccaatc tctcggggtc aagcgtgtgc attacgtcgc accgcaggtg
     5821 tgggcgtggc gtgaaagccg cgtgcggcat tatccgggac tgtgggatca gttgctgtgc
     5881 ctgttgccgt tcgagcccgc gttcttcgca cggcatgggc tcccggcagt attcgtcggt
     5941 catcctgtgc tggaaagtcg cgccgatcgc ggcagcgcgg cccgatttcg atcgtcacac
     6001 ggcattggca cggatggccg tgtattgacg ctgatgccag gcagccgccg catggaagta
     6061 tcgcgattgc tgcccgtcct cggtgagaca ttgcggaagc tgcctgacga cgtggtgccg
     6121 gtggtgcctc ttgctgggcc ggtcgccaag acggtggacg agggaacgcg gcgatggcgg
     6181 cggaaaccga tccttattgg cgacgagagg gataagtacg atgcgttcgc tgcttcgtcg
     6241 gtagcattga ccaaatcggg aacctcgacg ctcgaactgg cgatgg
//
LOCUS       DATGEM010000676         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_964375, whole genome shotgun sequence.
ACCESSION   DATGEM010000676 DATGEM010000000
VERSION     DATGEM010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964375"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VKQ27_20900"
     CDS             complement(<1..183)
                     /locus_tag="VKQ27_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91454.1"
                     /translation="MSRLNNIFGDLFARRDVPSLPPPQVSMSEVVRDRMNRALRRRLS
                     DRVMDVFNESCIAGDLD"
     gene            complement(214..402)
                     /locus_tag="VKQ27_20905"
     CDS             complement(214..402)
                     /locus_tag="VKQ27_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91455.1"
                     /translation="MPVPEYPPGTRAPVGGVYEQRNVLGAPTGEQVAMAPGEPLPAAP
                     RGFTWRLVEARKDDGTVN"
     gene            complement(558..1898)
                     /locus_tag="VKQ27_20910"
     CDS             complement(558..1898)
                     /locus_tag="VKQ27_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY91456.1"
                     /translation="MNLRLWPRSLKARTAVVLLVGLAVVQVAGLAIHALDRIDVQRLA
                     QAREIAVRVVSVYRTVMMTSPDRRQTVLAEIHREPGLAAEIDEDPPTEDLPDEVPFGQ
                     RLLRVNLNLVPLLGAQHWRGLRILTDASHHIIVIGMRMPPEGEWLKVTVPYEPPRPWH
                     SPTFLAAFLVMTLAAAGLTLWAVRRLTAPVSTLAAAAEALGRDVNAPPLPEDGPTEVA
                     TAAAAFNTMAARIRRFVQDRTELLTAIGHDLRTPITRLKLRAEFVEDDEQRNKMLSDL
                     EELEAMVSATLAFGRDAQTTEPVSPLDLAELLRTVLDEAGDAWPDLATRLAYEGPPHF
                     TMRARPLALKRALTNLVSNALAYGGSARIRMVPPIDGTLVIEVEDDGPGIAPGELERV
                     FEPFHRGEPSRNRETGGVGLGLPIARNIFRAHGGDVTLANRPMGGVKATITLPV"
     gene            complement(1895..2617)
                     /locus_tag="VKQ27_20915"
     CDS             complement(1895..2617)
                     /locus_tag="VKQ27_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY91457.1"
                     /translation="MEHVPHILVVDDDREIRDLLARFLEEKHHMRVTAVRDGREARRA
                     FANGHYHLVVLDLMLPGEGGLDLARWLRTQSDVPIVMLTAMGEETDRIIGLELGADDY
                     VPKPFNPRELLARIRAVLRRVGDGADHRPDANARSLKFAGWTLEPGRRRLLNPDGAEV
                     ALTGGEYDLLVALVDRANRVLTRDMLLDLLRGRQAGPFDRAIDVAISRLRRKLEDDGR
                     NAQLIKTVRGGGYVLAAMVERV"
     gene            2764..3189
                     /locus_tag="VKQ27_20920"
     CDS             2764..3189
                     /locus_tag="VKQ27_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91458.1"
                     /translation="MRRTILATVAVLAIGGGTAGVLIAQAQPAPSPQTADAPSPPQRP
                     HWMGWMHRGRDGHGGPWAAMRRNFALVYRHEDRQLAPPDVQKIAEAFLLWNGNHTWKV
                     ANVKPVSDGTIGFDLTTASGDVIASFTMDTHTARVTRAG"
     gene            complement(3253..>4286)
                     /locus_tag="VKQ27_20925"
     CDS             complement(3253..>4286)
                     /locus_tag="VKQ27_20925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91459.1"
                     /translation="GSLFWGSLSDRFGTRTVVLIGGALLGAGVIATSYATSLLQFQLL
                     FGVAVGLSVGAFYAPLTASVTRWFTAHRSLAVALVSAGLAMGSLLIAPLSRWIITQYG
                     WREAMLTLGCLAWLVILPAALLLRRPPTLAHAAAAGAGGQDFTIWQVLRTPQFAAIAL
                     THFTCCAAHSGPIFHMVSYAIDCGVPSMIAATVFSTAALAALSGRIACGVLADRLGAK
                     RTLVAGLALQALAVSLYVFSRDTASFYALSLLFGIAYGGVMPLYAILVREYFGARVMG
                     TAFGTVAMISTLGMAIGPWAGGRIYDVLGSYFWLYIGSFGIGLGAVAIALTFRPPETR
                     PLALAQSLA"
BASE COUNT          789 a         1371 c         1474 g          652 t
ORIGIN      
        1 gtccaggtcg ccggcgatgc aagattcatt gaacacatcc ataacgcgat cggacagacg
       61 gcggcgaaga gcgcggttca tccgatcgcg gacgacctcg gacatgctga cctgtggcgg
      121 cggcagactc ggaacgtcgc gacgcgcgaa aaggtcgcca aagatattgt tcagtcggct
      181 cactgggcta ctccaggatg tgatgcggcg attctagttg acggtaccgt cgtccttgcg
      241 cgcttcgacc aggcgccagg tgaagccgcg cggcgcggca ggcagtggct cgccaggcgc
      301 cattgcgacc tgttcgcctg tcggcgcgcc aagcacgttg cgctgctcat agacaccacc
      361 cacgggcgcc cgagttccag gcggatactc cggcaccggc atgcacacaa ctccctttgc
      421 agcccaatct gcttccattc gcaacggatc attggacgtg gcctgtgtgc cgcatgctat
      481 ccgtacttct acgtatcccg agctcttcgc caggatcgca ggattaggtt acagcttcgt
      541 gctacaggga ccgggcgtca gaccggcagg gtaatcgtcg ctttgacacc gcccatcgga
      601 cggtttgcca aggtcacgtc gccgccgtgc gcgcggaaga tgttgcgcgc gatcggcagg
      661 ccgagtccga cgccgccagt ctcccggttg cgcgaaggtt cgccacgatg gaacggctcg
      721 aacacgcgct cgagctctcc aggagcgatt cctgggccgt cgtcctccac ttcaatgacc
      781 agcgtgccat cgattggagg caccatccgg atacgtgcac tcccgccata ggcgagcgca
      841 ttggaaacca ggttggtcag cgcgcgtttg agcgccagcg ggcgggcgcg catggtgaaa
      901 tggggcggtc cttcataagc aagcctggta gccagatcgg gccacgcatc gccagcctcg
      961 tcgagcacgg tgcgaagcag ttcggccaga tcgagcgggg ataccggttc cgtggtctgg
     1021 gcgtcgcggc cgaacgccag cgttgcggag accatcgcct caagctcctc aaggtcggac
     1081 agcatcttgt tgcgctgctc atcgtcctcg acgaactccg cccgtagttt gaggcgcgtg
     1141 atcggcgtgc gaagatcgtg accaatggcg gtgagcagct ctgtgcgatc ctgcacgaag
     1201 cggcggatgc gcgctgccat agtgttgaag gcggcggcgg cggtagcgac ctcggtgggg
     1261 ccatcctctg gcagcggcgg ggcgttcaca tcgcgaccca gcgcctcagc tgcggcggcg
     1321 agcgtgctga caggtgccgt cagccggcgg actgcccaca aggtgaggcc cgctgccgcc
     1381 agggtcatca ccaggaatgc agcgaggaag gtgggtgaat gccagggccg gggcggctca
     1441 taaggaacgg ttaccttgag ccattcgccc tcgggcggca tccgcatgcc aatgacgatg
     1501 atgtggtgcg acgcgtcagt caggatgcgc aagccgcgcc aatgctgcgc gcccagcagc
     1561 ggcacgaggt tcagattgac ccgcagcagc cgctggccga atgggacttc gtcaggcagg
     1621 tcctcggtgg gaggatcctc gtcgatttct gcggccagtc ccggttcccg gtggatctcg
     1681 gccagcaccg tctgacgccg gtcgggcgag gtcatcatca ctgtccggta gacgctgacg
     1741 acacggacgg cgatttcgcg cgcctgcgcc agacgctgca cgtcgatgcg gtcgagggca
     1801 tggatcgcca gccccgcgac ctgcaccacg gcgagcccaa ccagcagtac gacagcagtg
     1861 cgggccttga ggcttcgcgg ccagaggcga aggttcacac gcgttcgacc atcgccgcca
     1921 atacgtagcc cccaccacga accgtcttga tgagctgcgc gttgcgaccg tcatcctcca
     1981 gcttgcggcg caagcggctg atggcgacgt cgatcgcgcg gtcgaacggg ccggcctgcc
     2041 gcccgcgcag cagatcgagg agcatatccc tggttagcac gcgattggcc cgatcgacca
     2101 acgccaccag cagatcgtac tcgccgccgg tgagagcgac ctcggcgccg tcgggattca
     2161 gcaggcggcg gcgcccaggc tccagggtcc agccagcgaa tttcaggctg cgcgcgttcg
     2221 cgtcggggcg gtgatcggcg ccgtcgccga cccggcgcag cactgcccgg atacgggcga
     2281 gcaactcccg tggattgaag ggtttcggaa cataatcgtc ggcgccaagt tcgagaccga
     2341 tgatacgatc agtttcctca cccatcgcgg tgagcatgac gatgggcaca tcggactggg
     2401 tgcgcagcca gcgggccagg tcgaggcctc cctcgccggg cagcatcaga tcgagtacga
     2461 cgagatgata gtggccgttg gcaaaggcgc ggcgggcctc acgcccgtcg cgaacggccg
     2521 taacccgcat gtggtgcttc tcttccagga agcgggcgag caggtcgcgg atctcgcgat
     2581 cgtcgtcgac aaccagaatg tgcggcacat gttccatggg tcggaacata cgggtactcc
     2641 atccgcttcg gcagggtggc ttgcactatg tcgcacttcg tcgcgcctct gtcacaaacg
     2701 cgcaatcctc cgcccccagg ttgcgattta ccaggggaca caatgcccta tttggagaag
     2761 acgatgcgaa gaaccatcct agcaactgtt gccgtgctcg ccattggggg tgggacggct
     2821 ggcgtgctga tcgcccaggc gcagcccgcc ccttcgccgc agaccgccga cgctccgtcg
     2881 cccccgcaac gtccgcactg gatggggtgg atgcaccggg ggcgcgacgg gcacggcggc
     2941 ccctgggccg cgatgcggcg caattttgcg ctggtctacc ggcatgagga tcgccagctc
     3001 gcgccaccgg atgtgcagaa gatcgccgag gcgttcctgc tgtggaacgg taaccacacc
     3061 tggaaggtgg ccaacgtgaa gccggtatcg gacggcacca tcggatttga cctgacgacg
     3121 gcgagcggcg acgtgatcgc cagcttcacc atggacacgc acacggcacg ggtaacgcgg
     3181 gccggctgag ccgcgcggcg ccgggcgggc gcgatttccc cacacgcccg cccggcggag
     3241 gctacgtgac gttcaggcga ggctctgcgc caacgccagg ggacgcgttt ccggtgggcg
     3301 gaaggtaagc gcgatcgcca cggcgccgag gccgatgccg aacgagccga tgtagagcca
     3361 gaagtaactg ccgagcacat cgtagatacg gccaccggcc caagggccga tggccatgcc
     3421 cagcgtggag atcatggcga ccgtcccgaa tgcggtgccc atgacgcggg cgccgaaata
     3481 ttcgcgcacc aggatggcgt agagcggcat gacgccgcca taggcgatgc cgaacaacag
     3541 tgacagggca tagaagctcg cggtgtcgcg gctgaagacg tagaggctga cggccaacgc
     3601 ctgcagcgcc agaccggcaa ccagcgtgcg cttggcgccg aggcggtcgg cgagcacgcc
     3661 gcaggcgatg cgcccgctga gtgcggcgag ggcggcggtg ctgaacaccg tggcggcgat
     3721 catcgatggc acgccgcaat cgattgcgta gctgaccatg tggaaaatcg ggccggaatg
     3781 cgccgcgcaa caggtgaagt gcgtcagcgc gatggcggcg aactgcggtg tgcgcagcac
     3841 ttgccagatg gtgaagtcct ggccgccggc gccggccgcc gcggcgtgcg ccagggtggg
     3901 gggacggcgg agcagcagcg ccgcgggcaa gatcacgagc caggcgagac agccgagtgt
     3961 cagcatcgcc tcgcgccagc cgtactgcgt gatgatccag cgcgacagcg gcgcaatcag
     4021 cagggaaccc atcgccaggc cggcggagac cagtgccacg gcaaggctgc gatgtgccgt
     4081 gaaccagcgc gtgacgctgg cggtgagcgg ggcatagaag gcgccgaccg acaggccgac
     4141 ggcgacgcca aacagcaact ggaattgcag cagcgaggtc gcgtagctgg tcgcgatgac
     4201 gccggcgccg agcagggcac cgccgataag gacgacagtg cgggtgccga acctatcgga
     4261 cagcgaaccc cagaacagcg atccgg
//
LOCUS       DATGEM010000677         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_175097, whole genome shotgun sequence.
ACCESSION   DATGEM010000677 DATGEM010000000
VERSION     DATGEM010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175097"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1701)
                     /locus_tag="VKQ27_20930"
     CDS             complement(109..1701)
                     /locus_tag="VKQ27_20930"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLY91460.1"
                     /translation="MDADYIVVGAGSAGCVIASRLSETGASVTLLEAGPRDWHPMIHI
                     PAGVLKLLYHPVVNWNYASAPETGTAGRSIHWPRGKVLGGSSSINGMLYVRGNPADFD
                     GWAQMGCRGWSFDDVLPFFRKSEHYVQGGDREIRGQGGPLKVEDYRTILPLTHRFVEA
                     AQQAGFPFSADLNGRMQEGVGYSQMTRKGRFRGSTAQTFLSEARKRDNLRIETDALAT
                     RLLLDGKRCVGVAFRQRGMDREARAAREVILCGGSVNSPHLLQISGIGPAQHLQSIGV
                     QVVHDLPGVGANLQDHYVARISHRAKDATSINQLAHGVRLAAEAVRFAAVGRGALTFG
                     VTSAQVFCRSRPELASPDLQLLFTPASYDAARFGALEREAGMTVAICPVRPDSRGTIM
                     AQSPDPSAAPLITPNYLSDPNDQRVLTTGMRITRTIFAAPAIARHSVRETLPGPGVMS
                     DDELADYSRRFGTTIFHPVGTCRMGDGPAAVVDARLRVHGMGGLRVIDASVMPTLTTG
                     NTNAPTIMIAEKGAAMIREDAA"
     gene            complement(1701..2285)
                     /locus_tag="VKQ27_20935"
     CDS             complement(1701..2285)
                     /locus_tag="VKQ27_20935"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLY91461.1"
                     /translation="MHFEGEFSVPGKPADVIQRFADVERMTRCMPGAVIDGRDEEGNY
                     LGGMLVSFGPKKIKFKGRVTVENDPASLTGSLQGRGAADMRAARIAVRISYALRDDPE
                     KAGATLVKLTSDAELGGVLADFARTGGVPFANALMLDFSRRVAEEFANEDATPAVGTA
                     AATEPVPLAAHGLLWQVIKAKLLEFARWLGLARA"
     gene            complement(2289..>3965)
                     /locus_tag="VKQ27_20940"
     CDS             complement(2289..>3965)
                     /locus_tag="VKQ27_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY91462.1"
                     /translation="DWDYDYRPSTQFLPTRMETPSQDQLAPVIEKLLAAERPVIIAGR
                     GARAANAKDEIVKLADRVGALLATSLQGKGYFAGHPWDIGIAGAFASAPSEQLLAEAD
                     FVLGVGAELGYYTTEGGLLFPSAEVARIDIKAMPEEIAEAFYAARQYRGPVVLNLPMD
                     LQEDDFDWDYDYRPSTQFLPTRMETPSSDQLAPVIEKLLAAERPVIIAGRGARAANAK
                     DEIVKLADRLGALLATSLQGKGYFAGHPWDIGIAGAFASAPSGQLLAEADFVLGVGAE
                     LGYYTTEGGLLFPSAEVARIDIKAMPEEIGVIPGLYVQADGRKAVAAINEALESRQVR
                     KEGMRTASTRAVLDAPDHRFDAPKDGLDPRALARNLGAALPKGALFTVGAGHFFTWIG
                     MYTPLPEGVEIQYSYGFGAVGQGIGVAIGTGAGNPKRQHIAVEGDGSLMFNIQELETI
                     VRHKMQMVLIVWNDEGYGAEVHKLVAKGFDEKLAQWESPNFVALAKAFGGDGVLLKDP
                     SEIGGAVAEGLRKGGLYLIDARVSPTTPTEPYAKVHFGRESHAPLLRPLN"
BASE COUNT          685 a         1376 c         1187 g          717 t
ORIGIN      
        1 gcaacatggc tatgcgccgg ggctgttgac tgcgatggca gcagctgaag aggtggcgca
       61 gtattggcat gcacttggcg ggactgctgc cggtatctga tttattcttt acgcagcatc
      121 ctcccggatc attgccgcgc ccttctccgc gatcatgata gttggcgcgt tcgtattgcc
      181 cgtggtcagc gtcggcatga ccgaggcatc gatcacgcgt agcccgccca tgccatgcac
      241 gcgcagcctg gcgtcgacga cagccgccgg tccatccccc atccggcagg ttcccaccgg
      301 atggaatatc gtcgtgccga accgccgcga ataatccgcc agttcatcgt ccgacatcac
      361 acctgggccc ggcagtgtct ccctgacgct atgccgcgcg atcgcaggtg cggcgaagat
      421 cgtgcgtgtg atccgcatac ctgtcgtcag tacgcgctgg tcatttggat ccgacagata
      481 gttcggggtg atcagcggcg cggcggatgg gtcgggtgac tgcgccatga tcgtgccccg
      541 gctatcgggt cgcaccggac atatcgccac tgtcatgccg gcctcccgct ccaaggcgcc
      601 gaagcgcgcc gcgtcgtagc tcgccggcgt gaagagaagt tgcaggtcag gactggcaag
      661 ctctggtcgc gaccggcaga acacctgagc gctggtgacc ccgaacgtca gcgcgccgcg
      721 gccgacggca gcgaagcgca ccgcctctgc ggccagccgc acaccgtgcg cgagctggtt
      781 gatcgaggtc gcgtccttcg ctcgatgcga aatccgcgcg acgtaatggt cctgtaggtt
      841 ggctccgacg cccggcaggt catgcacgac ctggacaccg atcgattgca gatgctgcgc
      901 cggcccgatg ccggaaatct gcagcagatg cggcgaattc accgagccgc cgcacaggat
      961 gacctcgcgc gccgcccgcg cctcgcggtc catgccacgc tgccggaatg cgacaccaac
     1021 acaccgcttg ccatccagca gcaggcgcgt cgccagcgcg tcggtctcga tccgcaggtt
     1081 gtcgcgcttg cgtgcctcgg acaggaaggt ctgcgcggtc gaaccgcgga accgcccctt
     1141 ccttgtcatc tgcgaatagc caacgccttc ctgcatccgt ccattcagat cggcgctgaa
     1201 tggaaatccc gcctgctgcg ccgcctccac gaagcgatgc gtcagcggca ggatggtgcg
     1261 gtagtcctca accttcagcg gaccaccctg gccgcggatt tcccgatcgc cgccctgcac
     1321 gtagtgttcc gatttgcgga agaacggcag cacgtcatca aaactccagc cgcgacagcc
     1381 catctgggcc caaccgtcga aatccgccgg attgccgcgc acatacagca tgccgttgat
     1441 cgagctcgat ccgcccagca ccttgccgcg cggccaatgg atcgaccgcc ccgccgttcc
     1501 tgtctccggc gccgaggcat agttccagtt caccaccgga tgatacagca gcttcagtac
     1561 gccggcgggg atatggatca tcggatgcca gtcgcgcggt cccgcttcca gcagcgtcac
     1621 gctcgcgccg gtttcgctga gccggctggc gatcacacag cccgcggagc cggcgccgac
     1681 gacgatgtag tccgcgtcca tcaggccctc gcgagcccaa gccagcgcgc gaactccaac
     1741 agcttcgcct tgattacctg ccacaacagg ccatgtgccg cgagcggcac cggctctgtt
     1801 gccgcggccg tgcccactgc aggtgtcgcg tcctcattgg cgaattcctc agcgacacgc
     1861 ctggaaaagt ccagcatcag cgcattggcg aacggcacgc cccccgtgcg cgcgaaatcc
     1921 gccagcacgc caccaagttc cgcatccgac gtcagcttca ccagcgtcgc cccagccttc
     1981 tccggatcgt cgcgtaaggc gtaactgatg cgcactgcta tccgtgcagc gcgcatgtcc
     2041 gcggcaccac ggccctgcaa cgagccggtc aaggatgccg ggtcattctc gaccgtgacc
     2101 cggcccttga acttgatctt ctttggcccg aacgacacca gcatgccgcc gaggtagttg
     2161 ccttcctcgt cgcgaccatc gatcaccgcg ccaggcatgc atcgcgtcat gcgctctacg
     2221 tcggcgaagc gttggatcac atccgccggc ttgccgggga cgctgaattc gccctcgaag
     2281 tgcatcgctc agttcaacgg ccgcagcaga ggcgcgtggc tctcgcggcc gaaatgcact
     2341 ttcgcgtagg gttcggtcgg tgtcgttggc gatacgcgcg catcgatcag gtagagacca
     2401 cctttgcgca ggccctcggc gacggcgcca ccgatttccg acggatcctt gagcagcacg
     2461 ccgtcgccgc cgaatgcctt ggccagagca acgaagttcg gcgattccca ctgcgccagt
     2521 ttctcatcga agcccttggc caccagcttg tggacctcgg cgccatagcc ttcgtcgttc
     2581 cagacgatca gcaccatttg catcttgtgg cggacaatcg tttccagctc ctggatgttg
     2641 aacatcaggc tgccatcgcc ttccaccgcg atgtgctgcc gtttcggatt gcccgcaccc
     2701 gtgccgatgg cgacgccaat cccctgaccg accgcgccaa agccgtacga atactggatc
     2761 tccacgcctt ccggcagcgg cgtatacatg ccgatccagg tgaagaaatg cccggcgccg
     2821 acagtgaata gggccccctt gggcaacgcc gcacccaggt tccgcgccag tgcccgcgga
     2881 tccagtccat ccttgggtgc gtcaaacctg tggtctggcg cgtccagcac cgcacgcgtc
     2941 gaggcggtgc gcatgccttc cttgcggacc tggcgcgatt ccagcgcctc gttgatcgca
     3001 gcgacggcct tacgtccatc agcctgcaca tacagtccgg gaatgacgcc gatctcttcc
     3061 ggcatcgcct tgatgtcgat gcgcgcgacc tcggccgacg ggaacagcag gccgccttcc
     3121 gtcgtgtagt agccaagctc cgcgcctacg cccagcacga agtccgcttc cgccagaagc
     3181 tgtcccgatg gcgccgatgc gaatgcgcct gcgatgccga tatcccacgg atggccggcg
     3241 aaatagccct tgccctgtag tgacgtggca agcaatgcgc cgaggcgatc cgccagcttg
     3301 acgatttcgt ccttggcgtt ggccgctcgc gcaccacggc cggcgatgat caccggtcgt
     3361 tcggcagcca acagcttttc gatcaccggc gccaactggt cggagctcgg ggtctccatg
     3421 cgcgtcggca ggaactgcgt cgatgggcga tagtcatagt cccaatcgaa atcatcttcc
     3481 tgcagatcca tcggcaggtt cagcaccacc ggcccgcggt actgccgcgc ggcatagaat
     3541 gcttcggcga tctcttccgg catcgccttg atgtcgatgc gcgcgacttc ggcagagggg
     3601 aacagcaggc cgccttctgt cgtgtagtaa ccgagctccg cgccgacgcc gagcacgaag
     3661 tcggcttccg ccagaagctg ttccgatggc gccgatgcga aggcaccggc gatgccgatg
     3721 tcccacgggt ggccggcgaa atagcccttg ccttgtagtg acgtggcaag caatgcgccg
     3781 acgcgatccg ccagcttgac gatttcgtcc ttggcattgg ccgcgcgggc gccccggccc
     3841 gcgatgatca ccggccgttc ggcagccagc agcttttcaa tcaccggcgc caattggtcc
     3901 tgactgggtg tctccatgcg cgtcggcaag aactgcgtcg aggggcgata gtcatagtcc
     3961 cagtc
//
LOCUS       DATGEM010000678         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_230829, whole genome shotgun sequence.
ACCESSION   DATGEM010000678 DATGEM010000000
VERSION     DATGEM010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230829"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VKQ27_20945"
     CDS             complement(<1..478)
                     /locus_tag="VKQ27_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91463.1"
                     /translation="MAIIVGSGDYRYEQIDNWAKVPPGWSLREVGAVGVDAQDNVYVF
                     NRGEHPMMVFDREGNFLRAWGEGLFPRAHGLHMAPDGTVFLTDDGDHSVRQCTLNGKV
                     LLTLGTPGKPAPFMGGEPFHRCTHTALSPKGEIYVSDGYGNARVHKYAPDGRYLMSW"
     gene            576..1805
                     /locus_tag="VKQ27_20950"
     CDS             576..1805
                     /locus_tag="VKQ27_20950"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLY91464.1"
                     /translation="MSRRNTVQLLGSALAAPALLRTAWADDKVLSIGMTFPITGTLAL
                     QAGVVRDAVLYAINEANGKGGIAGYKLRDVTLDDASPTTGQYDPALAATNARKLLQDP
                     AIIAAVGPLNSGSAKAVSPIYSQGGMATISGSATNPDLTDPKFAQLYHPASPPIFFRT
                     VTTDAYQGPNLANYFAETLKIQKIVVLDDGGAYGVGMANAFAAQAAKKGMTVLIRDRL
                     DPLNADYTAVLTRAKSLDTQALYYGGDPGAGAKVVKQSYDILPKIAKGGGDGVHTPDM
                     LTGGGMPAIEGWYATSAAAHVLDQTDIQPWAKDYLAKMGKQPADYTVTYYDAAQVIFD
                     AIARVAKSGKTVNRANVRDAIATTNLKTLQGDISFDANGDMNSKIVSIYQVKYDSNHP
                     IGDVVHQFRYVGVAPEA"
     gene            1802..2857
                     /locus_tag="VKQ27_20955"
     CDS             1802..2857
                     /locus_tag="VKQ27_20955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY91465.1"
                     /translation="MKFSNFLFPESRDPAHDGQVIDETLREAQLTDELGFDVIWLAEH
                     HFDGICAYVDPVSFAASLAVATHRAKIGFAVAQMSLHHPIRMAEQLALIDNISKGRLI
                     VGLGRGTAYNIYDYQGYGLDHHEAQARFEEAEAIMFAAWRGDKFEHHGRFWDLKLPML
                     RPRPYTQPHPYVIRSASSEEGMLHIARQGRPFMMNVQSNEVTQHRMDLYRRTLREQGM
                     DEATVASTVAQCWVWRNVFVAETDAEAARIAIPAFEAMHTFRAAMRQRVYEEQGVSIV
                     PMPASGTAPAARTMIEHSLIYGSPATVAEALAKVDAIGVGGVIMQFRLGPMAYDDVAQ
                     SLTLFQQKVAPELQRRG"
     gene            complement(2808..4457)
                     /locus_tag="VKQ27_20960"
     CDS             complement(2808..4457)
                     /locus_tag="VKQ27_20960"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY91466.1"
                     /translation="MRRLVTRVLVVGGGPVGTTLAIDLAWRGIDVFVAEQRHRGEPPS
                     VKCNHVAARTMETFRRLGIARQVRDAGLPPDHANDVAFRTTTTGIEFARIKIPCRRDR
                     FSVTDGPDGWWPTPEPPHRINQLFLEPALVECARAMPRLIYMNRLKVLGFSQTDHGVV
                     AEAQDLDSGETLLIACEYLIGCDGPSSEVRRQIGARFSGDAMIGRTQSTYIRAPSLAR
                     MMQAEPAWSTQSLNPRRSANMFAVDGRETWLIHNYLRPDETFDSVDRDRCIRMILGVS
                     PDFDYQILTQEDWIGRRMIADRFRDRRIFICGDAAHIWVPFAGYGMNAGIADAMNLSW
                     MLAGVLQGWAAPAILEAHEAERWPITEQVSHYAMNTAQSLARARAEVPQNIETDAEVR
                     ERFGRAVRDLNVPQYCCGGLNFGYFYDRSPIIAYDGETAPPYTMYDYTPSTVPGCRTP
                     HVWLHNGSSLYDLMECGFSLLRFDRSVAVDKLVGAATKRGVPLAVLDIDAASPYEHAL
                     VLSRPDQHVAWRRNQLPDDPLKLIDTIRGAAIPARPSAGTT"
     gene            complement(4457..>5375)
                     /locus_tag="VKQ27_20965"
     CDS             complement(4457..>5375)
                     /locus_tag="VKQ27_20965"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLY91467.1"
                     /translation="FALSANHPMFQGSMPGPLLADADLIVVIESDVPWLPSREEPPPG
                     ARIVQIGEDPLYARYPMRSFPSDLTITAATVSVLEALEQALQGRKGVHVDDRRARLVA
                     RSTELHKQWNDEAAQAGRGNKNTLAWLNHCLRDVVDRDTMVISEYSFRQEYCPLETPG
                     SLYAVSSAGGLGWGFGASLGAKLAAPDRMVLSVLGDGAYMFANPTACHYIAQMQKLPV
                     LTIIYNNALYGAVRRATLDMYGAGTAAEADGRLLADLPAPNFEKIVSAHDGHGERVER
                     PADLPAALKRSADAVRGGQQALVNVICPP"
BASE COUNT         1046 a         1711 c         1638 g          980 t
ORIGIN      
        1 cccatgacat caggtaacgc ccgtccggcg cgtatttgtg cacacgtgcg ttgccgtagc
       61 cgtccgaaac atagatctcg cccttcggcg acaatgccgt atgcgtgcag cgatggaacg
      121 gctcgccacc catgaacggt gccggcttgc cgggggtgcc aagggtaagc agcaccttgc
      181 cattcagcgt gcactgccgc accgagtgat cgccatcatc ggtgaggaac accgtgccat
      241 cgggcgccat gtgcaggcca tgcgcgcgag gaaacagtcc ttctccccat gcgcgcagga
      301 aattgccctc gcgatcgaac accatcatcg gatgttcgcc gcgattgaac acatagacgt
      361 tgtcctgcgc atcgacacca accgcgccga cctcgcgcag cgaccaacct ggcggcactt
      421 ttgcccaatt gtcgatctgc tcgtagcggt agtcaccgct gccaacgatg atggccatgg
      481 cgtttcctcg gctgttatcc tgtccgcccc acatgacaag cgtttcgttc tgtaccatgc
      541 tgccgaaagc ttggaaagga tagcggatgc ctcgtttgtc tcgacgcaac accgtccagt
      601 tgctgggttc ggcactggcc gcaccggcct tgctgcgaac cgcgtgggcg gacgacaagg
      661 tgctgagcat cggcatgaca tttccgatca ccggcacgct ggccttgcaa gccggcgtcg
      721 tgcgcgacgc cgtcctgtac gccatcaacg aagcgaacgg gaaaggtggc atcgcgggtt
      781 acaagctccg cgacgtgaca ctcgacgacg cttcgccgac cactggccag tacgaccccg
      841 cacttgccgc aacgaatgca cgcaagctgt tgcaggaccc ggcgattatc gccgcggttg
      901 gacctttgaa cagcggttcc gccaaggcgg tcagtcctat ttacagccag ggtggtatgg
      961 caacgatcag cggcagcgcc accaatcctg atctcaccga tccaaagttc gcccagctct
     1021 accatcccgc gagcccgccg atcttcttcc gcacggtcac gaccgatgcc taccaggggc
     1081 ccaatctggc gaattacttt gccgagacgc tgaagatcca gaagattgtc gtgctggatg
     1141 atggcggtgc ctacggcgtc gggatggcca atgccttcgc cgcgcaggcc gccaagaagg
     1201 gcatgactgt gctgatccgt gataggctcg atccgttgaa tgccgactat accgccgtcc
     1261 tgacgcgggc gaagagcctg gacacccagg cactgtacta tggcggcgat ccgggtgccg
     1321 gcgcgaaagt ggtgaagcag tcctacgaca tcctgccgaa gatcgcgaag ggcggcggcg
     1381 acggcgtgca cacgcccgac atgctgacag gcggaggcat gccggcgatc gaagggtggt
     1441 acgccacatc ggctgccgcg cacgtgctgg accagaccga catccagccc tgggccaagg
     1501 attacctcgc caaaatgggc aaacagcccg ccgactacac cgtcacctac tacgatgcgg
     1561 cacaggtgat cttcgatgcc atcgcgcgcg tcgcgaaatc cggcaagacc gtgaaccgtg
     1621 cgaatgttcg cgatgcgatc gcgacgacga acctcaagac gctgcaaggc gatatttcgt
     1681 tcgacgccaa cggcgacatg aactccaaga tcgtgtcgat ctaccaggtg aaatacgaca
     1741 gcaaccatcc gatcggggac gtcgtgcatc aattcagata tgtcggtgtg gcgcccgagg
     1801 catgaagttc agcaatttcc tgtttcctga gagccgtgat cccgcgcacg acggccaggt
     1861 gatcgacgag acgttgcgcg aggcgcaact caccgacgag ctcggcttcg acgtcatatg
     1921 gctcgccgag caccacttcg acggcatttg cgcctatgtc gatccggtga gtttcgctgc
     1981 gtccctcgcg gtggcaacgc accgtgccaa gatcggtttt gccgtggcgc agatgtcgct
     2041 acatcacccg atccgcatgg ccgaacagct cgcactgatc gacaacatca gcaaggggcg
     2101 gctgatcgtc ggtctcggcc gtggcaccgc ttacaacatc tacgactacc agggctatgg
     2161 tctcgaccat cacgaggcgc aggcgcgctt cgaggaagcc gaggccatca tgttcgccgc
     2221 ctggcgcggc gacaaattcg agcatcatgg ccgcttctgg gacctgaagc tgccgatgct
     2281 acggcctcgt ccgtatacac agccgcatcc ttacgtcatc cgctctgcct ccagcgagga
     2341 gggcatgctg cacatcgccc gtcaggggcg gccgttcatg atgaacgtgc agtccaacga
     2401 ggtgacgcag caccgtatgg acctgtatcg ccgcacgctg cgcgagcagg gcatggacga
     2461 agcaacagtc gccagcaccg ttgctcagtg ctgggtctgg cgcaacgtat tcgtggccga
     2521 gaccgacgcc gaagctgcgc gcattgccat tccagctttc gaagcgatgc acacgttccg
     2581 tgccgcgatg cgccagcgcg tctatgagga acaaggtgtg tcgatcgtgc cgatgcctgc
     2641 atctggaacc gcgcccgccg cgcgcacgat gatcgagcat tcgctgatct acggctcgcc
     2701 tgcgaccgtc gcggaagcgc tggcgaaagt ggatgccatc ggcgtcggtg gcgtcatcat
     2761 gcagttccgg ctgggaccga tggcttatga tgacgtggcg cagagcctca cgttgttcca
     2821 gcagaaggtc gcgccggaat tgcagcgccg cggatagtgt cgatcagctt gagcggatcg
     2881 tccggcagct ggttacgccg ccatgcgacg tgctgatcgg ggcgggacag cacgagtgca
     2941 tgttcatagg gtgatgccgc gtcgatatcg agaacggcaa gcggtacacc gcgcttcgtc
     3001 gcggccccga ccagcttgtc cacagcaacg gaccgatcga accgcaacaa ggagaaacca
     3061 cactccatta ggtcgtagag cgaactgccg ttgtgcagcc agacatgcgg cgtacggcag
     3121 ccaggtacgg tggacggggt gtaatcgtac atcgtgtaag gcggcgcggt ttcgccatca
     3181 tatgcaatga tcggcgaacg atcgtagaaa tagccgaagt tcaggccacc gcagcagtat
     3241 tgcgggacat tcagatcacg cacggcgcga ccgaagcgtt cgcgcacctc cgcatccgtc
     3301 tcgatattct gcggtacttc ggcgcgtgct ctcgccagtg attgcgcggt gttcatcgca
     3361 taatgcgaca cctgttcggt gatcggccat cgctccgctt cgtgcgcctc caggatcgcc
     3421 ggtgcggccc agccttgcag cacgccggcc agcatccacg acaggttcat ggcatcggca
     3481 atgccggcat tcatgccata accggcaaat ggcacccaga tatgcgccgc gtcaccacag
     3541 atgaagatac gccgatcgcg gaatctgtcg gcgatcatgc gccgaccgat ccaatcctcc
     3601 tgcgtgagga tctgataatc gaagtctgga cttacaccca ggatcattct gatgcagcgg
     3661 tctcgatcga cgctgtcgaa ggtctcgtca gggcgcaggt agttgtggat cagccaggtc
     3721 tcgcgcccat cgaccgcgaa catgttggcg ctgcggcgag gattcagcga ttgcgtggac
     3781 caggctggtt cggcctgcat cattcgggca agcgacggcg cacggatata cgtcgattgc
     3841 gttcgcccga tcatcgcgtc gccggagaat cgcgcaccga tctgccggcg gacctccgac
     3901 gacggtccgt cgcagccgat gagatactca caggcaatca atagtgtctc gccggagtcc
     3961 aggtcctgtg cttcggctac cacgccgtga tcggtctggg agaatccgag cactttcagc
     4021 cggttcatgt agatcagccg cggcatcgcg cgggcgcact ccaccagtgc cggttcgaga
     4081 aacagctgat tgatccgatg cggcggctcc ggtgtcggcc accagccgtc tggcccgtct
     4141 gttacgctga agcggtcgcg ccggcagggg atcttgatgc gggcgaactc gatgccggtg
     4201 gtcgtggtac gaaaggccac atcgttggca tgatcaggcg gcaggcccgc atcgcgcacc
     4261 tgccgcgcga tgcccagccg gcggaaggtc tccatggtgc gcgccgcaac gtggttgcat
     4321 ttgacgctcg ggggttcgcc tcggtggcgc tgttccgcca cgaacacatc gatgccgcgc
     4381 cacgccaggt ctatcgcgag cgtcgtgcct accgggccgc cgccaacgac cagcacacgc
     4441 gtgacaaggc gccgcatcag ggagggcaga tcacgttcac cagcgcctgt tgcccgccgc
     4501 ggaccgcgtc agccgatcgt ttcaatgcgg cagggagatc agctggacgt tccacccgct
     4561 cgccatgacc atcatgcgcc gaaacgatct tttcgaagtt gggtgccggc agatcggcca
     4621 gcagccggcc atctgcttcg gccgccgtgc ccgcgccgta catgtccagc gtggcccgcc
     4681 gcaccgcgcc atacagcgca ttgttgtaga tgatggtcag caccggcagt ttctgcatct
     4741 gcgcgatgta gtgacacgcc gtcggattgg cgaacatata ggcgccatcg ccaagtaccg
     4801 acagcaccat ccgatctggc gctgccagct tggcgccgag cgaggcaccg aagccccatc
     4861 ccagtccgcc agccgaactc accgcataca gactgccagg cgtctccaat ggacagtatt
     4921 cctggcggaa tgaatattcg ctgatcacca ttgtgtcgcg atccacgacg tcgcgcaggc
     4981 agtgattcag ccaggccagc gtgttcttat taccgcgtcc ggcctgcgct gcttcgtcgt
     5041 tccattgctt gtgcagttcc gtggatcgag ccaccagccg agcgcgacga tcgtccacgt
     5101 gcacaccttt gcggccttgg agcgcctgct ccaatgcttc caggaccgac acggtggcag
     5161 cggtgattgt caggtcggaa gggaagcttc gcatcggata gcgggcgtag agcggatcct
     5221 cgccgatctg cacaatacgc gcaccgggtg gaggttcctc gcggctaggc agccacggca
     5281 catcggattc gatcaccacg atcagatcgg cgtcggccag cagtgggccc ggcatcgatc
     5341 cctggaacat cgggtgattg gcggaaagcg caaaa
//
LOCUS       DATGEM010000679         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_955035, whole genome shotgun sequence.
ACCESSION   DATGEM010000679 DATGEM010000000
VERSION     DATGEM010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955035"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..213
                     /locus_tag="VKQ27_20970"
     CDS             67..213
                     /locus_tag="VKQ27_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91468.1"
                     /translation="MQGRIFWATWIILDLIAGLLLPFWWALVASIPTGILAWWVAYKS
                     DWFG"
     gene            410..616
                     /locus_tag="VKQ27_20975"
     CDS             410..616
                     /locus_tag="VKQ27_20975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLY91469.1"
                     /translation="MCAALPNIELHPRQKLFVGFRFEGHAELIKVRVMVRHHEGDRYG
                     FQFLDITPDQRNQIRMACQGLQII"
     gene            855..>2567
                     /locus_tag="VKQ27_20980"
     CDS             855..>2567
                     /locus_tag="VKQ27_20980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLY91470.1"
                     /translation="MHKPVKYVEKAVTAAAAGVWAVFERLNRIKPNPSFTPKWSDKPL
                     LKSWQKTKPPLGWPRATDSLCPRCVPEIRQQILDGKLPHEILLNEKVGEIKAQIIERD
                     GKILMVKDCPKHGHFEDVMAIDTDFFKHLESVFPGRDILAHNDEKLHNHGTSTVKHGR
                     GSVLTVDLTNRCNMMCDPCFMDANQVGFVHELTWEEIKQVLDNAISIKPRRQMSVQFS
                     GGEPTLSPYFLDAVRYSRKVGYNSVQAATNGIEFAKSKEFCKQAAEAGLRYAYLQFDG
                     IGNAANSHRAVGNLFDVKLQAIQNLHEAGVDIVPVTTIVNGINNEQVGRIIQFALDNP
                     KRISFLSFQPVSFTGRDEEITDERRQAQRYTLSHLAHDVKSQTGLGEPSRDWFPISFM
                     STFSDWADLIHGPAAEWGQLSCGCHPNCGVGMAVMIDKETKEAVPVTAFLNGDRLARD
                     IAKVNDAARGRFLSVVGMALALMRNYDSFQSPKHFKMVDLLRKFDKTFAASKNADKKY
                     GSVKGERTEEDIKRRRNDRWNFLFIAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFC
                     AYNTG"
BASE COUNT          519 a          809 c          764 g          475 t
ORIGIN      
        1 aagccgtaag gccacgtctc gttgacggac cggccgcggg cgtaaacacc ctgatacagt
       61 gggcgcatgc agggccgcat cttctgggcg acgtggatca tcctcgacct gatcgcgggg
      121 ctgttattgc cgttctggtg ggcgctcgtc gccagcatcc ccaccggcat cctcgcctgg
      181 tgggtggcgt acaaaagcga ttggttcgga tagtcattgc accgcaatcg gggctgccgt
      241 aggatgcagc gcagccgtat atgtctccca accagctgga accggaacgc caatggcgta
      301 tccaacgtag ttttccgcgc ttcgcgctgg agctgccggt gtccttcgcc gagacgcgcg
      361 agggcgagcc cacgatcatg ggcacaatgg tggacgtcgg gctggtggtt tgtgtgccgc
      421 gctgccgaat atcgaattgc acccgcggca gaaattgttc gtcggctttc gcttcgaagg
      481 ccacgccgag ctgatcaagg tgcgcgtcat ggtccgtcac cacgagggcg accgctacgg
      541 ctttcagttc ctcgacatca cccccgacca gcgcaaccag atccgcatgg cgtgccaggg
      601 gctgcaaatc atctgagctg cccgtatccc attgattctt ttgcccgaaa tggcattttc
      661 gcacttgcat ttcactgcaa aatgtcgcca ttgaacacca ttaatccgcc attcacagct
      721 tcgcggttgg ggtatcccct gtccggccaa taacctagcg gaagaatcca cagggccctg
      781 gggtggtcgt gtatcggcct tgccttaatc cctaagacga gtttgctggg tgatagcgcc
      841 caaggagcga acggatgcat aagccggtca agtacgttga gaaagcggtc acggcagcgg
      901 cagcgggtgt gtgggcggtg ttcgagcgcc tcaaccgcat caagccgaac ccgagcttca
      961 caccgaagtg gtcggacaag ccgctgctga agtcgtggca gaagaccaag ccgccgctcg
     1021 gctggccgcg cgccaccgat tcgctttgcc cgcgctgcgt gccggagatc cgccagcaga
     1081 tcctcgacgg caagctgccg cacgagatcc tgctcaacga gaaggtcggc gagatcaagg
     1141 cgcagatcat cgagcgcgac ggcaagatcc tgatggtgaa ggattgcccg aagcatggcc
     1201 acttcgaaga cgtgatggcc atcgacaccg acttcttcaa gcaccttgaa tccgtttttc
     1261 ccggccgcga catcctcgcc cataacgatg agaagctgca caaccacggc acctcgaccg
     1321 tcaagcacgg ccgcggctcg gtgctgaccg tcgacctcac caaccgctgc aacatgatgt
     1381 gcgatccgtg cttcatggac gccaaccagg tcggcttcgt gcatgagctg acgtgggaag
     1441 agatcaagca ggtgctcgac aacgccatct ccatcaagcc gcgccggcag atgtcggtac
     1501 agttctccgg cggcgagcct acgctctcgc cgtacttcct tgacgcggtg cgttactcgc
     1561 gcaaggtcgg ctataactcg gtgcaggcgg cgaccaatgg catcgagttc gccaagagca
     1621 aggaattctg caagcaggcg gcggaagccg gcctgcgcta tgcgtatctg cagttcgacg
     1681 gcatcggcaa cgcggcgaac tcgcaccgcg ccgtcggcaa cctgttcgac gtgaagctgc
     1741 aggctatcca gaacctgcat gaagccggcg tcgacatcgt gcccgtcacg accatcgtga
     1801 acggcatcaa caatgagcag gtcggccgca tcatccagtt cgcgctcgat aacccgaagc
     1861 gcatcagctt cctgtcgttc cagccggtct cgttcaccgg ccgcgacgaa gagatcaccg
     1921 acgagcggcg ccaggcgcag cgctatacgc tgtcgcacct cgcgcacgac gtgaagagcc
     1981 agaccggcct gggcgagccc agccgcgatt ggttccccat ctcgttcatg tccaccttct
     2041 ccgattgggc cgacctgatc cacgggccgg cggcggagtg gggacaactt tcctgcggct
     2101 gccacccgaa ttgcggcgtc ggcatggccg tgatgatcga caaggaaacg aaagaagcgg
     2161 tgccggtcac ggcgttcctc aacggcgacc gccttgcgcg cgatatcgct aaagtgaacg
     2221 acgcggcgcg cgggcgcttc ctctccgtcg tcggcatggc gctggcgctc atgcgcaact
     2281 acgattcgtt ccagtcgccc aagcacttca agatggtcga cctgctgcgc aagttcgata
     2341 agacgttcgc cgccagcaag aacgccgaca agaagtacgg ttcggtgaaa ggcgagcgta
     2401 cggaagaaga catcaagcgg cgccgcaacg accgctggaa cttcctgttc attgccggta
     2461 tgtggttcca ggatttgttc aattacgact tccgccgcac ggagcagtgc atcattcctt
     2521 acgccaccca agaaggcgaa atctcattct gcgcctacaa tacgggc
//
LOCUS       DATGEM010000680         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1077881, whole genome shotgun sequence.
ACCESSION   DATGEM010000680 DATGEM010000000
VERSION     DATGEM010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077881"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1015
                     /locus_tag="VKQ27_20985"
     CDS             32..1015
                     /locus_tag="VKQ27_20985"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLY91471.1"
                     /translation="MAGIVRIRHTRVGVVRIGAFQPQAMPALCTAALAALAIPPDAPC
                     DDACQDRILIWAHDRMTHTLADRLEALRKAGARVLMVDISDNGGGTEWAEAAARMVSP
                     VTIRSEALGFVRGAHWETRWRNHADGLREAAAQAAPEDRARLLDWAAEADAARALAAT
                     PCAARSICIGRAGFATGLVSEAPAASFAGKPWADLVFSPAQYPYRDSVWRGPLIVLVD
                     DDTGSAAEEFAAVLQDNRAAIILGQRTGGAGCGHTDGGTPTALSNSGAVLELPDCIRF
                     RADGSNEVDGVIPDLPVAMRASDGPAREAQLVEPWLAQAVSRARRLRSTPR"
     gene            1115..1759
                     /locus_tag="VKQ27_20990"
     CDS             1115..1759
                     /locus_tag="VKQ27_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HLY91472.1"
                     /translation="MPLFRPALAFALASASLITPAPVAAKPAPETPLSPDLAQVQTHL
                     RSVATMTAGFVQTNRDGATVAGTLQLKRPGRVRFQYQEGVPLLVVGDGKALIMVDYKV
                     KQVSRWPVGNTPLGLLLNPEKDVGRYARIVPSDTPDVVLVTDQKTHHRGYGSITVAFR
                     RVAGAPGGLMLDGWTVLDAQNNRTTVRLSDQRFNVPIDDSAFRWTDPRGVGPQH"
     gene            2028..2819
                     /locus_tag="VKQ27_20995"
     CDS             2028..2819
                     /locus_tag="VKQ27_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLY91473.1"
                     /translation="MPTTKIASWNINSVRARIDIVQRFLIEAQPDVLCLQETKVEDGT
                     FPHAPFERLGYTHRLLAGQRMHHGVAILSKLPLGEHGRHDWQDNGEARHVGARLPNGI
                     RLENVYIPAGGDIPDPALNPKFAQKLAFVERMTKWSEDLREPTLIVGDFNIAPLESDV
                     WSHKQLLDVVSHTPVEVDALGRLQAAHDWVDLGRHFIPHPQRLYTWWSYRAKDWAASD
                     RGRRLDHMWASPEVAKTATAHRVHEDCRSWLKPSDHIPLVTEFAW"
BASE COUNT          471 a         1030 c          950 g          465 t
ORIGIN      
        1 tgtcgcactg cccaacgacg gagggctgtt cgtggccggc atcgtccgga tccgccacac
       61 gcgcgtcggc gtggtgcgga tcggcgcgtt ccagccacag gcgatgccgg cgctgtgcac
      121 ggccgcgctc gcagcgctcg ccatcccgcc cgacgcgccc tgcgacgacg cctgtcagga
      181 tcgcatcctc atctgggccc atgatcgcat gacccatacg ctcgccgatc ggctggaggc
      241 cctacgcaag gccggcgccc gcgtgctgat ggtcgacatc agcgacaatg gcggcggcac
      301 cgaatgggcc gaagcagcgg cgcgcatggt gagcccggtg acgatccgtt cggaggcgct
      361 ggggttcgtg cgcggcgcac attgggagac acgctggcgg aaccacgccg acggattgcg
      421 cgaggcggcg gcgcaggccg cgccggaaga ccgagcccgg ctgcttgact gggcggctga
      481 ggccgacgct gcccgcgccc tcgccgcgac gccctgcgct gcccgatcga tctgcatcgg
      541 ccgcgccggc ttcgcgaccg ggctggtcag cgaggccccg gccgcctcct ttgccggcaa
      601 accctgggcc gatctggtct tctcgcccgc ccaatatccc tatcgcgatt ccgtctggcg
      661 cggaccactg atcgtgctgg tcgacgacga tacggggtcc gctgccgagg agttcgcggc
      721 ggttctgcag gacaaccgcg ccgcgatcat cctcggccag cggaccggcg gggcaggctg
      781 cggccataca gatggcggaa cacccaccgc gctctccaac agcggcgcgg tgctcgagct
      841 tcccgactgc atccgcttcc gcgccgacgg ctccaacgag gtggacggcg tgatccccga
      901 tctgcccgtg gcgatgcggg ccagcgatgg tccggcgcgc gaagcccaac tcgtcgagcc
      961 atggctggcc caggcggtgt cgcgggcgcg ccgcttgcgt tcgacgccgc gatgacgcca
     1021 tagccgggca gggcggcgga tcggtcgcgc ccatgggttc agcgcctatt caaaccgcgt
     1081 cgcccatccg ggcggggtat cttcggagag atcgatgccg ttgttccgtc ctgcccttgc
     1141 gttcgcgctg gccagcgcct cgttgatcac gccagcaccg gtggcagcca agccggcccc
     1201 ggaaaccccg ctgtcgccgg atctcgcgca ggtgcagacg catctccgct ccgtggcgac
     1261 gatgaccgcc ggcttcgtcc agaccaatcg cgatggcgcg acggtggccg gcacgctcca
     1321 gctcaagcgg cctggcaggg tgcgattcca gtatcaggaa ggcgtgccgc tgctggtggt
     1381 gggcgacggc aaggcgctca tcatggtcga ctataaggtg aagcaggtgt cgcgctggcc
     1441 ggtcggcaat acgcctctgg ggttgctgct caatccggag aaagacgtcg gccgctatgc
     1501 acgcatcgtg ccgagcgaca cacccgacgt cgtgctcgtc accgaccaga agacgcacca
     1561 tcgcggctat ggcagtatca ctgtcgcttt ccgtcgtgtc gcgggtgccc ccggcgggct
     1621 gatgctcgat ggatggaccg tactcgacgc gcagaataat cgcaccacgg tcagactcag
     1681 cgaccagcgc ttcaacgtgc ccatcgacga ttcggccttc cgctggaccg atccgcgcgg
     1741 tgtggggccg cagcattgac gatgaacggt gccgctgcaa cggcgcgccg agtcccggcg
     1801 ccccgtaacc ggggaccgtt catctgcggg acagctaggc agcagtaaat cctccactgc
     1861 ggccacgaag cccatccagg attccccctg ttgcctggac gatacgcttc gtgtcccgcc
     1921 tgcgtgacga acgctaggtc ggcccccgtt ccatgccccc ggaacggggg cctttccttt
     1981 tccggttcgg ctggcgaagc ggccccggac ccattacatc gccgacaatg cccaccacca
     2041 agatcgcctc ctggaacatc aactccgtcc gtgcacggat cgatatcgtc caacgcttcc
     2101 tgatcgaggc gcagcccgac gtgctgtgcc tgcaggaaac caaggtggag gacggcacct
     2161 tcccgcacgc cccattcgag agactgggct acacgcatcg tctgctcgcc ggacagcgca
     2221 tgcatcacgg cgttgccatc ctcagcaagc tgccgctcgg cgagcatggc cgccatgact
     2281 ggcaggacaa tggcgaggcc cgccatgtcg gcgcacggct gcccaacggc atccggctgg
     2341 aaaatgtcta catcccggcc ggcggcgaca tccccgatcc agcgctgaat cccaagttcg
     2401 cgcagaagct cgccttcgtc gaacgcatga cgaaatggtc tgaggatctg cgcgagccga
     2461 cactgatcgt cggcgacttc aacatcgcgc cgctcgaatc cgacgtgtgg agccacaagc
     2521 agctgctcga cgtcgtcagc cacacgccgg tcgaggtcga cgcattgggt cgcctgcagg
     2581 ctgcgcacga ttgggtcgat ctcggccgtc atttcattcc acacccgcaa cgactctaca
     2641 cctggtggag ctaccgcgcg aaggactggg cagcctccga tcgcggccgc cggcttgatc
     2701 atatgtgggc gagccccgag gtcgcgaaga ccgccaccgc acaccgcgtc catgaggatt
     2761 gccggagctg gctcaaaccc tccgatcata tcccgcttgt caccgagttc gcctggtgag
     2821 cgcgcggcgg gagaccgcgc acggtatcga cgcgctgcgg cgcggctggc cgatcgtcat
     2881 cgccgacggc gccgaacggc tggtactgct gccgat
//
LOCUS       DATGEM010000681         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_331663, whole genome shotgun sequence.
ACCESSION   DATGEM010000681 DATGEM010000000
VERSION     DATGEM010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331663"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VKQ27_21000"
     CDS             <1..1618
                     /locus_tag="VKQ27_21000"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLY91474.1"
                     /translation="GSTTSLNNLLLFSQDLSKTAGFLNAINAVRNSSGQIVCSVNADA
                     DPTNDDPACVPISLFGSGNATLTPAALRYINTTAYNRQRASELDITANVSGDLSRWFT
                     TWGGGSLAFNVGGEYRRETASSTWDPLTKAGATFLNALPDFLPPALSSKEGYAEIALP
                     IVKDMRFAKELSINAAARYSDYNTAGGIWSYNVGAIYAPTRDIKFRFGYARSIRTPTQ
                     TDLYATPSQNFGFITDPCDVQNRHTGAATRDANCTAAGIPVDFINDPARTTSLSFLQG
                     GNPGLKPETSDSYTVGTIVTPRWLPGFSLTVDYYNITINKVIQTIDAQQIVDNCYDAA
                     SLNNVYCGLVFRDPTTHLFQDPAVLAGPVNFARQKTAGIDLNLSYAHHFSNGDIFNIQ
                     TIGALVLRRDNYLDVSNPGRVTRQLGNLGDPKWEATGNFDYQHGPIALHYKLHFIGHM
                     YLDDYADYYSLNGEAPQQPEYNLAKKYPVTWYHDLKLTFDVRKGYQFYVGVDNLTDKL
                     PPYGLTGTGNGSAIYDNVGRFFYAGFKVNL"
     gene            complement(1768..3285)
                     /locus_tag="VKQ27_21005"
     CDS             complement(1768..3285)
                     /locus_tag="VKQ27_21005"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HLY91475.1"
                     /translation="MDIALPIDRNRITIAILALGGQGGGVLADWILEVANRNGYVAQG
                     TSVPGVAQRTGSTVYYIEMVRRVAARANHPDPILAMMPVPGDVDIVIASELMEAGRAI
                     LRGFVTDDRTTLIGSTHRIYAISEKSAPGDGTGSSQRILDAAARRAKRFIGFDMDAAA
                     AESGSVISSVMFGALAAADVLPFPREAFEEAIRHGGKAVANNLRGFGFGVERTVSATT
                     KAEAADAPPEATSVVGRTLRDRIHAKLPPAAQPFAIEGASRLMDYQDRAYAELYLNRL
                     DDIRAVDDGRDDGRLTTEVARHLALWMSYEDSIRVADLKVRASRWARVEQEARVDAGQ
                     ILQITEYLHPRLQEVCEILPAWLGRSIMARPRLRKRLEPLFAKGRHVETTSLRWFLVL
                     RLLAALRPIRRSTLRYAEEQARIGAWLRFIRETAISDSALAAEIAACPQLIKGYSDTF
                     ERGLARFETIMEAARTLIGHPDAATRICALREAALADEKGVEFNLLQTRAPLAGS"
     gene            complement(3285..5423)
                     /locus_tag="VKQ27_21010"
     CDS             complement(3285..5423)
                     /locus_tag="VKQ27_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HLY91476.1"
                     /translation="MAERSFQQEVGQLRLGAGDVFEGEGILAVTKALLQAGVAYVGGY
                     QGAPISHLMDVFADADPLLRELGVHFESNASEAAAGAMLAASVNYPLRGAVAWKSVVG
                     TNVASDALSNIASGGVTGGALVIVGEDYGEGSSIMQERTHAFAMKSQIWLLDPRPNLT
                     TIVDMVEKGFELSEASNTPVMIELRVRACHVTGHFLARNNRRPPMTVGEAAANPRRDV
                     DRIVLPPANFLHEQEKIHKRWPAATRFVKEQALNEWFGPEEDEIGIIVQGGLFNTLNR
                     ALELADLSDAYGATRIPVYCLNVTYPLIPEEVARFCTGKKAVLIVEEGQPEFIEHELN
                     TMVRKADLQTRIVGKDILPRAGEYTGQVLRDGIVAFLKAHAPARAPVAITPMPLDPEI
                     AHEVPPRPAGFCTGCPERPIFSAMKLVQRELGEFHVSADIGCHLFSILPPFHIGNTTM
                     GYGLGTAAASAFKPAQGEGARRAIAMMGDGGFWHNGLTSGIGNAVFNQDDTVTIVIDN
                     GYSAATGGQDILSSKAQNPTRNTNHSIEKAARGLGVNWVKTLTRTYDVARMRDTLKQA
                     LTSPEKGPKIIVAQSECMLNKQRRLKPLMARAASEGQRMVRERFGVDPDTCTGDHACI
                     RLSGCPSLSVKANPDPLRSDPVTHIDDSCVGCGNCGEVAHAAVLCPSFYKARIITNPN
                     WGERLRSHLRGAVIGWLQRRHTRAIAARAF"
     gene            complement(5430..>5631)
                     /locus_tag="VKQ27_21015"
     CDS             complement(5430..>5631)
                     /locus_tag="VKQ27_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91477.1"
                     /translation="TDAGQAHLMDPPYPAHTLFHAAGRYGLQCLQNLDQLPPTGAILV
                     AAPLKIVGGSGSPLRVLAFVAE"
BASE COUNT          998 a         1792 c         1831 g         1010 t
ORIGIN      
        1 cggcagcacc acctcgctga acaatctcct gctgttcagc caggacctgt ccaagacagc
       61 gggattcctc aacgcgatca acgccgtgcg caacagcagc ggccagatcg tgtgcagtgt
      121 caacgccgac gccgatccga cgaatgacga tcccgcctgc gttccgatca gcctgttcgg
      181 ttcgggcaac gcgacgctga cgcctgcggc gctgcgctat atcaacacca ccgcctacaa
      241 ccgacagcgc gcctccgaac tcgacatcac cgccaatgtg agcggcgatc tgtcgcgctg
      301 gttcacgacc tggggcggcg gatcgctggc gttcaacgtc ggcggcgaat atcgccgtga
      361 gaccgcgtcc tcgacctggg atccgctgac caaggcgggt gccaccttcc tcaacgccct
      421 gccggacttc ttgcccccgg ccctctcgtc gaaggagggc tatgccgaaa tcgccctgcc
      481 gatcgtcaag gacatgcgct tcgccaagga gctgtcgatc aacgcggcgg cgcgctattc
      541 ggactataac acggccggcg gaatctggtc ctacaacgtc ggggccatct atgcgccgac
      601 gcgcgacatc aagttccggt tcggctatgc gcggtcgatc cgcacgccga cccagaccga
      661 tctgtacgcg acgccgtcac agaatttcgg tttcatcacc gatccgtgcg acgtgcagaa
      721 ccgccacacg ggtgcggcga cgcgcgacgc caactgcact gcggcgggca tccccgtgga
      781 cttcatcaac gaccccgcgc gtacaaccag tctgtccttc ctgcagggcg gcaaccccgg
      841 tctgaagccc gaaacctcgg attcctacac ggttggcacg atcgtgacgc cgcgttggct
      901 gcccggcttc tcgttgacgg tcgattatta caacatcacc atcaacaagg tcatccagac
      961 gatcgacgcg cagcagatcg tcgacaactg ctatgatgcc gcgtcgctga acaacgtgta
     1021 ttgcggcctg gtcttccgcg atccgaccac gcatctgttc caggatccgg ccgtgctggc
     1081 ggggccggtg aacttcgcgc ggcagaagac ggcgggcatt gatctcaacc tcagctatgc
     1141 ccatcatttc tccaacggcg acatcttcaa catccagacc attggtgcgc tggtgctgcg
     1201 tcgggataat tatctcgacg tgtccaaccc gggccgggtc acccgccagc tcggcaatct
     1261 gggcgatccg aagtgggaag cgacgggcaa tttcgattac cagcatgggc cgattgcgct
     1321 gcactataag ctgcacttca tcgggcacat gtatctcgat gattatgccg actattattc
     1381 actcaacggt gaggcgccgc agcagccgga atataatctc gccaagaagt acccggtgac
     1441 atggtatcat gatctgaagc tgacgttcga cgtccgcaag ggttatcaat tctatgtcgg
     1501 tgtcgataac ctgaccgaca agcttccgcc ctacggcctg accggcacgg gcaacggcag
     1561 cgcgatctac gacaatgtcg gtcgcttctt ctacgccggc ttcaaggtga atctctaacc
     1621 gttcggaaca cgcaagacgg ggcggcggtc aaacgaaccg ccgccccttt tttgccatgc
     1681 aggcaagccg catacgcaat gacgaagagc ttcgagctag cccgctcacg cgggcccaga
     1741 gctatggggt tggttcaaac ccccttctca ggaacctgcc aaaggagcac gagtctgaag
     1801 cagattgaac tcgacaccct tctcatccgc cagcgccgct tcacgaaggg cgcagatccg
     1861 ggtggcggcg tcggggtggc cgatcagggt acgcgccgcc tccatgatcg tttcgaaccg
     1921 ggccagaccc cgttcgaacg tatccgaata tcctttgatc agttgcgggc aggccgcgat
     1981 ttcggcggcc agcgcgctat cgcttatcgc ggtttcgcgg atgaaccgaa gccatgcccc
     2041 gatccgcgcc tgctcttcgg catagcgtag cgtcgagcgg cggatggggc gcagggcggc
     2101 gagaagacgc agcaccagga accagcgcag actggtggtc tcgacatgac ggcccttggc
     2161 gaaaagcggt tcgagccgtt tacgtaatcg tggccgcgcc atgatcgagc ggccgagcca
     2221 cgccggcagt atctcgcaca cctcctgcag acgagggtgc agatattccg tgatctgcag
     2281 gatttgcccg gcatcgacac gagcctcctg ttcgacgcgt gcccagcggc tggcgcgcac
     2341 cttgagatcg gccacgcgga tgctgtcttc atagctcatc catagcgcca gatggcgcgc
     2401 gacctcggtc gtgagacgcc catcgtcccg tccgtcgtcg acggcgcgga tatcgtcgag
     2461 gcggttcagg taaagctcgg cataggcacg atcctgatag tccatcaggc gtgaggcgcc
     2521 ctcgatcgca aaaggctgcg cggcgggtgg caatttggcg tggatgcgat cgcggagcgt
     2581 tcgcccgaca acactcgtcg cctcgggcgg cgcatcggct gcctcggcct tggtcgtcgc
     2641 ggacaccgtg cgttcaactc caaagccaaa gcctcgcagg ttgttggcaa cggccttgcc
     2701 gccgtggcgg atggcctcct cgaaggcctc gcgcgggaag ggcagcacgt ccgcggcagc
     2761 gagcgcgccg aacatgaccg agctgatgac gctgccgctc tcggcggctg cggcatccat
     2821 gtcgaagcct atgaagcgct tggcgcgtcg cgccgctgcg tcgaggatgc gctggctgga
     2881 gcccgtgccg tcgcccggcg cggacttctc ggagatggcg tagatgcggt gtgtggatcc
     2941 gatcagcgtg gtacgatcgt cggtgacgaa gccgcgcagt atggcgcggc cggcctccat
     3001 cagctcggac gcgatgacga tgtcgacgtc gccgggaacc ggcatcatgg cgagaatcgg
     3061 atcggggtgg ttggccctgg ccgcgacgcg gcgcaccatt tcgatgtaat agacggtcga
     3121 gccggtgcgc tgcgccacgc caggaacaga tgtgccctgc gcgacatagc cgttgcggtt
     3181 ggcgacctcg aggatccagt cggccagcac gccgccgccc tgcccgccga gcgcgaggat
     3241 ggcgatggtg atccggttgc ggtcgatggg aagcgcgatg tccatcagaa ggctcgagcc
     3301 gcgatcgcac gcgtgtggcg gcgctgtaac cagccaatca cggcgccgcg caggtgcgat
     3361 cgcaggcgtt ctccccaatt cgggttggtg atgatgcgcg ccttgtagaa ggaggggcac
     3421 agcacggcgg cgtgtgccac ttcgccgcaa ttgccgcacc cgacgcagct gtcgtcgata
     3481 tgcgtcaccg gatcggatcg aagcggatcg gggttcgctt tcacgctgag cgagggacag
     3541 cccgagaggc ggatgcaggc atggtctccg gtgcaggtat ccggatcgac gccgaagcgt
     3601 tcgcgcacca tgcgctgtcc ttcgctggcc gccctcgcca tcaggggctt caggcggcgc
     3661 tgcttgttga gcatgcattc ggactgggcg acgatgatct tgggcccctt ctcggggctg
     3721 gtgagggcct gcttgagcgt gtcgcgcatc cgggcgacgt cataagtgcg cgtcagcgtc
     3781 ttcacccagt tgacgccgag gccgcgcgca gccttctcga tcgaatggtt ggtgttgcgg
     3841 gtcggattct gggccttcga cgagaggata tcctgcccac cggtcgcggc cgaatagccg
     3901 ttgtcgatca cgatcgtgac ggtgtcgtcc tggttgaaga cggcgttgcc gatgccggag
     3961 gtaaggccgt tatgccagaa accgccatcc cccatcatcg cgatggcgcg tctggcgccc
     4021 tcgccctgtg ccggtttgaa cgcggaggct gccgcggtgc cgaggccata gcccattgtc
     4081 gtgttgccga tgtggaaagg cggcaggatt gagaagaggt ggcagccgat gtcggcggag
     4141 acgtgaaatt cgcccagctc gcgctgcacc agcttcatcg ccgagaagat cggacgctcc
     4201 gggcagccgg tgcagaagcc ggcggggcgg ggcggaacct cgtgggcgat ctcgggatcg
     4261 agcggcatcg gagtgatcgc gacaggagca cgcgccggcg catgcgcctt gaggaaggcg
     4321 acgataccgt cacgcagcac ctggccggta tattcgccgg cgcgcggcag gatgtccttg
     4381 ccgacgatcc gcgtctgcag gtcggccttg cgcaccatcg tgttgagttc gtgttcgatg
     4441 aattcgggct ggccctcctc gacgatcagc accgccttct tgcccgtgca gaagcgcgcc
     4501 acctcttcgg gaatcaacgg ataggtgacg ttgaggcagt aaaccgggat gcgcgtggcg
     4561 ccataggcgt cggacaggtc ggccagctcg agcgcgcggt tgagcgtgtt gaacaggccg
     4621 ccctggacga tgatcccgat ctcgtcctcc tcgggcccaa accattcgtt gagcgcttgc
     4681 tccttcacga agcgggtcgc ggcgggccag cgcttgtgga tcttttcctg ctcgtggagg
     4741 aagtttgcag gcggcagcac gatccggtcg acatcgcggc gcggattggc cgccgcctcg
     4801 ccgaccgtca ttggcgggcg cctgttgttt ctggcgagaa aatggccggt gacgtgacag
     4861 gcgcgcaccc tgagctcgat catcaccggg gtgttggagg cctcggagag ctcgaagccc
     4921 ttctccacca tgtcgacgat ggtggtgagg ttgggacgcg ggtcgagcag ccagatctgg
     4981 ctcttcatcg cgaaagcgtg ggtgcgctcc tgcatgatcg aggagccttc gccatagtcc
     5041 tcgccgacga tcaccagcgc gccgcccgtg acaccgccgc tcgcgatatt ggagagcgcg
     5101 tccgaggcga cgttggtgcc gaccaccgac ttccatgcca ccgcgccgcg caagggatag
     5161 ttgaccgagg ccgcgagcat ggcgcctgcc gccgcctcgc tcgcgttcga ttcaaaatgg
     5221 acgccaagct cgcgcagcag cggatccgcg tcggcgaaga cgtccatcag atggctgatc
     5281 ggcgcgcctt gatatccacc gacataggcg acgcccgctt gcagcagcgc cttggtgacg
     5341 gccaggatgc cttcgccttc gaagacgtca cccgcgccga ggcggagctg ccccacctct
     5401 tgctggaagg agcgctcggc catgttcgct tactccgcaa caaaggcgag aacgcggagc
     5461 ggactccccg atccgccaac gatcttgagc ggtgcggcaa ccagtatcgc gcccgtcgga
     5521 ggcagctggt cgagattctg gagacactga aggccgtagc gcccggcggc gtggaagagg
     5581 gtgtgggcgg ggtagggcgg atccatcagg tgcgcctggc cggcatcggt g
//
LOCUS       DATGEM010000682         5187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_960911, whole genome shotgun sequence.
ACCESSION   DATGEM010000682 DATGEM010000000
VERSION     DATGEM010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960911"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="rpsC"
                     /locus_tag="VKQ27_21020"
     CDS             complement(<1..378)
                     /gene="rpsC"
                     /locus_tag="VKQ27_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLY91478.1"
                     /translation="MGHKVNPVGMRLGINRTWDSRWFAGDDYSQLLHEDLKLRGHLRK
                     KLHGAGVSRVVIERPAKKPRVTIYAARPGVVIGKKGQDIEVLKRDLGKMANAEVALNI
                     VEIRKPEIDATLVAENIAQQLERR"
     gene            complement(379..780)
                     /gene="rplV"
                     /locus_tag="VKQ27_21025"
     CDS             complement(379..780)
                     /gene="rplV"
                     /locus_tag="VKQ27_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLY91479.1"
                     /translation="MSKPKHPRSLADSEAQAIVSNLRVSPRKLNLVAGMIRNLPAAQA
                     VATLTFSKRRIAQQVKKALESAIANAENNHQLDVDRLVVSRAEVGRSIVMRRFHARGR
                     GRASRVEKWFSHLKIVVAERAQEVEQQSGAA"
     gene            complement(777..1055)
                     /gene="rpsS"
                     /locus_tag="VKQ27_21030"
     CDS             complement(777..1055)
                     /gene="rpsS"
                     /locus_tag="VKQ27_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLY91480.1"
                     /translation="MARSVWKGPFVDGYLLNKAEAARNSSRNEIIRIWSRRSTILPQF
                     VGLTFGVYNGHKFLPVQVNENMVGHKFGEFSPTRTFTGHSGDKRAKRS"
     gene            complement(1057..1887)
                     /gene="rplB"
                     /locus_tag="VKQ27_21035"
     CDS             complement(1057..1887)
                     /gene="rplB"
                     /locus_tag="VKQ27_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLY91481.1"
                     /translation="MALRQFNPVTASLRGTVLINRSELWKGKPVKALTEGKHSTGGRN
                     NHGHITSRFMGGGHKQSYRYVDFKRRKFDVPATVERLEYDPNRTAFIALVKYQDGELA
                     YIIAPQRLRVGDSVVAGQRADIKPGNAMPMAAIPVGTIIHNIEMKPGAGGKIARSAGT
                     YAQLVGKDAGWAQIKLASGELRVVRAECMASIGAVSNPDNMNQKIGKAGRSRWLGRMP
                     HNRGVVMNPVDHPLGGGEGRSSGGRHPVTPWGKPTKGYKTRSNKRTDKMIIRRRNKGK
                     "
     gene            complement(1904..2215)
                     /locus_tag="VKQ27_21040"
     CDS             complement(1904..2215)
                     /locus_tag="VKQ27_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLY91482.1"
                     /translation="MSKAKLSREAMYQIIRSPLITEKATQLSEHGQFVFRVAIKATKP
                     EIKAAIEGLFGVSVIAVNTLVQKGKTKRFKGRPGQRSDVKKAFVKLAPGQSIDFTTGL
                     A"
     gene            complement(2212..2871)
                     /gene="rplD"
                     /locus_tag="VKQ27_21045"
     CDS             complement(2212..2871)
                     /gene="rplD"
                     /locus_tag="VKQ27_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLY91483.1"
                     /translation="MQIEITTLDKTSAGSAELPDEIFATVPRADIMARVVHWQLAKRR
                     AGTHKIKGLSEVSGTTKKPYRQKGTGNARQGSLRAPQFRGGGIVHGPVPRSHEYSLNK
                     KVRRLGLISALSQKQVDGKLVVLDAASGATKTSDLAKKLKALGWSSVLIVDGTVDVGF
                     LRASRNIPGIDVLPTVGANVYDILNHDVLAITAAGVEALKQRLNGGAANDAAPAEEAV
                     A"
     gene            complement(2875..3582)
                     /gene="rplC"
                     /locus_tag="VKQ27_21050"
     CDS             complement(2875..3582)
                     /gene="rplC"
                     /locus_tag="VKQ27_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HLY91484.1"
                     /translation="MANQRTGLLAKKLGMTRIFQDDGTHVPVTVLHLDGLQVVSQRTE
                     EKDGYTAVQLGWGRAKVKNVSQPNRGHFAKTKVEPKAKVVEFRVAPDALLEPGATLSA
                     AHFVVGQKVDVCGSTQGKGFAGGMKRWNFSGLEASHGVSISHRSLGSTGNRQDPGKTF
                     KNKKMAGHLGVERVTTLNLEVAAVDAEKGLLMIKGAVPGSKGGYVLIRDAVKRARPAE
                     APFPAALVEAAAETQAG"
     gene            complement(3647..3955)
                     /gene="rpsJ"
                     /locus_tag="VKQ27_21055"
     CDS             complement(3647..3955)
                     /gene="rpsJ"
                     /locus_tag="VKQ27_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HLY91485.1"
                     /translation="MDNQNIRIRLKAYDHRVLDNSTKEIVNTAKRTGARVRGPIPLPT
                     HIERFTVNRSPHVDKKSREQFEIRTHRRLLDIVEPTPQTVDALMKLDLAAGVDVEIKI
                     "
     gene            complement(4031..>5187)
                     /gene="tuf"
                     /locus_tag="VKQ27_21060"
     CDS             complement(4031..>5187)
                     /gene="tuf"
                     /locus_tag="VKQ27_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLY91486.1"
                     /translation="CNIGTIGHVDHGKTSLTAAITKVLAETGGATFTAYDQIDKAPEE
                     RERGITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMP
                     QTREHILLARQVGVPALVVYMNKVDMVDDPELLELVELEVRELLSSYQFPGDDIPIIK
                     GSALCALEDREQEIGEKSVLELMAAVDAYIPQPVRATDRPFLMPVEDVFSISGRGTVA
                     TGRVERGVIKVGEEVEIVGLRPTQKTVVTGVEMFRKLLDQGEAGDNIGALLRGTKREE
                     IERGQVLAKPGSITPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVQL
                     PEGTEMVMPGDNVTMDVELIAPIAMDAQLRFAIREGGRTVGAGVVASITA"
BASE COUNT          802 a         1863 c         1503 g         1019 t
ORIGIN      
        1 gcgccgctcc aactgctgcg cgatgttctc cgccaccagc gtcgcatcga tctccggctt
       61 gcggatctcc acgatgttca gcgccacctc ggcattcgcc atcttcccga ggtcccgctt
      121 cagtacctcg atgtcctggc ccttcttgcc gatcaccaca ccgggccgcg ccgcatagat
      181 cgtcacgcgc ggcttcttcg ccggacgctc gatcaccaca cgcgacaccc ccgccccgtg
      241 cagcttcttc cgcagatgcc cacgcagctt cagatcctca tgcagcaact gcgagtagtc
      301 gtcgccggca aaccaccggc tgtcccacgt gcggttgatg ccgaggcgca tcccgacagg
      361 attgaccttg tgacccatct atgcggcccc cgactgctgt tcgacttcct gcgcgcgttc
      421 cgcgacgacg atcttcagat gactgaacca cttttccaca cgcgaggcac gcccgcggcc
      481 acgcgcatgg aaccggcgca tcacgatcga acgccccact tcggcgcgcg acaccaccag
      541 ccgatcgaca tcgagctgat ggttgttctc ggcattcgcg atcgcgctct ccagcgcctt
      601 cttcacctgc tgcgcgatgc gccgcttgga gaaggtcagc gtcgccaccg cctgggcggc
      661 cggcagattg cggatcatgc ccgccaccag gttcagcttg cgcggcgaaa cccgcaggtt
      721 cgacacgata gcctgcgctt ccgaatccgc gaggctgcga ggatgcttcg gcttgctcat
      781 gaccgcttcg cccgcttgtc cccggaatgg ccggtgaagg tgcgcgttgg cgagaactcg
      841 ccgaatttgt ggcccaccat gttctcgttc acctgcaccg gcaggaactt gtggccgttg
      901 tagacgccga acgtcagccc cacgaactgc ggcagtatcg tcgaccgccg cgaccagatg
      961 cggatgattt cgttgcggct cgagttgcgc gcggcctctg ccttgttcag cagatagcca
     1021 tcgacaaacg ggcccttcca gacggaacgc gccatcttac ttacccttgt tccggcggcg
     1081 gatgatcatc ttgtccgtcc gcttgttgct gcgcgtcttg tagcccttgg tgggcttgcc
     1141 ccacggcgtc accggatgac gtccgccgga actgcgcccc tcgccaccgc ccagcggatg
     1201 gtccacaggg ttcatcacca cgccgcggtt gtgcggcatg cgacccagcc agcgcgagcg
     1261 cccggccttg ccgatcttct ggttcatgtt gtccgggttc gacaccgccc cgatcgaggc
     1321 catgcactcc gcccgcacca cacgcagctc acccgatgcc agcttgatct gcgcccagcc
     1381 ggcatccttg ccgaccagct gcgcatacgt cccggcagac cttgcgatct tgccgcccgc
     1441 ccccggcttc atctcgatgt tgtggatgat cgtgccaacc gggatcgccg ccatcggcat
     1501 cgcattgcct ggcttgatgt cggcccgctg ccccgccacg acactgtcgc ccacccgcag
     1561 ccgctgcggc gcgatgatgt aggccagctc gccgtcttgg tatttcacca gcgcgatgaa
     1621 cgccgtacgg ttcgggtcgt attccagccg ctccaccgtc gccggcacat cgaacttgcg
     1681 gcgcttgaag tcgacatagc gatacgactg cttgtgcccg ccgcccatga agcggctggt
     1741 gatgtgcccg tggttgttgc gcccgccggt gctgtgcttg ccctcggtca gcgccttgac
     1801 cggcttgccc ttccacaact cggaacggtt gatcagcacc gtgccgcgca ggctcgccgt
     1861 gaccggattg aattgtctca gtgccatgtt ccgcgcggcc cccttatgcg agcccggtgg
     1921 tgaaatcgat cgactgccca ggcgccagct tcacgaacgc cttcttcaca tccgaacgct
     1981 ggccgggacg tcccttgaac cgcttggtct tgcccttctg caccagcgtg ttcaccgcga
     2041 tgacgctgac gccgaacagc ccctcgatcg ccgccttgat ctccggcttc gtcgccttga
     2101 tcgcgacacg gaacacgaac tgcccatgct cggaaagctg cgtcgccttc tcggtgatca
     2161 gcggcgagcg gatgatctgg tacatcgcct cgcgcgagag tttcgccttg ctcatgccac
     2221 agcctcttcc gccggcgccg catcgttcgc tgccccgcca ttcagccgct gcttcagcgc
     2281 ctcaacaccc gccgcggtga tcgccagcac gtcgtggttc agaatgtcat agacattcgc
     2341 ccctacagtc ggcagcacgt cgatgcccgg gatgttgcgg ctggcgcgca gaaacccaac
     2401 gtccaccgtt ccatcgacga taagtacgga agaccagccc agcgccttca gcttcttggc
     2461 caggtcactg gtcttcgtgg caccgctcgc ggcatccagc accaccagct tgccatccac
     2521 ctgcttctgc gacagcgccg agatcagccc gagccgccgc accttcttgt tcaggctgta
     2581 ttcatgcgac cgaggcaccg gcccatgcac aatgccaccg ccccggaact gcggcgcgcg
     2641 caggctgccc tgacgcgcat ttcccgtccc cttctggcga tacggcttct tcgtcgtgcc
     2701 ggagacttcg ctcagcccct tgatcttgtg ggtgcccgcg cggcgcttgg ccagctgcca
     2761 atgcaccacc cgcgccatga tgtcggcccg cggcaccgtc gcaaagatct cgtccggcag
     2821 ctcagcgctg cctgcggacg tcttatcgag cgtggtgatt tcgatctgca tcgttcagcc
     2881 cgcctgcgtt tcggctgccg cttcaaccag cgccgccgga aacggcgcct cggccggacg
     2941 cgcgcgcttc accgcatcgc ggatcagcac atagccgccc ttcgacccag gcaccgcgcc
     3001 cttgatcatc agcagtccct tctcggcatc cacagccgcc acttccaggt tcagcgtggt
     3061 gacacgctcg acaccgaggt gcccggccat tttcttgttc ttgaaggtct tgccgggatc
     3121 ctgccggttg ccggtggaac ccaggctgcg atggctgatc gacacgccgt ggctcgcctc
     3181 aagaccagag aagttccacc gcttcatgcc gccggcgaag cccttgccct gcgtggaacc
     3241 gcagacatcg accttctgcc ccaccacgaa atgcgccgcc gagagcgtgg cgcccggttc
     3301 cagcagcgca tcaggcgcga cccggaactc caccaccttc gccttcggct ccaccttcgt
     3361 cttggcgaag tggccgcgat tcggctgcga cacgttcttc accttggcgc ggccccagcc
     3421 aagctgcacc gcggtgtagc cgtccttctc ctcggtccgc tgcgagacga cctgcaggcc
     3481 atccagatgc agcacggtga ccggcacatg cgtgccgtca tcctggaaga tgcgggtcat
     3541 gccgagcttt ttcgccagca gccctgtgcg ttgatttgcc atggtcatct ttggttccgg
     3601 gcgccagccc ggtcctcaac cgtcatggcc gggcctgacc cggccatcag atcttgatct
     3661 cgacatcgac gccggcggcc aggtcgagct tcatcagcgc gtccaccgtc tgtggtgtcg
     3721 gttccacgat atccagcaac cgccgatgcg tccggatttc gaactgctcg cggcttttct
     3781 tgtccacgtg cggcgagcgg ttcacggtga agcgctcgat atgagtcggc agcgggatcg
     3841 gtccgcggac ccgcgcaccc gtgcgcttgg ccgtgttgac gatctccttc gtgctgttgt
     3901 ccagcacgcg gtgatcgtag gccttaagcc ggatgcggat attctggttg tccatctcaa
     3961 tcttccgagg gttccgcaga aacgcgcggc gccccacctt ccggcggggc gccacctttc
     4021 atccaatcag ctaggcagtg atgcttgcga cgacgccggc accgacggtg cggccacctt
     4081 cgcgaatggc gaagcgaagc tgcgcgtcca tcgcgatcgg cgcgatcagc tccacgtcca
     4141 tcgtcacgtt gtcgcccggc atcaccatct cggtgccttc cggcagctgc acgacacctg
     4201 tcacgtcggt ggtgcggaaa tagaactgcg ggcgatagtt ggtgaagaat ggcgtgtgac
     4261 ggccgccctc ttccttggtc aggatatacg cctcagcctt gaacttcgtg tgcggcgtga
     4321 tcgagccggg cttcgccagc acctggccac gctcgatttc ctcgcgcttc gtgccgcgga
     4381 gcagcgcgcc gatgttgtca cccgcctcgc cctgatcgag cagcttgcgg aacatctcca
     4441 cgccggtcac cacggtcttc tgcgtcggac gcagaccgac aatctcgact tcctcgccga
     4501 ccttgatcac gccgcgctcg acgcgcccgg tcgcgacggt gccacggcca gagatcgaga
     4561 acacatcttc caccggcatc aggaacggcc ggtcggtggc gcgcaccggc tgcgggatat
     4621 aggcatcaac cgcggccatc agctccagga ccgacttctc gccgatctcc tgctcacggt
     4681 cttccagcgc gcacagcgcc gaacccttga tgatgggaat gtcgtcgccc gggaactgat
     4741 aggacgacag cagttcgcgc acctcgagct cgaccagttc gagcagctcg gggtcgtcca
     4801 ccatgtccac cttgttcatg tagacgacga gcgccggcac gccgacctgg cgggcgagca
     4861 ggatgtgctc gcgggtctgc ggcatcgggc catcggcggc ggacaccacc aggatggcgc
     4921 cgtccatctg cgcggcgccg gtgatcatgt tcttcacgta gtcggcgtgg ccagggcagt
     4981 cgacgtgcgc ataatggcgc ttctcggtct catactcgac gtgcgccgtc gagatggtga
     5041 tgccgcgctc ccgctcttcc ggcgccttgt cgatctgatc ataggcggtg aacgtcgcgc
     5101 cgccggtctc ggccagcacc ttggtgatcg ccgccgtgag cgacgtcttg ccgtggtcga
     5161 cgtgaccgat ggtgccgatg ttgcagt
//
LOCUS       DATGEM010000683         4636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_63431, whole genome shotgun sequence.
ACCESSION   DATGEM010000683 DATGEM010000000
VERSION     DATGEM010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63431"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..958
                     /locus_tag="VKQ27_21065"
     CDS             224..958
                     /locus_tag="VKQ27_21065"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLY91487.1"
                     /translation="MLRYGQFCPVAKTAEIFGDRWSPLIIRELCYGPRSFGDFLAALP
                     LISRTMLSQRLKELAAADVVHADAKEKGRGYSYRLTAAGEAFRPMIDMMAEWGLKWGQ
                     GLIGPDDLDSKLLIWGIRQHIEQEDIAAQGFVLRFDFRGIPKGSRTPRHWWMLIRRDE
                     IEVCLKDPGQNLDVLIEADLGAFTKVWTGYSRLEEALARGLVTFRGNDSAIDQMNRML
                     RLTDETKWRDRGFVAPAPERPLSSRS"
     gene            complement(960..1376)
                     /locus_tag="VKQ27_21070"
     CDS             complement(960..1376)
                     /locus_tag="VKQ27_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HLY91488.1"
                     /translation="MLDVRKLTDDVFVAPQIEASDIPALAARGFRTIICNRPDGEALE
                     QPSFSEIEKAATAHGVTIRYQPVQSGSISDADVLVFQEAINGLPKPLLAYCKSGTRCT
                     QLWSLSEAGQRSVQDILGRTQAAGYDMSGLLHRLEP"
     gene            complement(1425..2279)
                     /locus_tag="VKQ27_21075"
     CDS             complement(1425..2279)
                     /locus_tag="VKQ27_21075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLY91489.1"
                     /translation="MSGTKPAFVFVHGGFVDKSIWQLVIPLLEARGHLARALDLPGSA
                     AHAMVPVAYGKRPLDTQAFATEPSPKAHITQEERTQAVVSLVEETARETGGPVVLVGQ
                     SLGGLTVTAVAEAIPDQLRAIVYISAFLQSPGTPGIALMQHATMASAVLPSLFLADPQ
                     VVGACRIDFLSDDAEYRARIKTALCGDIGDAEFPSILAAATCDEPAGVMLAPAHMTAE
                     RFGRVPRHYVRCLEDRAIPLQGQDFMIAAADSALGSQTHTYALSASHFPFFSQPDALS
                     DILLGVAA"
     gene            2668..3942
                     /locus_tag="VKQ27_21080"
     CDS             2668..3942
                     /locus_tag="VKQ27_21080"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLY91490.1"
                     /translation="MSATSTPIFATPRPVDDGAPAGNPWNATPAADACKRLLAGIQDC
                     APEIVARAAEIEAGRRIPPDLIETLKAIGIFRLFAPESRGGLEFDVPTALKVFRALAR
                     IDGSVGWTVMIGNGGHLLTSLLPTETYDHVYRNGPDMIVAGSVVPAGTAEATPGGFRV
                     TGRWPFASGCLHSGWLVGFCVITEDGKPVAGPAGAAGPPRIRGFFLPTRAWQIEHTWH
                     AAGLKGTGSHHIALREALVPAPNFFDFPDGAPCLPGALYQAPHQLLPLLHGSVTVGMA
                     EGALDDLVALANSGRQQLRAAVPMRDSEMFQGELGRVAADLKAAQALLQVQADSHWRY
                     AMAGTRNEEAKVTQHTQTAAWLAATSVRVADACFALGGGVALYESSPLQRRMRDLHAA
                     AQHAAAQQRNYAGAGRLLLDSAAPAGLSLSNT"
     gene            4036..4431
                     /locus_tag="VKQ27_21085"
     CDS             4036..4431
                     /locus_tag="VKQ27_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91491.1"
                     /translation="MRTENNDVRPSRAARLDADGGGAQTPTSAGQTDPATGNAPSPTR
                     APNVGAPRLNRDRTARALERGIVWESVLLLGVAVVIACLAVVMWEVADFGARFVARAV
                     ATELVTVRAVRVHPANLARPAHDAPPSLA"
BASE COUNT          856 a         1455 c         1519 g          806 t
ORIGIN      
        1 cgactgccgg cgtgctgagc ccgaccatgc gccaaaccat cgaggcggcg gcgcccgcct
       61 tctcctgacc ccggccgccg tccggcacgg ggcctcttcc tccccccaaa cccaggccaa
      121 cgaaggaaat ccaccatgcc ggtccatcag aaaatcgact ccaagggcgc gacccactgg
      181 cgcgtggcca gcaactggcg ccacttgtga tcgcatcgaa ctcatgctca ggtacggtca
      241 gttttgcccc gtcgcaaaga cggccgagat cttcggcgat cgatggtcgc cgctgatcat
      301 acgcgaacta tgctatggcc ccaggtcttt tggcgacttc ttggcggctc tgccgctgat
      361 ctccagaacc atgctgtcgc aacgcctgaa ggagttggcg gccgccgacg tcgttcatgc
      421 cgatgcgaag gaaaagggcc gaggctacag ctataggctg acggcggccg gagaggcatt
      481 tcggccaatg atcgacatga tggccgaatg ggggctcaag tggggacagg ggctgattgg
      541 tcccgacgat ctcgattcga agctgctgat atggggaata cgccagcaca tcgagcagga
      601 agatattgcg gcgcagggat ttgttctgcg cttcgatttc aggggcatcc ccaagggcag
      661 ccgcacgcca cgccattggt ggatgctgat ccggcgagac gagattgagg tgtgcctgaa
      721 ggatcccggg cagaatctcg atgtgctgat cgaggccgac ctgggcgcct ttacgaaggt
      781 ctggacggga tattcccgtc tcgaggaggc tctcgcgcga gggctcgtta cattccgcgg
      841 caatgactcc gcaattgatc aaatgaaccg gatgctgaga ttgaccgatg agaccaagtg
      901 gcgggaccgc ggctttgtgg cgcctgcacc cgagcgaccg ctgtcttcgc gatcctgacc
      961 tacggttcga gtcggtgaag caggccgctc atgtcatagc ccgcagcctg cgtgcgcccg
     1021 agaatgtctt gcaccgaccg ctgtccagct tcgctcaacg accaaagttg ggtacagcgc
     1081 gttccggatt tgcaataggc cagcaaaggc ttcggcaatc cgttgatggc ctcttgaaag
     1141 acaagcacgt cagcgtcgct gatcgaaccg gactgaaccg gctgataccg gatcgtaacg
     1201 ccatgggccg tggcggcttt ttcgatctcg ctgaagctgg gctgttccag cgcctcgccg
     1261 tcgggtctgt tgcagatgat cgtacgaaac ccccgcgcgg caagcgccgg aatatcgctg
     1321 gcttcgatct gcggggccac gaacacgtcg tcggttagtt tgcggacgtc cagcattttt
     1381 tgatctccag tataaggcat cgtctatgca tgcgttcggc cggttcaggc ggctacgccc
     1441 aacaagatgt cgctcagggc atcaggctgt gagaagaaag gaaaatggct ggcgctcaat
     1501 gcgtaggtat gggtttgact gcccagggcg ctgtcggcgg cggcgatcat gaagtcctgg
     1561 ccctggagtg ggatcgcccg gtcctccagg catcgaacgt aatggcgcgg cacccggccg
     1621 aagcgttcag ccgtcatgtg cgccggcgcc agcattaccc cggccggttc atcgcaggtt
     1681 gcagcggcaa ggatcgacgg gaattcggcg tcgccaatat cgccacataa ggcggtcttg
     1741 atccgtgcgc gatactcggc atcgtccgac aggaaatcaa tgcggcaggc gcccaccact
     1801 tgtggatctg ccaggaacag cgacggcaac acagcgctgg ccatggtcgc atgctgcatg
     1861 agggcgatcc ccggcgtgcc gggcgattgc agaaaggccg agatatagac aatcgcgcgc
     1921 agctgatcgg gaatggcttc ggccacggcc gtgacggtca ggccgcctag cgactgcccc
     1981 acgagcacga ccggtccgcc cgtctctcgc gccgtttcct cgacaagaga gacgacggcc
     2041 tgggtgcgct cttcctgcgt gatatgggct ttgggagagg gctcggtggc gaaagcttgg
     2101 gtgtcaaggg ggcgcttccc ataggcgacg gggaccatgg cgtgagcagc tgaacccggc
     2161 aaatcgagcg cccgggctaa atggccgcgt gcctcaagaa gtggtattac cagctgccaa
     2221 attgacttgt cgacaaagcc gccatgaaca aaaacaaaag ccggtttcgt gccagacatt
     2281 gcgtgatatt ccttttgaaa tggatttaca gtgagagtgg atacagtgag gatggaccta
     2341 gacgggaata cccatctcca tcaagtctgt ttttcgtact taggtcgtgg gcccagccgc
     2401 tcggaattgt gcactattct tcgaggcgct cgcggcggcc accgcagcag tgtgacaatg
     2461 gggcggggga ggggttgagg acaacctgtt gaggcgccac agctagccca gcgccgaaaa
     2521 cccgcgccgc cagagcggga tgacttaggc gaaccattca cgttcactgc gtgcgcgccc
     2581 gtgccggcga tcgtgcgcca tgggctcacg acgcccggtc ctccgagttc caaactgatg
     2641 atcgtgtttg gaggcaggag gcccgacgtg tcagcgacat caacgcctat tttcgcaacg
     2701 ccccggcccg tcgacgacgg agcgccagcg ggcaatcctt ggaacgccac gcctgccgcg
     2761 gacgcctgta agcgtctgct cgcgggtatc caggactgcg cgcccgagat cgtggcgcgt
     2821 gcggcagaga tcgaagccgg acgccgcatc ccgcccgacc tcatcgaaac cctgaaggcg
     2881 atcggcatct ttcgcctctt cgcccccgag agccgcggcg ggctggagtt tgatgtgccc
     2941 acggcgctga aggtgttcag agcgcttgcc cggatcgacg gttcggtcgg ctggacggtg
     3001 atgatcggca acgggggcca cctgctgacg tccttgctgc cgacggagac ctacgaccac
     3061 gtctaccgca acgggccgga catgatcgtc gccggttcgg ttgtcccggc gggaaccgcc
     3121 gaggccacgc ctggcggctt ccgggtcact ggacggtggc cgttcgccag cggctgcctg
     3181 cattccggtt ggctcgtggg cttctgcgtc atcaccgagg acggcaagcc ggtcgcggga
     3241 cctgccggcg cggcggggcc gccccgcatc agggggttct tcctgccgac gcgcgcctgg
     3301 cagatcgagc acacctggca cgccgccggg ctcaagggca ccgggagcca tcacatcgcc
     3361 ctcagggagg cgctggtgcc ggcgcccaac ttcttcgatt tcccggatgg cgcgccatgc
     3421 ctgccgggag ccctctatca ggcgccgcac cagctcctcc cgctgctgca tggctcggtc
     3481 acggtgggca tggccgaggg cgcactggat gaccttgtgg cgcttgccaa tagtgggcgc
     3541 cagcagctgc gggctgctgt gccgatgcgg gactcggaaa tgttccaagg cgagctcggc
     3601 cgcgtcgccg ccgacctgaa ggccgcgcag gcgctcctgc aggtccaggc cgacagccat
     3661 tggcgctacg ccatggccgg aacgcggaac gaagaggcga aggtgacgca gcacacccag
     3721 accgcggcat ggctcgcggc caccagcgtc cgggtcgccg atgcgtgctt cgcccttggc
     3781 ggcggcgttg cgctctatga gagctcgccg ctgcaacgac ggatgcgcga cctgcacgcc
     3841 gccgcgcaac acgccgccgc acagcaacgg aactacgccg gggcaggcag gctgttgctc
     3901 gacagtgcgg cgcccgcggg actatccctc agcaacacct gatttctgat gtaacgataa
     3961 tcccaaattt cagaaacgag aatgtagcgc acgggtcgca ttattgtcct tcatcgtgag
     4021 aaggagaata accccatgag gaccgagaat aacgacgtaa ggcccagtcg cgccgcgcgg
     4081 ctcgatgcgg atggtggcgg ggcgcagacg ccgacgagcg ccggccaaac cgatccggcg
     4141 accgggaacg ccccctcccc cacacgcgcc ccgaacgtcg gggcgcctcg cctgaaccgc
     4201 gacaggacgg cgcgggcatt ggaacgtggg atcgtctggg aatcggtgtt gctgctgggc
     4261 gttgcagtgg tcatcgcatg tctcgccgtg gtgatgtggg aggtcgcgga cttcggcgcg
     4321 cggttcgtcg ccagagccgt cgcgacagag ctcgtcacgg tccgtgcagt gcgcgtccat
     4381 cctgcaaacc ttgcgcgccc ggctcacgac gctccgccgt cactggccta acccgcttat
     4441 aggcgcccgg cgcggtttgg ctgagacggc gtggacagcg tcccgggaca ccagaaggag
     4501 agacacgatg ccgagcggaa cccatcgcaa cagcctgcag gaacaggtga tctatggccg
     4561 gccggcgctc gaagcgctga ccgagctcgc gggcgtatat ggcgctcgtc ggctgatgat
     4621 caccagcacc gcctcg
//
LOCUS       DATGEM010000684         3326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_53828, whole genome shotgun sequence.
ACCESSION   DATGEM010000684 DATGEM010000000
VERSION     DATGEM010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53828"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VKQ27_21090"
     CDS             complement(<1..908)
                     /locus_tag="VKQ27_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLY91492.1"
                     /translation="MISALLPNYARADLAFEKGEGAWLWTVDGRRFLDFGSGIATASL
                     GHAHPHLVQTIAEQAAKVMHTSNLYRIPQAERLAQRLVDATFADSVFFCNSGAEANEG
                     MIKMMRRAMSDAGKPERFRFICFEGAFHGRTLATLAATGNSHYLEGFGPVVDGFDHVP
                     FNNMNAVRNAIGGNTCGIIVEPVQGEGGVRPADLQFMRDLRAVCDEYDIILGMDEVQS
                     GIGRTGKLFAHEWSGITPDVMSSAKGIGGGFPLGAVLAKERFAKALVPGTHGTTYGGN
                     PLACAAGNAVLDIILAPGFLEGVERKG"
     gene            complement(1160..1345)
                     /locus_tag="VKQ27_21095"
     CDS             complement(1160..1345)
                     /locus_tag="VKQ27_21095"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HLY91493.1"
                     /translation="MPEVYVHAIEGRTKDQKRALIKDITDAVVKNFGVAADAVLVEII
                     ESSRDNKAKGGVLFSER"
     gene            complement(1363..2370)
                     /locus_tag="VKQ27_21100"
     CDS             complement(1363..2370)
                     /locus_tag="VKQ27_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLY91494.1"
                     /translation="MADWQKRQVGRTNLHVTSMGLGTATLGGSRIPMTHDERLNVVRT
                     GWNGGVRYFDTAPFYGVGAAERAVGDALRDVPRGEWVLSTKVGRLLRPQDSGGKTSDG
                     RIAPLPFEVEYDYSYDGIMRSVEDSYQRLGLAKIDILFVHDIGVYQHGEAANAAHMKA
                     LRESGYRALDELRRSGVVSAVGLGVNEKEVLIEVLKFGDWDAFLLAGRYTLLEQGPLD
                     DLLPMCSARGTSIVVGGPLNSGILAGRDTWNYAAAPADIVQRVNAIRAVCDAHGVPLP
                     AAALQFPLAHPAVAAIIPGPRAAGEFEQNAALMRHDIPAGLWADLRDQKLLHPEAPTP
                     S"
     gene            complement(2428..3213)
                     /locus_tag="VKQ27_21105"
     CDS             complement(2428..3213)
                     /locus_tag="VKQ27_21105"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91495.1"
                     /translation="MTTLAIEGLGKTYADGTEALRGMHLFAHSGEIIALLGGSGCGKT
                     TLLRLAAGLDRPSAGTIAIDGREAAGTHPAISAVFQEPRLLPWLSVSHNIGFGGRRVA
                     SGERQRRIEDLLRRIGLEGYGGRWPKDLSGGQQQRVAIARALIGHPGILLLDEPFSAL
                     DAFTRASLHELVLALWQDLRPTILIVTHDVDEAVFLADRVVVMRPRPGRVHETMTIAL
                     PRPRNKLAAEYDITRRNVLRAIDRSLAEDRVAAAAGGAISPWV"
BASE COUNT          589 a         1209 c          969 g          559 t
ORIGIN      
        1 cccttgcgtt ccacgccttc aaggaagcct ggcgccagaa tgatatccag caccgcattg
       61 cccgcggcgc aggccagcgg gttgccgccg tacgtcgtgc catgcgtccc cggcaccagc
      121 gccttggcga accgctcctt cgccagcacg gcgccgagcg ggaacccgcc gccgataccc
      181 ttcgccgacg acatgacatc aggcgtgatg ccagaccact cgtgcgcgaa cagcttaccg
      241 gtgcgaccga tgccggactg cacttcatcc ataccaagaa tgatatcgta ctcgtcacac
      301 accgcgcgca ggtcacgcat gaactgcagg tccgccgggc gtacgccccc ttcgccctgc
      361 actggctcga cgatgatgcc gcacgtgttg ccaccgatgg cgttgcgcac ggcgttcatg
      421 ttgttgaacg gcacgtggtc aaagccatcg accaccgggc cgaagccttc caggtagtgc
      481 gaatttcccg tggcagccag cgtcgccagg gtgcggccgt ggaacgcacc ctcgaagcag
      541 atgaagcgga aacgctccgg cttgccggca tccgacatgg cgcgccgcat catcttgatc
      601 atcccctcgt tcgcctcggc gcccgagttg cagaagaaca cgctgtccgc gaacgtcgca
      661 tcgaccagcc gctgtgccag gcgctccgcc tgcgggatgc gatacaggtt cgaggtgtgc
      721 atcaccttcg ccgcctgctc ggcgatcgtc tgcaccagat gtggatgcgc atgcccgagc
      781 gatgcggtag cgatgcccga accgaaatcg aggaatcgtc gcccgtccac cgtccacaac
      841 caggcgcctt cgcccttctc aaaggcgagg tcggctcggg catagttcgg cagcagcgcg
      901 gaaatcatgg ggaatgaacc cttcttgtga cgtttccaat ggatcgcccg cccgcgacct
      961 ttcggggcgg gtccaaagga agccgggatc attggcggct gtcaggggcg aagtcaaacc
     1021 gcccacccgc aactcccatc gtacctctgg cagtcagttg accaccccgt cattgaaagg
     1081 accccggcga aggccgggga cgaagcaatc tcgaacggga ttgcttcgcc tggggctcgc
     1141 aaggacaatc tctgcggcgc taccgctcgc tgaacagcac gccgcccttc gccttgttgt
     1201 cgcgcgagct ctcgatgatc tccaccagca cggcatcggc ggcgacaccg aaattcttca
     1261 ccaccgcgtc cgtgatgtcc ttgatcagcg cgcgcttctg gtccttggtg cggccctcga
     1321 tcgcgtggac atagacttca ggcatgatcc gacctccctg gttcaggatg gtgttggtgc
     1381 ttccggatgc agcaatttct ggtcgcgcag atcggcccac aacccagccg gaatatcgtg
     1441 ccgcatcagc gcggcgttct gctcgaattc cccggcagca cgcgggcctg gaatgatcgc
     1501 ggccaccgcc ggatgcgcca gcgggaattg caacgccgct gccggcagag gcacgccgtg
     1561 cgcgtcgcac accgcccgga tcgcattcac ccgttgcacg atgtccgcgg gcgccgccgc
     1621 gtagttccag gtgtcgcggc cggcgaggat gccggagttc aacgggccgc ccaccacgat
     1681 cgacgtacct cgcgcgctgc acatcggcag cagatcgtcc agcggcccct gttccagcag
     1741 cgtgtatcgg cccgccagga ggaacgcatc ccagtcgccg aatttcagca cctcgatcag
     1801 cacttccttc tcgttcaccc cgagcccgac ggcgctcacg acaccgctgc ggcgcaattc
     1861 gtcgagcgca cgataaccgc tctcccgcag cgccttcata tgcgccgcat tggctgcctc
     1921 gccgtgctgg taaacgccga tatcgtgcac gaacaggatg tcgatcttcg ccaggccgag
     1981 gcgctggtag ctgtcctcca ctgaacgcat gatgccgtca tacgagtagt cgtattccac
     2041 ctcgaacggc agcggcgcga tccgcccgtc gctcgtcttg ccgccactgt cttgcggtcg
     2101 caacaggcgc cccaccttgg tggacaacac ccattcaccg cgcggcacat cacgcaatgc
     2161 gtcacccacc gcccgctcgg ctgcgccgac cccatagaac ggtgccgtat cgaaatagcg
     2221 cacgccgccg ttccagccag tcctgaccac gttcagccgc tcgtcatgcg tcatggggat
     2281 ccggctgccg ccgagcgtcg ctgtgccgag ccccatcgac gtcacatgca ggttcgtccg
     2341 cccgacctgc cgcttctgcc agtctgccat catcgctcct ccgccaggtt gcgcgacagt
     2401 gggcgaggcc gatcgcggcg agtcaaatca cacccacggc gatatcgcac cgccggccgc
     2461 ggcagcgacg cgatcctccg ccagagaacg gtcgatcgcg cgcaacacgt tgcgccgcgt
     2521 gatgtcgtat tccgccgcca gcttgttgcg cggccgaggc agcgcaatgg tcatcgtctc
     2581 gtgcacgcgt ccaggcctcg gccgcatcac caccacacgg tcggccagga ataccgcctc
     2641 gtccacatcg tgcgtgacta tcaggatcgt cggacgcagg tcctgccaaa gcgccagcac
     2701 aagctcatgc aggcttgccc tggtgaacgc atcaagcgca gagaacggct cgtccagcag
     2761 cagtatcccc ggatggccga tcagcgcgcg ggcgatggca acacgctgct gctgtccgcc
     2821 ggacagatcc ttcggccagc gcccgccata accctccaat ccgatgcgcc gcagcagatc
     2881 ctcgatccga cgctgacgtt cgccgctcgc tactcggcgg ccaccgaagc caatgttgtg
     2941 cgagacgctc agccacggca gcagccgcgg ttcctggaag accgcgctga tcgccggatg
     3001 cgtgcctgcc gcctccctgc cgtcgatggc gatggttccc gcactcggcc ggtccagccc
     3061 cgcggccaga cgcagcagcg tcgtcttgcc acatccggaa ccgccaagca gtgctatgat
     3121 ctcgccgctg tgcgcgaaca gatgcatgcc gcgcaatgcc tcggtcccat cggcgtaggt
     3181 cttgcccagc ccctcgatcg cgagcgtcgt cacagccgtg ccctcacggt atcctgccag
     3241 cgcaacagcg gtgcggtcgc cccaaccagc acggcatccg acagcttgcc cagcaatgcg
     3301 aacacgatca tcgccaccag gatcac
//
LOCUS       DATGEM010000685         6882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1423734, whole genome shotgun sequence.
ACCESSION   DATGEM010000685 DATGEM010000000
VERSION     DATGEM010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423734"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /gene="cofH"
                     /locus_tag="VKQ27_21110"
     CDS             <1..1357
                     /gene="cofH"
                     /locus_tag="VKQ27_21110"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HLY91496.1"
                     /translation="PTYAFEADRWCAPDIATRVRRAIDAEGWARDDDWAPGLTVLPKP
                     RTPFLERAERAIEQIVSKAVTGHRLDEAGIVRLFASRDADYEHVITAADALRQSVSGD
                     VVRYVVNRNINYTNICYFKCRFCAFSKGKTHEELRGAPYDLALDEVVRRSREAWDRGA
                     TEVCLQGGIHPDYTGETYLGICRAIKAALPDMHIHAFSPLEVTQGAATLGLSLREFLS
                     RLKDAGLSTLPGTAAEILDDETRAIICPDKINTDQWLEVVRTAHQLGLRTTSTIMYGH
                     VERPINWARHLLRLRDLQAETGGITEFVPLPFVHMEAPMYLRGQARKGPTLREAVLMH
                     AVSRLALHPLITNIQTSWVKMGPDGAALCLNAGANDLGGTLMNESISRAAGTQHGQEF
                     RPEAMETLIGSIGRIPLQRTTLYSPTPQERRVASFDAPELAPVVQTPPTRHPVPLSLR
                     A"
     gene            complement(1390..2022)
                     /gene="cofC"
                     /locus_tag="VKQ27_21115"
     CDS             complement(1390..2022)
                     /gene="cofC"
                     /locus_tag="VKQ27_21115"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HLY91497.1"
                     /translation="MTIWAAVPVKEFTGAKQRLSPLLTPQQREALAVAMLEDVLSALA
                     EAPLDGIMVNTLDPRATELARRYGARVVTDDARSGHTGAVTAMARILAAEGHDMLTVP
                     GDIPRVTAAEIAAVVEVRKPAPSLTIVPARDEMGSNAMLCSPPLVMPFRFGDNSYFPH
                     LATARGLGIEPAIVKLPGIGLDIDQPEDVEAFLGAAPLMATRAFMLLRGA"
     gene            complement(2146..3081)
                     /gene="cofD"
                     /locus_tag="VKQ27_21120"
     CDS             complement(2146..3081)
                     /gene="cofD"
                     /locus_tag="VKQ27_21120"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HLY91498.1"
                     /translation="MTVVALSGGVGGAKLALGLSRVLPAEQLLVVCNTGDDFEHLGLC
                     ISPDIDTVAYTLAGVANREVGWGRADETWSFMETLGTLGGETWFRLGDRDLAVHVERT
                     RRLRAGETLTAVTADICARLGVAPRVLPMSDDPVRTRVRTDEGWIDFQEYFVRRRCEP
                     VVHELAFHGASTARAQPDFLAALSDPALKAVVICPSNPFISIEPIFAVPGVRAALADC
                     AAPVIAVSPLIGGRAIKGPTAKMMGELGMTPSAGGVARRYGDLIDGYVCDFADMDQVA
                     GIEARVTLAQTLMTTLEDREALARTVLGAARVLRR"
     gene            complement(3078..3839)
                     /gene="cofE"
                     /locus_tag="VKQ27_21125"
     CDS             complement(3078..3839)
                     /gene="cofE"
                     /locus_tag="VKQ27_21125"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HLY91499.1"
                     /translation="MSSALELFALSGIPMVREGDDLAALIVAALGHRELRAGDVVVVA
                     QKIVSKAEGRAVDLATVTPSAAAEKLGAETGKDPRIVEVILGESTRVVRSRPNLIIVQ
                     HRLGFVMANAGVDQSNVAPADGVHRVLLLPRDPDASAEGLRAKLQQHYGVAVGVVISD
                     SFGRAWRRGTCGIAVGAAGLPSLIDLRGQPDLFGRTLEVSIIGFADEIAAAASLLQGQ
                     AAEAQPVVLVRGLDWTAPTAPAAELVRPLEEDLFR"
     gene            complement(3930..4934)
                     /gene="fgd"
                     /locus_tag="VKQ27_21130"
     CDS             complement(3930..4934)
                     /gene="fgd"
                     /locus_tag="VKQ27_21130"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HLY91500.1"
                     /translation="MLKLGYKASAEQFAPSKLLAFSVLAEQSGFDSVFISDHFQPWKH
                     TDGHAPNALVWLGALGASTQRIVMGTSVLTPTFRYHPSIVAQAFGTLGSMFPGRVVLG
                     VGTGESLNEVPSTRMKWPETKERFARLREACQLIRKLWSEDRVTFEGEYYQTENATIY
                     DKPATPVPIYVAAAGPGVAKYAGRSAQGFICTSGKAWKLYTETLLPSVEEGLKLASKP
                     PANYDRMIEMKVSFDTDRERALQDTRHWAALALTPEEKMSVEDPMQMEKLADALPVER
                     AASRWIVSSDPDEHIEKIRPYVEMGFRHLVFHAPGPDQERFLKLYGEQVLPKLRRRFD
                     "
     gene            complement(4962..5660)
                     /locus_tag="VKQ27_21135"
     CDS             complement(4962..5660)
                     /locus_tag="VKQ27_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HLY91501.1"
                     /translation="MADNDPAWEARKLLRAAHAGTLATSLQGQPFASLVTPACAPDLS
                     VLLLLSDMSEHTRHLRVEPRCSVLVTGAPESANPQTAPRVSITGVAEVVADAALKARY
                     LAIHPYASLYADFGDFALWRVRPLGGLYVGGFGRAARLRAGELTPDPDAVAAIAAADA
                     GIISHCNADHPDALAAIAGSAEPWRMVAVDVDGCDLAAGERVIRVHWSEPVADAGGVR
                     RELVGLVDASRGRQ"
     gene            complement(5650..6525)
                     /locus_tag="VKQ27_21140"
     CDS             complement(5650..6525)
                     /locus_tag="VKQ27_21140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY91502.1"
                     /translation="MVGLRPANSTAKVAPDCGALRTGGEQIVAESVEFQPRRFRTAAK
                     HYLAGRSAYAPRLIELVAGLVGLGTADQVLDLGCGPGMLAGAFAPLAREVVAVDPEPE
                     MLRIAEAQFGGAGRIVFRQGSSFDLSPAWGRFRLVTMGRSFHWMDRADTLRRLDGMIE
                     PGGAVALFDSDMLDVPENAWREDYRAVVRRYASADTSHVRHRGPVWVRHEAFLLESAF
                     NALEQVSVIERRTVTVRQLVDRAFSRSSTAPGRLGDAAPRLAEEIEAVMRPLATDGVL
                     AEVVVTSALLGRRGG"
     gene            6551..>6882
                     /locus_tag="VKQ27_21145"
     CDS             6551..>6882
                     /locus_tag="VKQ27_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLY91503.1"
                     /translation="MKQTIALVDDDRNILTSVQMTLEQEGFQVRTYTDGESALQALNA
                     KPVDLAVLDIKMPRMDGMELLQRLRQRSSMPVIFLTSKDEEVDELMGLRLGADDYITK
                     PFSQRLLI"
BASE COUNT         1286 a         2693 c         1878 g         1025 t
ORIGIN      
        1 tcccacctac gcgttcgaag cagatcgctg gtgcgccccc gacatcgcca cgcgcgtgcg
       61 ccgcgcgatc gatgccgaag gctgggcgcg cgacgatgac tgggcgccag gcctcaccgt
      121 ccttcccaaa ccccgcacgc ccttcctgga gcgtgccgaa cgcgccatcg agcagatcgt
      181 gtcgaaagcg gtcaccggcc atcgcctgga cgaagccggg atcgtgcgcc tcttcgcttc
      241 acgcgacgcc gactacgaac acgtcatcac cgcggccgat gcgctgcgcc agtcggtcag
      301 cggcgacgtc gtccgttacg tcgtcaaccg caacatcaac tacaccaaca tttgctactt
      361 taagtgccgc ttctgcgcct tctccaaagg caagacccat gaagagctgc gcggcgcccc
      421 ctacgacctc gctctcgacg aagtggtgcg tcggtcacgc gaagcctggg accgcggcgc
      481 caccgaggtc tgcctgcaag gcggcatcca tcccgactac accggcgaga cctatctcgg
      541 catctgccgc gccatcaaag ctgcgctgcc cgacatgcac atccacgcct tctcaccgct
      601 ggaagtaaca cagggcgcgg caacgctcgg cctgtccctg cgcgaattcc tgtcgcggtt
      661 gaaggacgca ggcctcagca ccctgcccgg caccgcagcc gagatcctcg acgacgagac
      721 ccgcgccatc atctgccccg acaagatcaa caccgaccaa tggctggaag tggtccgcac
      781 cgcgcatcag ctcggcctgc gcaccacgtc caccatcatg tatggacacg tcgagcgccc
      841 gatcaactgg gcgcgccacc tgctgcgcct ccgcgacctt caggccgaaa ccggcggcat
      901 caccgaattc gtaccgttgc ctttcgtcca catggaagcg ccgatgtatc tacgcggcca
      961 ggcgcgcaag gggccgacgc tgcgcgaggc cgtgctgatg cacgccgtgt ctcgcctcgc
     1021 gctgcatccg ctgatcacca acatccagac ctcctgggtg aagatgggtc cggacggcgc
     1081 ggcgctctgc ctgaatgccg gtgccaacga cctcggcggc acgctgatga acgagagtat
     1141 ctcgcgcgcc gccggcacgc agcacggcca ggaattccgg cccgaggcga tggaaaccct
     1201 catcggctcg atcggccgca tccccctgca gcgcaccacg ctctacagcc ccaccccgca
     1261 ggaacgccgc gtcgcctcgt tcgacgcgcc cgaactggct cctgtggtgc agacaccccc
     1321 cactcgccac ccggtccccc tgtcattgcg agcatagcga agcaatcccg tccgggattt
     1381 gcttcgcccc tacgctcccc gcaacaacat aaacgcccgc gtcgccatca gcggcgcggc
     1441 gcccaggaac gcctccacat cctccggctg atcgatatcc agcccgatgc ccggcagctt
     1501 tacaatcgcc ggctcgatcc ccaagccacg cgccgtcgcc aggtgcggaa agtagctgtt
     1561 gtccccaaac cggaacggca tcaccagcgg cggcgaacac agcattgcat tggagcccat
     1621 ctcgtcacgc gccggcacga tagtcagcga tggtgccggc ttccgcacct ccaccactgc
     1681 cgcgatctcc gccgccgtga cgcgcggaat atcgccaggc acggtcagca tatcgtgccc
     1741 ctcggcagcc agaatgcgag ccattgccgt caccgcgccg gtgtgcccgc tccgcgcatc
     1801 atccgtcacc acacgcgcgc cataacgccg cgccagttcg gtcgcgcgcg gatcgagcgt
     1861 gttcaccatg atgccgtcca gcggcgcctc ggctagcgcc gacagaacat cctccagcat
     1921 cgccaccgcc agcgcttccc gctgctgcgg tgtcagcaat ggcgaaagac gctgtttcgc
     1981 gccggtgaat tccttcaccg gcaccgccgc ccatatcgtc atatgtagtt ccgggcgaac
     2041 gcccggtctt cacagcctgc caccgggctc gacccagtgg tcatgctcgc gccccggcgc
     2101 aagtgccctc gccttcgttt ccgccgaatc ggttcgaccc gctcatcatc gccgcagcac
     2161 gcgcgccgcg cccagcaccg tgcgtgccaa agcctcgcga tcttccagcg tcgtcatcaa
     2221 agtctgcgcc agcgtcaccc tcgcctcgat ccccgcgacc tgatccatgt cagcgaaatc
     2281 gcacacgtac ccatcgatca gatcgccgta ccgccgagcc acgccgccag cactcggcgt
     2341 catgccaagc tcgcccatca tcttcgccgt aggccccttg atcgcgcgtc cgccgatcaa
     2401 cggcgacacc gcgatcaccg gcgcggcgca atccgccagc gccgcccgca cacccggcac
     2461 cgcgaagatc ggctcgatgc tgatgaacgg attggacgga cagatcacca cggccttcag
     2521 cgccggatca ctcagcgcgg ccaaaaaatc cggctgcgcg cgcgccgtcg aggcaccatg
     2581 aaacgccagc tcatgcacca ccggttcgca ccgccgccgc acgaagtact cctggaaatc
     2641 gatccagccc tcatcggtac gcacccgtgt ccgcaccgga tcgtcgctca tcggcagcac
     2701 ccgcggcgca acgccaagcc gcgcacaaat atccgcagta accgccgtca gcgtctcgcc
     2761 ggcccgcaat cggcgcgtcc gctccacgtg caccgccaga tcgcgatccc ccagccggaa
     2821 ccacgtctcg ccccccaacg tcccaagcgt ctccatgaac gaccacgtct cgtccgcgcg
     2881 cccccacccg acctcgcggt tcgcaacacc ggccaatgta tatgcaaccg tatcgatatc
     2941 cggcgatatg cacagcccca ggtgctcgaa gtcgtcgccg gtgttgcaca ccaccagcag
     3001 ctgctccgcc ggcagcacgc ggctcaaacc caatgccagc ttggcgccgc caacgccgcc
     3061 agacagcgcg accacagtca tcggaacaga tcctcctcga gtggccgcac cagctccgct
     3121 gccggtgcag tgggggccgt ccagtccagt ccgcgcacta gcaccacggg ttgcgcttcc
     3181 gccgcctgcc cttgcagcag cgacgccgcg gccgcgatct cgtccgcgaa cccgatgatg
     3241 ctgacctcca gcgttcgccc gaacaggtcg ggctgcccgc gcagatcgat caggctcggc
     3301 aacccggccg cgccgacggc gataccgcag gtgccacgcc gccacgcccg gccgaagctg
     3361 tcgctgatga ccacgccgac agcgacccca taatgctgtt gcagcttcgc ccgcagcccc
     3421 tccgcagaag catccggatc gcgcggcagc agcagcacgc gatgcacccc gtccgccggc
     3481 gcgacgttcg actgatcgac gccggcgttg gccatcacga accccagccg gtgctgcacg
     3541 atgatcaggt tgggccgcga acgcaccacc cgcgtggact cgcccaggat cacctcgacg
     3601 atacgcggat ccttgccggt ctccgcgcca agcttctccg cggccgcaga tggcgtgacg
     3661 gtcgcgagat cgacggcgcg tccctcagcc ttcgagacga tcttctgcgc caccaccaca
     3721 acgtcgcccg cgcgcagttc acgatgcccc aacgccgcaa caatcaacgc ggcaaggtcg
     3781 tctccctcgc gcaccatcgg aatgccggac agcgcaaaca gttccagcgc agagctcatg
     3841 ccgacaccac cgacacccct gtcattgcga gcgcagcgaa gcaatctcga ccatctagtg
     3901 cgcgcattgc tcgcaacgac accaagcggt caatcaaacc gccggcgcag cttcggcagc
     3961 acctgttcgc cgtaaagctt caggaaccgc tcctgatccg gccctggcgc atggaacacc
     4021 agatgccgga agcccatctc cacgtaaggc cggatcttct cgatatgctc atccggatcg
     4081 ctcgacacga tccaccggct ggccgcgcgc tccaccggca gcgcatcggc gagcttctcc
     4141 atctgcatcg gatcctcgac cgacatcttc tcttccggcg tcagcgccaa tgcagcccaa
     4201 tgccgcgtgt cctgcaatgc ccgctcacgg tcggtatcga acgaaacttt catctcgatc
     4261 atccgatcgt aattcgccgg cggcttcgat gccagcttca gaccctcctc cacactcggc
     4321 aacagcgtct ccgtgtacag cttccacgcc ttgccagacg tacagatgaa cccctgcgcc
     4381 gaccgcccgg catacttcgc cacccctggc cccgccgccg cgacatagat cggtaccggc
     4441 gtggccggct tgtcgtagat cgtcgcattc tctgtctggt agtactcgcc ctcgaacgtc
     4501 acccgatctt ccgaccacag cttgcggatc aactggcaag cctcgcgcag cctggcaaaa
     4561 cgctccttcg tctccggcca cttcatccgc gtggacggca cttcgttcag cgactcaccg
     4621 gtccccacgc ccagcaccac tcgtccaggg aacatcgatc ccagcgtgcc gaaagcctgc
     4681 gccacgatcg aagggtggta gcggaacgtc ggcgtcaaca cgctggtccc catgacgatg
     4741 cgctgtgtcg atgcgccaag cgcgcccagc cacaccagcg cattgggcgc atggccgtcg
     4801 gtgtgcttcc acggctggaa atggtcgctg atgaacaccg agtcgaaccc gctctgctcc
     4861 gccagcaccg agaacgccag cagcttggat ggagcgaact gttctgccga ggccttgtat
     4921 cccagcttga gcacgatggg tctcctgttg ttgggcggaa gctactgccg gcccctggag
     4981 gcatcaacca gcccgaccag ctcgcgccgg accccgccag catcagcgac cggctcggac
     5041 cagtgaacgc ggatgacccg ctcgccggca gcaaggtcgc agccatcgac atccaccgcg
     5101 accatccgcc acggctcagc cgaaccggca atggcggcaa gcgcatccgg atgatcggcg
     5161 ttgcaatgcg atatgatgcc cgcatcagcc gcggcgatcg ccgccaccgc atccggatca
     5221 ggcgtcagtt cccccgcgcg caaccgggcg gcgcgcccaa acccgccgac ataaagcccg
     5281 ccaagcggcc gcacgcgcca tagcgcgaag tccccgaaat ccgcatacag cgacgcgtag
     5341 ggatggatgg cgagatagcg cgccttcagc gctgcgtcgg caaccacctc agccaccccg
     5401 gtgatcgaaa cccgcggcgc agtctgcggg ttcgcgcttt caggcgcgcc agtcaccagc
     5461 acagagcagc gcggttcaac acgcagatga cgcgtgtgct ccgacatgtc cgacagcagc
     5521 aacagcaccg acaggtccgg cgcacaggcc ggcgtgacca gcgaagcgaa tggctgccct
     5581 tgcagcgatg tcgccagcgt tccggcatga gccgcgcgca gcagcttccg cgcctcccag
     5641 gctggatcgt tatccgccac ggcggcccag cagtgcgctc gtgaccacta cctcggccag
     5701 taccccgtcc gtcgccagag gccgcatcac agcctcgatc tcctcagcca gcctcggcgc
     5761 cgcatctccc agccgacccg gcgccgtgct cgaccgcgaa aaagcccgat cgaccagctg
     5821 ccgcaccgtc accgtccgcc gctcaatcac cgatacctgc tccagcgcat tgaacgccga
     5881 ctccaataga aatgcctcgt gccgcaccca taccggtccc cggtgccgca catggctcgt
     5941 atccgccgag gcataacgcc gcacgaccgc ccgataatcc tctctccagg cattctccgg
     6001 cacatccagc atatcgctgt cgaacagcgc caccgcccca cccggctcga tcatcccatc
     6061 cagccgccgc agcgtgtccg cccggtccat ccagtggaac gaccgcccca tcgtcaccag
     6121 ccggaaccgc ccccacgcag gcgacagatc gaacgaactc ccctgccgga acacgatccg
     6181 cccggccccg ccgaattgcg cctcggcgat ccgcagcatc tccggctcag gatcgaccgc
     6241 caccacctcg cgcgccaggg gcgcgaacgc acccgccagc atgcccggcc cgcagcccag
     6301 atccagcacc tggtccgccg tccccagccc gaccagcccc gcaaccagct cgatcagccg
     6361 cggcgcatag gcagaccgcc ctgccagata gtgcttcgcc gcggtgcgaa accgacgagg
     6421 ctgaaattcc acgctctccg ccacgatctg ttcacctccc gtccgcaaag cgccacaatc
     6481 cggtgccacc tttgccgtgg aattggccgg acggagaccc accatgaccg tacccccgag
     6541 ggaaacctca atgaagcaga ccatcgccct ggtcgacgac gatcggaaca tcctcaccag
     6601 cgttcagatg acgctggagc aggaaggttt ccaggtccgc acctataccg acggcgaaag
     6661 cgccctgcag gccctgaacg ccaagccggt cgatctcgcc gtcctcgaca tcaagatgcc
     6721 ccgcatggac gggatggaat tgctccagcg cctccgtcag cgcagctcca tgccggtgat
     6781 cttcctcacc tccaaggatg aggaagtgga cgagctgatg ggcctgcgcc tcggtgccga
     6841 cgactacatc accaagccct tctcccagcg cctgctgatc ga
//
LOCUS       DATGEM010000686         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_621935, whole genome shotgun sequence.
ACCESSION   DATGEM010000686 DATGEM010000000
VERSION     DATGEM010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621935"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..153
                     /gene="ccoS"
                     /locus_tag="VKQ27_21150"
     CDS             10..153
                     /gene="ccoS"
                     /locus_tag="VKQ27_21150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HLY91504.1"
                     /translation="MNVILYLLGLGVLLGGGGLAVFLWALRSGQYDDMDGAANRILFD
                     DEK"
     gene            complement(96..1562)
                     /gene="glpD"
                     /locus_tag="VKQ27_21155"
     CDS             complement(96..1562)
                     /gene="glpD"
                     /locus_tag="VKQ27_21155"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HLY91505.1"
                     /translation="MFDLAIIGGGINGCGVARDASGRGLSVLLVEQGDLASATSSAST
                     KLIHGGLRYLEHYAFRLVHEALAEREVLLRAAPHLIRPMRFILPHHAGLRPWWLIRAG
                     LFLYDHLGGRKILPGTRSLDLRNDPILRPEYTKGFEYSDCTVDDSRLVILNARDAAAR
                     GADIRPRTRCIGARRIDGVWHMTLEGGTTETARVLVDAAGPWVTRTVGKTTRRHVRLV
                     KGSHIVVPRLHNDERSYILQNADGRVCFVIPYQADFTLIGTTDEDYHGDPADVSCTDE
                     EAHYLCSAVGEYVRHAPSPSNIVWRYAGVRPLLDDGASKAQEATRDYVLELNGDAVLS
                     VFGGKITTYRRLAEAAMAKLAPQFPNLPGSWTANASLPGGDFPWDSVNTLRTDIARRY
                     PFLKDTTIRRLVHAYGTLTTEMLGDARRLEDLGQAFGADLTEREVDWLANNEWARTTE
                     DILWRRSKLGLRISANGLNAYFSSSNKIRFAAPSISSY"
     gene            complement(1644..>2063)
                     /gene="moaC"
                     /locus_tag="VKQ27_21160"
     CDS             complement(1644..>2063)
                     /gene="moaC"
                     /locus_tag="VKQ27_21160"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLY91506.1"
                     /translation="VGAKPITERTATARARVTMLPATADLIRTGGAEKGDVLGVARIA
                     GIMAAKRTSDLIPLCHPLPISAVTLDLEVTEAAVEIVATVRTTGQTGVEMEALTAASV
                     AALTVYDMCKSVDRGMHIDAVRLTHKAGGKSGEFVQD"
BASE COUNT          341 a          571 c          756 g          395 t
ORIGIN      
        1 cggctcggca tgaacgttat cctgtatttg ctgggtcttg gcgtgctgct tggcggcggc
       61 ggactggcgg tgttcctgtg ggcgctgcgc tctggtcaat acgatgatat ggacggcgcg
      121 gcgaaccgga tcttgtttga cgatgagaag taggcgttga gcccattcgc gctgatgcgc
      181 aatccgagct tgctgcgtcg ccagagtata tcttctgtcg tgcgcgccca ttcattgttc
      241 gccagccaat caacctcgcg ttcggtcagg tccgctccga aggcttggcc aaggtcttcg
      301 agcctgcgcg catctccgag catttccgtt gtcagcgtgc cgtaggcatg cacgaggcgg
      361 cggatcgtcg tgtccttgag gaatgggtag cggcgcgcga tatcggttcg cagtgtattg
      421 acgctgtccc aggggaagtc gccgcccggc agtgaagcgt ttgccgtcca ggagccgggc
      481 aggtttggga actgcggcgc cagctttgcc atggcggctt cggcgaggcg gcggtaggtg
      541 gtgatcttgc cgccgaacac cgacagcacc gcgtcgccgt tcagttccag gacgtagtcg
      601 cgcgtcgctt cctgcgcttt gctggcacca tcgtccagca gtggacgaac accggcatag
      661 cgccagacga tattggaagg ggagggggcg tggcgcacgt attcgccgac agcagagcag
      721 aggtagtgtg cctcctcgtc ggtgcaggaa acgtcagccg ggtcgccgtg gtagtcttca
      781 tcggtggtgc caatcagggt gaagtcggcc tgatagggga tgacgaagca gacgcggccg
      841 tcggcattct gcagtatgta gctacgttcg tcgttatgga ggcgaggcac gacgatgtgg
      901 ctgcctttca ccagtcggac gtggcgcctg gtggtcttgc cgacggtgcg ggtgacccag
      961 ggaccggcgg cgtcgaccag gacacgggcg gtctcggtgg tgccgccttc cagggtcatg
     1021 tgccagacgc cgtcgatgcg acgggcgcca atgcaacggg tgcgggggcg aatgtcggca
     1081 ccccgggcgg cggcgtccct ggcgttcagg atgacgaggc gagagtcatc cacggtgcag
     1141 tcggagtatt cgaagccctt cgtatattcg gggcggagga tcgggtcgtt gcggagatct
     1201 agcgagcgcg tgcccgggag gatcttgcga ccgccgaggt ggtcgtagag gaacaggccc
     1261 gcgcggatca gccaccacgg gcgcagaccg gcgtggtgcg gcaggatgaa gcgcatcggt
     1321 cggatcagat gcggagcggc acggagcagg acttctcgtt ccgcgagggc ttcgtggacc
     1381 aggcggaagg cgtagtgctc gaggtagcgc aggccgccgt ggatgagctt ggtcgaggcg
     1441 gaggaggtgg cgctggcgag gtcaccttgt tcgacgagca ggacagagag gccgcggccg
     1501 gaggcgtcgc gggcgacgcc gcagccgttg atgccgccgc cgatgatggc aaggtcgaac
     1561 aagaataatt atggaaccac agatggacac agatatacac agatcatctg tgtccatctg
     1621 tgtttatctg tggttccatt tccttagtct tgtacgaact caccggactt gccgccggct
     1681 ttatgggtca ggcggacggc atcgatatgc atgccacggt cgaccgactt gcacatgtcg
     1741 tagactgtga gcgcggcgac cgaggcagcg gtgagggctt ccatctctac accggtctgg
     1801 ccggtggtgc gcacggtggc cacgatctcc acggctgctt ccgttacttc caggtccaac
     1861 gtcaccgcgc tgatcggcaa cgggtggcag agggggatca ggtcggacgt gcgcttggca
     1921 gccatgatgc cggcaatgcg ggcgacgccc aggacatcgc ccttctcggc gccgccggta
     1981 cggatcagat cggcagtggc ggggagcatg gtgacgcggg cgcgggcggt ggcggtgcgc
     2041 tcggtgatgg gcttggcgcc gac
//
LOCUS       DATGEM010000687         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1136436, whole genome shotgun sequence.
ACCESSION   DATGEM010000687 DATGEM010000000
VERSION     DATGEM010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136436"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VKQ27_21165"
     CDS             complement(<1..488)
                     /locus_tag="VKQ27_21165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLY91507.1"
                     /translation="MQIGFNAPTSGALIGPDSLRRIITEGEALGFDYVTVSDHIMVPR
                     NLESKYPYTNDGEFPAGTAAAWLEQLATTAYIAALTERLRFVMSVMVVPHRPAVLTAK
                     MLATIDYLSKGRLTLGIGVGWCREEFEAIAAAPFDDRGHVTDEWMAACKALWTQENPR
                     FDG"
     gene            complement(491..1444)
                     /locus_tag="VKQ27_21170"
     CDS             complement(491..1444)
                     /locus_tag="VKQ27_21170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY91508.1"
                     /translation="MQIGCSAPTSGPLIAPDSLVRIATEAEMLGFDYVTVSDHVMMPK
                     SVVSRYPYSESGEFPSGAGAARLEQLATAAFIAAATSKLGIVTSVMVVPHRPAVLTAK
                     MLATIDYLSKGRLTLGIGAGWCKEEFEVIGAAPFDDRGHVTDEWMMVCKELWSSDDPR
                     FDGKYVKFSDVVFTPKPAQKSIPIWVGGESGPALRRTAKYADAWYPIGTNPQFPMDTL
                     TRLKGGVAKLKELTEKAGRNPASVGVAYRVSSNPEAQPKGTVNGERKLFTGGGADYAG
                     DIKALQDAGVTAFDFGLFGPSLDATLGNMRRFKDEVMAKVR"
     gene            1603..2553
                     /locus_tag="VKQ27_21175"
     CDS             1603..2553
                     /locus_tag="VKQ27_21175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HLY91509.1"
                     /translation="MQYGIHIGTRGCLTTRDNVMAVAQRAEALGYTHLGVADHLIVPM
                     QTGVRYPYTADGIWPGAPTGECFDCIATLAFLAGCTQRLKLLTSVAVVPHRPAVLAAK
                     LFMTADVLSGGRVIAGVGTGWMREEFEALGTPPYDERGAVTDEYLTAWQALWNEPQPK
                     LDGKYASFDHVVFAPKPAAKPHLPIWVGGESPPALRRTVKFGDAWYPVSNNQTIRLNT
                     PEKLRDGIARLHRTAEKARRDPATIEVAYLWFGPPVWNAQQTPDGRQLFSGTSSDMLA
                     DAAALEKAGAQTVILYLQRPTIEETLEVIQRFGEEVVGKA"
     gene            2550..>3148
                     /gene="cofG"
                     /locus_tag="VKQ27_21180"
     CDS             2550..>3148
                     /gene="cofG"
                     /locus_tag="VKQ27_21180"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HLY91510.1"
                     /translation="MIDDIETASLDDLMARAAAKRDAAHGRLVSYSRKVFIPLTHLCR
                     DVCHYCTFAKRPRAGKAAFMSDDDVLAVARAGAAAGCTEALFTLGDKPELRYRAAREA
                     LAELGHATTISYLREMCALVLQETGLLPHINPGIMTREEIAALREVSASMGIMLESSS
                     DRLCEPGGVHYGSPDKLPAVRLEMMRIAGELAVPFTTGIL"
BASE COUNT          555 a         1123 c          957 g          513 t
ORIGIN      
        1 ccgtcgaacc gtgggttctc ctgggtccac agcgccttgc aggccgccat ccattcgtcg
       61 gtgacatgac ccctgtcatc gaacggcgcg gcggcaatcg cctcgaactc ctcccggcac
      121 cagccgacgc cgatccccag cgtgagccgc cccttcgaca gatagtcgat cgtcgccagc
      181 atcttcgccg tcagcaccgc gggccgatgc ggcaccacca tcaccgacat cacgaagcgc
      241 agccgctcgg tcagcgccgc gatgtaagcg gtcgtggcca gctgctcgag ccacgccgcc
      301 gcggtgccgg ccgggaactc gccgtcgttc gtgtagggat acttcgactc gagattgcgt
      361 ggcaccatga tgtggtcgct gaccgtcacg tagtcgaagc caagagcttc gccttcggtg
      421 atgatgcggc gaaggctgtc cggtccgatc aaggcgcccg aggtcggtgc gttgaaaccg
      481 atctgcatga tcagcgcacc ttcgccatca cctcatcctt gaaccggcgc atgttgccga
      541 gcgtcgcatc cagcgacgga ccgaacagcc cgaagtcgaa cgccgtgacg ccagcatcct
      601 gcagcgcctt gatgtcgccc gcgtaatccg cgccaccgcc ggtgaacagc ttgcgttccc
      661 cattcaccgt ccccttcggc tgcgcctcgg gattcgacga cacacgatac gccacgccca
      721 ccgatgccgg attgcgcccg gccttctcgg tcagctcctt caacttcgcc acgcccccct
      781 tcagccgggt cagcgtatcc atcgggaact gcgggttggt gccgatcgga taccacgcgt
      841 cagcgtattt cgcggtgcgc cgcaatgccg gaccgctctc gccgccaacc cagatgggga
      901 tcgacttctg cgccggcttc ggcgtgaaca ccacgtcgct gaacttcaca tacttgccgt
      961 cgaaccgcgg atcgtcgctc gaccacagct ccttgcagac catcatccat tcatcggtga
     1021 catgcccgcg atcatcgaac ggcgcagccc cgatcacctc gaactcttct ttgcaccagc
     1081 ccgcgccaat gcccagcgtc agccggccct tcgacagata gtcgatcgtc gccagcatct
     1141 tcgccgtcag caccgccgga cgatgcggca ccaccatcac cgaggtgacg atacccagct
     1201 tcgacgtcgc cgccgcgatg aatgccgcgg tggcaagctg ctccagccgc gccgcgccgg
     1261 cacccgaagg aaactcacct gactcgctgt agggataacg cgacaccacg ctcttcggca
     1321 tcatcacgtg gtcgctgacc gtgacgtagt cgaagccgag catctcggct tcggtggcga
     1381 tgcgcaccag gctgtccggc gcgatcagag gcccgctggt tggcgcgctg cacccgatct
     1441 gcatttgttc cctccgcttg cgttgcgtct gtcctgacgt tagcacacga tccgccgccg
     1501 gaagcgagag gagggcggat ggctggatca ggtccggcca cgaccaccgg gacaagcccg
     1561 gtggcatgcg gagaagtcgg gcttaagccc ggaggaagta acatgcagta cggtatccac
     1621 atcggcacgc gcggctgcct gaccacgcgt gacaacgtca tggccgtggc gcagcgcgcc
     1681 gaggcgcttg gctacacgca tctcggcgtg gccgaccacc tgatcgtgcc gatgcagacc
     1741 ggcgtgcggt atccttacac tgcggacggc atctggcccg gcgcgcccac cggcgaatgc
     1801 ttcgactgca tagcaacgct ggccttcctc gcgggctgca cgcaaaggct taagctgctg
     1861 acctcggtcg ccgtcgtgcc acatcgcccg gcggtcctcg cggcgaaact gttcatgacc
     1921 gccgatgtgc tgtcaggcgg ccgcgtcatc gccggcgtcg gcaccggctg gatgcgcgag
     1981 gaattcgagg cgctcggcac gccgccctac gacgagcgcg gcgccgtcac cgacgagtac
     2041 ctcaccgcat ggcaggcact ttggaacgag ccccagccaa agctcgacgg caaatatgca
     2101 agcttcgacc acgtcgtgtt cgcccccaag cccgcagcga aaccgcatct gcccatctgg
     2161 gtcggtggcg aaagccctcc cgccctgcgg cgcacggtga agttcggcga tgcctggtat
     2221 ccggtatcga acaaccagac gatccgtttg aacacgccgg aaaagctgcg cgacggcatc
     2281 gccaggctgc accgcacggc cgaaaaggcc aggcgcgatc cagccaccat cgaggtcgcc
     2341 tacctctggt tcggcccgcc tgtgtggaac gcacagcaga cgcccgatgg gcgccagttg
     2401 ttcagcggca cgtcctcgga catgctggcc gacgccgcgg ccctggaaaa ggctggcgcg
     2461 cagaccgtga tcctgtatct acagcggccg acaatcgagg agacgctgga ggtgatccag
     2521 cgcttcgggg aagaagtggt aggcaaggca tgatcgacga catcgagacc gcatcgctcg
     2581 acgacctgat ggcgcgcgcg gcggcgaaac gcgacgcggc gcacggacgt ctggtgtcct
     2641 attcgcgcaa ggtgttcatc ccgctgactc acctctgtcg tgacgtttgc cactactgca
     2701 ccttcgccaa acgcccccgc gccggcaagg cggctttcat gtccgacgac gacgtgctgg
     2761 ctgtggcccg tgccggtgcc gcggccggct gcaccgaggc gctgttcacc ctcggcgaca
     2821 agccggagct gcgctaccgc gccgcgcgcg aagccctcgc cgaactgggc cacgcaacca
     2881 cgatctccta tctgcgcgag atgtgcgcac tggtgctgca ggaaaccggc ctgctgccgc
     2941 acatcaaccc gggcatcatg acgcgcgagg aaatcgccgc gctgcgcgaa gtctccgcca
     3001 gcatgggcat catgctggaa tccagcagcg accggctgtg cgaacccgga ggcgtgcact
     3061 acggctcgcc cgacaagctg cccgcggtcc ggctcgagat gatgcgcatc gccggcgaac
     3121 tcgcggtgcc gttcaccacc ggcatcct
//
LOCUS       DATGEM010000688         7806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_672309, whole genome shotgun sequence.
ACCESSION   DATGEM010000688 DATGEM010000000
VERSION     DATGEM010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672309"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VKQ27_21185"
     CDS             complement(<1..669)
                     /locus_tag="VKQ27_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91511.1"
                     /translation="MPPYAEFVCEAYRFDLETGALSLHYKFDDALRFEERIVFPRVDR
                     TLSAVEDEALERIFRMLLLACGVSYYKAYAPDRIRCTAFPVDPATAAFFTEFYVKGLG
                     EYAWRNAVNLAGRVHIVTDAVESASAVQVELPRRTCVPVGGGKDSIVTVECLKQAGEP
                     LVLFSLGNAAPIDATIRQSGLPFIRVTRSLDRRLLELNAAGALNGHVPITGILSMIVL
                     ACAII"
     gene            complement(653..1549)
                     /locus_tag="VKQ27_21190"
     CDS             complement(653..1549)
                     /locus_tag="VKQ27_21190"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HLY91512.1"
                     /translation="MREIIDRIEALIHQEVGRNIEGLFAATKGGLWQGASAMASASAL
                     SVGIITGFYVPLGTPPAAETDGPVGAAMLAAALRQVGVPCRLATDEPCRGTCAAALRD
                     SDVPLDVVPLGGSVDEVVASWRMLGVTHVVSIERCGRSADGAPRNMRGEDISAFTTPL
                     DDLFLAGPWTRIAIGDGGNEIGMGSVPASLIGDFVAHGEKIACATPADHMIVAGVSNW
                     GAYALIAALAMLRNDWRAGLLNCLDPARDEAILRAMVDQGPAVDGVTRRRTLTVDSLP
                     MDIHHAKLRAIRAMATDDASVR"
     gene            complement(1670..2470)
                     /locus_tag="VKQ27_21195"
     CDS             complement(1670..2470)
                     /locus_tag="VKQ27_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HLY91513.1"
                     /translation="MDAMPERGFALGHAERLRIRAGAFTGATSGLAPGNVQANLAILP
                     KALANDFLRFAQANPKPCPVLAVSEPGDPSFPTIGRDIDIRTDVPRYRVWRNGELVEE
                     PTDVSHVWRDDLVSFAIGCSFSFEEALVEDGIEVRHIALGCNVPMYRTSVPCVPAGVF
                     HGPLVVSMRPLRPADAIRAVQITSRFPSVHGAPVHIGLPEHIGIKDINKPDYGDAVPV
                     GADELPVFWACGVTPQAAVAAVKPEFCITHAPGSMLITDLRNSKLAAF"
     gene            2602..3528
                     /locus_tag="VKQ27_21200"
     CDS             2602..3528
                     /locus_tag="VKQ27_21200"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLY91514.1"
                     /translation="MANLIGVLLGLTIGAEALFIWYSREPAPIVAPAIVASAQPTPRI
                     SVQPAAPAPMPHESSPVELPRFEAPLPSQSQAGALPRVVYPEAPSPDTHRPGSAMAGT
                     GFFVASDGSLLTAAHVVTDCKATRIASRFVLPTSVRLIALDKKRDIALLRADHLAPPA
                     TLPIGHPAAPGGRLFVLGYPASGGVLVPTETWGTLENAHLQPAPADFVDPRRLIWAAA
                     PEVAHGFSGGPMLDPRNGAVIGIVRGMVDSTRLHAERAAIPAHGMVIGPGSMALTALL
                     GHEGADDDAIAMSGDDALDAARRATVHVLCLF"
     gene            complement(3590..4027)
                     /locus_tag="VKQ27_21205"
     CDS             complement(3590..4027)
                     /locus_tag="VKQ27_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91515.1"
                     /translation="MHIRKTVAAALLIGSTIAMPAFAYTPGCPASQKTEGEGGAGGNW
                     QSTPGATAQKTEGEGGAGSNWQSAPGATAQKTEGEGGAGGNWQSAPGATARKTEAEGG
                     AGGNYQARPSASAQKTEAEGSAGGTWQPGVGAVAQKTAAAPCP"
     gene            4208..5602
                     /locus_tag="VKQ27_21210"
     CDS             4208..5602
                     /locus_tag="VKQ27_21210"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLY91516.1"
                     /translation="MRTITRIVLAAIALISVGAGAARAGSPTPQELVAHELAAVVSIA
                     VLRGPVPAAPPTMASASAESKTAAQQPSASDMPHDTHILGSGYIIDPGGIIVTNRHVI
                     ERAKDILVILHDNTLLRATVLAQANEVDIAVLKVTPDSPLPAVRFGDSDAVQVADPVL
                     AIGNPLGLGGSVTQGIVSALNRDIRDTPFDDYIQTDAAINHGNSGGPLFNVHGEVIGM
                     NTAIIAPNGTSGSIGLGFAIPSNDVKYVVDSLRSPGGMRLGTLDFRIQQVTPEIADAL
                     NMPKAEGVIVGGTKEDGAASKCGIVAGDIILKYGTMPVTDVRALARAVARSKPGTAVN
                     LLVWRNDEPTVITATVGAMEETAPAVETPVAVVAAAEPGWHLAPIDDGARRAFGVSGG
                     AGVAVARVDAGGPASDAGVAAGDVIVQIQQEKVANAADVARAITSARQQQRHFVIVLV
                     QNAQGLRWLSVSLE"
     gene            complement(5610..7007)
                     /locus_tag="VKQ27_21215"
     CDS             complement(5610..7007)
                     /locus_tag="VKQ27_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLY91517.1"
                     /translation="MAYDVLIVDDEPDIRMLIDGVLRDEGYETRGAADSEAAIAAFRM
                     RRPSLAILDVWLQGSRMDGLGILEAFHSEEPHVPVVMISGHGNIEMAVTAIQQGAYDF
                     IEKPFKSDRLLLVVRRALEAAALARENAELRLRAGPETSLTGDSSAIGVLRTGVERVA
                     PTGSRALIHGPAGSGKEVVARMIHARSRRAEGPFIVLNCATLAPGRFEEELFGLEAGA
                     DPIAQPRRAGVLERAHGGTLLLDEVSDMPLETQGKIVRALQDSTFERLGGSARIKVDV
                     RVLSTTNKDLQTEIASGRFREDLYYRLAVVPLKVPALKERREDVPALSQHFIVRSAEN
                     AGVQSRELSADTLAALQAYDWPGNVRQLRNLMDWLLIMAPGGGADPIRADMLPPEIGS
                     NAPALLNIDPTADIMGLPLREARDLFETQYLQAQLLRFGGNISRTAQFVGMERSALHR
                     KLKQLGVNSDEKVGS"
     gene            complement(6997..>7806)
                     /locus_tag="VKQ27_21220"
     CDS             complement(6997..>7806)
                     /locus_tag="VKQ27_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLY91518.1"
                     /translation="IGPATRRRTLLVRIGADLSGGRTDGFVVTFDDITELQSAQRKAA
                     WADVARRIAHEIKNPLTPIQLAAERLKRRFAKEITTDPDTFKQCADTIIRHVGDIGRM
                     VDEFSAFARMPHPVIKSEDVGRVAREMLVLQKTARPQIDWCTEIPEKGPVAPCDRRLI
                     GQALTNLLQNAADAVAMRESAGHIGLCVQCDQSDVRIIVTDDGVGLPQEDRARLTEPY
                     VTHKPKGTGLGLAIVKKIMEDHGGRLTLEDRTDGPGAVATLVLPIKVPDGV"
BASE COUNT         1398 a         2474 c         2532 g         1402 t
ORIGIN      
        1 gatgatcgcg caggcgagca cgatcatgct gagtatgccg gtgatcggca catggccatt
       61 cagcgcacct gccgcattca gctccagtaa ccggcgatcc aggctgcggg tcacgcggat
      121 gaacggcaag ccggactggc ggatcgtggc gtcgatcgga gccgcgttgc caagtgagaa
      181 cagaaccagc ggctcgcctg cctgcttcag gcattcaacc gtgacgatgg agtccttgcc
      241 gccgccaacc gggacacagg tgcgacgcgg cagctcgacc tgaaccgcgc tggcggactc
      301 gacggcatcc gtgacgatat gcacccgtcc cgccaggttg acagcgttgc gccaggcgta
      361 ttcgccaagg cctttcacat agaactcggt gaagaatgcc gccgtcgcgg gatcgactgg
      421 aaatgcagtg cagcggatgc gatcaggcgc ataggccttg tagtagctga cgccacaggc
      481 aagcagcagc atacggaaga ttcgctcgag tgcttcgtct tccaccgccg acagtgtgcg
      541 atcgactctc ggaaacacga tgcgctcttc gaagcgaagc gcgtcgtcga atttgtagtg
      601 cagtgacagc gcccctgttt ccaggtcgaa gcggtacgcc tcgcagacga attcagcgta
      661 cggaggcatc gtccgtggcc atcgcccgga ttgcacgcag cttggcgtgg tgtatgtcca
      721 tcggcaggct gtcgactgtg agcgtacgtc gcctggttac gccgtcgaca gcggggccct
      781 gatccaccat tgcgcgcagg atcgcttcgt ctcgcgctgg atccagacaa ttgagcagcc
      841 cggcgcgcca gtcgttgcgc agcatcgcga gcgcggcgat cagcgcgtat gcgccccagt
      901 tcgatacgcc ggcgacgatc atatgatcgg ctggtgtcgc acaggcgatc ttctcgccgt
      961 gcgcgacgaa atctccgatg agcgaggcag ggacggaacc catgccaatc tcgttgccgc
     1021 catcgccaat ggcgatgcgc gtccaggggc cggcgaggaa caggtcgtcc agtggcgtgg
     1081 tgaaggcact gatgtcttcg ccccgcatgt tccgcggtgc cccatcggca ctgcggccgc
     1141 accgctcgat cgacaccaca tgtgttacgc cgagcatccg ccacgaggcg acaacttcat
     1201 cgaccgagcc gcccagcggc acgacatcca gcggaacgtc gctgtcacgc agagccgcgg
     1261 cgcaggtccc gcggcaaggt tcgtctgtgg cgaggcggca aggaacgcca acctgccgca
     1321 acgcagccgc gagcatggcc gcaccaaccg gcccgtcagt ttcggccgcc ggaggcgttc
     1381 ccagaggcac gtaaaagccg gtgattatgc caaccgagag tgcggaagcg gacgccatcg
     1441 cggaagcacc ctgccacagg ccgcctttcg ttgccgcgaa caatccctcg atgttacggc
     1501 cgacttcctg atggatcaga gcctcaattc ggtcgattat ctcacgcatg gcgggttcgg
     1561 atcgaactac agatggacac agatagacac agatgaagtg ccgccgtccg gcagcttact
     1621 gtcgacagct caatatctgt atgcatctgt gtccatctgt tgttccaatt caaaatgccg
     1681 ccagcttgga attccgcagg tcggtgatca gcatagagcc aggcgcgtgc gtaatgcaga
     1741 actccggctt caccgccgcg actgccgcct gcggcgtgac accgcaggcc cagaacactg
     1801 gcagctcgtc cgcaccgact ggaacagcgt cgccgtaatc cggcttgttg atgtccttga
     1861 tgccgatgtg ttcaggcaga ccgatatgca ccggcgcgcc gtgcaccgaa gggaagcgcg
     1921 acgtgatctg caccgcgcga atcgcatcgg ccgggcgaag cgggcgcatc gacacgacaa
     1981 gcggcccatg gaagacgccg gccggcacgc atgggacgga ggtgcgatac atcggcacgt
     2041 tgcagccgag ggcgatgtgg cggacctcga tcccgtcctc gaccagcgcc tcttcgaagg
     2101 agaacgaaca gccgatcgcg aaactcacca ggtcgtcacg ccagacgtgg gaaacgtcgg
     2161 tcggctcctc gaccagttcg ccattgcgcc agacacgata gcgcggcacg tcggtccgaa
     2221 tgtcgatatc gcggccgatt gtcgggaacg acggatcgcc cggctcggag accgccagca
     2281 cgggacatgg tttcgggttc gcctgggcga agcgcaggaa gtcgttggcc agtgccttgg
     2341 gcaggatggc caggttcgcc tgcacattgc ctggcgccag gcccgaggtc gcgccggtga
     2401 atgcgccagc gcggatgcgc agccgttcgg cgtgaccgag cgcaaatccc cgttccggca
     2461 tggcatccat caattcctcc atccgactca tctgcacgga cattagcaga cactcgaccc
     2521 gcacggcagc ctcggaggaa tcattcggtt tgcaacacca tggcaactgt tgtttgcgat
     2581 tctgtggcca tgcggccctg gatggcaaac ctgatcggcg tgctgctcgg cctcaccatc
     2641 ggcgccgagg cgctgttcat ctggtattcg cgagagcccg cgccaatcgt ggcgcctgcc
     2701 atcgtcgcgt ccgcgcagcc aacgccacgg atcagcgtgc agccggctgc gcccgcccca
     2761 atgccgcacg agtcgtcgcc ggtcgaattg ccgcggttcg aggcaccgct gccgtcgcag
     2821 tcgcaggccg gggcgctccc tcgcgtcgtc taccccgaag cgccatcgcc tgacacccat
     2881 aggccggggt cggcgatggc gggcaccgga ttcttcgtcg cttcggatgg cagcctgctg
     2941 acagcggcac acgtggtgac cgactgcaag gcgacccgga tcgcgtcgag gtttgtcctg
     3001 ccgaccagcg tccgcctgat cgccctggat aagaagcgag acatagcgct gctgcgggcc
     3061 gatcacctcg ctccgccggc gactttgccg atcggacatc cggccgcacc gggtggtcgg
     3121 ttgttcgtgc tgggctatcc tgccagcggg ggcgtgctcg tccccacgga aacctggggc
     3181 accttggaga atgcgcatct gcaacccgca cccgccgact tcgtcgaccc gcgccggctg
     3241 atctgggcgg cagcgcccga ggtcgcgcat ggcttcagcg gcggcccgat gctggatcca
     3301 cgcaatggcg ccgtcatcgg tatcgtgcgc ggcatggtgg acagcacacg cctgcatgcg
     3361 gaacgtgcgg cgatcccggc ccatgggatg gtgataggcc caggctccat ggcgctgacc
     3421 gcgcttctcg gccatgaagg cgcggatgac gacgcgatcg ccatgtcggg cgatgatgcg
     3481 ctggatgcag cacggcgcgc gaccgtgcac gtgctttgcc tgttctgagc ggcatcgacc
     3541 gctccggctt gcgttggtct gcaaacagga gcggacgcgt gtggccgcat tacgggcagg
     3601 gtgcggcggc agtcttctga gcgacggcac caactccggg ttgccaggtt ccgccggcgc
     3661 taccttccgc ctcggtcttt tgcgcggatg cgctcggtcg ggcttggtaa ttgccaccgg
     3721 cgccaccctc agcctcggtt ttccgtgccg tagcgcctgg ggctgattgc cagttgccac
     3781 cagcgccgcc ctcgccctcg gtcttctgtg ccgtagcgcc tggggctgat tgccagttgc
     3841 taccggcacc gccctcaccc tcggtcttct gtgccgtagc gcctggggtt gattgccagt
     3901 tgccaccggc accgccctcg ccttcggtct tctgtgaagc cgggcagcct ggcgtatagg
     3961 cgaaggccgg catggcgatc gtgctgccaa tcagcagtgc agcagcgacg gtcttccgga
     4021 tgtgcatggt caagtcctcc gttgtgtttc ggcgtaatgc cgacgtgagg accgaacggc
     4081 tgacggcttt attcgccagg cgatcattga atttctgtca tcaagtcatg cgacctcgag
     4141 gcgaataaat gcagatgaag tctggtctct gacgcattgg cactatgcaa ggtcaggagg
     4201 cgactccatg cgtaccatca ctcgtatcgt tctcgctgct attgcgctca tcagtgtagg
     4261 tgcaggcgcc gcgcgcgccg gcagtccgac accgcaggaa ctggtcgcac acgagttggc
     4321 ggctgtcgtc agcattgccg tgttgcgcgg gcccgttcca gccgcgccgc ccacgatggc
     4381 gagcgccagc gccgaatcca agacggcggc gcagcagccg tccgcatcgg acatgcccca
     4441 cgacacgcac atccttgggt cgggatacat catcgatcca ggcgggatca tcgtgaccaa
     4501 ccggcacgtc atcgagcgtg ccaaggacat cctggtgatc ctgcatgaca atacgctgct
     4561 gcgtgcgacg gtactggcgc aggcaaacga ggtggatatc gcggtgctca aggtcacacc
     4621 ggattcacca cttccggcgg tgagatttgg cgacagcgat gcggtgcagg tggccgatcc
     4681 ggtattggcg atcggcaatc cactgggcct tggcgggtcg gtgacgcaag gcatcgtcag
     4741 cgcgctcaat cgcgatatcc gagatacacc gttcgacgac tacatccaga cagatgcggc
     4801 gatcaatcat ggcaattccg gtggtcctct gttcaacgtg cacggcgagg tcatcggaat
     4861 gaacacggcg atcatcgcgc cgaacggaac cagtggttcg atcgggctcg ggttcgcgat
     4921 accgtcgaac gatgtgaagt atgtggtcga ttcgctgcgc agtcccggcg gaatgcgtct
     4981 cggcacgctc gacttccgca tccagcaggt gacgccggag attgccgatg ccctgaacat
     5041 gccaaaggcc gaaggcgtga ttgttggcgg cacgaaggaa gacggagcgg cgagcaagtg
     5101 cggtatcgtc gcgggcgata tcatcctgaa gtacgggacg atgccggtga cggatgtgcg
     5161 ggcgctggcg cgcgccgtgg cacggtcaaa gcccggcacc gcggtcaacc tgctggtttg
     5221 gcgcaatgac gaaccgacgg tgatcacggc gaccgtcggg gcgatggagg agaccgcgcc
     5281 ggctgtcgaa acccctgtag cggtggtggc ggcggccgaa ccaggctggc atctggcacc
     5341 gatcgacgac ggcgcgcggc gcgcgttcgg tgtgtctggc ggagcgggcg ttgcggtggc
     5401 acgcgttgat gcaggtggtc cggcatctga cgccggcgtg gccgcgggtg atgtgatagt
     5461 ccagatccag caggagaagg ttgcgaatgc agcggatgtc gcgagggcga tcacctctgc
     5521 acgccagcag cagaggcact tcgtcatcgt gctggtgcag aatgcgcagg gcctgcggtg
     5581 gctgtcggtc agtctggagt agctgggcct cagctcccca ctttttcgtc ggagtttacg
     5641 ccgagttgct tcagcttgcg gtgcagcgca ctgcgctcca tgccgacaaa ctgggcggtc
     5701 cggctgatgt tgccgccgaa gcggaggagt tgtgcctgca gatattgtgt ctcgaacaga
     5761 tcgcgcgcct cgcgcagtgg caggcccatg atgtccgcgg tcggatcgat gttcagcagc
     5821 gcgggcgcgt tggagccgat ttcaggtggc agcatgtcgg cgcggatcgg gtcggcgccg
     5881 ccgcccggcg ccatgatcag cagccagtcc atcaggttgc gcaactggcg cacgttgccc
     5941 ggccaatcat atgcctgcag ggccgcgagc gtatcggcgg acagttcgcg cgactgaaca
     6001 ccagcgttct cggccgagcg cacaatgaaa tgctgcgaca gcgccgggac atcctcgcga
     6061 cgctccttca gagcgggtac cttcaacggg acgacggcga gccggtaata gagatcctcg
     6121 cggaaccggc cagaagcgat ctctgtctga aggtctttgt tggtggtgga aagcacgcga
     6181 acatcgacct ttatccgcgc tgaaccgccc aggcgctcga aggtcgaatc ctgcagcgca
     6241 cggacgatct tgccctgcgt ctcgagcggc atgtcggaga cttcgtccaa tagcagcgtg
     6301 ccgccatgcg cacgctccaa cacgccggcg cgacgcggtt gcgcgatcgg atcagcgccc
     6361 gcttccagac cgaacagctc ttcctcgaac cggccgggcg cgagcgtggc acaattcaga
     6421 acaatgaacg gtccctcggc acgacgggac cgcgcgtgga tcatccgcgc gacaacttcc
     6481 ttgccgctac cggcaggccc gtggataagc gcgcgcgaac cggtcggcgc cacgcgctcg
     6541 acgccggtgc gcaatacgcc aatagccgaa ctgtcgcccg tcagggatgt ttcggggccg
     6601 gcgcgcagac ggagttcagc attctctcgc gccaatgcgg cggcttccag ggcgcggcgc
     6661 accacgagaa gcaggcgatc tgacttgaac ggcttctcga tgaagtcgta ggcgccttgc
     6721 tggatagcgg tcaccgccat ctcgatgttg ccatggccag agatcatcac gacgggaaca
     6781 tggggttcct cgctgtggaa tgcctccagg ataccgaggc catccatacg cgaaccttgc
     6841 agccagacat caagaatggc cagcgacgga cggcgcatgc ggaacgcggc gattgctgcc
     6901 tcggagtccg cggcgccacg cgtctcgtag ccttcgtcgc gcagcacgcc atcgatgagc
     6961 atacggatat cgggttcgtc atcgacgatc aggacgtcat acgccatccg gcacctttat
     7021 gggcaatacg agcgtggcca ccgctccagg accgtcagtg cgatcttcga gtgtcagccg
     7081 tccgccgtgg tcttccatga tcttcttcac gatggcgagg cccagccctg tccctttcgg
     7141 cttatgagtt acataaggtt cagtgagccg cgcacgatct tcctgcggca atccgacacc
     7201 atcatcggtc acgatgatgc gaacgtcgct ctgatcgcat tgcacgcaga ggccgatatg
     7261 gccggcgctt tcgcgcatcg cgacggcatc ggcggcgttc tgcaacagat tggtcagcgc
     7321 ctgcccaatg agccgccggt cgcagggagc gactggtcct ttttcgggga tttcggtgca
     7381 ccagtcgatc tgcggacgtg cggtcttttg cagcaccagc atctcacgcg cgacgcgacc
     7441 gacatcctcg gacttgatga ccgggtgtgg catgcgcgcg aaggccgaga actcatccac
     7501 catgcggccg atatcgccga catggcggat gatcgtatcg gcgcattgct tgaacgtgtc
     7561 aggatccgtg gtgatttcct tggcaaagcg acgcttcagc cgttccgctg cgagttgtat
     7621 cggcgtcagc gggttcttga tttcgtgtgc gatgcggcgc gccacatcgg cccaggcggc
     7681 cttgcgctgc gcggattgca gttcagtgat gtcgtcgaat gtgacgacga aaccatcagt
     7741 ccgtccgccg gacagatcgg cgccgatacg caccagcagc gtgcggcggc gcgtcgccgg
     7801 cccaat
//
LOCUS       DATGEM010000689         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1309003, whole genome shotgun sequence.
ACCESSION   DATGEM010000689 DATGEM010000000
VERSION     DATGEM010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309003"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VKQ27_21225"
     CDS             <1..1218
                     /locus_tag="VKQ27_21225"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HLY91519.1"
                     /translation="LNFLGCALGVAHTPPIEMALRVLLPLSGEDRVTVLGRKELLDPL
                     GAAFINAVGANLLDYDDTHLRTVIHPSAPVAPAALALAEQRGLSGAAMLHAFILGAEV
                     ECRIGNAVSPGHYARGWHITSTCGVFGAAAAAAKLLGLDAMQTWHALGIAASQSGGII
                     ENLPSAAKNVSVGNAARNGLFAALLAEQGYTAAPAAIEGALGWARAMGDEPDVAEITG
                     GLGERWELLSNTYKPYPCGIVMHSVIDACLELRQQVAPDEIARIVISGEQLLLDRGDR
                     IVTNERDARVSIHHCAAVAFLFGAAGLEEFSEAAVHDPAVVALRAKTEARVDAASPRG
                     AATAEVFTHDGRHLRNTVLHAKGSRERPLSDEEIEVKVRQQAAIARIDDVIAAAWRID
                     MLADVGTLLCASC"
     gene            1203..1937
                     /locus_tag="VKQ27_21230"
     CDS             1203..1937
                     /locus_tag="VKQ27_21230"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLY91520.1"
                     /translation="MRILLINPNTTEAVTQRMAEAARNVAAPDVVIETATARFGPQII
                     GSRVESAVAAAAVVERLSQAEGYDAVIVGASVDPGLTAAREMLDVPVIGITEAALHAA
                     CLLGGKIGAIVMSANSDTILREMADAYGLGTRLGAVRTLPITPLELLADLDAATRAIA
                     AAADAMVADDGVDTIALIGAVMAGMAPVVQPAVRVPVLEGVSAAVGLAEALVRLRVSP
                     SRAGSYAKPPGERVALLARALGKGPG"
     gene            2031..>2706
                     /locus_tag="VKQ27_21235"
     CDS             2031..>2706
                     /locus_tag="VKQ27_21235"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY91521.1"
                     /translation="MAGKIRVGMVGITPNRGFSSIAHMPALQGLPEFEVIAVCTTRQE
                     SAEAAARHYGVPLAFADPVKLAQHPDVDMVTVCVKVPDHLTPVMAAIDAGKHVYSEWP
                     LGRTTAEAMQMRDAAERKGVRHAVGLQGQVSPSINYVKDLIAEGYVGTPLSATLFVNA
                     ANWGALLDRSYQADFGNGANLMTITGGHNLDALCYCLGEFRELSAFAVSQRAEIPTQD
                     SGIVAKD"
BASE COUNT          443 a          801 c          987 g          475 t
ORIGIN      
        1 ctgaactttc tcggctgcgc gctgggcgtt gcgcatacac cgccgataga gatggcactg
       61 cgcgtgctgt tgcctttgtc cggcgaggac cgtgtcacgg tgctgggccg gaaggaactt
      121 ctcgatccgc ttggcgccgc attcatcaac gctgttggcg ccaacctgct ggactatgac
      181 gatacgcatc tgcggacggt gatccatcca tcggctccgg tggcgccggc agcacttgcg
      241 ctggcggaac agcgcggact gtccggcgcg gccatgctgc atgcgttcat tctcggcgca
      301 gaggtcgaat gccgcatcgg caatgcggtt tcgcccgggc actatgcgcg cggctggcat
      361 atcacttcga cctgcggcgt gttcggcgcg gccgcggcgg cggcgaagct gcttgggctc
      421 gatgcgatgc agacctggca cgcgctcggc atcgctgcca gccagtcggg gggcatcatc
      481 gagaacctgc ccagcgcggc gaagaacgtc agcgtgggga atgcagcgcg taacgggctg
      541 ttcgcggcgc tgttggcgga gcagggctat acggcggcac cggcggcgat cgaaggtgcg
      601 ctcggctggg cgcgtgcgat gggcgacgaa ccggacgttg cagagatcac cggtggtctc
      661 ggcgaacgct gggaactgct gagcaacacc tataagccct atccatgcgg catcgtgatg
      721 cacagcgtga tcgatgcctg cctggaactg cggcagcagg tcgcaccgga cgagatcgcg
      781 cggattgtca tttccggcga gcagctgctg ctggatcggg gcgatcggat tgtgacgaac
      841 gagcgcgatg cgcgggtcag catccatcac tgcgccgccg tggccttcct gttcggtgcc
      901 gctggcctgg aggaattctc cgaggccgcc gtgcacgatc ctgctgtcgt cgcgctgcgc
      961 gcgaagaccg aggcacgcgt ggacgctgcc agtccgcgag gtgcggccac ggccgaggtg
     1021 ttcacgcatg atggacgaca tttgcgaaac actgtgctgc atgccaaggg cagcagggag
     1081 cggccgctgt cggatgagga gatcgaggtg aaggtgcgac aacaggctgc tattgcgcgc
     1141 atcgacgatg tgatcgccgc ggcgtggcgg atcgatatgc tggcagatgt cgggacattg
     1201 ctgtgcgcat cctgctgatc aatcccaaca cgaccgaggc ggtcacgcag cgcatggccg
     1261 aggccgcgcg gaacgtcgct gcgccggacg tggtgattga gacggcaacg gcgcggtttg
     1321 gcccacagat tataggcagc cgggtggaaa gtgctgtcgc cgccgccgcg gtggtggagc
     1381 gattgtcgca ggccgagggc tatgatgccg tgatcgtcgg cgcgtcggtc gatcccggcc
     1441 tgacagccgc gcgcgaaatg ctggatgtcc cggtgatcgg cataaccgag gcagcgctgc
     1501 acgccgcctg cctgcttggc ggcaagattg gcgcgatcgt catgtcggcc aacagcgata
     1561 cgatcctgcg cgagatggcg gatgcatatg ggctggggac acggctcggc gccgtgcgga
     1621 cgctgccgat cacgccgctg gagctgctgg ccgatctgga tgcggcgacg cgcgccatcg
     1681 cggcggcggc cgatgccatg gtagcggacg atggtgtcga tacgatcgca ttgattggcg
     1741 cggtgatggc aggcatggcg ccggtcgtgc agcccgctgt gcgcgtgccg gtactggaag
     1801 gcgtcagcgc tgcggtcggg cttgccgagg cactggtgcg gctgcgcgtg tcgccatcgc
     1861 gtgctggcag ttacgcgaaa ccgccagggg agcgcgtagc gttgctggca cgcgcgcttg
     1921 ggaaggggcc gggctgatgg cagcgtgtca ttgcgtgtgc ggcgaagcaa tcctggtcga
     1981 gattgcttcg ctgcgctcgc aatgacagtc tgtcctggga ggcaaacgtc atggcaggca
     2041 agattcgtgt gggcatggtc gggatcacgc cgaaccgtgg gttttcttcc atcgcacata
     2101 tgccggcact gcaagggctg ccggagttcg aagtgatcgc agtctgcacg acacggcagg
     2161 agagcgccga ggccgcggcg cggcactacg gtgtgccgct cgcgttcgcc gatccggtga
     2221 agctggcgca gcatccggat gtggatatgg tgacggtgtg cgtgaaggtg ccggatcacc
     2281 tcacgccggt gatggcggcg atcgatgccg gcaagcacgt ctattccgaa tggccgctcg
     2341 gccgcacaac ggccgaggcg atgcagatgc gcgacgcggc cgagcgcaag ggcgtccgcc
     2401 acgcggtggg tctgcagggc caggtgtcgc cctcgatcaa ctacgtgaag gacctgatcg
     2461 ccgaggggta tgtaggcacg ccgctcagtg ccacgctgtt cgtcaatgcg gcgaactggg
     2521 gggcgttgct ggatcgctcc tatcaggcgg acttcggcaa cggcgccaat ctgatgacga
     2581 tcaccggcgg gcacaacctc gatgcgctgt gctattgcct gggcgagttc cgcgaactgt
     2641 ccgcgttcgc cgtcagccag cgcgcagaaa ttcccacaca ggattccggc atcgtcgcca
     2701 aggatg
//
LOCUS       DATGEM010000690         6941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1205722, whole genome shotgun sequence.
ACCESSION   DATGEM010000690 DATGEM010000000
VERSION     DATGEM010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205722"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VKQ27_21240"
     CDS             <1..1026
                     /locus_tag="VKQ27_21240"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLY91522.1"
                     /translation="SGSEANEAGFKIARQYQKHEKPGEFRYKTVSRYYAYHGTTLATL
                     DAGGMGDRKSKFEPYSGDFVHVAPPYCYRCPFGLSYPSCGMACVKNMESAILGENPDT
                     VAEVIVEPIMSGIGVAVPPDEYLKEVETICRRYGILLHVDEVINGFGRTGKMFAHQHY
                     GVSPDIIAVAKGISSAYMPIAATVVKNSVFESFYGEPDQNRQVAQVNTYGGHPVAAAV
                     ALRNIEIMEAEKLADRAAEIGTYLQDGLRTLLKQPLVGDVRGKGLLQAVELVKDRATK
                     EPVDASVITKVVDFCRDQGVIVGRAGGGRRFGNVIVLSPPLVITRAECDRLVDTLDRA
                     LAALPVG"
     gene            1167..3134
                     /gene="parE"
                     /locus_tag="VKQ27_21245"
     CDS             1167..3134
                     /gene="parE"
                     /locus_tag="VKQ27_21245"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HLY91523.1"
                     /translation="MNDLFSGKGSDGYSAKDIEVLEGLEPVRRRPGMYIGGTDETALH
                     HLAAELLDNAMDEAVAGHASFIEVTLEAGNWLTVRDNGRGIPVDPHPKFKNLSALEVI
                     LTTLHSGGKFGGKVYSTSGGLHGVGSSVVNALSEKLEVEVARDRVLWKQSYARGKPAT
                     KLVNGGAVHNRRGTSIRFKPDSTIFGAVSFSPARLYRLCRSKAYLFRGVQIRWACDPS
                     LLKGSKEDTPAEAVLHFPGGLRDSLEAEVGEAPRVLPQVWSGEADLPGGSGRVEWAGA
                     WLEDGEGFVHSYCNTIPTTLGGTHEAGFRAALLKGLRAWGEQRGNRRAAQMVAEDLLG
                     TLAAKLSVFLRDPQFQGQTKERLTSPDATRLVETALRDRFDHWLAGDPAQADNLLAFV
                     IERAEERMRRKETKDVARKSATRKLRLPGKLTDCTRENAAETEIFLVEGDSAGGSAKQ
                     ARNRETQAVLPLRGKILNVASASADKLRQNQELKDLIEALGCGVGDRFDRARLRYGRV
                     IIMTDADVDGAHIASLLMTFFYRELPELVRHGHIYLAQPPLYRLTQGAKSIYAMDDAD
                     REAKVKREFKAGAKVEVSRFKGLGEMPPSQLKETTMDPAKRTLLKVVALPEERAKNNE
                     LVESLMGRRPELRFQFIQERAKTVEVEEVDV"
     gene            3206..3781
                     /locus_tag="VKQ27_21250"
     CDS             3206..3781
                     /locus_tag="VKQ27_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91524.1"
                     /translation="MTHPVIVVFTAKPLEEIVRERASRDWRLDAERARQAEFLVCTQN
                     QHNQGFRAPAAPHGSAFLIGRIAGVVPSWERPDRWAITISDYLPLNPTIPGIWGKSGN
                     LRYPVWYTTLEELGIELDRLPAFTHLSRLADGMAEMQVRPIAASIPWTRGADDAGAWR
                     RLDAILAQLDRVPDLPAGREPLEWDEHGLPR"
     gene            3778..4176
                     /locus_tag="VKQ27_21255"
     CDS             3778..4176
                     /locus_tag="VKQ27_21255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLY91525.1"
                     /translation="MIAVDASAIVAIVFGEPERAAFREAILQAGRALISTASVLEVKM
                     VVFGRRGPRAVVLVDDLLRLPMFEIVPSGPAELEAAFNAFVVFGKGSGHSASLNYGDL
                     FSYALAKVRDLPLLFKGTNFAETDILPAVT"
     gene            4188..5000
                     /locus_tag="VKQ27_21260"
     CDS             4188..5000
                     /locus_tag="VKQ27_21260"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY91526.1"
                     /translation="MTAPFLAELRRRSGAAVDPERFPWSLPLVRDLETLSFTTPITFL
                     VGENGSGKSTLLEGLAVGLDAVAAGAHDLQRDPSLQPARDFAAGFVFARRRHARTRLF
                     LRAEDVFGYTGRVTADIAELGAIEQDFASLPEGPGRERAMGMARGERAALTRRYGENP
                     DGRSHGETFLALLQSRLVPNGLYLLDEPETPLSPSRVLALISLLAGCVKRECQFVIAT
                     HSPLLMAMPGATILLAEDGRLRPVEWDDLEHVRITRAFMRDPGAVLRRLLDE"
     gene            5120..6253
                     /locus_tag="VKQ27_21265"
     CDS             5120..6253
                     /locus_tag="VKQ27_21265"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HLY91527.1"
                     /translation="MAIAAKPDVRPANPNFSSGPCAKRPGFSLNALAGAMLGRSHRAA
                     APKARLAEVIERSRTILGMPADWRLGIVPASDTGAMEMALWSLLGARGVDVVAFESFG
                     EGWATDVVKQLRLPDARVLSAPYGQLPDLGAVDFARDVVFPWNGTTSGVRVPNGDWIA
                     GDRQGLTICDATSAAFAMRLDWQKLDVVTWSWQKVLGGEAAHGMLALSPRAVERLLSY
                     KPDWPLPKVFRLTSGGKLIEGIFKGETINTPSMLCVEDALDGLKWAESVGGLDGLIVR
                     SEANLAAVARWVERTPWADFLAEDPATRSCTSICLKAPKQVASVLEKEGAAYDIASYR
                     DAPAGLRIWGGATVDTADIEALLPWLDWAHGAANGTAQPKAAE"
     gene            6264..>6941
                     /locus_tag="VKQ27_21270"
     CDS             6264..>6941
                     /locus_tag="VKQ27_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HLY91528.1"
                     /translation="MPKVLISDKLSPAAVDIFRRRGIDVDLKPGLSPADLRAIIAPYD
                     GLAVRSATKVTRELLDAATNLKVVGRAGIGVDNVDVRSATARGVVVMNTPHGNTITTA
                     EHAIALMFALARQIPEASASTKAGKWEKNRFMGVELTGKTLGLIGCGNIGSIVADRAL
                     GLRMRVISYDPFLNEKRALELGVEKVELDDLLARADIITVHTPLTDTTRNILSRDALA
                     KTQRGVRI"
BASE COUNT         1233 a         2042 c         2403 g         1263 t
ORIGIN      
        1 tcaggctccg aggcgaacga ggcaggcttc aagatcgcgc gccagtacca gaagcacgag
       61 aagccgggcg agttccgcta caaaaccgtc agtcgctact acgcctatca cggcaccacg
      121 ctggccacgc tggatgccgg cggcatgggc gaccgcaagt cgaagttcga gccgtattcc
      181 ggcgatttcg tgcacgtggc gccgccctac tgctaccgct gcccgttcgg gctgagctat
      241 ccgagctgcg gcatggcgtg cgtcaagaac atggaaagcg ccatcctcgg cgagaacccg
      301 gatacggtgg ccgaggtcat cgtcgagccg atcatgagcg gtatcggcgt cgctgtgcct
      361 cccgatgagt acctgaagga ggtcgagacg atctgccgtc gctacggcat cctgttgcac
      421 gtcgatgagg tgatcaacgg gttcggccgg acgggcaaga tgttcgcgca ccagcactac
      481 ggcgtgtccc cagatatcat cgcggtggcc aagggcatct cgtccgccta catgccgatc
      541 gcagcgaccg tggtgaagaa ctcggtgttc gagagcttct atggcgagcc tgatcagaac
      601 cgccaggtcg cgcaggtgaa cacctatggt ggccatccgg tggccgctgc cgtggcactc
      661 cgcaacatcg agatcatgga agccgagaaa cttgccgacc gcgccgccga gattggcacg
      721 tatctgcagg atggtctgcg tacgctcctg aagcagccgc tggtgggcga cgtgcgcggc
      781 aagggcctgt tgcaggcagt cgagctggtg aaggaccgcg cgaccaaaga accggtggat
      841 gcctcggtga tcaccaaggt ggtggatttc tgccgcgatc agggggtcat cgtcggccgc
      901 gccggtggcg gccgccgctt cggcaatgtg attgtgctgt caccgccact ggtgatcacg
      961 cgggcggagt gcgaccggct ggtcgatacg ctggatcgcg cgctggcggc gctgccggta
     1021 ggctagccca cccgcgcacg acgccgcgtg gcgatcgctc ccgcacgtcc ctggcacctg
     1081 gtccagctgg gctggcagcc ggccgatcgg gttgccctga ctcctccggt ccggctagct
     1141 tgccgtgggt tggatgagaa cacagcatga acgatctttt cagtggcaag ggatctgacg
     1201 gatattcagc caaggacatc gaggtccttg aaggactgga gccggtccgg cgtcggcccg
     1261 gcatgtatat cggcggcacc gacgaaacgg cgctgcatca cctggccgcg gaactcctgg
     1321 acaacgccat ggacgaagcg gtggccggcc atgccagctt catcgaggtg actctggaag
     1381 caggtaactg gctgacggta cgcgacaatg gtcgtggcat tccggtggac ccgcatccga
     1441 aattcaagaa tctcagcgcc ttggaagtga tcctaacgac gctgcattca ggcgggaagt
     1501 tcggtggcaa ggtatattca acttcgggtg ggctgcatgg tgtcggcagt tcagtagtga
     1561 acgcgctgtc agagaagctg gaggttgagg tcgcgcgcga ccgggttctg tggaagcaga
     1621 gctacgcgcg gggcaagccg gcgaccaagc tggtgaacgg cggggcggtg cataaccgtc
     1681 gcgggacttc gatccgcttc aagcctgatt ctacgatatt tggtgccgta tcgttcagtc
     1741 cggcgcggct ctatcggctg tgccgttcga aggcctatct gttccgcggc gtgcagattc
     1801 gctgggcgtg cgatccatct ctcctgaagg gcagcaagga agatacgccg gccgaggcgg
     1861 tgctgcattt tcccggcgga ctgcgcgaca gcctggaggc ggaagtcggc gaggcgccgc
     1921 gcgtgctgcc tcaggtttgg tccggcgaag ccgatctgcc gggcggatcc ggccgcgtgg
     1981 aatgggcagg ggcgtggctg gaggacgggg aagggtttgt ccactcctac tgcaacacca
     2041 ttccaacgac cctgggtggc acgcacgagg cgggctttcg cgcagcgctg ctgaaggggc
     2101 tgcgtgcctg gggtgagcaa cgcggcaatc gccgggcggc gcaaatggtg gcggaggatc
     2161 tgctgggcac gcttgcggcg aagctctcgg tcttcctgcg cgatccgcaa ttccaggggc
     2221 agacgaagga aaggctcacc agcccggatg cgacacggct ggtggagacg gcgctgcgtg
     2281 accggttcga ccactggctc gcgggcgatc ctgcccaggc agacaatctg ctcgcctttg
     2341 tcatcgagcg tgccgaagag cggatgcgcc ggaaggaaac gaaggacgtc gcgcgcaaat
     2401 ccgccacgcg gaagctgcgg ctgccgggga agctgaccga ctgcacgcga gaaaatgctg
     2461 cggaaaccga gatcttcctg gtggagggtg acagcgccgg cgggtccgca aagcaggcgc
     2521 gcaatcgcga gacccaggct gtgctgccac tgcgcggcaa gatcctgaac gtcgcatccg
     2581 cttcggctga caagctgcgc cagaaccagg aactgaagga tctgatcgag gcgttgggct
     2641 gcggcgttgg cgaccggttc gatcgcgcca ggctgcgcta cgggcgcgtc atcatcatga
     2701 ccgatgccga cgtggacggg gcgcatatcg cctcgctgct gatgacgttc ttctaccggg
     2761 aactgcccga actggttcgg catgggcata tctatctggc gcaaccgccg ctctaccggc
     2821 tgacccaggg ggcgaagtcc atttacgcga tggatgatgc tgatcgcgag gcgaaggtga
     2881 agcgtgagtt caaggcgggg gcaaaggtgg aagtcagccg gttcaaaggg ctgggcgaga
     2941 tgccaccatc ccagttgaag gaaaccacga tggatccggc aaagcgcacg ctgctgaaag
     3001 tggtggcgct gccggaggag cgggcgaaga acaatgagct ggtcgaaagc ctgatgggac
     3061 ggcggccgga actgcggttc cagttcatcc aggagcgcgc gaagactgtg gaggtggagg
     3121 aggtggatgt ttgacatcta ccatcattga attccttcga tgtttgtgat attaagatca
     3181 tcatattctg tcgaaggggt ggcacatgac ccatcctgtt atcgtggtat ttaccgcaaa
     3241 gccgctcgaa gagatcgtcc gggagcgggc ctctcgtgat tggcggcttg atgccgagcg
     3301 ggcgcggcag gcggagttcc tggtctgcac gcaaaaccag cataatcaag gatttcgggc
     3361 tccggccgcg ccgcacggct ccgccttcct gatcggtcgg atcgccggcg tcgtgccgtc
     3421 ctgggagcgc ccggaccgtt gggcaatcac catcagcgat tatctcccgc taaatccgac
     3481 aattccagga atttggggaa aatccggaaa cttacgctat ccagtctggt acaccacgct
     3541 cgaggagctt ggcatcgaac tggatcggct gcctgccttt acccatctgt cccgcttggc
     3601 ggatgggatg gccgaaatgc aggtacgccc aatcgcggca tcaatcccct ggacgcgggg
     3661 cgccgacgat gccggtgcgt ggcggcggct ggatgctatc ctggcgcagt tggatcgcgt
     3721 gccggacttg ccggcagggc gagagcctct cgaatgggat gagcacggcc tgccacgatg
     3781 atcgcggtag atgcctcggc gatcgtcgcg atcgttttcg gggaaccgga acgcgcggcg
     3841 tttcgtgagg cgatcctgca ggcgggaagg gcgctgatca gcacggcctc ggtgctggag
     3901 gtgaagatgg tggtctttgg gcgacgcggt cctcgcgctg tggtactggt ggacgacctg
     3961 ttgcggctgc ccatgttcga gatcgtcccg tcaggcccgg ccgaacttga ggctgcgttc
     4021 aacgcattcg tcgtgttcgg gaagggcagc ggccattcgg catcgttgaa ctacggcgat
     4081 ctgttctcct acgcactcgc aaaggtgcga gacctgccgc tgctcttcaa aggcaccaat
     4141 ttcgccgaga ccgacatctt gccggccgtt acctgactgg cctcggtatg acggcaccat
     4201 tcctggctga attgcgccgc cggtctggtg cggcagtcga tccagagcgc ttcccatggt
     4261 cgctgccgct ggtgcgcgac ctcgagacgc tctcgttcac gacacccatc acatttcttg
     4321 tgggcgagaa tggcagcggc aagtccacgc tgctggaagg gcttgcggtc ggcctcgatg
     4381 cggtggcggc gggagcacat gacctccagc gagatccaag cttacagccg gcgcgcgact
     4441 tcgcggcggg gttcgtcttc gcacggcgca ggcacgcccg cacgaggctg ttcctgcgcg
     4501 ccgaggacgt attcggctac accggccgcg tgaccgcaga catcgccgag ttgggcgcga
     4561 tcgaacagga ttttgcctcg ctgccggaag gtcccggacg tgagcgcgcg atgggcatgg
     4621 cccgtggaga gcgggccgca ctcacacggc gctatggcga gaacccagac ggcaggtcgc
     4681 atggcgaaac atttctggcc ttgctgcaat cgcggctggt accgaatggg ctgtatctcc
     4741 tcgatgagcc tgaaaccccg ctttcaccct cgcgcgtact ggcgctgatc tcgttgctcg
     4801 cggggtgcgt caagcgagaa tgccagttcg tgatcgcaac gcactcgccg ctgctgatgg
     4861 ctatgccagg cgcaaccata ctcctcgccg aggacggtcg tttgcgtccg gtggaatggg
     4921 acgatctgga acacgtgcgg atcacccgcg ccttcatgcg ggacccgggc gcggtgctgc
     4981 gtcgcctgct ggacgagtag aacaatataa tcgtgccctt tgcttgcggt cgttgacgcg
     5041 acgtcaatgc tttcgctata cgccggcgcg ggtcacgccc ccccaccggg gcgcttctct
     5101 tctgtaaggg agagccgcaa tggcaatcgc cgcgaaaccg gacgtgcgtc cggccaatcc
     5161 gaatttctcc tctggcccgt gtgccaagcg tcccggcttt tcgctgaacg cgttggctgg
     5221 tgcgatgctc gggcgcagtc atcgcgcagc cgcgccgaag gctcgactgg ctgaggtgat
     5281 cgagcgatca cgtacgatcc tcggcatgcc ggctgactgg cggctgggca ttgtgccggc
     5341 ctccgacact ggcgcgatgg agatggcgct gtggtcgctg ctcggcgcgc gcggtgtcga
     5401 tgtcgttgca tttgaaagtt ttggcgaagg ctgggcgacc gacgtggtga agcagcttcg
     5461 gctgccggat gcgcgtgtgc tttcggcgcc gtacgggcag ttgccggacc ttggcgccgt
     5521 ggatttcgcg cgcgatgtgg tctttccgtg gaatggcacg acgtcgggcg tgcgcgtgcc
     5581 gaatggagac tggatcgcgg gcgatcggca gggtctgacg atatgtgatg cgacctcggc
     5641 ggcgttcgcc atgcggctgg actggcaaaa gctcgatgtg gtgacgtggt cctggcagaa
     5701 ggtgctggga ggtgaggcag cgcacgggat gctcgcgctg tcaccgcgcg cggtggagcg
     5761 gctgctcagc tacaagcccg actggccgct gccgaaagtg ttccggctga cctcgggcgg
     5821 caagctgatc gagggcatct tcaagggcga aactatcaac acgccgtcga tgctgtgcgt
     5881 cgaggatgca ctggatgggc tgaagtgggc agagagcgtg ggcggtctgg atgggctgat
     5941 cgtgcgatcg gaagcgaacc tggcggcggt ggcgcgatgg gtcgagcgca caccgtgggc
     6001 ggacttcctc gctgaggatc cggcgacgcg ctcttgcacc tcgatctgcc tgaaggcgcc
     6061 gaagcaggtc gcgagtgtgc tcgagaagga gggtgccgca tacgacatcg cctcgtatcg
     6121 cgatgcgccg gcgggcttgc ggatctgggg cggagcgacg gtcgataccg cggacatcga
     6181 ggcattgctt ccgtggctgg actgggcaca cggcgcggcg aacggaacgg ctcagcccaa
     6241 ggcagcggaa taggcacagc gatatgccca aggtattgat cagcgacaaa ctctctccag
     6301 ccgcggtgga catcttccgc cggcgcggca tcgacgttga cctgaagccg ggcctctcgc
     6361 cggccgatct gcgcgcgatt atcgcgccgt atgacgggct ggcggttcgc tcggccacca
     6421 aggtcacgcg cgaactgctg gatgcggcga ccaacctgaa ggtggtgggc cgtgccggca
     6481 tcggcgtgga caatgtcgat gtccgttcgg caacggcgcg tggcgtggtg gtgatgaaca
     6541 caccgcacgg caacaccatc accaccgctg aacacgccat cgccctgatg ttcgcgctgg
     6601 cgcggcagat cccggaggcc tctgcatcaa ccaaagccgg caagtgggaa aagaaccgct
     6661 tcatgggtgt cgaactaacc ggcaagacgc tcggtctgat cggctgcggc aatatcggct
     6721 cgatcgtcgc cgatcgcgcg ttggggctgc ggatgagggt gatctcgtac gatcccttcc
     6781 tcaacgaaaa gcgcgcgctg gagttgggtg tcgagaaggt ggagctcgac gatctgctgg
     6841 cgcgcgccga catcatcact gtccacacgc cgctgacgga cacgacacgc aacatcctct
     6901 cgcgcgatgc gttggcgaag acgcagcgtg gcgtgcgtat c
//
LOCUS       DATGEM010000691         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_604891, whole genome shotgun sequence.
ACCESSION   DATGEM010000691 DATGEM010000000
VERSION     DATGEM010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604891"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..608
                     /locus_tag="VKQ27_21275"
     CDS             207..608
                     /locus_tag="VKQ27_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HLY91529.1"
                     /translation="MATGIRGKCPRCGQGHLFDGFLTLRDSCDVCGLPYSFADPADGP
                     AFFVICFGCVPSVVFALMLQILYEPPFWVHLVTSLPVLLATCLAPLRPLKGWLVCSQY
                     FYQAREGRLAAPWSPFTGGAVIPMPPQSQSR"
     gene            618..917
                     /locus_tag="VKQ27_21280"
     CDS             618..917
                     /locus_tag="VKQ27_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91530.1"
                     /translation="MLDQARLLAADFLVQHGQEADARIVRQGGGDDFPEVRLALALQK
                     THAAQLQRYERALRTYADGAFWDAEIAEAALAYHDQGAVARAALDGKELFALHRD"
     gene            complement(921..1817)
                     /gene="cynR"
                     /locus_tag="VKQ27_21285"
     CDS             complement(921..1817)
                     /gene="cynR"
                     /locus_tag="VKQ27_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HLY91531.1"
                     /translation="MLLRHIRYLMAVADEGSFTRAALALHVSQPALSQQIRQLEERMG
                     VALLDRSGRMVRPTDAGEAFLAYARRAMGELEAGSRAVRDVADLSTGALRIGFTPSFS
                     AYLVAPLVRCFHERFPGILLSVVEMAQEEMEPALGADALDLGIAFSDVRAEDVEWLPL
                     HEERLSFVVGEAHPASGAESMDCAALAHEELALLGVTFATRLTVDAYLHRQHVRPRIA
                     VEASSIAAIVEIVRSTRLGTILPDAVACEQPGIRAVALVPPIETRRVVLLQRRGGYRS
                     AAARAFTALAEEYAAEVGGGVV"
     gene            1920..2570
                     /locus_tag="VKQ27_21290"
     CDS             1920..2570
                     /locus_tag="VKQ27_21290"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLY91532.1"
                     /translation="MEELIAGFRKFREEAFPAREGLFQQLAHSQTPGTLFVTCSDSRV
                     VPELLTQREPGDMFVVRNAGNIVPSYGPEPGGVSATVEYAVAALGVRDVVICGHSDCG
                     AMTAVAHDHDLHHMPAVCSWLRHADAAKAVNNAHDHGSDAAKLDGMVRENVIAQIANL
                     RTHPSVALALAQGKLTLHGWVYDIESGAIDAIDGSTGRFVPLTENPDASATGAPRL"
     gene            2675..3145
                     /gene="cynS"
                     /locus_tag="VKQ27_21295"
     CDS             2675..3145
                     /gene="cynS"
                     /locus_tag="VKQ27_21295"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016738501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HLY91533.1"
                     /translation="MLQHQVSQSARQNLTDVVIAAKAAKDLSWKKIAEGTGFAKEFVT
                     AALLGQHLLPREAATLVGERLGLDAEQVHLLEAVPLRGSFPDGVPTDPTLYRFYEMIQ
                     VYGTTLKALVHEEFGDGIISAINFKLKVEKVEDPDGGHRAVITLDGKYLPTKPY"
     gene            complement(3195..>3444)
                     /locus_tag="VKQ27_21300"
     CDS             complement(3195..>3444)
                     /locus_tag="VKQ27_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91534.1"
                     /translation="DPHGHHLWLRLPEHWRATDFADHADRLGVAIVPSTVFSISPSPV
                     EAVRVSLGVAPDRAALEDALLVLSGLLEQPSLAVRAVV"
BASE COUNT          631 a         1221 c         1036 g          556 t
ORIGIN      
        1 cgccacatgg agcaatcgct taaggcgcgc gcctcctcgg gcgatcccgc ctatcggccg
       61 atcgacaatc cttgacattg tatggccata tgccatatac gacgcataca ctgcttacat
      121 tgtccataca gagcgtgccc aatcccgcac ggcaaggaca ggctatgacc gaacactccg
      181 accgcacatg gccgaggctg gagccggtgg ccaccggcat tcgcggcaaa tgcccacgct
      241 gtgggcaagg ccacctcttc gacggcttcc tcacgctgcg cgactcctgc gatgtgtgcg
      301 gcctgcccta cagcttcgcc gatccggcgg acggcccggc cttcttcgtg atctgcttcg
      361 gctgtgtgcc gagcgtggtc ttcgcgctga tgcttcaaat cctctacgag ccgcctttct
      421 gggtgcatct ggtcaccagc ctgcccgtgc tgctcgccac ctgcctggcg ccgctgcgcc
      481 cgctcaaggg atggctggtg tgcagccagt atttctatca ggcgcgcgaa ggtcggctgg
      541 cggccccctg gtcgccgttc accggcgggg cggtcattcc gatgccgccg caatcgcaga
      601 gccgttgagg gccggccatg ctcgatcagg cacgcctgct cgcagcggac ttcctcgtcc
      661 agcacgggca ggaggcggac gcgaggatcg tgcgtcaggg tggcggcgac gacttccccg
      721 aggtgcggct cgctctggcg ttgcaaaaga cgcatgctgc ccagctccag cgctacgagc
      781 gcgcgttgcg gacgtatgcc gatggcgcct tctgggatgc cgagatcgcc gaggcggcgc
      841 tcgcctatca cgatcaaggc gcggtggcgc gggcggcgct ggacggcaag gaattgttcg
      901 ccctgcaccg cgactgacgc ctacaccacg ccgccgccga cctccgccgc atattcctcc
      961 gccagggcgg tgaacgcccg cgccgccgcg ctgcgataac cgccgcggcg ctggagcagc
     1021 accacccgcc gcgtctcgat cggtggcacc agcgccaccg cccggatccc cggctgctca
     1081 caggctaccg catccggcag gatggtcccg agccgggtgc ttcgaacaat ctcgacaatc
     1141 gccgcgatcg aactcgcctc caccgcgata cgggggcgga catgttggcg atgcagatag
     1201 gcatcgacgg tcagtcgtgt cgcgaacgtc acgcccaaca gggcgagttc ctcatgtgcc
     1261 aacgccgcac aatccatcga ctccgcgccg ctggcgggat gcgcttctcc caccacgaac
     1321 gacaatcgct cctcgtgcag tggcagccat tcgacgtcct ccgcacgcac gtcactgaac
     1381 gcgataccga gatcgagcgc gtcggcgccg agcgccggct ccatctcctc ctgcgccatc
     1441 tcgacaacgc tcaacaggat gccgggaaaa cgctcgtgga agcatcgaac cagcggtgcg
     1501 accaggtagg cgctgaagct cggcgtaaag ccaatccgca atgccccggt cgaaagatcg
     1561 gcgacgtcgc gcaccgcccg gctccctgcc tccagttcgc ccatcgcgcg gcgggcatat
     1621 gcgagaaagg cctcgcccgc gtcggtcggt cgcaccatcc gcccgctccg atcgagcaac
     1681 gccacgccca tccgctcctc gagctggcga atctgctgcg acagcgcggg ctgcgacacg
     1741 tgcagcgcca gcgccgcgcg ggtgaaactg ccctcatcgg caacagccat gaggtagcga
     1801 atatgccgca acagcatagt tataagccct gcttatcgca tctgtaagaa cacggtctta
     1861 gacaattata cctgcaaagt ccaaatccgt ctcaacagat tgaagacgga gataacgcca
     1921 tggaagaatt gatcgcaggg ttccgcaagt tccgggaaga agcttttccc gctcgcgaag
     1981 gcctttttca gcagctcgcc cacagccaga cgcccggcac cctgttcgtg acctgctcgg
     2041 acagccgggt cgttcccgaa ctgctcaccc agcgcgaacc cggcgatatg ttcgtcgtcc
     2101 gcaacgccgg caacatcgtg ccgtcctacg gccccgaacc gggcggcgtg tcggccacgg
     2161 tcgaatatgc ggtggcagcg cttggtgtgc gcgacgtggt gatttgcggc cattccgatt
     2221 gcggcgcgat gaccgcggtg gcccatgacc acgatctcca tcacatgcct gcggtgtgca
     2281 gctggctgcg ccacgccgac gccgcaaagg cggtgaacaa cgcccatgac cacggctccg
     2341 acgccgcaaa gctcgacggc atggtgcgtg agaatgtgat cgcccagatc gccaacctgc
     2401 gcacccaccc ttcggtggcg ctggcgctcg cccagggcaa gctgaccctg cacggctggg
     2461 tctacgacat cgagagcggc gcaatcgacg cgatcgacgg cagcaccggc cgcttcgttc
     2521 cgctcacgga aaatcccgac gccagtgcca ccggcgctcc ccggctttga ccgtaccatg
     2581 atcaccgtat cggtttcccg cgacgtggat cgccacggga actgacgcct cctccctcca
     2641 accaaccacc cctgaagatt gaaaggatat cattatgttg cagcaccagg tcagccagtc
     2701 ggcgcgccag aatctcaccg acgtcgtcat cgccgccaag gccgcgaagg atctttcgtg
     2761 gaagaagatc gccgagggta ccggcttcgc caaggaattc gtcaccgccg ccctgctcgg
     2821 ccagcacctg ctgccgcgtg aggcggccac gcttgtcggc gagcggctcg ggctcgatgc
     2881 cgagcaggtg cacctgctcg aagcggtgcc gctgcgtggc agcttccccg acggcgtgcc
     2941 caccgacccg acgctctatc gcttctacga gatgatccag gtctacggca cgacgctgaa
     3001 ggcgctggtc cacgaggagt tcggcgacgg catcatcagc gcgatcaatt tcaagctgaa
     3061 ggtcgagaag gtcgaggatc ccgacggcgg ccaccgcgcg gtcatcacgc tcgacggcaa
     3121 gtatctgccg accaagccgt actgaaccca atccccatcg gggctcgtgc ttcgcacggg
     3181 ccccgttttt gcacctatac tactgcacga actgccagcg acggctgctc cagcagcccc
     3241 gacagcacca gcagcgcatc ctccagcgcg gcgcgatccg gcgccacacc cagcgacacg
     3301 cgcaccgcct cgaccggcga cggcgaaatc gaaaacaccg tgctgggcac gatcgcgaca
     3361 cccagacgat cggcatgatc ggcgaaatcg gtcgcccgcc aatgctcggg caggcgcagc
     3421 cagagatggt ggccatgcgg atcg
//
LOCUS       DATGEM010000692         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1426078, whole genome shotgun sequence.
ACCESSION   DATGEM010000692 DATGEM010000000
VERSION     DATGEM010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426078"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1364
                     /locus_tag="VKQ27_21305"
     CDS             60..1364
                     /locus_tag="VKQ27_21305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91535.1"
                     /translation="MAASPISARMRRMQWIAIGLATAAIALNYVDRSTLAIGNLKIRE
                     EFGINATAIGALQSAWSITYALAQVPIGYFLDRLGPRWLVGVALILWSMAQAAGGFAL
                     SYVQLLWARVALGAAECPAFPAAVRVTSDWFHVKDRGRPTGVYNSGGSIGPFIAPPLL
                     TGLMLAFGWRAMFITMGFVGILGALVWFRLYRDPATAPLEPQDQAYLATNRAAEGKVE
                     ARNWGKLFRFQSMWGLMLGAFCTGYAVWMYQTWLPAYLEMQQHISIAKTGFVASIPLF
                     CSILGAWSGGWFTDRLSARGMDLVASRRLPSIIGLLLSGLFTVIATTAAGPTQAIVYI
                     SLAMFFLSLGIAGKWTLITAVAPQSYCTSVASIQNFGGYIGGTASPIVTGFVVDTTGS
                     FVIALAIGAGITILGAGLLQVLVRSQITSADLEGGLAAVPAQ"
     gene            complement(1387..2346)
                     /locus_tag="VKQ27_21310"
     CDS             complement(1387..2346)
                     /locus_tag="VKQ27_21310"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLY91536.1"
                     /translation="MRISLIGVSHWHTPFYLDPLLAMSDVSVIGVSDPELPRAETTAA
                     KAHCPAFTDYREMCARLKPDFVFALGRHCDMAAEARFLIEQHIPFAMEKPCAITAADA
                     HDIAARATAADVFAAVPFVIRYSPLIETIRSFGETPLYVVFKFVGGMVDRYHEQRVEW
                     MLHRSTAGGGPLINLGVHFLDLCRVLLPDTSLRVVGATMSNHFSHLDIEDHAMVLMRS
                     GNATCSVETGYLYPAPNSVFDLHYSIRTASRYFAARDNQTLEIVDNNRRREVRSMPLT
                     NSFFYPTFVQDTLRRVAAAEKPIADLTDMAAAMDLVEAAYTAA"
     gene            complement(2518..3588)
                     /locus_tag="VKQ27_21315"
     CDS             complement(2518..3588)
                     /locus_tag="VKQ27_21315"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLY91537.1"
                     /translation="MPRGETGNVADGLENVVAAETILSKVDGDAGVLILRGHYLQRIA
                     GHHSFEWATQLLWQDFVDGPVALGPARVRAFEQLRDLLPMVGALPPLAAVRLALASVP
                     DDADDAPQLLTATVAVALAAAVRRRDGRAPLAPDPKLCHAADFLRMLKGELPTQEDID
                     AFETYLLCSVDHGLNASTFTARVVASTQASLSDAVTAALCALKGPLHGGAPGPVLDML
                     DSIGPVENAEAWLDAAVMNGDRLMGFGHRMYRVRDPRCDVVKAVVARLNRNAGRLRYA
                     EAVEAAALAVLEKRKPQRSLQTNHEFYTALLLEALGLPRDAFTGAFAVARVVGWIAHA
                     LEQRADGRLIRPQSEYVGPMPA"
     gene            3668..>4707
                     /locus_tag="VKQ27_21320"
     CDS             3668..>4707
                     /locus_tag="VKQ27_21320"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HLY91538.1"
                     /translation="MSSRWFTAEQATAELGVSRATLYAYVSRGRIGVTAAPEEPRRSL
                     YDAADVKRLAERNRSGRSRRAVAASTISWGEPILTSAITRIEGGRLEYRGQDAVALSA
                     SVTLEDVAALLWQVDALPHSPSGGAWPRARGPVGVAERCIAAIADLAMAGRWAGKVET
                     VLPDAMRILDRVAWSAAGLPGSAVKPSALPLHERLASAWGASEKAADMIRRTLVLMAD
                     HELNASTYATRVVASTRAPLGACVLGGLAALVGPLHGGVTNELRAFLADPAVVADPAG
                     AVAIRLARGERVPAFGHPLYPDGDPRAAALVARLPLRGTALRVLEAMTAATGVPPNID
                     FALVVLEQSLRLP"
BASE COUNT          785 a         1458 c         1578 g          886 t
ORIGIN      
        1 ccaatctcgg cacggcgtga gccaataatt ccaaacagaa gaaacgactg gaggagacca
       61 tggccgcctc gccgatctca gcgcgcatgc gccgtatgca gtggatcgca atcggattag
      121 ccaccgcggc gattgccttg aattatgtgg accgttccac actcgccatc ggcaatctga
      181 agatccgcga ggaattcggc atcaacgcga ccgcgatcgg cgcattgcaa tcggcctggt
      241 cgatcaccta cgcattggcc caggtgccga tcggctattt cctcgatcga ctggggccac
      301 gctggctggt cggtgtcgcg ctgatcctgt ggtcaatggc gcaagcagcc ggcggctttg
      361 ccctgtccta tgtgcaattg ctctgggcac gcgtcgcgct tggcgccgcg gaatgtccgg
      421 cattccccgc cgcggtgcgc gttaccagcg actggttcca tgtgaaggat cgcggccgcc
      481 caaccggtgt atacaattcc ggtggcagca ttggtccctt catcgcgcca ccacttctga
      541 ccggactgat gctcgcgttc ggctggcgcg cgatgttcat caccatgggc ttcgtcggca
      601 tcctcggtgc gttggtgtgg ttccggctgt accgtgatcc tgcaactgcg ccgctggagc
      661 cgcaggatca ggcctatctc gcaacgaacc gggcggccga gggcaaggtg gaagcgcgca
      721 actggggcaa gctgttccgc ttccagtcga tgtgggggct gatgctgggc gcgttctgca
      781 cggggtatgc cgtgtggatg taccagacgt ggctgccggc gtatctggag atgcagcagc
      841 acatcagcat cgccaagacc ggatttgtcg cctccattcc gctgttctgc tcgatccttg
      901 gcgcgtggag tggtggctgg ttcaccgatc ggctgagcgc gcgcggcatg gacctggtgg
      961 cgagccggcg gctgccgagc attatcgggc tgctgctgtc ggggctgttc acggtgattg
     1021 ccacaacggc ggcggggccg acgcaggcga tcgtgtatat ctcactggcg atgttcttcc
     1081 tgtcgctggg gattgccggc aaatggacgc tgatcaccgc ggtggcaccg caatcctact
     1141 gcacctcggt cgccagcatt cagaattttg gcgggtatat cggcggcacc gcgtcaccga
     1201 tcgtgacggg cttcgtggtg gatacgaccg gatcgttcgt cattgcgctc gccatcggtg
     1261 cgggaattac gattctcgga gcggggctgt tacaggtgtt ggtgcggtct cagatcacct
     1321 cggccgatct ggagggcggg ctcgccgcag tgccggcgca atagcgggca acactgcgcc
     1381 cggcgcctat gcggcagtat aggccgcttc gaccaaatcc atcgctgccg ccatgtcggt
     1441 gagatcggct atcggctttt cggcagctgc gacgcgccgc agtgtgtcct ggacgaaggt
     1501 aggatagaag aaggagttgg tcagcggcat cgagcgcacc tcgcggcggc ggttgttatc
     1561 gacaatctcc agcgtctggt tgtcgcgcgc ggcaaagtag cggctcgcgg tgcggatgga
     1621 gtaatgcagg tcgaagaccg agttcggagc tggatagaga tagcccgtct cgactgagca
     1681 cgtagcattg ccgctgcgca tcaggaccat tgcgtggtcc tcgatgtcga ggtgggagaa
     1741 gtggttggac atcgtcgctc cgaccacgcg caggctggtg tctggcagca gcacacggca
     1801 caggtcgagg aagtgcacgc cgagattgat cagcggccca ccgccggccg tcgagcggtg
     1861 cagcatccat tcgacgcgct gttcgtgata gcggtcgacc atgccgccga cgaatttgaa
     1921 caccacgtag agcggcgtct ctccgaatga acgaatggtc tcgatcagcg ggctgtagcg
     1981 gatgacgaag ggtaccgcgg cgaacacgtc ggcagccgtg gcgcgggcgg cgatgtcatg
     2041 cgcgtcggcg gcagtgatcg cgcagggctt ttccattgcg aatgggatgt gctgttcgat
     2101 caggaatcgc gcttccgccg ccatgtcgca atgccggccg agcgcaaata cgaagtccgg
     2161 cttcaggcgt gcgcacatct cacgatagtc ggtgaacgcc gggcaatgcg ccttcgccgc
     2221 tgttgtctcg gctcgtggca gctccggatc gctgaccccg atcaccgata cgtcggacat
     2281 ggccagcagc ggatccaggt aaaacggggt atgccaatgt gagacgccga taagggatat
     2341 gcgcatggtc gtttccttct ggctcctcgc tcactgtgag aaggggcaca gagaagactg
     2401 tatgcgccag ccggacgctg tccatctcgg caccagcggc tgcattgcga gagaagcaat
     2461 ccgcacgaga ttgcttcgcc gctttcgcgg gggtcgcaat gacagctgga tgccgctcta
     2521 ggcaggcatc ggaccgacat actccgactg cgggcggatc agcctgccgt ctgcccgctg
     2581 ctccagcgcg tgggcgatcc atcccaccac gcgggcgacc gcgaaagcgc cggtgaatgc
     2641 gtcgcgcggc aggccgagag cctccagcaa aagcgcggtg taaaactcgt ggttggtctg
     2701 caaggatcgc tggggcttgc gcttctcgag cacggccaat gcagcagctt caaccgcctc
     2761 ggcatagcgc agtcttccgg cgttgcgatt gagtctcgcc accactgcct tcaccacatc
     2821 gcagcgggga tcacgcacgc gatacatccg atgcccgaac cccatcagcc gatcaccgtt
     2881 catgaccgcg gcatcgagcc aggcttcggc attttccacc gggccgatcg aatcgagcat
     2941 atcgagcacc ggcccaggcg cgccgccgtg cagtggcccc ttcagcgcgc acagtgcggc
     3001 agtgaccgca tcgctgaggc tcgcttgcgt cgatgccacc acgcgcgcgg tgaaagtgga
     3061 ggcattcagt ccatgatcaa ccgagcacag caggtaggtt tcgaacgcgt cgatgtcttc
     3121 ctgtgtgggc agttcgccct tcagcatgcg caggaagtcc gcggcatggc agagcttggg
     3181 atcgggagcc agcggcgctc gtccatcacg ccgacggacc gcggcagcca gtgcaacggc
     3241 cacggtcgcc gtcagtagtt gcggtgcgtc atcggcgtca tccggcacgc ttgccagtgc
     3301 cagccgcaca gcagcaagcg gaggcagtgc tccgaccatc ggcagcaggt cccgcagttg
     3361 ctcgaaggca cgcacgcgcg ccggccctag cgcaaccggc ccgtcgacaa aatcctgcca
     3421 gagcagctgg gtggcccact cgaagctgtg atgcccagcg atccgctgga ggtagtggcc
     3481 gcgcaggatc aggacgccgg cgtcaccatc gaccttgctc aggatggtct ccgccgcgac
     3541 cacattctcc agtccgtcgg caacgttgcc tgtctcgcca cgtggcatgt gcgtcctcct
     3601 tggccggttg acgccgcgat ggcatactca agatcaactt tcgtcaatct tgatcaagat
     3661 aatcaatatg tcgtctcgct ggtttaccgc tgaacaggcc acagccgaac tcggggtgtc
     3721 gcgcgcgact ctgtacgcgt atgtcagccg cggccgcatt ggtgtcaccg ccgcgccgga
     3781 agagccacgc cgcagcctgt atgacgcggc cgatgtgaag cggctggcgg agcgcaatcg
     3841 gagcgggcgc agccgccgtg cggttgctgc ctccacgata agctggggtg agccgatcct
     3901 gacctcggcc attacgcgga tcgagggtgg tcggctggaa taccggggac aggacgccgt
     3961 ggcgctgtcg gcatccgtta cgctggagga tgtggcggcg ttgctctggc aggtcgacgc
     4021 gctgcctcat tcaccatcgg gcggtgcctg gccacgcgcg cgggggcctg ttggcgtggc
     4081 cgaacgctgc atcgcggcga tcgcggatct ggcaatggct ggccgttggg caggcaaggt
     4141 tgagactgtg ctgcctgatg cgatgcgaat cctggaccgt gttgcgtggt ccgctgccgg
     4201 cttgccgggg agtgcggtga agccatcagc cttgccgctg cacgagcgcc tggcgtccgc
     4261 atggggtgcc agcgagaagg ccgccgacat gatccgtcgc actctggtgc tgatggccga
     4321 tcacgaactg aatgcctcga cctatgccac ccgtgttgtt gcctcgaccc gggccccgct
     4381 tggggcgtgc gtgctgggag gcctggcggc gctggttggg ccgctgcacg gtggcgtcac
     4441 caacgagctg cgcgcattcc ttgccgatcc ggcggttgtg gccgaccctg ccggcgcggt
     4501 cgcgatccgg ctggcgcgcg gcgagagggt gccggccttt ggccacccgc tctatccgga
     4561 tggcgaccct cgcgcggcag cattggtcgc gcgtcttcct ctgcgtggca cggcgttgcg
     4621 ggtgctcgaa gcaatgacgg cggccactgg tgtgccgccg aacatcgact tcgctctggt
     4681 ggtgctggag cagagcctgc gtctacc
//
LOCUS       DATGEM010000693         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_151449, whole genome shotgun sequence.
ACCESSION   DATGEM010000693 DATGEM010000000
VERSION     DATGEM010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151449"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..579
                     /gene="tssJ"
                     /locus_tag="VKQ27_21325"
     CDS             109..579
                     /gene="tssJ"
                     /locus_tag="VKQ27_21325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HLY91539.1"
                     /translation="MIPRRSLVLLPALIAPLALLRCAPPPKPPPVLTITMVGSKDQNP
                     DTDGTAKPVAVRIYQLAATAKFERGDVFALTEHEQQTLGQDDLGSQEFLLAPGETQTQ
                     TFGLKNGVNAIGVVVLYRDIDKSQWRADAPAADSGPTKLLLNVGKLAITLKPAT"
     gene            611..1933
                     /gene="tssK"
                     /locus_tag="VKQ27_21330"
     CDS             611..1933
                     /gene="tssK"
                     /locus_tag="VKQ27_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HLY91540.1"
                     /translation="MSWTNRVVWQEGMFLRTQHFQQQDRWTEQLVRGRVQALRPHPWG
                     LVEYAMDRDLLGTGRFALASAAGVFEDGTPFALPGEADHPPPLELPESARNVLVYLAL
                     PVRQAGAVEVADAATEGRYAAKPFEAYDTHSASPQPAELQIGRLRMRYLLETDERTGY
                     LTIPLARVTEVTADRRIILDERWIPPTLVCSGAAPLSGLIAELSGMLNQRGEALAARM
                     TAPGQAGVAQVQDFLLLQTINGWQNVLAHWADAANVHPETLYTTFVQMAGEFATFLET
                     RRPTAYPAYRHDDLQRSFAPVVADLRRALATVLESSAIPIPLREARHGVRVGPITDRS
                     ILRASNFVLTVQADVQAEQLRRLFPSQVKIGAVEHIRELVNVALPGIAVRPLPVAPRQ
                     LPFYAGATYFELDRASPHWQQMQNSGGFAIHVSGEFPNLRLELWAIRG"
     gene            2082..3449
                     /gene="icmH"
                     /locus_tag="VKQ27_21335"
     CDS             2082..3449
                     /gene="icmH"
                     /locus_tag="VKQ27_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HLY91541.1"
                     /translation="MSDNPFSEPDDSDRTVIRPAPGGRRPSASPPQQPPSQQPSAFQS
                     APAPRAPAAPMPAEGAERVVFGLNPLVEAAGPLLQLLGRLRNTYSQPDPGELRERAIQ
                     QVRAFEQAARDANVPMDQLRPAHYALCASLDDVVLNTPWGSSGAWAARSMVSSFHQEV
                     RSGERFFVLLDQLKPNPGNFLPVLELMYLCLSLGFQGQYRLSPRGPGELDRIREDLYT
                     IIMRQRQAADPGLSPHWQGIQAPYKPARAVVPTWVMAAAALAIIGILFVWFSTGLNAA
                     SDDVFAGLIGAPLSKMPAITRAAPVKPPPPPPAPPALDPLYVFLKPEIDQGLVTVLGD
                     HAMPIVRIKNTGLFASGSATVAPSYYNLLTRIGEALKAEKGPVTVDGYTDNQPIKTVQ
                     FPSNFQLSQARADAARTIIVKALGDPARVTAQGHADADPLASNATAEGREQNRRIEVV
                     LRRQG"
     gene            3453..>6926
                     /gene="tssM"
                     /locus_tag="VKQ27_21340"
     CDS             3453..>6926
                     /gene="tssM"
                     /locus_tag="VKQ27_21340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HLY91542.1"
                     /translation="MRAVLRFLGSRWFLSFIGVALLSVLVWLFGPFLSFLESWITRAI
                     VIAVMLLIWLGVNFWLDRRRKKNEDDLVQGVTAAQADPSQAASAEEVAAMREKLTTAL
                     ALLKKASGSRGYLYEQPWYAIIGPPGAGKTTALLNAGLSFPLAAEMGQSSVAGVGGTR
                     MCDWWFTENAVLIDTAGRYTTQDSDSAVDKAGWQAFLGLLKRTRARQPLNGVLIAIAL
                     SDIAAAPQAERLAHARAIRRRVKELYDQLGVRVPVYALFTKADLIAGFTEFFDDLDRE
                     RRGQVWGTTFPLDKSEAGTASKFGGEFQSLVERLNQRLLDRLQTERSPDRRSLIAGFP
                     AQVASLAAPLTEFLTEAFGGSRLDPAPVLRGAYLTSGTQEGTPIDRLTGAMARSFGID
                     AQRAPSLRPEQGRSYFLARLLKEVIFGEAMMVSRDPAQVRRNLFVRVGAAALAALVAI
                     VGAGALIQTRQANQAAVADSSNALAAYTKLAQSLPLDPVADADVPRVLPLLDAARALP
                     FGVDAPPPPMQWFPGLSQTAKLGTGAREVYRNDLEHVLLPRLIVRLEAQMRANFEQPA
                     FLYQATKVYLMLGSAGPLDRDLVKEWMHLDWQSAWPGPAAQPIRDSLARHLDALLDQP
                     LEKVSLDGTLIEDARRTFSRVTLAERVYSVIKGSGAARALPPWRPADAAGASGVRVFV
                     RKSGAPLTDGIPGFFTVDGFYKVLLPDLPAATRQVASESWVLGKQSEINPSSPQVLNL
                     QRDVITLYTDDYAKQWDAMLSDLDVAPMRNLQDAVQELYIFASPQSPMRDLLASITRQ
                     LTLTKPPPPPPGAAGAAQGAAQAATAAASQALTSTTAGLQNLFGQTNGPPPPPPGKTI
                     DDHYAALIKFVGTGPGAPIDNALKLLNDLQVQLAKLAGTPTGGTAAAIGGGDDPAALL
                     QAEAAGDPEPVSRWLKAMAFSSNQLRGGGAADQAKKAFNAPGGPASLCKQAVAGRYPF
                     TPGASNAIPLDDFSRLFAPGGLLDKYFNDNLASFVDTSAQVWKTQAVAGVAAPVSPGD
                     LAEFQRASQIRDLFFAGGGNQPTVRFDVTPIDTNAKQVTIDLDGQSVVYAHGPQRATS
                     ITWPGPNRMNSARLVFDPPPSSGASVLQATGPWALFRLFGMGTLEQNGGSDSYKLSFT
                     VGDRSASFEIRA"
BASE COUNT         1117 a         2218 c         2404 g         1187 t
ORIGIN      
        1 cagcaagacc gtgcaggcgc tcgccgatga tttctacagt gtgttcggca aggcgtttgc
       61 ccgcgcctat gaacgctcgc tacaggacgt ttccgccaag gaccgatcat gatccctcgc
      121 cgttcactgg tactgttacc cgcgctcatt gccccgctgg cgctgctgcg ctgtgcgcca
      181 ccaccgaagc cgccgccagt gctgaccatc acgatggtgg gcagcaagga ccagaacccg
      241 gacaccgacg gcaccgccaa gccggtcgcg gtgcgcatct accagcttgc cgcgacggcg
      301 aagttcgaac gcggcgacgt gttcgctctg accgagcacg agcagcagac gctgggccag
      361 gacgatctgg ggtcgcagga gttcctgctg gcacctggcg agacgcaaac gcagacgttc
      421 gggctgaaga acggcgtcaa tgcgattggc gtcgtggtgc tgtatcgcga catcgacaaa
      481 tcgcagtggc gggcggacgc gccggccgcc gacagcgggc caacaaaact tctccttaac
      541 gttggtaaac ttgctatcac gctgaagccc gcgacctgac aatcaggcgc gaacgggggg
      601 atgccgctcg atgagctgga ctaatcgcgt ggtctggcag gaaggcatgt tcctgcgcac
      661 gcagcacttc cagcagcagg atcgctggac ggagcagctt gtgcgcggac gcgtgcaggc
      721 cttgcgaccg catccgtggg ggctggtgga gtacgcgatg gaccgggacc tgctggggac
      781 cgggcgcttc gcgctggcat ccgcagcggg cgtgttcgag gatggcacgc cgttcgcgct
      841 gcccggcgag gcggaccatc caccgccgct ggaactacca gagagcgcgc gcaacgtgct
      901 ggtgtacctg gcgttgccgg tgcggcaggc aggcgcggtg gaagtggcgg atgccgcgac
      961 cgaggggcgc tacgccgcga agccgttcga ggcgtacgat acgcattcgg cgtcgccgca
     1021 gcctgcggag ctgcagatcg gccggctgcg gatgcggtat ctgttggaga ccgacgagcg
     1081 gaccgggtat ctgacgatac cgctggcgcg ggtgaccgag gtcactgctg accgccgcat
     1141 catcctggat gagcgatgga ttccgccgac gctggtgtgc tctggcgcgg cgccactgtc
     1201 ggggctgatc gccgagctgt cgggcatgct gaaccagcgc ggtgaggcgc tggcggcgcg
     1261 catgaccgcg cccgggcagg cgggcgttgc gcaggtgcag gacttcctgc tgctgcaaac
     1321 gatcaacggc tggcagaacg tgctggcgca ttgggcggat gcggcgaatg tgcatccgga
     1381 gacgctgtac accacgttcg tgcagatggc gggcgagttc gccacgtttc tggagacgcg
     1441 gcggcccacg gcgtacccgg cgtatcggca tgacgatctg cagcgcagct tcgcgccggt
     1501 ggtggcggat ctgcggcgcg cgctggcgac cgtgctggag tcgagcgcga tcccgatccc
     1561 gctgcgcgag gctcggcatg gggtgcgggt gggaccgatc accgaccgct cgatcctgcg
     1621 ggcttcgaat ttcgtgctga cagtgcaggc agatgtgcag gcggagcagt tgcgacggct
     1681 gttcccctcg caggtgaaga ttggcgcggt cgagcatatc cgcgagctgg tgaacgtggc
     1741 gctgccggga atcgccgtgc ggccgctgcc ggtggcgccg cggcaactgc cgttctacgc
     1801 gggtgccacg tatttcgagc tggatcgcgc gagtccgcac tggcagcaga tgcagaactc
     1861 gggtgggttc gcgatccacg tgtcggggga attccccaat ctgcgactgg aactttgggc
     1921 gatacgcgga tgagtgtccc gcgaacgtac tgtgtcattt cgaggagcgc agcggtggcc
     1981 ggacttgatc cggccgagca agctcgggcg gccggtgcgc gcacaactgg tccgagatcg
     2041 cttcgtcgtt gcactcctcg cgacgacaga aggacaggcg catgagcgac aatccgtttt
     2101 ctgaaccgga tgactccgat cggaccgtca tccgccccgc tcctggcggc cgccgtccgt
     2161 ccgcatcacc accacagcaa ccgccatcac agcagccttc ggcgttccag agcgcaccgg
     2221 ctccgcgggc gcctgccgcg ccgatgcctg cggaaggcgc cgagcgtgtc gtctttgggc
     2281 ttaacccgct ggtcgaggcg gcggggccgt tgctgcaatt gcttggccgc ctgcgcaata
     2341 catacagcca gccggatccg ggtgaactgc gtgaacgcgc aatccagcag gtgcgtgcct
     2401 tcgagcaggc ggcacgcgat gccaatgtgc cgatggacca gcttcgacct gcgcattacg
     2461 cgctgtgcgc cagcctcgac gacgtggtgc tgaatacgcc ctggggcagc agcggcgcgt
     2521 gggcggcgcg ctcgatggtg tcgtcgttcc atcaggaagt gcgcagcggc gagcggttct
     2581 tcgtgctgct cgaccagctc aagccgaacc cgggcaactt cctgccggtg ctggagttga
     2641 tgtacctgtg cctgagcctt gggtttcagg ggcagtatcg gctgtcgccg cgcggccccg
     2701 gcgagctcga tcgtatccgc gaagatctct acacgatcat catgcgtcag cggcaggcgg
     2761 cggatcccgg actgtcgccg cactggcagg gcatccaggc gccttacaag ccggcgcgcg
     2821 ccgtggtgcc gacatgggtg atggcggctg ccgcgcttgc catcatcggt atcctgttcg
     2881 tgtggttctc caccggcctg aatgccgcgt cagatgacgt gttcgccggg ctgatcggcg
     2941 cgccgctgtc gaagatgccg gcaatcacac gcgccgcccc ggtgaagccg ccgccaccgc
     3001 cgcccgcacc gccggcactt gatccgctct acgtgttcct gaagccagag atcgatcagg
     3061 gactggtgac cgtgctgggc gatcatgcaa tgccgatcgt tcgcatcaag aacaccggcc
     3121 tgttcgcatc gggcagcgct acggtcgcgc catcctatta caatctactc acccggatcg
     3181 gcgaggcgct gaaggcagag aaaggcccag tcaccgtgga cggctataca gataaccagc
     3241 cgatcaagac cgtgcagttc ccgtccaact tccagctttc gcaggcgcgt gccgacgcgg
     3301 cgcggacgat catcgtgaag gcgctcggcg atcctgcccg tgtcacagcg cagggccatg
     3361 cggacgccga tccgcttgcc tccaacgcca ccgcggaagg ccgcgaacag aaccggcgca
     3421 tcgaagtcgt gctgcggagg caaggctaga gcatgcgtgc cgttcttcgt ttcctcgggt
     3481 cccgctggtt cctgagcttc atcggcgtcg cgctgctgag cgtgctggtg tggctgttcg
     3541 ggccgttcct gtcattcctg gaaagctgga tcacccgcgc catcgtcatc gcggtgatgc
     3601 tgttgatctg gctgggggtg aacttctggc tcgatcgacg gcgcaagaag aacgaggacg
     3661 acctcgttca gggcgtcacc gctgcgcagg ccgatccctc gcaggccgcg tccgccgagg
     3721 aagtcgcggc gatgcgcgag aagctgacca cagcactcgc gctgctcaag aaggcgtccg
     3781 gttcgcgggg ctatctgtat gaacaaccgt ggtatgcgat catcggtccg cccggcgccg
     3841 gcaaaacgac ggcgctgctg aacgcgggac tgtcgttccc gcttgccgcc gagatgggac
     3901 agagctctgt cgccggtgtc ggcggcacgc gcatgtgcga ctggtggttc actgagaacg
     3961 cggtgctgat cgataccgcg ggacgctaca ccacgcagga ttccgactcc gcggtggaca
     4021 aggcgggctg gcaggcgttc ctcggcctgc tgaagcggac gcgggcgcgc cagccactga
     4081 atggcgtgct gatcgcgatc gcgctgtccg atatcgccgc ggcgcctcag gcggagcgac
     4141 tggcgcacgc gcgtgcgatc cggcgtcgcg tgaaggaact gtacgaccag cttggcgtgc
     4201 gcgtgccggt ctatgccctg ttcaccaagg cggacctgat cgccggcttc accgagttct
     4261 tcgatgatct cgaccgcgag cggcgcggtc aggtctgggg cacgacgttc ccgctcgaca
     4321 agtcggaagc gggcaccgcg tcgaagtttg gcggcgaatt ccagtcgctg gtggagcggc
     4381 tgaaccagcg cctgctcgac cgtctgcaga ctgagcgcag cccggaccgg cgctcgctga
     4441 ttgcaggatt tcctgcgcag gttgcgagcc ttgcggcgcc gctaaccgaa ttcctcaccg
     4501 aagcctttgg cggatcacgg cttgatccgg cgcctgtgct gcgtggtgca tatctgacat
     4561 cgggcacgca ggaaggcacg ccgatcgacc ggctgaccgg ggcgatggca cggagcttcg
     4621 gtatcgacgc gcagcgcgcg ccgtcactgc ggccggaaca aggacgcagc tacttcctgg
     4681 cgcggctgtt gaaagaggtg atcttcggcg aggccatgat ggtctcgcgc gacccggccc
     4741 aggtgcgccg caatctgttc gtccgcgtgg gggctgcggc gctcgctgca ttggtcgcca
     4801 ttgttggtgc cggcgcgctg attcaaacgc gccaggcgaa ccaggcggct gtcgccgatt
     4861 ccagcaacgc gctcgctgca tatacgaagc tggcgcagtc gctgccgctc gatccggtgg
     4921 cggacgccga tgtgccgcgc gtcctgccgc tgctggatgc ggcgcgcgca ctgccgttcg
     4981 gggtcgatgc gccaccgccg ccgatgcagt ggtttcccgg cctttcgcag actgccaagc
     5041 tcggtaccgg tgcgcgcgag gtgtatcgca acgatctgga gcatgtgctg ctgccgcggc
     5101 tgatcgtgcg cctggaagcc cagatgcgcg cgaatttcga gcagccggca ttcctgtatc
     5161 aggccaccaa ggtctacctg atgctcggct ccgctggtcc gctggatcgt gacctggtca
     5221 aagagtggat gcacctggat tggcagtccg cctggcccgg tcccgcggcg cagccgatcc
     5281 gcgacagcct ggcgcggcat ctcgacgcgt tgcttgacca gccgctcgag aaggtatcgc
     5341 tggatggcac gctgatcgag gatgcgcgtc gcaccttcag ccgcgtgacg ctcgccgagc
     5401 gggtctatag cgtcatcaag ggttccggtg ccgcacgggc gctgccgccg tggcggccgg
     5461 cggatgcagc cggcgcttcg ggtgtgcggg tgttcgtccg caagtctggc gccccgctga
     5521 ccgatggcat tcctgggttc ttcacggtcg atggcttcta caaggtactg ctgcctgacc
     5581 tgcccgcggc aaccaggcag gtggcgagcg agagctgggt gctgggcaag cagtcggaaa
     5641 tcaatccgag cagtccgcag gtgctgaacc tgcagcgcga cgtgatcacg ctctacaccg
     5701 acgactacgc caagcagtgg gatgcaatgc tgtccgatct ggacgtggcg ccgatgcgca
     5761 acctgcagga cgcggtacag gagctgtata tctttgcgtc accacaatcg ccgatgcgcg
     5821 atctgcttgc cagcatcacg cggcaattga cgctgaccaa gccgccaccg cctccgccgg
     5881 gtgcggctgg cgcggcgcag ggtgccgcac aggcggcgac cgcggcggca tcgcaggcgt
     5941 tgaccagcac gacggccggc ttgcagaatc tgttcgggca gaccaatggg cccccgcccc
     6001 cgccgccggg caagacgatc gacgatcact atgcggcact gattaagttc gtcggcaccg
     6061 gtcctggcgc gccgatcgac aatgcgctga agctgctcaa cgatctgcag gtgcagcttg
     6121 cgaagctggc cggcacaccg accggaggta ctgccgccgc gattggcggt ggcgacgatc
     6181 cggcggcgct gctgcaggcc gaggcggcgg gcgatccgga accggtgtca cgctggctca
     6241 aggcgatggc gttcagcagc aaccagttgc gcggcggtgg tgcggcggac caggcgaaga
     6301 aggcgttcaa tgcaccgggt ggcccggcgt cgttgtgcaa gcaggcggtg gcgggacgtt
     6361 acccgttcac ccccggcgcg tcgaacgcca tcccgctgga tgacttctcg cggctgttcg
     6421 ccccgggcgg actgctggac aagtacttca acgacaacct ggcatcgttc gtcgatacct
     6481 ccgcccaggt gtggaagacg caggcggtgg ccggcgtcgc ggcaccggtc tcgccgggcg
     6541 atctggcgga gttccagcgg gcgtcgcaga tccgcgacct gttcttcgcc ggcggcggca
     6601 accagccgac ggtgcgcttc gatgtcacac cgatcgacac caacgcgaag caggtgacga
     6661 tcgatctcga tgggcagtcg gttgtatatg cacatggtcc gcagcgcgcg acatcgatca
     6721 cgtggccggg cccgaaccgc atgaacagcg cgcgtctggt gttcgatcca ccgccatcga
     6781 gcggtgcgtc ggtgctgcag gcgaccgggc cgtgggcgct gttccggctg ttcggcatgg
     6841 gcacgctgga gcagaacggc ggctccgaca gctacaagct gagcttcacc gtgggcgatc
     6901 gcagcgcatc gttcgagatc cgggcc
//
LOCUS       DATGEM010000694         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_286780, whole genome shotgun sequence.
ACCESSION   DATGEM010000694 DATGEM010000000
VERSION     DATGEM010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286780"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VKQ27_21345"
     CDS             <1..314
                     /locus_tag="VKQ27_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HLY91543.1"
                     /translation="QSDVMLVCGTVTKKFAPVMRTIWDQMSEPKWCIAVGNCASAGGP
                     FPTYAVVQGIDEVVPVDIYVPGCPPIPEAMFYGIMKLQEKIKQDTIAKRVAVDVTARS
                     V"
     gene            316..804
                     /locus_tag="VKQ27_21350"
     CDS             316..804
                     /locus_tag="VKQ27_21350"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HLY91544.1"
                     /translation="MPPVPPLQHVDPESHVAVARLRATMPDAIEGVSAHRDQVTVRIA
                     AAQWVAAAQFLRDDPACSFEMLLDLTAVDYPSRPQRFDVVAHLYSLTKNHLLRIKTGV
                     AEGDSVETLVTVWKTSNWLEREAYDMFGIDFAGHPDLRRMLLPQDWVGYPLRKDYPLA
                     GY"
     gene            829..2091
                     /gene="nuoD"
                     /locus_tag="VKQ27_21355"
     CDS             829..2091
                     /gene="nuoD"
                     /locus_tag="VKQ27_21355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HLY91545.1"
                     /translation="MADNPSLEIRSLEPQAGDAAAKALLADAPHPVQEQELLLNMGPQ
                     HPSTHGVARVLLELDGEVVRKAIPDIGYLHRGIEKIGEYRQYNQFVPWTDRMDYIAAF
                     TSNLAWVQAVEGLMGIEIPPRAQFLRVLFSELQRIASHCLWLGTHVMDLGAITVFLFA
                     MRERELILDLLEMQTGARLTLNAQRVGGFPKDLPPGFLEKLEEFLKVMPGHIHEYERL
                     LAEQPIFLRRVKGVGVISAAEAVQWGLTGPTLRGSGVAYDVRKARPYAAYPHMEFDIP
                     IGTHGDVYDRYLVRMEEMRQSLRIIRQAVDLLPGGPVMTEDIRVALPGKEALMTSIEA
                     MQRHFILTIYGLRPPVGEYYSAVEAPKGELGWYIVSHGESNPYRARCRPASMINLQAL
                     PVMAEGGQIADVVACIGSIDIVMGEVDR"
BASE COUNT          363 a          693 c          683 g          354 t
ORIGIN      
        1 gccagagcga cgtgatgctg gtctgcggca ccgtgaccaa gaaattcgcc cccgtgatgc
       61 gcacgatctg ggaccagatg tccgagccga agtggtgtat cgccgtcggc aactgcgcct
      121 cggccggggg cccgttcccg acgtatgccg tcgtgcaggg gatcgacgag gtggtgccgg
      181 tcgacatcta tgtcccgggc tgcccgccga tcccggaggc gatgttctat ggcatcatga
      241 agctgcaaga gaagatcaag caggacacga tcgccaaacg ggtcgccgtg gacgtcacgg
      301 cgcggagcgt ctgagatgcc gcccgtcccc ccgcttcagc atgtcgatcc cgagagccat
      361 gtggccgtcg cccgcctgcg cgcgaccatg ccggacgcga tcgaaggcgt gagcgcccac
      421 cgcgaccagg tcaccgtgcg catcgcggcg gcccagtggg tcgcggccgc ccagttcctg
      481 cgcgatgatc cggcgtgtag cttcgagatg ctgctggacc tgaccgccgt cgattacccg
      541 tcgcggcccc agcgctttga cgtggtcgcc catctctaca gcctgaccaa aaaccacctg
      601 ttgcgtatca agaccggggt ggccgagggc gacagcgtgg agaccctggt caccgtctgg
      661 aaaacgtcga actggctgga gcgcgaagcc tatgatatgt tcggcatcga tttcgccggg
      721 cacccggacc tgcgccgcat gttgttgccg caggactggg tcggctatcc gttgcgcaaa
      781 gattatccgc tcgccgggta ttaacgggcc cagggaaagg ggactccgat ggcagacaac
      841 cccagcttag aaattcgctc gctggagccg caggccggag acgccgcggc taaagccctg
      901 ctcgccgatg cgccgcatcc cgtccaggag caagagctgc tcctgaacat ggggccgcaa
      961 caccccagca cgcatggcgt ggcgcgggtg ctgctggagc tggacggcga agtggtccgc
     1021 aaggcgatcc ccgacatcgg ctacctgcac cgcggcatcg agaagatcgg cgaataccgc
     1081 cagtataacc agttcgtgcc ctggaccgac cgcatggact atattgcggc cttcaccagc
     1141 aacctggcct gggtgcaggc cgtggaaggc ctgatgggca tcgagatccc gccgcgcgcc
     1201 cagttcctgc gcgtgctctt ttcggagctc cagcgtatcg cctcccattg cctctggctc
     1261 ggcacgcacg tcatggacct cggcgcgatc accgtcttcc tgttcgccat gcgcgagcgc
     1321 gagctgatcc tcgacctgct ggaaatgcag accggcgccc gcctgacgct caacgcgcaa
     1381 cgggtcggcg gcttccccaa ggatctcccg cccggcttcc tggagaagct ggaagaattc
     1441 ttgaaggtga tgccgggcca catccacgaa tacgagcgcc tgctggccga gcagccgatc
     1501 ttcctgcgcc gcgtcaaggg cgtcggcgtg atctcggcgg ccgaagcggt gcaatggggc
     1561 ctgaccgggc cgacgctgcg cggctccggc gtggcctacg acgtgcgcaa ggcccgtccg
     1621 tacgcggcct acccgcacat ggagttcgat atcccgatcg gcacgcatgg cgatgtctac
     1681 gaccgctacc tggtgcgcat ggaagagatg cgccagagcc tgcgtatcat ccgccaggcg
     1741 gtcgacctgc tgcccggtgg tccggtcatg accgaggaca tccgcgtggc gctgccgggc
     1801 aaagaggcgc tgatgacctc gatcgaggcg atgcagcgcc actttatcct gaccatttac
     1861 ggcttgcggc cgccggtcgg cgaatattac tccgccgtcg aggcgccgaa gggcgagttg
     1921 ggctggtaca tcgtcagcca cggcgagtcg aatccctacc gggcgcgctg tcgccccgcc
     1981 tcgatgatca acctgcaggc cttaccggtg atggccgaag gcggccagat cgccgatgtc
     2041 gtcgcctgta tcggcagcat cgacatcgtc atgggtgagg tggaccgcta atg
//
LOCUS       DATGEM010000695         6266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_741467, whole genome shotgun sequence.
ACCESSION   DATGEM010000695 DATGEM010000000
VERSION     DATGEM010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741467"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VKQ27_21360"
     CDS             <1..875
                     /locus_tag="VKQ27_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY91546.1"
                     /translation="YVTWMRDERGFTPSTVELWSRTTKRFLRWCAEAGRQLGDLTAAD
                     IDNYVATQGKGRWARVSTANMVSALRAFLRYAAQEGWCSNRLVESIGRPRLYQQESLP
                     YAPDWSAVQQMLADVDTDRPRDIRDRAILLLLALYGMRSGEVTALRLEQIDWAGRTLG
                     LVRLKRRQPQIYPLLPAVAEALALYIDTVRPRSSHPEVFLCMHAPLRPLKAGSIFDVA
                     NRRFVALGIEAAHRGGHALRHACAVRLLTEGLTLKEIGDHLGHRSTSATSIYAKVDMA
                     ALREVGAFDLGGVL"
     gene            875..1816
                     /locus_tag="VKQ27_21365"
     CDS             875..1816
                     /locus_tag="VKQ27_21365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY91547.1"
                     /translation="MLADVIDAYLARQRSLGARFDSAERLLRRFSRAMGDRPIQEVTP
                     GAIRDFLNGNGILSTTWALKYRVLTGLYRFAVSRGYVDESPLPTDLPKLPPQQTPYVY
                     STDELRRLLEATAILSAGRSRQVATMYRTLLLLLYGTGMRIGEALRLTLQDVDLAERV
                     ITVRCTKFFKTRLVPIGPKLADELVAHLERRGLLPMPLGKAAPLFASRGVRGWSYPRV
                     ITMFQHVRRAAGINCPVGEPRPPRLHDIRHTAAVHRVVAWYRAGQDVQRLLPQLATYL
                     GHLDIRSTQRYLQMTPELLQEASQRFAQYAMGNDHEG"
     gene            1806..2801
                     /locus_tag="VKQ27_21370"
     CDS             1806..2801
                     /locus_tag="VKQ27_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLY91548.1"
                     /translation="MKDETLLGPWVRRFLIEHLVAERNLARNTQVSYRDTLALLLPFA
                     SKHGGHAIDRMAVEDLTPSIIRKFLDHLERDRQCSGVTRNQRLATIRSLARFIGMRSP
                     VHVAWCAEIRTMPFKKTARTVIGYLEKGEMDALLAQPDQRTPLGARDHALILFLYNSG
                     ARADEAARLTIDNLQLGTQSHVQLHGKGNKLRTCPLWPSTAMSLSRLVANRGKSDAVF
                     LNRSDQPLTRFGIHRLVTQYAERASRTVPSIATKRVSPHTIRHTTAVHLLRAGVDINT
                     IRAWLGHVSLDTTHVYAEVDMEMKAKALARVDISDLETKPRHRQLPSLMMFLKAL"
     gene            2923..4122
                     /gene="istA"
                     /locus_tag="VKQ27_21375"
                     /pseudo
     CDS             2923..4122
                     /gene="istA"
                     /locus_tag="VKQ27_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            4134..4874
                     /gene="istB"
                     /locus_tag="VKQ27_21380"
     CDS             4134..4874
                     /gene="istB"
                     /locus_tag="VKQ27_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HLY91549.1"
                     /translation="MLTHPTLDQLHALGLQGMAKAFTELDRHGDASTLSHAEWLALLL
                     DREATWRHDKRLSARLRHAKLRHQAAPEDVDYRSPRGLERRLFEVLLKGEWIDAHDNV
                     AICGPTGIGKSWLACAIGHKACRDNRSVLYVRFTRLLDELALARGDGRIASRLKSLAS
                     VELLILDEFGLQALDAQARHDLLEILEDRYGRKSTIVTSQLPTASWHHAIGDSTYADA
                     ILDRLLHNAHRVELEGDSLRRTKASISD"
     gene            5001..5381
                     /locus_tag="VKQ27_21385"
     CDS             5001..5381
                     /locus_tag="VKQ27_21385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91550.1"
                     /translation="MREHSKGGFVFPGDKPEKPISQNTMIYGCYRMGYRGRQTVHGFR
                     GIASTWANEAECYRPDWIEMALAHAERDEVRGAYNSALYLTPRRRMLQNWGDAIADMM
                     SPARAQQAADVDEDSGCSIVPELT"
     gene            5569..>6266
                     /locus_tag="VKQ27_21390"
     CDS             5569..>6266
                     /locus_tag="VKQ27_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLY91551.1"
                     /translation="MEQSSVCVVDANGKIVREAKVLSEPDALIAWFDALGLAMERIGL
                     EAGPLSQWLYAAMARAGLAVELLETRHVRRAFEIMPVKTDKKDVRGIAQLMRLGWFRP
                     VHCKSLPAQEIRAVLTARKILQSKLHDIDLCLRGILRGFGLKVGKTTPRTFAARIREL
                     AAGHPTLEVIASSLLKAREALASEFVGFERRVRAMARENADARRLMSTPGVGVLVAMT
                     FVAAVDAPERFRSSR"
BASE COUNT         1153 a         2002 c         1950 g         1161 t
ORIGIN      
        1 ggtatgtcac ctggatgcgc gatgagcgcg gcttcacgcc atcgacggta gagctatgga
       61 gccgtacgac caagagattc ctgcggtggt gcgcggaggc gggtcgccag ctcggtgatt
      121 tgacggcggc cgatatcgat aactacgtcg ccacacaggg caagggccgc tgggcacgcg
      181 tctcgacagc caacatggtc tcggcattgc gcgcgttctt gcgctatgct gcccaggagg
      241 gatggtgttc gaaccgattg gtagagtcga ttggccggcc tcggctctat cagcaggaat
      301 ccctgccata tgccccggat tggtctgccg ttcagcaaat gctggccgac gtcgacaccg
      361 acaggccacg ggatattcgt gatcgcgcca tccttctgct gctcgcgctc tatggcatgc
      421 ggagcggtga agttacggcc ctgcggctcg aacagattga ttgggcgggg cgaaccctcg
      481 ggctcgttcg gctgaagcgg cgacaacccc agatctatcc tctgctacct gctgtcgctg
      541 aggcgctagc tctttacatc gacaccgtcc gcccgcggtc atcccacccg gaagtgttcc
      601 tgtgcatgca cgcgcctctt cggcctttga aggcgggcag catctttgac gtagctaatc
      661 ggcggttcgt cgcgctcggt atcgaagctg cgcatcgcgg agggcatgcg ttgcgtcacg
      721 cctgcgcggt tcgtctgctc actgaaggcc tgaccctcaa ggaaatcggc gatcacctgg
      781 gacaccgcag cacgtcggca accagcatct acgcgaaggt ggacatggct gctcttcgtg
      841 aagtcggcgc cttcgatctg gggggcgtcc tatgatgctg gccgacgtca tcgatgccta
      901 tctggcacgc caacggtcac tgggcgcacg gttcgattcc gccgaacggt tgctccgtcg
      961 cttcagccgg gcaatgggcg atcgaccgat ccaggaggtg acacccggag ctatccgcga
     1021 cttcctcaac gggaatggga ttctgtccac aacatgggcg ctcaaataca gggtgctgac
     1081 cggtctgtac cggttcgcgg tcagtcgcgg atacgtcgac gaatcgcctc tgccaacaga
     1141 tctaccaaag ctgccgccac agcaaactcc ttacgtttat tcgaccgacg aactgcgtcg
     1201 actgttggaa gcgacagcga tcctctccgc tggtcgcagc cgtcaagtcg caacgatgta
     1261 ccgtacgctt ctgttgcttc tctacggaac cggcatgcgt attggggagg cattgcgctt
     1321 gaccttgcag gatgtcgacc tcgccgaaag ggtcatcacc gtccgctgca caaagttctt
     1381 caagacgcgt ctggttccga ttggacccaa gcttgccgac gaactggtcg cgcacctcga
     1441 acgccgcggc ttgttgccca tgccactcgg gaaagcggcg ccgctgtttg cctcgcgggg
     1501 cgtccgagga tggagttatc cccgcgttat cacgatgttc cagcacgtgc gacgagccgc
     1561 tggtatcaac tgcccggtcg gcgagccgcg cccaccgcgc ttgcatgata ttcgccacac
     1621 tgcagccgtg catcgcgtcg tcgcatggta tcgcgccggc caggatgtcc agcgattgct
     1681 tccgcagctc gctacctacc ttggtcacct cgatatccga tcgacccagc gttacctcca
     1741 aatgacgccc gagctcttgc aggaggccag tcaacgcttc gcccagtacg cgatggggaa
     1801 cgatcatgaa ggatgagacg ctcctggggc catgggttcg gcggtttctc atcgagcacc
     1861 tggtggcaga acgcaatctg gctcggaata cccaggtcag ctaccgcgat acactggcgc
     1921 tcttgctgcc gtttgccagc aaacatgggg ggcatgccat cgaccggatg gccgtcgagg
     1981 atctgacgcc ttccataatc cgcaaattcc tcgatcactt ggaacgcgat cgccaatgca
     2041 gtggggtcac ccgtaatcaa aggctcgcga ccatccgctc cttggcgcgg tttatcggca
     2101 tgcgctcacc ggtccatgtg gcctggtgcg ccgaaatacg gacgatgccc ttcaagaaga
     2161 ctgctagaac cgtgattggg tatctcgaaa agggcgagat ggacgccctc ctggcccaac
     2221 cggatcagcg tacgcctctt ggcgcccgcg accatgcgct gatcctgttc ttgtacaaca
     2281 gcggtgctcg ggccgacgaa gcggcgaggc tgaccatcga caacctgcag ctgggaacac
     2341 aatcgcatgt gcagctgcat ggcaagggaa acaagcttcg gacctgcccg ctatggccct
     2401 ccaccgcgat gtcgctgtcg cggctggtcg ccaatcgggg caagagcgat gctgtcttcc
     2461 tcaaccggag cgaccagcct ttgacccggt tcggaatcca tcgactcgtg acgcaatatg
     2521 ccgaacgcgc cagcaggact gtgccgtcga tagcgacgaa acgcgtaagc ccgcacacca
     2581 tccgacacac aaccgcagtc catctgctgc gcgccggtgt cgacatcaac acgatacgcg
     2641 cctggcttgg tcacgtgtct ctggatacca cacatgtcta cgccgaggtc gacatggaaa
     2701 tgaaggcgaa ggcgctggcc cgggtcgata tatctgactt ggaaaccaag ccacggcacc
     2761 ggcagctacc gtcgctgatg atgttcctaa aagccctata gtcgctcgct tggcggctag
     2821 agtttatgtt gcggcccgtc cctgtgaaat cagcctttcc ttggcttgcg ccacataatc
     2881 cgggccgcaa cataacggct ggtatgtgca gcataacata tcagccggtt ttgtgagctg
     2941 tatcgcgcct gggaggggcg gcttccggtg acgatgcggc agacgcatct gggcggcgac
     3001 cggatgttcg tcgactatgc tggcgacacg gtgccggtgg tggtcgaccg gctgacgggc
     3061 gagatccgcc aggcccatct tttcgtggcg gtgatgggcg cctcgagcct gtcttttgcg
     3121 tggccgacgt ggaccgagac gctgccggac tggaccgacg cgcatgtccg cgccttcggc
     3181 ttttttggcg gctcggccag actcctcatc cccgacaatg ccaaggtcgc ggtcatcaag
     3241 gcctgccttt acgacccgca ggtcaaccgg acctattccg agctcgccgc gcattatggc
     3301 acggcaatcc tcgcagcacg gccgtatcgc ccgcgcgata aagcgaaagt ggaaaattgc
     3361 gtcgggactg tcgagcgttg gctgctcggg cggcttcgcc accggatctg gtacggcatg
     3421 gacgaactgc gcgccgccgt cgccgagatg atggccacgc tgaacgaccg gatcatgcgc
     3481 cggttcggcc ggtcgcggcg cgcgctgttt gaagagatcg accagccctt gctgaaggcg
     3541 ttaccggcag aaccttacgt cctggcggag tggcgccgct gcaaggtcgg catcgactat
     3601 catgtcgagg ccgcgaagca tttctactcg gtgccgtatc gccatgcccg agcacaggtc
     3661 gaggtgcgcc tgacggcgcg caccgtcgag attttcctca agggcgagcg gatcgcctcg
     3721 cacatgcgtg gatccggcaa tggccatcac accacgatca acgaccatat gccgtcgagc
     3781 catcgccgct acggcgattg gacgcttgag cgtcttctcg aagaggccgg gcggcttggc
     3841 ccatcagtca ggatgctgtg cgagatgatc ctgcaggacc ggccccatcc cgagcatggg
     3901 tttcgggcct gtcgcggcat cgtcgggctg gcgcgcgcct atggcgcgag ccgtgtcgac
     3961 gccgcttcgc tgcgcgcgct cgagatcggc gcgcgcaaat atggtgccgt caaatcgatc
     4021 ctcgagaaga agctcgatgg cgagccgctg cgtcgtccgc gcaccgccgg cgaggtcggc
     4081 atcgaccacc ccaacatccg cgggcccaaa tattaccact gaggagatcg ctcttgctga
     4141 cccatccgac gctcgaccag cttcacgccc ttggcctgca aggcatggcc aaggccttca
     4201 ccgaactcga caggcatggc gacgccagca cgctcagcca tgctgaatgg ctggccctgc
     4261 tgctcgaccg ggaagccacc tggcgacacg acaagaggct gagcgcgcgc ctgcggcacg
     4321 ccaaattgcg ccatcaggcg gcacccgagg atgtcgacta ccgaagcccc cgaggtctcg
     4381 agcgccggct gttcgaagtg ctgctcaagg gcgagtggat cgacgcccac gacaacgtcg
     4441 ccatctgcgg ccccaccggc atcgggaaaa gttggcttgc ctgcgccatt ggccacaagg
     4501 cgtgtcgcga caaccgctcg gtcctctacg tccgcttcac ccggctgctc gacgaactcg
     4561 ccctcgcgcg gggtgacggc cgtatcgcca gcaggctcaa gagcctcgcc agcgtagagc
     4621 tcctcattct tgatgagttt gggctgcaag ccctggacgc ccaggccagg cacgacctgc
     4681 tcgagatcct ggaagaccga tacggcagga agtcgaccat cgtgacaagt cagctcccca
     4741 cggcctcatg gcatcacgcc ataggagatt ccacctatgc ggacgcgatc ctcgaccgcc
     4801 tccttcacaa cgctcaccgc gtcgaactcg aaggcgacag cctgcggcgc accaaggcct
     4861 ccatcagcga ttgaccgcac ccttcgaaac tgacacccaa tccctgtcga ccgggtgacc
     4921 gccactgatc gcgatcatct cggaacgccg cgcgccatca tgtcggaatg gctgcgcgcg
     4981 atcattggaa tccgcactcc ttgcgcgaac acagcaaggg cgggttcgtt ttccccggcg
     5041 acaagccgga aaagccgatc tcgcagaaca ccatgatcta tggctgctac cgcatgggct
     5101 atcggggacg gcagaccgtc catggctttc ggggcatcgc ctccacctgg gccaacgaag
     5161 cggaatgcta tcggccggac tggatcgaga tggcgctcgc ccacgccgag cgcgacgagg
     5221 tgcgcggcgc ttacaacagc gccctctatc tcacgccacg tcgacgcatg ctgcagaatt
     5281 ggggcgacgc tatcgcggac atgatgagcc ctgcgcgagc acagcaagcc gcggatgtcg
     5341 atgaggatag cggctgctct attgtcccgg aattgacgtg agctagcggg gagcggccca
     5401 gtcggttctg cgcggccttc tagatggggt aacgggcgga ccctagccgg ccaggtttga
     5461 agggctggtg gtcgtccctc acaatgatgg tgttgcggag tcaggatagg cctgctccaa
     5521 gcatccggag agacgaccgt gaaccactat gccggcatcg atgtctcatt ggaacagtcc
     5581 agcgtctgcg tggtcgacgc caatggcaag atcgtacgcg aagccaaggt gctgagcgaa
     5641 ccggacgcgc tgatcgcctg gttcgacgcg ctcggactgg cgatggagcg gatcgggctg
     5701 gaagcaggcc ccttgtcgca atggctctac gccgcgatgg caagggctgg actggcggtc
     5761 gagctgctcg agacccggca tgtgcgtagg gcgtttgaga tcatgccggt caagaccgat
     5821 aagaaggacg tgcgcgggat tgcccagctg atgcggctcg gctggttccg gccggtccat
     5881 tgcaagtcgc tgcccgccca ggagatccgc gcggtgctca ccgcgcgcaa gatcctgcag
     5941 agcaagctcc acgacatcga tctttgcctg cgcggtatct tgcgcggctt tgggctcaag
     6001 gtcggcaaga ccaccccgcg aacctttgcg gcgcggatcc gcgagctggc cgcaggccat
     6061 ccgaccctgg aggtgatcgc ctcctcgctg ctgaaggcgc gggaggcact ggcgagcgag
     6121 tttgtcggct tcgagcgccg cgtgcgcgcg atggcgcggg agaacgccga cgcccgccgg
     6181 ctgatgtcga cgcccggcgt cggcgttctg gtggcgatga ccttcgtggc ggcggtcgat
     6241 gcgccggaac ggttccgatc gtcacg
//
LOCUS       DATGEM010000696         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_780187, whole genome shotgun sequence.
ACCESSION   DATGEM010000696 DATGEM010000000
VERSION     DATGEM010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780187"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..928)
                     /locus_tag="VKQ27_21395"
     CDS             complement(161..928)
                     /locus_tag="VKQ27_21395"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLY91552.1"
                     /translation="MRPNRIKQMWRDGKYVTLGWISVSHGFTAEVMARQGFDALCVDL
                     QHGTSEMKDVGPLLAAISQTDTVPVVRVAWNEPAAIMKALDLGAYAVIVPLVNNAEEA
                     AKAVAACRYPPVGMRSSGPVRAVHYGGADYQAKANDEIVIMAMIETKEGIANLEAICA
                     TPGLDAVYIGPADLSFALGLPPRGDNPDPQHLATCDKILATAHKHGIKCVMHCASAAF
                     AAGAVKRGFDMVMLTSDLSCMIAGARKELDELKAKAA"
     gene            1064..2083
                     /locus_tag="VKQ27_21400"
     CDS             1064..2083
                     /locus_tag="VKQ27_21400"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLY91553.1"
                     /translation="MSHYEQFRDSTGHLNDAATLRTRLAEDGYLFLRGLLPRQAILDV
                     RTRLLDKAAAGGWLAPGSPASAGVANQAAACKDPEPRYMSVFRTLWSDEALHRLRTHP
                     SALALFDRIFGERPLPHPMFVQRNIFPQSQDFDFTTGIHQDRVHIGGATSYAMWMPLG
                     DCPRDMGSLAVAAGSHTTGILPTKVGSGAGGMDIAVPIPGTWVTSDFAAGDALIFRDV
                     TVHKALPNRTHSIRMSFDARYQKLSEPIADVNLVPYAGCGSWDEVYAGWSSREGQYYW
                     KDLPIAVVPLDRSHYERRDAMAFEMAEAGDRDARDAILRIIQRDPLPAKRERAEKLLA
                     RLDAL"
     gene            2190..>2709
                     /locus_tag="VKQ27_21405"
     CDS             2190..>2709
                     /locus_tag="VKQ27_21405"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLY91554.1"
                     /translation="MTRLSRRNALAAAAASGAISALRLSSARAADTPQPALPVPSELR
                     ANAQGEISFSALAGTAKFLPGHATRTYGYSAPFLGPALRLRRGGKVAIDFTSHLPEPT
                     TVHWHGLIIPGYLDGGPHQPVAPGGQWRPTLQIDQPAATLWFHPHFYPTTTKQVIKGL
                     AGLLVIDDEETDR"
BASE COUNT          461 a         1002 c          773 g          473 t
ORIGIN      
        1 atcgcagcca ttctgaccgc gccgccaccg tgggacgacc gcgcggccga acggcagatc
       61 gcatggatgc tgccggtcgg cgcagtctgg tggcgttgct tcgcgtcgct ggcggatggc
      121 ggcgaagcgg agagcgcctg accctccgcc tccgcagccc tcaggccgcc ttcgccttca
      181 gctcgtcgag ctccttccgc gcacccgcga tcatgcacga cagatcggac gtcagcatca
      241 ccatatcgaa gccgcgcttc accgctcccg ccgcgaacgc agcgctcgcg cagtgcatga
      301 cgcacttgat cccatgcttg tgcgccgtcg ccaggatctt gtcgcaggtc gccagatgct
      361 gcgggtccgg gttgtcgccg cgcggcggca gccccagcgc gaacgacaga tcggcgggtc
      421 cgatatacac cgcatcaagc ccgggcgtcg cgcagatcgc ctccagattg gcgatgcctt
      481 ccttcgtctc gatcatcgcc atgatgacga tctcatcgtt ggccttggcc tggtagtccg
      541 cgccgccgta gtgcaccgcg cgcaccggcc cagatgagcg catgcccacc ggcggatacc
      601 ggcacgccgc caccgccttc gccgcttcct ccgcgttgtt caccagcggc acgatgaccg
      661 cgtaggcacc caggtccagc gccttcatga tcgccgccgg ctcattccac gccacccgta
      721 caaccggcac cgtatcggtc tgcgagatcg cagccagcag cggccccaca tccttcatct
      781 ccgatgtgcc gtgctgcaga tcgacgcaca gcgcatcgaa cccctggcgc gccatgactt
      841 cggcagtgaa cccgtgggac acggagatcc agcccaacgt cacatatttg ccgtcgcgcc
      901 acatctgctt gattctattc ggccgcatcc tcgcacctct ggcaatggtt cattgaccct
      961 ccggctatac aggcaagcgc cccgaaaccc aacagccgct catctgtctt gccagccccc
     1021 cggcaacccg cacacgatac cgcgacagcg caggaggaaa gccttgtccc actatgaaca
     1081 gttccgggat tccaccggtc acctgaacga tgccgcaacc ctgcgcactc gcctggccga
     1141 ggatggctac ctcttcctgc gcggccttct tccacgacag gcaatcctcg acgtccgcac
     1201 ccgcctcctg gacaaagcag cggcaggtgg ctggctcgcg ccaggttccc ccgcgtccgc
     1261 cggcgtcgcc aatcaggccg ccgcctgcaa ggaccccgag ccgcgctaca tgtcggtctt
     1321 ccgtaccctc tggtcggacg aggcgctgca tcgcctgcgc acccatccgt cggcgcttgc
     1381 cctgttcgat cgcatcttcg gtgagcgccc tctgccgcac ccgatgtttg tccaacgcaa
     1441 catcttccca cagtcgcagg atttcgactt caccaccggc attcaccagg accgcgtcca
     1501 catcggcggt gcgaccagct acgcgatgtg gatgccgctg ggcgactgtc cgcgcgatat
     1561 gggttccctc gctgtcgccg ctggctccca cacaactggt atcctcccca ccaaggttgg
     1621 ctccggcgcg ggaggcatgg atatcgccgt gccaatcccg ggtacctggg tgaccagcga
     1681 tttcgcggcg ggcgatgcct tgatcttccg cgacgtgact gttcacaagg cgctgcccaa
     1741 ccgcacccat tcgatccgga tgtcgttcga tgcgcgctac cagaagctca gcgagccgat
     1801 cgcggatgtg aacctcgtgc cctacgccgg ctgcggctcc tgggatgagg tctacgccgg
     1861 ctggtcgagc cgcgaaggcc agtactactg gaaggacctg ccgatagcgg tcgtgccgct
     1921 cgatcgctcg cattacgaac gtcgtgacgc gatggccttc gagatggccg aagccggcga
     1981 tcgcgacgca agggacgcaa tcctgcgtat cattcagcgt gatccgctgc cggcgaagcg
     2041 ggagcgcgct gagaagctcc tcgctcggtt ggacgcactc tgaacatccg cgcctcgcgc
     2101 aatcatcctt tcccgggcaa ctgggcaata ttcatgggca accgccgcga gagtgctgcg
     2161 tctgcacatc gcgctcggtg gaggcagcaa tgaccaggct ttcccgtcgc aacgcacttg
     2221 ccgctgccgc cgccagcggg gcaatttctg cattacgcct gagcagcgcg cgcgccgccg
     2281 acacgcctca gcccgctctg cctgttccct cggaactcag agcgaacgca cagggcgaaa
     2341 tcagcttcag tgcgcttgcc ggcaccgcga aattcctccc gggccatgcc acccgaacct
     2401 acggctacag cgcaccgttc ctaggccccg cccttcgcct gcggaggggc ggaaaggtcg
     2461 cgatcgattt caccagccat ctgccagagc cgacgaccgt gcactggcac gggctgatca
     2521 ttcccggcta tctcgatggt ggtcctcacc agcccgtcgc acccggcgga caatggcggc
     2581 ccactttgca gatcgaccag cccgccgcca cgctctggtt ccatccgcat ttttatccaa
     2641 ccacaacaaa acaggtcatc aagggcctcg ctggcctgct cgtcatcgac gacgaggaga
     2701 ccgaccgcc
//
LOCUS       DATGEM010000697         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1427466, whole genome shotgun sequence.
ACCESSION   DATGEM010000697 DATGEM010000000
VERSION     DATGEM010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427466"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKQ27_21410"
     CDS             complement(<1..412)
                     /locus_tag="VKQ27_21410"
                     /inference="COORDINATES: protein motif:HMM:NF014821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HLY91555.1"
                     /translation="MSDRNARMAARFAASHYCNMKETHMIDRRSDDQLWDQVIRREPG
                     DFFYAVTTMGIYCRPGCPSPRPLRKNVRYFRTIGEAEQAGFRACKRCDPKGDRARLAQ
                     AVVQDACAFIEASDEIPSLETLAVRSGYSRFHFLR"
     gene            complement(426..1667)
                     /locus_tag="VKQ27_21415"
     CDS             complement(426..1667)
                     /locus_tag="VKQ27_21415"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLY91556.1"
                     /translation="MKFVAVTYGTEGDTRPMALLCRALMDAGHSTHLLADAATLGYAA
                     GLGVPATALAGDIRTVLRQDTTVVAPGGGFRSTASAFARMATTNSEAWLRVTVAAADG
                     CDAVMLGGLAGFVGLSAAEALGIPAIGTGLIPITPTREFASPFLRPGMVPHRLNRVSH
                     RLVNAAIWRVFQASVNAARAGVCGLPPRRRIWTEHPMLYGVSPSLVQQPTDWPPGARI
                     CGHWLARATEWSPPPALGEFLAAGEPPIYLGFGSMGALGGASLMREALRALRGRRALF
                     YPGWSGVAADDLPANVHLLAEAPHGWLFPRVALAIHHGGAGTAHAAIRAGVPSVVAPF
                     AGDQFFWADRLRRAGVAPGLVPAKGLSAEKLACAIDAAETDEPRASASALAVSVAGED
                     GLRTAVTAIEKLMGRPAQGRP"
     gene            complement(1664..2416)
                     /locus_tag="VKQ27_21420"
     CDS             complement(1664..2416)
                     /locus_tag="VKQ27_21420"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HLY91557.1"
                     /translation="MFRTKVDATYSPLCKLKPVAPDVWVVDGPIIRFGPPLLKMPFPT
                     RMTVLRLPGERLLIHSPTPLPESLAGGVAELGRPRWIVGPNRLHYWWIPDWHAAFPDA
                     DVYLAPRIAEQARGRIDFPWLPLDPQCGLPWDADVATLAIEGSYMTEVVFFHRATKTL
                     ILTDLIENFERQRVGFCMRCLASIGHVLDPDGQTPSDMRMTFAKPALRLAVETMTRWQ
                     PERVILAHGRWYERDGTKELRRAFRWLRPVAG"
     gene            complement(2561..3409)
                     /locus_tag="VKQ27_21425"
     CDS             complement(2561..3409)
                     /locus_tag="VKQ27_21425"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91558.1"
                     /translation="MGDEPVMLQHVEGAATAGAKLRIDNVALRFTPKTGTAVTALENI
                     SLDVAEQEFSVIVGPSGCGKTSLLRLVAGLIEPTEGAIWLDAKRVTGPGRDRGMVFQS
                     YTLFPWLTVQQNVEFGLKLRGVAAVERQQIARKYLAQVGLDGFEQHYPRQLSGGMMQR
                     VALARALANDPAVLLMDEPFGALDSQTRSLMQELLLNIWQQSNKTVLFITHDVDESIL
                     LGDRVYVMTARPGRIKEVVKVELPRPRHVDLLTSEAFMTLKRRIMHSIHEEAVLSFAA
                     DEARRG"
BASE COUNT          686 a         1250 c         1034 g          565 t
ORIGIN      
        1 tgcgcaggaa atggaagcgg gaatatcccg accgcaccgc cagcgtttcc agcgacggaa
       61 tctcgtccga ggcttcgatg aacgcgcagg catcctgcac cactgcctgt gccagacgcg
      121 ccctgtcacc cttgggatca cagcgcttgc aggcacggaa cccggcctgc tccgcctcgc
      181 cgatcgtgcg aaagtaacgc acgttcttcc gcaacgggcg cggcgagggg cagccgggac
      241 ggcaatagat gcccatagtg gtcacggcat agaagaagtc ccccggctcc cggcgaatga
      301 cctggtccca gagttgatcg tccgaccgac gatcgatcat atgtgtctct ttcatgttgc
      361 aataatggga agcggcgaag cgagccgcca tccgcgcgtt gcggtcactc aatcatggcc
      421 cctcgtcatg gccgcccctg agccggccgc cccatcagct tttcgatggc cgtgacagcc
      481 gtcctcagcc cgtcctcgcc cgcgacggac accgcaagcg cggaggcact ggcgcgcggc
      541 tcatccgtct cggccgcatc gatggcgcac gcaagtttct cagcactcag gcctttggca
      601 ggcaccagcc ccggcgcaac gcccgctctc cggaggcgat cggcccagaa gaactggtcg
      661 cccgcgaacg gggccaccac cgagggcacc ccagccctga tggcagcatg agccgtgccg
      721 gcgccgccat gatggatcgc cagtgcgacc ctgggaaaca gccagccgtg cggcgcctcc
      781 gccaacagat ggacgttggc gggcaggtcg tccgccgcca caccgctcca gccaggatag
      841 aacagcgcgc ggcggccacg cagcgctctc agtgcctcgc gcatcagcga cgccccgccc
      901 aacgccccca tgctgccaaa acccagatag atcggcggct cgccggcagc gaggaactcg
      961 cccagcgcgg gcggtggcga ccactccgtc gcccgtgcca gccagtggcc gcagatccgc
     1021 gcgccaggcg gccagtcagt tggctgctgc accaggctcg gcgaaacgcc ataaagcatc
     1081 ggatgctcgg tccagatcct gcggcgtggc ggcagtccgc atacgccagc gcgggcggcg
     1141 ttgaccgagg cttggaacac ccgccagatc gccgcattga ccagccggtg gctcacccgg
     1201 ttcagccggt gtggcaccat ccccggccgc aggaatggcg aggcgaattc gcgcgtcggc
     1261 gtgatcggaa taaggccggt gccaatggcc gggatcccta aagcctctgc ggcagacagg
     1321 cccacgaagc cggccaggcc accaagcatc accgcatcac agccatccgc ggcggcaacg
     1381 gtcacgcgaa gccacgcttc ggaatttgtt gtcgccatac gggcaaaggc gctcgctgtg
     1441 cttctgaagc caccacccgg cgcgaccacg gtcgtgtcct gtcgcaacac ggtgcggatg
     1501 tcgccggcga gcgctgtcgc cggcacaccg agacctgccg cataaccaag cgtcgcggcg
     1561 tcagccagca gatgggtgct gtggccagcg tccatgagcg cccggcaaag cagcgccatc
     1621 ggccgcgtat caccttcagt gccgtaggtg acggcaacga acttcatcca gcgaccggcc
     1681 tcagccagcg aaacgcccgg cgcaactcct tcgtgccgtc tcgctcgtac catcggccgt
     1741 gcgccagaat gacccgttcc ggctgccagc gcgtcatcgt ctcgaccgcc aaacgaagag
     1801 ccggcttggc aaaggtcatg cgcatgtcgc tgggcgtttg cccatctgga tcgagtacat
     1861 gaccgatact ggccaggcag cgcatacaaa atccaacccg ctgccgctca aaattctcga
     1921 tcagatctgt gagtatcaag gtcttcgttg cccgatggaa gaacacaacc tccgtcatgt
     1981 agctgccctc gatcgcgagc gtcgcgacgt cggcatccca cggcagacca cactgcggat
     2041 ccaatggcag ccaaggaaaa tcgatccgcc cccgggcctg ttcggctatg cgcggcgcga
     2101 gatacacgtc ggcatcggga aacgcggcat gccagtctgg tatccaccag taatgcagcc
     2161 ggttcgggcc aacaatccag cgcggcctcc cgagttccgc cacgccgcca gccagactct
     2221 ccggcagcgg cgtcggcgag tgaatgagca gtcgctcgcc cggaagtcgg agcaccgtca
     2281 ttcgtgtcgg gaacggcatc ttgagcaaag gtggcccaaa ccggatgatc ggcccatcga
     2341 ccacccagac atcgggagca acgggcttca gtttgcaaag cggcgagtag gttgcgtcaa
     2401 ccttcgtgcg aaacatatca cggcaatcat ctgcacggcc tgctgatcgc taactcacag
     2461 cggcaatgct cggttgccga cgacggtcgg atttctcccg ctgcacagac gaggcacgct
     2521 catcgattgt gacatggcgc agccgcgccg caacgtgccg tcatcccctc cgtgcttcat
     2581 ccgcggcgaa gctgagcacc gcctcctcgt ggatcgaatg catgatccgc cgcttcagcg
     2641 tcatgaatgc ctcgctggtc agcaggtcca cgtgacgcgg acgcggcagc tcgaccttca
     2701 ccacctcctt gatccgtccc ggccgcgccg tcatcacata gacccggtca cccagcagga
     2761 tcgactcatc cacatcgtgg gtgatgaaca gcaccgtctt gttcgactgc tgccagatgt
     2821 tcagcagcag ttcctgcatc aatgaacgag tctggctgtc cagcgccccg aatggttcgt
     2881 ccatcaacag cacagcggga tcgttcgcca atgcccgcgc cagtgccacg cgctgcatca
     2941 tgccgccaga aagctgcctc gggtaatgct gctcgaaacc atccagcccc acctgcgcca
     3001 ggtacttgcg cgcaatctgc tgccgctcga ctgctgcgac gccacgcagc ttcagcccga
     3061 actccacatt ctgctgcacg gtcagccagg gaaacaacgt gtacgactgg aacaccatcc
     3121 cacggtctcg ccccggtccg gtcacccgct tggcgtccag ccagatcgcg ccctccgtcg
     3181 gttcgatcag gcccgccacc agccgcagca agctggtctt gccgcatcct gacgggccga
     3241 cgatgaccga gaactcctgc tccgccacgt ccagtgagat attctcgagc gcggtcaccg
     3301 ccgtcccggt cttcggcgta aagcgcaatg ccacattatc gatccgcagc tttgcacccg
     3361 cggtcgcagc accctcgaca tgttgcagca tcacgggctc gtcgcccacg gcatcatcca
     3421 gtttcgcagc gccttgaaga gctggtcggt catgagccca agcaagccga tcaccatgat
     3481 gcccaggaag atctgctcgg tgaacaagcc cagcatcgag ttaagaatga agtag
//
LOCUS       DATGEM010000698        12552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1542699, whole genome shotgun sequence.
ACCESSION   DATGEM010000698 DATGEM010000000
VERSION     DATGEM010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542699"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..836
                     /locus_tag="VKQ27_21430"
     CDS             45..836
                     /locus_tag="VKQ27_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91559.1"
                     /translation="MEATQADVLRLSAEHQLAQAEIARAERLVEDLQHRLASEMAEKE
                     RQLILAESSLRQREEDIARIGQERDEARAEIRDKATQLESVGAILEATQADMLRLSAE
                     HQSAQAEMARGERLVEDLQHRLASEMAEKTQLHDAVSTAVAHAAELMAARERAEQAGR
                     EELARHAEAERIAAEHHQAAIGELTRHQEWLGQINAFLRNRPQWWAIMPAAWQRRHEH
                     AALRKRGLFDAEAYLRLYPDVAADGADPVQHYLTHGMAEGRRLIR"
     gene            complement(1091..1738)
                     /locus_tag="VKQ27_21435"
     CDS             complement(1091..1738)
                     /locus_tag="VKQ27_21435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLY91560.1"
                     /translation="MPHDRSLALAQAIGALSMETFVSQGDGHVAVLRHHGLADGMSVY
                     DLGCGCGRTAQALRRSGWQGHYIGADVVPELIQELALQCPGYKVELNMTPTIVAPDAS
                     LDLVFHWSVFTHLYPSEAYLYTADAFRALKPGGKMIFSFLELEDPAHDRIWESSLRHL
                     RKGHVAEQLDAFLHRDWIRRFARDAGFAEPQFTDGSDEKDHPPFWQALAILEKPI"
     gene            complement(2043..2912)
                     /gene="rfbA"
                     /locus_tag="VKQ27_21440"
     CDS             complement(2043..2912)
                     /gene="rfbA"
                     /locus_tag="VKQ27_21440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HLY91561.1"
                     /translation="MKGIILAGGSGTRLYPMTLATSKQLMSVYDKPMIYYPLTTLMLA
                     GIREVLIISTPRDLPAFRSLLGDGSQWGMTIDYAEQPSPDGLAQAYIIGADFVAGQPS
                     ALVLGDNIYHGHGLPELLASATSRKTGASVFAYHVNDPERYGVVSFDAEMKALSIEEK
                     PERPQSNWAVTGLYFYDEQVLDIAANLKPSARGELEITDVNRVYLERGALSVEIMGRG
                     YAWLDTGTPDSLLDAAEFVRVLEKRQGFKIASPEEIAFRQGFIGAAEMERTIAKLGKS
                     DYGRYLRGVLTNG"
     gene            complement(2927..3814)
                     /gene="rfbD"
                     /locus_tag="VKQ27_21445"
     CDS             complement(2927..3814)
                     /gene="rfbD"
                     /locus_tag="VKQ27_21445"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HLY91562.1"
                     /translation="MRIAVTGRAGQVVTSLIERGAASGHDVIAVGRPELDLADPASVD
                     HALEAAAPDVIVSAAAYTAVDKAESARDLAYAVNGRGAGLVAEAARRLGVPLVHISTD
                     YVFDGRLDRPYIETDPTGPTGVYGVSKLAGEEAVRAAYDDNSAILRVAWVYSPFGANF
                     VRTMLRLAGDRDEVSVVADQLGNPTSALDIADGVLRVAGNLAADPDPALRGLFHMTAE
                     GEASWADFAEAIFAASAAYGGPYAAVRRITTADYPTPAMRPANSRLDSRRIAAIHGVA
                     LPDWRGSLESVVARLRATN"
     gene            complement(3811..4887)
                     /gene="rfbB"
                     /locus_tag="VKQ27_21450"
     CDS             complement(3811..4887)
                     /gene="rfbB"
                     /locus_tag="VKQ27_21450"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HLY91563.1"
                     /translation="MRVIVTGGAGFIGSALVRHLVLDKGHEVLTLDALTYAGCEASLR
                     AVEGKANHRFLKADIRDRAAMDAAIAGFRPDRIMHLAAESHVDRSITGASDFIQTNVV
                     GTFTLLEAARGYWNGLDPQAKADFRFLHVSTDEVYGSLGDEGLFTETTPYDPSSPYSA
                     SKAASDHLAKAWQRTYGLPVVVSNCSNNYGPYHFPEKLIPLTILNALAGRPLPVYGKG
                     ENVRDWLYVNDHARALDLIVERGRPGETYNVGGRNERRNIDVVRRICAVLDTLVPADA
                     PRENLIEFVTDRPGHDARYAIDATRLETELGWQAQENFDSGIEKTVRWYLDNEWWWRP
                     LRDRYDGERLGLSAAAPAVAEAAA"
     gene            complement(4878..5456)
                     /gene="rfbC"
                     /locus_tag="VKQ27_21455"
     CDS             complement(4878..5456)
                     /gene="rfbC"
                     /locus_tag="VKQ27_21455"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HLY91564.1"
                     /translation="MEVESFDIAGPKRFISRHIGDERGYFAETFRADLFAEHVGDFAF
                     VQDNESRSSRAGTVRGLHFQSEPHPQGKLVRCTAGALFDVAVDIRRGSPTYGQWVGET
                     LTPENGRQLWVPPGFAHGFCTLEPNTVISYKVTGYYNAACDKGLAWDDPAIGIAWPAI
                     ADPATLSAKDRQQPRLSELPAYFTWSELVSCA"
     gene            5936..7096
                     /locus_tag="VKQ27_21460"
     CDS             5936..7096
                     /locus_tag="VKQ27_21460"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HLY91565.1"
                     /translation="MNVEKSFRVEMEASVHRSAFSAWLSKRRWFVLFVIVPTIVAAVY
                     YGLIASDVYVSEARFVIKSPDQRRPQLSTLANLIQTTGLSSGQEQATEILEFVRSRDA
                     LKALEKTSAIRNRFAAHGDPFSRFPAPLRGNSFEDLYKYYGKMVEAHLDTESGTAIIT
                     VKAFTADDAYAINRNLLQLSEAMVNKLNARAQTQVVAEAQKQVDLAVERAKRARVALA
                     EYRNAQGLIDPAKQAVGVIEIANNMIAKRAALQAQLDLMQRVTPRNPSIPELRSNINA
                     ISTQIAAQDERVVGSDRSIASKQGDFESRLVEQEFATQNLNVANAALVQARNDAQRQQ
                     FYLERIVDANKPDTPLLPRRLLNIIIVAAVLSFLYFTGWMLIVGILEHAPEG"
     gene            7099..7896
                     /locus_tag="VKQ27_21465"
     CDS             7099..7896
                     /locus_tag="VKQ27_21465"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91566.1"
                     /translation="MSGNTLHRLKNGWRVQVRVIHALMIRELHTRFGRENIGFLWIMV
                     EPLLFAGLVAIIWRFLKGPDEHGMSIIAFVISGYIPITLFRHGVSRSVSIFVANSSLM
                     YHRQVKIIDFVLVRFLIEVLGSMMAYVFIATILILFDQFPLPSDPGLFVAGWTLYSLF
                     TLSLCFMIAPLSELSEILEKFIPVTTYVMVPFSGLFTMASWLSPKMREYLLWSPFVNA
                     MEMMRKGIWGDQITAYYNIWNPLGCSLVATVLGLALCRKVRKTMTVE"
     gene            7860..8540
                     /locus_tag="VKQ27_21470"
     CDS             7860..8540
                     /locus_tag="VKQ27_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91567.1"
                     /translation="MPQSTQDDDRRMIVCKNISKSYKHGAARKQVLDNVNFVIGRGDR
                     VALLGRNGAGKTTLIKLIGGVEMPTSGRITRGMSISWPLGFAGGFQGSLTGYDNARFI
                     ARIYGHDYKDMKAFVEDFTELGRQLAMPVKTYSSGMRARLAFALSLAIEFDCYLIDEI
                     ILVGDQNFQRKCQHELFEKRKDRTMILASHSIEVVRQFCNKAFIIHQGRVTAYDDVDH
                     AVDLYSAL"
     gene            complement(8611..9528)
                     /locus_tag="VKQ27_21475"
     CDS             complement(8611..9528)
                     /locus_tag="VKQ27_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91568.1"
                     /translation="MREPVRAQGYGFDMQQDQARLIGAEQNNNHIMGDDAHLAGQVAT
                     LESTLRQRQEEIEQAWTRAEELATERNELQRRIDELQTAQERLETRLTKADEWVFELA
                     GERRSLEDRLAAVQSEVVKARRSITGMNVVSEHKQKQLLEQREAMTLLLEQKVALHRE
                     VEMLLERLEDRERAARDVAALRSDALEYHAKAARALEQLDIERAENKQRIRSLRAMEG
                     RATQKDRDLDWMRRLYQLLDKSASGWRGLLPPMMRRRRVARMLRQNGLFDADAYLAKY
                     ADVAESGMDPLRHYVLHGMAEGRFTDSQV"
     gene            complement(9865..11181)
                     /locus_tag="VKQ27_21480"
     CDS             complement(9865..11181)
                     /locus_tag="VKQ27_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HLY91569.1"
                     /translation="MLLASRQIGLSSNVSGHDLPAPDLVVTGRVAGLGLTASKASGAD
                     SCVGGTRAVASLDLRVRDARSGATLFGGNVTKSVALSSNIVAGGGRCSTLTPDDASYD
                     RVEGALALTAARAVLFHIAPLRVTGISGRHIALNYGTPILTLGTRAQVTGAGGFPLRY
                     QVVAATAEAAFAEPDGEAGPVDTLKLRIPERSLNNADSSRQKIGLNSVSQLADQSDIE
                     RQADAFTHPIDFDVQPTDDGSKVFAETENGNPMFDFAAEVIASDLLRSTIENAQRQQQ
                     LEQDQQQAAQTAAQAAQRVTGLTAAKADDQLANPTIDAQWKALPRPLRAQLLAAQRAW
                     SAKKDADCRVQAAAASIDPSEMEAARLTCDARSHGLAPTAGRERCNAIRRRGQPPRHA
                     PAVPPAGFLAGEALNRRRTNGEHSSPLRRLRRSTGFHSPPCSATDG"
     gene            complement(11389..12066)
                     /locus_tag="VKQ27_21485"
     CDS             complement(11389..12066)
                     /locus_tag="VKQ27_21485"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HLY91570.1"
                     /translation="MGYPPVEQARAAGGMVTTNTPSKVGGFEGIDNLVYGTITSVSAK
                     RSGDVGSTLMAGLLGQRDAHCTAVKATLALDIKITDSRTGEIRYATHIDDSQKSVPNC
                     SGNEKIDTAALMRSAADKVANGLTNCIYPIQIAMAAPDGSLVQLWRGHRDAGGADGGL
                     FQGRSDPRSGLGRHHRQHRNQARADPRERCGGAHVEGHACHGVQHDAPGGLDRAPRKR
                     RGCQGAR"
BASE COUNT         2257 a         3924 c         3967 g         2404 t
ORIGIN      
        1 agatccgggg caaagccacc caactggaat ccctcggtgc cacattggag gccacccagg
       61 cagacgtgct tcgcctctcg gcggaacatc aattggcaca agcggagatc gcgcgcgcgg
      121 aacgcctggt cgaggatctg caacaccggc tggcttcgga aatggcggaa aaggagcgcc
      181 agctcatact tgctgagagc agccttcgcc agcgtgagga ggatatcgct cggatcggcc
      241 aggagaggga cgaggcgcgg gccgagattc gggacaaagc cacccaactg gaatccgtcg
      301 gtgccatatt ggaggcaacc caggcagaca tgcttcgcct ctcggcggaa catcaatcgg
      361 cacaagcgga gatggcacgc ggggagcgcc tggtcgagga tctgcaacac cggctggctt
      421 cggaaatggc ggaaaaaacg caactgcacg atgccgtctc gaccgccgta gcccatgccg
      481 ctgagctcat ggcggcccgc gagagggcgg agcaggctgg ccgcgaggaa ctggcccggc
      541 acgccgaagc agaacgcatc gctgcggaac atcatcaggc tgcgataggc gaactgaccc
      601 gacatcagga atggctgggg cagatcaacg cgtttctccg aaatcgtccg cagtggtggg
      661 ctatcatgcc cgccgcctgg caacggcgac atgagcacgc cgccctgagg aagcgcggcc
      721 tgttcgatgc ggaagcctat ctgagacttt acccggacgt tgcggcagac ggcgcggatc
      781 cggtgcagca ttatctgaca catgggatgg ccgaggggcg cagactgatt cgctgaccgg
      841 ggcaggggcg atgaagcagg aataggcgct tccggcttgt cggatcggat cgcggcccgc
      901 cgccaattcg gaagggcgcc cgatccgata cgtcactggg ttacccggcg gcgcgcctat
      961 cgcacgctcg acgattatcc tctgccccgc gccgccttct cggtcaggat gccaaccgtc
     1021 cttagcgtac gctggtgaag agattctacg atgacccagg ctcccccttg tcttcttgcg
     1081 ttacagaaga tcagatcggt ttttcgagaa tggcgagtgc ttgccagaag ggaggatgat
     1141 ccttttcgtc cgatccatcg gtgaattgcg gttcggcaaa tccggcatcg cgggcgaagc
     1201 ggcggatcca gtcgcggtgc aggaaggcat caagctgttc ggcgacgtgc cccttgcgca
     1261 gatgtcttag actggattcc caaatccgat cgtgcgccgg atcttccaat tccaggaagg
     1321 agaagatcat cttgccgccc ggcttcagcg cccggaaggc gtccgccgta tagagatagg
     1381 cctcgctcgg atagagatgc gtgaagaccg accagtggaa cacgagatcc agcgaggcat
     1441 cgggcgccac gatcgtcggc gtcatattga gctcgacttt atagcccggg cattgcagcg
     1501 cgagttcctg gatcagctcc ggcaccacgt ccgcgccaat atagtggcct tgccagcccg
     1561 atcgccgcag ggcctgcgcc gtgcgcccgc agccgcagcc gagatcgtac acggacatgc
     1621 catcggcgag gccgtgatgg cgcagcacgg cgacatgccc gtcgccctgc gacacgaatg
     1681 tctccatcga gagcgcgccg atcgcctgcg ccagcgccag cgagcgatca tggggcatct
     1741 cgcgtagttt cttccgggtc agcttcctgt aatcgcgaat ggaatgactc tgatggcgcc
     1801 tcttcggccc gaggcgcgga aaccacaaca tagaccaacc cccaacacgc atgacgaaga
     1861 ccaaccttca gcataccgcc caggacttgg caatctggcc catgaaaaga agcatttgtc
     1921 tgctcacgcg taccgaacgt ctgcatcaac cagcctcatt tacgaggctg atcgtgcccg
     1981 ttcttgccat gatgttcggc aagcggcggc gccagcgggg aggggggtgc cgttcgccgc
     2041 agttacccgt tcgtcaggac gccccgcaga tagcgcccat agtccgactt gccgagcttc
     2101 gcgatggtcc gctccatctc ggccgcgccg atgaagccct ggcggaacgc gatctcctcg
     2161 gggctggcga tcttgaagcc ttggcgcttc tccagcacgc gcacgaactc ggcggcgtcg
     2221 agcaagctgt cgggcgtgcc cgtgtccagc caggcgtaac cgcggcccat gatctcgacc
     2281 gacagcgcgc cgcgctccag atagacgcgg ttgacgtcgg tgatctcgag ttcgccgcgc
     2341 gccgagggct tcaggttggc ggcgatgtcc agcacctgtt cgtcatagaa atagaggccc
     2401 gtcaccgccc agttggactg cggccgctcc ggcttctcct cgatagagag cgccttcatc
     2461 tcggcgtcga agctgaccac gccgtagcgc tcgggatcgt tgacatgata ggcgaagacg
     2521 ctcgcgcccg tcttgcgcga ggtggcgctg gcgagcagct cgggcaggcc gtggccgtgg
     2581 tagatattgt cgcccagcac cagcgccgag ggctggccgg ccacgaaatc ggcgccgatg
     2641 atataagcct gcgcgaggcc gtcggggctc ggctgctcgg catagtcgat ggtcatgccc
     2701 cattgcgacc cgtcgccgag cagcgaccgg aaggcgggca ggtcgcgcgg cgtcgagatg
     2761 atcagaacct cgcggatccc cgccagcatc agcgtggtca gcggataata gatcatcggc
     2821 ttgtcataga ccgacatcag ctgcttggag gtggccagcg tcattggata gaggcgggtg
     2881 ccgctgccgc cggcgaggat gatgcccttc attccctgat ggtcctttag ttggtggccc
     2941 ggagacgggc gacgacgctt tccagcgaac cccgccaatc ggggagcgcg acgccgtgga
     3001 tggctgcgat gcgacggctg tcgagccgcg aattggccgg ccgcatggct ggcgtcgggt
     3061 agtcggcggt cgtgatgcgg cggacagccg catatggtcc gccatacgcg gccgatgccg
     3121 cgaagatcgc ctcggcgaaa tcggcccagc tcgcttcacc ctcggcggtc atatggaaaa
     3181 ggccacgcaa cgccggatcg gggtcggcgg cgagattccc cgccacgcgg agaacgccat
     3241 cggcgatatc gagcgcgctc gtgggattgc cgagctgatc ggcgacgacc gacacctcgt
     3301 cgcggtctcc ggcgagacgc agcatcgtcc tgacaaagtt cgcgccgaac gggctgtaca
     3361 cccaggccac gcgcaggatc gcgctgttgt cgtcataggc cgcgcgcacc gcctcctcgc
     3421 cggcgagctt ggaaactcca tagacgccgg tcggcccggt cgggtcggtc tcgatatagg
     3481 ggcggtcgag ccggccgtcg aagacatagt cggtcgagat atggaccagc ggcacgccca
     3541 gcctccgtgc cgcctcggcg accagccccg cgccccggcc gttgacggca taagcgagat
     3601 cgcgcgcgct ctccgccttg tcgaccgccg tgtaggctgc ggccgagacg atcacgtccg
     3661 gggcggcggc ttcgagcgcg tggtcgaccg aggccggatc ggcgaggtcc agctcgggcc
     3721 ggccgaccgc gatcacgtca tgtccgctgg ccgcgccgcg ctcgatcagg ctggtgacga
     3781 cctggccggc gcggcccgtg acagcgatcc tcacgcggcc gcctcggcga cggcgggggc
     3841 ggccgccgaa agccccagcc gctcgccatc atagcgatcg cgcaaggggc gccaccacca
     3901 ttcattgtca agataccagc gcaccgtctt ctcgatgccg ctgtcgaaat tctcctgcgc
     3961 ctgccagccg agctcggttt caagccgggt cgcgtcgatc gcgtagcgcg cgtcatggcc
     4021 ggggcgatcc gtcacgaact cgatcaggtt ttcgcgcggg gcgtcggcgg ggaccagcgt
     4081 gtcgagcacc gcgcagatac ggcgcacgac atcgatgttg cgccgctcgt tgcgtccgcc
     4141 gacattgtag gtctcgcccg gtcgcccgcg ttcgacgatg agatccagcg cgcgggcgtg
     4201 atcgttgaca taaagccagt cgcgcacatt ctcgcccttg ccatagacgg gtagcgggcg
     4261 ccccgcgagc gcgttgagga tggtcagcgg gatcagcttt tcggggaaat gatagggccc
     4321 gtaattgttc gagcagttcg acaccaccac gggcagcccg taggtgcgct gccacgcctt
     4381 ggcgagatga tcggacgccg ccttggaggc cgaatagggc gagctcgggt catagggcgt
     4441 ggtttcggtg aacagtccct cgtcgcccag cgagccgtaa acctcgtcgg tcgagacgtg
     4501 gaggaagcgg aaatccgctt tggcctgggg atcgagcccg ttccagtagc cgcgcgccgc
     4561 ctcgagcagg gtgaaggtgc cgacgacgtt tgtctggatg aaatcggacg cgcccgtgat
     4621 cgagcgatcg acatggctct cggcggcgag atgcatgatc cggtcggggc ggaagccggc
     4681 gatcgcggcg tccatcgcgg cgcggtcgcg gatatcggcc ttgaggaagc gatggttggc
     4741 cttgccctcg accgcgcgca gcgacgcctc gcagccggca taggtgaggg cgtcgagggt
     4801 gagcacctca tggcccttgt cgagcacgag atggcgcacg agggccgaac cgatgaagcc
     4861 ggcaccgccg gtgacgatta cgcgcatgag acgagttcac tccaggtgaa ataggcggga
     4921 agctcggaaa ggcggggctg ctggcgatcc ttggccgaga gggtggccgg atcggcgatg
     4981 gcgggccagg cgatgccgat cgccggatcg tcccacgcca gccccttgtc gcacgcggcg
     5041 ttgtaatagc cggtgacctt ataggagatg accgtattcg gctcgagcgt gcagaaaccg
     5101 tgcgcgaagc cgggcggcac ccagagctgc ctgccgttct cgggcgtcag cgtctcgccg
     5161 acccactggc cgtaggtggg cgatccccgc cggatatcga ccgcgacatc gaacagcgcg
     5221 ccggcggtgc agcgcacgag cttgccctgc gggtggggtt cggactggaa atgcagaccg
     5281 cggacagtgc ccgcgcgcga gctgcgggac tcgttgtcct gcacgaaggc gaagtcgccg
     5341 acatgctcgg cgaagagatc ggcccggaac gtctccgcga aatagccccg ctcgtcgccg
     5401 atatggcgac taataaaacg cttcggacct gcaatatcga aggattctac ttccatcttc
     5461 gctctcttag aaacggcatc atttcgcatt cgaaattcac aacgaaattc cgggcgagat
     5521 attcaacctt ccctctgcgc tggcctttcc gttccgttcg cacacgagag agttcgacgg
     5581 aagcgaggtg cggccggcaa tgcttccaaa cggaatcggg ccatcgaata ttttgcgctt
     5641 catcgctgga tgacctcagg ggctttcagc ttcaatggct gtgcccttag catcgcatat
     5701 cgggaatgga caataccgcg cggcgagtgc gcgtgccgca tactcctcat ccggccgatt
     5761 cacggattta gcctcgataa cagcttgtcg cggcttcggc tgtcctgcgg ggcgctgctg
     5821 tcgggcaatc caacgcgacc cacttgcgtg ctgttgtagc ttggcttagt gcgcccatga
     5881 tggccgagca tcagccctcg ggcgatctta ttgtttctcc ggccatgaag gtcttatgaa
     5941 cgtcgaaaaa tcgttccggg ttgagatgga agcatcggtc caccgatccg cattctcagc
     6001 atggctcagt aaacggcgct ggtttgtcct gttcgtgatt gtgccgacga tcgttgcggc
     6061 ggtctattac gggctgattg cgtccgacgt ctatgtttcc gaggcgcgat tcgtcatcaa
     6121 gagccccgat cagaggcgcc cccagctttc taccctggcc aatctgatcc agacaacagg
     6181 gctgtcgagc gggcaggaac aggcgactga gattctcgaa ttcgtgcgtt cgcgcgatgc
     6241 gctcaaggcg ctcgagaaga cttccgccat tcgcaacagg ttcgccgcgc acggcgatcc
     6301 cttctcgcgg ttccccgctc ctttgcgcgg taactccttc gaggacttgt acaaatatta
     6361 cggcaagatg gtggaggccc atctcgacac cgagagcggc accgccatca tcacggtcaa
     6421 ggccttcact gccgatgacg catatgccat caatcgtaat ctgctccagc tcagcgaggc
     6481 gatggtcaac aagctcaatg cccgagccca gacccaagtg gtcgcggagg cgcagaagca
     6541 ggtggatctc gcggtggagc gggcgaagcg cgcgcgcgtc gcgcttgcgg aatatcgcaa
     6601 cgcccagggg ctcatcgatc cggccaaaca ggcggtcggg gtgattgaaa tcgctaacaa
     6661 catgatcgcc aagcgcgccg cgctccaggc ccagctcgat ctgatgcagc gcgtgacgcc
     6721 ccgaaacccg tcaatccccg agttgcgcag caatatcaac gcgatctcca cccagatcgc
     6781 tgcgcaagat gagcgtgtgg ttggctccga ccggagcatc gcatccaagc agggcgactt
     6841 tgaaagccgc ctcgtcgagc aggaattcgc gacgcaaaat ctcaacgtgg ccaatgcggc
     6901 gctcgttcag gcccgcaacg atgcccagcg ccagcaattc tatctcgaac gcatcgttga
     6961 tgccaacaag cccgatactc cgctactgcc gcgtcggctg cttaatatca tcatagtcgc
     7021 tgcggttctc agcttcctct acttcacggg atggatgctc atcgtcggga ttctcgaaca
     7081 cgcgccggaa ggctgataat gtccggtaat acccttcata ggttaaagaa tgggtggcgc
     7141 gtccaggtac gggtgatcca cgccttgatg atccgcgagc tccatacccg gtttgggcgg
     7201 gagaatatcg gtttcctgtg gatcatggtc gagcccctgc tgttcgccgg cctggtggcc
     7261 attatctggc gcttcctgaa ggggccggat gaacatggca tgagcatcat cgcgtttgtt
     7321 atcagcggct atattccgat caccttgttt cggcatggcg tatcgcggag tgtcagcatc
     7381 ttcgttgcca acagtagctt gatgtatcat cgacaagtaa aaattatcga ttttgttctt
     7441 gtgcggtttt tgatcgaagt tttaggtagc atgatggctt acgtcttcat cgcgaccatc
     7501 ctgatacttt tcgatcaatt cccgttgcct tccgatcctg gcctctttgt cgcgggttgg
     7561 acgctctatt cgttatttac gttgtcgctg tgcttcatga tcgctccgct ttcggaactg
     7621 tctgaaatac tcgagaaatt tatcccggtg acgacatatg tcatggtccc gttttcgggg
     7681 ctgttcacga tggcttcgtg gctcagccca aagatgcgcg agtatttgct gtggtcaccc
     7741 ttcgtcaatg cgatggagat gatgcgtaag ggtatttggg gcgaccagat tacggcctat
     7801 tacaatatct ggaatccgct cggctgttca ctcgtggcga ccgtactcgg cctcgccctg
     7861 tgccgcaaag tacgcaagac gatgaccgtc gaatgatcgt ctgcaaaaac atttcaaagt
     7921 catacaagca cggagctgcg cgaaagcagg ttctggacaa cgtcaacttt gtcatcggac
     7981 ggggcgaccg ggtcgcgttg ctgggccgca acggcgcagg caagaccacg ttgatcaagc
     8041 tgattggtgg cgtggaaatg ccaacctccg gccggatcac gcgcggcatg tcgatttcct
     8101 ggccgctggg gtttgccggc ggctttcaag gcagtctgac cggctatgac aatgctcgct
     8161 tcatcgcgcg gatatacggc cacgattaca aagacatgaa ggcattcgtc gaagacttca
     8221 ccgaattggg gcgccagctt gcgatgccgg tcaagaccta ttcttcgggt atgcgggcgc
     8281 gccttgcctt cgcgctatcg cttgcgatcg agttcgactg ctatttgatc gacgagatca
     8341 tcctggtcgg cgatcagaat ttccagcgca aatgccaaca tgaactgttc gagaagcgca
     8401 aggaccgcac catgatccta gcatcgcaca gcatcgaagt ggtgcgccag ttctgcaaca
     8461 aggcgttcat catccaccag ggacgggtga ccgcctatga tgacgtcgac cacgccgtgg
     8521 atctgtacag cgcgctttga tatcatccga ccgacggcca gcgtttgtgc gggcctgcgt
     8581 cgccccccaa acgctgactg ctggcgccgg tcagacctgg ctgtcggtga agcgaccttc
     8641 cgccattccg tgcagaacat agtggcgcag cggatccatg cccgattcag cgacatctgc
     8701 gtatttggcc aaataggcat cggcatcgaa gagcccattc tgccgtaaca tgcgcgccac
     8761 gcgccgtcga cgcatcatcg gcggaagaag gcctctccaa ccggatgcgc tcttgtcgag
     8821 gagttgatag agccgccgca tccagtcgag gtcgcgatcc ttttgggtgg cgcggccctc
     8881 catggcgcgc agggagcgga tccgctgctt gttttcggcg cgctctatat cgagctgttc
     8941 cagggcccgc gcggccttgg catgatattc cagcgcgtcg gaacgcaggg cggccacgtc
     9001 gcgcgcggcg cgttcacgat cctccagccg ttccaggagc atctcgacct cgcgatgcag
     9061 cgcgaccttt tgttcaagga gtagagtcat ggcctcgcgc tgttcaagaa gctgcttttg
     9121 cttgtgttcg gaaacgacat tcatccctgt gatcgatcgc cgtgccttca cgacttccga
     9181 ttggaccgcg gccagccgat cttcgagcga gcggcgttcg ccggccagct caaacaccca
     9241 ttcatcagcc ttcgtgaggc gagtctcgag acgctcctgc gctgtctgca gttcgtcgat
     9301 gcggcgctga agctcgttgc gctcggtcgc gagttcctcc gcgcgcgtcc aggcctgctc
     9361 aatctcctcc tgacgttgac gcagggtgct ttcgagcgtt gccacctggc ctgcgagatg
     9421 cgcgtcatcc cccatgatgt ggttgttgtt ctgctccgcc ccaatgaggc gcgcctgatc
     9481 ctgctgcata tcgaagccgt aaccttgtgc cctgacgggt tcgcgcactg ccgacgcgtt
     9541 ccgtcgatga gcccttatct ttagtgccat cgctctcgtt gtaaattgca cctgattgcc
     9601 aagtgcctac attgagagac ggatggctat tgtaaacgaa ggcggtattc aatggctcgg
     9661 cactcggccc tttgcggaaa atcccgacga aatcgggggc atcgcaaaat ctggtcgaaa
     9721 acgtcgatca tgcccgtacg ctcgccacgg ctcctcttcc gcgccgcccc cttcggcatc
     9781 ggccagcccg aagaatttgc gccgggctgc gcgaagcaac cagcctcgag cacagcgctc
     9841 ggcctagcgg gtgagcggct ggtgctaccc gtccgttgcc gaacatggcg gcgagtggaa
     9901 cccggtgctg cgcctcagcc ggcgaagggg ggatgaatgc tcgccgttcg tccggcggcg
     9961 gttcagggct tctccggcta gaaatcctgc tggggggacg gctggcgcat gtcggggcgg
    10021 ctgtccgcgg cggcggatgg cattgcatcg ctcgcgccct gctgttggag ccaggccgtg
    10081 cgatcgcgcg tcgcaggtga ggcgcgcggc ctccatctcg ctcggatcga tcgaggcggc
    10141 ggccgcctgc acgcggcagt ccgcatcctt cttggccgac caggcgcgct gcgccgccag
    10201 caactgcgcg cgcagcgggc gcggcaacgc cttccactgc gcgtcgatcg tcgggttggc
    10261 gagctggtcg tcggctttgg cggcggtcag cccggtgact cgctgcgcgg cctgcgctgc
    10321 ggtctgggcg gcctgctgct ggtcctgctc gagctgctgc tggcgctggg cgttctcgat
    10381 cgtcgagcgc agcagatcgg acgcgatcac ttccgccgcg aagtcgaaca tcggattgcc
    10441 gttctccgtc tcggcgaaga ccttgctgcc gtcgtcggtc ggctggacgt cgaagtcgat
    10501 cggatgggtg aaggcgtcgg cctggcgctc gatgtcgctc tggtcggcga gctgtgaaac
    10561 gctgttcagg ccgatcttct gccggctgga atcggcattg ttcaggctgc gttcggggat
    10621 gcgcagcttc agcgtgtcga ccggcccggc ctctccgtcg ggttcggcga aggcggcctc
    10681 ggccgtggcg gcgaccacct ggtagcgcag cgggaaaccg ccggcgcccg tcacctgcgc
    10741 cctcgtcccc agcgtcagga tcggcgtgcc ataattgagc gcgatgtgcc ggccgctgat
    10801 ccccgtcacg cgcagcggcg cgatatggaa aagcacggca cgcgccgccg tcagcgcgag
    10861 cgccccctcg acccggtcgt agctggcatc gtccggcgtc agcgtgctgc agcgccctcc
    10921 gcccgcgacg atattcgacg aaagtgccac ggattttgtg acattgccgc cgaacagcgt
    10981 cgcgccgctg cgcgcatcgc ggaccctgag atcgaggctg gcgaccgcgc gcgtgccgcc
    11041 gacgcaagag tccgcgccgc tcgccttcga ggcggtgagc ccgaggccgg ccacgcgccc
    11101 cgtcaccacc aggtcgggcg cgggcagatc atggcccgag acgttcgacg acaggccgat
    11161 ctgcctgctg gcgagcagca tcgactggag gttggcccgc agatcgggga atgcgcgcgg
    11221 cactgggtgg cgagccgcca gccccagccg acataggtgg cgcaatcctg cgcgctctgg
    11281 atcttgccga ccttgacgat cggcacggcc gcgcctgccg gcgtgccgat cagcccgccg
    11341 agcagcgccg ccccccgcaa ggatccgctt catttgcgtc ctgcggcctt atcgagcgcc
    11401 ttggcatcct cggcgcttgc gaggcgcacg atcgagccca ccgggggcgt catgctgaac
    11461 gccgtgacag gcgtggcctt cgacatgcgc gccgccacat cgctcacgcg gatcagcccg
    11521 agcttggttt cggtgctgcc gatgatgtcg cccgaggccg gatcgcggat cggatcgccc
    11581 ttggaataga ccgccatcag cgccccccgc gtcacggtgc cctcgccata gttggaccag
    11641 cgatccgtcg ggcgctgcca tcgcgatctg gatcggatag atgcagttgg tcaggccgtt
    11701 ggcgaccttg tcggccgcgg agcgcatcag cgccgcggta tcgatcttct cgttgccgct
    11761 gcagttcggc accgacttct ggctgtcgtc gatatgcgtc gcataccgga tctcgccggt
    11821 gcgggagtcg gtgatcttga tgtctagcgc cagcgtcgcc ttgaccgccg tgcagtgcgc
    11881 atcgcgctgg ccgagtaagc cggccatcag cgtcgagccg acatcgccgc tgcgcttggc
    11941 ggaaaccgag gtgatcgtgc catagacgag attgtcgata ccctcgaacc cgccgacctt
    12001 gctgggcgtg ttggttgtga ccatgccgcc ggccgcgcgg gcctgttcga ccggcggata
    12061 ccccatccac ctgtcgccag ttgactaacg ctgttcacgg caccccgcaa tccgggaaaa
    12121 ccccggattg gatcgctatc ctgatggact cacagggaat tctcgagcct ttgactggcc
    12181 accacagcct aactgcgccg cttgtcgttt aatagtccct gtcgacgggc tataaaatcg
    12241 aaaacacaca acagaattgg cagtgccctt tgccgttacc gctgaacttc gatcgcttca
    12301 tcgtacgcta cgctttctgg ctcgtcgatc acaactggaa tttttttcta ggtcaaaacg
    12361 gtccaaaaaa acgggaggca aatcagtcgg atcgaccttg ataaatgaga aaaattgcgc
    12421 atcatctact attaatgtct cttgaatggt atctttctat ctaaatttgg ggagggcgat
    12481 tccgtgacat atatagatag cgtgctattc aatcccggca ttggtctaga ttggcatatg
    12541 cagaatggag ag
//
LOCUS       DATGEM010000699         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_846003, whole genome shotgun sequence.
ACCESSION   DATGEM010000699 DATGEM010000000
VERSION     DATGEM010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846003"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VKQ27_21490"
     CDS             complement(<1..332)
                     /locus_tag="VKQ27_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLY91571.1"
                     /translation="MERYDLLVVGGGINGCGIARDAAGRGLSVLLVEQDDLANHTSSA
                     STKLIHGGLRYLEYYEFRLVREALIERERLLAIAPHIIWPLRFVMPLPPGGRPAWMIR
                     LGLFLYDHL"
     gene            complement(799..2070)
                     /locus_tag="VKQ27_21495"
     CDS             complement(799..2070)
                     /locus_tag="VKQ27_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HLY91572.1"
                     /translation="MLTTGVFAQGMVPPAQATPTTIAAQSAEDPAIRPFHVHVAQKNI
                     DELRRRIRETRWPDRETVDDQSQGIKLAKLKPLVDYWGTHYDWRKAEAKLNAYPQFVT
                     KIDGVDIHFIHVRSKNPNALPVIITHGWPGSVFEELKVIGLLTNPTAYGGRAEDAFDV
                     VIPSMPGYGFSGKPTEAGWGPDRIARTWAELMKRLGYARYVAQGGDWGSPVSSAMARQ
                     APPGLLGIHINLPAVVPPDVGAALAAGGPAPAGLSGTERAAYDALNTYAKKGSRSYAV
                     IMGTRPQTIGYGLSDSPAGLAAWMLGHPGFTNWTYDGSDPEKSPDDVLDDISLYWLTN
                     SATSAARLYWEAGSTSPVLSAPQHTTDIALPVAITVFPGEVYRAPESWARRAYPKLTY
                     FHEVDKGGHFAAWEEPTLFASELRAAFRPLR"
     gene            complement(2121..2759)
                     /locus_tag="VKQ27_21500"
     CDS             complement(2121..2759)
                     /locus_tag="VKQ27_21500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLY91573.1"
                     /translation="MSQPLYSKESTALLLVDPYNDFLSEGGKAWPHLKPVAEEVGLLG
                     HLRALDHAARAAGIQVAIAPHKRWQPGDYEDWAHPNPSQQKIMQGHHFAHGEWGGDWH
                     PDLAPKPGDIVAKEHWGQSGFANTDLDFRLRQKGITHVIVVGLLANTCIESTARYAME
                     LGYHVTLVRDATAAFAPELMHAAHELNGPTFAHMILTTAELLAALPAAGPEA"
     gene            complement(2798..3322)
                     /locus_tag="VKQ27_21505"
     CDS             complement(2798..3322)
                     /locus_tag="VKQ27_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91574.1"
                     /translation="MPGPEIVDSSVTAIINAPIEKVDLPTWAFTLPEHEYQGCSPAHI
                     AAGFTTAPDGERMSINVEIIGGVLMVQHYVEALGQKNHLILESVSDLFTPTGRTTIHV
                     LWELSVRAIDGERCEFTNHVRSSATPEFKAFLDRQGIPFDVFRAQRQPMTIAHNEGET
                     PLFAASIERAALRN"
BASE COUNT          608 a         1081 c         1186 g          647 t
ORIGIN      
        1 agatgatcgt aaaggaacag gcccaggcgg atcatccagg ccggccggcc gccgggcgga
       61 agcggcatca cgaagcgcag cggccagatg atatggggtg cgatcgccag cagccgctct
      121 cgctcgatca gcgcttcgcg gaccagccgg aattcataat attcgagata gcgcaggccg
      181 ccgtggatga gcttggtcga cgccgacgag gtgtggttgg cgagatcatc ctgctcgacg
      241 agtagcaccg acaagccgcg gcccgccgcg tcacgggcga taccgcatcc attgatcccc
      301 ccgcccacta cgagcagatc gtaccgctcc atatttttat ccacttatct gtctcccgtt
      361 gtcatatcgg gcgacgtggc tttccgtcaa tgcggggcga acgacctctc acgactccat
      421 atcacgtagc cgggcatttg ctcggagaga agggggcgat cgttcccgat gcgatccagt
      481 cagagactga cacccgctta cctcgtcaga gcgcgcgatc gaaccgctgt ccgcgagcag
      541 ccacccatca cgggccgcag gagacgatca tgcctgacgc tacccaccgc cgcggcgcgg
      601 ggcgcatggg ttgcgccgga agatgccagc tgcggcgatg ccatgcgcat gcggccggga
      661 cgtcacagca ccccgaccaa cgcgtcacgg ctcggattcc cgggatcgcg ccgctgtgta
      721 gggccaccac gccacggcgc tccagcgcct tgaggcagcg attgaccttc ggccggctcg
      781 cgccgaccgc gtcgccgatc acctcagggg ccggaatgca gctcgcagct cggaggcgaa
      841 gagcgtcggt tcctcccaag cggcgaagtg gccccccttg tcgacctcgt ggaaataggt
      901 gagcttggga taggcgcgcc gcgcccatga ctcaggcgca cgatagactt ccccagggaa
      961 gaccgtgatt gcgaccggga gcgcgatgtc ggtggtatgc tgtggggccg acaggacggg
     1021 gctggtgctt ccggcctccc agtaaagccg ggccgccgag gtcgcgctgt tcgtcagcca
     1081 atagagcgag atgtcatcca gtacgtcatc cggagacttt tcgggatcgc tgccgtcata
     1141 ggtccagttg gtgaagcccg gatgcccgag catccacgcc gcaagcccgg ccggcgagtc
     1201 cgacaggcca tagccgatcg tctgcgggcg cgtgcccatg atgaccgcgt aggatcggct
     1261 gcccttcttg gcataggtgt tgagtgcgtc gtatgccgcg cgttccgtgc ccgagagccc
     1321 cgccggcgcg gggccaccgg cggcgagcgc cgctcccaca tcgggcggca caaccgccgg
     1381 gaggttgatg tgaattccga gcaggcccgg gggggcctga cgcgccatcg cgcttgaaac
     1441 gggcgaaccc cagtcgccgc cctgcgcgac gtaacgcgcg tagccgaggc gtttcatcag
     1501 ctcggcccag gtgcgcgcga tccggtcggg gccccaaccg gcttcggtcg gtttgccgga
     1561 gaagccatag cccggcatcg acgggataac cacgtcgaac gcgtcctccg cacgcccgcc
     1621 ataggccgtg ggattggtga gcaggccgat gaccttgagt tcctcgaaca ccgatcccgg
     1681 ccagccatgc gtgatgatca ccggcagcgc gttggggttc ttcgaacgga cgtggatgaa
     1741 gtggatgtcg acgccgtcga tcttcgttac gaactgggga taggcgttga gcttcgcttc
     1801 cgcctttcgc cagtcataat gtgtccccca atagtcgacg agcggtttga gcttcgcgag
     1861 cttgatgccc tgcgactggt cgtcgaccgt ctcccgatcg ggccagcggg tttcgcggat
     1921 gcggcgcctg agctcgtcga tattcttctg ggcgacgtgg acgtggaagg ggcggatcgc
     1981 tgggtcttcg gccgattggg cggcaatcgt cgtcggagtg gcttgggccg gcgggaccat
     2041 gccttgtgcg aagacgcctg tggtgagcat ggcgccggcc ccgagtgccg caagcgttct
     2101 gatcaagggg ggcatgggtt tcacgcctcg gggccagctg cgggcaaggc cgccagaagc
     2161 tcggcggtcg tcaggatcat atgggcgaag gtcgggccgt tcaattcgtg cgcggcgtgc
     2221 atcagttccg gcgcgaaagc ggcagtggcg tcacgcacca gcgtcacatg atagccgagc
     2281 tccatcgcgt agcgggcggt ggactcgatg caggtgttgg cgagtagccc gaccacgatg
     2341 acgtgggtga tccccttctg tctcagtcga aagtcgagat cggtattggc gaagccgctc
     2401 tgcccccaat gctccttggc gacgatgtcg ccgggcttgg gcgccaaatc gggatgccag
     2461 tcgccgcccc attcgccgtg cgcgaaatga tggccctgca tgatcttctg ctgcgaaggg
     2521 ttgggatgag cccagtcctc atagtcgccc ggctgccagc gtttgtgcgg ggcaatcgcc
     2581 acctggatgc cagcggcgcg ggccgcgtgg tccagcgccc ggaggtggcc gaggagcccg
     2641 acctcctccg ccaccggctt gaggtgcggc caggccttcc cgccttcgga caggaagtcg
     2701 ttataggggt cgaccagaag gagtgcggtc gactccttac tgtagagggg ctgtgacatg
     2761 tcgtgtctcc gaaatcgtat ttgcgggccg gcggcgatca gttgcgcagg gcagcccgct
     2821 cgatgcttgc ggcgaacagc ggcgtttcac cctcgttatg ggcgatcgtc atgggctgac
     2881 gttgcgcccg gaacacgtcg aaggggatgc cctgccgatc gaggaaggcc ttgaactccg
     2941 gcgtggcact ggatcggaca tggttggtga attcacaccg ctcaccgtcg atggccctga
     3001 cactcagttc ccaaagcaca tggatcgtgg ttcggccggt gggcgtgaaa aggtcggaga
     3061 ccgactccag gatcaggtgg ttcttctggc cgagggcttc gacataatgc tggaccatca
     3121 ggacaccccc gatgatctcg acattgatcg acatccgctc gccgtcgggc gccgtcgtga
     3181 agcctgcggc gatgtgcgca ggcgagcagc cctgatattc atgctcgggc agcgtgaagg
     3241 cccaggtcgg aagatcgacc ttctcgatcg gtgcgttgat gatcgcggtg acgctggaat
     3301 cgacgatctc ggggccgggc atattgatct ccttgggttt tcgatggagc cggcggctgc
     3361 acggccgttg catcggctcc aacagtgctt attttactat gaaaaccata gtttcaagtg
     3421 cgtatgaaaa agaaagttat tatgcgagaa aatgaccttg ctgctcggac gctatgcccg
     3481 gttttccgcg ccgagacggt ggtgggggat cgctggacca tg
//
LOCUS       DATGEM010000700         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_399859, whole genome shotgun sequence.
ACCESSION   DATGEM010000700 DATGEM010000000
VERSION     DATGEM010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399859"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VKQ27_21510"
     CDS             <1..993
                     /locus_tag="VKQ27_21510"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLY91575.1"
                     /translation="FLFFPFIIQWSIGSFVSSSGIALLALLAPVGAMVCFGPRQSIPW
                     FIAYVVLTILSGVFDFFLTTGEGNGMPMKTVAVFFVMNFTIISTMVYLLLRHFVQERD
                     RFESQLAEQHDLVRAEREKSEKLLLSILPGHVAERLKHQPGNIADGFADVSVMFADVV
                     NFTRLAEELTPNQVVSFLDEVFTRFDQLTEQYALDKIKTIGDAYMVVGGLAENRLNYV
                     DHVADMALEMIELTRSDPKLLRLGVQLHIGIATGPAIAGVIGAKRFIYDLWGDTVNLA
                     SRLTSEAEPGTILVDKTTFRRLGHRYSCDLPKDIEIKGKGTTTTYRLSTKLYTV"
     gene            complement(981..3704)
                     /gene="glnE"
                     /locus_tag="VKQ27_21515"
     CDS             complement(981..3704)
                     /gene="glnE"
                     /locus_tag="VKQ27_21515"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HLY91576.1"
                     /translation="MPSAADLPKDFPPALTRAARLSRYVRDLLSADPGLAAGIPLDAP
                     LDRAWMRSWIQGCDNDDQTLKTTLRRLRKAVMLGLITRDLDGRADLAEVVTTVTALAE
                     EAVSAVSASHTLWLAREYGNPLGASSGSRQALQVVAMGKLGGGELNVSSDIDIVFVYP
                     EEGETDGPRRISNHEFFVRLARRIIGAIAEVTADGFVFRVDTRLRPYGDSGPLAVSFD
                     MLEEYFTSQGREWERYAWVKARTVTGAAVAELDAVVTPFVYRRHLDYSAIASLRNLHT
                     QIRQEVHKREIADNIKLGAGGIREIEFLVQVFQIIRGGREPAFRVRSTLKALALLGEK
                     HILPTHAVEELRAAYVYLRNLEHRLQYLDDQQTQMLPPAEEDRALVAEMMGCADYAAL
                     RRELDAHRAVVSRHFEAIFASVRDSGAAQDHSDPWLQSLSEEEARARLAGMGFADPER
                     LHARLVEINGSARYRRMAASSQALLDRLLPQLLDTARALPNPDATFERILNVVDSIGR
                     RESYLALLLEYPNATAALARLASASPWAADYLARHPVLLDELINPQGGSDEPDWPRLR
                     EALRTSLDEHIDNTERQMDILRHFKQTQTLRLLALDLAGTLSLETLSDHLSDLAGAVL
                     EEVLRLAWKALRTRHREQPRFAIIGFGKLGGKELGYASDLDVIFLYDDDDAAAPETYA
                     RLAQRINTWLTSATSAGVLYETDLRLRPDGAAGLLVSHIDAFRDYEFEKAWVWEHQAL
                     TRARFVAGDPEIGARLERVRTEVLCRQRELAALAGEILSMRDRMRDAHPNASGLFDLK
                     HDRGGIVDVEFVVQFLVLGHAHRHPGLTRNVGNLALLKLAAGLGLLPDELATGAHAAY
                     REFRRLQHAMRLQGERFARVDPAVVAQHAKAVQQLWTWVFGDRRQAPAQTV"
     gene            3910..>4961
                     /locus_tag="VKQ27_21520"
     CDS             3910..>4961
                     /locus_tag="VKQ27_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91577.1"
                     /translation="MFLETCFAFLKHGSLWTYRIATYAVLAAGLGFVALVIGLRYLVL
                     PHVNEYREPIAQAISRAVGQQLTIGSVTGSWQGYRPEMTLTDVKVFGADGQPALALER
                     VSTVLSWLSLLSAKWRFDTLAVYGPTLVVKRDASGVLWIAGIAIKPQDGAGGGFGDWL
                     LAQRQVLVRDATITWLDEMRSAPQLQLDKVNVRLDRDGAIHRFGVTAVPPAQLASPLV
                     ARGEFVGHKIRDLQSWNGRLYAEIDYADLALAQTWIPVPLEVSSGFGSLRLWLDLNGT
                     RLGAATADLRLVNVRTRLAADLPDLALSEVYGRLGWTRRGDRTEISATALGFTAAGGL
                     RLAPMHFRYAFSEPAGG"
BASE COUNT          823 a         1573 c         1607 g          958 t
ORIGIN      
        1 ttcctgttct ttcccttcat catccagtgg tcgatcggca gtttcgtgag ttcttccggc
       61 attgcacttc tggcgctgtt ggcgccggtg ggggccatgg tctgcttcgg gccgcgccag
      121 tcaatcccct ggttcatcgc ctatgtggtg ctgacgatcc tgtcgggcgt gttcgacttc
      181 tttcttacca cgggggaagg caacggcatg ccgatgaaga ccgtggcggt gtttttcgtg
      241 atgaatttca cgatcatctc caccatggtc tacctgctgc tgaggcactt cgtacaggag
      301 cgcgaccgct tcgagagcca gctcgcggag cagcacgatc tggtgcgtgc cgagcgcgag
      361 aaatccgaga agctgctgct gagcatcctg ccaggacatg tggccgagcg cctgaagcac
      421 caacccggca acatcgccga cggatttgcc gatgtgtccg tgatgtttgc cgacgtggtg
      481 aatttcaccc ggctggccga ggaactcacc cccaaccagg tggtctcgtt cctcgacgag
      541 gtattcaccc gcttcgacca gctcacggag caatacgcgc tggacaagat caagaccatc
      601 ggtgacgctt acatggtggt cggcggcctt gcggaaaacc ggctgaacta tgtcgatcac
      661 gttgccgaca tggcgctgga gatgatcgag ctgaccaggt ccgacccgaa attgctccgc
      721 ctcggcgtgc agctgcacat cggtatcgcc accgggcccg cgatcgccgg cgtcatcggc
      781 gccaagcgct tcatctacga tctgtggggc gacacggtca atcttgccag ccgtctgacc
      841 tccgaagcgg agcccggcac gattctggtg gacaagacga ccttccgccg tctcggacat
      901 cgctattcct gcgatttacc gaaggacatc gagatcaagg gcaagggcac cacgaccact
      961 tatcgtctgt ccaccaagct ctatacggtc tgagccggcg cttgccggcg gtcgccgaat
     1021 acccatgtcc aaagttgctg caccgccttg gcgtgttgcg ctactacggc aggatcgacg
     1081 cgcgcaaagc gctcgccctg caatcgcatc gcgtgctgca gtcggcggaa ttcgcggtac
     1141 gccgcgtgtg cgccggtagc caattcatcg ggcagcaaac cgagcccggc ggcgagcttc
     1201 aacaaggcca gattgccgac gttgcgcgtc aggccggggt gacggtgggc atggcccagt
     1261 accagaaact gcactacgaa ttcgacatca acgatgccac cgcgatcgtg cttgagatcg
     1321 aacagtccgc ttgcattcgg gtgggcgtca cgcatccggt cgcgcatgga caggatttcg
     1381 cctgccagcg cggcgagttc gcgctggcga cataacactt cagtgcggac gcgctcgagg
     1441 cgagcgccga tttccgggtc gccggcgacg aaacgggcgc gcgtgagcgc ctggtgttcc
     1501 cagacccagg ctttctcgaa ttcgtaatcg cggaacgcgt cgatatggct taccagcagt
     1561 cccgccgctc cgtcgggccg cagcctgagg tcggtttcgt agagcacacc ggccgacgtt
     1621 gcgctggtga gccaggtgtt aatgcgctgg gcgaggcgcg cataggtttc cggggcggcc
     1681 gcatcgtcgt catcgtagag aaagatgacg tcgagatcgg aggcgtagcc cagttccttg
     1741 ccgccgagtt tgccgaagcc gatgatcgcg aaccgcggtt gctcgcggtg gcgcgtgcgc
     1801 aatgccttcc acgccagtcg cagcacttct tccagtaccg cgccggcaag atcgctaagg
     1861 tggtcggaca gggtctccag cgacagcgtg ccggccaggt cgagtgccag caggcgcagc
     1921 gtttgcgtct gcttgaaatg gcgcaggatg tccatctgcc gctcggtgtt gtcgatatgc
     1981 tcgtcgaggc tcgtcctcag cgcttcgcgc aggcgaggcc agtcgggctc gtcgctgccg
     2041 ccctggggat tgatcagttc gtcgagcaaa accgggtggc gagcgaggta gtcggcagcc
     2101 catgggctgg cggaagcgag ccgcgccagg gcggcggtcg cattcggata ttccagcagc
     2161 agcgccaggt aagattcgcg ccggccgatg ctgtccacga cgttcagaat gcgttcgaat
     2221 gtcgcgtccg gattcggcag tgcgcgcgcg gtatcgagca actgcggcag cagccggtct
     2281 agcaacgcct ggctcgaggc cgccatgcgc cggtagcgcg cactgccgtt gatctcgacg
     2341 agccgtgcgt ggagacgttc cggatcggca aagcccatgc cggccagccg cgcacgggct
     2401 tcttcctccg acagtgactg cagccaggga tcggaatggt cctgggccgc gccgctgtcc
     2461 cggacgctgg cgaagatggc ctcgaaatgc cgcgacacca ccgcgcgatg cgcatcgagt
     2521 tcccggcgca gcgcggcgta atcggcgcaa cccatcatct cggcgaccag cgcgcggtcc
     2581 tcctcggcgg gtggcagcat ctgggtctgc tggtcgtcga ggtactgcaa gcggtgttcg
     2641 agattgcgca gatagacata ggccgcgcgc aattcctcga cggcgtgcgt cggcaggatg
     2701 tgcttctccc ctaacagcgc cagcgctttt agcgtggagc gcacgcgaaa cgccggttcg
     2761 cgcccgccgc ggatgatctg gaacacctgc accaggaact cgatttcgcg gatgccgccc
     2821 gcgccgagct tgatgttgtc cgcaatttcg cgcttgtgca cttcctggcg aatctgtgta
     2881 tgcaggttgc gcaggctcgc tatcgcactg taatcgaggt gacgccggta cacgaacggc
     2941 gtcaccaccg cgtcgagctc cgccacggct gcgccggtga ccgtccgtgc ctttacccag
     3001 gcgtagcgct cccattctcg tccctgagaa gtgaaatact cctcgagcat gtcgaaactg
     3061 accgccagcg gaccgctgtc gccgtagggc cgcaagcgtg tatcgacccg gaacacgaag
     3121 ccgtcggcgg tgacttcggc gatggcgccg atgatcctgc gcgcgagccg gacgaagaat
     3181 tcgtggttgg aaatgcgtct cggcccgtcg gtttcgccct cctcggggta gacgaacacg
     3241 atatcgatgt ccgaagacac gttgagttcg ccgccgccga gcttgcccat ggcgacgacc
     3301 tgaagcgcct gcctgctgcc tgaactggcg cccagcgggt tgccatattc ccgcgccagc
     3361 cacagggtgt gactcgctga caccgcgctc acggcttctt cggccagcgc cgttacggtg
     3421 gtgacgacct cggccaggtc tgcacggccg tcgagatcgc gtgtgatcag gcccagcatg
     3481 acggccttgc gcaatcggcg cagcgtggtt ttgagcgtct gatcgtcgtt gtcgcagccc
     3541 tggatccagg aacgcatcca ggcgcgatcg agcggggcat ccagcgggat cccggctgcg
     3601 aggcccgggt ctgccgacaa caggtcgcgc acgtagcgcg acaagcgcgc cgcccgagtc
     3661 agcgccgggg ggaaatcttt cggcaggtcg gcggcagacg gcatgcgtcg ttggattaga
     3721 atgagtgtgc ttcgatcggc gacgttgtca cagccggtgc ggcgccggcc tatgtgcggc
     3781 ccgtgccgca cggcccccgt caggcggctc gtgcagatgc gatgggcgcc cggtgaattt
     3841 tatttacgtc cgcctatctt aaccgaatag cgacagtctc caccgcaccg aaaagcgcgc
     3901 cgtcaatccg tgttcctcga aacctgtttc gctttcctca agcatggttc gctctggacc
     3961 tatcgcatcg cgacctatgc ggttctggct gcgggtctgg gcttcgtcgc gctggtcatc
     4021 ggcctgcgtt acctggtgct gccgcatgtc aacgagtatc gcgagccgat cgcgcaagcg
     4081 atttcgcgcg cggtcggcca gcagctgacg attggttccg ttaccggaag ctggcagggc
     4141 tatcggccgg agatgaccct cacggacgtg aaggtatttg gcgcggacgg ccagccggca
     4201 ctcgcgctcg aacgtgtttc gaccgtcctg tcatggctgt ccctgctgtc cgccaagtgg
     4261 cgcttcgaca cgcttgcggt ttacgggccg actctcgtag tcaagcgcga cgcctccggc
     4321 gtactgtgga tcgccggcat tgcaatcaag ccgcaggacg gtgcaggtgg cggattcggc
     4381 gactggttgc tggcccagcg tcaggtgctt gttcgcgatg ccactatcac gtggctggac
     4441 gaaatgcgca gcgcgccgca acttcagctc gacaaggtca atgtccggct cgaccgcgat
     4501 ggcgcgatcc atcgcttcgg cgtgacagcc gtgccgcctg cgcagcttgc ctccccactt
     4561 gtggcgcgcg gggaattcgt tggacacaag atccgcgacc tgcagtcgtg gaacggaagg
     4621 ctctatgccg aaatcgatta cgccgatctc gcgctcgcgc agacgtggat accggtgccg
     4681 ctggaagttt ccagcggttt cgggtctttg cgcctgtggc ttgatctgaa cggcacgcgt
     4741 ctgggcgcag ctaccgccga tctccggctg gtcaatgtgc ggacgcgact ggccgctgat
     4801 cttcccgacc tcgccttgtc ggaggtgtac ggccgcctgg ggtggacgcg gcgcggcgat
     4861 cgcaccgaaa tttccgcgac tgcactgggc tttacggccg ccggcggcct gcggctggcg
     4921 ccgatgcatt tccgctacgc cttttccgag ccggctggcg g
//
LOCUS       DATGEM010000701         7129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_98032, whole genome shotgun sequence.
ACCESSION   DATGEM010000701 DATGEM010000000
VERSION     DATGEM010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98032"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="VKQ27_21525"
     CDS             complement(<1..1804)
                     /locus_tag="VKQ27_21525"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLY91578.1"
                     /translation="MHFRRLRIAGFKSFAEPVSVEIHPGLTGIVGPNGCGKSNVVEAL
                     RWAMGEQSTRSLRAGEMDDVIFAGTAGRPSRNLAEVTVTLDEALGAAPPPFHEQPELE
                     IVRRIERGSGSIYRVNGKEVRARDVQTLFADLATGAHASGMVSQGRVGALVTAKPEDR
                     RAVLEEAAGITGLHARRHEAELKLRAAEANLSRAEDLCGQLEVQLGGLKRQARQASRY
                     RNISGAIRQAEAELLAIQRATADAARTEAQAALHAARMAVATATEAATGATAHAAEAA
                     AALPALRSEEADARTALERHRVAQEQIAAEEERARAALADATRRMAQLGEDLRHAEQL
                     RHESEAADIRLTAEDAALGQADSDHFARAEAAESMVAEAAEAVRLAEAEANRATEAAA
                     EANARAQTVARQLAEAEQRWRRLEDQFARVNAEREQVAARSIAASALEAAEAEQAAAD
                     AALAAARVELEAAESARAATGPALAAARERRAAADTAQAKLAAEARALAEVLAVKDGE
                     RWPPMVDELAVPAGLETALGAALGEELTSAADRDAARHWRELPAFDPVPALPDGAVSL
                     AGIVQGPAALTRALSQIGLVEGDEAGESRQALLAP"
     gene            complement(1826..2419)
                     /locus_tag="VKQ27_21530"
     CDS             complement(1826..2419)
                     /locus_tag="VKQ27_21530"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLY91579.1"
                     /translation="MPGTRRALLSLVLAGAAVPAAVAAPPDPRETDRAIGGQGAPHGV
                     IECFSLTCPHCADFALEAFPAIKSKWIDPGHLRWIFYDFPTDRAALLAAMVARSLPAD
                     SYVSFIGSLFASQAAWAYGKGSTADALWLRASDAGMDRTSFDKAIGDTHLRDWIVSRA
                     MDAQTRWKVDATPGFVIDGKLYEGAMSADAFSQLLGG"
     gene            complement(2532..3236)
                     /locus_tag="VKQ27_21535"
     CDS             complement(2532..3236)
                     /locus_tag="VKQ27_21535"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HLY91580.1"
                     /translation="MPLSRRSMLSIAGGAVVIGAAGYTGWARYLSPTSTSLAAPPPAT
                     AAPGQLQQSATPASDQKTQHEAERSLGDPSAKVTVAEFFSLTCIHCAAFARETMPQVE
                     KDLIQTGKVRFVYHDFPLDQIALTAAMVARYLPPAQYYPFISALFASQDRWAFARGIN
                     NTDEIWKIAALAGMSQDTFNAAIADADLRNWILMQQKHDEEAFSIDSTPSFVINGKKY
                     AGEMSYDAFHKALPDA"
     gene            complement(3262..3741)
                     /locus_tag="VKQ27_21540"
     CDS             complement(3262..3741)
                     /locus_tag="VKQ27_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HLY91581.1"
                     /translation="MNSEDRHVYGPRPVGTLMQHITRPAFRRRAPATAQVLADWTAIV
                     GPAIAAVTTPRRLSAGTLTIACSGPIAMELQHLAGEVMARINTHLGSQTVTALRFVQT
                     LERTLPPLAASAPPDPAELAAVEATLSGLPDGELRAALAALGRSVAIRPSTPRPPRR"
     gene            3752..4807
                     /locus_tag="VKQ27_21545"
     CDS             3752..4807
                     /locus_tag="VKQ27_21545"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HLY91582.1"
                     /translation="MSLAASALLAWYDRHRRSLPWRAEPGVIAEPYHVWLSEIMLQQT
                     TVAATIPYYERFLIRFPSVHALAAAPLEDVLAAWAGLGYYARARNLHACARAVVAAGG
                     FPHDLAGLRALPGIGDYTAAAIGAIAFGVPAVPVDGNVERVVARLFSVTEPMPRAKPI
                     LRANAAVLGADKAAQARPSDFAQALFDLGATICTPAAPACALCPWMADCAGRLAGIAR
                     ELPRKAEKRVRPLRHGVHFWLADGAGNVLLRRRPPEGLLGGMTELPGTEWRTEPWQAA
                     DALLHAPMCADWAPAGQVRHGFTHFELAIDLFAAQVPRIDAHGFVRPVAALANEALPS
                     VMRKCVRMAGAVGSFHR"
     gene            4887..5636
                     /locus_tag="VKQ27_21550"
     CDS             4887..5636
                     /locus_tag="VKQ27_21550"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HLY91583.1"
                     /translation="MDRIRRWLGSLSIKTKMLMGFACVLVILLAVAGTGYWRFQGVAD
                     SLRSYVQSVVVVAASQNIDREFLQMRRHVREFAFTGNPDAATAAMASADRMKVAIEKG
                     LAVATSPERQRYMQDIAPPYEAYRKGFDAVLAMKREKDKLVSYVVDPAGTAAREDLDR
                     LIAGGAKEALPEFSSLARDVQQALMLLRLNGTKVLDRQRDETAAKKAQSAEAELTRIT
                     PLLHAAAVGPEVRAARDVLRQHLNVSGTWCG"
     gene            5633..6037
                     /locus_tag="VKQ27_21555"
     CDS             5633..6037
                     /locus_tag="VKQ27_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLY91584.1"
                     /translation="MISDIAGQTNLLALNATIEAARAGDAGKGFAVVAGEVKALAAQT
                     AKATEEIAAQIAAIRGATSEAVNAVREVSTSISQVSEVAAAIAAAIEQQTATTREIAH
                     NAQTVMTVTQEATHAMQDSPRYRKPPEPPART"
     gene            6034..6447
                     /locus_tag="VKQ27_21560"
     CDS             6034..6447
                     /locus_tag="VKQ27_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLY91585.1"
                     /translation="MKRNAYEVGKTAGVLRSELSMFLEAVAKSDEDDRRRYERIPGMG
                     AIATLHAPGRGATHVVINDISRGGVGQRCDWQAAGGAEVEIDLPGSDKAVVARMVRRR
                     DGLLALAFRQDAAVLRRVDLALDHISRQTVARAAA"
     gene            6532..>7129
                     /locus_tag="VKQ27_21565"
     CDS             6532..>7129
                     /locus_tag="VKQ27_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HLY91586.1"
                     /translation="MQVRAAIARKAGDRLSLETVELDGPRDGEVLVEIKATGICHTDE
                     YTLSGADPEGIFPAILGHEGAGVVVDVGRGVTSLKKGDHVIPLYTPECRQCEYCLSRK
                     SNLCVAIRATQGKGVMPDGTSRFRAGSETVYHYMGTSTFANYTVLPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVMNTAKVEAGARCIVFGLGGIG"
BASE COUNT         1166 a         2403 c         2408 g         1152 t
ORIGIN      
        1 cgggcgcaag aagagcctgg cgcgattcgc ctgcttcatc gccctcgacc aggccgatct
       61 gtgacagcgc gcgggtcagc gcggcaggcc cctggacgat gcccgccagc gagaccgccc
      121 cgtccggcag tgccggcacc ggatcaaacg ccggcagttc gcgccaatgc cgggcagcgt
      181 cgcggtcggc agcggaggtc agctcttcgc ccagcgcggc gccaagcgca gtctccagac
      241 cagccggcac ggcgagttcg tccaccatcg gcggccagcg ttcgccgtcc ttcaccgcca
      301 gaacctcggc cagcgcgcgc gcctcggcgg cgagcttggc ctgagccgtg tcggccgcgg
      361 cgcgccgctc gcgggcggcg gccaatgcgg gacccgtggc ggcacgggcg ctctccgcgg
      421 cctccagctc aacacgcgca gcggcgagcg cagcatcggc ggctgcctgc tcggcttccg
      481 ctgcctcaag cgcggaagct gcaatcgagc gcgcggcgac ctgctcgcgc tcggcgttca
      541 cgcgagcgaa ctggtcttcc agacggcgcc agcgctgctc cgcctcggca agctggcggg
      601 caacggtctg ggcacgtgcg ttggcttcgg cggcggcctc agtggcgcga ttcgcctcgg
      661 cttccgccag ccggacagct tccgcagcct cagccaccat cgattcggct gcctcggccc
      721 gtgcgaaatg atccgaatcc gcctgcccca gcgccgcatc ttcagcagtg aggcggatgt
      781 ccgccgcttc cgattcgtgg cgaagttgct cggcgtgccg caggtcctcg ccaagctgcg
      841 ccatccgcct ggtcgcgtcc gccagcgcgg cccgcgcgcg ctcttcctca gcagcaatct
      901 gctcctgcgc cacgcgatgg cgttcgaggg cggtacgcgc gtccgcctcc tcgcttctca
      961 gcgcagggag cgccgcggcg gcctcggcag catgggcggt ggccccggtt gcggcttcgg
     1021 tcgctgtcgc aaccgccatc cgcgccgcat gcagcgccgc ctgcgcctcg gtccgggccg
     1081 catcggccgt ggcgcgctgg atggccagaa gttccgcctc cgcctggcgg atcgccccgg
     1141 atatgttgcg gtagcggctt gcctgccgcg cctggcgctt cagcccgcca agctgcacct
     1201 ccaattggcc acacaggtcc tcggcgcgcg acaggttcgc ttctgcggca cgcagcttca
     1261 gttccgcctc gtggcgacgg gcgtgcaggc cggtgatgcc ggccgcttct tccagcacgg
     1321 cgcgcctgtc ctcgggcttc gccgtcacca gcgcaccgac ccgcccctga ctcaccatgc
     1381 ccgacgcgtg cgccccggtc gccaggtccg cgaacagcgt ctgcacatcg cgcgcccgca
     1441 cttccttgcc gttcacccgg tagatgctgc cggagccccg ctcgatgcgg cgcacgatct
     1501 cgagctccgg ctgctcgtgg aacggcggcg gcgcggcgcc cagggcctca tccagcgtca
     1561 ccgtcacctc ggccagattg cgcgaaggcc ggccggcggt gccggcgaag atcacgtcgt
     1621 ccatctcgcc ggcgcgcagc gagcgcgtgc tctgctcacc catggcccag cgcagcgcct
     1681 ccaccacgtt ggatttgcca cagccgttcg ggccaacgat tccagtcagc ccaggatgga
     1741 tctccacgct gaccggttcg gcaaaactct tgaatccggc gatccgcagg cgacgaaaat
     1801 gcactgacaa gcgcgaactc ctccgctagc cgccgagcaa ctgcgagaac gcgtcggcgg
     1861 acatggcgcc ctcgtagagc ttcccgtcga tcacgaagcc cggagtcgca tcaactttcc
     1921 aacgtgtctg ggcatccatc gcacggctca caatccagtc acgcagatgg gtgtcgccga
     1981 tcgccttgtc gaagctcgtg cggtccatgc cggcgtcgct ggctcgcagc caaagcgcgt
     2041 cggcggtact gcccttgccg taggcccagg ccgcctggct ggcgaacagg ctgccgatga
     2101 aggacacgta gctgtccgcg gggagcgagc gggcaaccat ggcggccagc agcgccgcac
     2161 ggtcggtagg gaaatcgtag aaaatccagc gcaggtggcc gggatcgatc cacttcgact
     2221 tgatcgcggg aaacgcctcc agggcgaagt ccgcgcaatg tgggcaggtg agcgagaagc
     2281 actcgatcac gccatgcggc gcgccctgac cgccgatggc gcgatcggtc tcgcgcggat
     2341 ccggcggcgc ggcgacagcg gcaggaaccg ccgctccggc gagaaccaag gatagcaggg
     2401 cgcgccgggt gcctggcatg tttcccccag atcctgtgcg agagcacagg ccgcgacaag
     2461 tcagcaagag ccaggagcgc gggcggtgat ttctctgccg ctccgcccct cgcaatggcg
     2521 gccttcggcg actaggcgtc cggcagcgcc ttatggaagg cgtcgtagct catctcgccc
     2581 gcgtatttct tgccgttgat cacgaaactg ggagtcgagt cgatgctgaa cgcttcctcg
     2641 tcgtgctttt gctgcatgag gatccagttc ctgaggtccg catcggcgat cgccgcattg
     2701 aaggtgtcct ggctcatgcc ggcgagggca gcaatcttcc agatctcgtc ggtattgttg
     2761 atgccgcgcg caaaggccca gcggtcctgg ctggcgaaca gcgcgctgat gaaggggtag
     2821 tactgcgcgg gcggcagata gcgggccacc atcgctgccg tcagcgcgat ctgatccagc
     2881 ggaaagtcgt gatagacgaa gcgcaccttg ccggtctgga tcaggtcctt ctcgacctgc
     2941 ggcatcgtct cgcgggcaaa agcggcacag tgaatgcaag tgagcgagaa gaattcggca
     3001 accgtgacct tggccgacgg atcgccaagg ctccgctcag cctcatgttg cgtcttctga
     3061 tccgatgccg gcgtcgccga ttgctgcagc tgccccggcg ccgcagtcgc aggtggcggc
     3121 gcggcgagcg aggttgacgt cgggctgaga tacctggccc aaccagtata gcccgcggcg
     3181 ccgataacga cggcaccccc agcaatcgac aacatggaac ggcgagagag tggcattggg
     3241 cacctctgtt ggtcggcggg attagcgtct cggcgggcgt ggtgtcgagg gacggatagc
     3301 cacggatcgg cccagcgccg caagtgctgc gcgcagctcg ccgtcgggca ggccggatag
     3361 cgtcgcctcc accgcggcaa gctccgccgg gtccggcggc gccgatgcag ccaatggcgg
     3421 cagcgtgcgc tcgagcgtct gcacgaagcg cagcgcggta acagtctgac tgccgagatg
     3481 ggtgttgatc cgggccatga cctcgccggc cagatgttgc agttccatgg caatcggccc
     3541 ggagcaggcg atggtaagcg tgccggcgga gagccggcgc ggcgtggtca ccgcggcaat
     3601 cgccggtccg acgatggcgg tccagtcagc gagcacctgc gcggttgcgg gcgcccggcg
     3661 acgaaaggcg ggacgcgtga tgtgctgcat cagcgtgccc accggccgcg ggccatagac
     3721 gtgacggtct tcgctgttca taccgctacc cgtgtcgctc gccgcttctg ccctgctcgc
     3781 ctggtatgac cggcatcggc gctcactgcc gtggcgcgcc gaaccgggtg ttatcgccga
     3841 accttaccac gtctggctca gcgagatcat gctgcaacag accacggttg cggcgacgat
     3901 cccgtactat gaaaggttct taatccggtt cccctccgtc cacgcccttg ctgccgcgcc
     3961 attggaggac gtgctcgctg cctgggcagg cctcggatac tatgcacgcg cgcgcaacct
     4021 ccacgcctgc gcccgcgctg tggtcgccgc cggcgggttc ccgcacgacc tcgccgggct
     4081 gcgcgccttg ccaggaattg gcgattacac cgccgccgcg attggcgcga ttgccttcgg
     4141 tgtgcccgct gtgccggtgg atggcaacgt cgaacgggtt gtcgctcgcc tgttctccgt
     4201 aacggagccc atgccacgcg cgaagcccat actgcgcgcg aatgcggcgg tgctcggcgc
     4261 cgacaaggca gcacaggcgc ggccgtcgga tttcgcgcag gcactgttcg atcttggtgc
     4321 aacgatatgc acgccggcgg cgcccgcctg tgcgctttgt ccctggatgg cggattgcgc
     4381 cgggcgactg gccggcatcg cccgtgagct gccgcgcaaa gcggaaaagc gcgtccggcc
     4441 gctgcgtcac ggtgtgcatt tctggcttgc cgacggcgct ggcaacgtgc tcctgcggcg
     4501 acggccgccg gagggcctgc tgggcggaat gaccgaactg ccgggcaccg aatggcgcac
     4561 cgaaccgtgg caggcagcgg acgcattgct gcatgcgccg atgtgcgccg actgggcgcc
     4621 cgccgggcag gtacggcacg gcttcaccca tttcgagctg gcgatagacc tgttcgcagc
     4681 acaggtccct cgcatcgatg cccacggctt tgtccgcccg gtggcggcac tggccaacga
     4741 ggcgctgccg tcggtgatgc gcaaatgcgt gcgcatggcc ggcgcggtcg gttcgtttca
     4801 ccgataatta agcctcatcg ccgaacctcc aacttcccga ggggccgtgc acctcgcggc
     4861 tctggctcag gtttggagct tcacccatgg accgcattcg tcgctggctt ggttcgcttt
     4921 cgatcaagac gaagatgctc atgggtttcg cctgtgtgct ggtcatcttg ctggctgttg
     4981 caggcaccgg gtactggcgc tttcagggcg ttgccgattc cttgcgctcc tatgtccaga
     5041 gcgttgtcgt cgtggcagcg agccagaaca tcgatcgcga attcctccag atgcgccggc
     5101 acgtgcgcga gttcgcattc accggcaatc cggatgcagc aaccgcggcc atggcatcgg
     5161 cagatcgcat gaaggttgcg atcgagaagg gcctggcagt cgcgaccagc cctgaacgcc
     5221 agcgatatat gcaggacata gcgcccccgt acgaggctta tcgcaaaggc tttgatgccg
     5281 tcctcgcgat gaagcgcgag aaggacaagc ttgtcagcta cgtagtcgat ccagcgggca
     5341 cggcggcgcg agaagatctc gatcggctga tcgccggcgg cgccaaagag gcgttgccgg
     5401 aattctcgtc attggcgcgt gacgtccagc aggcgctgat gctgctccgg ttgaatggta
     5461 ccaaggtcct cgatcgtcag cgcgacgaaa cggcggcgaa gaaggcgcaa agtgccgagg
     5521 ctgagctcac gcgcattaca ccgctgctgc acgccgcggc tgtgggcccc gaggtccgtg
     5581 ccgcgcgcga tgtgctgcgg cagcatctga acgtgtcggg gacgtggtgc ggttgatctc
     5641 cgatattgcc ggccagacca acctgttggc gttgaatgcg acaatcgagg cggcgcgggc
     5701 cggcgatgcg ggcaaaggct tcgccgtggt cgctggcgaa gtgaaggcac tcgccgcgca
     5761 gaccgccaag gcaaccgagg aaatcgccgc gcagatcgcc gctatccgcg gcgcgacaag
     5821 cgaggcggtc aacgcggtcc gcgaggtcag tacctcgatc agccaggtaa gcgaggttgc
     5881 cgcggcgatt gccgcagcga tcgagcagca gaccgcgaca acacgggaaa tcgcccacaa
     5941 cgcgcaaaca gtgatgactg tcacgcaaga ggctacacac gcgatgcagg attctccgcg
     6001 atatcggaag ccaccggagc cgccagcgag gacgtgaaac ggaacgccta cgaagtgggc
     6061 aagaccgcgg gcgtgctgcg gtcggaattg tccatgttcc tcgaggcggt ggcaaaatcc
     6121 gacgaagacg atcggcggcg ctacgagcgg atccctggca tgggtgcgat agctacgctg
     6181 catgcgccgg gtcgtggagc aacgcatgtt gtgatcaacg atatctcgcg cggcggcgta
     6241 gggcagcgat gcgattggca ggcggccggc ggcgcggaag tcgagatcga cctgccgggt
     6301 agcgacaagg ccgttgtcgc ccgaatggtg cgcaggcgcg acggcctgct ggccctggcc
     6361 ttccgccagg atgccgcagt cctgcggcgc gtggatctgg cactggatca tatcagccgc
     6421 cagacagtcg ccagggcagc agcataaacc gccccccgcg cgacgcctcg ccgcttgatc
     6481 gcaggcggcg ctgcccgcac catcccggca tgcaaggccg ggagagtgac aatgcaggtg
     6541 cgcgccgcaa tagcccgcaa ggcaggagac aggctgagcc tcgagaccgt cgaattggac
     6601 ggtccgcgtg atggcgaggt gctggtggag atcaaggcca ccggcatctg ccataccgat
     6661 gaatacacgc tgtccggcgc cgatcccgaa gggatatttc cggcgatcct gggccatgag
     6721 ggcgccggcg tcgtggtgga tgtcggccgc ggcgtgacca gcctgaagaa aggcgaccac
     6781 gtcattccgc tctacacccc ggaatgccgg cagtgcgaat actgcctctc ccgcaagagc
     6841 aacctgtgcg tcgcgatccg cgccacccag ggcaagggcg tgatgccgga cggcaccagc
     6901 cggttccgcg ccggcagcga gacggtgtac cattacatgg gcacctcgac cttcgccaac
     6961 tacacggtat tgcccgagat cgcgctcgcg aagatccgtg aggacgcgcc attcgacaag
     7021 gtctgctaca tcggttgcgg tgtcaccacc ggcatcggcg cggtgatgaa caccgcgaag
     7081 gtggaagccg gcgcacgttg catcgtattc ggcctcggcg gcatcggcc
//
LOCUS       DATGEM010000702        15174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_171498, whole genome shotgun sequence.
ACCESSION   DATGEM010000702 DATGEM010000000
VERSION     DATGEM010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 15174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15174
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171498"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..596)
                     /locus_tag="VKQ27_21570"
     CDS             complement(153..596)
                     /locus_tag="VKQ27_21570"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HLY91587.1"
                     /translation="MDHAIDIACREELRRAGLPGQGVLVRLLALLRSAPEPHLSLGQV
                     SRIMAENNLATPSGELDRQLRILADHGLLGRLPDTAAEPVFDTEPEPHSHLVYEETNQ
                     IVDLHVSPETLLAVIRQALAEQSDRVEIIVRVRGPSRGERAISDR"
     gene            complement(724..2910)
                     /gene="katG"
                     /locus_tag="VKQ27_21575"
                     /pseudo
     CDS             complement(724..2910)
                     /gene="katG"
                     /locus_tag="VKQ27_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
     gene            3179..4084
                     /locus_tag="VKQ27_21580"
     CDS             3179..4084
                     /locus_tag="VKQ27_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLY91588.1"
                     /translation="MNLRDLRYLLAVAEYEHFGRAAEACEVSQPTLSVQIRRLEELLG
                     VALFERTSRAVVPTPACDQLIGHVRAAVAEMDAVFAVASSMRDPLAGRIRLGIIPTLA
                     PYLLPLVFAPLRDALPMLDVEPWEDQTAALLKRLHAHELDAAVLATEVSGPDLESREL
                     FAEPFLAALPPEHPLARREVVAEADLAKDILVLADGHCLRDQALEVCGGTGALNGTLR
                     ATSLSTLLNMVAAGYGTTLIPALAAGAAQDTGIVLRPLAARVGRTVRIAWRTRFPRRA
                     ALERVGEVIAARLQGFAEGAEAGTI"
     gene            complement(4389..5006)
                     /locus_tag="VKQ27_21585"
     CDS             complement(4389..5006)
                     /locus_tag="VKQ27_21585"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HLY91589.1"
                     /translation="MALAAACLPGTQAAANLLVNGSFEDATNFLDNTGQDTMNVDLGS
                     TAMTGWTVTGSHFVSWIGPGNPFLLTASNGGYFVDLTGYLHGAPFGGVSQTISTTAGA
                     TYQLRFDLGSSSIYGLPSGITASAGSSSQTFTSADTGANNWETETFDFVAGGPTTTIS
                     FLGDQGLEYTGLDNVSVALAMPEPVSFGVLAAGLAALGTARYRRR"
     gene            5939..8008
                     /locus_tag="VKQ27_21590"
     CDS             5939..8008
                     /locus_tag="VKQ27_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91590.1"
                     /translation="MSDLSAASPASSNDGTDNVYRIALSMSGAISAGAYTAGVLDFLM
                     LALAELEREKAARPARFVPPNHDVRIVAMAGASAGGICGPLSTLALGHGMVFPPVEME
                     VDGKKDTGEKQRIFCVLPSLYAAWVEKPRMVARNGVDPSLLTTEDLDNGKVVSALNAG
                     VLNDIATAALRPPAHPATIQAPHEFLAKPLHLYLAISNLPGIQYEVHGSSDKDVYRMV
                     NHADRVHICIRDIGSDRRVASKWAGTDNPSETLDLASLQQDPMKWKALAKASLATAAF
                     PIGLSARALIAKQEIYAGRWFPAPRFGTAAIGPTWPQWEPPMPEPFPFVNVDGGMINN
                     DPFEFARYSLIDDWETRGAANPSAAEDADRAVIMIAPFPEGAVVPTGGYNDDTGLLSV
                     ITRLLPTLTQQVRFKVSELAAAADPTYASRWLIAPRRRDSPDEVPSSDNIACGLLGGF
                     GGFLDQRFREHDFLLGQRNCQNFLRNWTESKFNKTSPNDPWVVRLCGLALSKEIPAPD
                     WPRMEIEALSEVSRRIKIRAKKLVPALVTQEVGSHFVRWLFRVVWWWVRDKVLNMING
                     MMLADLLRRDQIEDGMADGSRDRLRNFTPQERGVFAALALADYDARTVTGLATSLKLT
                     ESQIDGIIQRARALDPAAVHHVVQSRYNSKDGQPSYTLARRRLSAWKKFSAAMPWLNR
                     VAGVKIG"
     gene            8173..8787
                     /gene="ruvA"
                     /locus_tag="VKQ27_21595"
     CDS             8173..8787
                     /gene="ruvA"
                     /locus_tag="VKQ27_21595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLY91591.1"
                     /translation="MIAQLTGRVEALEDGRCVIDVNGVGYLVHASTRTLAALPAPPEG
                     ARLLVETHVREDAIVLYGFIDPAEREWFRLLTTVQGVGGRVALSVLSALSPADLVAAI
                     ASGDRASLTRAPGVGARLAVRLLTELRDKAGAMPTAPGIAAVPVVASRGPGEDAMSAL
                     VNLGYRRMEVQPVVARVIERLGEVATVDAVLRESLRELAQRVGG"
     gene            8784..9854
                     /gene="ruvB"
                     /locus_tag="VKQ27_21600"
     CDS             8784..9854
                     /gene="ruvB"
                     /locus_tag="VKQ27_21600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HLY91592.1"
                     /translation="MSGRRPQPSSPAIGGEDRAVSAERREDDAAEVSLRPQSLEEFVG
                     QQASRENLAVFIAAARARGEALDHVLLHGPPGLGKTTLAQIVARELGVGFRATSGPVI
                     QRAGDLAAILTNLQPRDVLFIDEIHRLQPAIEEVLYPAMEDFQLDLIIGEGPAARSVR
                     IDLAAFTLVGATTRAGLLATPLRDRFGIPLRLVFYTPQELELIVRRGAAKLGVPLTQD
                     GAAEIARRSRGTPRIAGRLLRRVRDFASVAGVEEIDRAMADNALNRLEVDALGLDAMD
                     RRYLRRIGEHHGGGPVGVETLAAALAEARDTLEDVIEPFLIQEGLVLRTSRGRMLGQS
                     GWRHLGLVPPARQPDQFDLLPE"
     gene            9851..10315
                     /gene="ybgC"
                     /locus_tag="VKQ27_21605"
     CDS             9851..10315
                     /gene="ybgC"
                     /locus_tag="VKQ27_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HLY91593.1"
                     /translation="MNGHAVSIPADGQHRYTLRVYYEDTDAGGVVYHATYLRFAERAR
                     TEALRAAGVPHAELLADHGVMFMVRRIEVDYLRPARLDDSLTVVTEPLAVGGASATLR
                     QDVLGPDGPCAILTVRLACVKPGDGRPGRLPQRWRTALTAMCNAATAARNEE"
     gene            10321..11037
                     /gene="tolQ"
                     /locus_tag="VKQ27_21610"
     CDS             10321..11037
                     /gene="tolQ"
                     /locus_tag="VKQ27_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HLY91594.1"
                     /translation="MDRAVDAANLAVPGADLSLWGLFIEADIVVKIVMLGLLAASVWV
                     WAVVFEKWSSLRKVNREADGFEDRFWSGGSLEELFDHEGTNPVHPMAAVFGAAMGEWR
                     RSVRVAGADISRTSVRERVDRAITVTVQREMDRMERWMIFLASVGATAPFIGLFGTVW
                     GIMHSFSAIAAMHNTNLAVVAPGIAEALFATAIGLVAAIPAVLAYNKISNDLTRFASR
                     LEGFGAEFSAILSRQSEERA"
     gene            11041..11541
                     /gene="tolR"
                     /locus_tag="VKQ27_21615"
     CDS             11041..11541
                     /gene="tolR"
                     /locus_tag="VKQ27_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HLY91595.1"
                     /translation="MALTFNTRGNGRGRYRPLSEINVTPLVDVMLVLLIIFMVTAPLM
                     TSGVSVDLPKTDAKPINTDSQPLTVSLDAQGKIFLQDQEVQLPELVTKLQAIAQNNPD
                     RRIFVRGDKDLAYGRIMEVMGTITQGGFTKVALLAEQPTVTPSTPPAPTAPAPVPAPS
                     GQTKSR"
     gene            11552..12583
                     /locus_tag="VKQ27_21620"
     CDS             11552..12583
                     /locus_tag="VKQ27_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91596.1"
                     /translation="MLQRVLRGTDRLDRRNEGVSPTLRVGALISGGVHLAILLLLLIG
                     IPFSRPPAEPPEETSVSMVFGTADSAMKAPAPAAVPAPANTQTTNPAPKATEPPKPQP
                     IEAPPPPPPPPPPPPTPPQSTAPPLPTPPTPVPPPTPAPAAETAPPPPAAPPTPQQAQ
                     PPLPTPPLPVPPPPVPPPPSPQSATSQPNVTKNPAPESDALENTLSKLRSLQKQTQPP
                     KARYNPQSGGAPNGGGNPLSDDTSALSMAQRGAIGDFVRRCWSTDPGMLDLDRMQVLL
                     TVTTDPSGVVRRAVVADDDAGRVGSDMRLRVFAERAIRAVLDPNCSNLPLPQNMLGQT
                     RVFTFRFRP"
     gene            12699..14033
                     /gene="tolB"
                     /locus_tag="VKQ27_21625"
     CDS             12699..14033
                     /gene="tolB"
                     /locus_tag="VKQ27_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HLY91597.1"
                     /translation="MVRAQGVAPPRAPAPGAQGGGQTAVIDVNRARTDPIPIALPALV
                     GSDGASAQLGQDIMGVVSSDLGNSGLFRPVNPSAFIQASAGPAADMPNFANWKAIGAQ
                     ALVTGRAESQGGGQIRVEFRLWDVLPGTQLQGTAYTTTPANWRRIAHIIADVIYERLL
                     GEKGYFDTRICYIAATGPRDRRTKRLAIMDQDSANNRFLTDGSWLVLTPRFHPTRDEI
                     AFMSYANNRPRVYIFNLGSGQQRVLGDFPGMTFAPRFSPDGNSVIMSVVNGGGSDIVS
                     VDLGSHASRRLTNSGAIDVSPCYSPDGSQIVFNSDRGGDQQLYIMSAGGGGAKRISYG
                     QSRYATPVWSPRGDLIAFTRFGGEVGNFAIGVMHPDGSGERILSESFSVEGPTFAPNG
                     RVLMFWRQSAARDSRGSGFSSRLVSIDITGFNERVVETPTDASDPAWSPLAA"
     gene            14201..14668
                     /gene="pal"
                     /locus_tag="VKQ27_21630"
     CDS             14201..14668
                     /gene="pal"
                     /locus_tag="VKQ27_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HLY91598.1"
                     /translation="MNSKILGTLAVVALLAACSDQKTTASTGAGATTSGPVPGSEEDL
                     VANVGDRVFFAFNASNLAPDAQATLDKQAGWLGKYQQVNVQVAGNCDDRGTEEYNLAL
                     GQRRANSSRDYLVARGVAAARITTISYGKDRPTAMGDNEQAWAQNRNTITAVR"
     gene            14796..>15174
                     /locus_tag="VKQ27_21635"
     CDS             14796..>15174
                     /locus_tag="VKQ27_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLY91599.1"
                     /translation="MSIPSALRLILAAIVLVYVTPAWAQVESREGIALQNQILELQRQ
                     VQGLQAQGARGPTNLGGSYSSPPSTSSGDLVAQLLSRVSTLEDQVRQLRGRVDELQNQ
                     QDRQSADLSKKMDDLAFQLQGSAG"
BASE COUNT         2652 a         4579 c         5224 g         2719 t
ORIGIN      
        1 cggctggatg ggattggcag gaatgtgctg ctgataaagc aacacacaat tctgtctgtt
       61 gaggtggatg tcccgtttga tttgaaagtt gagagcgacg acgtacacgt gacattgggt
      121 ttgaacctga agggacagcc gccattctga gtctatctgt cggaaatcgc ccgttcaccg
      181 cgcgatggac cgcggacgcg cacaatgatc tccaccctgt ctgactgctc ggccagcgcc
      241 tggcggatca ccgcaagcag agtttcgggt gagacgtgga gatccacgat ctgattcgtc
      301 tcttcataga ccagatggga atgcggctcg ggttcggtat caaagaccgg ttcggccgcg
      361 gtgtcaggca gtctgccaag cagaccgtgg tcggcgagga tgcggagttg tctgtcgagc
      421 tcgcctgacg gtgtggccag gttgttttcg gccataatgc gcgacacctg cccgaggctc
      481 aggtgtggtt ccggcgcgct gcgcagaaga gcgagcaacc gcacgagtac cccctggccg
      541 ggcaagccgg cacgccgtaa ttcctcccgg cacgcaatgt cgattgcgtg atccatagcc
      601 gttgcctccc gccaaggtgg cgcgaccggg ccgcctcaga gcgattgggt gggccgtctt
      661 gaatgcggcc ttgcttggcg cccggtggaa actgaatgac agctcccacc gggcgcctga
      721 ttgtcaggcg taggcgaggt cgaagcggtc caggttcatg accttgttcc aggccgccac
      781 gaagtcctgc acgaacttcc ctgctgaatc actctggccg tagacctccg cgaacgcgcg
      841 aagctgcgag tgcgaaccga agatcaggtc tacacgtgtg ccagtccatc tgggctgctt
      901 tgtcttcctg tcgtgcccct cgtatgcgcc gttggagccg gagggctgcc actctgtccg
      961 catgtcgagg aggttgacga agaagtcgtt cgtgagcgtt tccggccgac tggtgaatac
     1021 accgtgcttg gagccaccgg cattcgcgcc cagaacacgc aggccgccga cgaggaccgt
     1081 catctcgggt gcagtcaggg tcagcaactg cgccttgtcc accaacgcct cttccggcgc
     1141 catcagctcc ttgccgctca ggtaattgcg gaagccgtca gccgccggtt ccagcacagc
     1201 gaaggattcg acgtcggtct ggtcctgcta tgcatccgtg cggcctggtg agaagggaac
     1261 agtcacgttg tgcccggctg ccttcgcggc cttctccacc gccgcgcatc cgcccagaac
     1321 gatcaggtcg gccagcgaga ctttcttgcc gccggcagcg gagccattga actcctgctg
     1381 gatcgcttcg agtttctgca acacggtcgc gaggtgctgc ggctgattga cggcccagtc
     1441 cttctgcggc gccagtcgaa cgcgcgcgcc gttggcgccg ccacgcttgt cggagccgcg
     1501 gaacgtcgag gccgacgccc aggccgctga gacgaggtgc gacaccgaaa gaccggaccc
     1561 gagaatcttc gccttcagcg cggcgatgtc ctgctcgttg atcagcggat gatcgacttg
     1621 gggaaccggg tcctgccaga tcagtgtctc tttcgggacg agcgggccga ggtagcggat
     1681 acgcggtccc atatcgcgat gcgtgagctt gaaccacgcc cgggcgaacg catcggcaaa
     1741 ctgctccggg tgttcataga agcggcggga gatcttctcg taggcgggat ccatccgcag
     1801 cgagagatcc gtggtcagca tcgtcggtaa gtgcttcttg gacttgtcga aagcgtcggg
     1861 gatcgtgggt tccgcgttct tcgccttcca ttgataggcg ccagccgggc tcttggtcag
     1921 ctcccattcg tatttgaaca ggttgtcgaa gaagtggttg ctccacttcg tgggtgtctg
     1981 cgaccaagtg acctcagggc cgccggtgat ggcgtccgcg ccgtagcccg tgccgtgttt
     2041 gcttttccag ccgaggcctt gatcctcgat gccggcgcct tccggttccg ggccaacgag
     2101 tgacggatcg ccggcaccgt gcgctttgcc gaaggtgtgg ccaccagcaa tcagtgccac
     2161 ggtctcttcg tcattcatcg ccatgcggta gaaggtttcg cggatgtcct tggccgctgc
     2221 gatcggatcc ggcttgccgt tcggtccttc cggattgacg tagatcaggc ccatctgaac
     2281 ggcgccgagg ggattttgca gctggcgttc gccgctgtag cgctcgtcgc cgagccaggt
     2341 gccttcggga ccccagtaga gctcttccgg ctcccacacg tccttgcggc cgccgccgaa
     2401 gccgaacgtc ttgaagccca tggattccag ggcgacgttg ccggccagga tcatcagatc
     2461 ggcccaggag agcttctggc cgtatttctg cttgatcggc cagagcagcc ggcgcgcctt
     2521 gtcgaggttg gcgttgtcgg gccagctgcc gagtggcgcg aagcgctgct ggccggagcc
     2581 agcgccgccg cggccgtcgg cgatgcggta agtgccggcc gagtgccatg ccatgcggat
     2641 catcagaccg ccgtagtgac cgaaatcggc cggccaccag tcctgcgact ccgtcatcag
     2701 cgcctgcagg tccttgatca ctgcgttcag gtccagggtc ttgaacgcct tggtgtagtc
     2761 gaatgcattg cccatcggat tggacagcgc ggaatgctga tggaggaccg tcaggttcaa
     2821 ctggttcggc caccagtccc gattggtccg ccagcccgca tggatcacgg ggcacgtgcc
     2881 cgcggcgacg gcgtcagtct ttgcatccat aagtttctct ccgcttggtc agaaatacgc
     2941 ccgtgccggc agatcgcagg gacacccagg cacggcgcgg ggtcaatctc agcgcgatga
     3001 acatgggccc ggcacaagca ggtcccaacc gcgcaagttg gcagcagatc tgttccggtt
     3061 gccggaggct ccggaaactt cacttgaact gggttagcgg attcccgatg atagatgcaa
     3121 gctgaccaat ccaatagatg cgataggcaa ggtctatcat agcagtgata ctgaccaaat
     3181 gaatctgcgg gaccttcgat atctgcttgc agttgccgag tacgagcatt tcggccgcgc
     3241 tgccgaagcg tgcgaggtca gccagccaac gctgtccgta cagatacgca ggctcgagga
     3301 gctgctggga gtggcgctgt ttgagcgcac gagcagggcg gtggtgccga cacccgcttg
     3361 cgatcaattg atcgggcatg tgcgcgccgc ggtggcggag atggatgcgg tgttcgcggt
     3421 tgcgagcagc atgcgtgatc cgctggctgg gcgtatccga cttggcatca tcccgacatt
     3481 ggcgccgtac ctgctgccgc tggtgttcgc gcctttgcgc gatgcgctcc ccatgctcga
     3541 cgtggagcca tgggaggacc agacggccgc gctgctcaag cggttgcacg cacatgagct
     3601 ggacgctgcc gtgttggcga ccgaggtgag cgggccggac ctggaaagcc gggagctgtt
     3661 tgccgaaccg ttcctcgcgg cgctgccacc cgagcatccg ctcgccaggc gtgaggtggt
     3721 ggcggaggcg gacctggcca aggacatcct ggtgctcgcg gacgggcatt gcctgcgtga
     3781 ccaggcgctg gaggtgtgtg gcgggacggg cgcgctgaac ggcacgctgc gtgcgacaag
     3841 cctatcgacc ctgctgaaca tggtggccgc ggggtacggg acgacgctga tacccgcgct
     3901 cgcggcggga gcggcgcagg atacggggat cgtgctgcgg ccgcttgccg cccgggtagg
     3961 gcggacggtg cggatcgcgt ggcgcacccg cttcccccga cgcgctgcgt tggagagagt
     4021 gggcgaggtg attgccgcac gcctgcaggg gttcgcagaa ggtgcggagg cggggacgat
     4081 ctaggatccg gggggttgct gctccaggct gcccggcgga gcttttggct gaagcctcgc
     4141 ctgggttggg gccccggttc gcgtcaagat gctgcgaccg tgaacgtggc atttggtcca
     4201 cactcgatga taccagcggt aaagggggac atacgagccg gttatgctcg ggggggagat
     4261 cagaaatcct gcggaagcct gaggcggcca aatgggggcg atgggactgg cggaacgagc
     4321 ggcgctggct cccgagggcg ggcgtccggc cgcgcacagt gaaaccttgg gacgggcacg
     4381 aattacactc agcgacgtcg atagcgagcc gttccgagcg ctgccaatcc ggcggccagt
     4441 acgccgaatg agacaggctc cggcatggcc agggcgacgc tcacgttgtc aaggccggtg
     4501 tattccagtc cctgatcgcc aaggaacgat atcgtcgtcg tgggaccgcc agcaacgaaa
     4561 tcgaacgtct cagtctccca gttattggca ccggtatcgg cactggtgaa ggtctgcgaa
     4621 ctggagcctg cactggcagt gatgcccgat ggcagtccat agatggagga actgccaaga
     4681 tcgaacctca actggtacgt ggcgccggcg gttgttgata tcgtctggct aactccgccg
     4741 aagggtgcgc cgtgcaaata cccggtcaaa tcgacaaaat agccgccatt gctcgcggtc
     4801 agcaggaacg ggttgcctgg tccgatccag gacacaaagt ggctcccggt gacagtccag
     4861 ccggtcatcg ccgtcgaccc caggtcgaca ttcattgtgt cctggcccgt attgtcgagg
     4921 aagttggtcg catcttcgaa gctgccattg accagcagat tggccgcggc ctgggtacca
     4981 ggcaggcagg cagcagcaag tgccattatg gaaatgcaaa aaagtcgtgt tcgcacgaat
     5041 taactctcct cattatgcca acagtcataa ctacgatgat tcttaaagca aatatcaaag
     5101 caaatcgcac gccaatgtgg ttaataccgt aaatctaacg gtctatcctt ggcctggccg
     5161 atccaatcgc gtagcggtgt aagatattcc gacaaacgat cctggtagca ttaacggtct
     5221 gttgcctcga agttgtgccg agcctagtgg aagtcgtgac gtcctgctca tgcaggatgg
     5281 gcggccacgc cgagttcggc gaagcaggag ggccgacccg accagcgagg aaaagtcgat
     5341 gagcacgacc tggccggctg ttgcgcagtg actggctggt tatcgcggac ccgacgatac
     5401 aggcacggct gaaagcgatg tgtcgcagcg cggttgcctg gagaaggtgc cgcccgtgca
     5461 ggctgggatc gcgctgcagc cggcagcctg atctgcagag gcgtgctgac tgaggtgacg
     5521 aagttctgga atgtgcgaag aaaggcgtga agggggcgac tgtggccaac gcggctggat
     5581 gctgactggt acagctccat tatggaaggg gcgccagtct gccatcgatc gaactcccca
     5641 gtgatcgcaa gatatcgcgc aatcggttca gtgttcgtga cccagatcac agacatgcag
     5701 cggctcggtg gctaccccgg aaggggaccg gcatcggtaa ccacaggtgc cactacggac
     5761 gtatcgctcc aagatcagtc gggtgcgctg ttctcggctg acgatcatgc gcgaccgagc
     5821 aacaatgagt tgcgcgtcga ccagccaggt ccgggccaac aaatgaagtc caattggtga
     5881 cttgtgcgtc gaaatgattg gtcggatcgt atcgagcagc tgggagggct gaagaaccat
     5941 gagcgacctt tctgcggcgt cgcctgcttc atcaaacgat ggcacggata atgtctaccg
     6001 catcgcactc agcatgtcgg gtgctatatc cgccggggcg tatactgcag gtgtgctcga
     6061 ttttctcatg ctggcgctgg cggaactgga gcgggaaaag gctgcgaggc cagcgaggtt
     6121 cgtgccgccc aaccacgacg tgcgaatcgt ggcgatggcc ggggcctccg cgggtgggat
     6181 ctgcggaccg ctgagcacgc tggcgctggg tcatggaatg gtgttcccgc cggtggagat
     6241 ggaggtcgat ggaaagaagg atacgggcga aaagcagcgc atcttctgcg tccttccgag
     6301 cctgtacgcg gcgtgggtgg aaaagccgcg catggtggcg cgcaacgggg ttgatccgtc
     6361 gctgctgacc acggaggacc tggataacgg caaggtggtt tccgcgctca acgcgggcgt
     6421 cttgaacgac atagccaccg ccgcgttacg accgccggca cacccggcga cgatccaggc
     6481 gcctcacgaa tttcttgcga agccgctgca cctttacctg gccatcagca acctgccggg
     6541 cattcagtat gaggtgcacg gcagcagcga taaagacgtc tatcgcatgg tcaaccacgc
     6601 cgatcgtgtt cacatctgca tccgggatat cggcagcgat aggcgggtcg cgagcaagtg
     6661 ggcgggcacg gacaatccca gcgaaacgct cgatctggca tcgctgcaac aggatccgat
     6721 gaagtggaag gcgctggcga aagcgtccct ggcgacggct gccttcccca ttggcctatc
     6781 agccagggca ctgatcgcga aacaggaaat ctacgcaggc aggtggtttc ctgcgccgcg
     6841 gttcgggacc gctgcgattg gcccgacgtg gccccagtgg gaaccgccga tgcccgagcc
     6901 attcccgttc gtcaacgtcg atggggggat gatcaataac gatccgttcg aattcgcccg
     6961 ctactcgctg atcgatgact gggagacgcg cggcgcggcc aatccgtcag ctgcggagga
     7021 tgcggaccgc gcggtgatca tgatcgctcc gtttcccgaa ggcgcggtgg tgccgacggg
     7081 cggatacaac gatgacaccg ggctgctgag cgtgatcacg cgcctgttgc cgacgttgac
     7141 gcagcaggtg cggttcaagg taagcgaact cgctgccgcc gcggacccga catatgccag
     7201 ccgctggctg atcgctccgc gccggcgcga cagtcctgac gaggtgccgt cctcggacaa
     7261 tatcgcctgc gggttgttgg gcggcttcgg cgggttcctt gaccagcgct tccgcgagca
     7321 cgacttcctg ctgggccagc gcaactgcca gaactttctg cgcaactgga cggagagcaa
     7381 gttcaacaag acgtcgccta acgacccctg ggtcgttcgg ctgtgtggcc tggccctgag
     7441 caaggagata ccggctcccg actggccgcg catggagata gaggcgctga gcgaggtctc
     7501 gcgccgcatc aagatacgcg cgaaaaagct ggtgcctgcg ctggtgacgc aggaggttgg
     7561 ttcgcacttc gtacgttggc tgtttcgtgt cgtttggtgg tgggtgagag acaaggtgct
     7621 gaacatgatc aacggcatga tgctcgctga tctgctgcgc cgcgaccaga tcgaggatgg
     7681 catggccgac ggcagcaggg atcgactgcg gaatttcaca ccccaggagc gtggggtgtt
     7741 cgccgcactg gcccttgccg attacgacgc ccggaccgtg actgggctcg ccacgagcct
     7801 gaagctgacc gagtcacaga ttgacggcat catacaacgc gccagagccc tggatccggc
     7861 ggcggtgcac cacgtggtgc aatcccgcta caacagcaag gatggccagc cgtcttatac
     7921 cctggcccgc cgcaggctgt ccgcatggaa gaaattcagc gcggcaatgc cttggttgaa
     7981 tcgggttgcg ggagtgaaaa ttggctgacc acgtgcgtgc cgtgacgctt cacgcgtcct
     8041 ggcgcaagtt ccggccggca gcaattggtc tggttcaacc gagcgcatct gtggttcgcg
     8101 cgtgccactg ccatcgtggc agactcgggc tctcgcacta aaacaacgtc cggataacaa
     8161 ggacggccgc gcttgatcgc ccagctcacc ggacgcgtcg aagcgctgga agacggacgc
     8221 tgcgtcatcg atgtgaacgg cgtcggctat ctggtgcacg cctccacccg gacgcttgcc
     8281 gcgctgccgg cgccgccaga gggcgcgcgg ctgctggtgg agacgcatgt gcgcgaggac
     8341 gcgatcgtgc tctatgggtt catcgacccg gccgagcgcg agtggttccg gttgctgacc
     8401 acggtgcagg gtgttggcgg gcgggtggct ctgtcggtgc tgtcggcgct ttcgccggcg
     8461 gacctggtgg cggcgattgc gtcgggggat cgggcgagcc tgacccgggc gcccggagtg
     8521 ggcgcgcggc tggcggtgcg gttgctgacg gagctgcggg acaaggcggg agcgatgccg
     8581 acggcgcccg ggattgcggc ggtgccggtg gtcgcttcac ggggcccggg cgaggatgcg
     8641 atgtcggcgc tcgtcaatct tggctaccgt cggatggagg ttcagccggt ggtggcaagg
     8701 gtgatcgagc ggctgggtga ggtggcgacg gtggatgcgg tgctgcgcga gagcctcagg
     8761 gagctggcgc agagggttgg cggatgagtg ggcgccgccc ccagccgtcc tctccggcga
     8821 tcggggggga agaccgtgcc gtttcggcgg aacgacggga ggacgatgcg gcggaggtca
     8881 gtctgcggcc ccagtcgctt gaggagttcg tcggccagca ggccagccgc gaaaatctgg
     8941 ccgtgttcat cgccgcggca cgggcgcggg gcgaggcgtt ggaccacgtg ctgctgcatg
     9001 gtccgcctgg actgggaaag acgacactgg cgcagatcgt ggcgcgggag ctgggggtgg
     9061 ggttccgggc gacgtcgggg ccggtgatcc aaagggcagg ggacctcgcc gcgatcctga
     9121 caaacctcca acccagggat gtcctattca ttgacgagat ccaccggctt cagcctgcca
     9181 tcgaggaagt gctctacccg gcgatggaag acttccagct cgacctgatc atcggcgagg
     9241 ggccggccgc gcgctccgtg cggatcgacc tggcggcgtt cacgcttgtc ggcgccacca
     9301 cgcgtgccgg gctgctcgcg acgccgctgc gcgaccgttt cggcatcccg ctgcgcctgg
     9361 ttttctacac gccacaggaa ctggagctga tcgtgcggcg cggcgcggcg aagctcggcg
     9421 tgcccctgac gcaggatggg gcggccgaga tcgcacggcg ttcgcgcggc acgccgcgga
     9481 ttgcggggcg cctgctgcgg cgagtgcgcg actttgcctc ggtcgccggc gtggaggaga
     9541 tcgaccgggc gatggcggac aatgcgctca atcgactgga ggtggacgcg ctggggctgg
     9601 atgcgatgga ccggcgctat ctgcggcgaa tcggggagca ccacggggga gggccagttg
     9661 gtgtggaaac gctggcggcc gcgctggcgg aagcgcgcga caccctcgaa gatgtgatcg
     9721 aaccgttcct gatccaggaa ggtctggtgc tccggacaag ccgcggccgg atgctggggc
     9781 agagcgggtg gcgtcacctc gggctggtgc cgccggcgcg ccagcccgat cagttcgacc
     9841 tgttgcctga atgaatggac atgcagtctc cattccggcg gatggccagc accgctacac
     9901 gctgcgcgtg tactacgagg atactgatgc cggcggggtc gtctatcacg cgacctacct
     9961 gcggttcgct gagcgggcgc ggaccgaggc gttgcgcgcc gcgggcgttc cgcacgcgga
    10021 attgcttgca gatcatggcg tgatgttcat ggtgcggcgc atcgaagtgg actatctccg
    10081 tccggcccgc ctcgacgatt cgctgaccgt tgtaacggaa ccgctcgcgg tgggcggcgc
    10141 gagcgcgacg ttgcgccagg atgtgctagg cccagatggg ccctgcgcga ttctgacagt
    10201 gcggcttgcc tgcgtgaagc caggggatgg acggcccggg cggctgccgc aacgttggcg
    10261 gacagcgctg acggcgatgt gcaacgcggc gactgccgct cgaaacgagg agtagctgga
    10321 gtggatcggg cagtggacgc ggcaaacctc gctgtgccgg gggcggatct gtcgctgtgg
    10381 ggcctcttta tagaggccga tatcgtcgtg aagatcgtca tgctcgggct gctggcggcg
    10441 agcgtgtggg tgtgggccgt ggtgttcgag aaatggtcca gcctgcgcaa ggtgaaccgc
    10501 gaagcggacg ggttcgagga tcgcttctgg tcgggcggca gcctggagga attgttcgat
    10561 cacgaaggca ccaatccggt gcatccgatg gcggcggtgt tcggtgcggc gatgggggag
    10621 tggcgccgct cggtgcgggt ggcgggggcc gatatcagcc ggacttcggt gcgcgaacgc
    10681 gtggacaggg cgatcaccgt cacggtccag cgcgagatgg accggatgga acggtggatg
    10741 atcttcctcg cgtcggtggg tgcgacggcg cccttcatcg gcctgttcgg cacggtgtgg
    10801 ggcatcatgc attcgttctc ggcgatcgcg gcgatgcaca acacgaacct cgcggtggtg
    10861 gcaccgggca ttgccgaggc gctgttcgcg accgcgatcg gtctggtggc ggcgatcccg
    10921 gcggtgctgg cctacaacaa gatcagcaac gacctgacgc ggttcgcctc gcggctggaa
    10981 gggtttggcg ccgagttcag cgccatcctg tcgcggcaga gcgaggaacg ggcgtaaccg
    11041 atggcgctga ccttcaacac gcgcggcaac ggccgtggcc ggtatcgtcc gctgtcggag
    11101 atcaacgtca cgccgctggt cgacgtgatg ctggtgctgc tgatcatctt catggtgacg
    11161 gcgccgctga tgacgtcggg cgtgtcggtc gatctaccca aaacggacgc aaagcccatc
    11221 aatacggaca gccagccgct gacggtatcg ttggatgcac agggcaagat cttcctgcag
    11281 gatcaggagg tgcagcttcc cgagttggtc acgaaactgc aggcgatcgc gcagaacaat
    11341 cctgatcggc gtatattcgt tcgtggtgac aaggacttgg cgtatgggcg gatcatggaa
    11401 gtgatgggca cgatcaccca gggcggcttt accaaggtgg ctctgctggc ggagcagccg
    11461 accgtgacgc cgtcgacgcc gcccgccccg acggcgcccg caccggtgcc tgcgcccagc
    11521 ggccagacca aaagccgcta ggaaacaccg cgtgctgcaa cgcgtcctcc gcggcacgga
    11581 tcgactcgat cgccgcaatg aaggggtcag cccgacgctg cgggttggcg cgcttatttc
    11641 cggtggtgtc catcttgcca ttctgttgtt gttgctgatc gggatcccgt tcagccggcc
    11701 gcccgcggag ccgccggaag aaacatccgt gtccatggtg ttcggcactg ccgacagcgc
    11761 gatgaaggcg ccggcgccgg ctgcggtccc ggcgccggcc aatacgcaga ccaccaatcc
    11821 ggcaccgaag gccaccgagc cgccgaagcc gcagcctatc gaggcaccgc cgcctccgcc
    11881 gcccccgcct ccgccgccgc ccactccgcc gcagtcgacg gcgccgccgc tgccgacgcc
    11941 gcccactccg gtgccgccgc ccacgccggc gccggcagcc gagaccgcgc caccaccgcc
    12001 ggccgcgccg ccaacgccgc agcaggcgca accgccgctg ccgacgccgc cattgcccgt
    12061 gccgccacct ccagtgccgc caccgccgtc accgcaaagt gccacgtcgc agccgaatgt
    12121 gacgaagaac ccggcgcccg aaagcgatgc gctggagaac accctgtcaa agctgcgcag
    12181 cctgcagaag cagacccagc cgccgaaggc acgctacaat ccgcagtcgg gcggcgcgcc
    12241 gaatggcgga ggcaatccgc tgagcgatga cacttcggcg ctgtcgatgg cgcaacgcgg
    12301 tgccatcggc gacttcgtgc ggcgctgctg gagcaccgat cccgggatgc tcgatctcga
    12361 caggatgcag gtgttgttga cggtgaccac tgaccccagc ggcgttgtgc ggcgcgccgt
    12421 ggtggccgac gacgatgccg gccgtgttgg ctcggacatg cggctgcggg tatttgccga
    12481 gcgcgcgatc cgtgccgttc tcgatccgaa ttgctccaac ctgccgctgc cgcagaacat
    12541 gcttgggcag acccgcgtat tcaccttccg attccgaccg tgagagggct cacttaagga
    12601 aaggctctga aatgatggat ttctccttgc cgtcccttcg tcgccgttca ctgctgaccg
    12661 gcgctgccgg aggcgccgcg ttgtttgcgg ctcctgccat ggtgcgcgcc cagggagtgg
    12721 cgccgccgcg cgcgccggcg cctggcgcgc aaggaggcgg ccagacggca gtgatcgacg
    12781 tcaatcgggc gcggaccgat ccgattccga ttgcgctgcc agcgttggtt ggcagcgacg
    12841 gcgcctcggc ccagcttggc caggacatca tgggcgtggt gagcagtgac ctcgggaaca
    12901 gcgggctgtt ccgtccggtc aatccctcgg cgttcattca ggcgtcggca ggacctgcgg
    12961 ccgatatgcc gaactttgcc aactggaagg caatcggcgc gcaggcgttg gtgacggggc
    13021 gcgccgaatc gcagggcggt ggacagatcc gggtggagtt ccgcctgtgg gacgtgctgc
    13081 cggggacgca gcttcagggc actgcctaca caaccacgcc agccaactgg cggcgcatcg
    13141 cacacatcat cgctgacgtg atctatgagc ggctgcttgg cgagaagggc tatttcgata
    13201 cccgcatctg ctacattgcc gcgaccggac cgcgcgaccg gcggacgaag cggctggcga
    13261 tcatggacca ggacagtgcc aacaaccggt tcctgaccga cggttcgtgg ctcgtgctga
    13321 cgccgcgctt ccatcccacg cgcgacgaga tcgcgttcat gagctacgcg aacaatcggc
    13381 cgcgcgttta catcttcaat ctcggcagcg ggcagcagcg cgtgctgggc gattttccgg
    13441 gcatgacctt cgcgccgcgg ttctcgccgg acggcaacag cgtgatcatg tcggtagtga
    13501 acggtggcgg gtcggacatc gtgtcggttg atctggggag ccacgcgtca cgccggctga
    13561 ccaattctgg ggcgatcgac gtcagcccct gctacagccc ggacgggtcg cagatcgtgt
    13621 tcaactccga tcggggcggt gaccagcaac tctacatcat gagcgctggc ggcggtgggg
    13681 cgaagcggat cagctacggc cagagccgct atgcgacacc ggtgtggtcg ccgcggggcg
    13741 atctgatcgc cttcacacga tttggcggcg aggtgggcaa cttcgccatc ggcgtgatgc
    13801 atccggacgg gtccggcgag cggatcctgt ccgagagctt ctcggtcgaa ggcccgacgt
    13861 ttgcaccgaa cggacgggtg ctgatgttct ggcgccaaag cgcggcgcgg gacagccgag
    13921 gcagtggatt ttcttcgcgt ctggtgtcga tcgacatcac cggcttcaac gagagggtgg
    13981 tcgagacacc gacggacgct tcagatccgg cctggtcgcc tctggcggcc tgaactgtgc
    14041 atacgcggac atgattgata tgtaaaaacg ccggttctcg cgaacttgac cggtttcaat
    14101 gcaaccagct aatcgcaaag ccgggcggaa cgggataccg taattcgctc ggcgtgactt
    14161 ctgcttctgg cgacaccccc caatgaggga cgaactacca atgaacagca agattctcgg
    14221 caccttggcc gtcgtcgccc tgttggcggc gtgctcggac caaaagacga ccgcttcgac
    14281 cggggcgggt gcgaccacct ccggcccggt accgggtagc gaggaagacc ttgtcgccaa
    14341 tgtcggcgac cgcgtgttct ttgccttcaa cgcgtcgaac ctggcgccgg atgcgcaggc
    14401 gacgcttgac aagcaggcgg ggtggctcgg caagtatcag caagtcaatg tgcaggtcgc
    14461 ggggaactgc gacgatcgtg ggaccgagga atacaatctg gcactgggtc agcgtcgggc
    14521 caattccagc cgcgactacc tcgttgccag gggcgttgcc gctgcacgga tcaccacgat
    14581 cagctacggc aaggatcggc cgactgctat gggcgacaac gagcaggcct gggcgcagaa
    14641 ccgcaacacg atcaccgctg tccgctgagc gccggctggg ctgaggcgat gcgatacgaa
    14701 ccggcggccc tgtggccgcc ggtttcgttt gcgcgctgtc tgccatatca ccgcccgcgt
    14761 ttggcatcag aacgccgagt ccggaggcgc atcgcatgag tatcccgtcg gcacttcgtc
    14821 tgatcctggc cgctatagtg ttggtctacg tgacacccgc gtgggcgcag gtggagagcc
    14881 gggaaggcat cgcgttgcag aaccagatcc tggaactgca gcgccaggtg cagggcctgc
    14941 aggcgcaggg ggcgcgcggc ccgaccaatc tgggcggcag ctattcctca ccaccctcga
    15001 cgagcagtgg cgatctggta gcgcagttgt tgtcgcgggt cagtacgctg gaggaccagg
    15061 tgcgccagct ccgtggccgg gtcgatgaat tgcagaatca gcaggaccgg cagtcggcgg
    15121 atctgagtaa gaagatggac gatctggctt tccagcttca gggctccgcg ggcg
//
LOCUS       DATGEM010000703         7223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_1696481, whole genome shotgun sequence.
ACCESSION   DATGEM010000703 DATGEM010000000
VERSION     DATGEM010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696481"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VKQ27_21640"
     CDS             complement(<1..1392)
                     /locus_tag="VKQ27_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLY91600.1"
                     /translation="MIITERTRTTYAPGRAIEDAVSWDPPARIVAAIFIAFVLGMACY
                     YAFVEAPTAIKHDMSEAYAWGQEFQLGYNQHPPFWAWLCALWFEVFPHTQWAFGLLSA
                     LNAGIGLLGAWHLIGDFARGPKRVAAWALLILTPLYTAYAYKYNANIIFISIWPWTAH
                     YFMRSVQTRRLGDAVGFGICVAFALLSKYYAVILVASCFLALLQHPARWRYLASVSPY
                     LSVAVTVALFAPHAWWLLANDAPPLRYLSEVSDQRWPLVLGHTWRTFSGAIGMNLSVV
                     AVVLIGGWLARRKGETEPMREIRGPMFGVLVTLALAPVVLTLASGLVLRNPLTDEMTV
                     GIFPLLPLLVIELVGRGRVERLGRLSVHLATALMVGAILISPAVAAVRTWFSPVAKDV
                     MPFEEVALDATKLWHERTGEPMGFVAGSPWFENATAFYSPDHPSAFAYFDYSRSLWVT
                     PERLAKRGLLSICH"
     gene            complement(1509..2174)
                     /locus_tag="VKQ27_21645"
     CDS             complement(1509..2174)
                     /locus_tag="VKQ27_21645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLY91601.1"
                     /translation="MGNTINLTAADGFSLSAYTAGSAGGTKGLVVIQEIFGVNHHIRD
                     MCDRFAAQGYAVCAPALFDRAEKGVELGYTQNDIDNGRAYRMKLDDAKVMADVEAAAK
                     HLAGKKLGIVGYCFGGTVAWWGATRSRSFAASSCWYGGGIAGTRDERPNCPVQMHFGE
                     KDASIPMTDVDAVRAAQPKVESYVYMGAGHGFGCDERGSFSKPDYDLAQQRTLEFFAK
                     HLG"
     gene            complement(2453..2950)
                     /locus_tag="VKQ27_21650"
     CDS             complement(2453..2950)
                     /locus_tag="VKQ27_21650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HLY91602.1"
                     /translation="MIDPTALPYRRNVGAALFNRAGKVFVARRADLPNAEGAPGGWQL
                     PQGGIDRDEDPRAAVLRELEEEIGTARAEIIGEHPEWLTYDLPAELIGVALRGRYRGQ
                     QQRWFALRFTGNDSDIRLDLDPHPEFDAWRWTDLAELPALAVDFKRPIYQILATAFAR
                     FAATP"
     gene            complement(2947..4035)
                     /locus_tag="VKQ27_21655"
     CDS             complement(2947..4035)
                     /locus_tag="VKQ27_21655"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HLY91603.1"
                     /translation="MGLSTVFAGWKGLARFWSLVVLLLAAGGSTLQLLGPLPEPHREA
                     VAAAPPSVAPQTPPQTAPSQPNPPPGQAQQAASISPAQRPGRSTPGPIVDPDPALLEP
                     VPGSTSDMLPRVAEDGRMPMQVYAAGFDASSRRPRVGLLLAGIGLNQTDSAAAIHSLP
                     GGITLAFPPYAQNLGKLLTDTRFAEHEFLMSIPMEPQGFPHNDPGQHALMTNLSFEEN
                     HARLIWALSQLRGYVGATGALASGLLGERFASLPEELQPVLTELAQRGLLYIDPRVGA
                     PRLPLVWSRSIDFIIDEPDSAAAIDDKLNQLSKLAHSKGTALGLATAPRPITIQRVAA
                     WADGLTADGLALAPVSALVGQPAKGTER"
     gene            complement(4081..5490)
                     /locus_tag="VKQ27_21660"
     CDS             complement(4081..5490)
                     /locus_tag="VKQ27_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLY91604.1"
                     /translation="MDYRSRRFRTGLLLGTAFLAGVAIGPGSGLIARQLGYHFGITSA
                     FAQDSSRADTYRLLTLFGDVFERVRADYVDQVNDKDLIENSINGMLTGLDPHSSYMNA
                     KQFRDMQVQTKGEFGGLGIEVTQDNGFIKVISPIDDTPAAKAGIKAGDIITALDGKTV
                     EGLSLQDAVDKMRGAPNSKITLTIKREHIDKPIEVSMLREIIHIQVVKSRLEPDNIGY
                     VRLSQFTEQADAGIRDAVKQLRQKAGGKLTALILDLRNNPGGLLDQAVAVSEDFIPQG
                     EIVSTRARHSEDSQRWDAKGDDIINGVPLIVMINGGSASSSEIVSGALQDHHRAVLLG
                     TRSFGKGSVQTVIPLPGNGAMRLTTARYYTPSGRSIQGQGIAPDVKVAESRDAEARFG
                     PEREADLNHILTNQGGTPSTAAAPRTDLPPMAKDIPDKPPEGFPKFDAAKPDATDFQL
                     QQAVVLAKAMAAQSSVSAK"
     gene            complement(5605..6864)
                     /locus_tag="VKQ27_21665"
     CDS             complement(5605..6864)
                     /locus_tag="VKQ27_21665"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLY91605.1"
                     /translation="MRRARRSSLFALIALTTVAAQPARPDKEAATRQQIEQADRQRAA
                     EQAAEQAATARAAAAADDERRLAAGRIAAAAKLRGAEAATEQAADAMRHLAEEKQVAQ
                     EALNKRAATMEPLLPLIERLSLFPVETLLAVPGKPEDTLRGVLVLRGLANEVEQEAEA
                     LKRDQARVDAAIKALQAEAPKLAAAQARQQAQAAALDEQIAAATAGQKQAQSDATAAA
                     RNAQDAAAKADTLRGALAELVAQRAAEAERARADAARAAQQKHADQQAAAEARLAATR
                     SPSGATALASAAQPHGQLTTPVAGPVVRAWGDATAGGPSNGISYRAAPDARVVAVCNG
                     RVAFAAPFRSYGLLLILDCGGGYHTVLAGFERLDVKVGQTVAAGEPVGVMPGWDPAST
                     GDRPTLYVELWREGKPVNPAPWLRASG"
     gene            complement(6842..>7223)
                     /locus_tag="VKQ27_21670"
     CDS             complement(6842..>7223)
                     /locus_tag="VKQ27_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLY91606.1"
                     /translation="LIVLNFANPDMVGHTGSLPAAIKAVETVDAGLGRIAAAVQKQGG
                     ALLVTADHGNCELMRDPTTGGPHTAHTTNPVPVFLMGGAASAIKDGRLADVAPTLLQL
                     MGLKQPQEMTGHSLIRADAARAAE"
BASE COUNT         1212 a         2356 c         2393 g         1262 t
ORIGIN      
        1 atggcagatg gacagcaatc cgcgctttgc caggcgctcg ggtgtcaccc aaaggctgcg
       61 gctatagtcg aaatacgcaa acgccgaggg gtggtccggg ctgtagaacg ccgttgcgtt
      121 ctcgaaccag ggagaacctg caacaaagcc catcggctcg cctgtccgtt cgtgccaaag
      181 tttggtcgcg tcgagggcca cctcctcgaa cggcatcacg tccttagcca ccggtgagaa
      241 ccaggtccgg accgccgcga ccgcgggcga gatcaggata gcccccacca tcagcgccgt
      301 cgccagatgc acgctgaggc ggccgagccg ttcgacccgg ccgcgaccaa ccagttcgat
      361 caccagcagc ggcagcagag gaaaaattcc cacggtcatt tcgtctgtga gaggattgcg
      421 cagcaccagg ccgctggcca aggtcaggac cactggtgcc agggccagtg tcaccagcac
      481 gccgaacatc gggccgcgga tctcgcgcat cggctccgtc tcgcccttgc gccgtgcgag
      541 ccagccaccg atgagcacga cggcgacgac gctcaggttc attcctattg cgccggaaaa
      601 ggtcctccat gtgtggccga gcaccagtgg ccaccgctga tcggagactt ccgacaggta
      661 acgcaacggc ggagcgtcat tggccagcag ccaccacgcg tgcggcgcga acagcgccac
      721 ggtcaccgca actgagagat acggagatac ggacgccagg tagcgccagc gggcagggtg
      781 ctggagcagt gccaggaagc aggaggcaac caggatgaca gcatagtatt tcgacagcag
      841 cgcgaaggcg acgcagatgc cgaagccaac cgcgtctccc agccggcggg tttgcacgga
      901 ccgcataaag tagtgagcgg tccacggcca gatcgagatg aaaatgatat tggcgttgta
      961 cttgtaggcg taggcggtat agagcggcgt caggatcagc agcgcccagg cggcgacgcg
     1021 cttgggacca cgggcgaagt cgccgatgag gtgccacgca cccaacagcc cgatgccggc
     1081 gttcagtgcg ctcagcagcc cgaacgccca ttgcgtatgc ggaaacacct cgaaccacag
     1141 ggcgcagagc caagcccaga acggcggatg ttggttgtag ccgagttgga attcctggcc
     1201 ccaggcataa gcttcggaca tgtcgtgctt gattgccgtc ggagcttcaa cgaacgcgta
     1261 atagcaggcc atgcccagga cgaaggcgat gaagattgct gccactatgc gtgctggcgg
     1321 atcccaactg acggcatcct cgatcgcacg gcccggcgcg tacgtcgtcc gggtccgctc
     1381 tgtgatgatc attctgtcgt aattctctga atcagggcag cgacgatgac aacgggctgg
     1441 gcccgcttgt caacagagca gacggttcgc cgcgcgcgag cgcgcaggat cgcgcgcggc
     1501 gacaggcgtc aaccgagatg tttggcgaag aattcgagag tacgctgctg ggccagatcg
     1561 tagtcgggct tgctgaagct gccccgctcg tcgcagccga agccatggcc ggcgcccatg
     1621 tagacgtacg actcgacttt cggttgggcg gcgcggaccg catcgacgtc ggtcattggg
     1681 atcgacgcat ccttctcgcc gaaatgcatc tgcaccgggc agttcggacg ctcatccctg
     1741 gtgccagcaa tgccgccgcc ataccagcag gacgacgccg caaagctgcg gctgcgcgtt
     1801 gcgccccacc aggcgacggt gccgccgaag cagtagccga cgatgccgag ctttttgccg
     1861 gccaagtgtt tagccgcagc ctcgacatca gccatcacct tggcgtcgtc gagtttcatg
     1921 cggtaggcgc ggccattgtc gatgtcattc tgggtgtagc cgagctcgac gcctttctcg
     1981 gcgcggtcga acagtgccgg cgcgcacact gcatagcctt gggcagcgaa gcggtcgcac
     2041 atgtcgcgga tatggtggtt gacgccgaaa atctcctgga tgaccacgag ccctttggtt
     2101 cctcctgccg aaccagcggt gtaggcggaa agcgagaatc cgtcggcagc agtcaggttg
     2161 atcgtgttac ccatgaagct cctcctgttg ttcggggtat ccccgacccc caggatggcc
     2221 ggttcggcga gggcgtcaac cttgggatca tagcctccgc cggacgacct tacgcggcgc
     2281 gatctgtcga gaggggatga aattgggacc acaaaacacg cgtaacacgc tgcgggcagt
     2341 cgtgccttac ggtgcgagga tgatgggtta gacgactgag tatgcggcat attcatcggc
     2401 gagacgtcac atgcggcaag caacgcagtc cacctgtcct gtctcggttc ggctacggcg
     2461 ttgccgcgaa gcgcgcgaag gcggtggcga ggatctggta gatcggccgc ttgaagtcca
     2521 ccgccagggc tggcagttcg gcgagatcgg tccagcgcca ggcatcgaat tcggggtggg
     2581 gatcgaggtc gagccggatg tcactgtcat tgccggtgaa ccgcagcgcg aaccagcgct
     2641 gctgctggcc ccggtagcgc cccctcagcg ccacgccgat cagctccgcc ggcagatcgt
     2701 aggtaagcca ctccggatgc tcgccgatga tctcggcgcg cgcggttccg atctcctcct
     2761 ccagttcccg cagcaccgcg gcgcgagggt cctcatcccg atcgatcccg ccttgcggca
     2821 gttgccagcc accaggtgcc ccctcggcat tcggcaaatc cgcgcgccgc gccacgaaca
     2881 ccttgcccgc ccgattgaac agcgccgctc cgacattccg ccgatatggc agcgcggttg
     2941 gatcaatcat cgttctgttc ccttcgcggg ctgtcccacg agcgcgctga cgggagccaa
     3001 cgccagccca tccgcggtaa gtccgtcagc ccaggcagcg acccgctgaa tcgttatcgg
     3061 tcgtggcgct gtcgccagcc caagtgctgt gcccttgctg tgtgccaact tgctgagctg
     3121 gttcagtttg tcgtcgatag ctgcggcgga gtctggttcg tcgatgatga aatcgattga
     3181 ccggctccac accagtggca gccgcggcgc tccgacgcgc ggatcgatat acagcaatcc
     3241 gcgttgcgca agctcggtca gtaccggctg aagctcttcc ggcagtgagg cgaaccgctc
     3301 gccgaggaga ccgctggcta gcgcgccagt ggcgccgaca tagccacgca gctgcgacag
     3361 cgcccaaatg agccgagcgt gattttcctc gaatgaaagg ttggtcatca gcgcgtgctg
     3421 cccgggatcg ttgtggggga agccttgcgg ttccatcggg atcgacatca ggaactcgtg
     3481 ttcggcgaac cgcgtatcgg tcaggagttt tcctagattt tgcgcgtagg gtggaaacgc
     3541 cagcgtgatg ccgcccggta aagaatgaat ggcggcggcg ctgtccgtct ggttcaggcc
     3601 gatgccggcg agcagcagcc cgacgcgggg acggcgcgaa ctggcatcaa atcctgcggc
     3661 gtagacctgc attggcatgc ggccatcctc cgccacccgc ggcagcatat cggatgtaga
     3721 gcccggcacc ggttccagga gcgccggatc aggatcaacg atcgggcccg gggtgctccg
     3781 gccggggcgt tgtgctggtg aaatggacgc tgcctgctgt gcctgtcccg gcggaggatt
     3841 aggctgcgat ggagcggtct ggggcggggt ttgcggcgcg acggacggcg gcgcggcagc
     3901 cacggcttcg cgatgcggct caggcaacgg cccgagaagc tggagggtgc tgccccccgc
     3961 cgccagcaga agcaccacca gcgaccagaa ccgcgccagc cccttccagc ccgcaaacac
     4021 agtgctcaga cccaaccctg gcatcgccat tcccccgcgc gccgccaagg cccgcgagcg
     4081 ttacttggct gagacgctgc tctgcgccgc cattgccttc gcgagcacga ccgcctgctg
     4141 caactggaaa tccgttgcgt cgggtttggc cgcatcgaat ttcgggaagc cctcgggcgg
     4201 cttgtccgga atgtccttgg ccatgggcgg aagatccgtc cgcggcgccg ccgccgtgct
     4261 gggcgtgccg ccctggttgg tcaggatatg gttcagatcg gcctcgcgct cgggaccgaa
     4321 ccgtgcctcc gcatcgcggg actcggccac cttgacgtcc ggtgcgatgc cctggccctg
     4381 gatcgagcgg ccagacggcg tgtagtagcg cgccgtggtc agccgcatgg cgccgttgcc
     4441 gggcagcggg atgacggtct gcactgaccc cttgccgaag ctgcgcgtgc caagcaggac
     4501 ggcgcggtgg tggtcctgca gggcgccgga gacgatctcg ctggacgacg cggacccgcc
     4561 gttgatcatc acgatcagcg gtacgccgtt gatgatgtca tcgcccttgg cgtcccagcg
     4621 ctgcgaatcc tctgaatggc gcgcgcgcgt ggagacgatc tcaccctgcg ggatgaagtc
     4681 ctccgacacc gcgacagcct ggtcgagcag tccgcccgga ttgttgcgca gatccaggat
     4741 cagcgcggtg agcttgccgc ccgccttctg cctcaactgc ttgacggcat cgcggatacc
     4801 ggcatcagcc tgctcggtga attgcgatag ccggacatag ccgatgttgt ccggttccag
     4861 ccggctcttg accacctgga tgtggatgat ctcacggagc atcgaaacct cgatcggctt
     4921 atcgatgtgc tcgcgcttga tggtgagcgt gatcttggaa ttcggcgcgc cgcgcatctt
     4981 gtccactgcg tcctgcagcg aaaggccttc gaccgtcttg ccgtccagcg cggtgatgat
     5041 gtcgcccgcc ttgatgccgg ccttggcggc tggcgtgtcg tcgatcgggc tgatcacctt
     5101 gatgaagccg ttgtcctgcg tcacctcgat gccgagcccg ccaaactcgc ccttggtctg
     5161 cacctgcatg tcgcggaact gtttcgcatt catatagctg gaatgcgggt cgagcccggt
     5221 gagcatgccg ttgatggagt tctcgatcag gtccttgtca ttgacctggt cgacgtagtc
     5281 ggcgcgcact cgctcaaaca catcgccgaa caaggtcagg agcctgtagg tgtcggcgcg
     5341 gctgctgtcc tgggcgaaag ccgaggtgat gccgaaatga tagcccagct gccgggcgat
     5401 caggccggag cccggaccaa tagcgacgcc agcgaggaag gcggtgccta acaaaagacc
     5461 ggtgcgaaaa cgccgggaac gatagtccat tctgctcatc ctttaagcgc tgcctgtggc
     5521 cagacgcaat tgcccaattc cagtatcatt tgtcgcgcgc gtgtggctcc cgcgtccagt
     5581 attcaaatct tatcattatg acagttaacc actcgctctc agccatggcg ccgggttcac
     5641 tggtttccct tcgcgccaca gctcgacgta gagcgtcggc cggtcgccag tggatgctgg
     5701 gtcccagcct ggcatcacgc cgaccggctc gccggcggcc acggtctgcc cgaccttgac
     5761 gtcgagccgc tcgaagcctg ccagcactgt atggtagccg ccgccgcagt ccaggatcaa
     5821 taacaacccg tagctgcgga aaggcgctgc gaacgccacg cggccattgc agacggcgac
     5881 cacccgtgcg tctggcgcgg cgcggtaaga gatgccgttc gagggaccgc cggcggtggc
     5941 gtcgccccag gcgcggacga cggggccggc aaccggcgtc gtgagctgtc catggggctg
     6001 ggccgcgctg gcgagcgccg tagcgccgga aggggatcgc gtcgcggcga ggcgggcctc
     6061 cgccgctgcc tgctggtccg cgtgcttttg ctgcgcggcg cgggccgcat cggcgcgtgc
     6121 gcgttcggcc tccgccgcgc gctgggcgac cagctctgcg agcgcgccgc gcagggtatc
     6181 tgccttggcc gcggcatcct gcgcgttccg ggcggcggca gtggcgtcgc tttgtgcctg
     6241 cttctgacca gccgtcgcgg cagcgatctg ctcgtccagg gcagcggcct gggcctgctg
     6301 ccgggcctgc gcggcagcca gcttcggcgc ttcggcctgc aacgccttga tcgccgcatc
     6361 gacccgcgcc tggtcgcgtt tcagggcttc ggcttcctgc tccacctcgt tggcgaggcc
     6421 gcgcagcacc agcacgccac gcaaggtatc ttccggcttg ccggggaccg ccagcagcgt
     6481 ctccaccgga aacagcgaga gccgttcgat cagcggcagc agcggttcca tggtggccgc
     6541 gcgcttgttg agcgcctctt gcgccacctg cttctcttcg gcgaggtgcc gcatcgcatc
     6601 tgcggcctgc tcggtggccg cttccgcacc acgcagtttc gcggcagcag cgatgcgacc
     6661 ggcggcgagg cggcgctcat cgtcggccgc cgccgcggcg cgggcggtgg cggcctgttc
     6721 ggcagcctgc tcggcggcgc gctgacgatc ggcctgctcg atctgctggc gggtggccgc
     6781 ttccttgtcc ggccgcgccg gctgggcagc gaccgtcgtg agggcaatca gggcgaaaag
     6841 actactccgc cgcgcgcgcc gcatcggcac ggatcaggga atggcccgtc atctcctggg
     6901 gctgcttcag ccccatcagc tgcagcagcg tcggggccac gtcggccagc ctgccgtcct
     6961 tgatcgctga cgccgcgccg cccatcagga acaccggcac cgggttggtc gtgtgcgcgg
     7021 tatgtgggcc gccggtggtg gggtcgcgca tcaattcgca gttgccgtgg tcggcggtga
     7081 ccagcagcgc gccgccttgc ttctgcaccg cggccgcaat gcgcccgagc ccggcgtcca
     7141 ccgtctccac tgctttgatg gcggcgggca ggctgcccgt gtgcccaacc atgtcagggt
     7201 tcgcaaagtt cagcacgatc agg
//
LOCUS       DATGEM010000704         8798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Acetobacteraceae bacterium isolate SMAG_U9702
            k141_78357, whole genome shotgun sequence.
ACCESSION   DATGEM010000704 DATGEM010000000
VERSION     DATGEM010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965271
            Sequence Read Archive: SRR5579859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acetobacteraceae bacterium (soil metagenome)
  ORGANISM  Acetobacteraceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Acetobacterales; Acetobacteraceae.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 10:32:56
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,342
            CDSs (total)                      :: 4,315
            Genes (coding)                    :: 4,275
            CDSs (with protein)               :: 4,275
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 40
            CDSs (without protein)            :: 40
            Pseudo Genes (ambiguous residues) :: 0 of 40
            Pseudo Genes (frameshifted)       :: 10 of 40
            Pseudo Genes (incomplete)         :: 28 of 40
            Pseudo Genes (internal stop)      :: 8 of 40
            Pseudo Genes (multiple problems)  :: 5 of 40
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Acetobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78357"
                     /isolate="SMAG_U9702"
                     /isolation_source="soil"
                     /db_xref="taxon:1909293"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VKQ27_21675"
     CDS             <1..419
                     /locus_tag="VKQ27_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HLY91607.1"
                     /translation="FIYSPAMEKFKGKPWSFDALNEWLYKPSAYVPGTRMSFAGITND
                     KQRADVIAYLRTLSDSPVPLPAAKPAAAPAAPAPAAAGAPATAAPAPAAPAGNTNAVQ
                     PNAPAASPTPAGAPGTGPAAGGSASAPAAPNPPAKP"
     gene            423..1199
                     /locus_tag="VKQ27_21680"
     CDS             423..1199
                     /locus_tag="VKQ27_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLY91608.1"
                     /translation="MEDLDRFIAGAEAAADVAGAVVRPFFRAPVEVVSKSDRSPVTIA
                     DRSAEQAMRAVLAERFPDHGILGEEYGEDRPGARYRWVLDPIDGTRAFVSGRPTFGTL
                     IALMDGPAPIIGIIDQPISAERWVGARGRRTIFRGSYPGQVGCRPCPSLAEAELSCTS
                     PEMLGPDSARWQRLAAAVRRNYWGGDCYAHGLLAIGQIDIITESTMKLWDWAALVPVV
                     EGAGGKVTDWSGQTLRPDGDGRVLSVGDPRLLPQAVALLK"
     gene            1325..3295
                     /locus_tag="VKQ27_21685"
     CDS             1325..3295
                     /locus_tag="VKQ27_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLY91609.1"
                     /translation="MRREADGRADPLARPERGHDGTPQPRLAITRRTAGLSLAALALL
                     GLDAPAFADDGQVTKSHGLAILAKPALPPGFPYFPYVNPNATKGGDVTLSAVGTFDNF
                     NPFILRGSATLGLMGSFVILPGGSGSGSSVGHIWESLLTSSADEADAGYGHIAESVEV
                     PADKMWVAFNLRAEAKFSDGKPVTAGDVVWTYNTLLEKGRPSFKIQMADVKDVQAESP
                     SRVVWHFKSNQNRELPLILGGLPILPKHFFDGRDFTQPLTDAPIGSGPYRIASFELGR
                     NIVFERRNDWWAAKLSTGIGTNNFDRVRIEYFRDSTVAMEAFKAGQIDLRSENISKNW
                     ATAYDFPAQQKGLVIKHDFTHHLPTGMQGYVMNTRRPIFSNRLVRQALAQVFDFEWEN
                     KNLFYSSYVRTTSYFANSPLASSGLPDADELKLLEPFRKDLPPELFTEPFKLPVTDGS
                     GNNREQLRYALQLLEKAGWTVKDRKLVDKGGQQMTFTILLDEPSLERPSLPYVQNLQK
                     IGIDARVRTVDPAQYQHLVDDFDLDMIMMIFPEGEIPGNELRDYFSAAAAKAKGSYNV
                     PGISDPAVDALVEKVITAQDRTTLTTAARALDRVLLWGWYLVPNWDSRVFHVAYWDRF
                     GHPDKPIREGFNFDTWWVDPAKVKALEAAKQQ"
     gene            complement(4016..4312)
                     /locus_tag="VKQ27_21690"
     CDS             complement(4016..4312)
                     /locus_tag="VKQ27_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HLY91610.1"
                     /translation="MRGGWVYIMANRPDGTLYIGVTSRLARRVAEHNEGVGSRFTARY
                     GLKMLVYAEYHEEIQAAIQRETSLKRWPRAWKVDLITKGNPGWEDLALVGPLPD"
     gene            4339..5409
                     /gene="yejB"
                     /locus_tag="VKQ27_21695"
     CDS             4339..5409
                     /gene="yejB"
                     /locus_tag="VKQ27_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HLY91611.1"
                     /translation="MTAYLIRRLLLVIPTLFGIIAINFIVVQFAPGGPVEQVIAELKG
                     QGGMSSRLGGSGTEIGSGAATYRGARGLDPHMIEDIRKMFGFDKPPLTRFLDMVSGYL
                     RFDFGNSFFRGQPVLQLILQRLPVSISLGLWSTLLTYLISIPLGIAKAVRDGSRFDLA
                     SSAIVLVGYAIPSFLIATLLVVLFAGGSFFQWFPLRGLTSDGASNWSFAARAIDYAWH
                     MVLPTIAVIAGGFASLTMLTKNCFLEEIHKQYTITARAKGASERRVLYGHVFRNAMLL
                     IVAGFPQAFISILFTSALLVEIIFSLDGLGLLGFQAAVQRDYPVMFGTLYIFTLLGLL
                     MQIVGDTLYTVVDPRIDFEARG"
     gene            5406..6434
                     /locus_tag="VKQ27_21700"
     CDS             5406..6434
                     /locus_tag="VKQ27_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLY91612.1"
                     /translation="MKLSPTTHRRLAIFRQHRRGYWSFWIFLVLFFLSLFAEFVANDR
                     PLLIRFDGHWYIPVLHGYSEDTFGSDFLPTEADYTDPEVQQAINSHGWMIWPAIPYSY
                     NTIMKNLTTPAPSPPSWRNLLGTDDQARDVLARVIYGFRLSVLFGFALTIVSSVVGIT
                     AGALQGYYGGALDLLFQRFTEIWSGMPELLLLIILASIITPSFWVLLIFLLLFRWMNL
                     TGVVRAEFLRGRNLDYVRAAKALGVSDIAIMWRHILPNAMVATLTFLPFTLSGSVTIL
                     STLDFLSFGLPPGSPSLGELVAQGRANLQAPWLGITAFVVLGGLLTLLIFVGEAVRDS
                     FDPRRMPK"
     gene            6431..8014
                     /locus_tag="VKQ27_21705"
     CDS             6431..8014
                     /locus_tag="VKQ27_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLY91613.1"
                     /translation="MSLVEVSDLSVAFGKKRVVSGASFALDRGETLALVGESGSGKSV
                     TALSLLQLLPTGGNNPSGSILLDGKQIIGADADTLHQVRGEVAGMVFQEPMTSLNPVH
                     RIGRQVAEAITLHRNMPPKKLRARVIEVLNQAGFADADHRLDAFPHQLSGGQRQRVMI
                     AMALANDPALLIADEPTTALDVTIQAQILKLLARLRIERRLAMLLITHDLEIVRRYAD
                     KVCVMKDGAIVEAGPVAEVFEAPIHPYTCMLLGTVPRGRPVPLRTNCKPLLECDDVKV
                     HFAIRRGVLRRTVGHVRAVDGVSVTVREGETVGLVGESGSGKTTMAMAALRLVGATGQ
                     VTFDGTDIAPFNRRRMCHLRSSMQIVFQDPYGSLSPRMPVGDIVAEGLRVHEPRLSRN
                     QRAQRVAAELEEVGLPADAAERYPHEFSGGQRQRIAIARAMVLKPRFVVLDEPTSALD
                     MSVQAQIVDLLRRLQETYGLAYLFISHDLKVVRALAHRVIVLRQGRIVEEGDADAVFA
                     HPREDYTRKLMAAAFTLDA"
     gene            complement(7969..>8798)
                     /locus_tag="VKQ27_21710"
     CDS             complement(7969..>8798)
                     /locus_tag="VKQ27_21710"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLY91614.1"
                     /translation="FPRQQGSNQAWNHAGNVVAALLVAAATFLVGATAAFWVLGGMAA
                     ASLVTLLIIPADAVDAQRSSGHAMSEPAAAIAQILRDRRMLVLSLALLMFHLGNAAML
                     PLLGQRMAILGQGDATRWLAVCVVVAQLMMVPVAVVAGRMADRIDRAWLMVAACSVLP
                     ARGVIAAVAQDPMWLIPIQVLDALGAGTLGVAMPILVADFTWGSGRTQAALGVASTMQ
                     GVGAAVSTTLAGVLAAKAGWTAAFLGLAVPALFALGLSVRLLGQASSVKAAAISLRV"
BASE COUNT         1489 a         3397 c         2437 g         1475 t
ORIGIN      
        1 gcttcatcta ttcgccggcg atggagaaat tcaagggcaa gccgtggagc ttcgacgccc
       61 tcaacgagtg gctctacaag ccgagcgcct acgtccccgg tacccgcatg tcgttcgccg
      121 gcatcaccaa cgacaagcag cgcgccgacg tgatcgccta cctgcgcacc ctctctgaca
      181 gcccggtgcc cctgcccgca gccaagccgg ctgccgcacc cgctgcccca gcacccgctg
      241 ccgccggcgc ccccgcgacc gccgcccctg cacccgccgc acccgctggc aataccaatg
      301 cggtgcagcc gaacgccccg gcagcctcgc cgacgcctgc cggtgcacct ggcactggtc
      361 cggcggccgg tggctctgcc tcggctcccg ccgcgcccaa cccgccagcc aagccgtagc
      421 gcttggagga cctcgaccgg ttcattgccg gcgccgaggc cgcggctgac gtggccggcg
      481 ccgtcgtccg cccgttcttc cgcgcccccg tcgaggtggt ctccaagtcc gaccgctcgc
      541 cggtgacaat cgccgaccgc tccgccgaac aggccatgcg ggccgtcctg gcggaacgct
      601 tccccgacca cggcatcctc ggcgaggaat acggcgagga ccgcccgggt gcccgctacc
      661 gctgggtcct cgacccgatc gacggcacac gtgccttcgt cagtggccgc ccgaccttcg
      721 gcacgctcat cgccctgatg gacggccccg cccccatcat cggcatcatc gaccagccga
      781 tctccgccga acgctgggtc ggcgcccgcg gccgccgcac catcttccgc ggatcctacc
      841 ccggccaagt cggctgccgt ccatgcccct cgctggcgga agccgaactc tcctgcacca
      901 gccccgagat gcttggcccc gactcggccc gctggcagcg ccttgccgcc gcggtccgcc
      961 ggaactactg gggcggcgac tgttacgccc acggactgct ggctatcggc cagatcgaca
     1021 tcatcaccga gagcaccatg aagctgtggg actgggcagc gctcgtcccg gtggtggaag
     1081 gtgccggcgg caaggtcacc gactggtccg gccagacgct gcgcccggac ggcgatggtc
     1141 gcgttctatc cgttggcgac ccgagactgt taccgcaggc cgtcgcgctg cttaaatagg
     1201 tttcagttgc ctccccccac gcgcggcggc acaccaatcg aaatgtctcg agccacggag
     1261 taagaccttg tccaaaccgt catggccggg cctgacccgg ccatcgctcg ccgcagcgac
     1321 attggtgcga cgagaggctg atggccgagc agatcccctg gccaggcccg aacgcggcca
     1381 cgacggcacg ccgcagcctc gccttgccat cacccgccgc accgccggcc tctctctcgc
     1441 cgccctggcg ctgctcggcc ttgacgcgcc ggcctttgcc gacgatggcc aggtcaccaa
     1501 gagccatggc ctcgccatcc tggccaagcc cgcccttccg cccggcttcc cctacttccc
     1561 ctacgtcaac cccaacgcca ccaagggtgg tgacgtcacc ctctccgccg tcggcacgtt
     1621 cgacaacttc aacccgttca tcctgcgcgg cagcgcgacg ctggggctga tgggctcctt
     1681 cgtgatcctg cccggcggct ccggttccgg atccagcgtc ggccacatct gggaaagcct
     1741 gctcacctcc tccgccgacg aagccgacgc cggctacggc cacatcgccg agtccgtcga
     1801 agtccccgcc gacaagatgt gggtcgcctt caacctccgc gccgaggcga aattctccga
     1861 cggcaagccc gtcaccgccg gggacgtcgt ctggacctac aacactttgc tggaaaaggg
     1921 ccgccccagc ttcaagatcc agatggccga cgtgaaggac gtgcaggccg aaagcccaag
     1981 ccgcgtcgtc tggcacttca agtcgaacca gaatcgcgag ctcccgctga tccttggcgg
     2041 cctccctatc ctccccaagc acttcttcga cggccgcgac ttcacccagc ccctgaccga
     2101 tgcacccatc ggctccggcc cctatcgcat cgcgagcttc gaactcggcc gcaacatcgt
     2161 cttcgagcgc cgcaatgact ggtgggccgc caagctctcc accggcatcg gcaccaacaa
     2221 cttcgatcgc gtccgcatcg aatatttccg cgacagcact gtcgccatgg aggccttcaa
     2281 ggccggccag atcgacctgc gcagcgagaa tatctcaaag aactgggcga ccgcttacga
     2341 cttccccgcg cagcagaaag gcctggtgat caagcacgac ttcacccacc acctgccgac
     2401 gggcatgcag ggctacgtga tgaacacacg ccgcccgata ttctccaacc gcctcgtgcg
     2461 ccaggcgctg gcccaggtgt tcgacttcga atgggagaac aagaacctgt tctacagctc
     2521 gtatgtccgc acgacgagct acttcgccaa cagcccactc gcatcctccg gcctgcccga
     2581 tgccgacgaa ctcaagctgc tcgaaccctt ccgcaaggac ctgccgccgg aactcttcac
     2641 cgagcccttc aagctgccgg ttaccgacgg ctccggcaac aaccgcgaac agttgagata
     2701 tgcgctgcaa ttattggaga aagccggctg gacagtgaag gaccgcaagc tggtcgacaa
     2761 gggcggccag cagatgacct tcaccatcct gctcgacgaa ccctcgctgg agcgcccctc
     2821 cctgccctac gtgcagaacc tccagaagat cggcatcgac gcccgcgtgc gcaccgtcga
     2881 tcccgcgcaa tatcaacacc tggtcgacga cttcgatctc gacatgatca tgatgatctt
     2941 cccggagggc gagatccccg gcaacgagct acgcgactac ttcagcgccg ccgccgccaa
     3001 ggcgaagggc agctacaacg tccccggcat cagcgacccc gctgtggatg cgctggtcga
     3061 gaaggtgatc actgcccagg accgcaccac tttgaccacc gccgcccgcg ccctggaccg
     3121 cgtgcttttg tggggctggt atctcgtccc caactgggac agccgcgtct tccacgtcgc
     3181 ctactgggac cgcttcggcc atcccgacaa gccgatccgc gagggcttca acttcgacac
     3241 ctggtgggtt gacccggcca aggtcaaagc cctggaagcg gccaagcagc agtgaccgcc
     3301 tgcccgcccg ccccgtcatg gccggcccta aaccggccac tgcgcctcaa aggtccactc
     3361 gtgcgaaggg tcgccgaacc ctcccccccg tcatggcctt gagccggcca tcactcccag
     3421 caggcatatc cttgcagcgg gaagccgatg gcccggtcaa acccagccat gacgacacgg
     3481 gacggccgag tgtcccgtcc ccgtgcgaca agccactccg ccgccagccg tcatggtcgt
     3541 ccctgtccct gcgtcgccgc cagcccgtcc agcccccgtc atggccggcc ttgagccggc
     3601 catcactccc cgcaggtgta tcgacgcgtc aggggggagc cgatagcccg gtcaaacccg
     3661 gccatgacga cacgggacgg ccgagtgtcc cgtccccgtg cgacaagccg ctccgccgcc
     3721 agcccatcca tcccccgtca tggccggcct tgagccggcc atcactcccc gcaggtatat
     3781 caatgcgtcg gggggaagcc gatagcccgg tcaaacccag ccatgacgac acgggacggc
     3841 cgagtgtccc gtccccgtgc gacaagccac tccgccgcca gcccatccat cccccgtcat
     3901 ggccggcctt gagccggcca tcactccccg caggtatatc gatgcgtcgg ggggaggctg
     3961 atggcccggt caaacccagc catgcggacg gggggaccca ggcgccccat ccccatcagt
     4021 ccggcagcgg ccccaccaag gccagatcct cccaacccgg gttccccttc gtgatcaggt
     4081 ccaccttcca cgcccgaggc caccgcttca gactggtctc ccgttgaatg gccgcctgga
     4141 tctcctcatg atactccgca tacaccagca tcttcagtcc gtaccgcgcc gtgaaccggc
     4201 tgcccacccc ctcattgtgc tcggccactc gccgcgccag ccggctggtc acgccgatat
     4261 acagcgtccc atcaggccgg ttcgccatga tgtaaaccca gccgccacgc acgccccccg
     4321 tctaccaagg gtcccttgat gaccgcctac ctcatacgac gcctgctgct ggtcatcccc
     4381 accctcttcg gcatcatcgc catcaacttc atcgtcgtgc agttcgcccc cggcggtccc
     4441 gtcgaacagg tcatcgccga actcaaagga cagggcggca tgtcctcgcg cctcggcggc
     4501 agcggcaccg agatcggcag cggcgccgct acctaccgag gcgcccgcgg cctcgatccc
     4561 cacatgatcg aagacatccg caagatgttc ggcttcgaca agccgccgct cacccgcttc
     4621 ctcgacatgg tcagcggcta cctccgcttc gacttcggca acagcttctt ccgcggtcag
     4681 ccagtgctgc agctcatcct ccagagactc cccgtctcca tttcgctcgg cctctggtcc
     4741 accttgctca cctatctcat ctccatcccg ctcggcatcg ccaaggccgt tcgcgacggc
     4801 agccgcttcg acctcgccag cagcgccatc gtgctcgtcg gctacgccat cccgagcttc
     4861 ctgatcgcca cgctcctcgt cgtgctgttc gccggcggca gcttcttcca atggttcccg
     4921 ctgcgcggcc tcaccagcga tggcgcctcg aattggagct ttgctgcccg tgccatcgac
     4981 tacgcctggc acatggtgct tcccaccatc gccgtcatcg ccggcggctt cgccagcctg
     5041 accatgctga ccaagaactg cttcctcgag gaaatccaca agcaatacac catcaccgcc
     5101 cgcgcaaagg gcgccagcga gcgccgcgtg ctttacggcc acgtcttccg caacgccatg
     5161 ctgctcatcg tggccgggtt cccccaggcc ttcatctcca tcctgttcac ctccgccctg
     5221 ctggtggaga tcatcttctc cctcgacggc ctcgggctgc tcggcttcca ggccgctgtg
     5281 cagcgcgact atccggtgat gttcggcacc ctctacatct tcaccctgct cggtctgctg
     5341 atgcagatcg tcggcgacac gctctacacc gtggttgatc cccgcatcga cttcgaggcg
     5401 cgcggttgaa gctatcgccc acgacgcacc gccggctcgc gatcttccgc cagcatcgcc
     5461 gcggctactg gtccttctgg atcttcctcg tgctgttctt cctcagcctg ttcgccgagt
     5521 tcgtcgcgaa cgatcgcccc ctgctgatcc gcttcgacgg ccactggtac atccccgtcc
     5581 tgcacggcta cagcgaggac accttcggtt ccgacttcct ccccaccgaa gccgactaca
     5641 ccgaccccga ggtgcagcag gccatcaaca gccacggctg gatgatctgg cccgccatcc
     5701 cgtactccta caacaccatc atgaagaacc tgacgacacc agcgccctcg ccgccaagct
     5761 ggcgcaacct gctcggcact gacgaccagg cacgcgacgt tctggcccgc gtgatctacg
     5821 gcttccgcct gtccgtgctg ttcggcttcg ccctgaccat cgtcagctcc gtcgtcggca
     5881 tcaccgccgg cgcactgcaa ggctattacg gaggcgcgct cgacctgctg ttccagcgct
     5941 tcaccgagat ctggagcggc atgcccgaac tgctgctgct catcatcctc gccagcatca
     6001 tcacccccag cttctgggtc ctgctcattt tcctgctgct gttccgctgg atgaatctca
     6061 ccggcgtcgt ccgcgcagaa ttcctgcgtg gccgcaatct cgactacgtg cgcgctgcca
     6121 aggcccttgg tgtttccgat atcgccatta tgtggcgtca catattgccc aacgcgatgg
     6181 tcgccacgct gacattcctg cctttcaccc tctccggttc ggtgacgata ttgtccacgc
     6241 tcgacttcct cagcttcggc cttccccccg gctcaccctc gctcggcgaa ctcgtggcac
     6301 aaggccgcgc caatcttcag gccccctggc tcggcatcac cgcattcgtc gtgctcggcg
     6361 gcctgcttac cctcctgatc ttcgtcggtg aggcggtgcg cgattccttc gatccgcgca
     6421 ggatgccgaa atgagcctcg tcgaggtcag cgacctttcc gtcgcgttcg gcaagaagcg
     6481 cgtggtgagc ggcgcatcgt tcgcgctcga ccgtggcgag accctggcgc tggtaggcga
     6541 gtcaggctcc ggcaaatcgg tcaccgccct gtccctgctc cagcttctgc cgacgggtgg
     6601 taacaatccc agcggcagca tcctgctcga cggcaagcag atcatcggtg ccgacgccga
     6661 tacgctgcac caggtccgcg gcgaggtcgc cggcatggtg ttccaggagc cgatgaccag
     6721 cctgaacccc gtgcaccgca tcggccgcca ggtcgccgag gcgatcacgc ttcaccggaa
     6781 catgccgccc aaaaagctgc gcgcccgcgt catcgaagtg ctgaaccagg ccggtttcgc
     6841 cgatgccgac caccggctgg acgccttccc ccaccagcta tcgggcggcc aacgccagcg
     6901 cgtgatgatc gccatggcgc tcgccaatga tcccgccctg ctgatcgccg acgaaccaac
     6961 caccgccctc gatgtcacca tccaggcgca gatcctcaag ctgctcgcgc gcctgcgcat
     7021 cgagcgccga ctcgcgatgc tgctcatcac ccacgacctg gaaatcgtgc gccgctatgc
     7081 cgacaaggtc tgcgtgatga aagacggcgc catcgtcgag gccggtcctg tcgcagaagt
     7141 gttcgaggcg cctatacacc cgtatacctg catgctgctc ggcaccgttc cccgtggccg
     7201 ccccgtcccc ctgcgcacca actgcaagcc gctgctcgag tgcgacgacg tcaaggtcca
     7261 cttcgccatc cgccgaggtg tcctgcgccg cactgtcggg catgtccgcg ccgtggatgg
     7321 cgtctcggtc accgtgcgcg aaggcgagac cgtcgggctg gttggcgaat ccggctccgg
     7381 caaaaccacg atggcgatgg ccgcattgcg cctggtcggc gccaccggcc aggtcacgtt
     7441 cgatggcacc gatatcgcgc ccttcaaccg ccgcaggatg tgccatctgc gctcgtccat
     7501 gcagatcgtg ttccaggacc cttacggcag cctgtcgccg cgcatgccgg ttggggacat
     7561 cgtcgccgaa ggcctgcgcg tccacgaacc gcgtctcagc cgcaaccagc gcgcgcagcg
     7621 cgtcgccgcc gaactcgagg aagtcggcct ccctgccgat gccgccgaac gctatccgca
     7681 cgaattcagc ggcggccagc gccagcgcat cgcgatcgcc cgcgccatgg tgctgaagcc
     7741 gcgcttcgtg gtgctcgacg aacccaccag cgcgctcgat atgtcggtgc aggcgcagat
     7801 cgtcgatctg ctgcgccggc tgcaggaaac ctacggcctc gcctatctgt tcatcagcca
     7861 cgatttgaag gtggtgcgcg cgctcgccca tcgtgtgatc gtgctgcgcc agggtcgcat
     7921 tgtggaagaa ggcgacgccg acgccgtgtt cgcccaccct cgcgaggact acactcgcaa
     7981 gctgatggcg gccgccttca cgctggacgc ctgacccagc aatcgtaccg ataatccaag
     8041 cgcgaacagc gccggcaccg ccaggccaag gaacgctgcc gtccaccccg ctttcgccgc
     8101 cagcacgccg gccagtgtgg tggacaccgc cgcgccaact ccctgcatcg tcgatgccac
     8161 acccaatgcc gcctgcgtcc gtccgctgcc ccatgtgaaa tccgccacca ggatcggcat
     8221 cgccacgccc aatgttccgg ctcccaatgc atccaacacc tgtatcggaa tcagccacat
     8281 cggatcctgc gccaccgctg cgatcacgcc gcgcgccggc agcacggagc acgcggcaac
     8341 catcagccat gcccggtcga tccgatcagc catgcggcct gcgacgaccg cgaccggcac
     8401 catcataagc tgcgccacca ccacgcaaac cgccaaccag cgggttgcat cgccctgccc
     8461 gagtatcgcc atccgctgcc cgagcagcgg caacatcgcc gcattgccga gatggaacat
     8521 cagcagagcc aggctcaata ccagcatgcg tctatcacgc agtatctgcg cgattgcagc
     8581 cgccggctcc gacatggcat ggccactgga tcgctgagcg tcgaccgcat ctgcaggaat
     8641 gatgagcagt gtgacgagac ttgccgccgc cattcccccc agcacccaga atgcggcggt
     8701 cgcaccgacc aggaatgtcg cggccgcaac cagcaatgcg gcaacaacat tccctgcatg
     8761 gttccaggcc tgattgctgc cttgctgtct tgggaacc
//
